LOCUS       DAORNH010000001        54970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_447_length_54970_cov_2.697041, whole genome shotgun sequence.
ACCESSION   DAORNH010000001 DAORNH010000000
VERSION     DAORNH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 54970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54970
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_54970_cov_2.697041"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    421..466
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<467..1563)
                     /locus_tag="PK264_00005"
     CDS             complement(<467..1563)
                     /locus_tag="PK264_00005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRD74311.1"
                     /translation="MGFFADSLNRIQPSATIAISTKARELKAKGRDIITLSQGEPDFE
                     TPQNIKDAAVKAIASGKTRYTDVDGIPELKDAIVAKFKRENNLTYTRSQITVGTGGKQ
                     VLYNALMCTINPGDEVVIPAPCWVSYADIVMLGDGKPVFAMTRLEDGYKLKPDTLEKA
                     ITPKTKWFLFNAPSNPSGAAYSRAELKALTDVLLRHPHVWVLTDDMYEHLLYDGLKFV
                     TPAEVEPKLYERTLTMNGLSKAYCMTGWRLGYAGGPEPLIKAMCKLQSQSTSNPSSIT
                     QWAGVEALNGPQDFIPRNNAIFKERRDLVVSMLNQAKGLKCPKPEGAFYVYPSCAGTI
                     GKTSKSGKVIKNDEDFVTALLEEEGVAAVHGA"
     gene            1746..2816
                     /locus_tag="PK264_00010"
     CDS             1746..2816
                     /locus_tag="PK264_00010"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRD74312.1"
                     /translation="MAASRPLLALAMGDPAGISPELTAKLLADEAVAAAADLVVIGDR
                     RVLAAGESVAGVKARTEPWIPRKAVGGGHTFVDLAHLDPTRIARGASSKEGGAFALRN
                     FRTALALGRDGVVDAVTFTPFNKHAMKLAAGSYEDEIAVISEVTGTKGHGREFNILGA
                     LWNARVTSHVPLKDVAKYITREAVVNGLVLTDRCMKEAGVARPRIAVAGLNPHAGDEG
                     NFGREEIDVIAPAVVEAKAKGIACEGPFPSDTVFLRAKRGDFDAVQTMYHDQGQIAMK
                     LMGFESGITLIGGYAFPVCTPAHGTAYDIAGKGIADLGASRNAILIAAKMVSQRKGAP
                     ASSSDRAAALARSLDAAKGQAA"
     gene            2834..>3250
                     /locus_tag="PK264_00015"
     CDS             2834..>3250
                     /locus_tag="PK264_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD74313.1"
                     /translation="MSTAAQPDSPGIALVTGAAHRIGRAIALHLGRLGWSVAVHFNGS
                     REAAEAVAAEVSKCGGRSIALKADLASADEVRDLLPRCVAAIGAPTCLVNNASMFEDD
                     RIESLDARLWDALLAVNLTAPVLLAQAFQRHLPAGQH"
     assembly_gap    3251..3260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3261..3591
                     /locus_tag="PK264_00020"
     CDS             <3261..3591
                     /locus_tag="PK264_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD74314.1"
                     /translation="AIVNIIDQRVWRPSPEFFSYGVTKSALYAVTRMLAQAMAPRIRV
                     NGIGPGPVLKSIHQSEAEFSAEWKSTLLARPTSPDEVAAAVAFILSQPSMTGQMIALD
                     AGQHLSY"
     gene            3596..4453
                     /locus_tag="PK264_00025"
     CDS             3596..4453
                     /locus_tag="PK264_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD74315.1"
                     /translation="MLPLAAFTTHPSLFSLFGLVVGDGGARVEQGITYGPDARHLLDI
                     WRPRRAPDAGPVVVYFYGGGWTSGCRAGYGFVGGAIASIGATCVIPDYRLYPKVRWPV
                     FQEDAAAAVAWVARNVPGIERRPLVLMGHSAGAHLAAMLALDPRWLRAAGANRPVDGL
                     IGLSGPYWFEPTTWPTTAHIFADATSKDEPRPVFHAGPHAPPALLVHGEADDLVKPHN
                     THELAAALEAAGRPVTKLVVPGAGHRKTLLGIARPVRWRTDARGPIAEFLRGIDGNGI
                     TSLHATDAR"
     gene            complement(4437..5054)
                     /locus_tag="PK264_00030"
     CDS             complement(4437..5054)
                     /locus_tag="PK264_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74316.1"
                     /translation="MRVPLAILFSILAASAATAQCPTRESAAKGFILKRDDGVVTEVV
                     GGHGGIVQSIERFEKPMRPQIGYSHYRGLIALVSESSSTRRQVLFDQDLSAIFPLKSG
                     TTHNLSHSMASKSLVNGTDEPTKTAKMRLTIGDTTSEVEIGGCRYVTIPINKFIRYSD
                     GMTLRLEEQWSADIEFVLARHIVTTLAGKEPTMFALTYVSISVRP"
     gene            complement(5088..5690)
                     /locus_tag="PK264_00035"
     CDS             complement(5088..5690)
                     /locus_tag="PK264_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74317.1"
                     /translation="MLRWLAALMIVSLPVGGAEAACPTWTTSPKGFVLTRMDGMTLEV
                     TGRGNDIVSMSERYPDPSHLPIEKSYIRGLLPNTSKSKAKLATVNYDQSPLAIFPLLP
                     GAAHDLGYKISSDGQPSITARLALNIATERSTVAIGPCSYPTIAVTKAVTFSNGHEYV
                     NQEFWSEELELVLRTVVNSRLAGKEPVRLTLEFTAISARP"
     gene            complement(5702..7711)
                     /locus_tag="PK264_00040"
     CDS             complement(5702..7711)
                     /locus_tag="PK264_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRD74318.1"
                     /translation="MSRAEMDTLGWDACDIIIVTGDAYIDHPSFGMAIIGRLLEAHGF
                     RVGMIAQPDWRSPADFMRLGPPRLFFGVTGGNMDSMVNRYTSDRRIRSNDAYTPGGES
                     GKRPDRAVIVYAQRCREAFRDVPVVIGGIEASLRRIAHFDYWSEKVRRSVLADAKADL
                     LIYGNAERAIVDVAHRAAAGVELSAIDGIRGIAVMKSSLPDGFTEIAADDVESADEGA
                     ARANGPAVVRLPAYEQVQDDKDAYARASRVLHRESNPGNARGLVQRHGDRDLWITPPP
                     IPLSTEEMDGVYGLPYARGPHPSYGDAKIPAWEMIRFSVTIMRGCFGGCTFCSITEHE
                     GRIIQSRSEDSVLREIEEIRDKTKGFTGIISDIGGPTANMYRMACKDKRVEALCRRPS
                     CVYPGICPNLDTSHDALIGLYRKARELPGIKKVMVASGVRYDLAVESPEYVNELVMHH
                     VGGYLKIAPEHTEDGPLAKMMKPGIGTYDRFKELFDRAAKDAGKEYYLIPYFIAAHPG
                     TRDEDMLSLAVWLKKNGFRADQVQTFLPSPMATATAMYHTGLNPLRGVRRGGSEKVEA
                     VKGLKQRRLHKAFLRYHDPENWPLLREALKRMGRADLIGPGKQHLIPAGQPAGFVPRK
                     SGEPSRQRPGAAPVARRGTFSTTGVFPPIHGAPDRTKAKPRRPKR"
     assembly_gap    8055..8266
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8267..8654
                     /locus_tag="PK264_00045"
     CDS             <8267..8654
                     /locus_tag="PK264_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRD74319.1"
                     /translation="RQGPSFDQKVLWVYRRAGLPVEVLREHEGWLQIRDSEAGTGWVL
                     SSLVSARRTALVAPWEVKPGVAPPQIEIRSEPSAASPLVALVEAGVIASLVSCDRRWC
                     RIAINDIRGFVDQKRLWGVYEDEVLK"
     gene            complement(8664..9029)
                     /locus_tag="PK264_00050"
     CDS             complement(8664..9029)
                     /locus_tag="PK264_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74320.1"
                     /translation="MLPPLYELTLFLALSLAVTLYALIASGHFPAEGRRETLASPLGL
                     VIIWGSMAIAVAALALALLLAWRRMPLAIAVIAFCAMGLVAPLVMKPMPDSFVDGRRG
                     LLLTAGAAAALAALAWARI"
     gene            complement(9058..10416)
                     /locus_tag="PK264_00055"
     CDS             complement(9058..10416)
                     /locus_tag="PK264_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HRD74321.1"
                     /translation="MTIQESRLRLQTLIKLRWFGVAGQAATVLLVYFGLGYSLPIALC
                     FVLIALSAWVNIYLRIKFPMRFRLGASLASALLACDIVGLAALLYLTGGVQNPFVFML
                     VAPVTVSAATLPPRNTIVLGLLAVVMAALLVEWHLPLPWGAGEAFVLPRVYKGGIFAS
                     VVSAMIFLGIYAARLTKEARQMTAALAATEHVLAREQRLHALDGLAAAAAHELGTPLS
                     TIVLVTKELERETAGQPAIEEDIKLLRAQADRCREILRTLTRKPNEQDPHHASLPLTQ
                     LLQEAAEPYRKGVKRITIATAAAVDGPGAMREPIAERRPGLIYGLGNIIENAVDFASG
                     EVTIDARWSDRNVEVRIADDGPGFAPGVIEALGEPYVTTRPQGQQQKQGSEAAGLGLG
                     FFIAKSLLERSGAHLAFANRAAPATGAVVHVTWPRIAFDISQSKGAARPAGEIIAATA
                     AA"
     gene            complement(10463..10981)
                     /locus_tag="PK264_00060"
     CDS             complement(10463..10981)
                     /locus_tag="PK264_00060"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRD74322.1"
                     /translation="MSFETLKQWLVALVREHDWLAGPVCFFLGFGEGIPVLSLLLPSS
                     ALFLGIGAVHGAAGGVFWTTWLWASIGAILGDCVTYAIGRVFKDQSVSIWPCSKRPEW
                     LIQSRVMIRDWGALSVLGGKFLGLLRPFIPVVAGMFVMPLWKFVPASIVSSFVWAAAF
                     LGPGYGLARLAL"
     gene            complement(10988..12640)
                     /locus_tag="PK264_00065"
     CDS             complement(10988..12640)
                     /locus_tag="PK264_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD74323.1"
                     /translation="MAKAVREGKAALDPVTVEVIGNALASIAEEMGETLVRASYSTNI
                     KERRDSSTCLFDAAGRTLCQAMHIPMHLGSLIGIVEAIYRRVPMAEIAEGDVFIGNDA
                     YTGGGTHLPDLVLAEPIFFEGQLVGWATNTAHHADFVDRGHAHIFQEGIRIPPVRLYR
                     RGELVTDVQEMILLNMQVPDERLNDLRAQMAANRLGVQRFQALCRRYGRDTLMSAAAA
                     LLDYAERLTRAGIRTIPSGTYRFEDVFDSEEVDGNLTFKVEIVVKDDEIALTFDAPRQ
                     VRAGFNLVETGLLATVYYALKTVIGPDIPPNAGFFRPITVTAPKGSVLNAAPPAAVNS
                     RLDAGQRVVDLIHGALATAIPERVTAAHNGACVYADFSGVDPRNGRYYVYLETIGGGF
                     GARATKDGLDGVHVHLTNTSNLPVEALETEYPLTIERYELVQDSGGPGRMRGGMALRR
                     VVRAEHDECRTYIIGGRATVAPWGLDGGHRGGTAHFNFNAPSEAPDRGRACLAKGSSV
                     EIVTPGAGGYGEPRLRDPAMLERDVADGKVSTSAARSAYGRA"
     gene            13780..15801
                     /locus_tag="PK264_00070"
     CDS             13780..15801
                     /locus_tag="PK264_00070"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HRD74324.1"
                     /translation="MTLPLKLLSPLDLGHTSLANRLIMGSMHTRLETLDRPLDRIARF
                     YAERARGGAGLIVTGGFSPNDDGLIEPNGPVLSSSHQLGEHRPVTDAVHAAGGKIALQ
                     ILHAGRYATHELAVGPSDVASPINPRKPRRMTEADILRTIEDFATCAALAREAGYDGV
                     ELMGSEGYLINEFTAPRTNDRVDAWGGSLDNRLRFPVAVMRRVRERVGADFIVMFRIS
                     AIDLVDGGLTSEEIVAQARLVASAGASIINTGIGWHEARIPTIAQKVPRSAWGFAARR
                     IKDAVAIPVVVSNRINTPDEAERILTRGDGDLVSMARPWLADPKFGLKAREGRANEIN
                     VCIACNQACLDYIFSGRVATCLVNPRAARELEFDAPPPSNRKRVAVIGAGPAGLAFAT
                     TAAERGHAVTLYEAEARIGGQLNLARTIPGKQEFDETVRYWRTLIEKHGVEVRLGARP
                     TAGALSAGRFDEVVVATGVTPRRLDIPGADHPSVASYANILSGKRVAGRRVAIIGAGG
                     IGFDVAELLSSPHASGALSAESFATEWGIDRSVAGRGGLATPAEETPPREIVMLQRKT
                     GRMGRSLGVSTGWILRLQLAKRKVAQVTGAAYRRIDDQGLHVTVGGAERVIPADTIVV
                     CAGQESQRSLADELVVLGIAPHVIGGADVAAELDAMRAIDQGMRLAYAL"
     gene            complement(15814..16383)
                     /locus_tag="PK264_00075"
     CDS             complement(15814..16383)
                     /locus_tag="PK264_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HRD74325.1"
                     /translation="MSHGAHGHGEGGNKTIAIFISILALCLAIAETMAKSAQTDALGY
                     NIKAADTWAFFQAKTIRRTTIEAMAETMEIDAKLARDPATKQLLDKRIGDWRKTAARY
                     RSEPETGEGSVELMAKAKDAERKRDLALAKYHHYEVSSAAFQIAIVLASAFAITTAIY
                     LFWVAAGFSGVGVIFFLIGLLVPKSVHLF"
     gene            complement(16488..17588)
                     /locus_tag="PK264_00080"
     CDS             complement(16488..17588)
                     /locus_tag="PK264_00080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD74326.1"
                     /translation="MASTSGTRFGIFSLSQFPDQAKRVESFDNDLMFFESAEALGYDE
                     IWIAEHLFSTYGVVTSTQVYAAAIAQRTKRIRIGMAVVVIPFNHPLRTASDFALVDVL
                     SHGRLDFGIGRAYQPHEFVGLGVPMEESREMLSEGIDIVLRAWKDEKVSHKGRYWTIP
                     EPVEVLPKPVQQPHPPVFQATVSPESFEQAARSGFNLQMASPFTYRTYRETWRDELAR
                     SLARHDEVCRGMGRDPKTVRKMILLPFFVHEDQAKARAIYKPHVEWFYAKVTANQLSG
                     APKAGVVKGYELTMSEGKKTREMGYLAFDKLIENGAAVVGDPKECVAQLRDLKSRLGI
                     TDFALWFNIGGIDPAHVTRAMRLCAEKVMPEV"
     gene            17748..18857
                     /locus_tag="PK264_00085"
     CDS             17748..18857
                     /locus_tag="PK264_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HRD74327.1"
                     /translation="MPCPAFLSHPIFLASGFGRHHPLSIPRQSLVIGLAEALGWLDRA
                     DIEPCPLPDIGVLARLHEPAYLEALETAARAGVASREARERYNLGTMECPIFPGLWDR
                     ARATVGGSIRAAELAMSGRTAFHPAGGTHHGMTARASGFCYFNDPAFAVLSFLDLGAA
                     RVAYIDIDAHHGDGVEAMFAPDPRVMTLSIHEEGRWPGTGRLGDRARGRAHNMPVPRD
                     ISDSEFLYLIDEAVLPLAETFAPGAVVVTCGVDTLAGDPLSGMALSNTAVWDAVDRIA
                     CLGRPTVILGGGGYNPWTLARAWTGVWGRLCGEGTAIALPEAARALLAPLSVDLVEPE
                     DIDPRWLSRLEDTPHPGPIRDPIRHIAEKMRAPSQ"
     assembly_gap    19134..19148
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19149..20883
                     /locus_tag="PK264_00090"
     CDS             <19149..20883
                     /locus_tag="PK264_00090"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRD74328.1"
                     /translation="GTIVLAKEIHQPLSLIVDGQQRLITLTILLALLRDWMPTLGSVT
                     LDVDTAITSVKDEPRLSPRPADADFFREYVQDMGATRRHPPNWVMTEVGLDDESEAPA
                     VASGDDEDPYVSDPAAGEPMADALDDIDPPQPIDSDEVVIDVPHVGEEPVIQGMPAAH
                     DSQRLFIENRDRLRARLEALAKQKRVNLADLASFILDRCIVVVVAVQRSDEAHRIFSV
                     LNTRGLDLSDADILKSEVLGAITDPGAQRTCGAIWEYAEQELGRDEFQSLLTHIRMIG
                     LKRRPRRAIVDELRDHYKPAEAPVDFVENTVQPMAAILAGLNAAEDAGKEISIQGADS
                     ATMERIRRYLIYLGWIPNVSWKPPAIAFFMRYGDNPSAILRFLKMLDRLSFGMLILKS
                     DEGERISRYSRVIEAIEAGQNLFSPKSSAMKLEQRQRQIILNRISNPYFGIQSYCGLV
                     LRRLDAELMVGPLPSYRDSTVEHVLPQNPRLTSQWLDWYPGPPEDPAKGVVENCQRLG
                     NLILLSKTDNNCAANNQLSIKLDCYFGENNTSPFSLKDEVASLRSFDQKVLDRLHERR
                     VRRIRHLWGLL"
     gene            complement(20922..21686)
                     /locus_tag="PK264_00095"
     CDS             complement(20922..21686)
                     /locus_tag="PK264_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD74329.1"
                     /translation="MKSPFDLTGQVALVTGSSRGIGRSIAETMANLGAKVIVSSRKAD
                     ACEEVAGAIRKAGGEAHVIPCNIGRKGEVEALIKATLDKLGRLDALVCNAAVNPYFGP
                     LAGIADDQFDKIMATNIKSNLWLANLAAPGMAERGGGSITIVSSIGGIRGSSMLGAYG
                     ISKAADFSLARSLAVEWGPKNIRCNCIAPGLVKTDFARALWEDEARLKTRLATTPLRR
                     IGEPDDIGGIAAFLASKAASFITGQVIVADGGVTIA"
     gene            complement(21776..22432)
                     /locus_tag="PK264_00100"
     CDS             complement(21776..22432)
                     /locus_tag="PK264_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD74330.1"
                     /translation="MLDRGLRALLAGAVLALSVSAALGQSLSDFKRSWETCQGADLKA
                     RVAACTELLEDRLSDGERVIVHGNRAIARRRLGDYPGAFADYGEALRIDPKNPYIRNN
                     RAWAYYKSGRFEEGLADIELALAVAAPSAPWLDTRGHIYQALGRSARAYDDYHTAMRI
                     GGEIWVKRYQCGMRNAGHFKGDLDGKLTAALLTALRACVADRKCDPLPDDDECRQLTS
                     "
     gene            complement(22440..23090)
                     /locus_tag="PK264_00105"
     CDS             complement(22440..23090)
                     /locus_tag="PK264_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD74331.1"
                     /translation="MRPFRAAAIAVALVLGTAGPASAGPYEECLLNPNLTADTVRAVD
                     ACTEVITNPAWPQEVIAQAFANRALAYSVMGDYERAIRDYNSAIDIAPNYATALNNRA
                     WAYFRSGRPELGLADVEKSLRLNPLSPHAYDTRAHIRQSAGDTARALRDYEMSLNLGG
                     ERMIKLYQCGLAEAGLYKGQQDGKDSKELREALSKCVLDKKCDPLPQDEQCRPRAM"
     gene            complement(23195..23536)
                     /locus_tag="PK264_00110"
     CDS             complement(23195..23536)
                     /locus_tag="PK264_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74332.1"
                     /translation="MSRKTRKIPLHEIAHARAGDKGNTSNVSVWAYDPGDYPLIKAQL
                     TADRLKAAFPGLIKGRVDRYELDHLHGLNFVLADALEGGVNTSLGLDSHGKSWSFLVL
                     GLEIEVPDNRA"
     gene            complement(23533..24879)
                     /locus_tag="PK264_00115"
     CDS             complement(23533..24879)
                     /locus_tag="PK264_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HRD74333.1"
                     /translation="MLRLGCGSGFSSDRLGPAIDLAEKGRLDYLVFETIGERTMAFGH
                     RDRKADPRRGYNPQLGQRMRGVLKACRRNGTRVVTNMGVANVAAAAELTVAIARELGL
                     SGLKVAAVEGDDVTAMIGPETRLFDHHDKRVDEVGLAVVGANVYLGVDAILPALEAGA
                     DVVITGRVSDPSLFLAPIMHRCGWRRDDWERLGRGTLVGHLMECGMQITGGYYADPGR
                     KDVPGLARCGYPIAEVEEGGEAVITKLADAGGMVSPRTVKEQLLYEVHDPRAYLTPDV
                     TADFSKVNVGEAGANRVAVSGASGRAQPETLKVTVGFDGGFYAEAGVSYAGPNAVARA
                     RLAANILRERLGPDEIGSAPLRIDLVGVNALHATADLAIDDPDTSMPADVRIRAAMRS
                     ADRAACEMLLWETESLLCCGPAGGGGYRGQITPSVVTHSALIPRGAVKPSVRMLVA"
     gene            complement(24937..25920)
                     /locus_tag="PK264_00120"
     CDS             complement(24937..25920)
                     /locus_tag="PK264_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD74334.1"
                     /translation="MMRMKLSSLGLAASLIAGAATVAFAQGFPSRPINLIVAFPAGGS
                     TDIGARIVGAIAEKAFKQPVVIVNKGGAGGQIGWTELSRAKADGYTIGYVNLPATHTV
                     VLDPERKAIYDLDSLVPVINQVLDPGIVWVRADSPFKSFKQLLEEAKAKPGTIRAATT
                     GILSDDHLAILMSEEAMPGASFRMVHLAGGAAQLKEVLAGNIDVAFDNVGSIVKQVKA
                     GQLRGLAVLDTQRSKFLPDVPTSVELGFKTVISSSTRGIAVPKGTPADVVKALADTFK
                     AAMADPDHVSKLEEQGLAIKVMIGDEYTKYYRDNYALSKKYVEWAKSRPAK"
     gene            complement(25978..27474)
                     /locus_tag="PK264_00125"
     CDS             complement(25978..27474)
                     /locus_tag="PK264_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD74335.1"
                     /translation="MEGLTFLLNGMIAASTGQNLLFAVAGCFLGTLIGVLPGVGPAAG
                     TAILIPLTFKLDATAGIIMLCAIYYGAMYGGTITSVLINVPGEAASVVTCLDGYEMAK
                     QGRGGTALGIAAIGSFIGGSIATLALVVVAPYLAQLGLRFGPPEFFGLMLLGLCLVTA
                     LAGRSIVAGLLMAIVGLLLSLVGQDPTRGAPRFTFDIVALYDGVDFIPVVMGLFGVGE
                     LLSTAEKPYRDVLKTNLTRLFPTAAEWKRSIGAIFRGSGIGLFMGLIPGVGAIVPTFM
                     AYILEKKLSRHPEQFGKGAIEGVAAPETANNAYANASMIPLLTLGIPSSPTIAIIMGA
                     FIMKGLAPGPFLFSEKPDLVWAVIASFFVGNVILLVMNLPFVGLWARILHIPWQYICV
                     GTLLFCILGAYSLRQSVFDIWVMLAFGIIGFTMRKLDLPTAPLILGLILGKELEKNLR
                     TSLEMSGGDFSIFFTRWLSLALLIMSAMIIIGSALSLAPKEVRESTAD"
     gene            complement(27478..27939)
                     /locus_tag="PK264_00130"
     CDS             complement(27478..27939)
                     /locus_tag="PK264_00130"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRD74336.1"
                     /translation="MRRAWIIASALLAALFGYTVWESRKYAYLDALGPGPGFFGIWLG
                     GVGVLLALFLIAEVVRGGAFLDGDARVLPALADVPRPLAILVGIALVAASVEYVGFRL
                     ATLVFIAVLLPLLGVRNWIAIPVLAVAGSWGVFHVFYHWLKVPLPLGELGI"
     gene            complement(28008..28433)
                     /locus_tag="PK264_00135"
     CDS             complement(28008..28433)
                     /locus_tag="PK264_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4174 domain-containing protein"
                     /protein_id="HRD74337.1"
                     /translation="MRRSALITALLAIMLTPSAAFAMSGYMWKKRPLVVFAPDGNASA
                     LASQRAIVAGARGGFAERHMVVVWVVGDSVSSELGGGPGLSAADLRRRYGVARGSFRA
                     ILIGKDGGAKISSSKPLAAGMLFSTIDAMPMRRDEMRGR"
     gene            complement(28682..30016)
                     /gene="aroA"
                     /locus_tag="PK264_00140"
     CDS             complement(28682..30016)
                     /gene="aroA"
                     /locus_tag="PK264_00140"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRD74338.1"
                     /translation="MTHATNTPSPLQSLRARALGGTVSLPGDKSISHRALMLGALATG
                     TTRITGLLEAEDVINTAKAVTALGAPAAKSGKSWTVKGRGVGGLAEPAGPLDFGNSGT
                     GSRLMLGVIAGHDMRVRFVGDASLSRRPMGRVLTPLKQMGLEVVEGSDRMPLVVRGTS
                     SLAPIEYESPVPSAQVKSAILLAGIHAAGATTVVEKEATRDHSERMLRYFGGTVETVE
                     RGGKTAITVRGDAELAGRDLIVPGDPSSGAFLAAAALIAPGSEVTLKGVLVNETRVGF
                     YTTIAEMGADIQILERREQCGEPVADIRVRHSKLKGVHVPAARAPSMIDEYPVLSALA
                     AYADGETRMDGLKELKVKETDRLAATAAGLAANGVGARVDGDSLLVTGGRGVKGGGLV
                     ATHMDHRIAMSFLILGLGADRPVAVDDVTFVATSFPEFRPLMETLGAKFATP"
     gene            complement(30086..30865)
                     /locus_tag="PK264_00145"
     CDS             complement(30086..30865)
                     /locus_tag="PK264_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD74339.1"
                     /translation="MRWIRYSLAGVTSYGIVEDELIAEVTGSPFAEHARTGRRVPLAD
                     VKIELPVMPQTFYCVGLNYAEHVTAAAKKMGLPVTLPTQPDVGYRAQNALIAHGEDVI
                     IPAHATEKVQYEGELVVVIGKRAKGLSKADALSCVLGYTIGNDVSERSWQKSDKTLWR
                     AKNSDTFKPMGPWIETDVRLDDLITICRVNGRETERFKTNDMIFGIADFIAAITSIAT
                     LHPGDVIWMGTDGASPNLVSGDVVEIEITGIGMLRNRFIRH"
     gene            complement(30930..31490)
                     /locus_tag="PK264_00150"
     CDS             complement(30930..31490)
                     /locus_tag="PK264_00150"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRD74340.1"
                     /translation="MATAFAGLITMATSQYSAVAQTAKASPPAPSAVAPPSELGLWID
                     HTGRGAVTIAPCGDKLCGHIAWLKDPNDEKGQPLRDLNNPVKGKRGQTICGLQILGDL
                     KKSGRVWDYGWIYDPEKGEKYDLEIRLKSADTLQVTGYLGVKFLSETFLWKRAPEGMF
                     ACPPPAVSPAPVPNAAPAPVPQPPKR"
     gene            31812..32939
                     /locus_tag="PK264_00155"
     CDS             31812..32939
                     /locus_tag="PK264_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD74341.1"
                     /translation="MILKLAFRNLVHDRIRLTVTLVGILFSIVLVAVQLGLYLGARKM
                     IVGMIENAKADIWITSFGAKSFEEAGQLTGRERYGALSTPGVDAVTPIVVSFAEWRKP
                     AGGSTLIVLVGTDPADGGLAPWNVVDGVGSDLTTPDAVIADRTYIRDLGVSRLGDTAQ
                     IESGKVKLAALTSGIRSFTMAPYIFTTLNRSRSLLAMPQDQATFLLVKASDPADVPAL
                     QSRLSARLPGSDVLTKAQFIGRSLDHWLFATGAGVALIGGAILGLIVGTVIVAQTLYS
                     STKDHLNEFATLRALGSSSGYIHKVILWQAGLSALLGYLIGMAVAMVIVHFSEQTALP
                     IVMTPWLAAGLLALTVFMCAISAVSAIMKVTRIDPAMVFAR"
     gene            32936..33616
                     /locus_tag="PK264_00160"
                     /pseudo
     CDS             32936..33616
                     /locus_tag="PK264_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            33822..34907
                     /locus_tag="PK264_00165"
     CDS             33822..34907
                     /locus_tag="PK264_00165"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD74342.1"
                     /translation="MAKKSAALLGLVVAVAASALTAFAVTQLNDPRRQDRDKAAPPPR
                     ALWAAAAPGRIEPKGGEFRIGPQSSGRIVDVVVQLNDRVVAGELLARLDDEELLTRMA
                     AMETENAVRKRERDAETVNRLALDRRNAEDALSNSERAVAKALHEVDRIRTSTKAGSA
                     AADALIAAQDRLAAARNKLLADRDALARLQATQGMPLPTRLEANLSAGRADLSLLETA
                     LERLRVRAPAAGSVLQLHAKVGETVAPSIEHPLLVLGDLTALRVRAEVEEREAPKIRI
                     GQKVVVRSDAHPGRDFEGQVVVIAQSLSAPKLGARGPRKPTDVDVLEVQVELAGGGPL
                     LPGMRVDVLFKPDATVATEPSPKASSN"
     gene            34965..35600
                     /locus_tag="PK264_00170"
     CDS             34965..35600
                     /locus_tag="PK264_00170"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HRD74343.1"
                     /translation="MTAESRIREQIVATGASLFNRGLTHGSTGNISVALPGGGWLMTP
                     TGSSLGALDPARLSRLDTVGRHIAGDPPTKEAILHTLMYARRTGTGAVVHLHSTHSVA
                     VSCLQGLDPANALPPITAYYVMRIGALALAPYFPPGDPKLAEAVADLADKHHAILLAN
                     HGPVVAGNSLAAATDAIEELEETARLFLMLRREVITPLTAEQVAELRKRFG"
     gene            complement(35597..37417)
                     /locus_tag="PK264_00175"
     CDS             complement(35597..37417)
                     /locus_tag="PK264_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74344.1"
                     /translation="MSGDGELKAAATAPLGLDPKTRSLLKRVLGDWVWPRRGALALSL
                     LAMVALAAVTGLYPQVIKYSFDALTKDQLGLMPQIVAMIIAITTTRSILLYLTNVLTN
                     RIVMRIGVAMQKRALGQLLKADYARLTRDSPGDLVSRLTNDVALIQQALQAGLNTALR
                     DALMVTGLVIYMIWRDPLLSLIVVAVYPLAILPIIAIGKRLKRVAKRTQAELGDMTAQ
                     LSEKLSGVRLIKTFRLEEYAAERMHGSFEQIYALRMKAVRNRAQLDPILEALGGIAVA
                     GVIALATWRIASGIGSIGDFMAFVTALVLAAQPTRGLGNLNAKVQEGLAAAERIYAII
                     DDEPKIVDRPGAKPLAVAGGAIRLDDVSFTYPGPQGEGERAPRAAVRGVDLSIPAGSS
                     AALVGRSGSGKSTILNLVPRLFDVSAGRILIDGQDVRDVTLASLRDAISLVSQDVTLF
                     DDTIRANIALGRLGAGEAEIVAAAKAAAAHEFILEQPAGYETVIGDRGMRLSGGQRQR
                     LALARAILKDAPILLLDEATSALDTESERLVQEALARFTKGRTTLVIAHRLSTVQASD
                     MICVMEDGRIIETGRHGDLIAADGAYARLVRSQSLGGEAG"
     gene            complement(37689..39782)
                     /locus_tag="PK264_00180"
     CDS             complement(37689..39782)
                     /locus_tag="PK264_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD74345.1"
                     /translation="MGAGALRLAVDVGGTFTDVVLEASDKGAATKVLTTPDAPERAIL
                     EGARHVLAELGRSFADIDVLIHGTTLATNAIIERKGARVALLTTEGFRDVIEIADESR
                     FDQYDLMIEKPKPLVPRRLRFGVPERLDVTGKVRLALDERRLEAIVASLAAERIEAVA
                     VAYLHSYVSAIHEERTRDVLAKRLPGLSVTLSSEVCPEMREWERTSTAVANAYVQPLM
                     SGYLGRLERSLAEAGYRRPIHLITSGGSLTSLETAQRFPVRLVESGPAGGAILSAAIA
                     AELGEKKVLSFDMGGTTAKVCLITDYEPMKARTFEVDRAARFLKGSGLPVRVPVIEMV
                     EIGAGGGSIARVDTLRRITVGPDSAASVPGPACYGRGGTEATVTDADVVLGRIDVAGF
                     AGGKLTLKPANAGTAVAASVGAPLGLSVEAAAHGIAEIVDENMSNAARVHAVERGVII
                     AEHTLIAFGGAAPLHAARMAEKLGIRRVVVPEGAGVGSAIGFLRAPAAYELVRSRFMR
                     LGELDLVMANDLLGAMSREARGLAEAAALGRPLEEQRVAYMRYTGQGHEIAVPLPNRG
                     IVAGDAAAIREAFEAGYRQLFERHIPGAEIEILSWSVLVSTPAGSPIPRSVPAAASRS
                     AGALEATGTVQAYDQRQGRRLAIPTYRRDAIGDGQSVDGPALILEAGTSTFVSALFAA
                     RRGTGGALVLERKGG"
     gene            complement(39794..40651)
                     /locus_tag="PK264_00185"
     CDS             complement(39794..40651)
                     /locus_tag="PK264_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HRD74346.1"
                     /translation="MPIDPEKLRSWKIADVQQTYTERDTMLYALGLGCGADPLDERDL
                     TFVYEDGLKALPTMAAVLGSPGFWVKNPETGVDWKKVVHGEQSVTLLKPIPKAATIIG
                     RTRITGLIDKGRDKGALLLSEREVIDKTSGTTLARLQSTSFLRGDGGFGNSTGTAPEI
                     HALPTRKPDEQRDMRTLPQAALIYRLSGDYNPLHADPKVGAAAGFRAPILHGLCSFGI
                     AGRAVLAACCGDDPARLTHMQVRFSAPIYPGETIRTEIWRDGATISFRARCLERDVVI
                     LNNGMARVA"
     gene            40923..41375
                     /locus_tag="PK264_00190"
     CDS             40923..41375
                     /locus_tag="PK264_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74347.1"
                     /translation="MDGFMKMFGHVANILKGYAIAIGVAVAIAFVWMWWKQRQEEART
                     ERQVRIQSLFAEQARLALAHLDIAKPRLVAQMSPEETTRYHWYLMEVVAINGQIARLD
                     NEGQWRSAIVDRLRVHREALSSKEFELLSHSSVSSELRQLIKQLEQRH"
     assembly_gap    41682..41701
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41702..41991
                     /locus_tag="PK264_00195"
     CDS             <41702..41991
                     /locus_tag="PK264_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74348.1"
                     /translation="IAGLAMLVAGGTATVYAAGGPGTQINDGQSLIAAFKCLADGNAT
                     CESFARAAAAAAKHNWQIAALIAGVGALWIGTAIRIATELRAMRVPPTRQR"
     gene            42297..44402
                     /gene="nuoG"
                     /locus_tag="PK264_00200"
     CDS             42297..44402
                     /gene="nuoG"
                     /locus_tag="PK264_00200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRD74349.1"
                     /translation="MPKQLIIDGKPIEVEDHLTLLQACEQAGADIPRFCYHERLSIAG
                     NCRMCLVEVVGIPKPQASCAMGVNDMPPRRDGKPHEINTRSAMVKKAREGVMEFLLIN
                     HPLDCPICDQAGECDLQDQAMAFGKGGSRHVEPKRAVEDKHLGPLIKTMMTRCIHCTR
                     CVRFMTEVAGVEELGAIGRGEDMEITTYLERGIMSELSANVVDLCPVGALTHRPWAFA
                     ARPWEMRKTESIDVMDAVGSAIRIDARGAEVMRILPRNNDAVNEEWISDKARHVADGL
                     RTQRLDQPYIRKNGKLVPATWDEALAAVAERLTAATPDRIGVIAGDLAGAEEMFALKD
                     LVTRLGVTSIDCRQDRSELDPALGRASYTFGAGIEAIEQADAILLVGTNPRLEAPVLN
                     QRIRKRWRQGALKVGVIGQQSDLTYQTTYLGAGPQTLGELVDGKHAFREVLDKAAAPL
                     VIVGAGAVSRSDGRAVLSAAARLATSVSADKPATWRTFNVLHTAASRVAGLDLGFVPT
                     GSGLSTAHMLTAAERGALDVVYLLGADELDMARLGKAFVIYHGTHGDAGAHRADVILP
                     AAAYTEKSATYVNTEGRPQQTAKAAFAPGDAREDWTIIRALSARIGKMLPYDTLEALR
                     ANMYLNVPALARIGAIEAGTADLSRAAVVGGPLSSEPFAPVIRDYYLTNPIARASAVM
                     ADLSALKASMTEEATGTNG"
     gene            44395..45489
                     /gene="nuoH"
                     /locus_tag="PK264_00205"
     CDS             44395..45489
                     /gene="nuoH"
                     /locus_tag="PK264_00205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRD74350.1"
                     /translation="MANIVWADIWRDYGWPLLLMVVQSLVMLASVLVILAFLLLFDRK
                     VWAAVQLRRGPNVVGPFGLLQSFADLLKFVFKEPLIPAGADKGVFLLAPLVTAVCALA
                     AWAVIPVSEGNFFGLASGKWVIADLNVGILYLFAISSLGVYGIIMGGWASNSKYPFLG
                     SLRSAAQMVSYEVSIGFVIITVLLVAGSLNMSEIVNAQRSGLGTKMGLPSSALDWYWL
                     ALLPMFVVFFISALAETNRPPFDLPEAESELVAGFMVEYSSTPYLLFMLGEYVAICTM
                     CAMTTILFLGGWLPPVNHWLFNWVPGVFWFLAKVVFVFFMFAMVKAMVPRYRYDQLMR
                     LGWKVFLPLSLVMVVIVAGALQYGPQLIGK"
     gene            45493..45675
                     /locus_tag="PK264_00210"
     CDS             45493..45675
                     /locus_tag="PK264_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74351.1"
                     /translation="MDLNKIFGTCLIVLGLIDVFILPRFMRHGPPIITSMVRLIGLGF
                     MAAGVVLVLGFVKFLS"
     gene            45727..46215
                     /gene="nuoI"
                     /locus_tag="PK264_00215"
     CDS             45727..46215
                     /gene="nuoI"
                     /locus_tag="PK264_00215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRD74352.1"
                     /translation="MNIAAGIKSLFLTEFVSAFFLTMRYFFKPKATLNYPFEKGPLSP
                     RFRGEHVLRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMTKC
                     IYCGLCQEACPVEAIVEGPNFEFATETREELFYSKERLLANGDRWEREIAKNLAIDAP
                     YR"
     gene            46258..46566
                     /locus_tag="PK264_00220"
     CDS             46258..46566
                     /locus_tag="PK264_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74353.1"
                     /translation="MTIAKRLTDAVFSVAQAEARASVWKEIVESDLATKRDFMEVEAG
                     LKRDIKELDTSLRGEIVQSVLRTRAQISELQATTLRVAMAMTAFTITAIGLMIHFLGR
                     "
     gene            46602..47225
                     /locus_tag="PK264_00225"
     CDS             46602..47225
                     /locus_tag="PK264_00225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRD74354.1"
                     /translation="MTLAPFFFYLFAATTVVAGLMVITARNPVHAVLWLILAFFNAAG
                     LFVLLGAEFLAMILVVVYVGAVAVLFLFVVMMLDVDFAELRAGFMKNAPLGLLIGAAV
                     LLELILVVGAWALKPTAGLVVRAPAPDIAKISNTRAVGQLLYTEPYVYLFQAAGLVLL
                     VAMIGAIVLTLRHKEGVRRQSIAAQNARTKAAAMTIVKVEPGQAQIP"
     gene            47286..48434
                     /locus_tag="PK264_00230"
     CDS             47286..48434
                     /locus_tag="PK264_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD74355.1"
                     /translation="MAEALACQRALFDIPDDVAYINCAYLSPLMRAVADAGHQGVDRK
                     VHPWTITRREFYAELDAVRGGFARLIGATADDIAIVPSSSYAAATAAANLPLASGQTV
                     VVIAGEHGSNVRIWHHLARDAGASITTVARPADDDWTSAVLAAISPATGIVALPPLHW
                     TDGSTLDLARIGTRARELGAAFVVDATQAVGAVPIDVAELKPDFLMCSAYKWLLSPYS
                     IGFLYAAPHRQAGRPLEQHSFNRAGAEQSEGSTDYPEAFEPGARRYDVGERSNFIALP
                     MLKVALDQLNAWGPARIAASTRPITERIVSEAAKLGLRAPAAAFRSPHIVGLTRPGKT
                     FPADLGQRLAARKVYVSIRGPAIRISPHVYNNAADVERLIALLAREAG"
     gene            complement(48531..48920)
                     /locus_tag="PK264_00235"
     CDS             complement(48531..48920)
                     /locus_tag="PK264_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD74356.1"
                     /translation="MSVEEQRNKGRAILTEVLGSAYMAKRDASTTDFNRPLRQFSETY
                     AFGDIWARPGLPRKVRSMLCMAMLTAMGKPAELELHVNSAINNGCTLEEIQEVLYQTV
                     AYCGIPATIEAFKVAEKVLKERGLLKT"
     gene            complement(48969..50033)
                     /locus_tag="PK264_00240"
     CDS             complement(48969..50033)
                     /locus_tag="PK264_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HRD74357.1"
                     /translation="MAAGIAAARATHHGARGIIQAWLWLTAALVFAMVIVGGATRLTG
                     SGLSITEWRPIMGIIPPLSDEAWQAAFALYKEIPQYKLVNKGMSLDAFKAIFWWEWGH
                     RALGRAIGIVFALPLAWFWWTGRIDRVLGLKLLGILALGGLQAVAGWYMVASGLVELV
                     SVSQYRLAVHLSIAFVILGALVWVALDLARDGRQGSRDGGGARAAWIFAASLTALLLV
                     QVIAGAFVAGMKAGLLYNTWPLMEGRLVPAGLFAKSPWWVNLGENMLTVQFHHRLLAY
                     TLLVASWGHAVLEMRRDGTSRIALSALFLALALTAQAAFGIWTLLAGVPLSLGLVHQG
                     FAAVVFALAVWHLHRARAAS"
     gene            complement(50293..>51239)
                     /locus_tag="PK264_00245"
     CDS             complement(50293..>51239)
                     /locus_tag="PK264_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD74358.1"
                     /translation="LWTLPMFHCNGWCFPWSISTVAGTHVCLRWVRAKAMFDAFADHK
                     VTHLCGAPIVMSTLLNAPPDERRPLPHRVEFMTAAAPPPEAVLAAMGDAGFNVTHLYG
                     LTESYGPAVINDWKSDWDKLAADARATMKARQGVRYQALEDLTVMDPETMQRVPADGQ
                     TIGEVMMRGNIIMRGYLKNKAATEEAFAGGWFHTGDLGVLHADGYIQLKDRSKDIIIS
                     GGENISSIEVEDVLYKHPAVSAAAVVAKPDEKWGETPCAFVELKPGIKAEPAEIIDYC
                     REHLARYKCPRHVVFTEIPKTSTGKIQKFKLRELAKAV"
     assembly_gap    51240..51249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<51250..51930)
                     /locus_tag="PK264_00250"
     CDS             complement(<51250..51930)
                     /locus_tag="PK264_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD74359.1"
                     /translation="MSMNPYEVDLGKTPANYQPLTPISFLARAALVFPEHTAVIHGEQ
                     RFAYRELYARSRRLASALAQRGIGPGDTVSVMLSNTPPMLEAHYGVPMTGAVLHSLNT
                     RLDAAIIGFQLDHANSRVLITDREFSKTIKEALMHCRVTPLIIDYDDPEFPQTGDRLG
                     VFGYEEFIGRGDPDFAWRWPADEWEAISLNYTSGTTGNPKGVVYSHRGAHQMGYANVV
                     AAGLGKHPV"
     gene            complement(52407..54227)
                     /locus_tag="PK264_00255"
     CDS             complement(52407..54227)
                     /locus_tag="PK264_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HRD74360.1"
                     /translation="MMRLQVIAGLVASLATVMVAVTWQSGHGQTPATKTPATKTPATK
                     APATKPAAGPSGSVTLIHIGDIHGHLVPRPNLRRDASAKTAGGVARMHTRIEEIRKRR
                     RNTLLVNTGDTIQGSAEALFTRGQAMVDVLNLLKIDVFAPGNWDFVYGPARFIEFFGE
                     GTPKAPWGTVAANVYFDGEPYADRTGKTVVPPYRIRQIGALKIGIMGLTTRRGPQVVG
                     KNVTKGFKFTSGEEEVPRLLKELREQENVHLVVLLSEQELANNIRLAEKNPGINVILS
                     SDMHEVTRRPIETSTGTIIVEEGQDGTMLGELTLEVDGGKLRSWSWTPHLIDESIAES
                     RVVAAKVAEVRKSFVTGPAFTKHKNPFNGTELARPIDTVVGETKVALHRSGFTDDAAP
                     AVIEGSSHNFLTDAFRTVAKADIGAIRGFRYGTHVAPGPIRMEDLYHYIPIGPLIAKG
                     TIKGQQLKNQLENSAEGALSTDVASWTGGWLFNFSGVTADIDPGAGKGKRASAIMVSR
                     ADGSGPMPLDPAASYTYASYYYAADPTLINVVPSTEIAILKDDAGNPLDGVEVVVRYL
                     QSLPGRSVAAMPTRLRLKAPLPKPAYGNPEVQPLRGVPAR"
     gene            complement(54651..>54970)
                     /locus_tag="PK264_00260"
     CDS             complement(54651..>54970)
                     /locus_tag="PK264_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74361.1"
                     /translation="RPDGVGEDARIGPQRHARIDERAAADATADDNMHVLAQADIEEG
                     AGATDVHAPSEDAHLGAQFRKARGEVARAELATALDYGNALARTREAATPPPYPEPTT
                     MAS"
BASE COUNT         9231 a        18419 c        17743 g         9264 t
ORIGIN      
        1 acctggcgcg gcttgaggac atgatccgac ttcttgtccc cctcgatcac cgtgatcttg
       61 tgatccttgt catcctcgtt ggtgatcgcg accgaggcca gcaccggtgt ggcgagaagc
      121 atggccaacg tcacgccggc tgcaaggatc atcggtttca tggaacggga cctcgcggac
      181 acttcgggcg actggcgcca cccggcagaa gcggattggt attcgtgatg acgagttgca
      241 gataggggct cggcggcagc cccgaccaga cagtcgacgc tgctgccagt tatcgctgta
      301 acgtccggct tggcaatcca ttccagtcgc atcgcgtcat gccgcggtgc gacctatcgg
      361 aggttcccgc agaaccgctg gatgcgcttg caggcatcct cgagctcgtc ggtcgagacc
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc cgtgcaccgc
      481 cgcgacgcct tcttcctcga gcagcgcggt gacgaaatcc tcgtcgttct tgatcacctt
      541 gccgctcttc gaggtcttgc cgatcgtgcc agcacacgag ggatagacgt agaacgcgcc
      601 ctccggcttc ggacacttga ggcccttcgc ctggttcagc atcgagacga cgagatcgcg
      661 ccgctccttg aagatcgcgt tgttgcgcgg aatgaagtcc tgcgggccgt tgagcgcctc
      721 gacgcccgcc cactgcgtga tcgacgaggg gttcgacgtc gactgcgact gcagcttgca
      781 catcgccttg atgagcggct cgggaccgcc tgcatagccg agccgccagc cggtcatgca
      841 gtaggccttg gagaggccgt tcatggtgag cgtgcgctcg tagagcttgg gctcgacctc
      901 ggccggcgtg acgaacttca gcccgtcgta gagcaggtgc tcgtacatgt cgtcggtgag
      961 cacccagacg tgcgggtggc gcagcagcac gtcggtcagc gccttgagct cagcgcgcga
     1021 gtaggcggcg cccgacgggt tcgagggcgc gttgaacagg aaccacttgg tcttcggcgt
     1081 gatcgccttc tcgagcgtgt cgggcttcag cttgtagccg tcctcgaggc gcgtcatggc
     1141 gaacactggc ttgccgtcgc cgagcatgac gatgtcggca taggagaccc agcacggcgc
     1201 cggtatcacc acctcgtcgc ccggattgat ggtgcacatc aacgcgttgt agagcacctg
     1261 cttgccgccg gtgccaaccg taatctggct ccgcgtatag gtgaggttgt tctcgcgctt
     1321 gaacttggcg acgatggcgt ccttcagttc cgggatgccg tcgacgtccg tgtagcgcgt
     1381 cttgccggaa gcgatcgcct tgacggccgc gtccttgatg ttctgcggcg tttcgaaatc
     1441 cggctcgccc tgcgagagcg tgatgatgtc ccggcccttg gctttgagct cgcgcgcctt
     1501 ggtggagatg gcgatggtcg ccgacggctg gatgcgattg aggctgtctg cgaaaaagcc
     1561 catgacgggc cctcctcctg tcggttacga gcggccggga ccgtagaggg tgaggcgaca
     1621 cttcgcaagg agactccgca agcagcccgc cgctcgcaca tgtcgctcgg acacgccgcc
     1681 gttgcgcgca cgagtgctct gtgggacgct caattggacg atacgctcag accggagctc
     1741 caacgatggc cgcttcccgc cccttactcg ccctcgccat gggcgatccc gccgggatca
     1801 gccccgagct cacggcgaaa ctcctcgccg acgaggccgt ggcggctgcg gccgacctcg
     1861 tcgtcattgg cgaccggcgc gtgcttgcgg ccggcgaaag cgttgccggc gtcaaggccc
     1921 gcactgagcc ctggatcccg agaaaggctg tcggcggagg gcacacattc gtcgatctgg
     1981 cacacctgga tcccacgcgg attgcacgcg gcgcctcttc gaaggagggt ggcgccttcg
     2041 cgctccgcaa tttccgcacg gcactggcgc tcggtcggga cggcgtcgtc gacgccgtga
     2101 cgtttacgcc attcaacaag cacgccatga agctcgcggc gggcagctac gaggacgaga
     2161 tcgccgtcat cagcgaggtg accggcacca agggccacgg ccgcgagttc aacatcctgg
     2221 gtgcgctctg gaacgctcgc gtgacgagcc acgtaccgct caaggacgtc gccaagtaca
     2281 tcacccggga ggcggtggta aacgggctcg tcctcaccga tcgctgcatg aaggaagcag
     2341 gtgtcgcgcg gccacgcatc gcggttgccg ggctcaaccc gcacgcggga gacgaaggca
     2401 acttcggccg cgaagagatc gacgtcatcg cgccggcagt tgtcgaagca aaggcgaaag
     2461 gcatcgcctg cgagggcccg ttcccatcgg ataccgtgtt cctcagggcc aagcgcggcg
     2521 acttcgacgc cgtgcagacg atgtatcacg accagggcca gatcgcgatg aagctcatgg
     2581 gcttcgaaag cggcatcacg ctgatcggcg gctatgcctt cccggtctgc acgcccgcgc
     2641 acggcaccgc ctacgacatc gccggcaagg gcatcgcgga cttgggcgcc agtcgcaacg
     2701 cgatcttgat cgcggcaaag atggtatcgc agcgcaaggg cgcgccggcg agcagcagtg
     2761 atcgggcagc cgccctcgcg cgtagtctcg acgcggccaa ggggcaggcc gcctgatccg
     2821 ggcgaggtaa gcgatgtcga ctgcagcaca gccggattcc cccggaatcg cactcgtaac
     2881 cggcgctgcg catcggatcg gccgggcaat cgcactccac ctcggccgcc tcggctggtc
     2941 ggtcgccgtc cacttcaacg gctcgcgcga ggccgctgag gctgtcgcag ccgaggtcag
     3001 caaatgcggc gggcgcagca tcgccttgaa ggccgacctc gcctcggccg acgaggtgcg
     3061 cgacctcctc ccgcgctgcg ttgcggcgat aggcgccccg acatgcctcg tcaacaacgc
     3121 ctcgatgttc gaggacgacc gcatcgagag cctcgatgcc cggctatggg acgcgctcct
     3181 cgctgtgaac ctcacggcac ccgtgctgct ggcccaggcc ttccagcgcc acctgccggc
     3241 cggtcagcat nnnnnnnnnn cgcgatcgtc aacatcatcg accagcgcgt gtggcggccg
     3301 tcacccgagt tcttctccta cggcgtcacg aagtccgcgc tctatgcggt aacgcgcatg
     3361 ctggcgcagg cgatggcgcc gcgcatccgc gtcaacggca tcgggccggg gccggtcctg
     3421 aaatcgatcc accagagcga ggccgaattc agcgccgagt ggaagagcac gctcctcgcc
     3481 cgcccgacct caccggacga ggtcgcggct gccgtcgcct tcatcctctc gcagccctcg
     3541 atgacaggtc agatgatcgc cctcgacgcc ggccagcatc tctcgtactg agccgatgct
     3601 cccgctcgcg gccttcacga cgcatccctc gctcttctcg ctgttcgggc tcgtcgtcgg
     3661 ggatggtggc gcgcgggtcg agcagggcat cacctacggg cccgatgcgc gccacctcct
     3721 cgacatctgg cggccgcgac gcgcgcccga cgcggggccc gtcgtcgtct acttctatgg
     3781 cggcggatgg acctcgggct gccgcgccgg ctacggcttc gtcggtgggg ccatagcgtc
     3841 gatcggcgcg acgtgcgtca ttcccgacta ccggctctat ccgaaggtgc gctggcccgt
     3901 gttccaggag gacgcggcgg cggccgtcgc ctgggtcgca cgcaacgtgc ccggcatcga
     3961 gcgccgccca ctcgtcctca tgggccactc cgccggcgcg cacctcgccg ccatgcttgc
     4021 cctcgatccg cgctggctca gggcagcagg cgccaatcgt ccggtcgacg gcctgatcgg
     4081 cctctcgggg ccctactggt tcgagccgac cacgtggccg acgaccgctc atatctttgc
     4141 ggacgccaca tcaaaggacg agccgcgacc ggtgtttcat gcgggccctc acgccccgcc
     4201 cgcgctcctt gtccacggcg aggccgacga cctcgtgaag ccgcacaaca cccacgagct
     4261 tgctgcggcg ctcgaggcgg cgggacggcc cgtgacaaag ctggtggtac ccggtgccgg
     4321 acaccgcaag accctgctcg gcattgccag gccggtcagg tggcggacgg atgcccgcgg
     4381 gccgattgcc gaattcttgc gcggaatcga tggcaacggc attacttcac tacatgctac
     4441 ggacgcacgc tgatcgagac atatgtcagc gcgaacatcg tcggttcctt gcccgcgagc
     4501 gtcgtcacga tatgtctcgc gaggacaaac tcgatatccg ccgaccactg ctcctccagg
     4561 cgcagcgtca tgccatcgga atacctgatg aacttgttga tcgggatggt gacgtagcgg
     4621 cagccgccga tctccacctc ggacgtggta tcgccgatcg tcagccgcat cttcgcagtc
     4681 ttcgttggct cgtccgtccc gttgacaaga cttttcgacg ccatcgaatg cgaaaggttg
     4741 tgcgtcgtac ctgacttgag cgggaagatt gccgacaaat cctggtcgaa gagaacctgc
     4801 ctcctggtcg acgacgactc cgaaacgagg gcgatcaagc cgcgatagtg actgtagccg
     4861 atctgaggcc gcatgggctt ctcaaacctc tcaatggact ggacgatccc accatgtcct
     4921 ccgacaacct ccgtcacgac gccgtcatcg cgcttcagaa tgaagccctt ggcggcgctc
     4981 tccctggtcg ggcactgcgc cgtcgccgcc gatgccgcca ggattgagaa aaggatcgcc
     5041 agtggcaccc gcatctcttg ccccccgcct ctcgtccgac cgccccatca gggccgtgcc
     5101 gaaatcgccg tgaactccaa tgtcagtcgc acgggctcct tccccgcaag tcgcgagttg
     5161 acgaccgtgc ggaggacaag ctcgagctct tcagaccaga actcctgatt cacgtactcg
     5221 tggccgttgc tgaaggtgac ggccttggtg acggcgatcg ttggatagct gcacgggcca
     5281 atcgcgactg tcgatcgctc cgtcgcaatg ttgagggcga ggcgggccgt gatgctcggc
     5341 tgcccgtccg acgagatctt gtagccaagg tcgtgcgccg caccgggcaa tagcggaaag
     5401 atcgcgagcg ggctctggtc gtagttgacc gtggccaatt tcgccttcga ctttgacgtg
     5461 ttcggcagga gcccgcggat ataggacttc tcgattggga gatgcgaggg atcggggtat
     5521 cgctcgctca tcgagacgat atcgttgccg cgccccgtga cctcgagcgt cataccatcc
     5581 atgcgcgtca ggacgaagcc cttcggcgac gtggtccagg tcgggcaggc ggcttcagct
     5641 ccaccgacag gaagcgacac gatcatcaag gcagccagcc atcgcagcat cgaaacgcct
     5701 cctatcgctt tggccgccgc ggctttgcct tggtccggtc cggggcaccg tggatcggtg
     5761 ggaatacgcc ggtcgtcgag aaggtgccgc gtcgggcgac gggggctgct ccaggccgtt
     5821 ggcgcgaggg ctcgccggac ttccgcggca cgaagcccgc cggctgtccg gccggaatga
     5881 gatgctgctt ccccggcccg atgagatcgg cgcggcccat gcgcttcaag gcctcgcgca
     5941 ggagcggcca attctcggga tcatggtagc gcaggaacgc cttatggagg cggcgctgct
     6001 tcaggccctt caccgcctcg accttttcgg agccgccccg gcgcacgccg cgcaacggat
     6061 tgaggccggt gtgatacatc gcagtcgcag tcgccatcgg cgagggcagg aacgtctgca
     6121 cctggtcggc gcggaagccg ttcttcttca gccagaccgc gagcgaaagc atgtcctcgt
     6181 cgcgcgtgcc tgggtgcgcc gcgatgaaat acgggatcag atagtattcc ttgccggcat
     6241 ccttcgcggc ccgatcgaac agctccttga agcggtcgta agtgccgatg ccgggcttca
     6301 tcatcttggc gagcggcccg tcctcggtat gctcgggcgc gatcttcagg tagccgccga
     6361 cgtgatgcat gacgagttcg ttcacatact ccggactctc gacggcgagg tcgtagcgta
     6421 cgcccgaggc gaccatcacc ttcttgatgc cagggagctc acgggccttg cgatagagac
     6481 cgatcagggc atcgtggctc gtatcgaggt ttgggcagat gcctggatag acgcatgagg
     6541 gacgccggca gagcgcttcc acccgcttgt ccttgcaggc catgcgatac atgttcgccg
     6601 tcggtccgcc gatatccgag atgatgccgg tgaagccctt ggtcttgtcg cgaatctcct
     6661 cgatctcgcg cagcaccgaa tcctccgacc tgctctggat gatgcgcccc tcgtgctccg
     6721 tgatggagca gaacgtgcag ccgccgaaac agccgcgcat gatcgtcacc gagaagcgga
     6781 tcatctccca agcggggatc ttggcgtcac cgtaggaggg atgcggcccg cgcgcgtatg
     6841 ggaggccgta gacgccgtcc atctcctcgg tcgagagcgg gatcggcggc ggcgtgatcc
     6901 acagatcgcg gtcaccgtgg cgctgcacta ggccgcgcgc attccccggg ttgctctcgc
     6961 ggtggagtac gcgcgaggcg cgcgcgtagg cgtccttgtc gtcctgcacc tgctcgtagg
     7021 ccgggagccg cacgacggcg gggccgttcg cacgcgcagc gccctcatca gcgctttcga
     7081 catcgtctgc cgcgatctcc gtgaacccgt ccggcaggct gctcttcatg acagcgatgc
     7141 cgcggatgcc gtcgatcgcc gagagctcca cgccggctgc tgcgcgatgc gccacatcga
     7201 cgatcgcccg ctcggcgttg ccatagatga ggaggtcggc cttggcatcg gcgagaaccg
     7261 agcggcgcac cttctcggac caatagtcga aatgggcgat gcggcggagc gaggcctcga
     7321 tgccgccgat caccaccggg acgtcgcgga aggcctcgcg acagcgctgt gcataaacga
     7381 tgacggcgcg atccggccgc ttgccgctct cgccaccggg cgtataggcg tcgttggagc
     7441 ggatgcgccg gtccgaggtg tagcggttga ccatactgtc catgttgccg cccgtgaccc
     7501 cgaagaagag gcgcgggggg ccgaggcgca tgaagtcggc agggctccgc caatcgggct
     7561 gagcaatcat gccgacacgg aagccgtgcg cctcgagcag gcggccgatg atcgccatgc
     7621 cgaagctcgg gtggtcgatg taggcgtcac cggtgacgat gatgatgtcg caggcgtccc
     7681 agccgagcgt atccatctcg gcccgtgaca tggggaggaa cggcgcggcg ggagggcggc
     7741 tcttcggcgc atgctcgaag aggccgtgtt caggcgtcaa cggcgtgaag tcggcacgct
     7801 tcgccatcgc cggcaatacg ggggcggcag cagccggagt gggcaccggt gacgacttgc
     7861 ggttgtagac gacggccaaa gggtgtgctc gcgaatgaaa gcaggcggcg gcgatatgtg
     7921 cgcgccgctc aatagcattt tctatggtcg cgacacagcg cgaactcatg accgcagctc
     7981 gaccgcaaat tgcaggcgtc aaccgacctc cgacacagaa cggccggttt cgggggtaga
     8041 ttcgagtgca cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgc cagggtccat
     8281 cgttcgatca gaaggttctc tgggtctacc gccgcgccgg gctgccggtc gaagtgctgc
     8341 gcgagcacga gggctggctt cagatccgcg acagcgaggc cggcaccggc tgggtgctca
     8401 gctcgctcgt ctctgcgcgg cgaacggccc tggtcgcgcc ctgggaggtg aagcccggcg
     8461 tcgcgccgcc gcagatcgag atccgctccg agccgagcgc cgcctcgccg ctcgtcgccc
     8521 tcgtcgaggc cggcgtgatc gcgagcctcg tcagctgtga ccgccgctgg tgccggatcg
     8581 ccatcaacga catccgcggc ttcgtcgacc agaagcggct ctggggggtc tatgaggacg
     8641 aggtactgaa gtagcggctt cgattaaatt cgtgcccagg cgagtgcagc caatgcggcc
     8701 gctgcgccgg ccgtcaggag gagcccgcga cgtccatcga cgaagctgtc cggcatgggt
     8761 ttcatgacca gtggtgcgac gagccccatc gcgcagaacg caatcacggc aattgcgagc
     8821 ggcatgcgcc gccaagcaag cagcagtgcg agagcaagcg ctgcgaccgc aatggccatg
     8881 ctgccccaaa tgatcaccag gccgagcggg ctcgcgagcg tctcgcgccg cccttctgcc
     8941 ggaaaatgac cgctcgcgat aagcgcatag agcgtcaccg ccagcgacaa ggcgagaaag
     9001 agcgtcagct cgtagagcgg cggcagcata aaatgtcccg ctttctggtg tcggttgtca
     9061 cgcggcggcc gtcgccgcga tgatctcacc tgctggtcgt gccgcaccct tcgactggct
     9121 gatgtcgaag gcgatccgcg gccaggtgac atggacaacg gcgcccgtcg ccggtgccgc
     9181 gcgattggcg aacgcgaggt gcgcgcccga gcgctcgagc agcgacttgg cgatgaagaa
     9241 gccgagcccg agcccggcag cctccgagcc ctgcttctgc tgctggccct gcggccgggt
     9301 cgtgacgtag ggttcgccga gcgcctcgat cacgccgggc gcgaagcccg gaccatcgtc
     9361 agcgatgcgg acctcgacgt tcctgtcgct ccaacgcgcg tcgatggtca cctcgccgct
     9421 cgcgaagtcc acggcattct cgatgatgtt gccgagcccg tagatgagac cgggacggcg
     9481 ctcggcgatc ggctcgcgca tcgcgcccgg gccatcaacc gcggcggccg ttgcgatcgt
     9541 gatccgcttc acacccttgc ggtagggctc ggccgcctcc tgcaggagtt gcgtcagcgg
     9601 caaactcgcg tggtggggat cctgttcgtt cggcttgcgc gtcagcgtgc gcagaatctc
     9661 gcggcagcga tcagcctggg cacgcaatag cttgatatcc tcctcgatcg ccggctggcc
     9721 ggctgtctca cgctcgagct ccttcgtcac gagcacgatc gtcgagagcg gtgtcccgag
     9781 ctcgtgcgcg gccgcggcgg caagtccgtc gagcgcgtgc agacgctgct cgcgggcaag
     9841 tacatgctcg gtcgccgcaa gcgccgcagt catctgacgc gcctccttgg tgaggcgggc
     9901 agcatagatg ccgaggaaga tcatggccga gacaacggag gcgaagatcc cgcccttgta
     9961 gacccgcggc aggacaaacg cctcgcccgc cccccagggc agcggcaggt gccactcgac
    10021 gagcagggcg gccatgacga cggccaggag gccgagaacg atcgtattgc gcggcggcaa
    10081 tgtcgcagcc gaaacggtga cgggcgccac cagcatgaag acgaacgggt tctgcacgcc
    10141 gccggtcaga tagagcaatg ccgcgagccc gacgatatcg caggcaagca gcgccgaggc
    10201 gagcgaggca ccgagccgaa accgcatcgg gaacttgatg cgcaggtaga tgttgaccca
    10261 ggccgagagc gcgatcagca cgaagcagag cgcgatgggc agcgagtaac cgagcccgaa
    10321 gtagacgagc agcactgtcg ccgcctgccc ggcgacgccg aaccagcgca gcttgatgag
    10381 cgtctgcaat ctgagacggc tctcctggat cgtcatcgga tcactcccgg ccaacggcca
    10441 ccgcccgcgg cgcaacctac gctcataacg cgagccgagc cagaccatat ccgggaccga
    10501 ggaaggcggc agcccagaca aaggaggata cgatgctcgc cggcacgaac ttccagagcg
    10561 gcatgacgaa catgcccgcc acgaccggaa tgaacgggcg gagcagcccc aggaacttgc
    10621 ccccgagcac gctcagcgca ccccagtcgc ggatcatcac gcggctctgg atgagccact
    10681 ccggtcgctt cgagcacggc cagatggaga cggactgatc cttgaagacg cggcctatcg
    10741 cgtaggtgac gcagtcgccg aggattgcgc cgatcgaagc ccagagccat gtcgtccaga
    10801 acacgccgcc ggccgcgcca tgcaccgccc cgatgccgag aaagagcgcg ctcgacggca
    10861 gcagcaacga caagaccggg atgccctcgc cgaaaccgag gaagaagcag accgggcctg
    10921 cgagccagtc atgctcgcgc acgagcgcga ccagccactg cttcagggtc tcgaatgaca
    10981 tgccagctca ggccctgcca tacgccgatc tcgcggcgct ggtcgaaact ttgccgtcgg
    11041 cgacgtcgcg ctcgagcatg gcgggatcgc gcagcctcgg ttcgccgtag cctccggcac
    11101 cgggcgtcac aatctcgacg ctcgagccct tggccaggca tgcgcggccc cgatccggag
    11161 cctcgctcgg agcattgaaa ttgaagtgcg cggtgcctcc acgatgcccg ccatcgaggc
    11221 cccagggcgc gacggtcgcg cgcccgccga taatgtatgt ccggcattcg tcgtgctcgg
    11281 cgcggaccac gcggcggagt gccatgccgc cgcgcatgcg gcccggacca cccgagtcct
    11341 gcacgagctc gtagcgctcg atggtcagcg gatactccgt ctcgagcgct tccacgggca
    11401 agttcgaggt attggtgagg tgcacatgga cgccgtcgag gccatccttc gttgcccggg
    11461 ccccgaagcc gccgccgatc gtctcgaggt aaacgtagta gcggccgttg cgagggtcga
    11521 cgcccgagaa gtcggcatag acgcaagcgc cgttgtgagc cgccgtgaca cgctccggga
    11581 tagccgtggc gagcgcaccg tggatgagat cgacgacgcg ctggccggca tcgaggcgcg
    11641 agttcaccgc agccggcggc gccgcgttca gcaccgagcc cttcggcgcg gtcaccgtga
    11701 tcgggcggaa gaacccggcg ttcggcggga tgtccgggcc gatcacagtc ttcagcgcgt
    11761 agtagaccgt cgccagcagg cccgtctcga ccaggttgaa cccggcacgc acctgccgcg
    11821 gcgcatcgaa cgtcaaggcg atctcgtcgt ccttcacgac gatctcgacc ttgaaggtca
    11881 ggttgccgtc cacctcctca ctgtcgaaga cgtcctcgaa gcgataggtg cccgagggaa
    11941 ttgtgcggat gcctgcccgg gtgaggcgct cggcatagtc gagcagtgcc gccgccgccg
    12001 acatgagggt gtcgcggccg tagcgccggc agagcgcctg gaagcgctgc acgccgagcc
    12061 ggttcgccgc catctgcgcg cgcaggtcgt tcagccgctc gtcgggcacc tgcatgttga
    12121 ggagaatcat ctcctgcacg tccgtgacga gttcacctcg gcgatagagg cgcaccggtg
    12181 gaatgcggat gccttcctgg aagatatgag catggccgcg gtcgacgaag tcggcgtggt
    12241 gggccgtgtt ggtcgcccag ccgacgagtt ggccctcgaa gaagatcggc tcggcgagca
    12301 cgaggtcggg gagatgcgtg ccgccgcccg tataggcgtc gttgccgatg aacacgtcgc
    12361 cctcggcgat ctcggccatc ggcacgcgtc ggtagatcgc ttcgacgatg ccgatcagcg
    12421 agccgaggtg cattggaatg tgcatcgcct ggcagagggt gcggccggcc gcatcaaaga
    12481 ggcaggtcga gctgtcgcgg cgctccttga tgttggtcga ataggaggcg cgcacgagcg
    12541 tctcccccat ctcctccgcg atcgaggcga gcgcgttgcc gatcacctcg acggtgacgg
    12601 gatcgagagc cgccttgcct tcgcgaactg ccttggccat accgtcgcct cctggagtcg
    12661 agtctgcgca ttttgtcgat agatcatgaa gcgcatcagg gccgcaaggg cgatcgaggc
    12721 aaccaatccg aagcagctcg tggcgcagag gtcttgaaat ccaatggcct ttgcaaagtg
    12781 gcttgaaacc agaataatct gtggataaaa atgctgagcc agttcatgcc acagaaccgg
    12841 tgtagactga gggtgtggct agttgcggtc gatcgggggc gatcggctgg tgtgtgcgga
    12901 gcgtagcgtt ccatccggcg ggatgggttg cggggtagtc atgttagtca agtcgatggt
    12961 gctcgccgcc atggcgacgg caatcgggcc tgtgctcggc attgccctct ttgtcctcaa
    13021 acactcgctc tgacgagcaa ccgacgcgct gaccggcgct cgttgagtcg tgtcggcatg
    13081 ggccatgggc gcggttgcgc gaggtcgcat gcgcgcgacc atgcgttcgc ctgcgcatgg
    13141 gactgcgtaa ccgtgcgtgc tcgcgcgaga cgacgccagt tgcaaaaatc cgcaatggtg
    13201 gcgccacgca tagttgcgcc atgacgacgc tcgggcatag gcacggggca tcgacgggcg
    13261 tcgagggaag attcggggcc aacgcctcac cgccgacctg gttccccgga aatccgagca
    13321 cgcacttgcg tgcctgaaat gtccggggcc gttacccgct tgggcgtcac tgcaggcctc
    13381 aaagatcgcg acccgcgtcg tcgcggatgg catcggcgaa gttgtcccgc caggtcggag
    13441 tgtgcgccgc gatgctcgtg aacttccggt cgtggcacca gcgcatagtg taacgccgga
    13501 cagtctggtc actttggcgc gtgctcggat ttcctggaaa ccagcggaca gggttgggga
    13561 gcagtgcggc gtcaggccta aactgacacc ccttccgtca tcgcgccggc aagtcgagcg
    13621 tcgactcgcc gctacgtgcg ctcggccgct gtgcgtgcga aatgctttgg gcaaccggtc
    13681 tcagcattga cgtgggttca ccgggaccgt cgtggtaggg tctcagttca agctcgccca
    13741 attcggacca cacgcccgat ccacctcacc gggaacctca tgacattgcc gctgaaactg
    13801 ctgagccccc tcgacctcgg ccacacatct ctcgccaatc gcctgatcat gggctcgatg
    13861 cacacccgcc ttgagaccct ggaccgtccg ctcgatcgga tcgcccgctt ctatgcagag
    13921 agggcccgcg gcggcgccgg cctcatcgtc accggcggct tctcgccgaa cgacgacggc
    13981 ctgatcgagc cgaacggtcc ggtgctttcc agctcacatc agctcggcga gcaccgcccc
    14041 gtcacggatg ccgtgcacgc tgccggcggc aagatcgccc tccagatcct gcatgccggc
    14101 cgatacgcga cacacgaact cgcagtcgga ccttccgacg tcgcctcgcc cataaacccg
    14161 agaaagcccc gtcgcatgac ggaggcggac atcctgcgca ccatcgagga tttcgccacc
    14221 tgcgccgccc tcgcccgcga ggccggctac gacggcgtcg agctcatggg gagcgaaggc
    14281 tacctcatca acgagttcac ggcgccgcgc accaacgacc gcgtcgatgc ctggggcggc
    14341 agcctcgaca accggctgcg ctttccggtc gcagtcatgc gccgcgtacg cgaacgggtc
    14401 ggggcagatt tcatcgtcat gttccgcatc tcggccatcg acctcgtcga cggcggtcta
    14461 acgtccgaag agatcgttgc gcaggcgcgg ctcgtcgcca gcgccggcgc ctcgatcatc
    14521 aacaccggca tcggctggca cgaggcgcgc attccgacca tcgcgcagaa ggtaccgcgc
    14581 agtgcctggg gcttcgccgc acggcggatc aaggacgcgg tggccattcc cgtcgtcgta
    14641 tcgaaccgca tcaacacacc ggatgaggcc gagcgcattc tcacccgcgg cgacggcgac
    14701 ctcgtctcca tggcccgccc ctggcttgcc gacccgaaat tcggcctgaa ggcacgcgaa
    14761 ggccgcgcaa acgagatcaa cgtctgcatc gcctgcaacc aggcctgcct cgactacatc
    14821 ttctccgggc gcgtcgcgac ctgcctcgtc aatcctagag ccgcccgcga gctcgagttc
    14881 gacgccccgc cgccctccaa caggaagcgc gttgccgtga tcggcgcagg ccccgccggg
    14941 ctcgccttcg ccacaacggc cgcggagcgc gggcatgccg tcaccctcta cgaggccgag
    15001 gcacgcattg gcgggcaact caatctcgcc cgcacgattc ccggcaagca ggagttcgac
    15061 gagactgtcc gctattggcg caccctcatc gagaagcacg gcgtcgaggt gcgcctcggc
    15121 gcgcgcccga ccgccggcgc actttcggcc ggacgcttcg acgaagtcgt cgtcgcaacg
    15181 ggcgtgacgc cgcgccggct ggatatcccg ggtgccgacc acccctcggt cgcatcctat
    15241 gccaacattc tctcagggaa gcgcgtcgca ggccggcgtg tcgccatcat cggcgctggc
    15301 ggtatcggct tcgatgtcgc cgagctgctc tcctccccgc acgcgagcgg ggcactgtcg
    15361 gctgaaagct tcgccaccga gtggggcatc gatcgcagcg tcgcggggcg cggcgggttg
    15421 gcaacgcccg ccgaggagac gccgccgcgc gagatcgtca tgctgcagcg gaagacgggt
    15481 cgcatggggc gcagtctcgg cgtctcgacg ggctggatcc tccgcctcca gctcgccaag
    15541 cgaaaggtcg cgcaggtgac gggtgcagcc tacaggcgga tcgacgatca gggcctgcat
    15601 gtcaccgtcg gcggcgcaga gcgcgtgatc ccagccgaca cgatcgtcgt ctgcgccggc
    15661 caggagagcc agcgcagcct cgcggacgag ctcgtggtgc tcggtattgc gccgcacgtg
    15721 atcggcggtg ccgacgttgc cgccgagctc gatgcgatgc gcgcgatcga tcagggcatg
    15781 cgcctcgcct acgcgctgtg acgcgttgcc cgctcagaaa aggtgaacgg atttcggcac
    15841 cagcagcccg atcaggaaga agatcacgcc aacgcccgaa aagccggccg ccacccagaa
    15901 gagatagatt gccgtcgtga tcgcgaacgc cgaggcgagc acgatggcga tctggaaggc
    15961 cgccgacgac acctcgtagt ggtgatactt ggcgagtgcc agatcgcgct tcctttcggc
    16021 gtccttcgcc ttcgccatca gctcgacgct gccctcgccc gtctccggct cgctgcgata
    16081 gcgcgccgcg gtctttcgcc agtcaccgat gcgcttgtcg aggagctgct tcgtcgccgg
    16141 atcgcgcgcg agcttggcgt cgatctccat ggtctcggcc atggcctcga tcgtcgtgcg
    16201 tcggatggtc tttgcctgga agaacgccca ggtgtcggcc gccttgatgt tgtagccgag
    16261 cgcatccgtc tgcgcgctct tcgccatggt ttccgcgatg gcgaggcaaa gcgccaggat
    16321 cgagatgaag atcgcaatcg tcttgttgcc gccctccccg tgtccgtgtg ccccgtgtga
    16381 catgctccgc ccccatgagt tgctcagccc tctttggggc ggcagccacc atagagacga
    16441 gcggggcgca gagcgagacc cattccggcg atgagttgcg ccccgcctca aacctcaggc
    16501 atcaccttct cggcgcagag ccgcatggca cgcgtcacgt gagcggggtc gatgccgccg
    16561 atgttgaacc agagcgcgaa atccgtgatg ccgagccgcg acttgagatc gcggagctgg
    16621 gcaacacact ccttcggatc gccgactacg gccgcgccgt tctcgatgag cttgtcgaag
    16681 gcgaggtagc ccatctcgcg ggtcttcttg ccctcgctca tcgtgagctc gtagcccttg
    16741 acgacgccgg ccttcggcgc gccggaaagc tggttcgccg tcaccttcgc gtagaaccac
    16801 tcgacgtgcg gcttgtagat cgcgcgggcc ttcgcctggt cctcatggac gaagaagggg
    16861 agtagaatca tcttgcgcac cgtcttcgga tcgcgcccca tcccgcggca gacctcgtca
    16921 tgacgtgcga gcgagcgggc aagctcgtcg cgccaggtct cgcgataggt gcgataggtg
    16981 aagggcgacg ccatctgcag gttaaagccc gagcgggcag cctgctcgaa actctcgggg
    17041 ctgaccgttg cctggaacac cggcggatgc ggctgctgca caggctttgg cagcacctcg
    17101 accggctcgg ggatcgtcca gtagcggccc ttgtggctca ccttctcgtc tttccaggcg
    17161 cggagcacga tgtcgatgcc ctcgctcagc atctcgcggc tctcctccat cggcacgccg
    17221 aggccgacga actcgtgcgg ctggtaggcg cggccgatgc cgaaatcgag ccgcccgtgc
    17281 gagagcacgt cgacgagggc gaaatcggac gcggttcgca gcggatggtt gaacgggatc
    17341 accacgaccg ccatgccgat gcggatgcgc ttcgtgcgct gcgcgattgc cgcggcatag
    17401 acctgcgtcg aggtgacgac gccgtaggtc gagaaaaggt gctcggcgat ccagatttcg
    17461 tcgtagccga gggcctcggc gctctcgaag aacatcaagt cgttgtcgaa gctctcgacc
    17521 cgcttcgcct ggtcgggaaa ctggctcagg ctgaagatgc cgaaccgcgt gccgcttgtc
    17581 gaagccatgg ccaaactcgc tgttgtgcgc ttcctccatc gaagactgtt gtgatcggcg
    17641 cgaacagtca acggcttgca tcaatcgagg tgcgccatgc gacgaggggc tgctatgctc
    17701 gcggcccatc cggcaaaccc gtcgcgacgc agcaacaaag ccgcaccatg ccctgccccg
    17761 cattcctctc gcatccgatc ttcctcgcgt ccggattcgg ccggcatcat cccttgtcga
    17821 tcccgcgtca gtcgctggtg atcgggctcg ccgaggcgct cggctggctc gatcgggcag
    17881 acatcgaacc atgccccctc cccgacatcg gtgtgctcgc ccgactgcac gagccggcct
    17941 acctcgaggc tctcgagacg gcagcacggg ccggcgttgc ctcgcgcgag gcgcgcgagc
    18001 gctacaatct cggcacgatg gaatgcccga tcttcccggg gctctgggac cgggcgcggg
    18061 cgaccgtcgg cggcagcatc cgcgcggccg agctcgccat gtcggggcgc acggccttcc
    18121 accccgcggg cggaacgcac cacggcatga cggcgcgggc atccgggttc tgctacttca
    18181 acgaccccgc cttcgcggtt ctctcgttcc tcgatctcgg cgccgctcgc gtcgcataca
    18241 tcgatatcga cgcgcaccac ggcgacggtg tcgaggcgat gtttgccccc gacccgcgcg
    18301 tgatgacgct gtcgatccac gaggaggggc gctggcccgg cacgggtcgg ctcggcgatc
    18361 gcgcccgggg gcgcgcgcac aacatgccgg tcccgcgcga catctccgac agcgagttcc
    18421 tctacctgat cgacgaggct gtcctcccgc tcgccgagac cttcgcgccg ggcgcagtcg
    18481 tcgtcacctg cggtgtcgac acgctcgcgg gcgatccgct ctccggaatg gccctctcaa
    18541 acaccgccgt ctgggacgcg gtcgatcgca tcgcctgcct cggccggccg accgtgatcc
    18601 tcggcggcgg cggctataac ccttggacgc tcgcgcgtgc ctggaccggc gtgtgggggc
    18661 gcctctgcgg cgaggggacg gccatcgcgc tgccggaggc ggcgcgagca ctgctcgccc
    18721 ccttgtccgt cgacctcgtc gagccagagg atatcgatcc gcgctggctc tcacggctcg
    18781 aagacacccc gcatccgggc ccgatccgtg atccgatccg ccacatcgcc gagaaaatgc
    18841 gcgcgccttc acaatgaggc catggttgcg atcgacgaat ggcatgctgc tgcagtatct
    18901 tgcggcaaat tccgaagttt cgccgtcagg ggccggcgtt ccgggggttc cgtgatgcgt
    18961 ccaacgatct gggcgcgcga tacgctgatt cgtgcggtgt tctcagaggc ctatcgcctc
    19021 attgtgcccg gctatcagcg gccatacgcg tggccggccg atcatgccga acagctgctc
    19081 gacgacctcg tcgaggcgct cgagcggtcg aagtctgagg aaggcgcctc gagnnnnnnn
    19141 nnnnnnnncg gcacgatcgt gcttgcaaag gaaatccatc agccgctgtc gctaatcgtc
    19201 gacggccagc agcggctgat cacactgacg atcctgctcg cattgctgcg cgactggatg
    19261 ccgaccctcg gctccgtgac gctcgacgtc gataccgcga ttacctcggt gaaggacgag
    19321 ccgcgcctct caccccgtcc tgccgacgcc gacttcttcc gcgagtacgt gcaggacatg
    19381 ggggcgacgc gccggcaccc gccgaactgg gtgatgacgg aggtcggcct cgatgacgag
    19441 agcgaggcgc cggctgtcgc cagcggcgac gacgaggatc cctacgtcag tgatcccgcc
    19501 gccggagagc cgatggccga tgccctcgac gacatcgacc cgccacagcc aatcgattcc
    19561 gacgaggtcg tgatcgacgt gccgcacgtc ggcgaggagc cggtcatcca gggcatgcct
    19621 gccgcccacg acagccagcg tctcttcatc gagaaccgcg accggctgcg cgcgcgcctc
    19681 gaggcgctgg cgaagcagaa gcgcgtcaac ctcgccgatc ttgcctcctt catcctcgac
    19741 cgctgcatcg tcgtcgtcgt tgccgtccag cgcagcgacg aggcgcaccg cattttctct
    19801 gtcctcaaca cgcgcggcct cgatctctcg gacgccgaca ttctgaagtc ggaggtgctc
    19861 ggcgcgatca ccgatccggg tgcgcagcgt acctgcggcg cgatctggga atatgccgag
    19921 caggaactcg gccgcgacga gttccagagc ctgctcaccc acatccgcat gatcggcctc
    19981 aagcggcgac cgcgccgggc gatcgtggat gagctgcgcg atcactacaa gccggccgag
    20041 gcacccgtcg acttcgtcga gaacaccgtg cagccgatgg cggcgatcct cgccggcctc
    20101 aatgcggccg aggatgcagg caaggagatc tcgatccagg gcgccgacag cgccaccatg
    20161 gagcgcatcc gccgctacct catctatctc ggctggatcc cgaacgtcag ctggaagccg
    20221 ccggcgatcg ccttcttcat gcgctacggc gacaacccgt cggcgatcct gcgcttcctc
    20281 aagatgctcg accgcctctc gttcggcatg ctgatcctga agagcgacga gggcgagcgc
    20341 atctcacgct attcgcgtgt gatcgaggcc atcgaggccg gacagaacct cttctcgccg
    20401 aagtcctcgg ccatgaagct cgagcagcgt cagcgccaga tcatcctgaa ccgcatctcg
    20461 aatccctatt tcggcatcca gagctactgc ggcctcgtgc tgcgccgcct cgacgccgag
    20521 ctgatggtcg gtccgctgcc gagctatcgc gactcgaccg tcgagcacgt gctcccgcag
    20581 aacccgcgcc tcacgagcca atggctcgac tggtacccgg gccctcccga ggacccggcc
    20641 aagggcgtcg tcgagaactg ccagcggctc ggcaacctga tcctgctctc caagaccgac
    20701 aacaattgcg cagcgaacaa ccagctgagc atcaagctcg actgctattt cggggagaac
    20761 aatacctcgc ccttctccct gaaggacgag gtcgccagct tgcgctcctt cgaccagaag
    20821 gtgctcgatc gcctgcacga gcggcgcgtg cgccggatcc ggcacctgtg ggggctgctg
    20881 tagcgacgca ggccaggctc gctcgtcgac gcctggccgg atcacgcgat cgtcacgccg
    20941 ccgtcggcga cgatcacctg gccggtgatg aaggacgcag ccttcgaggc gaggaaggcc
    21001 gcgatgccgc cgatgtcgtc gggctcaccg atgcggcgca gcggggtcgt cgcgagccgc
    21061 gtcttgaggc gtgcctcgtc ctcccagagc gcacgggcga agtccgtctt cacgagcccc
    21121 ggcgcgatgc agttgcagcg gatgttcttc ggcccccact cgaccgcgag gctcctcgcg
    21181 agcgagaagt ccgccgcttt cgagatgccg taggcgccga gcatgctcga gccgcgaatg
    21241 ccgccgatcg aggaaacgat agtgatcgag ccgccgccgc gctcagccat gccgggtgct
    21301 gccaggttgg caagccagag gttcgacttg atgttggtcg ccatgatctt gtcgaactgg
    21361 tcgtcggcga tgcccgcgag cggcccgaag tagggattga ccgctgcatt gcagacgagc
    21421 gcgtcgagcc ggccgagctt gtcgagcgtc gccttgatga gcgcttcgac ttcgcccttg
    21481 cggccgatgt tgcaggggat gacgtgggcc tcgccgccgg ccttgcggat cgcgcccgcg
    21541 acctcctcgc aggcatccgc cttccggctc gagacgatga ccttggcgcc gaggttggcc
    21601 atcgtttcgg cgatcgagcg gccgatgccg cgcgaggagc cggtgacgag ggccacctgg
    21661 ccggtgagat cgaagggact tttcaaggca gcctcccgag gtgcgctggc atcgagagac
    21721 aataaggcgc gcggcggctc gaagacaacc gcgcctgccg acagcgacgc ctgcgctatg
    21781 aggtaagctg gcggcactcg tcgtcgtcgg gcagtggatc gcacttgcgg tcggcgacgc
    21841 aggcacgaag cgccgtcagc agcgctgcgg tgagcttgcc gtcgagatcg cccttgaagt
    21901 gccctgcatt gcgcatgccg cactgatagc gcttcaccca gatctcgccg ccgatccgca
    21961 tggcggtatg atagtcgtcg taggcgcgcg cgctccgccc gagcgcctga tagatgtggc
    22021 ctcgggtgtc gagccagggc gcggacggcg ccgcaacggc gagtgccagc tcgatgtcgg
    22081 cgaggccctc ttcgaacctc cctgacttgt agtaggccca ggctcggttg ttgcggatat
    22141 aggggttctt cgggtcgata cgcagcgcct cgccgtagtc ggcgaacgcg ccgggatagt
    22201 caccgaggcg gcggcgcgcg attgcccggt tgccatgaac gatgacccgc tcgccgtcgg
    22261 aaagcctgtc ctcgagcagc tcggtgcagg cggcaacacg agccttgaga tcggcaccct
    22321 ggcaggtctc ccagctgcgc ttgaaatcgc tgagggactg accgagggcg gcgctcactg
    22381 acaatgcgag gacggcgccg gcaagcagtg ccctgaggcc tcggtcgagc atggcaccct
    22441 cacatcgccc gcggccggca ctgctcgtcc tgtggaagcg gatcgcactt cttgtcgagg
    22501 acgcatttcg acaacgcctc gcgcagctcc ttgctgtcct tgccgtcctg ctgccccttg
    22561 tagaggccag cttcggcaag gccgcactgg tagagcttga tcatccgctc gccgccgagg
    22621 ttgagcgaca tctcgtaatc gcgcagcgcc cgcgccgtat cgccggccga ctggcggatg
    22681 tgggcacgcg tgtcgtaggc gtgcgggctc agcggattga ggcgaagcga cttctcgacg
    22741 tcggcgagcc cgagctccgg acggcctgag cggaaatagg cccaggcccg gttgttgagc
    22801 gcggtcgcgt agttgggcgc gatgtcgatc gcgctgttgt agtcgcggat cgcccgctcg
    22861 tagtcgccca tcaccgagta ggcgagcgcg cggttggcga aggcctgagc gatcacctcc
    22921 tgcggccagg ccggattggt gatcacctcc gtgcaggcat cgaccgcgcg cacggtgtcg
    22981 gcggtgaggt tgggattgag cagacactcc tcataagggc cggccgacgc cggaccggcg
    23041 gtgccgagga cgagggctac ggcgatggcg gcggcgcgaa acggtcgcat ctcaagtccc
    23101 cgactttgcc gccggacatc gcgcccgtcg gcgtcatact ctgccgcacc tgcgacaaga
    23161 ctgggtctca actaaggcag ttgccgtagc actgtcaggc gcggttgtcc ggcacctcga
    23221 tctccagacc gaggacgaga aagctccacg atttgccgtg gctgtcgagc ccgagcgaag
    23281 tattgacacc accttccaat gcgtccgcca gaacgaaatt caggccgtgc aggtgatcga
    23341 gctcgtatcg atcgacgcgg cccttgataa gtcctgggaa agccgccttc agccgatcgg
    23401 cggtgagttg cgccttgatc agcggataat cgcccgggtc gtaggcccag accgacacgt
    23461 tcgacgtgtt tcccttgtcg ccggcccgcg cgtgggcgat ctcgtgaagc ggtatttttc
    23521 gggtcttgcg gctcatgcga cgagcatccg tacgcttggc ttgaccgcac ctcgtgggat
    23581 cagcgcggaa tgcgtgacca cgctcggcgt gatctgaccg cgatagccgc cgccgccggc
    23641 cggaccacag caaagtaggc tctcggtctc ccagagcagc atctcgcagg ctgcccggtc
    23701 ggccgagcgc atggcggccc ggatgcggac atcggccggc atgctcgtgt ctggatcgtc
    23761 aatggcgagg tctgccgtcg cgtggagcgc attgacgccg acgagatcaa tccgcagtgg
    23821 tgccgatcct atctcgtcgg gcccgagccg ctctcgcaga atattggccg caagccgggc
    23881 gcgggcgacg gcgttgggcc cggcgtagct cacgcccgct tccgcataga agccgccgtc
    23941 gaagccgacc gtaaccttca gcgtctcagg ttgcgcccgt ccgctcgcgc ccgagacagc
    24001 gacccggttg gctccggcct cgccgacatt caccttggag aaatcggccg tgacgtcggg
    24061 cgtcaggtag gcgcgcgggt cgtgcacctc gtagaggagt tgctccttga cggtcctcgg
    24121 cgagaccatg ccaccggcgt ccgcgagctt cgtgatcacg gcttcgccgc cctcttcgac
    24181 ctcggcgatc ggatagccgc agcgcgcgag accgggcacg tccttccgtc cgggatcggc
    24241 gtagtagccg ccggtgatct gcatcccgca ttccatgagg tggccgacga gcgtgccgcg
    24301 gccgagccgc tcccagtcgt cgcgccgcca gccacagcgg tgcatgatcg gcgcgaggaa
    24361 cagcgaggga tcggacacgc gtcccgtgat cacgacgtcg gcgcctgcct ccagcgccgg
    24421 cagaatggca tcgacgccga gatagacatt ggcaccgaca accgcgagtc cgacctcgtc
    24481 cacgcgcttg tcgtgatggt cgaacagccg tgtctcgggg ccgatcatag ccgtgacgtc
    24541 gtctccctcg accgcggcga ccttcaggcc cgaaagtccg agctcgcgag cgatggcgac
    24601 cgtaagctcg gccgccgccg ccacgttggc gacgcccatg ttggtgacga cgcgggtgcc
    24661 attccgccgg caggccttga gcacgccgcg catgcgctgc ccgagctgcg ggttgtagcc
    24721 gcgccgcgga tcggctttcc ggtcgcgatg accgaacgcc atggtccgct cgccgatcgt
    24781 ctcgaagacg agatagtcga gccggccctt ctcagcgaga tcgatcgccg gaccgagccg
    24841 atccgaggag aacccggagc cacaacccaa tcgcaacatc cgggtactcc atcggtgacg
    24901 atctgcgagc gtggccgcgc cggcagaaca ggtcgcttac ttcgccggcc gcgacttcgc
    24961 ccactcgacg tacttcttcg agagcgcgta gttgtcgcga tagtacttgg tgtactcgtc
    25021 gccgatcatg accttgatgg cgaggccctg ctcctcgagc ttgctcacat ggtcgggatc
    25081 ggccatggcc gccttgaagg tgtcggcgag cgccttcacc acgtcggccg gcgtgccctt
    25141 cggtacggcg atgccgcgcg tcgaggacga gatcaccgtc ttgaagccga gctcgacgct
    25201 ggtcggcacg tcggggagga acttcgagcg ctgggtgtcg agcacggcga ggccgcggag
    25261 ctggccggcc ttcacctgct tgacgataga gccgacgttg tcgaaggcga cgtcgatatt
    25321 gccggcaagc acttccttga gctgggcagc gccgcccgcg aggtgcacca tgcggaagct
    25381 cgcgcccggc atggcttcct cggacatcag aatggcgagg tggtcatcgg agaggatgcc
    25441 ggtcgtcgcc gcgcggatgg tgccgggctt cgccttcgcc tcttcgagca gctgtttgaa
    25501 cgacttgaag ggactgtcgg cgcgcaccca gacgatgccg ggatcgagca cctggttgat
    25561 gaccggaacg aggctgtcga ggtcgtagat cgccttgcgc tcgggatcga ggacgacggt
    25621 gtgggtcgcc ggcaggttga cgtagccgat ggtgtagccg tcagccttcg cgcgtgagag
    25681 ttcggtccag ccgatctgtc cgccggcgcc gcccttgttg acgatcacga ctggctgctt
    25741 gaaggccttt tccgcgatcg ccccgacgat gcgggcgccg atgtcggtcg acccgccggc
    25801 cgggaaggcg acgatcaggt tgatgggccg gctcgggaag ccctgcgcga aggccacggt
    25861 tgcagctccc gcgatcaggc tcgcggcaag gcctaggctc gagagtttca ttcgcatcat
    25921 gtctcctccc aggtatgtct cgttggtcga gcggggccgc attggccacg cgcgacgtca
    25981 atcggccgtc gactcgcgca cctccttcgg ggcgagcgag agcgccgagc cgatgatgat
    26041 catagccgac attatgagca atgcgaggct cagccagcgc gtgaagaaaa tcgagaagtc
    26101 cccacccgac atctcgagcg aggtgcgcag attcttctcg agctccttcc cgaggatcag
    26161 cccgaggatc agcggtgccg ttggcaagtc gagcttgcgc atcgtgaagc cgatgatgcc
    26221 gaaggcgagc atcacccaga tgtcgaacac gctctggcgg aggctgtagg cgccgaggat
    26281 gcagaacagc agcgtgccga cgcagatgta ctgccaggga atgtgcagga tgcgcgccca
    26341 cagcccgacg aacggcaggt tcatcacgag caggatgacg ttcccgacga agaagctcgc
    26401 gatcaccgcc cagacgaggt cgggcttctc tgagaagaga aaggggcctg gcgccaaccc
    26461 tttcatgatg aaggcgccca tgatgatggc gatcgtcggc gaggacggga tgccgagcgt
    26521 cagcagcggg atcatggagg cattggcgta ggcgttgttg gcggtctcgg gggcagcgac
    26581 cccctcgatg gcgcccttgc cgaactgctc ggggtgcctc gagagcttct tctcgaggat
    26641 gtaggccata aaggtcggaa cgatcgcgcc aacacccgga atgagcccca tgaagagccc
    26701 aatgccggag ccgcggaaga tggcgccgat cgagcgcttc cactcggcgg ccgtcgggaa
    26761 cagacgggtg agatttgtct tgaggacgtc gcgataaggc ttctcggccg tgctcaggag
    26821 ctcgccgacc ccgaacaggc ccatgacgac aggaatgaaa tcaacgccgt cgtagagggc
    26881 gacgatatcg aaggtgaagc gcggcgcgcc tcgcgtcgga tcctggccga cgagcgacag
    26941 cagcaggccg acgatcgcca tcagcagccc ggcgacgatc gagcgaccgg cgagcgccgt
    27001 gacgaggcaa agcccgagca gcatcaggcc gaagaactct ggcgggccga acctcaaccc
    27061 gagctgcgcg agatagggtg ctacgacgac cagggcgaga gtcgcgatcg agccgccgat
    27121 gaacgagccg atcgccgcga tgccgagcgc ggtgccgccg cgcccctgtt tcgccatctc
    27181 gtagccgtcg aggcaggtca cgacggaggc agcctcgccc ggcacattga tcagcacgga
    27241 cgtgatcgtg ccgccgtaca tggcgccata gtagatggcg cacagcatga tgatgccggc
    27301 cgtcgcatcg agcttgaatg tgagcgggat caggatcgcg gtgccggccg cggggccgac
    27361 gccgggcaga acaccgatca aagtgccgag aaagcagccg gcgaccgcga acagcaggtt
    27421 ctgtccggtc gaagcggcga tcatgccgtt gagcagaaaa gtgagaccct ccatggctca
    27481 gatccctagc tcgccgagcg gcagcggcac cttgagccaa tggtagaaga catggaagac
    27541 gccccacgag ccggcgaccg cgagaaccgg gatagcgatc caattccgca ccccgaggag
    27601 cggcagcagc accgcgatga acacgagcgt cgccagccgg aatccaacat actcgacgct
    27661 ggccgcgacg agcgcgatgc cgacgaggat ggcaagcggg cgcggcacgt cggccagcgc
    27721 cggcagaacc ctcgcgtcgc cgtcgaggaa ggctccgccg cgcacgacct cggcgatcaa
    27781 gaacagggcc aggagcaccc cgaccccgcc gagccaaatg ccaaaaaagc cggggccggg
    27841 tccgagcgcg tcgagatagg cgtacttccg cgattcccac acggtgtagc cgaagagcgc
    27901 ggcgagcagc gccgacgcaa tgatccaggc acgacgcatg agaccctccc gacccctttt
    27961 cgggagagct tagcacgccg ttggcggccg gatagggctg aacgccgtca gcgcccccgc
    28021 atctcgtcgc gccgcatggg catggcatcg atggtcgaga acagcatacc ggctgcgagt
    28081 ggtttcgaag atgatatctt cgcgccgccg tccttgccga tgaggattgc ccggaagctg
    28141 ccgcgtgcca cgccataccg gcggcgcaag tccgccgcgg acagtccggg cccgccaccc
    28201 aactcgctcg aaacgctgtc gccgaccacc cagacgacca ccatgtgccg ctcggcgaag
    28261 ccgcccctcg ccccggcgac gatggcacgc tgcgaggcga gtgcggaggc gttgccatcc
    28321 ggcgcgaaca caacgagcgg ccgcttcttc cacatgtagc ccgacatggc gaaggccgcg
    28381 ctcggcgtga gcattatggc gagaagggct gtgatcaagg cagatctacg catcgctgca
    28441 ctcccaagtt gatctcgctt agcccggcag ccgtcgcttc gcgaagcggg gctcccgccc
    28501 cacgccccag ttggacggcc accctccaaa ccacccgctt ttcctcattc aaatagaact
    28561 tgctgggctc gagggcgccc cgcgcgcgcc agcgcgccag ctcgaaagca ccccgagcgg
    28621 gtctgggcgg cagcccgggc gcgcagcaag aacgcacggc gcctgctttg ggatcacatc
    28681 atcacggcgt cgcaaatttt gcgccgagcg tctccatcag cggacggaac tcgggaaagc
    28741 tcgttgcgac gaacgtaaca tcgtcgaccg ccaccgggcg atccgcgccg aggccaagga
    28801 tcaggaacga catggcgatg cggtgatcca tatgggtcgc gacgagcccg ccccccttca
    28861 cgccgcgccc gcccgtgacg agcaggctgt cgccatcgac cctggcgccg acgccgttcg
    28921 ccgcgagccc ggccgcggtt gccgcgagcc ggtcggtctc ctttaccttc aactccttca
    28981 ggccgtccat gcgtgtttcg ccgtcggcat aggccgcaag cgccgacagg accggatact
    29041 cgtcgatcat cgagggcgcg cgagcggccg gcacatgcac gcccttgagc ttggagtggc
    29101 gcacacggat atcggcgacc ggctccccgc actgctcgcg cctttcgagg atctggatgt
    29161 ctgcacccat ctcggcgatg gtcgtataga agccgacccg cgtctcgttg acgagcacgc
    29221 ctttgagcgt cacctcggag ccgggtgcga tcagcgccgc cgccgcgagg aacgcgccgg
    29281 aactcggatc gcccggcacg atgagatcgc gaccggcgag ttcggcatca ccccggacag
    29341 tgatcgccgt tttcccgccc cgctcaaccg tttcgaccgt gccaccgaaa tagcgcagca
    29401 tgcgctccga gtggtcgcgc gtcgcctcct tctcgacgac cgtcgtcgcc ccggccgcgt
    29461 ggatgccggc gagcaggatc gcggacttca cctgcgcgga cggcaccggc gactcatact
    29521 cgatcggcgc gaggctcgat gtgccgcgca cgacgagtgg catgcggtcc gacccctcga
    29581 ccacctcgag gcccatctgc ttcagcggtg tgagcacgcg ccccatcggc cgccgtgaga
    29641 gcgaggcatc gccgacgaac ctgacgcgca tgtcgtgccc ggcgatcaca ccgagcatga
    29701 gacgcgagcc cgtcccggag ttgccgaaat cgagcggtcc cgccggctcg gcaagcccgc
    29761 cgacgccacg ccccttcaca gtccaggatt tcccggactt cgcggcgggc gcaccgagcg
    29821 ccgtcaccgc cttcgcggtg ttgatcacat cttctgcttc gagcaggccc gtgatccgcg
    29881 tcgtcccagt cgcgagcgcg ccaagcatca gcgcgcgatg cgagatcgac ttgtcgccgg
    29941 gaagcgacac ggtgccgcca agggctctag cgcgcagcga ctgaaggggc gatggcgtgt
    30001 tggtcgcgtg ggtcacgagc gggctccgat aagctctcgc gccattgagc gggcgccgac
    30061 cggactgtca acgcttcgct taccgtcagt gccgaatgaa ccggttccgg agcatgccga
    30121 tgccggtgat ctcgatctcg acgacatcgc ccgagaccag gttgggcgag gcgccgtccg
    30181 tccccatcca gatgacgtcg ccgggatgga gtgtggcgat cgaggtgatc gccgcgatga
    30241 agtcggcgat cccgaaaatc atgtcattgg tcttgaagcg ctccgtctcg cgcccattga
    30301 ctcggcagat ggtgatgagg tcgtcgaggc gcacgtcggt ctcgatccag ggccccatcg
    30361 gcttgaaggt atcggaattc ttggcgcgcc agagcgtctt gtccgatttc tgccagctgc
    30421 gctccgagac gtcgttgccg atcgtgtaac cgagcacgca ggagagtgcg tcggccttcg
    30481 agagcccctt ggcgcgcttg ccgatcacga caacgagctc gccctcgtac tgcaccttct
    30541 cggtggcatg cgcagggatg atcacgtcct cgccatgggc gatcagcgcg ttctgggcgc
    30601 gatagccgac atcgggctgc gtcggcaatg taacggggag ccccatcttc ttggctgccg
    30661 ctgtcacgtg ctcggcatag ttgaggccga cgcaataaaa ggtctgcggc atcaccggca
    30721 actcgatctt cacgtcggcg aggggcacgc gccggccggt ccgcgcatgc tccgcaaagg
    30781 gcgagcccgt cacttccgct atgagctcat cctcgacgat gccgtagctc gtgacgcccg
    30841 caagcgaata gcgaatccag cgcatgggtc ctccgagatg gtggaggtac tatggcatgt
    30901 ccttatacag gtccgcagca ggcacgcgtt caacgcttcg gcggttgcgg tacgggagca
    30961 ggtgctgcgt tcgggaccgg ggccggcgaa accgcgggcg gcgggcaggc gaacatgcct
    31021 tcgggcgcgc gcttccagag gaacgtctcg ctcaggaact tcacgccgag atagccggtg
    31081 acctgcagcg tatcggcaga cttcaagcgg atctcaaggt catacttctc gcctttttcc
    31141 ggatcgtaga tccagccgta gtcccacacg cgcccgctct tcttgaggtc acccaggatc
    31201 tgcagcccgc aaatggtctg tccgcgcttg cccttcaccg ggttgttgag gtcacgcagc
    31261 ggctggccct tctcgtcgtt cggatccttc agccaggcga tatggccgca aagcttgtca
    31321 ccacagggcg caattgtgac ggcaccacgc cctgtgtggt cgatccacag tccgagctcg
    31381 ctcgggggcg cgactgcgct cggcgcagga ggcgacgcct tcgccgtctg cgccacggca
    31441 ctatactgcg aggtcgccat ggtgatgagg cctgcgaatg cagtcgccac aatcgcgccc
    31501 caagtcatta accatcgaga cattgttcac tacccctgtc attcaccgct cgatttctgc
    31561 ggcaacacgc ggatttcttc gcctctctgc ctctccttag agcgcttgtc gccgatatcg
    31621 ctttactttg acggtggtta ttcgggacaa gtgcctctat ataccgccac aacacggcca
    31681 tcccggggcg gcagagagcc gatacgcagc gcagcgcgcg ccgctcctcg cgtcaaccgg
    31741 ggcggatgtc aagcgtcacg ggataggccc tacggtgagt gcgtaaccgg aaacgtcatg
    31801 cgtagctcga aatgatcctg aaactcgcct tccgcaatct cgtgcatgac cgtatccggc
    31861 tcaccgtgac gctggtcggc atcctgttct cgatcgtgct cgtcgccgtg cagctcggcc
    31921 tctatctcgg cgcgcgcaag atgatcgtcg gcatgatcga gaatgcgaag gccgacatct
    31981 ggatcacgtc cttcggtgcc aagagcttcg aggaggcggg tcagctgacg ggccgcgagc
    32041 gttacggcgc gctctcgaca ccgggcgtcg atgccgtgac accgatcgtc gtctcgttcg
    32101 cggaatggcg caagcctgcc ggcggctcga cgctgatcgt gctcgtcggg accgatccgg
    32161 ccgacggcgg tctcgcgccc tggaacgtcg tcgatggcgt cggctcagac ctcacgacgc
    32221 ccgatgccgt gatcgcagac aggacctaca tcagggatct cggtgtcagc cggctcggcg
    32281 ataccgccca gatcgagagc ggcaaggtga agctcgccgc gttgacgagc ggcatccggt
    32341 cattcacgat ggcgccctac atcttcacga cgctaaatcg cagccgctcg ctgctcgcca
    32401 tgccgcagga ccaagcgacc ttcctgctcg tcaaggcctc tgatccggcg gatgtgccgg
    32461 cgctgcagtc gcgtctctca gcacgccttc ccggcagcga tgtcctgacc aaggcccagt
    32521 tcatcggacg cagcctcgac cactggctgt tcgcgaccgg tgccggcgtg gcactcatcg
    32581 gcggcgcgat cctcggtctc atcgtcggca cggtgatcgt cgcccagacg ctctactcca
    32641 gcaccaagga ccacttgaac gagttcgcaa ccttgcgcgc gctcggctcc tcgtccggct
    32701 acatccacaa ggtgatcctc tggcaggcgg ggctttcggc gctgctcggc tacctcatcg
    32761 gcatggccgt cgcgatggtg atcgtgcact tctcggagca gaccgcgctg ccgatcgtca
    32821 tgacaccctg gctcgccgcg ggcctgctcg cgctcaccgt cttcatgtgc gcgatctcgg
    32881 ccgtgtcagc gatcatgaag gtgacccgca tcgatcccgc catggtgttc gcccgatgag
    32941 tgcagtcctc gaggctcaag gcgtcgtcaa ggagctcggc cagggcgccg gcaagatccg
    33001 cgcgttgaag ggcgtggacc tgacgctcga ggcgggcgag cttacgctgc tcatgggccc
    33061 ctccggcagc ggcaagacaa cgctgctgtc gatcctcggc tgcattctct cgccgaccga
    33121 aggcacggtc gagatcggtg gcgagaacgc ggtcgggcgg acggccgagg acctcgctga
    33181 tatccgccgc cgccacatcg gcttcgtctt ccagcaatac aacctgttcc cgacgctgac
    33241 cgcccatgag aacgtgcgcc tcgcgctcga tgtgcgcggg gatcgcggca cggcggcgag
    33301 cgatcgggcc aaggaagcac tgaccaaggt cggcctcgcc catcggctca acaactaccc
    33361 gcgcaacatg tcgggcggcg agcagcagcg cgttgcagtc gcccgagcga ttgcgagtgc
    33421 gccttcgctg atcctggcgg acgagccgac ggcggcgctc gacaccgaga acggccgcgc
    33481 cgtgatggcg ctcatcaagg aaatctcgcg cgacaagagc cgcgccgtgc tcgcggtgac
    33541 tcacgacccg cgcacgctgc cgttcgcgga tcgcatcatc cacatcgagg atggccgcat
    33601 tgtctcggac gagcggcccg cgccaagcgc cggcgagagc gcagccaagc cggaggcgac
    33661 gcccaagccg gccccgcctc cggcgtcggc cgacacgccg gcaaccatcg cgccgatcat
    33721 agtcagggtg gagccgcagt cgtcgcagcc cgcgagcacc gcgccgccac agcagatcag
    33781 cattcgccag aagcgcaaga acaggaagag gcggaaaggc catggctaag aaatccgcgg
    33841 cactgcttgg cctcgtcgtg gcggtcgccg cctcggcgct cacggcattc gccgtcacgc
    33901 agctcaacga cccgcgtcgc caggatcgcg acaaggccgc tccgccgccc cgcgcgctgt
    33961 gggcggcagc ggcccctggc cgcatcgagc ccaagggcgg agagttcagg atagggccgc
    34021 agtcgtcggg gcgcatcgtc gacgtcgtgg tgcagctcaa cgaccgtgtc gttgccggcg
    34081 agctgctcgc acgcctcgac gatgaagagc tgctgacccg catggccgcc atggagaccg
    34141 agaacgcggt caggaagcgc gagcgcgacg ccgagacagt gaaccggctg gcgctcgacc
    34201 ggcgcaacgc cgaggacgcg ctctcaaatt cggagcgtgc cgtcgcgaag gccctccacg
    34261 aggtcgatcg catccggacc agcaccaagg ccggttcggc cgccgccgat gccttgatcg
    34321 cggcgcagga tcggctcgcc gccgctcgca acaagctcct cgccgatcgc gatgccttgg
    34381 cgcgtctgca ggcaacgcaa ggcatgccgc tgccgacgcg cctcgaggca aacctctctg
    34441 cgggccgcgc cgatctctcg ctcctcgaaa ccgcgctcga gcggctgcgc gtccgtgccc
    34501 ccgcggccgg gagcgtgctc cagctccacg ccaaggtcgg cgagaccgta gcaccctcca
    34561 tcgagcatcc gctgctcgtg ctcggcgacc tgacggcgct gcgcgtccgc gccgaggtcg
    34621 aggagcgcga ggcgccgaag atccgcatcg ggcagaaggt ggtcgtgcgc tcggacgcgc
    34681 atcccggtcg cgacttcgag gggcaggtcg tggtgatcgc gcagtcgctg agcgcgccca
    34741 agctcggtgc ccgcggaccg aggaagccga ccgacgtcga tgtgctcgag gtgcaggtag
    34801 agctcgccgg cggcggtccg ctgctgcccg gcatgcgtgt cgacgtgctc ttcaagccgg
    34861 atgcgacggt cgcgaccgag ccctccccga aggcatcgag caactgagcc tctcatgcta
    34921 tccctcgtct gttgcatcgt tgagcggcgg ttcgggaggc tggcatgacc gctgagagca
    34981 ggatccgcga gcagatcgtc gccaccgggg cgtcgctgtt caaccgcggc ctcacgcacg
    35041 gctcgaccgg caacatctcc gtggcgcttc cgggcggcgg atggttgatg acgccgacgg
    35101 gctcctcgct cggcgcgctc gatcccgcgc gcctctctcg cctcgatacc gtcggcagac
    35161 acatcgccgg cgatccgccg accaaagagg cgatcctcca cacactgatg tatgcgcggc
    35221 gcacggggac cggcgcggtc gtgcatcttc attcgacgca ttcggtcgct gtgagctgcc
    35281 tccagggcct cgatccagcg aacgcgctgc caccgatcac cgcctactac gtgatgcgga
    35341 tcggggcgct ggcgctcgca ccctatttcc cgcctggcga tccgaagctc gccgaggcgg
    35401 tcgctgacct cgcggacaag caccacgcga tcctgcttgc gaaccacggc cccgtcgtcg
    35461 ccggcaacag cctcgctgcg gcgacggacg cgatcgagga actcgaggag acggcgcgcc
    35521 tcttcctgat gctcaggcgc gaagtgatca cgccgctgac cgcggagcag gtcgccgagc
    35581 tgcgcaagcg ctttggctaa ccagcctccc cgccgaggct ctgcgagcgc acgagccgcg
    35641 cgtaggcgcc gtccgccgca atgaggtcgc cgtggcgtcc cgtctcgatg atgcgcccgt
    35701 cctccatcac gcagatcata tccgaggcct gcacggtcga gaggcgatgc gcgatgacga
    35761 gcgtggtgcg gcctttcgtg aaacgggcga gcgcctcctg gacaaggcgc tccgattcgg
    35821 tatcgagcgc gctcgtcgcc tcgtcgagga gcaggatcgg cgcatccttc agaatcgccc
    35881 gggcaagcgc gaggcgctgg cgctgaccgc ccgagagacg catgccgcga tcgccgatca
    35941 ccgtctcgta gcccgccggc tgctcgagga tgaactcgtg tgccgccgcg gccttggcgg
    36001 ctgcaacgat ctcggcctcg cctgcgccga gccgcccgag cgcgatattg gcgcggatgg
    36061 tgtcgtcgaa gagagtgacg tcctggctga cgagagagat cgcatcgcgc aggctcgcaa
    36121 gcgtcacgtc gcgcacgtcc tggccgtcga tcaggatgcg gccggcgctc acgtcgaaga
    36181 ggcgcggtac gagattgagg atcgtcgatt tccccgatcc ggacctaccg acgagcgcgg
    36241 ctgagctgcc ggccgggatg ctcaggtcta cccctcgcac ggccgcacgc ggcgcgcgtt
    36301 caccctcgcc ctgcgggccc ggatacgtga atgagacgtc gtcgaggcgg atcgcgccgc
    36361 cggcgaccgc gagtggcttc gcccctggcc gatcgacgat tttcggctcg tcgtcgatga
    36421 tcgcatagat gcgctcggcc gcagccaatc cctcctgcac cttcgcattg agattaccga
    36481 gtccgcgcgt cggctgcgcg gcaagcacca aggccgtcac gaacgccatg aaatcgccga
    36541 tcgagccgat ccccgaggcg atccgccagg tggcgagtgc gatcacgccc gcaaccgcga
    36601 tgccgccgag cgcttccagg atcggatcga gctgagcgcg gttgcggacg gccttcatgc
    36661 gcagcgcgta gatctgctcg aagctgccgt gcatgcgctc ggccgcatac tcctcgagac
    36721 ggaacgtctt gatgaggcgg acgcccgaga gcttctcgga aagctgcgcc gtcatgtctc
    36781 cgagctcggc ctgcgtgcgc ttcgccacgc gcttcaggcg cttgccgata gcgatgatcg
    36841 gcaggatcgc cagcggatag acggcgacca cgatcagcga gagcagcggg tcgcgccaga
    36901 tcatgtagat gacaagcccc gttaccatca gtgcatcgcg cagcgccgtg ttgaggcccg
    36961 cctgcagcgc ctgctggatg agtgccacat cattggtgag ccgcgagacg aggtcgcccg
    37021 gcgagtcgcg tgtgaggcgc gcatagtccg ccttcaggag ctggcccagt gcgcgcttct
    37081 gcatggcgac gccgatccgc atgacgatgc ggttggtcag cacattggtg aggtagagga
    37141 ggatcgagcg cgtcgttgtg atcgcgatga tcatcgccac gatttgcggc atcaggccga
    37201 gctgatcctt ggtcagcgcg tcaaatgaat acttgatgac ctgcggatag agcccggtga
    37261 cggccgccaa tgccaccatg gcgaggagcg agagcgcgag cgctccgcgc cgcggccaga
    37321 cccagtcgcc gaggacgcgc ttcaggaggc tgcgagtctt cgggtcgaga ccgagcggag
    37381 ccgtggcggc cgccttgagc tccccgtctc ccgacacgtg tgcctcctcg ccgaatgcct
    37441 cgggcgtgcc taacaggcgc tcctggccaa ggccatgcca ggaattgtca cgccgttgac
    37501 catggcttca gaagtgctca acgttaacag tgcatcgccg gtccgcgccg cggcgggggt
    37561 gtcgtgaccg cgtctcacca gcacaagagc ccggcagccg gacccacacg ccgccccagg
    37621 aggccgctca tgcatgccct gctcgcactg atcgtcgttg ccgtcgtctt cctcggctgc
    37681 gcctgaactc aaccgccctt gcgctcgagg acgagtgcac cgcccgtccc gcggcgcgcg
    37741 gcaaaaagcg ccgagacgaa cgtggacgtg ccggcctcga ggatcagcgc cggaccgtcg
    37801 accgactgtc cgtcgccgat cgcatcgcgc cgataggtcg gaatggcgag gcgccggccc
    37861 tgccgctgat cgtaggcttg gacggtgccg gtcgcctcca gcgcgccggc cgaccttgat
    37921 gccgccgcgg gcacagagcg cgggatcggt gaccccgcgg gtgtcgagac gagcacggac
    37981 cagctcagaa tctcgatctc ggcgcccgga atgtgccgct cgaagagctg acggtagccg
    38041 gcctcgaacg cctcgcggat tgcagccgca tcgccggcga cgatgccacg atttggaagc
    38101 ggcacggcga tctcgtggcc ctggcccgta taacgcatgt aggcgacgcg ctgctcctcg
    38161 agcggccgac cgagggccgc ggcctccgcg agcccgcgcg cctcgcggct catggcaccg
    38221 aggagatcgt tggccatcac gaggtcgagc tcgcccaatc gcatgaagcg gctgcgcacg
    38281 agttcgtagg cggcgggggc gcgaaggaac ccgatcgccg agccgacgcc cgcgccctcc
    38341 ggcacgacca cgcggcggat gccgagcttc tcggccatgc gcgcggcatg caggggagcg
    38401 gcccccccga aggcgatcag cgtgtgctcg gcgataatca caccacgctc gacggcgtgc
    38461 acgcgcgccg cattcgacat gttctcgtcg acgatctcgg cgatgccgtg ggccgccgct
    38521 tcgacggaga gcccaagcgg cgcgccgacg gatgcggcaa ccgctgtccc tgcattggca
    38581 ggcttcagcg tgagcttgcc accggcaaag cctgcgacgt cgatgcgccc gagcacgacg
    38641 tcggcgtctg tgaccgtcgc ctccgtgccc ccgcgaccgt agcaggcggg gcccggcacc
    38701 gacgccgcgc tgtcgggccc gaccgtgata cgacggagcg tgtcgacgcg cgcgatggag
    38761 ccgccgcccg ccccgatctc gaccatctcg atcaccggga cccgcacagg aagcccgctc
    38821 cctttcagga agcgcgcggc gcggtcgacc tcaaaggtgc gcgccttcat gggctcgtag
    38881 tcggtgatga ggcagacctt ggccgtggtg ccgcccatgt cgaaggagag caccttcttc
    38941 tcgccgagct cggccgcaat cgccgccgag agaatggcgc cgccggccgg acccgattcg
    39001 accaaccgga ccgggaagcg ctgcgccgtc tctagcgagg tcaggctgcc gccggacgtt
    39061 atgagatgga tcggccggcg atagcccgcc tcggcgagcg accgttcgag acggccaagg
    39121 tagcccgaca tcagcggctg gacataagcg ttggccacag ccgtcgaggt gcgctcccac
    39181 tcgcgcatct cggggcacac ctccgaggag agtgtcaccg agagacccgg cagcctcttc
    39241 gcgaggacgt cgcgcgtccg ctcctcgtgg atcgcgctca catacgaatg cagataggcg
    39301 accgcgaccg cctcgatgcg ctcggccgcg agcgaggcca cgatcgcctc gagccgccgc
    39361 tcatcgagcg cgaggcgcac cttgcccgtc acgtcgagcc gctcgggcac gccgaaacgc
    39421 agccgtcgcg gcaccagcgg cttcggcttt tcgatcatca ggtcgtactg atcgaagcgg
    39481 ctctcgtcgg cgatctcgat gacatcgcgg aacccttccg tcgtcagcag cgcgacgcgg
    39541 gcgcccttgc gctcgatgat ggcgttggtg gcaagcgtcg tgccgtggat cagcacgtcg
    39601 atgtcggcga aggagcggcc gagctctgca agcacatgcc gcgcgccctc gaggatcgca
    39661 cgctcgggcg catcgggcgt cgtgagcacc ttcgtggcgg cacccttgtc ggatgcttcg
    39721 agcacgacat cggtgaaggt gccgccgacg tcgacggcca gtctgagggc gcccgcgccc
    39781 attcgttctg ctgtcatgct acgcgcgcca tgccgttgtt gaggatgacc acgtcacgct
    39841 cgaggcagcg ggcacggaag gagatggtcg ccccgtcgcg ccagatctcg gtgcggatgg
    39901 tctcaccggg gtagatcggt gccgagaagc gcacttgcat gtgcgtgagg cgcgccggat
    39961 cgtcgccgca gcaggccgcg agaacggctc gaccggctat gccaaacgaa cagagcccat
    40021 gcagtatcgg cgcccggaag cccgccgccg caccgacctt gggatcggca tgcaaggggt
    40081 tgtagtcacc ggagagacga tagatgaggg ctgcctgcgg cagcgtccgc atgtcgcgct
    40141 gctcgtcggg cttgcgtgtc ggcaacgcgt ggatctcggg tgccgtgccg gtcgagttgc
    40201 cgaaaccgcc gtcccctctg aggaaactcg tcgactgcag tcgggcgagt gtcgtgcccg
    40261 atgtcttgtc gatcacctcg cgctcggaga ggaggagcgc gcccttgtcg cgccccttgt
    40321 cgatgaggcc ggtaatgcgc gttcgcccga tgatcgtcgc agcctttgga ataggcttca
    40381 gcagcgtgac cgactgctcc ccgtgcacga cctttttcca gtcgacgccc gtctcggggt
    40441 tcttcaccca gaagcccggc gagccgagca cggccgccat ggtcggcagc gccttgagac
    40501 catcttcgta gacgaaggtg agatcgcgct catcgagcgg atcggcgccg cagccgaggc
    40561 cgagcgcata gagcatcgtg tcgcgctcgg tgtacgtctg ctggacgtcg gcaatcttcc
    40621 agctgcgcag cttttcagga tcaatcggca tcagggcctc ggcggtatag atcggatgcg
    40681 gcggccaagg tgcacgccga cgactgtggc gtcaaacgca cgaagccgga tgaatgacag
    40741 ccatgcaccc gggccgcgac ggattgcgcc ctttcgcgat cctgtgaaaa ctctctttga
    40801 tgccgtaatt ccaacccctt gaccaacgta ggtcagcaac cgggcggcgg tcgcggccca
    40861 cactttgggt tcgaaatccg gcacttcgca aactggcact tcgggcacga caaggaggca
    40921 gcgtggacgg cttcatgaag atgttcggcc atgtggcgaa tatcctgaaa gggtacgcca
    40981 tcgcgatcgg tgtcgccgtg gctatcgcct tcgtgtggat gtggtggaag cagcgccagg
    41041 aggaggcgcg gactgagcgc caggtccgca tccagtccct tttcgccgag caggcccgcc
    41101 ttgccctggc acatctcgac atcgccaaac cgcggctcgt ggctcagatg tctccggagg
    41161 agaccacgcg ctaccattgg tacctcatgg aggtcgtcgc gataaacgga cagatcgccc
    41221 ggctcgataa cgagggccaa tggcgcagtg caatcgtcga ccggcttaga gtacatcggg
    41281 aagcattatc ctccaaagag ttcgaattac ttagccattc atctgtcagc agtgaactcc
    41341 gacagctgat aaaacagctt gagcagaggc attgacgtaa gtaatccaca gcacgtcttg
    41401 agcatgccga tacagtttgc aaattttgag gtttcatgct taaactgcac acagaatatt
    41461 ttcgtcggtg accgtcgaag tgggaatgtc gaatggttgc tcgccgttgc tcatcgacaa
    41521 ccggattagc cgggcaggac tcagtcgact agatcgcgaa ctgaaccggc cggcagccgg
    41581 tcgtgagacc agtcagatcg aatcaaagaa caaagcgtga cttgctagta gcaaggtctt
    41641 tcccagtttg gcgcgtagag tgaggagtca atgttcacaa gnnnnnnnnn nnnnnnnnnn
    41701 nggatcgcgg gcctcgccat gttggtcgca ggcggaaccg caaccgtcta tgctgcgggc
    41761 ggaccgggca cgcagatcaa cgacgggcaa tcactgattg ccgccttcaa gtgtctcgcc
    41821 gatggcaatg ccacatgcga gtcctttgcc cgcgcagctg cggcggcggc caagcacaat
    41881 tggcagatcg ccgctctgat cgccggagtc ggcgcgctct ggatcggcac ggccatccgc
    41941 atcgcaacgg aacttcgtgc catgagggtg ccgccgacca ggcagcgctg acacgcgtac
    42001 cgggacaatg cttatccgct cgccgtcggc ctcatccgtg gtcggcggcg aacgtctgtc
    42061 tgcgcgccgt gccgatcgag acaagatcgc gcccgcactt gcgacgctta tctcgattgc
    42121 caggggcgcc ctcatgcgat agcgaacgga cgtatgcacg ctggggcgcg cgtcggctcg
    42181 aaactggggc tctcgaccgg ctcgatcctg tcgtgtcgga agagacacgc gtccaagaga
    42241 ctttcgtcaa aattgaacgt gtcggacgag acaggggaag cgggatacga tcgggcatgc
    42301 cgaaacagct gatcatcgac gggaagccga tcgaggtgga ggatcacctc acgctgctcc
    42361 aagcctgcga acaggccggt gccgacatcc cgcgcttctg ctatcacgag cgcctctcga
    42421 tcgccggcaa ctgccgcatg tgtctcgtcg aggtggtcgg cataccgaag ccacaggcat
    42481 cgtgcgccat gggcgtgaac gacatgccgc cgcgccgcga tggcaagccg cacgagatca
    42541 acacgcgctc cgccatggtc aagaaggcac gcgaaggcgt gatggaattc ctgctgatca
    42601 atcacccgct cgactgcccg atctgcgatc aggccggcga gtgcgatctg caggaccagg
    42661 cgatggcgtt cggcaagggt ggctcgcgtc acgtcgagcc gaagcgcgcc gtcgaggaca
    42721 agcacctagg cccactcatc aagacgatga tgacgcgctg catccactgt acgcgctgcg
    42781 tgcgcttcat gaccgaggtc gccggcgtcg aggaactcgg cgccatcggc cgcggcgagg
    42841 acatggagat caccacctac ctcgagcgcg gcatcatgtc agagctctcg gcgaacgtcg
    42901 tcgatctctg tcctgtcggc gcgctgacac atcgaccctg ggccttcgct gcgcgccctt
    42961 gggagatgcg caagaccgaa tcgatcgacg tcatggacgc ggtcggctcg gcaatccgga
    43021 ttgatgcgcg cggtgctgag gtgatgcgca tcctgccacg caacaacgac gctgtgaatg
    43081 aggagtggat ctccgacaag gcgcggcatg tagctgacgg tctgcgcacc cagcgcctgg
    43141 atcagcctta catccggaag aacggtaagc tcgttcccgc aacctgggac gaggcactcg
    43201 ccgcggtcgc cgagcgactg acagccgcga cacccgatcg catcggcgtc atcgccggcg
    43261 atctcgcggg cgccgaggag atgttcgccc tcaaggatct cgtgacgcgc ctcggcgtga
    43321 cttcgatcga ctgccgccag gatcgctccg agctcgatcc ggccctcggc cgggcgagct
    43381 acaccttcgg cgcaggcatc gaggccatcg agcaggccga cgccattctg ctcgttggca
    43441 ccaatccgcg cctcgaagcg cctgtcctca accagcgcat ccgcaagcgc tggcgccagg
    43501 gcgccctcaa ggtcggcgtg atcggccagc agtccgacct cacctatcag acaacctatc
    43561 ttggtgccgg gccgcaaacg ctcggcgagc ttgtcgatgg caagcacgcc ttccgtgagg
    43621 tcctcgacaa agcggccgcc ccactcgtca tcgtcggtgc gggtgcggtc agccgcagcg
    43681 acggtcgcgc cgtgctctct gccgccgcgc ggcttgcgac ctccgtctcg gctgacaagc
    43741 ccgcgacatg gcgcacgttc aacgtattgc acaccgcggc ttcgcgcgtc gccgggctcg
    43801 acctcggctt cgtgccgact gggagcggtc tctccacggc gcacatgctg acggcggccg
    43861 agcgcggtgc gctcgacgtc gtctatctgc tcggcgccga cgagctcgac atggcgcgcc
    43921 tcggcaaggc cttcgtgatc taccatggca ctcacggcga tgccggtgca catcgcgccg
    43981 atgtcattct gccggccgcg gcctacacgg aaaagagcgc aacctacgtg aacaccgaag
    44041 gccgcccgca gcagaccgcg aaggccgcct tcgcgcccgg tgacgcgcgc gaggattgga
    44101 cgatcatccg cgcgctctct gcccgtatcg gcaagatgtt gccctacgac accctcgagg
    44161 cgctccgggc caacatgtac ttgaacgtgc ccgctctcgc acgtatcggc gcaatcgagg
    44221 cagggacagc tgacctctct cgcgcagccg ttgtcggcgg cccgctctcg agcgagccct
    44281 ttgcacccgt catccgcgac tattatctca ccaacccgat cgcgcgcgcg tccgccgtca
    44341 tggccgacct ttccgcgctc aaggccagca tgaccgagga ggcgaccggc accaatggct
    44401 aatatcgtct gggccgacat ctggcgcgat tacggctggc ctttgctgct catggtggtg
    44461 cagagcctgg tgatgctcgc ctccgtgctc gtcattctcg cgttcctgct cctgttcgac
    44521 cgcaaggtgt gggccgccgt ccagcttcgt cgcgggccga acgttgttgg ccccttcggc
    44581 ctcctgcagt cgttcgccga cctgttgaag ttcgtcttca aggagccgct cattcctgcc
    44641 ggcgccgata agggtgtgtt cctgctcgcc ccgctcgtca ccgccgtctg cgcactcgcg
    44701 gcatgggccg tgatccccgt ttccgaaggc aacttctttg gtctcgcgtc gggaaagtgg
    44761 gtgatcgccg atttgaacgt cggcatcctc tacctcttcg ccatctcgtc cctcggcgtc
    44821 tacggcatca tcatgggcgg gtgggcatcg aactcgaagt acccgttcct cggctcgctc
    44881 cgctccgcgg cgcagatggt ctcctacgag gtctcgatcg gcttcgtgat catcaccgtc
    44941 ctgctggtcg cgggctctct caatatgagc gagatcgtca acgcgcagcg ctcgggtctc
    45001 ggaacgaaga tgggcctccc ctcgtcagcc ctcgactggt actggctcgc gctcctgccg
    45061 atgtttgtcg tgttcttcat ctcggcactt gccgagacca accggccacc cttcgacctc
    45121 cccgaagccg agagcgagct cgtcgccggc ttcatggtcg agtactcctc gacgccttat
    45181 cttcttttca tgctcggcga gtacgtggcg atctgcacga tgtgcgcgat gacgacgatc
    45241 ctgttcctcg gcggctggct gccgcccgtc aaccactggc ttttcaactg ggttccgggc
    45301 gtgttctggt tcctggcgaa ggtcgtgttc gtcttcttca tgttcgccat ggtgaaggcg
    45361 atggtgccgc gctatcgcta tgaccaattg atgcggctcg gctggaaggt gttcctgccg
    45421 ctctccctgg tcatggtcgt gatcgtggcg ggcgcgttgc agtacggccc gcagctcatc
    45481 gggaagtgag tcatggacct caacaagatc ttcggcacgt gcctgatcgt cttggggctc
    45541 atcgatgtct tcatcctgcc gcgcttcatg cgccacggcc cgccgatcat aacctcgatg
    45601 gtgcgcttga tcggtctcgg cttcatggcg gcgggcgtgg tgctggtcct cggattcgtc
    45661 aagtttctct cttgacgccc atcccgagat cgccgtcatg ccgacgacac gagaaggact
    45721 ctgaaagtga acattgcagc cggcatcaag tcactgttcc tcaccgagtt cgtctcggcg
    45781 ttcttcctca ccatgcgcta cttcttcaag ccgaaggcga cgctcaacta ccccttcgag
    45841 aaagggccgc tgagcccccg cttccgtggc gagcatgtgc tccgccgcta tcccaacggc
    45901 gaggaacgct gcatcgcctg caagctctgt gaggcgatct gcccggcaca agcgatcacc
    45961 atcgaggcgg gtccgcgccg caacgacggc acgcgccgca ccacgcgcta cgacatcgac
    46021 atgacgaagt gcatctactg cggtctttgc caggaggcgt gcccggtcga ggccatcgtg
    46081 gagggtccga acttcgagtt cgcgaccgag acgcgcgagg agcttttcta cagcaaggag
    46141 cggctgctgg cgaacggcga ccgctgggag cgcgagatcg ccaagaacct cgccatcgac
    46201 gccccctatc gttgacttgg cacttggtgc aaaatgggct tcatgtcgat cgataccttg
    46261 accatcgcca aacgcttgac cgacgcggtt ttttccgtgg cgcaagcgga agcgcgggcg
    46321 tcggtctgga aggagatcgt cgaaagcgat ctggccacga agcgcgactt catggaggtc
    46381 gaagccggcc tcaagcgcga catcaaggag ctcgacacct cgctccgcgg tgagatcgtg
    46441 caatcagtgc tccgcacgcg cgcgcagatt tccgagttgc aggcgaccac tctccgcgtc
    46501 gccatggcga tgacagcctt tacgatcacg gccatcggat tgatgatcca cttccttggc
    46561 cgttgaacag gaatcaaccg gaagatcgag cccagacgac catgacgctt gcccccttct
    46621 tcttctacct cttcgcagcc acgaccgtgg tcgcaggtct catggtcatc acggcgcgca
    46681 accccgtgca cgcggtcttg tggctgatcc tcgctttctt caacgcggcc ggcctcttcg
    46741 tgctgctcgg cgccgagttc ctcgcgatga tcctggtcgt cgtctacgtc ggcgcggtcg
    46801 cggtgctctt ccttttcgtc gtcatgatgc tcgacgtcga ttttgcggag ctgcgcgcgg
    46861 gcttcatgaa gaatgccccg ctcgggctgc tgatcggcgc cgccgtactc ctcgagctca
    46921 tactcgtcgt cggcgcctgg gcgctcaagc cgaccgcggg cctcgtcgtt cgcgcgcctg
    46981 ctcctgacat cgccaaaatt tcgaatacgc gcgcggtcgg ccagctcctc tacaccgagc
    47041 cctacgtcta cctgtttcag gccgcaggcc tcgtgctgct cgtcgccatg atcggagcca
    47101 tcgtgctgac gctccgccac aaggaaggcg tccgccgtca gagcatcgcg gcccagaacg
    47161 cgcgcacgaa ggctgccgca atgacaatcg tcaaggtcga gccggggcag gcacagattc
    47221 cctgacaccg cggtaccgga cggcgaacgc ggctcgcgtt cggacgcgaa tggagacgta
    47281 aagtcatggc tgaagcactc gcctgccagc gcgccctctt cgacattccg gacgatgtcg
    47341 cctacatcaa ttgcgcctac ctttcaccgc tcatgcgtgc cgtcgcggac gcgggacacc
    47401 agggcgtcga tcgcaaggtc catccctgga cgatcacgcg gcgcgagttc tatgcagagc
    47461 tcgatgccgt gcgcggcggc ttcgcgcgcc tcatcggtgc caccgccgac gatatcgcca
    47521 tcgtaccatc gtcgagctac gcggccgcga ccgccgccgc caacctcccg ctcgccagcg
    47581 ggcagaccgt cgtcgtcatc gccggcgagc acggttcgaa cgtgcgcatc tggcatcacc
    47641 tggcgcgcga tgcgggcgcc agcatcacga ccgttgcacg acccgccgat gacgattgga
    47701 cgagcgccgt cctcgccgcg atctcaccgg cgaccggcat cgtggcactc ccgccgctcc
    47761 attggacgga cggcagtacc ctcgacctcg cccggatcgg tacccgcgct cgcgagctcg
    47821 gcgctgcctt cgtcgtcgat gcgacacagg ccgtcggtgc cgtgccgatc gatgtcgccg
    47881 agttgaagcc tgatttcctg atgtgctcgg cctacaagtg gctgctctcg ccctattcga
    47941 tcggcttcct ctatgccgcg ccgcatcgcc aggcgggccg cccgctcgag cagcacagct
    48001 tcaatcgcgc cggcgccgaa caatccgagg gttcgaccga ctatccggaa gctttcgagc
    48061 cgggcgcgcg ccgttacgac gtcggcgaac gctcgaactt tattgcgctc cccatgctca
    48121 aggtcgcgct cgaccagctc aacgcctggg gaccggcgcg catcgcggcg tccactcggc
    48181 cgattacgga gcgcatcgtc agcgaagcgg cgaagctcgg cctgagggcc ccggccgcgg
    48241 cattccgatc gccgcacatc gtcgggctga cgcgaccggg aaagactttc cccgccgacc
    48301 tcgggcagcg gcttgcggct cgcaaggtct acgtttccat acgcggcccc gcgatccgca
    48361 ttagcccgca cgtctacaac aatgctgccg acgtcgagcg actcattgcg ctgctggcgc
    48421 gtgaggccgg ctgagctctg cgcgaacggg gctgccgctc cttaccccgc ccggaggaga
    48481 tcctccggac ctccctttct tggactgaaa gtaagtgcgc tgcgtcctcg ctacgtcttg
    48541 aggagtcccc gctccttcag caccttctcg gcaaccttga aagcctcgat ggtcgctggg
    48601 atgccgcagt aggcgaccgt ctggtagaga acctcctgga tctcctcgag cgtacagcca
    48661 ttgttgatcg cggagttcac atgcagctcg agctcggcgg gctttcccat cgccgtcagc
    48721 atcgccatgc acagcatgct gcgcaccttc ctcggcaggc ccggccgcgc ccagatgtca
    48781 ccgaacgcat acgtctccga gaactggcgg agcggacggt tgaaatccgt ggtcgaggcg
    48841 tcgcgcttcg ccatgtacgc cgagcccagc acctcagtca gtatggcgcg ccccttattg
    48901 cgttgctcct cgaccgacat cggcatctcc acggtttggc tccgagcgcg tcggccggcg
    48961 ctcctcgctc aggaggcggc ccgcgcccgg tgaagatgcc acaccgccaa ggcgaacacg
    49021 accgccgcaa agccctggtg gacgaggccg agcgacagcg gcacacccgc gagcagcgtc
    49081 caaatgccga atgctgcctg cgccgtcagt gcgagcgcga gaaaaagagc ggagagagcg
    49141 atccggctcg tgccgtcgcg ccgcatctcg agtactgcat gcccccagct cgccaccagc
    49201 agagtgtagg cgagcaggcg gtgatggaac tgcaccgtca gcatgttctc gccgaggttc
    49261 acccaccacg gcgactttgc aaagagcccg gcggggacga ggcggccctc catcagcggc
    49321 cacgtgttgt agagaagtcc agccttcatc ccggcaacga atgcgccggc aatgacctgc
    49381 acgagcagga gtgccgtgag cgacgccgca aaaatccatg cggcgcgtgc gccgccgccg
    49441 tcgcggctcc cctgacgccc gtcccgtgca agatcgagcg caacccagac gagcgcgccg
    49501 agaatgacga acgcgatcga aaggtggacc gcgaggcggt attggctgac cgaaaccagc
    49561 tcgacgagcc ccgaggccac catgtaccag ccggccaccg cctgcagccc gcctagcgcc
    49621 aggatgccga ggagcttgag gccgagcaca cggtcgatcc gacccgtcca ccagaaccag
    49681 gcgagcggca gcgcgaacac gatccctatc gcgcggccga gcgcacggtg cccccactcc
    49741 caccagaaga tcgctttgaa ggcgtcaagg ctcatgccct tgttgacgag cttgtactgc
    49801 gggatctcct tgtacaaggc gaacgccgcc tgccaggctt cgtccgagag cggcggaata
    49861 atccccatga tcggccgcca ttccgtgatc gagagccccg accccgtgag ccgcgtcgcc
    49921 ccgccgacga tgaccatggc gaagacgagc gctgccgtga gccagagcca ggcttggatg
    49981 atccctcgag ctccgtgatg cgtcgcgcgc gccgccgcaa tacccgctgc catgtcgtcc
    50041 gctcgcaagt cgcttcgatc ctggtcttac gctcctgcta taaagcatga tcagcgacaa
    50101 gggccgggct gcggcaccgt gccctctcgc ccccacccca ccgtcatccc gccagagggc
    50161 gcgacccggt gcactttggg ccagtccgca ccagtcccac gcaagatgac ctggggcgtg
    50221 gccttcgcca ggatgacgga cgtgagtcac aatcgcgagc gcgaacccac gccatcacga
    50281 acccgcacga tctcaaaccg ccttggcaag ctcgcgcagc ttgaacttct ggatcttgcc
    50341 ggtcgaggtc ttcgggatct ccgtgaagac gacgtgacgc gggcatttgt agcgggcgag
    50401 atgctcgcgg cagtagtcga tgatctcggc cggctcggcc ttgatcccgg gcttcagctc
    50461 gacgaaggcg cacggcgtct cgccccactt ctcgtcgggc ttggcaacga ccgcggctgc
    50521 cgacaccgcc ggatgcttgt agagcacgtc ctcgacctcg atcgaggaga tgttctcgcc
    50581 gcccgagatg atgatgtcct tcgagcggtc cttcagctgg atgtagccgt cggcatgcag
    50641 gacgccgagg tcgccggtgt ggaaccagcc gccggcgaag gcctcctccg tcgccgcctt
    50701 gttttttagg tagccgcgca tgatgatgtt gccacgcatc atcacctcgc cgatggtttg
    50761 cccatcggcc ggcacgcgct gcattgtctc ggggtccatg acggtcaggt cctcgagcgc
    50821 ctggtagcgc acgccctggc gcgccttcat cgtggcgcgc gcgtccgcgg cgagcttgtc
    50881 ccagtcgctc ttccagtcat tgatgacggc ggggccgtag ctctcggtca gtccgtagag
    50941 gtgcgtgacg ttaaatccgg catctcccat tgccgcaagc acggcctcgg gcggcggggc
    51001 ggccgccgtc atgaactcca cgcgatgcgg cagtggccgg cgctcgtcgg ggggcgcgtt
    51061 gagcagcgtc gacatgacga tcggcgcgcc gcacaagtgc gtcaccttgt ggtcggcgaa
    51121 ggcgtcgaac atcgcctttg cacgcaccca gcgcaggcac acgtgcgtcc ccgcgaccgt
    51181 cgagatcgac caggggaagc accagccgtt gcagtggaac atcggcagcg tccaaagatn
    51241 nnnnnnnnng acgggatgct tgccgagccc ggccgcaacg acgttggcat agcccatctg
    51301 gtgagcaccg cgatgcgagt agaccacgcc cttcgggttg ccggtcgtcc ccgaggtgta
    51361 gttgagcgag atcgcctccc actcgtccgc cggccagcgc caggcaaagt ccgggtcgcc
    51421 gcggccgatg aactcctcgt agccgaacac gccgagccgg tcgccggtct gggggaactc
    51481 cgggtcgtcg tagtcgatga tcagcggcgt gacgcggcaa tgcatcagcg cttccttgat
    51541 cgtcttcgag aactcgcggt cggtgatgag cacgcgcgag ttggcgtggt cgagctggaa
    51601 gccgatgatg gccgcatcaa gccgcgtgtt gagcgagtgc aacaccgcgc cggtcatcgg
    51661 cacgccgtag tgcgcctcga gcatcggcgg cgtattcgag agcatcaccg agaccgtatc
    51721 gccagggccg atgccgcgct gcgcgagcgc ggaggcgagc cgacgcgagc gcgcgtagag
    51781 ctcgcgatag gcgaagcgct gctcgccgtg gatgactgcg gtgtgctccg ggaacacgag
    51841 cgcggcccgc gccaggaacg agatcggcgt cagcggctgg tagttcgcgg gcgtcttccc
    51901 gagatcgact tcgtacgggt tcatgctcat gggggcgacg gtccttcgca cacgcccgac
    51961 aagccgggcg gaatcgcgcc aggacattag cgaccagctt gccgcatcgc aatgcagcaa
    52021 agggtggcgt tccgccctca ctcacgttgc ctggtccggt ggccgtatcg tcacgtcgac
    52081 accaccggcc gtcccccaaa ggggccaagg ctccgccaag gccttgctgg ctcgccacca
    52141 cgcaccggcg aaacgggttc gtcatcctgc tggctgctcg ttcacctcga gctgctctcc
    52201 accccgcatc tctgcggcgc agttgcctcc ggctacgtgt gtcatgacct cacatggcaa
    52261 ggactttcac ctcctgacaa agcagcatcg cagccgtaca cggccccgga cgagcgctca
    52321 cgcgctctcc ggggaaccag cgggcggcat tggggagccg ctcgccttcg gcgattgcga
    52381 agtaccacat gagcggcgcg ttcccgctaa cgcgcaggca cgcctctcaa cggctgcact
    52441 tccggattac cataggcggg cttcggcagc ggtgctttca ggcgcaaccg cgtcggcatg
    52501 gccgctacgc tccgccccgg cagcgactgg aggtagcgca ccacgacctc cacgccgtcg
    52561 agcggattgc cggcgtcgtc cttcaagatt gcgatttccg tcgacggcac gacgttgatg
    52621 agcgtcggat cggccgcgta gtagtagctt gcataggtgt agctcgctgc cgggtcgagc
    52681 ggcatcggcc cgctcccgtc cgcccgcgag accatgattg cactcgcgcg cttgcccttg
    52741 ccggcgcccg gatcgatgtc cgccgtgacg cctgagaaat tgaacagcca cccgcccgtc
    52801 cagctcgcga cgtctgtcga cagcgcgcct tcggccgagt tctcgagctg gttcttgagc
    52861 tgctggccct tgatggtgcc cttggcgatc agcggtccga tcgggatgta gtggtagagg
    52921 tcctccatcc ggatcgggcc cggggcgaca tgcgtgccgt atcggaagcc gcggatggcg
    52981 ccgatgtcgg ccttggcgac ggtacggaag gcgtcggtca ggaagttgtg cgacgaccct
    53041 tcgatgacgg cgggtgcagc atcatccgtg aagcccgagc ggtgcaacgc gaccttggtt
    53101 tcgccgacga ccgtgtcgat gggccgagcg agctcggtgc cgttgaaggg gttcttgtgc
    53161 ttcgtgaagg cggggcctgt tacgaaactc ttcctcacct cggcgacctt ggcggcgacg
    53221 acacggctct cggcgatgct ctcgtcgatc agatgcggcg tccacgacca cgatctgagc
    53281 ttcccgccat cgacctcgag cgtcagctcg ccgagcatgg tgccgtcctg accctcctcg
    53341 acgatgatcg tgcccgtgct cgtctcgatc ggccggcgcg tcacctcgtg catatcggag
    53401 gagaggatca cgttgatacc ggggttcttc tcggcaaggc ggatgttgtt tgcgagctcc
    53461 tgctcggaga gcagcacgac aagatgcacg ttctcctgct cgcgcagctc tttcagaagg
    53521 cgcggcacct cctcctcgcc cgaggtgaac ttgaagccct tggtcacgtt cttgccgacg
    53581 acctgcgggc cgcgccgcgt agtgagcccc atgatgccga tcttcagcgc gccgatctgg
    53641 cggatgcgat agggcggcac gacggtcttg ccggtgcggt cggcataagg ctcgccgtcg
    53701 aagtagacat tggccgcgac cgtgccccag ggcgctttgg gcgtcccctc gccgaagaat
    53761 tcgatgaacc gcgcgggccc gtagacgaag tcccagttgc cgggcgcgaa gacatcgatc
    53821 ttcaggagat tgagcacgtc gaccatcgcc tggccgcgcg tgaacagcgc ttccgccgag
    53881 ccctggatgg tgtcgcccgt gttgacgagc agcgtattgc gccgccgctt gcggatctcc
    53941 tcgatgcgtg tgtgcatgcg ggcaacgccg cccgcggtct tggccgaagc gtcacgacgc
    54001 agattgggcc gcggcacgag atggccatgg atgtcgccga tgtggatcag cgtgactgag
    54061 cccgacggcc ccgccgccgg cttggtcgct ggggccttgg ttgcgggtgt cttggttgca
    54121 ggtgtcttgg ttgcaggtgt ctggccgtgg ccagactgcc acgtcacggc gaccatcacc
    54181 gttgccaggc ttgccacgag ccctgcgatg acctgaagtc gcatcattcg catgtcgccc
    54241 ccctgttgca cttgccgcct taaccttgcc cgatgcgttc cacagccatc acatcgacgt
    54301 tacatccggc gtgtagagta tcgagagaga tgtatactgt atttttattt tgttttcatg
    54361 ctgttacata gattgaacta atatttcacc tgaagtcaac gtcatcccgg ccatggccgt
    54421 ttctcgaaca tcttcccctc gccgccgtca caggctcccg cttgggtgaa ccgctcgacg
    54481 cctcgggttc gggtcatcac acgacaagtg gccggctgca gcggtgctgc cacggcgacc
    54541 attggccctc tcagcgcgag acccactgct cgctatcatg ctcacatgag tgatcacatg
    54601 acacctggtc ccgaggcact gacccggccg gcgagcgcgc ttgctgcacg ctacgacgcc
    54661 atcgtcgttg gctccggata cggcggcggt gtcgcggcct cgcgcgtgcg ggcaagcgcg
    54721 ttgccgtagt cgagcgcggt cgcgagttcc gcccgggcga cttcccctcg cgctttccgg
    54781 aactgcgcgc cgagatgcgc atcctcggac ggcgcatgaa cgtcggtcgc gccagcgccc
    54841 tcttcgatgt ccgcctgggc gaggacatgc atgttgtcgt cggctgtggc gtcggcggcg
    54901 gctcgctcat caatgcgggc gtggcgctgc ggcccgatcc gcgcatcttc gccgaccccg
    54961 tctggccggg
//
LOCUS       DAORNH010000002        54537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_452_length_54537_cov_10.588249, whole genome shotgun sequence.
ACCESSION   DAORNH010000002 DAORNH010000000
VERSION     DAORNH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 54537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54537
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_54537_cov_10.588249"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..689)
                     /locus_tag="PK264_00265"
     CDS             complement(225..689)
                     /locus_tag="PK264_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74362.1"
                     /translation="MKMCGQTFLSALLWASSLVCAFADVPEQKAPEEQAGMNARFCLS
                     AEALEAAIPNGLSGDIMASAAVANHYYLCMFNTEMAKKWILISAENGEAGAMSAIGVI
                     YCEELDTELNRRGLFWLKKASENGVEIASFMLSQYLKLPEKPCGRLLLEQMP"
     gene            complement(700..1833)
                     /locus_tag="PK264_00270"
     CDS             complement(700..1833)
                     /locus_tag="PK264_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRD74363.1"
                     /translation="MKRSLSKVRSRPFLVVERATGAKTFLHADRLGSIIATASNDGAI
                     TGQAKYSPYGVTTGSIPTIFGYTGHQYDREIGIYYARARTYMPLIGRFMQVDPVGYKQ
                     SLNLYTYGMGNPMAGTDPSGGFFQFIVPLLQLAAGELSASAFAGLLVNYAINYAIDTA
                     ITMALEDAYPGAGRVFGIIRSAIGLASSAGFGGGSGKVEQAATGTLDRGLLAGPAANI
                     SSTSVTSGEIYAPVGSNYKRGADVIMDIGGTGLTNGASKALFPQDEFVIAGHGVISGG
                     IVDSRANFGTQTPISPESLLPYLEVNYHYSAALANLKPGQTIRLLSCYGGGTTPTGLE
                     YAQALADASGHPVVAATSLVHLNYENGNFWLDPGGEWRIYYPR"
     gene            complement(1930..2451)
                     /locus_tag="PK264_00275"
     CDS             complement(1930..2451)
                     /locus_tag="PK264_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74364.1"
                     /translation="MIGTQRRVRSLAFVVLATVLAAGCRQECPAITGLEGSSLVLKTG
                     NYTSGDKMEVVLEIKNAPFDLDEAYLLENLDVSNRDMKSYFGWEVYNRVLSADRMTFP
                     DYSLTRVEGKLTITLIGEVETREGLPYVSFGKFGGAWWNPADGKLRLSVILYANACVD
                     DEAMLVFPSLTIE"
     gene            complement(2448..3761)
                     /locus_tag="PK264_00280"
     CDS             complement(2448..3761)
                     /locus_tag="PK264_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRD74365.1"
                     /translation="MTSAVVWAAPLPKIGLGMNGSATMGYDPAGRRDRLTISGPAGTR
                     TSFFFADGENTVAEFSGLWDVSALLRRFIYAPGASTPFLVVERATGAKTFLHADRLGS
                     IIATASNDGAITGQAKYSPYGVTAGSIPTIFGYTGHQYDREIGIYYARARTYMPMLGR
                     FMQVDPVGYKQSLNLYTYGLGNPVAHTDPSGAFIGLIIPFAQFLLGEISASAFVSTLI
                     ETVLTEGLLRGLDYLSPGAGTAVGTALSIYGLGQSVFAGSGPGKVVEFAGGAVKAGAA
                     AIVPYLTEGSRLSSYVEPQNLASVIDKAADESSYTEDISLMAGLRVPRDSRYTLTSEG
                     WFDLQHAVSAYEAFGAGPSSELMMGAIELDQLQQQLDSAFMAEDIFSNRVGAWADLTY
                     KWDVFTGPGAKIVDYLDLTGIEVYPRWKAAILCGNGSVICSGGYR"
     gene            complement(4148..5341)
                     /locus_tag="PK264_00285"
     CDS             complement(4148..5341)
                     /locus_tag="PK264_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRD74366.1"
                     /translation="MAEFSGLWDASALLRRFIYAPGASTPFLVVERATGAKTFLHADR
                     LGSIIATANANGAITGQAKYSPYGVTTGSIPTIFGYTGHQYDREIGIYYARARTYMPL
                     IGRFMQVDPVGYKQSLNLYTYGMGNPMAGTDPSGGFFIGLGIAAIQFAIGELTATAFL
                     STIAEIAIGELVTRGLDMLIPGAGQVLGIVRTVVGFITSPGFGGGAGAIKNAAHGGPV
                     NGGGLSGHFGVGDGVGLGYGFQTPKHDPLDPDDWYLENIVQRDHAEMRRCFISCTIDD
                     ALDSLAENASVSGTVGYYGALTLQVNSDGTTDYGLGIGAGASAKPDFAGSITLFEAQR
                     RDPSGWGLAATVTGQIHPILPVSFTGTFYYNPDTSAYYISLSVDGPTAGYSTFLGVYK
                     KNVFK"
     gene            5640..6899
                     /locus_tag="PK264_00290"
     CDS             5640..6899
                     /locus_tag="PK264_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HRD74367.1"
                     /translation="MARALNKLSARAVATLRTPGRHGDGGGLYLAISADGTRRRWVFL
                     YTLRGQRREMGLGAVADVSLAEAREKAMEARRIVKAGTDPIAAREATKAATRATEAAE
                     RAIVTFGAFADAYVDRMSPAWRNETHREQWRTTLRVHAAVLRDMPIDGVDTDAVLRVL
                     EPIWNRIPETAARLRGRIENVLDAAKVRGLRTGENPARWRGHLRNILPKRQRLTRGHH
                     AAMPFAQVPSFLSALRARQSTAARALEFCILTAARTGEVLGAQWDEFDLDAAIWTVPA
                     TRMKAQREHRVPLCARAVVVIREMALARMGAHVFPGHRASGLSNMAMAKALEASGQGA
                     FTVHGFRSSFRDWAAEATSFPSDICEMALAHTIGNKVEAAYRRGDLFNRRRELMEAWG
                     RYCADAERGVIRLVDLPKDALRNKIAS"
     gene            7082..7990
                     /locus_tag="PK264_00295"
     CDS             7082..7990
                     /locus_tag="PK264_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74368.1"
                     /translation="MGSTNGRETYRRAKKRQKSLSPDHIIGTVGAYWEGETHNFTKED
                     FAELHRLAKRAGSKATLEEVHDRATQVAGEVHEVLHNLTGVMPASERAKSASLIHSAI
                     GQIERALGYYQWDEDALWRTNGAGGSIAMLERLASHAFVQTNKLDREDAQNLRKDETA
                     ISRRLRALQIGVPYKGELPPPRHWRGIAIDSAINTLPVRLRVLRLACESFLQAPPAKR
                     PRVKTLRLGDRFIRSASDAWRELTGKRTRYINLEFAMVLARGIGMSVSKDALRGLIYR
                     EAKRLAGPVGNKIPIPAITAAANRQV"
     gene            8115..8903
                     /locus_tag="PK264_00300"
     CDS             8115..8903
                     /locus_tag="PK264_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74369.1"
                     /translation="MFNEADFAEFHAIAQGGGRDMSLRRVRQIVTRAAINFYDDRILG
                     ADKMTPGEKVRHVEAIRRAVRELEGRIGIEVIPEGEKVASGSRSQILGELTIEAQKLE
                     RSLDLGWLARIDKSTLQIARTLRSIAERLCRKSPLDVPGHNPRSGKLYDIMALLPTYL
                     CVLSLACDAWLLNPPEKQPKRALGSVNRTFLHAANGAYQQITGLSLSRSRQSIAFARR
                     MAGGMGFDITDPALRSLLSRNQAGTTKGYQVRRRAQTAKKTVPV"
     gene            8980..9186
                     /locus_tag="PK264_00305"
     CDS             8980..9186
                     /locus_tag="PK264_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD74370.1"
                     /translation="MTNSDISAAKDREAYSIAEAAKLWGIGRTSLYRAMSEGRLRTLK
                     IGARRLVRRGDLEAFMEAHATPTP"
     gene            9310..9825
                     /locus_tag="PK264_00310"
     CDS             9310..9825
                     /locus_tag="PK264_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74371.1"
                     /translation="MTARDRKIHGPPKGSPWVWLPTELLNHLAWRSLSLNARKIIDFL
                     LIEHRHHGGRENGNLAAPYNQLERFGVSRRLIRASFLELEAAGLVRMTRQGGKYAGGR
                     ETSTYRLTFYAAHDNVSPTSEWRRSDGAKVKAARKDAAENKSRASENRRGRRANPDLE
                     CTMVNLESAPS"
     gene            complement(10027..13257)
                     /locus_tag="PK264_00315"
     CDS             complement(10027..13257)
                     /locus_tag="PK264_00315"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HRD74372.1"
                     /translation="MAYLSEAAIEKVVLDHLGGLGYALATDAEIGPDGKAPEREAYAD
                     VVLLKRLTAAIERLNPAIPAEARSDALRKVLATEMPSLVEENRRLHKLMVEGVDVEFY
                     SDDGTIRGDKVRLIDFDDLAVNDWQATGQFTVIEGSVNRRPDIVVFINGLPLGVIELK
                     APGGETATLAGAHNQLQTYKAQIPSLFRTNAVLVISDGMTARVGSLTADRERFMPWRT
                     TDGKVIAAKGQPELDVLIEGIFDRRRLLDLLRDFTVFGETGTGLAKIIAGYHQFHAVR
                     RAVESTVRALAMNAGTSASAHRMAGFHEEPAAYGLPGVTDYPRGDKRIGVIWHTQGSG
                     KSLLMAFYAGQLVRHPAMENPTIIVITDRNDLDDQLFGTFSMCRDLIRQTPIQADSRE
                     DLQSTLSRASGGVIFTTIQKFSPAAGEATYPMLSDRRNIVVIADEAHRSQYGFKARIE
                     QKTGEIAYGFAKYLRDALPNASFIGFTGTPIEQDDVNTPAVFGEYIDVYDISRAVEDG
                     ATVPIYYESRLARIELPDEEKPKIDAEIAELTEDEAVTEQEKIKRKWATIEALVGSRK
                     RLAMVAADLVRHFEDRVAAMDGKAMVVCMSRRICVALYDLIIALRPDWHNDDDAAGEV
                     KVVMTGSAADPPSWQPHIGTKARRDLLAKRAKDPNDALKLVIVRDMWLTGFDAPSMHT
                     MYVDKPMKGHGLMQAIARVNRVFRDKPAGLVVDYIGIAQNLKNALGQYSGSDQRQAGI
                     DKAEAVAVLLEKYEVVSAMYHGFDYARGLAGTPHQRLVVLAEAIEWILGRQHDAAAKE
                     TSEDGKRRENRRYLDAVLALSKAFALASASDEARDIRDEVGFFQTVRAALVKSAEGSG
                     KSSADREFAIQQILDRAVVSTEILDILAAAGITTPDISILSDEFLVEVQQLEKKNLAL
                     EALRKLLNDEIRSRSRTNVVETKRFSERLEAAIARYHTNAISTVEVLQELIDLAKEVR
                     AARKRGEDEGLSQDEIAFYDALAESESAVELMGNDSLKVIAHELLVSLKGSVSVDWSH
                     RESARARMRVLVKRILRKHGYPPDLQDAAVQTVLRQAEALSASWLN"
     gene            complement(13306..14331)
                     /locus_tag="PK264_00320"
     CDS             complement(13306..14331)
                     /locus_tag="PK264_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HRD74373.1"
                     /translation="MEYFRQRFVEELNDEGLVTVRTFEWPPADVLKTMAPLDYEAHFL
                     DWVASQKQNAMDRAREFLERFNCLDRFNRLHTQLKRQSVIPFVGAGLSRPTGFPLWGA
                     FLAGLTADYPAIADDVRAHLAAYRYEEAAQLVLDRMGPDVFAEAVQNAFGSRVKALRG
                     PVQLLPLIFKRGCLTTNFDYVLNRVYEASDCRLKGEFSGTRLNEAPRRLADEPHCLLR
                     LHGEADSAQGRVLTGGEYTACYGDGGNYRELFKLLIANTSLLFLGCSLSVDRTVYALR
                     EIKQDAVVETPRHYAFLPLSEGMDREARRNELGQADIHPIWYPPEDHDQAIEDLLISL
                     MEGGFHD"
     gene            complement(14344..15567)
                     /locus_tag="PK264_00325"
     CDS             complement(14344..15567)
                     /locus_tag="PK264_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRD74374.1"
                     /translation="MPEIVPTALSEIVEIQQGRYLSKEEMAEQASADFPIPVHGANGI
                     IGWTSAPMYPEGVPLVTCRGSNSGMVLWAKGPLWVSNNAMAILPRAGSNRFLFYSLLN
                     SPPYSVVTGSAQPQITRRDLIGHRIAWASPLVQEAIASVLGALDDKIELNRRMNETLE
                     AMARAIFKDWFVDFGPTRAKIEGRAPYLAPDIWSLFPDRLDDEGKPEGWITSTIGQEV
                     EVLGGSTPSTKEPSFWGGDVAWATPKDLSSLSTPVLLSTERQITEAGLSQIGSGLLPA
                     GTVLLSSRAPIGYMAIAQIPVAVNQGFIAMICNKRLSNVFVWQWTQANMETVHQNANG
                     STFQEISKANFRPIEVTVPALELLRSFDETAKPLFDRIVANDKENRTLATTREFLLPK
                     LMSGEVRVGDAALSA"
     gene            complement(15560..17374)
                     /locus_tag="PK264_00330"
     CDS             complement(15560..17374)
                     /locus_tag="PK264_00330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRD74375.1"
                     /translation="MAEQAQKDRFVTVLKELGGSAGNGRLREALQWDEAAYNAVKDEL
                     VAEGIVTPGRGRGGSVSLRGAVEAEVEEAVPVATRAKPARANGKGGDLGFEADLFKAA
                     DKLRGNMEPSDYKHVALGLIFLKHISDSFEAKRAELLANYPDGAEDPDEYSAENVFWV
                     PKEARWSHLQANAKQPSIGKMIDEAMIAIEKRNETLKGVLPKDYARPALNAVMLGELI
                     DLISGISLGQEKGEARDVLGRVYEYFLGQFAGSEGKRGGEFYTPRSVVRVMVEMIEPY
                     KGRVYDPCCGSGGMFVQSEKFVELHGGRIGDIAVYGQESNYTTWRLCKMNLAVRGIDA
                     DIKWNSEGSFHKDELRDLKADFILANPPFNISDWGGDRLREDVRWRYGVPPAGNANFA
                     WVQHIVHHLSPSGVAGVVLANGSMSSTQNSEGEIRRALIEGVNGAPGVVDCMVALPGQ
                     LFYSTQIPVCLWFLARDKSNGIARNAKLRDRCGEILFIDARKLGHMVDRTRKEFSDGD
                     IEKITRAYHAWRGEGEAGAYEDVPGFCKAVTLEEIKAHGYVLTPGRYVGTADIEDDDV
                     PFAERLTGLKGKLELQFAESERLTGIIRARLKGVAPDA"
     gene            complement(17571..22259)
                     /locus_tag="PK264_00335"
     CDS             complement(17571..22259)
                     /locus_tag="PK264_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74376.1"
                     /translation="MTSNKSDAPDTDGSAKGTEARNHLLSKHVGHLRDALLSLEPAGP
                     AGFEGLLACVLSKASGQDFRLAKSGLQNGKDGATSSTASNHISFEGKLYNKKINDNEV
                     LTKITRLIGSGAPPDVWVFGATVEASTQLLDPMHTAAAKNGIGILVLDWPSASAIPPL
                     AAACALASEETSVFLRKHLNEVECVERAEAALAAIQRAETFRASADALVQKLQESSLG
                     QANARSANAKWLEAVFADRRRAKAAFGQVLAPNAEGALSLRQRSAIVEQVHQHLSAAP
                     SRKIVALVGGEGSGKSWLLAQSWLELGVRPLLVVIPATDLKPVAAYGSLTPFLTSKLI
                     QQTDDSDTEIPRKRWERRLARWKEQANEASPRFILCVDGLNQQPGFDWPRWLDDAAMV
                     VEQLGGTLTITSRESYFDERIRSAVHSKIATVRVPEWTERELKEILVAKGIDASKIKP
                     AVLTRLRNPRILGIAFDLLDKVEVKNFDELSVERLLFEHIRVGAHDGNAAEPPDQFSK
                     RLAQHAQDIIDRVKQQQREDRLIFDRADGKGGHALSADLLAVTAEHFFRPLPEDSTLY
                     ALADGGLSLALGLSIIKALQKAERNGRDVADALDELIEPIAALDKTADAVFSGVMVSS
                     IDEQCSLAIRRALICGFLRLQNVDAQNYPAFVAVARNATDAAMLALFDLSAANSRYAA
                     NKDWLSAALRKCRHKPECWAIISKHVSNWLRSYSLDPKIGVMSTPGHDSDDKVAKDTE
                     EEIQALARKMAELSAAERVFLERNMLQCKGYDPSLLHQDAFEMLAGMPLAGFAEQLVA
                     CSFSRALNSSFRAPYDEYLALIRYNRRDWQETRKLLLEASDFLAQKDTSQTGQWALVA
                     ILRGISTVEDAGRAEQLIEALTKDREKFGGWRLVEKYCATDPCDPASTKPDNLDVTAD
                     KYSQVDAEEVARHASMGEEDHFVRDALPGLVRFAPEVAIEAQRKIARSIVARESAELM
                     RGITLLEPHCAALDSETVGKLVEIANTLSSPREPESKDSHDNWIASQYAIQIAFPHLG
                     GDRQLDHLAALPSHGPPLLKLAEVLKPAMPEKLEWSLNQAMQSAEHNRALVALLFARC
                     SGTILTPRSRTLVGQFAENERSSVRAEAMDIIAHLKDRDLIERVIATGWSAALLDPRK
                     NHFEIWYGSLIVINAAEQGMISADEALDRTTPRLCSVAAEVLGAECHAAIAARLTAAA
                     AKAIDIELPFLPPSVEQDMEMAGRPIPPLLSLAEPDEVLGPEAFFKRLSETPEDFEAR
                     QRQGWEAYNRFETALTNEKARLILEDVGFEAVDACVSTSPDHCAALVGQLLGLDDRKL
                     MHVQNFALMLARSVSRHDLTLARKLFDRLSGRRAFVSLVSGLSGVSLEAICIWESADG
                     GALDELRAQRLDRAANDHQLAQEVLAALMAGEAAFLEQYARKNLERPEPAANARALMV
                     IGFGMESTTSEALLDRYADAKGLIGNAAKAARFAYDRNRWARHWFEKMCATNSPEEFW
                     AFSILFLKIVDARVGLWKTSVPRSGAAIGWFEPSIHSKLKHRAKAWKAKREKTLCGDK
                     MPSQVYTVLE"
     gene            22919..23257
                     /locus_tag="PK264_00340"
     CDS             22919..23257
                     /locus_tag="PK264_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74377.1"
                     /translation="MSKLVRIFAFVFLAAFAAGTAANAAAATQMSLMMSMANMDDGGM
                     ADCQDCPGDDAQTSACDQFCVTTLAAICTPAGTVLPNVTDVVVIGTAASPGGRVGPPD
                     PYPPRTIHMG"
     gene            23351..24814
                     /locus_tag="PK264_00345"
     CDS             23351..24814
                     /locus_tag="PK264_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HRD74378.1"
                     /translation="MSYPRLPALTRRAFLASAAVVSASTILRSQARATSVRAFHLRAG
                     YGRAQIAPEPYGQTPVWCYDGSLPGPEIRVRQGERLRIAVANALDEETTVHWHGVRVP
                     NAMDGVPHLTQAPIAPGEVFVYEFDAVDAGTFWYHPHHRSFEQVGRGLYGPMIVEEAV
                     PVQVDRDLTWLLSDWRLTRSAEVRDDFGNRHDMMHNGRVGNTVTINGRVPDTFPVRKG
                     ERIRLRLINAANARIFGLDFGSHEPVVIALDGQPVEPHAPEGRLVVLGPAMRADVILD
                     MTADAGSQVSVVDRFYEGLEYRLVDLAYEKQPLRDTAPDWPLALPPNPLPEPELATAA
                     RHEVTFNGGMMGAMVMQEMGGSMGEAAPGGMMGGMGSMMNMMHSGGIWFVNGVAAEGH
                     IMDPILTLARDKSHVIAMTNATAWHHPIHLHGHSFRVISRNGKPTRHREWQDTVLMSP
                     RERVEIAFVADNVGDWMLHCHVLEHQAAGMMGVFRVA"
     gene            24841..25281
                     /locus_tag="PK264_00350"
     CDS             24841..25281
                     /locus_tag="PK264_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HRD74379.1"
                     /translation="MRTILSAVALASFVWGTAPALAGEKDVTLYKNPQCGCCEGYADY
                     LRDNGFTVTTKPTHDLAEMSRQAGIPDEFQGCHLSFIDGYVVSGHVPIDTVEKLLTER
                     PDIAGVTLPGMPLGSPGMSGVKEAPFEMLEITTSGTIGGVYARE"
     gene            25313..25450
                     /locus_tag="PK264_00355"
     CDS             25313..25450
                     /locus_tag="PK264_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74380.1"
                     /translation="MMDGGMMDGMSGMMLGMGLGWLLLIVILVLSAAALVKYLFFSGR
                     K"
     gene            25447..25902
                     /locus_tag="PK264_00360"
     CDS             25447..25902
                     /locus_tag="PK264_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRD74381.1"
                     /translation="MRSTPILAVMAMLAGAAVMAWVWLFGAGSGEAAGIAQGRQLYAE
                     HCAVCHGANLEGQSDWKRPLPSGRMPAPPHDASGHTWHHPDGVLFRITKEGPAAVVGD
                     GYESDMPGFGNMMSDEEIRAVLAFVKSTWPERERAYQTEMSRREKDEAR"
     gene            complement(26135..26728)
                     /locus_tag="PK264_00365"
     CDS             complement(26135..26728)
                     /locus_tag="PK264_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD74382.1"
                     /translation="MSPIETVCSAIVLVYMALFLILTARAARASGQSFWLFGKGSESQ
                     ALPALLFRLSFVGVAFYALTALVLGDPLAAPVHNALDGRFGDLVGAALASAGAGLALY
                     SQIHMGRSWRIGAASGQLGTIVRTGPFAFSRNPVFVGQVMLFLGLALALPNLIQFTLS
                     FLLLAAVHRQVRIEERVLEGDLGAEYQTYRREVRRWI"
     gene            complement(26889..27515)
                     /locus_tag="PK264_00370"
     CDS             complement(26889..27515)
                     /locus_tag="PK264_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRD74383.1"
                     /translation="MKALKIIRITAWGAVAIVGGVLLAFSGILPGVEKPGALTSSTVT
                     GVAAIGGPFELTTHGGETLTDEDLEGKPYLVFFGFTHCPDICPTTLFELSGLLADLGP
                     DADKIKPLFITVDPERDTQELLASYMTSFDTRILALRGTKEQTDQAAKAFAAYYEKVP
                     LEGGNYTMDHTAGVYLMDAEGEFMGTLDLHEPRETQLRKLRRLADTAA"
     gene            complement(27512..28015)
                     /locus_tag="PK264_00375"
     CDS             complement(27512..28015)
                     /locus_tag="PK264_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRD74384.1"
                     /translation="MKLVKTLALGLVLSAATQGVLAHDYEAGPLAIDHPWARATPPGA
                     PVAGGYLTITNTGTEPDRLLGGSSDAAEAVEIHESTITDGVARMRPAEDGIAIAPGET
                     LRLEPGAAHIMFVDPAKPLKDGERFPATLTFEKAGDVAVEFAIVPMGATPEAPQNHEG
                     HGAPVSQ"
     gene            complement(28012..30219)
                     /locus_tag="PK264_00380"
     CDS             complement(28012..30219)
                     /locus_tag="PK264_00380"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRD74385.1"
                     /translation="MAVAPASALKLRVEGMDCGACGLKIENGLKRLPGVSDINVNVGL
                     EMLSLVLDEDRTSRTVIEERIRSLGYTPYPVGGEPMADENVPPDRPETVDRTWWRTGK
                     GQLVLGTGALLALAFAVATIAPDVADWAYVAAAGLGLVPIARRAVAGARHGTPFSIET
                     LMSVAAIGAIAIGEGAEAAVVVFLFAVGELLENVAAGRARSGIASLMNLVPRVARLER
                     DGTVAEVPVERLAIGNIVVVRPGDRVPSDGEVVEGSSELDEAPVTGESVPVLKEPGSA
                     VFAGSINANGVLRVRITRTAADNTIARIIHLVEEAQGSKAPTARFIDRFSTYYTPAAM
                     AVAALIMIAPPLLFGAEWSTWVYRGLATLLIACPCALVISTPAAIASGLAAGARRGLL
                     VKGGAALETLGKVRTVAFDKTGTLTVGRPRVTDVIALEGDDAEILAKAAAVESGSSHP
                     LGAAIVEAAEARGLELPKTFGGAMAVPGKAVTARLREGFVSVGSPRYAAEQAEVPEEI
                     GARLRSLEFEGKTAVVLLSGKRILGIIALRDEPREDAAAGVARLNELGVRAVMLTGDN
                     ARTAAAIAGGLGMEAKTELLPDAKLSEIGALRAHGGVAMVGDGINDAPALAAASVGIA
                     MGGGTDVALETADAALLKNRVTGVAELVELSRATLGNIWQNIALALGLKAVFLVTTLT
                     GTTTLWMAILADTGATVLVTINALRLLRFRANRASTSGRGASAAKNERIGVTG"
     gene            30287..30700
                     /locus_tag="PK264_00385"
     CDS             30287..30700
                     /locus_tag="PK264_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD74386.1"
                     /translation="MTREKFSIGDLAKATDTKVETIRYYERIGLLPEPARTSGNYRSY
                     SAAHLGRLSFIRRARDLGFSIEQVRDLLGLSDQRDRPCEAVDAIAREHLGEVQRKIRD
                     LQALRRELDSILSQCGCGTIAECRIIEALSPNDHR"
     gene            complement(30731..31066)
                     /locus_tag="PK264_00390"
     CDS             complement(30731..31066)
                     /locus_tag="PK264_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD74387.1"
                     /translation="MNGSCSSGRRVILDLYGTNHDARTWDSPEAFWPERFRAWDGSPF
                     NFVPQGGGDHNADHRCAGEWITIQLMKQASDVLTRRIRYQVPEQDLRIDYSRLPALPQ
                     SRFIISRVS"
     gene            complement(31063..32005)
                     /locus_tag="PK264_00395"
     CDS             complement(join(31063..31668,31670..32005))
                     /locus_tag="PK264_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HRD74388.1"
                     /translation="MVRALSEDLRKRVVRRVEAGHSCREVAAIFGVSVASVVRWSQRK
                     RQTGTVAAKPMGTRHARSLAGQRDWMLQRLQAKPDLTLRALVGELHARAIPASYGSVW
                     RLLDDEGITFKKSLHASEQDRPDVARRRERWKRHQGKLDPARLVFIDETWAKTNMTRT
                     HGRCARGQRLVAKVPHGHWRTLTFLAALRTDRIDAPCVMDGPINGQSFLAYVEQILVP
                     TLTPGDIVILDNLGSHKGKAVRAALRRAGAKLLFLPPYSPDLNPIEQVFAKLKTLLRK
                     ADRRSVEATWRHIGSLLNNFTPTECSNYLSNSGYASI"
     gene            complement(32086..33024)
                     /locus_tag="PK264_00400"
     CDS             complement(32086..33024)
                     /locus_tag="PK264_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD74389.1"
                     /translation="MMPQLPRDNRLDATLALLKDPYRFISKQCRAHGADLFETRLMLG
                     KTICMTGPEAARLFYDQSRFKRQGAMIGRIQKTLLGRGGVQGLDDAAHRHRKQMFMSL
                     MTPERIEGLIATTAEEWQARARLWAQKDEIVLYDELHELLTRAACAWAGVPLPEAQVP
                     QRTHEITALFDAAGSVGPKHWRARWARKRANRWIENVVMQIRSGQMRPPEGSAASTIA
                     KHRDLDGELLSPHVAAVEVLNVIRPIVAVGVYITFVAHALHHHPDCRSELEDGDDGSY
                     PELFVQEVRRFYPFFPAVAALVRSDFDWMGYRFPRA"
     gene            complement(33021..33521)
                     /gene="lspA"
                     /locus_tag="PK264_00405"
     CDS             complement(33021..33521)
                     /gene="lspA"
                     /locus_tag="PK264_00405"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRD74390.1"
                     /translation="MLKLGLPIAFIAFTADQVSKWLILNHVMAPPRVIPVTGFFNLVL
                     GRNTGVSFGLFGDASPWALMALTLAIVAGLIVWMQRADYRRTAISLGLIAGGAVGNLV
                     DRLRHGAVTDFLDFYLATFHWPAFNLADVAIVSGVGLLLLESILPRDEAKGPKKHPDV
                     GPMKAP"
     gene            complement(33546..34289)
                     /locus_tag="PK264_00410"
     CDS             complement(33546..34289)
                     /locus_tag="PK264_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRD74391.1"
                     /translation="MDPSGVGIATAFAAGVISFLSPCVLPLVPGYVSYVAGRTISPDG
                     LAGPVGLKETVRTLGLGLCFVLGFTTVFVLLGASATALSGLLRAHLYEANLIGGAIVI
                     VFGLFTTGLVPMPWFERDVRFHSGRHGGGPWANYLLGLAFAFGWTPCIGPVLGAILAL
                     SAANATAGSGTALLAVYSLGLGLPFILAALFMRGFMARMTAMRRTGRVLKIVAGGTMV
                     VMGIAMITGQLSSFAIWLLQTFPALGQIG"
     gene            complement(34305..35189)
                     /locus_tag="PK264_00415"
     CDS             complement(34305..35189)
                     /locus_tag="PK264_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRD74392.1"
                     /translation="MTAPATARYHVTGMDCASCAAKIEGAARKVSGVSDVKVSIASQI
                     MTLEVDDPAARLPVVENAVSELGYQLDRIGAAKASADAAEDDDDDRIPDLSHVTPAYK
                     RALWIVVLLNVGYGVIEIGGSFLAGSQALQADALDFIGDGLISFLGLIAVGWGLAARA
                     RAALLQGIFLALLGFGVIGSTFYRVFVEHEPQTLLMAGFAIVALIVNVLAAVVLLPHR
                     KGDANMRAVWLFSRNDAIGNLAVVVAAGVVWALNSPWPDLIVAFAVAGLFLQSAWSII
                     RDARNDLREAASASGAKA"
     gene            complement(35186..36556)
                     /locus_tag="PK264_00420"
     CDS             complement(35186..36556)
                     /locus_tag="PK264_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HRD74393.1"
                     /translation="MGENLLGLVIDNRRARKEGAEGIVRRQRERLGAMVAFARANSPL
                     YRRLYADIPEGPVRLENMPITTKKWLMSGFDDWVTDRTIRLSDAQAFAEDLKKVGEKF
                     HGYTLATTSGTSGTKGIFVYDDRAMAVTQSIAFRMLTSWLSFGDFVKIIAGSGRMAMV
                     MAKGGHFASAVAAAALKRRRKNKLLQLSVHDPIREIVAALNDFQPVLVAPYASIAALL
                     ATEQEQGRLHIRPALLALAAEGLAPEEYGRIARAFNAKVGNSYASTEVPFLSFSCPHG
                     WLHVNSDWATFEAVDASHRPVPKGDVSHTVLVSNLANALQPVLRYDLGDSIVERPDAC
                     PCGSPFPAIRVQGRASELVRFAGRGGQAVTMPPMVFYSLLDPVQGVDLYQVVQTAPDA
                     VRVRLKVSGNPDTVWPRVEADMRGLLERHGLGHVRIERGDEPPEQTQGGKVRPIIPFE
                     PKGPNP"
     gene            36653..37039
                     /locus_tag="PK264_00425"
     CDS             36653..37039
                     /locus_tag="PK264_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD74394.1"
                     /translation="MQIGDLSRAAGVNIETIRYYERIGVLPRAARQVNGRRTYSQDDA
                     RRLGFIRHARDLGFDLADVRTLLALQEQPEASCEDASRIAQAQLEAVESRIARLQSLR
                     AELTRMLTECQNGVVAQCRVIEALNG"
     gene            complement(37522..38196)
                     /locus_tag="PK264_00430"
     CDS             complement(37522..38196)
                     /locus_tag="PK264_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HRD74395.1"
                     /translation="MTTSYFNHWRDVPEGAWRWKNFSPAEIACRGSGSLRINEGALDK
                     LQALRDRLGKPLIVRSAYRSPAHNRAVGGAPRSKHMDGTAFDIAMANHDPVAFEVAAR
                     AVGFLGFGYYPRSGFMHIDLGPARQWGARFPVQAMAFAAETAPVREVLADSRTMKGSG
                     AAGVATLGAAGVEVAQSILTETQSAILPLLPYLDTLRWVFIAVALIGIAVAIYARIDD
                     WKRGRR"
     gene            complement(38270..38533)
                     /locus_tag="PK264_00435"
     CDS             complement(38270..38533)
                     /locus_tag="PK264_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6127 family protein"
                     /protein_id="HRD74396.1"
                     /translation="MVSLPSDEFEALLERAAETGARRALHEVGLDGADAAEDIRDLRS
                     LLSSFRLAKQTAVQTAVRLITTGILLALMAGIAIKLKLFGPSP"
     gene            complement(38661..38864)
                     /locus_tag="PK264_00440"
     CDS             complement(38661..38864)
                     /locus_tag="PK264_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74397.1"
                     /translation="MDLTWCVSAIQVPALGGLSWLLQSHRAANERDLKARRDELAAFK
                     LHVATSYASIVYLKTVFEPGDIL"
     gene            complement(38910..39641)
                     /locus_tag="PK264_00445"
     CDS             complement(38910..39641)
                     /locus_tag="PK264_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74398.1"
                     /translation="MPTYPKRLAVTNPSFETGVAPGDGWTTVNGTPVFKTTSPPTDIR
                     GGSRYLQVANNGSAGSYRVRQDVVVPADCLTDVDAGLLSVSLRVLWSNFAAGNDIGFV
                     EVAFLDVADVLISTASSGNFPDSAQAWGLRALTPAIPAGTRKLRLILGGMWVTGTSTD
                     SYFDIVDAELVLSTPPAGDLVQVYAEALLDAPSPARAAQAYAEALVATASTGRAAQIY
                     AELIRSVADKPATAGGGTIVCVVTG"
     gene            complement(39648..40688)
                     /locus_tag="PK264_00450"
     CDS             complement(39648..40688)
                     /locus_tag="PK264_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74399.1"
                     /translation="MALQFCDGFDSYGAIGDTVGKWGSYRFSVAADLSFLPTGGRFGG
                     GAVRFSATTGMLVRKTNPAVDEMFVWAAIFPRSYPTTSTPFLWFEDVLGNGASLRITT
                     GGALFGTLMGSSAAFTSNSANSIVLGEWNTIALHFKCADSGGRWRLILNGVEQFNLIG
                     DTRSGTSGPNIDRVIIGTPRGGAGLEWDWDDFILHDASGSYLNAILTSDLKIETLRPT
                     ADQPSGGWTPSTGTDRFALIDEAAPNDADFLSATATGAREIFTMTDPVAEPQTTHALV
                     VNVRGRRTDVGARVLKPFIRVNAAEDLNAGLALSNQFSTLQHPVYRNPDGGGPWAGST
                     INAAQLGIELGA"
     gene            complement(40708..41427)
                     /locus_tag="PK264_00455"
     CDS             complement(40708..41427)
                     /locus_tag="PK264_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74400.1"
                     /translation="MPTPNLAITHVAASQNQKEVTINDALDRLDLAMNDTVDVDCTGG
                     NTTVSASDYRENFLIRLTGTPASDFTLTVPDGKRILAVHNTTARIATLRTTTAGATVI
                     LRASELGIVGSRGTNLVSLAASAIGGLYDLGLFIPGLPAATALVFQYVFPRGVSFPTS
                     LTGSTGRAATGATSLASFALRKNGTNVGSVDFAAGQAVASFTLAGGTNFAAGDVLELL
                     APAPQDATLADISLSFVGART"
     gene            complement(41428..44934)
                     /locus_tag="PK264_00460"
     CDS             complement(41428..44934)
                     /locus_tag="PK264_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRD74401.1"
                     /translation="MAILVAVGGAVLGSAVGVGWSAGWLVGSVVGNLIFPRRGPDVRT
                     EGPRLGDLTVTSSAYGAPIAIAYGTLRMAGNVIWSSGIREQQNVTRTRSGGKGGSGGA
                     TQTSVTYSYFASFALSFGEGPAEDVLRIWADGKLIYDKTGSSADVAKPNLRFRFHAGE
                     ETQLPDPLIETHVGAGRAPAHRGLCTIVFEDLALADFGNRIPNITAEITFRRAESRPY
                     QLLDFITTGEGGLLDSYQIDELAVDWRRGYGYFIDSDVSASNAGIRRFSLRTMKEDRQ
                     ARMSDVTSVSPNNFPSTLFCGEDGYLYLVTGSSNSRPILRVEPNALKEVSRFGSTSSG
                     LSNSTLRFVATTWMGMVSAYGPSGRADFILTGSLFDDVGLIRADGMGYVWGAGQTVDE
                     SRVRGAIGGAVGEGFGEGWVLGSGTGTSHTGLALYRIRVAAAAAYDSLSGLSLGVTFE
                     KVASFAPSQIQAGATAFYDDAGGLTYDATDDGVIFQVQISNGGSAGAIYTVKWRSDAG
                     IVWKTPTPIQINYEGPFFGQSRLQGQHWTLMRSTRLVQIDTATGALVTDETWPGAVSE
                     TGAQVYDAITDTHLVRGANGWARLFLNRGGGEGETLSGIVADLCERAGLGLADLDLGE
                     LDDIVPGYVIGRQTTVRGAIEPLAQAYFFDAAESDDVLRFRTRGRAPVATIDSQFLVP
                     IDDRTAETWRERRTQEVELPERVSIVYMDTAADYAQGSQSEKRASLPTPTMHSRNQAS
                     LELALAIDATTAKRIAAKTLFSAWEERSSYEAQLPPDWLRLDPTDVIDVVFATGSGFR
                     TRLTRIDVGADFLLGIKGVSETAATYVSSVLADSGAGKPAQVIAAQAASKLILPDLPL
                     LRDTDDLGQTGSRAYYLMGGFGGPGWPGGSLYRSLDGSAWSQVGRALSEAAWGATANA
                     LGSPSSPFATDEVNVLAVFMTTGSDRLESVSQEALVNGANAALVLKANGEPEVIQFRD
                     VTLNPDGSYTLRGLLRGRRGTDVFVSGHAPGELFVLLDPDDVESLSLSLGDLGLSRLW
                     RALGFGTLFEDAETVSHAHTGRDLKPYAPWNLRAVKTGSPSNITLSWVRRTRIGGELK
                     DGSGVVPLGEASEAYEIDILAGPGGALKRTLVTASPSVVYANADILTDFGTVPTSLSV
                     AAYQLSAIAGRGFARAVTVEVA"
     gene            complement(44938..45372)
                     /locus_tag="PK264_00465"
     CDS             complement(44938..45372)
                     /locus_tag="PK264_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRD74402.1"
                     /translation="MHDETVTADRLVAQARSYLGIPWRHQGRTRAGIDCAGLVVLVAR
                     DLGLADYDSTAYGRRAQGQGFVEHFRSQMDSVAVTEVRGGDVLVFADQAYPCHCGFLT
                     ERFGTPHLLHAHALRRQVIEEPYASEWRAKVKFAFRFRGVSE"
     gene            complement(45362..46942)
                     /locus_tag="PK264_00470"
     CDS             complement(45362..46942)
                     /locus_tag="PK264_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HRD74403.1"
                     /translation="MKSVSTALAAHLAGPVTTLACLWRITRLDGVEFYFTDHDRDIDF
                     EGSTYRASTGYSRTAIANDASLSVDNLDVEGVFDSTAIREEELRAGLFDGAEVRIFLV
                     NWMDPAQGPLRMRRGWFGEVLLTEQGTFRTELRGMTQALQQRIGELYSPECRADLGDH
                     RCKVPILPAEIARATAYAAGDVVRVRTSTAPAQIGNPFVNPGFDAGSLSGWTVASGTA
                     TAKTISSPLGPKTRTHFLEGGSIASFEVRQTVDLTDVIDPAKTDSGAYLVTLRGWRAN
                     GSNDLVDQGRLRFECLDAAGAVLATPLDTGNEVAGTSWSLRQAVDVPVPAGTRQLRVI
                     FNGTRLGGTVCNAALDGIEGHFSDTTAVVATSAAFENRIYRCTTAGTTAVTQPAYTTT
                     VGAQTTDGTAVFIAEDAWTRSGIVADVIDRASMFVVLDEPRAVDDWFAGGVLTWESGN
                     NAGRAMEVKAWTASTARLELFLAMGYAIRVGDAFRIHPGCDKRLDTCIDRFANVLNFR
                     GEPYVPGQDSLMTYPDAR"
     gene            complement(46939..47541)
                     /locus_tag="PK264_00475"
     CDS             complement(46939..47541)
                     /locus_tag="PK264_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HRD74404.1"
                     /translation="MAGFHEVQFPPDISYGAAGGPGYSTTVVTTVSGHERRNANWAQA
                     RGRWNVAHGLKKRDQVAALIAFFRARKGRAYGFRFKDWTDYQGLAQAIGVGDGTTKTF
                     QLVKHYASGAEVEQRTIVKLVAGSTKLYRNGIEATSGWSIDITTGLVTFTAAPASGVQ
                     VTADFEFDVPARFDSDQMDITIETYQLGSWGQIPIIEIRP"
     gene            complement(47554..49998)
                     /locus_tag="PK264_00480"
     CDS             complement(47554..49998)
                     /locus_tag="PK264_00480"
                     /inference="COORDINATES: protein motif:HMM:NF018497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HRD74405.1"
                     /translation="MAERNLSVRLAVIDGGKVKAELRDVGETGERALKRIEDASRPAS
                     RALQAIDGATGQVRGSLEAMTGRLGPLGAALTRLGPAGIAAGAALAGIGLALTAGLQE
                     AAEAERSYRRLEAVLRATGQASGLTARSIAAFADEIERTTLTTAESVQDAAAVLATFR
                     SVSGDTFTRALRLAQDLSAVFGQDLRSSATQLGKALEEPVEGISALRRVGVSFTASQR
                     ELIQSLVETGQTAEAQKVILDALEQQVGGAGAAEAGGLTGAANRLSDAWGNLLEAIGQ
                     TPAVSGLAEGALNLLADAAEGIASLFEDDPIATRIVALNKRLIEAQDELARLQAGGPG
                     TPLLGQRFAIEEQRRTVEALQRQVDDLIAQARSEAESFAAEQRQAEAGRRAAEAERLA
                     ELLATQRRDIDRVIDQIATDPGERIARINRELEETRRRLTALRLPDGSNASDVDAAIA
                     RAEELARRRIDAIERPAREAATRAGAANARIIEDFRRQVAGLNDERQAAIDQALSRLS
                     EGATAAQRAEVERLAGALYDEKQAREELAKAMREEERLREEGRRLIEQTRTPTEEYAA
                     TLERLNTLLRAGAIDQETFNRALAGANEDLAEAQERALRQSREWQDGVRRALKDYVDA
                     STDAASAAEEATTLAFQSMEDALVSFVTTGKFEFSSLTESILADITRIAVRQAITAPL
                     AGFLNDNADDLLGGIGQIFAGLFHEGGVIGHSASPARSVDAGLFLTAPRYHTGGLAGL
                     APDELPAILKRGEAVLTPEQMRALGGSMGRESRASQPINVVMNISTPDTNGFRYAQGQ
                     IAAEAARAIDRARCNL"
     gene            50089..51132
                     /locus_tag="PK264_00485"
     CDS             50089..51132
                     /locus_tag="PK264_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaC family protein"
                     /protein_id="HRD74406.1"
                     /translation="MPAPELRIGNRTAYLRKAIRTPDFQSESVLTRDAFQFAELWLRR
                     NCREALPFWRQAANYYSASRNLPAVSAPLTSYYCFLNATKALLTVKGVAFSERHGVSG
                     EFNPAAKRVLSNEVVKIQSAGIMTALSSYMEEPETQNEHALTDLLANLPFIHRSYRHT
                     FKSQKELFIPVRNVVYRKHPTDDYVWFTAEIVGRFADKRSLRTLPPRFEVDAGYDDRC
                     VIRTKKRIKWFRRGTPKASKAQALARLNTLHRSVRLDLSFISAPLDLWYLRREVSGNS
                     IIRRYGMTMIMAIMHRLSELSRYDPKGLSGHLDGQANWLLTEFIQLAPAQFIDEIICE
                     MTSLEFRLPGVRP"
     gene            complement(51510..51872)
                     /locus_tag="PK264_00490"
     CDS             complement(51510..51872)
                     /locus_tag="PK264_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74407.1"
                     /translation="MIRLDLKREPHWLDLGHGVRVHVRPCSTALVMAARAAAARANVG
                     VPIDDEASVGLRTAALVKALARLAVDDWEGVGDTEGKPVAVTPEGLDALLDLWPIAEA
                     FERNYLGPALLLDAEKNV"
     gene            complement(51881..53158)
                     /locus_tag="PK264_00495"
     CDS             complement(51881..53158)
                     /locus_tag="PK264_00495"
                     /inference="COORDINATES: protein motif:HMM:NF039819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HRD74408.1"
                     /translation="MAKVRAYGADATLKVAREVSYGVAPLAGYRSLDFKSTDLSSNQP
                     LGDDPLLGRGRNAQDPYRGLITDEGQIEIPLDLRGTGFWLTALFGDPTTTTVAASGSI
                     AFAGQPTATSTITINGVVWTFVSGTPSGNQIEIGGTLAATLTNAAAALNASASPLVSA
                     ATYGTSGGTTLTITHDTAGPTGNAFTLAASATSNGTVSGTTLKGGGYAHVWDSGADDI
                     PSFTFEVGHPKLVTPVFFRHLGTVVENLTFEMGQEGPANARLQLVAQGEEGAAATIDA
                     TPDSFALKRFSQGRGFIRRGGSALAGVTGGTLTFSNNLERVRVIREDGKIEAADPTFA
                     SCQGGMTVRFDGATLVAEAANGDPVVVDYGFTMAEGWELKFELFRVFLPKPKYAVSGP
                     GGVEASFDWRAAFDETQGTMLRARLLNDVASYV"
     gene            complement(53186..53617)
                     /locus_tag="PK264_00500"
     CDS             complement(53186..53617)
                     /locus_tag="PK264_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74409.1"
                     /translation="MSRREAILSALFERLQTLLSATVRRNETLPEKVPVAGLVILRDG
                     EPGEPDVTLNPRTEFYSHRIEIETYVTRAADGSGEAALDDLLGDIGLALATDPSMGGL
                     AENLNTSAPEVSALAIEGAAPILSARLTITIEYLVSDPLTS"
     gene            complement(53617..54282)
                     /locus_tag="PK264_00505"
     CDS             complement(53617..54282)
                     /locus_tag="PK264_00505"
                     /inference="COORDINATES: protein motif:HMM:NF040424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6441 family protein"
                     /protein_id="HRD74410.1"
                     /translation="MRLSAALSGSLKQLMEAELKGAERAITLGVREATDGLKGELRTQ
                     ITGAGLGEKLARTWRGEVYPKGQSSVAAAGFVWSKAPGIVRIYEDGATIRSTTGLFLA
                     IPTLAAGRYGDGGAKITPGGWERRTGMRLRFVYRRGAPSLLVADNSRLNSRGRAVANQ
                     GRRQGSTFTRLSGRSTVPIFVLVPSVTFKKRLDFAGAARRWHGRLPELVLRQWRLTEG
                     STR"
     gene            complement(54279..>54537)
                     /locus_tag="PK264_00510"
     CDS             complement(54279..>54537)
                     /locus_tag="PK264_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013654507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74411.1"
                     /translation="DAIWRVAGAGSGVAIRAVVRRPEAISAFGDTRLVTPVALVDLRV
                     SEAPTIAAGDTIEIDGDILLVQGEPVRDAERLIWTVEGRSP"
BASE COUNT        10525 a        17086 c        16823 g        10103 t
ORIGIN      
        1 cgaggcggcc tgctctgtcc cacgtatagt catagtccag cccacccggc agaccactgg
       61 caaagcgtat gtcctgagac accaccgccc cgaactggtc ataagtgaac gccaccgttg
      121 acacattgcc ccccgcgcca gaaccggcag cccccgtgcc cccgcatccg cgccatagcg
      181 cggagaggcg agattggtga ccggagatca gtagagtctt ccattcaggg catttgctcc
      241 aagagcaacc gcccgcaggg tttttccgga agctttaagt attggctaag catgaagctg
      301 gcaatttcga caccattctc gctggccttc tttagccaga acaatccccg cctattcaac
      361 tcagtatcta actcttcgca ataaatgaca ccgattgctg acatggcgcc ggcctcacca
      421 ttctcagccg aaatcaatat ccattttttt gccatttcag tgttgaacat gcagagatag
      481 tagtgattgg ctacagccgc gcttgccatg atgtcaccgc tgagcccatt agggattgcg
      541 gcctccaacg cctctgctga aagacaaaag cgcgcattca tgccagcttg ttcttcggga
      601 gccttctgct ctggcacgtc tgcaaatgcg cacacaagag atgaagccca aagcagtgct
      661 gaaagaaacg tctgtccaca cattttcatt ttgatctcct cagcgtggat agtagattcg
      721 ccattctcca cccggatcca gccagaaatt gccattttcg tagttgaggt gcacaagact
      781 cgttgccgct accacgggat gccctgatgc atctgcaagg gcttgagcat attcaagccc
      841 ggtcggagta gttccaccgc cgtagcagct caggagccga attgtctggc cgggtttgag
      901 attcgccaac gctgccgagt agtgatagtt gacttccaag tacggcagca agctctcggg
      961 gctgatcggc gtttgcgtac caaagtttgc gcgcgagtca actatcccgc cgcttatgac
     1021 accgtgcccg gcaatgacaa actcatcctg tgggaacaat gccttgctcg cgccgttcgt
     1081 taaacccgta cctccaatgt ccattattac atcagcacca cgcttgtagt tgctcccaac
     1141 tggcgcatat atctcgcccg atgttacaga cgtgctggaa atattcgcag cgggacccgc
     1201 aagcaatcct ctgtctagag tccctgtcgc cgcttgctcc accttaccac tcccaccgcc
     1261 gaaccctgct gagcttgcga ggccgatcgc acttcggatg atgccgaaca cgcggcccgc
     1321 gccgggatat gcatcttcca gcgccatcgt aattgcggtg tcgatggcat agttgatggc
     1381 ataattgacc agcaatcccg caaacgcgct ggctgacagt tcgcccgccg ccagctggag
     1441 caggggcacg atgaactgga agaatccccc gctcggatcc gtccccgcca tggggttgcc
     1501 catgccgtag gtatagagat tgagcgattg cttgtagccg accgggtcca cctgcatgaa
     1561 ccggccgatg agcggcatgt aggtgcgggc gcgggcataa tagatgccga tctcgcggtc
     1621 atattgatgg ccggtatagc cgaagatggt ggggatggag cccgtggtca ccccataggg
     1681 cgagtatttc gcctggccgg tgatcgcgcc atcattgctg gccgtggcga tgatggagcc
     1741 aaggcgatcc gcgtgcagga aggttttggc acccgtggcg cgctccacca ccaggaaggg
     1801 cctggagcga accttagaga gcgaccgctt cactcaataa tcgcgccggg cgcatagatg
     1861 aaccgccgaa gcagggcaga aacatcccac aggcccgaaa actccgcgac cgtgttctcg
     1921 cccgcgaaac tattcaatag tcaaactcgg gaaaaccagc atagcctcat catcgacgca
     1981 tgcattggca tacaggataa cgctcaagcg cagtttgccg tccgcaggat tccaccaagc
     2041 gccgccaaac ttcccaaaac tcacatacgg caggccctcc cgagtctcaa cctcgccgat
     2101 cagggttatc gtcagtttgc cttcgactcg cgtaagggaa tagtctggga aggtcattcg
     2161 gtcggcgctc aagacgcgat tgtatacctc ccatccaaaa taggatttca tatcgcggtt
     2221 gctgacatca agattttcaa ggagatacgc ttcatcgaga tcaaatgggg cgttctttat
     2281 ttcgagaaca acctccattt tgtcaccact tgtataattt cccgttttca agactaagct
     2341 cgaaccttct agaccagtaa tcgctgggca ttcttgtctg caaccggctg ccaacacagt
     2401 cgcgagcacg acgaatgcca gactgcggac cctcctttgt gtaccgatca tcggtaacct
     2461 ccgctacaaa tgacactgcc gttgccgcaa agaatggctg ccttccagcg cgggtacacc
     2521 tcaatgccgg tcagatcaag gtagtcaacg atcttggctc cagggcctgt gaacacgtcc
     2581 catttgtaag tcaggtccgc ccatgcaccc accctgtttg agaaaatgtc ttctgccatg
     2641 aaagcggaat ccagttgttg ctgaagttga tcgagctcga ttgctcccat catcaattcg
     2701 ctcgatgggc cagctccaaa cgcctcatat gccgagacag cgtgttgaag atcgaaccat
     2761 ccttcagaag tgagtgtgta gcgggagtcg cgtggcaccc ggagtcctgc catcaggctg
     2821 atgtcttcag tgtaggatga ctcatcggca gccttgtcta tcactgatgc caaattctga
     2881 ggttcgacgt atgaacttag tcggctgcct tcagtgaggt atggtacgat tgcagcggcg
     2941 cccgctttaa cggcaccgcc tgcaaattct accacctttc ccggccccga ccctgcaaac
     3001 accgactggc caaggccata gatagacaag gccgtgccga cggcggttcc cgctccggga
     3061 gagagatagt caaggccgcg cagtagccct tcggtcagca cagtttcgat cagcgtgctg
     3121 acaaacgcac ttgcgctgat ctcgcctaac aagaactgtg cgaacgggat gatcagtccg
     3181 atgaacgctc cactgggatc ggtgtgggcg acgggatttc cgaggccata ggtatagaga
     3241 ttgagcgact gcttgtagcc aacggggtcc acctgcatga accggcccag catcggcatg
     3301 taggtgcggg cgcgggcata atagatgccg atctcgcggt catattgatg gccggtatag
     3361 ccgaagatcg tggggatcga tccggcagtc accccataag gcgagtattt cgcctggccg
     3421 gtgatcgcgc catcattgct ggcggtggcg atgatggagc caaggcgatc cgcatgcaga
     3481 aaggtctttg cacctgtggc gcgttccacc accaggaagg gcgtcgaggc ccccggcgca
     3541 tagatgaacc gccgcagcag ggccgagaca tcccataggc ccgagaactc cgcgaccgtg
     3601 ttctcgccat ccgcgaagaa gaagctggtg cgcgtgcccg ccgggccgct gatcgtcagc
     3661 cggtcgcgcc tgcccgccgg gtcataaccc atggtggcgg aaccgttcat gcccagccca
     3721 atcttcggaa gaggggccgc ccagaccacc gccgaggtca tgcggaaaat gtcaggtgat
     3781 tgccgttctt gtacagcttt tgttcgcacc accccggagc cctatcaata tttcataaag
     3841 gtaaaaataa ccgctatcat gaccaacaca agagataata taaatatcac tctgaacacg
     3901 taaatactta taagaataaa accatcagtt tctctgatta tttttctcga aagcaaaatg
     3961 aaccgataaa ctcgaagcga acccgaccaa ttgtaaacaa aatatctacc gaggctgcgc
     4021 tcctgtcgga actcaggatg aacaatccaa atgatgaggt ctacaaagaa accaaatacc
     4081 attgttccac aaagtaaagc aacagaccac acaacagaat cgaattccat tttattcatc
     4141 ctccatatta cttaaagacg ttctttttat acacgccgag aaaagtacta taaccagccg
     4201 ttggtccgtc aacgctcagc gagatgtagt atgctgaagt gtcgggattg tagtaaaatg
     4261 tcccggtgaa cgaaacgggc aggatcgggt gaatttggcc agtgaccgtg gcagcaagcc
     4321 cccaaccaga agggtcgcgc ctctgtgcct cgaaaagtgt aattgagcct gcaaagtctg
     4381 gcttcgcgga cgcgccagca ccaatgccca gtccatagtc tgtcgtccca tcgctgttaa
     4441 cttgcaaggt caaagcgccg taatagccta ctgttccgga cacgctggca ttttctgcta
     4501 gactgtcgag agcgtcatcg atggtgcagg agataaagca ccgcctcatt tcggcatgat
     4561 cacgctggac gatattttct aagtaccagt cgtcaggatc gagtgggtca tgctttggcg
     4621 tctggaatcc gtagcctagg ccgactccat cgccaacacc gaaatggcca gacagcccac
     4681 ccccattgac cgggccccca tgggctgcgt tctttattgc tcccgcgcca cctccaaacc
     4741 cgggcgaggt gatgaatccg accacggttc gcacgatgcc cagcacctgg cctgcgccgg
     4801 gtatcagcat gtcgagcccg cgtgtgacga gttcgccaat cgcgatctcg gcaatggtac
     4861 tcaggaacgc ggttgctgtc agctcgccaa tggcaaattg gatcgccgca atgcccaacc
     4921 cgatgaagaa ccccccgctc ggatccgtcc ccgccatggg gttgcccatg ccataggtat
     4981 agaggttgag cgactgcttg tagccaacgg ggtccacctg catgaagcgg ccgatgagcg
     5041 gcatgtaggt gcgggcgcgg gcataataga tgccgatctc gcggtcatat tgatggccgg
     5101 tatagccgaa gatggtgggg atggagcccg tggtcacccc atagggcgag tatttcgcct
     5161 ggccggtgat cgcgccgttg gcgttcgcag tggcgatgat ggagccaagc cgatccgcat
     5221 gcaggaaggt tttggcgccc gttgcgcgtt caaccaccag gaagggtgtg gaagcgcccg
     5281 gcgcatagat gaaccgtcgc aacagcgcac tggcatccca caggccggag aattcggcca
     5341 cggtgttttc gctacaagcg cggccaggca aagtgcgaag cggttcgccg tccggccgcg
     5401 cgttctcagc ctgagataca agctgagttg ggtgccggta tggacactca gaaaatggcg
     5461 gagccggagt ccgaaatagg atgattcatc agcatacgtc ctagtttcaa ttcgttcggg
     5521 aatgtagtaa aagactggca ttcgcggtgg gtgccggttt ctgcgtgtac gcagccgttt
     5581 ccctgaatcc gcgcccgata atgggaccag aatgtgggcc cgaaaggtga cggacaatta
     5641 tggcgcgtgc cttgaacaag ctgagtgccc gggcagttgc tacccttcgc acgccgggcc
     5701 gtcatggcga tggcggcggc ctctatctgg ccatctcggc cgatggcacg cggcggcgct
     5761 gggtgtttct gtacacgctg agaggccagc ggcgggaaat gggtctgggt gcagtggcgg
     5821 acgtgtccct cgcagaggcc cgcgagaagg cgatggaagc ccgccggata gtcaaggcag
     5881 gcacggaccc catcgcggcc cgggaggcca ccaaggccgc cacgcgggcg actgaggccg
     5941 ctgagagagc aatcgttacc tttggggcct ttgcagacgc ctatgtggat cggatgagcc
     6001 cggcttggcg gaacgaaacc caccgggagc agtggcgcac gaccttgcgc gttcacgccg
     6061 cagtgctgcg ggacatgccg attgacgggg tggataccga tgccgtcttg cgggtgctgg
     6121 agcctatctg gaaccgtatc cccgaaaccg ctgcccgctt gcgcgggcgg atcgagaatg
     6181 tgcttgatgc agccaaggtg cggggtctgc gcactggcga aaacccggca cggtggcgcg
     6241 ggcacctgcg caacatcctg ccaaagcgcc agcgcttgac gcgtggccac catgcagcca
     6301 tgcccttcgc tcaagtgcca tccttcctga gtgccctgcg tgcccggcag tcaaccgccg
     6361 cacgcgcact ggagttctgc attttgacgg cggcccgtac gggcgaggtc ttgggggcgc
     6421 aatgggatga atttgacctt gatgcggcta tctggacggt tccggccacg cgcatgaagg
     6481 cccagcgcga gcaccgggtg ccgctttgtg cgcgggcggt ggtagtcatc cgcgagatgg
     6541 cgttggccag aatgggcgcg cacgtctttc cgggccatcg cgcgtctggc ctgtccaaca
     6601 tggcgatggc taaagcgctg gaagcaagtg gacaaggtgc cttcaccgtg cacggcttcc
     6661 ggagcagctt ccgagactgg gctgccgagg ccaccagctt tcccagcgac atttgcgaaa
     6721 tggcacttgc gcacacgatt ggcaacaagg tggaagcagc ctatcggcgg ggtgatctct
     6781 tcaatcgccg ccgcgaattg atggaggcat ggggccgcta ttgcgcggat gctgagcgcg
     6841 gcgtgattcg gctggtcgac ttgccgaaag acgcattgcg caataaaatt gcgagctaag
     6901 tctgacgcgt gcccatcatc gggcatcaag agcttgctgg caaaaaccgc atgatccccc
     6961 aaccctgcca attttacggg cgcggggaaa aggctgctcc tcacgcttgc aaatttcagc
     7021 tgctcgcact ctttctgggt cgcgcgtgta ccgccgcgca cacacatgga gaggcagacg
     7081 aatgggcagc accaacgggc gcgaaacata tcgccgcgca aagaagcggc aaaagtcact
     7141 ctcacctgac catatcattg gaacagtggg cgcatactgg gaaggcgaga cccacaactt
     7201 caccaaagag gactttgcgg agctgcatcg gctggctaag cgggcggggt caaaggcgac
     7261 gctggaagaa gtccacgacc gggcaaccca agttgcaggc gaagtgcatg aggtgctgca
     7321 caatctgaca ggtgtcatgc cagcgagcga gcgggcgaag agtgcatcgc tgatccactc
     7381 ggcaattggc cagattgaac gggcgctggg ctactatcaa tgggatgagg atgcgctatg
     7441 gcgaacgaac ggtgcgggcg gttcaatcgc tatgctggag cgattggcaa gccatgcctt
     7501 tgtgcagacc aacaaactgg accgggaaga cgcgcaaaat ttgcgaaaag atgaaaccgc
     7561 aatctcacgg aggctgcggg cgctgcaaat cggagtgccc tataagggcg agttgccacc
     7621 accgcgacat tggcggggaa ttgcgattga cagcgccatc aacactcttc cggtgcgcct
     7681 cagagtcctt cgcctcgcat gtgagagttt cctgcaagcc ccgcctgcaa aacgcccgcg
     7741 cgtcaagaca ctccggctgg gcgatagatt catccgctct gccagtgacg catggcgaga
     7801 attgaccggc aagcggacgc gttacatcaa tctcgaattt gcaatggtgc tcgcccgggg
     7861 aattggaatg tccgtgagca aagatgctct cagaggcctg atttaccggg aagcaaagcg
     7921 gcttgctggg cctgttggca acaaaatccc gatcccggcc atcaccgcag cagcaaaccg
     7981 gcaagtttga ctcaatgccg ctgcgatcaa gaaagccgcc acggtacaaa aggccaaccc
     8041 gcctggagcg gattcggtcg gtacaagaca ggcccgaagg acccgccggg cgactttgga
     8101 atgatgaaaa gcacttgttc aatgaagccg acttcgcaga atttcatgcg attgcgcaag
     8161 gcggagggcg agatatgtcc ctccgacgcg taaggcagat cgtgacccgg gcggcgatca
     8221 atttctacga tgaccggatt ctgggtgccg acaagatgac acctggcgaa aaagtaaggc
     8281 atgtcgaagc gatacgccgc gctgtcagag aacttgaggg ccggattggc atagaggtga
     8341 ttcctgaagg cgagaaagtc gcctctggaa gccgctccca gattctcggt gagctgacca
     8401 tcgaagctca gaaattggag cgttctttgg atttggggtg gttggcccga attgacaaga
     8461 gcacgctcca gatcgcgagg acccttcggt ccatcgccga gcgcctttgc cggaagtcgc
     8521 cactcgacgt ccccggacac aacccgcgca gcggcaagct gtatgacatc atggcattgt
     8581 tgccgacata tctttgcgtg ctgtcacttg cctgtgacgc atggcttctg aacccgcctg
     8641 agaagcagcc aaagcgcgca cttggttcgg tgaataggac tttcctccat gccgcaaatg
     8701 gggcgtacca gcaaatcacg ggcttaagtc tcagccgctc ccgccaatcc attgcttttg
     8761 ctcgcagaat ggccggcggg atgggctttg atattacaga tcctgccctc cgaagccttc
     8821 tgtcccgcaa ccaagccggg acgaccaagg gctaccaagt ccgccgccgt gctcaaactg
     8881 ccaaaaaaac agttccagtt tgagccaaga caaacagcgc ggccatcggc aaccttctgc
     8941 acatcagccg gttcgtcggc gattcaggag agacacgaca tgaccaattc ggacatttca
     9001 gctgcgaagg atcgcgaggc ctactcgatt gcggaagccg caaagttatg ggggatcggc
     9061 cgcacaagcc tttaccgcgc catgtctgaa ggccgtttgc gcaccctgaa aatcggtgcg
     9121 cggcgccttg tacggcgagg cgatttagag gcgttcatgg aagcgcatgc aacgcccact
     9181 ccctaagcca agaaggcgga gcggcatcta tcgcgcctga aaattacagc gaaattcagg
     9241 catcaatttt catggtcgaa ggcgccaatg gctggcagac acaatgcaac cggccgcagc
     9301 acaggcggca tgaccgcgcg cgaccggaaa atccatggtc cgccaaaagg cagcccctgg
     9361 gtttggctcc ccacagaact gctgaatcat ctggcctggc gcagcctttc gctcaatgcc
     9421 cggaagatca tcgacttcct cttgatcgag cacagacacc acggaggccg ggagaatggc
     9481 aatctcgccg caccttacaa ccagcttgag agatttgggg tcagccgccg gctcatcagg
     9541 gcgagcttcc ttgagcttga ggccgccgga cttgtgcgga tgacaagaca aggcggcaaa
     9601 tacgccggcg gacgtgagac ctcaacctat cgcctcacat tttatgccgc gcatgacaac
     9661 gtctcgccaa ccagcgagtg gcggcgatcc gatggggcca aagtgaaggc ggcaagaaag
     9721 gacgcggccg agaacaagag ccgggcatcc gaaaaccggc gcgggcggcg cgccaatccc
     9781 gatttggagt gcaccatggt gaaccttgag agtgcacctt cgtgaacatt gaagccgttg
     9841 tacgccgaac ccggcgttcg ccaccatccg cccgccgccg cctgaaaagt gcaccatcgt
     9901 gaacagcagt atatactctg gtcctatcac ttgcgtgacc agagcgtctc caaactggag
     9961 taggagatgc agtgatctct tgtcagtagc gtctgcgatt gatcgactgc ccgctcaacc
    10021 gcatactcag tttagccaac ttgccgagag cgcttccgct tgccgaagaa ccgtttgcac
    10081 cgctgcgtcc tgaagatcgg gcgggtagcc gtgcttgcgc agaatccgct tcaccagcac
    10141 gcgcattcgc gccctggcac tttcccggtg tgaccaatcc actgaaacgc tgcccttgag
    10201 gctgacgagg agttcgtgtg cgatcacctt aagggaatcg ttgcccatca gttcgacggc
    10261 gctctcgctt tcggccaggg catcatagaa agcgatttcg tcctgcgaca ggccttcatc
    10321 ttcaccgcgc ttgcgggcgg cgcggacttc ctttgcgagg tcgatgagtt cctgcagcac
    10381 ctcgacggtg ctgatcgcgt tcgtgtggta gcgggcaatg gcggcttcga ggcgctcgga
    10441 aaagcgcttg gtctcgacca cattcgttct gctgcgggaa cggatttcat cgttcaacag
    10501 cttccgcaac gcctcaagcg cgaggttttt cttttcgagc tgttgaacct cgacgaggaa
    10561 ctcgtccgac aggattgata tatccggcgt cgtgatgccc gcggcagcga gaatgtccaa
    10621 gatctcggtt gaaacgactg cccgatccag gatctgctgg attgcgaact cccgatccgc
    10681 actgctcttt cctgagcctt ctgccgattt cacgagggcg gcacgaaccg tctggaagaa
    10741 tcctacttca tcgcgaatgt cgcgggcctc atcgcttgcg gatgccaggg cgaatgcctt
    10801 ggaaagcgcc agcactgcat ccaggtatcg acgattttcg cggcgcttgc cgtcctcgct
    10861 ggtttccttg gccgcggcgt cgtgctggcg accaagtatc cattcgatcg cttcggcgag
    10921 cacgaccagg cgctgatgcg gcgtaccagc caagcctcgc gcataatcga agccgtggta
    10981 cattgccgac acgacctcgt acttctcaag cagcacggca accgcttcgg ccttatcgat
    11041 ccccgcctgc cgctgatcgg aaccggaata ctggccgagc gcgttcttga ggttctgggc
    11101 gatgccgatg tagtcgacga cgaggccggc aggtttgtcg cggaagaccc ggttcacccg
    11161 ggcgatggcc tgcatcagcc cgtggccctt catgggcttg tcgacataca tcgtatgcat
    11221 cgacggcgcg tcgaagccgg tcagccacat gtcacggacg atcacgagct tcagcgcatc
    11281 gttcgggtct ttggcgcgct tggccaagag atcgcgcctg gccttggttc cgatatgcgg
    11341 ttgccaactc ggcggatcgg cagccgaacc ggtcatcaca accttgactt cacccgctgc
    11401 gtcgtcgtcg ttgtgccaat cgggccgaag cgcgatgatc aggtcataga gcgccacgca
    11461 gatacgacgg ctcatgcaga ccaccatcgc cttgccgtcc atcgcggcaa cccggtcctc
    11521 gaagtgccgc accagatcgg cggcaaccat cgccagccgc ttccgagatc cgaccaacgc
    11581 ctctatggtc gcccacttgc gcttgatctt ctcctgttcg gtgaccgcct cgtcttcggt
    11641 caactccgca atctcggcat cgatctttgg cttctcctcg tcgggaagct cgatgcgcgc
    11701 aaggcggctc tcgtagtaga tcgggaccgt ggcgccatct tccaccgccc ggctgatgtc
    11761 gtaaacatcg atgtattcgc caaacactgc cggggtattc acgtcgtcct gttcgatcgg
    11821 cgttccggtg aaccctatga aggaggcatt tggaagggca tcgcgcagat actttgcaaa
    11881 gccgtaggcg atctcgccgg tcttctgttc gatccgcgcc ttgaaaccat attggcttcg
    11941 atgggcttcg tcggcgatca cgacaatgtt ccggcgatcc gacaacatgg gataggtcgc
    12001 ctcgccggct gccggcgaga acttctgaat ggtggtgaaa atcactccgc cggaggcacg
    12061 gctgagtgtc gattgcagat cctcccggct gtccgcctga atgggcgttt gccggatcag
    12121 atcgcggcac atcgaaaacg tgccgaacag ctgatcgtcg agatcgttgc ggtccgtgat
    12181 cacgatgatc gtcgggttct ccatcgccgg gtgccgtacc agctggccgg cgtagaacgc
    12241 catcagcagg ctcttgccag acccttgcgt atgccagatc accccgatgc gcttatcccc
    12301 tcggggatag tccgtgacgc cgggaagccc ataggctgcc ggctcctcat gaaagcctgc
    12361 catacgatgc gcgctcgcgg acgtaccggc attcatggca agggcgcgca cggttgattc
    12421 cacggcgcga cgcacggcat ggaactggtg ataccccgcg atgatcttcg caaggccggt
    12481 gcccgtttcg ccgaacaccg tgaaatcgcg cagcagatcg agcaggcggc ggcgatcaaa
    12541 aatcccttca atcaggacgt cgagttccgg ctggcccttg gcggcaatga ccttgccgtc
    12601 cgtggtgcgc cagggcatga agcgctcccg atcggcggtc agcgagccga cgcgtgccgt
    12661 catgccgtcc gagatcacaa gaacggcgtt cgtccggaag agcgagggga tttgcgcctt
    12721 gtaggtctga agctggttgt gggcgccggc gagcgtcgcg gtctcgccgc ccggcgcttt
    12781 cagttcgatc acgccaagcg gcagcccgtt gatgaaaaca acgatatccg gccgccggtt
    12841 gacgctgccc tcaatcaccg tgaactggcc ggtcgcctgc cagtcgttta cggcaagatc
    12901 gtcgaaatcg atcaggcgga ccttgtcacc ccggatcgtc ccgtcatcgc tatagaactc
    12961 gacatcgacg ccctcaacca tcagcttgtg caagcgacgg ttctcttcga cgagcgacgg
    13021 catttcggtg gcaagcacct tccgtagcgc atcactacgc gcctcagccg ggatcgcggg
    13081 gttcagcctt tcgatggccg ctgtcaggcg cttgaggaga acgacatcgg cataggcctc
    13141 tcgctcgggc gccttgccat ccgggccgat ctcggcgtcg gtcgcgagtg cgtagccgag
    13201 gccaccgaga tgatcgagga ccaccttttc aatggctgct tctgaaagat atgccatcag
    13261 gcgatcctcc caatggttcc gagtttgacg aagacggcaa tccgcctaat catgaaagcc
    13321 tccttccatg agcgagataa gcagatcttc gatcgcctgg tcatggtctt cgggcggata
    13381 ccaaatcgga tgaatgtccg cctggccaag ctcgttccgc ctggcttcac ggtccatacc
    13441 ttcgctcagc ggtaagaaag cgtagtggcg aggcgtctct acgacggcgt cctgttttat
    13501 ttcacggagc gcataaaccg ttcgatccac cgacagacta catccgagaa agagcaagct
    13561 tgtattggca atcagcagct tgaaaagctc tcgatagttg ccgccgtctc catagcaggc
    13621 tgtgtattcc ccgccggtga gaacgcggcc ttgagcgcta tctgcttcac catgaagccg
    13681 gagaaggcaa tgaggttcat ccgcaagcct tcgcggtgcc tcattcagtc gagtcccaga
    13741 gaactcgccc ttcaggcggc aatcggaagc ttcatagacg cggttcagga catagtcgaa
    13801 gttcgttgtc aggcaaccac gtttgaagat cagcggaagc agttgtaccg gtcccctcaa
    13861 ggccttcacg cgtgagccga acgcattttg aaccgcctcg gcaaagacat ctggtcccat
    13921 acgatcaagc accagctgag ccgcttcttc gtatcgatat gccgccaggt gggcgcgaac
    13981 gtcgtcggca atagcgggat agtctgcggt caatcctgct agaaacgcgc cccataaagg
    14041 aaagcctgtc ggccgcgaaa ggcccgcccc gacaaacggg ataacgcttt gtcgtttgag
    14101 ctgcgtgtgc agccggttga accgatcaag gcaattgaac cgctcaagga actcgcgcgc
    14161 gcgatccatg gcgttctgtt tctggcttgc aacccagtcc agaaaatgcg cctcgtagtc
    14221 gagcggcgcc atcgtcttaa ggacatcggc gggaggccat tcaaaggtcc ggacggtgac
    14281 taggccctca tcgttgagct cttcgacgaa gcgctgacgg aaatactcca cgccgcacct
    14341 ccgttaagcc gataaggccg cgtcaccgac gcgaacttcg cccgacatga gcttcggaag
    14401 caggaactcg cgcgtggtgg cgagggtgcg gttttccttg tcgttggcga cgatcctatc
    14461 aaacaggggc ttggccgtct cgtcgaatga ccgaaggagt tcgagtgccg ggacggtcac
    14521 ttcgatcggt cggaagtttg ctttgctgat ttcctgaaag gtcgatccat tggcgttctg
    14581 atggaccgtc tccatgttgg cttgcgtcca ttgccagacg aagacgttgg acaagcgctt
    14641 gttgcagatc atcgcgatga agccctgatt gaccgcaacc ggaatctggg cgatcgccat
    14701 gtagccgatc ggggcacgcg atgagagaag caccgttccg gcaggcagca agccagagcc
    14761 aatctgggac aggccagctt cggtgatctg ccgttccgtg ctgagcagca ccggagtgct
    14821 caacgacgag aggtctttcg gagtcgccca agcgacgtcg ccgccccaga aggaaggctc
    14881 tttcgtgctc ggcgttgagc cgccgagaac ttcgacttct tggccgatcg tggaagtgat
    14941 ccacccctcc ggcttgcctt catcgtcgag gcggtcgggg aaaagggacc agatgtccgg
    15001 ggcgaggtag ggcgcgcggc cctcaatctt cgcgcgggtc gggccgaagt cgacgaacca
    15061 atccttgaag atcgcccgcg ccatcgcctc cagcgtctcg ttcatccgcc ggttcagctc
    15121 gatcttgtca tcaagcgcac cgagcacgga ggcgatagct tcttgcacga gtggggacgc
    15181 ccatgcgatg cggtgtccta tcagatcacg gcgagttatc tgaggctgtg ccgaaccagt
    15241 aacaaccgaa tacggcgggg aattaagtaa cgaataaaac aaaaagcggt tgctgcctgc
    15301 ccgtggcaga atagccatgg cgttgttcga aacccaaagc ggaccttttg cccacaaaac
    15361 cataccgctg tttgaaccgc ggcatgtcac gagaggtacg ccctcagggt acattggcgc
    15421 agaagtccag ccaatgatcc cattcgcccc gtgaaccgga ataggaaaat cggcagacgc
    15481 ttgctccgcc atctcctctt ttgagagata tcgaccttgt tgaatctcaa caatttcaga
    15541 aagtgccgtc ggaacgatct caggcatcgg gagcaacgcc ttttagtcgt gcacggatga
    15601 tgccagtgag ccgttcggac tcggcgaact gcagctcaag ttttcctttt agcccagtta
    15661 accgctcggc aaacggcacg tcgtcgtctt cgatatcggc cgtgccgaca tagcgccccg
    15721 gggtcaacac gtatccgtgg gccttgatct cctccaaggt caccgccttg caaaagcccg
    15781 gcacgtcctc gtaagcgccc gcttcgcctt cgccgcgcca ggcgtgatag gcgcgggtga
    15841 tcttctcgat atcgccgtcg gaaaactcct tacgcgtgcg gtccaccatg tgtccgagct
    15901 tgcgcgcatc gataaacagt atctcgccgc acctgtcgcg cagctttgcg ttgcgggcga
    15961 tgccgttcga tttgtcccgg gcaaggaacc agaggcagac cggaatctgg gtcgaataga
    16021 acagctggcc cggcagcgcg accatgcaat cgaccacacc cggcgcgccg ttgacgccct
    16081 ctatcagcgc ccggcgaatc tcgccctcgc tgttctgggt tgatgacatc gagccgttcg
    16141 ccagcaccac gcccgccacg cccgagggtg agaggtggtg cacgatatgc tgcacccagg
    16201 cgaagttggc gttgccagcc ggcggcacgc catatcgcca gcgcacatct tcgcgcagcc
    16261 ggtcgccgcc ccaatcagaa atattgaatg gcgggttggc gaggatgaaa tcggccttga
    16321 ggtcgcgcag ttcgtcctta tggaagctgc cctcgctgtt ccacttgata tccgcgtcga
    16381 tgccgcgcac ggcgaggttc atcttgcaaa gccgccacgt cgtgtaattc gactcctgcc
    16441 catagacggc gatgtcgccg atgcgcccgc cgtgcagttc gacgaacttc tccgactgaa
    16501 cgaacatgcc gcccgagcca cagcagggat catagacccg gcccttgtag ggctcgatca
    16561 tctcaaccat cacccgcacg actgagcgcg gcgtatagaa ctcaccgccg cgcttgcctt
    16621 cagatccggc gaactggccg aggaagtatt catagacgcg gccgagcacg tcgcgcgcct
    16681 cgcccttttc ctggccgagc gagatgccgg agatcaggtc gatcagttcg ccgagcatca
    16741 ccgcgttgag cgcgggccgg gcgtagtctt tcggcagcac ccctttcagg gtttcgttgc
    16801 gcttctcaat ggcgatcatc gcttcgtcaa tcatctttcc gatcgacggc tgctttgcgt
    16861 tcgcctgaag atgcgaccag cgcgcttcct tcggcaccca gaagacgttc tctgcggaat
    16921 actcgtccgg gtcctcggcg ccgtcaggat aattggccag cagctcggcg cgcttggctt
    16981 cgaagctatc ggaaatgtgc ttgaggaaga tcaggccgag cgcgacgtgc ttatagtccg
    17041 acggctccat gttgccgcgc agcttgtcgg ccgccttgaa gagatcggcc tcgaagccaa
    17101 gatcgccgcc cttgccgttg gcgcgggcag gttttgctcg tgtagcaacc ggcaccgctt
    17161 cctcgacttc ggcttctacc gcgcctctga gagagaccga gccgccgcga ccacgcccag
    17221 gcgtaacaat gccctcggcg acgagttcat ccttcaccgc attgtaggcg gcttcatccc
    17281 actgcagggc ttcgcgcaaa cgtccgttgc cggcggagcc gccaagctct ttcagcaccg
    17341 tcacgaacct gtctttctgc gcttgttcag ccattccatc cctcgataag cctatcgctg
    17401 cgtactatca ttgcggggat tatccgggtc cgattgtccc gcgactatcc ccgatttgtg
    17461 gaaaacctca agaagcggtg tcgggccaag atgggaactg tcgtgtgtga ccagccagag
    17521 ccacctcgct ctcacccgca ttattgagaa tggagaccag gtggcctgat tcattcgagg
    17581 acagtgtaaa cttggctcgg cattttgtcg ccgcaaagag ttttctcgcg tttcgccttc
    17641 caagcctttg cgcgatgttt cagcttgctg tggatactgg gctcgaacca tcctatcgct
    17701 gcgccactac gcggaaccga ggttttccac aatccgactc gcgcatcgac aatcttgaga
    17761 aacaggatgc tgaacgccca gaactcttcg ggagaattgg tggcgcacat cttttcgaac
    17821 cagtgccgcg cccagcggtt tcgatcatat gcgaagcgcg ccgccttcgc cgcgttaccg
    17881 atcagacctt tggcgtccgc atatcggtcc agcaacgctt cggatgtggt tgattccatg
    17941 ccgaagccga tcaccatgag agcccgtgca ttggcagcag gttcagggcg ttccaaattc
    18001 ttgcgcgcgt actgttcaag gaacgctgcc tccccggcca ttagggccgc cagcacctct
    18061 tgcgccaact gatggtcatt cgccgcgcga tccagccgct gcgcccgcag ctcatccagc
    18121 gcgccgccat cggcactctc ccagatgcag atggcctcaa gcgacacgcc cgagaggccg
    18181 gacaccaggc tgacgaaagc ccgcctgcct gagaggcggt cgaacagttt gcgggcgagc
    18241 gtcaggtcgt ggcgggaaac cgaccttgcc agcatcaagg cgaagttctg gacgtgcatg
    18301 agcttgcgat cgtccaggcc aaggagctgt ccgaccaatg cggcgcaatg atccggcgat
    18361 gtcgaaacgc acgcatcgac cgcttcaaag cccacgtcct ccaggatgag ccgcgccttt
    18421 tcattcgtta gcgccgtctc aaaccgattg tacgcctccc acccctggcg ctggcgcgct
    18481 tcaaagtctt ccggcgtttc agacaggcgc ttgaagaatg cctcggggcc taagacttca
    18541 tcgggctccg ccaacgacag gagcggcggg atcggacggc ctgccatctc catgtcctgc
    18601 tcaacagaag gcggcaagaa cggcagttca atatcaatcg cctttgcggc tgctgcggtg
    18661 aggcgcgcgg caattgccgc gtggcactcc gctccgagca cctccgccgc gacgctgcag
    18721 aggcgcgggg tagtcctgtc caacgcctca tcggcgctga tcatcccttg ctcggccgca
    18781 ttgatgacaa tgagcgagcc ataccaaatc tcgaagtggt tcttccgagg atcgagcaag
    18841 gcggcgctcc atccggtagc tatcaccctt tcgatcagat cgcggtcttt caagtgcgcg
    18901 ataatgtcca ttgcttccgc gcgcactgac gatcgctcat tctccgcgaa ctgcccgacg
    18961 agagtccggc tgcgcggggt cagaatcgtg ccggagcacc gcgcgaacaa cagcgcgaca
    19021 agtgccctgt tgtgctcggc cgactgcatt gcttgattca gcgaccattc cagcttctcg
    19081 ggcatcgccg gcttcagcac ttcggccagc ttgagcaagg gtgggccgtg agacggcaat
    19141 gcggccaggt ggtcgagctg ccgatcgcca cccaggtggg ggaatgcgat ctggatggca
    19201 tattgcgaag cgatccagtt atcgtgggag tccttggatt ctggctcgcg cggcgaacta
    19261 agcgtatttg cgatttcaac gagcttgcca accgtctcgg aatccagcgc tgcgcaatgc
    19321 ggctccagca aagtgatacc tcgcatgagc tccgctgatt cgcgggcgac gattgatcgt
    19381 gcgattttgc gctgggcctc gatcgccact tccggcgcga accggaccag ccccggaagc
    19441 gcgtccctca caaagtgatc ctcctcgccc atagaggcgt ggcgtgccac ttcttcagca
    19501 tcgacttgcg agtatttatc ggcggtcaca tcgagattgt ccggctttgt ggatgcagga
    19561 tcacaaggat cggtggcaca gtatttttcg acgagcctcc atcccccgaa tttctcccga
    19621 tccttggtaa gcgcctcgat gagctgctct gcgcgcccgg cgtcctcgac ggtgctgatg
    19681 ccccgcaaga tcgccaccag cgcccattgg cccgtctgcg atgtatcctt ctgcgccagg
    19741 aaatccgacg cctcaaggag cagcttccgc gtctcctgcc agtcacggcg gttgtaacgg
    19801 atcagcgcga gatactcgtc gtatggcgcc cggaagcttg aattgagggc acgcgaaaaa
    19861 ctgcacgcga caagctgctc ggcaaagccc gccagcggca tcccggccag catctcgaag
    19921 gcatcctgat ggagcagcga aggatcgtaa cccttgcact gaagcatgtt cctttcgagg
    19981 aacactcgtt ccgccgcgga cagctccgcc attttccgcg cgagcgcttg gatctcctcc
    20041 tccgtgtcct tggcgacctt atcatcgctg tcgtggccgg gggtgctcat cacgccgatt
    20101 ttcggatcga gggaatagga gcggagccag ttcgacacat gcttgctgat gatcgcccag
    20161 cattccggtt tgtgtcggca cttacgcagg gccgccgaaa gccagtcctt gttggctgca
    20221 tagcgggagt tcgccgccga gagatcgaac agcgccaaca tcgccgcatc cgtcgcgttg
    20281 cgcgcgacgg cgacaaacgc cgggtagttc tgcgcgtcga cattctggag acgcaggaaa
    20341 ccgcaaatga gagcgcgcct gatcgccagt gagcattgct catcgatgct ggacaccatg
    20401 acgccggaga aaaccgcatc ggcggtcttg tcgagggcag cgatcggctc aatcagctcg
    20461 tccagcgcat cggccacatc gcgcccgttg cgctcggcct tctgcagcgc cttgatgatc
    20521 gagaggccga gcgccagcga aagcccgcca tcggcaagag catatagagt ggaatcctcc
    20581 gggagcggcc ggaagaaatg ctctgctgtg acggcgagaa ggtcggcgct cagggcgtgt
    20641 ccgcctttgc cgtcggcgcg gtcgaagatc aaccggtctt cgcgctgttg ctgcttcact
    20701 cggtcgatta tgtcctgggc gtgttgcgcc agccgctttg agaattgatc cggcggctcc
    20761 gcagcgttcc cgtcgtgcgc gccgacgcgg atatgctcga agagcagtct ttccacgctc
    20821 agctcgtcga agttcttaac ctcgaccttg tccagcaggt cgaaagcgat ccccagaatg
    20881 cgtgggttgc ggagccgcgt gagcaccgcc ggtttaatct tgctcgcgtc gatgcctttg
    20941 gccacgagaa tctctttcag ttcccgctcc gtccattccg gtacgcgtac ggtggcgatc
    21001 ttggaatgca ccgcagagcg tatgcgctcg tcaaaatagc tctcgcgcga agttatggtg
    21061 agagtaccgc ctagctgctc aacgaccatg gcggcgtcat caagccatcg cggccagtca
    21121 aatccaggct gctggttgag accgtcgaca cacaagatga agcggggcga tgcctcgttt
    21181 gcctgctcct tccatcgcgc gagccgcctt tcccagcgtt tgcgggggat ttccgtgtcg
    21241 ctgtcgtcag tctgctggat cagcttcgag gtcagaaagg gcgtgaggct cccgtacgcc
    21301 gcaaccggct tgaggtcggt cgccgggatc acgaccagca gcgggcggac gccaagttca
    21361 agccagctct gcgcaagcag ccatgatttt ccgcttcctt ctcctccgac gagcgccacg
    21421 atcttgcgcg agggggccgc cgacagatgc tgatgaactt gctccacgat ggcggaacgt
    21481 tgccgaagcg agagcgctcc ttccgcgttc ggtgcgagaa cctggccgaa ggctgccttg
    21541 gcgcgccgcc gatccgcgaa aacagcctca agccactttg cattggcgct cctcgcattg
    21601 gcctgtccaa gcgaggattc ctgaagcttc tggacaagag catcagcgct tgcgcgaaac
    21661 gtttccgctc tctggattgc ggcgagcgcc gcttccgccc tctcaacgca ttcaacttca
    21721 ttgaggtgtt tcctaagaaa cacggaggtt tcttcgctcg cgagagcgca agcggcggcc
    21781 agcggcggaa tggcggaagc gcttggccag tccagcacaa gtatgccaat gccgttcttg
    21841 gcggcggcgg tatgcattgg gtcgagcaat tgcgtgcttg cctcgaccgt ggcgccgaaa
    21901 acccacacgt caggcggcgc gccgctgccg atcaaccgcg taatctttgt cagaacctcg
    21961 ttatcgttga tctttttatt gtaaagcttg ccctcaaagg agatgtgatt gctcgcagta
    22021 gaggatgtcg cgccgtcttt cccattctgc aggccggatt tggccaaacg aaaatcctgc
    22081 ccggacgctt tactcaaaac gcacgcgaga aggccctcaa aacccgctgg tcccgcaggt
    22141 tcaaggctga gcaacgcatc gcgcaggtgg cccacgtgct tcgaaagaag atggttgcgg
    22201 gcttccgtcc cctttgctga accgtccgtg tcaggagcgt cgctcttgtt gctggtcatt
    22261 aggcatctca acctgaatct attgacggaa gcggaagtct cactggagta gacaaacttc
    22321 ggcgcggtat ttcgagcgtg agccgcgccg cgtggcccaa cttgtcgagg aaacaaatcg
    22381 ataaaggagt tggccgacgt caatccatgc aacaatgacc gggcaacgat tgcgtggtga
    22441 gcagcgataa ggcagtttgg cagttcaagc tattgggttg atcggcatgt ggcatgtatg
    22501 cgccaatggg tccgctttga ccaatcgcgg ttcattcaat ctcgcgtcac tcggtcggct
    22561 ttcggattga ataaaaaact tgttccggca caagtaggaa aggggaacgt gtgcgccgcc
    22621 gtgccaactt atgcggaact attcggggcc accgattccg attattgcgg gaaatcgaaa
    22681 atagaaatag gggccggaag tggggccagg cgcgtgaaaa agcgaaaaac ttagtcaaat
    22741 caatggtaga tggcggaggg gatgggtctg atatccaacc ttctctggcg cggctctact
    22801 ccgaattgca caggttccgc aagctggctc gtgcgtagat ccggcagtcg tcccttcacc
    22861 gattgcccgc ggcaatccta atctcgactt attgcgctga caggaatagc ttgaccttat
    22921 gagcaagctc gtccgcatat tcgcgttcgt ctttctcgca gccttcgctg cgggcacggc
    22981 ggcgaatgcg gcggctgcta cccaaatgtc gctgatgatg tccatggcaa atatggacga
    23041 cggcggtatg gcggactgtc aggactgtcc cggcgatgac gctcagacgt cggcatgcga
    23101 ccagttctgc gtgaccaccc tagccgcgat ctgcacgcct gcagggacag tcctgccgaa
    23161 tgtcacggat gtcgtggtga tcggtaccgc ggcatcgccc ggtggtcgtg tcgggccgcc
    23221 cgatccctat cctccccgaa ccatacacat gggctgaagc ggccgcggca agatcgctcg
    23281 cagccgaccg tcgacgcgcc ctggctacgc tccgcgcgtc acatcaggct attccacgga
    23341 cggttgatcc atgtcctatc cacgtcttcc ggcgctgacg cgtcgagcct tcctcgcatc
    23401 agccgccgtt gtctcggcga gcacgattct tcgatcgcaa gcccgcgcga cgagcgttcg
    23461 ggcgttccac ttgcgcgccg gatacggtcg ggcccagatc gcacccgaac cctacggcca
    23521 gacacccgtc tggtgctatg acgggagcct gcctggcccg gagatccgcg tccgtcaggg
    23581 agaacgcctg cggatcgcgg tcgcgaacgc gttagacgag gaaacgacag tgcattggca
    23641 cggcgtgcgc gtcccgaacg ccatggacgg cgtgcctcac ctcactcagg cgccgatcgc
    23701 gcccggcgaa gtattcgttt atgagttcga cgcagtcgat gcgggcacct tctggtatca
    23761 cccgcaccat cgcagctttg agcaggtcgg acgcggtctc tacggcccaa tgatcgtgga
    23821 ggaagccgtt ccggtccaag tcgaccgcga tctcacgtgg ctgctgtccg actggcggct
    23881 gaccagatcg gcggaggtgc gggatgattt cggcaaccgc cacgacatga tgcacaacgg
    23941 ccgggtgggc aacacggtca cgatcaacgg ccgcgtgcca gatacctttc ctgtgcgcaa
    24001 gggagagcgc attcgcctgc gcctgatcaa cgccgccaac gcacggatct tcggtctcga
    24061 tttcgggagc catgagcccg tcgtcattgc gctcgacgga cagccggtcg agccgcatgc
    24121 gccggaaggt cggctggtgg tcctcggacc cgccatgcgc gctgacgtca tcctcgacat
    24181 gaccgctgat gcaggaagcc aggtttccgt ggtggatcgt ttctacgagg ggctggagta
    24241 ccgtctggtt gatcttgcct acgagaagca gccgctgagg gacacggcac cagactggcc
    24301 gctcgccctc ccgccaaacc cgctgccgga gccagagctc gcgacggccg cccgtcacga
    24361 ggtcaccttc aacggcggca tgatgggagc catggtcatg caggagatgg gcggcagcat
    24421 gggagaggcc gcgccgggag gcatgatggg cgggatgggc tcgatgatga acatgatgca
    24481 ttccggcggc atctggttcg tcaacggcgt ggcggcggaa ggtcacatca tggacccgat
    24541 actgacgctg gcgcgggata aaagccatgt catcgcgatg acgaacgcga cggcctggca
    24601 tcacccgatc cacctgcacg gccattcctt ccgggtgatc tcccggaacg gcaagccgac
    24661 ccgccaccgc gaatggcagg acacggtgct catgtcacct cgagagcgcg tcgagatcgc
    24721 tttcgtcgcc gacaatgtgg gcgactggat gctccactgc cacgttctcg aacatcaggc
    24781 cgcaggaatg atgggtgtct tccgcgtcgc ctgaccaatt atctcgaaag gagaaccgaa
    24841 atgcgaacaa tactgagcgc cgtcgctctt gcctccttcg tctggggcac tgccccggca
    24901 ttggctggtg agaaggatgt caccctttac aagaacccgc agtgtggctg ctgcgagggc
    24961 tatgccgact acctgcggga caacggcttc accgtcacca ccaagcccac gcacgatctg
    25021 gccgaaatga gccggcaggc gggcatcccc gacgagttcc aaggctgtca cctttcattc
    25081 atcgatggct acgtcgtcag cggccacgtc cccatagaca cggtcgagaa actcctgacc
    25141 gagcggcccg acatcgccgg cgtgacgcta cccggcatgc cgctgggctc gcccggcatg
    25201 agcggcgtga aggaggcacc cttcgagatg ctggagatca caacgtccgg cactatcggc
    25261 ggagtctacg cgcgcgaata gcaggcctga caatcttgac cggacgaacc cgatgatgga
    25321 cggcggaatg atggacggca tgagcggcat gatgctgggt atgggccttg gatggctcct
    25381 gctcatcgtc atactcgttc tctcagccgc ggcgctggtg aagtacctct tcttcagcgg
    25441 caggaaatga gaagtacccc gatcctggcg gtgatggcca tgcttgcggg agccgctgtg
    25501 atggcgtggg tctggctgtt cggagcgggc tcgggcgagg ccgcggggat tgcgcaggga
    25561 cggcaactct atgccgaaca ctgtgcggtc tgccatggcg ccaacttgga aggtcaatcg
    25621 gactggaaga ggccgcttcc ttcggggcgc atgcccgccc cgcctcacga cgcctccggg
    25681 cacacctggc atcatcccga tggggtcctc ttccggatca ccaaggaggg tcccgcagcc
    25741 gtggttggag acggatacga aagcgacatg ccgggattcg ggaacatgat gagtgacgag
    25801 gagatccgtg ccgtcctggc cttcgtcaag agcacctggc cggagcggga gcgcgcttat
    25861 cagacggaga tgagccggcg cgagaaggac gaggcgcgtt gaaggtaatg cctgtcgacg
    25921 cttgcgtcgg cgtcttggcg gccgttttgc tgccggccat cgcgttgcac gagcccacca
    25981 gagcccgacg tcgccgagga cagagccgcg cgatcgctag aggactggcg cggcaagctc
    26041 gtgttttgct caaggtctgg agtgatctgg tgcgcgactt gcagcgcgag aatgccaacg
    26101 ctggggcgaa ctgcctctcg acaatccgat acggtcagat ccagcgtcgc acctcccttc
    26161 tgtacgtctg atattcggca ccgaggtcgc cctccaggac ccgttcctcg atccggacct
    26221 gacggtgcac agccgcaagc agcaggaagc tcagggtgaa ttggatgagg ttaggcagcg
    26281 cgagagccaa cccgaggaac agcatcacct gaccgacgaa cacagggttg cgcgagaagg
    26341 cgaacggtcc tgttcgtacg atggtgccga gctgccccga cgctgccccg atcctccagg
    26401 atcggcccat atggatctga gaatagagcg cgaggcctgc tccggccgag gcgagggcgg
    26461 ctccgaccag atcgccgaag cggccgtcga gtgcgttgtg taccggggcg gccagtgggt
    26521 cgcctaacac cagcgccgtc agcgcgtaga aagcgacgcc gacgaaggag agtcggaaca
    26581 ggagcgctgg gagcgcctgg ctctccgatc ccttgccgaa cagccaaaat gattggcccg
    26641 acgcccgtgc cgcacgggcc gtcagaatta ggaatagcgc catatagacc agcactattg
    26701 cagagcagac ggtctcgatc ggtgacatgt tggtcttcag gccagccgat cagggatagc
    26761 tcgggtaaat ttcggtcgat ccgtcgcggc gcaccagaag cacgtcatag gatcggcatc
    26821 catcatgagc gaggcggcaa cggcgagtcc ggttgtgcga acaaaggaca cggcatcttc
    26881 tcctttgttc atgcggccgt gtccgccaag cggcggagct tccgaagctg ggtttctctc
    26941 ggctcgtgca aatcgagcgt gcccatgaac tcgccctcgg catccatcag gtagacgcca
    27001 gcggtgtggt ccatcgtgta gttgccgcct tccagcggca ctttctcgta atacgcggcg
    27061 aacgccttcg cggcctgatc cgtctgttcc ttcgtgccgc gcagggcgag aatgcgggtg
    27121 tcgaacgagg tcatgtagct tgccagcagt tcctgcgtat cgcgctcggg atcgacggtg
    27181 atgaacagcg gcttgatctt gtcggcatcc ggcccgaggt ccgccagcag gccgctcagc
    27241 tcgaagagcg tcgtcgggca aatgtcgggg caatgcgtga agccgaagaa gacgagatag
    27301 ggctttccct ccaggtcctc gtcggtcagc gtctcgcccc catgcgttgt cagctcgaac
    27361 ggaccgccga tcgcggcaac gcccgtgacc gtgctcgaag tgagcgctcc aggcttctcg
    27421 acgcccggca ggatgccact gaaggccaac agcacacccc cgacgatcgc caccgcgccc
    27481 caggccgtga tgcggatgat cttcaacgct ttcattgtga tacgggcgct ccgtggcctt
    27541 cgtggttctg aggcgcttcc ggcgttgcgc ccatcggcac aatcgcgaac tccaccgcga
    27601 catcgccggc cttctcgaag gtaagcgttg ccggaaagcg ctcgccgtcc ttcagcggct
    27661 ttgccggatc gacgaacatg atgtgggcgg cgccgggctc cagcctgagg gtttcgccgg
    27721 gcgcgattgc aattccgtct tcggcgggac gcatccgcgc cacgccgtca gttattgtcg
    27781 actcgtgaat ctccacggcc tcggcggcat ccgaggagcc gccgagaagg cggtcgggct
    27841 cggtgccggt gttggtaatg gtgagatagc cgccagcgac tggtgctccg ggcggcgtcg
    27901 cccgcgccca gggatggtca atcgcgagtg gcccggcctc gtagtcatga gcaagcacac
    27961 cctgcgttgc cgcgctcagt accaggccga gcgcgagggt cttcaccagt ttcatcctgt
    28021 cactccgatt ctttcgtttt tggctgcgct cgcacccctg ccgctcgtcg aagcgcgatt
    28081 ggcacggaag cgaaggagcc gcagcgcatt gatcgtgacg agcacggtgg cgccagtatc
    28141 ggccaggatc gccatccaga gcgtcgtcgt gccggtgagc gtcgtaacca ggaaaacggc
    28201 cttgaggccg agcgcgagag cgatgttctg ccagatattg cccagcgtcg cacgcgacag
    28261 ctccaccaac tcggccacac ccgtaacccg gttcttcaga agcgccgcgt cggctgtttc
    28321 caacgcgacg tcggtgccac cgcccatggc gatgcccacc gaggcagctg ccagcgccgg
    28381 cgcatcgtta atgccgtccc ccaccatagc aacgccacca tgcgctctca gcgcgccgat
    28441 ctcggagagc ttggcgtcgg gcagcagttc cgttttggct tccattccca gaccgccagc
    28501 gattgcagca gccgtgcgcg cgttgtcgcc ggtcagcatg acagcccgga cgccgagctc
    28561 gttaaggcgt gccacgccgg cggcggcatc ctcgcgcggc tcgtcgcgca gggcgatgat
    28621 gccgaggatc ctcttgccgc tcagcagcac aacggcggtc ttgccttcga actccagcga
    28681 tctcaggcgg gcaccgatct cttcgggtac ctcggcttgc tcagccgcgt agcgcggtga
    28741 gccaaccgaa acgaagccct cccgaaggcg tgccgttacg gccttgccgg ggaccgccat
    28801 cgccccgccg aacgtcttcg gcagctccag gccgcgggcc tccgccgcct cgacgatggc
    28861 ggcgccgagc gggtggctgg acccgctttc gactgcggcg gccttggcga ggatttcggc
    28921 atcgtcgccc tcgagggcaa tgacatccgt cacgcgcggc cgcccgaccg taagcgttcc
    28981 ggtcttgtca aaggcgaccg tgcgcacttt gcccagcgtc tcaagcgcgg cgccgccttt
    29041 cacgagaagc ccgcgccggg cacctgcggc cagtccggaa gcgatcgccg ccggcgtcga
    29101 gatgacgagc gcgcatggac aggcgataag cagcgtcgct aggccgcgat agacccaggt
    29161 cgaccattcc gccccgaaca gcagcggagg cgcgatcatg atcagggcgg caaccgccat
    29221 ggcggcgggt gtgtaatagg tgctgaaccg gtcgatgaag cgcgccgtcg gggctttcga
    29281 gccttgcgcc tcctctacga gatgaatgat gcgtgcaatc gtgttgtcgg cggccgtccg
    29341 cgtgatccgc acccgcagca cgccattggc gttgatgctg ccggcaaaaa cggcgctgcc
    29401 cggctcctta aggaccggca ccgactcgcc cgtgacgggc gcctcgtcca gctcggacga
    29461 gccctcgaca acctctccat cggaaggcac gcgatcgcca gggcggacca cgacgatatt
    29521 gccgatggcg agccgttcga ccggaacttc cgcaacagtt ccatctcgct ccaggcgggc
    29581 gacgcgcggc acgaggttca tcagcgaagc gatcccggag cgcgcccgtc ctgcggcgac
    29641 gttctcgagc agctcgccta ccgcgaacag gaagaccacg acggcggcct ctgcgccctc
    29701 gccaatcgcg atggcgccga tcgcggcaac ggacatcagg gtttcgatgc tgaagggcgt
    29761 accatgacgt gccccggcga ctgcacgccg ggcgatcggc acgaggccga gacccgccgc
    29821 cgccacatag gcccagtccg ccacatccgg cgcaatcgtc gcgactgcga aggccagagc
    29881 aaggagcgcg ccggtgccga gaacgagctg gcctttgccg gtgcgccacc aggtccgatc
    29941 gacggtctca ggtcggtcag gcgggacatt ttcgtccgcc ataggctcgc cgcccacggg
    30001 gtacggcgtg taaccgagcg agcggatgcg ctcctcgatc accgtgcgcg acgtgcggtc
    30061 ctcgtccaga acgagcgaga gcatctccag tccgacattc acattgatgt cgctgacgcc
    30121 gggcagacgt ttcagtccgt tctcgatttt gagtccgcag gccccgcaat ccatgccttc
    30181 gacgcggagc ttaagcgcac tggctggcgc aactgccatg atgtcacctc gacttgttcg
    30241 atgtgacatc gcatatagac cctgtagcaa ctacagggtc aagatgatga cgcgcgaaaa
    30301 gttctcaatc ggcgatttgg ccaaggcgac cgacacgaag gtagagacga tccggtatta
    30361 cgagcgcata gggctcttgc cggagccagc acgcactagc ggcaattacc ggagctattc
    30421 ggccgcgcat ctcggtcgcc tcagcttcat tcgccgcgcg cgcgatctcg gcttctcgat
    30481 cgagcaggtg cgcgacctcc tcggactttc cgatcagcgc gatcgacctt gtgaggctgt
    30541 tgacgcgatc gcccgggagc atctcggcga agtgcagcgc aagatccggg accttcaggc
    30601 cctgcgccgc gagctggaca gcattctcag ccagtgcgga tgcggcacaa tcgccgaatg
    30661 ccggattatc gaagcgctgt cgcccaacga tcatcgctga gctgaagccc cgcggcagga
    30721 gtatgggggg tcagcttacc cggctgatga tgaaccggct ttggggtaat gcgggcagcc
    30781 gtgagtagtc gatccgcaaa tcttgctccg gcacttggta tctgatccgt ctggtcagaa
    30841 cgtctgacgc ctgcttcatc agctggattg tgatccactc gccagcgcag cggtggtcgg
    30901 cgttgtgatc cccgccgccc tgaggaacga agttgaacgg gctgccgtcc caagcacgaa
    30961 atcgttcagg ccaaaaagcc tcgggtgagt cccacgtcct ggcgtcgtgg ttcgttccgt
    31021 aaagatccag gataacgcgt cgcccgctag agcatgatcc attcatatgg aagcatatcc
    31081 tgagttggag agatagtttg agcattcggt tggagtgaag ttgttgagca gcgatccgat
    31141 gtgccgccag gttgcttcga cgctgcgcct gtcggctttg cgaaggagtg tcttgagctt
    31201 tgcgaagacc tgctcgatgg gattgaggtc cgggctgtag ggcggcagga agagcagctt
    31261 ggccccggcc ctgcgcaggg ccgcgcgcac ggctttgcct ttgtggcttc ccagattgtc
    31321 gaggatgacg atgtcgcccg gggtgagcgt gggcacaaga atctgctcga cataggccag
    31381 aaaggattgg ccattgatgg gtccgtccat cacacagggt gcgtcgatgc ggtccgtgcg
    31441 caaagcggca aggaaggtca gcgtccgcca gtgtccgtgc ggcactttgg cgacgaggcg
    31501 ctggccccgc gcgcagcggc catgggtgcg cgtcatgttg gtcttggccc acgtctcatc
    31561 aatgaagacg aggcgggcgg gatcaagttt gccctgatgg cgcttccagc gttcgcgccg
    31621 ccttgcgacg tcaggcctgt cctgctcgct tgcgtgcagg cttttttttg aaggtgatcc
    31681 cttcgtcgtc cagcaggcgc cagaccgagc catagctcgc cgggatcgca cgcgcgtgga
    31741 gttcgcccac cagtgcgcgc aatgtcaggt ccggcttggc ttgaagacgc tgcagcatcc
    31801 agtcccgctg cccggccaga gagcgcgcat ggcgcgtccc catcggcttg gcggcaacag
    31861 tgccggtctg ccgcttgcgc tgtgaccagc ggaccacgct ggcgacgctg acaccaaaaa
    31921 tcgccgcaac ctcgcggcat gagtgaccag cttccacacg ccgcaccacc cgcttgcgca
    31981 aatcctcaga taacgctcgg accattcatg ctgacctcct gccagtctgc atcttgaatc
    32041 agactcgcag acccttggga atcccaatga ttcaaatcac cagaatcatg ctctagggaa
    32101 gcggtatccc atccaatcga aatcggatcg caccagcgcc gcgacagcgg gaaagaaagg
    32161 gtagaagcgc cgaacctcct ggacgaagag ttctgggtaa gaaccgtcgt cgccgtcttc
    32221 gagctcgctg cggcaatcag gatggtgatg aagggcgtgg gcgacgaagg tgatgtagac
    32281 accgacggca acgatcggtc taatcacgtt gaggacttca acagcggcga cgtgagggct
    32341 cagcagttca ccgtccaagt cccgatgctt cgcaatggtc gaagcagccg atccttccgg
    32401 cgggcgcatt tgtcctgaac ggatctgcat gacaacgttc tcaatccagc gattggcccg
    32461 cttgcgcgcc caacgagctc gccagtgctt ggggccgacc gagcccgcgg cgtcgaacag
    32521 cgcggtgatc tcgtgcgttc gctgtggcac ctgagcctcg ggtagcggca ccccggccca
    32581 cgcgcacgcg gctcgggtca ggagttcgtg aagctcgtcg tataagacga tctcgtcctt
    32641 ctgtgcccat aggcgagcac gggcctgcca ctcctccgca gtagttgcta tcagtccttc
    32701 gatccgctcg ggcgtcatca gggacatgaa catctgcttg cgatgtcggt gagccgcgtc
    32761 atccagccct tgcacaccac ctcttccgag cagcgttttc tggattcgcc cgatcatggc
    32821 gccctgccgc ttaaatcggc tttgatcgta gaaaagccga gccgcctcgg gccccgtcat
    32881 acagattgtc ttgccgagca taagccgggt ctcgaacagg tcagctccat gggcgcggca
    32941 ctgcttcgag atgaacctgt aagggtcctt gagcagcgcg agcgtcgcgt cgagccggtt
    33001 gtcacgaggg agttgcggca tcaaggcgcc ttcatcggac caacatcggg gtgcttcttc
    33061 ggacccttcg cctcgtctct gggcaagatg ctttcgagca acagaaggcc gacgccgctc
    33121 acgatcgcca catcggcgag attgaaagcc ggccagtgaa aggtggcgag gtagaagtcc
    33181 agaaagtccg tcacggcgcc atgccgcagc cggtcgacga ggttgccgac cgcgcccccg
    33241 gcgatgagcc cgagcgaaat ggccgtccgg cggtaatcgg cccgctgcat ccaaacaatg
    33301 agcccggcca caatcgccag cgtgagagcc atcagtgccc atggcgaggc gtcgccaaac
    33361 agcccgaaac tcacgcccgt gttgcggccg aggaccaggt tgaagaagcc ggtgaccggg
    33421 atcacccggg gcggggccat gacgtggttg aggatcagcc acttcgatac ctgatccgcc
    33481 gtgaaggcaa tgaatgcgat ggggagccca agtttcagca tgcctgtcct attccgttgt
    33541 tgggctcaac cgatctggcc aagagccggg aaggtctgca ggagccagat cgcgaagctg
    33601 ctcagttggc cggtaatcat cgcgatgccc attactacca ttgtcccgcc ggcaacgatc
    33661 ttcaggacgc gtccggttcg ccgcatggcc gtcatccgcg ccatgaaacc gcgcatgaac
    33721 agggcggcta ggatgaacgg caggccaagt ccgagcgaat agactgccag gagggccgtt
    33781 ccgcttccgg ccgtggcgtt cgcggccgaa agcgccaata tcgcaccgag aaccggcccg
    33841 atgcagggcg tccagccgaa ggcgaaggcg aggcccagga ggtagtttgc ccaaggcccg
    33901 ccgccatgac ggcccgaatg aaatctcaca tcccgctcga accacggcat gggcacgagg
    33961 ccggtcgtga acaggccgaa gacaatgacg atcgcaccgc cgatcagatt ggcctcatag
    34021 aggtgcgcgc gcagaagccc acttagcgct gtcgcgctgg ctccgaggag gacgaatacg
    34081 gtagtgaagc cgagcacgaa gcagaggccc aaaccgagtg ttcgaaccgt ctccttcagg
    34141 cctactgggc cggcaagccc atccgggctg atggtccgcc cggcgacata ggacacatag
    34201 cccggcacga gaggcagaac gcaaggggat aggaacgaga tgacgcccgc ggcaaaagcc
    34261 gtggctatac caacgccgga ggggtccact gcgatcctcc tccgtcaggc cttggcgccg
    34321 ctcgccgatg cggcctctcg aagatcgttg cgggcatcgc ggatgatcga ccacgccgac
    34381 tgcaggaaca gcccggccac ggcaaacgcc acgatcagat ccggccaggg cgaattcagc
    34441 gcccatacga cgccggccgc gaccactacg gcgaggttgc caattgcgtc gttgcgtgaa
    34501 aacagccaca cggcgcgcat gttggcatcg cccttccggt gcggcagcag caccacggcc
    34561 gcgagcacgt tgacgatcag ggcgacgatg gcaaaacccg ccatcagcag cgtttggggc
    34621 tcgtgctcga cgaagacgcg gtagaacgtc gagccgatca cgccaaagcc gagcagcgcg
    34681 agaaagattc cctgcagcag tgccgcgcgg gctcgcgctg caaggcccca gcccacggct
    34741 atcagcccaa ggaacgagat cagaccgtcg ccgatgaagt cgagcgcgtc ggcctgcagc
    34801 gcttgcgagc ccgcgaggaa gctgccgccg atttcaatga cgccgtagcc aacgttcagc
    34861 agaacgacga tccatagggc ccgcttatag gccggcgtga catgcgacag atctgggatg
    34921 cggtcgtcgt catcatcctc ggcggcatcg gccgaagcct tggccgcgcc gatacggtcg
    34981 agttggtaac cgagctcgct caccgcattt tcaacgacgg gcagtcgggc ggctggatca
    35041 tcgacctcaa gcgtcatgat ctgcgaggcg atcgacacct ttacatcgga gacaccggag
    35101 accttgcgcg ctgcgccttc gattttggcg gcgcacgagg cacaatccat gcccgtgacg
    35161 tgatagcggg ccgtggcggg agcagtcatg gattcggtcc tttgggctcg aagggaatga
    35221 tcgggcgaac tttgccgcct tgggtttgtt cgggcggctc gtcgccgcgc tcgattcgga
    35281 cgtggccgag accgtgtcgc tcgagcagtc cgcgcatatc agcctcgacc cgcggccata
    35341 cggtgtcagg attgccggag actttcaggc gcacgcgcac cgcatcgggt gccgtctgca
    35401 ccacctggta gagatcgacg ccctgcactg gatcaagtag ggagtaaaag accattggcg
    35461 gcatggtcac ggcctggccg ccgcggccgg cgaagcgcac tagttcggag gcgcgccctt
    35521 ggactcggat tgccggaaaa gggctgccgc aggggcaggc gtccggccgc tcgacgatgc
    35581 tgtcgccgag atcgtagcgt agaacgggct gcagggcgtt cgccagattg ctcaccagga
    35641 ccgtgtgcga gacatcccct ttgggtactg gcctgtggct cgcatcgacg gcctcgaatg
    35701 tcgcccaatc ggagttcacg tgcagccaac catgagggca gctgaagctc aaaaacggca
    35761 cttccgttga agcgtagcta ttgcccacct tggcgttgaa ggcgcgcgcg atgcgcccgt
    35821 attcttccgg agctaagccc tcggccgcaa gcgcgagcag cgcggggcga atatgcagcc
    35881 ggccctgctc ctgttctgtg gcgagtagcg ctgcaatgct cgcgtaaggc gccacgagca
    35941 cgggctggaa atcattaagg gcggccacga tctcccggat tggatcatgc accgagagct
    36001 gcagcagttt attcttgcgc cgacgcttca acgccgcggc ggccacagcc gacgcgaaat
    36061 gtccgccctt ggccatcacc atcgccatgc gcccggagcc ggcgatgatc ttcacgaaat
    36121 cgccgaagct taaccagctc gtgagcatgc gaaaggcgat cgactgtgtg accgccatgg
    36181 cccgatcgtc ataaacgaag atgcctttgg tcccactcgt ccctgaggtg gtcgccaggg
    36241 tgtagccgtg gaacttctcg ccgacttttt tcaggtcttc ggcgaaggct tgtgcgtcgg
    36301 acaacctgat cgtccgatcg gttacccaat cgtcgaatcc actcatcagc cacttcttgg
    36361 tggtgatcgg catgttctcg agcctaaccg gcccctcggg tatatcggca taaagacgcc
    36421 ggtagagggg ggaattggcc cgagcgaaag cgaccattgc gccgagtcgc tcacgttgcc
    36481 gtcggacaat gccttcggcc ccttccttcc tcgcgcggcg gttatcgatc acgagcccaa
    36541 gcaggttttc acccatgcga aggcctcctt tctcgatatg ccgaacgcgc ccttgcggtg
    36601 atatgtgctc cgaatctaca acctgtagcg actacaggag caaggactaa aaatgcagat
    36661 cggggacctg tcgcgcgccg ccggcgtcaa catcgaaacc atccggtatt acgagcggat
    36721 cggcgtgctg cctagggcgg cccgccaggt gaacggccgg cgtacctact cccaagacga
    36781 tgcgcgccgc ttgggcttca ttcgtcatgc ccgggatctt ggatttgatc tggccgacgt
    36841 gcgcacgctt cttgccctgc aggagcaacc cgaagcctcc tgcgaagatg caagccgtat
    36901 cgcccaagca cagctcgagg cggtcgagag ccgaattgcc aggctccaga gcctgcgcgc
    36961 cgagctcacc cgcatgctga cggaatgtca gaatggggtt gtcgcccaat gtcgcgtcat
    37021 tgaagcgctc aacggctagt aggaggaatc agtaggaggc ccgtctcgct tccgccgcgc
    37081 cttcgcaacc tccgctgatc ctcatcaacc ccggcaggct ctcgcctgcg ttcgcattac
    37141 ttcgtagtcg ctcatcatgg caacgatcgc tgatcgatca ggcagaagag cgagttcgtc
    37201 ggctgcttgc atctgaagct cgcggctgta ctccacgacc ggcggacaga tcgaaacgcg
    37261 gagcgggtca gaaccgaccg tcgcgcatgc gctcagcgag gtcgtcacga tcgcgagggc
    37321 ggcgagccgc cgcctcaacc atgcgacgtt ggatttcatt ggccttctcc gtggcttcta
    37381 ggcgttcaac gagacgacct gctctctcgc cggaacgccg gagggagaac aggaacaaca
    37441 ggacggcgag ggcgagagcg ctgtatcgta gggcggtacg cgcccaggga ctgctggtga
    37501 tcgcgccgaa caaagccgtg atcatcgccg tccccgtttc cagtcgtcga tgcgggcata
    37561 gatcgcgacg gcaatgccga taagcgcgac tgcgatgaac acccagcgca gcgtgtccag
    37621 gtaaggcagg agcggcagaa tggctgattg cgtctcggtg aggatactct gcgccacctc
    37681 gacacccgcg gcgcccagcg tggccacgcc agctgcgccg ctgcccttca tcgtgcgact
    37741 gtcggccaag acctcccgca cgggcgccgt ttccgcggcg aacgccatcg cctgcaccgg
    37801 gaagcgtgcg ccccattgcc gggcgggacc gagatcgatg tgcatgaagc ccgagcgcgg
    37861 atagtagccg aagccgagaa agccaacggc tcgggcggcg acctcgaatg ccaccggatc
    37921 gtggttcgcc atggcgatgt cgaaggccgt gccgtccatg tgcttcgagc gcggcgcgcc
    37981 gccgacggcc cggttgtgcg caggactgcg gtaggccgaa cggacgataa gcggcttgcc
    38041 gagccgatcg cggagcgcct ggagcttgtc gagcgctcct tcgttgatgc gcaaagagcc
    38101 gctgccgcga cacgcgatct ccgcgggcga gaaatttttc cagcgccagg cgccctcggg
    38161 cacgtcgcgc cagtggttaa agtagctcgt cgtcatggga ggtctccagg cacaaaaaag
    38221 cccgctcctc atcgagggcg ggcgcggtct cagatgtctg agtgatggct tacggcgacg
    38281 gaccgaagag cttcagcttg atggcgatgc cggccatcag ggcgagcagg atgccggtgg
    38341 tgatcagccg cacagcggtc tgaactgctg tctgcttcgc aagccgaaag ctggacagca
    38401 atgagcggag atcgcggatg tcttcggccg catccgctcc gtcaaggccg acctcatgca
    38461 gcgcccgccg cgccccggtt tcggcggcgc gctccaagag cgcctcgaac tcgtcgctcg
    38521 gcaggctgac catcccgccg ctttccctat tctgatccat gcgtctggtt tccgtggcag
    38581 tggtcagacc accagcgccc cagcgagcgt ggcgccgagg tcgaccaagg tcgcatcggg
    38641 cgtcgatggc gcgacgatgc tcacaggata tcgcctggct caaacaccgt tttcaggtag
    38701 acgatcgagg cgtagctcgt ggcgacgtga agcttgaagg cggcaagttc atcgcggcgg
    38761 gccttgaggt cgcgttcatt cgccgcgcga tggctctgca gaagccaaga caggccgcca
    38821 agagccggta cctggatggc cgagacgcac caggtcagat ccattgaaag ccccacgcgt
    38881 tcgaggatcg gagagatacg ccgccgccgt tagccggtaa cgacgcaaac gatcgtcccg
    38941 ccaccggcgg tcgccggctt gtcagcgacc gagcggatca gctccgcgta gatctgcgcg
    39001 gcgcgccccg tcgatgcggt cgcgaccagc gcctcggcat aggcttgcgc cgcccgcgcc
    39061 gggctcggcg catcaagcag cgcctctgca tagacttgga cgagatcgcc agctggtggc
    39121 gtgctcagca ccagttcggc gtcgacgatg tcgaagtacg aatccgtcga ggtcccagta
    39181 acccacatgc cccctaagat caggcgcagc ttgcgcgtcc cggcgggaat cgccggtgtg
    39241 agggcccgca atccccaagc ctgagcgctg tcagggaaat tgccgctcga tgccgtcgag
    39301 attagcacat ccgcgacatc gagaaacgcc acttcgacga atccgatgtc gttgccagct
    39361 gcgaagttgc tccagagaac gcggagcgaa accgacagca gcccggcatc gacgtcagtc
    39421 aggcaatccg ctggaaccac cacatcctgg cgcactcggt aggagccggc gctgccattg
    39481 ttggcgacct gcaagtacct gctgccgccc cgaatatcgg tcggcggact ggtggtcttg
    39541 aagactgggg ttccattgac ggtcgtccaa ccatcgccgg gcgcaacacc ggtttcgaag
    39601 ctcgggttgg tgaccgcaag ccgcttcggg taggtcggca tgacggatca ggcccccagt
    39661 tcgatgccga gctgcgctgc gttgatcgtc gagcccgccc agggtccacc accatccggg
    39721 ttacggtaga ccggatgctg caatgtcgag aactgattgg agagcgcgag cccagcattc
    39781 aggtcctctg cagcattcac acgaatgaag ggtttgagga cccgcgcgcc aacatcggtt
    39841 cggcggcctc gaacattcac gacaagagca tgggtcgtct ggggctcagc cacggggtcc
    39901 gtcatcgtga agatctcgcg agcacccgtc gccgtcgcag acaggaagtc ggcatcattg
    39961 ggcgccgcct cgtcaatcag cgcgaagcgg tcggttccgg tcgatggcgt ccagccgccg
    40021 cttggctggt cggcggtggg ccgcagagtt tcgatcttga ggtcggatgt gaggatcgcg
    40081 ttgaggtatg acccggatgc atcatggaga atgaagtcgt cccaatccca ttcgagtcct
    40141 gcgccgccac gcggtgtgcc gatgattacc cgatcgatgt tggggcctga ggtcccggaa
    40201 cgcgtatcgc caatgagatt gaattgctcg acgccattca aaatcaggcg ccagcgcccg
    40261 ccgctgtcgg cacatttgaa gtgcaaggcg atcgtgttcc attcgccaag cacgatcgag
    40321 ttcgcggagt tcgaggtaaa ggcggccgag gaccccatca atgttccgaa cagggcgcca
    40381 cccgttgtga tgcgcaagga agcgccattg ccgaggacat cctcgaacca taggaatggc
    40441 gtgctggtcg tcggataaga tcggggaaag atcgccgccc agacgaacat ctcgtcgacg
    40501 gcagggttgg tctttctcac cagcatcccg gtcgtcgctg agaaccgcac ggctccaccg
    40561 ccaaaccgcc cgccagtcgg cagaaaagag aggtcggcgg cgaccgagaa acggtagctg
    40621 ccccatttcc cgaccgtatc gccgatcgcg ccatagctgt cgaagccgtc acagaattgc
    40681 agcgccataa ttatccatcc ttgtgcatta ggttctcgcg ccgacaaacg agagagagat
    40741 gtccgcgagc gtcgcgtcct ggggcgcagg cgcgagcagt tcgagcacgt ccccagctgc
    40801 gaagttggtg ccgcctgcca aagtgaagct ggcgactgcc tgaccggcgg cgaaatcgac
    40861 ggagccgacg ttggtgccgt tcttgcggag cgcgaacgac gcgaggctcg tcgcccctgt
    40921 ggctgcccgg ccggtactcc ccgtcaggct tgtcggaaaa ctcacgcctc tcgggaacac
    40981 gtactgaaag acgagcgcag tcgcagcagg caggccagga atgaacagcc caaggtcgta
    41041 aaggccgcct attgccgagg ccgcgagcga aaccagattg gtgccccgcg agccgacgat
    41101 cccgagttcg cttgcccgga ggatcaccgt ggcgcccgcc gttgtggttc gcagcgtggc
    41161 gatccttgcc gtcgtgttgt gcacggccaa gattcgcttg ccgtccggga ccgtgagcgt
    41221 gaaatccgag gccggcgtgc cggtgaggcg gatcaggaag ttctcacgat aatccgacgc
    41281 tgaaaccgtc gtgttgccgc cggtgcaatc gacgtccacg gtgtcgttca tcgccagatc
    41341 gagcctgtcc agcgcatcat tgatcgtgac ctccttctgg ttttgcgagg ccgcgacatg
    41401 ggtgatggcg agattaggcg tcggcattca ggcaacctcc acggtgacgg cgcgcgcaaa
    41461 tcctcggccc gcgatcgcgc tcagctgata ggcggcgacc gacagcgacg tcggcacggt
    41521 gccaaagtcg gtgaggatgt cggcattggc gtagacgaca ctcggactgg cggtgaccag
    41581 cgtgcgtttc aaggcgccgc cggggccagc taggatgtcg atctcgtagg cctcgctcgc
    41641 ctccccaagc ggcaccacgc cggagccgtc tttcagctcg ccgccgatgc gcgtgcgccg
    41701 aacccagctg agcgtgatat tgctcggact gccggtcttc acggcgcgaa ggttccaagg
    41761 cgcgtagggc ttcagatcgc ggccggtatg ggcgtgggat acggtctcgg cgtcttcgaa
    41821 cagggttccg aagccgagcg ctcgccagag ccggctcaag cccagatcgc caagcgacag
    41881 gctcagcgat tcgacatcgt cggggtcgag aagcacgaag agctcgccgg gtgcgtggcc
    41941 gctgacgaag acatcggtgc cccgccgtcc tcgcagcagg ccacgcaagg tataagagcc
    42001 atccggattg agcgtgacgt cccggaactg gatgacctcg ggctcgccat tggccttgag
    42061 caccagtgct gcgttcgccc cattgaccag cgcctcctgc gagacgctct ccaggcgatc
    42121 gctgcccgtg gtcatgaaca cggcgagcac gttcacttca tccgttgcga agggcgagct
    42181 gggactgccc agcgcattgg ccgtcgcgcc ccaggccgct tcgctcaagg cccggccgac
    42241 ctgcgaccag gccgagccgt cgagacttcg gtagagcgag ccgccgggcc agccggggcc
    42301 accaaagcct cccatcaggt agtaggcgcg cgagccggtc tgcccgagat cgtcggtgtc
    42361 gcgcagcaaa ggcagatcgg gaaggatcag cttgctcgcc gcctgcgcag cgatcacttg
    42421 ggccggtttg ccggcgccgc tatcggcaag aaccgaggag acgtaggtcg cggcggtctc
    42481 ggacacgccc ttgatcccga gcaggaagtc ggcgccgaca tcgatgcggg tcagacgtgt
    42541 cctaaaacca gagccggtcg cgaacacgac atcgattacg tcggtcgggt cgagccgtag
    42601 ccagtcgggt ggcagctgcg cctcgtagga ggagcgctcc tcccaggcgc tgaacagggt
    42661 tttcgcggca atgcgcttgg cggtggtggc atcgatggcg agcgccagtt cgaggctcgc
    42721 ctgattgcgc gaatgcatgg tcggcgtcgg caaggacgcc cgcttctcgc tctgcgagcc
    42781 ctgcgcatag tcggcggcgg tgtccatgta gacgatgctg acccgctcgg gcagctccac
    42841 ctcttgcgtt cgccgctcgc gccaggtttc ggcggtccga tcgtcgatcg gcacgaggaa
    42901 ctggctgtcg atcgtcgcga ccggcgcccg gccgcgtgta cggaaacgca gcacgtcgtc
    42961 gctttcggcg gcgtcgaaga aataggcctg cgccagcggt tcgatcgcgc cgcgaacggt
    43021 ggtctgccgc ccgatcacgt agccgggcac gatgtcgtcg agttcaccga gatcaaggtc
    43081 ggccagccct aagcccgctc gctcgcagag gtcggcaacg ataccggaga gtgtttcgcc
    43141 ttcaccgccg ccgcgattga gaaacagccg ggcccagcca ttggcgccac gcaccagatg
    43201 cgtgtcggtg atcgcgtcat agacctgcgc accggtttcg ctaaccgcgc ccggccaggt
    43261 ttcgtcggtt acgagggcgc ccgtcgcggt atcgatctgg acgagccgcg tcgagcgcat
    43321 cagcgtccag tgttgaccct gaaggcggct ctgaccgaag aatggcccct cgtagttgat
    43381 ctggatcggc gtcggcgtct tccagacgat cccggcatcg cttcgccatt tgaccgtgta
    43441 gatcgcgccg gccgacccgc cattcgagat ctgcacctga aagatcacgc catcgtcggt
    43501 cgcgtcatag gtgaggccgc cggcatcgtc atagaacgcg gttgcccctg cttgaatctg
    43561 cgaaggcgcg aagctcgcga ccttctcgaa agtcacgccg agactaaggc cgctcaagga
    43621 atcgtaggcg gctgcagcgg cgacacgaat gcgatagagc gcaagcccgg tgtggctcgt
    43681 accggtgccg ctgccgagaa cccagccctc gccgaaacct tcgccgactg cgccgccgat
    43741 cgcgccacgg acgcgcgact catcgaccgt ttggcccgcg ccccagacat aacccatgcc
    43801 gtcggcgcgg atcagaccga catcatcgaa aagcgaaccg gtcaggatga agtccgcccg
    43861 gcccgacggg ccataggcgg agaccatgcc catccatgtg gtcgccacga accgcagcgt
    43921 tgagttcgat agcccgctgc tggtcgagcc gaaacgactc acctctttca gagcattggg
    43981 ctcgacgcgc aggatcggtc gggaattgct cgatcccgtc acgagataca ggtagccatc
    44041 ctcgccgcag aagagcgtgc tcgggaagtt gttcggcgaa acgctggtga cgtcgctcat
    44101 gcgcgcctgg cggtcctctt tcatcgtccg caggctgaag cgccggatgc ccgcgttcga
    44161 ggcgctgaca tcgctgtcga tgaagtagcc gtagccgcgc cgccaatcga ccgcgagctc
    44221 gtcgatctga taggaatcga gaaggccgcc ttcgcccgtg gtgatgaaat cgagcagttg
    44281 atagggcctg ctctcggcgc gccggaaggt gatttcggcg gtgatgttcg ggatcctgtt
    44341 cccgaaatcg gccaaggcca ggtcctcaaa aacgatcgtg cacagaccac ggtgcgcggg
    44401 cgcgcggccg gcgccgacat gggtttcgat caagggatcg ggcaactggg tctcttcgcc
    44461 cgcgtgaaaa cggaaccgta gattgggttt tgcgacatcc gcgctcgatc cggtcttgtc
    44521 gtagatcagc ttgccgtcgg cccagatgcg caagacatct tcggccggcc cctcgccgaa
    44581 gctgagggcg aacgaggcga agtaggaata ggtgaccgag gtctgggtgg caccaccgct
    44641 gccgccctta cccccggaac gggtacgggt gacgttctgc tgttcgcgga tgccggacga
    44701 ccagatgaca ttgccggcca tgcgcaaggt gccataggcg atcgcgatcg gcgcaccgta
    44761 agccgaggat gtgaccgtca ggtcgccaag gcgcgggcct tcggtccgga cgtccggacc
    44821 gcgacgcggg aagatcaggt tgccgacgac cgatccgacc agccagcctg cgctccagcc
    44881 cacgccgacg gccgagccga gcacggcgcc gccaaccgcg acaaggatgg ccatgggcta
    44941 ctccgaaact ccgcgaaagc ggaaggcgaa tttgaccttc gcacgccatt cgctggcgta
    45001 aggctcttcg atcacctgcc gtctcagcgc atgcgcatga agcagatgag gcgtaccgaa
    45061 gcgctcggtc agaaagccgc aatggcaggg ataggcctgg tcggcaaaga ccagcacgtc
    45121 gccgccgcgc acctcggtga cggcgacact atccatctgg ctgcggaaat gctcgacgaa
    45181 gccttggcct tgcgcccggc ggccataggc cgtgctgtca taatccgcca gccccagatc
    45241 gcgcgcgacg aggacgacca ggccggcgca atcgatgccc gcacgcgtgc gcccttgatg
    45301 ccgccaggga atgccgagat aggaccgcgc ctgggccacc agccgatcag ccgtcacggt
    45361 ttcatcgtgc atcgggatag gtcatgagcg agtcctgccc gggcacgtag ggctcacccc
    45421 ggaagttcag gacattggca aagcggtcga tacaggtgtc gaggcgcttg tcgcatccgg
    45481 ggtggattcg gaaggcgtcg ccgacccgga tcgcgtagcc catggcgagg aacagctcga
    45541 ggcgcgcggt gctcgcggtc caggctttca cttccatcgc gcggccggcg ttgttgccag
    45601 actcccaggt cagcacgccg ccggcgaacc agtcatcgac ggcgcgaggt tcgtcgagca
    45661 cgacgaacat actggcgcgg tcgatgacat cggcgacgat accggaccgc gtccaggcat
    45721 cctccgcgat aaagaccgcc gtgccgtcgg tcgtctgcgc gccgaccgtc gttgtgtatg
    45781 ccggctgcgt gaccgctgtc gtgcccgccg tggtgcaacg gtagatgcgg ttttcgaatg
    45841 cggcggatgt cgcaacgacc gcggtcgtgt ccgagaagtg tccttcgatg ccgtcgagcg
    45901 ccgcgttgca cacggtgcca cccaggcgcg tgccgttgaa aatgacgcgc agctggcgcg
    45961 tccccgccgg cacggggaca tcgacggcct ggcgaagcga ccaggacgtt ccagccacct
    46021 cattaccggt atcgagcggt gtcgccagca cggcacctgc ggcatcgaga cattcgaagc
    46081 gcagacgccc ctgatcgacg agatcgttcg agccattggc ccgccagccg cgcaaggtta
    46141 cgagataggc gccgctgtcg gtcttcgcgg gatcgatgac gtcggtcaga tcgaccgtct
    46201 gacgcacctc gaaactggcg atgctgccgc cttcgagaaa gtgcgtgcgt gtcttgggtc
    46261 cgagcgggga gctgatcgtc ttggctgtcg ccgtgccgga ggcgaccgtc cagcccgaga
    46321 gactgccagc atcgaagccc ggattgacga acgggttccc gatctgcgcg ggcgcggttg
    46381 acgtccggac acgaacgaca tcgccagcgg cataggcagt cgcgcgcgcg atctccgccg
    46441 gcaggatcgg gactttgcaa cgatgatcgc cgagatcggc gcggcattcg gggctgtaga
    46501 gctcgccgat gcgctgctgc agcgcctggg tcatgccgcg caactccgtg cggaaggtgc
    46561 cctgctcggt gagcaggacc tcgccgaacc agcccctgcg catccgcaag gggccttgcg
    46621 ccgggtccat ccagttgacg agaaagatgc gaacctcggc gccgtcgaac agcccagcgc
    46681 gaagttcctc ctcacggatc gccgtgctgt cgaagacacc ttcgacatcg agattgtcga
    46741 cgctgaggct tgcgtcattg gcgatcgccg tgcgcgaata gccggtgctg gcgcgatagg
    46801 tgctgccttc gaaatcgatg tcccggtcgt ggtcggtgaa atagaactcg acgccgtcga
    46861 gacgggtgat gcgccagagg caggcgagcg tcgtcaccgg ccctgccaag tgggccgcaa
    46921 gcgccgtgga gacggatttc atgggcggat ctcgatgatg gggatctgcc cccagctgcc
    46981 cagctgatag gtctcgatcg tgatgtccat ctggtcgctg tcgaaacggg ccggcacatc
    47041 gaactcgaag tccgccgtca cctggacgcc ggaggccggc gccgccgtga aggtgacgag
    47101 acccgtcgtg atatcgatgc tccagccgga cgtggcctcg atgccgttgc gatagagctt
    47161 ggtgctgccg gcgaccagct tgacgatggt gcgctgctcg acctcggcgc cgctcgcata
    47221 gtgcttgacc agctgaaagg tcttggtcgt gccgtcgccg accccgatcg cttgcgcgag
    47281 gccctggtag tcggtccagt ccttgaagcg gaagccatag gcgcgaccct tccgcgcccg
    47341 aaagaacgca atcaaggctg ccacctggtc ccgcttcttc agcccgtgcg ccacattcca
    47401 gcggccgcgc gcctgcgccc aattggcgtt gcggcgctcg tggcccgaca ctgtggtcac
    47461 gaccgtcgtc gagtagccgg gaccgcccgc cgcgccatag gagatgtcgg gcgggaactg
    47521 aacctcgtgg aacccggcca tgatcgccct ccgtcagaga ttgcaccggg cccggtcgat
    47581 ggcgcgcgcc gcctcggccg cgatctgccc ctgcgcatag cgaaagccgt tggtgtccgg
    47641 cgtggagata ttcatcacca cgttgatcgg ttgcgaagcg cggctttccc ggcccatgga
    47701 gccgcccagg gcgcgcatct gttcgggcgt cagaaccgcc tcaccgcgct tcaagatggc
    47761 tggtagttcg tcgggcgcga ggcccgccag tccgccggtg tggtagcgcg gcgcggtcag
    47821 gaagagaccg gcatccaccg aacgcgcggg cgaggccgaa tggccgatca cgccaccttc
    47881 gtggaagagg cccgcgaaga tctggccgat gccgcccagc agatcgtcgg cgttatcgtt
    47941 caagaatcct gcgagtggcg cggtaatcgc ctgacgcacg gcgatccgcg tgatgtcggc
    48001 cagaatgctc tcggtcagcg aggagaactc gaacttgccg gtggtcacga aggaaacgag
    48061 cgcgtcctcc atggactgga aggcgagcgt ggttgcctct tcggccgcgc tcgcggcgtc
    48121 ggtcgaggca tcgacataat ccttaagcgc acggcgcacg ccgtcctgcc attcgcggct
    48181 ctgacgcagg gcgcgctcct gcgcctcggc aaggtcctcg ttggcccccg cgagcgcccg
    48241 gttgaaggtc tcctggtcga ttgcgcctgc gcgcaggagt gtattcagcc gctccagcgt
    48301 ggcggcgtac tcttccgtcg gcgttcgcgt ctgctcgatg agccggcgcc cttcctcgcg
    48361 cagccgctct tcttcgcgca tcgctttggc gagctcttcg cgagcctgtt tctcgtcgta
    48421 gagcgcgccg gccagccgct cgacctccgc gcgctgggcg gcggtcgctc cctccgacaa
    48481 gcgcgacagc gcctgatcga tggcggcctg ccgctcatcg ttgagacctg ccacctggcg
    48541 gcgaaagtcc tcgatgatgc gggcattggc agctccggca cgggttgccg cctcgcgcgc
    48601 cgggcgttcg atggcatcga tacgccgacg cgccagttcc tcggcgcgcg cgatcgccgc
    48661 gtcgacatcg gaggcattgc ttccatcggg caggcgcagc gcggtcaagc gccggcgcgt
    48721 ctcctccaat tcccgattga tgcgggcgat ccgctcaccc ggatccgtcg cgatctggtc
    48781 gatcacccgg tcgatgtcgc ggcgctgtgt cgccagaagc tcggccagcc gctcggcctc
    48841 ggcggcccgg cggcccgctt cggcctggcg ctgctcggcg gcgaagctct cggcctcgga
    48901 gcgggcctgc gcgatcagat catcgacctg gcgctgcagc gcttcgaccg tccgacgctg
    48961 ctcctcgatg gcgaagcgct gcccgaggag gggtgtgccc gggccgccag cctgcaggcg
    49021 ggcaagctcg tcctgcgctt cgatcagacg tttgttgagc gcgacgatcc gcgtcgcgat
    49081 cggatcgtcc tcgaacaggc tggcgatccc ttcggcggcg tcggcgagca aattgagcgc
    49141 gccttccgcc aaacctgaaa cagccggcgt ttggccgatg gcctcaagca gattgcccca
    49201 ggcatcggag agccggttgg cggcgcccgt aaggccgccc gcctcggctg cgcccgcacc
    49261 gccgacttgt tgttcgagcg catcgaggat gaccttctgc gcctcagccg tctggccggt
    49321 ctcgaccagc gactggatga gttcacgctg cgaggcggtg aaagaaacac cgacccggcg
    49381 cagcgccgag atgccctcga cgggttcctc cagcgccttg ccgagttgcg tcgcggagga
    49441 tcgaagatcc tgaccgaaaa cggccgacag atcctgcgcc agccggagcg cccgcgtgaa
    49501 ggtatcgccc gagaccgagc ggaaggtcgc aagcacggcc gcggcgtcct gcacggactc
    49561 ggcggtcgtc agcgtcgtgc gttcgatttc atcggcgaag gcggcaatgg agcgcgcggt
    49621 gaggcccgat gcctggccgg tggcgcgcag caccgcttcg aggcggcgat aggagcgctc
    49681 agcttcggcg gcctcctgca accccgccgt caaggcgagc ccgatgccgg ccaaagccgc
    49741 gccggcggcg atgccagccg gtcccagacg ggtgagcgcg gccccgagcg gaccaagacg
    49801 tcccgtcatg gcctcaagcg agccgcgcac ctggcctgtc gcgccgtcga tcgcctgcaa
    49861 ggcgcgcgag gcgggcctgc tcgcgtcctc gatgcgcttc agcgcgcgtt cgccggtctc
    49921 gccgacatcg cgcagctccg ccttcacctt gccgccgtcg atcacggcga ggcggacgga
    49981 aagattgcgt tcagccacct ttttacccga agattagcaa gtcggaaaag ttatgagcag
    50041 tgcgtgccga agaggcgcac tactcatgtc gcttacgaaa gaaacaaaat gcctgcccct
    50101 gagctgcgca tcggaaatcg aacagcgtat cttcgcaaag cgatcaggac gccagatttt
    50161 caaagtgaaa gcgtgttgac tcgcgacgcg tttcagtttg ctgaactatg gttgcgccga
    50221 aattgcagag aagcgttgcc attctggcga caggcagcaa attactattc cgcgtcacga
    50281 aatttgcctg ccgtatctgc cccactcacc agctattatt gctttctgaa cgccacaaaa
    50341 gcgctgttga ctgtcaaagg tgttgccttt tcggaaaggc acggcgtttc tggcgaattc
    50401 aacccagcag ccaaaagagt tctttcaaac gaggttgtga agattcaatc ggctggaata
    50461 atgacagcgc tatccagtta catggaagaa cccgaaaccc agaacgaaca tgcgctaact
    50521 gatctgctcg caaatctccc cttcatacat cgctcctatc gacacacatt caaatcacag
    50581 aaggaattgt tcattccagt gagaaatgtg gtctacagga aacacccaac tgatgactat
    50641 gtttggttca ctgcggaaat tgtcggaaga tttgccgaca aacgtagcct aagaacgcta
    50701 ccgccacgtt ttgaggtgga tgcaggttat gatgatcgct gcgtcatccg cacgaaaaag
    50761 cggatcaagt ggtttcgtcg aggaactcca aaagcatcca aagctcaagc gttagcacgc
    50821 ctgaacacac tgcatcgctc cgtcagactc gatctgagct tcatttctgc gcctctagac
    50881 ctatggtact tacgtcggga ggtcagcgga aattcgatca ttcgtcgcta tggcatgacg
    50941 atgataatgg caataatgca ccgcctaagt gaactttcga ggtacgaccc taaagggcta
    51001 tcgggccacc tagatggaca agctaactgg ctcctgactg agttcattca gctcgcccca
    51061 gctcaattca tcgatgagat tatttgcgaa atgacatcgt tggagtttcg ccttcctggt
    51121 gtgcgacctt aggacggaca ggcgaaatta cgtggattcc tgagccaacg agaacctatc
    51181 attcaaagcc ttcaccaatc ccgcttcccc tgcaggcaac agctcggcga gagcctgctc
    51241 gccgtaacca gctgcgcggc ccaattggat cgcggcagcg agatcgagac cgatcacgcc
    51301 ggcgccttgg ggcgccaggc gaagctgtcc actgcagcgc gtcagcacgt cccaggccgc
    51361 ccagcccgcg tcgctcagcg gctcatgggc aatgtaggga cagcaattgc cgtcgatgcc
    51421 cgcctcgccg cgcgcacagg gacgccggtc gcgccggcag ccttcgcaat aggacggccc
    51481 acccccgaaa tgccattggg cgcgggccgt cagacgtttt tttcagcgtc cagcagaagc
    51541 gcgggtccca ggtagttccg ctcgaaggct tcggcgatcg gccagaggtc gaggagcgcg
    51601 tcgagcccct ccggcgtgac cgcgaccggc tttccctccg tgtcgccaac cccctcccag
    51661 tcgtcgacgg caagccgcgc cagcgccttc accagcgccg ccgtgcgcaa gccgacggag
    51721 gcctcgtcgt cgatcggcac gccgacgttc gcgcgcgcgg cggcggcgcg ggccgccatg
    51781 accagcgcgg tcgagcaggg tcgcacatgg acgcgaacgc catggccgag atcgagccag
    51841 tgcggctcac gcttcaagtc cagccggatc atgagaaccc tcacacgtaa gaggcaacgt
    51901 cattcagcag gcgcgcgcgc agcatggtgc cttgcgtctc atcgaaggcg gcgcgccagt
    51961 cgaagctcgc ctcgaccccg ccggggccgg agacagcgta tttcggtttg ggaaggaaga
    52021 cgcggaagag ctcgaacttg agttcccagc cctccgccat ggtaaaaccg tagtcgacca
    52081 cgaccggatc gccattggcg gcttccgcca ccagcgtggc gccatcgaag cgcacggtca
    52141 tgccaccctg gcacgaggcg aaggtgggat cggcggcttc gatcttgccg tcctcgcgga
    52201 tgacacgcac ccgttccaga ttgttggaga aggtcagggt gccgcccgtg accccggcca
    52261 gcgccgagcc gccgcgacgg atgaagccgc ggccctggct gaagcgcttg agcgcaaaac
    52321 tgtccggcgt ggcgtcgatc gtcgccgccg caccttcctc gccctgcgcc accagctgca
    52381 ggcgcgcatt ggccggacct tcctggccca tctcgaaggt gaggttctcg acgaccgtcc
    52441 ccaggtggcg aaagaagacc ggggtcacca gctttggatg gccaacctcg aaggtgaagc
    52501 tcggaatgtc gtcggcgccg ctgtcccaga catgcgcgta accgccgccc ttgagcgtcg
    52561 ttcccgagac ggtgccgttg ctcgtcgccg atgctgcgag cgtgaaggca ttgccggtgg
    52621 ggcccgccgt gtcgtgcgtg atcgtgagcg tcgtgccgcc actcgtgcca taggtcgcgg
    52681 cggacacaag cggcgaggcg ctcgcgttga gggcagcggc ggcattggtc agggttgcgg
    52741 cgagcgtgcc gccgatttcg atctggttgc ccgaaggcgt acccgagacg aaggtccaga
    52801 ccacgccatt gatcgtgatc gtgctggtgg ccgtcggctg cccggcaaag gcgatgctgc
    52861 ccgaagccgc gacggtcgtc gtggtcggat cgccgaacag ggcggtcagc cagaacccgg
    52921 tgccgcgcaa gtcgagcggg atttcgatct gcccttcatc ggtgatcagc ccgcgatagg
    52981 gatcctgcgc gttgcgcccg cgcccgagca gcggatcatc gcccaatggc tggttcgagg
    53041 agagatccgt cgatttgaaa tccagactgc gatagccggc gagcggcgcc acaccgtagc
    53101 tgacctctcg ggcaaccttg agcgtggcgt ccgcgccgta agcgcgcacc ttggccatat
    53161 cggactcctt gggtagcggt gttgatcagg aggtgagcgg atcgctcacc aggtattcga
    53221 tcgtgatggt gaggcgggcg ctcaggatcg gcgcggcgcc ttcgatcgcg agtgccgaca
    53281 cctctggcgc ggaggtattg aggttctccg caaggccgcc catcgatggg tcggttgcca
    53341 gcgccaggcc gatgtcgcca agcaggtcat cgagggcggc ctcgccgctg ccatcggccg
    53401 cacgtgtcac ataggtttcg atctcgatcc ggtggctata gaactcggtg cgcggattga
    53461 gcgtgacgtc gggctctccc ggctcgccgt cgcgcaggat caccaggccc gccaccggca
    53521 ctttctcggg cagggtctcg tttcgccgca cggtggccga aagcagggtc tggagccgtt
    53581 cgaacagggc ggacagaatc gcctcgcgtc gcgacatcag cgcgtgctcc cttccgtcaa
    53641 tcgccactgc cgcaatacaa gttccggcaa tcggccatgc cagcgccggg cggcgcccgc
    53701 aaaatcgagc cgtttcttga acgtcacgct cggcaccagc acgaagatcg gcaccgtgct
    53761 gcgcccggag agccgggtga aggtcgaacc ctgccggcgc ccctgattgg cgacggcgcg
    53821 ccctcggctg ttcagccgcg aattgtcggc gaccagcaag gatggcgcgc cacgacggta
    53881 gacgaagcgc aggcgcatcc ccgttcgccg ctcccagccg cctggcgtga tcttggcgcc
    53941 gccatcgccg tagcgtcccg ccgccagcgt cgggatcgcc aggaacagcc ccgtggtgga
    54001 gcggatggtc gcgccgtcct cgtagatgcg cacgattccc ggcgccttgc tccagacgaa
    54061 gcccgcggcg gcgacgctgc tctggccttt tggatagacc tcgccgcgcc aggtccgggc
    54121 gagtttctcg ccaaggccgg ctccggtgat ttgcgttctg agctcgcctt tgagcccatc
    54181 cgtcgcttcg cgtaccccca gcgtgatggc gcgctccgcc cctttgagct cggcctccat
    54241 cagctgcttc aggctgcccg acagggcggc ggagagtctc atggggaacg tccctcgacc
    54301 gtccagatca gccgttccgc gtcacgcacc ggctcgccct gaacgagcag gatgtcgcca
    54361 tcgatctcga tcgtatcgcc ggcggcaatg gtcggcgctt cggagacgcg cagatcgacg
    54421 agagcgaccg gcgtcacgag gcgcgtgtcc ccgaacgccg agatcgcctc gggacgtcgg
    54481 acgacggcgc ggatcgccac gccggatccg gcgcctgcca cccgccagat cgcatcg
//
LOCUS       DAORNH010000003        50152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_513_length_50152_cov_2.582310, whole genome shotgun sequence.
ACCESSION   DAORNH010000003 DAORNH010000000
VERSION     DAORNH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 50152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50152
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_513_length_50152_cov_2.582310"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1421)
                     /locus_tag="PK264_00515"
     CDS             complement(147..1421)
                     /locus_tag="PK264_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRD74412.1"
                     /translation="MDCTESTRLSRRHLLKGGAASLALWGFMPRTALGAARDPRLLTI
                     VLRGGLDGIAMAAPVGDPAYASLRGAIAVPASGTGAGLPLDSLFALNLNMPFLHGLYL
                     KREALIVHAVATPYRGRSHFDGQDVLESGLPGVVRADDGWLNRALAGLPKGDKVAPRK
                     GLSMGAVVPLVIRGGAPVLSWVPKTYNLPLREQTIARLLDLYNVTDPRLSKALTEGLE
                     IDRVATASAAAPAVSPAGSGAMASVAAAPASGARPFAEFIGAAEAAARFLSTADGPRI
                     GALSYNGWDTHANEGVINGLLANRLAGLDAAIKTFAEGMGAAWKETVVVIVTEFGRTA
                     RVNGTAGTDHGTATAAVLAGGAVKGGRVVSDWPGLAEKALYEGRDLAPTRDLRGVLKG
                     VLSDHLGIPEGALAKAVFPESGAVKPEAGLIG"
     gene            complement(1426..2883)
                     /locus_tag="PK264_00520"
     CDS             complement(1426..2883)
                     /locus_tag="PK264_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HRD74413.1"
                     /translation="MAKQDTATALRRFGLGARPGDHARIAADPRGYVLAALADRQAAL
                     LDDPDLDPSHVIFTDVLTAQSAIRTARLEAKGVVGSDKAKGAPPAGPAAPAGSSAAGA
                     PAPPKLAPNPLGRLGRLRNLALQDEIAARLERAITSDQPFIERLVMFWSAHFCVSAAK
                     GNVRALAGAFEREAIRPHVLGRFADMLKAVEQHPAMLIYLDNQFSIGPNSIAGLRVKR
                     GLNENLAREILELHTLGVDGGYSQVDVTNLARILTGWTFGGPQTQIGETGKFVFVANR
                     HEPGNWTVLGKTYLDQGVMTGEAVLEDIARQPATARHIALKLARHFVSEAPPQALVAE
                     LAETFLKTGGDLAALARTLVAHPAAWSTPAVKIVPPYDFLVSLARGLGHRPKAGEFMR
                     LSAALGQPIWMPPSPKGWPEDDNAWTGPSAIRERLRIAEMAARQIDKLADPRTVAADA
                     LGPRLSDETRQAIARAENREQGFELLIMSPEFQRR"
     gene            complement(3027..3737)
                     /locus_tag="PK264_00525"
     CDS             complement(3027..3737)
                     /locus_tag="PK264_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD74414.1"
                     /translation="MLPTSCRTCAIRDAALCRALPDGALAQLNQIARRRRYHAGAQIF
                     SGHEDRQLVASIVHGVVRHSKALSDGRTQIVALQFASDFLGLPTSPQNAVLTEAATDV
                     ELCTFTRAQLDVLVRAHPAVQTQLMQRLAKDLEDARDWMVLLGRKTAEERVASFIMLC
                     LAKHRKLACGATGTSAGERIELPLSRTDIADYLGITLETVARKLRQLSDDGVIAINTG
                     RFLTVLDTGMLQARAEPA"
     gene            3945..5591
                     /gene="ctaD"
                     /locus_tag="PK264_00530"
     CDS             3945..5591
                     /gene="ctaD"
                     /locus_tag="PK264_00530"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HRD74415.1"
                     /translation="MAVAEHTHAHDHPSGWRRWLLSTNHKDIGTMYLIFAMFGGIVGG
                     ALSVGIRLELQEPGLQYFGSTHMFNVFTTGHGLIMVFFMIMPSLFGGFGNWFAPLMIG
                     APDMAFPRMNNISFWLLPSALTLLVISLFVPGAGPCVVSLDAATRDLSGCGAGIGWTA
                     YAPLSTKGHSGPAVDFAILSLHVAGASSILSSINFITTIFNMRAPGMTLHKMPLFVWS
                     ILITAFLLLLSLPVLAGAITMLLTDRNFGTTFFTSSGGGDPLLFQHLFWFFGHPEVYI
                     IILPGFGLVSHVIATFSRKPIFGYLGMAYAMVAIGVVGFVVWAHHMYTSGINVNTQAY
                     FVFATMVIAVPTGIKIFSWIATMWGGSIVFTTPMLFALGFVFLFTLGGVTGVMLANAG
                     VDRALHDTYYVVAHFHYTMSLGALFTLFAGWYYWFPKMSGFMYSEPLGKLHFWLTFLG
                     VNLTFFPQHFLGLAGMPRRYVDYPDALAGFNKISSLGSYMSALGAIVFFIGIAAAFRA
                     RRPAEANPWGKGATTLEWTLSSPPPFHSFETLPRIKDDDH"
     gene            5588..6136
                     /locus_tag="PK264_00535"
     CDS             5588..6136
                     /locus_tag="PK264_00535"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRD74416.1"
                     /translation="MTDEATASRRADAILCTFDLWPHRSLGTSGMAWLFLALSTGLGI
                     GVLRAPTRVMWPMIAGSLLTLVIMAVALWRNQRSARYGERIEIGPGILRLTRFVHGGR
                     HDGPEFSTHWVRIEIAQHRHVERRIELVEGGRRAAIGDFLSPEERDALAAALTDAIRT
                     ARSAAPRVAGSMPADHRQPQCT"
     gene            6219..6626
                     /gene="queF"
                     /locus_tag="PK264_00540"
     CDS             6219..6626
                     /gene="queF"
                     /locus_tag="PK264_00540"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRD74417.1"
                     /translation="MAGKAAVLERVPNPHPDTRYLARFTAPEFTSLCPVTGQPDFAHL
                     VIDYVPDQWLVESKSLKLYLAAFRSHGAFHEDCTVAIGKRLVALLAPQWLRIGGYWYP
                     RGGMPIDVFWQTGVPPAGLWLPDQGMPAYRGRG"
     gene            6724..8214
                     /gene="gatA"
                     /locus_tag="PK264_00545"
     CDS             6724..8214
                     /gene="gatA"
                     /locus_tag="PK264_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRD74418.1"
                     /translation="MTDLTRLTLTEARDGLRAKKFSATELTKAFLGAIEKANGALNAY
                     VLPTPEHALAQAKVSDQRLADGSARPLEGLPLGHKDLYCTKGIRTTACSKILGDFKPT
                     YESTVGQNLWDAGAIMLGKLNNDEFAMGSSNETSCFGGVTNPWQRVRNGAIDNARLVP
                     GGSSGGSSAAVSADLCLAATATDTGGSIRQPAALTGTVGIKPTYGRCSRWGIVAFASS
                     LDQAGPIAKTVRDAAAMLRQMASHDPKDSTSVNAPVQDYEAAVGQSVKGLRVGVPKEY
                     RVDGMSKEAEDLWAKGIAWLKDAGASVHDISLRHTKYALPAYYIVAPAECSSNLARYD
                     GMRYGLRRPGKDLIDTYEATRAEGFGAEVRRRVMIGTYVLSAGYYDAYYLKAQKVRTL
                     IKRDFDEAWAKVDVVLTPTTPNPAFAAGEKSGDPIAMYLEDIFTVTVNMAGLPGISVP
                     AGLSAEKTPMGLQLIGKPFDEATLFRAGAVIEKAAGRFTVPSRWWV"
     gene            complement(8371..9357)
                     /locus_tag="PK264_00550"
     CDS             complement(8371..9357)
                     /locus_tag="PK264_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004123747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD74419.1"
                     /translation="MPTPIAVDGLDIQIIVDNVTDNLSSTPAFVETETAALSRRRRGA
                     WVLDGDCLCCAAHGLSCLVTLRRGDETRTVLFDSGPEDRVFEQNVSRLGIDIAPVEAI
                     VLSHGHWDHAGGMLRALQMIRDRNGGKTVAYYAHPEMFRSRAARLADGAIRPMKDVPS
                     IAALEANGASVVSSREPQALIDGLAVVSGEIARVTPYETGLPGQVRRTLDGTGWEPDE
                     LLMDERSLAFNVAGKGLVVLSACSHAGIVNVLTHARKMMPDVPLYAALGGLHLSGTNE
                     RIIPETVEALRGFGLTVLGAGHCTGWRAMTALANAFGDKVLAPLAVGKRFRF"
     gene            9738..10319
                     /locus_tag="PK264_00555"
     CDS             9738..10319
                     /locus_tag="PK264_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74420.1"
                     /translation="METLTKIDVARRQLVTAIRLLFDDRDPVSVYSLATNAQEILSTL
                     CERRGVLSLRGNIAAPTGMNSDRIQTQLINPARNFFKHADRDPDSVWRDFTDTDCDHI
                     LLIPSIDFVMLERKSPIEAQVFVTWYAALYPDKIPQNTDWSDAAARHFSGIRAAHRSD
                     QKRAASAIIATALKNPALMAHPETVPSEVRRWS"
     gene            complement(10255..10995)
                     /locus_tag="PK264_00560"
     CDS             complement(10255..10995)
                     /locus_tag="PK264_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74421.1"
                     /translation="MTERRKEVDPNRVQQLLLCLDGLRYSFAIILSCHARVRSALNSF
                     EADWQSGASSSAVMQVVADTWMMIDATQRIRLLIERTPTLSKNEPAIRVFKDGTSAVE
                     PLRHYVQHINNEVTRITGQAQPLWGSISWVSAADRLTQFSLCTGTLHLRASVPGLVFD
                     RQEMRFVRSLELSVGSHSIDLDNLAQRARGLDQMIADWAGTIEFADGQKYDYRPPVAP
                     VVMIAVRTMTTAEPHSEQSQGAPSGQDS"
     gene            complement(11244..11699)
                     /gene="moaE"
                     /locus_tag="PK264_00565"
     CDS             complement(11244..11699)
                     /gene="moaE"
                     /locus_tag="PK264_00565"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HRD74422.1"
                     /translation="MIRVGEDDFDVGAEVARLTAGRTDLGAIVTFTGVVRGTVKGRPL
                     ASMTLEHYPGMTESELARIEADARRRWPIEASLIVHRIGALKPGDNIVLVVTASAHRQ
                     AAFEAAEFLMDYLKTRAPFWKKETFLDGSGDWVDARESDGAAEARWERG"
     gene            complement(11704..12372)
                     /locus_tag="PK264_00570"
     CDS             complement(11704..12372)
                     /locus_tag="PK264_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD74423.1"
                     /translation="MPELIINGPAGRIEARYHHDGRPRAPVALILHPHPEFGGTMNNP
                     IVYSLYYAFVNRGFSVLRFNFRGVGRSQGYFDNGPGELADAASALDWLQLQNQDAGGT
                     WIAGVSFGTWIAMQLLMRRPEVDGFVCVGPPANLYDFTFLAPCPSSGILVNGDKDRVV
                     PTAAVAELAAKTKTQKGIKITHEVVPGANHFFESKTEELAEVVGAYVDQRLKAKREER
                     AKGE"
     gene            12767..13252
                     /locus_tag="PK264_00575"
     CDS             12767..13252
                     /locus_tag="PK264_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRD74424.1"
                     /translation="MELNTKGRYAVTALADLARHAGEEAVPLSVIATRQGLSLAYLEQ
                     LFLKLRRAGLVESARGRTGGYKLCRPAESISVAEIMLAVEEGVRMTRCRGDDSAPCQA
                     GERCLTHDLWDALGAEIARFLDRVTLAEVIDGSLGERRRASRASGNAASTGPRGAPAN
                     G"
     gene            13245..14444
                     /locus_tag="PK264_00580"
     CDS             13245..14444
                     /locus_tag="PK264_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRD74425.1"
                     /translation="MADARAYLDWNASAPLRPEARSAMLAALDFCGNPSSVHAEGRAA
                     RAIVETSREQVAALVNARPSEVVFTSGASEANATVLAAPCRHVIASRLEHPSIAVPAA
                     QSADFVELRAGSEGIVAAEAIADLVLRTTRIAPELSLVTLQLANGETGVVQPVAELGA
                     FCHGHGIHLHCDATQAAGRMVVDCEALSVSTLALSAHKIGGPKGVGALVIREGCTVPA
                     LITGGGQERRRRSGTENVAGIAGFGAAAAAARADLADGASARVEIVRDRIERGLRAIT
                     PDLVVIGEGAPRLANTSCVALAGSRSETLVIRLDLAGVAISAGAACSSGKVGRSATLE
                     AMGLSPEVAGSAIRISIGAATSDREIDLLMHAWSAIHATSGVEARPRRMHADLSPEPR
                     PFAVGEH"
     gene            14448..>15461
                     /locus_tag="PK264_00585"
     CDS             14448..>15461
                     /locus_tag="PK264_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006012385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRD74426.1"
                     /translation="MAAVQQTIDQVKALDVDKYKYGFETKIESVLAPKGLNADIVRYI
                     SEKKGEPEWMLAWRLEAFRRWQAMTEPTWAKVSYPKIDFQDLHYYAAPRSTEGPKSLD
                     EVDPEILKTYAKLGIPLKEQELLAGVRKPGEPSHIDDAEGFASMAGKVAVDAVFDSVS
                     VVTTFKKELAKAGVIFCSISEALREHPELVRKYLGSVVPTTDNYYATLNSAVFSDGSF
                     VYVPKGVRCPMELSTYFRINEKKTGQFERTLIIADKGSYVSYLEGCTAPMRDEHQLHA
                     AVVELVALDDAEIKYSTVQNWYPGDKDGKGGIFNFVTKRGDCRGRNSKISWTQVETGS
                     AITW"
     assembly_gap    15462..15493
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15494..15954
                     /locus_tag="PK264_00590"
     CDS             <15494..15954
                     /locus_tag="PK264_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006012385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRD74427.1"
                     /translation="YSIAVSNGRQQVDSGTKMIHLGRNTSSRIISKGIAAGHSQNTYR
                     GLVSAHRRATGARNFTNCDSLLIGGKCGAHTVPYIEAKNASAHFEHEATTSKISDDTL
                     FYCMQRGLGEEEAVALVVNGFVRDVLQQLPMEFMAETQKLIGISLEGSVG"
     gene            16225..16518
                     /locus_tag="PK264_00595"
     CDS             16225..16518
                     /locus_tag="PK264_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74428.1"
                     /translation="MTDAAGMGELIATAKLVRQIGEDECRRRNALSRTVPGFTLKPGQ
                     IGNVLEKFGEGEAFLVEFGPKANSCDWLGVLYSSELELVSSADIPAARIVRAA"
     gene            17118..17873
                     /gene="sufC"
                     /locus_tag="PK264_00600"
     CDS             17118..17873
                     /gene="sufC"
                     /locus_tag="PK264_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRD74429.1"
                     /translation="MLEIKDLHVRIAEEDVEILKGVTLTVPKGEVHAIMGPNGSGKST
                     LAYVLAGREDYEIVSGDILWQGESLIGMAPDERAARGVFLAFQYPFELPGVATMTFLR
                     TAVNAVRKKRGTKELTTPEFMKLVKARAAELGISDDMLKRPLNVGFSGGEKKRMEVLQ
                     MLMLEPSLAVLDETDSGLDIDAIRIVSEGANKLRSKDRAQLVITHYQRLLNYIVPDRV
                     HVLAEGRIQKSGGKELALELEKSGYAGFRSKAA"
     gene            18020..19369
                     /gene="sufD"
                     /locus_tag="PK264_00605"
     CDS             18020..19369
                     /gene="sufD"
                     /locus_tag="PK264_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HRD74430.1"
                     /translation="MTTTEKTMAEMALAAEFDRIIDRLPGEGLRDERRAAMARLAASG
                     LPHRRIEEWKYTDLRAGLKEVFAAADGANGVTAADVAAALGPLGTLEAHRLVFVDGIY
                     APALSPLDALGTDAIAVKVAAASQTFVPTAKPISEGARGDALQALNEAFASDGAIISV
                     QDDASTSARDAKVRSGSPAKPIMIVSLSTARAPLRVTTRHRIEIDAGMTATVIEVYGR
                     IGSSAIQTSAGSIVRIGKGAKLHHHKFALEGSRGAHLSSSDVEIGAGATYRAFHLTPG
                     SGLARTNVFATFAGPDARLDISGAFLGAGADHVDTTLVIDHAVPGCESRELFKGILAG
                     DAKGVFQGKVIVRPDAQKTDGKQMAQALMLSPTAEFDSKPELEIYADDVICGHGSTTA
                     ELDAELMFYCRSRGIPEAEARTLLIDSFIGEALAKVEDGELREACLEIVAGWLKTNL"
     gene            19426..20691
                     /locus_tag="PK264_00610"
     CDS             19426..20691
                     /locus_tag="PK264_00610"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRD74431.1"
                     /translation="MSTPATPDRAIRTGGSGAFDVERIRADFPILFREVYGKPLVYLD
                     NAASAQKPRSVLDAMDHAYKFEYANVHRGLHYLSNTATSRFEEARAITRRFLNARYEE
                     EIVFTGNATDAINLVASSVGLSLAEGDEIIVSIMEHHSNIVPWHFLRERKGCVLKWAQ
                     VSDSGEFLLDEFARLITPKTKIVALTQMSNVLGTVTPVKEIVRLAHEKGVRVLIDGSQ
                     AAVHMPVDVQDLDCDFYVFTGHKVYGPSGIGVLYGKKAILDSLPPYRGGGEMIEAVHV
                     DKITYGKSPHRFEAGTPAIVPAIGLGAALNYMMQVGRDRIAAHEADLGAYAHARLGEL
                     NWLKIHGRAKGKGAIVAFSIDGMHPHDISTIIDRSGVAVRAGHHCAQPLMERLGVTAT
                     CRASFAMYNTRAEVDALANALIKAHDFFA"
     gene            20938..21234
                     /locus_tag="PK264_00615"
     CDS             20938..21234
                     /locus_tag="PK264_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HRD74432.1"
                     /translation="MTADIVAALKTVYDPEIPSDIYELGLIYKIDIGDNRDVAVEMTL
                     TAPGCPVAGEMPKWVENAVNTVPGVNNTTVTLTFDPPWDQSRMSDEARLALNMF"
     gene            21287..21559
                     /locus_tag="PK264_00620"
     CDS             21287..21559
                     /locus_tag="PK264_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74433.1"
                     /translation="MPETSRAEARIVEEVALARSIVTYHGDAAKTAAMDHLYSRFVKP
                     GDLAFDIGAHVGDRTASFLRLGARVVALEPQGGPAAVLACSATTSA"
     gene            21556..>21923
                     /locus_tag="PK264_00625"
     CDS             21556..>21923
                     /locus_tag="PK264_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRD74434.1"
                     /translation="MTLINAACAATVGRVTFRINSANPTVSTASREFVSAADGAGGWE
                     GQVWDREVEVPATTLDELIARHGTPAFTKIDVEGFEASVLAGLTRALPALSFEFTTIQ
                     RDVALACLNRLAALGAYRFD"
     assembly_gap    21924..21933
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22148..23425
                     /gene="hisD"
                     /locus_tag="PK264_00630"
     CDS             22148..23425
                     /gene="hisD"
                     /locus_tag="PK264_00630"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRD74435.1"
                     /translation="MAREYLKKAAVSKAMRKAEVEETVRRMLADIEVNRDDAVRKYAR
                     ELDKWTADFRVTKAEVEAKSKSLSKVFKEDFALCHKNVTEFAKRQKDSMRPFESELTP
                     GIMLGQKLIPVSSVGCYIPGGKYPLISAAIMSVGTAKVAGVGRIVACAPPRGPDGIYP
                     QTLYALHYSGADEIYCLGGVQAMASMAHGCVGMPKADMITGPGNAFVAEAKRQLFGVC
                     GIDLLAGPTEVAVIADEGADPALVATDLLGQAEHGPDSPAWLITTSRALGEAVANEIA
                     KQLPSLPTRANAEAAWRDHGEVVVVDSDDEAIAVSDEYAPEHLELQTRRDDYYLERLK
                     NYGSLFVGEESTVAYGDKGVGTNHTLPTEGAGRYTGGLWVGKFIKTVTYQRLTRRASR
                     EIAPAMSRICREEGMIAHAITGDVRVKRFQGAN"
     gene            24178..25029
                     /locus_tag="PK264_00635"
     CDS             24178..25029
                     /locus_tag="PK264_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74436.1"
                     /translation="MPSLINKFDLSSSVRYIKNVGQHKALMEHPLLLASPLARSVLVI
                     EIPAVPFGAHAAMIIIDPSSTMNSQLINDLHEGAALASELFHRADNAVPSSLRPPGLA
                     EGVPEGAVTEFLLETLKTTRSLKSRRKTSYLTLRTWQTSIKKFQISAIKSLKKQPSPE
                     FVSAIASEFINASQMLVGTKGIDTVVPMPCGHSRSDECLSVRIARRIAEAYPLTFHMA
                     FKSVHRSGSSHPRRNVDLPAFKLVSPPSGNVLLVDDIATTGTHIEKAKEILLKQARNV
                     LAIAWIG"
     gene            complement(25079..25702)
                     /locus_tag="PK264_00640"
     CDS             complement(25079..25702)
                     /locus_tag="PK264_00640"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRD74437.1"
                     /translation="MNNSLVLVRHGESAWNKLNLFTGWKDPDLTEKGVAEATAAGRKL
                     KDLGLRFDVAYTSALLRAQHTLALILRELGQSDLPTHRDQALNERDYGDLSGLNKDDA
                     RKRWGEEQVHIWRRSFDIPPPGGESLKDTAERVLPYYMARIWPDVKDGKSVIVAAHGN
                     SLRALIMHLEGLSGEAIIKRELATGAPIIYRLTVEGRVADRRDIPVR"
     gene            25882..27123
                     /locus_tag="PK264_00645"
     CDS             25882..27123
                     /locus_tag="PK264_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD74438.1"
                     /translation="MTRERKSFLFLNVGHFLDHLFTLIFASVAALALVKQWGISYAEL
                     TLYGLPGFVAFGAFSLPAGWLADRWSRHGMIVVFFIGIGAASILASFARSPLELSLAV
                     FVVGMFAAIYHPVGLAMVIEQAKANGTGMMLGINGIWGNLGVGSAALITGAVIDWLGW
                     RWAFVIPGVLSILVGLGYWQHARDEIKAANARVSPKVTIASAKAAASMPSDHRRALIR
                     LTAIIFFTTAISSIIFQSTTYALPKVFEERLPELAGSATKVGWFAFVVFAIASVAQLM
                     VGKALDVYGPRLVFMVVAAVQVTFFSLMPGLRDLPALLVALGFMLGAFGQIPINDYMI
                     GRMARSELRATIYGVRYFVSFIALAATLLLVAWVHKNWGFDVLFRILAGTAALIFLAV
                     SLLPTRLPDADAAPLPATAKA"
     gene            complement(27160..28107)
                     /locus_tag="PK264_00650"
     CDS             complement(27160..28107)
                     /locus_tag="PK264_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRD74439.1"
                     /translation="MITTRRAVNGAALATLASGLIGPARAQAPIVIGHSQPNLGWPYI
                     AAVTRLLEAEAKAKRVTLVTLSANGDIAKQSSDIAQLVNRKVKVLLVTSLDGNAVVPA
                     LKRAKDVGIPILAVSNEPSEAGQAMLAGYSGPDDYVQGRLAAELLHEALAGKGGIGII
                     EGSPGQSTTLLRTKGLKDRLAELKSEIAVIGAQTADWNPVKAKDVAQAFLTAHGAKLV
                     GLFAQDDNSGAAAAEVFKAVGRSDVKVVGTGGTKNGLAAIRSGLMYGTVDQSPAADAR
                     QALDIALALAAGQMPKEKRSLIPMPKITAANVAGFTGEW"
     gene            complement(28132..>28426)
                     /locus_tag="PK264_00655"
     CDS             complement(28132..>28426)
                     /locus_tag="PK264_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD74440.1"
                     /translation="AGLAGLFLAARLGTGSPIIGDATPLTAAAAALIGGATLKGGEGS
                     ILGAAAGLLFIATLVNVLNHLGIPAYYQHMAIGGMLIALVVTDGMLARASTAK"
     assembly_gap    28427..28530
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28531..29066)
                     /locus_tag="PK264_00660"
     CDS             complement(<28531..29066)
                     /locus_tag="PK264_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74441.1"
                     /translation="MSLLVRPWLRMLFIKPPLVPLLALAWFIDGFWTIGNIRVLLESV
                     AVDGFIVAGMTIVMIAAGFDLSVGAVMALGGVASVVLLPYGLLVSFAGAIGLGALAGV
                     TSGTFVTRLHINPFIATLAVMVVLRGALLAYTGTRPVVGLDERFLALGGGDPIPYAFL
                     LMVLVLTLLHLSLSSRPWG"
     gene            complement(29063..>29400)
                     /locus_tag="PK264_00665"
     CDS             complement(29063..>29400)
                     /locus_tag="PK264_00665"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HRD74442.1"
                     /translation="VVGVGITTFVISGTLAALAGVIIAAMVGVIDPTAIARYELPALT
                     AAVLGGTSLAGGVGRPADTAAAILIVSIIANVMTILNYQHASQLMVQGIVLVAAVAFY
                     ARSRGEGAT"
     assembly_gap    29401..29423
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29424..30043)
                     /locus_tag="PK264_00670"
     CDS             complement(<29424..30043)
                     /locus_tag="PK264_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD74443.1"
                     /translation="MSSASETAPLLRATATRLAGVAPIWLVLVAVIAAAALVSDRFLT
                     SANIVAVLQQGVITGLVALGMTIVLIGGEFDLSTGSTVMMAAVVALLMGPSTPAGVML
                     AVLVPLLIGATVGLLNGIAVSRLGANSIVATVGMQFLLLGAVLALVGGQHVRAESIHP
                     VFTGLALWRPLGIPLPVMLFAVLVAGFAVMMSSTVLGRHIYAVGGD"
     gene            complement(30040..30810)
                     /locus_tag="PK264_00675"
     CDS             complement(30040..30810)
                     /locus_tag="PK264_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74444.1"
                     /translation="MGPDPLVRLTGITKSFGGVRALRGVDLAVAAGEVVALVGHNGAG
                     KSVLVQIVSGVFAPSSGRLEVAGREIALASPNDARRHGIETIFQTLALADNLDGPANF
                     FLGRELTTRLGFLDKRRMAGEARQALLSLNPQMTDIERPVSQMSGGQRQAIAIARAIH
                     FDVKLLVMDEPTAALGPHETAEVGALIARLRTKGIGILLISHDIRDVMRLADRIVVMK
                     SGEIVGTLDVKATSEDEVVDLIIRGRREGSGIRPEAAR"
     gene            31355..32650
                     /locus_tag="PK264_00680"
     CDS             31355..32650
                     /locus_tag="PK264_00680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HRD74445.1"
                     /translation="MAEAQMIPEVRDTEIERLLREYAQPILRAAGQEKGRIRVRIVRS
                     EVFNAFVIDGRSIYVHTGLLVQAKSPNQVIGVLAHEVGHIANGHLAALRDRIQRDQTK
                     SLLIKLLGIGLLIAGGTSGSAGTSSSGSAVLSGGDEILLRSLLAERRSQESSADQSGL
                     KYLFATRQSGRGMLETFEFFAQQEYVSDQYKDPFVRSHPVAAERLARLRDAVMASPFY
                     GVTDPPALQLRHDLMRAKISGYLERMQIVFNKYPRTDTSLPARYARAIATFLGSGLDP
                     ALPLVDELIREKPDNPYFLEVKGDFYARSGKAALAVEPLRRSLKLAGDAPLIRTRLAE
                     ALLALEDPRFYPEILDLMRKTIIEDPDPRAYRAFALVNYKLGRRPQAELATAEAYFLE
                     GDFKQAQTFARRAQAGLRNGESDWLRANEIVTFKAVAGQ"
     gene            32704..33603
                     /locus_tag="PK264_00685"
     CDS             32704..33603
                     /locus_tag="PK264_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD74446.1"
                     /translation="MSSPGFGSFFDMLTSKKGAALAVPAILLFGATLAIGQKSFDAMA
                     QDKAAPPAASAAAPATPGAPAAPAGTSSAGVDRKEVEKIIREYLIANPEVMIEVQTAL
                     EAKMEKIKAEKMKTMLGQVAGRLYRSPTAAVAGNPSGDVTIVEFFDYNCGYCKVSLAA
                     VAKVIEGDPKVRVVFKEVTFIGRETSAGASRIALAARKQGKYWEVHRALLENKGVVNE
                     ALALKVAEKFGLNMDQLKRDAASAEIKKELDDATALAESLGIDGTPFFLVGDRVISGA
                     GESLANSLQSLVADVRKTGCKVC"
     gene            33866..34366
                     /gene="accB"
                     /locus_tag="PK264_00690"
     CDS             33866..34366
                     /gene="accB"
                     /locus_tag="PK264_00690"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRD74447.1"
                     /translation="MPKDSKDKDAADKGLIRELAELLVETGLSEIEVEREGLKIRVAR
                     SLTVAATVPMPMMPAPLPGTAASSAPRGDALPAAGAPAAADPTSHPGCVKSPMVGTAY
                     RAPEPGASPFVDVGTRVNQGQTLLIIEAMKTMNHIPAPRSGTVREVFIENGQPVEYGE
                     ALLIIE"
     gene            34440..35783
                     /gene="accC"
                     /locus_tag="PK264_00695"
     CDS             34440..35783
                     /gene="accC"
                     /locus_tag="PK264_00695"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRD74448.1"
                     /translation="MFDKVLIANRGEIALRVQRACKELGIKTVAVHSTADAEAMHVRL
                     ADESVCIGPPQAAESYLNIPRLLAACEITGADAIHPGYGFLSENARFAEILEEHNLTF
                     IGPTAAHIRTMGDKIEAKVTAKRLGIPCVPGSDGAITNEMQAQRVAREMGFPVLVKAA
                     AGGGGRGMKVARSAEELDLAIKTARAEAKAAFGDDSVYLEKYLTHPRHIEVQVFGDGK
                     GNAIHLGERDCSLQRRHQKVWEEAPSPALNSENRKKIGQIVSKAMQKLAYRGAGTIEF
                     LYEDGEFYFIEMNTRLQVEHPVTEMITGLDLVLEQIRVASGGTLSVTQDEVTFSGHAI
                     ECRICAENPRTFRPSPGKITYWHPPGGLGVRVDSGVYQGYKIPPYYDSLIGKLIVHGK
                     NRNECLMRLKRALSEFVIDGIETTIPLFMDLIQHPDIQNGLYDIHWLERYLADKA"
     gene            complement(35786..40369)
                     /locus_tag="PK264_00700"
     CDS             complement(35786..40369)
                     /locus_tag="PK264_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD74449.1"
                     /translation="MFKLLRYFSLSSAVIIVAATAVLVLLFKWQQTRTILLTTERNSA
                     ELTKAIANSVWGSHRDFLTKARRAHSANASQGPEITALDRDVLQAISGIDVLKIKIYA
                     ADGVTIYSTEHGQIGEDRSTDANHVAVQKTLLPISRFSFRKSFNGVRGVVRNVEVAET
                     YVPVVENGKLVTVFEVYKDISTEIQQLKRNTLFVGLFAVAAFSLLYLVLFAVVRRADL
                     IIEDQYGELTKLNSSLEQRVAERTAALREQEEAARATSERLAREIEQKKAAEGELIRK
                     REDLLMQQLVLNELIRSNSVSRGDWRGAIQHLTNASAVTLGVTGASAWIVDQDGSVLR
                     RIEGEQPPEQASVWPNELTREANPKLLAAIESGEVLTVEDVRTNPATRELVEVWLGPG
                     HVVSLLGVPIIRDGRPIGVVFFESENVKAEWTPDRQVFAASIANLVASVIERRDREML
                     QTESARRTLALVNQQATLNELVRGKALTSAPLLTAFDTLTKAAARALEVDSVTIRMFV
                     KDRQGVHCVSRYRAAERAHSRGDDVPIADLASAVAAIENGQPLIIESAATHPATRKFY
                     QSQLKPFGIAAIIFVPIMRNGGLAGYISYGRSDERWPWTTEQQVFASSIASLAHMVLE
                     RTDREALEIENSSRTDGLLAQQATIGALMRDPAIRFGDIRTAIETIAKSMTLNMGTDR
                     GGVWLLDDTRTRFAASSVYDAVRGEFVAPKYSENTEFAALMIQASAHRIVTIDDTSEN
                     HELAHLQSLVFDAIGATSVMHVPIMANGDIAGFITTASIGRRLAWTADHRLFGAGIAN
                     LTALVVERGARERIAEQLVDANRKLEGATRAKSEFLANMSHEIRTPMNGVFGMTDLLM
                     RTELNDRQRRLVGTISQSARTLLTIINDILDLSRIEAGKLELDHREFDLRYTIETAVD
                     LFAEEARRKELELSLFIADDVPTTVAGDPGRLRQICVNLIGNAIKFTNDGEVSIRLTR
                     DGGSERGTHVRLEVRDTGIGIDKAIVAKLFSPFTQADSSISRRFGGTGLGLSISLHLA
                     RLMGGKLSVDSEFGKGTIVTLDLSLQVAADAPELRPDWTQLVGRRILVADDREANREI
                     VVNYLSGQGAEVVAVASAGAAIAALEKAGRAGTPFCIALLDVVMPGVDGIELVGAIRA
                     TPEIAATKIILLTSLSWKGDVSKARELGVDALLTKPLRRGDLLDEVCRIVAGKVALPR
                     LARRESAAPRFAAHVLLAEDNPVNVEVAREFLESYGCSVDIANNGVEAVVAIEHARYD
                     LVLMDVQMPEMDGLTATARIREIERRTCLAPTPIIAMTANAYAEDREKCLGAGMNDYI
                     SKPFDEERLVSMLGIWLTRSRGARAEALSSKATSEAAIAVVSEEAPAPATGPAVAAIA
                     GPATAAITDPGPAAPAPSSPVSQPTPVVPGPAPIEEKVLEGLRTHRPELLRRLIRTYL
                     DYAPKAISDLKTANDAKDMLSLKMVAHSLKSSSANLGAQRLSALCKDLEGASNAKDEV
                     RASALAVTIVDEFAVVRARLEQMAAADALKQVS"
     assembly_gap    40604..40613
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40614..42036)
                     /locus_tag="PK264_00705"
     CDS             complement(<40614..42036)
                     /locus_tag="PK264_00705"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD74450.1"
                     /translation="MSERMDGIWSAAKPERDPAAIDRLRTAARRDLEMLNFPAPNWVP
                     TSCSADGRPVLDVLIIGGGMCGQTAAFALMRAGIRNFRIVDKAARGEEGPWGTYARME
                     ILRSPKQLTGPDLGVPSLTFRAWYESGCGEAAWHSLHKAGRIDWRDYLLFVRDTVGIP
                     VENGVEVAVIRPAGRLIEVEASSAVGQERLLARHVVLATGRDGSGGQRLPVIAGLDWQ
                     SPARRGRISHSAEEIDFASLRGKRVAVLGAGASAFDCAAAALEAGAGAVVLYCRRAQL
                     PQINKSKGASFPGFLRGVAALDDATRWKFFSYIFSEQVPPPYESVLRCDSHENFSIAF
                     GQGWRSIRLGRTEVFIDVGGEGTSVDAAILATGFDVDLTLNGLLSPHAGEIALWRDRV
                     PPDSAADHEETSRFPYLGAAFALQPAPHSKATWLGNVHIFNWGATVSQGPLSGDIPGL
                     AIGAERLASDLAARLLDADIARHW"
     gene            42058..42690
                     /locus_tag="PK264_00710"
     CDS             42058..42690
                     /locus_tag="PK264_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRD74451.1"
                     /translation="MIELDCYYSLSSPWAYFAGPRLEDIVRRHHVKLNLKPFDFQAVV
                     PSTGGVPLRTRPAPRRRYHDAELDRWRQYLGMPLNLKPRHYPEAVTDPDWNKYPGWAV
                     IAASEDGKPAMRLSQALLRALWAEERDITDPAVRRAVADENGYDGAAVVAAERSAVVQ
                     EKYRQFTDEALRLGVFGAPTYVVDGELFWGQDRLGFVDRKLDRMRADGKS"
     gene            complement(42704..43567)
                     /locus_tag="PK264_00715"
     CDS             complement(42704..43567)
                     /locus_tag="PK264_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD74452.1"
                     /translation="MSTPVLFALISAFLFALTNHFQSIGLKGADVRSGSLVNIATGAA
                     MYWLAAPFFLEGGYWWTWGFICFAVVGLFRPSLSSWLALSSIARMGPTLTSALTASAP
                     IFGAGLAILILNERLTLATTIGTLAVVAGAVVATMRPDGVARGWPLWALVLPFGAAFI
                     RAAGHVGTKLGLGDVPSPSFAVLVGNTVSLVLVAAAFFGQGQRITGRMSDHKWFVMAG
                     IAAGSSLHFLNSALAAGSVVTVVPIVSAAPVFTLLLGLLVFRNEAFSLRTAATIALVV
                     PGVVLVALSGR"
     gene            43776..44171
                     /locus_tag="PK264_00720"
     CDS             43776..44171
                     /locus_tag="PK264_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74453.1"
                     /translation="MKLKTLTLAAALVVAATGVASAQSPGRGHGPGYGYGPGHSGGDI
                     DWNQWQQKRRIEMGRSDGSLSPHEYRALIEEQRRIQDLEARFKRDGRLSADELATLRR
                     AQSAASRNIYEERHDRDTARRGWFGWGRR"
     gene            complement(44256..44873)
                     /gene="rpsD"
                     /locus_tag="PK264_00725"
     CDS             complement(44256..44873)
                     /gene="rpsD"
                     /locus_tag="PK264_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRD74454.1"
                     /translation="MTKRISAKHKIDRRLGENIWGRPKSPLNKTEKRPGQHGDRRTGK
                     LTDYGMQLRAKQKLKGYYGNIGERQFKRIYAEARRFKGSTSEHMIGLLERRLDTVVYR
                     AKFVPTVFAARQFVSHGHVTVNGKKVDISSYRLRVGDVVEVREKSRQLALVLEATKSG
                     ERDLPDYLEVDHNKMTAKMVRVPGLGDVPFPVRMEPNLVVEFYSR"
     gene            45351..46298
                     /gene="fabD"
                     /locus_tag="PK264_00730"
     CDS             45351..46298
                     /gene="fabD"
                     /locus_tag="PK264_00730"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRD74455.1"
                     /translation="MAIAFIFPGQGSQAVGMGKELAAAFPAARAVFEEVDAALGQNLS
                     KLMFEGPQDQLTLTENAQPALIAVSLAVARVLEAEKGISVAKAAKLVAGHSLGEYSAL
                     AAAGAFKLADAARLLKLRGQSMQKAVPVGVGAMSALLGVGLDVARQVAAAAAQGECCE
                     IANDNEPTQVVLSGHKTAIDRIPEAGKAFGVRRALPLPVSAPFHCALMQPAADAMAEA
                     LSKVEIKAPSVPVVANVLAAPISDPAEIRRRLVEQVTGTVRWRECVLAMSAAGVTSYH
                     ELGAGKVLAGLVKRIDKAATATSIGTPADIEAAAAGFKS"
     gene            46336..47073
                     /gene="fabG"
                     /locus_tag="PK264_00735"
     CDS             46336..47073
                     /gene="fabG"
                     /locus_tag="PK264_00735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRD74456.1"
                     /translation="MFSLSGKGALVTGATGGLGGAIARVFHKAGANVAISGTKADRLE
                     ALAAELKERVHIVPCNLADRAAVAKLAGEAEAKLGQLDILVNNAGITRDNLFMMMKDE
                     EWDQVLAVNLTSAFTLSRSVLRNMMRRRFGRIINISSVSGIIGNPGQGNYAAAKAGMI
                     GMTKSLAREIASRNITANCIAPGFITTSMTEGLNEKIVEEAKKHIPMAKFGTPDDIAA
                     AALYLASSEAQYVTGQTLHVNGGMAMV"
     gene            47367..47609
                     /locus_tag="PK264_00740"
     CDS             47367..47609
                     /locus_tag="PK264_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRD74457.1"
                     /translation="MSDIKDRVKKIVVDQLGVEAEKVTEAASFIEDLGADSLDIVELV
                     MAFEEEFGCEIPDDAAEQIQTVGDAVKFLEKSSKAA"
     gene            47771..>48301
                     /locus_tag="PK264_00745"
     CDS             47771..>48301
                     /locus_tag="PK264_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRD74458.1"
                     /translation="MTARTPQKFYAPLAVGAPAPVRQLPVRLERMIHFLPPHNEKIRA
                     RLPELVGQVDVVLANLEDAIPAEQKAAARAGAIEIARKTDFGSTGFWVRINCLNSPWA
                     LDDIMELVPAAGAKLDVVMLPKVEGPWDVHYLDQLLAQLEARHKIGKPILIHAILETA
                     EGVKNVEAIASASPRMH"
     assembly_gap    48302..48450
                     /estimated_length=149
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <48451..48790
                     /locus_tag="PK264_00750"
     CDS             <48451..48790
                     /locus_tag="PK264_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD74459.1"
                     /translation="AYGLKPFYGPFGDFSDAAACEAQFRNAFLQGCLGAWSLHPTQID
                     IAKRVFSPDVAEVLFAKKIIAAMPDGTGAAIVEGKFQDDATWKQAKVIVDLARLVAAR
                     DPAMAKAYGM"
     gene            48874..50103
                     /locus_tag="PK264_00755"
     CDS             48874..50103
                     /locus_tag="PK264_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD74460.1"
                     /translation="MAIGVTARTAFSLLLPPLIDEFKWDRGLAAGAFSFGFLVSAVLS
                     PIVGRIIDTRGPRLVIQMGVALMSTGLLLAPWVTTPWQLYATLGVAVGGGANMMTYTA
                     HSQFLPNWFQRRRGLAISIAFAGAGAGAIVLLPWLQTVILTRGWAQSCVVMGLLVLLV
                     VGPLSYLVQKAPANVGQYPDGDAAPAGSVTKRIATVVDEAWAQTEWTLAKAARTARFW
                     WIVCAYFLALIAWYAVQVHQTKYLIEIGFSPVVAAWALGAVSVVAIPGQILLGGLSDR
                     IGREWIWTAGCLGFATAYVALIALEQERSTLLLYTMIFAQGFFGYALTSVMGPIVAEV
                     FEGPHYGSIFGAITIALIGGGAAGPWIAGAVHDATGSYRLAFIGIIAGCLLSVIAIWI
                     AGPRKVRLVAGRARKSG"
BASE COUNT         8765 a        16513 c        16098 g         8448 t
ORIGIN      
        1 gtgtaaccta tgtgtccgga acgaagtgca ccccctgtct cgggaaggtc atcaacagct
       61 tggccgtcgc atcccaaatc ggggttgtca gtggccgggg cccctgacgc gggtgcaggg
      121 cggcagccct gcaatcacgc gctcaatcac ccgatcagcc cggcctcggg cttcaccgcg
      181 ccgctctccg ggaacaccgc cttcgcgagc gcgccctcgg gaatgccgag atgatcgctg
      241 agcacgccct tcagcacgcc gcgcaaatcg cgcgtcggcg cgaggtcgcg cccctcgtaa
      301 agcgccttct cggcgagccc cggccaatcc gagacgaccc gtccgccctt caccgcgccg
      361 ccggccagca ctgccgccgt cgccgtcccg tgatcggtgc ccgccgtccc gttgacgcgc
      421 gcggtgcgcc cgaactcggt gacgatgacg acgaccgtct ctttccaggc cgcgcccatg
      481 ccttcggcga acgtcttgat ggcggcatcg agcccggcca ggcggttggc gaggaggccg
      541 ttgatgacgc cttcattggc gtgggtatcc cagccgttgt agctgagcgc cccgatgcgg
      601 ggcccatcgg cggtcgagag aaagcgcgcc gccgcctcgg ccgcgccgat gaactcggcg
      661 aacgggcgcg cacccgacgc gggtgcagct gccactgacg ccattgcgcc gctgcccgcc
      721 ggcgaaacag ctggtgccgc cgccgacgca gtcgccacgc ggtcgatctc gaggccttcc
      781 gtgagggcct tagacagcct cggatcggtg acattgtaga ggtcgaggag gcgggcgatt
      841 gtctgctcgc ggagcggcag gttgtaggtc ttcggcaccc atgagagcac gggcgcccca
      901 ccgcgtatca cgaggggtac gacggcgccc atcgagaggc ccttcctcgg cgcaacctta
      961 tcgcccttcg gcagtccggc aagcgcacgg ttgagccagc cgtcgtcggc gcgcacgacg
     1021 cccgggagcc cgctctccag cacgtcctgc ccgtcgaagt gcgagcgccc gcggtagggc
     1081 gtcgccaccg catgcacgat gagcgcctcg cgcttcaggt agaggccgtg caggaagggc
     1141 atgttgagat tgagggcgaa gaggctgtcg agcggcagcc cggccccggt cccgctcgcc
     1201 ggaacggcga tggcaccgcg caggctcgcg taggccggat cgccgacagg agcggccatg
     1261 gcgataccgt cgagaccgcc gcggagtacg atggtgagca gccgcgggtc gcgcgcggcg
     1321 ccgagcgccg ttctcggcat gaagccccag agcgcaaggc tcgcggcccc gcccttgagg
     1381 agatgacggc ggctcaggcg cgtgctctcg gtgcagtcca tggcctcacc tccgctggaa
     1441 ttctggcgac atgatcagga gctcgaagcc ctgttcccgg ttctcggcgc gcgcgatggc
     1501 ctggcgcgtc tcatcgctga ggcgcgggcc cagggcgtcg gcggcaacgg tgcggggatc
     1561 ggcgagcttg tcgatctggc gggccgccat ctcggcgatt ctcagccgtt cgcggatggc
     1621 ggaaggcccc gtccaggcat tgtcgtcctc gggccagccc ttcggcgacg gcggcatcca
     1681 gatcggctgg ccgagggccg ctgaaagccg catgaactcg ccggctttcg ggcgatgacc
     1741 aagcccgcga gcgagggaga ccagaaaatc gtagggcggt acgatcttga cggcgggcgt
     1801 ggaccaggcg gccggatgcg cgacgagcgt tcgggcgagc gccgccaagt cgccgccggt
     1861 cttcagaaag gtctcggcga gctccgcgac cagcgcctga ggcggcgcct cggagacgaa
     1921 gtggcgggcg agctttagcg cgatgtggcg ggcggtcgcc ggttgtcgcg caatatcttc
     1981 gagcacggcc tcgcccgtca tgacaccctg gtcgaggtag gtcttgccga gaacggtcca
     2041 gttgccaggc tcgtggcggt tcgcgacgaa aacgaacttg cccgtctcgc cgatctgggt
     2101 ctgcggtccg ccgaaggtcc atccggtcag gatgcgcgca agattggtca cgtcgacctg
     2161 cgaatagccg ccgtcgacgc cgagtgtgtg cagctcaaga atctcgcgcg cgaggttctc
     2221 gttgaggccg cgcttgacgc gcaggccggc gatgctgttc gggccgatcg aaaactgatt
     2281 gtcgaggtag atcaacatgg cgggatgctg ctcgaccgcc ttcagcatgt cggcgaagcg
     2341 gccgaggacg tgcgggcgga tcgcctcgcg ttcgaaggcg ccggcgagcg cgcgcacatt
     2401 gcctttcgct gccgagacgc agaagtgggc cgaccagaac atgacgagcc gctcgatgaa
     2461 cggctggtcg gacgtgatgg cgcgttcgag ccgggcggcg atctcgtcct gcagtgcgag
     2521 attgcgcagg cgcccaaggc ggccgagcgg gtttggcgca agtttcggcg gcgccggcgc
     2581 gccagcagca ctcgagccgg ccggggctgc tggtccggcc ggtggcgcgc ccttcgcctt
     2641 gtcggagccg acaacgccct tggcctcgag ccttgcggtg cgaatggccg actgcgccgt
     2701 caggacatcg gtgaagatga cgtggctcgg atcgaggtcc ggatcgtcga ggagggcggc
     2761 ttgccgatcg gccagcgccg caagcacata gccgcgtgga tcggcagcga tccgggcgtg
     2821 atcgcccggt cttgcgccga gcccgaaacg gcggagcgcc gtcgccgtgt cctgcttggc
     2881 catgagacat ccctcgcatt ccctcggcag cccccgtttc gggcaccaat tgggccaatg
     2941 aacggcagaa aggttgccga tccgtcgcga tcacccggcg gcgacggcaa ggatcagcga
     3001 tgggacacgc agcgaagcga ccgcgatcag gccggctcgg ccctggcctg cagcattccc
     3061 gtgtcgagaa cggtgaggaa gcggcccgtg ttgatggcaa tgacgccgtc gtcggagagc
     3121 tggcggagct tgcgggcgac cgtctcgagc gtgataccga ggtagtcggc gatatcggtg
     3181 cgcgagaggg ggagctctat gcgctcgccg gcgctcgtgc cagtcgcacc gcacgccagc
     3241 ttccggtgct tcgcgaggca cagcatgatg aagctcgcga cccgctcctc ggctgtcttc
     3301 cggccgagca gcaccatcca gtcgcgagca tcctcgagat ccttcgcgag acgctgcatc
     3361 agctgggtct gcacggccgg atgtgcgcgc acgagcacat cgagctgcgc tcgcgtaaac
     3421 gtgcacaatt cgacatcggt cgcggcttcc gtcagcacgg cgttctgtgg tgacgtcggc
     3481 aaccccagga agtcggaagc gaactgcagc gcgacgatct gcgtccgccc gtcggagagc
     3541 gccttcgagt ggcgcacgac accatgcacg atactggcga ccagctggcg atcctcgtga
     3601 cccgaaaata tctgcgcgcc tgcgtgatag cgcctccggc gggcaatctg attgagttgc
     3661 gcgagtgcgc cgtccggcaa cgcccggcag agggcggcat cacgaatggc gcaagtgcgg
     3721 caggacgtgg gaagcatggg cctggctttc gaatgccctg gaggcgcacg gcgccaagtc
     3781 ctggatgggc gtggcagcgc gcgctggtcg cagcagcgag gcacaagttg ccgcagcagg
     3841 gtttgatctc gatcaagcgg cacggatcgg cctgctccag gttgcagcca cgattggggc
     3901 gcgtcgcgtg cgcgctcggc cgggagcagg ttgggggcga cgatatggcg gtggcagaac
     3961 acacacacgc gcatgatcac ccgtcgggct ggcggcgctg gctgctgtcg accaaccaca
     4021 aggacatcgg cacgatgtac ctgatcttcg ccatgttcgg cgggatcgtc ggtggcgcct
     4081 tgtcggtggg catccgcctc gagttgcagg agccgggcct ccaatacttc ggctcaaccc
     4141 acatgttcaa cgtgttcacc accggccacg gcctgatcat ggtgttcttc atgatcatgc
     4201 cgtcgctctt cggcggcttc gggaactggt tcgccccact gatgatcggc gcgccggaca
     4261 tggcgttccc gcgcatgaac aacatttcgt tctggttgct cccgtcggcg ctgacactgc
     4321 tcgtcatctc gctcttcgtg cccggcgccg ggccgtgcgt cgtaagcctc gatgcggcaa
     4381 cccgcgacct ctcgggctgc ggtgccggca tcgggtggac ggcctacgcg ccgctttcga
     4441 cgaagggcca cagtggaccg gccgtcgact tcgcgatctt gtcgctccac gtcgcgggcg
     4501 cctcgtcgat cctgtcgtcg atcaacttca tcacgacaat cttcaacatg cgcgcgcccg
     4561 gcatgacgct gcacaagatg ccgctattcg tctggtcgat cctgatcacg gccttcctgc
     4621 tgctcctgtc gctgccggtg ctcgcgggcg cgatcacgat gctgctgacg gaccgcaact
     4681 tcggcacgac gtttttcacg tcatcgggcg gtggtgatcc gctactcttc cagcatctct
     4741 tctggttctt cgggcacccg gaggtctaca tcatcattct gccggggttc gggcttgtca
     4801 gccacgttat cgcgaccttc tcgcggaagc cgattttcgg ctatctcggc atggcctacg
     4861 cgatggtcgc catcggcgtc gtcggcttcg tcgtgtgggc gcaccacatg tatacatccg
     4921 gcatcaacgt caacacacaa gcctacttcg tgttcgcgac catggtcatc gcggtaccga
     4981 ccggcatcaa gatcttttcc tggatcgcca ccatgtgggg cggctcgatc gtgttcacga
     5041 cgccgatgct gttcgcgctc ggatttgtgt tcctgttcac gctcggcggg gtcaccggcg
     5101 tcatgctcgc caatgcgggc gtcgaccgag cgctgcacga tacctactac gtggtcgcgc
     5161 atttccacta cacgatgtcg ctcggcgcgc tcttcacact gttcgcgggc tggtactact
     5221 ggttcccgaa gatgagcggc ttcatgtatt ccgagccgct cggaaagctg catttctggc
     5281 tgaccttcct cggcgtgaac ctcaccttct tcccgcagca tttcctcggg ctcgccggga
     5341 tgccacgtcg ctatgtcgac tatccggatg cgctcgcagg gttcaacaag atctcgtcac
     5401 tcggctccta catgtcggcg ctcggggcga tcgtgttctt catcggcatt gccgccgctt
     5461 tccgcgcccg tcggccggca gaggcgaacc cgtggggcaa gggcgcgacc acgctcgaat
     5521 ggacgctctc ctcgccgccg ccgttccact cgttcgagac actgccacgg atcaaggatg
     5581 atgaccattg acggatgagg caacggcatc ccgaagggcg gacgccattc tctgcacatt
     5641 cgatctgtgg ccccatcgct cgcttggcac gtcgggcatg gcttggctgt tcctcgccct
     5701 gtcgaccggg ctcggcatcg gcgtcctccg ggccccgacg cgcgtgatgt ggccgatgat
     5761 cgccggcagc cttctgacgc tcgtcatcat ggccgtagcg ctatggcgca atcaacgctc
     5821 cgcgcggtac ggagagcgca tcgagatcgg tcccggcatc ctccggctca cgcgcttcgt
     5881 tcacggaggg cgccacgatg gaccggaatt ctcgacccat tgggtccgca tcgagattgc
     5941 ccagcaccgc cacgtcgagc gccgcatcga gcttgtcgaa ggcggcaggc gcgccgcgat
     6001 cggcgacttc ctatcgcccg aggagcgtga cgctctggcc gcagcattga ccgatgccat
     6061 ccgcacggcg cgatcggctg cgccccgggt ggccggctca atgccggcgg accaccgcca
     6121 gcctcagtgc acataggccc tggacaagcc gccctgggcc ggggcacaga gcggcccatg
     6181 agccacgaca aacccatgct gctcggccag cacgtcgcat tgccggcaag gcggccgtgc
     6241 tcgagcgcgt gccgaatccg catcccgata cgcgctacct cgcccgcttc accgcgcccg
     6301 agttcacctc tctctgcccg gtgaccggac agcccgattt cgcgcacctc gtcatcgact
     6361 atgtgcccga ccagtggctc gtcgaatcga agtcgctgaa gctctatctc gctgccttcc
     6421 gcagccatgg cgcgttccac gaggactgca cggttgcgat cggcaagcgc ctcgtcgcgt
     6481 tgctcgcgcc ccaatggctg cgcatcggcg gctactggta tccgcgcggc ggcatgccga
     6541 tcgacgtctt ctggcagacg ggtgtgccgc ccgcgggcct ctggctcccc gatcaaggta
     6601 tgccggccta tcgcggccgc ggctgacaca gcccgaaaaa tagttcttgc gaaggcccgc
     6661 gccggcccac atgaagccct tgacccgaac gcccaccgcg gcgagcccga cgagcgtgcc
     6721 gacatgaccg accttacacg tctgaccctc accgaggccc gcgacggcct ccgcgccaag
     6781 aagttctcag cgaccgagct caccaaggca ttcctcggcg ccatcgagaa ggccaacggc
     6841 gcgctcaacg cctacgtatt gccgacaccc gagcatgcac tcgcccaggc gaaggtgagt
     6901 gaccagcgcc tcgccgacgg cagcgcgcgt ccactcgagg gcctgccgct cggccacaag
     6961 gatctctact gcaccaaggg catccgcacg acggcctgct cgaagatcct cggcgacttc
     7021 aagccgacct acgaatcgac cgtcggccag aacctctggg acgccggcgc gatcatgctc
     7081 ggcaagctca acaacgacga gttcgccatg ggctcgtcga acgagacgag ctgcttcggt
     7141 ggcgtcacca acccatggca gcgcgtgcgc aacggcgcga tcgacaacgc tcggctcgtg
     7201 cccggaggct cgtccggcgg ctcatcggcc gcggtctcgg ctgacctctg cctcgccgca
     7261 acggcgaccg ataccggcgg ctccatccgc cagccggcgg cattgaccgg cacggtcggc
     7321 atcaagccga cctatggccg ctgctcacgc tggggcatcg tcgccttcgc ctcctcgctc
     7381 gaccaggcgg gccccatcgc caagacggtc cgtgatgccg cggcgatgct acgccaaatg
     7441 gcgagccacg acccgaagga ttcgacctcg gtgaacgccc cggtgcaaga ctacgaggca
     7501 gccgtcggcc agagcgtgaa gggcctccgc gtcggcgtgc ccaaggaata tcgcgtcgac
     7561 ggcatgtcca aagaggccga ggatctgtgg gcgaagggca tcgcctggct gaaggacgcc
     7621 ggcgccagcg tgcacgacat ctcgctccgg cacacgaaat atgcgctgcc ggcctactac
     7681 atcgtcgccc ccgccgagtg ctcctcgaac ctcgcccgct acgacggcat gcgctacggg
     7741 ctgcgccgtc cgggaaagga cctgatcgac acctacgagg cgacgcgcgc ggaaggcttc
     7801 ggcgcggagg tccgccgccg cgtcatgatc ggcacctacg tgctctcggc cggctactac
     7861 gacgcctact acctgaaggc gcagaaggtg cgcacgctca tcaagcgcga cttcgacgag
     7921 gcgtgggcca aggtcgacgt ggtgctgaca ccgacgaccc cgaaccccgc cttcgcggcc
     7981 ggcgagaagt ccggcgaccc gatcgccatg tacctcgagg acatcttcac ggtgaccgtc
     8041 aacatggcgg gcctgcccgg catctcggta cccgcaggcc tctcggccga gaagacgccg
     8101 atgggcctcc agctgatcgg caagcccttc gacgaggcga cgctcttccg cgccggggcg
     8161 gtaatcgaga aggctgcggg acggttcacc gtgccgtcgc ggtggtgggt atagagttcg
     8221 cttcgcgaag cggggctccc gccccacacc ccgggcggag ggacaccctc cgaaccaccc
     8281 gactttgtaa caaaagtcgg gcggtcaggc ggctgtcccg ccagcggggt tgcggggctg
     8341 gccctgcctc gcgcagcgaa ccgaccgacc tcaaaacctg aaccgcttgc cgacggccag
     8401 cggtgccaac accttgtcgc cgaaagcgtt ggcgagcgcg gtcatggcgc gccagccggt
     8461 gcaatgcccg gcgccgagga cagtgaggcc gaagccccgg agcgcctcga ccgtctcagg
     8521 gatgatgcgc tcgttggtgc ctgagagatg cagcccgccg agggctgcgt agagcggtac
     8581 gtccggcatc atcttccgcg catgcgtgag cacgttgacg atgcccgcgt gcgagcaggc
     8641 cgagaggacg acgaggccct tccccgccac attgaaggcg agcgagcgct cgtccatcag
     8701 aagctcgtcc ggctcccagc ccgtgccatc gagcgtgcgc cgcacctgtc cggggaggcc
     8761 cgtctcgtag ggcgtcacgc gggcgatctc accgctgacg accgcgagcc cgtcgatcaa
     8821 tgcctgcggc tcgcgcgagg agacgacgga ggcgccattg gcctcgagtg ctgcaatgga
     8881 cggcacgtcc ttcatcggcc ggatggcgcc gtcggcgagg cgcgccgcgc gcgagcggaa
     8941 catctcggga tgcgcgtagt aggcgacggt cttgccgcca ttacggtcgc ggatcatctg
     9001 cagcgcgcgc agcatgccgc ccgcgtgatc ccagtgcccg tgcgagagca cgatcgcctc
     9061 gacaggggcg atatcgatgc cgaggcgcga gacgttctgc tcgaagacgc gatcttcggg
     9121 accggaatcg aagaggaccg tgcgcgtctc gtcaccgcgc ctcagcgtga caaggcagga
     9181 gaggccatgc gccgcgcagc ataggcaatc cccgtcgagc acccaggcgc cgcgccgccg
     9241 gcggctcagc gccgccgtct cggtctcgac gaaagcgggc gtcgaggaga ggttgtccgt
     9301 gacgttgtcg acgatgatct ggatgtcgag gccgtcgacg gcgataggcg tgggcatggc
     9361 ggagatccgg gtggcggtgg gcaggtggca aggtagcggt ctgccgcgcc cgcagccagg
     9421 ggggctgagc aggcggcgct tacgatcgtc ccgtcacgac gagggacgta cgcaatgggc
     9481 cagaagcaga tattgtgcgt cttattcata ttgcgctgca caccaacccg cccaagcaga
     9541 agcggacgtt tccgatgatc gcctggcgca caatatgtgc atctctgtca attgctgcta
     9601 tcggcggttg cgtccaggag aataccattc catttggatg cagcctttat accgccatcc
     9661 ctccctggaa gcctgctttg gtgcgcagtt cggatcgcgt atcttatgcg cgttcgttga
     9721 ttcgctgagc ttgatctatg gaaacgctga cgaagatcga tgttgctcgg cggcagctgg
     9781 tgacagccat ccgcctactg ttcgacgatc gcgatccggt ctcggtctat tccctggcca
     9841 caaacgccca agagatcctt tcgactctct gcgagcggcg cggggtactt tccttgcgcg
     9901 ggaatattgc cgctccaacg ggcatgaaca gcgatcgaat ccagacacaa ctaatcaatc
     9961 cggcgcggaa cttcttcaag catgccgatc gagatccaga ttccgtctgg cgcgatttca
    10021 ccgacactga ttgtgatcat atcttgctga ttccgagcat cgacttcgtt atgttggaac
    10081 ggaaatcgcc aatcgaggcg caagtgtttg tcacttggta cgcagctcta tacccggaca
    10141 aaattccgca aaacaccgat tggagtgacg cggcggcgag acatttttcg ggtatccgcg
    10201 ccgcacatcg cagtgaccag aagcgggccg catctgcgat tatcgctacc gcactcaaga
    10261 atcctgccct gatggcgcac cctgagactg ttccgagtga ggttcggcgg tggtcatagt
    10321 tcgcaccgca atcattacga cgggggcaac aggtggtcgg taatcgtact tctggccgtc
    10381 agcgaattcg atggtgcctg cccaatcggc gatcatctga tccagtcccc gcgcacgttg
    10441 cgccaaattg tcgagatcaa tgctatgtga accgacactt aactcgagtg agcggacgaa
    10501 ccgcatctct tgtctgtcga acacgaggcc cggcacgctc gctcgaaggt gcagagtacc
    10561 ggtgcagagc gagaactgcg tcaggcgatc ggctgcactg acccatgaaa tcgagcccca
    10621 caacggctgc gcttgaccag tgatcctggt gacttcgttg ttgatgtgct ggacatagtg
    10681 acggagcggc tcaacggcac tcgtgccgtc cttgaagacg cggattgcgg gctcgttctt
    10741 cgatagcgtc ggtgtgcgct cgatcaggag ccggatgcgc tgcgtggcgt cgatcatcat
    10801 ccatgtgtcg gccacaacct gcataacggc gctggaagat gctcctgact gccaatcggc
    10861 ctcgaaggag ttcagggcag atcgaactcg ggcatgacaa gaaagaatga tggcgaacga
    10921 atagcgcaac ccatcaaggc acagcagcag ctgctggacg cggttcggat cgacttcttt
    10981 gcggcgctcg gtcatgcaga atagctagca gattcgacac tatgcaacgc atccgcttcc
    11041 cgaggtattg ctaccccaac gcggcacgcg atcttgcgtg tgcaccagta atcgcactat
    11101 gtcttctaaa gtctgcggcg ccgccggccg tgcacgtcgc aatgctgcac ctgctcacga
    11161 ccgcaacggc tcaacgccga ccgtccaagg ccctctccgc actttcggtt ttcataccgg
    11221 ggagcttcgc cgggcacgcc cacttacccc cgctcccacc tcgcctcggc cgctccgtca
    11281 ctctcgcgcg cgtcgaccca gtcgccgctg ccgtcgagga aggtctcctt cttccagaag
    11341 ggggcgcggg tcttcaggta gtccatcagg aattcggcgg cttcgaaggc ggcttggcgg
    11401 tgcgcggagg ccgtgacgac gaggacgatg ttgtcgccgg gcttcagtgc gccgatgcgg
    11461 tgcacgatca gcgacgcctc gatcggccag cggcgccgcg cgtccgcctc gatgcgggcg
    11521 agctcgcttt cggtcatgcc tgggtagtgc tcgagcgtca tcgaggcgag cgggcggccc
    11581 ttgacggtgc cgcgcacgac gcccgtgaag gtgacgattg cgccgagatc ggtgcgaccc
    11641 gcggtcaagc gtgcgacctc ggcgccgacg tcgaaatcgt cctcaccaac ccggatcatg
    11701 gcatcactcg cccttggcgc gctcctcgcg ctttgctttg aggcgctgat cgacataggc
    11761 gccgacgacc tcggccagct cttccgtctt gctctcgaag aagtggttgg cgcccggcac
    11821 cacctcgtgg gtgatcttga tgcccttctg cgtcttcgtc ttggcggcga gttcggcgac
    11881 ggcggcggtc ggcaccacgc gatccttgtc gccgttgacc aggatgcccg acgaggggca
    11941 aggcgccaag aacgtgaagt cgtataggtt cgccggcggg ccgacgcaga cgaagccgtc
    12001 gacctcgggc cggcgcatga ggagctgcat ggcgatccag gtaccgaacg agacgcctgc
    12061 gatccaggtg ccgccagcat cctggttctg gagctgcagc caatcgagcg cggaggccgc
    12121 gtcggcaagc tcgcctgggc cgttgtcgaa gtagccttgg ctccgcccga caccgcggaa
    12181 attgaagcgc agcaccgaga agccacggtt aacgaaggcg tagtagaggc tgtagacgat
    12241 cgggttgttc atcgtcccgc cgaactcggg atgcgggtgg aggatcagcg ccaccggggc
    12301 ccgcgggcgg ccgtcgtggt gataacgggc ctcgatgcgg cccgccgggc cattgatgat
    12361 cagttcgggc atggtgcgct ccacctcgac ttcgcttgca tatgtaggcg cccgatggtt
    12421 ctgccaagcc ggcagaccgg gcacagttta tacttgacgg cgacgctcgg aatttccata
    12481 tatccggcaa gtttagaata gttcgaacct cagggtagcg gcaccggcat cgtcgatgcc
    12541 cgcacgtcac ccaaaggttc ggacgtaggc ctccgctcgg ggcgagggtc gcgagcagtt
    12601 ttcgacgggt ggtcggtccg gccacgcgcg gcggtcgcac ctcgttgccg aggctttggc
    12661 ggcggctatt agcacattgg cgcggcagcg gcgtatcgaa acgtcgcgcg ggcgaaggtg
    12721 tcgcgttgat cggccggggc ggcgcgtctg tgtggagctc ggtggcgtgg aactgaatac
    12781 caaaggccgg tacgcggtga cggcgctcgc cgatctcgcc aggcatgcgg gcgaggaggc
    12841 ggtgccgctg tcggtgatcg ccacgcgcca gggcctctcc ctcgcctatc tcgagcagct
    12901 cttcctgaag ctccgccgcg caggccttgt cgagagcgcc cgtggcagga ccggcggcta
    12961 taagctctgc cgccccgccg agagcatctc ggtcgccgag atcatgctcg ccgtggagga
    13021 gggcgtgcga atgacccgct gccgcggcga cgatagcgcg ccatgccagg cgggcgagcg
    13081 ctgcctcacg cacgatctgt gggatgcgct cggcgccgag atcgcccgct tcctcgatcg
    13141 cgtgacactg gccgaagtca tcgacggctc gctcggcgag cgacgccgcg catcgcgcgc
    13201 atcaggaaac gcggcctcga caggcccgcg gggagcgccg gcgaatggct gacgcgcgcg
    13261 cctatctcga ttggaatgcg tcggcaccgc tcaggcccga ggcgcggtcc gccatgctcg
    13321 cggcgctcga tttctgcggc aatccctcat cggtccatgc agagggtcgc gccgcgcgcg
    13381 caatcgtcga gacgtcgcgg gagcaggtcg cagcgcttgt gaatgcccgg ccgagcgagg
    13441 tcgtgttcac gagcggcgcc agcgaggcga acgcaacggt gctcgccgcg ccgtgccggc
    13501 atgtgatcgc ctcgcggctc gagcatcctt cgatcgccgt gccggcggcg cagtcggccg
    13561 attttgtcga gctgcgcgcg ggttcggagg gcatcgttgc ggccgaagcc atcgccgatc
    13621 tcgtgcttcg cacgacgcgg attgcgcccg agctgtcgct cgtcaccctg cagctcgcca
    13681 atggcgagac gggcgtcgtg cagcctgttg ccgagcttgg ggcgttctgc cacggccacg
    13741 gcattcatct ccactgcgat gcgacacagg ccgcgggacg catggtcgtc gactgcgagg
    13801 cgctctcggt ttcgacactt gcgctctcgg cgcacaagat cggcggaccc aagggcgtcg
    13861 gagcactggt catccgcgag ggctgcacgg tgccggcact catcacgggg ggtggccagg
    13921 agcgtcgccg ccgctccggt acggagaatg tcgcaggaat cgccggcttc ggtgccgccg
    13981 ccgctgccgc gcgcgccgat ctcgcggacg gcgcctcggc gcgcgttgaa attgtgcgag
    14041 accgcatcga gcgcgggctg agagccatca ctcctgacct cgtcgtgatc ggcgaaggcg
    14101 cgccgcgcct cgccaacacg tcgtgtgtcg ctttggccgg gtcacgctcg gaaacgctgg
    14161 tgatccgtct cgatctcgct ggcgtcgcga tcagcgccgg agcagcgtgc tcgtcgggaa
    14221 aggtcggccg gagcgccaca ctcgaggcca tggggctttc gcccgaggtc gccgggagcg
    14281 cgatcaggat cagcatcggc gcggccacga gtgatcggga gatcgacctc ctgatgcacg
    14341 cgtggtcggc cattcacgca acatcgggtg ttgaggcgag gccgcggcgc atgcacgccg
    14401 acctctcgcc ggaaccgcgc cccttcgcag tcggagagca ttaggccatg gccgcagttc
    14461 agcagacgat cgaccaggtg aaggccctcg acgtcgacaa gtacaagtac ggcttcgaga
    14521 ccaagatcga gagcgtgctc gccccgaagg gcctcaacgc tgatattgta cgctacatct
    14581 cggagaagaa gggcgagccc gagtggatgc tcgcctggcg gctcgaggcg tttcggcgct
    14641 ggcaggccat gaccgagccg acatgggcca aggtcagcta cccgaaaatc gacttccagg
    14701 acctgcacta ctatgcagca ccccgctcga ccgaagggcc gaagtcgctc gacgaggtcg
    14761 atccggagat tctgaagacc tacgcgaagc tcggcattcc gctcaaggag caggagcttc
    14821 tcgcgggcgt gagaaagccc ggcgaaccga gccacatcga cgatgccgag ggctttgcca
    14881 gcatggccgg caaggtcgcg gtcgacgcgg tgttcgacag cgtctcggtg gtgacgacct
    14941 tcaagaagga gctcgccaag gcgggcgtga tcttctgctc gatctcggag gcgctgcgcg
    15001 agcatcccga gctcgtccgc aagtacctcg gctccgtggt gccgaccacc gacaactact
    15061 atgccaccct caactcggcc gtgttctcgg acggctcgtt cgtctacgtg ccgaagggcg
    15121 tgcgctgccc gatggagctt tcgacatact tccgcatcaa cgagaagaag acgggccagt
    15181 tcgagcggac gctgatcatc gccgacaagg gctcctacgt ctcctacctc gaaggctgca
    15241 ccgcgccgat gcgcgacgag caccagctgc atgcggcggt tgtcgagctc gtcgccctcg
    15301 acgatgccga gatcaagtac tcgaccgtgc agaactggta ccccggcgac aaggacggca
    15361 agggtggcat cttcaatttc gtgacgaagc gcggcgactg ccgcggccgc aactccaaga
    15421 tcagctggac gcaggtcgag accggctccg cgatcacctg gnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnntctactc gatcgccgta tcgaatggcc gtcagcaagt cgacagcggc
    15541 acgaagatga tccatctcgg gcggaatacg tcgagccgca tcatctcgaa aggcatcgct
    15601 gccggccact cgcagaacac ctaccgtggc ctcgtctcgg cccatcggcg cgcaacgggc
    15661 gcccgcaact tcaccaattg tgacagcctc ttgatcggcg gaaagtgcgg ggcgcacacg
    15721 gtgccctaca ttgaggccaa gaacgcctcg gcccacttcg agcacgaggc gaccacctcg
    15781 aagatctcgg acgacacgct cttctattgc atgcagcgtg gcctcggtga ggaggaggcc
    15841 gtcgcgctcg tggtgaacgg cttcgtgcgc gatgtcctcc agcagctgcc gatggagttc
    15901 atggcggaga cgcaaaagct gatcgggatc agcctcgaag gcagtgtcgg ctgatgcagt
    15961 taagcaacaa tcccggtcag gtcgagcttt ggctcgatca tccggctaga ctgcggcgag
    16021 gtgcgacaat gtgtgcaaat tggactgggc aacggccaat ccagtcgcta ggatcgcccg
    16081 actttagtcg aagtgtgcgc ccgggaacgg tcagagcata agtgccgtcg gggcgagacg
    16141 agtgttgagt acgtggttgg aagcgcagtt gttctctacc gaggtgcaca cggcaccgcg
    16201 gatcaatcga aggagttggg cgtcatgacg gatgcggcgg gtatgggtga gctcattgcg
    16261 acggcgaagc tcgtcaggca gatcggcgag gatgagtgcc ggcgccggaa tgcgctctca
    16321 cggaccgttc ccggctttac gctgaaaccc ggccagatcg gcaacgttct cgagaaattc
    16381 ggcgagggcg aggcgtttct cgtcgagttc ggaccgaagg ccaacagttg cgactggctc
    16441 ggtgtgctct actcgagcga gcttgagctc gtgtcttcgg cggatatccc ggccgcgcgc
    16501 atcgtcaggg ccgcctgatc gccggccgag cgacctcacg aagggagcgt ccgacaggtc
    16561 cgcagccgca ccggtttcag cgtgagggca cgttgaaact ggagcggatg ctgatccgga
    16621 gggctgttgc gtggagcatt gcctggcttt cgatggcagc ctgtcaatcg aggcggcaga
    16681 ggcatcggcc ggatctcggc agatcctgcc ggggttgcgg cggcgcctgc tggccggcat
    16741 gtcagcgttc agctgcggag ttgggccgag gcggtgccgc gacgatcttg tatgccgact
    16801 tgatgcgccg acttggaatg agccggacga cgcgagcggt gagccaagtc ggctgcctgc
    16861 tcgtattact gggcgaaagc cgcaacggcg agagcacacg gtccaatgcc gcaagctagg
    16921 cggcgaggac tgctgctcat gctcgagcgt attgcgctcg aaggcgcgag cctgccgagt
    16981 ggccacgcac gacgtcagcg cgcgaagtgc gcgaacagct ggacggatca ccgagcgcgc
    17041 gccgtcgcgc ccggcatgga atgaggagca tgtgccgccg tgcggcccgt gcgagaaaga
    17101 ttcgggagca ggtgaatatg cttgagatca aagacctcca cgtccgtatt gccgaggagg
    17161 acgtcgaaat ccttaaaggc gtcacactca ccgtcccgaa gggcgaggtg cacgcgatca
    17221 tggggccgaa cggctcgggg aagtcgacgc tcgcctatgt gctggccggc cgcgaagact
    17281 acgagatcgt cagcggcgac atcctctggc aaggagagtc tctcatcggc atggcgccgg
    17341 acgagcgagc cgcaaggggc gtcttcctcg ccttccaata ccccttcgag ctgccaggcg
    17401 tcgcgacgat gacgttcctg cgtaccgccg tgaacgcagt ccgcaagaag cgcggcacga
    17461 aggaactgac gacacccgag ttcatgaagc tcgtgaaggc tcgggcagcg gaactcggca
    17521 tctccgacga catgctgaag cgcccgttga acgtcggctt ttccggcggc gagaagaagc
    17581 ggatggaagt gctgcagatg ctgatgctcg aaccgtcgct cgcggttctc gacgaaaccg
    17641 attccggcct cgacatcgat gctatccgga tcgtctcgga aggcgccaac aagctccgct
    17701 ccaaggaccg cgcgcagctg gtgatcacac actaccagcg cctgctgaac tacatcgtcc
    17761 ccgatcgcgt ccacgtgctc gccgagggcc gcatccagaa gtccggcggc aaggagctgg
    17821 cactcgagct cgagaagtcg ggctacgccg gcttcaggag caaggcggca taagcgcggc
    17881 gacgcccatg gtccaatgag gcccgctgct cgcgcaggga tgcccatccg ctggtgcgcc
    17941 cgcagttggc acgggtgcga agcagggctc ggggccggcc tgtcggatca tcattgcgag
    18001 aaataccgag acgaccatca tgacgacgac cgagaagaca atggccgaga tggcgctcgc
    18061 cgccgaattt gaccgcatca tcgaccggct gccgggcgag gggctgcgcg atgagcgccg
    18121 cgcggccatg gcgcgccttg cggcgtcagg tcttccgcat cgccggatcg aggaatggaa
    18181 gtacaccgat ttacgcgccg ggttgaagga ggtgttcgcc gcggccgatg gcgccaacgg
    18241 cgtgaccgca gctgacgtcg ccgcagccct cggcccgctc gggacgctcg aggcgcaccg
    18301 cctggtgttc gtggacggca tctatgcccc cgcactctcg ccgctcgatg ctctcggcac
    18361 ggacgcaatt gcggtcaagg tcgcggcagc aagccagacg ttcgtgccga ccgccaaacc
    18421 gatctccgaa ggcgcccgcg gcgatgctct gcaggcgctc aatgaggcgt ttgcgagcga
    18481 cggcgccatc atttcggttc aggacgacgc aagcacatct gcgcgcgatg ccaaagtgcg
    18541 cagtggatcg ccggcgaagc cgatcatgat cgtgtcgctc agcacggcgc gggcaccgct
    18601 tcgcgtcacc acacggcatc gcatcgagat cgatgccgga atgacggcga ccgtgatcga
    18661 ggtctacggc cgcatcgggt cttctgcgat ccagaccagt gccggctcga tcgtccggat
    18721 cggcaagggc gcgaagctgc atcatcacaa gttcgcactc gaaggctcca ggggcgccca
    18781 tctctcgagc tcggatgtcg agatcggagc gggggcgacc taccgcgctt tccatctgac
    18841 acccggatcg ggcctcgcgc gcaccaatgt gtttgcaacc ttcgcggggc cggacgccag
    18901 gctcgacatc tcgggcgctt tcctcggcgc cggcgcggat catgtcgata cgacgctcgt
    18961 catcgaccac gccgtgcccg gctgcgaaag ccgcgaactc ttcaagggca tcctcgcagg
    19021 cgatgcgaag ggtgtcttcc agggcaaggt gatcgtgcgg cccgacgcgc agaagaccga
    19081 cggcaagcaa atggcgcaag ctctcatgct ttcgcccacg gccgagttcg acagcaagcc
    19141 cgagctcgag atctacgccg acgacgtgat ttgcgggcac ggttccacga ctgccgaact
    19201 cgatgcggaa ctgatgttct actgccgctc ccgcggcatc cccgaggccg aggcgcgcac
    19261 gctgctcatc gacagcttca tcggcgaggc gcttgctaag gtcgaagatg gcgagctgcg
    19321 cgaggcctgt ttggagatcg tcgccggctg gctgaagacg aacctctgag cctcattctc
    19381 cccagctccg gccatcccga gcattccgag accacggtag agaccatgag cacgccagca
    19441 acacctgatc gcgcgatccg caccggcggc agcggcgcct tcgatgtcga gcgcatccgc
    19501 gccgacttcc cgatcctctt ccgcgaggtc tacgggaagc ccctcgtcta cctcgacaac
    19561 gcggcaagcg cgcagaagcc gcgctccgtg ctcgacgcca tggaccacgc ctacaagttc
    19621 gaatacgcga acgttcaccg cggcctgcac tacctctcga acaccgcgac gtcgcgcttc
    19681 gaggaagcac gcgccatcac acgccgcttc ctcaacgcgc gctacgagga ggagatcgtc
    19741 ttcaccggca acgcgacgga cgcgatcaac ctcgtcgcct cgagcgtcgg tctcagcctc
    19801 gcggagggcg acgagatcat cgtctccatc atggagcacc actcgaatat cgtgccgtgg
    19861 catttcttgc gcgagcgcaa gggctgcgtc ctcaagtggg cgcaggtctc ggacagcggc
    19921 gagttcctgc tcgacgagtt tgcccggctg atcactccga agaccaagat cgtggcgctc
    19981 acccaaatgt ccaacgtgct cggcacggtg acccctgtga aggagatcgt gcggcttgcc
    20041 catgagaaag gcgttcgcgt cctcatcgac ggctcgcagg cggccgtgca catgcccgtc
    20101 gacgtgcagg acctcgactg cgacttctac gtcttcacgg gccacaaggt gtatggaccg
    20161 tcgggcatcg gcgtgctcta cggcaagaag gcgatcctgg acagcctgcc gccctatcgt
    20221 ggcggcggcg agatgatcga ggccgtgcac gtcgacaaga tcacctatgg caagagcccg
    20281 caccgctttg aggcaggaac accagcgatc gttccagcga tcggcctcgg cgccgcgctc
    20341 aattacatga tgcaggtcgg ccgggatcgc atcgcggcac atgaggcgga cctcggcgcg
    20401 tatgcgcatg cccgcctcgg cgagctcaac tggctgaaaa tccatggccg ggccaagggc
    20461 aagggcgcca tcgtcgcctt ctcgatcgac ggcatgcacc cgcacgacat ctcgacgatc
    20521 atcgatcgct cgggtgtcgc ggtccgggcc ggtcaccatt gcgcgcagcc gctgatggag
    20581 cgtctcggcg tcacggcgac ctgccgcgcc tcgttcgcca tgtataatac gcgcgccgag
    20641 gtcgatgcgc tcgccaatgc cctgatcaag gcacacgatt tcttcgcttg agacggcggc
    20701 cggtgtatga tgctgccgtc cgaccaggac acgtcatgaa ggaagggcgc tcagcccatg
    20761 gatggcaagc cgatggagag caagacgatc gagcgcgcgt cgaccgagcg cgacgtgctg
    20821 agcgtcgacg cgggtgacgc ggcggtggac ggcgcgccgc gtctgacgcc gccgacggtt
    20881 gctgagggcg gcacgccggt cgcaggctcg acgctctccc aggaggagct cgagcagatg
    20941 acggcggaca tcgttgccgc cctcaagacc gtctacgacc ccgagatccc ctcggacatc
    21001 tacgagctcg ggctcatcta caagatcgat atcggcgata accgtgacgt tgccgtcgaa
    21061 atgacgctga cggcgcccgg ctgcccggtc gcgggcgaga tgccgaagtg ggtggagaac
    21121 gcggtcaaca ccgtgccggg cgtcaacaac accacagtga cgctgacctt cgacccgccc
    21181 tgggaccaga gccgcatgtc ggacgaggcg cggcttgccc tcaacatgtt ctgagccggg
    21241 ccgccgcgca gctccgctgc gcgatgcagt catttgcgag tgagacatgc ccgagacctc
    21301 tcgcgccgag gcccgcatcg tcgaggaggt ggcgcttgcc cgctcgatcg tgacctatca
    21361 cggggatgca gcgaagaccg cggccatgga ccacctctac agccggttcg tgaagccggg
    21421 cgacctcgcc ttcgacatcg gcgcccatgt cggcgaccgg acggcatcgt tcctccgact
    21481 cggcgcgcgc gtcgtcgccc tcgagccgca aggggggcct gcagccgtgc tcgcctgttc
    21541 ggcgacgacc agcgcgtgac gctcatcaac gctgcttgcg ctgccacggt cggcagggtg
    21601 accttccgca tcaactctgc caatccgacc gtgtcgacgg cctcgcgcga gttcgtcagc
    21661 gcggcggacg gggccggcgg ctgggaaggc caggtctggg atcgtgaggt cgaagttccg
    21721 gcaacgaccc tcgacgaact catcgcccgc catggcacgc ccgcgttcac gaagatcgat
    21781 gtcgaaggct tcgaggcctc cgtgctcgcc ggtctgacgc gggcgctgcc ggcgctatct
    21841 ttcgagttca cgaccatcca acgcgacgtg gcgctcgcct gcctcaatcg tctcgccgca
    21901 ctcggcgcct atcgcttcga catnnnnnnn nnngccctcg gcgagagcca gtccatgacg
    21961 ttcggccggc cggtctcgcg cgacgccatg gcggcccaca tccgcgatct tccgcacgcg
    22021 gcgaactcgg gcgatgtcta cgcgatcctg gagcggtgag cggcggctcg ccttgaccct
    22081 tgcggccggg ctcccctacg gtcacgccca aaccagtcag ccacagcacg agttcggagg
    22141 agcgcccatg gcccgcgagt acctgaagaa ggctgccgtc agcaaggcga tgcgaaaagc
    22201 cgaggtggag gagaccgtgc ggcgcatgct cgccgacatc gaggtcaacc gcgatgatgc
    22261 ggtcaggaaa tacgcgcgcg aactcgacaa gtggacggcg gactttcgcg tcacgaaggc
    22321 agaggtcgag gcaaagtcga agtcgctctc aaaggtattc aaggaagact tcgcgctctg
    22381 ccacaaaaac gtgacggagt tcgccaaacg ccagaaggac agcatgcgac ctttcgagag
    22441 cgagctcacg cccggcatca tgctcggcca gaagctgatc ccggtctcct ctgtcggctg
    22501 ctacattccg ggcggcaaat atccgctcat ttccgcggca atcatgagcg tcggtacggc
    22561 aaaggtcgcg ggcgtcgggc gcatcgtcgc gtgtgcgccg ccacgcggtc cggatggcat
    22621 ctatccccag acgctctatg cgttgcacta ctcgggagcc gacgagatct actgcctcgg
    22681 cggcgtgcag gcgatggcgt caatggccca cggctgcgtc ggcatgccga aggcggacat
    22741 gatcaccggt cccggcaatg ccttcgtcgc ggaggccaag cggcagctct tcggcgtctg
    22801 cggcatcgat ctcctcgccg ggccgacaga agtcgccgtg atcgccgacg agggggccga
    22861 tcccgcgctc gttgcgaccg atctcctcgg tcaggccgag catgggcccg acagcccggc
    22921 atggctcatc acgacctcgc gggcgctcgg cgaggccgta gcgaatgaga tcgccaagca
    22981 gctgccgtca ctgccgacac gcgccaacgc ggaagccgct tggcgcgacc atggcgaagt
    23041 cgtcgtcgta gactccgatg acgaggcgat cgccgtttct gacgaatacg cacccgagca
    23101 cctcgagctg cagacgcggc gcgacgacta ctatctcgaa cgcctcaaga actacggctc
    23161 gctgttcgtc ggcgaggaat cgaccgtcgc ctacggcgac aagggcgtcg gcaccaacca
    23221 cacgctaccg accgagggcg ccggccgcta cacaggcggc ctgtgggttg gcaagttcat
    23281 caagaccgtc acctaccagc gcctgacgcg ccgtgcgtct cgcgagatcg cccccgccat
    23341 gagccgcatc tgccgcgagg agggcatgat cgcccacgcg atcacgggcg acgttcgcgt
    23401 caagcggttc caaggcgcga actgacgatc gcgggcgcca aggcggcccg ccaccggagt
    23461 cgccaggccg aagcttgcgc ttggcgaccc caccccggtc tcttgcacca acagcttgtc
    23521 gtatgccctc atcgatgggc tcctccacca caaccagcgt cagataagcg cccttcagat
    23581 cgcgcaaatg gaaatatcag tttctccaaa tatggaattg cgggttcgac tgtggtggct
    23641 caagaccggc gagatgtccg gttcttcggt gccgtttgcc tacaagggat ctgggaagtc
    23701 caaattgcag ttcatcgata tctccatgat atcgcatggt aatttgattt tcatcacccc
    23761 cagatggcaa agtgcgaata cacaagtgct ccagatccac tcctcaagtc tgatttaagt
    23821 cagtgccact cggaagatcc catttgcaat ctatggtggc tgcttcggcg gaaaagacaa
    23881 tttacggatg catcgttctg cttttgttga gcaatccaga tttgctcgga aggcgaaaat
    23941 ggcataatct gcccgaggtc aagcatcctg ggcatccacg tggcgacaag agcagaatgg
    24001 caaaatgatt tggacccatt gggaccgctg gggggcgggg gtgatactca gctcaaccgc
    24061 ttgtgcagtc tcatcgcacg gctgtgccgg gcgccctcgt gcgcgatcgt cggttcgagg
    24121 ggatcgcgcg actggcttat tgcgagcttt ggcctccatc atgcactcgg tcggtcgatg
    24181 ccgagcctca tcaacaagtt cgatttgtcg tccagtgtgc gctatataaa gaatgtaggc
    24241 caacacaagg cgctcatgga gcatcctctg ctgctggctt ctccgctcgc acgctcagta
    24301 ctcgtcatcg agattccagc cgtgccattt ggcgcacatg cggcgatgat catcatcgat
    24361 ccgtcgtcca ccatgaactc ccagctgatc aacgacctgc acgagggggc cgcccttgcg
    24421 agcgagttgt tccaccgagc cgacaatgcg gtcccaagca gcctgcgtcc gccgggcctt
    24481 gccgagggcg tccccgaggg tgccgtcacg gagttcctgc tggagaccct aaagactacg
    24541 cgctctctca agagcaggag aaaaacatcc tatctaacgt tgagaacgtg gcaaacaagc
    24601 attaagaaat tccagatttc cgcaataaaa tcattgaaaa aacaaccctc tccggaattc
    24661 gtatccgcga tagcctccga gttcatcaat gcgtctcaaa tgctcgtcgg caccaagggg
    24721 atcgacaccg tcgtgccgat gccttgtggc cactcgcgat cggacgagtg cctgtcggtg
    24781 cgcattgctc ggcgtatcgc cgaagcgtac cccctgacat ttcacatggc gttcaagtca
    24841 gtgcatcgca gcggctcgtc ccaccctcgc cggaacgtcg atctgccggc cttcaagctc
    24901 gtgtcgccac cgagcggcaa tgtcctgctg gtcgacgaca ttgcgaccac ggggacgcat
    24961 atcgaaaagg ccaaggagat cctcttgaag caggcgcgca acgttttggc cattgcctgg
    25021 atcggctgag cggcacacac agtcctatcg gcttatggcg ctcgacgcgc catctcgttc
    25081 accggacagg gatgtctcga cggtcggcga cgcggccctc gaccgtcagg cgatagatga
    25141 tcggcgcgcc ggtcgcgagt tcgcgcttga tgatcgcctc gcccgaaagg ccttcgaggt
    25201 gcatgatgag ggcgcgcagc gagttgccat gcgcggcgac gatgacggat tttccgtcct
    25261 tcacgtcggg ccagatccgc gccatgtagt acgggagcac gcgctcggcc gtgtccttga
    25321 ggctttcccc acccggcggc ggaatgtcga aggagcgccg ccagatgtgc acctgctcct
    25381 cgccccagcg cttccgcgcg tcgtccttgt tgaggccgga gagatcgccg tagtcgcgct
    25441 cgttcaatgc ctggtcgcga tgggtgggca ggtcgctctg gccaagctcg cggaggatga
    25501 gcgcaagcgt atgctgcgcg cggagcagcg cgctcgtata cgcgacgtcg aagcgcagcc
    25561 cgagatcctt gagcttgcgt ccggccgcgg tcgcctccgc cacgcccttc tcagtaagat
    25621 cggggtcttt ccagccggtg aacagattga gcttgttcca ggcgctctcg ccatggcgga
    25681 caagcacgag tgagttgttc atcttaccct ccggcagagc tgcatcctgc ggcaactgca
    25741 ggcgcgcgct gcagaaatca aggcgcgaaa ggccgcgggc gcatggctgg gtcgcggcaa
    25801 ttgctgcgcc agcaccgggc gaccaacact cacaatgcgt gctagcctgc cgctccaacg
    25861 aaccggagcc tgggggccag aatgacgcgc gagcgcaaat ccttcctgtt cctcaacgtc
    25921 ggccatttcc ttgaccacct cttcacgctg atcttcgcct cggtcgcggc gctggcactc
    25981 gtgaagcaat ggggcatctc gtacgccgag ctgaccctct acggcctgcc tggcttcgtc
    26041 gcgttcggag ccttctcgct ccccgccggt tggctcgctg accgctggag ccggcatggc
    26101 atgatcgtcg tcttcttcat cggcatcggg gcggcctcga tcctcgcctc gttcgcgcgg
    26161 agcccgctcg agctctcgct cgccgtgttc gtcgtcggca tgttcgccgc gatctatcac
    26221 cccgtcggcc tcgccatggt gatcgagcag gcgaaggcaa acggcactgg catgatgctc
    26281 ggtatcaacg gcatctgggg caatctcggc gtcggcagcg cggccctcat cacgggtgcc
    26341 gtcatcgatt ggcttggctg gcgttgggcg ttcgtcattc cgggcgtgct gtcgatcctg
    26401 gtcgggctcg gctactggca acatgcccgc gatgagatca aggccgccaa cgctcgcgtg
    26461 agccccaagg tgacgatcgc ctcggccaag gcggcggcca gcatgccgag cgatcatcgc
    26521 cgcgcgctca tcaggctcac ggccatcatc ttcttcacca cagccatctc gtcgatcatc
    26581 ttccagtcga cgacctacgc gctccccaag gtcttcgagg agcgtctgcc ggagctagca
    26641 ggcagcgcca ccaaggtcgg ctggttcgcc ttcgtcgtgt tcgccatcgc ctcggttgcc
    26701 cagctgatgg tcggcaaggc gctcgacgta tacggcccgc gcctcgtgtt catggtcgtc
    26761 gccgccgtgc aggtgacgtt tttctcgctg atgccgggct tgcgtgacct tcccgcattg
    26821 ctcgtcgcgc tcggcttcat gctcggcgcc ttcgggcaga tcccaatcaa tgactacatg
    26881 atcggccgca tggcgcgctc ggagctgcgc gcgaccatct atggcgtgcg ctacttcgtg
    26941 agcttcatcg cgctcgcagc gaccttgctc ctcgttgcct gggtgcacaa gaactggggc
    27001 tttgacgtcc tcttccgcat cctcgccggc acggccgcgc tgatcttcct cgccgtctca
    27061 ttgctaccga cgaggctgcc ggacgcggac gcggcaccgt tgccggccac ggccaaggcg
    27121 tgatcgcaaa aggccgtctc gccgcggccg ccgaatgcgt taccactcgc ccgtgaagcc
    27181 cgcgacattg gccgcggtga tcttcggcat ggggatcagg ctccgcttct ccttcggcat
    27241 ctgcccggca gcgagtgcga gcgcgatgtc gagcgcctgc ctggcatcgg cagcgggcga
    27301 ctgatcgacg gtgccgtaca tgaggcccga cctgatcgcg gcgaggccgt tcttggtgcc
    27361 gcccgtgccg acgaccttca catcactgcg gccgaccgct ttgaacacct cggccgccgc
    27421 cgcgccagag ttgtcgtcct gggcaaagag cccgacgagc ttcgcgccgt gtgccgtcag
    27481 gaatgcctgc gccacgtcct tcgccttcac cgggttccaa tcggcggtct gcgcgccgat
    27541 gacggcaatt tcggacttga gctcggccaa gcgatccttg agaccttttg tgcgcaggag
    27601 cgtcgtactc tgtccgggcg aaccctcgat gatgccgatg ccacccttgc cagcgagagc
    27661 ctcgtgcaac agctccgccg cgagccggcc ctgcacgtag tcgtccggcc cagagtaacc
    27721 cgcaagcatc gcctggcccg cctcggacgg ctcgttggag acggcaagaa tgggaatgcc
    27781 gacatctttg gcgcgcttca aggccggcac caccgcgttg ccgtcgagcg acgtgacaag
    27841 cagtaccttg accttgcgat tgacgagttg ggcgatgtcg ctcgactgct tggcgatgtc
    27901 gccgttcgca ctgagcgtga ccagtgtcac gcgcttcgcc ttggcctccg cctcgagaag
    27961 gcgcgtcaca gcagcgatgt acggccagcc gagattgggc tgcgagtggc cgatcacgat
    28021 cggggcctgt gcgcgagcag ggccaatcag acccgaggca agcgtcgcga gtgccgcacc
    28081 attgacggcg cgtctggtgg tgatcatgca gtctctcccg tggcccatgg cctatttcgc
    28141 agttgaggcg cgagcaagca tcccgtcggt gaccaccagc gcgatcagca tgcccccgat
    28201 cgccatgtgc tggtagtagg ccggaatgcc gagatgattg aggacattca cgagcgtcgc
    28261 gatgaacagg agacccgcgg cggcgccaag gatcgaacct tcgccgccct tcagcgtcgc
    28321 gccaccaatg agggcagcgg ccgccgccgt gagcggtgtg gcatcgccga tgatcggcga
    28381 gccggtgccg aggcgcgccg cgagaaacag tcccgcaagc ccggcgnnnn nnnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccccacggcc ggctcgacaa gctcagatgc
    28561 aacagcgtga gcacgagcac catcagtagg aatgcgtagg ggatcggatc accgccgccg
    28621 agcgccagga agcgctcatc gaggccgaca acaggtcgcg tccccgtgta ggcgaggagc
    28681 gcgccgcgca gcaccaccat cacggccaga gttgcgataa acggattgat gtgcagccgc
    28741 gtcacgaacg ttcccgaggt gacgccggcg agcgcgccga ggccgatcgc ccctgcgaac
    28801 gagaccagca atccataagg gaggagcacg accgacgcga cgccgccgag cgccatgacg
    28861 gcgccgacag acaagtcaaa gcccgccgct atcattacga tcgtcatgcc ggcgacgata
    28921 aatccgtcaa cagcgacgga ttcgagcagc acacggatgt tgccgatcgt ccagaacccg
    28981 tcgatgaacc aggcaagcgc cagcagcggt acaagcggcg gcttgatgaa gagcatgcgc
    29041 agccatggcc gcacgagcag gctcatgtcg caccctcccc gcggctccgc gcgtagaagg
    29101 cgacggcggc gacgagcacg atgccctgca ccatgagctg gctcgcgtgc tggtagttca
    29161 ggatggtcat cacgttggcg atgatcgaga cgatcagtat cgctgcggcc gtatcggcgg
    29221 ggcgcccgac gccgcccgca aggctcgtcc cgccgagcac cgcggcagtc agcgcgggca
    29281 gctcgtaacg cgcgatcgcc gtcggatcga tgacgccgac catcgccgcg atgatgacgc
    29341 cagcgagcgc ggcgagcgtg cccgagatca cgaatgtcgt gatgccgacg ccaacgaccg
    29401 nnnnnnnnnn nnnnnnnnnn nnncaatcac cgccgaccgc gtagatgtgg cggccgagga
    29461 cggtgctcga catcatcacg gcgaaccccg cgaccagcac cgcgaacagc atcaccggca
    29521 gcgggatgcc gagcggtcgc cagagcgcga gcccggtgaa gaccgggtga atactctcgg
    29581 cacgcacatg ctggccgccg acgagggcca gaaccgcacc gagcagcagg aattgcatgc
    29641 cgaccgtggc gacgatcgag ttcgcgccta gccgcgagac cgcaatcccg ttgaggaggc
    29701 cgaccgtggc gccgatcagc agcggcacca agaccgcgag catcacgccg gcgggcgtcg
    29761 acggccccat gagcagcgcc actacggcgg ccatcatcac cgtcgagccg gtcgagagat
    29821 cgaactcgcc gccgatcagc acgatcgtca tgccgagggc gacgagcccc gtgatgacac
    29881 cctgctgcag cacggcgacg atgttggcgg acgtcaggaa gcggtcgctg acgagcgcgg
    29941 cggcggcgat cacggcgacc aagacgagcc agatcggggc gacgccggcg agccgcgtcg
    30001 cagtcgctcg cagtagcggc gctgtttcgg aggcagaact catcgcgcgg cctcgggtcg
    30061 gatgcctgag ccctcgcgcc gcccgcggat gatgaggtcg accacctcgt cctcgctcgt
    30121 tgccttgacg tccagagtgc cgacgatctc gccgcttttc atgacgacga tgcgatcggc
    30181 gaggcgcatc acgtcgcgga tgtcgtggct gatgagcaga atgccgatgc ccttcgtacg
    30241 aagccgggca atcagcgcgc cgacctcggc cgtctcatga ggtccgagcg ccgccgtcgg
    30301 ctcgtccatc acgagaagct tcacgtcgaa atggatggcc cgggcgatgg cgatggcctg
    30361 ccgctgaccg cccgacatct ggctcacggg ccgctcgatg tcggtcatct gcggattgag
    30421 cgagaggagc gcctggcgtg cctctcccgc catgcggcgc ttgtcgagaa agccgagccg
    30481 cgtcgtaagc tcgcgtccga ggaagaagtt cgccggcccg tcgaggttgt cggcgagcgc
    30541 cagtgtctgg aagatggtct cgatgccatg acggcgtgcg tcgttcggcg aggcgagcgc
    30601 gatctcccgg ccggcaacct cgagccgccc gctgcttggc gcgaaaacgc ccgaaacgat
    30661 ctggaccagc acggacttgc cggcgccatt gtggccgacg agcgccacga cctcgcctgc
    30721 cgccacggcg agatcgacgc ccctgagagc cctcacgccg ccgaagctct tcgtgatccc
    30781 ggtcaacctg acgagtggat cgggccccat accgctgcaa tgccccctgt ccgacacccc
    30841 ctcgccatgg caacaggcgc gcccgcgcaa gtcggtagcg ctcggaccgc ccgatgcctc
    30901 cggcggcgca ccttatcagg acgcccttcc acagcaagat tgaccgacta tattggtcat
    30961 catgatgggt cgcggcaggc catcgcccgt caggtcgatc ttgcagccac tgcggcgagc
    31021 tgacgcgagg ccggccattg ccctgccgga aagatcaggt cctattcaag cccgtcgtcc
    31081 aaagccgggt cgccagtcga gtagagttcg tcgttcggga tgaagcgcgt cggtcgcgcg
    31141 tgcacttcca cccggggcca agcatggaat tgcagagagt tgcgtgcgca tctcacgccc
    31201 gcgcgtctcg tcgaaggcaa caacacatca tggcgggagt tcacccgtgc gagccgctac
    31261 cacaacagtc agaccgcgcc tcaatctgat ggcgcgcgct gctcgccgcg ccgtcagcgc
    31321 agtggctgct gcggccgtcg ccttggcgcc ggtcatggcc gaagcgcaga tgattccgga
    31381 ggtgcgcgac acggagatcg agcggctatt gcgcgagtac gctcagccga tcctgcgcgc
    31441 cgccgggcag gagaagggcc gcatccgtgt gcgcatcgta cgcagcgagg tcttcaatgc
    31501 cttcgtgatc gatggacgga gcatctacgt ccatacgggg ctcctcgttc aagccaagag
    31561 cccgaaccag gtgatcggcg tcctcgcgca cgaggtcggt cacattgcga acggacacct
    31621 cgcggcattg cgcgaccgca tccagcgcga ccagacgaag agcctgctca tcaagctgct
    31681 cggcatcggc ctcctcatcg cgggcggcac gagcggatcg gccggcacat cgagctcggg
    31741 tagtgccgtc ctctccggcg gcgacgagat cctgctccgc tcgctcctcg ccgagcggcg
    31801 ctcgcaggaa agctcggccg accagtcggg cctcaagtac cttttcgcca cccgccagtc
    31861 gggccgcggc atgctcgaga ccttcgaatt cttcgcgcaa caggagtacg tgtccgacca
    31921 gtacaaggac ccgttcgtgc gcagccatcc ggtcgcagcc gagcggctcg cgcggctccg
    31981 agacgcagtc atggcgagcc ccttctatgg cgtgaccgac ccgccggctc ttcagctccg
    32041 ccacgatctc atgcgggcga agatctcggg ctatctcgag cgcatgcaga tcgtattcaa
    32101 caagtacccg cggacggaca cttcgctgcc ggcgcgctac gctcgggcga ttgcgacgtt
    32161 tctcggcagc ggcctcgatc cggcgctccc tctcgtcgac gagctcattc gcgagaagcc
    32221 ggacaacccc tacttcctgg aggtgaaggg cgacttctac gcgcggagcg ggaaagcggc
    32281 gctcgccgtc gagccgctgc gacggtccct caagcttgct ggtgacgcgc cgctcatccg
    32341 cacgcgactg gccgaagcgc ttctcgctct ggaggaccca aggttctacc ccgagatcct
    32401 cgatctcatg cgcaagacga tcatcgagga cccggatcct cgggcctacc gggcgttcgc
    32461 gctcgtcaac tacaagctcg gtcggcgtcc gcaggcagag ctcgccacgg ccgaggcata
    32521 ttttttggaa ggtgacttca agcaggcgca gacatttgcc cggcgcgccc aggcaggact
    32581 tagaaatggc gagagcgatt ggcttcgcgc caacgaaatc gtgaccttca aggccgtagc
    32641 cggccagtga acagcccgct aatgtgccgc cacacatcgg ccggaccgca aacggagaaa
    32701 atcatgagct cacctggatt tggcagcttc ttcgacatgt tgacctcgaa gaagggcgcc
    32761 gccctcgctg tcccggccat cctactgttc ggtgcgacgc tggccatcgg ccagaagagc
    32821 ttcgatgcta tggcgcagga caaggctgcg ccacccgccg cgagcgccgc tgcgccagca
    32881 acgccgggtg ccccggccgc acctgcgggc acttccagcg ccggcgttga ccgcaaggag
    32941 gtcgagaaga tcatccgcga gtatctgatc gccaatccgg aggtcatgat cgaagtccag
    33001 acggccctcg aggccaaaat ggagaagatc aaagcggaaa aaatgaagac gatgctcggt
    33061 caggtggctg gccggctcta tcggagcccg accgctgctg tcgccggcaa tccgagtggc
    33121 gacgtgacca tcgttgaatt cttcgactat aactgcggat actgcaaagt ctcactcgca
    33181 gcggtggcca aggtcataga aggcgacccg aaagtccgcg tcgtcttcaa agaagtgacg
    33241 ttcatcggcc gcgaaacgtc agccggcgca tctcggatcg cgctggcggc ccgcaagcag
    33301 ggcaagtatt gggaggtgca tcgcgccctc ctcgaaaaca agggcgtcgt gaacgaggcg
    33361 ctcgccctca aagtcgccga gaagttcggt ctcaacatgg accagttgaa gcgtgatgca
    33421 gcgtctgcgg agatcaagaa ggagctcgac gacgccacgg cccttgccga atcgctcggc
    33481 atcgatggca cgcccttctt cctggttggc gatcgcgtga tttcgggcgc cggcgagagt
    33541 ctcgccaact ccctgcaatc tctggtggcg gacgtgcgca agaccggttg caaggtgtgc
    33601 tgagccgatg aggacgaggg gaccgtgctg accacgcggc gtggtcaccg gggtccctcg
    33661 ctccatccgc ctttgacggc gatcgaagaa agctgctaga gggctctcac gagccctttt
    33721 gcatgtgcgc agtcgagtga gtgcgccgca atagagctgc gccagtcgtc atcgattgtg
    33781 cctggacgcc ggcctcccgt cgttcgggcg aaattcctgt tcagagcgtg tgctgccaag
    33841 gttcacgttg atgcggacaa aactgatgcc caaggactcc aaggacaagg acgcggccga
    33901 caagggtctc attcgagagc tcgccgagtt gctcgtcgag accggtcttt ccgagatcga
    33961 ggtggagcgc gagggcctga aaatccgcgt cgcgcgctcg ctgaccgtgg ccgcaacggt
    34021 gcccatgccg atgatgccgg cgcccttgcc cggcacggca gcgtcctcgg cgccgcgtgg
    34081 cgatgctcta ccagcagctg gcgctcctgc ggcggctgat ccaacgtccc accccggttg
    34141 cgtcaagtcg cccatggtcg ggaccgccta ccgcgcgcct gagcccggcg cctccccttt
    34201 cgtcgacgtc ggcacccgcg tcaaccaggg gcaaacgctc ttgatcatcg aagccatgaa
    34261 gacgatgaac cacatcccgg cgccacgctc gggcacggtg cgcgaggtct tcatcgagaa
    34321 cggccagccg gtcgagtacg gcgaggcgtt gctcattatc gaatagccgt tcaggcgccg
    34381 gccgcacagc cgcgttgccg ccgatcgccc ccgaccttcc gctcgcgtcc aggatcccga
    34441 tgttcgacaa ggtgctcatt gccaaccgtg gcgagatcgc gttgcgtgtg cagcgggcct
    34501 gcaaggagct tggcatcaag acagtcgccg tacactcgac ggccgatgcc gaggcgatgc
    34561 atgtgagact cgccgacgaa agtgtctgca tcgggccgcc acaggccgcc gagagctacc
    34621 tcaacattcc gcgcctcctc gccgcctgcg agattaccgg cgccgacgcc atccatcccg
    34681 gctacggctt cctctccgag aacgcgcgct tcgccgagat cctcgaggag cacaacctca
    34741 ccttcatcgg cccgacggct gcgcatatcc gcacgatggg cgacaagatc gaggcgaagg
    34801 tcacggcgaa gcgcctcggc attccctgcg tcccgggctc ggacggcgcc atcaccaacg
    34861 agatgcaggc gcagcgcgtc gcccgcgaga tgggcttccc tgtgctggtg aaggccgcgg
    34921 ccggcggcgg tgggcggggc atgaaagtcg cccgctcggc ggaggagctc gatctcgcca
    34981 tcaagaccgc gcgcgcggag gccaaggccg ccttcggcga cgacagcgtc tacctcgaga
    35041 agtacctgac ccacccacgc cacatcgagg tgcaggtgtt cggtgacggc aagggcaacg
    35101 cgatccatct cggcgagcgg gactgctcgc tgcagcggcg ccaccagaag gtgtgggagg
    35161 aggcaccttc gccggccctg aactccgaga accgcaagaa aatcggccag atcgtttcca
    35221 aggccatgca gaagctcgca taccggggcg ccggcacgat cgagttcctc tacgaggacg
    35281 gcgagttcta tttcatcgag atgaacacgc gcctgcaggt cgagcatccg gtgaccgaga
    35341 tgatcaccgg cctcgatctc gtgctcgagc agatccgcgt ggcatccggc ggcacactca
    35401 gcgtcacgca ggacgaggtg accttctcgg gccacgccat cgaatgccgg atctgtgccg
    35461 agaacccacg caccttccgc ccgtcaccgg gcaagatcac ctattggcat ccgccgggcg
    35521 gtctcggggt gcgcgtcgat tcgggcgtct accagggcta caagatccca ccctactatg
    35581 acagtctcat cggcaagctc atcgtgcacg gcaagaaccg caacgagtgc ctgatgcggc
    35641 tcaagcgggc cttgtccgag ttcgtcatcg acggcatcga gaccacgatc ccgctgttca
    35701 tggacctcat tcagcatccc gacatccaga acggccttta cgacatccac tggctcgagc
    35761 gctatctcgc cgacaaggcc tgagttcagc tgacctgctt caacgcatcc gcagccgcca
    35821 tctgctcgag tcgcgcgcgg acgacagcga attcgtcgac gatcgttacg gctaaggccg
    35881 aagcgcgcac ctcatctttc gcattcgaag cgccctcgag atccttgcag agcgcagaca
    35941 gccgctgtgc cccgaggttc gcgctcgatg acttcaagct gtgcgcgacc atcttcaggc
    36001 tcagcatgtc ctttgcgtca ttggcagtct tcaggtccga gatcgccttc ggcgcgtagt
    36061 cgagatatgt ccggatcaag cgcctgagaa gctccggccg gtgcgtgcgc agcccctcga
    36121 gaaccttttc ctcaatcggc gccggccctg gcacaacggg tgtcggctgt gaaaccggac
    36181 tggaaggcgc tggcgcggca ggccctggat cagtgatcgc ggccgtggcc ggcccggcga
    36241 tggctgcaac agcggggccc gttgccgggg ctggcgcctc ctcgctcacg acggcgatgg
    36301 cggcctccga cgtcgccttg ctcgacaatg cctccgcgcg cgccccgcga gaccttgtca
    36361 gccagatgcc gagcatcgag acgaggcgct cctcgtcgaa cggtttcgag atatagtcgt
    36421 tcattccagc ccccaggcac ttctcgcggt cctcggcgta ggcattcgcc gtcatggcga
    36481 tgatgggcgt cggtgccaga cacgtgcggc gctcgatctc gcggatgcgc gctgtcgccg
    36541 tcagcccatc catttcgggc atctgaacgt ccatcagcac gaggtcgtag cgggcgtgct
    36601 cgatggcgac gacagcctcg acgccgttgt tggcaatgtc gacggagcag ccgtagcttt
    36661 cgaggaactc gcgtgcgacc tcaacgttga cggggttgtc ctcggcgagc agcacgtggg
    36721 cggcgaaacg cggtgcggct gactcgcgcc gtgccagtcg cggcagcgcc accttgcccg
    36781 cgacgatgcg acagacctcg tcgaggaggt caccccggcg cagcggcttc gtcaggagcg
    36841 catcgacacc gagctcgcgc gccttggaga cgtctccctt ccagctgagt gacgtcaaaa
    36901 ggatgatctt cgttgccgcg atctcggggg tggcgcgaat ggctccgaca agctcgatgc
    36961 cgtcgacgcc aggcatcacc acatcgagaa gcgcaatgca gaagggtgtg cctgcccggc
    37021 cagccttttc gagcgcagcg atcgcggccc cggccgaggc gacggcgaca acctccgcac
    37081 cctgtcccga caggtaattg acgacaatct cccgattggc ctcgcggtcg tcggcgacaa
    37141 ggatgcgccg ccccacgagc tgcgtccaat ccgggcgcaa ctccggcgca tcggctgcga
    37201 cctgcagcga gaggtcgagc gtcacgatcg tacctttgcc gaactcgctg tcgaccgaca
    37261 gcttgccgcc catcaggcga gcgaggtgga gggagatcga gaggccgagc cccgtgcctc
    37321 cgaagcgccg gctgatcgag gaatcggcct gggtaaaggg cgagaagagc ttggcaacga
    37381 tggccttgtc gatgccgatg ccggtatcac gcacctcgag acgcacatgc gtgccgcgct
    37441 ccgagccacc gtcacgcgtc aagcggatcg acacctcacc gtcgttcgtg aacttgatcg
    37501 cattgccgat caggttcacg cagatctggc gtaggcggcc cgggtcacct gcgaccgtcg
    37561 tcggcacatc gtcggcgatg aacagagaaa gctcgagctc cttgcgccgc gcctcctccg
    37621 cgaagaggtc gacggcagtc tcgatcgtat agcgcaggtc gaactcgcga tgatcgagct
    37681 cgagcttgcc ggcctcgatc cgcgacaagt cgaggatatc gttgatgatc gtgagcagcg
    37741 tccgcgccga ctgactgatc gtgccgacga ggcgtcgctg gcggtcgttg agttcggtcc
    37801 gcatgaggag atcggtcatt ccgaacacac cattcatcgg cgtacggatc tcatggctca
    37861 tgttggcgag gaactcggac tttgcccgcg tcgcgccttc gagctttctg ttggcgtcga
    37921 cgagttgttc ggcgatgcgc tcgcgcgcgc cacgctcgac gacgagggcc gtgaggttcg
    37981 caataccggc accgaagagg cggtggtccg ccgtccaggc gagcctgcgc ccgatacttg
    38041 cggtcgtaat gaagcccgcg atatcgccgt tcgccatgat cggcacgtgc atcacgctgg
    38101 tggcgccgat ggcatcgaac accagactct ggagatgcgc gagctcgtgg ttctcggacg
    38161 tgtcgtcgat tgtgacaatc cgatgcgccg aggcctggat catgagcgcg gcgaactcgg
    38221 tgttctccga gtacttcggc gcaacgaact cgcctcgcac tgcgtcatag accgacgatg
    38281 ccgcaaagcg cgtgcgcgtg tcgtcgagca gccagacccc accgcgatcc gtgcccatgt
    38341 tgagcgtcat gctcttggcg atggtttcga tcgccgtccg gatgtcgccg aagcggattg
    38401 caggatcgcg catgagcgcg ccgatcgtcg cctgctgcgc aaggaggccg tcggtgcggc
    38461 tgctgttctc gatctcgagc gcctcgcggt cggtgcgctc gagcaccatg tgagcgagac
    38521 tcgcgatcga gctcgcgaag acctgctgct cggtcgtcca tggccaccgc tcgtcgctgc
    38581 ggccatacga gatgtagccg gcgagaccgc cgttgcgcat gatcggcacg aagatgatcg
    38641 cggcgatgcc gaacggcttc aattgactct ggtagaattt acgcgtcgcg ggatgcgtgg
    38701 cggcactctc gatgatgagc ggctggccgt tttcgatggc ggcaacggca ctcgcgaggt
    38761 ccgcaatggg aacgtcgtcg ccgcgcgagt gggccctttc ggcggccctg tagcgcgaca
    38821 cgcaatgcac accctgtcga tccttgacga acatgcggat cgtgacgcta tcgacctcca
    38881 gcgccctcgc cgccgccttg gtgagggtgt cgaaggccgt gagcagcggc gcactggtga
    38941 gcgccttgcc gcgcacgagc tcgttcagcg tcgcctgctg gttgacgagc gcaagggtgc
    39001 gacgcgcgct ttcggtctgc agcatctcgc gatcccgccg ctcgatgacg ctggcaacga
    39061 ggttcgcgat cgatgccgcg aagacctgcc ggtcgggcgt ccactccgcc ttcacattct
    39121 cgctctcgaa gaacacgacg ccgatcggcc gcccgtcgcg aatgatcggc acaccgagga
    39181 gcgagacgac atggcctgga ccgagccaga cctcgacgag ctctcgcgtc gccggattgg
    39241 tgcggacatc ctcgacggtc agaacctcgc cgctctcgat ggcggcgagg agcttcgggt
    39301 tggcctcccg cgtcaattcg ttgggccaca cggacgcctg ctccggtggc tgttcaccct
    39361 cgatgcgacg cagaaccgat ccgtcctgat cgacgatcca cgccgaggct cccgtgacgc
    39421 cgagggtcac cgcgctcgca ttggtgagat gctggatcgc cccgcgccaa tccccgcgcg
    39481 acacgctgtt ggatcgaatg agctcattca gaacgagctg ctgcatgagc agatcctcgc
    39541 gcttgcggat gagctcgcct tccgccgcct tcttctgctc gatctccctg gcgagccgct
    39601 cgctggtcgc gcgcgcggcc tcctcctgct cacggagcgc ggcggtgcgc tcggccacgc
    39661 gctgctcgag cgagctattg agcttcgtta gctccccata ctggtcctcg ataatgagat
    39721 cggcacgtcg caccacggcg aagagcacga ggtagaggag gctgaaggcc gcgacggcga
    39781 aaaggccgac gaatagcgta ttgcgcttca gttgctggat ctcggtgctg atatccttgt
    39841 agacctcgaa aacagtgacg agtttgccgt tctccacaac cgggacatac gtctcggcca
    39901 cctccacgtt gcgcaccacg ccgcgcacgc cattgaaaga ctttcgaaac gaaaacctcg
    39961 agatcggcaa tagggtcttc tggactgcca cgtggtttgc gtcagttgat ctgtcctctc
    40021 caatctggcc atgctcggtc gagtagatcg tcactccgtc ggcagcatag atcttgatct
    40081 tcagcacatc gatgccggaa atggcctgca gcacgtcacg atcgagagcg gtgatctcgg
    40141 ggccttgcga agcgttggcc gaatgggcac gcctagcctt cgtgaggaaa tcgcgatggc
    40201 ttccccagac cgaattggca atggctttgg tgagttcggc gctgttccgc tcggtcgtca
    40261 gcaggatggt gcgcgtctgc tgccatttga acaggaggac gagcaccgcc gtcgccgcga
    40321 cgatgatcac cgccgacgac agcgagaaat accgcagtag cttgaacatg cccgtcccct
    40381 agcacgacac tgcccaccga aaagcgtgcc gagggccaaa ggatacttac gatctgctaa
    40441 caccggatga agccttggtt atcagaatat tgacaatgca taataatttc acgtcctatc
    40501 aagaccttcc tgccgccggg acgaagcggc gcgtcggctc caactcgcgg tcgtcgtgct
    40561 cgaggagcgc gatcatgcgc tggcgcgcgg cgcccgcatc tatnnnnnnn nnngccagtg
    40621 ccgggcgatg tccgcgtcga ggagccgcgc tgccaggtcg gacgcaagcc gctctgcgcc
    40681 gatcgccagc cccggaatat cgccggagag cggcccctgg ctcacagtcg cgccccaatt
    40741 gaaaatgtgg acgttcccga gccatgtcgc cttgctgtgc ggcgccggct gcagcgcgaa
    40801 ggcggcacca agatagggaa atcgcgacgt ctcctcgtgg tctgcagcgc tatcgggcgg
    40861 aacgcggtcg cgccacaacg cgatttcgcc agcatgaggc gacaagagcc cgttcaaagt
    40921 gagatcaacg tcgaagccgg tggcaaggat tgcggcatcg acggacgtac cttcaccgcc
    40981 gacgtcgata aagacctctg tccgtccgag cctgatcgac cgccaaccct gaccgaaagc
    41041 gatcgagaaa ttctcatgcg aatcgcagcg caaaacagat tcgtagggcg gcggaacctg
    41101 ctcggaaaat atatagctga agaatttcca acgcgtcgca tcgtcaagcg cggcaacgcc
    41161 gcgcaggaat cccggaaacg aggcgccctt cgatttgttg atctggggga gctgcgcgcg
    41221 gcgacaatag aggacgaccg cgcctgcgcc ggcctcgagc gcagccgccg cgcagtcgaa
    41281 ggccgaggcc ccggcgccga gcacggcgac gcgcttcccc ctgagcgagg cgaagtcgat
    41341 ctcctcggcc gagtgggaaa tgcggccacg ccgcgctggt gactgccaat cgagccccgc
    41401 aatcaccggt agcctctggc cacccgagcc gtcccggccg gtcgcgagca caacgtgccg
    41461 cgccagaagc cgctcctgtc cgacagctga tgatgcctcg acctcaatca accgaccggc
    41521 gggccggatc actgcaacct ccacgccgtt ctcgaccggg atgccgaccg tgtcgcgcac
    41581 aaacagcaga tagtcgcgcc agtcgatccg cccggccttg tgcagtgagt gccatgcagc
    41641 ctccccgcag ccagactcgt accaggccct aaacgtgagc gaaggcacgc cgagatcggg
    41701 gccggtgagc tgcttcggcg agcgaaggat ctccatgcgc gcataggtgc cccacggccc
    41761 ctcctctccg cgcgccgctt tgtcgacgat cctgaaattc cggatgccgg cccgcatcaa
    41821 cgcgaatgcg gccgtctgcc cgcacatgcc gccgccgatg atgagcacgt cgagcacggg
    41881 gcggccgtcc gccgaacagc ttgtcgggac ccagttcggc gcggggaagt tcagcatctc
    41941 gaggtcgcgc cgggcggcgg ttctcagccg gtcgatcgct gcggggtctc gctcgggctt
    42001 ggcggctgac cagatcccgt ccattctctc gctcatgcgt cactcatcga gggtcagatg
    42061 atcgagctcg actgctacta cagcctgtcc tcgccgtggg cctatttcgc gggcccccgc
    42121 ctcgaggaca tcgtgcgtcg ccatcatgtc aagctcaacc tcaagccgtt cgacttccag
    42181 gcggtcgtgc cttcgaccgg tggagttccg ttgcgcaccc gccctgcgcc gcggcggcgc
    42241 tatcacgatg ccgagctcga ccgctggcgc caatatctcg gcatgcccct gaacctcaag
    42301 ccccgacact accctgaggc cgtgaccgat cctgactgga acaagtatcc aggctgggca
    42361 gtaatcgcgg cgagcgagga cggcaagccc gcgatgcggc tctcgcaggc gctgttgcgt
    42421 gcgctctggg ccgaggagcg cgacatcacc gatccggccg tccgccgcgc cgtcgccgac
    42481 gagaacggct acgacggcgc agccgtcgtc gcggcggagc ggagcgccgt ggtgcaggaa
    42541 aagtacaggc agttcacgga tgaggcgctg cggctcggcg tgttcggcgc gccgacctac
    42601 gtcgtcgatg gcgaactctt ctggggtcag gaccgcctcg gtttcgtcga ccgcaaactc
    42661 gaccggatga gggcggacgg aaagtcctga gccgcacgtc accctatctg ccgctgagtg
    42721 cgacgagtac gacgcccggc acgacgagcg caatcgtcgc ggccgtccgc aggctgaagg
    42781 cctcgttgcg gaagacgagc aggccgagta gcagcgtgaa aacgggcgct gcagagacga
    42841 tcggcacgac tgtcacgacg ctacctgccg ccagcgccga gttcaggaag tgcagcgagc
    42901 ttcccgcggc gatcccggcc atcacgaacc atttgtgatc ggacatgcgg cccgtgatgc
    42961 gctggccctg cccgaagaac gccgccgcga ccagcaccag ggagaccgta ttgccgacaa
    43021 gcaccgcgaa tgaggggctc ggcacgtctc cgaggccgag tttcgtcccg acgtggcccg
    43081 cggcgcgaat gaatgcggca ccgaagggaa ggacgagcgc ccagagcggc cagccccgcg
    43141 caacgccgtc gggccgcatc gtcgccacca cggcgccggc gacgacggcg agcgtgccga
    43201 tcgtggtcgc aagcgtgagg cgctcgttca ggatgaggat cgcaagccca gcgccgaaga
    43261 tcggcgcgga ggccgtgagc gcgctcgtca aggtcggtcc catgcgcgcg atactcgaga
    43321 gcgcgagcca ggacgagagc gacggtcgga agaggccgac cacggcgaag cagataaagc
    43381 cccacgtcca ccagtagcca ccttcgagga agaacggcgc agcgagccag tacatcgcgg
    43441 cgcccgtcgc gatgttgacg agcgagcccg agcggacgtc ggcgcccttc aagccgatgc
    43501 tctggaaatg gttggtgagt gcaaagagga atgccgagat cagggcaaac agaacgggcg
    43561 tcgacatcgg ggcggaacct cgagctcggt cacggcaaat catcgatctg aaggctacaa
    43621 cagcttccgc tccggagcac gaccggtcgc gcaaacgccg tccgcgcaac atgaatagtg
    43681 gctgtcggat gggttcagga tgggttcaag ccggcggcgg cattctcccc ctcgatgagc
    43741 ggccgacggg aatcgccgct cgacaggagg agcagatgaa gttgaagacc ctcacgcttg
    43801 ccgccgcgct cgtcgtcgca gccaccggcg tcgccagcgc ccagagcccc ggccgcggtc
    43861 atggcccggg ttatggctac ggcccaggcc actctggcgg cgacatcgac tggaaccagt
    43921 ggcagcagaa gcggcgcatc gagatgggcc gcagcgacgg ctcgctctcc ccgcacgagt
    43981 accgtgcgct catcgaggag cagcgccgca tccaggacct cgaagctcgc ttcaagcgcg
    44041 acggtcgcct ctcggccgat gagcttgcca ccctgcgccg tgcacagagc gccgccagcc
    44101 gaaatattta cgaggagcgc cacgaccgag acacggcgcg ccgcggctgg ttcggctggg
    44161 gccggcgctg acccgaagcg cccttccgga cccgactgcc agaatcaagg agggcggcca
    44221 ctggccgccc ttcacgcatt ctcatgcggc tcgcatcagc gcgagtagaa ctcgacgacg
    44281 agattgggct ccatgcggac cgggaatggc acgtcgccaa ggcccggaac gcgcaccatc
    44341 ttggcggtca tcttgttgtg gtcgacctcg aggtaatctg gaaggtcgcg ctcgccgctc
    44401 ttcgtcgcct cgagaacgag ggcgagctgg cgcgacttct cgcgcacctc gacgacgtcg
    44461 ccgacgcgga ggcgatagct cgagatgtcg accttcttgc cgttcacggt cacgtggccg
    44521 tggctgacga actggcgggc agcgaacacc gtcggcacga acttcgcacg gtagacgacg
    44581 gtatcgagcc gtcgctcgag caggccgatc atgtgctccg aggtcgagcc cttgaaacgg
    44641 cgcgcctcgg catagatgcg cttgaactgg cgctcgccga tgttgccgta atagcccttg
    44701 agcttctgct tggcgcgcaa ctgcatgccg tagtcggtga gcttgcccgt ccgccggtca
    44761 ccatgctggc ccgggcgctt ctcagtcttg ttgagcgggc tcttcggccg gccccagatg
    44821 ttctcgccaa ggcggcgatc aatcttgtgc ttggcgctga tgcgcttcgt catgtttgga
    44881 tccgtctttt gctatgcacg ggatggagcg ctatctcctc tgccgcacga agcgcgaggc
    44941 gcctcatcca gccgacagat gacgaatgac cgccatcacg aaagcagtac gtttcgccgg
    45001 cacgcgggcg cgccggacgc aggtgtgctt ctaggcgcgc ctcagcgact ggtcaaccgg
    45061 caatccgcgc gagcattggc cgctctgcgg ccagcggggc tggccccgcg cccgctggga
    45121 ggcggagcct cccaggcgca ccaccttttg gttggttatg tcgcaatcgg gtgcctcgtc
    45181 atgtcgagaa gcgggaggtt cggagggtgt ccctccgacg ggagcgcgag ggctggccct
    45241 cgctcgcgaa gcgatctcgc cgcgcttgac agcggcgccc gcgggtgcgt tatgccgccg
    45301 cagacaatac ccgatcaaag catggacgcg gttcactccg aggagcaccc atggcaatcg
    45361 ctttcatctt tccgggccaa gggagccagg cggtcggcat gggtaaggag ctcgccgcgg
    45421 cgtttccggc tgcccgcgcc gtcttcgagg aggtcgacgc ggctctcggc cagaacctct
    45481 ctaagctgat gttcgaggga ccgcaggatc agctgaccct cactgagaat gcgcagcccg
    45541 cgctgattgc cgtatcgctc gccgtcgcgc gcgtgctcga agccgagaag ggtatctccg
    45601 tcgccaaggc agcgaagctc gtcgccggcc acagcctcgg cgaatattcc gctctcgcag
    45661 cggccggcgc cttcaagctc gcggatgcgg cgcggctcct caagttgcgc ggccagtcga
    45721 tgcagaaggc tgtgccggta ggggtcggtg cgatgtcggc gctgctcggc gtcggcctcg
    45781 acgtcgcccg ccaagtcgcc gccgcagcgg cccaaggtga atgctgcgag atcgccaacg
    45841 acaatgagcc gacgcaggtc gtgctctcgg gtcacaagac ggcaatcgat cgcatccccg
    45901 aggcggggaa ggccttcggt gtccgtcgcg cgttgccgct ccccgtgtcc gcgcctttcc
    45961 attgcgccct gatgcagccc gctgccgacg ccatggcaga ggcgctctcc aaggtcgaga
    46021 tcaaggcgcc gtccgtgcct gtggttgcga atgtgctggc tgccccgatc agcgacccgg
    46081 cggagatccg ccgccgcctc gtcgagcagg tgaccggcac cgtgcgttgg cgcgagtgcg
    46141 tactcgccat gtctgctgcg ggcgtcacct cgtaccacga gctcggcgcc ggcaaggtgc
    46201 tcgccggcct cgtaaagcgt atcgacaaag ccgccaccgc cacctcgatc ggcacccccg
    46261 cagacatcga agcagccgcg gcgggcttca aatcctgacg accaactttc ccaaccgtac
    46321 acaaggaatc tcaagatgtt ctctctctcc ggcaagggtg cgctcgtcac gggtgcgacg
    46381 ggcgggctcg gcggcgcgat tgcgcgcgtc tttcacaagg ccggcgccaa cgtcgcgatc
    46441 tcggggacca aggccgatcg gctcgaggcg ctcgccgccg agctcaagga gcgagtgcac
    46501 atcgtcccct gcaatctcgc ggatcgtgcg gccgtcgcca agctcgccgg cgaagcggag
    46561 gccaagctcg gccagctcga catcctcgtg aacaatgccg gcatcacccg cgacaatctc
    46621 ttcatgatga tgaaggacga ggagtgggac caggtgctcg ccgtaaacct cacctcggcc
    46681 ttcacgctgt cgcgctccgt gctgcgtaac atgatgcgcc gccgctttgg ccgcattatc
    46741 aacatctcat cggtctccgg catcatcggc aatccgggcc agggcaacta cgcggccgcc
    46801 aaggccggca tgatcggcat gacgaagtcg ctcgcccgcg agatcgcctc gcgcaacatc
    46861 acggcgaatt gcatcgcgcc gggcttcatc acgacctcga tgaccgaggg cctcaacgag
    46921 aagatcgtcg aggaagcgaa aaagcacatc ccgatggcca agttcggcac gcccgacgac
    46981 atcgccgccg ccgccctcta tctcgccagc agcgaggcgc aatacgtcac cggccagacg
    47041 ctgcacgtga acggcggcat ggcgatggtg tagacgctaa tcacttttcg gagcagaaga
    47101 tgcgcgaggc cctgatcgtc gtgcggccac gggacacgtc ttggtgctcg ccaaagccga
    47161 tggcgtgtgt taacgagccg ctcgtcgagc gctcttgggg aaaagcctcc gccggggacg
    47221 gctacgatgt gccctccgag agcaactcgg attagtttcg tgtcgcgcgg ccactggggg
    47281 cgtcccctcg gcaaggtgcg gcattaatgg gagcacctcg cgataagcgg ggacactggg
    47341 attggtccag tcaaaaggga cgaagcatga gcgacatcaa ggaccgggtt aagaagatcg
    47401 tcgtcgacca gctcggcgtc gaggccgaga aggtcacgga agcggcgagc ttcatcgagg
    47461 acctcggcgc cgacagcctc gacatcgtcg agcttgtgat ggccttcgag gaggagttcg
    47521 gctgcgagat cccggacgat gccgccgaac agatccagac cgtcggcgat gccgtgaagt
    47581 tcctcgaaaa atcgagcaag gccgcctgat cggtcggccc atgtaagttg gcgagggccg
    47641 ctgcagtccc ttcagagcct gatccgctga acggcacgcg cggccggacc ggctgatcgg
    47701 gctctctgcg ttgggagggc acgcaagccc gatcgcgccg tgctccgacg ccacccgaaa
    47761 gctccgcaac atgaccgctc gcaccccgca gaaattctat gccccgctcg ccgtcggcgc
    47821 gccggcccca gtgcgccagc tccccgtgcg gctcgagcgc atgatccact tcctgccccc
    47881 ccacaacgaa aagatccgcg cgcgcctccc cgagctcgtc ggccaggtcg atgtcgtgct
    47941 cgcgaacctc gaggacgcga ttcccgccga acagaaggcg gctgctcgcg ccggcgccat
    48001 cgagatcgcg cgcaagacgg acttcggctc gaccggcttc tgggtgcgca tcaattgcct
    48061 gaactcgccc tgggcgctcg acgacatcat ggagctcgta cccgctgccg gtgccaagct
    48121 cgacgtcgtc atgctgccga aggtcgaggg cccgtgggac gtgcactacc ttgaccagct
    48181 gctcgcccag ctcgaggcgc gccacaagat cgggaagccc attcttatcc acgcaattct
    48241 cgagacggcg gagggtgtga agaacgtcga ggcgatcgcc tcggcctctc cccgcatgca
    48301 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcctatggg ctcaagccct tctacggccc
    48481 attcggcgac ttctcggatg cggcagcctg cgaagcgcag ttccgcaatg ccttcctgca
    48541 gggctgcctt ggcgcctggt cgctgcaccc gactcagatc gacattgcca agcgcgtgtt
    48601 ctcgccggac gtcgccgagg tgctgttcgc gaagaagatc attgcggcca tgccggacgg
    48661 cacaggcgct gccattgtcg agggcaagtt ccaggacgac gccacctgga agcaggccaa
    48721 ggtgatcgtc gacctcgccc gcctcgtggc cgcccgtgat ccggcgatgg caaaggccta
    48781 cgggatgtga gtacgcgcga tcagcaccgt cattggtcgc agcgcctgcc cttctattac
    48841 ggctggctca tcgtcgcggt ggcgttcgtg accatggcga tcggcgtcac ggcgcggacc
    48901 gcattctcgt tgctgctgcc gccgttgatc gacgagttca agtgggatcg cggcctcgca
    48961 gccggtgcct tctcgttcgg ctttctcgtg tccgccgtcc tcagtccgat cgtcggccgg
    49021 atcatcgaca cacgcggacc acgcctcgtg atccaaatgg gcgttgcgtt gatgtcgacc
    49081 ggacttctgc tggcgccgtg ggtgacaacg ccctggcagc tctacgccac cctcggcgtc
    49141 gctgtcggag gcggcgccaa catgatgacc tacaccgccc attcccaatt cctgccgaac
    49201 tggttccaac gccgacgcgg gctcgcaatc agcatcgcat ttgccggtgc cggcgcggga
    49261 gcgatcgtcc ttctaccctg gctgcagacg gtgatcctga cgcgcggatg ggcccagtcc
    49321 tgcgtggtca tgggcctcct cgtgctgctg gttgtcgggc cgctgagcta tctggtccag
    49381 aaggcgccgg ccaatgtcgg gcagtacccg gatggcgatg ccgctcctgc aggctccgtc
    49441 acgaagcgca ttgcgacggt ggtcgatgag gcgtgggcac aaaccgagtg gacgctcgcg
    49501 aaggccgccc gaaccgcacg cttctggtgg atcgtctgcg cctatttcct ggcgctgatc
    49561 gcctggtacg ctgttcaggt gcaccagacg aagtacctga ttgagatcgg cttttcgccg
    49621 gtcgtcgcgg cgtgggcgct cggcgcggtc agcgtcgtcg ccatcccggg gcagatcctg
    49681 ctcggcggcc tctccgaccg gatcggccgc gagtggatct ggacggccgg gtgcctcggc
    49741 ttcgcgaccg cgtacgtggc actgatcgcg ctcgagcagg agcgctcgac cctgctgctc
    49801 tacaccatga tctttgcgca aggcttcttc ggctacgcgc tgacttccgt gatgggaccg
    49861 attgttgccg aagtgttcga ggggccgcac tacggctcga tcttcggcgc catcacgatc
    49921 gcgctcatcg gcggcggtgc cgcaggaccc tggatcgccg gcgccgttca cgatgccacc
    49981 ggcagctacc ggctcgcgtt catcggcatc atcgccggct gcctgctgtc ggtgatcgcc
    50041 atctggatcg ccgggccgcg caaagtgcgg ctcgtcgccg ggcgcgcccg gaaatccgga
    50101 tgagtgtccc ttcgcaggcg gccgatggcg cgatagcata ggtagaaact ac
//
LOCUS       DAORNH010000004        46041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_591_length_46041_cov_8.917866, whole genome shotgun sequence.
ACCESSION   DAORNH010000004 DAORNH010000000
VERSION     DAORNH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 46041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46041
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_591_length_46041_cov_8.917866"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK264_00760"
     CDS             complement(<1..235)
                     /locus_tag="PK264_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
                     /protein_id="HRD74461.1"
                     /translation="MATIGTFKKTGNNEFSGEIVTLSVQAKNVRIVPDTRATGENAPS
                     HRVYVGRVEIGAAWSKRSNEGRDYLGLKLDDPSF"
     gene            complement(806..1246)
                     /locus_tag="PK264_00765"
     CDS             complement(806..1246)
                     /locus_tag="PK264_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74462.1"
                     /translation="MIILAILGSLAAIGVLCWLLFKLAVFALPAFAGVTIGVWAHQTG
                     AGALGAVAIGVVAAGAILGFGQLLLAFVRPMWLKLLVALAFVAPAAFAGYHATHGIVK
                     HTMPSETWQLVFSVIGAVAVGITAFVRITTMASPGPSRQDIATA"
     gene            complement(1388..1552)
                     /locus_tag="PK264_00770"
     CDS             complement(1388..1552)
                     /locus_tag="PK264_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74463.1"
                     /translation="MSLTDAQAFAFSLATTLMVAIVIFRAGDGTLSVVPSNEFDGDAE
                     IVAEIDPFAS"
     gene            2043..3875
                     /locus_tag="PK264_00775"
     CDS             2043..3875
                     /locus_tag="PK264_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD74464.1"
                     /translation="MQVVRLKISGFRGVRSADIVLGRHAVLVGPNNSGKTTVIEALAL
                     LFGRDRLVRRLTEHDFHGSAPDETARILCIATVTGFTPNDPHHHLSWFNPERGVEKWF
                     DPKAKTLSAAPDARHTDLAVQIGFAARFDLDELEAETLRFFVDDEATLGDPFAEDAHL
                     RTIHTKVLQELGFFLVPASRTWDRWISFSSELFRRVVATRGDMPAQAVRAERQRLWTP
                     PDGARLEDQPGLSEIVGAANDELRALMASAPRLQLRLTATDSDSVLESVVPHFVQGTG
                     PTLPSQRQGTGLVSLQSLLLLMQFGKARAETGQSFILAVEEPELHIQPSQQKRLVNRL
                     NALCNQTIVTTHSPIVAAMFPAPDTLFIETRDGALSAKPLMDAVPAQPTNHQQHLLYA
                     WRQKLVAALMHECVLIPEGVSDVAWLEALQTALEQHQGWDNAQANTPLLSTFVGIVPT
                     IDAKIADTFALVGAVHARPCILVDGDNDGRGYFDAVKGSNPPPRCVVFWPQGWAMEHV
                     VSWIAGADEAAMLTALGTALGAPFADREALTAHLLTQKSYAPTHESAVVTLMANAACR
                     ARAAQLLNALCDVLRDPANANTPLFARIPADSTADMHVFRFVPT"
     gene            3872..5200
                     /locus_tag="PK264_00780"
     CDS             3872..5200
                     /locus_tag="PK264_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HRD74465.1"
                     /translation="MTFDFFEGAAGTGKTHNLVARAGEIVGDGALGEGHKLLALTFMN
                     GARRRLDARLGENPAFRRRFDCQTFDVFARTLAARRRSLITPAMQEQAIALNEFDGPC
                     ALAAGLLDREPVRQWVARTFPLVLVDEAQDLDEHRMGILQGLSQSCWVVAGADAFQCL
                     HDGRDTAHLMAWLEGAGHTHHLTQPRRTNQQGLLAAALAVREGRDIKAVLAANTFNNR
                     TTWNGAGFRLLETSATQNNTGLLAWAIANDFSQRQGAVAVLTPDAGNAIIRAALATIQ
                     TRQWTRNNGATFGPYPHTWDRHDNEEADALLGNIALPEAASCADLRALLTPLAGHAAI
                     AQAISRMDRLRRAHGKAAFTAAQVTEFVRDSVRNRSRLGFRQQHGHLAMTIQRAKNRE
                     FPNVIVLWPHTATGSAEHLRRLLYNAITRARNHCTVIVLGQGRLNAPPFA"
     gene            complement(5278..6201)
                     /locus_tag="PK264_00785"
     CDS             complement(5278..6201)
                     /locus_tag="PK264_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HRD74466.1"
                     /translation="MSEHDDHEPQHTSSPTDHALTELQLYGYRPYEDEPDPRPLPEGN
                     AVAGAIADIFDALIATLGDTRLEPDLDDLLWSTVNVFHRAAERIARDLDDNEQAQRRS
                     QREQDGTEVKSVELERLIAEGRTLVERQDAFELMRDQAGEHYERHAGKPWLPRNGSKV
                     NHRNLTSAMIDSRDFLAARKRAEQEVLLPPGPKIAFTGGLEFNDHRLIWAKLDQVHAK
                     HPDMVLMHGKSPKGAEKIASRWADHRGVPQIGFAPDWTKHGRAAPFKRNDQMLEVLPI
                     GVMVFPGTGIQDNFADKAKKLGIPVWRFGGA"
     gene            complement(6508..7569)
                     /locus_tag="PK264_00790"
     CDS             complement(6508..7569)
                     /locus_tag="PK264_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRD74467.1"
                     /translation="MVRHDASDLAQRLGRQAEAVCRHYLSSGFRQGRYWLVGDVRNTP
                     GRSMFVRLKGPETGKGAAGKWTDAATGEHGDLLDVIREACSLLDFKDVADEARRFLSL
                     PHPEPESDERRTRKPNAPTGSPEAARRLFAISQPMTRTLAETYLRKRGITALHETGSL
                     RFHPRCFYRPDEHSPTETWPAMIAAVTDLGGKLTGAHRTWLDPDGFSEATLGKAPIDT
                     PRRAMGDLLGHAVRLGAAGSVMAAGEGIETMLSLRCALPTMPMVAALSAAHLSAILFP
                     DVLRRLYIARDDDPAGDGAMAALIDRAEQAGIEAIVLSPALGDFNEDLRMLGIDALRA
                     GIRVQIAPQDVARFMELAA"
     gene            complement(7569..11903)
                     /locus_tag="PK264_00795"
     CDS             complement(7569..11903)
                     /locus_tag="PK264_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HRD74468.1"
                     /translation="MHSLSSRATAPAAPLSSVPPIDTAAATLTAARQLLPQLERGQRI
                     DAAILRSAMETAFGASDTSGVWDWKTAYDACEAATVLFLRKYGKALLRKAGSPAAALP
                     LLAKIAGLLPTHTRRSAESEAFQQFSTPLPLGLAALTAAAVTPADRVLEPSAGTGLLA
                     ILAEIAGGTLTLNELADTRAGLISSLFPAAPVTRFDAAQIDDHLDPSVTPSVVLMNPP
                     FSVMANVSGRMVDAALRHVTSALARLADDGRLVAITGANFSPDAPAWRDAFVRLQQRG
                     RVVFTAAIDGAVYAKHGTTIETRLTVIDKAPAEDPAAFPASPGIAPDVVTLIGWIAEH
                     VPPRPAVAATIAAPVASGPVPKTVRGYLARAASTPAANASLAEPEGADLSYETVDWAP
                     AESTRLTDAIYEEYGLQAIRIPGSQAHPTKLVQSAAMASVAPPKPSYRPRLPENLVTD
                     GILSDAQLETVIYAGEAHSDYLAGCWTVDDTFDLVTAAPDDAKGAVRFRRGFMLGDGT
                     GAGKGRQSAAIILDNWLRGQRKALWISKSDKLIEDAQRDWSALGMERLLVTPLSRFPQ
                     GKPVTLQEGVLFTTYATLRSDERGEKVSRVKQIVEWLGSDFDGVIIFDESHAMQNAAG
                     GKGERGDVAPSQQGRAGLRLQHALPNARIVYVSATGATTVHNLAYAQRLGLWGGEDFP
                     FATRAEFVEAIEAGGVAAMEVLARDLRSLGLYTARSLSYDGVEYELVEHQLTDEQRRI
                     YDAYAGAFAIIHNNLDAAMQAANITGETGTLNRQAKSAARSAFESTKQRFFGHLLTSM
                     KTPTLIRSIERDIEDGHACVVQIVSTGEALMERRLAEIPTEEWNDVRVDITPREYVLS
                     YLQHSFPVQLYEPFTDSEGNLSSRPVYRDGQPVESREAVARRDRLIEQLASLPPVPGA
                     LDQIVQRFGTDIVAEVTGRSRRIVRKGDRLMVENRAGSANLAETAAFMDDQKRVLVFS
                     DAGGTGRSYHAELSAKNQRLRVHYLLEPGWKADAAIQGLGRTNRTNQAQPPLFRPIAT
                     DVKAEKRFLSTIARRLDTLGAITRGQRQTGGQGLFRPEDNLESPYARVALRQLYMLIA
                     RGKVEGCSLQTFESATGLSLMDDGGLKDELPPITTFLNRLLALTIDLQGVLFTAFEQL
                     LNARIEGAITSGTYDIGLETLKAESFVVADRRTIYTHPATGAETRLLTIAQRERNRPL
                     ALGQALDHLSDPRAVLLVNERSGRPAVQIPAPSVMLDDGEIERRVRLLRPMEHHHASL
                     KVMAESHWQEADRDAFAAAWNREVAQVPEFSDSTIHIVAGLLLPIWKRLPNESTRVYR
                     LQTDEGERIIGRRVSPAWVVGALATGPSTLTAGDAFAALLDGNTILDLAEGLQLRRVR
                     VMGAHRIELSGFTDTMRDRLRAYGLFHEIISWKLRMFVPSDANGAGVLAKVLERYPVE
                     RVSEREAA"
     gene            complement(12111..13607)
                     /locus_tag="PK264_00800"
     CDS             complement(12111..13607)
                     /locus_tag="PK264_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRD74469.1"
                     /translation="MLTEKTARASDRRAVFVGLDAYEAAGGMVLRDLFQQDDGGWLED
                     IALLDNLVAAKLKAEAETIAAEGWKWIEVATDFPYGHTLGLRELDGVTPDLTAEEQAT
                     IDALNAEYQKLEAEYEDADELPDEVDARLGEIETALGQFDDRPVTYDPADIARAGVFV
                     SIGPDGRLFVDRGYVRPEDEAPAVDPGHEIDGDVASADQDGAEAGASTVQRAVITIGG
                     QPAEPEDEEDDAVRPLPDRLVMELTAHRTLALRDAVANNPHVALTALLHKLVVDTFQR
                     TATSGGCLEASVRHVFFPVQAADLKDSPSARSIAERHEAWKADLPQDDQALWVWLAAL
                     DETSRLSLLAHCVSYGVNALYEKPNPYGGNGVSQHGLERRLGQADRLAHATGLDMVEV
                     GWAPTVDNYLGRVPKRRILEAVREGAGERAAQLIDHLKKGDMAKEAERLLAETGWLPE
                     PLRLADGEEPLAGGSDAETDGDIETLPEFLSGDDDGSTVEVDLPVAAE"
     gene            complement(13962..14252)
                     /locus_tag="PK264_00805"
                     /pseudo
     CDS             complement(13962..14252)
                     /locus_tag="PK264_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
     gene            complement(14415..14939)
                     /locus_tag="PK264_00810"
     CDS             complement(14415..14939)
                     /locus_tag="PK264_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74470.1"
                     /translation="MRAPSSSRRRRHRQEAASSPAASHASAPARGSPTRWRGKYLFDN
                     AVGFLAGARHLVSSDPHSREIFLQLIGCAVELALKSYLHFAGWDDDRCRAEVRHDLVK
                     ALAAAERLGFHRRHPDVRMLTRILSPYYCCHRTRELARREPTPLSSERALRAAEGLLD
                     DVLAAVAARPRSKG"
     gene            complement(14939..15898)
                     /locus_tag="PK264_00815"
     CDS             complement(14939..15898)
                     /locus_tag="PK264_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRD74471.1"
                     /translation="MSRHATRARSGSDRTSLYDEVTGKILAELEAGRLPWVQPWGTAA
                     AKAPLAMPKNAATGRGYSGINVLILWGAVIERGFPGQSWLTFRQALSLGGNVRQGERG
                     TTVVYADRFVPDDEKQRARETGEEAQAIPFLKRFTVFNLAQCEGLPDDLAIVAPPPGP
                     GLIEPNVDALIKASGIDFRIGGDRAFYVPARDYVMVPPPQAFFEPINWHRTALHECSH
                     ASGAPHRLNRDLSGSFGTKKYAFEELVAEISAAFCCAALGIVPTVRHADYIGSWAEVL
                     REDNRAIVRAASQASKAADYLLSFLPEHDQFAVSVDTREREAA"
     gene            16232..16876
                     /locus_tag="PK264_00820"
     CDS             16232..16876
                     /locus_tag="PK264_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74472.1"
                     /translation="MPATSPIYSEPDEVSTRLASMGLTEEPLLHAARRWHLSWISFTA
                     NHPPFGIGIAAWMEAVAALREGLLPIGWTRSDEKNYALVLHPNRSMAINVATGDAGTG
                     LPTANVSNKAPKGVSTADAISVNQVQLELDLPVPDMPHMRGDEGPLTWFLLLHRAGTE
                     VRCELSLPSKISPDGRITRWQERIMLPSVPLDDSETEIVAPLGPDLDIDVKRKA"
     gene            16873..17985
                     /locus_tag="PK264_00825"
     CDS             16873..17985
                     /locus_tag="PK264_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRD74473.1"
                     /translation="MSGIDVFNPTRLTWARRRRGLTKTRLAASIGVELRSVSAYETGE
                     FNPEPDRLSVIARTLKFPQEFFFGDDIEEPAIDTASFRSLSKMTARQRDTALGSGAVA
                     MLLNEWIEARFDLPTPDLPDLGRDANPESAAAAVRQAWGLGELPIKNMVHLLESKGVR
                     VYSLAIDAVEVDAFSMWRRDRPFVFLNMLKSAEHGRFDAAHELGHLVLHRHAAPSGQE
                     AEQDANAFASALLMPPASVRAHAPRFATIDNLMRLKKAWGVSLAAMTYRLHKLDLLSE
                     WHYRKLYIEISSRGYRKKEPDGGPREVSQVLQKVFSALRTEGVSKEEIAEALRVHPTD
                     IDELVFGLALTSLDGIARRSEGGRSRPSLTLVSSKD"
     gene            17989..18900
                     /locus_tag="PK264_00830"
     CDS             17989..18900
                     /locus_tag="PK264_00830"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRD74474.1"
                     /translation="MLSLAEAQAVENIADLLYEFLPGSGNNKTAFPIAATQAGVGEFW
                     MGGSKRPAIVELLKLTLDRRRDRFTALIIAIVHQSMTWRRGKGNPLTRDEIDRLNAAL
                     PGVSFKIPDLLDPAFLKSFGEPAKAAPASSRVILSEASAQALVARLMEVSGLGPQERG
                     LRFEGFLTDVFAAFDLAPRGSFRIVGEQIDGSFRLHTETYLVEAKWHGPQIGFADLMA
                     FSGKVAGKAAWARGLFVSNSGFTQEGLEAFSRGRQTNLICVDGLDLYEVLSRRVSLIE
                     VLEEKARRAAETNRAYIPVRDLTFQAR"
     gene            18902..19435
                     /locus_tag="PK264_00835"
     CDS             18902..19435
                     /locus_tag="PK264_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74475.1"
                     /translation="MVGSGSDETTGTGKWSQPGVPHKGWRCVDIEERDEQDHLCEMCE
                     ARQVRFVHAMEHPAYDEILRVGCVCAGHMEEDLVGARKREAAYKNDRSRRVRWLQRAW
                     RISAAGNEFLNTNDGFNVVIYPKGSIWGGRVEHRPSGYQRTSKLPYGSPDEAKLAAFD
                     AMLGMKHSEPWKKKART"
     gene            complement(19771..22917)
                     /locus_tag="PK264_00840"
     CDS             complement(19771..22917)
                     /locus_tag="PK264_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD74476.1"
                     /translation="MSNERFRAAPALAPLKAFQRRTVDYVFDRLYGPDDPVHQFLVAD
                     EVGLGKTMVARGVIARMIEHLWDSVERIDILYICSNQAIAAQNLNRLNVLGRRELALP
                     TRMTLIPLQLRDQEGLDANKVNFISLTPGTTFDLRSATGVTQERALLLHLLKDHVSRP
                     RGLHNLLQVTAGVDGWNWAVDSLDLRGVDTRIIERFRRDVQADRDLFQELERVCEMFP
                     RRRDAYPWEMTQPRNSLVARLRAKLSHACVDALQPDLIIMDEFQRFRDLLHGDSDAAI
                     LARELFDYSGSDGHAARTLLLSATPYRMLTLAGDEPDEGDHYQDFLETLSFLYGRERG
                     PEVAKTLAREMRAFRGFLHGLPASHAAAVEARRTVEQRLRRVIARTERVASTVERDSM
                     VGEPPVAVSVAPADLAQAAAVSNVARALDAPEITEYWKSAPYLLNFMRHYSLKRLLEE
                     RTNTPSAALRDAILAARPAMLDHDALDAYAPLDPANGRMRAIMDDIFGAGLEQNLWIP
                     AAMPYYGSQRSQPPLTKALIFSSWSMVPDAIAALLSYEAERRMGVGESGRRYFEHHRL
                     RPLQFRQDQGRLAGLRTLLLIYPSPKLAEIADPLSVFAGQNERLSQEAMRAAIADRLE
                     PALDALRQVAAQDGGTAVAEWTAPAVIDDLLGSRAGAWLSAPGGMAALASEEGFREHV
                     AELSSAAKQRDFGALSDDLRDLLVDVALGSPAVCALRALRRIAPELAWDDPRLLSAAA
                     EVAWAFRTLFNQHDAIALLRRGAEDRYWHRVLTYGVDQNLQAVLDEYAHYLVDAEGLG
                     ARPPAERAGGVARAMATAMSIRPSQIDVDDVHVKGDALTIGKFQMRGRFAMRLADYRD
                     EEGAVARLGGVRDAFNSPFRPFVLATTSIGQEGLDFHPYCYRVYHWNLPGNPVDLEQR
                     EGRVHRFKGHAVRLNLAERQVYALRGNGHTPDDPWRQMFEQARSETTVDTDLIPYWIY
                     EGSVRVERRVPMLPFSREVTRLAWLKRSLTVYRLAFGQPRQDDLLDYLQTLAGAGMDV
                     ADLADLHIRLEPPAEPVL"
     gene            complement(22907..24763)
                     /locus_tag="PK264_00845"
     CDS             complement(22907..24763)
                     /locus_tag="PK264_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRD74477.1"
                     /translation="MLNRRSLDPEQRTLYGANLQPPAGYIFDAAVATTFSLDFETALA
                     VPVSLALFAAENREDILTHPLALLEGAERIAGRLLVFTDAGHIQAHTRPHSRLCSLLE
                     RIIVEVAAPSGGAFHPKIWALRFKPVRSDEPTRLRLLVLSRNLTRDRSWDIALTLDGV
                     VARRPQALNRPLADLIRRLPDFATAGLPEGARDLTEEIAEDLRRTDWSLPEPFQSLAF
                     AVNGLGGKQWRPEPCARLGVISPFCDDTALSMLADLAGAEKPVLIGRSDELALVPAAT
                     LGRFARVAVMDEMAATEDGEEVDATALQGLHAKAFITEMGWDTTITVGSGNATRPALL
                     SGSNIEVFATLTGKRSRVGSIEEILGEKGFGRLTRPFVPNELVAVDSALRAAEARLDA
                     ARRDICCSGLKLRCERTQSADDDSLLWRVWLIPSTPLPLAGIGGLRIWPITRGEGHGQ
                     DALERLRLGQPIDLGAMPLIDLTHFLAFHLVDGDGEVSLLFSTGLLLEGLPPERHAAI
                     LRWVIDSKDAFFRYLRLLLSELGDPFAAALAAQEGSGHGAWRMAADDAPLLEEMVRAF
                     CRGGDQLRAIERLITRLETVEPGEADPIPAEFRTLWETFRVALKVPEGAHVQ"
     gene            complement(24753..25931)
                     /locus_tag="PK264_00850"
     CDS             complement(24753..25931)
                     /locus_tag="PK264_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6361 family protein"
                     /protein_id="HRD74478.1"
                     /translation="MSSLAWIDFDEAERQRAQRIMALFQERESRDELGLGAIRDSIAD
                     HLFPGTSTIQTRLRYMLFIPWLFRTLEGRDVPESQLRTEARALEIRLADALKAGGESN
                     GIIGRDAGPRLQRLPSSVYWAGLGAWGIRLFPGSIDSLFISLRGLKWPRVSSDSEDAA
                     AGSRAAAIWNPALPQAPNGLLDCAAFSLTADEAQFIIDRLVATQPAALLTMLARDGID
                     AECDHIWTHPHLASFPADARRLVRHGEIFSHVMHGAALLYNLALSELRGRDDWVADYR
                     ERIAAWAAELDLSVVRNWSLDDFWDAVEHPAHSIRPAAKRFVAQWLELVVDGTTQIAS
                     APAARQLVEERERRLKTSQSRYANHAVRDRWTGASGVDRLNFRWNQARSHLRDLAHAE
                     "
     gene            complement(25950..29354)
                     /locus_tag="PK264_00855"
     CDS             complement(25950..29354)
                     /locus_tag="PK264_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRD74479.1"
                     /translation="MNAPLKPLADDVARRTVIAVHDRSFLVEAGAGSGKTAVMAGRIA
                     LMLAEGIAPRTIAAVTFTELAASELLIRVRDFVGELVAGRIPAELRVALPYGLSEDQR
                     RNLAATSVAIDEITCSTIHGFCQRLIKPYPVEADIDPGAVVMDRDQADLAFAEIVEIW
                     LREELADDAGGLLAEMVLQDPGETVWLIHKILTHLRSRRTLAVEAPEALEPLIGAVRQ
                     TADGFVSWLHTAGVDEPETADIATLFLEKAEAIEAISPAEPADLVRLLLARAHPDLCT
                     AGGSFRAYKKKGKWCEAAKHAGLAKADGDRLNAGAEGHYNACCEAWTALSQAAASRIL
                     AEFVQLVQPVMDRFRDYKRSAALLDFDDLIFAARALLRDHEAVRRALAARFAHVLVDE
                     FQDTDPLQTEIFWRLCGDPQAGADRDDWTTFQIRPGALFLVGDPKQAIYRFRGADVAA
                     YIQARETFLAQAPDSVVSISTNFRSCEPILNYVNDRFELLLSAENGQPGFTALDAFHP
                     ERNDGLCVAALDIAVADADGKASAERQRDGEAEAVADMCARLIGSEAILDRKTGERRA
                     CRPGDIALLAPTGSDLWRYEEALERRGIPVATQAGKGLFRRQEIQDLIALTRVLADRR
                     DTLALGALLRGPLVGLTEEELLDIVWALPRSEDAPDALPRLDLGVAPETITHPHARDI
                     IEKLQALRLRMNATTPHDLLSQAIDVLRVRPILLHRHRDQAERALANVDLYLSLSRAY
                     AVRGLRAFAEAMTAAWTDEARAVEGRPDAQEEAVALYTMHAAKGLEWPIVVPVNTMTQ
                     IMAPESAITDRATGRFYCPVLGVKPTGYEAARDAEKAELDRERIRLWYVAATRARELL
                     VLPRLDVAAKGSAWISLLDLSLTELPALDLSHLPPEVGSDGAGAQNAQTREIFAAEAE
                     MIAERRQRIVWLAPSRDENATGPALHAELPEILVTDVDDAPVEDSIVQTIQGGRERGV
                     ILHKLIEEVLTGETAETEPALIARAEALIRAIGQPVAPDPAQGLAAAELAACVLRALA
                     LPEIVALRPGLLPEFPVYASILTEEQEEATAGIADAIAFGPDGAPQVIVDWKSDVAPA
                     PETLDHYRTQVRAYLDMTQAERGLIVLVTSGAIIPVGRTNPAVAVA"
     gene            complement(29354..32014)
                     /locus_tag="PK264_00860"
     CDS             complement(29354..32014)
                     /locus_tag="PK264_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRD74480.1"
                     /translation="MQPKERRTIVAHGRLAMREVRLAAARERRHGLQIITFEGLAARL
                     AGGLSRPVDDETLRATIQRMLPDVELGELDGLKALPGMVGAATETLRKAWRAGVDLQA
                     RASEHPRIASLASLEEAVLAALPPAMMRPVDLVAAALQRLDHVDALFGPIEIVGITEL
                     SPCWRPLLHAIAQHVPITWSAGPRSTPAWLDGAVIDVTRSDPHTPEIVTVSASTAYHE
                     AIEALRWARQLVASGAADPAEIAIASVTPADYDDHFLALRADANLDLHFVHGVKITAC
                     REGQAAAALADILVRGLSQTRMRRLSSLLAAYSGAFQALPAGWTRVLPADAPLASPRA
                     WTHLLDRLTAADWPDEIDHGPALREIIGLLSQGVAAAEAVGEALLNGRTLAIWRKALL
                     AGPAASLDLTLETLKQDDGLDACVSVAWMPASALAASPRRFVRLLGLNSSRWPRGISE
                     DRLLSDHIIPTAELDPLPVGVADRRDFATILATSERQVVLSRARRDSDGRLLGRSTLL
                     QGQPDEVYLRRNRVPDHAFSETDRLTARPQEFRALPQAVSAAACWRNWLREEITPHDG
                     LVRADHPVIQAILARTQSASSLRKLLRNPLGFVWHYGLRWRAPESGDDPLVLDARAMG
                     DLVHQILDRALRTLEANGGLAAATGQQIASAVDGAAGEVAESWETERPVPPRVIWRRT
                     LDEARELSRRALAFGDERLPDARAFGEVPFGGAEPKTNDALPWDATASVGIPGTDFRI
                     AGYIDRLDVSADGRRALVRDYKTGRKPKDTIILDGGKELQRCLYAFAVKAMLGDDVAI
                     SASLLFPRDEIDLRLEDPDGTLADVAGYLSAAHASLLAGGAVLGADTGGTYDDLAIAL
                     PANAGATYCKRKIAAATERLGDAAQVWEAQ"
     gene            32188..33045
                     /locus_tag="PK264_00865"
     CDS             32188..33045
                     /locus_tag="PK264_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRD74481.1"
                     /translation="MDAAVALKWGVERRLEFIEFRLFWDGGVNRSDIIDVFDVSVPQA
                     SKDLTLYQERAPHNAVYDKSAKRYVASPQFSPIFLKPEAEQYLNQLRSVADGILDSSE
                     SWIAHLPPYGGPPVPARGVNTETLRTIVAAIRQKEAVEVKYQSLSSDDPRWRWIAPHA
                     IGFDGYRWHTRAFCEVDRTFKDFLLSRIIETRSLRPSEVSAEHDTDWNEFVTLEIGPH
                     PDLSESQKRIIALDYGMRSGRAQIRVRKALRYYALRRLGLDTPPAARRPQDQQIVLLN
                     ERGVDEDHR"
     gene            33029..36301
                     /locus_tag="PK264_00870"
     CDS             33029..36301
                     /locus_tag="PK264_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HRD74482.1"
                     /translation="MKIIDNTSQLFGDDLKATVGRGARLKIAASCFSIYAFEALKSEL
                     SKIESLQFIFTAPTFVPMEVTDRLRKERREFFIPKARRESGLYGTEFEIQLRNKLTQR
                     AVARECADWIRKKARFRSNTTKAPMQQFLHVASDDGGIAYMPIGGFTAVDLGYQKGDA
                     ISNFVTRFDDPNHTQMYLQLFDQIWSDPEKLDDVTAAICDHIESVYQENSPERIYFLM
                     LYNIFQDFLEDIDEDVLPNDLTGYRDSVVWNKLFNYQKDAATGIINKLETYNGCILAD
                     SVGLGKTFTALAVIKYYELRNRAVLVLCPKKLADNWRNYNTNLTTNIFAKDRFNYDVL
                     CHTDLSRTSGESFGIPLNRVNWGNYDLVVIDESHNFRNNDVFKDRETRYQKLMNKVIR
                     AGVKTKVLMLSATPVNNRFTDLRNQLALAYEGQSEALSQNLKAQTSIEEIFRRAQKAF
                     NAWTILPPDERTAASILKALDFDFFELLDAVTIARSRKHIETFYDTKDIGKFPQRCKP
                     LSFHCPITQRPDVMGLNDIFTQLSVLKLAVYAPISYILPSRLRKYEEIYDTQVEGGRG
                     KLRQADRERSLQALMTTNLLKRLESSVEAFRLTLRSLSGNISRALDAIRDFERSGGTQ
                     SVSDFLADEELLDAEDDDLAGLGDITVGKKVQISLADMDLPSWKHDLEADLALIEDLI
                     ASMDKVGPDDDAKLQHLMQVLGQKVSEPLNPGNRKVLIFTAFADTANYLYANLAPFAQ
                     QLGLQTGKVTGSGAPKTTLRKSYDFQGVLTLFSPRSKEKAVVLPNEPGELDILIGTDC
                     ISEGQNLQDCDYLVNYDIHWNPVRIIQRFGRIDRIGSPNTQIQLVNYWPDITLDEYIN
                     LKERVENRMVIADVSATGDDNVLTAKSSDIAYRKDQLRRLQEEVIELEDVKTGISITD
                     LGLNDFRMDLLNYVKEHGELDNLPNGMHAVVPAQPDLGLRPGAIFALKNIHDSVNINQ
                     QNRLHPYYLVYIGNDGEIVADHTEVKRLLDLIRTSCKGRPEPIHEVCRAFNERTQDGR
                     KMDRYSELLSSAIRSMIEVKEEKDIDSLFSGGRTTALVHTIAGLNDFELIAFVVIEGA
                     DA"
     gene            36298..36990
                     /locus_tag="PK264_00875"
     CDS             36298..36990
                     /locus_tag="PK264_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HRD74483.1"
                     /translation="MTAFFDYPKSAAFGRVVPKNKIYEHAGANTALKDLFVREVDQIL
                     WRFKLAPETTNLGATSAVTEIQVFGISLKTGRLDEAVLRAIDKAIPFPLIFELTWRGK
                     RKATAAFKRPSEADGSKWVISEYFATEWASEDAPREPLPVALDLGALYDRLLTAMMPA
                     SAQISEHIQTRVDRMEAIRAKTRELERIKARLAREKQFNKRVAINAELRAAKQELERL
                     TAGDPVGAVAEK"
     gene            37001..38941
                     /locus_tag="PK264_00880"
     CDS             37001..38941
                     /locus_tag="PK264_00880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD74484.1"
                     /translation="MEKLKMHSPVLSQENLANVRALFPNCVTEARDEATGKLRLAVDF
                     DQLKQELSDYVVEGPQERYRLDWPGKREALALANAAVAKTLRPSDNESVDFDGTKNLF
                     IEGDNLEALKLLQETYLGRVKMVYIDPPYNTGNDFIYSDDFSESAESFLARSEQRDEG
                     GNRLVANTTSNGRFHSDWLTMMYSRLKLARNLLSSDGVLFVSIDDNEAHNLIRLGCEI
                     FGEENFVAQLAVMVNPRGRHLDRFVAKTHESILVFVRDGLNAGAIQGLEKEGRMVEEY
                     NKSDDKGPYRLLGLRNRNQSFNPTTRPNLYYPLYVNPLTREVSIEQDDRFKVEVWPDA
                     PDGVKTCWTWGKEKVRNEGSLLCAEPYGDEWRIYRRDYLNGDDGETSRSLVKSLWTEK
                     EFTNDYGRKAIKDLFGSALMDFPKSPDLISRLVKIGTTRECIVLDFFAGSATTAASVL
                     SVNAEDGGNRAFIMVQMPEEVEQSSAAYKAGFHTIGELAKERIRRAGAKTLEGDCHPE
                     WNRDVGFRVLKIDTSNMQDVYYRPDEVDQKDLLEAVDNIKPDRTAEDLLFQVLVDWGV
                     DLSLPIRRESVQGKTVFFVDENALVACFELGVTEELVKELAGREPLRVVFRDNGFVSD
                     AVKINVEQVFRQLSPGTDVKSI"
     gene            38952..41990
                     /locus_tag="PK264_00885"
     CDS             38952..41990
                     /locus_tag="PK264_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD74485.1"
                     /translation="MKLKFKVQPYQTNAVESVVDCFAGQPLVSGVTYRIDPGRKAQAS
                     AFEEGFKNADIALTDLQVLANIKEVQRRQNLPMSDALVSSAGCRFNLDVEMETGTGKT
                     YCYIKTIFEMNKRYGWSKFIIMVPSIAIREGVYKSLQITADHFTESYGKKARFFIYNS
                     KQLHELESFSSDAGINVMVINIQAFNARGADNRRIYDELDNFQSRKPIDVIASNRPIL
                     ILDEPQKMEGAATMEALPKFKPLMILRYSATHRTQHNRVHRLDALDAYNQKLVKKIAV
                     RGIQTRGLAGTNAYLYLEGIDISKKSPVARIELEVKLKSGEIKRQLKRLEFRDDLFSE
                     SGELDQYRDGFTISQIDARNDTVEFTNGAVLRAGEATGDVSERDIRRIQIRETIKAHL
                     DKEKQLFAQGIKVLSLFFIDEVVKYRDYERPDEKGEYARVFEEEYELLKAEYLSELAI
                     DNEVYRKHLGAIETSKTHNGYFSIDKKTNRLKDPTVGARSVDSDDVDAYDLILKDKER
                     LLSFGEPTRFIFSHSALREGWDNPNVFVMCMLKHSDNTISRRQEVGRGLRLSVDQHGD
                     RMDHPAVVHDINVLTVVASESYKDFVGGLQKEIADTLSSRPRKATEAYFTGKTIATES
                     GPVEITPAMAKQIYRYLVKNDYSDDADQISKTYHEAKEAGTLADLPDELKPHAEQIFQ
                     LVDSIFSDAQLPKVDDGRKPKTNPLNDNFEKKEFRELWARINQKAVYRVEFDSDELIR
                     KCVNALDSQLRVTPLQYTVQTGLQGDGLTDDQLKAGDGFKLTGSTTERGGSVHSLVRY
                     DLLGNVAENTELTRKTVAEIFTGVAASVFGQFRENPEHFISEASRIIAEQKATMIIER
                     LAYDGLSDRYDVDIFTANQTGQDFSKASSKLKNHVYDYVVTDSDIERRFADQLDASSE
                     VVVYAKLPRGFLIPTPVGDYNPDWAISFKEGSVKHIYFVAETKGTMSSMKLREIENTK
                     IECARKFFDEIGRRVTEDRVKYDVVTSYEKLMDIVGRAAH"
     gene            complement(42577..43170)
                     /locus_tag="PK264_00890"
     CDS             complement(42577..43170)
                     /locus_tag="PK264_00890"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HRD74486.1"
                     /translation="MAPPSPGPLPPADFASRELPIEEIPSGARLIRIHRSDRGPLFFG
                     STGGNRFDDPAKIYGVCYLATTLEGAFAETCLRSVGARFVALTFLEARSFSEIEVSAP
                     LRLVSLHGPGLAQIGATGAVTSGPHAVAQQWSQAIHDHPTVPDGIAYRSNHDNGEICV
                     ALFEHVGDHLAPSDPQPMTTNRTRLAKLLARYKVGLG"
     gene            complement(43157..43648)
                     /locus_tag="PK264_00895"
     CDS             complement(43157..43648)
                     /locus_tag="PK264_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74487.1"
                     /translation="MAAVGASTDTDVLFSSLREAAAIGAEIEPDKPDPLTEAFLRGSE
                     MKREMLKAEGGALSAQQLAEHLGITAQGLGRKRERNQVFWLDVGDGYVYPAFQIGKNG
                     LLPGIREVLDAFTVDDPWMRVNFMLTGDQRLGGKRPIDQLRKGKIEDVVTAAAAYGEH
                     GAA"
     gene            44354..46030
                     /locus_tag="PK264_00900"
     CDS             44354..46030
                     /locus_tag="PK264_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRD74488.1"
                     /translation="MNARATPALVPPGRAALYLRVSTGRQAEHDLSIPDQRRQLHAYC
                     DGKGIAVVAEFVEPGASATDDRRPEFQRMMDAAAEKPAPFDCIIVHSFSRFFRDQFQL
                     EFYVRRLAKNGIRLISITQDLGDDPMSVMMRQIMALFDEYQSKENAKHTLRAMRENAR
                     QGYWNGSRPPFGYKIAVAEQRGARAKKKIEPDPSQTETVRLMFRLARIGSEDGPMGSR
                     QIADYLNAHGIRTQTGGRWGVGAVHQILTRETYIGRHRFNVSGKRKGERKSEDEIVEM
                     AVEPIISEEEFAEVQKVMRSRSPQLRAPRFVSAGTLLGGVCFCADCGGAMTLRTSGKG
                     DQYRYYTCCTAARQGKSGCRGRTIPMNELDEMVVGHLEERLLAPDRLEELLSGLLKRR
                     EEHTERLRGRVAELRKQAADAEAKLTRLYEAIENGLADLDDCNLKGRIVELKRMRDAA
                     RADADRAESRADDKGKRLTPELLRRFGIEARKKIRARDGGFRRSHVQALVQRIEVGTD
                     EIRIMGSKPRLLQTLVTSGGGYGVETATHGVRSFVPKWLPGPDSNQRPTG"
BASE COUNT         8747 a        14080 c        15054 g         8160 t
ORIGIN      
        1 tgaagctcgg atcgtcgagc ttgaggccca gatagtcgcg gccctcgttg gagcgcttgg
       61 accaggcggc tccgatctcg acccggccaa cgtagacgcg gtggctgggg gcgttctcgc
      121 cggtggcgcg cgtgtcggga acgatgcgga cgttcttggc ctggacgctg agggtgacga
      181 tttcgccact gaactcgttg ttgccggtct tcttgaaggt gccgatggtc gccatgtcag
      241 ttctcctaga tcactgtctc gagcccgcac cattgcggcc tcgatggcga tcggcaggcc
      301 ggaggcgatc gacggcgcac cccgcagggg ctcgacagca aaggaggaac tttcttgcct
      361 cgcgaggaat gggcgtgagc ccaggggaag aaagtttcga cgccgctgtt gcgccatagg
      421 cgatcgaggc ttcagccggc ctacggctag atcaggccat tgaagaggcc gcttgggtgc
      481 gctcgatgcg cgcagggatc acgcatggag aacttggcga cccgtcgcga cctccaggaa
      541 acggcaacga cgttcagagc atcggctttc cgccaaggac gggcaggcac gccgtatccc
      601 gccgatagga acaccacggc gcggcgcctc catcctgcgg ccttgaccga ccgtcgagtg
      661 gggggccttg gctgcgagcg cgtcgagggc acgaacttcc acgcctcgaa agccgtgggc
      721 ctgacaccgt ccgatgatcg acaagaatac cggcgggaac ggagcggctt gggactgcct
      781 tggtccgccc gcgcgtcaat gatgttcacg ccgtggcgat gtcctgtctg gacggtccgg
      841 gcgaggccat cgtcgttatc cgaacgaagg ccgtgatgcc gaccgcaacc gcgccgatca
      901 cggagaagac gagctgccag gtttcggacg gcatggtgtg cttcacgatg ccgtgcgttg
      961 cgtggtagcc ggcgaacgcg gccggggcga cgaaggcgag cgcgaccaga agcttcaacc
     1021 acatcggacg aacgaaggcg agcaggagct ggccgaagcc gagaatcgcg ccggcggcaa
     1081 cgacgccgat ggcgacggct ccgagggcac cggcgccggt ctgatgcgcc cagacgccga
     1141 tggtcacgcc ggcaaaggca ggcagcgcaa acacagcgag cttgaagagc agccagcaga
     1201 gcacgcctat ggcggcgagc gatccaagga tggcgaggat gatcatggtg gtggtctccg
     1261 tgagaatgag tctgacggtt gcgccttcca ccaccaccgc ggcgcgattg gcagcatagc
     1321 cgaagtcggc tcgcttcggg agcggaaatc gaaccgacct ccgcccgcga aagcggttcg
     1381 cctccggtca ggaggcgaag ggatcgatct cggccacgat ctcggcatcg ccgtcgaact
     1441 cgttggaggg gacgacggag agcgtgccgt ctccggccct gaagatgacg atcgcgacca
     1501 tcagcgtggt ggcgaggctg aaggcgaagg cttgagcgtc ggtgagagac attcgttccg
     1561 gctcctgtct ggaggcgggg gaccatcccc cgcgcgacag gcgcccgatg tgtccggccg
     1621 aggccgcaat caccgcggag gcggagcctc aaggccgcac gctcgcgtag cggacccttc
     1681 acgggttgat ggcgtcaggc catcggctgg accacggatc agcgcatagg aagcggggtg
     1741 gtctgtcgcg gaagcggagg ccggaacatc caaaactaag cgcgattggg gcgagccgtc
     1801 acggcgacca ccacttctgc cggccagcag ctttcaatga agggtgtttg acctgtcaga
     1861 aagaagcgat ccagttcaac atcgccacgt gattcaggtc gatattcaga gattctactc
     1921 gtaattttac agtgcctgag tgaagaatcg ccggcaggtg tctgctgcag cttgactggg
     1981 cagtcccgag gacgagggtg cgataaccca agggggggga tctgaatgtc ggaagtgctg
     2041 ggatgcaagt cgttcgcctg aaaatctccg gcttccgagg cgtgcgttcg gcggacatcg
     2101 tattgggccg ccatgccgta ctggtggggc caaataatag cggcaagacc accgtgatcg
     2161 aggcgttggc gttgctgttc gggcgcgatc gtctggtgcg ccgcctgacc gagcatgact
     2221 ttcatggcag cgcgcccgac gaaaccgccc gcatactgtg catcgccacc gtcacgggct
     2281 ttacgcccaa cgacccgcac caccatttgt cttggttcaa tcccgaacgc ggcgtcgaga
     2341 aatggttcga cccgaaagcc aagacattga gcgccgcgcc ggacgcgcgg cataccgatc
     2401 tggcggtgca gatcggtttc gctgcgcgct tcgacctgga cgaactggaa gccgaaacgc
     2461 tccgtttctt cgtggatgac gaggccacgc tgggcgatcc gttcgcggaa gacgcgcatc
     2521 tgcgcacgat ccacaccaag gtgctccagg agttgggctt ctttctggtc ccggcctcgc
     2581 gcacatggga ccgctggatt tccttttcct cggagttgtt ccgccgcgtc gtggcgacgc
     2641 gtggggacat gcccgcgcag gcagtgcgcg ccgagcgcca gagactctgg accccgccgg
     2701 acggtgcgcg gctcgaagac cagccggggc tttccgagat cgtcggcgcg gccaatgacg
     2761 agttgcgcgc gctcatggcc agcgccccca gactccagtt gcgcctgacc gcgactgaca
     2821 gcgactcagt tttggaaagc gtggtgccgc acttcgtgca aggcacgggg ccaacccttc
     2881 cctcgcagcg gcagggcacg ggccttgtct cccttcaaag ccttctattg ctcatgcagt
     2941 tcggcaaggc gcgcgcggaa acgggccagt ccttcatcct ggccgtcgag gagccggaat
     3001 tgcacatcca gccctcgcag caaaagcggt tggtcaaccg cctcaatgca ctgtgcaacc
     3061 agacgatagt cacgacgcat tcgccgatcg tggccgcaat gtttccggct cctgatacgc
     3121 tctttatcga aacgcgcgac ggagcgttga gcgccaagcc actgatggac gctgtgcccg
     3181 cgcagcctac taaccaccag cagcacttgc tctacgcatg gcggcagaag ctggtggccg
     3241 cgctcatgca cgaatgcgtc ctgatccccg aaggggtttc ggacgtggca tggcttgagg
     3301 cgctgcaaac cgctcttgag cagcatcagg gttgggataa cgcgcaagcg aatacgccgc
     3361 ttctctccac tttcgttggc atcgtgccga ccatcgacgc caagattgcc gacaccttcg
     3421 cgctggtcgg cgccgttcac gcgcggccct gcatccttgt ggacggggac aatgacgggc
     3481 gcggctactt cgatgcggtg aagggcagca atccgccgcc gcgctgcgtc gtcttctggc
     3541 cgcagggctg ggcgatggag catgtggttt cgtggattgc gggcgcggat gaagccgcga
     3601 tgctgaccgc actcggaacc gcgctgggtg ctcctttcgc cgaccgtgag gcgctcaccg
     3661 cacatttgct tacccagaaa tcctacgcgc cgacccatga aagcgccgtc gtcacgctca
     3721 tggcgaatgc ggcctgccgg gcgcgcgccg cgcagttgct caatgccctg tgcgacgtgc
     3781 tgcgcgatcc cgccaacgcc aatacgccgc tctttgcgcg catcccggcg gattctaccg
     3841 ccgacatgca cgtcttccgg ttcgtgccca catgacgttc gattttttcg agggcgcagc
     3901 gggcacgggc aagacccaca atctcgtggc tcgcgcggga gagatcgttg gggacggcgc
     3961 gctgggcgaa gggcacaagc tcttggcgct caccttcatg aacggtgcgc gccgacgact
     4021 ggatgcgcgc cttggtgaga acccggcgtt ccgcaggcgt tttgactgcc aaaccttcga
     4081 tgtgtttgcg cggacactgg ccgcgcggcg cagaagcctg attacgccag cgatgcaaga
     4141 gcaggccatt gcactgaacg aatttgacgg gccatgcgct ctggcggccg ggcttctgga
     4201 tcgcgagcct gtccggcaat gggtggcgag aaccttcccg ctggtccttg ttgatgaagc
     4261 gcaggatctc gacgaacacc gcatgggcat ccttcagggg ctttcacagt cctgctgggt
     4321 cgtcgcgggg gcggacgcct ttcagtgtct tcacgacggg cgtgataccg cgcacctgat
     4381 ggcgtggctt gagggcgcag gacacaccca ccacctcact cagcctcggc gtacaaatca
     4441 acagggtctt cttgccgccg cgctggctgt ccgcgaagga cgcgacatca aggccgttct
     4501 tgcggccaac acattcaaca acaggacaac atggaacggc gcgggtttcc gtcttctgga
     4561 aacgtctgcc acccagaata acactgggct tctggcatgg gcgattgcca atgacttttc
     4621 gcagcgccaa ggcgcggttg ccgttctgac gcccgatgcg ggcaatgcca tcattcgcgc
     4681 agctctcgcc acgattcaga cgaggcagtg gacacgtaat aacggcgcga ctttcgggcc
     4741 atatccgcac acatgggatc gtcacgacaa cgaagaggcc gacgccttgc tgggcaatat
     4801 cgccttgccg gaggcagcgt cttgcgccga tcttcgtgca cttctaacgc ccttggctgg
     4861 acacgccgcc atcgcgcagg ccatcagccg catggaccgc ctgcgtaggg cgcacgggaa
     4921 agcggccttc accgccgcgc aagtcaccga gttcgtccgg gattccgtgc gcaatcgttc
     4981 gcgtctgggc tttcggcagc aacatggcca tctcgccatg acgatccagc gcgccaagaa
     5041 ccgcgagttt ccgaacgtca tcgtactatg gccgcacact gctacaggca gcgcggagca
     5101 tttgcgaagg ttgctctaca acgccatcac gcgcgcgcgg aaccattgca ccgtcatcgt
     5161 ccttggccag ggacggctga acgctccgcc cttcgcataa ttcaccgtca ggtaggttgt
     5221 tgaggtgtcg gcctttatgg acgagctggc cggtgcattg catcaaagag gcggcgctca
     5281 cgcgccgccg aacctccaga ccgggatacc gagctttttg gctttatcgg cgaagttgtc
     5341 ctggatgccg gtgccgggga agaccatcac gccgatcggc agcacttcga gcatctgatc
     5401 gttgcgctta aagggcgcgg cgcgaccgtg cttcgtccag tcgggcgcga aaccgatctg
     5461 cggcactccg cggtggtcag cccagcggga ggcgatcttc tcggcgccct tcggcgattt
     5521 gccgtgcatc agcaccatgt cgggatgttt ggcgtggact tggtcgagtt ttgcccagat
     5581 cagtcggtga tcgttgaatt cgagcccgcc ggtgaaggcg atcttcggac ccgggggcag
     5641 gagcacttcc tgctcggcgc gtttcctggc ggcgaggaag tctcggctgt cgatcatcgc
     5701 cgaggtgaga ttgcggtggt tgaccttcga cccgttgcgc gggagccagg gcttgccggc
     5761 gtgacgctcg tagtgctcgc cggcctggtc gcgcatcaac tcgaacgcat cctgccgctc
     5821 gaccagcgtc ctgccttccg cgatgagccg ctcgagttcg accgatttga cttcggtgcc
     5881 atcctgctcc cgctgggacc ggcgctgcgc ctgctcattg tcgtcgaggt cgcgcgcgat
     5941 ccgctcggcg gcgcggtgga agacgttgac ggtcgaccag aggagatcgt ccaggtcggg
     6001 ttcgaggcgg gtgtcgccaa gggtggcgat cagggcgtcg aagatgtcgg cgatggcgcc
     6061 ggcgacggcg ttgccttcgg gcagtggccg cggatcgggt tcgtcctcgt aggggcggta
     6121 gccatagagc tgaagctcgg tcagggcgtg gtcggtcggg gaagaggtgt gctgcggttc
     6181 atggtcgtca tgctcgctca tgggatgctc cgtcggattt ggaccgcgac cgtcgcggcc
     6241 ttcatggcga cgaagccgtc gggcgggccg gactggcacc cggagcggca gcgcagggcc
     6301 gaagcgcagc ggagggccga aggggccgac tattttggtt cgcgatggaa aggcccgaag
     6361 ggccgccgga aaatagtcgg ccccaaggtt gcctggccgg accgtttgac ggccgatcgc
     6421 cctctcgaag gccgaggcgc gggcctcttg cgatggggag catccggcga cgcggtcgac
     6481 gacgagccgc cccgcatccc attccggcta cgctgccagc tccatgaagc gggcgacgtc
     6541 ctgcggcgcg atctggacgc ggattcctgc ccgcagagca tcgatgccga gcatccgcag
     6601 atcctcgttg aagtccccga gcgccggcga cagcacgatc gcctcgatcc cggcctgttc
     6661 cgcccggtcg atcaatgccg ccatcgcgcc atctcctgcc ggatcgtcgt cgcgcgcgat
     6721 gtagagccgg cgcagcacgt ccggaaagag gatggctgag agatgggcgg ccgagagcgc
     6781 ggccaccatc ggcatggtcg gcagggcgca tctgagcgac agcatggtct cgatgccttc
     6841 acctgccgcc atcaccgagc ccgccgcgcc gagtcgcacg gcgtgaccga gaagatcgcc
     6901 catcgcccgt ctcggcgtgt cgatcggggc cttgccgagt gtcgcttcgc tgaagccgtc
     6961 ggggtcgagc caggtgcgat gtgcgccggt gagcttgccg cccaagtcgg tgacggcggc
     7021 gatcatcgcc ggccacgtct cggtcggcga atgctcgtcg ggccggtaga agcagcgcgg
     7081 atggaaacgc agcgagccgg tttcgtgcaa agccgtaatg ccgcgtttgc gtaaatacgt
     7141 ctctgcgaga gtgcgtgtca tcggctgcga tatggcaaag agccgccgtg cggcctccgg
     7201 cgatccggtc ggtgcgttcg gcttgcgcgt ccggcgctcg tcggattccg gttcgggatg
     7261 cggtaggctg agaaagcgcc gagcctcgtc agcgacatcc ttgaagtcga ggaggctgca
     7321 cgcctcgcgg atcacgtcga gcaagtcgcc atgctcgccg gtcgccgcgt cggtccattt
     7381 gccggccgcg cccttgccgg tctccggtcc cttgagacgt acgaacatcg aacggcccgg
     7441 cgtgttgcgc acgtcgccga ccagccagta gcggccctga cggaagccgg aggagagata
     7501 atgccggcac accgcctcgg cctgccggcc gagacgctgc gccaggtcgg aagcgtcgtg
     7561 acggaccatc acgcagcctc ccgttcggag acgcgctcga cgggatagcg ttccagcact
     7621 ttggccagca cgccggcacc gttcgcgtcg ctcggcacga acatgcgcaa cttccacgaa
     7681 atgatctcgt gaaacaggcc ataggcgcgc aggcggtcgc gcatggtgtc ggtgaagccc
     7741 gatagttcga tgcgatgggc gcccatgacg cgcacgcggc gaagctggag cccttccgca
     7801 aggtcgagga tggtgttgcc gtccagcaat gccgcgaagg catcgcccgc cgtcagcgtg
     7861 ctggggccgg tcgcgagcgc gcccacgacc caggcgggag agacgcggcg gccgatgatg
     7921 cgttcgccct cgtcggtctg gagccgatag acccgcgtcg actcgttcgg cagccgcttc
     7981 cagatcggca gcaggaggcc ggcgacgatg tggatcgtcg agtcggagaa ctccggcacc
     8041 tgcgccactt cgcgattcca ggcggcggcg aaggcgtcgc gatcggcttc ctgccagtgg
     8101 ctctccgcca tcaccttcag ggaagcgtga tgatgctcca tgggccgaag caatcgcacc
     8161 cggcgttcga tctcgccgtc gtcgagcatc acgctgggcg ctgggatctg caccgcaggg
     8221 cgccccgagc gctcgttgac gagcaggacc gcgcgaggat cggagaggtg gtcgagcgcc
     8281 tggccgaggg cgagcggccg gttgcgctcg cgctgcgcga tggtgaggag gcgcgtttcg
     8341 gcaccggttg cgggatgggt gtagatggtg cgccggtcgg cgacgacgaa gctctccgct
     8401 ttcagcgtct ccagtccgat atcataggtc ccgctggtaa tagcgccctc gatcctcgca
     8461 ttgaggagct gctcgaaggc cgtgaacagc acgccctgca ggtcgatggt gagcgccagc
     8521 agccggttga ggaaggtggt gatcggcggc agctcgtcct tcaggccgcc atcgtccatc
     8581 agggagaggc ccgtagcgct ctcgaatgtc tggagcgagc aaccctcgac cttgccgcgc
     8641 gcgatcagca tatagagttg ccgcagcgcg acgcgggcgt aagggctttc cagattgtcc
     8701 tctggccgaa acaggccctg accgccggtc tggcgctggc cgcgcgtgat cgcgcccagc
     8761 gtgtcgagcc ggcgggcgat ggtggagaga aagcgctttt cggccttcac atcggtcgcg
     8821 atcggacgaa acagcggcgg ctgcgcctgg ttcgtccgat tggtgcggcc gagaccctgg
     8881 atagcggcgt cggccttcca accgggctcc agcagatagt ggacgcgcag gcgctggttc
     8941 ttcgccgaca gctccgcgtg atagctgcgc ccggtgccgc cggcatcgct gaacaccagc
     9001 acgcgcttct ggtcgtccat gaaggcggcg gtctcggcga ggttggccga gccggcgcgg
     9061 ttctcgacca tcaaacggtc gcccttgcgg acaatgcggc gcgagcgtcc ggtcacttcg
     9121 gccactatgt cggtgccgaa gcgctggacg atctggtcaa gcgcgccggg aacaggtggc
     9181 aagcttgcga gctgctcgat cagccggtca cgccgagcga ccgcctcgcg gctctcgacc
     9241 ggctggccgt cgcgatagac cggccgggag gagagattgc cttcagaatc ggtgaagggc
     9301 tcgtagagct gtaccgggaa ggaatgctga aggtatgaca ggacgtattc gcggggcgtg
     9361 atgtcaacgc gcacgtcatt ccattcctcg gtcggaatct cggccagtct tcgttccatc
     9421 agcgcttcgc cggtcgagac gatctgcaca acacatgcgt ggccgtcctc aatgtcgcgc
     9481 tcgattgagc ggatgagcgt cggcgtcttc attgaggtga gcagatgtcc gaagaagcgc
     9541 tgcttggtgc tctcgaaggc cgagcgcgcc gccgacttcg cttgacggtt cagcgtgccg
     9601 gtctcgccgg tgatgttcgc cgcctgcatc gcggcgtcga gattgttgtg aatgatcgcg
     9661 aaagcgccag cgtaggcgtc atagatgcgc cgctgctcgt cggtgagctg gtgctcgacc
     9721 agctcatatt cgacgccgtc ataggagagc gagcgcgccg tgtagaggcc gagtgatcgg
     9781 agatcgcggg cgagcacctc catcgccgcg acgccgccgg cctcgatcgc ctccacgaac
     9841 tccgcccgtg tggcaaacgg aaaatcttcg ccgccccaca ggccgagcct ctgggcatag
     9901 gcgagattgt gaacggtggt ggcgccggtc gcagagacat agacgatacg cgcattcggc
     9961 agggcgtgct ggagccggag ccccgcgcga ccctgttgcg atggggcgac gtcgccccgt
    10021 tctcccttgc caccggcggc gttctgcatg gcgtggcttt cgtcgaaaat gatcactccg
    10081 tcgaagtcag agcccaacca ttcgacgatc tgcttgacgc gcgaaacctt ctcgccgcgc
    10141 tcgtcggacc gtagcgtggc gtaggtggtg aataggacgc cttcctgaag cgtgacgggc
    10201 ttgccctgcg ggaaccgcga tagcggcgtc accagcaggc gctccatgcc gagcgcggac
    10261 cagtcgcgtt gcgcgtcctc gatcagcttg tcggacttgg agatccagag cgccttgcgt
    10321 tgcccgcgca gccagttgtc gaggatgatc gcggccgatt ggcggccctt gcccgcgccg
    10381 gtgccgtcgc ccagcatgaa gccgcgccgg aaacggaccg cgcccttcgc atcgtcgggt
    10441 gctgccgtga cgagatcgaa ggtgtcatcg accgtccagc aacctgccag atagtcggaa
    10501 tgcgcctcgc cagcgtagat cacggtttcg agctgggcat cggacaggat gccgtcggtg
    10561 acgaggtttt cgggcaggcg cggacgatag ctgggcttgg gcggcgcgac cgaggccatc
    10621 gccgccgact gcacgagctt ggtgggatgc gcctgagagc cgggaatacg gatcgcctga
    10681 agtccgtatt cttcgtagat cgcatcggtc aggcgtgtgc tctcggccgg tgcccaatcc
    10741 acggtctcat agctgaggtc tgcgccctcg ggttcggcga gcgaggcgtt cgcggccggc
    10801 gtcgaggcgg cccgtgcgag atagccgcgc acggtcttcg gtacaggacc agatgcaacg
    10861 ggagcggcga tggtcgcggc tacggcagga cgaggcggca catgctcggc gatccagccg
    10921 atgagcgtta cgacatcggg ggcgatgccc ggcgatgcgg ggaatgcggc cgggtcttcg
    10981 gccggcgcct tgtcgatgac ggtgagccgc gtctcgatcg tcgtgccatg cttggcatag
    11041 accgcgccgt cgatcgcagc ggtgaacacc acgcggccgc gctgctgcag acggacaaag
    11101 gcgtcgcgcc aggccggcgc atccggcgag aaattggcgc cggtgatggc gaccaggcgc
    11161 ccgtcatcgg caaggcgcgc cagtgccgag gtgacatggc gcaaggcggc gtcgaccatt
    11221 cggccggaga cgttcgccat caccgagaag ggcggattca tcaacacgac gctcggggtc
    11281 accgacggat cgagatgatc gtcgatctgc gccgcgtcga agcgcgtgac gggcgcagcc
    11341 ggaaagaggg acgagatgag gccggcgcgg gtgtcggcca gctcgttgag ggtcaatgtg
    11401 ccaccggcaa tctcggccag gatggcgagc aggccggtgc cagccgaggg ctccaggacg
    11461 cggtcggccg gcgtgacggc ggcggcggtc aatgcggcta gaccgagcgg gagcggtgtc
    11521 gagaattgct ggaaggcctc gctctcggcg gagcggcgcg tgtgggtggg aagaagaccc
    11581 gcgatcttgg cgagcagcgg cagcgcggcg gccggagagc cggctttgcg gagaagcgcc
    11641 tttccgtatt tgcgcaggaa caggacggtc gccgcctcgc aggcgtcata agccgtcttc
    11701 cagtcccaga cgcccgacgt gtcggaagcg ccgaaagcgg tttccatggc cgagcggagg
    11761 attgcggcgt cgatgcgctg gccgcgctcg agctgcggga ggagttggcg ggccgccgtg
    11821 agggtggcgg cggcggtgtc gataggaggg acggacgaca gcggcgcggc cggggcggtc
    11881 gcgcgcgagg aaagactgtg catggggaaa gcctcgagga gagcgggaat gggacgagcc
    11941 tgccggcgct ctctctcgac cgcgcgggct caaacccgtc ccggcctgtc tctccctctc
    12001 aagccggtcg gccgtggccg acatgaaaca agcgcccggc cgtgaagcgg ggcgcttgtc
    12061 tgcgtaagga gagggaagcg ggacggccga agtcgccccg catcgcccga ctattctgcg
    12121 gcgaccggta ggtcgacctc tacggtggag ccgtcgtcgt cgcccgagag gaactccggc
    12181 agcgtttcga tgtcgccgtc ggtctctgca tcgctgcctc cggccaacgg ctcctcgcca
    12241 tccgcgaggc gcagcggttc gggcagccag ccggtttcgg ccagcagccg ttccgcctcc
    12301 ttggccatgt cgcccttctt gaggtgatcg atgagttgcg cggcccgttc gcccgcgccc
    12361 tcgcgcaccg cttcgagaat gcggcgcttc ggcacgcgac cgagatagtt gtcgaccgtt
    12421 ggcgcccagc cgacttccac catgtcgagg ccggtggcgt gggcgagccg gtcggcctga
    12481 ccgagacggc gttccaggcc gtgctgggag acgccgttgc cgccatacgg gttcggcttc
    12541 tcatagaggg cgttgacgcc gtagctgacg cagtgggcga gcagtgacag ccggctcgtt
    12601 tcgtcgagcg ccgcgagcca gacccacagc gcctggtcgt cctggggcag atcggccttc
    12661 cacgcctcgt gccgctctgc gattgatcgg gcggacgggc tgtccttgag atcggcggcc
    12721 tggaccggga agaagacgtg gcgcaccgag gcctccaggc atccgccgct cgtggcggtg
    12781 cgctggaagg tgtcgacgac gagcttgtgc agcagggccg tcaacgcgac atgcggattg
    12841 ttggcgaccg catcgcgcag cgccagcgtg cgatgggcgg tcagttccat caccagccgg
    12901 tcgggcagcg gcctgacggc atcgtcttcc tcgtcctcgg gctcggccgg ctggccgccg
    12961 atcgtgatga ccgcgcgctg cacggtggat gcgcccgctt cggcgccatc ctgatcggcg
    13021 gaggcgacgt cgccatcgat ctcgtgccca ggatcgacgg ccggcgcttc gtcttcgggg
    13081 cggacatagc cgcgatcgac aaagagcctg ccatcggggc cgatgctgac gaagacaccg
    13141 gcgcgagcga tgtcggcggg atcgtaggtg acggggcgat cgtcgaactg gccaagcgca
    13201 gtctcgatct cgccgagccg tgcgtcgacc tcgtccggca gctcgtcggc gtcctcatac
    13261 tcggcctcga gcttctggta ttcggcgttg agcgcgtcga tcgtcgcctg ttcctcggcc
    13321 gtcaggtcgg gcgtcacgcc gtcgagctca cgcagcccaa gagtatggcc gtaggggaag
    13381 tcggtcgcga cctcgatcca cttccagcct tcggcggcga tggtctccgc ctcggccttg
    13441 agcttcgccg cgaccaggtt gtcgagcagg gcgatgtcct cgagccagcc gccatcatcc
    13501 tgctggaaga gatcgcgcag gaccatgccg ccggccgctt cataggcgtc aaggccgacg
    13561 aacacggcgc ggcggtcgga ggcgcgcgcc gtcttctcgg tcagcatgcg gcggatctga
    13621 tagggctcct tcgaccagga gccggagatc gtctcccaga cctgctcctg gcgagcatga
    13681 tcggggctga cggtgaaggc catcaactgc tcaagagaaa tgccatcctc ggcatagata
    13741 tcgagcagtt ttggcgagac gctggcgagc cgcatgcgtt gcctcaccac attcgccgag
    13801 gtgaagaagg ctgctgcaat gtcctcctcg gaccgccctt tctcgcgcat cgcctggaac
    13861 gcgcggaact ggtcgagggg atggagcggc gccctctgga cgttctcggc aagcgaatcg
    13921 tcctcggcga ggatgtcggt cgccgcatcg cgcaccacac agggcacggg cgcgttcttg
    13981 gccaggcgct tctgcttgac cagcagttcg agcgcccggt agcggcggcc cccggcggga
    14041 atctcgaaca tgccggtctc ggccccctcg gcgtcgagga ctggccggac gttgaggctc
    14101 tggaggagcg tgcgccgggc gatgtcctcc gccaattcct cgatcgagac gccggccttc
    14161 acgcgccgga cgttggactg ggagagcacc agcttgttga agggaatgtc gcgcgaagac
    14221 gacagggtga tcttctgaac ggcagtagcc atcgggattt tctccgcgac gggcggccga
    14281 aagcctctct cccggcctcc aacccgtcac gaaaatcccc tcggccctct tcctctcgca
    14341 gcggcgaaag cgaaggccga gggctgaagg aggatggaac ggcggccggc gcgttataga
    14401 ctcctggcat ccggtcagcc cttggaacgg gggcgcgccg ccacggcggc cagaacgtcg
    14461 tccagcagtc cttcggccgc ccgcagcgcc cgttcggacg acaatggcgt cggctcgcgc
    14521 ctcgccagct cgcgggtgcg atggcagcaa taatagggcg acaagatccg cgtcagcatc
    14581 cgcacgtccg gatgacgccg atgaaatccg agccgttccg cggcggcgag tgccttgacc
    14641 aaatcgtgcc gaacctcagc gcggcaccga tcgtcatccc atcccgcgaa atgcagatag
    14701 gatttcaacg ccagctcgac agcgcatccg atcagttgga ggaagatctc gcgagaatgg
    14761 ggatcactcg acacgagatg gcgtgctccg gcaaggaaac caaccgcatt gtcgaagagg
    14821 tacttccccc gccagcgtgt gggggagccg cgcgccggcg cggacgcatg gctggccgcc
    14881 ggggacgacg cagcctcctg ccggtgacgt cgccggcgcg atgaagatgg cgcgcgcatc
    14941 aggccgcctc ccgctcgcgg gtgtcgacgg acaccgcgaa ttggtcatgt tcgggcagga
    15001 agctgaggag gtaatcggca gccttgctcg cctgcgatgc tgcgcggacg atggctctat
    15061 tgtcctcgcg caggacctca gcccatgagc cgatgtaatc ggcgtgccgc acggtcggca
    15121 cgatgccgag tgcagcacag caaaatgccg ctgaaatctc ggcgaccagc tcctcgaagg
    15181 cgtatttctt cgtgccaaaa gaaccggaaa ggtcgcgatt caggcgatgc ggtgccccgc
    15241 tggcatggga gcattcgtgc agggccgttc gatgccagtt gataggctcg aagaaagcct
    15301 ggggcggcgg gaccatcaca taatcgcgcg ccggcacgta gaacgcacgg tcgccaccaa
    15361 tccggaaatc gatcccgctc gccttgatca gcgcatccac attcggctcg atcaggccgg
    15421 gtccgggcgg cggcgcgaca atggcgaggt cgtcgggcag gccctcgcat tgcgccaggt
    15481 tgaaaaccgt gaagcgcttc aggaacggga tcgcctgggc ttcctcccca gtttcgcggg
    15541 cacgctgctt ctcgtcgtcg gggacgaagc gatcggcata gacgaccgtg gttccacgtt
    15601 cgccttggcg cacatttccc ccgagagaga gggcctgacg gaaagtcagc cagctctgac
    15661 ccgggaagcc acgctcgatc acggcacccc agaggatcag cacattgatg ccggaataac
    15721 ctcggcccgt ggcggcgttc ttcggcatgg caagcggcgc cttcgccgcc gccgttcccc
    15781 acggctggac ccaaggtagc cttccggctt ccagctcggc gaggatcttt ccggtgactt
    15841 cgtcgtagag gctcgtccgg tcggagccgg aacgggcgcg cgtagcatgt ctggacatcg
    15901 cggatctccg cgacgggcgc cgggagcctc tctcccgacc ctcaacccgt cacggcaatc
    15961 ccgtccgcac tctcactctc acccggtctg cggtaagaag cgcagaatcg catctacggg
    16021 aatacggctt tccggctctt tgcgtcaggg atggaagccc ggaagggaga agacccgctt
    16081 ggcggggctt cagctccgcc gacagcccga cccgaaggga gacgtcccaa ccctgcaggc
    16141 catgccagat tggttgagcg tcatctaagg ttcttgtttt tccccctcat ttgaggcaaa
    16201 aaaaccggaa cctgttcttc tggaggattc gatgcctgcg acctcgccca tttattctga
    16261 acccgacgag gtgagcaccc ggttggcctc catgggtctg accgaagagc ctctgctgca
    16321 tgcggcacgg cgttggcatt tgtcctggat cagtttcacc gccaatcacc cgccattcgg
    16381 catcgggatt gccgcctgga tggaggcggt ggcggctttg cgtgaagggc tattgcccat
    16441 tggctggacc cgctccgatg agaagaacta tgctcttgtc ctccatccga accggtcgat
    16501 ggcgatcaat gtcgcgacgg gcgacgctgg gactgggctg ccgactgcga atgtgagcaa
    16561 caaggcgccg aaaggcgtca gcaccgccga tgcgatctcg gtcaatcagg tgcagctcga
    16621 attggatttg ccggtgccgg atatgcccca tatgcgtggt gatgaggggc cgctgacttg
    16681 gttcctgctt cttcaccgcg ccgggaccga agtccgttgt gagctgtcgt tgccctccaa
    16741 gatttcgccc gacggtcgca tcacccgctg gcaggagcgg atcatgttgc catccgttcc
    16801 gctggacgat tctgagaccg aaatcgtggc accgttgggt ccggatctcg atatcgacgt
    16861 gaagcggaaa gcatgagtgg cattgacgtc ttcaacccga ctcgcctgac ctgggcacgc
    16921 cgccggcgcg gacttaccaa gacgcgtctg gcagcgagca tcggtgtcga gttgcgctcg
    16981 gtgtcggctt acgaaaccgg tgagttcaac ccggagcccg atcgtctgag cgtcatcgcg
    17041 cgcaccctga agtttccgca ggagttcttt ttcggcgacg atatcgaaga gccagcgatc
    17101 gacacggcca gcttccgatc cttgtccaag atgaccgcgc gccagcggga taccgccctt
    17161 ggcagcggcg cggtcgccat gctgctcaac gaatggatcg aggcacgctt tgacctgccg
    17221 acccccgatc ttcctgatct tggtcgggac gctaatcccg agagcgcggc tgctgccgtg
    17281 cgtcaggcat ggggattggg cgaactcccg atcaagaaca tggtgcattt gttggagtcg
    17341 aaaggcgtgc gcgtctattc gctcgccatc gacgcggttg aggtcgacgc cttttcgatg
    17401 tggcgacgcg atcgtccgtt cgtctttctc aacatgctta aatccgccga acatgggcgg
    17461 ttcgatgcgg cgcacgagtt gggccacctg gttctgcacc gacatgctgc cccgagcggt
    17521 caggaggcgg agcaggatgc gaacgccttc gcctcggcgt tgctgatgcc gccggcgagc
    17581 gtgcgggcgc acgcaccgag attcgcgacc atcgacaatc tgatgcgatt gaagaaggct
    17641 tggggtgtct cactcgccgc gatgacgtac cgtctccaca agctcgatct cctttcggaa
    17701 tggcattacc gcaagctcta tatcgagatc tcgtcacgtg gatatcgcaa gaaagaacct
    17761 gatgggggac cgcgcgaagt ctcacaggtg ctgcaaaagg tgttctcggc gctgcggacg
    17821 gagggggtca gcaaggagga gatcgctgag gcgctccggg ttcatccgac tgatatcgac
    17881 gagttggtgt tcggcctggc gttgacaagt ctcgatggca ttgcacgccg aagcgaaggg
    17941 gggcggtcac ggccgtcgct aaccctcgtt tcgtcaaagg actgagcgat gctctcgctg
    18001 gcggaggcgc aagctgtcga aaatatagca gatctgctct acgagttttt gcccggtagt
    18061 ggcaacaaca agacggcctt tcccatcgcc gccacccaag ccggcgtcgg ggaattctgg
    18121 atgggcggaa gcaaacggcc ggccattgtt gaacttctga agttgaccct tgatcgccgc
    18181 cgggaccgtt tcacggcatt gattatcgcg atcgtccacc agtcgatgac gtggcggcgc
    18241 ggtaagggca atcctctgac ccgcgacgag attgatcgtt tgaacgcggc ccttccgggt
    18301 gtttcgttca agatcccaga tctgctcgat ccggctttcc tgaagagctt tggggagcca
    18361 gcgaaggcgg ctcccgcatc ttccagggtt atcttgtcgg aggcatcggc gcaggcactc
    18421 gttgctcggc tgatggaggt gagcggattg ggtccgcagg aacgcgggtt gcgctttgag
    18481 ggattcctta cggatgtgtt cgctgcgttc gacctcgcgc cgcgcggctc ctttcgcatc
    18541 gtgggcgaac agatcgacgg gagctttcgg ctccacaccg aaacatatct ggtcgaagcc
    18601 aagtggcatg ggccgcagat cggctttgct gatctcatgg cgttttccgg caaggttgcc
    18661 ggcaaggctg catgggcgcg cggccttttt gtcagcaatt caggcttcac gcaggagggc
    18721 ctggaagcgt tcagccgcgg ccggcagaca aacctcatct gtgtcgacgg gctcgatctt
    18781 tacgaggtac tgtccaggcg ggtttccctt atcgaggttc tcgaagaaaa ggcgcgtcgc
    18841 gccgcggaaa cgaaccgcgc atacattccg gtgcgcgact tgacgttcca ggcgcgctga
    18901 catggtcggc tcgggttcgg acgaaacaac cggaaccgga aaatggagtc agccgggtgt
    18961 cccgcacaag ggttggcgtt gcgtcgacat cgaggaacgc gacgagcaag accatctttg
    19021 tgagatgtgc gaggcgcggc aagtccgctt cgtccatgcg atggagcatc ccgcctatga
    19081 cgagatcctg cgcgtcggct gcgtctgcgc cggccacatg gaggaagatc tcgtcggcgc
    19141 tcgcaagcgc gaagccgcat ataagaacga tcgcagtcgt cgtgtacgct ggctacagcg
    19201 agcatggcgc atttctgcgg caggaaatga atttctcaat accaacgacg gcttcaatgt
    19261 cgtcatctat cccaagggat caatctgggg cggccgagtc gagcatcggc ccagcggcta
    19321 tcagcggact tcaaagctgc cttatgggtc gcccgatgag gccaagctgg cagcgttcga
    19381 cgccatgctc ggcatgaaac atagcgaacc ctggaagaag aaggcccgga cctaacttaa
    19441 gttggccgcg ttcggatcgg gtggtttcgc cccggggagt agtcgcgctt taggtgcctg
    19501 gcactccatt cgcgatccgc tcgacctcgc ggacggatga gaggagcaac gtctgaccgt
    19561 cgggatcgga tttcgtcgat ctcaacccgg ccggcgccat cggtggcgtc cgcgacctgc
    19621 gcgccgagaa gctcgtagag cggacccgtg atatagaccc ggcatgcgtc tcggatcgca
    19681 tcgagtttga cgcggtgtga ctgatgaagg gcgcttttcg aggctcgtcc tgcgatgcag
    19741 cggtagccga tggagaggaa gacggagcgc tcataaaacg ggctccgccg gaggttccaa
    19801 acggatgtgc aggtcggcca gatcggcgac gtccatcccg gctcccgcca acgtctggag
    19861 atagtccagc agatcgtcct gccggggttg gccgaaggct agacgataaa cagtgagact
    19921 gcgtttgagc cacgccaggc gcgtcacttc gcggctgaag gggagcatcg gaacgcgccg
    19981 ttcgacacga acggagccct catagatcca ataggggatg aggtcagtgt caacggtggt
    20041 ttcggatcgg gcctgctcga acatctgcct ccaagggtca tccggcgtgt ggccattgcc
    20101 ccggagtgcg tacacttggc gttccgccag attgaggcgc acggcgtgtc ccttgaagcg
    20161 gtgaacgcgc ccttcgcgct gttccaggtc gaccggattg cccggaagat tccagtggta
    20221 gacccggtag caatacgggt gaaagtccag cccctcttgg ccgatggagg tagtggcgag
    20281 aacgaagggc cggaacgggg aattgaacgc gtccctgacg ccgccgaggc gcgcaacggc
    20341 gccttcctca tcccgataat cggccaagcg catcgcgaaa cggccgcgca tctgaaactt
    20401 cccgatcgtg agagcgtcgc ctttgacatg gacgtcatcg acgtcgatct gcgagggtcg
    20461 gatggacatg gccgtagcca tcgcgcgggc gacacctccg gcccgttctg ctggtggccg
    20521 ggcacctagg ccctccgcgt cgacaagata gtgcgcatat tcgtccagaa cggcctggag
    20581 gttctggtcg acgccatagg tcagaacgcg atgccagtac cgatcttcgg cgcctcgccg
    20641 gagcagggcg atggcgtcat gctgattgaa cagcgtgcgg aacgcccagg cgacctcggc
    20701 ggcagcgctg agcaagcgcg gatcgtccca agcaagctcc ggcgcaatcc tgcgtagagc
    20761 gcgcagcgcg cacaccgccg gactccccag cgccacgtca acgagcaggt cgcgaagatc
    20821 gtcagataga gcgccgaagt cacgttgctt cgcggcgctg ctcaattcgg cgacgtgctc
    20881 ccgaaatccc tcctcactgg ccaaggcggc catgcctccc ggagccgaga gccaagcgcc
    20941 agcacgtgag cccagcagat cgtcaatcac ggcaggggcg gtccattcgg caacggcagt
    21001 cccgccatcc tgcgcggcaa cttgcctgag cgcgtcgagc gccggctcga ggcggtcggc
    21061 aatggccgcg cgcatcgctt cctgcgacag cctttcgttc tgcccggcga agacagagag
    21121 cggatcggcg atctcggcca atttagggga cggatagatc agcaggagcg tccgcagtcc
    21181 ggccaaccgg ccctggtcct gccgaaactg caacggccgc aggcgatgat gctcgaaata
    21241 gcgccggcct gattcgccga cgcccatgcg ccgttccgcc tcataggaca gaagcgcggc
    21301 aatggcgtcg ggcaccatgg accaggagga aaagatgagc gccttggtca gcggcggctg
    21361 cgatctctgg gagccatagt agggcatggc tgccgggatc cagaggttct gttcaaggcc
    21421 agcgccgaat atgtcatcca tgatcgcacg catgcgaccg ttggctggat cgagcggagc
    21481 gtaggcgtcg agcgcgtcgt gatcgagcat ggccggccgc gcggccagaa tcgcgtcgcg
    21541 aagcgctgcg gatggcgtat tcgttcgctc ctccaataag cgcttcaagc tgtagtgtcg
    21601 catgaagttg agaagataag gtgctgattt ccaatattcg gtgatctccg gggcatcgag
    21661 cgcacgcgcc acattcgaga ctgcggccgc ctgcgcgaga tcggccggtg cgaccgagac
    21721 ggccacgggc ggctcgccca ccatggaatc gcgctcgacg gtgctggcga cgcgctcggt
    21781 gcgcgcaatt acccggcgca aacgctgctc gaccgtcctg cgcgcctcga ctgcggcggc
    21841 atgtgacgcg ggcaagccat gcaagaaccc tcggaaggcg cgcatctcgc gcgccagcgt
    21901 cttggctacc tccggccccc tctcgcggcc gtagagaaag ctcaacgtct ccaggaagtc
    21961 ctgatagtga tcgccttcgt cgggctcgtc gccggcaagg gtcagcatgc gatagggcgt
    22021 cgcggacagg agcaaggttc tggcggcgtg accgtcgctg cctgaatagt cgaacagctc
    22081 gcgcgccagg atcgcggcgt cgctgtcgcc gtgcagcagg tcgcggaagc gctgaaactc
    22141 gtccatgatg atcagatcag gctgaagcgc atccacgcag gcgtgagaca gtttcgcgcg
    22201 caaccttgcc acgaggctgt tgcgcggttg ggtcatctcc cacggatacg cgtcccgccg
    22261 gcgcgggaac atctcgcaaa cgcgctccaa ctcctggaag agatcgcgat cagcctgcac
    22321 atcgcggcgg aagcgttcga tgatgcgcgt gtcgacgccg cgcaaatcca agctgtcgac
    22381 ggcccaattc cagccgtcaa cgccggcggt gacctgcagc aggttgtgga gcccgcgcgg
    22441 acgcgagacg tgatccttca gcaggtgcag caacagcgcg cgctcttgcg ttacgccggt
    22501 cgccgatcgg agatcgaatg tggtgcccgg agtgaggctg atgaagttga ctttgttggc
    22561 gtcgagtccc tcctggtccc gcagttgtag cggtatcagt gtcattcggg tcggcagggc
    22621 cagttcccgc cggccgagga cgttcagccg gtttaggttt tgtgccgcga tcgcctgatt
    22681 cgagcagatg tagaggatgt cgatccgctc gacgctgtcc cacagatgct caatcattcg
    22741 ggcgatgacg ccgcgggcga ccatggtttt gccgagcccc acctcgtcgg cgaccaggaa
    22801 ctggtggaca ggatcatccg ggccgtagag ccggtcgaaa acgtagtcga cggtgcggcg
    22861 ctggaacgcc ttcaggggcg ccaaggcggg cgcggcgcgg aaccgttcat tggacatgcg
    22921 cgccctccgg aactttgagc gcgacgcgga atgtctccca aagggtgcgg aactcggccg
    22981 ggatggggtc ggcttcgcct ggctcgacgg tttccaatcg ggtgatcagc cgttcgatag
    23041 cgcggagctg atcgccgccc cggcagaagg cgcgcaccat ctcctcgagg agcggcgcgt
    23101 catcggcggc catgcgccat gcgccgtggc cggagccctc ttgtgcggcg agtgcggcgg
    23161 cgaacgggtc gccaagttcg gagagtagca gccgcaaata gcggaagaag gcatccttgc
    23221 tgtcgatcac ccagcgcagg attgcggcgt gccgttccgg aggcaggcct tccagaagga
    23281 ggccggtgct gaacagaagg gagacttcgc catccccgtc gaccagatgg aaagcgagga
    23341 agtgtgtaag atcgatcagc ggcattgcgc cgagatcaat cggctgcccc agccgcagcc
    23401 tctcaagagc atcttggcca tggccttcgc cgcgcgtgat gggccatatg cgcaagccgc
    23461 cgatgccggc gagcgggagc ggtgtggaag ggatcagcca gacgcgccag agcagactgt
    23521 cgtcatcggc tgattgggtg cgctcgcaac gaagcttcaa gccgctgcaa caaatgtcgc
    23581 ggcgagcggc atccaaccgc gcttcggctg cccgcagggc tgaatcgacg gcgaccagtt
    23641 cgttcggcac gaagggacgt gtgagccgac caaacccctt ttcgccgaga atctcctcga
    23701 tgctgccgac acgtgagcgc ttgccggtga gcgtggcgaa gacctcgata ttgcttcccg
    23761 acaaaagagc ggggcgggtg gcgttaccgg atccgaccgt gatcgttgtg tcccagccca
    23821 tctccgtgat gaaggccttg gcgtgcaacc cctggagagc ggtcgcgtct acttcctcgc
    23881 catcctcggt ggccgccatc tcgtccatca ccgccacccg ggcgaaacgg ccgagcgttg
    23941 cagcagggac cagcgcgagt tcatccgagc ggccgatcag gacgggtttc tcggcgccgg
    24001 cgagatctgc gagcattgac agtgccgtgt cgtcgcagaa cggcgagatc acgccgagcc
    24061 gggcgcaggg ttcgggacgc cattgcttgc ctcccagacc attcacggcg aacgcgaggc
    24121 tttgaaaggg ttcgggcaag ctccaatcgg tgcggcgcag gtcctcggca atttcctcgg
    24181 tgaggtcgcg agccccctcg ggcagaccgg ccgtcgcaaa gtccggcagg cggcgaatga
    24241 gatcggccaa aggtcgattg agggcctgcg gccgtcgcgc gaccacgccg tcgagcgtca
    24301 acgcgatatc ccacgagcga tcgcgggtga gattgcgcga cagcaccagc aggcgaaggc
    24361 gagtcggttc gtcggagcgt accggcttga agcggagcgc ccatatcttt ggatggaaag
    24421 cgccgccgct gggcgccgcg acctccacga tgatacgttc tagcaacgag cacaggcgag
    24481 aatgcggtcg cgtgtgcgcc tggatatgtc cggcgtcagt gaagacaagg agacggccgg
    24541 cgatgcgctc ggctccttcg agcagcgcca agggatgagt cagaatgtcc tcgcgattct
    24601 cggcggcaaa cagcgccagg ctgaccggca ccgcgagtgc cgtctcgaag tcgagcgaga
    24661 acgtggtggc gacggcggcg tcgaagatat agccggccgg cggctggagg ttggcgccgt
    24721 agagggtgcg ctgttcggga tcgaggctgc gcctattcag catgagccag gtccctcaaa
    24781 tgcgatctcg cctggttcca gcgaaagttc aggcggtcga cgccggaagc accagtccag
    24841 cgatcgcgga cggcgtggtt ggcgtagcgc gattgactgg ttttcaatcg gcgttcccgc
    24901 tcttccacga gttgtcgggc cgcaggcgcg gaagcaatct gcgtggtgcc atcgaccacg
    24961 agttcgagcc actgcgccac gaagcgcttg gccgcgggcc ggatgctgtg ggcgggatgt
    25021 tcgacggcgt cccagaaatc gtcgagggac cagttgcgaa cgacggaaag atccagctcg
    25081 gcggcccagg ccgcaatgcg ctcgcgatag tccgcgaccc aatcgtcacg accacgcaat
    25141 tcgctgagcg cgagattgta gaggagcgcc gccccatgca tcacatgcga gaagatctcg
    25201 ccgtggcgga ccaggcggcg cgcgtccgcc ggaaaggatg ccagatgtgg atgggtccag
    25261 atatggtcgc attccgcatc gataccgtca cgcgccagca tggtcagtag cgccgccggt
    25321 tgggtcgcaa ccaggcgatc gatgatgaac tgcgcttcgt ccgcggtcag gctgaaggcg
    25381 gcgcagtcga gcaggccgtt gggcgcctgt ggcaaggctg gattccagat agctgccgcc
    25441 cgtgaacctg ccgcggcgtc ctcgctgtcc gacgagacgc gcggccactt caggccgcgc
    25501 agagagatga aaaggctatc tatcgagccc gggaagaggc ggatgcccca ggcgccgagc
    25561 ccggcccaat agactgagct gggcagccgc tgcagccttg gaccggcgtc gcggccgatg
    25621 atgccattcg actctccgcc agccttcagc gcgtcggcta gccgaatttc cagggcgcgc
    25681 gcttcggtgc gaagctggct ttcgggaacg tcccgacctt ccagcgtgcg gaacagccag
    25741 gggatgaaga gcatataccg caagcgggtc tggatcgtgc tcgtccccgg aaaaaggtgg
    25801 tccgcgatcg aatcccggat ggcgcccagc cccagctcgt cgcggctttc gcgctcctga
    25861 aagagagcca ttatccgctg cgcgcgctgg cgctcggctt catcgaaatc gatccaggca
    25921 agagatgaca tccgcgtcgc gtcccgcttt caggcaaccg cgacggccgg gttcgtgcgt
    25981 ccgacgggga tgattgcgcc ggaggtcacc agcacgatca gcccgcgttc ggcttgggtc
    26041 atatccaaat aggcgcgcac ttgagtgcga tagtggtcga gcgtctcggg cgcgggcgcg
    26101 acgtcgctct tccaatcgac gatcacctga ggcgcgccat cggggccaaa ggcaatcgca
    26161 tcggcgatcc cggccgtcgc ttcctcctgc tcttcggtca agatggaggc gtagactgga
    26221 aattccggca agagaccggg ccgcagagcg acgatctccg gcaaagcgag agcgcgaaga
    26281 acacaagcgg cgagttcggc cgctgcaaga ccctgagcag ggtcaggggc caccggctgg
    26341 ccaatggcgc ggatcaaggc ttcggcgcgg gcaatgagag cgggctccgt ctccgctgtt
    26401 tcgccggtga gaacctcttc gatgagcttg tgcagaatga cgccgcgctc gcgaccacct
    26461 tggatcgtct ggacgatgct gtcctctaca ggcgcatcgt cgacatcagt caccaggatc
    26521 tccggtagct cggcatgcaa cgccggtccg gtggcattct cgtcgcggct gggggcgagc
    26581 cagacaatgc gctggcggcg ctctgcgatc atctccgctt cggccgcgaa gatttcgcgg
    26641 gtctgggcat tctgcgcgcc agccccatcg gacccgacct cgggcggcag atggctaaga
    26701 tcgagcgccg gcagttccgt cagcgacagg tccagaaggg aaatccaggc cgatcccttc
    26761 gcagcgacat cgagcctcgg cagaaccagc agctcgcgcg cccgcgtagc ggcgacatac
    26821 cagagacgga tgcgctcgcg gtcgagctcg gccttctctg catcgcgcgc cgcctcgtaa
    26881 cccgtcggct tcacgcccaa caccggacaa tagaagcggc cggtggcacg gtcggtgatc
    26941 gcgctctccg gcgccatgat ctgcgtcatt gtgttgacgg ggacgacgat cggccattcc
    27001 agccctttgg cggcatgcat cgtgtagagc gcaaccgcct cctcctgagc atcggggcgg
    27061 ccttcgacgg cgcgggcctc gtcggtccac gcggccgtca ttgcctccgc gaaggcacgc
    27121 aggccgcgga ccgcatacgc ccggctgagg ctaagataaa gatcgacatt ggcgagggca
    27181 cgctcggcct ggtcgcgatg ccgatgaagg aggatcggcc gcacgcgcag cacgtcgatg
    27241 gcctgcgaca gcaggtcgtg cggcgtcgtg gcgttcatcc tcagccgcag cgcctgaagt
    27301 ttctcgatga tgtcgcgcgc atgcggatga gtgatcgttt ccggagccac gccgaggtcc
    27361 agtcgcggca aggcgtcggg cgcatcttcg gagcgcggga gcgcccagac aatgtccagc
    27421 aattcttcct cagtcagccc gaccagtgga ccacgaagca gcgcgcccag cgcgagcgtg
    27481 tcgcggcggt cggcaagcac tcgcgtgagg gcgatgaggt cctggatctc ttggcgccgg
    27541 aacagtccct tgccggcctg agtcgcaact ggaatgccgc gccgctccag cgcttcttca
    27601 tagcgccaca gatcgcttcc cgtcggcgcg agaagagcga tgtcaccggg ccggcaggcg
    27661 cggcgctcac cggttttgcg atcgagaatg gcctcgctgc cgatcaggcg cgcgcacatg
    27721 tcggcgacag cctcggcttc gccgtcgcgc tggcgttcgg ccgaggcctt gccgtcggca
    27781 tcggccacgg caatgtcgag ggccgcgacg cacagtccgt cgttccgttc aggatggaac
    27841 gcatcaagag cggtgaaccc cggctggccg ttctcggccg acagcaggag ttcgaagcga
    27901 tcgttcacat aattcaggat cggttcgcag gaccgaaaat ttgtcgagat cgacacgacg
    27961 ctgtcgggtg cctgcgcaag gaaggtttcc cgcgcctgga tgtaggccgc cacgtcggcg
    28021 ccacgaaagc ggtagatcgc ctgtttgggg tcgcccacca ggaacagcgc tcccggccgg
    28081 atttggaacg tggtccaatc gtcgcggtct gctccagcct gcgggtcgcc gcacagtcgc
    28141 cagaagattt cggtctgcaa agggtcggtg tcctgaaact catcgaccag gacatgcgca
    28201 aagcgcgcgg ccagcgcgcg gcgcaccgct tcatggtcgc gcaacagggc gcgtgcggcg
    28261 aagatgaggt catcgaaatc gagaagcgca gccgagcgtt tgtagtcacg gaagcgatcc
    28321 atgaccggct gcactagctg gacgaattcg gcgaggatgc gcgacgcggc cgcctgcgac
    28381 agtgcggtcc atgcttcgca acaggcattg tagtggcctt ccgcgccagc gttcaagcga
    28441 tcgccatccg ccttggcgag gcccgcgtgt ttggcggctt cgcaccactt gcctttcttc
    28501 ttgtaggcgc gaaagcttcc tccggccgtg cagagatcgg gatgggcgcg cgcgagcaac
    28561 agccgcacca gatcggccgg ctccgccggc gagattgcct caatggcttc ggccttttcc
    28621 aagaatagcg tcgcgatgtc agcggtctcc ggctcatcga ctccggccgt gtgcagccaa
    28681 gatacgaagc cgtctgctgt ctgccggacg gctccgatga gcggttccag cgcctcgggc
    28741 gcctcaacag ccagcgtgcg acggctgcgg aggtgcgtga gaatcttgtg gatcagccag
    28801 acggtctcgc ccggatcctg caggaccatt tcggcaagca gtccgccagc atcgtcggca
    28861 agttcctccc gaagccagat ttcaacgatt tccgcaaagg cgaggtccgc ctggtcgcgg
    28921 tccatcacga ccgccccggg atcgatgtcc gcctcgacgg gataaggctt gatcaggcgt
    28981 tgacagaagc cgtggatggt cgagcaggtg atttcatcga tcgccacgct ggtagccgcg
    29041 agattgcggc gttggtcttc ggacaagcca tagggcaagg caacccgaag ctcggcggga
    29101 atgcgacctg ccaccaactc gccaacgaag tcgcggacac ggatgagaag ctcgctggcc
    29161 gcgagctcag tgaacgtcac cgcggcgatc gtccttggcg caatgccttc ggccagcatc
    29221 agggcgatgc gaccggccat gacggcggtc ttgccggaac ccgcgccggc ttcgaccaag
    29281 aacgagcggt cgtgaaccgc gatcacagtg cgccgcgcca catcatcggc gagcggtttc
    29341 agcggggcat tcattactga gcctcccaga cctgcgcggc gtccccgagg cgctcggtgg
    29401 cggcggcaat cttgcgcttg caataggtgg cgccggcgtt agccggcaat gcgatggcga
    29461 ggtcgtcata tgtcccgccg gtgtcggccc cgagtacggc gccgccggca aggagactgg
    29521 cgtgcgcggc ggacagatag ccggcgacgt ccgccaaagt gccgtcggga tcctcgagcc
    29581 gcagatcaat ctcatcgcgc ggaaagagca gggaggcgct gatggcgaca tcgtcgccga
    29641 gcatcgcctt cacggcgaag gcatagagac agcgctgcag ctccttgccg ccgtcgagaa
    29701 tgatggtgtc cttgggctta cggccggtct tgtagtcacg cacaagagcg cggcggccgt
    29761 cggcggagac atcaagccga tcgatgtagc cggcaatccg gaagtcagtg ccaggaattc
    29821 cgacgctggc ggtggcgtcc cagggcagcg cgtcattggt cttgggctcg gcgcctccga
    29881 aagggacctc gccgaaggcg cgggcatcag gcaggcgctc gtcgccaaag gcgagggctc
    29941 gccggctcaa ctcccgcgcc tcgtcgaggg tgcgccgcca gattacgcgc ggcggcacag
    30001 gacgttcggt ttcccagctt tcggcaacct cgccggcagc gccatcgacg gcgctggcga
    30061 tctgttgccc cgttgcggcg gccagtccgc cattggcttc gagtgtgcgg agagcccgat
    30121 ccagaatctg gtgaacgaga tcgcccatcg cccgcgcatc gagaacgagc gggtcgtcgc
    30181 cgctttcggg cgcgcgccag cgcaggccgt agtgccagac gaagccgagc ggattgcgca
    30241 agagcttgcg aagggagctg gccgattggg tgcgcgccag gatggcctgg atcaccgggt
    30301 ggtcggcacg aacgagtccg tcatggggag taatctcctc ccgcagccag ttgcgccaac
    30361 aggcggccgc cgaaacggct tgcgggagcg cgcggaattc ctgcggccgg gcggtcagcc
    30421 ggtcggtttc gctgaaggcg tgatccggca cccggttgcg ccggagataa acctcatcgg
    30481 gctggccctg caggagggtg ctgcggccga ggaggcggcc atcgctgtcc cgtcgcgccc
    30541 gcgacaggac aacctggcgc tcgctggtcg cgaggattgt tgcgaaatcc cgccggtcgg
    30601 ccacgccgac agggagcggg tcgagctcag ccgtggggat gatgtgatcg gagaggaggc
    30661 gatcttccga aatgccgcgc ggccaacgcg aggaattgag gccgaggagt cgaacgaagc
    30721 ggcgcggcga tgccgccaac gcgcttgcag gcatccatgc gaccgagacg caggcatcca
    30781 gaccgtcgtc ctgcttcaac gtctccaggg taagatcaag cgaggcggcc gggccggcaa
    30841 ggagcgcctt gcgccagatg gccaaggtcc tgccattcag cagcgcttca ccaactgctt
    30901 cggccgcagc tacgccctgc gacagcagac cgataatctc gcgcaacgcc ggaccatgat
    30961 cgatttcgtc gggccagtcg gcagcggtca ggcggtcgag caggtgggtc caggctcggg
    31021 gcgacgccag gggagcatct gccggcagga cgcgcgtcca gcccgctggc aaagcctgaa
    31081 atgcgccgga ataagcggcc agcagggacg acagacgccg catccgcgtt tgagaaagcc
    31141 cgcgcacaag gatgtccgcg agcgcggcgg ctgcctgacc ttccctacag gccgtgatct
    31201 tgacgccatg cacaaagtga aggtcgagat tggcgtcggc ccgcaaagcc aggaaatggt
    31261 cgtcgtaatc agccggcgtg acggatgcga tggcgatctc ggcgggatca gccgcgccgg
    31321 acgcgacgag ctgccgtgcc caacggagcg cctcgatggc ttcgtgatag gcggtcgagg
    31381 cgctgacggt gacaatctct ggcgtgtgcg gatcactccg cgtcacgtcg ataacagctc
    31441 cgtcgagcca ggcaggcgtc gaccgcggac cggcgctcca ggtaatcggc acgtgctgag
    31501 cgatagcgtg cagaagcggc cgccagcacg gcgacagctc ggtaattccg acgatctcga
    31561 tcggcccgaa gagggcgtcg acatggtcca gccgctgcaa ggctgccgcg accaaatcga
    31621 ccggacgcat catggccggc ggcaaggcgg caagcactgc ttcttcaagg ctggcgagag
    31681 atgcaatacg tgggtgctcg ctggcacgcg cctgcaagtc gacgcccgcg cgccacgcct
    31741 tgcggagcgt ttcagtggcg gctcccacca tgcctggcag agccttgagg ccatcaagct
    31801 cgcctagctc gacgtccggc agcatccgct ggatggtggc gcgcaatgtc tcgtcgtcga
    31861 ccggacgcga caagccgccg gccaggcgcg cggcgagccc ctcgaaggtt atgatctgga
    31921 ggccatgacg gcgctcacgt gcggcggcga gccgcacttc acgcatcgct aatcgtccgt
    31981 gggcgacgat cgtgcgccgt tcttttggtt gcattggttg cccccgcgct aaagcccgtt
    32041 gcatcgcttc tggtggatga aggcggcgcg ctctttgacg cgccgcaatc caccttgacc
    32101 gatgcccccg atatgaatag taattacacg ggtcgcgtcg atatgtccat atagctttta
    32161 tgcgtatcgg caaacggagg tggagcgatg gatgccgctg tggcgctgaa atggggcgta
    32221 gagcgccggc tcgaattcat cgagttccgc ctcttctggg acgggggcgt gaaccgctcc
    32281 gacatcattg atgtgttcga cgtgtcagtg cctcaagcgt cgaaggattt gacgctctat
    32341 caggagcgag cgccacacaa cgccgtctac gacaagagtg ctaagcgcta tgttgcgagt
    32401 ccacagttct cgccaatttt cctgaagcct gaagccgagc aatacctcaa ccagcttcga
    32461 tccgttgccg acggtatctt ggactcatcc gagtcttgga tagcgcatct tccgccctac
    32521 ggcgggccgc ctgtcccggc ccggggggtg aacaccgaaa cattgcgaac aatcgtcgcc
    32581 gcgatccgtc aaaaagaggc agtggaagta aagtatcagt cgctatctag cgatgatccc
    32641 aggtggcgat ggattgcgcc gcatgcgatc ggctttgacg gataccgctg gcacacccgt
    32701 gccttttgtg aggtcgatcg cacgttcaag gatttcctgc tgtccagaat cattgagacc
    32761 agatcacttc gaccgagcga ggtatcggct gagcatgaca ccgactggaa cgaattcgtc
    32821 acactggaaa tagggccgca cccagacctt tccgaaagcc agaagcggat aatcgccctc
    32881 gactatggca tgcgaagtgg gcgcgcacag attcgtgttc gaaaggcgct gcgctactat
    32941 gctctgaggc gattgggtct cgatacgcca ccggccgctc gtcgcccgca agaccagcaa
    33001 atcgtgcttc tgaatgaacg gggtgtcgat gaagatcatc gataacacct cgcagctgtt
    33061 cggtgacgat ctgaaagcga cagtcgggcg tggcgcccga ctgaagatcg ctgcgtcgtg
    33121 cttctcgatt tacgccttcg aggcgctcaa gtctgagctt tcgaagatcg aaagccttca
    33181 gttcattttt accgcaccga cgtttgtgcc gatggaggtc acggaccgcc tgcgcaagga
    33241 acggcgagag ttcttcatcc caaaagcgcg gcgggagagt ggcctttacg gaacggagtt
    33301 tgaaatccag ctccgcaaca agctgacgca aagggccgtg gcgcgcgaat gcgccgactg
    33361 gattcgcaag aaggccaggt tccggtcgaa caccacgaaa gctccgatgc agcagttcct
    33421 gcatgtggcg agcgatgacg gcggaattgc ctacatgccg atcggcggct ttacggccgt
    33481 cgatctcggc taccagaaag gcgacgccat ctcgaatttc gtgacgcggt tcgacgaccc
    33541 gaaccacact cagatgtacc tccagttgtt cgaccaaatc tggtcggacc cggagaagct
    33601 cgatgatgtc acggccgcga tctgcgacca cattgagtcc gtctatcagg agaactcgcc
    33661 ggagcgcatc tacttcctga tgctgtacaa catcttccag gacttccttg aggatatcga
    33721 cgaggacgtt ctgccaaacg acctcaccgg gtatcgtgac agcgtcgtct ggaacaagct
    33781 tttcaactac cagaaggacg ccgccaccgg cattatcaac aagctcgaaa cctataacgg
    33841 ctgcatcctc gccgacagcg tcggtctggg taagacgttc accgcgctgg cggtcatcaa
    33901 atattacgag ctgcgcaacc gcgccgtcct ggtgctctgc ccgaaaaagc tcgcggacaa
    33961 ctggcggaat tacaacacca acctgacgac taacatcttc gccaaggacc ggttcaacta
    34021 tgacgtcctt tgccacaccg atctgtcgcg gacatcgggc gagtccttcg gcattccgct
    34081 caaccgtgtg aactggggga attatgacct cgtcgtgatc gacgagtcgc ataatttccg
    34141 gaacaacgat gtcttcaagg atcgggagac gcgctaccag aagctgatga acaaggtcat
    34201 ccgcgcaggc gtgaagacca aggttctgat gctctccgcg acaccggtga acaaccgctt
    34261 caccgacctg cgcaaccagc ttgcgcttgc gtatgagggg cagtcggagg ccctcagcca
    34321 gaacctgaag gcacaaacca gcatcgagga aatcttccgc cgcgcgcaga aggctttcaa
    34381 cgcctggacg atactgccgc cggacgagcg aactgcggcc tcgatcctca aggcgctgga
    34441 tttcgatttc ttcgagcttc tcgatgccgt gacgatcgcc cggtcacgca agcacatcga
    34501 gaccttctat gacacgaagg acatcggcaa gtttccccag cgttgcaagc cgttgtcgtt
    34561 tcactgcccg attacgcagc gtccagacgt gatgggcctg aatgacatct tcactcagct
    34621 atccgtgctg aagctcgcgg tatatgcgcc catcagttac atccttccga gccgactgcg
    34681 gaagtacgag gagatttacg acacccaggt cgagggcggc cggggcaagc tgcggcaagc
    34741 cgatcgtgag cgcagccttc aggcgcttat gaccaccaat ctgctcaagc gcctcgaaag
    34801 ctctgtcgag gcgttccgtc tcaccctgcg ttcgctcagc gggaacattt cgcgcgccct
    34861 ggacgccatt cgtgacttcg agcggtcagg cggcacgcaa agcgtgagcg attttctggc
    34921 ggacgaggaa ttgctcgacg ccgaagacga cgatctggcc gggctgggcg acatcaccgt
    34981 tggcaagaag gtgcaaatca gccttgccga tatggacctg ccgtcatgga agcacgatct
    35041 cgaggccgat ctcgcgctca tcgaggacct gatcgcctcg atggacaaag tcggcccgga
    35101 cgatgacgcc aagctccagc acctcatgca ggtgctcggg cagaaggtga gcgaaccgct
    35161 caatccgggc aacaggaagg tgctgatctt cacggccttc gccgacacgg caaattatct
    35221 ctacgccaat ctggcaccgt tcgcgcagca gctggggttg cagaccggaa aggtgacggg
    35281 ctccggcgcg ccgaaaacca ccctcagaaa gagttatgac tttcagggcg tgctcacact
    35341 cttttcgccg cggtccaagg agaaggcggt cgtcctgccc aacgaaccgg gcgagttgga
    35401 tatcctgatc ggcacggact gcatctccga aggccagaac cttcaggact gcgattacct
    35461 cgtcaactat gacattcact ggaatccggt gcgcatcatc cagcgcttcg gccgaatcga
    35521 ccgcatcggc tcgccgaaca cccaaatcca gctggtgaac tactggcccg acatcacgct
    35581 tgacgagtac atcaatctca aggagcgcgt cgagaaccgc atggtgattg ccgacgtgag
    35641 tgccaccggc gacgacaacg tcctcaccgc caagagcagc gacatcgcct accgcaagga
    35701 ccagctgaga cgccttcagg aagaggtcat agagctggag gacgtgaaga ccggcatctc
    35761 gatcaccgat ctcggcctga acgattttcg gatggacctg ctcaactacg tcaaggagca
    35821 cggcgagctt gataacctgc cgaacgggat gcatgccgtc gtccccgctc agcccgatct
    35881 gggtcttcgg cctggcgcga tcttcgcgct caagaacatc cacgacagtg tcaacatcaa
    35941 ccagcagaac cggttgcatc cctactacct ggtctacatc ggcaatgacg gagagatcgt
    36001 cgccgaccat accgaggtga aacgcctgct cgacctcatc cgcacaagct gcaagggccg
    36061 gccggagccg atccacgaag tctgtcgcgc cttcaatgag cgcacgcagg acggccggaa
    36121 gatggatcgc tactccgagc tgttgagcag cgccatccgt tcgatgatcg aagtgaagga
    36181 agagaaagat atcgacagcc tcttcagcgg tggtcgcacc acggcgctgg tgcataccat
    36241 tgcggggctg aatgactttg agctgatcgc ctttgtcgtg atcgaggggg ccgacgcatg
    36301 acagccttct tcgactatcc caagagcgcc gccttcggcc gggtcgtgcc gaagaacaag
    36361 atatacgagc atgccggcgc caacaccgcg ctcaaggatc tgttcgtgcg cgaggttgat
    36421 cagattctct ggcggttcaa gctggccccc gagacgacca atctcggcgc tacctcggcc
    36481 gtgaccgaga tccaggtgtt cgggatatcg ctcaagaccg gacgtctcga cgaggccgtc
    36541 ctgcgcgcga tcgacaaggc gattccgttt cccctgatct tcgagctgac ctggcgcggc
    36601 aagcgcaagg cgacggccgc gttcaagcga ccgagcgagg cggacgggtc gaagtgggtg
    36661 atcagcgagt atttcgccac cgaatgggcg tcggaggatg caccgcgtga gccgttgccg
    36721 gtcgcgctcg atctgggtgc gctctatgac cgccttctca ccgccatgat gcccgcgtcc
    36781 gcgcaaatca gcgagcacat acagacgcgg gtggaccgga tggaggcgat ccgagccaag
    36841 acacgggagc tagagcggat caaggcgcgg cttgcccgcg agaaacaatt caacaagcgc
    36901 gtggcgatca atgcggagct gcgcgctgcg aagcaggaac tggagcggct gaccgcgggc
    36961 gatcccgtcg gcgctgtggc ggaaaagtaa gaggacaagg atggaaaagt tgaagatgca
    37021 cagccccgtt ttgagccagg aaaacctcgc gaacgtccgg gcgctgtttc ccaactgcgt
    37081 gacggaagcg cgcgatgagg ccacaggcaa gctgcgtctg gcggtggatt tcgatcagct
    37141 gaaacaggag ctgtccgatt atgtcgtaga agggccgcaa gagcgatatc ggctcgattg
    37201 gccggggaag cgggaggcgt tggcgctcgc gaacgcagcg gtcgcaaaga ctctgcgccc
    37261 ttccgataac gagagcgtgg atttcgacgg gactaagaac ctgttcattg agggagataa
    37321 ccttgaggct ctcaaattgc tgcaagaaac ttatcttggt cgggtaaaaa tggtctacat
    37381 agacccgcca tataatactg gaaacgattt catatattca gatgacttct ccgaaagtgc
    37441 tgagtcattc ttagcgcgat cagagcagag agacgagggc ggaaatcgac tagtagcaaa
    37501 cacgacgtcc aacggtcggt tccattcgga ctggcttact atgatgtact ccagactcaa
    37561 gctggcaaga aacctactct ccagcgacgg agtgctattt gttagcatag acgacaatga
    37621 ggctcacaac ctcattcgac tcggctgtga aatattcgga gaggagaatt tcgttgctca
    37681 gctagccgtc atggtaaacc cgcgcgggag acatcttgat cggtttgtcg cgaagacaca
    37741 cgagtcaatc ctcgtatttg tcagagatgg gctcaacgcg ggcgcgatcc agggcctcga
    37801 aaaagagggc cggatggtcg aggagtacaa caaatcggat gataaggggc cttaccgact
    37861 actcggactg cgcaaccgga accagtcatt caacccaact actcgtccga acttatacta
    37921 tccgctttac gtgaaccctc tgacgaggga agtttctata gagcaagatg accgctttaa
    37981 ggttgaagta tggcctgatg cgccagatgg agtgaagacg tgctggacgt gggggaaaga
    38041 gaaggtgcga aatgaaggat ctcttctttg tgctgagccg tatggagacg agtggcgcat
    38101 atatagaaga gattacctaa atggcgatga cggggaaacg tccaggagct tggtgaagtc
    38161 cctttggact gagaaagaat tcactaatga ctacggaagg aaggccataa aggacctttt
    38221 tggctccgca ttaatggact ttccaaagtc gccagacctg atttcccgcc ttgtaaagat
    38281 tggtaccacc cgggaatgca tcgtactcga tttctttgcc ggatcggcaa caaccgcggc
    38341 ttcagtcctt tcggttaacg ccgaagacgg cggcaatcgc gcgtttatta tggtgcagat
    38401 gccggaggag gttgagcaat cttccgcagc atacaaggct ggttttcaca cgatcggaga
    38461 gcttgcgaag gaacgcatcc gccgcgccgg agccaagact ctagagggcg attgccaccc
    38521 tgaatggaac agggatgtcg gtttccgtgt gctgaaaatc gatacgtcga atatgcagga
    38581 cgtgtattac cgcccggatg aggttgacca gaaggatctg ctcgaggccg ttgataacat
    38641 caaaccggat cgcacggcgg aagacctgct gttccaggta ctggtcgatt ggggagtcga
    38701 tctgagcctg ccgatccggc gcgagagcgt gcagggcaag acagtattct tcgtggacga
    38761 gaacgcgctg gttgcctgct tcgaattggg cgtgaccgag gagctggtga aggaactcgc
    38821 cggccgcgag ccgttgcgcg tcgtcttccg cgacaacggg ttcgtctcgg acgccgtgaa
    38881 aatcaatgtc gagcaggtct tccgccagct ctcccctggc accgacgtca agtcgatctg
    38941 agggggcggg catgaagctg aaattcaagg tccagcccta tcagaccaat gccgtggaat
    39001 cggtcgtcga ttgctttgcc gggcaaccgt tggtcagtgg cgtcacctat cggatcgacc
    39061 ctggccggaa ggcgcaggcg agcgccttcg aagaagggtt caagaacgcc gacatcgcgc
    39121 tcaccgacct tcaggtgctg gcaaacatca aggaggtcca gcggaggcag aacctgccca
    39181 tgtccgacgc gctggtctca agtgccgggt gcaggttcaa cctcgacgtc gagatggaga
    39241 cgggcaccgg caagacctac tgctacatta agaccatctt cgagatgaac aagcgctacg
    39301 gctggtcgaa gttcatcatc atggtgccca gcatcgcgat ccgcgaaggc gtctataagt
    39361 cgctgcagat cactgccgac catttcaccg agagctacgg caagaaggcg cgcttcttca
    39421 tctacaattc caagcagctg cacgagctgg agagcttttc gtccgacgcc ggtatcaacg
    39481 tcatggtgat caacatccag gcgttcaacg cccggggggc cgacaaccgg cgcatctatg
    39541 acgagctgga caatttccag tcgcgcaaac ccatcgacgt gatcgccagc aaccggccga
    39601 tcctgattct cgacgaaccg cagaagatgg aaggcgccgc caccatggag gcgctgccga
    39661 aattcaagcc gctgatgatc cttcgctatt cagccacgca ccggacgcag cacaaccgtg
    39721 tccaccggct cgacgcgctc gatgcctata accagaagct ggtgaagaag atcgccgtgc
    39781 gcggcatcca aactcgcggc ctggccggca cgaacgccta tctgtacctt gagggtatcg
    39841 acatctcgaa gaagtctccg gtggcgcgga tcgagctgga ggtgaagctc aagtccggcg
    39901 agatcaagcg tcagctcaag cggctggagt tccgcgacga tcttttctct gagtccggcg
    39961 agctggacca gtatcgcgac ggctttacaa tctcgcagat cgatgcccgc aacgacaccg
    40021 tcgagttcac caacggtgcg gtgcttcggg cgggcgaagc gactggggac gtttccgagc
    40081 gcgacatccg gcgcatccaa atccgcgaaa ccatcaaggc gcatctcgac aaggagaagc
    40141 agctcttcgc ccagggcatc aaggtgcttt cgctcttctt catcgacgag gtggtgaagt
    40201 accgcgacta cgagcgaccc gacgagaagg gcgaatatgc gcgcgtcttc gaggaggagt
    40261 atgagcttct caaggcggaa tatctgtcgg agctcgcgat cgacaatgag gtctatcgta
    40321 agcatctcgg ggcaatcgaa acgtcgaaga cccacaacgg ctatttctcg atcgacaaga
    40381 aaaccaaccg gctgaaagat cccaccgttg gcgctcgctc ggtcgattcg gacgacgtgg
    40441 acgcctacga cctgatccta aaggacaagg agcggctgct atccttcggc gagccgacgc
    40501 gcttcatctt ctcgcattcg gcgctccgcg aagggtggga caatcccaat gtcttcgtca
    40561 tgtgcatgct caagcacagc gacaatacga tctcgcgccg tcaggaggtt ggtcgcggcc
    40621 ttcggctttc ggtcgaccag cacggcgacc gcatggatca ccccgctgtc gttcacgaca
    40681 tcaatgtgct cacggtcgtc gccagcgaga gctacaagga ttttgtcggc ggcctgcaaa
    40741 aggagattgc cgacacgctg tcgtcccggc cccggaaagc gaccgaggcg tatttcaccg
    40801 gcaagaccat cgcaaccgag agtggtccgg tcgagattac gccggccatg gcgaagcaga
    40861 tttatcgcta cctagtgaaa aacgactact ccgacgacgc ggatcaaatt tccaagacct
    40921 accacgaggc caaagaagcc gggactttgg cggacttgcc ggacgagctg aagccccatg
    40981 cggagcagat tttccaactc gtcgatagca tattcagcga cgcccaactg ccgaaggtgg
    41041 atgatggccg gaagcccaag accaatccgt tgaatgacaa tttcgagaag aaagagttcc
    41101 gggagctttg ggcacgtatc aaccagaagg ccgtatatcg cgtcgagttc gattccgatg
    41161 agcttatccg caaatgtgtg aacgcccttg acagccagct acgggtcacg ccgctccagt
    41221 acaccgttca gactggactc cagggcgacg gccttacgga tgatcagctg aaagctggcg
    41281 acggcttcaa gctcaccggc tcaacgaccg agcgcggtgg gtcggtccat tcactcgtga
    41341 gatacgatct tctcggcaac gtcgcggaaa acaccgaact cacgcgaaag acagtggccg
    41401 aaatcttcac cggggttgcc gccagcgtat ttggccagtt cagggagaat ccagagcatt
    41461 tcatttctga ggcctcgcgc atcatcgccg agcagaaggc cacgatgatc atcgaacggc
    41521 tcgcctatga cggcctgtcc gatcgctacg acgtggacat cttcacggcc aatcagaccg
    41581 gacaggactt ctccaaagcc agctcgaagc tgaagaacca cgtgtacgat tatgtcgtca
    41641 cggactcgga catcgagcgt cggtttgccg atcagctcga tgcaagcagc gaagtggtgg
    41701 tctatgcgaa gctccctcgc ggcttcctta ttccgacgcc ggtgggcgat tacaacccgg
    41761 actgggcgat ttccttcaag gagggcagcg tcaagcacat ctattttgtc gccgaaacca
    41821 agggcacgat gtcctcgatg aagctccgcg agatcgagaa caccaagatc gagtgtgccc
    41881 ggaagttctt cgacgagatc ggccggaggg tcacggagga tcgcgtcaaa tacgacgtcg
    41941 tgacgagcta cgagaagcta atggatattg ttgggcgcgc agcccactga tcgggctgcc
    42001 tgagacattg gccgcggaga gaaagccaag ctgactcagt ggcactgtga ccatctccaa
    42061 ggcgtcaatt cccgccttgg cgcgcgtgac gttccctcag caggtcccca acgctccgcc
    42121 catccacgcg gcacactgcg atgactcgat catgggagat gacgcgtccg ctccggccgc
    42181 ggcgagcctc gcattgcgct ctgcggccga acgcgatctg ctcaagtagc atctttgcac
    42241 ggcggccttc gggggcgtga agctcaggcg catcaaagtc ggcaagccgg acctcgatcc
    42301 aagtctgggg gtccggcgac tgaccaacgc agaggctgtc gccgtcacca acgtagcgta
    42361 tcaagccctc aaatttttgc ccggccgctg caggaaggcg cccttcgcac gggtcggcct
    42421 gtgccggcgc agcccaccag aatagcatga gcgcgaacgc cgctgatagg agatggcgag
    42481 cgaggcgagg cgtgccgact gggcgctctg agctggccgc catgtagcgg acgatccaat
    42541 tgattctcgg tgaaaattcc atcgccgatg cccgcgctag ccgagaccga ctttgtagcg
    42601 tgcgagaagt ttggccaatc tggtccggtt cgttgtcata ggctgaggat cgctcggagc
    42661 gaggtgatcg ccgacgtgct caaacagcgc gacacaaatc tcgccattgt cgtgattgga
    42721 gcggtaggca atgccgtccg gcaccgtagg atggtcgtgg atggcttgcg accactgttg
    42781 ggcgacggca tgaggcccac tcgtgacggc accggtcgcg ccgatctgcg ctaagccggg
    42841 gccgtgaagg gaaaccaagc ggaggggcgc agagacctct atctcggaga aggatcgcgc
    42901 ctccaggaaa gtcagggcaa caaagcgtgc gccaacgctg cgtaggcagg tttccgcaaa
    42961 ggcaccttca agcgtcgtcg caagatagca gaccccatag atctttgccg gatcgtcgaa
    43021 gcgattgccg cctgtcgagc caaaaaagag cgggccgcgg tcgctgcggt ggatgcgtat
    43081 aaggcgagcg ccgctgggaa tctcctcaat agggagctcg cggctggcga aatcggcggg
    43141 cggaagtgga ccgggactag gcggcgccat gttcgccgta ggccgccgct gccgtcacca
    43201 cgtcttcgat tttgcccttg cgaagctgat cgatcggcct cttgccgcca aggcgctgat
    43261 cgcccgtgag catgaagttg acgcgcatcc atgggtcgtc aactgtgaag gcatcgagca
    43321 cctcccggat gccgggcagc aggccgttct tgccgatctg gaaggccggg tagacataac
    43381 cgtctccaac gtcgagccag aagacctggt tacgctcccg cttccggccg aggccctgcg
    43441 cggtaattcc tagatgctcg gcgagctgct gcgcagagag tgcaccgcct tccgccttca
    43501 gcatttcgcg cttcatctct gatccgcgga ggaaagcctc tgtaagcgga tcgggcttgt
    43561 ccggctcgat ttccgccccg atggcggcgg cttcacgcag tgagctaaac agcacatctg
    43621 tgtcggtcga cgcgccgacg gcggccaaca agcgctcgcg tggcatgcgc tcggcaatgc
    43681 gtgcgagagc tctgccggtt cgaaggacaa aggcctcgac aagatcctcg ctcggagccg
    43741 gctccgagcg gcggctgcgc gtagctcccg tggacgtggc gtttcctccg aagtggctgg
    43801 cgcgactgcg gcggcgagtt ccggctgaag ctgtgggcgc catggcgata tcccttggaa
    43861 aggttcaatt gtcatgatag atatagtgcg tgaaaacgga aaatgcaatt gcgattgtca
    43921 tgcaagcaca gaatcccgcc aattgtgcga ttttgcactc ctccagggtt cgagaccctg
    43981 cgccaagagc gccgagtggc gccgggtact ggaaattgtg cgccggtaga atgcgccgca
    44041 acccattgat gtgcgtgctc ttttctcgat ttcgtgcaat cttgagtgaa tggatgtcaa
    44101 ggctgcagaa cccgcttcag cggaagtcat cgacttcttc gactacgcct cacccgagcc
    44161 acgcacaagc cctcggagga gttgcattca gcgccgaccc gaggccactc gatccgggat
    44221 ggagatcact gacgattggc ccgcacgcgt accagttacc gaggctgaac tggacctgtt
    44281 cgaagcccat ttcggcccgc tcattgatga gctgctgcgc tcgacagact gacttgctga
    44341 acggaggtcc tgcatgaacg ccagagcaac cccagccctg gtgccgccgg ggcgtgccgc
    44401 tctctatctc cgcgtctcaa caggccgcca agccgagcat gatttgtcga ttcccgatca
    44461 gcggcggcag cttcacgctt attgcgacgg gaaggggatt gccgtggtcg ccgagttcgt
    44521 cgaaccggga gcatccgcta cggacgaccg tcgtcccgag tttcagagga tgatggacgc
    44581 cgcagcggag aagcccgcgc cgttcgactg cattatcgtc cactcattct cacgattttt
    44641 ccgcgatcag ttccagctcg agttctacgt tcggcggctg gcgaagaacg ggatacgcct
    44701 gatttccatc acgcaggatc tcggtgatga tccaatgagc gtgatgatgc gtcagatcat
    44761 ggcgctgttc gatgaatatc agtcgaagga gaatgccaag catacccttc gcgccatgcg
    44821 agagaacgcg cgccagggat actggaacgg ctcgcgcccg ccgtttggct acaaaatcgc
    44881 agtcgctgag cagcgaggcg cgcgcgcaaa gaagaagatt gagccagacc cctcgcagac
    44941 tgaaactgtt cggctcatgt tccgcctcgc tagaatcggc agtgaagacg gtcccatggg
    45001 ttcgcgtcag atcgccgact accttaacgc acatggcatt cggacccaga cgggtggccg
    45061 atggggcgtc ggcgcagtcc accagattct tacgcgtgaa acatacattg gcaggcatcg
    45121 attcaacgtc tcgggcaagc ggaagggcga gaggaaatcc gaggatgaga tcgttgaaat
    45181 ggcggtcgag ccgatcatca gcgaggaaga gttcgccgaa gttcagaaag tgatgcgatc
    45241 gcgaagcccg cagctcagag cgcctcggtt tgtctccgct ggtacgctgc ttggcggagt
    45301 gtgcttctgt gccgactgcg gcggagcgat gacgcttcgc acctccggaa agggcgacca
    45361 atatcgttac tacacctgct gcaccgctgc ccgacagggg aagagtggct gccgaggacg
    45421 caccattccg atgaatgagc tggacgagat ggttgtgggc catctcgagg agcggctgtt
    45481 ggcgcccgat cggctggagg aactgctctc cggtctcctg aaacgccggg aggagcatac
    45541 ggagcgtcta cgagggcggg tcgccgaact gaggaagcag gcggcagacg ccgaagccaa
    45601 gctcacgcgg ctttatgagg caatcgagaa cggtctcgct gacctagacg actgcaacct
    45661 gaagggacga attgtcgagc tgaagcggat gcgcgatgct gccagggcag acgcggaccg
    45721 ggcggaaagc cgtgcggacg ataagggcaa acggctgacc cccgagcttt tgcgccgatt
    45781 tgggatcgaa gcgcgcaaga agatccgggc gcgtgacggt ggtttccgtc gaagccacgt
    45841 ccaggcgctg gtccagcgta tcgaggtggg aacagacgaa attaggatta tgggttcgaa
    45901 gccaaggctc ctccagacgc tcgtgacgtc cggaggaggc tatggagtgg aaacggcgac
    45961 gcacggcgtt cgcagtttcg ttccgaaatg gctccccggg ccggattcga accagcgacc
    46021 aaccggttaa cagccggttg c
//
LOCUS       DAORNH010000005        45447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_607_length_45447_cov_2.737949, whole genome shotgun sequence.
ACCESSION   DAORNH010000005 DAORNH010000000
VERSION     DAORNH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 45447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45447
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_607_length_45447_cov_2.737949"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK264_00905"
     CDS             <1..189
                     /locus_tag="PK264_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HRD74489.1"
                     /translation="KRAAPKAGIVSKAKDAAKNIGDRTKATVKDAYGRAKDAIAPAPT
                     TPAPAPATPAPAAPAPKQ"
     gene            337..2226
                     /locus_tag="PK264_00910"
     CDS             337..2226
                     /locus_tag="PK264_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRD74490.1"
                     /translation="MDLLFRIIYAAHARGTHHKLALDGLRYLKGPTAERWQRVLMKHA
                     KDLMAGAKAPDDEFKDFKNHVLHVRDNFWGGAPEKARSWYDHLVDVLESRDWRSAAWC
                     AGILSHYVTDPIHPFHTGQSEAENNVHRAVEWSINRSYDALRKQADAEFAGEMVVLGE
                     GADWLERAICDGATRANAYYEKLIAHYDFTRGVVEPTAGIDQVGNRISAELIAYAAEL
                     FGKVLDRAIVEARVAPPDVTLTLETVLAAIEMPIKALQKKLADAGDRRQVERMYDELV
                     ATGTVETNLPDDDRMVRDLHRAEVLAKLPTASIEGRFTPPVREKIETMVARRRRAERE
                     TVAASLAQTVPSASTTVAMAAATSDAAVPAPASPATPPAPRAMDRRQSQIGGSSELGD
                     ADVEIRKSLRGDAGAPSAPPPVPGSDGRPAALPATGASLLARLGDVGSAVTGSAAVAP
                     AVRVTASPKTAAFHQLLEPDGAAPVDASIREEREPRAGRPRIARDSAVVDAPSIGPKT
                     ADRLITAGIRTIGELIDAEPEALAARLATRHITPAILRDWQDQTRLVLAVPVLRGTHA
                     QLLVGAGLRTAEALRDAEPASLCSAVLAYAANADGQRILRQGDPPDIERIKGWVDAAR
                     TALAA"
     gene            2448..2984
                     /locus_tag="PK264_00915"
                     /pseudo
     CDS             2448..2984
                     /locus_tag="PK264_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            2884..3993
                     /locus_tag="PK264_00920"
     CDS             2884..3993
                     /locus_tag="PK264_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD74491.1"
                     /translation="MVPAAPCDRCSGCASRDRTTRASTSSPTSAWRTISRPSGAPSSS
                     RAPIRAAPCSFTDDLWRLDYQLRDGEDEAEAVREETIRARIAAILGDIGHAGPWELEW
                     WSIYTANTLLLDDYRAGRVFFIGDSAHIVPIFGVRGLNNGLADAFNIGWKLAYVLAGK
                     AGPGLLGSYTPERRGATLDVFANAGKSTRFMTPPSRGWRLAREAALSLAIRHAFTRPL
                     ANPRQMQAYTYAESPLTPFPARDQEFAGGPRSGSLAPDVRLQDGTHFRSRIGDELTIL
                     LFADAASLPRHKAQVRASPGAAELAVVTVVLGPDCADAAAILDTDHRIARAYDALDGT
                     VYLLRPDHHIAARWRQLDRCELEAAIAACLGKETP"
     gene            3990..4190
                     /locus_tag="PK264_00925"
     CDS             3990..4190
                     /locus_tag="PK264_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74492.1"
                     /translation="MSAEQDHLEAAYEALAAAIDTAGGENEALFLTKLALILADRIAD
                     PAAFEAAIAIALEDLPGAAERH"
     gene            4275..5165
                     /locus_tag="PK264_00930"
     CDS             4275..5165
                     /locus_tag="PK264_00930"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD74493.1"
                     /translation="MSKVAWLGLGVMGYPMAGHLVKKGGHDLVVYNRNGAKSAAWAKE
                     FGGRTAATPAEAAKDADFVFSCVGNDDDVRAVTIGKDGAFASVRKGAIFIDNTTASAN
                     VARELYAAAKAKGFDFLDAPVSGGQAGAQNGVLTVMVGGDEAPFERAKPVMAAFARAV
                     TLIGTSGAGQLTKMVNQICIAGLVQGLAEGVHMAQKAGLDLAKVMDTISKGAAGSWQM
                     ENRWKTMAEGKYDFGFAVDWMRKDLSISLDEARRNGATLPVAALVDQFYGEVQRLGGN
                     RWDTSSLLARLNNQKNDKKA"
     gene            5162..5608
                     /locus_tag="PK264_00935"
     CDS             5162..5608
                     /locus_tag="PK264_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HRD74494.1"
                     /translation="MSQTSPGPMRRPSPMEVLTYMGSINPATQHLDIKVLAAEPGRAR
                     FAMLVRPDMANTFGTCHGGIIFTLADTAFGWTGNATNEKAVTAGASIEILAPAMVGDR
                     LIADAVEVFREGRNVLLDVRVWREGTDVTVAHVRGRMRQVGGALLT"
     gene            5670..6815
                     /locus_tag="PK264_00940"
     CDS             5670..6815
                     /locus_tag="PK264_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRD74495.1"
                     /translation="MTAERNEPRIFRGLKDVYFERSDCTRIDGKAGELSYRGYSIHDL
                     AAHSTFEETAYLLLNGALPKTYELAAFDAGLKAERQLPAPILDIIGAVRAGHPMDVLR
                     TAVSALAAFDPQVTDNGRAATIAKAIRLTSQVPMIVAAHARLRVGKPVIAPEPALGHA
                     ANFLWMLKGVQPSEDAAKLLDTDFVLHAEHGSNASSFAARVVIGTDANIHAAMTAAIA
                     TLSGPAHGGAAEDVMQMATEIGDPENVAAYVKGKRAAKEAITGFGHRVYRVEDPRARH
                     MREGVKRLSLEMGEPRWYQILQGVVDEMKPYARHGVNVNVDFYSGVIYHLHGIPKDLF
                     VPIFAVGRVPGWAVQVLEQQANNILIRPLTLYSGPEARPYVPIAERA"
     gene            complement(6878..7540)
                     /locus_tag="PK264_00945"
     CDS             complement(6878..7540)
                     /locus_tag="PK264_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74496.1"
                     /translation="MSYRFFAAVAAFAAVATVAGSALTSSAEARSGTGHVPYGVNGAD
                     PDPHPASNGPPVSRAPATSFRGIRNFSLIDGEPIGFGNFNKRADASYRTGERVVVYGE
                     PVGFGWSARGSGYHFQLFASIEIRDRGGRVVWTAPRRVNLENSAERQTSDFYFHVFLE
                     LPNLSPGAYVVHYKFDDPQARTFVNFERNITVTSDRTTDAPAPGAPPVANDHRMPVRE
                     SH"
     gene            complement(7798..10635)
                     /gene="tolA"
                     /locus_tag="PK264_00950"
     CDS             complement(7798..10635)
                     /gene="tolA"
                     /locus_tag="PK264_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HRD74497.1"
                     /translation="MSSVAKKSGSVADDVVSSMTMVDDYKGSVVFGGETAGYQNAVGM
                     YTIGADGSISNVRIVFSNASLKGSGGDLLAGKSAVDVSLEAGAKVGFFVVPNGFSQPG
                     MAKVLADTAGSFKFVNADGSAGNINSGAEMKLVHTSASGAETTVKSAYGTSTFHSVDT
                     GSMGLNGDKLDHVKTTVDPDAGTVRIAFEDLKGGGDNDFDDSIIVVNVGASNAAALKA
                     GTAEAIVAERAAAAEALTAESASSGPTEMLIDGSFEQGIVGANSWSHQKTVGGWKSDS
                     EIEVWGKGFYGLKAADGDKIAELDYNTKASNIFQDVSTEAGTEYTFSFDFMKRPDSKA
                     GSDTIHVFWNGELVGTVEPSKSEWTKAAFKVVGTGGSDRIEFREDSADNDSYGGLIDR
                     ASLMRSGPTAAERAHQAAKAAEEKAKAEAEEKARLEAEAKAKAEAEEKARLEAEAKAK
                     AEAEEKARLEAEAKAKAEAEEKARLEAEKNKDALLLKTSDVHDVKTGEHLEGNRDKNA
                     IDGGAGEDEMFGRSGDDVLNGDGVARVTIPLDIAVRLVDADGSERLSILVDNMPEGAV
                     LSAGKDNGDGSWTLSSDDLVGLTLTGPDSANFSLKVVATTHGASNLTEASEINVSLVR
                     GNNDLMMGNGGNDKLDGGAGDDVMYGGTKPTGEVKPHASTVADNDVLHGGAGNDVMYG
                     NAGDDEMFGEADNDTMSGGKGNDLVDGGDGDDVLTGNSGDDVVIGGAGNDVMKGNAGN
                     DTLVDGEGDDVVEGSAGDDVVRAGAGNDSYNGGSGFDTLDFSAADRGMMIDLSKKSIV
                     GMGEDKVANFEVVVGSSHDDVMKGSKAAETLDGGAGDDDLRGLGGADVLTGGEGSDTF
                     RWSLTDVMADGKHLGVDRVTDFSKEDVLDLSRTVDGSFASVDDAVLVKDDGSASHVYA
                     RVAGEWVEVVVLEGVSGMTAGDMHKSGMLVL"
     gene            complement(10955..11575)
                     /locus_tag="PK264_00955"
     CDS             complement(10955..11575)
                     /locus_tag="PK264_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74498.1"
                     /translation="MTTGFRKTILAAALSISAFALAATTATAQSNGARPIPSNGFQAA
                     PGSGDIVPPPAAPTMLPNHLTGPSGLVNIAMTERKAEGFRKFLVRTVRPYLPGETIHL
                     YAEPMNFGWSQIAMGYRFHLSTVIEIHDANGRILHRLPIAKDITGQTTDPRNIHITFG
                     FKLPENARPGIYTITFIFTDPVNNRTVSVKRPFAVDSGRHAGMIDG"
     gene            11779..15000
                     /gene="ileS"
                     /locus_tag="PK264_00960"
     CDS             11779..15000
                     /gene="ileS"
                     /locus_tag="PK264_00960"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRD74499.1"
                     /translation="MAKTPKQETATTADGRDWGATLFLPKTEFPMRAGLPLLEPKIIE
                     RWQQIGLYEKLRAKAKGRPRFILHDGPPYANGNIHIGHALNKILKDLVTRSQQMLGFD
                     SNYVPGWDCHGLPIEWKIEEEYMAKGKAKPNLKDPAAMTAFRKECRAFAENWMRVQSE
                     EFQRLGVVGDWKNPYSTMTFPAEAQIARELMKFAETGQLYRGSKPVMWSVVEKTALAE
                     AEVEYQDYQSDMVWVKFPVKAAAAVTQHRDAGSTSPIAAQVLTGLAAEATRGPVSIII
                     WTTTPWTLPGNRAISYHGKIAYGLYEVTKAPDGNWARVGERYVIADKLAAEVMKAAKV
                     EAFERRGVVSADDLSRTTCAHPLAKSGLGGYQFAVPLLDGDHVTDDAGTGFVHTAPGH
                     GREDFDIWTASTRALQARGIDPAIPYTVDENGAFTTDAPGFTGERVITDKGEKGKAND
                     AVIKALAAAGMMVARGRLKHQYPHSWRSKKPVIFRNTPQWFIHMDKEVPFLAEAAKAH
                     RAKRAAAGMAEGHNQPTPKTLREVSLKSIRETQWVPASGENRIAGMIEAKPDWVISRQ
                     RAWGVPITVFVRKATGEVLFDPKVNKRIYDAMFKEGADAWFADLDGARFLKPDHDPND
                     FEKVNDVLDVWFDSGSTHTYVLNDPHFPGLMGITRKVDGGRDTVMYLEGSDQHRGWFH
                     SSLIESSGTRARAPFDVVLTHGFTMDEKGQKMSKSVGNVVAPQDVMKTYGADILRLWV
                     AASDYADDQRLGPDILKGFSETYRKIRNTFRWMLGTLSVVASGTSAGSRPEATAGEAG
                     KMPELERLMLHRLAELAPIVRKAYAEYDYAKVVTSLSQFMNTELSAFYFDIRKDALYC
                     EAPSSPKRLAALACVEEIFRATCIWLAPILSFTTEEAWLARYGDAMKEGSVHLELFPD
                     SLDGWLDEALARKWEKVRRVRSAVTASLEHERAAKRIGSSLEAAVTVAIEEAGVAEAL
                     TAEQWAEVFITSRADVRTGKEDMLALNVASGSATGKAHYRADVEPNHGVAGVVVQVAA
                     FPQPGWKKCARSWRFTQDVGSDAAYPELSARDAAAMREIDKRA"
     gene            complement(15027..15809)
                     /locus_tag="PK264_00965"
     CDS             complement(15027..15809)
                     /locus_tag="PK264_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74500.1"
                     /translation="MMRLGGGERERLRSDDGIGRPAPRTRNCHKVRHLASRLGETFAG
                     HRMRTTVVEQTCALELVEGHVDEVWAWPWRSASLDELPPNPAGRFGAWEVRCGPLGRR
                     QRCALLHVAGPAVDVAGAEPRISTHFIMDAVAGRESVVWRVIVGARGATPAAAPKRDL
                     GDEPGARPATSAGDIGITFALGSGLVRVAYAMCRSGTCVMEAGPRHASEIASRLADGR
                     PVEIRLAGDQDEGQAIVVPPAGFRAGFNALAKLRREELRGPR"
     gene            complement(16055..17176)
                     /locus_tag="PK264_00970"
     CDS             complement(16055..17176)
                     /locus_tag="PK264_00970"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HRD74501.1"
                     /translation="MSEPASCSPSRPLPPGSRIGILGGGQLGRMLAMAAARLGLKTHV
                     YSDESGPAFDVATEGTKARYDDTAALDAFARSVDAITYEFENVPVETAERLARTCPVR
                     PGAAALRVAQDRLVEKSTIQSLGIPVAPFHAVDSLADLERAMVAEPAGAILKTRRLGY
                     DGKGQVVLAAGVRAADALAEIRNAPAILERRLSFAFEVSVLIVRGLDGATAIYDLPRN
                     EHGGGILRRSSVPSLLPAHVVARAQDIARRIATHLDYVGLLAVELFYMGPDAPEPLVV
                     NEIAPRVHNSGHWTIEACGVSQFENHMRAVAGWPLGSPGRHSDALMVNLIGPDANAWS
                     ALAADPGVCLHLYGKRESRAGRKMGHWTKLSPLTHEPAR"
     gene            complement(17169..17666)
                     /gene="purE"
                     /locus_tag="PK264_00975"
     CDS             complement(17169..17666)
                     /gene="purE"
                     /locus_tag="PK264_00975"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRD74502.1"
                     /translation="MTAPQPQVGIIMGSQSDFATMSRAADILTTLGVPHEVRIVSAHR
                     TPERLYDYARSARDRGLKVIIAGAGGAAHLPGMTAAMTTLPVLGVPVQSKALSGQDSL
                     LSIVQMPAGVPVGTLAIGEAGATNAGLLAAQILAITDAALAARIEAWRQAQTASVAEA
                     PKPNV"
     gene            complement(17757..>17939)
                     /locus_tag="PK264_00980"
     CDS             complement(17757..>17939)
                     /locus_tag="PK264_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74503.1"
                     /translation="RTTFIIAHRLSTVSDADEILVLDKGRIVERGTFQSLVAEKGLFA
                     RLVAEGGFTVPVQPDP"
     assembly_gap    17940..17980
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17981..19510)
                     /locus_tag="PK264_00985"
     CDS             complement(<17981..19510)
                     /locus_tag="PK264_00985"
                     /EC_number="7.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan export ABC transporter ATP-binding
                     protein"
                     /protein_id="HRD74504.1"
                     /translation="MTTPSLTTPQVYKRAVSLLASERGLAVALALAGIAVAVVQLAEP
                     ILFGRVVDALSKGQEAFPIIGIWAGLGLFGIFASVVVAVASDRLAHRRRLAAMSGAFE
                     RAITLPISYHAEKGSGAVVRTILSGTDALFWLWLGFLREQLTAIVGILLLVPTAIRMD
                     ARMAAILAALAVAYLVLNLFVVRKTSDGQAAVERYHNDVYGRVGDVLGNVTVVQSYAR
                     FSAEMAAMQGLMRDLLAAQYPVLTWWGLLTVLTRAAATITMVAVFSVGAVLAQKGEIS
                     VGQIVSFVAFAGLLIGKLDQLSGFVIRIYQQAPTLRSYFDLLDQSAAIVEKPGAKPIG
                     AVQGQVAFERVSFRYGTGAQGVFDLTFEAAPGKTVALVGPTGSGKTTTLALLQRLRGP
                     ESGRILVDGHDISDVTLVSLRQSMATVFQDAGLFNRSIAENIRIGRPEASDAEVERAA
                     KLAEAHEFILAKPDGYKFVIGERGASLSGGERQRIAIARAILKDAPILILDEATSALD
                     TE"
     gene            complement(19552..20130)
                     /locus_tag="PK264_00990"
     CDS             complement(19552..20130)
                     /locus_tag="PK264_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74505.1"
                     /translation="MTQSAPKLNYWNATWSLDEKQCPCDIHFLDYLEATGMRGKAIFH
                     MGTGNHHIIGLKTASNGSGNHVLGITASREEYDDYVKLLIANPRLGFTYKAYFGDLYQ
                     IDGKLLPELDLVTLFHLGEYRTEANDSYGALTDLEATLVLADRLRAGGEIHFYSGSFA
                     YDKAAAAARELARLRSFEEAEPFKTLRIWRKR"
     gene            complement(20160..>21459)
                     /locus_tag="PK264_00995"
     CDS             complement(20160..>21459)
                     /locus_tag="PK264_00995"
                     /inference="COORDINATES: protein motif:HMM:NF017775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74506.1"
                     /translation="DDSRWATEPLTDIAIGLLQATVGFALFVGVLWTVGGSYEIGGVT
                     IPAYMVLLALGYGITASVLMAWIGRPLVGTVGNKNAAEGYFRFALTRTRENAESISLL
                     KGAETERAIIGGIYRTVVERWLRIVRQHANITWITNSVGPMTPVVPLLFAVPKYFSGE
                     LTLGQTTQLAGAFVEVQAAISWVVNNYNRIAEWYASARRVMDIVDAADGVDAAAAASP
                     AISVAAAPASLLALTQLRLDDPNGRPLIASASLTAKPGAITHVAGGSSLGKTTLVKAI
                     TGLWPFGAGRIERPAGEIMVVPQRAYVPLGSLAEALCYPDIRAERAAIEAALDQVGLG
                     TLKARLDENARWDQELSIGDGQRLAIARVLVHKPAALLLDDAFSALDADTEALLLGLV
                     RECCPGAIVVTLDQRPAREPEGVAAHILHRAAGGAVLKPA"
     assembly_gap    21460..21475
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21476..21895)
                     /locus_tag="PK264_01000"
     CDS             complement(<21476..21895)
                     /locus_tag="PK264_01000"
                     /inference="COORDINATES: protein motif:HMM:NF018210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74507.1"
                     /translation="MTAAADATPETTGFHLDRRVTKAFLAYSGGWWRGPTARQAWLLT
                     ISLAACLILGVLAQAALNWWNRWFFDALGRKDAASAMQSVPVFFAIVASMAAIGVGIV
                     IARETLQVRWRAWLVERLVGSWLGGHRFYHMGLRRTEP"
     gene            complement(21927..24308)
                     /locus_tag="PK264_01005"
     CDS             complement(21927..24308)
                     /locus_tag="PK264_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74508.1"
                     /translation="MASSNRPPRRAFNPERERWSLADADGYAIALAALAVLWIVLVWP
                     WARGEVTIPWDAKAHFQPQIQFLAQSLWSGEWPSWTPYVFSGHPQIADPQSMIFSAPF
                     LILALVNPSPSLWAVDMTVLLSGLAGAVALALWFRDQEWHWAGALIAGLVFCFGASMS
                     WRMQHTGQVLSLAALPFALLWLDRAIDRGSFIYGMLAGIAAAHMLLGRDQVALICLYI
                     LAAYVPWRWLSDDEPRATILRSILPLMLGGLIAVALVAVPLLLTASLAAESNRPAIDF
                     EGAGRGSLHPALLVTFLAPQIFGAAGAMADYWGPPSFTWHDTGLYIAQNMGQVYVALI
                     PLLMIALGLFSGRLWSREIRFFTVALALVILYALGWYTPVFRGFYEVLPGVKFYRRPA
                     DATFVIGALAAILSGYAVARMFEAPWLRPRGLHVLGVAALVVAGLILMLAIGWRLDRL
                     ERAWTPLLIAGGWLLLAVALIAWAWPRVALSPHVAALALAVVTTADLAWNNGKTTSSA
                     MPPEMYEAMEPTTRNETITWLKRLTGEGKSDTRRDRVELVGLGFHWPNLSLTHRLEAT
                     LGYNPVRLGRYSRATGAEDTVGLPDQRKFAPLFKGYASPLARLLGLRYIATPIPIEKI
                     DKAAMPGELTLVVGTAEGYIYENRAALPRVLFAIEARPLGDPEAIITSGRWPDFDPRT
                     TVLLEGPLPASRRRRPGTVRILDYRNTDVVIEATSSDGGFLVLNDLWHPWWQAEIEVQ
                     GGALLPAPILRANLLFRAVEVPAGDVKVRFTFNPLAGLLQSLRQRSASTPASH"
     gene            25084..26265
                     /locus_tag="PK264_01010"
     CDS             25084..26265
                     /locus_tag="PK264_01010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD74509.1"
                     /translation="MDFTIPKELAELRDKVAAFVRSKIIPFEKDPRWGHHGIPEDLRR
                     DLNATAKEAGLLGINSPEEFGGKGFSHFEQAVVFEAAGYSILGPIALHFHAPDEGNIH
                     LLDVVATPAQRKKYLAKLATGEVRSCFAMTEPDGGAGADPLLLKTRATPDGNGFVING
                     RKTLITGAEGAAFTIIMARTGDGPGDCTMFLAELPSKAFKIHRVLNSMDSSFMGGHAV
                     IDIENLRVSGDDILGEVGKGFQYAQVRLAPARLTHCMRWLGSANRAHDIATEYARHRK
                     SFGKTLGEHQGVSFMLADNAMDIHTARLAIWHCAWLLDQGDKASTMSSMAKVICSEAI
                     FRVVDRSMQILGGSGVTDDTMVERIFRDVRAFRIYDGPSEVHRHSLGRRIVQGDRKKH
                     A"
     gene            complement(26281..27171)
                     /locus_tag="PK264_01015"
     CDS             complement(26281..27171)
                     /locus_tag="PK264_01015"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD74510.1"
                     /translation="MAISIRQIHPVFVGEVSGADLTKSLSADEVAAIEAGMDRYAVLV
                     FRGQAISDDQQMDFTLNFGRIENREGGNITKAGEYRLKTGMIDVSNLDQSGKPFERND
                     RRRMFNLGNRLWHSDSSFRATPAKYSLLSGRIVADHGGDTEFADMRVAYETLDDATRA
                     TCDDLVSEHSLIYSRGSMGFTELSEDERAMFKPVRQRLVRTHPVSGRKSLYLSSHIGG
                     IVGWPMPEARDFIRDLAEHATQRAYVYAHKWRQFDLVMWDNRTTMHRVRRFDETKPRD
                     MRRTTVAGDGPTVTEQVAAE"
     gene            complement(27270..28085)
                     /locus_tag="PK264_01020"
     CDS             complement(27270..28085)
                     /locus_tag="PK264_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD74511.1"
                     /translation="MSEPSTPEQEAVPARPSATIMLVRDGRDGIEIYMVMRDRPMDGA
                     MGAVVFPGGKVDAEDGPEAWSGATLEPPQSHPDLPFWLAAMRETFEEAGIVIARPIGT
                     RTGDVAPSEARRMVGAHREALLDRKLTFAEIIRQERLAPALDHMVHFAHWQTPFGLPK
                     RFDTHFFLVAAPEGQEPMHDGREMVDGFWTRPEAILDEARRKVRTLVVPTRLNLELLA
                     ESRSVHEAMERARARRIVCVRPERTQSANGEWVVAIPADAGYRTAILSSSRQK"
     gene            complement(28164..28967)
                     /locus_tag="PK264_01025"
     CDS             complement(28164..28967)
                     /locus_tag="PK264_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD74512.1"
                     /translation="MRTPRGLHVEIREPEVAATTPRRPIVFHHGIGTNHRIWDGWLPA
                     IGPDRGVARFDLRGFGRSDVPDVTHKWTMQELVDDLFEVVDLAAGATGRAHLVGESLG
                     GTVVLLAAILRPDRIASVTISNAAFKGTGIHRVAGWREEIARDGMAGWAARMMELRFR
                     DGVLAPEKAKWFADVQAASPAHVVLGLGELLAVQDLSTQLAAIKAPLLILMPTESPFV
                     PVEQARELGTRVQGASLVLFHGVRHGLPYSHAEACARHLAGFLQRIDPA"
     gene            29068..30117
                     /locus_tag="PK264_01030"
     CDS             29068..30117
                     /locus_tag="PK264_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD74513.1"
                     /translation="MKFGYFTLSDNHYPGLGRSPEQFILEIREQAIHADRIGMNSAWI
                     GEHHFDTLGVNSCPHMLLASIVPVTKRIRLAPAVAVLPLHHPLKVAEEWATLDLISGG
                     RVDFAVGRGYDRREYVPFGADYMSSLEILDESVEVIRKAWTSPGPWSHKGRFFDIPEM
                     QITPRPLQKPIPFYMACFSPSSMEIAARRGLDIIFAPFAAQMTFGGLAEAVAAYRAAC
                     AKAGTRPGRAMCSYFIHIADTPAEDDFGRESLMRYFEHAVRPAFKQEPGKTPPTMAYF
                     QKINEVLAGMRKESLSERSILLGPARRITEVLKSVEAAGISEVILYFNVGGKPHDLVL
                     GQMDKFMKEIAPAFS"
     gene            30209..30285
                     /locus_tag="PK264_01035"
     tRNA            30209..30285
                     /locus_tag="PK264_01035"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30243..30245,aa:Arg,seq:cct)
     gene            30370..31554
                     /locus_tag="PK264_01040"
     CDS             30370..31554
                     /locus_tag="PK264_01040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD74514.1"
                     /translation="MPFEPASDVLSSYTVLDLTRVRSGPTCVRQLADWGANVIKIEDP
                     ESDKVGLGGPRSGSDFQNLHRNKRSITLNLKSPEGAEIFRKLAAKADIIVENYRPDVK
                     TRLGIDYAAIAKLNPRIIYASISGFGQTGPLAKRPGFDQIAQGMGGLMSITGEPGKGP
                     MRVGIPIADLTAGIFCAQGILLAIIERERSGKGQWVQTSLLQAQVFMLDFQASRWLMD
                     QDVPGQAGNDHPTSIPTGVFPTSDGHINIAVAGQAMWQKFKAVLADPALEHADYADGK
                     SRSKNRKALNALIAAHTVKASSAEWIERLNKAGCPSGEINTIDKVFGLEQVRALGLAQ
                     PMVSQERGKTEVLGQPILMSRSKASVKVPPPALGQHTAEVLGEIGYTSADVTKLKKSG
                     IV"
     gene            31609..32409
                     /locus_tag="PK264_01045"
     CDS             31609..32409
                     /locus_tag="PK264_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD74515.1"
                     /translation="MAEAPAEEKLLSRRDGAVAHIIFNNPARHNAVSLEMWQRMHMLL
                     DELAADPGVRMLVVSGAGGKAFVSGADISKFESERASEEAVKLYNATSAGAYEKLYHF
                     PKPTIAKIQGYCVGGGMNLAVCSDLRIANESAKFAIPAAKLGLGYGLPGVRRLSEIVG
                     ISRTMELFYTAKQLSAREAHSIGLVNEVVPDGEIDAAVERLTATIAGNAPLTIATIKA
                     CARELGKPESQRDLAKLDRMVQACFESRDYTEGRRAFMEKRKPVFEGR"
     gene            32568..33443
                     /locus_tag="PK264_01050"
     CDS             32568..33443
                     /locus_tag="PK264_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD74516.1"
                     /translation="MRNSIAVMNTKGGVGKSTIVLALAETLSAFHGKNVLVIDSDSQA
                     SVSAMLMSVSNLRRLQGEGRTMVDYLIATVLNNEPVEWPNYVVSGVSDVDDARTVYLM
                     PSDMQLTLFEREVSKESLHSRLRTSINGLLARARIVFDVILIDCPPGLSVLTESWLRE
                     ADFHVSPTKPDYISTCGLDVFNRFRGLNPEMGFATNLGVLVNMKDPMSQADEEYQRWL
                     QQNPENKCFTAVLPRVNALQHTVRFRDQDRSFMAKYPGDAGAAVRALANELLTRIATE
                     QAPANLTPKAATSVA"
     gene            33577..>34418
                     /gene="dctP"
                     /locus_tag="PK264_01055"
     CDS             33577..>34418
                     /gene="dctP"
                     /locus_tag="PK264_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRD74517.1"
                     /translation="MKSSKIREAKRLALSGAATAAVALGFAMSSPATAQVVDGPVVNW
                     NHSVWGKARAYTAGMETVVAYLKEKTGGKFTIKIHYGEALSKDKENLDGIKIGAFESA
                     DFCNFYHPGKNPAWMVFTLPFLPIGDWDVSRDVRMKMLEHPAFVKDMDQWGAMAYISG
                     LLPQYEFLGKGTPPKTLDDWKGRRVRAGGGVGDAMRLLGAVLSTVPAAEVYTAIERGT
                     VDAASFPYTYAHASYQIHTVTDWFTSNMSPGTSECAAVFSKEAFAKLPDAYKKLLMDA
                     RAPA"
     assembly_gap    34419..34440
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34441..34661
                     /locus_tag="PK264_01060"
     CDS             <34441..34661
                     /locus_tag="PK264_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74518.1"
                     /translation="QDVDKVNLPLFRARLKEVVYTPEELKVFQDKVGRPVWDKWVADN
                     KAKFDAKKVLDDLLAAIAAAEAKYKKKT"
     gene            34773..35489
                     /locus_tag="PK264_01065"
     CDS             34773..35489
                     /locus_tag="PK264_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD74519.1"
                     /translation="MAHAKAVDPTATTGGGLLGAFDRGLHWIEKGLALASGFGIFALM
                     MIGVVQILSRKFLNLPIFGYIDMVEIAMAFLVFLGLSYTERLGGHIRMELIVAKLKGR
                     ALWLFEIIGVTCGLIIVGVLTVFSWQHAMRAYNSGDSSIDAQFQLWPSKMVVSISLGL
                     LFLRLLVSLWAYIRLLISPDAAQVAVIEVVDSEEQARREAEAALQATLGDDPGLAAPA
                     AAGPATLNSSRTNASSGPQS"
     gene            35486..36796
                     /locus_tag="PK264_01070"
     CDS             35486..36796
                     /locus_tag="PK264_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD74520.1"
                     /translation="MTEFSKDAIGIIGCLALLVLVFAGVRVYLAAAVVGFLGMAAMLG
                     FYRSAAILGTIPHSKSVSYTLSVLPLFILIGYLAYHAGMTQALFRAAKAWIGWLPGGL
                     AIASIIAVAAFSAVSGASTAAAAVFARVAIPEMLQAGYDKRLAAGVVAAGGTLDALIP
                     PSALMVVYAIIVEASIGKLLVAGFIPGFISAACYGLIVIVWSWWNPSVGTRLTGYTWR
                     ERFVSLPGTFPVVFVVVIIFASMYYGWATPTEAGALGAFVVFLAALLNRVKFAQVNDA
                     LLETAKLTVMIFAIIWGILIFVRFLGYCGVPERFSEWVVALPFPRWVIMLGILGVYLI
                     LGCFMDAIGMLLLTIPVLYPAVIKLGYDPIWFGIIVVKMCGVCLLTPPIGLNCFVVSA
                     VRPDIPLSTVFKGALPFVIADFIAIGLFMIWPEIITWLPDQVIK"
     gene            36813..38132
                     /locus_tag="PK264_01075"
     CDS             36813..38132
                     /locus_tag="PK264_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD74521.1"
                     /translation="MSDPGQPRRGAAELPPAQSSTDAGGILVAVIVLTFLMNTIGRGL
                     TETFAVFLLPVQTGLNATRAEISATYSVYMLAYAFAAPFAGQLIDRLGARVTYGLGLS
                     ALGLGYALGGLSTEVWHYYLAIGLCGGLGAAALGMVAASSLLSRWFSRRMGSVMSAPY
                     AAVGAGMLIFPPVTQLLIDWLGWRSALYVLGLGTLAILPLTLALPLGRMTAGSSEWRA
                     ARDQAQRAAGGWTLTAASRTGAFWGLFAAYFWTSVAAYAVLPQSVAFLVEQRFDPLVA
                     AAAFGVTGSLSVCGILVVGWLADRIGRLPTVTMTYLMSIAGTLALIGVAVWPSLALVY
                     TFVVFFGLVQGARGPIILSLLPTLYPGGAVGAIFGALSMALGLGAGLGSLASGYLRQL
                     TGGYTASFALAALASALGLLTFWIVPSLRHERLGGKGPPPQTTRPLT"
     gene            complement(38139..39143)
                     /gene="pip"
                     /locus_tag="PK264_01080"
     CDS             complement(38139..39143)
                     /gene="pip"
                     /locus_tag="PK264_01080"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRD74522.1"
                     /translation="MREGTTTSDRRRRGLYPPIEPYRQDRLAVGDGHVLYYEESGNRH
                     GKPVLVVHGGPGGGSNPTMRRYHDPSRYRIVLFDQRGCGRSTPNACLEANTTWHLVED
                     MEKLRKHLGIDRWQLLGGSWGSTLSIAYAERYPERVSELLLRGIFLLRRAEIDWFYQD
                     GANWIFPDTYQPFRDLIPPAERADMVAAYYKRLTGPDRRTQIEAARAWSLWEGSTLSL
                     LADPERLKLFGVEAYALAFARIESHYFVNRGFLGRDTQLLDEAKKLAGIPGLIVHGRY
                     DVVTPLKNAWELKAVWPAAELEIIPDAGHAMTEPGIVDAIVAGTRRFAEHVEIQQSGR
                     "
     gene            complement(39218..>40602)
                     /locus_tag="PK264_01085"
     CDS             complement(39218..>40602)
                     /locus_tag="PK264_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74523.1"
                     /translation="QSPLLGTVMACLLVVPLILLLSAELRVGAEAAALLFGRPVAHTA
                     TLLAITIMIVVGASGLRSQTWSGVAMAIAALLAFIVPIAIVATMETFLPIPQLSHGPV
                     LRALGRTEALQTLPQIQLPPLSMTLPGQDLSILSGRFASQTTMLGTLAFALLSLIVAF
                     GVAGSPSLLPRCGAAPSVYDSRKSLGWAAVVLIIMVTTMSAVAVLTRDLVLKQAIGSN
                     PAALASWLRDWIGLGLAQVDASGERVDVTRLAFRRDGILYLLPHAHGLPHVVVAVAVA
                     GAVAASLLAACQAAVALSSILVDDIMFGSRLAAGNETRRLLMARASLGLATLAALAVV
                     ANVQVDPLQLVLWSLCLSAATAFPVMVLSVWWKNLTGRGAMSGALAGFSVTALAMLLA
                     EGGVLALASPLSALLGTPITFAVAIAVSLLAHGPGRAELEIIRDVRVPGGETFVDREV
                     RQERARGRKT"
     assembly_gap    40603..40729
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40730..41070)
                     /locus_tag="PK264_01090"
     CDS             complement(<40730..41070)
                     /locus_tag="PK264_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRD74524.1"
                     /translation="MPVARRQALVNPRLGIYASIVAGVLASLVLVLLIVEELGGGQAG
                     VGLGTWLGLGILALFAAIALATLSSSAHDYFVAGRRVPAGFNGLVLGMSLFGGTGAVA
                     LTGALFLGGFDV"
     gene            complement(41075..41335)
                     /locus_tag="PK264_01095"
     CDS             complement(41075..41335)
                     /locus_tag="PK264_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRD74525.1"
                     /translation="MIERKERKPYWRHTKWQMLATVIPFLAIALVLPMFAERLNMSKL
                     LGFPLGYFLVAHGILALAIVAIASFVNRQDAIDHWHGANEDL"
     gene            complement(41371..42282)
                     /locus_tag="PK264_01100"
     CDS             complement(41371..42282)
                     /locus_tag="PK264_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRD74526.1"
                     /translation="MPKSTPPKARTRFDGAAELHRLMNAGMVVAPGAYDCITARLIEQ
                     AGFPAVYMTGAGTSASYGYPDYGLVTMSEMVENARKIVATIGVPLISDIDTGYGNELN
                     VFRTIEEFERVGVAGVHIEDQGFPKKCGHLDDKEIIPLEDYEAKIRAAAAARRSKDFT
                     IIARTDSRASLGFEEAIRRGNAALAAGADMVFVEAPQTLDEIRAVPKRIKGPCLYNSV
                     QGGKSPDVDMATLQAAGYKLTIVPGLVIRTVLESVDDVLAALKADGRAPASVKGTTVK
                     QYFARFGSAEWDRRRTAFRPGKKEAAE"
     gene            complement(42455..43807)
                     /gene="ccrA"
                     /locus_tag="PK264_01105"
     CDS             complement(42455..43807)
                     /gene="ccrA"
                     /locus_tag="PK264_01105"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HRD74527.1"
                     /translation="MPKSKKSATPAAGPTVAAAASSSLGAPKLVSSKKSLYEIGEIPP
                     LGHVPEKMYAWCIRAERHGEPDSAMQVEVVPTWELDSHDVLVLVMAGGVNYNGVWAAL
                     GTPISPIKDVHKNPYHVAGSDASGVVWAVGSKVKRWKVGDEVVIHCNQDDGDDEECNG
                     GDPMFSPSQRIWGYETPDGSFSQFTRVQDRQLMERPKHLTWEESACYTLTLATAYRML
                     FGHRPHILRPGHNVLVWGASGGLGSFAVQLCATAGANAIGVVSEDDKRDFVMQLGAKG
                     VINRKKFNCWGQLPTVNTPEFNAWMKEARNFGKAIWDITGKGNNVDFVFEHPGEATIP
                     VSVLLVKRGGMVVICAGTTGYNLTMDARYLWMHQKRVQGSHFANLLQAAQANKLVVER
                     RIDPCMSEVFAWDQIPKAHMRMLKNQHKPGNMAVLVNAPTTGLRTFEDVMEASQAKFG
                     "
     gene            complement(43932..44996)
                     /locus_tag="PK264_01110"
     CDS             complement(43932..44996)
                     /locus_tag="PK264_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HRD74528.1"
                     /translation="MRSNRIIHIVGCHCEGEVGDVIVGGVPTPPGATIWEQSRFIAGD
                     QRLRNLVLNEPRGGIFRHINLLVPPKSPEAHTGWIIMEPIHTPPMSGSNSICVTTVLL
                     ETGIVPMAEPETRVMLEAPGGIVEAIATCRDGKVERVRIRNVASFADKLGARIEVAGL
                     GTLTVDTAYGGDSFVLVDAKLLGFALTPDEARDLAETGVKITAAANAQLGFTHPTNPG
                     WSHISFCQMTQPVELVDGVKTGRNTVAIEPGKLDRSPCGTGCSARMAVLHARGELRIG
                     EPFVGRSILDSRFDCEVVAETSVAGRRAIVPTLAGRAWITSLTQLLVDPADPWPEGYR
                     LSDTWPMTRTMTTSGSGVRR"
     gene            complement(45096..>45447)
                     /locus_tag="PK264_01115"
     CDS             complement(45096..>45447)
                     /locus_tag="PK264_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD74529.1"
                     /translation="VVPDIAGWRRERLPRLPDTAWIETPPDWLCEVLSPSTMRIDRTD
                     KLAIYAAFRVRHAWYVDPDARTLEVFELASSRWLIAATFKDADMVATPPFVEQCFRIE
                     RTLAGRSGLNHERH"
BASE COUNT         7569 a        14987 c        15262 g         7423 t
ORIGIN      
        1 aagcgcgcgg cgccgaaagc cggcatcgtc tccaaggcaa aagacgcggc aaagaacatc
       61 ggcgaccgca ccaaggcgac cgtgaaggac gcctatggcc gcgccaagga tgcgattgcc
      121 ccggccccga caacgccggc tccggctccc gcaacgcctg ctcccgcagc tccggcgccc
      181 aagcagtaac acgggctgtc acgcctcaat tcatgagggg cggccggaaa cggtcgcccc
      241 tgtcctctgt ggagagacaa agttccgtcg ctattgggcc ggcgcgactc atcttacggt
      301 cgtcgcacgc aacgctcgag gctgagacgg gaccccatgg atctcctttt ccggatcatc
      361 tacgccgccc acgccagagg cacgcaccac aagctcgccc tcgatggcct ccgctatctc
      421 aaggggccga ccgcggaacg ctggcagcgt gtgctcatga agcacgccaa ggatctgatg
      481 gccggcgcca aggcccccga cgacgagttc aaggatttca agaaccacgt gctccacgtg
      541 cgcgacaatt tctggggcgg cgcgcccgag aaggcccgca gctggtatga tcacctcgtt
      601 gatgtgctcg agagccgcga ctggcggagt gccgcctggt gcgccggcat cctcagccac
      661 tacgtgacgg acccgatcca ccccttccat accggccagt ccgaggccga gaacaatgtg
      721 caccgcgcgg tcgagtggag catcaaccgc tcctacgacg cgcttcgcaa gcaggcggat
      781 gcggaattcg cgggcgagat ggttgtgctc ggcgagggcg ccgattggct cgagcgcgcg
      841 atctgcgacg gcgcaacgcg cgccaacgcc tactacgaga agcttatcgc acattacgat
      901 ttcacgcgcg gcgtcgttga gccgacggct ggcatcgacc aggtcggcaa ccgcatttcg
      961 gccgagctca tcgcctacgc ggcggagctc ttcggcaaag tgctcgaccg cgccatcgtc
     1021 gaggcgcggg tggctccgcc cgacgtcacg ctcaccctcg agaccgtgct cgcggccatc
     1081 gagatgccga tcaaggcgct gcagaaaaag ctcgccgatg cgggtgatcg ccgccaggtc
     1141 gagcgcatgt acgacgagct cgtcgccacc ggtacggtcg agacgaatct cccggatgat
     1201 gatcgcatgg tgcgcgacct ccaccgcgcc gaggtgctgg caaagctgcc gactgcgagc
     1261 atcgagggcc gcttcacgcc gccggtgcgc gagaagatcg agaccatggt cgcccgccgc
     1321 cgtcgcgccg agcgagagac cgtagccgcc tcgttggcgc agaccgtgcc atcggcctcc
     1381 accacggtcg cgatggccgc ggccacatcg gatgccgccg tccctgcccc cgcgtctccg
     1441 gccacgccgc ccgcaccacg agccatggac cggcgccaat cgcagatagg cggctcgagt
     1501 gagcttggtg atgcagacgt tgaaattcgc aagtcactcc ggggcgatgc aggggcgccc
     1561 tcggctccgc cgccggttcc gggctccgac ggaaggccgg cggcgcttcc agcgaccggg
     1621 gcttcacttc tcgcgcgcct cggtgatgtc ggctccgccg tgactggctc cgcggccgta
     1681 gcgccggccg tgcgcgtcac cgcgtcgccg aagacggccg cattccacca attgctcgag
     1741 ccggatggag cggccccggt ggacgcgagc attcgtgagg agcgcgagcc gcgtgccggt
     1801 cgcccgcgca tcgcacgcga tagcgcagtc gtcgacgcac cctcgatcgg cccgaagacg
     1861 gcggacagac tgatcaccgc cggaatccgc accatcggcg agctgatcga tgccgagccg
     1921 gaggccctcg ccgctcgcct ggcgacgcgc cacatcacgc ccgcgatcct ccgcgactgg
     1981 caggaccaga cccgcctcgt gctggcggtt ccggtgctca ggggcacgca cgcgcaactc
     2041 ctcgtcggcg cgggcctcag aacggcggaa gccctgcgcg atgccgagcc ggcgtcgctc
     2101 tgcagcgccg ttctggcgta tgccgcgaac gccgacggcc agcgcatcct tcgccagggt
     2161 gacccgccgg acattgagcg gatcaagggc tgggtggacg cagctcggac cgcactcgcg
     2221 gcctgatagc cggcctgccg aggtggagcc ggcttcgcat ccggctcgcc tctggttata
     2281 gaggccgctc gtctccacct cgaggcttgg atatgcgacc ggctggaaaa ggcccgctcg
     2341 actcgattta tttcacctat ccgcggtttg ccgcgagacg cgcgccggac ctcgatggcg
     2401 tgcaaacgcg ggcgcccgtc gccatcgtcg gtgccggtcc cgtcggcatg acggcggcgc
     2461 tgacgcttgc gcactatggc atccgctccg tgctcctcga tcagaaggac accttcaacg
     2521 acggcagccg cgccatctgc atcgcgcgcc cgagcatgca catcctcgag cgcatcggcg
     2581 ccgtcgcccc gttcctcgac aaggctctcc cctggcgcta cgggcggagc tactaccgcg
     2641 agcatcaggt cttccgcctc gagatgccgc actccgagaa cgaccgctac gagccgatgt
     2701 acaatctgca gcaacagtac atcgagcagt tcctgcatga cgccgtcgcc gcccgtcccg
     2761 agatcgacat gcgctggcag agccgggtcg acgccattcg ccaatcggaa ggcggcgcga
     2821 ccctcaccat tcgctcgccg gcgggcgact acgacctcga cgccacctat gtgctcgccg
     2881 ccgatggtgc ccgcagcccc ttgcgatcgc tgctcgggct gcgcctcaag ggacagaact
     2941 acgagggcaa gtacgtcatc gccgacatcc gcatggcgca cgatttcccg accgagcggc
     3001 gcgccttctt cgagccgcgc gccaatccgg gcagcaccgt gctcattcac cgacgacctc
     3061 tggcggctcg actaccagct gcgcgacggc gaggacgagg cggaggccgt ccgcgaggag
     3121 acgatccgcg cgcgcatcgc cgccatcctc ggcgacatcg gtcacgccgg cccctgggag
     3181 ctcgaatggt ggagcatcta cacggccaac acgctgctcc tcgacgatta ccgcgcgggc
     3241 cgcgtcttct tcatcggcga cagcgcgcac atcgtgccaa tcttcggcgt gcgcggcttg
     3301 aacaacggcc tcgcggatgc cttcaacatc ggctggaagc tcgcctatgt gctcgccggc
     3361 aaggcagggc cagggcttct cggcagctac acgcccgagc ggcgcggggc gacgctcgac
     3421 gtcttcgcca atgcggggaa gagcacgcgc ttcatgacgc cgccctcgcg cggctggcgg
     3481 ctcgcgcgcg aggcggccct gtcgctcgcg atccggcacg ccttcacgcg gccgcttgcc
     3541 aatccgcgcc agatgcaggc ctacacgtat gctgagagcc cgctcacgcc cttcccggcg
     3601 cgtgaccagg agttcgcggg cggaccgcgc tcagggagcc tcgcgccgga tgtacggctc
     3661 caggatggca ctcatttccg aagccgcatc ggcgacgagc tgacaatcct tctctttgcc
     3721 gacgcggcga gcctgccgcg gcataaggcg caagtccgcg ctagtccggg cgccgccgaa
     3781 ctcgcggtgg tgacggtcgt cctcgggccc gattgcgccg acgccgccgc gatcctcgac
     3841 acggaccatc gcattgcccg cgcctatgat gctctcgatg ggaccgtcta tctgctgcgt
     3901 cccgaccatc acatcgccgc acgctggcgg caacttgatc gctgcgagct ggaagcggcg
     3961 atcgccgcct gtctcggcaa ggagacgcca tgagcgctga gcaggatcat ctcgaggccg
     4021 cctacgaagc ccttgcggcc gccattgaca cggcaggcgg cgagaacgag gcgctgttcc
     4081 tcacaaagct cgccctcata ctcgcagacc ggattgccga tcccgccgcc ttcgaggcgg
     4141 ccatcgccat cgcgctcgag gatctgccag gcgccgcgga gcggcactga ggcagttgca
     4201 ggggctcgac gcgacggcgc gtgtcctcta ctgagttggc cgaaccctaa tccacgatcg
     4261 gtcaggagtt ggcgatgagc aaggttgcgt ggctgggcct cggcgtcatg ggctatccca
     4321 tggcggggca tctcgtgaag aagggcgggc acgacctcgt cgtctacaac cgcaatggcg
     4381 ccaagtccgc ggcctgggcg aaggagttcg gcgggcgcac ggcggcgacg ccggctgagg
     4441 ccgccaagga cgccgacttc gtcttctcgt gcgtcggcaa cgacgatgat gtccgcgccg
     4501 tcaccatcgg caaggacggc gccttcgcga gcgtcaggaa gggcgcgatc ttcatcgaca
     4561 atacgaccgc ctcggccaac gtggcgcgcg agctctacgc cgccgccaag gccaagggct
     4621 tcgacttcct ggacgcgccc gtctccggtg gtcaggccgg agcgcagaac ggcgtgctca
     4681 ccgtgatggt cggcggcgac gaagccccat ttgagcgggc gaagccggtg atggctgctt
     4741 tcgcccgcgc cgtgacgctg atcggcacct cgggcgccgg ccagctcacc aagatggtca
     4801 accagatctg catcgccggc ctcgtgcagg gtctcgccga aggcgtgcac atggcgcaga
     4861 aggcgggcct cgacctcgcc aaggtgatgg acacgatctc caagggtgcg gccggctcct
     4921 ggcagatgga gaaccgctgg aagacaatgg ccgagggcaa gtacgacttc ggcttcgccg
     4981 tcgactggat gcgcaaggac ctctcgatct cgctcgatga agcgcggcgg aatggggcga
     5041 cgctccctgt ggctgccctc gtcgaccagt tctacggcga ggtccagcgc cttggtggca
     5101 accgctggga tacctccagc ctgctcgccc gcctgaacaa ccagaagaac gacaagaagg
     5161 cgtgagccag acgagccccg ggccgatgcg gcgcccgagc cccatggagg ttctcaccta
     5221 catggggtcc atcaacccgg caacgcagca tctcgacatc aaggtgctcg cggcggagcc
     5281 gggccgcgcg cgattcgcca tgcttgtgcg tccggacatg gcgaacacct tcggcacctg
     5341 ccacgggggc atcatcttca cgctcgccga taccgcgttt ggatggacgg gcaacgcgac
     5401 caacgagaag gcggtgactg cgggcgcctc gatcgagatc ctggcaccgg ccatggtcgg
     5461 cgaccggctc atcgccgacg ccgtcgaggt gttccgcgag gggcgcaacg tgctgctcga
     5521 cgtgcgcgtc tggcgcgagg gcacggacgt cacggtggcg catgtgcggg gccgcatgcg
     5581 tcaagtgggc ggcgcattgc tcacctgata tatcgcggcg gcccggtctg cagtacgttc
     5641 agcggcagat gtcaccggag gaacgtgcca tgaccgccga gcgcaacgag ccgcgcattt
     5701 ttcgcggcct caaggacgtg tacttcgagc gttcggactg cacgcgcatc gacggcaagg
     5761 cgggcgaact cagctaccgc ggctactcga tccacgatct cgcggcccac tcgacattcg
     5821 aggagacggc ctacctgctg ctcaacgggg cactgccgaa gacatacgaa ctcgcagcct
     5881 tcgatgccgg cctgaaggcc gagcgccagc tgcccgcgcc gattctcgac atcatcggtg
     5941 ccgtcagggc cgggcatccg atggacgtgc tgcggactgc ggtgtccgcg cttgccgcat
     6001 tcgacccgca ggtcacggac aacggccggg ctgccacgat tgccaaggcc atccggctca
     6061 cgagccaagt gccgatgatc gtcgcggcac acgcgcggct ccgcgtcggc aagcccgtga
     6121 tcgcacccga gccggcgctt ggtcatgccg cgaacttcct gtggatgctc aagggcgtgc
     6181 agccttcgga ggacgctgcc aagctgctcg acaccgattt cgtgctgcac gccgaacacg
     6241 gctcgaacgc gtcctcgttc gcggcgcgcg tcgtgatcgg caccgacgcc aacatccacg
     6301 ccgcgatgac ggcggcaatc gccacgctct cagggcccgc gcatggcggc gccgccgagg
     6361 acgtcatgca gatggcgacc gagatcggcg atccggagaa cgtcgcggcc tatgtgaagg
     6421 ggaagcgcgc cgccaaggag gcgatcacgg gcttcggcca ccgcgtctac cgggtggaag
     6481 acccgcgcgc gcgccacatg cgcgaaggcg tgaagcggct ctccctcgag atgggcgagc
     6541 ctcgctggta ccagatcctg cagggcgtcg tcgacgagat gaagccgtat gcccgtcacg
     6601 gcgtgaacgt gaatgtcgat ttctattcag gcgtgatcta ccacctgcac ggcatcccga
     6661 aggacctgtt cgtgccgatc ttcgcggtcg gccgcgtgcc gggctgggcg gtgcaggtgc
     6721 tcgagcagca ggcgaacaac atcctgatcc gcccgttgac gctttactcc gggccggagg
     6781 cacggcccta cgtgccgatt gccgagcggg cctgaaggcc ctacggctga cgcccggcgg
     6841 cagggccacc gggcgtcagg tgaatcgtct gaatgtatca gtggctctcg cgaaccggca
     6901 tgcggtggtc attggcgacc gggggcgcgc caggtgccgg agcgtctgta gtccgatccg
     6961 atgtgaccgt gatgtttcgc tcgaagttca cgaaggtgcg cgcctgcgga tcgtcgaact
     7021 tgtagtgcac gacataagca ccgggcgata ggtttggcag ctcgaggaac acgtggaagt
     7081 agaagtcgga ggtttggcgc tcggccgagt tttcgaggtt cacccggcgg ggtgccgtcc
     7141 atacgacgcg accgccgcgg tcacggatct cgatgctggc gaacagctgg aagtggtagc
     7201 ccgagccgcg cgcactccag ccgaagccga ccggctcgcc gtagacgacc acacgctcgc
     7261 cggtgcggta cgaggcgtca gcgcgcttgt tgaaattgcc gaagccgatc ggctcgccgt
     7321 cgatcaagct gaaattgcgt atgccgcgga agctcgtggc cggcgcgcgg ctcacaggcg
     7381 gcccgttgga cgcggggtgg ggatcggggt cggcgccgtt gacgccgtaa ggcacatgcc
     7441 ccgtacccga tcgggcttcg gccgatgatg tgagcgcgct gcccgccacg gtggcgactg
     7501 cggcgaacgc ggcgactgct gcgaaaaatc ggtacgacat ctgcggtcct cctccgctcg
     7561 ggtcctcggg cgctttcgcc cttgcacgcg tgttggagac tccagaccgg aaaatgacga
     7621 aacgttcagc ttggcatgac gaaaatgacc gccgggttgc cccgacggcc atggttgcag
     7681 tgcgcaaata cggagcaacg aaaggtcaga cctttcgttg cggagtatcg cgcgcagggt
     7741 tggccgcatg cgcagcatgc ctccggcatg acgcggccgc gcgcacgagc agtgtgatca
     7801 gagcaccagc atgccgctct tgtgcatgtc gcccgccgtc atgcccgaga cgccctcgag
     7861 caccacgacc tcgacccact cgccggcgac ccgcgcatag acgtgggagg cgctgccgtc
     7921 gtccttgacg aggacggcgt cgtcgactga ggcgaagctg ccgtcgaccg tgcggctcag
     7981 atccaggacg tcctccttcg agaagtccgt gacccggtcg acgccgaggt gcttgccatc
     8041 ggccatcacg tccgtgaggc tccagcggaa ggtatcggag ccttcgccgc ccgtgagcac
     8101 gtcggcgccg ccgaggccgc gcagatcgtc gtcaccggcg ccgccgtcga gtgtctcggc
     8161 cgctttgctg cccttcatga cgtcgtcatg cgaggagccg acgacgactt cgaagttcgc
     8221 gaccttgtcc tcgcccatgc cgacaatcga cttcttggag aggtcgatca tcatgccgcg
     8281 atcggccgcc gagaagtcca gcgtgtcgaa gcccgaccca ccgttgtagc tgtcgttgcc
     8341 ggcgccggca cggacgacat cgtcgcccgc gctgccctcg acgacatcgt cgccctcgcc
     8401 gtcgacgagc gtgtcgttgc cggcgttgcc cttcatgacg tcgttgcccg cgccgccgat
     8461 cacgacgtcg tcgcccgagt tgccggtgag cacgtcgtcg ccatcgccgc cgtcgaccag
     8521 gtcattgccc ttgccgccgg acatcgtgtc gttgtcggcc tcgccgaaca tctcgtcatc
     8581 accggcattg ccgtacatga cgtcattgcc ggcgccgccg tgcagcacgt cgttgtcggc
     8641 taccgtcgac gcgtgcggct tcacctcgcc cgtgggcttg gtgccgccgt acatgacgtc
     8701 gtcgccggcg ccgccgtcga gcttgtcgtt gccgccgttg cccatcatca ggtcgttgtt
     8761 gccgcggacg agcgaaacat tgatctccga cgcctccgtc aggttcgatg ctccgtgtgt
     8821 cgtcgcgacg accttcaggc tgaagttcgc agagtcggga ccggtcagcg tgagccctac
     8881 gaggtcgtcg gacgacagcg tccacgagcc gtcgccgttg tccttgccgg ccgagaggac
     8941 cgcgccctcc ggcatgttgt cgaccaggat cgacagccgc tccgagccgt cggcatcaac
     9001 cagccggacg gcgatgtcga gcggaatcgt cacccgggca acgccgtcgc cgttcaggac
     9061 gtcatcaccg gaccggccga acatctcgtc ctcgccggcg ccgccgtcga tagcgttctt
     9121 gtcgcggttg ccctcgagat gctcgccggt cttgacgtcg tgaacgtcgg acgtcttgag
     9181 cagcagcgcg tccttgttct tctcggcctc gagacgtgcc ttctcctcgg cttccgcctt
     9241 ggccttcgct tcggcctcga ggcgtgcctt ctcctcggct tccgccttgg ccttcgcctc
     9301 ggcctcgagg cgtgccttct cctcggcttc cgccttggcc ttcgcctcgg cctcgagacg
     9361 cgccttctcc tcggcttccg ccttggcctt ctcctcggcg gccttggccg cctgatgcgc
     9421 acgctcggcc gccgtcggac cggaccgcat cagcgacgcc cggtcgataa gaccaccata
     9481 gctatcgttg tcggcgctat cctcgcggaa ttcgatgcga tcgctgccgc cggtgccgac
     9541 gaccttgaac gcggccttgg tccactcgga cttcgaaggc tcgacagtgc cgacgagctc
     9601 gccgttccag aacacgtgga tcgtgtccga ccctgccttg ctgtcggggc gcttcatgaa
     9661 gtcgaagctg aaggtgtact cggtcccggc ctcggtcgac acgtcctgga aaatgttcga
     9721 ggccttggtg ttgtagtcga gctcggcgat cttgtcgccg tcggcggcct tgagcccgta
     9781 gaagcccttg ccccacacct cgatctcgct gtcggacttc cacccgccaa cggttttctg
     9841 gtgcgaccag gagttggcac cgacgatgcc ctgctcgaag ctgccatcga tcagcatctc
     9901 ggtcggcccg ctcgacgcgg actcggccgt taaggcctcg gccgcggctg cgcgctcggc
     9961 gacaatcgcc tcggccgtgc cagccttcag cgccgccgcg ttcgacgcgc caacgttcac
    10021 gacgatgatc gaatcgtcga agtcgttgtc gccgccgccc ttcaggtcct cgaaggcaat
    10081 gcgcactgtg ccggcatccg gatcgacggt cgtcttcacg tgatcgagct tgtcgccgtt
    10141 gaggcccatc gagcccgtgt cgacgctgtg gaaggtcgag gtgccgtagg ccgactttac
    10201 cgtcgtctcg gcgcccgatg cgctcgtgtg cacgagcttc atctcagcgc cggagttgat
    10261 gttccccgcg ctgccatccg cgttgacgaa cttgaagctg ccggccgtgt cggcgagcac
    10321 ctttgccatg cccggctgtg agaagccgtt cggcacgacg aagaaaccga ccttggcgcc
    10381 ggcctcgagg gatacgtcga cagcgctctt gccggcgagg agatcgcccc ccgaaccctt
    10441 gagggacgca ttcgagaaca cgatcctcac gttcgagatc gagccatcgg cgccgatcgt
    10501 gtacatgccg accgcgttct gatagccggc ggtctcaccg ccgaagacca cggaaccctt
    10561 gtagtcgtcg accatggtca tggacgacac gacgtcgtcc gcgaccgagc ccgacttttt
    10621 tgcgacgcta ctcatctctc ttacctcacc acgtctgcca accgacactc cggatcgcgg
    10681 tcacccaacc acgtgcggcc tgcacccgaa gcgctgccgt cgcttctcat ggaacaacta
    10741 ttgacggcag cgacccggtg cggacatgag ccgactgggc ctgttgttcg atcaggccag
    10801 attaggctca atgttcgaag atcacgttca gtcgtcggct gaattgctgc taaaaattga
    10861 gataaattgc gcgcagtttt ctgtgcctgt tcaatggcat cacgccccgg cgttggcgga
    10921 cgccggggcg aaatgagcgg gggtgagcaa tcgttcagcc gtcgatcatc ccggcatgcc
    10981 ggccggagtc gacggcgaag ggccgcttca cggagactgt cctgttgttc accggatcgg
    11041 tgaagatgaa cgtgatggtg tagatgccgg ggcgggcatt ctccggcagt ttgaagccga
    11101 acgtaatatg tatgttccgc gggtcagtcg tctgccccgt gatatccttc gcgatcggca
    11161 gacggtgcag gatgcggccg ttggcgtcgt ggatctcgat aaccgtgctg agatggaacc
    11221 ggtagcccat cgcgatctgg ctccagccga agttcatagg ctcggcatac aggtggattg
    11281 tctcgccagg gaggtagggg cgcacggtgc gcaccaggaa cttcctgaac ccttcggcct
    11341 tgcgctcggt catggcaatg ttgacgaggc ccgatgggcc cgtgagatga ttgggcagca
    11401 ttgtcggtgc ggctggtggc gggacaatgt cgccgctgcc tggggcggcc tgaaagccat
    11461 tgctcgggat cggccttgcg ccgttgctct gggccgtggc cgtcgttgct gcgagtgcga
    11521 aggcgctgat cgacagcgcg gcggcgagga tcgtcttgcg gaaaccggtg gtcattgttc
    11581 gctctccctg aggtgtctct gttcgacttg cgggccgctc tgtgctgccc ggcacaccag
    11641 ggaaatgctc cgatcagagc ggattttgaa caatgatcac atactttcgg tcatctctcg
    11701 ttaactagtg cggccatcgc tggatcgaag cgcgaagcat tgctagaagg cggcactcct
    11761 cgagtgatga gacccgccat ggccaagacg ccgaaacaag agaccgccac caccgccgac
    11821 ggacgcgact ggggcgccac gctcttcctg ccgaagaccg agtttccgat gcgcgcgggc
    11881 ctgccgctgc tcgagccgaa gatcatcgag cgctggcagc agatcggcct ctacgagaag
    11941 ctgcgcgcca aggcgaaggg gcgcccacgc ttcatcctgc acgatggccc gccgtatgcg
    12001 aacggcaata tccacatcgg ccatgcgctc aacaagatct tgaaggacct cgtgacgcgc
    12061 agccagcaga tgctcggctt cgacagcaac tatgtgccgg ggtgggactg ccacggcctc
    12121 ccgatcgagt ggaagatcga ggaggagtac atggccaagg ggaaggccaa gccgaacctc
    12181 aaggacccgg ccgccatgac ggcgttccgc aaggagtgcc gcgctttcgc cgagaactgg
    12241 atgcgggtgc agagcgagga gttccagcgg ctcggcgtcg tcggcgactg gaagaacccc
    12301 tattcgacga tgacatttcc ggcggaagcg cagatcgccc gcgagctcat gaagttcgcc
    12361 gagacgggcc agctctaccg cggctcgaag ccggtgatgt ggtcggtggt cgaaaagacc
    12421 gcgcttgccg aggcggaggt cgaataccag gactaccagt ccgacatggt gtgggtgaag
    12481 ttcccggtga aggccgcagc ggcggtcacg cagcaccgtg acgccggctc gacatccccg
    12541 atcgccgccc aggtcctcac gggcctcgcc gcggaagcaa cgcgaggacc ggtctcgatc
    12601 atcatctgga cgaccacgcc ctggacgctg ccgggcaacc gcgcgatctc ctaccacggg
    12661 aagatcgcct atggcctcta cgaggtgacg aaggcgccgg acggcaactg ggccagggtc
    12721 ggcgagcgct acgtcatcgc cgacaagctt gcggccgagg tgatgaaagc ggcaaaggtc
    12781 gaggcctttg agcgccgcgg cgtcgtcagt gcggacgacc tctcgcgcac gacctgcgcc
    12841 cacccgctcg cgaaatccgg cctcggcggc tatcagttcg cggtgccgct gctcgacggc
    12901 gaccatgtca cggacgatgc cggcacgggc ttcgtccaca ccgcacccgg gcacggccgc
    12961 gaggacttcg acatctggac cgcctcgacg cgcgcactcc aggcccgcgg catcgatccg
    13021 gcgatcccct acacggtcga cgagaacggc gccttcacca cggatgcgcc gggctttacg
    13081 ggcgagcgag tcatcaccga caagggcgag aagggcaagg ccaacgacgc cgtcatcaag
    13141 gcgctggcgg cggccggcat gatggtggcg cgcggacgcc tgaagcacca gtacccgcac
    13201 tcctggcgct cgaagaaacc ggtgatcttc cgcaacacgc cgcagtggtt catccacatg
    13261 gacaaggagg tgcccttcct cgccgaggcg gccaaggcgc accgcgccaa acgcgcggcg
    13321 gccggcatgg ccgagggtca caaccagccg acgccgaaga ccctgcgcga ggtctcgctc
    13381 aaatcgatcc gcgagacgca gtgggtgccg gcctcgggcg agaaccgcat cgccggcatg
    13441 atcgaggcga agcccgattg ggtgatctca cgccagcgcg cctggggcgt gccgatcacg
    13501 gtcttcgtgc ggaaggcgac aggcgaggtg ctgttcgatc ccaaggtgaa caagcgcatc
    13561 tacgacgcca tgttcaaaga gggcgccgat gcctggttcg ccgatctcga cggcgcgcgc
    13621 ttcctgaagc ccgaccacga tcccaacgac ttcgagaagg tcaacgacgt gctcgacgtt
    13681 tggttcgatt cgggctcgac ccacacctac gtgctcaacg atccccactt tccgggcctc
    13741 atgggcatca cgcggaaggt cgacggcggc cgcgatacgg tcatgtacct cgagggctcg
    13801 gaccagcacc gcggctggtt ccactcctcg ctgatcgaga gctcggggac acgcgcgcgc
    13861 gcgcccttcg acgtggtcct cacccatggc ttcaccatgg acgagaaggg ccagaagatg
    13921 tcgaagtcgg tcggcaacgt ggtggcgccg caggacgtca tgaagaccta cggcgcggac
    13981 atcctgcggc tctgggtcgc cgcctccgac tatgccgacg accagcgcct cggccccgac
    14041 atcctgaaag gcttctcgga gacctaccgc aagatccgca acaccttccg ctggatgcta
    14101 ggaactctct cagttgtcgc ttccggcacc tctgcgggga gccggccgga agcgacggcc
    14161 ggtgaggccg gaaagatgcc cgaactcgag cggctgatgc tgcaccggct cgccgagctc
    14221 gcccccatcg tgcgcaaggc ctacgccgag tacgactacg caaaggtggt gacgtcgctc
    14281 tcccagttca tgaacacgga gctttccgcg ttctacttcg acatccgcaa ggacgcgctc
    14341 tattgcgagg cgccatcgag cccgaagcgg cttgcggccc tcgcctgcgt cgaggagatc
    14401 ttccgcgcga cgtgcatctg gcttgcgccg atcctctcct tcacgacaga ggaggcctgg
    14461 ctcgcacgct acggcgatgc catgaaggag ggctcggtgc atctcgagct cttccccgac
    14521 agtctcgatg gctggctcga cgaggcgctc gcccggaagt gggagaaggt ccgccgcgtg
    14581 cgcagcgccg tcaccgcctc gctcgagcac gagcgcgcgg cgaagcgcat cggctcgagc
    14641 ctggaggcag ccgtgacggt cgccatcgag gaggcgggcg tcgccgaggc gctcaccgcc
    14701 gagcagtggg cggaggtgtt catcaccagc cgggcggacg tgcggaccgg caaggaggac
    14761 atgctcgcgc tgaacgtcgc aagcgggtcc gcgaccggca aggctcacta cagggccgac
    14821 gtcgagccga accacggcgt cgcgggggtc gtcgtccagg tggcggcctt cccgcagccc
    14881 ggctggaaga agtgcgcccg ctcctggcgg ttcacgcagg acgtcggcag cgacgcggcc
    14941 tatcctgagc tttccgcccg cgatgccgcc gccatgcgcg agatcgacaa gagggcctga
    15001 tcgcccttgg gaagcgcggc gcgggctcag cgcggcccgc gcagctcctc gcggcggagt
    15061 ttggccagag cattgaaccc ggcgcggaag cccgccgggg gcacgacaat cgcctgccct
    15121 tcgtcctgat cgcccgcaag cctgatctcg accgggcggc cgtcggcgag gcggctggcg
    15181 atctcggaag cgtgccgtgg tccggcctcc atcacgcagg tgcctgagcg gcacatcgca
    15241 taggcgacgc gcaccagccc gctgccgagc gcaaaggtga tgcctatgtc acccgcgctt
    15301 gtcgcgggtc gcgctccggg ctcgtcgcca agatcgcgct tcggcgctgc tgccggcgtg
    15361 gcgcctctcg cgccgacgat cacgcgccag acaacgcttt cgcggcccgc aaccgcatcc
    15421 atgatgaaat gcgtgctgat gcgcggctcc gcgccggcga cgtcgaccgc agggcccgca
    15481 acgtggagaa gcgcgcaacg ctgccgccgg ccgagggggc cgcatctgac ctcccaggca
    15541 ccgaatcgcc ccgccgggtt cggtggcaac tcatcgagcg aggccgaccg ccagggccac
    15601 gcccacacct cgtccacgtg cccctcgacg agttcgaggg cgcatgtctg ttcgacgacc
    15661 gtggtgcgca tgcgatggcc cgcgaaagtc tcgccgaggc ggctcgcgag gtggcgcacc
    15721 ttgtggcagt tgcgcgtgcg cggcgctggc ctgccgatgc cgtcgtcaga ccggagccgc
    15781 tcccgctcgc cgccgccgag acgcatcatc ggcgcaggtc ctgtgggatg tggtgccgcg
    15841 aatggcgagg gatcgttgcc gtgcccaata cggcgcacga ggccagaatg accgcgatca
    15901 gggggcgccg cgcccggtcg atcgctccga cagcaaaaat cgacatccac taccccggcc
    15961 gccgatgcat tcgccgcaat accgctgagt tgtgaacgct agcacacctg aaggtagtcc
    16021 aggaggcaat tctgcatcgg caggtggccg ccgttcagcg cgccggctcg tgcgtcagcg
    16081 gcgaaagctt cgtccaatga cccatcttgc ggccggcgcg agactcacgt ttgccgtaga
    16141 gatggaggca cacgcccggg tcggcggcga gtgcggacca ggcgttggcg tcggggccga
    16201 tcaggttcac catgagggca tcgctgtgcc ggccgggcga gccgagtggc cagccggcaa
    16261 cggcgcgcat gtggttctcg aactggctga cgccgcaggc ttcgatcgtc cagtggccgg
    16321 aattgtgcac gcgcggcgcg atctcgttta cgacaagcgg ctcaggcgcg tccggcccca
    16381 tgtagaagag ctcgacggcg aggagcccga cgtagtcgag gtgcgtcgcg atgcgccgcg
    16441 caatgtcctg agcgcgcgcg acgacatggg ccggcaggag cgaaggcacg ctcgagcggc
    16501 gcaggattcc gccgccgtgc tcgttgcgcg gcaggtcgta gatcgccgtc gcgccgtcga
    16561 gcccgcgcac gatcaggacc gacacctcga acgcgaagga gaggcgacgc tccagaattg
    16621 cgggcgcgtt gcggatctca gccagtgcat ccgcggcacg gacgccggct gcaagcacga
    16681 cctggccctt gccgtcgtag cctaggcggc gtgtcttgag gatggctcct gccggctcgg
    16741 cgaccattgc ccgctcgaga tcggcaaggc tgtcgacggc gtgaaagggg gcgacgggga
    16801 tgccgaggct ctggatggtg ctcttctcga cgagccgatc ctgcgcgacg cggagtgctg
    16861 cggcgccagg cctgacgggg catgtgcgcg cgagccgctc ggccgtctcg accggcacgt
    16921 tctcgaactc ataggtgatg gcgtcgacag agcgcgcgaa ggcatcgagc gcggccgtat
    16981 cgtcgtagcg cgccttggtg ccctcggtcg cgacgtcgaa ggcagggccg gattcgtccg
    17041 agtagacgtg ggtcttcagg ccgagacggg ccgcggccat ggcgagcatg cggccgagtt
    17101 gtccgccgcc gagaatgccg atgcgcgagc ccgggggcag cggacgcgac gggctgcagg
    17161 atgccggctc agacattggg cttcggagcc tcggcgacac tcgccgtctg ggcctgccgc
    17221 caggcttcga tgcgggcagc gagcgccgcg tccgtgatcg ccaggatctg ggcggcgagg
    17281 aggcccgcgt tcgtggcgcc agcctcgccg atggcgagcg tgccgacggg gacgccggcc
    17341 ggcatctgca cgatcgagag gaggctatcc tggcccgaaa gtgccttgct ctgcaccggc
    17401 actccgagca cggggagcgt cgtcatcgcg gcagtcatgc ctgggagatg cgccgcgccg
    17461 cccgcgcccg cgatgatcac cttgaggccg cggtcgcggg cagagcgggc atagtcgtaa
    17521 aggcgttcgg gcgtgcggtg cgccgagacg atccgcacct catggggcac gccgagcgtc
    17581 gtcaggatat cggccgcccg cgacatggtc gcgaagtccg actggctgcc catgatgatg
    17641 ccgacctgcg gctgcggggc tgtcatgaca tggccgtcct gtcgcgggtg ccaaaaagga
    17701 gtgaacgctt taacggaggg cgtgcggccc tggcaagatc gctcttcgag gttgctctac
    17761 ggatcgggct ggaccggaac cgtgaagccg ccctcggcga cgaggcgggc gaagaggccc
    17821 ttctccgcga cgaggctctg gaaggtgccg cgctcgacga tgcggccctt gtcgaggacg
    17881 aggatctcgt cggcgtcgga gaccgtcgag aggcggtgcg cgatgatgaa cgtggtgcgn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ctcggtgtcg agcgccgagg
    18001 tcgcctcgtc gaggatgagg atcggcgcgt ccttcaggat ggcgcgggcg atggcgatgc
    18061 gctgccgctc gccgcccgag agcgaggcgc cgcgctcgcc gatcacgaac ttgtagccgt
    18121 cgggcttcgc caggatgaac tcgtgtgcct cggcgagctt cgccgcgcgc tcgacctcgg
    18181 cgtccgatgc ctcgggccgg ccgatgcgga tgttctcggc gatcgagcgg ttgaaaaggc
    18241 cggcgtcctg gaagacggtc gccatggact ggcggagcga gacgagcgtc acgtccgaga
    18301 tgtcgtggcc gtcgaccaga atgcggccgc tctcgggacc gcggagccgc tggaggagcg
    18361 cgagtgtcgt cgtcttgccg gagcccgtcg ggccgacgag ggcgaccgtc ttgccggggg
    18421 ccgcctcgaa ggtgaggtcg aatacgccct gcgcgcccgt gccgtacctg aaggagacgc
    18481 gctcgaaggc gacctgcccc tgcacggcgc cgatcggttt ggcacccggc ttctcgacga
    18541 tcgctgcgct ctgatcgagg agatcgaagt aggagcgcag cgtcggtgcc tgctggtaga
    18601 tgcggatgac gaagcccgag agctggtcga gcttaccgat cagcagcccc gcgaaggcga
    18661 cgaaactcac gatctggccg acgctgatct ctcccttctg ggcgaggact gcgccaaccg
    18721 agaagacggc gaccatggtg atcgtcgctg ccgcccgcgt gagtacggtg aggagccccc
    18781 accaggtaag cacgggatac tgggctgcga gcagatcgcg catcaggccc tgcatcgccg
    18841 ccatctcggc ggagaagcgc gcatagctct gcacgaccgt gacgttgccg agcacgtcgc
    18901 cgacgcgacc gtagacgtca ttgtgatagc gctcgactgc ggcctgaccg tcactcgtct
    18961 tgcggacgac gaagaggttc aggaccaggt aggcgacggc gagcgcggcg aggatcgcag
    19021 ccatgcgtgc gtccatgcgg atcgcggtcg gcaccaggag gaggatgccg acaatggcgg
    19081 tgagctgctc gcggaggaac ccgagccaga gccagaagag cgcgtcagtg cccgagagaa
    19141 tcgtgcgcac gacagcgccc gagcccttct cggcgtggta gctgatcggc agcgtgatcg
    19201 ctcgctcgaa ggcgcccgac atggcggcga gccggcggcg atgggcgagg cggtccgagg
    19261 cgacggcgac gacgacgctc gcgaagatgc cgaagagtcc aagccccgcc catatgccga
    19321 tgatcgggaa tgcctcctgc cctttcgaga gcgcatcgac cacccggccg aacaggatcg
    19381 gctcggcaag ctggacaacg gcgacggcga tgccggcgag ggcgagggcc acggcaagcc
    19441 cgcgctcgct cgccagcagg ctcacggcgc gcttgtagac ctggggagtc gtcagggaag
    19501 gtgtcgtcac ggggcggcgc ttctcgggcg atgatcggtg cgcgggatga gttatcgctt
    19561 gcgccagatt cgcagcgtct tgaatggctc ggcctcctcg aatgagcgga gccgcgcgag
    19621 ctcgcgggcc gccgccgccg ccttgtcgta ggcgaaggag ccggaataga agtggatttc
    19681 gccgcctgcg cggaggcgat cggcgagaac cagggtggcc tcgagatcgg tcagcgcgcc
    19741 gtagctgtca ttggcctcgg tgcggtactc gccgaggtgg aagagcgtca cgagatcgag
    19801 ctcgggcaag agcttcccgt cgatctggta gaggtcgccg aagtaggcct tgtaggtgaa
    19861 gccgaggcgc gggttggcga tcaggagctt cacgtagtcg tcgtactcct cgcggctcgc
    19921 ggtgatgccg agtacgtgat tgccggagcc gttcgacgca gtcttcaggc cgatgatgtg
    19981 atggttgccg gtgcccatgt gaaagatggc cttgccccgc attcccgtgg cctcgaggta
    20041 gtcgaggaag tggatgtcgc aggggcactg cttttcatcg aggctccagg tggcattcca
    20101 gtagttgagc ttgggggctg actgggtcat ggcgggctcg cgctcggatg gcttcgggat
    20161 caggcgggtt tcaggacggc gccgccggcg gcgcgatgga ggatgtgggc cgccacacct
    20221 tccggctcgc gagcgggacg ctgatcgagc gtgacgacga tggcgcccgg gcagcactcg
    20281 cgcacgagcc cgaggaggag ggcttcggtg tctgcgtcga gcgcggagaa ggcgtcgtcg
    20341 agcagcagtg ccgccggctt gtgcaccagc acgcgggcga tcgcaaggcg ctgcccgtcg
    20401 ccgatcgaga gctcctggtc ccagcgcgcg ttctcgtcga gacgggcttt gagcgtaccg
    20461 aggccgacct ggtcgagggc cgcctcgatg gccgcacgct cggcgcggat gtcggggtag
    20521 cagagtgctt ccgcgagtga gccgagtggg acataggccc tctgcggcac gaccatgatc
    20581 tcgccggccg gccgctcgat gcggcccgcc ccgaacggcc aaagtcccgt gatcgccttg
    20641 acgagcgtcg tcttgccgag gctcgagccg cccgccacgt gcgtgatggc gccgggcttg
    20701 gccgtgaggc tcgcgctcgc gatcagcggc cgcccgtttg gatcgtcgag gcggagctgc
    20761 gtgagagcaa ggagcgaggc aggtgccgct gcgacagaga tcgccgggga ggcggcagcc
    20821 gcggcatcga cgccgtcggc cgcatcgacg atgtccatga cgcggcgggc ggaggcgtac
    20881 cactcggcga tccggttgta gttgttcacg acccaggaga tcgccgcctg cacctcgacg
    20941 aaggcgccgg cgagctgcgt cgtctggccg agggtgagtt cgcccgagaa gtacttgggt
    21001 accgcaaaga gcagcggtac gacaggcgtc atcgggccca ccgagttggt gatccaggtg
    21061 atgttggcgt gctggcggac aatccggagc cagcgctcga cgaccgtgcg gtagatgccg
    21121 ccgatgatcg cccgctcggt ctcggcaccc ttgaggaggg agatgctttc ggcgttctcg
    21181 cgcgtgcggg tgagcgcgaa gcggaaatag ccttcggccg cgttcttgtt gccgacggtg
    21241 ccgacgagcg ggcggccgat ccaggccatc agcacggacg ccgtgatgcc atagccgagg
    21301 gccagcagca ccatgtaggc cggaatggtg acgccgccga tctcatagga gccgccgacc
    21361 gtccagagca cgccgacgaa gagggcgaag ccgaccgtcg cctgcaggag gccgatggcg
    21421 atatcggtca agggctccgt cgcccagcgc gagtcgtcgn nnnnnnnnnn nnnnngggct
    21481 cggtgcgccg gagccccatg tgatagaatc gatggccgcc aagccaggag ccgacgagcc
    21541 gctcgacgag ccaggcgcgc cagcgcacct ggagcgtctc gcgggcgatg acgatgccga
    21601 cgccgatcgc cgccatgctg gcaacgattg cgaagaacac cggcaccgac tgcatggcgc
    21661 tcgccgcgtc cttgcggccg agcgcgtcga agaaccagcg gttccaccag ttgagggcag
    21721 cctgagcgag cacgccgagg ataaggcagg ccgcgagcga gatggtgagc agccacgcct
    21781 gacgcgcggt tggcccccgc caccagccgc cggaataggc gaggaacgcc ttcgtcaccc
    21841 ggcgatcgag gtgaaagccg gtcgtctcag gcgtcgcgtc ggcggctgcg gtcatgggct
    21901 cggatgccgt ctcggaatga gggcgatcaa tgagaggcgg gcgtcgaggc cgaccgctgg
    21961 cggagggact ggagaaggcc tgctaacgga ttgaatgtga agcggacctt aacgtcgcct
    22021 gccggcacct cgacggcacg gaacagcaga tttgcccgca ggatgggggc cgggaggagc
    22081 gcgccgccct gcacctcgat ctcggcctgc caccaggggt gccagaggtc gttgaggacc
    22141 aggaatccgc cgtcggagct cgttgcctcg atgacgacat cggtgttgcg gtagtcgagg
    22201 atccggaccg tgcccggacg gcgccgccgg ctcgccggca gcggcccttc gagaagcacg
    22261 gttgtgcgcg ggtcgaaatc tggccagcgg ccggacgtga tgattgcctc cggatcgccg
    22321 aggggccgcg cctctatcgc aaacagcacg cgcgggagcg ccgcccggtt ctcgtagatg
    22381 taaccttcgg ccgtgccgac gacgagggtg agttcgcctg gcatggccgc cttgtcgatc
    22441 ttctcgatag ggatcggcgt cgcgatgtat cggaggccca gcaacctggc gagcggcgag
    22501 gcataaccct tgaagagcgg cgcgaacttg cgctggtcgg gaaggccgac ggtgtcctcg
    22561 gcgccggtcg cgcgcgagta gcggccgagc cgcacggggt tgtagccgag cgtcgcctcg
    22621 aggcggtggg tgagcgagag gttcggccag tggaagccga ggccgacgag ttccacccga
    22681 tcacggcgcg tgtcgctctt cccctcgccg gtgagccgct tcagccaggt gatggtctca
    22741 ttgcgggtcg tgggctccat cgcctcgtac atctcgggcg gcatggcgga ggaggtggtc
    22801 ttgccgttgt tccaggcgag atcggcggtc gtgaccacgg cgagggcgag agccgcgaca
    22861 tgcggcgaga gcgcgacgcg cggccaggcc caggcgatga gggcgacggc cagtagcagc
    22921 cagccgccgg cgatcagcag cggtgtccac gcccgctcga gccggtcgag ccgccagccg
    22981 atcgccagca tcaggatgag gcccgcaacg acgagagccg cgactccgag gacgtggagg
    23041 ccccgcggcc gcagccaagg cgcctcgaac atgcgggcaa ctgcatagcc tgagaggatc
    23101 gccgcgagtg cgccgatcac gaaggtcgcg tccgccggcc ggcgatagaa tttcacgccc
    23161 ggcagcacct cgtagaagcc gcggaaaacg ggcgtgtacc agccgagcgc gtagaggatg
    23221 acgagcgcca gcgccaccgt gaagaagcgg atctcgcgcg accagagccg gcccgaaaag
    23281 agcccgagcg cgatcatgag gagcggaatc agcgcgacgt acacctgtcc catgttctgg
    23341 gcgatgtaga ggcccgtatc gtgccaggtg aagctcggcg gaccccagta gtcggccatg
    23401 gcgccggcgg cgccgaagat ctgcggagcg aggaacgtga cgagcagcgc cggatgcagc
    23461 gaaccgcgtc ccgcaccttc gaagtcgatc gctggacggt tcgattccgc ggcgagcgat
    23521 gccgtcagca acaatggcac cgcaaccagc gccacagcga tcaggccgcc gagcatcaag
    23581 ggcaggatcg agcggaggat ggttgcgcgc ggctcgtcgt cggagagcca gcgccagggc
    23641 acgtaggcgg cgagaatgta gaggcaaatg agcgccacct ggtcgcggcc gagcagcatg
    23701 tgggccgcag cgataccggc gagcattcca tagatgaatg aaccacgatc gatggcgcgg
    23761 tcgagccaga ggagtgcaaa ggggagtgcc gcaaggctca gcacctgtcc agtgtgctgc
    23821 atgcgccacg acatggaggc gccgaagcag aacacgaggc ccgcgatcag cgcgcccgcc
    23881 cagtgccact cctggtcacg gaaccagagg gcgagcgcca ccgccccggc gaggcccgaa
    23941 agcagcacgg tcatgtcgac cgcccagagg ctcggcgagg gattgaccag ggcgaggatc
    24001 aggaagggcg cggaaaagat catcgactgc gggtcggcga tctgcggatg gcccgagaac
    24061 acgtagggcg tccaggacgg ccactcgccc gaccagaggc tttgggcgag gaattggatc
    24121 tgcggctgga aatgggcctt tgcgtcccag ggaatggtca cctcgccccg cgcccagggc
    24181 cacacgagta cgatccagag caccgcgagc gcggcaagcg cgatggcata accgtcggca
    24241 tcggcgagac tccaccgctc gcgctcggga ttgaacgctc gccgcggcgg gcggtttgaa
    24301 gatgccatgg gcgctcgtct cgaatggaag ctcagcgaac tggccggcca tgagccgcca
    24361 aacccgcgac ttgtgccacg cgcccgcggc ttggtccaat gctgatgcgg tcctggcctc
    24421 gggcttgtcc cgaggcccat tcatcggcga atgccggaat gtgcggcccg atggggggcg
    24481 gacaaactcg tccatgagaa tgttgctggg ctgagtcttc gcacgggccc aacaattccg
    24541 gtcgcgcccg gagcgttagc taccacacta ctcagcgcca acctgcatgc gaccgcgatg
    24601 ggctcgcgtt tcgcgctcgc tacaaactcg aaagggattg ggctccctgt cgcggatcgg
    24661 gcgcgacgct cgtcaagccc gtggtcagca ttggatgtcg acctggagga gaccggagtt
    24721 tgcctgcggc ttagcggacg atggagatga cggcctcggc gggcacgtgg gcgctcatct
    24781 gggggagggt cgtcgcgatc agagacgcaa ccgcaatcgc tgccagaatg atccgcatgt
    24841 cacactcccc gttgttcgtc cggcttaccc ggatcgctgt caccgaagtg gccttgggcg
    24901 caccagacac tactttgggt gaatgaattc tgaatgagag tttctcctgt ggaaaaggtc
    24961 gcgccgtggc agcagcgtcg tgcgcacggc aaaccggcgc gccttgccgc tcgtcaaagc
    25021 ttgaccgcga cgcgcggcca atggtgttgt tcctgagcca tcagccggag gtcgtgacgc
    25081 gccatggatt tcaccatccc gaaggagctt gccgagctca gagacaaggt cgcggcattc
    25141 gtccgatcga agatcatccc cttcgagaag gacccgcgct ggggccatca cggcattccc
    25201 gaggatttga ggcgcgattt gaatgcgacc gcgaaggagg cgggcctcct cggcatcaac
    25261 tcgccggagg agttcggcgg caagggcttc tcgcacttcg agcaggcggt cgtgttcgag
    25321 gcggcgggct actcgatcct cgggccgatc gcactccatt tccacgcgcc ggacgagggc
    25381 aacatccacc tcctcgacgt ggtcgcgaca ccggcgcagc gcaagaagta tctcgccaag
    25441 ctcgcaaccg gtgaggtgcg ctcgtgcttc gccatgacgg agccggacgg cggggctggg
    25501 gccgatccgc tgctcctgaa gacgcgcgcg acgccggacg gcaacggctt cgtcatcaac
    25561 ggccgcaaga cgctgatcac gggcgcggag ggggcggctt tcaccatcat tatggcacgc
    25621 acgggcgacg ggcccggcga ctgcaccatg ttcctcgcgg agcttcccag caaggccttc
    25681 aagatccacc gcgtgctgaa ctcgatggat tcgagcttca tgggcgggca cgccgtcatc
    25741 gacatcgaaa acctgcgcgt ctcgggcgac gacatcctgg gcgaggtcgg caagggcttc
    25801 cagtacgcgc aggtgcgcct cgcgcccgcg cgccttacgc actgtatgcg atggctcggc
    25861 tcggccaacc gcgcccacga catcgcgact gagtatgcgc ggcaccggaa atcattcggc
    25921 aagacgctcg gcgagcatca gggcgtgtcg ttcatgctcg ccgacaatgc catggacatc
    25981 cacacggcgc gcctcgcgat ctggcattgc gcctggctgc tcgaccaggg cgacaaggcc
    26041 tcgaccatga gttccatggc caaggtgatc tgctcggagg cgatcttccg cgtcgtcgac
    26101 cgctcgatgc agatcctcgg cggctccggc gtcaccgacg acaccatggt cgagcgcatc
    26161 ttccgcgacg tgcgcgcctt ccgcatctac gacggcccgt ccgaggtaca ccgccactcg
    26221 ctcggccggc gcatcgtgca gggcgaccgg aagaagcacg cttgaggctg tgctgcagcc
    26281 tcactccgcg gcgacttgct ccgtgacggt cgggccgtca cccgcgacgg tcgtgcggcg
    26341 catgtcgcgc ggcttcgtct cgtcgaagcg gcgcacgcgg tgcatggtcg tgcggttgtc
    26401 ccacatgacg agatcgaact ggcgccactt gtgggcgtag acgtaggcgc gctgcgtcgc
    26461 gtgctcggcg aggtcgcgga tgaagtcgcg tgcctccggc atcggccagc cgacgatgcc
    26521 gccgatgtgg gaggagaggt acagcgactt gcgcccgctc acgggatgcg tgcgcacgag
    26581 gcgctggcgg accggcttga acatcgcccg ctcgtcctcc gaaagctcgg tgaagcccat
    26641 cgagccgcgc gaatagatga gcgaatgctc actgacgagg tcgtcgcacg tggcgcgggt
    26701 ggcgtcgtcg agcgtttcgt aggcgacgcg catgtccgcg aactcggtgt cgccgccgtg
    26761 gtcggcgacg atacggcccg agagcagcga gtactttgcg ggcgtcgcgc ggaaggagct
    26821 gtccgagtgc cagagccggt tgccgaggtt gaacatgcgc cgccggtcgt tccgctcgaa
    26881 gggcttgccg gactgatcga ggttcgagac gtcgatcatg ccggttttga ggcggtactc
    26941 gcccgccttt gtgatgttgc cgccctcgcg gttctcgatg cggccgaaat tcagcgtgaa
    27001 gtccatctgt tgatcatcgc tgatggcctg accgcggaag acgaggacgg cgtagcggtc
    27061 catgccggcc tcgatcgcgg cgacctcgtc ggctgagagc gacttggtga ggtcggcgcc
    27121 cgagacctcg ccgacgaaca cggggtggat ctggcggatc gagatggcca tggcgacgct
    27181 ccgtggtgag gaccggcaaa gcgtagcggc gcggatgggc cgcctgcaag cgcgaggcgt
    27241 gcagagcagc attgccccgc gccgtcgcgt tatttctggc ggcttgagga gaggatggcg
    27301 gtccggtagc cggcatccgc ggggatagcg acgacccact cgccgttcgc cgactgcgtg
    27361 cgctcggggc ggacgcagac gatgcggcgc gcacgagccc gctccatggc ttcgtggacg
    27421 ctgcggctct cggcgaggag ctcgaggttc aagcgggtcg gtaccacgag cgtgcgtacc
    27481 ttgcgccgcg cttcatcgag gatcgcctcg gggcgcgtcc agaagccgtc gaccatctcg
    27541 cggccatcgt gcatcggttc ctgcccttcg ggcgcggcga cgaggaagaa gtgcgtgtcg
    27601 aagcgcttcg gcaatccgaa cggcgtctgc caatgggcaa agtgcaccat atggtcgagc
    27661 gcgggcgcga gccgctcctg gcggatgatt tcggcgaagg tcagcttgcg atcgaggagg
    27721 gcctcgcgat gcgcgccgac catgcgccgc gcctcggacg gcgcgacgtc gccagttctc
    27781 gtgccgatgg gccgcgcgat gacgatgccg gcctcctcga aggtctcgcg catggcggca
    27841 agccagaagg ggagatcggg atggctctgc ggcggctcga gtgtcgcgcc gctccaggcc
    27901 tcgggcccgt cctccgcgtc gaccttgccg cccggaaaga cgacggcacc catggccccg
    27961 tccatcggcc ggtcgcgcat caccatgtag atttcgatgc cgtcccggcc gtcgcgaacg
    28021 agcatgatgg tcgcagacgg gcgcgccggc acggcctcct gctcgggcgt cgatggctcg
    28081 ctcatgcgtt gttccccgtc gtctcggtcg cgatcggcgc gtggctaaga atggtccgtg
    28141 ggccgtccgg tgacaagcac gcctcacgcc ggatcgatgc gctggaggaa gccggccaag
    28201 tgccgggcgc aggcctcggc gtgtgaatag gggaggccat ggcgcacgcc gtggaagaga
    28261 acgagcgacg cgccttggac acgcgtgcca agctcgcgcg cctgctcgac gggcacgaac
    28321 gggctctccg tcggcatcag gatgagaagc ggcgccttga tcgccgcgag ctgtgtcgaa
    28381 aggtcctgga cggcgaggag ctcgccgagg ccgagcacga cgtgagcggg cgaggccgct
    28441 tgcacgtcag cgaaccattt ggctttttcg ggcgcgagga cgccatcacg gaagcgcagc
    28501 tccatcatgc gggcggccca gcccgccatg ccgtcgcggg cgatctcctc gcgccagccg
    28561 gcgacgcgat ggatgccggt ccccttgaag gctgcattgc tgatcgtgac ggaggcgatg
    28621 cggtccgggc ggaggatcgc ggcgaggagc acgacggtgc cgccgaggga ttccccgacc
    28681 aggtgggcgc gcccggtcgc cccggcggcg agatcgacga cctcgaacag gtcgtcgacg
    28741 agctcctgca tggtccattt gtgcgtgacg tcgggcacgt cggaccggcc gaagccgcga
    28801 aggtcgaagc gcgcgacacc tcgatccggg ccgatcgccg gcagccagcc atcccagatg
    28861 cgatgattgg tgccgatgcc gtgatgaaag acgatcggtc ggcgcggcgt tgtagccgct
    28921 acctccggct cacggatctc gacatgaagg ccgcgtggtg ttcgcatgct cggctcccgg
    28981 atcgcattca agtcgttcga cggcaaatcc tggctgtggt caactggcct cgcaacgatg
    29041 ttccgggtcg ctcgcggagg tctgctcatg aaattcggat acttcaccct ctccgacaac
    29101 cattacccag gcctcggccg ctcgcccgag caattcatcc tcgagatccg cgagcaggcg
    29161 atccacgccg accgcatcgg catgaactcc gcctggatcg gcgagcatca cttcgacacg
    29221 ctcggcgtca actcgtgccc gcacatgctg ctcgccagca tcgtgccggt gacgaagcgt
    29281 atccgcctcg cgccggccgt tgccgtgctg ccgctgcatc acccgctgaa ggtcgccgag
    29341 gagtgggcca cgctcgatct catctcgggc gggcgcgtcg atttcgcagt cgggcgcggc
    29401 tacgaccggc gcgagtacgt gccgttcggc gccgactaca tgtcgtcgct ggagatcctc
    29461 gatgaaagcg tcgaggtgat ccgcaaggcc tggacctcac cgggtccgtg gtcgcacaag
    29521 gggcgcttct tcgacatccc cgagatgcag atcacgccgc ggccgttgca gaagccgatt
    29581 cccttctaca tggcctgctt ctcgccctcc tcgatggaaa tcgcggcgcg gcgcggcctc
    29641 gacatcatct tcgcgccgtt tgcggctcag atgaccttcg gcgggctcgc cgaggcggtc
    29701 gcggcctatc gcgcggcgtg cgccaaggcc ggcaccagac ccggccgcgc catgtgcagc
    29761 tatttcatcc acatcgccga tacgcccgcc gaggacgact tcgggcgcga gagcctgatg
    29821 cgctatttcg agcacgcggt gcggccggcc ttcaagcagg agcccggcaa gaccccgccg
    29881 accatggcct acttccagaa gatcaacgag gtactggccg gcatgcgcaa ggagagcctc
    29941 tccgagaggt ccatccttct cgggccggcg cggcgcatca cagaggtgct caagagcgtc
    30001 gaggccgcgg gcatttccga ggtgatcctc tacttcaacg tgggcggcaa gccgcacgac
    30061 ctcgtgctcg gccagatgga caagttcatg aaggagatcg cgcctgcgtt cagctgaggc
    30121 gagccttcca agtcccgctg gcgaatgcag cctgcgatct tgaccgcgcg atgttgcgcc
    30181 cgcataaact cggccagcat gccagcgtgg tcccgtagct cagctggata gagcatcggt
    30241 ttcctaaacc gagggtcgca ggttcgaacc ctgccgggat cgccaatcca accgggatgc
    30301 cgtgcgccca ttgacagggc cgtcgctcgc ggtcgaaggt tcaggctacc cttctccgga
    30361 gcttgcccca tgcctttcga gcccgccagc gacgtgctct cgagctacac ggtcctcgat
    30421 ctgacgcgcg tgcgctcagg gccgacctgt gtccgccagc tcgccgactg gggagcgaac
    30481 gtcatcaaga tcgaagaccc ggagagcgac aaggtcggtc tcggcggacc acgttcggga
    30541 agcgacttcc agaacctgca tcgcaacaag cgctcgatca cgttgaacct gaagtcgccg
    30601 gaaggcgccg aaatcttcag gaagctcgcc gcaaaagccg acatcatcgt cgagaactac
    30661 cggccggacg tgaagacgcg gctcggcatc gactatgccg caatcgccaa gctcaacccg
    30721 cgcatcatct acgcctcgat ctcaggattc ggccagacgg ggccgctcgc caagcggccg
    30781 ggcttcgacc agattgctca ggggatgggc gggctcatgt cgattacggg cgagccgggc
    30841 aagggcccga tgcgcgtcgg catcccgatc gccgacctca ccgccggcat tttctgcgcg
    30901 caaggcattc tcctcgcgat catcgagcgt gagcggagcg gcaaaggtca gtgggtgcag
    30961 acctcactct tgcaggcaca ggtcttcatg ttggactttc aggcctcgcg ctggctgatg
    31021 gatcaggacg tgccgggcca ggccggcaac gatcatccga cctcgatccc gacgggcgtc
    31081 ttcccgacat cggacggtca tatcaacatc gcggtcgcgg ggcaggcgat gtggcagaag
    31141 ttcaaggcgg tactcgccga tccggccctc gagcacgccg actatgccga cggcaagtcg
    31201 cgctcgaaga accggaaggc gctcaacgcg ctgatcgccg cccacacggt caaggcctcc
    31261 tcggccgaat ggatcgagcg cctgaacaaa gccggatgcc cctcgggcga gatcaacacg
    31321 atcgacaagg tgttcggcct cgagcaggtg cgcgcgctcg gcctcgcgca gccgatggtg
    31381 agccaggagc gcggcaagac cgaggtgctc ggccagccga tcctcatgag ccgctcgaag
    31441 gcgagcgtga aggtgccgcc gccggcgctc ggtcagcaca ccgccgaggt gctcggcgag
    31501 atcggctata ccagcgccga tgtcaccaag ctgaagaaga gcggtattgt ttgagggccg
    31561 ggcgaagtac agcttggctc gcccaatcac catccatctg gagtgcccat ggccgaagcc
    31621 cccgccgagg agaagctcct ctcgcgccgc gatggcgctg tcgcccacat catcttcaac
    31681 aatccggcgc gccacaacgc cgtctcgctc gagatgtggc agcgcatgca catgctgctg
    31741 gatgagctcg ccgccgaccc gggcgtgcgc atgctggtcg tcagtggtgc gggaggcaag
    31801 gccttcgtgt cgggcgccga tatctcgaag ttcgagagcg agcgggcctc cgaggaggcg
    31861 gtcaagctct acaacgcgac gagcgccggc gcctacgaga agctctatca cttcccgaag
    31921 ccgaccatcg ccaagatcca gggctactgc gtcggcggcg gcatgaatct cgccgtctgc
    31981 tccgatcttc gcatcgccaa cgaaagtgcc aagttcgcga ttcccgcagc gaagctcggg
    32041 ctcggttacg ggctgccggg cgtgcgccgc ctctccgaga tcgtcggcat ctcgcgcacc
    32101 atggaactct tctatacggc gaaacagtta tccgcccgtg aggcgcattc gatcggcctc
    32161 gtcaacgagg tcgtcccgga cggcgagatc gatgccgccg tcgagcggct gacggccact
    32221 atcgccggca atgctccgct cacgatcgcc accatcaagg cctgcgcacg cgagctcgga
    32281 aagcctgaat cgcaaaggga tcttgccaag ctcgaccgca tggtgcaggc ctgcttcgag
    32341 agccgcgact acaccgaagg ccggcgcgcg ttcatggaga agcgcaagcc cgtcttcgag
    32401 ggtcgatgag cagaggcgct gccgtgcaag cggtgtctct cccggccggt gctgatcgca
    32461 gctcatgcga cgtgatatcg aaagttcgcc acactcgcgc gcaatagagg cgagccgtga
    32521 tgactgggtt tggggagagg ccgccgaggc gtggcgggcg gtgatcgatg cgtaactcca
    32581 ttgcggtgat gaacaccaag ggcggcgtcg gcaagtcgac gattgtgctc gccttggctg
    32641 aaacgctctc cgcctttcac ggcaagaacg tgctggtgat cgattccgac agccaggcga
    32701 gcgtctccgc catgctgatg tcggtgtcga acctgcgccg cctgcagggc gaaggccgga
    32761 cgatggtgga ctatctgatc gccacggtgc tcaacaacga gccggtcgag tggccgaact
    32821 acgtggtgag cggcgtctcg gacgtcgacg atgcgcgcac ggtctacctg atgccgagcg
    32881 atatgcagct cacccttttc gagcgcgagg tctccaagga atcgctgcat tcgcggctgc
    32941 gcacctcgat caacgggctc ctcgcccgcg cccgcatcgt cttcgacgtc atcctgatcg
    33001 actgcccacc gggcctctcg gtgctgaccg agagctggct ccgcgaagcc gatttccacg
    33061 tctcgccgac gaagcccgac tacatctcga cgtgcggcct cgacgtgttc aaccgcttca
    33121 gggggctcaa tccggagatg ggctttgcga ccaacctcgg cgtcctcgtc aacatgaagg
    33181 atccgatgtc gcaggccgac gaagagtatc agcgctggct gcagcagaac cctgagaaca
    33241 agtgcttcac ggcggtgttg ccgcgcgtca acgcgctcca gcacacggtg cgcttccgcg
    33301 atcaggatcg ctcgttcatg gcgaagtatc cgggcgacgc cggcgcagcg gtccgcgcgc
    33361 tcgccaacga gctgctcaca cgcatcgcca ccgagcaagc gccggccaac ctcacaccca
    33421 aggctgcgac gtcggtcgcc tgacgaagat tgcgaagcct tgcgcgggca gcttcagatt
    33481 ttgcagtccc gcgccggctg cgtcataagg atatcctgga cgcgaatgat ctgcgacttg
    33541 aaagactgaa gagcctgttt gggaggacac gacgaaatga aaagttcgaa gatccgcgaa
    33601 gcaaagcggc tcgcgttgag cggtgcggcg acggctgccg tcgccctcgg tttcgccatg
    33661 agctctccgg cgacagcgca ggtggtcgac gggcccgtcg tcaactggaa ccactcggtg
    33721 tggggcaagg cgcgtgccta cacggccggc atggagaccg tcgtcgccta cctcaaggag
    33781 aagaccggcg gcaagttcac gatcaagatc cactacggcg aggccctctc caaggacaag
    33841 gagaacctcg acggcatcaa gatcggcgcc ttcgagtcgg ccgacttctg caatttctac
    33901 cacccgggca agaaccccgc gtggatggtc ttcacgctgc ctttcctgcc gatcggcgac
    33961 tgggacgtct cgcgcgacgt gcgcatgaag atgctcgagc atccggcctt cgtgaaggac
    34021 atggaccagt ggggcgccat ggcctacatc tcgggtctgc tgccgcagta cgagttcctc
    34081 ggcaagggca cgccgccaaa gaccctggac gactggaagg gccggcgcgt gcgcgcgggc
    34141 ggcggcgtcg gcgacgcgat gcgcctcctc ggcgcggtgc tctcgaccgt tccagcagcc
    34201 gaggtctaca ccgcgatcga gcgcggcacg gtggatgcgg cctcgttccc ctacacgtac
    34261 gcccacgcct cctaccagat ccacaccgtg accgactggt tcacctccaa catgtcgccg
    34321 ggcacctccg agtgcgcggc cgtgttcagc aaggaggcct tcgccaagct gccggacgcc
    34381 tacaagaagc tgttgatgga cgcgcgcgcg ccggcctann nnnnnnnnnn nnnnnnnnnn
    34441 accaggacgt cgacaaggtc aacctgccgc tgttccgcgc acgcctgaag gaggtcgtct
    34501 acacgcctga ggagctgaag gtcttccagg acaaggtcgg ccggccggtg tgggacaagt
    34561 gggtcgccga caacaaggcg aagttcgacg ccaagaaggt gctcgacgat ctgctcgccg
    34621 ccatcgccgc cgcggaagcc aagtacaaga agaaaacttg acaatccggc gccggagtcg
    34681 cgaggctccg gcctagcgta ctcttcggcc cgagcggcac attgtccgct cgggcatcgt
    34741 tttttcactg cgacggcgtc gggggacgtc ccatggcaca tgcgaaagcg gtcgatccga
    34801 ccgcgacaac cggtggcggg ctgctcggcg cgttcgaccg aggtctgcac tggatcgaga
    34861 agggactcgc cctcgcctcg ggcttcggca tcttcgcgct gatgatgatc ggcgtcgtgc
    34921 agattctgtc acgcaagttc ctcaatctgc cgatcttcgg ctacatcgac atggtcgaga
    34981 tcgcgatggc gttcctcgtg ttcctcggcc tctcctatac cgagcgcctc ggcggccaca
    35041 tccgcatgga gctgatcgtc gccaagctca aaggccgtgc gctctggctg ttcgagatca
    35101 tcggcgtcac gtgcgggctt atcatcgtcg gcgtgctcac cgtcttcagc tggcagcacg
    35161 ccatgcgcgc ctataactcc ggcgatagct cgatcgatgc ccagttccag ctctggccgt
    35221 cgaaaatggt cgtttcgatc tcgctcgggc tcctcttcct tcgcctgctc gtgtcgctgt
    35281 gggcctacat ccggctgctg atctcaccgg atgcggcaca ggtcgccgtc atcgaggtcg
    35341 tcgattccga ggagcaggca cgtcgcgaag cggaggccgc cttgcaggcg acgctcggcg
    35401 acgatcctgg ccttgccgcg cctgctgctg ccggtcctgc caccctgaat tcatcccgga
    35461 ccaacgccag ctcaggcccg cagtcatgac cgagttctcg aaagacgcca tcggcatcat
    35521 cggatgcctt gccctgctcg tcctcgtttt cgcgggtgtc agggtctatc tcgccgcggc
    35581 cgtcgtcggc ttcctcggca tggcggcgat gctcggcttc tatcgcagcg ccgccatcct
    35641 cggcacgatc ccgcactcga aatcggtgag ctacaccctc tcggtgctac cgctcttcat
    35701 cctgatcggc tatctcgcct accatgcggg gatgacgcag gcgctgttca gggcggcgaa
    35761 ggcctggatc ggctggctgc ccggtgggct cgcgatcgcc tccatcatcg cggtcgcggc
    35821 cttctccgcc gtgtccggcg catcgacggc cgccgccgcc gtgttcgccc gtgtcgccat
    35881 tccggagatg ctccaggcag ggtacgacaa gcggcttgcg gcgggcgtcg tcgcagcggg
    35941 tggaacgctc gacgccctca ttccgccgtc cgccttgatg gtcgtctacg ccatcatcgt
    36001 cgaggcctcg atcggcaagc tgctggttgc cggtttcatc ccgggcttca tctcggccgc
    36061 ctgctacggt ctcatcgtga tcgtgtggag ctggtggaac ccgagcgtcg gcacccgcct
    36121 caccggctac acttggcgtg agcgcttcgt gtcgctgccc ggcacctttc cggtcgtgtt
    36181 cgtcgtcgtg atcatcttcg cctcgatgta ctacggctgg gcgacgccga cagaagcggg
    36241 cgcgctcggc gcgttcgtcg tgttcctcgc ggccctgctc aaccgggtga agttcgcgca
    36301 ggtcaatgac gcgctcctcg agacggcgaa gctcacggtg atgatcttcg cgatcatctg
    36361 gggcatcctg atcttcgtgc gctttctcgg ctactgcggc gtgcccgagc gcttctccga
    36421 gtgggtggtg gcgctgccgt tcccacgctg ggtgatcatg ctcggcatcc tcggcgtcta
    36481 tttgatcctc ggctgcttca tggacgcgat cggcatgctg ctgctgacga tcccggtgct
    36541 ctatccggcg gtcatcaagc tcggctacga tccgatctgg ttcggcatca tcgtcgtcaa
    36601 gatgtgcggc gtgtgcctgc tgaccccgcc gatcgggctc aactgtttcg tggtctcggc
    36661 ggtaaggccc gacatacccc tatcgacggt attcaagggc gcgctgccgt tcgtgatcgc
    36721 cgacttcatt gcgatcggcc tcttcatgat ctggcccgaa atcatcacct ggctgccgga
    36781 ccaggtcatc aagtagcgcc gggacggacg cgatgagcga ccccggtcag ccgcgccgag
    36841 gagcagccga gttgccgccc gcgcaatcgt cgacggacgc tggcgggatc ctggtcgcgg
    36901 tgatcgtcct cacgttcctg atgaatacca tcgggcgggg gctgaccgag accttcgcgg
    36961 tgtttctgct gcccgtgcag acggggctga acgcgacgcg cgccgagatc tcggcgacct
    37021 actcggtgta catgctggcc tacgccttcg cggcgccctt tgccggtcag ctcatcgatc
    37081 ggcttggcgc ccgtgtcacc tacggcctgg gcctctcagc gctcgggctc ggctatgcgc
    37141 tcggtggtct ctcgacggag gtctggcact actatctcgc catcggcctc tgcggcgggc
    37201 tcggtgcggc agcgctcggc atggtcgccg cctcgagcct cctttcacgc tggttcagcc
    37261 ggcgcatggg ctcggtgatg tcggcgccct atgccgcggt cggggccggg atgctgatct
    37321 ttccgccagt gacgcagctg ctgatcgatt ggctcggctg gcgctcggcg ctctatgtgc
    37381 tcggccttgg cacgctcgcc attctgccgc tgacgctcgc gctcccgctt ggccgcatga
    37441 cggccggctc gagcgagtgg cgggcggcgc gtgaccaggc gcagcgcgcg gccgggggat
    37501 ggacgcttac cgcagcgagc cggacaggcg ccttctgggg gctctttgcc gcctatttct
    37561 ggacgtcggt tgccgcctac gcggtgctgc cgcagtcggt cgcctttctc gtcgagcagc
    37621 gcttcgatcc actggtcgcg gccgccgcat tcggcgtgac ggggagcctc tccgtgtgcg
    37681 gcatcctggt cgtcggctgg ctggccgacc gcatcggccg cctgccgacc gtgacaatga
    37741 cctacctcat gtcgatcgcc ggcacgctcg cgctgatcgg cgtcgcggtc tggccgtcgc
    37801 tggcgctcgt ctacacgttc gtcgtctttt tcgggctggt gcagggggca aggggtccga
    37861 tcatcctctc cctgctgccg acgctctatc cgggcggcgc ggtcggcgcg atcttcggcg
    37921 cgctctccat ggcgctcggg ctcggggccg ggctcggatc gctcgcatcc ggatatctgc
    37981 gccagctgac gggcggctac accgcatcct tcgcgctagc ggccctcgcc agcgcactcg
    38041 ggctcctcac attctggatc gtgccgagcc tgcgccacga gcgcctcggc ggcaaggggc
    38101 cgccgccgca gacgacgcga cctctcacat gagccgcgtc agcggcccga ttgctggatc
    38161 tcgacgtgtt cggcgaagcg gcgcgtgccg gcgacgatgg catcgacgat gccgggctcc
    38221 gtcatggcgt ggccggcatc cgggatgatc tcgagctcgg ccgcgggcca gactgccttc
    38281 agctcccagg cgttcttcaa tggcgtcacc acgtcgtagc gcccgtgcac gatgaggccc
    38341 gggatgccgg cgagtttttt agcctcgtcg aggagttgcg tatcgcgacc gagaaagccg
    38401 cgattgacga agtagtggct ctcgatgcgc gcgaaggcga gcgcgtaggc ctcaactccg
    38461 aagagcttca gccgctccgg gtcggcgagc agcgagaggg tcgagccttc ccacaggctc
    38521 caggcgcgcg ccgcctcgat ctgggtgcgg cgatcgggtc cggtgagccg cttgtagtag
    38581 gcggccacca tgtcagcgcg ctcggccggc ggaatgagat cgcggaacgg ctggtacgtg
    38641 tcagggaaaa tccagttcgc accgtcctgg tagaaccaat cgatctcggc gcggcgcagg
    38701 agaaagatgc ctcgcagcag gagctcgctc acgcgctccg gatagcgctc ggcataggca
    38761 atcgagagcg tcgagcccca cgagccgccg agcagctgcc agcgatcgat gccgagatgc
    38821 ttgcgcagct tctccatgtc ctcgacgagg tgccaagtgg tgttggcctc gaggcaggca
    38881 ttcggcgtcg accggccgca gccgcgctgg tcgaagagga cgatgcggta gcgcgaggga
    38941 tcgtgatagc ggcgcatggt cgggttcgag ccgccgcccg gccctccgtg cacgacgagc
    39001 acgggtttgc cgtgacggtt accgctttcc tcgtaataga ggacatggcc atcgccaacc
    39061 gccagccgat cctgccgata gggctcgatc ggcgggtaga ggccgcggcg gcgtcgatcg
    39121 gatgtggtcg ttccttcccg catgtgccga gattgccagt ttttcgttgc agcgtcttgt
    39181 tcgttcgggc ccgcctgctc cgcgttccct gctgggctca ggtcttgcgg ccgcgtgccc
    39241 gctcctggcg gacttcgcga tcgacgaatg tttcaccccc gggtacgcgc acatcgcgaa
    39301 tgatctcgag ctcggcgcgg ccggggccat gtgcgagcag gctgacggcg atcgccacgg
    39361 cgaacgtgat gggcgtgccg aggagggccg agagtgggct cgcgagcgcc agtaccccgc
    39421 cctcggcgag cagcatggcg agcgccgtca ccgaaaatcc ggcgagcgca cccgacatcg
    39481 ccccacgccc cgtgaggttc ttccaccaca cgctcagcac catgaccgga aaggcggtcg
    39541 ccgccgagag gcagagtgac cagagcacga gctggagcgg atcgacctgc acgttggcga
    39601 cgacggccag cgcggcgagc gtcgcaaggc cgaggctcgc ccgggccatc aggaggcgcc
    39661 gggtttcgtt gccggcagcc agtcgcgagc cgaacatgat gtcgtcgacg aggatcgagg
    39721 agagtgccac ggccgcctgg caggccgcga ggaggctagc agccacggca ccggcaaccg
    39781 caacagcgac cacgacatgc gggagaccgt gcgcatgtgg cagcaggtag aggatgccgt
    39841 cccgccggaa ggcgagcctc gtcacgtcga cccgctcgcc ggaggcgtcg acctgcgcga
    39901 gcccgaggcc gatccagtcc ctgagccacg aggcgagcgc ggcgggattg ctgccgatcg
    39961 cctgtttcag gaccaggtcg cgcgtcagca cggccaccgc cgacatcgtt gtcaccatga
    40021 tgatgagcac cacggcggcc cagccgagcg acttgcgcga atcatagacg ctcggggccg
    40081 cgccgcagcg cggcagcaat gaaggcgatc ccgcgacgcc gaaggcgacg atcagcgaca
    40141 gcagcgcgaa ggcgagcgtg ccgagcatgg tcgtctgcga ggcgaagcgg cccgacagaa
    40201 tggagagatc ctggcccgga agcgtcatcg agagcggcgg cagctggatc tgcggcagcg
    40261 tctgcaatgc ctcggttctc ccgagcgcgc gcaggacggg accgtgactc aactgcggga
    40321 tcggcaggaa cgtctccatc gtcgcgacga tcgcaatcgg cacgatgaac gcgagcagcg
    40381 cggcgatcgc catggcaacg ccagaccagg tctgcgatct gagcccgctc gcaccgacca
    40441 cgatcatgat ggtgatcgcg aggagcgtcg cggtgtgggc aacgggcctg ccgaagagca
    40501 aggctgcagc ctccgcgccg acccgcaact cggcgctcaa cagcaggatc agcggcacga
    40561 ccaggaggca ggccatcacg gtgccgagca gcgggctctg gannnnnnnn nnnnnnnnnn
    40621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna catcgaaccc
    40741 gccgaggaac agcgcgcccg tcaacgcgac ggcgccagtg ccgccgaaca gcgacatgcc
    40801 gagcacgaga ccgttgaagc ctgccggcac gcgccgtccc gcgacgaagt aatcgtgggc
    40861 cgagctggag agggtcgcga gcgcgatcgc cgcaaagagg gcgagaatgc cgagcccgag
    40921 ccacgtcccg aggccgacgc cagcctgtcc gccgccgagc tcctcgacga tcaggagcac
    40981 cagcacgagg ctcgcgagca cgccggcgac aatgctcgcg tagatgccga gccggggatt
    41041 taccagcgct tgccgcctcg ccacaggcat cgggctacag atcctcgttc gcgccgtgcc
    41101 agtgatcgat cgcatcctgt cgatttacga acgaggcgat cgcgacaatg gcgagggcga
    41161 ggatgccgtg agcgaccagg aagtagccga gcgggaaacc gagcagcttg ctcatgttca
    41221 gccgctcggc aaacatcggc aacaccaggg cgatcgcgag gaagggaatg acggtcgcca
    41281 gcatctgcca cttggtgtgc cgccagtagg gcttgcgctc cttgcgctcg atcatctttc
    41341 aacgcccctg tcctcggcgc gacgcgccac ctactccgcc gcctccttct tgccgggcct
    41401 gaaggcggtt ctgcggcgat cccactcggc ggagccgaag cgggcgaaat actgcttcac
    41461 cgtcgttcct ttgacggagg caggtgcgcg cccgtcggct ttgagggcgg cgagcacatc
    41521 atccaccgat tcgaggaccg tgcgaatgac gagacctggg acgatggtga gcttgtagcc
    41581 ggccgcctgc aaggtcgcca tgtcgacatc gggcgacttg ccgccctgca cggagttata
    41641 gaggcacggg cccttgatgc gcttcggcac ggccctgatc tcgtcgagcg tctggggcgc
    41701 ctccacgaac accatgtccg cgccagcggc gagggcagcg ttgccgcgcc tgatcgcctc
    41761 ctcgaagccg agtgaggcgc ggctgtcggt gcgcgcgatg atcgtgaaat ccttggagcg
    41821 gcgcgcggcg gccgcggcac ggatcttcgc ctcgtagtcc tcgagcggga tgatctcctt
    41881 gtcgtcgagg tggccgcact tcttcgggaa gccctggtcc tcgatgtgta cgcccgcgac
    41941 gccgacgcgc tcgaactcct cgatcgttcg gaacacgttg agctcgtttc cgtagcccgt
    42001 gtcgatgtcg gagatcagcg gcacgccgat ggtggcgacg atcttcctgg cgttctcgac
    42061 catctcgctc atggtcacga ggccgtaatc aggatagccg tacgacgccg atgttccggc
    42121 acccgtcatg tagacggccg ggaaccccgc ctgctcgatg aggcgggcgg taatgcagtc
    42181 ataggcaccg ggcgcgacca ccatgccggc attcatcagc cggtggagct cggcggctcc
    42241 gtcgaaacgc gtgcgggcct tcggcggtgt cgatttcggc atggcatcct cacgggcaaa
    42301 tggcgagcat gcggtcaagg accgcagcac tcatctgccc ccgatgcccg ccggagggca
    42361 aggcccgata tgacatacgt gggggcgcgc agctggaccg gcgcccccag gaactcgcag
    42421 cactggacga caaggtcacg tcaagggtgt tggatcagcc gaacttcgcc tggctcgcct
    42481 ccatgacgtc ctcgaacgtc ctgaggccgg tggtcggcgc gttgacgagc acggccatgt
    42541 tgccgggctt gtgctggttc ttcagcatcc gcatgtgggc cttcgggatc tggtcccagg
    42601 cgaacacctc agacatgcat ggatcaatgc gccgctcgac gacgagcttg ttggcttgcg
    42661 cggcctgcag gaggttcgcg aagtggctgc cctggacgcg cttctggtgc atccacagat
    42721 agcgcgcatc catggtaagg ttgtagccgg tggtgccggc gcagatcacc accatgccgc
    42781 cgcgcttcac gagcagcacc gagaccggga tcgtcgcctc gcccggatgc tcgaagacga
    42841 agtcgacgtt gttgcccttg ccggtgatgt cccagatcgc cttgccgaag ttgcgcgcct
    42901 ccttcatcca ggcgttgaac tcgggtgtgt tcaccgtcgg cagctggccc cagcaattga
    42961 acttcttgcg gttgatgacg cccttggcgc cgagctgcat cacgaaatcg cgcttgtcgt
    43021 cctcggagac gacgccgatg gcgttggcgc cggccgtcgc gcagagctgc acggcgaacg
    43081 agcctagtcc gcccgaagcg ccccacacga gcacgttgtg gccgggacga aggatgtgtg
    43141 ggcgatggcc gaaaagcatg cggtaggcgg tagccagcgt cagcgtgtag caggcgctct
    43201 cctcccacgt gaggtgcttc ggccgctcca tgagctggcg gtcctgcacg cgcgtgaact
    43261 gtgagaacga gccgtcgggc gtctcgtagc cccagatgcg ctggctcggc gagaacatcg
    43321 gatcgccgcc gttgcactcc tcgtcgtcgc cgtcgtcctg gttgcagtgg atgacgacct
    43381 cgtcaccgac cttccagcgc ttcaccttgg acccgaccgc ccagacgacg ccggaggcat
    43441 ccgagcccgc aacgtggtag gggttcttgt gcacgtcctt gatcggcgag atcggcgtgc
    43501 caagcgcggc ccacacacca ttgtagttga cgccgccggc catcaccaga acgagtacgt
    43561 catggctgtc gagctcccag gtcggcacca cctcgacctg catggccgag tccggctcgc
    43621 catggcgctc ggcacggata caccaggcgt acattttctc gggtacatgc ccgagcggcg
    43681 gaatctcgcc gatctcgtag aggctcttct tcgacgagac gagctttggc gcgccgagcg
    43741 atgaactcgc agctgcagca acagtcggcc ctgccgcggg tgttgctgat ttcttcgatt
    43801 tcggcatggt tcatcccctc cggtgccgcg tatgcgcgtg acggaatatt gcattgcagc
    43861 aagaggctat accagtccac tttggactga tccagttttt ctgcgacgca tgaggatcgc
    43921 ctgcactgta gttaacgccg cacgcccgag cccgacgtcg tcatcgttct ggtcatgggc
    43981 caggtatcgg acaggcgata gccctcgggc cagggatcgg cgggatcgac gagcagctga
    44041 gtgaggctcg tgatccaggc gcgtccggcc agcgtcggca caatggccct gcgaccggcg
    44101 accgaggtct cggcgacgac ttcgcagtcg aagcgagaat cgaggatcga gcggccgacg
    44161 aacggctcac cgatgcggag ttcgccccgc gcatgcagca cggccatgcg cgccgagcag
    44221 ccggttccgc agggcgagcg gtcgagcttg ccgggctcga tcgcaaccgt gttccgcccg
    44281 gtcttgacgc cgtcgacaag ctccacgggt tgcgtcatct ggcagaacga gatgtgcgac
    44341 cagcccgggt tcgtcggatg cgtgaagccg agttgcgcgt tggctgccgc ggtgatcttc
    44401 acgccggtct cggcgagatc tcgggcttca tcgggcgtca gtgcaaagcc gagcaacttg
    44461 gcatcgacga gcacgaagct gtcgccgccg taggccgtgt cgaccgtcag tgtgccgagc
    44521 ccagcaacct cgatccgggc ccctagcttg tcggcaaagc tcgccacatt gcggatgcgc
    44581 acgcgttcga ccttgccgtc ccggcaggtt gcaatcgcct cgacgatccc gcccggcgcc
    44641 tcgagcatca cgcgcgtctc gggctcggcc atgggcacga tgccggtctc gagcagcacc
    44701 gtcgtcacgc agatgctgtt ggagccggac atcggcggcg tgtggatcgg ctccatgatg
    44761 atccaacccg tatgtgcctc cggcgacttc ggcggtacca gcagattgat atggcggaag
    44821 atgccgccgc gcggctcatt gaggaccaga ttgcgaaggc gctggtcgcc ggcgatgaag
    44881 cgggactgct cccagatcgt cgcgcctggc ggcgtcggca cgccaccgac gatgacgtcg
    44941 ccgacctcgc cctcacagtg gcagccgacg atatgaatga tcctgttcga gcgcatgccc
    45001 ccctccgcct cgaaactcaa gatcgagggc gccgccctcg agctcccgcc aagggctggc
    45061 ccttggaacc agttcagacc tgcacctgcg gccactcaat gccgctcatg gttcaggccg
    45121 gatcgtccgg ccaaagtacg ttcaatgcga aagcattgct cgacgaaggg tggagtcgca
    45181 accatgtcgg catctttgaa ggtcgccgcg atcagccatc tgctgctggc aagctcgaag
    45241 acctcaagag tgcgcgcatc gggatcgacg taccaggcat ggcggaccct gaacgcggcg
    45301 tagattgcga gcttgtccgt ccggtcgatg cgcatggtcg atggtgaaag cacctcgcac
    45361 agccaatcgg gcggcgtctc gatccaggcc gtatcgggga gccgcggcag ccgctcgcgc
    45421 cgccagccgg cgatgtccgg cacgacg
//
LOCUS       DAORNH010000006        43372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_665_length_43372_cov_2.650645, whole genome shotgun sequence.
ACCESSION   DAORNH010000006 DAORNH010000000
VERSION     DAORNH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 43372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43372
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_665_length_43372_cov_2.650645"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>410
                     /locus_tag="PK264_01120"
     CDS             <1..>410
                     /locus_tag="PK264_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRD74530.1"
                     /translation="ILILRELTGGTYFGEPKEIVTLEDGQKRAVDTTVYTTREIERIA
                     RVAFDLARKRRNKVHSAEKRNVMKTGVLWNQVVTTTHQAYAKDVELHHILADACAMQL
                     VRNPKQFDVLVCDNLFGDMLSDEAAMLAGSLGML"
     assembly_gap    411..455
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <456..723
                     /locus_tag="PK264_01125"
     CDS             <456..723
                     /locus_tag="PK264_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRD74531.1"
                     /translation="ALYEPIHGSAPDIAGQGVANPIAQIASFGMALRYSCNMAREADM
                     IDKAIAATLAQGYRTGDIMQPNMRKVGTTEMGRAIEGELKRLMG"
     gene            785..1369
                     /locus_tag="PK264_01130"
     CDS             785..1369
                     /locus_tag="PK264_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD74532.1"
                     /translation="MTLHTPPQSLGPAGRPRRLWTVGDVERMLAAGIVSERDRLELIG
                     GELIAIAPKGARHEIVRNELVLFWARRLPTDLKFAEEAPLRLGDYDMPEPDLILFPAS
                     LRVPDVRAETVLLVVEVSDSSLSYDIEVKAPRYAAAGVREYWVIDPTTLVTYVHRDPG
                     PDGYASKEERPGSARLEPHLVPALAVSLASLDVD"
     gene            1518..2213
                     /locus_tag="PK264_01135"
     CDS             1518..2213
                     /locus_tag="PK264_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD74533.1"
                     /translation="MISNVRAVFLALIAVFGLATPVSAAPNDIVVIITNQDYAGTVPK
                     VEYAERDGEAIAKAARDVMQVPPDRIVHVKNLTYAGFLDWFGADGEGGAELERLKAKL
                     GRDSNIVFYYSGHGMPAKRSKASDPEPVLLPVDTSPARADRFGMPTSVVRKALLTMQR
                     EKASDGRVILILDACFSGSSGGGRRDECCANFPVRRQGRRAAPRRTCGIRPRTGGQLG
                     PRAPAWHIHRWRS"
     gene            2668..3903
                     /locus_tag="PK264_01140"
     CDS             2668..3903
                     /locus_tag="PK264_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRD74534.1"
                     /translation="MKAKAGDLLASLDRSASDKERKAKEAAKLKAREEAEKKAKEETG
                     TAPTGPSDPATSAYCDRLKTYLAARAAAKDASAVRYVTGQSLCPDLQDEAKAILRTLD
                     DAGGTPAPTPLPADADPARGIEPGSGRGFVDHLRDGSACSHCPELVVVPADRFMIGSP
                     SSEAYRSPDEGPTREIAIARPFAIGRMEVTRGQFRAFVDSTGHQPAQNCSIWDGQNWK
                     IDQTMTWLSPGYEQDEDHPVVCVSHADAVKYAEWLSEQTGHVYQLPSEAEWEYAARAG
                     TSERYPFGASDGQICRFANVADSAAKKVNPSWTTVRCSDGFANTAPVGHFEASAWGLH
                     DMIGNVSEWTDDCATGSYDGYPGDGSPAKTGDCERRPHRGAGWSSTSEEARVARRSFY
                     FGEERFPTVGFRLVRVIAD"
     gene            3934..4398
                     /locus_tag="PK264_01145"
     CDS             3934..4398
                     /locus_tag="PK264_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD74535.1"
                     /translation="MAARPASVMTGGCQCGAVRYALYTAPTHASICHCRMCQKASGNY
                     FQPFTGVPRAEFTWTKGTPGTFRSSELVERDFCRDCGTPLTYRALDRDRVSVTIGSLD
                     EPGRIALEMQYGVESAVLDLGAAAQLTTITTEASMPAERLAQVATRQHPDHD"
     gene            complement(4404..5042)
                     /locus_tag="PK264_01150"
     CDS             complement(4404..5042)
                     /locus_tag="PK264_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRD74536.1"
                     /translation="MTNPFDCVVFDVGNVLIRWAPQNLYRKLGYSDEATASVLAETKL
                     LEVNHRELDAGAPFGPTLERLARRFPQHAAFISAFDRRWMDMLGGAITPNVAILAALR
                     RAGIPVHAITNFSREKFDETRRVFAFLDTFDECVVSGDLGLVKPEPEIFEHLVLKRGL
                     APARAVFIDDSAANIATAVGLGFKAVHYAEATVDLAHELQHLGMPSSTLGRA"
     gene            complement(5187..5648)
                     /locus_tag="PK264_01155"
                     /pseudo
     CDS             complement(5187..5648)
                     /locus_tag="PK264_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
     gene            complement(6038..6955)
                     /locus_tag="PK264_01160"
     CDS             complement(6038..6955)
                     /locus_tag="PK264_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD74537.1"
                     /translation="MSLYPPPQSIEARVFTRMPDNLRKRVPTVWADYNKPGQVVDCFL
                     EGPSFDQAGNLYVTDIPHGRIFRISAAGQWTQVTEYDGWPNGLKFHKDGRAFITDYKR
                     GIMALDVASGRVTPVVETIRSEGFKGVNDLHFAANGDLYFTDQGQTGLHDPTGRVYRL
                     GTDNRLACLIDTIPSPNGIVLDLHEKVVFVGVTRGNAVWRLPLMPDGQVSKCGLFIQL
                     SGGLGGPDGLALDAEGGIIVTHIGMGVWRFDRLGRPTHHIEPVGGRFVTNVAFGGVNG
                     QRLFMTESEGGSILTADLPVRGKAMYGPD"
     gene            complement(6995..8476)
                     /locus_tag="PK264_01165"
     CDS             complement(6995..8476)
                     /locus_tag="PK264_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD74538.1"
                     /translation="MWNAFVQAMGLVMDPYVLGVCLASAIFGIFVGAMPGLTATMATA
                     LLVPVTFYMSPVPALGAIVTATTMAIFAGDIPAAMLRIPGTPASAAYTDESYQMSQKG
                     QLNLNMGVNLVFSALGGLFGVAILIVCAPWLAELAINFSSFEYFWLAALGLTCAIFIA
                     TDDPLKGFAALFIGLSIGCIGQDPAAGHPRFTFGSVELLQGVSFIPAMIGMFAISELL
                     RGAVTVDQVGRVVAQQVGSIFFGVGTVFRRYWLNFIRGSFIGTAIGALPGAGADIAAW
                     ISYAVSKKFSKEPEKFGTGHIEGIVDSTSANNSALGGAWIPALVFGIPGDSITAIVIG
                     VLYMKNMNPGPTVFLQNPQLIYAVFIIFILANLLMLPVGWAAIKSAKLILRTPKNILV
                     PIILMFCVVGSFAMTNSVYGIIIMLILGVIGWLLEENGFPVAPIILGLVLGEMFEMNF
                     MTSMIKSDGSLFGFVSRPIAAVLGAMTIALWAFMLIRMRPKRA"
     gene            complement(8486..>8967)
                     /locus_tag="PK264_01170"
     CDS             complement(8486..>8967)
                     /locus_tag="PK264_01170"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRD74539.1"
                     /translation="NDTVNGLILIIGAIVMIALTLSFPPFPGQKYGPSLFPRILGGAL
                     ILCGILLINRGVREGRPAIALAPWAHDPWRVVSFMLIIAAALIAIFSWSTVGFVPVTV
                     VTLAGLFLWFRVHPVTALLTAILATVLLQIFFGRQMRIPLPLGWLLNLPPGWLKYIT"
     assembly_gap    8968..8990
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9031..10041)
                     /locus_tag="PK264_01175"
     CDS             complement(9031..10041)
                     /locus_tag="PK264_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD74540.1"
                     /translation="MTSRKTTFSRRRVLAGGAGLGAAAGLGVMPSLGQGAYPNRPVTF
                     VCPWGAGGGTDATARIMATLLEKDLKQPFNVVNRTGGSGVVGHSAIATAPADGYTIGM
                     VTVEITMMHHQGLTDLKHSSYTPLALVNEDPPGVQVNASSPYKTAKELADAIRAAPPG
                     KLKASGTGQGGIWHLALIGWLMAMKLKPDHVAWVPSNGAGPAMQDLAAGGIDIVTCSV
                     PEAKAMIDAGKARSLAVMASERNPQFKDVPTLKEAAGVDYSVGAWRGIAAPKGLPPEI
                     AKSLTEAIKRAWNSKEFVEFMSGRGFGLKWADPAGFADFMAKGDAAMGTAMTAAGLAK
                     KA"
     gene            10216..11622
                     /gene="rimO"
                     /locus_tag="PK264_01180"
     CDS             10216..11622
                     /gene="rimO"
                     /locus_tag="PK264_01180"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRD74541.1"
                     /translation="MSKPPSPRRAAVKTARQPASAKPVAKARRAAAPAPKVGFVSLGC
                     PKALVDSERIITKLRAEGYEVARDYAGADVVVVNTCGFLDSAKQESLDAIGEAMAANG
                     RVIVTGCLGVEKERILAAHPDVLAVTGPHQYEAVVGAVHDAVPPVHDPYADLVPPEGL
                     RLTPRHYAYLKISEGCNNRCSFCIIPSLRGRLASRPANHVMAEAESLVRAGVKELLVI
                     SQDTSAYGLDIKYAESPWKGRPLAARFFDLTETLGSLGVWVRLHYVYPYPHVDRVLPL
                     MAAGKILPYLDIPFQHASPKVLKAMRRPAAGEETLGRLARWRREVPDLAVRSTFIVGF
                     PGETEEDFTFLLDWLKEARLARVGCFKYEAVSGAAANELPGAVPEEVKEQRWHRFMAA
                     QQEISAAIMKERVGRTIDVLIDEVDEEGAIGRSPWDAPEIDGCVFVDAATGLKPGDKV
                     RVAITDADEYDLWGRIVS"
     gene            complement(11636..>12542)
                     /locus_tag="PK264_01185"
     CDS             complement(11636..>12542)
                     /locus_tag="PK264_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRD74542.1"
                     /translation="ARVAFSRDWATDAARRDFTINAIYCDGDGTIHDLVGGVADCARR
                     RIRFIGEARVRIREDYLRILRFFRFHAQLGGGAMEADGLAASIAERDGLQRLSMERIA
                     GELRKLLVGPNVVSAIAAMMDCGLLPSILRAAPRPALLSKAAAVEAAAGVAASPTLRL
                     SALAVAVAEDVDRLADHLKLAGCEREALLPIRSGIDVTPTLTDAERRRSLYRLGAQAW
                     RRHVFAAWIADGRIDEPSWLELLGLPERWPVPKLPVDGQSLIAAGLSPGPSFGAILKD
                     LETAWIASDFTLDRAALLKLVPPRR"
     assembly_gap    12543..12566
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12567..12848)
                     /locus_tag="PK264_01190"
     CDS             complement(<12567..12848)
                     /locus_tag="PK264_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRD74543.1"
                     /translation="MPTPRTLAGATWLAMPSTRAVLDALGRAGHEARIVGGAVRNTLM
                     GFPVTDIDIASTATPVEIVAAATAAGLKSVPTGIDHGTITVIAEGDPFEV"
     gene            complement(12887..13582)
                     /locus_tag="PK264_01195"
     CDS             complement(12887..13582)
                     /locus_tag="PK264_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRD74544.1"
                     /translation="MQETDDKSAAAITLTEAILRAIAARAFHAAPSDDIFDPRTGRSW
                     GRGDWDLNPEMLADFSVMPPPRPAAVLVPIVARDDGLTLLLTQRTEQLSTHAGQIAFP
                     GGKVEESDASPVAAALREAEEEIGLAASQVEPLGFLDSYRTGTGFLISPVVAIIRPGF
                     TLTPDASEVADVFEVPLGFLLDPRNHQRHSREWRGRERHYFAMPYGERYIWGATAGMI
                     RNLHDRLRESAAE"
     gene            complement(13590..14417)
                     /locus_tag="PK264_01200"
     CDS             complement(13590..14417)
                     /locus_tag="PK264_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD74545.1"
                     /translation="MSQTLISASPTDDLLVKRDKGVLVLTMNRPQHRNALTPAMVAAL
                     GAELAAAELDPDIGAVLLTGAGGAFCAGGDVKAMAAVHASGKSPDPVTRMELQRQQQR
                     VTSLRLYEMGKPTIAAIPGPAAGAGLSLALACDMRIAADSAVMTTAFAKVGLSGDYGG
                     TYLLTQLVGTAKARELYLLADRIEMSEALRLGIVNSVVPAAELEAKAMALARRFADGP
                     RVAHRLIKDNLNRALKGTFADCLDNESANLAKARETEDHKEAAAAFAAKRAPIFKGR"
     gene            complement(14428..14691)
                     /locus_tag="PK264_01205"
     CDS             complement(14428..14691)
                     /locus_tag="PK264_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74546.1"
                     /translation="MSATIEPLIRDLVEWCAKAPRTYEEVMATWRTSCPRLTVWEDAV
                     DRGLLERREAGSEIVVAVTDAGRCFLDPQRLKSPTRPHAPPTW"
     gene            complement(14709..16154)
                     /locus_tag="PK264_01210"
     CDS             complement(14709..16154)
                     /locus_tag="PK264_01210"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD74547.1"
                     /translation="MDGETTGGERLRSLEAAVSRDLHLTRHPEKAWMPTRKGPGGAHL
                     YDVVIAGAGQGGLAVAFALQRMRIDNILVVDRAAYGREGIWTTYARMPTLRSPKEFTG
                     PDLGVASLTYQAWHEARFGREHWERLHRIPKEHWQEYLLWYRAVLALPVRNETTLVAI
                     TPDNACLRLGLETPCGTETVVTRKLVLATGQDGLGRWLLPDFVERLPQHLRATTSDDI
                     DFAALAGKTIAVLGAGASAADNAATALEAGAAKVMMFVRRPALQRVQPYLWLTFSGFL
                     HHMADMDDHWRWKFMRHILELRESMPQETYDRCRRHAGFSIHVGAGWEGAEAAGDHVQ
                     LRTAAGDVTADFLIVGAGIDIDPSARPELASFAGEIATWADRYDPRETAQSPRLARFP
                     YHAPDGSFLERCPGAAPFLARIHDFTIATTMSFGPFGCSINAMTISAPRLARGITQGL
                     FREDLELHWASLQSHRDTIFEPAPVDRDKIG"
     gene            complement(16164..17579)
                     /locus_tag="PK264_01215"
     CDS             complement(16164..17579)
                     /locus_tag="PK264_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRD74548.1"
                     /translation="MPVDTALSAATPALRPPASETGLVGWMRKNLFSSVGNSILTVVC
                     LFFLYRLVAGALDWAVFSAVFTGEDGSACRGEGKGACWPFVKAKFSQFIYGRYPEEMR
                     WRVSLTFILALAGIIPLMIPAIPWKGFQAYYTLIVFPIIAYWLLTGVTVPVELPAAEL
                     ADIRSKQSWWYRIYSDARPYIGLSLLSAAAVSVLLSFVARQILIPQTMRLLGGILFTM
                     ALPYLIEMLFVALDQGKILGLAATNPPPGPHNALPVVKTERWGGLLVTLVVASVGITC
                     SYPVGIILALGRRSQMPIARWASVFFIETVRAVPLITILFMSSVMLPYFLPPGMEFDK
                     LLRALVGVALFAGAYMAETIRGGLQAIPRGQFEAADALGLSYPKKMGLIVLPQALKLV
                     IPGIVNSFVGLFKDTSLVLIIGLFDLLNIVQFNLTDAKWFAPQTAMTGYVFAAAFFWF
                     FCFAISRYSQWLEKRLRTDHK"
     gene            complement(17586..18848)
                     /locus_tag="PK264_01220"
     CDS             complement(17586..18848)
                     /locus_tag="PK264_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698297.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD74549.1"
                     /translation="MAVMDDGRPPAARRGASDDGLMSIFFRRETRQIIYQVAIVVTLV
                     AFFWMIISNAAARRQGRAWNAEFLNSSAGFDISQTLIPYSAASSNWDAFMVGILNTIF
                     VSAIAIVLATILGFVAGIARLSSNWLIAKVAAIYVEITRNIPLLLHLLIWYFGVIKNL
                     PGIFKQSPTGDFLMVDGKRVPGSVELGGGILLNNRGLFLPKPEFLPGSRTALFALVFG
                     IIASILVAMWARRRQLATGQQFPVLWTSLALILILPIGTFLVMGRPVQFEHPTVGRFN
                     MSGGIELLPELAALLIGLVFYTGGYIAEIVRAGILGVSKGQKEAARAIGLTNGQALRL
                     VVIPQALRIIIPPLTSNYLNVTKNSSLGIAIGYPDLVGVFTGTVLNQKSGHEIEIVLM
                     TMAVYLTLSLLTSLFMNWFNKRMALVER"
     gene            complement(19010..20047)
                     /locus_tag="PK264_01225"
     CDS             complement(19010..20047)
                     /locus_tag="PK264_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD74550.1"
                     /translation="MKRSFKIGAVAAAAAIAFTGLASAQTKPTLEVVKARGAVACGVG
                     PGTPGFSNPDDKGNWTGFDVDFCRAVAAAIFNDPKKVAFKPLTAKERFTALQSGEVDL
                     LSRTTTWTAARDSAMNLAFVGVMYYDGQGFMVNKKLGVKSAKELNGAAVCVATGTTTE
                     LNLSDYFRSMNMTYKPVVFEKSDEINAAYQAGRCDVYTTDASSLYAQRLRMQKPEDHV
                     ILPEIISKEPLGPLVRQADWQWFTLNKWVYFALLNAEELGVTSANVDKMLADTKNPEI
                     MRLLGRDKETDFGKPLGLTQDWVVRIIKGVGNYEEIYERNVGPKTPLKIARGVNNLWT
                     KGGLQYAPPIR"
     gene            complement(20269..20880)
                     /locus_tag="PK264_01230"
     CDS             complement(20269..20880)
                     /locus_tag="PK264_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74551.1"
                     /translation="MRLALVTAMLFGAVITSSPAANADCPTAVTASRGFKLEAHDPRM
                     ADHDIQPFDSETVRIFTSNPRNPSFVSEELTTWRGILQQKLSGRVAFELELDGDFRSL
                     HPVKGGATLATSYTVKVGDKVRKAKLKVRVDNEPTELTIGACTYQVWKIIRHSEFEDG
                     ERSVFHDYYAPDLSMWLRREVAVARPGQDLVLLIFAFKSITGR"
     gene            complement(20936..21976)
                     /locus_tag="PK264_01235"
     CDS             complement(20936..21976)
                     /locus_tag="PK264_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD74552.1"
                     /translation="MSGADRQLHYTHTRHPMGAETIELAPGLLWARFPLPFRLNHINV
                     WLLREDDGWTLIDTATTTREALDLWEALLSGPLSGAPVRRLIATHGHTDHVGLAGWLV
                     DRVGGVPFVATLAEWHSPQVRIIDAKQPMRPDTMRFLESHGCDQPTVAAYAQDRAKTH
                     ALLGPLPASFLRITDGETIRFGGRAWRVMVCGGHASEHASFYCEEERILIAGDQVLSK
                     ISPMIGVFPQEPWSDPLTEYLASLDRFRVLPEDTLVLPSHGMPFRGLHVRVDQLADHH
                     EARLDTLVGLMGRPEVAMTLARGLFPKAVDEGQGRHALAETLAHIHRLVTTGRARRNE
                     SGGKLLFERTDR"
     gene            22184..22906
                     /locus_tag="PK264_01240"
     CDS             22184..22906
                     /locus_tag="PK264_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD74553.1"
                     /translation="MQLTSVQAAPQLIDQVYDRLLSAIVDGTLAPGERLTQESVAALL
                     GVSRQPVSHALQVLRRRGLVVETGKRGLSVAPIDGARIRDLYQVREALDAIAARLAAE
                     RVVTGALSTEERREAEDCLACGARLADGCPTAELIAADVAFHSTLYRLSGNGAIAETV
                     AEQWPHFMRSMGAVLGEGGAVVRVWAEHAGILEAVLAGIPHLAAERARAHSARAGEAT
                     GRRLEALSQSADAAVVRRSIPA"
     gene            22935..23735
                     /locus_tag="PK264_01245"
     CDS             22935..23735
                     /locus_tag="PK264_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD74554.1"
                     /translation="MKLTTKQLADFERDGYIIIENCFSDAEMAVLRAEAEGIYMTNRE
                     EVWREKSGAPRTAFAAHKYNEAFGILGRHPRLIEPLEQFFGESVYMHQFKINAKAPFD
                     GEVWQWHQDYGTWKRDDGMPEARAMNISVFLDEVYPYNGPLMFIPKSHKGGTLAAGHD
                     LSTTSYPLWTLDNEQVTKLVAEGGIVAPTGKPGTVLMFHGNLVHASPPNITPYPRKIV
                     YLTLCAVSNHITQFKRPEFIAHRDFTPIKACADDALSVYVRKYRQAAE"
     gene            23924..25810
                     /locus_tag="PK264_01250"
     CDS             23924..25810
                     /locus_tag="PK264_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD74555.1"
                     /translation="MSRLIFRLAVCIALLIAVAAQASAEKRVALVIGNGAYVHAGRLP
                     NPPNDAADIAEKLKQLKFEVVVGRDLDNAGMRRIIRDFRVRAEGVDVALLFYAGHGMQ
                     IGGGNVLLPIDAKVDDELDVEPQTIRLDLVLAEMERAARVNVVLLDACRDNPLAERLM
                     RNIGARSRSLGLSRGLAKVDARGSDTLIMFATAPGDVAADGRGRNSPFTVALLKHLAK
                     PEELSVVLKDVVADVRAATGGQQRPQQLAAMERKLYLAGLPASSSGDSGETAKLKAEI
                     DRLRLERERMERERTEAERKSKLAAVAPSGPAVGAARLDRDIALKIQSILPLFLPVLG
                     TQPERFAAALSRSASAAGASAKVEVAPPNSVVPFSEILSALNSGRLDAAWGAMPILIG
                     RDKSFEIFSGQVPFGHDPARFVRWIESGGRPLLNGHARALRLHVVPCAIVGQEGGGWF
                     KKAIQRPEDLRGLRVRWYGSGNSVMQRVGALAQSIGAGNVHSMLTKGELDGTEFSLPS
                     VDATIGFHTIARHYYFPGWHAPALLSDLTFSRTAWDKLGPATQRVIEETCRHFLALTL
                     SEIAKDEAEGLAEIRAKPGVEIRPYPPAVLAVLRKAANEIQAETSANDAGYKAILDSY
                     RAAR"
     assembly_gap    25962..25971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25972..26249
                     /gene="hspQ"
                     /locus_tag="PK264_01255"
     CDS             <25972..26249
                     /gene="hspQ"
                     /locus_tag="PK264_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HRD74556.1"
                     /translation="IYPFRGIIFDVDPVFASTEEWWQSIPPDIRPKKDQPFYHLLAEN
                     AETEYVAYVSEQNLLPDTSGKPLRHPRVKELFAREGGRYKERFLQRH"
     gene            complement(26285..27517)
                     /locus_tag="PK264_01260"
     CDS             complement(26285..27517)
                     /locus_tag="PK264_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD74557.1"
                     /translation="MIATDAAPDRYALARAITCLGITQIIAWGTTYYCLGVLSTPIGR
                     EMGWSASTVFTGFAVALLSGSAASTTTGFLIDTYGARAVMTAGSVAVSIGLYALSGVV
                     DLMTYLITWAYLGIAMRLTLYDAAFAAIVQVAPDRGRRGISYLTLWGGFASSVFWPIG
                     HYLNGLYGWRQTLAIFAVFNLVACVPLHWFGITRHDKGSDTGQAKPAAAGAASAVDGR
                     PLEGRARSVGIALFALVMSFIAFVFSVTSVQLVHLLEQAGLATAAAVWLGSLKGVAQV
                     GGRILELTYGSRYHPMTIARIAIASLAASVVLLIMSGGSLWLAVAFTVLTGAAQGVLT
                     IVRGAVPLALFGASGYASVLGLIATPILVVSAASPIIYPWIVARIGPTGAGLLLLASS
                     AMAVILMELMSRWYDRQA"
     gene            27662..29035
                     /locus_tag="PK264_01265"
     CDS             27662..29035
                     /locus_tag="PK264_01265"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HRD74558.1"
                     /translation="MADDEANRFSARAARYVRVGTNVGAVAARVAGARLFGMDLDRQK
                     NAAELAKALGGLKGPIMKAAQLLSTIPEALPPEYARELAQLQSQAPPMGPAFVRRRMQ
                     AELGPDWQAKVARFEAEPAASASLGQVHRATSHDGRSLAIKLQYPDMQSAVEADVTQL
                     KVVFAIHARMDPAVDTADILQEIAARLREELDYTLEARHIALYAAIFKNDPRVRVPEV
                     VPELSTKRLLAMTWLEGRRLLDFKASPVEDRNEIARAMFRAWWFPFSHYGVIHGDPHL
                     GNYTIFSGDDGRPRGVNLLDYGCMRTFPPRFVQGVIDLYNGILRNDRALIVHAYETWG
                     FRGLSNELIDVLNIWARFIYGPLLDDRVRAIAEGTTAAEYGRKEAFTVHQALKEKGPV
                     KVPREFVFMDRAAIGLGGVFLHLDARLNYYQIFNETIDGFTLDAVTARQAAAFAAAGV
                     PLPVASF"
     gene            complement(29083..29328)
                     /locus_tag="PK264_01270"
     CDS             complement(29083..29328)
                     /locus_tag="PK264_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74559.1"
                     /translation="MAEQVIEVGFLTIIALYTLAGAARFSAAVDDLLYCNRELIWLVV
                     RRAASALGNWFGNIVMQATNYVAVRMVAGLLLACCWP"
     gene            complement(29449..29775)
                     /locus_tag="PK264_01275"
     CDS             complement(29449..29775)
                     /locus_tag="PK264_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74560.1"
                     /translation="MSARSAVALPAAADLSSSGRIARSDLEKLNKAKVALLHKLDPKK
                     DGDFQLAFVILNGTGIPHKDLSLEMGISPATFGRWVRGAHLPPTTEIREAYLRLAIKA
                     ITKLLP"
     gene            30048..30752
                     /locus_tag="PK264_01280"
     CDS             30048..30752
                     /locus_tag="PK264_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74561.1"
                     /translation="MRCDMAYRSTIVPRWIDDEERARDAHWYPAGCAIRKLTGRKIAH
                     LAMEIWTVDSSLPGAAPFRDYSSSAYSWRCDSGRQADCFADSRHHLYRGKWARLAGQC
                     IRELMLALRALYAAGQPSRPVLAPGFVGMPRVFSENLALSFQTPMNQSALDADAELAA
                     LSMLSSTSAGYLGTGLHELVPTKMNPWALDVLERFFGPSLTGGTIGLQYGDHMQKTSK
                     LVCNWPKLGEARALLK"
     gene            31064..32332
                     /locus_tag="PK264_01285"
     CDS             31064..32332
                     /locus_tag="PK264_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HRD74562.1"
                     /translation="MLALQSLIGIVGIPLLAWLASANRAAWPPRRLAMLVAAGLGAQL
                     LIALVLLKVPQAAGIFDVVTRLVRALQRATEAGVQMVFGHLAGGPPPYSVAAPQNTFV
                     LALNALPLILVLSALTRVLYHWGVLQRVVGGFAAVFQRTFGIGGPLATVAAAKIFLGM
                     VEAPLLVRPYLASMGRGALFAAMTLGMATIAGTVMALYALILDPVLPRAAGHVLAASL
                     MNAPAALMLAAIVVPDGFSDGPKSAEIRLENAPRSTMDAVAQGTVDGLKLLAAVVAML
                     VVMVALVSLVNELLSLFGTPGGRPLTLQRLLGYLCAPIAFAIGIPWGEAAAAGSLIGQ
                     KIVLNEFLAFLDLARTPQGVLSERSRLILTYGLCGFANLGSLGIMIGGLTAMVPERRD
                     DIIALAPRAMILGALATLLSGAIVALVGAG"
     gene            32338..33171
                     /locus_tag="PK264_01290"
     CDS             32338..33171
                     /locus_tag="PK264_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRD74563.1"
                     /translation="MSQAFAEIGGYALALAIGAAGALIFVALGFPLPWFLGALTACLV
                     ASVLGAPIKRPKPLSDPMRAVLGVAVGSAFTPALLGRAGGMVGSLLMLVPFMLVIILF
                     SRWFYERFAGFDRPTAFFSAIPGGLTDMTTMAEEAGANQRAVILVQASRILLIVFTLP
                     LWLKFHDGLAASSAFAGRARLHEIGFVDAGVLLALGFAGVVVARRIGLAGAPIVGPMI
                     LSGLAHALGLTSARMPVEVLTIAQVSVGVLLGCQFRGLTWAEFRSTMVWGLANTAHSS
                     S"
     gene            33135..33395
                     /locus_tag="PK264_01295"
     CDS             33135..33395
                     /locus_tag="PK264_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HRD74564.1"
                     /translation="MGSREHGALLVLTAGVVILVARLTGFDQVSVLLAFAPGGQTELN
                     LLTYVLGLDVAYVALHHLARLAIVILGAQMVFRAQSDWRKNG"
     gene            33411..34403
                     /locus_tag="PK264_01300"
     CDS             33411..34403
                     /locus_tag="PK264_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRD74565.1"
                     /translation="MKALVYTGPEQMVYRDEPDPQANDDVIVRVGACGICGSDMHAYH
                     GHDARRPPQLILGHEAAGIATNGAFKGRRVAINPLVPCGSCDECLDGRPHVCGTRQIL
                     SMPPRPGALAELVRVPERNLVLVPDGLATEVAALAEPMAVGYHAVGLAERALPRPLAA
                     ARIAVIGGGAIGLGTAQILECRGGRGITIAETSEIRRIAMKKAGRFQPYDPRGSGAPG
                     ANSMHLVIDAFGGKSSREEACRIVKPGGVIIHIGLASGEGGVDVRKLTLQEVSFIGSY
                     CYTMVDFRETLAGLSSGQFGPIDWTEERPLAEGVKAFAELNDGKVAAGKIVLRM"
     gene            34421..>35100
                     /locus_tag="PK264_01305"
     CDS             34421..>35100
                     /locus_tag="PK264_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD74566.1"
                     /translation="MIVVEKSAAATALVITRPTRRNALDQTTLLALREGIREAEADTA
                     CHCLVLRGAGGTFCSGRDLGDARRDAPLEELLAYDETWGDIIGGLRAMTKPSIAVVSG
                     HAVAGGFTLAMACDFVLAERGARFGALEMRGGFPAAVNTAVLTHLVSRRIALRYLLSD
                     ETFAAEELLAHGLVSEIADGGSALERLEAASVERLTRLDPTAVKLTKEANRSMAASPE
                     DVAIRSGSR"
     assembly_gap    35101..35124
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35293..36354
                     /locus_tag="PK264_01310"
     CDS             35293..36354
                     /locus_tag="PK264_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD74567.1"
                     /translation="MVKPILIVGGGPAGLAAAHALSSVGQMCALVEKSAALGGAPIQS
                     GYAKLVPSGLWAKDAIGGMVSRVEAASQYVQVLKGTTVKSFTGTAGNFVATMSDGATG
                     EFGAAILTTGFTHFDSVNKPEWGFGTFPDVVTTTQVEQMISSGKGVRCPSDGRKPKRV
                     AILLCVGSRDRQIGRDWCSKICCTVSANMAMEIREELPDCHVYVYYMDIRTFGHYETD
                     YYWRSQEEFKVKYIKARIAEVTSDGKQLIVKGEDTLVKRPITIPFDMVVHAIGMDPNV
                     DNMTLSAVFGVDLHKHGYIARKDTYGVMGATSRPGVFVAGAAIGPETIDDSIAQANAA
                     ALSALAAARGGARAMETVA"
     gene            36351..36914
                     /locus_tag="PK264_01315"
     CDS             36351..36914
                     /locus_tag="PK264_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74568.1"
                     /translation="MNRLFRKAVVVLLAATAMTVLPATAQRAKSYDVCLSPAEYLRLY
                     GAEAAPGACAAWPEPSPAELEMAICRAAGPDTWKLELKGSAAAGGPTSFELAAVRLRA
                     SLPITFSTELQGAGEVGLHIYTLIDLELGAEEIDPTRRSEVLAGEPRIERLTKIDGRC
                     EAAQLTPAATLLALPAWVEGPQSRRDK"
     gene            36911..37741
                     /locus_tag="PK264_01320"
     CDS             36911..37741
                     /locus_tag="PK264_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74569.1"
                     /translation="MISAALTSPDTGLLRPVLHLSGPKLRGSLEGLIKAAEELGGIES
                     FVAAVKLKSELFQERLGEGRAAAIERGAFDDLIRFMPTVRRRISALVATDAWSGTRAA
                     MAELLQDAHQPGTGDARIAAFEGALRERATATDGAHARFHRDFAAELLHNVWPEHYPL
                     MTRWVWDAKANTGVLREIWHDPIAGDATDQIVIDAPDTHDAFLVLREELSHFLSEQGI
                     FRDMLWFVDLVEAHIYGGYINSQGGAWLKADFSAEGDPLEHTRRILGLDARRRPSAGG
                     "
     gene            37820..38608
                     /locus_tag="PK264_01325"
     CDS             37820..38608
                     /locus_tag="PK264_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRD74570.1"
                     /translation="MPIHEKSLIRPENLKTHDKLVIDGVDVSGHWSTFITSRVVTDYN
                     ENLEDEIAAMPGGDKIHRCWQCGSCTNACTINAIEPRFNPRYWIYLIRMGYESELLRD
                     KDIIWQCVSCHKCTNICPRDVEPEGVMKATAHWLHMKGYTPPSPSHIFDEEFSRQVVE
                     RGKIEDTEVLHKFLHKTKQPLMQDWLVAIGTGIAKRLPFVWGIKMMLAQLFKPRTRNW
                     SKARAAIEDYIHEKEDERRAVIAGSRKAAPPAHAGKAGHMMAAE"
     gene            38613..38900
                     /locus_tag="PK264_01330"
     CDS             38613..38900
                     /locus_tag="PK264_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HRD74571.1"
                     /translation="MLFMVIERFKDADPKPVYRHLRDQGRGLPDGLKYVASWIEPNFD
                     RCFQVMECDDARLLMEWAMHWREHATFEIVPVVPSAETRALVEKSLDQEKR"
     gene            38902..39846
                     /locus_tag="PK264_01335"
     CDS             38902..39846
                     /locus_tag="PK264_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HRD74572.1"
                     /translation="MVDVTEKAKKYRSVAYYPGCALEGTGNGYDVSTRAIAKELGLGL
                     KDVKDWNCCGAMEVKNVDPKIQTYLSARVLSQAVHENQQDVVMAPCNGCYHNLKKAEH
                     DLAHDQASRDVTERLSSKAGHPTYKAGEAETIHALDWLKATIGEEGIKARTKGRLAGL
                     RIANYYGCMYVRPRHIFPEKDKGPGSESTHAPHFMDDLLAAAGATNVSFPLKTACCGG
                     AHTLSDSDTSTKLVLNILQAAEASGADVIATECPTCHSGLEMHQVRAEKRFGKKTNVK
                     MLYFTQLLGLALGIGPRKLGIHTNISDSRDLIIEKQLT"
     gene            complement(39854..>41138)
                     /locus_tag="PK264_01340"
     CDS             complement(39854..>41138)
                     /locus_tag="PK264_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD74573.1"
                     /translation="SAVPASLPGIVFDEPFAERRVGRANIVWVTGPELVEEVLIGGAE
                     RFHKTSVEKRVLSPALGKGLLTSDGPAWRWQRRTAAPLFRPQDLTALVPAMAAEGERI
                     VAAWAAKPGERTIAVEHAMGDLTFRIIASTMFSGDGLEVGDLIQNAGRRYLGPVTWEI
                     AAGQLRLPDGIWHPGRGRMRRAARDLRSIVAMVISRRRAEGGERRDLLGRLMSARDPE
                     TGEPMSEEQLIDNMVTFYMAGHETTAVALTWTLYLLARAPAWQELIRAEAAQVAGRAP
                     IEASHIDRLVVTRRVLKEAMRIYPPVAMMARTIDEPTTLGSHRLHAPTLVLIPIFAIH
                     RHNRLWEDPKRFDPDRFLPAAEARHARTQWMPFGYGPRVCIGATFAMLEATALLATLV
                     RGARFGWDGRHTPEPVSRVTLRPKGGMPLQVTPIS"
     assembly_gap    41139..41254
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(41334..41510)
                     /locus_tag="PK264_01345"
     CDS             complement(41334..41510)
                     /locus_tag="PK264_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74574.1"
                     /translation="MSKRSLRNVEKLKKKQRRTAARPAQSRSQGNTSGARTRLKKSAG
                     IARARKAKAAATAT"
     assembly_gap    41616..41625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            41734..42249
                     /locus_tag="PK264_01350"
     CDS             41734..42249
                     /locus_tag="PK264_01350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD74575.1"
                     /translation="MLGSDHKFRIRKGVPADAAALAVLFRETWELTYRAIIPAVHLET
                     LIRRRNEDAWRLALGASEGILVLEVLGVIAGYASFGAARGRGRSAYRGEIYELYLAPV
                     YQGLGLGEHLFEATRSALDQRRLSGLIVWALADNLPALSFYWARGGRPIARVTDRMAG
                     VPLPKVAYGWT"
     gene            complement(42262..>42995)
                     /locus_tag="PK264_01355"
     CDS             complement(42262..>42995)
                     /locus_tag="PK264_01355"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRD74576.1"
                     /translation="APPPANYRPPAAPSAPNPWLPRREDDDDRRSSRSGAYRTVCVRL
                     CDGFYWPLSYATSRSNFWKDASRCRASCGEDAQLFYLPNTAEPAEMVDLTGRAYTKLP
                     TAFKYRKTLVENCKCKPEPWAASEAARHKAYADAAEAEESRKRIATGHPGDASGDPDT
                     PAPTAAASTVDPKPADPPTSPWGVPTTTPAPAAKRPPRPRDIAAAPRRTSPVATPGSP
                     PGAPTWPGSSMGLGSKGMLWPGDSR"
     assembly_gap    42996..43005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7505 a        13893 c        14598 g         7114 t
ORIGIN      
        1 acatcctgat cctgcgcgag ctgaccggcg gcacctactt cggcgagccg aaagagatcg
       61 tgacgctcga ggacgggcag aagcgcgccg tcgatacgac ggtctacacg acgcgcgaga
      121 tcgagcgcat cgccagggtc gcattcgacc ttgccaggaa gcggcgcaac aaggtgcact
      181 cggccgagaa acgcaacgtc atgaagacgg gcgtgctctg gaaccaggta gtgacgacga
      241 cgcaccaggc ttatgccaag gacgtcgagc tgcatcacat tctcgccgac gcctgcgcca
      301 tgcagctcgt gcgcaacccg aagcagttcg atgtgctcgt ctgcgacaac ctgttcggag
      361 acatgctctc ggacgaggcc gcgatgctcg ccggctcgct cggcatgctg nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgc tctacgagcc gatccacggc
      481 tcggccccgg acatcgctgg ccagggcgtc gccaatccga tcgcccagat cgcctcgttc
      541 ggcatggcgc tgcgctactc gtgcaacatg gcgcgcgaag cggacatgat cgacaaggcg
      601 atcgcggcga cgctcgccca gggctaccgg accggcgaca tcatgcagcc caacatgcgc
      661 aaggtcggca ccaccgagat gggccgcgcc atcgagggcg agctcaagcg gctgatgggg
      721 tgagaggcga ggggcgattg tcggcgtgtt tcgtccgcgc tcgccttcgt gctagattcg
      781 tcgcatgacc ctccacacac caccccagtc cctcggacct gccggccggc cgcggcggct
      841 ctggaccgtc ggtgatgtcg agcgcatgct cgcggcgggc atcgtctcgg agcgcgaccg
      901 gctcgagctc atcggcgggg agctgatcgc aatagccccg aaaggcgcgc gccacgagat
      961 cgtcaggaac gagctggtgc ttttctgggc acgccgcttg ccgacagacc tcaagttcgc
     1021 cgaggaggcg ccgctgcggc tcggcgacta cgacatgccg gagccggacc tcattctgtt
     1081 cccggcgtcg ctcagagttc ccgacgtacg cgcggagacg gtcctgcttg tcgtcgaggt
     1141 ctcggattcg agcctctcct acgatatcga ggtgaaggcg ccccgctatg ccgccgcagg
     1201 cgtgcgcgaa tactgggtga tcgacccgac gacgctcgtc acctacgtcc atcgcgatcc
     1261 gggaccggac ggttatgcgt cgaaagaaga gcggccgggg agcgcacggc tcgagccgca
     1321 cctggtgccg gcactcgccg taagcctcgc ctcgctcgac gtcgattgac gcgacaccgg
     1381 ccaaggaatc cggctgagcc atcgtccgag gtgacgagcc ggccgcttca ccctgccggc
     1441 gcgcgcgggc tcgcgtgact gacggctcac ggtcggctaa catgctggcc aagcctgccg
     1501 tttttggagc agcccccatg atttccaacg tccgggccgt ttttctggcc ctcattgccg
     1561 tattcggcct tgcgactccc gtcagcgccg cgccgaacga catcgtcgtc atcatcacca
     1621 accaggacta cgccggcacc gtgccgaagg tggagtacgc cgagcgtgac ggcgaggcga
     1681 tcgccaaagc cgcccgcgac gtcatgcagg ttccgcccga tcgcatcgtt catgtgaaaa
     1741 atctcaccta cgcgggcttc ctcgactggt tcggcgccga cggcgagggc ggcgcggagc
     1801 tcgagcggct caaggccaag ctcggccgcg acagcaacat cgtattctac tattccggtc
     1861 acggcatgcc ggcgaagcgc tcgaaggcga gcgacccgga gcccgtgctg ctgccggtcg
     1921 atacctcgcc ggcgcgcgcc gatcgcttcg gcatgccgac gtcagtggtg cgcaaggccc
     1981 tgctcacgat gcagcgcgag aaggcgtccg acggccgcgt catcctgatc ctcgacgcct
     2041 gcttctccgg ctcgagtggt ggtggacggc gagacgagtg ctgtgcgaat ttccccgtcc
     2101 gccgtcaggg ccgccgagcc gcgcctcgtc gaacttgcgg catccggccc cgaacaggag
     2161 gccaactggg accgcgagcg ccggcatggc atattcaccg atggcgttct tgagggcctc
     2221 tacggggcgg ccgacgccaa caaggacggt tcggtgacgg ccggcgagct cgcgacattc
     2281 gtcgcgcgct acgtcgatga ccgcgtgacg cgcctgttcc cttcgccgcg ccgcttccag
     2341 acgccgtcgt tctcgggtga ggcgccgacg cggctttcga tcgtcgcggc cacgacaccc
     2401 gcccgcgacc cgaaggccaa ggcgctgatc gagacacgtt gcaaaaccgt gcccgcgtcg
     2461 acggataccg ccgagataaa gtcgttcctc gcgacttgcg gcttcaattg tccgtgccgg
     2521 gcagcgctcg atgcgcgtct cgccgccctc gcagtgctct cggcaacgtg cgaggcggaa
     2581 gcgttcgagc tcgatcggct cgccaagcgc ggcgctgcag caagagccga gctcgaggcg
     2641 ctcgccaagg gcgcgtcgtg cccggccatg aaggccaagg ctggcgacct cctcgcctcg
     2701 ctagatcgca gcgccagcga caaggagcgc aaggcgaagg aggcggccaa gctgaaggcg
     2761 cgcgaggagg ctgagaagaa ggccaaggag gagaccggaa ccgcgcccac gggcccgtcg
     2821 gacccagcca cctctgccta ttgcgaccgc ctcaagacct atctcgccgc gcgcgcggcg
     2881 gccaaggacg cgagcgcggt acgctacgtt accggacaat cgctctgccc cgacctgcag
     2941 gacgaggcca aggccatcct gcgcacgctc gatgatgccg gcggaacgcc ggcacccacg
     3001 ccgctgccgg cagatgccga tcccgcgcgc ggcatcgagc cgggttccgg ccgcggcttc
     3061 gtcgatcatc tgcgcgatgg gagcgcatgc tcgcattgcc cggagctggt ggtcgtgccg
     3121 gccgatcgct tcatgatcgg atcgccgtcg agcgaggcct accgcagtcc ggatgaaggt
     3181 ccgacgcgcg agattgcgat tgcgcgcccg ttcgccatcg gacggatgga ggtgacacgc
     3241 gggcagttcc gcgcatttgt cgacagcacc ggtcatcagc cggcacagaa ctgcagcatc
     3301 tgggacgggc agaattggaa gatcgaccag acgatgacat ggctcagtcc ggggtacgaa
     3361 caggatgagg accatccggt cgtctgcgtc tcgcatgccg atgccgtaaa gtacgcagag
     3421 tggctcagcg agcagaccgg ccatgtctac cagctgccat ccgaggccga atgggagtat
     3481 gcggcgcgcg ccggcacctc cgagcgctat ccattcgggg cgagcgacgg ccagatctgc
     3541 cggttcgcca atgtcgcgga cagcgcggcc aagaaagtga accccagctg gacgacggtc
     3601 agatgctcgg acggctttgc gaacacggcg ccggtcgggc acttcgaagc cagcgcgtgg
     3661 ggtctccacg acatgatcgg caacgtcagc gaatggaccg atgattgcgc gacgggcagt
     3721 tacgacggct atccgggcga tggctcaccg gccaagaccg gcgactgtga gcggaggccg
     3781 catcgcgggg ctggctggtc gtcgacttcg gaggaggcga gggttgcgag gcgcagcttc
     3841 tacttcggcg aggagcggtt tccgaccgtc ggcttccggc tcgtccgcgt gattgcggat
     3901 tgaaaccgga ttgatcatgg ggaggatttg gatatggctg ctcgtcctgc cagcgtgatg
     3961 accggcggct gccagtgcgg cgcggtccgc tacgcgctat acaccgcgcc gacgcatgcc
     4021 tcgatctgcc actgccgcat gtgccagaag gcgtccggca actacttcca gccgttcacg
     4081 ggcgtgccgc gggccgagtt cacctggacg aaggggacgc ccggcacgtt ccgctcgtcc
     4141 gagctcgtcg agcgtgactt ctgccgcgac tgcgggacgc cgctcactta ccgcgcgctc
     4201 gaccgcgatc gtgtgtcggt gacgatcggc agcctggacg agccggggcg gattgcgctc
     4261 gaaatgcagt acggtgtcga gagcgcggtg ctcgatcttg gcgcggcggc acaactgacg
     4321 accatcacca cggaggcgtc gatgccggcc gagcggctcg cgcaggtcgc cacacggcag
     4381 caccctgacc acgattgatg ccgtcaagct cggccgagtg tcgatgacgg catgccgagg
     4441 tgttggagtt cgtgagcgag gtcaaccgtc gcctcggcat agtgcacggc cttgaagccg
     4501 aggcccaccg ctgttgcaat attggcggcg ctgtcatcga tgaatacggc gcgggcgggc
     4561 gcaaggccgc gcttgagcac gaggtgctcg aagatctcgg gctcgggttt gacgaggccg
     4621 agatcgcccg agacgacgca ttcgtcgaac gtgtcgagga acgcgaacac gcgccgcgtc
     4681 tcgtcaaact tctcgcggct gaaattggtg atcgcgtgga cgggtatccc ggcgcgcctc
     4741 agcgctgcga gaatggcgac attcggcgtg atcgcgccgc cgagcatgtc catccagcgc
     4801 cgatcgaagg cggaaatgaa tgccgcgtgc tgtgggaacc gtcgtgcgag ccgctcgagt
     4861 gtcggtccga acggcgcgcc tgcgtcgagc tctcgatgat tgacctcgag gagcttcgtt
     4921 tcggcgagca cgctggccgt cgcctcatcc gagtagccga gcttgcgata gagattctgc
     4981 ggggcccagc ggatgaggac attgccgaca tcgaagacga cgcagtcgaa cgggttcgtc
     5041 atggcgtcct ccgtgtgttc cgagacccga tcttgcagat ggcgccttca gcggcgaagc
     5101 ccgaatgggc gaccccacga cgagccgggc gtgaggctcg cactcgaagc ccacggctgc
     5161 cgtgcgagcc aggcatgctc gtttgcttcg ttcccgtcga gcggcgcagg gccggcgtag
     5221 cgtacgcgca ccggcgtcgt tccgttcgcc tcgaagccga ggagctgcgc gacccgccgc
     5281 gagagatcga tcagccgatc gctggcatag gggccgcggt cgttgacgcg aacgagcagc
     5341 gtgcgcccgt tgcggatatt ggtgacgtag acgtacgagg gcagaggcag ggtcggatgc
     5401 gcggcggtaa gcgcgcccat gtcgaagagt tcgccgtttg cggttttgcg gccatgaaaa
     5461 tcgcttccgt accaggaggc gatgccaact ctgtcgtagc ggggctcgtc ggccggcgtg
     5521 taccagcggc cggcgatctg atacggctgc ccgatcttgt agatgccccc gccgcgcggg
     5581 acacgcccca agggcgaggc ggccaagcgc gggctcggcg agacgccgta acgatcatcg
     5641 accgtcgatg gagtagcggc gcgaatcggg tgggcgtcgg ctgccggagc ggctggtggt
     5701 cggcgctggg cgcatcctcc gagtagtcct gcgacgatta ccgccgcaac tcccggaaca
     5761 agcactctgc gccggacgcg ctcacgcgcg tccccgtagg cgtcccccgc catcacccag
     5821 ccacgcacga caaaccgacg ccccactcta cccccaagcc tgatgcggca tcgcgattgg
     5881 tcggctggcg cgaatgagct cggataccgc gtttcgagca cggtccgact tctgctgtcg
     5941 cgctaagcct cgaggccgcg tcaggtccca catcgctaaa attgccacga taaaattaga
     6001 gccgcgttaa tggcggcgat ttttggcccg actgtgttca gtccggaccg tacatcgcct
     6061 tgcccctcac gggcagatca gccgtgagga tcgagccgcc ttctgattca gtcatgaaca
     6121 ggcgctgccc attcacgccg ccgaacgcca cgttggtgac aaagcgtccg ccgaccggct
     6181 cgatgtggtg cgtcggtcga ccgaggcggt cgaagcgcca cacacccatg ccgatgtgcg
     6241 tgacgatgat cccgccctcg gcatcgagcg cgagcccgtc cgggccgccg agtccgcccg
     6301 agagctggat gaagagcccg catttcgaca cctggccatc cggcatcagc ggcaagcgcc
     6361 agactgcatt gccgcgcgtc acgccgacga acacgacctt ctcgtgaagg tcgagcacga
     6421 tgccattggg gctcgggatc gtgtcgatga ggcaagcgag gcggttgtcc gtgccgaggc
     6481 gatagacgcg ccccgtcgga tcgtgcaggc cggtctggcc ctgatcggtg aagtagaggt
     6541 cgccgttcgc cgcgaagtgg agatcgttga cgcccttgaa gccttcggag cggatggtct
     6601 cgaccacagg cgttacgcga ccgctcgcga cgtcgagggc catgatgccg cgcttgtagt
     6661 cggtgatgaa ggcgcggccg tccttgtgga acttgaggcc gttcggccag ccgtcgtact
     6721 cggtgacctg ggtccattgg ccggccgcgg agatgcgaaa gatgcggccg tgggggatgt
     6781 cggtcacgta gagattgccg gcctggtcga agctcgggcc ctccaggaag caatcgacga
     6841 cctgtccggg cttgttgtag tcggcccaga cggtcggcac gcgcttgcgg aggttgtccg
     6901 gcatgcgcgt gaagacgcgc gcttcgatcg actggggcgg cgggtagagg ctcatggccg
     6961 gtaggtcttt cgggttggaa ggcgactgtt ccggctatgc ccgtttcggt cgcatcctga
     7021 tcagcatgaa tgcccagagc gcgatcgtca tggcgccgag cacggccgcg atcggccgcg
     7081 acacgaatcc gaacagcgag ccgtcggact tgatcatcga ggtcatgaag ttcatctcga
     7141 acatctcgcc gagcacgagg ccgaggatga tcggggcgac cgggaacccg ttctcctcga
     7201 gcagccagcc gatcacgccg agtatcagca tgatgatgat gccatagacc gagttggtca
     7261 tggcaaagct gccgacgaca cagaacatga ggatgatcgg caccaggatg tttttcggcg
     7321 tcctcagaat gagcttcgcg ctcttgatgg cggcccagcc gaccggcagc atcaggaggt
     7381 tcgccaggat gaagatgatg aacacggcgt agatgagctg agggttctgc aggaacacgg
     7441 tcggccccgg gttcatgttc ttcatgtaga ggacgccgat gacgattgcc gtgatcgaat
     7501 ctccgggaat gccgaaaacg agggccggga tccaggcgcc gcctagggct gaattgttcg
     7561 ccgacgtgct gtcgacgatg ccttcgatat gtccggtgcc gaacttttcg ggctccttgg
     7621 aaaacttctt cgagaccgcg tagctgatcc aggcggcgat atcagcgccc gcgccaggga
     7681 gcgctccgat cgcggtgccg atgaaggagc cgcgaatgaa gttgagccag tagcggcgga
     7741 agacagtgcc gacgccaaaa aagatgctcc cgacctgctg ggccaccact ctgccgacct
     7801 gatcgaccgt tacggcaccg cgcagaagct ccgatatggc gaacatgccg atcatggcgg
     7861 ggatgaagct gacgccctgc aagagttcca ccgatccgaa ggtgaagcgc gggtgtcctg
     7921 cggcagggtc ctgcccaatg cacccgatcg agaggccgat gaacagcgcc gcgaaaccct
     7981 tgagcggatc atccgtcgcg atgaagatcg cgcaggtgag gccgagagcg gcgagccaga
     8041 agtactcgaa cgacgagaag ttgatggcaa gctcggcaag ccacggcgcg cacacgatca
     8101 ggatcgccac cccgaacagg ccgccgagcg cggagaagac gaggttgacc cccatgttga
     8161 ggttcagctg ccccttctga gacatctgat agctttcgtc cgtataggcg gcggacgcgg
     8221 gcgttcccgg gatgcgcagc atggccgccg ggatgtcgcc cgcgaagatc gccatggtgg
     8281 tcgccgtaac gatggcgccg agggcgggca caggcgacat gtagaacgtg acgggaacca
     8341 gcagcgcggt ggccatggtc gccgtcaacc cgggcatcgc gccgacgaag atgccgaata
     8401 tggccgaggc caggcaaacc ccgagcacat aggggtccat gacgaggccc atggcctgga
     8461 cgaacgcgtt ccacatcggg gcgcctcaag tgatgtattt gagccagccc ggcggcagat
     8521 tgaggagcca gccgagcggg agcgggatac gcatctgcct gccgaagaag atctgcaaca
     8581 ggacggtcgc gaggatcgct gtcagcaagg cggtcaccgg gtggacgcgg aaccagagaa
     8641 agagccctgc aagagtgacg acggtgaccg gcacgaagcc gaccgtcgac caggaaaaga
     8701 tcgcaattag cgcggctgcg atgatgagca tgaaggagac gacgcgccac ggatcatgcg
     8761 cccagggcgc cagagctatc gccggacggc cctcgcgtac accgcgattg atgagcagta
     8821 tgccgcacag tatgagcgcg ccaccgagaa tgcgcgggaa aagtgatggt ccgtacttct
     8881 gcccgggaaa aggcgggaag gagagcgtca gcgcgatcat gacgatcgcg ccgatgatca
     8941 gaatgagacc gttgaccgtg tcgttggnnn nnnnnnnnnn nnnnnnnnnn aaaaggacgt
     9001 gcgccccgct gtatggcggg gcgctgatgc tcaggccttc ttggcgaggc cggctgccgt
     9061 catcgccgtg cccatggcag catcgccctt ggccatgaag tcggcaaagc ccgcgggatc
     9121 ggcccacttg aggccgaagc cgcggccgct catgaactcg acgaactcct tgctgttcca
     9181 ggcgcgcttg atcgcctcgg tgagcgactt ggcgatctca ggcggtagtc ccttgggcgc
     9241 cgcgatgccg cgccatgcgc cgaccgagta gtcgacaccc gcggcctcct tcagcgtcgg
     9301 gacgtccttg aactgcgggt tgcgctcgga ggccatcacc gcgagagagc gtgccttgcc
     9361 ggcgtcgatc atggccttcg cttcgggcac cgagcaggtc acgatgtcga tgccgccggc
     9421 cgcgaggtcc tgcatcgcgg ggccggcgcc gttcgagggc acccaggcaa cgtgatctgg
     9481 cttcaacttc atcgccatca gccaaccgat gagggcaaga tgccagatac caccctggcc
     9541 agtgccggag gccttgagct tgcccggggg tgccgcgcgg atggcatccg caagctcctt
     9601 tgccgtcttg tagggactcg aggcgttgac ctgcacgccc ggcgggtcct cgttgacgag
     9661 ggcgagcggg gtatagctcg agtgcttgag gtcggtcagg ccctgatggt gcatcatggt
     9721 gatctcgacc gtcaccatgc cgatcgtgta gccgtcggct ggcgccgtgg cgatcgcgga
     9781 atgcccgacc acgcccgagc cgcccgttcg attgacgacg ttgaacggct gcttcagatc
     9841 cttctcgagg agcgtcgcca tgatgcgcgc cgtcgcatcg gtgccgccgc cggcgcccca
     9901 ggggcagacg aaggtgacgg ggcggttcgg ataggcgccc tggccgagcg acggcatgac
     9961 gccgagccct gcggccgccc cgagcccggc accgcctgcg agcacgcgac gacgcgagaa
    10021 agttgtcttg cgactggtca ccggcatttc ctccaggatg attattgtcc agtcaggcta
    10081 tcgcgctgca cgcgacgctg gcaagccgca ccggcgacat tcatgccatg cgctgaccgt
    10141 acggggccag ggtcgcatga ggccatggtc actcaaacat tggcaaaccg gggcgcctct
    10201 gcttattagc ggcgcatgag caaaccaccc tccccccgcc gcgccgccgt caagaccgcg
    10261 cgccagccgg cgagcgcaaa acccgttgcg aaggcacgcc gggcggcggc gcctgcgccg
    10321 aaggtcgggt tcgtttcgct cggctgcccg aaagcgctcg tcgacagcga gcgcatcatc
    10381 accaagcttc gcgccgaggg ctacgaggtg gctcgcgact atgccggtgc ggatgtcgtc
    10441 gtggtcaaca cctgcggctt cctcgacagc gccaagcagg agagcctcga cgcgatcggc
    10501 gaagccatgg cggccaacgg ccgcgtcatt gtcaccggct gcctcggagt cgagaaggag
    10561 cgcattctcg cggcacatcc ggacgtgctc gccgtgacgg gaccgcatca gtacgaggcg
    10621 gtcgtcggcg cggtgcacga tgcggtgccg cccgtgcatg atccatatgc cgacctcgtc
    10681 ccgccggagg ggctccgcct gacgccgcgc cactacgcct acctgaagat ttccgaggga
    10741 tgcaacaacc gctgctcgtt ctgcatcatc ccgagcctca gggggcggct cgcgagccgc
    10801 ccggcaaacc acgtgatggc ggaggccgag agcctcgtgc gggcaggcgt gaaggagctt
    10861 ctcgtcatca gccaagatac gagtgcctat ggcctcgaca tcaagtatgc cgagagcccg
    10921 tggaaaggcc ggccgctcgc tgcccgtttt ttcgacctca ccgagacgct cggctcgctc
    10981 ggcgtatggg tccgcctcca ctacgtctac ccgtacccgc acgtcgatcg cgtgctgcca
    11041 cttatggcgg ccgggaagat cctcccctat ctcgacattc ctttccagca tgcctcgccg
    11101 aaggtcctga aggcgatgcg ccgccccgcg gccggcgagg agacactcgg ccggctcgcc
    11161 cgctggcgtc gcgaagtccc tgatctcgcc gtccgctcga ccttcatcgt gggtttcccg
    11221 ggcgagaccg aggaggattt cacgttcctg ctcgactggc ttaaggaggc gcgcctcgcc
    11281 cgcgtcggtt gcttcaagta cgaggcggtc tcgggtgctg cggcgaacga gcttcccggc
    11341 gccgttcccg aggaggtgaa ggagcagcgc tggcatcgct tcatggcggc gcagcaggag
    11401 atcagcgcgg ccatcatgaa ggagcgcgtc ggccgcacga tcgacgtgct gatcgacgag
    11461 gtggatgagg aaggcgccat cggccgctcg ccctgggatg cgccggagat cgacggctgc
    11521 gtcttcgtcg acgccgcgac aggtttgaag ccaggcgata aggtgcgcgt cgccatcacg
    11581 gacgccgacg agtacgacct gtggggccgc atcgtcagct aggcggcggg ctcgcctatc
    11641 gcctgggcgg cacgagcttc aagagcgccg cgcggtcgag cgtgaaatcg gaggcgatcc
    11701 acgccgtctc gaggtccttc aggattgcgc cgaagctcgg tcccggactg agacccgcgg
    11761 cgatcagaga ctggccatcg acaggcagct tcgggacggg ccagcgttca ggcagcccca
    11821 agagttcgag ccaggacggt tcatcgatgc ggccatcggc gatccaggcc gcgaacacgt
    11881 gccggcgcca ggcctgggcg cccagccgat agaggctccg ccggcgctcc gcgtcagtga
    11941 gtgtcggcgt cacgtcgatc cctgaccgga tcggcagcag cgcctcgcgc tcgcagccgg
    12001 caagtttgag gtgatcggcg agcctgtcga cgtcctcggc cacggccacg gcgagggccg
    12061 agagccgcag agtggggctc gcggccacgc ccgccgcggc ctcgacggca gcagccttgg
    12121 aaagcaatgc ggggcgtggc gcggcacgca ggatcgacgg caggaggccg caatccatca
    12181 tggccgcgat ggcgctcacc acgttcgggc cgacgagcag cttgcgcaac tcgcctgcaa
    12241 tccgctccat cgacagtcgc tgcaagccgt cgcgctccgc tatggaggcc gcgaggccgt
    12301 ccgcttccat ggcgccgcca ccgagctgcg cgtgaaagcg gaagaagcgc aggatgcgga
    12361 ggtagtcctc gcggattcgg acacgggctt cgccgatgaa gcggatgcgg cgccgggcgc
    12421 agtcggccac gccgccgacc aggtcatgga tcgtgccgtc gccgtcgcaa tagatggcat
    12481 tgatcgtgaa gtctcggcgg gccgcgtcgg tcgcccaatc gcgcgagaag gcgacgcggg
    12541 cannnnnnnn nnnnnnnnnn nnnnnncacc tcgaaggggt caccttcggc gattaccgtg
    12601 atcgtgccgt gatcgatgcc ggtcggcacc gacttgagcc ccgcggctgt tgccgctgcg
    12661 acgatctcga ctggcgtcgc ggtgctggcg atatcgatgt cggtgacagg gaagcccatc
    12721 aaggtgttgc ggaccgcgcc gccgacgata cgcgcctcgt gcccggccct gccgagcgcg
    12781 tcgagcacgg ctcgcgtcga cggcatcgcg agccaggtcg caccggcgag tgttcgaggc
    12841 gttggcatgg tcgcgatcgc tgtctctctt gtcgcgggcg gcggccttac tccgcagcgc
    12901 tctcgcgcag gcgatcatgc agattgcgga tcatgccggc ggtcgcaccc cagatatagc
    12961 gctcgccgta gggcatcgca aagtagtggc gctcgcgtcc gcgccattcg cgcgaatggc
    13021 gctgatggtt gcgcggatcg agcaggaagc cgagcggcac ctcgaagaca tctgcgacct
    13081 cgctcgcatc gggcgtcagc gtgaagccgg gccttataat cgcgacaacg ggcgagatca
    13141 ggaagcccgt gccagtccga tagctgtcga ggaagccgag cggctcgacc tggctggcgg
    13201 caaggccgat ttcctcttcg gcctcgcgta gggcggcagc gacgggcgag gcgtcgcttt
    13261 cttcgacctt gccgcccggg aaggcgatct ggccggcgtg ggtcgaaagc tgttcggtgc
    13321 gctgtgtgag gagcagcgtc aagccgtcgt cgcgggcaac gattggcacg agcactgcag
    13381 cggggcgcgg cggcggcatg acagagaaat cagcaagcat ctcgggattg agatcccagt
    13441 caccgcgacc ccaggagcgc ccggtgcgcg gatcgaagat gtcgtctgaa ggtgcagcgt
    13501 ggaaggcgcg cgccgcaatt gcgcgcagga tcgcctctgt gagggtgatt gcggcggcgc
    13561 tcttatcgtc agtctcctgc atggctccgt cagcgcccct tgaagatcgg cgcgcgcttg
    13621 gccgcgaaag ctgccgccgc ttccttgtgg tcctccgtct cgcgcgcctt ggcgaggttg
    13681 gcactctcgt tgtcgaggca gtcggcgaag gtccccttga gcgcgcggtt caagttgtcc
    13741 ttgataaggc ggtgcgcgac gcgtgggccg tcggcaaagc ggcgggcgag cgccatcgct
    13801 ttcgcctcga gttccgctgc cggcacgacg gaattgacga tgccgaggcg caacgcctcg
    13861 gacatctcga tccggtcggc aaggagataa agctcgcgcg ccttggccgt accgacgagt
    13921 tgcgtcagga gatacgtgcc gccgtagtcg ccggagaggc caactttggc gaaggcggtc
    13981 gtcatcacgg cgctgtcagc cgcgatccgc atgtcacagg cgagtgccag cgacagaccc
    14041 gcaccggccg ctggaccggg aatcgcggcg atggtcggct tgcccatttc gtagaggcgg
    14101 agcgatgtca cgcgctgctg ctggcgctgc agctccatgc gcgtcacggg gtcgggcgac
    14161 ttgcccgagg cgtgcacggc ggccatggcc ttcacgtcgc caccggcgca gaacgcgccg
    14221 cccgcacccg tgagcaagac ggcgccgatg tcgggatcga gctcggcggc cgcgagttcg
    14281 gcgcccaagg ctgcgaccat ggccggcgtc agtgcattgc ggtgctgcgg acggttcatc
    14341 gtcagaacga ggacgccctt gtcacgcttg acgaggagat cgtcggtcgg gctagcggag
    14401 atcaaggtct ggctcatgct cgttccttta ccaggtcggc ggtgcgtgtg gccgtgtcgg
    14461 cgatttcagc cgctgcgggt cgagaaagca gcgaccggcg tcggtcacgg cgacgacgat
    14521 ctcgctgccc gcctcgcggc gctcgagaag gccgcggtcg acggcgtcct cccacaccgt
    14581 gaggcgcggg cacgatgtcc gccaagtcgc catgacctcc tcataggtgc gcggcgcctt
    14641 cgcgcaccac tcgacgagat cgcggatcag gggctcgatg gtcgccgaca tgcctctctc
    14701 caaatcactc agccgatctt atcgcggtcg accggcgctg gctcgaaaat cgtgtcgcga
    14761 tgtgattgca gggatgccca gtggagttcg aggtcctccc ggaagaggcc ctgcgtgatg
    14821 ccccgggcga ggcgcggcgc ggagatcgtc atggcgttga tggagcagcc gaagggaccg
    14881 aagctcatgg tggtagcgat cgtgaagtcg tggatccgcg cgaggaaagg tgcggcacct
    14941 gggcagcgct cgaggaacga gccgtcgggc gcgtgatagg gaaatcgggc gaggcgcggg
    15001 ctctgcgccg tctcgcgcgg gtcgtaacgg tcggcccagg tggcgatctc gccggcgaag
    15061 gaggcgagtt cgggccgggc ggacggatcg atgtcgatgc cggcgccgac gatgaggaag
    15121 tcggcggtga cgtccccggc tgccgttctc agctggacat gatcgccggc ggcctcggcg
    15181 ccctcccaac cggcgcccac atggatcgag aagcccgcgt ggcggcggca gcggtcatag
    15241 gtctcctgcg gcatgctctc gcggagctcg aggatgtggc gcatgaactt ccatcgccaa
    15301 tggtcgtcca tgtcggccat gtgatggagg aagcccgaga aggtcagcca gaggtatggc
    15361 tgcacgcgct ggagcgccgg gcgccggacg aacatcatga ccttggcggc gcctgcttcg
    15421 agcgcagtcg ccgcattgtc ggcggcggag gcgccggcgc cgagcaccgc gattgtcttg
    15481 ccggcgagag ccgcgaagtc gatatcgtcg gaggtggtgg cgcgcaggtg ctgcggcagc
    15541 cgctcgacga aatccggcag cagccagcgg ccgagaccgt cctggcccgt tgccagcaca
    15601 agtttgcgcg tgacgacggt ttcggttccg cagggcgtct cgaggccgag gcgaagacag
    15661 gcattatccg gcgtgatcgc gacgagggtg gtctcgttcc ggaccggcag tgcgaggacg
    15721 gcgcgatacc agagcagata ctcctgccaa tgctctttcg ggatgcggtg gagccgctcc
    15781 cagtgctcac gtccgaagcg cgcctcgtgc caagcctgat aggtgaggct cgcgacgccg
    15841 agatcggggc ccgtgaactc cttcggcgag cgcaacgtcg gcatgcgggc gtatgtcgtc
    15901 cagatgcctt cgcgaccgta ggcggcacgg tcaacgacga ggatgttgtc gatgcgcatg
    15961 cgctggagcg cgaaggcgac ggcgagcccg ccctgaccgg cgccggcgat gacgacgtcg
    16021 tagaggtgtg cgccgcctgg acctttccgc gtcggcatcc aggccttttc gggatggcgc
    16081 gtcaggtgga ggtcgcggct aaccgcagcc tcgagcgagc gaagcctttc accgcccgtg
    16141 gtctcgccgt ccacgatccg tcgtcacttg tgatcggttc tgagccgctt ctcgagccac
    16201 tgcgagtagc gcgagatcgc aaaacagaag aaccagaaga aggccgccgc gaacacgtaa
    16261 cccgtcatcg ccgtctgagg cgcgaaccac ttcgcgtcgg tcaggttgaa ctgcacaata
    16321 ttgagcagat cgaacaggcc gatgatcagg acgaggctcg tatccttgaa aaggccgacg
    16381 aagctgttga cgatgccggg gatgacgagc ttcagcgcct gcggcaggac gatgaggccc
    16441 atcttcttcg ggtaggagag gccgagcgcg tcggccgcct cgaactggcc gcgcgggatc
    16501 gcctggagac caccgcggat ggtctcggcc atgtaggcgc cggcgaacag ggcgacgccg
    16561 acgagcgcgc gcagcagctt gtcgaactcc atccccggcg gcaggaagta gggcaacatc
    16621 accgacgaca tgaagaggat ggtgatgagc ggcacggcgc gaacggtctc gatgaagaac
    16681 accgaggccc agcgggcaat cggcatctgc gagcgccgcc ccaaggccag gatgatgccg
    16741 acagggtacg agcaggtgat gccgacactc gcgacgacaa gcgtgacgag gaggccgccc
    16801 cagcgctcgg ttttcacgac cggcaacgcg ttgtggggac cgggcggcgg gttggtcgcg
    16861 gcgaggccga ggatcttgcc ctggtcgagg gcgacgaaca gcatctcgat cagatagggc
    16921 agcgccatcg tgaacaggat gccgccgagc aggcgcatgg tctgtgggat gaggatctgc
    16981 cgcgcgacga acgagagaag aacggaaaca gcggcggccg aaagcagcga tagcccgatg
    17041 tagggccggg catcggagta gatgcgatac caccacgact gcttcgagcg gatgtcggcg
    17101 agctctgccg ccggaagctc gacggggact gtgacgcccg tgagaagcca ataggcgatg
    17161 atcggaaaga cgatcagcgt gtagtacgct tggaagccct tccacgggat cgccgggatc
    17221 atcaggggga tgatgccagc gagcgccagg atgaaggtca ggctgacccg ccagcgcatt
    17281 tcctcagggt agcggccata gatgaactgc gagaacttgg ctttcacgaa gggccagcac
    17341 gcgcctttcc cttcgccgcg gcaggcggag ccatcctcgc cggtgaagac ggccgagaag
    17401 accgcccagt cgagggcacc ggcaacgagc cggtagagga agaagaggca gacgaccgtg
    17461 aggatcgagt tgccgacgct cgagaacagg ttcttgcgca tccagccgac taaaccggtc
    17521 tcggatgcag gcggtcgcag ggccggggta gcggctgaaa gtgcggtatc aacgggcatg
    17581 ggcgatcacc tctcgaccag cgccatgcgc ttgttgaacc agttcatgaa gagcgacgtg
    17641 agcaggctca gcgtcaggta gacggccatg gtcatcagca cgatctcgat ctcgtggccg
    17701 gacttctggt tgagcacggt gcctgtgaag acgccgacaa gatccgggta gccgatggca
    17761 atgccgagcg aggagttctt ggtgacgttg aggtagttgc tggtcagcgg cgggatgatg
    17821 atgcgcagcg cctgaggaat tacgaccagg cggagcgcct ggccattggt gaggccgatc
    17881 gcgcgggcgg cctccttctg gcccttcgag acgccgagga tgccggcacg cacgatctcg
    17941 gcgatatagc cgcccgtgta gaagaccagg ccgatgagga gtgctgccag ctccggcagc
    18001 agctcgattc cgcccgacat gttgaagcga ccgacggtcg ggtgctcgaa ctgcaccggg
    18061 cggcccatca cgagaaaggt gccgatcggc aggatcagga tcagggcgag cgaggtccag
    18121 agcacaggaa actgctggcc ggtcgccagc tgacggcgcc gcgcccacat cgcgacgagg
    18181 atggaggcga taatgccgaa gacgagggcg aagagggcgg tccggctccc cggcaggaac
    18241 tctggtttcg gcaggaagag gccgcgattg ttcaggagaa tgccgccacc gagttcgacg
    18301 ctgcccggaa cgcgcttgcc gtcgaccatc aggaagtcac cagtcggtga ttgcttgaag
    18361 atgcccggca gattcttgat gacgccgaag taccagatga ggaggtgcag cagcagcggg
    18421 atattgcgcg tgatctcgac atagatcgcc gcgaccttgg cgatgagcca gttggacgag
    18481 aggcgtgcga tgccggcgac gaaaccgagg atcgtcgcaa ggacgatggc aatcgccgag
    18541 acgaagatcg tgttcaggat gccgaccatg aaggcatccc agttcgagga ggcggccgaa
    18601 taggggatca gcgtctggct gatgtcgaag ccggccgagc tgttgaggaa ttcggcattc
    18661 caggcgcggc cctggcggcg ggcggcggca ttggagatga tcatccaaaa gaaggcgacg
    18721 agcgtgacga cgatcgcgac ctgatagatg atctggcgcg tctcgcggcg gaagaagatg
    18781 ctcatgaggc catcatcgga tgcgcctcgt cgcgcggccg gcggtcggcc gtcgtccatc
    18841 actgccatgg tccctccgca acagtcgtgc ccgttgtccg ggtctcggtg actgcgtgag
    18901 ctattgagcg gccaaaaccg tcacctgatg cgtcatcacg cgatgcgacg gcggccttta
    18961 cgagagccgc cggcccattt ctggaccggc ggcacattca ttcaacgtgt cagcgaatcg
    19021 gcggagcgta ttggaggccg cccttggtcc ataggttgtt cacgccacgg gcgatcttca
    19081 gcggcgtctt tggaccgacg ttgcgctcgt agatctcctc gtagttgccg acgcccttga
    19141 taatgcggac gacccagtcc tgcgtcaggc cgagcggctt gccgaagtcc gtctccttgt
    19201 cacggccgag aaggcgcatg atctcgggat ttttcgtgtc ggcgagcatc ttgtcgacgt
    19261 tggcgctggt cacgccgagt tcctcggcat tgaggagggc gaagtagacc cacttgttga
    19321 gcgtgaacca ctgccagtca gcctggcgga cgagcgggcc aagcggctcc ttggaaatga
    19381 tctcgggaag gatcacatga tcttccggct tctgcatcct caggcgctgc gcgtaaaggc
    19441 tcgaggcgtc tgtcgtgtag acatcgcatc gaccggcctg ataggcggca ttgatctcgt
    19501 cggacttctc gaacacgacg ggcttgtatg tcatgttcat cgagcggaaa taatcggaca
    19561 ggttgagctc ggtcgtcgtg ccggtcgcaa cgcagacggc cgcaccattg agttccttcg
    19621 ccgatttcac gccgagcttc ttgttcacca tgaagccctg gccgtcatag tacatgacgc
    19681 cgacgaacgc gaggttcatg gcgctgtcac gggcggccgt ccaggtcgtc gtgcgcgaga
    19741 ggaggtcgac ctcgcccgac tgcagagcgg tgaagcgctc cttggcggtc agcggcttga
    19801 aggccacctt cttggggtca ttgaagatcg cggcagcaac ggcgcggcag aagtcgacgt
    19861 cgaagccggt ccagttgccc ttgtcatccg ggttcgagaa gcccggcgtg cccggaccga
    19921 cgccgcaagc aacggcgccg cgagccttga cgacctcgag ggtcggtttt gtttgtgccg
    19981 aggccaggcc cgtaaaggcg atggcggccg ctgccgccac ggcaccgatc ttgaagctac
    20041 gtttcatcag gtatcctccc ttgacgcaga actggcgcca gcctcgccgc gagtaggcgc
    20101 cccgcggatg taccgcgaac cccggttgac tttgctcaga cggggcatcg accgactatt
    20161 caacggcggc gagccggtgt caaacccact cgaggcacaa tactttcaga tcgcgagccc
    20221 ttcggcgaca tcgaaattca ggcgccacgg cccgaatatc gggcacaatc aacgtcctgt
    20281 gatcgactta aaggcgaaga tgagcagcac caggtcctgc ccgggccggg cgacggccac
    20341 ctcgcggcgc agccacatcg acagatcggg cgcgtagtag tcgtggaaca cgctgcgctc
    20401 gccatcctcg aactcggagt ggcgaatgat cttccacact tgataggtgc aggcgccgat
    20461 cgtgagctcg gtcggctcgt tgtcgacgcg caccttgagc ttggccttgc gcaccttgtc
    20521 gccgaccttg accgtatagc tggtcgccaa ggtcgcgccg cccttgaccg gatgcagaga
    20581 ccggaaatcg ccgtcgagct cgagctcgaa agcgacccgg cccgacagct tctgctgcag
    20641 gatgccgcgc caggtcgtca gctcctccga cacgaagctc gggttgcgcg ggttcgacgt
    20701 gaagatgcgc acagtctcgc tgtcgaacgg ctgtatgtcg tgatcggcca tgcgcggatc
    20761 gtgcgcttca agcttgaagc cgcgcgacgc ggtaacggct gtcgggcaat ctgcgttcgc
    20821 agcagggctc gaggtgatga ccgcgccgaa tagcattgcg gtcacgagag caagtcgcat
    20881 gaaactctcc gtattcgaat aggcgcacgg agtcttgccg aaatgtcggg gcccgtcacc
    20941 tgtcggtgcg ctcgaacaac agcttgccgc cgctctcgtt ccgccgcgcg cgaccggtcg
    21001 tcacgagccg atggatatgg gcaagcgtct cggcgagcgc gtgccggcct tgcccttcgt
    21061 cgacggcctt cggaaagaga cctcgcgcga gcgtcatggc gacctcgggg cggcccatca
    21121 gcccgacgag cgtgtcgagt cgagcctcgt gatgatctgc gagctggtcg acgcgcacgt
    21181 ggagcccgcg gaacggcatg ccgtgcgatg gcaggacgag cgtgtcctcg ggcaacacac
    21241 gaaaacggtc gagcgaggcg aggtattcgg tcagcgggtc cgaccacggc tcctgcggga
    21301 agacgccgat catgggcgag atcttcgaga gcacctggtc gccggcgatc aggatgcgct
    21361 cttcctcgca atagaaggag gcgtgctcgc tcgcgtggcc gccgcacacc atgacgcgcc
    21421 aggcacgtcc gccgaagcgg atcgtctcgc cgtcggtgat gcgcaggaac gacgccggca
    21481 ggggcccgag cagcgcatgc gtcttggcgc ggtcctgcgc gtaggcggca acggtcggct
    21541 gatcgcagcc gtggctctcg aggaagcgca tcgtatcggg ccgcattggc tgcttggcgt
    21601 cgatgatgcg gacctgcggc gaatgccact cggcgagcgt cgcgacgaac ggcacgccgc
    21661 cgacgcgatc gacgagccaa ccggcgaggc cgacgtggtc cgtgtgaccg tgcgtggcaa
    21721 tcaggcgacg gacgggtgca ccggaaagcg ggcccgacag cagcgcctcc cacaggtcga
    21781 gcgcctcgcg cgtcgtcgtc gccgtatcga tcaacgtcca accatcgtcc tctcggagca
    21841 gccacacatt gatgtgattg agccggaacg gcagcgggaa gcgcgcccag agaaggcccg
    21901 gcgccagctc gatcgtctcg gcgcccatcg gatggcgcgt gtgcgtgtag tgaagctgcc
    21961 gatccgctcc cgacatcacg tggtctcccg aggttctctc gcctcttacc gcagcgcaac
    22021 gagtgtggct aggcgacggc ggcgcggggc agcctgcatg aacggcatga cgcaagcgaa
    22081 ggcgcaacag cccagggaca acagcgcact gtcaagcgca tcaaaatcaa ctttggaatt
    22141 ctgaattcag tatgcaataa tccgctcgac aagggaggcg tcaatgcagc tcaccagcgt
    22201 ccaggccgcg ccacagctca tcgaccaggt ctatgaccgg ctgctctccg caatcgtcga
    22261 cgggacgctg gcgcctggcg agcggctgac gcaggaaagc gttgcggcgc tgctcggcgt
    22321 ctcgcgtcag ccggtgagcc acgcacttca ggtgctgcgc cggcgcgggc tcgttgtcga
    22381 gaccggcaag cgcggcctct ctgtcgcgcc gatcgacggg gcgcgcatcc gcgatctcta
    22441 tcaggtgcgc gaggcgctcg atgccatcgc cgcgcgcctt gccgccgaac gggtcgttac
    22501 cggggcgctt tcgaccgagg agcggcgcga ggccgaggac tgcctcgcct gcggcgcgcg
    22561 gctcgccgac ggctgcccga cggccgagct gatcgcggcc gacgtcgcat tccattcgac
    22621 gctctacagg ctcagcggca atggcgcgat cgcagagacg gtcgccgagc agtggccgca
    22681 tttcatgcgt tccatggggg cggtgctcgg cgaaggcggc gccgttgtcc gcgtctgggc
    22741 cgagcacgcc ggaattctcg aggccgtact cgccggcatt ccgcaccttg ctgcggagcg
    22801 cgcccgggca cattccgccc gcgctggcga ggcaaccgga aggcgcctcg aggccctgtc
    22861 gcaatccgcc gacgccgccg tcgtgcggcg atccattcct gcctgagact gcaaccacaa
    22921 cgaggagacg acccatgaag ctcacgacca agcagctcgc cgacttcgag cgcgacggct
    22981 acatcatcat cgagaattgc ttctcggatg ccgagatggc ggtgctgcgc gcagaggccg
    23041 aaggcatcta catgaccaac cgcgaggagg tctggcgcga gaagtcgggc gcgccgcgca
    23101 cggccttcgc cgcccacaag tacaacgagg cgttcggcat ccttggccgt catccgcgcc
    23161 tgatcgagcc gctcgagcag ttcttcggcg agagcgtcta catgcatcag ttcaagatca
    23221 atgcgaaggc gccgttcgac ggcgaagtgt ggcagtggca ccaggactac ggcacctgga
    23281 agcgcgacga cggcatgcct gaagcccgcg ccatgaatat ctcggtgttc ctcgacgagg
    23341 tctatcccta caacggcccg ctgatgttca tcccgaagag ccacaagggc ggcacgctgg
    23401 cggccggcca cgacctgtcg acgacgagct atccgctctg gacgctcgac aacgagcagg
    23461 tgacgaagct cgtcgcggag ggcggtatcg tcgcgccgac ggggaagccc ggcacggtgc
    23521 tcatgttcca cggcaacctt gtgcacgcct cgccgccgaa catcacgccg tacccgcgta
    23581 agatcgtgta cctcacgctg tgcgccgtct cgaaccacat cactcagttc aagcggccgg
    23641 agttcatcgc gcatcgcgac ttcacgccga tcaaagcctg cgccgacgac gcgctcagcg
    23701 tctatgtgcg gaagtacagg caggccgcag aatagtcacc gagggctcgg ccccttgtcc
    23761 ttcgaaaggg ggttccaagg gccagccctt ggcggagtgc tcgaggggct ggcccctcga
    23821 gagccatgcg caccgaattc tcgtcacgat ccgagtagga aaaccttcga ctggccgcta
    23881 aactgatcgg gtacgtcagc tcccattcat tccaggggat gccatgtccc gcctcatatt
    23941 ccgtctcgca gtctgcattg ctctactcat cgccgtcgcc gctcaggctt cggccgagaa
    24001 acgtgtggcc ctcgtgatcg gcaatggcgc ctatgttcat gccggccggc tgccgaaccc
    24061 gcccaacgat gccgccgaca ttgcggaaaa gctgaagcag ctgaaattcg aagtcgttgt
    24121 cgggcgcgat ctcgacaatg cggggatgcg ccggatcatc cgcgacttcc gcgtgcgggc
    24181 agagggcgtc gatgtcgcgc tactcttcta tgccgggcac ggcatgcaga tcggcggcgg
    24241 caacgtgctg ctgcctattg atgccaaggt ggacgatgag ctcgatgtcg agccgcagac
    24301 catccggctc gatctcgtcc tcgccgaaat ggagcgcgcc gcgcgcgtca acgtagtgct
    24361 gctcgatgcc tgccgtgaca acccgctcgc cgagcggctc atgagaaaca tcggcgcacg
    24421 ctcgcgcagt ctcggtctct cgcgcggcct ggccaaggtc gatgcccgcg gctccgacac
    24481 gctcatcatg ttcgcgacgg caccgggtga cgtggccgcc gacggtcgcg gccgcaacag
    24541 cccgttcacc gtggcgctgc tgaagcacct ggccaagccc gaggagctga gcgtcgtcct
    24601 caaggatgtc gtcgccgacg tgcgcgcggc aaccggtggc cagcagcgcc cgcagcaact
    24661 cgcggcgatg gagcgcaagc tctacctggc gggcctgccg gcgtcgtcat cgggcgatag
    24721 cggtgagacg gccaagctca aggcggagat cgaccggctg cggctcgagc gcgaacgcat
    24781 ggagcgcgag cggacggagg ccgagcggaa atccaagctt gctgctgtcg cgccgtcggg
    24841 tccagcggtg ggcgcggcgc ggctcgaccg ggatatcgcg ctcaagatcc agtcgattct
    24901 acctctcttc ctgccggtgc tcggcacgca gcccgagcgc tttgccgccg cgctttcgcg
    24961 ctcggcaagt gcggccggcg cgagcgcgaa agtcgaggtc gcgcctccga actccgtcgt
    25021 accgttctcc gagatactga gcgcgctcaa tagtggccgc ctcgacgcgg catggggcgc
    25081 aatgccgatc ttgatcggca gggacaagtc tttcgagatc ttttcgggcc aggtgccgtt
    25141 cgggcacgac ccggcgcgtt tcgtccgctg gatagagagt ggcgggcggc cactcttgaa
    25201 tgggcatgcc cgcgctctcc gcctccacgt cgtgccctgc gcgatcgtcg gtcaggaggg
    25261 cggggggtgg ttcaagaagg cgatccagcg gcctgaggac ctgcgcggtc tcagggtccg
    25321 ctggtacggc agcgggaact cggtcatgca gcgggtcggg gccctcgcgc agtcgatcgg
    25381 cgctggcaac gtccactcga tgctcaccaa gggggaactc gacggtactg agttctcgct
    25441 gccgagcgtc gatgccacca ttggcttcca cacgatcgcg cggcactact atttcccggg
    25501 ctggcatgcc ccggcattgc tctcggacct gacgttctcg cgcaccgcct gggacaagct
    25561 cgggcctgcg acccaacgtg tgatcgagga gacgtgcagg cacttcctcg cgctgacgct
    25621 ctccgagatc gccaaggacg aagccgaggg cctcgccgag atccgcgcga agccaggcgt
    25681 cgagatcagg ccgtatccgc ccgccgtgct tgcggtcctg cgcaaggccg caaacgagat
    25741 ccaggccgag acgagtgcaa acgatgccgg ctacaaggcc atcctcgaca gctatcgggc
    25801 agcgcgctga cactgccgtt gaccaagtgg cgagcatccg agccgtgccc gtgttgacag
    25861 gcgaccggcg gcgccatacc tctcggttat ggccagggca cgacgagtta tgaagaagcc
    25921 gcgcaacgca aagttcgcga tcggatcggt ggtgcgccac cnnnnnnnnn ngcatctatc
    25981 cgttccgcgg catcattttc gatgtcgatc ccgtgttcgc ctcgacggag gagtggtggc
    26041 aatcgatccc gcccgacatc cggccgaaga aggaccagcc attctatcac ctgctcgccg
    26101 agaacgccga gaccgagtac gtcgcctacg tgtcggagca gaacctgctg ccggacacct
    26161 cgggtaagcc gttgcgacac ccgcgcgtga aggagctctt cgcgcgcgag ggcggccgct
    26221 acaaagagcg cttccttcag cggcactgag cggcgaacgg aatgccagat ggcgcatttg
    26281 cccgtcaggc ctggcgatca taccaacggc tcatcagctc catcaggatg acggccatcg
    26341 cgctcgaggc gagcagcagg agcccggccc cggtcgggcc gatgcgagcc acgatccacg
    26401 gatagatgat cggcgaggcc gcgctgacga ccaggatcgg cgtcgcgatg agcccgagca
    26461 cggacgcgta gccgcttgcc ccgaagagcg cgagcggtac cgcgccacgg acgatcgtca
    26521 gcacgccttg ggcggcgccg gtgagcaccg tgaaggcaac ggcgagccag aggcttccgc
    26581 cgctcatgat cagcagcaca accgaggcgg cgagcgaggc gatggcgatg cgcgcgatcg
    26641 tcatcggatg gtagcggctg ccgtaggtaa gttcgaggat gcggccgccg acctgggcga
    26701 cgcccttcaa tgagccgagc cataccgcgg cggcggtcgc gaggcccgcc tgctcgagga
    26761 ggtgtacgag ttgcaccgac gtcaccgaga atacgaaggc aatgaacgac atgacgagcg
    26821 cgaagagtgc gatgccgacc gaccgcgcgc gaccttcgag cggcctcccg tcgaccgcgc
    26881 tcgccgctcc ggccgccgca ggcttcgcct ggccggtgtc gctgcccttg tcgtgccgcg
    26941 tgatcccgaa ccagtggagc ggcacgcagg cgacgagatt gaacaccgcg aaaatggcga
    27001 gcgtctgccg ccagccgtag aggccgttca ggtaatggcc aatcggccag aacacgctcg
    27061 aggcgaagcc gccccagagt gtgagatagg agatgccacg ccggccacga tccggtgcca
    27121 cctgcacgat cgcggcgaag gcggcatcgt agagggtgag ccgcatggcg atgccgaggt
    27181 aggcccatgt gatgaggtag gtcatcagat cgacgacgcc cgaaagcgca tagaggccga
    27241 tggagacggc gactgagccg gcggtcatca ccgcgcgggc gccgtaggtg tcgatgagaa
    27301 agccggtggt cgtcgaggca gcgctgcctg aaaggagtgc gacggcaaag cccgtgaaga
    27361 cggtactcgc cgaccagccc atttcacggc cgatcggcgt cgagaggacg cccaggcagt
    27421 agtaagtcgt accccaggcg atgatctgcg tgatgccgag gcacgttatc gcccgggcaa
    27481 gcgcatatcg gtccggcgct gcgtcagtcg ctatcacgcg tgcccccgtg ttcggctgca
    27541 gatcctctgc cgccgagagt agcgtcattg tcgcgggcga tccacgtgca tgcgccagaa
    27601 tgatatatgg tcgcggcggc gggcatgcgt gcatgcccta cctgccggaa ggagccgtgc
    27661 attggcagac gacgaggcaa atcggttttc ggcacgcgcc gcgcgctatg tgcgcgtcgg
    27721 caccaacgtt ggtgcggtcg ctgcccgcgt cgctggcgcg cgcctcttcg gcatggatct
    27781 cgaccgccag aagaacgcgg cagaactcgc caaggctctc ggtgggctca agggtccgat
    27841 catgaaggcc gcgcagctgc tctcgaccat tcccgaggcg ctgccgcccg agtatgcgcg
    27901 ggagcttgcc cagctgcaat cacaggcccc gcccatgggc cccgccttcg tgcgccggcg
    27961 catgcaggcc gagctcggac cggactggca ggcgaaagtc gcccgcttcg aggcggagcc
    28021 ggcggcgtcg gcctcgctcg ggcaggtgca tcgcgcgacg agccatgacg gccgctcgct
    28081 cgcgatcaag ctccagtatc cggacatgca gtcggccgtc gaggcggatg tcacgcaact
    28141 gaaggtcgtg ttcgcgatcc acgcgcgcat ggaccccgcg gtcgacactg ccgatatcct
    28201 gcaggagatc gccgcgcggc tgcgcgagga gctcgattac accctcgagg cgcggcacat
    28261 cgcgctctat gccgcgattt tcaagaatga cccgcgcgtg cgcgtgcccg aggtcgtccc
    28321 cgagctgtcg acaaagcgcc tcctcgccat gacctggctc gaggggcgac gcctcctcga
    28381 cttcaaggcg tcgcccgtcg aggaccggaa cgagatcgcc cgcgccatgt tccgcgcctg
    28441 gtggttcccg ttctcgcact acggcgtcat ccacggcgac ccgcatctcg gcaactacac
    28501 gatcttttcg ggtgacgatg gccggccgcg tggcgtgaac ctcctcgact acggctgcat
    28561 gcgcaccttc ccgccgcgtt tcgtgcaagg cgtgatcgac ctctacaacg gcatcctgcg
    28621 caatgatcgc gctctcatcg tgcatgctta cgagacgtgg ggctttcgtg gcctctcgaa
    28681 cgagctcatt gacgtgctca acatctgggc gcgattcatc tacgggcccc ttctcgacga
    28741 ccgcgtcagg gcgattgcgg aaggcacgac ggcggcggag tacggccgca aggaggcctt
    28801 cacggtgcat caggcgctga aggaaaaggg gccggtgaag gtgcctcgcg agttcgtctt
    28861 catggaccgc gccgcgatcg gcctcggcgg cgtgttcctc cacctcgacg cgcgcctcaa
    28921 ctactaccag atcttcaatg agaccatcga cggcttcacc ctcgacgccg tcaccgcccg
    28981 tcaggccgcc gcgttcgcgg ctgcgggcgt gccgctgccg gtggcgtcgt tttgaagggg
    29041 atcagaaggg cttgtagccg agcgcgaaga ccgcgagtcc gatcaaggcc agcaacaggc
    29101 cagcaacagg ccagcaacca tgcgaacggc cacgtagttc gtggcctgca tgacgatgtt
    29161 cccgaaccag ttgccgagtg ctgacgcagc gcgacgaacc accagccaga tgagctcgcg
    29221 attgcaatag agcaagtcgt ccacggcagc cgagaaccta gccgcaccag cgagcgtgta
    29281 caaagcaatg atagtcagga aacccacttc gatgacctgc tccgccatgt cccgtgcctc
    29341 caagtacata catatgatta tcagatttct atagtgaatt tcaatttcac gtccagtgaa
    29401 aaatctgacc tcacgatcat gtgaccgtga ggccgtgcgt gtgccgactt atggaagcag
    29461 cttcgtgatg gccttgatcg cgaggcgcag gtacgcctcg cggatctccg ttgtcggcgg
    29521 cagatgcgcg ccacgcaccc atcggccgaa cgtcgccgga ctgatgccca tctcgaggct
    29581 caggtccttg tgtggaattc cggttccgtt gagaatgacg aaggccagct ggaaatcgcc
    29641 gtccttcttc ggatcaagtt tgtgaagcag tgcgaccttt gccttattca gtttctcgag
    29701 gtcactccgc gcaatacgcc cactcgaaga caaatcggcc gccgcaggga gtgcgacggc
    29761 gcttcgtgct gacataaaag actccttcgc cagaggtgac gccggaatgt ggggtggcgt
    29821 cacgttcgcg gttactgttg acagtgttgt cgcgtcggcg gtccatgcca ttcccccagt
    29881 tgcgatcggc acggatgtac gaggcgcgat gcacggcaat atacttcaat gaaattgatt
    29941 tgcaagcaac atcttgtcat ggtatatgag gtgtcactga atcgatgcat tcagtccgtt
    30001 cgagcttggt aggatcgctc aggcaggttt ctaagtcgcc tttgtccgtg aggtgtgaca
    30061 tggcgtatcg gtctacgatt gttccgcgat ggattgacga cgaagagcgc gcgcgcgatg
    30121 cgcactggta tccggctgga tgcgcaatcc gcaagttgac agggcgaaag attgcccacc
    30181 ttgccatgga aatctggacc gtcgactcga gcttgccggg agcagcaccg tttcgcgact
    30241 actcgagttc ggcctacagc tggcgatgtg attccggacg tcaagccgat tgctttgccg
    30301 acagccggca tcacctctat cgaggcaagt gggcgcgact tgccggacaa tgcatccgcg
    30361 agctgatgct ggccctgcga gcgctctacg cggcggggca gccatcgcga ccggtcctcg
    30421 cgccaggttt cgtgggtatg cctcgcgtat tttccgagaa cttggcgttg tcgttccaaa
    30481 ccccgatgaa ccaatcggcg ctcgatgccg atgctgagct ggcagcactg tccatgctgt
    30541 cctcgacttc tgccggctat ctgggaacgg gcttgcacga gctagtcccg accaagatga
    30601 acccctgggc gctcgatgtt ctcgagcgct tcttcggacc aagcctcacc ggtggcacca
    30661 ttggtctgca gtacggcgat catatgcaga agaccagcaa gctcgtctgc aattggccga
    30721 agctcggtga agctcgggcc ttgctcaagt aggtctcggc gtgacggatg aacgccccag
    30781 ccacgccctc acggccgccg cagccacggc aagcgcgtct actgagccgt cggcttcacc
    30841 ttaccatggc tccccggccg agccgagcga gagccgggac ttgacgcgtt cgatgggcaa
    30901 ggagcgcgac tcctcgcgcc ttcgcactcc ggccggggaa ccagaacgga gaatggactg
    30961 tcaccgggca gcggcatcgc tgatggtctt ggtgctggtc cgccgaccct tcaccggcta
    31021 acgtgtgcgc tcggccgcgg tggagatcgg cacgggggta cgcttgctcg cactgcagag
    31081 cctcatcggc atcgtgggca ttccgctgct cgcctggctc gcgagcgcca atcgcgcggc
    31141 atggccgccg cggcggctgg ccatgctcgt ggccgcgggg ctcggcgcgc agctcctcat
    31201 cgcgctcgtg ctgctgaagg tgcctcaggc tgccggaatc ttcgatgtgg tgacgcggct
    31261 cgtccgcgcg ctgcagcggg cgactgaggc gggcgtgcag atggtgttcg gccatcttgc
    31321 gggcgggccg ccgccctaca gcgtcgccgc gccgcagaac acgttcgttc tggcgctcaa
    31381 cgcgctcccc ttgatcctgg tgctgagcgc gctgacgcgc gtcctctatc attggggcgt
    31441 gctgcagcgc gtggtgggcg gctttgcggc cgtattccag cggaccttcg gcatcggcgg
    31501 gccgctcgcg acagttgccg cggcgaagat cttcctgggc atggtcgagg cgccgctgct
    31561 ggtccgcccg tatctcgcga gcatggggcg aggcgcgctg ttcgcggcca tgacgctcgg
    31621 catggcgacg attgccggca cggtgatggc gctctacgcg ctgatcctcg atccggtgct
    31681 gccgcgggcc gccggccacg tgctcgccgc ctccttgatg aacgcgccgg ccgcactgat
    31741 gctcgcggcg atcgtcgtcc cggatggctt ctcggacggc ccgaagtccg ccgagatccg
    31801 cctcgaaaat gcgccgcgct cgaccatgga tgcggtcgca cagggcacgg tcgatggcct
    31861 caagctgctc gcggccgtgg tggcgatgct ggtcgtcatg gtggcactgg tatcgctcgt
    31921 gaacgaactc ctgagcctct tcggcacgcc cggaggacga ccactcacgc tgcagcgcct
    31981 cctcggctac ctctgcgcgc cgattgcctt cgcaatcggc atcccgtggg gcgaggcagc
    32041 ggcggccggc agcctcatcg ggcagaagat cgtgctcaac gagttcctgg cctttctcga
    32101 tctcgcccgg acgccgcagg gcgtactctc ggagcgttcg cgcctcatcc tgacctacgg
    32161 cctctgcggc tttgccaacc tgggctcgct cggcatcatg atcggcggac tgacggcgat
    32221 ggtgcccgag cggcgcgacg acatcatcgc gctcgccccg cgcgcaatga tactcggcgc
    32281 gctcgcgacg ctgctctcgg gcgccatcgt cgccctcgtc ggggcggggt gaggcgcgtg
    32341 tcgcaggcat ttgcagagat cggcggctac gcactggcgc tcgcgatcgg ggcggcgggc
    32401 gcgctcatct tcgttgcctt gggctttccg ctcccctggt ttctcggtgc actgaccgcc
    32461 tgcctcgtcg cctcggtgct gggcgcgccg atcaagcggc cgaagccgct ctccgatccg
    32521 atgcgcgccg tgctcggtgt cgccgtcggc tcggccttta ctcccgcgct actcgggcga
    32581 gcggggggca tggtcggcag cttgctcatg ctcgtgccgt tcatgctggt gatcatcctg
    32641 ttcagccgct ggttttacga gcgctttgcg ggcttcgatc ggccgacggc gttcttctcc
    32701 gccattcccg gcggcctcac cgacatgacc acgatggcgg aggaagccgg cgccaaccag
    32761 cgcgccgtca tcctcgtgca ggcaagccgc atcctgctga tcgtgttcac gctgcccttg
    32821 tggctcaagt ttcacgatgg cctcgccgcg agttcggcct ttgcggggcg cgcgcggctc
    32881 cacgagatcg ggttcgtgga tgcgggcgtg ctgctggcgc tcggcttcgc aggcgtcgtc
    32941 gtcgcgcgca ggatcgggct tgccggcgcg ccgatcgtcg ggccgatgat cctgagtggc
    33001 ctcgcgcatg cgctcgggct cacgtcggcc aggatgccgg tcgaggtgct gaccatcgcg
    33061 caggtaagcg tcggcgtgct gctcggctgc caattccgtg ggctcacctg ggccgagttc
    33121 cgcagcacga tggtgtgggg tctcgcgaac acggcgcact cctcgtcctg acggcgggcg
    33181 tggtgatcct cgtcgcccgc ctcaccggct tcgaccaggt ctcggtgctc ctcgccttcg
    33241 cgcccggcgg acagacggag ctcaatctcc tcacttacgt gctgggcctg gacgtcgcct
    33301 atgtggcgct ccatcatctg gcccggctcg ccattgtgat cctcggggca cagatggtgt
    33361 ttcgcgcgca gagtgactgg cggaagaacg gctgaacggg aggggtacgg atgaaagcgc
    33421 tggtctacac aggccccgag cagatggtct atcgcgatga gcccgatccg caggcgaacg
    33481 acgatgtcat cgtgcgagtg ggcgcctgcg gcatctgcgg ctccgacatg cacgcttatc
    33541 acggccacga tgcacgacgc ccgccgcagc tgatcctcgg gcacgaggct gccggcatcg
    33601 cgacgaacgg cgcgttcaag ggccggcgcg tcgccatcaa tccgctggtg ccgtgcggct
    33661 cgtgcgacga atgtctcgat ggccgcccgc acgtctgcgg cacgcgccag atcctctcga
    33721 tgccgccgcg ccccggcgcg ctcgccgagc ttgtccgcgt gccggagcgc aatctcgtgc
    33781 tcgtgccgga tgggcttgca accgaagttg cagccctcgc cgagccgatg gccgtcggct
    33841 atcacgccgt aggcctcgcc gaacgcgcct tgccgcgacc gctcgccgcc gcgcgcatcg
    33901 cggtgatcgg cggcggcgcc atcgggcttg gaacggctca gatcctcgaa tgccggggcg
    33961 ggcggggcat caccatcgcc gagacgagcg agattcgccg tatcgcgatg aagaaggcgg
    34021 ggcgcttcca gccctacgac ccgcgcggca gtggcgcgcc cggtgccaac agcatgcatc
    34081 tcgtgatcga tgccttcggc ggcaagtcga gccgcgagga ggcctgccgt atcgtgaagc
    34141 ccggcggcgt gatcatccac atcgggctcg cctcgggcga gggcggcgtc gatgtgcgga
    34201 agctcacgct gcaggaggtg agcttcatcg gcagctattg ctataccatg gtggacttcc
    34261 gcgagacgct cgccggcctc tccagcggtc agttcgggcc gatcgactgg actgaggagc
    34321 ggccgctcgc tgagggcgtc aaagccttcg ctgaactcaa cgatggcaag gtcgcggccg
    34381 gcaagatcgt gctgaggatg tgagccgtgg gtagagcgtt atgatcgtcg tcgaaaaatc
    34441 ggctgctgcg accgccctgg tgatcacccg gccgacccgc cgcaacgcgc tcgatcagac
    34501 gacactcctg gcgctgcgcg agggcatccg cgaggccgag gcggatactg catgccactg
    34561 cctcgttctc aggggcgcag gcggcacatt ctgctcgggc cgcgacctcg gcgatgcccg
    34621 ccgcgacgcc ccgctcgagg agctgctcgc ctacgacgag acatggggcg acatcatcgg
    34681 cgggctccgc gccatgacca agccgtcgat cgccgtcgtc agcggtcatg ccgtagcggg
    34741 gggcttcacg ctcgccatgg cctgcgattt cgtgctcgcc gagcgcgggg cgcggttcgg
    34801 cgccctcgag atgcggggcg gttttcccgc agccgtgaac accgccgtgc tgacgcatct
    34861 cgtctcgcgc cggatcgcgc tccgctatct gctctcggac gagactttcg cggccgagga
    34921 attgctcgcg cacggtctcg tctccgagat cgccgatggc ggcagcgcgc tcgagcgtct
    34981 cgaggcggcc tctgtcgagc ggctcacgcg cctcgatcct acggccgtca agctcaccaa
    35041 ggaggcaaac cgctcgatgg cggcgagccc cgaggacgtg gcgatccgct ccggcagccg
    35101 nnnnnnnnnn nnnnnnnnnn nnnnggccgc atcttggcgg cgcgcgacag gcacagagag
    35161 cgcaaggccg gcaactgagc cggcgctcca cgtgcgacag actggcctct cgatcaatca
    35221 gacgaacaac ccagtagcgc gggccgcgct tttggttata ttcgcattca tgcatataag
    35281 gggatggaaa ccatggtgaa gccgatcctc atcgtcggcg gcggtccggc cgggctcgcg
    35341 gcggcccatg cactgtcgtc agtcgggcag atgtgcgcgc tggtcgagaa gtctgcagca
    35401 cttggtggcg ctccgatcca atcgggctac gcgaagctcg tgccgtcggg gctctgggcc
    35461 aaggatgcga tcggcggcat ggtctcgcgt gtcgaggcgg cgagccagta cgtgcaggtg
    35521 ctgaagggca cgacggtgaa gtcgttcaca ggcacggctg gaaacttcgt cgccaccatg
    35581 tcggatggcg cgaccggcga gttcggtgcc gctatcctga ccacgggctt cacgcacttc
    35641 gacagtgtga acaagcccga gtggggcttc ggcacgttcc ccgacgtggt caccacgacc
    35701 caggtcgagc agatgatcag ttcggggaag ggcgtgcgct gcccctccga cggccgcaag
    35761 ccgaagcgcg tcgcaattct cctctgcgtc ggcagccgcg atcgccagat cggccgcgac
    35821 tggtgctcca agatctgctg caccgtgtcg gccaacatgg cgatggagat ccgcgaggag
    35881 ctgcccgatt gccatgtcta cgtctactat atggatatcc gcaccttcgg acactacgag
    35941 accgactact attggcggag ccaggaagag ttcaaggtca agtacatcaa ggcccgcatc
    36001 gccgaggtga cgagcgacgg gaagcagctg atcgtcaagg gcgaggacac gctggtgaag
    36061 cgtccgatca ccatcccttt cgacatggtc gtgcatgcga tcggcatgga cccgaacgtc
    36121 gacaacatga cgctgtcggc cgtgttcggc gtcgacctgc acaagcacgg ctacatcgcg
    36181 cgcaaggaca cctatggcgt gatgggtgcg acgtcccggc caggcgtatt cgttgccggt
    36241 gccgccatcg gcccggagac gatcgatgac agtatcgcgc aagcaaatgc tgcggcattg
    36301 tcagctcttg cggccgcacg gggcggcgca cgagccatgg agactgttgc gtgaacagat
    36361 tatttcggaa ggctgtggtc gttctgcttg cggcgacggc gatgaccgtc cttccggcaa
    36421 cggcgcagcg cgcaaaaagc tatgatgtgt gtttgagccc ggcagaatat ctgcgcctct
    36481 acggcgccga ggccgcgccg ggagcctgcg cggcatggcc agagccgtct cctgccgaac
    36541 tcgagatggc catttgcagg gcagctgggc cggacacctg gaagctcgag ctcaagggct
    36601 ccgctgccgc aggtggcccg acgtcattcg aactcgcggc cgttcgcctc agagcgtcac
    36661 tgcccattac cttcagcacc gagctgcagg gcgccggcga ggtcggattg cacatctaca
    36721 cactcatcga cctcgagctc ggcgccgagg agatcgaccc gacgcggcgc tccgaggtcc
    36781 tcgccggcga gccgcgcatc gagcggctca ccaaaatcga tggccgctgc gaggcggcgc
    36841 agctgactcc cgcggccacc ctgctggcac tgcccgcctg ggtggaaggc cctcagtcgc
    36901 ggagggacaa atgatctctg ccgccttgac gtcgcccgat acgggcctgc ttcgaccggt
    36961 gcttcacctc tccggtccga agctgcgcgg ctcgcttgag gggctcatca aggcggcgga
    37021 ggagctcggc ggcatcgaga gtttcgttgc cgccgtgaag ctcaagtcag agctcttcca
    37081 ggagcggctc ggggaggggc gcgcggcggc gatcgagcgc ggagccttcg acgatctcat
    37141 tcgcttcatg ccgacggtgc ggcgacgcat ttcggcgctc gtcgcgaccg acgcctggtc
    37201 cgggacgcgc gcggccatgg cggagctgct gcaggacgcg catcagccag gcaccggaga
    37261 cgcacgcatt gcggccttcg aaggcgcgct gcgcgaaagg gcgacagcca ctgatggcgc
    37321 gcacgcgcgc ttccatcgcg acttcgcggc cgagctcctg cacaacgtct ggcccgagca
    37381 ctatcccttg atgacgcgct gggtgtggga cgccaaggcg aatacgggcg tactgcgcga
    37441 gatctggcat gatcccatcg ccggcgatgc gaccgaccag atcgtcattg acgcgcccga
    37501 cacgcacgac gcgttcctcg tgctgcgcga ggagctgtcg catttcctct ccgagcaggg
    37561 catcttccgc gacatgctct ggttcgtcga cctcgtcgag gcgcacatct acggcggtta
    37621 catcaattcg cagggcggcg cctggctcaa ggccgacttc tcggccgaag gcgatccgct
    37681 cgagcatacg cgccgcatcc tgggcctcga tgcccgcagg cggccctcgg ccggcggata
    37741 gaaggatcca cgcgatgacg atgcgccttg ccccgccctg ccacacgctc ctcacgcgcc
    37801 gcgctccagg aggctgctga tgcccattca cgagaagagc ctgatccggc cggagaacct
    37861 caagactcac gacaagctcg tgatcgatgg tgtcgacgtc tccggtcact ggtcgacgtt
    37921 catcacatcg cgcgtcgtca ccgactacaa cgaaaacctc gaggacgaga tcgcggccat
    37981 gcccggcggc gacaagatcc atcgttgctg gcagtgcggc tcatgcacga atgcctgcac
    38041 gatcaatgcg atcgaaccgc gctttaaccc gcgctactgg atctatctaa tccgcatggg
    38101 ctacgagagc gagcttctgc gcgacaagga tatcatctgg cagtgtgtct cctgccacaa
    38161 gtgcacgaac atctgcccac gcgacgtcga gcccgaaggc gtcatgaagg cgaccgcgca
    38221 ctggctgcac atgaagggct acacgccacc ctcaccgtcg cacatcttcg acgaggagtt
    38281 ctcgcgtcag gtcgtcgagc gcggcaagat cgaggacacc gaggtcctcc acaaattcct
    38341 tcataagacg aagcagccct tgatgcagga ctggctcgtc gcgatcggca ccgggatcgc
    38401 caagcggctg ccgttcgtgt ggggcatcaa gatgatgctc gcgcagctct tcaagccgcg
    38461 gacgcgcaac tggtcgaagg cgcgtgcggc gatcgaggac tacatccacg agaaggaaga
    38521 cgagcgcaga gcggtgatcg cgggatcgcg caaggcggcg ccaccggcgc atgcaggaaa
    38581 ggccggtcac atgatggcgg cggaatagct ccatgctgtt catggtcatc gagcgcttca
    38641 aggatgcaga tccgaaaccg gtctaccggc atctgcgcga tcagggccgc ggcctccccg
    38701 acggcctcaa gtatgtcgcc agctggatcg agccgaattt cgatcgctgc ttccaggtga
    38761 tggaatgcga cgacgcacgc ctgctcatgg agtgggcgat gcattggcgc gagcatgcca
    38821 ccttcgagat cgtacccgtc gtgccgtccg ccgagacgcg cgcgctggtc gagaaaagcc
    38881 tggatcagga gaagcggtga catggtcgac gtaaccgaaa aagctaagaa ataccgctcg
    38941 gtcgcctact atccgggctg cgcgctcgag ggcacgggca acggctacga tgtctccacg
    39001 cgcgcgatcg ccaaggagct cgggctcggc ttgaaggatg tgaaggactg gaactgctgc
    39061 ggggcgatgg aggtgaagaa cgtcgacccg aagatccaga cctatctctc cgcgcgcgtc
    39121 ctctcgcagg ccgtgcacga gaaccagcag gacgtcgtca tggcgccctg caacggctgc
    39181 tatcacaacc tgaagaaggc cgagcacgat ctcgcgcacg atcaggcctc gcgcgatgtg
    39241 accgagcgcc tgtcgagcaa ggcgggacat ccgacctaca aggccggtga ggccgagacg
    39301 atccacgcgc tcgactggct gaaggcgacc atcggcgagg agggcatcaa ggcgcgcacc
    39361 aaggggcggc tcgccggact cagaatcgcc aactactacg gctgcatgta tgtccgcccg
    39421 cgccacatct ttcccgagaa ggacaagggg ccgggctcgg aatcgacgca cgcgccgcac
    39481 ttcatggacg atctcctcgc cgcggccggc gcgacgaacg tctcattccc gctgaagacg
    39541 gcctgctgcg gcggcgccca cacgctgtcc gacagcgata cgtcgacgaa gctcgtcctc
    39601 aacatcctgc aggcggccga ggcttccggc gccgatgtca tcgccaccga atgcccgacc
    39661 tgccactcgg gcctcgagat gcaccaggtg cgcgccgaga agcgcttcgg caagaagacg
    39721 aacgtgaaga tgctctactt cacgcagctg ctcggcctgg cactcggcat cggtccgcgg
    39781 aagctcggca tccacacgaa catctcggat tcgcgcgatc tgatcatcga gaagcaactg
    39841 acctgacgcc tcgtcaggag attggcgtta cctgcagcgg catgccgccc ttcggccgca
    39901 gtgtcacgcg gctcacgggc tcgggcgtgt ggcggccgtc ccaaccgaag cgcgcgccgc
    39961 gaacgagcgt cgcgagcagc gctgtcgcct cgagcatggc aaaggtcgcg ccgatacaca
    40021 cgcgcgggcc gtagccgaac ggcatccact gcgtgcgcgc gtgccgggcc tcggcggcag
    40081 gcaggaagcg atcgggatcg aagcgcttgg ggtcttccca gagccggttg tggcggtgaa
    40141 tcgcaaagat cgggatgagc acaagcgtcg gagcatggag acggtgactg ccgagcgtgg
    40201 tcggctcgtc gatggtgcgc gccatcatgg cgacgggtgg atagatgcgc atggcctcct
    40261 tcaagacgcg gcgcgtgacc accagtcggt cgatgtgaga cgcctcgata ggcgcgcgac
    40321 ccgcgacttg tgcggcctcg gcccggatga gctcctgcca ggctggggca cgcgcgagga
    40381 gatagagcgt ccaggtcagc gccacggccg tcgtctcgtg gccggccatg tagaacgtga
    40441 ccatgttgtc gatgagctgc tcctcgctca tcggttcgcc cgtttcgggg tcccgggccg
    40501 acatcaggcg gccgaggaga tcgcggcgct cgccgccttc ggcgcgccgt cgtgagatga
    40561 ccatggcgac aatcgagcga aggtcgcgtg ccgcgcggcg catgcgtccg cggccaggat
    40621 gccagatgcc atccggcaga cggagttggc cggctgcgat ctcccacgtc accggtccca
    40681 gatagcgccg gccggcgttc tggatcaggt caccgacctc gaggccgtcg cctgagaaca
    40741 tcgtcgaggc gatgatcctg aaggtgaggt cgcccatggc gtgctcgacg gcgatcgtcc
    40801 gctcgcccgg ctttgccgcc caggccgcga cgatgcgctc gccttcggcc gccatcgcgg
    40861 ggacgagcgc ggtcagatcc tgcggccgga acaagggtgc cgccgtgcgc cgctgccagc
    40921 gccaggcggg cccgtccgag gtgagaagtc ccttgccgag ggccggcgac agcacacgct
    40981 tctcgaccga agtcttgtgg aagcgctcgg cgccaccgat caacacctcc tcgacaagct
    41041 ccggcccggt cacccagacg atgttggccc gaccgacacg gcgctccgcg aacggctcgt
    41101 cgaacacgat gcccggcagg ctggccggca ccgccgacnn nnnnnnnnnn nnnnnnnnnn
    41161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacggct gcggttcggc cccaacgccc
    41281 tcgggtacga caacggccat ggcaggcgcc gcccgtcgca gagcggccga cgatcaggtc
    41341 gccgtcgcgg cggccttggc cttgcgcgcg cgagcgatgc cggcggactt cttgaggcgc
    41401 gtgcgggcgc cggacgtgtt gccctggctg cgcgattgcg ccgggcgcgc ggccgtgcgc
    41461 cgctgcttct tcttcagctt ctcgacgttc cgcagcgagc gcttcgacat gacggcaatc
    41521 cttgcatgtg tggatgtgtg gtgacgcggc gcaacttggc cagcatcgca accgctgctg
    41581 taagggatcg gatgacgcga cgcaagcatg ctcgcnnnnn nnnnntgatg tatggccgga
    41641 cactggtccg actggccgga aacagccaca accgcacgat cctccaccga acgagcgttg
    41701 tgctcgcgcg gccttccacg ctaacattgc gccatgctcg gatcggacca caaattccgc
    41761 attcgcaagg gcgtgccggc cgatgccgca gcgctggcgg tgctgtttcg cgagacgtgg
    41821 gagctcacgt accgggcgat catcccggcc gtccatctcg agacgctgat ccgacggcgc
    41881 aatgaggatg cctggcggct cgcgcttggc gcgagcgagg gcatcctggt gctcgaggtc
    41941 ttgggcgtga ttgccgggta tgcgagcttc ggagccgcgc gcgggcgggg gcgatcggcc
    42001 taccgggggg agatctacga gctctatctg gctccggtct accagggtct cggcctcggc
    42061 gagcatctct tcgaggcgac gcgctctgca ctcgatcagc ggcggctgag cggcctcatc
    42121 gtctgggcgc tcgccgacaa cctgccggcg ctaagcttct attgggcgcg tggtgggcgc
    42181 ccgatcgcac gcgtcacgga tcggatggct ggcgtgccgt tgccgaaggt cgcctacggg
    42241 tggacctgat ggaggccgcg gctaccggct gtcaccgggc catagcatgc cctttgagcc
    42301 aaggcccatg ctcgagccgg gccacgttgg cgcgccaggc ggcgatccag gtgtcgcgac
    42361 cggtgaggtg cggcgtgggg ccgccgcgat gtcccgcggt cgcggaggtc gcttggccgc
    42421 gggtgcaggc gtggtcgtgg gcactcccca gggtgacgtc ggtggatcgg ccggctttgg
    42481 atcgacggtc gacgcggctg ccgtgggagc tggtgtgtcg ggatcgcccg acgcatcgcc
    42541 cggatggccg gtagcaattc gcttgcggct ttcctccgct tcggcggcat cggcgtaggc
    42601 cttgtggcgg gctgcctcag atgcggccca tggctccggc ttgcacttgc agttttcgac
    42661 gagcgtcttg cggtacttga aggcagtcgg gagcttcgtg taggcgcgcc cggtgagatc
    42721 gaccatctcg gcaggctcgg ccgtattcgg cagatagaat agctgcgcgt cctcgccgca
    42781 gctcgcgcga cagcggctgg cgtccttcca gaagttgctc cgcgaggtcg cgtagctgag
    42841 cggccagtag aagccatcac agagacgcac gcagaccgtc cgataggcgc cggatctcga
    42901 agatctgcga tcgtcatcgt cctcgcggcg cggcaaccaa gggtttggcg ccgagggagc
    42961 agcaggcggt cgatagttgg ccggcggagg ggcggnnnnn nnnnnccgaa aaccctccga
    43021 agagcttgtc caggaacccc tgagcatggg caggcggtgc cgacgacgtc gcgccgctgg
    43081 cgacgacggc agcggcagca agaccggcaa cgataccgcg gccaagagag cccacgcgcc
    43141 tcgacgcaac agaccacatg tacgcctacc cgcacaaaat cgaacgcggc ccgacacggc
    43201 catcgcgatc atttggcgtg aggggtgcaa atatttcgct aacgcggcgg ccgaattggc
    43261 acgacggccg cggcacgaag cagctcgcca ggacctcggc catgagctac tcggttgcgt
    43321 cgacgacgtc agcggccagg gagcccggat ggcccgccat atcgcaggac ca
//
LOCUS       DAORNH010000007        41814 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_705_length_41814_cov_2.624009, whole genome shotgun sequence.
ACCESSION   DAORNH010000007 DAORNH010000000
VERSION     DAORNH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 41814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41814
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_41814_cov_2.624009"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK264_01360"
     CDS             complement(<1..295)
                     /locus_tag="PK264_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74577.1"
                     /translation="MTHDAAGNTLTKSGGLPSIALAWDAENKLAVANVGADLVTVFLG
                     GGVEIDGAGTWTKYLHEEQVHLRECSGSLCQCSLTELERLRPILVCVDDCNEAS"
     gene            612..>842
                     /locus_tag="PK264_01365"
     CDS             612..>842
                     /locus_tag="PK264_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74578.1"
                     /translation="MCSLSPGIRAMAWLDISCPQSAASIAWNDPSVVAARHRDRRAGK
                     VLFAVGVEIGYEVREAPIAQLDRASASGAEGQR"
     assembly_gap    843..964
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1097..1492)
                     /locus_tag="PK264_01370"
     CDS             complement(1097..1492)
                     /locus_tag="PK264_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD74579.1"
                     /translation="MARTYKKVRYSPDRKDRGHTPASAAEGVEEALEVLEGRWKLVIL
                     FQLFGGKLVRFSELERAIPSISQKMLIQQLRQLESDGVVRRIVHHQVPPKVEYGLTDW
                     GEALCPALDELLKWAASREVFKGASSPEA"
     gene            1552..1881
                     /locus_tag="PK264_01375"
     CDS             1552..1881
                     /locus_tag="PK264_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD74580.1"
                     /translation="MSITLPATIDRFFVSENARDLSAIEQCFAADAVVRDEGKTITGV
                     AAIKAWRVETGEKYHHAVEPLSMTSLDAKVVVTGKVSGSFPGSPINLEHIFEIEGDWI
                     VSLEIRP"
     gene            1878..2657
                     /locus_tag="PK264_01380"
     CDS             1878..2657
                     /locus_tag="PK264_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD74581.1"
                     /translation="MSGDLQLNGFRALVTGGTKGVGEAVVARLREVGVKVLTTARSNI
                     ELSGNEMFVAADITSAEGCKAIADAVSGRLGGVDIVVHVVGGSSAPAGGFAVLDDEQW
                     QKELNLNLLAAVRLDRALLPAMIEQGSGVIVHVTSIQRQLPLPESTIAYAAAKAALSN
                     YSKALSKEVSPKGVRVVRVSPGWVETTAAKALVGRLAAEAGSDEQTARQRLMDSLGGI
                     PIGRPAKPREVADLIAFLASPLAASITGSEHVIDGGTIPTV"
     gene            3069..4691
                     /gene="ggt"
                     /locus_tag="PK264_01385"
     CDS             3069..4691
                     /gene="ggt"
                     /locus_tag="PK264_01385"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD74582.1"
                     /translation="MEIRTGRPVTLAPRGMVTSPSPLASAAGADVLRAGGSAVDAAIA
                     TAAVLSVVYPHMTGVGGDAFWLTWDAKARKARYLDGGGRAAASASLEQFTSKGLKELP
                     YRGVTPATLTTPGAVASWDAAHRAHGRLPLSRILDAAIGYARDGFPVTARLASWIEAT
                     KADLALSSEAAKIFMPGGAVPRAGAMLKNLNLARTLEAIASDGRSGFYEGAVGSELDR
                     FSQKSGGHFRASDLARQDARWGMPLETRYGDVTILNTPPPTQGFAVLEMLNLLEPLEL
                     HKKPMMGADWTHLLVQAKQIAYHDRDRWLADPKFSPVPTEMLVSKAYANERRRLIRPR
                     KALAWDEVPSYGSLKGDTTYLATVDGDGNAVSLIQSLYWGFGSAVVAGKTGVVLQNRS
                     AYFSLDPASPNRLEPGKTPLHTLIASLGLKGGKLWSVVGCMGADGQPQIQLQTYLAMI
                     EHGLDIQEALEAPRFLSGRFVLGEPRDTLHIEGRMPAATIAELERRGHSVNRWPDWCE
                     LAGHAHGITIDPETGMRAGGADPRSDGAAIGI"
     gene            4715..5887
                     /locus_tag="PK264_01390"
     CDS             4715..5887
                     /locus_tag="PK264_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD74583.1"
                     /translation="MRTEFDWSDPFLLDQQLSSDERTIRDTVARFAADRLAPLVTAWH
                     RDETFDPALHKEFASLGLLGGTLEGYGCPGLGHVAYGLAMRELERVDTAFRSAVGVQS
                     GLAMAAVHAFGSEAQKSRLLPAMAAGERIGCFGLTEPDHGSDPSGMAARARSAPGGGY
                     RLSGTKTWITQSPISDVLVVWAKDDDGRVGGFVIERGAAGLSTPKIEGKFGVRASPTG
                     MIVMDDVAVPASARLELVKGLAAPLSCLSRARFGISWGAMGAAEFCWHQARQYTLDRR
                     QFGRPLAANQLIQKKLADMQTEIALGLQAALRISRLAEEGLATPELFSLVKRNNAGKA
                     LEIARTARDMLGGNGISADYGIIRHLMNMEVVNTLEGTHDIHALVLGRSQTAIGAF"
     gene            5891..7117
                     /locus_tag="PK264_01395"
     CDS             5891..7117
                     /locus_tag="PK264_01395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD74584.1"
                     /translation="MSGASEKAATSTGFVLLGLVTAVQLLATFAVLSLPTLAPRAAPS
                     FGVGPEMIGVQISFIYFAASAFSNISGLIVRRYGAVLASTAALIVSGLGMIGVASGSL
                     VVAAIASLMIGVGYGLTNPAASHLLMKHGPRTRQNLVFALKQCGVPIGGVLAALLLPR
                     LAERIGWQWAIVVSSGLLGLLAIVMLPRRAQWDGDRDPSARISGGLLAGARFALANPT
                     LRSLSLMGGTFASFQFCLMSYVITMLVAEMKWDLVSAGVVASIMQAGGAVGRIAWSLI
                     ADRIGRGIEILMAIGILASLLAVMTACIGPGWSATAIVPLLAAFAFCLIGWNGVYMAE
                     VARTVGPVNVGIATGGVLVFNFAGVVLGPSVFGLLAKLTGSYALTYGIFSVVPLIGVT
                     ALWRVLRRRAGEAWGS"
     gene            complement(7140..8933)
                     /locus_tag="PK264_01400"
     CDS             complement(7140..8933)
                     /locus_tag="PK264_01400"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74585.1"
                     /translation="MAQGTFPPGGPTTKPVPKEPPKAVLPGTAAPVAVHYVIRGKGRI
                     RSTGAVVCDSETGKHLPSEGGVSCNGVAMAAGTMLRLTAEPRSGWRFKGWESQQGLCA
                     GVKQSACLAPARQALLVAVFEVDPGGSGAITIGKSTVSVHIPNAGGKVATAPFAPATI
                     ACTNASGKATGSCVVGVPKGGTIELTATPDGTHTFLGWSGTGLPASCQGSNPKCVLAP
                     TAPATTVTAMFGPLEATLAISEAPKAMSLSTGAPSKLVGISNEPRLFKGQLVRKGGPG
                     IEGESVELKLGDYGSCTAKTTINGAFACSILPKASPAEAAKRVGGNVIPEARLIATGK
                     PVPITGMKAILIVDQDGYTRDTLSALIVEAALKKAMPGGILLHNYTRNDLREEPERKH
                     LAMASRIYLTKELSETDAIKFDLPFMGSKPYFYFANNMLVRFGAPSVKGPANVTVGVS
                     MSGHNGSGPALFGFCYATETLDKPKCWAGADGAAPDLTGITLSGQIELKLVVNLDGRV
                     GITLASSTVKIDFDCGGLLSAVCGDVRDAMNYGLSKRIKDFVVNDILTSKVRVAFAQI
                     VAERGKISTLKAVEIDGGGNLIMRGTPKLAH"
     gene            complement(9414..11072)
                     /gene="ettA"
                     /locus_tag="PK264_01405"
     CDS             complement(9414..11072)
                     /gene="ettA"
                     /locus_tag="PK264_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HRD74586.1"
                     /translation="MANPQFVYHMHKVSKTYPGGKTVLKDISLSFFPGAKIGVVGLNG
                     AGKSTLLRIMSGELDDFVGEAWAQEGVKGGYLPQEPHLDPDKTVLENAMEGVAEKKAL
                     VDRYNEIAANYSDETAAEMAELQDKIDAQNLWDLDAQVEQALDALRCPPGESAVGNLS
                     GGEKRRVALTRLLLAKPDLLLLDEPTNHLDAESVAWLERYLKEYPGCIMLVTHDRYFL
                     DTITEWTLELDRGQGIPYKGNYSSWLEQKAKRLEVEKGQEEAKQRTLKRELEWIRSSP
                     KARQAKSKARIQAYDKLAEEAGREDVGKAQIKIAPGPRLGGVVVEANGLAKGFGDRLL
                     FEGLSFKLPPGGIVGVIGPNGAGKTTLFKLLTGQEKADAGTLRVGDSVQIGYVDQSRD
                     HLDDKKTVWEEISGGLDMVKLGEKIEVNSRAYCGWFNFKGPDQQKKVGMLSGGERNRV
                     HLAKMLKTGGNLLLLDEPTNDLDTETLSALESALEDFPGCAVVISHDRFFLDRLATHI
                     LAFEGDSHVEWFEGNFADYEEDKKRRLGDAAVEPHRIKYKRFER"
     gene            11176..11745
                     /locus_tag="PK264_01410"
     CDS             11176..11745
                     /locus_tag="PK264_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74587.1"
                     /translation="MEQTIHIHEDDWGLRNLYPLAAFDAAVADVAAAEKAGRDNVAPD
                     GIGWTSVHLIQAPKVDFTATGLAVDAVAPILERLLPWVRRFVATSMAGFSGGARDPYG
                     SYEDDAWCFGFGSHCFVKLEPAGVLVRQVWFEARTSDPYELGALRTAIEAIDGLAGAM
                     IADYVLEAVGRVRDRTFMERYLSSLADGA"
     gene            complement(11770..12648)
                     /locus_tag="PK264_01415"
     CDS             complement(11770..12648)
                     /locus_tag="PK264_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRD74588.1"
                     /translation="MIDFSDDAPILVFGGPYSNVRATRAMRRRALDLGIPSERTICTG
                     DVVAYCAEPAETVREIRDWGIHVVAGNCEESLANDLGDCGCNFDEGSACNRLAKSWFE
                     YASQRIGRADRDWMRALPTALRFSYAGLSFKVVHGGNAGISRWVFASMTDVLAEETAL
                     SASDITIAGHCGVPFVAEGHGCTWFNPGVVGMPANDGTPDAWYGLVSSTRDGVHLTTH
                     RLTYDHAAASRSLLAAGFADPYARALVSGLWPSLDVLPDVERAATGRALTDIDLRVTK
                     APRSGRQTGAPSVAAE"
     gene            complement(12711..13352)
                     /locus_tag="PK264_01420"
     CDS             complement(12711..13352)
                     /locus_tag="PK264_01420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD74589.1"
                     /translation="MVEVPSPSLQDGYTDVPPGKIAAVVTHLAMDADPGPRQAASRPE
                     LSIARIDQPRAGWYRDLFRRVGAEDWLWFSRLALDDAGLEAIIRNPSVEVYALRERDR
                     DEGLLELDFRDTGACEIAFFGVTGRLLGTGAGRHLMNFALATAWSRPIARLTVHTCTL
                     DHPGALAFYLRSGFKAVRRQIEIADDPRLTGLLAQSAATHVPIIRHDGIVKAR"
     gene            complement(13345..>13952)
                     /locus_tag="PK264_01425"
     CDS             complement(13345..>13952)
                     /locus_tag="PK264_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74590.1"
                     /translation="ILSRLVAEYQSYSRHYHTLEHVAEFIDHYRRLTPKPAAAHGIDD
                     ATFRLAIYFHDAIYDVTAKDNEEKSADWLRTQCGAQAVDDAVIAKACRYVMATKHLGG
                     GPRDLLPDEAYFADLDLAILGAPAERYRRYADDIRREYHQFDDASYANGRAGALRHLA
                     GVTPVFATIGRSLSVPSDWEVRARANIAAEIATLSSEAKRG"
     assembly_gap    13953..14063
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14064..15251)
                     /locus_tag="PK264_01430"
     CDS             complement(<14064..15251)
                     /locus_tag="PK264_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRD74591.1"
                     /translation="MALLAVDAAIERILGGVAPLTRDVVRLEQARGRVLAETLAARLT
                     QPPFDASAMDGFAVRASDVRKLPVRLAILGEAAAGHPFGGHVGPGEAVRIFTGAPVPD
                     GADAIVIQENATIAGSTVTVTEGVPEAANIRRRGYDFAAGSPLVSAGRRLGPRELALI
                     AAMGHGSVPVFRRPRVAVLSTGDELVLPGETPGPGQIVASNHLGVAGLAEQAGAEVRF
                     LGIAKDNATSIEHHVAEASDADILVTIGGASVGDHDLVAPVLERRGLKLDFWKIAMRP
                     GKPLMFGRLGDIRMLGLPGNPVSALICSRVFLVPLIRALQGMPNGGDGVTAVRAAVAL
                     EANGPRQHYMRAVVRRGGDGAAEVAPVRSQDSSLLSPLAEADALIVRPPGAAAVPAGG
                     IVSA"
     gene            complement(15256..15756)
                     /gene="moaC"
                     /locus_tag="PK264_01435"
     CDS             complement(15256..15756)
                     /gene="moaC"
                     /locus_tag="PK264_01435"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRD74592.1"
                     /translation="MSRLQISIGVPAHGAARMVDVSEKAVTQRTAIAEGFIAMAPETL
                     ALATSGKAKKGDVMATARIAGIMAAKRTHELIPLCHPLLLSKVNVVLEPSQSPVGIAV
                     TVEVGVSGQTGVEMEALTAASVACLAIYDMLKAVDRGMVIGPVRLVEKVGGRSGHWRA
                     DDAKGV"
     gene            complement(15753..16550)
                     /gene="trpC"
                     /locus_tag="PK264_01440"
     CDS             complement(15753..16550)
                     /gene="trpC"
                     /locus_tag="PK264_01440"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRD74593.1"
                     /translation="MSDILNKITEYKRAEISAAKAATPPAVMREHAKLAPPVRSFAGA
                     IEARLSAGETALIAEIKKASPSKGLIRADFDPPALARAYAEGGATCLSVLTDRPSFQG
                     APEYLIAAREASGLPVLRKDFMLDTYQVAEARSWGADCILIILAQVNDAVAADLAAAA
                     RDFGMDAIAEVHDEAELERAIKLGCRLVGINNRNLRTFETTLETCERLAPRLPRNRIV
                     IGESGIANNADVARLARSRIHTLLVGESLMRQPDVAAATRMLLRGPV"
     gene            16726..>17196
                     /locus_tag="PK264_01445"
     CDS             16726..>17196
                     /locus_tag="PK264_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD74594.1"
                     /translation="MTRSASNSVPPLRPFEAGTRGYFAVGAERMSKALNLGNMMRSAH
                     AFGAAFTFTIGARYQALEARADTSKGQQHLPHYEWPAVGDMRLPEGCELVGIELIEGA
                     VDLPEFRHPSRAAYVLGPEQGSLSPGLIARCDHVVKIPTRFCINVAMAGAIVMYD"
     assembly_gap    17197..17290
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17291..17781
                     /locus_tag="PK264_01450"
     CDS             <17291..17781
                     /locus_tag="PK264_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD74595.1"
                     /translation="STEAIMALEALLKKFTAAVEAGNGNALADCFTADGVYDDYFFGP
                     SKPGRAGIVEMLAHFYEGGKDFRWDFFDIAEGNPFAYASYRFSYTSTLPDAKGARVAF
                     GGISRLQMKGGLIAHYSEVFDRGMALAQLDFAPERLKKIALKYAAGVKARPEYARHAK
                     AS"
     gene            17879..>18520
                     /locus_tag="PK264_01455"
     CDS             17879..>18520
                     /locus_tag="PK264_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD74596.1"
                     /translation="MTTSAAPPPPHPTVKPLQGIRIIDVSSFLAGPFCSTQLAEFGAE
                     VWKLELPGVGDALRKFGTMTPSGDSLPWMQETRNKKVATLDLRKPEGAELLKRMIRKA
                     DVLVENFQPGTLEKWGLGWDTLKEENGKLIMVRISGFGQTGPYSPRPGFGRIGNAFGG
                     LSYLAGFPELPPTTPGSATIPDYLAGLYGALGVMFALRARDMTGRGQYIDIGLY"
     assembly_gap    18521..18530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18531..19124
                     /locus_tag="PK264_01460"
     CDS             <18531..19124
                     /locus_tag="PK264_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD74597.1"
                     /translation="EPVFRILDELAAAYQYKGYQRERMGPGTVNVCPHSHYPTKDGKW
                     IAIACTSDKIYSRLAEAQGQPELAAPGGRWEKIVDRDRERKDVDAWVETWTKKFTLAD
                     CIKECDKFQVPCGPVYSISDIFKDPHYAARENILRMNDPRVGEIAMPNVVPRLSDTPG
                     SVEWLGPPMNANTDELYKGLLGMSDAEIGRLKDKGVV"
     gene            complement(19121..19882)
                     /locus_tag="PK264_01465"
     CDS             complement(19121..19882)
                     /locus_tag="PK264_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74598.1"
                     /translation="MTALLSLRAISKRYGAVEALAGIDLDIHAGEVVAICGDNGAGKS
                     SLIKVMSGAEAPSAGSLVLDGVVQRFASPADALARGIATIYQHLALAPRLSIAENVFM
                     GAELTRTLLPGVAILDKRAMRASARAYLARLAISIDDMRRPVERLSGGQRQAVAIARA
                     LRWNARIVILDEPTAALGVKETAVVLGLVRKLTEEGRTVVLISHAMADVVAVASRVVI
                     LKLGSKIVDRQLGDLTAPALAEMIVAGRESVRKPG"
     gene            complement(19879..20289)
                     /locus_tag="PK264_01470"
     CDS             complement(19879..20289)
                     /locus_tag="PK264_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD74599.1"
                     /translation="MSQTITRGIQPMLDEARKVVDEIPPDDATKLHGRDDVVLVDLRD
                     PRELARDGKVPGAFSCPRGMLEFWIDPASPYHKPIFAEDKKFVFFCAGGLRSLLAAKT
                     AQDMGLKPVAHVIGGFKAWKEAGGPTEPGEPKKS"
     gene            complement(20422..21459)
                     /locus_tag="PK264_01475"
     CDS             complement(20422..21459)
                     /locus_tag="PK264_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD74600.1"
                     /translation="MTKSTLPICRVMAATAIAASLCSGPAAAQGEVNIYSYREPKLIE
                     PLLKAFTAKTGIRPKIIYASAGLNEKMAAEGALSPADLLFTVDVGRLAEAKDAGLTQP
                     VKSAILDTVPAAYRDAEGHWFGLTMRARVIYASKERVKQDTITYEELADPKWKGKICI
                     RSGQHVYNTSLVATMIAHAGESATETWLKGLRANLGRKPAGGDREAVRDVKAGICDIA
                     IANTYYMAAMLANPEQKAWAESVRIIFPNVTDRGSHVNISGVALARHAKNRDNAVKLM
                     EFLASDEGQRIYAEINYEYPISAKVPVSASVASWGTLKPDALPLVNITKFRKRASELI
                     DKVAFDAGPGN"
     gene            21902..22834
                     /gene="hemC"
                     /locus_tag="PK264_01480"
     CDS             21902..22834
                     /gene="hemC"
                     /locus_tag="PK264_01480"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRD74601.1"
                     /translation="MTQSRKIRVGTRGSPLALAQACQTRSRLMQAHGLPEEAFEIVVI
                     KTSGDRIQDRPLADLGGKGLFTKEIEEALIAGEIDVAVHSMKDVPTELPAGLGIVAYL
                     PRADVRDALITATASRIPELPAGAVVGTSSLRRQAQLRNLRPDLRIVPFRGNVETRLR
                     KLREGEAMATILAMAGLKRLGLDQHARAAIEPEEMLPAVAQGAIGIEARLDDAGMCAR
                     LAAIHHQPTGIELAAERAFLARLDGSCRTPIAALARLEGSRVTFRGEILAVDGGKVVR
                     ASRDGSASDAERLGRDAAEEVLRTAGPGFLSAGT"
     gene            22841..23554
                     /locus_tag="PK264_01485"
     CDS             22841..23554
                     /locus_tag="PK264_01485"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRD74602.1"
                     /translation="MRILVTRAAEDAAALATRLEALGHTPVIAPLLEVAYLPEALDLG
                     NAKGLIVTSRYALEALAREPAALAAACRRTIYCVGESTGQRARELGAPDVVAGPGTAS
                     RLIGMIRSQGVKADGPLLYLSAKEVAVDIAGELRLAGLDVRRQTVYRMVAVRTFKPAV
                     ARDLADGRIEAVILMSQRTARIYVELMSSAGLGVAARRVHHLCLSPSVAAALAPLGGT
                     LVAVAAHPTLDDLLTLIPG"
     gene            23703..25466
                     /locus_tag="PK264_01490"
     CDS             23703..25466
                     /locus_tag="PK264_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74603.1"
                     /translation="MADKKDQKPQAADSSGQKRPFATIDIKATEVKAEGKPVPPPATP
                     VSGGKPGDPARPADAKPGATGTPKPSDAKPGDAATAGPAIAPNVAAATPPPARGGFGR
                     LVSHTLAGIVGGVLALGGASQVAPSLGYPLFAPAGPVTPQIPDEVKSRLAAIEAAVAG
                     KAAASAVPADVAKRLAVGDAAAQRAEELAKALTALNATHARLAADTKALQEAVAKQAT
                     GAELAAKLAKLEETIASISAAAAADPQKAGRIPELVRIAGKLSDLEEALSTRLSALRK
                     DISLEIATRTQSVGEASEAAKAGATRLDKEVTAIRSDTVRVGQRADDLKVGVERLATL
                     LKGLQDDAATLRATVDTLKSDLDTRLKAVAKPADIAAAIAPVAAKVASLDTNVAGVVR
                     GEEDRRTNAERIVLSLELANLRRAMDRGKGYKAELAEVRRVAGTRLDLSVLERFQDKG
                     VPALADLGRDFNALIGPMLDIDAEPKGAGVVDRLLAGAKSVVRVRRTSAPPEDKSAEG
                     IIARMETALRENRVADVLAEAKGLEARVAAPAQDWLKKVEARHAVDGALAAIDNALKA
                     ALGAKTAAPAAATPADKGAKQ"
     gene            25463..>26371
                     /locus_tag="PK264_01495"
     CDS             25463..>26371
                     /locus_tag="PK264_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HRD74604.1"
                     /translation="MIRVILFLILCGVIASGLVWLAERPGTLAIRWQDRALDISLFRA
                     VVILSLLIAFAVMAWALLRYVWQSPAAVGQFFMKRRQQKGLDALSSGMIAVGAGDRQL
                     ALRYAQQARKSLPNEPLTHLLRAQAAQLSGDRGTSRRIFEAMLSSPDTEQLGLRGLYL
                     EAEKEGEREAARQFAERAMVLNPRLPWPTEALFELQCRDSDWEGALSTLSSARTNTLI
                     DKPTADRRRAVLLTAQAQAIEEDESEKSLSLALEAHRLAPDLVPAAAISARVLASRGN
                     PARVAKIVETTWQRAPHPELATAYAYS"
     assembly_gap    26372..26414
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26415..>26739
                     /locus_tag="PK264_01500"
     CDS             <26415..>26739
                     /locus_tag="PK264_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD74605.1"
                     /translation="VRLAPHSIEAPIALATAALEAKSFDEARRVLEALSGERQTQRVC
                     SLMARIEAEDGENRGKAREWLGRAANAPRDPAWTADGVVSDRWAPISPATGRLDAFEW
                     RVPSTS"
     assembly_gap    26740..26966
                     /estimated_length=227
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26967..27168
                     /locus_tag="PK264_01505"
     CDS             <26967..27168
                     /locus_tag="PK264_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74606.1"
                     /translation="MPARVVMPAGTGAARSAPEASGEGKKVIEIRLDGDDPKVDFPER
                     DSSDPGSARDKTGARTSGSGKA"
     gene            27274..28473
                     /locus_tag="PK264_01510"
     CDS             27274..28473
                     /locus_tag="PK264_01510"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRD74607.1"
                     /translation="MHLDKLKPASGADVRGRRVIVRADLNVPVRDGRVTDATRLERLL
                     PGIRDLSKRGARVIVISHFGRPKSGPDPELSLRPIAAAMQAALGRPVAFGADCIGAAA
                     GSVAAGMKDGDVALLENLRFHRGEEKNDPAFAAELAKLGEVFVGDAFSCAHRAHASTD
                     AVARLLPSYAGPLLMEEITALRRALEKPERPTAAVVGGAKVSTKIPVLKNLVAKVDKL
                     IIGGGMANTFLQAQGIEVGKSLSEPEFHETAREITAAAKARGAEIVLPVDGVVAREFK
                     ADAASSVVLVTATPADAMILDVGPKSVAHLMSVIAACRTILWNGPLGAFEIAPFGEGT
                     FALAREAARLTKAGKLVSVAGGGDTVAALNAAGVTDEFTYVSTAGGAFLEWLEGRDLP
                     GIVALTR"
     gene            28470..29192
                     /locus_tag="PK264_01515"
     CDS             28470..29192
                     /locus_tag="PK264_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRD74608.1"
                     /translation="MTRTIPIAIDGPLPAAVIFDLDGTLVDSAADIADALNTGLAVIG
                     VPPFPVEAVHGMIGGGGRVAIARALDAAGRTASEDELARIFSGFMARYRVVSSEGRGL
                     YSGAHEVLGGLRTRGVKLALCTNKADDVARIALRALGIADYFHAAIGAMDGKPRKPEP
                     GQIHWLLDELGVAARDAVLVGDSAADAGAARSAGLGGLILTSFGYSRVPVAELAADAV
                     IDHLAELPRALARWAAPRNVRG"
     gene            29210..30616
                     /locus_tag="PK264_01520"
     CDS             29210..30616
                     /locus_tag="PK264_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRD74609.1"
                     /translation="MSSMKAAKGPTETLGDFVANLTFEQLPGPVVAQACNVIADAVGC
                     AIGAAADDPEKARIVRTLVTSFASRPEAVIWGSDVAADPALAAFGNGILVNATDFDDT
                     HKRALLHTGSVVVPPALALGQALHLDGRRIVTAVVAGYETAVRVGMAVMPTHYRYWHS
                     TATNGTFGAAAAAASAMSLDPAGARTALGHAGTQAAGLNTFFTSGDFTKSLHPGKAAF
                     NGVLSARLARIGATSPPTVLEHEKGYLSAFSAEPKAAALTAGLGKTWEILQNGFKFFP
                     SILASHSAIQATLDAVREARPDPRAIVSIRNETYETVRTHFSAKAVTTEMGARVSVPY
                     CVAVAALDGTVGQAQFTTERVNRTDVQALLARTETIADPALTTLYPAKFPARVTITLA
                     DGRTIVRERAYPKGDPQEPLTPAEIEAKFLANAERGLGATAARELLSLIRALPDEREF
                     SRLGKLLCPRPAPSRASR"
     gene            30690..31538
                     /locus_tag="PK264_01525"
     CDS             30690..31538
                     /locus_tag="PK264_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD74610.1"
                     /translation="MKLVRFGAPGAEKPGLVDAGGVIRDLSQHVKDITGDTLSQAALD
                     KLRKLDPKSLPEAPKGVRLGAPVGGVRNFVAIGLNYTDHAKEVGAPIPAEPIIFYKLA
                     NCIVGCNDDVMIPRASTKLDWELEIAFVVGKRARYVELKDAAAHIAGYTICNDISERN
                     FQLERGGQWMKGKSAETFGPLGPWLVTSDEVKDVNNLPMYLDVNGKRMQTGSTATMIF
                     KCDFILHYVSQFMVLEPGDVVTTGTPPGVGAGMKPPTFLKAGDVMELSIEGLGRQKQK
                     VVPFKL"
     gene            31582..32400
                     /locus_tag="PK264_01530"
     CDS             31582..32400
                     /locus_tag="PK264_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD74611.1"
                     /translation="MPLIDIDRVSAIVREVGQRVVLPRWRNLGERDVVEKSGPGDLVT
                     VADKAAEEALTPALSALVAGSVVVGEEAVHADPTRRGLFDGDGPVWVIDPIDGTSAFA
                     SGKPEFFVMVALVSGRELAAGWIYGPASDTLYAAERGAGAFRVHGDGPHERIVRVVPP
                     AELSAFRGAIGRRYLSAEEQAAMAARLPRVGPCETALYAGREYPRLVSGELDFLLYRR
                     TEPWDHLPGLAIASEQGFAYCRHDGSAYLPGMNSGGLVIAPELWLERVRANFFA"
     gene            complement(32950..33210)
                     /locus_tag="PK264_01535"
     CDS             complement(32950..33210)
                     /locus_tag="PK264_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74612.1"
                     /translation="MKTIISAIAAAAVVAISGPASAKGVFETLAESAPKHPIFAELDR
                     VAPRSDVFETLNQTAPRADIFDTLNQTAPKQPVFIWIDRNAP"
     assembly_gap    33491..33500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33636..34526
                     /locus_tag="PK264_01540"
     CDS             33636..34526
                     /locus_tag="PK264_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HRD74613.1"
                     /translation="MPISAGTDRRPNESGALRSLCSRLGGALIAVSVIAGTATAARAE
                     SIEATFARYAKGSAAAVDHGVWARLLTEHVKPGADGLNRVDYKAFKAKGHTDLKAYIG
                     SLVVVDVAKLDRPEQFAFWSNLYNAKTIDIVLDKYPVTSIKDISLGGGILASITGGPW
                     KAKVVKVMGHDLSLDDIEHVILRAIFKDPRVHYAVNCASVGCPNLMTTAFTGAMLEAQ
                     LEAGAKAYVNHPRGIRVEGGRVYASNIYDWFKADFGGSADGVLKHVRVYADPALKQKL
                     EGLTRIYDYGYDWSLNDVAR"
     gene            34549..35334
                     /locus_tag="PK264_01545"
     CDS             34549..35334
                     /locus_tag="PK264_01545"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRD74614.1"
                     /translation="MAPQTSVPTGSFRRLLPAVALIAVMAVAYWRGWHQYLSFKTIGL
                     NYELLKGFIESNLLGALAIFVAAYIAVVALSLPGALIMSLSGGLLFGWKLGTPAIVLG
                     ATTGATILFLIANTSLGEPLARRAGPALTKLREGFRANAMSYLLFLRLVPAFPFVVIN
                     LAAALLGVPLRTFIFGTALGIIPGSLAYATAGSGLGSVVEAQNAVHKACLAKTPANPD
                     VACPYSIDTSALITKELIAAFILLSVVALIPVAIEKWRARHAA"
     gene            35324..36808
                     /locus_tag="PK264_01550"
     CDS             35324..36808
                     /locus_tag="PK264_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD74615.1"
                     /translation="MQRKATPDAPAVPPGARSYSEEIKADLCVIGAGSGGLSIAAAAS
                     QLGVSVVLIEKHKMGGDCLNYGCVPSKALIAASRRAHAMRSSAPFGIAPVVPAIDPRA
                     VHDHVKGVIAAIAPNDSVERFTGLGVRVIQAAARFLDKSTVIAGDTAVKARRFVIATG
                     SSPVVPPIPGLVNVPYFTNETVFDNQQKIDHLIVIGGGPIGMELAQAHLRLGARVTVL
                     EGLKALGKDDPELTDVVLKSLRAEGLVIREGARVEMLERGAGPAAVRVTIATGEAKEI
                     VEGSHLLIATGRKPNLADLNLDAAGVKFDKRGIQVDKGLVTSNSKVFAIGDVVGGLQF
                     THVANYHAGIVFRRALFRLPAKTRDDIVPWVTYTDPELAHVGLAEDAARKAHGKINVY
                     RFPFHDNDRAQAERATHGFIKVVTSAKGKILGASIVGEHAGELIQMWSLALSQGMNIK
                     QMTQWISPYPTLSEVSKRAALGYYAAQAGSPLARKVVGWLRVFG"
     gene            36925..38445
                     /locus_tag="PK264_01555"
     CDS             36925..38445
                     /locus_tag="PK264_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD74616.1"
                     /translation="MDARVERGGSEDAGAASAVRRGVLAGVVDRLRRPGLSTKLLRLT
                     VLFVMLAEVLIFVPSIAKFRLDWLNDRLKSAHLASLALEAVPGGQVPATLRKELLEGA
                     RVRAVAVRRADQRLLVLPADGPIIVDRTYNLAEMQPAGSTSPVVSGRRIEAAYDALIT
                     YFLASETYIRIIGHSGMAASPERDVIDVVLPVGPLKSAMIQFGLNILALSIVISIITA
                     GLIFLVLDRLLVRPIMNIADNVVRFAANPEDQQAFIKPSGRNDEIGTAEHEIRRMQQE
                     LSQVIHQKSRLAALGLAMSKINHDLRNMLANAQLISDRLTTLPDPTVQQFAPKLIASL
                     DRAINFANSTLHYGRAEEAVPKRERLALKEFAREVGEGLGLPRAQVAFEVEMPDDLVI
                     DADPDQLFRILTNIMRNSIQAIEAEAQVRNRIVIGGRREGPMVTLSIADDGPGVPAKA
                     RAHLFQAFQGSTRKGGTGLGLAIVMELVAAHGGRISLRDDVPKGATFDIEIPDRAG"
     gene            complement(38454..39596)
                     /gene="aroB"
                     /locus_tag="PK264_01560"
     CDS             complement(38454..39596)
                     /gene="aroB"
                     /locus_tag="PK264_01560"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRD74617.1"
                     /translation="MTSPSPAGPVRKVHVALGARSYEVLIGGGLIEAAGCEIAARFPR
                     AAVAIVTDETVAAHHLARLERALAGSVTVRGALALPPGERSKCFTELARLSEWLLERG
                     VERGDLVIALGGGVIGDLAGFAAAIVRRGVRFVQIPTTLLAQVDSSVGGKTGINTPQG
                     KNLAGAFHQPSLVLADTDVLTTLPQRELKAGYAEVAKYGLLGDAAFFAWLEAHHARVL
                     SHDRDALTHAIETSVAMKAAIVARDETETGDRMLLNLGHTFGHALEAWAGFSSRLLHG
                     EAVAIGMAEAFRYSERCGIAPMAAARRVMQHLRAVGLPTEIAHIQGNSLPSVDELMAL
                     MSQDKKVQRGQLTFILARGIGEAFVTRDVDRADLVAFMTEEIARPA"
     gene            complement(39593..40202)
                     /locus_tag="PK264_01565"
                     /pseudo
     CDS             complement(39593..40202)
                     /locus_tag="PK264_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
     gene            40420..40563
                     /locus_tag="PK264_01570"
     CDS             40420..40563
                     /locus_tag="PK264_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74618.1"
                     /translation="MPSLVRFFVVVGLLSAVTFGGLYIMSEYFEPDQREVTRPVPGVK
                     VRK"
     gene            41116..>41814
                     /locus_tag="PK264_01575"
     CDS             41116..>41814
                     /locus_tag="PK264_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74619.1"
                     /translation="MAPTTARGGLPPTLWEGADGPTIERLVGAIPDPGRSAALQALWR
                     QVWTHRAPAPEGAKQFEAIRFEAFWRAGLVGEIVARAKAVDASDPLVALMLARAEIAL
                     GERETGCARARPVAAHMKSLVPALAAEALVMSSYCATSAKGRGAAELAAGLLRESKVD
                     APIPLAVLDALSGSVRGSPQLPRVVRAIDYRFLELGGTVDAAALVERAEPALLAILAE
                     LPGDPRGRVLAAEAA"
BASE COUNT         6663 a        13709 c        13882 g         6943 t
ORIGIN      
        1 cagacgcttc gttgcaatca tccacgcaga ccagaattgg ccgcagtcgc tcaagttccg
       61 tcagcgagca ctggcacagc gagccgctgc actcccggag gtgtacctgc tcttcatgga
      121 ggtacttggt ccacgtgccg gcgccgtcga tctcgacgcc tccgccgagg aacacggtga
      181 cgaggtccgc gccgacattt gcgacggcga gcttgttctc cgcgtcccag gcgagcgcga
      241 tggatggcag cccgcccgac ttggtgagcg tgttgcctgc cgcgtcatgg gtcatcgcga
      301 acgatcacgt cgacggcagc ggccaggtct agcggcgccg cccctcgagc acgccgccag
      361 ggacttgtcc ctggacccaa aattggtttc caaaggccaa agccattggc ggggttcggg
      421 gccgcgcccc gatggctaat ggcgggacgt gaccggcgtc gtacgtgaac gtctgcgtcg
      481 aggctgcgtt gccggcgttg gtcgcggtca gcagctgtca gcgggccgcc ccgccgagat
      541 gacggcggtg atgcgccccc tggcgtcgcg cccgaaagtg atgtcccgga ggacgcccgc
      601 gcccttggag catgtgcagc ctctcgcccg gcatccgggc gatggcctgg ctggacatca
      661 gttgcccaca gagcgcggca tccattgcct ggaacgatcc gagcgtggtc gccgcgaggc
      721 atcgggatcg ccgtgctgga aaagtgctgt ttgctgtggg ggtggagatc ggctatgagg
      781 tgcgcgaggc gcccatagct cagctggata gagcgtcggc ctccggagcc gaaggtcaga
      841 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnngctaaa tcaatccgtt atctcgctat tgtgatccta ggttttcgta acgcgcggga
     1021 tcactttgca ccggcagcag cttgcctgac gatgaataga gcgtcgaaat tgttccagag
     1081 cagctcgacc agaacctcag gcttcggggc ttgaagcgcc cttgaagacc tcacgcgatg
     1141 cagcccattt gagcaactcg tcgagcgccg ggcagagtgc ctcgccccaa tcggtcagac
     1201 cgtattcgac tttgggcggc acttggtgat gcacgatccg gcgaacaaca ccatcgctct
     1261 ccaactgccg cagttgctgg atcagcatct tctgggaaat cgacgggatg gcccgctcaa
     1321 gctcagagaa gcgcacgagc ttgccgccga acaactggaa aagaatgaca agcttccagc
     1381 gcccttcgag cacttcaagt gcctcttcaa cgccctcggc cgcggacgct ggcgtgtggc
     1441 cgcgatcctt acgatcaggt gagtacctta cttttttgta tgttcttgcc attaggcgac
     1501 catatgcaca tcttcgatct cgagcaaatg cagcgcaaag gagcaaacgc catgagcatc
     1561 acgctgccgg cgaccatcga tcgctttttc gtgtcggaga atgcgcgtga cctatcggcg
     1621 attgagcagt gcttcgctgc tgacgccgtc gtgcgcgacg agggaaagac catcactgga
     1681 gtggcggcca tcaaagcgtg gcgggtcgaa accggcgaga aatatcatca cgctgtcgaa
     1741 ccactcagta tgacgtcact ggacgcgaaa gtggtggtga cgggcaaggt gtcgggcagt
     1801 ttcccgggaa gcccaattaa tctggagcac atctttgaaa tcgaaggtga ctggatcgtt
     1861 tcactggaga tccggccatg agcggagatc tccaacttaa tggctttcgc gcgctcgtta
     1921 ccggcggcac caagggtgtt ggtgaagccg ttgttgctcg gctaagagag gttggtgtca
     1981 aagtcctgac cacggcgcga tcgaatatcg agctttctgg caacgagatg tttgttgccg
     2041 ccgacatcac cagcgccgaa ggctgcaagg caattgccga tgcggtcagc gggcgccttg
     2101 gaggcgttga catcgttgtc cacgtcgtcg gcggctcatc cgcgccggct ggcgggttcg
     2161 cggtgctcga cgatgagcag tggcaaaagg agctcaacct caatttgctg gcggccgtgc
     2221 ggcttgatcg agcgctcttg ccggcaatga tcgagcaagg ctctggggtg atcgttcatg
     2281 tcacatcgat ccagcgccag ttgccgctac ccgagtcgac catcgcctat gccgcagcaa
     2341 aggctgcact atcgaactac agcaaggctc tttccaaaga ggtgagcccg aaaggggtgc
     2401 gcgtggtgcg ggtatcgccc ggttgggtgg agacaaccgc ggccaaggca ctcgttggcc
     2461 gccttgcggc ggaggccggc agcgacgagc agaccgctcg tcaacgactt atggattcgc
     2521 tcggcggcat tccgatcggc cgacccgcca agccccgcga ggttgccgat ctgatcgcgt
     2581 tcctcgcgtc acctcttgct gcctcgatca ctggcagcga acatgtgatc gatggcggca
     2641 ccattccaac cgtttaagta tcatcgttgc cgcttcaaag ctgctgttga gattacaatt
     2701 tgatttcaat cacataggtg ttcgcacgtt tattcagcac ggtgcgccaa tgagttaggg
     2761 cctactgatt gcgaatctgg atcgcccata ggtaacgcat tggcaacatt tttgccggtg
     2821 cttggcgggg tggtatctgg ggggactgag gcgtcgacct gttgcctccg cgcgaagctt
     2881 ccaccgcctt cggcatcctt cggcggggcg gggctgctcc ctgccgcggc cctcggagcg
     2941 atcgccgtgc cactcgcctg tctcgccgtc gcgcgccgca agcctgatcc gcggcaatga
     3001 cggcgtgacg cctcggcgcc actcatggca tgagcgacgg gcccattgag caattccgga
     3061 gctgtggcat ggaaatccga accgggcgcc ccgtcaccct ggcgccccgc ggcatggtga
     3121 cgtcgccgag cccgctcgcc tcggctgccg gtgccgacgt cctgcgcgcg ggcggctcgg
     3181 ccgtcgatgc ggccattgca accgcggccg tgctctcggt cgtctacccg cacatgacgg
     3241 gcgtcggcgg cgatgccttt tggctgacct gggacgccaa ggctcgtaag gcgcgctatc
     3301 tcgatggcgg cggacgggcc gcggcgagcg cgagcctcga gcaattcacg agcaagggcc
     3361 tgaaggagct gccctatcgt ggcgtcacgc cggcgacgtt gaccacccct ggcgcggtcg
     3421 cgagctggga tgcggcgcac cgcgcacacg ggcggctccc gctctcacgc attctcgatg
     3481 ccgcgatcgg ttacgcccgc gacggcttcc ccgtgaccgc gcgcctcgca agctggatcg
     3541 aggcgaccaa agccgatctc gccctttcga gcgaggccgc aaagatcttc atgcccggtg
     3601 gcgcggtgcc gcgcgctggc gccatgctca agaatctgaa cctcgcgcgc accctcgagg
     3661 cgatcgcatc ggacggccgg agcggcttct acgagggcgc cgtcgggtct gagctcgatc
     3721 gcttctcgca gaagtctggc ggacactttc gtgcgagcga ccttgcccgc caggacgctc
     3781 gctggggtat gccgctcgag acccgctatg gcgatgtgac gatcctcaat acgccaccgc
     3841 cgacgcaggg attcgccgtg ctcgagatgc tgaacctcct cgagccgctc gaactccaca
     3901 agaagccgat gatgggtgcc gactggacgc atctcctcgt ccaagcgaag cagatcgcct
     3961 atcacgatcg cgatcgctgg ctcgccgatc cgaagttcag ccccgtgccg accgagatgc
     4021 tcgtctcgaa agcgtatgcg aacgagcgcc ggcgcctcat ccgcccgagg aaggcgctcg
     4081 cctgggacga ggtgccgtcg tatggaagcc tcaagggcga cacgacgtat cttgcgaccg
     4141 tcgacggcga cggcaacgcc gtctcgctca tccagagcct ctactggggt ttcggctcgg
     4201 ccgtcgtcgc cggcaagacc ggggtcgtat tgcagaaccg atcggcctat ttctcgctcg
     4261 atccggcgag ccccaatcgc ctcgagcccg gcaagacgcc gctgcacacg ctgatcgcat
     4321 cgctcggctt gaagggcggc aagctgtggt cggtcgtcgg ctgcatgggc gccgacggcc
     4381 agccgcagat ccagctgcag acctacctcg ccatgatcga gcacggcctc gacatccagg
     4441 aggcgctcga ggcgccgcgc ttcctatccg ggcggttcgt gctcggcgag ccgcgcgaca
     4501 cgctccatat cgaagggcgc atgccggccg cgaccattgc cgagctcgag cggcgcggcc
     4561 acagcgtcaa tcgctggccc gattggtgcg agcttgcggg ccacgcgcac ggcattacca
     4621 tcgatcccga gacgggtatg cgcgccggcg gcgccgatcc gcggagtgat ggcgcggcga
     4681 tcggcatatg agccgtgaag ctgggagaca aggcatgcgc acggaattcg attggtccga
     4741 tcccttcctc ctcgaccagc agctctcgag cgacgagcgg acgatccgcg atacggttgc
     4801 gcgcttcgcc gccgatcggc tggcaccact tgtcaccgcc tggcatcgcg acgagacatt
     4861 cgatccggcg ctgcacaagg agttcgcgag cctcggtctt ctcggcggca ccctcgaagg
     4921 ctacggttgt ccgggcctcg gccacgtcgc ctacgggctc gccatgcgcg aactcgagcg
     4981 cgtcgatacc gcgtttcgta gcgccgtcgg tgtgcagtca gggctcgcaa tggcagcggt
     5041 tcacgccttc ggcagcgagg cgcagaagtc gcggctcctg ccggccatgg ccgcgggcga
     5101 gcgcatcggc tgcttcggcc tcaccgagcc cgaccatggc tccgacccct cggggatggc
     5161 cgcgcgggcg cggagcgctc ccggcggcgg ctaccggctc tcaggcacca agacctggat
     5221 cacgcagtca ccgatctcag atgtgctcgt cgtgtgggca aaggacgacg acggtcgcgt
     5281 cggcggcttc gtcatcgagc gcggagccgc gggcctttcg acgccgaaga tcgagggcaa
     5341 gttcggcgtc cgcgcctcgc cgacgggcat gatcgtcatg gacgacgtcg cggtgccggc
     5401 ctctgcccgc ctcgagctgg tgaagggtct cgccgcgccg ctctcctgcc tgtcgcgcgc
     5461 gcgcttcggc atcagctggg gcgccatggg tgctgccgag ttctgctggc accaagcacg
     5521 ccagtacacg ctcgaccgcc gccagttcgg ccgcccgctc gccgccaacc agctgatcca
     5581 gaagaagctc gccgacatgc agaccgagat cgcgcttggc ctgcaggcgg ccctgcgcat
     5641 ctcgcgcctc gccgaggagg gcctcgcgac gcccgagctt ttctcactgg tcaagcgcaa
     5701 caacgcgggg aaggcgctcg agatcgcgcg gaccgcgcgc gacatgctcg gcggcaacgg
     5761 catctcggcc gactacggca tcatccggca cctgatgaac atggaggtcg tcaacacgct
     5821 cgaaggcacg cacgacatcc acgccctcgt gctcggccgc tcgcagacgg ccatcggggc
     5881 gttctgagca atgagtgggg cgagcgaaaa agcggcgacc tcgaccggct tcgtgctgct
     5941 cggcctcgtg accgcggtgc agctgctcgc gacgtttgcc gtgctctcgc ttccgacatt
     6001 ggcgccgcgc gccgcgccgt ccttcggcgt cggcccggag atgatcggcg tgcagatcag
     6061 tttcatctac ttcgcggcat ccgccttctc taacatctcg ggcctgatcg tgcgccgcta
     6121 cggcgcggtg ctcgcgagca cggcggcact cattgtctcg ggcctcggca tgatcggggt
     6181 ggcgagtggc tcgctcgtcg ttgcagccat cgcctcgctg atgatcggcg tcggctacgg
     6241 actcacgaac ccggcggcgt cgcatctcct catgaagcat gggccacgca cgcggcagaa
     6301 cctcgtcttt gcgctgaagc agtgtggcgt gccgatcggc ggcgtgctcg cggccctgct
     6361 cctgcctcgc ttggccgagc gcatcggctg gcaatgggcg attgtcgtga gttccggcct
     6421 gctcgggctg ctcgcgatcg tcatgctgcc gcggcgcgcg cagtgggatg gcgaccgtga
     6481 tccctcggct cgcatcagcg gcggcctcct ggcaggcgcc cgcttcgcgc tcgccaatcc
     6541 gacgctgcgc tcgctctcgc tgatgggcgg caccttcgcc tcgttccagt tctgcctgat
     6601 gtcctacgtg atcaccatgc tcgttgccga gatgaagtgg gacctcgtct cggcgggcgt
     6661 cgtcgcttcg atcatgcagg ccggcggcgc cgtcggacgc atcgcctgga gcctcatcgc
     6721 cgaccggatc ggccgcggca tcgagatcct gatggcaatc ggtatcctcg cctcgctgct
     6781 cgccgtcatg acggcatgca tcggtccggg gtggtcggcg acagcgatcg taccgctact
     6841 cgcggcattc gcgttctgcc tgatcggctg gaacggcgtc tacatggcgg aggttgcgcg
     6901 gacggtgggc ccggtcaacg tcggtatcgc gaccggcggc gtcctcgtgt tcaatttcgc
     6961 tggcgtcgtc ctcggtccga gtgtcttcgg cctgctcgca aagctcaccg gcagctacgc
     7021 tctgacctac gggattttct ccgtcgtccc gctcatcggc gtcaccgcgc tctggcgcgt
     7081 gctccgacgg cgcgcgggcg aggcgtgggg aagctgagcc cgcccgcgcc cggagcacct
     7141 cagtgcgcga gcttcggcgt cccgcgcatg atcaaattcc cgccgccgtc gatctcgacc
     7201 gccttgagcg tggagatctt gccgcgctcg gcgacgattt gagcgaacgc gacccgcacc
     7261 ttgcttgtga ggatatcgtt gacgacaaaa tccttgatcc gcttactcag gccgtagttc
     7321 atagcgtccc gaacgtcgcc gcacaccgcc gacagaagcc cgccgcagtc gaagtcgatc
     7381 ttcacggtcg agctggcgag cgtgataccg acccgcccgt ccagattgac gacgagcttc
     7441 agctcgatct gccccgagag cgtgatgccg gtgagatcgg gcgccgcgcc gtcggcgccg
     7501 gcccaacact tcggcttgtc gagcgtttct gtcgcgtagc agaagccgaa gagggcgggc
     7561 ccgctgccgt tgtggccgga catcgagacc ccgaccgtaa cgttggccgg gccctttacg
     7621 cttggcgccc cgaaccgaac gagcatgttg ttcgcaaagt agaagtacgg cttgctgccc
     7681 atgaacggca ggtcgaactt gatcgcgtcc gtctccgaga gctccttagt caggtagatc
     7741 cgcgacgcca tcgccaagtg cttgcgctcc ggctcctcgc gcagatcgtt cctcgtatag
     7801 ttgtgcaaca ggatgccccc cggcatggcc ttcttgagtg ccgcctcgac gatgagcgcg
     7861 gagagcgtgt cacgcgtata gccgtcctgg tcgacgatca atattgcctt cattcccgtg
     7921 atcgggaccg gcttgcccgt cgcaatcagc cgcgcctcgg ggatgacgtt gccgccaacg
     7981 cgcttggcgg cttcggccgg tgacgccttt gggagaatgg aacaggcaaa cgcgccgttg
     8041 atggtcgtct ttgccgtgca cgatccgtag tcaccgagct taagctcgac cgactcgccc
     8101 tcgatccccg gaccgccctt gcgaaccagc tggcccttga agagccttgg ctcgtttgag
     8161 atgccgacga gcttcgacgg agctcccgtc gagagcgaca tggcctttgg tgcctcggag
     8221 attgcgagcg tcgcctcgag cggcccgaac atggctgtga cggtcgtcgc cggcgccgtc
     8281 ggcgcgagga cgcatttggg attgctgccc tgacaggagg cggggaggcc cgtccccgac
     8341 catccgagga aggtgtgtgt gccgtccggc gtggcggtaa gctcgatcgt gccacccttg
     8401 gggaccccga ccacgcagct cccggttgcc ttgcccgacg cgttcgtgca ggcgatcgtc
     8461 gctggcgcaa acggggccgt ggcgaccttg ccgccggcat tcgggatgtg gaccgagacg
     8521 gtcgacttgc cgatcgtgat cgccccggag ccgcccggat cgacctcgaa gacggcgacg
     8581 agcagggcct gcctggcagg cgcgagacag gcgctttgct tcacgccggc acagagcccc
     8641 tgctggcttt cccacccctt gaatcgccat ccgctcctgg gctcggcagt caatctcaac
     8701 atcgtgccgg cggccatggc cacgccgttg caggagaccc ctccctccga cggcaagtgt
     8761 ttgcccgtct cggaatcgca gacgacagcg ccagtgctcc tgatccggcc cttgccccgt
     8821 atgacgtagt ggaccgctac cggggcagcc gttcctggca ggacggcctt gggcggctcc
     8881 ttcgggaccg gcttcgtcgt cgggcctcct ggtggaaaag ttccctgggc catcagaggg
     8941 gtagctccgg caatgccgat gacggccgcg accgctaaac ttctgcagat cgacatgagt
     9001 tgtattccct cgacagttgg catggagcaa cagacctcga tgccctcgag gagttgcttg
     9061 cgctcactct gacgctttgg gcgcgaaatt ggttcgatgc cctgcaattc gtggccacgc
     9121 gcattcgtag catgatgagg tcatgccgga gagggaccaa gcattggtca attcgatgtt
     9181 tgcgccaagg ttgcaattcc ggcagcagca gtcgcctcga gcatgcaggt cggcggcatg
     9241 tccagcgtca tgccgaaaac cagcgcgtgg gattgcagcg agcgatctcg atacgctcgt
     9301 gctgttccga ccacgtgtga tggccatgca gccgcctccc tcctcgcagt gacgaaggtc
     9361 gtgttcatat cgtgatcaag gtgccccggg ctcaaacgcg agagcgatcg ccctcaccgc
     9421 tcgaagcgtt tgtacttgat gcgatgcggc tcgacggcgg cgtcgcccag gcggcgcttc
     9481 ttgtcctcct cgtagtcggc gaagttcccc tcgaaccact cgacgtggct gtcgccctcg
     9541 aaggcgagga tgtgggtcgc aaggcgatcg aggaagaagc gatcgtggct gatgaccacg
     9601 gcgcagcccg ggaagtcctc gagcgcgctt tcgagcgccg acaaggtttc cgtgtcgagg
     9661 tcgttggtcg gctcgtcgag gaggagcaga ttgccgccgg tcttcagcat cttggcgagg
     9721 tgaacgcggt tgcgctcgcc gcccgaaagc atgccgacct tcttctgctg gtccggcccc
     9781 ttgaagttga accagccgca ataggcgcgc gagttgacct cgatcttctc gccgagcttc
     9841 accatgtcga ggccgcccga gatctcctcc cagacggtct tcttgtcgtc gagatggtcg
     9901 cggctctggt cgacatagcc gatctggaca gagtcaccca ctcggagcgt gcccgcatcg
     9961 gccttctcct ggccggtcag cagcttgaag agcgtcgtct tgccggcgcc gttcggtccg
    10021 atcacgccga cgatgccgcc cggcggcagc ttgaacgaga gcccctcgaa cagcagccgg
    10081 tcgccgaagc ccttggcgag gccgttcgcc tcgaccacga cgccgccgag acgcgggcct
    10141 ggcgcgatct tgatctgcgc cttgccgacg tcctcgcgtc ccgcctcctc ggcgagcttg
    10201 tcgtaggcct gaatacgcgc cttggatttc gcctggcggg ccttcggcga ggatcggatc
    10261 cactcgagct cgcgcttcag ggtgcgctgc ttggcctcct cctggccctt ctcgacctcg
    10321 aggcgcttcg ccttctgctc gagccagctc gaatagttgc ccttgtaggg gatgccctgg
    10381 ccgcgatcga gctccagcgt ccactcggtg atcgtgtcga ggaagtagcg gtcgtgcgtc
    10441 accagcatga tgcagcccgg atactccttc aggtagcgct cgagccaggc gacgctttcg
    10501 gcatcgagat ggtttgtcgg ctcgtcgagg aggagaaggt cgggcttggc gaggagaagc
    10561 cgcgtcagcg ccacacggcg cttctcgccg ccggaaagat ttccgaccgc gctctcgccc
    10621 ggcgggcatc gcagcgcatc gagcgcctgc tccacctgcg cgtcgaggtc ccagaggttc
    10681 tgcgcgtcga tcttgtcctg cagctcggcc atctcggctg ctgtttcgtc cgaatagttg
    10741 gcggcaatct cgttatagcg gtcgaccagc gccttcttct cggcgacgcc ctccatggca
    10801 ttctcgagca cggtcttgtc aggatcaagg tgcggctcct gtggcaggta gccgcccttc
    10861 actccctcct gcgcccaggc ctcgccgacg aaatcgtcga gttcgcccga catgatcctg
    10921 agaagcgtcg acttgcccgc gccgttgagg ccgacgacgc cgatcttggc gcccggaaag
    10981 aacgacagcg agatgtcctt gaggacggtc ttgccgccgg gataggtctt cgagaccttg
    11041 tgcatgtgat agacgaactg ggggttcgcc atgggcggac tcctttgagg gacgcgcatc
    11101 agggtgttgg cgcgcttgta gaggatgggg ggcgagcgct caagggcagg cgcggatcgg
    11161 cgagggagca tgcgaatgga acagacaatc cacattcacg aggatgattg gggtctgcga
    11221 aacctctatc cgctggccgc attcgatgcg gcagtcgcgg atgtcgccgc agccgagaag
    11281 gccggccgcg acaacgtcgc acctgacggc atcggttgga ccagcgtgca cctgatccaa
    11341 gctccgaaag tcgatttcac ggcgacgggc cttgccgtcg atgcggtggc gccgatcctc
    11401 gagcgactgt tgccctgggt tcgccgcttc gtcgcgacgt cgatggcggg cttctcgggc
    11461 ggggcgcgtg acccgtatgg aagctacgag gacgacgcct ggtgcttcgg cttcggctcg
    11521 cactgtttcg tgaagctcga gccggccggc gttctcgttc gtcaagtctg gttcgaggct
    11581 cggacgtcgg acccgtacga actcggcgcg ttacgcaccg ccatcgaggc gatcgatggt
    11641 ctggccgggg cgatgatcgc cgactatgtg ctcgaagcgg tggggcgcgt acgcgaccgg
    11701 acctttatgg agcgctattt atcaagcctc gccgacggcg cctgatggca accgaactca
    11761 ctgcaacctt cactcggccg cgaccgaagg cgcacctgtc tgtcgccctg atcgaggagc
    11821 tttcgtcacg cggagatcga tgtccgtgag cgcccgaccc gttgccgcgc gttccacgtc
    11881 cgggagcaca tcgaggctcg gccagaggcc cgagacgagt gcgcgcgcat atgggtcggc
    11941 aaagcctgct gcaagaagcg agcgggaggc agcggcgtgg tcgtaagtga ggcggtgcgt
    12001 cgtgaggtgc acgccgtcgc gcgtcgacga gacaagaccg taccacgcat ccggcgtgcc
    12061 gtcgttcgcc ggcatgccga caacgccggg gttgaaccag gtgcagccat ggccttccgc
    12121 gacgaacggg acgccgcagt ggccggcaat agtgatgtcg ctcgcggaga gcgccgtctc
    12181 ctctgcgaga acgtcggtca tggaggcgaa cacccagcgg ctgatgccgg cattgccgcc
    12241 gtggacgacc ttgaacgaca agcctgcgta ggagaagcgg agcgctgtcg gcagggcgcg
    12301 catccaatcg cggtccgcgc ggccgatgcg ctgcgaggca tactcgaacc agctcttggc
    12361 gagccggttg caggccgaac cttcgtcgaa attgcagccg caatcaccga ggtcgttagc
    12421 gaggctctcc tcgcagttgc ccgcgacgac gtggatgccc cagtcgcgga tctcgcggac
    12481 ggtctccgcc ggctcggcac agtaggcgac gacgtcgccc gtgcagatcg tgcgctcgct
    12541 tggaatgccg aggtccagcg cacggcgccg cattgcccgg gtggcacgca cattcgagta
    12601 cggaccgccg aaaacgagga tcggcgcgtc atcgctgaag tcgatcacct gtgtctccgt
    12661 actcggcgtg agcgatgacg accctagccg ctcgcgggcg gtggagcaaa tcaccgcgcc
    12721 ttcacgatgc cgtcatggcg gatgatgggc acatgcgtag cggcactctg cgccaggaga
    12781 cctgtcaggc gcgggtcatc cgcgatctcg atctggcggc ggacggcttt gaatccagag
    12841 cgcaggtaga acgcgagtgc gccagggtga tcgagcgtgc aggtgtggac ggtcaagcgc
    12901 gcgatcggcc gcgaccaggc cgtcgccagc gcgaagttca tgaggtgccg ccccgcaccg
    12961 gtgccgagaa gccgcccggt cacgccgaag aaggcgatct cacaggcgcc cgtgtcacga
    13021 aaatcgagct cgagcaaccc ttcgtccctg tcgcgctccc tcagagcgta gacttcgacg
    13081 gaagggtttc tgatgatggc ttcaaggccg gcatcatcga gggccagccg cgagaaccag
    13141 agccaatcct cagcgccgac gcgccggaaa aggtcgcgat accagccggc acgtggctga
    13201 tcgatccgcg cgatggacag ctccggacgt gacgcggcct gccgcggacc cggatcggca
    13261 tccatcgcga ggtgcgtgac gactgccgcg atcttgcccg gcggcacatc agtatagccg
    13321 tcttggagcg acggagatgg cacctcaacc acgcttggcc tcgctgctca aggttgcaat
    13381 ctcggccgcg atgttcgccc gtgcccgcac ttcccagtct gacggcacgc tgagcgaccg
    13441 gccgatggtc gcgaagacgg gggtcactcc ggcgagatgt cggagcgcgc cggcgcggcc
    13501 gttcgcatag ctcgcatcgt cgaactggtg atattcgcgc cggatatcat cggcatagcg
    13561 ccggtagcgc tcggcgggcg cgcccaggat tgcgaggtcg agatcggcaa agtaggcctc
    13621 gtccggcagc aggtctcggg gaccgcctcc caggtgcttc gtcgccatca catagcggca
    13681 ggccttggcg atgacagcat cgtcgacagc ctgcgcccca cactgcgtgc ggagccaatc
    13741 ggcgctcttc tcctcgttgt ccttggccgt gacgtcatag atggcgtcgt gaaagtagat
    13801 ggcgagccgg aatgtggcgt cgtcgatgcc atgagccgcc gccggcttcg gcgtaagacg
    13861 ccggtagtgg tcgatgaact cggcgacgtg ctcgagcgtg tggtagtggc gggaatagga
    13921 ctgatactcg gcaacgagcc ggctcaggat gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnncgcggat acaatcccgc cggcaggaac agccgcggcg
    14101 ccgggcggcc tcacgatcag cgcatcggcc tcggccagcg gcgagaggag cgagctgtcc
    14161 tgcgagcgga cgggcgcgac ctctgccgcg ccatcgccgc cacgccggac aaccgcgcgc
    14221 atgtagtgct gacgtggccc gttggcctcg agcgcgacgg ctgcgcgcac ggccgtcacg
    14281 ccgtcgccgc cgttcggcat cccctgcaag gcgcgaatga gggggacaag gaacacgcgc
    14341 gagcagatca gggccgagac ggggttgcca ggcaacccca gcatgcggat gtcgccgagc
    14401 cggccgaaca tcagcggctt tcccggccgc atcgcgatct tccagaagtc gagctttagg
    14461 ccacggcgct cgagcacggg tgcgacgagg tcgtgatcgc cgacggaggc gccgccgatg
    14521 gtgacgagga tgtcggcatc tgaggcctcg gcgacgtgat gctcgatgct cgtcgcgttg
    14581 tccttggcga tgccgaggaa gcgcacctcc gcgcccgcct gctcggcaag tccggcgaca
    14641 cccagatgat tggaggcgac gatctggccg ggtccgggcg tctcgccggg cagcaccaat
    14701 tcgtcgccgg tcgagaggac ggcgacgcgg gggcggcgaa acacgggcac gctgccgtga
    14761 cccatcgcag cgatgagtgc gagctcacga ggaccgagcc gtcggcctgc cgagacgagg
    14821 ggcgagccgg cggcgaaatc atagccgcgg cgacggatgt tggccgcctc gggcacgccc
    14881 tcggtcaccg tgacggtgct gccggcgatg gtcgcgtttt cctggatgac gatcgcgtcg
    14941 gcgccgtcgg gaacgggcgc gcctgtgaag atgcggacgg cctcgccagg cccgacgtgg
    15001 ccgccgaaag ggtgaccggc ggctgcctcc ccgaggatcg cgagccgcac cggcagtttg
    15061 cgaacgtcac tggcgcggac ggcgaagcca tccatggccg aggcatcgaa tggcggttga
    15121 gtgagacgcg cggcaagcgt ctcggcgagc acccggccgc gcgcctgttc gagcctgacg
    15181 acgtcgcgcg tcaacggcgc gacgcctccc aggatgcgct cgatcgcggc gtctacggcg
    15241 aggagtgcca tggcgtcaga caccctttgc gtcgtcggcg cgccagtggc cggagcggcc
    15301 gccgaccttc tcgacgaggc gcactggccc gatcaccatg ccgcggtcaa ccgccttcag
    15361 catgtcgtag atcgcgaggc aggcgacact tgcggcggtc agcgcctcca tctctacgcc
    15421 cgtctggccg ctgacgccga cctcgaccgt gacggcgatg ccgaccggcg actgcgaggg
    15481 ctcgagcacc acattgacct tcgagaggag cagagggtgg cagagcggga tcaattcgtg
    15541 cgtgcgcttc gccgccatga tgccggcgat ccgggccgtc gccatgacat cgcccttctt
    15601 ggccttgccg ctggtcgcga gcgcgagggt ctccggcgcc atggcgatga acccctcggc
    15661 gatggccgtg cgctgcgtga cggccttctc cgagacgtcg accatgcggg cggcgccgtg
    15721 agccgggacg ccgatggaaa tctggaggcg gctcataccg ggcctctgag cagcatgcgc
    15781 gtcgccgcgg ccacgtcggg ttggcgcatg aggctctcgc cgacgaggag tgtatggatg
    15841 cgggagcggg caagacgggc cacgtcggca ttgttcgcaa tgccgctctc gccgatgaca
    15901 atgcggttcc gaggcaagcg cggggcgaga cgctcgcagg tctcgagcgt cgtctcgaag
    15961 gtcctgagat tgcgattgtt gatgccgacg aggcggcaac caagcttgat ggcgcgctcg
    16021 agctcggcct cgtcgtgcac ctcggcgatg gcgtccatac cgaaatcgcg ggccgccgcg
    16081 gcgaggtcgg cggcgacagc gtcgttcacc tgggcgagaa tgatcaggat gcagtccgcg
    16141 ccccacgagc gggcctcggc gacctggtag gtgtcgagca tgaagtcctt gcgcagcacg
    16201 gggaggccgg acgcctcgcg cgccgcgatc aggtactcgg gcgcaccctg gaacgagggc
    16261 cggtccgtca gtacggagag gcaggtggcg ccgccctcgg cataggcacg cgcgagcgcc
    16321 gggggatcga aatccgcgcg gatgagccct ttcgacggcg aggccttctt aatctcggcg
    16381 atgagcgccg tctcgccggc gctcagccgc gcctcgatgg cgcccgcgaa agagcggacg
    16441 ggcggggcca gcttggcatg ctcgcgcatc acggccggcg gcgtcgcggc ctttgccgcc
    16501 gagatctcgg cgcgcttgta ctcggtgatc ttgttcagga tgtcactcat gcgcggacat
    16561 tacgtcctcg ggccgcagct gtcatctctc tggccggaaa acggaccgga ttgccgaccg
    16621 agatcgcgtt gcaggccgtt gcctgcgacc gcatgttgcc atacatgccg ggcaagccct
    16681 ggggcgcatc ccgtggctgc agccctgcac ccgatcgacc ctaccatgac ccgctccgcc
    16741 tcgaattcag ttccgccgct ccggccgttc gaggccggga cacgtggtta tttcgccgtc
    16801 ggcgcggagc gcatgtcgaa ggccctgaac ctcggcaaca tgatgcgatc cgcgcacgcg
    16861 ttcggcgccg cgttcacctt cacgatcggc gcacgttacc aggcgctgga agcgcgcgcg
    16921 gatacctcca aaggccagca gcatctgccg cactacgagt ggccggcggt cggcgacatg
    16981 cggctccccg agggctgcga actggtcggc atcgagctca tcgaaggcgc cgtcgacctc
    17041 cccgagttcc gccatccgag ccgggccgcc tatgtgctcg gacccgagca gggatcgctc
    17101 tcacccgggc tcatcgcgcg ctgcgaccat gtcgtgaaga tcccgacgcg cttctgcatc
    17161 aacgttgcca tggccggggc gatcgtcatg tacgacnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn tcagcacgga ggcgatcatg gcgctcgagg cactcctcaa gaagttcacc
    17341 gcggccgtcg aggccggaaa cggcaacgcg ctcgccgact gcttcacggc ggacggcgtc
    17401 tacgacgact acttcttcgg cccctccaag cccgggcgcg ccggcatcgt cgagatgctc
    17461 gcccacttct acgagggcgg gaaggatttc cgctgggact tcttcgatat cgctgagggc
    17521 aacccgttcg cctacgcgag ttaccgcttc agctacacct cgacgctccc tgatgcgaaa
    17581 ggtgctcgag tcgctttcgg cggcatctca cggcttcaga tgaagggcgg cctcatcgcg
    17641 cactactccg aggtgttcga tcgcggcatg gcgctcgccc agctcgactt cgcacccgag
    17701 cgactgaaaa agatagcact caagtatgcg gccggcgtga aggcgcggcc tgagtacgcg
    17761 cggcacgcca aggcgtcgtg aactgccgcg gggctgccat acccgagtga atattggcct
    17821 gcccgtcgcc gcgcgatact gctgccgcga gccaacactg cctcgaagga gcccgcccat
    17881 gaccacgagc gccgcgccgc cgcccccgca tccgaccgtg aagcccctgc agggcatccg
    17941 catcatcgac gtgtcgagct tcctcgcggg gcccttctgc tcgacgcagc tcgccgagtt
    18001 cggcgccgag gtctggaagc tcgagcttcc gggcgtcggc gatgccctcc ggaagttcgg
    18061 cacgatgacg ccgtcgggtg actcgctccc ctggatgcag gagacgcgca acaagaaggt
    18121 cgcgacgctc gacttgagga agccagaggg cgccgaactc ctgaagcgca tgatccgcaa
    18181 ggccgacgtg ctcgtcgaga acttccagcc gggtacgctc gagaagtggg gcctcggctg
    18241 ggacacgctg aaggaggaaa acggcaagct catcatggtg cgcatctcgg gattcgggca
    18301 gacgggcccc tactcgcccc gtcccggctt cgggcgcatc ggcaatgcct tcggcggcct
    18361 ctcctacctt gcgggcttcc cggagcttcc gccaacgacg cccggctcgg cgacgatccc
    18421 ggactacctt gccgggctct atggggcgct tggcgtgatg ttcgcgctgc gcgcccgcga
    18481 catgacgggc cgcggccagt acatcgacat cggcctctac nnnnnnnnnn gagccggtct
    18541 tccgcatcct cgacgagctc gccgccgcct accagtacaa gggctaccag cgtgagcgca
    18601 tggggccggg tacggtcaac gtctgcccgc acagccacta tccgacgaag gacggaaagt
    18661 ggatcgccat cgcctgcaca tccgacaaga tctactcccg tctcgccgaa gcccagggcc
    18721 aaccggagct cgcagccccc ggtggccgct gggagaagat cgtcgaccgc gaccgcgagc
    18781 gcaaagacgt cgacgcctgg gtcgagacat ggacgaaaaa gttcacgctc gcggactgca
    18841 tcaaggagtg cgacaagttc caggtgccgt gcggcccggt gtattcgatc tcggacatct
    18901 tcaaggatcc gcactacgcg gcgcgcgaga acatcctgcg catgaacgat ccgcgcgtcg
    18961 gcgagatcgc catgccgaac gtggtgccgc gcctctccga tacgccgggc tcagtcgagt
    19021 ggctcggtcc gccgatgaac gcgaacaccg acgagctcta caaggggctg ctcggcatga
    19081 gcgatgccga gatcggtcgc ctcaaggaca agggcgtggt ctagccgggc tttctcacac
    19141 tctcgcggcc ggcgacgatc atctcggcca atgccggcgc ggtgagatcg ccgagctggc
    19201 gatcgacgat cttgctgccc aacttcagga tcacgacacg cgaggcgacc gccacgacgt
    19261 cggccatggc gtgcgagatg aggacgacgg tgcggccttc ctcggtgagc ttgcggacga
    19321 ggccgaggac gacggcggtc tccttgacgc cgagcgccgc cgtcggctcg tcgaggatga
    19381 cgatgcgggc attccagcgc agtgcacggg cgatggcgac ggcctggcgc tggccgcccg
    19441 agagccgctc gaccgggcgg cgcatgtcgt cgatactgat cgcgagccgg gcgaggtagg
    19501 cgcgcgccga agcgcgcatg gcccgcttgt cgaggatcgc gacccccggc aagagagtgc
    19561 gcgtcagctc agcgcccatg aacacgttct cggcgatcga gagccgcggc gccagcgcca
    19621 gatgctggta gatcgtggcg atgccccggg cgagcgcatc ggccggcgag gcgaagcgct
    19681 gaacgacgcc gtcgagaacg agactgccag cggagggcgc ctccgccccc gacatgacct
    19741 tgatcaggct ggacttgccg gcgccgttgt cgccgcagat cgccaccacc tcgcccgcat
    19801 gaatgtcgag atcgattccg gcgagagctt ccacggcgcc atagcgcttg ctgattgctc
    19861 gcagtgaaag cagcgccgtc acgacttctt cggctcgcca ggctcggtcg gcccgccggc
    19921 ctccttccag gccttgaagc cgccgatcac gtgggcgacg ggcttcagcc ccatgtcctg
    19981 tgcggtcttg gctgcgagca gcgagcgcaa gccgccggcg cagaagaaca cgaatttctt
    20041 gtcttcggcg aagatgggct tgtgatacgg gctcgctgga tcgatccaga actcgagcat
    20101 gccgcgtggg caggagaagg cgcccggcac cttgccgtcg cgcgccagct cgcgcggatc
    20161 gcggaggtcc accagcacga catcgtcgcg gccgtgcagc ttcgtggcgt cgtcgggcgg
    20221 gatctcgtcg acgactttcc tcgcctcgtc gagcatcggc tggatgccgc gcgtgatggt
    20281 ctgggacatg gacactcctt gtgatcactt tcggcgactt tagccggaag cgccgacaat
    20341 tggcgatgcc aattcggtgt cgcccatgcc ttgggacggc catggcgctc ggtgtgccat
    20401 ggccgcggcg tgcaggcctc ctcagttccc cggccccgcg tcgaaggcga ccttgtcgat
    20461 gagctcggag gcgcgcttgc ggaacttggt gatgttgacg agcggcagcg catcgggctt
    20521 cagcgtgccc cagcttgcga cactcgccga gacgggcacc ttggccgaga tcggatactc
    20581 gtagttgatc tcggcgtaga tgcgctggcc ttcatcggag gcgaggaact ccatgagctt
    20641 cacggcgttg tcgcggttct tggcatggcg agcgagggcg acgccggaga tgttcacgtg
    20701 gctgccgcga tccgtgacgt tcgggaaaat gatgcgcacg ctctccgccc aggccttctg
    20761 ctcggggttc gcgagcatgg ccgccatgta gtaggtgttg gcgatggcga tgtcgcagat
    20821 gccggccttc acgtcgcgca ccgcctcgcg gtcgccgccg gcgggcttgc ggccgagatt
    20881 ggcgcgcagc cccttgagcc aggtttcggt cgcggactct ccggcatgcg cgatcatggt
    20941 cgcgacgagg gaggtattgt agacgtgctg gccggagcgg atgcagatct ttcccttcca
    21001 cttcggatcg gcgagttcct cgtaggtgat cgtgtcctgc ttcacgcgct ccttcgaggc
    21061 gtatatcacg cgggcgcgca tggtgaggcc gaaccagtga ccctccgcat cgcggtaggc
    21121 cgcgggaact gtgtcgagaa tcgcgctctt gacgggctgg gttaggcccg cgtccttcgc
    21181 ctcggcgagg cggccgacgt cgaccgtgaa caggaggtcc gccgggctca gcgcgccctc
    21241 cgcggccatc ttttcgttga ggccggcgct ggcatagatg attttgggtc tgatgcccgt
    21301 ctttgccgtg aaggccttga gcagcggctc gatgagcttc ggctcgcggt aggaatagat
    21361 gttgacctcg ccttgggccg cggcagggcc ggagcatagc gaggctgcga tggccgttgc
    21421 ggccatcacc cggcagatgg gcagtgtgct cttggtcatg gtgtcctcct tgctcgaggt
    21481 tcaaggcctc gaaaagagcg cctccttgtt tcaaaggcgg cgagcaaggt caacgaaaaa
    21541 taatgcgaca aatcagtagt ttaaacctat tctaattgta attctggaca gcgcgtccgc
    21601 ggcggtgagt agagcatact cccggcagct cgtggatcag ggcccctgct ggcagagcac
    21661 tccgccggca ggggccgatc cctgtgaact cttgagcggg ccgcattgtc gtcactgccc
    21721 ttcgcaacgt cacctgaccc ttctacacga gtgcgctgag ggtcacgtta actgccaagg
    21781 tcaacgagat gttaaatgac gtaccgtggc ggcgatgggg ccggacgctt cacaagctgt
    21841 cttcgagtcg ccatagatgc tgccatgacg gccggagcca ctggcgcgcc tggagaccaa
    21901 cgtgacgcaa tcgaggaaaa ttcgtgtcgg cacccgcggc tcgccgctgg cactcgcgca
    21961 ggcctgccag acccgatcgc gcctcatgca ggcgcacggc cttcccgaag aggccttcga
    22021 gatcgttgtc atcaagacat cgggcgaccg catccaggac cgaccgctcg ccgacctcgg
    22081 cggcaagggg ctcttcacga aggagatcga ggaggcgctg atcgcgggcg agatcgacgt
    22141 cgccgtgcac tccatgaagg atgtgccgac ggaattgccc gcgggcctcg gcatcgttgc
    22201 ctacctgccg cgcgccgacg tccgcgacgc gctgatcacg gcgacggcgt cgagaatccc
    22261 tgagctgccg gcaggcgccg tggtcgggac atcctccctc cggcgacagg cgcagctgcg
    22321 caatctccgg cctgatctcc gaatcgtgcc gtttcgcggc aacgtcgaga cgcggctgcg
    22381 aaagctccgc gaaggcgaag ccatggcgac gatcctggcg atggccggcc tgaagcggct
    22441 cggtctcgac cagcatgcgc gagccgccat cgaacccgag gagatgctgc ccgcggtcgc
    22501 gcagggggcg atcggcatcg aggcgcggct cgacgatgcc ggaatgtgcg ctcgtcttgc
    22561 cgccatccat catcagccga cgggtatcga gcttgctgcc gagcgcgcgt ttctcgctcg
    22621 gctcgacggc tcctgccgga cgccgatcgc ggcgctcgcc cgtctggagg gcagtcgcgt
    22681 gacgttcaga ggcgagattc tggccgtcga cggcggcaag gtggtgcgcg cgtcgaggga
    22741 cgggagcgcg agcgatgccg agcgcctcgg ccgcgacgct gccgaggagg tactgcggac
    22801 tgccgggccc gggttcctct cggcgggaac ctgaacatcc atgcgcattc tcgtcacgcg
    22861 cgctgccgag gatgccgccg ctctcgccac acgtctcgag gcgctcggcc acacgcccgt
    22921 gatcgcgccg ctgctcgagg tagcctacct gccggaggcc ttggatctcg gtaatgcgaa
    22981 aggcctgatc gtcacgagcc gctatgcgct cgaagctctc gcgcgcgagc cggcagcgct
    23041 tgcggcggca tgccggcgga caatctattg cgtcggcgaa tcgacggggc agcgcgcgcg
    23101 agagctcggc gcgccggacg tggtggccgg gccgggtacg gcgagccggc tcatcggcat
    23161 gatccggtca cagggagtga aggccgacgg gccactcctc tatctttcgg ccaaagaggt
    23221 tgccgtcgat attgccggcg agctgcggct cgccggtctc gatgtgcggc gacagacggt
    23281 ctatcgcatg gttgcagtgc gcaccttcaa gccggcggtc gcccgcgacc tggcggacgg
    23341 ccggatcgaa gccgtcatcc tgatgtcgca gcgcacggcc cgcatctatg tcgaactgat
    23401 gtccagtgcc gggctcgggg tcgcggcgcg gcgcgtccac catctgtgct tatcgccgag
    23461 cgttgccgcc gcactcgcac cgctgggcgg caccttggtc gctgtcgcgg cccatccgac
    23521 gctcgatgat ctgttaacgc tcatcccggg ttagccgatc gtcgtggacc ccctggccat
    23581 gggctcggct tcctggcaca aggacgcagc atcaggtcac agcgtccgtt gcgcttcagg
    23641 tgtgtgcgtt cccccgcgtg ccccaagcca aatgtcgggc gcaagcgcag gagggcagtc
    23701 ccatggcgga caagaaggat cagaagcctc aggcggcgga cagttcaggt cagaagcgcc
    23761 cgtttgcgac gatcgacatc aaggcgacgg aggtcaaggc cgaaggtaag cccgtgccgc
    23821 ccccggccac acccgtgagc ggcggcaagc ccggtgatcc ggcccgtccc gcggacgcga
    23881 aacccggtgc taccggcact cccaagccgt cggatgcgaa gcccggtgat gctgcgaccg
    23941 cgggacccgc aatcgcacca aatgttgccg cagcgacacc accacccgcc cgcggcggct
    24001 ttggtcgtct cgtttcgcac acgctcgccg gcatcgtcgg cggcgtgctc gcgctcggcg
    24061 gcgcgtcgca ggtcgcgccg agcctcggct atccgctgtt cgcgccggct ggccccgtga
    24121 cgcctcagat accagacgaa gtgaagagtc ggcttgccgc catcgaggcg gcggtcgccg
    24181 gcaaggcggc ggcaagcgcc gtacccgcag acgttgcgaa acggctcgcg gtcggcgatg
    24241 cagcggcaca gcgcgccgag gagctggcga aggcgctcac agcgctcaac gcgacgcacg
    24301 ccagactcgc ggccgatacg aaggccctgc aggaggccgt cgccaagcag gcgactgggg
    24361 ccgagctcgc ggccaagctc gccaagctcg aggagacgat cgcctcgatc tcggccgccg
    24421 ccgctgccga cccgcagaag gcggggcgaa tccccgagct cgtgcgcatc gccggaaagc
    24481 tctccgatct cgaggaggcg ctctcgacgc ggctgagcgc gctgcgtaag gacatctccc
    24541 tcgagatcgc gacgcgcacc cagtcggtcg gcgaagccag cgaggcagcc aaggccggcg
    24601 ccacccgcct cgacaaagag gtgacggcga tccgctccga cacggtccgc gtcggccaga
    24661 gggcggacga tctcaaggtc ggcgtcgagc gcctcgcgac gctgctcaaa gggctgcagg
    24721 acgatgccgc gacgttgcgc gccacggtcg atacgctgaa gagcgatctc gatacgcgac
    24781 tgaaggcggt ggcgaagccc gccgacatcg ccgcggcgat cgcgccagtc gcggccaagg
    24841 tcgcctcgct cgatacgaac gttgcaggtg tcgtgcgggg cgaggaggat cggcgcacga
    24901 acgccgagcg catcgtgctc tcgctcgagc ttgccaacct ccgccgcgcg atggatcggg
    24961 gcaagggcta caaggccgag ctcgccgagg tgcggcgcgt cgccggtacg cggctcgatc
    25021 tctccgtgct cgagcgcttc caggacaagg gcgtgcccgc actcgccgat ctcggccgcg
    25081 acttcaacgc gctgatcggg ccgatgctcg acatcgacgc cgagcccaag ggcgcaggtg
    25141 tcgtcgatcg cctgctggcc ggagcgaagt cggtcgtccg tgtccggcgg acatcggcgc
    25201 cgcccgagga caagtcggcg gagggcatca tcgcccgcat ggaaacggcg ctgcgcgaga
    25261 accgcgttgc cgacgtgctc gccgaggcca agggcctaga ggcgcgggtc gcggctccgg
    25321 cgcaggactg gctgaagaag gtggaagcgc gccatgccgt cgacggtgcg ctcgcggcga
    25381 tcgacaacgc gctgaaggcc gcgctcggcg ccaagactgc ggcccccgcg gctgcgacgc
    25441 ccgctgacaa aggggccaag caatgatccg cgtcatcctc ttcctgatcc tgtgcggagt
    25501 gatcgcgagc ggcctcgtct ggctcgccga gcggccgggc acgctcgcca tacgctggca
    25561 ggaccgcgcg ctcgacatca gcttgttccg cgcggtcgtg attctatcgc tgctcatcgc
    25621 cttcgccgtc atggcgtggg cgctgctccg ctatgtctgg cagagcccgg cggctgtcgg
    25681 gcagttcttc atgaagcgcc gccagcagaa ggggctcgac gccctgtcga gcggcatgat
    25741 cgccgtcggc gcgggcgacc ggcaattggc gctgcggtac gcgcagcagg cgcggaaatc
    25801 gctgccgaac gagccgctga cccatctgct ccgcgcgcaa gccgctcagc tgtcgggcga
    25861 ccgcggcacc tcgcggcgga tcttcgaggc gatgctttcg tcacccgata ccgagcagct
    25921 cggcctgcgc ggcctctacc tcgaagccga gaaggaaggc gagcgggagg ccgcgcggca
    25981 gttcgccgag cgggcgatgg tgctcaatcc gcgcctgccc tggccgaccg aggccctctt
    26041 cgagctgcaa tgtcgagaca gcgattggga gggcgcgctt tcgacgctgt cgagcgcgcg
    26101 gaccaacacg ctaatcgaca agccgacggc ggatcgccgc cgggcggtgc tgctgacagc
    26161 gcaggcccag gcgatcgagg aggatgagag cgagaaatcc ctgtcgctcg ccctcgaggc
    26221 gcatcggctc gcacctgatc tcgtgccggc cgcggcaatc agcgcccggg tgctggcctc
    26281 gcgcggcaat ccggcgcgcg tcgccaagat cgtcgagacg acgtggcagc gcgcgcccca
    26341 ccccgaactc gcgacggcct acgcctattc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnngtccgc ctcgccccgc attcgatcga ggcgccgatc gcgctcgcga
    26461 ctgcggccct cgaggccaag agcttcgacg aggcgcgccg ggtgctggag gcgctctcgg
    26521 gcgagcgcca gacacagcgc gtctgctcgc tgatggcgcg catcgaggcg gaggacggtg
    26581 agaatcgcgg caaggcgcgc gaatggctcg gccgcgcggc caatgcgcct cgtgatcccg
    26641 cctggaccgc ggacggcgtc gtctcggacc gctgggcgcc gatctcgccg gcaacgggcc
    26701 gcctcgatgc cttcgagtgg cgagtgccgt cgacatcggn nnnnnnnnnn nnnnnnnnnn
    26761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnn nnnnnnnnnn nnnnnncatg ccggcgcgag tggtcatgcc ggcgggaacc
    27001 ggtgctgcgc gcagcgcgcc ggaggcatcc ggcgagggca agaaagtgat cgagatccgc
    27061 ctcgacggag acgatccgaa ggtcgatttt ccggagcgcg attcgagcga tcccggcagc
    27121 gcacgcgaca aaacaggcgc gcggacatcc ggctcgggca aggcctgatc ggcccgcgag
    27181 caactcgtta cccgtcgctc tcggcgtatc ggcagggcgc atgccgccga tgtcgccgac
    27241 tgcggcgtga cccgctaccg tttggagaca gcaatgcacc tcgacaagct caagcctgcg
    27301 agcggggccg atgtccgcgg tcgccgcgtc atcgtccgcg ccgacctcaa cgtgcccgtg
    27361 cgcgacggcc gggtgacgga cgcgacgcgg ctcgagcgcc tccttccggg catccgcgac
    27421 ctgtcgaaac gcggagcgcg ggtgatcgtg atctcgcact tcgggcgccc gaagagcggt
    27481 cctgaccccg aactctcctt gaggccgatc gcggcggcga tgcaggcggc actcggccgg
    27541 cccgttgcat tcggagccga ctgcatcggc gccgcggcgg gctcagttgc cgccgggatg
    27601 aaggacggag acgtcgcact tctcgagaac cttcgcttcc acaggggcga ggaaaagaac
    27661 gatcccgcat tcgcggccga gctagcgaag ctcggcgagg tgttcgtcgg cgatgccttc
    27721 tcctgtgcgc accgcgccca cgcctcgacc gacgcggtgg cgcggctgct gccgagctat
    27781 gccggaccgc tgctcatgga ggagatcacc gcgctgcgtc gcgccctcga gaagccggag
    27841 cggccgacgg cggccgtcgt cggcggcgcc aaggtctcga ccaagatccc cgtgctcaag
    27901 aacctcgtcg ccaaggtcga caagctgatc attggaggcg gcatggcaaa cacgtttctc
    27961 caggcgcagg gtatcgaggt cggcaagtcg ctctccgagc ccgagttcca tgagacggcg
    28021 cgcgagatca cggcggcggc caaggcgcgc ggcgccgaga tcgtgctccc cgtcgatggc
    28081 gtcgtcgcgc gcgagttcaa ggcggatgcg gcctcgagcg tcgtcctcgt cacggcgacg
    28141 cctgccgacg ccatgatcct cgatgtcggg ccgaagtcgg tggcccacct gatgagcgtg
    28201 atcgccgcct gccggacgat cctctggaac ggaccgctcg gcgccttcga gatcgcgccc
    28261 ttcggcgagg ggacgttcgc gcttgcccgc gaggcggcgc ggctcacgaa ggcaggaaag
    28321 ctcgtctcgg tcgcgggcgg cggcgatacg gtcgcggcac tcaacgcggc gggcgtcacg
    28381 gatgagttca cgtatgtttc gacggcgggc ggtgccttcc tcgagtggct cgaagggcgc
    28441 gatctccccg gcatcgtggc gcttacacga tgacgcgcac gataccgatc gccatcgacg
    28501 ggccgctgcc ggcggccgtc atcttcgatc tcgacggcac gctggtcgac agcgccgccg
    28561 acatcgccga cgcgctcaat acggggcttg ccgtgatcgg cgtgccgccc tttccggtcg
    28621 aggccgtgca cggcatgatc ggcggcggcg ggcgggttgc gatcgcccgc gcgctcgatg
    28681 cggccggccg gacggcaagc gaggacgagc tggcgcgaat cttctcaggc ttcatggcgc
    28741 gctatcgtgt cgtctccagc gagggacgcg gcctctattc cggggctcac gaggtgcttg
    28801 gcggactgag aacgcgcggc gtgaagctcg cgctctgcac caacaaggcc gacgacgtgg
    28861 ctcgcatagc attgcgcgcg ctcggcatcg ccgactattt ccacgccgcc atcggcgcga
    28921 tggacggcaa accgagaaag cccgagccgg ggcagatcca ctggctgctc gatgagctcg
    28981 gcgtcgccgc ccgcgatgcg gtgctcgtcg gagactcggc ggccgatgcc ggcgccgcac
    29041 gatcagcagg gctcggcggg cttattctca cgtcgttcgg ctacagccgg gtgccggtcg
    29101 cggagctggc agcggacgcc gtgatcgatc acctggcgga gctgccgcgc gcacttgcgc
    29161 gatgggccgc gcctcgcaat gtgcgaggtt gaggccaaga gggggcaaaa tgtcgagcat
    29221 gaaagctgcc aagggcccga ccgagacgct cggcgatttc gtcgcaaacc tcacgttcga
    29281 gcagctgccg gggccagtcg tcgcacaagc ctgcaacgtg atcgccgacg ccgtcggctg
    29341 cgccatcggc gcggcggcgg acgatccgga gaaggcgcgc atcgtccgca cgctcgtcac
    29401 atcgttcgcg tcgcggcccg aagccgtgat ctggggcagt gacgtcgcgg cagacccggc
    29461 gctggcggca ttcggcaacg gcattctcgt caacgcgacc gacttcgacg acacgcacaa
    29521 gcgggcgctg ctgcacaccg gttcggtcgt ggtgccgccg gcactcgcac tagggcaggc
    29581 gctgcatctc gatggccggc gcatcgttac ggcggtcgtc gccggctacg agacggcggt
    29641 ccgcgtcggc atggccgtga tgccgacgca ctatcgctac tggcattcga ccgccaccaa
    29701 cggcacgttc ggtgccgcag ccgcggcggc gtccgccatg agcctcgacc cggcgggtgc
    29761 ccggaccgcg ctcggacatg ccggcacgca ggccgcgggc ctcaacacgt tctttacctc
    29821 gggcgacttc accaagagcc tgcatccggg caaggcggcc ttcaacggcg ttctgtcggc
    29881 ccggctcgcc cgcatcggcg cgacgagccc gccgacggtt ctcgagcacg agaagggcta
    29941 cctctccgcg ttctcggcgg agccgaaggc cgcagctctc acggcgggcc tcgggaaaac
    30001 gtgggaaatc ctccagaacg gcttcaagtt cttcccgtca atcctcgcga gccatagcgc
    30061 cattcaggcg acgctcgatg ccgtccgcga ggcgcgaccc gatccccgcg cgatcgtctc
    30121 gatccgcaac gagacctacg agacggtgcg cacgcacttc tcggcaaagg ctgtcacgac
    30181 cgagatgggc gcgcgggtga gcgtgcctta ctgcgtcgca gttgcggctc tcgatggcac
    30241 cgtgggacag gcccagttca cgaccgagcg cgtcaacaga accgacgtac aggcgctgct
    30301 ggcgcggacg gaaacgattg ctgatcccgc gcttacaacc ctttacccgg cgaaatttcc
    30361 ggcccgtgtg acgatcacgc tcgccgacgg gcgcacgatc gtccgcgagc gggcataccc
    30421 gaagggcgat ccgcaggagc cgctgacgcc tgccgagatc gaggcgaagt tcctcgccaa
    30481 tgccgagcgc ggactggggg ccacggccgc gcgcgagttg ctgagcctga tccgcgcact
    30541 tcccgacgag cgcgaattct cgcgcctcgg caagctcctc tgtccgcgcc cggcaccctc
    30601 gcgcgcatcg cggtagacgc ggcaggccgg gcgacgtagg cataggagat caagaacacg
    30661 ttcccctggt cctgcgagga gatgctccca tgaagctcgt tcgtttcggc gcgcccggcg
    30721 ccgagaagcc cggtctcgtc gatgccggcg gcgtgatccg cgatctctcg cagcacgtga
    30781 aggacatcac cggcgacacg ctttcgcagg ctgccctcga caagctgcgc aagctcgatc
    30841 cgaagtcgct gcctgaggca ccgaagggcg tgcgcctcgg cgcaccggtc ggtggtgtgc
    30901 ggaacttcgt ggcgatcggc ctcaactata cagaccacgc caaggaggtc ggcgcgccga
    30961 tcccagccga gccgatcatc ttctacaagc tcgccaactg catcgtcggc tgcaatgacg
    31021 acgtgatgat cccgcgcgcc tcgacaaagc tcgactggga gctcgagatc gccttcgtag
    31081 tcggcaagcg cgcccgctac gtcgaattga aggatgcggc cgcccacatt gccggctaca
    31141 cgatctgcaa cgacatttcg gagcgcaact tccagctcga gcgcggcggc cagtggatga
    31201 agggcaagag tgccgagacc ttcggcccgc tcggtccctg gctcgtcacc tccgacgagg
    31261 tcaaggacgt gaacaacctg ccgatgtacc tcgacgtcaa tggcaagcgc atgcagaccg
    31321 gctccacggc gacgatgatc ttcaagtgcg acttcatcct gcactacgtc tcgcagttca
    31381 tggtgctcga gccgggtgac gtcgtgacca cgggcacgcc cccgggtgtc ggcgcgggca
    31441 tgaagccgcc gacattcctc aaggctgggg acgtaatgga gctttcgatc gagggtctcg
    31501 gccggcagaa gcagaaggtc gtgcccttca agctctgatg acgcacgagc ggcatggcgc
    31561 gcgcataaca aggacttggc aatgccgctc atcgacatcg atcgcgtctc ggccatcgtg
    31621 cgcgaggtgg gccagcgcgt ggtgctgccg cgctggcgca atctcggcga gcgtgacgtc
    31681 gtcgagaagt caggaccagg ggatctcgtc accgttgccg acaaggcggc cgaggaggcg
    31741 ctgacgccgg ccctgtctgc gcttgttgcg gggtcggtcg tggtcggcga ggaggccgtt
    31801 catgccgatc cgacccggcg cggactcttc gatggcgacg gcccggtctg ggtcatcgat
    31861 ccgatcgacg gcacgagcgc gttcgcgtcc ggaaagcccg agttcttcgt aatggtggcg
    31921 cttgtttccg gccgcgagct cgcagcgggc tggatctacg gcccggcgag cgacacgctc
    31981 tatgctgccg agcggggcgc cggcgcgttc agggtgcacg gcgacgggcc acacgagcgc
    32041 attgtacgcg tggtgccgcc agctgagtta tcggcctttc gcggcgctat cggccgacgc
    32101 tatctctcgg ccgaggagca ggcggcgatg gccgcgcgat tgccgcgtgt cgggccttgc
    32161 gaaacggcac tctacgccgg ccgcgaatat ccgcgactcg tctcgggcga gctcgatttc
    32221 ctgctctacc ggcggaccga gccctgggac cacctgcccg gtctcgcgat cgcgtccgag
    32281 cagggcttcg cctattgccg ccacgacggc agcgcgtacc tgcccggcat gaactcgggc
    32341 ggcctcgtga tcgcacccga gctctggctc gagcgcgtca gagcgaactt cttcgcctga
    32401 ggccgacttc gcacgacgag ctcgggcatt ggtcccttgc ggaacaacca agtcattggc
    32461 ggatcgtttc gtccaatcac ggcgattgag cggtgccttt caacagcgac agacgtccca
    32521 acagcagctc ggaaacgaca acggcatcac cacgggtaag agtggcatcg cctacagcca
    32581 tgagggtgca caagtgctcg ataggcgagg gcggcacggg gttgggagcg gtcggctttc
    32641 gcatggcgcg agtattggac gaggggcgga gccgtgcaac gctgatgatc ctcgcctaag
    32701 cagcctagtt gggcttgccg ttgagagggg cggctaaggt ctcaccggtc tgctgggcgc
    32761 gcatgcggcg aggaacatcc gcgagatggg catggcgtgc ggttgagcgt ggcgtcatgt
    32821 ggctgagctg gcgttgtcag aaactggcca cctgggctcg tagcaccacg caaagcgggg
    32881 cccggcgatg cgccgggccc cttgtggccg accggggaga tgggaggaga ggcggccggc
    32941 cgaacgtcgt cagggcgcgt tgcggtcgat ccagatgaac accggctgct tcggcgcggt
    33001 ctggttgagc gtgtcgaaga tgtcggcgcg cggcgccgtc tggttcagcg tctcgaacac
    33061 atccgagcgg ggagcgacgc ggtcaagctc ggcgaagatc ggatgcttcg gcgcgctctc
    33121 ggcgagcgtc tcgaagacgc ccttggcgga agcggggccg gagatcgcga cgacggcggc
    33181 ggcggcgatg gcggagatga tcgtcttcat ggcttgggtc ctttcggttt gcagtattgg
    33241 gtctcagctg cactatcggg gattgacgtt tgacgtccgt acgagtgaag gggcatttgc
    33301 gcccctcgga tcacggctcc ggtcagggcg cgttgcggtc gatccagatg aacaccggct
    33361 gcttcggtgc ggtctgattg agcgtgtcga agatgtcggc gcgcggcgct gtctggttca
    33421 gcgtctcgaa cacatccgag cggggagcga cgcggtcgag ctcgacgaag atcggatgct
    33481 tcggcgcgct nnnnnnnnnn gtctcgtcgc gcctgttcac gcttagggga ggcggccttc
    33541 cgcatggctc cgaatgagct aactgtgcgt tcagacgaat tggcgcaaac gccggcttag
    33601 ttcgtccgca acaccaatca aagggaggaa gcacgatgcc catctctgca gggacggatc
    33661 ggaggccgaa cgagagtggg gcattgagat cgctctgctc tcgtctcggc ggggcgctga
    33721 ttgctgtctc ggtcatcgcc ggcacggcaa ccgcggcgcg cgccgagagc attgaggcga
    33781 ccttcgcgcg ctacgccaag ggcagcgccg cagccgtcga ccatggagtg tgggcgcgtc
    33841 tgctgactga gcacgtcaag cctggagccg acgggctcaa tcgcgtcgac tacaaggcat
    33901 tcaaagccaa gggccacacc gacctcaagg cctatatcgg cagcctcgtc gtcgtcgatg
    33961 tcgccaagct cgatcggcca gagcagttcg ccttctggtc gaacctctat aacgcgaaaa
    34021 cgatcgacat cgttctcgac aagtaccccg tgacgtctat caaggacatc agcctcggcg
    34081 gcggtatcct cgccagcatc accggcgggc catggaaggc aaaggtcgtc aaggtcatgg
    34141 gccatgacct ctcgctcgac gacatcgagc atgtgatcct gcgggcgatc ttcaaggacc
    34201 cgcgtgtgca ctacgcggtg aactgcgcct ctgtcggctg cccgaacctg atgacgacgg
    34261 cctttaccgg agcgatgctc gaagcccagc tcgaggccgg tgccaaggcc tatgtcaatc
    34321 atccgcgcgg cattcgcgtc gagggcggtc gcgtctatgc ctccaacatc tacgactggt
    34381 tcaaggccga cttcggcggc tcggccgacg gtgtgctcaa gcacgtgcgt gtctacgccg
    34441 accctgcgct caagcagaag ctcgaaggcc taacccgaat ctacgattac ggctatgact
    34501 ggagcctcaa cgatgttgcg cgctgaccct cgcccggaga cgccgaccat ggcgccacag
    34561 accagtgttc cgacgggctc attccggcgc ctcttgccgg cagtcgcgct gattgccgtg
    34621 atggccgtcg cctactggcg cggttggcac cagtatcttt cgttcaagac gatcggcctc
    34681 aactatgagc tgctcaaggg cttcatcgag agcaaccttc tcggtgcgct cgcgattttt
    34741 gtggcggcct acattgccgt cgtggcgctg tcgcttcccg gagcgctcat catgtcgctt
    34801 tccggcggcc tgctcttcgg ctggaagctc ggcacaccgg cgatcgtact cggcgcgacg
    34861 acgggggcga cgatcctatt cctgatcgcc aacacctctc tcggtgaacc gttggcgagg
    34921 cgcgccggcc ccgcgctcac aaagctccgc gaaggtttcc gggccaacgc gatgagctat
    34981 ctgctcttcc tgaggctcgt cccggcgttc ccatttgtcg tgatcaatct cgccgcagcg
    35041 ctgctcggcg tgccactgcg cacgttcatc ttcggcaccg cgctcgggat catcccgggc
    35101 tcgctcgcct acgcgacggc cggttccggc ctcggctccg tggtcgaggc tcaaaacgcc
    35161 gtccacaagg cctgcctcgc caagacaccg gcaaatccgg acgtcgcctg cccctacagc
    35221 atcgatacct cggcgctgat caccaaggag ctgatcgccg ccttcatcct gctgagcgtc
    35281 gtcgcgctca ttcccgtcgc catcgagaag tggagagccc gccatgcagc gtaaagcgac
    35341 ccctgatgcg ccggccgttc cgccgggcgc gcgctcctac agtgaagaga tcaaggccga
    35401 tctgtgcgtg attggcgccg gctcgggcgg gctctcgatc gctgccgccg catcccagct
    35461 cggcgtgagc gtcgtcctta tcgagaagca caagatgggc ggcgactgcc tgaactacgg
    35521 ctgcgtccca tcgaaggccc tgatcgcggc gtcgcggcgc gcgcacgcca tgcgatcgag
    35581 cgcaccgttc ggcattgctc cggtggtccc ggcgatcgat ccgcgcgcgg tgcatgatca
    35641 cgtgaagggc gtgatcgcgg cgatcgcgcc taatgattcg gtcgagcgtt tcacggggct
    35701 cggcgtccgc gtcatccagg ccgcggcccg cttcctcgac aagtcgacgg tgatcgccgg
    35761 cgatacggcc gtgaaggcgc gccgcttcgt gatcgcgacg gggtcgtcgc cggtcgtgcc
    35821 gccgatcccg ggactcgtca acgtgcccta tttcacaaat gagacggtct tcgacaacca
    35881 gcagaagatc gaccacctga tcgtcatcgg cggcgggccg attggcatgg agctcgcgca
    35941 ggcccacctg cggctaggcg cccgtgtgac cgtgctcgag ggcctcaagg cactcggcaa
    36001 ggacgatccc gagctcacag acgtggttct caagagcctc cgcgccgagg gcctggtcat
    36061 acgcgagggc gcgcgcgtcg agatgctcga gcgcggtgcg gggccggctg ccgtccgcgt
    36121 taccatcgcg acgggtgagg ccaaggagat cgtcgagggc agtcacctgt tgatcgccac
    36181 tggccgcaag ccgaacctcg ccgacctcaa tctcgatgcc gcgggcgtca agttcgacaa
    36241 gcgcggcatc caggtcgaca agggattggt gacgtcaaac tcgaaggtct tcgccatcgg
    36301 cgacgtggtc ggcggcctgc agttcacgca cgtcgccaac taccatgccg gcatcgtctt
    36361 ccgccgggcg ctcttccgcc tgcccgcgaa gacccgtgat gacattgtgc cctgggtcac
    36421 ctacaccgac cccgagctcg cccatgtcgg gctcgccgag gacgcggcgc gcaaggcgca
    36481 tggtaagatc aacgtctacc gctttccctt ccacgacaac gatcgtgccc aggcggagcg
    36541 ggcgacgcac ggattcatca aggtcgtgac aagcgcgaaa ggcaagatac tcggcgcctc
    36601 gatcgtcggt gagcacgccg gtgaactaat ccaaatgtgg tcgctcgcgc tgtcgcaggg
    36661 tatgaatatc aagcagatga cccagtggat ctcgccatat ccgacgctgt cggaggtgag
    36721 caaacgggcg gctctcggtt attatgcggc tcaggcaggc agcccgctcg cccgcaaggt
    36781 cgtcggctgg ttgcgggtct tcggctgaac gggacgcacc cgacgggatg tggccgggtg
    36841 cgccgttggc tattgagctg gccgctggtc taccgggggc ggcgccgcag gtggcggcgc
    36901 ggatcacaat cgtcgggaga gagcgtggac gcacgggtgg agcgaggcgg aagcgaggac
    36961 gcgggggcgg catcggccgt ccgccgcggc gtgctcgcgg gcgtcgtcga tcgcctgcgc
    37021 cgtcctggcc tctcgaccaa gctgctgcgc ctcaccgtgc tcttcgtgat gctggccgag
    37081 gtgctgattt tcgttccctc catcgccaaa ttcaggctcg actggctgaa cgaccgcctg
    37141 aagtcggcgc acctcgcctc actcgcgctc gaggcggttc cgggcggtca ggtgccggca
    37201 acattgcgca aggagctgct cgaaggcgcg cgcgtcaggg ctgtcgccgt ccgccgggcc
    37261 gaccagcgcc tcctggtgct gccggccgat gggccgatca tcgtcgatcg cacctacaac
    37321 ctcgccgaga tgcagcccgc aggcagcaca agccccgtgg tatccggacg gcggatcgag
    37381 gccgcctacg atgcgctgat cacgtacttt ctcgcgagcg agacctacat ccgcatcatc
    37441 ggccactcgg gcatggccgc gtctcccgag cgtgacgtga tcgacgtcgt gctccccgtc
    37501 ggcccgctca agtcggcgat gatccagttc gggctcaaca tcctcgccct ctcgatcgtg
    37561 atctcgatca tcacggccgg cctcatcttt ctggtgctcg accgcctgct cgtcaggccg
    37621 atcatgaaca tcgccgacaa cgtggtgcgc tttgccgcca accccgagga tcagcaggcc
    37681 ttcatcaagc cgagcgggcg caacgacgag atcggcacgg ccgaacacga gatccgccgc
    37741 atgcagcagg agctgagcca ggtcatccac cagaagagcc ggctcgcggc ccttggtctc
    37801 gccatgagca agatcaacca cgatttacgc aacatgctgg ccaatgcgca gctcatctcg
    37861 gatcggctta cgacgctacc cgacccgacc gtccagcagt tcgcgccgaa gctgatcgcc
    37921 tcgctcgacc gggcgatcaa cttcgccaac agcacgctgc attatgggcg cgccgaggag
    37981 gctgtgccca agcgcgagcg gctcgccctc aaggaatttg cccgcgaggt cggcgagggg
    38041 ctcgggctgc cgcgcgcgca ggtcgccttc gaagtcgaga tgccggacga ccttgtcatc
    38101 gatgccgatc ccgatcagct attccggatc ctgaccaaca tcatgcgcaa ctcgatccag
    38161 gcgatcgagg cggaggcgca agtgcgcaat cgcatcgtga tcggcgggcg gcgcgagggg
    38221 ccgatggtca cactgtcaat cgcggatgac gggccgggcg tgccggcaaa ggcgcgcgcg
    38281 cacctcttcc aggcgttcca gggctcgacg cgcaagggcg gcaccgggct cggcctcgcg
    38341 atcgtgatgg agctcgtcgc agcgcacggc ggccgcatct cgctccgcga cgacgtgccg
    38401 aagggggcga cgttcgatat cgagatcccc gatcgggcag ggtgagcgca ctctcacgcc
    38461 ggccgcgcga tctcctcggt catgaacgcg acgaggtcgg ctcggtcgac gtcgcgcgtc
    38521 acgaacgcct cgccgatgcc ccgggccagg atgaaggtga gctggccgcg ctgcaccttc
    38581 ttgtcctggc tcatcagcgc catcagctcg tcgacgctcg ggagactgtt gccctggatg
    38641 tgggcgatct ccgtcggcag gccgacggcc cggagatgct gcatgacgcg ccgtgctgca
    38701 gccatcggag cgatcccgca gcgctccgaa taccggaacg cctcggccat gccgatcgcc
    38761 accgcctcgc cgtggaggag gcggctcgag aaccccgccc aggcctcgag cgcgtggcca
    38821 aacgtgtgcc cgagattgag cagcatgcgg tcgcctgtct cggtctcgtc acgagcgacg
    38881 atcgccgcct tcatggcgac gctcgtctcg atcgcgtgcg tcagggcgtc gcgatcgtgt
    38941 gagaggacac gggcatgatg ggcctcgagc caggcgaaga acgccgcgtc gccgaggagg
    39001 ccgtatttcg ccacctcggc gtagcccgcc ttcagctcgc gctgggggag ggtcgtgagc
    39061 acgtccgtgt cggctaggac gaggctcggc tgatggaagg cgccggcgag gtttttgccc
    39121 tgcggggtgt tgatcccggt cttgccgccg accgagctgt cgacctgggc gagcagcgtg
    39181 gtcgggatct gcacgaaacg tacgccgcgc cgcacgatcg ccgctgcgaa gcccgcgagg
    39241 tcgccgatca cgccgccgcc gagggcgatg acgaggtcgc cgcgctcgac gccgcgctcg
    39301 agcagccatt ccgagagccg ggcgagctcg gtaaagcact tgctgcgctc gcctggcggg
    39361 agagcgagtg cgccgcgcac ggtgacagag cccgcgagcg ccctctcgag gcgagccaga
    39421 tggtgcgcgg ccaccgtctc gtccgtgacg attgcaacgg cggcccgcgg aaagcgcgcg
    39481 gctatctcgc accccgccgc ctcgatcagc ccgccgccga tcagcacctc gtagcttcgc
    39541 gcgccgaggg ctacgtgtac cttgcgcacc ggaccggccg ggctcggaga cgtcacggtt
    39601 caacctccac gtggtccgcc tgccggcgga cgagagctgc catgatctcg tcgacaatca
    39661 tttcgtgcgg cacctcgcgg ctttcgatcg tcacgtcggc ctcggcgtag accggatagc
    39721 gcgtcaccat cagctcggcg agcttgctgg cgagcggccg gttgctgcgc cgctccacgc
    39781 ggcgctgcag gattgccagc tcggccttca gccagatcga cacgccgtgg ctgcggatca
    39841 tggcgcgggt cgcggcgctc atataggcgc ctccgccggt cgagagcacg cgaggcgcgg
    39901 tctgcagcag acgcgcgatg atcatgctct cccgctctcg gaaatacggc tcgccgtgat
    39961 cggcgaagat ctcggggatg gtcttgcccc actgcggatt gacgctcatc gcggcctcga
    40021 tccgaatcga tgaactgcag gccgaggcgc agggcgagcc gccgaccgat ggtcgtcttg
    40081 ccacagccca tgaggccgac caagacgatc gagcgcccgc ccagcccggc aacgatcgca
    40141 ggcgcccgtg gatcatcgat gcgcgcgcgg cgtccaccag tccgaatcac ctcctcggtc
    40201 atgtgccgcc ttgggtcctt ccggcgctgg tcgtggaaac caccaagata tggtatgatt
    40261 cccgtgtcat tttgagttcg cctttgtcgt caggggcctg ccgcagcggg cggatcgttc
    40321 gtcgtaggaa ccacgttgct cacggtttga ccagtggtcg cgccggccat attggcgtca
    40381 aactgatcgt cgattactca tgcaacgcca gggtctcaca tgccaagcct cgtccgcttc
    40441 ttcgtggttg tcgggctgct ctccgcagtg actttcggcg gtctctatat catgtccgag
    40501 tacttcgagc ctgatcagcg ggaggtcact cgtcccgtgc cgggcgtcaa ggtgcgcaag
    40561 tgaggcggcg ttcggcgtgc attggtggcg cctccggccg gaggcaggaa gcatcggggg
    40621 cggatggacg tgaatagcag gctcacccgg tctcgctcgg gccgcgcagc cggtgcactt
    40681 gcggtgattg cgctgctcgc cgccgccgtc ggtctcgccc cgcttgggtc ctctcacgcc
    40741 gaggatgcct ggaacccctt cgccgagaag gacgcggcac gtaagccgag ccgcggtgct
    40801 ccgccgttgc cgtcagaccc gcgacccgct ttgccgccga tgggtggacg ttaccctgat
    40861 cccgagggcg caccgtcgcc cggtgacagt gctccgccgg gctacccgcc ggcaggctat
    40921 ggtccggccc cgcgaggggc atcagctccc agtcccgccg atgccggtcc gccgacatac
    40981 gctgcgcctg cgtcgcccgg ctattcggcc ttgcctccgt ctggcggggc ccacgatggc
    41041 gtgacggtcg cgccgctggc gccgcccgat ggcacgcccg cgtttctcgc cccgtccgga
    41101 cccgcggctt cgaaggtggc tccgacaaca gcaaggggcg ggctgccacc caccctctgg
    41161 gaaggagccg acggtccgac catcgaacgg ctcgtcggcg cgatccccga tccaggccga
    41221 tcggcagcgc tccaggcgct ctggcggcag gtgtggacgc accgcgcgcc ggctcccgag
    41281 ggggccaagc agttcgaggc aatccgattt gaggcgttct ggcgggccgg gctcgtcggc
    41341 gagatcgtcg cgcgggcgaa ggcggtggac gcgtccgatc cgctcgtcgc gctcatgctc
    41401 gcgcgcgcag agatcgcgct tggcgagcgg gagacgggtt gcgcccgtgc ccggcccgtt
    41461 gccgcgcata tgaagtcgct ggtgccggcg cttgccgccg aggcgctggt gatgtcgagt
    41521 tattgcgcga cgtcggccaa ggggcgtggt gcggccgagc ttgccgcggg tctccttcgc
    41581 gaatccaagg tggacgcgcc cattccgctc gccgtgctcg acgcgctgtc gggttcggtc
    41641 cgtgggagcc cgcagctgcc acgcgtcgtg cgagcgatcg actatcgctt cctcgagctc
    41701 ggcggaaccg tcgatgcggc cgccctcgtc gaacgggccg aacctgcgct cctcgccatc
    41761 ctcgccgaac tgccgggcga cccgcgggga cgcgtcctcg ctgccgaggc ggcc
//
LOCUS       DAORNH010000008        40852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_730_length_40852_cov_2.664828, whole genome shotgun sequence.
ACCESSION   DAORNH010000008 DAORNH010000000
VERSION     DAORNH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 40852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40852
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_40852_cov_2.664828"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..>239
                     /locus_tag="PK264_01580"
     CDS             45..>239
                     /locus_tag="PK264_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74620.1"
                     /translation="MAGSRFAVLPRGMPALLLVAFAGADLAVACAPPPPAVTTIALPR
                     FYADKEGSEVDPKLKAAHAAA"
     assembly_gap    240..359
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <360..586
                     /locus_tag="PK264_01585"
     CDS             <360..586
                     /locus_tag="PK264_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74621.1"
                     /translation="LAAWADAGALTGRMETRQAEYQRKFDFVGLALAWVKVRPHASEV
                     ERRAIDAWLIRLADLSRAFFDDRGNRVPAV"
     assembly_gap    598..607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <608..1119
                     /locus_tag="PK264_01590"
     CDS             <608..1119
                     /locus_tag="PK264_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRD74622.1"
                     /translation="RGRKRNNHWYWLGAGLAATATTTDSERHWKLAREIYADAAREIT
                     AEGFLPMELERKSRALYYHAFALMPLVTMAELASARGENWASLGDDALHRLAERTRAG
                     LLDSGPFDKRAGAAQERPLKPGAGWLELYAIRHADRLQSPRVDMPPGHRWLGGDVRLL
                     AEALKPRAN"
     gene            complement(1189..2622)
                     /locus_tag="PK264_01595"
     CDS             complement(1189..2622)
                     /locus_tag="PK264_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74623.1"
                     /translation="MLGEKEVGFGVDRDVIEVGRSEGRFKALKLIVRKSDVFLIDLKV
                     TYGNGQQEDLAVNQLIRAGTETPMLDLKGEARAIQRIDMVYRSRPGYAGRAVVEVWGL
                     QHVRVKDRVPPGYAELDVQTFDVRKDEIRMPVGWKEGAYKAIRLRALDEPLFIRRVQI
                     RYENGYTEDMRVRQAIAPGEMTSEIDLAGERATHIREVIVSLRPQPSARGNATLQLLG
                     DSNRRFAIPPGYGAPPPPPPRPSYGPALSAVPRGWVLFGTQTVGFKAERDTIAVGREV
                     GRFDKIAFRVSGNDILMRDIEIVFGNGETQRFPINAEIRANSMTPALDIGGTRFIREI
                     NMTYQAKPNFKGRATVEVFGEYAASWVSGDAKKHNDGWVLLGSQRAAMLKNDVDTFQV
                     GRQWGTFRAIRVTAKGHAVRVNWLRVTYANGETEELPVNRELRGGETSSPIDLRGRER
                     VIESIAINYRSRLNLGGEAVVEIWGLH"
     assembly_gap    3118..3142
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3143..>3513)
                     /locus_tag="PK264_01600"
     CDS             complement(<3143..>3513)
                     /locus_tag="PK264_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRD74624.1"
                     /translation="DFMKAASEARIPEGVQQIAEDTVAKTREAYARMAVATKDSAKVA
                     EEVFLAAHAGARVIGEKMLANTAANTEAMFDAAQAIVKAKTLPEAARLQATFVQSQFA
                     IAGNQAKELFELSTRIAQQTF"
     assembly_gap    3514..3539
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3764..5059
                     /locus_tag="PK264_01605"
     CDS             3764..5059
                     /locus_tag="PK264_01605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD74625.1"
                     /translation="MHQTAPYWWDAAPPAMLPTSPVRPKCDVVIVGAGYTGLSAAITL
                     ARAGRSVQVFDKMRPGEGASTRNGGIASGNLRPSMKELTKTFGEARALGIQAESKAAR
                     DDLAAFIQREHIDCDFKIAGRFTGASSPAAYDDLARDAEMLTRTLGIEARAVAKSEQH
                     QYLGTDFYHGGLYRNDIGGLHPAKFHAGMLRVAIAAGAVIHGETAVLGMRREGDRHEV
                     ATARGTVSADQVIVGTNGYTDKADTWLRRRLVPVRSRIIATEPLSNNLMRELMPKGVM
                     CSETRKLHYYYRPSPDGTRILFGGRDGTITGDSPRPTESLRRALADIFPVLSGTPITH
                     SWFGYVAMNRDMVPRIFSRSGMQYATGYCGSGVVWARWAGQKAALQVLGNADGQSSLD
                     FRPPGMVPVVGGKAWFMPGVFAWLTLQDKLAARKRKQTG"
     gene            5176..5760
                     /locus_tag="PK264_01610"
     CDS             5176..5760
                     /locus_tag="PK264_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74626.1"
                     /translation="MTDKPDQNDSRIAPDPIAAVLPALAALGTIASIATVNWVAHDKT
                     PDRTRMRRKAQTALRDLESCCMGLQEIFKRLYRNPKLFAGEGGAASSPMKFGVHGPRI
                     DPTLARTFHQLMNDIASMLVLASQNAYDVMCAVEDGEIIAPEELFFGFGEQQERLNKL
                     FTQRATLRLSVETGLDIADRLTALVRELKRFKID"
     gene            5812..6699
                     /locus_tag="PK264_01615"
     CDS             5812..6699
                     /locus_tag="PK264_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD74627.1"
                     /translation="MRFMNVTLDDDDVPQLALVEGNKVRLLGDAADMDVPMSLDELIM
                     MDPRLESFQEILRENPDLPTVPLEKVRADLPLDFIDKVVCLGLNYLEHAKEGGHAPPT
                     YPSFFLRAPSSLVAHGQPVIRPKVSEQLDFECELVIVIGEEGRHIAEKNALKHVFGYT
                     LMNDVSVRDYQRKTTQWTAGKNFDGTGPLGPMVVTADELPPGAKGLRIQTRLNGKVMQ
                     DANTSDMIFDVAKTISLLSEIMTLRPGDLIATGTPSGVGHARKPPVWMKPGDVVEVEV
                     EKIGVLRNPVIAEGDEPDA"
     gene            complement(6963..8384)
                     /locus_tag="PK264_01620"
     CDS             complement(6963..8384)
                     /locus_tag="PK264_01620"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRD74628.1"
                     /translation="MPMDEAQRNFPLEDIDRECVFHPNTSITDHMKKGPVIYSDGRGV
                     RIRDQKGRDIIDCGAGLWCVNIGYGRPEMAEAAKKAVESLAYHHIFGGASNEPAIRLA
                     EKVLKLFHERADAPQMSKVFFGMGGSDANDTNYKLVRLYNALRGKPQKKKFIARLGGY
                     HGLTFAAASLTGIPAYHKAWDLPMGDVVHVSCPHYFRYGNPGETEEQYCDRLVAEIEG
                     VIAREGADTIGAFIAEPVMGTGGVLIPPKGYFEKVQKVLAKHDILFVVDEVITGFGRT
                     GHWFSTGGMKLKPDIVTLAKGITSAYFPVSASVIAAKVWDVLAKASPEYGPVMHGFTY
                     SGHPVGAALGLVNISIMERERLVEQAAEVGPYFLKGLRERIGDHPFVGEVRGIGQMIA
                     AEFVADKGKKRFFDPKAGVHRLVSAKAFEHGVLSRALPFIEVTSFSPPLSMTKAEADE
                     AADRYAKALKEATDDLAKLAKPA"
     gene            complement(8486..9121)
                     /locus_tag="PK264_01625"
     CDS             complement(8486..9121)
                     /locus_tag="PK264_01625"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HRD74629.1"
                     /translation="MTDPSAAPGPDPTTPAPNVIVPPGWERAHDCSVSFAMANLAGLL
                     GILPITALLGWVFSVVWTFDRLVLGAALGPLTFLAVFLVGIIAHELLHGVTWTLLARQ
                     PRRVVRYGFKWTTLSPYANALVPLPARDYRIGIMMPGVVLGLLPAFVAIATGHGALMS
                     VAILMTIAAGGDLLVLWLLRGKAAQSLVLDHPSRVGCLVLDVPSDPSTART"
     gene            complement(9241..9450)
                     /locus_tag="PK264_01630"
     CDS             complement(9241..9450)
                     /locus_tag="PK264_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRD74630.1"
                     /translation="MQGGTVKFYNDQKGYGFIKPDDGGKDVFVHATALERAGMGPLTE
                     GQKVMFDTATDRRSGKIAVANIKAA"
     gene            complement(10257..>10682)
                     /locus_tag="PK264_01635"
     CDS             complement(10257..>10682)
                     /locus_tag="PK264_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRD74631.1"
                     /translation="EPYLARRRACGWGLYSTLGITRDDKDGMKKQRAENYDFFGAPTG
                     LIFTIDKDMELGSWLDTGMFIQSIMIAAREFGLETCPQAALCNFHTILMKRLGIPETE
                     TVVCGMALGFPDPDAKVNSFVTDREPLEVFVRWVDKLRT"
     assembly_gap    10683..10713
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10714..10963)
                     /locus_tag="PK264_01640"
     CDS             complement(<10714..10963)
                     /locus_tag="PK264_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRD74632.1"
                     /translation="MSKPVTTATRPITVEEAIRGRRSTRAFLKDQVPRELVARILETA
                     NRAPSGSNIQPWKVWVVDGEVKQQISKEMLETFFTAGEG"
     gene            complement(11036..11962)
                     /gene="tsf"
                     /locus_tag="PK264_01645"
     CDS             complement(11036..11962)
                     /gene="tsf"
                     /locus_tag="PK264_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRD74633.1"
                     /translation="MSNITAGMVKELRDKTGAGMMDCKTALTETKGDIEAAVDWLRKK
                     GLSKAAKKADRVAADGLIGVKVAGLAGALVEVNSETDFVARNPEFQTLVADIASVAPS
                     AKGDLAKLLAAKYPGKSLSVEQQVTELVVKIGEKMSVRRTGALEVKSGCIAGYMHNKV
                     ADGLGKIGVLVALESTAKDDKLIEIGRQIAMHVAARNPVALDLAGVPADIIAREKAIL
                     MEKNKDKPAHLLEKIAEGGLKAYAKENCLLEQVFVLDEKKSVGQALKDVEKAAGTPIK
                     LAGFLRYALGEGIEKKTGDFAAEVAAAVSGTR"
     gene            complement(12155..13171)
                     /locus_tag="PK264_01650"
     CDS             complement(12155..13171)
                     /locus_tag="PK264_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRD74634.1"
                     /translation="MALPAFNMRQLLEAGVHFGHQSHRWNPKMSPFIFGARNNIHIVD
                     LTQTVPLLHQALVKISDTVAKGGRVLFVGTKRQASDAIKDSASRAAQYYINHRWLGGT
                     LTNWKTISQSIRRLRQLDEILAGETQGRTKKELLQLTREREKLNQSLGGIKDMGFLPD
                     LLFVIDVNKEQIAIAEARKLGIPIVAIVDTNCDPDGIDIPVPGNDDAGRAITLYCDLA
                     AKAAIDGLERQQTSGGVDLGASETPPVEELPVVAEAGEAKGAFKGIDAPRGEADDLRK
                     LPGVGPKMAQKLNDLGVYHFWQIADLDASGLATLDKAMGLRGAAERNDWIGVAKKLND
                     EKAV"
     assembly_gap    13202..13214
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13498..13755
                     /locus_tag="PK264_01655"
     CDS             13498..13755
                     /locus_tag="PK264_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74635.1"
                     /translation="MQGTEASGAAVVEAKLRAIDDMIGQFLVHYPGAKPRHPGLGLVA
                     HPVTGTPFILLDDYDDDELRLHFIVHAHANLDDLDPAATEC"
     gene            13907..14143
                     /locus_tag="PK264_01660"
     CDS             13907..14143
                     /locus_tag="PK264_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRD74636.1"
                     /translation="MGSMSVWHWLIVLAVVLIAFGGSGRISSIMGDVAKGIKSFKKGM
                     QDDDEPKKPDPAKPNDQIMAGKPGEAPRVENKVG"
     gene            14369..>14636
                     /gene="tatB"
                     /locus_tag="PK264_01665"
     CDS             14369..>14636
                     /gene="tatB"
                     /locus_tag="PK264_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HRD74637.1"
                     /translation="MFEYGSVKLLLLAIVALVVVGPKEFPVLLRTIGKYVGMIRQQAA
                     EFRQQWDDAVRDSEITSLKNEVEKVGQDLQTTLDETRSQIDADLH"
     assembly_gap    14637..14724
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14774..15637
                     /gene="tatC"
                     /locus_tag="PK264_01670"
     CDS             14774..15637
                     /gene="tatC"
                     /locus_tag="PK264_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRD74638.1"
                     /translation="MQDATPTKLPGDEPKRDGHDEIEASKAPLLDHLIELRKRLIYSL
                     IGIGIAFVVCFYFANQIYQVLVQPYVSVRGDKAKMIFTAPQEFFFTKLKLALFGAVCI
                     AFPVIANQIYKFVAPGLYKNERQAFLPYLVMTPILFIVGALVVHKIVMPMMLQFFIGD
                     EIKTANLEITLEARVSEYLSLIMTLILGFGVCFQLPVVLSLLARAGLIDVAGLRSFRR
                     YAIVAITAVAAVLSPPDPFSMIAMMLPTVLLYEASILMIEHDIVGKFGNILDKFRRKP
                     PAPPASTTPTG"
     gene            complement(15699..16892)
                     /locus_tag="PK264_01675"
     CDS             complement(15699..16892)
                     /locus_tag="PK264_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74639.1"
                     /translation="MTIALVMAMTLASGDCRAQSSRPAGFEVALGPGWIEDMVRHPGG
                     GVLVARRGIKETGAKLSILHIDDGGALKWEKTFEDGGIVEVGGLSLTPQDRIVVAGFG
                     LATRGSTLWIKELSLAGEVLAESDVPAGGRAGAPLAFLGPSSFVHGTAINVITGQLEQ
                     SIYVTGTDGRSLARWHVPGMNLGSGVLVASRPDRKTAFVALDHSIRDADAGVGDSIPF
                     VALVDDQLRQMWTLRIAADTEEQPNVSAVGLVADGGVALAGLFNSNKRAEPGWVAVVD
                     AEGKQVWSRRFGRGGLALPRALVWLGNDASLAIGGCDASDFKGAPDTRRPLLIRIGAG
                     GRSEQSIELKGILGGRGFIRSLVSLDDGRLAAAGVSGAGCGEFDGLMSGTDTWIVVLD
                     SDAVK"
     gene            complement(16994..18628)
                     /locus_tag="PK264_01680"
     CDS             complement(16994..18628)
                     /locus_tag="PK264_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HRD74640.1"
                     /translation="MTMKTADAISAILKKEGVEWVIGYPVNHVLEHAAKVDIRPIIVR
                     QERTGLHMADAISRVTSGRKIGVFAMQHGPGTENAYGGVAQAWSESVPLLVLAQGYAR
                     RLAWVDRNYNASVSMRSITKSAEPITAGKEIPAIFRRAFQKLKSGRGGPVLVEIPTDS
                     YAEECDPSLYTSPAYIRTGTDLAAVREAAKMLVTAKRPVLYAGQGVHWAEAWAELKTL
                     AELLAIPVTTSLEGKSAFPETHPLSLGSGGLAIAQTVRTFLDKSDLILGIGCSFTETN
                     FGVKMPAGARIIHATLDPSDINKDVPVALGVVGDAKIVLQALIEEVQKLHGKSPKDAK
                     AVAAEIKAAYEPWLAKWLPKLTHDAAPLNPYRVLWDLQKTVDIDNTIITHDAGSPRDQ
                     LSPFWRATTPLSYLGWGKSTQLGYGLGLAMGAKLAKPDKLCINVWGDAAIGFTGMDFE
                     TAVRERIPILSILLNNFSMAIELKVMPVSTEKYRSTDISGDYAAFARAMGGYGERIEK
                     PADIVPAIKRGIAATKKGQPALLEFITSKEVEVSRL"
     gene            18813..19883
                     /locus_tag="PK264_01685"
     CDS             18813..19883
                     /locus_tag="PK264_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD74641.1"
                     /translation="MSSTLGASGKTTAGNFFEDFRLGQVFEHATPRTVTSGDVALYQA
                     LYGSRFALQSSDEFARGLGLPRAPVDDLLVFHIVFGKTVPDISLNAVANLGYADCRFM
                     ESVYPGDTLATTSEVIGLKENSNHETGVVYVRSTGTNQHGRPVLIYCRWVMVRKRDML
                     AAVAAAHVPALPERVDPAKLAATFPAWSTTAAAWARSGTGARFADYKAGERIDHVDGM
                     TIEEAEHQMATRLYQNTAKVHFNQHTEGQGRFKRRLVYGGVVISLARALSFNGLANAG
                     RIVAINGGRHVAPAFAGDTVYAWSEVVETAPLPSRRDAGLLRLRTFATKNRPCADFPG
                     RAEKGHADGVILELDYWVAVPT"
     gene            20032..20991
                     /locus_tag="PK264_01690"
     CDS             20032..20991
                     /locus_tag="PK264_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD74642.1"
                     /translation="MLELSRRDLIVSGAAATAMFGLKGPVAFIDAAHAQRAADSFKYK
                     VGDIEVFSLHEGQVERTLDAGFVRNTALDDVKKALTAASVGPDKFPNYFTVTGARIDG
                     KLVLFDVGFGGNGQLTVGRMAENMKAAGLDPTTVSTVVISHFHGDHISGLWTKETNAP
                     VFPNAEIMMPETEYKHWTNPALVEKVPEAQRNFVRRIQSTFPTWKNIKLFADNADVLP
                     GVKAIPTHGHTPGHTAFLVSSGSQQLFIQGDVSGMHQLFVRNPGFHSMFDADGPKAEA
                     TRRAFYDRVIAEKGMIAGYHFGFPNVGTLAKDGNGYAFTAVKA"
     gene            complement(21067..21408)
                     /locus_tag="PK264_01695"
     CDS             complement(21067..21408)
                     /locus_tag="PK264_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74643.1"
                     /translation="MKFAFSKFAIAAVLVGITIAQSEAKPISDLIKGGYKCEKVTLEF
                     SICEKDGEKWYCDSTGNCERVKRVPSRPGQVAPGPRPIFAPLKPRPPTWKPANPGRPA
                     KPPIETRSLDR"
     gene            complement(21734..22162)
                     /locus_tag="PK264_01700"
     CDS             complement(21734..22162)
                     /locus_tag="PK264_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRD74644.1"
                     /translation="MTAYDDSNVFAKILRGELPAERVFEDAATLAFMDIMPRARGHAL
                     VIPKAPVRSILDASPEQLAAVMRTVQRVARAQRVAYSADGTTIQQFNEAAGGQVVFHL
                     HVHVLPRWDGQRLQPHTGQMEKREVLAENAKALRAALAAL"
     gene            complement(22247..22666)
                     /locus_tag="PK264_01705"
     CDS             complement(22247..22666)
                     /locus_tag="PK264_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HRD74645.1"
                     /translation="MAFAGINYLAILIAAGASFLFGGVWYGILSQQWMAAVGKSEADI
                     KSGTSMPLLFGLTIIAQLIMAWVLAGLIGHLGQGQVTLRNGMISAAFCWFGFVLTTLV
                     VNHGYQGSRRALTVIDGLHWLGVLLIQGAIIGWMGVR"
     gene            complement(22698..>24032)
                     /locus_tag="PK264_01710"
     CDS             complement(22698..>24032)
                     /locus_tag="PK264_01710"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRD74646.1"
                     /translation="NRVAPASREAALLSFAPIVRKSAPAVVNVFVSARVEVRHPAMDD
                     PIFRHFFSERFGMPRERVQNSLGSGVLVSPDGVLVTNYHVIKGAKSEVRIVTSDRREF
                     EARLLLADEKTDIAILKIDNGATSFAFLELADSDGIEVGDLVLAIGNPFGVGQTVTHG
                     IVSAIARTEVGQSEAQVFIQTDAAINPGNSGGALVDMSGRLVGINTSIYSRSGGSHGI
                     GFAIPSNLARVYIESAMSGRRVERPWLGARLEPVTSELAKSLGLARIAGAHVKRVHQD
                     SSAAAAGLQAGDVITAIDGTEVLDPRSLLYRLTTVGVGATVRLDYLRKGRSRSTELQL
                     RGAPKPSKDDHRILAGPHPLDGATVATLTPARADELSLETEVGVVVTAVKPASIAAGH
                     GFRVGDILVDIGRSAVASIADVETRLATAPALWQIAVKRGARVLTLRVPGTR"
     assembly_gap    24033..24113
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24138..>24506)
                     /locus_tag="PK264_01715"
     CDS             complement(24138..>24506)
                     /locus_tag="PK264_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74647.1"
                     /translation="SEPVMAPLPTGSSCVDDSKRCVDERSLALKALMADKDKRWLREP
                     STPAAHASGVRLFAMQQRKRELTCEELALAKREADRAPAELRGPGGAGLAPGQVSRGV
                     ILAGEVGRELMGEMKRRCRV"
     assembly_gap    24507..24553
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24744..25698
                     /locus_tag="PK264_01720"
                     /pseudo
     CDS             24744..25698
                     /locus_tag="PK264_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004689000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
     gene            complement(26104..26617)
                     /locus_tag="PK264_01725"
                     /pseudo
     CDS             complement(26104..26617)
                     /locus_tag="PK264_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611490.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            26793..>29085
                     /locus_tag="PK264_01730"
     CDS             26793..>29085
                     /locus_tag="PK264_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD74648.1"
                     /translation="MSRIERIPHGSHWGAYWLLEAGRIVGVEPFEGDPDPSPIIKSVA
                     AWADPHRRVMQPMARKSWLAARRAGRLATVDERARRGRDTFTPISWDEALSLTANEIR
                     RVIADHGNRSIFAGSYGWTSSGRFHHASTLLRRTLNLVGGFTGHADTYSIAAGPVILR
                     HTLGNTDACQGRANTLENVAKNAETLVVFGAMATRTAQSEAGGIAKHRLAENLRAIAE
                     RGVRVILVSPQRDDLPDWLGAEWWPIKPNTDTALMLALAQEIVGTGRHDAEFLARHCS
                     GSELYLDYLSGTTDGTPKTAEWAAAITGLEPAQIRALADTLVRTRSMLSLSWSLQRAS
                     HGEQPFWSALGLAAVAGQIGKPGGGVAYGYGSLGGVGGPVTLSKTPAMSQLSNDLDSF
                     IPVARVTDMLESPGKAYDYEGKTRHYPDIRMVYWAGGNPYHHHQDLNRLERAWALPET
                     IVVQDPMWTPTALRADIVLPATTSIERNDLAGNRRSDTIVAMRKAIDPIGEARSDFDI
                     FSGLAAKLGVRERFTEGRDEMAWVRHLYEATRQDAKERCNFDMPDFDTFWEAGTAAIP
                     TITDQTHLAAYRADPEKHVLATESGRIVLGSALLTAKNYADCRPHPAWLPPAEWLDPA
                     AEKAGAFHMLTPQPDGRLHSQLVHAGPSHARMPDGRERLRINPVDAKRLDLKKGDVAR
                     VWNGRGACLAVAEPYDGIRQGVVALPTGAWLTPGGDAGAPELAGNPNVLTPDIPTSSL
                     GQGCAAQTCLVHVERWRGNDSVAA"
     assembly_gap    29086..29098
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            29577..30122
                     /locus_tag="PK264_01735"
     CDS             29577..30122
                     /locus_tag="PK264_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74649.1"
                     /translation="MPSINWSLYLGHFQTALLYGLVPIAVMVVLLHGLLWASTFRRTD
                     EHRAILQFHCLPFTALGVLTGMFTGASRTAVVGVIVPPVLTFISGLAAYVLGKDAKPI
                     WRRIVPLAIAMMTFGTMFGAAFGASMRQANEERDRQLEREKLRYEKVELPFMQKAVEK
                     GGSTPAPASANPGAVPSVSIK"
     gene            complement(30166..31389)
                     /locus_tag="PK264_01740"
     CDS             complement(30166..31389)
                     /locus_tag="PK264_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD74650.1"
                     /translation="MKSTGRSRAIAIIAVAEVLTLALWFSATAVVPSLRATGAISALQ
                     QSLFTSAVQLGFGAGSVASAMLGLADRLEPRRFFAASALVGAIANGLVAMLDPSSPMV
                     ILLRFITGATLAGIYPVGMKLAASWAGATGRQDMGFLVGLLVGALTLGSASPHLFNAF
                     GFHSWQAMMIAASLSATLGAGLILAAEVGPRFSKGGAFRRDLVGQALADRPTRLAILG
                     YLGHMWELYAMWAWIGAFLAASFAAGLAPANPQRSAALATFAVIGIGAIGCFGAGWLA
                     DRIGRTAVTSIAMGVSGACCLVVGLLFDGPLVLLLMLLMVWGVAIVADSAQFSTAVAE
                     LTEPAVVGSVLTLQTASGFLLTLVTIHMMPYAVSWLSWRWAFATLAIGPFLGVVAMLR
                     LRSRPEARRLAGGRR"
     gene            complement(31389..32156)
                     /locus_tag="PK264_01745"
     CDS             complement(31389..32156)
                     /locus_tag="PK264_01745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRD74651.1"
                     /translation="MRIDLNADMGEGFGPWQMGDDDAMLEIISSANVACGWHAGDPNI
                     MFRIAEKAKAKGIALGAHPSFNDLWGFGRRVIRGDSMAEIERMVAYQIGAMQGMASLA
                     GHKVTFVKAHGALGNLTNAEPEFAEAVGRAIKGVDRSLVYVAMPGLPTERIAEKLGLK
                     AVMEAFADRTYEDDGQLTPRKIAGSVIDDPVIAAERALQMARDQVIVARSGKRIKARI
                     ETICVHGDKPSAVAQARRVRDVLEGAGMKVQPFAPAA"
     gene            complement(32251..33903)
                     /locus_tag="PK264_01750"
     CDS             complement(32251..33903)
                     /locus_tag="PK264_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD74652.1"
                     /translation="MKPLDAVSLEVIRNALVAAAEEMGVTIWRTSRSSVVRDLLDYST
                     CMFDADGKSVAQATCIPVHLNSMSSCLDDILARHIPLKDWREGDIIITNDPYAGGQHL
                     NDILTFKPAFVDGRLVGIAGVLVHHLDVGGGAPGSYYAAATEIYQEGFRIPPMRLDNA
                     GRRNTEVIALLLRNSREPANVGGDFASQLAALDIGVKGLHRIAKRYGADRLQDACRGI
                     RRQSEMAMRRAIAAIPDGTYSFEDFVDDDGIDRETRLRIAVALRIKGDALTIDMTGSS
                     PQAQGPVNCTRNMSASGAFCGLLMSIGSDIPANAGVYEPVTIVAPEGSCVNARSPAPV
                     ANRMAVGHRVCNTVMGAFSRAVPGKVPAAYYGVSYAFATNLFHADGRRQVYFDLTCGG
                     WGGHPEGDGANGFSCGLHNIANAPVEMLENTYPITFQRYSLAPDSGGPGRHRGGCGLE
                     RSFRIDAPAGTLSLNLDRFKTRPYGIEGGAPGAAGFATLTRADGRTEQLPSKLAGMRI
                     GKGDVFSLVTAGGGGYGPPGERDPVAIASDIENGYVSRRSER"
     gene            complement(33900..>34595)
                     /locus_tag="PK264_01755"
     CDS             complement(33900..>34595)
                     /locus_tag="PK264_01755"
                     /inference="COORDINATES: protein motif:HMM:NF039581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD74653.1"
                     /translation="GASGLHACETADEIGITRVVFPRDPGTLSAWGMLYSDIVHALAR
                     ARLMKADAGSLPQLSAIVAELDAAGRQMLTRDGVAAEAQQLPVFLDMRYPGQAYEIQV
                     PLETPARLAEAVARFHERHLAQYAHAEPHVIPDIVTVRIAATGRLAKPIEHTPPIAKG
                     DPAKGRRRVLVGEGWHDCPVLDRARLASDSRNAGPLIVEEEHATHLVPPGWSLVVAPT
                     GDLVATKDSGDRT"
     assembly_gap    34596..34653
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34654..35956)
                     /locus_tag="PK264_01760"
     CDS             complement(<34654..35956)
                     /locus_tag="PK264_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD74654.1"
                     /translation="MKPKAKRRSFRVAVDIGGTFTDLQVLEEATGQTHAAKTPTTPDD
                     PSEGLIAGLKLASERIGFSLADVSALMHGTTIATNAVLQRRLPKGALITTHGFEDVLE
                     IGRHFRRDVYASKAEPRAILIPRALRLTVRERMRATGGVETPLDEADVAAIAERLKAS
                     GVRAVAVALLHAYANPAHERRVGELLARLAPGLDVSLSHEVSPEIREFERASTTVLNA
                     LLMPVVRGYLEKLRTRLAAAGFKAPVYLVQSNGGVTTPETAAVQPARLLLSGPSGGAL
                     AGETIARRLAMPNMVAVDMGGTSYDVSIVNEGAIRLVTEGVVDGLPVRLPMIEIRTIG
                     SGGGSIARAEAGGRMRVGPESAGAKPGPVSYRRGGTEPTVTDANVVMGRIDPGYFLGG
                     AMSLDAEGATAAVRTKVATPLSLSLDDAALGIARVATSRMAG"
     gene            36156..37337
                     /locus_tag="PK264_01765"
     CDS             36156..37337
                     /locus_tag="PK264_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRD74655.1"
                     /translation="MPKAATSPAEQRVLDWLARHEREMIGLIEEVVNIDSGSYDKAGV
                     DRVGERFIRFFEGRGMIARREAHASFGDAIHVRLDDTPTNERPVVLMGHRDTVFPKGE
                     AGRRPFRIEGGRAYGPGVSDMKAGLVMNAFVLAAFKELGGAPAPLAGLITSDEEIASP
                     SSRPVIERVAREARCVFNSEPGRPTGAVVTGRKGGVFMRMVIEGRAAHSGGNFEYGIS
                     AIGELAHKIVAIHALTDLKRGVTLNVGLVKGGQSVNTTAPEAEAQIDFRYVTPKDRAE
                     ILARLEAIVAASTVPGTKATLTISGEFLPLNATPSSEELFGGYKAASADLGLTVAGEF
                     TGGCADSGYTAGVGCPTLCAVGPVGGKAHTAEEYLEVASIVPRAQALALAILRLSPDK
                     R"
     gene            37364..>38838
                     /locus_tag="PK264_01770"
     CDS             37364..>38838
                     /locus_tag="PK264_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74656.1"
                     /translation="MNDATSVAAEDQYVTHAELAPEEGQPNALDNHRLHELILRLQSE
                     NRHLAAITVRGWSITNPIELGLHGTRGLPLPRLSFVSCSNLKLSARFARLNALSLNAC
                     AIEQLDLTDAVIDGPLDLEHSTITGEHDTTLALTGIKAQSILITRLSRSDENFYLSID
                     LGAARIERGLVIDAVRLKGPLMLDRTSLGADATITGSRFERRMGAAVSARALVTGGPL
                     SFSSCQADGAVNLADARIGGDLSFDHVEIRSTPSESVLLADTTVQGTVRVANSTCTGT
                     IQLRMCAISGSVAVEHTTCNPLLGAAISILGGKIGAEITLVGLKLSAQLEVRSVGIGL
                     NMVLDDVAITGDERFPINLLLNRIGGGLDIIKLRYAGYGTLRACSIGASLYLEEITLN
                     PRRIEDQRSLYDCNLLATENVVGGKLTLGTIASEGEDARGLVDLRNTRCASFGFLGHA
                     DSENIHINLNGLDYGKIDQLNLASAKFLRAMVDRNERRRYE"
     assembly_gap    38839..38925
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <38926..39511
                     /locus_tag="PK264_01775"
     CDS             <38926..39511
                     /locus_tag="PK264_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74657.1"
                     /translation="PPQPQAYMHLARVLTNEGLDEDAEKVMVSMRRSTRFAGAMRKPL
                     TYLISGLFDVCFGYGYSRWKALITLTLWICLGWWGVTQALAQGVMVVDVQAVTTGGGP
                     LARWATVSSPPEPPCKGQIEPLFYAIDLTIPVLDLGQVRKCTIRLDEAEDHLSVWQKS
                     TTRWWMLRYVYELLGGLIVSLTALTFTGTLKTKL"
     gene            complement(39519..39938)
                     /gene="arfB"
                     /locus_tag="PK264_01780"
     CDS             complement(39519..39938)
                     /gene="arfB"
                     /locus_tag="PK264_01780"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRD74658.1"
                     /translation="MRITATLSIDESELSEQYMRASGPGGQNVNKVASAVELRFDVRA
                     SRSLPEPVRVRLERLAGRRLTKDGVLVIRADRHRTQEMNRSDARERLRELIEVAATPP
                     KPRVKTRPTKASKERRLEGKARRSTVKSMRQSRPGDE"
     gene            40235..>40852
                     /locus_tag="PK264_01785"
     CDS             40235..>40852
                     /locus_tag="PK264_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74659.1"
                     /translation="MQDRTPASQAVAQPSAAALAVAHDDGPTTRATARDAAASPAAVP
                     RHGAPVLSIKDLHVHFVTTHGTVRAVEGVSYDVHAGEMVAIVGESGSGKSVSALTVMR
                     LLPPNTARIMNGSISFEGRELLKLSDEEMRQIRGRSVSMIFQEPMTSLNPVLTIGLQI
                     KEPLLIHLGMSEEQATTRAIELLTLVGITDPSSRLAQYPHQLSGGM"
BASE COUNT         6844 a        13617 c        12978 g         6814 t
ORIGIN      
        1 cgcctttctg ctgaggctca atgatcaggc agagggagag cgcgatggcc ggatcacggt
       61 tcgcagtgtt gccgcgcggc atgccagccc tgctgctggt cgcgttcgcc ggcgcggatc
      121 tggctgtcgc ctgtgcgccc ccgccgcccg cggtgaccac gatcgctctc ccgcgcttct
      181 acgcggacaa ggaggggagc gaggtcgacc cgaagctcaa agccgcacac gccgccgcan
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
      361 gctcgccgcc tgggccgatg ccggcgcgct cacgggccgc atggaaaccc gccaggccga
      421 atatcagcgc aagttcgatt tcgtcggcct cgcgctcgcc tgggtgaagg tgcgtccgca
      481 cgccagcgag gtggagcgcc gcgcgatcga cgcctggctg atccgcctcg ccgacctctc
      541 ccgcgccttc ttcgacgatc gcgggaacag ggtgccggcg gtgtagacgt ccgggccnnn
      601 nnnnnnnatc gcggccgcaa gcggaacaat cactggtact ggctcggcgc aggactcgcg
      661 gcgactgcga ccacgacgga tagcgagcgg cactggaaac tcgcgcgcga gatctacgcg
      721 gacgctgccc gcgagatcac agccgagggc ttcctgccga tggagctcga gcgaaaatcg
      781 cgggcgctct actatcatgc cttcgcgctg atgccgctcg tcacgatggc tgagctggcg
      841 tcggcacgcg gcgagaattg ggcgtccctc ggcgatgacg cgctgcatcg tctggcggaa
      901 cgcacgcgtg cggggcttct cgattccggc cccttcgaca agcgtgccgg cgccgcacaa
      961 gagcggccac tgaaacccgg cgccggttgg ctcgagctct acgcgatccg gcatgcggac
     1021 cgactgcaga gcccgagggt cgacatgccg cccggccacc gctggctcgg cggagatgtg
     1081 cggctcctgg cagaggctct gaagccgcgc gcgaactgac cgccgacgca agaaggccgc
     1141 ccgaaggcgg ccttccaaag tctcatctcg tggagcgatc ttcagacctc aatggaggcc
     1201 ccagatctcg accacggcct cgccgccgag gttcagccgc gagcgatagt tgatcgcgat
     1261 cgactcgatg acgcgctcgc gcccgcgcag atcgatcggc gaggacgttt cgccgccgcg
     1321 cagctcgcgg ttgaccggca gttcctcggt ctcgccgttg gcatacgtga cgcgcagcca
     1381 gttgacgcgc actgcgtgac ccttcgcggt cacgcggatg gcgcggaacg tgccccactg
     1441 gcgaccgacc tggaaggtgt cgacgtcatt cttgagcatc gcggcacgct gcgagccgag
     1501 cagaacccag ccgtcgttgt gcttcttggc gtcaccggag acccagctgg cggcatactc
     1561 gccgaacacc tcgaccgtgg cacggccttt gaagttcggc ttcgcctggt aggtcatgtt
     1621 gatctcgcgg atgaaccgcg taccgccgat gtcgagcgcc ggcgtcatcg agtttgcgcg
     1681 aatctcggca ttgatcggga agcgctgcgt ctcgccgttg ccgaacacga tctcgatgtc
     1741 acgcatcagg atgtcgttgc cggacacgcg gaaggcgatc ttgtcgaagc gcccgacctc
     1801 gcggccgacc gcgatcgtgt cacgctcggc cttgaagccg accgtctgcg tgccgaacag
     1861 cacccaacca cggggaacgg cggagagcgc cggaccgtaa gacgggcggg gcgggggcgg
     1921 cggaggggcg ccgtagcccg gcgggatggc gaagcggcgg ttgctgtcgc cgagcagctg
     1981 cagcgtcgcg ttgccgcgcg cgctcggttg cggtctgagg ctcacgatga cttcgcggat
     2041 gtgcgttgcc cgttcgccgg cgaggtcgat ctccgaggtc atctcaccgg gtgcgatcgc
     2101 ctgacggacg cgcatgtctt cggtgtagcc gttctcgtag cggatctgca cccggcggat
     2161 gaagagcggc tcatcgagcg cgcggaggcg gatcgccttg taggcgccct ccttccagcc
     2221 gaccggcatg cgtatctcgt ccttgcgcac gtcaaaggtc tgcacgtcga gctcggcgta
     2281 gcccgggggc acgcgatcct taacgcggac atgctgcagg ccccagacct cgacgaccgc
     2341 gcggccggcg tagcccgggc ggctgcggta aaccatgtcg atgcgctgga tggcgcgggc
     2401 ctcgcccttc aagtcgagca tcggcgtctc tgtgccggcg cggatcagct gattgaccgc
     2461 gaggtcctcc tgctggccat tgccgtacgt gaccttgagg tcgatcagga agacatcgga
     2521 cttccgcacg atcagcttga gcgccttgaa acggccctcg ctgcggccga cttcaatcac
     2581 atctcgatcg acgccgaagc cgacctcctt ctcgccgagc atagtccatt cgccgccggg
     2641 cggtcgcccg cgttgtgcct ctgtgggcat tacgctggtc gcaaccgcgg ccacaagcag
     2701 ggcaaaccct gccagccaat tccgcagtga cattctgttt gtcctccaac tgccgggcct
     2761 ggtgacacgc gccatcgagt ggcgatccca ggctgccgaa cccgaccact gcatgcgcgg
     2821 tgacgggccg aacgtgatcg gccaatcgct ccggcttcag ccgtcttctt gccgcgccac
     2881 gcaccattcg cacgtaggcc gatcaatcgc aaccaacgat cagaacgtgg gcggacacag
     2941 gaggacactc cataattccg ccggaagcga ggcagcctgg caatcgatca atgacaaaga
     3001 ggcccgcgag catggctcgc gggcccggat ccccgatcgg agatggatcc gtcggggcat
     3061 tgccccgctg ctgagatcgg cgatcagccg accttcttca gctgctccat ctggcggnnn
     3121 nnnnnnnnnn nnnnnnnnnn nncgaaggtc tgctgggcga tgcgggtcga aagctcgaag
     3181 agctccttcg cctggttgcc cgcaatcgcg aactggctct gaacaaaggt cgcctgcagg
     3241 cgggcggcct cgggaagcgt cttggccttc acgatggcct gggcggcgtc gaacatcgcc
     3301 tccgtgttgg cggctgtgtt ggcgagcatc ttctcaccga tgacgcgagc gccggcgtgc
     3361 gcggcgagga aaacttcctc ggcgaccttg gcgctgtcct tcgtggcgac ggccatgcgg
     3421 gcgtacgcct cgcgggtctt ggcgaccgta tcctcggcga tctgctgcac accctcgggg
     3481 atgcgggcct cggaggcggc cttcatgaaa tcgnnnnnnn nnnnnnnnnn nnnnnnnnnc
     3541 ggctgtcctt tcgctcgctc gcgctggaaa ctcatcgttg ccggccggga actagtcaac
     3601 acggtggccg cgctcgatgt gtgcttgtgt atagtcccag tcattgtgca atgcaacatg
     3661 attgtgcaat gcaaaattgc aaccttcgtc taacgacttg acatggatgc ccccgagtgg
     3721 tgcgtcctgt cacaaatctg aggcccttcc ccggagtttc gacatgcacc agacggcacc
     3781 ctactggtgg gatgcggcgc cacccgccat gctgccgacc tcgccggttc gacctaagtg
     3841 tgacgtcgtc atcgtcggcg cgggctacac cggcctcagc gccgccatca cgctcgcgcg
     3901 cgcaggccgc tcggtgcagg tgttcgacaa gatgcgtccc ggcgaagggg cctcgacacg
     3961 caacggcggc atcgcttccg gcaatctgcg cccctcgatg aaggagctga caaagacctt
     4021 cggcgaggcc cgcgccctcg gcatccaggc cgagtccaaa gccgcgcgcg acgatctcgc
     4081 tgccttcatc cagcgcgagc acatcgactg cgacttcaag atcgccgggc gcttcacggg
     4141 ggcctcatcg cctgccgcct acgacgacct cgcccgcgac gccgagatgc tcacccgcac
     4201 gctcggcatc gaggcgcgcg ccgtcgcaaa gtccgaacag catcagtatc ttggcacgga
     4261 cttctatcac ggcggcctct atcgcaacga catcggcggc ctgcatccag cgaagtttca
     4321 cgccggcatg ctgcgcgtcg ccatcgctgc cggagccgtg atccacggcg agacggcggt
     4381 cctcggaatg cgccgcgagg gcgatcgcca cgaggtcgca acggcccgcg gcacggtcag
     4441 cgccgaccag gtgatcgtcg gcacgaacgg ttacacggat aaggccgata catggctgcg
     4501 ccggcgcctc gtccccgtgc gcagccgcat catcgcgacc gagccgctct ccaacaacct
     4561 gatgcgcgag ctgatgccca agggcgtcat gtgctccgag acgcgcaagt tgcactacta
     4621 ctaccgcccg tcgccggatg gtacgcgcat cctgttcggc ggccgagacg gcaccatcac
     4681 cggcgacagc ccccgtccga ccgagagcct ccgccgcgcg ctcgccgaca tcttcccggt
     4741 gctctccggc acgccgatca cgcacagctg gttcggctac gtcgccatga accgcgacat
     4801 ggtcccgcgc atcttctcgc gctccggcat gcagtacgcg accggttact gcggctcggg
     4861 cgtggtgtgg gcgcgctggg ccggccagaa ggccgcgctg caggtgctcg gcaatgccga
     4921 tggccagtcg tcgctcgatt tccgtccgcc gggcatggtg cccgtggtcg gcggcaaggc
     4981 ttggttcatg cccggcgtct tcgcctggct gacgctccag gacaagctcg cagcccgcaa
     5041 gcgcaagcag acgggttgac gggtcagcgg aatgcggcct ctgttgacgg tgatgggtga
     5101 aatgagcgcg attcgatgtc gaaaacatcg cgctcggccc gaccatcgcc atgttcgtcc
     5161 cgacaggtga gccgtgtgac cgacaagccg gaccaaaacg acagcaggat cgcgcccgat
     5221 ccgatcgcgg ccgtgcttcc cgcgctggca gctctcggca cgatcgcgtc gatcgccacc
     5281 gtcaattggg tcgcccacga caagacgcca gatcgcacgc ggatgcgccg caaggcccag
     5341 acagcgctcc gcgatctcga atcatgctgc atggggctgc aggagatctt caagcggctc
     5401 taccgcaatc cgaagctatt cgcaggcgag ggcggtgccg cctcctcgcc tatgaagttc
     5461 ggcgtgcacg ggccgcgcat cgatcccaca ctcgcgcgca ccttccacca gctcatgaac
     5521 gatatcgcga gcatgctcgt gctcgcctcg cagaacgcct acgacgtcat gtgcgccgtc
     5581 gaggacggcg agatcatcgc gcccgaggag ctcttcttcg gattcggcga gcagcaggag
     5641 cggctgaaca agcttttcac ccagcgcgcc accctgcgcc tctcggtcga gaccggcctc
     5701 gacatcgccg accgcctcac cgcgctcgtg cgcgaactca agcgcttcaa gatcgactga
     5761 cggccacacc cgattttcgg accgagactg cgacagccgg aggaggctca catgcgcttc
     5821 atgaacgtga cgcttgacga cgatgatgta cctcagctcg ccctcgtcga ggggaacaag
     5881 gtgcgcctgc tcggcgacgc tgccgatatg gatgtcccca tgtcgctcga cgagctcatc
     5941 atgatggacc cgcgcctcga gagcttccag gagatcctgc gcgagaaccc ggacctgccg
     6001 acggtgccgc tcgagaaggt gcgcgccgac ctgccactcg acttcatcga caaggtcgtc
     6061 tgcctcggcc tcaactacct cgagcacgcc aaggagggcg gccacgcccc gccgacgtac
     6121 ccctccttct tcctgcgcgc gccgagctcg ctcgtggcgc atggccaacc ggtgatccgt
     6181 ccgaaggtct ccgagcagct cgacttcgag tgcgagctcg tcatcgtcat cggcgaggag
     6241 ggccggcaca tcgccgagaa gaatgcgctc aagcacgtct tcggctacac gctcatgaat
     6301 gacgtgtcgg tgcgcgacta tcagcgcaag accacgcaat ggaccgcggg caagaatttc
     6361 gacggcaccg gcccgctcgg tcccatggtc gtgacggcgg acgagctgcc gcccggtgcc
     6421 aagggcctca ggatccagac gcgcctcaat ggcaaggtca tgcaggacgc gaatacgtcc
     6481 gacatgattt tcgacgtcgc caagaccatc tcgctcctct ccgagatcat gacgctgcgc
     6541 ccgggtgatc tcattgcgac cggcacgcca tcaggcgtcg gccatgcgag gaagccaccc
     6601 gtgtggatga agcccggcga cgtggtcgag gtcgaggtcg agaagattgg cgtgctgcgc
     6661 aatcccgtga tcgccgaagg cgacgagccc gacgcctgaa gccgacggcc gctccagctg
     6721 gcgccggtcg tcgacactgc tcccttcccc ctcgacgggg gaagagctga gaatgggggt
     6781 ggtgcacgac aagatggggg tagtgcacga cgggccgggg cgctgcacga taaacaatgc
     6841 tgcgtacccc ccaccctcga tccctccccc gcaaggggta gggaagcgaa gtcgagggtc
     6901 ctcgatagcc ggtcagagga atggttgccg cgctaaagac ttatcgtgct cgctctggcc
     6961 cttcacgctg gcttggcaag cttggcgagg tcgtcggtcg cctccttcag tgccttcgca
     7021 tatcggtcgg ccgcctcgtc ggcctcggcc ttggtcatgg agagcggcgg cgagaacgag
     7081 gtcacctcga tgaacggcag cgcgcgcgag agcacgccat gctcgaaggc cttggccgag
     7141 accagtcggt gcacgcccgc cttcggatcg aagaagcgct tcttgccctt gtccgccacg
     7201 aactctgccg cgatcatctg gccaatgccg cgcacctcgc cgacgaaggg gtgatcgcca
     7261 atgcgctcgc gcaggccctt gaggaaataa ggcccgacct cggccgcctg ctcgacgagc
     7321 cgctcgcgct ccatgatcga aatgttgacg aggccgagcg ctgcaccgac cggatggccc
     7381 gagtaggtga agccgtgcat caccgggccg tactcgggag aggccttcgc cagcacgtcc
     7441 cagaccttcg cggcgattac cgaggctgaa accgggaagt acgcggatgt gatgcccttt
     7501 gcgagggtca cgatatcggg cttcagcttc atgccgccgg tcgagaacca gtgcccggtg
     7561 cggccgaagc ccgtgatcac ctcgtcgacg acgaacagga tgtcgtgctt agcgagaacc
     7621 ttctgcacct tctcgaagta gcccttcggc gggatcagca cgccgcccgt gcccatcacc
     7681 ggctcggcga tgaaggcgcc gatcgtgtcc gcgccctcgc gcgcgatcac gccctcgatc
     7741 tcggcgacga ggcggtcgca gtactgctcc tccgtctcgc ccggattgcc gtagcggaaa
     7801 tagtgcgggc aggagacgtg cacgacgtcg cccatcggca ggtcccaggc cttgtgatag
     7861 gcggggatgc cggtgaggct cgccgcggcg aaggtgaggc cgtggtagcc gccgaggcgc
     7921 gcaatgaact tcttcttctg cggcttcccg cgcagcgcat tgtaaaggcg cacgagcttg
     7981 tagttcgtat cgttggcgtc cgagccgccc atgccgaaga acaccttcga catctgcggc
     8041 gcgtcggccc gctcgtggaa gagcttcagc accttctcgg cgagccggat cgccggctcg
     8101 ttggaggcac cgccgaagat gtgatgatag gcgaggctct cgacagcctt cttcgcagcc
     8161 tcggccatct cggggcggcc atagccgata ttgacgcacc agagtccggc gccgcaatcg
     8221 atgatgtcac ggcccttctg gtcgcggatg cgcacaccgc gcccatccga atagatgacc
     8281 ggccccttct tcatgtggtc ggttatcgac gtgttggggt ggaacacgca ctcgcggtcg
     8341 atgtcctcga gcgggaaatt gcgctgcgcc tcatccatcg gcatggccgg ctccttgacg
     8401 tgcgccgtca cgggcgacgg caggatcgga cgaacttatg ggcgcgcgcc ccgcgcgtac
     8461 agcgaaatga cgctctgcgg gccagtcatg tccgggccgt ggacgggtca gacggcacgt
     8521 cgagcacgag gcagccgacc ctcgaggggt gatcgaggac gaggctttgc gctgccttgc
     8581 ctctgagtag ccacagcacc aggaggtcgc cgcccgctgc aatcgtcatg aggatggcca
     8641 cgctcatgag cgcaccatgc cccgtcgcga tcgccacgaa cgcggggagg agaccaagca
     8701 cgaccccagg catcatgatg ccgatgcgat agtcacgcgc cggcagaggc accaaggcgt
     8761 tggcgtaggg cgaaagcgtc gtccacttga agccgtagcg cacgacgcgc cgaggttggc
     8821 gcgccagcag cgtccacgtc acgccgtgca acagctcgtg cgcgatgatg ccgacgagga
     8881 agacggcaag aaatgtgagc gggcccagtg cggcgccgag gacgagacgg tcgaacgtcc
     8941 acacgacgga gaaaacccag ccgagcaggg ccgtgatcgg gagaataccg agaaggccgg
     9001 caagattggc catcgcgaaa gagacggaac agtcatgcgc gcgctcccat ccgggcggca
     9061 cgatcacatt cggggctggc gtggttgggt ctggcccggg cgcggcggaa gggtctgtca
     9121 cggcaggccc ctgagcgctc gaggctcatg aaaatcgatc aagtgaatgc ggcattcgag
     9181 agaggagagg aagcgagaga ccggcctggc aatcgccggc caggtctctc gcacctcaga
     9241 tcaggccgcc ttgatgttgg cgacggcgat cttgcccgag cggcgatcgg tcgccgtgtc
     9301 gaacattacc ttctgacctt cggtcaacgg tcccatgcca gcccgctcga gagcggtggc
     9361 gtgaacgaac acgtccttgc cgccgtcgtc aggcttgatg aagccgtagc ccttttggtc
     9421 gttgtagaac ttgacagtac cgccttgcat tttgtccatc tccggaactc gttcagaaca
     9481 ccggttcgtc ctggcaaacg gccggtgatc gattcttggg tggaggcccg agatcatgcg
     9541 ccggcgacat gcgctgaggc ggatggcccg atagtccggc caaaaaaatc gatcgccgtt
     9601 cctaagccgc ttctgcgtcc actgcaagac ggatcgttga aggattttcg agtttgattg
     9661 cggatgcccc aaaaagaggc tctggcgatg ggccgtgccg atgctaagtt aaccaggccg
     9721 aagcgcgccg ccgagatggt gccaggcctg agatcgtgag ccctgtcaac gccgtatgaa
     9781 accgccatct cccttgatcc gccgtggctt gacagtcggc ctcggccaca acggcgggcc
     9841 gccgctcgac gagccggtca acgacctctt cgtgcgctac cgctggcgaa aggcccacaa
     9901 ggaggcctgg aagaacccgt cgcactcgat catgatgttt cgattggcgc gcgcccgcgc
     9961 ggccggcgtc acctatgagg agtacgtctc gacgctcctc gataccggcc gccacctgca
    10021 ggccggcgat cgtccagacg gggaaaaaac cgatatttcg cagcaagtta gaggcgctac
    10081 ggtcctcccc gtcggaccgg cgccagcgtc ggcaaagccc agaggtgcgc gccgcctcgc
    10141 ggccaagccg gtatcgctga cgactggcca tcccgatcat cgcagcgctg ccccgtcagt
    10201 gccagggccg cgcaagcccg ctcgcccgcc tgcaaagcct gcgccgctgg ccgaagctac
    10261 gtccgaagct tatcgaccca acgcacgaaa acctcgagcg gctcacgatc cgtgacgaac
    10321 gagttcacct tggcatcggg atcaggaaag ccgagcgcca tgccgcagac gacggtctcc
    10381 gtctccggaa ttcccaagcg cttcatcagg atcgtatgga agttgcagag cgccgcctgt
    10441 gggcacgtct cgaggccaaa ctcgcgcgcc gcgatcatga tcgactggat gaacatgccc
    10501 gtgtcgagcc acgagccgag ctccatgtcc ttgtcgatcg tgaagatgag acccgtcggc
    10561 gcaccgaaga aatcgtagtt ctcggcgcgc tgcttcttca ttccgtcctt gtcgtcgcgc
    10621 gtgatgccga gcgtgctgta gagaccccag ccacaggcgc ggcggcgcgc gagatatggc
    10681 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgccctc gcccgccgtg aagaacgttt
    10741 cgagcatctc cttcgagatc tgctgcttga cctcgccgtc caccacccag accttccatg
    10801 gctggatgtt cgagcccgag ggggcgcggt tcgccgtctc gaggatgcgc gcgacgagtt
    10861 cgcgcggcac ttgatccttc agaaaggccc gcgtcgagcg gcgaccccgg atcgcctcct
    10921 cgaccgtgat cgggcgtgtg gcagtcgtca ccggcttgct catggtcgtc acctcggcgc
    10981 tcgggtccgc tattgaaaag gcccggcgct tggccgggcc tccgtgcatt cgaactcagc
    11041 gcgtgccact tacggccgcc gccacctcgg cggcgaagtc accggtcttc ttctcgatcc
    11101 cttcgccgag cgcatagcgc aggaagcccg cgagcttgat cggcgtgccc gccgccttct
    11161 cgacatcctt cagcgcctgg ccgaccgact tcttctcgtc gagcacgaac acctgctcga
    11221 ggaggcagtt ctccttggcg taggccttga ggccaccttc ggcgatcttc tcgagaaggt
    11281 gcgccggctt gtccttgttc ttctccatca ggatcgcctt ctcgcgggcg atgatgtcgg
    11341 ccggaacgcc ggcaaggtcg agcgccaccg gattgcgcgc tgccacgtgc atggcgatct
    11401 ggcggccgat ctcgatgagt ttgtcgtcct tggccgtgct ctcgagtgcc accagcacgc
    11461 cgatcttgcc gaggccgtcg gcgaccttgt tgtgcatgta gcccgcgatg cagcccgact
    11521 tgacctcgag cgcgccggtg cggcgtacgc tcatcttctc gccgatcttg acgacgagct
    11581 cggtcacctg ctgctcgacg gaaagcgact tgcccggata cttcgcggca agcagcttgg
    11641 cgaggtcgcc cttggccgac ggcgcaaccg aggcgatgtc agcgacgagc gtctggaact
    11701 cggggttccg cgcgacgaaa tcagtctcgg agttgacctc gacgagggca cctgcgagac
    11761 ccgcgacctt cacgccgatg aggccgtcgg ccgccacgcg gtcggccttc ttggcggctt
    11821 tcgagaggcc cttcttgcgc agccagtcga ccgctgcttc gatgtcgccc ttcgtctcgg
    11881 tgagggccgt cttgcaatcc atcatgcccg cgccggtctt gtcgcgcagc tctttcacca
    11941 tgcctgcagt gatgttgctc atgtcggcat cctttcgaga gccagagctc ggttactggt
    12001 caaaatagcc cgcctcggca cgccggcgtc caggtgtgcg cgttgcatgc gtagcgggca
    12061 gcagcgccaa cgggctgcat gctcaggccg gctgagccgc cggcccttgg tatcctgatc
    12121 gcagcctcgg ccgcaattcg gggcgtccgt ctgctcagac cgccttctcg tcgttcaact
    12181 tcttcgcaac gccgatccag tcgttgcgct cggcagcacc acgaagtccc atcgccttgt
    12241 cgagcgtggc gaggccggaa gcgtccagat cggcgatctg ccagaagtga tagacgccga
    12301 ggtcgttgag cttctgcgcc atcttcgggc cgacgcccgg gagcttgcgc aggtcgtccg
    12361 cctcgccgcg gggcgcatcg atccctttga acgcaccctt ggcttcgccc gcctcggcga
    12421 ccacaggcag ctcttcgacg ggcggtgtct cggatgcccc gaggtccacg ccgccgctgg
    12481 tctgctggcg ctcgaggccg tcgattgcgg ccttggcggc gaggtcgcag tagagcgtga
    12541 tggcacgacc ggcgtcgtcg ttgcccggca ccggaatgtc gatgccatcc gggtcgcagt
    12601 tggtatcgac gatggcgacg atcggaatgc cgagcttccg cgcctcggcg atggcgatct
    12661 gctccttgtt gacgtcgatc acgaacaaca gatccggcag gaagcccatg tccttgatgc
    12721 cgccgagcga ctgattgagc ttctcgcgct cgcgcgtcag ctgcagcagc tctttcttgg
    12781 tgcggccctg cgtttcgccc gccaggatct cgtccaactg gcggaggcgc cggatcgact
    12841 gggaaatcgt cttccagttg gtgagtgtgc cgccgagcca gcgatgattg atgtagtact
    12901 gcgccgcgcg cgacgcagaa tccttgatcg cgtccgaggc ctgccgcttg gtgccgacga
    12961 acagcacacg gccgcccttg gcgactgtgt cggagatctt cacgagcgcc tggtgcagca
    13021 gcggcaccgt ctgcgtgaga tcgacgatgt ggatgttgtt gcgtgcgccg aagatgaacg
    13081 gcgacatctt cgggttccag cggtgggact ggtggccgaa gtgaacgcca gcctcgagca
    13141 gctgacgcat attgaatgcg ggaagcgcca tagtgttcgg ttccttgacc ggttggagcg
    13201 tnnnnnnnnn nnnncgtgtg cctcttcgcc agcagtcaca tggccactga gctcaggacg
    13261 cactaaagag caccggtgtg gtagccggtt ggctgccacg agtccgcgtg cgagatggcg
    13321 cccttttatg ggccccgttc aggtttggca aggacgactt tccggtggca cttcggcgct
    13381 cttcgtccgc gccgacatgg atcggttaat ggcggacctg cgagaggctc agctcttgaa
    13441 ggcatggggc atgtcaccct ttccaagacc tacgtttagc aactcaaggc gtttctcgtg
    13501 caaggcactg aggcgtccgg cgccgcggtc gtagaggcga aacttcgcgc gattgacgac
    13561 atgatcgggc agttcctggt gcattatccg ggcgcaaagc cgcggcatcc cggcctcggc
    13621 ctcgtcgccc acccggtgac cggcacgccg ttcatcctgc tcgacgacta cgatgacgac
    13681 gagttgcgcc tccacttcat cgttcacgcc cacgcgaacc tcgacgatct cgaccccgcc
    13741 gccacagaat gttgacgcgg catgctccaa gaggccgcga gctgccgacg agagcacgtc
    13801 aggcgatcaa ccattgtgct gttgctggaa agaggcaggt ctgcgatatt gcggcgcaat
    13861 cgcaggccat atcggccaca agtgacgact tacggagacc cgccccatgg gcagcatgag
    13921 cgtatggcac tggctgatcg tcctcgccgt cgtcctgatc gcgttcggcg gcagcgggcg
    13981 catctcgtcc atcatgggcg acgtcgccaa gggcatcaag agcttcaaga agggcatgca
    14041 ggacgacgac gagccgaaga agcccgatcc ggcgaagccg aacgaccaga tcatggccgg
    14101 caagccgggc gaagctcccc gcgtcgagaa caaggtcggc tgatcgcggt cgcttccgcc
    14161 gcttcggtgg gcggacacgg atggggccgt cgcccgcggg atgatcgcaa ccggatttgc
    14221 cgcggcccat ctctccaggg cggccgagct tccggacgcg gttcacctgc cggaaatcga
    14281 gccgtgttga actgctgtcg cttcctgtcc ctcgggccgt gtccctcggg ctggttcggc
    14341 ggaacgtcag ggaaggaatg gaactgcgat gttcgagtac ggctcggtca agctcctgct
    14401 tcttgccatc gtcgcgctcg tcgtcgtcgg tcccaaggag ttccccgtcc tcctgcgcac
    14461 gatcggcaag tacgtcggca tgatccgcca gcaggcggcc gagttccgcc agcagtggga
    14521 cgacgccgtc cgcgacagcg agatcacgtc cctcaagaac gaggtcgaga aggtcggcca
    14581 ggatctgcag acgacgctcg acgagacgag gtcgcagatc gacgccgacc tgcacgnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnncccgcg gcgcaggccg ccgcggcagc cgtccgcgca
    14761 ggagcctgaa cccgtgcagg acgccacccc cacaaagctg ccgggcgacg agcccaagcg
    14821 cgacggccac gacgagatcg aggcctcgaa ggcaccgctg ctcgatcacc tgatcgagct
    14881 ccgcaagcgg ctcatctact cgctcatcgg catcggcatc gccttcgtcg tctgcttcta
    14941 ctttgcgaac cagatctacc aggtgctggt gcagccctat gtctccgtgc gcggagacaa
    15001 ggcgaagatg atcttcaccg caccccagga gttcttcttc acgaagctga agcttgcgct
    15061 cttcggcgcg gtctgcatcg ccttccccgt gatcgccaac cagatctaca agttcgtggc
    15121 gccgggcctc tacaagaacg agcgccaggc gttcctgccc tacctcgtga tgacaccgat
    15181 cctgttcatc gtcggcgcgc tggtggtgca caagatcgtc atgccgatga tgctgcagtt
    15241 cttcatcggt gacgagatca agacggcaaa cctcgagatc acgctcgagg cgcgcgtttc
    15301 ggagtacctc tcgctcatca tgacgctgat cctcggcttc ggcgtctgct tccagctccc
    15361 cgtggtcctc tcgctgctcg cccgcgcagg cctcatcgat gtggcgggtc tgaggagctt
    15421 ccgccgctac gccatcgtcg cgatcacggc cgtcgccgcg gtgctttcgc cgcccgaccc
    15481 cttttcgatg atcgccatga tgctgccgac cgtcctgctc tacgaggcgt ccatcctcat
    15541 gatcgagcac gacatcgtcg gcaagttcgg caacatcctc gacaagttcc gccgtaagcc
    15601 gccagcgccg cccgcgagca cgacgccgac gggctgaggc agaaggtagc cgcgcctggc
    15661 gcggccgtct ggagcgcgcc agccaacggc caagcagcct atttcacggc atcgctatcc
    15721 agtacgacga tccaagtgtc ggtgccgctc atcaggccat cgaattcgcc gcagccggca
    15781 ccggagacgc ccgccgccgc caagcgaccg tcgtcgagtg atactaacga gcggatgaac
    15841 ccgcgacctc ccaagatgcc tttcagctcg atcgattgct cactgcgccc gccggctcct
    15901 atgcggatca gcaacggccg tcgcgtatcc ggcgcgccct tgaaatccga cgcatcacat
    15961 ccgccgatcg ccaggcttgc atcgttgcca agccagacga gcgcccgagg cagcgcgagg
    16021 ccgccgcggc caaatcgccg ggaccagacc tgcttaccct cggcatcgac gacagcgacc
    16081 caacccggct cggctcgctt attcgaattg aaaaggcccg ccagagccac gccaccatcc
    16141 gcgaccagcc ccacagccga aacattcggc tgttcctccg tgtcggccgc gattctgagc
    16201 gtccacatct gtctcagttg gtcatcgacg agggccacga aaggtatgct gtcgccgaca
    16261 ccagcgtcgg cgtcgcgaat gctgtgatcc agagccacga aagcagtctt tcggtcaggg
    16321 cgcgacgcta cgagtacgcc cgagccgaga ttcatcccgg ggacgtgcca gcgggcgagc
    16381 gaacgcccat ccgtgccggt cacgtagatc gactgctcga gctgaccggt aatgacattg
    16441 atcgccgtgc cgtgcacgaa gctcgatggc cccaagaacg cgagtggggc acccgccctg
    16501 ccgccggccg ggacgtcact ctccgcaagc acctcgcccg cgagcgaaag ttccttgatc
    16561 catagtgttg agccacgcgt cgcgaggcca aaccccgcga cgacaatccg atcctggggt
    16621 gtcagcgaca gtccaccgac ctcgacgatc ccgccgtcct cgaaagtctt ttcccacttg
    16681 agcgcgccgc cgtcgtcgat gtgcaggatc gacagtttcg cgcctgtctc cttgataccg
    16741 cgccgcgcaa cgagcacccc gccgcccgga tggcgaacca tgtcctcgat ccaacctggg
    16801 ccgagtgcca cctcgaagcc tgcgggcctg gacgactgag cccgacagtc gccggacgcc
    16861 agcgtcatcg ccatcaccag agcgatcgtc accaggccgc acctgatcag gtttgggagc
    16921 cacgttccgc catcgcgacg atgcgcgaac cggtaccacg cgctcagtcg ttcgcagtcc
    16981 cggagtgtct cgatcatagc cgcgacacct ccacctcctt cgaggtgatg aactcgagca
    17041 gcgccggctg gcccttcttc gtcgccgcga tgccgcgctt gatcgccggc acgatgtcgg
    17101 ccggcttctc gatgcgctcg ccgtagccgc ccatggcgcg cgcgaaggcg gcgtagtcgc
    17161 ccgagatgtc cgtcgagcga tacttctctg tcgacaccgg catcaccttg agctcgatcg
    17221 ccatggagaa attgttcagc aggatcgaca ggatcgggat gcgctcgcgc accgccgtct
    17281 cgaagtccat gcccgtgaag ccgatcgcgg catcgcccca cacattgatg cagagcttgt
    17341 cgggcttggc gagcttcgcg cccatcgcga ggccaagacc gtagccgagt tgcgtgctct
    17401 tcccccagcc gagataggaa agcggcgtcg tcgcgcgcca gaacggcgag agctggtcgc
    17461 gcgggctccc cgcatcgtgc gtgatgatcg tgttgtcgat gtcgacggtc ttctgcaggt
    17521 cccagagcac gcggtaggga ttgagcgggg ccgcatcgtg cgtgagcttc ggcagccact
    17581 tggcgagcca gggctcgtaa gccgccttga tctcggcggc gacggccttt gcgtctttcg
    17641 gcgatttgcc gtgcagcttc tgcacctcct cgatcagggc ctgcagcacg atcttcgcgt
    17701 cacccacgac gccgagcgcg accggcacat ccttgttgat gtcggagggg tcgagcgtcg
    17761 cgtggatgat gcgcgcgccg gccggcatct tcacgccgaa gttcgtctcc gtgaacgagc
    17821 agccgatgcc gaggatcagg tccgacttgt cgaggaaggt gcgcacggtc tgggcaatcg
    17881 caagcccgcc tgagccgagc gacaacgggt gcgtctcggg gaaggcgctc tttccttcga
    17941 ggctcgtcgt cacgggtatg gcgagcagct cggcgagagt cttgagctcg gcccacgctt
    18001 ccgcccaatg cacgccttgc ccagcgtaga gcacggggcg cttcgcagtg accagcatct
    18061 tcgctgcctc acgcactgcc gcgagatcgg tgccggtgcg gatgtaggcg ggcgaagtgt
    18121 aaagcgaggg atcgcactcc tccgcgtagc tgtcggtcgg gatctcgacg agcacaggcc
    18181 cgccgcgacc agatttcagc ttctggaagg cgcggcggaa gatcgccggg atctccttcc
    18241 ccgccgtgat cggctccgcc gacttggtga tcgagcgcat cgagaccgaa gcgttgtagt
    18301 tgcggtcgac ccaagcgagg cggcgcgcgt agccctgcgc gaggacgagc agcggtacgc
    18361 tctcgctcca ggcctgcgcg acgccgccat aggcgttctc ggtgccaggt ccgtgctgca
    18421 tggcgaagac gccgatcttc cgccccgagg tcacgcgact gatcgcgtcc gccatgtgga
    18481 ggcccgtccg ctcctggcgc acgatgatcg ggcggatatc caccttcgcc gcgtgctcga
    18541 gcacgtggtt gaccggatag ccgatcaccc attccacgcc ctccttcttc aggatcgcgg
    18601 aaattgcatc ggcggttttc atggtcatgg gcgtctcccg gtatcgcgcg ctgcttgtcc
    18661 actcacgata agccggctcg atcgcaaaag tacatggcgg cggctctctg ccgcttgcac
    18721 ggctgcatct gcgagcatgc gtgctctggt cgggcttggc gatccggccg aaatctgctt
    18781 gttgcagcgc atcagcggca tgcgaggacg ccatgagcag cacgttgggc gcgagcggca
    18841 agacgaccgc tggcaacttc ttcgaggatt tccggctcgg ccaggtcttc gagcacgcaa
    18901 cgccgcgcac ggtgacgagc ggcgacgtcg ccctctatca ggcgctctac ggctcgcgct
    18961 ttgcgctgca gtcatcggac gagttcgcgc gcgggctcgg gttgccgcgc gcgccggtcg
    19021 acgatctcct cgtcttccac atcgtcttcg gcaagaccgt ccccgacatc tcgctcaatg
    19081 ccgtcgccaa cctcggctat gcggactgcc gctttatgga gtcggtctat ccgggcgaca
    19141 cgctcgcaac cacctcggag gtgatcggcc tcaaggagaa ctcgaaccac gagaccggcg
    19201 tcgtctacgt gcgttcgacg ggcacgaacc agcatggccg gccggtgctc atctattgcc
    19261 gctgggtgat ggtgaggaag cgcgacatgc tggcggcagt cgcagcggcg catgtgccgg
    19321 cgcttcccga gcgcgtcgat ccggcgaaac tcgccgcgac cttccccgcc tggagcacta
    19381 cggctgccgc ctgggcacgc tccggcacgg gcgcacgctt cgccgactac aaggccggag
    19441 agcgcatcga ccacgtcgac ggcatgacca tcgaggaggc cgagcaccag atggcgacgc
    19501 gcctctacca gaacacagcg aaggtgcact tcaaccagca cacggaaggc cagggccgct
    19561 tcaagcgccg gctcgtctac ggcggcgtcg tcatctcgct cgcccgcgcg ctctccttca
    19621 acggcctcgc gaacgccggc cgcatcgtcg cgataaacgg cggccgccac gtcgctccgg
    19681 cctttgccgg cgacaccgtg tatgcctgga gcgaggtcgt cgaaacggcg ccgcttccct
    19741 ctcgccgtga cgcgggtctc ctccgccttc gcaccttcgc aaccaagaac cgcccctgcg
    19801 ccgacttccc cggccgcgcc gagaagggcc acgccgacgg cgtgatcctc gagctcgact
    19861 actgggtggc cgttccgacc tgagcgtaac ggggtcttca ccgcgcctga catttgcgtg
    19921 atcaggcgcc ggtgccgccc aatcgttcca gcgctcggct agactttcgc tcccgaccgg
    19981 cctgcccaat cccgggttgc cgtgagctgc tcgtcgacaa gggagcccgc catgctcgag
    20041 ttgtcccgcc gcgatctgat cgtcagcggc gccgccgcga ccgccatgtt cggcctcaag
    20101 gggcccgtcg ccttcatcga tgccgcacat gcgcagcgcg ccgccgacag cttcaagtac
    20161 aaggtcggcg acatcgaggt gttcagcctg cacgagggcc aggtcgagcg cacgctggac
    20221 gccggcttcg tgcgcaacac cgccctcgac gacgtcaaga aggcgctcac tgcggcaagc
    20281 gtcggcccgg acaagttccc gaactatttc accgtcacgg gcgcccgcat cgacggcaag
    20341 ctcgtcctct tcgatgtcgg cttcggcggc aacggccagc tgaccgtcgg ccgcatggcc
    20401 gagaacatga aggccgccgg cctcgatccc accacggtct cgaccgtcgt catctcgcac
    20461 ttccacggtg atcacatctc aggcctgtgg accaaggaga cgaacgcgcc cgtcttcccg
    20521 aacgccgaga tcatgatgcc cgagaccgag tacaagcact ggacgaatcc ggccctcgtc
    20581 gagaaggtgc ccgaggcgca gcgcaatttc gtgcgccgca tccagtcaac cttcccgacc
    20641 tggaagaata tcaagctctt cgccgacaac gccgacgtgc tgccgggcgt gaaggcgatc
    20701 ccgacgcacg gccacacacc aggccacacg gctttcctcg tctcctcggg cagccagcag
    20761 ctcttcatcc agggcgacgt aagcggcatg catcagctgt tcgtgcgcaa tccgggcttc
    20821 cactcgatgt tcgatgccga tggaccgaag gccgaggcga cgcggcgcgc cttctacgat
    20881 cgcgtcatcg ccgagaaggg catgatcgcc ggctaccact tcggcttccc gaacgtcggc
    20941 acgctcgcca aggacggcaa cggctacgcc ttcaccgccg taaaggcgtg aggctggtcg
    21001 ctcagccaat actattgtga tggagcggca ccgattgggt gccgcaccat cgcgctggta
    21061 acttgttcaa cggtcgaggc tacgcgtctc gatcggtggc ttggccggcc tgcccgggtt
    21121 tgcgggcttc caagttggtg gacgcggctt caacggcgcg aaaatgggac gcgggccggg
    21181 cgccacctgc cccggccgcg atggcaccct cttcactcgc tcgcaattgc cggtcgagtc
    21241 gcagtaccac ttctcgccat ccttctcgca gatcgagaat tcgagcgtga ccttctcgca
    21301 cttgtagccg cctttgatca aatccgagat cggcttggcc tccgactgag cgatcgtgat
    21361 gccaaccagc acggccgcga tcgcaaactt actgaatgca aatttcatgt tcgatctcct
    21421 gacgtgatcc atgttcacat caagcgatgc tccgaacaga aaccattttg gatcgcggcc
    21481 ggtgtcatcg ggaagccggt cgaacggact ggccgggcat ggacgccaca atcgtcccgc
    21541 aagaccgctc ggcgccacgc gcgcgacgat cgctgcgcgg aaggtgggtc cgcgtgcttc
    21601 aggctgtcat gagacgccga gaattcacgc aggcaggcgt cggcggcagt cgggttcgcg
    21661 tgccgaaagc aacgtcgcaa agcgtgcgta gcgacggcgg ctcgcacaac acggattcgc
    21721 gctggcgcta ctgttacagc gccgccagcg cagccctgag cgccttggcg ttctcggcga
    21781 gcacctcgcg cttttccatc tggccggtgt ggggttgcag gcgctggccg tcccagcggg
    21841 gcagcacgtg gacgtggagg tggaatacca cctgcccgcc cgccgcttcg ttgaactgtt
    21901 ggatggtggt tccgtcggcg ctgtaagcta cgcgttgcgc gcgcgcgacc cgctgcaccg
    21961 tacgcatgac ggcggcgagc tgctcagggc tcgcatcaag gatcgatcgc accggcgcct
    22021 tcgggatcac gagcgcatgc ccgcgcgcgc ggggcatgat gtccatgaag gcgagcgtcg
    22081 cggcgtcttc gaaaacgcgc tcggccggca actcgccgcg caggatcttc gcgaacacat
    22141 tgctgtcgtc ataggccgtc atcacccgct ctccatcgat gccgtggcac cggatatacc
    22201 ccgtgcggct atgccgtcaa aagccggtgc cggccaggtg ctgccctcag cggacaccca
    22261 tccaaccgat gatcgcgcct tggatcagga gcacgccgag ccagtggagg ccatcgatca
    22321 cggtgagcgc gcgcctggag ccctggtagc catgattgac gacgagggtc gtcagcacga
    22381 agccgaacca gcagaaggcc gccgagatca tgccgttgcg caacgtcacc tggccctggc
    22441 ccaggtggcc gatgaggccg gccagcaccc aggccatgat cagctgcgcg atgatcgtta
    22501 agccgaacag cagcggcatg gacgtgcctg actttatgtc tgcttctgac tttccgaccg
    22561 ccgccatcca ctgctgcgaa aggatgccgt accacacgcc gccaaaaagg aagctcgcac
    22621 cggccgcgat caggatcgcc agataattta ttcctgcgaa tgccatcgct cggctccctc
    22681 gctcggttgc ttcaggccta tcgcgtgccc gggaccctca gcgtgaggac gcgtgcaccg
    22741 cgcttcacgg caatctgcca caacgccgga gccgtcgcga gccgcgtctc gacgtcggca
    22801 atcgacgcaa ccgcgctgcg tccgatatcg acgagaatat cgccgacacg aaacccatgc
    22861 cccgcggcga tggatgctgg cttcacggcc gtgacgacga cgccgacctc tgtctccagg
    22921 ctgagctcgt ccgcccttgc aggcgtaagg gtcgcgacgg ttgcaccgtc gagcggatgg
    22981 ggacccgcaa ggatgcggtg atcgtccttc gatggcttcg gcgcgcctct cagctgcaat
    23041 tccgtcgacc gcgagcgccc cttgcgcaga tagtcgagcc gcaccgtcgc gccgaccccg
    23101 accgtcgtca gccgatagag caggctcctc ggatcgagca cctcggtgcc gtcgatcgcc
    23161 gtgatcacat caccggcctg gagtccggcg gcggcagccg agctatcctg atgcacgcgc
    23221 ttcacatgcg cgccggcgat ccgtgcgagg ccgagcgact tcgcgagctc gctggtcacc
    23281 ggctccaggc gcgcaccgag ccaggggcgc tcgacgcggc ggcccgacat ggcgctctcg
    23341 atgtagacgc gcgcgaggtt cgacgggatc gcgaagccga tgccgtgcga gccgccggaa
    23401 cgcgaataga tcgacgtgtt gatgccgacg agccgcccgc tcatgtcgac gagcgctccg
    23461 cccgagttgc ccggattgat ggccgcgtcc gtctggatga agacctgcgc ctcggattgc
    23521 ccgacctccg tccgggcaat agccgacacg atcccgtgcg tcacggtctg gccgacgccg
    23581 aagggattgc cgatcgcaag cacgagatcg ccgacctcga tgccgtcgct gtcggcaagc
    23641 tccaggaagg cgaacgacgt cgccccgttg tcgatcttca ggatggcgat atcggtcttc
    23701 tcgtcggcga gcagcaaccg tgcctcgaac tcgcgccggt cggaggtgac gatgcggacc
    23761 tcgctcttcg ctcccttgat gacgtgatag ttcgtgacga gaaccccgtc gggcgagacg
    23821 aggacccctg acccgagcga gttctggacg cgctcgcgcg gcatgccgaa gcgctcggag
    23881 aagaagtggc gaaagattgg atcgtccatc gccggatggc gcacctcgac ccgagccgat
    23941 acgaacacgt tgacgacggc cggcgccgac ttgcgcacga tcggcgcaaa cgagaggagt
    24001 gccgcctccc ggctggccgg cgcgacccta ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgatgcc
    24121 tcatcctgcc ctcccgatca gacgcggcag cggcgcttca tctcgcccat cagctcgcgg
    24181 ccgacctcgc ccgcaaggat gacgccgcgt gagacttggc cgggcgcgag gccagccccg
    24241 cccggaccgc gaagctcggc cggcgcccgg tcggcctctc gcttggcgag cgccaactcc
    24301 tcgcaggtga gttcacgctt gcgctgttgc atggcgaaga ggcgaacacc gctcgcgtgc
    24361 gcggcgggcg tcgatggctc gcgcagccag cgcttgtcct tgtcagccat cagcgccttc
    24421 aacgcgaggg agcgctcatc cacgcagcgc ttgctgtcgt cgacacagct cgagcccgtc
    24481 ggcagcggcg ccatgaccgg ttcgctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnncagcgca gccgtgcgcc ggcgctcacc cggcagactg tctctcgtcc
    24601 cgcccattcc ctcacgcctc tctgctcccc gcatcccgca cttgcgagcg aggcgcttgt
    24661 ggttgcacca ttgagcccat agaagccagc gatggcgccg gtatggcatc ggcggctccg
    24721 cggcatcgag cgaggaagtt cccatggcac atgcagttcg cgtccataag tacggcggtc
    24781 ccgaagccct gacctacgac agcgtcgagg tcggcgcccc cgggcaaggc caagtcaaac
    24841 tcaagcagta tgccgtgggc ctgaactaca tcgatgtcta cctacgcacg ggcctctatc
    24901 caccccggca tggagggcgc cggcgaggtg ctggccgtgg gtcctggcgt gacagacctc
    24961 aaggtcggcg accgcgtcgc ctacgccggt cccccgggcg gctatgcctc cgagcgtctc
    25021 attccggccg accgggtcgt gaagatgccg gccaacatcg actacaaaac cgccgccgcc
    25081 atgatgctgc agggcatgac ggtgcgctac ctgctgcgca agacccacaa ggtcgggcca
    25141 ggtaccacgc tcctcttcca cgccgcggcc ggcggcgtcg gcctcatcgc ctgccagtgg
    25201 gcgaagcacc tcggcgcgac gctcatcggc actgccggct cggaggaaaa gtgcaagctc
    25261 gccaaggccg cgggtgctgc ccacgtgatc aactactcga aagacaactt cgtccagaag
    25321 gtgaaggaga tcacgggcgg caagggctgc gatgtcgtct acgattcgat cggcaaggac
    25381 accttccccg cgtcgctcga ctgcctgaag ccgctcggcc tcttcgtcac cttcggcaat
    25441 gcctcgggcc cgatcgaaaa catgagcccg ggcatcctcg gcgccaaggg ctcgctgttc
    25501 atgacgcgcc cctcgctaat gacctacacg tcgagccgcg ccgatctcgt cgagacggcc
    25561 aacgatctct tcgacgtcgt cggcaagggc gccgtcaaga tcaacgtcaa ccacacctat
    25621 gccctgaagg acgcagcgca ggctcatcgc gacctcgaag gccgcaagac cacgggctct
    25681 gtggtgctga tcccctgagg ccaacacccg ggcccaatat cctaagcctt ccaagacacc
    25741 aaatggacgg ttctggctct gctcccttcc cccttgatgg gggaagggat tgaggttgtg
    25801 cgtgagggtc gaggatcagc ccggtcggat agctgctcgt gacctgctcc ccgtcttccc
    25861 tccaatcaaa acattatgct gttctgcctt tcttctcgag ggcccattct tatgttcggc
    25921 cccctgcgga gccattcttc cgtcccggcc gcattctcga cacgccgtcg cgcgcagggg
    25981 tggtcaaggt cggccggcac accgacggtg acgctggctc ggtgcttcca ggccgccctt
    26041 gacggccccg agcacgacgg caggactgac gcgtccgggc gaggcttgct ctgaccaggc
    26101 gtgagcgaac tcctcgggcg tctggtagcc gaggctagaa tgcgggcata cctcgttgta
    26161 gtcgatgcgc caggcctcga tgatccggcg cgcctccccg agcgaaccga acaggtgctc
    26221 gttgaggcac tcgtcgcgga gcttgccgtt gaagctctcc atgatgccgt tctgcatcgg
    26281 ctgacccggc tggatgtaat gccattcgac cccagtctcc gtcgtccaat tcagcatcgt
    26341 attgcaggtc agctcggtgc cattgtcgga cacgatcatc gccggcttgc cccacatcgc
    26401 caccggccgg tcgagcacgc gcacgatccg cttaccccgg cagcgacgtg tcaacctcga
    26461 cgccgaggcc gcgccgcgtg aactggtcct cgacgttgaa gacgcggaac ttgcgccccg
    26521 tttcgagcgc atccgacacg aagtcgagca cccagatcgc gttgggatgc agcgctttct
    26581 ccagcggtgc ccgcgtgccg atcgcccgct tcctgccatc gagcttaccg aggatttgca
    26641 gagctcttgt cacttgatca ctgccccgga ggccgtggcg gtcgtatcgg ccggtcgcgg
    26701 ccgcagctct agtgcgcctc gcgccaggaa ggacaccgcc cggcgggccg tgtatcactc
    26761 tcggaagaga cgcatgggtg aaggtaaggc agatgagcag gatcgagcgc attccgcatg
    26821 gcagccactg gggtgcctac tggctgctcg aggcgggacg catcgtcggc gtagagccgt
    26881 tcgagggcga ccccgatccc tcgccgatca tcaagtcggt cgccgcctgg gccgaccctc
    26941 atcgccgcgt catgcagccg atggcgcgca agtcctggct tgctgcacga cgcgccgggc
    27001 ggctcgcgac ggtcgatgag cgcgcgcggc gcggccgcga tacctttaca ccgatctcct
    27061 gggatgaggc gctgagcctc acagcgaacg aaattcgccg tgtgattgcc gaccatggca
    27121 accgctcgat ctttgccggc tcctacggct ggacgagcag cgggcgcttc catcacgcct
    27181 cgacgctgct ccgccgcacg ctcaatctgg tcggcggctt tacgggacac gccgatacgt
    27241 actcgatcgc ggcggggcct gtgatcctgc gccacacgct cggcaacacg gatgcctgcc
    27301 agggccgcgc caacaccctc gagaatgtcg cgaagaacgc cgagacgctg gtcgtgttcg
    27361 gggcgatggc gacgcggacg gcccagagcg aggctggcgg catcgccaag caccggctcg
    27421 ccgaaaatct ccgggccatt gccgagcgcg gcgtccgcgt gatcctcgtc tcgccgcagc
    27481 gcgacgatct gccggactgg ctcggcgccg agtggtggcc gatcaagccg aacacggata
    27541 ccgcgctgat gctggccctc gcgcaagaga tcgtcgggac gggccggcac gatgccgaat
    27601 tcctcgcgcg ccactgctcg gggtcggaac tctatctcga ctatctgagc ggcaccacgg
    27661 acggcacgcc gaagacggcc gaatgggcag ccgccatcac cggactcgag cctgcgcaga
    27721 tccgtgccct tgccgacacg cttgtccgca cgcgcagcat gctctcgttg agctggagtc
    27781 tgcagcgcgc gagccacggc gagcagccct tctggtcggc actcggcctt gccgctgtcg
    27841 ccggccagat cggcaagcca gggggcggcg tcgcctacgg ctacggctcg ctcggtggcg
    27901 tcggcggacc tgtgacgctt tcgaagacgc ccgccatgtc acagctctcg aacgacctcg
    27961 acagcttcat ccccgtcgcc cgcgtcaccg acatgctgga aagtccgggc aaggcctatg
    28021 actacgaagg gaagacgcgg cactatcccg atatccgcat ggtctactgg gccggcggca
    28081 acccctatca tcaccaccag gacctcaatc gtctcgagcg cgcctgggca ctccccgaga
    28141 cgatcgtcgt ccaggatccg atgtggacgc cgacggcgct ccgggcggac atcgtgctgc
    28201 cggcgacgac ctcgatcgag cggaacgacc tcgcgggcaa ccgccgctcg gacacgatcg
    28261 tcgccatgcg gaaggcgatc gatcccatcg gcgaggcgcg ctccgatttc gacatcttct
    28321 caggccttgc cgcgaagctc ggcgttcgcg agcgcttcac ggaaggccgc gacgagatgg
    28381 cctgggtgcg ccacctctac gaggcgacgc ggcaggacgc caaggagcgc tgcaacttcg
    28441 acatgccgga cttcgatacg ttctgggagg ccggcacagc ggcgatcccg acaatcacgg
    28501 atcagacgca tctcgccgcg taccgcgccg acccagagaa gcatgtgctt gccactgaaa
    28561 gcggacgcat cgtgctcggc agcgcgctgc tcacggcgaa gaactacgcg gactgccgcc
    28621 cccatcccgc ctggctgccg ccagccgagt ggctcgatcc ggcggcggag aaggccggtg
    28681 ccttccacat gctgacgccg cagcccgacg gccgcctgca cagccagctc gtccacgcgg
    28741 gcccgagcca tgcccgcatg cccgacggcc gcgagcggct gcgcatcaat cccgtggatg
    28801 ccaagcgcct cgatctcaag aagggcgacg tcgcacgcgt gtggaacggt cgcggggcct
    28861 gccttgccgt cgccgagccc tacgacggca ttcgccaggg tgtcgtcgcc ttgccgaccg
    28921 gggcgtggct gacgcccggc ggagatgcag gcgcgcccga gcttgccggc aacccgaacg
    28981 tgctgacgcc cgacatcccg acctcgagcc tcggccaggg ctgcgcggcg caaacctgcc
    29041 tcgtgcacgt cgagcgctgg cgcggcaatg acagcgtggc cgccgnnnnn nnnnnnnnac
    29101 gacgaggcgc tgacgcgggc ggtcgcgggt gaacgcgttt aggtgtgcct tgtggcggcg
    29161 gagcgttcgt tttctcaatt gatcaatgga cgcgccttag ctttctacgg tctaggctct
    29221 gatctcgttg gtcgggatta ttgccaaaga ggccacgaat gatttggctg atgcgacaag
    29281 cgtccatcgc ggctgcagtc ctcacaatcg ccaccctcgg catcgggaag ttgccggttc
    29341 gcgcagatcc acgctgcgac gagccatgtc agctcgaccg aggcgaatgt atcgtttgtg
    29401 gaaatcggct ggtctgctac tcggtcgatc gctgcgagcg atcatcagtg acgtactatc
    29461 agctgccgaa gaacttcttc gacaagccgg atttcatcca gaagaagctc ctcgacaagc
    29521 tgatccgccg acacgtgcga accttgcgcc aaccactttg acatgattgg ctcaagatgc
    29581 cctcaatcaa ctggtccctc tatctcggcc atttccagac ggcgctcctc tatgggctag
    29641 ttccgattgc tgtgatggtc gtgctgctac acggcctcct ttgggcctcg actttccggc
    29701 ggaccgacga gcatcgcgcg atcctgcagt tccactgcct gccattcacg gcgctcggcg
    29761 tgctcaccgg catgttcaca ggcgccagcc ggacagcggt agtcggtgtg atcgtgccgc
    29821 cggtgctcac cttcatatcg gggctcgccg cctatgttct tggcaaggat gcgaagccca
    29881 tctggcgccg aattgtgccg ctcgcaatcg ccatgatgac gttcggcacg atgttcggcg
    29941 ctgccttcgg tgcgtccatg cgccaagcca acgaggagcg tgatcgccag ctagagcgtg
    30001 agaagcttcg ctacgagaag gttgagcttc cgttcatgca gaaggcggtc gagaaaggcg
    30061 gttcaacgcc ggcaccggcc tcagccaatc ccggcgccgt gcctagcgtt tccatcaaat
    30121 aggcggcggt caactatttg ccgacactgt tcgccgatct aacgctcacc gtcgcccgcc
    30181 ggcaagacgt cgggcctcgg ggcgcgaccg caatcgcagc atcgcgacga caccgaggaa
    30241 cgggccgatc gccagcgtcg cgaaggccca gcgccagctg agccatgaga cggcgtaggg
    30301 catcatgtga atggtgacga gggtgagcag gaatccgctc gcggtctgca gcgtcagcac
    30361 ggagccgacg acagccggct cggtcagctc ggcgacggcg gttgagaact gggcagaatc
    30421 ggcgacgatg gcgacacccc acaccatgag caacatcagc agtaggacga gcggcccgtc
    30481 gaacaggagc ccaacgacga gacagcatgc gcctgagacg cccatggcaa tcgaggtgac
    30541 ggcggtacgg ccgatgcgat ccgcgagcca gccggcaccg aagcagccga tcgcaccgat
    30601 gccgatcacg gcgaaggtgg cgagcgccgc gctgcgctgt ggattggccg gagccaagcc
    30661 cgcagcgaag ctcgccgcca ggaacgcgcc gatccacgcc cacatagcgt agagctccca
    30721 catatggccg aggtagccga ggatcgcgag gcgcgtcggt cgatccgcaa gtgcctggcc
    30781 gacgagatcg cggcggaagg cgccgccttt cgagaatctc ggcccgacct ccgcggcgag
    30841 aatgagccct gccccgaggg tcgctgacag cgaggctgcg atcatcatcg cctgccagct
    30901 gtgaaacccg aatgcgttga agagatgtgg gctcgccgag ccgagcgtca gcgcgccgac
    30961 gagcagcccg accagaaatc ccatgtcctg gcgaccggtc gcccccgccc acgatgcggc
    31021 aagcttcatg ccgacggggt agatgcctgc gagcgtcgcc ccggtgatga agcgaagcag
    31081 gatcaccatc ggcgagctcg gatcgagcat cgcgacgagg ccgttggcga tggccccgac
    31141 gagcgcgctc gccgcgaaga agcggcgcgg ctcgagccgg tcggcaaggc cgagcatcgc
    31201 gctcgcgacg ctgccggcgc cgaagccgag ctggaccgcg ctcgtgaaca gcgactgctg
    31261 gagggcagaa atggcgcccg tcgcacgcag cgatggcacc acggccgtcg ccgagaacca
    31321 gagcgcgagg gtgagcacct cggcgaccgc gatgatggcg atagcgcgcg accgtcccgt
    31381 gcttttcatc acgccgcggg agcgaacggc tgaaccttca tgccagcgcc ctccagtacg
    31441 tcgcgcacgc ggcgcgcctg cgccaccgcc gacggcttgt cgccatgcac gcagatcgtc
    31501 tcgatgcgag ccttgatgcg tttgccgctg cgagcgacga tcacctggtc acgcgccatc
    31561 tgcagcgcac gctcagcggc gatgaccggg tcgtcgatca cggagccggc aatcttgcgc
    31621 ggcgtgagct ggccgtcgtc ctcgtaggtg cggtcggcga aggcttccat gacggccttc
    31681 aggccgagct tctcggcgat gcgttccgtt ggcaggcccg gcatcgccac gtagacgagg
    31741 ctccggtcga cgcccttgat ggcgcggccg accgcctcgg cgaactcagg ttcggcgttg
    31801 gtaagattgc cgagcgcgcc gtgtgccttc acgaacgtca ccttgtgacc ggcgagagag
    31861 gccatgccct gcatggcgcc gatctggtag gcgaccatgc gctcgatctc ggccatgctg
    31921 tcgccgcgga tcacgcgacg gccgaagccc cacagatcgt tgaagctcgg gtgcgcgccg
    31981 agcgcgatgc ccttcgcctt cgccttctcg gcgatgcgga acatgatgtt cgggtcgcct
    32041 gcatgccagc cgcaggcgac gttggcggac gagatgatct cgagcatcgc gtcgtcgtcg
    32101 cccatctgcc agggaccgaa gccctcgccc atgtccgcat tgaggtcgat acgcatgctc
    32161 gtctccaccg ttcgctgccg ggcgcagcgg cccgcgtgtc tcaatgggat gatgcagcgc
    32221 ggggaggcct gagacaagtg ccgccaaggc tcagcgctcg ctgcgacggc tcacgtagcc
    32281 gttctcgatg tcgctcgcga tcgcgaccgg atcgcgctcg ccgggcgggc cgtagccgcc
    32341 gccaccggca gtgacgaggc tgaacacgtc acccttgccg atccgcatgc ctgcaagctt
    32401 cgaggggagt tgctccgtcc gcccgtcggc acgcgtcaac gtagcgaagc cggcagcgcc
    32461 gggtgcgccg ccctcgatgc catagggccg cgtcttgaag cgatcgaggt tgagcgagag
    32521 cgtgcctgcg ggggcatcga tgcggaacga gcgctcgagg ccgcagccgc cgcgatgccg
    32581 gccgggccca cccgaatccg gcgcgaggct gtagcgctgg aaggtgatcg gataggtgtt
    32641 ctcgagcatc tcgacgggcg cgttggcgat gttgtggagg ccgcacgaga agccgttggc
    32701 gccgtcgccc tccgggtgtc cgccccagcc gccacaggtg aggtcgaagt agacctggcg
    32761 gcgtccatcg gcatggaaga gattggtcgc gaaggcgtag ctgacgccgt agtaggcggc
    32821 gggcaccttg cccggcaccg cgcgcgagaa ggcgcccatg acggtattgc agacgcgatg
    32881 gccgacagcc atgcggttgg cgacgggggc gggtgagcgc gcgttcacgc agcttccctc
    32941 gggcgcgacg atggtcaccg gctcatagac gcccgcgttc gccggaatgt cgctgccgat
    33001 cgacatgagg agcccgcaga aggcccccga agcagacatg ttgcgcgtgc agttgacggg
    33061 cccctgcgct tgcgggctcg agcccgtcat gtcgatcgtg agcgcgtcac ccttgatgcg
    33121 cagcgcgacg gcgatcctca ggcgcgtctc gcggtcgatg ccgtcgtcgt cgacgaaatc
    33181 ctcaaacgaa tacgtgccgt cgggaatggc ggcgatcgca cgccgcatcg ccatctctga
    33241 ctgccggcga atgccgcgac acgcgtcctg cagccggtcc gcgccgtagc gcttggcaat
    33301 tcgatgcagg cccttcacgc cgatgtcgag ggctgctagc tgcgaggcga aatcgccgcc
    33361 gacattggcg ggctcgcgcg aattgcgcaa gaggagtgcg atcacctcgg tgttgcgccg
    33421 ccccgcgttg tcgagccgca tcggcgggat acggaagccc tcctggtaga tttcggtcgc
    33481 ggctgcgtag tagctccctg gcgcgccacc gccgacatcg agatggtgga cgagcacgcc
    33541 ggcgatgccg acgagacgtc catcgacgaa ggccggcttg aacgtcagga tgtcgttcaa
    33601 atgctggccg ccggcgtagg ggtcgttggt gatgatgatg tcgccctcgc gccaatcctt
    33661 gagcgggatg tgccgcgcga ggatgtcgtc gaggcagctc gacatggagt tcaagtgtac
    33721 gggaatgcag gtcgcctgcg cgacgctctt gccgtcggca tcgaacatgc acgtcgaata
    33781 gtcgaggaga tcgcgcacca cgctcgaccg cgacgtgcgc cagattgtga cgcccatctc
    33841 ctcggccgcc gcgacgagcg cgttgcgaat gacctcgagg ctcacggcat cgagcggctt
    33901 catgtgcggt ctccgctgtc cttggtggcg acgagatcgc cggtcggcgc gacgacgagc
    33961 gaccagccgg gcggcacgag gtgcgtcgcg tgctcctcct cgacaatcag cggtccggca
    34021 ttgcgtgagt ctgaggcgag gcgcgcacgg tcgagcacag ggcaatcgtg ccagccttcg
    34081 ccgacgagca cgcgccgtcg ccctttcgcg ggatcgccct tcgcaatcgg gggcgtgtgc
    34141 tcgatcggct tggcgaggcg cccggtcgcc gcgatccgga ccgtgacgat gtccgggatc
    34201 acatgtggct cggcgtgcgc gtattgtgcc aggtgccgct cgtggaagcg cgccacggcc
    34261 tcggcgagcc ttgcaggtgt ctcgagtggg acctggattt cgtaggcctg gcccggatag
    34321 cgcatgtcga ggaagacagg gagctgctgc gcctccgccg cgacgccgtc acgggtgagc
    34381 atttggcgcc cggcggcgtc gagctcggcg acgatcgctg agagctgcgg cagcgatccg
    34441 gcatcagcct tcatgaggcg cgcgcgagcg agcgcgtgca cgatgtcgga atagagcatg
    34501 ccccaggcgc tgagcgtgcc cggatcgcgg gggaagacca cgcgggtgat gccgatctcg
    34561 tccgccgtct cgcaggcatg gagcccgctc gcgccnnnnn nnnnnnnnnn nnnnnnnnnn
    34621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcccgc catgcgcgag gtggcgacgc
    34681 gggcgatgcc gagggccgca tcgtcgaggc tgagcgagag cggggttgcg accttggtgc
    34741 gcaccgcagc cgtcgcgccc tccgcatcga gcgacatggc gccaccgagg aaatagccgg
    34801 ggtcgatccg gcccatgacg acgttggcgt cggtgaccgt cggctcggtg cccccgcggc
    34861 ggtatgagac gggcccgggc ttggcgccgg cgctctcggg accgacgcgc atgcgcccgc
    34921 ctgcctccgc gcgggcgatc gagccgccgc ccgagccgat ggtgcggatc tcgatcatgg
    34981 ggaggcgcac cggcaggccg tcgacgacgc cttccgtgac gaggcggatc gcaccctcgt
    35041 tgacgatcga gacgtcatag ctcgtcccgc ccatgtcgac ggcgaccatg ttcggcatgg
    35101 cgagacgccg cgcgatggtc tctccggcga gcgccccgcc gctcgggccg gagagcagga
    35161 ggcgcgcggg ctgcaccgcc gcggtctcgg gcgtcgtcac accgccgttc gactgcacga
    35221 ggtagacggg cgccttgaag cccgcagccg cgagcctcgt gcgcagcttc tcgagatagc
    35281 cgcgcacgac cggcatcagc agcgcgttga ggactgtcgt cgaggcgcgc tcgaactcgc
    35341 ggatctcggg gctcacctcg tgcgagagcg acacgtcgag tccaggtgcc aagcgcgcaa
    35401 gcagctcgcc gacccgcctt tcgtgcgccg gattggcgta ggcgtggagg agcgccacag
    35461 cgactgcgcg cacacctgag gccttcaagc gctcggcaat tgccgccaca tccgcctcat
    35521 cgagcggcgt ctcgaccccg cccgtggcac gcatgcgctc tcttacggtg agtcgcaagg
    35581 cgcgcggaat caggatcgcg cgcggctcgg ccttcgaggc atagacgtcg cggcggaagt
    35641 ggcggccgat ctcgaggaca tcctcgaaac cgtgtgtcgt gatgagcgcg cctttcggca
    35701 ggcgccgctg caacacggcg ttggtcgcga tcgtggtgcc gtgcatcagc gcggagacgt
    35761 cggcgagcga gaagccgatg cgctcgctgg caagcttgag gccggcgatc aagccttcgg
    35821 aggggtcatc tggcgtggtc ggcgtcttgg cggcatgggt ttgcccggtc gcctcttcga
    35881 ggacctggag atcagtgaac gtaccgccga tatcgacggc gacgcggaag gagcggcgct
    35941 ttgctttggg cttcatcggg gttccggcgg acacaggcca tcaagggacg gcggcgacag
    36001 tggagtgcgg cgccgctgcg agcaacggcg aagttgccgc gagaccgtgc attgccgcag
    36061 gcatgccatg ccagagaccg acaactccgc ccttcccgag cagcatcggc ttcgcctacg
    36121 tttgcacacc ccgggccaga tcaagcgagc agatcatgcc gaaagcagcg acatcccccg
    36181 ccgagcagcg cgtcctcgac tggctcgccc gccacgagcg cgagatgatc ggcctcatcg
    36241 aggaggtcgt gaacatcgac agcggcagct acgacaaggc gggtgtcgac agggtcggcg
    36301 agcgcttcat ccgcttcttc gaggggcgcg gcatgattgc ccggcgcgag gcacacgcct
    36361 cgtttggcga cgccattcac gtccgcctcg acgacacgcc gaccaacgag cggcccgtcg
    36421 tgctgatggg ccatcgcgac accgtgttcc cgaaaggcga ggcgggtcgt cgcccattcc
    36481 gcatcgaggg cgggcgcgcc tatggccctg gcgtctcgga tatgaaagcc gggctcgtca
    36541 tgaacgcctt cgtattggcg gccttcaagg agctcggcgg cgcgccggcc ccgctcgccg
    36601 gcctcatcac cagcgacgag gagatcgcct cgccctcctc gcggcccgtg atcgagcgcg
    36661 tcgcgcgcga agctcgttgc gtcttcaatt ccgagcccgg ccggccgacg ggcgcggtcg
    36721 tgaccggtag aaaaggcggc gtcttcatgc ggatggtgat cgaggggcgc gcggcgcatt
    36781 ccggcggcaa cttcgagtac ggcatcagcg cgatcggcga gctcgcgcac aagatcgtcg
    36841 cgatccatgc gctgactgac ctgaagcgcg gcgtcacctt gaacgtgggc ctcgtcaagg
    36901 gcggccagtc ggtgaatacg acggcacctg aggccgaggc gcagatcgat ttccgctacg
    36961 tgacgccgaa ggaccgcgcc gagatcctcg cccgcctcga ggcgatcgtc gccgcgagca
    37021 ctgtccccgg gaccaaggcg acactcacga tcagcggcga attcctgccg ctcaatgcga
    37081 cgccctcctc ggaggagctt ttcggcggct acaaggcagc ctccgccgat ctcgggctga
    37141 cggtcgcagg cgagttcaca gggggctgcg cggattcagg ctacacggcc ggcgtcggct
    37201 gcccgacgct ctgcgccgtc ggccccgtcg gcggcaaggc gcacacggcg gaagagtacc
    37261 tcgaggtcgc ctcgatcgtg ccccgcgccc aggccctcgc ccttgcgatc ctgcgcctgt
    37321 cgcccgacaa gcgctgagag ggaaatccga tcaggcatga tccatgaacg atgcgacgag
    37381 cgttgccgcc gaggaccagt acgtcacgca cgccgagttg gccccggaag aaggccagcc
    37441 caacgcgctc gacaaccatc gtctgcacga gctgatcctg cgcctgcaga gcgagaacag
    37501 gcacctcgcg gcgatcaccg tccgcggttg gtcgatcacc aatcccatcg agctcgggct
    37561 ccacgggacg cgcggtctgc cgctgccgcg actgtcgttc gtctcgtgca gcaatctcaa
    37621 actttcggca cgctttgcga ggttgaacgc cctcagcctc aacgcctgcg cgatcgagca
    37681 actggatctc acggatgcgg ttatcgatgg gccgctcgac ctcgaacaca gcaccatcac
    37741 aggcgagcac gatacgacgc tcgctctgac ggggatcaag gcccaatcga tcttgatcac
    37801 ccggctcagt cggagcgacg agaatttcta cctctcgatc gatcttggcg cagcacggat
    37861 cgagcgcggc ctcgtcatcg acgccgtccg gctcaaagga ccgctgatgc tcgatcgcac
    37921 gtctttgggc gcggacgcga cgatcacggg cagccgtttc gagcgtcgca tgggagcggc
    37981 ggtctccgcg cgcgcgctcg tgactggcgg ccccctctcg ttctccagct gccaagccga
    38041 cggcgccgtc aatctcgccg atgcccggat cggcggggac ctcagcttcg atcacgtcga
    38101 gatccgctcg acgccgagtg aatcggtgct gctcgccgat accacggtgc aaggcactgt
    38161 ccgggtggcg aactcgacct gcaccggcac gatacagctg agaatgtgcg cgatctcggg
    38221 ctcggtcgcc gtcgagcaca cgacctgcaa cccgctgctc ggcgccgcga tcagcatact
    38281 cggcggcaag atcggcgcag aaatcacctt ggtcggattg aagctgtcgg cgcagctcga
    38341 ggtccgctcg gtcggtatcg gcctcaacat ggtgctcgac gacgttgcaa ttaccggcga
    38401 cgagagattt ccgatcaatc ttctgctcaa tcggatcggt ggcggtctcg acatcatcaa
    38461 gctccgctac gccggctatg gcacactgcg cgcctgctcg atcggggcga gcctctattt
    38521 ggaggagatc acgctcaacc cgcggcgcat cgaggatcag cgctcgctct acgattgcaa
    38581 cctgctcgcc acggagaacg tggtcggcgg caagctgaca ctcggcacga tcgcaagcga
    38641 gggcgaggac gcgcgcggcc tcgtcgatct gcgcaacacg cgctgtgcgt cgttcggctt
    38701 cctgggccac gccgattccg agaacattca catcaatctg aacgggctcg actacggcaa
    38761 gatagatcag ctaaatctcg cgagcgcgaa gttcctgcgg gcgatggtcg accgcaacga
    38821 gcggcgccgg tacgagttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc cgcagcctca
    38941 ggcctacatg catctcgccc gcgtgctgac caacgaaggc ctcgacgagg acgccgagaa
    39001 ggtgatggtt tccatgcgcc gctcgacccg gttcgcgggc gcgatgcgta agccgctgac
    39061 ctacctcatc tccggccttt tcgatgtctg cttcggctat ggctacagcc gctggaaggc
    39121 actcataacc cttacgctct ggatttgcct cggctggtgg ggtgtcacgc aggcgctggc
    39181 gcagggcgtc atggtggtcg acgttcaggc cgtgacgacg ggcggcggcc cgctcgcgcg
    39241 ctgggcgacc gtgtcgagcc ctcccgagcc gccgtgcaag ggtcagatcg agccgctctt
    39301 ctatgccatt gacctcacca tcccggtcct cgacctcggt caggtgcgca aatgcacgat
    39361 ccgtctcgac gaggccgagg accatctctc cgtctggcag aaatcgacga cgcgctggtg
    39421 gatgctgcgc tacgtctacg agctgctcgg cgggctcatc gtctcgctca ccgcgctcac
    39481 cttcacgggc accctgaaaa ccaagctctg acagttcctc actcgtcgcc gggccggctt
    39541 tgacgcatcg acttcaccgt gctgcggcgc gctttgcctt cgaggcggcg ctccttcgag
    39601 gctttggtgg gacgcgtctt gacgcgcggc ttcggcggcg tcgcggcgac ctcgatcagc
    39661 tcgcgtagcc gctctcgggc atccgagcgg ttcatctcct gcgtgcgatg ccggtcggcg
    39721 cggatgacga gcacgccgtc cttggtgaga cggcgcccgg caagccgctc gagccgaacg
    39781 cgtacgggct ccggcaggct ccgggaggcg cgcacgtcga atctgagctc gacggcggac
    39841 gccaccttgt tgacgttctg cccgccgggt cccgaggcgc gcatgtactg ctcggagagc
    39901 tcgctctcgt cgatcgatag tgtggcggtg attctcatcg gttcacctta acttccgcga
    39961 cgaccttaat cgagccgaca aaacagccat taccgttttg ccacggacag ggttgcggac
    40021 gaggaacaag ctactgtaag catttggcaa atggcctgac ggaatttgcc gcattcggtg
    40081 ctgcgatgcc aatcgaggtc tgcagaatgc tgcagcgtcg ccttggcgct ttcgcgagtt
    40141 cggcctgtgg tgtagtcatt gatgatgttg ctgcgaccaa gacacgaaat cgcgcgggcc
    40201 agatccgcgg gcgcggcaaa gggaggagtt gagagtgcaa gaccgtacgc ccgcaagcca
    40261 ggctgtggca cagccttcgg cggcagcgct tgccgttgca catgacgacg gccccacgac
    40321 gagggcgaca gcgcgtgacg cagctgcaag tcctgcggcc gtgccgcgtc acggcgcacc
    40381 ggtcctgtcg atcaaggact tgcacgttca cttcgtgacg acccatggca ctgttcgcgc
    40441 cgtcgagggc gtcagctacg acgttcacgc cggcgagatg gtggcgattg tcggggagtc
    40501 cggctccggc aagtcggtct cggcgttgac cgtgatgcgg ctgctgccgc caaacacggc
    40561 acgcatcatg aatggatcga tctcgttcga gggccgcgaa ctcctgaagc tcagcgacga
    40621 ggagatgcgc cagattcgtg gccgctccgt ttcgatgata ttccaggagc cgatgacctc
    40681 gctgaacccg gtgctcacga tcgggctgca gatcaaggag ccgctgctta tccaccttgg
    40741 catgagcgag gagcaggcaa cgactcgggc gatcgagctt ctgaccctcg tcggcatcac
    40801 cgatccctcg agccggctcg cccaatatcc ccatcagctt tcgggcggca tg
//
LOCUS       DAORNH010000009        37385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_836_length_37385_cov_2.632253, whole genome shotgun sequence.
ACCESSION   DAORNH010000009 DAORNH010000000
VERSION     DAORNH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 37385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37385
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_37385_cov_2.632253"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PK264_01790"
     CDS             complement(<1..479)
                     /locus_tag="PK264_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRD74660.1"
                     /translation="MSKAPLTPSKAPAAIAAHLQRITHQHFFRTFEDWLALAVNAFLR
                     DDDAYMAIVRRYGPRTAPMGTTDHPADHFARALGTWMAAMQAEPADYLGRVYEEQAVA
                     NAYAGQFFTPEPVVELMAAITMPEIPDAAIVADPACGSVRFIVPVHAPLISFGSPSS"
     gene            complement(476..808)
                     /locus_tag="PK264_01795"
     CDS             complement(476..808)
                     /locus_tag="PK264_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74661.1"
                     /translation="MSSRPVRTHWFTWRHLRCKVVEKPCDLGDHDTLLELHVIAARDT
                     PVPITTTGFLAHFLSTAALTGAGGPTTFFTTWMDREARSRDYQLAEFLWRQGDLFDRL
                     VPDEGRDA"
     gene            complement(805..1143)
                     /locus_tag="PK264_01800"
     CDS             complement(805..1143)
                     /locus_tag="PK264_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74662.1"
                     /translation="MPTTPATPTAFASIQCRIIDTVHDEPVEIAEGLFHEMLNALPPH
                     DWRNTAAGESFKAIEPTAGPISAIYVRIADRHFRLVGRADLGHDAIIARVGASEAFRN
                     PRQHDSGPMR"
     gene            1552..1797
                     /locus_tag="PK264_01805"
     CDS             1552..1797
                     /locus_tag="PK264_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74663.1"
                     /translation="MPRFQRNTDDLGVTEEHYRMFRSLVPDIIGDMDQKRAAAIEAAK
                     IYERALKRGSRAAAEQAARDWLAELQEQVGAQHGMGR"
     gene            complement(1856..2413)
                     /locus_tag="PK264_01810"
     CDS             complement(1856..2413)
                     /locus_tag="PK264_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74664.1"
                     /translation="MNPSETGNFIVGQNRAKQPGTNTPDFTGRIGIPGREHEFPVALW
                     AGRDRHGKLYFSGRITPTPITDNVMAQLESLTDRPVHDATDMADAGPGLTLRPFQMVA
                     FANKSKEPVDTDSKDEAATRAKRPDFWARVNPGDGSPVFQVGIWAGQDRYGRPILRGE
                     TSYPQPAKAAEKAPARRKADARDSR"
     gene            complement(2969..3265)
                     /locus_tag="PK264_01815"
     CDS             complement(2969..3265)
                     /locus_tag="PK264_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74665.1"
                     /translation="MPHLAYTDHGWQRKNERCKGDLLVDLLLEHFDRDVYVGSGDYAW
                     SISRRRCMELRRAGVISASQADGLAKLILVMTEDERVKTLIKGGKALGRYFTRH"
     gene            3519..4382
                     /locus_tag="PK264_01820"
     CDS             3519..4382
                     /locus_tag="PK264_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74666.1"
                     /translation="MGDAMFTNVPKTKIHYLLENPLPQAIAGHGGKVQTFANLVAIDR
                     SVLRYRLKNNDERLYGSEELERIARAHKFPPDWPEWTDHRRSIRSFVKRYERECGPSP
                     WAHNRDWVQLELEVRQGARDTVLFHLNCSTEIIPTDGNEEFSFAVTVKRGMVDYDLGV
                     AWTDREWTDLNLGAATIERKTTSVNRPRWQINTTERKIGMISTPDGFCPLSTVTDTKV
                     TATFTVEVKDLLVTPVLSGERSSAEKLTNAKKKFIARLAAEDLLDDNEVVTLCVDRRD
                     IKARSTSRSGA"
     gene            4379..6079
                     /locus_tag="PK264_01825"
     CDS             4379..6079
                     /locus_tag="PK264_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRD74667.1"
                     /translation="MKVPQKFDPSIMRRMTAVAECQADSFEALVRASQLDPQRHFRRA
                     DWHLLDFTGCDLRDFDFSHARLTACRFNGARIAAGDGKSAAKFERAEFDNGIVGVDLK
                     REDHPLRAAVDWAQFASDYRSPRHLPMMNIVTLGHLDHGKATLGAALTRVTADKGWTR
                     RAVSYDELLRASESQGRRDDTKILNIGGAIAEFCSLRYRYSNGIAPGHADYMKSALRL
                     AAGADGAILVVSTVDGPMPMTREHIIVARQMDIPKIVVFLNKCDIVDDVELLDLVEME
                     IRELLRIQNFPGDDVPCIRGAAVQALNGELGPLADEALLKLIGAMDEYFSPRPRKISD
                     PARLWIGDVYIKPGVGCIVTGVIDAGTIGCGDRMELLGYGKSAFVVVESIRIWDDAVE
                     AAQAGQFAGIRIRVLDEPICPQQAAPEPAAQKPGPPPIMLPPLAMPAPDFAAPIAPAS
                     TQAFDLDLCKPRRGMMLAQQGSVPLVMRVKAELYVLTKDEGGRHTPFFKHYKPQVFYG
                     TMRATGTIIWLDREMAMPGDNVSVEMSFEYGVPLVVGERFTLREGGRTIGSGIVKEAT
                     "
     gene            complement(6342..>6821)
                     /locus_tag="PK264_01830"
     CDS             complement(6342..>6821)
                     /locus_tag="PK264_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74668.1"
                     /translation="STELDHASTRDDLDALLAEDGLTLEPKGSGFVVGNAASYVKLSR
                     LPLQHGAKGLARRRPPPRSKRPKAVRRKPLSRNLWTVDAVDIARRLGTREQLRAAIQD
                     ARGHRKARLARAPLMTQLMEEMKETLKASTALSRKPQRPPRRRRPGDKQSRTDYRDR"
     assembly_gap    6822..6893
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6894..7427)
                     /locus_tag="PK264_01835"
     CDS             complement(<6894..7427)
                     /locus_tag="PK264_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HRD74669.1"
                     /translation="MLANIEPAHNDFLALAGYLVHGRDRPTHPARAAWTFGHNLPTDD
                     PLLAATLMGATAELSRRCRSACYHMSINWHPDEHPTPETMQEIARRTLDLAGLAEHQA
                     LVMGHGDKAHKHLHMMINRVHPDTGRAWSTSHDYRRLDRIMKLLSEEYGFRYVPPHSF
                     APELTDELPKPMVRVKGT"
     gene            complement(7430..7642)
                     /gene="mobC"
                     /locus_tag="PK264_01840"
     CDS             complement(7430..7642)
                     /gene="mobC"
                     /locus_tag="PK264_01840"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HRD74670.1"
                     /translation="MADILDAFAAGHRPELPQARGPSSAALRELTRIGNNLNQIAHRA
                     NLMRLRLIQADARKALAAVLAAVRRL"
     gene            complement(8333..8644)
                     /locus_tag="PK264_01845"
                     /pseudo
     CDS             complement(8333..8644)
                     /locus_tag="PK264_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8648..8911)
                     /locus_tag="PK264_01850"
     CDS             complement(8648..8911)
                     /locus_tag="PK264_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRD74671.1"
                     /translation="MKATAAEVGKNFGLFADKALVEPVVVTKYGRDHLVILSADEYAR
                     LKRQDRRVYRAHEVPEDILAAIEATEPPPESEAVERELAGKAR"
     gene            complement(8988..9395)
                     /locus_tag="PK264_01855"
     CDS             complement(8988..9395)
                     /locus_tag="PK264_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74672.1"
                     /translation="MIVTHTQNATGQRRVYLGAKASLECWIEPKADGTWSFHIADAVA
                     GIELGDATRRTSAIHTLAALADELAVAPADLAAVPFEVIAALHTASPYANRRVPLPRR
                     TPIENSFMATSPGITRPSADFRARDFGGFRRPR"
     gene            9797..10153
                     /locus_tag="PK264_01860"
     CDS             9797..10153
                     /locus_tag="PK264_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74673.1"
                     /translation="MTETEKNNRDSEVFSVVEGHKGYLREAYHGGPYVTRILLPEAFA
                     DEAVGDEVPIPAKLMQERLGEALSAAMLRQLKVYEEPMESGHTKAVCESFKEFVALCV
                     RKEAETGEPCRIYASY"
     gene            complement(10582..11427)
                     /locus_tag="PK264_01865"
     CDS             complement(10582..11427)
                     /locus_tag="PK264_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74674.1"
                     /translation="MIALRIASLIAALATIILNAHGGFTSTKVFEYAVLFAALYAALD
                     IGKCAVLVAASRAWRQRAYLVAALCTLLFPLLFANSVWNAIQQIAITRDDTKAVRVGA
                     TQTRARTETEHARHTAELALLHASPTYAATSACALPKSKDARTFCDRVAIATTAIARL
                     NAQLAASPPTDAEPHVTLFAAITGARPQAVQFTALLVPVLLAEVLGAVGFYIASRINA
                     EAPGTPARKNFVLRRGTWRPNRQKPSDENSGAAGTMPSRQPSGVVQTPTAPASPNITW
                     RLPSS"
     gene            complement(11424..12542)
                     /locus_tag="PK264_01870"
     CDS             complement(11424..12542)
                     /locus_tag="PK264_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74675.1"
                     /translation="MLREMIDPFKSGLAGALAPKPLEVDFHRLPMKARPYLLGPFLKL
                     NQGDRFPELDLEHSTATTLVMVHTGGPVDADLWRAHLPAINAWLNAGVFPFQWTITDH
                     SASILVITRQSQLPPSIPFDRSAHLTAGALFAGIDVTQRMPVHIPFAELSSGTYITGA
                     AGAGKTNAVHILLRSIFANIDLFAEVHLVDGKDGVAFMRYAGLHPKIRVLHDEPDVWA
                     LAQRLTVLMKERNIAQRRLGVDKATRDYIALVIDELPTYIAKPPKAQRAEHEAFLDNM
                     LKIAMRGRSAGIRMMLITQSPVADQIPVTLRTNCSTRIVFRLPEQSHAVSLFGELTPT
                     NDPRKLTTGQAIVDQSDTGRTTKVQFPFAPLFNPRGRP"
     gene            12630..12821
                     /locus_tag="PK264_01875"
     CDS             12630..12821
                     /locus_tag="PK264_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74676.1"
                     /translation="MPQEIETRIEDVAGLLVDLLRVFEAMVEIGERSGALAAGGDDDA
                     GALHVVVGDGHGRTFGRSL"
     gene            complement(12791..13093)
                     /locus_tag="PK264_01880"
     CDS             complement(12791..13093)
                     /locus_tag="PK264_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74677.1"
                     /translation="MRFLDRLFGSVTAPPATPAPVAPSLALKAAPTFHPVPANSVDPL
                     DAEVLRLWDRYQRGVADPTWDKFCSWLVEANIALDRDIAEVRRRLANYNDLPKVRP"
     gene            complement(13097..>13290)
                     /locus_tag="PK264_01885"
     CDS             complement(13097..>13290)
                     /locus_tag="PK264_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74678.1"
                     /translation="PIFDPIPPSPPVMAPPPRRYPQTVRTYTTPPPLPPGYVFKPVLE
                     IKPAKDPGLTFTIETSRKA"
     assembly_gap    13291..13406
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13407..13774)
                     /locus_tag="PK264_01890"
     CDS             complement(<13407..13774)
                     /locus_tag="PK264_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74679.1"
                     /translation="MTSLLPPKIALKLTPVPKPLLYAGFAGEFLKNTGMKDDGQIRAI
                     QQCVSEGLVAKVIVTAVHVDGRIEKYTLSLPPFGAGDTVMLALENGKSYLESLDVGLA
                     AAVQYAAEQIIRRGLTPRFS"
     assembly_gap    13811..13820
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13830..14792)
                     /locus_tag="PK264_01895"
     CDS             complement(13830..14792)
                     /locus_tag="PK264_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD74680.1"
                     /translation="MSTHFPNLAGTPATGHTVNGATPPHGPWQYAHLIRKPGDPIVRQ
                     RFEFRALSLTVREHLVNQIAEATRNDAARMLWVQGRPGEGKSLGCLVACLNAGFHTAV
                     LSPGLFAGDVEGASVQALHDVLTELVAWSAAHRCRVVVIIDDFDLSTANVGENQGSTV
                     NSQLLVNEFMALADNRHLYRNVDGSNIGFICTVNDATGMRESLHRPGRAIWHDHVPSA
                     EDKANIAWSALDPKTTPERELVAALVRKHAAQPVAFWNALHHRMRALHAKKLIDTGMP
                     DKAAVDAAHGHRLPLVSAIAWEAAKQIRTSRVRSYLGKRRWWRR"
     gene            complement(14803..15225)
                     /locus_tag="PK264_01900"
     CDS             complement(14803..15225)
                     /locus_tag="PK264_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74681.1"
                     /translation="MTTPKFSWPTGPVIEPKSAETLRQPALKDEKDVVRDALDELDEI
                     VRIAGGFPGQPAANADPAAAELFAPPPGSPARSSQASDDPYPDQPADLAVDASPPPLD
                     TAAERAAWSTLLALINAHKDPGTIVTAADPDTFMSGLE"
     gene            complement(15294..15599)
                     /locus_tag="PK264_01905"
     CDS             complement(15294..15599)
                     /locus_tag="PK264_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74682.1"
                     /translation="MATTLQGIVLFATFEKEDVFQYTDPKTNVVRPLRSLKVLLAHGD
                     GTVTRESISIGPDFNASALTPQKVFGFQTTAVVSKKTGRLSFTLVPNTPILPAPTFD"
     gene            complement(15668..16471)
                     /locus_tag="PK264_01910"
     CDS             complement(15668..16471)
                     /locus_tag="PK264_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HRD74683.1"
                     /translation="MIGPAKGRTVARLARRIEACATSFHVRVRTPRGEATEIITRNQR
                     DCRSRLCPISAAWSAATMRRKVQALIDEVFAARPDARALFLTLTLPAQPAVSASLKPL
                     KAALKRFRAIPDVIANTRGIFISIEVTVGGTPDAPTLHIHAHGLVIVDGGPGGYFKGG
                     VPRVHQTTWAQWWQTAARLDRKPVVDIRAARGPSGETDPASVRRSAAELAKYAISPAH
                     FAHDDNGITADPTIVLALHTLLKGQQLVSFDRCFKDAQQRLKTNPRNPS"
     gene            complement(16567..16827)
                     /locus_tag="PK264_01915"
     CDS             complement(16567..16827)
                     /locus_tag="PK264_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD74684.1"
                     /translation="MSANDTALPEFLGLPADDTVQLTTLEAARVLRVSTQTLERWRSQ
                     GIGPAHQKGPGLRSRVTYLAGELRAYVAASRRTSTSDRGGAL"
     assembly_gap    16862..16878
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17315..18100)
                     /locus_tag="PK264_01920"
     CDS             complement(17315..18100)
                     /locus_tag="PK264_01920"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRD74685.1"
                     /translation="MTRKFNEIALAHKLSEIEPEISADVVHTTNDQAQRDQSASAHPT
                     VQAYGPLIVAYDHFNRELFGGMLPACIITLQRNAERVLGYFHGDRFGHRERSNLTVDE
                     IAMNPRHFRRRSIEETLSTLAHEMVHLRQHHDPEGKPSRVGYHNKQWSQWMQDIGLRP
                     THDGTPEGRLHGQRMTHIIVAGGAFDIACRKLLSDQFRIAWYDRLTEMSRVSEGGAEN
                     PGSLPAPSAGKRTKFTCPQCGSNAWGKPSLNLICGDCRVKYEA"
     gene            complement(18212..18406)
                     /locus_tag="PK264_01925"
     CDS             complement(18212..18406)
                     /locus_tag="PK264_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74686.1"
                     /translation="MRHPRNPEAIKALYQALSSGYRTPREERVIAKWLAYLLDNQPPA
                     LPGRGAEIKPETSDEAALAA"
     gene            18659..19939
                     /locus_tag="PK264_01930"
     CDS             18659..19939
                     /locus_tag="PK264_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74687.1"
                     /translation="MWALRQYENRVLQAQEDIAAETGALKKEATTPWSNSRLRRRSDR
                     VFGRESGRLPSERFTGADHEKMKDSLRMLADGVEKQSRLEDHLEAIEQLFVEYEQKVI
                     LIQQRVPKLFKDNMRRRGIISIKDLLTPEGFNKGIYAPIYEAMIAGRFGECIDNLSQS
                     LSALANDATLKSSKFSGSILAATLSISFYCLHRMGHPGQAELLEYLVRMQSQDIAEED
                     RKSLRMALLAYASYEDPHSKSARQARELAGPSGLKARWEPGIAGCELRLALDYGLAKR
                     LAPDEPMRDLERANIRSVVKAHEALGDNVLSGSYWKPKTALVSAYAHHGGLDDVIACA
                     YAIRLEMLKKNIDMPHVEIRLLVDEARVLWEKSSSKKCHLAVMAAEKVMAARRISGRT
                     GIPLVHGARTAYVQSVIDEIQLDEHTHGSNRRVA"
     gene            complement(20848..>21836)
                     /locus_tag="PK264_01935"
     CDS             complement(20848..>21836)
                     /locus_tag="PK264_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HRD74688.1"
                     /translation="NRALRALGNAIKHADRRQDILGALATDLPAPVLRTPPADDGQLS
                     FFVPHIYDAPIKDDMNLMDIAPFSLAKGRRDGVITYELKDCTITIDGSAGAGLATAFD
                     YDIFLHMVSHMAEEARRYHLAINKGQRPHLPARIYRPNVAHILKFCRRNSGGKQYRDV
                     ESALDRLAGTRIKIINLSNGRRREVLNMPLIQNFKVISKTTSGHVDQVEIAVPDWVYD
                     GVVNTNATPHILTINPDYFLISQSIGKVIYRLARKAAGKTRAEYGLTELHKRSGSSQP
                     KPQFAQMLRRFVASTKLFQFPDYDLELADGLTEPKLVMQYRASVLHLTPSPI"
     assembly_gap    21837..21871
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21872..22051)
                     /locus_tag="PK264_01940"
     CDS             complement(<21872..22051)
                     /locus_tag="PK264_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74689.1"
                     /translation="MTAQTAPKLDLISQGEFAALSLHQKNDYLHDLARRFAEATNRPP
                     TPLTPEGLARLRRFYA"
     gene            complement(22048..22371)
                     /locus_tag="PK264_01945"
     CDS             complement(22048..22371)
                     /locus_tag="PK264_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74690.1"
                     /translation="MNSPVFRPIGAPLDVPDTALDTLNRQLGVPTLVPPVTRDAPPAE
                     PRSLTKAINIHVPDYAVAALKAKAFEADSSVRFIVLKALDQFGIAIRPADLVEDARRS
                     DRTEP"
     gene            complement(22368..23042)
                     /locus_tag="PK264_01950"
     CDS             complement(22368..23042)
                     /locus_tag="PK264_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD74691.1"
                     /translation="MHITFASSKGGVGKSTACACLAGALVARGHTVHIVDLDNNHTVS
                     RWLSRSKGFSVSTPDPLALTEHLDMLAKKSLDFVLIDVAGAYERAITVAMARAHLTII
                     PAAPTEADLFEANRIATHLRSVFAAFNREPLFRLLLTQVQPLASHAQSHAIREVDRLA
                     LPRFYTMLTHRAAYQEIGLSGSPPHQSNRTRSTTAKAVAEIDQWLAEIALLLPTQQPI
                     NRKDAA"
     gene            complement(23219..23518)
                     /locus_tag="PK264_01955"
     CDS             complement(23219..23518)
                     /locus_tag="PK264_01955"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD74692.1"
                     /translation="MTAPARADIDACLAYIAREAGLDIALRFADDIDAELSRLAWIGH
                     SGASREWLSPGLRLHVLGNYCIYFRVTPTETRIIRFLHGAMDISRLSFDDTSSNP"
     gene            complement(23613..23888)
                     /locus_tag="PK264_01960"
     CDS             complement(23613..23888)
                     /locus_tag="PK264_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HRD74693.1"
                     /translation="MQHRKTISLTAKDDAFIAAQLDSGEYGNASEVIRAGLRLLEREH
                     LKLAELRNAIAEGDAAYARGDVTRYTEPGQLAANLKAKLLKDHTPER"
     gene            complement(23939..>24148)
                     /locus_tag="PK264_01965"
     CDS             complement(23939..>24148)
                     /locus_tag="PK264_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD74694.1"
                     /translation="RLHDLRHTWASTALISGASLAVIGRQLGHMTPRTTARYAHLADD
                     PLRLLAQQTGETLAAALNGNPTKAA"
     assembly_gap    24149..24158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24174..25181)
                     /locus_tag="PK264_01970"
     CDS             complement(24174..25181)
                     /locus_tag="PK264_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD74695.1"
                     /translation="MPKPIRKKITLTVINSVTPNTFIWDTQLRGFGIRRQRSDAVTYI
                     LRTRIHGRQRFFVIGQHGMATPDGNTWTPDTARRQAVKVMGNPAIAEKPTKSKPDQQP
                     GPLTFENVAVLFLAKHGPMLRPRTRVEYERLIRLYLNPAFGAKPLTAITRADVSTASL
                     AWSDTPRSANFALTVLSKLMTWSKDEGLREGDNPVTRVKRYKEVRRERYLSLDEVSRI
                     GAALADCERDGSINPHGTAAIRLLILTGARLSEILTLQWSFINLDRGLLFLPDSKTGQ
                     KTITLNDAAIAVLREVPRLAGNPYVIVGKIHATHLANLHYAWDTLCERANITGGRRQS
                     R"
     gene            complement(25668..26360)
                     /locus_tag="PK264_01975"
     CDS             complement(25668..26360)
                     /locus_tag="PK264_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD74696.1"
                     /translation="MTTHRVLVVDDEPQIQRFLKPSLSAAGYEVIAAVNGAEALKAAA
                     TRAPDVILIDLGLPDMDGKDVIAQLRTWSKVPIVVLSARDREAEKIAALDLGADDYVN
                     KPFGIGELMARLRTALRHAAQSMGEVSRIASGDLVVDVAAHVVSRAGTPIKLTPKEFE
                     LLAILARNAGRVVTHRQILTAVWGPAHTEALQYLRVFIGQLRQKLKGDPNDPDLIQTE
                     AGIGYRLAMGAD"
     gene            complement(26360..>28138)
                     /locus_tag="PK264_01980"
     CDS             complement(26360..>28138)
                     /locus_tag="PK264_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRD74697.1"
                     /translation="RLGGTTVRLSATDLAGEILAYAKRNNVTQIIIGRSKAGPLARLL
                     GRSLSRDLVRRADGLSVLIVAPESEKPSKAGASAQPRSALPPAGTLAAALAIAGGAVT
                     AAVLAGIALEQITRLPNLSMVFLLAVLLCGMRFGMTSAIAAAVLSFLAYNFFFIDPRY
                     TFTITQPYELLSLFMFLAVAVVTGSLAGRLSEQATATRERAEATQALFDFSRKLSGVS
                     KLDDVLWLLASQVAAVAKGKSVVLLDRSDGLTLENSWPPDDQLGTSDWAAARWAHEKQ
                     EPAGRSTDTLPSARFQFRPMQGSRGSIGTVGIDAGDADDALSDAVIATIQSFIEQAAV
                     AIERIALVEQATRAETAAAGERLRAALLSSISHDLRTPLASIVGSVTSLRTLGDRMSQ
                     LDRGDLLATIEEEAGRLSRFVSNLLDMTRLEAGAIDIRRDWVDVGDVVASAVERARKA
                     FPRRKIATAVSEKIPLIRGDAALLEQVLFNLLDNAHKYTDQGSQTELKVAAESAGITL
                     TVTDRGIGIPPEELERVFDKFYRVAGSDGRAPGTGLGLSICAGIVKGMGGIIRAESPV
                     AGNRGTRITIMLPVAANRGTEEAAGQ"
     assembly_gap    28139..28340
                     /estimated_length=202
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28341..>28730)
                     /locus_tag="PK264_01985"
     CDS             complement(<28341..>28730)
                     /locus_tag="PK264_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HRD74698.1"
                     /translation="TNAPGLIHAKRYQDVEEALRNNIDVWTTLNIQHLESLQDVVQKI
                     TGVRVQETVPDKVLEKADEIVVVDLPPEELVQRLKEGKVYLPDNARRAIDQFFKPSNL
                     TALRELALRRTADRVDEQMLQQLRQQG"
     assembly_gap    28731..28746
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28747..29084)
                     /locus_tag="PK264_01990"
     CDS             complement(<28747..29084)
                     /locus_tag="PK264_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HRD74699.1"
                     /translation="MADPNTKSPNERRPSPKALLELAAKEGRGKLKVFLGMAPGVGKT
                     YAMLSAARAQKAEGVDVLVGVVETHGRSETAALLEGLEVLPRKPVQYRRSTLMEFDVE
                     EAIARKPKLLL"
     gene            complement(29091..29669)
                     /gene="kdpC"
                     /locus_tag="PK264_01995"
     CDS             complement(29091..29669)
                     /gene="kdpC"
                     /locus_tag="PK264_01995"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HRD74700.1"
                     /translation="MLTLLRPALVMLALFTLVTGVAYPLAMTVLAQALIPATASGSVI
                     TRGGTIVGSDLIAQSFTSDRYFRPRPSAAGEKGYDATGSSGSNLGPLSKKLIERVEGD
                     VATLRKAGASIIPADAVTTSASGLDPHISPAFAGLQIARVAAARNVTAAQIRAIVENS
                     TERPLLGLFGEPRINVLRLNLTLDASLPGGAG"
     gene            complement(29681..31729)
                     /gene="kdpB"
                     /locus_tag="PK264_02000"
     CDS             complement(29681..31729)
                     /gene="kdpB"
                     /locus_tag="PK264_02000"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRD74701.1"
                     /translation="MSTKAIPLLDTGILRQSAVDAFTKLAPAKLVRNPVMFVTAVVAL
                     LVTLLFVRDLMSGRGGLLFSGQIAAWLWFTVLFANFAEAIAEGRGKAHTDALRSTRSD
                     TQAKLFLHPDDPKDDLWEPKAAELLEVGDVVLVEAGDTIPADGEVIEGIASVNESAVT
                     GESAPVIRESGGDRSAVTGGTLVISDWIKVRVTAEPGSSFLDKMIALVEGAQRQKTPN
                     ELALSILLSGMTLIFLIAVVTLWGLAGYSGTALSITVLVALLVTLIPTTIGGLLSAIG
                     IAGMDRLIRFNVMATSGRAVEAAGDVDTLLLDKTGTITYGNRLATSFIPAPGASEQDV
                     AAAALLSSLSDETPEGRSIVALAKGTYGQMESERSGRATYIPFTAQTRISGIDVDGRI
                     IRKGAVDAVLKSIGTTIEDAPAVFREAVDSIARSGGTPLAVADGPRLLGVVHLKDVVK
                     PGIKERFTALRAMGIKTVMVTGDNPVTAAAIASEAGVDDYIAEATPQDKLAYIKAEQA
                     KGRLIAMCGDGTNDAPALAQADVGVAMQSGTQAAREAGNMVDLDSNPTKLIEVTEIGK
                     QLLMTRGSLTTFSIANDVAKYFAIIPALFLVTYPELGALNIMGLATPESAILSAVIFN
                     ALVIVALIPLALKGIAYRPIGAAALLRRNLLVYGLGGLIVPFVGIKVIDLVVSALRLA
                     "
     gene            complement(31742..33451)
                     /gene="kdpA"
                     /locus_tag="PK264_02005"
     CDS             complement(31742..33451)
                     /gene="kdpA"
                     /locus_tag="PK264_02005"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRD74702.1"
                     /translation="MTLIGWLQIALVLGGVLACAWPLGRFMAQVFQGERTLLTGVLKP
                     IENALYGAAGIKAEKEQSWLAYTLAMLMFNALGFLLLYALMRLQAILPLNPQGFGAVP
                     SDLAFNTAMSFTTNTNWQAYGGETTMSHLVQMAGLTVQNFLSAATGIALAIAVTRAFA
                     RSSAQTLGNFWVDVTRATLYVLLPLSIVVAIAFALAGVPQTLAGSVEVTTLEGAKQTI
                     ALGPVASQEAIKQLGTNGGGFFNANAAHPFENPNAWSNVLSIWSMLLISTALTFTFGR
                     MVGDRRQGIALLVAMYGLLITATAVVYWAETSGNPLLAKAGVDMTAGNMEGKEVRFGQ
                     ALSALYVVATTGLSCGAVNTMHNSLMPLGGLVPLFLIQLGEILPGGVGSGLYGMIVIA
                     ILTVFIAGLMVGRTPEYLGKKIEAREMKLAMLALLILPLAILGFTAASAMLPKALESL
                     ANAGPRGLSEILYAYSSAAGNNGSAFAGLSANTSWYNTTLGIAMLLGRFAYVVPVMAI
                     AGALAAKKKVPASSGTFPTHGPLFIGLLIGVILILGGLQYFPALALGPIVEHFLMHAG
                     KTF"
     assembly_gap    33547..33603
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    33950..34008
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            34009..35177
                     /locus_tag="PK264_02010"
                     /pseudo
     CDS             34009..35177
                     /locus_tag="PK264_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612078.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    34227..34343
                     /locus_tag="PK264_02010"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(35251..36063)
                     /gene="xth"
                     /locus_tag="PK264_02015"
     CDS             complement(35251..36063)
                     /gene="xth"
                     /locus_tag="PK264_02015"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRD74703.1"
                     /translation="MLIATWNINGVKARIETLLTWLGERNPDVVCLQEIKSVTEGFPA
                     AEIEAKGYNVAVHGQKGFNGVAILSKRPLEDVTRGLPGNDADEQARYIEATISVPAGA
                     VRIASLYQPNGNPIGTEKFSYKQAWAARLEAHARHLLALEEPLVLAGDYNIIPEPADA
                     KSPGAWTEDALYQPESRAAYRRLVALGLTDALRACEPGPGVYTFWDYQAGAWQKNNGI
                     RIDHLMLSPQAADRLERAAVDKHVRGWEKPSDHVPVWVKLDLTAVPTASRVA"
     gene            36204..37049
                     /locus_tag="PK264_02020"
     CDS             36204..37049
                     /locus_tag="PK264_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD74704.1"
                     /translation="MLDLAGASGSGRALLLLAIALLIGGLVTGFLSGLLGIGGGGIVA
                     PILYEVFGIIGVDPAVRMHCAVGTALAVMIPTSIRSFMAHKARGGVDMEAWRRLGVGV
                     VAGVVIGALVASVSPGTLLKWVWVGFATVMSLKLVFGKEQWRLGEQLPNAPLTELYGA
                     FVGVISTLLSIGGGAFINTFMTLYGRTIQTAVGTASGIGPLIAIPGTIGFLWAGLGVA
                     GRPELSLGYVNLVGFVLVGATSVLAAPWGVRLAHGVSRRALELAFAAMLSTIGLRFLW
                     TLLFE"
     gene            37089..>37385
                     /locus_tag="PK264_02025"
     CDS             37089..>37385
                     /locus_tag="PK264_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74705.1"
                     /translation="MAQTILRRPATYADLEALPDNMVGQILFGTLHAHPRPTPRHIRA
                     STKLGAELDGPFDRGRGGPGGWLLLIEPDLHSGEHVVVPANYNSPGQIVIGGHAL"
BASE COUNT         6758 a        10369 c        12762 g         6902 t
ORIGIN      
        1 aaggaggaag ggctgccgaa actgatcagc ggcgcgtgga ccggtacgat gaacctcacg
       61 gagccgcagg cgggatcggc gacgatggcc gcatcgggga tctccggcat ggtgatggcg
      121 gccatcagtt cgacgaccgg ctcgggcgtg aagaactggc cggcataggc attcgccacg
      181 gcctgctcct cgtagacgcg gccgaggtag tcggcgggct cggcctgcat cgcggccatc
      241 caagtgccga gcgcgcgggc gaagtggtcg gccggatggt ctgtcgtgcc catcggcgcg
      301 gtgcgcgggc cgtagcgtcg cacgatggcc atataggcgt cgtcatcgcg gaggaacgca
      361 ttgacggcga gcgccagcca atcctcgaac gtgcggaaga agtgctggtg cgtgatgcgc
      421 tggagatggg cggcgatcgc ggccggtgcc ttggagggcg tgagcggggc cttgctcacg
      481 cgtcgcggcc ctcgtcgggg acgagccggt cgaacaagtc accctggcgc cagaggaact
      541 ccgccagctg atagtcccgg ctccgggcct cgcggtccat ccaggtggtg aagaacgtgg
      601 tcggacctcc ggcgcccgtg agtgccgctg tggagaggaa atgcgcgagg aaccccgtgg
      661 tggtgatcgg cacgggcgta tcgcgggcgg cgatgacgtg gagctcgagc agagtgtcgt
      721 ggtcgccgag gtcgcagggc ttctcgacga ctttgcagcg caagtgccgc caggtgaacc
      781 aatgcgtgcg gaccggtcgc gagctcatcg catggggccg ctgtcgtgtt ggcgcgggtt
      841 tcggaaagcc tctgaggcgc ccacacgcgc gatgatggcg tcgtggccga ggtcggcgcg
      901 accgacgagg cggaaatggc gatcggcgat gcgaacgtag atcgcagaga tcggccccgc
      961 tgtcggttcg atcgccttga aactctcgcc ggccgccgtg ttgcgccagt catggggcgg
     1021 cagagcgttg agcatctcgt ggaacaggcc ttcggcgatc tcgacgggct cgtcatggac
     1081 ggtgtcgatg atccggcact ggatggaagc gaaggcagtc ggggtcgcag gggtggtggg
     1141 cacggccgca gcctgcggga ccgtgcgtgc acggtcaata cacgctgccc atcgcggcgg
     1201 cagcgtcgtg cggggatcgg acggggcgga accgcgcacc ggaggcccgg ccggacggcg
     1261 gccccttgcc atttcttcgg agccatccgt cgcggacccg catcccccgg ggcgcctcag
     1321 cgcgcgggtc cgcgagggat ggcggagagt ggcattggcc ctgccatttc ttgggtgaat
     1381 cccgagcgac cttgccgggt gcaccgcagc ggcgggcgtg ccagggcgac gttgacgtca
     1441 acgtcctggg gtcccccgac attccggctc tcacgccccg cacgcacacg agtgctcacc
     1501 gggcgggcaa ccggcttgat gcaccatggg tgacggcgcg agacttcccc catgccgcgc
     1561 tttcaacgca acaccgacga cctcggcgtc accgaggagc actaccggat gttccggagc
     1621 ctggtgcccg acatcatcgg cgacatggac cagaagcgcg cggcggccat cgaagccgcg
     1681 aaaatctatg agcgggcctt gaagcgcggc agccgggccg ctgccgagca ggcggcgcgg
     1741 gactggctcg ccgagctgca ggagcaggtg ggcgcccagc acggcatggg gcgatagctg
     1801 cgtcaaaccc agacgacaac gaccgccgca gcggtcgtca gatcagagca accgatcagc
     1861 gactgtcgcg ggcgtcggcc ttgcggcgcg ccggagcttt ttcggcggcc ttggcgggct
     1921 gcggatagct cgtctcgccg cgcaggatcg ggcggccgta gcggtcctgg ccggcccaga
     1981 tgccgacctg gaacacgggc gagccgtcgc cggggttcac gcgggcccag aagtcggggc
     2041 gcttggcgcg ggtcgcagcc tcgtccttgc tgtcggtgtc gacgggctcc ttcgacttgt
     2101 tggcgaaggc gaccatctgg aaggggcgca aggtcagccc ggggccggcg tcggccatgt
     2161 cggtggcgtc gtgaacgggc ctgtcggtga ggctctcgag ctgggccatg acgttgtcgg
     2221 tgatgggggt cggcgtgatg cggccggaga agtagagttt gccgtgcctg tcgcggccgg
     2281 cccacagcgc cacggggaac tcgtgctcac ggcccgggat gccgatgcgg cccgtgaagt
     2341 cgggcgtgtt ggtgcccggc tgcttggcgc ggttctgtcc gacgatgaag ttgccggtct
     2401 ctgatgggtt catgggtcct cctcggttgt tggtgacggg aggcagcgtg cggcagcgtg
     2461 gatgcgaaag caatacgggg tgcccgcgca aacggcagcg acgtatgcgc ggtgctcagt
     2521 tgcgaggcgg gtgcgaggtg gtatggtcgg gggtgtcggc tatgtggggt gcagcgacgt
     2581 tgggcgagga gaggctgcgg gggcgtgatt gaccctgagc tgccctcgtg caggtgcgca
     2641 acactctcgt ggcactacgc cgaggctgcg gatcatcggg ggcagaatgc tcgccactcc
     2701 cagtggatcc taccgtccgg gagcctcaaa tttctctgct ggaagactgg tggaacttga
     2761 atgcagcagt gcgatcgtcc atccggcaac ctcatgcaat gccgctgtgg cggcggaggg
     2821 gtgtgataca ccttcgccgg cacattggtg gttcgcggtg accgttcatc cataaccgcc
     2881 ttcacgacga agggcaccaa agccgcagcg gcagcacatg cgatcttggc aaggcaggat
     2941 tcattgcagg acatcgaggt ctccgtgctc aatgccgggt gaagtagcga ccaagggctt
     3001 tgccgccctt gatgagggtc ttcacgcgct cgtcctcggt catgacgagg atcaatttgg
     3061 ccagcccatc ggcctgagat gcggagataa cgccggcccg cctcaactcc atgcagcgcc
     3121 ggcgcgagat actccaggcg tagtcgccgg aacccacata gacgtcgcga tcaaagtgct
     3181 cgagcaggag atcgacgagc aagtcgccct tgcaacgctc gttcttccgc tgccagccgt
     3241 ggtccgtata agcgagatgg ggcatagtac gcgctcctaa ttcaacagct aacgtcagcg
     3301 gaggacgcta gttggtcgat agaagaagca ccacgaatgc ggctaccagc cgtgacgcga
     3361 tatttccatt gctgtatcag ccacttgatc gcgtcgatcg tgtctgaagg catgtgctgg
     3421 caggatgaca tgggggagtg gcagtttctg gtagtcaacc tggttgtgct gagctgtacc
     3481 atcagcttgt ctgggcgctg catccacgga gccgtgcaat gggcgatgcc atgttcacaa
     3541 acgtgcccaa aacaaagatc cactatctat tggagaatcc gttaccccag gcgatcgctg
     3601 gtcatggggg taaggtccag acgttcgcaa atctggtcgc catcgatcgt agtgtcctca
     3661 ggtaccgctt aaaaaacaac gacgagcgcc tgtatggctc agaggagttg gagagaattg
     3721 cacgcgccca caaatttcct ccagattggc ccgaatggac tgatcatcgc aggtcgataa
     3781 ggtccttcgt caagcgctat gagcgcgaat gcggtccgag cccctgggca cacaaccgcg
     3841 actgggttca actggagctt gaggttaggc aaggtgcgag agacaccgtg ttatttcatc
     3901 tcaactgttc gactgaaatt attccgactg acgggaacga agagttttcg tttgcagtta
     3961 ctgtcaagcg aggaatggtc gactacgacc ttggcgtggc gtggacggat cgagaatgga
     4021 ccgacctgaa tctcggcgcc gcaacaatcg agagaaagac caccagtgtc aatcgaccac
     4081 gctggcagat aaatacgacc gagcgaaaaa tcggcatgat ttcgacgccc gacggtttct
     4141 gtccgctatc aacagtcaca gacacgaagg taaccgcgac gtttactgta gaggttaagg
     4201 accttttggt cacgcctgta ctgagcggcg agcgatcgtc cgcagaaaaa ctaacgaatg
     4261 caaagaagaa attcattgct cggctcgcag cagaagacct ccttgacgac aatgaggtag
     4321 taacactctg cgtggatcgc cgtgacatta aggctcgatc gacttcgagg agtggggcgt
     4381 gaaggtgccg cagaaattcg atccgtccat catgcggcgc atgacggcgg ttgccgagtg
     4441 ccaagccgat agtttcgagg ctctcgtacg agcatcgcag ctcgatccac agcggcattt
     4501 ccgccgtgcg gattggcatc ttcttgactt tactgggtgc gatctgcgtg acttcgactt
     4561 ttcccatgca cgcttgacag cgtgccgctt caatggagcc cgcatagccg caggcgacgg
     4621 caagtcagct gcgaaattcg agcgggcgga gttcgacaac ggtatcgtgg gtgtcgatct
     4681 gaagagagag gaccacccgc tgcgcgccgc ggttgactgg gcgcaatttg cgtcagacta
     4741 ccgctctcct cgccaccttc cgatgatgaa tatcgtcacg ctcggccacc tggatcacgg
     4801 caaggcgacg ctcggcgctg cgctaacgag ggtgacggct gacaaaggtt ggactcgccg
     4861 cgctgtcagc tacgacgaac tcttgcgcgc ctccgagtcg caagggcggc gcgacgacac
     4921 gaagatcctc aacatcggcg gcgcaatcgc tgagttctgc tctctgcgat accgctatag
     4981 caatggtatc gcacctggtc acgccgacta catgaaaagc gctttgcgac tcgcggcagg
     5041 cgctgacggc gccatcctag tagtgtcgac cgtcgatggg cccatgccga tgacgcgtga
     5101 acatattatt gttgctcggc agatggacat ccccaagatc gtcgtgttcc tcaacaagtg
     5161 cgatatcgtc gatgatgttg agctgcttga tctggtcgag atggaaatcc gcgagttgtt
     5221 gagaatacaa aattttccgg gtgatgatgt cccttgcatt cgtggtgcgg cagtacaagc
     5281 gcttaatggc gaacttgggc cgttggctga cgaagctcta ctgaaattga ttggcgctat
     5341 ggatgaatat ttctcaccgc gcccgaggaa aatttctgat cccgctcgtc tctggattgg
     5401 tgacgtttat ataaaacccg gtgtcggctg catcgttacg ggagtgattg atgccggcac
     5461 tattgggtgt ggagacagga tggaactact tgggtacgga aagtcggcat tcgtggtcgt
     5521 cgagagtatc cgaatatggg atgatgcagt cgaagcggcg caggctggcc aattcgctgg
     5581 catccggatc cgggtactcg acgaaccaat ttgtccgcag caagctgcac cagagcctgc
     5641 ggcgcagaaa cccgggccgc cgcccatcat gctgccgcca ctggcgatgc cagccccgga
     5701 ctttgcggcg cctatcgcgc cagcgtcaac ccaggccttt gatctggatc tgtgcaagcc
     5761 tcgccgcggg atgatgctcg cgcagcaagg tagcgttcca ctcgtgatgc gggtgaaggc
     5821 cgagctttac gtgctcacga aagatgaagg gggacggcac acgccgttct tcaaacatta
     5881 taagccgcaa gtgttctacg ggaccatgcg tgccaccgga accattatct ggttggaccg
     5941 ggaaatggcg atgccaggag ataacgtaag cgtcgagatg tcatttgagt atggcgttcc
     6001 gctggttgtc ggtgaaaggt ttacgctccg cgaaggtggg cgcacaattg ggtccggcat
     6061 cgtgaaagag gcgacgtgat ggtttgcatt ctcggcgggt cgttgtccta aagccgcggt
     6121 tgccgcagcg cagcagaaaa ctgcgcgttc cttcttcact atcggcacat acaaagctca
     6181 ggctgccgga ttacgacaga gctgcgacgt gtcgtgccat gtgccttgaa gagcacctgg
     6241 aatctgatcg tccttgggcg acgtagtcag ctcgtggccc acagcgcaag tctgtggtct
     6301 atcgtgaact ttccggtttc tggggtatcg cgacggccag ctcatcggtc acgatagtcg
     6361 gtgcggctct gtttgtcacc gggtcggcgt cggcggggcg gccgttgcgg cttgcgcgac
     6421 agggccgtgc tggctttcag cgtctccttc atttcctcca tcagctgcgt catgagcggc
     6481 gccctggcga ggcgggcctt gcgatggccg cgcgcatctt ggatggcggc gcggagctgc
     6541 tcgcgggtgc caaggcggcg ggcgatgtcg acggcgtcga cggtccagag gttgcggctt
     6601 agtggcttac ggcggacggc tttgggccgc ttcgatcgtg gcggcgggcg ccggcgggcg
     6661 aggcccttcg cgccgtgctg caacgggagc cgcgagagct tcacatagct ggcggcgttc
     6721 ccgacgacga agccggagcc cttgggctcg agcgtgaggc cgtcctcggc gagcagggcg
     6781 tcgaggtcgt cccgggtgct ggcatggtcg agctcggtgg annnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagtgcct
     6901 ttcacgcgca ccatcggctt gggcagctcg tcggtgagct ccggtgcgaa actgtgcggc
     6961 ggcacgtagc ggaacccgta ctcctccgac agcagcttca tgatccggtc gaggcggcgg
     7021 tagtcgtgcg aggtcgacca ggcgcggccg gtgtcgggat gcacgcggtt gatcatcatg
     7081 tgcaggtgct tgtgcgcctt gtcgccgtgg cccatcacga gggcctggtg ctcggcaaga
     7141 ccggcgagat cgagcgtcct gcgggcgatc tcctgcatgg tttccggcgt cgggtgctcg
     7201 tcgggatgcc agttgatgct catgtgatag caggcactgc ggcagcgccg cgacagctcg
     7261 gcggtggcgc ccatgagcgt ggcggcgagc agcgggtcgt cggtcgggag gttgtggccg
     7321 aaggtccagg cggcgcgcgc gggatgggtc gggcggtcgc ggccgtggac gaggtagccg
     7381 gcaagcgcca gaaaatcgtt gtgcgcgggc tcgatgttgg ccagcacgtt cagaggcggc
     7441 ggacggcggc caggacggct gcgagcgcct tcctggcatc ggcctggatc aggcggagcc
     7501 gcatcaggtt cgcgcgatgg gcgatctggt tgaggttgtt gccgatgcgg gtgagctcgc
     7561 ggagcgcagc gctggagggg ccacgggcct gcggcaattc ggggcggtgg ccggcggcga
     7621 aggcgtcgag gatgtcggcc atgaggcgcg acagtgtcat cgatcgggct tgggcttcgc
     7681 ggtcgagcgt ggcccgtgtc gaggctgacg cgcgacagcg cagcaggtgt gggcgcggtg
     7741 tcttgtcgtc ggattgacct ttgcgatagc cccgcgatgg catgtggttg ctccccgttc
     7801 agtggcgcca gggagcttcg acgagagtgt tgcaccggcg cacggtgctc accttgaggg
     7861 gcggtcggcc attttgtggc cgaacgcata cctcgctcac ttctttttct cttcggcaac
     7921 cgtattcgac cggtacgcgc tcgttcaatg tcattgcctt ttaggaaggc acgttttcat
     7981 ctgtgatgtc acaattgcga tttagaaagc cggatgtttc acggaggcct tcggccaatt
     8041 gagagccagg ggctctgccc cttgcaaccc cgaatgcgga tcgggacaaa gatggcatgt
     8101 tgctggagaa agaggcagct tggttgccgg tggcgcattg gcggatagaa tgatcaggac
     8161 gggtcggcgt aatgccccgc accgttgaca aagatggcgc cccgacgggg cgcctttctg
     8221 tggaatgatg gcgcaggcgt ccggcacgaa tggcagcggc ggcacgatcg cgaacggtgc
     8281 ggaagaaccc ggcaggtaga tagccgaaca ggatcgtgtc ggtgccggta tggacaggcc
     8341 tcaggtcagg cccgggccat gcaaacacgt tcatttcgtc gaggatgatc cacgagcggt
     8401 cgtcgtcgag gcccagatgg cgcttgatcg ccgccggaat ctcgatcgca tcagtcgctg
     8461 catggggcgg cgtgtgcgtg atcggcagga cagccacgcg cgtgctgtcg gacacgtctt
     8521 ccagcgtgag cacgatggcg accggccggt ctttggtgcc ttcctcgcgt ccggcctcat
     8581 gctcgtcggc ccagaggtaa gcgtagcgga tgacctgggc gggcttgggc gccgggatgt
     8641 ccatgcgtca gcgggctttg ccggcaagct cgcgttcgac ggcctcggat tcggggggcg
     8701 gctcggtggc ctcgatggcc gcgaggatat cctcgggcac ctcatgggcg cggtagacac
     8761 ggcgatcctg gcgcttgagg cgcgcatact cgtcggccga gaggatgacg aggtggtcgc
     8821 gtccgtactt ggtgacgacg acgggctcga cgagggcctt gtcggcgaac aggccgaagt
     8881 tcttgccgac ctcggcggcg gtggctttca cggggtccat tctccgtact ttccttatat
     8941 tccgtattat ggggaacatc cgacgcgcgc gcaagggggg agccctgtca gcggggccgg
     9001 cgaaagccac cgaagtcgcg ggcacgaaaa tcagcgctcg gccgcgtgat gccggggctc
     9061 gtcgccatga agctgttctc gatgggcgtg cggcggggca ggggtacgcg gcggttggcg
     9121 tagggcgagg ccgtgtggag cgcggcgatc acctcgaagg ggacggcggc cagatcagca
     9181 ggagccacgg ccagctcatc ggcgagggcc gcgagcgtgt ggatcgccga ggtgcgccgg
     9241 gtggcgtcgc cgagttcgat gccggcgacc gcgtcggcaa tgtggaagga ccaggtgcca
     9301 tcggccttgg gctcgatcca gcattccagg ctcgccttgg cgccgaggta gacgcggcgc
     9361 tggcctgtcg cgttctgggt gtgggtgacg atcatggggt gagcgttggg cggacaaagc
     9421 ggggctggca agggagttgg tgaggagatg agcggaaagg tagctaagga ggagattgga
     9481 agggaagggg tgttgcaggg cggccagttc ggtgggttgg tgtttttcga gaggaggagg
     9541 gattgcacgg gacagaaaat tccggcaaaa taatgccgtc gatccgatac ggcttttcaa
     9601 aggattgacc gcgaggcgct ggtgtcgaga ccgagcgcct ttttttattg gctaacgtaa
     9661 gaacgaaaga agcagggtac attacgaaag attttttcac cctcgacagg tgagaaaagc
     9721 cgaccggcgg gcatcagacg gtaaccaatg aaagcccaaa acaatgggaa tcgatgtgta
     9781 tgcgtcatgg aaaggcatga ccgaaaccga aaagaacaac agagacagcg aagtgttcag
     9841 tgtcgtggag ggtcacaaag gctatctccg cgaagcgtat cacggcggcc cctacgtcac
     9901 cagaatcctg cttcccgaag cgttcgcgga cgaggcggtc ggggacgagg tgcctattcc
     9961 cgccaagctc atgcaggaac gcctcggcga agccctttca gccgccatgc tgcggcagct
    10021 caaggtctac gaggagccga tggagagcgg gcacaccaag gcggtgtgcg agagcttcaa
    10081 ggagttcgta gccttgtgcg tgcgcaagga agcggaaacc ggcgagccct gccgcatcta
    10141 tgcgagctac tgaagaggcc gccatggaaa accttgggct gctccttgaa gcgtccggcg
    10201 tgattggctg gacgtacctc gcatggcgcg tgaagcacgg cacgtcggac ttgctgcggc
    10261 ggttgcggtg ggcgttgtgg cccaaacacc gtggccgtga ttttgcacac aggtccggca
    10321 ggcctggctc gcgcgttgcg cacgcctccg gctggccctt ggacgggggc cgcttcacgc
    10381 gcgcgcctgc cgtccatgtg gacaaaacca cgggagagat ttgggaggca tgaggatcgt
    10441 gatcttgccg cttgtcgggg tggggccccc gccccacccc ttcgcgcggc gacgacagcg
    10501 atgccacatt cctccgcaca aatcgcaatc gctcgaagtt tcgtatatgg tatgaacgaa
    10561 agtaagtgga gggcgtggcc gtcatgagga cggcaaccgc catgtgatat tgggcgatgc
    10621 cggtgcggtt ggtgtctgaa caacccccga aggctgccga gagggcatcg ttccagccgc
    10681 cccagaattt tcgtctgacg gcttttggcg attgggtcgc cacgttcctc gccgcaacac
    10741 gaaattcttg cgggcgggcg tcccaggggc ctcagcgttg atccggctcg cgatgtagaa
    10801 gccgaccgcg cccagcactt cggcgagcaa caccggcacc agcaacgctg tgaactgcac
    10861 cgcctgcggg cgggcgccgg tgatcgcggc gaacagcgtc acatggggct cggcatcggt
    10921 cggaggtgag gcggcgagtt gcgcgttgag gcgggcgatg gcagtcgtgg cgatcgccac
    10981 gcggtcgcag aaggtgcggg catccttgga cttcggcaaa gcgcatgcgc tggtggcggc
    11041 ataggtcggg ctcgcatgga gaagggcgag ctctgcagtg tggcgcgcgt gttcggtctc
    11101 ggtacgggcg cgggtctggg ttgcgccgac acgcacggcc ttggtgtcat cgcgggtgat
    11161 cgcgatttgc tggatcgcat tccacacgct gttggcaaag aggagcggga acaggagcgt
    11221 gcacaaggcg gcgaccaggt aggcacgttg gcgccaggca cgagatgcag cgacaagcac
    11281 agcgcacttg ccgatgtcga gggcggcata gagcgccgcg aacagtacgg cgtattcaaa
    11341 caccttcgtc gaggtgaagc cgccgtgcgc gttgaggatg atcgtggcca gcgcggcgat
    11401 cagagaggcg atgcggagcg caatcatggg cggcctcgcg gattgaatag cggtgcgaag
    11461 ggaaactgga ccttggtagt tcggccggta tccgactggt cgacgatggc ctgtccggtg
    11521 gtgagcttcc ttggatcgtt ggtcggggtc agctcgccaa agagggacac cgcatggctt
    11581 tgctcgggaa gacggaagac gatgcgtgtg ctgcagttcg tgcgcagcgt cacggggatc
    11641 tgatcggcaa ccggggactg cgtgatgagc atcatgcgga tgccggcgga gcggccgcgc
    11701 atggcgatct tgagcatgtt gtcgaggaag gcctcgtgct cggccctctg cgctttgggc
    11761 ggcttggcga tataggtcgg cagctcgtcg atgacgagag cgatgtaatc gcgcgtggct
    11821 ttgtccacgc ccagccggcg ttgggcaata ttgcgctcct tcatgagcac ggtgaggcgt
    11881 tgggcgagcg cccagacatc gggctcgtcg tgcagcacgc ggattttcgg atgaagaccg
    11941 gcgtacctca taaaagcaac gccatccttg ccgtcgacga gatggacttc ggcaaagagg
    12001 tcgatgttgg cgaagatgga gcgcagcaga atgtggacgg cgttggtctt tccggcgccg
    12061 gcggcgccgg tgatgtaggt gcccgaggac agctctgcaa aggggatgtg cacgggcatg
    12121 cgctgcgtca catcgatgcc ggcgaagagg gcgccggcgg tgagatgggc cgagcggtcg
    12181 aaagggatag atggggggag ttgcgattgg cgcgtgatga cgaggatcga cgcactgtgg
    12241 tcggtgatgg tccattgaaa cgggaacaca ccggcgttga gccaggcgtt gatggccggg
    12301 aggtgcgcgc gccagaggtc ggcgtctacg ggtccgccgg tgtgcaccat gaccagcgtc
    12361 gttgcggtgg agtgttcaag gtccagctcg ggaaagcggt cgccctggtt gagcttgagg
    12421 aagggcccga gcagataggg gcgggccttc atcggcagcc gatggaaatc tacttccaac
    12481 ggcttgggag caagggcgcc ggcaaggcct gatttgaagg gatcgatcat ctcgcggagc
    12541 atgcgacggt aggcgtcggt gatgcgctgg cggacgcgcg gatcgggatt gcgctgattg
    12601 aactcggcaa ggccttgcag gcgctcgggg tgccgcagga gatcgagacg cgcattgagg
    12661 acgtcgcagg tctgctggtc gatctcttgc gcgtgtttga ggcgatggtt gagatcggcg
    12721 agcgtagcgg cgcgctggcg gcgggcggtg acgatgacgc gggcgcgctg catgtcgtgg
    12781 tcggtgatgg tcatggccgc accttcggga gatcgttgta attggcaagg cgacggcgca
    12841 cctcggcgat gtcgcgatcg agcgcgatgt tggcctcaac gagccacgag cagaacttgt
    12901 cccacgtcgg atcggcgacg ccgcgttggt aacggtccca aagtctcaac acctcagcat
    12961 cgaggggatc gaccgaattg gccggcaccg ggtggaatgt cggcgccgcc ttgagggcaa
    13021 gggatggcgc caccggcgcg ggcgtggccg gtggggccgt aacggaaccg aacaggcggt
    13081 cgaggaagcg catgggtcac gccttgcggc tggtctcgat ggtgaaggtg agaccggggt
    13141 ccttggccgg cttgatctcg agaacgggct tgaacacgta gccgggcggc aagggcggcg
    13201 gggtggtgta ggtccgcacg gtctgcgggt agcgtctggg cgggggcgcc atgacgggtg
    13261 gtgacggcgg gatgggatcg aagatcggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnatgg aaaagcgggg tgtgaggccg cgccggatga
    13441 tctgctcggc cgcgtactgg acagcggcag ccaggccgac atcgagactt tcgagataac
    13501 tcttgccatt ctccagggcg agcatgacgg tgtcaccggc cccaaaaggg ggcagtgaca
    13561 gcgtgtactt ctcgatgcga ccgtcgacat ggacggcggt gacaatgacc ttggcgacga
    13621 gaccttcgct cacacattgc tggatggcac ggatctgccc gtcgtccttc atgccggtgt
    13681 tcttgaggaa ctcgccggca aaccccgcat agagaagggg cttgggcacg ggcgtcagct
    13741 tcaaagcgat cttgggaggg aggagtgagg tcatggtcgt ggtcctcggc catttgaaag
    13801 gggaagggac nnnnnnnnnn agcgccggat cagcgacgcc accagcggcg tttgccgaga
    13861 tagcttcgaa cgcggctggt ccgaatttgc ttggcggcct cccaggcgat cgcggacacg
    13921 agaggcaggc gatgcccatg tgcagcatcg acggcagcct tgtcgggcat ccccgtgtcg
    13981 atcagcttct tggcatgcag tgcgcgcatg cgatgatgga gcgcgttcca gaatgcgacc
    14041 ggctgcgcgg cgtgcttacg aacaagcgcg gcaacgagct cgcgctcggg tgtagtcttc
    14101 gggtcgagag ccgaccatgc gatgttggcc ttgtcctccg cactcggaac atgatcatgc
    14161 cagatcgcgc gaccggggcg atggagactt tcccgcatgc cggtcgcatc gttgaccgtg
    14221 cagataaagc cgatgttcga tccatcgaca ttgcggtaga ggtggcgatt gtcagccaac
    14281 gccatgaact cgttgaccag gagctggctg ttgacggtcg agccttggtt ctcgccgacg
    14341 ttggcggtcg acaggtcgaa gtcgtcgatg atgacgacga cgcggcagcg atgcgcagcc
    14401 gaccatgcca ccagttcagt aaggacgtcg tgcagggcct ggaccgaggc cccttcgacg
    14461 tcgccggcga agaggcccgg cgagagcacg gcggtatgga agccagcgtt caggcacgca
    14521 acgaggcagc ccagcgactt gccttctccc ggacggccct ggacccagag catccgcgcc
    14581 gcatcgttgc gggtcgcctc ggcgatctgg ttgacgagat gctcgcgcac ggtcagcgac
    14641 agggcgcgga actcgaagcg ctggcggacg atcggatcgc cgggtttgcg gatgaggtgg
    14701 gcgtattgcc aggggccgtg cggcggggtg gcgccgttga cggtatgacc ggtggcgggc
    14761 gtgccggcaa ggttcgggaa atgagtgctc atcgagggct ccttattcga ggccggacat
    14821 gaaggtgtcg ggatcggcgg cagtcacgat ggtgccgggg tccttgtgcg cattgatcag
    14881 tgcgagcagg gtcgaccagg cggcgcgctc ggcggcggtg tccaggggcg gcggactggc
    14941 gtcgacggcg aggtccgccg gctgatcggg atagggatcg tcgctggcct gcgaggatcg
    15001 ggccggcgac cccgggggcg gcgcgaacag ttcggcagct gccggatcgg cgttggccgc
    15061 cggctgccca ggaaatcccc cggcaatgcg gacgatctcg tccagctcat cgagggcgtc
    15121 acgcacgacg tccttctcgt ccttgagcgc cggctggcgc agggtctcgg cggacttggg
    15181 ctcgatcacg gggccggtcg gccaactgaa tttcggcgtc gtcatggatc atgctccgat
    15241 gtcagaggaa gagtccggcg cgagatcgct cccgcgccgg aggtgagctg atgtcaatcg
    15301 aaagtgggcg ccggcaggat cggcgtgttg ggcaccagcg tgaacgagag acgaccggtc
    15361 tttttcgaga ccaccgctgt ggtctggaag ccgaagacct tctgcggggt aagggctgac
    15421 gcgttgaaat ccggtccgat cgagatcgac tcgcgggtga ccgtcccgtc accgtgtgcg
    15481 agcagcacct tcagcgagcg cagcggacgg acgacgttgg tcttggggtc ggtgtactgg
    15541 aacacatcct ctttctcgaa cgtcgcgaac aggacgatgc cttgcagtgt cgtggccatg
    15601 tgaagctcct ctattggccg tcgaagtctc cggcggaagc cggatggatg gcgggcgctg
    15661 gcccggatca ggatggattg cgtgggtttg ttttcaggcg ttgttgcgcg tccttgaaac
    15721 agcggtcgaa actgacaagc tgctggcctt tgagcaaagt gtgcagcgcg agcacgatgg
    15781 tcggatcggc ggtgatgccg ttatcgtcgt gcgcgaaatg tgccggcgag atcgcatact
    15841 tcgcgagctc agccgcgctt cggcggaccg atgcgggatc ggtttcgccg gagggaccgc
    15901 gtgcagcgcg gatatccacc acgggcttgc ggtccagtcg cgcagcggtc tgccaccatt
    15961 gagcccacgt tgtctgatgc acgcggggta cgccgccctt gaagtagccg ccaggcccac
    16021 catcgacgat gacgaggcca tgcgcgtgga tgtgcagtgt cggcgcgtcc ggtgtacctc
    16081 cgaccgtcac ctcgatggag atgaagatgc cgcgcgtgtt ggcgatcaca tccggtatgg
    16141 cgcggaaacg cttgagggca gccttgagcg gctttagcga ggcggatacg gcgggttgtg
    16201 ccggcagcgt cagggtcaag aagagcgcgc gagcgtccgg cctggccgcg aacacctcat
    16261 caatgagagc ttgcactttg cgacgcatgg tggcggcgct ccaagcggcc gagatgggac
    16321 agaggcgact gcggcaatca cgttgattgc gggtgattat ctccgtcgct tccccgcgag
    16381 gtgtgcgcac acggacgtgg aacgaggtcg cgcaggcctc gatgcgccgc gcgagacggg
    16441 cgacagttcg ccccttcgcc ggtccgatca tgtcggcggc ggcatccaga gcacgggaga
    16501 tggcgaacgc gctctcctta cggagactga tgcgggcgac ccgcgcggcg accggcgaag
    16561 cgaaagtcac aacgcacctc cgcgatcgga ggtcgacgtg cggcgagaag cagcgacata
    16621 ggctctcagc tcgccggcga gataggtcac ccggcttctg agacccgggc ccttctgatg
    16681 agccgggccg atcccctgac tgcgccaccg ctcgagcgtc tgcgtgctga cacgcagaac
    16741 gcgcgcggcc tcaagcgtgg tgagctgcac cgtatcgtcg gcgggcaggc cgaggaattc
    16801 aggcagggcg gtgtcgttcg cggacatgag ggtcctcgtt ggatgattcg ggtcttgacg
    16861 annnnnnnnn nnnnnnnnca actgaacagg cccgccgcag tgcgctgatt tacgggcggt
    16921 gaagctggat actcgaggaa gctcgcgacg gcgtacatgt gctcattcgg cgcctcgttt
    16981 tagtcagttg cctcgatacc aggttgacaa gagcggccga tgcctcgaac tcggtaatgc
    17041 gacgctccag gtcagtttgt tctcgttcca aagcatcaat gattttgccc gcgtgggaca
    17101 cggcgtcgtc agagttacgc aacgtgatac ctaggcgctg agccagtatt cgaagcagtt
    17161 caccgacgac gatgtcactc ggacgcaatc gggtgctggt ctccgctact ccgcgcttgg
    17221 tcaaatgggt agcgtcaata gacatgaggc tcctcgttgg atgctgcgtg ttgttgtcgg
    17281 aaaccggcgc tgaagcgtcg gggtggaaca ttagctacgc ttcatatttg acgcggcagt
    17341 cgccacagat gaggttgagg ctcggttttc cccaggcgtt tgatccgcac tgcggacaag
    17401 tgaatttggt gcgcttgccg gcgctgggtg ctggcagact tccagggttt tccgcaccgc
    17461 cttccgagac acgcgacatt tcggtgaggc ggtcatacca agcgatacgg aactgatcgg
    17521 acaggagctt ccggcacgcg atatcgaagg ctccaccggc gacgatgata tgcgtcatgc
    17581 gctggccatg cagtcgacct tcaggtgtcc cgtcgtgcgt gggacgcaag ccaatgtcct
    17641 gcatccactg cgaccactgc ttgttgtggt agccaacgcg tgagggtttt ccctccggat
    17701 cgtgatgttg ccgcaaatgc accatttcat gggcgagggt cgacagtgtt tcttcgatgg
    17761 agcgccgccg aaagtggcgg gggttcatgg cgatttcatc gaccgtgaga ttggaccgct
    17821 cgcggtggcc aaaccgatcg ccatggaaat agccaagaac gcgttcggca ttgcgttgca
    17881 gggtgatgat gcaggccggc agcatgccgc caaacagctc ccgattgaaa tgatcgtagg
    17941 cgacgatcag cggcccgtaa gcctggacgg tcggatgggc cgaggcagat tgatcccgtt
    18001 gcgcctggtc atttgtagtg tgcacgacat cggcgctgat ctcgggttcg atctcggaca
    18061 gcttgtgcgc gagggcgatc tcgttgaact tgcgcgtcat ggcgtcgact ccagtccctt
    18121 gttttgcgca ccacagaggc ggtgcttgtg tgaatgcatc tgcgagaggc cgggcgaggg
    18181 agcggccaac ctcactgcag gggaaccggg cctaggccgc gagggctgcc tcgtcggatg
    18241 tttccggctt gatctcggcc cctcgtccgg gcaatgctgg cggctggttg tcgagcaagt
    18301 aagcgagcca tttggcgatg acccgctcct cgcgcggggt gcggtacccg ctgctgaggg
    18361 cctggtagag ggccttgatg gcctcggggt tacggggatg tctcatgctg cgtgctcctg
    18421 cgtcccgccg cttagcatcg cgccggttac gggctgcgcg ggccggattg tgctgctgtt
    18481 gcatcgagtt gacccctcta gtgctctgct gccggttgtc ggcggctcat gagggaaaat
    18541 ctggcagagt cggtcgaagg acaaaatcag ggttcgtccc tcagatttcg atttgtgagg
    18601 gagagaagtg aggtggcgta gtgagtgagc aatcatccta tcgccacagg agactcgcat
    18661 gtgggcgtta cgacaatatg agaacagagt tcttcaagca caggaagaca ttgccgccga
    18721 aactggagca ttgaagaaag aagcaactac gccttggtca aacagccgac ttagacgacg
    18781 ctcagatcgg gttttcgggc gcgagtctgg tcgactgcca agtgagcgat ttaccggagc
    18841 agatcatgag aagatgaagg actctttgcg catgctggcg gatggcgtcg agaaacaatc
    18901 ccggcttgag gaccatcttg aagccattga gcagctgttc gttgagtatg agcagaaggt
    18961 cattcttatt caacagcggg tgcccaagtt gttcaaggat aacatgcgca ggcgcggcat
    19021 tatatcaata aaagacctgc tgaccccaga aggtttcaac aaagggatat atgcgccaat
    19081 atatgaggcg atgattgcag gtcgcttcgg tgaatgtatt gacaatcttt ctcaatcgtt
    19141 gagtgcgtta gcgaatgatg ccacattgaa atcgtcgaag ttctcagggt caatacttgc
    19201 cgccacacta agcatttcgt tttactgcct gcatcgaatg ggccatcctg gacaagctga
    19261 actcttggaa tatctggtcc gcatgcagag ccaagatatt gccgaagaag atcgcaagtc
    19321 gttgagaatg gcattgttgg cttatgcttc ttatgaggac ccgcattcta agagcgcacg
    19381 tcaggcccgg gaactcgcgg gaccttccgg cttaaaggcc cggtgggagc ccggaattgc
    19441 agggtgtgaa ctgagactgg ctttggacta cggtttagcg aagaggctgg ctccagacga
    19501 acctatgagg gatttggaaa gagctaacat tcgaagtgtg gtcaaggctc acgaagccct
    19561 tggtgacaac gtcctctcgg ggtcatactg gaagccgaaa acagcactcg tctcagccta
    19621 tgcacaccac ggagggctcg atgacgtcat cgcgtgcgcg tatgcaataa gattggaaat
    19681 gctgaaaaag aatatcgaca tgccgcacgt ggaaatcagg cttctggttg atgaagcaag
    19741 ggtgctttgg gagaagtcat cgtcgaagaa atgtcatctt gcagtcatgg cagccgagaa
    19801 agttatggca gcaaggcgga tatcgggacg tacaggcatc ccgttggttc acggagcaag
    19861 aacggcgtat gtgcaatccg ttattgacga aattcaactg gacgagcata ctcacggcag
    19921 taacaggagg gtggcatgag tggagtccaa caaggatatt cgcagacggt acaacgatat
    19981 ttgtagacgg atcggggacg cacgcactgc tcaggagaag ttttacgcat ttcgagcgct
    20041 atatagatac tatccaaagt tatacaagct tagcggcaag agccatcctt ttcacaattc
    20101 tcttttgcat gatccaatga tggatcttgt gagcagtaca tgcctcagtc ataaatggtg
    20161 ggaggttaaa tcaagtagca tcaatatgcg ctggggcgca gttctggacg caaaagagct
    20221 tggcacgaga gtagcgcctc ttccgtgagt tcgtgggcgc gtcgacgttt atgaaaattg
    20281 cttccaccca actgatgcga aagaggcgcg aagcgcccat cagataattc ctttgaattg
    20341 cacgcctcat cttatcgcgg agtgcgaatt cagaatctca ctttgcgact tccacccaca
    20401 tcagcaacgg ctgcactagt gaataatacc ccgaagtcgg attttaatag ccaaagcggt
    20461 tttggttctg acgccgtcgc cggccggagc cacttactca cagccctttt gtcaaaacgt
    20521 ctttttagat atgttttaat agttttagta gttttagcaa attcaactcg gccggttgcc
    20581 aaaaatgcca cccgaaattc agcgacttgg aagcaaaccg atagtcgatg tacggcatcg
    20641 agatagatga tgtacggcat gtcatagatg atgtacggga tgtcatagtc gatgtacggc
    20701 tttggataga tgatgtacgg cggcctgcca gtattatcca caaggcgggc acgataggtg
    20761 atgtacgcct ttgacaaaat ggactgacaa aagcgccgcc gaaatcggcc caacttagtc
    20821 gatgtacggt tctgacaaaa ttgcttgtca aattggcgac ggcgtcaggt ggagcacgct
    20881 ggcacggtat tgcatgacga gcttgggctc ggtgaggccg tcggccaatt ccagatcgta
    20941 gtcggggaac tggaacagct tggtgctggc gacgaagcgg cggagcatct gggcgaactg
    21001 cggcttgggc tgggaggagc cgctgcgctt gtgcaattcg gtgaggccat actcggcgcg
    21061 ggtcttgccg gcggctttgc gcgcgaggcg atagatgacc ttgccgatgg actggctgat
    21121 gaggaaatag tcggggttga tggtgaggat gtgcggggtg gcgttggtgt tgacgacgcc
    21181 gtcatagacc caatccggca ccgcaatctc gacctgatcg acgtggccgg aggtggtctt
    21241 gctgatcacc ttgaaattct ggatgagcgg catgttgagc acctcacggc ggcggccgtt
    21301 ggagaggttg atgatcttga tgcgagtgcc ggcgaggcgg tcgagcgcgg attccacgtc
    21361 gcggtattgc ttccccccgg aattccggcg gcagaacttg aggatgtggg cgacgttggg
    21421 ccggtagatg cgggccggca gatggggccg ctggcccttg ttaatcgcca ggtggtagcg
    21481 gcgggcttcc tcggccatgt gcgagaccat gtgcaggaag atgtcgtagt cgaatgcggt
    21541 ggcgagcccg gcaccggcgc tgccgtcgat ggtgatggtg cagtccttga gctcgtaggt
    21601 gatgacgcca tcgcgacggc ccttggcgag ggagaacggc gcgatgtcca tcagattcat
    21661 gtcgtctttg atgggggcgt cgtaaatgtg cggcacgaag aagctcagtt ggccgtcgtc
    21721 ggccggtggc gtgcgcagga ccggcgcagg cagatcggtg gcgagggcgc cgagaatgtc
    21781 ctggcggcga tcggcatgct tgatggcgtt accaagggcg cgcagggcgc gattgannnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgtagaa gcggcgcaaa cgggccaggc
    21901 cttcgggagt gagcggggtc ggcggacggt tggtggcttc ggcaaagcgt cgggccaggt
    21961 cgtggaggta atcgttcttc tggtgcaacg agagcgccgc aaactcccct tgggagatga
    22021 gatcgagttt gggtgcggtc tgcgcggtca cggctcggtg cgatcactgc ggcgggcatc
    22081 ctcaacgagg tcggcggggc ggatggcgat gccgaactga tcgagggctt tgaggacaat
    22141 gaagcggacg gagctgtcgg cctcgaatgc cttggccttg agggcagcca cggcgtagtc
    22201 gggcacgtgg atgttgatgg ccttggtgag gctgcggggc tcggccgggg gcgcgtcgcg
    22261 tgtcaccggc gggaccaggg tcgggacgcc gagctgccgg ttgagcgtgt cgagcgcggt
    22321 gtcgggaaca tcgaggggcg ctccgatggg tcggaaaaca gggctgttca agcggcgtcc
    22381 tttcgattga tgggttgctg ggtcgggagc aggagggcga tctctgcgag ccactggtcg
    22441 atctcggcaa cggctttggc ggtggtcgag cgggtgcggt tggattgatg gggcggcgat
    22501 cccgagagac cgatttcctg ataggcggcg cggtgcgtca gcatcgtgta gaagcgcggc
    22561 agggccaggc ggtcgacttc gcggatggca tgggattgag cgtgcgaggc gagcggctgg
    22621 acctgggtga gcaacaggcg gaacagcggc tcgcggttga aggcggcaaa gacgctccgg
    22681 agatgggtgg cgatgcggtt agcctcgaac agatccgcct cggtcggcgc ggccggaatg
    22741 atggttagat gggcgcgggc catggcgacg gtgatggcgc gctcataggc gccggccacg
    22801 tcgatgagga cgaagtcgag ggatttcttg gccagcatgt caaggtgctc ggtgagcgcc
    22861 agcgggtcgg gcgtcgagac ggagaaaccc ttcgagcgcg agagccagcg agagacggtg
    22921 tggttgttgt cgaggtcgac gatatggacg gtgtggccac gggcaacgag tgcgccggcg
    22981 agacaggcgc aggcggtgga cttgcctacg ccgcccttgg atgaggcgaa tgtaatgtgc
    23041 aaatgtaaat tcctttaatt gtggaaatat ggaattgacg atttatatat catggaagag
    23101 ttggccgcaa tgggccgcga caatggggac gggtgggttg ttgcaaaaat gcaaatgcgc
    23161 agatgttgaa aaatacaatt caacaaatat aggaatgagc aagaatggga gtgtggattt
    23221 atggatttga agaagtatcg tcaaatgaca gccgcgaaat atccatcgcc ccgtgcagaa
    23281 agcggatgat gcgggtttcg gttggggtga cgcggaaata aatgcagtag ttgcccaaga
    23341 cgtgcaggcg gaggccgggg ctcagccact cgcgggaggc gccggaatgg ccgatccaag
    23401 ccaagcggga tagttcggcg tcgatgtcgt cggcgaagcg gagggcgata tcgagcccgg
    23461 cttcgcgggc gatgtaggca aggcaggcgt cgatgtcggc gcgggccggt gcggtcacgt
    23521 cgagtgattt cggaccaagg ggagcgggcg agcgagacat acaagcaggc tacgcggagt
    23581 cgccggttcg agccagcgct gtgccgcgtg tttcagcgct cgggggtatg gtccttgagc
    23641 agcttggctt tgagattggc ggcaagctgg cccggctcgg tgtaacgggt cacgtcgccg
    23701 cgtgcgtagg cggcatcacc ttcggcgatg gcgttgcgaa gctcggcgag cttcaagtgc
    23761 tcgcgttcga gaaggcgcaa gcccgcgcgg atgacttcgc tcgcgttacc gtactcgccg
    23821 gaatcgagct gggcggcgat gaaggcgtca tccttggcgg tcagggaaat cgtctttcgg
    23881 tgttgcatag gttcagtata gggcgatgca cggctccgtg caatgggtat gataaccact
    23941 acgccgcttt ggtggggttg ccgttgaggg cggcggccag agtctcgccg gtctgctggg
    24001 caagcaggcg caacggatcg tcggccagat gggcgtagcg cgctgttgtt cttggcgtca
    24061 tgtggccaag ctggcggccg atcacggcga gggacgctcc ggaaatcagc gccgtgctgg
    24121 cccacgtatg cctgagatcg tgcaggcgnn nnnnnnnnat tgtcgctgag ttctcacctt
    24181 gattgtcgcc gcccaccggt gatgttggcg cgctcacaga gggtgtccca ggcgtaatgg
    24241 agattggcga ggtgggtggc gtggatcttc ccgacgatca catagggatt gcccgcgagg
    24301 cgcgggacct cgcgcaagac ggcgatggcc gcatcgttga gcgtgatggt cttctggccg
    24361 gtcttgctgt cgggcaggaa caggaggccc cggtccaggt tgatgaagga ccattgcagg
    24421 gtgagtattt cagagaggcg ggccccggtc aggatcaaga gccggatagc ggcggtgcca
    24481 tgagggttga tggagccgtc gcgctcgcaa tcggcaagcg cggcgccgat ccgggagacc
    24541 tcgtcaaggg agaggtagcg ttcgcgcctc acctccttgt agcgcttgac gcgcgtgaca
    24601 gggttgtcac cctcacgcaa gccctcgtcc ttggaccagg tcatgagctt ggacaacacg
    24661 gtgagggcga aattagctga gcgcggcgtg tcactccagg cgaggcttgc cgtgctgaca
    24721 tcggcgcggg tgatggcggt gaggggttta gcgccgaagg cggggttgag atagaggcgg
    24781 atcagccgct cgtattcgac cctggtgcgg ggccggagca tcgggccgtg cttagccagg
    24841 aacaaaaccg cgacattctc gaaggtgaga gggcctggct gctggtcggg cttggacttg
    24901 gtgggcttct cggcgatcgc gggattgccc atgaccttga cggcctgacg gcgggcggtg
    24961 tccggcgtcc atgtgttgcc gtcgggcgtc gccataccgt gttggccgat gacgaagaag
    25021 cgctggcgac cgtggatacg cgtccgaagg atgtatgtca cggcgtcgga gcgttggcgg
    25081 cggataccga atcctcgcag ttgggtatcc cagatgaagg tgttgggcgt gaccgagttg
    25141 atgacggtga gtgtgatctt cttacggatc ggtttgggca tgggcagatc cgtccgtgag
    25201 tgagtggcac acgttggaac gagggcttcc aacaagctga tgacatagcc tagtgagcat
    25261 ctagtgagca agatattcaa aactacacgt caaagttgag ggttcttgag tcggtttatc
    25321 ttgtaaaatc aatagtacag acagttgatg attgttactt aaggttgtat taaacagcct
    25381 tccaagctga tgacgagggt tcgattccct tcacccgctc caataactcg atgattttat
    25441 tgatgatatt tcaaacgctt ttcgatctgc ccacaggctg atgcctttgc cgtgccacgt
    25501 cacgcttgct attgatattg ctggactttc cagagcaaac gccgtcagaa agtgccacgt
    25561 ctgtgccatg ctccctgtcc acagtacggg cttgcacgac gccatcgaga agttgaagca
    25621 ggagcggcgt gactgatcgg cacgactacc gacgcactcc gtgtcgccta atcggctccc
    25681 attgcgagcc ggtagccgat gccggcctcg gtctggatga ggtcgggatc gttcggatcg
    25741 cctttcagct tctgccgcag ctgcccgatg aagacgcgca ggtactgcag ggcctcggta
    25801 tgggccgggc cccacacggc ggtcaggatc tgccggtgcg tgacgacgcg gccggcattg
    25861 cgggcgagga tcgcgagcag ctcgaactcc ttcggtgtca gcttgatcgg agtgcccgcg
    25921 cgcgagacga cgtgagcagc gacgtcgacg accaggtcgc cgcttgcgat cctcgagacc
    25981 tcgcccatgc tctgcgcggc atggcggagc gcggtcctga ggcgcgccat cagctcgccg
    26041 atcccgaacg gcttgttgac gtaatcgtcg gcgccgagat cgagcgcggc gatcttctcg
    26101 gcctcgcggt cgcgcgcgga aaggacgacg atcggcacct tcgaccaggt gcgcagctgg
    26161 gcgatcacat ctttgccgtc catgtcggga aggccgaggt cgatcaggat gacatcaggg
    26221 gctcgggtcg ccgcagcctt gagtgcctcg gcaccattga cagcggcgat gacctcgtag
    26281 cctgcagcgc tgaggcttgg cttcagaaac cgctggatct gaggctcgtc gtcgacgacc
    26341 agcacccgat gcgtcgtcat cattggcctg ctgcctcttc agtcccccga ttcgccgcaa
    26401 ccggaagcat aatcgtaatc ctcgtcccgc gattgccggc gacggggctc tcggccctga
    26461 tgatgccccc catacctttg acgatgccag cacagatcga caggccgaga ccggtgccag
    26521 gggcacgacc atcactgcct gctacgcgat agaatttgtc aaacacccgt tccaactctt
    26581 ccggcgggat gccgatgccg cgatcggtaa ccgtgagagt gatcccggcg gattcggccg
    26641 cgaccttgag ctccgtctgg gagccttggt cagtatactt gtgcgcattg tcgagaagat
    26701 tgaacagaac ctgctccaga agtgccgcgt cgcctctgat cagaggtatt ttttctgaaa
    26761 ccgcggtcgc aattttccgt cttggaaacg ccttgcgcgc tcgttcgacg gcgcttgcaa
    26821 cgacgtcgcc gacatcgacc cagtcccgac ggatgtcgat ggcgccggcc tcgagcctgg
    26881 tcatgtccag caggtttgag acgaagcggg agagccggcc ggcttcctct tcgatggtcg
    26941 caagtaggtc accccgatcc aactgcgaca tgcgatcacc aagcgtgcgt aggctggtca
    27001 ccgagccgac gatggaagcc aatggtgtgc gcaggtcgtg cgagatcgag gatagcaacg
    27061 cggcccttag gcgttctccc gcggcggcgg tctcagctct tgtcgcttgc tcgacgagag
    27121 cgatcctctc gatggcgaca gcggcttgct cgatgaacga ctggatggtc gcaatcacgg
    27181 cgtcgctcag tgcatcgtcg gcgtctcccg cgtcgatccc gaccgtaccg atcgaacccc
    27241 tggacccctg catgggccgg aattgaaagc gcgccgacgg cagcgtatct gtgctccgcc
    27301 cggcaggttc ctgcttttca tgtgcccacc gcgccgccgc ccaatccgat gttccgagct
    27361 gatcgtcggg tggccagcta ttctcaagtg tcagtccgtc actgcgatcg agaagcacga
    27421 ctgacttgcc cttagccaca gccgcaacct ggctggccag cagccagagc acatcgtcga
    27481 gcttcgatac acccgagagc ttgcgtgaga aatcgaatag cgcctgggtg gcctcggcgc
    27541 gctcgcgggt ggcggtcgcc tgctcactca gccggccggc aagagagccg gtcacaacgg
    27601 cgacggcaag gaacatgaac aatgacagca gctcgtatgg ctgggtgatt gtgaaagtgt
    27661 atcgtgggtc gatgaaaaag aaattgtagg cgaggaatga aagcaccgcc gcggcgattg
    27721 ccgaggtcat gccaaagcgc atgccgcaga gcagaacggc cagcaggaac accatcgaca
    27781 ggttcggcaa tcgcgttatc tgctcgagcg caatccccgc gagcacggct gctgtgacag
    27841 cgcctccggc aatggccagt gctgccgcca aggttccagc aggtggcaag gcactccttg
    27901 gttgggcgga ggccccagct tttgacggct tctctgactc cggtgcgacg atgagaaccg
    27961 aaaggccgtc ggcgcgccga acgagatcgc gcgacaggga tcgcccaaga agccgcgcga
    28021 ggggaccagc cttcgagcgc ccgatgataa tctgggtcac gttgttgcgc ttggcatagg
    28081 cgaggatctc gcccgccagg tccgtcgcgc tgaggcgcac ggtggtcccc ccgaggcgnn
    28141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnn atgccctgct ggcgcaattg ctgcagcatc tgctcgtcga
    28381 cgcggtcagc cgtgcggcgc aacgcaagct cacgcaaagc tgtcaagttg ctgggcttga
    28441 agaactggtc gatggcgcgg cgtgcgttgt cgggcaggta gaccttgccc tccttcaagc
    28501 gttggacgag ctcctccggc ggtagatcga cgacgacgat ctcgtcggct ttttccagga
    28561 ccttgtctgg tacggtctcc tgcacgcgga cgccggtgat cttctggacg acgtcctgca
    28621 ggctttcgag atgctggata ttgagcgtgg tccatacgtc gatgttgttg cgaagagcct
    28681 cctccacatc ctgatagcgt ttggcgtgga tcaggcccgg cgcgttcgta nnnnnnnnnn
    28741 nnnnnnagga gcagcttcgg cttgcgggca atcgcctcct cgacgtcgaa ctccatcagc
    28801 gtactgcgac gatactgcac gggcttgcgg ggcagcacct cgaggccttc gagcagagcg
    28861 gccgtctcgg aacgcccgtg cgtctcgacg acgccgacga ggacatccac gccttcggcc
    28921 ttctgcgcgc gtgccgccga gagcatggcg taggtcttgc cgacacccgg cgccatgccg
    28981 aggaacacct tgagcttgcc gcggccttcc tttgccgcaa gctcgagcag cgccttgggg
    29041 gaagggcgcc gctcgttggg agatttggtg ttgggatcgg ccatgctcgc ctatccggcg
    29101 ccgcctggca ggctggcgtc aagagtgagg ttcaagcgca gcacgttgat tctgggctcg
    29161 ccgaacagtc cgagcaatgg gcgctccgtc gaattctcga caattgcgcg gatctgggcc
    29221 gcggtcacgt tccgggcggc ggcgacgcgg gcgatctgca agccggcgaa agcgggtgag
    29281 atgtgcggat cgagcccgct ggccgaggtt gtcacggcat cggccgggat gattgaagcg
    29341 ccagccttgc gcagggttgc aacgtcgccc tcaacgcgct cgatcagttt cttcgacagc
    29401 ggtccgaggt tggagcccga ggatcccgtg gcgtcgtagc ccttctcgcc ggccgccgag
    29461 ggtctcggcc tgaagtaacg atccgatgtg aaggactgag cgatcaagtc ggagccgacg
    29521 atggtcccgc ctcgggtgat gaccgaaccg ctggccgtcg ccgggatcag ggcttgagcg
    29581 aggacagtca tcgcgagggg ataggccacg ccggtcacta gcgtgaagag agcgagcatg
    29641 acgagcgcag gacggagaag cgtgagcatg gaaatctcct tcaggcgagc cgtagggcgg
    29701 aaacgaccag gtcgatgacc ttgatcccga cgaacggcac gatcaggcct cccagcccgt
    29761 agacgaggag gttgcgacgc agcaatgcgg cagcgccgat cggacgatag gcgatgcctt
    29821 tcagcgcgag cggaatcagc gcgacgatga cgagcgcgtt gaagatcacc gccgacagga
    29881 tggcgctttc gggcgttgcg agccccatga tgttgagcgc gccgagctcc ggataggtta
    29941 cgaggaacag cgcggggatg atggcgaaat acttggccac gtcgttggcg atcgagaacg
    30001 tcgtcagcga gccgcgcgtc atcaggagct gcttgccgat ctccgtcacc tcgatgagct
    30061 tcgtcggatt gctgtcgaga tcgaccatgt tgccggcctc gcgcgcggcc tgggtgccgg
    30121 actgcatggc gacgccgacg tcggcctgag cgagagccgg cgcatcgttc gtgccatcgc
    30181 cgcacatggc gatcaagcgc cccttggcct gctcggcctt gatgtaggcg agcttgtcct
    30241 gcggcgtcgc ctcggcgatg tagtcgtcga cgccggcctc cgacgcgatg gcggccgccg
    30301 tgaccggatt gtcgccggtg accatcaccg tcttgattcc catcgcgcgc aatgccgtga
    30361 atcgctcctt gatgccgggc ttcacgacgt ccttgagatg gacgacgccg agaagccgcg
    30421 gtccgtcggc tacggcgagc ggcgtgccgc cggatcgcgc gatgctgtca acggcttccc
    30481 ggaataccgc cggcgcatcc tcgatggtcg ttccgatcga cttcagaacg gcgtcgacgg
    30541 cgcccttgcg tatgatcctg ccgtcgacgt cgatccccga gatgcgagtc tgggcggtaa
    30601 aggggatata ggtggctctg cccgagcgct cggactccat ttggccgtaa gtgcccttgg
    30661 caagggcaac gatcgagcgt ccttccggcg tctcatcgga aagcgaggag agaagcgcgg
    30721 ccgcagcgac gtcctgttcc gatgcgcccg gagcggggat gaaggaggtc gcgaggcggt
    30781 tgccgtaggt gatggtgccg gtcttgtcga gaagcagcgt gtcgacgtcg cccgccgcct
    30841 cgacggcccg gccggacgtt gccatcacgt tgaagcggat gagccggtcc atgccggcga
    30901 tgccgatcgc ggagaggagc ccgccgatcg tcgtcgggat gagggtgacg agcagcgcga
    30961 ccagaacggt gatagagagt gccgttccgg agtagcccgc gagcccccag agcgtgacga
    31021 cggcgatcag aaagatgagc gtcatgccgg aaagcaggat cgacagcgcc agctcgttgg
    31081 gcgtcttctg gcgttgcgcc ccctcgacga gcgcaatcat cttgtcgagg aacgacgagc
    31141 ctggctcggc cgtcacacgc accttgatcc agtccgagat taccaaggta ccgccggtga
    31201 cggccgagcg gtcaccaccg ctctcgcgaa tgacgggggc ggactcgcct gtaacagcgg
    31261 actcgttcac cgacgcaatg ccctcgatca cctcgccgtc cgctggaatg gtatcaccgg
    31321 cctcgaccag cacgacgtca cccacttcga gcagttcggc ggccttcggc tcccacagat
    31381 cgtccttggg gtcatccgga tgtaagaata gcttggcttg cgtgtcgctc cgggtcgacc
    31441 gcagtgcgtc ggtatgcgct ttgccgcgtc cttccgcgat ggcctcggcg aagttggcga
    31501 acagcactgt gaaccacagc caagcggcga tctggcctga gaagagaagc ccgccgcggc
    31561 ctgacatgag atcgcgcacg aacagcaacg tcacgagtag cgcgacgacg gccgtgacga
    31621 acatcacggg attgcgcacc agcttggcag gggccagctt ggtgaaggca tcgactgccg
    31681 attggcgcag gatgccagta tcgaggagcg ggatggcttt tgtgctcatg gggtggctcc
    31741 ttcagaacgt cttgccggcg tgcatcagga agtgctcgac gattgggccg agggcgagcg
    31801 ccgggaagta ttgcagcccg ccgagaatca ggatcacgcc gatcaagagc ccgatgaaca
    31861 gcggcccgtg cgtcggaaac gtgccggagg acgcgggcac cttcttcttg gcggcaagcg
    31921 caccggcaat ggccatcacc ggcacgacgt aggcgaagcg gccgagcagc atcgcgatgc
    31981 cgagggtggt gttgtaccag gacgtgttgg cggagaggcc cgcgaatgcc gagccattgt
    32041 tgccggcggc cgacgagtag gcatagagga tctcggagag cccgcgtggg ccggcgttgg
    32101 cgaggctctc tagcgccttc ggcagcatgg ccgacgccgc ggtgaaaccg aggatggcga
    32161 gcggcaggat tagcagcgct agcatggcga gcttcatctc gcgcgcctcg atcttcttgc
    32221 cgagatattc gggcgtgcgc ccgaccatca ggccggcgat gaacacggtc aggatcgcga
    32281 tgacgatcat gccgtagagg ccggagccga cgccgccggg cagaatctcg ccgagctgga
    32341 tgaggaacag cggcacgagg ccgccgagtg gcatcagcga gttgtgcatg gtgttgacgg
    32401 cgccgcacga gaggcccgtc gtcgccacga cgtagagcgc ggagagcgcc tgtccgaagc
    32461 ggacttcctt gccctccatg ttgccggccg tcatgtcgac gccggccttg gcgagcagcg
    32521 gattgccaga ggtctcggcc cagtagacca cggccgtggc cgtgatgagc aagccgtaca
    32581 tggcgacgag cagcgcgata ccttggcggc ggtcgcctac catgcgaccg aaagtgaatg
    32641 tcagcgcggt cgagatcagc agcatgctcc agattgagag cacgttcgac caggcatttg
    32701 ggttctcgaa agggtgcgcg gcgttggcgt tgaagaagcc gccgccattg gtgccgagct
    32761 gctttatggc ttcctgtgaa gcgaccgggc cgagcgcaat ggtctgcttc gcgccttcga
    32821 gcgttgtcac ttccacgctt ccggcgagcg tttgcggcac gccggccagt gcaaaggcaa
    32881 tggcgactac gatcgacagc ggcagcagta cgtaaagcgt ggcgcgggtc acgtcgaccc
    32941 agaagttgcc gagcgtctgt gctgagcttc gcgcgaacgc ccgcgtcaca gcgatggcca
    33001 gggcgatacc ggtggctgcc gacaagaagt tctgcaccgt gagcccggcc atctgcacga
    33061 gatggctcat ggtggtctcg ccgccgtagg cctgccagtt ggtgttggtc gtgaagctca
    33121 tcgccgtgtt gaaggcgaga tcggacggca cagcgccgaa gccctgcgga ttgagcggca
    33181 ggatcgcctg caatcgcatc agcgcgtaga gcaggaggaa accgagcgca ttgaacatga
    33241 gcatggcgag cgtgtatgcg agccacgatt gctccttctc cgccttgatg ccggcagcac
    33301 cgtacagcgc gttctcgatg ggcttgagta cgccggtaag gagtgtgcgc tcaccctgga
    33361 aaacctgcgc catgaagcgg ccaaggggcc acgcgcaggc gaggaccccg ccgagcacca
    33421 gcgcgatctg cagccaacca atgagtgtca tgggttcacc tcccgaggct cagaatttct
    33481 cgggccgaag cagggtgtag acgaggtagg cgccgagccc gacagcgacc agcagcccga
    33541 tcacggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33601 nnnccatcag cgcaaagccg ccgaggccga gtgcgagaaa gacgagatcg agcatggtgc
    33661 ttctccgcag atgcatcgca gaagcaaact cccatgacct cgcataagga ctcgattggg
    33721 aactgtggca gcaggcataa agacgatata aggatcaagc ggctcaggcc cggcaagccg
    33781 agctgcatga gtggaaagca cccaactgcg cattgatgtt ggaaaagtca gccttagggt
    33841 acagcgtcgg aggcgtgcag ctcgcaacgc agacaagcga cgcggtcagg agatgaaccg
    33901 ccccgggttt gccggaggct ccaactcttg agagattgga gccatgacgn nnnnnnnnnn
    33961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gcatcgcagc
    34021 gaatacacgt cgcagtgggc ggcgatcgcc tcgattgcga ccaagatcgg ctgcacggcg
    34081 caaacgctgt cgaactgggt ccgccaggcc gagcgcgact agggtaagcg cgccggcctc
    34141 accaccgacg agcgcgagcg gctcaaagcg ctcgagcgca aggtccgcga gttgcggcag
    34201 gccaacgaga tcctgcgcaa ggcgagcgcg tattttgcgc aggcggagct cgaccgccga
    34261 tggaagccat gatcagcttc gtcgatgcgt accgcgacaa acacggggtc gagctgatct
    34321 gcagagtcgt tgagatcgcc ccgtcgacct accacgccca cgtcgcccgg cgcgcccagc
    34381 cggagacagc gccgccgcgc gtcaaacgcg atcgtttgct cagcgtcgag atcagaaggg
    34441 ttttcgatga gagcttctct gtctacggcg tgcgcaaggt ctggcgtcag ctcatgcggg
    34501 aaggtcacgg tgtcgcccgc tgcacggtgg cgcggttgat gaagaaagaa gctctgcaag
    34561 gggtgatccg tggcggccgt gtacgcacga ccgtgagcga tcgcgccacg ccctgtccgc
    34621 tcgaccacgt caatcgcgag ttccgggctc cacggccgaa cgcgctgtgg gtctcggact
    34681 tcacctacgt cgcgacctgg acggggttcg tctacgtcgc gttcgtcatc gacgcctacg
    34741 cccgtcgcat cgtcggctgg cgcgtcagcc ggtcggcgca cgcaggcttc gtgctcgatg
    34801 cgctggagca ggcactccac gatcgcaagc ccgtctgtgg cagcggcctc gtccaccact
    34861 ccgatcgcgg cgtgcaatac gtctcgatca aatacaccga gcgccttgcc gaggccggcc
    34921 tcgtgccgtc cgtcggcagc gtcggcgaca gctacgacaa tgccctcgcc gagacgatca
    34981 acggcctcta caaagccgag gtgatccacc ggcgcgggcc gtggcgttcg ctcgaagccg
    35041 tcgagtacgc cacgctcgaa tgggtcgact ggttcaacaa ccgcaggctt ctctcatccg
    35101 tcggccacgt gccgccggcc gaagcagaag ccgcctacta cgcgagcctg gaggagaccg
    35161 agaagattgc cgcctgactc aatccaacca gcctccgagg aagtcgggac agttcagtgt
    35221 cagggtccgg acgaacttca gaaagttcgt tcacgcgaca cgcgaggcgg taggcacggc
    35281 agtcaggtca agcttgaccc acaccggcac gtgatctgac ggtttctccc agccgcggac
    35341 gtgcttgtcg acggcggcgc gctcgaggcg gtcggcggct tggggcgaga gcatcaggtg
    35401 atcgatgcgg atgccgttgt tcttctgcca ggcgccggcc tgatagtccc agaaggtgta
    35461 gacgccgggg ccgggctcgc aggcgcggag cgcgtccgtg aggccgagcg ccacgaggcg
    35521 gcggtaggct gcccgcgatt ccggctgata gagcgcatcc tccgtccagg ctcccggcga
    35581 cttggcgtcg gcgggctcgg ggatgatgtt gtagtcgccg gcgagcacca aaggctcctc
    35641 gagcgcgagc aggtgccgcg catgcgcctc gagccgggca gcccaagcct gcttgtagga
    35701 gaatttctcg gtgccgatcg ggttgccgtt cggctgatag agcgaggcta ttcgcaccgc
    35761 gcccgcgggt accgagatag tcgcctcgat gtagcgtgcc tgctcgtcgg cgtcgttgcc
    35821 gggaagcccg cgtgtcacgt cctctagcgg gcgcttcgag aggatcgcga cgccgttgaa
    35881 gcccttctgg ccgtgcacgg cgacgttgta gcccttcgcc tcgatttcgg cggcggggaa
    35941 cccctctgtg acgctcttga tctcctggag gcagaccaca tcggggttgc gctcgccgag
    36001 ccatgtgagc agcgtctcga tgcgggcctt gacgccgttg atgttccagg tggcgatgag
    36061 catcggtttg ggtccggcgg gttgcggctg tcagcaagct ctgcatggct cccggtcgct
    36121 tcgcaatctt gaagtcgcag ccgatccgca ttagccgggg atgacaaccg caacggggac
    36181 gaccgctgac ccacctcgca cccatgctcg atctcgccgg cgcctcggga tcgggccgcg
    36241 ccctcctgct gctcgccatc gccctgctga tcggcgggct cgtcaccgga tttctgtccg
    36301 gtctcctcgg catcggcggc ggcggcatcg tcgcgccgat cctctacgag gtgttcggta
    36361 tcatcggggt cgatccggcc gtgcggatgc attgtgcggt cggcaccgcg cttgctgtga
    36421 tgatcccgac ttcgatccgc tcgttcatgg cccacaaggc gcgcggtggc gtcgacatgg
    36481 aggcgtggcg ccgactcggc gtcggcgtgg tggccggcgt tgttatcggc gcgctcgtcg
    36541 ccagcgtctc gcctggtacg ctcctcaagt gggtgtgggt cggctttgcc accgtgatga
    36601 gcctgaagct cgtcttcggc aaggagcagt ggcggctcgg agagcagttg ccgaatgccc
    36661 cgctgaccga actctacggc gccttcgtcg gcgtcatctc gacattgctc agcatcggcg
    36721 gcggcgcctt catcaatacg ttcatgacgc tctatggccg caccatccag acggcggtcg
    36781 gcacggcatc ggggatcggg ccactgattg ccattccagg cacgatcggc ttcctctggg
    36841 cgggtctcgg cgttgccggg cggccggagc tgtcgctcgg ctatgtcaac ctcgtcgggt
    36901 tcgtgctggt cggcgcgacg agcgtgctcg cggcaccctg gggtgtgcgg ctcgcccacg
    36961 gcgtctcgcg ccgcgcgctc gagcttgcgt tcgccgcaat gctgtcgacg atcgggctca
    37021 ggttcctctg gacgctgctg ttcgagtagc aggttgcgtg cgtctgacgg attctggcag
    37081 agtgctgcat ggctcagacc atactccgcc gcccggcgac ctatgccgat ctcgaggccc
    37141 tgccggacaa catggtgggg cagatcctct tcggcacgct gcatgcccat ccgaggccga
    37201 cgccaaggca catccgggcc tcgacgaagc tcggcgccga actcgacgga ccgttcgatc
    37261 gcggacgggg tggtccgggc ggctggctgc tccttatcga acccgacttg cattctggcg
    37321 agcacgtcgt cgtgccggcc aactacaact cgccgggcca gatcgtcatc ggcggccatg
    37381 cgctc
//
LOCUS       DAORNH010000010        35245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_927_length_35245_cov_2.685877, whole genome shotgun sequence.
ACCESSION   DAORNH010000010 DAORNH010000000
VERSION     DAORNH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 35245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35245
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_35245_cov_2.685877"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK264_02030"
     CDS             <1..884
                     /locus_tag="PK264_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74706.1"
                     /translation="KRIETLRRNIRELEERIVQLREKKLVAPKTGMLPGIITDTVDSL
                     TKDIAEAQKRIDLNREEIEKSKELVREALAKVGIMMNREQIDLLLEGVLGGDLMRLMA
                     VFNSAKLIDAQLAKLLNESGENLGAARKYFAMHAALFAILVHAQDQMIAKIDKQYLPK
                     LEAITRDIAAARTRTAELLRGENRPDQRRALEANRESQRLAEDAARSYRRYLLMQREQ
                     IARARARAAHDLKIADNTFETVEASFQLRTLMRDATSTFDALQRLEAPTFDQIFRNEE
                     LRREFENLTRRLDTPGS"
     gene            1235..2404
                     /locus_tag="PK264_02035"
     CDS             1235..2404
                     /locus_tag="PK264_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HRD74707.1"
                     /translation="MPIINRVAELQPEVAAWRQDIHENPELLYDVHRTAELVRKKLIE
                     FGCDEVVPGVGRTGVVGVIKGRKSGSGRVVGMRADMDALPIQEITGLPYASKTPGKMH
                     ACGHDGHTAMLLGASKYLCETRNFDGTAIVIFQPAEEGGAGGKAMVDDGMMDRWGIQE
                     VYGMHNMPGIPTGHFAIRPGPMLAASDRFDVTIEGRGAHAAKPHQGIDPVAIGCQLHA
                     AFQTIVSRNVDPIDSAVVSMTMFHAGDAMNVIPQTAKLAGTVRTLKQSVREKVMARMR
                     TISEGLAAAYDAKIELKYSLGYPVTVNHVKETAFAANVARQVVGEKAVDDNTPASLGG
                     EDFAYMLEARPGAFIYVGNGDTAGVHHPAYNFNDEAIPSGVSFWAKLAEAAMPAR"
     gene            complement(2436..3008)
                     /locus_tag="PK264_02040"
     CDS             complement(2436..3008)
                     /locus_tag="PK264_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD74708.1"
                     /translation="MQQLTTRPATYAEVEALPPKVVGEILFGVLHTQARPAPRHGYAA
                     GILGSEFIGTYRFGRGGPGGWVVIVEPELHFGSHVVVPDIAGWRKERLPAMPDTAWIV
                     TPPDWVAENLSPSTMRIDRTDKLAVYASFGVSHAWYVDPLVLTLEVFELVASRWQIER
                     TFKDADPVCAPPFEAHTFPLDILCPEAPPA"
     gene            complement(3087..5363)
                     /locus_tag="PK264_02045"
     CDS             complement(3087..5363)
                     /locus_tag="PK264_02045"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRD74709.1"
                     /translation="MSTGAIGAAVSRRLALITDKGAILTLFSGTLFLSALLLFSVQPL
                     FAKLVLPKLGGSPSVWAVSMCFFQAVLLAGYCYAHALNRFVPHRLAPLVHLGVLAIAV
                     LALPVGLPANLAEPTSSNTYVWLIGLLAVGVGLPFFAVSANAPLLQSWFARTGHPHAN
                     DPYFLYGASNLGSLAALLLYPVLIEPLIGLGQQASMWAAGFVVLGLAIAVCGLAMLVN
                     RTADGPAALPEAPAAAPPVIWADRAVWIGLAAVPSGLLVAFTSYVTTDIASAPFLWVL
                     PLAAFLATFILVFRPVPVIPHRWMLMLQPLTVIAVLFGISTPGSTGWMVAAIGGSAAF
                     LVTTMVCHKELFDRRPASAHLTEFYLWMSAGGVIGGVFAAIIAPQVFSTIWEFPILVV
                     LGMACRPGVMRDYLESDRRQLLALAIGGLLALMAIEWLMIKRIIPVGQTPRILALVVL
                     AVLVVLAAARPLRQLTLMAIAGMSISILPSAMNRGHAERSFFGVHRVAMLGQNEMRVL
                     FHGTTIHGAERIRNADGTPVTNPVPATYYYTPMARAAEVARQTTGRTGGDLRAAVVGL
                     GTGALACSAKAGERWRFYEIDPLVADIARDARKFTFMSHCQPNAEIVLGDARLTLDKV
                     AAGSLDYLVIDAFASDAVPVHLMTREAITLYLSKLSPNGILAMHVSNRHLDLHSVATS
                     TAKSVAGTLVALADDRFRDAGLDRSPSHVVLVTRSEPAHAALLALPYASEVKAASVTP
                     WTDDFSDILGAIWRNARR"
     gene            5572..6201
                     /locus_tag="PK264_02050"
     CDS             5572..6201
                     /locus_tag="PK264_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRD74710.1"
                     /translation="MTIHPTNTLNRATAQPVTLTSDRRRHKRVALALLGRFMRANRME
                     YPCKLQDISVGGGAILAPVPVTLNERIVAYFDHLGGVEGNVLRIFDGGFVMQFSATQH
                     KREKIAAQLTFLINRDTVDGIEERRHERSIPKNSVSSLKLGDGVILPCQVMDVSISGA
                     SIGTEARPAVGTDVLLGKLRARVMRHHEHGIGVQFLDIQNPSALRKYFG"
     gene            6328..6987
                     /locus_tag="PK264_02055"
     CDS             6328..6987
                     /locus_tag="PK264_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HRD74711.1"
                     /translation="MKRSAIGIFAVLISQSAGSVLADPRTASSAQDRASPFMRVHGPT
                     KPPYGFVRFCATNPVECARFPLDHVRFHLTPDRMRELVELNRDINQRIEATTDMDAHG
                     VEEYWTLPQSRGDCEDYALLKRHILISRGWPPGALLMTVVRDEKGEGHAILTVRTAQG
                     DYILDNKVDEIRIWSRTPYVFVMRQSYIDPIVWVSLDDQTGSAPPSIAGVRSARPKGA
                     P"
     gene            complement(7090..>7247)
                     /locus_tag="PK264_02060"
     CDS             complement(7090..>7247)
                     /locus_tag="PK264_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRD74712.1"
                     /translation="CGDIIDGVSIEAKGREIFDTVLRVASGKRSKSEELGYGDAEFVP
                     WQIGAVM"
     assembly_gap    7248..7373
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7374..8615)
                     /locus_tag="PK264_02065"
     CDS             complement(<7374..8615)
                     /locus_tag="PK264_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HRD74713.1"
                     /translation="MSTPKSPRTLLLNPADNVVVAVDTVNQGTVVNGVTAAERIPRGH
                     KMAIKAVAKGEPLRKFGQIIGFASGPITPGRHVHTHNCAFGEFDRDYAFAKDARNEAL
                     LPAAERATFEGYNRGNGRSGTRNYIGILTSVNCSATVARLMAEEITRSGLLRDHPGID
                     GVIPLVHGGGCALDLEGEGYEVLKRTQWGYAANPNMGGVVMVGLGCEGFQISRFMEAY
                     GMKETDTFRTFTIQDTGGTRKTVAAGVDAIKSMLPAVARARRETRPASELTLALQCGG
                     SDGYSGITANVALGAAVDLLVRHGGTGVLSETPEIYGAEHLLTRRARSEAVGKTLVEI
                     IDWWRDYCDRNRGSMDNNPSPGNKLGGLTTILEKSLGAAAKGGTTTLEAVYRYAEPVT
                     AKGFVYMDTPGYDPVAATGQVA"
     gene            8713..>9108
                     /locus_tag="PK264_02070"
     CDS             8713..>9108
                     /locus_tag="PK264_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD74714.1"
                     /translation="MNDHVNAFVKGERFRIAGRAGGPLAGLTFAAKDLFDVAGRPSGA
                     GNPDFARGRPLPQRHAWAVSTLLDAGATLVGKTITDEVSLGILGENAFDGTPLNVKAP
                     DRVPGGSSSGSAAAVAGRLCDAALGTDTGG"
     assembly_gap    9109..9140
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9141..9904
                     /locus_tag="PK264_02075"
     CDS             <9141..9904
                     /locus_tag="PK264_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD74715.1"
                     /translation="SFSGLYGIRPTHGRLSLDGMLPQAPSSDTTGWFARDAETFARVS
                     EVMLGEAITPVKPQRLVVAVDAFGFAGPEVSAALAPLVARLGALAGEVREEIMAPPGL
                     SVWGRAQRTLQPYEAWLTFKDWVERENPRMAFGVARGLVLGAMIPESERRWAALMREE
                     ARARLAYLLKPGTILCMPTTPFAAPRRGLPITELEPIRERILCLCAHGGLAGFPQVSI
                     PGATVDGAPIGLSILGSRGSDASLVAIASAMEGAT"
     gene            9901..10314
                     /locus_tag="PK264_02080"
     CDS             9901..10314
                     /locus_tag="PK264_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3225 domain-containing protein"
                     /protein_id="HRD74716.1"
                     /translation="MTTPRDLTPNIPEVVAEISALFERYEQALIDKDVDVLDATFWES
                     PHTIRYALHENGYGFNEIHAHRVARPPGPGIKERRIRLEILTLGRDFATTNLEFKVRG
                     RALIGRQSQTLVRFLGLGWKVVAAHVSTMNETALW"
     gene            complement(10333..11166)
                     /locus_tag="PK264_02085"
     CDS             complement(10333..11166)
                     /locus_tag="PK264_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74717.1"
                     /translation="MGKKLWPPDVDKVGPAAMVRWFDKHPSMLRDDRHVMWLGVYQLW
                     LDRYSDAQHSFAQAIEGFARTARDVEYVNLDLFGLKFVAHWLEGDRNAAVECLTVAME
                     KSHRRHVACYAGLRAGFTIGLLMWFAAREMRDTKLASKAETDLRFGAKWRNGHWPQPL
                     ARLVLGEIAPEEFSKSVLGVDDLFELETRPEGTIRITDMSTFRHPDELQSLYFYLGLM
                     TRPSRARTKGRALLHRVACTKPHGPECEWYLARQLCGMTMSWQPTDALLDRKSAGAKS
                     S"
     gene            complement(11300..11722)
                     /locus_tag="PK264_02090"
     CDS             complement(11300..11722)
                     /locus_tag="PK264_02090"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD74718.1"
                     /translation="MSNAKVSRLNPPSAGPVFGMYSHVSIVEPGTRTAYIAGQVAVDR
                     AGLIVGPNDLAAQVPVVFANLEGILADLRASFDDVIELRTYVVGRENLDAWFKARTAV
                     YTRLYPNGKWPPNTLVIVSALNRPELRLEISATVALGA"
     gene            11882..12586
                     /gene="pcaH"
                     /locus_tag="PK264_02095"
     CDS             11882..12586
                     /gene="pcaH"
                     /locus_tag="PK264_02095"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HRD74719.1"
                     /translation="MTAYPTKSLAAHPPFKYPAYKGTIDRAPTRPLVIIPQTMTELTG
                     PMYGHESVKAGDSDLTRQHAGDAIGERIIVSGRVLDESGRPVPNTLLEVWQCNAAGRY
                     RHSGEQHDAPLDPNFSGAGRAVTDAEGRYRFTTIKPGAYPWPNDRNSWRPAHIHFSLF
                     GPTILSRLITQMYFPGDPLLPLDTIFMSAPEGARDRLISRFDIETTQPNWALGYQFDI
                     VLRGRAETPTEAPHHD"
     gene            12579..13169
                     /gene="pcaG"
                     /locus_tag="PK264_02100"
     CDS             12579..13169
                     /gene="pcaG"
                     /locus_tag="PK264_02100"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HRD74720.1"
                     /translation="MTDVSPSMTIGPFFHFAMTEGSRGDGDIATGNMLTPDAAGERIR
                     IVGRVIDGKNLPLTGALIELWQADGQGRYAAPGDPRRASNSSFRGFGRCPTDDAGNFV
                     FDTVRPGAVPGPGGKAQAPHIAVILHGRGLLRNAVTRIYFPDEAANATDHVLSLVPSE
                     ARSILIARREADQGGVKVYRFDIELQGPRAMQFFEV"
     gene            13173..14363
                     /gene="pobA"
                     /locus_tag="PK264_02105"
     CDS             13173..14363
                     /gene="pobA"
                     /locus_tag="PK264_02105"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HRD74721.1"
                     /translation="MARRTEVGIVGAGPAGLMLAHLLAREGISSVIVEARSRSYVEGR
                     IRAGVLEHWVADLLAETGVGVRMQREGLLHHGIELRFAGRAHRLDLSELTSGRAITVY
                     GQHEVVKDLIAARLAAGQEIVFEAADVQVHGIGGDAPSLTYAWDGRTEQIDCDLIAGC
                     DGFHGVCRASIPAEKLAIFERVYPFAWLGILAQSRPVSEELIYASHDDGFALFSMRAP
                     TVTRLYLQCRPDEDLAAWSDERIWGELERRLGGASGALERGPVLQRGVTGMRSFVVEP
                     MQYGRLYLAGDAAHIVPPTGAKGMNLAMADVRVLSAAMRDFLKSGSRRLLDAYSARCL
                     KRIWRAERFSWWMTSLLHRFPDEDAFGYRMRMAELDYITSSKAAATALAENYVGLAFE
                     APEG"
     assembly_gap    14536..14545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14546..15337
                     /locus_tag="PK264_02110"
     CDS             <14546..15337
                     /locus_tag="PK264_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HRD74722.1"
                     /translation="GTARGPAAILAASHQLELFDEELWREPWRDFGIATLREPVIDKS
                     VSRALDQLETIVESVVAAGRFPLVLGGEHSLTPGAIRPLVKRHEDLVVLQLDAHADLR
                     DGYLGETFSHAAAMRRVLDHPGVSLVSVGIRVFSSEEAAFLEGAGERVRIHWGKDQAR
                     WDIEEIVRPLRGKRVYVTFDIDALDSAVMPATGTPVPGGVPFLTALAILRRAAEVSTI
                     VGGDVVELAPIAGLHACDYTAAAVASKIMTYALSGTGVRACPPRR"
     gene            15410..16408
                     /locus_tag="PK264_02115"
     CDS             15410..16408
                     /locus_tag="PK264_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD74723.1"
                     /translation="MVSPVEVSIIVVSYNTKAMTLACLASVRAETRASFELIVVDNAS
                     SDGSAEAIAAADPEAVIIPSRENLGFARANNLAARRARGRYILLLNPDTLVHEGAIDR
                     LLAFAKARPAARIWGGRTTFGDGRLNPASAWGEMTVWNLFCRAAGLTGLFPRTTLFNG
                     EALGGWDRASERDVDIVSGCFFLIERAFWQQLGGFDSAFFMYGEEADLCLRARELGAR
                     PRVTPEATIVHYGGASETAREAKLVKLLAAKSMLIRRHWPRGRRLVGLALLSAWPLTR
                     ALATAIAIPLNAGAWRACREWRAVWRARARWLNGFPIMTSQASLRVTARPGTGGAP"
     gene            16405..17229
                     /locus_tag="PK264_02120"
     CDS             16405..17229
                     /locus_tag="PK264_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74724.1"
                     /translation="MTTASAVTMVRPASEGPVRFTFGTLMTSPEQHAALLATLDAHGF
                     GADDCEVLVVDNARGALRDAYRGLNEMLNAARGDIIILCHQDVRLLKDGREELEARLA
                     DLEAKAPDWGVAGNAGGISPGRLALRITDPHGADLQVGAFPARVQSLDENFLIVRRAA
                     RLSFSRDLTGYHMYGSDICLVADVLGWSCWVIDFHIEHLSGGRKDESFHAAEREFNAK
                     WRRAFRPRLLQTTCTLVSIAGDPVLGFFGRMGVGPIGKISRRLPGAQGFGARRDGQ"
     gene            17253..18743
                     /locus_tag="PK264_02125"
     CDS             17253..18743
                     /locus_tag="PK264_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRD74725.1"
                     /translation="MPSAGVRRRVLGQGLLLFAGFGASQAMSLGRNALLGYLLARGDF
                     GIAASITLTLQTVEILTDLAPDRFLMQARAGEEAHMLGAAHAMSLARGTLIALLLVLA
                     AESLAHFFGVPEARTAFMLMALTPLIKGALHLDHRLAQKRLDNRAYLATEVGGQAAAF
                     IVAAPLLLAYPLPIVVVWLAILQAVVAVGISHVVAEQPFRLVLDRGVLRSALAFGWPI
                     WLSAFPLIAVYQADRLIVGRVLGMEALAAYSAAAMIAMVPGLIAAKVGQAIVLPMLSQ
                     RADDGVGFGRAYRLMLEASVIAAAGYLAVYAMAGGTLLALAFGPQYRGLDLLAAWCGA
                     MWALRMVQAIPGMALMAQGTTRPLLIAGVVRASVVPLVAVVAVMRLPLEMIAVVGLVG
                     ELASLAVLAMALERTAVGAGLRRSTLLRIAMLTPVTGLLALAGHGLPLASTIAVAALA
                     VLAAAFLIAGLMPGIRAMAWASINGMDNSRAADEPPQFYPTAHRNG"
     gene            18816..19898
                     /locus_tag="PK264_02130"
     CDS             18816..19898
                     /locus_tag="PK264_02130"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRD74726.1"
                     /translation="MRPDATTCQSLRGLAIRAMLLALFATGLVPTGPALAARPGEGEI
                     VAPAPRPAIRPPRPGAPSNAVALAERLKRIGAVKSWGYQLNGLNLEAAAASPYDLLIV
                     DATTGLASGRPFEAAEVERLKRKPDGSRRLVVSYLSIGEAEDYRPEYFSPEYMSEDAP
                     DWLMHENPQWKGNRIIRFCNEGWQLTILGDERGRSVYNSIDPSPLFRLIELGFDGVYL
                     DRVDVYKEVAKECGEAERRMVDFVSRLARHARRKEPHFLVIMQNAEELARQAKLIEAI
                     DAVAKEDLFYGADHSERQNEAGMVRTVLDNLRRIKAANRPVFVVDYLKDRTKIDDAKR
                     RILDAGFVPYIAPRALDRLWLPGRDF"
     gene            complement(19917..20402)
                     /locus_tag="PK264_02135"
     CDS             complement(19917..20402)
                     /locus_tag="PK264_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74727.1"
                     /translation="MRSYGSAAPSEAAANEHGAHDPVSGSADASVADIDDDGIFDTGN
                     RWSNVGWATMGFLSGAVFWHAVGFWAFLAGLLSHGPATETSVLGSDCVTLVIDRTTGT
                     TSAEACPELIARLPDGTRNYRGPLLTAELRRARLPASRWEITVNSEGVAGASEERSSG
                     Q"
     gene            20585..21106
                     /locus_tag="PK264_02140"
     CDS             20585..21106
                     /locus_tag="PK264_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD74728.1"
                     /translation="MSSIDIPTTGEARIIGPEDGASFWQPVPANGFVRNILNQAMVGG
                     NASFSMGTQTVAPGCFIREHTHAREEEIIHVLEGEGIARVEGIETPISRGSTVFIGCN
                     RRHHFLNRSASAPFTFLWLMLPGGLDRFFAEIGRPRTPGDPTPAPFPRPDNIAEIERR
                     TVFGWTDKSYDKS"
     gene            complement(21109..21438)
                     /locus_tag="PK264_02145"
     CDS             complement(21109..21438)
                     /locus_tag="PK264_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRD74729.1"
                     /translation="MIFDHRTYRVKPGMIKTHLELYEKHGFATQQKHLGKPVLYATTE
                     VGDVNAYIHVWAYKDLADRTARRAAMWADPDWLAYVKKSGELGALISQENMILVGTSF
                     FKLPGTV"
     gene            complement(21493..22188)
                     /locus_tag="PK264_02150"
     CDS             complement(21493..22188)
                     /locus_tag="PK264_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRD74730.1"
                     /translation="MPPRKKATDILETLTNGRLHTAVVGDVLDQMGFRHQFLPAGFAP
                     LNPAMRLAGRAMPVLEADIFDDGRPSSQGPLARKPFGLMFEALDDLKPGEIYVATGAS
                     LRYALFGELMATRATCLKAAGALLDGYVRDGAQIEAIGFPVFARGLYAQDQGPRGKVI
                     DFRSAIEIAGVRIEPGTLLYGDREGVIVIPREAEEECIARALEKVETENKVGDAIRAG
                     MPAAEAFRKFGVM"
     gene            22293..23276
                     /locus_tag="PK264_02155"
     CDS             22293..23276
                     /locus_tag="PK264_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRD74731.1"
                     /translation="MDHVAVAADRVRGRALRVVLPESTDERIVEAARRLAAEDLATPI
                     LIAESAEALSSAPLTSIHPPTAPSLSRYAAALAARRSATGRAMTDAMARRLVSKPLYF
                     AGAMVAAGDAEAVVAGVANPTRRVIEAGLMTIGLAEGIATPSSYFLMRVPARADRPAR
                     AIIFADCAVVANPTSEELADIAIATAGSAARLLGETPRVALLSFSTHGSATHPDVAKV
                     TSALSLIRARARALAVDGELQGDAALSAAVATKKVKAESAVAGRANILVFPDLDAGNI
                     AYKLVQELAGAEALGPFLQGFARPISDLSRGASVDDIVRTCVITLAQAAVS"
     gene            complement(23271..24020)
                     /locus_tag="PK264_02160"
     CDS             complement(23271..24020)
                     /locus_tag="PK264_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRD74732.1"
                     /translation="MIAPATLTLLALAVLATSFLSGIFGMAGGIILLGVLMLVLDVGQ
                     AMVLFGSTQMAANGWRATLWQDHIYWRVVAGYCLGALAAFLVMRLIAYVPSKAFMYLA
                     LGSMPFLADLLPKRLEPDITRPGMPIICGAILTVLNLLAGATGTLLDVFFQKSALDRK
                     SIVATKAVTQTFAHLLRIFYFGSLMTAFDVTIPWWVYAGAVIPAIVGTTLAARVLEAM
                     TDADFRRWARRIILAVSLTFIARGLWLLAAS"
     gene            24117..25313
                     /locus_tag="PK264_02165"
     CDS             24117..25313
                     /locus_tag="PK264_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD74733.1"
                     /translation="MSEVRAFDGIICIGGEDWWYHNRGHFDFQIMRRLARSVPVLFVN
                     SLGVRMPSVGESRLFLARIGRKLASLKRGLVEAEPGFWVFSPLSVPGAAGAKLSGWAL
                     GPQIRLAARRAGISRPLLWVHCPAGASLVGQIPAAGVVLQRTDRFEAFPEGDRALLAT
                     QIASLKATADLVVYAAPHLMAEETGTVARQLLVTHGVDLETFVSAGRNAADAGPADMA
                     HLPRPRIGFIGGIDAHTFDPPLFLALAARMPDVRFVLVGGTSLPEGWCPLENVTQLGR
                     KPYAEVARYMAAMDCLIMPWNDSPWIQACNPVKLKEYLATGRPVVTRDFAALDGWRDL
                     VRTAATPDAFEAAVRASLTERRDIEAIAARLASETWDAKAALVLDALTDAAAAKSQGT
                     HEPKVA"
     gene            complement(25319..26590)
                     /gene="lpxI"
                     /locus_tag="PK264_02170"
     CDS             complement(25319..26590)
                     /gene="lpxI"
                     /locus_tag="PK264_02170"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRD74734.1"
                     /translation="MPDMMGPVGIVAGGGSLPIEVAESLSRRGRAVHIVAIEGSAGCE
                     VEAYPHTWTRIAAVGRLLGALRAACCREIIFVGRITRPEVRSLRPDLGFFRHLPMFFS
                     ILRDGDDAILTRILRFFERQGFTVLGVPAVAPELVARAGSIGRHEFRASDRADAALGA
                     RLIGAIGPLDVGQAVVVRGGKVIAIEAAEGTDRMLARAAILAAPDASPSGVLVKRPKP
                     GQELRIDLPAIGPPTIAGAIAAQLSGIALAPGGALLLERERLIAAADDGRVAIAGLDE
                     PAASPDRQVRELNVDEVAGRLRAARRRELQIAAEVSRRLAAFNAGAAVAVARRHVLAV
                     SAAEGPLAMLARVRALRQWGDRSSARRDGVVVIRLDEPREPDIAALAAAIGSARLTAV
                     AIDVAWSRSALEVLAGELGSGVTLVSLRASS"
     gene            complement(26583..27386)
                     /gene="lpxA"
                     /locus_tag="PK264_02175"
     CDS             complement(26583..27386)
                     /gene="lpxA"
                     /locus_tag="PK264_02175"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRD74735.1"
                     /translation="MASRIHPTAIVEDGARLGTDVRIGPFCHVGPEAELGPEVELVSH
                     AVVAGKTTIGARTRIFPFASIGHQPQDLKFKGEPVTLAIGADCIIREGVTMNPGTAGG
                     GSATTVGDKCAFLANSHVGHDCHVGSNVIFSNNVMLAGHCTIGDYVILGGGAAVIQFT
                     RVGPHAFLGGMSGLENDLIPYGMALGNRAHLAGLNIIGLNRRGFTREQVHALRRAYRL
                     LFAAEGTLQERVADVAQEYGDNPIVQEIIAFMRVTGKRSLCTPGAGSDA"
     gene            complement(27386..27886)
                     /gene="fabZ"
                     /locus_tag="PK264_02180"
     CDS             complement(27386..27886)
                     /gene="fabZ"
                     /locus_tag="PK264_02180"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRD74736.1"
                     /translation="MSDATPKPGLGHADIMRVMQLLPHRYPMLLVDRIVDMDRDESCV
                     GIKNVSSNEPFFQGHFPAFPVMPGVLIIEGLAQTAGALCIHNLGTEYKPQLVYFMGID
                     KAKFRRPVVPGDQLRYHVRKLRSRGRAWRFYGEARVDGQVAAEAEVSAMIVDTPETQA
                     FLSKQS"
     gene            complement(27959..29029)
                     /gene="lpxD"
                     /locus_tag="PK264_02185"
     CDS             complement(27959..29029)
                     /gene="lpxD"
                     /locus_tag="PK264_02185"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRD74737.1"
                     /translation="MQHPGFFKCAGPFSLTDVAKRTGAELSGTDDAAALITDVRSLSD
                     AGPTDLSFIDNRRYAAQLATTKARACLVAPALAGRVPAHVIKLSTRAPYQGFALALQL
                     FYPEAMKSKAALEEQREGGRTISPSAEIEPGAVIEPGAVIGREARIGRGTTIAAGAVV
                     GYRVHIGRDGYVGPGASITHGLVGDRVIIHAGVRIGQDGFGFAMGPRGHLKVPQIGRV
                     IIQDDVEIGANSAVDRGALKDTIIGEGTKIDNLVQVGHNVVIGRHCVIVGQCGIAGST
                     ELGDFVVIGGQTAIAGHLKIGSGAQIAGKAGVHNNVPAGERWGGIPARPFKIFAREVA
                     ILNQLARKGRAAAGADDEGDAA"
     gene            complement(29458..31755)
                     /gene="bamA"
                     /locus_tag="PK264_02190"
     CDS             complement(29458..31755)
                     /gene="bamA"
                     /locus_tag="PK264_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRD74738.1"
                     /translation="MPSGAIVATAQAQDGRVREIRVVGNKRVEPETVRSYLKFSIGDP
                     YDAGRVDQSIRALFATGLFSDVRIDRSGATVTVSVTENPIINQVAFEGNREIDTATLQ
                     SEVQLKSRSVFTRARVQADVQRILDLYRRQGRFAATVEPKIIELEHNRVNLVFEINEG
                     GATKVKAINFIGNRAFSDSQLRDIISTTQVGLFDFIKGNSIYDPDRLAYDRELLRQYY
                     LKNGYADIRISTGGAELDRDGSGFFITFVVEEGELYRFGGVSIESSLPAINAPSLSGQ
                     LLTKSGQVFNASLMDKSVERLTLAISEQGFAFARVRPRADRDAAARVINLVYVIDEGP
                     RIYIERINVNGNTRTQDYVIRREFRLVEGDAYNPLLVDRAKKRLQGLGFFKNVDIKRR
                     AGSTPDRVVLDVDLVEQPTGELSFGAGYSTAEGVIGDISLTERNLFGNGQFIRLKLSG
                     SIERLQIDLSFTEPRFLDRNLAAGFDVFHKDVNQSNRQAFRNRKTGGSLRLAFPLAEN
                     LWMSTNYTLSRDEIYDVDKDASDGVIASRAIREAEGVTITSSVGTSIAYDARNHPRSP
                     TSGYYLTLSSDVAGAGGDVQYVRFTGEGRGYYPITEKITLVGRVVGGHIFGWGGDEVR
                     LIDMFYKGGETIRGFDRSGIGPRDLSTNDALGGTTFWAATAEVRFPLPLVPDDLGMSG
                     AFFVDAGSLFGASALAKRLNGTADPATGQVVNLADDSSIRLSAGASLLWNSPLGPLRA
                     DFGWALQKEDYDQTQVFRFGASTKF"
     gene            complement(32277..33398)
                     /gene="rseP"
                     /locus_tag="PK264_02195"
     CDS             complement(32277..33398)
                     /gene="rseP"
                     /locus_tag="PK264_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRD74739.1"
                     /translation="MMTFIAFVGLLTIVVFIHELGHFLVARWCGVKVQAFSIGFGREI
                     FGFDDRHGTRWRFAWIPLGGYVKFIDDANEASVPARSGTGTDAAVDWTKVEGSFQGRP
                     LGQRAAIVAAGPIANFILSTLVFTGILMTVGERVKDARISRVVPGSVAESAGFKPGDV
                     ITAVNGTRIETFNDLVKIVGQSAEYELTFTIDRGGAPVTLKATPRFSEEADTFGGKIR
                     VGKIGVAPMEGDFNQRSYPFFESLKRGAWQTWEVVPGTFNYLKDIVTRRQSADQLGGP
                     VRIAEVSGRMAKAGFVPWLYTLALISAAIGLFNLFPIPLLDGGHLLYNAYEAVMRRPL
                     SEQAQEFGFKIGASVVLTLMLFVMWNDRGILQKWLTFGG"
     gene            complement(33659..34492)
                     /locus_tag="PK264_02200"
     CDS             complement(33659..34492)
                     /locus_tag="PK264_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRD74740.1"
                     /translation="MPELSSLFGRDLTQRTISGLALAAAALLLTYLGPLPFAGLVLAI
                     AMALTWEWGRVVRDGEIDAVMIVHGIAILAAIVATFLKAPAIAILIVAMGGVLAGVVA
                     YGATSRLSAIGVLYVGLPAIALISFRSDPVWGFRAVLLLFVCVWGTDVAAYFSGRAIG
                     GPKLWPRVSPNKTWAGFVGGVGTSAIAGAILSRFVEQGSALRLALTGLGLGIVAQLGD
                     LAESALKRKHNVKDASDLIPGHGGFLDRVDGLVIAAVVAAAAAVLINVHSPARALLVG
                     Q"
     gene            34789..>35245
                     /locus_tag="PK264_02205"
     CDS             34789..>35245
                     /locus_tag="PK264_02205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD74741.1"
                     /translation="MHPALELIPSDEAPPREVDVVVIGGGIVGAAATYAVAKAGQRVA
                     LVEKGYVGAEQSSRNWGWCRQQNRDARELPLAMRSMELWESLGGEIGRDLGFRRCGLV
                     YATTDPAQVKQWESWRSVARSFGVDTRMLTGAEAARMSGTTARPWLAGIH"
BASE COUNT         5962 a        11543 c        11686 g         5886 t
ORIGIN      
        1 agaagcgcat cgagacgctg cgccggaaca tccgcgaact cgaggagcgc atcgtccagc
       61 tccgagagaa aaagctcgtc gcgccgaaga cgggcatgct gccgggtatc attacggata
      121 cggtcgacag cctgacgaag gatatcgcgg aagcccagaa gcgcatcgat ctcaaccgtg
      181 aggagatcga gaagtcgaag gagctcgtgc gcgaggcgct ggccaaggtc ggcatcatga
      241 tgaaccgcga gcagatcgac ctgctgctcg aaggcgtgct cggcggcgat ctgatgcggc
      301 tcatggccgt gttcaactcg gcaaagctca tagacgccca gcttgcaaag ctcctcaacg
      361 agagcggcga gaacctgggt gctgcccgca agtacttcgc catgcacgcg gcgctgttcg
      421 caatcctggt gcacgcccag gaccagatga tcgccaagat cgacaagcag tacctcccga
      481 aactcgaggc gatcacgcgc gacatcgcgg ccgcacgaac gcgcaccgcc gagttgcttc
      541 ggggcgagaa ccggcccgac cagcgccgcg cgctcgaggc gaaccgcgag agccagcggc
      601 tcgccgagga tgcagcgcgc agctatcggc gctatctgct catgcagcgc gaacagatcg
      661 cgcgcgcgcg cgcccgggcg gctcacgatt tgaagattgc cgacaacacc ttcgagacag
      721 tggaagcgag cttccagctg cgcactctca tgcgcgatgc gacctccacc ttcgatgcgc
      781 tgcagcgact tgaggcgccg accttcgacc agatcttccg caacgaggag ctacggcgcg
      841 aattcgagaa cctgacgcgc cgcctcgata cgccgggctc gtgacggtgt cgtcgagtgt
      901 gcccgctcgc tagtgtcccg atcgcgaagt tcgctaccac gagacgcgaa ctcggacgaa
      961 cgtcgcgatc gatagggaca ctaggaatta catgagctta gtgtggcgtt atgttcggaa
     1021 gttcggttct cgagaatgcc acacgttgaa tacgaacttc cgaaccgcca cacttgcacc
     1081 cgggcggcat tgttgcgccc atgcaactca atcaaccgaa acacctggct cgcgacagcg
     1141 cgtggttgtc gcgagcgtta gcttgtatgc tgcctgcgtt gacgtattgg taagtcatcg
     1201 ctcatatctc agcggccaga ttggaggaac cagaatgccc atcatcaacc gcgtcgcaga
     1261 attgcagccc gaggtcgccg cctggcgcca ggacatccac gagaatccgg agctgctcta
     1321 cgacgtgcat cgcaccgccg agctcgtccg caagaagctc atcgagttcg gctgcgatga
     1381 ggtggtgccg ggcgttggcc ggaccggggt ggtcggcgtg atcaaagggc gcaagtcggg
     1441 gtcgggacgc gtcgtcggta tgcgcgctga catggatgcg ctccccatcc aggagatcac
     1501 agggctgccg tatgcctcca aaacgcccgg gaaaatgcac gcctgcggac atgacggcca
     1561 cacggccatg ctgctcggcg cgtcgaagta cctctgcgag acgcgcaatt tcgacggcac
     1621 cgcgatcgtc atctttcagc cggctgaaga gggcggcgcc ggcggcaagg caatggtcga
     1681 tgacggcatg atggaccgct ggggcatcca ggaggtctat ggcatgcaca acatgccggg
     1741 catccctacg ggccatttcg ccattcggcc gggtccgatg ctcgccgcct ccgaccgctt
     1801 cgacgtgacc atcgagggcc gcggcgcaca tgcggcgaag cctcatcagg gcatcgatcc
     1861 ggtggcgatc ggctgccagc tgcatgccgc gttccagacc atcgtctcgc gcaacgtgga
     1921 cccgatcgac tcagctgtcg tctcgatgac catgtttcat gccggcgacg ccatgaacgt
     1981 gatcccgcag accgcaaagc tcgccggcac cgtgcgcacc ctgaagcagt ccgtgcgcga
     2041 gaaggtgatg gcgcgcatgc gtacgatttc cgaaggtctg gcagctgcct acgacgccaa
     2101 gatcgagctc aagtactcac tcggctatcc ggtgaccgtg aaccacgtga aggagaccgc
     2161 cttcgccgca aacgtggcgc gacaggtggt cggcgagaag gcggtcgacg acaatacgcc
     2221 ggcgagcctc ggcggcgagg acttcgccta catgctggaa gcacggcccg gcgcgttcat
     2281 ctatgtcggc aacggcgata cggccggcgt gcatcatccg gcctacaact tcaacgatga
     2341 ggcgatcccg tcgggcgtgt cgttctgggc gaagctcgct gaagcggcga tgccggcacg
     2401 ctgaggccgg cacgctgagg cctgtcaagg ggctgttagg ctggcggcgc ctccgggcag
     2461 aggatgtcga gcgggaaggt gtgcgcctcg aacggcgggg cgcatacggg atcggcgtcc
     2521 ttgaaggtgc gctcgatctg ccagcgtgag gcgaccagtt cgaacacctc gagagtgagc
     2581 acgagcggat cgacgtacca ggcatggctg acgccaaagc tcgcgtagac tgcgagtttg
     2641 tccgtgcggt cgatgcgcat cgtcgagggc gaaaggttct ccgcgaccca gtccggcggg
     2701 gtaacgatcc aggccgtatc gggcattgcg ggcagccgct ccttccgcca cccggcgatg
     2761 tccggcacga cgacgtgcga accgaagtgc aactcaggct ccacgattac gacccagccg
     2821 ccaggaccac cacgtccgaa ccgataggtg ccgataaact cgctgccaag gatgcccgcc
     2881 gcatagccgt ggcgaggcgc cggacgtgcc tgcgtatgca gcacgccgaa caggatctcg
     2941 ccgacgactt tgggcgggag cgcctcgact tcggcgtagg tcgctggtcg cgtcgtcaac
     3001 tgctgcatcg cggcggcctt tcggccgtac catcgggccc tgcagatacg actccatccg
     3061 gtccggcgag gccacccgat cgccctctac cgccgcgcat tccgccagat cgcgccgagg
     3121 atgtccgaga aatcgtcggt ccacggcgtg acagaggcag ccttcacctc gctcgcatag
     3181 gggagcgcga gcagcgcggc atgcgccggc tcggaccgtg tcacaagcac cacgtgcgag
     3241 ggtgagcgat ccaatccggc gtcgcggaag cgatcatcgg cgagcgcgac gagcgttccg
     3301 gcaacggact tcgccgtcga ggtcgcgacc gagtgcaggt cgaggtggcg gttcgagacg
     3361 tgcatcgcga ggatgccgtt tggcgagagc ttcgagaggt agagggtgat cgcctctcgc
     3421 gtcatgagat gcacgggtac cgcgtccgag gcgaaggcgt cgatcaccag atagtcgagg
     3481 ctgcctgccg cgaccttgtc gagcgtcagg cgggcatcgc cgaggacgat ctcggcgttc
     3541 ggctggcagt gcgacatgaa tgtgaacttg cgggcatcgc gggcgatgtc tgccaccaag
     3601 gggtcgatct cgtagaagcg ccagcgctcg cccgccttgg ccgagcaggc tagtgcgcct
     3661 gtaccgaggc cgacaacggc ggcgcgcagg tcgccacctg ttcgtccggt cgtctggcga
     3721 gcgacctcgg ccgcgcgtgc catcggcgta tagtagtagg tcgccggcac cgggttcgtg
     3781 acgggcgtgc cgtctgcgtt gcggatgcgc tcggcaccgt ggatggtcgt gccgtggaag
     3841 aggacgcgca tttcgttctg cccgagcatg gcgacgcgat ggacgccgaa gaagctccgc
     3901 tcggcgtggc cgcggttcat cgccgacggc aggatcgaga tcgacatccc ggcgatcgcc
     3961 atcaaggtga gctggcggag cgggcgcgcg gcggcgagga cgaccagtac cgccaggacg
     4021 accagagcca ggatgcgtgg tgtctggcca accggaatga tgcgcttgat catcagccat
     4081 tcgatcgcca tcagcgcgag gagcccgccg atggcgagcg ccagcagctg tcgccggtcg
     4141 ctctccaggt agtcgcgcat cacgccaggc ctgcaggcca tcccgaggac gaccaggatc
     4201 gggaactccc agatcgtgct gaacacctgc ggtgcgatga tcgccgcgaa gacgccgccg
     4261 atgacgccgc cagctgacat ccacaggtag aactctgtga gatgggccga ggccggacgc
     4321 cgatcgaaca gctccttgtg gcagaccatc gtcgtcacca ggaacgccgc agagccgccg
     4381 atcgccgcca ccatccagcc cgtgctgccg ggcgtcgaga taccgaacag cacggcgatc
     4441 acggtcagcg gctgaagcat gagcatccag cggtgcggga tcaccggcac cggccggaac
     4501 accaggatga aggtcgcgag gaacgccgcg agcggcagca cccagaggaa gggtgccgag
     4561 gcaatgtcgg tcgtcacgta gctcgtgaag gcgaccagca ggcctgacgg tacggcggcc
     4621 agtccaatcc agacggcgcg gtcggcccag atgacgggag gcgccgcggc cggagcctcg
     4681 ggaagggcag cgggaccatc ggccgtacgg ttgaccagca tagcgaggcc gcaaacagca
     4741 atggcgaggc caaggacgac gaagccagcg gcccacatcg acgcctgctg gccgagcccg
     4801 atgagcggct cgatcagcac cggatagagc aatagggccg cgagactgcc gaggttcgac
     4861 gcgccgtaga ggaagtaggg gtcgttcgca tgcgggtggc cggtgcgcgc aaaccaggac
     4921 tggagcagcg gcgcatttgc cgagacggca aagaagggga ggccgacgcc gacggcgagc
     4981 agcccgatga gccagacgta ggtgttcgag ctcgttggct cggcgaggtt cgccggcagg
     5041 ccgacgggca gcgccagcac agcgatcgcg agcacgccga gatggacgag cggcgcgagg
     5101 cggtgcggca cgaagcgatt gagggcatgc gcgtagcagt agccggcgag cagcacggcc
     5161 tggaaaaagc acatggagac cgcccacacg gacggtgacc cgccgagctt cgggagaacg
     5221 agcttcgcaa acagcggctg gaccgagaag agcaggagcg ccgacaggaa gagcgtgccg
     5281 gagaagagcg tcaggatcgc gcccttgtca gtgatgagag cgaggcgccg gctgaccgcc
     5341 gcgccgatgg cacccgtgct cattctggtc ccccttacgc aagcacttcg aggcccgccg
     5401 actttagcgg ggattggttg aggcgatctg aaagcgcaat tgcctgatct gtcccagaac
     5461 cgggcgcgtt gttaggggat ccttaatgcg cctcgccgaa catcagggcg aggccgaaac
     5521 gaggccaatt cctaaacccg ttcgcgcgag atcgccgcgc cgaggatgga aatgaccatt
     5581 cacccgacga acaccttgaa ccgtgcgacc gcgcaaccgg tcaccctgac ctccgatcgc
     5641 cgccgtcaca agcgcgtcgc cctcgcgctg ctcggccgct tcatgcgcgc gaaccgcatg
     5701 gaatatccct gtaagctgca ggacatctcg gtcggcggcg gcgcgatcct ggcgccggtc
     5761 cctgtgaccc tcaacgagcg catcgtcgcc tatttcgatc atctgggcgg cgtcgagggc
     5821 aacgtcctgc gcatctttga tggcgggttc gtcatgcaat tcagcgcgac ccagcacaag
     5881 cgcgagaaaa tcgcggcgca gctgaccttt ctcatcaatc gcgatacggt cgacggcatc
     5941 gaggagcgcc gccacgagcg ctcgatcccg aagaacagcg tctcgagcct gaagctcggc
     6001 gacggcgtca tcctgccctg ccaggtgatg gacgtctcca tctcgggtgc ctccatcggc
     6061 accgaggcgc gccctgccgt gggcacggac gtgctcctcg gcaagctccg agcccgcgtg
     6121 atgcgccatc acgaacacgg catcggcgtg cagtttctgg acatccagaa cccgtcggca
     6181 ctccggaagt attttggctg aggccgcgag gcggatcaat ttcaattcga gacttattgc
     6241 cggggcattt ccggcaattc cgcctgcccg ccttgagggg aggctgatgt ttgttgcgta
     6301 gagtgatcgt gggttggggg tccggtcatg aagcgtagcg caatcggcat attcgcagtc
     6361 ctgatatcgc aatcggctgg ctcggtgctc gccgatccgc gcacggcgag ctcggctcag
     6421 gatcgcgcga gccctttcat gcgggtgcat ggaccgacca agccgcccta cggcttcgtc
     6481 cggttctgtg ccaccaaccc ggtcgaatgc gcgcgcttcc cgctcgacca tgtgcgtttt
     6541 catttgacgc cggatcgcat gcgcgagctc gtcgagctca atcgcgacat caatcagcgc
     6601 atcgaggcca cgacagacat ggacgcccat ggcgtcgagg aatactggac cctaccgcag
     6661 tcgcgcggcg actgcgaaga ctacgcgctc ctgaagcgcc acatcctgat ctctcgaggc
     6721 tggccgccgg gcgcactgct catgacggtc gtgcgggacg agaagggcga aggccacgcg
     6781 atcctcaccg tgcggaccgc gcaaggcgac tacatcctcg acaacaaggt cgatgagatc
     6841 cgcatctggt cgcggacgcc ctatgtgttc gtcatgcgcc agtcctacat cgatcctatc
     6901 gtctgggtgt cgctcgacga ccagaccggc agcgcgccac cctccatcgc cggcgtccgc
     6961 agcgcccgcc ccaagggcgc gccatagcaa gtttcgtcca ggctcagagc acctgccgct
     7021 cccccatgct ccgggccttg cctcgggccg ggttccgatg atcggacccg gcccattttc
     7081 ttgcccgcct cacatcaccg cgccgatctg ccagggcacg aactcggcgt cgccatagcc
     7141 gagttcttcc gacttggagc gcttgccgga ggcgacgcgc aggacggtgt cgaaaatctc
     7201 gcggcctttc gcctcgatcg agacgccatc gatgatgtcg ccgcagtnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcgacc
     7381 tggcccgttg ccgcgacggg atcgtagccg ggcgtgtcca tgtagacgaa gcccttggct
     7441 gtcactggct cggcgtagcg gtagaccgcc tcgagcgtgg tggtgccgcc cttcgccgcc
     7501 gcgccgagtg acttctcgag gatggtggtg aggccaccga gcttattgcc ggggctcggg
     7561 ttgttatcca tcgagccgcg gttgcggtcg cagtagtcgc gccaccagtc gatgatctcg
     7621 acgagcgtct tgccgacggc ctcgctgcgg gcgcggcgcg tcaggagatg ctcggcccca
     7681 tagatttccg gcgtctccga gagcacaccc gtgccgccgt gacggacgag caaatcgacc
     7741 gcggcgccga gcgctacgtt cgccgtgatg cccgagtagc cgtccgagcc gccgcactgg
     7801 agtgccaggg tcagctcgga agccggccgc gtctcgcgcc gcgctcgggc gacagctggg
     7861 agcatggact tgatggcatc gacgccggcg gcaacagtct tcctcgtgcc gcccgtatcc
     7921 tggatcgtga aggtgcggaa cgtgtccgtc tccttcatgc cgtacgcctc catgaagcgg
     7981 ctgatctgga agccctcgca gccgaggccg accatcacga cgccgcccat gttcgggttc
     8041 gccgcatagc cccattgcgt gcgcttcaga acctcgtagc cctcgccctc gaggtcgagc
     8101 gcgcagcccc cgccgtggac gagcgggatc acgccgtcga tgccgggatg atcgcggagg
     8161 aggccggagc gcgtgatctc ctcggccatc aggcgggcga cggtcgccga gcagttcacg
     8221 ctcgtcagga tgccgatgta gttgcgcgtg cccgagcggc cgttgccgcg attgtagcct
     8281 tcaaacgttg cgcgctcggc cgcgggcagc agcgcctcgt tgcgggcgtc cttggcgaag
     8341 gcatagtcgc ggtcgaactc gccgaaggcg cagttatgcg tgtggacgtg ccgaccgggc
     8401 gtgatcggcc ctgaggcaaa gccgataatc tgcccgaact tcctgagcgg ctcgcccttg
     8461 gcgacggcct tgatcgccat cttgtgcccg cgcgggatac gctcggcagc ggtgacgccg
     8521 ttgacgaccg tcccctggtt cacggtatcg accgcgacca cgacgttgtc ggccggattg
     8581 aggaggaggg tgcggggcga cttcggggtg gacatcgttg gctcgctcga tcgggctgga
     8641 acgctgacgg tccggtgggg cagtatcgcc gtcggcaaca gcggttgggt cacagcggtt
     8701 gggtaagggc tcatgaacga ccacgtcaac gccttcgtca agggcgagcg cttccgtatc
     8761 gcggggcgtg cgggaggccc gctcgcgggg ctcacgttcg cggcgaagga cctgttcgac
     8821 gtggcgggcc ggccgtcggg cgcaggaaat cccgacttcg cacggggccg cccgctgccg
     8881 cagcgccatg cctgggccgt ctcgacgctg ctcgatgccg gcgcgacgct ggtcggcaag
     8941 acgatcacgg acgaggtgtc gctcggcatt ctcggcgaga acgccttcga cgggacgccg
     9001 ctcaacgtga aggcgccgga ccgcgtgccg ggtggctcgt cgtcgggatc ggcagcagcc
     9061 gtagccggcc ggctctgcga tgcggcgctc ggcaccgaca ccggcgggnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn cgagcttttc gggtctctac ggcatccgcc cgacgcatgg
     9181 ccgcctgtcg ctcgatggca tgcttcctca ggcaccctcg tcggatacga ccggctggtt
     9241 cgcgcgcgat gccgagacgt tcgcgcgcgt ctcggaggtg atgctcggcg aggcgatcac
     9301 gcccgtgaag ccgcagcgtc tcgtcgtcgc cgtcgatgcc ttcgggttcg cgggacccga
     9361 ggtcagcgcc gccctcgccc cgctcgtcgc gcggctcggt gcgctcgcgg gcgaggtgcg
     9421 tgaggagatc atggcgccgc cgggcctctc ggtctggggg cgcgcgcaac ggaccttgca
     9481 gccctacgag gcctggctca ccttcaagga ttgggtcgaa cgggagaacc cgcgcatggc
     9541 gttcggcgtg gcacgcgggc tcgtccttgg cgccatgatc ccggagagcg agcgccgctg
     9601 ggccgccctg atgcgcgagg aggcgcgggc ccgcctcgcc tacctcttga agccggggac
     9661 catcctgtgc atgccgacga cgccgttcgc cgccccgcgt cgcgggctcc ccatcaccga
     9721 actcgagccc atccgtgagc gcatcctctg cctctgcgcg catggcggtc tcgccggttt
     9781 cccacaggtc tccattcccg gggcgaccgt tgacggcgcg ccgatcggcc tttcaattct
     9841 cggcagccgc ggctccgacg cgagcctcgt cgccatcgcc agtgccatgg agggcgccac
     9901 atgaccacac cccgagacct cacccccaac atccccgagg tcgtcgccga gatcagtgcg
     9961 ctgttcgagc gctacgagca ggcgctgatt gacaaggacg tcgacgtgct ggacgccacg
    10021 ttctgggaga gcccgcacac gatccgctat gcgctccacg agaacggcta cggattcaac
    10081 gagattcacg cccatcgcgt ggcgcgtccg cccggccccg gcatcaagga gcggcgcatc
    10141 cgcctggaga tcctcaccct cggccgcgat ttcgcgacga cgaacctcga gttcaaggtg
    10201 cgcggccgcg ctctcatcgg ccgccagagc cagacgctcg tgcgcttcct gggcctcggc
    10261 tggaaggtcg tcgcggccca cgtctcgacc atgaacgaga cggcgttgtg gtgagggggc
    10321 gggcggagaa ccctagctcg acttggcacc tgcgctcttg cggtcaagca acgcgtctgt
    10381 cggttgccag gacatggtca tgccgcagag ttggcgcgcc agataccact cgcattcggg
    10441 gccatgtggc ttggtgcacg caactcggtg aagcagcgct cggcctttcg ttctcgcgcg
    10501 tgacggccgc gtcatcaggc cgagataaaa gtagagactc tgtaattcgt cgggatgccg
    10561 gaaggtgctc atatcggtta ttcgaatagt tccttcagga cgcgtctcaa gctcgaacag
    10621 atcatcgaca ccgagaacgc ttttggagaa ttcctcgggc gcgatttcac cgagaaccaa
    10681 gcgcgccaac ggttgtggcc agtgtccgtt cctccatttg gcaccaaagc gaagatcggt
    10741 ttccgctttc gacgccagct tggtatcgcg catttcgcgc gccgcgaacc acatcagcag
    10801 gccgatcgtg aagccggcac ggaggccagc gtagcaggca acgtgcctgc gatgactttt
    10861 ctccatggcg acggtcagac attcgacggc cgcgttgcga tccccctcga gccaatgggc
    10921 aacgaatttc agcccaaata gatcgaggtt gacatactcg acgtcacgag ctgtgcgagc
    10981 aaaaccttca attgcttggg cgaagctgtg ctgtgcgtcc gagtagcggt cgagccaaag
    11041 ctggtaaacg ccgagccaca tgacgtggcg gtcgtctcgc agcattgaag gatgtttgtc
    11101 gaaccagcgc accatcgccg ctggaccgac cttgtcgacg tcaggcggcc atagcttctt
    11161 gcccatgcga cgaactcact ccaactgagc gtcccctcta ctcactcacc gcatattccg
    11221 gtcacaccgg cgcgagactg gatatccggg aaacaacagc agatgtgctg gtgcgacgcg
    11281 ccccactctg cctcaagaat cacgcaccca gcgccaccgt cgccgagatc tcgaggcgga
    11341 gttcggggcg gttgagggcg gagacgatga cgagcgtgtt cggcggccat ttgccgtttg
    11401 gataaaggcg ggtgtagacg gcggtccgag ccttgaacca ggcgtcgagg ttctcgcggc
    11461 caacgacgta ggtcctgagc tcgatgacat cgtcgaagct tgcgcggaga tcggcgagga
    11521 tgccttcgag gtttgcgaac acgacgggca cctgggcagc caagtcgttc ggcccgacga
    11581 tcaaccctgc gcggtcgacc gcgacctggc ctgcgatgta ggccgtgcgc gtgccaggtt
    11641 cgacgatcga tacgtgcgag tacattccga agacaggccc cgcgctcggc gggttgaggc
    11701 gtgagacttt ggcgttcgac atcgggcgct cctcgcatgc ggctatcgcg ctacgatggc
    11761 acggcttggc agcggatggg agccacatgt gggacatggc gcggcttgat ctcgacgctt
    11821 gaaactcgcg ggctggaagc gcaacatcgt ctgcaacgat gaacgctgga ggagccacgg
    11881 catgacggcg tatccgacga aaagcctcgc ggcgcatccg ccgttcaagt atccggccta
    11941 caagggcacg atcgatcgcg cgcccacgcg gccgctcgtc ataatcccac agaccatgac
    12001 cgagctcacc gggccgatgt acggccacga gagcgtgaag gccggcgaca gcgatctcac
    12061 ccgccagcat gcgggggatg cgatcgggga gcgcatcatc gtctcgggcc gcgtgctcga
    12121 cgagagcggc cggccggtgc cgaacacgct gctcgaggtc tggcagtgca atgccgcggg
    12181 ccgctaccgc cacagcggcg agcagcatga tgcgccgctc gacccgaatt tctcgggcgc
    12241 cggccgcgcc gtcacggatg ccgaggggcg ctatcggttc acgaccatca agccgggcgc
    12301 ctacccctgg ccgaacgacc gcaattcgtg gcggcccgcg cacattcatt tctctctctt
    12361 cggtccgacg atcctctcgc gcctcatcac gcagatgtat ttcccgggcg atccgctgct
    12421 gccgctcgac acgatcttca tgagtgcgcc ggaaggcgcc cgcgaccggc tgatctcgcg
    12481 cttcgatatc gagacgacgc agccgaactg ggcgctcggc taccagttcg acatcgtgct
    12541 gcgcggtcgc gccgagacgc cgacggaggc tccgcaccat gactgatgtc tcgccctcga
    12601 tgacgatcgg tccgttcttc catttcgcca tgaccgaggg ttcgcgcggt gacggcgaca
    12661 tcgcgaccgg caacatgctg acacctgatg cggccggcga gcgcatccgc atcgtcggcc
    12721 gcgtcatcga cggcaagaat ctgccgctga cgggcgcgct gatcgaactc tggcaggctg
    12781 atggccaggg ccgttatgct gctccgggcg atccgcggcg cgcttccaac agctcgttcc
    12841 gtggcttcgg gcgctgcccg acggacgatg ccgggaactt cgtattcgat accgtgcggc
    12901 cgggtgccgt ccccggtcct ggcggtaagg cgcaggctcc acatatcgcg gtgatccttc
    12961 atgggcgcgg gctcctgcgc aatgcagtga cacgcatcta tttcccggac gaggccgcaa
    13021 atgcgaccga ccacgtcctc tcgcttgtgc cctcagaggc gcgcagcatc ctgatcgcca
    13081 ggcgcgaggc ggaccagggc ggcgtcaagg tctaccgctt cgacatcgag ctgcagggcc
    13141 cgcgcgccat gcagttcttc gaggtttgag gcgtggcacg ccgaacggag gtcggcatcg
    13201 tcggcgccgg cccggcgggg ctgatgcttg cccatctcct cgcacgcgag ggcatctcca
    13261 gcgtcattgt cgaggcgcgc tcgcggagct acgtcgaggg gcgcatccgg gccggcgtgc
    13321 tcgagcattg ggtcgccgat ctcctcgccg aaacaggcgt cggggtgcgc atgcagcggg
    13381 aagggctgct ccatcacggg atcgagctgc gctttgccgg ccgggcccac cgcctcgacc
    13441 tttccgagct gaccagcggc cgggcgatca ccgtctacgg ccagcacgag gtggtgaagg
    13501 acctgatcgc ggcccgtctc gctgccggac aggagatcgt tttcgaagcg gcggacgtgc
    13561 aggtgcatgg gatcggcgga gatgcaccga gcctcactta tgcatgggac ggcaggacgg
    13621 agcagatcga ctgcgacttg atcgcaggct gcgacggctt ccacggcgtc tgccgcgcca
    13681 gcatcccggc agagaagctc gcgatcttcg agcgtgtcta cccgttcgcc tggctcggca
    13741 tcctcgccca gtcccggccc gtctcggagg agctcatcta cgcgagccac gatgacggct
    13801 tcgcgctttt ctcgatgcgc gcgccgacag tcacgcgcct ctacctgcag tgccggccgg
    13861 acgaggacct cgccgcctgg agcgacgagc gcatctgggg cgagctcgag cgccggctcg
    13921 gcggcgcttc tggtgcgctc gagcgcgggc ctgtgcttca gcgaggcgtc acgggcatgc
    13981 gctcgttcgt ggtcgagccg atgcagtacg gccggctcta tctcgcggga gatgccgcgc
    14041 acatcgtgcc gccgaccggg gccaagggaa tgaatctcgc catggccgat gtccgcgtgc
    14101 tttcagctgc aatgcgcgac ttcctgaagt cgggcagccg gcgcttgctc gacgcttact
    14161 cagcgaggtg cttgaagcgc atctggcgcg ccgagcgctt ctcgtggtgg atgacgtcgc
    14221 tgcttcaccg cttcccggac gaagatgcct tcggctatcg catgcgcatg gcggagctcg
    14281 actacatcac gtcttccaag gcggcggcta cggcactcgc cgagaactac gtggggctcg
    14341 ccttcgaggc gccggaaggg taagcaccgg gggctgcaac ggagcaagcc ccatgccgaa
    14401 gccgatcgcg ccaaagccgc tcgactacct gccgcccgag caggccttcc tcgatccgct
    14461 ccgcgacgat ccgccggcaa aggcgcgtgc ggtcgtcatc ccgttcggca tcgaggcgtc
    14521 tgtcagctac ggctcnnnnn nnnnnggaac ggcgcggggg ccagctgcga tcctcgccgc
    14581 cagtcaccag ctcgagctct ttgacgagga actttggcgc gagccctggc gcgacttcgg
    14641 catcgcgaca ctgcgagagc cggtgatcga caagtcggtg tcgcgcgcac tcgatcagct
    14701 cgagactatc gtcgagagcg ttgttgcagc aggacgcttt ccgctcgtgc ttggtggcga
    14761 gcattcgctc acgcctggcg ccatccgtcc gctggtgaag cgccacgagg acctcgtggt
    14821 cctgcaactc gacgcccacg cggacctgcg cgacggctac ctcggcgaga cattctcgca
    14881 tgccgccgcc atgcgccgcg tgctcgatca tccgggtgtg tcgcttgtct cggtcgggat
    14941 ccgcgtcttc tcgagcgagg aggcggcatt cctcgagggg gcaggggagc gcgtgcgcat
    15001 ccactggggg aaggaccagg cgcgctggga catcgaggag atcgtaaggc cgcttcgggg
    15061 aaagcgggtc tacgtcacct tcgatatcga cgcgctcgat tcggcggtga tgccggcgac
    15121 cgggacaccg gtgccgggcg gcgtgccgtt cttgactgcg ctcgccatat tgcggcgcgc
    15181 ggcggaggtc tcaaccatcg tcggcggaga tgtcgtcgag ctcgcgccga tcgcgggcct
    15241 gcacgcctgc gactacacgg cggcggcagt cgcctcgaag atcatgacgt atgcgctctc
    15301 cgggacaggc gtacgggcct gcccgccgcg ccgttaacgc gccctttgcc aagctcgcct
    15361 agggtcgaca ccgggtggca tatctcatgc cgcccgcccg gaggcccgca tggtgtcgcc
    15421 cgtcgaggtg tcgatcatcg ttgtcagcta caacacgaaa gcgatgacgc tggcgtgcct
    15481 cgcctccgtg cgcgccgaga cgcgcgcgtc cttcgagctg atcgtcgtcg acaacgcatc
    15541 gagcgacggc tcggccgagg cgatcgccgc ggctgacccg gaggccgtga tcatcccgtc
    15601 gcgagagaat ctcggctttg cgcgggcgaa caacctcgcc gcgcgccgtg cccgcggccg
    15661 ctacatcctt ctgctcaatc cggatacgct cgttcacgag ggcgcgatcg acaggctctt
    15721 ggcctttgcc aaggcgcggc ccgcggctcg catctggggc gggaggacga ccttcggcga
    15781 cggacggctc aatccggcct cggcctgggg cgagatgacg gtctggaacc tcttctgcag
    15841 ggccgctggg ctcaccggtc tctttccgag gacaacgctc ttcaacggcg aggcactcgg
    15901 cggctgggac cgcgcgagcg agcgcgacgt tgatatcgtt tcgggctgct tcttcctgat
    15961 cgagcgcgcc ttctggcagc aactcggcgg cttcgattcg gctttcttca tgtacggcga
    16021 ggaggcggac ctgtgcctgc gcgcccgcga acttggggcg cgtccccgcg tcacgccgga
    16081 ggcgaccatc gtccactacg gcggtgcctc ggagacggcg cgcgaggcga agctcgtcaa
    16141 gctgcttgcc gccaagagca tgctgatccg ccggcactgg ccgcgcggcc ggcgcctcgt
    16201 cggcctcgct ctgctctccg cctggccgct gacacgggcc ctggcaacgg cgattgccat
    16261 cccgttgaac gccggcgcct ggcgggcctg ccgcgagtgg cgcgccgtat ggcgtgcgcg
    16321 tgcccgctgg ctcaatggat ttccgatcat gacctcgcaa gcctcgttgc gtgtcacggc
    16381 ccgacccgga acgggaggag cgccatgacc acggccagcg cagtcacaat ggttcgcccc
    16441 gccagcgagg gacccgtccg cttcacattc ggcacgctga tgacgtcgcc ggagcagcat
    16501 gccgcgctgc tcgccacgct cgatgcccac ggcttcggcg ccgacgactg cgaggtgctc
    16561 gtcgtcgaca acgcgagagg tgcgctccgc gatgcctatc gtggcctcaa tgagatgctg
    16621 aacgcggccc gcggcgatat catcattctc tgccatcagg acgtgcgtct tctcaaggat
    16681 ggacgcgaag agctcgaggc acgcctcgcc gacctcgagg cgaaagcgcc cgattggggc
    16741 gtcgccggca atgccggtgg catcagtccg ggccggctcg cactccgcat taccgatccg
    16801 catggcgccg atctccaagt cggcgcgttt cccgcgcgcg tacagtcact cgacgagaat
    16861 ttcctgatcg tgaggcgcgc ggcccgcctg tccttctcgc gcgatctcac cggctaccat
    16921 atgtacggct ccgacatctg cctcgtcgcc gatgtgctcg gatggtcgtg ctgggtgatc
    16981 gactttcaca tcgagcacct gagcggcggc cgcaaggacg agagcttcca tgccgctgag
    17041 cgcgagttca acgccaagtg gcgtcgggcg ttccggccgc gcctcctgca gacgacgtgc
    17101 acgctcgtct ccattgcggg cgatccggtg ctcggcttct tcgggcgcat gggggttggc
    17161 ccgatcggca agatttcgcg tcggctgcct ggcgcgcaag ggttcggtgc tcgccgggac
    17221 ggccaatgac ggccgcgcac caggccgcgc gcatgccgtc tgcaggcgtc cgccgtcggg
    17281 tccttgggca agggcttctt ctcttcgccg ggttcggcgc gtcgcaagcg atgtcgctcg
    17341 ggcgcaatgc gctgctcggc tacctgctgg cgcgcggcga tttcgggata gccgcatcga
    17401 tcactctgac gctgcagact gtcgagatcc tgaccgacct cgcgcctgat cgcttcctca
    17461 tgcaggcgcg agccggcgag gaggcgcaca tgctcggcgc cgcacacgcg atgtcgctcg
    17521 cccggggcac gctgatcgcg ctccttctgg ttctcgccgc tgagtcgctc gcgcacttct
    17581 tcggcgtgcc cgaggcgcgc accgccttca tgctgatggc gctgacgcca ctcatcaagg
    17641 gggcgctcca cctcgatcat cgcctcgccc agaagcgtct cgacaaccgg gcttatctcg
    17701 cgacggaggt cggcgggcag gcagcggcgt tcatcgtggc ggcgccgcta ctcctcgcct
    17761 atccgctgcc gatagtggtc gtctggctgg cgatcctgca ggccgtggtc gccgtgggca
    17821 tatcgcatgt ggttgccgag cagcccttcc gtcttgtcct cgaccgcggg gtgctgagga
    17881 gcgctctcgc cttcgggtgg ccgatctggc tctcggcctt tcccctgatt gcggtctatc
    17941 aggcggaccg gctgattgtc ggccgcgtgc tcggcatgga ggcgctcgcg gcctactcgg
    18001 cggcagccat gattgccatg gtgcctgggc tgatcgccgc caaggtcgga caggcgatcg
    18061 tcttgccgat gctctcacag cgggcggatg acggggtcgg cttcggccgg gcctaccggc
    18121 tcatgctcga ggcgagcgtg attgcggcgg cgggctacct cgccgtctac gccatggccg
    18181 ggggcacgct cctggcgctg gcgttcggtc cacagtatcg ggggctcgac ctgctcgcgg
    18241 cgtggtgcgg ggcgatgtgg gcactccgca tggtgcaggc gattccaggc atggcactga
    18301 tggcgcaggg gacgacgcgg ccgctactga ttgcgggcgt cgtgcgcgcc tctgtggtgc
    18361 cgctggtggc cgtcgtcgcg gtgatgcgct tgcccctcga gatgattgcc gtggtgggcc
    18421 tcgttggcga gctggcctcg ctggcggtgc ttgccatggc gctcgagcgg acagctgtcg
    18481 gcgcgggtct ccgccgctcg acgctcctcc gaatcgccat gctgacgccg gtgacgggcc
    18541 tcctggcact cgccggtcat ggcctcccgc tcgcgagcac gattgccgtc gcagcacttg
    18601 ccgtgctcgc cgccgcattc ctgattgcag gcctgatgcc gggcatccgc gccatggcgt
    18661 gggcttcgat caatggcatg gacaacagtc gtgcggcaga cgagccgcct caattctacc
    18721 caaccgctca ccgaaacggt taaggcggaa ttaaccggtc tgcccttacc gctgtcatct
    18781 gtcctgccga tcatcggcgg ttcgcaggtt ctcaaatgcg tcccgacgcc accacgtgcc
    18841 aatcgctgcg cggcctcgcc atccgggcga tgctcctcgc gttgtttgca acaggactgg
    18901 tgccgaccgg gccagccctt gctgcgcggc ccggagaggg cgagatcgtc gcgccggcac
    18961 cccgtccggc gatccggccg ccgcggccgg gggctccgag caacgctgtc gcgctcgccg
    19021 agcgcctgaa gcggatcggc gccgtcaaga gctggggcta tcagctcaat gggctcaatc
    19081 tcgaggccgc cgccgcttcg ccctatgatc tgctgatcgt cgatgcgacg accgggctcg
    19141 cctcgggccg gcccttcgag gcagccgagg tcgagcggct gaaacgcaag cccgatggct
    19201 cgcggcggct cgtagtctcg tatctctcga tcggcgaggc ggaggactac agacccgagt
    19261 acttcagccc cgagtacatg agcgaggatg cacccgactg gctgatgcac gagaatccgc
    19321 agtggaaggg caatcgcatc atccgctttt gcaacgaagg ctggcagctc acgatcctcg
    19381 gcgacgagcg tggccgcagc gtctacaaca gcatcgatcc ctcgccactc tttcgcctga
    19441 tcgagctcgg cttcgacggc gtctacctcg accgcgtcga cgtctacaag gaggttgcca
    19501 aggagtgcgg cgaggccgaa cggcgcatgg tggacttcgt ctcgcggctc gcgcgtcacg
    19561 cgcggcgcaa ggagccgcac ttcctcgtca tcatgcagaa cgccgaggaa ctcgcccgcc
    19621 aggcgaagct catcgaggcg atcgacgcgg tggcaaagga ggacctcttc tacggcgcgg
    19681 atcactctga gcgccagaac gaggcgggga tggtgcgcac cgttctcgac aacctgcgcc
    19741 gcatcaaggc tgcgaaccga cccgtattcg tggtcgacta tctgaaggac cgcacgaaga
    19801 tcgacgatgc gaagcgccgc atcctcgatg cggggttcgt gccctacatc gcgccgcgcg
    19861 ccctcgatcg cctctggctg cccggacgcg atttctgagc tgatcggcag cgggcactat
    19921 tgcccgctgg accgctcctc gcttgccccg gcaactccct cgctgttgac cgtgatctcc
    19981 cagcgtgacg ccggcagccg ggcgcgccgc aattcggccg tcagcagagg accgcgatag
    20041 ttccgcgtgc cgtcaggcag gcgggcgatg agttcggggc acgcctccgc gctcgtcgta
    20101 ccggttgtgc gatcgatcac gagggtgacg cagtccgagc cgagcacgga ggtctcagtc
    20161 gctggtccgt gtgacagcag ccctgcgagg aaagcccaga agccgaccgc gtgccagaac
    20221 acggcgcctg agaggaaccc cattgtcgcc cagccgacgt tcgaccagcg gttgccggta
    20281 tcaaatatgc cgtcgtcatc gatgtcggca acgcttgcgt ccgctgatcc gctcaccgga
    20341 tcgtgcgcac cgtgctcgtt tgcggcagcc tcggagggcg ccgcggaccc gtatgagcgc
    20401 atggcagccc ccgctgttgc ctcatttccc acgaaaacat tgcttgatcc gcgtgttatc
    20461 aacaatgctc gccgggcctc accggggcct cgtggcgact ttatccactt gaaaatgacg
    20521 cgccttctac aacctgccat tgtttggccg tggttgcaga tgggtccgat ctaggagcag
    20581 tgacatgtcg agcatcgaca tccccacgac cggcgaggca aggatcatcg gccccgagga
    20641 cggggcgagc ttttggcagc cggtgccggc gaacggcttt gtgcgcaata tcctcaatca
    20701 ggcgatggtc ggtgggaacg cgagcttctc gatgggcacg cagaccgtgg cgcccggctg
    20761 cttcatccgc gagcacacgc acgcccgcga ggaggagatc atccacgtgc tggagggtga
    20821 ggggatcgcc cgggtggagg gcatcgagac gccgatctcg cgcggctcga cggttttcat
    20881 cggctgcaac cgccgccacc atttcctcaa tcgcagcgcg agtgccccgt tcacgtttct
    20941 ctggctgatg ctgccgggcg gcctcgaccg attcttcgcc gagatcggcc ggccgaggac
    21001 gcccggcgat ccgacaccgg cgccattccc gcgtcccgac aacattgccg agatcgagcg
    21061 gcgcacggtg ttcggctgga ccgacaagag ctacgacaaa tcgtagactc agacggtgcc
    21121 cggaagcttg aagaagctcg tgccgaccaa gatcatgttc tcctgagaaa tgagcgctcc
    21181 gagctcaccc gacttcttca cgtaggcgag ccagtcggga tcagcccaca tcgcggcgcg
    21241 cctggcggtc cggtccgcga ggtccttgta ggcccacacg tgaatgtagg cgttgacgtc
    21301 gccgacctcg gtcgtcgcgt agagcactgg cttgccgagg tgcttctgct gggtcgcgaa
    21361 gccgtgcttc tcgtagagct cgagatgcgt cttgatcatg ccgggcttca cgcgataggt
    21421 gcggtggtcg aagatcatgg ggtgtttcct cgttgagtgc tgtacgcggg cgagcgtagc
    21481 gtgcgcgccg tctcacatca cgccaaactt ccggaaggcc tccgcggcgg gcataccggc
    21541 cctgatcgcg tcgccgacct tgttctcggt ctcgaccttc tcgagcgcgc gggcgataca
    21601 ttcctcctcg gcctcgcgcg ggatcacgat cacgccttcc cggtcaccgt agaggagggt
    21661 tcccggctcg atgcggacgc cagcgatttc gatcgccgag cggaagtcga tcaccttgcc
    21721 gcgcggtccc tggtcctggg cgtagaggcc gcgcgcgaat accggaaacc cgatcgcctc
    21781 gatctgcgcg ccgtcgcgca catagccgtc gagcagtgcg cccgcggcct tgaggcaagt
    21841 cgctcgcgtc gccatcagct cgccgaagag tgcgtagcgg agcgaggcac cggtcgcgac
    21901 gtaaatctca ccgggcttca ggtcgtcgag cgcctcgaac atcagcccga acggcttccg
    21961 cgcgagcggc ccctgcgagc tcggccggcc atcgtcgaag atgtcagcct cgagcacggg
    22021 catggcgcgg ccggcgaggc gcatcgccgg gttgagcggg gcgaagcccg ccgggaggaa
    22081 ctggtggcga aatcccatct ggtcgaggac gtcgccgacg accgcggtat ggagcctgcc
    22141 gttcgtgagc gtttcgagga tatcggtggc cttcttgcgc ggcggcatgt caaactccgt
    22201 tcgagcattg cgggagacag agtctttcag gagagcgcga gacccgcaag cttttcgccg
    22261 cgcagcgcaa cgacaagcaa cgggaacgcg ccttggatca tgtcgctgtc gccgccgatc
    22321 gtgtccgagg ccgggccctg cgcgttgtcc tccccgaaag caccgatgag cgtatcgtcg
    22381 aggcggcacg gcgcctcgcc gccgaggact tggcgacacc catcctgatt gccgagtccg
    22441 ccgaggctct ctcctcagcg ccgttgacat cgatccatcc accgaccgcg ccctcgctct
    22501 ccaggtacgc cgccgcactc gccgcacggc gctccgccac aggacgggcg atgacggatg
    22561 cgatggcgag gcgcctcgtc tcgaagccgc tctacttcgc aggagcaatg gtcgctgccg
    22621 gcgatgccga ggcggtcgtg gccggcgtcg caaatccgac aaggcgggtg atcgaggccg
    22681 gcctgatgac gatcggcctc gccgagggta tcgccacgcc gtcgagctac ttcctgatgc
    22741 gtgtgccagc acgagctgac cgccccgccc gcgcgatcat cttcgccgac tgcgccgtcg
    22801 tcgccaatcc cacaagcgag gaactcgccg atatagcgat cgcgaccgcc ggcagtgcgg
    22861 cccggttgct cggcgagacg ccgcgggtcg cactcctctc attctcgacg catggcagcg
    22921 ccacgcatcc cgatgttgcc aaggtcacct cggcgctctc gttgatccgg gcacgcgcgc
    22981 gtgcacttgc cgtcgatggc gagctgcagg gtgatgccgc gctctcggcg gcggtcgcga
    23041 ccaaaaaggt caaggcggaa agcgccgtcg ccggtcgcgc caacatcctc gtcttcccag
    23101 atctcgatgc cggcaacatc gcctacaagc tcgtccagga gcttgccggc gcggaggcgc
    23161 tcggcccctt cctgcaaggc ttcgcgcggc cgatctcgga cctctcgcgt ggcgcctccg
    23221 tcgacgacat cgtgcgcaca tgtgtgatca cgctcgccca agcggccgtc tcatgacgcg
    23281 gcgagcagcc agagcccgcg tgcgatgaag gtgaggctca cagccaggat gatgcgccgc
    23341 gcccagcggc ggaaatcggc gtccgtcatg gcctcgagca cgcgcgcggc gagcgtcgtg
    23401 ccgacgatcg ccgggatcac cgcgccggca tagacccacc agggtatggt gacgtcgaag
    23461 gccgtcatga gcgagccgaa gtagaagatg cgcagcagat gcgcgaaggt ctgcgtcacg
    23521 gccttcgtcg cgacgatcga tttgcggtca agcgcgctct tctggaagaa aacgtcgagg
    23581 agcgtgccgg tcgcacccgc caacagattg agcaccgtca ggattgcgcc acagatgatg
    23641 ggcatgccgg gacgcgtgat gtccggctcg agccgtttcg gcagcagatc ggcgaggaag
    23701 ggcatcgagc cgagtgccag gtacatgaag gccttgctcg ggacgtaggc gatgaggcgc
    23761 atcacgagga atgccgccaa cgctccgagg cagtagccgg cgacgacccg ccaatagatg
    23821 tggtcctgcc agagtgtcgc ccgccagccg ttggcggcca tctgcgtcga tccgaacagc
    23881 accatggcct ggccgacatc gaggacgagc atcagcacac cgagcaggat gatgccgccg
    23941 gccatgccga agatgccgga gagaaacgag gtcgcgagta cggcgagcgc caggagggtg
    24001 agcgtcgcgg gggcgatcat cgtgcggtcg cgatcacgag agagggctgc tgcaactcag
    24061 gagatccgta accatcatcc gtcagagtgc cgtaaaagtg gcgcgtggag catttcatgt
    24121 ccgaggtccg ggcgttcgac ggcatcatct gcattggcgg cgaggactgg tggtaccaca
    24181 accgcggcca cttcgacttc cagatcatgc ggcggctcgc ccgctccgtg ccggtgctgt
    24241 tcgtgaactc gctcggtgtg cgcatgccct ctgtcggcga gagccggctt ttcctcgctc
    24301 gcatcggccg caagctcgct agcctgaagc gcggcctcgt ggaggcggag cctgggttct
    24361 gggtgttctc gccgctctcg gttccgggtg ccgcgggcgc gaagctctcc ggctgggcgc
    24421 ttgggcccca aatcaggctg gcggcgcggc gcgccggcat atcgaggccg ctcctttggg
    24481 tgcactgtcc ggctggcgcc tcgctcgtcg gccagatccc ggcggccggc gtggtgctgc
    24541 agcggacgga ccgattcgag gcatttccgg aaggtgaccg cgcgttgctc gccacccaga
    24601 tcgcgagcct gaaggccaca gccgacctcg tcgtctatgc agccccccat ctgatggcgg
    24661 aggagaccgg gacggtcgcg cggcaattgc tcgtcactca cggcgtcgat ctcgagacgt
    24721 tcgtttcggc cggacgcaat gcagcggatg ccgggcctgc ggacatggca cacctcccga
    24781 ggccacgcat cggcttcatc ggcggtatcg acgcgcacac cttcgatccg ccactgttcc
    24841 tggcgctcgc agctcgcatg ccggatgttc gtttcgtgct cgtcggcggc acgtcgctcc
    24901 cggagggctg gtgcccgctc gaaaacgtga cccaactcgg ccgcaagccc tacgccgaag
    24961 tggcccgcta catggcggcc atggattgcc tcatcatgcc atggaacgac agcccctgga
    25021 tccaggcctg caatccggtg aaactcaagg agtacctggc gaccggccgg ccggtcgtca
    25081 cgcgcgactt cgccgccctc gacggctggc gtgatctcgt ccggacggcg gcgacaccgg
    25141 atgcattcga ggcggcggtc cgcgcaagcc tcaccgaacg ccgcgacatc gaggcgattg
    25201 ccgctcggct cgcgtccgag acctgggacg ccaaggccgc gctcgtgctc gatgcgctca
    25261 ccgatgccgc cgccgcaaag tcgcagggaa cgcacgagcc gaaggttgcc tgatcagatc
    25321 aagacgaggc ccgaagactc acgagtgtga caccggagcc aagctcgcct gcgaggactt
    25381 cgagcgcgga gcgcgaccac gccacatcga tcgccacggc cgtcagccgg gcggacccga
    25441 ttgccgccgc cagggccgcg atatcgggtt cgcgcggttc gtctagacga atgaccacca
    25501 cgccgtcgcg gcgcgccgag gagcggtcgc cccactgccg cagggcacgc acgcgcgcga
    25561 gcatggcaag tggtccttcg gccgccgata ccgcgaggac atgacgccgc gccactgcaa
    25621 ccgctgctcc ggcgttgaaa gcagcgaggc ggcgcgagac ctcggcggcg atctgcaact
    25681 cgcgccgccg tgccgcccgg aggcgacctg cgacttcgtc gacgttcaat tcccgaacct
    25741 gcctgtccgg gctagccgcg ggctcgtcga gaccggcaat ggcaacacgg ccatcatccg
    25801 ctgccgcgat gaggcgctcg cgttcgagca gcagtgcgcc acccggcgca agcgcgatgc
    25861 ccgacagctg cgcggcgatt gcgccggcga tggttggcgg tccgattgct gggaggtcga
    25921 tgcgcagctc ctggccgggc ttcgggcgct tgacgaggac gccggacggc gaggcgtcgg
    25981 gtgcggcaag tatcgccgcc cgggcgagca tcctgtcggt cccttccgcc gcctcgatgg
    26041 cgatcacctt gccgccccgc acgacaacgg cctgcccgac atccagcggg ccgatggcac
    26101 cgatgaggcg tgcgccgagt gcagcgtcgg cccggtcgga ggccctgaat tcatgcctgc
    26161 caatgctgcc ggcgcgcgca acgagctccg gcgcgaccgc cggcacgccg agcacggtga
    26221 agccctgccg ctcgaagaag cgcaggatgc gcgtcaggat cgcatcatcg ccatcgcgaa
    26281 ggatcgagaa gaacatcggc agatgtcgaa agaagccgag gtcggggcgg agtgagcgca
    26341 cttcgggccg cgtgatccgg ccgacgaaaa tgatctcccg gcagcacgcg gcccggagcg
    26401 cgccgagcag ccggccgacc gcagctatgc gtgtccaggt gtgcgggtag gcctcgacct
    26461 cgcagcctgc cgagccctcg atggcgacga tatgcaccgc ccggccgcgc cgcgacaggc
    26521 tctcggcgac ctcgatcggc aacgagccgc cgccggcaac gatgccgacc gggcccatca
    26581 tgtcaggcat cggatccggc gccaggggta cagagggaac gttttcccgt gacgcgcatg
    26641 aaggcgatga tctcctgcac gatcggatta tcgccgtact cctgcgcgac gtcggcgacg
    26701 cgctcctgca gcgtgccctc ggccgcgaac agcaggcgat aggcgcggcg cagcgcatgc
    26761 acctgctcgc gcgtgaagcc gcggcggttg aggccgatga tgttgaggcc ggcgaggtgc
    26821 gcgcggttgc cgagcgccat gccgtagggg atcaggtcgt tctcgaggcc cgacatgccg
    26881 ccgaggaagg cgtgagggcc gacacgggtg aactggatca ccgcggcgcc gccgccgagg
    26941 atcacgtagt cgccgatcgt gcagtgtccg gcgagcatga cgttgttcga gaagatcacg
    27001 ttgctgccga cgtggcagtc gtgaccgacg tggctattgg cgaggaaggc gcacttgtcg
    27061 ccgacggtgg tcgccgagcc gccgcccgcg gtcccggggt tcatcgtcac gccttcgcgg
    27121 atgatgcagt cggcaccgat cgcgagcgtc accggctcgc ccttgaattt cagatcctgc
    27181 ggctgatggc cgatggaggc gaaagggaaa atgcgggtgc gcgcgccgat cgtcgtcttg
    27241 cctgcgacca cggcgtggct gacgagctcg acctcggggc cgagctcggc ctcgggcccc
    27301 acatggcaga agggcccgat gcgcacgtcg gtgccgagcc ttgcgccatc ctcgacgatc
    27361 gccgtcgggt ggatgcggct tgccatcagg actgtttgga caggaaggct tgcgtctcgg
    27421 gcgtgtcgac gatcatggct gagacctccg cttcggcggc gacctgccca tcgacccgcg
    27481 cctcaccata gaagcgccag gcgcggcccc ggctccgcaa cttccggacg tggtagcgca
    27541 actgatcgcc cggcaccacc ggccggcgga actttgcctt gtcgatcccc atgaaataca
    27601 cgagctgcgg cttgtactca gtgccgaggt tgtgaatgca gagtgcgccc gccgtctggg
    27661 cgagcccctc gatgatgagc acaccgggca tcacggggaa cgccggaaag tggccctgga
    27721 agaacggctc attgctcgac acgttcttga tgccgacgca gctctcgtcg cggtccatgt
    27781 cgacgatgcg gtcgacgagc agcatcgggt agcgatgcgg cagcaattgc atcacgcgca
    27841 tgatgtcggc atggccgagg ccgggcttcg gggttgcatc actcatgagc gtctccagtc
    27901 gtcattcaga agaggcttac ttcggcagtc atcgtcggcg tctctcgagg cagccgcctc
    27961 aggcggcgtc gccctcgtcg tctgcgcccg cggcagccct gcccttgcgg gcgagctggt
    28021 tgaggatcgc cacctcgcgc gcaaagatct taaacggccg cgcgggaatt ccgccccacc
    28081 gctcgcctgc cggcacattg ttgtgcacgc ccgccttgcc cgcgatctgc gctccgctgc
    28141 cgatcttcag atgcccggcg atcgccgtct gaccaccgat caccacgaaa tcaccaagct
    28201 cggtcgaccc tgcgatcccg cattgcccga cgatcacgca atgtcgcccg ataaccacat
    28261 tgtgcccgac ctgcactaga ttatcaattt tagttccctc gccgatgatt gtatccttaa
    28321 gagcgccgcg atcgaccgcg gaattggcgc cgatctcgac gtcgtcctgg atgatcacgc
    28381 ggccgatctg cggcactttc aggtgcccgc gcggacccat cgcgaagccg aacccgtcct
    28441 gcccgatacg cacgccggca tggatgatca cgcgatcgcc aaccaggccg tgtgtgatcg
    28501 atgcgcccgg ccccacataa ccgtcgcggc cgatgtgtac ccggtagccg accacggcgc
    28561 cggcggcgat cgtcgtgccg cggccgatgc gcgcctcgcg cccgatcacg gcgcccggct
    28621 cgatgacggc gcccggctcg atctcggcac tcggcgagat tgtccgcccc ccctcacgct
    28681 gctcttcgag cgcggccttc gacttcatcg cctcgggata gaagagctgt aaagcgagcg
    28741 cgaagccttg atacggagca cgcgtcgaca gtttgatgac gtgggctggg acacgtcctg
    28801 cgagcgccgg cgcgacaagg caggcccgcg ccttggtcgt ggcgagctga gcagcataac
    28861 ggcggttgtc aatgaaggag agatctgtcg gaccggcatc cgagagcgag cggacgtcag
    28921 tgatcagcgc tgcggcgtca tcggtgccgg aaagctccgc ccccgtacgc ttggcaacat
    28981 cggtcaacga gaacggaccc gcgcatttga agaacccagg atgctgcatc gccaatccgt
    29041 tcccgctctc ggtctcatga ggctgcacga atacgccacc tcgctgccga caacaaccgg
    29101 gaagccgccc gccgcgggaa gcgcagaggc ggggccgggg gcagcctcga cacgagacgg
    29161 gcttttgcgg gggcgagctt cgctgcgcga gtgaggctcc cgcctcacgc tccggccgga
    29221 ggatatgctc cggacctccc cttcttatga cggaagattt cggttggcgt aaatcagccc
    29281 acgcgttggt gtcggcctga atattccaac ttccattcaa aaaacggagg gggtttggga
    29341 gggtctccct cccaaatggg tacgggcgat agcccgatcg cgaagcgaag ttccaatcag
    29401 ggccggactc tccggctcca aatgcgaacc gggcccctga ggggcccggt tggaaggtca
    29461 gaatttggtg ctggcgccga agcggaaaac ctgagtctgg tcgtagtctt ccttctgcaa
    29521 ggcccagccg aagtcggcgc gcagcgggcc gagtggcgag ttccagagga ggctcgcgcc
    29581 ggccgagagg cggatcgagg agtcgtcggc aaggttgacc acctgaccgg tcgctgggtc
    29641 agctgtaccg ttcaggcgct tggcaagggc cgaggcgccg aacagcgagc cggcatccac
    29701 gaagaaggcg cccgacatgc cgaggtcatc cgggacgagt ggcaggggga agcggacctc
    29761 agcggtcgct gcccagaacg tggtgccgcc gagcgcatca ttcgttgaca agtcacgcgg
    29821 gccgataccg gagcggtcga agccgcggat cgtctcgccg cccttgtaga acatgtcaat
    29881 caggcggact tcgtcaccac cccagccgaa gatgtggcca ccgacgacac ggccgaccag
    29941 cgtgatcttc tccgtgatcg ggtagtagcc gcggccctcg ccagtgaagc ggacgtactg
    30001 cacgtcaccg ccggcacccg cgacgtccga gcttagcgtc aggtagtagc cactggtcgg
    30061 cgagcgcgga tggttccgcg catcataggc gatcgaggtt ccaaccgacg acgtgatggt
    30121 gacgccctcg gcttcacgga tggcgcgcga cgcgatcacg ccgtccgagg cgtccttgtc
    30181 gacgtcgtag atctcgtcac gcgagagcgt gtagttcgtc gacatccaca ggttctcggc
    30241 gagcgggaag gcaaggcgca gcgagccgcc ggtcttgcga ttccggaagg cctggcggtt
    30301 cgactggttc acgtccttgt ggaacacgtc gaagcccgcc gccaagtttc gatcgaggaa
    30361 ccgaggctcg gtaaaactca agtcgatctg cagccgctcg atcgagcccg aaagcttcaa
    30421 gcggatgaac tggccgttgc cgaaaaggtt gcgctcggtg aggctgatgt cgccgatcac
    30481 accctcggcc gtcgagtagc cggctccgaa ggaaagttcg cccgtcggct gctcgacgag
    30541 atcaacatcg agaacgacgc gatccggtgt agagcctgcc cggcgcttga tatcgacatt
    30601 cttgaagaag ccgagcccct gcaggcgctt cttggcgcgg tcgacgagca gcggattgta
    30661 ggcgtcacct tccaccagcc ggaactcgcg gcgaatgacg tagtcctgcg tgcgcgtatt
    30721 gccgttgacg ttgatgcgtt cgatgtagat ccgcgggcct tcgtcgatca cgtatacgag
    30781 attgatcacg cgtgcagcgg cgtcccgatc ggcgcgcggc cggacacggg cgaaggcgaa
    30841 gccctgctcg gaaatggcga gggtcaagcg ttcgactgac ttatccatca gcgaggcatt
    30901 gaacacctgg ccgcttttgg tgagcaactg gccggagagg gacggtgcat tgatggccgg
    30961 caggctgctc tcgatgctga ccccgccgaa gcgataaagc tcgccctcct cgacgacgaa
    31021 ggtgatgaag aagccggagc cgtcgcgatc gagttcggcg ccgccggtcg agatgcggat
    31081 gtcggcgtag ccgttcttca aatagtattg acggagcagc tcgcgatcgt aagcgaggcg
    31141 gtccggatcg tagatgctat tccccttgat gaagtcgaag aggccgacct gtgtcgtcga
    31201 gatgatgtcg cgcagctggc tgtcggagaa tgcccgattg ccgatgaagt tgatggcctt
    31261 gaccttggtc gcaccgcctt cgttgatctc gaagaccaag ttgacgcggt tatgctcgag
    31321 ctcgatgatc ttgggctcga cggttgcggc gaagcgtccc tgacggcggt agagatcgag
    31381 gatgcgctgg acgtcggcct ggacacgggc gcgagtgaag acggaacgcg acttgagctg
    31441 cacctcggac tgcagcgtcg ccgtgtcgat ctcgcggtta ccctcgaagg cgacctgatt
    31501 gatgatcggg ttctcggtga ccgatacggt taccgtcgcg ccggagcgat ctatgcgcac
    31561 atcggagaac aaaccggttg caaaaagcgc tcggatcgac tgatcgaccc tgccggcgtc
    31621 gtaaggatcg ccgatcgaga atttgagata ggacctgacg gtctccggct cgacacgctt
    31681 attgccgaca actctgatct cgcgaacgcg accgtcttgg gcctgcgctg tagcgacgat
    31741 cgcgccgctc ggcatcacac cgctgctgag cagtgtggcc aggagcgcga ttgcgacagc
    31801 cccccaaacc ctcgtcaatg gaaatgccgg catttgttct cgcctgcgtt cgttgcgccg
    31861 atcccccgtc tcgtcatgcc cgatcggcgt tcggtcatga cccacgagcc atacgtcgtc
    31921 ttgcgaggcc ttctcgactg ggcctctgca ttgttcggac catcgcacac cgaagtgatg
    31981 cagactgtcc ggttggaccc gacttggagc ctcgtgacgt acccctgtcc ggtcaggcct
    32041 tccgagcgac gtcttgcgaa tgtcctgttc gcgatccggc gcttccgttc gtcgtctcag
    32101 tcggtcgacc cgttttatcg acccagtcca ttgttcgtcg cagttcattg ttcgcagcga
    32161 tcaggtgggc gcatcaggac gcttcccatc tacttccgcc accttaaagg tttaacgatt
    32221 cagtcactcc tgaggaagtg gcaagaatgc ttcagtggcc cgttttttac aggccttcag
    32281 cccccgaaag tcagccactt ctggaggatt ccgcggtcgt tccacatcac gaacagcatg
    32341 agggtgagaa caacgctcgc gccgatcttg aaaccaaatt cctgcgcctg ctcgctgagg
    32401 gggcggcgca tcacggcctc ataagcatta tagagcaggt gcccgccgtc caggagcggg
    32461 atcggaaaca ggttgaaaag cccgatggcc gccgagatca aggcaagggt gtagagccag
    32521 ggaacgaagc cggccttcgc catgcgcccc gagacctcgg caatccgcac cggacctccg
    32581 agctggtccg cgctctgacg gcgcgtgacg atatccttca ggtagttgaa ggtaccgggc
    32641 acaacctccc aggtctgcca ggcaccccgt ttcaaggatt cgaagaaggg atacgaccgt
    32701 tggttgaagt cgccttccat gggggcgacg ccgatcttgc cgaccctgat cttcccaccg
    32761 aaggtgtccg cctcctcgga aaagcgcggc gtggccttta gtgtgacggg agcgccccca
    32821 cggtcgatcg tgaacgtgag ctcgtactcg gcgctctgcc cgacaatttt cacgagatca
    32881 ttgaacgtct cgatgcgcgt tccattcacg gccgtgatca cgtcgccggg cttgaaaccg
    32941 gcgctctccg cgacgctgcc cgggacgacg cgcgagatgc gggcatcctt gacccgttcg
    33001 ccgacggtca tgaggatgcc ggtaaagacg agggtcgaca ggatgaagtt ggcgatcggg
    33061 cccgccgcga caatcgccgc gcgctggccg aggggccggc cctggaacga gccctccacc
    33121 ttggtccagt cgaccgcagc gtctgtgccg gtccccgagc gcgcgggaac actcgcctcg
    33181 ttggcgtcgt cgataaactt gacgtagccg ccgagcggga tccaggcgaa gcgccagcgg
    33241 gtgccgtggc gatcgtcgaa gccaaagatc tccctgccga agcctatgga gaaggcctgc
    33301 accttcacgc cacaccagcg cgcgacgaga aagtggccga gctcgtggat gaatacgacg
    33361 atcgtgagca ggcccacgaa cgctatgaag gtcatcatga tctgcctctg atctgcaacg
    33421 tatgcgtgtg gcgctcgcgg aggcgcctca tgtggtgcat ggatggcttc gcggctcccg
    33481 gccattgggc cggatcagcg cgggctcggc atgtcggacc ctgctggaca tcatgggcgg
    33541 gagctgtcct agggctgtcg aagtcgtcat cgcaagctct ctagcgcaag gctgtggcgg
    33601 cgcgaagatg gcaaatgcgc cccgggaggt gcaactgcaa gggggggcat cgccggattc
    33661 attggccgac gagcaaggcc cgtgccggcg agtggacgtt gatcagcaca gctgcggctg
    33721 ccgcgacgac agcagcgatc accaagccgt cgacccgatc gaggaagccg ccgtgcccgg
    33781 gaatgaggtc gcttgcgtcc ttcacgttgt gcttgcgctt caaggccgat tcggcgaggt
    33841 cgccgagctg cgcgacgatg ccgagcccga gaccggtcaa cgcgaggcgc agcgctgaac
    33901 cttgctcgac gaaccgtgac aggatcgcgc ccgcgatcgc gctcgttccg acgccgccga
    33961 cgaagcctgc ccaggtcttg ttcggggaga cgcgtggcca gagcttcggc ccgccgatgg
    34021 cacggcctga gaaataggcg gcaacgtcgg tgccccaaac gcacacgaac agcagcagca
    34081 cggcccggaa gccccagacc ggatcggacc ggaacgagat gagggcgatg gccgggagcc
    34141 cgacatagag cacgccgatc gccgagagcc gcgaggttgc cccgtaggcc acgacgcccg
    34201 cgaggacgcc gcccatggcc acgatcagaa tggcaatggc aggggccttc aggaatgtag
    34261 cgacgatagc cgccaagatc gcgatgccgt ggacgatcat caccgcatcg atctcgccgt
    34321 cgcgtacgac ccggccccac tcccatgtca gcgccatggc aatggcgaga accagtcccg
    34381 cgaaaggcaa cggtccgaga taggtgagga gcagagctgc agccgcaagt gcgagcccgg
    34441 aaatggtgcg ctgcgtaagg tccctgccga agagggagct gagttccggc atcccgagac
    34501 ggctcccttt ttccatcgat gcaggttgtc cgccgatgca gatgcccgat gacgcgtcgc
    34561 ttgcgccgag tggcagaccg agtcgcgcga ccttcaggaa ccgcctagca tgagcttgct
    34621 cggttgtctt ggtcggttgg tggggtgcgg cgacggcggc ggcaggcgcc ggagacatct
    34681 cagccttgcg cattgtcggc attggcccgg atagacggga atgctttatc tggctcgcgc
    34741 atggcggccg agcctcggcg gctggcgccc catcaggaca cgagaagaat gcatcccgcc
    34801 ctcgagttga tcccgagtga tgaggccccg ccacgcgagg tggacgtggt ggtcatcggc
    34861 ggcggcatcg tcggagccgc ggcgacctac gccgttgcca aggcgggcca gcgcgtcgcc
    34921 ctcgtcgaga agggctacgt cggcgccgag cagtcgagcc ggaactgggg ctggtgccgc
    34981 cagcagaacc gcgacgcccg cgagctgccg ctcgccatgc gctccatgga gctctgggag
    35041 agccttggcg gtgagatcgg ccgcgacctg ggctttcgcc gctgcggcct cgtctatgcg
    35101 acgaccgatc ccgcacaggt caagcagtgg gagtcctggc ggtcggtcgc caggagcttt
    35161 ggcgtcgaca cacgcatgct tactggggcc gaggcggcac ggatgtcagg gacgacggcg
    35221 cgcccctggc tcgccggcat ccatt
//
LOCUS       DAORNH010000011        34981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_945_length_34981_cov_2.701225, whole genome shotgun sequence.
ACCESSION   DAORNH010000011 DAORNH010000000
VERSION     DAORNH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_34981_cov_2.701225"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>265)
                     /locus_tag="PK264_02210"
     CDS             complement(<1..>265)
                     /locus_tag="PK264_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HRD74742.1"
                     /translation="EAFPVALAKAGIVFIGPNPKAIAAMGDKIESKKAAAAAKVSTVP
                     GYLGEIADAEAAARIADEIGYPVIIKASAGGGGKGMRIAASRKE"
     assembly_gap    266..289
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<290..523)
                     /locus_tag="PK264_02215"
     CDS             complement(<290..523)
                     /locus_tag="PK264_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRD74743.1"
                     /translation="MFKKILIANRGEIACRVIKTARRMGIKTVAVYSDADRDALHVEM
                     ADEAVHIGASPASVSYLVIDKIVAAAKQTGSQAI"
     gene            complement(675..>1192)
                     /locus_tag="PK264_02220"
     CDS             complement(675..>1192)
                     /locus_tag="PK264_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74744.1"
                     /translation="AGTHPAHVGLLVEAAKEVHSGGGLFHRIGEFPRASDPEFDMHDE
                     ADRVHKNGPPFLQRYLPFWFATFVERMKILLVPLLTMIIPLVKLVPWLYRWRIKRRLL
                     YWYGRLKDLDTSFATDRARVMLAEHRAELDRIDLSVAAIPVPLSFSDQYYALRAAIDS
                     SRQRIFGVVAA"
     assembly_gap    1193..1216
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1217..1996)
                     /locus_tag="PK264_02225"
     CDS             complement(<1217..1996)
                     /locus_tag="PK264_02225"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRD74745.1"
                     /translation="MRQPTPLSRLSGLQLRELVLVVGPALLAVLLAVVAAARFVEPAP
                     PMSLIITTGGEKGGYFAFGRRYAQILAKSGVKLVVEPSAGSIENIKRLGTPEGRWSVA
                     LLQGGIADAKTAPDVLSLGRIFLEPLWVFYRSADSLDRLAQLKGQRIAIGPEGSGTRQ
                     LAVALLSANAITETTATLLPLSGQEAADALDKGAVDAVFLVLAPEAPLIQALLRSTSV
                     KLMSLSQSEAYTRRFPYLSKIVLPRGVVDLVANIPAQDVEMV"
     gene            complement(2124..3188)
                     /locus_tag="PK264_02230"
     CDS             complement(2124..3188)
                     /locus_tag="PK264_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74746.1"
                     /translation="MSFTSVISIAEIDARGSVRETDVARLRRAWRETDEISADDAEVM
                     VALNSRCPVKDRSWSELFIETMTDYIVGQSEPSGYITLKKSQWLVTRLAGSQQSLGRT
                     EHELLVNVLDKARWAPPSLSAFALGQIAIAVSSGIGAYRPDGRAIAGVITELEIDYLR
                     RVLFAYGCGNPSPVTCAEAAVLIDIGERLGPSATHPAWIDLFVRTVANASLAALGYAV
                     PTREEALNPGTAVRADFRRDGAEIVRRVFQSRAQGGAAAGMADPFISSTYRLQSSEER
                     ALARLERQRVEIITQEPMPPADAAWLATRLSGRTELLPGERALIECMSRDCPRLDPAL
                     AAIAARGANDAVGSAAAAMH"
     gene            complement(3304..4635)
                     /locus_tag="PK264_02235"
     CDS             complement(3304..4635)
                     /locus_tag="PK264_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRD74747.1"
                     /translation="MSFPKLFGITDARPADWHDTAPWVRIGNQVTFGLLGGLFLFSAV
                     VTISGAVVASGVVTVENNYKVVQHLDGGIVAKIIAKNGDRVGEGDALVKLDDTQVRAS
                     HQVILIRLFDVVVQQARLEAERDRKSGYAVPDEITARAGETEIARILTTQKNLFEARK
                     TAREGELSVLRQRAGQISEEAKSLEAQLAARLKEQAISMRELANLRPLFEKGFVNQGR
                     IGPVEREAARLEGEVGRLRSEVAKSRGAISEIELKILQIEKDFTQTVVDELRKVQAQV
                     AELSEQRTALEDKLKRTVIRAPRSGRVHALAIHTEGGVITPATAILQIIPDGERLIIE
                     AQVPPTEIDKVRTGLKASIRFPASSFKTTPRLEATVMLVSAAQITDQQGRSYFTVQLE
                     LSAAELKKLPAGHGLIPGMPAEVYIETERRSILSYFVKPLTDALARTFKES"
     gene            complement(4632..>6555)
                     /locus_tag="PK264_02240"
     CDS             complement(4632..>6555)
                     /locus_tag="PK264_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRD74748.1"
                     /translation="AGLAHMPAGTIERLTAFVAGSQETPKPVSVAAPQVKHDAMDTPR
                     LAAGGELWSAVMASRSAFVATGVFSFVINILMLAGPLFMLQVYDRVMTSGSIPTLVAL
                     SIMTAGLYVIIGVLELVRSRIVVRVGVELDRRISDRVFQASLRKSLLSQGASVPALRE
                     LDNLRQFLGGPGPLTFFDAPWTPVYLLVVFLLHWTLGVAATIGAVVLLAIAWASEVRS
                     RAPLMEAGKAAARSLELADTGQRNAEAITSMGMMRAYLERWQKSNREALAWQVLASDR
                     LGSLSSSSKSLRLLLQSMMLAIGAALAIRGDISAGSIVAATIIFGRALAPVEQAIGQW
                     RPFVKAREAYARLDQLLRQCPPEPQRTALPAPKGHLQVSQLRVSAPETRQLILSGLNF
                     EVRPGMVLAVVGPSASGKSTLARAIVGLWPTFGGQITLDGARLDQWSQEALGRHIGYL
                     PQAVELFAGTVKENISRFRANADDAGVIEAARMAHAHDMILALPQGYETQLGNFGTYL
                     SAGQRQRIGLARALYGNPAVVVLDEPNSNLDRVGDEALAAAMDGMRKRGQAGILVSHR
                     VQAIGKADLILYIERGTQRAFGPRNEVLKALQAASGDQAVPQPAPQQKAPAPQPAPAA
                     LSNAKSAAAKPREAKS"
     assembly_gap    6556..6710
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6711..6947
                     /locus_tag="PK264_02245"
     CDS             <6711..6947
                     /locus_tag="PK264_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74749.1"
                     /translation="SGALVSSPAVEWGRVRADGGTSGTAAATRRAAATAAEMGAAADT
                     TAAGAGEATAVGPPSGWATTFALTVGVGTSPAAR"
     assembly_gap    6950..7135
                     /estimated_length=186
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7314..7769
                     /locus_tag="PK264_02250"
     CDS             7314..7769
                     /locus_tag="PK264_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD74750.1"
                     /translation="MTSKKELTRGRCLCRAIEYEFEGKPKWVMHCHCESCRRATSSAV
                     ATYIGVDIGQFRYLKGTPSAYESSPGVNRYFCPTCGSPMAYAGARWPKEVHLFHGTLE
                     NPAQLPPTGHAHVEEQVGWFEVYDDLPRYEQIASKGKAPVRKGPRSGHS"
     gene            complement(7804..8547)
                     /locus_tag="PK264_02255"
     CDS             complement(7804..8547)
                     /locus_tag="PK264_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD74751.1"
                     /translation="MRLTVVGCGDAFGSGGRLQSSYLVDADGERFLIDCGATTLIGLS
                     RLGIDPNSIPTIIISHLHGDHFAGLVWWIMHARHVAKRKVALRIAGPPGIEARVLETS
                     EALYPGSTSIPHGYDLSYQELVAGPPAKVCAVEVSVAEVSHPSGAPSYALRCTLGGRT
                     IAFSGDTEWVEALVGIAAGSDLFLTECHGFERAPPYHLAWKTLVDNLPRLSAQRIMIT
                     HMSEGMLAHRHVAADLGLLVAEEGMVVEI"
     gene            complement(8552..9160)
                     /locus_tag="PK264_02260"
     CDS             complement(8552..9160)
                     /locus_tag="PK264_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD74752.1"
                     /translation="MKLIETKLAPNPRRVRIFLAEKGVAVRTEELDLMKGDLKTPEFT
                     AINPMQRVPVLILDDGTALSETMAICRYFEETVPNPPLMGRDAKDKAIVEMWNRRIEL
                     GLFNHIASAFRHLHPAMAKNEVPQVAAWGEANKPKALEQLAVVDQQLAKSAHIAGDSF
                     SVADITALVAIDFMRLARIQRPPLANLDRWYAAVSARPSAKA"
     gene            complement(9270..10166)
                     /locus_tag="PK264_02265"
     CDS             complement(9270..10166)
                     /locus_tag="PK264_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HRD74753.1"
                     /translation="MQLFFPHVPPLPLPDGHRFPVGKYAMLRQAIESDAGFSRVTLTP
                     ATPASEREIALAHAPDYVAAMTSGRVSPEAMRRIGLPWSDTLIQRSRATVGGAIEAAR
                     QALRTGISGQLAGGTHHAHRDFGSGFCVFNDLAVASLAMLAEGRAGRIAIVDLDVHQG
                     DGNAAILAGDPRVFVLSVHGEKNFPFRKVASTLDVALPDGTADAAYIAALVDALGAIE
                     AFRPDLILYLSGADPLAADRLGRLDLTHAGLIERDRLVMELARRRGIPLSIAIGGGYA
                     DPIEESVRAYHNTFRVALEVFG"
     gene            10412..11413
                     /locus_tag="PK264_02270"
     CDS             10412..11413
                     /locus_tag="PK264_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRD74754.1"
                     /translation="MKVSHGTKKADVLLGALDELNEMWGHTDNDQMFGGRFDDIMRGG
                     KGDDVIWGDRGADLILGNTGDDAIWGSVDNDTILAGVGNDRVSGGKGDDSLAGGKGDD
                     DLHGNTGDDVLAGGSGNDSLAGEAGNDVLDGGAGDDKILAGTGDDLVRASRGADTYVG
                     GTGADTIDFSRIVGKLDVDLGAHAADVAFGANVVRQTLWGFETVIGNDAGGTFKGDKG
                     DTAFLGGAGDDSFRGMGGSDSFLGGSGADLFTILKKDTAGGAVDTIDDFEVGVDHLDL
                     SDFLKGRSGVDAALRLADGGDATTVQGLVGKTWVDVVVLEGVNMADHSADLLGQLLA"
     assembly_gap    11447..11473
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11474..11808)
                     /locus_tag="PK264_02275"
     CDS             complement(<11474..11808)
                     /locus_tag="PK264_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD74755.1"
                     /translation="MAVFRGDDVLIVERGKGRRGVWSLPGGHIEPGEKAQAAAMRELA
                     EETGIEAELVGLVDVHDVLIRREDGALSAHYVLVVFCGHWRSGVPVARSDVVDARFVS
                     LSELPAMALT"
     gene            complement(11862..12614)
                     /locus_tag="PK264_02280"
     CDS             complement(11862..12614)
                     /locus_tag="PK264_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD74756.1"
                     /translation="MAEFSSDGVTIAYLDEGAGEPVLLIHGFASNIAANWVDPGWVRH
                     LTRLGYRVIAMDNRGHGASQKLHATSDYSAPLMAEDARRLLDHLGIERADVIGYSMGA
                     RIGAFLALAHPARVRSLVFGGLGANMIRPMAGTGPIAHALEAASIDDVTNATARTFRA
                     FAESTKSDLKALAACIRSAREPITAEALKTLQCPVLVAVGSEDVIGGSAEDLAALIPN
                     GTFLVIEGRDHMKAVGDRSFKDGVAAFFRERP"
     gene            12707..12955
                     /locus_tag="PK264_02285"
     CDS             12707..12955
                     /locus_tag="PK264_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HRD74757.1"
                     /translation="MAGATIPHFRNDSGVEKIFVGVKEFKCMGASSPYDHPHVFLDMG
                     QDSQALCPYCSTLYLHDSRLHADETEPKGCLADPMEAS"
     gene            12970..14133
                     /locus_tag="PK264_02290"
     CDS             12970..14133
                     /locus_tag="PK264_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD74758.1"
                     /translation="MVGAGIAGLATAIALARRGIASTILERRHLVDEEGAGIQIGPNG
                     CKALAALGLLDQLTDVAGHPGAIHLRDGATGRIQASLPLGAAIRARHGAPYLVAHRAD
                     LHGLLLARTRNLPTVRIETGWDVEVLARHPDGRYQITSSDGRGEIASVLIGADGLWSA
                     VRRFAFPSLATRPRATGLSAMRTVVQTPPDALPHHADINAWMMPGSHVVLYPLRLGSE
                     TAIVVIAPLASEGEGWAFSVDRQSVMTALAHYPPEVRDLAARGTAWRRHQVHRLDALP
                     ALGIERIALVGDAAHPMAPYLAQGAVMALEDALVLADAIARHGPTAMALAEFSRARTR
                     RVRRVVRRSRFNGFVYHQSRPISVARNLVLRNTSGARLLAGLDWLYGWQPDAV"
     gene            14280..15245
                     /locus_tag="PK264_02295"
     CDS             14280..15245
                     /locus_tag="PK264_02295"
                     /inference="COORDINATES: protein motif:HMM:NF025772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4424 family protein"
                     /protein_id="HRD74759.1"
                     /translation="MKLTSPRSRRLHHALAAAAIVVLGENPVTIARDATVEIASSGIA
                     IVASPSLVLRRMDIALDAERIRIDYVVTNLGAEDVNSLITWPQPDIDMTAIGDGALAL
                     PATDPDNVTRHEITSNGARVTPKIERSASAYRLDVTATLREHTLDLFPLADSVLSKLA
                     ALPNPVADHLRELGIVRLDGSRRLPGWTYRSTLFWRQTFKADGDTTISIAFAPVLGSG
                     TLGPDSVDSFSGTHCLPEGRARAIQKKLEAGESVDAVWLTHALTFGSSWSGPVKHARI
                     TIAVPDSETAASTCYGGFKPAGPTLLEWKATDFEPHSDLSILFLR"
     gene            complement(15261..16667)
                     /gene="leuC"
                     /locus_tag="PK264_02300"
     CDS             complement(15261..16667)
                     /gene="leuC"
                     /locus_tag="PK264_02300"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRD74760.1"
                     /translation="MPAPSTMFEKIWSRHVVVDRPDGYTLLFIDRHLLHDGSLPAFER
                     LKRAGLKVRRPDRSFGTPDHYMPTGTRNLADIADARQRHIVSVMRKNADAAGITIFDI
                     GDKRQGIVHVIGPEQGITLPGITLVCGDSHTSTHGALGALAFGIGSSEVTHVMATQTL
                     WQRKPKAMRIEVDGTLGTGVTGKDVILAIIATMGAGGATGHVIEYCGSAIRSLSIEGR
                     LTLCNMSIEGGGRAGLVAPDDKTFAYLAGRPYAPKGADWDQALETWKLLPTDGGAHFD
                     KSLTLNATEIAPMVTWGTSPEDAVAVGGRVPDPAAIDDATRRATVARALAYQGLTPGM
                     AISDIAVDRVFIGSCTNSRIEDLRLAAEVVRGRKAVVPSWVVPGSGLVKEQAEAEGLD
                     RVFEAAGFEWRHAGCSMCVGMNGETGRPQERIASTSNRNFVGRQGRGVRTHLVSPASA
                     AAASVTGRFTDVRRLLAP"
     gene            16830..17486
                     /locus_tag="PK264_02305"
     CDS             16830..17486
                     /locus_tag="PK264_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD74761.1"
                     /translation="MANVRLLIIDDHALFRGALGHSLSATPGRMAISEAGSLDEAMAQ
                     LEAGKEFDLILLDLTMPGVQGLSGLLYLRTEHPGIPVVIVSASDDVAVIRSCMEFGAS
                     GYVPKSQPIEKIREAIESVLAGQVWTPPGLELDGASSPLGDLASSLSTLTPQQVRVLM
                     MLGEGLLNKQIAYKLGVSEATIKAHVSAILQKLGVDSRTQAVIAVNKLNAGALPKPVM
                     "
     gene            17718..18676
                     /locus_tag="PK264_02310"
                     /pseudo
     CDS             17718..18676
                     /locus_tag="PK264_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            18820..19992
                     /locus_tag="PK264_02315"
     CDS             18820..19992
                     /locus_tag="PK264_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD74762.1"
                     /translation="MTSGPRQGGFRPLKVVAKVAESSSVTSFHLEPLTTAAWQAFEPG
                     QFLVLRVPAANGRRAMPRTYSVSWYPGAGGRYRISVKREGAPKPGVPDGAGSSWLHDA
                     VGVGDTIEASGPRGEFVLDRTSDRPVALISGGVGLTPLVAMLHALAGESRRPVRFIHA
                     CENGDVHALRDEVLALSRSRPGIDVHFCYRVPTARDLAAAGHHSVGLLTGGQIDALLA
                     TADMDYYLCGPPPFMKAVYRMLRQRGVAADRIRYEFFGPATVLDEDVAAAPPAIAAES
                     QSSPLLASASVPSPAITVEFRKSGRIVPWTADAKSILDLAEAAGLSPDFSCRAGVCGT
                     CRTRLLAGEVRYFEPPSTNPGQAWRSSAVHVQKARSCLTSRVCKLVRPSYRDGRRN"
     gene            20096..21106
                     /locus_tag="PK264_02320"
     CDS             20096..21106
                     /locus_tag="PK264_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HRD74763.1"
                     /translation="MKVLVTGAAGFIGSATAAALLDRGDEVIGLDNLNAYYDVSLKEA
                     RLGRLTGRAGFSFHRLDLENRAAIADLFARVRPERVVHLAAQAGVRYASENPHAYADA
                     NLTGFLNVLEGCRHTKAGHLVYASSSSVYGANTAMPFSIHQNVDHPLSLYAATKKANE
                     LMAHSYSHLYSLPVTGLRFFTVYGPWGRPDMSLFLFTRKILAGEPIDVYNHGNHARDF
                     TYIDDIVEGIVRTLDRVATPNAGWSSDAPDPGTSAAPYRLYNIGNNNPVELMHFIGCI
                     ETAIGRVAKKNFLPMQPGDVPKTFADVDALIEDVGFKPATPIELGVARFVAWYREFYK
                     VS"
     gene            21147..22430
                     /locus_tag="PK264_02325"
     CDS             21147..22430
                     /locus_tag="PK264_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRD74764.1"
                     /translation="MPLADIGSVKIAVIGLGYVGLPLAAYMARHFPVIGFDIDAERIA
                     ELSAGHDRTREVTADEFAAARALRFSADAAALETANFFIVTVPTPIDTARRPDLTALM
                     RASETVGNAIRDGGVVVFESTVYPGCTEEVCVPIIERLSGLKFNRGFFAGYSPERINP
                     GDKAHRFPDILKVTSGSSAEAAALIDSVYSAVVSAGTHKASSIRVAEAAKVIENIQRD
                     VNIALVNELAMLFDKLGLDTREVLAAAGTKWNFHRYTPGLVGGHCIGVDPYYLTHKAQ
                     SVGFHTEMILAGRRINDGMATWIAHNIIKTMVQHKVRVDSARILIMGFTFKENVPDTR
                     NTKVADLVTELQDFAADVVIYDPHANSREAEHEYGLTIANSLPGGPFEAIVIAVRHDE
                     IVALSAERIRALLAPGGIIYDIKGVLPGSSRTAGL"
     gene            complement(22471..23400)
                     /gene="speE"
                     /locus_tag="PK264_02330"
     CDS             complement(22471..23400)
                     /gene="speE"
                     /locus_tag="PK264_02330"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HRD74765.1"
                     /translation="MDRWIEETFHPHWRVRLKADRILHEVRTEHQHLVIFTNETWGTV
                     LMLDGVTQLTTSDEFVYHEMMAHVPLMAMRSPKDVLVIGGGDGGVLREVLKHPSVERA
                     VLVEIDRTVIDTALEHYPEIAGGAFDDKRTETIIADGLKFVADTDRRFDAIIVDSSEP
                     IGPSAVLHTREFFADCKRALKPGGILVTQNGLPFHHPAHLGRTTEYFQELFANAAPYL
                     CTQPCYFGGPFALNWASDDGSHLKVSRKKLARRAKKRGITTRYWTPAGHRGAFALPGY
                     VAEAAASGDREAAAPAKDKRPRRARKSSSGARA"
     gene            complement(23497..24045)
                     /gene="speD"
                     /locus_tag="PK264_02335"
     CDS             complement(23497..24045)
                     /gene="speD"
                     /locus_tag="PK264_02335"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HRD74766.1"
                     /translation="MAYDDTLFQLGMDLTRSSTAQKEDDAAYACVAHEDRKDFFIERD
                     GVRFAGTHVIIDLFGAKRLDDLKHIERALRRCVEVAGATLLHIHLHHFTPNGGVSGVA
                     VLSESHISVHSWPEADYAAFDVFMCGQSKPELCVDLLKREFQARDVAVKVHRRGEELD
                     EMNWRAASAKKAPVRLKAAAVG"
     gene            24289..25491
                     /gene="nhaA"
                     /locus_tag="PK264_02340"
     CDS             24289..25491
                     /gene="nhaA"
                     /locus_tag="PK264_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HRD74767.1"
                     /translation="MTQQRRASALDFIQHEAAGGIALVVAAIAALLIANSPLASDYKA
                     LQEIPAGVRIGAIGLQKTLLHWVNDGLMAIFFFLVGLEIKREFLVGELSDRQSAALPL
                     FGALGGMIVPAVIYAMMTYRDSSMLSGWAIPCATDIAFAVGVLALLGKRIPPALKLFL
                     LALAIIDDLGAIIIIALFYTADLSAMALVLAAFGIFGLVILNVAGVARLGAYVLLGAF
                     VWICVLKSGVHATLAGVATALALPLGVASREEDGLDGHAGGLQADLHPWVTFAILPLF
                     AFFNAGVSLSGIGVDQVASGITLGIALGLFVGKPIGIVLFTALAIRTGLARLPDGVSW
                     RHMVGCGVLCGIGFTMSLFIGTLAFPDGSREVEVRLGVLLGSLVSAVVGGLMLWRCGR
                     NTEEFEQS"
     gene            complement(25504..27009)
                     /locus_tag="PK264_02345"
     CDS             complement(25504..27009)
                     /locus_tag="PK264_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRD74768.1"
                     /translation="MSASDKSSAEPRDVTHELSLPGPDDRDASADEMSDDRDIAMARR
                     LSRGSIVSLDDEVELEPERPRRWRRALVVGLGLPAALVASLSVLAATTVPTEFIKGRL
                     IAEAEARTGRKVSVNGPTSVSLFPSLSIRLADVTLSSPSDMNVPPLVRVAEIEAETGL
                     ISVLLGRLDVAHLILTRPVMELVVDSRGQRSWDIGVVGGPVRIASVDAGGSTVPGRSL
                     SDTASVVTAPPAPRGGTRPHIGEIRIVDGTLRYMDSRTGVAYEASRIRATATCASERG
                     PLEMRALGVFRGEEVTVKATVHGLAASRTRQPERISVSAASRLGEASFSGTLGDTKVV
                     PGPAASYADGSMLLRSALPDALADWLGVETPSLRAPVSITGRLVVGERSLTVRDAEIK
                     VDKASGKGTITVDLTRERTPVNVDLRVATVGLDALLAAGRVVPPKARPTAASMPASTA
                     PVVTVPTRTAIVPPLNMAKKRADAPRADTPPQTMEELIRRTIGETPPRTSR"
     gene            complement(27119..27736)
                     /locus_tag="PK264_02350"
     CDS             complement(27119..27736)
                     /locus_tag="PK264_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD74769.1"
                     /translation="MKLFWSSRSPFVRKVMVVAHELALVDRIATERVIVSATKPNVEV
                     MKYNPLAKLPTLILDDGRAVYDSRVICEYLDGLAGGTLHPRDPARRLDAIRLNALGDG
                     MMDVLLLWVLERVKPAEQQQQALVEGCRLKLSAVLATLEADAARIEAMPIDIGHLAIG
                     TALGYLDFRFPAEAGWRASHPKLAAWHAKIAARPSFMATMHADVY"
     gene            27905..>28612
                     /locus_tag="PK264_02355"
     CDS             27905..>28612
                     /locus_tag="PK264_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD74770.1"
                     /translation="MTEDGDVAHARPDDPPSAAIDWASIQAGLDTSGFATIRGLLPAR
                     ACSALAGLYDTDTHFRSHINMARHGFGRGEYKYFAYPLPPIIAGLRAMLYAGLAPIAN
                     RWSEQLGSDTRYPETLAAYLKLCHATGQTRPTPLLLRYRSGDHNCLHQDLYGDLVFPL
                     QVATLLAEPGADFSGGEFVLTEQRPRMQSRVRVVPLRRGDAVVFPVHHRPVEGTRGVY
                     RVNMRHGVSEVTAGERHT"
     assembly_gap    28613..28622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            28683..29333
                     /gene="alkB"
                     /locus_tag="PK264_02360"
     CDS             28683..29333
                     /gene="alkB"
                     /locus_tag="PK264_02360"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: protein motif:HMM:NF011930.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HRD74771.1"
                     /translation="MAAGDLFASSATLHRTEIAPGAVLLHGFAADRAELIIAAIDAVA
                     SAAPFRNMITPGGHTMSVAMTSTGRAGWITDRRGYRYSEIDPDTGRAWLAMPAPLHAL
                     AVDAAATGGFPGSEPDSCLVNRYVPGARMSLHQDRDEADLTAPIVSVSLGLPAKFLFG
                     GLRRADRPQRLMLAHGDVVVWGGPSRLAYHGIDPVKDGKHPLTGRCRINLTFRKAL"
     gene            complement(29352..29942)
                     /locus_tag="PK264_02365"
     CDS             complement(29352..29942)
                     /locus_tag="PK264_02365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD74772.1"
                     /translation="MVKMPIIYFVRHGETDWNVAKRYQGQTDTPLNDHGRGQAARNGR
                     ALKELLGSKAAALDYVASPLSRTAETMTIIRREIGLEPDGFRRDDRLKEIHFGHWEGR
                     VWYDLPAEDPEGFAGRKADPWSWRPRQGESYAMLSARVAGWLEGVREPTIAVSHGGVS
                     RVLRGLLLGLTPHDITDLEVPQDKLMVIENGKLAWV"
     gene            complement(29945..>30482)
                     /locus_tag="PK264_02370"
     CDS             complement(29945..>30482)
                     /locus_tag="PK264_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD74773.1"
                     /translation="GAVGSIVGQIAKLKGCRAVGIAGGPEKCRTLVDKLGFDAAVDYK
                     AGNLKRDLEAACPGGIDVYFENVGGEILDTVLPLMNTFGRIPVCGLISAYNATEAVPG
                     PKNLRAILTQRLRVQGLIVFDWNARIPEAVSQLGAWHKEGKLVIREDMRKGGIDAYPD
                     VLNLLYTGGNDGKLVLEV"
     assembly_gap    30483..30525
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30526..30943)
                     /locus_tag="PK264_02375"
     CDS             complement(<30526..30943)
                     /locus_tag="PK264_02375"
                     /inference="COORDINATES: protein motif:HMM:NF028194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD74774.1"
                     /translation="MAEQNRMVKLARRPRGMAQRSDFTIEDAPISEPGPGEFRVKVGC
                     ISLDPAMRGWMNDSKSYVKPVGIGEVMRAYAAGIVEASNNPSFKSGDAVQGLFGVQRY
                     AISKGERVVKIDLGAAPMARWVGGLGMPGWTAYFGLL"
     gene            31265..32170
                     /gene="cysD"
                     /locus_tag="PK264_02380"
     CDS             31265..32170
                     /gene="cysD"
                     /locus_tag="PK264_02380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRD74775.1"
                     /translation="MSHLEQNLATLEAEAIHVFREAAAQFRRPVMLYSIGKDSTVLLH
                     LARKAFHPAPLPFPLLHIDTGWKFREMIGFRDRIAQSLGLDLIVHVNREGETAGVSPV
                     THAPSVYTDVMKTRPLKEALDRYAFDAAFGGARRDEEASRAKERIFSVRSAGHRWDPR
                     KQRPEMFGLLNGRLGSGETMRVFPLSNWTEGDVWRYVLREGLEIVPLYLAADRPTITR
                     AGQILVLDDERLPLLPGEAVETRRVRFRTLGCWPLTAAVASSADTLEAVVRETLAARS
                     SERQGRLIDKDQSSAMERKKLEGYF"
     gene            32239..>33532
                     /locus_tag="PK264_02385"
     CDS             32239..>33532
                     /locus_tag="PK264_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRD74776.1"
                     /translation="MVRVLTCGSVDDGKSTLIGRLLWDAADLPDDTRATIMASAGADG
                     IPDVSLLLDGLEAERAQGITIDVAWRYFDLASRRYVIIDCPGHEQYTRNMATGASQAD
                     IAIMLIDARQGIKLQTRRHAAILELVGIKEVILAINKMDLVDWSEAVYRAIADEFAAL
                     SARSGFERAVAIPVASKPGDNIARRSERLRWFNGPTLLDALEATSRSLATATRPFRFP
                     VQRVTRASRDFRGLAGTVASGGVAVGSEIVEVLSGKRALVKRIATFDGDQPTATAGDA
                     VTIVLDRDLDIARGGLLASPSEPATAATDLEVQLVWLADTGPGHSGLLFRTTTDLVPV
                     SEFRVIARMDLQTLQKTAAASCGANDVADARIKLGRGVAVDRFAADHATGHFILVDAL
                     TGATVAGGIVADIVQDKEARSGDVFRLTRALLETTVCAD"
     assembly_gap    33533..33662
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(33732..34769)
                     /locus_tag="PK264_02390"
     CDS             complement(33732..34769)
                     /locus_tag="PK264_02390"
                     /inference="COORDINATES: protein motif:HMM:NF036814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HRD74777.1"
                     /translation="MTWPKPHIPTRVNVLGNDTDIDGDTLRVIAAHSPGGTVEINPDG
                     TLTFTANGGFAGETTITYVISDGQGGSSIATVRVNVLDVVPPAADSARPADTPRAAQA
                     AEPLAADGMVIDAVQRIGGPGRLIGDFGSHGIVVAAVNQVASLDGVSGSGYIGVVDGG
                     SPINSNRIWEIQRSLGSAGLGFHRGDNTGMPEGLTGFSLRTSLLGHTIGDGSQTELII
                     QTLVRERMLMIQISNMAMDQAKTVSEYRVMLADGQPLPAWLDRPRGDLLIGERPVDSV
                     SLRLKITVLYSDGSFETKLVEIQTGSGEIHPIEAKRGVDLPVPFVNQFALKTPPSDDG
                     LDALARYLKAS"
BASE COUNT         5796 a        11642 c        11145 g         5799 t
ORIGIN      
        1 cctccttgcg gctcgccgcg atgcgcatac ccttaccgcc gccgccggcc gaagccttga
       61 tgatcaccgg gtagccgatc tcgtcggcga tgcgggccgc cgcctcggca tcggcgatct
      121 cgcccaaata gccggggacg gtcgagacct tcgctgccgc cgcggccttc ttgctctcga
      181 tcttgtcgcc catggccgcg atcgccttgg gattgggccc gatgaacacg atgccggcct
      241 tagcgagtgc caccgggaac gcctcnnnnn nnnnnnnnnn nnnnnnnnng atggcttgcg
      301 agccggtttg cttggcggcg gcgacgatct tgtcgatgac gaggtaggag accgaggcgg
      361 gcgaggcacc gatgtgcaca gcctcgtccg ccatctcgac gtgcagcgcg tcgcggtcgg
      421 cgtccgagta gaccgccacg gtcttgatgc ccatgcgccg cgccgtcttg atgacgcggc
      481 aggcgatctc gccacggttg gcgatcagga tcttcttgaa cattattgtg tgccgatgtc
      541 gttcgggtgg gccgttatgc acgtcggatg tcgaccgctg ttctactgaa ccgcggcgga
      601 ggggtaaaca tggtctatcg attgagatgg ttagcccggg aggcatgccg attcgcgcgt
      661 ctcgcggcgg ctcatcacgc cgccacgaca ccgaaaatgc gctgccgcga gctgtcgatc
      721 gcagccctca gcgcgtagta ctggtccgag aagctcagcg gcacgggaat ggctgcaacc
      781 gacaggtcga tcctgtcgag ctccgcccgg tgctcggcga gcatcacccg cgcgcggtcg
      841 gtcgcaaagc tcgtatcgag gtccttgaga cggccgtacc agtagaggag ccgccgcttg
      901 atgcgccagc ggtagagcca cggcaccagc ttcacgagcg ggatgatcat ggtcagcagc
      961 ggcacaagca gtatcttcat ccgctcgacg aatgtcgcga accagaacgg aaggtagcgc
     1021 tgcaggaaag gcgggccgtt cttgtggacg cggtccgcct catcgtgcat gtcgaactcg
     1081 gggtcgctcg cccgggggaa ctcgccgatc cgatggaaca gacctccgcc cgagtgcacc
     1141 tccttggccg cctcgacgag caaccccaca tgggcggggt gcgtgcccgc ctnnnnnnnn
     1201 nnnnnnnnnn nnnnnngacc atctccacgt cctgcgccgg aatgttcgcg accagatcga
     1261 ccacgccgcg cggcaatacg atcttcgaga gatagggaaa gcgccgtgta tacgcctcgg
     1321 actggctcaa gctcatgagc ttgaccgacg tgctgcgcag cagcgcctgg atgagcggag
     1381 cctccggagc aagcaccaga aaaaccgcgt caaccgcacc cttgtcgagc gcgtccgctg
     1441 cctcctgccc gctcagcggc agcagcgtcg cggtcgtctc cgtgatggcg ttggctgaca
     1501 gcagcgcgac ggctagctgc cgcgtgccgc tgccttcagg gccaatggcg atgcgctggc
     1561 ctttgagctg cgcgaggcga tcgaggctgt cggcgctgcg atagaatacc cacagcggct
     1621 cgaggaagat gcggccgagc gagaggacgt cgggcgccgt cttggcatcg gcgatgccgc
     1681 cctggaggag ggcaacgctc cagcggcctt cgggcgtccc gagccgcttg atattctcga
     1741 tcgagcccgc cgacggctcg acaaccagct tcacccccga tttcgcgagg atctgggcat
     1801 agcgccttcc aaaggcgaag tagccgccct tctcgccgcc agtcgttatg atgaggctca
     1861 tgggcggcgc cggctcgacg aagcgggcag cggcgacgac cgcgagcagg acggcaagta
     1921 gcgcaggtcc gacgacgaga acgagttccc ggagctgaag tccggaaagt cgggacaacg
     1981 gtgtcggctg tcgcatcaat caccttcgag ctgcatcgcc gggcggattc tcccaggcat
     2041 cggaagatat ccgccttgct gatacaggca gtgatcggtc gatctcaata ccaagctcgc
     2101 cctcgcgaca aatcgctcag gcctcaatgc atcgcggctg cggccgagcc gacggcgtcg
     2161 ttcgcgccac gagccgcaat ggccgccagg gccggatcga ggcgcggaca atcgcgggac
     2221 atgcattcaa tcagcgcccg ctcgcctggc agcagctccg ttcgacccga gaggcgggtt
     2281 gcgagccagg ctgcgtccgc aggtggcatc ggctcctggg tgatgatctc gacgcgctgg
     2341 cgctcgaggc gtgcgagcgc gcgctcctcg gaggattgca ggcgataggt gctggagatg
     2401 aaggggtccg ccatgccggc agccgcgccg ccctgcgccc tgctctggaa gacccgacgc
     2461 acgatctcgg caccgtcacg cctgaaatcg gcccgcacgg ccgtgccggg gttcaacgcc
     2521 tcctcacggg tcggcaccgc gtagccgagg gcggcgagcg aggcgttggc gacggtgcgc
     2581 acgaagagat cgatccaggc cggatgcgtc gcggaagggc ctaggcgttc gccgatatcg
     2641 atgaggaccg ccgcctcggc gcaggtgacc ggcgagggat tgccgcagcc gtaggcgaac
     2701 agcacgcgcc tgagatagtc gatctcgagc tcggtgatga cgcctgcgat cgcccggccg
     2761 tcgggtcggt aagcgccaat cccggaggaa acggcgatcg cgatctggcc gagagcgaag
     2821 gccgaaaggc tgggcggcgc ccagcgggcc ttgtcgagaa cgttgacgag cagctcgtgc
     2881 tcggtgcgcc cgaggctctg ctggctgccc gcgaggcgcg tgaccagcca ctgcgatttc
     2941 ttgagcgtga tgtagccgct cggctcactc tggccgacga tgtagtcggt catggtctcg
     3001 atgaagagct cgctccagga gcgatccttc accgggcatc gggaattgag cgcgaccatc
     3061 acctcggcgt catcagccga gatttcgtcg gtctcccgcc atgcacggcg gagcctcgcg
     3121 acgtcggtct cgcggacgct gccgcgcgcg tcgatctctg caatggaaat aacgctcgta
     3181 aagctcatgg aacatccacc cggccacagg tcccgacccg cttcgaccgt aggggagggt
     3241 ggttagcaac tcgctaacgg ccgctccgat gtgagcctgc gtgtcccaat cgacacaacc
     3301 ggctcagctc tccttgaagg tgcgtgcgag cgcgtccgtc aacggcttca cgaaatagga
     3361 gaggatcgag cgccgctcgg tctcaatgta gacctcggcc ggcatgccgg ggatgagtcc
     3421 gtggccggcc ggcagtttct tgagctctgc cgccgaaagt tcaagttgca cggtgaagta
     3481 cgagcggccc tgctggtccg tgatctgcgc cgccgatacg agcatgaccg tggcctcgag
     3541 ccggggcgtc gtcttgaagc tcgacgccgg gaagcggatc gatgccttga ggcccgttcg
     3601 caccttgtcg atctccgtcg gcggcacctg cgcctcgatg atgagccgct ccccgtccgg
     3661 gatgatctgc aggatagccg tcgcaggcgt gattacgccg ccctcggtat ggatggcgag
     3721 cgcatggacg cggcccgagc gcggggcgcg gatcaccgtg cgtttcagct tgtcctcgag
     3781 cgccgtgcgc tgctcgctga gctcggccac ctgagcctgc accttgcgca gctcgtcgac
     3841 gaccgtctgc gtgaagtcct tctcgatctg gaggatcttc agctcgatct ccgaaatggc
     3901 gccgcgcgac tttgcgacct cgcttcggag gcggccgacc tcgccctcga gccgcgccgc
     3961 ctcgcgctcg accggcccga tgcggccctg gttgacgaag cccttctcga acagcgggcg
     4021 gagattggcg agctcgcgca tcgagatcgc ctgctccttg aggcgggccg caagctgtgc
     4081 ctcgagactc ttcgcctcct cgctgatctg gccggcgcgc tggcggagca cggagagctc
     4141 gccctcccgc gccgtcttgc gggcctcgaa gaggttcttc tgcgtcgtca ggatgcgcgc
     4201 gatctcggtc tcgccggccc gcgccgtgat ctcgtcgggt acagcgtatc cgctcttgcg
     4261 atcgcgctcg gcctcgagcc gcgcctgctg caccacgaca tcgaagagcc ggatcaggat
     4321 cacctgatgg gaggcgcgca cctgcgtatc gtcgagcttc acgagcgcgt caccctcgcc
     4381 gaccctgtcg ccgttcttgg cgatgatctt ggcgacgatg ccgccgtcga ggtgttggac
     4441 gaccttgtag ttgttctcga ccgtgacgac gccgctcgcg acgacggcac cgctgatggt
     4501 gacaactgcc gagaagagga agagcccgcc gaggagccca aacgtcacct ggttgccgat
     4561 ccgcacccag ggcgctgtgt cgtgccaatc agccgggcga gcatccgtga tgccgaaaag
     4621 cttggggaag ctcatgactt ggcctctctc ggcttcgcgg cggccgactt ggcattggac
     4681 aaggctgccg gtgccggctg aggagccggc gccttctgct ggggagccgg ctgcggcacg
     4741 gcctgatcgc cggacgccgc ctgcagtgcc ttcagcacct cgttccgcgg tccgaaggcg
     4801 cgctgcgtgc cacgctcgat gtagagaatg aggtctgcct tgccgatcgc ctgcacgcga
     4861 tgggagacga ggatgccggc ctggccgcgc ttgcgcatgc cgtccatcgc cgccgccagc
     4921 gcctcatcgc cgacccggtc gaggttcgaa ttgggctcgt cgagcacgac gaccgccgga
     4981 ttgccataga gcgcgcgagc aaggccgatg cgctggcgct ggcctgcgga gagataggtg
     5041 ccgaagttgc cgagctgggt ctcatagccc tgcggtaagg cgaggatcat gtcgtgcgcg
     5101 tgcgccatgc gggccgcctc gatgacgcct gcgtcgtcag cgttggcgcg aaagcgcgaa
     5161 atgttctcct tcacggtgcc ggcgaaaagc tccaccgcct gcggcagata gccgatgtgg
     5221 cggccgaggg cctcctgcga ccactggtcg aggcgcgcgc cgtcgagcgt gatctgtccg
     5281 ccgaatgtcg gccagagccc gacgatcgcc cgcgccagcg tcgacttccc cgaggccgag
     5341 ggcccaacga cggcgagcac catgccgggc cgcacctcaa aattgagacc ggagagaatg
     5401 agctggcgcg tctccggggc ggagacgcgg agctggctca cctggaggtg ccctttcggc
     5461 gcggggagtg cggtccgctg tggctccggt gggcattggc ggagcagctg gtcgaggcgg
     5521 gcataggcct cgcgcgcctt gacgaatggc cgccactgac cgattgcctg ctcgacgggc
     5581 gcaagcgcac ggccgaaaat gatcgtcgcc gcgacgatcg aacctgcgct gatgtcgcct
     5641 ctgatcgcga gtgccgctcc aatggcgagc atcatcgact gcaggagcag gcggagtgac
     5701 ttcgacgagg aggataggct gccgagccgg tcggaggcga gcacctgcca ggcgagcgcc
     5761 tcgcggttag atttctgcca gcgctcgaga taggcgcgca tcatgcccat ggaggtgatc
     5821 gcctcggcgt tgcgctgccc cgtatcggca agctcgagac tgcgtgctgc ggccttgccc
     5881 gcttccatca ggggcgcccg gctccgcacc tcgctcgccc aggcgatcgc cagaagcacc
     5941 acagcgccga tggtggcggc tacgccaagc gtccagtgca agaggaagac gacgaggaga
     6001 tagaccggcg tccagggcgc atcgaagaag gtgagaggcc ccgggccgcc gaggaactgg
     6061 cggaggttgt cgagttcgcg aagcgccggt accgatgcgc cctgggatag caggctcttc
     6121 cggagcgagg cctggaacac gcggtcggat atgcgccggt cgagctcgac accgacgcgc
     6181 acgacgatgc gcgagcgcac cagctcgagg acgccgatga tcacgtagag acccgccgtc
     6241 atgatcgaaa gcgcgaccag tgtcggtatg ctgcccgaag tcatgacgcg gtcgtagacc
     6301 tgcagcatga agagcgggcc ggccagcatc agaatattga tcacgaacga gaacactccc
     6361 gtcgcgacaa atgcgctgcg gctcgccatg accgccgacc agagctctcc gccggccgcc
     6421 agcctcggcg tgtccatggc gtcgtgcttg acttgcggcg cggcgacgct gaccggcttc
     6481 ggtgtctcct ggctcccggc aacgaacgcg gtgaggcgct cgatcgtgcc agccggcatg
     6541 tgggcgagcc ctgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcggagcgc
     6721 tcgtgtcgtc gccggcagtg gaatggggcc gcgtccgcgc cgacggcgga acctcgggaa
     6781 cggcggccgc gaccagacga gcggcggcga cggccgccga gatgggcgcc gctgcagaca
     6841 ccaccgcggc cggtgccggc gaagccacag ctgtcggtcc gccgagcgga tgggcgacca
     6901 ccttcgcctt gacggtggga gtagggacct ctccagcggc gcgctgagcn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgagc
     7141 aatcccttaa gggtgagcga tgtgttaaac tcgcgctaac aagtccacgc gtttcccatg
     7201 ggccaacatt ggcgcctcgg cccgaccggc ctgcccatag acgagcagac ggccaccatc
     7261 taacctccgg gtacatcgcc gaagtgcgag ccccgattgt gaggagtgtg cccatgacct
     7321 cgaagaagga attgacccgc ggccgctgcc tctgccgcgc catcgagtac gagttcgagg
     7381 ggaagcccaa gtgggtcatg cactgccact gcgaaagctg ccggcgcgcg acctcgtctg
     7441 ccgttgcgac ctacatcggc gtcgacatcg gccagttccg ctacctgaag ggcacgcctt
     7501 cggcctatga atcctcgccg ggcgtgaacc gctacttctg cccgacctgc ggctcgccca
     7561 tggcctacgc cggcgcacgc tggcccaaag aggtgcatct cttccacggc acgcttgaga
     7621 acccggcgca gttgccgccg accggacacg ctcacgtcga ggagcaagtc ggctggttcg
     7681 aggtgtatga cgacctgccg cgctacgagc agatcgcctc gaagggcaag gcgcccgtaa
     7741 ggaagggccc gcggtccggg cattcgtgag aggcctgccg tcaacgacgc gcggcaacgg
     7801 cgatcagatc tcgacgacca taccttcctc ggcaaccaga agcccaaggt cggctgcgac
     7861 atgacgatgg gcgagcatgc cctcgctcat gtgcgtgatc atgatgcgct gcgctgagag
     7921 gcgcggcaag ttgtcgacga gcgtcttcca ggcgagatga tagggcggcg cacgctcgaa
     7981 accgtggcac tcggtcagga aaaggtccga gccggccgcg atcccaacca gcgcctcgac
     8041 ccattccgta tcgccggaaa acgcgatcgt cctgccgcca agcgtgcagc gcagcgcata
     8101 agacggtgcg cctgagggat gcgaaacctc agcgacagac acttcgacgg cgcagacctt
     8161 ggccggcggc cccgccacca gttcctggta gctgagatcg tagccgtgtg ggatcgacgt
     8221 cgagcccgga taaagcgcct cgctcgtctc cagcacgcgt gcctcgatgc ccggtgggcc
     8281 ggcgatccgc agcgccacct tccgcttggc gacgtggcgg gcatgcatga tccaccacac
     8341 caaacctgcg aaatgatcgc cgtgcaggtg cgagatgatg atcgtcggga tgctgttggg
     8401 gtcgatgccg aggcgcgaca ggccgatcag tgtagtcgcg ccgcaatcga tgagaaagcg
     8461 ctcgccatcc gcatcaacga ggtagctgct ctgcaaccga ccgccggagc cgaaagcgtc
     8521 gccgcaaccg acgaccgtga ggcgcatgat ctcacgcctt ggcgcttgga cgcgccgaga
     8581 cggcagcata ccagcgatcg aggtttgcga gcggcggacg ctgtatccgc gcgagccgca
     8641 tgaagtcgat ggcgacaagc gctgtgatat cggccaccga gaaactgtcg ccggcaatat
     8701 gggcagattt cgcgagttgc tgatcgacga ccgccagctg ctccagcgcc ttcggtttgt
     8761 tggcctcgcc ccacgccgcg acctgcggca cctcgttctt ggccattgct ggatggagat
     8821 ggcggaaggc cgaggctatg tgattgaaga gcccgagctc gatccgccgg ttccacatct
     8881 cgacgatagc cttgtccttg gcatcgcggc ccatcagcgg cgggtttggc accgtctcct
     8941 cgaaatagcg gcagatggcc atggtttcgg agagcgccgt gccgtcgtcg agaatgagaa
     9001 cgggcacgcg ctgcatcggg ttgatcgctg tgaactcggg cgtcttgaga tcacccttca
     9061 tgaggtcgag ttcctcggtc cgcaccgcga cgcccttctc ggcgaggaaa atacggaccc
     9121 ggcggggatt tggcgcgagc ttcgtctcga tgagcttcat ggcgactttt tcccgagtgg
     9181 cgatttcgtc atccgacaat tggtactgga gacgtgtggc tcgagcaagg agacggcgcc
     9241 gccgcccaac ccggattgct tctgtgtggc tagccgaaca cctcgagcgc gacacgaaat
     9301 gtattgtggt aggcgcgcac gctctcctcg atcggatcgg catagccccc gccgatggcg
     9361 atcgagagcg gaatgccgcg tcgccgtgca agctccatca cgaggcgatc gcgctcgatg
     9421 aggcccgcgt gcgtgagatc gaggcgcccg agccggtcgg ccgcgagcgg atcggcaccg
     9481 gagagataga ggatcagatc aggcctaaac gcctcgatcg cgccgagcgc atcgaccagc
     9541 gcggcgatat aggccgcatc tgccgttcca tcaggaagcg cgacatcgag tgtggacgcc
     9601 accttgcgga atggaaaatt cttctcgccg tggacgctca gcacaaagac gcgcggatcg
     9661 cctgcaagaa ttgcggcatt tccgtcaccc tggtgcacgt cgaggtcgac gatggcgatg
     9721 cggccggcgc gtccctcggc gagcatcgca aggctcgcta cggcgagatc gttgaacacg
     9781 cagaagcccg agccgaaatc gcggtgcgca tggtgcgtac cccccgcgag ctgaccggat
     9841 atgcctgttc tcagcgcctg ccgcgcagcc tcgatcgcgc cgccgacggt cgcacgtgac
     9901 cgctggatga gcgtatcgct ccacggcaat ccgatgcgcc gcatggcctc tggcgagacc
     9961 cgccccgacg tcattgcggc gacatagtct ggcgcatgcg ccagcgcaat ctctcgctcg
    10021 gatgcgggcg tcgcaggtgt cagcgtgacg cgcgagaagc ccgcgtcgct ctcgatcgcc
    10081 tggcgcagca tcgcatactt gccgacggga aagcggtgcc catccggcag cggcagcggc
    10141 ggtacgtgag gaaagaagag ctgcatcgcg gggcaccgat cggctgagct tcgcggaaac
    10201 gtgaccgggg aagttctcgt caaattagat gcaatttgag cgacccgcat gaggcttttc
    10261 tcagcatctc tcagccatct tctaacggcg agtgaacgga gactccgaca ttcgagtgaa
    10321 gtcgttcgct gatttgacgg gaaaatcccg agactgtgtg gccgcagatt aaccattgcg
    10381 ggccgggagc gcgcaactga ggagctgacg catgaaagta tcgcacggaa ccaagaaggc
    10441 cgacgtcctg ctcggcgcgc tcgatgagct caacgaaatg tgggggcaca ccgacaacga
    10501 tcagatgttc ggcggacgct tcgacgacat catgcgtggc ggcaagggcg acgacgtcat
    10561 ctggggcgat cgcggcgccg acctcatcct cggcaacacc ggtgacgacg ccatctgggg
    10621 cagcgtcgat aacgacacga tcctggccgg cgtcggcaac gaccgcgtct ccggcggcaa
    10681 gggcgacgac agcctcgcag gcggcaaggg cgacgacgat ctccacggca acacaggcga
    10741 cgacgtcctc gccggcggca gcggcaacga cagcctcgcg ggcgaagctg gcaacgacgt
    10801 cctcgacggc ggcgccggcg acgacaagat cctcgctgga acgggcgacg acctcgtccg
    10861 cgcgagtcga ggcgccgaca catacgtcgg cggcaccggc gccgatacga tcgacttctc
    10921 gcgcatcgtc ggaaagctcg acgtcgacct cggcgcgcac gcggccgatg tcgcgttcgg
    10981 tgccaatgtc gtgcgccaga cgctctgggg cttcgagacg gtaatcggca acgatgccgg
    11041 cggcaccttc aagggcgaca agggcgacac ggcgttcctc ggcggcgcgg gcgacgacag
    11101 cttccgcggc atgggcggct ccgacagctt cctcggcggc tcgggcgcgg atctcttcac
    11161 gatcctcaaa aaggatacgg ccggcggcgc ggtcgatacg atcgacgact tcgaggtcgg
    11221 cgtcgaccac ctcgacctct ccgacttcct caaaggtcga tcgggtgtcg acgccgccct
    11281 ccggctcgcc gacggcggcg acgcgaccac ggtgcaaggc ctcgtaggca agacctgggt
    11341 ggacgtggtg gtgctcgagg gcgtcaacat ggccgaccac agcgccgacc tgctcggcca
    11401 gctgctcgcc taggccgacg tcgccttgct ggcaacgaca cggcggnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnngtcagcg ccatcgccgg caactcgctc agcgagacga agcgcgcgtc
    11521 gacgacatcg gaccgggcga ccgggactcc ggatcgccag tggccgcaga agacaacgag
    11581 gacgtagtgc gccgacaagg cgccgtcctc gcgccggatc agcacatcgt gcacatcgac
    11641 gagacccacg agttcagcct cgatcccggt ctcctccgca agctcgcgca tggctgccgc
    11701 ctgcgccttc tcaccgggct cgatgtgtcc accgggcagg ctccacacgc cccgccggcc
    11761 cttgcctcgc tcgacgatca gcacgtcgtc gccgcggaat acggccatgc tggccgccgg
    11821 gcggggccat tccggccgct gcaagggttc gtgcgccatc gtcatggacg ctcacggaaa
    11881 aatgcagcga cgccatcttt gaagctccgg tcgccgaccg ccttcatgtg atcgcgcccc
    11941 tcgatgacga gaaacgtccc atttggaatg agggccgcga ggtcctccgc cgagccgccg
    12001 atcacatcct ccgagccgac cgcgaccagc accgggcact gcagcgtctt cagggcctcg
    12061 gcggtgatcg gctcgcgggc cgaacggatg caggcggcga gcgccttcag gtcgctcttc
    12121 gtcgattccg cgaatgcccg gaaggtgcgc gccgtggcat tggtcacgtc gtcgatgctc
    12181 gccgcctcga gcgcatgggc gatggggcct gtgcccgcca tcggccggat catgttggcg
    12241 ccgaggccgc cgaagacgag gctgcgcacg cgggcgggat gggcaagcgc caggaacgcg
    12301 ccgatgcgtg cgcccatcga atagccgatc acgtcggcgc gctcgatccc gagatgatcg
    12361 aggaggcgac gcgcatcctc cgccatcagc ggcgcgctgt agtccgaggt tgcatgcagc
    12421 ttctggctcg cgccgtggcc gcgattgtcc atggcgatga cgcggtagcc caagcgcgtc
    12481 agatggcgca cccagcccgg atcgacccag ttcgcggcga tgttcgacgc gaagccatga
    12541 atgaggagga cgggttcgcc ggcgccttca tcgaggtagg cgatcgtcac accgtcggac
    12601 gagaattccg ccaccgctgc ctccgttcca aatgcgacgg ctcaccctct atcatttggc
    12661 gctccacctc gctatggtcc cgccgagcaa tccggaggag ccgaccgtgg ccggcgcgac
    12721 tatcccgcat ttccgcaacg acagcggcgt cgagaagatc ttcgtcggcg taaaggaatt
    12781 caagtgcatg ggcgcgagtt cgccctacga ccatccgcac gtcttcctcg acatgggcca
    12841 ggacagccag gcgctctgcc cttactgctc gacactctat ctccacgact cgcgcctgca
    12901 tgccgacgag acggagccca agggctgcct cgccgatccg atggaggcga gctgagcgag
    12961 ccgatcgtca tcgtcggcgc cgggatcgcg ggcctcgcca cggcgatcgc gctcgcccgc
    13021 cgcggaatag cctcgacaat cctcgagcgt cggcacctcg tcgatgagga aggcgccggc
    13081 atccagatcg ggccgaacgg ctgcaaggcg ctcgccgcac tcggtctcct cgaccagctg
    13141 acagacgtcg ccggccatcc gggggccatc catttgcgcg acggcgcaac cggccggatc
    13201 caggcatcgc tgccgctcgg ggcagcgatc agagcgcggc acggcgcccc ttatctcgtc
    13261 gcccatcgcg ccgacttgca cggcctgctg cttgcgcgga cgcggaacct acccacggtg
    13321 cggatcgaaa caggctggga cgtcgaggtt ctcgctcgcc atccggatgg ccggtaccag
    13381 atcacttcaa gcgacggtcg gggcgagatc gcgtcggtac tgatcggagc ggacggcttg
    13441 tggtcggcgg tgaggcgctt cgcgttcccg agccttgcga cgcggccgcg cgcaaccggc
    13501 ctcagcgcga tgcgcaccgt ggtccagacg ccacccgatg ccctgccaca ccacgcagat
    13561 atcaatgcct ggatgatgcc gggctctcac gtggtcctct acccgctgcg cctcggcagc
    13621 gagacggcca tcgtggtcat agcgccactc gccagtgaag gtgagggctg ggccttctcg
    13681 gtggatcgcc agtccgtcat gacagcactc gctcactatc cgcccgaggt tcgcgacctg
    13741 gccgctcgcg ggacggcgtg gcgacgacac caggtccatc ggctcgacgc gctgccggca
    13801 ctggggatcg aacgcatcgc cctcgtcggc gatgccgctc acccgatggc cccctatctt
    13861 gcgcaaggcg ccgtgatggc actggaggat gccctcgttc tggcggacgc gattgcgcgt
    13921 catgggccaa cggccatggc gctcgcggaa ttcagccggg cccgtacccg gcgcgtgcgg
    13981 cgcgtcgtac gccggtcacg cttcaacggc ttcgtttacc atcagtcgcg gccgatcagc
    14041 gtagcccgca acctcgttct gcgaaataca tccggcgcgc gccttctggc gggcctcgat
    14101 tggctttacg gatggcaacc cgacgccgtc tgagcgtgcg aggcggcaac acttgctccg
    14161 aattcgccat acagcctcga cgcgcatcat cccctgcccc tacaattccc gatatctttg
    14221 gctcgcggac gacctcggcc gcagcggccg gtagtgttgc tcgttcaagt gagatgcgta
    14281 tgaaactcac atctccgcgc agtcgtcggc tgcaccacgc gctggcggca gcggcgatcg
    14341 tcgtccttgg cgagaaccct gtgacgatcg cacgggacgc gaccgtcgag atcgcgagct
    14401 ccgggatcgc catcgtcgca agtccttcgc tggtgctgcg ccgcatggac attgcactcg
    14461 atgccgagcg gatcaggatc gactacgtcg taaccaacct cggcgccgag gacgtgaact
    14521 cactgatcac ctggccgcaa cccgacatcg acatgaccgc catcggcgat ggcgcgctgg
    14581 ctttgccggc gacggatccc gacaatgtca cgcgtcatga gatcaccagc aacggcgctc
    14641 gcgtcacgcc gaagatcgag cgttcggcga gcgcctaccg cctggatgtg actgcaacgc
    14701 tgcgggagca caccctggat ctgtttcctc tggccgacag cgtgttgtcg aagctggccg
    14761 cacttccaaa tcccgtcgcc gatcatctgc gcgaactcgg catcgtccgc ctcgatggct
    14821 cgcgccggct cccgggatgg acctaccgct cgacgctctt ctggcggcag acattcaagg
    14881 cggacggcga caccacgatc tccatcgcct tcgcgcctgt gctggggtca ggtacgctcg
    14941 ggccggattc tgtcgattcg ttcagcggca cccactgcct tcccgaaggg cgcgcgcgcg
    15001 cgatccagaa gaagctcgaa gccggcgagt ctgtcgatgc cgtatggctg acccatgcgc
    15061 tgacttttgg ctcgagctgg tccggaccag tgaagcacgc gcgcatcacg atcgcggtgc
    15121 cggacagtga gacggcggcc tcgacctgct acggcggctt caagccggcg gggcccacac
    15181 tgctcgaatg gaaggccacg gatttcgagc cgcacagcga cctctcgatc ctgttcttgc
    15241 gctgacatgc ctgcccatga tcacggcgcc agcaggcgcc gcacgtccgt gaagcggcct
    15301 gtcaccgatg ccgctgcagc gctcgccggc gagacgagat gcgtgcgcac gccgcgtcct
    15361 tgacgaccga cgaagttgcg gttcgacgtc gaggcaatgc gctcctgcgg ccggccggtc
    15421 tccccgttca tgccgacgca catcgagcag ccggcgtgcc gccactcgaa gccggcagcc
    15481 tcgaagaccc tatcgaggcc ctcggcctcg gcctgctcct tgacgaggcc cgagcccggc
    15541 acgacccagc tcggcacgac ggccttgcga ccccgcacga cctcggcggc aagcctcaga
    15601 tcctcgatcc ggctgttcgt gcaagagccg atgaaaaccc gatcgacggc aatgtcgctg
    15661 atcgccattc ccggcgtcag gccctggtac gcgagcgcgc gcgcgaccgt cgcccgccgc
    15721 gtcgcatcgt cgatggccgc cggatcgggc acccgccccc cgacggcaac ggcgtcttcc
    15781 gggctcgtgc cccaagtgac catcggcgcg atctcggtag cattcaaggt aagtgacttg
    15841 tcgaaatggg cgccgccgtc ggttggcagg agcttccagg tttccagcgc ctggtcccag
    15901 tcggccccct tcggcgcata gggccggccg gccagatagg cgaacgtctt gtcgtcgggc
    15961 gcgacgaggc ccgcccgtcc gccgccttcg atggacatgt tgcagagcgt caaccggccc
    16021 tcgatggaaa ggctccggat tgccgagccg caatattcga tgacgtgtcc ggttgccccg
    16081 cccgctccca tcgtggcgat gatggcgagg atcacgtcct tgcccgttac gcctgtgccg
    16141 agcgtgccat cgacctcgat ccgcatggcc ttcggcttgc gctgccagag cgtctgcgtc
    16201 gccatcacat gggtgacttc ggacgagccg atgccgaagg cgagcgcgcc gagcgccccg
    16261 tgggtcgagg tatggctgtc cccgcagacg agcgtgatcc ccggaagcgt gatgccctgc
    16321 tccgggccga tcacatggac gatgccctgg cgcttgtcgc cgatgtcgaa gatcgtgata
    16381 cccgccgcgt ccgcgttctt gcgcatcacc gagacgatat ggcgttgccg ggcgtcggcg
    16441 atgtccgcga ggttccgcgt tccggttggc atgtagtgat cgggcgtgcc gaagcttcgg
    16501 tccgggcggc ggaccttgag ccctgcgcgc ttgagccgct cgaaggcagg cagcgagccg
    16561 tcgtgcaaga ggtggcgatc gatgaacagg agggtgtagc cgtccggcct gtcgacgacc
    16621 acgtggcggg accagatctt ctcgaacatc gtcgatggcg cgggcatatg gcgtctacct
    16681 cgtgaaggcg gcgagaccgt ccgtccggag ccggcggcac tcgggagatt agccaagtga
    16741 tccaaatgag gctagatttc gtgcagcgag acggattcac tggcagggat cgtaggagtc
    16801 cggcgtccga accgatggga gcaacgtgaa tggccaacgt tcgcctgttg atcatcgatg
    16861 atcacgccct gttcagaggc gcgctcgggc actccctcag cgccactccc ggccggatgg
    16921 ccatttcgga ggctggatcg ctcgacgagg ccatggccca gctcgaggcg ggcaaggaat
    16981 tcgacctcat cctgctcgac ctcacaatgc ccggggtgca gggcctttcc gggctccttt
    17041 acctgcgcac cgagcatccg ggcattccgg tggtgatcgt gtcggcgagc gacgatgtcg
    17101 cggtgatccg cagctgcatg gagttcggcg catccgggta tgtgccgaaa tctcagccca
    17161 tcgagaagat ccgcgaggcc atcgaaagcg tgcttgccgg ccaggtctgg acgccgcccg
    17221 gactcgaact cgacggagcg tcctcgccgt tgggcgatct cgcctcgtcc ctctccacct
    17281 tgacgccgca gcaggtgcgc gtcctcatga tgctcggcga ggggctgctc aacaagcaga
    17341 tcgcctacaa gctcggagta tcagaggcaa cgatcaaggc gcacgtctcc gccatcctcc
    17401 agaagctcgg cgtcgacagc cggacgcagg ccgtcatcgc ggtcaataaa ctcaacgccg
    17461 gagcactacc gaagccggtc atgtgatttc cagagccacg catgccacgg agcgggccac
    17521 ccctcgccgg gcagccggag gcggatcgcc gcatttcggc agcgcctgca acccgctaga
    17581 cagtcgaaaa gtgcacggaa cgtaaacgtc aacctgattg actgcgtccc ggcgatcgac
    17641 aattcatggg cgatgcgata cggccgaggc tggcgtcgat cgacctcgtg ccagggtggc
    17701 aaatggacgt tcaggacatg cgcatatttg cccgcgtcgc ggcggtgcag aatttgtcgg
    17761 ccgtcgggct cgagctcggc ctcactccag gaacgatttc gaagcggttg caggcactcg
    17821 agaacgacct ctcggcgcgg ctcttcgatc ggacgactcg ctcgatccgc atcaccgagg
    17881 agggcgagat actgcttgcc cacgtcgagc gcatcctgag cgagctcgac caggcacgag
    17941 ccgcggtgag cggcaacgtc agccggccgc aggggcgcat caagatcgcc gcctcggccg
    18001 cgctcggccg gcgcctgatt gccccggcta tcagcgcctt catgcgccag ttcccggaga
    18061 ttgacctcca cgtcgtcatg accgaccggg ccgtcaacct ccaggacgaa ggctttgacg
    18121 ttgccatcag gatcggggaa ctcgacgatt cgagcctgat cgccaagcgg ctcgccgccg
    18181 acaagcaggt gatcgtcgcc gccgaggcct atctctctcg tcgcggggcg ccccagaagc
    18241 cggccacgaa tgcctcctcc tcgaggatca gtggcagtgg acgctgcgcg gcaatggcga
    18301 cgaccacgtc gtccgcctcg gtggcaggct gcgctcgagt gacggcgagc ttctccgcca
    18361 tgcggcgctc gcgggtcacg ggctcttgcg catttcggaa gtcgccgtcg ccgacgactt
    18421 gcgcagcggc ggcctgaagc gcgtcctccc cgactacgag atcctgaccg ggtcggcgat
    18481 ctgggcgatc tacccgagca gcaagttcat tctgccgaag ctccgcgtcc tcctcgattt
    18541 cctcggcgat cgcttccggg agaccgacat cgagtgcagc aacgacgccg aggatcgcca
    18601 tgccgtcccg ccgcgaggtt ttggcgctcg ctccatggcc actcgtgcgg ttgccgggca
    18661 ggcatcgggc ggctgaggcc gcctcgctac ccgctggccg cgaatgctgg taggagtagc
    18721 gagtgcttgt agcgttcgga tcgggtccag aacggatgtc ggcatggcgc atatcggagc
    18781 agttgagttg agtgccggcc cagccgccgg gacggcgccg tgacgagcgg gccgcgccag
    18841 ggcggttttc gcccgctcaa ggtggtcgcc aaggtcgcgg aaagcagctc ggtgacctcg
    18901 ttccatctcg agccgctgac gacggcggcg tggcaagcct ttgagccggg acagtttctc
    18961 gtcctgcgcg ttcccgccgc caatggccgc cgcgccatgc cccgcacata cagcgtgtcc
    19021 tggtatccag gggccggcgg gcgctatcgc atctcggtga agcgcgaggg cgcgccgaag
    19081 ccgggtgtgc ctgacggcgc cggctccagc tggctgcacg acgctgtcgg cgtcggcgat
    19141 accatcgaag cgagcggtcc gcggggcgaa ttcgtgctcg accggacgag cgaccgcccc
    19201 gtagcgctca tttcaggcgg cgtcgggctg acgccgctcg tcgccatgct gcatgcgctc
    19261 gccggcgagt ctcggcggcc cgtcaggttc atccatgcct gtgaaaacgg cgacgtgcat
    19321 gccctccgcg acgaggttct ggcgttgtcg cgctcacgcc cgggcatcga tgtgcacttc
    19381 tgctaccggg tgccaacggc gcgtgatctc gccgcggccg gccaccacag cgtcggcctg
    19441 ctgaccggcg gacagatcga tgcgctgctc gccacggctg acatggacta ttacctatgc
    19501 ggcccgccgc ccttcatgaa ggccgtgtac cgcatgttgc gtcagcgcgg cgtcgccgcc
    19561 gatcgcatcc gctacgagtt cttcggtcct gcgaccgtgc ttgatgagga tgtagctgcc
    19621 gctccgccgg cgattgccgc agagtcgcag tcctccccgc tgctcgcctc cgcgtcggtt
    19681 ccgtctcccg ccatcaccgt cgaattccgc aagtcgggca ggatcgtgcc ctggacggcc
    19741 gacgccaagt ccattctcga ccttgccgag gctgcaggat tgtcacctga cttctcctgc
    19801 cgtgccggcg tctgcggaac gtgcaggacg cgcctcttgg ccggggaggt tcgatacttc
    19861 gagcctccct cgacgaaccc gggccaggca tggcgctcat ctgctgttca cgtccaaaag
    19921 gcccggtcgt gcttgacatc tagggtctgc aaattggtaa gaccatcgta ccgagacggt
    19981 cgtcgcaact gatccggccg gagtgacagc ggcgagacga gttctcgatc gcgcgggcga
    20041 gcggcccgtc gacaggacac cacgaatttc aaaagatggc aggggagcat cgtcagtgaa
    20101 ggttctggtc acaggcgccg ccggattcat cggctccgcg acggcagccg cgctcctcga
    20161 tcgcggcgac gaggtgatcg gcctcgataa tctcaacgcc tactacgacg tgagcctcaa
    20221 ggaggcgcgg cttggacgac tgacggggcg ggccggcttc tcgttccatc gcctcgatct
    20281 cgagaaccgt gcggccatcg ccgacctctt cgcgcgcgtc cgccctgagc gcgtcgtcca
    20341 tctcgcggcg caagcaggcg tgcgctatgc ctcggagaac ccgcacgcct acgccgacgc
    20401 gaatttgaca ggcttcctca acgtcctcga gggctgccgc cacaccaagg ccggccatct
    20461 cgtctatgct tcctccagct ctgtatacgg cgccaacacc gcgatgccct tcagcatcca
    20521 ccagaacgtc gaccatccgc tgagcctcta tgccgccacc aagaaggcga acgagctgat
    20581 ggcgcattcc tattcgcatc tctacagcct gcccgtgacg gggctccgct tcttcaccgt
    20641 ctacggccca tggggccggc ccgacatgtc acttttcctc ttcacccgga agatcctggc
    20701 cggcgagccg atcgacgtct acaatcatgg caaccacgcg cgtgacttca cctacatcga
    20761 cgatatcgtc gaaggcatcg tacgcacgct cgatcgtgtg gccacaccca atgccggctg
    20821 gtccagcgac gcgcccgatc cgggtacgtc cgcggcgccc taccggctct acaacatcgg
    20881 taacaacaat cccgtcgagc tcatgcactt catcggttgc atcgagaccg ccataggtcg
    20941 cgttgccaag aagaatttcc ttcccatgca gccaggagac gtgccgaaga ccttcgccga
    21001 cgtcgatgcg ctgatcgaag acgtcggctt caagcctgcg acgccgatcg agctcggcgt
    21061 cgcccgcttc gtcgcctggt atcgcgaatt ctacaaagtc agctgaacgc agtcggcttc
    21121 cgtcggcctg agcaggagac gccgtcatgc cgcttgcgga catcggaagt gtgaagattg
    21181 ccgtgatcgg cctcggctac gtcgggctgc cgctcgccgc ctacatggcg cggcacttcc
    21241 ctgtgatcgg attcgatatc gacgcggaac ggatcgccga gctgtcggcg ggccacgacc
    21301 gcacgcgcga ggtgaccgcc gacgagttcg ccgcggcacg cgcgctgcgt ttcagcgcgg
    21361 acgcggcagc cctcgagacc gccaacttct tcatcgtcac ggtgccgacg ccgatcgaca
    21421 ccgcgcgccg ccccgatctc accgcgttga tgcgggcgag cgagaccgta ggtaacgcca
    21481 tccgcgacgg cggtgtggtc gtattcgagt cgaccgtcta tcccggctgc acggaagagg
    21541 tatgcgttcc catcatcgag cgcctgtcgg gtctcaagtt caatcgcggt ttcttcgctg
    21601 gctactcgcc cgagcgcatc aatccaggtg acaaggcgca ccgcttcccg gacatcctga
    21661 aggtcacttc cggttcgtcg gcagaggccg ccgcgctgat cgacagcgtc tattcggccg
    21721 tggtcagcgc gggcacgcac aaggcgagtt cgattcgcgt cgccgaggcc gcaaaggtca
    21781 tcgagaacat ccagcgcgac gtcaacatcg cgctcgtcaa cgaactcgcg atgctgttcg
    21841 acaagctcgg gctcgatacg cgcgaggtgc tcgccgcggc cggcaccaaa tggaacttcc
    21901 accgctacac gccgggcctc gtcggcggcc actgcatcgg cgtcgatccc tattatctga
    21961 cccacaaggc gcagtcggtc ggcttccaca ccgagatgat tctcgccggg cggcgcatca
    22021 acgacggcat ggcgacctgg atcgcgcaca acatcatcaa gacgatggtg cagcacaagg
    22081 tgagggtcga cagcgcgcgc atcctgatca tgggtttcac gttcaaggaa aacgtgcccg
    22141 acacgcgcaa caccaaggtc gccgacctcg tcacggagct ccaggatttc gccgccgacg
    22201 tcgtgatcta cgacccgcat gcaaactcgc gcgaggcgga gcacgaatac ggcctcacga
    22261 tcgccaacag cctgccgggg ggcccgttcg aggcgatcgt catcgcggtc cgccacgacg
    22321 agatcgtggc actcagcgcc gaacgcattc gtgccctgct tgcgccgggc ggcatcatct
    22381 acgacatcaa gggcgtgctg ccgggcagca gccgcactgc cggtctttga tatccatggc
    22441 acggcccaga cctgatcggc aatttcgcgc ctacgcgcga gctcccgaag acgacttccg
    22501 cgctctccgc ggccgcttgt ccttggcagg cgcggcagcc tcgcgatcac ccgatgccgc
    22561 cgcctctgcc acgtaacccg gcagcgcgaa cgcgccgcga tgaccggccg gcgtccagta
    22621 gcgcgtggtg atgccgcgct tcttcgcacg ccgcgcgagt ttcttgcgcg agaccttcag
    22681 gtggctcccg tcatcggagg cccagttgag cgcaaaaggg ccgccgaagt aacagggctg
    22741 ggtgcacagg tagggcgcgg cgttggcgaa cagctcctgg aaatactccg tcgtgcggcc
    22801 gaggtgggcg gggtggtgga acggcaagcc gttctgcgtg acgagaatgc cgcccggctt
    22861 cagcgcgcgc ttgcagtccg cgaagaattc gcgcgtgtgc agaaccgcgc tcggaccgat
    22921 cggctcggag ctgtcgacaa tgatcgcgtc gaagcgccgg tcggtatcgg cgacaaactt
    22981 gagcccgtcg gcgatgatcg tctccgtgcg cttgtcgtcg aaagcgccgc cggcgatctc
    23041 cggatagtgc tcgagtgcgg tatcgatcac ggtgcggtcg atctcgacga gaactgcgcg
    23101 ctcgaccgaa gggtgcttca gcacctcgcg cagcacgccc ccgtctccac cgccgatgac
    23161 gagcacgtcc ttcggcgacc tcatggccat cagtggcacg tgcgccatca tctcgtgata
    23221 gacgaactcg tcagacgtcg tgagttgcgt cacgccgtcg agcatgagga ccgtccccca
    23281 ggtctcgttg gtgaatatga ccagatgctg atgctcggtg cgcacctcgt gcaggatcct
    23341 gtccgccttg agccgcacac gccagtgcgg atggaacgtc tcctcgatcc agcgatccat
    23401 gccgggcctc catcgctcga agcttgacga aaagaaacgg accacgtgcc aaccaatgag
    23461 cacgcggtcc gtcgattgca cgcagcgctg atgccgtcag ccgacggccg ctgccttcaa
    23521 gcgaaccggc gccttcttgg ccgaggccgc gcgccagttc atctcgtcga gctcctcgcc
    23581 gcgacggtgg accttgacgg ccacgtcacg tgcctggaac tcgcgcttca ggagatcgac
    23641 gcacagctcg ggcttggact gaccacacat gaagacatcg aaagcggcat agtccgcctc
    23701 cggccagctg tgcaccgaga tgtgggattc cgaaagcacg gcaacaccgg acacgccccc
    23761 attcggcgtg aagtggtgca gatggatgtg cagaagcgta gcgcctgcaa cttcgacgca
    23821 tcgtcgcaac gcgcgctcga tatgcttgag gtcgtccaac ctcttggcac cgaacagatc
    23881 aatgatcacg tgggtgccag caaatcgaac gccatcacgt tcgatgaaga agtccttcct
    23941 gtcctcgtgc gctacgcacg cgtatgcagc atcatcttcc ttttgggcgg tgcttgaacg
    24001 agtcaagtcc atccccaatt ggaagagggt gtcatcgtag gccatatgac cctccccaac
    24061 cagtagacag aggtgacccg cccggctcct tacctctttg gttttgggga ttgtgtcctt
    24121 ggaccgctgt ggcaggtgga cgaacggaga gccgtgcata tcgtcgatat gacccccacg
    24181 cgcaagagga aactttgccg ctggcccaaa aatagttttc accacggtag cgatgcctta
    24241 accgatccgg gctcgacact gatcgcagcc gctctcaagg atgggacgat gacgcagcaa
    24301 aggcgggcca gcgcgctcga cttcatccag catgaggccg caggtggcat tgcattggtc
    24361 gtcgcagcga tcgcggcgct gttgatcgcg aactcgccgc tcgcatccga ctacaaggcc
    24421 ctccaggaga ttccggccgg cgtccgcatc ggcgccatcg gcctgcagaa gacactgctg
    24481 cactgggtca acgacggctt gatggccatc ttcttttttc tcgtcggtct cgagatcaag
    24541 cgcgagttcc tggtcggcga actctccgac cgccaaagcg cggcgctgcc gctgttcggc
    24601 gcactcggcg gcatgattgt cccggcagtc atctacgcaa tgatgacgta ccgggattcg
    24661 agcatgctgt ccggctgggc catcccgtgc gccaccgaca tcgccttcgc cgtcggagtg
    24721 ctggcgcttc tcggcaagcg gatcccgccc gcgctcaagc tgtttctcct cgctctcgcg
    24781 atcatcgacg atctcggagc catcatcatc atcgcgctgt tctatacggc cgacttgtcg
    24841 gctatggcgc tcgtgcttgc agccttcggc atatttggcc tcgtcattct caatgttgca
    24901 ggtgtcgccc ggctcggcgc ctacgtcctg cttggcgcat tcgtctggat ctgcgtgctg
    24961 aaatcgggag ttcacgccac ccttgccggc gtcgcgaccg cacttgcatt gccgctcggc
    25021 gtagcgagcc gggaagaaga tggcctcgat gggcacgcag gcgggctgca ggcggacctg
    25081 catccctggg tcacatttgc catcctgccg ctgttcgcct tcttcaatgc gggcgtctcg
    25141 ctctccggaa ttggcgtcga tcaagtggcg agcggaatta cgctcgggat cgcgctcggg
    25201 ctctttgtcg gcaagccgat cggcatcgtg ctctttaccg ctctggcgat caggaccggc
    25261 cttgcgcgtc tgccggatgg cgtttcctgg cggcacatgg tcggctgcgg cgtcctttgc
    25321 ggcatcggct ttactatgag cctgttcatc ggaacattgg cgttccccga cggatcgcgc
    25381 gaggtcgagg tgcggctcgg cgtcctgctc ggatcgctcg tttcggcagt ggtcggtgga
    25441 ctgatgttat ggcggtgtgg ccgcaacacc gaagagtttg agcaatcctg agcgggcagc
    25501 gcctcaccgc gacgtccgcg gaggcgtttc accgatcgtg cggcggatga gctcctccat
    25561 cgtctgggga ggcgtgtcgg cgcgaggcgc gtcagcacgc ttcttcgcca tgttgagcgg
    25621 cgggacgatc gccgttcgcg taggtacggt gacgaccggc gcggtcgacg ccggcatcga
    25681 ggcggcggtc ggacgcgctt tcggaggtac gacgcggccg gccgccagca gtgcgtcgag
    25741 gccgaccgtc gccacgcgga ggtcgacgtt gaccggtgtc ctctcgcgcg tgaggtcgac
    25801 cgtgatcgtt cccttgccgc tcgccttgtc gactttgatc tcggcgtcgc ggaccgtcag
    25861 cgagcgctcg ccgacgacaa ggcgacccgt gatcgagacg ggcgcccgga gcgatggcgt
    25921 ctcgacgccg agccaatccg ccagcgcgtc cggcaaggcc gagcgcagga gcatgctgcc
    25981 atcggcgtag cttgcagccg ggcccgggac gaccttggta tcgccgagcg tgccgctgaa
    26041 gctcgcctca ccgaggcgag aggcggccga cacactgatc cgctcgggct ggcgcgtgcg
    26101 ggaggcggct agtccgtgga ccgtcgcctt gacggtcacc tcctcgccgc ggaacacgcc
    26161 gagggcgcgc atctcgagcg gtccccgctc gctcgcgcaa gtcgccgtgg ccctgatccg
    26221 gctcgcctcg taggcaacgc cggtacgcga atccatgtac cgtagcgtgc cgtcgacgat
    26281 ccggatttca ccgatgtgcg ggcgcgtgcc accgcgtggt gccggcgggg cggtgacgac
    26341 agatgccgta tcgctcaggc tgcggccggg aaccgtgctg ccgcctgcat ctacgctcgc
    26401 aattcgcacg ggcccaccga caactccaat gtcccacgac cgctgaccgc gactgtcgac
    26461 caccagttcc atgaccggcc gagtcaggat caggtgggcc acgtccaagc gcccgagcag
    26521 cacgctgatc agacctgtct cggcctcgat ttcggccacg cggacgagcg gcggcacgtt
    26581 catgtccgaa ggcgacgaga gcgtcacatc ggccagcctg atcgacagcg atgggaagag
    26641 actgacgctc gtcggcccat tgaccgacac cttgcggcct gtccgcgcct cggcctcggc
    26701 aatgaggcga cccttgatga actcggtcgg gaccgtcgtc gcggcaagca ccgagagcga
    26761 ggcgaccagg gccgccggca atccgagacc gaccaccaaa gcgcgccgcc agcggcgcgg
    26821 ccgttccggc tccagctcga cctcgtcgtc gagcgaaact atggagcccc ggctgaggcg
    26881 ccgcgccatg gcgatgtcgc gatcgtccga catctcgtcc gcgctcgcgt ctcgatcgtc
    26941 cggcccaggc agcgacagct cgtgggtgac gtcccgcggt tcggcactcg acttgtcact
    27001 cgcactcatc tgctaggtcc cgtcggcctc ccccgcacgt gcaactaccg cgtcaatgtg
    27061 tccgaggctg gcccgcatca tgaaaacgcc gcccacggac ctgcctcatt cgttaacgtc
    27121 agtagacgtc cgcgtgcatc gtcgccatga acgaggggcg ggccgcgatc ttggcgtgcc
    27181 aggccgcgag cttcgggtgc gaggcgcgcc acccggcttc tgccgggaac cggaagtcga
    27241 ggtagccgag cgccgtgccg atcgcgaggt gcccgatatc gatcggcatc gcctcgatgc
    27301 gtgcagcatc cgcttcgagc gtcgccagca cggccgacag cttgagccgg cacccctcga
    27361 ccagcgcctg ttgctgttgc tcggccggct tcacgcgctc gagcacccag agcagcagga
    27421 cgtccatcat gccatcgccg agcgcgttga gccggatcgc atcgaggcgc cgcgccggat
    27481 cgcgcggatg gagcgtcccg ccggcgagac cgtcgagata ttcgcagatc acgcgggaat
    27541 cgtagacggc tctgccgtcg tccaggatca gcgtcggcag cttggcgagg ggattgtact
    27601 tcatcacctc gacgttcggc ttcgtcgccg agacgatcac ccgttccgtc gcgatgcggt
    27661 ccaccagtgc cagctcgtgc gcgaccacca tcaccttgcg cacgaacggc gagcgcgacg
    27721 accagaagag tttcatgcga gcctccttcg cgacagatcc gggtgaccgc atgctagcgt
    27781 cggctctcag gtgccgtaag cgcaattgca cgcgaatcgg gtgtcgcggt gccggcacgc
    27841 atgagaagcg tcatgagcat ggagcgccga aggagcaaag gccgtgaccc gaaaagcact
    27901 cgctatgacc gaagacggcg acgtcgcgca cgcgcgcccc gatgaccctc cgtcggcggc
    27961 gatcgattgg gcctccattc aagcaggcct cgacacgtcg ggtttcgcaa ccatccgagg
    28021 tctcctgccg gcacgggcct gctcagcgct ggccggcctc tacgacacag acacgcactt
    28081 tcgcagccac atcaacatgg cgcgccacgg cttcgggcgc ggcgagtaca agtattttgc
    28141 ctacccgctc ccgccgatca tagccggact tcgcgcgatg ctctatgccg gcctggcgcc
    28201 gatcgccaat cgctggagcg agcagctcgg cagcgacacg cgctatcccg agacactcgc
    28261 ggcctatctc aagctttgcc acgccaccgg ccagacgcgg ccgacgccgc tgctgcttcg
    28321 ctatcgctct ggcgaccaca actgcctcca ccaggacctc tacggcgatc tcgtctttcc
    28381 gctgcaggtc gcgaccctgc tcgccgagcc cggcgcggat ttctcgggcg gcgagttcgt
    28441 gctgaccgag cagcggcccc gcatgcagtc gcgcgtgcgc gtcgtgccac tccgccgagg
    28501 cgacgcggtg gtgtttccgg tgcaccatcg ccctgtcgag ggaactcgcg gcgtctatcg
    28561 cgtcaacatg cgccacggcg tcagcgaggt gacggcgggc gaacgccaca cgnnnnnnnn
    28621 nnccgcctga cgcacggccg gcgccacacg ctcggcatca tctttcacga cgggcgatag
    28681 ctatggccgc aggcgacttg tttgcatcga gcgctacact acatcggacg gagatcgcgc
    28741 cgggcgcagt gctgctgcat ggctttgccg ccgaccgcgc cgagctgatc atcgcggcca
    28801 tcgacgcagt cgcgagcgcg gcgccgttcc ggaacatgat cacgcccggc ggacacacga
    28861 tgtcggtcgc catgacgagc accgggcgcg ccggatggat cacggaccgg cgcggctatc
    28921 gctactccga gatcgatccc gacaccggcc gagcctggct cgcgatgccg gcaccgctcc
    28981 acgcgctcgc cgttgacgcc gccgccactg gcggctttcc gggctcagag cctgacagct
    29041 gccttgtcaa ccgctatgtg cccggcgcgc gcatgagcct gcaccaggac cgcgacgagg
    29101 ccgatctcac ggcgccgatc gtgtcggtat cgctcggcct tccggcgaag ttcctgttcg
    29161 gcggcctccg ccgcgccgac cgcccccagc gtctcatgct cgcgcatggc gacgtcgtgg
    29221 tgtggggcgg gccgtcgcgc ttggcctatc acggcatcga tcccgtgaag gatggcaagc
    29281 atccactcac cggccgctgc cgcatcaacc tcacattccg aaaagcgctc tgaccggcct
    29341 cgctccgttg atcagaccca ggcgagcttg ccgttctcga tcaccatgag cttgtcctgc
    29401 ggcacctcaa gatcggtgat atcgtgcggc gtgaggccga gcagcagccc gcgcagcacg
    29461 cggctcacgc cgccatgcga gacggcgatc gtcggctccc ggacgccctc gagccagcct
    29521 gcgacgcgag cggacagcat cgcgtagctc tcgccctggc ggggcctcca actccaggga
    29581 tcggctttgc gcccagcgaa cccctccggg tcctcggccg ggaggtcgta ccagacgcgg
    29641 ccctcccagt gtccgaagtg gatttccttc agccggtcat cgcggcggaa gccgtcaggc
    29701 tcgagcccga tctcgcggcg gatgatcgtc atggtctcgg cggtgcgcga cagggggctc
    29761 gcgacatagt cgagcgccgc ggccttcgag ccgagtagct ctttcagcgc acgcccgtta
    29821 cgcgccgcct ggccgcgacc gtgatcgttg agcggagtat ccgtttgccc ctggtagcgc
    29881 ttggcgacgt tccagtcggt ctcgccatgc ctgacgaagt agatgatcgg catcttgacc
    29941 atcatcagac ctcgagcacc agcttgccgt cgttgccgcc ggtgtagagg aggttgagca
    30001 cgtccggata ggcgtcgatg ccgcctttgc gcatgtcctc acggatcacg agcttgccct
    30061 ccttgtgcca ggcgccgagc tggctcacgg cctccgggat gcgcgcgttc caatcgaaca
    30121 caatgaggcc ctgcacgcgg agccgctgcg tgagaatcgc gcggaggttc ttcgggcccg
    30181 gcaccgcctc cgtcgcgttg taggccgaga tcaggccgca aacagggatt cgcccgaagg
    30241 tattcatgag cggcagcacc gtatcgagga tctcgccgcc gacgttctcg aagtagacgt
    30301 cgatgccgcc agggcaagct gcctcgaggt cgcgtttcag gttgccggcc ttgtagtcga
    30361 ccgccgcatc gaagccgagc ttgtcgacga gggtacgaca cttctcgggt ccgcccgcga
    30421 tgccgaccgc gcggcagccc ttgagcttgg cgatctggcc gacaatcgag ccgacggccc
    30481 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgagg aggccgaagt
    30541 aggccgtcca acccggcatg ccgagcccgc cgacccagcg ggccatcggc gccgcgccaa
    30601 gatcaatctt cacgacccgc tcgcccttcg agatcgcata gcgctgcacg ccgaacaggc
    30661 cctgcaccgc gtcgccggac ttgaacgacg gattgttcga cgcctcgaca atgccggccg
    30721 cataggcgcg catcacctcg ccgatgccaa ccggcttcac gtaggatttg ctgtcgttca
    30781 tccagccgcg cattgccgga tcgagcgaga tgcagccgac cttaacgcgg aactcgcccg
    30841 ggcccggctc agagatcggc gcgtcctcaa tcgtgaagtc tgatcgctgc gccatgccgc
    30901 gcgggcggcg cgcgagcttc accattcggt tctgctctgc catgtctcgc tccttgcctc
    30961 gatcgttcac ttgtcgtccc ggatggttct tttccacacg acgtcgtccg gcgcaatcgc
    31021 gctgcaagaa gcccggcaac gcgacgccgc gcgacgcgcc ttgcaactgc atcactatgg
    31081 ctcattggtc cggccgactg cgccatgcga gcgtctccga tgtccctaac gtgcggtcca
    31141 aacaaatgtt ggcaacgcac gggacgcgtt ggccatcacc ctggatttcg ggcatacgca
    31201 cgcttgcccg cgccggtcag ccgcggcatc gggacagaaa cgtcgacgcc gaggcatgga
    31261 gaccatgagc catctcgaac agaacttggc gacgctggag gcggaggcga tccacgtctt
    31321 ccgcgaggcg gccgcgcagt tccgccggcc ggtgatgctc tattcgatcg gcaaggattc
    31381 gacggtcctc ctgcatctcg cccgcaaggc attccacccg gcaccgctgc cgttcccgct
    31441 cctgcacatc gacacgggct ggaaattccg cgagatgatt ggctttcgcg atcgcatcgc
    31501 ccaatcgctc ggcctcgacc tcatcgtgca cgtgaaccgc gaaggcgaga ccgccggcgt
    31561 ctcgccggtg acgcacgcgc cgtcggtcta tacggacgtc atgaagacgc gtccgctcaa
    31621 ggaggcgctc gaccgttacg ccttcgatgc cgcattcggc ggggcacggc gcgacgagga
    31681 ggcgagccgg gccaaggagc gcatcttctc ggtccgctcc gccggccacc gctgggatcc
    31741 gcgcaagcag cgcccggaga tgtttggtct cctgaacggc cgtctcggca gcggcgagac
    31801 catgcgtgtc tttccgctct ccaactggac tgaaggtgat gtctggcgct acgtcctgcg
    31861 cgagggcctc gagatcgtgc cactctatct cgccgcggac cggccgacca tcacgcgagc
    31921 tggccagatc ctcgtcctcg atgacgagcg cctgccgctg ctcccgggcg aggcggtcga
    31981 gacgcgtcgc gtccgcttcc gcacactcgg ctgctggccc ctgaccgcag ccgttgcttc
    32041 ctccgccgac acgctcgaag ccgtcgtccg cgagacgctc gccgcgcgca gctccgagcg
    32101 gcagggccgc ctcatcgaca aggaccagtc gagcgccatg gagcgcaaga agctcgaggg
    32161 ctatttctga tcatggcggc gtcggcagat aaccgggcgg atagtgaccg tgctacaagc
    32221 gccggcgcat cgcgcgccat ggtgcgcgtc ctgacctgcg gctcggtcga cgacggcaag
    32281 tcgacgctca tcggccgcct gctctgggat gcggctgacc tccccgacga cacccgcgcg
    32341 acgatcatgg cctcggccgg cgccgacggc ataccggacg tgagcctgct cctcgacggc
    32401 ctcgaggcgg agcgcgcgca gggcatcacc atcgacgtcg cctggcgcta cttcgacctg
    32461 gcaagccgcc gctatgtcat tatcgactgc ccgggacacg agcagtacac gcggaacatg
    32521 gcgaccggcg cctcccaggc cgacatcgcc atcatgctga tcgatgcccg ccagggcatc
    32581 aagctccaga cgcggcggca cgcggcgatc ctcgagctcg tcggcatcaa ggaggtcatc
    32641 ctcgccatca acaagatgga cctcgtcgat tggtccgagg cggtctatcg cgcgatcgcg
    32701 gacgagtttg cggcgctctc cgcgcgctct ggcttcgagc gcgcagtcgc cattccggtc
    32761 gcgagcaagc ccggcgacaa catcgcgcgg cgatccgagc gtcttcgctg gttcaacgga
    32821 ccgacattgc ttgacgcact cgaggcgacg tcgcggagcc ttgcgacggc gacgagaccg
    32881 ttccgcttcc cggtccagcg cgtgacacgc gccagccgcg atttccgcgg gctcgccggc
    32941 actgtcgcct cgggcggggt cgctgtcggc agtgaaatcg ttgaagtgct cagcggcaag
    33001 cgggctctcg tgaagcggat agcgacattc gatggcgacc agccgacagc caccgccggc
    33061 gatgccgtga ctatagtgct cgatcgtgac ctcgacatag cgcgcggagg cctactcgcc
    33121 tcgccgtcag agcctgcgac agccgcgacc gatctcgaag tgcagctcgt ctggctcgcc
    33181 gataccggtc ccggacacag cggcctgctg tttcgaacga caaccgacct cgtgcccgtt
    33241 tccgagttcc gcgtcatcgc tcgcatggac ctgcaaacac tccagaaaac cgcggcggca
    33301 tcgtgcggtg ccaacgacgt cgcggacgcc cgcatcaagc tcggccgcgg cgttgccgtc
    33361 gacaggttcg ccgccgatca tgcgactggc cacttcatcc tggtcgatgc cctcacgggc
    33421 gcgacagttg cgggtggcat cgtggccgac atcgtgcagg acaaggaggc acgaagcggc
    33481 gacgtgttcc gcctgacgcg cgcgctcctc gagacgacgg tgtgcgccga cannnnnnnn
    33541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33661 nnacttcgac cactccgaag gcatgatccg gcggcggcag cgaatccgct gcacgccgcc
    33721 gcaagctctg ctcagctcgc tttcagatac cgggcgagcg cgtccaggcc atcgtcgctc
    33781 ggcggcgtct tgagcgcgaa ctggttgacg aacggcaccg gcaggtcgac gccgcgtttc
    33841 gcctcgatcg ggtggatctc accggagccc gtctggattt cgacgagctt cgtctcgaac
    33901 gagccatcgc tgtagagcac cgtgatcttc agtctgaggc tcacgctgtc caccggccgc
    33961 tcgccgatca gcagatcgcc acgcggacga tcgagccagg ccggcagcgg ctgaccgtcg
    34021 gcgagcatca cgcgatactc gctcacggtc tttgcttgat ccatcgccat gttcgagatc
    34081 tggatcatga gcatgcgctc gcgcactagg gtctggatga tgagttcggt ctgcgacccg
    34141 tcgccgatcg tgtgaccaag aaggctcgtg cggagcgaga aaccggtaag gccctccggc
    34201 atgcccgtgt tgtcgccgcg gtggaatccg aggcctgccg agccaagcga ccgctggatc
    34261 tcccagatgc ggttcgaatt gatcggactg ccaccgtcga caacgccgat gtaacccgaa
    34321 ccggagacgc catccagcga ggcgacctga ttgaccgcag cgaccacgat gccgtgactt
    34381 ccgaagtcgc cgatcaaccg gcctggcccg ccgatccgct gcaccgcatc gatgaccatg
    34441 ccgtcggccg caaggggctc ggcagcctgc gcggctcttg gagtatcggc cggacgggca
    34501 gagtccgcag ccggcggcac gacgtccagc acattgacgc gaaccgtcgc gatgctgctt
    34561 ccgccttggc cgtccgagat cacgtaggtg atcgtcgtct cgccggcgaa cccgccattt
    34621 gccgtgaagg tcagcgtgcc gtctggattg atctccacgg tgccgcccgg actgtgggcg
    34681 gcgatgacgc ggagcgtatc cccgtcgatg tcggtgtcgt tgccgagcac gttcacgcgc
    34741 gtcggaatgt gtggcttcgg ccacgtcatc acgagcgatc gggtcgtcgt tcaccggcga
    34801 gacgggcccg agcagcagcg cccccgtgtc ggtcgcaacg ccgtcggaga cagtgtaagt
    34861 caccggcggt acaggtccat tgtagttctg cgccggcgtg aaggtgaagc ttccgtcact
    34921 gcgaatgacg agctcgccct cagacagtac gaccgtggtt ccagcggtgt aggtcgtgcc
    34981 g
//
LOCUS       DAORNH010000012        34486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_963_length_34486_cov_2.676512, whole genome shotgun sequence.
ACCESSION   DAORNH010000012 DAORNH010000000
VERSION     DAORNH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 34486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34486
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_963_length_34486_cov_2.676512"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="PK264_02395"
     CDS             complement(<1..1034)
                     /locus_tag="PK264_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRD74778.1"
                     /translation="MSPRRPDLTKLLAPRSVAVVGAAPIGQGIRGRALEVLRMHAFAG
                     AVHPVSRSLAEVQGLKAYPSVAALPETPELAVLVIPAANVPDELERCGKAGVGAAVVI
                     SSGFAEEAGEAGRASQQRLRAIADRYGMALLGPNSEGFATLGAKLCVTFSPAVADTSL
                     PLIPEGKRGRIAVVAQSGGMGFAFYDRGRPKGLAFSHVVTTGNEAQLEVFDVVEHLID
                     RDEADVFLLLVEDVKTAATFVRAGRKAQAAGKPIIAVKIGKSDAGQRAAKSHTAALAG
                     SYAAFSAMARSLGMIEAHELDEAVDLAQAFLAWRQLPPKGRRVAICTASGGGGGWLAD
                     ASSAAGLEVPL"
     gene            1236..2477
                     /locus_tag="PK264_02400"
     CDS             1236..2477
                     /locus_tag="PK264_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD74779.1"
                     /translation="MSQPTVLFRNARLLDGRADSPLDDHDVLVSGGMIREVSGKRLSA
                     EGAMVHDLAGRTLMPGLIDCHVHVIATLANLGANAELPNSLVALRSAEIMRGMLLRGF
                     TTVRDLGGADLGLRMAIEEGTIVGPRLVICGKALSQTGGHTDYRGRYHSRSVDYYRDR
                     LGTLGRVVDGVDEVRRVCREEIKGGADFIKLMANGGVSSPTDPIKFFGFSRTEMLAAV
                     EEAQSAQTYVAAHLYTDEAIRRAAECGVISIEHGNLIKPDTAKYLKEKGAYVVPTNVT
                     FDVLAKEGAAMGLPPESVAKIEDVRSAGLSALKLLAEAGVMMAFGTDLLGPMHHHQSD
                     EFLIRARYLPAIEVIRSATVNAAKVVGQEGKLGVIAPDAHADLIVIDGDPLKDMNLLV
                     GQGRHMAAIMKGGSFVKNALK"
     gene            complement(2529..3935)
                     /locus_tag="PK264_02405"
     CDS             complement(2529..3935)
                     /locus_tag="PK264_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HRD74780.1"
                     /translation="MKRLLYATAAVGLAIAAPMDRAEAFCGFYVAKADTKLFNKASKV
                     VVARKGDRTVITMASDYEGAASEFAMVVPVPTVITKGQINITENALVDHLDAYTAPRL
                     VEYFDPDPCNIPRPMAMAPGATAARSADAAAREDRRRSLGVKIEAEYTVGEYDILILS
                     AKESGGLETWLRENDYKPPQGAGPVLASYIKQGMKFFVAKVNLKEKDRLGTKYLRPIS
                     VAFESPKFMLPIRLGTVNANGPQDMIMFFLTEKGRVETTNYRTLKLPSDMDVPIFTRN
                     EFGAFYKAMFETQVKKDGMKAVYLEYAWNMAWCDPCAADPLPNDKLVELGAFWLLDGP
                     GAPSGPGVMPRRMRPPGGPANVFVTRLHVRYDAVNFPEDLMFQETADSSNFQGRYVLR
                     HPFTGPATCSAGEAYKRNLQTRFQKEAATLANLTGWDIAMIREKMAKAGQPMSAPPIP
                     GGDDDAWYKKMWPGRSSP"
     gene            complement(3964..4848)
                     /locus_tag="PK264_02410"
     CDS             complement(3964..4848)
                     /locus_tag="PK264_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRD74781.1"
                     /translation="MFGASSRLQSRSLPTSAGPGSRCLLATVRTLDPRYLQIASLGIL
                     LTFGLGWLGFDQSLINVSVILATILATQLVLGWIMQPGRAFDPLSALISGLSLCLLLR
                     AGSLWVLALGAILAIGSKFFLRVDGKHVFNPTNFAIAVLLLFTDLAWISPSQWGSQTW
                     AAFLFACLATMVLSRARRGDVALAFLGTYVAVLFLRALWLGDPWAIPMKQMQSGALLL
                     FAFFMISDPKTTPDRRGHRILYGVLVACLGAWIQLGLYNPNGLMYALFFLSPLVVLMD
                     RAAPVASTDRYAWSRPSI"
     gene            5068..5685
                     /locus_tag="PK264_02415"
     CDS             5068..5685
                     /locus_tag="PK264_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRD74782.1"
                     /translation="MRFSLRLAIVALAAFALGGAVALLVLPGAREALFNPVRTVVTGA
                     ATIGGPFTLTDHTGKRVTDQDFRGRYMLVFFGFIFCPDICPGSLQVMTEAMERIGPKQ
                     AGKITPLFISVDHERDTPAELKEYVKSFHPRLVGLTGSATDIAAAAKAYRAIYRRVAD
                     QKSKAGYTIDHTTFFYLMGPDGKFITHFTHGVLPDVLAKRLAALP"
     gene            6345..7043
                     /locus_tag="PK264_02420"
     CDS             6345..7043
                     /locus_tag="PK264_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HRD74783.1"
                     /translation="MSKARMKCHVLVDFDGTIATVDTTDLLLEQFADPSWQEIEADWK
                     SGRIGSRECMIRQIDLVRASPTEMDAFVAGIEIDPGVPEFIRLCTSLGHRVSVVSDGL
                     DRTVGAVLRRHGIDVPHFANHLEHVGERRWRLTFPWARDDCKPLSGNCKCQFATSTTR
                     GLRDIRVVVGDGRSDFCVAEGADLVLAKSSLTRHCVANDLPHFAFTTFAEATDLFARW
                     LDQRVVANGRPFEQ"
     assembly_gap    7067..7088
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7095..8471
                     /locus_tag="PK264_02425"
     CDS             7095..8471
                     /locus_tag="PK264_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD74784.1"
                     /translation="MTDEELLALEARYCSHGDTVHYTNPPKIFTNCEGSYMYDSAGRE
                     FLDLQMWYSAVNFGYKNERIANAVRRQLDTLPQVASQYLHREKIELATLIAQDAERKF
                     GEKGRVHFNVGGSQAVEDSLKLIRNFKGGKQLMIAFEGGYHGRTLGASAITSSYRYRR
                     RYGHFSDRAMFIPFPYHFRGPKGMTKEEYGEKCVKDFARLFESEYYGLWDPKANECEF
                     AALYMETIQGTGGYVVPPMNFFKDLKKVLDQYGILLVVDEIQMGVYRTGKLWSIEHFG
                     VTPDVIVFGKAITNGLNPLSGIWAKEALINPKTFPVGSTHSTFNANPMGTGVALEVMR
                     MFAERDYEAMVMEKGRRFLAGLKDLQKRHKIIGEVDGLGMALRAEICEPHDSFTPAKR
                     IVDMMVDESLKGDLEVNGRKYGVVLDIGGYYKNVITLAPSLEMSHAEIDLALTLLDKL
                     MTRVTKAL"
     gene            8580..8945
                     /locus_tag="PK264_02430"
     CDS             8580..8945
                     /locus_tag="PK264_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74785.1"
                     /translation="MTPYALMFLALVLIGTTTGQLLFKAASVRAEGDGVGEHWRSLLT
                     DKLIWIAITIYILEFFIWMAFLSLVPLWQGVMVACADIVLVMVGGRIFFGEEITGARV
                     LAISLIAAGVLLVGLGGGK"
     gene            8942..9313
                     /locus_tag="PK264_02435"
     CDS             8942..9313
                     /locus_tag="PK264_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HRD74786.1"
                     /translation="MIRTTIIVGFILLISLDTYLQIAFKLASEATLPMTFDIEWFRRV
                     IRSPWTHSILVGYVFAFLIYMTILKHAPVGPAFAASHAEIVIVTIIGVLYFGESITFV
                     QAAGCALIVAGIGVLAATERG"
     gene            complement(9330..10055)
                     /gene="recO"
                     /locus_tag="PK264_02440"
     CDS             complement(9330..10055)
                     /gene="recO"
                     /locus_tag="PK264_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRD74787.1"
                     /translation="MEWTDEALILSTRPHGETAAIAELFTRARGRCVVLVHGGRSRKL
                     RPVLQAGNHVDATWKARLEDQLGHASVELRRGFAAEFMNDPAALAGLMSLAAICHLLP
                     EREPHPSLFEVAHFVLGYLDDADIWPALMVRFELALLGELGFGLDLTSCAATGATEAL
                     EWVSPRSGRAVSHMAGLPYRDRLLALPKFLIGSGGAASKEDIAAGFALTGHFLRARVY
                     APAGQELPAARARMIELVARRAA"
     gene            complement(10105..11043)
                     /gene="argB"
                     /locus_tag="PK264_02445"
     CDS             complement(10105..11043)
                     /gene="argB"
                     /locus_tag="PK264_02445"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRD74788.1"
                     /translation="MAKSGHEAAPAPKGVSDSAAGKARVLAEALPHMQRYDRQTVVVK
                     YGGHAMGDPALAEAFAKDIVMLKQAGVNPVVVHGGGPQIANMLKRLQLKSDFVYGLRV
                     TDKATVEVVEMVLAGQINKEIVAAINKQGGKAVGISGKDANLMIARKVTELPDPETGI
                     VKPVDIGFVGDPMAVNSEIIDVMTRSDLIPVVAPIGMSASGETLNINADTFASALAAA
                     IKAKRLLLLTDVEGVLDKDKKLLERLSVAEARALIRNGTITGGMIPKVEGCIEVVEAG
                     VEAVVILNGKVAHAVMLELFTEHGAGTMFGGEAGSA"
     assembly_gap    11156..11227
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11324..12235
                     /locus_tag="PK264_02450"
     CDS             11324..12235
                     /locus_tag="PK264_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRD74789.1"
                     /translation="MAVAEDIGQAPAARALAITLSRATHYAYAQGHRYITIEHALLAL
                     TEDAEACRLVMEAGGDVQRLRDDVAAYLGRLPDRLAPGERGPMIHPELTQIIDYASAA
                     ARRSPRPQVSGAVLLAAIIGEGRSVAASILTSQGLTFQSAIDALTRSTVAPLDPPAYA
                     QPDATGDLELPPWPASPTPSANPADVPAPPPYPDALPGLGSGAPLPTEPLLPFPSATP
                     APSGPMEQLLARARERVNAGRPVASEPPRGQSHLSRQPHLHLHCRTRRRSARYPLWRR
                     LPSPRTSAPCQNWSCRHSSRSDRPPPL"
     gene            12451..13167
                     /locus_tag="PK264_02455"
     CDS             12451..13167
                     /locus_tag="PK264_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74790.1"
                     /translation="MTAPAPWPDPAGLPPPGYAGTVPPTPADDNFAGAPYPDETIDQL
                     RAPTGTEPGGVRAPLGIAPGQLVENIPRAMRLALAHSVEVRIAREVVGNVASGMPGTV
                     TAALIMVTRSMSVRLRSPDGAFHIETISPETQWIDNRLGLMVDDYASWRWTVTPRRRG
                     TAELQLVVSARTVGDDGVVAETALPDQVVAVSVKVNWPLTLRRWGGWAGAMLAGGIIG
                     KLGEGGLAAGVSALRQAMGL"
     assembly_gap    13240..13276
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13395..14879
                     /gene="guaB"
                     /locus_tag="PK264_02460"
     CDS             13395..14879
                     /gene="guaB"
                     /locus_tag="PK264_02460"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRD74791.1"
                     /translation="MAHRPVEIRDDIGLTFDDVLLVPQGSEVLPGEADVSTRITKEIS
                     LNIPILSSAMDTVTEAPLAIAMAQAGGIGVIHRNLSPETQADHVARVKKFESGMVVNP
                     VTIHPSATLAEALELMARHRISGIPVVEPGRTGKLVGILTHRDVRFASNTRQLVSELM
                     TREKLITVREGVVRDEAKRLLHQHRIEKLIVVDDAYRCIGLITVKDMEKAQRHPSACK
                     DEQGRLRVAAATSVGDDGFARTERLIDAGCDVIVIDTAHGHSKSVLEQVTRIKRLSNR
                     VQVIAGNVATADGTKALIDAGADAVKVGIGPGSICTTRIVAGVGVPQLTAVMDAVEEA
                     AKRDIGVIADGGIRYSGDIAKALAAGASAVMVGSLLAGTDEAPGETYLYQGRTYKAYR
                     GMGSVGAMARGSADRYFQQDVKDQLKLVPEGIEGQVPYKGPAGAVVHQLVGGIKAGMG
                     YVGARTLTELNQRARFVRISPAAMRESHAHGVLITREAPNYPGG"
     gene            complement(14882..15076)
                     /locus_tag="PK264_02465"
     CDS             complement(14882..15076)
                     /locus_tag="PK264_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74792.1"
                     /translation="MADVIEFPVLERPSRPGPIVPRRGGSAEIVIFPGVRIERWTSIV
                     PTPPETPRAKRKLKRPALTD"
     gene            complement(15326..>15632)
                     /locus_tag="PK264_02470"
     CDS             complement(15326..>15632)
                     /locus_tag="PK264_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74793.1"
                     /translation="ALISALPAIPYLRFHDATTYAFVAAVAVFGLGAHYSMARAFSVA
                     DAIIVAPMDFMRLPIITVVGVMLYEEPLNPWVMAGAMIVVAANLINMWGERRRPAVR"
     assembly_gap    15633..15650
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15651..16254)
                     /locus_tag="PK264_02475"
     CDS             complement(<15651..16254)
                     /locus_tag="PK264_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD74794.1"
                     /translation="MATTDAGEDETNAVVPAPRRSGVVVALLWMTVALCCFTVIAIAG
                     REAQRRGMLTIELIFLRSVIALAVLIPILAATGQLVLAMRTGQPLLQIARGGVHWIAQ
                     FTWLYALALIPLAQLFALEFTAPLWVAVLAPLLIGERLTATRLAAAMIGFVGAIVVAN
                     PGEASFGRGALFACISALGFALSMIATRRLTRTDSAATILL"
     gene            complement(16281..17225)
                     /locus_tag="PK264_02480"
     CDS             complement(16281..17225)
                     /locus_tag="PK264_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRD74795.1"
                     /translation="MQRDIIVREVGTRDGLQLVKTMLPTEIKLAWIKAASAAGVPEIE
                     VSNFVPSKVIAQFADAADVARGSLAFPGSLMCALAPNRKGAEFALATGLKKLNFVMSV
                     SETHNLANVRKTSDQSFDEFRQIVELRGSRPEWKSVKIQGCLATSFGCTLEGKVDPDR
                     VLTFVERFLKLGADEILLADTVGYAGPSEVENLFHHTVTLATGKPVYAHFHDTRGLGL
                     ANVYAALEAGVGAFDASTGGLGGCPFAPGATGNIVMEDLVFLLEKEGLKTGVNLEAYR
                     EARAIAEKALPGERFYGGVPRAGLPKGFVPPSRVMAAE"
     gene            complement(17274..18341)
                     /locus_tag="PK264_02485"
     CDS             complement(17274..18341)
                     /locus_tag="PK264_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD74796.1"
                     /translation="MTAMDREIVSESERVHLEDIEVGRTLPFGKLSVSKEDIVAFARA
                     FDPQPIHLDEEAAKRSIVGGLCASGFHSCALQMRLLADGLLNRWTSLGSPGIDEVKWS
                     KPVRPGDMLTGRYTCTEKRALGSRPEVGLAKITMEMLNGSGDVVQTWFSNQLVKVRDP
                     KPVERAPEGAGSGTSARLESLWDGPPRPEPSRTSNYFEDRVIGETFDLGSQTLTREEV
                     IAFAREFDPQPFHLDDAAAAKTYFGRLSASGWHTAALFIRQFVAFRQKIEGEMRDKGL
                     KVAHYGPSPGFRNLRWLKPVYPGDTLTFRGRTAGKIDLKSRPDRGLIQTDSQARNQHG
                     EIVFAIRGQILAERRETYRPS"
     gene            complement(18507..18734)
                     /gene="rpmE"
                     /locus_tag="PK264_02490"
     CDS             complement(18507..18734)
                     /gene="rpmE"
                     /locus_tag="PK264_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRD74797.1"
                     /translation="MKKTEDFHPDYHQIKVVMTDGTEFVTYSTYGKEGDTLNLDIDPK
                     THPAWTGGGNQILDRGGRVSRFKKKFEGFGV"
     gene            complement(18929..19330)
                     /locus_tag="PK264_02495"
     CDS             complement(18929..19330)
                     /locus_tag="PK264_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HRD74798.1"
                     /translation="MSLTSLVGTWALVRGTCTAADGTPRPAPYGPMGMGRVSFSADGR
                     MVAVVCDCRPELPAGTEREYNSYCGTYTFDGQRLVTKVFAFSDANRLSADQIRDIRVE
                     GRFIVMRPPPRKVPGGPDEQRELWWEKIADV"
     gene            19561..19899
                     /locus_tag="PK264_02500"
     CDS             19561..19899
                     /locus_tag="PK264_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HRD74799.1"
                     /translation="MGQCIVVVQFDLPKRTEELAIKGGSSTAPIYRGLAAKGLIRKDY
                     LNGDAGTGGVYLWSSRADAEAWFTDERMADLTKRFGVRPRLTWYDTHVTVDNVKGETR
                     VNGKALADAG"
     gene            complement(19902..20378)
                     /locus_tag="PK264_02505"
     CDS             complement(19902..20378)
                     /locus_tag="PK264_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD74800.1"
                     /translation="MSAKTEIRRADVPADVDAARALFREYAASLDVDLGYQGFEAELA
                     GLPGKYAPPKGALFIARSTGQAIGCVALRALDNPGICEMKRLYITPAGRGTGLGRALA
                     EAILAEARTLGYREMWLDALPSFKAAIALYKSAGFEEMAPYYATPVAGTVFLRRRL"
     gene            20511..22430
                     /locus_tag="PK264_02510"
     CDS             20511..22430
                     /locus_tag="PK264_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD74801.1"
                     /translation="MPLLFRALLTLLAAAIFGATPADARRVALVIGNGAYSHAERLAN
                     PTNDADAVAVALKRMKFDHVTLHRDLTADGMRRALGAFAAEATGADVAIVFYAGHGIE
                     VDGQNYLIPSDARLTRSAVVELETIPLTAVTGAIAGAQKLRLVILDACRNNPFRAKMA
                     AADTGPRRRSIGRGLARIEPGDNELIAFAAAGGTEADDGAGRHSPFTAALLKSLETPG
                     LEVRVLFGAVRDDVLAATSRGQTPHVYGTLGREAIYLVPPDAAGAKSSAEAEIARRVA
                     AEKARLEAEMAARLEAERKRIPSSPPATATVVPAPTPAPTAPSKGKLVTRAGALLHTF
                     DGHPDMVLSVAFAPGTIFSAGHDGTLKSWSLEKEALNWSVKAHDGATNAVAVSRSGFV
                     ATAGSDGLVKKWDVAKGKGARMNSFKIGTKSVTTAAIDRHSNWIAAGGANNSVMIWDA
                     NSERLIGTIDHHVNAITSLAFASDFPTLVIGSRDKTITVWRLNQVAWGSSWKNLERTI
                     NTGGDAVSAVAITPDGKLVISGSWDRLIKVWDRASGELVRTLEGHEGEVYSLAVTPDG
                     AHVLSASHDKTVRLWEIASGKLVRVHQAHAAYALAVAISQDGMIFASGGCDEFKDQKC
                     VKGLVKYWALVDRYE"
     gene            22530..23105
                     /locus_tag="PK264_02515"
     CDS             22530..23105
                     /locus_tag="PK264_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD74802.1"
                     /translation="MGTALFITGALVGAALIALLLATLARPALRIWPPPGPGTAQSYV
                     FWILFRSLNILCFAVAVMNREAYLGLPLWLRVVAFAVLVVSVATFIYAFKMLGRDNSY
                     GASEGLVTGGIYQWTRNPQNAMLIVVYGALAIAADNGPTYVLCLAMMLVYTLMVLLEE
                     PWLEFHYGDAYRRYCESVPRFFNWRRAFGRS"
     gene            23227..23823
                     /locus_tag="PK264_02520"
     CDS             23227..23823
                     /locus_tag="PK264_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRD74803.1"
                     /translation="MGSGEPRRLVGRRKGAEQPGASATTARTTGFPVGPIVSSAHLAA
                     GAAPGLSEVEFGLILASHAFERWMVRCMAAAGVGDLSPVEVLIMHTVRHRDRPKKLAD
                     VCLVLGIEDTHVVNYAIKKLTTAKLVASHRVGKEKVVAVTREGAEACARYHEIRERLL
                     IRAVKSTGLPDPMLSELGALLRALSGHYDQAARSATTL"
     gene            23882..24850
                     /locus_tag="PK264_02525"
     CDS             23882..24850
                     /locus_tag="PK264_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD74804.1"
                     /translation="MLKSVLTGLLGATLLAAPAMAQTKWDMPTPYPDGNFHTKNARAF
                     AEEVEKATGGKLKIQIHSAGSLFKHPEIKRNVRQALAPVGEVLISLHANESPFYGIDA
                     IPFLATSYAESAKLYAANRPFLEKKLAADGLVLLYSVPWPPQGIYAKKEIKSIDDLKG
                     LKFRTYNPGTARIAALVGAVPVQIEVPDLPTAFATGRVEVTITSGSTGVEARFWDFVS
                     HYHDTRAWLPRNVVFANKAAFDKLDADQKKALLAASAAAEARGFKASETETAERIAAL
                     KANKMNVVTPSPTLMDGLRKVGAQLAAEWEKAAGADGAALLAAYRK"
     gene            24985..25542
                     /locus_tag="PK264_02530"
     CDS             24985..25542
                     /locus_tag="PK264_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD74805.1"
                     /translation="MRAVLDGVYAATLRLSATCLVAIAVLVLAQVLGRLLDIALKLLG
                     LAPVGFIILSLSEIAGYLLAAASFLALAGSLKGGAHIRVTMLLGGVGERLRRVMELIV
                     FITALLFTAYMTYYVATLAFDSWRHNEISPGLVKVQLVWPQAAMAVGLGALVVALADE
                     IGILIREGRPSFRAAEDAVTLGKEG"
     gene            25547..26854
                     /locus_tag="PK264_02535"
     CDS             25547..26854
                     /locus_tag="PK264_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD74806.1"
                     /translation="MDLITLAFLLIFALFAVLATGMWIALALAAMGMLAMIVQSSAPA
                     GQVMASALWSSLNSWDLTALPMFIWMGEILYRTKLSSDMFEGLAPWLSRLPGRLLHIN
                     VAGCTIFAAVSGSSAATCATIGRIALPELMKRGYDERMAIGTLAGAGTLGLLIPPSII
                     MIVYAAATDQSVARLFIAGVIPGLVLAGLFSGYIIVWALFNRHRMPPADPPQSFASRM
                     AALRRLLPVVLLIVGVIGSIYGGLASATEAAVVGVALSLLLSYLMGTLSKASFSEALL
                     SATRTSCMICFILAGAAFLTTAMGFTGIPRALAEHVGSFNLPSWQLLTALTLFYIILG
                     CFLDGISMVVLTTSIIMPLIEKAGFDLIWFGIYVVVVVEMAQITPPVGFNLYVLQSMT
                     GRNLFQIGAYTLPLFALMVVMVILLAVFPGLVTWLPSTMLDTR"
     gene            26949..>27775
                     /locus_tag="PK264_02540"
     CDS             26949..>27775
                     /locus_tag="PK264_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD74807.1"
                     /translation="MADLHDHETLDAVWQRAVVEHRDRPFLCVPASASRDYATGGLEL
                     TYAAAAAEVDRLTEAYRAAGYGLGHRVALLLDNRPEHVLHRLALSRIGTSCVPINPDY
                     RPAEIAYLVEHSEPELVLALAAREEQVGAGLVMSSHKPHLRLLEAGAPPPPRSQARPG
                     EVTAATEGQILYTSGTTGRPKGCILSQGYEVASGAWYASLPGMARLGRARERIYNPLP
                     LYHVNAGVVSLIGAIVGGNCQILPDRFHPERWWPEVSATRASVIHYLGVVIQMLMKRA
                     "
     assembly_gap    27776..27791
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27792..28556
                     /locus_tag="PK264_02545"
     CDS             <27792..28556
                     /locus_tag="PK264_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD74808.1"
                     /translation="GVRFGFGAGVEPQLHAAFEARFGLPLIEVWGMTEMVRVLNDSVA
                     PRQIGTRAFGRPQPGVEIRVVDDHDREVPAGTPGEMTLRHSAATPRRGAFSGYLKNAE
                     ETERSWRGGWFHTGDVVRQDADGMLHFVERRKNIIRRSGENIAAAEVEATLLEHPGVM
                     QVAVLAAPDEVREEEVLACVVPRTGEGSEVLADELFQLCFERLAYFKAPGWIWFAPSL
                     PTTGTQKIQKHAIFPGLDDPRTAAGMRDFRARKRRG"
     gene            complement(28742..>29949)
                     /locus_tag="PK264_02550"
     CDS             complement(28742..>29949)
                     /locus_tag="PK264_02550"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRD74809.1"
                     /translation="IDTRRVAWRRVMDMNDRALREIVCSLGGVSNGFPREAGFDITVA
                     SEVMAILCLANDLKDLEKRLGDIIIGYRRDKTPVFCRDIKADGAMTVLLAQAMQPNLV
                     QTLENSPAFIHGGPFANIAHGCNSVVATKTALKLADYVVTEAGFGADLGAEKFFDIKC
                     RKAGLKPDVAVIVATVRAMKMNGGVKKEDLGKENVEAVKKGLANLGRHIENVKSFGVP
                     AVVGINHFVTDTEAEVAAVMDYVKSLGSECFLNKHWAEGGKGIAAMAKRVAEIADGGT
                     SKFAPLYPDDMPLAQKIEAIAKKIYRADGIVADKSIRDQLKTWEEMGYGKLPVCIAKT
                     QYSFTTDPNARGAPTGFTIPVREVRLSAGAGFIVAICGEIMTMPGLPKVPSSEHIRFN
                     EKGQIDGLF"
     assembly_gap    29950..29972
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29973..30447)
                     /locus_tag="PK264_02555"
     CDS             complement(<29973..30447)
                     /locus_tag="PK264_02555"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008530685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRD74810.1"
                     /translation="MASAAAPAKKYLSDIEIAREAKKMPIQDIGAKLGIPTAHLLPFG
                     HDKAKISEEFIKSLAAKKDGKLILVTAINPTPAGEGKTTTTVGLGDGLNHIGRRAAIC
                     LREPSLGPCFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAITSAHNLLSAMIDNHI"
     assembly_gap    30493..30502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30503..31409)
                     /locus_tag="PK264_02560"
     CDS             complement(<30503..31409)
                     /locus_tag="PK264_02560"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRD74811.1"
                     /translation="MRIVVHGQEAFGEAVLKKLLERGENVVAVCCAPDKAGRPEDPLK
                     LFAKAKGLPVHQPKSWKTPEALELMKSFKADVCMMAYVLLFVPQPVLDAPRFGTFQYH
                     PSLLPDHRGPSSINWPIAMGKTKTGLTIFWPDEGLDEGPILLQKTVDIGPDETLGDVY
                     FKKLFPIGVDAMIESLDLVKAGKAPKIDQGLKANPKLGSYESWFKKELAELDWSKPSA
                     EIYNTIRAANPAPGAWSTIAGKKVDIFDSARIKGPGIAGEVLSINGDGVTVGTGDDHR
                     IVVKRVRGADGKKIAAADWAKANDIL"
     gene            complement(31506..32783)
                     /gene="frc"
                     /locus_tag="PK264_02565"
     CDS             complement(31506..32783)
                     /gene="frc"
                     /locus_tag="PK264_02565"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HRD74812.1"
                     /translation="MTQALKGVRILDFTHVQSGPTCTQLLAWFGADVIKVERPGVGDI
                     TRGQLQDVPNVDSLYFTMLNHNKRSITLDTKHPKGKEVLERLMKVCDVLVENFAPGVL
                     DRMGYPWETIHKINPRLIVASVKGFGPGPYEDCKVYENVAQCTGGSASTTGFRDGPPL
                     VTGAQIGDSGTGLHLALGIVAALFQRTHSGLGQKVMCAMQDGVLNLARVKLRDQQRLS
                     HGPLTEYSQFGEGIPFGEATPRAGNDSGGGQPGRILKCKGWESDPNAYIYFITQAPVW
                     EAICDVIGEPGWKTHPDYAKPKARLPRLNEIFGRIEAWTMTKTKFEAMELLNKYDIPC
                     GPILSMKELAEDTSLRATGTVVEVDHPKRGKYLSVGNPIKMSASPTVVTRSPLLGEHT
                     DEVLATVLGYSADEMAAIKSSGATEAPKKAAAE"
     gene            complement(32814..33431)
                     /locus_tag="PK264_02570"
     CDS             complement(32814..33431)
                     /locus_tag="PK264_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD74813.1"
                     /translation="MSAIRELKRSTVEDYLAVDAASPIGHEFRDSVIYARGGATNDHG
                     RLAGNLFAATHGVLPPRCDVFMGDMRLKLRALSDDAYYYPDVLATCSDLDRARDSDVY
                     YPGIMVSCAASDRDRMFRREPILLIEVLSPSTGRLDRSEKRLKYLQIPTLQEFVLVAH
                     DRPMVEILRRRTSWLVEEHFPGDTLTLESVGISLPLAEIYRTNTF"
     gene            complement(33489..>33816)
                     /locus_tag="PK264_02575"
     CDS             complement(33489..>33816)
                     /locus_tag="PK264_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD74814.1"
                     /translation="ETICRYNLPVCVVVFNNNGIYRGTDVNKGGDDPAWTTFVKDSRY
                     ELMAKAFGGVGVYATSPDELSRAVNEAIDSGKPTLINAIIDEKAGTESGRIGNLNPQS
                     VVAKKA"
     assembly_gap    33817..33919
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33920..>34486)
                     /locus_tag="PK264_02580"
     CDS             complement(<33920..>34486)
                     /locus_tag="PK264_02580"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315864.1"
                     /note="catalyzes the formation of formyl-CoA from
                     oxalyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HRD74815.1"
                     /translation="VEKSGVPFLPMSMAKGLLPDTHPQSAGAARSMVLKDADVVMLIG
                     ARLNWLLSHGKGKTWGERGKQKFVHIDISPNEMDSNVQIDAPIVGDIASSVALMLSRL
                     GSDWPKPVAWMADIRGKVEGNVAKMAPRLQNNNVPMDYHGALGVLRRVMKERPETILV
                     NEGANTLDFARSIIDIYKPRKRIDVGTWG"
BASE COUNT         5681 a        11450 c        11179 g         5875 t
ORIGIN      
        1 agcggcacct cgaggccggc cgcgctcgag gcatctgcga gccacccacc gccgccgccc
       61 gaggccgtgc agatggcaac gcggcgcccc ttcggcggca gctgccgcca ggcaagaaat
      121 gcctgagcga ggtcgaccgc ctcgtcgagc tcgtgcgcct cgatcatgcc gaggctccgc
      181 gccatcgccg agaatgcggc gtacgagcct gcaagtgcgg cggtgtgcga cttcgccgca
      241 cgctggcccg catcggattt cccgatcttg acggcgatga tgggcttccc tgctgcctgg
      301 gccttccgac cggcgcgcac gaacgtggcg gcggtcttta catcctcgac gagcagcagg
      361 aagacgtctg cctcgtcgcg atcgatgaga tgctcgacga cgtcgaacac ctcgagctgc
      421 gcctcgtttc cggtcgtcac gacatggctg aacgcgagcc ccttcggccg cccccgatca
      481 tagaacgcga agcccatgcc gcctgattgc gcaacgacgg cgatgcggcc ccgcttgccc
      541 tcggggatga gcgggagcga cgtgtcggca accgccggac tgaaggtcac gcagagcttc
      601 gcgccgagcg tcgcgaagcc ctcggaattg ggcccgagca gggccatgcc gtacctgtcg
      661 gcgattgccc gcaaccgctg ctggctcgcc cggccggcct ccccggcctc ctccgcaaaa
      721 ccggacgaga tcaccaccgc cgcgccgacg cctgccttgc cgcagcgctc gagctcgtcg
      781 ggcacgttgg cggccgggat gacgagcacg gcgagttcgg gcgtctcggg gagtgccgcg
      841 acgctcgggt aggccttgag cccctgcacc tcggcaaggc tgcggctcac gggatgaacc
      901 gcgccggcga atgcgtgcat gcgcaacacc tcgagtgcgc ggccgcggat gccctgcccg
      961 atcggcgcgg caccgaccac cgcgacggag cgcggcgcga gaagcttcgt cagatcgggg
     1021 cggcgcgggc tcatggtgac cttgcttcgg ttgaatgtcg cgaagcgtac attcgcgccg
     1081 cggctcgaga aagcctatcg atcgagcgat cctgcacgac cggcatgcgc catgatcggg
     1141 tgccccacct tgcgctccac cgatcgatgg agaactatcg ggccttgcac cgcgggcagc
     1201 cagcgcgcgc cactcaaagc tccagggaac cgccgatgtc ccagccgacc gtgctctttc
     1261 gcaatgcgcg cctgctcgac ggccgcgccg attcgcccct cgatgatcac gacgtgctcg
     1321 tctcgggcgg catgatccgc gaggtctcgg ggaaacggct ctcggccgag ggcgcgatgg
     1381 tccacgatct cgccggccgg actctcatgc cgggcctcat cgactgccat gtgcacgtca
     1441 tcgccacgct cgccaatctc ggtgccaatg ccgagctgcc gaattcgctc gtggcgcttc
     1501 gctcggccga aatcatgcgc ggcatgctgc tgcgcggctt caccaccgtg cgcgatctcg
     1561 gcggcgcaga cctcggcctc cggatggcca tcgaggaagg cacgatcgtc ggaccgcgcc
     1621 tcgtcatctg cggcaaagcc ctctcgcaga ccggcgggca taccgactac cgcggccgct
     1681 accactcccg cagcgtcgac tactaccgcg accgcctcgg cacgctcggc cgcgtcgtcg
     1741 acggcgtcga cgaggtgcgc cgcgtctgcc gcgaggagat caagggcggc gccgacttca
     1801 tcaagctgat ggcgaacggc ggcgtctcct cgccgaccga cccgatcaag ttcttcggct
     1861 tctcgcgcac ggagatgctc gccgccgtcg aagaggctca gagcgcccaa acctatgtgg
     1921 cggcccacct ctacaccgac gaagctatac gccgcgctgc cgagtgcggg gtgatctcga
     1981 tcgagcacgg caatctcatc aagcccgaca cggcaaagta cctgaaggag aagggcgcct
     2041 acgtcgtgcc gaccaacgtt accttcgacg tgctcgccaa ggaaggcgcc gcgatgggcc
     2101 tgccgccaga gtccgtcgcc aagatcgagg acgtacgcag cgccggtctc tccgccctga
     2161 agctcctcgc cgaggccggc gtcatgatgg cgttcggaac agatctcctc ggcccgatgc
     2221 accaccacca gtcggacgag ttcctgatcc gcgcgcgcta cctgccggcg atcgaggtga
     2281 tccgctcggc gacagtcaat gcggccaagg tcgtcggtca ggaaggaaag ctcggcgtca
     2341 tcgcgccgga cgcacacgcc gacctcatcg tcatcgacgg cgatccgctc aaggacatga
     2401 acctgctcgt cggacagggc cggcacatgg ccgccatcat gaagggcggc agcttcgtga
     2461 agaacgcgct caagtgacgg caatggcgcg cccggcgggc gcgccacgtc gatatcgctt
     2521 ggcgagcgtc acggcgagct tctgccgggc cacatcttct tgtaccaggc atcatcgtca
     2581 ccaccaggga tggggggtgc cgacatcggc tggccggcct tcgccatctt ctcgcgaatc
     2641 atggcgatgt cccagcccgt gagatttgca agcgtcgccg cctctttctg gaagcgcgtc
     2701 tgcaggttgc gcttgtaggc ctcgcccgcc gagcacgtcg caggtcccgt gaacgggtgg
     2761 cggaggacat agcggccctg gaagttcgag ctgtccgccg tctcctggaa catgaggtcc
     2821 tcgggaaaat tgacggcgtc gtagcgcacg tggagccgcg tcacgaagac gttcgccgga
     2881 ccacccgggg gccgcatgcg ccgtggcatc acgcccggcc ccgacggcgc gccaggccca
     2941 tcgagcagcc agaaggcgcc gagctcgacg agcttgtcgt tcggcagcgg gtcggcggcg
     3001 cacgggtcgc accaggccat gttccaggcg tactcgaggt agacggcctt catgccgtcc
     3061 ttcttcacct gcgtctcgaa catcgccttg tagaaggcgc cgaactcgtt gcgcgtgaag
     3121 atcggcacgt ccatgtccga aggcagcttg agtgtgcggt agttcgtggt ctcgacgcgg
     3181 cccttctccg tcaggaagaa catgatcatg tcctgcgggc cgttggcgtt cacggtgccg
     3241 aggcggatcg gcagcatgaa cttggggctc tcgaaggcga ccgagatcgg gcgcaggtat
     3301 ttcgtgccga gcctgtcctt ctccttgagg ttcaccttgg cgacgaagaa cttcatgccc
     3361 tgcttgatgt agctcgcgag cacggggccg gcgccctgcg gcggcttgta gtcgttctcg
     3421 cggagccacg tctcgaggcc gccgctctcc ttggccgaga ggatcaggat atcgtactcg
     3481 ccgaccgtat actcggcctc gatcttgacg ccgagcgagc ggcggcggtc ttcgcgggcg
     3541 gcggcatccg cggagcgggc cgcggtcgcg ccgggcgcca tcgccatggg gcgagggatg
     3601 ttgcagggat cgggatcgaa gtactcgacg aggcgcggcg cggtgtaggc gtcgagatgg
     3661 tcgacgagcg cgttctcggt gatgttgatc tgccccttgg tgatcaccgt gggcacgggg
     3721 acgaccatgg cgaactcgct cgccgccccc tcgtagtcgc tcgccatggt gatgacggtg
     3781 cggtcaccct tgcgggccac caccaccttc gaggccttgt tgaagagctt ggtatcggcc
     3841 ttggcgacgt agaagccgca aaaggcttcc gccctgtcca tgggtgccgc aatggcgagc
     3901 cccacggccg cggtggcata gagcaatcgt ttcatgcgtc agtctccttg ctgttatccg
     3961 ttctcagatc gaggggcgcg accaggcata gcggtcggtg ctcgcgacgg gggcggcgcg
     4021 gtccatgagg acgacgagcg gtgagaggaa gaatagcgcg tacatgagcc cgttcggatt
     4081 gtagaggccg agctggatcc aggcgccgag gcaggcgacg agcacaccgt agaggatgcg
     4141 gtgcccgcgc cggtcgggcg tcgtcttcgg atcggagatc atgaagaagg cgaagaggag
     4201 gagcgcgccc gactgcatct gcttcatggg gatcgcccat ggatcgccga gccagagcgc
     4261 gcgcagaaag aggacagcca cgtatgtgcc gaggaaggcg agcgccacgt ccccgcgtcg
     4321 cgcccgcgaa agcaccatcg tcgcaaggca cgcgaacagg aatgcggccc acgtctggct
     4381 gccccactgc gagggcgaga tccaggcgag atcggtgaag agcagcagca ccgcgatcgc
     4441 gaaattcgtc gggttgaaga catgcttgcc gtcaacgcgc aggaagaact tcgagccgat
     4501 ggcgaggatc gcgccgagcg ccaataccca gagcgagcct gcccgcagga gcaggcagag
     4561 cgacagcccc gagatgagcg cgctcagggg atcgaaggcg cgcccgggtt gcatgatcca
     4621 gccgagaacg agctgcgtcg cgaggatggt cgcgaggatc acagagacgt tgataaggct
     4681 ctgatcgaaa ccgagccatc cgaggccgaa ggtcagcagt atgccgagcg aagcgatctg
     4741 taggtagcgc ggatcgaggg tgcggaccgt cgccagcagg cagcggctgc ccggcccggc
     4801 cgaagtcggg agtgagcgcg attgcaatcg agacgatgct ccaaacatgg gcgctccttc
     4861 ggccgggctc tgacacgctg taacggggca atctcacatc aatgccggtc tgacgcggca
     4921 gcgtggcgaa atcttggggg gcaaattcga ggtccgcgtc cgccgggcaa ggacgttgcg
     4981 ccgcagcgcc agattcctgt tcgccttgtt gtcggcaacc gacaggacta ggaaaaccgg
     5041 gcgtcaatcc gatcccggtc gagccccatg cgcttctccc tccgcctggc catcgttgct
     5101 ctcgccgcct tcgcgctcgg tggagccgtg gccttgctgg tgcttcccgg cgcgcgcgag
     5161 gccctcttca atccggtccg caccgtcgtc acgggtgcgg ccaccatcgg cgggccgttc
     5221 acactcaccg atcacacggg aaagcgcgtc accgaccagg atttccgcgg ccgctatatg
     5281 ctggtcttct tcggcttcat cttctgcccc gacatctgcc ccggcagcct gcaggtcatg
     5341 accgaggcca tggagcgcat cgggccgaaa caggctggga agatcacgcc actcttcatc
     5401 tcggtcgatc acgagcgcga cacgccggcc gagctcaagg agtacgtgaa gagcttccac
     5461 ccacgtctcg tcggcctcac cggctcggcg accgacatcg ccgccgccgc caaggcctac
     5521 cgcgccatct atcgccgtgt cgcggaccag aagtcgaagg ccggctacac gatcgaccac
     5581 acgacgttct tctacctgat gggcccggac gggaaattca tcacccactt cacgcacggc
     5641 gtgctgccgg acgtgcttgc taagcggctc gcggcgctgc cctgagcaaa tttgcgctgc
     5701 atcacgtcga agcagctcga gccgttactc cgtccggtga cctcagacct tgccttcgcg
     5761 aggacgacga gtcgggattt cgggcgaacc cgtttcggcc ggcgtgggtc gtcgcaaagc
     5821 gtacagggtt tcctctggcc gaagtgtagg gcagatcgga catcgtactg gataccagac
     5881 acgcggtgca gcagcagccg agcagggcag ctctcgccgg cgcatacgct ctggcaactc
     5941 ccgccagcgc catacgctgc aacatggccg aaacacgccc acggcctcat gacccgcccg
     6001 ggttgtcgag atcgcctatc tgacctctaa agagcgcacg gcttcctgcg gcgtaacgcg
     6061 agagcggcga acaagcaacg ctcgcccctt attcttgtcc gccaagtaaa cctttgcggc
     6121 actgctatgg cattaaccgt accgggactg tatttaatct gagacggcac ttggcatgct
     6181 aggggcagat tcggtgccca cgacttggcg tcggcacgag caaccttgag gtgtgcgcgc
     6241 atgatttcag ttggcagggg aaaagctcgc cgctaaagag gccacgcgac gtgcgggacg
     6301 cggcgtcccg cttttggggt caacaatcaa agagttccaa cgacgtgtcg aaggcgcgta
     6361 tgaagtgtca tgtgctcgtc gacttcgacg gcacgatcgc aacggtggat acgaccgacc
     6421 tgctgctcga gcagttcgca gatccaagct ggcaggagat cgaggccgac tggaaatccg
     6481 gtcgcatcgg ctcgcgtgaa tgcatgatcc gccagatcga tctcgtgcgt gcctctccga
     6541 cggagatgga cgcgttcgtc gcgggcatcg agatcgatcc tggcgtgcct gagttcatcc
     6601 ggctttgcac ctcgctgggt caccgcgtca gcgtggtgtc ggacggcctc gaccgcactg
     6661 tcggcgccgt gctgcgccgg cacggcatcg atgtccccca tttcgcgaac catctcgagc
     6721 acgtgggcga gcggcgctgg cggctgacgt tcccctgggc gcgcgacgac tgcaaaccgc
     6781 tgtcgggtaa ctgcaagtgc cagttcgcga ccagcacgac gcggggcctg cgcgacatcc
     6841 gcgtcgtcgt cggcgacggt cgctccgatt tctgcgtcgc tgagggcgcc gacctcgtgc
     6901 tcgcgaagtc ctcgctcacc cgccattgcg ttgcgaacga cctgccgcac ttcgccttca
     6961 ccacattcgc cgaagcgacc gacctcttcg cgcgctggct cgatcagcgc gtcgtcgcca
     7021 acggccgccc cttcgagcag taaaggagta gagaatggac gcccccnnnn nnnnnnnnnn
     7081 nnnnnnnncc cgccatgacg gacgaggagc tgctcgccct cgaggcgcgc tactgctcgc
     7141 acggcgatac cgtgcactac acgaacccgc cgaagatctt cacgaactgc gaaggctcgt
     7201 acatgtacga cagcgcaggg cgcgagttcc tcgatcttca gatgtggtac tcggctgtca
     7261 acttcggcta caagaacgag cgcatcgcca atgcggtccg ccgccagctc gacacgctgc
     7321 cgcaggtggc ttcgcaatac ctccaccgcg agaagatcga gctcgcgacg ctgattgccc
     7381 aggacgccga gcgcaaattc ggcgagaagg gccgcgtcca cttcaacgtc ggcggcagcc
     7441 aggcggtcga ggacagcctg aagctcattc gcaacttcaa gggcggcaag cagctcatga
     7501 tcgccttcga gggcggttat catggccgca cgctcggcgc ctcagcgatc acctcgtcct
     7561 accgctatcg ccgccgctac ggccatttca gcgatcgcgc catgttcatt cccttcccct
     7621 accatttccg gggaccgaag ggcatgacca aggaggagta cggcgagaag tgcgtgaagg
     7681 acttcgcgcg cctcttcgag agcgagtact acggcctctg ggaccccaaa gcgaacgagt
     7741 gcgagttcgc cgcgctttac atggagacga tccagggcac gggcggctac gtcgttccgc
     7801 cgatgaactt cttcaaggac ctgaagaagg tgctcgacca gtacggcatc ctgctggtgg
     7861 tcgacgagat ccagatgggc gtctaccgga cgggtaagct ctggtcgatc gagcacttcg
     7921 gcgtaacgcc ggacgtcatc gtgttcggca aggcgatcac caacggcctc aacccgctct
     7981 cgggcatctg ggccaaggaa gcgctcatca acccgaagac cttccccgta ggctccacgc
     8041 actcgacctt caacgcgaac cccatgggga cgggcgtcgc gctcgaggtc atgcgcatgt
     8101 tcgccgagcg cgactacgag gcgatggtga tggagaaggg ccgccgcttc ctcgcgggcc
     8161 tcaaggacct ccagaagcgg cacaagatca tcggcgaggt tgatggtctc ggcatggcgc
     8221 tgcgcgcgga gatttgcgag ccgcacgaca gcttcacgcc ggcgaagcgc atcgtcgaca
     8281 tgatggtgga cgagtcgctc aagggcgacc tcgaggtgaa cgggcgcaag tacggcgtcg
     8341 tcctcgacat cggcggctac tacaagaatg tcataacgct ggcgccgagc ctcgagatgt
     8401 cgcacgccga gatcgacctc gcgttgacgc tcctcgacaa gctgatgacg cgcgtgacaa
     8461 aggcgctgtg atcagacgca tctggatcga gctcggtccg gctggtatga gccgtcgtcg
     8521 acggggcggg ccgaagcctg ccccgtttcg ctatccggac aacgctcccg gcaggtggca
     8581 tgacccccta tgcgctaatg ttcctcgccc tcgtgctgat cggcacgacc acggggcagc
     8641 ttctcttcaa ggccgcgtca gtccgcgcgg agggcgatgg ggtcggcgag cactggcggt
     8701 cgctgctcac cgacaagctc atctggatcg ccatcaccat ctacatccta gagttcttca
     8761 tctggatggc gttcctgtcg ctcgtgcccc tctggcaggg tgtgatggtc gcctgcgccg
     8821 acatcgtgct cgtgatggtc ggcggacgca tcttcttcgg cgaggagatc acaggcgcgc
     8881 gcgtcctcgc gatcagcctc atcgcggccg gcgtgctgct cgtcgggctc ggaggcggca
     8941 aatgatccgc acgaccatca tcgtcggctt catcctgctc atctcgctcg atacctacct
     9001 gcagatcgca ttcaagctcg cgagcgaggc gacgctgccg atgaccttcg acatcgagtg
     9061 gttccgccgc gtcatccgca gcccctggac gcattcgatc ctagtcggct acgtgttcgc
     9121 cttcctcatc tacatgacga tcctgaagca tgcgccagtc ggccctgcgt ttgcggcctc
     9181 gcatgccgag atcgtcatcg tgaccatcat cggcgtcctc tatttcggcg agagcatcac
     9241 cttcgtgcag gccgctggct gcgcgctgat cgttgccggt atcggcgtgc tggcggcaac
     9301 ggagagaggg tagcgcgatc ggccgggcgt caggccgcac gccgcgcgac aagctcgatc
     9361 atgcgcgcgc gcgctgccgg cagctcctga ccggccggcg catagacccg cgccctcagg
     9421 aagtgccccg tgagcgcaaa acctgccgcg atatcctcct tcgaggctgc cccaccgctg
     9481 ccgatcagga acttcggcag cgcgaggaga cggtcgcgat aggggaggcc cgccatgtgc
     9541 gagaccgcgc ggcccgagcg cggcgacacc cactcgagcg cctcggtcgc tccggttgcc
     9601 gcgcagctcg tgagatcgag gccgaagccg agctcgccga gcagagccag ctcgaagcgg
     9661 accatcagcg ccggccagat gtcggcgtcg tcgaggtagc cgagcacgaa atgggcgacc
     9721 tcgaagagcg agggatgggg ctcacgctcc ggcagcagat ggcagatcgc tgcaagcgac
     9781 atgagcccgg cgagcgccgc gggatcgttc ataaactccg cggcaaagcc tcggcgcagc
     9841 tcgaccgagg cgtgcccgag ctggtcctcg aggcgcgcct tccaggtcgc atcaacgtgg
     9901 tttccggcct gcagcaccgg acgcaacttg cgcgaccgcc caccgtgcac gagcacgacg
     9961 cagcggcccc gcgcgcgcgt gaaaagctcg gcgatcgcgg ccgtctcgcc atgtggccgt
    10021 gtcgagagga tcagggcctc gtccgtccat tccatgtggc gccggtcgac cgctggagtt
    10081 gggatgacgt acatcagggc agaatcaagc gctgcccgcc tcacccccga acatggtgcc
    10141 ggcaccgtgc tcggtgaaga gttcgagcat tacagcgtgc gcgaccttgc cgttgaggat
    10201 caccaccgcc tcgacgcccg cctcgaccac ctcgatgcag ccctcgacct tcgggatcat
    10261 gccacccgtg atcgtgccgt tgcggatgag ggcgcgcgct tctgcgaccg agagccgctc
    10321 gaggagcttc ttgtccttgt cgagcacgcc ttcgacgtcg gtgaggagga ggaggcgctt
    10381 cgccttgatc gcggcggcga gcgcgctcgc gaacgtgtcg gcattgatgt tgagcgtctc
    10441 gcccgaggcc gacatgccga tcggcgcgac gacgggaatg aggtccgagc gcgtcattac
    10501 gtcgatgatc tcggaattga ccgccatcgg atcgccgacg aagccgatat cgacgggctt
    10561 gacgatgccc gtctcgggat cggggagctc ggtcaccttc ctggcgatca tcaggttggc
    10621 atccttcccc gagatgccga ccgccttgcc gccctgcttg ttgatcgccg cgacgatctc
    10681 cttattgatc tgcccggcga gcaccatctc gacaacctcg acggttgcct tgtcggtgac
    10741 gcgcaacccg tagacgaaat cggacttgag ctggagccgc ttgagcatgt tggcgatctg
    10801 cggcccgccg ccatgcacga cgacagggtt cacgcccgcc tgcttgagca tcacgatgtc
    10861 cttggcgaag gcttccgcca gcgccggatc gcccatcgca tggccgccgt acttcacgac
    10921 cacggtctgg cggtcgtagc gttgcatatg gggaagcgcc tcggcgagca cgcgggcctt
    10981 gccggcggcc gagtcggaga cgcccttcgg ggcgggcgcg gcctcgtggc ccgattttgc
    11041 catgctggtg ctccttcaac tggtgccgcg gccggagcgg tcgagaccgc gcgatctaga
    11101 ccggcacgac gcctcgcgac aatggaatag tgcgtcgcaa ggtgagcgcg ccatgnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnntcg cagcgccact atgctggtgt aggcggcgca cgacaggctt cgacgcggtg
    11281 tcgggcgtcg agatgacaga cgtcgaggag gaggcaaccc aggatggcgg tcgccgagga
    11341 catcggccag gcgccggcgg cgagagcgct cgcgatcacc ttgtcgcgag cgacccacta
    11401 tgcctatgcg cagggccacc gctacatcac catcgagcat gcactccttg ccctcaccga
    11461 ggacgccgaa gcctgccgcc tcgtcatgga ggccgggggc gacgtccagc gcctccgcga
    11521 tgacgtggca gcttacctcg ggcgcctccc ggatcgcctc gccccgggcg agcgcggccc
    11581 gatgatccat cccgagctca cgcagatcat cgactacgcg agcgccgccg cgcgccgcag
    11641 cccgcggccg caggtgagcg gagcagtcct gctcgcggcc atcatcggcg agggccgctc
    11701 ggtcgcggca agcatcctca cctcgcaggg gttgacgttt cagagtgcca tcgatgcgct
    11761 gacgcgctcc accgtcgctc cgctcgaccc accagcctat gcccaacccg atgcgaccgg
    11821 tgacctcgag ctgccaccct ggccggcatc gcccactccc tccgccaatc ccgcggatgt
    11881 ccccgcgccg cccccttacc cagatgcctt accgggattg ggcagtgggg ccccgctgcc
    11941 gaccgagccg ctgttgccat tcccgagcgc cacgcctgcg ccgagcggcc cgatggagca
    12001 gctcctagcc cgcgcccgtg aacgcgtgaa cgcaggccgg cccgttgcct cagagccacc
    12061 gagaggccaa tcgcacctct cgcggcagcc ccacctccac ctacattgcc ggacccgccg
    12121 acggagcgcg cgctacccac tctggcgccg actgccgagc ccacgcacct cggcaccttg
    12181 ccaaaactgg agctgccgcc actcttcgag gagcgatcgg cccccaccct tgtgacgcac
    12241 gcgccgccgg gccttccggc gcctccccag cggacgggcc acatgccgac gccgcgcacc
    12301 gatcatgccc cccagttgcc gccagccctg ccgccggctg acctgcttct cccccatggg
    12361 catccgccat acgttgctcc gcagccctta ccttcgccac cgcccgcgcc ggcctacggc
    12421 ttcgaccctc cgttcgatgc tgcgccgccg atgacagctc cggcaccctg gccggatcct
    12481 gcgggtctgc cgccgcccgg ctatgccggc acggtcccgc cgacgccggc cgatgacaat
    12541 ttcgccggcg ccccgtatcc ggatgagacg atcgatcagc tgcgcgcccc taccggcacc
    12601 gagcccggcg gcgtgagagc cccgctcgga atcgccccgg ggcaactcgt cgagaacatt
    12661 ccgcgcgcca tgcggcttgc gcttgcgcac agcgtcgagg tcaggatcgc gcgcgaggtc
    12721 gtcggcaatg tcgcgtcagg aatgcctggg accgtgacgg cggcactgat catggtgacg
    12781 cgctcgatgt cggtgcggct gcgctcgccc gatggcgcct tccacatcga gacgatctcg
    12841 cccgaaacgc agtggatcga caaccgtttg ggcctcatgg tcgatgacta tgcgagctgg
    12901 cggtggacgg tgacgccgcg gcggcgcggt acggccgagc tccagctcgt cgtctccgcc
    12961 cgcaccgtcg gcgacgacgg cgtcgttgcc gagacggcgc tacccgacca ggtcgtggcc
    13021 gtctccgtca aggtcaactg gcccctcacg ctgcggcgct ggggcgggtg ggccggcgcc
    13081 atgctcgccg gcggcatcat cggcaagctt ggtgagggcg gcctcgcagc cggcgtttcg
    13141 gccctacgac aagccatggg actttgaggc cggcgtgtgg aggaatagga ggtgccaatc
    13201 ggaggcgctt cccaatatgc ccgtcgggtg gtatctgcgn nnnnnnnnnn nnnnnnnnnn
    13261 nnnnnnnnnn nnnnnnccgg cccgcacgcc tcacggacga ggcgcttgcg ggccgcgcgc
    13321 gttccgggct cgaggccgaa accgcgagtt cctcgatgat gcagcaggca cgcgatccag
    13381 gaaagggctg acccatggcg catcgccccg ttgagatcag agacgatatc ggcctcacct
    13441 tcgatgatgt gctgctggtg ccgcagggct cggaggtgct cccgggcgag gccgacgtct
    13501 cgacccgcat caccaaggag atcagcctca atatcccgat actctcgtcc gccatggaca
    13561 cagtgacgga ggcgcctctc gcgatcgcca tggcacaggc gggcggcatc ggcgtcatcc
    13621 atcggaacct gtcgcccgag acccaggccg accacgtcgc ccgcgtgaag aagttcgaga
    13681 gcggcatggt cgtcaatccc gttacgatcc acccgtcggc aacgctcgcc gaggcgctgg
    13741 agctgatggc ccgccaccgc atttccggca ttcccgtggt cgagcccggt cgcaccggca
    13801 agctcgtcgg catcctcacc catcgcgacg tgcgctttgc ctcgaatacg cgccagctcg
    13861 tctcagagct catgacgcgg gaaaagctca tcacggtccg cgagggcgtc gtccgcgacg
    13921 aggcaaaacg cctcctccac cagcaccgca tcgagaagct gatcgtcgtc gatgatgcgt
    13981 accgctgcat cggcctcatc acggtcaagg atatggagaa ggcccagcgc caccccagcg
    14041 cctgcaagga cgagcagggc cggctccgcg tcgcggcggc aaccagtgtc ggtgacgacg
    14101 gcttcgcgcg caccgagcgg ctgatcgacg ccggctgcga cgtgatcgta atcgataccg
    14161 cgcacggcca ctcgaagagt gtgctcgagc aggtgacccg catcaagcgg ctttcgaacc
    14221 gcgtgcaggt catcgccggc aacgtcgcga cggcggacgg caccaaggcg ctgatcgatg
    14281 ccggcgcgga tgccgtcaag gtcggcatcg ggccgggctc gatctgcaca acgcggatcg
    14341 ttgccggcgt cggcgtaccg caactcaccg ccgtcatgga cgccgtcgag gaagccgcca
    14401 agcgcgacat cggcgtcatc gccgacggcg gcatccgcta ttcgggcgac attgccaagg
    14461 cactggcggc gggtgcgtcg gccgtgatgg tcggctcgct gctggcgggg actgacgagg
    14521 cgccaggcga gacctatctc taccagggca ggacctacaa ggcctaccgc ggcatgggct
    14581 cggtcggcgc catggcgcgg ggctcggccg atcgctattt ccagcaggac gtcaaggatc
    14641 agctgaagct ggtgcccgag ggcatcgagg gccaggtgcc ctataaaggc ccggccggtg
    14701 ccgtcgtcca ccagctggtc ggcggcatca aggccggcat gggctatgtc ggggcgcgga
    14761 ccttgactga actcaatcag cgcgcgcgct tcgttcgcat atcaccggcg gccatgcgcg
    14821 agagccatgc ccatggcgta ctcatcacgc gcgaggcgcc taactatccg ggcggctgag
    14881 ttcagtcggt cagagcgggc cgcttgagct tccgcttggc gcgcggcgtc tcgggcggtg
    14941 tcggcactat gctggtccag cgctcgatgc gcacgccggg aaaaatgacg atctcggcgc
    15001 tgccgcccct gcgtggcaca atagggccgg gccgcgacgg ccgctctagc accgggaact
    15061 cgatgacgtc tgccatggat ccctcctatc caggcgaggc cgccgatccc gcggcagccc
    15121 ttcatgcaat catcatgacg gctgcttcgg ttaaccaaag attaccgtcc gaggcgatct
    15181 gcatgcagtg cttcgcgcag tttcgccaag tggtaaattt gcaccaaata ttgccatccg
    15241 tgggtgaagt gccgagaatc cgtcatcaat cggtcggggg gaattgttgc agatccgtga
    15301 ctcgctgtgc cgcgatggcg ccggatcacc tcaccgcggg ccgacgccgc tcgccccaca
    15361 tgttgatcag gttggccgcg acgacgatca tcgcccccgc catcacccag gggttcagcg
    15421 gctcctcgta gagcatcacg ccgacgacgg tgatgatcgg caggcgcatg aagtccattg
    15481 gcgcaacgat gatcgcatcc gccacgctga aggcccgcgc catcgaatag tgggcgccga
    15541 gtccgaagac cgcaacggcc gccacgaacg cgtaggtggt cgcatcgtgg aaacggagat
    15601 aggggatcgc cggaagcgca ctgatcaggg cgnnnnnnnn nnnnnnnnnn agagcaggat
    15661 cgtcgcggcg ctgtcggtac gggtgagccg acgcgtggcg atcatggaca gggcgaagcc
    15721 gagcgccgaa atacaggcga agagcgcgcc acggccgaaa ctcgcctcgc cgggattggc
    15781 gacgacaatg gcaccgacaa agccgatcat cgccgcggcg agacgtgtgg ccgtcagccg
    15841 ctccccgatc agcagtggcg cgaggactgc gacccacagc ggcgcagtaa actccagtgc
    15901 gaagagctgg gcgagtggga tcaatgcgag cgcataaagc caggtgaact gtgcgatcca
    15961 gtgcacaccg ccgcgtgcga tctggagaag cggctgcccg gtgcgcatcg cgaggaccag
    16021 ctgaccggtt gcggcgagga tcgggatcag cacggcgagt gcaatcacgc tgcgcaggaa
    16081 gatcagctcg atggtcagca tgccgcgccg ctgcgcctca cggccggcta tggcgatgac
    16141 ggtgaagcag cagagtgcca ccgtcatcca gagcagcgcc acgaccacgc cagatcggcg
    16201 cggcgccggc accactgcat tcgtttcgtc ctctccggcg tcggtcgtgg ccatggctca
    16261 gcgtgtcgcg gcgtgcaggc tcattccgcg gccatgaccc gcgacggcgg cacgaacccc
    16321 tttggcaggc ctgcgcgggg gacgccgccg taaaagcgct cgcccggtag cgccttctcg
    16381 gcgatggcgc gcgcctcgcg ataggcctcg agattgacgc cggtctttag gccctccttc
    16441 tcgagcagga agaccaggtc ctccatgacg atgttacccg tggcaccggg cgcaaagggg
    16501 cagccgccga ggccgccggt cgaggcgtcg aaggcgccga cgccggcctc gagcgccgca
    16561 tagacgttgg cgagtccgag cccgcgcgta tcgtggaagt gcgcgtagac gggcttcccc
    16621 gttgccaacg tcactgtgtg gtggaagagg ttctcgacct cgctcgggcc ggcatagccg
    16681 accgtatcgg caagcaggat ctcgtctgcc ccgagcttca ggaagcgctc gacgaaggtg
    16741 agcacgcggt cgggatcgac cttgccctcg agcgtgcagc cgaacgacgt cgcgaggcag
    16801 ccctggattt tcaccgattt ccactcgggc ctcgagcccc gcagctcgac gatctgccgg
    16861 aactcgtcga aggactggtc cgaggtcttg cgcacgttcg cgaggttgtg agtctcggag
    16921 accgacatca cgaagttgag cttcttgagg cccgtcgcca gcgcgaactc ggcgcccttg
    16981 cgattgggcg ccagcgcgca catcagcgat cccggaaagg cgagcgagcc gcgcgccacg
    17041 tccgcggcat ccgcgaactg ggcgatgacc ttcgagggca cgaaattcga gacctcgatc
    17101 tccggcacgc ctgcggccga cgcggccttg atccaggcga gcttgatctc cgtcggcagc
    17161 atggtcttga ccagctgcag tccgtcgcgc gtgccgactt ccctcacgat gatgtcacgc
    17221 tgcatggggc gtctcctgac gtcagacgaa gatggccgat cggccgttgg aagtcacgag
    17281 gggcgatacg tttcgcggcg ctcggcgagg atctggccgc ggatcgcgaa cacgatttcg
    17341 ccgtgctggt tgcgggcctg gctgtccgtc tggatgaggc cgcggtcggg ccgcgacttc
    17401 aagtcgattt tcccagccgt gcggccgcgg aacgtcaacg tatcgccggg atagacgggc
    17461 ttcagccagc gcagattgcg aaatcccggc gaggggccgt agtgcgcgac cttcaggccc
    17521 ttgtcgcgca tctcgccctc gatcttctgg cggaaggcga cgaactggcg gatgaagagc
    17581 gccgccgtat gccagcccga ggcgctgagg cggccgaagt aggttttcgc cgccgcggcg
    17641 tcgtcgaggt gaaaaggctg cggatcgaac tcacgggcga aggcaatcac ttcctcgcgc
    17701 gtgagcgtct gcgagccgag atcgaaggtc tcgccgatga cgcgatcctc gaagtagttg
    17761 ctcgtccgcg agggctcggg tcgcggcggg ccatcccaga ggctctcgag cctcgccgat
    17821 gtgccgctgc ccgcgccttc cggggcgcgc tcgacgggct tgggatcacg caccttcacg
    17881 agctggttcg agaaccaggt ctgcaccaca tcgcccgagc cgttcagcat ctccatggtg
    17941 atcttggcga gcccaacctc gggccgcgag ccgagcgcgc gcttctcggt gcaggtgtag
    18001 cggccggtca gcatgtctcc gggccgtacc ggcttcgacc acttcacctc gtcgatgccc
    18061 ggagagccga gcgaggtcca gcggttgagg aggccgtcgg cgaggagccg catttgcagc
    18121 gcgcaggaat gaaagcccga ggcacagagc ccgccgacga tcgagcgctt cgccgcctcc
    18181 tcgtcgagat ggatgggctg gggatcaaag gcccgggcaa aagcgacgat atcctctttg
    18241 gaaaccgaga gcttgccgaa cggaagtgtc cggccgacct cgatgtcctc gagatgaacc
    18301 cgctcgctct ctgatacgat ctcgcgatcc attgccgtca tgaacgcctc actccgcacc
    18361 gcagccttgg tagcggccgc aaatcccgcc ggcaagcaca tggggtccca caagagcaca
    18421 tgccgcgctg gcggttccgg aatagcaacg gcccggacac gcgcagtgcc cgggccggaa
    18481 tgtgtcgatc gagccccgac ttgacgtcag acgccgaagc cttcgaactt cttcttgaag
    18541 cgcgagacgc ggccgccgcg atcgaggatc tggttgccgc cgccggtcca ggccgggtgc
    18601 gtcttcggat cgatgtcgag gttcagcgtg tcgccttcct tgccgtaggt cgagtaggtg
    18661 acgaactcgg tcccgtcggt catgacgacc ttgatctggt ggtagtccgg gtggaagtcc
    18721 tcggtcttct tcatcggctg tcctcggcct cgggctcaat gcaactggcg cccggacagg
    18781 ctcggcgagc tgtcggcggc acctcagtgg ttggatctcg tccggtctat acgggaagcg
    18841 cagacctgaa acaaggcgct tttgctggcg tgccctgtcg cagaccgccc tgccgacccg
    18901 gctccaggtt gcggctgcag cctgcctctc agacgtccgc gatcttctcc caccagagct
    18961 cgcgctgctc gtcaggcccg cccggcacct tgcgtggcgg tggcctcatg acgatgaagc
    19021 ggccctcgac gcggatgtcg cgaatctgat cggcgctcag cctgttggca tccgagaagg
    19081 cgaacacttt ggttacgagg cgctgaccgt cgaatgtgta ggtgccgcag taggagttgt
    19141 actcgcgctc agtgcccgcc ggcagttcgg gccggcaatc acacacgacc gccaccatgc
    19201 ggccgtcggc cgagaaagac acacgtccca tgcccatcgg cccatagggc gcgggccgcg
    19261 gggttccatc ggccgccgtg caagtgccgc gcacgagcgc ccaggttccg acgagcgacg
    19321 tcaaagacat gtcagatact catatttgag gcgggcgcat cgcccgtcac cgactgttgc
    19381 ctgctgaagc ccgctcgcgc aaggctctgc ctcgtcgcaa gcaacgcggt ttccaaaggc
    19441 ctgaggcctt tggcggggtg cggggcagcg ccccgcgcct tccctcgcgg cactcctttc
    19501 aatcacatgg ccggcgcgct aaactcgcat cttgatgtca cgcattcgga ggacaccgat
    19561 atgggccagt gcatcgtcgt cgtgcagttc gatctgccga agcgcacgga agagcttgcc
    19621 atcaaaggcg gttcgtcaac agcccccatc tatcgcggcc tcgccgccaa ggggcttatc
    19681 cgcaaggact atctgaacgg cgatgccggc acgggcggcg tctatctctg gagcagccgc
    19741 gccgatgccg aggcctggtt caccgacgag cgcatggccg acctcaccaa gcgcttcggg
    19801 gtacgcccgc gccttacctg gtacgacacg cacgtcaccg tcgacaatgt gaagggcgag
    19861 acgcgcgtca acggcaaggc gctcgccgac gccggctgat ctcagaggcg gcggcgcaag
    19921 aacacggtgc cggcaacggg cgtcgcgtag taaggcgcca tctcctcgaa gccggcgctc
    19981 ttgtagagcg cgatcgccgc cttgaatgag ggcagcgcat cgagccacat ctcgcggtag
    20041 ccgagggtcc gcgcctcggc cagaatggcc tcggcgagcg cgcggccgag cccggtgccg
    20101 cgacctgcgg gcgtgatgta gagccgcttc atctcgcaga tgcctggatt gtccaaagca
    20161 cgcagtgcca cgcagccgat ggcttggccg gtcgagcgcg cgatgaacag cgcgccctta
    20221 ggcggcgcat acttgcctgg aagccccgcc agctctgcct cgaaaccctg atagccgagg
    20281 tcgacgtcga gcgaggccgc atattcacga aacagcgcgc gagcggcatc gacgtcggcg
    20341 gggacgtcgg cacgcctgat ctcggtcttg gctgacatga ggtcgttcgc actcggcttc
    20401 gatcggcgct gaagttgggg cgcttgtttc tctcccgtct cggcctctct ggcaaccagc
    20461 ggattcacgc gttaggctca cgccaatacg cattcgagcg agccttgccc atgcccctcc
    20521 tgtttcgtgc gctgttgacg ctgctcgctg ccgccatctt tggcgcaact ccggcagacg
    20581 cccgccgcgt cgcgctcgtc ataggcaatg gtgcctacag ccatgccgag cgactggcaa
    20641 acccaaccaa cgacgccgac gccgtcgccg ttgccttgaa gcgcatgaag ttcgaccacg
    20701 tgacgctgca ccgcgatctg accgccgacg gcatgcggcg cgcgctcggc gcatttgcag
    20761 ccgaggcaac aggtgcagac gtcgccatcg tcttctatgc cgggcacggc atcgaggtgg
    20821 acgggcagaa ttacctcatc ccgagcgacg cacgcctcac ccgctcggcc gtcgtcgaac
    20881 tcgagacgat cccgctgacg gcggtcaccg gtgcgattgc cggcgcgcag aagctccgcc
    20941 tggtcatcct ggacgcctgc cgcaacaatc cgttccgcgc caagatggcg gcggccgata
    21001 cgggtccgcg gcggcgcagc atcggccgtg gcctcgcccg cattgagccc ggcgacaacg
    21061 agctgatcgc cttcgccgca gcaggcggca cggaagccga cgatggcgcg ggccggcaca
    21121 gtccgttcac ggcagcactt ctcaagagcc tggagacgcc cggcctcgag gtccgcgtgc
    21181 tgttcggcgc ggtgcgcgac gacgtattgg ccgcgaccag ccgcgggcag acgccgcacg
    21241 tctacggcac tctcggccgc gaggcgatat acctcgtgcc gccggacgca gccggcgcga
    21301 agtcgtcagc ggaagccgag atcgcgcgcc gtgtcgcggc cgagaaggcg cgcctcgaag
    21361 cggagatggc cgcccggctc gaggcagaac ggaagcgcat tccgtcgtcg ccgccggcga
    21421 ccgcgaccgt tgtgccggcc cccactccgg ctccgacggc accctccaag ggcaagctcg
    21481 tgacgcgcgc aggcgccctc ctgcacacct tcgatggtca cccggacatg gtgctctccg
    21541 tcgccttcgc gcccggcacg atcttcagcg caggtcatga cggcacgttg aagtcgtgga
    21601 gcctggagaa ggaggcgctc aactggtcgg tgaaggcgca cgatggcgcg acgaatgcgg
    21661 tggcggtgtc gcgcagcggc ttcgtggcga cggccggcag cgacggattg gtcaaaaagt
    21721 gggacgtggc caaaggcaaa ggtgcgcgga tgaactcgtt caagattgga acgaagtccg
    21781 tgacaaccgc cgcaattgat cgccattcca actggatagc cgcgggcggc gccaacaact
    21841 cagtcatgat ctgggacgca aactcggagc gacttatcgg caccatcgat catcacgtca
    21901 atgcgatcac atcacttgcc tttgcctccg acttcccgac gctcgtcatc ggcagccgag
    21961 acaaaacgat taccgtctgg cgattgaatc aggtcgcgtg gggctcatcg tggaaaaatc
    22021 tcgagcggac gatcaacaca ggtggtgacg ctgtttcggc agtcgcgata actccggatg
    22081 gcaagctcgt catcagcggc agctgggacc ggctgatcaa ggtctgggat cgcgcctcgg
    22141 gcgaactcgt ccgcacgctc gaaggccacg aaggcgaggt ttactcactc gccgtgacgc
    22201 cggacggagc gcatgtcctc agcgccagcc acgacaagac cgtgcgcctc tgggagatcg
    22261 cgagcggcaa gctcgtccgt gtgcaccagg cgcacgcggc atacgcgctc gccgtcgcca
    22321 tttcccagga tgggatgatc ttcgcgagcg gcggctgcga cgagttcaag gaccagaagt
    22381 gcgtgaaagg cctcgtcaag tattgggcgc tcgtcgatcg ctacgagtag cggccctgga
    22441 tttgccgcgg cgtggacgat cccgccatcc cggaacctgt gataagagcc gcgcgcggtc
    22501 gcagtcaggc cgtggcggga gacgtatcca tgggcacggc cctcttcatc accggcgcac
    22561 tcgtcggcgc ggcgctcatc gccttgctcc tcgcaaccct cgcgcgccca gcgctgcgca
    22621 tctggccgcc gcccgggccc ggcaccgccc aaagctacgt cttctggatc ctgttccgct
    22681 cgctgaacat cctctgcttc gccgtcgccg tgatgaaccg cgaagcctat ctcgggctgc
    22741 cgctgtggct tcgggttgtg gccttcgcgg ttctcgtcgt atctgtcgcg accttcatct
    22801 acgccttcaa gatgctcggc cgtgacaaca gttacggcgc aagcgagggc ctcgtcaccg
    22861 gcggcatcta tcagtggacc cgcaatccgc agaacgccat gcttatcgtc gtctatggcg
    22921 cgctcgcgat cgccgctgac aacggcccga cctacgtgct gtgtttggcg atgatgctcg
    22981 tctacacact gatggtgctg ctcgaggagc cctggctgga attccactac ggcgatgcct
    23041 accgccgcta ctgcgagagc gtgccgcgct tcttcaactg gcggcgcgcc ttcgggcggt
    23101 cgtgatcgcg cacccggcac tcgcgaaacg tcagcaaatt gttgacattt tatctcggtc
    23161 gcgagaggct cgtgatcgtc ggtcagtcat ggcgaccggt gagcgggcgc cacgtgtgga
    23221 gtgggcatgg gcagcgggga gccgcgacga ctggtcggcc ggcgcaaggg cgccgagcag
    23281 cccggcgcgt ccgcgacgac cgccaggacc accgggttcc cggtcggacc gattgtctcc
    23341 tccgcgcatc tcgccgccgg ggccgcacca ggattatccg aggtcgagtt cggcctgatt
    23401 ctcgcgtccc acgcgttcga gcgatggatg gtgcgctgca tggcggccgc cggtgtaggc
    23461 gacctgtcgc ctgtcgaggt cctgatcatg cacacggtgc gccatcgcga ccggccgaag
    23521 aagttggccg atgtctgcct ggtcctcggt atcgaggaca cgcacgtcgt caactacgcc
    23581 atcaagaagc tcacgacagc gaaactcgtc gcctcccacc gcgtcggcaa ggaaaaggtc
    23641 gtcgccgtca cccgcgaggg agccgaggcc tgcgcacgct atcacgagat ccgcgaacga
    23701 ttgctgatcc gcgccgtcaa atccaccgga ttgccagacc cgatgctctc cgagcttggc
    23761 gcactgctgc gggcgctctc cggacactac gatcaggcgg cgcgatcggc gacgacgctc
    23821 tgacagatca ctgttggcag cgacgcctga acccgggcgt cgataggaag ggagaccgaa
    23881 aatgctgaag agcgtgctaa cgggcctgct cggcgcgacg ctgcttgcag cgccggccat
    23941 ggcgcagacc aagtgggaca tgccgacacc ctatccggac ggcaatttcc acacgaagaa
    24001 cgcgcgggcc ttcgccgagg aggtcgagaa ggcgacgggt gggaagctca aaatccagat
    24061 ccactcggcg ggctcgctgt tcaagcaccc agagatcaag cgcaacgtgc gccaggcgct
    24121 ggcaccggtc ggcgaggtgc tcatttcgct gcatgccaac gagagcccgt tctacggtat
    24181 cgacgcgatc ccgttcctgg ccacgtccta tgcggagtcg gcgaagctct acgccgccaa
    24241 tcgtccattc ctcgagaaga agctcgctgc cgacggcctc gtgctcctct actcggtgcc
    24301 ctggccgccg cagggcatct acgccaagaa ggagatcaag tcgatcgacg acctcaaggg
    24361 cctcaagttc cgcacctaca atccgggcac ggcgcgcatt gccgcgctcg tcggcgccgt
    24421 gccggtacag atcgaggtgc cggatctgcc gaccgccttt gcgaccgggc gcgtcgaagt
    24481 gacgatcacg tccggatcga ctggtgtaga ggcgcgcttc tgggatttcg tctcgcacta
    24541 tcacgacacg cgcgcctggt tgccgcgcaa cgtcgtgttc gccaacaagg cggccttcga
    24601 caagctcgac gccgaccaga agaaggcgct gcttgcggcc tcggcagctg ccgaagcgcg
    24661 tggcttcaag gcctccgaga ccgagacggc ggagcgcatc gccgcgctca aggccaacaa
    24721 gatgaatgtc gtcacccctt cgcccaccct gatggacggc ttgcgcaagg tcggcgcgca
    24781 gctcgctgcc gagtgggaga aggccgccgg cgcggacggt gcggcgttgc tggctgccta
    24841 ccgaaagtaa ggccctcgcc gccgtcctgc gtcagcaaga cggcgccgtt caggcttgcc
    24901 cgagcgccgt ggtgacgctt gccgcggcgc atcggtctga tggctgtggc ctgactgctt
    24961 tggtcagttt gcttggggga agcggtgcgt gctgttctcg atggagtcta cgccgctacg
    25021 ctgcggctct cggcgacgtg cctcgtcgca atcgcagtgc tggtgctggc tcaggtgctc
    25081 gggcgcctcc tggatatcgc gttgaagctc cttggcctcg cgcccgtcgg cttcatcatc
    25141 ctctcgctca gcgagattgc cggctatctg ctcgctgccg cgagcttcct ggcactcgcc
    25201 ggcagcctga agggcggcgc gcatatccga gtcaccatgc tgctcggtgg cgtcggcgag
    25261 cgcctgcgcc gcgtgatgga gctgatcgtc ttcatcaccg ccctgctctt cacagcttac
    25321 atgacgtact acgtcgcgac actcgccttc gacagctggc ggcacaacga aatttcgccg
    25381 ggcctcgtca aggtgcagct cgtttggccg caggcggcga tggccgtagg gctcggcgcg
    25441 ctcgtcgtcg ccctcgccga tgagatcggc atcctcatcc gggagggacg gccgagcttc
    25501 cgcgcagccg aggacgccgt gaccctcggc aaggaaggct gacgccatgg acctcatcac
    25561 gctcgccttc ctcctgatct tcgccctgtt cgccgtcctg gcgaccggca tgtggatcgc
    25621 cctggcactt gccgccatgg gcatgctggc gatgatcgtg cagagttcgg cccctgccgg
    25681 ccaggtcatg gcctcggcgc tctggagctc gctcaattcc tgggatctga ctgcgctgcc
    25741 gatgttcatc tggatgggcg agatcctcta tcggacgaag ctgtcctcgg acatgttcga
    25801 ggggctcgcc ccatggctgt cccggctacc cgggcgcctg cttcacatca atgtcgccgg
    25861 ctgcaccatc tttgccgcgg tctcgggctc gtcggcggcg acctgtgcca cgatcgggcg
    25921 catcgcactc cccgagctca tgaaacgcgg ctacgacgaa cgcatggcca tcggcacgct
    25981 cgcgggcgcg ggcacgctcg gcctgctcat cccgccgtcg atcatcatga tcgtctatgc
    26041 cgctgcgacg gatcagtcgg tggcgcgtct cttcatcgct ggcgtcatcc cgggactggt
    26101 gctcgccggc ctgttctccg gctacatcat cgtctgggcg ctgttcaatc ggcaccgcat
    26161 gccgcctgcc gacccgccgc agtcgttcgc gagccggatg gccgccttgc gccgcctgct
    26221 gccggtcgta ctgctcatcg tcggcgtcat cggctcgatt tacggcggcc tcgcttcggc
    26281 gaccgaggcg gccgtcgtcg gcgtcgcgct ttcgctgctg ctctcgtacc tgatgggaac
    26341 gctcagcaag gccagcttca gcgaggccct gctgtctgcg acgcggacgt cgtgcatgat
    26401 ctgcttcatt ctcgcgggcg ccgcattcct gaccacggcc atgggcttca cagggatccc
    26461 ccgcgcgctc gccgagcatg tcggctcctt caatctgccg agctggcagc tgctgacagc
    26521 gctcaccctc ttctacatca tcctcggctg cttcctcgac ggcatttcga tggtggtgct
    26581 gaccacctcc atcatcatgc cgctgatcga aaaagcgggc ttcgacctga tctggttcgg
    26641 aatctatgtg gtcgtggtcg tggagatggc gcagatcacg ccgccggtcg gcttcaacct
    26701 ctacgtcctc cagtcgatga ccggacgcaa cctcttccag atcggcgcct acacgctccc
    26761 tctgttcgcg ctgatggtcg tgatggtcat cctgctcgcc gtcttcccgg ggctggtcac
    26821 ttggctgcct tccaccatgc tcgacacgag atgatggccc gacggtgatg ccgcccgctc
    26881 gcaagatgcc ttgacgcgac gaccaaagat cgctgcactc tcgagcctcg cagcgatcgg
    26941 agctctgcat ggcggacttg cacgatcatg agacgctgga tgccgtctgg cagcgcgccg
    27001 tcgtcgagca tcgcgatcgc ccgttcctct gcgtgccggc gagcgcctcg cgagactatg
    27061 cgaccggcgg cctcgagctc acctatgccg ccgccgccgc cgaggtcgat cgcctcaccg
    27121 aggcttatcg cgccgcgggc tatggtctcg gccatcgcgt ggccctactc ctcgacaatc
    27181 gccccgagca cgtgctgcat cgcctcgcct tgagccgcat cggcacctcg tgcgtgccga
    27241 tcaatcccga ttaccggccg gccgagatcg cctatctcgt cgagcactcc gagccggaac
    27301 tcgtgctggc gttggcggcg cgcgaggagc aggtcggcgc cgggctcgtg atgtcgagcc
    27361 acaagcccca cctccgcctc ttggaagctg gcgccccgcc gccgccacgc tcgcaggctc
    27421 ggccgggcga ggtcaccgcg gcaaccgagg ggcagatcct ctacacgtcc ggcaccacgg
    27481 gccggccgaa gggctgcatc ctctcgcagg gctacgaggt cgcctccggc gcctggtatg
    27541 cgagcctccc tggcatggca cggctcgggc gggcgcgcga gcgcatctac aatcccctgc
    27601 cgctctatca cgtgaacgcg ggggtcgtgt cgcttatcgg cgcgatcgtc ggcggcaatt
    27661 gccagatcct tcccgatcgc ttccacccgg agcgctggtg gccggaggtg agcgcgacac
    27721 gcgcgagcgt gatccactac ctcggcgtcg tcatccagat gctcatgaag cgcgcnnnnn
    27781 nnnnnnnnnn nggcgtacgc ttcggcttcg gcgcaggtgt cgagccgcaa ctgcatgccg
    27841 cattcgaagc gcgcttcggc ttacctctga tcgaggtgtg gggcatgacc gagatggtgc
    27901 gcgtcttgaa cgacagcgtc gccccgcgtc agatcggcac gcgcgcgttc ggccggccgc
    27961 agcccggcgt cgagatccgt gtcgtcgacg atcacgaccg cgaggtgccg gccggcacgc
    28021 ccggcgagat gacgctccgg cattcggcgg cgacaccccg gcgcggcgcc ttctcgggtt
    28081 acctcaagaa cgcggaggag acggagcggt catggcgcgg cggctggttc cataccggcg
    28141 acgtcgtacg ccaggacgcg gatggcatgc tgcacttcgt cgaacggcgg aagaacatca
    28201 tccgccgctc gggcgagaac atcgccgctg ccgaggttga ggcgacgctg ctcgagcatc
    28261 cgggcgttat gcaggtcgct gtgctcgccg ctcccgacga agtgcgcgag gaggaggtgc
    28321 tcgcctgcgt ggtgccgcgc acgggcgagg gaagcgaagt gctcgccgat gagctctttc
    28381 agctctgctt cgagcggctc gcctatttca aggcgccggg ctggatctgg ttcgcgccga
    28441 gcctgccgac caccggcacg cagaagatcc agaagcacgc gatcttccca ggcctcgacg
    28501 atccgcggac ggccgccggc atgcgcgatt tccgggctcg caagcggagg gggtgagagg
    28561 cgccatgcaa atagggaatc gcgggtctgg ggcgcgcagc cccaggcgga tcgggacctg
    28621 cggccccgtc gcgtagcgaa gccattctcc cgaagcgcat cgctcgccta tggggccacg
    28681 cccaactctc cgagcatgca cggtgtcagc cccttgggtc tgacaccgac tgcacggagg
    28741 ctcagaacag cccgtcgatc tggcccttct cgttgaaccg gatgtgctcg gacgagggca
    28801 ccttcggcag gcccggcatg gtcatgatct caccgcagat cgcgacgatg aagcccgcgc
    28861 ctgccgagag gcgcacctcg cgcacaggga tcgtgaagcc cgtcggcgcg ccgcgggcgt
    28921 tcgggtccgt cgtgaacgag tactgcgtct tggcgatgca gacgggaagc ttgccgtagc
    28981 ccatctcctc ccaggtcttc agctggtcgc ggatggactt gtcggcaacg atgccgtcgg
    29041 cgcggtaaat cttcttggcg atcgcctcga tcttctgggc gagcggcatg tcgtcgggat
    29101 acagcggcgc gaacttcgag gtgccgccgt cggcgatctc ggcgacgcgc ttggccatcg
    29161 ccgcgatgcc cttgccgccc tccgcccaat gcttgttgag gaagcactcg gagcccaagg
    29221 acttcacgta gtccatcacc gcggcgacct cggcttcggt gtcggtcacg aagtggttga
    29281 tgccgacgac ggccggcacg ccgaacgatt tcacgttctc aatgtggcgg ccgaggttgg
    29341 cgaggccctt cttgacggcc tccacgttct ccttgccgag gtcctccttc ttcacgccac
    29401 cgttcatctt catcgcacgg acggtcgcga cgatcaccgc gacgtcaggc ttcaggccgg
    29461 ccttgcggca cttgatgtcg aagaacttct cggcaccgag gtcggcgccg aagccggctt
    29521 cggtcacgac gtagtcggcg agcttcagcg ccgtcttcgt tgcgacgacc gagttgcagc
    29581 cgtgggcgat gttggcgaac gggccgccgt gaatgaaggc cgggctgttc tcgagcgtct
    29641 gcacgagatt gggctgcatg gcctgggcga gcagcaccgt catggcgcca tccgccttga
    29701 tgtcgcggca gaacacgggc gtcttgtcac gacgatagcc gatgatgatg tcgccgagcc
    29761 gcttctcgag gtctttgagg tcgttggcga ggcagaggat cgccatcacc tcggaggcga
    29821 ccgtgatgtc gaagcccgct tcgcgcggga agccattcga gacgccgccg agcgagcaga
    29881 cgatctcgcg cagcgcccgg tcgttcatgt ccatgacgcg gcgccaggcg acgcggcgcg
    29941 tgtcgatgtn nnnnnnnnnn nnnnnnnnnn nnagatgtga ttgtcgatca tcgccgagag
    30001 caggttgtgc gcgctggtga tggcatggaa gtcgcccgtg aagtggaggt tgatgtcctc
    30061 catcggcacg acctgggcat agccgccacc ggccgcgccg cccttgacgc cgaagcaggg
    30121 gccgaggctt ggctcgcgca ggcagatggc ggctcgtctg ccgatgtgat tgaggccgtc
    30181 gccgagaccg accgtcgtcg tcgtcttgcc ctcgcctgcc ggtgtcggat tgatcgcggt
    30241 gacgaggatg agcttgccgt ccttcttcgc ggcgagcgat ttgatgaact cctccgagat
    30301 cttcgccttg tcatggccga aggggagaag gtgcgccgtc gggatgccga gcttcgcgcc
    30361 gatgtcctgg atcggcatct tcttcgcctc tcgcgcgatc tcgatatcgg agaggtactt
    30421 cttggccggt gcggcggctg acgccatggg cttcgcctcg tttgctgggg tacgcggtgc
    30481 aggagcggag aannnnnnnn nncgaggatg tcgttggcct tcgcccagtc ggcggcggcg
    30541 atcttcttgc cgtcggcgcc gcggacgcgc ttcacgacga tgcggtgatc gtcacccgtg
    30601 ccgacggtca cgccgtcgcc gttgatcgag aggacctcac cggcgatgcc gggtcccttg
    30661 atcctcgcgc tgtcgaaaat gtcgaccttc ttgccggcga tcgtcgacca ggcgcccggc
    30721 gcgggattgg cggcgcggat cgtgttgtag atctcggccg acggcttcga ccagtcgagc
    30781 tccgcgagtt ccttcttgaa ccagctctcg taggagccga gcttcggatt ggccttgagg
    30841 ccctggtcga tcttcggcgc cttccctgcc ttcacgaggt cgaggctctc gatcatcgcg
    30901 tcgacgccga tcgggaagag cttcttgaag tagacgtcgc cgagggtctc gtcgggaccg
    30961 atgtcgaccg tcttctgcaa gaggatcggc ccctcgtcga gcccctcgtc cggccagaag
    31021 atggtgaggc cggtcttggt cttgcccatg gcgatcggcc agttgatgga cgagggcccg
    31081 cgatgatccg ggaggagcga agggtggtac tggaaggtgc cgaagcgcgg cgcatcgagc
    31141 accggctgcg gcacaaagag gagcacatag gccatcatgc agacgtcggc cttgaagctc
    31201 ttcatgagct cgagcgcctc gggcgtcttc cagctcttcg gctggtgcac agggagcccc
    31261 ttcgccttgg cgaacagctt cagcgggtct tcagggcgac ccgccttgtc cggcgcgcaa
    31321 cagaccgcga cgacgttctc gccgcgctcg aggagcttct tcaggacggc ctcgccaaag
    31381 gcctcctgtc cgtgaactac gattcgcatt ctggcttcct cccagtgcct ctggaaccag
    31441 tctctgctgg gctgcgaggc gatcgttgaa attatgttgg ctggacctgg tgcgacgtgc
    31501 agcgactatt ccgccgccgc cttcttcggc gcctcggtcg caccagacga cttgatcgcg
    31561 gccatctcgt cggccgagta gccgagcaca gttgccagca cctcgtcggt gtgctcgccg
    31621 agcagcggcg agcgcgtgac gacggtcggg ctcgccgaca tcttgatcgg gttgccgacg
    31681 gaaaggtact tgccgcgctt cggatgatcg acctcgacca ccgtaccggt cgcgcgcaga
    31741 gacgtatcct cggcaagctc cttcatcgac aggatcgggc cgcaggggat gtcgtacttg
    31801 ttcaggagct ccatggcctc gaacttggtc ttggtcatgg tccaggcctc aatgcgcccg
    31861 aagatttcat tcagacgggg caggcgcgcc ttgggcttcg cgtagtcagg atgcgttttc
    31921 cagccgggct cgccgatgac gtcgcagatc gcctcccaga cgggcgcctg cgtgatgaaa
    31981 tagatgtagg cgttgggatc gctttcccag cccttgcact tcaggatgcg gcccggctgg
    32041 ccgccgcccg agtcgttccc cgcccgcggc gtcgcctcgc cgaacgggat gccctcaccg
    32101 aactggctgt actcggtgag cgggccatga gagagccgct gctgatcgcg gagcttgacg
    32161 cgcgcgaggt tgaggacacc gtcctgcatc gcgcacatca ccttctgccc gaggcccgag
    32221 tgcgtgcgct ggaagagggc ggcgacgatg ccgagcgcca gatgcagacc cgtgccgctg
    32281 tcgccgatct gcgcgccggt gaccagcggc ggaccatcac ggaagccggt ggtcgaagcg
    32341 gagccgccgg tgcactgggc gacgttctcg tagaccttgc agtcctcgta tggaccgggg
    32401 ccgaaaccct tcacggaggc aacgatcagc cgcgggttga tcttgtggat cgtttcccag
    32461 gggtagccca tgcggtcgag cacgcccggc gcgaagttct cgacgagcac gtcgcacact
    32521 ttcatgaggc gctcgagcac ctctttgcct ttggggtgtt tcgtgtcgag cgtgatcgag
    32581 cgcttgttgt ggttgagcat cgtgaaatag aggctgtcga cgttcggcac gtcctgcagc
    32641 tggccgcgcg tgatgtcgcc gacacccggc cgctcgacct tgataacgtc cgcgccgaac
    32701 caggcgagaa gctgcgtgca ggtcgggccg gattgcacgt gcgtgaaatc caggatgcgg
    32761 acacctttga gcgcttgagt catgtagcgg ttccccgagt gtgttggctg ttatcagaac
    32821 gtgttggtgc ggtagatttc agcgagcggc agcgagatgc cgacgctctc cagcgtcagc
    32881 gtgtcgccag gaaagtgctc ttcgacgagc catgacgtgc gtcgccgcag gatctcgacc
    32941 atcggccggt cgtgcgcgac gagcacgaac tcctgaagcg tcgggatctg cagatacttg
    33001 aggcgcttct cgcttcggtc gaggcgcccg gtcgatggcg acagcacctc gatcagcagg
    33061 atcggctcgc ggcggaacat cctgtcgcga tcgcttgccg cgcacgagac catgatgccc
    33121 gggtagtaga cgtcgctgtc ccgcgcccga tcgagatctg agcaggtcgc taagacgtca
    33181 ggatagtagt aggcgtcgtc ggaaagcgcg cggagcttca agcgcatgtc gcccatgaag
    33241 acgtcgcagc gaggcggcag cacgccgtga gtggccgcga aaagattgcc ggccaatcgc
    33301 ccatgatcgt tcgtcgcgcc gcccctggcg tagataacgc tatcgcggaa ctcgtggcca
    33361 atgggcgacg cggcatcgac ggcgagatag tcctcgaccg tcgatcgctt caactctcgg
    33421 atggcgctca tcagagcctc ctctccgtcc acaccagaga ccaggacgtg ctccgcggtt
    33481 ctgcaggctc acgccttctt ggcaacgacg ctctgcgggt tgaggttgcc gatgcggccg
    33541 ctctctgtgc cggccttctc gtcgatgatg gcattgatga gtgtcggctt gccgctgtcg
    33601 atcgcctcgt tgaccgcgcg cgagagctcg tcgggcgagg tcgcgtacac accgacaccg
    33661 ccgaaggcct tcgccatcag ctcatagcgg ctgtccttga cgaaggtcgt ccaggccggg
    33721 tcgtcgccac ccttgttgac gtcggtgccg cgatagatgc cgttgttgtt gaagacgacg
    33781 acgcagaccg ggaggttgta gcggcagatg gtctcgnnnn nnnnnnnnnn nnnnnnnnnn
    33841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33901 nnnnnnnnnn nnnnnnnnnc cccaggtacc gacgtcgatg cgcttccgcg gcttgtagat
    33961 gtcgatgatg gagcgcgcga agtcgagcgt gttggcgccc tcgttgacga ggatcgtctc
    34021 gggccgctcc ttcatcacgc ggcggagaac gccgagcgcg ccgtggtagt ccatcggcac
    34081 gttgttgttc tggaggcgcg gcgccatctt ggcgacgttg ccctcgacct tgccgcggat
    34141 gtcggccatc catgcaacag gcttcggcca gtcgctgccg aggcgcgaga gcatgagcgc
    34201 gacgctcgag gcgatatcgc cgacgatcgg cgcatcgatc tgaacgttgc tgtccatctc
    34261 attgggcgag atgtcgatgt gcacgaactt ctgcttgccg cgctcgcccc aggtcttgcc
    34321 cttgccgtgc gagaggagcc agttgagccg cgcgccgatc agcatgacga catcagcatc
    34381 cttgagcacc atcgagcggg ccgcgccagc cgactgcggg tgcgtatcgg gaagcaggcc
    34441 cttggccatg ctcatcggca ggaacggaac gccgctcttc tcgacg
//
LOCUS       DAORNH010000013        33255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1019_length_33255_cov_2.583163, whole genome shotgun sequence.
ACCESSION   DAORNH010000013 DAORNH010000000
VERSION     DAORNH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 33255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33255
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1019_length_33255_cov_2.583163"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /gene="trmFO"
                     /locus_tag="PK264_02585"
     CDS             complement(<1..757)
                     /gene="trmFO"
                     /locus_tag="PK264_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRD74816.1"
                     /translation="MSIDRPVHVIGGGLAGSEAAHQLARGGVPVVLHEMRPVRGTNAH
                     KTDGLAELVCSNSFRSDDTYNNAVGLLHEELRRSGSLIMAAGDRHKLPAGGALAVDRD
                     EFSAEVTRRLVSNPLVTIAREEIAGLPPPEWDSVIVATGPLTSPALGAAIGGLTGEAD
                     LAFFDAIAPIVHFESIDLSIAWRQSRYDKAGPAGTGADYLNCPMTKPEYEAFVDALLA
                     GEKTEFKEWEGTPYFDGCLPIEVMAERGRDTLRF"
     gene            934..2451
                     /locus_tag="PK264_02590"
     CDS             934..2451
                     /locus_tag="PK264_02590"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD74817.1"
                     /translation="MAKGIRYLFTLGVPAALAIAGSAYVLGMRPQGGGPAYTYDTVTV
                     GKGQIRKLVSSSGPVRALVTVSIGSQLSGQIAELKVDFNAEVKAGDVLAVLDNKTFAA
                     KVAQAKADLATAKALLTAALEKAEAVLKSAERVAARQSTLAAKGIAATVTVDNATRDM
                     DVAKADIAVAKAQIESARAQIAQREAQLEQAMIDLERTIIRSPIDGTVIGRTIDIGQT
                     VAASLQAPELFKIAQDLRRIRIEAQVNEADVGAVAEGNTVTFTVDAYPELRFTGLVTQ
                     VRLAATELQNVVTYTVIIEAANAERKLFPGMTANVQIETAKRDGVARIPMDALRFKPR
                     NAAAASAGAPAATTRASPEERERRMAELTASLKTDIGLSEEQIAKVKAELAKGFQGRR
                     SGAGAGSGGPSAGGDGPGGGAAPDPAQIRARMLARTAEAVASVITSDQRPAFEAWRRT
                     RETARTASIWLLGETGQPVSRQIRIGIADDQFAEVLGETVKDGERVIVRAREVKK"
     gene            2448..3215
                     /locus_tag="PK264_02595"
     CDS             2448..3215
                     /locus_tag="PK264_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74818.1"
                     /translation="MSDASTSGAPRPASGAPPLIGARGISKVYRMGDQTVRALDDVSL
                     EIRSGEFVAIMGPSGSGKSTMMNLIGALDVPTSGVLEIDGRDIAKQSSDELADLRNRT
                     IGFVFQQFNLLPRTDALRQVMLPLAYARPRPTNAEELARHRLEQVELGNRLDHHPRQL
                     SGGQQQRVAIARALVNAPKLLLADEPTGALDSRTSEEIMRLFCRLNDEGITVVLVTHE
                     MDVAAYAKRRIMFRDGKIIEDIAQPGVPLAAHPRAAE"
     gene            3212..4447
                     /locus_tag="PK264_02600"
     CDS             3212..4447
                     /locus_tag="PK264_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD74819.1"
                     /translation="MNPLESFKIALLALRTNLLRSILTTLGIIIGVGSVIVMVAVGAG
                     ARSEVDRQIAALGSQTLNVIPGSSRVMGRSGGSGTTLPISEGDLKAIRDKIPGVVAIS
                     GLLGGSAQVVRGNTNWTTSINGVHDEFFTVREWQVLDGREFTKDEMRSGARLAVIGQT
                     VALKLFGDGASPLGQTIRIKNVPFTVIGVLGPKGQSSFGSDQDDVIMLPITAARGRIV
                     GRSQVVNDSIGRIQVKFDDDVNLAEAQEAIEQLMRQRRRIQPGAEDDFFVRNFAEFMR
                     ARTAALSTMTWLLAATSAISLIVGGIGIMNIMLVSVTERTREIGLRMAVGGRRRDILM
                     QFLVEAVTLCLLGGLIGVMLGGIVAFIVATSAQWPILISKEAILVAMAAAAATGIFFG
                     FFPARRAAYLNPIDALRSE"
     gene            4553..4777
                     /locus_tag="PK264_02605"
     CDS             4553..4777
                     /locus_tag="PK264_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74820.1"
                     /translation="MSKSLWIAGAAALLSVATVAGVSAAGPMSPITGVKPASPSLVET
                     VHCRRYFHCHRVCRWGRYRIWCWRACHRCG"
     gene            5058..5384
                     /locus_tag="PK264_02610"
     CDS             5058..5384
                     /locus_tag="PK264_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74821.1"
                     /translation="MRSQLAAVAAPKVLDNPSAPLARDAVAAHFVVVAQAEPGLLPRL
                     VEPVAKLGFVPSRVHASREAGDGSEMTVDLRLTDVPARTAELVGMALRAIVGVHRVSA
                     VVEPQH"
     gene            5472..5672
                     /locus_tag="PK264_02615"
     CDS             5472..5672
                     /locus_tag="PK264_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74822.1"
                     /translation="MPIAVAAALLGAGLYAGYRLYSSLQERMADELARAEADHRRQRG
                     EKDLGRLEFDPASGVYKPSRRG"
     gene            6283..8271
                     /locus_tag="PK264_02620"
     CDS             6283..8271
                     /locus_tag="PK264_02620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRD74823.1"
                     /translation="MHQQRAVFRRDIEALRAISVLAVVLFHMRVPGFGGGFAGVDVFF
                     VISGFLISGQLLRERESTGGIDLLAFWSRRAWRLLPNALLVLSVTAVVAAFIMSADQT
                     GPLALDIASASVYMANYRFATRAIDYFDNAQHASPVLHFWSLSVEEQFYLIWPCLLLL
                     ACWRAAGDRIRRRAATIAALIIIASFVASAVYIHRNQPAAFFHTEARIWQLAVGAILA
                     VLDDRAILPERLRTLASGAGLLGILGSVTLLNERDYPGSAALVPVASAALLIAAGRSG
                     SEAGVQRVLNLAPLQWLGSRSYSLYLWHWPILLLGKSVEFGPMEALGLMGLSIAVAAT
                     AYAVMEAPLRRYGMRQQRSVQGLAMPLAASGCIGAVAVGLAVVGMSSFNPFVSEKAQQ
                     IAQLIARAREDGPQIGAVGRCRATIDNDPRSGCRFGAPNSERTVVLFGDSYAEHLFDG
                     MDAAAREAGWSMRIWTRAGCPPIDAPSLDSGKRVVDADCLSWRNAVVRQLIADAPRLV
                     VVSSWSGVATNLADDSGRALSRGQSRLAWREGFASLLGRLRRGGVEVAVVRGTPRGSF
                     SELHSCLLVGTATSDRCGRPRGNATAYSSLDLEVAATVAGVRLVDLADRFCGPNVCYS
                     FRDGMIVYRDRHHHLTAAFSRSLSSDFKRLLQDVELGR"
     gene            complement(8315..9292)
                     /gene="corA"
                     /locus_tag="PK264_02625"
     CDS             complement(8315..9292)
                     /gene="corA"
                     /locus_tag="PK264_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HRD74824.1"
                     /translation="MLTLYDTRGQTPGLARREGCDTILGTTVWLDLLNPTEAEDEHVE
                     KALGISIPTRAEMREIEASNRFYQENGAHFMTAFIVYNVDAPIPETSTLTFVLAGNRL
                     VTVRYSEPKAIQLYVARADKGDAQCSSGATILTGLIESIISRQADLVERIQDETERLA
                     RSVFEIKGGQQTRSRRLDVLLKTTGKEGDLVSRAQESAFSMDRLLTYFLAVAKERGDD
                     PRLVERLQTAQRDTASLMEHMRFVSNRLTFLLDATLGMINIEQNQIIKLFSVVSVVLM
                     PPTLIASIYGMNFKAMPELSWPLGYPWAIGLMIASAIVPFLYFRRKGWL"
     gene            complement(9404..9937)
                     /gene="ppa"
                     /locus_tag="PK264_02630"
     CDS             complement(9404..9937)
                     /gene="ppa"
                     /locus_tag="PK264_02630"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRD74825.1"
                     /translation="MRIDAIAVGNNPPHDVNVIIEVPIGGEPIKYEMDKAAGTLVVDR
                     FLHTPMRYPGNYGFIPHTLSEDGDPIDVVVANTRPIVPGAVMNVRPIGVLMMEDDKGM
                     DEKIVAVPSPALTKRYEKVTSYKDLPLITIEQIQHFFEHYKDLEPGKWVKLKGWGGPD
                     DARRMIVEAIERARRQG"
     gene            10170..11228
                     /locus_tag="PK264_02635"
     CDS             10170..11228
                     /locus_tag="PK264_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74826.1"
                     /translation="MTLSAKAYDLNDVEAVQEHFHARGWTDGLPIVPPTPKAVEACLA
                     WAGLAPDQLIGIEPVRAQPVTAEKLAINAVMAGCLPMHFPVVVAAIAAMLREDFLVHG
                     ATASTGGSAVLLVINGPIRLEIGASGGFNAIANSDRATAAIGRALRLVLINILDVRPG
                     EIDRSTLGHPGKFSFCIAEDEEGTGWTPLAETRGVPKGASAVTVMAACAPRQIMNEWT
                     TKPEEILETFAAEMRANLRHYSIHPGNYAIVIPKQIRNHIEAAGWDKARIATFIHERA
                     RIRRKEWAEVGKAAVVRDRGESVYTALERPEQLLVIAAGGPAGGFGAIIPPWLGHKSR
                     AVTLPIGACIDCEPPKKA"
     gene            11448..11882
                     /locus_tag="PK264_02640"
     CDS             11448..11882
                     /locus_tag="PK264_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD74827.1"
                     /translation="MRAHIAIVEDDDHLALMLHYNLTTRGFAITRFKDGIEALASLTS
                     APPDAVVLDWQLPRLSGIEVLRHLRATPATAELPILMLTGRTLPEERVRAIALRVEAF
                     ITKPFSVSDVLDRLMRALAGRGAQEPGPVMPSARREGGIGCG"
     gene            12337..13182
                     /locus_tag="PK264_02645"
     CDS             12337..13182
                     /locus_tag="PK264_02645"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HRD74828.1"
                     /translation="MQYRPPPRATAEIRRYRAIFISDIHLGTRAAQSAALLEFLRLCD
                     ADTYYLVGDIVDFWKVRRGPYWPQSHNDVIQKLLRKVRKGSKIVFIPGNHDECMRDYC
                     GMHFGGVDIERACIHRTANGRRYVVMHGDDFDVVVRTARWLAFLGDRGYEAALWLNNP
                     LNWLRRRCNLGYWSLSAFLKLRVKRAVSFIGAYEEAVATEARRQQADGIICGHIHQAA
                     DRMIDGVHYMNCGDWVESCTAIVETHEGRLDLVHWPHDAAMSDDAPISAEAPPHYVED
                     LRGIT"
     gene            13179..14426
                     /locus_tag="PK264_02650"
     CDS             13179..14426
                     /locus_tag="PK264_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HRD74829.1"
                     /translation="MTSESVASDMTAAGLLSRAVHRNRALSLDGALERLFTAAFSDLV
                     YPMIWEDPRVDLAALEIEPSTRIVTIASGGCNVLNYLTADPAEVHVVDLNPSHVALTR
                     LKLAAARHLVDHEQFLALFAKGEGAGNAEIFDRVLAHRVDADTRAYWTSADGWRRRKI
                     DRFVRGFYRQGLLGRFITLAHGVARLYGQDPRRMMQARSRTEQVAIFEAELAPLFDRL
                     LVRWILGHRAALYGLGIPPAQYAALLGGAGHMADVIRERLRKLACDFDIADNYFAWAA
                     FNRGLAAVGEASLPDYLDPANFAAIKSRTDRVLVEQASFTTFLSRQPISSLDRYVLLD
                     AQDWMSDAALTHLWSEIMRTARPGARVIFRTAGTTTILPGRVPALILDGWHYDATRSA
                     TLTKEDRSAIYGAFHLYIRNSRA"
     gene            14436..15095
                     /locus_tag="PK264_02655"
     CDS             14436..15095
                     /locus_tag="PK264_02655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD74830.1"
                     /translation="MDIAHQQVLDSNYRFQRHVYNVTRRYYLLGRDRLISELAVPPEA
                     NVLEIGCGTARNLVRLAECYPEAQFWGIDLSCAMLATAGKAVARRGLSGRVTLAHADA
                     ACFDPRLTFGRETFDRIVFSYTLSMIPDWQLAIKQAAACLAPSGSIQIVDFGSGRRLP
                     ATVRRALRAWLRRFHVTPRDDLVPFLGRVAEGGQYELFAEETYRTYAVRAVLTSAPSV
                     G"
     gene            complement(15128..16114)
                     /locus_tag="PK264_02660"
     CDS             complement(15128..16114)
                     /locus_tag="PK264_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRD74831.1"
                     /translation="MASRSLTSMLSILLATCVILTAPPTQTVANPATTAIETLRNVRT
                     WQFQLQHLGLSELSQSPADMLVIDMSRDATIEGAFTRREIEALQKGPGGRRRLVIAYL
                     SVGEAEDHRFYWRHYWPAAKPDWLLEENCRWETNFLVKYWSDEWKHNLFRGDDSLLAQ
                     IIHAGFDGIYLDRVDAYWDIRRRFPASRTEMIRLVSELRRKARAMKPDFLFIAQNAES
                     LLEDRTYRTLIDAVAKEDLLFGLTGTGRRNTRSSIAWSLGMLRRLKAERKPVLVVEYL
                     RSAEAIAGAERELRSLGLIPTFPTRLLDGRNPSAANVHSDVGTPEFARSNCQ"
     assembly_gap    16317..16333
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16334..17099)
                     /gene="cysQ"
                     /locus_tag="PK264_02665"
     CDS             complement(<16334..17099)
                     /gene="cysQ"
                     /locus_tag="PK264_02665"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HRD74832.1"
                     /translation="MSDLASDRLVERLLPTVLAAGALELRYYEAGVATELKSDETPVT
                     AADREAEAVLLEALARVAPGIPVVAEEAWSAGAATTPGDTFFLVDPLDGTREFIEKRR
                     EFTVNIALIEAGRPRFGIIYAPALGMLAATFGPGDARETLVHPGDTASSVTGLAWRAM
                     RTRRAPLAAQVAVASRSHLNAETAEMLDRYRVASTRNAGSSLKFCLVARGEADIYPRL
                     APTREWDIAAGHAILSAAGGSVTMVDGSPLAYGKVSA"
     assembly_gap    17444..17453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17454..17730)
                     /locus_tag="PK264_02670"
     CDS             complement(<17454..17730)
                     /locus_tag="PK264_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74833.1"
                     /translation="MLGAPDIAARDLLGGAEINHGAGGARRAEGKTQELESCRGLLCR
                     VADDVERKCLRLGIVVVRQDLETVADGADRSDHIMADLAGNQRGKFQI"
     gene            17671..17799
                     /locus_tag="PK264_02675"
                     /pseudo
     CDS             17671..17799
                     /locus_tag="PK264_02675"
                     /inference="COORDINATES: protein motif:HMM:NF021595.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
     gene            17943..18065
                     /locus_tag="PK264_02680"
     CDS             17943..18065
                     /locus_tag="PK264_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74834.1"
                     /translation="MPPLDAMSIQPYYTACLAETARMRVAIRSDGEAIVIEARV"
     gene            18216..18917
                     /locus_tag="PK264_02685"
     CDS             18216..18917
                     /locus_tag="PK264_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD74835.1"
                     /translation="MRIVSTRDAWTGIANCRTCAVRQSVLFAGLEESDFNRIHRPIEQ
                     FSYLPGTTVYRSEESADWVYTIRVGLVKLLQYLPDGTQRIVRLMRTSDAIGLEGLVGA
                     RYEHHAIALHQTEVCRIPLSVVRELLAHKPAFSAELMARWQRALSESERVIRELNTGT
                     ARGRVVRLLLWLAESQGEATCDLFGREDLGALLGLTTETVSRSMAELKRQGHIIEPRS
                     NQFVCDVARLRSLVE"
     gene            19032..20261
                     /locus_tag="PK264_02690"
     CDS             19032..20261
                     /locus_tag="PK264_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74836.1"
                     /translation="MRYPSDLGGRYTPMYFLAALGAGGLAVSFFMYLMWLTPHPASPI
                     PTFGSLSSTWAEGTPVMRGLIGLSAAGIALFSVLHLRLLAWNLRQCTRWLATPAAEAL
                     RSGNAETQLMALPLALAMAVNVGFIFGAVFVPGLWEQRELLFPLAVLAFAAIGILAMR
                     MFLAFLGRNLAFGGFDCAKNNSLGQMLSVFTFAMVGVGLSAAAAMSQVKWIVAAAFAG
                     AMFFIVAAGILGVVFLVLGFRAMMEHAAAEETTPTLWMLIPILTVFGIAVFRLKMSLA
                     HTFNSPVTTGEIFALLLGIVAAQLLFGLIGWAVMSRVGYFAKYVSGGTRSPGSFALVC
                     PGVGLFVSGQFLVHAALVRLGLIELYSIAHGLVLLPLLALQVATIALFFKLSKKLMSA
                     APEAPPRAAPATLETAA"
     gene            complement(20281..23043)
                     /locus_tag="PK264_02695"
     CDS             complement(20281..23043)
                     /locus_tag="PK264_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD74837.1"
                     /translation="MTEESGLSPGHIQLMVNGREVAVTGGALRRLADVLRDDLGLTGT
                     KIGCNAGDCGACTVLIDSAAACACLVPVGQIAGARVTTIEGLATDPLGARLQQSFVAH
                     GATQCGICTPGMIMAARELIARSPRPDRGEVEDALGGVLCRCTGYEAIVDAVIAAVTQ
                     DAVSEPLMPVAGGAIGASIAKVDGEARVTGRERYGADGIPTDALHVRVIRSPHAHARF
                     RIGDLDQALARHPGIGRILTARDIPANGHGVYPTIKDQPVLADGLVRFRGEAVLALIG
                     EASAIAKIGEHELPIAYEPLPSTVGIDAARADDAPRVHAGKPGNVLADGSVTSGDVAG
                     ALGTSPHVVDFAVETSFVEHAYIEPEAGYAVRIGDTIELHVSTQTPYQHRDETALVLG
                     LAKEQVRLIPTACGGGFGGKLDLNVHPLVALGALLTHRPVAYVYSRPESMAATTKRHP
                     ARVRVRLGATDEGRITAFESHAELDTGAYASWGPTVANRVPVHAPGPYVVPHARTRGT
                     AYFTHAPPSGAFRGFGVPQSAIASEAALDMLADRLGMDWLEIRRRNALKAGDVTITGQ
                     RLDSSVGLLATIDAVEPYWRDWSQAATAYNQGDRRQRRGVGIACSWYGIGNTSLSNPS
                     EMEMGVDTSGHVTLYCGAVDIGQGSNTILAQIAADALGVPVATIALVTGDTALTRDAG
                     KTSASRQTLVSGKAAELAGRDLRARLFAVAGAPDGLEADPASQLSLANGEVVVASANA
                     HYRSALPPGIGNVVTGLGRFDPPTTPLDHSGQGNPYSAYAFATQVALVEVDLDLATTR
                     VLEIAAAHDVGRAINPQQVIGQIQGGIAQGLGLALMEEYVPGRTENLHDYLIPTFGDM
                     PRITALIVEAPTPHGPSGAKGVGEPALISTAPAIYGAIFKATGVRVTKAPALPHRLLA
                     AMRQRG"
     gene            complement(23040..23150)
                     /locus_tag="PK264_02700"
     CDS             complement(23040..23150)
                     /locus_tag="PK264_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74838.1"
                     /translation="MAELAPIDDIRASADYRREAALVLVGRTLSALGAPP"
     assembly_gap    23210..23301
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23302..23922)
                     /locus_tag="PK264_02705"
     CDS             complement(<23302..23922)
                     /locus_tag="PK264_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRD74839.1"
                     /translation="MAVSEGADSYFRPATLAEALGVRAAGAAIIAGATDVYPALVPGI
                     GRDLRPFGRGARASLDITGLAELRGIEERDGLWRIGAATTWTEIADARLPPAFACLRL
                     AAREVGGRQIQNRGTIGGNLVNASPAADGFPPLLALDAEVELQRRGHVRRLPLSRFVS
                     GPRTTALDPDEILTAILIRQSQRPARSHFLKLGARRYLVISIAMVAA"
     gene            24229..24819
                     /locus_tag="PK264_02710"
     CDS             24229..24819
                     /locus_tag="PK264_02710"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HRD74840.1"
                     /translation="MIALFFEVEPHPGHEQAYLDLAAKLRPELDRNGGVTFLNRYRSL
                     ARPARMLSHQYWQDRTHLDAWRNHAGHRGVQRAGRDRHFADYRLRVGAVVAELRPGSP
                     MQYLDGFEAARTGRRFVVAVEAKGSAVALHGGESFESVYVAGEMVSLSSFESLGEAIG
                     CIEAVVAGRGVVAARIAAVERDYGPRSRAEAPADAI"
     gene            24904..25362
                     /locus_tag="PK264_02715"
     CDS             24904..25362
                     /locus_tag="PK264_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HRD74841.1"
                     /translation="MTEIASRSASEASRIAEFLGLPRARSIDDLALVECVRKGFPVKT
                     ASVVVKRIDPDGRFLDPTQIVPKSTLSRRTKAQQPLSREESERILAISRVFAEVLRIY
                     RDDTGRSAQFLVHPHPLLGGETPMQLAVGSIAGAELVLKLLDRADAGVAV"
     gene            25362..25856
                     /locus_tag="PK264_02720"
     CDS             25362..25856
                     /locus_tag="PK264_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HRD74842.1"
                     /translation="MVARKLERALRVFRIGDPAGRYPIWSGEGAARQEGRWHMRGQEV
                     IYTSEHYSTAMLEKLAHFNGVLPAGQHFVAAEVPAGTTYEVVTKDSLPAWIDANAARA
                     FGAAWLGERRSAILIVPCFVAREERNVLINPAHGEARAIVPGLETPVTWDERLFAAMS
                     MRAR"
     gene            26152..>26513
                     /locus_tag="PK264_02725"
     CDS             26152..>26513
                     /locus_tag="PK264_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74843.1"
                     /translation="MGAPQLTITGVSKKFGGVTAVQDVTVEVPRGRILSVIGPNGAGK
                     TSLLNMISGFYRPDTGRIVFEGQEITPFRPSEIAALGVARTFQNIALFSGLTVLDNLM
                     LGRHVRMKSGVLSNVLYWG"
     assembly_gap    26514..26594
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26595..26933
                     /locus_tag="PK264_02730"
     CDS             <26595..26933
                     /locus_tag="PK264_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74844.1"
                     /translation="QTAALAYGLRKRVELGRALALEPRLILLDEPMGGMNQDEKEDMA
                     RYILDVNQERGVTVVLIEHDMGVVMDISDRVVVLDRGRKIAEGTPAEVQGNPAVVQAY
                     LGTKAGGGRL"
     gene            26930..28879
                     /locus_tag="PK264_02735"
     CDS             26930..28879
                     /locus_tag="PK264_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD74845.1"
                     /translation="MSRVSLVSAAETDTLPKLVRLNAKRFGDTPGIRDKDRGIWKAYS
                     WRDYQSEVRTLALGLAALGFERGHKLSVVGDNRARLYWAMLSGQALGGMAVPVYQDAI
                     ATELVYVLNHADVSVIIAEDQEQVDKVLSLKGELPALKWLIYEDPRGMTGYKQPFLKS
                     YDEVLSLGRDFDKVHPDYYDRELDLGKAADVAMIAYTSGTTGRPKGALLSHANMIAPA
                     ENYLDHERISRGDNWLSYLPMAWVGDAMFSLSTALVGGVTINCPEGPETVQRDLRELG
                     PHGILAPPRIWESLLSQMQVRSADASFIKRRAYDYFRRLAEARELLVTDGKPVPLGMR
                     LGYRLGDLLVYGPVRDHIGMRKARWCLTGGAPLGPDTFRFFRSFGINLKQIYGATEAA
                     AIVAVQKDDQADPNTVGSVIAGTEVRIGENGEVQLKGPGVFIGYYKQDEATKDTITPD
                     GWLKTGDAGIIDPRGHLAIIDRAKDVGKLADGTPVAPQFIENKLKFSPFIREAVSFGG
                     GKPFVAAMIAIDLSTAGTWAERKGLAYTSFMDLARKPEIAGLIIEEVGKINATLPEAT
                     RVKRILLLNKELEADDAEITRTRKVRRGFVAEKYAKVIEAFYGGAKEVDLETEITFED
                     GRKRTLTATIDIHDVPATASARKAA"
     gene            28892..29788
                     /locus_tag="PK264_02740"
     CDS             28892..29788
                     /locus_tag="PK264_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD74846.1"
                     /translation="MLQELEFLVSLLTSGVSIGLMYALIALGFVLVYKATDAINFAQG
                     EFVMLAGLIAATLLAAKGMPVIGAIMGAVAIMIAFSFALERVVLRPLLGRPIVAVIMA
                     TIGLAAILRGLPPITVGAETKALPLPIGDEPYKLGLISLSPIQLVGIIVCLLCLLAFS
                     WFFMKSRMGVAMRAVADNQQVAMAMGINVERYFAIAWAMAGIVSAISGVLWGNMLGVD
                     TQLALVGLKVFPVVILGGLDSVVGCIIGGVIVGVVESIAAGYLDPYVGGGTKDFAPYV
                     LMIIVLMFKPYGMFGKRKIERV"
     gene            29838..>30653
                     /locus_tag="PK264_02745"
     CDS             29838..>30653
                     /locus_tag="PK264_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD74847.1"
                     /translation="MFYRESGVYKTSYASDMALFPQPITKAAVAFWLILFAVVAPLTL
                     HDYYLSIINLVLIAIVGALGLNILVGYTGQISVGHAAFMSVGAYTAANLVVKFGLPFW
                     ITLPAGGLVAALIGAIVGIPSLRIKGLYLAIATLAGQLIIEWVINHTPAISGGAQASI
                     QVPRPTFLGMELKTQGQLYFFLLFFAVLAIVATFNIVRSRIGRAFVAIRDQDIAAEII
                     GINIYRYKLLAFAISSFYAGVCGVLYTYYLGIANYEQFQIVVSIDYLAMIIIGG"
     assembly_gap    30654..30719
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30720..30917
                     /locus_tag="PK264_02750"
     CDS             <30720..30917
                     /locus_tag="PK264_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD74848.1"
                     /translation="IVIRWIMESFGTLFFSAADLPAVISSLRLIIFGALIIVFLVLEP
                     EGLNRLWRNIRNYFRTWPFSY"
     gene            31003..32253
                     /locus_tag="PK264_02755"
     CDS             31003..32253
                     /locus_tag="PK264_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD74849.1"
                     /translation="MRMHAKRWLGAAIGVGAIATGGLMASAQTKELVFGLHCDRTGAT
                     QTVGVFLCPGYHDYIALVNKKGGVEGFKIKVVEIDNEYKVPPAVEAHERFKKDGAVLT
                     GLYGTPQTAALTKKLEEDKILGTSPGFGTAAGADGKRFPYTFPIAASYWSQGAAAVEF
                     AKKQLGGSLKGKKIAYLYYDNPAGKEPLVILDDLSKSEGFEMRTFAVPAPGVEMGAQI
                     LDITGRFKPDFVITHLFGRSPSVSIKELKGKGFPLSKVVAMVWGASEADIKAAGGFAY
                     AEGYNAMQFAGVGGDFDVIKDIVAMYKADNKPAPAAMETSVFYNRGVMVAALHVEAVR
                     NAIKAKGGAAPNGEDVKKGMEMVKGFTLGGLVPPMAITPEDHEGGGWVQIWQVKGGKF
                     VKSTEWFQGYRDVIKKHLAAEAKK"
     gene            32332..>32877
                     /locus_tag="PK264_02760"
     CDS             32332..>32877
                     /locus_tag="PK264_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74850.1"
                     /translation="MLTINNIEVVYDKVILVLKGVSLEVGEGKITTLLGANGAGKTTT
                     LKAISGVLGTERGEVTKGVISLSGAPIHGKRPFDVTKLGIAQVFEGRRVFEHLTTEEN
                     LIAGAHLIRDGKTVRDGIEMVYTYFPRLKERRGQTSGYLSGGEQQMLAIGRALMSRPK
                     VVLLDEPSLGLAPMLVEEIFGI"
     assembly_gap    32878..32938
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32939..33138
                     /locus_tag="PK264_02765"
     CDS             <32939..33138
                     /locus_tag="PK264_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74851.1"
                     /translation="NAAMALSVAEHGYVMENGRIVLEGTGDELRANSDIREFYLGLNE
                     GGAKKSYRDVKHYKRRKRWLS"
     gene            33178..>33255
                     /locus_tag="PK264_02770"
     CDS             33178..>33255
                     /locus_tag="PK264_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxy-D-gluconate 3-dehydrogenase"
                     /protein_id="HRD74852.1"
                     /translation="MIVTGAARGLGREYARALAARGAAVV"
BASE COUNT         5483 a        10654 c        10838 g         5953 t
ORIGIN      
        1 cgaagcgcag cgtgtcccgc ccgcgctcgg ccatcacctc gatcggcagg cagccgtcga
       61 aatagggcgt gccctcccac tccttgaact cggtcttctc gccggcgagg agggcgtcga
      121 cgaaggcttc gtattcgggt ttcgtcatcg ggcaattgag atagtcggcg cctgtcccgg
      181 cgggccccgc cttgtcgtag cgtgactggc gccaggcgat cgagaggtcg atgctctcga
      241 agtggacgat cggggcgatg gcatcgaaaa aggcgagatc ggcctcacct gtgagaccgc
      301 cgatcgccgc gccgagcgcc ggcgaggtga ggggcccggt cgcgacgatg acgctgtccc
      361 attcgggcgg aggaaggccg gcgatctctt cgcgcgcaat ggttaccaaa ggattcgaga
      421 cgagccgcct cgtcacctcg gccgagaatt cgtcccggtc gacggccagt gcgccgcccg
      481 cgggcagctt gtggcgatcg ccggctgcca tgatcagcga gcccgagcgg cgcagctcct
      541 cgtgcagcag gcccacggcg ttgttgtagg tgtcgtcaga ccggaacgag ttcgagcaga
      601 cgagttccgc aaggccgtcg gtcttgtggg cgttcgtacc gcgcacgggg cgcatctcgt
      661 ggagaaccac cgggacgccc ccacgcgcga gctggtgtgc cgcctcggag ccggcaaggc
      721 ccccgccgat cacgtgtacg ggcctgtcaa tgctcatagt atctgcctcg gccgcgcctt
      781 atatgctgtc tcgcaccccc aactacacca aagttgcggt caggtcaggt tcaggcgacg
      841 ggagtatggt cccgacgacg ttctcgggcc gtacagcatt ccaagggccg ccgccccgac
      901 acctcggcgg cgcccaacaa aggctgcgca atcatggcga agggcatccg ctatctgttc
      961 acacttggcg tccccgccgc cttggcgatt gctggcagtg cctacgtgct tggcatgcga
     1021 ccgcagggcg gggggccggc ctacacctat gacaccgtga ccgtcggcaa ggggcagatc
     1081 agaaagctcg tctcctcgtc aggtccggtc cgcgcgctcg tcaccgtcag catcggctcg
     1141 cagctctccg gtcagatcgc cgaactcaag gtcgacttca acgccgaggt caaggccggc
     1201 gatgtgctcg ccgtcctcga caacaagacc ttcgctgcca aagtcgctca ggcgaaagcc
     1261 gacctcgcga ctgcaaaggc gctgctcacg gcggccctcg agaaggccga ggccgtcttg
     1321 aagtccgctg agcgggttgc cgcccgccag tcgacactcg ctgcaaaggg tatcgccgca
     1381 actgtaacgg tcgacaatgc gacgcgcgac atggacgtcg ccaaggccga tatcgccgtc
     1441 gccaaggcgc agatcgagag cgcgagagcc cagatcgccc agcgcgaggc gcagctcgag
     1501 caggccatga tcgacctcga gcgcaccatc atccgctcgc cgatcgacgg gacagtcatc
     1561 gggcgcacca tcgacatcgg ccagacagtc gcggccagcc tgcaggcgcc cgagctcttt
     1621 aagatcgcgc aggacctccg ccgcatccgc atcgaggcgc aggtgaacga ggccgatgtc
     1681 ggtgcggtcg ccgaaggcaa taccgtgacg ttcacggtcg atgcctatcc cgagctgcgc
     1741 ttcaccggcc tggtgacgca ggtgcgcctt gctgcgaccg agctgcagaa cgtcgttact
     1801 tataccgtga tcatcgaggc cgccaatgcc gagcgcaagc tcttccctgg catgacggcc
     1861 aacgttcaga tcgagacggc gaagcgcgac ggcgtcgccc gcatcccgat ggatgcgctc
     1921 cgcttcaagc cgcgcaacgc cgctgctgcc tcggcaggcg ctcctgcggc cacgacgcgg
     1981 gcgagccccg aggagcgcga gcggcggatg gccgagctca ccgcgagcct caagaccgat
     2041 atcggtctca gcgaggagca gatcgccaag gtaaaggcgg agctcgcgaa gggcttccag
     2101 ggtcgccgca gtggtgcggg cgccggctcg ggcggtccct ccgctggcgg agatggtcca
     2161 ggcggcggtg ccgctcccga ccccgcacag atccgtgccc gcatgctcgc ccgcaccgcc
     2221 gaggccgttg catccgtcat cacgtccgat cagcggcccg ctttcgaggc ctggaggcgc
     2281 acgcgcgaaa cggcaaggac ggcgagcatc tggctgttgg gcgagaccgg ccaacccgtc
     2341 tcgcgccaga tccgcatcgg tatcgccgac gaccaatttg ccgaggtcct cggcgagacg
     2401 gtgaaggacg gtgagcgtgt gatcgtgcgc gcccgcgagg tgaagaagtg agtgacgcgt
     2461 ccaccagtgg cgcgccgcgc cccgcgtccg gagccccccc gctcatcggg gcgcgcggca
     2521 tttcgaaggt ctatcgcatg ggcgaccaga ccgtgcgggc gctcgacgat gtctcgctcg
     2581 aaatcaggtc cggcgagttc gtcgccatca tggggccctc cgggtcgggc aagtcgacga
     2641 tgatgaacct gatcggcgcg ctcgacgtgc cgacgtcggg tgtcctcgag atcgacgggc
     2701 gcgacattgc caagcagtcg agcgacgagc tggccgacct cagaaaccgc acaatcggct
     2761 tcgtcttcca gcagttcaac ctgctgccgc gcaccgatgc gctccgccag gtgatgctgc
     2821 cgctcgccta cgcccgcccg cgcccgacga atgccgagga gctcgctcgc caccggctcg
     2881 agcaggtgga gctcggcaat cggctcgatc atcatccgcg ccagctttcg ggcggacagc
     2941 agcagcgcgt ggcgatcgcg cgggccctcg tcaacgcgcc caagctgctc ctagcagacg
     3001 agccgacggg tgccctcgat tcgcgcacga gcgaggagat catgcgtctc ttctgccggc
     3061 tgaacgacga gggcatcaca gtcgtcctcg tcacgcacga gatggacgtc gccgcctacg
     3121 cgaagcgccg catcatgttc cgcgacggca agatcatcga ggacatagcg caaccgggcg
     3181 tcccgctcgc cgctcacccg agggccgccg aatgaacccg ctggaaagct tcaagatcgc
     3241 gctcctcgcg ctccgcacga acctattgcg ctcgatcctg acgacgctcg gcatcatcat
     3301 cggcgtcggc tcggtgatcg tgatggtcgc tgtcggcgcc ggcgcgcgga gcgaggtcga
     3361 tcgccagatc gccgcgctcg gcagccagac gctcaacgtg atcccaggct cgtcccgcgt
     3421 catgggccgc tcgggcggat cggggacgac gctgccgatc tcggaaggtg atctgaaggc
     3481 cattcgggac aagatcccgg gcgtcgtcgc catctcgggc ctgctcgggg gctcggcgca
     3541 ggtcgtgcgc ggcaacacga actggacgac ctcgatcaac ggcgtgcatg acgaattctt
     3601 cacggtacgc gaatggcagg tgctcgacgg ccgcgagttc accaaggacg agatgcgttc
     3661 cggcgcgcgc ctcgccgtga tcggccagac cgttgccttg aagctcttcg gggatggcgc
     3721 gagtccactc ggccagacca tccgcatcaa gaacgtaccg ttcacggtga tcggcgtgct
     3781 cggccccaag gggcagtcga gcttcggctc cgaccaggac gacgtcatca tgctgccgat
     3841 cacggcagcg cgcgggcgca tcgtcggacg cagccaggtc gtcaacgaca gcatcggccg
     3901 catccaggtg aagttcgacg acgacgtgaa tctcgccgag gcccaggagg cgatcgagca
     3961 gctgatgcgc cagcgccgcc gaattcagcc tggcgctgag gacgatttct tcgtgcgcaa
     4021 cttcgccgag ttcatgcgcg cgcgcacggc tgcactctcg accatgacct ggctgctggc
     4081 cgcaacttcg gccatctcgc tcatcgtcgg cggcatcggc atcatgaaca tcatgctcgt
     4141 ctcggtgacg gagcggacac gggagatcgg cttgcgcatg gcggttggcg gccggcgccg
     4201 cgacatcctc atgcagttcc tggtcgaggc ggttacgctc tgtctgctcg gcgggctgat
     4261 cggcgtgatg ctcggcggga tcgtggcttt catcgtcgca acgtctgccc agtggccgat
     4321 cctgatctcc aaggaggcga tcctggtcgc catggctgcc gcggcggcga ccggcatctt
     4381 cttcggcttc ttcccggccc ggcgcgccgc ctacctcaat ccgatcgatg cgctgcgcag
     4441 cgagtagcgg cttttcttgc cttagatcat cgatgatcgc gctcattcat gatttgttaa
     4501 ccaagctctc cggatcgtat cgcgtcggga agcaatggaa gaggagcaag tcatgtcgaa
     4561 gtctctctgg attgctggcg ctgcggcgct cctgtccgtg gccaccgttg ccggcgtgtc
     4621 ggccgctgga cctatgagcc ccatcacggg cgtcaagcct gcgagcccct cgcttgtcga
     4681 gaccgtgcat tgccggcgct actttcactg ccatcgcgtc tgccgctggg gcaggtaccg
     4741 catctggtgc tggcgcgcct gccaccgctg cggctgatgc cggagctcga tctgggctgt
     4801 gggtcgggcg agagctcgcc ccacagcttt gggggcaagg cccgaacggg ccggcacatc
     4861 agcgggaaca gtcggatcgg tcgaaagaat tgtcgccggt gtccccggta actcttgaag
     4921 tcttaacggc tctcggcata taaggcctcc atgagccagc agatccacat gatcgtcatt
     4981 cgaattatgg ccccggccgc ctgagggcgc cgggagttcc gtattttttc gatgctcgat
     5041 ctaaggatgc ctgtccgatg cgctctcagc tcgcggccgt tgccgcccca aaagtcctcg
     5101 ataacccgtc cgctcccctg gcgagggacg ctgtcgccgc ccacttcgtc gtggttgcgc
     5161 aggccgagcc cggcctgctg ccgcggctcg tcgagccggt cgccaagctc ggcttcgtgc
     5221 cgtcgcgcgt gcatgcctcg cgtgaggcgg gcgacggttc cgagatgacc gtcgatctcc
     5281 gcctcaccga cgttccggcg cgcaccgccg agcttgtcgg catggcactg cgtgcgatcg
     5341 tcggcgttca ccgtgtttcg gccgttgtcg agccgcagca ctgatcctgt gcattgttac
     5401 gtgcctgccc cagagctgcc gcaatccggc agctcgatgg gtattggtct tcatttcagg
     5461 ggagccgagc catgccgatc gcagttgccg cagccttgct cggcgcaggc ctttacgccg
     5521 ggtatcgcct ctacagctcg ctgcaggagc ggatggcgga cgaactcgcg cgcgccgaag
     5581 cagatcaccg ccgccagcga ggcgagaagg acctcggccg cctcgagttc gatccggcct
     5641 cgggcgtcta caagccgtcg cggcgggggt gagaccgtcg ccatagggcg acccgggcaa
     5701 ggtcctgctg ccccgatgtt tctttgacac cgcgccggcg cctcgatgct cagccgaatg
     5761 cggggcggca ccactttatg tgcgccggca ggagtaagca ccaattgtct tcgagcaccg
     5821 acctactaca gcctagctgc tcggcaacca ataccgtgtc cagcaggccg ttcgccagtc
     5881 atatcgcgcg gctcggaagt tgatatggcc tcgatcgcct gcctgagctg aaccgcctct
     5941 tggccgcgtt cgatttcccg actcctgacc ttgcgcctcc gacacgacgc cttgctcgtc
     6001 agtgcgcccc gctgcgtctc cgcggaattg acctttgcca agttccgcct tggccgcaga
     6061 cagccgaacc atttctgata tctgttcccg acggggttcc cgtggccgac ccgattcaca
     6121 tcgggccgaa acagccctaa gctgactgct gctgggaacc gaacggggcc ttagcttcgt
     6181 ccgttggcac gcatattttc gatcgtcagt catggtggag cgggtggcgc cacaacgctc
     6241 gccgtgcctg gcaacacacg ccgcaacatg cactgaggtc atatgcatca gcagcgagct
     6301 gtattccgca gagacattga agccttgcga gcgatttcgg tcctggcagt cgtccttttt
     6361 cacatgcgcg tgcccggatt cggtggtggt tttgcaggtg tcgacgtctt tttcgtaatt
     6421 tcggggttcc tgatttccgg ccagctgctg cgcgagcggg aaagcaccgg cggcatagat
     6481 ctattggcgt tctggtcgag gcgcgcgtgg cgattgctgc caaacgcgct actcgtactg
     6541 tcggtgacag cagtcgtcgc cgctttcatc atgtcggcgg accagaccgg accacttgcc
     6601 cttgatatcg cgtcggcgtc cgtatacatg gccaactatc ggttcgccac tagggccatc
     6661 gactatttcg acaatgcgca gcatgcaagc cccgtattgc atttctggtc gctgagcgtc
     6721 gaagagcagt tctatctgat ttggccgtgc cttctcctgc tcgcatgctg gcgtgcggca
     6781 ggcgaccgca ttcgccgtcg tgccgcgaca attgcagcgt tgatcattat tgcgagcttt
     6841 gtcgcgtcgg ccgtctacat ccatcgaaac caacccgcgg cgttcttcca tacagaggcg
     6901 cgcatctggc aacttgcagt tggagcgata ttggcggtac tggacgaccg cgcgatctta
     6961 ccggagcgac tgcggacgct cgcgagtggg gccggtctcc tgggaatctt ggggagcgtc
     7021 acgctgttga atgagaggga ttatcccggc agtgccgccc tggtgccggt cgcctcagcc
     7081 gcgctcctca ttgcagcggg ccggagtgga tcggaagccg gcgttcagcg cgtactcaac
     7141 ctagcgcccc ttcaatggct cggatcacgc tcttacagcc tgtatctgtg gcactggcca
     7201 attttgttgc tcggcaaatc cgttgaattt ggaccgatgg aagctctcgg cctcatgggt
     7261 ctttcgatag cggtcgccgc aacggcctac gctgtgatgg aagctccttt gcgccgctat
     7321 ggcatgcgcc aacaacgctc ggtccaaggg ctcgccatgc cactcgcggc gagtggctgc
     7381 atcggggcgg tcgcagtggg cctcgcggtc gtcggcatgt cctcattcaa tccattcgtg
     7441 tcggagaagg cccagcagat tgcccagctc atcgctaggg cgcgagagga tggcccacag
     7501 atcggcgcgg tcggccggtg cagggccacg atcgacaacg acccgcggtc gggctgtcgc
     7561 ttcggcgcac cgaattcgga acgaacggtc gtcctttttg gcgacagcta tgcggaacat
     7621 ttgttcgatg gaatggacgc cgctgcgcgc gaggcgggat ggtcgatgcg aatttggacg
     7681 agggccggtt gtccgcctat agatgcgccg tcattggact cgggaaaacg tgtcgtcgac
     7741 gcagattgtc tctcctggcg aaatgccgtt gtgcgccaat tgattgctga tgcacctaga
     7801 ctggtcgtcg tatcgtcgtg gtcaggcgtt gcaacaaacc ttgcggatga cagcgggcga
     7861 gcgctttctc gtggtcaatc acgccttgcc tggcgcgagg gctttgcaag cctgcttggg
     7921 cggctacgcc gtggcggtgt tgaggtcgcg gtggtgcgtg ggaccccgcg cggctccttc
     7981 tcggagctcc attcgtgcct cctcgtcggc accgccacga gcgatcgatg cgggcgcccg
     8041 cgcggcaatg ccacggccta tagctcactt gaccttgagg tggcggcaac tgtggccggt
     8101 gttcgcctgg ttgatctggc tgaccgcttc tgtgggccca acgtctgcta tagctttcgc
     8161 gatgggatga tcgtctatcg ggatcgtcac caccatttga cagcggcatt ctctcgatca
     8221 ctttcatccg atttcaagcg tctccttcag gacgtcgagt tgggacgatg aattcgcaat
     8281 tccgccggtg atgttcgtcg ccattggccc agttctacag ccagcccttc cgccggaaat
     8341 agaggaacgg cacgatcgcc gaggcgatca tcaggccgat ggcccaggga tagccgagcg
     8401 gccagctgag ttcgggcatc gccttgaaat tcatgccgta gatcgaggcg atgagcgtcg
     8461 gtggcatcaa aacgaccgag acgaccgaga agagcttgat gatctggttc tgctcgatgt
     8521 tgatcatgcc gagggtggca tcgaggagaa aggtcagccg gttcgacacg aagcgcatgt
     8581 gctccatcag cgaggccgta tcgcgctgcg ccgtctgcag ccgctcgacg aggcgcgggt
     8641 cgtcgccccg ctccttcgcc acggccagga agtaggtcag aagccgatcc atcgagaagg
     8701 cactttcctg cgctctcgag acgagatcac cctccttgcc ggtcgtcttg agcagcacat
     8761 cgagccggcg gctgcgggtc tgctgtccgc ccttgatctc gaacacgctt cgcgcgagcc
     8821 gctccgtttc gtcctggatg cgctcgacga ggtctgcctg acgagaaatg atgctctcga
     8881 tgagcccggt caggatcgtc gcacccgagc tgcactgagc atcgcccttg tcggcccgcg
     8941 cgacatagag ctgtatcgcc ttgggctccg agtagcgcac cgtcaccaga cgattgcccg
     9001 cgaggacgaa cgtcagcgtg gatgtctccg ggatcggcgc atcgacgttg tagacgatga
     9061 acgccgtcat gaaatgcgcg ccgttctcct ggtaaaacct gttcgaggcc tcgatctcgc
     9121 gcatctcggc ccgcgtcggg atcgagatgc cgagggcctt ctcgacatgc tcgtcctccg
     9181 cctccgtcgg attgaggagg tcgagccaca cggtggtccc gaggatggta tcgcaaccct
     9241 cgcgccttgc gagccctggg gtctggcctc tggtgtcgta gagtgtcagc atgcgcgcgc
     9301 gggtccgatg tcgatagcgc gcgacgctat ccagatttca gcgctaaggc cagctgaacg
     9361 cagccggatc acactcgatt tctcagccgc agtcgcatcg ccctcacccc tgacgccggg
     9421 cgcgctcgat cgcctcgacg atcatacggc gcgcatcgtc ggggccgccc cagcccttca
     9481 acttgaccca cttgccgggc tcaaggtcct tgtagtgctc gaagaagtgc tggatctgct
     9541 cgatcgtgat cagaggcagg tccttgtagc tcgttacctt ctcgtagcgc ttggtcagcg
     9601 ccggcgaggg caccgcgacg atcttctcgt ccatgccctt gtcgtcctcc atcatcagga
     9661 cgccgatcgg gcggacgttc atgacggcgc caggcacgat cgggcgcgtg ttggcgacga
     9721 cgacgtcgat cgggtcgcca tcctccgaca gggtgtgcgg tatgaaaccg tagttgccag
     9781 gatagcgcat cggcgtgtgc aagaagcggt cgacgacgag cgtgccggcg gccttgtcca
     9841 tttcgtactt gatcggctcg ccgccgatcg gcacttcgat gatcacgttc acgtcgtgcg
     9901 gcgggttgtt gccgacggca atcgcatcga tgcgcattgg gggctcctgc tgtcgtggtg
     9961 ctcaagctgt cgtggtgctc aactcgctcg tctccgaggc ccttactcga tgattaggcg
    10021 aggggtgtcc agcgcttcga gccggtcttc gcctacagtc gaagatcagg gcgcggtcct
    10081 ctctggcggc gagcggcctt gacgcgctgg tcaccgacac cgtcaaatcg aataccggac
    10141 taccggccca cctgcaaagg acgccaaccg tgacgctttc tgcgaaagcc tatgacctca
    10201 acgacgtcga ggccgtgcag gagcacttcc atgcccgcgg ctggaccgac gggctgccga
    10261 tcgtgccgcc gacgcccaag gcggtcgagg cgtgcctggc ctgggcaggg ctcgcgccgg
    10321 atcagctcat cggcatcgag cctgtgcgtg cgcagcccgt gacagcggag aagctcgcga
    10381 tcaacgcggt gatggccggc tgcctgccga tgcacttccc cgtggtcgtc gctgcgatcg
    10441 cggccatgct tcgcgaggat ttcctggttc acggcgcgac cgcgagcaca ggtggcagcg
    10501 ccgtcctcct cgtcatcaac gggccgatcc gcctcgagat tggggccagc ggcggcttca
    10561 acgcaatcgc caacagcgac cgggcgacgg ccgcgatcgg tcgcgcgctc cgcctcgtac
    10621 tgatcaacat cctcgatgtc agaccgggcg agatcgaccg ctcgacgctc ggacatccgg
    10681 gaaagttcag cttctgcatc gccgaggacg aggagggaac gggctggaca ccgctcgccg
    10741 agacgcgcgg cgtgccgaaa ggcgcctcgg cagtcacggt gatggcagcc tgtgccccgc
    10801 gccagatcat gaacgagtgg acgacgaagc cggaggagat cctggaaacg ttcgccgccg
    10861 agatgcgggc aaacctccgc cactactcga tccatcctgg caactacgcg atcgtcatcc
    10921 cgaagcagat ccgcaatcac atcgaggcgg ccggctggga caaggcgcgt atcgccacgt
    10981 tcatccacga gcgggcgcgc atcaggcgca aggaatgggc ggaggtcggc aaggcagccg
    11041 tggtgcggga ccgtggcgag agtgtctaca ccgcgctcga gcgtccagag caattgctcg
    11101 tcattgcagc gggcggccct gccggcggct ttggcgcgat cattccccca tggctcggcc
    11161 acaagagccg cgccgtaacg ctgccgatcg gcgcctgcat cgattgcgag ccgccgaaaa
    11221 aggcgtgagc caaacgcctt cggctgatgt gagctcaaat cctggaaaat cgacgccgga
    11281 attgcggagc ggcgcgctcg agattgcgcg cccggtggtg tcatacgcgt ttcgcacaag
    11341 tcttgttaaa ttcgtctccc catcgcgcag cgacttcggt ttcgctgatg cacgatcatt
    11401 acttgatccg gtcatagagc ccgatgacgc ccgccaacga aatgcccgtg cgcgctcata
    11461 tcgccatcgt cgaggatgac gatcatctgg cgctgatgct gcactacaat ctgacaacca
    11521 gagggttcgc catcacaagg ttcaaggacg ggatcgaggc gcttgcctcg ctgacgtcag
    11581 cgccgcccga tgcagttgtg ctcgattggc agcttccgcg cctctcagga attgaagtgt
    11641 tgcgccacct ccgtgccaca cctgcgactg cggaactccc catcctgatg ctgaccggac
    11701 ggacccttcc tgaagagcgg gtgcgggcga tcgccctccg cgtcgaggcg ttcatcacga
    11761 aaccgttcag cgtcagtgac gtcttggatc ggctgatgcg cgcgctggca gggcgagggg
    11821 cgcaggagcc tggtccggtg atgccatcgg cgcggcgtga gggagggatc ggatgcggct
    11881 gaacatcacc acacctagcc aatctacgat acctgatcgg gtcgcaggcg atgcatcctg
    11941 gacgaggggc gccaatccgc cacccactgc gacgattgtg atactcgccg ttggcctcgt
    12001 cgcgtcggct ctcgctctgg cgacgcttgt tgctgtcgat cgtgcaacga ggcacgcaaa
    12061 ggtcgccggc gcctgcctcg cactcgacat ggcggcagcg cacggcgtcc tcgatgaccc
    12121 gacctatcgg cgagtattgc gcgccctcag cgagggaata gacccgtttc atgaccgcct
    12181 tccgggaagt tatcgtgagc tacttgcgac ctgcgcggcg ctccgtcagg gccgttgaag
    12241 ctgcggcgtc cgggtcgcgt ggctgttcgg catctctatt ctcgcggtgg cagcatgtgt
    12301 cattcgattg aaacgtgaat cgcctacgtc taggtcatgc aatatcgacc gccaccaaga
    12361 gcgacagccg aaatccggcg ctaccgggca atcttcatct cggatatcca tctcggtacg
    12421 cgtgccgccc agtcggcagc gctgctggag ttccttcggc tctgcgacgc cgacacatat
    12481 taccttgtcg gggatatcgt cgatttctgg aaggtgcgac gcggaccata ttggccgcag
    12541 tcgcacaatg acgtcatcca gaagctcctc cgcaaggtgc gtaaaggctc gaaaatcgtt
    12601 ttcattccgg gcaatcacga cgaatgcatg cgtgactact gcggcatgca tttcggtggc
    12661 gtggatatcg agcgtgcctg catccatcgc acggccaacg ggcggcgtta tgtcgtcatg
    12721 cacggcgacg atttcgatgt tgtcgtgcgt actgcgcgct ggctcgcatt tctcggtgat
    12781 cgtggctacg aggccgcgct ctggctcaat aacccgctga actggctgcg ccgccgctgc
    12841 aaccttggct actggtccct gtcggccttt ctcaaattgc gcgtcaagcg cgccgtgagc
    12901 ttcatcgggg cctacgagga agctgtcgca acggaggcgc gccgccagca ggctgacggc
    12961 atcatctgcg gtcacatcca ccaggccgcc gaccggatga ttgatggcgt ccactacatg
    13021 aactgcggtg actgggtcga gagctgcacg gccattgtcg agacgcacga gggtcggctc
    13081 gatctcgtcc attggccgca cgacgcagca atgtccgatg acgctccaat ctccgccgag
    13141 gcgccgcccc attatgtgga agatctacga gggatcacat gacatccgag agcgtggcct
    13201 ctgacatgac cgccgcaggt ctcctatcgc gtgccgtgca tcgcaatcga gcgctgagcc
    13261 tcgacggggc gcttgagcgg cttttcacgg cggcgttcag cgatctcgtg tatccgatga
    13321 tctgggagga tccgcgcgta gatctcgcag cgctcgagat cgagccgtcg acgcgcatcg
    13381 tgacgattgc ctcggggggc tgcaacgtcc tcaactacct gacggccgat cctgccgagg
    13441 tgcatgtggt cgacctcaat ccgtcgcacg tggcgttgac gcgcctcaag ctcgcggcag
    13501 cccgtcacct cgtcgatcac gagcagttcc ttgcattgtt tgccaagggc gagggtgcgg
    13561 gcaacgccga gatcttcgac cgggtgctcg ctcacagggt cgacgctgat acccgagcct
    13621 actggacgag cgccgacggc tggcgccgtc ggaagatcga tcggttcgtc cgcggcttct
    13681 atcgccaggg gctgctcggc cgcttcatca ctttggcaca cggcgtcgct cgcctctatg
    13741 gccaagatcc tcgccggatg atgcaggccc ggtcacgcac cgagcaagtg gcgatcttcg
    13801 aggcagagct cgcgccgctg ttcgaccggc tcctggtgcg ctggatccta ggacatcggg
    13861 cggcgctcta tggccttggt attccgccgg cgcagtacgc ggccctgctc ggaggtgccg
    13921 ggcacatggc agacgtcatc cgcgagcgat tgcgcaagct cgcgtgcgat ttcgatatcg
    13981 ctgacaacta cttcgcctgg gcggccttca accgcggact cgccgcagtt ggcgaggctt
    14041 cgctgccgga ctacctcgat ccggcgaact tcgcggcgat caagtcccgc actgatcgtg
    14101 tcttggtgga gcaggcgtcc ttcacgacgt ttctctcgcg gcagccgatt tcgtccctcg
    14161 atcgctacgt tcttctcgat gcgcaagact ggatgagcga cgcagcgctc acccatctct
    14221 ggagcgagat catgcgtaca gcccgacctg gcgcccgcgt catattccgc accgccggca
    14281 cgacgacgat cctgcccggt cgcgtgccgg cgctgatcct cgatggctgg cactacgacg
    14341 cgacgcgttc tgccactctc accaaggagg accggtcggc aatttacggg gcattccacc
    14401 tctacattcg caactctcgg gcgtgagtgc atcacttgga catcgcccat cagcaggtgc
    14461 ttgacagcaa ctaccgcttc cagcgccacg tctacaacgt gacgcggcgg tattacctcc
    14521 tgggacggga tcgcctgatc agcgagttgg cggttcctcc cgaggccaac gtcctcgaaa
    14581 tcggctgcgg gacggcgcgc aatctcgtca ggctggcgga gtgctaccct gaggcccagt
    14641 tctggggcat cgatctttcg tgcgccatgc tcgcgaccgc gggcaaggcg gtcgcgcggc
    14701 gtggtctctc cggccgcgtc acccttgcgc acgccgatgc agcgtgcttc gacccacgcc
    14761 tcaccttcgg ccgcgagacg ttcgaccgga tcgtgttttc ctatacgctg tcgatgatcc
    14821 ctgactggca gctggcgatc aagcaggctg cggcctgtct cgctccatcc gggtcaattc
    14881 aaatcgtcga cttcggttcc ggccgaaggc ttccagcgac tgtccgacgt gccttgcgtg
    14941 cgtggcttcg acgcttccac gtgacgcccc gcgacgacct cgtccctttc ctcggccgcg
    15001 tcgcggaagg agggcagtac gagctcttcg cagaggagac ctaccggacc tatgccgtcc
    15061 gcgcggtcct cacgagcgca ccgagtgtgg gctgaccgct gacgttgccg gcgtatcacc
    15121 ttgcgcgcta ttgacagttg ctcctggcaa actcgggggt gccgacgtcg ctgtggacat
    15181 tcgccgcgct ggggttgcgg ccgtcgagga gccgggtcgg gaaggtcggg atcaggccga
    15241 gcgaccggag ctcgcgttcg gccccggcga ttgcctcggc cgaacgcaga tactcgacga
    15301 cgagcacggg cttgcgctcg gccttcaacc ggcggagcat gccgaggctc cacgcgatcg
    15361 atgacctggt gttgcgccgg ccagtgccag tgagcccgaa caggagatcc tccttggcga
    15421 cggcatcgat gagagtgcga taggtccggt cctcgagcag gctttctgca ttctgcgcga
    15481 tgaagaggaa atccggcttc atggcacgcg ccttccggcg aagctcgctg acgagcctga
    15541 tcatctcggt ccggctcgcg ggaaagcgcc ggcggatgtc ccagtaggca tcgacgcggt
    15601 cgaggtagat gccgtcgaaa ccggcatgga tgatctgtgc gagcaggctg tcgtcgcccc
    15661 gaaacaggtt gtgcttccac tcgtccgacc agtacttgac gaggaagttc gtctcccagc
    15721 ggcagttctc ctcgagcagc caatcgggct ttgcggcggg ccagtagtgt cgccagtaga
    15781 agcgatggtc ctcggcctcg ccgaccgaga gataggcgat cacgaggcga cggcggccac
    15841 ccggcccctt ctgcaaggcc tcgatctcgc ggcgggtgaa ggcgccctcg attgtggcat
    15901 cgcgcgacat gtcgatcacc agcatgtcgg ccggtgactg cgaaagctcc gagaggccga
    15961 gatgctggag ttggaactgc caggtgcgca cgttgcgcag ggtctctatc gcggtggtcg
    16021 cgggattggc aacagtttgt gttggtggcg ctgtcaggat cacgcatgtc gcgagcagaa
    16081 tcgacaacat cgaggtcaag gatcgcgatg ccatggcctg ccaattcctc caccaggcca
    16141 cgccactcta ttgacgtagc atgcagcgcg cgccgcaggt ttttttgctt tcgtgttcga
    16201 ttgaaatcgg ttgtcgcctg tgccgcgagc ctctgacctc tgacgcatct cggtgccctc
    16261 gggctggtgg cggacgtcag atcggcggcg gggggtagcc ttggccccag gcaatgnnnn
    16321 nnnnnnnnnn nnnccgcgct gacctttccg tacgcgagag gggagccatc gaccatggtg
    16381 accgagccgc cggccgccga gaggatcgcg tggccggcgg cgatgtccca ttcccgcgtc
    16441 ggagcaaggc gcgggtagat gtctgcctcg ccccgggcga caaggcagaa cttcaatgac
    16501 gagccggcat tgcgggtgga cgccacgcgg tagcgatcga gcatctcagc cgtttcggca
    16561 ttgagatggg agcggctcgc aacagcgacc tgcgccgcca acggtgctcg ccgggtgcgc
    16621 atcgcccgcc aggcgagacc agttacgctg ctcgccgtgt cgcccggatg gaccaaggtc
    16681 tcgcgagcgt cgcctggacc gaatgtcgcc gccagcatgc cgagcgccgg cgcatagatg
    16741 atcccgaagc gcggccggcc agcctcgatc aacgcaatgt tgaccgtgaa ttcgcgccgc
    16801 ttctcgatga actcgcgcgt gccgtcgagc ggatcgacga ggaagaacgt gtcgccggga
    16861 gttgtggcgg cacccgcgct ccaggcctcc tcggcgacaa cggggatgcc cggagcgacc
    16921 cgggcgaggg cttcgaggag gacggcctcg gcctcgcggt cggccgccgt cacgggcgtc
    16981 tcgtcggact tgagctcggt cgcgacgcct gcctcgtagt agcgcaactc gagcgcgccc
    17041 gccgccagga ctgtcggtag caggcgttcg acgaggcggt ctgaggcgag atcggacatc
    17101 ggcgactgac ctcgttcaag tggccatggc gatatggttt ggggcgacga tggacatgtc
    17161 cccggtactc gaccttgagg agcggacaac tcgtcgagcc gtctactcca ggcgcgtcga
    17221 taagcaaagc tttgtgagcg ggttgccaac cgccgtccgg gtctcgacaa aaatgcaact
    17281 cgagcgcgat gaagccgatc tcgcgcacgc ttccctctag ccggagcgcg cgacgctgtg
    17341 tatgtgacac tctgatttgc ctgcgccgcc ccgatccgtt cgctcgcgtc ccgaccccag
    17401 agcagggccc gcgcatcccg ccgagcgacg gagagccaga tgannnnnnn nnncgatctg
    17461 gaacttgccg cgctgatttc cagcaagatc tgccatgatg tgatcggacc ggtcggcgcc
    17521 atcagcaacg gtctcgagat cctgtcggac gacgacgatg cccaggcgaa gacatttgcg
    17581 ctcgacgtca tccgcaactc gacagagcag gcctcggcac gactccagtt cctgcgtttt
    17641 gccttcggcg cggcgggctc caccggcgcc atgattgatc tcggcaccgc cgagcaggtc
    17701 tcgcgcggct atgtcgggcg cgccaagcac cgcctcgcct ggaagggtcc ggccggctac
    17761 atggccaagg accaatgcaa gctgttgctc aacctgatcg ccagcggcgt tgctgccctg
    17821 ccacggggcg gcgacatcga cgtccagatc accggcgcgc tgacggcaaa tccgcgcccc
    17881 gccccctcaa aatcggtgac cgcccgccac tgttccttgc cgagttcctg gccggcggca
    17941 aggtgccgcc gctcgacgcc atgtcgatcc agccctacta cacggcctgc ctcgccgaga
    18001 ccgcacgcat gcgcgtcgcc atccgcagcg acggcgaggc aatcgtcatc gaggcacggg
    18061 tctgattcgg cacactccgg cgcttcgcac ttgatcgcgt tggtcgagct cgcgcctccg
    18121 cggctcacgt tccttgcggt cgctctcgtg gtggtggcgt ggtattgctc gtggggcata
    18181 attgcgtttg acgcgatgac cgcaaggccc cgacgatgag gattgtctcg actcgcgatg
    18241 cctggaccgg gattgcgaac tgccggacgt gtgccgtccg ccagtcggtg ctgtttgctg
    18301 gcctcgagga gagcgacttc aaccggattc accgcccgat cgagcagttc tcctacctgc
    18361 cggggacaac cgtctaccgg tccgaggagt ccgccgactg ggtctatacg atccgcgtgg
    18421 ggctggtgaa attgctgcag tacctgccgg acggcacaca gcgcatcgtc aggctgatgc
    18481 ggacgagcga tgccatcggt ctcgaagggt tggtcggcgc ccgctatgag catcacgcga
    18541 ttgcgctcca tcagaccgag gtgtgccgaa tccccctgag cgtcgttcga gagctgctcg
    18601 cgcacaagcc cgcattcagc gcggagctga tggctcgctg gcagagagcg ttgagtgaat
    18661 cggagcgcgt tatccgcgag ctcaacacgg gtaccgcgcg cggacgcgtc gtgcgtctgc
    18721 tcctctggct cgccgagagt cagggcgagg ccacgtgcga cctcttcggg cgggaagacc
    18781 tcggcgccct tctcggactc actaccgaga ccgtgagccg ctccatggcc gagttgaagc
    18841 gtcaggggca tatcatcgag ccgcgcagca atcagttcgt ctgtgacgtg gcgcggctgc
    18901 gaagcctcgt cgaataggcc gcatcgtgcg tggccttctg gacgagatta tattagcagt
    18961 tgcgaatact caatgactgg cgccggacct gggagctacc tcgcagcgaa cttgtcgaat
    19021 gcgaggcaaa catgcgctat ccatccgatc tcggaggacg ttatacgccg atgtacttcc
    19081 tggcggcact cggagccggt gggctcgccg tcagcttctt catgtacctt atgtggctga
    19141 cgccgcatcc agcctcgccc atcccgacct ttggctcgct gagctcgacc tgggccgagg
    19201 gcacgccggt catgagaggg ttgattgggt tgagtgccgc cggcatcgcg ctcttctctg
    19261 tcctgcatct gcggctcctc gcctggaacc tccgccagtg cacgcgatgg ttggcaacgc
    19321 cggccgccga ggcgctacga tcgggcaacg ccgagacgca gctcatggca ctgccgctgg
    19381 cactcgccat ggccgtcaat gtcggcttca tcttcggcgc ggtgttcgtg ccgggtctgt
    19441 gggagcagcg tgagctcctc tttccgctgg cggtcctggc gtttgcggcc atcggtatct
    19501 tggcgatgcg gatgtttctc gctttcctcg gacgcaacct cgccttcggc ggcttcgatt
    19561 gcgcgaagaa caacagcctc gggcagatgc tctcggtatt cacgtttgcg atggtcggcg
    19621 tcggcctctc ggcggcggca gccatgagtc aggtgaagtg gatcgtggcg gcggcctttg
    19681 caggcgcgat gttcttcatc gttgccgcgg gcatcctcgg cgtcgtgttc ctcgtgctcg
    19741 gcttccgcgc catgatggag catgccgcag ccgaggagac gacaccgacg ctctggatgc
    19801 tgatccctat cctcaccgtg tttggcatcg cggtctttcg gctcaagatg tcgctcgcgc
    19861 acaccttcaa ttctcctgtg acgacgggcg agattttcgc gctcctcctc gggatcgttg
    19921 cggcgcagct actgttcggc ctcatcggct gggccgtcat gtcgcgcgtg ggctacttcg
    19981 cgaagtacgt gtcgggcggg acgcgttcgc cgggttcttt cgcgcttgtc tgtcctggtg
    20041 tcggcctctt cgtctccggc cagttcctgg tccatgccgc gctcgtgagg ctcgggctga
    20101 tcgagctcta ttcgatcgct cacggactag tcctgttgcc attgctggca ctccaggtcg
    20161 cgacgatcgc gctgttcttc aagctgagca aaaagctcat gagcgcggcc ccggaggcgc
    20221 cgccgcgtgc cgctcccgcg acactcgaga cagccgcgta gacttgcacg gtcctcggca
    20281 ctacccgcgc tgccgcattg cggcgagtag gcgatgcggc agagctggag ctttcgtcac
    20341 tcgaactccc gttgccttga aaatggcgcc gtagattgcc ggcgccgtcg agatgagcgc
    20401 cggttcgccg acgcctttgg cgcctgaagg tccatgcggt gtcggggcct cgacgatgag
    20461 ggccgtgatg cgtggcatgt cgccgaaggt cgggatcagg tagtcgtgca ggttctcggt
    20521 gcgccccgga acgtactcct ccatcaacgc gaggccgaga ccttgcgcga tgccgccctg
    20581 aatctggccg atcacctgct gcgggttgat ggcgcggccg acatcgtgcg ccgctgcgat
    20641 ctcgaggacg cgggtcgtcg caagatcgag gtcgacctcg acgagggcaa cctgcgtcgc
    20701 gaaggcatag gccgaatacg gattgccctg gccgctgtga tcgagcggcg tggtcggcgg
    20761 atcgaaccgg ccgaggccgg tgaccacgtt accgatcccg ggtggcagcg cgctacgata
    20821 gtgcgcgttg gccgaggcca ccacgacctc gccattggca agcgacagct gtgatgccgg
    20881 atcggcttcg aggccatcgg gcgccccggc gaccgcgaag agacgcgcgc ggagatcacg
    20941 tcctgcgagc tcggccgcct ttcccgagac gagcgtctga cgcgaggcgc tcgtcttgcc
    21001 ggcatcgcgt gtcaacgccg tgtcgcccgt gacaagcgcg atcgtcgcga cgggaacgcc
    21061 caaggcatct gcggcgatct gcgccaggat cgtgttggag ccctggccga tgtcgaccgc
    21121 gccgcagtag agtgtgacgt ggcctgacgt gtcgacgccc atctccatct cggacggatt
    21181 ggagagcgag gtgttgccga tgccgtacca cgagcaggcg atgccgacgc cgcgccgctg
    21241 gcggcggtcg ccctgattgt aggcggtggc cgcctgcgac cagtctcgcc agtagggctc
    21301 gacggcatca atggtggcga ggagaccaac ggagctgtcg aggcgctggc cggtgattgt
    21361 aacgtcaccg gctttcagcg cattgcgacg gcggatctcg agccaatcca tcccgagacg
    21421 gtcggcgagc atgtcgagcg cggcttctga ggcgattgcg ctctgaggca caccgaagcc
    21481 gcggaacgcg cccgaaggcg gcgcgtgcgt gaaataggcc gtgccccgcg tccgcgcgtg
    21541 cggcacgacg tagggacctg gcgcatgaac gggcacgcgg ttcgcgacag tcgggcccca
    21601 gctcgcgtag gcgccggtgt cgagctccgc atggctttcg aaagcggtga tccggccctc
    21661 gtccgtggcc ccgaggcgca cacggacgcg ggcagggtgg cgcttggtgg tcgccgccat
    21721 gctctcgggg cggctgtaca cataggcgac gggtcgatgc gtcagcaagg cgccgagcgc
    21781 caccagcggg tgcacattga gatcgagctt gccaccgaat ccgccgccgc acgcagtcgg
    21841 gatcaggcgc acctgctcct tcgccaggcc gaggacgagg gcagtctcgt cgcgatgctg
    21901 gtacggcgtc tgggtcgaaa cgtggagttc gatcgtgtca ccgatgcgca ccgcataacc
    21961 ggcctcgggc tcgatgtagg cgtgttcgac gaagctcgtc tccaccgcga agtcgacgac
    22021 gtgcggggac gtgccgagtg cgcccgctac gtcgcccgag gtgacgctgc cgtcggcgag
    22081 gacgttgcca ggcttgccgg catggacgcg cggggcgtca tcggccctgg ccgcgtcgat
    22141 gccgacggtc gacggcaacg gctcgtaggc gatcggcagt tcgtgctcgc cgatcttagc
    22201 gatggccgat gcctcgccga tgagtgccag cacggcctcg ccccggaacc gcacgagacc
    22261 gtcggcgagg acgggttggt ccttgatcgt cgggtagacg ccatgcccgt tggcggggat
    22321 gtcgcgcgct gtgagaatgc gcccaatgcc ggggtggcgt gcgagggcct ggtcgaggtc
    22381 gccgatccgg aagcgcgcgt gcgcgtgcgg cgagcgaatg acgcgaacgt gaagtgcgtc
    22441 cgtgggaatg ccgtcggcgc cgtagcgctc gcggccggtg acgcgcgcct ccccgtcgac
    22501 cttggcgatg ctcgcgccga tggctccacc ggccacgggc atgagtggtt cggagaccgc
    22561 gtcctgcgtg accgcggcga tcacggcgtc gacgatcgcc tcataacccg tacagcggca
    22621 gagcacgcca ccaagcgcgt cctcgacctc gccgcgatca ggcctcggcg aacgcgctat
    22681 gagctcgcgc gcggccatga tcatgccggg cgtgcagatg ccacactggg tggcgccatg
    22741 cgcgacgaag ctctgctgca gccgcgcgcc gagcgggtcg gtcgcgagac cttcgatggt
    22801 cgtgacccgt gcacctgcga tctggccgac agggacgagg caggcgcagg ccgccgcgct
    22861 gtcgatgagg accgtgcagg cgccgcagtc gccggcattg cagccgatct tcgttccggt
    22921 aagaccgaga tcgtcgcgga gcacgtcggc gaggcggcgg agcgcaccgc cggtgacggc
    22981 gacctcgcgg ccattcacca tcaactgaat gtgtccgggc gataggccac tctcctccgt
    23041 catggcgggg ctccgagcgc cgagagtgtc cgcccgacga gaacgagcgc cgcctcacgg
    23101 cgatagtcgg cactcgcgcg gatgtcgtcg atcggcgcga gctccgcgat gtgcttggcc
    23161 gaggcgatcc gtccgaggtc gtgatcgagg ggcgcgccga cgaggtcggn nnnnnnnnnn
    23221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn ncgcggccac catggcgatc gagatgacga ggtagcgacg
    23341 cgcgccgagc ttcaggaagt gagatcgcgc cggacgctgt gactggcgga tgaggatcgc
    23401 ggtcaggatt tcgtctgggt cgagtgcggt cgtgcgcggc cccgagacga aacggctgag
    23461 cggcaggcgt cgaacatggc cgcgccgctg caattccacc tcggcatcaa gtgccaggag
    23521 cggcggaaag ccgtcggccg ccggcgaggc attgacgagg ttaccgccga tcgtgccacg
    23581 gttttggatc tggcgccctc cgacctctcg ggcggcgagc ctcaggcaag cgaaggccgg
    23641 cggcaagcgc gcgtcagcga tctcggtcca ggtggtggcg gcgccgatcc gccagagccc
    23701 gtcgcgctcc tcgatgcccc ggagctccgc gagcccggtg atgtcgaggc tggcgcgggc
    23761 gcctcgacca aagggccgca ggtcgcggcc gatccctggc acgagtgcag gatacacgtc
    23821 cgtggcgccg gcaatgatcg ccgcgccggc cgcgcgaacg ccgagtgcct ctgcgagcgt
    23881 tgccgggcgg aagtagctat cggcgccctc ggatacggcc acgtgctcca ccttcgcgct
    23941 gcgtcgtcag attggtggcg gcggggcagt cgcctgtcaa gtcaggcggc tgccctgcgg
    24001 ctctacgctc ctggcggcat gtggggtcct gtccaacgtc tcgctcatgc gcgcccttcg
    24061 caacctccgt gccgacattg gcggggtcgg ttcgcggctc gccgggacgc acaggtgaac
    24121 agccacccga cgtagctttc gcggcacaag cattgcgcct cgggtatgct gtacgatcgg
    24181 cgacgcccga gagcggaacc agcacgtcca tcgggatgga ggcacaccat gatcgcactg
    24241 ttcttcgagg tggagccgca tcccgggcat gagcaggcct atctcgacct cgccgccaag
    24301 ctgaggccgg agctcgaccg taacggcggc gtcacctttc tcaatcgcta tcggagcctt
    24361 gcccgtccgg cgcgcatgct ttcacaccag tattggcagg accgaaccca tctcgacgcc
    24421 tggcggaacc acgccggcca ccgcggcgtg caacgggcgg ggcgcgatcg gcacttcgcg
    24481 gactatcggc tgcgtgtcgg cgccgtggtc gccgagctgc ggccgggatc gccgatgcaa
    24541 tacctcgatg ggttcgaggc cgcacggacc gggcgccggt tcgtcgtcgc ggtggaggcc
    24601 aaggggagtg cggttgcgct tcatggcggc gagagcttcg agagcgtcta cgtggcgggc
    24661 gagatggtgt cgctcagctc gttcgagagc ctcggcgagg ccatcggctg catcgaagcg
    24721 gtcgttgccg ggcgcggcgt ggtcgccgca cggatcgcgg cggtcgagcg cgactatggc
    24781 ccaaggagcc gggcggaggc ccctgccgat gcaatctgaa cgggggcggc tgatcgagag
    24841 cggcttgaat tccggaccaa acgggactat gttcgtccca tatggtccaa ccaaggtggt
    24901 tccatgaccg agatcgcaag tcgcagcgcc tccgaggcaa gccggatcgc cgagttcctc
    24961 ggactgccgc gagcccgctc gatcgacgat ctcgccctcg tcgaatgcgt gcgcaagggc
    25021 ttccccgtga agacggcgag cgtggtggtg aagcgcatcg acccggatgg ccgctttctc
    25081 gacccgaccc agatcgtgcc gaaatcgacg ctgagccggc ggaccaaggc gcagcagccc
    25141 ctctcgcgcg aggagagcga gcgtattctt gcgatctcgc gggtgttcgc ggaggtgctg
    25201 cgcatctatc gcgacgatac cgggcgtagc gcccagttcc tggtgcatcc ccacccgctg
    25261 ctcggcgggg agacgccgat gcaactcgcg gttggctcga tcgcgggcgc cgaactcgtc
    25321 ttgaagctcc tcgaccgggc cgacgccggc gtcgccgtct gatggtggct cgcaagctcg
    25381 agcgggcgct ccgcgtgttc aggatcggcg atcctgccgg gcgctacccg atctggagcg
    25441 gggagggcgc cgcgcgccag gaagggcgct ggcacatgcg cggccaggag gtgatctata
    25501 cgtccgagca ctacagcacg gcgatgctcg agaagctcgc ccacttcaat ggcgtgctgc
    25561 cggcgggcca gcatttcgtg gccgcggagg ttccagccgg tacaacctat gaggttgtaa
    25621 ccaaggacag tctgcccgcg tggatcgatg ccaatgcagc gcgtgccttt ggagcggcat
    25681 ggctcggcga gcggcgttcg gccatcctga tcgtgccgtg cttcgttgcc cgcgaggagc
    25741 gcaacgtgtt gatcaatccg gcgcacggcg aagcgcgcgc aatcgtaccc ggcctcgaga
    25801 cgccggtaac gtgggacgag cggctgtttg cggcaatgtc gatgcgcgct cgatgaaccg
    25861 cgggaacagc acctcggcgc gccgcacact gtggagcttg acagttccgt gattgaatga
    25921 cgggatggag cggggtgtga agcagctctg acttggccga caacgagccc acgataatgg
    25981 ctcggcgcct gggagggact tgatgacgtt gaaccggcgc ggccggacgg ccacgtattg
    26041 tcatgaccgc cgcagctcgc gccggacggt ggcgcaatga gtgtcgaggc tcggcccggg
    26101 tcagggcagc tcgcgacggg tgcaccaact ggtgcgacaa cggctgcgcc aatgggcgcg
    26161 ccacaactca ccatcacggg cgtctcgaag aagttcggcg gcgtcacggc ggtgcaggac
    26221 gtgacggtcg aggtgccgcg cgggcgcatt ctttccgtaa ttgggccgaa cggtgccggc
    26281 aagacgtcgc tcctcaacat gatctcgggc ttctaccggc cggatacggg gcgcatcgtc
    26341 tttgaagggc aggagatcac gccgttccgg ccgagtgaga tcgcagccct cggtgtcgcg
    26401 cgcacgtttc agaacatcgc actcttcagc ggcctcaccg tgctcgacaa cctgatgctc
    26461 ggccggcacg tgcgcatgaa atccggcgtg ctcagcaacg tcctctattg gggnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnncagacg gccgcgctcg cctatggctt gagaaagcgc gtcgagctcg
    26641 gccgcgcgct cgccctcgag ccgcgcctca tcctgctcga cgagcccatg ggcggcatga
    26701 accaggacga gaaggaggac atggcgcgct acatcctgga cgtgaaccag gagcgaggcg
    26761 tgaccgtcgt cctgatcgag cacgacatgg gtgtcgtcat ggacatctcg gaccgcgtcg
    26821 tcgtgctcga tcgcggccgc aagatcgccg agggcacgcc cgccgaggtg cagggcaacc
    26881 cggccgtggt ccaagcctat ctcggcacga aggctggtgg aggtcgccta tgagccgcgt
    26941 cagtctcgtc tcggccgcag agaccgacac gctgccgaag ctcgtccggc tcaacgccaa
    27001 gcgcttcggc gatacgcccg gcatccgcga caaggatcgc ggcatctgga aagcctactc
    27061 gtggcgcgac tatcagagcg aggtgcgcac gctcgcgctc gggctcgccg cactcggctt
    27121 cgagcgcggc cacaaactct ccgtcgttgg cgacaaccgc gcccgacttt actgggcgat
    27181 gctctcgggc caggcgctcg gcggcatggc cgtgcccgtc taccaggacg cgatcgcgac
    27241 cgagctcgtc tacgtgctca atcacgccga cgtctcggtg atcatcgccg aggatcagga
    27301 gcaggtggac aaggtgctct cgctgaaggg cgagctgccg gccctcaagt ggctgatcta
    27361 cgaagacccg cgcgggatga cgggctacaa gcagcccttt ctcaagtcct atgacgaggt
    27421 gctctcgctc ggtcgcgact tcgacaaggt gcatcccgac tactacgatc gcgaactcga
    27481 tctcggcaag gctgccgacg tggcgatgat cgcctacacg tccggcacga ccgggcgccc
    27541 gaagggcgcg ctcctttcgc acgccaacat gatcgcgccg gctgagaatt atctcgatca
    27601 cgagcgcata tcacgcggcg acaactggct ctcctacctg ccgatggcct gggtgggcga
    27661 tgccatgttc tcgctctcga ccgcactcgt cggcggcgtc acgatcaact gcccggaggg
    27721 acccgagacg gtgcagcgcg acctgcgcga gctcgggccg cacggcatcc tggcgccgcc
    27781 gcgcatctgg gagagcctgc tctcgcagat gcaggtgcgc tcggccgacg catcgttcat
    27841 caagcggcga gcctatgact acttccgccg tctggcggaa gcgcgcgagc tgctcgtgac
    27901 ggacggaaag ccggtgccgc tcggcatgcg tctgggctat cggctcggag atctgctggt
    27961 ctatggtccg gtccgcgacc acatcggcat gcgcaaggcg cgctggtgtc taacgggcgg
    28021 ggcgccgctc ggcccggaca cgttccgctt cttccgctcc ttcggcatca acctgaagca
    28081 gatctatggc gccaccgaag ccgccgcgat cgtcgccgtc cagaaggacg accaggccga
    28141 cccgaacacg gtcggcagcg tgatcgccgg caccgaggtg cgcatcggcg agaacggcga
    28201 agtgcagctc aaaggcccgg gcgtgttcat cggctactac aagcaggacg aggcgacgaa
    28261 ggacacgatc acgccggacg gctggctgaa gacgggcgac gccggcatca tcgatccgcg
    28321 cggccatctc gccatcatcg atcgggcgaa ggacgtcggc aagctagcgg acggcacgcc
    28381 ggtcgccccg cagttcatcg agaacaagct gaagttcagc cccttcatcc gtgaggcggt
    28441 ctcgttcggc ggcggcaaac cgttcgtcgc ggcgatgatt gcgatcgatc tctcgacggc
    28501 tggcacctgg gccgagcgga agggcctcgc ctacacgagc ttcatggacc tcgcgcggaa
    28561 acccgagatt gcgggactca tcatcgagga ggtcggcaag atcaatgcga cacttcccga
    28621 ggcgacgcgc gtgaagcgca tcctgctcct caacaaggag ctcgaggccg acgacgccga
    28681 gatcacgcgg acgcggaaag tacggcgcgg cttcgtcgcc gagaagtacg ccaaggtgat
    28741 cgaggccttc tacggcggcg caaaggaggt cgatctcgag acggagatca ccttcgagga
    28801 tggccgcaaa cggacgctga cggcgacgat cgatatccac gacgtgccgg cgacggcatc
    28861 cgcccgcaag gccgcctgac aggagccggg aatgctgcag gagcttgaat tcctggtttc
    28921 gttgctgacg agcggtgtct cgatcggcct catgtatgcg ctgatcgcgc tcggcttcgt
    28981 gctcgtctac aaggcaacgg atgcgatcaa cttcgcgcag ggcgagttcg tgatgctcgc
    29041 gggcctcatc gcggcgacgc tgctcgcggc gaaaggcatg cctgtgatcg gcgccatcat
    29101 gggtgcggtc gccatcatga tcgccttcag ctttgcactc gaacgggtgg tgctacgccc
    29161 gctgctcggc cggccgatcg tcgcggtgat catggcgacc attggtcttg cggcgatcct
    29221 gcgtggcctg ccgccgatca cggtcggcgc cgagacgaag gcgctgccgc tgccgatcgg
    29281 ggacgagcct tacaagctcg gcctgatctc gctgtcgccg atccaactcg tcggcatcat
    29341 cgtctgcctc ctgtgcctcc ttgccttttc gtggttcttc atgaagagcc ggatgggcgt
    29401 cgccatgcgc gcggttgccg acaaccagca ggtagcgatg gccatgggca tcaatgtcga
    29461 gcgctatttc gcgatcgcct gggcgatggc cggaatcgtg tcggcgatca gcggcgtgct
    29521 ctggggcaac atgctcggcg ttgacacaca gctcgctctg gtcggcttga aggtgtttcc
    29581 cgtcgtaatc ctcggtgggc tcgactcggt cgtcggctgc atcatcggcg gcgtcatcgt
    29641 cggcgtcgtc gagagcatcg ccgccggata cctcgatcct tacgtcggcg gcggcaccaa
    29701 ggacttcgcg ccctacgtgc tcatgatcat cgtgctgatg ttcaagccct acggcatgtt
    29761 cggcaagcgc aagatcgagc gcgtgtgatc gcaggcctct gttgcagtgg acttgaatta
    29821 ggaaagcgga cggccacatg ttctaccgcg agtcaggcgt ctacaagacg agctacgcct
    29881 cggacatggc gctgttcccg cagcccatca ccaaggccgc tgtcgccttc tggctcatcc
    29941 tgttcgcggt cgtcgctcca ctcacgctgc acgactacta cctctcgatt atcaatcttg
    30001 tgctgatcgc gatcgtcggc gcgctcggcc tcaacatcct cgtcggctac acggggcaga
    30061 tatcggtcgg ccacgcggcc ttcatgtcgg tcggcgctta tacggccgcg aatctcgtcg
    30121 tgaagttcgg cctgccgttc tggatcacgc tcccggcggg cggccttgtg gcggcgctga
    30181 tcggcgcgat cgtcggaatc ccgagcctca ggatcaaggg gctctatctc gccatagcga
    30241 cgcttgcggg ccagctcatc atcgagtggg tgatcaatca cacgccggcg atctcgggtg
    30301 gcgcgcaggc ctcgatccag gtgccgcggc cgactttcct cggcatggag ctcaagacgc
    30361 agggccagct ctacttcttc cttctgttct tcgcggtgct ggcgatcgtc gcgaccttca
    30421 acatcgtgcg gagtcgcatc gggcgcgcct tcgtcgccat ccgcgaccag gacatcgcgg
    30481 ccgagatcat cggcatcaac atctaccgct acaagctgct cgccttcgcc atctcgtcgt
    30541 tctacgcggg cgtgtgcggc gtgctgtaca cctattatct cggcatcgcg aactacgagc
    30601 agttccagat cgtcgtctcg atcgactacc tcgccatgat catcatcggc gggnnnnnnn
    30661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    30721 tcgtcatccg ctggatcatg gagagctttg gcacgctgtt cttttcggcg gcggacctgc
    30781 ccgccgtaat ttcgagcctt cgcctcatca tattcggtgc cttgatcatc gtgttcctcg
    30841 tcctggagcc cgaaggtctc aatcggctgt ggcggaacat ccggaactac ttccgcacct
    30901 ggcccttttc ctactgacgt gcggctgcgc ggcggcggga acgcctgcat gactgcctga
    30961 agtgactgag aacgacgtga ctgccaaaaa tgggaggtaa cgatgagaat gcatgcaaag
    31021 cgatggctcg gcgcagcgat cggcgtcggc gcgatcgcaa ccggcggcct gatggcctcg
    31081 gcgcagacga aggagctcgt gttcgggctc cattgcgaca ggaccggcgc cacgcagacg
    31141 gtaggcgtgt tcctctgccc cggctaccac gactacatcg ccctcgtgaa caagaagggc
    31201 ggcgtcgaag gtttcaagat caaggtcgtc gagatcgaca acgagtacaa ggtgccgccg
    31261 gccgtcgagg cgcacgagcg cttcaagaag gacggcgcgg ttttgaccgg cctctacggc
    31321 acgccgcaga cggcagcgct caccaagaag ctcgaggaag acaagatcct gggcacctcg
    31381 ccaggcttcg gcacggccgc gggtgctgac ggcaagcgct tcccctacac cttcccgatc
    31441 gccgcgagct actggtcgca gggcgccgct gcggtcgagt tcgccaagaa gcagctcggc
    31501 ggcagcctca agggcaagaa gatcgcctat ctctactatg acaacccggc cggcaaggag
    31561 ccgctcgtca tcctcgatga cctctcgaag tcggaaggct tcgagatgcg cacgttcgcc
    31621 gtgcccgcgc cgggcgtcga gatgggcgcg cagatcctcg acatcacggg ccgcttcaag
    31681 ccggacttcg tcatcacgca tctcttcggg cgctcgccgt cggtctccat caaggagctg
    31741 aaggggaagg gcttcccgct gagcaaggtg gtcgcgatgg tgtggggcgc atccgaggca
    31801 gacatcaagg cagccggtgg cttcgcgtat gcggagggct acaacgccat gcagttcgcg
    31861 ggcgttggcg gcgacttcga tgttatcaag gacatcgtcg cgatgtacaa ggccgacaac
    31921 aagccagccc cggcggccat ggagacgagc gtcttctaca accgtggcgt gatggtcgcg
    31981 gcactccacg tcgaggcggt ccgcaacgct atcaaggcca agggcggcgc cgcgccgaac
    32041 ggcgaggacg tcaagaaggg catggagatg gtgaagggct tcaccctcgg cggtctcgtg
    32101 ccaccgatgg cgatcacgcc tgaagaccac gagggcggcg gctgggtgca gatctggcag
    32161 gtcaagggcg gcaagttcgt caagtcgacc gagtggttcc agggctaccg cgacgtgatc
    32221 aagaagcacc ttgcggccga ggccaagaag tgagctgatt gagcgggggc cggtccggat
    32281 ggatcggccc tcggcgttac acatgccatc agtagagccg aagcgccgcc catgctgacc
    32341 atcaacaata tcgaggtcgt ctacgacaag gtgatccttg tcctcaaagg tgtgtcgctc
    32401 gaggtcggcg agggcaagat cacgacgctg ctcggcgcca atggcgccgg caagaccaca
    32461 acactgaagg cgatctcagg cgtgctcggc acggagcggg gcgaggtgac gaagggtgtg
    32521 atctcgctct ccggcgctcc gatccacggc aagcgaccgt tcgacgtgac caagctcggc
    32581 atcgcccagg tcttcgaagg ccgacgcgtc ttcgagcatc tgacgacgga ggagaacctc
    32641 atcgccggcg cgcatctgat ccgcgacggc aagactgtcc gcgacggtat cgagatggtc
    32701 tacacctatt tcccgcgact gaaggagcgc cgcgggcaga cctcgggcta cctttccggc
    32761 ggcgagcagc agatgctggc gatcggtcgc gcgctgatga gccggccgaa ggtcgtactg
    32821 ctggacgagc cctcgctcgg cctcgcgccg atgctggtcg aggagatctt cggcatcnnn
    32881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
    32941 aacgcggcca tggcgctctc ggtcgccgag cacggctatg tgatggagaa cgggcgcatc
    33001 gtgctcgagg ggacggggga cgagctgcgc gccaactcgg acattcgcga attctatctc
    33061 ggactcaacg agggcggcgc caagaaatcc tatcgcgacg tgaagcacta caagcggcgc
    33121 aagcgctggc tgtcctaagc agggaagggg cgacatgtga gttttgctgg caaggtcgtg
    33181 atcgtcacgg gtgccgcgcg cgggctcggg cgggagtacg cgcgggcgct ggccgcgcga
    33241 ggcgccgccg tggtc
//
LOCUS       DAORNH010000014        31254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1116_length_31254_cov_2.519247, whole genome shotgun sequence.
ACCESSION   DAORNH010000014 DAORNH010000000
VERSION     DAORNH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 31254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31254
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1116_length_31254_cov_2.519247"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>358
                     /locus_tag="PK264_02775"
     CDS             100..>358
                     /locus_tag="PK264_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRD74853.1"
                     /translation="MGSYARVSTIHQNLDRQLGALRAAGCTTIFAEKASGRDVSGRPK
                     LEKAIDALGTGDVLVLAEWDRATRSMADGITIMQRVSARGAA"
     assembly_gap    359..368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <369..642
                     /locus_tag="PK264_02780"
     CDS             <369..642
                     /locus_tag="PK264_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRD74854.1"
                     /translation="DKPHLDLSTRMGQGFLAFLSALAEDERERIQKRASDGRRIAKEL
                     GVKMGRRPKLTEHQQKIARARLTEGASCRAVAADLAVHHSTISRLR"
     gene            759..1013
                     /locus_tag="PK264_02785"
     CDS             759..1013
                     /locus_tag="PK264_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74855.1"
                     /translation="MTKATSNQVDTSSPEFRARAAERRRTWTMTRYASLDAMKDAEYR
                     SWATLPDAVKFSTISEVSATAFAMKGIHVQRLPRPHRTPE"
     gene            973..1569
                     /locus_tag="PK264_02790"
     CDS             973..1569
                     /locus_tag="PK264_02790"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRD74856.1"
                     /translation="MFKDFLDLIALLNKHKAKYLVIGGYAVGYHAQARATKDLDILIL
                     PAPKNAQAVFKALQEFGAPLRTKADPQDTDRFAAQRPLSPKDFEDEDSWFMMGTPPVA
                     VDILAKIPGVVFNAAWKNRVTVTLDEQQGITAQVISRDDLIAAKLASGRPQDLLDVEA
                     IRRAQALAAQEVTAPRNTPSPPKPRKTRKKKLQAKPRP"
     gene            complement(1864..2271)
                     /locus_tag="PK264_02795"
     CDS             complement(1864..2271)
                     /locus_tag="PK264_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74857.1"
                     /translation="MSFSSCPECGETNFYEHSYSVRHFDCGFKGQWSTRTARCGGKRK
                     RMTSAPAIDDAAARYRLALLDAAQVADTIAADMAGANQASDFIDRLRQLSSQLRAFAG
                     HTAHTSFEDLTQPQAARRSGTALMAVIRRAKPW"
     gene            complement(2268..2717)
                     /locus_tag="PK264_02800"
     CDS             complement(2268..2717)
                     /locus_tag="PK264_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74858.1"
                     /translation="MRADHPKTGVKIACRFTGGAVGSIFAASHILRGVGDGKIVMAAF
                     ALAGVLLPGIGKAIKIDTLIRDYAEAAAKFKNLQAEFRRARLVWSHKPLADFEEEARK
                     LFRAMNDARKPSLTPPEWCFKLAQRKIKTGDYDHDAGGAPKAPGDVS"
     gene            2785..3000
                     /locus_tag="PK264_02805"
     CDS             2785..3000
                     /locus_tag="PK264_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74859.1"
                     /translation="MISVDTIARVRRAHFVQGKSIKAISRELRLARNIFGEIERRETS
                     EPRQRLGSGISDLIVEKVERGESGEPR"
     gene            3835..4485
                     /locus_tag="PK264_02810"
     CDS             3835..4485
                     /locus_tag="PK264_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74860.1"
                     /translation="MSTTPRALTIGITGHRPNRLAIGEARTERRLTLVLAGLRKGALA
                     RPVALSALAEGADRLFAAAALALGYRLKVLLPFKSADYETTFSDTTTTPDYRALLARA
                     AHVEELPGTLANTKAAYEAVGRATVAASDILVAVWDGRPAAARGGTPEIIEYALAAGT
                     PVVWIDAARDQLPRKLASPTAGGRRHVPLANLARRAKPITMRALGRLAEKVLEGRH"
     gene            complement(4590..>5068)
                     /locus_tag="PK264_02815"
     CDS             complement(4590..>5068)
                     /locus_tag="PK264_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRD74861.1"
                     /translation="WVADFTYIWTAEGWLYVAVVVDLYSRRVVGWSMSATMTAELVIN
                     ALVMAVWRRGRPRELLHHSDRGSQYTSEQCQRLMAEHGIQCSLSRSGNVWDNAAMESF
                     FSTLKTERTAGKIYRTRNAARADVFDYIERFYNPRRRHSTLGYLSPVAFEERQRLA"
     assembly_gap    5069..5078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5079..5302)
                     /locus_tag="PK264_02820"
                     /pseudo
     CDS             complement(5079..5302)
                     /locus_tag="PK264_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            5430..>5927
                     /locus_tag="PK264_02825"
     CDS             5430..>5927
                     /locus_tag="PK264_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRD74862.1"
                     /translation="MITVDTVGRVRRAYFVQGRKIKAIARELKLARNTVRDIVRAGSG
                     GEAPSRKYVRREQPLPQLGAFVVALDAMLTANADRPMRERLTYQRMYEELRLAGYQGG
                     YDNVRRHARAWAVREGERTTAAYVPLSFAAGEAYQFDWSHEIVVLDGITITVKVAQIR
                     LCHSRM"
     assembly_gap    5928..6065
                     /estimated_length=138
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6066..6981
                     /locus_tag="PK264_02830"
     CDS             <6066..6981
                     /locus_tag="PK264_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRD74863.1"
                     /translation="VFLGKDRKFNRRFEQLMSHHLVEPTACTPAAGWEKGQVENQVGV
                     VRKRLFAPRPSFKTLEDLNDWLTDKCVAYAKVQPHPEQTDKTVFEMFEAERPVLMAYA
                     GAFDGFHATTASVSKTCLVRFDRNKYSVQSRAVGRPIDIHAYADRIVLKQDGVVVGEH
                     ARKFGHNQTAYDPWHYVPVLARKPGALRNGAPFKDWPLPGAIEKIRRKLTGSADGDRQ
                     MVKILTAVLTDGLAAVEAACAESLDANIASVILNALARRQQAAPPPPVETPERLTLSL
                     PPIADCARYDGLRTAPATNAVEVHCGAL"
     gene            6968..7696
                     /gene="istB"
                     /locus_tag="PK264_02835"
     CDS             6968..7696
                     /gene="istB"
                     /locus_tag="PK264_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRD74864.1"
                     /translation="MERSEIFDMLGVLELAGMRAAYDEIVSVGVKRQQGVEKVIGALL
                     KAEIAAKHARSINYQMGIAKLPLAKELADLVLDGTPINAGLIEQLASGDFLQSKRNVV
                     LIGGTGTGKSHIAIAIARSVIRAGKKARFFNAVDLVNKLEAETKLGRGGRIADGITRV
                     DLLVLDELGYLPFAQSGGQLLFHLISKLYERTSIIVTTNLAFAEWAAVFGDPKMTTAL
                     LDRLTHHCDIVETGNDSWRFKNRR"
     gene            complement(7891..8244)
                     /locus_tag="PK264_02840"
     CDS             complement(7891..8244)
                     /locus_tag="PK264_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74865.1"
                     /translation="MPPQDTARPGESIRLFLVSGRPDGLITAELINWTGHTLVGGIDD
                     LAALVGRPEASRPGVYFLLGDDGDPTSPRLYIGESENVGVRLAQHERSDDKEFAECQS
                     DWNFDPGPKCNLDPG"
     gene            complement(8249..9823)
                     /locus_tag="PK264_02845"
     CDS             complement(8249..9823)
                     /locus_tag="PK264_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD74866.1"
                     /translation="MADDAVKKSRRSISDEEIALIKAMLRRGMGKTTILSYFTHPDRP
                     VNYGRISNIEQGTYGPDVVAANDAELDKYLEEWHRRGEVSSQVVAEHAVDVSSLGPVD
                     PRRLATLFDLGDDGRYILRGGETDEVECKQSFHGPGHDRLLRAVAALANNKGGYVLYG
                     VENATGVLLGLKDDRLKNTDPSQFTQAIRSAMEPCPRFEVGSVELGSAKLGAIYVHPE
                     AEAPVISTKDADSFKAGVVYFRYPGESRAISGPDFRRLLASRDKRARQEAAELARRVV
                     ELGSDAALLDLKSGQIEGRTGSLFVSPDLLQKMQFIREGEFVQKDGAAALRIVGDVSV
                     ANSPADVLVREKIVLHGITDWDVLDHFLKQERVQHPAAFVLHSCHSNKRWLPIFFYVR
                     QIGRPTEEIVELARMEETTHTAARGALLDRLGGRVSAHTRPSHASATIIKQIGDGTLP
                     RAETLTEIGKQAVAIQGWTDRGFDLEQLLQVLSALRARVKELSSDANRSSQIRKAAAW
                     LDELYFRDATQTASAG"
     assembly_gap    9925..9934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9935..11048)
                     /locus_tag="PK264_02850"
     CDS             complement(<9935..11048)
                     /locus_tag="PK264_02850"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HRD74867.1"
                     /translation="MGKTWDSVLLGQVLRPALDPHKVDASRSYPNLGIYSFGRGVFQK
                     PPIEGTNTSASTLYRVRAGQFIYSRLFAFEGAYALVPPDLCGSYVSNEFPTFDCDPQR
                     LDAGYLSWLFRRPTLWRDIAARSTGMGDRRQRIHPEQVLAEAIPLPPLDEQRRIVARI
                     DALAAKIAEARGLREGAGQEIGAVLPSAIDTIGAGDWPVVELHSVCDPERPITYGIVQ
                     AGEHIPDGVPYIRVSDMARPELTQVGMLRTAPWIAERYTRSAVITGDIVFAIRATIGK
                     MRFVPAELNGANLTQGTARIAASADADPRYLYWELQSRAAIEAIEKASKGSTFREITL
                     GRLRTIPVRLPPLHEQRRIVAELDSLPGGMGLTAWLN"
     gene            complement(11048..12601)
                     /locus_tag="PK264_02855"
     CDS             complement(11048..12601)
                     /locus_tag="PK264_02855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018496920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRD74868.1"
                     /translation="MARKPASNGATPTTAQALGSLLKSARDIMRKDKGLNGDLDRLPM
                     LTWIMFLKFLDDLEMQRESMAKAKGEKFRSAIEAPYRWRDWAARSDGITGDDLLAFIN
                     QDRDVTRPDGTSGAGLFAYLKSLTSTNGDKRRDVIATVFKGVQNRMIDGYLLRDLLNK
                     VDGIHFTASDELHTLGSLYESMLREMRDAAGDSGEFYTPRAVVRFMVKVADPQLGETV
                     LDPASGTAGFLVEAFEHLRGQVKSVADRRKLQSGSLFGCEPKPLPFLLGQMNLLLHGL
                     DAPEIDPGNSLRFKLAEIGEKERVDVILTNPPFGGEEQKGIQANFPPDRQSSETALLF
                     LQLIMTKLKRTPSLKGRHARAAVVVPNGTLFGDGVCARIKEDLLKQFNLHTIVRLPNG
                     VFAPYTSIPTNILCFDRSGPTEHVWYYEQPLPEGRRGYTKTQPMQFEEFKDCLDWWSK
                     REENERAWRVPAAELLAAGSNLDRKNPRAKVDIEHLPPEELVDSIMAKEARIAELMRE
                     IKSALKVSA"
     gene            complement(12605..14950)
                     /locus_tag="PK264_02860"
     CDS             complement(12605..14950)
                     /locus_tag="PK264_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD74869.1"
                     /translation="MVTPKLVAAGWDTDPHVLREQHPITDGRIIPAGSRYKRRAPKKA
                     DYLLSYRRDTPIAVVEAKADDKPAAEGLQQAKDYAELLGLKFAYATNGLEIVEYDYTS
                     GLETRLTAFPDPELLWRRYCAAVGITTPEAEDRVLTPFNLSLNKPPRYYQTIAINRAV
                     EAILTGRDRLLLTMATGTGKTTTAFQICWKLWSSRWNRKGEHRRPKILFLADRNFLVD
                     GPMTRDFAPLKEAIHKIGDGPVSLGREMYFGIYQALAEDERRDGLFRQFPRDFFDLVI
                     VDECHRGSARADSSWREILNYFETAVKLGLTATPRYEGSVDTYGYFGDPIYQYSLKQG
                     IEDGFLAPYRVHRVVTEWDATGWRPTQDQLDRYNRPVPDEEYLTKDFEKVVALRARTQ
                     AIARHLTEFMAKTDPMAKTIVFCVDQEHALDMAEAIGNLNAERRKTRPNYVSRVTSND
                     GATGRGQLEKFEYVELRDDSPIILTTSQLLTTGLDAPTVKNVVLARVVGSMSEFKQII
                     GRGTRVRAEYDKLWFNIIDYTGTATEKFADPNFDGDPVRVDVTNVDQPVETPEPPKPT
                     GGDEPVPGPDDPPGPLDPPGGALPRKFYVDDGEVRIVHHQVQELDPDGHVLRTIRYED
                     YAAGRVRVLCPTVFDLKVKWADPLGRTEIIQRLKDEGIEFDALQEALNQPDADPFDLL
                     CHLAFNTPLRTRRERARILKSSRKDFFDSYGPRARQILSDLLDKYADHGEAEFDIEEV
                     FAVDPLARHGTFDEIARAFGGPENLGKAVRDLQTLLYQETT"
     gene            15132..15392
                     /locus_tag="PK264_02865"
     CDS             15132..15392
                     /locus_tag="PK264_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74870.1"
                     /translation="MNLVLPQIEHLILRARSDALGLGLPAPRFPFTASMVAAVHIHKD
                     GIGDGVWFRLHDGRVFNCFAEEQDCRLTLYDDGALQRRMSAS"
     gene            15389..15571
                     /locus_tag="PK264_02870"
     CDS             15389..15571
                     /locus_tag="PK264_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74871.1"
                     /translation="MNGDEGSPICPCCMCVHSRHAPKRRVRMGDMVAVLQAVAMISGL
                     VYLVLFEVPDLIMAPA"
     gene            complement(15740..15817)
                     /locus_tag="PK264_02875"
     tRNA            complement(15740..15817)
                     /locus_tag="PK264_02875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15780..15782),aa:Pro,seq:ggg)
     gene            complement(16222..17421)
                     /locus_tag="PK264_02880"
     CDS             complement(16222..17421)
                     /locus_tag="PK264_02880"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD74872.1"
                     /translation="MSNEIIGLGMIAAMIGSIFIGFPISFTLLFLGIVFGYLGMGDLV
                     FYLMTLKFNETMMEQTLTAVPLFVFMGLMMEQANLMERLFDSIQKMFAAMRGSLFIAV
                     MFVATIFAAATGIVGASVTILGIMAGKPMIRSGYDVKLSAGLITAGGTLGILIPPSVM
                     LVVMGPVLEVPVPVLFAAAVLPGFLLAFIFTFYAMIRCWMDPSLGPPLPKELHPTSMK
                     EVWNDFFFGLVPPSALVLASLGSIFFGWATPTEGAACGALGAMLMTLAYGRMTWAKLS
                     EALIKTLEISVLILFLVAASNFFGAVFAKLGTPTMLTDYLLTLNLNRWIILIMILALI
                     FLLGWPLEWVPIVLIVVPFLLPLITKLDFNLTWFGILVAVNLQTAWLSPPVALSAYFL
                     KGVVPAW"
     gene            complement(17418..18029)
                     /locus_tag="PK264_02885"
     CDS             complement(17418..18029)
                     /locus_tag="PK264_02885"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD74873.1"
                     /translation="MADPDAGQSASPPVARGPGGLPADMPAWMSWPIRSIDTSNRFIG
                     RLVSWLVVPLMIAMVYEVVVRKTLTPTIWAYDISRMLYGALFMLGAAYGLSRGVHIRS
                     DFLYRSWPVRRQGLVDALLYIVLYFPTMIVFLWVSYAYAFKSVVEYERGMETAWMPYL
                     GPIKSCIPLAALLLVIQGISELLKSLYAARTGRWPTESGSTET"
     gene            complement(18224..19345)
                     /locus_tag="PK264_02890"
     CDS             complement(18224..19345)
                     /locus_tag="PK264_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD74874.1"
                     /translation="MKRLKWGMAGAVALGLAGAVPVAPAAAQEKSVKIRVQAVIPKTA
                     DEVRMFEVFAANVSALTRGTVTFEVLPAGAIVGVAETLDAVDKGLVEAGLAWTHFWSG
                     KHPAAMLFGSPIAGAGLGIDNFAWVSWYMNAGGKALYEQLWTEMKVNVKGIMLQPVGP
                     EALGWFKTPIKNMDDFRKMRFRTPPGIPAESYKVMGVAAVAIGGGDILPALEKGVIDA
                     AEWCCPKPDMTFGFHKVLKNYYMQGLHQVVVNADMYLNKKVWDSLSEHQKKAIEVAAD
                     ASLMQTVAYRIHENGLALKELVEKHGVILHDTPADYFTEYMKASQTLIAKYSGENAFF
                     KKVFESMKTFAGTAVPFWAQAQRANTNLLEAYAATLKKK"
     gene            19693..20316
                     /locus_tag="PK264_02895"
     CDS             19693..20316
                     /locus_tag="PK264_02895"
                     /inference="COORDINATES: protein motif:HMM:NF020498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HRD74875.1"
                     /translation="MIIDTNISCVGMSAQLIKWNIDTVGRYYRKATHPEWVITRDEAK
                     ELSKAGLKIFAIFEDYGLASKLVLTKAQGKVDGTSALEQAVRIKQPIGSTIYFAVEGL
                     PSGYKSGDLPAIRNYFAGVRDAIGGKYAIGVYGDGIVCKTLANEGICKHTWLAAASWS
                     FEGTKDFLGDWTWSIAQLGPLDIGLGKLSVDLDVANGDFGAFVVPQA"
     gene            complement(20491..21831)
                     /locus_tag="PK264_02900"
     CDS             complement(20491..21831)
                     /locus_tag="PK264_02900"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRD74876.1"
                     /translation="MSGTPSADLIISGATVVNHDGEGIRDIAVKNGRIIAIGALGALK
                     AAETVVAKGLHILPGVIDSQVHFREPGLEQKEDLATGARAAVLGGVTAVFEMPNTKPL
                     TTTAATLADKVARARNRMHCDFAFYVGGTRENIADIPHLERLEASAGIKVFMGASTGD
                     LLVEDAPSLEKIVARISRRAAFHAEDEMRLRERAGLRRKGDPTSHPEWRDPVAALMAT
                     ERLVGYAEKYRHRVHVLHVSTAEEMAYLADHKAYASVEVTPHHLTLVAPECYQRLGTF
                     AQMNPPVRDEAHRRAIWAGIANGVVDVLGSDHAPHTIEEKHHAYPDSHSGMTGVQTLL
                     PIMLDHVSAGRLSLMRLVDLTSHGPQRIFGIEGKGRIAVGYDADLSIVDLKRRETITN
                     GWIKSRAAWTPYDGVTVTGWPVGTIIRGRRVMWEGAITSPSGGAPVRFLEGALA"
     gene            21914..22777
                     /locus_tag="PK264_02905"
     CDS             21914..22777
                     /locus_tag="PK264_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HRD74877.1"
                     /translation="MAAWIRLADRGVVSVTGPEAAGFLDNLLTNDMAVLERQPALLAG
                     LLTPQGKILFEMLVVKANDGFWLETGRDAAPALAKRLSMYKLRAKVGIEDRSGDACVA
                     VALDGGTEQRAGSVRFPDPRAPGLGNRIIAVAGLAADLSDEGPYHRRRIALGIGEASR
                     DYPLGDTFPHEANWDRIGGVSFTKGCFVGQEVVARMQHKTIVRKRLVRVSGAGPLEAG
                     APVLLGEVEIGRLGTVEGRDGLALVRLDRALEAEPSLTSGGTAITIDADTLDRYRRET
                     AERDARSKAPL"
     gene            22774..23379
                     /locus_tag="PK264_02910"
     CDS             22774..23379
                     /locus_tag="PK264_02910"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRD74878.1"
                     /translation="MTEAVTRCAWGGIDDPEYTRYHDEEWGVPNSEDRALFEKLILEG
                     FQAGLSWITILRKRETFRAAFARFDPEAIARFTETDVARLMADPGIVRNRAKIEATIS
                     NARAYLDLNARTRLSTFLWDFVDGRPIINERRSMKDIPAETVESKRISKALKQHGFRF
                     VGPTTVYAFMQSMGFVNDHLLSCHRREPCARLQRAFRPTHG"
     gene            23530..24741
                     /locus_tag="PK264_02915"
     CDS             23530..24741
                     /locus_tag="PK264_02915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRD74879.1"
                     /translation="MPWLDILSSRSMTAVFTRGLSLLGLIVFLIAASAPEARAVDFPI
                     KAKQAILIDADSGAVLFQKNADDLMYPASMSKIMALAVIFKALKAGTLKLDEPILMSE
                     NAWRKGGGVSYTSAMMVPVNTREKLEQLILGIIVQSGNDASIAIAEAIAGSEEAFARM
                     MEEEARRIGLTKSTFRNSIGLFHPEHKMTARELALLARHLVIQFPEYYPWFSQREFLY
                     RKHRFFNRNPLLGLPIGVDGIKTGHIKEAGFGIVVSAKQDNRRIILVINGHERADERK
                     AEAVKILEWGFRSFGEFKLFEAGEVVGQARVWGGTRMYVPLSGLNGGAVAVVLPRFPA
                     GQKLKAELIYKGPLKPPLKKGDAVATLRVTSSSGATAETPLYAAEDVEPAGMVRRGFD
                     SMMMMAFRWMM"
     gene            24783..25404
                     /gene="tmk"
                     /locus_tag="PK264_02920"
                     /pseudo
     CDS             24783..25404
                     /gene="tmk"
                     /locus_tag="PK264_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
     gene            25404..26513
                     /locus_tag="PK264_02925"
     CDS             25404..26513
                     /locus_tag="PK264_02925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRD74880.1"
                     /translation="MARAQTIADVEALPEADRLDGAPHPRETGELVGHVGAEAELEAA
                     FRSRRMHHAWILGGPAGIGKATLAYRFARYLLAAPEERPEAGLAIPQEARTSRLVRQL
                     AHPRLAVIRRTYDAKTKRFPASIPVDEVRRLRAFLGHTAGEGEWRVVIIDTADELNVN
                     AANAVLKSLEEPPPRTLFLLVSSEPGRLLVTIRSRCRTLMLAPLSGADLRKASEGVFQ
                     DGRPIPPEDWPRLEILANGSVRRLLGLLESEGLAQHAAIMKLLTSLPVVDWTAVEQLA
                     DQLTSPQAEQRFTSFYDLLLDEIARLVRAAGGVSGSAEGLALAAKLGVPARLATWAGV
                     WETVVRSKAETLALNLDRRTLIIETISRLQSAARP"
     gene            26599..>27037
                     /locus_tag="PK264_02930"
     CDS             26599..>27037
                     /locus_tag="PK264_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRD74881.1"
                     /translation="MATSNRYYITTPIYYPNGVPHIGHGYTTIACDVLARFNRLDGRD
                     VYFLTGTDEHGLKIKQTAEGRGITPQSLVDELSARFREMCKQLHISNDDFIRTTEARH
                     IASCQELWKRCAAAGDIYLDRYAGWYSVRQEAYFDESETSVGPD"
     assembly_gap    27038..27174
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27175..28233
                     /locus_tag="PK264_02935"
     CDS             <27175..28233
                     /locus_tag="PK264_02935"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRD74882.1"
                     /translation="ERRNEVVSFVTRGLEDLSISRAKLDWGIPVPGDPSHVMYVWFDA
                     LTNYISAAGFPDEKHMLFQKFWPADLHMVGKDIVRFHTVYWPAFLMSAGVAPPRRVFA
                     HGLLLGKAGTKISKSEGAPPISEIIEEYGVDAIRYFVMREASFGQDGTYTKETLVNRI
                     NADLANNLGNLAQRSLSMINKNCGAVVPTPGAFTATDTAILAAADGMLARAREAIERQ
                     AIHLYLEAVWSVVADANRYFADEQPWAKKATDPARMGTILYVTAEVLRQVALLAQAIT
                     PAAAGTLLDLLAVPADRRNFASLGTAGRLSAGAALPEPAPVFPRYVTPVEREVAAAKA
                     AQGKGAPKGKTAKAKPQG"
     gene            28536..>29251
                     /locus_tag="PK264_02940"
     CDS             28536..>29251
                     /locus_tag="PK264_02940"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRD74883.1"
                     /translation="MPFDSRDPNGAPPSIKLVAGNSNKPLADAISQHTKIPLTKAVVK
                     RFADMEIFVEIHENVRGQDVYVLQSTSFPANDNLMEMLIVVDALKRSSARRITAVIPY
                     YGYARQDRRPGPRTPITAKLVANLIERAGVDRVMTVDLHAGQIQGFFDIPTDNLFAAP
                     VIMRDIQERFDVSNVVVVSPDTGGTVRARGLAKRIGAPIAICDKRRDKPGSAEIMNVI
                     GDVAGKRCILVDDIVDSGGTL"
     assembly_gap    29252..29361
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29362..29490
                     /locus_tag="PK264_02945"
     CDS             <29362..29490
                     /locus_tag="PK264_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HRD74884.1"
                     /translation="DSILPTEAVHAARNIRLLSIAPLLGEAILRTSREESVSSLFY"
     gene            29812..>31254
                     /gene="recQ"
                     /locus_tag="PK264_02950"
     CDS             29812..>31254
                     /gene="recQ"
                     /locus_tag="PK264_02950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRD74885.1"
                     /translation="MPSSGDGMSKALAVLETTFGYKAFRSHQAEIVETIISGGDALVL
                     MPTGGGKSLCYQVPALVRPGTAIVVSPLIALMQDQVDTLRELGVEAQFLNSTLGWREQ
                     QDVESRLLSGKLQLLYIAPERLLQERTLALLDKAKIALIAIDEAHCVSQWGHDFRPEY
                     RQLKALAERFPGVPRVALTATADARTRDEMVHELRLEGARQFVASFDRPNIRYTIAEM
                     GSLSGREQLWRFISREHPEDAGIVYCLSRKSVEETAAFLAARGRKALAYHAGLMPDAR
                     RIAQERFLKEDGLIIVATIAFGMGIDKPDVRFVGHLNLPKSVEAYYQETGRAGRDGEA
                     ANAWMAYGIQDIVTQRQWIQQSEAGDEHKKVQRQKLDALVALCEVATCRRQALLAYFG
                     ETLPEPCGNCDNCLTPPETVDATVLAQKALSAVYRTGQRYGMSYVMDVLLGKADDRIG
                     RNGHDRLSVFGIGHEEGEREWRAVFRALVAG"
BASE COUNT         5577 a         9759 c         9912 g         5591 t
ORIGIN      
        1 caatccaacg gagccgccac gcaccccaca cgccactgat cgcgttcccc cggaacgctt
       61 gatcgagatg cgctggaacg gctgatcgcg atcgccggaa tgggcagtta tgcgcgcgtt
      121 tccaccatcc accagaacct cgaccgccag ctcggcgccc ttcgggccgc cggctgcacg
      181 accatctttg ccgagaaggc atcgggccgg gacgtgagcg gcaggccgaa gctcgaaaag
      241 gccatcgatg ccctcggcac cggagacgtg ctggtgctgg ccgagtggga ccgcgcgacc
      301 cgctcgatgg cggacggcat caccatcatg cagcgcgtgt ccgcgcgcgg tgctgccann
      361 nnnnnnnncg acaagccgca cctcgacctg tcgaccagga tgggccaggg cttcctcgcc
      421 ttcctctcag ccttggccga ggatgagcgc gagcgcatcc agaagcgcgc cagcgatggc
      481 cggaggatcg ccaaggagct gggcgtgaag atggggcgcc ggccgaaact gaccgagcac
      541 caacaaaaga tcgcccgtgc ccgacttaca gaaggagcaa gttgccgcgc agtggccgct
      601 gacctggccg tgcatcactc caccatctct cggctgaggt gatgcatgcc gcactatcat
      661 ccacaggcag aagacggctt cgtctcggac acttcttcat catggcgccc tgcgtggcta
      721 cgcctgcgtt cggcagactc ccgtgctagg atgacaacat gaccaaagcg accagcaatc
      781 aagtggatac ctcgagcccc gagttccgtg cccgggccgc ggagcgccgc cggacgtgga
      841 cgatgacgcg ctatgccagc ctggatgcga tgaaggatgc tgaatatcgg agttgggcga
      901 ccctgccaga tgcggtgaag ttctccacta tttccgaggt cagcgctaca gccttcgcca
      961 tgaaaggaat ccatgttcaa agacttccta gacctcatcg cactcctgaa taaacacaag
     1021 gccaagtacc tcgtgattgg tggctacgcc gtgggctacc atgcgcaagc acgcgccacc
     1081 aaagacctcg acatcctcat cctccccgcc ccgaagaacg cacaagccgt gttcaaggct
     1141 ttgcaggaat tcggtgcgcc gctgcgcacc aaagcagacc cgcaagacac cgaccgcttt
     1201 gcagcgcagc gccccctctc gcccaaggac ttcgaggacg aagacagttg gttcatgatg
     1261 ggcacgccgc ccgtggccgt ggatatcctc gctaagattc cgggggtggt gttcaatgcg
     1321 gcgtggaaga accgcgtgac cgtcaccctc gatgagcaac agggcatcac cgcacaagtg
     1381 atttctcgcg acgacctgat tgccgcgaag ctcgcctccg gccgaccgca agacctattg
     1441 gacgttgaag ccattcggcg ggctcaagcg ctggcagcac aggaagtcac cgcaccgcgc
     1501 aacacgccgt ccccgccgaa gccccgcaag acgcgcaaaa aaaaacttca ggctaagccg
     1561 aggccgtgaa gcacggcatg tcgctctgcg cggtcgaccc accagacaga gctgcaactg
     1621 ccctataggc ctataaggat atatccatca ggcgcttacc tcgaacggaa tggcgccact
     1681 ggatcgcctg ctgcgcaccc ccgcaaccaa attttcctct gaaaatcata ggcttcgctc
     1741 ctggccggat cggagccttg attgttgata ggctcctgcg agtacatggc gagtacatgg
     1801 cgcgggagag cgtgcgcgac gcagagccgg ccgttttcct cactgacagc gaaatgtagc
     1861 gcgttaccac ggcttcgccc tccggatgac cgccatcaga gctgtcccgc tgcgacgtgc
     1921 ggcctgtggc tgagtaagat cctcgaatga cgtatgtgct gtgtggcctg cgaatgcccg
     1981 caactgcgag ctcaactgcc gcaaccgatc aatgaagtct gaagcctgat tggcgccagc
     2041 catgtcggca gcgatggtgt ccgcaacctg cgccgcatcg agaagggcca gtctgtagcg
     2101 tgcggcggcg tcatcgatcg ctggcgcaga cgtcatgcgc ttcctcttgc cgccgcatcg
     2161 cgccgtccgc gtgctccatt gccccttgaa gccgcagtcg aagtgccgga cgctgtatga
     2221 gtgctcgtag aaatttgtct cgccgcactc gggacatgac gaaaagctca tgacacgtct
     2281 cccggagctt tcggcgcgcc gccggcatca tgatcgtagt caccggtctt gatcttccgt
     2341 tgcgcaagtt tgaagcacca ctcaggcggc gtcagtgacg gcttgcgagc gtcgttcatc
     2401 gccctaaaga gcttgcgcgc ctcttcctcg aaatcggcca gtggcttatg cgaccacacc
     2461 agtcgggccc ggcggaactc ggcctggaga tttttgaact tcgccgccgc ctcggcgtag
     2521 tcgcggatca gtgtgtcgat cttgatcgcc ttgccgatgc cgggcagcag cacgccggcc
     2581 aaggcgaatg cggccatgac gatcttgccg tccccgactc cgcgcagaat gtgcgaagct
     2641 gcgaagatgc tgccgacggc gccgcctgtg aaccggcatg caattttgac ccctgttttg
     2701 gggtgatcgg cgcgcaattt tgaccccacc cttgtcgctg tgaagatttc ctcctgtgcg
     2761 tgcgtcggca cgggagcggg ggggatgatc tcggtggata cgatcgcgag ggtacggcgc
     2821 gcgcatttcg ttcaggggaa gtcgatcaag gcgatctcgc gtgagctgcg gctcgcgagg
     2881 aacattttcg gagagattga gcgacgcgag accagtgagc cgcgccagcg cctcggctcc
     2941 ggcatctccg atctcattgt cgaaaaggtc gagcgaggtg agtctggtga gccgcgctag
     3001 cgccacggct ccaacagctc cgattttatt tacggagagg ctgagtgatt tgaggcaagt
     3061 gagatgtgcc agtgcctcgg ctccagcttc gccgatatta ttgtggcata ggtcgagcgc
     3121 ggagagtttg gtgagccgcg ccagcgcttc ggctccgaca tctccaatca gattgccttt
     3181 aaggttgaga aacgtaaggt ccgcaggctg cgccaaaaat tcggccccgg cagattcgac
     3241 cctattgtcc tccaggttaa gcgaggcaag tctagtgagg cgggatagac attccgcccc
     3301 agcagcgcca atctgattga aggttaagtc gagtgatata aggctcccaa gctgatcaac
     3361 ccactcgggc agtgtcgaca ggaggttgcc cgtgaggtca agttcctcaa tgtcggggaa
     3421 ggcccagacc agcatcggcg gaagctccct caggttgttg ctatgcgagg gatcgatcca
     3481 cgaatcataa aagctataat gtcggtacct ttcacgatct gcccgcgcct ccgcgtccgg
     3541 cccaaggtac agccgcttga gccgctgcgt cctcgaaatc tcgtagattt cgtccggtac
     3601 gctggtgaga cggagaccgc cgaggtcgag tgtgtcgccg tcgccgtcga ggaagtcgcg
     3661 gatgcggcgc ctagcctcct cccgtccctc tgcctcgtct ctcaccggca tgcaacgccc
     3721 cagtcctgag tcactacaca ttccgccagc cggcgctcct ggtacgcaat cagaatatcg
     3781 cgagcccccg ccgctgtcat tgacccgcgc cggccctgcg ataggctccg catcatgagc
     3841 accacaccgc gcgccctcac catcggcatc acgggccacc ggccgaacag gctggcaatc
     3901 ggcgaggcgc gaacggagcg gcgcctcacg ctggtgctcg ccggattgcg caagggagca
     3961 ctcgctcgcc ccgttgccct ctccgcgctg gcggaaggcg ccgaccggct gtttgccgcg
     4021 gcggcccttg cgctcggcta ccggctcaag gtgctcctgc ccttcaagag tgccgactac
     4081 gagacgacat tctccgacac cacgactacg ccggattatc gagcactgct cgcacgcgcg
     4141 gcgcacgtcg aggaactgcc gggaacactt gccaatacga aggcggccta cgaggcagtt
     4201 ggccgagcaa cggtcgcggc ctcagacatt ctcgtcgcgg tctgggatgg caggccagcg
     4261 gcggctcgcg gtggcacgcc cgagatcatc gagtatgcgc tcgcggcggg gacgcctgtc
     4321 gtgtggatcg acgccgcgcg tgatcagttg ccgcgaaagc tcgcatcccc gacagccggc
     4381 ggccgccgac atgtgccatt ggccaatctt gctcgccggg cgaagccgat taccatgcgg
     4441 gcgctcgggc ggttagcgga gaaggtactc gagggacggc actgaacacg acacgtgtcg
     4501 tgacgattgc aacttgcgcc ggtagcaatc agagttctcc cgactaccct tgcgcagtat
     4561 gagctgctgc cggttctgtg gacggtgagt taagctaatc gctgtcgttc ctcgaaggca
     4621 accgggctca gatagccgag cgtcgagtgc ctgcgtcggg gattgtagaa ccgctcgata
     4681 taatcgaaca cgtcagccct cgcggcattc cttgtccggt agatcttccc cgccgtccgc
     4741 tcggtcttga gcgtcgagaa gaagctctcc atcgcggcgt tgtcccacac gttacccgag
     4801 cgcgacagcg agcattggat accatgctcg gccatcagcc gctggcactg ctcgctggtg
     4861 tattggctgc cgcgatcgga gtgatgcagc agctcgcgcg gccgacctct tctccagacg
     4921 gccatcacga gcgcattgat aaccagctct gcggtcatcg tcgcgctcat cgaccacccg
     4981 accacgcgac gggagtagag gtcgaccact acagcgacgt agagccagcc ttcggctgtc
     5041 cagatgtagg tgaagtcggc cacccattnn nnnnnnnngc atcgaactgt cgatcgagca
     5101 cgttggccgc cagctcgttg gcggggcgct cgccagtgtc aacgggcaga cggcggcgtc
     5161 tcggcctggc ccgcagcgca ttgagacgca tcagccgctc gacggcatgc aggccgcagg
     5221 caacgcccgc ttccagaagg tcgtgccaga cgcgtcgcgc gccataggtc cggtcactgg
     5281 agacaaagct gtcgcggatt gcgagcagca tgtgaaccgg cgtgcaattt tgacccctct
     5341 acgggggtga tcgaactgca attttgaccc cactcccgtt cggtgcagat gacctgctgt
     5401 gcgtgcgtcg cgcagaggtc tggtcgggga tgatcactgt ggacacggtt ggcagggttc
     5461 ggcgggcgta tttcgtccag ggccggaaga tcaaggcgat cgcgcgcgag ctgaagctgg
     5521 cgcggaacac tgttcgcgac attgtgcgtg cgggttccgg cggtgaggcg ccctcgcgca
     5581 agtacgttcg ccgcgagcag cctctccctc agctgggcgc gttcgttgtg gcgctggacg
     5641 cgatgctgac ggcgaatgcg gaccggccga tgcgtgagcg tctgacctat cagcggatgt
     5701 acgaggagtt gcgtctggcc ggctaccagg gcggttacga caacgttcgg cgtcacgcgc
     5761 gggcatgggc ggtgcgcgaa ggcgagcgga ctacggcggc ctatgtgccg ctgagctttg
     5821 cggcgggcga agcctaccag tttgactgga gccacgagat cgtcgtgctc gacggcatca
     5881 cgatcacggt gaaggtggcg cagatccgcc tatgccacag ccgtatgnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnncgtgt tccttggcaa ggaccgcaag ttcaatcgac gcttcgagca gctgatgagc
     6121 catcatctgg tcgagccgac ggcgtgtacg ccggcagccg gctgggagaa ggggcaggtc
     6181 gagaaccagg ttggtgttgt gcggaagcga ttgtttgcac cgcggccgtc gttcaagact
     6241 ctggaagatc tgaacgactg gctcaccgac aagtgcgtgg cttatgccaa ggtgcaaccg
     6301 catcccgaac agacggacaa gacggtgttc gagatgttcg aagccgagcg cccggttctg
     6361 atggcgtacg ccggcgcgtt cgacggcttc catgcgacaa ccgcgtcagt gtcgaagacg
     6421 tgcctcgtgc gctttgatcg caacaagtac tcggtgcagt cgcgcgcggt cggccgcccc
     6481 attgacatcc atgcctacgc cgatcggatc gtgctgaagc aggacggcgt tgtggtcggc
     6541 gaacacgcgc gcaagttcgg ccataaccag acggcttatg atccctggca ctatgttccg
     6601 gtgctggcgc gcaagccggg agcgctcagg aatggcgcgc cgttcaagga ctggccactg
     6661 cccggtgcga tcgagaagat ccggcgcaag ctgaccggga gtgctgacgg cgaccgtcag
     6721 atggtcaaga tcctgaccgc cgtgctgacc gacggcctgg cggcggtcga ggccgcttgc
     6781 gcggaatcgc tcgacgccaa catcgccagc gtgatcctga atgcgctggc ccgacgacag
     6841 caagctgctc cgccgccacc ggtcgagacg ccggagcggc tgacgctcag cctgccgccg
     6901 atcgccgact gcgcccgcta cgatgggctg cgaacagccc ccgcaacgaa cgctgtggag
     6961 gtccactgtg gagcgctctg agatcttcga catgctgggc gtgttggaac ttgctggcat
     7021 gcgggctgcc tatgacgaga tcgtatctgt cggtgtcaaa cgtcagcagg gcgtcgagaa
     7081 ggtgatcggc gcgctgctca aggccgagat cgcggcgaag catgctcgct cgatcaacta
     7141 tcagatgggc atcgccaagc tgccgctggc caaggagctg gccgatctgg tgctcgacgg
     7201 cacaccgatc aacgctggct tgatcgaaca gctcgcctcc ggcgacttcc tgcaatccaa
     7261 gcgcaacgtc gtgctgatcg gcggcaccgg aacaggcaag agccacattg cgatcgccat
     7321 tgcgcgcagc gtgatcaggg ccggcaagaa ggcgcgcttc ttcaacgccg tcgatctggt
     7381 caacaaactc gaggccgaaa ccaagctcgg tcgcggcggc cgcatcgccg atggcatcac
     7441 ccgcgtcgat ctgctggtgc tcgacgagct tggctatcta ccgttcgcac aatcgggcgg
     7501 acagctgctg ttccacctga tcagcaaact ctacgaacgc acgtcgatca tcgtgacgac
     7561 gaacctcgcc ttcgccgaat gggccgctgt gttcggtgat ccgaagatga cgaccgcgct
     7621 gctcgatcgc ctcacgcacc attgcgacat cgtcgagacc ggcaacgaca gctggcgctt
     7681 caagaaccgc cgctgattac agcacgcggc gccccaacgt tcggcgctga gcatgccaac
     7741 gtgctcgcca ccctcaaggc gcggcctgga agcggctgtc ccagcatcga gacctgcgtg
     7801 ccggccgacc ttgacggcgt ctgcgcgcgt cagcgaaacg cgctgccaag agttgggaca
     7861 caccctggac gccacccgca aaacccgcat ctacccgggg tcaagattgc acttcggtcc
     7921 ggggtcaaag ttccaatccg attgacactc ggcaaactcc ttgtcatcgc tgcgctcatg
     7981 ctgggccaag cgcacaccga cgttctcgct ctcgccaatg tacaatctag gactcgtcgg
     8041 atccccgtcg tcgccgagca gaaaatagac gccgggtcgt gatgcctcgg ggcggccgac
     8101 gagtgcggca agatcatcaa taccgccgac aagcgtgtgg cccgtccaat tgatcagttc
     8161 tgcagttatc agtccatcgg gacgaccgga aaccagaaag aggcggatgg actcgccggg
     8221 ccgagctgtg tcttgcggtg gcatgctttc atcccgcgct tgcggtttgt gtggcatcgc
     8281 gaaaatatag ctcgtccagc caagccgctg cttttcgaat ttgagaagac cgattggcgt
     8341 cggacgatag ctccttcact cttgccctaa gggctgagag aacctgaaga agctgctcca
     8401 gatcgaagcc acgatcggtc catccttgga ttgccaccgc ctgctttcca atttcggtca
     8461 gcgtctcggc tcgtggcaac gtgccatccc caatttgctt gatgatcgtg gccgatgcat
     8521 gtgacggcct cgtatgggcg gatacgcgcc ctccaagtcg atctagcagc gcgccgcgcg
     8581 cagctgtgtg cgtcgtttcc tccatcctcg ccaactcgac gatttcctcg gtagggcgac
     8641 caatctggcg aacgtaaaag aaaattggca gccacctctt gttcgaatgg catgagtgca
     8701 gcacgaatgc agccggatgc tgaacgcgtt cctgcttcaa aaaatgatcc aagacgtccc
     8761 aatctgtgat gccgtggaga acgatcttct cgcgaacgag gacatcagca ggtgaattcg
     8821 ccactgagac atcgcccaca atgcggagcg ccgcagctcc gtccttctgc acaaactcgc
     8881 cctcacgaat gaactgcatc ttctgcaaca agtcggggct gacgaacagc gaccctgttc
     8941 ggccctcaat ctggccgctc ttcagatcga gcagggctgc gtcgcttccc agctcaacga
     9001 cgcgccgagc gagttccgca gcttcctggc gggctctttt gtcgcgagac gcgaggaggc
     9061 gccgaaaatc cggtccggaa attgcgcgac tctcgccggg ataacgaaag tagacgaccc
     9121 cggccttgaa gctgtccgca tccttggtcg agatgacggg ggcttctgcc tcgggatgga
     9181 cgtagattgc accgagcttc gcgctaccga gttcgacgga accgacctcg aatcgtgggc
     9241 acggctccat ggcgctgcgg attgcttgtg tgaactggct cgggtccgta ttctttagcc
     9301 tatcgtcctt tagacccaac aagacgccag tcgcgttctc gacaccgtac aacacgtagc
     9361 cgcccttgtt gttggcgagt gccgccacgg cgcgaagaag acgatcatga cccggtccgt
     9421 gaaagctctg cttgcactcg acctcgtctg tctcgcctcc acggagtatg taacggccat
     9481 cgtccccgag atcaaaaagc gtagccaacc gtctgggatc gacaggccca agggacgata
     9541 catccaccgc atgttcagct acgacctgag atgagacctc gccccgtcgg tgccactcct
     9601 ccagatattt gtccaactcg gcatcgttcg ccgccacaac atctgggcca taggtgccct
     9661 gctcgatgtt ggaaatgcgc ccgtagttca caggccggtc agggtgcgtg aaatatgaca
     9721 ggatcgtcgt tttgcccatg cctcggcgca gcatggcctt gatcagcgcg atctcttcat
     9781 cgcttatcga acggcgagat ttcttgacgg catcgtctgc catcacagct cccccttgaa
     9841 tgcgcggtcg agaatggcgg ggaggagggc gtcgagttcg gcggcggttt ctgaagtcat
     9901 tgcaacacct tcatggcgtg cgcgnnnnnn nnnnggttca gccacgccgt caaacccatg
     9961 ccgcccggca gcgagtccag ctcggcgaca atgcggcgtt gctcgtgaag gggtggtagt
    10021 cgcacaggaa ttgtccgtag ccgaccaagt gtgatctctc gaaacgtcga ccccttgctc
    10081 gctttctcga ttgcttcgat cgctgcgcgg ctctgcagct cccaatacag gtatctcgga
    10141 tcggcgtcgg cggaagctgc gatccgggcg gttccttgcg tcaggtttgc tccgttcagc
    10201 tctgccggga cgaaccgcat ttttccaatc gtcgctcgga tggcaaacac tatgtcgccg
    10261 gttatgaccg cagacctcgt ataccgctcg gcgatccacg gggctgtgcg caacatccca
    10321 acttgcgtaa gctcgggcct cgccatgtcg ctcactctga tatacggcac gccgtctggg
    10381 atatgctccc ctgcctgaac gatcccatat gtgatcggcc gttctggatc gcagacggag
    10441 tgaagctcga cgactggcca atctccagca ccgatcgtgt cgattgctga agggagcacc
    10501 gctcctatct cctgcccagc cccttcccgc aacccgcgcg cctcggcgat cttggcggcg
    10561 agggcgtcta tgcgtgcgac gatgcggcgc tgctcgtcga gcggcgggag cgggatagcc
    10621 tcagccaaca cctgctcggg atggatgcgc tgacggcgat cgcccatgcc cgtgctccga
    10681 gcggcgatgt cgcgccatag cgtcggccgc cggaacagcc aggacagata gccggcgtcg
    10741 aggcgctgcg gatcgcagtc gaacgtcggg aattcattcg agacgtaact gccgcacagg
    10801 tcgggtggca ccagcgcgta ggcaccctcg aacgcgaaca ggcgactgta gatgaactgg
    10861 ccggcgcgca cgcggtagag tgtcgaggcg ctcgtgttcg tgccctcgat cggcggcttt
    10921 tggaacacgc cgcggccgaa gctgtagatg ccaaggttcg gatagctgcg ggacgcgtcg
    10981 accttgtgcg ggtcaagcgc cgggcgcagc acctggccga gcagaacgct gtcccaggtc
    11041 tttcccatca ggcgctcacc ttcagcgccg acttgatctc gcgcatcagc tcggcaattc
    11101 gcgcttcctt tgccataatg ctgtcgacca gctcttccgg cggcaagtgc tctatatcca
    11161 ccttggcgcg cgggttcttg cgatcgaggt tgctgccggc cgccagcaac tcggccgcgg
    11221 gcaccctcca cgcccgctcg ttctcctcgc gcttgctcca ccagtcgagg cagtccttga
    11281 actcctcgaa ttgcatcggc tgggtcttgg tgtaaccgcg ccgtccctcg ggcagcggct
    11341 gctcgtagta ccagacgtgc tccgtcggcc cggagcggtc gaagcacagg atgttggtcg
    11401 ggatcgacgt atagggcgca aatacgccgt tcggcagccg cacgatcgtg tgcagattaa
    11461 actgcttgag caggtcctcc ttgatccgcg cgcatacgcc atcaccaaac agcgtgccgt
    11521 tcggtacaac gacggcggcg cgcgcgtgcc gccccttgag gctcggcgtc cgcttcagct
    11581 tcgtcatgat caactgcaag aacagcagcg ccgtttccga gctttgccgg tcgggcggga
    11641 aattggcctg gatgcctttc tgctcctcgc cgccgaacgg gggattggta aggatgacgt
    11701 cgacgcgttc cttctcgccg atctcggcga gcttgaagcg cagcgaattt ccgggatcga
    11761 tctccggcgc gtcaagcccg tgcaacagca ggttcatctg accgagcagg aatggcagcg
    11821 gtttcggctc gcaaccaaac agagagcccg attgcagctt gcgccggtcg gcgacgctct
    11881 tcacctggcc gcgcagatgc tcaaacgctt ccacgaggaa gcctgccgtc cccgaggcgg
    11941 ggtcgagaac cgtttcaccg agctgagggt ccgcaacctt gaccataaaa cgcacaacgg
    12001 cgcgcggggt gtagaactcg ccggagtcgc cggccgcatc ccgcatttcc cgtaacatcg
    12061 actcatagag cgagccgagt gtgtgcagct cgtcgcttgc ggtgaagtgg atgccgtcca
    12121 ccttgttcag aagatcgcgc agcaaatagc cgtcgatcat ccggttctgc acgcccttga
    12181 acacggtcgc gataacgtcg cgacgcttat cgccattggt cgatgtcaag ctcttgagat
    12241 acgcaaagag gccggcgccg cttgtgccgt cggggcgggt gacgtcgcga tcctgattga
    12301 tgaaggctag cagatcgtcg ccggtgatgc cgtcggatct cgcggcccag tcgcgccaac
    12361 ggtagggcgc ctcgatggcg gagcggaact tctcgccctt cgccttggcc atgctctcgc
    12421 gctgcatctc gaggtcgtcg agaaacttta gaaacatgat ccaggtgagc attggcagac
    12481 ggtcgaggtc gccgttgagc cccttgtcct tgcgcatgat gtcacgcgcg gatttcagga
    12541 ggctgcccag ggcctgcgcc gtggtcggcg tcgcaccgtt gctcgcgggt ttgcgggcca
    12601 ttcgctaggt ggtctcctga taaagcaggg tctgcaagtc acggacggcc ttcccgagat
    12661 tctcggggcc accgaacgcg cgtgcgattt cgtcgaaggt gccgtgacgc gccaggggat
    12721 cgacagcgaa cacttcctcg atgtcgaatt ccgcctcgcc gtggtcggcg tacttgtcga
    12781 gcaggtcgga caggatttga cgcgccctcg gtccgtagct gtcgaaaaag tccttacggc
    12841 tcgacttcag aatgcgggcg cgctcgcgac gcgtgcgcaa tggtgtgttg aaagccagat
    12901 gacagagcag gtcgaatggg tcggcgtccg gctggttcaa cgcttcttgc aaggcatcga
    12961 actcgatgcc ttcgtctttg agccgttgaa taatttccgt ccggccgagc ggatcggccc
    13021 atttgacctt caagtcgaac accgtcgggc aaagtacccg cacgcgaccg gcggcatagt
    13081 cctcgtagcg gatcgtgcgt agcacgtggc catcggggtc gagctcttgg acctgatggt
    13141 ggacaatccg cacctcgccg tcgtcgacgt agaacttgcg cggaagggca ccgcccggcg
    13201 gatcgagcgg tccaggggga tcatcgggac cgggtaccgg ctcatcccca cccgtcggct
    13261 tgggaggctc cggtgtctcg acgggctggt ccacgttcgt gacgtcgacg cgtacgggat
    13321 cgccgtcgaa atttggatcg gcgaatttct cggtcgccgt gcccgtatag tcgatgatgt
    13381 tgaaccagag cttgtcgtac tcggcgcgga cgcgcgttcc acggccgatg atctgcttga
    13441 actcgctcat cgagccgacg acacgggcca acaccacgtt cttcacggtc ggggcatcga
    13501 ggccagtcgt gagaagctga gaggtcgtca ggatgatcgg cgagtcgtct cgaagctcga
    13561 cgtactcgaa cttttccaat tgccctcgcc ctgtggcgcc gtcgttcgag gtgacccgac
    13621 tgacgtagtt cggccgcgtc ttgcgccgct cggcattgag gttgccgatg gcctcggcca
    13681 tgtcgagcgc gtgctcctga tcgacacaga acacgatggt cttggccatt ggatcggtct
    13741 tggccatgaa ctcggtcagg tggcgcgcga tggcctgcgt gcgggcacgc agcgcgacga
    13801 ccttctcgaa gtccttggtc aggtattctt cgtccgggac gggccgattg tagcggtcga
    13861 gctgatcctg cgtcggccgc catcccgtag cgtcccattc ggtcaccacg cgatggacac
    13921 ggtaaggcgc gaggaacccg tcctcgatgc cttgcttcag gctgtactga taaatcggat
    13981 cgccgaaata tccgtaggtg tcgacgctgc cctcgtaacg cggtgtggcg gtcaggccga
    14041 gcttcacagc cgtctcgaag tagttcagga tttcgcgcca agagctgtcg gcacgggcgc
    14101 taccgcgatg gcactcgtca acgatcacga gatcgaagaa gtcgcgcgga aactggcgga
    14161 atagcccatc gcgacgctcg tcctcggcga gagcctgata gatgccgaaa tacatttccc
    14221 gaccgagcga gactgggcca tcaccgatct tgtgaatggc ctctttcaat ggtgcgaagt
    14281 cccgtgtcat cgggccatca acgagaaaat tgcggtcggc caggaacagg atcttcgggc
    14341 ggcgatgctc tccctttcgg ttccagcggg aagaccagag cttccaacag atttgaaagg
    14401 ccgtcgtcgt cttgcctgtg ccggtcgcca ttgtcagcag caggcgatcg cggccggtca
    14461 ggatggcttc gaccgcccga ttgatagcga tagtctggta gtagcgaggg ggcttgttca
    14521 gggacaggtt gaagggcgta agaacgcgat cctcagcctc cggagtcgtg atgccgacgg
    14581 cagcgcaata gcggcgccac agaagctccg gatccgggaa ggcggtcagc cgtgtttcga
    14641 ggccactcgt gtagtcgtac tcgacgattt cgaggccatt cgtggcgtag gcgaacttga
    14701 gtcccagcaa ttcggcatag tccttagcct gctgcaaacc ttcggccgct ggtttatcgt
    14761 cagccttggc ttcgacgacc gcgattggcg tatcgcggcg atagctcagg aggtagtcgg
    14821 ccttcttggg cgcgcgtcgt ttgtagcggc tcccggccgg tatgatccgc ccgtcggtga
    14881 tgggatgctg ctcgcggagc acgtgcgggt ccgtatccca gccagctgca accagcttgg
    14941 gagtgaccaa tctgcggcat gtatcggctt cgttagccac ccctccgacc tacccgtcag
    15001 cgcctcgatg gagcttaggt aactcaacca gttaggggag tcgacggaac aagccgaagt
    15061 gatcaacggt taagtgcgtc atccttttgg cagcgcttgc attctctcga ttgaagcgca
    15121 taccgattat aatgaatctt gtgcttccgc agatagaaca tttgatactg cgcgctcggt
    15181 cggatgcgct cggactcggc ttgcctgctc cgcgatttcc gttcacggca tcgatggtcg
    15241 cagccgtaca tatccacaaa gatggcatag gcgacggtgt ctggttccgg ctgcatgatg
    15301 gccgcgtgtt caattgcttc gccgaggagc aggactgccg gctcaccctg tatgacgatg
    15361 gcgcacttca gcgccgaatg agtgcgtcgt gaacggcgac gagggcagtc cgatctgtcc
    15421 ctgctgcatg tgcgtccaca gtcgccacgc gccgaagcgg cgagtgcgca tgggcgacat
    15481 ggtcgctgtc ctgcaggctg tcgcgatgat ctctgggctc gtctacctgg tgcttttcga
    15541 agtgcctgac ctgatcatgg cgccggcata atcgcggctg gcggtggctc aaggcattgt
    15601 tcgcgccggt ggccggttgt cgcagatggc acgacaaaag ctgtcaggaa ccccaagaat
    15661 ccagctctgt catggtcgct aattggtcgc tagctgtttt tcagggttgg tcgctaggtt
    15721 cataactgct tgatttaatt ggtcggagcg gcgggatttg aacccacgac ccccagtccc
    15781 ccagactggt gcgctaacca ggctgcgcta cgctccgctt cttggtacgg acaagcgctt
    15841 ggcttgccga ttcctttggt ccttccggtg cttactgcac tcgggccgca ctcgggggtg
    15901 agtgcggcag acgcccggcg tcagccggtc agcgcatcgt ctaacagatg ccgaggcagc
    15961 gaggcaaaca aaattggcca ggccggtgcc tcatagtggt gcatcgtggt gtctggcggc
    16021 ggcgacggag gcgggtgcac ctcccccgtc gcggccggaa gcctgcgcgg tcgccatctc
    16081 gacgggtcgg cgcgcctact tcacctcgcc gtaccagagc ctcggcagcc agagggcgat
    16141 ctcggggaag atcatgatga ggaacagccc gatgagctgg agaaccatga actgcaccat
    16201 gcccttgtag atgtcggtga gctaccaggc cggcacgacg ccttttagga agtatgctga
    16261 gagcgccaca ggcggcgaca gccacgccgt ttgcaggttc acggcgacca ggatgccgaa
    16321 ccaggtgaga ttgaagtcga gcttcgtgat gagcggcagc aggaacggca cgacgatcag
    16381 cacgatcggc acccactcga gcggccagcc gagcaggaag atcagcgcca ggatcatgat
    16441 caggatgatc cagcggttga ggttcagcgt cagcaggtaa tcggtcagca tggtcggcgt
    16501 gccgagcttg gcgaagacag cgccgaagaa attggaggcg gccaccagga acaggatcag
    16561 gacggagatt tccagcgtct tgatgagtgc ctcggaaagc ttggcccagg tcatgcggcc
    16621 gtaggcgagc gtcatgagca tggcaccgag cgcgccgcag gcggcacctt cggttggcgt
    16681 cgcccagccg aagaagatcg agccaagcga ggcgaggacg agggcagacg gcggcacgag
    16741 gccgaagaag aagtcgttcc aaacttcctt catgctggtc ggatgcagct ccttcggcag
    16801 cggggggccg agcgagggat ccatccagca gcggatcatg gcgtagaatg tgaagatgaa
    16861 ggcgaggagg aagcccggca gcaccgcggc tgcaaacagc accggcaccg gcacctcgag
    16921 caccggcccc atgacgacga gcatgaccga gggcgggatc aggatgccga gcgtgccgcc
    16981 ggcggtgatg agaccggcgg agagtttgac gtcgtagccc gagcggatca tgggcttgcc
    17041 ggccatgatg ccgaggatgg tcactgaggc gccaacgatg ccggtcgcgg cggcgaagat
    17101 cgtggcaacg aacatgacgg cgatgaacag cgagccgcgc atggcggcga acatcttctg
    17161 gatgctgtcg aacaggcgct ccatgaggtt cgcctgctcc atcatcagtc ccatgaagac
    17221 gaagagcggg actgcggtca gcgtctgctc catcatcgtc tcgttgaact tcagcgtcat
    17281 caggtagaag acgagatctc ccatgcccag gtagccgaag acgatgccga ggaagagcag
    17341 cgtgaaggag atcgggaagc cgatgaagat cgacccgatc atggccgcga tcatgccgag
    17401 gccgatgatc tcgttgctca tgtctcggtg ctcccggact cggtcggcca gcggccggtg
    17461 cgcgcggcat agaggctctt aaggagctct gagatgcctt gaatcacgag caggagagct
    17521 gcaagcggga tgcaggattt gatcggcccg aggtagggca tccaggcggt ctccatgccg
    17581 cgctcgtatt cgacgaccga cttgaaggca taggcgtagg agacccagag gaagacgatc
    17641 atcgtcggga aatagagaac gatgtagagg agggcgtcga cgagcccttg gcggcggacg
    17701 ggccagctgc gatagaggaa atctgagcgg atatgcacgc cgcgcgagag gccgtacgcg
    17761 gcgccaagca tgaacagcgc gccgtaaagc atgcgtgaga tgtcgtaggc ccagatcgtc
    17821 ggcgtcaagg tcttgcggac gacgacctcg tagaccatgg cgatcatcag cggcacgacc
    17881 agccagctga cgaggcggcc gataaagcgg ttcgaggtgt cgatcgagcg gataggccaa
    17941 gacatccagg ccggcatgtc ggcgggcaag ccgccgggtc cgcgcgctac gggcgggctc
    18001 gcgctttgtc ccgcgtctgg gtctgccatg tgtcgttccc caatgcccgg cacgtggtgc
    18061 ccggcgtctc ggggcgccgc cccgcacccc gccaaaggcc ctggcctttg gaaaccaaat
    18121 cggcccgact atcgccctca agcgccaatg cccctcgagg gtcgctgtcg tggctgccga
    18181 ttgatgagtg gccccgcgat ccgaaccggc ggggccgcca cgcctacttc ttcttgaggg
    18241 ttgccgcgta ggcctcgagt aggttcgtgt tggcgcgctg cgcctgtgcc cagaacggca
    18301 ccgcagtgcc cgcaaaggtc ttcatggact cgaagacctt cttgaagaac gcattctcgc
    18361 cgctgtactt ggcgatcagc gtctggctcg ccttcatgta ctcggtgaag tagtcggccg
    18421 gcgtgtcgtg cagaatgacg ccgtgcttct cgacgagctc cttcagtgca agtccgttct
    18481 cgtggatgcg gtaggcgacg gtctgcatga gggaggcgtc agcggcgacc tcgatcgcct
    18541 tcttctggtg ctcggagagg ctgtcccaga ccttcttgtt taagtacatg tcggcattca
    18601 cgacgacctg gtgcaggccc tgcatgtagt agttcttgag caccttgtgg aagccaaagg
    18661 tcatatcggg tttcgggcag caccactcgg cggcgtcgat cacgcccttc tcgagcgcgg
    18721 ggagaatgtc gccgccaccg atcgcgacgg ccgcgacgcc cattaccttg tagctctcgg
    18781 ccgggatgcc gggcggagtc ctgaaccgca tcttgcggaa gtcgtccatg ttcttgatcg
    18841 gcgtcttgaa ccagccgagc gcctccgggc cgacgggctg gagcatgatg cctttgacgt
    18901 tgaccttcat ctccgtccag agctgctcgt agagcgcctt gccgccggca ttcatgtacc
    18961 aggagaccca ggcgaagttg tcgatgccga ggccggcgcc ggcgatcggc gagccgaaca
    19021 gcatggcagc ggggtgcttg ccggaccaga agtgcgtcca ggcgaggccc gcttcgacga
    19081 ggcccttgtc gacggcatcg agcgtctcgg cgacgccgac gattgcgcct gctggcagca
    19141 cctcgaacgt aacggtgcct ctggtcagcg ccgacacgtt ggcggcgaaa acctcgaaca
    19201 tgcgcacttc atcggccgtc tttgggatga cggcctggac gcggatcttg acgctcttct
    19261 cctgagccgc cgccggcgcg acaggcaccg ccccggcaag gccgagcgcg acggcaccgg
    19321 ccatgcccca cttcagacgt ttcatgaggt ctcctcccgg gagattggtt tcacgtcgga
    19381 acgattgccc accggagcag gccaacggct cttccggccg cttcgtatat cagggtggca
    19441 gtttcgggag ggttgggcaa gcgtcgactg agagcgtcga ctgcgaggct ggcctgagac
    19501 acgacctgtg gccatcgcgc tgcgctgatg gttagcgcgg tgaggacggc tgagccgagg
    19561 cagttcatcg cgcatgaatt gcatggccta catttcttgt ggctgaacac gggacgtgat
    19621 tgactgacgt cgccgctcgc caaaaaccga tactctcgac gccttgcgta tatcgtcagg
    19681 ggagcctgtc caatgatcat cgatacgaat atcagctgcg ttgggatgtc cgctcagctc
    19741 atcaaatgga acatcgacac tgtcggccgc tattatcgca aggcgacgca cccggagtgg
    19801 gtgatcacgc gggatgaggc gaaggaactc tcgaaagcag gcctcaagat tttcgccatc
    19861 ttcgaagact atggcctcgc aagcaagctc gttctgacga aagcgcaggg aaaggtcgat
    19921 gggaccagcg ccttggagca ggcggtccgg atcaagcagc ccattggcag cacgatctat
    19981 tttgcggtcg agggcttgcc gagcggctac aagtcgggcg acctcccggc gatccggaac
    20041 tactttgccg gagttcgcga cgcaatcggc ggcaagtatg ccattggcgt gtatggcgat
    20101 ggcatcgttt gcaagacact ggccaatgaa ggcatctgca agcacacgtg gctggctgcg
    20161 gcctcctgga gcttcgaggg caccaaggat ttcctcggcg actggacttg gtcgatcgcc
    20221 caattggggc ctctcgacat cggtctcggc aaactgagcg tcgatctcga tgtcgcaaac
    20281 ggcgatttcg gagcctttgt cgtgccccag gcttagtccg tccctcccat actctcgcga
    20341 accggcactg cgctcgcgcg agcagagccg gcaggtgcgc gccagaaagc gtagcgcggg
    20401 cttacggttg aggcgcgcaa ggcccggagc cgccgcgccg acgcgaccag tagggcgggg
    20461 tgaaaatgac gaccgtgtga caatgacggg ctaggccagc gcgccctcca ggaaccgcac
    20521 cggcgctcca ccggagggcg aggtgatggc gccctcccac atgacgcggc ggccgcgaat
    20581 gatcgtgccg accggccagc cggtgacggt gacgccatca tagggcgtcc aggcggcgcg
    20641 tgacttgatc cagccgttgg tgatggtctc gcggcgcttc aggtcgacga tcgagaggtc
    20701 agcatcatag ccgacggcga tgcggccctt gccctcaatt ccgaagatgc gctgcggccc
    20761 gtgcgaggtg aggtcgacga ggcgcatcag ggagaggcgc cccgcgctga cgtgatcgag
    20821 catgatcggc agcagcgtct gcacgccggt catgccggaa tggctgtcgg gataggcgtg
    20881 atgcttctcc tcgatcgtgt gcggggcgtg atcggagccg agcacgtcga cgacgccgtt
    20941 ggcgatgccg gcccagatgg cgcgccggtg cgcctcgtcg cggacgggcg ggttcatctg
    21001 cgcgaacgtg ccgagccgct ggtagcactc gggggcgacg agggtcagat gatgcggcgt
    21061 cacctcgacg ctggcatagg ccttgtggtc ggcgaggtag gccatctcct ccgccgtcga
    21121 gacgtgcagg acgtggacgc ggtggcggta tttctcggcg tagccgacaa ggcgctcggt
    21181 cgccatgagg gcggccacgg gatcgcgcca ctcggggtgc gacgtcggat cgcccttgcg
    21241 cctgagccct gcgcgctcgc ggagccgcat ctcgtcctcg gcatggaagg cggcgcgccg
    21301 cgagatgcgg gcgacgatct tctcgagcga gggtgcgtcc tcgaccagga gatcgccagt
    21361 ggacgcgccc atgaacacct tgatgccggc ggacgcctca agccgctcca gatgtgggat
    21421 atccgcgatg ttctcgcgcg tgccgccgac atagaaggcg aaatcgcagt gcatgcgatt
    21481 gcgggcgcgc gcgaccttgt cggcgagagt ggccgccgtt gtcgtgagcg gctttgtatt
    21541 tggcatctcg aacacggccg tcacgccgcc cagcacggcg gcgcgcgcgc cggtcgcgag
    21601 gtcctccttc tgctcgaggc ccggctcgcg gaagtgcacc tggctgtcaa tcacgccggg
    21661 gaggatgtgg agacctttgg cgacgactgt ctcggcagcc ttcaaggcgc cgagcgcgcc
    21721 gatggcaatg atgcgcccgt tcttgacggc gatgtcgcga atgccctcgc cgtcgtgatt
    21781 gacgaccgtg gcgccgctga tgatgaggtc tgcgcttggc gtgccggaca taaagccatc
    21841 cgagggttgt gcgttgtctc agtccttggc ccggtctata gcactccagc ttctcacgga
    21901 aaggagcttg gccatggccg catggatccg ccttgccgat cgcggtgtcg tttccgtcac
    21961 ggggcccgaa gcggcaggct ttctcgacaa tctgctgacg aacgatatgg cggtgctgga
    22021 gcggcagccc gcgctgctcg ccgggctgct gacgccgcag gggaaaatcc tgttcgagat
    22081 gcttgtcgtg aaggcgaatg acggcttctg gctcgagact gggcgggacg ccgcgccggc
    22141 actcgccaag cgcctcagca tgtacaagct gcgggctaag gtcgggatcg aggatcgctc
    22201 gggggacgca tgtgtcgctg tggcactcga cggcggcact gagcaaagag caggcagcgt
    22261 gaggttccca gacccccgtg cgccagggct cggcaatcgc atcattgccg tcgcgggcct
    22321 cgccgcagac ttgagcgatg agggccccta ccaccgccgc cgcatcgccc tcggtatcgg
    22381 cgaggcaagc cgcgactacc cgctcggcga cacctttccg catgaggcga actgggaccg
    22441 gatcggcggc gtgtcgttca cgaagggctg cttcgtcggc caggaggtgg tcgcgcgcat
    22501 gcagcacaaa acgatcgtaa ggaagcgctt ggtgcgcgtc agcggcgcag gcccgctcga
    22561 ggccggagcg ccggtcctcc ttggcgaggt cgagataggc cgcctcggta cggtagaggg
    22621 acgtgacggg ctcgcgctcg tgaggctcga tcgcgcgctc gaagcggagc cgtcgctcac
    22681 ctcgggcggc actgcgatca ccatcgatgc cgacacgctc gatcgctaca gacgcgagac
    22741 ggcggagcgg gacgcaagga gcaaggcgcc gctatgacgg aggctgtgac ccgctgcgcc
    22801 tggggcggca tcgatgatcc cgagtacacg cgttaccacg acgaggaatg gggggtaccg
    22861 aacagcgagg accgggcgct cttcgagaag ctcatcctcg aaggcttcca ggcggggctt
    22921 tcctggatca cgatcctgag gaagcgtgag accttcaggg ccgcgtttgc ccgcttcgat
    22981 cccgaggcga tcgcgcgctt tacggagacg gatgtcgctc ggctgatggc cgatccaggc
    23041 atcgttcgca accgggcgaa gatcgaggcg acgatctcga acgcgcgcgc ctatctggac
    23101 ctcaacgcgc gcactcggct ctcgaccttc ctgtgggact tcgtcgatgg ccggccgatc
    23161 atcaacgagc ggcgctcgat gaaggatata cccgccgaaa cggttgaatc gaagcgcatc
    23221 tcgaaagcct tgaagcagca cggcttccgc ttcgtcggac cgacgacggt ctacgcgttc
    23281 atgcagtcca tgggcttcgt caacgatcat ctgctgagtt gccatcgccg cgagccgtgc
    23341 gcgcgtctgc agcgggcgtt ccgcccgacg cacggctgag cgccagtcgg gtcaaaaccg
    23401 ctgcgctttt gcaggcgtac tgcttacacg tgtactgctg ccatcgtcga tcgtcttgcg
    23461 attgactagg ctccgggcgg ggatgatagt ccggggccag aaacggtgac gcactattag
    23521 gatcgcccga tgccttggct cgatattctc tccagcagat ccatgaccgc cgtctttact
    23581 cgcgggctca gtctcttggg cctgattgtc tttctgattg ctgccagtgc gccggaggcg
    23641 cgcgccgtcg attttccgat caaggcgaaa caggcgattc tgatcgacgc cgattcgggc
    23701 gcggtcctgt tccagaagaa tgccgacgac ctgatgtatc cggcgagcat gagcaagatc
    23761 atggcgcttg ccgtgatctt caaggcgctg aaagcgggta cgctgaagct cgacgagccc
    23821 atcctgatgt ccgagaacgc ctggcggaag ggcgggggcg tctcctacac gtcggccatg
    23881 atggttcccg tcaatacgcg cgagaaactc gagcagctca tcctcggcat catcgtccag
    23941 tcggggaatg acgcctcgat cgccatcgcc gaggccatcg ccgggagcga agaggccttc
    24001 gcgcgcatga tggaggagga ggcgcgccgg atcggcctca cgaagtcgac cttccgcaac
    24061 tcgatcggac tgttccatcc cgagcacaag atgaccgcgc gtgaactcgc gttgcttgcg
    24121 cgccatctgg tcattcagtt tccggaatat tatccgtggt tctcacagcg ggagttcctc
    24181 tatcgcaagc accgcttctt caaccgcaat cccctgctcg ggctgccgat cggtgtcgat
    24241 ggcatcaaga ccgggcacat caaggaagca ggcttcggca tcgtcgtttc ggccaagcag
    24301 gacaaccggc gcataatcct cgtcatcaac ggccacgagc gcgccgacga gcgcaaggcg
    24361 gaggcggtca agatcctcga gtggggcttt cggagtttcg gcgaattcaa gctgttcgag
    24421 gcgggcgagg tggtcggcca ggcgagggtt tggggcggta cgcgcatgta tgtgccgctc
    24481 tcgggtctga atggcggcgc cgtcgccgta gtactgccgc gcttcccggc aggccagaag
    24541 ctcaaggcag agctcatcta caagggcccg ctgaagccgc cgctcaagaa gggcgatgca
    24601 gtcgcgacgc tgcgtgtgac gtcgtcgtcg ggcgccacgg ccgagacgcc gctttatgcc
    24661 gcagaggatg tcgagccggc cggcatggtg cgccgtggct tcgacagcat gatgatgatg
    24721 gccttccgct ggatgatgtg agatcgcttt cgccggatcg tgagctgaga taagaccgcg
    24781 gcatgatacg cggccggttc atcacgctcg aaggcggcga gggtgcgggc aagtcgacgc
    24841 aggcgcggcg gctggcggat cacctggcgc ggcacggcat tgcgtcggtc gtcacgcgag
    24901 agcccggcgg ctctccgttc gccgaagagg tgcgccgcct cctgctctcc ggcagcctgc
    24961 cctcgcacgg ggcgctttcc gaggcgctcc tcttcgcggc cgcgcgcgcc gatcatctga
    25021 gccggaccat ccgccccgca ctcgaagccg ggcgctgggt gatctctgac cgcttctccg
    25081 attcaacgcg cgtctatcag ggtgccgccg gaggcctcag cccggacgtt cttgcgcggc
    25141 tcgaggcgat cgtgcccggc tcgacgctcc ccgatctgac cctcgtcatc gatctccccg
    25201 tcgatatcgg cctcgcgcgt gccggcgccc gcaatgccac ggcggagcgc gatcccttcg
    25261 aaggacgcga acgagccgcc cggcaccgtc gtcgaagtag cgccggcgcg cgttgccgtc
    25321 atcgacggcg accgtgaggt ggcagccgtc ggcgcgcaga tcgcggccct cgtcgatcgc
    25381 cgtttcggtt tgaaggcacc ctgatggcgc gcgcgcagac gattgcggac gtcgaggcgc
    25441 tgccggaggc tgatcggctc gacggtgcgc cgcatccgcg cgagaccggt gagctcgtcg
    25501 gccatgtcgg cgccgaggcc gagctcgagg cggcattccg gtcgaggcgc atgcatcacg
    25561 cctggatcct tggcggcccg gccggcatcg gcaaggcgac actcgcctac cgctttgcgc
    25621 gttacctgct cgcggcgccc gaggagcggc ctgaggcggg gctcgccatt ccgcaggagg
    25681 cacgcacgag ccggctggtg cgccagctcg cgcacccgcg cctcgccgtc atccgccgga
    25741 cctacgatgc gaagacgaag cgctttccgg cctcgatccc cgtcgacgag gtgcgtcggc
    25801 tgcgcgcgtt cctcggtcac accgccggcg agggcgagtg gcgcgtcgtg atcatcgaca
    25861 cggcggacga actcaacgtg aatgccgcca atgcggtgct caagtcgctc gaggagcccc
    25921 cgccgaggac gctattcctg ctcgtctcct cggagcccgg acgcttgctc gtcaccatcc
    25981 gctcgcgctg ccgcacgctg atgctcgcgc cgctctcggg cgccgacctg cgcaaggcct
    26041 cggaaggcgt atttcaggac ggccggccga tcccgcccga ggactggcca cggctcgaga
    26101 tcctggcgaa cggcagtgtg cgccgcctcc tcggcctcct cgaaagcgag ggacttgcgc
    26161 agcatgcggc gatcatgaag ctgctgacgt cgctgccggt cgtcgactgg acggcggtcg
    26221 agcagctcgc cgaccagctg acctcgccgc aggccgagca gcgcttcacg tcgttctacg
    26281 atctcctgct cgacgagatc gccaggctcg tgcgcgcggc aggcggggtc agcggcagcg
    26341 cggagggcct cgcgctggcc gcgaagctcg gcgttccggc tcggcttgcc acctgggccg
    26401 gtgtgtggga aacagtcgtc cgctcgaagg ccgagacgct tgcgttgaac ctcgatcgca
    26461 gaacattgat catcgagacc atatcccggc tgcagtcggc agcccggccg tgaggcagcg
    26521 aagcgcaatc cgtcggcgct cggcgcgtcg gccgaggcga gcctcccctg ccgcgaggac
    26581 tgcagcagga ggccggtgat ggccacgtcg aatcgctact acatcacgac gccgatctac
    26641 tacccgaacg gcgtcccgca tatcggccac ggctacacga cgattgcctg cgacgtgctc
    26701 gcgcgcttca accggctcga cgggcgcgac gtgtacttcc tgaccggcac cgacgagcac
    26761 ggcctcaaga tcaagcagac ggctgagggc cggggcatca cgccgcagtc cctcgtcgac
    26821 gagctctcgg cgcgcttccg cgagatgtgc aagcagctcc acatttccaa cgatgatttc
    26881 atccgcacga cggaagcacg ccacatcgcc tcgtgccagg aactctggaa gcgctgtgcc
    26941 gcggccggcg acatctatct tgaccgctac gccggctggt attccgtgcg ccaggaggcc
    27001 tacttcgacg agagcgagac gagtgtcggt cccgacgnnn nnnnnnnnnn nnnnnnnnnn
    27061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagcgg
    27181 cgcaacgagg tcgtctcgtt cgtcacccgc ggcctcgagg atctctccat ctcgcgcgcc
    27241 aagctcgact ggggcatccc ggtgccgggc gatccgagcc acgtcatgta cgtctggttc
    27301 gacgcgctga cgaactacat ctcagcggcc gggttcccgg acgaaaagca catgctcttc
    27361 cagaagtttt ggccggccga tctgcacatg gtcgggaagg acatcgtgcg cttccacacc
    27421 gtttactggc cggccttcct tatgtcggcc ggagtggcgc cgccgcgacg tgtcttcgcg
    27481 cacgggctgc ttctcggcaa ggccggcacg aagatttcca agtcggaagg cgcgccgccc
    27541 atctccgaga tcatcgagga atacggcgtc gacgccatcc gctatttcgt tatgcgcgag
    27601 gcctctttcg gccaggacgg tacctatacg aaggagacgc tggtcaatcg catcaacgcg
    27661 gacctcgcca acaacttggg caacctcgcc cagcgctcgc tgtcgatgat caacaagaac
    27721 tgcggagcgg ttgtgccgac gccgggcgcg ttcacggcaa cagacacggc gatcctcgcc
    27781 gcagccgacg gcatgctggc gcgcgcccgc gaggcgatcg agcggcaggc gatccacctc
    27841 tatctcgagg ccgtgtggag cgtcgtcgcc gatgccaatc ggtacttcgc cgatgagcag
    27901 ccctgggcca agaaggcgac cgaccccgcg cgcatgggga cgatcctcta cgtgacggcg
    27961 gaggtcctcc ggcaggtggc gctcctggcg caggcgatca caccggcagc ggcggggacg
    28021 ctgctcgatc tcctcgcggt gccggccgat cggcggaact tcgcgagcct cggcacggcc
    28081 gggcgtctca gcgcgggcgc ggcacttccc gagcccgcgc ccgtgttccc gcgctatgtg
    28141 acgcccgtgg agcgcgaggt cgccgccgcc aaagcggcgc agggcaaggg cgcgcccaag
    28201 ggcaagacgg cgaaggccaa gcctcagggt tgagggtgaa agggacacct gcaccgccca
    28261 caattcttgt gagtgaatga aagtgccgat ggcgctggat ggctgggttt tcgcctctgg
    28321 cgctcagctc gtgcgtacgg atcgtacgat cgagctcagc aacccacatc ctgccggcac
    28381 ctagagtgac ggtgccagac gcaggtttct ggcgccgctt ttacgtaccc acgggcagtc
    28441 gcccgctggc ggctgagggg tgatccgcgg gtgattgcca gtgccgatcg gccctgttag
    28501 aaggccttcg atcatgagcc agaggtggcc ggccgatgcc cttcgattca cgcgatccga
    28561 acggggctcc cccgtcaatc aagctcgtcg ccggcaacag caacaagccg ctcgccgacg
    28621 cgatctcaca gcacacgaag attccgctca ccaaagccgt ggtgaagcgc ttcgccgata
    28681 tggagatctt cgtcgagatc catgagaacg tgcgcggcca ggacgtctac gtcttgcaat
    28741 cgacgtcgtt tccagccaac gacaatctga tggagatgct gatcgtcgtc gatgcgctga
    28801 agcgctcctc ggctcgtcgc atcaccgctg tcatccccta ctacggctac gcccgccagg
    28861 accgccgccc gggcccgcga accccgatca cggcaaagct cgtcgccaat ctcatcgagc
    28921 gggccggcgt cgaccgcgtc atgacagtcg acctgcacgc gggccagatc cagggtttct
    28981 tcgacatccc gaccgacaac ttgttcgccg cacccgtcat catgcgcgac atccaggagc
    29041 gcttcgacgt gtcgaacgtc gtcgtcgtct cgccggatac gggcggcacg gtgcgcgcac
    29101 gcgggctcgc caagcgcatc ggcgccccga tcgcgatctg cgacaagcgc cgcgacaagc
    29161 cgggctcggc cgaaatcatg aacgtcatcg gcgacgtcgc cggcaagcgc tgcatcctgg
    29221 tcgacgacat cgtcgattcg ggcggcacac tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29341 nnnnnnnnnn nnnnnnnnnn ngattcgatc ctgccgaccg aggctgttca cgcggcccgc
    29401 aatatccgcc tcctctcgat cgcgccgctg ctcggcgagg caatcctgcg cacctcacgc
    29461 gaggagagtg tgtcgagcct cttctactga ttgcgtctcc cgatatcgac cccgacacac
    29521 gacacctcgg ccagcagcga attgcggcgg accctttcgg atccgccgct cggcaaacag
    29581 cgaaacaggc gtctgcaata ggtgtaggta tcgcaatggg gcatcgaccc gggggaaagt
    29641 cgatgccagg gaccgaggcg atgggggagg ggcatctctc ggcttacccg aaccatgcga
    29701 cggccagagc gatttttctg cgcgacccca tccacattcc gattgtcgcg tcggctggcg
    29761 gcagaacggc gaacgttgta gctcctcgac aggcgacgcg gcggaggggg catgccgagc
    29821 tctggtgatg ggatgtcgaa ggcgctggcc gtcctcgaga cgacgttcgg ctacaaggcg
    29881 ttccgctcgc atcaggccga gatcgtcgag acgattatca gcggcgggga cgccctggtc
    29941 ctgatgccga ccgggggcgg caagtcgctc tgctatcagg tgccggcact cgtgcgcccc
    30001 ggaacggcca tcgtcgtctc gcccctcatc gccctcatgc aggaccaggt cgacacgctc
    30061 cgcgagctcg gcgtcgaggc acagttcctc aactcgacac tcggctggcg cgagcagcag
    30121 gacgtggaga gccgcctcct ctcggggaag ttgcaactcc tctacatcgc ccccgagcgg
    30181 ctgctgcagg aacgtacgct tgccctcctc gacaaggcga agatcgccct catcgccatc
    30241 gatgaggcgc attgcgtctc gcagtgggga cacgatttcc gccccgagta ccgccagctc
    30301 aaggcgctgg ccgagcgctt tcccggcgtg ccgcgtgtcg ccctcacggc gacggcggac
    30361 gcgcggacgc gcgatgagat ggtgcacgag ctccgcctcg aaggcgcccg ccagttcgtc
    30421 gcgagcttcg atcgccccaa catccgctat acgatcgccg agatgggctc gctcagcggc
    30481 cgcgagcaac tctggcgctt catctcccgc gagcatcccg aggacgcggg catcgtttat
    30541 tgcctttcgc gcaagagcgt cgaggagacg gcggcgttcc tcgccgcgcg tggccgcaag
    30601 gcgctcgcct accacgccgg cctcatgccg gacgcccgcc gcatcgcgca ggagcgcttc
    30661 ctcaaggagg acggcctcat cattgtcgcg acgatcgcct ttggcatggg catcgacaag
    30721 ccggatgtgc gcttcgtcgg ccacttgaac ctgccaaagt cggtcgaggc ctactaccag
    30781 gaaacgggtc gcgccggccg cgacggcgag gcagcgaatg cctggatggc ctacggcatc
    30841 caagacatcg tcacgcagcg ccagtggatc cagcagtcgg aagccggcga cgagcacaag
    30901 aaggtgcagc gccagaagct cgatgcgctc gtggcgctct gcgaggtcgc gacctgccgc
    30961 cgccaggcgc tgctcgccta tttcggcgag acgctacctg agccctgcgg caactgcgac
    31021 aactgcctga cgccgcccga gaccgtcgat gcgacggtgc tcgcgcaaaa ggccctctcg
    31081 gccgtctacc gcaccggcca gcgctacggc atgagttacg tgatggacgt gctgctcggc
    31141 aaggcggacg accgcatcgg ccgcaacggc catgaccgcc tctcggtctt cggcatcggc
    31201 cacgaggagg gcgagaggga atggcgcgcc gtgttccgcg cgctggttgc gggc
//
LOCUS       DAORNH010000015        30148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1168_length_30148_cov_2.644569, whole genome shotgun sequence.
ACCESSION   DAORNH010000015 DAORNH010000000
VERSION     DAORNH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 30148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30148
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1168_length_30148_cov_2.644569"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1252)
                     /locus_tag="PK264_02955"
     CDS             complement(173..1252)
                     /locus_tag="PK264_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD74886.1"
                     /translation="MKKMMWLAAVGATAVTAVATAPSASAQVETVRVGWCAKTVSSAA
                     APFAIALKMGWFEKEGIKVELVPLPGSGECVKFVATKEIPFSLPSVEPVAVLRAQGAK
                     LKFFYTAYQGNVYGMAVPADSAYKTVADMKGKKVGVISMASASAIIARALAKEAGLDP
                     EKDITIVVAGEAGQTAALLKNGSVDVLSQFDTQYALVENAGFKLRRLSHPSIDKFPSN
                     AFLALEETIAAKRKQAVALAKGYAMGTHFAVTNPEAAIRIMWEIWPQTRATGKDEATA
                     LRHDIATLMARAPAWSLEKVGAKKWGEHLVANYQAYLEWLLANGIIKVKANAADFLTD
                     ELLADVNTFDANAVAAAAKAWTVKK"
     gene            complement(1249..2052)
                     /locus_tag="PK264_02960"
     CDS             complement(1249..2052)
                     /locus_tag="PK264_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD74887.1"
                     /translation="MGGVKHFSLRLLLIAAVLGAWELLVKVFAIEAYILPPPSQVFRA
                     LYEGIARNIYPEHLGVTLLETMLGFLLACVLAFSLGILVALSRRVEYFLYPFIVMFQA
                     MPKVALAPLIIVWFGLGLKSKVINAALVAFFPLMVNTIVGLRSAEEDRINLMRSLAAN
                     KWQIFWMLQLPNALPYIFAGLEIAMIFALIGAIVAEFIGAERGLGMLLQSMNMNVDVA
                     GQFSILLILSMVGLILNGTVGAIRRRLLFWDPAQAAEQGQSKSSKGGSV"
     gene            complement(2052..2897)
                     /locus_tag="PK264_02965"
     CDS             complement(2052..2897)
                     /locus_tag="PK264_02965"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD74888.1"
                     /translation="MDGTGVSGQGVQSGADLGTIATAASGPAVISVDQLSLAYGRNAD
                     AVLALSDISFSIADGEFISIVGPSGCGKSTLLKILAGLLPPTSGEAMLKGTPIRGARR
                     DIGVVFQSPVLFPWRSVLGNVLLPADVQGLDKKAMRARALDLVRLVGLEGFENRYPRE
                     LSGGMQQRVGIIRALIHDPAILLMDEPFGALDAMTRETMNVELQRIWMESRKTVLFIT
                     HSTSEAVYLADRVIVMTSRPGKIGDAFKVELPRPRKLSVMNTELFGGYVRRIRTALNA
                     DGGLD"
     gene            complement(3090..3527)
                     /locus_tag="PK264_02970"
     CDS             complement(3090..3527)
                     /locus_tag="PK264_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74889.1"
                     /translation="MGSTSVLHVSSGLFLTGVGGWRKDENHRELIGAWDNREPDSTFW
                     HLDGGISKNDLGIGRTVFDGEYAPHTDGMRHRFSDVVSSDVMFWGLGMVQHIDAAASE
                     LFIADKHHDATFTRNAGTLAAPVFESVAANDFSAVVLGTRIKF"
     gene            complement(3527..4270)
                     /locus_tag="PK264_02975"
     CDS             complement(3527..4270)
                     /locus_tag="PK264_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HRD74890.1"
                     /translation="MARLARRIGEKRFLKIVRAVLNAGMMQGGVVIGRHEGTPEGGPL
                     SPVLAELLLDDLDQDLEQRGHRFCRYADDCIIYVRSKAAGKRVMAYVTAFIEGHLRLE
                     VKDKKSAVAPDEERSFLSHRYLSSGEDRSRTERPHPREAAASTVGPAQPGHRVGADVR
                     RIERLHGGMGHVAPPRRLEGRSGRAPRMASMQTSVSAQLARVLPISAASGSPRASAIG
                     RIASSTGRTSFSMRLACCAHRSANGCRGS"
     gene            complement(4404..5591)
                     /locus_tag="PK264_02980"
     CDS             complement(4404..5591)
                     /locus_tag="PK264_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD74891.1"
                     /translation="MSQRALPLDGIKVIELCHVVMGPTCGMILGDLGADVIKFEPAPD
                     GDNTRRLIGSGIGFFATFNRNKRSIALDLKSPEGLEVARRLIASADVVTENFRPGALD
                     AMGLGYDVCKTVNPRLIYCSLKGFLTGPYQERAALDEVVQMMAGLAYMTGPRGRPLRA
                     GSSVNDIMGGMFAAIAILAALRERDRTGAGQKVVSALFESCALLSAQHMMQYAVSGKP
                     APPMPERLAAWGIYDVFDCADGQLFIGVVTDTQWKLFCEAFELADMYADPGLATNVKR
                     CGRRDEFMPRLREQFASWRLADLEAVVAKAGLPYAPIVRPEQLFDDPHLTASGGLLPI
                     TLPDGRETVTPALPIELDGRRLGVRRGLPGIGEHGREVLAEIGIGVAEIERLEAAGTL
                     GKR"
     gene            5747..>5991
                     /locus_tag="PK264_02985"
     CDS             5747..>5991
                     /locus_tag="PK264_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74892.1"
                     /translation="MLLICLGLTASFAYHIRHGRHGLEARSTLLARSAALELRVHALE
                     TVRARLARDIALLGAAPDRDLVSELAQELVDLLLGAD"
     assembly_gap    5992..6118
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6119..>6336)
                     /locus_tag="PK264_02990"
     CDS             complement(<6119..>6336)
                     /locus_tag="PK264_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74893.1"
                     /translation="ERLDHRPGQLSGGEQQRTAICRALANAPRIVLADEPTGNLDPTT
                     SEGVFRELIGLIERTGVAALIATHNLEL"
     assembly_gap    6337..6346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6347..6741)
                     /locus_tag="PK264_02995"
     CDS             complement(<6347..6741)
                     /locus_tag="PK264_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74894.1"
                     /translation="MAILELVDIRRTFRQAERDLHVLVGASAKLEPGQAVALVGPSGS
                     GKSSLLHVAGLLERPTAGQVIVGGRDCADLDDGARTAMRRSTIGFIYQFHQLLAEFSA
                     RENVMIPQMIAGKARGIAAERAGELLTLMG"
     gene            complement(6753..8000)
                     /locus_tag="PK264_03000"
     CDS             complement(6753..8000)
                     /locus_tag="PK264_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HRD74895.1"
                     /translation="MFAPVEWRMAIRYLLPRPTWRWMPPLRTFLSFLGVMLGVAALII
                     VMAVMNGFRQELLEKILGLSGHVVIQKIGAPFDDHADAVKRLTGVPGVKSVVPLVGGQ
                     VIIASQSQTLGGFVRGMSEANAKILPSVGKNIKFGSLDNFDSGEGIVIGTRLARDLGV
                     TIGDGVTLIAPRGAQTPFGTVPANKRYTVTALFEMGMSEYDRTVIFMPLGEAQRYFRK
                     GANIDAIEVTVDNPQAMDKVLPFIEMAAGPSLHLADWRRQNSEFFTVLEVERTMMFII
                     VSLVVVVAALNIVSALTMLVRSKSRDIAVLRTMGATKWSVMRIFLIAGATIGVVGTIA
                     GLLLGIVFCHYIDQIRLFAQWATGVVLMNPKIYYLTRMPALIDVGEATRSVIFAITMA
                     VLAALIPAWRASRLDPIEALRYE"
     gene            complement(8182..9528)
                     /locus_tag="PK264_03005"
     CDS             complement(8182..9528)
                     /locus_tag="PK264_03005"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRD74896.1"
                     /translation="MRLSRYFMPVLRETPKEAEIVSHQLMLRTGMIKQAAAGIYSWLP
                     TGFKVLKKIEQIVREEQNRAGAIEVLMPTVQSADLWRQSGRHNSYGPEMLRFTDRHDR
                     EMLYGPTNEEQITEIFRDGVRSYKDLPRNLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLTEEGARASYNRMFVAYLRTYARMGLRSIPMAADTGPIGGDLSHEFIILADTGE
                     SQVYCHKDLIEGAIPPAGTDFNGDLSPIIKAWTSKYAATDEKHDAAAFDRDVAKDKQV
                     TARGIEVGHIFYFGDKYSKPMGCRVQGSDGKLVNVQMGSYGVGVSRLAGAIIEAHHDK
                     DGIIWPVPVAPFEVALVNLKAGDADTERVCGSLYEKLTNAGIDVLYDDTDERAGVKFA
                     TMDLIGVPHQLIIGPKGVKAGEAEIKARKGGARRTLPIDAALSAVIADVTSQRVLA"
     gene            complement(9657..9863)
                     /locus_tag="PK264_03010"
     CDS             complement(9657..9863)
                     /locus_tag="PK264_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HRD74897.1"
                     /translation="MNRADDRELRELLLRLRAEHRDLDAEIVVLETSSTSDQLQISRL
                     KRRKLKLKDQITAIEDRLTPDIIA"
     gene            10076..10537
                     /gene="aroQ"
                     /locus_tag="PK264_03015"
     CDS             10076..10537
                     /gene="aroQ"
                     /locus_tag="PK264_03015"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRD74898.1"
                     /translation="MTKKPILILNGPNLNMLGTREPHIYGATTLMEVKAMCEARAKAR
                     GHAISFHQSNKEYELVEWIQGSISTAAGIIVNPAAFTHTSVAILDALSMFKGPIIELH
                     ISNPHKRESFRHHSYVTQAATALICGLGVKGYPLAVDAMADLLEAASAPKA"
     gene            10647..>10880
                     /locus_tag="PK264_03020"
     CDS             10647..>10880
                     /locus_tag="PK264_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRD74899.1"
                     /translation="MFDFDRVIERRGTHASKWDNMAKLSGITAPDAIPMWVADMDFPA
                     PPGVTAVLAGEVGRATHGYYADTGSWTESMVEWM"
     assembly_gap    10881..10892
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10893..11830
                     /locus_tag="PK264_03025"
     CDS             <10893..11830
                     /locus_tag="PK264_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD74900.1"
                     /translation="KVDPSWVSATPGIVSGLGLIIQALSEPGDDIVVFPPAYHAFRKI
                     ILANERRILDVGLVERNGRYAMDLDLLRSRLTPRTKAVFLCSPHNPGGTVWSPNELRD
                     LAKVCAERGVVLVSDEIHCDLVFPGARHTPTIAAAPELAENIITCVAATKTFNLAGAH
                     VGACFTSNRTLKKRLDARIAASGLASYNSFGMLATEAGWRSGEAWLDALIPYLKANRD
                     EFDRRIEKAAPGARSMRLDSTYLAWVDFAKTGLAPADVAERVAKRARIYASPGEQFGP
                     GGANSLRFNFATPRPILNEALARLEEAFRDIRTGA"
     gene            11844..12611
                     /locus_tag="PK264_03030"
     CDS             11844..12611
                     /locus_tag="PK264_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRD74901.1"
                     /translation="MRPVIGVIGNTHVIENRFPAQLAGERNLRAIAEVCEALPLVFAG
                     CPEITDIGSLLETVDGILLTGARANVHPTRFRTEPNPKHEPYDERRDAMALAVINACV
                     ARSIPVFGICRGFQEMNVAFGGSLHPEIREIPGRMNHRMPRLPTGEIHPDQTVVFADR
                     HDVRLKPGGHFATILGTDCIRVNSLHGQGILEPGQRVVIEGVAEDGTAEAICIEGAAG
                     FALGVQWHAEYDPQRNPVNRALFKAFGDAVKSYRHAS"
     gene            12746..>14095
                     /locus_tag="PK264_03035"
     CDS             12746..>14095
                     /locus_tag="PK264_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD74902.1"
                     /translation="MSLSVEAAPAHRRLGSWFLIAFPHLGALLILLATETSLVGRFAF
                     VLAWAFFTLGWMAVLRRPAVAGMLALGFLVLLVLLSRFKHEILLMTVNFVDVMLIDAD
                     TIAFLLQVFPRLDLKAAAGAIVVAAALAMVWRLDRARVRRSLAAGGAAASLGGLVCLS
                     VLVPNDPWEEFYAENYFSKYARSAVTAISDLITRGVLDSDKTATERLAASPSTPCRLD
                     KPPHIIMVHDESSFDARAIPGLKVPKGYGDHFVSGDGKRRTLLVEGPGGPSWYTEYSI
                     LTGLSARSFGRFVDFTTRIAVGRIERGLPRALARCGYKTFTLYPMYGTFLSARAFQTT
                     AGIQSFIDAQSLGAKFQDADSFYFDAAAATLAKHRAEGPLFVLVYTAQNHFPWTFRFR
                     PDLAPDWTAPGNRADVDEYLRRQHLTAVDYQAFLARLKREFPGEPFLIVRFGDHQPYF
                     "
     assembly_gap    14096..14206
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14207..14403
                     /locus_tag="PK264_03040"
     CDS             <14207..14403
                     /locus_tag="PK264_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRD74903.1"
                     /translation="LDTLDVPYLALVVLEAAGVPLDPSFTEQKLILERCRGIFHRCNG
                     GAEARRFNRLLIDAGLIKGL"
     gene            complement(14390..16558)
                     /locus_tag="PK264_03045"
     CDS             complement(14390..16558)
                     /locus_tag="PK264_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD74904.1"
                     /translation="MATWSALEARGQSPALKSDSAGPTALREPKTFSLHGTSWVDEYH
                     WLAARNWNEVRSDPTKLEKRIRDHLEAENRHTAATLAETRALQAKLYAEMRGRIVEAD
                     TSVPLTYGAWSYYRRTVKGGQRFKMVRRPLAGGAEEVLVDGDRLAAGSKEFSLFDKWL
                     PSPDHTRIVYAVDPVGSESYTIRVRDLRSRRDLPDSIPNTNGRAVWSLDGQWLYYVRL
                     DDDSRPLSVYRHRLGTSPSADILVYKETDLGFYVDVSRLASGRYIAIESGTHQTSEIR
                     LVDARAPEAPPRMVAARRDNIRYSVEIVGERLITLTNADGALDNKIIEAPLASFETSP
                     PRELVPHVPGRVITWLETLDDQLIWLEETDGLPRIMVRRLSDGREHAVSFGDGPHVLG
                     IHSGYEQSSTELIVSFEAPTTPVRYWKHDVTTEKRVVIKETRIPSGHRPDDYVTRRLM
                     AKAADGELVPITFFHRADRAGDPDAPLYLTGYGAYGVASKPRFERDILSLVDRGFTMA
                     IAHVRGGNDRGERWYVEGRGEKKPNTFSDFIAAAEHLIAEGHVKRGNIVIAGASAGGM
                     LVGAVANARPELFLGVIADVPFVDVLATMLDASQPLTQLEYPEWGNPEASKSAFDLIR
                     SYSPYDNVRAQPYPHILATGAISDRRVAYWEPAKWVARLRAMRTNRNLLLLHTNFGGH
                     GGPPGRFERLRDEAMRYAFALKITGRTRAELPRRARTKAP"
     gene            complement(16713..17228)
                     /locus_tag="PK264_03050"
     CDS             complement(16713..17228)
                     /locus_tag="PK264_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74905.1"
                     /translation="MSQRRLAISCLSSLLGLLGAAGGTAAQTRAWPVYQNDRYGFTIS
                     YPGEIFKAAGPGRPDGQLFVSVDGKAKLLVSASVNSTNETVESYRAFVLRTAYPEARL
                     SYGRRDNRLFIVSGFKGDQIFYERVEFGCRGRIISGWQILYPAAERATYDSVVERMSR
                     SWKPVSERRCQ"
     gene            complement(17347..>18075)
                     /locus_tag="PK264_03055"
     CDS             complement(17347..>18075)
                     /locus_tag="PK264_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74906.1"
                     /translation="YIRRRTIEPVAERMERLWEGITRRVFGLLARLALVISFATTSSI
                     FLLGGTFAAIGIDLFEAIDHASIFQTVALHAVAIASIVVLVRALGFLAQDQMPELEVW
                     KHATNVMPMALAFAAVAVWYLPLAIAGPLIAVTCVGCWLTARISITAMVIFGTLAFFP
                     VGIARAVSLHQGEAPTLLTRAGRAEPFTIIVSANRGLLGFAGTDKTVEFVPWEQVQSL
                     RSTRSRGWLGWSPAAGPIGTGRGK"
     assembly_gap    18076..18112
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18361..19407)
                     /locus_tag="PK264_03060"
     CDS             complement(18361..19407)
                     /locus_tag="PK264_03060"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRD74907.1"
                     /translation="MRSNGPDDAPARITRRAALGLGLSAAGAAGLPVPSSEAARYTAG
                     RKMAPGEFAWTPERSPSGPVVMIVSLPEQLVHIYRNGIEIGVSTCSTGRPGHKTPTGV
                     FTILEKQRKHVSSIYKGAQMPNMERLTWGGIALHAGNLPGYPASHGCVRLPLKFSALL
                     FDVTHLGVVVIIADERTQPASVVHPAALLPDAAAVEATSVAEHVAKRRAHGAWDATVT
                     YPTTHVVISRRDGKAIIVTDGKVEGSYPVTFKSPARPLGTHTYSLVGPTADGKALSWL
                     AFGIGKSSREAHIVTNLSDTAMRRVVFSDPERALRIARMFHPGTTLLLTDASAPGSSR
                     HTGDDFAIIASEPV"
     gene            complement(19517..>21059)
                     /locus_tag="PK264_03065"
     CDS             complement(19517..>21059)
                     /locus_tag="PK264_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD74908.1"
                     /translation="EALVRDLDQHRRDATGGGPDKLKQRHVARGKLLPRDRVLGLIDP
                     GSPFLELSQLAAHGMYTGDIHGAGVITGVGRVAGRECVIVCNDATIKGGTYYPMTVKK
                     HLRAQEIAIENRLPCLYLVDSGGANLPHWTEVFPDREHFGRIFYNQARMSASGLPQIA
                     VVMGSCTAGGAYVPAMSDETIIVRRQGTIFLGGPPLVKAATGETVSAEDLGGAEVHTR
                     SSGVADHYALNDDHALALARTIVKSLPPPRRPDVNLIEPRPPAYDPAELDGIVPAALT
                     TQYDTREIIARLVDGSELDEWKRNFATTLVTGFAWIEGMPVGILANNGILYSETAQKA
                     AHFIEICCQRRIPLLFLQNITGFMVGREAETGGIARHGAKMVMAVACANVPKLTVVIG
                     GSYGAGNYAMCGRAYGPRFMFTWPNARVSVMGGEQAASVLATVRRDNIEAEGKSWSKS
                     EEQAFKKPVLEAYERDGHPYHGTARMWDDGVIAPSETRRVLALSLAASLNAPIEPTKF
                     GVFRM"
     assembly_gap    21060..21328
                     /estimated_length=269
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21329..21602)
                     /locus_tag="PK264_03070"
     CDS             complement(<21329..21602)
                     /locus_tag="PK264_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74909.1"
                     /translation="MSETASPEAAPAMSPADRERQERLIRRMRLVVIVLGILLVIGFF
                     SLVGRILYLAFRGPPQAVSPRPALAMPTAPAPPELTLPLPAGAAVTS"
     gene            21686..22693
                     /locus_tag="PK264_03075"
     CDS             21686..22693
                     /locus_tag="PK264_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRD74910.1"
                     /translation="MSQGAVSHTVMIADEQAGLRLDRALAAALPDLSRQRLQALMRAG
                     AVRRAGRTIDDPSARVKPGDVYTIDVPEPEAATPEAESIALDIRYEDDHLIVIDKPAG
                     LVVHPGAGHASGTLVNALIAHCGTSLSGIGGVKRPGIVHRLDKDTSGLMVVAKSDASH
                     RGLQEQFASHGADGLLTRAYLALVWGQPLRPSGVIDASLQRSRADRTRIAVTTSEEAR
                     HAVTHFKVIERYVDAKGKPLASLLRLELETGRTHQIRVHLAHISHPVMGDPVYGAGYK
                     ASAGRLTQAARAALEALGRQALHATHLGFEHPISGKSISLDSALPVDIARLRDLLRMP
                     D"
     gene            22946..23365
                     /locus_tag="PK264_03080"
     CDS             22946..23365
                     /locus_tag="PK264_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD74911.1"
                     /translation="MAGVAPRPRPPTIELSDPDAASLLPPQQIKAQIEHYVAVAFAVT
                     LDDLRRPSRGPAEAAFARQIAMYLAHIACGWTFTEVGRLFERDRTTVAHACALIEDSR
                     DDISLDRALEWLERLVRRLASPRRLAREWRSALDDSD"
     gene            23460..24764
                     /locus_tag="PK264_03085"
     CDS             23460..24764
                     /locus_tag="PK264_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD74912.1"
                     /translation="MPLSAVSGTLTTLTLLFVIASSVSSGFTQQKSRTDTKSANSSSI
                     ESAETLLTSGNDEAKRGNYEAAIKAFKSAHDAFKKASEKSSKKEEPLYNMTYANERIG
                     DVHLVAGKHEAAIAIYSAVLVERGKLAKTFPKSSLMMAGVGFAHERLGTAEFDRGNDS
                     EALSRFNNFHDLLKALSESDSKDIRWHRGHSISHTKLGAVYRKQRINKQAIDNLKAAA
                     NIAKAVAADWPKEPGVDEDLYSALLELGEAYRVSGEYLSAKSSYEAALPVIRRVADAA
                     PKDAYALNSLGVVNDRLGDMMMFSGNPKAAIEFYRTAYELIQMAQGGSTKSTVIEFNS
                     VIMLFTLALAEGLSGSDKNAEIAKLYTEFRNLLDLKTEQSAQKRSYLASELFRLVVNP
                     VVARQRLVESMAVYDRQDKSRKLRDDERLWYRVIDSGLRALP"
     gene            complement(25073..26275)
                     /gene="rnd"
                     /locus_tag="PK264_03090"
     CDS             complement(25073..26275)
                     /gene="rnd"
                     /locus_tag="PK264_03090"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRD74913.1"
                     /translation="MRDIRAAFDYTIVMRLIETTTELAAICDRLANERFVAVDTEFMR
                     EQTFWPQLCLVQLAGPSPNQEFLIDPLAGGIDLAPLVSLMTDRPPVKVFHAARQDIEI
                     VHIMSGRVPAPLFDTQVAAMVCGFGESISYVNLVKRVLGQDLDKSSRFTDWSRRPLSQ
                     KQLVYALGDVTHLRDVYVQLDEKLKSTGRHAWLAEEMAALGDPETYVLRPEDAWKRLK
                     LKVRNRRSLAVLIELAAWRDGLAQSLNVPRNRVLRDEALYDVANQAPTDVAALSGLRT
                     LSDGFARSQRAKEIIEVVKRGLARDLKTVPPIPGGPALSADANALVDLLRVLLKAAAA
                     RHSVAPRLIADADELERLATEDEPDIAVLKGWRRELFGADALRLKRGEVAIVVRKGEV
                     VTVDAPKA"
     gene            complement(26321..27448)
                     /locus_tag="PK264_03095"
     CDS             complement(26321..27448)
                     /locus_tag="PK264_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRD74914.1"
                     /translation="MKIQGAVLYEQGLSRPYATSKPLRIETLDLEGPRAGEVLVKVKA
                     AGLCHSDLSTIEGARTRPLPTLIGHEGAGVVAALGAGVSDLAVGDHVVFIFAPSCGAC
                     RQCVSGRPNLCTTFNAAKQKGDLATGGRRLSLNGTPVAHYAGLSCFADHAVVPRNSVQ
                     KIDKAVPMHDAAMFGCAVVTGVGAAVNTAGTKPGDVVAVVGLGGVGLSCLLGAIVAGA
                     ERIIAVDLSDDKLALARQLGATETFNARDPGVVEAIVKATDGGVDTAFEMAGSVKAME
                     LAYKITVRGGQTISAGLPPVTGLFSLQHYGMVQDARVVKGSYMGGCVIARDMPRYIEL
                     YKRQKLPVDRLRSAFIRLDEVNEGFDRLADAQVVRQIIVFD"
     gene            27465..29816
                     /locus_tag="PK264_03100"
     CDS             27465..29816
                     /locus_tag="PK264_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74915.1"
                     /translation="MEPEPALGRFGSPGPTMPQVPSHAPGRGRRTGTHYPGTGCPLRR
                     HCPGVRSLDCSSRQGWLGDFSNRLFSQGGAMHSLLGALAVLRAPIVVAVIAYLVLLLP
                     GQSEQIFITISEMTLWTRPGRLVATVFRVLPAAILLGAVFLICARRLIRAVPAYRLVA
                     AKRHAELLDAISLVLAPVPITLLLVAYVRAVTEWPKSVGLIVPAAAFGAVAYLTSRNR
                     ARRHLTLVADRMCAMVGETSVGRVLMRVSIVIMTLNVLIASWPVQFGSLLGPIAVVFL
                     FLAVLCVAASLLTHVYDRFQVPAIPALAVLALVAGTYGLNHNHELRVLSKEPPPAAPA
                     LADAFNKWLENRPDIDDFEGRPYPVYLVSAEGGGLYAAIHAAATLAAIQDNCPAFAAH
                     VFAISAVSGGALGAAVFNSIVRAKPPPRNLPKDARCPPPQGDNNERQKLVREFFDRDF
                     LTPVMAAGLFPDFFQHFLPWPIGAFDRSRALEASFETAWREVMRDNPNPVRNYFSSDF
                     RSHWDAGADAPALLLNTTVVKSGGNILAAPFRVVSKWSDEGKMDVLLRTGTWSLAASV
                     GLSARFPIVTPPAWVKPHGTASFEVMDGGYFDNSGMWTIIDLAQQLLEAAKTGTGDKL
                     AAGSGQEVCAPGNLGVLEVRGRRQPFCVKLLKISAWADPSDRSVQGELAAPMTAFFSA
                     RVAHTHLAGIVAKRQYCGDAKCGLGQKSLQPHVYFRRLKLSRRLPLGWYYSRETVKGL
                     IASTAPRAACSDTVAPDSHDEQLKEIEMENSCLFSRIAADLRL"
     gene            29839..>30148
                     /locus_tag="PK264_03105"
     CDS             29839..>30148
                     /locus_tag="PK264_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74916.1"
                     /translation="MLAIRSAFSLAFLIAGSISMLTQLSTEARGQSRCESRSSASCTK
                     KGESFSCSGITYPKEFYCRCYLTDDGCVEDNLPSHIFRLDTATDAVKELQKKLGVKKV
                     T"
BASE COUNT         5117 a         9854 c         9546 g         5065 t
ORIGIN      
        1 ccgctgccgc cgctgatcgg cggcgggggt tcgtgcgata gcagtcgcct atcacacagt
       61 cgtgaacgaa tgcaactgct gtgccgtttg cctgtcatgc gccggcgaga cgtgcattcg
      121 ctcgctcctt cagttcggcc gtcgccgggc gattgtcaat cgccggaccc tgctatttct
      181 tgaccgtcca cgcctttgcg gcggccgcga cagcgttggc gtcgaacgtg ttcacgtcgg
      241 cgagcaattc atccgtcagg aagtccgccg cgttcgcctt caccttgatg atgccgttgg
      301 cgagcagcca ctcgagatag gcctggtagt tggcgacgag gtgctcgccc cacttcttgg
      361 cgccgacctt ctcgaggctc caggccggcg cgcgcgccat cagcgtcgcg atgtcgtggc
      421 ggagtgccgt cgcctcatcc ttgccggtcg cccgcgtctg cggccagatc tcccacatga
      481 tgcggatcgc ggcttccgga ttggtcacgg cgaaatgcgt gcccatggcg tagcccttgg
      541 cgagagcgac cgcctgcttt cgcttggcgg cgatggtctc ctcgagggcg aggaacgcgt
      601 tcgacgggaa cttgtcgatc gagggatgcg agaggcgccg cagtttgaac ccggcgtttt
      661 cgacgagggc atactgcgtg tcgaactgcg acagcacgtc gaccgagccg ttcttcagga
      721 gcgccgccgt ctggcccgcc tcgcctgcga cgacgatggt gatgtccttt tcgggatcga
      781 gccccgcttc cttggcgagc gcgcgggcga tgatggcgct ggccgaggcc atcgagatca
      841 caccgacctt cttgcctttc atgtcggcga cggtcttgta ggcgctgtcg gcgggaacgg
      901 ccatgccgta cacgttgccc tgataggccg tgtagaagaa cttgagtttg gcgccttggg
      961 cgcgcagcac cgcgaccggc tcgacggagg gcagcgagaa cgggatctcc ttggtcgcaa
     1021 cgaacttcac gcattcgccc gagcccggca gcggcaccag ctcgaccttg atgccctcct
     1081 tctcgaacca acccatcttg agtgcgatcg cgaagggcgc ggcggccgag ctcacggtct
     1141 tggcgcacca gccgacccgg accgtctcga cctgggcgct ggccgacggc gcggtcgcga
     1201 ccgctgtcac cgccgtggcg ccgacggcgg ctagccacat catcttcttc atacgctacc
     1261 tcccttggaa gacttcgatt gtccctgttc ggccgcctgc gccggatccc agaagaggag
     1321 gcgacggcgg atggcaccga ccgtcccgtt gaggatgagg ccgaccatcg acaggatgag
     1381 caggatcgaa aactggccgg cgacgtcgac gttcatgttc atgctctgga gcaacatgcc
     1441 gaggccgcgc tcggcgccga tgaactctgc gacgatggcg ccgatgaggg caaagatcat
     1501 ggcgatctcg agccccgcga agatgtacgg aagcgcgttc ggcagttgca gcatccagaa
     1561 gatctgccac ttgttggcgg cgagcgagcg catcaggttg atgcggtcct cctctgccga
     1621 gcgcaagccc acgatggtgt tgaccatcag cgggaagaag gcgacgagcg ccgcgttgat
     1681 caccttggat ttgaggccga gcccgaacca gacgatgatc aggggcgcca gcgccacctt
     1741 cggcatcgcc tggaacatga cgatgaaggg gtagaggaaa tattcgacgc gccggctcag
     1801 cgccaccagg atgccgaggc tgaaggcgag cacgcaggcg agcaggaagc cgagcatcgt
     1861 ctcgagcaat gtgacgccga ggtgctcggg atagatgttg cgcgcgatcc cctcgtagag
     1921 tgcgcggaac acctggctcg gcggcggcag gatgtaggcc tcgatggcga acaccttgac
     1981 cagcaactcc caggcaccga gcacggcggc gatcagcagg aggcggagag agaagtgctt
     2041 gacgccaccc atcagtcgag ccctccgtcg gcgttgagcg ccgtcctgat gcgccgcaca
     2101 tagccgccga acagctcggt gttcatcacc gagagcttgc gcgggcgcgg cagctcgact
     2161 ttgaaggcat cgccgatctt ccctggccgc gaggtcatga cgatgacgcg gtcggcgaga
     2221 tagacggctt cggaggtcga atgcgtgatg aagagcacgg tcttgcggct ctccatccag
     2281 atccgctgca gctcaacgtt catcgtctcg cgggtcatgg cgtcgagcgc gccgaacggc
     2341 tcgtccatca gcaggatggc cgggtcgtgg atcagcgccc ggatgatgcc gacgcgctgc
     2401 tgcatgccgc ccgaaagctc gcgcggatag cggttctcga agccctcgag gccgacgagg
     2461 cgcacgagat ccagggcgcg ggcgcgcatc gccttcttgt cgaggccctg cacgtcggca
     2521 ggcagcagca cgttgccgag tacgctccgc cagggaaaga gcaccggact ctggaagacg
     2581 acgccgatgt cgcgacgcgc gccacggatt ggcgtgccct tgagcatcgc ctcgccgctc
     2641 gtcggcggca gcaggccggc gagaatcttg agcagtgtgg actttccaca cccggagggc
     2701 ccgacgatcg agatgaactc gccatcagcg atcgagaacg agatgtccga gagcgcgagc
     2761 acggcgtcgg cattgcgacc gtaggccagc gacaattgat cgaccgagat gaccgcgggg
     2821 cccgaggccg ccgtcgcaat cgttcccagg tccgcgccag attgcacgcc ctggccgctg
     2881 acacccgtgc cgtccacgct gttcctcctc gaagctgcga ggactgacgc ctcgcttggc
     2941 ccttactgtg agggacgatt ccgcggctgt ccagcctaag gcttcgatcg atcgcaaccg
     3001 ccagccgtca tcgaccgttg caaatgcaaa ggagccgcca gcggcagccc ccgagatcgt
     3061 catcggatgc ctgtccgatc acgacgccat cagaacttga tgcgcgttcc cagaacgacg
     3121 gccgagaaat cattggcggc cacggactcg aaaacgggcg ccgccagcgt gccggcgttg
     3181 cgcgtgaagg tcgcgtcatg gtgcttgtcg gcgatgaaca gctccgaggc ggcggcatcg
     3241 atgtgctgca ccatgcccag gccccagaac atcacgtcgc tgctcacgac atcactgaag
     3301 cggtggcgca taccgtcggt gtgaggcgcg tactcgccgt cgaacacggt gcggccgatg
     3361 ccgaggtcgt tcttcgagat accgccgtcg aggtgccaga aggtcgaatc cggctcgcga
     3421 ttgtcccagg cgccgatcag ctctcgatgg ttctcgtcct tgcgccaccc gcctaccccc
     3481 gtgaggaaga ggccggagga cacatgaagc acagaggtcg agcccatcac gaccctctgc
     3541 agccgttcgc actgcgatgt gcacagcacg ccagccgcat tgaaaagctg gttcgtccag
     3601 ttgaactcgc tatcctcccg atagccgatg cccgaggcga tcctgaagcc gccgagatcg
     3661 gcagcacgcg cgccagttgc gcagagaccg aagtttgcat cgacgccatt cgtggagccc
     3721 gaccagagcg cccttccagg cggcgtggcg gagctacgtg acccatcccg ccgtgaagcc
     3781 gttcaattcg ccgaacatcc gcaccaacgc gatgccctgg ttgcgccggg ccaaccgtcg
     3841 aagccgctgc ttcgcgcggg tgaggccttt cggtgcgaga ccgatcctcg ccactgctca
     3901 ggtagcgatg actgagaaag gagcgttcct cgtcgggtgc gaccgcgctc ttcttgtctt
     3961 tgacctccag ccggaggtgg ccttcgatga aggccgtgac gtacgccatc acccgcttgc
     4021 ccgccgcctt cgaccgcacg tagatgatgc agtcgtcggc gtagcggcag aagcgatggc
     4081 cccgctgttc caagtcctgg tcgagatcgt cgagcagcag ctctgcgagc accggcgaga
     4141 gcgggccacc ttctggcgta ccctcgtgcc gcccgatcac cacaccaccc tgcatcatcc
     4201 ccgcattgag gacggcgcga acgatcttca ggaagcgctt ttcgccgata cgtcgcgcca
     4261 agcgggccat caccacatcg tggttgaccc ggtttcgcga tagggacggc cacctcctga
     4321 ccaagcggca tcacagacgc acacggcccc tgacgagcgc ccacgcgctc tccggggaac
     4381 cagcgggcga cggactacct tgcctaccgc ttcccgagcg tgccggctgc ctcgaggcgt
     4441 tcaatctccg ctaccccgat accaatctcg gcgagcacct cgcggccatg ctcgccgatc
     4501 cccggaaggc cgcggcggac gccgagccgg cgaccgtcga gctcgatggg cagcgccggc
     4561 gtcacggtct cgcgcccgtc cggcaaggtg atcggcaaga gcccgcccga tgcggtcaga
     4621 tgcgggtcat cgaacagctg ctcggggcgc acgatcggcg cataggggag accggccttc
     4681 gccacgacag cctcgagatc ggccaggcgc cacgacgcga attgctcgcg cagccgaggc
     4741 atgaactcgt cacgccggcc gcagcgcttc acgttggtcg caaggcccgg gtccgcatac
     4801 atgtccgcga gttcgaaagc ctcgcagaag agcttccatt gcgtgtccgt gacgacgccg
     4861 atgaagagct gcccatccgc gcaatcgaac acgtcgtaga tgccccacgc cgcaagccgc
     4921 tcgggcatgg gcggcgccgg ctttccggag accgcatact gcatcatgtg ctgggccgag
     4981 agcagcgcgc agctctcgaa gagcgcgctc acgaccttct ggccagcccc cgtccggtcg
     5041 cgctcgcgca acgccgccag aatggcgatc gcggcgaaca tgccgcccat gatgtcattg
     5101 acagaggagc ccgcgcgcag cggccgcccg cgcggtccgg tcatgtaggc gaggccagcc
     5161 atcatctgga cgacctcgtc gagggcggcg cgctcctgat agggacccgt gaggaaaccc
     5221 ttgagcgagc agtagatgag ccgcgggttg accgtcttgc acacgtcgta gccgagcccc
     5281 atggcatcga gcgcgcctgg ccggaagttc tcggtgacga cgtcggcgga ggcaatcagt
     5341 cggcgcgcca cctcgaggcc ctccggcgac ttcagatcga gcgcgatcga gcgcttgttg
     5401 cgattgaagg ttgcgaagaa gccgatccct gaaccgatca gccggcgcgt attgtcgccg
     5461 tcgggcgccg gctcgaactt gatcacatcg gcgccgagat cgccgaggat catgccgcag
     5521 gtcggcccca tcacgacatg gcagagctcg atcaccttga tgccgtcgag cggcagtgcg
     5581 cgttggctca tgcgaaccag gcctcccagt ttgttgcgcg gctttacctg tccttaaggt
     5641 cgccggcata gtgttcacgg cgtaccccaa tcctgttcac gcgtctcggg tgttgcgtat
     5701 cgtggtctat ccagatcggc atcgacgtcg cttgggacgg ctcccagtgt tgctcatctg
     5761 tctcgggctc acggcatcgt tcgcctatca cataagacac ggccggcatg gtctcgaagc
     5821 tcgttcgacc ctcctcgcac gctccgctgc attggagctg agggtccacg cgctcgagac
     5881 cgtgcgggct cgtcttgcac gcgacatcgc gctcctcggc gcggcacccg atcgcgacct
     5941 cgtcagcgag cttgcgcagg agctggtaga tctcctgctt ggtgccgaca tnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     6121 agctcgaggt tgtgggtggc gatgagcgcc gcgacgcccg tccgctcgat gaggccgatg
     6181 agctcgcgga acacaccttc ggacgtggta ggatcgagat tgccggtcgg ctcgtcggcg
     6241 agcacgatgc gcggcgcgtt ggcgagagcg cggcagatcg ccgttctctg ctgctcgcca
     6301 cctgaaagct gtccaggccg gtggtcgagg cgctcgnnnn nnnnnnccca tgagcgtcag
     6361 cagctcgccc gcccgctcgg ccgcgatgcc gcgagctttc cctgcgatca tctgcgggat
     6421 catgacgttt tcacgtgcgc tgaactcggc gagaagctgg tggaactggt agatgaagcc
     6481 gatcgtcgag cggcgcatgg ccgtgcgcgc tccgtcatcg agatcggcgc aatcccggcc
     6541 gccaacgatt acctgccctg ccgtcggccg ctcgaggagg cctgcgacat ggaggagcga
     6601 cgacttcccc gagcccgacg gcccgacgag cgccaccgcc tgcccgggct cgagcttggc
     6661 cgaggcgccg accagcacat ggaggtcgcg ctcggcctgc cgaaaggtgc gcctgatatc
     6721 gacgagctcg aggattgcca ttgccccctc cctcactcgt acctgagggc ttcgatcgga
     6781 tcgaggcgcg aggcgcgcca ggcggggatg agggctgcga gcacggccat ggtgatggcg
     6841 aagatcacgc tccgcgtcgc ctcgccgaca tcgatgaggg ctggcatgcg cgtcaggtag
     6901 tagattttcg gattcatgag cacgacccct gtcgcccact gcgcgaacag gcggatctgg
     6961 tcgatgtagt ggcagaacac gatcccgagg agcagccctg cgatcgtccc gacgacgccg
     7021 atcgtcgcgc ccgcaatgag gaagatgcgc atgaccgacc atttggtggc gcccatcgtg
     7081 cgcaggacgg cgatgtcacg gctcttgctt cgcacgagca tcgtcagggc ggagacgatg
     7141 ttgagggccg ccacgaccac caccagcgag acgatgatga acatcatcgt gcgctcgacc
     7201 tcgagcacgg tgaaaaattc cgagttctgc cgccgccagt cggcgagatg caggctcggc
     7261 cccgccgcca tctcgatgaa cggaagaacc ttgtccatgg cctgcgggtt atcgaccgtc
     7321 acctcgatgg catcgatgtt ggcgcccttg cggaaatagc gctgcgcctc gccgagcggc
     7381 atgaagatga ccgtgcggtc gtactccgac atgcccatct cgaagagcgc ggtcaccgtg
     7441 tagcgcttgt tcgcgggcac ggtgccgaac ggcgtctgcg cgccgcgcgg cgcgatgagt
     7501 gtcacgccat cgccgatggt gacaccgaga tcacgggcga gccgcgtgcc gatgacgata
     7561 ccctcgccgc tgtcgaagtt gtcgagcgag ccgaacttga tgttcttgcc gaccgagggg
     7621 aggatcttgg cattggcctc gctcatgccg cgcacgaagc cgccgagcgt ctgcgattgc
     7681 gaagcgatga tcacctgccc gccgaccagc ggcaccacgg acttgacgcc aggcacgccc
     7741 gtgagccgct tcacggcgtc ggcgtgatcg tcaaaaggcg caccgatctt ctggatgacg
     7801 acatggccgc tgaggccgag gatcttctcg agcagttcct gacggaagcc gttcatcacc
     7861 gccatgacga tgatgagcgc agctacgccg agcatgacgc cgaggaacga gaggaaagtg
     7921 cgcagcggcg gcatccagcg ccaggtcggg cgcggcagca gatagcggat cgccatgcgc
     7981 cattcgaccg gcgcgaacat gcgggccgca gcgagcgggg ccgcctcggc aggttggctc
     8041 gcggcagcag aggcggtggc gtgatgtccg gccgtcatga gcccaggttt ctccccactg
     8101 tggagccggc ggcgacaagc gagcccgaca ccggctcacc gtcccttcac gtcacactgg
     8161 cagccgattg tggcaagcag atcaagcgag cacgcgctgc gacgtgacgt ccgcgatcac
     8221 ggccgagagc gccgcgtcta tcggcaaggt gcgccgcgcg ccgcccttgc gcgccttgat
     8281 ctcggcctcg cccgccttga cgccttttgg tccgatgatc agctggtgcg gcacgccgat
     8341 cagatccatg gtcgcgaact tgactcccgc ccgctcgtcc gtatcgtcgt agaggacatc
     8401 gatgccggcg ttcgtcagct tctcgtagag gctcccgcag acgcgctcgg tatcggcatc
     8461 gcccgccttc aggttgacga gcgccacctc gaacggtgcg accggcaccg gccagatgat
     8521 gccgtccttg tcgtggtgcg cctcgatgat ggcgccggcg agccgcgaga cgccgacgcc
     8581 gtaggacccc atctgcacgt tgacgagctt gccgtccgag ccctgaacgc ggcagcccat
     8641 cggcttcgag tacttgtcgc cgaagtagaa gatgtgaccg acctcgatgc cgcgcgcggt
     8701 cacctgcttg tccttcgcca cgtcgcgatc gaaggctgcc gcatcgtgct tctcgtcggt
     8761 cgcggcatac ttcgacgtcc acgccttgat gatcggcgag aggtctccgt tgaagtcggt
     8821 gccggcaggc gggatcgccc cctcgatcag gtccttgtga caatagacct ggctctcgcc
     8881 cgtatcagca agaatgatga actcgtgact gagatcgccg ccgatcggcc ccgtatcggc
     8941 cgccatcgga atggagcgca gccccatgcg cgcataggtg cgcaggtagg cgacgaacat
     9001 ccggttgtag gaggcgcgtg cgccctcctc cgtcaggtcg aaggagtagg cgtccttcat
     9061 caggaattcg cggccgcgca tcacgccgaa acgggggcgt acctcgtccc ggaacttcca
     9121 ctggatgtgg tagaggttgc gcggcaggtc cttgtaggag cgcacgccgt cgcggaagat
     9181 ctcggtaatc tgctcctcgt tggtcggacc atagagcatc tcgcggtcat ggcgatccgt
     9241 gaagcggagc atctcgggac cgtacgagtt gtgacggccc gactgacgcc agagatccgc
     9301 tgactgcacc gtcggcatca gcacctcgat ggcgcccgca cggttctgct cctcgcgcac
     9361 gatctgttcg atttttttca gcaccttgaa cccagtcggc agccaggaat agatgccggc
     9421 cgcagcctgt ttgatcatgc ccgtgcgcag catgagctgg tgcgagacga tctcggcctc
     9481 cttcggcgtc tcgcgcagca cgggcatgaa gtagcgggag aggcgcatgg ctcggatcct
     9541 cgtcgactgt gaggcgggta ggcgcgggcg cacgcgttca gcgcgctgca gtcttcaaag
     9601 tgcgtgcttg atagccatta agatcgggcc gtgcaatgga ggccgcaggc gcctgctcag
     9661 gcgatgatgt caggggtcag gcgatcctcg atggccgtga tctggtcctt gagcttcagc
     9721 ttgcggcgct tcaggcggga aatctgcaac tgatcgctgg tcgaactcgt ctcgagcacg
     9781 acgatctcgg catcgaggtc gcggtgctcg gcgcgcagcc gcagcagcaa ctcgcgcagc
     9841 tcccgatcat cggcccggtt catccgctcg catctcccga tcgaccctga ttcgagccgc
     9901 gcccattatc gggcggcgac cgccgcgagg caagcatgcc gctcgtacaa gagactccgc
     9961 gagcgccaag gcggcctcaa ggctatgcaa gtttccgtat cagagctgcg tgggcggcac
    10021 cgggctgcta tcacccatgg aaatcgtcat tgtccgcacg ccgaaggaga ccctcgtgac
    10081 caagaagccg atcctcatcc tcaatggccc gaacctcaac atgctcggca cgcgcgagcc
    10141 gcacatctac ggggcgacga cgctcatgga ggtgaaggcg atgtgcgagg cgcgggccaa
    10201 ggcacgtggg catgcgatct cgttccacca gtcgaacaag gagtatgagc tcgtcgaatg
    10261 gatccagggc tcgatctcga ctgcggcggg catcatcgtc aaccccgcgg cattcacgca
    10321 tacctcggtt gccattctcg atgccctcag catgttcaag gggccgatca tcgagttgca
    10381 catctcgaac ccgcacaagc gcgagagctt ccgccaccat tcgtacgtga cgcaggcggc
    10441 aacggccctg atctgcggcc tcggcgtgaa gggctacccg ctcgcggtcg acgccatggc
    10501 cgatctcctc gaggccgcga gcgcccccaa ggcctgagcg ggcctgcacg gatctcgtcc
    10561 gcccggcgac cttgcgaccc ttgaccgagc ccgcgtgagc ccgcatcagt aggctgcaaa
    10621 ccgagccttc gaggagcaga ctggccatgt tcgacttcga tcgcgtcatc gagcgccgcg
    10681 gcacccatgc gtcgaagtgg gacaacatgg caaaactctc cggcatcacg gcgcccgatg
    10741 cgatcccgat gtgggtggcc gacatggact ttccggcgcc gcccggcgtg acggcggtgc
    10801 tcgccggcga ggtcgggcgc gcaacgcacg gctactacgc cgacaccggc tcatggaccg
    10861 agtccatggt cgaatggatg nnnnnnnnnn nntcaaggtc gatccgtcgt gggtgagcgc
    10921 cacgcccggc atcgtctcgg ggctcggcct catcatccag gcgctgagcg agcccggcga
    10981 cgacatcgtc gtcttcccgc ccgcctacca cgccttccgc aagatcatcc tcgccaacga
    11041 gcggcgcatc ctcgatgtcg gcctcgtcga acgcaacggc cgctacgcga tggacctcga
    11101 tctcctgcgc tcgcgcctca cgccgcgcac caaggccgtg ttcctgtgca gcccgcacaa
    11161 tccgggcggc acggtctggt caccaaacga gctccgcgat ctcgccaagg tctgcgcgga
    11221 gcgcggcgtc gtgctggtct cggacgaaat ccattgtgac ctcgtcttcc caggcgcgcg
    11281 ccatacgccg accatcgccg ccgcccccga gctcgccgag aacatcatca cttgcgtcgc
    11341 ggcgacgaag acgttcaatc tcgccggcgc acacgtcggc gcctgcttca cgtccaaccg
    11401 caccttgaag aagcggctcg acgcgcgtat cgcggcgagc ggcctcgcct cctacaacag
    11461 cttcggcatg ctcgccacgg aagccggatg gcggagcggc gaagcttggc tcgacgcgct
    11521 catcccttat ctcaaggcca atcgcgacga gttcgaccgg cgcatcgaga aggccgctcc
    11581 cggcgcgcgc tccatgcggc tcgactcgac gtatctcgcc tgggtcgatt ttgcgaagac
    11641 cgggctcgcg cccgccgacg tcgccgagcg cgtcgccaag cgcgcgcgca tctatgcgag
    11701 ccccggcgag cagttcggcc cgggcggagc gaactcgctg cgcttcaact ttgcaacccc
    11761 gcgaccgatc ctcaacgagg cacttgcgcg cctcgaggag gcgttccgcg atatccggac
    11821 cggcgcctga gcggagagtt gtcatgcggc ctgtgatcgg ggtcatcggc aacacgcacg
    11881 tcatcgagaa ccgctttccg gcgcaactcg ccggcgagcg caacctccgc gccatcgccg
    11941 aagtctgcga ggccctgccg ctcgtcttcg ccggctgccc cgaaatcacc gacatcggct
    12001 cgctgctcga gacggtcgac ggcatcctct tgaccggcgc gcgcgccaac gtccatccga
    12061 cgcgcttcag gacggagccc aatcccaagc acgagcccta tgatgagcgc cgcgatgcca
    12121 tggcgctcgc cgtcatcaac gcctgcgtcg cgcggtcgat ccccgtgttc ggcatctgcc
    12181 gcggcttcca ggagatgaac gtcgccttcg gcggctcgct gcatcccgag atccgagaga
    12241 ttccgggccg catgaaccat cgcatgccac ggctgccgac gggcgagatc caccccgacc
    12301 agacagtcgt gttcgccgac cgccacgacg tccgcctgaa acctggcggc catttcgcga
    12361 cgatcctcgg cacggattgc atccgcgtga actcgctgca cggccagggc atcctggagc
    12421 ctggtcagcg ggtcgtcatc gagggtgtcg ccgaggacgg cacggccgag gcgatctgca
    12481 tcgagggcgc cgccggattc gccctcggcg tgcagtggca tgccgagtac gacccgcagc
    12541 gcaaccccgt caaccgggca cttttcaagg cgttcggcga cgccgtgaag agctaccgcc
    12601 acgcgtcctg acgcggctcc gccttacgaa cgccgaactt catgacattg gtgtgacagt
    12661 tgccggacag acggcgacgc tcacgcagtg gcatccaata tggccacgtg tgcagcttgc
    12721 ccgcacaccg gaagaccagc cagcaatgtc cctcagcgtc gaagcggccc ccgcgcaccg
    12781 ccggctcggc tcgtggttcc tcattgcgtt tccccatctg ggtgcgcttc tcatcctgct
    12841 cgccaccgag acgagcctcg tcggccgctt tgccttcgtg ctcgcctggg cattcttcac
    12901 ccttggctgg atggccgtgc tgcggcgacc ggccgtcgcg ggtatgctgg cgctcggctt
    12961 cctcgtgctc ctcgtgctgc tctcgaggtt caagcacgag atcctgctga tgaccgtgaa
    13021 cttcgtcgat gtgatgctga tcgacgccga tacgattgca ttcctgctgc aggtcttccc
    13081 gcgcctcgat ctcaaggcgg cggccggagc gatcgtggtg gctgcggcct tggcgatggt
    13141 gtggcgcctc gacagggccc gcgtccggcg ctcgctcgcg gcgggcggtg ctgctgcctc
    13201 gctcggcggg ctggtctgcc tttcggtgct cgtcccgaac gatccctggg aggagttcta
    13261 cgccgagaac tacttctcga aatacgcccg ctcggccgtg accgcgatca gcgatctcat
    13321 cacgaggggc gtgctcgaca gcgacaagac cgcaaccgag cggctggccg cgagcccaag
    13381 cacgccgtgc aggctcgata agccgccgca catcatcatg gtgcatgacg aatcgagctt
    13441 cgatgcgcgc gccattcccg gcctcaaggt gccgaagggc tatggcgatc acttcgtctc
    13501 gggcgacgga aagcgccgga cactgctcgt cgagggcccg ggcgggccaa gctggtacac
    13561 tgaatactcg atcctcaccg ggctatccgc acgctcgttc ggccgcttcg tcgatttcac
    13621 aacgcgcatc gccgtcggtc ggatcgagcg gggcctcccc cgcgcgcttg cccgctgcgg
    13681 ctataagaca ttcacgctct acccaatgta tggaacgttc ctcagcgccc gcgccttcca
    13741 gacgacggcc ggaattcaat ctttcatcga cgcccaatcg ctcggcgcca agttccagga
    13801 tgccgacagc ttctatttcg acgccgccgc cgcgaccctg gcgaagcacc gggccgaggg
    13861 gcccctgttc gtccttgtct atacggctca gaaccacttt ccctggacat ttcgcttccg
    13921 cccggacctc gctcccgact ggacggcgcc aggcaatcgc gccgacgtcg acgagtacct
    13981 ccgccgccag cacctgaccg cggtcgatta ccaggcattt ctcgcgcgcc tgaagcgcga
    14041 gtttcctgga gagcccttcc tgatcgtgcg cttcggcgat caccagccgt atttcnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccct cgacacgctc
    14221 gacgtcccct atctggcgct cgtcgtgctc gaggccgcgg gcgttccgct cgatccctcg
    14281 ttcactgagc agaagctcat cctcgagcgt tgccgcggca tatttcaccg ctgcaacggc
    14341 ggcgcggagg cgcgccgctt caaccgcctc ctgatcgatg ccggcctgat caaggggctt
    14401 tagtccgagc gcgccgcggc agttcggcgc gcgtgcgccc ggtgatcttc aatgcgaagg
    14461 cgtagcgcat cgcctcgtcg cggagccgct cgaagcgccc gggcggtcca ccatgcccgc
    14521 cgaaattcgt gtggagcagc aacaggttcc gattggtgcg catggcccgg agccgcgcga
    14581 cccacttggc cggctcccag taggcgacac gccggtccga gatggcgccg gttgccagaa
    14641 tgtgcgggta gggctgtgcg cggacgttgt cgtacggcga gtacgatcgg atcagatcga
    14701 aggccgactt gctcgcctcc ggattgcccc actcggggta ctcgagttgc gtgagcggct
    14761 ggctcgcatc gagcatcgtc gccagcacat cgacgaacgg cacgtcggca atcacgccga
    14821 ggaagagctc cggcctggca ttggcgaccg ccccgacgag catgccgccg gccgatgcgc
    14881 ccgcgatcac gatgttgccg cgcttcacat ggccttcggc aatgagatgc tcggcagcag
    14941 cgatgaaatc cgagaacgtg ttcggcttct tctcgccccg cccctcgaca taccagcgct
    15001 cgccgcggtc gttgccgccg cgcacgtgcg cgatcgccat cgtgaagccg cggtcgacga
    15061 gggagaggat gtcgcgctcg aagcggggtt tggacgccac gccataggcc ccataacccg
    15121 tgaggtagag cggcgcgtcg ggatcgccgg cacggtcggc ccggtggaag aaggtgatcg
    15181 gcaccagctc gccgtcggcg gccttggcca tgagacggcg ggtgacatag tcgtccggcc
    15241 ggtgaccgct cggaatgcgg gtctccttga tgacgacccg cttctcggtg gtcacatcgt
    15301 gcttccagta gcggaccggc gtcgtcggcg cctcgaacga gacgatgagc tcggtcgacg
    15361 actgctcgta gcccgaatgg ataccgagca cgtgcggccc atcgccgaac gagacggcat
    15421 gctcgcggcc gtcgctcagg cggcgcacca tgatgcgcgg cagcccgtcg gtctcctcga
    15481 gccagatgag ctggtcgtcg agcgtctcga gccaggtgat gacgcggccc ggcacatgcg
    15541 gcacgagttc ccgcggaggc gaggtctcga agctcgcgag aggcgcctcg atgatcttgt
    15601 tgtcgagagc gccatccgca ttggtgagcg tgatcaggcg ctcgccgacg atctcgacgc
    15661 tgtagcggat attgtcgcgg cgcgcggcga ccattcgcgg gggcgcctcg ggtgctcgcg
    15721 catcgacgag ccgaatctcg ctggtctggt gtgtccccga ctcgatcgcg atatagcgcc
    15781 cggaggccag ccgcgacaca tcgacgtaaa agccgagatc cgtctccttg tacaccagga
    15841 tgtccgcgct ggggctggtg ccgagacggt ggcggtagac gctcaagggc cgcgagtcgt
    15901 cgtcgagacg cacgtagtag agccactggc cgtcgagcga ccagaccgcc cggccgttcg
    15961 tgttcgggat gctgtcggga aggtcgcgac gtgatctgag gtcgcgcacg cggatcgtgt
    16021 agctttccga gccgacggga tcgacggcat agacgatacg cgtgtgatcg gggctcggca
    16081 gccacttatc gaagaggctg aactccttgg agccggccgc cagccggtcg ccatcgacga
    16141 gcacctcttc tgcgccgccg gcgagcggcc ggcgcaccat cttgaagcgc tggccgccct
    16201 tgacggtgcg gcggtagtag ctccaggcgc cataggtcag cggtacggag gtatcggcct
    16261 cgacgatgcg ccccctcatc tcagcataga gttttgcctg gagcgccctg gtctcggcca
    16321 aggtcgcggc cgtatggcgg ttctcggcct cgagatgatc gcggatgcgc ttctcgagct
    16381 tcgtcggatc ggaacgcacc tcgttccagt tcctggcagc gagccaatgg tactcgtcga
    16441 cccagctcgt gccgtggagc gagaaggtct tcggctcgcg cagcgcggtc ggtccggcag
    16501 aatcggactt cagtgcaggg gattgtccgc gggcttcaag cgcgctccaa gtcgccacga
    16561 ttgcggcgag gagcgcgggt ataatcgacc acggagcaca acgacggatt ggcattgtga
    16621 cctcggctca atggatatcg aacatgcatc caatagcagg gcatcacaac acgaacggct
    16681 cgccgagcgc catatgaatt cgccttgacc tcctactggc agcggcgctc gctgactggc
    16741 ttccagctcc gactcatgcg ctcgacgaca ctgtcgtagg tcgcgcgctc ggcggcgggg
    16801 tagaggatct gccagccgga aatgatgcgg ccgcggcagc cgaactcgac gcgctcgtag
    16861 aaaatctggt cgcccttgaa gcccgagacg atgaagagac ggttgtcgcg gcggccatag
    16921 ctgaggcggg cctctggata ggccgtcctg aggacgaacg cccgatagct ctcgaccgtt
    16981 tcgttggtgg agttgacgct cgccgaaacc agcagcttgg ccttgccgtc aactgatacg
    17041 aagagctggc cgtcgggccg tcccggtccg gccgccttga agatctcgcc cggatagctg
    17101 atcgtaaacc cgtagcggtc gttctggtag acgggccacg cacgcgtctg cgcagcggtc
    17161 ccccccgcag caccgagaag cccgagcagg ctcgagaggc aacttatcgc aaggcgacgc
    17221 tgtgacatgg ccaatctcaa ggtgcggcga ccacccactg atcgcgcagc cgccattcag
    17281 acgattccgt atcacgccga tcaaatgacg attgagccgg atgttatctg attttcatat
    17341 atcactttat ttgccgcgac cggtgccgat ggggccggcg gctggcgacc agccgagcca
    17401 gccgcgcgag cgcgtcgacc tgaggctctg cacctgctcc cagggtacga actcgaccgt
    17461 cttgtcggtg cccgcgaaac cgaggagccc gcgattggcg gagacaataa tcgtgaacgg
    17521 ctcggcgcgg ccggcgcggg tgagcagcgt cggggcctca ccctgatgca gtgagacggc
    17581 acgtgcgatg ccgacgggga agaacgcgag cgtgccgaag atcaccatgg ccgtgatcga
    17641 gatgcgcgcc gtcagccagc aaccgacgca ggtcaccgca atgagcggtc ccgcaatcgc
    17701 cagaggcaga taccagacag ccaccgcggc gaatgcaagc gccatcggca tcacgttggt
    17761 ggcatgcttc cagacctcga gctcgggcat ctggtcctgc gcgaggaagc cgagcgcgcg
    17821 aaccaggacg acgatcgagg cgatggccac cgcatgcagg gccacggtct gaaagatgct
    17881 ggcatgatcg atcgcctcga agaggtcgat cccgatagcg gcgaaggttc cgccgagcag
    17941 gaagatgcta ctcgtcgtcg cgaacgagat gacgagtgcg aggcgcgcga ggaggccgaa
    18001 tacgcgccgc gtgatcccct cccagagccg ctccatgcgc tcggcgacag gctcgatcgt
    18061 gcggcgccgg atgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggccaccg
    18121 cgccggtcgc gctgctcacg gcatcggccg ccttccgccc gccctcccgg gcgagcaccg
    18181 ccgtttgcga ggtgaggccc atcaccttct gtcccgtcca gcggaacggt gagagaagcc
    18241 acgacacgaa gccggcgcgt gcttcgccgg caccggcaga cgcagcactg cctgcatcgc
    18301 cgcctttccc ttgaggtgcg ggacatcctg gctcgcttga cccgtgacca tcgctcttca
    18361 tcacacgggc tcgctggcaa tgatggcaaa gtcgtcgccc gtgtgccggc tcgagcccgg
    18421 cgccgaggca tcagtgagca gcagcgtcgt ccccggatgg aacatgcggg cgatgcgcag
    18481 cgcgcgctcc ggatcgctaa agacgacgcg cctcatcgcc gtatcgctga gattggtgac
    18541 gatgtgcgcc tcacggctcg acttgcctat gccgaaggcg agccaggaga gcgccttgcc
    18601 gtccgcagtg gggccgacca gcgaataggt atgggtgccg agcgggcgcg ccggcgactt
    18661 gaacgtgacg gggtagctcc cctcgacctt gccgtcggtg acgatgatcg ccttgccgtc
    18721 gcggcgcgag atcacgacat gggtggtcgg ataggtgacg gttgcgtccc aggcaccgtg
    18781 cgcacgccgc ttggcgacgt gctcggcgac gctcgtcgcc tcgacggcgg ccgcatcggg
    18841 cagcagcgcc gcgggatgca cgacactggc gggctgtgtc cgctcgtccg cgatgatcac
    18901 aacgacgccg agatgcgtca cgtcgaagag gagggccgaa aacttgaggg gcagcctgac
    18961 acaaccgtgc gaggccggat agcccgggag gttgccggca tgcagcgcga tgccgcccca
    19021 tgtcaaccgc tccatgttgg gcatctgcgc gcccttgtag atcgaggaga cgtgcttgcg
    19081 ctgcttctcg agaatggtga agacgcccgt gggcgtcttg tgtccgggac gccccgtcga
    19141 gcaggtcgag acgccgatct cgatgccgtt gcggtagatg tggacgagtt gctcgggcag
    19201 cgacacgatc attacaaccg gtccggaggg cgagcgctcg ggcgtccagg caaattcgcc
    19261 gggagccatc ttgcgtccgg ccgtgtagcg ggccgcttcg gaggacggga ccggcaagcc
    19321 tgcggccccc gcggcagaca atccaagccc gagcgccgcg cgtcgcgtga tccgggcggg
    19381 tgcgtcgtct ggtccgttcg agcgcatgtc acgttctccg cgttcgttcg acattcgtct
    19441 gaacgccggt cgtaccgcaa tgccatccat ccgaagcgcc caacgccgca tggcgtcggc
    19501 ggcgatccac tcgggcctac atccggaata cgccgaactt cgtcggctcg atcggcgcat
    19561 tgaggctcgc ggcgagcgac agtgcgagga cgcgccgcgt ttcagacggc gcaatgacgc
    19621 cgtcgtccca catgcgcgcc gtgccgtggt aggggtggcc atcgcgctca taagcctcga
    19681 gcaccggctt cttgaaggcc tgctcctcgg acttggacca gctcttgcct tcggcctcga
    19741 tattgtcgcg gcggacggtc gcgagcacgc tcgcggcctg ctcgccgccc atcaccgaga
    19801 cgcgcgcgtt gggccacgtg aacatgaagc gcggcccgta ggcgcgcccg cacatggcgt
    19861 agttgccggc gccgtacgag ccgccgatga cgacggtgag ttttggcacg ttggcgcagg
    19921 caacggccat caccatcttc gcgccatggc gggcgatgcc gcccgtctcc gcctcgcgcc
    19981 cgaccatgaa gccggtgatg ttctggagga agagcagcgg gatgcgccgc tggcagcaga
    20041 tctcgatgaa atgcgcggcc ttctgcgccg tctccgagta gagaatgccg ttgttggcga
    20101 ggatgccgac cggcatgccc tcgatccagg cgaaacccgt gacgagcgtc gtcgcgaaat
    20161 tgcgcttcca ctcgtcgagc tcggagccgt cgacgaggcg ggcgatgatc tcgcgggtgt
    20221 cgtactgggt cgtgagcgcg gccgggacaa tgccgtcgag ctcggcggga tcgtaggcgg
    20281 gaggccgcgg ctcgatcagg ttgacgtccg ggcgccgcgg cggcggcagg ctcttgacga
    20341 tggtccgcgc gagggccagc gcatggtcat cgttgagcgc gtaatggtcg gcaacgcccg
    20401 aggagcgcgt gtgcacttcg gcgccgccga ggtcctccgc cgacaccgtt tcgcccgtcg
    20461 cggccttcac gagcggtggg ccgccgagaa agatcgtgcc ctgccggcgc acgatgatgg
    20521 tttcgtcgga cattgccggc acataagcgc cgcccgcggt gcagctcccc atcaccacgg
    20581 cgatctgcgg cagccccgac gccgacatcc gcgcctggtt gtagaagatg cgcccgaagt
    20641 gctcccggtc cggaaagacc tctgtccagt gcggcaggtt cgcaccaccc gaatcgacga
    20701 gatagaggca cggaaggcgg ttctcgatgg cgatctcctg ggcacggaga tgcttcttca
    20761 ccgtcatcgg gtagtaggtg ccgcccttga tcgtcgcgtc gttgcaaacg atcacgcatt
    20821 cccgcccggc cacccgcccg acaccagtga tcacgcccgc gccgtggatg tcacccgtgt
    20881 acatgccatg ggccgcaagc tgcgagagct cgaggaaagg ggaacccgga tcgatgaggc
    20941 cgaggacgcg gtcgcggggc agcagcttgc cgcgcgcgac gtggcgctgc ttgagcttat
    21001 caggcccccc acctgtggcg tcccgccggt gctgatccag gtcgcgaacc agggcctccn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc gacgtgacgg cggcccccgc cggtagcggc
    21361 aaggtcagct ccggcggcgc gggcgcagtc ggcatcgcca aggccggtcg cggcgagacg
    21421 gcttgcggcg ggccgcgaaa ggcgagatag aggatgcggc cgacaaggct gaagaagccg
    21481 atgaccagca gaatgccgag aacgatcacg acgaggcgca tgcggcggat gaggcgctcc
    21541 tgccgctcgc ggtcggcggg actcatcgct ggcgcggcct ctggcgacgc ggtctcgctc
    21601 atcgctgcgg gtgtccgtcg gcgccccggc cgagccgagc agcctcgatt gccatagctc
    21661 ccaccataag cgacgctcca ggacgatgtc tcaaggtgcc gtctcccaca cggtgatgat
    21721 tgccgacgag caggcggggc tcaggctcga tcgcgcgctc gccgcagcct tgccggacct
    21781 cagccggcaa cgcctgcagg cgctcatgcg tgcgggcgct gtacgacgag cggggcggac
    21841 gatagatgac ccctcggcga gggtcaaacc tggcgacgtc tatacgatcg acgtaccgga
    21901 gcccgaggca gcgacacccg aggcggagag catcgccctc gacatccgct acgaggatga
    21961 ccacctcatc gtcatcgaca agcccgcggg cctcgtcgtg catcccggtg ccggtcatgc
    22021 gagcgggacc ctcgtcaacg cgctgatcgc gcactgcggc acgagcctct cagggatcgg
    22081 cggcgtcaag cggccgggca tcgtgcaccg cctcgacaag gatacgagcg gcctcatggt
    22141 ggtcgccaag agcgatgcgt cgcaccgtgg gttgcaggag cagttcgcaa gccacggcgc
    22201 cgacgggctt ctaacccgcg catacctcgc gctggtctgg gggcagccgc tgaggccgag
    22261 cggcgtcatc gatgcaagcc tccagcgcag ccgcgccgat cgcacccgca tcgccgtcac
    22321 taccagcgag gaggcccgcc atgccgtcac gcatttcaag gtgatcgagc ggtatgtcga
    22381 tgccaagggc aagccgctcg cgagcctcct ccgcctcgag ctcgagaccg ggcgcacgca
    22441 ccagatccgt gtccatctcg cccacatcag ccatcccgtc atgggcgatc ccgtctacgg
    22501 tgcgggctac aaggcgagtg ccggtcgcct gacacaggcc gccagggccg cgctcgaagc
    22561 actcggccga caagcgctgc atgcaacgca cctcgggttc gagcacccca tttccgggaa
    22621 atccatttcg ctcgacagcg cgctgccggt cgacattgcc cggctccgcg atctcttgag
    22681 aatgcccgac tgagattgct gctctaagct tatccccgcc gttcacaccg ttcataggat
    22741 atccgggcga acgcacccat ccggcggtac tctgaccgtc agctgccagc cgactgttcc
    22801 cccgtctccg cccccgagga gtccgatgcg ctctggtatg ctcgacctgc aactgaccgt
    22861 catgagaacc gacgcccctc acaatggccc agcgatccta ccgccggccg gccccgccaa
    22921 tttggccgcg atgccgattt ccctcatggc gggcgtggcc ccgcggcctc gcccaccgac
    22981 gatagagctc tccgatcccg acgcggcctc gctgctgccg ccacagcaga tcaaagccca
    23041 gatcgagcat tatgtcgccg tcgccttcgc cgtgacgctc gacgacttgc gccggccgtc
    23101 acgcggaccc gcggaggcgg cgttcgcccg acagatcgcc atgtatctcg cccatatcgc
    23161 ctgcggctgg accttcaccg aggtcgggcg gcttttcgag cgggatcgca cgacggtagc
    23221 gcacgcctgc gcgctcatcg aagacagccg cgacgacatc agcctcgatc gcgcgctcga
    23281 gtggctcgag cggctcgtgc gccggttggc gagcccgcgc cgcctggccc gcgagtggcg
    23341 atcggcgctc gacgacagcg actaggggag gcagttttca attgcgccgt accgtgatag
    23401 acaagtcact tttcaggact tggagaacgg ttatggcaat tggaaatgga cgacggtcaa
    23461 tgccgttgag tgccgtgtct ggaacgctta cgacattgac cctactgttc gtcatcgcgt
    23521 cgagtgtgtc gtctggattc actcaacaaa aatcgcgaac cgacacaaag agcgcgaact
    23581 cgagttcgat cgaatccgct gagacactcc tcaccagcgg caacgatgaa gccaagcgcg
    23641 gcaactacga ggctgcgatc aaggctttca aatcggcgca tgacgccttc aagaaggcgt
    23701 ccgagaaatc ctccaagaag gaggagccgc tctacaatat gacgtacgcc aacgagcgca
    23761 tcggcgacgt ccacctcgtg gcaggcaagc acgaggctgc gattgcaatc tactccgccg
    23821 tgctggtcga gcgcggtaag ctcgcgaaga cgttcccgaa gagttccctc atgatggccg
    23881 gtgtcggctt cgctcacgag cgcctcggaa ccgccgagtt cgaccggggc aacgacagcg
    23941 aggccctgag ccggttcaac aatttccatg acttgctgaa ggctctatcc gaaagcgata
    24001 gcaaggacat ccgctggcat cgcgggcact cgatctcgca caccaagctc ggcgccgtgt
    24061 atcgcaagca gcgcatcaac aagcaggcca tcgacaacct caaggctgcc gccaacatcg
    24121 ccaaggccgt cgcagcggat tggccgaagg agcccggggt cgacgaagac ctctacagtg
    24181 cgctcctcga actcggcgaa gcctatcgcg tctccggcga atacctctcg gccaagtcca
    24241 gctacgaagc cgcactcccg gtcatacgcc gggtggccga cgcggctcca aaggacgcct
    24301 atgcgctcaa cagcctcggc gttgtcaacg atcgcctcgg cgacatgatg atgttttccg
    24361 gcaatccaaa agcagcgatc gaattctacc ggaccgccta tgaactcatc cagatggcgc
    24421 agggtggcag caccaagagc acggtaatcg agttcaacag cgtcattatg ctgttcacac
    24481 ttgcgctcgc cgaaggattg tcgggcagcg acaagaacgc cgagattgcg aaactttata
    24541 cggaattccg caacctcctc gacctgaaga ccgagcagtc ggcgcaaaag cggtcgtatt
    24601 tggcgagcga acttttcagg ctcgtcgtca atccggtggt tgcccgccag cgcctcgtcg
    24661 aatccatggc ggtttatgat cgccaggaca aatcacggaa gctgcgcgac gacgagcgcc
    24721 tctggtatcg cgtcatcgac agtggattgc gcgcgttgcc gtgacggccc cagcagccgc
    24781 gcgatcggac ctcgcgtgtc ccgatcgcgc gccgctcctc gcccgagcgc ccgccgcccg
    24841 ggcgttcaat ggcgccatgc tgccgaaggt ggctccccac cagaaggtgg cggccatggc
    24901 cgcaagccag ccgattgtta tctggctttt cgcggcacct tccgacatac gcccctccgc
    24961 tccccggaga cggacgatcg gtctacgggc gcccgcggcc atgcaaaatt gccagaggcg
    25021 gtccgacaag cggcccgtca caggtctgaa acagaggaga agacctgtcg gctcacgctt
    25081 tcggcgcgtc gaccgtcacc acttcgccct tgcgaacgac gatcgccacc tcgccgcgtt
    25141 tgaggcgcaa ggcgtcggcg ccgaagagct cgcgccgcca gcccttgagc accgcgatat
    25201 cgggctcgtc ctcggtcgcg agccgctcga gctcgtcggc atcggctatg agccgcggcg
    25261 cgacgctgtg ccgggcggct gccgccttca ggagcacgcg gaggagatcg accagcgcat
    25321 tggcatccgc agagagtgcc gggccgccgg gaatgggcgg gaccgtcttc aggtcgcggg
    25381 cgaggccacg cttcaccacc tcgatgatct ccttggcgcg ctgcgagcgg gcgaacccgt
    25441 ccgacagcgt ccgcagcccc gagagggcgg caacgtccgt cggcgcctgg ttggcgacgt
    25501 catagagcgc ctcgtcgcgc agcacccggt tgcgcggcac gttcagcgat tgggcgaggc
    25561 cgtcgcgcca ggcagcaagc tcgatcagga cggcgaggct ccgccggttg cgcactttga
    25621 gcttcagccg cttccaggcg tcctcgggcc gcagcacata ggtctcagga tcgccgagcg
    25681 ccgccatctc ctcggcgagc caggcgtggc ggccggtcga cttgagtttc tcgtcgagct
    25741 ggacgtagac atcgcgcagg tgcgtgacgt cgcccaacgc atagacgagt tgcttctgcg
    25801 agagcggccg gcgcgaccaa tccgtgaagc gggacgactt gtcgagatcc tggccgagca
    25861 ctcgcttcac gaggttcaca tacgaaatcg attcgccgaa gccgcacacc atggccgcga
    25921 cctgcgtgtc gaacagcggc gccggcaccc gtcccgacat aatgtggacg atctcgatgt
    25981 cctgccgcgc ggcgtggaaa accttcaccg gcggacgatc ggtcatcagg ctgacgagcg
    26041 gcgcgaggtc gatgccgccc gccagcggat cgatcaggaa ttcctgattg ggcgatggtc
    26101 cggccagctg cacgaggcaa agctgcggcc agaacgtctg ctctcgcatg aactcggtat
    26161 cgacagcgac gaagcgctca ttggcgaggc ggtcgcagat ggcagcgagc tctgtggtcg
    26221 tctcaatcaa tctcatgacg atcgtatagt cgaacgccgc gcgaatgtcc cgcatggcag
    26281 ctgcgcgaag tcggcgcagc tgacacagac gtctcagact tcagtcgaat acgataatct
    26341 ggcgcaccac ctgggcgtcc gccagcctgt cgaagccttc attgacctcg tcgagccgga
    26401 tgaaggcgct cctcaaccga tcgacgggca gcttctggcg cttgtagagc tcgatgtagc
    26461 ggggcatgtc gcgcgcgatg acgcagccgc ccatgtagct gcctttgacg acacgcgcat
    26521 cctgcaccat gccatagtgc tggagcgaga atagccccgt gaccggcggc aggcccgcgc
    26581 tgatcgtctg accgccgcgc acggtgatct tgtaggcgag ctccatggcc ttcaccgagc
    26641 ccgccatctc gaatgccgtg tcgacgccgc catccgtcgc cttcacgatt gcctcgacga
    26701 cgccggggtc gcgcgcgttg aatgtctcgg tcgcaccgag ctggcgggca agcgccagtt
    26761 tgtcgtccga gaggtcgacc gcgatgatgc gctcggcccc cgcaacgatg gcgccgagca
    26821 ggcaggagag gccgacgccg ccgagaccga ccacggcgac gacgtcgccg ggcttcgttc
    26881 cggccgtatt caccgcggcg ccgacgcccg tcaccaccgc gcagccgaac atggcggcat
    26941 cgtgcatcgg cacggccttg tcgatcttct gcacggagtt gcgcggcacg accgcgtgat
    27001 cggcaaagca cgagagcccc gcgtagtggg cgacgggcgt cccgttgagc gaaagccgcc
    27061 ggccgccggt cgcgaggtcg cccttctgct tggctgcatt gaaggtcgtg cagaggtttg
    27121 gccggccgga cacgcactgc cggcacgccc cgcagctcgg ggcgaagatg aagacgacgt
    27181 gatcgccgac cgcgagatcg ctgacaccgg caccgagcgc ggcgaccacg ccagcgccct
    27241 cgtgaccgat gagcgtcggc agcggccgcg tgcgcgcacc ctcgatcgtc gagagatccg
    27301 agtggcagag cccggccgcc ttcaccttga cgagcacctc gcccgcccgc ggcccctcga
    27361 gatcgagcgt ctcgatccgg agcggcttcg acgtggcgta aggccgtgac aggccctgct
    27421 catagaggac ggcgccttga attttcatgc gatcatctct ggatgtggag ccggagccgg
    27481 ccctcggtcg gtttggttca cccggaccga ccatgccaca ggttccgtcc cacgctccag
    27541 ggcgcggccg tcgcaccgga acacactatc ctggaacagg ctgtccttta cgccgccatt
    27601 gcccaggagt acgctcgctc gattgttcga gccgacaggg ctggctcggg gacttttcga
    27661 atcgtttgtt ttctcagggt ggggctatgc acagccttct cggggcgttg gccgttctca
    27721 gggcaccgat cgtggtggcc gtgattgctt acctcgtgct gctgcttcct ggccagtcgg
    27781 agcagatttt catcaccatt tcggaaatga cactctggac gaggccgggg cggctggttg
    27841 ccacggtgtt tcgagttctg ccagcggcaa tcctgctcgg tgccgtgttt ctcatttgcg
    27901 cgcgccgcct gatccgggcc gtgccggcct accggttggt cgcggccaaa cgccatgcgg
    27961 agctgctcga cgcgatctca ctcgtgctgg ctcccgttcc gatcaccctg ctgctcgtgg
    28021 cctacgttcg ggccgtgacg gagtggccga aaagtgtcgg gctgatcgtg cccgcggccg
    28081 ccttcggagc cgtcgcctac ctgacctcgc gaaaccgagc acgcaggcat ctcactctag
    28141 tcgcggaccg gatgtgcgcg atggtcggcg agacgagcgt cggacgcgtg ttgatgcgcg
    28201 tctcgatcgt gatcatgacg ctcaatgtgc tgatcgcgag ttggccggtt cagttcggga
    28261 gcctgctcgg cccgatcgcg gtcgttttcc tgtttctagc cgtactgtgc gtcgccgcct
    28321 cactgctgac ccacgtctat gaccgcttcc aggtgccggc gattccggca ttggccgtgc
    28381 tggcactggt cgctggcacg tacggcttaa atcacaatca cgagttgcga gtgctttcga
    28441 aggaaccccc gcctgcggca ccggcgcttg ccgacgcgtt caataaatgg cttgagaacc
    28501 ggccggacat cgatgacttc gaggggcggc cgtacccggt gtatctggta tccgcagaag
    28561 ggggtgggct ttatgctgcc atccatgcag cggcgacgct cgccgccatc caggacaact
    28621 gcccggcgtt cgcggcccat gtcttcgcga tcagtgcggt ctcgggcgga gcgctcggcg
    28681 cggcggtttt caactcgatc gtgcgtgcca agccgccgcc gcggaacctc cccaaggacg
    28741 ctcgttgtcc cccgcctcag ggagacaaca acgagaggca gaagctcgtc agggagtttt
    28801 tcgatcggga cttcctgaca ccggtcatgg ccgcgggcct cttccccgat ttcttccagc
    28861 acttccttcc ttggccgatc ggcgccttcg accggtcgcg cgcgctcgag gcgtcgtttg
    28921 aaaccgcctg gcgcgaggtg atgcgcgaca atccgaatcc ggttcgaaac tacttttcga
    28981 gtgacttccg ctcgcactgg gatgcgggtg cggatgcacc tgcgttgctg ctgaatacga
    29041 ctgtcgtcaa gtccggcggt aatatcctcg cagcgccgtt ccgtgtagtt agcaaatgga
    29101 gcgatgaggg gaagatggat gtgctcctca gaacaggcac ttggtcactt gcggcgtccg
    29161 ttggcctgag cgcgcgattc ccaatcgtca cgccacctgc gtgggtcaag ccgcatggga
    29221 ccgcgtcctt cgaggttatg gatggcggct acttcgacaa ctcaggcatg tggaccatca
    29281 tcgatcttgc gcagcagctg ctcgaagccg ccaaaacggg cactggcgac aagctggctg
    29341 cggggagtgg ccaggaggtc tgtgcaccag gaaatcttgg agtgcttgag gttcgcggca
    29401 ggcgccaacc cttctgcgtg aagctcctga agatcagtgc ttgggccgac cccagcgatc
    29461 gatccgttca aggcgagctc gcagcaccaa tgacagcctt cttcagtgct cgggttgccc
    29521 atactcacct ggctggtatc gtcgccaaac gccagtactg cggcgatgcg aaatgtgggc
    29581 tcggccaaaa atcgcttcag cctcacgtgt attttcgacg cctcaagctc agtagacgct
    29641 tgccgctcgg ttggtactac tctcgggaaa cggtcaaggg tctcatcgca agtacggcgc
    29701 cgcgagctgc gtgctcagat acggtcgcgc cagacagtca cgatgagcag ctcaaggaga
    29761 tcgagatgga gaacagctgt ctattttcgc gaattgccgc cgatctgcgt ctgtaaccca
    29821 acgataatgg agacacccat gctcgccatt cgctctgcgt tctcgttggc cttcctgatc
    29881 gcgggtagca tctcgatgct gacccaattg tcgaccgagg cgcgtgggca gtcgcgttgt
    29941 gagagccgat cgagcgcgtc ctgcacgaag aagggggaga gtttctcttg cagcggtata
    30001 acatacccca aagagttcta ctgccgatgc tacctgactg atgacggatg tgtcgaggac
    30061 aatctcccgt cgcacatatt caggctagat acggcaacgg atgccgtcaa ggagctgcag
    30121 aaaaaactcg gcgtcaaaaa ggtgaccg
//
LOCUS       DAORNH010000016        30124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1172_length_30124_cov_2.752370, whole genome shotgun sequence.
ACCESSION   DAORNH010000016 DAORNH010000000
VERSION     DAORNH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 30124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30124
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_30124_cov_2.752370"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK264_03110"
     CDS             <1..768
                     /locus_tag="PK264_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74917.1"
                     /translation="VGAPKALAPTAEALPSPVRSAITTGVPDTIAEPERYAALTSLDE
                     LSSHIAQRATGAHGAARTMVVGSGHDNSTVGDARDLALTLADAGRKVIIVEWSTGASR
                     LVEDIGADDGAGMSELLSGAATFEDVIRRLPGSEAHFLTGGGTLAVLGGEEAERVNLV
                     LDALDEAYDHILITGDRPNVKRLFEIIQGRIDTGVLIDAGGVSRPNDAAPGTFLGYDV
                     TDIDVVRLDPNGVHQSRRAPKAARNGSVAGPQTPRVV"
     gene            982..2040
                     /locus_tag="PK264_03115"
     CDS             982..2040
                     /locus_tag="PK264_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRD74918.1"
                     /translation="MSRRSTSLFKAALSALHYSGTDSMLAPLTRGAGVIFMLHHVRPE
                     KPDAFEPNRILKITPDFLEQVIKHVVEQGFEVVSLDELHYRMTEGDHERPFACFTFDD
                     GYKDNREHAYPIFKRYGLPFAIYVPSDYADGRGDLWWLALERIISSVDALSLKMDGTM
                     RKFKCATPADKDTAFHTIYWWLRGINEDYARQVVRELGNFINLVPEELCASQVMSWDE
                     IRAIAADPLVTIGAHTRRHFALARLSISDARAEVAESVKRIERELARPCRHFSFPYGD
                     ASSAGGREFELARELGLKTAVTTRKGLIHPHHAKSLTALPRLSLNGDFQKLRYLKVML
                     NGAPFSLWSKVERLAKAG"
     gene            2123..2839
                     /locus_tag="PK264_03120"
     CDS             2123..2839
                     /locus_tag="PK264_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRD74919.1"
                     /translation="MPLKPATRDKLKTVSTATLTTALFKRGFRVQMIQNVQPLDPGKP
                     TMVGEAFTLRYIPAREDLNPITVFSDRGHPQRKGIEDCPPGAVFVIDSRKDARAASAG
                     SILVTRLMKRGVAGIVTDGGFRDAAEIAKLDIPSFHQRPAAPTNLTLHQAIDTNVPIA
                     CGDAPVFPGDVIVGDADGCVVVPAHLADEIADEAVEMTAYEDFVTEKVLEGRSIMGLY
                     PPTDEQSKVDFAAWRKVKGR"
     gene            2946..3803
                     /locus_tag="PK264_03125"
     CDS             2946..3803
                     /locus_tag="PK264_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74920.1"
                     /translation="MSAGKFRFRIALSVGALIAFILTFPFMLALLVDASGCRRGGGGA
                     CGAVAVVIGMFGKPLGVLILGLVMICFMLSRARAIGLGFGWAAATLAWFLAALPLLVM
                     VGNFWGANFGLGLVYATFPTLILFLVVWIAFLSIADVATATNDDADVGIAWMVAIGAA
                     VHGLIVGAMTVWSGLITIPYLGASLGMGVMKMMMDLVKVSNIAALGMPMNVMLLIDVV
                     VFAAALGFIVLKGSGGSEADHEAATAPIKAAPSRGAARPQPSPARASAPKSGQPRAAF
                     GTRAVRPKS"
     assembly_gap    3983..3992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3993..4255)
                     /locus_tag="PK264_03130"
     CDS             complement(<3993..4255)
                     /locus_tag="PK264_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD74921.1"
                     /translation="MAEQMQRIPVHTDIKPENPGHLASRYLDVPNMPWEKTKFPGIEI
                     KVLYSDDSGATTALFKLAPGAIVPLHEHTALEQTYVLEGSIVDA"
     gene            complement(4355..5605)
                     /locus_tag="PK264_03135"
     CDS             complement(4355..5605)
                     /locus_tag="PK264_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD74922.1"
                     /translation="MTADRHSTEVADVAIAGATFAGVALALALADVLPGIAITLIDQR
                     AHESDAPERDPRAFAIAAGSRNLLDAIGVWTRVATEAEPVRAIDITDGSLDAAVRPVI
                     LGYDNALSDGEPASCIVPAEALRHALFDALAAKGAGIQHVRGAKAVAVIAGAGAADVR
                     LADGSLVKARLLVDADGRNEALRDAANIGSVGWSYDQIGIVTVVTPERPHEGRAVQHF
                     LPAGPFAILPMTGNRCCITWSEEAVRGRAILALDDQAFLAEVEQRFGPRLGPLTLAGP
                     RASWPLSLRLSRELVGTRLALVGEAVRLVHPIAGQGLNLALRDVAALAEVLADGIGLG
                     LDPGDGEILRRYARWRRFDGWASSAAYDGLNRIFSGDNPLARTLRTAGLGLVDRLPEL
                     KRWLVAEAAGQTGDVPRLLRSSRI"
     gene            complement(5622..7358)
                     /locus_tag="PK264_03140"
     CDS             complement(5622..7358)
                     /locus_tag="PK264_03140"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRD74923.1"
                     /translation="MINTLKIALAQLDPTVGDIAGNVERVKRARTEAIRLGADLVLFP
                     ELFITGYPPEDLVLKPAFAAASRRAIESLAPLTAEGGPALLVGGVWPDDGHIYNAVAL
                     VDRGAVQAVRYKVDLPNYGVFDEKRVFTPGPLPGPVQFRGVRIGVPICEDIWKDEVVE
                     CLSECGAELLLSPNGSPFDWPKPDMRLNVAVARVTESGLPIAYLNQVGGQDELVFDGA
                     SFVLNGDKRLAVQLPAWEEAVALTTWERTPQGWRCSVGPVAKIEENDAAAYHACMLGL
                     RDYVTKNGFPGVVLGLSGGVDSALVAALAVDALGPERVHCVMLPYRYTSGDSLTDAAA
                     CAKALGVRYDTIQIAPAVEGLTAMLGPLFAGRKPDITEENLQSRVRGTTLMAISNKFG
                     AMVLTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYRLCRWRNANVAKGGKGPSG
                     IVIPERIITKAPTAELRENQKDQDSLPEYEVLDDILACLVEREMPLAEIIARGHPAET
                     VKKVERLLYLAEYKRRQAAPGVKISQRNFGRDRRYPITNRFREDVAAMPASPGPAAPR
                     MVKPRADGGDVS"
     gene            7535..>9113
                     /locus_tag="PK264_03145"
     CDS             7535..>9113
                     /locus_tag="PK264_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRD74924.1"
                     /translation="MLDGKTIISLALGYVGLLFVIAWLGDRFMRRGDGNGRPIIYALS
                     LAVYCTSWTFFGSVGLAARTGYDFLPIYIGPILVFLFGWRLIQRVVRLAKSQNITSVA
                     DFLAARYGKSAEVAAIVALIALVGTLPYIALQLKAVAVSVETLLGRSPFGDLMPAEGM
                     PIDTAFIVMMALAIFAVLFGTRHIDATEHQHGLMLAIAAESLVKLATFVAVGAFVTFG
                     IFGSVDGLAIRASQSLELSGVFGKGINGSNWLVMLVLSSVCILLLPRQFHVTVVENNS
                     ETEIRRARWLFPLYLIVINIFVIPIAAAGLILLPANIVDQDMYVLALPMWANADVMTM
                     LAFVGGLSAATAMVIVECVALAIMLCNGVIVPLLLRHRVVAEQPVEDVSQLLLLIRRC
                     VILVILVLGYAVYRALAQGQGLASIGLVAFAAIAQLAPAFFIGLIWRRATARGAIAGI
                     LAGFAVWSYTLLLPWVVKSGWLPASIIEHGPLGIGVLRPQALFYLQFEPLTHGVLWSL
                     ATNVVCYVVFSLLKTPEP"
     assembly_gap    9114..9123
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9124..>10802
                     /locus_tag="PK264_03150"
     CDS             <9124..>10802
                     /locus_tag="PK264_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HRD74925.1"
                     /translation="QASLFVAEDMPRAAPSASFRIWRTSVTVGDLQQLAGRYLGTDRA
                     ARSFSEYAIGRGQPLVSHAEADIGSIRFTEHLLTSAIGAASSRLVLTLLLRRGNVGRQ
                     SDLRLLDDASEALQYNRDLLQSALDQVRHGLSVFDKDLRLICWNRQFRELLELPQDLG
                     RVGVPLDRILRVIAARGDAGGGSIDAHVSTRLMQLAVKHETFQERIGACGRILEIRTS
                     PMPQGGIVTTYSDITERVQAANALAEANDALEGRVVERTAELTKVNGELIVAKAAADA
                     ANLDKTRFLAAASHDILQPLNAARLYATSLSESLGRGAPIDAPTIARNIDASLGAVEE
                     ILSTLLDISRLDSGNLQPEISAFPLQPLFDQLTVEFQPLAAERRLALRFKPTSHWVES
                     DRRLLRRVLQNLIANAIKYTPTGTVLVGCRRRGSTLSLEVLDTGPGIPEAKRELIFKE
                     FQRLEETATSVKGLGLGLAIVERICRVLGTPITVASVLGRGSRFAVSVRKAAPALRPR
                     PSDASMDRMTGLGVNVGGLLVLVIDNEPDVVSGMRLLLEGWGCDVITANSI"
     assembly_gap    10803..10819
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10820..11078
                     /locus_tag="PK264_03155"
     CDS             <10820..11078
                     /locus_tag="PK264_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74926.1"
                     /translation="GHQRRLPDVILADYHLDHGTGLDAIAVVCATTQTSIPAVIITAD
                     RSPGLQRDLKLAGHAYLSKPVKAGALRALLAQLALRRNAAE"
     gene            11247..12230
                     /locus_tag="PK264_03160"
     CDS             11247..12230
                     /locus_tag="PK264_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD74927.1"
                     /translation="MITRRTILKGLAASATGTVALGGYAFAIEPMRGPVISGYHLTPP
                     RWPVGLKLRIAVLADLHACEPWMGPRRIEHICDTAMRLKPDLIVLLGDFTTAHRYISG
                     QVPAKTWARILGGLKAPLGTWSVLGNHDWWADHDAQWRGDGPVEARIELERNGIPVLE
                     NDAIRLTKDGQAFWLAGLGDQIAFVLSRLSPGSRRARALASVDEAALRSGPASFTSRG
                     RRFAGIDDLPGTLAKVTDDAPVILLAHEPDIFPQVPDRVALTLSGHTHGGQVRVLGWS
                     PVVPSRYRNRFAYGHLSEGERSIIVSGGLGCSIMPVRFGVPPEIVTVDMGA"
     gene            12232..13293
                     /locus_tag="PK264_03165"
     CDS             12232..13293
                     /locus_tag="PK264_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRD74928.1"
                     /translation="MARTAGEARGAQALMAGAGLALACVFAALVLLLTAAETVAQPAA
                     TASSKAAETQRLRLLAGVKSWGYQLRQLKFDELAASPFDLLVIDHALSVMRRFQHEFD
                     RERLGPVKIKPDGSRRLLLAYLSIGEAERYRFYWREDWEKSPPAWLAEENKEWPGNYN
                     VRFWDPEWQRIIFGTPESYLERMIRAGFDGVYIDRADVSLEWQKSRPTAEAEMVKLLT
                     SLAAHARRIDPHFLIIMQNAEELLASAEVMSAIDGLAKEDLFYGIDHKASPNDKETVD
                     WTIEALAPLRRSGRRILLVEYLDDPAKIADVIARARLHDYVVHFTVRDLGVLSTTPPS
                     APRAAAPGAPALPPPTRVP"
     gene            13370..13933
                     /locus_tag="PK264_03170"
     CDS             13370..13933
                     /locus_tag="PK264_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HRD74929.1"
                     /translation="MAEDLSFRQDELPADKSLIIVDDDRAFLQRLVRAMEQRGFEVRF
                     GHSVAEGLDLIRQKAPAFAVVDMRLEDGNGLDVIAELARVRADSRTVVLTGYGNIATA
                     VSAVKLGAVDYLAKPADADEITDALLAPTHEKAPPPENPMSADRVRWEHIQRVYELCN
                     RNVSETARRLNMHRRTLQRILAKRAPK"
     gene            14004..14870
                     /locus_tag="PK264_03175"
     CDS             14004..14870
                     /locus_tag="PK264_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HRD74930.1"
                     /translation="MSNRYVYVDGRFLAWHEASVHAEDRGFQFGDAVYEVIEIRDGRL
                     VDERRHMDRLERSLAELRIDAPMSRAAWAPVLREAIRRNRVRDGSIYLQVSRGCSPRD
                     FLFPPAGTPPTVVVIARHGSRAKVEATAAQGIGIISMPDNRWGRCDIKTVMLLPASIA
                     KEAARATGAKEAWFVDDKGFVTEGGSSNAWIVDKDGRVITRKCDEALLRGITRTTLID
                     LLKKDGIELVERPFTIEEAKAAREAFVTSATNIVMPVVSIDGRPVGNGAPGILTQRLR
                     ERFHSVAETSAA"
     gene            15001..15252
                     /gene="hfq"
                     /locus_tag="PK264_03180"
     CDS             15001..15252
                     /gene="hfq"
                     /locus_tag="PK264_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HRD74931.1"
                     /translation="MADRPMSLQDTFLNHVRKQKTPLTIFLINGVKLQGIVTWFDNFC
                     VLLRRDGHSQLVYKHAISTIMPGQPVHLMDADPADGESK"
     gene            15236..16702
                     /gene="hflX"
                     /locus_tag="PK264_03185"
     CDS             15236..16702
                     /gene="hflX"
                     /locus_tag="PK264_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRD74932.1"
                     /translation="MANRNKRSGDEIATDDDDATHPGGSADERPSEAFDHSHVTTRAI
                     VLVPVLRSASDRGRSRRRSGTAQAPGAPVAQHSPEDRLAEATGLAAAIDLEVASALIV
                     PLAGIRPSTLLGSGKVEEMAALVKSTGAGLVIVDHAVSPIQQRNLEKALETKVLDRTG
                     LILEIFGRRARTREGRLQVELAHLTYQKSRLVRSWTHLERQRGGFGFIGGPGESQLEL
                     DRRLIQDRIDAIRRELAVVVRTRELHREGRRRVPYPIVAIVGYTNAGKSTLFNRVTGA
                     SVLAKDQVFATLDPTMREVKLASGRRIILSDTVGFISDLPTMLVAAFRATLEEVIEAD
                     LVLHVRDISHAETDAQRADVESVLAGLGIEAQSAAAPVFEVWNKIDRLTAEARSELGG
                     QAERSERSPALVSAVTGDGIAALLQAIDRRLSGSDEIVRLMIPPSEGGVLAWLHANTD
                     VTGREIAEDGTVTATVRIDTAKKGRLLGRISGTAARIA"
     gene            complement(16738..17376)
                     /locus_tag="PK264_03190"
     CDS             complement(16738..17376)
                     /locus_tag="PK264_03190"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRD74933.1"
                     /translation="MEFDFETMPQNDRYKILISTVVPRPIAWVTTLDDKGTPNAAPYS
                     FFNAMGTNPPIIVIGIQAHAEKRLKDTGHNILNSAEFVVNFVNEANAEAMNVTCIDAP
                     PGVDELKLAGLGTVPSVKVAPPRIAGSPVAFECRVHTSLTFSPNQAIVIGRVVQAHVD
                     DSFVIDPKRCYIDTPKMKLVGRMHGSGTYTRTNEQFVLDRPEWADWVKKGQV"
     gene            complement(17428..18276)
                     /gene="kdsA"
                     /locus_tag="PK264_03195"
     CDS             complement(17428..18276)
                     /gene="kdsA"
                     /locus_tag="PK264_03195"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRD74934.1"
                     /translation="MTKVETAGPAPEVKVGNVTFASRRPIAVFAGPCQMESRAHALEM
                     ASALAELSRRLGIGLVYKSSFDKANRTSLSGKRGIGLDGALAVFAEIKEKLGLPVVTD
                     VHEAAQCAVVAPVVDVLQIPAFLCRQTDLLVAAAKTGRVVKVKKGQFLAPWDMKNVVA
                     KITGSGNRNVLVTERGASFGYNDMRSLPIMAETGAPVIFDATHSVQQPGGQGTSSGGD
                     RRYVPVLARAAVAVGVAGLFIETHQDPDKAPSDGPNMVPLRDLGALLGELIAIDRVVK
                     ATVAPA"
     gene            complement(18290..18820)
                     /locus_tag="PK264_03200"
     CDS             complement(18290..18820)
                     /locus_tag="PK264_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRD74935.1"
                     /translation="MNHDRYDDAYIAAILNDCQSIAMVGASANTNRPSYFAMKYLLSK
                     GFRVIPVNPGLAGQKILGQPVAAALGEIEGKVDMVDIFRNSEAALEVTREAIALKEKL
                     GLKVIWMQLSVANEVAAVEAEAAGLRVVMNRCPKIEYGRLSGEIGWAGVNSGTISSVR
                     PKLGAGGVQNHVIGKR"
     gene            complement(18817..19260)
                     /locus_tag="PK264_03205"
     CDS             complement(18817..19260)
                     /locus_tag="PK264_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD74936.1"
                     /translation="MTDTRFEPRLSLVTLGVADIAASRQFYEALGFTASAASSNDNVA
                     FFQLGAMALALYGRQALAEDAGVVAGSGEEFSGVTLAHNARSEAEVDDLVAHAVASGA
                     TLVKQPCKVFWGGYSGYFADPDGHLWEVAHNPFFPLDDEGRLTLP"
     gene            complement(19264..20091)
                     /locus_tag="PK264_03210"
     CDS             complement(19264..20091)
                     /locus_tag="PK264_03210"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD74937.1"
                     /translation="MTTTATKSDASVDARPLKSETRGPVLILTLDRPAARNSLSEAMI
                     AALHAEIDRASAARDVRAIVLTGAGPAFCAGHDMKEMTARRSDSDGGRAYFRDLIGRC
                     SAMMQAIVRSPKPVIAAVQATATAAGCQLVASCDLAVANTDAKFATPGVNIGLFCSTP
                     MVALSRNVPRKRAMEMLLLGELMTAEQAADWGLVNRAVAADRVMPEALAMAELIASKS
                     SATVAIGKRAFYDQLELRLSDAYAYASEVMVENMLYRDAAEGIGAFIEKRKPDWKGC"
     assembly_gap    20688..20744
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20745..>22329)
                     /locus_tag="PK264_03215"
     CDS             complement(<20745..>22329)
                     /locus_tag="PK264_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRD74938.1"
                     /translation="EAETATDPTRIADIQIRLTDIEAHSAPSRAAQILAGLGFDEAAQ
                     RRACRELSGGWRMRVALAAVLFLKPEILLLDEPTNYLDLEGTLWLESYLKAYPHTVLM
                     VSHDRDLLNRAVGAILHLHRGRLELYSGGYDDFEEARREKQRLDLKLKKRQDDERRRI
                     EGFILRFKAKATKAAQAQSRVKALARMQPIAAQVEDRVVPFHFPGPAKVVASPLLRLE
                     GVSVGYGDRAVLANLDLRIDQDDRIALLGANGNGKSTFAKLIAGRLTAQRGEVIGAKR
                     IDVGYFAQHQLEDLVPGRRPYDYMVDLLPTASEAERRSRLGQLGFSSEKADTKCQSLS
                     GGEKARLLFGLTAFGGPHLLILDEPTNHLDVDSREALIQAVNEYPGAVILISHDRHLV
                     DACVDRLWIVRSGTVKPYDGDIDSYRAELLAERGVKTRQVAKGPLTSEPGRIEQRRAA
                     AERRLELAPLKKSMQAAEKRVEALTAEIAKIDASLSDSTLYTRDPGKAQKLVHERGLK
                     GRELAAAEEVWLAAIEAYES"
     assembly_gap    22330..22367
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22368..22630)
                     /locus_tag="PK264_03220"
     CDS             complement(<22368..22630)
                     /locus_tag="PK264_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD74939.1"
                     /translation="MLHINELSYRIEGKPIIEDATVAIPTGHKVGLVGRNGAGKSTLL
                     RLITGEIAPDLGSITIARGSRIGYIAQEAPGGDDTLIDWVLAA"
     gene            22921..>23840
                     /locus_tag="PK264_03225"
     CDS             22921..>23840
                     /locus_tag="PK264_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD74940.1"
                     /translation="MSTFLFLAVLIGLVFALAMKRAPMWLWAIAAAAFAYVTQAGLFN
                     GEFEPRWPGFLGVIAWLPVALLALLAVPPLRRAVLVAPAFRMIKGILPRVSETEQQAL
                     DAGTIGFDAELFSGRPDFGVLRAVPGIELSAEEKAFLDGPTEELCRMIDDWKVRHAEK
                     EIPEPIWDFVKRHGFLGMLISKSHGGLGFSAQAQSLILGKIASRSPDVVTIVMVPNSL
                     GPGELIEKYGTDAQKHHFLPRLAKGLEVPCFSLTGPTSGSDAATMRDIGWVTNGMHEG
                     RETVGIRLSWDKRYITLGPKATLVGLAFRL"
     assembly_gap    23841..23850
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23851..25426
                     /locus_tag="PK264_03230"
     CDS             <23851..25426
                     /locus_tag="PK264_03230"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD74941.1"
                     /translation="NLLGKGEDIGITVALVPANHPGVHIGRRHLPSGAAFPNGPNWGN
                     DVFIPMDWVIGGEKMVGQGWRMLMECLAAGRAISLPSSGAAGAKAMLRFSSAYGRVRK
                     QFGLPVAKMEGLEEPLARLVETAYVNEAGRAVTAAMVSRGEKPSVISAIMKYQSTERM
                     RRAVNDAMDLHGGRGICDGPSNYLQSAYQMVPVGITVEGANILTRSLITFAQGALRSH
                     PYLYKEVQACQDPDTSKGLKAFESAFLGHLSFSMSNAFGAFFHNVTGGIFGKVPANAG
                     DNAEWYRQLWRCSRSFALVADLTVALLGGGLKTKQKLTGRMADALSELYFLACILKRF
                     EDDGRPSADRHIVAYCAQNCLYRFQESMRGAIHNFPIALARMKMRALVFPLGARYKPA
                     PDRLGTKIVSLVLEPGEVRDRLTRYIYVSKDPKDPTGVLEVALEKVIAAEGAEKKLER
                     AIRAGTVRRFHGIDWIADAVAKGVLTEEEGRGLKEAEALTDRVIAVDHFDPVEVKPNY
                     MTAGHNAAAMTSRAAE"
     gene            complement(25487..25726)
                     /locus_tag="PK264_03235"
     CDS             complement(25487..25726)
                     /locus_tag="PK264_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74942.1"
                     /translation="MKSVTKPMLLAASGLALALGLLALPASETVARERLTVDEGLARC
                     TAWCGAHNRSGSKKFWQCYGRCEVYWKKNGSDAKH"
     gene            26049..28043
                     /locus_tag="PK264_03240"
     CDS             26049..28043
                     /locus_tag="PK264_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRD74943.1"
                     /translation="MADLAHLKDWRYAVDGEGIAWAVFDRAGESQNSLGRRPLEELIQ
                     IVEAVEAGAKDKSIRGLVFISGKEKGFIVGADVREFEQFRTAAEVEEAIKPVLAMLDR
                     IEALPVTVVAAIHGFCLGGGLELALACHYRIATRDDGTRVGFPEVKLGIFPGFNGTAR
                     SIRQAGPLAAMTSMLSGSMIRASAAKGMGLIDELVPTPLNLRWAARKAIDRKRRSEAK
                     GMLQSALRQWPLRGVLASRMRKEVAGKARPEHYPAPYRLIDLFDRHGGNLDAMKRAET
                     SAFAPLMVSDTARNLRRVFHLMEAMKAEAPRDLGWRPTRVHVIGAGVMGADIAGWCVA
                     SGLVATLQDLSEEQIKKGIEAQKKTLERKFRTRPEREAARARLIADPKGEGIERADVI
                     IEAIVEKLEVKQSLFRGLEGRLKPGAVLATNTSSIMIEDIAAGLANPARLIGIHFFNP
                     VAQLPLVEVIRGAGSAESELRKGCAFVTAIDKLPLIVKSCPGFLVNRVLAPYMLGAMQ
                     RLEQGESKEKLDEAARIFGMPMGPIELADSVGLDVCAHVGRILKLAPPGESTLDRLVA
                     AKKLGKKSGEGFYVWKDGKPAKGEVKAETAELGRLGHELVAPLVAECERCRDEGIVAS
                     ADMVDAGVIFGTGFAPFRGGPLTYAAARKLAPAETRVAAE"
     gene            28093..28431
                     /locus_tag="PK264_03245"
     CDS             28093..28431
                     /locus_tag="PK264_03245"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRD74944.1"
                     /translation="MTSAVETVSDGSTALTREHVLSLLREHEAAVHRYGVKSLHLYGS
                     AARDELTPRSDVDLFVDCDDVNAFGFVELLDLRGFLRDLLGRNVDLTSRAGLHRLLRA
                     KIEQSSPQVF"
     gene            28469..28798
                     /locus_tag="PK264_03250"
     CDS             28469..28798
                     /locus_tag="PK264_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRD74945.1"
                     /translation="MLDAIYTARELRSGMTVDVYVSAMARGQRRGVERWVEITSEASR
                     HLPRELTDAFPGIPWLRIRDVGNVIRHAHSSVDDQMIWRIATQHLDEPESVLQVMLAG
                     LAPDVDC"
     gene            28828..30117
                     /locus_tag="PK264_03255"
     CDS             28828..30117
                     /locus_tag="PK264_03255"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRD74946.1"
                     /translation="MTPTLRRVAIVGGVRIPFCRSNTLYADLSNLDMLTATLNGLVER
                     YKLAGVHIDEVVGGAVVTHSRDWNLAREAVLGTKLARSTPGITMIQACGTSLQAAMGI
                     AAKIAIGQIESGIACGSDTTSDAPIVLRKSFAQQLVKGQQAAKTLGGKIKLAMTAAKP
                     AAMVLETPSTAEPRTGLSMGEHCELMAKEWRIPRAEQDELAVASHQKAAEAYRTGWMD
                     DLLVPCAGVFKDNNLREDASVEKMAALRTAFDKTEKGTLTAGNSTPLTDGASSVLLAS
                     EEFAQARGLPILAYLVDAQTSANDFAAGEGLLMAPTIAVSRLLDRQGMRLQDFATYEI
                     HEAFAAQVLATLRAWEDPAYCKRYLGKDAPLGSIDRTRLNVKGSSLAFGHPFAATGAR
                     VMANLAKLLNVNGGRGLISVCTAGGMGVAAILEGARR"
BASE COUNT         4834 a        10046 c         9939 g         5163 t
ORIGIN      
        1 gtcggcgccc cgaaggcgtt agcgccgacc gcggaggcac tgccatcgcc cgttcgcagc
       61 gccatcacga ccggcgtccc ggacacgatt gccgagccgg agcgctacgc ggcgctcacg
      121 tcgctcgacg agctgtcgtc gcatatcgcg cagcgcgcga ccggcgcaca tggcgcggcc
      181 cgcaccatgg tcgtcggctc gggccacgac aactcgacgg tcggcgatgc gcgtgatctg
      241 gcgctcacgc tcgccgatgc cggccgcaag gtcatcatcg tcgaatggag cacgggggcg
      301 agccgcctcg ttgaggacat cggcgccgat gatggcgctg gcatgagcga gctcctgtcc
      361 ggggcggcga cattcgagga cgtgatccgc cgcctgccgg gcagcgaggc gcacttcctc
      421 accggcggcg gcactctcgc cgtgctcggc ggcgaggagg cggagcgcgt caatctcgtg
      481 ctcgatgcgc tcgacgaggc ttacgaccac atcctgatca cgggcgatcg tcccaacgtg
      541 aagcgcctct tcgaaatcat ccagggccgc atcgacacgg gcgtgctcat cgatgcaggc
      601 ggcgtgagcc ggccgaacga tgccgcgccc ggcacgttcc tcggctacga cgtcaccgac
      661 atcgatgtcg tgcggttgga cccgaacggc gtgcatcagt cgcgacgcgc accgaaggcc
      721 gcccgaaacg gctcggtggc agggccgcag acgccccgcg tcgtatgaga acctgagcgg
      781 gcattgaagt gagacgccat gcgggacgcc tccgcggcct ctgtcgcgtc cggcccaatg
      841 cttttcgaga tcacgaggcc ttaaccagca ggatccttcg gccgcccgtc gcgcggcgag
      901 gcggcattcc agcacgcatc ttgcatctgg ccgggcgaag gggctttagt accgcgtcca
      961 accagcacgt tcccggggcc catgagccgc agatcgacat cgctcttcaa ggccgccctc
     1021 tcggcgctgc attacagtgg caccgacagc atgctcgcgc cgttgacgcg gggagcgggc
     1081 gtgattttca tgttgcacca tgtgcgcccc gagaagccgg acgccttcga gccgaaccgc
     1141 attctgaaga tcacgcccga tttcctcgag caggtgatca agcacgtggt cgaacagggc
     1201 ttcgaggtcg tctcgcttga cgagcttcac taccggatga ccgagggcga ccacgagcgc
     1261 cccttcgcat gcttcacctt cgacgacggc tacaaggaca accgcgagca cgcctacccg
     1321 atcttcaagc gctacggtct gccgttcgcg atctatgtgc cctccgacta cgctgacggt
     1381 cgcggcgatc tttggtggct cgcgctcgag cgcatcatca gcagcgtgga cgcgctctca
     1441 ctcaagatgg atggcacgat gcgcaagttc aaatgcgcga caccggctga taaggacacc
     1501 gcattccaca cgatctactg gtggctgcgc ggcatcaacg aggactatgc gcgccaggtt
     1561 gtgcgcgagc tcggcaactt catcaacctg gtccccgagg aactctgcgc gagccaggta
     1621 atgtcgtggg acgagatcag ggcgattgct gccgatccgc tcgtcaccat cggggcacac
     1681 acgcggcgcc acttcgcgct cgcccgcctc tcgatctcgg atgctcgtgc cgaggtggcc
     1741 gaaagcgtga agcgcatcga gcgcgagctt gcccgcccgt gccgccattt cagcttcccc
     1801 tacggcgacg cctcgagcgc gggcggtcgc gagttcgagc ttgcgcgcga gcttggcctc
     1861 aagacggcgg tgacgacgcg gaaggggctc attcatccac accacgccaa gagcctgacg
     1921 gcgttgccgc ggctctcgct caacggcgat ttccagaagc tgcgctatct caaggtgatg
     1981 ctgaacggcg cgcccttcag cctctggagc aaggtcgagc ggcttgcgaa ggcaggctga
     2041 gcgcttcgca cgcgctcatt tgcggtgaca cgtcgcgccc tctgaagcta cgttggcatg
     2101 gcccacacgc tcggagaccg acatgccgct gaagcccgca acgcgcgaca agctcaagac
     2161 cgtctcgacg gcgacgctca cgacggcgct cttcaagcgc ggctttcgcg tgcagatgat
     2221 ccagaacgtg cagccgctcg atcccggcaa gccgacgatg gtcggcgaag cgtttacgct
     2281 gcgctacatc cccgcccgcg aggacctgaa cccgatcacg gtgttctcgg atcgcggcca
     2341 cccgcagcgc aaaggcatag aggactgccc gccgggcgcg gtgttcgtca tcgacagccg
     2401 caaggatgcg cgggccgctt ccgccggatc gatcctcgtc acgcgactga tgaagcgtgg
     2461 cgttgccggc atcgtcaccg atggcggctt tcgtgatgct gccgagatcg ccaaactcga
     2521 tatcccatcg tttcaccagc gcccggcagc accgacgaac ctcacgctgc atcaggcgat
     2581 cgatacgaac gtcccgattg cctgcgggga tgcgccggtg tttccggggg acgtgattgt
     2641 cggcgacgcg gatggctgcg ttgtcgttcc ggcgcacctc gccgacgaga ttgcggacga
     2701 ggccgtcgag atgaccgcct acgaggactt cgtcaccgaa aaggtgctgg agggccgctc
     2761 gataatgggg ctctatccgc cgacggatga gcaatcgaag gtggattttg ccgcctggcg
     2821 caaggtcaag gggcgatagc cgggcaacgc cgagcggacg ccacgcgaga aagtcagttt
     2881 atgcctaaac cgtccggcaa gcttgcccgg atatgatcgc gccctggtgc ggttccgggg
     2941 gtgtcatgtc ggcaggcaaa ttccgatttc gcatcgcact ttcggtcggt gcgctcattg
     3001 cgttcattct gacatttccg ttcatgctgg ctttacttgt cgatgcttcc ggatgccgtc
     3061 gcggcggcgg cggcgcctgc ggggcggttg ccgtcgtgat cggcatgttt gggaaaccct
     3121 tgggcgtact gatcctcggc ctcgtcatga tctgtttcat gctatcgcgg gcgcgcgcaa
     3181 tcggcctcgg attcggctgg gcggcggcca cattagcgtg gtttctcgcg gccttgccgc
     3241 tgctcgtcat ggttggcaat ttctggggtg cgaacttcgg gctcggcctc gtctacgcga
     3301 cgttcccaac tctcatcctg ttcctcgtcg tctggatcgc ctttctctcg atcgccgatg
     3361 tggcgacggc gacgaacgac gatgccgacg tcggcatcgc ctggatggtc gccatcggcg
     3421 cggcggtcca cgggctgatc gtcggcgcga tgacggtgtg gagcggcctg atcaccatcc
     3481 cctacttggg cgcgagcctc ggaatgggcg tgatgaagat gatgatggac ctggtcaagg
     3541 tctcgaacat cgccgcgctc ggcatgccga tgaacgtgat gctgctgatc gacgtggtgg
     3601 tgttcgccgc ggccctcgga ttcatcgtgc tcaaagggag cggtgggtcg gaggcagacc
     3661 acgaggcggc gacagcaccg atcaaggccg ccccgtcgcg cggggctgca cgtccgcagc
     3721 cgtcacccgc acgagcgagc gcgccaaagt cgggtcagcc gcgcgccgcg ttcggcacgc
     3781 gcgccgttcg ccccaagtcc tgacgagcag cggctccgat cgggccgcta ccgctgcccc
     3841 tcctcggtga agaacttctc gccgtaggcg aagcggttgg gcttcaggaa gaagccgaga
     3901 atgagcgctc ccttgggcgc gacggcctcg tgctggttgc cggccggtcg ccagacgaac
     3961 tggcccgggc cgcacacccc ttnnnnnnnn nngcatcgac gatgctgccc tcgagcacgt
     4021 aggtctgctc caaggccgtg tgctcgtgta gcggcacgat cgcgccgggc gcgagcttga
     4081 agagcgcggt cgtcgcgcca ctgtcgtcgg agtagagcac cttgatctcg atccccggga
     4141 acttggtctt ctcccagggc atgttcggca cgtcgagata gcgcgaggcg aggtggcccg
     4201 ggttctcggg cttgatgtcg gtgtggaccg gaatgcgctg catctgctcg gccatggcgg
     4261 gtctcctcgc gattgaactc gcgcaagctt aaggccattc ggtctcattg tgtaccagcg
     4321 ccaaggccac tcgcgatggc atcgggtggg ggcgtcagat ccggctgctc cgcaagagcc
     4381 ttggcacgtc tcccgtctgg cccgcggcct cggccaccag ccagcgcttg agctcgggca
     4441 accgatcgac caggccaagg ccggcggtgc gtagcgtgcg cgcgagcggg ttgtcgcccg
     4501 agaaaatgcg attgaggccg tcgtaggccg ccgaagaggc ccagccgtcg aagcggcgcc
     4561 agcgcgcgta gcggcgcagg atctcgccgt cgccagggtc gaggccgagg ccgatgccgt
     4621 cagccagcac ttcggcgagc gcggccacat cgcgaagtgc cagattgagc ccctgccccg
     4681 cgatcgggtg gacgaggcga acggcctccc cgacgagcgc caggcgcgtt ccgaccaatt
     4741 cgcgcgagag cctgagactg agcggccagg acgcacgggg accggcgagt gtcaacggcc
     4801 cgaggcgtgg tccgaagcgc tgctccacct cggcgaggaa tgcctgatcg tcgagggcga
     4861 ggatggcgcg cccgcgcacg gcttcctccg accaggtgat gcagcagcgg ttcccggtca
     4921 tcggcaggat cgcgaacggc ccggccggta ggaagtgctg gacggcgcgg ccctcgtgcg
     4981 ggcgctccgg cgtgacgacg gtgacgatgc cgatctgatc gtaggaccag ccgacggagc
     5041 cgatgttcgc agcatcgcgc agcgcctcgt tgcgaccgtc cgcatcgacg aggaggcgag
     5101 ccttgacgag gctgccgtcc gcgaggcgca cgtcggcggc acctgcgccg gcaatcacgg
     5161 caacggcctt cgctccgcgg acatgctgga ttcccgcgcc cttcgccgcg agagcatcga
     5221 atagcgcgtg gcgcagtgct tccgccggca cgatgcagga ggcgggctcg ccgtcggaga
     5281 gggcattgtc gtagcccagg atgacaggcc ggaccgcggc atcgaggctg ccatccgtga
     5341 tgtcaatggc gcggaccggc tcggcctcgg tcgcgacgcg ggtccatacg ccgatcgcgt
     5401 cgaggaggtt gcggctgccg gccgcgatcg cgaacgcgcg gggatcgcgc tcgggagcat
     5461 cggactcgtg tgctcgctga tcgatcaggg tgatcgcgat tccgggcagt acgtcggcca
     5521 gggccagggc gagcgccacg ccagcgaacg tcgcaccggc aatggcgacg tcggcgacct
     5581 ctgtcgaatg ccgatcagct gtcatggcgt caagttcgcc gtcacgacac atcgcccccg
     5641 tccgcgcgcg gcttcaccat gcgcggcgcg gccgggcccg gcgaggcggg catggcggca
     5701 acgtcctcgc ggaagcggtt ggtgatcgga tagcgacggt cgcggccgaa attgcgctgc
     5761 gagatcttca cgccgggtgc ggcttgccgg cgcttgtact cggcgaggta gagcagccgc
     5821 tcgaccttct tcacggtctc ggccgggtgt ccgcgcgcaa tgatctcggc gagcggcatc
     5881 tcgcgctcga cgaggcaggc gagaatgtcg tcgagcacct cgtactcagg gagagaatct
     5941 tggtccttct ggttctcgcg cagctccgcc gtcggtgcct tggtgatgat ccgctctggg
     6001 atgacgatgc ccgaaggtcc cttgccgccc ttcgcgacat tggcgttccg ccagcggcag
     6061 agccggtaga cctgcatctt gtacagatcc ttgatcggat tgaagccgcc gttcatgtcg
     6121 ccgtagagcg tcgcgtagcc gaccgacatc tcgctcttgt tgccggtggt cagcaccatc
     6181 gcgccgaact tgttggagat cgccatcagc gtggtgccgc ggacgcggct ctgcaggttc
     6241 tcctctgtga tgtcgggctt tcgcccggcg aacaacgggc cgagcatcgc tgtcagacct
     6301 tcgacggccg gtgcgatctg gatggtgtca tagcgtacgc cgagcgcttt ggcgcaggcg
     6361 gcggcgtcgg tgaggctgtc gccggaggtg tagcggtagg gcagcatcac gcaatggacc
     6421 cgctcggggc cgagcgcatc gacggcgagc gccgcgacca gggccgaatc gacgccgccc
     6481 gagaggccga gaaccacgcc cgggaagccg ttcttcgtca catagtcgcg aaggcccagc
     6541 atgcaggcgt ggtaggcggc ggcatcgttc tcctcgatct tcgccaccgg ccccacgctg
     6601 cagcgccagc cctgaggcgt ccgctcccaa gtggtgagcg cgacggcctc ctcccaggct
     6661 ggcaattgta ccgcgagccg cttgtcgccg ttgagcacga acgaggcgcc gtcgaagacg
     6721 agctcgtcct ggccaccgac ctggttgagg taggcgatcg gcaggccgct ctcggtgacg
     6781 cgcgcgacgg cgacgttcaa gcgcatgtcg ggcttcggcc agtcgaaggg cgagccgttg
     6841 ggcgagagca ggagctcggc gccgcattcc gagaggcact cgacgacctc gtccttccag
     6901 atgtcctcgc agatcggcac gccgatgcgc acgccgcgga actggacagg gcccggcagc
     6961 ggtccgggcg tgaacacgcg cttttcgtcg aagacgccgt agttggggag gtcgaccttg
     7021 tagcggaccg cctgcacggc gccgcgatcg acgagcgcga cggcgttgta gatatggccg
     7081 tcgtctggcc agacgccgcc gaccagcagc gccgggccgc cctctgccgt gagcggcgcg
     7141 aggctctcga tcgcccggcg cgaggcagca gcaaatgcgg gcttcaggac gaggtcctcg
     7201 ggcggatagc ccgtgatgaa gagttcgggg aataggacga gatcggcgcc gaggcggatg
     7261 gcctctgtcc gcgcccgctt gacgcgctcg acattgccgg cgatgtcgcc gacggtcgga
     7321 tcgagttgcg ccagggcgat cttgagcgtg ttgatcatgc gccgacagta gtcgcggagg
     7381 gttggcggtc gcaagcggcc gatgcatgcg ggcggcgaca atcgctgcca ctcgccgtat
     7441 gattgaccat cgcccaaggt cggccttcct gccgtcaccc cggcagcgga tgatcggctt
     7501 cggcttcgag accgcgcgct tcgacacgga tactatgctc gacggcaaga ccatcatcag
     7561 ccttgcgctc ggctacgtcg gcctgctgtt cgtcatcgct tggctcggcg accggttcat
     7621 gcgccgtggc gacggcaacg gccggccgat catctatgcg ctgtcgcttg ccgtttactg
     7681 tacgtcctgg acgttcttcg gcagcgtcgg cctcgctgcc cggacgggtt acgacttcct
     7741 gccgatctac atcggcccga tcctcgtgtt cctgttcggc tggcggctca tccagcgcgt
     7801 cgtgcgcctc gccaagagcc agaacatcac ctcggtcgcg gacttcctcg cggcgcgtta
     7861 cggcaagagt gcggaggttg cggccatcgt cgcgctgatc gcgctcgtcg gtacgctgcc
     7921 ctacatcgcg ctgcagttga aggcggttgc ggtctcggtc gagacgctgc tcggtcgcag
     7981 cccattcggc gacctgatgc cggccgaggg catgccgatc gacacggcat tcatcgtgat
     8041 gatggcgctc gcgatcttcg cggttctgtt cggcacccgc catatcgacg cgacggagca
     8101 ccagcacgga ttgatgctcg ccatcgccgc cgagagcctc gtcaagctcg cgaccttcgt
     8161 cgccgtcggc gccttcgtca cgttcggtat cttcggcagc gtcgacggcc tcgccatccg
     8221 ggcgagccag agcctcgagc tcagcggagt gttcggcaag ggcatcaacg gcagcaactg
     8281 gctcgtcatg ctggtgctga gttcggtgtg cattctcctg ctgccgcgtc aattccacgt
     8341 caccgtcgtc gagaacaatt ccgagaccga aatccgccgc gcgcgctggc tgttcccgct
     8401 ctatctgatc gtcatcaaca tcttcgtcat cccgattgcg gcggccgggc ttatcttgct
     8461 gccggcgaac atcgtcgacc aggacatgta cgtgctggcg ctccccatgt gggcgaacgc
     8521 cgacgtcatg accatgctcg cgttcgtcgg cggcctctcg gctgcgacgg ccatggtcat
     8581 cgtcgagtgc gtggcgctcg ccatcatgct ttgcaatggc gtgatcgtgc cgctcctcct
     8641 ccgccatcgc gtcgtagccg agcagccggt cgaggatgtc tcgcagctgc tgctgctgat
     8701 ccggcgctgc gtgatcctcg tcattctggt gctcggctat gcggtgtatc gtgccctggc
     8761 acaggggcag ggtctggcct cgatcggcct cgtcgcgttc gcggcgatag cccagctcgc
     8821 acctgccttc ttcatcggcc tcatctggcg gcgggcgacg gcgcgtggtg ccatcgccgg
     8881 catccttgcg ggatttgctg tctggtccta cacgctcctg ctgccatggg tggtgaagtc
     8941 gggttggctg ccggccagca tcatcgaaca tggcccgctc ggcatcggcg tcctgcggcc
     9001 ccaggccctg ttctacctgc agttcgagcc gctgacgcac ggcgtgctgt ggagcctggc
     9061 gacaaatgtc gtctgctacg tggtgttctc gctcctgaag acgccagagc cgannnnnnn
     9121 nnngcaggcc tcgctgttcg tcgccgagga catgccgcgc gccgcaccat cggcgagctt
     9181 ccgcatctgg cggacgagcg tgacggtcgg cgacctccag cagctcgccg gccgctatct
     9241 cggcaccgat cgtgccgcgc gatccttctc ggaatacgcg atcggccgcg gccagccgct
     9301 cgtcagccac gccgaggccg acatcggctc gatccgcttc accgagcatc tgctgacgag
     9361 cgcgatcggc gccgcctcct cacgtctcgt cctcacgctc ctgctcaggc gcggcaatgt
     9421 cggccgacag tctgacctcc gcctcctcga cgacgcctcc gaggcgctgc agtacaaccg
     9481 cgatctcctc caatcggcgc tcgaccaggt gcggcatggc ctcagcgtgt tcgacaagga
     9541 cctccggctc atctgctgga accgccagtt ccgcgagctc ctcgagctgc cgcaggatct
     9601 cggccgcgtc ggcgtgccgc tcgaccgcat actccgcgtg atcgccgcgc gcggcgatgc
     9661 cggcggcggc tcgatcgacg cgcacgtctc gacgcggctc atgcagctcg ccgtgaagca
     9721 cgagaccttc caggagcgta tcggcgcttg tgggcgcatc ctggagatca ggaccagtcc
     9781 gatgccgcag ggcggcatcg tgaccaccta ttcagacatc accgagcgag tccaggccgc
     9841 caacgcgctc gccgaggcca acgacgccct agagggccgc gtcgtcgagc ggaccgccga
     9901 gctcaccaag gtcaacggcg agctcatcgt cgccaaggcc gcggcggatg ccgcaaacct
     9961 cgacaagacc cgcttcctgg ccgcggccag ccacgacatt ctccagccgc tcaacgcggc
    10021 gcggctctat gcgacgtcgt tgagcgagtc actcggccga ggcgcgccga tcgacgcgcc
    10081 gaccatcgcc cgcaacatcg acgcctcgct cggcgcggtc gaggagatcc tctcgacgct
    10141 gctcgacatc tcgcggctcg attccggtaa tctgcagcct gagatctcgg cgtttccttt
    10201 gcagccgctc ttcgatcagc tgaccgtcga attccagccg ctcgccgccg agcgccgcct
    10261 cgcattgcgc ttcaagccga cgtcgcactg ggtcgagagc gaccggcggc tgctccgtcg
    10321 cgtcctccag aacctcatcg ccaatgccat caagtatacc ccgaccggca ccgtgctcgt
    10381 cggctgccgc cgacgtggta gcacgctgtc gctcgaggtt ctcgacacgg ggcccggcat
    10441 ccccgaagcg aagcgcgagc tgatcttcaa ggagtttcag cgcctcgagg agaccgcgac
    10501 aagtgtgaag ggcctcggcc tcggcctcgc catcgtcgag cgcatctgcc gcgtgctcgg
    10561 cacgccgatc acggtcgctt ccgtgctggg gcgcggctcg cgcttcgcgg tctcggtccg
    10621 caaggcggcg cccgcgctcc gaccacgccc ctccgacgcg agcatggatc gcatgaccgg
    10681 gcttggcgtc aacgtaggtg gcctcctcgt cctcgtcatc gacaacgagc cggacgtcgt
    10741 ctccggcatg cgcctgctgc tcgagggctg gggttgcgac gtcatcacgg caaacagcat
    10801 cgnnnnnnnn nnnnnnnnnc ggccatcagc ggcggctgcc cgacgtgatc ctcgccgact
    10861 accatctcga tcacgggacc gggctcgatg ccattgccgt ggtttgcgcg acgacgcaga
    10921 cgagcattcc cgccgtcatc atcacggccg accgctcgcc gggcctgcag cgcgacctga
    10981 agctcgcggg ccacgcctat ctctcgaagc ccgtcaaggc gggcgcgctc cgcgccctgc
    11041 tcgcccagct cgcgctacgc cggaacgccg ccgagtagcg cgggcggccg tcggaattcc
    11101 gtcgattcac ggatgattaa ccattggcgg cccaccacgg tctcgacaca aggggcgggc
    11161 atgatcgttg catactcacc ttgaagacct gctcgactac cgtcgttgcg aatcgccagt
    11221 cccgatactc gaaagcacat gaactcgtga tcacgcgccg caccattctc aagggcctcg
    11281 ccgccagcgc caccggcact gttgcgctcg gcggctacgc ctttgccatc gagccgatgc
    11341 gcggccccgt gatctcgggc tatcacctga cgccgccgcg ctggccggtc gggctcaagc
    11401 tccgcatcgc cgtactcgcc gatctccacg cctgcgagcc atggatgggc ccgcgccgca
    11461 tcgagcacat ctgcgatacg gcgatgcggc tcaaacccga tctcatcgtg ctgctcggcg
    11521 atttcacgac ggcacaccgc tatatctccg gtcaggtccc ggccaagact tgggcgcgta
    11581 tcctgggcgg gctgaaggcg ccgctcggca cttggtccgt gctcggcaat catgactggt
    11641 gggccgatca cgacgcgcag tggcgaggtg acggacctgt cgaggcgcgc atcgagctcg
    11701 agcgcaacgg cattcccgtg ctcgagaacg acgccatacg cctcacgaag gacggccagg
    11761 ctttctggct cgcggggctt ggcgatcaaa ttgccttcgt actctcgcgc ctctcgccag
    11821 ggagccggcg cgccagggcg ctcgcttcgg tggacgaggc ggcactgcgc tccggacctg
    11881 cctccttcac cagccgcggc cgccgctttg ccggcatcga cgatctccct ggcaccctcg
    11941 ccaaggtgac ggacgacgcc cccgtcatcc tcctcgcgca cgagcccgac atctttccgc
    12001 aggtgcccga tcgcgtcgcg ctcacgcttt ccggccacac gcacggaggt caggtgcgcg
    12061 tgctcggctg gtcgccggtc gtgccatccc gataccgcaa tcgctttgcc tatggtcatt
    12121 tgagcgaggg tgagcgtagc atcatcgtct cgggcggcct cggctgctcg atcatgccgg
    12181 taaggttcgg cgtacctccg gagatcgtca ccgtcgacat gggtgcgtga gatggctcgg
    12241 acggcaggcg aggcgcgggg tgcccaggca ttgatggccg gtgccggtct ggcgctggcc
    12301 tgcgtcttcg ccgcgctcgt cttgctgctg acggccgcgg agacagttgc gcagcccgcg
    12361 gccaccgcga gttcaaaggc ggccgagacg cagcggttgc gccttctcgc cggcgtcaag
    12421 agctggggct atcagctccg ccagctcaag ttcgacgagc tcgcagcctc gccgttcgat
    12481 ctcctcgtga tcgatcacgc gctttcggtg atgcggcgct tccagcacga gttcgatcgc
    12541 gagcggctcg ggccggtcaa gatcaagccg gacggcagcc gccggctgct gctcgcctac
    12601 ctcagcatcg gcgaggcgga gcgctatcgc ttttattggc gcgaggactg ggagaagtcg
    12661 ccgcccgcct ggctcgccga ggagaacaag gagtggccgg gcaactacaa cgtgcgcttc
    12721 tgggatcccg agtggcagcg cattattttc ggcacgcccg agagctacct cgagcgcatg
    12781 atccgcgccg gtttcgacgg cgtctatatc gaccgcgccg acgtcagcct cgaatggcag
    12841 aaatcccgac cgaccgctga ggccgagatg gtgaaactcc tcacctcgct cgccgctcac
    12901 gcgcgcagga tcgacccgca cttcctgatc atcatgcaga atgccgagga gctgctggcc
    12961 tcggccgagg tcatgtcggc aatcgacggc cttgccaagg aggacctctt ctacggcatc
    13021 gatcacaagg cgtcgccgaa cgacaaggag acggtcgact ggaccatcga ggccctggcc
    13081 cccttgcgtc gctccggacg ccgcatcctc cttgtcgaat acctcgatga cccggccaag
    13141 attgccgacg tgatcgcgcg cgcccggctc cacgattacg tcgtgcattt cacggtccga
    13201 gatctcggcg ttctttcgac gacgccgccg tctgcgccac gcgcggccgc gcccggggcg
    13261 ccggccctgc ctccaccgac gagagtcccc tgactttcga catgctgcaa cgcagcgctt
    13321 tgcctcggcg caacctcgag tataatgcgc agagtcaagg agacataacg tggcagaaga
    13381 tctttcgttc cgtcaggacg agctcccggc cgacaagtcg ctgattatcg tcgacgacga
    13441 ccgcgcgttc ctgcaacggc tcgttcgcgc catggagcag cgcggtttcg aagtgcgctt
    13501 cgggcactcc gtcgcggagg ggctggacct catccgccag aaggcccccg cgttcgccgt
    13561 cgtcgacatg cggctcgagg acggcaacgg cctcgacgtg atcgccgaac tcgcgcgggt
    13621 gcgcgcggac tcgcgcaccg tcgtgctgac aggctacgga aatatcgcga cagccgtctc
    13681 cgccgtcaag ctcggtgccg tcgactacct cgccaagccg gccgatgccg acgagatcac
    13741 ggacgccttg ctggcgccaa cgcacgaaaa ggcgccgccg ccggaaaacc cgatgtccgc
    13801 cgatcgggtc cgctgggagc acatccagcg ggtctatgag ctctgcaatc gcaacgtctc
    13861 cgagaccgcc cgtcggctca acatgcatcg ccggacgctg cagcgcatcc ttgccaagcg
    13921 cgcgcccaag tagtctcgcg cactttgcga ccctggcccg cccaccgcaa ctcatccaaa
    13981 gcagacgcac acggagccaa ccgatgtcga accgctatgt ctatgtggac ggccgcttcc
    14041 tcgcctggca cgaagcgagc gtccatgccg aggatcgtgg cttccagttc ggcgatgccg
    14101 tctacgaggt gatcgagatc cgagacggtc ggctcgtcga cgagcgccgt cacatggacc
    14161 gcctcgagcg ctcgctcgcc gaactccgga tcgacgcgcc gatgagccgg gcggcctggg
    14221 cacccgttct gcgcgaggcg atccggcgca accgcgtgcg cgacggctcg atctacctgc
    14281 aggtctcgcg cggctgcagt ccgcgcgact tcctcttccc gccggcagga acgccgccga
    14341 cagtcgtcgt catcgcccgg catggctcgc gggcgaaggt tgaggcgacc gcggcacagg
    14401 gcatcggcat catctccatg cccgacaacc gctgggggcg ctgcgatatc aagaccgtga
    14461 tgctgctgcc ggcatcgatc gccaaggagg ccgccagggc aacgggcgcc aaggaggcgt
    14521 ggttcgtcga cgacaagggc ttcgtcaccg aaggcggctc gtccaatgcc tggatcgtcg
    14581 acaaggacgg ccgcgtcatc acgcgcaagt gcgacgaggc gctgctgcgc ggcatcacgc
    14641 gcacgacgct catcgatctc ctgaaaaagg acggcatcga actcgtcgag cgccccttca
    14701 ccatcgagga agccaaggcc gcgcgcgagg ccttcgtgac ctcggccacc aacatcgtca
    14761 tgccggtcgt cagcatcgac gggcggcctg tcggcaacgg tgccccgggt atcctcacgc
    14821 agcggcttcg cgagcgcttt cacagcgtgg ccgaaacctc ggcggcttag gcttagcgtc
    14881 gccggcgcat aaactgttgt ggcgatcatg cgcaattcag actaaaatac ggcttggtca
    14941 acttgtggcg tcgggcccgc gagaggcttg cctgcaggaa ataataatgg aagccgaaca
    15001 atggccgata gaccgatgag cctccaggac acgttcctga accatgtgcg caagcagaag
    15061 acgccgctga cgatttttct catcaatggc gtgaaattgc agggcattgt gacctggttc
    15121 gacaatttct gcgtgctgct ccgacgcgat ggtcactccc agctcgttta caagcacgcc
    15181 atctcgacca tcatgccagg gcagcctgta cacttgatgg atgccgatcc agccgatggc
    15241 gaatcgaaat aagcgttccg gcgacgaaat cgcgaccgac gacgacgatg ccacccatcc
    15301 cggcggtagc gccgacgagc ggccgagcga agcgtttgat cattcccatg tgacgacgcg
    15361 ggcgatcgtg ctcgtccccg tgctcaggtc tgcgagcgat cgtggcagga gccgtcggcg
    15421 ctcggggact gctcaagcgc cgggggcgcc tgtcgcccag cacagccccg aggatcgcct
    15481 cgccgaggcg accggcctcg ccgcagccat cgatctcgag gtcgcgtccg cgctcatcgt
    15541 ccccctcgcc ggcatccggc cgtccacgct gctcggctcg ggcaaggtcg aggagatggc
    15601 cgccctcgtg aagagcacag gcgcaggcct cgtcatcgtc gaccatgccg tgagccccat
    15661 ccagcagcgc aacctcgaga aggcgctcga gacgaaagtg ctcgatcgga cggggctcat
    15721 cctggagatc ttcggccggc gcgcgcgcac gcgggagggc cggctgcagg tcgagctcgc
    15781 tcacctcacc taccaaaaga gccggcttgt gcgcagctgg acccatctcg agcgccagcg
    15841 cggcggattc ggcttcatcg gtggcccggg cgagagccag ctcgagctcg atcgccgcct
    15901 catccaggac cggatcgatg ccatcaggcg cgagcttgcc gtggtcgtgc gcacgcgcga
    15961 gctgcatcgg gaagggcgcc gccgcgtgcc ttacccgatc gtcgcgatcg tcggctatac
    16021 gaacgccggg aaatcgacgc tcttcaatcg cgtgaccggc gcgagcgtcc ttgccaagga
    16081 tcaggtgttc gcgaccctcg acccgacgat gcgcgaagta aagctcgcga gtggccggcg
    16141 catcatcctc tccgatacgg tcggcttcat ctcggatctg ccgacgatgc tcgtcgccgc
    16201 cttccgcgcg acgctcgagg aggtgattga ggccgacctc gtcctgcatg tccgcgacat
    16261 ctcgcatgcc gagaccgacg cgcagcgcgc cgatgtcgag agcgtgctcg cgggtctcgg
    16321 catcgaggcg cagtcggccg cggcgccggt tttcgaggtc tggaacaaga ttgatcggct
    16381 tacagccgag gcacgatccg agctcggcgg tcaggcggag cgctcggagc gctctcccgc
    16441 gctcgtctcg gcggtgacgg gcgatggcat tgcggccctc ctgcaggcga tcgaccggcg
    16501 cctcagcgga tcggacgaga tcgttcgcct gatgatcccg ccgagcgaag gcggcgtcct
    16561 cgcctggctc cacgccaaca cggacgtcac gggacgcgag atcgccgagg acggcaccgt
    16621 caccgcgacc gtccggatcg atactgcgaa gaagggccgc cttctcggcc gcatcagcgg
    16681 aactgccgca cggatcgcgt gatccggctg gtcgaattcg gcgccgtgac tggcctccta
    16741 cacttgtccc ttcttcaccc aatcggccca ttcgggccgg tcgagcacga actgctcgtt
    16801 ggtgcgcgtg taggtgcccg agccgtgcat gcggccgacg agtttcatct tgggcgtgtc
    16861 gatgtagcag cgcttcggat cgatgacgaa gctgtcgtcg acatgtgcct gcacgacgcg
    16921 gccgatgacg atcgcctggt tcgggctgaa ggtcagcgag gtgtgcacgc ggcactcgaa
    16981 ggcgacgggc gagcccgcga tgcgcggcgg ggcgaccttc accgagggca ccgtgccgag
    17041 ccccgcgagc ttcaactcgt cgacaccggg cggcgcgtcg atgcaggtga cgttcatcgc
    17101 ctcggcgttg gcctcgttga cgaaattcac gacaaattcg gccgagttca aaatattatg
    17161 gccggtgtcc ttcagacgct tctctgcgtg tgcctggatg ccgatgacga tgatcggcgg
    17221 gttggtgccc atggcgttga agaagctgta gggcgcggcg ttgggtgtgc ctttgtcatc
    17281 gagcgtcgtc acccaggcga ttgggcgcgg cacgaccgtc gagatcagga tcttgtagcg
    17341 gtcgttctgc ggcatcgtct cgaagtcgaa ttccatggag gcctcggctc gcggggttcg
    17401 caggattgga gggaagaacg gaggtggtca ggccggcgcg acggtggcct tgacgacgcg
    17461 atcgatcgca atcagctcgc cgagcaaggc cccgagatcg cggagcggca ccatgttggg
    17521 cccgtcgctc ggggccttgt ccgggtcctg gtgcgtctcg atgaagagac ccgcgacacc
    17581 gacggcgaca gcggctcggg cgaggacagg cacatagcgc cggtcgccgc ccgaggatgt
    17641 cccctgcccg cccggctgct gcacggagtg ggtcgcatcg aaaatcacgg gcgcgccggt
    17701 ctcggccatg atggggagag accgcatgtc gttgtagccg aaactcgcgc cgcgctcggt
    17761 gacgaggacg ttgcggttgc cgctccccgt gatcttggcg accacgttct tcatgtccca
    17821 gggtgcgagg aactgcccct tcttcacctt gacgacgcgg ccggtcttgg cggccgcaac
    17881 gagcaggtcg gtttggcggc agaggaacgc cgggatttgc aggacatcga cgaccggcgc
    17941 gacgactgcg cactgcgcgg cctcatgcac gtccgtgacg acgggcaagc ccagcttctc
    18001 cttgatctcg gcgaacacgg cgagcgctcc atcgaggccg atgccgcgct tccccgaaag
    18061 cgacgtacgg ttcgccttgt cgaaggagga cttgtagacg agcccgattc cgagcctccg
    18121 cgatagctcc gcgagtgccg atgccatctc gagggcatgc gcgcggcttt ccatctggca
    18181 ggggccggcg aacaccgcga tcggccgccg gctcgcgaac gtcacattgc cgaccttcac
    18241 ctcaggtgcc ggcccggccg tctcgacctt ggtcatgctc gcccccagct catcgcttgc
    18301 cgatcacatg gttctggacg ccgccggccc cgagcttcgg ccgcacgctc gagatggtgc
    18361 cggagttgac gccggcccag ccgatctcgc ccgagaggcg gccgtattcg atcttggggc
    18421 agcggttcat aacgacgcga aggcccgcgg cctcggcttc caccgcggcg acctcgttcg
    18481 ccacggagag ctgcatccag atcaccttga ggccgagctt ctccttgaga gcgattgcct
    18541 cgcgcgtgac ctcgagtgct gcctccgagt tgcggaagat gtcgaccatg tcgaccttgc
    18601 cctcgatctc gccgagcgcc gccgcgaccg gctggccgag gatcttctgg cccgcgagac
    18661 ctggattgac cggtatgacg cgaaagccct tggaaagaag gtacttcatg gcgaagtagc
    18721 tcggccggtt ggtattggcc gaggcgccga ccatggcgat cgactggcaa tcgttaagga
    18781 tcgcggcgat gtaggcgtcg tcgtagcggt cgtggttcat ggcagcgtca ggcgcccttc
    18841 atcgtcgagc gggaagaacg ggttatgggc gacctcccag aggtggccgt cggggtcggc
    18901 aaagtagccc gaatagccgc cccagaacac cttgcagggc tgcttcacga gtgtggcgcc
    18961 tgaggcaacc gcgtgtgcga caaggtcatc cacttcggcc tcgctgcgcg cattgtgggc
    19021 gagcgtcacg cccgagaatt cctcgcccga gcccgcaacg acgccggcgt cctcggcgag
    19081 tgcctgccgg ccatagaggg cgagcgccat ggccccgagc tggaagaagg cgacattgtc
    19141 gttgctgctc gccgccgagg cggtgaagcc gagtgcctcg tagaactggc gcgacgcggc
    19201 gatatccgca acgccgagcg tcacaagcga aagccgcggc tcgaaacgcg tgtccgtcat
    19261 gggctagcac cccttccaat cgggcttgcg tttctcgatg aaggcgccga tgccctcggc
    19321 cgcgtcacgg tagagcatgt tctcgaccat cacctcgctc gcgtaggcgt aggcatccga
    19381 gaggcgcagt tcgagctgat cgtaaaaggc gcgcttgccg atggcgacgg ttgccgatga
    19441 ttttgatgcg atcagctccg ccatggccaa cgcctccggc atcacgcggt ctgccgccac
    19501 cgcgcgattg acgaggcccc agtccgcggc ctgctccgcc gtcatcagct cgccgaggag
    19561 cagcatctcc atcgcccgct ttctcggcac attgcgggag agcgccacca tcggcgtcga
    19621 gcagaagagg ccgatgttga cgccgggcgt cgcgaacttt gcatcggtat tggcgacggc
    19681 gagatcgcac gaagcgacga gctggcagcc ggcggccgtg gcggttgcct gcacggctgc
    19741 gatcaccggc ttcggtgagc gcacgatcgc ctgcatcatg gccgagcagc ggccgatgag
    19801 atcgcggaag taggcgcggc cgccgtcgga atcggagcgt cgcgccgtca tctccttcat
    19861 gtcgtggccg gcacagaatg caggccccgc gcctgtcagc acgatggcgc gaacatcgcg
    19921 ggccgcggat gcccggtcga tctcggcgtg caaggcggca atcatcgcct ccgagagcga
    19981 attgcgcgca gcggggcggt cgagcgtcag gatgagaacc ggtccccgcg tctcggactt
    20041 gagcgggcgg gcatcaacgg aggcatctga cttggtcgcg gtcgtcgtca tggctcatcc
    20101 ccgaacgcgg gtcgcgcggt gccgtggcgg ctcaccgcga tcgctgtgca tcttgcgtct
    20161 tgataggttg gctgcgctac gcaatgcaat gcgaattaat aggcttggcg gatcggcggc
    20221 cgatcgcggc gacttcatgt cgatttggag attggcgggg gccggcagcc aggcccgtgg
    20281 cgcatgtcca ctgggccgac cgccccgctc ggtctcagcg accagccccc ttggcatgag
    20341 tgctatcgca gtcgcggatc gcgcgcatcc tcaagtcggt tgagttggcg gcgaaacagt
    20401 cttactggtg tgctcagcga ggatcgcggg gaaatccgtg cgtattttct ggtgtgatgc
    20461 cgggtggcat cattcctgcg tcactcgcca gcatcgatca atgagatgtt cgacggagcg
    20521 caaccggctg gcgttgccgc caggcccttc cggtgcgcgc cgtctcacgt cgctgtgcag
    20581 cccgtgaggg catgccccgg tccgctgaac cgtcagcgac gtgcgcgccc attggtgact
    20641 ggatcgtgac ggcaatgcgg cggggaggca tgtctcgatt ggagttgnnn nnnnnnnnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncactct cgtacgcctc
    20761 gatggcggcg agccacacct cctctgctgc agcgagctcg cgccccttga gaccacgctc
    20821 gtgcacgagc ttctgggcct tccccgggtc gcgcgtgtag agcgtggaat cggagaggct
    20881 tgcatcgatc tttgcaatct ctgctgtaag tgcctcgacg cgcttttccg cggcttgcat
    20941 cgacttcttg aggggtgcga gctcgaggcg gcgctcggcg gccgcccggc gctgctcgat
    21001 gcgtccgggc tcgctcgtca acggcccctt cgcgacttgt cgcgtcttta cgccgcgctc
    21061 ggcgaggagc tccgcgcgat agctgtcgat gtcgccgtca tagggcttca ctgtgcccga
    21121 tcgcacgatc cagaggcggt cgacgcaggc gtcgacgaga tggcggtcgt ggctgatcag
    21181 gatcacggcg ccggggtact cgttgacggc ctggataagc gcctcgcggc tatcgacatc
    21241 gagatggttg gtcggctcgt cgaggatgag gagatgcggg ccgccgaagg ccgtgagccc
    21301 gaacagcagc cgcgccttct cgccgcccga gaggctctgg cacttcgtgt cggccttctc
    21361 cgacgagaag ccgagctggc cgaggcgtga gcgccgctcc gcctcgctcg ccgtcggcaa
    21421 aaggtcgacc atgtaatcgt agggccggcg tcccggcacg aggtcctcga gctggtgctg
    21481 cgcgaaatag ccgacgtcga tgcgcttggc gccgatcacc tcgccgcgct gtgctgtgag
    21541 gcgccccgcg atcagtttgg cgaacgtcga cttgccgttg ccgttggcgc cgagcagcgc
    21601 gatgcggtcg tcctggtcga ttctcaagtc gagattggcg agcacggcgc gatcgccata
    21661 gccgacggag acgccttcga gccgcagcag cgggctcgcc accaccttgg cggggcccgg
    21721 aaagtggaac ggcaccacgc ggtcctcgac ctgggcggcg atcggctgca tgcgcgcgag
    21781 ggccttcacg cggctctgcg cctgggcggc cttcgtcgcc ttggccttga agcgcaggat
    21841 gaagccctcg atgcggcggc gctcgtcgtc ctggcgcttc ttcagcttca gatcgaggcg
    21901 ctgcttctcg cgtcgcgcct cctcgaagtc gtcgtagccg cccgaataga gctctagccg
    21961 gccgcgatgc aggtgcagga tggcgccgac ggcgcggttg aggaggtcgc ggtcgtggct
    22021 caccatcagc acggtgtgcg ggtaagcctt gaggtagctc tcgagccaga gcgtgccttc
    22081 gagatcgaga tagttggtcg gctcgtcgag cagcaggatt tcaggcttca ggaacaggac
    22141 ggcggcgagt gccacacgca tgcgccaccc gcccgagagc tcccggcagg cccggcgctg
    22201 agcggcctcg tcgaagccga ggccggcgag gatctgcgcg gcccgcgagg gcgccgagtg
    22261 cgcctcgatg tcggtcagcc ggatctggat gtcggcgatg cgggtcgggt cggtcgccgt
    22321 ctcggcctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg gcggcaagca
    22381 cccagtcgat gagcgtgtcg tcgccgcccg gcgcttcctg cgcgatgtag ccgatccgcg
    22441 atcccctcgc gatcgtgatc gagccgaggt cgggggcgat ctcgccggtg atcaggcgca
    22501 gcagcgtcga cttgccggcg ccattgcgcc cgacgaggcc gaccttgtga ccggtcggaa
    22561 tggcaaccgt ggcgtcctcg atgatcggct tgccttcgat gcgataggaa agctcgttga
    22621 tgtgcagcat ggccgaggct gatagcggtg cggcggcgcc ttgtcaccat gtcccgcagc
    22681 atcaataccc attgccgacg cggccgcgat cgcgcgccgc gataagcgcg ccgcaggacc
    22741 ggaattcgcg gccatcgaaa ctggcggcta tagtgacgat ccatcctggc cgacggtcgg
    22801 acctgcgcgg caacgtgctt aacttgacgt taacgtcaat tacgcctagt cgtgccgagc
    22861 gtcccggcgg atgatggggg cgacgagaga acgccgaagg tcaacctgga ggcaggcagc
    22921 atgtccacgt ttctcttcct tgcggtactc atcggtctcg tgttcgcgct cgccatgaag
    22981 cgggcgccga tgtggctctg ggccattgcc gcggcggcct tcgcctatgt gacgcaagca
    23041 ggcctcttca acggtgagtt cgagccgcgc tggccgggct ttctcggcgt gatcgcctgg
    23101 cttccggtgg cgctgcttgc gcttcttgcc gtgccgccgc tgcgtcgggc cgtcctcgtc
    23161 gcccctgcct tccgcatgat caagggcatc ctgccgcgtg tctccgagac tgagcagcag
    23221 gcgctcgacg ccgggaccat cggtttcgac gccgagcttt tctcgggcag gcccgatttc
    23281 ggcgtgctac gtgcggtccc tggcatcgag ctctcagccg aggagaaggc cttcctcgac
    23341 gggccgacgg aagagctctg ccgcatgatc gacgactgga aggtgcgcca cgccgagaag
    23401 gagatcccgg agcccatctg ggacttcgta aagcgccacg gcttcctcgg catgctgatc
    23461 tcgaagtcgc acggcggcct cggcttctcg gcgcaggcgc agtcgctcat tctcggcaag
    23521 atcgcctcgc gctcgcccga tgtcgtgacg attgtcatgg ttccgaactc gcttggcccg
    23581 ggtgagctca tcgagaagta cggcaccgac gcgcagaagc accacttcct gccgcgcctt
    23641 gcgaagggcc tcgaggtgcc ctgcttctcg ctcaccggtc cgacctccgg ctctgatgcc
    23701 gccacgatgc gcgacatcgg ctgggtgacg aatggcatgc acgagggccg cgagaccgtc
    23761 ggcattcggc tttcctggga caagcgctac atcacgctcg gccccaaggc gacgctcgtc
    23821 ggcctcgcct tccggctgtt nnnnnnnnnn caacctgctc gggaagggcg aggacatcgg
    23881 catcacggtg gcgctggttc cggcgaacca tccgggcgtc cacatcggcc gccgccacct
    23941 gccgtcgggc gccgctttcc cgaatgggcc caactggggc aacgacgtct tcatcccgat
    24001 ggactgggtg atcggcggcg agaagatggt cggccagggc tggcgcatgc tgatggagtg
    24061 ccttgccgcg ggccgcgcca tctcgctccc ctcgtccggt gccgccggtg ccaaggcgat
    24121 gttgcgcttc tcgtcggcct atggccgcgt gcgcaagcag ttcggcctgc cggtcgccaa
    24181 gatggagggc ctcgaggagc cgctcgcccg gttggtcgag acggcctacg tcaacgaggc
    24241 gggccgcgcg gtcacggccg ccatggtgag ccgcggcgag aagccttcag tcatctcggc
    24301 catcatgaag taccagtcaa cggagcgcat gcggcgcgcc gtcaacgacg ccatggatct
    24361 gcacggcggc cgcggcatct gcgacggtcc gtcgaactat ctgcagtccg cctaccagat
    24421 ggtgcctgtc ggcatcacgg tcgagggcgc caacatcctg acgcgctcgc tgatcacctt
    24481 cgcgcaaggg gcgctccgtt cgcaccccta cctctacaag gaggtgcagg cctgtcagga
    24541 ccccgatacg tcgaagggct tgaaggcatt cgaaagcgcc tttctcgggc acctctcctt
    24601 ctcgatgtcc aacgccttcg gcgccttctt ccacaacgtg accggcggca tctttggcaa
    24661 ggtgccggcc aacgccggcg acaacgcgga gtggtatcgc cagctctggc gctgctcgcg
    24721 gagcttcgcc ctcgtcgctg atctgacggt ggcgctcctc ggcggcggac tcaagaccaa
    24781 gcagaagctc accggccgca tggcggacgc gctctcggaa ctctatttcc tcgcctgcat
    24841 cctcaagcgc ttcgaggacg acggccgccc ctcggcggac cgccacatcg tcgcctactg
    24901 cgcgcagaac tgcctctacc gcttccagga gtcgatgcgc ggtgccattc acaattttcc
    24961 gattgcctta gctcgcatga agatgcgcgc gctcgtcttc ccgctcggcg cccgttacaa
    25021 gccggcgccg gaccgcctcg gcacgaagat cgtttcgctc gtactcgagc ccggcgaggt
    25081 gcgagatcgc ctgacacgct acatctacgt ctcgaaagat ccgaaggacc cgacgggcgt
    25141 gctcgaggtc gccctcgaaa aggtgatcgc tgccgagggc gccgagaaga agctcgagcg
    25201 cgcgatccgc gcgggcacgg tgcgccgctt ccatggcatc gattggatcg ccgacgcggt
    25261 cgcgaaaggc gtgctcaccg aggaggaagg ccgcggcctc aaggaagcgg aggcactgac
    25321 tgatcgcgtc atcgccgtcg accacttcga tccggtcgag gtgaagccca attacatgac
    25381 ggcgggccac aacgccgccg ccatgacgag ccgtgcggcg gagtgaccgg cggggcctgc
    25441 gatctcgggc aacagtgcga gcggtcgcgc gccgctcgcc ggcgcctcaa tgcttcgcat
    25501 cggagccgtt cttcttccag tagacctcgc aacggccgta acactgccaa aacttcttgc
    25561 tgcccgaccg gttgtgggcg ccgcaccagg ccgtgcagcg ggccaagcct tcgtcgaccg
    25621 tcagccgttc cctggcgacg gtttcagagg ctggcagtgc gagcaggccg agcgccaaag
    25681 cgagaccact cgcggcaaga agcatcggct tcgttacgga tttcatgtcc ggctcctgtg
    25741 tgtatgtcgt tgcgcgccga gtggcgacat atgaactatg tccactccag aacgaaaaat
    25801 cgcgatgccg gcattcgatt ccgtggaaaa aacccggcgg tggctaccgg cggcctcggc
    25861 tcgacgaggc gcggcgcgac aggtaagatc gacggcgaat ttcgaatgac tcgcgagggc
    25921 tcgctcggcg ggctcggatt tcggcattcg cgatgttccg catggagccc gcgccgtatc
    25981 cggggcatgc gccaggatcg atcgagcaca cctcgatgtg caccacctca accgctccgg
    26041 agccgaccat ggccgacctc gctcacctga aggactggcg ctacgcggtc gacggcgagg
    26101 gcatcgcctg ggccgtgttc gatcgcgctg gtgaaagtca gaattcgctc ggccgccggc
    26161 cgctcgagga gctgattcaa atcgtggaag cggtcgaggc gggcgccaag gacaagagca
    26221 tccgcggcct cgtgttcatc tcgggaaagg agaaaggctt catcgtcggc gccgacgtgc
    26281 gcgagttcga gcagttcagg acggcggccg aggtcgagga ggcgatcaag cctgtccttg
    26341 ccatgctcga ccgcatcgag gcgctgccgg tcaccgtggt cgcggcgatc catggcttct
    26401 gcctcggagg tggcctagaa ctggcgctcg cctgccacta tcggatcgcg acgcgcgacg
    26461 atggcacacg ggtcgggttc ccggaggtga agctcggcat cttcccgggc ttcaacggca
    26521 cggcccgctc gatccggcag gcgggtccgc tcgccgccat gacgagcatg ctgtcgggtt
    26581 ccatgatccg cgcatcggcc gcaaagggca tgggcctcat cgacgagctc gtgccgacgc
    26641 cgctgaacct gcgctgggcg gcgcgcaagg cgatcgacag gaagcggcgc tccgaagcga
    26701 agggcatgct gcagtcagcg ctccgccagt ggccgctgcg tggcgtgctc gcctcgcgca
    26761 tgcgcaagga ggtcgcgggc aaggcgcggc ccgagcacta tccggctccc taccgcctga
    26821 tcgatctctt cgaccggcac ggcggcaacc tcgacgcgat gaagcgcgcc gagacgagtg
    26881 cctttgcccc gctgatggtt tccgataccg cccgcaatct ccgccgcgtc ttccatctga
    26941 tggaggcgat gaaagccgag gcgccgcggg atctcggctg gaggccgacg cgcgtccacg
    27001 tcatcggcgc cggcgtgatg ggcgccgaca tcgccggctg gtgcgtggcg agcgggctcg
    27061 tggccacgct ccaggacttg agtgaggagc agatcaagaa ggggatcgaa gcccagaaaa
    27121 agacgctcga gcgcaagttc cgcacgcgcc ccgagcgcga ggcggcgcgc gcgcgtctga
    27181 tcgccgaccc gaagggcgag ggcatcgagc gggcggacgt catcatcgag gcgatcgtcg
    27241 agaagctcga ggtgaagcag agcctcttca gaggtctcga aggccgcctg aagcctggcg
    27301 ccgtgcttgc gaccaatacc tcctcgatca tgatcgagga catcgccgcc ggcctcgcca
    27361 acccggcgcg actgatcggc atccatttct tcaacccggt tgcgcagttg ccgctcgtcg
    27421 aggtcattcg cggcgcgggc tcggccgaga gcgagctgcg caagggctgc gccttcgtga
    27481 ctgcgatcga caagctgccg ctgatcgtca agagctgccc gggcttcctc gtcaaccgtg
    27541 tgctcgcgcc ctacatgctc ggcgcgatgc agcgcctcga gcagggcgag agcaaggaga
    27601 agctcgacga ggctgcgcgc atcttcggca tgccgatggg gccgatcgag ctcgccgaca
    27661 gcgtcgggct cgatgtctgc gcgcatgtcg gccgcatcct gaagctggcg ccgcccggcg
    27721 aaagtacgct cgaccggctc gtcgccgcga agaagctcgg caaaaaatcg ggcgagggct
    27781 tctatgtctg gaaggacggg aagcccgcga agggcgaggt caaggccgag accgccgaac
    27841 tcggccggct cggccacgag ctcgtcgccc cgctggttgc ggaatgcgag cgctgccgcg
    27901 acgaaggcat cgttgcgagc gccgacatgg tcgacgcggg cgtcatcttc ggcacgggct
    27961 ttgcgccgtt ccgcggcggc ccgctgacct acgctgcggc ccgcaagctt gctcccgccg
    28021 agacgcgggt cgcggcggag tgagagacgg cgcaaaagta tttgccacgc catctacctg
    28081 tggagggcgc atatgacttc ggcagtcgaa acggtatccg atggctccac tgccctgacc
    28141 cgcgagcatg tgctgtcgct gctccgcgag cacgaagcgg cagttcaccg atatggtgtg
    28201 aaatcgctgc atctctacgg ttcggccgca cgggacgagc tgacgccaag gagtgacgtc
    28261 gacttgttcg tcgattgcga cgacgtcaac gcgttcggtt ttgtcgagct tctcgatctg
    28321 cggggcttct tgagggactt gttaggccgg aatgtcgacc tgacgagtcg ggccggcctg
    28381 catcgcctgc tcagggcgaa aatcgaacag tcgagtcctc aggtattctg atatggccaa
    28441 gcctgcgacc gaggtccgta tcagggacat gttagacgcc atctacaccg ctcgggagct
    28501 cagaagcggc atgaccgtcg acgtgtatgt gtcggcgatg gcgcgcggcc agaggcgcgg
    28561 cgtcgagcgt tgggtcgaga tcacctccga ggcgagcaga catctacctc gcgagctgac
    28621 cgacgcattt cccggcattc cgtggttacg catccgtgac gtcggtaacg tcattcggca
    28681 cgcccattcg tccgtcgacg accagatgat ttggcgcatc gcgacacaac atctcgatga
    28741 gccggagtcg gtgttgcagg tgatgctggc cgggctcgcc cctgacgttg actgctgaat
    28801 gccacccaac tcaaggaagg cagccgaatg acccccactc tccgccgtgt cgccattgtc
    28861 gggggtgtgc gcatcccctt ttgccgctcg aacacgctct acgccgacct ctcgaatctc
    28921 gacatgctga cggcgacgct caacggcctc gtcgagcgct acaagctcgc tggcgtgcac
    28981 atcgacgagg tcgtgggcgg cgccgtcgtc acgcattcgc gcgactggaa cctcgcccgc
    29041 gaggcggttc tcggcaccaa gctcgcccgc tcaacgccgg gcatcaccat gatccaggcc
    29101 tgcgggacga gcctgcaggc ggccatgggg atcgccgcca agatcgccat cggccagatc
    29161 gagagcggca tcgcctgcgg gtcggacacg acgtcggacg cgccgatcgt gctccggaag
    29221 tcgttcgccc agcagctcgt gaaggggcag caggcggcga agaccttggg cggcaagatc
    29281 aagctcgcca tgaccgcggc gaagcctgcg gccatggtac tcgagacgcc ctccaccgcc
    29341 gagccgcgca cgggcctctc gatgggcgag cattgcgagc tcatggccaa ggaatggcgc
    29401 atcccgcgcg ccgagcagga cgagctcgcc gtggcgagcc accagaaggc ggccgaggct
    29461 taccggacgg gatggatgga cgacctcctc gtcccctgtg ccggcgtctt taaggacaac
    29521 aatctgcgcg aggacgcctc ggtcgaaaag atggcggccc tgcggaccgc gttcgacaag
    29581 acggagaaag gcacgctgac cgcgggcaac tcgacgccgc tcactgatgg cgcctcctcg
    29641 gtgctgctcg cgagcgagga gttcgctcag gcgcgtggcc tgccgatcct cgcctacctc
    29701 gtcgatgccc agacctccgc caatgatttc gccgcaggcg aaggcctgct gatggcaccg
    29761 acgatcgctg tctcgcgcct tctcgatcgg caaggaatgc ggcttcagga cttcgcgacc
    29821 tacgagatcc acgaggcctt cgcggcgcag gtgctggcaa cgctccgcgc ctgggaggac
    29881 cccgcctact gcaagcggta tctcggcaag gatgcgccgc tcgggtccat tgatcggacg
    29941 cggctcaacg tgaaaggctc gtctctcgcc ttcggccacc ccttcgcagc aacgggtgcg
    30001 cgcgtcatgg cgaaccttgc caagctcctc aacgtcaatg gcggccgcgg gctgatctcc
    30061 gtctgcaccg ccggcggcat gggtgtcgcg gccatcctcg agggagcgcg gcgctgacga
    30121 cacc
//
LOCUS       DAORNH010000017        29137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1214_length_29137_cov_2.668420, whole genome shotgun sequence.
ACCESSION   DAORNH010000017 DAORNH010000000
VERSION     DAORNH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 29137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29137
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_29137_cov_2.668420"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /gene="carB"
                     /locus_tag="PK264_03260"
     CDS             <1..1483
                     /gene="carB"
                     /locus_tag="PK264_03260"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRD74947.1"
                     /translation="ETIMVNCNPETVSTDYDTSDRLYFEPLTTEDVLEILNVEMSNGT
                     LAGVIVQFGGQTPLKLAETLASAGVPILGTSPDAIDLAEDRDRFKALIDKLKLKQPES
                     GIARSPEEARAVAERIGYPVVIRPSYVLGGRAMEIVSDSAEVERYVQRLSQTLDRPSE
                     LVVSDRRPLLIDRYLSDAIEVDVDCLADRQTTFVAGIMEHIEEAGIHSGDSACSLPTH
                     SLDSSIVVRLEKQTRDLALALGVVGLMNVQYAVKGREIYILEVNPRASRTVPFVAKVI
                     GQPIAGIASRVMAGEPLASFGLKPKRLTHVAVKEAVFPFARFPGVDPVLGPEMRSTGE
                     VMGIDRNYALAFAKSQLGSGTVLPSAGAVFLSLRDSDKDAVLGAMRELSDIGFRLVAT
                     RGTRRHLESHGIPCELVNKVLEGRPHIVDLMKSGEIQLVINTTEGAKALADSKDIRRT
                     ALINHIPYYTTLAGGLAAAEAIKALRADILDVAPLQSYVGKPT"
     gene            1653..2126
                     /gene="greA"
                     /locus_tag="PK264_03265"
     CDS             1653..2126
                     /gene="greA"
                     /locus_tag="PK264_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRD74948.1"
                     /translation="MEKVPMTLEGFKKLEEELHRLKAVERPRIIQAIAEARALGDLSE
                     NAEYHSAKEAQGLNEARVADLEDKVSRAEVIDPAKMSGDTVKFGATVTLEDEDTGEKV
                     KYKIVGDPEANVREGRISISSPIARALISKSKGETADVTTPKGSRSYEILKIEYK"
     gene            complement(2133..>2329)
                     /locus_tag="PK264_03270"
     CDS             complement(2133..>2329)
                     /locus_tag="PK264_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRD74949.1"
                     /translation="GYVTLVPDTEVIYKVTDLYAPEHEGGLRWDDPALAIDWRITADR
                     AILSPKDRVLPTLAALPPVF"
     assembly_gap    2330..2342
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2343..2684)
                     /locus_tag="PK264_03275"
     CDS             complement(<2343..2684)
                     /locus_tag="PK264_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HRD74950.1"
                     /translation="MDVRTLDIPDVKVLAPRIFRDERGFFSETWSAATLAKAGIEATF
                     VQDNHALSREAGVVRGLHFQRPPRAQDKLVRVVRGAILDVAVDIRKGSPTYGQHFAVE
                     LSADNWRQLYVP"
     gene            complement(2698..3597)
                     /gene="pssA"
                     /locus_tag="PK264_03280"
     CDS             complement(2698..3597)
                     /gene="pssA"
                     /locus_tag="PK264_03280"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HRD74951.1"
                     /translation="MDPIDPADLRDPTGAPSRRRLFRRRKIPVRLLVPNFFTLLSLCA
                     GITAMRMAIEKQFEWALALIVFAALLDGVDGRLARALKSQSRFGAEFDSLADFVNFGV
                     APAILVYTWGLGDFKRFGWIAVLLFACGMALRLARFNSMLDVDKPRWQSNYFTGIPAP
                     AGALTVLLPLYLEGLGIPGFDPKAYPAVITVYTLALAFMLVSTIPTFSGKLLGERIPR
                     ELVMPILFGVVLVVALLVTYPYVTLAIITLGYLALIPFGYVKFQKEYAAWKAATASAP
                     ASEPLSGTNVVTLSPPSQSDMKH"
     gene            3770..>4759
                     /gene="guaA"
                     /locus_tag="PK264_03285"
     CDS             3770..>4759
                     /gene="guaA"
                     /locus_tag="PK264_03285"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRD74952.1"
                     /translation="MTDTILILDFGSQVTQLIARRVREAGVYSEIVPFSQGEVAFDRL
                     RPKGIILSGGPASVYWSEAPRVPRRLLEAGLPVLGICYGNQTMVTELGGNVEGGHNAE
                     FGRADIDIVEDCALFKGVWSKGERDHVWMSHGDKITRIPDGFRVVARSDNAPYATIAD
                     ERRRLYGVLFHPEVAHTPRGAALIRNFVHNVAGCRGDWTMAAFRASAIERIRRDVGKS
                     RVICGLSGGVDSAVAAVLIHEAIGDQLTCIFVDHGLLRLNEGSEVVKLFREHYNIPLV
                     HVDASRQFLTALDGVSDPERKRKTIGALFIDVFEAEAKTIGGAEFLAQGTLYPD"
     assembly_gap    4760..4769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4770..5327
                     /locus_tag="PK264_03290"
     CDS             <4770..5327
                     /locus_tag="PK264_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HRD74953.1"
                     /translation="VIESVSFTGGPSVTIKSHHNVGGLPARMNMKLVEPLRELFKDEV
                     RALGGELGLPPAFIGRHPFPGPGLAIRIPGAITPAKLDILRKADAIWLDEIRKAGLYD
                     TIWQAFAVLLPVQTVGVMGDARTYEAVCALRAVTSTDGMTADYFPFPHDVLGRTATRI
                     INEVRGINRVVYDITSKPPGTIEWE"
     gene            5402..5659
                     /locus_tag="PK264_03295"
     CDS             5402..5659
                     /locus_tag="PK264_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRD74954.1"
                     /translation="MTITTISSRKFNQDAGGAKRAAANGPVIITDRGRPAHVLLNYAE
                     YLRLTRQGPSLLDVVAMPGGEHIEFEAPKLKGPLSKSVDLD"
     gene            5659..6081
                     /locus_tag="PK264_03300"
     CDS             5659..6081
                     /locus_tag="PK264_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD74955.1"
                     /translation="MFLLDTNVVSELRNMSRANPSVRALAARTPAQLLWMSTISVLEL
                     EIGVLRAERRDPPQGVLLRRWLEGQVLVQFEERLLMVDAVVARACAGMHVPDPRPERD
                     ALIAATAKAFGLTIVTRDTADFEPMGVPVLDPWSTEQS"
     gene            6388..7377
                     /gene="ppk2"
                     /locus_tag="PK264_03305"
     CDS             6388..7377
                     /gene="ppk2"
                     /locus_tag="PK264_03305"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRD74956.1"
                     /translation="MPPVQPTPSTKPPATNGSALSADGPSLMPLAADPDNKTSIAQRR
                     RDPDYIRRAFESGEYPYQTRLRTAEYEAAMAELQVELLKAQKWVKESGERIVILFEGR
                     DAAGKGGTIKRFMEHLNPRGARVVALEKPTDRERSQWYFQRYINHLPAGGEIVFFDRS
                     WYNRAGVERVMGFCNPNDYLEFMRQCPDLERMFVRSGIRLFKYWFSVTRAEQLRRFKA
                     RETDPLKQWKLSPIDLSSLDKWDDYTEAKEAMFFYTDTADAPWTIIKSDDKKRARLNA
                     MQHFLAILDYPAKNQALIRGPDPLIVGSSAHVIGRDEHILGKTLHPEKRRRHT"
     gene            complement(7399..8403)
                     /gene="meaB"
                     /locus_tag="PK264_03310"
     CDS             complement(7399..8403)
                     /gene="meaB"
                     /locus_tag="PK264_03310"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRD74957.1"
                     /translation="MKRSDAPLVRELADHIRAADRAAIGRAITLIESRRADHQATASA
                     LLQELIADTGRAHRIGITGVPGVGKSTTIDQLGMNLIAAGRRVAVLAVDPTSTRSGGS
                     ILGDKTRMSRLSQQGGAFIRPSPSSGTLGGVTRKTRETMAVLEAAGFDVVIVETVGVG
                     QSEVAVAAMVDVFVVLLLAGGGDDLQGIKKGIIELADVLAINKADGDNVDRATRAAAE
                     YMAALNILTPAGSLWLPPVLTISGRDDKGLDKLWQTLEQHRAKMEGTGTFETRRRSQA
                     VTWMREMLADRILAAVAADPRIAARIPALEADVQSGRLAPTRAVDEILRAVGMLSERT
                     "
     gene            8616..8741
                     /gene="ykgO"
                     /locus_tag="PK264_03315"
     CDS             8616..8741
                     /gene="ykgO"
                     /locus_tag="PK264_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRD74958.1"
                     /translation="MKVRNSLKSLRGRHRDNRVVRRKGRVYVINKTNRRFKARQG"
     gene            8842..9888
                     /gene="trpS"
                     /locus_tag="PK264_03320"
     CDS             8842..9888
                     /gene="trpS"
                     /locus_tag="PK264_03320"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRD74959.1"
                     /translation="MSQKSPPLPRVFSGMQPTGESPHLGNYLGAIVNWVEMQKTHECI
                     YCVVDMHAITQWQDPAGLKDSIRAVAAAYMAAGLDPTRCILFNQSQVGEHAELAWIFN
                     CVARIGWLNRMTQFKDKAGKDREAASVGLYAYPNLMAADILVYRATHVPVGEDQKQHL
                     ELSRDIAQKFNNDFADGIARLGYTGGFFPLPEPMILGPAARVMSLRDGTKKMSKSDPS
                     DLSRINLSDDADTIARKLQKAKTDPAALPSEEAGLDNRPEADNLVGIYAGLAAKSKSD
                     VLRDHGGAQFSTFKKALIELAVARIGPISAEIARHTADPAEIDRVLRDGADRARAIAR
                     PIMDDVKDIVGFLR"
     gene            complement(10531..11022)
                     /locus_tag="PK264_03325"
     CDS             complement(10531..11022)
                     /locus_tag="PK264_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HRD74960.1"
                     /translation="MTQPHRSIRRWRIALILPIVGTLAAGCAGDGSKQDTGLVVGAVA
                     GGILGNQVGKGAGRIVATATGAFIGGVIGSEIGRSMDRQDRLLAQDAELRALERGTSG
                     TPVGWRNPDNGRYGEVVPGAPYRRGVHHCRDYAHTVYIEGRMQTMRGTACRNPDGTWR
                     AVS"
     gene            complement(11175..12629)
                     /locus_tag="PK264_03330"
     CDS             complement(11175..12629)
                     /locus_tag="PK264_03330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD74961.1"
                     /translation="MKLNSLYVKLLLSGLAWTIIVLPLAGFLIDRRHKQEVLDSFDDK
                     LFELLLQMHLSSENLSGIPVPARFANDDNFQYRDSGWYWQITPKDGAGGKRILSESLA
                     GKPIRLPSELGAPLVTKTTSYQSIPGVEIPLVPGVPRFNDQFTGTFVRLADAKGPGSE
                     PIRIIEAILNPSPQHKDIWYSYAIAGRLDQTHADIARFRRELVWAFGFVGLALAVLMF
                     ASFRFILRPLRQIEDGLGAIRSGDAQRLEALLPAEVEPLREQVNALIVSNQEIIERAR
                     RQVGNLAHALKTPLAVIINEANESKTPFAIKVSEQAAVMRDQVNRYLDRARIAALVGV
                     VVRSTDVKPVADSIIAVLEQLHDDKALDIVATGDASARFQGEKQDLEELIGNLLDNAC
                     KWARSRITLDMQVRSAEGAKRLVLTVDDDGKGLTAEQRAQGVKRGRRLDETKPGSGLG
                     LTIVSDLIETYRGAFQLDESPLGGLRVIVELPAT"
     gene            complement(12619..13290)
                     /locus_tag="PK264_03335"
     CDS             complement(12619..13290)
                     /locus_tag="PK264_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD74962.1"
                     /translation="MRALVVEDDPDLNRQLVTALTAEGFAVDTASDGEEGFFLGETEP
                     YDIVILDIGLPKMDGISVLEQWRRADRNMPVIILSARDRFSDKASGINAGADDYVGKP
                     FHMEELLARVRARVRRAAGHAKPVIECGALRLDTKTARVTYDGTLVKLTSHEYRLLAY
                     LLHHNGRVVSRTELVEHLYEQDFDRDSNTIEVFIGRLRRKIPGDLIKTVRGLGYRLAQ
                     DGDEA"
     gene            13502..14005
                     /locus_tag="PK264_03340"
     CDS             13502..14005
                     /locus_tag="PK264_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD74963.1"
                     /translation="MHQQPIPGLIGLDHVGLTVPDVDRATAFFVDVLGAEALYDIGPF
                     TDPAEWMASHLARRADARIERMRVLAVANGPMLELFELSGAALETAGVHLAFYVEDME
                     AALGAVKAHGAVIQSGPVEMTEGPSAGLTWLYFTAPWGLQLELVSYPRGIAALRERKT
                     PVWRPRR"
     gene            complement(14010..14312)
                     /locus_tag="PK264_03345"
                     /pseudo
     CDS             complement(14010..14312)
                     /locus_tag="PK264_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
     gene            14438..15586
                     /locus_tag="PK264_03350"
     CDS             14438..15586
                     /locus_tag="PK264_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74964.1"
                     /translation="MANYRTLLERLLLAACLVSAPSFGRAEGGDEAGPVALRNIGAPR
                     ALANKLPRSEADQALLRGWPLYRTERGQEAFNAAMATLKATEGAPPAASTFRGCVGLA
                     CRLALPRIGMNGWLPAGRIWVSPTEYVLIAHSSRFRDGQRMRRRMAREMRYLVLHEFH
                     NGSRNTDVYDTISSHSRAVFVPLYMSRQGIDAHGHAFVVVVQVAPYDVASIHASNFGN
                     AGPGIEVAKNYGDPLDPVQSLAGILVATMIKEAAPQLKVVHHRGSEGRAMLEAYDNRV
                     ARVASRAGLAPVALPFVPAPPARLASVSAGTVASLVRRGTGSVVVPVADRGILYGRTR
                     VAGIAPRPMLIEPVAPVTADDLLLPPRLIGPILPAVRPSVIGHGAPVN"
     gene            15653..16585
                     /locus_tag="PK264_03355"
     CDS             15653..16585
                     /locus_tag="PK264_03355"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRD74965.1"
                     /translation="MAKLTADAKGVYVIAATPFKEDGAIDHASIETLTEFYIRSGVTG
                     MTILGVMGEFQKLSESETLDVARRFISCAKGRVPVIVGVSNPGTTQLLKVSRAAMELG
                     AAGVMVMPTPGLRTDEAVSAYMGSVLTGLGAQIPVCVQDYPQLTGVYFSADCFLRLVD
                     AYPQIVMFKHEESPGLRKLSAVRRATDGKTRRRISILCGNGGIHLPQEYARGADGAMT
                     GFAYPDMMARMHAIFEAGKRGEAEDLYDLYLPILRHELQPGIGLALRKEILRRRGAIA
                     SAAVRAPGPKLDADDIAELDALLARLEAKLPIEP"
     gene            16887..>17700
                     /locus_tag="PK264_03360"
     CDS             16887..>17700
                     /locus_tag="PK264_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRD74966.1"
                     /translation="MATQPYLRLICSTAAALALGGCMVHPVALTEDEIGARARVNLAT
                     LQTGDAPVSGAIDLYEAMARALKFNLDARVEAAQTALRLAELDLSHWSLLPNAVANSG
                     YAGRNNYNASSSFNVITNTPNAFVSTSTSQEKRILSGDIGFSWNVLDFGLSYIRARQS
                     ADKVLIAGELRRKIILRLMEDVRTAYFRAVSAERMMRKLMVLDARARVALAGSRTVYK
                     DRQTSPITALTYERELVEIRRTIQELARDLSVAKAQLAALMNLKPGTQFALVV"
     assembly_gap    17701..17714
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17715..>18409
                     /locus_tag="PK264_03365"
     CDS             <17715..>18409
                     /locus_tag="PK264_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRD74967.1"
                     /translation="AASPALGLGAAEMIAIAQRNRPELKEIAYQRRINDQEMHAALLE
                     LLPSFNTYVATNFDSNAFLLNQHWASWGAKASWNLLKVFQYPAKREVVEQQDEVLRQR
                     ELAITMAVITQVHVSRARYLLARRELGIAAEYLSVQTRLVGQMRTEAKADRISEQTLI
                     REEMNTLVAEIRHDITYATLQSAYATLFASIGLDPYAEASTSDVPLKELAAQLRSLWF
                     ERGQFRKRAWDAVA"
     assembly_gap    18410..18420
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18787..19821
                     /gene="coxB"
                     /locus_tag="PK264_03370"
     CDS             18787..19821
                     /gene="coxB"
                     /locus_tag="PK264_03370"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HRD74968.1"
                     /translation="MIERARSVLGRVAAHVSASVTLALFAMITGVTGAAAQKLPDGMG
                     QPFPWQVGLQKPATEVAREINIFHDRVTILITVITIFVLVLMLYVMFAFREGRNPSPS
                     RTTHNTFLELAWTIVPVIILIVIAVPSFKLLYLQYSFPKPDLTIKAIGNAWYWEHEYP
                     DHGNFKVTSNMVTDEEVIKTKIGADEFSKRYKGLAGIERDKRLYADAQPIWAERKEPR
                     LLTVNNEIVVPLGKVVHLLVTANDVIHNWTIPSFGSKVDAVPGRVTATWFRPEVAGLF
                     YGQCSELCGKDHAFMPIAVRVVPDAVFAQWADAMKARDRRRAREILQRAEAEANGTTV
                     AAVSPSSSGK"
     gene            19990..>20605
                     /locus_tag="PK264_03375"
     CDS             19990..>20605
                     /locus_tag="PK264_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRD74969.1"
                     /translation="MASKAHADHGDGHDHAPYGITRWLYSTNHKDIGTMYLIFAMMAG
                     VIGGFLSVMMRLELQEPGLQYFSNTHLFNVFVTGHGLIMVFFMVMPAMIGGFGNWFVP
                     LMIGAPDMAFPRMNNISFWLLPASFALLLLSMFVPGAGTCEIGKDMSGCGVGAGWTVY
                     APLSTRTGHSGPAMDFAILSLHIAGASSILGAINFITTIFNMRAP"
     assembly_gap    20606..20762
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20763..>21126
                     /locus_tag="PK264_03380"
     CDS             <20763..>21126
                     /locus_tag="PK264_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRD74970.1"
                     /translation="YQHLFWFFGHPEVYILILPGFGMISHIVSTFSRKPVFGYLGMAY
                     AMVAIGVVGFVVWAHHMYTSGMSVNTQAYFVFATMVIAVPTGVKIFSWIATMWGGSIR
                     FTTPMLWALGFIFLFTVGG"
     assembly_gap    21127..21189
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21190..21628
                     /locus_tag="PK264_03385"
     CDS             <21190..21628
                     /locus_tag="PK264_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRD74971.1"
                     /translation="AHFHYVLSLGAVFAIFAGWYYWFPKMSGYMYSETLGKAHFWITF
                     VGANVLFFPQHFLGLAGMPRRYVDYPEALAGWNKVSSFGSYMTAFGTIVFFIGVFLAL
                     SRKEKAQANPWGEGATTLEWTLPSPPAFHSFEKLPRITAGDHH"
     gene            21795..22679
                     /locus_tag="PK264_03390"
     CDS             21795..22679
                     /locus_tag="PK264_03390"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRD74972.1"
                     /translation="MGGEVGDYIALMKPRVMSLVVFTALVGLVVAPGSLHPALALIAI
                     VCIAVGAGASGALNMWYDADIDARMARTAGRPIPAGRISPDEALTFGIVLSLASVVTL
                     GVLVNWVAGILLALTIVYYTVVYTMWLKRRTPQNIVIGGAAGAFPPMIGWAAVSGGVS
                     LESIVLFLIIFMWTPPHFWALALFRSGDYERAGVPMLPVVSGAAETRRQIVIYSVLLV
                     PLAVVPSFIGLGGLAYGVVSVGLGGWFLALALQLYRAGDCPGSLTIAKKLFGFSILYL
                     FALFAVLLVEALVARGGA"
     gene            22676..22936
                     /locus_tag="PK264_03395"
     CDS             22676..22936
                     /locus_tag="PK264_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74973.1"
                     /translation="MSTVARDGDRDGLRRQRSRSIAIALSLVALVIVFYVATLIHLGP
                     NALNKSAYNRPESGKVKIVTDPTGKSESFKGVDPDCKKAGTC"
     gene            22930..23508
                     /locus_tag="PK264_03400"
     CDS             22930..23508
                     /locus_tag="PK264_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HRD74974.1"
                     /translation="MLMSRDMRVAAICASIAFGMVGLAYAAVPIYTLFCKVTGFGGTT
                     QRATAASSVILERTVTVRFDANTAPGLGWRFEPLQRTVDIKIGENTLVFYRATNTTDR
                     AIVGSSTFNVAPESAGIYFQKLECFCFKEQLLEPGQTIEMPVSFYVDPRFASDPETKG
                     LSQITLSYTFHKVEKPAAAAMPVKAAGPPGGT"
     gene            23615..24529
                     /locus_tag="PK264_03405"
     CDS             23615..24529
                     /locus_tag="PK264_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HRD74975.1"
                     /translation="MADGHTKHHDYHLVNPSPWPLMGSIAAFVMAIGAIVWMQSLGGK
                     TGLFGMRGPWLFAAGMLLVLFTMFMWWRDVIKEAHAGDHTPVVQLHLRYGMILFIASE
                     VMFFVAWFWAYFDVAFYPGTVETLPNSVYEWGMTERAKLTGSKWPPVPSPDGSFKATF
                     DPWGLPLVNTLILLLSGTTVTWAHHALLENNRQGLIWGLLLTVILGAIFTGLQAYEYG
                     HALFGYSKHIYGATFFMATGFHGAHVIIGTLFLLVCLFRAMKGHFSPKQHFGFEAAAW
                     YWHFVDVVWLFLFLCIYVWGAGQYAGGH"
     gene            24914..25349
                     /locus_tag="PK264_03410"
                     /pseudo
     CDS             24914..25349
                     /locus_tag="PK264_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665276.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
     assembly_gap    25350..25367
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25368..25878
                     /locus_tag="PK264_03415"
     CDS             <25368..25878
                     /locus_tag="PK264_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562156.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRD74976.1"
                     /translation="RPEIARKLKRPLVKVIGVGEANKIQAGGVGLDLTYSAGAAAGKA
                     AFEMAGIAPKDIKYASIYDSFTITVVMQLEDLGFCKKGEGGKFVSDGNLISGVGKLPF
                     NTDGGGLCSNHPGNRGGMTKVIEAVRQARGEAHPKVQVKNHDLVLAHGTGGSLGTRHV
                     GSTVILERE"
     gene            25884..26276
                     /locus_tag="PK264_03420"
     CDS             25884..26276
                     /locus_tag="PK264_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRD74977.1"
                     /translation="MAETEKIYATVPATPGAEAYWEGAKAGKLMVQWCGDCKKPHHYP
                     RGVCPHCMSTKLEWREAKGTGTIYTYSVMHASKPAYVIAYVTLDEGVTMMTNIVDCDP
                     AKVKIGQKVKVVFKPSEGGGKLACFAPA"
     assembly_gap    26458..26602
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26730..28070
                     /locus_tag="PK264_03425"
     CDS             26730..28070
                     /locus_tag="PK264_03425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD74978.1"
                     /translation="MKLMWFHLMPYTELPDDFRQKHPSVWVDIHSSLFDPKRAHLMYN
                     DFMDELEYAAECGFDAICVNEHHSNGYGLMPSPNLIASSLARRTRDTAICVMGNSLAL
                     YNPPTRVAEEFAMIDCISGGRLIAGFPVGTPMDTCYAYGQNPSMLRERYLEAHDLVMK
                     AWQEKETFAFNGRFNQQRYVNIWPRPIQEPHPPVWVPGGGSVETWRWCAEMDYVYCYL
                     SYYGYKAGQATMKGFWDEMARLGKDRNPYRAGFLQFVGVAESRSEAMERYAEAAEYFY
                     GRCLHVDPRFATPPGYNTEATQRVGLESQVRMAAEAAMKAQAEAKPHGGKDALAGGTT
                     GTTAGSRFSMVAKDMADIVDRGYVIVGSPKDVAEQLEHVAKTLNVGHFMMLLQFGNMS
                     KELTKYNTKLFAEQVMPKLRPLFADWEDRWWPKPMAEEKRAIVPAYRPSLKAAE"
     gene            complement(28207..28455)
                     /locus_tag="PK264_03430"
     CDS             complement(28207..28455)
                     /locus_tag="PK264_03430"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRD74979.1"
                     /translation="MSFRLGENLGYAGKTLLAALGHDAMTVADQRLSGVQDEAIYNVA
                     AVERRALITLERDFGEVLRIPPEPTEGIIVLICQGRLS"
     gene            complement(28452..28694)
                     /locus_tag="PK264_03435"
     CDS             complement(28452..28694)
                     /locus_tag="PK264_03435"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRD74980.1"
                     /translation="MCSPLDRITVEPRVCFGKPCIKGTRIWVSLILDMLADGMSDGAI
                     LAEHPQLTEDDIRAAIAYGAEVARERIVPVDFRAAE"
     gene            28882..>29137
                     /locus_tag="PK264_03440"
     CDS             28882..>29137
                     /locus_tag="PK264_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74981.1"
                     /translation="MTQPATTTVEINGQQCRVWSKGKGPKIGFLAGFGGLPRWMPFLD
                     KLAESRTVIVPSLPGHPGALGHTKNDSHLDWVVTVKELVDK"
BASE COUNT         5025 a         9219 c         9304 g         5158 t
ORIGIN      
        1 cgagaccatc atggtcaact gcaacccgga aacggtttcg accgactacg acacgtcgga
       61 ccgcctgtat ttcgagccgc tgaccaccga agacgtgctc gaaatcctga acgtcgagat
      121 gtcgaacggc acgctcgcgg gcgtgatcgt gcagttcggc gggcagacgc cgctgaagct
      181 cgcggagacg ctggccagcg ccggtgtgcc gatcctcggc acctcgcccg atgcgatcga
      241 cctcgccgag gatcgtgatc gcttcaaggc actgatcgac aagctcaaat tgaagcagcc
      301 tgagagcggc atcgcgcgca gccccgaaga ggcgcgcgct gtggctgagc ggatcggcta
      361 tccggtcgtc atccgcccgt cctatgtgct cggcgggcgg gccatggaaa tcgtctcgga
      421 cagcgccgag gtcgagcgct acgtccaacg tctgtcgcag acgctcgacc ggccctccga
      481 actcgtcgtt tcggatcgtc gcccgctgct catcgatcgc tacctctcgg acgccatcga
      541 ggtcgacgtc gactgcctcg ccgaccggca gacgacgttt gttgccggta tcatggagca
      601 tatcgaggag gcgggcatcc attcgggaga cagtgcctgc tcgctgccga cacactcgct
      661 cgacagctcg atcgtcgtcc gcctcgaaaa gcagacgcgc gatctggcac tcgccctcgg
      721 cgtcgtcggc ctcatgaacg tgcaatacgc cgtcaagggc cgcgagatct acatcctcga
      781 ggtgaacccg cgcgcctcgc ggactgtgcc cttcgtcgcc aaagtgatcg gccagccgat
      841 cgcgggcatc gcctcgcgcg tgatggcggg cgagccgctc gcgagcttcg gattgaagcc
      901 gaagcgcctc acgcatgtcg ccgtcaagga ggccgtgttc ccgttcgcgc gcttcccagg
      961 cgtcgatccc gtgctcggtc cggagatgcg ctcgacgggc gaagtcatgg gcatcgaccg
     1021 caactacgcg ctcgccttcg ccaagagcca gctcggctcg ggcaccgtgc tgccgagtgc
     1081 gggtgctgta tttctgtcac tccgtgacag cgataaggat gccgttctcg gtgccatgcg
     1141 cgagctgtcc gacatcggtt tcaggctcgt tgctactcga ggaacgcgcc ggcatctcga
     1201 aagccacggc attccttgcg agctcgtaaa caaagttctc gaaggccggc cgcacattgt
     1261 cgatttaatg aaaagtggtg aaattcaatt ggttatcaat acgaccgaag gtgccaaggc
     1321 tctggcggac tcaaaggaca tccgccgcac ggccttgatt aaccacattc cctattatac
     1381 gactctggca ggtggtctcg ctgcagctga ggccatcaag gccctccggg ccgacattct
     1441 cgatgtggcc ccattgcaaa gctacgtcgg gaaacccacc tgacgaggtc gtcacgacgt
     1501 tgtcggacgt aagggttctt tgccggacac gcgccgcctt cgactatggg cgaggctcag
     1561 ggcaaaggac tggcaaatcc ccaaatcctg ggtgaaactc ggttgaggag ccagcactgg
     1621 cttcttcgtg aaggaggcgt gagccgatgg ccatggaaaa ggtgccgatg accctcgagg
     1681 gcttcaagaa gctcgaggag gaattgcatc gtctgaaagc cgtggagcgc ccgcgcatta
     1741 ttcaagcgat cgccgaggcg cgtgcgctcg gcgacctctc cgagaacgct gaatatcact
     1801 ccgccaagga ggcgcagggc ctcaacgagg cgcgcgttgc cgatctcgag gacaaggtct
     1861 cgcgcgccga ggtcatcgat ccggccaaga tgtccggcga caccgtgaag ttcggcgcga
     1921 cagtgacgct cgaggacgag gatacgggcg agaaggtcaa gtacaagatc gtcggcgatc
     1981 ccgaagccaa cgtgcgtgaa gggcgcatct cgatctcttc gccgatcgcg cgcgcgctca
     2041 tcagcaagtc gaagggtgaa acggctgacg tcaccacgcc caagggctcg cgcagctacg
     2101 agatcctgaa gatcgagtac aagtgacggg actcagaaga ccgggggcag cgccgcaagt
     2161 gtcggcagca cccggtcttt cggcgagagg atcgccctgt cggccgtgat acgccagtcg
     2221 atggcgagcg cgggatcgtc ccagcggagg ccgccttcgt gctcgggggc atagagatcg
     2281 gtgaccttgt agatcacctc ggtgtcggga acgagcgtca catagccgtn nnnnnnnnnn
     2341 nngggcacat agagctgcct ccagttgtcg gcgctgagtt cgacggcgaa gtgctggccg
     2401 taggtgggcg agcctttgcg gatatcgacc gccacatcga gaatcgcccc gcgcactacg
     2461 cgcacgagct tgtcctgagc gcggggcggg cgctggaaat gcagcccgcg caccacacca
     2521 gcctcacgcg agagcgcgtg gttgtcctgc acaaaggtcg cctcgatccc ggctttcgcc
     2581 agcgtggcgg cgctccacgt ctccgagaag aagccgcgct cgtcccggaa gatgcggggc
     2641 gccagcacct tcacatccgg gatgtccagt gttcggacgt ccatcgtgga agcctcctca
     2701 gtgcttcatg tcgctctgcg agggcgggct tagtgtaacg acgttcgttc ccgacagcgg
     2761 ttcgctcgcc ggtgccgagg ccgtggcggc cttccacgcc gcatactcct tctggaactt
     2821 tacgtagccg aacggaatga gggcgaggta gccaagcgta ataatggcga gggtcacgta
     2881 agggtaggtg acaagcaagg cgaccaccag cacgacgccg aagaggatcg gcatcacgag
     2941 ctcgcgcgga atgcgctcgc cgagcagctt tcctgaaaac gtcgggatgg tcgagacgag
     3001 catgaacgcc agcgccagcg tgtagaccgt gatgacggcg ggataggcct tcgggtcgaa
     3061 gcccggtatg ccgaggccct cgagatagag aggcagcagc acggtcaacg cgccggcggg
     3121 cgccggaatc cccgtgaaat agttggactg ccagcgcggc ttgtcgacat cgagcataga
     3181 gttgaagcgc gcgaggcgca gtgccatgcc gcaggcgaac agcagaacgg cgatccagcc
     3241 aaagcgtttg aagtcgccga ggccccacgt gtagacgagg atggccggag cgacgccgaa
     3301 gttcacgaaa tcggcgaggc tgtcgaactc ggcgccgaag cgcgactggg acttgagtgc
     3361 gcgcgcgagg cggccgtcga caccatcgag gagcgcggcg aagacgatga gcgccagcgc
     3421 ccattcgaac tgcttctcga tcgccatgcg catcgcggtg atgccggcgc agaggctcag
     3481 cagcgtgaag aagttcggca ccaacagtcg gacggggatc ttgcggcgcc ggaagaggcg
     3541 acggcggctc ggcgcgccgg tcgggtctcg caggtccgcg gggtcgatag ggtccatcgc
     3601 tcatctcccg gtgacgggct ctgcccgagg ggtcgcgttg gcccctcatc gcaagccagc
     3661 gcatcacttg caactctctt gcagggcgat tgtggttcgc cggcggcgtt attcaggtgc
     3721 agcgcggggc gctgttggcc cctccgcggt tcccgtgtag gaagcgtgca tgaccgatac
     3781 gatcctgatc ctcgacttcg gctcccaggt gacgcagctc atcgcgcggc gagtgcgcga
     3841 ggcgggcgtc tactcggaaa tcgttccctt cagccagggg gaggtggcat tcgaccggct
     3901 ccgccccaag ggcataatcc tctccggcgg gcccgcgagc gtctactgga gcgaggcgcc
     3961 gcgcgtgccg cgccggcttc tcgaggcggg cctgcccgtg ctcggcatct gctacggcaa
     4021 ccagacgatg gtgacggagc tcggcggcaa cgttgagggc ggccacaatg ccgagttcgg
     4081 tcgggctgac atagacatcg tcgaggattg cgcgctcttc aagggtgtgt ggtcgaaggg
     4141 cgagcgcgac cacgtctgga tgagccatgg cgacaagatc acgcgcatcc cggacggctt
     4201 ccgcgtcgtc gcccgttcgg acaatgcccc ctatgccact atcgctgacg aacgtcgccg
     4261 actctacggt gtgctgttcc atccagaggt cgcgcacaca ccgcgcggcg ccgcgctgat
     4321 ccgcaacttc gtgcacaatg tcgccggctg ccgcggcgat tggacgatgg cggccttccg
     4381 cgcgtcagcg atcgagcgca tccgtcgtga tgtgggcaag tcgcgcgtga tctgcggact
     4441 ctcgggtggc gtcgattcgg ccgtcgccgc ggttctcatc cacgaggcga tcggcgatca
     4501 gctcacgtgc atcttcgtcg accacgggct cttgcgcctc aacgagggtt cggaagtcgt
     4561 caagctcttc cgcgagcact acaacatacc gctcgtgcac gtggacgcga gccgacagtt
     4621 cctgaccgcg ctcgatggcg tctccgatcc cgagaggaag cgcaagacca tcggcgcgct
     4681 attcatcgac gttttcgagg ccgaggcgaa gacgatcggc ggagctgagt ttctcgcgca
     4741 agggacgctc tatccggatn nnnnnnnnng tcatcgagag cgtctcgttc acgggcgggc
     4801 cgtcggtcac gatcaagtcg catcacaacg tcggcggttt gccggcacgc atgaacatga
     4861 agctggtcga gccgctgcgc gagctcttca aggacgaggt gcgcgcgctc ggcggcgagc
     4921 tcggcttgcc cccggcattc atcggacgcc atccattccc ggggccgggc cttgcgatcc
     4981 gcatcccggg ggcgatcacg ccggccaagc tcgatatcct ccggaaggcc gacgcgatct
     5041 ggctcgacga gatcaggaag gccggcctct acgacacgat ctggcaggcc ttcgccgtcc
     5101 tgcttcccgt gcagaccgtc ggcgtgatgg gcgacgcgcg cacgtacgag gccgtctgcg
     5161 cgctgcgtgc cgtcacctcg acggacggaa tgacggcgga ctacttcccg ttcccgcacg
     5221 atgtactcgg gcggaccgcg acgcgcatca tcaacgaggt gcgcggcatc aatcgcgtgg
     5281 tctacgacat cacctccaaa ccgcccggca cgatcgagtg ggagtaggta gcaatgttgc
     5341 tacaatattt tgcacccctg gaccgtcgat accataattt gagcacttcg aaggtgtcga
     5401 catgaccatc acgacgattt cgagccgcaa gttcaatcag gatgccggcg gcgctaagag
     5461 agccgctgcg aatggccccg tcatcatcac ggatcgcggg cggccggctc atgtgctgct
     5521 caactacgcc gaatatttgc gcctcacccg acaagggcct agcctgctgg acgtcgtggc
     5581 aatgcctggg ggcgagcaca ttgagttcga ggcaccgaaa ctaaaggggc cgttgtccaa
     5641 gtctgtcgac ctcgactgat gttcttgctg gatacgaacg tcgtctccga gttgcgcaat
     5701 atgtcgcgcg cgaacccgtc ggttcgtgct ttggcagcaa gaacgccagc ccaactactg
     5761 tggatgtcga cgatttcggt cctggaactt gagatcggcg tcctgcgcgc cgagcgccgt
     5821 gacccgcccc aaggtgttct gctccgcaga tggctggagg ggcaggtcct tgtccagttt
     5881 gaagagcgat tgctgatggt cgatgctgtt gtcgctcgcg cgtgcgccgg gatgcacgtg
     5941 ccggatcctc gacctgagcg agatgctctg attgcagcga cggcgaaggc gtttgggttg
     6001 acaatcgtca cacgcgatac ggccgacttc gagcccatgg gtgttccggt tctcgatcca
     6061 tggtcgaccg agcagtcgta agccgagcac tgctgaagac tccacgagtc gcgaaacgtg
     6121 caactcagga cggaggcagc tcacaattgg gcattgtgca cccaggactt ggtcgcgtcg
     6181 ttggcgagag gccaattgct ccctgcgcca gaagtagccg gcgccgatgt cgtgcctgct
     6241 tattgatctc gatcaaggag aagggcgctc ttccgtctgc atcctgaggc ttcgagggcg
     6301 aagcttccac acgacatggg cgaggcagtt atggcaagga caccgacgtc agaaggtccg
     6361 gcagccgctc ccggatcggt ttcgcttgtg ccacctgtcc agccaacacc cagcacgaag
     6421 cctcctgcca cgaatggcag cgcgctctcg gcagacggtc cctcgctcat gccgcttgcc
     6481 gccgaccccg acaacaagac ctcgatcgcc cagcgccgcc gcgatcccga ctacatccgc
     6541 cgcgcctttg agagcggaga gtacccctat cagacgcggc tacgcacggc cgagtacgag
     6601 gccgccatgg ccgagctgca ggtcgagctc ctcaaggcac agaagtgggt gaaggagtcg
     6661 ggcgagcgca tcgtcatcct gttcgaaggg cgggacgcgg caggcaaggg cggcacgatc
     6721 aagcgcttca tggagcatct caatccgcgc ggcgcgcgcg tcgtcgcact cgagaagccg
     6781 accgaccggg agcgctcgca gtggtatttc cagcgctaca tcaatcacct gccggcaggc
     6841 ggggagatcg tcttcttcga ccgctcctgg tacaatcgcg ccggtgtcga gcgcgtcatg
     6901 ggtttctgca acccgaacga ttacctcgag ttcatgcgac agtgcccgga tctcgagcgg
     6961 atgttcgtgc gctcggggat ccgtctcttc aagtactggt tctccgtgac gcgggcggag
     7021 cagctccgcc gcttcaaggc ccgcgagacc gacccgctga agcagtggaa gctctcgccg
     7081 atcgatcttt catccctcga caagtgggac gactacaccg aggccaagga ggccatgttc
     7141 ttctatacgg acactgcgga tgcgccctgg accatcatca agtcggatga caagaagcgc
     7201 gcgcgattga atgccatgca gcacttcctg gcgatcctcg actacccggc gaagaatcag
     7261 gccttgatca gaggacccga cccgctgatc gtcggctcct cggcgcatgt catcgggcgt
     7321 gacgagcata tcctcggaaa gaccttgcat cccgagaagc ggcgccggca cacgtgacgt
     7381 cgttcgccgg gctcgagttc aggttcgttc actcaacatg ccgacggctc gaagaatctc
     7441 gtcgacggcc cgtgtcggcg ccagccgacc cgattgcacg tcggcttcga gcgccggaat
     7501 gcgcgccgcg atccgcggat cagccgcgac ggccgccagg atgcgatcgg cgagcatctc
     7561 ccgcatccag gtgaccgctt gcgagcgccg gcgcgtctcg aaggtgccgg tcccctccat
     7621 cttcgcgcga tgctgctcga gcgtctgcca gagcttgtcg agccccttgt cgtcgcgccc
     7681 cgaaatggtg agcaccggcg gcagccaaag cgatcccgcg ggtgtcaaga tattgagcgc
     7741 cgccatgtac tcggccgccg ctcgagtcgc gcgatcgacg ttgtcgccgt cggccttgtt
     7801 gatggcgagc acgtcggcga gttcgatgat gccctttttg atgccctgca ggtcatcgcc
     7861 gccgccggcg agcaggagca cgacgaacac gtcgaccatc gccgcgaccg ccacttccga
     7921 ctggccgacg ccgaccgtct cgacgatcac gacatcgaaa ccggccgcct cgaggacggc
     7981 catggtttcg cgcgtcttcc gcgtaacgcc gccgagcgta ccggaggatg gcgaggggcg
     8041 gataaaggcc ccgccttgct gcgaaagccg cgacatgcgc gtcttgtcgc cgaggatcga
     8101 gccgccgctc cgggtcgacg tcggatcgac cgccagcacc gcgacccgtc tgcccgccgc
     8161 gatcaggttc atgccgagtt ggtcgatggt ggtcgacttg ccgacgcccg gcacacccgt
     8221 aatgccgatg cggtgcgcgc gccccgtatc ggcgatcagc tcctggagga gtgcggacgc
     8281 cgtcgcctgg tgatccgctc gccggctttc gatgagcgtg atcgcccgcc cgatcgcggc
     8341 gcggtcggcg gcgcgaatgt ggtcggcaag ctcgcggacg agtggggcat cagatcgctt
     8401 caaggtggtc gactctcagt ggcgctggat gcctctttaa ggcacgtagc gcgctgaaca
     8461 aaggagagca atcgccgggc aaatgattga cacgggcgca ttgcacaaag tacggtccgc
     8521 cccgaattcg gcttggtccc accgctcgtg ctaggtggag gggcgtgccg tggctcgaag
     8581 cgcggcaccg tcggtgcccg attacggaaa agaccatgaa agtccgcaac tcgctgaaat
     8641 cgctgcgcgg tcgccaccgc gacaatcgtg tcgtgcgccg caagggccgc gtctacgtga
     8701 tcaataagac caatcgccgc ttcaaggcac gccagggcta gtctcccggc gcaagtgcac
     8761 ctttcgaggt cgtgagccgg gaccatgtgc ccggctcgtt tcgttgcgtt tgccactcgc
     8821 aatttccagt ggaccactgc catgagccag aaatcgccgc cactgccacg cgtcttctcc
     8881 ggcatgcagc cgacgggaga gagcccgcac ctcggcaact atctcggcgc catcgtcaac
     8941 tgggtcgaga tgcagaagac gcacgagtgc atctattgcg tcgtcgacat gcacgctatc
     9001 acacagtggc aggatcctgc cgggctcaag gattcgatcc gcgccgtggc cgccgcctac
     9061 atggcagcag gcctcgatcc cacgcgctgc attctcttta accaatcgca ggtcggcgag
     9121 cacgccgagc tcgcctggat attcaactgc gtcgcgcgca tcggctggct caaccgcatg
     9181 acgcagttca aggacaaggc cggcaaggat cgcgaggcgg cctcagtcgg gctctacgcc
     9241 tatccgaacc tcatggcggc ggacattctc gtctatcggg cgacgcatgt gcctgtcggc
     9301 gaggaccaga agcagcacct cgagctctcg cgtgacattg cccagaagtt caacaacgac
     9361 ttcgccgacg ggatcgcccg gcttggctac acgggcgggt tcttcccctt gcccgagccg
     9421 atgatcctcg gaccggcggc gcgcgtgatg agcctgcgcg acggcaccaa gaagatgtcg
     9481 aagtccgatc catcggacct ctcgcgcatc aacctgagcg acgacgccga tacgatcgcc
     9541 cgcaagctgc agaaggccaa gaccgatccg gcggcgcttc cgagcgagga ggctgggctc
     9601 gacaaccggc ccgaggcgga caatctcgtc gggatctacg cggggcttgc ggcgaagtcc
     9661 aagtccgatg tgctgcgcga ccacggcggc gcgcagttct cgaccttcaa gaaggccctg
     9721 atcgaactcg cggtcgcccg catcgggcca atcagcgcgg agatcgcgcg gcacaccgcc
     9781 gaccccgccg agatcgaccg ggtgctgcgc gatggcgcag accgggcgcg cgccattgcc
     9841 cgcccgatca tggacgacgt caaggacatc gtcggcttcc tgcgctgagg cgagggcttc
     9901 accctgcggg tgaactgcga agggccttca gcccttcgcg catcccgatc agggcctggg
     9961 ccctgaaccc gatccaaagt atgggtccaa gggctaaccc ttggcgggag ctcgagggca
    10021 gcgcccccga actgctgccc cgaacggcac acgcgttagc aggatcgtaa ccatcttccg
    10081 ccacgctcac gtcaacgagt accgttgcgt cagcgtaggt gtgaaatgtc cctgtcgtgt
    10141 tcgggcccgg tcgcggcccg cagttgcctt cgtgtctcga aattcctcgc tgtcacggca
    10201 atcggctgcc tggcagggct tgtcgttgcc ggatgtgctt cgaagagcac gacgagctac
    10261 gatgcacgtc cgccccagca tgttgccgcg gccaagccgg ccgagatgga ggacgacggg
    10321 cgcgaagccc agcccgcggc cccgcgtcac ctgaagtcac aaccggatga tccgcgcgag
    10381 ccttggagcc cgaactacgg cggcccgagc ccgagaccgc tcacgccggc cgtcccgccc
    10441 ccggcccgcg cggcgatgat ccccgacgac ctgccgccgg attttcgcaa gcgattggtc
    10501 acgcgcgcag actgaggttc agcggttggc tcagctcacg gcccgccacg tcccatcagg
    10561 attgcggcag gccgtgcccc gcatcgtctg catgcgcccc tcgatgtaga cggtgtgcgc
    10621 atagtcgcgg cagtgatgta cgccgcgccg atagggcgcg cctggtacaa cctcgccgta
    10681 gcggccattg tcgggattgc gccagccgac cggcgtcccc gaggtgccgc gctcgagcgc
    10741 gcgcaattcg gcgtcttgcg cgagcagacg gtcctgccgg tccatggagc ggccgatctc
    10801 gctcccgatc acgccgccga tgaaggcgcc cgtcgccgtg gcaacgatgc gaccggcgcc
    10861 tttgccgacc tggttgccga gaatgccgcc ggcgaccgcc ccgaccacga gacccgtgtc
    10921 ctgcttgctg ccgtcgccgg cgcatccggc ggcaagtgtg ccgacgatgg gcaggatcag
    10981 cgcgatgcgc cagcggcgga tgctgcgatg gggctgtgtc acggcgacct cacggaaggt
    11041 gtggcggttg agcgctgcga tcgaagcggg ttgagcgctg gtctgcttcc tgttgaggct
    11101 agggagtgat tcgggcgtga atgtgctggg cggccgatac ggctagagag gctgcgtcct
    11161 gttgccgatc cgcctcaggt ggccggcagc tcgacgatga cccgaagacc tccgagtggg
    11221 ctctcatcga gctggaacgc acccctgtag gtctcgatca ggtcggagac gatggtgagg
    11281 ccgaggcccg aaccgggttt cgtctcgtcg aggcggcgcc cgcgtttgac accctgcgcc
    11341 ctctgctcgg cggtgaggcc cttgccgtcg tcatcgaccg tcagcacgag acgcttcgcg
    11401 ccctcggccg agcgcacctg catgtcgaga gtgatcctgg atctcgccca cttgcaggcg
    11461 ttgtcgagga gattgccgat cagctcctcg agatcctgtt tctcgccctg gaagcgagcg
    11521 ctcgcgtcgc cggtcgcgac gatgtcgagc gccttgtcgt cgtgaagctg ctcgagcacg
    11581 gcgatgatgc tgtcggccac gggcttcacg tcggtcgagc gcacgaccac gccaacgagc
    11641 gccgcgatgc gggcccggtc gagatagcgg ttcacctggt cgcgcatgac ggccgcctgc
    11701 tcgctcacct tgatggcgaa cggcgtcttg ctttcgttgg cctcgttgat gatcacggcg
    11761 agcggcgtct tcagggcgtg cgcgagattg ccgacctggc ggcgcgcgcg ctcgatgatc
    11821 tcctggttcg agacgatgag cgcgttcacc tgctcgcgga gcggttcgac ctcggctggg
    11881 agcagcgctt cgagcctctg cgcatcacct gaacggatgg cgccgaggcc gtcctcgatc
    11941 tggcggagcg gccggaggat gaagcggaac gaggcgaaca tgagcaccgc cagcgcaagg
    12001 ccgacgaaac cgaaggccca gacgagctcg cgcctgaagc gggcgatgtc ggcatgcgtc
    12061 tgatcgagcc gccccgcgat cgcataggag taccagatgt ccttgtgctg cggcgaggga
    12121 ttgaggatcg cctcgatgat gcggatcggc tcgctgccgg gacccttggc gtcggccagg
    12181 cgcacgaacg tgccggtgaa ttgatcgttg aagcgtggca cgcccgggac cagcgggatc
    12241 tcgacgccgg ggatcgactg atagctcgtc gtcttggtga cgagtggcgc gccgagctcg
    12301 ctcggcagcc tgatcggctt gccggcgagg gactccgaga ggatgcgttt gccgccggcc
    12361 ccatccttgg gcgtgatctg ccagtaccag ccgctgtcgc ggtactggaa attgtcgtcg
    12421 ttggcgaagc gtgccggaac cgggatgccg gacaggttct cgctcgagag atgcatttgc
    12481 agcagcagct cgaacagctt gtcgtcgaag ctgtcgagca cctcctgctt atggcggcgg
    12541 tcgatgagga aacccgccag cggcaggacg atgatcgtcc aggcgagacc gctcagcagc
    12601 aacttcacgt agagtgagtt aagcttcatc gccatcctga gcgaggcggt agccgagccc
    12661 gcgcaccgtc ttgatgaggt cgcccgggat cttgcggcgg aggcggccga tgaacacctc
    12721 gatcgtgttg ctgtcgcggt cgaagtcctg ctcgtagagg tgctccacaa gttcggtccg
    12781 cgagacgacg cggccgttgt ggtggaggag gtaggcgagc agtcggtact cgtgcgaggt
    12841 gagcttcacg agcgtgccgt cgtaggtgac gcgcgcggtc ttggtatcga ggcggagcgc
    12901 gccgcactcg atcacgggct tggcgtggcc ggccgcgcgc cgtacgcggg ctcgcacccg
    12961 ggcgagcagc tcctccatat ggaagggctt gccgacatag tcgtcggcgc cggcattgat
    13021 gccgctcgcc ttgtcggaga agcgatcacg ggccgagaga atgatcaccg gcatgttgcg
    13081 atcggcacgc cgccactgct cgagcacgct gatgccgtcc atcttgggca ggccgatgtc
    13141 caggatgacg atgtcgtagg gctccgtctc gcctaggaag aagccttcct cgccgtcgct
    13201 cgccgtgtcg acggcgaagc cttccgccgt cagcgcggtg acgagctgtc ggttgagatc
    13261 gggatcatcc tcgacgacga gcgcgcgcaa gactttctct ccttcggccg gcggaccggc
    13321 accgagcaat ctgggagccg ccctgccgcg cggttgcggc agggaaaact ctgtccatca
    13381 aatatagcgc ataagtcgct ccgccccaat gccttgcatc cactcgggta gcctgtgatt
    13441 tgccgggagg cacggaaggg ccgaaactgc cagtgcaggc gattcgctcg gagtcaggag
    13501 catgcatcag caacccatcc cgggccttat cggtctcgat catgtcggcc tcactgttcc
    13561 cgacgtcgac agggcgacgg ccttcttcgt ggacgtactc ggcgccgagg cgctctacga
    13621 tatcggaccc ttcacggatc cggcggagtg gatggcgagc catcttgcgc ggcgcgcgga
    13681 tgcccgcatc gagcgcatgc gcgtcctcgc cgttgccaac ggtccgatgc tcgagctgtt
    13741 cgagctttcg ggggcagcac tcgagacagc gggcgtgcac cttgcattct atgtcgagga
    13801 catggaagcg gcgctcggcg cggtgaaggc gcatggagcc gtcatccaga gcggtccagt
    13861 cgagatgacg gaagggccga gcgcggggct gacctggctc tacttcacag ccccatgggg
    13921 cctgcaactc gagctcgtca gctatccccg cggtatcgct gcgctgagag agcgcaagac
    13981 gccggtatgg cggccacgcc gttgagtgat caggcggccc gagccaccgc gtcgatgagc
    14041 cgggcaagct cgatatcgcg gctggtgagc ccgccggcat cgtgcgtggc aagcgttacc
    14101 ttcacgcggt tgtagacgtt cgaccactcg ggatggtggt cgaggcgctc ggcatgtagc
    14161 gccacccgcg tcatgaagcc gaaggccgcg ttgaagtcgg cgaaacgaaa ctcgcggctg
    14221 attgcctcgc gcccaggcgc cagctgccag tcggacaggc ttgtcagcgc cgcgctccgc
    14281 tctgcctcgt tcagtcgctc tgccatggtg acctcctcgt gagctcgccg gcgccgtcgc
    14341 cctgcctcag caacttcccg agattgccca tctcccggcc cgagacggcc ttgttgtcaa
    14401 gggacgacag ttggtgagca acctcgggaa cggccgcatg gcgaactatc ggacactgct
    14461 cgaacgactg ctcttggcgg cctgcctcgt gtcggccccg agcttcggcc gtgccgaagg
    14521 aggcgacgag gcaggacctg tcgcgctccg gaacatcgga gccccgcgtg cgctcgccaa
    14581 caagctgccg cgctccgagg ccgatcaggc gctccttcgc ggttggccgc tctatcggac
    14641 cgagcgtgga caggaggcgt tcaacgcggc gatggccacc ctgaaggcga ccgaaggcgc
    14701 tccgccggcg gcctcgacct tcaggggctg cgtggggctt gcatgtcgtc ttgcgcttcc
    14761 tcgcattggc atgaacggct ggctgcccgc cggccgcatc tgggtatcgc cgaccgaata
    14821 tgtgctaatc gcacacagct ctcggtttcg cgatggtcag cggatgcgcc gccgcatggc
    14881 acgtgagatg cgctacctcg tgctccacga gttccacaac ggttcacgca acaccgatgt
    14941 ctacgacacg atctcctcgc acagccgcgc ggtgttcgtg ccgctctaca tgagccggca
    15001 aggcatcgat gcgcatgggc acgcatttgt cgtcgtcgtg caggtggcgc cctacgacgt
    15061 cgcgagcatc cacgcctcga atttcgggaa tgccgggccg ggcatcgagg ttgccaagaa
    15121 ctatggtgat ccccttgatc cggtccagtc gctcgccggc attctcgttg cgacgatgat
    15181 caaggaggcc gctccccagc tgaaggtcgt gcatcatcgc ggcagcgaag gccgtgccat
    15241 gctcgaggcc tatgacaacc gcgtggcccg tgtggcgagc cgggctggct tagcaccggt
    15301 tgcgttgccg ttcgtcccgg cgccgcccgc gcgccttgcc tcggtatcgg ccgggacggt
    15361 tgcgagcctt gtaaggcgcg ggacaggctc ggtggtcgtg ccggtcgccg accgcggcat
    15421 cctgtacggc cggacacgtg tcgctggcat tgccccacgc ccaatgctga tcgagccggt
    15481 cgcacccgtg acggccgacg acctcctgct gccgccacgc ttgatcggtc ctattctgcc
    15541 ggccgtcagg ccctcggtga tcggtcacgg tgctccagtc aattgattgc cgccgcgcgc
    15601 ccggcgtgct gtctatgtga caccacgcgt cgcgtgtacc cgggaggttc tagtggccaa
    15661 gctcaccgcc gacgccaagg gcgtctacgt catcgccgca acgcccttca aggaggacgg
    15721 cgcgatcgac cacgccagca tcgagacgct caccgagttc tatatccgct cgggcgtcac
    15781 gggcatgacg atcctcggcg tcatgggcga gttccagaag ctctcggaga gcgagacgct
    15841 cgacgtcgca cgccgcttta tcagctgcgc aaaggggcgc gtcccggtca tcgtcggcgt
    15901 atcaaacccc gggacgacgc agctcctcaa agtctcgcgc gccgccatgg agctgggggc
    15961 tgccggcgtc atggtgatgc cgacgccggg cctcaggacg gacgaggccg tctcggccta
    16021 catggggagc gtgctcaccg gcctcggcgc ccaaattccg gtctgcgtgc aggactatcc
    16081 gcagctgacc ggcgtctact tctcggccga ctgcttcctg cgcctggtcg atgcctatcc
    16141 gcagatcgtc atgttcaagc acgaggagag ccctggcctt cggaagcttt cggctgtgcg
    16201 ccgtgcgact gacggaaaga cgcgacgccg gatttctatc ctgtgcggca acggcggcat
    16261 ccacctgccg caggagtatg cgcgcggcgc cgatggcgct atgaccgggt tcgcctatcc
    16321 cgacatgatg gcgcgcatgc atgcgatatt cgaggcgggc aagcgcggcg aggccgagga
    16381 cctctacgat ctctacctgc cgatcctgcg ccacgagctg caaccgggca tcgggctcgc
    16441 actgcgcaag gagatcctgc ggaggcgtgg cgccatcgcg tccgcggccg tgcgtgcccc
    16501 tggcccgaag ctcgatgcgg acgacatcgc cgaacttgac gcgctacttg cccgcctcga
    16561 agccaagctg cccatagaac cataggttga gaacgggtgt cgtgacccga aaggcacgca
    16621 ccgattgcat agtgccggtt taaacacgtt taggtggcaa tttttgttgc ggctggcggt
    16681 gcggaaaaat tagaacgctc ctaccgatat tcctgttctt gagtccatgc gggtgccgcc
    16741 ctgagtgcga tgtgcagcgc ggagtgggcc tcgaggacgt tctgggacag ccgaccccct
    16801 ctgttctgcg tgtgtgatcg tgtgctgcgt ggccggaatt cccccggcgc gtgctgatct
    16861 cgatgcccaa gccgaacgag gataagatgg ccactcagcc gtacctgcga ttgatttgct
    16921 cgactgccgc tgccttggcc ctcggcggct gcatggtgca tccggtggca cttaccgagg
    16981 atgagatcgg cgcacgcgcc cgcgtcaacc tggcaacact gcagacaggc gatgccccgg
    17041 taagcggcgc gatcgacctc tatgaggcca tggcgcgggc cctgaaattc aacctggatg
    17101 cgagggtcga agccgcgcag acagcattgc gcctcgccga actggacctc tcacactgga
    17161 gcctgttgcc gaacgcagtt gccaactcag gctatgcagg tcgcaacaat tacaatgcct
    17221 cgagctcgtt caacgtcatc accaatacgc cgaatgcctt cgtgtcgacc tcgacgtcgc
    17281 aggagaagcg catcctgtcc ggcgacatcg gcttcagctg gaatgttctc gacttcggcc
    17341 tctcctacat ccgggcgcgc cagtcggcgg acaaggtgct gatcgcgggc gagctgcggc
    17401 gcaagatcat cctgcggctc atggaggacg tgcgcacggc ctacttccgt gcggtctctg
    17461 cagagcggat gatgcgcaag ctcatggtgc tggatgcgcg ggcgcgcgtg gctcttgccg
    17521 gctcacggac ggtctacaag gaccggcaga catcgccgat cacggcattg acctacgagc
    17581 gtgagctcgt cgagatccga cgtaccatac aggagctggc gcgggatctg tcggtagcaa
    17641 aggctcaact cgcggcgctg atgaacctga agcccgggac ccagttcgcg ctcgtcgttc
    17701 nnnnnnnnnn nnnngccgca tcccccgcgc tcggcctcgg cgccgccgaa atgatcgcca
    17761 tcgcccagcg caaccggccg gaactcaagg agatcgccta ccagcggcgc atcaacgacc
    17821 aggaaatgca cgcggccctg ctagagctgc tgccgagctt caatacctat gtggcgacga
    17881 acttcgacag caacgctttc ctgctcaacc agcattgggc gagctggggc gccaaggcat
    17941 catggaacct cttgaaggtc ttccagtatc ccgccaagcg ggaggtggtc gagcagcagg
    18001 atgaggtact ccggcagcgt gagcttgcga tcaccatggc ggtgataacc caggtccacg
    18061 tgagccgcgc ccgctacctg ctcgctcgcc gcgaactcgg cattgccgcc gagtatctca
    18121 gtgtgcagac ccgtctcgtc ggccagatgc gcacagaagc gaaagccgac cggatcagcg
    18181 agcagacgct catccgcgag gagatgaaca cgctcgtcgc cgagatccgc cacgacatca
    18241 cctacgccac tctgcaaagc gcctatgcca cgctcttcgc ctccataggc ctcgacccct
    18301 atgccgaagc ctcgacgagc gatgttcctc tgaaggagct ggcagcacag cttcgctcac
    18361 tctggttcga gcggggccag ttccgcaagc gtgcatggga tgcagtcgcn nnnnnnnnnn
    18421 aagtgagatg ctggccggtc tttcagctcg cgggattgct cccgcgagcc gctaggcgtt
    18481 agtggattgc ctgcggctgg ttcccccttt gcgcggccgg attgagcctg tatgccgcac
    18541 tcgcgccact tgagttgcct catggcatga tgtttgatgc gcgctcggat tgggcccgtg
    18601 gtgtgttttc cgcgtcccct ggggcatggc tcgaaaagtc atcaagcatg actcagaaag
    18661 tcatcaggca tggctcaaga aggtcatgcc ggggcacgta gcggcgagaa cgttcgtggg
    18721 ttgccctatc atgggcatga gagaggaagg gcgtggcgag cgggccgcgc caggcaggag
    18781 caagcgatga ttgagcgcgc cagatcggtg cttgggcgag tcgcggccca cgtcagtgcg
    18841 agtgtgacgc tcgcgctgtt tgcgatgata acgggggtca cgggggccgc cgcacagaag
    18901 ttaccggatg gcatggggca gcccttcccc tggcaggttg gcctgcagaa gcccgccaca
    18961 gaggtcgcgc gcgagatcaa tatcttccat gaccgggtga cgatcctgat cacggtcatc
    19021 accatcttcg tgcttgtcct gatgctctac gtgatgttcg ccttccgtga gggtcgcaat
    19081 ccaagcccgt cacgcacgac gcacaacacc tttctcgaac tcgcctggac gattgtgccg
    19141 gtcatcatcc tgatcgtgat cgcggtaccg tccttcaagc tcctttacct ccaatactcg
    19201 tttccgaagc ccgacctcac gatcaaggcc atcggcaacg cctggtactg ggagcatgag
    19261 tatcccgacc acggcaactt caaggtcacc tcgaacatgg tgaccgacga ggaggtcatc
    19321 aagacgaaga tcggcgcgga cgagttctcg aagcgctaca agggccttgc cggcatcgag
    19381 cgcgacaagc ggctctatgc cgacgcgcag ccgatctggg ccgagcgcaa ggagccgcgc
    19441 ctgctcacgg tcaacaatga gatcgtcgtg ccgctcggca aggtggtgca cctgctcgtg
    19501 acggccaacg acgtgatcca caactggacc attccatcat tcggctcaaa ggtcgacgcg
    19561 gttccagggc gcgtcacggc gacctggttc cggcccgaag tcgctggcct gttctacggc
    19621 cagtgctccg agctctgcgg caaggaccac gccttcatgc cgatcgccgt gcgcgtcgtt
    19681 cccgatgccg tgttcgcgca gtgggccgat gccatgaagg cgcgcgaccg gcgccgtgca
    19741 cgcgagatcc tccaacgcgc ggaagccgag gcgaacggca ccacggtcgc ggcggtatcg
    19801 ccatcctcga gcgggaaata gtgagccgcg gtcctggcgc tccgggcacc gggacgcgcc
    19861 ggcaacttct gaagtttgac tttcgatggg ttggaaggca cgtcattgca ttgccggcaa
    19921 gagcaatgtg agtgtccaac cgacaaagac aagacgatcg atcacgatca agcgagacgg
    19981 gcaacgttca tggcgagcaa ggctcacgca gaccacggtg acgggcacga ccacgcgccc
    20041 tacggcatca cacggtggct ctattcgacg aaccacaaag atatcggcac gatgtacctc
    20101 atctttgcga tgatggccgg cgtgatcggt ggcttcctgt cggtgatgat gcggctcgag
    20161 ctgcaggagc cgggcctgca gtacttctcc aacacgcacc tcttcaacgt gttcgtgacg
    20221 gggcacggcc tcatcatggt gttcttcatg gtgatgccgg ccatgatcgg cggcttcggc
    20281 aactggttcg tgccgctgat gatcggcgcg ccggatatgg ccttcccgcg catgaacaac
    20341 atctccttct ggctgcttcc ggcttcgttc gcgctgctgc tcctgtcgat gttcgtgccg
    20401 ggtgccggca cctgcgagat cggcaaggac atgtcgggct gcggtgtcgg tgcgggctgg
    20461 acggtctatg cgccgctttc gacgaggacc ggccactccg ggccggcgat ggactttgcg
    20521 atcctctcgc tgcacatcgc cggtgcctcg tcgatcctcg gcgccatcaa cttcatcacg
    20581 accatcttca acatgcgcgc gcccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20761 nnctaccagc acttgttctg gttcttcggc catcccgagg tctacatcct gatcctgccc
    20821 ggcttcggca tgatcagcca catcgtctcg acgttctcgc gcaagcccgt gttcggctac
    20881 ctcggcatgg cctatgccat ggtcgccatc ggcgtcgtcg gtttcgtggt gtgggcgcac
    20941 cacatgtaca cctcgggcat gagtgtgaac acgcaggcct atttcgtgtt cgcgacgatg
    21001 gtgatcgcgg tgccgacagg cgtgaagatc ttctcttgga tcgccacaat gtggggcggc
    21061 tcgatccgct tcacgacgcc gatgctctgg gcgcttggct tcatcttcct gttcacagtc
    21121 gggggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnng gcacacttcc actacgtgct gtcgctcggc gccgtgttcg ccatctttgc
    21241 aggctggtac tactggttcc cgaagatgag cggctacatg tactcagaga ccttgggcaa
    21301 ggcgcacttc tggatcacct tcgtcggcgc caacgtgctg ttcttcccgc agcacttcct
    21361 gggtcttgcc ggcatgccgc gacgctacgt cgactatccg gaagcacttg ccggttggaa
    21421 caaggtgtcc tcgttcggtt cctacatgac ggctttcggc acgatcgtgt tcttcatcgg
    21481 cgtgttcctc gcgctttcgc gcaaggagaa ggcacaagcc aatccgtggg gcgagggagc
    21541 gaccacactc gagtggacct tgccgtcgcc gccggccttc cattccttcg agaagcttcc
    21601 gaggatcacc gcaggtgatc atcactgagc ttgctcccgg gctgccgcat ggcggcccgg
    21661 ggccctgttg aactctgtcc cgattagggg cggttcgcgg tgccgagagg gcacgcgctt
    21721 aagatgaggc cggagccggt atggcgacgc ttgagaacga gcaatcgcgg gaacccagcg
    21781 ccttcgaggg ggcgatgggc ggcgaggtcg gcgactacat cgcgctgatg aagccgcgcg
    21841 tgatgtcgct ggtcgtgttc acagcgctcg tcggccttgt cgtcgctcct gggtcgcttc
    21901 acccggcgtt ggcgctgatt gccatcgtgt gcattgccgt tggcgctggc gcctcgggcg
    21961 cactcaacat gtggtacgat gccgatatcg atgctcgcat ggcgcggacg gccgggcgcc
    22021 ccattccggc tgggcgcatc tcgcccgacg aggcgctgac cttcgggatc gtgctttcgc
    22081 ttgcctccgt agtgacgctg ggcgtgctcg tgaattgggt cgcaggcatc ctgctggcat
    22141 tgacgatcgt ctactacacg gtcgtctaca ctatgtggct gaagcggcgc acaccacaga
    22201 acatcgtcat cggcggcgct gccggcgcct ttcctccgat gatcggctgg gctgccgtct
    22261 ccggcggcgt ctcgctcgag agcatcgtcc tgttcctcat catcttcatg tggacgccgc
    22321 cgcacttctg ggcactcgcc ctgttccggt cgggcgacta tgagcgcgcc ggcgtgccga
    22381 tgctgccggt ggtctcgggt gccgcggaga cgcgccgcca gatcgtcatc tactcggtcc
    22441 tgctggtacc gctcgcggtg gtgcccagct tcatcggcct gggcgggctt gcctacggtg
    22501 tcgtctcggt cggacttggt gggtggtttc ttgccctcgc gttgcagctc tatcgtgcag
    22561 gtgactgccc cggcagcctg acgatcgcaa agaagctttt tggcttctcg atcctttatc
    22621 tgttcgcgct cttcgccgtg ctgctcgtcg aggcgcttgt cgcccggggg ggcgcatgag
    22681 cactgtggcg cgtgatggcg atcgggatgg cctgcgccgg cagcgctcgc gctcgatcgc
    22741 cattgcactg tcgctggtgg cgctcgtcat cgtgttctat gtcgcgacgc tgatccacct
    22801 cgggccgaac gcgctcaata agagcgccta caatcggccc gagtccggca aggtcaagat
    22861 cgttaccgac cccacgggga agagcgaatc tttcaagggc gtggaccccg actgcaagaa
    22921 ggcgggcaca tgctgatgtc gcgtgacatg agggtcgcgg cgatctgcgc cagcatcgct
    22981 ttcggcatgg tgggtctcgc ctacgccgcc gtgccgatct acacgttgtt ctgcaaggtg
    23041 acgggcttcg gcggcacgac gcagcgcgcg acagctgcct cctccgtcat ccttgagcgc
    23101 acagtgacgg tccgcttcga cgccaatacg gcccctggtc tcggctggcg cttcgagcct
    23161 ttgcaacgca ctgtcgacat caagatcggc gagaacacgc tggtgttcta ccgcgcgacc
    23221 aacacgaccg atcgcgcgat cgtcggctcg tcgaccttca atgtggcgcc agagtccgcc
    23281 ggcatctatt tccagaagct cgagtgcttc tgtttcaagg agcagctgct cgagccgggc
    23341 cagacgatcg agatgcccgt gagtttctac gtggaccccc gctttgcaag cgatcccgaa
    23401 actaaagggc tctcgcagat cacgctttcg tacacgttcc acaaggtgga gaaacctgcg
    23461 gcagccgcga tgccggtgaa ggcagcaggg ccgcccggcg gcacatgaga acttttgggc
    23521 cgcacccgcg cgagcggagc acccagacag tatgagtgat gaggcgcatc gcgccacgcg
    23581 aacggactga gatcgggaag ggacggagct ggacatggca gacgggcaca cgaagcatca
    23641 cgactatcat ctcgtcaatc cgagcccgtg gccgctcatg gggtcgattg cggcgttcgt
    23701 gatggcaatc ggcgccatcg tgtggatgca gtcgctcggc ggcaagaccg ggctcttcgg
    23761 catgcgcggg ccctggctct tcgcggccgg tatgttgctc gtgctcttca cgatgttcat
    23821 gtggtggcgt gacgtcatca aggaggcgca tgccggcgat catacccccg tcgtgcagct
    23881 ccacctgcgc tacggcatga tcctgttcat tgcctcggag gtgatgttct tcgtcgcctg
    23941 gttctgggcc tacttcgacg tcgccttcta cccgggcacc gtcgagacgc tgcccaactc
    24001 cgtctacgag tggggcatga cggagcgtgc caaactcacc ggctccaagt ggccgccagt
    24061 gccgtcgccg gacggcagct tcaaggcgac cttcgatccc tggggcctgc cgctcgtcaa
    24121 cacgctgatc ctgctgctgt cgggcaccac ggtcacctgg gcgcatcacg cgctgctcga
    24181 gaacaaccgc cagggtctga tctggggcct gcttctcacc gtgatcctcg gcgcgatctt
    24241 cactgggctg caggcctacg aatatgggca cgcgctgttc ggctactcga agcacattta
    24301 cggcgcgaca ttcttcatgg cgacgggctt ccacggcgca cacgtgatta tcggcacgct
    24361 gttcctgctt gtctgcctgt tccgcgcgat gaagggccac ttctcgccga agcagcactt
    24421 cggcttcgag gcggcggcct ggtactggca cttcgtcgac gtcgtgtggc tgttcctgtt
    24481 cctgtgcatc tacgtgtggg gcgcaggcca atacgccggc gggcactgat cgcactgcgc
    24541 actcccgatc cctgatggcg cccggccgaa aggtcgcggc gccattatcg tgagcaagcg
    24601 ggcctttgcc gggctgggat gcgcgcctgc atgcggctcc ccctcgacgc ctcgcccgat
    24661 ccgtcgcaat ctatcgccac aagttcaggc agggcaggag cgcatgagta tcaaaggcaa
    24721 agcctacatc gtcggggcct acgagcatcc gacgcgaaaa gcccccgaca agtcgctggc
    24781 gcagctgcat gccgaggtcg ccaagggcgc gatcgaggac gcggggctca ccaaggacga
    24841 catcgacggc tacttctgcg cggccggcga tacgcccggc ctcggcggcc tctcgatgat
    24901 cgagtacatg gggctgaagc tccgccacat cgacacgacc gatacgggcg gctcgtccta
    24961 caacgtgcat gtcggccacg cggtcgaggc gatcgcggcc gggcgctgca acgtcgcgct
    25021 gatcacgctc gccggccgtc cgcggtcgga aggcatggcg accggcacgg cgccgcgact
    25081 cggcaatccg cagcagcccg atctccagtt cgagttcccc tatggaccgg cgactgccaa
    25141 catgtacgcc atgtgcgcca tgcgccacat gcaccagttc ggcacgacgt cggagcagct
    25201 cgcgtgggtg aaggtcgcgg cctcccatca cgcccagcac aacccgcacg ccatgctgcc
    25261 aaaggtcgtg accgtcgagg acgtggtgaa ctcgccatac gtcgcgagcc cgctgcaccg
    25321 gctcgactgt tgcgtcatct cggacggcgn nnnnnnnnnn nnnnnnngcg gccggagatc
    25381 gccaggaagc tcaagcgccc gctcgtcaag gtgatcggtg tcggcgaggc gaacaagatc
    25441 caggcggggg gcgtcggcct cgatctcacc tattcggcgg gtgcagccgc cggcaaggcc
    25501 gccttcgaga tggccggcat cgcgccgaaa gacatcaagt acgcttcgat ctacgacagc
    25561 ttcaccatca ccgtggtgat gcaactcgag gacctcggct tctgcaagaa gggcgaaggc
    25621 ggcaagttcg tctcggacgg caacctcatc tcaggcgtcg gcaagctgcc gttcaatacg
    25681 gacggcggcg gcctctgctc gaaccatccc ggcaaccgcg gcggcatgac gaaagtcatc
    25741 gaggcggtgc gccaggcgcg tggggaggcg catccgaaag tccaggtgaa gaaccacgat
    25801 ctggtgctgg cgcatggtac gggcggctcg ctcggcacgc gtcacgtcgg ctcgacagtc
    25861 atcctcgagc gggagtaaga cccatggccg agaccgaaaa gatctacgca accgtccccg
    25921 cgacgccggg agccgaagct tattgggaag gcgccaaggc cggcaagctg atggtgcagt
    25981 ggtgtggtga ctgcaagaag ccgcatcact acccgcgcgg cgtgtgtccg cactgcatgt
    26041 cgacgaagct cgaatggcgc gaggccaagg gtaccggcac gatctacacc tacagcgtca
    26101 tgcacgcctc gaagcccgcc tacgtgatcg cctacgtcac gctcgacgaa ggcgtcacga
    26161 tgatgaccaa catcgtcgat tgcgatcccg ccaaggtgaa gatcggccag aaggtgaagg
    26221 tcgtcttcaa gccgagcgaa ggcggcggca agctcgcctg ctttgcgccg gcgtgaggcg
    26281 cgatcgggtc tgtgcctgag gcctcgtcgt gacgcgagtt gcctcggtgc cagccagccc
    26341 ccggcatcct agcgaaggcg aggacccagg tctccgagcg agggcacggc ggcggccaat
    26401 ccgcggtgca ctgggtcctg ccctccggcg ggatgacggt ggtgggctgg ctggccgnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nncgacatgg caatgcaaaa aatgcgcgct cggccgcctg
    26641 ccatatcact tgtcagacgc ccggctttgc cgcagatttt cgcgtgagtg tgccggacgc
    26701 cacggtgccg accccatgga ggagcgccca tgaagctgat gtggttccac ctgatgccgt
    26761 atacggagct gccggacgac ttccggcaga agcatccgtc cgtgtgggtg gacatccatt
    26821 cctcgctctt cgatccgaag cgcgcgcacc tcatgtataa tgatttcatg gacgagctcg
    26881 agtatgccgc cgagtgcggc ttcgacgcga tctgcgtgaa cgagcaccac tcgaacggct
    26941 acggcctgat gccgtcgccg aacctcatcg cctcctcgct cgcgcggcgc acgcgcgata
    27001 cggcgatctg cgtgatgggc aactcgctcg ccctctacaa cccgccgacg cgcgtcgccg
    27061 aagaattcgc gatgatcgac tgcatctcgg gcgggcgcct catcgcgggc ttccccgtcg
    27121 gcacgccgat ggacacctgc tacgcctacg ggcagaaccc ttcgatgctg cgcgagcgct
    27181 atctcgaggc gcacgatctc gtgatgaagg cctggcagga gaaggagacg tttgccttca
    27241 acggccgctt caaccagcag cgttacgtga acatctggcc gcggcccatc caggagccgc
    27301 atccccccgt ctgggtgccg ggcggcggct cggtcgagac gtggcgctgg tgcgccgaga
    27361 tggactacgt ctactgctac ctctcctact acggctacaa ggccgggcag gcgacgatga
    27421 agggcttctg ggacgagatg gctcgcctcg gcaaggaccg caatccgtat cgtgcgggct
    27481 tcctgcagtt cgtcggcgtc gccgagagcc gctcggaggc catggaacgt tatgcggagg
    27541 cggccgaata cttctatggc cgctgcctgc acgtcgatcc gcgctttgcg acgccgcccg
    27601 gctacaacac ggaggcgacg cagcgcgtcg gcctcgagag ccaggtgcgc atggcggcgg
    27661 aggccgcgat gaaggcgcaa gccgaggcga agccacacgg cggaaaggac gcgctcgccg
    27721 gcggaacgac agggaccacg gccggatcgc gcttctcgat ggtggcgaag gacatggccg
    27781 acatcgtcga ccgcggctac gtgatcgtcg gctcgccgaa ggatgtcgcg gagcagctcg
    27841 agcacgtcgc caagacgctg aacgtcggcc atttcatgat gctgctgcag ttcgggaaca
    27901 tgtcgaagga gctgacgaag tacaacacca agctcttcgc cgagcaggtg atgccgaagt
    27961 tgcgcccgct gttcgccgat tgggaggatc ggtggtggcc gaagccgatg gcggaggaga
    28021 agcgggcgat cgttccggcc taccggccga gcctcaaggc ggcggagtga ggtctcaggc
    28081 gtcgtcgggc ttggttcgtg actggtggat acggaccagg cccggttcga cgatccacag
    28141 tcgtcctgcg agcggctccg agggcaggat ggcggccaaa tctgccattc gcgcttcgat
    28201 tttggcttac gacaggcggc cctggcagat gagaacgatg atgccctcgg tcggctcagg
    28261 aggaattcgc aggacctctc cgaagtcacg ttcgagggtg atcagcgcac gccgctcaac
    28321 ggcagcgacg ttatagattg cctcgtcttg cacacccgag agccgttgat cggcgacggt
    28381 catggcgtcg tggccaagag ctgcgagcaa agtcttcccg gcataaccga ggttctcacc
    28441 aagcctgaaa ctcattccgc ggctcggaag tcgactggaa caatgcgctc tctggccact
    28501 tcggccccgt aggcgatggc tgcgcgaatg tcatcttcgg tgagttgagg atgctccgcc
    28561 aggatagcgc cgtcagacat tccgtcagcg agcatgtcga ggatgagtga tacccagatg
    28621 cgggtcccct tgatgcaagg tttgccaaaa cagacacgtg gttccacggt tatgcggtcg
    28681 agtggactgc acatgctggc tatcacaatc cggaaaggtt cgtgcgcgag tacaacctgc
    28741 actgaattcg cggcgaagat cgttggcacc gcactggacc tgcgccgtca cgccttgtca
    28801 gctgactatc catatcacaa tgtgaactcg tgcggcagcg ccgcaggacg tgatcaggag
    28861 cattggaatg cgagtacgac cttgacccaa cccgccacga ccaccgtcga gatcaatggc
    28921 cagcagtgcc gtgtctggag caaaggcaag ggaccgaaga tcggctttct cgcgggcttc
    28981 ggcggcctgc cgcgctggat gccgttcctc gacaagcttg ccgaaagccg cacggtgatc
    29041 gtgccgtcgc tgccggggca cccgggcgcg ctcgggcata ccaagaacga cagccacctc
    29101 gattgggtgg tgactgtgaa ggagctcgtc gacaagg
//
LOCUS       DAORNH010000018        28616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1251_length_28616_cov_2.585519, whole genome shotgun sequence.
ACCESSION   DAORNH010000018 DAORNH010000000
VERSION     DAORNH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28616
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1251_length_28616_cov_2.585519"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PK264_03445"
     CDS             <1..256
                     /locus_tag="PK264_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRD74982.1"
                     /translation="ADARTPVAELEARLGRKLLDGEEADEIDTLGGLVFSIVGRVPAR
                     GELLRHRSGVEFEVLDADPRRVKKLKIHLPRPLDAPAAIG"
     gene            287..913
                     /locus_tag="PK264_03450"
     CDS             287..913
                     /locus_tag="PK264_03450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD74983.1"
                     /translation="MSSKELGNVYAAKSVAEVARYYDDWSATYDTFMSGVGYRHPAIC
                     MSLLTRWLPRGEGPILDAGAGTGLVGEWLGIVGYPHVEALDISDGMIAIARKKSVYKA
                     FHMAALGGPLPFPDGAFAGIVSSGVFTTGHVGAEGLDELIRICRPDGVIVLTVKDTLW
                     NGGFAEALRGHVRHRRIRLVDETPPYSSMPGEVGNIPSRAIAVKVGSD"
     gene            1023..2285
                     /locus_tag="PK264_03455"
     CDS             1023..2285
                     /locus_tag="PK264_03455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD74984.1"
                     /translation="MAAAEMKRAIVIGAGIVGACTGLELERRGYEVTLVDRLPPGEGC
                     SFGNAGILSSQAVVPMATPGIHKKALGMLTDPDGPLTVRLSSLRLTVPWLWRMLKASR
                     AERMAPIADAMKALYATTVELHEALAREAGVPELVVPGRYLYVYRDPANADVEKGLGW
                     RLRRERGAELEVFDGAALQEIEPELSPIYKRGVRVGPLAKTTNPHRLTAAYARLLERK
                     GGRILKAEVREIVPAATATAPIAVETSAGRLEAELLVVAAGAWSLDLLRPLGVKFSLI
                     AERGYHLTYPEPGFALSHMVSEGERYFALTTMEMGLRFAGTDELSHADDAPRWRRADV
                     LARFAPEMFPRLGTANPSRWSGPRPGTPDSLPVIDRVPGQPRILIACGHGHLGLTGGP
                     MTGRIVGALASGERLNLDLAPYAVTRFQ"
     gene            2385..3326
                     /locus_tag="PK264_03460"
     CDS             2385..3326
                     /locus_tag="PK264_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRD74985.1"
                     /translation="MSELPGSVAATRALLERGGYLADEALSTTVFLGLKLARPLLLEG
                     EPGTGKTEIARVLATVLERPLIRLQCYEGLDIASAVYEWNYARQLLHIRASEGAGRGG
                     DAGAIERDIYTDAYLVKRPLLQALEFSKSGRAPVLLIDELDRADEPFEAFLLELLADY
                     QVTIPELGTIRAEAPPITILTSNRTREVHDAVRRRCFYHWVDFPSAERERAILALRVP
                     EAPRELTRQIVAFVQGLRQMDLYKTPGIAESINWAQALTSLDCVALDPTAVDTTLGTL
                     LKYQDDIARIQGSEAARLVEKVRGDAREVARAAGAGQ"
     gene            3529..3990
                     /gene="rplM"
                     /locus_tag="PK264_03465"
     CDS             3529..3990
                     /gene="rplM"
                     /locus_tag="PK264_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRD74986.1"
                     /translation="MKTYNAKPGEVEKKWLLIDAKGLVVGRLATIIATRLKGKHKPSY
                     TPHVDMGDNVIVINADKVVFTGKKREDKVYYRHTGYPGGLKELTARQIFEGKHPTRVI
                     EKAVERMLRRGPQHRQLMRNLRIYAGTEHPHDAQQPTTIDVAKLNRKNVRA"
     gene            3996..4493
                     /gene="rpsI"
                     /locus_tag="PK264_03470"
     CDS             3996..4493
                     /gene="rpsI"
                     /locus_tag="PK264_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRD74987.1"
                     /translation="MATETKSLQDLGALTGAAPPPGADTPSAPVYQKKVDAKGRAYAT
                     GRRKNATARVWVKPGKGVITVNAKDYTKYFARPVLQMLLRQPLNAAQRLDQIDVTATV
                     RGGGLSGQAGAVRHGISRALTHIEPELRSVLKKGGFLTRDSRMVERKKYGKMKARRSF
                     QFSKR"
     gene            4665..5072
                     /locus_tag="PK264_03475"
     CDS             4665..5072
                     /locus_tag="PK264_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74988.1"
                     /translation="MARLAADPVAVRAWAKAIAGNAVAELSDGDRSFLATLAEFDGTP
                     PMSVRQREYLDGLIERTERYPVVDGFRADRLVRLIHEARLDLDEDEEARIADLAAGGA
                     GLALSPRQWRWVLAVSRRLGLIDDPYIALPGRG"
     gene            5164..5406
                     /locus_tag="PK264_03480"
     CDS             5164..5406
                     /locus_tag="PK264_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74989.1"
                     /translation="MILAATTHEIASTDVASANQARSKTSIVGYIAALATELRRAVSA
                     NAAYEDLNRRSDAALSDLGLGRSTIAREIHTTHYID"
     gene            complement(5514..5978)
                     /locus_tag="PK264_03485"
     CDS             complement(5514..5978)
                     /locus_tag="PK264_03485"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD74990.1"
                     /translation="MLPTGKPAPKFDLPTDDGGRVSPATLKGAPAVIYFYPKDDTSGC
                     TREAIDFTELAAEFKAAGAVVVGISPDSCASHAKFRKKHNLGVVLAADEDKAAAEAFG
                     VWVEKSMYGRKYMGVERSTFLIDKAGKLVRSWTKVKVPGHAAEVLEAVKSLK"
     gene            6526..10104
                     /locus_tag="PK264_03490"
     CDS             6526..10104
                     /locus_tag="PK264_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRD74991.1"
                     /translation="MRFVTGAMKLFVVVAVLFAFGAGLAYVRLLHSPISARMLIGPIE
                     RAIGAGLADLTVRIEDVELATGDGNLLELRVKNMRLVGPDGKAAAEVPRAAVELSTAS
                     LLLLDVQPRRLDLIEPSFNIAYRPGKGMSLSLNPGPLDAPASGSVGGPASGAPAATAT
                     DPAGESEATAAARGVIEMLRGARRQSSGGGLEQVGVRNASVILDNNGIRSVWRILGAE
                     LSLQHHQKRSVLVGEMRMASDRSPWTIGIRAEESDKTKLLSVELAIANVVPEEIGRHW
                     PGLGVLRAIELPMSGKATIEIDARGEIIEGTLSIGSVEGSVSGARPPVIDQIKLAASY
                     ASSSGRVSISPSTLASGASRITFVGEIAPRPPTADAPASWSFVIAANDGVIAADTSVP
                     TLPVERLAIRGTLTPESNLVRIDEMQLQAGKASVAMAGDIWFKGERVGATLEARSAPM
                     SVATFRALWPAVIAPKSRKWVAGHVGKAQIESFVLKTEPSSDTRPGAKPLKAMITAEI
                     SGVEVAALKWLPMLDIPRLLIRIDGPQLDMTAPEGRLMTGPGRIAQIRALKVTSQDLD
                     VEVSQAELILKGNASVASALDLIERHPASGFNRASVGLDSADGRVEGQARFKFPMVED
                     IDAADVRIEGSARVSDGRVRNLVGKHDVQSAAITFEFTDKAIDAKGQFIVAGVPARLG
                     WKHIIGVPPDRQPEMNITATLDNGDRTQLGIDTAPFLTGETKLDVSVLRSGREAPTVH
                     VNVDLTQSELAIDGLGWKKPVGIAARMDFDVARGRQNRIELQNLKVTGGDIAIEGWVG
                     LGPDNKPREFHFPEFSVQIVSQLEVRGTLKPDNVWDLKVTGARFDGRNVFKSMLAAGR
                     TSERPAPPAKDRAGLDMTAEIGAVLGFNENALKGLKLRLSRRGEHLTALDMSGTLSSG
                     EQLLAQLQRDGQRQRLLRAQTSDGGNAFKLIGFYPNMIGGLMQLEVDLDGRGAADKTG
                     TLWVRNFLVLGDQIVSDFVATTEDGNQPREAPKRPRERVVRQQIEFELLRVPFSIGHG
                     QFVLHSAQIRGPLIGATLRGKLDFMARQLALNGTYIPLSGINRALSGIPILGDILTPK
                     GEGVFGITFAIEGSMERPVLTANPMALIAPGIFREIFQMGPEDTRITPRETPAPKARI
                     EPRGRASSVPPTSGPPPASAPSPDSVSGWTSGTETKPRSAPASPKK"
     gene            10122..10961
                     /locus_tag="PK264_03495"
     CDS             10122..10961
                     /locus_tag="PK264_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HRD74992.1"
                     /translation="MAGSSIDHLTPRSLAEGAAAIVATADLAAKIALAKLTAEAWRDN
                     RLSLSRRTGMPMPDRPGRPPRPVLLPPRRMPKRSTEGEKGRFALMHALAHIELNAVDM
                     TWDLIGRFHAESVPAAFFDDWVRVGYEEAAHFEMVTQRLGELGGSYGDLPAHDGLWQA
                     AQATGHSLIARVAVVPLILEARGLDVSPALIASLTAAGDKASAAVIEVIYRDEKTHVA
                     CGSRWFRFLCEREGRPLEATFHRLVRENFRGPVKPPFNDAARSEAGLTPGFYKPLARL
                     IGD"
     gene            11245..11751
                     /locus_tag="PK264_03500"
     CDS             11245..11751
                     /locus_tag="PK264_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRD74993.1"
                     /translation="MFTKKPDRDTPPPIDLTKSAPPIPPPITSAPRPASSRPSTTSSV
                     IGPDLTVMGNIVSKGGVQIDGEIQGDLHAMNVVVGENARITGGIIADEVIVRGTVMGS
                     IRGRSVSLQSSSKVEGDVYHQSLAIEQGAYFEGKSRRSEDPLASTTRPDGPLTNGAAS
                     TSSSTLPS"
     gene            11789..13153
                     /locus_tag="PK264_03505"
     CDS             11789..13153
                     /locus_tag="PK264_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74994.1"
                     /translation="MIGPRVVFALLVVMIAVAGCGRAAPDAGEARLCRSVIPALEVIS
                     ARPQRERVRIIDERRIAGHAAVEITYRYVSEPPRIGRRITCTFERRVAAAEAQPRDLI
                     EVRLEQRPMSAIRLHLLKRFWLEANASAAIDPEPIRGAARAPEIGPGLAFTLQQTLLT
                     VPGIVNTALLAGAFALVYGLSNRINLAFGVMAIVGGYGALLGLLSTVGLAGATPAGLI
                     VALAFAVWSASVTSAGTAAGLLVPLQRERSQIMLIAGLGLAIALEEFMRLIQGARVQW
                     VPPIFNQPFALARADDFVVAATALSLLVIAVAALAIAVLVLLMRRSRFGRRWRAISDD
                     ELAAGMVGIDARRTAVATYALAGALAGVAGFLSTLLYGGVGASGGMLLALKALLAAVI
                     GGIGSVGGAILGGMLLGVIEGLWSALLPIEHRDAAIFTLLVAALILFPGGLFDRRARE
                     VRSA"
     gene            complement(13207..13635)
                     /locus_tag="PK264_03510"
     CDS             complement(13207..13635)
                     /locus_tag="PK264_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74995.1"
                     /translation="MTTDPNVTATAPAPATPPRKPALRTPRNMRWLNFLVAAIAAVLI
                     ATMWRQFFSHSGFDMKNAWVLGMLTAVGLSSLVEAIFPDRIHLFISSWLINASGAAFL
                     VSYAAGGERWLGLVGLGLMLLSFLIALAMTARARHVELEG"
     gene            complement(13728..14342)
                     /gene="nuoE"
                     /locus_tag="PK264_03515"
     CDS             complement(13728..14342)
                     /gene="nuoE"
                     /locus_tag="PK264_03515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HRD74996.1"
                     /translation="MAVRRLDPTQPKSFAFTPENEAWARAKIAQYPDGRQASAVIPLL
                     WRAQEQHAGWLPEPAIRLVAEMLGMAYIRVYEIATFYTMFQLSPVGRKAHVQVCGTTP
                     CMLSGSEALIEVCKRRINGEAGKLSADGDFSWEEVECLGSCANAPMVQIWKDTYEDLT
                     PELFERVLDGFAKGSPPKPGSQTGRVSSCPAGGPTTLTALAKTK"
     gene            complement(14361..14816)
                     /locus_tag="PK264_03520"
     CDS             complement(14361..14816)
                     /locus_tag="PK264_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD74997.1"
                     /translation="MSVDLILWLAVAAFAWGLSLVTYRWFALINGWPMGDWQANRPGL
                     PFALGVIAMTMALVFGLARGGGTGLIVPVLGALLALAWTAIFRVGAQASLLLAPLATL
                     ALVLSWISTPLPFDPRPRAATSDDRLPARRYDDITTVVRPQPGPTKRGP"
     gene            15390..16346
                     /locus_tag="PK264_03525"
     CDS             15390..16346
                     /locus_tag="PK264_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD74998.1"
                     /translation="MINGSKIGIALGGGAARGWAHIGVLRALVQAGIKPDIIAGTSIG
                     AVVGGCFAAGRLDELEGFARELTRRKVFGFLDFNLAGSGLITGQKLVDRLEAYLGDLD
                     IEDLPAKFVSVATEIGTGHEVWLGSGKLVAAMRASYALPGIFKPASVGGRWLFDGALV
                     NPVPVSVCRALGARYVIAVNLNSDLGGKGTVIQAFDTSEDTATAQPAEPEGRGARRLI
                     ARQLLGPSSDAPGISTVVVDAFNIVQDRIARSRLAGDPPDAMISPKLASIGLFEFHRA
                     DEAIQQGVMAAAREVEDLGRELALRRRIAEKPLPATIPAYQS"
     gene            complement(16362..17159)
                     /locus_tag="PK264_03530"
     CDS             complement(16362..17159)
                     /locus_tag="PK264_03530"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD74999.1"
                     /translation="MQLTDKQIAQYDRDGFLHFPEFFAATEVAAMRREVQRVSRIESE
                     MVVREGSERSPKAMFRMHESDGPTASPVFHDVVRLPRTLGLARQLLRDDKLYLHHTKV
                     NIKAAIEGSVWPWHQDYGSWANDGIVRPDLLTIMVGLDPATEMNGCLYFLPGSHVHGR
                     LDPYFDTSTVYKVWSVTPADMKRMIAEHGEPVAITGKAGSLTVFHCNLMHASGHNLSA
                     NDRWQAYFCYNTCANRPRDVEKPRADWVRSRNWTPLEFVADSAVAMA"
     gene            complement(17185..17613)
                     /locus_tag="PK264_03535"
     CDS             complement(17185..17613)
                     /locus_tag="PK264_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75000.1"
                     /translation="MFGKLLKSVQSALRQDSSQSRWFVMLNHNPDDASVPDDVTLFGS
                     LEALAGDLSPEDLARGGYEAVCSRGAVVTLRPARDGESVEATIAAVPSAEGRAAAFLR
                     HYLVDASETRPTLGLDPAAIEREGDLARLAAMVPPKDIGA"
     gene            complement(17645..18622)
                     /locus_tag="PK264_03540"
     CDS             complement(17645..18622)
                     /locus_tag="PK264_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD75001.1"
                     /translation="MLTPHRQPKQALSSYKEPPMTPEQILSIKPKILTQKQRERYFED
                     GHIMLERVIPADWVERLRAVTQKMVDESRAITKRDAKWDIEPDHTPENPRLRRLSSPN
                     DHHPTYWEYASSEKSVLPDIMADLVGPDVKFHHSKLNFKWAKGGAEVKWHQDIPAWPH
                     TNYSPATAGTYLYDCNREQGPMGFIAGSHNGPIYSEYDDKGNWTGCLAKEDVAKLDLK
                     KVVWLEAPAGSISVHNCRSLHYSEPNNSPIARPLLLNVFSNANAMPYSVNPIPSRYSG
                     AIVRGNPARWAFHDPRPCLLPPDWSGGYSSIFAKQQGEDKGAPAMGGMM"
     gene            complement(19444..20379)
                     /locus_tag="PK264_03545"
     CDS             complement(19444..20379)
                     /locus_tag="PK264_03545"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD75002.1"
                     /translation="MTPEQVLSLKPKVLSQKQRERYFRDGYLLLEKFLPDSWVERLKA
                     ATEAMVEKSRGVTKSDAVWDLDKGHTREAPRLRRLSSMNDHHPTYWDYASAADSPLPD
                     AIADLVGPDVKFHHSKLNFKWSKGGDEVKWHQDISFWPHTNYSPCTAGTYIYDCNAEQ
                     GPLGVLVGSHQGPVVDQYDKDGKWVGCMSAEDAGRIDMSKVVYLEGPAGSVTIHNCRT
                     LHYSKANWSETPRPLLLNVFSAANAMPYTYNPLQSRYYGAIVRGKPATWAYHDPRPCL
                     LPPDWSGGYTSIFALQQKEDWSKSPPQAAMGGGMM"
     gene            complement(20588..21898)
                     /gene="hslU"
                     /locus_tag="PK264_03550"
     CDS             complement(20588..21898)
                     /gene="hslU"
                     /locus_tag="PK264_03550"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HRD75003.1"
                     /translation="MTNFSPREIVSELDRYIVGQLPAKRAVAIALRNRWRRQQLADDL
                     REEVLPKNILMIGPTGCGKTEISRRLARLANAPFIKVEATKFTEVGYVGRDVDSIIRD
                     LVEVGIGLVRELKRKEVRAQAEKQVEERILDVFVADTQGAARESFRKKLRAGALDDQD
                     IEIKVADSSPQMPTLDIPGMPGSQVGMIQIGEMLGKAFGGRQKSRRMKVREAQAALIG
                     EESDKLIDQEQTVAEAVRSVENNGIVFLDEIDKICSRSDGGRVGADVSREGVQRDLLP
                     LIEGTTVSTKYGPVKSDHILFIASGAFHVAKPADLLPELQGRLPIRVELTSLTREDFR
                     RILTEPEASLIKQYVALMGTEGVTLVFTADAIDALADIAAEVNTTVENIGARRLHTIM
                     ERVLDDISFNAADRSGDTITIDAAYVKRHIAELAKNADLSKFIL"
     gene            complement(21924..22505)
                     /locus_tag="PK264_03555"
     CDS             complement(21924..22505)
                     /locus_tag="PK264_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75004.1"
                     /translation="MSLRPLAGVAVVTSVAASAALAADPEPFDRILPKDSAVCLERHY
                     DAKHLRAHPRQNVIHMRLYRTGEDIARDNGANPPDSYRSFTIAATFRQHPNVVFLESA
                     FCNLDRKTGTTSCGGCGAQPITLSIDGAGRVTLKGYTRAFEIECDEVSKRGTLTGSDD
                     AMIVLASAPAAQCNEAELKKRAPWAVWKKQKAG"
     gene            complement(22502..23665)
                     /locus_tag="PK264_03560"
     CDS             complement(22502..23665)
                     /locus_tag="PK264_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75005.1"
                     /translation="MLTRRHLDDAVAQRIVTTEQAEQLWRLAVGTGAPAKVPLSKAWA
                     PAADRTEPGEERFRIANGFNEIFVALGVILLGIGMLVSLGVDTVNRNFANAITNLGTT
                     AAWAASYMAILWVLAEVLTARSRMLAPSIVIVIALAILAPIAIVPLIDHLTRGGVTLV
                     RYFNQANVSMPPVLLLLPGATFAVAGAHYARFRFPFSLLILALAAVAVIIGTLAAYQP
                     QFFWSAAAKTINWDNVNRLILALGIAIFVVALVFDLTDPERTTNRSDCGFWLHMVAAP
                     MIVHALMSIFAWKSTGGVVPVFVTFGVLALIAIIVDRRAILVAALSYLITAIGVAASQ
                     AGFTGAQIGYLVPIAVGTGIVALGVGWQLVRRGIWTVIPPLGPIDRLRPRRVA"
     gene            complement(23748..>24443)
                     /locus_tag="PK264_03565"
     CDS             complement(23748..>24443)
                     /locus_tag="PK264_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75006.1"
                     /translation="IQAMEGMGFEVLHLYGTTESYGPSTFCAHNSEWDSTIPIAERYA
                     LMARQGIPLVAIDDMMVADPATLAPVPKDGQTIGEIMLRGNTLMKGYLGNEAATEEAF
                     AGGYYHSGDLAAWHPDSYIDVKDRSKDIIISGGENISSLEVEEVLYRHPEIMEAAVVA
                     RPDEKWGESPCAFVTLKPGARPTTAEAVIAYCRANMAGYKIPRTIVFCELPKTSTGKI
                     QKFVLRERARDLK"
     assembly_gap    24444..24485
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24486..25376)
                     /locus_tag="PK264_03570"
     CDS             complement(<24486..25376)
                     /locus_tag="PK264_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75007.1"
                     /translation="MAGTEHPELRKRPANFVPLSPVSFLERAASVFADRIAVAHGARR
                     YTYREFFARARRLAHSLTKAGIKAGDTVAILAPNVPAMLEAHYAVPMIGAVLNPINIR
                     LDPAAIAFCLTHGGAKLFLADREFHATIAPALELLGDKRPIVIDIADEETAGANSFGG
                     VEYEGFVASSDPAFDYPGIADEWESICLLYTSGTTGNPKGAVYSHRGAYLAALSNALT
                     FKLDHESRYLWTLPMFHCSGWTFTWAVTASGGTHVCLRRVEPKRIFELCAEHRVTHMC
                     GAPIVLNMLVHAPESVKRPLA"
     gene            complement(25387..>25974)
                     /locus_tag="PK264_03575"
     CDS             complement(25387..>25974)
                     /locus_tag="PK264_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD75008.1"
                     /translation="GTATRIYWTFKVMGHDKVSILDGGFIGWASAIDAQTKKPVNPLA
                     TDDVKPVAKTFKPAFRAEMIVTAEEVKSAAAAGTPMVDNRPYDFYVGLNKSPAAKRAG
                     TIPGAVSLPEGWLTENGGGRFRTRDQLLKLYRAQSISPTAAHITFCNTGHWASLGWFV
                     SSELLGNKSVRMYDGSMAEWTHDASRPVDVKVKLD"
     assembly_gap    25975..25988
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25989..26337)
                     /locus_tag="PK264_03580"
     CDS             complement(<25989..26337)
                     /locus_tag="PK264_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD75009.1"
                     /translation="MLTRRVAQAMIAGTFAVFAAGLTSAAPSMTPLVDAAFLRANLGK
                     PGLVVLEARGLPKAAYLVGHIPGAVFTDYGKDGWREKSAAGVDGMLPPPEKVAKLIGS
                     LGIDNDSHVIIVPD"
     assembly_gap    26349..26377
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26542..26787
                     /locus_tag="PK264_03585"
     CDS             26542..26787
                     /locus_tag="PK264_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75010.1"
                     /translation="MQALNLADSDDESLRALDFILQGWDAGSEAGIAPEFMAYAALYA
                     GLTDLVGTYGEDAVVKLVTGLVTRVKEGEFTLVRTRQ"
     gene            complement(26792..26989)
                     /locus_tag="PK264_03590"
     CDS             complement(26792..26989)
                     /locus_tag="PK264_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75011.1"
                     /translation="MRIGSPLLDSYAQNVRRFAWAQSAERQMSPGTQVSTGWVTNPYD
                     FNLKQSSPLGNGLPAPASIPR"
     gene            27111..>27439
                     /locus_tag="PK264_03595"
     CDS             27111..>27439
                     /locus_tag="PK264_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75012.1"
                     /translation="MAGTNLDGGGTHQPGRRLGLLRLLILPLVISLAVIAAQALRKPI
                     KAPFADEFYYLTIARDLSLHGVITDGPFKARLPDLLLPAYVTADERPEASRPGRVFGP
                     AYPILVSS"
     assembly_gap    27440..27449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27450..28593
                     /locus_tag="PK264_03600"
     CDS             <27450..28593
                     /locus_tag="PK264_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75013.1"
                     /translation="SFFDPRLRESVRCHVKAFGGSQAHCPNAFATLIAANIAIATVSG
                     LAIFLIGWSLVRSEALAWGMLAIALASGELGFYARSYLSETLTIAAMLAFLLFAVHAM
                     RDFRRHDFALAGASLAMAALTRPAYLYLIYGLAALLLILAVAGRRFVPRITVVHALVF
                     GGAAILVLSPWLVRNLVQFGDPAISKGYAEIILTQRLSYNRMSFAEWGVAWIYWLPDF
                     GDGLARKLFPEALYAKLGWDMPTSYFYEGGSGVFLDKVMAEAGSKELLLGHLVRTYLV
                     GDALKHALVTLPLTMRGLGVAKYLSVIGLPLVWLVGRRMHARGLLLPYLSLALPPLGM
                     ALFHGFVSVNIERYNAPIIALYAFNTAFVVLALIETRRHRGGIAQA"
BASE COUNT         4700 a         9483 c         9417 g         4921 t
ORIGIN      
        1 tgcggacgcc cgcacgcccg tcgccgagct cgaggcgcgc ctcggccgca agctcctcga
       61 tggcgaggag gccgacgaga tcgatacgct cggagggctt gtgttctcca tcgtcggtcg
      121 agtgccggcg cgcggcgaac tgttgcggca ccgcagcggc gtggagttcg aggtgctgga
      181 cgccgatccg cgccgcgtga agaagctcaa gatccacctg ccgcgcccgc tcgacgctcc
      241 ggcggcgatc ggctgagccc gcgatcacaa gccaaagaga gagttcatga gcagcaagga
      301 gctcggtaac gtctatgccg ccaagagcgt tgcggaggtg gcccgctact acgacgattg
      361 gtcggcgaca tacgatacct tcatgtccgg cgtcggctat cgccatcccg caatctgcat
      421 gtcgctgctg acccgctggc tgcctcgagg cgagggcccg atcctcgatg cgggcgccgg
      481 cacgggactg gtcggtgaat ggctcggcat cgtcggttat ccgcacgtcg aggcgctcga
      541 catctcggac ggcatgattg cgattgcgcg gaaaaagtcc gtctacaagg ccttccacat
      601 ggcggcactt ggcggcccgc tcccgttccc cgacggcgcg tttgccggca tcgtgtcgtc
      661 gggtgtcttc acgacaggcc acgtcggggc agagggcctc gatgagctca tccgcatctg
      721 ccgcccggac ggcgtcatcg tgctgaccgt caaggacacg ctttggaatg gcggcttcgc
      781 cgaggctttg cgcggccacg tgcgccatcg ccgcatcagg ctcgtcgacg agacgccgcc
      841 ctattcctcg atgccgggcg aggtcggaaa catcccgagc cgcgccattg ccgtgaaggt
      901 cgggtcggat tgagtgacga acgaggggtc gacgagcggc atgcctgccc cgcctcggtg
      961 ccgctgccaa gccggcgcca ctcggctatg ctcgcgttga aattgcggac ggggacctgg
     1021 acatggctgc agcagagatg aagcgcgcga tcgtcatcgg cgccggcatc gttggcgcct
     1081 gcacgggcct cgagctcgag cggcgcggct atgaggtgac gctcgtcgac cgattgccgc
     1141 ccggggaagg ctgctccttc ggcaatgccg gcatcctctc ctcgcaggcc gtggtgccga
     1201 tggcgactcc cggcattcac aagaaggcac tcggcatgct gacggacccg gacgggccgc
     1261 tcaccgttcg tctgagttcg ctccgactca ctgttccttg gctctggcgt atgctgaagg
     1321 cctcgcgggc cgagcgcatg gcgcctatcg cggacgccat gaaggcgctc tatgcgacca
     1381 cggtcgagct gcacgaggcg ctggcccgcg aagcgggcgt tcccgagctc gtcgtaccgg
     1441 gccgctatct ctacgtctac cgcgatcccg ccaacgccga cgtcgagaag ggcctcggct
     1501 ggcggctccg gcgcgagcgg ggcgccgagc tcgaggtatt cgacggggcg gcgctacagg
     1561 agatcgagcc ggaactctcg ccgatctaca agcgcggcgt ccgggtcggc ccgctcgcca
     1621 agaccaccaa cccgcaccgc ttgaccgccg cctacgctcg cctcctcgag cggaagggcg
     1681 ggagaatcct gaaggccgag gtgcgagaga tcgttccggc ggcgacagcg acggccccca
     1741 tcgccgtcga gacctccgcg gggcggctcg aagccgagtt gctggttgtc gccgcggggg
     1801 cctggtccct cgatctgctc cgtccgctcg gcgtcaagtt ctcgctcatc gccgagcgcg
     1861 gctaccacct cacctatccg gaaccgggct tcgcgctcag ccacatggtg agcgagggcg
     1921 agcgctactt cgccctgacg acgatggaga tggggctccg ctttgctggc acggacgagc
     1981 tctctcatgc cgacgatgcg ccgcgctggc gccgcgcgga cgtgctcgct cgtttcgcgc
     2041 cggagatgtt cccgcgcctc ggcacggcca acccgagccg ctggagcggc ccgcggcccg
     2101 gtacgcccga cagcctcccg gtgatcgacc gtgtcccggg ccagccgcgc atcctgatcg
     2161 cctgtggtca cggccacctg ggtctcaccg gcgggccgat gaccggtcgc atcgtcggcg
     2221 cgctcgcttc cggcgagcgt ctcaatctcg acctggcacc ctacgccgtg acgcggttcc
     2281 agtgaccgcg cgatcccccg ctccatcgga cgaggaactt tcgcagctca atccgagccg
     2341 ctaaaggtcg acgtccgcgc taaaccgtgc acaaaggccg tccgatgtca gagctgccag
     2401 gatcagtcgc cgccacgcgc gcgctccttg agcgcggcgg ctatctcgcc gacgaggcgc
     2461 tctcgacgac ggtcttcctt ggattgaagc tcgcccgccc gctgctgctc gagggcgagc
     2521 ccggaaccgg caagaccgaa atcgcccggg tgctggcgac ggtgctcgag cgtccgttga
     2581 tccgcctcca gtgctacgag ggtctcgaca tcgcctcggc cgtctacgag tggaattacg
     2641 cgcggcagct cctccacatc cgcgcgagcg agggtgcggg gcgcgggggc gacgccggcg
     2701 ccatcgagcg cgacatctac accgacgcct acctcgtgaa gcgcccactc ttgcaggcgc
     2761 tcgagttctc gaaatcaggc cgcgcgcccg tgctgcttat cgacgagctc gaccgggccg
     2821 acgagccgtt cgaggccttc ctgctcgagc tgctcgccga ctatcaggtg acaatccccg
     2881 agctcggcac gatccgcgcc gaggcaccgc cgatcaccat cctgacctcg aaccggacgc
     2941 gcgaggtgca cgacgcggtt cgccgtcgct gcttctacca ctgggtcgat ttcccgagcg
     3001 cggagcgcga gcgggcgatc ctggcgctgc gcgtccccga ggctccgcgc gaactcaccc
     3061 gccaaatcgt cgccttcgtg caggggctcc gtcagatgga cctctacaag acgccgggga
     3121 tcgccgaatc gatcaactgg gcgcaggcgc tgacctcgct cgactgcgtc gcgctcgatc
     3181 cgactgcggt cgacacgacg cttggcacgc tcctcaagta tcaggacgac atcgcccgca
     3241 tccagggcag cgaagccgcc cgcctcgtcg agaaagtgcg cggcgatgcc cgcgaggtgg
     3301 cgcgggctgc gggtgcgggt caatgaggta acataatacc gatttattaa attgttgaat
     3361 ttgctgaatt aatggcgttt gacgacgcgc tgtttgacaa ctagaagatg tcttcgtcgc
     3421 ccgccgggag ggatcgctcc gcctgccggg ctcgtttttt gagcggcgcc gccaatcggt
     3481 aagctgccgc catcgcaaac cgcaggaacc ggcaggagca ctgcccagat gaaaacctac
     3541 aacgccaagc cgggcgaggt ggagaagaag tggctgctga tcgacgccaa agggctcgtc
     3601 gtcggccgtc tcgccaccat catcgccacg cgcctcaagg gcaagcacaa gccctcctac
     3661 acaccgcacg tcgacatggg tgacaacgtc atcgtcatca acgccgacaa ggtcgtgttc
     3721 acgggcaaga agcgcgagga caaggtctat taccgccaca ccggctatcc gggcgggctc
     3781 aaggagctca cggcgcgcca gatcttcgag ggcaagcacc cgacgcgcgt tatcgagaag
     3841 gcggtcgagc gcatgctgcg ccgcggcccc cagcaccgcc agctgatgcg caacctgcgc
     3901 atctacgccg gcaccgagca tccgcatgac gcgcagcagc cgacgaccat cgacgtcgcc
     3961 aagctcaacc gcaagaacgt gagggcctga cgcccatggc caccgagacc aagtcactcc
     4021 aggatctggg cgcgctcacg ggcgctgctc cgccgcccgg cgccgatacg ccgtccgcac
     4081 ccgtctacca gaagaaggtc gacgccaagg gccgcgccta cgccaccggc cgccgcaaga
     4141 acgccacggc acgcgtgtgg gtgaagcccg ggaagggcgt catcaccgtc aacgcgaagg
     4201 actacaccaa gtacttcgcg cgcccggtgc tgcagatgct gcttcgccag ccgctgaacg
     4261 cggcccagcg cctcgaccag atcgacgtca cggcgaccgt gcgcggcggc ggtctctccg
     4321 gccaggccgg cgccgtccgc catggcatct cgcgcgcgct cacccacatc gagcccgagc
     4381 tgcggagcgt cctcaagaag ggcggtttcc tcacccgtga cagccgcatg gtcgagcgca
     4441 agaagtacgg caagatgaag gcccgccgca gcttccagtt ctcgaagcgc tgatcgttcc
     4501 acatatcgcc tgcgagaacg gcctgccctc cgcggcgggc cgttttctgt tggttgcccg
     4561 catgttggag tgaccgggct cttgaccgtc cccaaccagt caacaagcta tccaccgtga
     4621 gttcggcgtg cgattcgggg ggggcggtca tggatcacag gggaatggct cggctcgccg
     4681 cggacccggt ggccgtgcgc gcctgggcga aggcgattgc cggcaatgcc gtggccgagc
     4741 tttccgacgg cgaccgctcg ttcctcgcga cgctcgccga gttcgacggc acaccgccca
     4801 tgagcgtgcg ccagcgcgag tacctcgacg gcctcatcga acgcacggag cgctatccgg
     4861 tcgtcgacgg ctttcgtgcc gaccggctcg tccgtctcat ccatgaagcg cggctcgatc
     4921 tcgacgaaga cgaggaggcg cggatcgcgg acctcgccgc cggcggcgcc ggacttgcgc
     4981 tctcgccgcg ccaatggcgc tgggtactgg cagtctcgcg gcgcctcggc ctgatcgatg
     5041 acccttacat cgcgctgccg gggcgcggct gacacctgtc gcgttcacgg cgtaggcgaa
     5101 aaaatcgctc tgtcggatcc atgactgctt cagagacggg acaactcatc tggagcaagc
     5161 gtcatgatcc tcgccgccac cacccacgag atcgcctcca ccgacgtcgc cagtgccaat
     5221 caggcccgct ccaagacgtc gatcgtcggc tacatcgctg ccctagcgac cgagctccgc
     5281 cgcgcggtga gcgccaatgc cgcctacgag gacctgaacc ggcgctcgga cgccgcgctc
     5341 tccgaccttg gcctcggccg ctcgacgatc gcccgcgaaa tccacaccac ccactacatc
     5401 gactaatcgg atcgctgctg cactgtcatt tttgaacgac gatcatttca gtctgaacga
     5461 tacttcccga tccggggccg gaccgccgag cgcgagccgg ccccaattcg ttgtcacttc
     5521 agggatttga ccgcctcgag cacctcggcg gcatggcctg gcaccttcac cttggtccag
     5581 ctgcgcacga gcttcccggc cttgtcgatg aggaaggtcg aacgctcgac gcccatgtac
     5641 ttcctcccgt acatggactt ctcgacccag acgccgaacg cttccgccgc agccttgtcc
     5701 tcgtcagcgg cgagcaccac gccgaggttg tgcttcttgc ggaacttcgc gtggctcgcg
     5761 cagctgtcgg gcgaaatgcc gacgacgacg gcgcccgccg ccttgaactc ggcagcaagc
     5821 tccgtgaaat cgatggcctc gcgcgtgcaa cccgaggtgt catccttcgg atagaagtag
     5881 atgaccgcgg gcgcgccttt cagcgtggcc ggagagacac ggccgccatc atccgtcggg
     5941 aggtcgaatt ttggtgcagg ctttcctgtt ggcagcatgg tttcgccttg gtttggtcag
     6001 gatcttggct tcagcgtcga gcggcaacgg catcgcgcta cgcgtggccg ccgacgtcgg
     6061 atctgtacca gcgactgttg cggatcggag acgatacgac aatgccgccc gcccgcgagg
     6121 tctgcgacgc gtgtcgcaac gcgaacctcg ctggtgccgc gacttcgact aaccgtgctc
     6181 cgttcggcga cagtatctga tgccgcaaag gggctgtttg caagcaagtg catgcctctt
     6241 tcgacgcatg ccaccgatgg ggacgactga ttgagccgaa caggtccggt tcggccgcaa
     6301 gggccgcacg cttcgccagc gccgtcgacc gggcgcgacc cgacggtgtc cgatcgtggg
     6361 ccaatggccg ccgctgatcc gaggactgcg ccgccaacga gacgcccgcc acctgtcgga
     6421 ccggacggag cgccgcgcac cacaccgatc acacgccccg atgcgtccga gcgcacggcg
     6481 cgcagcaccg gccgaccggc cccgaagcgg aggcgcgggc tcggcgtgag gttcgtcacc
     6541 ggagcgatga agctgttcgt cgttgtcgcc gtgctgttcg cattcggcgc cggcctcgcc
     6601 tacgtgcgcc tccttcacag cccgatctcg gcccgcatgc tgattggccc gatcgagcgt
     6661 gccatcggag ccggccttgc cgatctcacg gtgcgcatcg aggacgtcga gctcgccaca
     6721 ggcgacggca atctgctcga actccgcgtg aagaacatgc gtcttgtcgg tccggacggc
     6781 aaggccgcag ccgaggtacc gagagccgcc gtcgagctca gcacggcgtc gctgctgctc
     6841 ctcgatgtcc agccgcggcg cctcgatctc atcgagccga gcttcaatat cgcctaccgc
     6901 cccgggaaag gtatgtcgct gagcctgaac cctgggccgc tcgacgcacc ggcaagcgga
     6961 tcggtaggcg gaccggcatc cggagcaccg gcagccacgg ccaccgatcc cgccggagag
     7021 agcgaagcaa ccgccgccgc ccgcggcgtg atcgagatgc tgcgcggcgc gcggcgccag
     7081 tcgagcggtg gcggcctcga gcaggtcggc gtccgcaatg ccagcgtcat cctcgacaac
     7141 aacggcatcc gctcggtctg gcgcatcctc ggcgcggagc tgagcctgca gcatcaccag
     7201 aagcgctcag tgctcgtcgg cgagatgcgc atggcctccg accgaagccc ctggacgatc
     7261 ggcatcaggg ctgaggaatc cgacaagacg aagttgctct ccgtcgagct cgccattgcc
     7321 aatgtggtgc ccgaggagat cggccgccat tggcccggcc tcggcgttct cagagccatc
     7381 gagctgccga tgtcaggcaa ggcgacgatc gagatcgacg cgcgcggcga gatcatagag
     7441 gggacgctgt cgatcggctc cgtcgagggc agcgtgagcg gcgcacggcc tcccgtgatc
     7501 gatcagatca agcttgctgc aagctacgcg tcgagctcgg gccgcgtctc gatctcgccg
     7561 tcgaccttgg catcgggggc gtcacggatc accttcgtcg gcgagatcgc accacgcccg
     7621 cccacggccg acgcgccggc atcctggagc ttcgtgatcg ccgccaatga cggcgtcatc
     7681 gccgccgaca cgagcgtccc cacgttgccg gtcgagcggc tcgccatccg tggcacgctt
     7741 acaccagaga gcaatctcgt gcgcatcgac gagatgcagc tccaggccgg caaggcgagc
     7801 gtggcgatgg ccggcgatat ctggttcaag ggcgagcgcg tcggcgcgac cctcgaagcg
     7861 cgttccgcac cgatgagcgt cgcgacgttc cgggcgctct ggccggcggt gatcgcgccc
     7921 aaatctcgca agtgggtcgc ggggcacgtc ggcaaggcgc agatcgagag cttcgtgctg
     7981 aagaccgagc cgtcgagcga tacgcgcccg ggcgccaagc ccctcaaggc catgatcacg
     8041 gccgagatct ccggtgtcga ggtggccgcg cttaagtggc tgccgatgct cgacatcccg
     8101 cgcttgctga tccggatcga cggaccgcag ctcgacatga ccgctccgga ggggcgcctc
     8161 atgaccggcc cgggccgaat cgcgcagatt cgcgccctga aggtcacgtc gcaggacctc
     8221 gatgtcgagg tctcgcaggc cgagctaatt ctcaagggca atgcgagcgt ggcctccgca
     8281 ctcgacctga tcgagcgcca tccggcatca ggattcaacc gggcaagcgt cgggctcgac
     8341 agcgcggacg gtcgtgtcga gggccaggcc cggttcaagt tcccgatggt cgaggacatc
     8401 gatgccgccg acgtccgcat cgagggctcg gcgcgggtca gcgacggccg cgtgcgcaat
     8461 ctcgtcggca agcacgacgt gcagagcgcg gccatcacgt tcgagttcac cgacaaggcg
     8521 atcgacgcca agggccagtt catcgtcgcg ggtgtgccgg cgcggctcgg ttggaaacac
     8581 atcatcggtg tgcctcccga tcgccagccg gagatgaaca tcacggcgac gctcgacaac
     8641 ggtgaccgga cgcagctcgg catcgacacg gctccattcc tcaccggtga gacgaagctc
     8701 gacgtctcgg tgctgaggag cggtcgcgag gctccgaccg tccatgtcaa cgtggacctc
     8761 acgcaatcgg agctcgcgat cgacggcctc ggctggaaga agcccgtcgg catcgcggcg
     8821 cgcatggatt tcgacgtcgc ccgcgggcgg cagaaccgca tcgagttgca gaacctaaag
     8881 gtgaccggcg gcgacatcgc catcgagggc tgggtcggac tcggccccga caacaagccg
     8941 cgggaattcc atttcccgga attctccgtc cagattgtca gccagctcga ggtgcgcggc
     9001 acactcaagc cggacaatgt ctgggacctc aaggtgaccg gtgcccgctt cgacggccgc
     9061 aacgtgttca agtcgatgct ggcggccggt cgaacgagcg agcgccccgc gccacccgcc
     9121 aaggatcgcg cgggcctcga catgacagcc gagatcggcg ccgtgctcgg cttcaacgag
     9181 aatgcgctca aaggcctcaa gctgcgcctc tcgcggcggg gcgagcatct caccgcgctc
     9241 gacatgtcag gcacgctctc gagcggcgag cagctcctcg cccagctcca gcgcgacggt
     9301 cagcgccagc gcctcctccg cgcccagacc tccgacggcg gcaacgcctt caagctgatc
     9361 ggcttctatc ccaacatgat cggcggcctc atgcagctcg aggtcgacct tgacgggcga
     9421 ggtgccgccg acaagacagg cacactgtgg gtgcgcaact tcttggttct cggcgaccag
     9481 atcgtcagcg atttcgtcgc cacgaccgaa gacggcaacc agccgcggga agcgccgaag
     9541 cggccgcgcg agcgggtggt gcgccagcag atcgagttcg agctgctgcg cgttcccttc
     9601 tcgatcggac atggccagtt cgtcctgcat tcggcgcaga ttcgcgggcc gctgatcggc
     9661 gcgacacttc gtggcaagct cgacttcatg gcgcgccagc tcgcgctcaa cggcacctat
     9721 atcccgcttt ccggcatcaa ccgggctcta tcgggcatcc cgatcctcgg cgatatcctg
     9781 acgccgaagg gcgagggcgt gttcggcatc accttcgcga tcgaggggtc gatggagcgg
     9841 ccggtgctca ccgccaatcc catggccctg atcgcgcccg gaatcttccg cgagatcttt
     9901 cagatgggcc ccgaggacac gcgcatcacg ccgcgcgaaa ccccggcccc gaaggcgcgg
     9961 attgagccgc gcgggcgagc ctcgtcagtg ccgccgacgt ctggcccgcc gcccgcgagt
    10021 gcaccgtcac ccgattccgt gagcggttgg acgagcggca ccgaaaccaa gccgcggtcg
    10081 gcgccggcct ccccgaaaaa gtgagccgcg gtttgatgga aatggccggc agctcgatcg
    10141 atcacttgac gccacgctcg ctcgccgaag gcgccgcggc cattgtcgcg accgccgatc
    10201 tcgcggcaaa gatcgcgctc gccaagctga cggccgaggc ttggcgtgac aaccgcctct
    10261 ccctcagccg ccggaccggg atgcccatgc ccgaccggcc cggacgaccg ccgcggccgg
    10321 tgctgctgcc gccgcggcgc atgccgaaac gctcgaccga aggcgagaaa gggcgcttcg
    10381 ctctcatgca tgcgctcgcc cacatcgagc tcaacgccgt cgacatgacg tgggacctga
    10441 ttggccgctt ccacgccgag agcgtgcccg cagccttctt cgacgattgg gtacgcgtcg
    10501 gctatgagga ggctgcgcac ttcgagatgg tgacgcagcg tctcggcgag ctcggcggca
    10561 gctacggcga tctgccggcg catgacgggc tttggcaggc ggcgcaggcg accggccaca
    10621 gcctcattgc ccgcgtcgcc gtcgtccccc tgatcctcga agcgcgcggc ctcgacgtga
    10681 gccctgccct catcgcgagc ctgactgcgg ccggcgacaa ggcatccgcg gccgtcatcg
    10741 aggtcatcta ccgggacgag aagacgcacg tcgcgtgcgg ctcgcgctgg ttccgcttcc
    10801 tgtgcgagcg cgagggccgg cctctggagg cgaccttcca ccgacttgtc agggagaatt
    10861 tccgcggtcc ggtcaaaccg ccgttcaatg acgccgcccg ctcggaagcc ggcctcacgc
    10921 ccggcttcta caagccgctc gccaggctca tcggcgattg agcccgagca gccgttcacc
    10981 aagtgcggcg cgttcgcgta gggcggcgcg gggactgaac tatagtgttg gcgagtttgg
    11041 gggctcgact ggctttcagc agtagagacg agctgccgcg ctgaattcgc acgggcgctc
    11101 aagattcgcc ggattgttgc gcaaggtgta cttaccgagc gcgtcgagtg tgctaaactc
    11161 gcaccaaatg gacccgccgg taaggcgtgg ttggggaccg tccaggatca aagccgctcg
    11221 tctgcgtacc aggaggttgg ggacatgttc acgaagaagc ctgatcggga tactccgccc
    11281 ccgatcgatc tcacaaaatc ggcgccgccc attcctccgc ccatcacctc ggccccacgc
    11341 ccggcaagct cacgcccatc cacgacgtcg tccgtcatcg ggcctgacct taccgtcatg
    11401 ggcaacatcg tctccaaagg cggggtgcag atcgacggcg agatccaggg cgacctgcat
    11461 gccatgaacg tggtcgtcgg cgagaacgcg cgcatcactg gcggcatcat cgcggacgag
    11521 gtgatcgtgc gcggcaccgt catgggctcc atccgcggtc gctccgtgtc gctgcaatca
    11581 tcgagcaagg ttgagggcga cgtctatcac cagtcgctgg cgatcgagca aggcgcctat
    11641 ttcgagggaa aatcgcgccg ttccgaggat ccgctcgcca gcacgacgcg cccggacggg
    11701 ccgctgacga atggcgctgc ctcaacctcc tcctcgacac tgccgagctg aggcccggcg
    11761 cgcgaggcca tggcgacgca ggtccgagat gatcgggccg cgcgtcgtct ttgccctcct
    11821 tgtcgttatg atcgccgtcg ccggctgtgg ccgggcagcg ccggacgcag gcgaggcgcg
    11881 cctctgccgc tccgtgatcc cggcgctcga ggtgatcagc gcgcgaccgc aacgagagcg
    11941 tgtgcgcatc atcgacgagc ggcgcatcgc cgggcatgca gccgtcgaga tcacctatcg
    12001 ctacgtgagc gagcccccgc gcatcggtcg ccgcatcacc tgtacgttcg agcggcgcgt
    12061 cgctgccgcg gaggcgcaac cgcgcgacct gatcgaggtc cggctcgagc agcggccgat
    12121 gtcggcgatc cgcctgcatc tcttgaagcg cttctggctc gaggcgaatg catcggccgc
    12181 gatcgacccg gagccgatcc gcggcgcggc ccgggccccc gagatcgggc cgggactcgc
    12241 attcaccttg caacagacgc tgcttaccgt ccccggcatc gtcaacaccg ccctcctcgc
    12301 cggcgcattt gcgctcgtct acgggctttc caaccgcatc aatcttgcct ttggcgtgat
    12361 ggcgatcgtc ggtggatacg gggcgctgct cggattgctc tcgacggttg ggcttgccgg
    12421 cgccacgccg gccggtctga tcgtggccct ggcgtttgcc gtctggtcgg cgtccgtgac
    12481 gagcgcgggc acggccgcgg gcctcctcgt gccgctgcag cgggagcgaa gccagatcat
    12541 gctgattgcc ggcctcggcc ttgcgatcgc gctcgaggag ttcatgcggc tcatccaggg
    12601 cgcgcgcgtg cagtgggtgc cgccgatctt caatcagcca ttcgcgctcg cccgggccga
    12661 tgacttcgtc gtggcggcga cggccctctc cctcctcgtc atcgccgttg ccgcgctggc
    12721 gattgccgtg ctcgtgctgc tcatgcgccg gagccgcttt ggccggcggt ggcgggccat
    12781 ttccgacgat gaacttgcgg ccggaatggt cggcatcgat gcacggcgca ctgcggtcgc
    12841 gacctatgcg ctggcgggcg cgctcgccgg cgttgccggc ttcctctcga cgctgctgta
    12901 tggcggagtc ggtgcatcgg gcggcatgct gctcgcgctg aaggcgctgc tcgcagccgt
    12961 catcggcggc atcggctctg tcgggggcgc gatcctcggc ggtatgctgc tcggcgtcat
    13021 cgaagggctc tggtcggcgc tgctaccgat cgagcatcgc gacgcggcga tcttcacgct
    13081 gctggtcgcg gcgctgatcc tgtttccggg cggactcttc gatcgccgcg cccgcgaggt
    13141 gcgatcggcc tgatatgggg ccttacgtgg ccggcatggc tccgcgccct cgatttctac
    13201 cgtggatcag ccttcgagct ccacgtgacg cgcgcgcgcc gtcatggcga gcgcgatcag
    13261 gaagctcagc agcatgaggc cgagcccgac gagcccgagc cagcgctcgc cgccggcggc
    13321 ataggagacg aggaaggccg cgcccgaggc attgatcagc cagctcgaga tgaagaggtg
    13381 gatgcggtcc ggaaagatcg cctcgacgag cgaggagagg ccgaccgccg tcagcatgcc
    13441 gagcacccag gcgttcttca tgtcgaagcc ggagtgcgag aagaactgcc gccacatcgt
    13501 cgcgatcagc acggcggcaa tggcggcgac caggaaattc agccagcgca tgttgcgtgg
    13561 ggtgcgcagc gccggcttgc gcgggggcgt cgccggggcc ggggcggttg cggtcacatt
    13621 cggatcagtc gtcatctgag tacgggctcc tggtagcagc gcgtctgtcg gcggcacacg
    13681 gagggtgtgc caggtcttcg cccgtcgcaa tcctgcttcg tcgcggccta cttcgtcttc
    13741 gcaagcgctg tcagggtcgt cgggccccct gccgggcagg atgacacacg gccggtctgc
    13801 gatccaggct tgggcgggct tcccttggcg aagccgtcga gcacccgctc gaagagctca
    13861 ggcgtcagat cctcgtaggt gtccttccag atctgcacca tcggcgcatt cgcgcacgag
    13921 ccgaggcatt cgacttcctc ccaggagaaa tcaccgtcgg cggagagctt gccggcctcg
    13981 ccgttgatgc gccgcttgca gacctcgatc agggcttcag agcccgagag catgcagggc
    14041 gtcgtcccgc agacctgtac gtgcgccttg cgaccgacgg gcgagagttg gaacatcgta
    14101 tagaaggtcg cgatctcgta gacgcggatg taggccatgc cgagcatctc ggcgacgagg
    14161 cggatggctg gttcgggaag ccacccggcg tgctgctcct gcgcacgcca gagcagcgga
    14221 atgacggcgg acgcctgccg cccgtccgga tactgcgcga tcttggcccg tgcccaggcc
    14281 tcgttctcgg gagtgaaggc gaagctcttc ggctgcgttg gatcgaggcg gcgaacggcc
    14341 atgaggcagg tctcacccaa ttaggggcca cgtttcgttg gtccgggctg aggccggacc
    14401 accgtcgtga tatcgtcgta tcgcctggcg ggcaaccggt cgtcgctggt tgcggccctc
    14461 gggcgcgggt cgaagggcaa tggcgttgag atccaagaga ggaccagcgc cagcgtcgcc
    14521 agcggcgcaa gcagcagcga ggcctgcgcg cccaccctga agatcgccgt ccaggcgagg
    14581 gccaggagag cgccgagcac cggaacgatg aggcccgtgc cgccgccccg ggcgagcccg
    14641 aagacgagcg ccatggtcat ggcgatgacg ccgagcgcaa acggcaggcc cggccggttc
    14701 gcctgccagt cacccatcgg ccagccgttg atcagggcga accagcgata ggtgaccagc
    14761 gacaagcccc aggcgaatgc ggcgaccgca agccagagaa tgagatcgac cgacattcca
    14821 tgctcccttc gatacccggc ccgcgacaag gcggcggcag cccgcggtgc ccggccggca
    14881 ttcgctgcac catccgccat cggccggagg ccgtcacgct gctctgccgc gcatggtaag
    14941 cgattgccgc gtcgaaggaa atcactcgtg ttcggtagcg gtatcgagag ttgggtcgcc
    15001 cctgtgaaag gaagcgacag ccgcttgtcg caaaggcgcg gcgctgcgag gcggccctct
    15061 ccgcgcaggt gtccacaggc gcgtgtggat aaccggcccg ccacaatcca aatgtcgccc
    15121 caatcaactc cggatattaa taattggacg acggattgtc cccagggcca cgccaactcg
    15181 atccccactg tggcccccga cgccgaaagc tccagccgcc gcgctggagc tctttttttt
    15241 gtgcagtgcg agttgatgct gcagtgccaa cgtgttgtaa accttactga gaaccccgcc
    15301 ttaatcctgc tctgctaact tcagttggat catggagagg tatcgtcacg ggccagtgcc
    15361 cgcggcgagc gttggcgaag gactgcacca tgatcaacgg cagcaagatc ggcattgcgc
    15421 tcggtggtgg cgccgcgcgc ggctgggcgc atatcggcgt gctgagggcc ctggtccagg
    15481 cgggcatcaa gcccgacata atcgcaggca cctcgatcgg cgccgtggtc gggggctgct
    15541 tcgcggcagg ccgactcgac gagctcgagg ggttcgcgcg agagctcaca cggcgcaagg
    15601 tcttcggctt cctcgatttc aatctcgcgg gctccgggct catcacgggg cagaagctcg
    15661 tcgaccggct cgaagcctac ctcggcgacc tcgatatcga ggatctgccg gcgaaattcg
    15721 tctcggtcgc gaccgagatc ggcaccggcc acgaggtctg gctcggcagc ggcaagctcg
    15781 tcgccgccat gcgcgcgtcc tacgcgctgc ccggaatttt caagcccgct tccgtcggcg
    15841 ggcgatggct gtttgacggc gcgctcgtca atccggttcc tgtctccgtc tgccgtgcgc
    15901 ttggcgcgcg ctacgtgatc gcggtcaacc tcaattccga cctcggcggc aagggcaccg
    15961 tgatccaggc cttcgacacc tcggaggaca ccgccacggc gcagcccgct gaacccgagg
    16021 gccgtggcgc gcgccgcctg atcgcccgcc agctgctcgg cccgagcagc gatgcaccgg
    16081 gcatctcgac ggtcgtggtc gacgctttca atatcgtcca ggatcgcatt gcccgctcgc
    16141 gcctcgccgg cgatccgcct gacgcaatga tctcgccgaa gctcgctagc atcggcctct
    16201 tcgagttcca ccgggcggac gaggccatac aacagggcgt gatggcggcc gcgcgtgagg
    16261 tcgaggacct cgggcgcgaa ctcgcgcttc gccgccgcat cgccgagaag ccgctgccgg
    16321 cgaccatccc cgcctatcag agctgacgtc tgacgtcgat ttcaggccat cgcgaccgcg
    16381 ctgtccgcga cgaactcgag cggcgtccag ttgcgcgagc gcacccagtc ggcgcgcggc
    16441 ttttcgacgt cgcggggccg gtttgcgcag gtgttgtagc agaaataggc ctgccagcga
    16501 tcgttggccg agaggttgtg ccccgaggcg tgcatgaggt tgcaatggaa gacggtgagc
    16561 gagccggcct tgcccgtgat cgcgaccggc tcgccgtgct cggcgatcat gcgcttcatg
    16621 tcggccggcg tcaccgacca caccttgtag accgtcgacg tatcgaaata cgggtcgagc
    16681 cggccgtgca cgtggctgcc ggggaggaaa tagaggcagc cgttcatctc cgtcgccgga
    16741 tcgaggccga ccatgatcgt cagcaggtcg ggccgcacga tgccgtcgtt ggcccaggac
    16801 ccatagtcct ggtgccaggg ccacaccgag ccctcgatcg cggccttgat gttcaccttc
    16861 gtgtggtgga ggtagagctt gtcgtcgcgc aggagttggc gggcgaggcc gagcgtgcgg
    16921 ggcaggcgca ccacgtcgtg aaacaccggc gaggcggtcg gcccatcgct ctcgtgcatg
    16981 cggaacatcg ccttggggct gcgctccgag ccctcgcgca ccaccatctc gctctcgatg
    17041 cgcgagacgc gctggacctc gcgccgcatc gcggcgacct cggtcgccgc gaagaactct
    17101 gggaaatgca ggaagccgtc gcgatcgtat tgcgcgatct gcttgtcggt gagctgcatg
    17161 gcatcctcgc tcgaacgatg tggatcaggc accgatatcc ttgggcggca ccatcgcggc
    17221 caaacgggca agatcaccct cgcgctcgat ggccgctggg tcgagcccga gggtgggtcg
    17281 cgtttcgctc gcatcgacga gatagtgccg caggaacgcc gccgcgcgcc cctccgcaga
    17341 cggtaccgcc gcgatggtcg cctccacgct ctcgccgtcg cgcgccggtc tcagcgtcac
    17401 gaccgcacct cgcgagcaga ccgcctcata gccgcccctc gcaaggtcct cgggcgagag
    17461 gtcgccggcc aacgcctcga gcgagccgaa gagtgtcacg tcgtccggga cggacgcgtc
    17521 gtccggattg tgattgagca tcacgaacca gcgtgactgg gaggagtcct gcctgagcgc
    17581 cgactgaacg gacttcaaga gctttccgaa cacgcgatgg ctccttgccg gtcagacacg
    17641 ccaatcacat catgccgccc atcgccggcg cacccttgtc ctcgccctgc tgcttggcaa
    17701 agatcgagga atagccgcct gaccaatccg gcggcagcag gcagggccgc ggatcgtgaa
    17761 aggcccagcg ggcgggattg ccacgcacga tcgcgcccga atagcgcgac gggatcggat
    17821 tgaccgagta gggcatcgcg ttggcgttcg agaagacgtt caagagcagc ggccgcgcga
    17881 tgggcgagtt gttcggctcc gagtaatgca gcgagcggca attgtgcacc gagatcgagc
    17941 cggccggcgc ctcgagccag acgaccttct tcaggtcgag cttggcgacg tcttctttgg
    18001 cgaggcagcc cgtccagttc cccttgtcgt cgtactcgct gtagatcggg ccattgtggc
    18061 tccccgcgat gaagcccatc gggccctgct cgcgattgca gtcgtagagg taggtgccgg
    18121 cggtcgcggg cgaatagttc gtatgcggcc aagccgggat gtcctggtgc cacttcacct
    18181 cggcgccgcc cttcgcccat ttgaagttga gcttggagtg atggaatttc acgtccggcc
    18241 cgacgagatc ggccatgatg tcggggagca cggacttctc cgaggaggcg tactcccagt
    18301 aggtcggatg atgatcgttc ggactcgaaa ggcgtctcag ccgcgggttt tccggcgtgt
    18361 gatccggctc gatgtcccat ttggcgtcgc gcttcgtgat cgcgcggctc tcgtccacca
    18421 tcttctgtgt gacggcgcgc agccgctcca cccagtcggc gggaatgacc cgctccagca
    18481 tgatgtgacc atcctcgaag tagcgctcac gctgcttctg cgtgaggatc ttcggcttga
    18541 tggacaggat ctgctcgggt gtcatggggg gctccttgta ggaggacagc gcctgcttgg
    18601 gctggcggtg gggcgtgagc acgcccgcca gctccagccc aaccggtcgt tcagccaggg
    18661 cagtcgttca gccagggcag tagaagttcg ggtgtctgcc ttgcctcggg gcaccacccg
    18721 gacatttgca gatcgagata ttattggctg gattcgtcga gatcgtgccg cccttacatt
    18781 cgatctgcga cctggtcttg ccacaacgga agtccggatg agtacccttc acaatatagc
    18841 caaaaccgca tcgacactct gctcgtccgc tcgctgagat gccgatcgtc ccaccatcgc
    18901 atgtgatctg aggagcggca ttctttttta cgagaggcgg aggtgtcagg ggcgccggat
    18961 tcttgttctt gtctttttcg caattgaaga agaacgttcg accgtcctgc cctctgccga
    19021 cgtcgcacct tccgccgcct ggatgtgtag aataaatctg ggcgccggcc gatacgaccg
    19081 ttgccaaaag tatggcggca gtgagtgtcg agattttcag caaggcaggt ctccgttggt
    19141 tgcaaggtgc tggatcaata gtcatgaaac tcgactggtg cgctcatgca gcggctctga
    19201 cgtttggtgc ccgctcgcgg cttggatcga gaccaaacgt cagcgcccga acactggtgt
    19261 ggcgtcgcgc cctagttgac cgatggtgcg gtaaggctcg catcaaccaa ggcgcgatac
    19321 acgccacacg aagccgatga taccgctagt ggccctcatg tcgcgccaaa gatcggaata
    19381 ggcttcggcc atgatgcgat tttggcgcga cgggccattg ggttcccttc tgactgacca
    19441 ctctcacatc atcccgccac ccatcgcggc ttgcggcgga ctcttcgacc aatcctcctt
    19501 ctgctggagc gcgaagatcg aggtatagcc gccggaccag tcgggcggca gcaggcaggg
    19561 gcgggggtcg tggtaggccc aggtcgccgg cttgccgcgc acgatcgcgc cataataacg
    19621 cgactgcagc gggttgtagg tgtagggcat cgcgttcgcg gccgagaaca cgttgagcag
    19681 tagaggccgc ggcgtctcgg accagttcgc tttcgagtag tgcagcgtgc ggcagttgtg
    19741 gatagtcacc gagcccgccg gcccttcgag gtagaccacc ttggacatgt cgatccggcc
    19801 ggcgtcctcc gcactcatgc agccgaccca cttcccgtcc ttgtcgtact ggtcgacgac
    19861 cgggccttgg tgactgccga cgagcacgcc gagcgggccc tgctcggcgt tgcaatcgta
    19921 gatgtaggtg ccggccgtgc agggggaata gttcgtgtgc ggccagaacg agatgtcctg
    19981 gtgccacttc acttcatcgc cccccttcga ccatttgaag tttagcttgg agtggtggaa
    20041 cttcacgtcc gggccgacga ggtcggcgat ggcatcgggt agcggactgt cggcggccga
    20101 cgcgtagtcc cagtaggtcg ggtggtgatc gttcatgctc gagagacggc gcagccgcgg
    20161 cgcctcgcgc gtgtggccct tgtcgagatc ccagaccgca tccgacttgg tgacgccgcg
    20221 gctcttctcg accatggcct cggtcgcggc cttgagccgc tcgacccagc tgtcgggcag
    20281 gaacttctcg agcagcagat agccgtcacg gaaatagcgc tcgcgctgct tctgggagag
    20341 gaccttgggc ttcagcgaca gaacttgctc gggcgtcatg aggcctccgt catctgcgct
    20401 cgatattagc gggtccagtg atatatcaga gcggtatgag acgcaagctc agctaccgac
    20461 ggatcgggcg gcgtgtacgt gcgtcgcgct gcgactgcgc cgcaggccca gacccgcctg
    20521 gggctatgac cgcccagtcc cggccttatt tggattgcgc tgttcttgtc gggcgacgcg
    20581 gtgcccgtca cagaatgaac ttcgacagat ccgcgttctt tgcgagttcg gcgatgtggc
    20641 gcttcacgta ggccgcgtcg atcgtgatcg tatcgccgct gcggtccgcg gcgttgaacg
    20701 agatgtcgtc gagcacgcgc tccatgatgg tgtgcaggcg gcgggcgccg atgttttcga
    20761 cggtggtgtt cacttcggct gcgatgtcgg cgagcgcatc gatggcatcg gcggtgaaga
    20821 cgagcgtcac gccctccgtc cccatcagcg ccacgtactg cttgatgagg ctcgcctcgg
    20881 gctcggtgag gatgcggcgg aagtcctcgc gcgtgagcga cgtaagctcg acgcggatgg
    20941 ggagccggcc ctgcagctcg gggagcagat cggcgggctt cgccacatga aaggcgcccg
    21001 aggcgataaa caggatgtgg tccgatttca cggggccgta tttcgtcgag acggtggtgc
    21061 cctcgatcag cggcagcagg tcgcgctgca cgccctcgcg cgagacatcc gcgccgacgc
    21121 gcccgccgtc gctccgcgag cagatcttgt cgatctcgtc gaggaagacg atgccgttgt
    21181 tctcgaccga gcggacagcc tctgcgaccg tctgctcctg gtcgatgagc ttgtcgctct
    21241 cctcgccgat gagggcggcc tgcgcctccc gcaccttcat gcgacgtgac ttctggcggc
    21301 cgccgaaggc cttccccagc atctcgccga tctggatcat gccgacctgg gagccgggca
    21361 tgccggggat gtcgagcgtc ggcatctgcg gcgaactgtc ggcgaccttg atctcgatgt
    21421 cctggtcgtc gagcgcaccg gcacggagct tcttgcggaa gctttcgcgg gccgccccct
    21481 gcgtgtcggc gacgaagaca tcgaggatgc gctcctcgac ctgcttctcg gcctgcgcgc
    21541 ggacctcctt gcgtttgagc tcgcgcacga gcccaatgcc gacctcgacg agatcgcgga
    21601 tgatgctgtc gacgtcgcgg ccgacatagc cgacctcggt gaacttggtt gcctcgacct
    21661 tgatgaacgg cgcgttggcg agccgcgcga gacggcgcga gatctcggtc ttgccgcacc
    21721 cggtcggacc gatcatcagg atgtttttcg gcagcacctc ctcgcgcaga tcgtcggcga
    21781 gctgctggcg ccgccagcgg ttgcgcagcg caatggcgac agcgcgcttc gcggggagct
    21841 gaccaacgat gtagcggtcg agttccgaga cgatctcgcg cggcgagaaa ttggtcattg
    21901 cgataccttc gggttggagc cgctcagccg gccttctgct tcttccagac ggcccatggc
    21961 gcgcgcttct tgagctcggc ctcgttgcat tgtgcagccg gcgccgacgc gagcacgatc
    22021 atggcatcgt cgctacccgt gagcgtcccg cgcttcgaaa cctcgtcgca ctcgatctca
    22081 aaggcgcgag tgtagccctt gagggtgacg cgccccgccc cgtcgatcga gagcgtgatc
    22141 ggctgagcac cgcagcctcc gcagctcgtc gtcccggtct tgcggtcgag attgcagaac
    22201 gcgctctcca ggaacacgac attgggatgt tggcggaagg tcgcggcgat cgtgaagctg
    22261 cggtaactgt cgggcggatt ggcgccgttg tcgcgggcga tgtcctcgcc cgtgcgatag
    22321 aggcgcatgt ggatgacgtt ctggcgcggg tgggcgcgca gatgctttgc gtcgtagtgc
    22381 cgttcgaggc atacggctga gtccttcggc aggatgcgat cgaacggctc gggatcggcc
    22441 gcgagcgccg cgctggcagc gacgctcgtc accactgcaa cgccggcaag cgggcgcaag
    22501 ctcacgcgac cctccgcggc cgcaggcggt cgataggccc gagcggcggg atcacggtcc
    22561 agatgccgcg gcggacgagc tgccagccga ctccgagggc gacgatgccg gtgccgactg
    22621 cgatcggcac gaggtaaccg atctgcgcgc cagtgaagcc cgcctgcgac gccgcgacac
    22681 cgattgccgt gatgagatag ctcagtgccg cgaccaggat ggcacgccgg tcgacgatga
    22741 tggcgatcag cgcgagcacg ccgaatgtca caaacaccgg caccacgccg ccggtgctct
    22801 tccaggcgaa gatcgacatc aaggcatgca cgatcatggg cgccgcgacc atgtgcagcc
    22861 agaagccgca atcgctgcga ttggtggtgc gctcgggatc ggtgaggtcg aagacgagcg
    22921 cgacgacgaa aatggcgatg ccgagcgcaa ggatgaggcg gttgacgttg tcccagttga
    22981 tggtctttgc agcggcgctc cagaagaact gcggctggta ggctgcgagc gtcccgataa
    23041 tgaccgcgac agcggcgagc gccaggatga gcagcgagaa gggaaagcgg aagcgcgcat
    23101 agtgtgcgcc cgcgaccgca aacgtggcgc ccggaagcag cagcagcacg ggcggcatcg
    23161 aaacgttcgc ctggttgaaa tagcgcacta gcgtcacgcc accgcgcgtc aggtggtcga
    23221 tcagcggcac gatggcgatc ggcgccagga tcgcgagcgc gatgacgatg acgatgctcg
    23281 gcgccagcat gcggctccgc gcggtcagca cctcggcgag aacccacagg atcgccatgt
    23341 agctcgcggc ccaggcggcc gtggtgccga gattggtgat cgcgttggcg aagttgcgat
    23401 ttacggtatc gacgccgagc gacacgagca tgccgatgcc gagcaggatg acgccgaggg
    23461 cgacgaagat ctcgttgaag ccgttggcga tgcggaaccg ctcttcgccg ggctcggtcc
    23521 ggtcggctgc cggcgcccag gccttggaca gcgggacttt tgccggggct cccgtgccca
    23581 ccgcgagccg ccagagctgc tccgcctgct ctgtcgtgac gatgcgctgg gcgacggcat
    23641 cgtcgagatg tcggcgtgtc agcatgtggg ctccacggtg aaggccgcaa tcgagattgg
    23701 cacggcgcgg gcagatggat tgcccgtgat atcgacgccc cgatgtctca cttcaagtcc
    23761 ctggcgcgct cgcgcagcac gaacttctgg attttcccgg tcgacgtctt cggcaactcg
    23821 cagaatacga ttgtgcgcgg gatcttgtag ccggccatat tggcacggca ataggcgatg
    23881 acggcctcag cggttgtcgg cctggcgccg ggcttcagcg tgacgaaggc gcacgggctc
    23941 tcgccccatt tctcgtcggg gcgcgcaacg accgcggcct ccatgatctc ggggtgccgg
    24001 tagagcacct cctcgacctc gagcgaggaa atgttctcgc cgccggagat gatgatgtcc
    24061 ttcgagcggt ccttcacgtc gatataggaa tccgggtgcc aggccgcgag gtcgcccgag
    24121 tgataatagc cgccggcaaa ggcttcctcg gtcgccgcct cgttaccgag atatcccttc
    24181 atgagcgtat tcccgcgcag catgatctcg ccgatggtct gcccgtcctt gggcaccggc
    24241 gcgagcgtcg ccggatcggc gaccatcatg tcgtcgatgg cgaccagcgg gatgccctgc
    24301 cgcgccatca gcgcgtagcg ctcggcgatc gggatcgtcg aatcccactc cgagttgtgc
    24361 gcgcagaacg tcgagggccc gtagctctcc gtcgtgccgt aaaggtgcag cacctcgaag
    24421 cccatgccct ccatcgcctg gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnncgcga gggggcgctt cacgctctcc ggcgcatgga cgagcatgtt gagaacgatc
    24541 ggcgcgccgc acatgtgtgt gacgcgatgc tcggcgcaga gctcgaagat gcgcttcggc
    24601 tcgacacggc ggaggcagac gtgcgtcccg cccgaggctg tcaccgccca tgtgaaagtc
    24661 cagccggagc agtggaacat ggggagcgtc cacaggtagc ggctctcgtg gtcgagcttg
    24721 aaagtcagcg cattggagag ggctgcgaga taggcgccgc gatggctgta aaccgcgcct
    24781 ttcggattgc cggtggtgcc cgacgtgtag aggaggcaga tgctctccca ctcgtccgcg
    24841 atgccgggat agtcgaaggc cggatcgctc gaggcgacga agccctcgta ctcgacaccg
    24901 ccgaagctgt tggcacctgc cgtctcctcg tcggcaatat cgatgacgat cggacgcttg
    24961 tcgccgagga gttcgagcgc gggcgcgatc gtcgcgtgga attcgcgatc ggcgaggaag
    25021 agcttcgccc cgccatgggt gaggcagaag gcgatcgccg ccggatcgag gcggatgttg
    25081 atcgggttga ggaccgcccc gatcatgggc accgcgtagt gtgcttcgag catcgccggc
    25141 acgttgggcg ccaggatcgc gaccgtatcg ccggccttga tgcccgcttt cgtgaggctg
    25201 tgcgcgagac gccgcgcccg tgcaaagaac tcgcgatagg tgtagcgccg tgcaccgtgc
    25261 gcgacggcga tacgatcggc aaacaccgaa gccgcgcgct cgagaaagct caccggcgaa
    25321 aggggcacga agttcgcagg ccgcttcctc agctccggat gctctgttcc cgccattcct
    25381 caggcctcaa tcaagcttta ctttgacgtc gaccggccgg ctcgcatcgt gcgtccattc
    25441 ggccatggag ccatcgtaca tgcggacgga cttgttgccg agcaactccg aggatacgaa
    25501 ccagccgagg ctcgcccagt gcccggtatt gcagaaggtg atatgcgcgg ctgtcggcga
    25561 gatgctctgg gcgcggtaga gcttcaggag ctggtcgcgc gttcggaaac gaccgccacc
    25621 gttctccgtg agccagccct cgggcaggct caccgcgccc gggatcgtgc ctgcacgctt
    25681 ggcggccggg ctcttgttga ggcccacata gaagtcgtag gggcgattgt cgaccatggg
    25741 cgtgccggcg gcagcggccg acttcacctc ctccgcggtg acgatcatct cggcccgaaa
    25801 ggccggcttg aaggtcttgg ccacgggctt cacatcgtcg gtcgcgagcg ggttcactgg
    25861 cttcttggtc tgcgcgtcga tcgccgaggc ccagccgatg aagccgccgt cgaggatcga
    25921 caccttgtcg tggcccatca ccttgaaggt ccagtagatg cgggtggcgg tgccnnnnnn
    25981 nnnnnnnncg tcgggcacga tgatcacgtg gctgtcattg tcgatgccga gcgagccgat
    26041 gagcttagcg actttctcgg gtggcggcag catgccgtcg acgccggcgg cgcttttctc
    26101 gcgccagccg tcctttccat agtcggtgaa gaccgcgcct ggaatgtgcc cgacgaggta
    26161 ggcggctttc ggcaggcctc gcgcctccaa cacgaccaag ccgggcttgc cgagattggc
    26221 gcgcaggaac gccgcatcga cgagcggcgt catggatggt gcggccgagg tcaggcccgc
    26281 ggcgaagact gcgaacgtac cggcaatcat ggcttgggca acacgtcgag tgagcatggc
    26341 ctactcctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctg gctgccacga aaggccaagg
    26401 atcgcggcaa gggatgattc accttcaacc cggttgcgcc atcaacatgc gagatcgacg
    26461 tgctagtcac gccaccagcc gacggctaga gcatgggcct tgatgtgcag gggtcgtgct
    26521 ggcgaccaac ggggacaagc gatgcaggca ttgaatctcg ccgacagtga cgacgaaagt
    26581 ctgcgcgcgc tcgatttcat cctgcagggg tgggacgcgg gctcggaagc aggcattgcg
    26641 cccgagttca tggcctatgc ggccctctac gccggtctca ccgatctcgt cgggacctat
    26701 ggcgaggatg ccgtcgtgaa gctcgtcacc ggccttgtga cgcgcgtcaa agagggcgag
    26761 ttcacgctcg tgcgcacgcg ccagtaggcc gtcagcgggg gatggatgcc ggggccggca
    26821 ggccgttgcc gaggggcgag gactgcttga ggttgaagtc gtaaggattt gttacccagc
    26881 ccgtcgaaac ctgagtgccg gggctcatct ggcgctcggc cgattgcgcc caggcgaagc
    26941 ggcggacatt ctgcgcgtag ctgtcgagaa gcggtgaacc gattcgcatg acaccatcct
    27001 gagacgtgac gggaagagat tacctcacaa gtattgttct ccgtttatca gggtcgttct
    27061 agttttaagt aaactggggt aggcggatcg aatttacggg ggatgccggc atggcaggga
    27121 cgaacctgga cgggggcggc acccaccaac ctggacggcg gctcgggctc ctccggctcc
    27181 tcattcttcc cctcgtcatc tcgctcgcgg tgatcgccgc acaggcattg cgaaagccca
    27241 tcaaggcgcc gttcgcggac gagttctact acctgacgat cgcccgcgac ctctcactgc
    27301 atggcgtcat cacggatggc cccttcaagg cgcggctgcc cgacctattg ctacccgctt
    27361 acgtcaccgc tgacgagcgg cccgaggcct cgaggcccgg ccgcgtcttc ggccccgcct
    27421 atccgatcct cgtctcctcn nnnnnnnnng tcgtttttcg atccgcgcct cagagaatcc
    27481 gtgcgctgcc acgtgaaggc cttcggcggt agccaggccc attgcccgaa cgcgtttgcc
    27541 acattgatcg cggcgaacat cgccatcgcg acggtgagtg ggctcgccat cttcctgatc
    27601 ggctggtcgc tcgtgcgctc cgaggccttg gcatggggca tgttggcaat cgcgctcgct
    27661 tcgggcgagc tcggcttcta tgcgcgaagc tacctctcgg agacgctgac gatcgcggcg
    27721 atgctggcgt tcctgctgtt cgccgtgcac gcgatgcgcg attttcgccg ccacgatttc
    27781 gcactcgccg gcgcgtcact tgccatggcg gcgctcaccc gtccggcgta cctctatctc
    27841 atctatggcc tcgccgccct tctactcatt cttgccgtcg caggacgccg gttcgtgccc
    27901 cgcatcactg tcgttcatgc gctggtcttc gggggtgcgg ctatcctcgt gctctccccc
    27961 tggctcgtcc gcaatctggt gcagttcggc gatccggcta tctccaaggg ctatgccgag
    28021 atcattctca cgcagcgcct ttcctataat cgcatgagct ttgccgagtg gggcgtcgcc
    28081 tggatctatt ggctccccga cttcggcgac gggctcgccc gcaaactctt ccccgaagca
    28141 ctctatgcca agctcggctg ggacatgccg acgagctact tctacgaggg cggcagcggc
    28201 gtgttcctcg acaaggtgat ggccgaagcc ggatcgaagg aactcctcct cggccatctc
    28261 gtgcgcactt atctcgttgg cgatgccttg aaacatgcac tcgtgacgct gccgctcaca
    28321 atgcgcgggc tcggcgttgc gaagtatctc tccgtgatcg ggctgccact cgtctggctt
    28381 gtggggcggc gcatgcacgc gcgcggattg ttgcttccgt acctgtcgct cgcgctgccg
    28441 ccactcggca tggcgctgtt ccacggcttc gtatcggtca acatcgagcg ctacaatgcg
    28501 ccgatcatcg cgctctatgc cttcaataca gccttcgtcg tgctggccct catcgagacg
    28561 cgccggcatc ggggcggcat cgcgcaggca tgagcagcag gctcacgatc gccgtt
//
LOCUS       DAORNH010000019        27972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1294_length_27972_cov_2.613461, whole genome shotgun sequence.
ACCESSION   DAORNH010000019 DAORNH010000000
VERSION     DAORNH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 27972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27972
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1294_length_27972_cov_2.613461"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..456)
                     /locus_tag="PK264_03605"
                     /pseudo
     CDS             complement(196..456)
                     /locus_tag="PK264_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            658..>845
                     /locus_tag="PK264_03610"
     CDS             658..>845
                     /locus_tag="PK264_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRD75014.1"
                     /translation="MAAGLALGMAVVNSFARFAYALVLPAMRSDLGWSYTDAGALNTA
                     NAIGYLIGAAAAFRSVRRL"
     assembly_gap    846..992
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <993..1796
                     /locus_tag="PK264_03615"
     CDS             <993..1796
                     /locus_tag="PK264_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HRD75015.1"
                     /translation="ASVFPGRGAASARAIAIYFGGGGFGLLMSAITIPPLLAMAGDRA
                     WPKAWLLLGAMSAAASIAATLAARRVAAPAPVAAPVAWAKLPLLATFLAYACFAFGYT
                     AYMTFIVAFMRDNGAGSVEIGLMWAMLGLATLAGPRIWSGPMSSWPPGFALAGVLATI
                     GIGATLPVIVATPAVMIVSAALFGAFFMTPASVTAIVRTALPPVVWGEAVAAFTLMFA
                     LLQCFGPILTGLLADLTGDLEDGLSVSAAVLMAGAALALLQGLEARPRR"
     gene            1991..2671
                     /locus_tag="PK264_03620"
     CDS             1991..2671
                     /locus_tag="PK264_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRD75016.1"
                     /translation="MAFALAAALALVVWSLAPGQPEARSAADIAGMARVLDGDTVEIS
                     GVRIRLEGIDAPEAAQRCTHRVIGTWACGAAATNELVRLVDGKPLTCTPLGTDKYGRT
                     LATCHLGTLDVNAEMVRLGLAWAFVKYSTTYVSVEAEARAKKVGVFQTDTMTPWDFRA
                     GTWRTAEEKAPAGCAIKGNVTANGRIYHMPWSPWYAKIRMDLDKGKRWFCSEEEAIAA
                     GWRPAMSH"
     gene            2782..3192
                     /locus_tag="PK264_03625"
     CDS             2782..3192
                     /locus_tag="PK264_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75017.1"
                     /translation="MLIDSIDHIVVNCRNVEATAAWYERALGLVREVYTSPAAPGPRI
                     ALKFGPHKFNLRQTGDASWETCRIDAPGSLDLCFLISGSLKPVMQRWQAAGIPVTVGP
                     VPRTGAQGRMTSVYTEDPDGNLVEIATYAADPLA"
     gene            complement(3200..5056)
                     /gene="mutL"
                     /locus_tag="PK264_03630"
     CDS             complement(3200..5056)
                     /gene="mutL"
                     /locus_tag="PK264_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRD75018.1"
                     /translation="MAIRQLSPEIVNRIAAGEVIERPASVVKELVENAIDAGASEIEV
                     VLAGGGLSLIRVTDDGSGMGRPDLALAVERHATSKLAHDDLFHIATLGFRGEALPSIG
                     SVAALSIQSNPRGERSGGAAILIDKGQKGAVGPAAVNPGTIVEVRDLFSATPARLKFM
                     KSERAENAAAAEVVRRLAMANPEIGFSLTAGERTSLRFPKAGPGPDGLLSRLGRIMGP
                     EFMRDALSVRGERDGIAIAGFAGLPTHNRTDAGQLYLFVNGRPVRDRLLLGALRAAYG
                     DLVPKGRQPVVALFITLAPAEVDVNVHPAKTEVRFRDAGAVRSAMIGSVRAALDSAGH
                     RSSARGGDTLVDVLANNATAAPPRVPQLAANTPAASPIAPRPAMRYPASRYSIARTMT
                     PGLADAMQAPLAGLEAISADTSAAVAEIAPAITARPLGAARAQLHSTYILAETATGVI
                     LIDQHAAHERLVYERLKAMLASGGIARQGLLVPEVVELAGEEIDALAERSDELAELGI
                     CLEPFGSGAVIVREVPALLGQADIRGLVADLARDLVSEGHSERLKDRLEAIASTMACH
                     GSVRAGRKLQPDEMNALLREMEATPHSGQCNHGRPTWIELSKTDLERLFKRR"
     gene            complement(5106..6452)
                     /locus_tag="PK264_03635"
     CDS             complement(5106..6452)
                     /locus_tag="PK264_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRD75019.1"
                     /translation="MSGAEAGAYTMSIEGCLAEAIGGHGLGRGTLASVLDELAPAFAD
                     LKASVGTGRMAFLDIVRARDDIAEARTALERLTRGARTLIFFGTGGSSLAGQTLAQLA
                     GWNIPGSAVESQRGRPRTRFYDNLDPVTLERSLATLDMATCRFVLISKSGGTPETLVQ
                     AAAAVSAIRRAGLGERIGEFILGITEPAAPGSANPLRKLLSSLGAPMLEHHPGIGGRF
                     SGLTNVGLVPAMARGLDPLAIRAGAADVIDALLAAPSPEACPPALGAAAIIGLARHRG
                     IRTLVMMPYADRLGRFAHWWVQLWAESLGKGGHGSTAHACLGPLDQHSQLQLFMDGPR
                     DHVVTVLRSPLAGTGPRVEADLAQAAGVDYLAGRTVGDLVAAQTSAIPEALRRAGRPV
                     RTFDLAALDEATLGALMMHMMLETILAARLLGVDPFGQPAVELAKDLTRQALRPKA"
     gene            complement(6576..>7249)
                     /locus_tag="PK264_03640"
     CDS             complement(6576..>7249)
                     /locus_tag="PK264_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD75020.1"
                     /translation="QTLLRMIQDAVANPAIPEFDEQQAIDELKQYLWAGTETTALTLA
                     WALYETARDPEAADRIRAEGEAVYGDREPTAADYSGLAYTRAVIQETMRLYPPIWGLV
                     RIAAEPDVIDGHEIRAGDRVALFAYGTHHSAKYWDEPERFMPERFMGDAAKKRKPYTY
                     IPFGGGKRSCIGGAMSQVENTLALSMLLRRFRPEYVGPNPAGIDATVTLAPRGGLKFR
                     IRERA"
     assembly_gap    7250..7294
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7295..>7602)
                     /locus_tag="PK264_03645"
     CDS             complement(<7295..>7602)
                     /locus_tag="PK264_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD75021.1"
                     /translation="PDIFAEYVPYFIQAIHRKMDLWSGYAKSGEEIEMVEQTWTLAAD
                     MICKALFDRDMPFNPHFVFKCVKTYTNILNHKSVRLKKVSGADFEITEEDTAKAMEVW
                     A"
     assembly_gap    7603..7636
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7637..7933)
                     /locus_tag="PK264_03650"
     CDS             complement(<7637..7933)
                     /locus_tag="PK264_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD75022.1"
                     /translation="MNAVPAHVERLGDQDSIWYKLSALSRGSPDNPMTTLMQLTERHG
                     SVIAVNLGTQRVVFLSEPEQFKRVLVTKVDKYIKYFDGLKPVFGKSMITHDGALW"
     assembly_gap    8121..8144
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8145..9343)
                     /gene="murA"
                     /locus_tag="PK264_03655"
     CDS             complement(<8145..9343)
                     /gene="murA"
                     /locus_tag="PK264_03655"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRD75023.1"
                     /translation="MDRIKIIGGQKLNGTIPISGAKNAALPLMIASLLTADRLTLKNV
                     PNLADVNLLARILRNHGVDLGVDGKRPGPSVHLGDTYHLTARDIVDTTAPYELVSRMR
                     ASFWVLGPLIARMGKARVSLPGGCAIGTRPVDLHLTGLKALGAEIEIDSGYVVAKAPR
                     GGLRGARIVFPKVSVGATHNVLLAAALSRGETVIENAAREPEIGDVATCLTRMGVEIA
                     GIGTSTLRIQGCDRLEGAVHTVLPDRIETGTFAMAVAAAGGDVTLEGTRKELLETALD
                     TLASTGTEVSETATGLRVVRNGKGIGPVTVETQPFPGFPTDLQAQLMALVTTASGTSV
                     IRETIFENRFMHVQELARLGADIRLHGDTATIRGVKGLKGAPVMATDLRASVSLVIAA
                     LVAEGETT"
     gene            9709..10674
                     /locus_tag="PK264_03660"
     CDS             9709..10674
                     /locus_tag="PK264_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD75024.1"
                     /translation="MSLSATPPKPWQPATYYPDPAIEALDPRFERYWIKLGAVERIAH
                     GFRWAEGPVWFGDGRYLLWSDIPNNRIMKWEEETGAVSVFRKPSNFANGNTRDRQGRL
                     VTCEHGRRVTRTEHDGSVTVLMDRFEGKRLNSPNDVVVARDGAIWFTDPPFGLLGNYE
                     GEKAEAELGQFVYRLDPATGRAEVVVDDLKGPNGLAFSPDESVLYVVESRAAPNRLLK
                     AYDVHASGKRLRNARTFIDAGPGTPDGFRCDVDGNLWCGWGMGRADLDGVRIFAPDGA
                     PIGHIHLPERCANVCFGGLKRNRLFMAASQSVYALYVQTQGAPGG"
     gene            complement(10699..11781)
                     /locus_tag="PK264_03665"
     CDS             complement(10699..11781)
                     /locus_tag="PK264_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HRD75025.1"
                     /translation="MIVRPKLGSALGLAAALLGLVAAACDAPSPRGRTSGLPNLGAAP
                     DQTTVSGISSGAYMAGQIQIAHSSRMLGAAIIAGGPFGCAESPYAVLVPGGSLMMNAT
                     RAINTCMKGLAGKPDAEPLVERVRRLAARGEIDPVATLALHRLYVFSGTRDEVVVPRV
                     AAAVPDLYERLGMPVERMKRVTTMAAGHAFVTENNGLSCSATGKPYIVGCGYDQAGDL
                     LAHLYGHMRPPSTARTGTLSVFEQSPFTRDLPFNAGMAISGAIYVPKDCADRAGCRIH
                     VAFHGCGQSRLAVGDAFIRETGFLRWSDTNRLIILFPQAAPSATNPQGCWDWWGYTGR
                     DFLTQKAPQIRAVMRMIDRLSEPAGR"
     gene            complement(11894..12802)
                     /locus_tag="PK264_03670"
     CDS             complement(11894..12802)
                     /locus_tag="PK264_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P44/Msp2 family outer membrane protein"
                     /protein_id="HRD75026.1"
                     /translation="MKVSYACLALGLFAATHVLPAEAGDFNTGAPGYRGPRAAGTMVP
                     APVPIPETFSWYLRADIGLGLPHQPSVSEVGRLYGRDPAGNLEALSTFGSSASWFQSD
                     FDTFLTGGVGVGYYITPRIRADVTLDARTEGEVSSSGSYMYVRHDHTAVPPAPIGEVY
                     GEVRERIAVRSTVGLFNAYYDLTERGRFTPYIGAGIGFAVHDVERSYTNTERVFDTTV
                     TPAVMTGTRFATARDKTHGVAFAGMLTTGVAWTLWQSTVLDLNYRYLYLGSTSTSVDV
                     YGAASKLTIGDQHEHQLRAGLRWNVW"
     gene            complement(12833..13810)
                     /locus_tag="PK264_03675"
     CDS             complement(12833..13810)
                     /locus_tag="PK264_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD75027.1"
                     /translation="MKAFCATLALGAALLTGSVAVANAADLYGAPGGSIKDATPVIPY
                     GSPMSWYIRGDIGWASHDNPVMVEDGIYDLVETKIGRNWTYGGGIGRYFSPSVRGDIT
                     VDVRTEADVRGRLADPGATAPGTRHFGLKSTVTLFNLYYDFDTRTRFTPYIGAGLGFA
                     HLKTTTGTITDTCGCGFTSTSIATGSDWHVAGALMAGFAFTVRDRVHLDAGYRFLYLG
                     EVATGPVTTTNALGTAMISKDPTVEDIHAHEFRLGLRMDIRSRTDTAPTPSTRRMLPA
                     DPFGRGSEDQRPDGRGRPGSLSISRSRKRTASSPPGETPRGAPVLSPDA"
     gene            complement(13956..14930)
                     /locus_tag="PK264_03680"
     CDS             complement(13956..14930)
                     /locus_tag="PK264_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75028.1"
                     /translation="MPLSHKPRNAVLRGLRSIAVAALLLISLAPLAAEDVRVPLGQPS
                     ADALRKVQRWVWFTGPPDVAALAGSPATMVIVDPEGVSAPSVQRLKRIGRRQGRLVLA
                     QIDIAMLTRAREPGSEITLEPAPGWLGPPACGMGWHRIRYWQVGWQDALFRDDTSYVM
                     QLLARGYDGLVLGGIEAYRPWQTERWQAAIEMAELVSELAIAIKTRRPSTLILLRNGE
                     EIGAHPDVLKAIDGIVRADLLYGARVKGAANSEATTARSVRNLAAARRAGLALFSLER
                     IADKTAAATADRLARGFGLLPGESAARGGQLVRPAVPPAFDVAGWPHC"
     gene            complement(15102..16478)
                     /locus_tag="PK264_03685"
     CDS             complement(15102..16478)
                     /locus_tag="PK264_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRD75029.1"
                     /translation="MSAAVDIADGQQTWKVFFASSIGTFFVGGLLALVAYDDGHARIG
                     IREGFLLTTLSWVVITAFAAMPFVGHGFSFTDSYFEAMSGLTTTGSTIIPNLDKQPRG
                     ILLWRAILCGFGGLGMIVIAIIMLPFLRVGGMQLFQTESSDKSEKVLPRAFQLVAATA
                     GVYLVLIVACAVVYAALGMTFFDAVCHALPTLATGGYSSHDDSFGFFKSPSIEWAGIV
                     FMALGAMPFVVFIKVFAGQPVTLWRDVQVRGFVTFLLAVSLMLGAWLSLTSGRPFLDA
                     FRSALFNVTSIVTTTGFASEDYSKWGPFAVGLFFLLTFVGGCSGSTSGGIKIYRLQVT
                     SILTRSHFLHLVSPNRIVMLAYNGRRLPEDVPFSVVAFLAIYMATVGLFTVVLSAFGL
                     DLVTALTGAATALSNVGPGLGEIIGPAGNFSSLPPLAKWVLSLAMMLGRLELFTVLVL
                     LRPEFWRN"
     gene            complement(16653..17138)
                     /locus_tag="PK264_03690"
     CDS             complement(16653..17138)
                     /locus_tag="PK264_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD75030.1"
                     /translation="MTIKVGDRLPETTFKIMGAEGPKDATTADVFANRKVALFAVPGA
                     YTPTCHKSPLPGFVRQYDEIKRKGVDVIACTAVNDIFVLSQWSKDSGADGKVLMLADG
                     SADFAKKIGLEIDLGSRGLGTRSKRYSMIVENGVVKALNVEDAPPSHDKSSAATLCTM
                     L"
     assembly_gap    17344..17435
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17436..18239)
                     /locus_tag="PK264_03695"
     CDS             complement(<17436..18239)
                     /locus_tag="PK264_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD75031.1"
                     /translation="MTGTLRLAHLSDIHMPVEPEIELADLNLKRMLGRIGWRRQRRFI
                     HSLDALERIVGDVAALRPDHIAVTGDLVNLGLGREIVAVRGLLARLGPPDRVSVIPGN
                     HDLYTRRRPGEAGIDVWSDYMGMVEARDGLESASDPRALHLFPYVRRLGTVALVGVNS
                     ARPTRPISAMGGVGESQRRRLALTLDQLAAEGLTRVMMIHHPPLPGQTTALRGLDDDK
                     EIEALLRRHGAELVLHGHNHRNMRAEVPGPRGPVPILAVPSASAGKVHGG"
     gene            complement(18236..18793)
                     /locus_tag="PK264_03700"
     CDS             complement(18236..18793)
                     /locus_tag="PK264_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRD75032.1"
                     /translation="MPADDKHSIDEPSSALAWSHAIDDIGPKGVRVERAASEAECAAI
                     ARQIGIRGCRSLAVKYQIRPMAGGAYFADGKLRATVTQSCVITLEPVEETIEEPFTVE
                     FRDASEIEAIEASEMEFDPDEPDDPEPIVAGRLMIGPPIIEHLILALDPYPRLPGATL
                     DQTSTGTEASRDNPFAALAKLKPRS"
     gene            complement(18780..19364)
                     /locus_tag="PK264_03705"
     CDS             complement(18780..19364)
                     /locus_tag="PK264_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HRD75033.1"
                     /translation="MLDWIRSRARKRRIAQDIYGSIVAQARSEAFYRGMGVPDTVMGR
                     LEMIILHTALLIERLQLEGEAGDRLAQDVAEAYVADMDDSIREIGIGDMGVPRRVKQA
                     AAALFERRRELAAVEAEYPGNEAALAAFLGRIVGNGAQVEPAGATALARYIGALRRAL
                     AENRLESVAEGRLDLSAAAALALVQDGSAPHARR"
     gene            19585..20037
                     /gene="bamE"
                     /locus_tag="PK264_03710"
     CDS             19585..20037
                     /gene="bamE"
                     /locus_tag="PK264_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HRD75034.1"
                     /translation="MQLRRLTLVRPSLALALALVLTACSSQITKHGHQFQETDIAQIQ
                     PGMSAEAVRMALGTPATTSSVGAGNAFYYISSTTSQTAFFKPTEVDRRVVAVYFTPVG
                     SVERVAHYGMKDGRVFDFVRRETPSHARDEGVLKSLFRNLGQKQLFGD"
     gene            complement(20196..>20995)
                     /locus_tag="PK264_03715"
     CDS             complement(20196..>20995)
                     /locus_tag="PK264_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HRD75035.1"
                     /translation="EPLSGLIHRYPDRVLLKLTLACPVYCRFCFRREMVGPGGPPPMV
                     GDRLVPALDYIRARPAIREVILTGGDPFMLAPRRIAEVTMGLGAIPHVASLRWHTRVP
                     VVDPERITDELVAALRHADRAVWIGIHTNHARELTPAARSAIRRLAAAGLALVGQTVL
                     LKGVNADAATLAELFGTLVGLGVKPYYLHHADLAPGTSHFRTTIAEGQALVKALRGRI
                     SGLALPTYVLDVPGGSGKVPLGPDHASNDGTGHVRTLDGERRSHPDA"
     assembly_gap    20996..21021
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21022..21240)
                     /locus_tag="PK264_03720"
     CDS             complement(<21022..21240)
                     /locus_tag="PK264_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRD75036.1"
                     /translation="MITNSRSTLRTITDLEREGLVPASAAQRLDRVAKRYAVAVTPAM
                     AALIEATNPADPIARQFIPDPRELDLASV"
     gene            complement(21278..21967)
                     /locus_tag="PK264_03725"
     CDS             complement(21278..21967)
                     /locus_tag="PK264_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD75037.1"
                     /translation="MELNADFAKRAVEHSAWRPWQPSPMAGVDRIMLDRIGGEVARAT
                     TIVRYAAGSRFSPHVHGGGEEFLVLDGVFQDEDGDYPAGNYVRNPPGSRHTPRSDLGC
                     TIFVKLWQFDPADRAVVRIDTARAASRELPGRPKVSVQPLFNDAREDVRIETWAPGAE
                     IAYDPVGGLELLVLEGAMAVCEDALGARDWLRLPIGMRLEALAGSEGCRFWVKEGHLR
                     FAKAVAPGTRG"
     gene            complement(22001..22288)
                     /locus_tag="PK264_03730"
     CDS             complement(22001..22288)
                     /locus_tag="PK264_03730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRD75038.1"
                     /translation="MSSPAPASEAGRRGRFADIKDMTFERALKELEAIVGRLERGDVE
                     LEESIAIHERGKALEEHCDKLLKAAEAKVEKLTFDAEGRPSGTTALDPERS"
     gene            complement(22326..23351)
                     /locus_tag="PK264_03735"
     CDS             complement(22326..23351)
                     /locus_tag="PK264_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HRD75039.1"
                     /translation="MATLLVTHPCFVEHDTGPGHPERPDRMRVLDPALAPERAEEPPF
                     RQKLARDNPVLRHWQALPREQFKPLVRREAPLIADVEEWIARVHPYQYVEAVKAARPG
                     PDDEAVRLDPDTVLSPTSWEPALRAVGAGLLAVDEVIAGRVQNAFCQVRPCGHHAETA
                     RAMGFCIFNNIAVAARYAQKKHGAERIAIVDFDVHHGNGTQDIFYADKSIYFASTHQM
                     PLYPGTGALSETGVGNIWNAPLRPGDGGSSFKEAFEARILPSLRNFAPDLVLISAGFD
                     AHEADPLANLRLLEDDFAWATGKLADIAHKHSGGRIVSMLEGGYDLAALANSVSLHVA
                     GLMDAGR"
     gene            complement(23414..24319)
                     /locus_tag="PK264_03740"
     CDS             complement(23414..24319)
                     /locus_tag="PK264_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD75040.1"
                     /translation="MADQSIRSTGDTESTPVARPRTSRYRVLGEGSSVTISVVTIALC
                     FILWWLATRQGLKYPAFSFENGFHWGETPLLKPLFLPKPESIWLAFRQAMEGELDNST
                     LVGHFLASLGRVGSAFLLAVALGIPVGIAMGVSRIARGVFDPPLEFYRPLPPLAYLPL
                     MIIWFGIGEKNNIILLFLAIFAPVALAARAGVRSVSQEQIQAALSMGASRMQVVRHVI
                     VPGALPEILVGLRIGMGVGWTTLVAAEMVAADAGLGKMVHNASNFLRTDVVVLGILAI
                     GFVAYAFELAMRYAERLLVPWKGRV"
     gene            24462..24746
                     /locus_tag="PK264_03745"
     CDS             24462..24746
                     /locus_tag="PK264_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75041.1"
                     /translation="MADKTTLVLPGRKFTVSKVLKRTLQIAVAVSLTSLLALVVVIAN
                     GMLIGRGATLQAGVATWLSFIKRPDIQTTMLLTAVVSVLLVYWQRDHEKR"
     gene            complement(24749..25507)
                     /locus_tag="PK264_03750"
     CDS             complement(24749..25507)
                     /locus_tag="PK264_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD75042.1"
                     /translation="MKDLAVRYATDTGVTEALSRVNLDMTAGDFLVAIGASGCGKTTL
                     LSVIAGFLAPSDGEVLLDRHPVAGPGADRGVVFQRHALMPWLNVIDNVAFGLKMRGIP
                     REERHAIAREKLALVGLDEFANKMIYELSGGMQQRVGIARALASDPEMLLMDEPLGAL
                     DALTREQVQELILDVWHRTGKMIFFITHSVEEAIFLATRLIVMTPRPGKIEKSYTLDF
                     GRRFIESRDARAVKSHPEFIRVREQVLADIQRRV"
     gene            complement(25641..26657)
                     /gene="tauA"
                     /locus_tag="PK264_03755"
     CDS             complement(25641..26657)
                     /gene="tauA"
                     /locus_tag="PK264_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD75043.1"
                     /translation="MRPLRMVSAGMAAAIGLALGATAAMAQPKKEVTFAHQDMIIPYR
                     VLMAAGEIEKKTGYKISWKMFGGGGDVIKAMASGEVPIGEVGSSPATAAASQGIDIQV
                     IWILDDIGNAEQLVASKKSGVTTLAGLKGKKVATPFVSTAHYQLLFALKDAGVNAKEV
                     NILNMRPPEIAAAWERGDIDATFIWDPVLGNIKKGGTVLISAGEIAKKGAPTFDALIV
                     NRAWAAQHKEFVTTLVKMIAAKDAEFRANKAKWVAGSAEVAAIAKIVGAKPENVPAVL
                     NDYIFPTAEVQASKAWLGGGAEGGVARAMLDTARFLKEQGRITDVAADYAKFVTPEFA
                     AAAK"
     gene            complement(26847..27296)
                     /locus_tag="PK264_03760"
     CDS             complement(26847..27296)
                     /locus_tag="PK264_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD75044.1"
                     /translation="MTTRVIAVLVAAAISLIGPAGAADNVTSTTAPDLTAVRALIKAK
                     EFAKARDELNRIADGTQHADVYNLLGFTNRKLGDYPTALTFYKKALDFDPEHKSAHEY
                     LGELYVETGDLEKARGHLAILVRLCPTGCEEREDLETAIAAAAPKIK"
     gene            complement(27359..>27972)
                     /locus_tag="PK264_03765"
     CDS             complement(27359..>27972)
                     /locus_tag="PK264_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRD75045.1"
                     /translation="ATGTLPAISGTAVMAEPVLFRPAFSIGAMIELVIPLAIMVLVVQ
                     NGQGAAVLKVAGHDTPINACSAACGVFSLAAGLVGTVSTCLTGPTNAIITSSGARESH
                     FAAAVMVGAIALGVGIFSPIVTRVLIGAPPGFLATLAGLAMLRVLQTAFVAAFSGRFQ
                     LGGLVTFLVTVAGIPVLNIGAAFWGLLLGLVTSWLLERSDFTG"
BASE COUNT         4591 a         9123 c         9364 g         4526 t
ORIGIN      
        1 cgggcatgtc ctcgtcgcca ccttcgggcc cgatggcccc gagcgctgca gcgggctgga
       61 tgtcctccgg tacggggcgg aggagatcca cgccgagttc ggccccgcct tccgcaaggt
      121 cgggagcgcc accgaggagc acacgacgcc cgggggatcg gttcagcagt tcgtctattg
      181 ctactgccgg ctgcctggcg gcggggcgag gcccgctgcc ggaaacgcgt cggcgagata
      241 ggcgcagatt gcggcgcact cggtcaccac atgggagccg tgcgcgaggg ccggcacctt
      301 gcccatggga ttgatggcga gataagcagt cgatttcatc ttcgggccgt agtcgatcac
      361 ctcggtacga tagggcgcgc cgacctcctc gagcatccag cgcacgatgc gaccgcgcga
      421 catcggatgc gtgtagaaga caagctcatc gctcatgtca tgctccatca tctgagccgc
      481 ctcacgccga agcgatagca cgccggccca cgatcgtgga aactggtggc gtcgggagag
      541 tgccgaggca ctctcggtcc ggcgcgcggc gcggctcaag gaaagacggc gaacttggca
      601 gatctggcga ccgtccggac gagctccgac aggcatgccg accggcgcgg cctctggatc
      661 gcggccggcc tcgcgctcgg catggccgtc gtcaacagct ttgcgcgctt tgcctatgcg
      721 ctggtgcttc ccgccatgcg ctccgacctc ggctggagct acacggatgc gggcgccctc
      781 aacacggcga atgcgatcgg ctacctgatc ggggcggcgg cggcgttccg ctccgtccga
      841 cgcctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaagcgt ctttccggga cggggcgcgg
     1021 cgtcggcgcg tgccatcgcc atctacttcg gcggcggcgg attcggcctg ctcatgtccg
     1081 cgatcaccat cccgccgctg ttggcgatgg ccggcgaccg ggcgtggccc aaggcttggc
     1141 tgctgctcgg ggcgatgtcg gcggcagcga gtatcgcggc gacgctcgcc gcacgccgcg
     1201 tcgcagcgcc tgcaccggtg gctgcgccgg tcgcctgggc caagctgccg ctgctcgcga
     1261 ctttcctcgc ctatgcctgc ttcgcgttcg gctacaccgc ctatatgacc ttcatcgtcg
     1321 ccttcatgcg cgataacggc gcaggctcgg tcgagatcgg cctcatgtgg gcgatgctcg
     1381 gcctggcgac actcgctgga cccaggatct ggtccgggcc gatgtcgagc tggccgccgg
     1441 ggttcgcgct cgcaggcgtg ctcgcgacga tcggcatcgg agcaacactc cccgttatcg
     1501 tcgcgacccc tgccgtcatg atcgtgtctg ccgccctgtt cggcgccttc ttcatgacgc
     1561 cggcgagcgt gactgccatc gtgcggacgg ccctgccgcc ggtcgtgtgg ggcgaagcgg
     1621 tggcggcgtt cacgctgatg ttcgccctcc tgcagtgctt cggtccgatc ctgacggggc
     1681 tgctcgccga cttgaccgga gacctcgagg acgggctctc agtgtccgcc gccgtgctca
     1741 tggctggagc agcgctcgcg ctcctgcagg gcctcgaggc aaggccgcgc cgataggacc
     1801 ggccactacg gcgatacaac cttaacgagt tgacacctga gtgtggcaga gtggccgcat
     1861 tgtgtttcat ttttgttcca atgcttgatc ggccgtggat cggtcgcccc atcgcctaga
     1921 ttccgacatt cgaatcagcg atgttcacgt tatggttgaa tcggtccgtc agtatgcaat
     1981 ccgacagtca gtggcgttcg cgctggctgc cgcactcgca ctggttgtat ggtcgctcgc
     2041 gcccggccaa cccgaggcgc gctcggcggc cgatatcgct ggcatggccc gcgtcctcga
     2101 tggcgatacg gtcgaaatct cgggcgtgcg catccgtctc gagggcatag acgcgcccga
     2161 ggccgcgcaa cgctgtacgc atcgcgtgat cggcacctgg gcctgcggcg ctgccgcgac
     2221 caacgagctc gtccgcctcg tcgacggcaa gccgctcacg tgcacgccgc tcggcaccga
     2281 taagtatggc cgcacgctcg cgacctgcca tctcggcacg ctcgacgtca atgcggagat
     2341 ggtgcgcctc gggctcgcct gggccttcgt caaatactcg accacctacg tctcggtcga
     2401 ggccgaggca cgcgccaaga aggtcggcgt gttccagacg gacaccatga cgccgtggga
     2461 tttccgcgcc ggtacctggc ggacggccga ggagaaggca cccgcgggct gcgccatcaa
     2521 gggcaacgtg accgccaacg gccgcatcta tcacatgccc tggagcccct ggtacgccaa
     2581 gatccgcatg gacctcgaca aaggcaagcg ctggttctgc tccgaggagg aggcgatcgc
     2641 cgccggctgg cgtcccgcca tgtcgcactg agtggtccgc ggccaagacg gccgcggccg
     2701 cgaactggtg atccggggcg cgctcgacta cgattgcgtg ccggataaga acggctcgac
     2761 cgatctcgag cgaggcgcgc catgctcatc gacagcatcg accatatcgt cgtcaactgc
     2821 aggaacgtcg aggcgacggc cgcctggtat gagcgagcgc tcggcctcgt ccgcgaggtc
     2881 tacacgagtc cggcagcgcc cggaccgcgg atcgcgctca agttcggacc gcacaagttc
     2941 aatctccgcc agaccggcga cgcgagctgg gagacctgcc gcatcgatgc gccgggctcg
     3001 ctcgatctct gctttctgat cagtggcagc ctgaagcccg tcatgcagcg ctggcaggcg
     3061 gccggcatcc cggtcacggt cggccccgtc ccgcgcaccg gcgcacaggg ccgcatgacc
     3121 tcggtctaca ccgaggaccc ggacggcaac ctcgtcgaga ttgcaaccta cgccgccgat
     3181 ccgctcgcct gacgcggcct cagcgccgct tgaagagccg ctcgaggtcg gtcttggaga
     3241 gttcaatcca ggtcggccgg ccgtgattgc actggcccga gtgcggtgtc gcctccatct
     3301 cgcgaaggag cgcgttcatc tcgtcaggct gcagcttgcg gccggcccgc acgctgccat
     3361 ggcaggccat ggtcgacgcg atcgcctcga gccggtcctt caagcgctcc gaatggccct
     3421 cgctcacgag atcgcgggcg agatccgcga ccagcccgcg gatgtccgcc tggccgagga
     3481 gcgccggcac ctcacgaacg atcaccgcgc cggaaccgaa cggctcgagg cagatgccga
     3541 gctcggcaag ctcgtccgag cgctcggcga gcgcatcgat ctcctcgccc gcgagctcga
     3601 cgacctcggg aacgagcagg ccctgccgcg cgataccgcc gctcgccagc atcgccttga
     3661 gccgctcata aacgaggcgc tcgtgtgctg cgtgctggtc gatcaggata acgcccgttg
     3721 ctgtctcggc gaggatgtag gtgctgtgga gctgggcgcg ggcggcgccg agtgggcgag
     3781 cggtgatggc cggcgcaatc tcggcaacgg cggccgacgt atcggccgag atcgcctcga
     3841 gcccggcgag cggcgcttgc atcgcatcgg ccaggcccgg cgtcatggtg cgggcgatcg
     3901 agtagcgtga cgcggggtag cgcatcgccg gcctaggtgc gatcggagat gctgcaggag
     3961 tgttcgccgc aagctgtggc acccgtggcg gggctgcggt cgcgttgttg gccaagacgt
     4021 cgacaagggt gtcgccgccg cgcgcgctcg agcgatggcc ggccgaatcg agggctgcgc
     4081 gtacagagcc gatcattgcg ctccggacgg cgcccgcatc gcggaagcgc acctcggtct
     4141 tggccggatg cacgttcacg tcgacctcgg ccggcgccag cgtgatgaag agcgcgacca
     4201 ccggctgccg ccccttcggc acgagatcgc cgtaggccgc gcggagcgcg ccgagcagca
     4261 ggcggtcgcg caccggccgc ccattgacga acaggtagag ctggccggcg tcggtccggt
     4321 tgtgcgtcgg cagacccgcg aagccggcga tggcgatgcc gtcgcgctcc ccgcggaccg
     4381 aaagtgcatc gcgcatgaac tcggggccca tgatgcggcc gagccgcgag aggagcccgt
     4441 ccggccctgg acccgccttc ggaaagcgca agctcgtgcg ctcgccggcg gtcagcgaga
     4501 agccgatctc ggggttcgcc atcgcaaggc ggcggacgac ctcggcggcg gcggcattct
     4561 cggcgcgctc ggacttcatg aatttgagcc gcgccggcgt cgccgagaag agatcgcgca
     4621 cctcgacgat ggtgccggga tttacggccg cgggaccgac cgcgcctttc tgccccttgt
     4681 cgatgaggat cgcggcgccg cccgagcgct cgccgcgcgg gttcgactgg atcgagagcg
     4741 cggccaccga accgatcgag ggcagcgcct cgccgcgaaa gccgagtgtc gcgatgtgaa
     4801 aaaggtcgtc gtgggcgagc ttcgacgtcg cgtggcgttc taccgccagc gcgagatcgg
     4861 gccggcccat gccggagccg tcgtcggtca cgcggatgag cgagagcccg ccacccgcca
     4921 gcaccacctc gatctcgctc gctccagcgt cgatggcgtt ctcgacgagc tccttcacga
     4981 cgctcgccgg ccgctcgatc acctcgccgg cggcgatgcg attgacgatt tccggcgaga
     5041 gctgtcgaat ggccacgccc tggctccgct gccaactatc cagcgctcgg cccgcgacgc
     5101 gcgcattacg ccttggggcg cagcgcctgc cgggtcagat ccttcgcgag ctcgacggcc
     5161 ggctgcccga agggatcgac gccgagaagt cttgccgcga gaatcgtctc gagcatcatg
     5221 tgcatcatca gagcgccaag cgtcgcttcg tcaagtgcgg cgagatcgaa tgtgcgaaca
     5281 ggacggcccg cgcgccgcag agcctcgggg atggccgagg tctgcgcggc gacgagatca
     5341 ccgaccgtcc ggccggcgag atagtcgacg ccggccgcct gtgcgaggtc ggcctcgacc
     5401 cgcggccccg ttccagcgag aggactacgc agcactgtca ccacgtgatc gcgcgggcca
     5461 tccatgaaga gctgcagctg gctgtgctgg tcgagcgggc ctaagcaggc gtgcgccgtg
     5521 ctgccatggc cgcccttgcc gaggctctcg gcccacagct gcacccacca gtgcgcaaac
     5581 cggccgagcc ggtcggcata gggcatcatg acgagggtgc ggatgccgcg atggcgtgcg
     5641 aggccgatga tggcggcagc accgagcgcg ggcggacacg cttcgggaga cggcgccgcc
     5701 agcagcgcgt cgatgacgtc tgcggcgccg gcgcggatgg cgagcgggtc gaggccccgc
     5761 gccatggccg gaacgaggcc gacgttcgtc aggcccgaga agcgtccgcc gataccggga
     5821 tgatgctcga gcatgggcgc gccgagcgag gacagaagtt tgcgcagagg atttgcggaa
     5881 ccgggagccg ccggctccgt gatgccgaga ataaactcgc cgatccgctc gccgagcccg
     5941 gcacgccgga ttgcgctcac cgctgccgcc gcctggacaa gcgtctcagg cgtgccgcct
     6001 gacttcgaga tgagcacgaa gcggcacgtc gccatgtcga gggtggcgag cgagcgttcg
     6061 agcgtcacgg ggtccaggtt gtcgtagaag cgcgtccgcg ggcgcccgcg ctggctctcc
     6121 acggcactcc ccgggatgtt ccagccggcg agctgcgcga gtgtctgccc cgcgagacta
     6181 gagccgccgg tgccgaagaa tatgagcgtg cgggcaccgc gcgtgagccg ttcgagggcg
     6241 gtgcgtgcct cggcgatgtc gtcacgcgcg cgcacgatgt cgaggaacgc catgcgcccc
     6301 gtgccgacgc ttgccttcag gtcagcgaag gccggtgcaa gctcatcaag cacgctcgcg
     6361 agggtgccgc gcccgagccc gtgcccgccg atcgcctcgg caaggcagcc ctcgatcgac
     6421 atcgtatagg cgccagcttc ggctccagac atggctcgag agcctcccgt gtgaacggcc
     6481 gccgccgccg acgtcggatc attccggcgc ggccgagaga gaccacctcg gcgccggcgc
     6541 gtcaacacgc ctctccgaac cgctggatgc ccgtctcacg cccgctcgcg gatgcgaaac
     6601 ttgaggccgc cgcgcggcgc gagcgtcacc gtcgcgtcga tgccggccgg gttcggcccc
     6661 acgtactcgg ggcggaagcg gcgcagcaac atggagagtg cgagtgtgtt ctcaacctgg
     6721 ctcatggcgc cgccgatgca agagcgcttg ccgccaccga acgggatgta ggtgtagggc
     6781 ttgcgcttct tggcagcgtc ccccatgaag cgctccggca tgaagcgctc cggctcgtcc
     6841 cagtacttgg ccgagtggtg cgtgccatag gcgaagaggg cgacacgatc gccggccctg
     6901 atctcgtgcc cgtcgatgac atctggctcg gcggcaatgc gtacgagccc ccagatcggc
     6961 ggatagagcc gcatcgtctc ctggatcacg gcgcgcgtgt aggcgaggcc cgagtagtcg
     7021 gcggccgtcg gctcgcggtc gccatagacg gcctcgccct ccgcgcggat gcgatcggcc
     7081 gcctcggggt cgcgtgcagt ctcgtaaagt gcccaggcga gtgtcagcgc ggtcgtctcg
     7141 gttccggccc aaaggtactg cttcagctcg tcgatcgcct gctgctcgtc gaactcgggg
     7201 atcgcgggat tggcgaccgc gtcctggatc atgcgcagca gcgtttgctn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcccac acctccatgg ccttggcggt
     7321 atcctcctcg gtgatctcga agtcggcgcc cgagaccttc ttcaggcgga cggacttgtg
     7381 attgaggatg ttcgtatagg ttttcacgca cttgaagacg aagtgcggat tgaacggcat
     7441 gtcgcgatcg aacaaggcct tgcagatcat gtcggcggcg agcgtccagg tctgctcgac
     7501 catctcgatc tcttcgcccg acttcgcata gccggaccag aggtccattt tcctgtggat
     7561 ggcctggatg aaatagggaa catactcggc gaagatgtcc ggnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnccag agcgcgccat cgtgcgtgat catcgacttg ccgaacaccg
     7681 gcttcaggcc atcgaagtac ttgatgtact tgtcgacctt cgtgacgagc acccgcttga
     7741 actgctcggg ctccgacagg aacacgacgc gctgcgtgcc gagattgacc gcgatcacgc
     7801 tgccgtgccg ctcggtcagc tgcatgagcg tggtcatggg attgtcggga ctgccccgcg
     7861 acaacgcaga cagcttgtac cagatgctgt cctggtcgcc gagccgttcg acgtgggctg
     7921 gaactgcgtt catcggcacc ctcggttgtc gttcagggct cgcgtctcgc acagatggcg
     7981 cgagcacggc gcctgctaac acccgacgcg agctcaagtc cagcaagtgc ccgcgccagc
     8041 tttaactctt gcgctacccc gatatgcgtt cgatgcgtgc cccacagcgc ccgagcttct
     8101 cttcgaggcg ctcgaagccg nnnnnnnnnn nnnnnnnnnn nnnngtcgtc tccccctcgg
     8161 ccacgagcgc ggcgatgacg agcgagacgg aggcgcgcag gtcggtcgcc atcaccggcg
     8221 cgcccttcag gcccttcacg ccgcggatcg tcgcggtatc gccatgcagg cggatgtcgg
     8281 cgccgaggcg cgcgagctcc tgcacgtgca tgaagcggtt ttcgaagatt gtctcgcgga
     8341 tgacgctcgt cccgctcgcc gtggtgacca gcgccatgag ttgcgcctgc agatcagtcg
     8401 gaaagccggg aaatggctgt gtctccacgg tcacgggacc gatgcccttg ccgttgcgca
     8461 cgaccctgag gcccgtcgcg gtctcgctga cctcggtgcc cgtcgaggcg agcgtgtcga
     8521 gcgccgtctc gagcaattcc ttgcgcgtcc cctcaagggt gacatcacca ccggcagctg
     8581 ccactgccat ggcgaacgtg ccggtctcga tgcgatccgg cagcacggtg tgcaccgcgc
     8641 cttcgagccg gtcgcagccc tgaattcgca gcgttgacgt gccgatgccg gcgatctcga
     8701 cgcccatgcg ggtcagacac gtcgctacgt cgccgatctc gggctcgcgc gcggcattct
     8761 cgatgaccgt ctcaccccgt gacagcgcgg cggcgagcag cacgttgtgg gtcgcgccca
     8821 cggacacttt cgggaatacg attcgcgcgc ccctgaggcc gccgcgtggc gccttggcga
     8881 cgacgtagcc gctgtcgatc tcgatctcgg cgccgagtgc cttcagcccg gtgaggtgga
     8941 gatcgacggg ccgcgtgccg atggcgcaac cgcccggcag cgacacacgc gccttcccca
     9001 tgcgcgcgat gagcggcccg agcacccaga agctcgcacg catgcggctc acgagttcgt
     9061 atggtgcggt cgtgtcgacg atatcgcgcg cggtcagatg atacgtgtcg ccgaggtgca
     9121 cgctcggtcc cggacgcttg ccgtcgacgc cgaggtccac gccgtggttg cgcaggatgc
     9181 gcgcgagcag gttgacgtca gcgagattgg gcacgttctt cagggtgagg cggtcggcgg
     9241 tcagcaggct ggcgatcatt agggggagag ccgcgttttt cgcaccggag atcggtatgg
     9301 tgccgttgag cttctggccc ccgatgatct tgatgcgatc catgcgctta aggctccctc
     9361 gccccatcag tgtcttcaga aactacagcg ggcagcgacc agcgacctcg cgccggcagg
     9421 catcgctgtt tccccaagca agcgcgcggc ggacgagcct caccacgcct tcaaccgaac
     9481 aagcactccc atcaatacgc atgtcgagca cttcctgatg acgggccgct cctgatagag
     9541 cggatgcgac acggcattcc atgaccctag tcaagcgtgg ctctgcaaca accagccatt
     9601 gcgacgaacg cttgtcgcag gctcgatctt gcgatgcaat aggggctcgg gtcgcagtgc
     9661 cgcacgcctg tggctccctt acaaccatag cgccgatcga ggttccccat gtccctgtct
     9721 gcaacaccgc ccaagccctg gcagccggcg acctactacc ccgatcccgc catcgaggca
     9781 ctcgacccgc gcttcgaaag atattggatc aagctcggtg cggtggagag gatcgcacat
     9841 ggctttcgct gggcggaagg tcccgtctgg ttcggcgacg ggcgctatct cctttggagc
     9901 gatatcccca acaatcgcat catgaagtgg gaggaggaga caggcgcggt cagcgtcttc
     9961 cgcaagccct cgaactttgc caacggcaat acgcgcgacc gccaaggccg cctcgtcacc
    10021 tgcgagcacg gccgtcgcgt gacccgtacg gagcacgatg gaagcgttac ggtgctcatg
    10081 gaccgcttcg aggggaaacg cctcaattcg ccaaacgacg ttgtcgtggc gcgcgatggc
    10141 gccatctggt tcaccgaccc gccgttcggc ctcctcggca actacgaggg cgagaaggcc
    10201 gaggcggagc ttggccagtt cgtctatcgg ctggaccccg cgaccggccg cgccgaggtc
    10261 gtggtcgacg acctgaaggg cccgaacggg ctcgccttct cgcccgacga gagcgtgctc
    10321 tacgtggtcg agagccgcgc ggcgccgaac cgactgctca aggcctacga cgtgcacgcg
    10381 agcggcaagc ggctccgcaa tgcgcgcacc ttcatcgacg cgggtcccgg cacgccggac
    10441 ggcttccgct gcgatgtcga cggaaacctc tggtgtggct ggggaatggg ccgtgctgac
    10501 ctcgacggcg tgcgcatctt cgcgcccgac ggcgcaccga tcgggcatat ccacctcccc
    10561 gagcgatgcg ccaacgtctg cttcggcggc ctcaagcgca accggctgtt catggcggcg
    10621 agccagtcgg tgtatgccct ctacgtgcag acgcagggcg ctcccggcgg ctgacatcgg
    10681 cgagccccaa aggacccatc agcggccggc gggctcgctc agccggtcga tcatgcgcat
    10741 gaccgccctg atctgcggcg ccttctgcgt gaggaaatcc cggcccgtat agccccacca
    10801 gtcccagcaa ccctgcggat tggtggccga tggcgctgcc tgcgggaaga ggatgatcag
    10861 ccggttggta tcggaccagc gcaggaatcc ggtctcgcgg atgaaggcgt cgccgacggc
    10921 gagccgcgac tggccgcagc catggaaggc aacgtggatg cggcagccag cgcgatcggc
    10981 gcagtccttc ggcacataga tcgcgccgga gatcgccatg cccgcattga acggcaggtc
    11041 gcgggtgaaa ggcgattgct cgaagacact cagtgttccg gtccgcgccg tcgagggcgg
    11101 tcgcatgtgc ccgtagagat gggcgaggag atcgcccgcc tgatcatagc cacagccgac
    11161 gatgtaaggc ttgcccgtcg cgctgcagga gaggccgttg ttctcggtga cgaaggcgtg
    11221 gccggcggcc atggtcgtca cccgcttcat gcgctcgacg ggcatgccga ggcgctcgta
    11281 gaggtccggc actgccgccg cgacgcgcgg cacgacgacc tcgtcgcgcg tcccagagaa
    11341 gacgtagagg cgatgcagcg ccaaggtcgc gaccgggtcg atctcgccgc gtgccgcgag
    11401 tcgccgcaca cgctcgacga gcggctccgc gtcgggcttc cccgcgagcc ccttcatgca
    11461 agtgttgatt gctcgcgtgg cgttcatcat caggctgccg cccggcacca ggacggcata
    11521 ggggctctcg gcgcagccga aggggccgcc cgcgatgatg gccgcaccga gcatgcggct
    11581 cgagtgcgcg atctggatct ggcctgccat ataggcgccg gacgaaatgc cggagaccgt
    11641 cgtctgatca ggggccgcgc cgagatttgg cagcccggac gtgcgtccgc gcgggctcgg
    11701 cgcatcgcag gcagccgcga cgagaccaag cagcgccgcc gcgaggccga gcgcactgcc
    11761 gagcttggga cgcacgatca tgcccttagc cctcacttcg acttcgttcg acgcccctcg
    11821 agaccttcga ccacgcagcg aagggccgcc gatcctctcg gcaacccctc aattctgcct
    11881 tgcggagtgc tccttaccac acgttccagc gcaggccggc gcgaagttga tgctcgtgct
    11941 ggtcgccgat ggtcagcttg ctcgccgcgc catagacgtc gaccgaggtg ctcgtgctgc
    12001 cgagatagag gtagcggtag ttgagatcga gcactgtact ctgccagagc gtccaggcga
    12061 cgcccgtcgt cagcatgccg gcgaaggcga cgccgtgggt cttgtcgcgg gcggtggcaa
    12121 agcgcgtgcc cgtcatgacg gcaggagtaa cggtcgtatc gaagacgcgc tcggtgttcg
    12181 tgtaggagcg ctcgacatca tgaacggcga agccgatgcc tgcaccgatg tagggcgtga
    12241 agcggccgcg ctcggtgagg tcgtaatagg cgttgaacag gccgacggtc gaacgcacgg
    12301 caattctctc tcgcacctcg ccatagactt cgccgatcgg agcgggcggc acggcggtat
    12361 ggtcgtggcg cacgtacatg tacgagccac tcgagctcac ctctccctcg gtgcgcgcat
    12421 cgagtgtcac gtcggcacgg atgcgcgggg tgatgtagta gccgacaccg acgccaccgg
    12481 tcaggaaggt atcgaaatcg ctctgaaacc aggaggccga gctgccgaat gtcgacaagg
    12541 cctcgagatt tccggctgga tcacgcccat agaggcgccc gacctcggaa accgacggct
    12601 ggtgcgggag gccgaggccg atatcggcgc gcagatacca ggagaaggtc tccggaatgg
    12661 ggaccggggc cggcaccatc gttccggcgg cgcgaggtcc acgatagccg ggagcgcccg
    12721 tgttaaaatc gccggcttcg gccgggagga catgcgtggc agcgaagagg ccgagcgcaa
    12781 ggcaggcgta cgagactttc atggagatcg gtccctgagg tgacggcaaa tttcatgcgt
    12841 cgggggaaag aaccggggct cctcgaggcg tctcacctgg tggcgaactc gccgtacgct
    12901 tacgggacct ggagatcgaa agagaacccg gacggccgcg accatccggt cgctggtctt
    12961 cactcccccg gccgaacgga tcggccggga gcatgcgtct tgtcgacggc gttggcgccg
    13021 tatcggttct ggatcggata tccatgcgca ggccgaggcg gaactcgtgc gcgtggatgt
    13081 cctcgaccgt cggatccttt gagatcatcg cggtgccgag cgcgttggtc gtcgtcacgg
    13141 gaccggttgc gacctcaccg aggtagagga agcggtagcc cgcatcgagg tggacgcgat
    13201 cgcggaccgt gaatgcgaag cccgccatca gcgcacctgc gacgtgccag tccgagcccg
    13261 tcgcgatgga ggtgctcgtg aagccgcaac cgcaggtgtc ggtgatcgtg cccgtcgtcg
    13321 tcttcagatg cgcaaagccg aggcccgcgc cgatgtaggg cgtgaagcgg gtgcgcgtat
    13381 caaagtcgta gtagagattg aagagcgtga ccgtgctctt cagtccgaag tggcgcgtgc
    13441 cgggcgccgt ggcacctgga tcggcaaggc ggccgcgcac gtcggcctcg gtgcgcacat
    13501 cgacggtgat gtcgccgcgc acgctcggcg agaagtagcg gccgatgccg ccgccgtagg
    13561 tccagttgcg gccgatctta gtctcgacga gatcgtagat gccgtcctcg accatcaccg
    13621 gattgtcgtg cgatgcccaa ccgatatcac cgcggatgta ccacgacatc gggctgccgt
    13681 acggaatgac gggcgtcgca tccttgatgc tgccgccggg cgcgccgtag agatccgcgg
    13741 cgtttgccac cgcaacactg cctgtcagca gggccgcgcc gagggcaagg gtcgcgcaaa
    13801 aagctttcat gaccctagtt gccgaaaccg gcctcgaagc atgccggcaa gcatccgagc
    13861 ccgcacagaa cgctgaacac gcgactgctc gtaggccgac tatcggcgac aacatttaag
    13921 aagggcttaa gcggtggccg gttccgggac gctgctcagc aatgcggcca gccggcgacg
    13981 tcgaatgcgg gcggcaccgc cggccgcaca agctggccgc cacgcgccgc gctctcgcct
    14041 gggaggaggc cgaaaccacg cgcgagccgg tcggcagtag cggccgctgt cttgtccgcg
    14101 atcctctcga gactgaagag cgccagtccg gcgcgccgtg ctgcggcgag gttgcggaca
    14161 ctgcgcgccg tcgtcgcctc gctgttggcg gcgcccttca ccctcgcgcc gtagaggagg
    14221 tcggccctga cgatgccgtc gatggccttg aggacatccg ggtgtgcgcc gatctcctcg
    14281 ccgttgcgca ggaggatcag cgtgctcggc cgcctggtct tgatggcgat ggcgagctcc
    14341 gagacgagtt cggccatctc gatcgcggct tgccagcgct ccgtctgcca ggggcggtac
    14401 gcctcgatgc cgccgagcac gaggccatcg tagccccggg caagaagctg catgacatac
    14461 gaggtgtcgt cccggaacag cgcatcctgc cagccgacct gccagtagcg gatacggtgc
    14521 cagcccatgc cgcatgccgg cggcccgagc caccccggtg ccggctcgag cgtgatctcc
    14581 gagcccggct cgcgcgccct cgtgagcatg gcgatatcga tctgggcgag gacgagccgc
    14641 ccttggcgcc gtccgatacg cttgagccgc tggacactcg gggccgagac accctcgggg
    14701 tcgacgatca ccatcgtcgc ggggctgccg gcgagagctg caacatcagg cggccccgtg
    14761 aaccataccc agcgctgcac cttgcgcaat gcatcggccg agggctgccc gagcggcacg
    14821 cgcacatcct cggcggcgag cggcgcgagg ctgatgagca agagcgcagc gaccgcgatc
    14881 gagcgcaggc cgcgaagtac ggcgttccgg ggcttatggg acagcggcac tgggatgtcg
    14941 gatctcgcgc ttggcggcct gctctggcga gcatgtcggc tcacgcgctg ctggtcaacc
    15001 tgccacgaca ggtcgcaggt gctcccagtc gatccggatt gtcgcacgca tacatgagct
    15061 tatcttggaa tgctgcggca agggtgcgac tgggtccgcc atcagttgcg ccagaactcc
    15121 ggcctgagca gcacgagcac agtgaagagc tcgagccggc cgagcatcat ggcgagcgag
    15181 agcacccact tggcgagcgg cggcagcgat gagaaattgc cggcagggcc gatgatttcg
    15241 ccgagccccg ggccgacgtt gctgagcgcg gttgccgcac ccgtaagcgc cgtgacgaga
    15301 tcgaggccga aggccgacag caccacggtg aacagtccga cggttgccat gtaaatggcg
    15361 aggaaagcga ctaccgagaa tggcacgtcc tccggcaggc gccggccatt gtatgcgagc
    15421 atcacgatcc ggttcgggct gacgagatgc aggaaatggc tgcgcgtcag gatcgaggtc
    15481 acctgcagcc ggtagatctt gatgccgccc gatgtcgagc cggagcagcc gccgacgaac
    15541 gtcagcagga agaagaggcc gaccgcaaac ggaccccact tcgaatagtc ctcagaggca
    15601 aagccggttg tggtgacgat cgacgtcaca ttgaagaggg ctgagcggaa ggcgtcgagg
    15661 aacggccggc cgctggtcag cgacagccag gcgccgagca tcaggctgac ggcaagcagg
    15721 aaggtcacga agccgcgcac ctgcacatcg cgccagagcg tcaccggctg tccggcgaac
    15781 accttgatga agacgacgaa cggcatcgcg ccaagcgcca tgaagacgat gcctgcccat
    15841 tcgatggacg ggctcttgaa gaagccaaag ctgtcgtcat gcgacgagta gccgccggtc
    15901 gcgagtgtcg gcagcgcatg gcagacggca tcgaagaatg tcatcccgag cgccgcatag
    15961 acgaccgcgc acgcgacgat cagcaccaga tagacacctg ccgtcgccgc gacgagctgg
    16021 aaggcgcgcg gtagcacctt ctccgatttg tccgagcttt cggtctggaa aagctgcatg
    16081 ccgccgaccc gcaggaacgg caacatgatg atcgcgatca cgatcatgcc gaggccgccg
    16141 aacccgcaca ggatcgctcg ccagagcaag atccctcggg gctgcttgtc gaggttcgga
    16201 atgatcgtcg atccggtcgt cgtgagcccg gacatggcct cgaaatagct gtccgtgaac
    16261 gagaacccat ggccgacgaa gggcatcgcc gcaaaggccg tgatgacgac ccaggagagt
    16321 gtcgtcagca ggaagccttc gcggatgccg atccgtgcat gcccgtcgtc gtaggcgacg
    16381 agcgcgagca ggccgccgac gaagaatgtg ccgatcgagg acgcaaagaa gaccttccag
    16441 gtctgctggc cgtcggcgat gtcgacggct gcggacatga gcatgctcgc cgccacgacg
    16501 cagagcatca gcccgagcac aaaaagtatc ggcttgatct gcacgtcggg ccgcggcctc
    16561 gcgaaaacgt ctcttgtctg ctcaggcggg cggcagggcg cacgaggccc cgccgctggc
    16621 atcattcagc cggtcgtgga aatcgatgcg gatcaaagca tcgtgcaaag cgtcgcggcg
    16681 ctcgacttgt cgtggctcgg cggcgcatcc tcgacgttca gcgccttgac gacgccgttc
    16741 tcgacgatca tcgagtagcg cttagagcgt gtgccgagac cgcgcgatcc gaggtcgatc
    16801 tcgaggccga tcttcttcgc gaaatcggcc gagccgtcgg cgagcatcag gaccttgccg
    16861 tcggccccgc tgtccttcga ccactgcgag aggacgaaaa tgtcgtttac cgccgtacag
    16921 gcaatgacat cgacgccctt gcgcttgatc tcgtcgtact ggcggacaaa gcccggcagg
    16981 ggcgacttgt ggcacgtcgg cgtgtaggcg cccggaacgg cgaagagcgc cactttccgg
    17041 ttcgcgaaca catcggccgt ggtcgcatcc ttcgggcctt cggcgcccat gatcttgaac
    17101 gttgtctcgg gcaggcgatc tccgaccttg atggtcatgc tacgctcctt gtggtcttga
    17161 tgctgggcct ggactgacat gaaggggtcg catcgcacga gagctcgtcg atgccctggc
    17221 gcaatcgccg gcacattatc gccgtggcgc gaggcggcca agccgcattt tcgcgagcga
    17281 cctttcgctg gaaccccgcc aactcagcgg gccgcgatct cgaaggcatc tagccgctcg
    17341 accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc cgtggacctt tcccgccgac
    17461 gcggatggta ccgcgaggat cggtaccggc ccacgcggtc ccggcacctc tgcccgcatg
    17521 ttgcgatgat tgtggccgtg gagaacaagc tccgccccat gccggcggag cagagcctcg
    17581 atctccttgt cgtcgtcgag ccctcgcagc gccgtcgtct gccccggtag cgggggatga
    17641 tggatcatca tgacgcgcgt gagcccctcg gcagcgagct gatccaaggt cagcgcgagg
    17701 cgccggcgct ggctctcgcc gacgccgccc attgcggaga tcggacgtgt cggccgcgcc
    17761 gagttgacgc caacgagcgc gaccgttccg agccggcgca catagggaaa gagatgcagc
    17821 gcgcgcgggt ccgacgcgct ttccaggcca tccctcgcct cgaccattcc catgtagtcg
    17881 gaccagacgt cgatgccggc ctcgcccggt cggcggcgcg tatagaggtc gtggttaccc
    17941 ggaatgaccg agacgcggtc tggagggccg aggcgggcga gcaggccgcg gaccgcgacg
    18001 atctcgcgcc caagtccgag attgacgagg tcgcctgtca ccgcgatgtg gtccggacgc
    18061 agcgcggcca cgtccccaac gatccgttcg agggcgtcga gcgagtggat gaagcgacgc
    18121 tggcgccgcc agccgatgcg gccgagcatc cgcttcaggt tcagatcggc cagctcgatc
    18181 tcgggctcga ccggcatatg gatgtcggag aggtgcgcga gcctcagagt tccggtcatg
    18241 acctgggctt cagcttcgcg agcgccgcga aggggttgtc gcgcgaggcc tcggtgcccg
    18301 tactggtctg gtcgagggtg gcgcccggca ggcgcggata ggggtcgagc gccaggatca
    18361 gatgctcgat gatgggcggc ccgatcatca gccggccggc gacgatcggc tcgggatcat
    18421 cgggctcgtc cggatcgaac tccatctcac tcgcctcgat ggcctcgatc tcggacgcgt
    18481 cgcgaaactc gactgtgaaa ggctcctcga tggtctcctc gaccggttcg agcgtgatga
    18541 cgcacgactg tgtgaccgtg gcccggagct tgccgtcggc gaagtatgcg ccccctgcca
    18601 tcggccggat ctggtacttg acggcgagcg agcggcatcc gcggatgccg atctggcgcg
    18661 cgatcgctgc gcattccgcc tcgctcgcgg cccgctcgac ccgcacgccc ttcggaccga
    18721 tgtcgtcgat ggcatggctc caggcgaggg cactcgacgg ctcgtcgatc gaatgcttgt
    18781 catctgcggg catgcggcgc actcccatcc tgaacgaggg ccagggccgc cgccgcggac
    18841 agatcgaggc ggccctcggc gacactttcg agacgatttt cggcgagcgc gcggcgcagc
    18901 gctccgatgt atcgggcgag ggccgttgcc ccagccggtt ccacctgcgc gccgtttcca
    18961 acgatccggc cgaggaatgc tgccagcgcc gcctcattgc ccgggtactc ggcttcgacc
    19021 gcggcaagtt cacgccggcg ctcgaacagg gcggccgccg cctgcttcac ccggcgcggc
    19081 acacccatgt cgccgatgcc gatctcgcgg atgctgtcgt ccatgtcggc gacgtaggct
    19141 tcggcgacat cctgtgcgag cctgtcgccg gcctcgcctt cgagttgcag gcgctcgatc
    19201 agcagcgccg tgtgcaggat gatcatctcg aggcgtccca tgaccgtatc cggcacaccc
    19261 atgccgcgat agaacgcctc gctcctcgcc tgcgccacaa tcgagccgta aatatcttgc
    19321 gcgatccgcc gcttacgagc gcggctcctg atccagtcga gcacaggtcg gtctccgttg
    19381 accggggcgc acggcaggcc agggcctgcg gcgtccggtc ggtgggttca atcggtctcc
    19441 cgattgcagt tggtcatact tgaggctagc gctcgggaga taggcagtcg cagtgattct
    19501 gttaagttgc ggaatgtcgc gggcaatccg gcgtgcggtc gcgcagtcgc gggggtagag
    19561 ttggcaatcg atcgggggaa ggacatgcag cttcgccggc ttacactcgt caggccctcg
    19621 cttgccctgg cgctcgcgct cgtgctgacc gcctgctcgt cgcagattac caagcacggc
    19681 catcagttcc aggaaaccga catcgcccag atccagccgg gcatgagcgc ggaagccgtg
    19741 cgcatggcgc tcggcacacc ggcaacgacg tcctcggtcg gcgcaggcaa cgccttctac
    19801 tacatctcga gtaccacctc gcagacggcc ttcttcaagc cgaccgaggt cgaccggcgc
    19861 gtggtcgccg tctacttcac gcccgttggc agtgtggagc gcgtggccca ctacggcatg
    19921 aaagacggcc gcgtctttga cttcgtgcga cgcgagacac cgagccatgc gcgcgatgaa
    19981 ggcgtgctga agtcactctt ccgcaacctc ggccagaagc agctcttcgg cgattgagcg
    20041 gcacccttcg cgaagctgcg cacgcctgtt ctgtcgtgca accgatcgcg cttacagtcc
    20101 cgcgcgcggg ttcagcggtg tagcccagca gacaatcaat ctgatgcggg cctgcaactc
    20161 gaggaatccg gcatcaaagg tttggctggt aagtatcacg cgtcggggtg acttcgtcgc
    20221 tcgccgtcga gtgtccggac atggccggtg ccatcgttgc tcgcgtgatc tgggccgagc
    20281 ggcaccttgc cggagccgcc cggaacatcc agcacgtacg tcggcagggc gagccccgag
    20341 atccgtccgc gcagcgcttt cacgagggcc tgcccttcag cgatcgtcgt gcggaaatgg
    20401 ctcgttccgg gcgcaaggtc tgcatggtgc aggtagtagg gtttcacgcc gaggccgacg
    20461 agcgtgccga acagctcggc gagcgtcgcg gcgtccgcgt tgacgccctt caggagcacg
    20521 gtctggccga cgagcgccag ccctgcggca gcaagccggc ggatcgcgga acgtgcggcg
    20581 ggcgtcagct cccgggcgtg attggtgtgg atgccgatcc acaccgcccg gtcggcatga
    20641 cgaagcgccg ccacgagctc gtccgtgatg cgctcgggat cgactaccgg cacgcgcgtg
    20701 tgccagcgca ggctcgctac atgcgggatc gcaccgaggc ccatggtaac ctccgcgatg
    20761 cgccgcggcg cgagcatgaa tggatcgccg cccgtcagta tcacctcacg gatcgccggc
    20821 cgcgcccgga tgtagtcgag cgcagggacc agccggtcgc cgaccatcgg cggcgggccg
    20881 cccgggccga ccatctcgcg gcggaagcag aagcggcagt agaccgggca ggcgagcgtc
    20941 agcttgagga gcacgcggtc gggatagcgg tgaatgaggc ccgagagcgg ctcgtnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn ncacggacgc gagatcgagc tcgcgcgggt cggggatgaa
    21061 ctggcgcgcg atcggatcag cgggattggt cgcctcgatc agcgcggcca tggccggcgt
    21121 cacggcgacg gcgtagcgct tcgccacccg gtcgaggcgc tgagcagcgc tcgccggaac
    21181 gagcccttcc cgttcaagat cagtaatcgt tcgcagggtc gagcgagagt tcgtgatcat
    21241 ggcgcgcacc gtctagcacg gtttccgccg gacgtggtca gcctctcgtg ccgggcgcga
    21301 cagccttggc gaagcgcaga tggccttcct tgacccagaa ccggcaaccc tctgagccgg
    21361 cgagagcctc gagccgcatg ccgatcggca gccgcagcca atcgcgggcg ccaagcgcgt
    21421 cctcgcacac tgccatggcg ccttcgagca ccagaagctc gagcccgcca acgggatcat
    21481 aggcgatctc ggcgccgggt gcccaagtct cgatgcgtac gtcctcgcgg gcatcattga
    21541 agagcggctg cacggagacc tttggacggc ccggcagctc tcttgaggcg gcgcgggccg
    21601 tgtcgatgcg gacgaccgcg cgatctgccg gatcgaattg ccagagcttg acgaatatcg
    21661 tgcagcccag gtcggagcgc ggcgtatgcc gagatcctgg cgggttgcgg acgtagtttc
    21721 cggccgggta atccccgtcc tcgtcctgga agacgccgtc gagcaccagg aattcctcgc
    21781 cgccaccgtg aacgtgtggt gagaagcggc ttcccgctgc gtagcgcacg atcgtcgtcg
    21841 cgcgcgcgac ttctccgccg atacggtcga gcatgatgcg atcgacaccc gccatcggcg
    21901 acggctgcca ggggcgccag gccgaatgct cgacagcacg cttcgcgaag tcggcattga
    21961 gctccatgtc ggtcctcctg ccaggcgggc cgccgcagcg tcagctgcgc tcgggatcga
    22021 gtgctgttgt cccgctcggc cgcccctcgg cgtcgaaggt gagcttctcg accttggctt
    22081 cggccgcctt caagagcttg tcgcaatgct cctcgagcgc cttgccgcgc tcgtggatgg
    22141 caatgctctc ctcgagctcc acatcaccgc gctcgagccg cccgacgatc gcctcgagtt
    22201 ccttcagcgc gcgctcgaag gtcatgtcct tgatgtcggc gaagcggccg cggcggccag
    22261 cctcggatgc cggagcgggt gacgacatgg ggcgctctcc ttcggaaatc gtttcaggcg
    22321 ccgtttcagc ggccggcgtc catcagcccc gcgacgtgca gcgacaccga gttcgcgagg
    22381 gcggcgagat catacccacc ttcgagcatc gagacgatgc gcccgccgga atgcttgtgg
    22441 gcaatgtcgg cgagcttgcc cgtcgcccag gcgaaatcgt cctcgagcag tcgcaggttc
    22501 gcgagcgggt ccgcctcgtg ggcgtcgaat cctgccgaga tcagcacgag atctggcgcg
    22561 aaattgcgca gcgacggcag gatgcgggct tcgaacgcct ccttgaagct gctgccgcca
    22621 tcgcccggcc ggagcggcgc gttccagatg ttgccgacgc ccgtctcgga gagcgcgccg
    22681 gtgccgggat agagcggcat ctggtgcgtc gaggcgaagt agatgctctt gtcagcgtag
    22741 aagatatcct gcgtgccgtt gccgtggtgc acgtcgaaat cgacgatggc gatgcgctcg
    22801 gcgccgtgtt tcttctgggc atagcgcgcc gcaacggcga tgttgttgaa gatgcagaag
    22861 cccatcgccc gggccgtttc cgcgtgatgc ccgcatgggc gcacctggca gaaggcgttc
    22921 tggacccggc cggcgatcac ctcgtcgacg gccagcagac cggcgccgac tgcccgcagt
    22981 gccggctccc aggacgtcgg cgagagcacg gtgtcgggat cgaggcgcac cgcctcgtcg
    23041 tccggaccgg ggcgcgccgc cttcacggcc tcgacatact gatagggatg aacgcgggcg
    23101 atccactcct cgacgtctgc aatgagcggc gcctcgcgcc ggacaagcgg tttgaactgc
    23161 tcccgcggca gcgcctgcca gtggcgcagc accggattgt cgcgcgcgag cttctggcgg
    23221 aacggcggct cctcggcccg ctcgggcgcg agcgccggat cgagcacacg catgcgatcg
    23281 ggccgctcgg gatggccggg gccggtgtcg tgctcgacga agcagggatg ggtgacgagg
    23341 agcgttgcca tcagtgtctc ggttggtaga cgcgatcggg ttctcttgca acattacacc
    23401 ggctctcccg gattcacacc cgccccttcc acggtaccag cagccgctcg gcatagcgca
    23461 tggcgagctc gaaggcgtag gcgacgaagc cgatggccag gatgccgagc acgacgacgt
    23521 cggtgcggag gaagttcgac gcgttgtgca ccatcttgcc gaggccggca tcggccgcga
    23581 ccatctcggc ggcaacgagc gtcgtccagc cgacgcccat gccgatccgg aggccgacaa
    23641 ggatctcggg aagcgcaccg gggacgatca cgtggcgaac gacctgcatc cggctcgcgc
    23701 ccatggagag cgccgcctgg atctgctcct gcgagactga gcgcacgcct gcccgcgcgg
    23761 cgagggcgac cggcgcgaag atcgcgagga atagcaggat gatattgttc ttctcgccga
    23821 tgccgaacca gatgatcatc agcggcaggt aggcgagcgg cggcagcggc cggtagaact
    23881 cgagcggtgg atcgaagacg ccgcgcgcga tccgcgatac gcccatggcg atgccgacgg
    23941 ggatgccgag cgccaccgcg agcagaaatg cgctgccgac acggccgagg ctcgcgagga
    24001 aatggccgac gagcgtgctg ttgtcgagct caccctccat ggcctggcgg aaggcgagcc
    24061 agatgctctc gggcttcggc aggaagagcg gcttcagaag cggcgtctcg ccccagtgaa
    24121 agccgttctc gaaggagaag gccgggtact tgaggccctg acgggttgcg agccaccaga
    24181 ggatgaagca caaggcgatc gtcacgacgc tgatggtgac gctcgagcct tcgccgagca
    24241 cgcggtagcg cgatgtcctg gggcgagcca caggcgtgga ctcggtgtcg cccgtcgagc
    24301 ggatcgattg gtcagccatc ggagactcca gacgtgatgc gcgttctttc ggctcgccgc
    24361 aaccactggg ttggtgcagc ccacggctgc aactgtgttt tgcctaggcg gggtcgaatc
    24421 aggtaaacgc gtgacgaatc aaccgcttcg ggaggagctt catggccgac aagacaacct
    24481 tggtcctgcc ggggcggaaa ttcacagttt cgaaggtcct gaagcgcaca ctgcaaatcg
    24541 ccgtcgccgt cagccttacg tcgctgctcg cgctcgtcgt cgtcattgcc aacggcatgc
    24601 tgatcggacg tggcgccaca ctccaggcgg gcgttgccac ctggctcagc ttcatcaagc
    24661 gacccgacat ccagaccacg atgctgctca cggccgtggt gagcgtactg ctcgtctact
    24721 ggcagaggga tcacgagaag cgctgacatc agacccgccg ttggatatcg gccagcacct
    24781 gctctcgcac cctgatgaac tcggggtgcg atttcacggc gcgggcgtcg cggctctcga
    24841 tgaagcggcg gccgaagtcg agcgtgtacg acttctcgat cttgccgggc cgcggtgtca
    24901 tgacgatgag gcgcgtcgcg agaaagatcg cctcttccac cgagtgggtg atgaagaaga
    24961 tcatcttgcc cgtgcggtgc cagacgtcga ggatgagctc ctggacctgc tcgcgcgtca
    25021 aggcatcgag cgcgcccaac ggctcgtcca tgagcagcat ttccgggtcc gaggcgagcg
    25081 cgcgcgcaat gccgacgcgc tgctgcatgc cgcccgagag ctcgtagatc atcttgttcg
    25141 cgaactcgtc taggccgacg agggcgagtt tctcgcgtgc gatcgcgtgg cgctcctcgc
    25201 ggggaatgcc acgcatcttg aggccgaagg cgacgttgtc gatgacattg agccacggca
    25261 tcagggcatg gcgctggaac acgactccgc gatcggcgcc cggccctgcg acgggatggc
    25321 gatcgagcag cacctcgcca tccgagggcg cgagaaagcc tgcgatcacc gagagtagcg
    25381 tcgtcttccc gcagcctgag gcgccgatcg ccacgaggaa atcgccggcg gtcatgtcga
    25441 gattgacgcg cgatagtgct tccgtgacgc cggtgtcggt cgcatagcga acggcgaggt
    25501 ccttgatttc gagcttggcc acaccgtcgc cttctgagga acgcctgccc aactttgaat
    25561 gatgggtcca gggacgagtc cccggtcggg agttcgaggg gcgagcccct cgaacgcttg
    25621 atctcaaagg ttgcgggctc ttacttcgcg gcggctgcga actccggcgt cacgaacttc
    25681 gcatagtctg ccgcgacgtc cgtgatccgt ccctgctcct tcaggaaccg cgccgtatcg
    25741 agcatggccc gggcgacacc accctcggcg ccaccaccga gccaggcctt cgaggcctgc
    25801 acctcggccg tcgggaagat gtagtcgttc agcactgcgg gcacattctc gggcttggcg
    25861 ccgacgatct tggcgattgc cgcgacctcg gccgaacctg ccacccactt cgccttgttc
    25921 gcccggaatt cggcgtcctt ggccgcgatc atcttgacga gtgtcgtcac gaattccttg
    25981 tgctgcgcgg cccaggcgcg attgacgatc agggcatcga aggtcggggc gcccttcttg
    26041 gcgatctcgc cagcggaaat caggacggtg ccgcccttct tgatgtttcc gagaacgggg
    26101 tcccagatga aggtcgcgtc gatgtcgccg cgctcccagg cggcggcgat ctccggcggc
    26161 cgcatgttga ggatgttgac ctccttggcg ttcacgccgg catccttcag cgcgaacagg
    26221 agctggtagt gcgccgtcga gacgaagggt gtcgcgactt tcttgccctt gagtccggcg
    26281 agtgtcgtca cgcccgactt cttcgaggcg acaagctgct cggcgttgcc gatatcgtcg
    26341 aggatccaga tcacctggat gtcgatgcct tgcgaagccg cagctgtcgc cggactggag
    26401 cccacctcgc cgatcggcac ctcgccggaa gccatcgcct tgatgacatc gccgccgccg
    26461 ccgaacatct tccagctgat cttgtagccc gtcttcttct cgatctcgcc cgcggccatc
    26521 agcacccgat agggtatgat catgtcctgg tgggcgaagg tcacctcttt cttgggctgg
    26581 gccattgcgg ccgtggcgcc gagtgccagg ccgatcgccg ctgccatgcc ggcactcacc
    26641 attcgcagag gtctcatgca acatcctcct cgtgctcgag acgacgtcgg tggcgatttc
    26701 gcgtcgatcg gccgaggcag gcaaggccat cgtgtggaga cgccaacgac cgatcgaacc
    26761 tagcacggac cgacgctccg gcaatcgcca agaggtacgc gtggctcgat tcgatgggct
    26821 cgaccattgc tgaggcaaca gcgcggtcac tttatcttgg gcgcggcggc agcgatcgcc
    26881 gtctcaaggt cctcgcgctc ctcgcagccc gtcgggcaga gcctgacgag aatggcgagg
    26941 tggccgcgcg ccttctcgag atcgccggtc tcgacataga gttcgcccag atactcgtgt
    27001 gcgctcttgt gctcggggtc gaagtcgagc gccttcttgt agaacgtcag cgcggtcgga
    27061 tagtcgccga gcttgcggtt ggtgaagccg agcaggttgt agacgtcggc gtgctgcgtg
    27121 ccgtcggcga tgcggttgag ctcgtcacgc gccttggcaa attccttcgc tttgatcagc
    27181 gcgcgcaccg ccgtgaggtc gggcgccgtc gtgcttgtca cgttgtcggc ggcgccggcg
    27241 ggcccgatca acgagatggc tgcggcaacc aggaccgcga tgacgcgtgt cgtcattccg
    27301 ctcaaggtgt tctcccgtcg ctcgacgtgc tcgatgctat catggcgggg ctctgtgatc
    27361 agcccgtgaa atcactccgc tcgaggagcc acgaggtgac gaggccgagg agcagccccc
    27421 agaacgcggc gccgatgttg aggaccggga tgccggcgac cgtgacgagg aacgtgacga
    27481 gcccgccaag ctggaagcgg cccgaaaacg ccgcgacgaa cgccgtctgc agcacgcgca
    27541 gcatggcgag cccggcgagc gtcgccagga atccaggcgg tgcgccgatc agcacgcgcg
    27601 tgacgatcgg cgagaaaatg ccgacgccga gcgcgattgc gccgaccatc acggcggcgg
    27661 cgaaatggct ttcccgggcc ccgctcgagg tgatgatcgc attggtcggc ccggtcaggc
    27721 aggtcgagac cgtgccaacg agcccggcgg cgagcgagaa gacgccgcag gccgcggagc
    27781 acgcgttgat cggcgtgtcg tgccccgcga ccttgagcac ggcggcgccc tggccgttct
    27841 gcacgacgag caccatgatc gcgagcggga tcacgagctc gatcatggcg ccgatcgaaa
    27901 aggcgggtcg gaagagcacc ggctcggcca taacggcggt gccgctgatg gccggcaggg
    27961 tgccggtcgc cg
//
LOCUS       DAORNH010000020        26960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1367_length_26960_cov_2.680691, whole genome shotgun sequence.
ACCESSION   DAORNH010000020 DAORNH010000000
VERSION     DAORNH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26960
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_26960_cov_2.680691"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PK264_03770"
     CDS             <1..898
                     /locus_tag="PK264_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD75046.1"
                     /translation="RRAAREVESADQTLRPFWRLGRARPLIEGALWLLIGIAIVVPLM
                     SLIATSLVPALGVPLRAPTATLESFREVLFRQDVTRRAALNSLVLSATAAVLSAGLAI
                     VVAYGIERVARRWRSALLVLIELPYAIPGIVLAIACILLFLRPLPLIGISLYGTPAII
                     LVAYLARFQALALKPTLAAMALVSREPEEAAALEGATMCQRLRHVVIPSLAPAAVAGG
                     LLVFLLALNELTVSALLWTAGTETLGVALMSLEDAGLASQAAALGVVATVLVALIMLA
                     IDRIARGTSADAVPWASISGSR"
     assembly_gap    1065..1074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1075..>1651
                     /locus_tag="PK264_03775"
     CDS             <1075..>1651
                     /locus_tag="PK264_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD75047.1"
                     /translation="ADVRATLEAEIVRGAFKPTVGAMTAADPAFSELCGAKGWLGMTW
                     PKAYGGHERTALERYVVVEELLAAGAPVMSHWIADRQSGPQILKHGSERAKRLILPEI
                     AAGRCYFAIGMSEPDTGSDLASVKTKAVRTDEGYRVTGRKVWTSFAHSAHYIIALVRT
                     GGSPEQRQVGLTQLIIDTKSPGVGIRPIINIT"
     assembly_gap    1652..1661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1662..2184
                     /locus_tag="PK264_03780"
     CDS             <1662..2184
                     /locus_tag="PK264_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD75048.1"
                     /translation="GAHEFNEVTFDDVLVPLDMTVGQEGQGWAMVTSELAFERSGPDR
                     FMSTFPLLVETFGAMGPEPAPHSAADLGRLVAHVATLRRMSLSIAGMLEAGQSPVTEA
                     ALVKDLGTTLEQSIPEIARRVLPIEPRREVGNDDRYIELLELSQLQAPSYSLRGGTRE
                     ILRGMIARGLGLR"
     gene            2192..>3104
                     /locus_tag="PK264_03785"
     CDS             2192..>3104
                     /locus_tag="PK264_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD75049.1"
                     /translation="MSETRDMMLASFDRVVADHLDRGAREAADLQTAKEAGAEPWPSE
                     VWAALEQQGMTALGEIAEGDLGFVDSMALVRRSAYHAVPLPLAETFVARRLLARAGLE
                     LPDGVLTISAPASMHDVVSVREGQITGRIAHVPFARGVRHLVIPVGSGRGAALALVNI
                     ERAIAHASISKAGEPRDTVDLGRAMPVAVGTMHDAPLIVEAEGALMRAVQMSGALSRV
                     LAEALTWVSDRVQFGKPIARFQAVQHLVAELASECAAASAASDMAVEASVSAPDRFSI
                     AVAKARVGEAAGRASAIAHQLFGAMGFT"
     assembly_gap    3105..3263
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3293..>3567)
                     /locus_tag="PK264_03790"
     CDS             complement(3293..>3567)
                     /locus_tag="PK264_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75050.1"
                     /translation="TGSTSQPFATNLHAAQLTSPLLAVLLEDLEAHGRRHPVPSLAPK
                     APPPPAVAAAPATAAPASAPPKADPRAAKPRLQTDEARRRQIFMGN"
     assembly_gap    3568..3708
                     /estimated_length=141
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3709..5855)
                     /locus_tag="PK264_03795"
     CDS             complement(<3709..5855)
                     /locus_tag="PK264_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRD75051.1"
                     /translation="MLAVLRLISRAIELALAVPLRLLPLVGRLLALPFHAVRHLLVAV
                     SFNRKLGPLRHVATAAIAYLAFALVLVYVVAPIRGYVGSYYLADKLRYDAERWLATAI
                     YDNRGNFIGSFDPRLDSRRDVNYTDAAIELGDYTALPDHKSVPVNEVPEHYWRCLAFH
                     EDRNIGTWINPFGIDLYGVLKIPYTSIRRSIALRRPSLGVGGSTLPMQLVRVIDKTPP
                     DASHGVFAKLSRKFREWWLAPVIYRELTRDGDDTPLKRWAANHLWLAQRTGGSSLHGV
                     EITSQIVFGKDAKDLTVAEQFVLASAVNKPIILLSGDEKLNEVRIDRWRYITEVRART
                     CAEKLVSDPELQKATIFELVQLAGGPPDPKAKPLLRATLVAHTPALAARAVANPFIRA
                     NALLPAARFGIREEMKQSWGFGWRDYVRGVTVTLDVAENLAFAEKIRAELKRLHAQHG
                     AKFAPGYTIDPAMLVAGATLKIPNVIVVAANARGEIVRYFELGETAAYFGSPFARDAA
                     TGFYAPAREQRRVASTGKILIAIALANEARDVISSLYPDALAPDRGGDTCERGSGATP
                     GHRRAIVAFACSLNRPIEWRAAQLGQERLRRLIDALGFAMPPAPSASEVTPPSTAAVR
                     GLISGSPRRVHHMLIGEGAKPVRLPTLVKSYDYTSRESALAAMHSAESAIRPNTLIRP
                     HAHQLIKAFLQAPLCHWAGGIAHGTLKSLSTWCA"
     gene            complement(5998..6645)
                     /locus_tag="PK264_03800"
     CDS             complement(5998..6645)
                     /locus_tag="PK264_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75052.1"
                     /translation="MKAGVFALTGATLVATGLLLVSATPVVRSQSAAPKIELTPEELK
                     EREDRKGCKIQICSAFALKKPGPDIGCNVYKTWRKEQIVKMVSKGGLQWLWGKARCAA
                     DIKLPRAGLLKAVEGGEHEMVLEKHVATCDIERPGETPFTMKVTFQPKVTFKGGKAVK
                     ASLNWGDVDAPIVAKTAIWTLTAADNKLGVLQSSMVEDINDFMGPKCDEVKGELK"
     gene            complement(6730..8040)
                     /locus_tag="PK264_03805"
     CDS             complement(6730..8040)
                     /locus_tag="PK264_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRD75053.1"
                     /translation="MPLAEALAERAAAGTPVRVGLVGTGQMGTDLMVAIAAMPGIEIV
                     VAADMTPERIATAAGIAGAACRTPEIVTSLADVSRTIGRGRLASVQKPDLVFAAPEVD
                     VVIDATGNPSAGAAVALGAIAARKHIVMMNVEADVTAGAYLASEAEKAGVIYSLGAGD
                     EPTAALELINFVRALGYPVVAAGKGKNNPFRIDATPDAYREEARRRNMNPRMLVEFVD
                     GSKTMVEMAAIANAAGLVPDIPGMHGPAAPREALQTTFCPKEDGGLLSRRGCVDFTVA
                     KGVAPGVFAVAEMRHPRVRERMADLHLGPGPYYAFFRPYHLTSLEVPLTAAAVVIHGE
                     CHMRPRPIPSAEVGCVAKRDLTPGETLDKIGEYSYRAFTLTREDAESRAALPVGLAEG
                     AKVTRRIAKGELLTYAAVAPDETRPIAIARRRQDAMVAGLLATR"
     gene            complement(8154..>10187)
                     /locus_tag="PK264_03810"
     CDS             complement(8154..>10187)
                     /locus_tag="PK264_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRD75054.1"
                     /translation="AYGVAAAGLTYFNKELHELEIEEVAYLAALPKAPSNYHPFNKTQ
                     AATIRRNWIIDEMAKNGFIKDEEAKTAKAKPLTVNIRPFGTRVYAADYFAEEVRRQLV
                     GFYGDDGLYGRMERISSGDKRVNGGLSVRTTLDPKMQAMARKALVDGLVAFDRVHVGW
                     RGALKNIDVSGDWGPVLGAIDVPSDITPWRLSVVLELQPKTAIVGLRPPKLQDGTFAP
                     AIERHSVPLEEMKWAKVGAKKAEPKAPSEVLQVGDVIYVSPKDPANPAGVWSLMQIPE
                     VGGGIVVMDPHTGRVLASVGGFSFAASQFDRAVQAKRQPGSSFKPFVYAAAIDNGYKP
                     TSIVLDAPITIETGRGDEIWTPQNYGGKYYGPSTLRLGIEKSRNQMTVRLAQDMGMPL
                     ITEYARRFGIYDDLMPVLSMSLGAGETTLLKMTAAYAMLANGGRKVKPTFIDRIQDRW
                     GRSIWRHDERECLGCKAERWTGQAEPDVPDDRPQIIDPHTAYQITAMMEGVIQRGTAT
                     ILKSFISDKMGLAGKTGTTNDSRDVWFVGYSPNLVIGVFVGYDTPRPMGQIATGGAVA
                     APIFGNMMKYALADKPAVPFRRPPGLKMVWVNLKTGLPTAPRSPESVEEAFKPMEDPD
                     EPHQVIGFTADAAAFGGEGATPPPVPPPTAGAPPAPGSPFTTTPRRPAGSPFW"
     assembly_gap    10188..10279
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10280..10790)
                     /locus_tag="PK264_03815"
     CDS             complement(<10280..10790)
                     /locus_tag="PK264_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRD75055.1"
                     /translation="MRAPTYPPPPAQPTRKKKRRRSWLLSFLGFAFASGAVLFLAGSA
                     IAGYLIWKASQDLPSYESLANYEPPVMTRIHAHDGSLIAEYSRERRIFVPINSIPKRV
                     INAYLSAEDKRFYEHGGIDFQGVIRAAVKFVEAKIKGRGGLQGASTITQQVAKNFLLT
                     SDRTFDRKIR"
     gene            complement(10935..12221)
                     /gene="serS"
                     /locus_tag="PK264_03820"
     CDS             complement(10935..12221)
                     /gene="serS"
                     /locus_tag="PK264_03820"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRD75056.1"
                     /translation="MHDIKWIRDNAAAFDKGLKNRGLAALSSKLIAMDEERRRHVTSL
                     QDVQARRNAASKEIGKAKSARDEAKAQALMAEVATLKDQLSAGEAREKELTAALEREL
                     SIIPNLPLADVPVGSDEKGNVEKRKVGTPKSFNFTNKPKQHFEIGEALGLMDFETAGM
                     VSGARFVFLKGQLARLERALANFMLDIHTGEFGYTEVVPPMLVREPSVHGVTSLEKFR
                     EDLFGTTNGFWLIPTAEMPLTNLVREKILDEAELPIRVTAWTPCFRSEAGAAGRDTRG
                     MIRQHQFSKVELVSITTPEESLAEHERMTSCAETILSRLGLPFRTVTLCTGDMGFSAQ
                     KTYDIEVWLPGQDAYREISSCSVCGDFQARRMLARYRPKGAKDVRFVHTLNGSGLAVG
                     RTLIAVLENYQEGDGSVAIPEALRPYMGGLSHIGRG"
     gene            12409..12756
                     /locus_tag="PK264_03825"
     CDS             12409..12756
                     /locus_tag="PK264_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD75057.1"
                     /translation="MTIKRHEPSQILSKVVEANGFVFTAGITADDASQNPQGQTKQIL
                     TTIDKLLKMCGTDKSKVVSATIWVSDIRFRDQMNIEWNAWTGGKDLPGRACIEAKLAD
                     PRLTVEISVIAAK"
     gene            12762..13112
                     /locus_tag="PK264_03830"
     CDS             12762..13112
                     /locus_tag="PK264_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD75058.1"
                     /translation="MRIDRHEPEAGVLSNVVGAGGFVYTAGSVADNLDLDVEGQTREA
                     LAYIDQMLARCGTDKSKVVFATIWLADIRLRDAMNKAWLAWVDKGNLPARACIEAKMA
                     NPAYLVEIAVVAAR"
     assembly_gap    13165..13174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13240..13680
                     /locus_tag="PK264_03835"
     CDS             13240..13680
                     /locus_tag="PK264_03835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD75059.1"
                     /translation="MSERMPPGRRADYKHFLPIPTRWMDNDVYGHVNNVTYLSYADTV
                     VNRYLIDVGRLDFNADPVIGIMVETMCRFHKSIAYPDLIDCGLRIGKLGSSSVRYEVG
                     MFVAPDDVARAEAHCVHVFVERATNKPVPIPEKIRSALARIMRG"
     gene            13883..14347
                     /locus_tag="PK264_03840"
     CDS             13883..14347
                     /locus_tag="PK264_03840"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRD75060.1"
                     /translation="MAFAGSMDLFTMTIPNRISLALIAAFVVAAPYCGLTVTQIAWHL
                     GCGFLMLVIGILMFSRGWLGGGDAKLLAAASLWLGFEHLLGYLVLVAVAGGMLASTIM
                     LYRSMTIPLCLIGQGWASRLHEKTSGIPYGIALAAAALWIYPSTTWFKVLVA"
     gene            14518..15327
                     /gene="cpaB"
                     /locus_tag="PK264_03845"
     CDS             14518..15327
                     /gene="cpaB"
                     /locus_tag="PK264_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HRD75061.1"
                     /translation="MKKAQLIGLAIAGACGVGAFLMMKNVVNKPQVIQKEVSTNLVEV
                     LVARNDIGLGQVTSESHFRWQPWPQDNIQPGFVTRRAGQSTSGMKDYTGRIARAPIMG
                     GEPITAIKLIKAGEGGVLASILPEGYRAISAKITDDTAVGKMILPNDHVDVILIRRLR
                     GRGGAEEHVSDTLFRNIRVLAIGQVIQVGDPSKKGADGTTATLQLTSRQAELLALAKS
                     MGEISLVLRSVADFKKDADGNAADAENLKSQRGNAVRVLRYGVRKRDYGVN"
     gene            15462..>15725
                     /locus_tag="PK264_03850"
     CDS             15462..>15725
                     /locus_tag="PK264_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75062.1"
                     /translation="MRKTLNSAWIPGLVGLAGIAALAFAPGLGSSALANGPKRGAPMP
                     GTVASFAPEHQSTLRIRESDRLPLHKGVRLGRNKSMLVELPREL"
     assembly_gap    15726..15751
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15752..17044
                     /locus_tag="PK264_03855"
     CDS             <15752..17044
                     /locus_tag="PK264_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HRD75063.1"
                     /translation="AVVQSSNRVYLIGKKTGQANVFFFDANGEQIVTLEVSIEHDTAV
                     MEAMFTRLIPGSRIKAEMLNETVILTGHVRNPADSARASDIASRFVVTPSPEANGKHK
                     DKVINLLAVEGEEQVMLKVVVAEVQRSILKQFGINIGAELNVGNMALGLLTANGLPLT
                     SAAGLGRLPVAGSDLASPMGQGCGSVFDLAKTGVIGGSGPYGGWGSGNHCVSHVMRAL
                     ERDGLVRTLAEPNLTAISGEPAKFLAGGEYPVPVVDSLGKLSVTFKEFGIGVTFTPVV
                     LSEGRINLKIEAEVSELTDAGSVTLSSISIPALKKRQAKSVVELPSGGTLALAGLISD
                     DTRQNIDGLPGLKDVAILGTLFRSRDFIKRETELVIMVTPYMVKPGRRQDFARPDDNL
                     APASDIKANLLGHLNRVYGRSSAMPAGDLKGDYGFIVD"
     gene            17066..17821
                     /locus_tag="PK264_03860"
     CDS             17066..17821
                     /locus_tag="PK264_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HRD75064.1"
                     /translation="MTDILHMPPSTTASKRPLVKVGASMIAAMALTVTVAGCKHGEEP
                     SGHVAGWTLVDPSHRHPIVVHQKPTTIAVRVARGADGLNPHQRSQVVSFLERYRGVGA
                     GNSRLVISVPSGAPNEVSAMSAVTDIRHLARDLGFDESVIGVEAYHMEGDNAPPVKLS
                     YTHFVAEAPHCGQWPTNLAEDPRNVPYPNFGCATQRNFAVMIANPADLVGPRGMTPRS
                     AERGDQVWERFVKGESTITKKDNDEKNVVKGAN"
     gene            17833..19146
                     /locus_tag="PK264_03865"
     CDS             17833..19146
                     /locus_tag="PK264_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtpF protein"
                     /protein_id="HRD75065.1"
                     /translation="MSRQAKIQTAPADEPGGRELAVMEEPGFPVAAGSEKARPIPRIS
                     IQAFCESSRTAEALQVAAEDRRLAKAHVSVHMGGVQAAVAHYQESPTPNLIVLDSALP
                     RAELLGEIDRLAESCDSGTKVVVVGHTNDVVLYRELLKRGVSEYLIAPISPIQFMESV
                     SNLYNDPDADPVGHVFAFIGAKGGVGSSTICHNAGWAISEILQTELVIADMDLPFGTA
                     GLDFDKDPVQGIAEAISSPERLDEVLLDRLLTKCSEKLSLFSAPGTVDRDWDLAPDAC
                     EMVLDVVRQHIPHVVLDLPHAWNGWVRRMLLAADEIVITAVPDLANLRNAKNIVDHLK
                     GARNNDGPPHLVINMHNTPKRPEIPIKEFAQALDLNPIAVIEFDAETFGEAANHGRMI
                     EELNAKAKAVQPLRDIALTLTKRPLPKVEKKSPLAPLLEKLKLKR"
     assembly_gap    19150..19236
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19237..20665
                     /locus_tag="PK264_03870"
     CDS             <19237..20665
                     /locus_tag="PK264_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRD75066.1"
                     /translation="APAASPAAPKPQEAKAAAPTEARAPKPPAPEPEPQRKHSEEYYD
                     VKTTVFNALIDTIDLTQLAKLDAAAAREEIRDIVSEIIQLKNVVMSIAEQEELLEDIC
                     NDVLGYGPLEPLLARDDIADIMVNGAGTTFIEVGGKVQKTGVRFADNAQLMNICQRIV
                     SQVGRRVDESSPICDARLPDGSRVNVIAPPLAIDGAALTIRKFKKDRLRLDNLVKFGS
                     ITPSVKAILEVIGRVRCNVLISGGTGSGKTTLLNCLTGCIDHDERIITCEDSAELQLQ
                     QPHVVRLETRPPNLEGEGEITMRDLVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGH
                     DGSMGTLHSNSPREAISRLESMIMMGGFALPVKTIREMIVGSIDIIIQASRLRDGSRK
                     VTHVTEVVGMEGEVVTLQNLIVYEITGEDAHGKLIGRHRSTGIARPRFWDRAEYYKDT
                     DKLAIALSDSEVRDSNGNPMGESNA"
     gene            20658..21659
                     /locus_tag="PK264_03875"
     CDS             20658..21659
                     /locus_tag="PK264_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD75067.1"
                     /translation="MLDGQNLQLILGVVIALSVGGIIYVLAYPYLSGEVQTDKRIQGV
                     TEAKSRRSQRKGSQADIASTKRKQVADTLKELEERQKAKDKISMKLRLQRAGLDATPS
                     MFWIASAICGAVCGAGAFFAFPSMPPYVAGAAAFVGALGLPRWILMQMTKWRQAKFLN
                     EFANAIDIIVRGVKSGLPLNECLGIISRESPSPIKEEFQEIVEQQRVGVPLVDCFDRM
                     MQRIPLAEVKFFAIVLGIQAQAGGNLSEALGNLSGVLRDRKKMQGKVKALSAEAKASA
                     MVLGALPFIVMTMVYVTTPDYIALLFTKKMGQFLLVAAAVWMSAGIFIMRKMINFKF"
     gene            21715..22683
                     /locus_tag="PK264_03880"
     CDS             21715..22683
                     /locus_tag="PK264_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRD75068.1"
                     /translation="MVDLIEKLLQPQLVVMVLAAIATFATVLTIVLPALSGDRMTQRM
                     KVMAVERDKMRAARLSDLNKEGQGKLRQAPKGFMQQIVNQFNLRQTFETDEIKNTLKM
                     AGLRGQAPLVAYMFFRLVAPIIVTIGALAYLFFIGNIQYPPIAKFGMAISAGYLGFYL
                     PNMFLSNLVQRRQQSINSAFPDSLDMLLICVQSGMSVEAAFGRVSKEVASQSLELAEE
                     FSLTTAELSYLQDRRVAFENLGKRTIPGIKSVATALIQAERYGTPVSQALRVMAKENR
                     DMRMLEAEKKAAALPPKLTVPMIVFFLPVLFVVILGPAVIQVMKWT"
     gene            complement(22760..23227)
                     /locus_tag="PK264_03885"
     CDS             complement(22760..23227)
                     /locus_tag="PK264_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75069.1"
                     /translation="MMMPEIATRTASQAMAAVEAEIEALASGGDAYDHGAAVATVEKL
                     LALGERVLEIWIETRGQTPTEERREGFRLLALHRQGAKGDPSFNACRETARELAYHYN
                     LIIAEPDHPEIQQRLALAAMLARHLCLFVGGKIEVAGLGEFCCSSRPLRAEGG"
     gene            complement(23214..23633)
                     /gene="rbfA"
                     /locus_tag="PK264_03890"
     CDS             complement(23214..23633)
                     /gene="rbfA"
                     /locus_tag="PK264_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRD75070.1"
                     /translation="MSRQKQTRDTRQGGGSQRQLRVGELMRHKLAEMLARGEIHDPVI
                     TGAVITIPEVRMSPDLRTATIYVMPLGGKDLKPIIAALERNKRFIRGVVAQAVNLKFA
                     PDIRFRADDTFDEVLRIEKLLASPKVRQDVDRKDDDA"
     gene            24007..25383
                     /locus_tag="PK264_03895"
     CDS             24007..25383
                     /locus_tag="PK264_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRD75071.1"
                     /translation="MLFEPLTINGITFKNRIVRSSLGGRSSYYDGTPSPAWVGFERRF
                     AKTGVASIISATIGINARRMSPHEYPSIAHDRFIPAFRTGVRAVQAEGCRYIMQIGDP
                     GGHTQVGLFSEEADSKSSSAGFDFVYGYRNRLTPMTISEIEASVREFASAARRVREIG
                     CDGLEITASKGYLIHQFLNPGINRRKDEYGGSDENRFRFLREIVVAVRREIGRDFLFG
                     VRLAAEDLNSSPVNLRLPIAWPLRHHFRGNGLAETTRHARSLEALGVDYLHIDAGYGF
                     PHPHVNLGKWPLEGFRLYANSVRHLSLKSRARAVALNLLPGPLARLAFGAGWNKKPGA
                     NARYAEVFKKTVKLPVIANGGFQSRRLIEETLTSGKADLVAVGRPLLANPGLIDAFRD
                     GRDEPDRPCTFCGECCAKTATLPLGCYDRSRFASDRDMEAEIMRWSASPPADDEGHLA
                     VELPSAAK"
     gene            complement(25404..>26960)
                     /locus_tag="PK264_03900"
     CDS             complement(25404..>26960)
                     /locus_tag="PK264_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRD75072.1"
                     /translation="RSVGPCAPAGLWQRGTEFRDDCHGSMAIIAAIVMTVLVVLAGGA
                     IDYGRWHAAYNRIQGSIDSAVLASGRAVQLGLSNSEVIAVAERFYSRNRPVGLTRDGA
                     TFEIRNSSQIVGIVDAAVGTTFLNLIGIGELPINIAARADLASGGSSGTNVELALMLD
                     VTGSMCDDGVGPCSSGTKLNALRAAATDLVNIVVWSDQSRYTSRVALVPFSTRVRMAP
                     NGDGDTLMRNLTNMEATWSGWYTFCTEGSGSGGSETNGTWACTRTETNNYSGWRIMPC
                     VTDRTGPEEFTDAAPGPFTWLNAHDGSRRPVSHDSGNDVPTSGLGGSSSDPGSHWNYN
                     QDGSCSDIPNVDVVVPLTSDKAHLIDRISRLEAYGSTGGALGTAMSWYTLSPRWSGIW
                     SSDSRPGAYSDVTTIQPSGNPLLRKVAVLMSDGEYNTFRNWKDQDQVMVSDRAKQICA
                     NMRAAGIEIFTVGFALDQLPTAKRAIAEDTLRSCGGEISHFYSTFNAEQLQMAFRDIA
                     LQVAKLRLTQ"
BASE COUNT         4332 a         8666 c         8812 g         4615 t
ORIGIN      
        1 cagacgggcc gcgcgtgagg tcgagagcgc tgatcagacg ctgcgcccct tctggcggct
       61 cggacgcgcg cggcctctga tcgagggcgc gctctggctg ttgatcggaa tcgccatcgt
      121 cgtgccactg atgtcgctca tcgcgacctc gctcgtcccc gcgctcggcg tgccgctccg
      181 cgcccctacg gcgacgctcg agagtttccg cgaggtgctg ttccgccagg atgtgacacg
      241 gcgggcggcg ctgaattcgc tggtgctttc ggctaccgct gcggtgctgt cggccggtct
      301 cgcgatcgtc gtcgcctacg gcatcgagcg cgtcgcccgt cgctggcgga gcgcgctact
      361 cgtcctcatc gagctgccct acgcgatccc cgggatcgtt ctcgccatcg cctgcatcct
      421 gctgttcctg cggccgctgc cgctcatcgg catctcgctc tatgggacgc cggcgatcat
      481 tctcgtcgcc tacctcgccc ggttccaggc gctcgccttg aagccgacgc tcgccgcgat
      541 ggcgctggtc tcgcgggagc ctgaggaggc tgccgcgctc gagggcgcca ccatgtgtca
      601 gaggctgcgt cacgtggtga tcccgagcct ggcgccggcg gccgtcgcgg gcgggctcct
      661 cgtctttttg cttgctttga acgagctcac cgtctcggcg ctgctgtgga ctgccggcac
      721 cgagacgctc ggcgtcgcgc tgatgagtct cgaggatgcg ggcctcgcga gccaggcggc
      781 agcgctcggc gtggtcgcga ccgtgctcgt cgcgctcatc atgctggcga tcgatcgcat
      841 tgcgcgggga acctcggccg acgccgtgcc ctgggcgagc atttccggct cgcgttgaac
      901 ctcggtcggc cagcgccatt gcatgtgtgc cgtgccgcgc cacgctttgc ctcggcgtga
      961 tgcttctgca taatgcggag ttgatcgcac cggccgagga gcgctgaatg atccgagaat
     1021 tcgtcttcga cgcacccgag ctgccagcgg cggcccagca gctcnnnnnn nnnngagccg
     1081 atgtgcgcgc aacgctcgag gcggagatcg tgcgcggcgc gttcaagccg acggtcgggg
     1141 cgatgacggc ggccgatccg gcgttctcgg agctctgcgg cgccaagggc tggctcggca
     1201 tgacctggcc gaaggcctac ggcggtcacg agcgcacggc gctcgagcgc tacgtcgtcg
     1261 tcgaggagct gctggcggca ggtgcccccg tcatgtcaca ctggatcgct gaccgtcaga
     1321 gcgggccgca gatcctgaag cacggcagcg agcgcgctaa gcgattgatc cttcctgaga
     1381 tcgcggcggg gcgctgctat ttcgcgatcg gcatgagtga gcctgatacc ggctccgacc
     1441 ttgcgagcgt caagacgaag gccgtgcgca ccgacgaggg ctatcgcgtc acaggccgca
     1501 aggtatggac gagctttgcc cacagcgccc actacatcat cgccctcgtc cggaccggcg
     1561 gctcgcccga gcagcgccag gtggggctca cgcagctcat catcgacacg aagtcgccag
     1621 gcgtcggcat ccggccgatc atcaacatta cnnnnnnnnn ngggagcgca cgaattcaac
     1681 gaggtgacat tcgacgacgt gctggtgccg ctcgacatga cggtcggcca ggagggccag
     1741 ggttgggcga tggtgacttc cgagctcgcc ttcgagcgct cggggccgga tcgcttcatg
     1801 tcgaccttcc cgctgctcgt cgagaccttt ggcgcgatgg ggcccgagcc ggctccgcat
     1861 tcggcggccg acctcggccg cctcgtcgcg cacgtggcaa cactccggcg catgtcactg
     1921 tcgattgccg ggatgctgga ggcgggacag tcgccggtca ccgaggcggc gctcgtgaag
     1981 gatctcggca ccacgctcga gcagtcgatc ccggagattg cgcgccgggt gctgccgatc
     2041 gagccgcgcc gcgaggtcgg caacgacgac cggtacatcg agctcctcga actctcgcag
     2101 ctccaggcgc cgtcctattc gctgcgtggt ggcacgcgtg agatcctgcg cggcatgatc
     2161 gcgcgcggcc tcgggctgag gtgagggcac catgagcgag acacgcgaca tgatgcttgc
     2221 ctccttcgac cgggtcgtcg ccgaccatct cgacaggggc gcacgcgagg cggccgacct
     2281 gcagacggca aaggaggcgg gagcggagcc ctggccgtca gaggtctggg cggccctcga
     2341 gcagcaaggg atgacggcgc tcggcgagat cgccgagggt gatctcggct tcgtcgacag
     2401 catggcgctg gtgcgccgct cggcctatca cgcggttccg ctgccgctcg ccgagacgtt
     2461 tgtcgcccgc cgcctcctcg cccgtgccgg cctcgagctt cctgacggcg tactcacgat
     2521 ttccgcacca gcaagcatgc atgacgtcgt ctcggtgcgc gaggggcaga tcacgggccg
     2581 catcgcccat gtgccgttcg cgcgcggcgt ccgccatctc gtgatacccg tcggcagcgg
     2641 gcgcggggcc gcgctcgcac tggtcaatat cgagcgtgcg atagcgcatg cctcgatctc
     2701 gaaggcgggc gagccgcgcg acacggtcga tctaggtcgt gccatgccgg tcgcggtcgg
     2761 gacgatgcac gatgcgccgc tcatcgtcga ggcagagggc gcgctgatgc gggccgtgca
     2821 gatgtcgggc gcgctctccc gcgtgctcgc ggaagcgctc acctgggtca gcgaccgggt
     2881 ccagttcggc aaaccgatcg ctcgcttcca ggcggtacag catctcgtcg ccgagctcgc
     2941 gtcggaatgc gcggcggcga gcgctgcgtc ggacatggcg gtcgaggcgt ccgtcagcgc
     3001 gccggatcgc ttctcgatcg ccgtcgccaa ggcgcgcgtc ggcgaggcgg caggtcgggc
     3061 atcggcaatc gcgcatcagc tgttcggcgc catgggcttc accannnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnaatggga cgccgcactc agatgcacat cctcagttac
     3301 ccatgaagat ctgacggcgc cgcgcctcgt ccgtctgcag gcgcggcttg gcagccctcg
     3361 gatcggcctt cggtggagcg gatgcagggg cggcggtggc tggtgcggcg gcgactgctg
     3421 gcggtggcgg cgccttgggt gccagcgacg ggaccggatg ccgccggccg tgcgcctcga
     3481 ggtcctcgag caggacggcg agcagcggcg aggtcagctg cgccgcgtgc aggttcgtcg
     3541 cgaagggctg gctggtcgag ccggtgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gcaccaggtc
     3721 gaaaggctct tcaaggttcc atgcgcgatg ccgcccgccc aatggcagag cggcgcctgg
     3781 aggaacgcct ttatgagctg atgcgcatgc gggcggatca gtgtgttggg gcggatcgcg
     3841 ctctcggctg agtgcatggc ggcgagcgcg ctctcgcggc tcgtgtagtc gtagctcttg
     3901 acgagcgtcg gcaggcggac cggtttcgcg ccttcgccga tcagcatgtg atgcacacgg
     3961 cgcggcgagc cggagatgag cccgcgaaca gcggccgtcg atggtggcgt cacctcgctc
     4021 gcggacggcg caggcggcat ggcgaagccg agagcatcga tcaggcggcg cagtctctcc
     4081 tgcccgagct gagccgcacg ccactcgatc ggccggttga gcgagcaggc gaaggcgacg
     4141 atggcgcggc ggtggcccgg tgtcgcgccg ctgcctcgct cgcacgtgtc gccgccgcgg
     4201 tcgggcgcga gcgcgtccgg gtagagcgat gagatgacgt cgcgcgcctc gttggcgagc
     4261 gcgatggcga tcaggatctt gcctgtcgag gcgactcggc gctgctcgcg cgccggagcg
     4321 tagaagccgg tcgcggcatc gcgcgcgaag ggcgatccga agtaggccgc cgtctcgccg
     4381 agctcgaagt agcggacgat ctcgccacgg gcatttgcgg caacgacgat gacgttcggg
     4441 atcttgaggg tggcgccggc aacaagcatc gcggggtcaa tcgtgtagcc gggggcaaat
     4501 tttgctccgt gctgcgcgtg gaggcgcttc agctcggcgc ggattttttc cgcgaaggcg
     4561 aggttctcgg cgacatcgag ggtgaccgtg acgccgcgca catagtcgcg ccagccgaag
     4621 ccccagcttt gcttcatctc ctcgcggatg ccgaagcggg cggcgggcag cagcgcgttc
     4681 gcacggataa atgggttggc gacagcgcgg gcagcgagtg ccggagtgtg ggccacgagc
     4741 gtcgcgcgga gcaacggttt cgccttcggg tccggagggc cgccggcgag ctggacaagc
     4801 tcgaagatgg tcgccttctg caattcggga tcgctgacca gcttctcggc gcacgtacgg
     4861 gctcgtacct cggtgatgta gcgccagcgg tcgatgcgca cttcgttgag cttctcatcg
     4921 ccggagagga ggatgatcgg cttgttgacg gcagaagcga gcacgaactg ctcggcgacg
     4981 gtcaggtcct tggcgtcctt gccgaagacg atctgcgagg tgatttcgac gccgtgcagc
     5041 gacgagccgc cggtgcgctg cgcgagccag agatggttgg cggcccaccg cttcaatggt
     5101 gtgtcgtcgc cgtcgcgcgt cagctcgcgg tagatgacgg gggcgagcca ccactcgcgg
     5161 aacttgcgcg atagcttggc gaaaacgccg tggctcgcat ccggcggcgt cttgtcgatg
     5221 acgcgcacga gctgcatcgg cagcgtcgag ccgccgacgc cgagcgaggg acggcgcagc
     5281 gcaatcgagc ggcggatcga ggtgtagggg attttcagga cgccatagag gtcgatcccg
     5341 aacggattga tccaggtgcc gatgttgcgg tcctcgtgga aggcgaggca gcgccaatag
     5401 tgctccggta cctcgttgac ggggacggac ttgtggtcgg ggagggccgt gtagtcgccg
     5461 agctcgatgg cggcgtccgt gtagttcacg tcgcgacggc tgtcgaggcg cgggtcgaag
     5521 ctgccgatga agttgccgcg gttgtcgtag atggcggtcg ccagccagcg ctcggcatca
     5581 tagcggagct tgtcggccag atagtagctg ccgacgtagc cgcggatcgg cgcgacaaca
     5641 tagacgagga cgagcgcgaa ggcgaggtag gcaatcgcgg ccgttgcgac gtggcggagc
     5701 gggccgagct tgcggttgaa cgagaccgcg acgagcagat ggcggacggc atgaaatggc
     5761 agcgcgagga gccgtccgac gagcggcagc aggcgcagcg gcacggcgag cgccagctca
     5821 atcgctctcg agatcagacg taggacggcg agcatgcgcg ttggcctcgg cgcgcgatcg
     5881 gatggggacg ctccccatcc gtcagtcagt tgaggtaggg cgccattgtg gcgccgctcg
     5941 ggaccggagt gtggcgcact tggcgccgcg ctctcccgcg tcgcatcgga agtgcggcta
     6001 cttcagctcg cccttcacct catcgcactt cgggcccatg aagtcgttaa tgtcctcgac
     6061 catgctcgac tgcagcacgc cgagcttgtt gtcggcagca gtcaacgtcc agatggcggt
     6121 cttggcgacg atcggcgcat cgacgtcacc ccagttgagc gaggccttga cggccttgcc
     6181 gcccttgaac gtcaccttcg gctggaatgt gaccttcatc gtgaagggtg tctcgcccgg
     6241 ccgctcgatg tcgcaggtgg cgacatgctt ctcgagtacc atctcgtgct cgccaccctc
     6301 gacggccttc aagaggcccg cgcgcggcag cttgatgtcg gcggcgcatc gggctttgcc
     6361 ccagagccac tgcaggccgc ccttggagac catcttgacg atctgctcct tgcgccacgt
     6421 cttgtagacg ttgcagccga tgtcggggcc gggctttttc agcgcaaaag ccgagcagat
     6481 ctggatcttg cagcccttgc ggtcctcgcg ctccttgagt tcctccggcg tcaactcgat
     6541 cttgggtgcg gcggattgcg accggacgac gggcgtcgcc gagacgagga ggagccccgt
     6601 cgcgacgaga gttgcgccag tgagtgcgaa gacgccggct ttcatgatct gctccctcaa
     6661 tttttcggcg gccgctcggc cgatcgcggg tgaatagcgc ggcggggcga gcaggaatat
     6721 ggcaaggcct cagcgcgtgg cgagcaggcc tgcgaccatc gcgtcctggc ggcggcgtgc
     6781 gattgcgatg gggcgcgtct cgtcgggcgc gacggccgca taagtgagaa gctcgccctt
     6841 cgcgatccgg cgcgtcacct tggcgccttc agcgaggccg accgggagtg cggcccggct
     6901 ttccgcatcc tcgcgcgtca acgtgaaggc gcgataggag tactcaccga tcttgtccag
     6961 cgtctcgccg ggggtgaggt cgcgcttggc aacgcagccg acctcggccg aggggatcgg
     7021 gcgcggccgc atgtggcact cgccgtggat gacgacggcg gcggcagtga gcggcacctc
     7081 gaggctcgtc aggtgatagg gccggaagaa cgcgtagtag ggcccgggcc cgaggtggag
     7141 atctgccatg cgctcgcgca cgcgcgggtg gcgcatctcc gcgaccgcga acacgcccgg
     7201 ggcgacacct ttcgcgaccg tgaaatcgac gcagccgcgg cgggagagga gcccgccgtc
     7261 ctccttcggg cagaaggtgg tctgcaaggc ttcacgcggc gcggcgggtc cgtgcatgcc
     7321 ggggatgtcg ggaacgaggc ctgcggcgtt ggcgattgcg gccatctcga ccatggtctt
     7381 cgagccgtcg acgaactcga ccagcatgcg cgggttcatg ttccggcgcc gtgcctcctc
     7441 gcggtaggcg tccggcgtcg cgtcgatgcg gaaggggttg ttcttgccct tgccggcggc
     7501 gacgaccgga tagccgagcg cgcgcacgaa attgatgagc tcgagggcgg cggtcggctc
     7561 gtcacccgcg ccgagcgaat agatgacgcc ggccttctcg gcttcgctcg cgagataggc
     7621 gccggccgtc acatcggcct cgacgttcat catcacgatg tgcttgcggg cggcgatggc
     7681 tccgagggcg acggcggcgc ccgcgctcgg attgccggtg gcgtcgatca cgacatccac
     7741 ctcgggtgcc gcgaacacga gatcgggctt ctgaacggag gcgaggcgac cgcggccgat
     7801 cgtgcgagag acgtccgcga ggctcgtcac gatctcgggc gtgcgacagg cggcacctgc
     7861 gatcccggcg gcggtggcga tccgctctgg tgtcatgtcg gcggcgacga cgatctcgat
     7921 ccccggcatg gcggcaatcg cgaccatgag gtcggtgccc atctgcccag tgccgacgag
     7981 gccaacgcgc acgggcgtgc cggccgccgc gcgctcggca agcgcctcgg caagcggcat
     8041 ccgtttggct ctcggcgatg gcgggctctc tcgcaggtcg tgcacggttg cctcatctgg
     8101 cgcgagctgg agacgtatcg ggcacgacag gcggcttcaa agccgcagac tgcttaccag
     8161 aacgggctac cggcagggcg gcgtggggtg gtggtgaacg gcgatcctgg cgctggcggc
     8221 gcgccggctg tcggcggggg gaccggcggt ggcgtggcgc cctcgcctcc gaaggctgct
     8281 gcatcggccg tgaagccgat aacctgatgc ggctcgtccg gatcctccat cggcttgaag
     8341 gcttcctcga cgctctccgg cgagcgcggt gccgtcggca gtcctgtctt gagattgacc
     8401 cacaccatct tgaggccggg cgggcggcgg aacggcacgg ccggcttgtc ggcgagcgca
     8461 tacttcatca tgttgccgaa gatcggcgcg gcgacggcgc cgccggtcgc gatctggccc
     8521 atcggacggg gcgtgtcgta gccgacgaat acgccgatga cgaggttcgg cgagtagccg
     8581 acgaaccaga cgtcgcgcga gtcgttggtg gtcccggtct tgccggcgag gcccatcttg
     8641 tcggagatga acgacttgag gatggtcgcc gtgccgcgct ggatcacgcc ttccatcatg
     8701 gcggtgatct ggtaggcggt gtgcgggtcg atgatctgcg ggcgatcgtc cggcacgtcg
     8761 ggctcggcct ggcccgtcca gcgctcggcc ttgcagccga ggcattcgcg ctcgtcgtgg
     8821 cgccagatcg atcggcccca gcgatcctgg atgcggtcaa tgaacgtagg cttcaccttg
     8881 cgtccgccgt tggcgagcat ggcataggcg gccgtcatct tgagaagcgt ggtctcgccc
     8941 gcgccgagcg acatcgagag caccggcatc agatcatcgt agatgccgaa gcggcgcgca
     9001 tactccgtga tgagcggcat gcccatgtcc tgggcgagac gcacggtcat ctggttgcgc
     9061 gacttctcga tgccgaggcg caaggtggag ggaccgtagt acttgccgcc gtaattctgc
     9121 ggggtccaga tctcgtcccc gcggccggtc tcgatggtga tcggagcgtc gagcacgatg
     9181 ctggtcggct tgtagccgtt gtcgatagct gccgcgtaga cgaacggctt gaacgacgag
     9241 cccggctgac gcttcgcctg cacggcgcgg tcgaactggc tcgccgcaaa gctgaagccg
     9301 ccgacggagg cgagcacgcg gcccgtgtgc gggtccatca cgacgatgcc gccgccgacc
     9361 tcggggatct gcatcagcga ccacacgccg gcgggattgg cggggtcctt cggcgagacg
     9421 tagatcacgt caccgacctg gagcacctcg ctcggcgcct tcggctcggc cttcttggcg
     9481 ccgacctttg cccacttcat ctcctcgagc ggcacactgt ggcgctcgat cgcgggcgca
     9541 aaggtgccgt cctggagctt gggcgggcgc aggccgacga tcgccgtctt gggctgcaac
     9601 tcgagcacaa cggaaaggcg ccatggcgtg atgtcgctcg gcacgtcgat ggcgccgagc
     9661 acggggcccc aatcgcccga aacatcgatg ttcttgagcg cgccgcgcca gccgacgtgg
     9721 acgcggtcga aggcgacgag gccgtcgacg agcgctttcc tcgccatggc ctgcatcttc
     9781 gggtcgaggg tagtgcggac ggagaggccg ccattgacgc gcttgtcgcc cgagctgatg
     9841 cgctccatgc ggccgtagag gccatcgtcg ccatagaagc cgacgagctg gcggcgcacc
     9901 tcttccgcga agtagtcggc ggcgtagacg cgggtgccga agggtcggat gttgacggtg
     9961 agcggcttgg ctttcgcggt cttggcttcc tcatccttga tgaagccgtt cttggccatc
    10021 tcgtcgatga tccagttgcg gcggatggtt gcggcctgcg tcttgttgaa ggggtggtag
    10081 ttgctcggcg ccttcggcag cgccgcaaga taggcgacct cctcgatctc gagctcatgc
    10141 aactctttgt tgaagtaggt gaggccggct gcggcgacgc cgtaggcnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnc gcggatcttg cggtcgaagg tacggtcgct cgtcagcagg
    10321 aagttcttcg cgacctgctg cgtgatcgtg gatgcgccct gcaggccgcc gcggcccttg
    10381 atcttcgcct cgacgaactt cacggcggcg cggatgacgc cctggaagtc aatgccgccg
    10441 tgctcgtaga agcgcttgtc ctcggcggag agataagcgt tgatgacacg cttcgggatc
    10501 gagttgatcg gcacgaatat gcgccgctcg cgcgagtatt ccgcgatcag gctgccgtcg
    10561 tgcgcatgaa tgcgcgtcat gacgggcggc tcgtagttgg cgaggctctc gtagctcggc
    10621 aggtcctgcg aggctttcca gatgaggtag ccggcaatcg ccgagccggc gaggaacagc
    10681 accgcgcccg atgcgaaggc gaagccgagg aagctcaaga gccagctccg gcgccgcttc
    10741 ttcttgcggg tcggctgcgc tggcggcggc gggtacgttg gtgctcgcat agactggatc
    10801 gccccaacct ctgttcagcg gcgttctaga ggcgttcgca tgcgccagca agacccgcgg
    10861 aggaaacacg gcaggataac ccggaatatg actttcccgt gccgatcctg tttcaattcc
    10921 gacatatgcg gtcgtcagcc gcggccgatg tggctgagac cacccatgta gggccggagc
    10981 gcctcgggga tggcgaccga cccgtcgccc tcctggtagt tctcgagcac cgcgatcaat
    11041 gttcgtccga ccgcgaggcc ggagccgttg agcgtgtgca cgaagcgcac gtccttggcg
    11101 cctttcgggc gatagcgcgc cagcatgcgc cgcgcctgga agtcgccgca gaccgagcac
    11161 gaggagatct cgcggtaggc atcctggccg ggcagccaca cttcgatgtc gtaggtcttc
    11221 tgcgccgaga agcccatgtc gcccgtgcag agcgtcaccg tgcggaaggg gaggcccagg
    11281 cgcgacagga ttgtttcggc gcaactggtc atgcgctcgt gctcagcgag gctttcctcg
    11341 ggcgtggtga tcgagacgag ctccaccttc gagaactggt gctggcggat catgccgcgc
    11401 gtgtcgcggc cggcagcacc cgcctccgag cggaagcagg gcgtccaggc cgtgacgcgg
    11461 atcggcagct cggcctcgtc gaggattttt tcccgtacga gatttgtgag cggcatctcg
    11521 gcggtcggaa tgagccagaa gccgttggtt gtgccgaaca aatcctcgcg gaacttctcg
    11581 aggctggtca cgccgtgcac gctcggctcg cgcacgagca tgggcggcac cacctcggtg
    11641 tagccgaact cgcccgtgtg gatgtcgagc atgaagttgg cgagcgcccg ttcgagccgg
    11701 gcgagttgcc ccttcaggaa cacgaagcgt gcgcccgaca ccatgccggc cgtctcgaaa
    11761 tccatgaggc ccagggcctc gccgatctca aaatgctgct tcggcttgtt ggtgaagttg
    11821 aaggacttgg gcgtgccgac cttgcgcttc tcgacattgc ccttctcgtc gctgccaacg
    11881 ggtacgtcgg cgagcggcag gttggggatg atcgacaact cgcgctcgag ggcggcggtc
    11941 agctcctttt cgcgcgcctc tccggccgac agctgatcct tgagagtggc cacctcggcc
    12001 atcagcgcct gcgccttggc ctcgtcgcgc gcgctcttcg ccttgccgat ctccttcgac
    12061 gccgcattcc gcctcgcctg cacgtcctgg agcgacgtca catgccggcg gcgctcctcg
    12121 tccatggcga tgagcttcga cgagagggcc gcgaggccgc ggttcttcag gcccttatcg
    12181 aaggcggcgg cattgtcgcg gatccacttg atatcgtgca tgaggcggtc ctcggtgtgc
    12241 ggcgcaacat tgggcgctga cagtggctct ataggacagg cgtcggggca tggtccaccc
    12301 ccgcaatggc gcgccgtgca aggcacgcat gcggcgcggc gaaggcactc ctcaagtgcg
    12361 gccgcggttg ctacaagagc cgcctccccc ttgatcaagg aacctcccat gaccatcaag
    12421 cgccacgagc cctcgcagat cctctcgaag gtcgtcgagg ccaacggctt cgtcttcacg
    12481 gccggcatca cggcggacga tgcaagccag aacccgcagg gccagaccaa acagatcctg
    12541 acgaccatcg acaagctctt gaagatgtgc ggtaccgaca agtcgaaggt cgtctcggcg
    12601 acgatctggg tctccgacat ccgcttccgc gaccagatga acatcgagtg gaatgcctgg
    12661 accggtggca aggatctccc cggccgcgcc tgcatcgagg caaagctcgc cgatccgcgc
    12721 ctcaccgtcg agatcagcgt catcgccgcg aagtgagcgc catgcggatc gaccggcacg
    12781 agccggaggc gggcgtcctc tcgaacgtcg tcggcgccgg cggcttcgtc tacacggcgg
    12841 gctcggtcgc cgacaatctg gacctcgacg tcgagggcca gacccgcgag gcgctcgcct
    12901 acatcgacca gatgctcgcc cgctgcggca ccgacaagtc gaaggtcgtc ttcgcgacca
    12961 tctggctcgc cgacatccgc ctgcgcgatg ccatgaacaa ggcctggctc gcctgggtcg
    13021 acaagggcaa cctgccggcc cgcgcctgca tcgaggcgaa gatggcgaac cccgcctacc
    13081 tcgtcgagat cgcggtcgtc gccgcgcgct gaccgcggcg acggcgcggc tggacaaggc
    13141 acttaagatg aggtcagacc ctcgnnnnnn nnnntcttgt tgggcgaccg atcgttcggt
    13201 tgcctcgcct gtcacgcagt tcaggagacg tgggacgcaa tgtcagagcg catgccgccg
    13261 ggccgccggg ccgactacaa gcatttcctg ccgatcccga cccgctggat ggacaatgac
    13321 gtctacgggc acgtcaacaa cgtgacgtac ctttcctatg cggacaccgt ggtgaaccgg
    13381 tacctgatcg acgtcggtcg gctcgatttc aacgccgatc cggtgattgg catcatggtc
    13441 gagacgatgt gccgcttcca caagtcgatc gcctaccctg acctcatcga ctgcggcctc
    13501 aggatcggca agctcggctc ctcgtcggtg cgctacgagg tcggcatgtt cgtcgccccg
    13561 gacgacgtcg cgcgggccga ggcgcactgc gtccatgtgt tcgtcgagcg ggcgaccaac
    13621 aagccggtgc cgattccgga gaagatcaga tccgcgctcg cccgcatcat gcggggctga
    13681 actcgcaggg cacagcggca tctgcaatgc ggcgacttgc gcaaacgccg cctggccggc
    13741 cctccttaac gcttgcgata cgttgcctcg gcataattgc caggatcgga ccggatgcgc
    13801 cagcaacgcg tccgcgagag agcgttgtcg cgtggagttg agcgtgctcg aagtgcttgt
    13861 atttcttgcg tttccgctgg cgatggcgtt cgccggttcg atggatctct tcacgatgac
    13921 catcccgaac cgcatctcgc tcgcgctcat cgcggcattc gtggtcgcgg caccctactg
    13981 cggcctcacc gtcacccaga tcgcctggca cctcggttgc ggcttcctga tgctggtgat
    14041 cggcatcctg atgttttcgc gaggctggct tggcgggggt gatgccaagc tgctggccgc
    14101 cgcctccctc tggctcggct tcgaacatct cctcggctat ctggtgcttg tggctgtcgc
    14161 cggtggcatg ctggcttcga cgatcatgct ctatcgctcg atgacgatcc cgctctgcct
    14221 catcggtcag ggctgggcga gccgcctcca cgagaagacg agcggcattc cctatgggat
    14281 tgcgctcgct gccgcggcgc tctggatcta tccctcgacg acctggttca aggtgctcgt
    14341 agcctgaacc tcgccgcaag ctttgcttcc ggacgttaag caaaattttc aaacaagtgc
    14401 gaatacgcac cgaaacgggg cgacggcggc atcgccatta acgggccatt gagaattctc
    14461 cagcaaagtg ccttgaacgc gggatcgctc gtggccggtt gaaaggaccc ttgcgagatg
    14521 aaaaaggctc agctcatcgg acttgccatc gctggcgcct gcggcgtcgg cgcgtttctg
    14581 atgatgaaga atgtcgtcaa caagcctcag gtcatccaga aggaggtttc gacgaacctc
    14641 gtcgaggtgc tggtcgcgcg caacgacatc ggcctcggtc aggttacctc cgaatcgcat
    14701 ttccgctggc agccttggcc gcaggacaac atccagccgg gcttcgtcac gcgccgggcc
    14761 ggccagtcga cgagcggtat gaaggactac accgggcgga tcgcccgcgc tccgatcatg
    14821 ggcggcgagc cgatcacggc gatcaagctc atcaaggcgg gtgagggcgg cgttctcgcc
    14881 tcgatcctcc ccgaaggcta tcgtgccatc tcggcgaaga tcacggacga tacggccgtc
    14941 ggcaagatga tcctgccgaa cgaccacgtc gacgtcatcc tgatccggcg cctgcgcggc
    15001 cgcggcggcg ccgaggagca cgtctccgac acgctcttcc gcaacatccg cgtgctcgcc
    15061 atcggccagg tcatccaggt cggcgatccg agcaagaagg gtgctgatgg tacgacggcg
    15121 acactgcagc tcacatcgcg ccaggccgaa ctcctggcac tcgcgaaatc gatgggcgag
    15181 atcagcctcg tgctgcgctc cgtcgccgat ttcaagaagg acgccgatgg caacgccgcg
    15241 gacgcggaga acctcaagtc gcagcgcggc aatgcagtgc gcgtgctccg ctacggcgtg
    15301 cgcaaacgtg attacggcgt gaactgaggc agggcgacgc ctgcctcccc gtccgccgct
    15361 ctaagcatga cccaagagtc cagtcgtcag ttcctttgtc tcgcgtcggt tgcgtctgag
    15421 tgcacccttg tcggtagctc gtcagaaggc ttgtcgaacc tatgcggaag acattgaaca
    15481 gcgcctggat cccgggcctc gtggggctgg cgggcatcgc cgccctcgca ttcgcgccgg
    15541 gcctcggctc gtccgccctc gccaacggac cgaagcgcgg cgcgccgatg ccgggcacgg
    15601 tcgcctcgtt tgcccccgag caccagtcga cgctccgcat ccgcgagagc gaccggcttc
    15661 ccctgcacaa aggcgtgcgc ctcgggcgca acaaatcgat gctggtcgag ctgccacggg
    15721 agcttnnnnn nnnnnnnnnn nnnnnnnnnn ngcggtcgtg cagagctcga accgggtcta
    15781 cctgatcggc aagaagaccg gccaggccaa cgtcttcttc tttgatgcca acggcgagca
    15841 gatcgtcacc ctcgaggtct cgatcgagca cgacacggcc gtcatggagg cgatgttcac
    15901 ccgcctcatt ccgggctcgc gcatcaaggc cgagatgctc aacgagacgg tgatcctcac
    15961 cggtcatgtg cgcaatcccg ccgacagcgc ccgcgcctcc gatatcgcct cgcgcttcgt
    16021 cgtgacgcct tcgccggaag ccaacggtaa gcacaaggac aaggtcatca acctgctcgc
    16081 cgtcgaaggc gaggagcagg tgatgctgaa ggtcgtggtt gccgaagtgc agcgctcgat
    16141 cctgaagcag ttcggcatca atattggcgc cgagctcaac gtcggcaaca tggcgctggg
    16201 tctgctcacc gccaacggcc tgccgctcac ctccgccgca ggtctcggcc gtttgccggt
    16261 tgcaggctcc gatctcgctt cgcccatggg ccaaggctgc ggcagcgtct tcgatctcgc
    16321 caagacgggc gtcatcggcg gctcggggcc ttacggcggc tggggtagcg gcaaccactg
    16381 cgtctcgcac gtgatgcgcg ccctcgagcg tgacggcctc gtgcgcaccc tcgccgagcc
    16441 gaacctcacg gcgatctcgg gcgagcccgc gaagttcctg gccggtggcg agtatccggt
    16501 gcctgttgtg gacagcctcg gcaagctctc tgtgaccttc aaagagttcg gcatcggcgt
    16561 gaccttcacc ccggtcgtgc tctcggaagg ccgcatcaac ctgaagatcg aggccgaggt
    16621 ctccgagctc accgacgccg gctccgtgac gctctcctcg atctcgatcc cggccttgaa
    16681 gaagcgacag gcgaagtcgg tggtcgagct tccctcgggt ggcacgctcg ccctcgccgg
    16741 gctcatctcg gacgatacgc gccagaacat agacggtctg cccggcctca aggacgtggc
    16801 gatcctcggc acgctcttcc gcagccgtga cttcatcaag cgcgagaccg aactcgtcat
    16861 catggtcacg ccctacatgg tgaagcccgg ccgccggcag gacttcgccc gccccgacga
    16921 caatctggcg cccgcgtccg acatcaaggc caacctgctc ggtcacctca atcgcgtcta
    16981 cggccgctcc tccgcgatgc cggccggcga tctgaagggc gactacggct tcatcgtcga
    17041 ttgatccgag gcaaggaaac gtatcatgac tgacattctc cacatgcctc cgagcaccac
    17101 ggcgagcaag cgcccgctgg tcaaagtcgg cgcgtcaatg attgccgcta tggcactcac
    17161 agtgacggtc gccggctgca agcacggcga ggagccctcc ggccacgtcg ccggctggac
    17221 gctcgtcgat ccgagccacc gccacccgat cgtcgtccac cagaagccga cgacgatcgc
    17281 ggtccgagtg gcgcgcggtg ctgatggcct caatccgcat cagcgctcgc aggtcgtgag
    17341 ctttctcgag cgctaccgcg gcgtcggcgc gggcaacagc cgcctggtga tctcggttcc
    17401 ctcgggcgcg ccgaacgagg tctcggcgat gtcggccgtc accgacatcc gtcatctcgc
    17461 ccgcgatctc ggcttcgatg aaagcgtgat cggggtcgag gcctaccaca tggagggcga
    17521 caacgccccg ccggtgaagc tctcctacac gcacttcgtc gccgaggccc cgcactgcgg
    17581 ccagtggccg acgaacctcg ccgaggaccc gcgcaatgtg ccctatccga acttcgggtg
    17641 cgcgacgcag cgcaactttg cggtgatgat cgcgaacccg gccgatctcg tcggtccccg
    17701 cggcatgacc ccgcgctccg ccgagcgtgg cgatcaggtc tgggaacgat ttgtaaaggg
    17761 tgaatcgacg atcaccaaga aagacaatga cgagaagaac gtcgtcaaag gcgcgaactg
    17821 acgagggctc atatgtctag gcaagccaaa attcagacgg caccggccga cgagccgggc
    17881 gggcgcgaac tcgcggtgat ggaggagcca ggtttccccg tcgccgccgg ctcggagaag
    17941 gcccgtccca tcccgcgcat ctcgatccag gccttctgcg agtcgagccg cacggccgag
    18001 gcgctgcagg tcgcggcgga ggaccggcgc ctcgccaagg cgcacgtctc ggtgcacatg
    18061 ggtggcgtgc aggcagccgt tgcccactac caggaaagcc cgacgcccaa tctcatcgtg
    18121 ctcgacagcg cgcttccccg cgcggagctg ctcggcgaga tcgaccggct cgccgaaagc
    18181 tgcgactccg ggacaaaggt cgtcgtcgtc ggtcatacca acgacgtcgt cctctaccgc
    18241 gagttgctga agcggggcgt cagcgagtac ctgatcgcac ccatctctcc gatccagttc
    18301 atggaatcgg tctcgaacct ctacaacgat ccggacgccg atccggtcgg gcacgtgttt
    18361 gccttcatcg gcgccaaggg cggcgtcggc tcgagcacca tctgccacaa cgccggttgg
    18421 gcgatctcgg agatattgca gaccgagctt gtcattgccg acatggacct gccgttcggc
    18481 acggccggcc tcgacttcga caaggacccg gtgcagggca tcgcggaagc gatctcgagc
    18541 ccggagcgcc tcgacgaggt gctgctcgac cgcctgctga cgaagtgctc cgagaagctc
    18601 tcgctgtttt cggcaccggg caccgtcgat cgcgactggg acctcgcgcc ggacgcctgc
    18661 gagatggtgc tcgacgtggt gcgccagcac atcccccacg tcgtgctcga cctgccgcac
    18721 gcctggaacg gctgggtgcg ccgcatgctg ctcgccgccg acgagatcgt catcacggcc
    18781 gtgccggatc tggccaacct gcgtaacgcc aagaacatcg tcgatcacct gaagggggcc
    18841 cgcaacaacg acggaccgcc gcatctcgtg atcaacatgc acaacacgcc gaagcggccc
    18901 gagatcccga tcaaggagtt cgcccaggcg ctcgatctca acccgatcgc cgtcatcgag
    18961 ttcgatgccg agacgttcgg cgaggccgcc aaccacggcc ggatgatcga ggagctcaat
    19021 gcgaaggcga aggcggtgca accgctccgc gacatcgccc tcacgctgac caagcgtccg
    19081 ctgccgaagg tggagaagaa atctccgctc gccccgctcc tcgagaagct gaagctcaag
    19141 cgctaaggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggct cctgctgcgt cgcctgcggc
    19261 tcccaagccg caggaggcga aggcggcagc accgacggaa gcacgggcac cgaagccgcc
    19321 ggctcccgag cccgagccgc aaaggaagca ctcggaggag tactacgacg tcaagacgac
    19381 ggtcttcaac gcgctgatcg atacgatcga cctcacgcag ctcgccaagc tcgacgcggc
    19441 agctgcgcgc gaggagatcc gggacattgt ctcggagatc atccagctga agaacgtggt
    19501 gatgtcgatc gcggagcagg aggagctcct cgaggacatc tgcaacgacg tgctcggcta
    19561 cggcccgctc gagccgctgc tcgcccgcga cgacatcgcc gacatcatgg tcaacggtgc
    19621 cggcacgacc ttcatcgagg tcggcggcaa ggtgcagaag acgggcgtgc gcttcgctga
    19681 caacgcgcag ctcatgaaca tctgccagcg catcgtgagc caggtcggcc gccgcgtcga
    19741 tgaaagctcg ccgatctgcg acgcccgtct cccggacggc tcacgcgtca acgtgatcgc
    19801 acccccgctc gccatcgacg gcgccgcgct caccattcga aagttcaaga aggaccgcct
    19861 gcgcctcgac aacctcgtga agttcggctc gatcacgccc tccgtgaagg cgatcctcga
    19921 ggtgatcgga cgggttcgct gcaacgtcct catctcgggc ggcacgggct cgggcaagac
    19981 gactctgctc aactgcctca caggctgcat cgaccacgac gaacgcatca tcacctgcga
    20041 ggactcggcc gagcttcaat tgcagcagcc ccacgtggtt cgcctcgaga cgcgcccgcc
    20101 gaacctcgaa ggcgagggcg agatcaccat gcgtgacctc gtcaagaact gccttcgtat
    20161 gcggcctgag cggatcatcg tcggcgaggt ccgcggaccg gaagccttcg acctgctgca
    20221 ggccatgaat accggccacg acggctcgat gggcacgctc cactcgaaca gcccgcgcga
    20281 agccatcagc cgtctcgaat ccatgatcat gatgggtggt ttcgcgctcc ccgtgaagac
    20341 catccgcgag atgatcgtcg gctcgatcga catcatcatc caggcctcgc gccttcgcga
    20401 cggctcgcgc aaggtcacgc acgtgaccga ggtggtcggc atggaaggcg aggtcgtcac
    20461 gctgcagaac ctcatcgtct acgagatcac gggcgaggat gcgcacggca agctgatcgg
    20521 ccgccaccgc tcgacgggca ttgcgcgccc gcgcttctgg gatcgggccg agtactacaa
    20581 ggacaccgac aagctcgcca tcgcgctctc cgattccgag gtccgcgaca gcaacggcaa
    20641 cccgatggga gaaagcaatg cttgacggac agaacctcca gctgatcctc ggtgtcgtga
    20701 tcgcgctctc ggtcggcggc atcatctacg ttctcgccta tccctatctc tcgggcgagg
    20761 tgcagactga caagcgcatc caaggcgtta ccgaggcaaa gtcgcgccgc tcgcagcgca
    20821 aaggcagtca ggccgacatc gcctcgacca agcgcaagca ggtagccgat acgctgaagg
    20881 agctcgagga acggcagaag gcgaaagaca agatctcgat gaagctgcgc ctgcagcgcg
    20941 cgggcctcga tgcgacgccg tcgatgttct ggatcgcctc ggccatctgc ggcgccgttt
    21001 gcggcgccgg agcctttttc gcgtttccgt cgatgccccc ttacgtggct ggtgctgccg
    21061 ccttcgtcgg cgcgctcggc ctgccgcgct ggatcctgat gcagatgacc aaatggcgcc
    21121 aggccaagtt cctgaacgaa tttgccaacg cgatcgacat catcgttcgc ggcgtcaagt
    21181 cgggcctgcc gctcaacgaa tgcctcggca tcatctcgcg cgagagccct tcgccgatca
    21241 aggaggagtt ccaggagatc gtcgagcagc agcgcgtcgg cgttccactg gtcgactgct
    21301 tcgatcgaat gatgcagcgc attccgctcg ccgaggtgaa gttcttcgcg atcgtgctcg
    21361 gcattcaggc gcaggccggt ggcaaccttt ccgaggccct cggcaacctc tcgggcgtgc
    21421 tgcgcgaccg caagaagatg cagggtaagg tgaaggcgct gtccgccgag gctaaggcct
    21481 ccgccatggt gctcggtgcc ctgcccttca tcgtcatgac gatggtctac gtcacgaccc
    21541 ccgactacat cgcgctgctc ttcacgaaga agatgggcca gttcctgctc gtcgcggcgg
    21601 cggtgtggat gtccgccggc attttcatca tgcgcaagat gatcaacttc aagttctgac
    21661 gctgtttcag cgcgcccggg gcagagccgg ggcagctctc acaaggcaag tgccatggtc
    21721 gatctcatcg aaaagcttct gcagccgcag ctcgtcgtga tggtgctggc cgccattgct
    21781 acgttcgcga cggtgctgac gatcgtgctg ccggcgctgt ccggtgatcg catgacgcag
    21841 cgcatgaagg tgatggcggt cgagcgcgac aagatgcggg cggcacgcct ctccgatctc
    21901 aacaaggaag ggcagggcaa gctccggcag gcgccgaagg gcttcatgca gcagatcgtc
    21961 aaccagttca acctgcgtca gaccttcgag accgacgaga tcaagaacac cttgaagatg
    22021 gcgggcttgc gtggccaggc gccgctcgtc gcctacatgt tcttccgtct cgtcgcgccg
    22081 atcatcgtga ccatcggcgc gctcgcctat ctcttcttca tcggcaacat ccagtatccg
    22141 ccgatcgcaa agttcggcat ggccatcagc gccggctacc tcggcttcta cctgccgaac
    22201 atgttccttt cgaaccttgt gcagcgccgc cagcagtcta tcaactcggc cttcccggac
    22261 agcctcgaca tgctgctcat ctgcgtgcag tcgggcatgt cggtcgaagc ggccttcggg
    22321 cgcgtcagca aggaggtcgc gagccagtcg ctcgagctcg ccgaggagtt cagcctgacg
    22381 acggccgaac tctcctatct ccaggatcgc cgcgtcgcct tcgagaatct cggcaagcgc
    22441 accattcccg gcatcaagtc ggtcgcgacg gccctcatcc aggccgagcg ctacggtacg
    22501 cccgtcagcc aggcgctgcg cgtcatggcc aaggaaaacc gcgacatgcg catgctcgag
    22561 gccgagaaga aggcagcggc cctgccgccg aagctcacgg tgccgatgat cgtattcttc
    22621 ctgccggtgc tgttcgtcgt gattctaggc cccgcggtca tccaggtcat gaagtggacc
    22681 tgacgccgct tggacgaacc tggtcgcacg cgagccgccg gtcatgtgcc ggcggctttt
    22741 ctgtctcgcg gacggcttct cagccgcctt ccgcgcgtag cggccgtgac gagcagcaga
    22801 actcgccgag gcccgcgacc tcgatcttgc cgccgacgaa caggcagaga tggcgtgcca
    22861 acatggcggc aagcgcaagg cgctgctgta tctcgggatg gtccggctcg gcgatgatca
    22921 gattgtagtg gtaggcgagc tcgcgcgccg tctcgcggca ggcgttgaac gaggggtcgc
    22981 ccttggcgcc ttggcggtgc agcgccagga ggcggaaacc ttcgcggcgc tcctcggtcg
    23041 gggtctgccc cctggtctcg atccagattt cgaggacgcg ctcgccaagc gcaagcagct
    23101 tttcgactgt cgcaacggcc gcgccgtggt cgtacgcgtc gcccccactt gccagtgcct
    23161 caatctcggc ctcgaccgcg gccatggcct ggctggccgt tcttgtggcg atttcaggca
    23221 tcatcatctt tgcgatccac gtcctgacgg accttgggag aggcgagcag cttctcgatc
    23281 cgcagcacct cgtcgaaggt atcatccgcg cggaaacgga tgtcgggcgc gaacttcagg
    23341 ttgacggcct gggcaaccac gccccggatg aagcgcttgt tgcgctcgag cgcggcgatg
    23401 atgggcttca ggtccttgcc gccgagtggc atcacgtaga tcgtcgcggt ccgcaagtcc
    23461 ggcgacatgc gcacctcggg aatcgtgatc acggcgccgg tgatgacagg atcgtggatt
    23521 tcgcccctcg ccagcatctc ggcgagcttg tggcgcatca gctcgccgac acgcagctgg
    23581 cgctgcgagc cgccgccctg gcgggtgtct cgggtctgtt tctgtcgcga catggcttcg
    23641 tgtccgacaa aatcgccaat cggtcaatcg gcaagtgccc cggaaaagcg ggtctgcaac
    23701 cggtccgcgc ggcatgcgct caaatggcat ttgccggcct gtgccggcta gcactgacgc
    23761 cgttcctccg tcagaatagc tctgatcgcg gccctctata tcggccgata acggaacact
    23821 gccatgcctg cgagcctcaa gcctgttcaa gtcgctgggc cctggaagaa cactcagggc
    23881 ggcatggata cgatcaacga gaccttcacg gagtccaccg atgcatggtg agcggctgat
    23941 gacggcgcaa ttgccgcgat atccgactga gcgttgcggg gcttttcgat gcgggaggca
    24001 ggtccaatgc tcttcgagcc tctgacgatc aacggcatca ccttcaagaa ccggatcgtg
    24061 cgctcctcac tcggcggtcg atcatcctac tacgatggca cgcccagccc ggcatgggtc
    24121 ggcttcgagc gccgtttcgc gaagacgggt gtcgcgagca tcatctcggc gaccatcggc
    24181 atcaatgcgc ggcgcatgtc gccgcacgag tatccctcga tcgcgcacga tcgcttcatt
    24241 ccggccttcc gcacgggcgt gcgcgcagtg caggcggagg gctgccgcta catcatgcag
    24301 atcggcgatc cgggcggcca cacgcaggtc ggtcttttct cggaggaggc ggattcgaag
    24361 tcctcctcgg ccggcttcga tttcgtctac ggctatcgca atcgcctgac acccatgacg
    24421 atatcggaga tcgaggcctc ggtccgcgaa ttcgcgagtg ccgcgcgccg cgtccgcgag
    24481 atcggctgtg atggcctcga gatcacggcc tcgaaaggct atctgatcca ccagttcctc
    24541 aatcctggca tcaatcgccg caaagatgag tacggcggca gcgacgagaa ccggttccgc
    24601 ttcctgcgag agatcgtcgt tgcggtacgc cgcgagatcg ggcgggactt cctgttcggt
    24661 gtgcggctgg ccgcggaaga tctcaattcc tcgcccgtca acctccgctt gccgatcgcc
    24721 tggccgctgc gccaccactt ccgcggcaac ggcctcgccg agacgacccg gcacgcgcgc
    24781 tccctcgagg cgctcggcgt cgactatctc cacatcgatg ccggctacgg atttcctcac
    24841 ccccacgtga acctcggcaa atggccgctc gagggtttcc gcctctacgc gaattccgtg
    24901 cgccacctga gcctcaagtc tcgggcccgc gcagtcgcgt tgaacctcct gccgggtccg
    24961 cttgcgcggc tcgccttcgg cgccggctgg aacaagaagc cgggtgccaa tgcgcgctat
    25021 gccgaggtct tcaagaagac cgtgaagctc cccgtgatcg ccaacggcgg tttccagtca
    25081 cggcggctca tcgaagagac gctcacttcg ggcaaagccg atctcgtcgc tgtcggccgg
    25141 ccgttgctcg ccaatccggg tctcatcgat gcgtttcgcg acggccgcga cgaaccggat
    25201 cggccctgca cgttctgcgg cgagtgctgt gccaagaccg cgaccttgcc gctcggatgc
    25261 tatgaccgca gccgcttcgc ctcggaccga gacatggagg ccgagatcat gcgctggtcg
    25321 gcgtcaccgc ccgccgacga cgagggtcat ctggcggtgg aattgccgtc cgcggccaag
    25381 taggcaggtg ggggtccatc cgactactgc gtcaggcgga gctttgccac ctgcagggcg
    25441 atgtcgcgga aggccatctg cagctgctcg gcgttgaagg tcgagtagaa gtgggagatt
    25501 tcgccgccgc aagagcgcag cgtgtcctcg gcgatggcgc gtttcgcggt cggcagctgg
    25561 tctagcgcaa agcccaccgt gaagatctcg atgccggccg cgcgcatgtt ggcgcagatc
    25621 tgcttggcgc ggtcggagac catcacctga tcctggtcct tccagttgcg gaaggtgttg
    25681 tattcgccgt ccgacatcag cacggcaacc ttgcgcaaaa gcggattgcc gctcggttgg
    25741 atggtcgtca cgtccgagta ggcgccgggg cggctgtcgc tgctccagat gccggaccag
    25801 cgcggcgaca gcgtgtacca gctcatggcc gtgccgagcg cgccgccggt cgagccgtag
    25861 gcctcgagcc ggctgatcct gtcgatcagg tgcgccttgt cggacgtcag tggcacgacg
    25921 acatcgacgt tcggaatgtc gctgcagcta ccgtcctgat tgtagttcca atgcgacccg
    25981 ggatcgctgc tcgatccgcc gaggccggag gtcggcacgt cgttgccgct gtcatggctc
    26041 accggacggc gcgagccgtc gtgagcgttg agccaggtga agggtccggg cgcagcatcc
    26101 gtgaattctt cgggccccgt gcggtcggtg acgcacggca tgatccgcca gccggaatag
    26161 ttgttggtct ccgtccgcgt gcaggcccac gttccattgg tttcgctgcc gccgctgccg
    26221 cttccctccg tgcagaacgt gtaccaaccg gaccatgtgg cctccatgtt ggtgaggttg
    26281 cgcatcagcg tgtcgccgtc gccattgggc gccatccgca cgcgcgtcga gaacgggacg
    26341 agcgctaccc gcgacgtgta gcggctctgg tccgaccaga cgacgatgtt gacgaggtcg
    26401 gttgcggcgg cccgaagtgc gttgagtttc gtgccgctcg agcaggggcc gacaccgtcg
    26461 tcgcacatcg agcctgtgac atcgagcatc agtgcgagct cgacgttcgt gccgctgctg
    26521 ccgccggaag ccaggtcggc cctcgcggcg atgttgatcg gcaactcgcc gatgccgatg
    26581 aggttgagga aggtcgtgcc gacggccgcg tcgacaatgc cgacgatctg gctgctattg
    26641 cggatctcga aggtcgcgcc gtcgcgggtc aagccaaccg ggcgatttcg cgagtagaag
    26701 cgttcagcga cggcgatcac ctcgctgttg ctcagtccga gctgcactgc gcgacccgat
    26761 gccagcaccg cggaatcgat cgagccttgt attctgttat aggcggcgtg ccagcgccca
    26821 tagtctatgg caccgcccgc caacacgacc aggaccgtca tgacgatggc ggcgatgatc
    26881 gccatcgagc catggcaatc gtcacggaac tcggtgcccc gctgccacaa acctgccggt
    26941 gcgcatggac ccactgatcg
//
LOCUS       DAORNH010000021        26627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1395_length_26627_cov_2.564466, whole genome shotgun sequence.
ACCESSION   DAORNH010000021 DAORNH010000000
VERSION     DAORNH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26627
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1395_length_26627_cov_2.564466"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>245)
                     /locus_tag="PK264_03905"
     CDS             complement(<1..>245)
                     /locus_tag="PK264_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75073.1"
                     /translation="AMTSIMATARLFAERMKGAVSGVALRPMHVEIVAPDPIVAVTFP
                     PADWDDGEFSAKARKAFESAAQGSSFRWRAGPSVDDT"
     assembly_gap    246..255
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(447..1220)
                     /gene="bcsS"
                     /locus_tag="PK264_03910"
     CDS             complement(447..1220)
                     /gene="bcsS"
                     /locus_tag="PK264_03910"
                     /inference="COORDINATES: protein motif:HMM:NF028346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HRD75074.1"
                     /translation="MRRVVRSVLLAGAVFAPLLGVAREASAQSADPSTFKMLPWREIW
                     AGTDVMGHAWSAYVGQTFSLSEGIQADGWRIRAVSGYGRYTYVRDHWAGSASTPLRHH
                     GVSSFADILLGYHANMGALTVKAFAGAHLDRYDVTPADPDFDLAGYGTGAKLVLESWL
                     NIGSAGFASLDLAWTTGRDTADARLRTGWRALPALSIGLAGQANGDLALQSLRAGAFV
                     RYEWASGEVSASAGAAETRGDNLRRDDGLYGTLNVLFRY"
     gene            complement(1336..2022)
                     /locus_tag="PK264_03915"
     CDS             complement(1336..2022)
                     /locus_tag="PK264_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRD75075.1"
                     /translation="MRIVVSFAAVLAVALAAAPASAADLGAPRPVRASEPYYDLTRPE
                     IFHPRWHGFYLGGTLGLGFGTTAADGGLGNFSFDTEGGLGTVFMGYNWQVTRGIVLGL
                     EADIGTGGLGGSAATPFGTVTSEVNALGSFRGRAGVVLGPALMIYATAGLAWADLDLQ
                     LAGTTSRGETFFGYQLGAGADLAISDHVGLRLEYIYTDLGAERIIHGGTATNYDPDFH
                     TLRAGITFKF"
     assembly_gap    2394..2415
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2416..3586
                     /locus_tag="PK264_03920"
     CDS             <2416..3586
                     /locus_tag="PK264_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRD75076.1"
                     /translation="KGGVNRLTLTDLDRQSRDLFIRWCKDAGCTLKVDQIGSLFARRK
                     GSEDSLPPVMVGSHLDTQPTGGKFDGALGVMAALEVVRTLNDLDVRTKHPIEICMWTN
                     EEGSRFAPAMTASGVFAGVLKLEDALKIKDVDGKEIGAELARIGYAGKEPVGRRPVHA
                     LFELHIEQGPILEEEKIDIGIVTAANGQKWYEITLDGVESHAGPTPMNRRKDALVGAA
                     RIIDLVNKIGHAHDPAACATCGMIQAYPNSRNVIPGRVFLTVDFRHPDAAKLASMDRE
                     LRRGIEEIVAKAGLKVDMKMVADYAPQPFDPACVDAVRKAAKRLGLSAREITSGAGHD
                     AVYMARICPTAMIFTPCVDGISHNEAEDMTPAWAEAGCNVLAHAVLEKAEIVGAR"
     gene            3624..4856
                     /locus_tag="PK264_03925"
     CDS             3624..4856
                     /locus_tag="PK264_03925"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD75077.1"
                     /translation="MSKLQRRGPLVGMRVLDLSHVMAGPTCGRMLADMGADVIKVEKA
                     DGGDDTRKMLPPDIKGESAAYMMMNRNKRGIVLDLKKPAAREALLALVKAADVLIENY
                     RHDTLDKLGLGYEALAKVNPGLIYCAISGFGRSGPFSEQGGYDLIAQGMSGLMAITGE
                     APGRQPVKVGSPACDITAGLLGAMGVCAAYASKLKTGKGQIVDTSLFEAGITLTYWQS
                     AIAFATGVSPGPMGSAHPLNAPYQAFETKDGWMNVGASNQKMYPMLCELLGAPGLARD
                     PRFVTGRDRMNNLAVLIETLAPYFKKRTTADWLVDLERLGIPAGPVLSIREMHAHPQT
                     LARDMVPAVEHPIAGAVQTIGLPVKFSGTPGEVLHAAPLFGEHTREVLVEAGLAAAEI
                     DRLIKEGAAVETRRTAKA"
     gene            5101..5852
                     /locus_tag="PK264_03930"
                     /pseudo
     CDS             5101..5852
                     /locus_tag="PK264_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5860..7311)
                     /locus_tag="PK264_03935"
     CDS             complement(5860..7311)
                     /locus_tag="PK264_03935"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HRD75078.1"
                     /translation="MTSTPIILWYRQDLRIRDHRALTAAVQADVPIVPVYILDDETPG
                     PWRMGGASRWWLHHSLEQLQASLKALGSKLILRRGDTLDVMSDLIRETGARAIHCTRC
                     YEPWAGTLERSLNERLSGDGVALKRYGGGLLYEPEVLKTHHGEPFKVFTPFWRAMLSL
                     DPPRLPIMPPAAIKGPSRWPSSERLTSWHLLPKKPDWATGLKAAWHPGEAGALKQLER
                     FLDMALAGYAEDRDRPDLPGTSRLSPHIHFGELSPCQCWHAVAARAAVEPSAANGASS
                     FQRELAWREFSVNLLHTFPTLPEQPFRPEFAEFEWTSDPQALAAWRKGRTGYPIVDAG
                     MRELWATGYMHNRVRMVVASFLVKDLLVSWKDGEAWFWDTLVDADLANNAASWQWVAG
                     CGADAAPYFRIFNPVKQGQTFDPNGDYVRKWVPELAELSDAHIHAPWLATDEILARAN
                     VKLGETYPIPIIEHRDARDRALATFAKLRGVRG"
     gene            complement(7442..9205)
                     /locus_tag="PK264_03940"
     CDS             complement(7442..9205)
                     /locus_tag="PK264_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD75079.1"
                     /translation="MMALRALVATLMALALCWTAEAAGDAAAAKMAEAARALQEGKMQ
                     SAADLYSEALKDPTLPNDRRAAILVDRAVAFAALGQTRAALDDFNRATQLYPEYAALY
                     NNRGTVLASLGLLKEAVKDFDRAIVLSPAYAAAHNNRAGVFLKLDAPDEALRDYGRAI
                     ELMPTSPLPYLGRAQVHLKLDRPFAAVRDLGRAVAIDARFSAAYRARAEAKMRLERFE
                     EAIEDLSRATAFEPQNVENYMKRGYAYLAARNNVGALKDFTRATELAPKSAAAITARG
                     FGHAKAEAYDEALNDFARGIELDPRSPVTFAYRAWAYRQSGQAELGLKDIERALKLDP
                     ERAEVLWARGEIRESLGQKEEAIADLRKAVALRPDLRDAVLALERLGVPPIAEETAVA
                     DLGIDEWRVHVVGRRLFAMSSRMARLQVPLEVVGSGRPKLLHWDVTTPPPGYGVLTFE
                     AGGVERGGSREEHVGFAVIDLQGRAILALDLLKSGARSATLAWTPTRLTITGLDGQTE
                     EVALRSASSALAPPPVAGPGGPKRALSAVDAPPSTAKAKGPPAWAPWADNPTYARPDR
                     APPKSPSKSGKPKSWFESLFN"
     gene            complement(9353..9835)
                     /gene="nusB"
                     /locus_tag="PK264_03945"
     CDS             complement(9353..9835)
                     /gene="nusB"
                     /locus_tag="PK264_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRD75080.1"
                     /translation="MTGASEGGLTARRAARLAAVQALYQMDVAGTDLNDVIGEFLSYR
                     LTPDAEDQTLAHADRQLFADTLRGVVRRQREIDPIIDRQLADGWRLTRIDAILRATLR
                     AGVWELIERTDTPLKVVITEYLEVARAFLDDEGPKVVNGILDKLARQLRKPADGPAKG
                     "
     gene            complement(9832..10341)
                     /gene="ribH"
                     /locus_tag="PK264_03950"
     CDS             complement(9832..10341)
                     /gene="ribH"
                     /locus_tag="PK264_03950"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRD75081.1"
                     /translation="MAGTSTSASGPSRRIGGLRIAIIEARFYEDIADEMAAGALAVIE
                     ADGSHAERFSVPGALELPQLAQIVAACGRTGTSARTARFDGIVALGCVIRGETSHYDI
                     VCNNANHFLYEVARAFTIPIGNAILTVDTREQALARARGGIAGKGGDAARACLRLIEI
                     AHDLHGRPQ"
     gene            complement(10346..11494)
                     /gene="ribB"
                     /locus_tag="PK264_03955"
     CDS             complement(10346..11494)
                     /gene="ribB"
                     /locus_tag="PK264_03955"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HRD75082.1"
                     /translation="MGGLGTAVYAEGKGFISPIEDILEDFRNGRMIILVDAEDRENEG
                     DLIVPAQMATPDAINFMAKHGRGLICLSLPQERANELRLEAMARNNEARNRTAFTVSI
                     EAREGISTGISAADRARTISTAIDPTKDHRAIASPGHVFPLVAREGGTLVRAGHTEAS
                     VDLARLAGLYPAGVICEIMNDDGTMARMPDLVAFAQRHGLKIGTIEDLIAYRLKNDRI
                     VKCTTRTTVESAFAGSLELHVYETAVEPAEHLALVKGDLKAPGPVLVRVHAINMPADV
                     LGIGATSGSGNLIEKALAAIQAEGRGVLVLLRDLRPKSVSSWIKAAATSGGRAKTQER
                     TLVELGIGSQILRDLGVSEMTLLTNSPDTHYVGVEGHGLRIVGTRKLG"
     gene            complement(11518..12081)
                     /locus_tag="PK264_03960"
     CDS             complement(11518..12081)
                     /locus_tag="PK264_03960"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRD75083.1"
                     /translation="MVEERTGGRFAIRSRYPADGIAIGASIACDGCCLTVTRLTPVGA
                     GSIFAVDVSNETLSKTTLGSWAAGRHVNLERALRAGDEFGGHVVSGHVDAVARIAAMH
                     DDGMSRRFTLEVPAELARYIAPKGSVALDGTSLTVNEVEGHRFGINLIPHTLTVTSWG
                     AKKPGDLVNLEVDIFARYVARFMEASR"
     gene            complement(12142..>12531)
                     /locus_tag="PK264_03965"
     CDS             complement(12142..>12531)
                     /locus_tag="PK264_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75084.1"
                     /translation="NAARDALVGSPAGRIVFVSSFVAHRFVAGANFPASAAAKAGGEA
                     LARALAIELAPHQVTVNSVVPGYTRKDHGAGVLGTAAWDAAARLTPLGRIADPADVAA
                     LIAFLLSDEARHITGQSIAVDGGLSLA"
     assembly_gap    12532..12705
                     /estimated_length=174
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12706..12925)
                     /locus_tag="PK264_03970"
     CDS             complement(<12706..12925)
                     /locus_tag="PK264_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75085.1"
                     /translation="MTAANPDRVVLITGAASGIGAATARRIARRGLALAIHTRAESTE
                     SRVRLDETAAELRDQGAMVRTLTGDLAEP"
     assembly_gap    12968..13128
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13129..13992)
                     /locus_tag="PK264_03975"
     CDS             complement(<13129..13992)
                     /locus_tag="PK264_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD75086.1"
                     /translation="MTVSQSGPIETDVVIVGAGPIGLFAVFELGLVDVKAHLIDILGK
                     VGGQCAELYPEKPIYDIPALPIVSGQELTDRLMEQIKPFGATFHLGERVDQLEKLADG
                     RFSVRTDAGKDFICKVIVIAAGGGSFTPKRPPLDGIEAYEGKSVFYSVRRMEDFRGRD
                     VLIVGGGDSALDWTLNLQPIAKSLTLLHRRDDFRGAPHSVEKMRALVAEGKIALAIGQ
                     VTELHGADGMLDAVTIKTKDGDSRLACNRMMPFFGLTMKLGPIADWGLNLDENLIKVD
                     TEKFETSVPGLF"
     gene            14247..>14596
                     /locus_tag="PK264_03980"
     CDS             14247..>14596
                     /locus_tag="PK264_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75087.1"
                     /translation="MRLASVLNGLGGLVAMAGVLVAGVGVSTAHPPTILTGPAERAIS
                     EEVLAFRKSMAEVIARKDVSALRKMYFDGFVHTHTSGKQDGKDARIVSGLAGDPVIET
                     AAATEMVVRAPNDWT"
     assembly_gap    14597..14687
                     /estimated_length=91
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14846..15277
                     /locus_tag="PK264_03985"
     CDS             14846..15277
                     /locus_tag="PK264_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75088.1"
                     /translation="MPSLLISLLSALLVVGGLKLPSGGTGNGIVHEAKLGFSFMNAAQ
                     EQELWKRADGYALAEAFLKACSAPSYVERRMRVAADPCIEKAALDRVAVYFRKKVAEF
                     GRTKNFLCDTPHAKELVRSVRGRIDRDVEEVRTMCRNCFFC"
     gene            complement(15316..16014)
                     /locus_tag="PK264_03990"
     CDS             complement(15316..16014)
                     /locus_tag="PK264_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75089.1"
                     /translation="MPLAGSGFLAIWNDTADGGDAEWRQWHTVEHMPERVGVAGFLAG
                     RRYAARGPAAERYFTLYQGESIATFSSAPYLARLDNPTSWTVKTAPLFEKFLRGACRT
                     LASAGNGMGGAIATIRLAVSNPGRLERASGDICRRIVAGDIARDLIAAHIGRCDPNIT
                     GVPTRERAARGNRSEPVFDAVLLVEGIGGAAVEAGLPGLEALLQHEIHGAEPSVVGIY
                     ELEICLEKQHLPPT"
     gene            complement(15974..16630)
                     /locus_tag="PK264_03995"
     CDS             complement(15974..16630)
                     /locus_tag="PK264_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRD75090.1"
                     /translation="MAKVPVAGRVKTRLAKGVGVVRATAFFRHTARAVLGRIGSSPHW
                     QTIIAVSPDHARLSRAFPAGIARTPQGGGDLGARLARAIDRQPPGPVVVVGLDIPEIT
                     AAPIRKAFSLLGSNDAVFGAADDGGYWLVGLRRRPCSPRIFGRVRWSSEHALADTLAN
                     LAGKRVAMTGTLGDVDEAADLTKLEVEPGRRILPVAARHDCDWIRDASGRLGLSRHLE
                     "
     gene            complement(16708..17673)
                     /locus_tag="PK264_04000"
     CDS             complement(16708..17673)
                     /locus_tag="PK264_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD75091.1"
                     /translation="MAEISRRDVVIGASGAFALFGLAKPVAFIGTANAQLATTGHFKH
                     KVGDIEVVQLHDGIWEKAHDAGFIKNASIDDTKAALKGGGLTDAHVPINFTVTAIKTS
                     AGVVLIDSGTGGQTGGPKAGKLMANMAAAGIDPKSVKKIVVSHFHPDHIFGLMAKDTN
                     AQIFPEAEIVVPAAEYKFWTDPALPAKLPEARRPLAERIQKTMPTWKNVKPAEGEAEV
                     APGIRMVATPGHTPGHTSYHVSSGGQQLIVLGDVSNLPALFVKNPGWHAVFDQDAATA
                     EASRRKIFDRAIADKVTVAGYHFGMPNVGTLAKDGNGYAFTALKA"
     gene            complement(17845..18534)
                     /locus_tag="PK264_04005"
     CDS             complement(17845..18534)
                     /locus_tag="PK264_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HRD75092.1"
                     /translation="MISVIIPTLDAQDGLAATLTALVPAVVEGLVREVIVVDGGSADR
                     SVEIADQAGCTIVRSERGRGQQLATGAAAARHPWLLFLHADTILEQGWERDGSAFIER
                     IDSGLREPAAAAFRFALDDLGIAPRILEAGVAARCALFRLPYGDQGLLIPKRLYDRIG
                     GFSKVPLMEDVELVGRLKRSQLIMLRARAITSAIRYRRDGYLRRTLRNLSCLTLYYLR
                     VPPGRIVRWYR"
     gene            complement(18627..19478)
                     /locus_tag="PK264_04010"
     CDS             complement(18627..19478)
                     /locus_tag="PK264_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75093.1"
                     /translation="MFKADLLANKRILVTGGGSGLGAAMGRRFLELGAELVICGRRAD
                     VLSATAEQLSSETGGSVTPLRCDLRDPEAVEAMMSEIWQAGPLDILVNNAAGNFIART
                     ETLSHRAVDSVLGIVLHGSAYCTIAAGRRWIEAGKRGNVVLSILTLSALRGAAFRVPS
                     AMAKAGVHAMTKSLAAEWGPKGIRTAAIVPGWFPTDGASQRLRPTDDARPVTADIPLG
                     RAGEHAELANLAAFLVSDGAGYINGECVAIDGGRQFIGDGGGNTATLLGWGDAEWAAM
                     RRQAKGE"
     gene            19642..19806
                     /locus_tag="PK264_04015"
     CDS             19642..19806
                     /locus_tag="PK264_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HRD75094.1"
                     /translation="MRSPCVNVCVIDPVSGLCQGCFRTLAEIATWASLPEAERERIMR
                     DLPRRSGRPV"
     gene            19821..20519
                     /locus_tag="PK264_04020"
     CDS             19821..20519
                     /locus_tag="PK264_04020"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRD75095.1"
                     /translation="MAGLILLMLIVCGGAFLLVRSDPQLLEALSHPDSMMIAYGVIFL
                     IVVGVPLIATYRGRFGTAIRDLLAWTGLAAILVAGYSFREDLTGLYYRMAGELMPPGT
                     LIEGGTTPTGDRSVRIRRRPDSAKVGVNGTAVGMIIDTGASTVVLKQSDAVSIGIDPK
                     SLVYSVPVQTANGQTFAARVRLTSVSIGSIRVEQVEALVARPGALRESLLGMSFLSRL
                     KSYEFSGDYLTLRI"
     gene            20654..21130
                     /locus_tag="PK264_04025"
     CDS             20654..21130
                     /locus_tag="PK264_04025"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRD75096.1"
                     /translation="MDAIRAYIGPGEPREPPVATLAGVVAPSSADADKADRQVRIRAS
                     SSGHFETKVRLNGRHIDVLVDTGATLVALTYEDAVAAGISPRDSDFKFKVSTANGEAY
                     VAQVMIDEISIDDIVVRNVRGVVAERGKLGVTLLGMSFLSRLGKTEMTRDMLVLQQ"
     gene            complement(21138..22721)
                     /locus_tag="PK264_04030"
     CDS             complement(21138..22721)
                     /locus_tag="PK264_04030"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HRD75097.1"
                     /translation="MTETFPLNASPRVHYQTGEVDLVELAIRRGEGRFASSGALVVET
                     GLHTGRSAQDKFTVRDTTTENQIWWDNNKALSETQFAMLHADFLAEARKRDLFVQDLH
                     GGADPKHRIRVRVFTEYAWHALFIRHLLRRPAGADLSGFRPDLTIVNLPSFRADPKRH
                     GVRSETIIACNFAKRIVLIGGSSYAGETKKSVFSFLNYILPELGVMPMHCSANVGANG
                     DAAVFFGLSGTGKTTLSADPKRTLLGDDEHGWSESGIFNFEGGCYAKTIRLSQEAEPQ
                     IWAASNRFGTVLENVILRPGTGEPDFNDGRLTENTRSAYPLDFIANASTTGRSGHPKN
                     IVMLAADAFGVLPPIAKLSPSQAMYHFLSGYTAKVAGTEKGMGNEPQATFSTCFGAPF
                     MPRHPSVYGNLLRQLIGKHNVDCWLVNTGWTGGKFGTGQRMPIKATRALLDAALSGSL
                     KSQPMRTDPLFGFQVPTALAGVDAKILTPRDTWADKAAYDTQARGLVDMFNKNFSKFE
                     AHVDADVKAAAPTLRQAAE"
     gene            23063..23863
                     /locus_tag="PK264_04035"
     CDS             23063..23863
                     /locus_tag="PK264_04035"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRD75098.1"
                     /translation="MSADDEDHRAAGRAPRLFVEADLAANALIRLNEAQSGYLTQVLR
                     LGAGDAVLVFNGREGEWRASIAQTGKRRYELCLIDRSRLQICGSDIHYLFAPLKRARL
                     DYMVQKAVEMGVSELRPVMTRRTVAERVNLHRMRANAIEAAEQCGVLHVPAIREPTEL
                     GRVLDHWDASRPLIFCDEAARIADPLKALGRLRSKSLGSPTAVALLIGPEGGFSPEER
                     QRLLSLPFVAAISLGPRIMRADTAAVAALALVNAVLGDWSERDEVKGL"
     gene            23940..25322
                     /locus_tag="PK264_04040"
     CDS             23940..25322
                     /locus_tag="PK264_04040"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HRD75099.1"
                     /translation="MSTRQEGAPSPLITSKQDLIDWIAVGAKPRNQFRIGTEHEKVVF
                     YTSDHAPVPYAGPRGIRAIMERLIQRYGWLAITEGDNIIALRRPDGEAAATVSLEPGG
                     QFELSGAPLATMHEVSDETEEHLDQVRAVGEELGIGFLGVGFSPHWRLEEVPRMPKRR
                     YGVMTRYMPQVGRHGLDMMYRTATIQVNLDFADEADMVEKLRVSLALQPVVTAMFASS
                     PFTEGKPNGYKSFRSEVWRDTDRNRTGMLPFVFEPGMGYERYVDYALGVPMYFVYRDG
                     EYIDVAGASFKDFMAGKLAQLPGERPTMDDWSDHVTTLFPEVRLKRFLEMRGADGGRW
                     RRICALPAIWVGLLYDETAQKAAWDLVKSWSADERQGLRDGVPRLALETPFRKGSVHD
                     LAREVLAISRDGLKRRKRLDKKGQDERIYLAVLEDIVASGRTTADELLARYHGEWNKS
                     VLPIFKDYAF"
     gene            complement(25303..25878)
                     /gene="rsmD"
                     /locus_tag="PK264_04045"
     CDS             complement(25303..25878)
                     /gene="rsmD"
                     /locus_tag="PK264_04045"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRD75100.1"
                     /translation="MRIVGGRFRGRPIATPMPGGQAIRPTSDRVRQALFDILMHGIAD
                     FDLDGTRVLDLFAGTGALAFEALSRGAASAVLVDESAAARALMRETIVAIGLAGRARI
                     LKRDATDLGPAEAIPPANLVFADPPYGKSLGERAIASAIAGGWIAEGALIVLEEAEGS
                     TIGEIAGTAVIDERAWGGTLVRFMQLRTRSP"
     gene            complement(25875..>26627)
                     /locus_tag="PK264_04050"
     CDS             complement(25875..>26627)
                     /locus_tag="PK264_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRD75101.1"
                     /translation="RVAVNGETLASPARDVGPRDRITVDGNPLPVAEPARLWRYHKPR
                     GLVTSHNDPEGRPTVFSSLPPTLPRVISVGRLDYGSEGLLLLTNDGELARHLELPATG
                     WMRKYRVRAHGRISQADLDKLKDGIEVEGVQYGPVEATFDKEQGSNVWLTLGLREGKN
                     REVRRILSALGLEVNRLIRISFGPFQLLELAPGEAEIVRRRVLADQLGPELAEKFGLK
                     DDPEEVAVKPGKPQRAGEKRPRPRRPRDERPQ"
BASE COUNT         4258 a         8437 c         9061 g         4413 t
ORIGIN      
        1 ggtgtcgtcg accgaggggc cggcgcgcca gcggaaactg ctcccttgcg cggcgctctc
       61 gaaggccttg cgcgccttcg ccgagaattc gccgtcatcc cagtctgccg gcgggaaggt
      121 gacagcgacg atcgggtcgg gcgccacgat ctcgacgtgc atcgggcgga gtgcaacgcc
      181 gctcacggcg cccttcatgc gctcggcgaa aagccgcgcg gtcgccatga tcgaggtcat
      241 ggcatnnnnn nnnnngtcga cgggtacgag gatggacttc atcggccact cctccttgtt
      301 ttgctgttgc ggcacttgac ggatgcgcat cgttgcttga acggcatagg agatgcgcga
      361 ccggagcata gcatgatttg cccgcaggca acccccgagc ttcgccgatc gttgaactcg
      421 atcaggggat cgcggacggg gccggctcag tagcggaaca gcacgttgag cgtgccgtag
      481 aggccgtcgt cgcgccggag gttgtcgccg cgcgtctcgg cggcccctgc agaggccgag
      541 acctctccgc tcgcccattc gtagcgcacg aaggcgccgg cgcgcagcga ctgcagcgcg
      601 aggtcgccat tggcctgacc cgcgaggccg atcgagagcg cggggagtgc gcgccagccg
      661 gtcctcaagc gcgcgtcggc cgtgtcgcgc cccgtggtcc aggcgaggtc gagcgaagcg
      721 aatccggcgc ttccgatgtt gagccagctc tcgaggacga gcttggcgcc cgtgccatag
      781 ccggcgaggt cgaaatcggg gtccgccggc gtcacgtcgt agcgatcgag gtgggcgcca
      841 gcgaaagcct tcaccgtgag cgcacccatg ttcgcgtgat agccgagcag gatgtcggcg
      901 aacgaggaga cgccgtgatg gcggagcggc gtcgaggcgc tccccgccca atgatcgcgg
      961 acgtaggtgt agcgcccata gccactcacg gcccggatcc gccagccgtc cgcctgaatg
     1021 ccttcggaga gcgagaaggt ctggccgaca taggcggacc aggcatgccc catgacgtcg
     1081 gtgccggccc agatctcgcg ccagggcagc atcttgaatg tcgaaggatc ggcggactga
     1141 gctgaggcct cgcgagccac gccaagcagg ggcgcgaaga cggcacccgc cagcaacaca
     1201 ctccgcacaa cccgccgcat tccccgcccc tcgggattga cgccggccac aaatagtcaa
     1261 agcgcagaca actggggtat ctgcgcagtc cggggccaga aagtcaaatc acggattgat
     1321 cgggcgtgac cacgctcaga acttgaacgt gatgccggcg cgcagggtgt ggaagtcggg
     1381 atcgtagttg gtcgcggtgc cgccgtggat gatcctctca gcaccgaggt cggtgtaaat
     1441 gtactcgagc cggagcccaa cgtgatcgga gatggcgagg tcggcgccgg cacccagctg
     1501 atagccgaag aaggtctcgc cccgcgaggt ggtgccggcg agctgcaggt cgagatcggc
     1561 ccaggcgaga ccagccgtcg cgtagatcat cagggccggg ccgagcacga cgccggcgcg
     1621 gccgcggaac gatccgagcg cgttcacctc gctcgtcacg gtgccgaacg gggtcgcggc
     1681 actgccgccg agcccgcccg tgccgatgtc ggcttcgagg ccgagcacga tgccgcgcgt
     1741 cacttgccag ttgtagccca tgaagacggt gcccaatccg ccttcggtat cgaaggagaa
     1801 attgccgagg ccgccgtccg cggccgtcgt gccgaagccg agccccagcg tgccgccgag
     1861 atagaagccg tgccagcgcg ggtggaagat ctcgggccgc gtcaggtcgt agtagggctc
     1921 ggaggcgcgc acgggacgcg gtgcgccgag gtcggcggcc gatgccggag cagcagcgag
     1981 agcaaccgcg aggaccgcgg cgaacgagac gacgatacgc atcggcgatg gtcaccctgc
     2041 ttggcgtgct gcacggagca cgttcgagcc ccccagctcg atggctccgt gcgtagccat
     2101 ggaatagggc gacagctggc caggctcggg gcgatgtctg gacgagcaac ggccaaaaga
     2161 gggccgggcc cgcggcccgg tcgcgaagcg aaccttacgc tgcgtgcccg gcgcggcagt
     2221 cgcgcctcgt ccggatgccg ctgctgttgc cggtcgaaat gcgccgtccc gtcttgacag
     2281 ctcgcgcgcc cggccttgtc taggaggcga cagccatctg gagcgcacat gagcaccctt
     2341 cccaacctca agatcaacgg cggccgcctc tgggacagcc tcatggagat ggcnnnnnnn
     2401 nnnnnnnnnn nnnnngaaag gcggggtcaa ccgcctgacc ttgaccgacc tcgaccgaca
     2461 gagccgcgac ctcttcatcc gctggtgcaa agacgccggc tgcaccttga aggtcgacca
     2521 gatcggctcg ctgttcgcgc ggcgcaaggg ctccgaagac agcctgccgc cggtgatggt
     2581 cggcagccac ctcgacacgc agccgacggg cggcaagttc gacggcgcgc tcggcgtgat
     2641 ggccgcgctc gaggtcgtcc ggaccttgaa cgacctcgac gtgaggacga agcacccgat
     2701 cgagatctgc atgtggacca acgaggaggg ctcgcgcttt gcgcctgcca tgacggcctc
     2761 gggtgtgttc gcgggcgtgt tgaagctcga ggatgcgctg aagatcaagg acgtggacgg
     2821 gaaggaaatc ggcgctgagc ttgcccgcat cggctatgcc ggcaaggagc cggtcggccg
     2881 gcgcccggtg cacgcgctct tcgagctgca catcgagcag ggcccgatcc tcgaggagga
     2941 gaagatcgac atcggcatcg tcacggcggc gaacgggcag aagtggtacg agatcacgct
     3001 cgacggtgtc gagagccacg ccgggccgac gcccatgaac cggcgcaagg acgccctcgt
     3061 cggagcggcc cgcatcatcg acctcgtcaa caagatcggc catgcccatg atcccgcggc
     3121 gtgcgcgacc tgcggcatga tccaggccta tccgaactcg cgcaacgtga tcccgggccg
     3181 cgtattcctg actgtcgact tccgccatcc ggatgcggcc aagctcgcct cgatggatcg
     3241 ggagctccgc cgcggtatcg aggagattgt cgccaaggcg ggcctcaaag tcgacatgaa
     3301 gatggtcgcc gattatgcgc cgcagccctt cgatcccgcc tgcgtcgatg ccgtccgcaa
     3361 ggccgcgaag cgcctcgggc tctcggcccg cgagatcacc tcgggcgcgg ggcacgacgc
     3421 cgtctatatg gcgcgcatct gcccgacggc catgatcttc acgccctgtg tcgacggcat
     3481 cagccacaac gaggccgagg acatgacgcc cgcctgggcg gaggccggct gcaacgtgct
     3541 cgcgcacgcc gtgctcgaga aggccgaaat cgtcggcgcg cggtgagtcg caatcagttc
     3601 ccactttcta cacggagcct cagatgtcga agcttcagcg ccgcggtccg ctggtcggca
     3661 tgcgcgtcct cgatctcagc cacgtcatgg ccggaccgac ctgcgggcgc atgctcgccg
     3721 acatgggcgc ggacgtcatc aaggtcgaga aggcagatgg cggcgacgat acgcgcaaga
     3781 tgctgccacc tgacatcaag ggagagtcgg ccgcctacat gatgatgaac cgcaacaagc
     3841 ggggcatcgt gctcgatctg aagaagccgg ctgcccgcga ggcgctgctc gctctcgtga
     3901 aggcggccga cgtgctgatc gagaattacc ggcacgacac gctcgacaag ctcggcctcg
     3961 gttatgaggc actcgccaag gtcaatcccg gcctcatcta ttgcgcgatc tcgggcttcg
     4021 gccgcagtgg ccccttctcg gagcagggcg gctacgatct catcgcgcag ggcatgtcgg
     4081 gcctgatggc gatcacgggc gaggcgcccg ggcggcagcc ggtaaaggtc ggctcgcccg
     4141 cctgcgacat caccgcgggg ctccttggcg cgatgggcgt ttgcgccgcc tacgcaagca
     4201 agctcaagac ggggaagggg cagatcgtcg acacctcgct tttcgaggcc ggcatcacac
     4261 tcacctattg gcagtcggcg attgcctttg cgaccggcgt atcgccggga ccaatgggct
     4321 cggcacaccc gttgaatgcg ccctaccagg ccttcgagac gaaggacggc tggatgaatg
     4381 tcggtgcatc aaaccagaag atgtacccca tgctctgcga gcttctcggc gcgcccgggc
     4441 tcgcccgcga tccgcgcttc gtaacggggc gcgaccgcat gaacaatctt gccgtgctga
     4501 tcgagacgct cgccccctac ttcaagaagc gcacgacggc ggactggctc gtcgatctcg
     4561 agcggctcgg cataccggca ggtccggtgt tgtcgatccg tgagatgcat gctcatcctc
     4621 agacgctggc gcgcgacatg gtgccggcgg tcgagcatcc gattgctggc gcggtgcaga
     4681 cgatcggcct accggtgaag ttctcgggca ctccgggcga ggtgctgcat gcagccccgc
     4741 tgttcggcga acatacgcgt gaggtactgg tcgaggcggg gcttgcggcg gccgagatcg
     4801 atcgcctcat caaggagggc gccgcggtcg agacgcggcg gacggccaag gcctgatccg
     4861 gaccgcgggc gaggccacga tatcccgatg ctgccaacac tgtttgcctt tgcagtcgcg
     4921 ctcgccgttg tgcggctcct gttctccgag cttgcgcgaa acatcgcact cgaccagccg
     4981 aacgagcgct cgctgcatca gcggccgacg ccgcggaccg gcgggctcgg catcgtcgcg
     5041 ggcgtggcgg ccggctggct cgccgctgga ggagcacctt cagagatcgc gctgtcggcc
     5101 atgattcttg tgctcatctc gctcgccgac gatgtgctgg gcctcggctc ggggctgcgc
     5161 ttcggcgcgc agatcatcgc cgcggggctc ttcatcgcgg ggagtggtct ctccggcggg
     5221 ctcatggcgc tcgcagtcct cgtcggcatc gtctggtgcg ccaacctcta caacttcatg
     5281 gacggttcgg acggcatggc aggcggcatg gcggtgatcg gctttggcgc ctatgcgctg
     5341 atcgcggtgg cggcgggctc gcgatcacgg ccggggtgat cgcggcggcg gcggcgggct
     5401 tcctcgtctg gaatttccat cccgcgcgga tcttcatggg tgatgcgggg tccgtgccgc
     5461 tcggatttct cgcggccgcc atcgggcttc tcggctggca gcgcggcatc tggccgacct
     5521 gggtgccctg cctcgtcttc tcgccctttg cgatcgatgc gacggtgacg ctgctgcggc
     5581 ggatggcgcg cggcgaacgg ttctgggaag cgcatcgcac gcactattac cagcgcacga
     5641 tccagctcgg cctgggacat cggctggcgg cgcttgtggc ctacgccatc atgctcgccg
     5701 cagccatcac cgcgttcggg ttccgcaatg cttcgccgat catagtgacg gtcgtgctgg
     5761 cggcctgggc cgctgcctac gcattcctcg cgcgacggat cgatggcgcc tgggcgcggc
     5821 gaccgtcgcg cgcgggcccg agcggggcat gatctgacat caaccccgca cgcctctgag
     5881 cttggcgaac gtcgcaagag cccggtcgcg cgcgtcgcga tgctcgatga tcggtatcgg
     5941 ataggtctcg ccaagcttca cgttggcccg ggccaggatt tcgtcggtcg cgagccacgg
     6001 tgcatggatg tgggcgtcgg atagctcggc gagctccggc acccacttgc gcacgtagtc
     6061 gccgttggga tcgaaagtct gtccttgctt cacgggattg aaaatccgga aatagggtgc
     6121 cgcgtcggcg ccgcagcctg ccacccactg ccagcttgca gcgttattgg cgagatcggc
     6181 atcgacgagt gtatcccaga accaggcttc gccgtccttc cacgagacga gcagatcctt
     6241 caccaggaac gaggcgacga ccatgcgcac gcggttgtgc atgtagcccg tcgcccacag
     6301 ctcgcgcatg ccggcatcga cgatcgggta gccggttcgg cccttgcgcc aggccgcgag
     6361 cgcctgcggg tcggacgtcc actcgaactc ggcgaactcg ggccggaagg gctgctcggg
     6421 cagcgtcggg aaggtgtgca gcagattgac ggagaactcg cgccaggcga gctcgcgctg
     6481 gaagctcgag gcgccgttcg ccgcactcgg ctcgacggcc gcgcgagcgg cgaccgcatg
     6541 ccagcactgg cagggcgaca gctcgccgaa atggatgtgc ggcgacagtc gcgacgtgcc
     6601 gggaagatcg ggccgatcgc gatcttcggc ataaccggca agtgccatat ccagaaagcg
     6661 ctcgagttgc ttcagcgcgc cggcttcgcc tggatgccac gcggccttga gacccgttgc
     6721 ccaatctggc ttcttcggca ggagatgcca ggaggtgagg cgctcgctgg agggccagcg
     6781 cgacggcccc ttgatcgccg ccgggggcat gatcggcagc cgcggcggat cgagggagag
     6841 catagcccgc cagaacggcg tgaagacctt gaagggctcg ccgtgatgcg tcttcagcac
     6901 ctcgggctcg taaagcagcc cgccgccgta gcgtttcagc gcgacgccat cgcccgacag
     6961 gcgctcgttg agcgagcgct cgagcgtgcc ggcccaaggc tcatagcagc gtgtgcagtg
     7021 gatggcccgc gcgccggtct cgcggatgag atcgctcatc acgtcgagcg tgtcgccgcg
     7081 gcgcaggatg agcttcgagc cgagagcctt cagcgacgcc tgcagctgct caaggctgtg
     7141 gtgcaaccac cagcgactgg caccgcccat acgccagggc cccggcgtct cgtcgtccag
     7201 gatgtagacc ggaacgatcg gcacgtcggc ctgcacggct gctgtcagcg cccgatggtc
     7261 tcggatcctg aggtcctgtc gataccacag tatgatgggt gtagaagtca cggttgtacg
     7321 ttaagcctcg tggatatggc tcgacactct cggatcgaag tgatgccgac gggcgcgaat
     7381 ttcaattgcg tatcaactgc agcggcgttt ggtctcgtct cggtcgtttg aaagagttgg
     7441 ctcaattgaa gagagattcg aaccaggatt ttggcttgcc tgatttcgag ggagatttgg
     7501 gcggggcgcg gtcgggccgg gcataggtgg gattgtccgc ccagggtgcc catgcgggcg
     7561 gacccttggc cttcgcggtg gatggcgggg catcgacagc cgacagcgcc cgcttggggc
     7621 cgccaggacc ggcaacaggc ggcggggcaa gagcggagct tgcgcttcgg agcgcgacct
     7681 cctccgtctg tccgtcgagg ccggtaatgg tgagccgcgt cggtgtccaa gcaagcgttg
     7741 ctgatctcgc accggacttc aggagatcaa gagccagtat ggcgcggccc tgcaagtcga
     7801 tgacggcaaa acccacatgt tcctcgcggc tgccgccgcg ctcgactccc cccgcctcga
     7861 acgtgagcac accatagccg ggcggcggtg tcgtgacatc ccaatggagg agcttcgggc
     7921 gaccggaacc gacgacctcg agcggtacct gcaatcgggc cattcggctg ctcatcgcga
     7981 acagccgccg gccgacgacg tgcacacgcc actcgtcgat gccgagatcg gcaaccgcag
     8041 tctcctcggc gatcggcggt acgccaaggc gctcgagcgc cagcaccgcg tcgcgcaggt
     8101 cgggccgcag tgccacggcc ttgcgcagat cggcgatagc ctcttccttc tggccgaggc
     8161 tttcgcgaat ctcgccgcgc gcccagagca cctccgcccg ttccgggtcg agcttcaagg
     8221 cacgctcgat gtccttcaag ccgagctcgg cctgcccgga ctggcgatag gcccaggcgc
     8281 ggtaggcgaa ggtgaccggc gagcgcggat cgagctcgat gccgcgtgcg aagtcgttga
     8341 gggcctcgtc gtaggcttcg gccttggcgt ggccgaagcc gcgcgcggtg atggcggccg
     8401 ccgacttcgg ggctagttcg gtcgcgcggg tgaagtcctt caaggcgccg acgttgttgc
     8461 gcgcggcgag gtaggcgtag ccgcgcttca tgtagttctc gacgttttgc ggctcgaagg
     8521 cagtcgctcg actgagatcc tcgatggcct cctcgaagcg ttcgaggcgc atctttgctt
     8581 cggcccgcgc gcgataggcc gccgagaagc gcgcatcgat cgcgacggcc cggccgagat
     8641 cgcggactgc ggcgaatggc cggtcgagct tcaagtgcac ttgtgcgcgg ccgaggtagg
     8701 ggagggggct cgtcggcatc agctcgatgg cgcggccgta gtcgcgcagc gcctcgtccg
     8761 gggcatcgag cttcaggaag acgccggccc ggttgttgtg ggccgcagca taagccggtg
     8821 agagcacgat tgcccgatca aaatccttca cggcctcctt gagcaggcca agtgaggcga
     8881 gcacggtgcc ccggttattg taaagtgctg catattccgg atagagctgc gtcgcccggt
     8941 tgaaatcgtc gagcgcggcg cgggtctggc cgagggccgc aaaggcgacc gctcggtcga
     9001 ccaaaattgc tgcccggcgg tcgttgggaa gtgtcggatc cttcagcgct tcggaataaa
     9061 gatcggcggc tgactgcatc ttgccttcct gcagagcgcg cgcggcctcg gccatctttg
     9121 ccgcagcggc atccccggcc gcctcggcgg tccagcagag tgcgagcgcc atcagtgtcg
     9181 cgaccagggc tctcaaggcc atcatcaaca cctcgtttcg aggcagcaca actccacgct
     9241 tttccgatcg actttcaagc gacactcgcc ctctacagga tcagcgggca atgtggcaat
     9301 cttttaccgt caggtcgatg atcgaaaaac tcgaatgcgg gcaaagcact aatcagccct
     9361 tggccggtcc gtcagccggc ttgcggagct gacgggccag cttgtcgagt atcccgttta
     9421 ccactttcgg gccctcgtcg tcgagaaacg cgcgcgcgac ctcgaggtac tcggtgatga
     9481 cgaccttcaa cggcgtatcg gtacgctcga tcagttccca tacgccggcc ctcagcgtcg
     9541 ccctcagaat ggcatctatc cgcgtgaggc gccagccatc cgccagctga cggtctatga
     9601 tcggatcgat ctcgcgctgg cgcctgacga cgccgcggag cgtatcggcg aagagctggc
     9661 ggtccgcgtg cgcgagcgtt tggtcctcgg cgtcgggtgt gaggcggtag ctcaggaact
     9721 cgccgatcac gtcgttgagg tcggtgccgg cgacgtccat ctgatagagc gcctgcacgg
     9781 cggcgagccg cgctgcccgc cgcgccgtga gcccgccctc gctcgcgccc gtcattgggg
     9841 ccgtccgtgg agatcgtggg caatctcgat cagtctcagg caggcgcggg ccgcgtcgcc
     9901 acccttcccc gcaataccgc cgcgcgcgcg ggcaagtgcc tgctcgcgcg tgtcgacggt
     9961 gaggatcgcg ttgccgatcg ggatcgtgaa tgcgcgggcg acctcgtaga ggaagtggtt
    10021 cgcgttgttg cacacgatgt cgtagtggga cgtctccccg cggatgacgc agccaagcgc
    10081 gacgatgccg tcgaagcggg ccgtgcgcgc gctcgtacct gtgcgaccac aggcggcgac
    10141 gatttgagcg agctgtggca gctcgagcgc gccggggacg gagaagcgct cggcatgcga
    10201 tccgtccgcc tcgatcacgg cgagggcgcc ggcggccatt tcgtcggcga tgtcctcgta
    10261 gaaccgggcc tcgatgatcg caatccgtag accgccgatt cggcggctcg gtccgcttgc
    10321 cgatgtcgac gttcctgcca tggggttatc cgagctttct ggtgccgacg atccgcaggc
    10381 cgtggccttc gacgccgaca tagtgcgtgt ccggtgagtt cgtgagcagc gtcatctcgc
    10441 tgaccccgag atcgcgcagg atctgcgagc cgatgccgag ttcgacgagc gtgcgctctt
    10501 gcgtcttggc gcggccgccg ctggtggccg cggccttgat ccagctcgag accgatttgg
    10561 gcctcagatc gcgtagaagg acgagcacgc cgcggccctc agcctggatg gcggccagcg
    10621 ccttctcaat caggttgccg ctcccgctcg tcgcgccgat gccgagcacg tcggccggca
    10681 tgtttatagc gtgcacgcgg acgagcaccg gtcccggcgc cttgagatcg cccttcacga
    10741 gggcgagatg ctcggcgggc tcgacggccg tctcgtagac atgcaactcg agggagcccg
    10801 cgaacgcgct ctcgactgtc gtccgcgtcg tgcacttgac gattcgatca ttcttcaggc
    10861 ggtaggcgat caggtcctcg atggtgccga tcttcaggcc gtggcgctgg gcgaaggcga
    10921 cgaggtcggg catccgcgcc atggtgccgt cgtcgttcat gatctcgcag atgacgccgg
    10981 ccggatacag ccccgcgagg cgcgcgaggt cgacggaggc ttcagtgtgg ccggcgcgga
    11041 caagcgtacc gccttcgcgc gcgacgaggg ggaacacgtg ccccggcgag gcgatcgcgc
    11101 gatgatcctt ggtcgggtcg atggccgtcg agatggtgcg ggcgcgatcg gccgcggaga
    11161 tcccggtgga gataccttcg cgcgcctcga tcgatacggt gaatgccgtg cggttgcgtg
    11221 cctcgttgtt gcgcgccatg gcctcgaggc ggagttcgtt ggcgcgctcc tgcgggagcg
    11281 acaggcagat gaggccgcgg ccgtgcttgg ccatgaagtt gatcgcgtcc ggcgtcgcca
    11341 tctgtgccgg cacgatgaga tcgccctcgt tctcgcgatc ctcggcatcg acgagaatga
    11401 tcatgcggcc gttgcggaag tcctcgagga tgtcctcgat cggcgagatg aagcccttgc
    11461 cttccgcgta gactgcggtg cctaggcccc cgatcggttt tgacgacttg cccgggctca
    11521 ccgcgaggcc tccatgaaac gcgcaacgta acgcgcgaag atatcgacct ccagattgac
    11581 gaggtcgccc ggctttttgg cgccccatga cgtgaccgtc aaggtgtgcg ggatcaggtt
    11641 gatgccgaac cggtggccct cgacctcgtt caccgtcagc gacgtgccgt cgagcgcaac
    11701 ggagcctttg ggggcgatgt agcgcgcgag ctcggccggc acctcgagcg tgaagcggcg
    11761 cgacataccg tcgtcgtgca tggcggcaat gcgcgcgacg gcgtccacgt ggccgcttac
    11821 gacatggccg ccgaactcgt cgcctgcgcg cagcgcccgc tcgaggttga cgtggcggcc
    11881 ggccgcccac gaaccgagcg tcgtcttcga cagcgtctcg tttgagacgt cgaccgcgaa
    11941 gatgctgccg gcgccgaccg gggtgagcct ggtgacggtc aggcagcagc cgtcgcaggc
    12001 gatcgaggcg ccaatcgcga tcccatcggc cggatagcgc gagcggatgg cgaagcgtcc
    12061 gcccgtccgc tcctcgacca agccgatatc cggtgaacat gcctgcctcg cgcaccgatg
    12121 gttgcgagac tacaccgcca ctcaggcgag cgacagcccg ccgtcgacgg cgatcgactg
    12181 gccggtgatg tggcgggctt catccgagag gaggaaggcg atcagcgccg cgacgtcggc
    12241 gggatcggcg atgcggccga gcggcgtcag cctggcggcg gcgtcccagg ctgcggtgcc
    12301 gaggacgccg gcaccatgat ccttgcgcgt gtagccgggc acgaccgagt tcaccgtgac
    12361 ctgatgcggc gcgagctcga ttgcaagcgc ccgcgcaagc gcctcgccac cggccttggc
    12421 ggcggcagat gccgggaaat tggcgcccgc gacgaacctg tgcgcgacga atgaggagac
    12481 gaagacgatg cggcccgcgg gcgagccgac gagcgcatcg cgcgccgcat tnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccggc tcggcgaggt
    12721 cgcccgtgag cgtgcgcacc atcgcgccct ggtcgcgcag ctcggccgcc gtttcgtcga
    12781 gacggacgcg gctttccgtg ctctcggcac gcgtgtggat cgcgagcgcg aggccgcgtc
    12841 gcgcgatccg ccgcgcggtc gcagcgccga tgcccgaggc cgcgcccgtg atcagcacga
    12901 cgcgatctgg attggccgcg gtcatggcga ggctcctttc gagctacttg acgccgagct
    12961 tcttctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agaggccggg
    13141 cacgctcgtc tcgaattttt cggtgtccac cttgatgagg ttctcatcga gattgagccc
    13201 ccagtcagcg atggggccga gcttcatggt gaggccgaag aacggcatca tgcgattgca
    13261 ggcgaggcgg ctgtcgccgt ccttcgtctt gatcgtgacg gcgtcgagca tgccatcggc
    13321 gccgtgcagc tccgtcacct ggccgatggc gagggcgatc tttccctcgg cgacgagcgc
    13381 gcgcatcttc tcgaccgaat gcggggcacc gcggaaatcg tcgcggcggt ggagcagcgt
    13441 caggctcttg gcgatcggct gcaggttgag cgtccagtcg agcgcgctgt cgccgccgcc
    13501 gacgatcagc acgtcgcggc cacggaaatc ctccatgcgg cgcaccgagt agaagaccga
    13561 cttgccttcg taggcctcga tgccatcgag cggcggacgc ttcggcgtga acgaaccgcc
    13621 acccgccgca atcacgatca ctttgcagat gaaatccttc ccggcgtcgg tgcgcacgga
    13681 aaagcgcccg tccgcgagct tctccagctg atcgacacgc tcacccagat ggaacgtcgc
    13741 gccgaacggc ttgatctgct ccatcagccg gtcggtcagc tcctggccgg aaacgatcgg
    13801 aagggccgga atgtcgtaga tcggcttctc ggggtagagt tcggcgcatt ggccgccaac
    13861 cttgcccagg atgtcgatga gatgggcctt cacgtcgacg aggccgagct cgaagacggc
    13921 aaagaggccg atcgggccgg cgccgacgat gacgacatcg gtctcgatgg ggcctgattg
    13981 gctcacggtc attttgtcct gctcctgggc acggttggcc tagtgccgcg ccgtcgcacg
    14041 attggatcgc acagggcgga ggcacgaggc atgacgacgg tcaatgaggg gcttagcgcg
    14101 gtgagtggaa ccgggcaacc ggcgagggtt gcagccctaa tgggtgagca cacgagcgcc
    14161 gggtcaccag taagggcgca acttgtcgcg ccgcgatgca attggtagcc tcgatccgat
    14221 cgtgtgtcgt gtgctggagg tcggagatgc ggctcgcatc cgtgttgaat ggtctcggcg
    14281 ggctcgtcgc aatggctggt gtgttggtcg caggtgtcgg ggtgagcacg gcccatcctc
    14341 cgacgatcct gacgggtccg gcagagagag ccatcagcga ggaggtgctc gcgttccgca
    14401 agtcgatggc ggaggtgatc gcgcggaagg acgtatccgc actgcgtaag atgtacttcg
    14461 acggctttgt ccacacccac acgtccggaa agcaggacgg taaggatgct cgaatcgtat
    14521 cgggactcgc gggtgacccc gtcatcgaga cggcggcggc gaccgagatg gtcgtacggg
    14581 cgcccaacga ctggacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg cggcgagtca
    14701 ggcgacacgc agccatgaga tcaagccgtg aggcgcgaag cacgcacccg tcgatgcaac
    14761 tcgcagtggc acccagttcc ggctggacct cagggctcac ctggataaga tacgcgcctc
    14821 gagctcaact gatgcggagc cgttcgtgcc gagccttctg atttccctct tgtcggcgct
    14881 tctcgtcgtc ggcggtctca agctgccgag cggcggtaca ggaaatggga tcgtgcacga
    14941 ggcgaaactc ggtttcagct tcatgaacgc ggcgcaggag caggagctct ggaagcgggc
    15001 cgacggctat gcgctggccg aggcattcct gaaagcctgt agcgcgccct cctatgtcga
    15061 gcgccgcatg cgcgtggccg ccgatccctg catcgagaag gccgcactgg accgcgtcgc
    15121 cgtctacttc cgcaagaagg tcgccgagtt cgggcgcacg aaaaacttcc tctgcgatac
    15181 gccccacgcg aaggagctcg tgcgcagcgt gcgcggccgc atcgaccgcg atgtggagga
    15241 ggtgcgcacg atgtgccgga actgcttctt ctgctgagag ccctcccggc gcggagtgtc
    15301 gccgggtcga tcgagctatg tcggcggcag atgctgtttc tcgaggcaga tctcgagttc
    15361 gtagatgccg acgaccgacg gctcggcgcc gtgaatttcg tgctgcagca gcgcctcgag
    15421 ccctggtagt ccggcttcga cggccgcgcc gccgatgccc tcgaccagca gaacggcatc
    15481 gaagacaggc tcgctccgat tgccgcgcgc cgcccgttct cgcgtgggaa cccccgtgat
    15541 attgggatcg cagcggccga tgtgggcagc aataagatcg cgagcgatgt cgcccgcgac
    15601 gatgcgccgg cagatgtcgc cggaggctcg ctcgagccgg ccagggttcg agaccgccag
    15661 gcggatggtg gcaatggccc cgcccatgcc gttgccggcg gaggcgagcg tgcggcaggc
    15721 gccacgcagg aacttctcga agagaggggc agttttcact gtccacgatg tcgggttgtc
    15781 gaggcgggcg aggtagggcg cgctcgagaa cgtcgcgatg ctctcaccct gatagagcgt
    15841 gaagtagcgc tcggccgccg ggccgcgcgc tgcgtaccgg cgaccggcga gaaagcctgc
    15901 gacgccgacc cgctccggca tatgctcgac cgtgtgccac tgacgccatt cggcgtcgcc
    15961 gccgtcggcc gtgtcattcc agatggcgag aaagcccgag ccggccagag gcatcgcgta
    16021 tccaatcaca gtcatggcgc gccgcgaccg gcaggatccg ccggcccggt tcgacctcga
    16081 gtttggtgag atcagctgct tcgtcaacat cgccgagcgt gccggtcatt gcgacacgtt
    16141 tgcccgcgag attggcgagc gtgtcggcga gcgcatgctc gctcgaccag cgcacccgcc
    16201 caaagatgcg cggagagcac gggcgacgcc tgaggccgac gagccaatag ccgccatcgt
    16261 ccgcggcccc gaagacggcg tcgttgctcc cgagcaggga aaaggccttt cgaatgggcg
    16321 ccgcggtgat ctcaggaata tcgagcccga ccaccacgac cggtccaggc ggctgccgat
    16381 cgatcgctcg tgcgagccgc gccccgagat ctccgccgcc ctggggagta cgcgcgatcc
    16441 ccgccgggaa ggctcgcgac aatctggcgt ggtccggcga tacggcgatg atcgtctgcc
    16501 aatgcggcga gctgccgatg cggccgagca cggcgcgcgc cgtatggcga aagaaggctg
    16561 tggcgcgcac cacgccgaca cccttcgcga gtcgcgtctt gacgcgtccg gcgacgggca
    16621 ccttcgccat caccacgagc cgacagcgaa agggcgccgc gcttttgcgc gacgccctgt
    16681 tctctcgatc cggtcggttg gccggcatca ggccttcagc gccgtgaacg cgtagccgtt
    16741 tccgtccttg gccagcgtgc cgacattcgg catgccaaag tggtagccgg ctaccgtgac
    16801 cttgtcggcg atcgcacggt cgaaaatctt gcggcggctc gcctcggcgg tggcggcgtc
    16861 ctggtcgaag acggcatgcc agccggggtt cttcacgaac agcgccggca ggttcgacac
    16921 gtcgccgagc acgatgagct gctggccgcc cgaggagacg tgatagcttg tgtggccggg
    16981 cgtgtgaccg ggcgtcgcga ccatgcggat gccgggggcg acctcggcct cgccctcggc
    17041 cggcttcacg ttcttccagg tcggcatggt cttctggatg cgctcggcaa gcggacggcg
    17101 tgcctcggga agttttgccg gcaacgccgg atcggtccag aacttgtact ctgccgccgg
    17161 cacgacgatc tcggcctcgg ggaagatctg cgcgttggtg tctttcgcca taaggccgaa
    17221 gatgtggtcg gggtgaaagt gggagacgac gatcttcttc accgacttcg ggtcgattcc
    17281 ggcggctgcc atgttggcca tcaactttcc ggccttcggg ccgccggtct gaccccctgt
    17341 gccgctgtcg atgagaacga cgccggcgct cgtcttgatg gccgtcaccg tgaagttgat
    17401 cggcacatgc gcgtctgtaa ggccgccgcc cttaagggcg gccttggtgt cgtcgatcga
    17461 ggcgttcttg atgaagccgg cgtcgtgcgc cttttcccag atgccgtcgt ggagctggac
    17521 gacttcgatg tcgccgacct tgtgcttgaa gtggccggtc gttgcgagct gggcattggc
    17581 cgtgccgatg aaggcaaccg gcttcgcgag cccgaacagt gcgaaggcgc cggaggcgcc
    17641 gatgacgacg tcgcgccgtg agatttcagc catatgtgtt tcctccttga gttggcgccc
    17701 gcggaaccgg tcgatccgct caggcaccaa gggccgctgc tcatagcgcg gccgttgcgg
    17761 cagttatgcg acggaatggg cttgccggcg acaggctcac gggaagttga tagcgcgtgt
    17821 tctcgcccgg gttcgggctg gcgctcaccg gtaccagcgg acaatgcggc ccggagggac
    17881 gcggagatag tagagcgtga ggcacgacag gtttcgcagc gtccggcgaa ggtagccgtc
    17941 ccgacgatag cggatcgcgg aggtgatggc gcgcgcacgc agcatgatga gctgcgagcg
    18001 cttaaggcgc ccgaccagct caacatcctc catgagcggc accttcgaga agccgccgat
    18061 gcggtcatag agccgcttcg ggatgaggag gccctggtcg ccgtaaggga ggcggaacag
    18121 ggcgcagcgg gcggcgacgc cggcctcgag gatgcgcggc gcgatgccga gatcgtcgag
    18181 agcgaagcgg aacgcggcgg cagccggctc cctgagaccc gagtcgatgc gctcgatgaa
    18241 ggccgagccg tcgcgctccc agccctgctc gaggatggtg tcggcgtgca ggaagaggag
    18301 ccagggatgg cgggctgctg cggcgccggt ggcaagttgc tgtccacggc cacgctcgga
    18361 gcgcacgatc gtgcagccgg cttggtcggc gatctcgacg ctgcggtcgg ccgagccgcc
    18421 gtcgacgacg atcacctcgc gcacgagacc ttcgacgacg gccggcacga gcgcggtcaa
    18481 ggtcgcggcg agaccatctt gcgcgtcgag ggtcgggatg atgaccgaga tcatggcagc
    18541 ggtccgcgaa ggtgagccgg atgtgggcct tatagtgcgc tcgggcgcag aggcaaaaac
    18601 aatgatgatc accgtggcgt gttgagctac tcgcccttgg cctggcggcg catcgcagcc
    18661 cattcggcat cgccccagcc gagcaatgtc gcggtgttgc cgccaccgtc gccgatgaac
    18721 tggcggccgc catcgatcgc gacgcactcg ccgttgatgt agccggcgcc gtccgagacg
    18781 agaaatgccg cgagattggc gagctcggcg tgctcgcccg cgcgaccgag cgggatatcg
    18841 gcggtcacgg gacgcgcgtc atccgtcggg cggagccgct ggctcgcccc atcggtcggg
    18901 aaccagcccg gtacgatggc ggcggtgcgg atgcccttcg gcccccactc ggcggcaagg
    18961 ctcttcgtca tcgcgtggac gcccgccttg gccatcgccg aggggacacg gaaggccgcc
    19021 ccgcggagcg ccgaaagcgt gaggatgctc aagacgacgt tcccgcgctt cccggcctcg
    19081 atccagcggc gtccggcggc gatggtgcag taggccgagc catgcagcac gatgccgagg
    19141 accgaatcga ctgcgcgatg cgagagcgtc tcggtgcgcg caatgaagtt gccggcggcg
    19201 ttgttgacga ggatgtcgag cgggccggcc tgccagatct cgctcatcat ggcttcaacg
    19261 gcttcggggt cgcggaggtc acagcgtagc ggcgtcacgc tgcccccggt ctcgctggag
    19321 agctgctcgg cagtcgcaga gagcacgtcg gcgcgcctcc cgcagatcac gagctcggcg
    19381 ccgagctcga ggaagcggcg ccccatggcg gcgccgagcc cggagccgcc gcccgtgacc
    19441 aggatgcgct tgttggcgag aagatcggcc ttgaacattg tactaccctc gggctgcgct
    19501 cggacctcgc atcgacaacg gcccgtagac tgtcgatggg gccggcacgc ggccgccaca
    19561 atggcgatcc agtatcggta cacgcgaatc gcgtctgtgc tatccaacgc gaccgtgatg
    19621 ccgcttggac atgagcctcg catgcgctcg ccgtgtgtca acgtctgtgt catcgatccg
    19681 gtctctggcc tctgccaggg atgctttcgg acgctggccg agatcgcaac ctgggcgagc
    19741 ttgccggagg cggagcgcga gcgcatcatg cgtgatctgc cgcggcgcag cggacgaccc
    19801 gtttgagcgg ggagggtgat gtggcgggct tgatactctt gatgttgatc gtctgcggcg
    19861 gggcgttcct gctcgtgcgc tcggatccac agctgctcga ggctttgagc catccggaca
    19921 gcatgatgat cgcctacggt gtgatcttcc tgatcgtcgt cggcgtgccg ctgattgcga
    19981 cctatcgtgg ccgcttcggc acggcgatcc gtgatctgct ggcctggacg gggctcgccg
    20041 ccatcctggt cgcgggctac agcttccgcg aggatctgac cggcctctac tatcggatgg
    20101 ccggcgagct tatgcctccc ggcacgctta tcgagggtgg cacaacacca accggcgacc
    20161 gatccgtgag gatccggcgg cggccggata gcgccaaggt cggcgtcaac ggcaccgccg
    20221 tcggcatgat catcgataca ggtgcttcga cggttgtcct caagcagtcc gatgcggtgt
    20281 caattggcat cgatccgaag agcctagtct actcagtccc cgtccagacc gccaacgggc
    20341 agacgtttgc ggcgcgcgtc cggctcacca gcgtcagcat aggctcgatc cgggttgagc
    20401 aggtcgaggc gctcgttgca agaccgggcg cattgcgcga aagcctgctc ggtatgagtt
    20461 tcttaagccg tctcaagtct tatgagttct ctggggacta tttgaccctg cggatctgag
    20521 ccggctgccg gcgctggccc gcaggccatc gttggtcgat ccgatgtcgc tttcctccgg
    20581 tacacgcaac tcgttggccc tcgcgggctc ctggatcggt gcggcttcca tcttggtggt
    20641 tggcctcgca aacatggacg cgatcagggc ctacatcggt ccgggggagc cccgcgagcc
    20701 gccggtcgcc acactcgccg gcgtggtcgc gccatcttcg gcagatgccg acaaggccga
    20761 ccggcaggtc cgcattcgtg cttccagcag cggccacttc gagacgaaag tccgcctcaa
    20821 cgggcgccat atcgacgtgc ttgtcgacac cggggcgacg ctcgttgcgc taacgtacga
    20881 agacgcggtc gcggccggga tcagcccgcg cgacagcgat ttcaagttca aggtcagcac
    20941 ggccaacggc gaggcctacg tggcgcaagt catgatcgac gagatcagca tcgacgatat
    21001 cgtcgtgcgc aacgtccgcg gcgtcgtcgc cgagcggggc aagctcggcg tcacgctcct
    21061 cggcatgtcg tttctgtcgc gcctcggcaa gaccgagatg acgcgcgaca tgctcgttct
    21121 gcagcagtag gccgagctta ctccgccgcc tgcctgagcg tcggcgccgc cgccttcacg
    21181 tcggcgtcca cgtgcgcctc gaacttcgag aagttcttgt tgaacatgtc gacgagacca
    21241 cgggcctgcg tgtcgtaggc ggccttgtct gcccaggtat cgcgcggcgt caggatcttg
    21301 gcgtcgacgc cggcaagcgc tgtcggaacc tggaagccga acagcggatc ggtgcgcatc
    21361 ggctgcgact tgagcgagcc cgagagcgcc gcatcgagca gtgcgcgcgt cgccttgatc
    21421 ggcatgcgct gaccggtgcc gaatttgccg cccgtccagc ccgtattgac gagccagcaa
    21481 tcgacattgt gcttgccaat gagctggcgc agcaggttgc cgtagacgct cggatggcgc
    21541 ggcatgaagg gcgcgccgaa gcaggtcgag aaggttgcct gcggctcatt gcccatgccc
    21601 ttctcggtgc cggcgacctt tgccgtgtag cctgagagga aatggtacat cgcctggctc
    21661 ggcgagagct tggcgatcgg gggaagcacg ccgaaggcgt cggccgcgag catcacgatg
    21721 ttcttcgggt ggccggagcg gcccgtcgtg ctcgcattgg cgatgaagtc gagcgggtag
    21781 gccgagcgcg tgttctcggt gagccggccg tcgttgaagt cgggttcgcc cgtgccgggg
    21841 cgcaggatca cgttctcgag cacggtgccg aagcggttgg acgcggccca gatctgcggc
    21901 tcggcctcct gcgagaggcg gatcgtcttg gcgtagcagc cgccctcgaa attgaagatg
    21961 ccgctctccg accagccgtg ctcgtcatcg ccgaggaggg tgcgcttcgg gtcggcggag
    22021 agcgtcgtct tgccggtccc ggagaggccg aagaagacgg ccgcgtcgcc gttggcgccg
    22081 acattggccg agcagtgcat gggcatcacg ccgagctcgg gcaagatgta gttgaggaag
    22141 gagaacaccg actttttcgt ctcgccggcg taggaggagc cgccgatcag cacgatgcgc
    22201 ttcgcgaaat tgcaggcgat gatcgtctcg gagcgcacgc catggcgttt cgggtcggcg
    22261 cggaagctcg gcaggttgac gatcgtcaga tccgggcgga agccggagag atcggcaccg
    22321 gcaggccggc gcagcaggtg gcggatgaag agcgcgtgcc aggcatactc cgtgaagacg
    22381 cgcacgcgga tgcggtgctt cgggtcggcg ccgccgtgca ggtcctgcac gaagagatcg
    22441 cgttttcgcg cctcggccag gaagtcggcg tgcagcatcg caaactgcgt ctcgctcagc
    22501 gccttattgt tgtcccacca gatctggttc tcggtcgtgg tatcgcggac cgtgaacttg
    22561 tcctgcgccg agcggccggt gtgtagcccc gtctcgacga ccagggcgcc cgacgacgcg
    22621 aagcggcctt cgccgcgccg gatggcgagc tcgaccagat cgacctcgcc ggtctgataa
    22681 tgaacgcgtg gcgaagcatt gagggggaag gtttcggtca tcgcgttcaa gccctgcggg
    22741 tccatcgtga gtgatacgtt accatcgtgg cccggccgaa agtaccgcga ggcggtgact
    22801 ttcgacagga gcgcgtcgtc cgaatgcttg ccaatcggtt atggcgcctc gatgttggcg
    22861 cgacgaggcg gccggttcgc ttggcagatg cacgacaggc ccggtctcca cgaccaggcc
    22921 gttctggtct agaaagccga aagtagggcc tcaattcgtc caagagtcgc agcttgctga
    22981 aatattaccg tgttcacagt gcgatttgcg cttcgatggc tcatcacgcc aaccgtccgc
    23041 ggcgagggag atcgacgaag gtatgagtgc agacgatgag gatcatcggg cggccggacg
    23101 ggcgccacga ctgttcgtcg aggcggacct cgccgccaat gccttgataa ggctgaatga
    23161 agcccaatcg ggctatctga cccaggtact ccgcctcggc gccggcgatg ctgttctcgt
    23221 cttcaacggt cgcgaggggg agtggcgagc ctcgatcgcg cagaccggca agcggcgtta
    23281 cgagctttgc ctcatcgacc ggtcgcggct tcaaatctgc ggatccgata tccactatct
    23341 cttcgcgccg ctgaagcgtg cgcgcctcga ctacatggtc cagaaggccg tcgagatggg
    23401 cgtctcggaa ctgagaccgg tgatgacccg gcgcaccgtc gccgagcggg tcaatctcca
    23461 tcgcatgcgc gccaatgcca tcgaggcggc ggaacagtgc ggcgtgctgc acgtgccggc
    23521 gatccgcgag ccgaccgagc ttggccgcgt cctcgatcat tgggacgcgt cgcggccctt
    23581 gatcttctgt gacgaggccg cccgcatcgc cgatccgctc aaagcactcg gccgactgcg
    23641 ctcaaagagc cttggttcgc cgacggccgt cgcactcttg attggtcccg aaggcggctt
    23701 cagcccggag gagcggcagc gccttctgtc tcttcccttc gtggcggcca tctcgctcgg
    23761 cccccgcatc atgcgtgccg atacggcggc ggtcgcggca ctcgcactcg tgaatgcggt
    23821 gctcggcgac tggtcggagc gcgacgaggt caagggcttg taagccgacg gccggacgtc
    23881 cataactttt cgcgaaacct ttgtccgggc gaccgcgcgt gcgcccgagg agcattttca
    23941 tgtcgacacg ccaggagggc gcaccctctc cgctgatcac gtcgaagcag gatctcatcg
    24001 actggatcgc cgtcggcgcc aagccgcgga accagttccg cattggcacc gagcacgaga
    24061 aggtggtgtt ctacacctcc gatcacgcgc cggtcccgta cgctggccct cgcggcatcc
    24121 gtgcgatcat ggagcggctc atccagcgct atggctggct cgcgatcaca gagggtgaca
    24181 acatcatcgc gctgcgccgt cccgatggcg aggctgcggc gaccgtctcg ctggagcccg
    24241 gcggccagtt cgagctctcg ggagcgccgc ttgcgaccat gcacgaggtg tccgatgaga
    24301 ccgaggagca cctcgatcag gtgcgcgccg tcggcgagga gctcggcatc ggcttcctcg
    24361 gcgtcggctt ctcgccgcac tggcgcctcg aggaggtgcc gcgcatgccg aagcggcgct
    24421 atggcgtcat gacacgctac atgcctcagg tcggccggca cgggctcgac atgatgtacc
    24481 gcacggccac gatccaggtg aacctcgact tcgccgacga ggctgacatg gtcgagaagc
    24541 tgcgcgtctc gcttgcgctg cagcccgtcg tcacggcgat gttcgcctcc tcgcccttca
    24601 ccgaaggtaa gccgaacggc tacaaatctt tccgcagcga ggtctggcgc gacacggacc
    24661 gcaatcgcac gggcatgctg ccgttcgtgt tcgagccggg catgggctac gagcgctacg
    24721 tcgactatgc gctcggcgtg ccgatgtact tcgtctaccg cgacggagag tacatcgacg
    24781 tcgccggcgc ttcgttcaag gacttcatgg cgggcaagct cgcacagctt cccggcgagc
    24841 gtccgacaat ggacgactgg tcggaccacg tgacgacact cttccccgaa gttcgcctga
    24901 agcgcttcct cgaaatgcgc ggcgccgacg gcggacgctg gcgtcgcatc tgcgcgctcc
    24961 ccgcaatctg ggtcgggctc ctctacgacg agacggcaca gaaagccgcc tgggacctcg
    25021 tcaagtcgtg gagcgcggac gagcggcagg ggttgcggga cggtgtgccg cgcctcgccc
    25081 tcgaaacgcc gtttcgcaag ggcagcgttc acgacctcgc gcgcgaggtg ctggccatct
    25141 cgcgcgatgg cctgaagcgg cgcaagcgcc tcgacaagaa gggccaggac gagcgcatct
    25201 accttgccgt gctcgaggat atcgtcgcct ctggccgcac gacggctgac gagctcctcg
    25261 cgcgctatca cggcgagtgg aacaagagcg tgctgccgat cttcaaggac tacgcgttct
    25321 gagctgcatg aagcgtacga gcgtgccgcc ccaggctcgc tcgtcgatca ccgccgttcc
    25381 ggcgatctcg ccgatcgtcg agccctcggc ctcctcgagc acgatgagcg cgccctccgc
    25441 gatccagccg ccagcgatgg ccgaggcgat ggctcgctcg ccgaggctct tcccataggg
    25501 cggatcggcg aagacgaggt tcgccggcgg gatcgcctcg gcgggtccga ggtcggtcgc
    25561 gtcgcgcttc agaatgcggg cgcgccccgc gaggccgatt gcgacgatgg tctcgcgcat
    25621 cagtgcgcgt gcggcagcgc tctcgtcgac aagcaccgcg ctggcagctc cccgcgacag
    25681 cgcttcgaac gcgagcgcac ccgttcccgc gaagaggtcg aggacgcggg tgccgtcgag
    25741 atcgaagtcg gcgatgccat gcatgaggat gtcgaatagt gcctggcgga cacggtccga
    25801 ggtcggtcgg atcgcctgac ctcccggcat cggcgtcgcg atcggacggc cacggaacct
    25861 gccgccgacg atcctcattg cgggcgctcg tcgcgggggc ggcgcgggcg gggccgcttc
    25921 tcgcccgcgc gctgcggctt gcccggcttc accgcgacct cttccgggtc gtccttcaga
    25981 ccgaacttct ccgcgagctc gggaccgagc tgatcggcga gcacgcggcg ccgcacgatc
    26041 tcggcctcgc cgggcgcgag ttccagcaac tggaacggcc cgaacgagat gcggatgagg
    26101 cggttcacct cgaggccgag ggctgagagg atgcgacgta cctcgcggtt cttgccttcg
    26161 cggagtccga gcgtcagcca gacgttgctg ccctgctcct tgtcgaaagt ggcctcgaca
    26221 ggtccatact gcacaccctc gacctcgatg ccgtccttga gcttgtcgag gtccgcttgc
    26281 gagatgcggc catgcgcacg tacgcggtat ttccgcatcc aacccgtcgc cggcagctcc
    26341 aggtggcgcg cgagctcgcc atcgttggtg aggaggagca ggccttcgct accgtagtcg
    26401 aggcggccga cggatatgac gcgcggcaag gtcggaggga gcgaagagaa gacggtcggc
    26461 cggccctcgg gatcgttgtg gctcgtcacc aggccgcgcg gcttgtggta gcgccagagg
    26521 cgggccggct cggcgacggg gagaggattg ccgtcgacgg tgatgcggtc gcgcgggccg
    26581 acatcgcggg cgggcgaggc gagcgtctcg ccgttgacgg cgacgcg
//
LOCUS       DAORNH010000022        26063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1439_length_26063_cov_2.977622, whole genome shotgun sequence.
ACCESSION   DAORNH010000022 DAORNH010000000
VERSION     DAORNH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26063
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1439_length_26063_cov_2.977622"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1536)
                     /gene="ppsR"
                     /locus_tag="PK264_04055"
     CDS             complement(127..1536)
                     /gene="ppsR"
                     /locus_tag="PK264_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010109 - regulation of photosynthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PpsR"
                     /protein_id="HRD75102.1"
                     /translation="MRSLRKAISSLNAEAATRLVTVATDLAVMMDPDGTVCDVAICSD
                     SLSKESDVHSWIGRNWIDTVTIESRPKIAQLLSDASTIARQGAQWRQVNHPNPNGRGD
                     LPVRYATMRIGQGGRILALGRELRSMSLLQQKLLETQQAVEREHARVRSAETRFRLLF
                     QIAGDAVIIADAATLRISDANPAAQHLLEPAARRISGKTVVELFQSSSQGAVQRMLDG
                     LRTSGRADPLTSRLANARTEVVVTGSLFKQEATAHLLLRLVPADVTERTDAPSSGETL
                     RALVESMPDAFVVSGLDRRVLMANAAFLELAQMSSLEQIRGQPLDRWVGRTPAETNLL
                     IGSLREHGSVRSFRTVVQGELGSSDDVEVSAVTVESGEQPCFGFTIRSIGRQLAAEGT
                     LSQSLPRSASQLAGLVGQVPLKNLVREATDLIERMCIETALQIVDDNRASAAEMLGLS
                     RQSLYMKMRRYGLNDPSGD"
     gene            complement(1650..2546)
                     /locus_tag="PK264_04060"
     CDS             complement(1650..2546)
                     /locus_tag="PK264_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HRD75103.1"
                     /translation="MVTLTRWNGPSSGCREESRPGDGTRPDIRGSLQTRLSSPRDTIV
                     QEMLLRCAIETEIAPRLMLLTRQQAAQLGERASGRITIIDEDVATLCTALLDSDTARV
                     AELVDAFLVQGASVAQVLLELFAPAARRLGELWESDERTFAEVTMAMGELQRAMHHLD
                     EASGRSARQGTLKGRSILLVPAAGEQHTFPLSILDAFFTMAGWIVEAHFELKIGAVCA
                     RLRRHATDVVGMSISRESLLGQAASDIDRMRQASINPRLQVLVGGRALETDPNVVARL
                     GADGSASDARSALLAAERLVAG"
     gene            2871..3395
                     /gene="bchF"
                     /locus_tag="PK264_04065"
     CDS             2871..3395
                     /gene="bchF"
                     /locus_tag="PK264_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-vinyl bacteriochlorophyllide hydratase"
                     /protein_id="HRD75104.1"
                     /translation="MRDAPLSKPRSQALYSVDERRRRDASPWTIVQGVLAPVQFVIFL
                     VSAALVLRFLANGEGYVAATASIVLKTLALYAIMITGSIWEKAVFGRYLFAPAFFWED
                     VVSMLVLALHTAYLVVLVLGLMPATGQMILALLAYASYVINAGQFLLKLRRARIEQPQ
                     DALARVRLGGEALG"
     gene            3395..4687
                     /locus_tag="PK264_04070"
     CDS             3395..4687
                     /locus_tag="PK264_04070"
                     /EC_number="1.3.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) subunit N"
                     /protein_id="HRD75105.1"
                     /translation="MPSAALAPAAFAESSGSQRYVLRERGQREVFCGLTGIVWLHRKI
                     QDAFFLVVGSRTCAHLLQSAAGVMIFAEPRFATAIIDERDLAGLADANDELDRVVAEL
                     LARRTGIKLLFLVGSCPSEVIKLDLGKAAERLSRRHAPATRILHYSGSGIETTFTQGE
                     DACLQALVGAMPATAADSPQSLLVVGALSDVVEDQFARLLADLGIRDVAFLPPRRAAS
                     LPGVGPSMRFLLAQPFLAETGAALERRGARRIGALFPLGVDGTTDWLTAAARAFGIGW
                     ERIEAVLSAPARRAREAVARWRMDLEGRRIFFFPDSQLEVPLARMLARELGMVPLEVG
                     TPYLHREHLAAELAALPSGVALSEGQDVERQLDRCRALRPDVTVCGLGLANPLEAEGL
                     TTKWSIELLFTPIQGFEQAADLAALFARPLDRRSRLRV"
     gene            4691..>5504
                     /locus_tag="PK264_04075"
     CDS             4691..>5504
                     /locus_tag="PK264_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRD75106.1"
                     /translation="MQLTLWTYEGPPHVGAMRIATSMERVHYVLHAPQGDTYADLLFT
                     MIERRGTRPPVTYTTFQARDLGGDTAELFKTAAREAYERFRPDLLLVGASCTAELIQD
                     DPGGLAAALGLPVPVVALELPAYQRKENWGASETFYQIVRHLTAPAPAVSEAPQRRPG
                     QKPTCNILGPTSLGFRNRDDVTEVTRLLTGIGIGVNVVAPAGATPADIARLGAADFNV
                     VLYPEIAQAAAGYLKRRYGQPMTTIVPIGLGATGEFVAEVARLAGLEAQPGDA"
     assembly_gap    5505..5611
                     /estimated_length=107
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5612..6260
                     /locus_tag="PK264_04080"
     CDS             <5612..6260
                     /locus_tag="PK264_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRD75107.1"
                     /translation="ARDELGFTVAGIGSYSRELAREVRVAAAEHHVDALITDDYLAVE
                     RHIQVLQPELVLGTQMERHISKRLGIPCAVISAPVHVQDFPARYSPQMGFEGANVIFD
                     SWVHPLMMGLEEHLLGMFREDFEFNDGAAPSHLGHGKRIEVRSQPPESGRSAAPPATA
                     AGTPSWTSEAEKELTKIPFFVRGKARRNTERFASERGLPSITLETLYDAKAHFSR"
     gene            6235..9936
                     /locus_tag="PK264_04085"
     CDS             6235..9936
                     /locus_tag="PK264_04085"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit H"
                     /protein_id="HRD75108.1"
                     /translation="MQKHISAADQTPIRVVIITLDGHLAGVVDRVGRRLKATSYPGLE
                     LSFHIAADWHGDPAALSRCLEDIARGDIIVVTMLFLDDHIRAVLPALEARRAQCDALV
                     GCVAAGEIVKLTRIGRFTMDRQQGGALALLKRLRGSRKEGENSSSAASQMRMLRRIPQ
                     VLKYIPGTAQDMRAYFLAMQYWLPSDDNIEGLVRFLIERYADGPRRVSRRGGSVTEPV
                     VYHDVGLYHPRLTGRITDRLDDLPRNGNGAPTVGLLLMRSYLLAGNVAHYDAAIAALE
                     ARGLRVIPAFASGLDARPAIEAYFMRDGRPTIDALVSLTGFSLVGGPAYNDAKAAEAV
                     LARLDVPYIAALPVEFQSLDQWRGSLQGLLPIEATVMVAIPELDGATGSLVFGGRSLS
                     GDRASAHDMQPEPERISRLAERIERLIALRRTARAQRRVAIVLFNFPPNAGATGTAAY
                     LSVFESLHATLVALERAGYTVTVPPSVDALREAILDGNARRYGTSANVHARIPVDDHV
                     RREPWLADIERQWGSAPGRQQSDGAAIQVLGAAFGNVFVGIQPAFGYEGDPMRLLFER
                     GFAPTHAFSAFYRWMREDFGAHAVLHFGTHGALEFMPGKQAGLAADCWPDRLIASLPN
                     FYLYAANNPSEGTLAKRRSAATLISYLTPSVCQAGLYRGLAELKVTLNDWRQLAPADQ
                     AMRPELLALVQAQAVAVDLAKAEPAWTLQDLGAVESLGQAIVELEYTLIPHGLHVVGA
                     PMPAAEQRDLLAAIAEAGVAPGVDTATIATLAETGSVGRAALQKAPGLQSLIQVKTEL
                     DCNRETDAIVAALDGRFIRPAPGGDLIRSPEILPTGRNVHGFDPFRIPSAFALADGQR
                     QAERLLARHVEDGHGLPETVAIVLWGTDNLKSGGGPIAQALALIGARARFDGYGRLCG
                     AELIPLAELGRPRIDILATVSGIFRDLLPLQIKLLAEATYLAAAADEPVENNFVRKHV
                     LAYQAEQGCDLETAALRVFSNAEGAYGSNINHLVESSRWEDADELAETYSRRKGFAYG
                     RQGRPSPQPELLAKVLAGVDLAYQNLESVELGVTSIDTYFDTLGGIAGAARRVSPTGK
                     PPPVYIGDQTRGEGRVRTLGQQIALESRTRVLNPKWFEGMLKHGYEGVRQIEAHVTNT
                     VGWSATTGEVAPWVYRGISETFILDEAMRERLAALNPTASARMASRLIEAHDRQLWSA
                     DEATLAALREAGEELEDRLEGIPAKEAAA"
     gene            9933..10653
                     /locus_tag="PK264_04090"
                     /pseudo
     CDS             9933..10653
                     /locus_tag="PK264_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418673.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     assembly_gap    10654..10663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10664..10836
                     /gene="chlL"
                     /locus_tag="PK264_04095"
                     /gene_synonym="bchL"
     CDS             <10664..10836
                     /gene="chlL"
                     /locus_tag="PK264_04095"
                     /gene_synonym="bchL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255845.1"
                     /note="light-independent; with chlN(bchN) and chlB(bchB)
                     reduces ring D of protochlorophyllide to form
                     chlorophyllide a in chlorophyll/bacteriochlorophyll
                     production; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin:protochlorophyllide reductase
                     (ATP-dependent) iron-sulfur ATP-binding protein"
                     /protein_id="HRD75109.1"
                     /translation="RSRLKKSTLFEMPPTPALERVQQEYLALADRLWRGAEPLEARPM
                     KDREIFDFLGFD"
     gene            10836..11537
                     /gene="bchM"
                     /locus_tag="PK264_04100"
     CDS             10836..11537
                     /gene="bchM"
                     /locus_tag="PK264_04100"
                     /EC_number="2.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046406 - magnesium protoporphyrin IX
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium protoporphyrin IX methyltransferase"
                     /protein_id="HRD75110.1"
                     /translation="MPTATYIERRKELETYFDRTAVEAWEKLTSDAPVGRIRATVREG
                     RNDMRRTIVGWLPEDLSGRRVLDAGCGTGALAVELASRGADVLAIDLSPRLVELARER
                     YPARIGRGRVTFRVGDMLSRTPERFDHAIAMDSLIHYEAGDIAKALALLASQVEQSIT
                     FTFVPRTPLLVAMHSVGRLFPSSNRSPSFRPLGNNAARSLVAGERGLSCWHVARGHHV
                     SRGFYTSEAMELRRR"
     gene            11534..12967
                     /locus_tag="PK264_04105"
     CDS             11534..12967
                     /locus_tag="PK264_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HRD75111.1"
                     /translation="MIGRGHRIATLWSRLGPRFLPFADSASAELPLGRLLRLSLFQVS
                     IGVALVLLNGTLNRVMIVELGVSASIVAAMIAIPVLLAPLRLLLGYRSDYHRSFLGWR
                     RVPYIWGGTMLQFGGLAIMPFALIVLSGDANGPAFVGPLAAAMAFLMVGAGMHTTQTA
                     GLALASDLAAPEKRHRVVALLYVMLLVGTFFASLAFAWLLASFSQVRLIQVIQGAALL
                     TMTLNLVALWKQEARQPALTRHDQIQPAFRETWREIRSDRRCLRLMVAVALGTAGFGM
                     QDILLEPYGGEILGLSVAGTTVLNSLVALGTLAGFAIAARWLDRGGDPMRLAAAGLLV
                     GIVAFSAVVFASPLRSPGLFRFGATLIGFGTGVFAVGTLTSAMQHVRHGLGGQILGAW
                     GTAQAIALGLALAASGLIRDLTSSLAAGGHLGPALRGPESGYLVVYHIEIGFLFAALV
                     AIGPMVRLSATTMSERDGSFGVAEHPS"
     gene            12985..13779
                     /gene="puhA"
                     /locus_tag="PK264_04110"
     CDS             12985..13779
                     /gene="puhA"
                     /locus_tag="PK264_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit H"
                     /protein_id="HRD75112.1"
                     /translation="MPKGSITSYIDVAQVTLYLFWFFFAGLIFYLLRENKREGYPLDS
                     DRGGNIKVQGFPAIPVAKTYLLPHGGTQTAPRDERDSRTLQAKPSAPWPGAPLVPTGD
                     PMRDGVGPAAWAERADKPDLSVDGHNRIVPLRVATGFHLETRDPNPIGMAVVGADGLV
                     GGTVADVWVDRSESLIRYLEVKLPAAAGAAPADAARTVLLPMTFARVAGNRRLVHVRS
                     ILASQFAGVPAISTPDAVTRREEDRICGYFGGGTLYATPSRQESLL"
     gene            13776..14468
                     /gene="puhB"
                     /locus_tag="PK264_04115"
     CDS             13776..14468
                     /gene="puhB"
                     /locus_tag="PK264_04115"
                     /inference="COORDINATES: protein motif:HMM:NF040894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex putative assembly protein
                     PuhB"
                     /protein_id="HRD75113.1"
                     /translation="MTHDDFAHEPIRGLPERPPAGEEVLWQGAPHWPTLARRVMHIDL
                     VAAYFLAIGAWKVANGVATGLPGTEIAVSLAAFAALSILAVGVLALLAWGIGRTTVYT
                     ITSRRVAMRFGIALPVTFNLPFSVINSAGVRDLGGGRGDLALELGAGQRIAFLVLWPH
                     VRPWRLRTPEAMFRAVLYVARVSGILSTALKVELQRTGVRTVASSVRQGREPEAVISH
                     QPIARPALTAAE"
     gene            14485..14943
                     /gene="puhC"
                     /locus_tag="PK264_04120"
     CDS             14485..14943
                     /gene="puhC"
                     /locus_tag="PK264_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic complex assembly protein PuhC"
                     /protein_id="HRD75114.1"
                     /translation="MHDNAKPWGQPGWMLASASLLVVAAISLAAIARYSGIGASRIGE
                     PHAVRVIDLRFEDQAAGRILVTDVSGRLPPRMIEPGKDGFVRVALRSLARDRALIGIG
                     SEVPFRLGEQADGSIWLRDLATGRLLLLDAYGHANRTSFSQLLAHAGATP"
     gene            14940..15242
                     /locus_tag="PK264_04125"
     CDS             14940..15242
                     /locus_tag="PK264_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75115.1"
                     /translation="MIRLLRPRTQFDIPCTIEIENTFESLHAHVELDGDVAVEAGDEV
                     LVHGDTIRVPYGQKMTLRRQATVRRASGIDRLLTKLAARFELTELYEVSFSSGRKL"
     gene            15239..16302
                     /gene="acsF"
                     /locus_tag="PK264_04130"
                     /pseudo
     CDS             15239..16302
                     /gene="acsF"
                     /locus_tag="PK264_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0048529 - magnesium-protoporphyrin IX
                     monomethyl ester (oxidative) cyclase activity [Evidence
                     IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     (oxidative) cyclase"
     gene            16324..17268
                     /gene="puhE"
                     /locus_tag="PK264_04135"
     CDS             16324..17268
                     /gene="puhE"
                     /locus_tag="PK264_04135"
                     /inference="COORDINATES: protein motif:HMM:NF023711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative photosynthetic complex assembly protein
                     PuhE"
                     /protein_id="HRD75116.1"
                     /translation="MMLDASPIAIAACLVWWASTGAILSLIGRGESTYRWSMLGAALL
                     GVAAIAAVVASSRATTTQGAFIAFIAAIGIWGAIEMSFLMGFVVGPRRGPCPPGLTGW
                     QRFKVSFAALAHHELLLVAALAALAWLVADQPNRVALYTFALLWVMRLSTKLNIFLGV
                     SNVSEEVLPARIGYLKSYFRKAPMNALFPLSVTGATACVFYFGGLAASPGASDFERAS
                     AALLATFSALALIEHWMLVLPLATAAIWPWAPAPERSAGSLAIGHPTNAAHAGSDPAA
                     TGDRPHVRPQVISSAVGRGASPVEATIVDIPRQPWRTK"
     gene            17265..18497
                     /gene="hemA"
                     /locus_tag="PK264_04140"
     CDS             17265..18497
                     /gene="hemA"
                     /locus_tag="PK264_04140"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HRD75117.1"
                     /translation="MNYEAQFESYLNSLHSEGRYRVFADLMRKCNEFPRAVRYKAGSE
                     SNVTVWCSNDYLGMGQSRIVLDAMHEALDRCGAGSGGTRNISGTNHYHIELEAELADL
                     HRKEAALVFTSAYVANDAAISSLVKLLPGAVILSDEKNHASMIAGIRHGGGEKHIFKH
                     NNLVSLESQLKALPRDVPKIIAFESVYSMDGYISPIGAICALAKKYGALTYLDEVHAV
                     GMYGPRGGGIAERDGVLDQVDIINGTLAKGFGVMGGYIAASRSCCDAIRSFAPGFIFT
                     TSLAPALAAGALASIRHLKVSMLERRRHQERAGYVKRALGEAGLPVLENPSHIVPLMV
                     CNPVHCKLISDALLDAHGVYVQPINYPTVPRGTERLRITPTPLHSDADVDHLVASLKA
                     LWETCPMSSTASLQHAAE"
     gene            18554..20485
                     /gene="dxs"
                     /locus_tag="PK264_04145"
     CDS             18554..20485
                     /gene="dxs"
                     /locus_tag="PK264_04145"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRD75118.1"
                     /translation="MSDFSTTPLLDKVQFPEDLRALPDRELRQVADELRKETIAAVSI
                     TGGHLGAGLGVVELTVALHHVFDTPSDRIIWDVGHQAYPHKILTGRRDRIRTLRQGGG
                     LSGFTKRSESEYDPFGAAHSSTSISAGLGMAVARDLTGGTNNVVCVIGDGALSAGMAY
                     EAMNNAGARDARLIVILNDNDMSIARPVGALSSYLARLTTSGAYLQFRDALKQLARRL
                     PKAWERRAARAEEYTRTYWTGGSLFEELGFYYVGPIDGHDLDQLLPVLRNARQAKHGP
                     ILIHVVTKKGKGYAPAETASDKYHGVNRFNVVTGEQVKTPANAPSYTRVFAQGLIREA
                     EFDPRIVAVTAAMPSGTGLDLFGRRFPDRTFDVGIAEQHAVTFAAGLATEGMKPFVAI
                     YSTFLQRGYDQIVHDVALQRLPVRFAIDRAGLVGADGATHAGSFDVAYLGNLPGMVLM
                     AAADEAELMHMVATAARVDDRPSAFRYPRGDGVGLELPERGTPLEIGRGRIVREGSAV
                     ALLSYGTRLSECMTAAGQLAAMGLSATVADARFAKPLDKALVDQLVRHHEVLITVEEG
                     SVGGFGSFVLQHLAASGRLDRGLKVRTLVLPDEFIDHDKPEVMYRKAGLDAASIVGAV
                     CHALEIDRPARASRLQSGQ"
     gene            20497..>21251
                     /locus_tag="PK264_04150"
     CDS             20497..>21251
                     /locus_tag="PK264_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRD75119.1"
                     /translation="MTSAGESPIASPDLEIRRQKSGGERRTALGEHDGPSDIARRKSE
                     HVEVVLHGNVGARNATTGLEKVRFEHCALPELSLDDIDLSTRLAGRPLAAPLIVSSMT
                     GGPLHARRINYAIAEAAGRAGIGFAVGSQRIALEGRASAGFSRKLRSLAGNVPILANF
                     GGAQLRHWSGTLEAERAIEMIDADALIIHLNPLQEAVQHGGDRDWTGLLRRIEELARD
                     TSRPLIVKEVGFGISGRVARQLWNAGVTIIDVAG"
     assembly_gap    21252..21338
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21339..21631
                     /locus_tag="PK264_04155"
     CDS             <21339..21631
                     /locus_tag="PK264_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HRD75120.1"
                     /translation="GVPTARAITDVREACPQVTIIASGGIRDGLDVARAIRLGADFAG
                     QAAGILPAALNGADAVAEHIAILVEQLRIACFCTASADLAALRRAPLLSGAD"
     gene            21734..23299
                     /gene="crtI"
                     /locus_tag="PK264_04160"
     CDS             21734..23299
                     /gene="crtI"
                     /locus_tag="PK264_04160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HRD75121.1"
                     /translation="MPSARPADAGSCGDGAEPLPTAIVVGGGIGGMAAALRLRSKGWA
                     VTLIERQAELGGRAQVYIHDGFRHDAGPTIITAPFLIDELFELFGRRREDYLRLVPLA
                     PWYRFHFHDGTTFDYGGTIEDTLAEIRRIEPRDVEGYRCLLEHSRSLYDIAFTKLATR
                     PFDSLSMLLAQTPRLARLRADRSVWKLIARHLSNDKLRRAFSIQPLLVGGNPFTTTSI
                     YALIHYLERRSGVFFAMGGTGALVTALRRLLAETGVIVRTATTVEKVLVERGEARGVR
                     LADGEVIRSRVVVSNADPMHLYGHMIDREHIRLTTRAKLASRLSMGLFILYFGTRRTY
                     PAVAHNTIWLGERYKELLADVFRNGVLADDFSLYLHRPTATDPSFAPPGCDSFYALCP
                     VPNNAAGISWRVEGPKLRSRVIAALDRTILPGLSESITADFFVTPDDFADRFLAVEGS
                     GFSVAPYFLQSAWFRFHNRSESIRNLHLVGAGTHPGAGIPGVLCSARIAADQIGEAAV
                     GSVAVSELAAGIR"
     gene            23431..24300
                     /locus_tag="PK264_04165"
     CDS             23431..24300
                     /locus_tag="PK264_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD75122.1"
                     /translation="MDPRERVELALESSVMGASRDGCPPGLAAAVRHAIFPGGARVRP
                     LLCLGVSVACGDGAPKAADAAAAAIELLHCASLVHDDLPCFDDADLRRGKPSVHRAFG
                     EPIAVLTGDALIVLAFETMAMACRNAPRMLPDLVSILSKAVGMSGGLAAGQAWESEAN
                     VPLATYHGAKTGSLFVAATKAGALAAGADPLAWEEFGSRLGEAYQIADDIRDACSTSA
                     ELGKPVGRDVALGRPSAIRNFGLEDAFKHLDGLVDSALSAVPACPGAAELKSIVRRQV
                     RWFVPKESALHAA"
     gene            24499..25476
                     /locus_tag="PK264_04170"
                     /pseudo
     CDS             24499..25476
                     /locus_tag="PK264_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951054.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            25473..>26063
                     /locus_tag="PK264_04175"
     CDS             25473..>26063
                     /locus_tag="PK264_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75123.1"
                     /translation="MTALRESGDRARRASDAALAAILLAVAPRELGGASIRLAAGSVA
                     EAWIDLAVPEVPGRPRPVRLPIGLTNEQLAGGVDLAATLAVGRKVMQPGLIERNKGCA
                     LIVPSAERWSAEAAACLVGRIDQRCDADADGPTEGHLLLALDEGCGEDERPPELLCDR
                     LAFLIDLAGLREADVAGPTDLTDRLEAARALHPNVAV"
BASE COUNT         4150 a         8607 c         8465 g         4637 t
ORIGIN      
        1 ccgagctctc ggctcgtgtt aacgaacttc gcgatcggga cactagcgac ggccgcggcg
       61 tccgcgccgg aagtggggtg agccgcttgg atatcggttc acatgccaat tcctgattgc
      121 ctggggtcaa tcgccactag ggtcgttcag cccataacgg cgcatcttca tataaaggct
      181 ctggcggctg agacccagca tttcggctgc ggaggcccga ttgtcgtcga cgatctgcag
      241 tgcggtctcg atgcacattc tctcgatcag gtcggttgcc tcgcggacga gattcttgag
      301 gggcacctgc cctacgagac ctgcgagttg actggcggac cgcggcagcg actggctcaa
      361 cgtaccctcg gcggcgagct ggcggccgat gctcctgatc gtgaaaccga agcaaggctg
      421 ctcgccgctc tccaccgtca cggcggatac ctcgacatcg tcggagctgc cgagctcgcc
      481 ctggaccaca gtccggaagc tgcgcaccga gccgtgctcg cgcaggcttc cgatcagcag
      541 gttggtctcg gcgggcgtgc ggccgaccca gcgatccagg ggctggcccc ggatctgctc
      601 gagactgctc atctgagcaa gctccaggaa tgcggcgttc gccatcagga cgcgacggtc
      661 gaggccggag acgacgaacg cgtctggcat gctctcgacg agcgcacgca gtgtctcgcc
      721 gctcgatggg gcgtccgtgc gctccgtcac gtcggccggg acaagccgca gcagcagatg
      781 ggccgtggcc tcctgcttga agagagaccc cgtgacaacg acctcggtac gagcattggc
      841 gaggcgcgac gtcagcgggt cggcgcgccc ggaggtccgc agcccgtcga gcatccgctg
      901 gacggcaccc tggctcgagg attggaacag ctcgacgact gtcttgccgg agatgcggcg
      961 agcggccggc tcgaggaggt gctgggccgc gggattggcg tcgctgatcc gcaaggtcgc
     1021 cgcgtcggca atgatgacgg cgtcgccagc aatctggaac agcagacgaa agcgcgtctc
     1081 tgcgctccga actcgtgcat gttcgcgctc gaccgcctgc tgtgtctcga ggagcttctg
     1141 ctgcagaagc gacatcgagc gcagctcgcg gccgagggcc aggatgcggc cgccttgccc
     1201 gatccgcatg gtcgcgtagc gaaccggcag gtcgccacgg ccattgggat ttggatggtt
     1261 cacctggcgc cactgagcgc cttggcgtgc aatggttgag gcgtcactca gaagctgcgc
     1321 aatcttgggc cggctctcga tcgtgaccgt atcgatccag ttccgcccga tccaggaatg
     1381 gacgtcagat tccttggaca aactgtcgct gcaaatggcc acgtcacaga cggttccgtc
     1441 cgggtccatc atcacggcga ggtcggtcgc gacggtcacg agacgtgtgg ccgcttccgc
     1501 gttgagagag ctgatcgctt tcctgaggct tcgcacgttg ccgaccgctc attcaaggtg
     1561 cacgtatggc catacgcaga gccaccaatc agtgccaatc aaacgttctc gacctattgg
     1621 cctgcactgt aggccattcg ccaaggcgat cagcccgcca ccaacctctc agccgccagg
     1681 agggccgagc gtgcatcgct cgccgatcca tccgctccca gtcttgcgac cacgtttgga
     1741 tcggtttcga gagcgcgccc gccgacaagc acctgcagac gtggattgat gcttgcctgt
     1801 cgcatccggt caatatccga tgcggcctgc cctagcaagc tctcgcggct gatggacatg
     1861 ccgaccacgt cggtcgcatg ccgcctcaag cgggcacaga ctgcgccgat cttcagctcg
     1921 aagtgcgcct ccacgatcca gccggccatg gtgaagaagg catcgagaat gctcagcgga
     1981 aacgtgtgct gctcgccggc agcagggacc agtaggatgg accggccctt gagcgttccc
     2041 tgccgcgcgg accggcccga tgcctcgtcg agatggtgca tggctcgttg cagctcgccc
     2101 atggccatgg tgacctcggc aaaagtccgc tcgtcgctct cccacaactc gccaagtcgg
     2161 cgcgccgccg gcgcaaagag ttcaagcagt acctgtgcga cgctggcgcc ctgcaccagg
     2221 aaggcgtcca caagctctgc tacacgcgcc gtatcgctgt cgaggagcgc cgtgcagagt
     2281 gttgccacat cctcgtcgat aatggtgatc cgcccgcttg cgcgctcacc gagctgtgca
     2341 gcttgttgac gggtcagcaa cataaggcgc ggcgcgatct cggtctcgat ggcgcatcga
     2401 agcagcattt cctggacaat cgtgtcacga ggactcgaca gacgtgtttg taacgagcct
     2461 cttatgtcgg gccgcgtacc gtcccccgga cgtgactctt cacgacagcc ggatgaaggt
     2521 ccgttccaac gcgtcaaagt gaccatgccg gcccctcctg actctggcgc ggagtgacgc
     2581 tgcactcccg ttcactcccg gtgcggcgtg atgctgcgac ctgctgcgcc gcccgcaatc
     2641 atttgccgct tgcacatcgc ttgtagaccg cgacttgctt gcccagcctt gccgagccgg
     2701 caggatttcc tgcgcccccc atcggctgat aactcagtga gccatgcgac gactcgacgc
     2761 gataggtgta gttctacgca aatagtaaaa ttgacttgac gtaaaatatt attgacggtc
     2821 tcatgccctc gagaagcacc cgagggtgcg atcgcctgag gggtcccacg atgcgcgacg
     2881 cgcctcttag caagcctcgg agccaagctc tctactcggt tgatgagcgg cggcgccgcg
     2941 atgcctcccc atggacgatc gttcagggcg tcctcgcccc ggtccagttt gtgatcttcc
     3001 tcgtctccgc cgcactcgtg cttcggttcc tcgcgaacgg cgaaggttac gtcgctgcca
     3061 cggcttcgat cgttctgaag acgctcgcgc tttacgcgat catgatcacc ggctcgatct
     3121 gggaaaaggc cgtgttcggt cgttatctct ttgcgccggc atttttctgg gaagacgtcg
     3181 tcagcatgct ggtgctggca ttgcatacgg cgtacctcgt cgtcctcgtg ctcggtctca
     3241 tgccggcgac ggggcagatg atcctcgctc ttctggccta cgcgagctac gtcatcaatg
     3301 cgggccagtt cctcctgaag ctccgccgcg cacggatcga gcagccgcag gacgccctcg
     3361 cgcgcgtgcg cctcggaggg gaggcgctcg gatgatgccc tccgccgcac ttgccccagc
     3421 agcctttgcc gaaagcagcg gcagtcagcg ctacgtgctt cgcgagcgag gccagcgcga
     3481 ggtcttctgc ggcctcaccg gaattgtctg gctgcaccgc aaaatccagg atgccttctt
     3541 tctggtcgtc ggatcgcgga cgtgtgcgca ccttctccag tccgcggctg gcgtgatgat
     3601 cttcgccgag ccgcgctttg ccacggcgat catcgacgag cgcgaccttg ccggccttgc
     3661 cgatgccaac gacgaactcg accgggtcgt cgccgaactg ctggcgcgcc gcaccggcat
     3721 caagctgctg ttcctggtcg gctcgtgccc gtccgaggtg atcaagctcg acctcggcaa
     3781 ggcggccgag cggctgtcgc gccgccacgc tcctgcgaca cggatcctgc actattcggg
     3841 cagcggcatc gagaccacgt tcacgcaggg cgaggacgcc tgccttcagg cgctggtcgg
     3901 cgcgatgccg gcgacagccg ctgattcgcc ccagagcctg ctcgtcgtcg gcgccctgtc
     3961 ggatgtcgtc gaggaccagt tcgcgaggct gctcgccgac ctcggcatcc gggacgtggc
     4021 attccttccg ccgcggcgcg ctgcgagcct gccgggcgtg gggccgtcga tgcgcttcct
     4081 gctcgcgcag ccgttcctcg ctgagaccgg ggcggcactg gagcggcgtg gcgcgcggcg
     4141 catcggcgca cttttccctc tcggcgtcga cggcacgacg gattggctca cagccgcagc
     4201 ccgtgcattc ggaataggct gggagcggat cgaggcggtg ctgagtgcgc cggcccgtcg
     4261 tgcccgcgag gcggtcgcgc gctggcggat ggatctcgag gggcgacgca tcttcttctt
     4321 ccctgacagt cagctggagg tgccgctcgc gaggatgctc gcgcgggagc tcggcatggt
     4381 gccgctcgaa gtcggcacgc cgtatctgca ccgcgagcat ctggccgccg agctcgcggc
     4441 tctgccgtcg ggcgtagcgc ttagcgaggg ccaggatgtc gagcgccagc tcgaccgctg
     4501 tcgcgccctg cgtcccgacg tcaccgtctg cggcctcggg cttgccaatc cgctcgaggc
     4561 tgaggggctt acgacgaagt ggtctatcga gctgctgttc acgccgatcc aagggttcga
     4621 gcaggccgcg gatctcgccg cgctcttcgc ccggccgctc gatcgccggt cacggttgag
     4681 ggtctaggcc atgcaactca ccctctggac atatgaaggc cctccgcacg tcggcgcaat
     4741 gcggatcgca acatcgatgg aacgagtgca ctacgtgctg cacgcgccac agggcgacac
     4801 ctacgctgat ctgctcttca cgatgatcga gcggcgcggc acccgtccgc ccgtcaccta
     4861 tacgaccttc caggcccgcg atctcggcgg cgacaccgct gagctcttca agaccgcggc
     4921 tcgggaagcc tacgagcggt tccgaccgga cctgctgctc gtcggcgcct cctgcaccgc
     4981 ggagctgatc caggatgacc ccggcggtct cgccgcagcg ctcggcttgc ccgttccggt
     5041 cgtcgcgctc gagctgccag cctaccagcg caaggagaac tggggcgcct cggagacctt
     5101 ctaccagatc gtccgccacc tgacggcgcc agcacccgct gtgagcgaag ccccgcaacg
     5161 ccggccgggg cagaagccga cgtgcaacat cctcgggccg acatcgctcg gcttccggaa
     5221 ccgcgatgat gtgacggagg tgacgcgcct cctcaccggg atcgggatcg gcgtcaacgt
     5281 cgtggcgccc gccggagcga cacccgctga catcgcgcgt ctcggtgcgg cagacttcaa
     5341 tgtcgtcctc tacccggaga tcgcgcaggc ggcggccgga tacctgaagc gtcgctacgg
     5401 ccagccgatg acgacgatcg tgcccatcgg ccttggcgcg acgggggaat ttgtcgccga
     5461 ggtcgcccgg ctcgccggcc tcgaggcgca acccggcgac gcggnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcccgcga cgagctcggc ttcaccgtcg
     5641 ccggtatcgg cagctacagc cgtgagctgg cccgtgaggt gcgtgtggct gcggccgagc
     5701 atcacgtcga tgcgctgatc accgacgact atctcgcagt cgagcgccat atccaggtat
     5761 tgcagcccga actcgtcctc ggcacgcaga tggagcgcca tatctcaaag cgcctcggca
     5821 ttccgtgtgc cgtcatctct gcgcccgtgc atgtgcagga tttcccggcc cgctactcgc
     5881 cgcagatggg tttcgagggc gccaacgtca ttttcgacag ctgggtgcat ccgctcatga
     5941 tggggctcga ggagcacctc ctcggcatgt tccgcgagga cttcgagttc aatgacgggg
     6001 cggcgccctc ccacctcggg catggcaagc ggatcgaggt tcgatctcag ccgccggagt
     6061 ccgggcggtc ggctgcgccc cctgccaccg ctgccggcac accatcctgg acgagcgagg
     6121 cagaaaagga gctcacaaag atcccgtttt tcgtgcgcgg caaggcccgc cgcaacacag
     6181 agcgcttcgc cagcgagcgc ggtctgccat cgatcaccct cgagacgctc tacgatgcaa
     6241 aagcacattt cagccgctga ccagacgccg atccgcgtcg tcatcatcac gctcgacggg
     6301 catcttgccg gtgtcgtcga tcgggtcggc cgacggctga aggcgacgag ctacccgggg
     6361 ctcgagctct cgttccacat cgctgcggat tggcacggtg atccggcggc cctctcccgc
     6421 tgcctcgagg atatcgctcg cggcgacatc atcgtcgtca cgatgctgtt cctggacgac
     6481 catatccgtg cagttctacc ggcactcgag gcgcgtcggg cgcagtgtga cgccttggtc
     6541 ggctgcgttg ccgccggcga gatcgtcaag ctcacgcgga tcggccgctt cacgatggat
     6601 cgtcagcagg gcggtgcgct cgcattgctg aagcggttgc gcggctcgcg caaggaaggc
     6661 gaaaactcga gctcggcggc gtcacagatg cgtatgctgc ggcgcatccc gcaggtgctg
     6721 aagtacatcc ccggcaccgc gcaggacatg cgtgcctact ttcttgccat gcagtactgg
     6781 ctcccctcgg acgacaacat cgagggcctg gtccgcttcc tgatcgagcg gtatgccgac
     6841 ggcccgagac gggtctcccg acgcggcggc tctgtgaccg agccggtcgt ctatcacgac
     6901 gtcggcctct atcacccgcg cctgaccgga cggatcacgg accggctcga cgatctgccg
     6961 cggaatggca atggcgcgcc gacagtcggc ctcctgctga tgcggtccta tctgcttgcc
     7021 gggaatgtcg cgcactacga tgccgccatt gccgcgctcg aggcgcgcgg cctgcgcgtc
     7081 attccggcgt ttgcgagtgg cctcgacgcg cggcccgcga tcgaggcgta cttcatgcgc
     7141 gacggtcgtc cgacgatcga tgcgctggtc tcgcttaccg gcttctcact tgtcggcggc
     7201 ccggcctaca atgatgccaa ggccgccgag gcagttcttg cgcgtctcga tgtgccttat
     7261 atcgcggcgc tgccggtcga gttccagtcg cttgaccagt ggcggggctc gctccagggc
     7321 ctgctgccga tcgaggcgac ggtgatggtc gccatccccg agcttgatgg ggcgaccggc
     7381 tcgctcgtgt tcggcggccg atcactcagt ggcgaccggg catccgccca cgacatgcag
     7441 cccgagccgg agcggatcag tcggcttgca gagcgcatcg agcgcctgat tgcattgcgc
     7501 agaaccgcgc gcgcgcagcg ccgcgtggcg atcgtgctct tcaatttccc gccgaatgcc
     7561 ggcgcgaccg ggacggcggc gtatctctct gtcttcgagt cgcttcatgc cactctcgtc
     7621 gcactcgagc gggccggcta taccgttacc gtgccgccct ccgtcgatgc gttgcgcgag
     7681 gcaattctcg acggcaacgc ccgccgctat gggacctccg ccaacgttca tgcgcgcatc
     7741 cccgtcgacg accatgtgcg tcgtgagcct tggcttgccg atatcgagcg tcagtggggc
     7801 agcgcgcccg gacgccagca gagcgacggc gcggcaatcc aggttctcgg cgcagctttc
     7861 ggcaacgtct tcgtcggcat tcaacctgcc ttcggctacg aaggtgaccc gatgcggctt
     7921 ctcttcgagc ggggattcgc gccgacgcac gccttctcgg ctttctaccg ctggatgcga
     7981 gaggatttcg gcgcgcatgc cgttctgcat ttcgggacgc acggcgcgct cgagttcatg
     8041 cccggcaagc aggccggcct tgcggcggac tgctggccgg accggctgat cgccagcctg
     8101 ccgaacttct atctctacgc tgccaacaac ccgtcagagg gtacgctcgc caagcgccgc
     8161 tcggccgcga cccttatcag ctacctgacg ccatcggtct gtcaggccgg gctctatcgc
     8221 ggcctcgccg agctgaaggt gacactcaac gactggcggc agcttgcgcc ggccgaccag
     8281 gccatgaggc cggagctgct ggcgctcgtc caggcgcagg cggtggcggt cgatcttgcc
     8341 aaggcggagc cggcctggac gctgcaagac ctaggtgccg tcgagagcct ggggcaggcg
     8401 atcgtcgagc tcgagtacac gctgattccg catggcctgc atgtcgtcgg tgcgccgatg
     8461 ccggcggccg agcagcgcga cctcctcgcc gccatcgccg aggcgggcgt cgcgccgggc
     8521 gtcgacacgg caactatcgc aacgctcgcc gaaacgggtt cggttggtcg ggcggccctg
     8581 cagaaggccc ccgggctcca gtcgctgatc caggtcaaga ccgagctcga ttgcaaccgc
     8641 gagaccgacg caatcgtcgc ggcgctcgac ggcaggttca tccgcccggc gcccggaggc
     8701 gacctcatcc gctcgcccga gatcttgccg acgggccgca atgtgcacgg cttcgacccg
     8761 ttccggattc cgagcgcctt tgccctcgcc gacggccagc ggcaggccga gcggctcctc
     8821 gcgcgccatg tcgaggacgg ccatgggctg ccagagacgg tcgcgatcgt cctctggggc
     8881 acggacaacc tcaaatccgg cggcggtccg atcgcacagg ctctggcgct catcggcgcg
     8941 agggcgcgct tcgacggata tggccggctc tgcggcgccg agctcatacc gcttgccgag
     9001 ctcgggcgac cgcggatcga catactcgcg accgtatccg gtatcttccg tgatctgctg
     9061 ccgctgcaga tcaagttgct cgcggaagcg acgtatctcg cagccgccgc cgacgagccc
     9121 gtcgagaaca atttcgtgcg caagcatgtg ctcgcctatc aggcggagca gggctgcgac
     9181 ctcgaaacgg cggcgctgcg ggtcttcagc aatgccgagg gcgcatacgg ctcgaacatc
     9241 aaccatctgg tcgagagcag ccgctgggag gatgccgacg agcttgccga gacatactcg
     9301 cgccgcaagg gtttcgccta tggtcgccag ggccggccga gccctcagcc tgagctgctc
     9361 gccaaggtcc tcgccggcgt cgacctcgcc tatcagaacc tcgaatccgt tgagctcggc
     9421 gttacatcga tcgacaccta cttcgatacg ctcggtggaa tcgccggggc tgcgcgccgc
     9481 gttagcccaa ccggcaagcc accgccggtc tacatcggcg accagaccag gggcgagggg
     9541 cgcgtccgca cgctcggcca gcagatcgca ctcgaatccc gcacacgcgt cctgaacccg
     9601 aagtggttcg agggcatgct gaagcacggc tacgaaggcg tgcgccagat cgaggcacac
     9661 gtcaccaata ccgtcggctg gtcagccacc accggcgagg tcgcgccgtg ggtctatcgc
     9721 ggaatttccg agaccttcat cctcgatgaa gcgatgcgcg aacggctcgc cgctctcaac
     9781 ccgaccgcct ccgcccgcat ggcgagccgg cttatcgagg cgcacgatcg ccagctgtgg
     9841 tccgccgacg aggcgacact tgcggctctg cgtgaggccg gcgaggagct cgaggatcgg
     9901 ctcgaaggaa ttcccgccaa ggaggctgcc gcatgaccac ggtgctcgat gcacggccga
     9961 gaagccgccc tccggctgga cgcgatgacg gtgagggaag cgtgcaggtt cgcctggatc
    10021 ccaagctcca gatcgccacc aaggcgaagg ttttcgctgt ctatggcaaa ggcggcatcg
    10081 gcaagagcac gacgtcgtcg aacctctccg tcgcgttctc gaaaatcggc aagcgcgtcc
    10141 tccagatcgg ctgcgacccg aagcacgatt cgaccttcac cctgaccaag cggctgatgc
    10201 cgacggtcat cgacgtgctc gagcagatca attttcacac cgaagagctc aggccggaag
    10261 atttcgtgtt cgaaggctac aacggcgtgc tgtgtgtcga ggcgggtgga ccgccggcgg
    10321 gcaccggctg cggcggctat gtggtcggcc agacggtgaa gctcttgaag gagcaccacc
    10381 tcctcgacga gaccgacgtc gtcatcttcg acgtgctcgg cgatgtcgtc gccgcttcaa
    10441 catgccgaac gcggcctgat cgtcacggcg aacgacttcg attcgatctt cgccatgaac
    10501 cgcattgtcg ccgccatcca ggcgaaggcc aagaactacc gcgtcaggct cggcggcgtc
    10561 atcgcgaacc gcagtgccgg caccgacgag attgaccgct acgcaacggc gaccgggctc
    10621 gcgcggatcg cccacttccc cgatctcgac gaannnnnnn nnngtcgcag ccggctcaag
    10681 aagtcgaccc tcttcgagat gccgccgacg cccgccctcg agcgggttca gcaagaatac
    10741 ctcgcgctgg ccgacaggct ctggcgaggg gccgagccgc tcgaggcgag gccgatgaag
    10801 gaccgtgaga tcttcgattt cctgggattc gactgatgcc gacagcaact tacatcgaac
    10861 ggcgcaaaga gctcgagacc tacttcgatc gcaccgcggt cgaggcttgg gagaagctca
    10921 ccagcgacgc gcccgtgggg cggatccgtg cgaccgtccg cgagggccgc aatgacatgc
    10981 ggcggacgat cgtcggctgg ctgccggaag acctctcggg ccgccgtgtg ctcgatgcgg
    11041 gctgcggcac gggggctctt gcagtggagc tcgcctcgcg cggcgccgat gtgctcgcga
    11101 tcgacctctc gccacggctg gtcgagcttg cccgcgagcg ctatcccgcc cggatcggcc
    11161 gcggccgcgt cacattccgc gtcggtgaca tgctgagccg tacgcccgag cgattcgatc
    11221 acgcgatcgc catggacagc ctgatccact acgaggccgg cgacatcgcc aaggcgttgg
    11281 cgctgctggc atcgcaggtc gagcaatcga tcaccttcac gttcgtgccc cgcacaccgc
    11341 ttctcgtcgc catgcattcc gtcggccgcc tgtttcccag cagcaatcga tccccgtcgt
    11401 tccgtccact cgggaacaac gctgcgcgca gcctcgtcgc gggcgagcgc ggcctctcct
    11461 gctggcacgt ggcccgcggc caccacgtct cgcgcggctt ctacacctcc gaagccatgg
    11521 agctgagacg gcgatgatcg ggcgcggcca caggattgcg acgctctgga gccggctcgg
    11581 cccacgcttt ctgccgttcg cagacagcgc ctcggccgaa ctgccgctcg gccggctgct
    11641 gcgactgtcg ctcttccagg tctcgatcgg tgtggctctg gtgctgctca atggcacgct
    11701 caatcgcgtg atgatcgtcg aactcggcgt ttcggcatcc atcgtcgcgg cgatgatcgc
    11761 cattcccgtg ctgctcgcgc ccctgcgact gctcctcggc taccggtccg actatcaccg
    11821 ctccttcctc ggctggcggc gcgtccccta tatctggggc ggcaccatgc tgcagttcgg
    11881 cggcctcgcc atcatgccgt tcgcgctcat cgttctgtcg ggcgatgcga acggacccgc
    11941 gttcgtcggg ccgctcgccg cggcgatggc cttcctcatg gtcggcgcgg gcatgcacac
    12001 gacccagacc gcgggcctgg cgctggcgag cgacctcgcc gctcccgaga agcgccatcg
    12061 ggtggttgcg cttctctatg tcatgctgct tgtgggcacg tttttcgcct cgctcgcgtt
    12121 cgcatggctt ctggcgagct tctcgcaggt gcgcctcatt caggtgatcc agggcgccgc
    12181 gctgctcacg atgacactga acctcgtggc gctctggaag caggaggcgc ggcagccggc
    12241 gctgacgcgg cacgatcaga tccagccggc attccgcgag acgtggcggg agatccgctc
    12301 agaccggcgt tgcctcaggc tcatggtggc ggtcgcgctc ggtacggccg ggttcggcat
    12361 gcaggacatc cttctcgagc cctacggcgg cgaaatcctc ggtctctccg ttgcaggcac
    12421 gaccgtgctg aactccctcg tcgcgctcgg cacgctcgcc ggcttcgcca tcgcggcgcg
    12481 ctggctcgat cgcggcggcg atcccatgcg gctcgcggcg gccggactcc tcgtcggcat
    12541 cgtcgcattc tcggcagtcg ttttcgcctc gccgctgcgc tcgcccgggc tgttccggtt
    12601 cggtgcgacg ctgatcggct tcgggacggg cgtgttcgcc gtcggcacgt tgacgagcgc
    12661 gatgcagcac gtcaggcacg gcctcggcgg tcagatcctc ggcgcctggg gaacggcgca
    12721 ggcgatcgca cttggcctcg cgctcgcggc gagcggcctt atccgcgacc tcacgtcgtc
    12781 gcttgcggcc ggcggtcatc ttggccccgc gctgcgcgga cccgagagcg gctacctcgt
    12841 cgtctatcac atcgagatcg gcttcctctt cgcggcgctc gttgccatcg gcccgatggt
    12901 ccggctgagc gccacgacca tgtcggagcg ggacggaagc ttcggtgttg ccgagcatcc
    12961 aagctaactg aaaggagatc agcgatgccc aagggatcga tcaccagcta tatcgatgtg
    13021 gcgcaggtta cactgtacct gttctggttt ttcttcgccg ggttgatttt ctatctcctg
    13081 cgcgaaaaca agcgtgaggg ctacccgctc gattccgatc gaggtggaaa catcaaggtg
    13141 cagggcttcc cggcgattcc cgtcgcgaag acgtacctct tgcctcatgg tggcacgcag
    13201 acagcgcctc gcgacgagcg agactcgcgc accctccagg ccaagccctc agcgccctgg
    13261 cccggagcgc cgctggtccc aaccggcgac ccgatgcgcg acggtgtcgg tccggcggct
    13321 tgggccgagc gtgcagacaa acccgatctc tcggtcgatg gccacaatcg catcgtgccg
    13381 ctgcgggtcg cgacgggctt ccatctcgag acgcgggatc cgaatccgat cggcatggca
    13441 gtggtcggcg ccgacgggct tgtcggcggg acagtagcgg atgtctgggt cgatcgctcg
    13501 gagagcctca tccgctatct cgaggtgaag cttccggccg ctgccggcgc agcgcccgcc
    13561 gatgcggcaa ggaccgtgct gctgccgatg acgttcgccc gtgttgccgg caaccggcgg
    13621 ttggtccacg tgcgctccat cctcgcctcg cagttcgcgg gtgtcccggc catcagtacg
    13681 cctgacgcgg tgacacgacg cgaggaggat cgcatctgcg gctatttcgg cggcggcaca
    13741 ctctacgcga cgcccagccg gcaggagtcg ttgctatgac ccacgacgac tttgcacacg
    13801 agccgatccg tggcctgccg gagcggccgc cagccggcga ggaggtgctc tggcaggggg
    13861 cgccgcattg gccgacgctc gcccgccgcg tcatgcacat cgatctcgtg gctgcttact
    13921 ttctggcgat tggagcctgg aaggtggcga atggcgtcgc tacaggtctg ccgggaactg
    13981 agattgccgt gtcgcttgct gccttcgctg cattgtcgat cctggcggtc ggcgtgctgg
    14041 cactgctggc ctggggcatc ggtcgcacca cggtctacac catcacaagc cggcgagttg
    14101 cgatgcggtt cggcattgca ttgcctgtca ccttcaacct gcccttcagc gtcatcaaca
    14161 gcgctggcgt ccgcgatctc ggcggcgggc gcggcgacct cgctctcgag cttggagccg
    14221 ggcagaggat cgccttcctc gtcctctggc cgcacgtccg gccgtggcgg ctcaggacgc
    14281 cagaggcgat gtttcgcgct gtgctgtatg ttgcgcgcgt ctccgggatt ctcagtacgg
    14341 cactcaaggt cgagctgcag cggaccggcg tgcggacagt tgcgtcatcg gtgcggcagg
    14401 gtcgcgagcc ggaggccgtg attagccacc agccgatcgc acgtccagcg ctgactgcgg
    14461 ccgaatagcg aagaggaggc acccatgcac gacaatgcca agccctgggg tcagccgggt
    14521 tggatgctcg ccagtgccag cctcctggtg gtggcagcga tatcgctggc ggcaatcgcg
    14581 cgctactcgg ggatcggcgc ctcacgcatt ggcgagccgc atgcggttcg cgtcatcgac
    14641 cttcggttcg aggatcaggc cgccggtcgg atcctggtaa ccgacgtctc ggggcggctg
    14701 ccgccgcgca tgatcgagcc gggaaaggac ggctttgtcc gggtcgcgct gagaagcctt
    14761 gcgcgcgacc gggcactgat cgggattgga agcgaggtgc cattccgtct cggcgagcag
    14821 gccgacggga gcatttggct gcgcgatctc gcgacgggcc gcctgcttct gctcgatgcc
    14881 tatggccatg ccaaccggac gagcttctct cagctgctag cccacgcggg agcgacacca
    14941 tgatcaggct tttgaggcca cggacccaat tcgacattcc gtgcacgatc gagatcgaga
    15001 acacgttcga gagcctgcat gcgcacgtcg agctcgacgg cgacgtcgcg gttgaggccg
    15061 gcgacgaggt gctcgttcat ggcgatacga tccgcgtgcc ctacgggcag aaaatgaccc
    15121 tccgcaggca ggcgacggtg agacgggcga gcggcattga ccggctgctg accaagctcg
    15181 cagcgcgctt cgagctgacc gagctctatg aggtgagttt ctcgtccggg aggaagctat
    15241 gaccattgaa tccctcggcg ttgcgccgaa cgaggctacg cggaccgcgc agcaggatgc
    15301 gctcctcacg ccgcgcttct acacgaccga ttttgccgag ctcgacgcga tgagcgtcga
    15361 gagcgtgcgg cgtgaatggg acgagctcct cgcggagatg cgcagcgatc cgaacaaggg
    15421 ccatttccga cgcacggaag cctgggatgc cgcggatctc gagatgctgc ccgagggcct
    15481 gcgcaaagag ttcaaggatt tcctgatccg ctcacagccg agttctcagg ttgcgtgctc
    15541 tacgccgaga tgaggaagcg cggcaagaac cctgatatcg tcgagctctt caagtacatg
    15601 agccgcgacg aggcgcgcca tgccggcttc atcaacgata cgctcaagga cagcggcatg
    15661 ggcgtggatc ttggctttct gacgaaggcc aagaagtaca cgttcttcag gcccaagttc
    15721 atcttctatg cgacgtatct ctccgagaag atcggctatg cgcgctacat caccatttat
    15781 cgccatctgg agcgccaccc cgaccggcgc ttccatccga tcttcaaatg gttcgagaag
    15841 tggtgcaatg acgagttccg ccacggcgag gcatttgcgc tgctgatgcg gtccaatccg
    15901 gcgctgctca gcgggatcaa cagactctgg atccgcttct tccttctcgc cgtctacgcg
    15961 acgatgtacg tgcgcgacca tgcccggccc gagttccaca aggcaatcgg catggatccg
    16021 aaagagttcg gctcgcgggt cttccacatc accaacgaga tctcgcgcca ggtctttccg
    16081 gtcaccatcg atatcgacag cccggccttc tgggcgtgcc tcgagcggct gcgtcgaatc
    16141 tccgatgcca tgaacgagct gcccgagggt ggtggacttg ggacgcggct gcagagagcg
    16201 gcgctcggca tccgggccgg cgcgacgttc ctcaggctct tcctgatccg gcccaagacg
    16261 aacgagctgc ctgacagcag ccgactccat ccagcctggt agacggtagg cggtgcacgt
    16321 tccatgatgc tcgatgcgag cccaattgcg attgcagcct gcctggtctg gtgggcctcg
    16381 acaggcgcga tcctgtcgct gatcggtcgc ggcgaaagca cctatcgctg gagcatgttg
    16441 ggcgccgcgc tgctcggcgt ggcggcgatc gccgcagtcg tcgcgagcag ccgcgcgacg
    16501 acgacgcagg gggcgttcat cgccttcatc gccgccatcg gtatctgggg cgcgatcgag
    16561 atgtcattcc tgatggggtt cgttgtcgga cctcgtcggg ggccatgtcc gccgggcctc
    16621 acgggctggc agcggttcaa ggtgtcgttc gccgcgctcg cccatcacga gctgctgctg
    16681 gtggctgcgc tggcagcact cgcctggctc gtcgccgatc agccgaaccg cgttgccctc
    16741 tacacgttcg cgcttctctg ggtgatgcgg ctgtcgacga agctcaacat cttcctcggc
    16801 gtctcgaatg tcagcgagga ggtacttccg gcgcgtatcg gctatctgaa gagctacttc
    16861 cgcaaggcgc cgatgaatgc gctcttcccg ctctccgtca cgggcgcgac ggcatgcgtc
    16921 ttctatttcg gaggcctggc ggcaagcccc ggtgcaagcg atttcgagcg agcatccgcg
    16981 gctctccttg cgacattctc ggcgctcgcg ctgatcgagc actggatgct cgtcctgccg
    17041 ctggcgacgg ccgcgatctg gccatgggcg ccagcgccgg agcgttccgc aggatcgctc
    17101 gcgatcggcc atccaactaa cgcagcacac gccgggagcg atccggcagc gaccggcgat
    17161 cgaccgcacg tccggccaca agtcatttca tcagcggtcg ggcggggcgc gagccctgtc
    17221 gaggcaacga tcgtcgacat ccctcgccaa ccttggagga caaaatgaac tacgaagccc
    17281 agttcgagag ctatctcaat tcgcttcact ccgagggtcg ttatcgcgtc tttgcggacc
    17341 tgatgcgcaa gtgcaacgag ttcccgcgag cggtgcgcta caaggccggc tcggaaagca
    17401 atgtgactgt ctggtgctcg aacgattatc tcggcatggg tcagagccgg atcgtgctgg
    17461 acgccatgca cgaggcgctc gatcgctgcg gtgccggttc cggcggcacg cgcaacattt
    17521 caggcacgaa ccactaccat atcgagctcg aggctgaact cgccgacctc catcgcaagg
    17581 aagcggcgct cgtatttacg tctgcctacg tcgcgaacga cgctgcaatc tcgagccttg
    17641 tcaagctgct gcccggcgcg gtgatcctct cggacgagaa gaaccacgcc tccatgatcg
    17701 caggcatccg ccacggcggc ggggagaagc acatattcaa gcacaacaac ctcgtcagcc
    17761 tggagagtca gctgaaggcg ctgcctcgcg acgtgccgaa aatcatcgcc ttcgagagcg
    17821 tctactccat ggacggctac atctcgccga tcggcgcgat ctgcgcgctt gcaaagaagt
    17881 atggcgccct gacctacctc gacgaggtgc atgccgtcgg tatgtacggg ccgcgcggcg
    17941 gcggcatcgc cgagcgcgac ggtgttctcg atcaggtcga catcatcaac ggcacgctcg
    18001 cgaagggctt cggcgtcatg ggcggctata tcgccgcgag ccgaagctgc tgcgatgcca
    18061 tccgctcatt cgcgccgggc ttcatcttca cgacctctct tgctccagcg ctggccgccg
    18121 gtgccctggc gagcatccgg cacctcaagg tcagcatgct cgagcggagg cgccatcagg
    18181 agcgcgcggg ctatgtaaag cgcgcactcg gcgaagcagg gctgcccgta ctggagaacc
    18241 cgagccatat cgtgccgctc atggtgtgca accccgtgca ctgcaagttg atcagcgacg
    18301 cgctgctgga cgcccacggg gtctacgtcc agccgatcaa ctatccgaca gttccacgtg
    18361 gcacggagcg gcttcgcatc acgccaacgc cgctgcacag cgatgccgat gtcgatcatc
    18421 tcgtcgcgtc gctgaaagcc ctctgggaga catgcccgat gtcgagcact gcgagcctgc
    18481 agcatgcagc ggagtaggag gcgccgaggc gcttacctgt tgccagagtg accgatccga
    18541 ggagggatgg agcatgagcg acttcagtac gacgccgctg ctcgacaagg ttcaatttcc
    18601 cgaggacctg cgtgcccttc cggatcgcga gctccgccag gtcgccgatg agctgcgcaa
    18661 ggagaccatc gcagccgtct cgatcacagg cggacatctc ggcgccgggc tcggtgtcgt
    18721 cgagctgacg gttgcgctgc atcacgtctt cgatacgccg tcggatcgga tcatctggga
    18781 cgtggggcac caggcctacc cgcacaagat actcaccggg cgacgcgacc gcatccggac
    18841 cctccgtcag ggcggcggcc tttccggctt cacgaagcgt tccgagagcg agtacgaccc
    18901 cttcggagcg gctcattcct cgacctccat ctcggcgggt ctcggcatgg ccgtggcgcg
    18961 ggatctcacc ggcggcacca acaatgtcgt ctgtgtgatc ggtgacgggg cgctcagcgc
    19021 cggcatggcc tacgaggcga tgaacaacgc cggcgctcgc gatgcgcggc tcatcgtcat
    19081 cctcaacgac aacgacatgt cgatcgcccg gccggtcgga gcgctgtcga gctacctcgc
    19141 acggctcacg acgagcggcg cctacctcca gttccgcgac gcgctgaagc agctcgcgcg
    19201 gcgactgccg aaagcctggg agcgtcgtgc agcccgcgcc gaggagtata cgcgcaccta
    19261 ttggacgggc ggttcgctgt tcgaggaact cggcttctac tacgtcggcc cgatcgatgg
    19321 ccatgacctc gaccagctgc tcccggtgct gcgcaatgca cgccaggcga aacacgggcc
    19381 gatcctgatc catgtcgtca cgaagaaggg gaagggctac gccccggccg agacggcatc
    19441 cgacaagtat cacggcgtca atcgcttcaa cgtggtaacg ggcgagcagg tcaagacgcc
    19501 ggccaatgcg ccgagctaca cgcgggtctt cgcgcagggc ctgatccgcg aggccgagtt
    19561 cgatccgcgc atcgtggcgg tgacggccgc catgccgagc ggcaccggtc tcgatctctt
    19621 cgggaggcgc ttccccgaca ggaccttcga cgtcgggatc gccgagcagc acgccgtgac
    19681 gttcgcggct ggcctggcga ccgagggcat gaagccgttc gtcgcaatct attcgacctt
    19741 cctgcagcgc ggctacgatc agatcgtgca cgacgtggcg ctgcagcgcc ttccggttcg
    19801 ctttgccatc gaccgcgccg gtcttgtcgg ggcggatgga gccacgcatg cgggctcctt
    19861 cgatgtcgcc tatctcggaa acctgccagg catggtgctg atggctgctg ctgacgaggc
    19921 cgagctcatg cacatggtcg cgaccgcagc tcgcgtcgac gaccggcctt cagcattccg
    19981 ctatcctcgc ggtgacggtg tcgggctcga actgcccgag cgcgggacgc cgctcgagat
    20041 cggccggggc cggatcgtgc gcgagggaag cgcggtggca cttctctcct atggaacgcg
    20101 actctccgag tgcatgaccg ctgcgggcca gctcgccgcc atgggtctat ccgcgacagt
    20161 tgccgatgcg cgcttcgcga agccgcttga caaggccctc gtcgaccaac tcgtccgcca
    20221 tcacgaggtg ctgatcacgg tggaggaagg ctcggtcggc ggcttcgggt cgtttgtgct
    20281 ccagcatctc gcggcctccg ggcggctcga ccgcgggctg aaggtgcgta cgctggtcct
    20341 gccggacgag ttcatcgacc acgacaagcc agaggtcatg taccgcaagg ccggcctcga
    20401 tgcggcgagc atcgtcggtg ccgtctgcca cgctctcgag atcgataggc cggcaagggc
    20461 gtcgcggctg caatctgggc aatgaggccg acggccatga cgagcgcagg ggagagtccc
    20521 atcgcctcac cggatctgga gatccgacgg cagaaaagtg gcggcgagcg gcgcactgca
    20581 ctcggcgagc acgacggtcc ttcggatatt gcgcgccgga agtccgagca tgtcgaagtt
    20641 gtcctgcacg gcaatgtcgg ggcgcgcaat gccacgaccg ggttagagaa ggtccggttc
    20701 gagcactgcg cgcttcctga gctctccctc gacgacatcg atctctcgac gcgccttgcc
    20761 ggccgaccct tggcggcgcc gctgatcgtc agctccatga ccggcgggcc gctgcacgcc
    20821 cggcgcatca actatgcgat tgccgaggcg gcgggccggg ccggcatcgg cttcgccgtc
    20881 gggtcacagc ggatcgctct cgaaggccgg gcgagtgctg ggttcagtcg gaagctgcgg
    20941 tcgctagccg gcaatgtccc gatcctcgcc aacttcggcg gggcgcagct acgtcactgg
    21001 tcgggcacgc tcgaggccga gcgcgccatc gagatgatcg acgcggacgc gctcatcatc
    21061 catctcaatc cgctccagga ggccgtacag cacggcggag accgcgactg gaccgggctc
    21121 ttgcgccgga tcgaggagct tgcgcgcgat acgtcgcggc cgttgatcgt gaaggaggtc
    21181 ggcttcggga tttccggtcg cgtggcgcga caactctgga atgcaggtgt cacgatcatc
    21241 gatgtggcgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ggcgtgccca cggcaagggc
    21361 gatcaccgac gttcgcgagg cgtgtccgca ggtgaccatc attgcctccg gcggcattcg
    21421 cgatggtctc gatgtcgcgc gagccatcag gctcggcgcg gacttcgctg gccaggccgc
    21481 aggcatcctg cccgcggccc tcaacggtgc cgacgctgtt gcagagcaca tcgcaattct
    21541 cgtcgagcag ctacgcatcg cctgcttctg cactgcgagc gccgaccttg ccgcgttgcg
    21601 ccgggcgccg ctcctctccg gggcggattg aggggatggt atcgtggcgc aggaccgtgg
    21661 tcatggacgc ccgctggcgg cgacagcgga ttttgtcgcg agccgagcag cagatgccgg
    21721 cagtcttcag gtgatgccga gcgcgaggcc tgctgatgcg gggtcatgcg gtgatggggc
    21781 cgaaccgctt ccgacggcga tcgtcgtcgg cgggggcatc ggcggcatgg cagcggccct
    21841 gcgactgaga tcgaagggct gggcggtgac gctcatcgag cggcaggcgg agctcggtgg
    21901 acgcgcgcag gtctacatcc atgacggctt tcgtcacgac gccggcccga cgatcatcac
    21961 ggcgccgttt ctgatcgacg agcttttcga gctgttcgga cggcggcgcg aggactacct
    22021 gaggctggtc ccgcttgcgc cgtggtacag gttccacttc cacgacggca ccacattcga
    22081 ctacggcggg accatcgagg acactctcgc cgagatccgg cggatcgagc ccagagatgt
    22141 tgaaggctat cggtgcttgc tcgagcattc gcgctccctt tacgacattg ccttcacgaa
    22201 gctcgcgacg cggccgttcg actcgctcag catgctcctc gcccagacgc cgcggctcgc
    22261 cagactgcgc gccgatcggt cggtctggaa gctcatcgcg cgacatctgt cgaacgacaa
    22321 gctccgccgc gcattttcga tccagcccct tctcgtcggc ggcaatccgt tcacgacgac
    22381 gagcatctac gcgctgatcc actatctcga gcggcgctct ggtgtcttct tcgcgatggg
    22441 tggcacgggc gcgctggtta ccgcgttgcg tcgtcttctc gcggagaccg gcgtgatcgt
    22501 gcgcactgcg acgaccgtcg agaaggtgct ggtcgagcgg ggcgaggcgc gcggcgtgcg
    22561 gctcgcagac ggtgaggtga tccggtcccg ggtcgtcgtt tccaatgccg acccgatgca
    22621 tctctatggc cacatgatcg accgtgagca catccgcctc acaacgcgag caaagctcgc
    22681 gtcgcggctc agcatggggc tcttcattct gtatttcgga acgcggcgga cctacccagc
    22741 tgtggcccac aacaccatct ggctcggcga gcgctacaag gagctgctcg ctgatgtttt
    22801 caggaacggt gttctggccg acgacttttc gctctacctg caccggccga ccgctaccga
    22861 tccgagcttt gcgccgccgg gctgcgacag cttctatgcg ctgtgtccgg ttccgaacaa
    22921 tgctgcgggg atcagttggc gcgtcgaggg tccaaagctc cgatcccgcg tcattgcagc
    22981 gctcgaccgc acgatacttc cgggtctcag cgaatcgatc acggcggact tcttcgtgac
    23041 gcccgacgat ttcgccgacc gcttcttggc agtcgaaggc agcggcttct cggttgcgcc
    23101 atatttcctg cagtcggcct ggttcaggtt ccacaaccgg tcggaaagca tccgcaatct
    23161 gcatctcgtc ggggccggga cgcatcccgg cgccggcatt ccgggtgtcc tctgctcggc
    23221 gcggattgcg gcggatcaga ttggtgaagc ggctgttggg tccgtcgccg tcagtgaatt
    23281 ggcggcaggt atccggtagc tccgggcggt cgacagacaa gagggcacct gcgagatgcg
    23341 cggagcatgt aaattttatt ggacaaatta aaacgtacaa tacaagttac gttatgtcga
    23401 aacccagatg agccggaggc acggccgatc atggatccac gagagagagt tgagctggca
    23461 ctcgaaagct ccgtaatggg agcgtctcgc gacggttgcc cgccgggtct cgctgcagcc
    23521 gtccgccatg ccatcttccc tggcggcgcc cgtgtacggc cactgctgtg tctcggcgtg
    23581 tctgtcgcgt gtggtgacgg cgcgcccaag gcggccgatg cggctgccgc ggcaatagag
    23641 ctgctgcatt gcgcctcgct cgtccatgac gacctgcctt gcttcgacga tgccgatctt
    23701 cggcgcggca aaccgtccgt gcatcgtgcc ttcggtgagc cgatcgcggt gctcacgggc
    23761 gacgcgctga tcgtgctcgc tttcgagacg atggccatgg cctgccgcaa tgctccgcgc
    23821 atgctgccgg acctcgtctc gatcctgtcg aaggccgttg gcatgtcggg cgggcttgcg
    23881 gccgggcagg cgtgggagag cgaggcgaac gtgcccctcg cgacgtacca tggagccaag
    23941 acgggctcgc tgttcgttgc cgcaacgaag gcaggcgcgc tcgcggcagg ggcggatcct
    24001 ctcgcttggg aggagttcgg gtcgaggctc ggcgaagcgt atcagattgc cgacgatatt
    24061 cgcgatgcct gctcgacaag tgcggagctc ggcaagcccg taggtcgcga cgtggcgctc
    24121 gggcggccga gtgcgatccg gaatttcggc ctcgaggatg ccttcaagca tctcgacggg
    24181 ctcgtggaca gcgccttgtc agctgtgccg gcctgcccgg gtgccgcgga gttgaaaagt
    24241 atcgtgcgcc gccaggtgcg ctggttcgtg ccgaaagaga gtgcgctcca cgccgcataa
    24301 ggccggcgat cggggagcag gctgccgggg aaactcaaga tgctacgcga gccaaggtcc
    24361 gacaagtcgg cgccacatat gcagcccgtc accccgtccg cggctgctgg gtcaaaggag
    24421 cggcaggccg atcttgcgtc ttctggagcc gcttttgcac gggggttccc gttctctgca
    24481 atcgtcgggc agaccgagat gtgcacggcg ctcctgattg ccgccgtgga gccgaccatc
    24541 ggcggtgttc tcgttctcgg cgatcggggc accggaaaat caaccgctgt cagggccctg
    24601 tcggcgctgt tgccgcctat cgaggtgacg gtcggttgcc cgttcggctg caatccgacc
    24661 taaggcgtgc gctgcacggc atgtgtcatt gccgcagcag gggagccgct gcggtcgaga
    24721 cttgcacccg tgccgatggt ggatctgccg cttggctcga cgcaggaccg ggtgctcggc
    24781 tcgctcgacc tcgagcgagc cctcgcagag ggggtcaaag ccttccaacc ggggctgctc
    24841 gcggcggcta accgcgggtt tctctacatc gacgaggtca acctgctgga tgatcatctc
    24901 gtcgatctgc tgatcgatgt ggcggcgtcg ggcgtcaaca tcttcgagcg ggacggccta
    24961 agcgtgcgcc accccgcccg cttcgtcctg gtcggcagcg gaaatcccga ggaaggcgag
    25021 ctccgccctc agctcctgga ccgcttcggg ctctgcacgg aagtgcgcac gccgaccgac
    25081 atcgcctcgc gcgtccagat cgtcaagcgc agggatgcct atgagcgcga tcaggaggcg
    25141 ttctgcgagt cgtggcgcga cgaggacacg cgcattcggc gcaagatcgt cggggcccgt
    25201 gccggcatcg gtcgagtgac cgtctccgat gcggtgctcg agcaaatagc tggcctgtgc
    25261 ctgtcgcttg gcacagatgg aattcgcggt gagttgacgc tcgtccgggc ggcgcgcgcg
    25321 cttgccgcgc tggatggcct cgaaacgatc gaggcatcgc atatcaagac agcagcgccg
    25381 ctgacgctgc gtcacaggct gcgccgcaat ccgctcgacg agaccggctc atcggctcga
    25441 atttcgcgag ccctcgacga gctcgatctc gaatgacggc tttgcgcgag agcggcgacc
    25501 gcgcccgacg ggcgagcgac gccgcactcg ccgcgatcct cctggcagtg gcccctcgcg
    25561 agctcggtgg cgcatcgata cggttggccg ctggatcggt cgccgaggca tggatcgacc
    25621 ttgctgtgcc ggaggtcccc gggaggccga ggcctgtccg actgccgatc ggtctcacca
    25681 acgagcagct tgccggcggt gtcgatcttg cagcgacgct tgcggtcggc cgcaaggtca
    25741 tgcagccggg gctcatcgag cggaataagg gctgcgccct gatcgtcccg tccgccgagc
    25801 gctggtccgc cgaggcagcg gcgtgtctgg tcgggcgtat cgaccagcgg tgcgacgccg
    25861 atgcggacgg gccgactgaa ggccatctgc tgctcgcact tgacgaaggg tgtggcgagg
    25921 acgagcgacc gccggagctg ctctgcgatc gtctcgcgtt tctcatcgac ctagccggac
    25981 ttcgcgaggc cgacgtcgcc ggccctacgg acctcacgga tcgtctggag gcggcgcgag
    26041 cgcttcatcc gaatgtcgcc gtt
//
LOCUS       DAORNH010000023        25317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1506_length_25317_cov_2.931359, whole genome shotgun sequence.
ACCESSION   DAORNH010000023 DAORNH010000000
VERSION     DAORNH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 25317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25317
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_25317_cov_2.931359"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PK264_04180"
     CDS             complement(<1..1156)
                     /locus_tag="PK264_04180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRD75124.1"
                     /translation="MKLTLSWLRDHLDTDATAEALAAKLSMIGLEVESIENPAKALAP
                     FIVARVLDAKKHPDADKLRVAQVDTGAGVVEVVCGAPNCRTGMIGVFAPIGTYIPGTK
                     ITLDKRPVRGVVSNGMLVSERELELSDDHQGIIDLGETFATSIGKRYIDALGLDDPVL
                     DVKLTANRPDCTGIRGIARDLAAAGMGKLKAEPKLAKVEGSYDCPVPIDVDGADASGC
                     SAFYGRYIRGIKNGASPAWMQARLKAIGQRPINAVVDVTNYVSFDRGRPLHVYDADKL
                     HGTIRARIANPGEGFKGLDGKSYVVEPPMCVIADDRAVLGLGGILGGEASGSTNETKN
                     ILIECAYFDPLKTAATGRRLGLQTEARYRFERGVDPAYLDAGLDLATHLMM"
     gene            complement(1225..>1762)
                     /locus_tag="PK264_04185"
     CDS             complement(1225..>1762)
                     /locus_tag="PK264_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRD75125.1"
                     /translation="RTMMSQKPPIRIIAPGRVYRLDSDRTHTPMFHQIEALVIDEATH
                     MGHLKWCVEEFCRAFFEVDSVNMRFRASHFPFTEPSAEVDIQCDRSGGQIKFGQGSDW
                     LEILGSGMVHPNVIANCGLDPEKYQGFAFGMGLDRIAMLKYGIPDLRDMFAADLRWMR
                     HYGFLPLDVPTLAGGVSS"
     assembly_gap    1763..1846
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1847..2331)
                     /locus_tag="PK264_04190"
     CDS             complement(<1847..2331)
                     /locus_tag="PK264_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRD75126.1"
                     /translation="MSSTIDPAPIEAQLLADVAKAADLASLEAVRVQALGKKGQISEL
                     LASLGTMKPEERRTFGQTVNAVKVRVTEAIEARKGELGRAEVTARLARERADVTLPVR
                     LGGMADGRIHPISQVYDEIIEIFADMGFSVAEGPDIETDDLNFTKLNIPPEHPARQEH
                     DT"
     gene            complement(2485..2856)
                     /gene="rplT"
                     /locus_tag="PK264_04195"
     CDS             complement(2485..2856)
                     /gene="rplT"
                     /locus_tag="PK264_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRD75127.1"
                     /translation="MARVKRGVTAHAKHKKVLKAAKGYYGRRKNTIRVAKQAVEKALQ
                     YGYRDRKRKKRTFRALWIQRINAAAREHGMTYGRFIHGLEQAGIAVDRKVLADIAVKD
                     PGAFKALVGQAQAAMPATAKA"
     gene            complement(2911..3111)
                     /gene="rpmI"
                     /locus_tag="PK264_04200"
     CDS             complement(2911..3111)
                     /gene="rpmI"
                     /locus_tag="PK264_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRD75128.1"
                     /translation="MPKLKTKSSAKKRFKMTGTGKVKAQAQGKRHGMIKRTAKFIRTA
                     RGTMVLNDSDAKIVKKYMPYDR"
     gene            complement(3330..4337)
                     /locus_tag="PK264_04205"
     CDS             complement(3330..4337)
                     /locus_tag="PK264_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75129.1"
                     /translation="MKARIVGALAALALAGTAATSAVRAEVKEVILGQQFGAVYLPMV
                     LMEHLKLVEKHLEAKKMGDVKVKWAKLGGPAALNDAFLSGSLHFAAQGVPSMALIWDR
                     TKGEIKAVAAIANNDIWLNTRNPNIKSLKDFTEKDRIAIPSLRVSTQALALQIAAEKV
                     FGVGGHNKLDHIIVPLPHPEALAAVLNPVHEINTHFATSPFNEVELKSPNIRTVTTFF
                     EIMGGPSTGLTWTSTSKFRSENPTVYAAVNAAYDEALALISKDKKAAGRLYLVAANDR
                     RTSPETMDELMMGKGLEFTKVPSNVGKFVEFMHRVGTIKTKPTSWKDLFFPEIHSANG
                     T"
     gene            4490..4795
                     /locus_tag="PK264_04210"
     CDS             4490..4795
                     /locus_tag="PK264_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD75130.1"
                     /translation="MTRPARPAAKPTQFVDNERAIVTEWRFAPGAETGWHRHGHDYVV
                     VPMTNGELLLEEPGGTSRTAPLTAGVPYFKSEGVEHNVVNASSHEVVFVEIEFKRKA"
     gene            4924..5553
                     /locus_tag="PK264_04215"
     CDS             4924..5553
                     /locus_tag="PK264_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HRD75131.1"
                     /translation="MVESQVRPSDVTDRRVLRAMAQVPRERFVTSGLRSIAYSDGPVK
                     VSDQPVRYLLAPRTLGKLVQLLEIGDRDVVLVLGCSTGYSTAIIARVAQSVVAVEEEA
                     ALADKAQTLLADLGINNTAVVAGSLTSGKASEGPFDAIFINGSVEEVPAAILDQLKDG
                     GRLVTVEPHDGVGRAVQWRRSGKTYGRRVAFDAAAPRLSEFDKKREFVF"
     gene            5607..6569
                     /locus_tag="PK264_04220"
     CDS             5607..6569
                     /locus_tag="PK264_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75132.1"
                     /translation="MLAVSGIIAAAPAGASPPDKRSPIPAGLPVAVDVMGASEPVLCA
                     EKDNVTVNFTAPGVRAFRIEAAHPAYIGSLVADSFAADWAACDMTGDPAHAGAVTAPT
                     RRTIYEEPEIWLVGWTFPTFWRPATATVRIGDRVERGLHLVQVWMIRPMGGEEILVFY
                     PQDGYWRARPLAPAGKAPTAFGSSFLLGPVEVDRRPIVRLDEIAFDPKARSFTLKFAR
                     GGSATLSLAAIDQNRTILDIAFDRAIEGVPFAALRSMYVTETNNDVARIGIRAKGAKG
                     WSEEPIMSFKRAAATSVWAGRVAPSRHNTSSPDMIFDSFRGAPR"
     gene            complement(6573..7949)
                     /locus_tag="PK264_04225"
     CDS             complement(6573..7949)
                     /locus_tag="PK264_04225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD75133.1"
                     /translation="MSVATAKFVICGAGIAGVAAVYQLAVVHGCRNVVLVEEGNPLSL
                     TSDKSTEAYRNWWPGPDQAMTAFMNRSIDLIEGLAQTTGNRFNLNRRGYVFASADPAK
                     ADWLIENARVAEARGAGAVRVHGAGSRSYVSAAAHGFESPLTGADVITDRALIREYFP
                     YLAPETRCIAHVRRAGWLSAQQLGMTMLDAAREHGVRTLRGRVTGAETQGGRLVSVNI
                     ESGEGRANLEATHLVLAAGPKISPLAASLGIELPLFAERHLKISIQDTLGAVPRHAPM
                     LIWLDPQRLPWNGEEREALAADDSMRWLTEPFPAGVHGRPDGGPQSATLLILYNYHVE
                     PSDVVYPLAEDENYGEIALRGMSTIVPALGAYLDKGVRPYIDGGYYMKTRENRPLIGP
                     SSVDGIYLSCGYSGFGIMSSMAGGELVARHMLGLAPPDYAPAFLPTRYSDPAYQALLA
                     NWGDGAQL"
     gene            complement(7996..10263)
                     /locus_tag="PK264_04230"
     CDS             complement(7996..10263)
                     /locus_tag="PK264_04230"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HRD75134.1"
                     /translation="MPYSDYRPELMGRATLEPTGQFEAGSMQSFTLVYTAGKFGIDDT
                     GSIKIGFRFATDFGPVQFDKPEAAGYTTAEASNGATLELKWEFKRNIRPWSRSLYVGV
                     MKHFLKPGDTITIRFGDRRQGSPGIRLQTYAERAFEFHVLVDAIATYDYVALPESPKI
                     AIVAGSGVQWRAVLPTLVRSGEAFRFGVRADDKWGNPSPHVPRSLIVEPSRPVAGLPR
                     TIEFAPGAQSAIVDGLTLDDDGELQIAVKTTDGRELVRSNTLHVAPTDASLVHYWGDT
                     HGQSDETLGTGSAREYFEFGRDRAFLDVMGHQGNDFQITGAFWRDLNELTRSLDKPGR
                     FVAIPGYEWSANTAVGGDRNVHFRHEGETIHRSSHAQIADVGDMVDEHTDAHTAADLF
                     RALAGRDCVVAAHVGGRYADITLAHDGRLETAVEVHSAWGTFEWLLHDAFAKGYRVGI
                     VANSDGHKGRPGACTPGASFFGSMGGLTCFLAPRLDRDAVFEAMRRRHHYATTGNRMS
                     LAVEVTTARPAVLFERDPDHFPDTRIADTRHMIMGDIAEVADAEVDLVIDVVGSAPIE
                     RVDVFDATKLIETYRPFSDSDLGARIRLLYQGAEYRGRARTTIWDGVLELEANGIASA
                     SVLNNWNLDRGIQKQSRTSLEWKAVTTGNFGGIDIRLEAAREGRLAFETAHCQGILVL
                     AQIGLEDRTFEGGGLDRAVRFYRLPDRMSERRVTLRRKIRLSDTGDTRLYVRVTQEDG
                     HRAWSSPIYLYRSDV"
     gene            complement(10455..>11249)
                     /locus_tag="PK264_04235"
     CDS             complement(10455..>11249)
                     /locus_tag="PK264_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD75135.1"
                     /translation="KNKGLQFFSAVPFGLTGNETSAWMEFGGGQQIWDKILDKFGLKG
                     FHGGSTGVQMAGWFNKEITSVDSVKGMKMRIPGLGGEALKKLGMTTVNLPGGEIFAAL
                     QSGAIDATEWVGPYNDLAFGFYKVAKFYYWPGMHEPGTTLEITVDKKKFTALPADVQR
                     IFTECARAESRLLTAEFDARNGQALETLIKTHNVTLKRLPNDFLAAYGAASGQVIQEL
                     LDDKDELTKETAASYIKARRELMAWNRIAEQGYMNARLLDYKFPGQ"
     assembly_gap    11250..11273
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11274..11598)
                     /locus_tag="PK264_04240"
     CDS             complement(<11274..11598)
                     /locus_tag="PK264_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HRD75136.1"
                     /translation="MKSEKRETRVRRRQFLAGAAVVGAGAGTSAIVTPAIAQAKTEWK
                     MVTTWPKNFPGLGTGANRFAESVTAATDGRLTIKVFAAGELVPPFESFDAITRGAADL
                     MHAAPY"
     gene            11879..12199
                     /locus_tag="PK264_04245"
     CDS             11879..12199
                     /locus_tag="PK264_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75137.1"
                     /translation="MTFPALAAAAAVLTLGIPAINASAQDAPKKAKAAAKAAPVKANC
                     TTRYGKGYAWTESMARFQAWEIIAQTTGNWPIMMDKFRNEVYKCKPDGSGFTCHSKID
                     VCKS"
     gene            12272..13102
                     /locus_tag="PK264_04250"
     CDS             12272..13102
                     /locus_tag="PK264_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75138.1"
                     /translation="MLLEDKVAIVTGAAQGIGKACAMRLAAEGAKVVIADVNEEQGAA
                     LVATLKESSHDAIFVSCDVAERLEVHNLVAATLDAFGRVDALVNNAAVIDDVAFLELD
                     EAEFDRVLDVNLKGAFLVGQACAKQMVKQAAKQSRGSAGAIVNMSSVNENFALPDHVA
                     YTISKGGIAQLTKSMALALAPYRIRVNAVGPGTIETPITASVIRDEAMRAKALSRTPL
                     GRFGRPEEVAAVVAFLLSDEASYVTGTTVFVDGGRLPLNFVMPTADKSRTEPADDEDG
                     "
     gene            complement(13129..13887)
                     /locus_tag="PK264_04255"
     CDS             complement(13129..13887)
                     /locus_tag="PK264_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD75139.1"
                     /translation="MTSVTDPVPKFELVLYHAWASSASRKVRFALEEKGLAWTGVVVK
                     LLENEQLTPDYLKLNPNGVVPTLVHHGRVVIESSVINEYLEDVFPDRALRPVDPLTRA
                     HMRVWTQYVDTDVIKLFMVLNWNRMMGPTASRWSESQLGDKLARTPMPERAEAWRRVA
                     REPFTEAERQRAVAGIRHCLDKMERDLGDGPWLAGDMFTLADIALSPYIVRIGETAEL
                     GLTLEGYPRVRDWWQRLTARPAFARARIEALKLG"
     gene            14097..14618
                     /locus_tag="PK264_04260"
     CDS             14097..14618
                     /locus_tag="PK264_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HRD75140.1"
                     /translation="MRYAFILLFMAVPLAEIALLVKFGQVFGFWATIGLVVGTAILGG
                     AVLHSQGLRALTRIQASAEKGEAPIGPVIDAGFIALAGFLLVTPGVITDSIGLLLLIP
                     PIRRRIALVVVRWALRNSEIRVETFRRERARTPPPGDEMSDGPVIDVKAERLDERPMK
                     PTGSDNGSGRPNH"
     gene            14693..15235
                     /gene="secB"
                     /locus_tag="PK264_04265"
     CDS             14693..15235
                     /gene="secB"
                     /locus_tag="PK264_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HRD75141.1"
                     /translation="MTDTPNTKPTAQATPPAGAKTDGQPANVTARAVAQYIRDLSFEN
                     PSVEKQIASQPEAPQMKIELNVSGKPMGKDLYESSIELKATASGKVGTIYILELTYAG
                     LFKLENIPPQALEPILLVNCPMLIFPFARRVVADLTREGGYPPLLLDPVDFAQLYFAK
                     QKQVAAAKAGGGTPPVAGRA"
     gene            complement(15242..15946)
                     /gene="dnaQ"
                     /locus_tag="PK264_04270"
     CDS             complement(15242..15946)
                     /gene="dnaQ"
                     /locus_tag="PK264_04270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HRD75142.1"
                     /translation="MTAREIVLDTETTGIDPRAGHRLIEIGCVELMGRLPTGREFHAF
                     INPERDVPADAVAVHGITGAFLADKPKFAAVVDEFLAFIADAPLVIHNATFDVGFINS
                     ELERVKRKPLQMTRVVDTLALARRRNPGGPNSLDALCKRFGIDNSKRTKHGALVDSLL
                     LAEVYMELLGERQTTLALVGGPARGTSGRGQQKSGGAKARTRPLAPRLTAEAEAEHAK
                     FVETLGPNALWKKRDS"
     gene            complement(15967..16581)
                     /gene="coaE"
                     /locus_tag="PK264_04275"
     CDS             complement(15967..16581)
                     /gene="coaE"
                     /locus_tag="PK264_04275"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420274.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRD75143.1"
                     /translation="MLLIGLTGSIGMGKSTAAGRFRHHGLLVVDADAEVHTLYEGAAV
                     PLIEAAFPGTTGNGRVDRQRLSAKLLADSSGFKKLNAIVHPLVLEAERAQLRAAAARG
                     DQLAVIEVPLLFETGGAERVDVTVVVSAPAEAQRARVLARPGMTPEKLDAILARQMPD
                     AEKRRRADYVVDTGGSIPESEAAVDKIVAELRGRKGDAYERHWA"
     gene            complement(16605..17228)
                     /locus_tag="PK264_04280"
     CDS             complement(16605..17228)
                     /locus_tag="PK264_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRD75144.1"
                     /translation="MAEARSKSSTAPRRLILASASAARRTMLDTAGVDFEVIPANIDE
                     RAIEQANDASPPSTVAAILAEAKAIEVSVRHPNAVVIGSDQVLAFDGAILHKAPDLQG
                     ARLALGRLAGRTHELHSAAVIASGGRPVWRYVATARLAMHRLSTDEIDAYLGRVGSRV
                     LSSVGCYELEGFGVRLFEAIEGDYFTVLGMPLLPLLGALRERGMLDI"
     gene            complement(17221..18075)
                     /locus_tag="PK264_04285"
     CDS             complement(17221..18075)
                     /locus_tag="PK264_04285"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HRD75145.1"
                     /translation="MTQRPASYFHLHLVSDSTGETLTMIAKAAAAQYAQVRPIEHMHP
                     LIRSQRQLDRVLQEVASTPGIVLYTMVNSELSASLEARCKELNVPSLHALEPIMRVFE
                     SYLGAAKTPIVGGQHVLDAGYFKRIEALNFTMAHDDGKLPADISTADIVIVGISRTSK
                     TPTSIYLAQRGYKTTNVPLVPSIALPEQLTRPHNTFVVGLVASVDRIAEIRRNRVRLL
                     SDRDIETYADKEQIAAEIAYSKRLYGKYGWPMIDVTRRSIEETAATIIKMYNDRHTRA
                     IDAETDDG"
     gene            18512..19561
                     /gene="hemE"
                     /locus_tag="PK264_04290"
     CDS             18512..19561
                     /gene="hemE"
                     /locus_tag="PK264_04290"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HRD75146.1"
                     /translation="MSASKPTRHFLKPFQGRATAPPPIWLMRQAGRYLPEYRATREKA
                     GGFLKLVYSPELAAEVTLQPIRRYGLDAAILFSDILVVPDALGQGVRFVEGEGPRLDP
                     IREPADIARLRPKGAGSKFEIVAATVARLRQDLPRETALIGFCGAPWTVATYMIAGEG
                     STDQADARGFAYRDPNAMQALMDILVAASIEYLSLQVKAGADALQIFDTWAGSLAEDQ
                     YERWVISPTLAITTAIRARHPGVPIIGFPRGAHASASTYLQFAGVDGISCDTSMPLSA
                     MSRLAEGGKVVQGNLDPLLLVAGGEALERRAREVVETMAGLPFVFNLGHGIVPQTPPE
                     NVARLVEVVRGAGAQ"
     gene            19558..19992
                     /gene="hemJ"
                     /locus_tag="PK264_04295"
     CDS             19558..19992
                     /gene="hemJ"
                     /locus_tag="PK264_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HRD75147.1"
                     /translation="MTDLLVKSLHVVAVISWMAGLLYLPRLFVYHCGARPGSEQSETF
                     KVMERRLYKAIMNPAMMVVWLTGPWLAWRLGHLDERWFWAKMLMVYALTWYHHVLLGH
                     LRTFAADRNTREARHFRIINEIPTLLMIVIVVLVIVRPSFRF"
     gene            20267..21592
                     /gene="rho"
                     /locus_tag="PK264_04300"
     CDS             20267..21592
                     /gene="rho"
                     /locus_tag="PK264_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRD75148.1"
                     /translation="MDNDITAAAPDAPEAPAVKEMKLQDLKSKTPTELLAFAEEVGVE
                     NASAMRKQELMFAILKQLAEKGIEIIGTGVVEVLSDGFGFLRSPDANYLPGPDDIYVS
                     PSQIRRFALRTGDTVEGHIRGPKDGERYFALLKVNTINFDEPEKQKHKVHFDNLTPLY
                     PTRALKLEIEDPTIKDLSARVIDIVAPLGKGQRALIVAPPRTGKTVMLQNIAKSITAN
                     HPEVFLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPPSRHVQVSEMVIEKAKRLVEH
                     KRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDSNALQRPKRFFGAARNIEEGGSLT
                     IIATALVETGSRMDEVIFEEFKGTGNSEIILDRKVSDKRVFPAIDVARSGTRKEDLLV
                     PQSQLRKVYVLRRILNPMGTMDAIEFLVDKLQKSKGNGDFFQSMNTPNS"
     gene            21737..>22145
                     /locus_tag="PK264_04305"
     CDS             21737..>22145
                     /locus_tag="PK264_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRD75149.1"
                     /translation="MRLGSLVRSLRLRVPGLDLGRRSRLLAASAVALTFSVVAHTGEG
                     FGQTLRQSLANAYKNNPRLDAERARLRATDEEVARANSGWRPNVTGSADVGKQTVHVV
                     PKSTADGNSSPWGYSVSVTQPVFSGFRTVNAVRE"
     assembly_gap    22146..22249
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22250..>22620
                     /locus_tag="PK264_04310"
     CDS             <22250..>22620
                     /locus_tag="PK264_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="channel protein TolC"
                     /protein_id="HRD75150.1"
                     /translation="LVKLRENHVSVLSRDLAATEARRNAREVTRTDVAQAMARRAKAV
                     SALDLARANLKSSRAAFEKATGTAPGGLVEPQPLFKLLPRSLEDALRIAEAESPNVVS
                     ALYRETAARYAVERIWGELLP"
     assembly_gap    22621..22630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22631..23242
                     /locus_tag="PK264_04315"
     CDS             <22631..23242
                     /locus_tag="PK264_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRD75151.1"
                     /translation="VRLEASYQHRLNPGASVDEQDVASVTGRVNIPLYAGGETHARIR
                     AAKHTHVSRIQEIEQAKNEVMALVTQAWSRFQAARGQLKSDKAQVEASRIALEGTREE
                     EKVGQRTILDVLNAEQELLDAEVQLATTRRDVVVAAFQLLAVMGKLSATDLHLSDTVY
                     DPDAHYAEVRRKWFGIDITHADGRLEAVDATDVHGPSWDDDGE"
     gene            23473..24927
                     /locus_tag="PK264_04320"
     CDS             23473..24927
                     /locus_tag="PK264_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HRD75152.1"
                     /translation="MAIETRTPLGALKRVLLSGSALAVIAAGSLEVSAQSLRDALAAA
                     YKTNPRLDAERARLRATDEEVARAHSGYRPTISGSADMGWERRETRPKSGGDGETHPH
                     GYALNMSQPVFRGFRTLNAVRIAEATVRAGRETLRTTEQSVLLEAVTAYMDVVRDTAI
                     VRLRENNVQVLTRELKATQDRFSVGEVTRTDVAQATARRAAAMSALDLARSNLQSSRA
                     TFERVIGFSPAGLVDPPPHSRNLPVSQSEAINIALARSPSVVAALYREQAARFTVEQI
                     WGELLPDVRLDASYTRRYDPSRTVDDSESATVTGRVNVPLYAAGETHARVRAAKHTHV
                     SRIQEIEQARTEARALAIAAWSRLVAARAQLQSDMAQVEANRTALAGVREEVKVGNRT
                     VLDVLNAEQDLLNSEVQLVTTKREIVVASYNLLSSIGRLEAAELAVTGTVYDPEAHYH
                     DVRRKWWGISITRPDGSRESHDLWPSHGVHSPVK"
BASE COUNT         4331 a         8187 c         8171 g         4406 t
ORIGIN      
        1 ccatcatcag gtgggtcgcg agatcgaggc cggcgtcgag atacgcaggg tcgactcccc
       61 gctcgaagcg gtagcgcgcc tctgtctgca ggccgagccg gcggccggtc gccgcggtct
      121 tcaggggatc gaaataggcg cactcgatca ggatgttctt cgtctcgttc gtcgagccgc
      181 tcgcctcgcc gccgagaatg ccgccgaggc cgagcacggc acgatcatcc gcgatcacgc
      241 acatcggcgg ctcgaccacg taactcttgc catcgaggcc cttgaagccc tcgccggggt
      301 tcgcaattcg ggcccggatc gtcccatgca gcttgtcggc gtcatagacg tgcagcggcc
      361 ggccgcggtc gaagctcacg taattggtga cgtcgacgac ggcgttgatg ggacgctgac
      421 cgatcgcttt cagtcgcgcc tgcatccagg cgggcgaggc gccgttcttg atgccgcgga
      481 tgtagcggcc gtagaacgcc gaacagccgc tcgcgtccgc gccgtcgacg tcgatcggta
      541 ccggacaatc gtagctgccc tcgaccttcg cgagcttcgg ctctgccttg agcttgccca
      601 tgccggcggc ggcgagatcg cgtgcgatgc cgcggatgcc cgtgcaatcc ggccggttgg
      661 cggtgagctt cacgtcgaga acggggtcgt cgaggccaag cgcgtcgatg tagcgtttgc
      721 caatgctcgt cgcgaacgtc tcgccgagat cgatgatgcc ctggtgatcg tcggagagct
      781 cgagctcgcg ctccgagacg agcatgccgt tcgagaccac gccgcgcacg gggcgcttgt
      841 cgagcgtgat cttggtgccg ggaatgtagg tgccgatcgg cgcaaacacg ccgatcatgc
      901 ccgtccggca attcggcgcg ccgcacacca cctcgacgac gcccgcgccc gtgtccacct
      961 gggcaacgcg cagcttgtcg gcgtcgggat gcttcttggc atcgagcacg cgagcgacga
     1021 tgaacggcgc gagcgctttc gccgggttct cgatgctctc gacctcgagg ccgatcatgg
     1081 agagcttggc ggcgagcgcc tccgcggtcg cgtctgtgtc gaggtggtca cgcagccagg
     1141 agagagtgag cttcatggga gtactcgcga acgtcgggcg caccgcgcct ggaattgtct
     1201 ttgaaccggc acttcggggt ccgactacga ggacactccg cccgccaagg tcggcacgtc
     1261 gagcggcaga aagccgtagt ggcgcatcca gcggaggtca gcagcgaaca tatcgcgcag
     1321 gtccgggatg ccatacttca gcatggcgat gcgatcgagg cccatgccga aggcgaagcc
     1381 ctggtacttt tccgggtcaa ggccgcagtt ggcgatcacg ttcgggtgca ccatgccgga
     1441 gccgaggatc tcgagccaat ccgagccctg cccgaacttg atctgcccgc ccgagcggtc
     1501 gcactggatg tcgacctcgg ccgacggctc agtgaacggg aaatgcgagg cacggaagcg
     1561 catattgaca ctgtcgacct cgaagaaggc ccggcagaac tcctcgacgc accacttgag
     1621 atgacccata tgggttgcct cgtcgatgac cagcgcctcg atctggtgga acatcggcgt
     1681 gtgcgtgcga tcgctgtcga gacgatagac gcggcccggc gcgatgatcc ggatgggcgg
     1741 cttctgcgac atcatcgtgc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgt cgtgctcctg
     1861 gcgcgctgga tgctccggcg ggatgttgag ctttgtgaag ttgaggtcgt cagtctcgat
     1921 gtccggcccc tcggcgaccg aaaatcccat gtcggcgaag atctcgatga tctcatcgta
     1981 aacctgcgag atcgggtgga tccgaccgtc cgccatgcca ccgagccgca cgggaagcgt
     2041 cacgtcggcc cgctcgcggg cgaggcgggc cgtgacctcg gcgcggccaa gctcgccctt
     2101 gcgcgcctcg atggcctcgg tgacgcgcac cttcacggcg ttgacggtct ggccgaaggt
     2161 cctgcgttcc tcgggcttca tggtgccgag cgaggcgagc agctccgaga tctgaccctt
     2221 cttcccgagc gcttgcaccc ttacggcctc gagcgacgcg aggtcggctg ccttggccac
     2281 gtcggcgaga agttgcgcct cgatcggcgc cggatcaatc gtagacgaca tggggcctcc
     2341 gggagttcgg gaaagtcagt gagaaccggc gtatctgagg gtatttcggc ggagactggc
     2401 agacgagcac ggctgcaaac gccaccggcc ggccaccctt cggcagccgg ctcatgtcga
     2461 gcgcgcctcg agcgggtgac gccgtcaggc cttggcggtc gcgggcatgg cggcctgagc
     2521 ctggccgaca agtgccttga atgcgcccgg gtccttcacg gcgatatcgg cgagcacctt
     2581 gcggtccacg gcgattccgg cctgctcgag gccgtgaata aatcggccgt aggtcatgcc
     2641 gtgctcacgc gccgccgcgt tgatgcgctg gatccagagc gcgcggaacg tgcgcttctt
     2701 gcgcttccgg tcgcgatagc cgtactgcag cgccttctcg acggcctgct tggcaacgcg
     2761 gatcgtattc ttccgccggc cgtagtagcc cttggcggcc ttcaaaacct tcttgtgctt
     2821 cgcatgagcg gtgacgccgc gcttgacgcg tgccatgggg aatctccgtc aaaggtgatt
     2881 ggtcaggggt aacgacagcc gaacccgcgc ttagcggtcg tagggcatgt acttcttgac
     2941 gatcttggcg tcgctgtcgt tcagcaccat ggtgccacgg gccgtgcgga tgaacttcgc
     3001 cgtccgcttg atcatgccgt ggcgcttgcc ttgggcctga gccttcacct tgccggtgcc
     3061 ggtcatcttg aagcgctttt tggcgctgct cttggtcttc agtttgggca tttggctcgt
     3121 cctttctggg accggacgcg gtcctgtgac ggatcgcgct atcagcggcc cgcagcttcg
     3181 cgcgaagcat cacgcgaagg gagccaaccg gtcagggtta agcagcaaag ccgccacggc
     3241 atgccccgcc gggcggcttc ggagcgcgtt atatgtggag ccgggtagaa ttgcaacaca
     3301 ctgccacccg accaggcagg cgagacgctt caggtaccgt tggcgctgtg aatttccggg
     3361 aaaaacagat ccttccacga ggtcggcttc gtcttgatgg tgccgacgcg gtgcatgaac
     3421 tcgacgaact tgccgacgtt gctcggcacc ttcgtgaact cgagaccctt acccatcatc
     3481 agctcgtcca tggtctccgg cgaggtgcgc ctgtcgttgg cggccaccag atagagccgg
     3541 ccggcggcct tcttgtcctt ggaaatcaga gcgagcgcct cgtcataggc ggcgttaacg
     3601 gcggcataga cggttggatt ctctgatcgg aacttgctgg tcgaggtcca cgtgaggccg
     3661 gtcgaaggtc cgcccatgat ctcgaagaag gtcgtgaccg tgcggatatt gggcgactta
     3721 agctcgacct cgttgaaggg cgaggttgca aagtgcgtat tgatttcgtg gacagggttc
     3781 aacactgcgg ccagcgcctc gggatgcggg agcggcacaa tgatgtggtc gagcttgttg
     3841 tgaccgccga cgccgaagac cttctccgcc gcgatttgca gcgcgagcgc ctgagtcgag
     3901 acgcgcagcg acggaatcgc gatccgatcc ttctcggtga aatcctttag tgatttgatg
     3961 ttcgggtttc gcgtattgag ccaaatatcg ttgttggcga tagcggctac agccttgatc
     4021 tcgcccttcg tccgatccca gatcaaggcc atcgacggca caccctgggc agcgaagtgc
     4081 aggctgcccg acaggaacgc gtcattgagt gccgccggac cgccgagctt cgcccacttc
     4141 actttgacgt cgcccatctt cttggcttcg aggtgcttct cgacgagctt caagtgctcc
     4201 atcagcacca tcggcaggta gacagcgccg aactgctgcc cgaggatcac ctccttgacc
     4261 tcggccctca cggccgatgt cgcggcggtc cccgccaagg ccagcgcggc gagcgcgcct
     4321 acgatccgtg ccttcatact ctgctctccc ttgcgcggga cttgattgat gacgcccgca
     4381 tgccggcatc ctagcaggct cggggtaacg cggtcacgcc catgcccggc atcgacagcg
     4441 cttggcaatg cgcctatagc tgcgccgatc ccgaaagccg gagacgctcg tgacccgtcc
     4501 agcacgccca gccgcaaagc caacgcagtt cgtcgacaac gagagggcga tcgtcaccga
     4561 gtggcggttt gcgccgggcg ccgagacggg ctggcaccgc cacggccacg attatgtcgt
     4621 cgtgccgatg acgaatggcg agctgctgct cgaggagccg ggcggcacat ctcggaccgc
     4681 gccgctgacc gcgggcgtgc cctacttcaa gtccgaaggc gtcgagcaca acgtcgtcaa
     4741 cgcgagctcg cacgaggtcg tcttcgtgga aatcgagttc aagcgtaagg cctgacgcgc
     4801 cgggcctcga agcgtcgccg tgacgccttg ttccacgcac tgtcgccgct attctcgccg
     4861 acaagatgag cagcactccc cgcggagacc ccgatggtcg acttcgccga gcaacgagaa
     4921 aacatggtcg agtcgcaggt gcggccgagc gacgtcacgg atcgccgcgt gttgcgtgcc
     4981 atggcccagg tgccgcgcga gcgcttcgtc acgagcgggc tgcgctcgat cgcctattca
     5041 gacggcccgg tgaaggtgtc tgatcagccg gtgcgctatc tgctggcgcc gcgcacgctc
     5101 ggcaagctcg tgcagctgct cgagatcggc gacagggacg tcgtgctcgt actcggctgc
     5161 tcgacgggct attcgacagc gatcatcgcc cgcgttgccc agtccgtcgt ggcggtcgag
     5221 gaggaagcgg cgctcgccga caaggcgcag acactgctcg ccgacctcgg catcaacaat
     5281 acggccgttg tcgcggggtc gctcacgtcg gggaaggcga gcgagggccc gttcgatgcg
     5341 atcttcatca acggctcggt cgaggaggtg cccgcggcca ttctcgatca gctgaaggac
     5401 ggcggacgcc tcgtgacggt cgagccccat gacggcgtcg gtcgcgcggt gcagtggcgc
     5461 cgctcgggca agacctatgg gcggcgcgtc gccttcgacg cggcagcccc ccgtttgagc
     5521 gagttcgaca agaagcgcga gttcgtgttc tgagcaatcg cggcgttgtt gatctggtct
     5581 tgcggtccgt ttggcgcagc ttcgcgatgc tggcagtgtc cgggataatc gccgccgcgc
     5641 cggcgggcgc aagccccccg gacaagcgct cgcccatacc tgcgggcctg cccgtcgccg
     5701 tcgacgtcat gggcgcgtct gagcccgtgc tgtgcgccga gaaggacaac gtgactgtga
     5761 acttcacggc gccgggcgta cgtgcattcc gcatcgaggc ggcccaccct gcctacatcg
     5821 gctcgctcgt cgccgacagt ttcgcagccg actgggccgc ctgcgacatg accggcgacc
     5881 ctgcgcatgc aggcgcggtc acggcgccga cccgccgaac catctacgag gaacctgaaa
     5941 tctggctcgt cggctggacg ttcccgacct tttggcgtcc ggcaacggcc acagtccgga
     6001 tcggcgatcg ggtggagcgc gggcttcatc tcgtccaggt ctggatgatc cgcccgatgg
     6061 gcggcgagga aatcctcgtc ttctatccgc aggacggcta ctggcgcgct cgcccgctcg
     6121 cgcccgccgg caaggcgccg acagcgttcg gctcctcgtt cctgctcggt cctgtcgaag
     6181 tcgaccgccg gccgatcgtg cgcctggacg agatcgcttt cgatccgaag gcgcgcagct
     6241 tcacgctgaa gttcgcccgc ggcggcagcg caaccctgag cctcgccgcc atcgaccaga
     6301 accggacgat cctcgacatc gccttcgatc gcgccatcga gggcgtaccg ttcgccgcgc
     6361 tccgctcgat gtacgtaacc gagacgaaca acgacgtcgc gcggatcggc atccgagcga
     6421 agggcgccaa gggctggtcg gaggagccga tcatgtcctt caagcgggca gcggccacgt
     6481 cggtgtgggc cggtcgcgtc gcgccatcgc ggcacaacac gagctctccc gacatgatct
     6541 tcgacagttt ccgcggcgcg ccacgataat cgtcagagct gggcgccgtc accccagttg
     6601 gccagcagcg cctggtaggc cggatcagaa tagcgcgtcg gcaggaacgc gggggcgtag
     6661 tccggcggcg cgagacccag catgtgccgc gcgacgagtt cgccgcccgc catgctcgac
     6721 atgatgccga agcccgaata gccgcaggaa aggtagattc cgtccacaga cgacggcccg
     6781 atcagtggcc ggttctcgcg cgtcttcatg taatagcccc cgtcgatgta ggggcggacg
     6841 cccttgtcga ggtaggcgcc gagcgccggc acgatcgtcg acatgccgcg cagtgcgatc
     6901 tcgccgtaat tctcatcttc ggcgagtgga tagacgacgt cgctcggctc gacgtgataa
     6961 ttgtagagga tgagcagcgt cgcgctctgc gggccgccat cgggacggcc gtgcacgccc
     7021 gcgggaaaag gctcggtgag ccagcgcatg gaatcgtcgg ccgcaagcgc ttcccgctcc
     7081 tccccattcc agggcaatcg ctggggatcg agccaaatca acatcggcgc atgacgcggc
     7141 accgcgccga gcgtgtcctg gatgctgatc ttcagatggc gctcggcgaa cagcggaagc
     7201 tcgatgccga gcgaggcggc gagcgggctg atcttgggcc cggctgcgag aaccagatgc
     7261 gtcgcctcga gattcgcccg tccctcgccg ctttcgatat tgacggagac gaggcgcccg
     7321 ccttgcgtct cggcgcctgt aactcggccg cgaagcgtgc gaacaccgtg ctcgcgcgcc
     7381 gcatcgagca tcgtcattcc gagttgctgc gcgctgagcc agcctgcgcg gcgcacatgc
     7441 gcgatgcaac gcgtctcggg cgcgagatac gggaagtact cgcggatcag ggcgcgatcg
     7501 gtgatgacgt cggcgccggt cagcggactc tcgaagccat gagctgcagc cgacacgtag
     7561 gaccggctgc cggccccgtg gacccgtacg gcacccgccc cacgcgcctc ggcgacccgc
     7621 gcgttctcta tcagccaatc ggccttggcc ggatcggcgg aggcgaacac gtagccgcga
     7681 cggttgagat tgaaccggtt tccggtcgtc tgcgcgagac cctcgatgag gtcaatgctg
     7741 cggttcatga aggcggtcat ggcctgatcg ggacccggcc accagttgcg ataggcctca
     7801 gtcgacttgt cggaggtgag cgacagcgga ttgccctcct cgacgagtac gacattgcgg
     7861 cagccatgca cgacggcgag ttggtagacc gccgcaacgc ccgcgatgcc ggccccgcag
     7921 atcacgaact tcgccgtcgc aacgctcata gctgcatcct tcctgccgcc gtggcgcccc
     7981 cggacggagc cgccgtcaca cgtcgctcct atagaggtag atcgggctcg accaggcgcg
     8041 atgcccatcc tcctgggtga cacgcacgta aagccgcgta tcaccggtgt cgctgaggcg
     8101 gatcttgcgc cggagcgtca cgcggcgctc gctcatgcga tcggggaggc gatagaaccg
     8161 caccgcacga tcgagcccgc ctccctcgaa ggtgcgatcc tcgaggccga tctgggcgag
     8221 cacaaggatg ccctgacagt gcgcggtctc gaaggcgagc cggccctcgc gcgccgcctc
     8281 gaggcgaatg tcgatgccgc cgaaattgcc ggtcgtcacc gctttccatt cgaggctcgt
     8341 gcggctctgc ttctggatgc cgcgatcgag gttccagttg ttcagcaccg aggcactggc
     8401 gatgccgttc gcctcgagct cgagcacacc gtcccagatc gtggtgcgcg cgcgaccgcg
     8461 gtactcagcc ccctgataga gaagccggat tcgtgcacct agatcgctat cgctgaacgg
     8521 ccgataggtc tcgatcagct tcgtggcgtc gaaaacgtcc acccgctcga tcggcgccga
     8581 accaacgacg tcgatgacta gatcgacctc ggcatcggcc acctcggcga tgtcgcccat
     8641 gatcatgtgg cgcgtgtcgg caatgcgcgt atcggggaag tggtcgggat cgcgctcgaa
     8701 aagcaccgca ggtcgcgccg tcgtcacctc gacggcgagg ctcatccggt tccctgtcgt
     8761 cgcatagtga tgccggcgtc gcatcgcctc gaagacggca tcgcgatcga gccgcggggc
     8821 caggaagcag gtgagtccgc ccatcgagcc gaagaaggag gcgccgggcg tgcacgcgcc
     8881 gggccggccc ttgtggccat ccgagttggc gacgatgccg acgcgatacc ccttcgcgaa
     8941 ggcatcgtgc aggagccact cgaatgtgcc ccacgccgaa tgcacctcga ctgcggtctc
     9001 gagacggccg tcatgggcga gcgtgatgtc cgcatagcga ccgccaacgt gcgcggcgac
     9061 gacgcaatcg cgccctgcga gcgcgcgaaa gagatcggcc gccgtatggg cgtcggtgtg
     9121 ctcgtcgacc atgtcgccga cgtcggcgat ctgggcgtgg ctcgagcgat ggatggtctc
     9181 gccctcgtgc cggaagtgca cgttgcgatc gccgccgacc gccgtgttgg cgctccattc
     9241 gtagccggga atggcgacga agcggcccgg cttgtcgagc gaacgcgtca gctcgttgag
     9301 gtcgcgccag aatgcgccgg tgatctggaa gtcgttcccc tggtggccca tcacgtcaag
     9361 gaaggcgcga tcgcggccga actcgaaata ctcgcgcgcc gatcccgtgc cgagcgtctc
     9421 gtctgattgc ccgtgcgtgt caccccagta gtgcacgagc gacgcatctg ttggtgcgac
     9481 gtggagcgta ttggagcgga cgagctcgcg gccgtccgtc gtcttcaccg cgatctggag
     9541 ttcgccatcg tcgtccagcg tgaggccgtc tacaatcgct gactgcgccc ctggcgcgaa
     9601 ttcgatcgta cgcggcaggc cggcaaccgg ccggctcggc tcgacgatca gcgagcgcgg
     9661 cacatgcggc gagggattgc cccacttgtc atcagcccga acgccgaagc ggaacgcttc
     9721 gcccgagcga acaagcgtcg gaagcacggc gcgccactgc acgccggatc ccgccacgat
     9781 ggcaatcttc gggctctcgg gcagcgcgac gtagtcgtag gttgcgatcg catcgacgag
     9841 aacgtggaac tcgaaagccc gctccgcata ggtctggagc cggatacccg gcgagccctg
     9901 tcggcgatca ccgaagcgga tcgtgatcgt atcgccgggc ttcaggaagt gcttcatgac
     9961 gccgacatag agcgagcggc tccaggggcg gatgttgcgc ttgaactccc acttgagctc
    10021 gagagtcgcg ccgttcgatg cctcggcagt cgtgtatcca gccgcctcag gcttgtcgaa
    10081 ctgcacgggg ccgaagtcgg ttgcgaaacg gaagccgatc ttgatcgagc ccgtatcgtc
    10141 gatgccgaac ttgcccgccg tgtagacgag cgtgaacgac tgcatcgagc cggcctcgaa
    10201 ctggccggtg ggctcgagcg tcgcgcggcc catcagctcg ggccggtagt cggaataggg
    10261 catgggattg cttcaagcgt gatcgtgagc gcgggctcac gcggatgcac tcgttggttc
    10321 gatgcaaact ggaccgagcc tcgaccggaa tgtcaaggtc ggcgcattcc ggtctgccac
    10381 cgttcgactt tcagacgaca aatccgcccc aaggagcccc ggggcggatc gccgttgtcc
    10441 aaagggtgcc gggttcactg acccgggaac ttgtagtcga gcaggcgggc attcatgtag
    10501 ccctgctcag cgatgcggtt ccaggccatc agctcgcgac gcgccttgat gtaggacgcc
    10561 gccgtctcct tggtgagctc gtccttgtcg tcgagcagct cctggatgac ctggccggac
    10621 gcggcaccgt aagccgcgag gaagtcgttc ggcagccgct tcaacgtgac attgtgcgtc
    10681 ttgatgagcg tctcgagcgc ctggccgttg cgggcgtcga actcggccgt gaggaggcgc
    10741 gattcggcgc gcgcgcactc cgtgaagatg cgctgcacgt cggccggaag tgctgtgaac
    10801 ttcttcttgt cgaccgtgat ctcgagcgtg gtgcccggct cgtgcatgcc cggccagtag
    10861 tagaacttgg cgaccttgta gaagccgaag gcgaggtcgt tgtagggacc gacccactcg
    10921 gtcgcgtcga tggcaccaga ctggagagcc gcgaagatct cgccgccggg caggttgacg
    10981 gtcgtcatgc cgagcttctt cagggcctcg ccgccgagac ccggaatgcg catcttcatg
    11041 cctttgacgc tgtcgacgga ggtgatctcc ttgttgaacc agccggccat ctgcacgcca
    11101 gtcgagccgc cgtggaagcc cttgaggccg aacttgtcga ggatcttgtc ccaaatctgc
    11161 tggccgccgc cgaactccat ccaggccgag gtctcgttgc cggtgaggcc gaacggcacg
    11221 gccgagaaga actgcaggcc cttgttcttn nnnnnnnnnn nnnnnnnnnn nnnagtatgg
    11281 agcggcgtgc atcaggtcag cggcgccgcg cgtgatggca tcgaagctct cgaagggcgg
    11341 cacgagctcg ccggccgcga acaccttgat ggtgagacgg ccatccgtcg ccgccgtcac
    11401 agactcggcg aatcggttgg cgcccgtgcc gaggccgggg aagttcttcg gccaggtcgt
    11461 caccatcttc cactcggtct tggcctgagc gatggctggc gtgacgatcg cagacgtacc
    11521 ggcgccggca ccgacgactg cggcacctgc cagaaactga cggcgcctta cgcgtgtctc
    11581 gcgcttctcg gatttcatgg tctctctccc tgactggccc gacgtgaggc tgcactcgct
    11641 tagcgcgact tgtggtcctg gccaagcttg ctcgagcgct ggatgccttg ccaaagcggc
    11701 gtgactgtgg ctccggtgcc gcacgacaag ccagattgac gaatatcagg gcacccgccc
    11761 atgcggccat tgcgccacat gggtcgtcat tcgtgcggtc ggaccccgga tcaccttgcc
    11821 gccttggctc accacgctta gccctcgctc ttcagcagca aatcgaggaa tccaatcgat
    11881 gacgtttcca gcgctcgcag cggccgcggc cgtcctcacc ctcggcattc ccgccatcaa
    11941 cgcttcggcg caggacgctc cgaaaaaggc gaaagctgcc gccaaggccg ccccggtcaa
    12001 ggcaaactgc acgaccaggt acggtaaggg ctacgcctgg accgagagca tggcgcgatt
    12061 ccaggcctgg gagatcattg cccagacgac cggaaactgg ccgatcatga tggataagtt
    12121 ccgcaacgag gtctacaagt gcaagccgga tggaagcggc ttcacgtgcc actccaagat
    12181 cgacgtctgc aagtcctgaa caccgcgcat cgacgcgaaa ttgcccgccg gcgcccagtg
    12241 gcgatgacgg gccatccgga ggccgccggc catgctgctc gaggacaagg tcgcgatcgt
    12301 gaccggcgcg gctcagggca tcggcaaggc gtgcgcgatg cggctcgccg ccgaaggcgc
    12361 caaggttgtg attgcagacg tgaacgagga gcaaggggca gcgctcgtgg caacgctcaa
    12421 ggagtcgagc cacgacgcga tcttcgtatc atgtgatgtg gccgagcgcc tcgaggtgca
    12481 caatctcgtc gccgcgacgc tcgatgcctt cggtcgcgtc gacgcccttg tcaacaatgc
    12541 cgccgtgatc gatgacgtgg cctttctcga gctcgacgag gcagagttcg accgggtgct
    12601 cgacgtcaac ctgaaaggcg cttttctcgt cggccaggcc tgcgccaagc agatggtgaa
    12661 gcaggcagcg aagcagtcgc gcggatccgc cggcgcgatc gttaacatgt cgtcggtgaa
    12721 cgagaatttc gcgctgccag accatgtcgc ctatacgatc tctaaaggcg gcatcgctca
    12781 gctgaccaaa tcgatggcgc tggcgctcgc cccttaccgc atccgcgtca atgcggtcgg
    12841 tcccggcact atcgagacgc ccatcaccgc gagcgtgatc cgcgacgaag ccatgcgcgc
    12901 caaggcgctc tcgcggacgc cgctcggacg gttcggacgg ccagaggaag tggcggcggt
    12961 tgtcgcgttc ctgctgtcgg acgaggcgag ctacgtcacc ggcaccaccg tgttcgtcga
    13021 cggtggccgc cttccgctga acttcgtgat gccgacggcc gacaaatcgc gaaccgagcc
    13081 ggcggacgac gaggacggct gatcgagccc gcgcgccgac tacccccatc agccgagctt
    13141 caaggcctcg atgcgcgccc gcgcgaacgc cggccgcgcc gttagccgct gccaccagtc
    13201 tcggacacga gggtagcctt cgagcgtcag gcccagctcg gccgtctcgc cgatacgcac
    13261 gatgtagggc gatagcgcga tgtcggcgag cgtgaacatg tcgcctgcga gccaggggcc
    13321 gtcgccgaga tcacgctcca ttttgtcgag gcagtgcctg atgccggcca cggcgcgttg
    13381 gcgctccgcc tcggtgaagg gttcgcgggc aacgcggcgc caggcctcgg cgcgctcggg
    13441 catcggggtc cgtgcgagct tgtcgccgag ctggctctcg ctccagcggc tcgcagtcgg
    13501 ccccatcatg cggttccaat tgagcaccat gaacagcttg atgacgtcgg tatcgacgta
    13561 ctgcgtccac acccgcatgt gcgcccgcgt caagggatcg accgggcgga gcgcacgatc
    13621 cggaaagacg tcctcgagat actcattgat gacactgctt tcgatgacga cgcgcccgtg
    13681 atgcacgagc gtcggcacaa caccattcgg attgagcttc aggtagtccg gcgtcagttg
    13741 ctcgttctcg agcagcttca cgacgacgcc cgtccaggca aggcccttct cctcgagggc
    13801 aaagcggacc ttgcgcgagg cgctcgaagc ccaggcatgg tagaggacga gttcgaattt
    13861 ggggacggga tcagtgaccg atgtcatggc agctccaaac gattgccggc atctctcatt
    13921 gtagcctcct caccagccca tgaaagagga ccatcgctat cgggggcggc ggagatcagt
    13981 catctcgatt gttacgtctc ggcacgacgc atcccggtac gacacacatc ccggtacgac
    14041 gctgtgcacg agcggcgctt cgggataacc tcgacgcgcg acgaccggag ccccccatgc
    14101 gctatgcatt catactgttg ttcatggcgg tgccactcgc tgagatcgca ctcctcgtca
    14161 agttcggcca ggtgttcggt ttctgggcga cgatcggcct ggtcgtcggc accgcgatcc
    14221 tcggcggcgc cgtacttcat tcgcagggct tgcgcgcgtt gacgcgcatc caggcgtcag
    14281 ccgagaaggg tgaggcaccc atcggtccgg tgatcgacgc cggcttcatc gcgctcgcgg
    14341 gatttctcct ggtgacgccc ggggtgataa ccgattcaat cggtctcctg ctgctcatcc
    14401 cgccgatccg acggcgtatc gccctcgtcg tcgtgcgctg ggccttacgc aactccgaga
    14461 tcagggtcga gacgtttcgc cgtgagcgcg cgcgtacgcc gccgcctggc gacgagatga
    14521 gcgatggtcc cgtcatcgac gtcaaggccg agcgcctcga cgagcggccg atgaaaccga
    14581 caggcagcga caacgggtca ggccgaccca accattgacc ggagcagcac gcgttgcgcc
    14641 aaagtggcga tgcatgatag gcgcctcatg aaccagcggg gagagcgagc ccatgaccga
    14701 cacgcccaac accaagccga cggcccaggc cactccacct gccggtgcca agacggacgg
    14761 tcagccggcc aatgtcacgg cccgcgcggt cgcccagtac atccgcgacc tgtccttcga
    14821 gaatcccagc gtcgagaagc agattgcctc gcagcccgag gcgccgcaga tgaagatcga
    14881 gctcaatgtc agcggcaagc cgatgggcaa ggatctctat gagagctcga tcgagctgaa
    14941 agccacggcc tcgggaaagg tcgggacgat ttacattctt gaactcacat acgctggtct
    15001 cttcaagctc gagaacattc cgccgcaggc gctcgagccg atcctgctcg tgaactgccc
    15061 catgttgatt ttcccgttcg cgcgccgggt cgtcgccgat ctcacgcggg agggcggcta
    15121 cccgccactg ctgctcgacc ccgtcgattt cgcccagctc tacttcgcca agcagaagca
    15181 ggtcgccgcg gccaaggcgg gcggcggcac gccacccgtt gccggacgtg cctgagcgcc
    15241 gtcagctgtc gcgcttcttc caaagcgcat tcggcccgag cgtctcgacg aatttcgcat
    15301 gctccgcctc ggcctctgcg gtgagccgcg gcgcaagagg ccgtgtgcgg gccttggcgc
    15361 cgccgctctt ctgctggccg cgccccgatg tgccgcgggc cggtccgccg accaaggcga
    15421 gcgtcgtctg gcgctccccg agcagctcca tgtagacctc cgcgagcagc agcgaatcca
    15481 ccagggcgcc gtgcttcgtg cgcttcgagt tgtcgattcc gaaccgtttg catagcgcat
    15541 cgagcgagtt cggcccgccc ggattgcgcc ttctcgccaa cgccaacgta tcgacgacgc
    15601 gcgtcatctg cagcggcttc ctcttcaccc gctcgagctc gctgttgatg aagccgacgt
    15661 cgaaagtggc attgtgaatg acgagcggcg catccgcgat gaaggcgagg aattcgtcca
    15721 ccacagccgc gaacttgggc ttgtccgcga ggaacgcgcc cgtgatgcca tgcacggcga
    15781 cggcatcggc cggcacatcg cgctccggat tgatgaaggc gtgaaactcg cggccggtcg
    15841 gcagtcggcc catgagttcg acgcagccga tctcgatcag gcgatgcccc gccctcggat
    15901 cgatgccggt cgtctcggtg tcgagcacga tctcgcgagc tgtcatgtgt ccggctcctt
    15961 ctcgtctcag gcccagtggc gctcgtaggc atcgcctttt cgcccgcgca gctccgcaac
    16021 gatcttatcg actgcggcct cgctctcggg gatagagccg cccgtatcca caacgtagtc
    16081 ggcgcggcgg cgcttttctg catccggcat ctggcgggcg aggatcgcgt cgagcttttc
    16141 gggcgtcatg ccgggcctgg cgagcacgcg ggcacgctgc gcctccgccg gcgcgctgac
    16201 gacgaccgtc acgtcgacgc gctcggctcc gcccgtctcg aatagaagcg gcacttcgat
    16261 caccgcaagc tggtcgccgc gggcggcagc ggcacgcaac tgcgcgcgct ccgcctcgag
    16321 gacgagcgga tggacgatgg cattcagctt cttgaagccc gacgagtccg ccaggagctt
    16381 cgccgaaagc ctttggcgat cgacgcgacc attgcctgtc gttcctggaa aagccgcctc
    16441 gatcagcggc acagcggcac cctcatagag cgtgtgcacc tcggcatcgg cgtcgacgac
    16501 gagcagcccg tgatgccgga aacgcccggc cgcggtcgat ttccccatac cgatggagcc
    16561 ggtcagtcca atgagcagca tccctggtcc tcgtcgcagc ctgttcaaat gtcgagcatg
    16621 ccgcgctcgc gcagcgcgcc gagcaaaggc agcagcggca tgccgagcac cgtgaagtag
    16681 tcaccctcga tggcctcgaa gaggcggacg ccgaaccctt cgagctcgta gcacccgacc
    16741 gagctgagaa cccgcgatcc aacacgacca aggtaggcgt cgatctcgtc ggtcgagagg
    16801 cggtgcatcg caagccgggc cgtcgcgacg taccgccaga ccggcctgcc cccgctcgcg
    16861 atcactgctg ccgaatgcag ctcgtgtgtg cgcccggcga ggcgcccgag ggccagccgc
    16921 gctccctgca agtcgggtgc cttatggagg atcgcgccat cgaatgcgag cacttgatcc
    16981 gagccgatca cgactgcatt cgggtggcgc acggagacct cgatcgcctt cgcttccgcc
    17041 aggatcgcgg cgactgtgct tggaggcgac gcatcgttcg cctgctcgat cgcacgctcg
    17101 tcgatgtttg cgggtatcac ctcgaagtcg acaccggcag tgtcgagcat cgttcgccgc
    17161 gccgcgctcg ccgatgcgag aatgaggcgc cggggcgccg tgctcgactt tgaccgggcc
    17221 tcagccatcg tcggtctcgg cgtcgatggc gcgcgtgtgg cggtcgttgt acatcttgat
    17281 gatcgtcgcc gccgtctcct cgatcgagcg gcgcgtcacg tcgatcatcg gccagccgta
    17341 cttcccatag agtcgcttgg agtaggcgat ctcggcggcg atctgctcct tgtccgcata
    17401 ggtctcgata tcgcgatcgg agagcaggcg aacccggttg cgacggatct cggcgatgcg
    17461 gtcgacgctc gcgacgaggc cgacaacaaa cgtgttgtgc gggcgcgtca gctgctcggg
    17521 gagcgcgatc gacgggacca gcggcacatt cgtcgtcttg tagccgcgct gcgcgagata
    17581 gatgctcgtc ggggtcttgg aggtgcgcga gatgccgacg atgacgatgt ccgccgtgct
    17641 gatgtcggcg gggagcttgc cgtcatcgtg ggccatggtg aagttcaagg cctcgatccg
    17701 cttgaagtag ccggcatcga gcacgtgctg gccgccgacg atgggcgtct tggcggcgcc
    17761 gagatacgat tcgaacaccc gcatgatcgg ctcgagcgca tgcagcgaag gaacgttcag
    17821 ctccttgcag cgtgcttcca gagatgcgga gagttcggag ttcaccatcg tgtagagcac
    17881 gatgccgggc gttgaggcga cctcctggag gacgcggtcg agctggcgct gcgagcgaat
    17941 aagaggatgc atatgctcga tcgggcgcac ctgggcgtac tgcgccgcgg ccgccttggc
    18001 gatcattgtc agtgtctcgc cggtcgaatc cgacacgaga tgcaggtgga agtagctggc
    18061 gggccgctgg gtcatgtagt gccgttggct cgtctgtgga gaagaggtgc ggttatccac
    18121 gccaccgggc gtcgggcggc cgtgccaata acaagtagcg ccttttcaac agagtcgtcc
    18181 acgcgagcac tttcgctgcg acagaaatgc agcccgggca ggagcagacg caacgcgaaa
    18241 atcgcgagcc cgatatcatc atgttgtcga cagccgatcc acaacccagg ccgcgtcgta
    18301 cgcgctatcg atgtccttgt gacctcagtc gaattggctc caccttgtgg tctgcctgct
    18361 cgcgacatac aagatgcggg ctcgatgccg gggatcgcgc gttccatggg gacaagcggc
    18421 gtgcaagcag ctgcccccga agtacacccc ggtaaagagc aataacaata atctttcaga
    18481 ctctgtcttg agattcagaa gggcagaacc ggtgagcgct tcgaagccga cacgtcactt
    18541 tctcaagccc tttcagggca gggcgacggc gccaccgccg atctggctca tgcgccaggc
    18601 tggccgctat ctgcctgagt accgggcgac gcgcgagaag gccggcggct tcttgaagct
    18661 cgtctacagt cccgagcttg ccgccgaggt cacgctgcag ccgatacgcc gctacggcct
    18721 ggatgcggcg atcctcttct ccgatatcct ggttgtgccc gacgccctcg gccaaggcgt
    18781 gcgcttcgtc gagggtgagg gaccgcgtct cgaccccatc agggaaccgg ccgacattgc
    18841 ccgcctgagg ccgaaggggg ccggctccaa gttcgaaatt gtcgctgcga cggtcgcccg
    18901 gctccgccag gatctgccgc gtgaaaccgc cctcatcggc ttctgcggag caccctggac
    18961 ggttgcaaca tacatgatcg ccggcgaagg ctcgaccgat caggccgacg cccgcggttt
    19021 cgcatatcgc gatcccaacg cgatgcaggc gctgatggac atccttgtcg ccgcctcgat
    19081 cgagtaccta tcgctgcagg tgaaggccgg cgcggatgcg cttcagatct tcgatacgtg
    19141 ggccggcagc ctcgccgaag accagtacga acgctgggtg atcagcccga cactcgccat
    19201 caccacggcc atccgcgcgc gacacccggg cgtgcccatc atcggcttcc cgcgcggggc
    19261 tcacgcgagt gcttcgacct acctgcaatt cgccggcgtc gacggcatca gctgcgatac
    19321 gtccatgccg ctttcggcga tgagcaggct cgcagagggc ggcaaagtcg tgcagggtaa
    19381 tctcgacccg ctccttctcg tcgccggggg agaggcgctc gagcgtcgcg cgcgtgaggt
    19441 cgtggagacg atggcgggcc tgccattcgt gttcaatctc ggacatggca tcgttccgca
    19501 aacgccgccc gagaatgtag cccgcctcgt cgaggtggtt cgcggggcgg gagcgcaatg
    19561 acggacctgc tggtcaagtc gctccacgtc gtcgccgtca tatcctggat ggccggactg
    19621 ctctacctgc cgcgcctgtt cgtctaccac tgcggcgcgc gccctgggtc cgagcaatcc
    19681 gagaccttca aggtgatgga gcggcgcctc tacaaggcca tcatgaaccc agccatgatg
    19741 gtggtctggc tgacaggccc ctggctcgct tggcgcttgg gtcacctcga cgagcgctgg
    19801 ttctgggcca agatgctcat ggtttatgcg ctgacgtggt accaccacgt cctgctcgga
    19861 catcttcgca cgtttgccgc cgaccggaac accagggaag cgcgccactt ccgcataatc
    19921 aatgaaatcc cgacgctgct gatgatagtt atcgttgtat tggtcatcgt acggccatcc
    19981 ttcaggtttt agatcaatct cacggtgaca cctgaggttg ccgccctgcc gcaagtgtgg
    20041 actgcaaatg tggactttgc ggacaccgag ggttctgcta tacacggcgt atccgggact
    20101 ggatcgagtc ggcctcactg ccggctgcag ccgcgccttg gcgggttccc tcacgatggc
    20161 gctgagacca gttctcctcg aggactgccc ggattcccac cttcagatct cgctcggccg
    20221 ataatgaacc cgcgacctcc gcccgagccc gcacacgagt ctcacaatgg acaacgatat
    20281 tacagccgcc gctccggatg ccccggaagc acctgccgtc aaggaaatga aacttcagga
    20341 cctgaagtcc aagaccccga cggagcttct ggcctttgcc gaggaggtcg gtgtcgagaa
    20401 cgccagcgcc atgcgcaagc aggagctgat gttcgcgatc ctgaagcagc ttgccgaaaa
    20461 agggatcgag atcataggca cgggtgtcgt cgaggtgctc tcggacggct tcggcttcct
    20521 gcgctcgccg gacgccaact atcttccggg tccggacgac atctacgtct cgccctccca
    20581 gatccgccgc ttcgcactgc gtaccggtga cacggtcgag ggccacatcc gcggtccgaa
    20641 ggatggcgag cgctatttcg cgctcctcaa ggtcaacacg atcaacttcg acgagcccga
    20701 gaagcagaag cacaaggtcc atttcgacaa cctgacgccg ctttacccga ccagggcctt
    20761 gaagctcgag atcgaggatc cgacgatcaa ggatctttcc gcccgcgtca ttgacatcgt
    20821 cgccccgctc ggcaagggcc agcgcgcact gatcgtcgct ccgccgcgta ccggcaagac
    20881 ggtgatgctg cagaacatcg ccaaatcgat cacggcgaac caccctgagg tcttcctgat
    20941 cgtgctgctc atcgacgagc ggccggagga ggtgaccgac atgcagcgct cggtgaaggg
    21001 cgaggtgatc tcatcgacct tcgacgagcc gccgagccgg cacgtgcagg tctcggaaat
    21061 ggtgatcgag aaggcgaagc gcctcgtaga gcacaagcgc gacgtggtca tcctgctcga
    21121 ttcgatcacc cgcctcggcc gcgcctacaa caccgtcgtg ccgagctcgg gcaaggtgtt
    21181 gaccggtggc gtcgactcca acgcgctcca gcgcccgaag cggttcttcg gcgcggcccg
    21241 caacatcgag gagggcggct cgctgacgat catcgccacc gcgctcgtcg agaccggctc
    21301 gcgcatggac gaggtgatct tcgaggagtt caagggcacc ggcaactccg agatcatcct
    21361 ggaccgcaag gtctccgaca agcgcgtctt cccggcgatc gatgttgccc gatccggcac
    21421 gcgcaaggag gacctgctcg ttccgcagag ccagctgcgc aaggtctatg tgctgcgccg
    21481 catcctcaat ccgatgggca ccatggatgc gatcgaattc ctggtcgaca agctgcagaa
    21541 atccaagggc aacggcgact tcttccagtc gatgaatacg ccgaactcgt gaggccgaga
    21601 ccggcccctt tacctctcat ttagcgaatc agtcgcgaat ggacgcgccg ggcctcgtgc
    21661 ccggcgtgat ccgtttcggc gcgcccgtgc ggtcacgacc gacgtgtcac ctcgccgtcg
    21721 gcggggagga gatcgcatgc gcttgggcag tctggttcga tcattgcgtc tgagggtgcc
    21781 ggggctcgac ctgggccgcc gcagccggct gctcgccgcc tcggccgtgg cgctcacgtt
    21841 cagcgtcgtc gcacatacgg gcgagggttt tggccagacg ctgcgtcagt ctcttgcgaa
    21901 tgcctacaag aacaacccgc gcctcgatgc cgagcgtgcg cgcctcagag ccaccgacga
    21961 ggaggtcgca cgcgccaact ccggctggcg gccgaacgtg accggctcgg cggatgtcgg
    22021 caagcagacc gtgcacgtcg tgccgaagtc gacggccgat ggcaactcct cgccgtgggg
    22081 ctattctgtg agcgtcacgc agccggtgtt ctcgggcttc cgcacggtga acgccgtgcg
    22141 cgaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tcgtcaagct
    22261 gcgtgagaac cacgtgagcg tgctctctcg tgatctcgct gcgacggaag cccgccgcaa
    22321 tgcgcgggag gtgacccgca ctgacgtcgc gcaggccatg gcccggcgcg ccaaggctgt
    22381 atcggcgctc gatctcgcac gtgccaacct gaaatcctcg cgcgcggcct tcgaaaaggc
    22441 gacgggcacg gcaccgggcg gcctcgtcga gccgcaaccg ctcttcaagc ttctgccacg
    22501 gtcgctcgaa gacgcactgc gcatcgcgga ggcggaaagc ccgaacgtcg tgtcggccct
    22561 ctatcgtgag acggcggcac gttatgcggt cgagcgtatc tggggcgaat tgctaccgga
    22621 nnnnnnnnnn gtgcggctcg aggcaagcta ccagcatcgt ctcaaccctg gcgcgagtgt
    22681 cgatgagcag gatgtcgcca gcgtcaccgg ccgcgtgaac atccccctct acgccggcgg
    22741 cgagacgcac gcgcggatcc gggcggccaa gcacactcac gtgagccgca ttcaggagat
    22801 cgagcaagcc aagaacgagg tgatggcgct cgtcactcaa gcctggtcgc ggttccaggc
    22861 ggcgcgcggc cagctcaagt ccgacaaggc ccaggtggag gccagccgga ttgcacttga
    22921 aggtacccgc gaggaagaga aggtcggcca gcggacgatc ctggacgtcc taaacgcgga
    22981 acaggagctg ctcgacgctg aggttcagct cgccacgacg cggcgtgacg tggtcgtcgc
    23041 cgcgttccag ctgcttgccg tgatgggcaa gctctcggcg accgacctgc acctctcgga
    23101 cacggtctac gatcccgacg cacactatgc cgaggtgcgc cgcaaatggt tcgggatcga
    23161 tattacgcac gcggacggac gcctcgaggc cgtggatgca accgacgttc acggtccgag
    23221 ctgggacgac gacggagagt gaagtggtgt gcgacgcggc acggaatgtg ttgatatcgg
    23281 gcaacgctgc tcgaacaacc cgacgcatcc tgcgcattca agcgccgtgg ggccggctct
    23341 ggcgccccta acacgttgtt aactatagtc gccgtgtggc actaatgccg tgcgattcta
    23401 tcacgcgtgg gtgctccgga ttcgaagtgg agtcggtcgg agcctgatgc aaagcacgag
    23461 cggcggttca tcatggctat cgaaacgcgg acgcctctcg gggcgttgaa gcgggtactt
    23521 ctgtcgggtt cggctttggc tgtgatcgca gccggcagcc tcgaggtgag cgcccagtcg
    23581 ctgcgcgatg cgctggcggc ggcctacaag acgaaccccc gcctcgacgc cgagcgtgcg
    23641 cgccttaggg caacggacga ggaggtggcg cgagcccact ccgggtaccg tccgaccatc
    23701 agcggatcgg ccgatatggg ctgggagcgg cgcgagacgc ggcccaagtc cggtggcgat
    23761 ggcgagaccc acccgcacgg ctacgcgcta aacatgtctc agccggtgtt tcgcgggttt
    23821 cggacgttga acgccgttcg gatcgccgag gcaacggtgc gcgcaggtcg ggagacgctg
    23881 cgtacgaccg agcagtcggt gctcctcgaa gccgtcactg cctacatgga tgtggtgcgc
    23941 gacaccgcca tcgtcaggct tcgcgagaac aacgtgcagg ttctcacccg cgagctgaag
    24001 gcgacacagg atcgcttctc ggtcggcgag gtgacgcgca ctgacgtggc ccaagccacg
    24061 gcaaggcgcg ccgcggccat gtccgcgctc gatctggccc ggtccaacct gcagtcgagc
    24121 cgcgcaacct tcgagcgcgt catcggtttt tcgccggcag gtctggtaga ccctccgccg
    24181 cattctcgaa atctgccggt gtcgcagagc gaggctatca atattgcgct ggcgcgctcg
    24241 ccgagcgtgg tcgcggcgct ctatcgcgag caggcggcac gcttcacggt cgagcagatc
    24301 tggggtgagc tgctccccga tgtacgtctc gacgcaagct atactcgccg ctatgacccg
    24361 agccgcactg tcgacgatag cgagtcggcg actgtcaccg gccgtgtgaa cgtgcccctc
    24421 tatgcggccg gcgagacgca tgcccgcgtg cgcgccgcca agcacaccca cgtgagccgc
    24481 attcaggaaa tcgagcaggc tcggaccgaa gcgcgcgctc ttgccattgc ggcctggtcg
    24541 cgtctcgttg ccgcccgtgc gcagcttcag tcggatatgg cgcaggtgga agccaaccgc
    24601 acggcgctcg ccggcgtacg cgaggaagtg aaggtcggca accgcaccgt cctcgatgtt
    24661 ctcaatgccg agcaggacct tctgaattcc gaagtccagt tggtcacgac caagcgcgag
    24721 atcgtggtcg cttcctacaa tctgctctcc tcgatcggcc gcctcgaggc ggccgagctt
    24781 gccgtgaccg gcaccgtgta cgatccagag gcccactatc acgacgtgcg ccgcaaatgg
    24841 tgggggatca gcatcacgcg gccggacggc agccgcgaaa gccacgacct ctggccgtcg
    24901 cacggcgtcc acagcccggt gaagtgacag attctttttt tccctcgagc agtgcctcat
    24961 attctgcctt cactccgcca aggacttccc tgatcctgac gggaaagtcg gtcgaaagcc
    25021 cggcaccggc ggaacgtgcc gcgttttccg ggccgggatc gggtccgtga gcaggcgttg
    25081 catcgcgttg atgcagttcc ctattcggtg tacgagggcc gatcacatgc tgcctacgcg
    25141 cgggcggggc gactgcgccg tgcgcccgct ggatgcgcag tggcaatcgt tcgacgagag
    25201 ttagaggctc gctatgacga caggctcgca gaaagttaca gagccgagca tggacgacat
    25261 cctggcgtcg atccgcaaga tcattgcgga ggagccgacg aagactccgg tcatctc
//
LOCUS       DAORNH010000024        25317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1507_length_25317_cov_2.400523, whole genome shotgun sequence.
ACCESSION   DAORNH010000024 DAORNH010000000
VERSION     DAORNH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 25317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25317
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1507_length_25317_cov_2.400523"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2984)
                     /locus_tag="PK264_04325"
     CDS             complement(<1..2984)
                     /locus_tag="PK264_04325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRD75153.1"
                     /translation="MTARSDRLSVDRGIYSRGGRLALLVVAVVALLALSTAQASAQPI
                     QAHAIDPTLDRIDVTAIGRSIEAGGDKLHVDMAPGTDGTGGRMTVAATTQSTSWFVFA
                     IRNTTDKPVERWLVAERFTLNGSRIVWPDLDARRIEHVTASAGFRPERQPSDRADIFR
                     ITLEPNQTITYAVELASDRPTRLLLWKPVEYEQKQRNKQLFNGIMLGITGCLAIFLTV
                     IFAANHAAIFPSAALVAWCILALLCVDFGFWHKLFQMRAEDSAQYRAVAEASLAASLV
                     IFLFTFLRLRFSNAFTRLLFGVWIAGQLGLVATAIIDPRLATTVARLSFGAIGGIGAF
                     TILYLALRRQERALALSPTWMLLLVWLFAAAAVLTGKLPAEAHVVGLLGGLVLFLVLI
                     GFTVTQFAFRPHEVSFLAGVAPGGGGRSLAIDGAGAAVFEWSARRDDIKVDASIEEAL
                     GLKSGDLSVKVEDFVRYMHSADRERFEVGIQSIKDKAGGALRLDFRMRNTDSSYRWFE
                     VEATSVPTTDRRQLRCIGLIRDITDQRAAQERLLHDSVHDSLTGQPNRALFLDRLAVA
                     IQRARNDAALRPVLVFIDLDKFKSVNSSFGMTVGDSLLLTIARRLSRHLGPEDTLARV
                     GGDQFAILMLGQQDPRELAMLAERIRRALRAPIKIAGQEIVLTGSLGIAIYDGKQTSH
                     MELLREAELAMYRAKRAGADRIEIFKPAMRSERDDRVQIESDLRRAVENRELSVLYQP
                     IISLSTEELRGFEALVRWEHPRLGLLNPVDFVPVAEETDLIVRLGSFVLSRAVADIAA
                     WQDQLDRPEAPLFVSVNVSSRQLIHQDLIVEVKNLINRAGIDPACLKLEITESLVMDN
                     PEQATMVLDQLKAIGVGVALLACLPATLPDRPHQDRPLAGVGRRRGRGRRQDRALDRR
                     SGTRTRQAGCRRGGRDAGGCEFPALHRVRVRAGLPVPCTDARARGAQLLEAHPQSRQT
                     HAPARHGAGYGEEKIGGRRGST"
     gene            complement(2981..3613)
                     /locus_tag="PK264_04330"
     CDS             complement(2981..3613)
                     /locus_tag="PK264_04330"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD75154.1"
                     /translation="MAFLRHVSGLEGGAVLRGPGVMLRLPHSSDYQEWAALRLESRPN
                     LEPVEPLWADDELSRGAFRRRLAHYARDVRDGTGLAVLIFKDGGPLLGGITLSNIRRG
                     VSQTGSVGYWLGTRHWRHGHMSAALGTFVTYCFEELRLHRIEAACLPTNVASIRVLER
                     TQFRHEGLARSYLKIAGQWQDHLTFGLVADDWRQSCERAGASSRRAEGVG"
     gene            complement(3670..4914)
                     /locus_tag="PK264_04335"
     CDS             complement(3670..4914)
                     /locus_tag="PK264_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD75155.1"
                     /translation="MRVVTERMPRVETVALGVWVAAGSRHERPGEHGISHLLEHMAFK
                     GTTRRSARAIAEEIEQVGGDLNAATSIEMTSYYARILKGDVRLALDILADILLDPRLD
                     PGELAREQEVILQEIAGARDSPDDVAYDLAQEAAFAGQALGRPILGTPKSVSGTTAED
                     LRAFLKRWYTPERMVIGAAGQVDHAAVVRHAEALFGGLDRKSSTKRSKARWVGGKRAS
                     PRSFEQSHLIVGFEGPSYRDRDFFAAQVLSALLGGGMSSRLFQEVRERRGLAYSIYST
                     CWGLDDTGMLSVHAATGPSSMSELIDVVGEEMQKVASERAPPSEVQRAKAQLKAGLLM
                     SLESPGARAEQIARQIIVHDRVIETEELVDRVDAVTPNGVRSAAERLLAKGEPSIALV
                     GVGRRGRSLGARVAEKLGAVMA"
     assembly_gap    5099..5266
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5267..6344)
                     /gene="thrC"
                     /locus_tag="PK264_04340"
     CDS             complement(<5267..6344)
                     /gene="thrC"
                     /locus_tag="PK264_04340"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRD75156.1"
                     /translation="MRYVSTRGAAPDLGFEDVLLTGLARDGGLYVPREWPAFTAQDFT
                     AMAGLSYEECAARILTPFVAPDMAPAELSSLIHDAYGSFRHQAIAPLVQLGPRDWLLE
                     LFHGPTLAFKDIAMQVLARLMDRTLKRRGARATIVGATSGDTGGAAIDAFRGRDAIDV
                     FILFPHGRVSDVQRRQMTTPVEANVHAIAIDGTFDDCQAIVKALFNDLAFRDSVSLAG
                     VNSINWARILAQIVYFVTSAVALGAPGRRVAFTVPTGNFGDIFAGYGASRLGLPIERL
                     VIATNANDILARTCATGRYETRDVIATSSPSMDIQISSNFERLVFDLAGRQQGRVRAA
                     MQELAAKGSFSLTGDEHRELGRLFS"
     gene            complement(6480..7301)
                     /locus_tag="PK264_04345"
     CDS             complement(6480..7301)
                     /locus_tag="PK264_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HRD75157.1"
                     /translation="MGGSVPDGHQRGIIRQLREERILLLTVLTLGALAVLLALGTWQL
                     RRKAWKEAVIAAVAARASAPPLSGEAIAHRTCRPLSEVGLEVSCEFIPVRLIGRFDHA
                     RERHVYTSQATRSDGSGGPGYWIMTPLLLADGRTYIVNRGFVPNRMKDPASRGGGNAP
                     GETEVIGLLRSSEPRTMFMASNDPGKNVWFLRNVGELYPDLAANSGGPENKSVFIDQI
                     SAGRGGQLPVPTGGDISISNRHLEYALTWFGTAAALLGTFIAFLVSRLGGRKSST"
     gene            complement(7308..7643)
                     /locus_tag="PK264_04350"
     CDS             complement(7308..7643)
                     /locus_tag="PK264_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRD75158.1"
                     /translation="MSAGGGGGSLFAGPLSLRIREACPSCRLDLKFVDPGDGPAVFAI
                     MLLGFLVLGGALILEFKVGPPLWVQVLVWGIVTPALAFLLLRALKGLLIAQQWKYKAA
                     EARTIDKAE"
     gene            complement(7713..8024)
                     /locus_tag="PK264_04355"
     CDS             complement(7713..8024)
                     /locus_tag="PK264_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75159.1"
                     /translation="MRYSRPMPAIMALAAASALALPADAEAQSRRGYGPSSGSTVYVC
                     SRYGKGCVSGPVRPGRWGLEVRLPGGNWIDCKRDCRQTLREESIDFFETLNERSPSFR
                     R"
     gene            complement(8158..9069)
                     /locus_tag="PK264_04360"
     CDS             complement(8158..9069)
                     /locus_tag="PK264_04360"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRD75160.1"
                     /translation="MRHVAVLMGGWSAERQVSLSSGRACAAALEEAGYEVTSIDVDRG
                     IAERLAALKPDACFNALHGRWGEDGAIQGLLETLGIAYTHSGVLASALAMNKVLTKRV
                     LGAAGMPLAAEAIVDRDAALARHAMEPPYVAKPIAEGSSVGVVIVKAGANRPPQSIAA
                     IPMLEGQILVERYIPGQELTCGVIGGTPTDVIDIVPSAKLEFYDYEAKYAPGGSVHVL
                     PAKIPEAVRDKVRRLARLAHVTLGCRGVSRSDFRYDGASGDLVFLEINTQPGMTGTSL
                     VPELGAHGGMSFPELVSWIVEDASTGR"
     gene            complement(9132..10094)
                     /gene="murB"
                     /locus_tag="PK264_04365"
     CDS             complement(9132..10094)
                     /gene="murB"
                     /locus_tag="PK264_04365"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRD75161.1"
                     /translation="MSFADRTAELKALLPELKGRITANASLADLTWFRVGGPAEILLS
                     PADEADLAYALARLPSEMPVTVIGLGSNLLVRDGGIPGIVIRLGRGFGDVKAEAGHRV
                     RTGTAVPDVKVARTAAEAGIMDLAFYRGIPGSIGGALRMNAGAHGAETKDVLIEARAV
                     DRRGVVHALSNAAMGFTYRHCGAAKDLIFTEALYQGKPGEPREILAQMDEVAAYREAN
                     QPIKDRTGGSTFKNPPGNSAWKLIDAAGCRGLRVGGAKVSEMHCNFLINDKGASGEDI
                     ERLGETVRARVKATSGIELEWEIIRLGLPRDGRPTGPALAEKGP"
     gene            complement(10091..11542)
                     /gene="murC"
                     /locus_tag="PK264_04370"
     CDS             complement(10091..11542)
                     /gene="murC"
                     /locus_tag="PK264_04370"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRD75162.1"
                     /translation="MQMPGALGTFHIIGIGGIGMSAIAEILMAKGYIVQGSDQKDSAN
                     VRRLRDKGMRVFVGHDAINLVGARYVVISTAVKTGNPELEAARQKGLVIIRRAEMLAE
                     LMRLYATISVTGTHGKTTTTSLISHVFSEAGLDPTVITGGIINSWGSNARLGQGRWMI
                     VEADESDGTFVRIPTEIGVVTNIDPEHLDYFKTVERMHQEFEAFYRNIPFYGLAVTCI
                     DHPTVAAMVRRLDLRREGRRLLTYGAAPDADLVLENVRFEGRRTLFDARLGERVRGGA
                     RRLANRSVPVPGHHNALNALAAIAVAAEAGVPDDVLGRAIADFSGVKRRFQPTGTWNG
                     VEIFDDYGHHPVEIAAVLKAAKAGAKGQVIAVVEPHRYSRVADLFGEFCACFGDADQV
                     IVAPLYSAGEAPIEGVGQRSLAEGIRKTGHRAVAALDSDKEIAPLLARMAKPGDMVMF
                     LGAGNSTEWAHAMPQRLIEVPAVGGHRGKGAGA"
     gene            complement(11585..12709)
                     /gene="murG"
                     /locus_tag="PK264_04375"
     CDS             complement(11585..12709)
                     /gene="murG"
                     /locus_tag="PK264_04375"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRD75163.1"
                     /translation="MSGKLVLLAAGGTAGHLFPAFALSEELGRRGHTIDLITDERGDR
                     YGTGFPAREVHQVPAATPSGRSPVAAAKAAITLSRGIAAAYSLIGRLAPAAVIGFGGY
                     PTVPPLLAARMRRIPTAIHEQNAVLGRANRMLSKRVSAIALSFEETKLIEGTAVTRAH
                     YTGNPVRTTVIDWAQRAYRAAGAAERFDLLVFGGSQGARYFSDAVPPALALLPAGTRA
                     RLVVTQQCRPEDIDRVRAAYAASGVTADLASFFKDLPERMAASQLVIARAGASTIAEL
                     AVMGRPSILVPLPHALDNDQLQNARQLAGLGGAWCIEQKDLTPDRLAAEISRLMGEPS
                     LLPAAAAAARSAGRADAVLRLADLVEEIMQVRAPAKAARA"
     gene            complement(12706..13890)
                     /locus_tag="PK264_04380"
     CDS             complement(12706..13890)
                     /locus_tag="PK264_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRD75164.1"
                     /translation="MRLSRTDDSLLAEWWLTVDRLLIAAAFALMAAGVVLSLAASPAV
                     AIKKGLEPFHFVERHVVWAVIAAGVMLWVSLLSARQARRLALLIFLGALATMIFVLVM
                     GPDINGARRWLRIAGLSVQPSELAKPAFVVLAAWALNEATERRDVPAIGVGVVLYGAF
                     AVLLALQPDVGQTLLVTAVFGALFLLSGLSLKWVGALAGAGVAGLAVAYQAFPHVRRR
                     IDRFIDPQSGETYQTDRALQSFLDGGFFGRGPGEGTIKAVLPDAHTDFIMAVVAEEYG
                     ILACLGLLLVFAFIVFRSIIGLARAPDGFVRLAVTGLALLIGLQALINMSVSVGLIPP
                     KGMTLPFVSSGGSSGIASALTMGLLLALRRWPADARRVKLPEFGLGSPLAAGTAGAKE
                     RS"
     gene            complement(13887..15340)
                     /gene="murD"
                     /locus_tag="PK264_04385"
                     /pseudo
     CDS             complement(13887..15340)
                     /gene="murD"
                     /locus_tag="PK264_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
     assembly_gap    15408..15605
                     /estimated_length=198
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15606..>15974)
                     /locus_tag="PK264_04390"
     CDS             complement(<15606..>15974)
                     /locus_tag="PK264_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75165.1"
                     /translation="AACGHAGGLARAGPGRIWVADTGALFEIEVAVPSAPGIGRVMRT
                     IPLGGLVRGSFAAGGTGSILLGRYDKQPGARLWRFDIAGLPATLDERVALGSIPLPIE
                     AQGAAVDAAGRLWITRSGSLY"
     assembly_gap    15975..16069
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16070..16312)
                     /locus_tag="PK264_04395"
     CDS             complement(<16070..16312)
                     /locus_tag="PK264_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75166.1"
                     /translation="MNLRRSALSGLIVLAVVLQVVATDACAEPLGTAPTYTTRVLSEP
                     PNAAAISTRIWAPGLDEGYVPQGLTFAEGAIFLGTYR"
     gene            16555..16893
                     /gene="yajC"
                     /locus_tag="PK264_04400"
     CDS             16555..16893
                     /gene="yajC"
                     /locus_tag="PK264_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRD75167.1"
                     /translation="MFVSPAFAQGAGGGSDIFGLLMPMLLILPIFYFLIIRPQQQRVK
                     QHREMVANIRRNDTVVLSGGILGKVTKVKDSDNEVEVEIADNTRVRVLRHTIAEVRAK
                     GEPVKDSTTA"
     gene            16956..18599
                     /gene="secD"
                     /locus_tag="PK264_04405"
     CDS             16956..18599
                     /gene="secD"
                     /locus_tag="PK264_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRD75168.1"
                     /translation="MLHFAPWKIWSIIGACLVSLLLAIPNFLSKATLDQLPPWLPKKQ
                     ISLGLDLRGGAHILLSMSTDEVRKAWLQTIAEDARKRLVTAKNKDGQTLGYEGRPAVV
                     GKIVQVRPARPEDGPLAITELKKMIQPLTSGSFIGVGGPPDLEITADASGLITISPTE
                     QAIQDRITAAISAAIETVRRRVDPNGTVEATIVQQGRDRILVQVPGIEDTTKLKELIG
                     ETALLTLHLLHPSISAEEAKARGSTPTGYRIYESADGLRGSYLLAEPAIRGDDIAGAQ
                     PATDSRTGEPVVSFQLKNAGARVFGQLTTDNVGKPFAIVLDGKVLSAPVIQDKILGGS
                     AQISGRFSFEEVTKLALQLRSGALPAKLTIIEERSVGPTLGADSIEAGSRAGIIGGIV
                     TMIMTIAAYGTFGIFAVIALLVNGLMIVAIMSLMGSTLTLPGIAGLVLTIGMAVDANV
                     LIYERIREELRAGRTAIAAIDAGFSRAIVTIADSQLTTLAAAIIMFWLGSGPIRGFAV
                     TLTIGIFTSVFTAVTVTRLLVSWWLKNARARQKTIEVPI"
     gene            18682..>19085
                     /locus_tag="PK264_04410"
     CDS             18682..>19085
                     /locus_tag="PK264_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRD75169.1"
                     /translation="MVPVPHFKGFDFFPHGTRIKFMRFRDICFWGSIAAMVISLALFL
                     TKGLNYGVDFKGGSMVEVQAKTGKADVAALRTKLSGIGLGDVQIQPFGKAGDVLIRIE
                     QQPGGEKAQQDAVAKVMGLIKDEYNQRQVQVVG"
     assembly_gap    19086..19095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19096..19637
                     /gene="secF"
                     /locus_tag="PK264_04415"
     CDS             <19096..19637
                     /gene="secF"
                     /locus_tag="PK264_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRD75170.1"
                     /translation="AVSSELRTTGIIAVIASILAIIMYVWFRFEWQFALGGVLALSHD
                     VLLTVGLFSLFQLEFDLSIVAALLTILGYSINDTVVVSDRIRENLRKYKRMALTELLD
                     LSINETLSRTVLTGMTTLAVLVCLWIWGGPVLRNFTFAMLFGVLVGTYSSIFIAAPLL
                     DYLGVRRETVVGTEKAKRA"
     gene            19654..20052
                     /locus_tag="PK264_04420"
     CDS             19654..20052
                     /locus_tag="PK264_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HRD75171.1"
                     /translation="MNSDGQAAAAGSGRYPGQAPIDAYGNGGFRFAGMSHRGAILCLP
                     SAILVWRPSAVDDIGEPDLAAVLAEPRAVKHLLIGTGLRFALPPRAILRRLADAGIGA
                     EPMDTGAACRTYNILLSERRPVAAALLPVA"
     gene            20091..>21022
                     /locus_tag="PK264_04425"
     CDS             20091..>21022
                     /locus_tag="PK264_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRD75172.1"
                     /translation="MTRNLRPETIAAQACGIVDEATGAIVLPIGVATTFIRDPDNQYR
                     KGYSYGRADNTTVRLAEDVLTRLEGGALSVVLGSGMSAAIASFLALDRPAHLIAPKIM
                     YWGLRAWLLDDAPGYGITTTFVDTADLDAVRAAIRPGQTRMIWAETPSNPMWGIADIA
                     ALAEIAHKAGAILGVDSTAATPVLSNPIALGADVVMHSATKYLNGHSDVIAGALVLAR
                     SGPYADRVLRVRGKLGLILGPFEASLLVRGMRTLHVRVRHQTASAMKIAEHFAADRRV
                     GAVLYPGLPGHPGHAVAARQMSGGFGGMISIRIAA"
     assembly_gap    21023..21099
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21467..21811
                     /locus_tag="PK264_04430"
     CDS             21467..21811
                     /locus_tag="PK264_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HRD75173.1"
                     /translation="MTTSSRRPADCRDLADVRVEIDRCDKALVDLIGERFGYVERAWQ
                     IKLGLKQEANVPWRNQQVIDRVRARATERGVPPDLCEALWRQMIGWFIQYEEEKLRHE
                     IEGSGKGKDATG"
     gene            21816..22721
                     /locus_tag="PK264_04435"
     CDS             21816..22721
                     /locus_tag="PK264_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRD75174.1"
                     /translation="MAITRRAAISGGIGLAFAGAGPAVAQPRARKPRLPPGRNPGGVP
                     VAVIDGGIDYTRADIAGRLARDGEGDLVGWDLDDNDNRPFERAWPGGGEPQGTLLATI
                     MLAEAGASTVVPVRRRDRDGLSIARALSFVSQTPARFAVLPFASDDPDFWAKFREAAT
                     HLSGVMVIVPAAMQGGASRDLAATPSYPAAFGLPNVLSVTAVDTAGMLLHGAPRGAAS
                     IDLAVPAHDTVGYGRDGEKVPIPGTLGAVMRAAGMAARLQEREPHLRGAALKSRLLAD
                     ARPLPDPTIARAGWIADARRRYWLE"
     gene            complement(22733..23122)
                     /locus_tag="PK264_04440"
     CDS             complement(22733..23122)
                     /locus_tag="PK264_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75175.1"
                     /translation="MPSARRMMRAAAMAAALALIGSGGAMLPLSKDAVAQARKKGNDE
                     RPEQFPAGPHRDDAFYFCTACHGFAIVASQGMSRERWDESLTWMVDRHGMPKLEGAER
                     EKILDYLGVAFPERKAPGGWKNPFAPQ"
     gene            complement(23128..>23493)
                     /locus_tag="PK264_04445"
     CDS             complement(23128..>23493)
                     /locus_tag="PK264_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin containing oxidoreductase"
                     /protein_id="HRD75176.1"
                     /translation="ILESMPVRGIITNPMNGTKLAAGTRSLALRGAAWAGDRDVARVD
                     VSTDFGASWSRASLANSRNRFDWRRWTAKVALPSDGYYEVWVRATDTRGVMQPHIAGN
                     WNPQGYGGNPMHRIAVLVG"
     assembly_gap    23494..23531
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23532..24524)
                     /locus_tag="PK264_04450"
     CDS             complement(<23532..24524)
                     /locus_tag="PK264_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD75177.1"
                     /translation="MKDGKPSPKLGKRERSVDELYTDDADRADAVIFGRKTDVSRRGF
                     LGGAGLGAMTAAVGGTIVYQATMPAGLVPAALAQDPKPAPKPAAPAPAAAAPTAPAAP
                     KRFDFPGKDPGLTLLGDKPLVAETPEHLLDDDTTPVSKFFIRNNGTPPKVAGDVDAWK
                     LKIDGEVATPLELALGDIKKRFKAVTLRSVLECGGNGRSFFTPQARGNQWTNGGAGCA
                     EWTGVLLSDVLKAAGLKPSAVYTGHYGADVHLSGDAKRDVLSRGVPVAKAMQPQSLLV
                     WAMNGKPLEAIHGAPLRLVIPGWPGSVSHKWLTRITLRDKVHDGAGMTGTSYRVA"
     gene            25048..>25317
                     /locus_tag="PK264_04455"
     CDS             25048..>25317
                     /locus_tag="PK264_04455"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HRD75178.1"
                     /translation="MTVADQQTEWYLARDGKPYGPMSASEMAKVVDLGYLRESDLVWR
                     PGFAEWLPAFQVFPPQPDPVPEPASAAPAPAPPPAGSAFRPADQPV"
BASE COUNT         4052 a         8519 c         8203 g         3957 t
ORIGIN      
        1 catgtgctgc cgcgccgtcc tccgattttt tcttctccgt accccgcacc atgccgcgcc
       61 ggcgcatgcg tttgtcggct ttgcgggtga gcctcaagta attgagcacc tcgcgctcgg
      121 gcatcggtgc acggaacagg aagccctgcg cgaactcgca cccggtggag cgcaggaaac
      181 tcacatcctc cggcgtctcg accccctcgg cgacaacctg cttgccgagt tcgtgtgcca
      241 gaacgacgat cgagcgcacg atcttggcgc cgcccacgtc ctctgcggcg accgacacca
      301 gcgagcggtc gatcttgatg aggtcgatcg ggaagcgttg caggtaggca agcgaggagg
      361 gcgacgccga cgccgatggc cttcagctga tcgagcacca tcgtcgcctg ctcgggattg
      421 tccatcacga gcgattcggt gatttcgagt ttcaggcacg ccggatcgat gccggcccga
      481 ttgatgaggt tcttcacctc gacgatcagg tcctggtgta tgagctgccg gctcgacacg
      541 ttcaccgaga cgaacagcgg agcctccgga cggtccagct ggtcctgcca ggcggcaatg
      601 tccgcaacgg cgcgactcag cacgaacgag ccgagacgca cgatgaggtc cgtctcctcg
      661 gcaaccggaa cgaagtcgac cggattgagc aggccgaggc gcggatgctc ccagcggacg
      721 agcgcctcga agccgcgcag ctcctcagtg gagagcgaga tgatcggctg gtagagcacg
      781 ctcagctcgc ggttctcgac ggctcgcctg aggtcgcttt cgatctgtac gcggtcgtcc
      841 cgctccgagc gcatcgcggg cttgaagatc tcgatacggt cggcgccggc ccgcttggcg
      901 cgatacatgg cgagctcggc ctcgcgcagg agctccatat ggctcgtctg cttgccgtcg
      961 tagatggcaa tgccgagaga gccggtgagc acaatctcct gacccgcgat cttgatcgga
     1021 gcgcgcagcg cacgccggat gcgctcggcc agcatggcga gctcgcgcgg gtcctgctgg
     1081 ccgagcatga gaatggcgaa ctggtcgccg ccgacgcggg cgagcgtgtc ctcgggtccg
     1141 aggtgacgcg agagccggcg tgcgatcgtc agcaacaggc tgtcgccgac cgtcatgccg
     1201 aaggagctgt tcacgctctt gaacttgtcg aggtcgatga agacgagcac ggggcgaagc
     1261 gccgcgtcgt tgcgggcgcg ctggatggcg acggcgaggc gatcgaggaa cagggcgcgg
     1321 ttcggctggc cggtcaggct gtcatgcacc gaatcgtgca gcagccgctc ctgcgctgcc
     1381 cgctggtccg taatgtccct gatgaggccg atgcatcgca gttgccgccg gtcggtcgtc
     1441 ggcacgctcg tggcctcgac ctcgaaccag cggtaggagc tgtcggtgtt tcgcatgcgg
     1501 aagtcgagcc tcagcgcccc gcccgccttg tccttgatac tctggatgcc gacctcgaag
     1561 cgctcgcgat cggccgagtg catgtagcgg acgaagtcct cgaccttgac cgagagatcg
     1621 ccggacttca gcccgagagc ctcctcgatg ctggcatcga ccttgatgtc gtcgcggcgt
     1681 gcgctccatt cgaagaccgc ggcacccgcc ccgtcgatgg cgagcgagcg accgccgccg
     1741 cccggcgcca cacccgcgag gaatgacacc tcgtgcggcc ggaaggcgaa ctgcgtgacc
     1801 gtgaagccga tcagcaccag gaagaggacg agcccgccga gcaggccgac gacgtgggcc
     1861 tcggccggca gcttgccggt gagcacggct gcggccgcga atagccagac gagcagcagc
     1921 atccaggttg gcgacagtgc cagcgcgcgc tcctgccggc gcagtgccag gtagaggatg
     1981 gtgaacgcgc cgatgccgcc gatggctccg aacgacaagc gcgcgaccgt cgtcgcgagg
     2041 cgcggatcga tgatggccgt ggcgaccagg ccgagttggc cggcgatcca cacaccgaac
     2101 aggaggcgtg tgaacgcgtt cgagaagcgc agcctcagaa acgtgaagag gaatatgacg
     2161 aggctcgccg cgagcgaggc ttccgccacc gcccgatact gtgcgctgtc ctcggcgcgc
     2221 atctggaaga gcttgtgcca gaagccgaag tcgacgcaga gcagcgccag gatgcaccag
     2281 gcgacgagcg ccgcggatgg gaagatcgcc gcgtggttgg ccgcgaagat gacggtgagg
     2341 aagatcgcga ggcagcccgt gatgccgagc atgatgccgt tgaagagctg cttgttgcgc
     2401 tgcttctgct cgtactcgac cggcttccag aggaggagcc gcgttggccg gtctgaggcg
     2461 agctcgacgg cataggtgat cgtctgattg ggctcgagcg tgatgcggaa gatgtcggcc
     2521 cggtcgctcg gttggcgttc cggacggaag cctgcagatg ccgtcacgtg ctcgatgcgg
     2581 cgcgcatcga gatcaggcca gacgatgcgc gagccattga gggtgaagcg ctcagcgacc
     2641 agccagcgct cgacgggctt gtccgtggtg ttgcggatag cgaagacaaa ccagctggtg
     2701 ctctgcgtag ttgcggcgac ggtcatgcgc ccccccgtgc cgtcggtgcc gggcgccatg
     2761 tcgacgtgca gcttgtcgcc gccggcctcg atggaacggc cgatcgccgt gacgtcgatg
     2821 cggtcgagcg tcggatcaat cgcatgggct tggatcggct gcgccgacgc ctgagccgtc
     2881 gagagcgcaa gaagcgccac gaccgcgacg acgagcaaag cgagccgccc tccccgggag
     2941 tagatcccgc ggtctactga aaggcgatcg gaccgcgctg tcatccgacg ccctccgctc
     3001 gccgagagga cgcgcccgca cgctcgcagg attgccgcca gtcgtcggca acgagcccga
     3061 aggtcaggtg gtcctgccat tggccggcga tcttcaggta gctgcgggcg agcccttcat
     3121 gccggaactg cgtgcgttcg aggacgcgga tggacgcgac atttgtcggc aggcaggccg
     3181 cctcgatgcg gtgcagccga agctcctcga aacagtaggt gacgaaggtc ccaagcgccg
     3241 ccgacatgtg cccgtgccgc cagtgccgcg tgccgagcca gtagccgacc gagcccgtct
     3301 ggctcactcc gcggcggatg ttgctcaagg tgatgccgcc gagaagcggg ccaccgtcct
     3361 tgaagatgag caccgcgaga ccggtgccgt cacggacgtc ccgcgcgtag tgggcgagcc
     3421 ggcggcggaa ggccccgcgc gagagctcgt catccgccca gagcggctcg accggctcca
     3481 ggttcgggcg gctttcgagg cggagtgccg cccactcctg atagtcgctg ctgtgcggca
     3541 ggcgcagcat cacgccggga ccgcgcagaa cagcgccgcc ctcgaggccc gacacgtgcc
     3601 gcaggaatgc cattcccctc gctctcctca ccgtcgatcc ggcggcggac catagagtgt
     3661 tactctggat caagccatta cggccccgag cttttccgca actctagcgc cgagtgagcg
     3721 gccgcgtcgc ccgacgccga cgagcgcgat cgacggctcg cccttggcaa gcagtcgttc
     3781 tgcggctgag cgaacaccgt tcggtgtcac ggcgtcgacc cggtcgacca gttcctccgt
     3841 ctcgatcacg cgatcatgca cgataatctg tcgcgcgatc tgctccgccc gcgcgcccgg
     3901 gctctcgagg ctcatgagga gccccgcctt caactgggcc tttgcgcgtt gcacctcgct
     3961 cgggggcgcg cgctcgcttg ccaccttctg catttcctcg ccgaccacat cgatcaactc
     4021 gctcatggac gacggaccgg ttgcggcatg gacggacagc atgccagtgt catcgaggcc
     4081 ccagcaggtc gagtagatcg aataggcgag gccgcgccgc tcgcgcacct cctggaagag
     4141 gcgcgacgac atccccccgc cgagaagtgc ggagaggacc tgcgccgcga agaagtcccg
     4201 gtcacgatat gacggcccct cgaatcccac gatcagatgg ctctgctcga agctgcgagg
     4261 cgacgcgcgc ttgcccccga cccagcgcgc cttcgatcgc ttcgtgctcg acttgcgatc
     4321 gagcccgccg aatagggcct cagcatgacg gacgactgcg gcatggtcga cctggccggc
     4381 ggcgccgatc accatgcgct cgggcgtgta ccagcgtttc aggaaggcgc gcaggtcctc
     4441 ggcggtcgta cccgagacgc ttttcggcgt gccgaggatc ggccggccca gcgcctggcc
     4501 tgcgaaagcc gcctcctgcg cgaggtcgta ggcgacgtcg tcggggctgt cgcgggcgcc
     4561 ggcgatctcc tggaggatca cctcctgctc acgggcgagc tcgcccggat cgagcctggg
     4621 atcgagcaag atgtcggcca ggatgtcgag cgcgaggcgc acgtcaccct tcaggatgcg
     4681 ggcgtagtaa gaggtcatct cgatgctggt cgcagcgttg agatcgccgc cgacctgctc
     4741 gatctcctcg gcgatggcgc gggccgaccg ccgcgtcgtc cccttgaagg ccatgtgctc
     4801 gagcaggtgg ctgataccgt gctcgcccgg ccgctcgtga cgcgagccgg cagccaccca
     4861 cacgccgagc gccaccgtct cgacgcgcgg catgcgctcg gtcacgacac gcatcccatt
     4921 ggaaaggcgg gagatttgta cgctcatgtc ttggcctttc cggcgcgtgt gcgcccggca
     4981 atgaaccgtg cgacctcggt cgcgtcatgt ctgaggagtg tcatgcgctc cttggccgag
     5041 cgctgcgcct tgacggacgc cggctcggca ggtggcgcgc cggcgtggct cgaggctcnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctga aaagagtctt
     5281 ccgagctccc tgtgctcgtc gccggtgagg ctgaagcttc ccttggcggc gagctcctgc
     5341 atggctgccc tgacgcggcc ctgctggcga ccggcaagat cgaacaccag ccgttcgaag
     5401 ttcgacgaga tctggatgtc catgctcggg ctcgacgtcg cgatgacgtc ccgcgtctca
     5461 tagcggccgg tcgcacaggt gcgggcaagg atgtcgttgg cgttggtcgc gatcacgagg
     5521 cgctcgatcg gcaggccgag gcggctcgcc ccgtagcccg cgaagatgtc gccgaagttc
     5581 cccgtcggca ccgtgaaggc gacccgacga cccggcgcgc cgagcgccac cgccgaggtc
     5641 acgaaataga cgatctgggc gagaatgcgc gcccagttga tcgagttcac accggcgagc
     5701 gagactgaat cacggaacgc aagatcgttg aacagcgcct tcacgatcgc ctggcagtcg
     5761 tcgaaggtgc cgtcgatggc gatggcatgc acgtttgcct cgaccggcgt cgtcatctgc
     5821 cgacgctgca cgtccgagac gcggccatgc gggaagagga tgaacacgtc gatggcatcg
     5881 cgaccgcgga aggcatcgat cgcagctccg ccggtatcgc cggaggtggc gccgacgatg
     5941 gtcgcgcgcg cgccgcggcg cttcaaagtg cgatccatga ggcgcgctag cacctgcatg
     6001 gcgatgtcct tgaaggcgag cgtcggtccg tggaagagct cgagcagcca gtcgcggggg
     6061 ccgagctgca cgaggggcgc aatcgcctga tgtcgaaagc tgccgtaggc gtcatggatg
     6121 aggctcgaga gctcggccgg cgccatgtcg ggcgcaacga acggcgtgag aatccgcgcc
     6181 gcgcactcct cgtaggagag gccagccatg gcagtgaagt cctgggccgt gaatgccggc
     6241 cactcgcgag ggacatagag cccgccgtcg cgggcgagcc cggtcagcag cacatcctcg
     6301 aagccaaggt ccggggccgc accccgcgtg ctcacgtatc tcacgtcgcg actcgcttaa
     6361 cgcatcattg cgcggggacg ttaaacttcg cctccgggcc tcgcaagcag cacctcggtc
     6421 acgcgcacag ctgcagttcc gccatgccgt gaacgttgct tacgtcacct tgccgtaggc
     6481 taagtcgagg acttgcggcc tccaagacga gacacaagaa aagcgatgaa ggttccaagc
     6541 aaagcggcgg ccgtgccaaa ccaggtgagc gcatattcga gatggcgatt ggagatgctg
     6601 atgtctccgc cggttgggac cggcagttgc cccccgcggc cggccgagat ctggtcaatg
     6661 aacacggatt tattctcagg cccgccagag ttggctgcca aatctggata gagctcgccg
     6721 acgtttcgta agaaccagac gttcttgccg ggatcattgc tcgccatgaa catggtgcgc
     6781 ggttctgacg agcgcaacag cccgattacc tcggtttcac ccggtgcatt gcccccgcca
     6841 cgggatgccg gatccttcat gcgattgggc acgaatccac gattgacgat ataggtccgg
     6901 ccgtccgcga gcagcagcgg cgtcatgatc cagtatcctg ggccgccact gccatcgctg
     6961 cgggtcgcct gcgaggtgta aacgtgacgc tcgcgtgcgt gatcgaaacg gccgatgagc
     7021 cggaccggga tgaactcgca cgacacttcg aggccgacct cgctcagcgg ccggcacgta
     7081 cgatgggcga tcgcttctcc actcaatggc ggagccgacg cgcgcgcggc taccgccgcg
     7141 atcacggcct ccttccaggc cttgcggcgg agttgccagg tgccgagcgc caacagcacg
     7201 gcaagtgccc ccagcgtcag caccgtgagg agcaggatgc gctcctcgcg aagctgccgg
     7261 atgatcccgc gctgatggcc gtcgggcacg ctgccaccca tgctcgccta ctcggccttg
     7321 tcgatcgtgc gggcctcggc ggccttgtac ttccattgct gcgcgatcag cagacccttg
     7381 agggctctga gcagcaggaa ggcgagcgca ggcgtgacga tgccccaaac cagcacctga
     7441 acccagagcg gcggtccgac tttgaactcg aggatcaagg cgccgccgag caccagaaag
     7501 ccgagcagca tgatcgcgaa cacggccggt ccgtcgcccg gatcgacgaa cttgaggtcg
     7561 aggcggcacg acgggcacgc ctcgcgaatg cgcagcgaga gcggacctgc gaagaggctc
     7621 cccccccccc cccccgcgga catcgcccac cgatcccgct ggcgatcacg gacggggagg
     7681 gcgtctcgct cgtcatatcg cgctctgcca cctcagcgcc ggaagctagg cgagcgctcg
     7741 ttcagcgtct cgaagaaatc gatcgattcc tcccgcagcg tctgccggca gtcgcgcttg
     7801 caatcgatcc agttgccgcc gggaagcctg acctcgaggc cccaccgccc aggccggaca
     7861 ggccccgaga cgcagccctt gccgtagcgg ctgcacacgt acacggtcga accactgctc
     7921 gggccgtagc cgcggcggga ctgcgcctcg gcatcagctg gaagcgcgag cgcggacgca
     7981 gcggcgagcg ccatgatcgc aggcatcgga cgggagtatc gcatgtcgca cctccgttgg
     8041 gacttcgtga agctggtagg ccagcttgcg gcgaaaggcc agtgacttgg tcacagaccg
     8101 gcccacacat gcgcgtgatc cagactgcct tgtctcgtcg ccggcatgac caccggctca
     8161 gcgcccggtc gatgcgtcct cgacgatcca gctgacgagc tcgggaaagc tcattccacc
     8221 gtgggcgccg agctcgggga cgagcgatgt cccggtcatg ccgggctgtg tgttgatctc
     8281 gaggaagacg agatcgccgc tcgcgccgtc gtagcggaaa tcagagcgcg agacgccgcg
     8341 gcagccgagc gtcacatgcg cgaggcgcgc gagacggcgg accttgtcgc gcaccgcctc
     8401 cgggatcttg gccggcagga catgcaccga gccccccggc gcatacttcg cctcgtagtc
     8461 gtagaactcg agcttggccg acggcacgat atcgatgacg tcggtcggag tacccccgat
     8521 cacgccgcag gtgagctcct gccctgggat gtagcgctcg accaggatct gcccttcgag
     8581 catcgggatc gcggcgatcg actgcggcgg gcggttggcg ccggccttca cgatcacgac
     8641 gccgacgctg gagccctcgg cgataggctt tgcgacgtac ggcggctcca tggcatggcg
     8701 tgcgagcgcc gcgtcccggt cgacgatcgc ctcggcggcg agcggcatgc cggcagcgcc
     8761 gagcacgcgc ttcgtcagca ccttgttcat ggcgagcgcg gaggcgagca cgccggagtg
     8821 cgtataggca atccccagcg tctcgagcag gccctggatc gcgccatcct cgccccagcg
     8881 gccgtgcagc gcattgaagc atgcgtcggg cttcagtgct gcgaggcgct ctgcaatgcc
     8941 gcggtcgaca tcgatggagg tgacctcgta gccggcctcc tccagggccg cggcgcaggc
     9001 ccgaccggac gataggctca cctgccgctc ggccgaccag ccgcccatca gcacggcgac
     9061 atgccgcatc ttacgttctt gcgggacgaa gccgcggagc gccccggcag tcggtgcgag
     9121 atcactcatg gtcacggtcc tttctcggcg agcgccggtc cggtcggccg gccatcgcgc
     9181 gggagcccca ggcgaatgat ctcccactcg agctcgatgc cgctcgtcgc cttgacgcga
     9241 gctcgaacgg tctcgccgag gcgctcgatg tcctcgccgc tcgcaccctt gtcgttgatg
     9301 aggaagttgc agtgcatctc cgagaccttg gcgccaccga cccgcaagcc gcggcagccg
     9361 gccgcgtcga tgagcttcca ggcgctgttc ccgggcgggt ttttgaaggt cgagccgccg
     9421 gtgcggtcct tgatcggctg gttcgcctcg cgataggcgg cgacctcgtc catctgcgcg
     9481 aggatctcgc gcggctcgcc gggcttcccc tggtagagcg cttcagtgaa gatcaggtcc
     9541 ttcgcggcgc cgcaatggcg ataggtgaag cccatcgccg cattcgagag ggcgtgcacg
     9601 accccgcgcc ggtcgacggc gcgcgcctcg atcagcacat ccttcgtttc cgcgccgtgt
     9661 gccccggcgt tcatgcgcag cgccccgccg atcgagcccg gaatgccgcg atagaacgcg
     9721 agatccatga tgccggcttc ggccgccgtg cgcgcgacct tcacgtcggg caccgctgtt
     9781 ccggtccgca cgcgatgtcc cgcctcggcc ttcacatcgc caaagccgcg accgaggcga
     9841 atgacgatgc cgggaatgcc gccgtcgcgc acgagcaagt tggatccgag cccgatcacg
     9901 gtgaccggca tctccgaggg caggcgcgcc aaggcgtagg cgagatcggc ttcgtcggcg
     9961 ggcgatagca ggatctcggc cggaccgccc acgcggaacc aggtgaggtc ggcgagcgag
    10021 gcattggccg tgatgcgccc cttgagctcc gggaggagcg ccttcagctc ggcggtgcga
    10081 tcggcgaagc tcacgcgcca gctcctttgc cccgatgacc gccgacggct ggcacctcga
    10141 tgaggcgctg cggcatggca tgcgcccatt ccgtgctgtt gccggcgccg aggaacatca
    10201 ccatgtcgcc gggctttgcc atccgggcga ggagaggcgc gatctccttg tcgctgtcga
    10261 gggccgcgac cgcgcgatgc cccgtcttcc ggatgccttc ggcgagcgag cgctgcccga
    10321 caccctcgat cggcgcttca cctgccgagt agagtggcgc gacgatcacc tggtcggcat
    10381 cgccgaagca ggcgcaaaac tcaccgaaca agtcggcgac gcgcgagtag cgatgcggct
    10441 ccacgaccgc gatgacctgg cccttggcac ccgctttcgc cgccttaagt acggccgcga
    10501 tctcgaccgg gtggtggccg tagtcgtcga agatctcgac gccgttccag gtgcctgtcg
    10561 gctggaagcg gcgtttcacg cccgagaaat ccgcgatcgc ccggccgagc acgtcgtccg
    10621 ggacgccagc ctctgccgca accgcgatcg ccgccagcgc gttgagtgcg ttgtggtggc
    10681 ccggaaccgg cacggaccgg ttcgcaagcc gccgcgcccc gccccggaca cgctcgccga
    10741 gacgggcgtc gaagagagtc cgccgtccct cgaagcgcac gttctcgagc acgagatcgg
    10801 catccggcgc cgccccatag gtgagcagcc ggcgcccttc gcgtctgaga tccagccggc
    10861 gtaccatggc tgcgaccgtc ggatgatcga tgcaggtgac ggcgaggcca tagaacggga
    10921 tgttgcgata gaacgcctcg aattcctggt gcatgcgctc gaccgtcttg aaatagtcga
    10981 ggtgctcggg gtcgatgttg gtgacgacgc cgatctcggt cgggatgcgc acgaaggtgc
    11041 cgtcgctctc gtcggcttcg acgatcatcc agcggccctg cccgaggcga gcgttcgaac
    11101 cccacgaatt gatgatgccg ccggtgatga cggttgggtc gaggcccgcc tcgctgaaca
    11161 cgtgcgagat gagcgaggtc gtcgtcgtct tgccgtgcgt gcccgtgacg gagatggtgg
    11221 catagaggcg catcagctcg gccaacatct cggcgcgacg gataattacc aggcccttct
    11281 ggcgcgccgc ctcgagctcc ggattgccgg tcttcaccgc cgtcgagatc accacatagc
    11341 gcgcgccgac gaggttgatg gcatcatggc cgacaaacac gcgcatgccc ttgtcgcgga
    11401 gccgccgcac atttgcgctg tccttctggt cgctcccctg gacgatatag cccttcgcca
    11461 tcaggatctc ggcaattgca ctcatgccga tgccgccgat gccgatgatg tgaaaggttc
    11521 cgagcgcacc cggcatctgc atgctgtcga tccttcttgt cgctcaggcc tttacattgc
    11581 ccggtcaggc tcgcgccgct ttggcgggtg ccctcacttg catgatctcc tcgacgaggt
    11641 cagcgaggcg cagcaccgca tccgcccgcc cggccgaacg cgctgctgcc gcggcagccg
    11701 gcagcaacga gggctccccc atcagccggc tgatttccgc cgcgagacgg tccggggtca
    11761 gatctttctg ctcgatgcac catgcgccgc cgagcccggc aagctgcctt gcattttgta
    11821 gttgatcgtt gtcgagggcg tgcggcagcg gcaccaggat cgacgggcgc cccatcaccg
    11881 cgagctccgc aatggtcgag gcgccggccc gcgcgatcac cagctgactt gccgccatgc
    11941 gctcggggag gtccttgaaa aagctcgcga gatcggccgt aacgccgctc gcggcatagg
    12001 cggcgcgaac gcggtcgatg tcctcggggc ggcactgctg cgtgaccacg aggcgggccc
    12061 gcgtcccggc cggcaacagg gcaagggcgg gcggcacggc atcggagaaa tagcgcgcac
    12121 cctggctgcc gccgaagacc agaagatcga accgctctgc ggcccccgcg gcacgatacg
    12181 cgcgctgtgc ccagtcgatc acggtcgtgc gtaccggatt gccggtgtag tgcgctcgcg
    12241 tgacggctgt gccttcgatg agcttcgtct cctcgaagga gagggcgatg gcgctcacgc
    12301 gcttcgagag catgcgattg gcgcgaccga ggacggcatt ctgctcgtgg atggcggtcg
    12361 ggatgcgccg catgcgggca gccagcagcg gcggcacggt cggatagccg ccgaagccga
    12421 tgacggccgc cggcgcgagg cgtccgatca gcgaatacgc cgcggcaatc ccgcgcgaga
    12481 gtgtgatcgc agccttggcg gcggcgacgg gcgagcgccc ggacggagtt gccgccggca
    12541 cctggtggac ctcgcgggcg ggaaagccgg taccgtagcg gtcgccgcgc tcgtccgtga
    12601 tgaggtcgat cgtgtggccc cgccggccga gttcctcgga aagggcaaag gccgggaaca
    12661 ggtgccccgc cgtaccgccg gctgccaaca ggacgagttt gccgctcacg accgctcctt
    12721 cgctccggct gtacccgccg caagcggtga tcctagcccg aactcgggca attttacgcg
    12781 ccgagcatcg gccggccaac gtcgcaacgc gagcagcagt cccatggtca gggcgctggc
    12841 gatccccgac gagccgccgc tcgagacgaa cggcaaggtc atgcccttcg gcgggatgag
    12901 gccgacgctc acgctcatgt tgatgagcgc ctgcagcccg atgagcaacg cgagcccagt
    12961 taccgcaagg cgcacgaacc cgtcgggcgc acgcgcgagc ccgatgatcg agcggaagac
    13021 gatgaaggcg aagacgagca gcaacccgag gcaggcaagt atgccgtatt cctccgcgac
    13081 cacggccata atgaagtcgg tgtgtgcgtc cggcagcacg gccttgatgg ttccctcgcc
    13141 ggggccgcgg ccgaagaagc cgccatcgag gaatgactgc agcgcccgat ccgtctgata
    13201 ggtctcgccc gattgcggat cgatgaagcg atcgatgcgc cggcggacgt gcgggaacgc
    13261 ctgataggca acggcaagtc cggcaacccc ggctcccgcg agcgccccca cccatttgag
    13321 cgaaaggccg gagaggagga agagtgcacc gaatacggct gtgacgagca gcgtttggcc
    13381 gacatcgggc tgcaaggcaa gaagcaccgc gaaagcgcca tagagaacca cgccgacgcc
    13441 gatcgctggc acgtcgcgcc gctcggtcgc ttcgttcaga gcccaggcgg cgagcacgac
    13501 gaaggccggc ttggcaagct ctgaaggttg caccgacagg cccgcgatcc ggagccatcg
    13561 ccgcgcgccg ttgatgtccg gtcccatgac gaggacaaag atcatcgtcg cgagcgcgcc
    13621 gaggaagatc agcagcgcaa gccgccgcgc ctgccgcgcc gagaggagtg acacccacag
    13681 catgacgccc gcggcgatca ctgcccacac gacatgccgc tcgacgaaat ggaagggctc
    13741 gagacccttc ttgatggcaa cagcaggact cgccgcgagc gagagcacca cgcccgccgc
    13801 catcagcgcg aacgcggcgg cgatcagcag ccggtcgacg gtgagccacc actcggcaag
    13861 caggctgtcg tcggtgcggg acagtctcat ttccgtccgc ccccgttcgc aacgccagga
    13921 atggccgttg caagacggcg gaactcggcg ccgcggtgct cgaagttgcg gaactggtcg
    13981 taggaggcgc acgccggcga cagcagcact acggacgcga cgccggtgca gcgggcagcc
    14041 tcggcagcgg cggcaatcgc cttctcgagt gtgcccgagc gctcgtacgc gacctttccg
    14101 gcgagtgtcg cggcgaaggc ctcggaggac tcgccgatca gataggcgcg ggcgacgcgc
    14161 gggaaatagc gggcgagcgg ctctatcccg ccttccttgg gcttgccgcc gacgatccag
    14221 tggatgccgc cctcgaaggc ggcgagcgcc ttctcggtgc tgtcggcgtt cgtcgccttg
    14281 ctgtcgttga tgaagatgac ctcaccgagc cggccgacct cctccatgcg atgcggaagg
    14341 cccgggaagc ttgcgagccc cgcctgcatg cgggagggtt gccacacttt cagggcgacg
    14401 ccgcacgcgt cgagccgcgt ctggagggcg cgcagcgctg tgagggcggc aagcgcgttc
    14461 tgtacgttgt gctgcccgcg caacgagccg atgccggccg tcgaggcgac ctcggcgctg
    14521 gtcgcggcat gcccctcgcg ctcgatcgcg aagagcgtgg tgccgatcgc atagaggcgc
    14581 ggcgtgaggc ccgagccccg gccagcggtg aagccaaaga gacgctccga gggctcgatc
    14641 cgctcggcga tctcgcgcga cacggcatca tcgccgccgc ctccgcgtga tgcaccagcc
    14701 gctccttgat ggccgaatag gccgccatcg tgccgtgccg gtcgaggtga tcgggcgaga
    14761 gattgagcag cacgccgacc gtcgctccac ccgaacccga caggctcggc gcgagatcga
    14821 tctggaacga gctcatctcg atcacgtgga tgcggtcggc acgcggcggc tcgagatcga
    14881 ggatcggcac gccgatgttg ccgcccatct gcacatcgtg gccgagcatg cgcaggaggt
    14941 gcgccgtgag cgcggtcgtc gtcgatttgc cattcgtgcc cgtgaccgcg acgaggggtg
    15001 cgcccggcgc atgtcgcgcc cgctcgcgga gaaagagctc gatatcgccg atgacctcga
    15061 cgccggcgcg gcgcgcgagt gtcacggtgt aatgcggctc aggatgggtc agcggcaccc
    15121 ccggagcgag gacgagcgcg gcaatgcgtg accagtcgac gcccgtgagg tcgacaatgt
    15181 cgaagccagc ttggcgggca ctggcacgac ccgtctcgcc gtcgtcggcg gcccatacgc
    15241 tggccccccc ggccttcagc gcgcgcgccg tcgcaagccc ggagctgccg agcccgaaca
    15301 cggcgacggt tctgcctgcg aacgtggtga ccggaatcat ggctccaatc tacaacatcc
    15361 ttgcgcgact atgcgctgag tctcgttgtc tcaaccgccc gcccctgnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnngtaga gcgagccgct gcgcgtgatc cacaggcggc cggcggcgtc gacggcagca
    15661 ccctgcgcct cgatcgggag cggaatggag ccgagcgcca cccgctcgtc caaggtcgcg
    15721 ggaagacctg cgatgtcgaa gcgccagagg cgggcacccg gctgcttgtc gtagcggccg
    15781 agcaggatcg acccggttcc gccggcagca aacgagcccc tgacgagccc accgagcggg
    15841 atggtgcgca tgacacggcc gatgcctgga gcgctcggga cggcgacctc gatctcgaag
    15901 agcgcacctg tatcggcgac ccagatacgc cccggccctg cgcgcgcgag ccctccggca
    15961 tgaccacagg cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctatatgtac
    16081 cgaggaagat cgcgccctcg gcaaaggtca gaccctgcgg cacatagccc tcgtcgagcc
    16141 cgggcgccca gatgcgcgtc gagatcgccg cggcattcgg cggctcgctg aggacgcgcg
    16201 tcgtgtacgt cggcgcagtg ccgagaggtt cggcgcaagc gtcggtagcg acgacctgca
    16261 gcacaaccgc cagcacgatc aacccgctga gtgccgatcg cctgaggttc atcgaggaga
    16321 ctttcctcgc ctcgcattcc atcgcatgtg attcccccct tggtttcact cacacctttc
    16381 gccgttgcaa taaaccggcg gacaactata gtcgcgccgg aatttgccgc cggcgggcgg
    16441 aggcgcctct gcacggggcg gcgctgccat gtgcagctcc cacccgacct acgagcaccg
    16501 caatcgagag ccgggccttg tgccggcgcg atcccaaagg ccgaaaggac gtctatgttc
    16561 gtctctcccg catttgccca gggcgccggt ggcggcagtg acatcttcgg cctcctgatg
    16621 ccgatgctgc tgattctgcc gatattctac ttcctcatca tccggccgca gcagcagcgc
    16681 gtgaagcaac accgtgaaat ggtcgccaac atccgccgca acgacacggt cgtcctgtcg
    16741 ggcggcattc tcggtaaggt taccaaggtg aaggacagcg acaacgaggt cgaggttgag
    16801 atcgccgaca acacgcgcgt ccgcgtgctc cgtcacacaa tcgccgaggt gcgcgccaag
    16861 ggcgagcccg tcaaggacag cacgactgcc tgagcggccg gccgttccgg tcgtcgccct
    16921 tcccgccacg cctcgcccgt tgaggaccgc ctctcatgct gcacttcgcc ccctggaaga
    16981 tctggtccat catcggcgcc tgcctcgtat cgctgctgct tgcgatcccg aacttcctgt
    17041 cgaaagcgac gctcgaccag ctgcctcctt ggcttccgaa gaagcagatt tcgctcggcc
    17101 tcgacctcag gggcggcgcc cacatcctcc tctcgatgag cacggacgag gtccgcaagg
    17161 cctggctgca gacgatcgcc gaggatgcgc gcaagcgcct agtcacagcg aagaacaagg
    17221 acggccagac gctcggctac gagggccggc ccgccgtggt cggcaagatc gtgcaggtgc
    17281 gtccggcgcg tcccgaggat ggcccgcttg ccatcactga gctgaagaag atgatccagc
    17341 cgctcacctc cggctcgttc atcggggtcg gcggcccgcc cgacctcgag atcaccgccg
    17401 atgcctcggg cctcataacc atttcgccga ccgagcaggc gatccaggac cgcatcacgg
    17461 ccgcgatcag cgccgccatc gagacggtgc gccgccgcgt cgaccccaac ggcacagtgg
    17521 aagccaccat cgtgcagcag ggccgcgacc gcatcctcgt ccaggtgccc ggcatcgagg
    17581 atacgaccaa gctcaaggag ctgatcggcg agaccgcgct gctgacgctc cacctcctgc
    17641 atccgagcat cagcgccgag gaggcgaagg cgcgcggctc gacgccgacg ggctatcgca
    17701 tctatgaatc ggccgacggc ctgcgtggca gttacctcct cgccgagccg gcgatccgcg
    17761 gcgacgacat cgccggggcg cagcccgcga cggactcccg caccggcgag cctgtcgtct
    17821 ccttccaatt gaagaacgcc ggcgcccgcg tcttcggcca gctgacgacc gacaacgtcg
    17881 gcaaaccgtt cgccatcgtg ctcgacggca aggtgctctc ggcaccggtt atccaggaca
    17941 agatcctcgg cggctcggcc cagatctcgg gccgattcag cttcgaggaa gtgacgaagc
    18001 tcgctctcca gctgcgctcc ggcgcgctgc cggcgaagct caccatcatc gaggagcgct
    18061 cggtcggacc gacgctcggc gccgattcga tcgaggccgg atcgcgcgcc ggcatcatcg
    18121 gcggcatcgt caccatgatc atgactatcg ccgcctacgg cacattcggc atcttcgcgg
    18181 tgatcgccct cctagtgaac ggcctgatga tcgtcgccat catgtcgctg atgggctcga
    18241 cgctgacgct ccccggtatc gccggcctcg tgctgacgat cggcatggcc gtcgatgcca
    18301 acgtgctgat ctacgagcgc atccgagagg agttgcgcgc cggccgcacg gcgatcgccg
    18361 cgatcgatgc cggcttctcg cgcgcgatcg tcaccatcgc cgacagccag ctcaccacgc
    18421 tcgcagccgc gatcatcatg ttctggctcg gctcgggccc gatccgcggc ttcgccgtca
    18481 cattgaccat tggcatcttc acctcggtct tcaccgccgt gacggtgacg cgcttgctcg
    18541 tctcctggtg gctcaagaac gcccgcgcca ggcagaagac gatcgaagtc ccgatctgac
    18601 ggcgcggcca tccggccctc gcgccagcac tcaaattcac cgggcatgcc ccgccgcacc
    18661 gacgcacacc aggaagcact catggtcccg gttccgcact tcaagggttt cgacttcttc
    18721 ccccacggca cgcgcatcaa gttcatgcgc tttcgcgaca tctgcttctg gggctcgatc
    18781 gcggcgatgg tcatctcgct cgctctgttc ctcaccaagg ggctcaacta cggcgtcgac
    18841 ttcaagggcg gctcgatggt cgaggtgcag gcgaagacag ggaaggccga cgtcgcggca
    18901 ctccgcacca agctctccgg catcggcctc ggagacgtgc agatccagcc cttcggcaag
    18961 gccggcgacg tactgatccg catcgagcag cagcccggcg gcgagaaggc gcagcaggat
    19021 gccgtcgcaa aggtgatggg gctcatcaag gacgagtaca accagcgcca ggtgcaggtc
    19081 gtcggnnnnn nnnnnccgcc gtatcgagcg agcttcgcac caccggcatc atcgccgtga
    19141 tcgcctcgat cctcgcgatc atcatgtacg tctggttccg cttcgagtgg cagttcgcgc
    19201 tcggcggcgt gctcgccctc tcgcacgacg tgctgctgac cgttgggcta ttctcgctgt
    19261 tccagctcga gttcgatctc tcgatcgtgg ccgctctcct caccatcctc ggctactcga
    19321 tcaacgatac ggtcgtcgtc tcggaccgca tccgggagaa ccttcgcaag tacaagcgca
    19381 tggcgctgac tgagctcctc gatctctcga tcaacgagac gctctcgcgc acggtgctca
    19441 ccggcatgac gacgctcgca gtgctcgtct gtctctggat ctggggcggc ccggtgctgc
    19501 gcaacttcac attcgccatg ctgttcggtg tgctggtcgg cacctactct tcaattttca
    19561 tcgcggcgcc tctcctcgac tacctcggcg tccgccgcga gaccgtggtc ggcaccgaga
    19621 aggcaaagcg cgcttgatcg cgggcatttg gccatgaaca gcgacggcca agcggcagca
    19681 gcaggctccg gacgctatcc ggggcaggcg ccgatcgatg cctacggcaa tggcggcttc
    19741 cgcttcgccg gcatgagcca ccgcggcgcg atcctctgcc tgccgagcgc catcctcgtc
    19801 tggcggccga gcgcagtcga cgacattggc gaacccgatc tcgccgcagt gcttgccgag
    19861 ccgcgcgccg tgaagcacct cctgatcggg acgggccttc gctttgccct gccgccgcga
    19921 gcgatcctcc gccgcctcgc cgatgccggc atcggggccg agcccatgga cacgggcgcc
    19981 gcctgccgca cctacaacat tctgctctcc gagcggcgcc cggtcgcggc agcgcttctc
    20041 cccgtcgcct gaacatgcat ccgtctcgcc gccgctgaag gagcctgcat atgacccgca
    20101 atctccgccc cgagaccatc gccgcccagg cctgcggcat cgtcgacgag gcgaccggcg
    20161 ccattgtgct gccgatcggc gtcgcgacga ccttcatacg cgaccccgac aaccagtacc
    20221 gcaagggcta ctcctacggc cgcgccgaca acacgaccgt ccggctcgcc gaggatgtgc
    20281 tgacgaggct cgagggcggc gcgctctcag tcgtgctcgg ctcgggcatg tcggccgcga
    20341 tcgcctcctt cctcgccctc gatcgcccgg cccacctcat cgcgccgaag atcatgtact
    20401 ggggattgcg cgcctggctc ctcgacgacg cgcctggcta cggcatcacg acgaccttcg
    20461 tcgacacggc cgacctcgat gccgtgcgcg ccgccatacg gcctggccag acccgcatga
    20521 tctgggccga gacaccgtcg aaccccatgt ggggcatcgc cgacatcgcc gccctcgccg
    20581 agatcgcgca caaggccggc gccatcctcg gcgtcgattc gaccgcggcg acgccggtgc
    20641 tctcgaaccc gatcgcgctc ggcgccgacg tcgtcatgca ctccgccacc aagtacctga
    20701 acggccattc cgatgtgatc gccggcgcgc tcgtcctcgc ccgatccggt ccctacgcgg
    20761 atcgcgttct ccgcgtgcgc ggaaaactcg gcctgatcct cgggcctttc gaggcgtcgc
    20821 tgctcgtccg cggcatgcgg acgttacacg tgcgggtccg tcaccagacg gcgagcgcca
    20881 tgaagatcgc cgagcatttc gcggccgaca ggcgcgtcgg cgcggtgctc tatccgggcc
    20941 ttcccggcca tcccggccac gccgtcgccg cgcgccagat gagcggcggg ttcggcggca
    21001 tgatctcgat ccggatcgcg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgcgcctccg tcgaaggtcc
    21121 gggcacgccc tgccccgctg atctccttcg cctctcgacc ggcctcgaag acaccggcga
    21181 cctcatcgcc gatctctcgc aggcgctcga gtgaggagtg ctcgcggtca tctatggggc
    21241 tcgtattcga ttggcgggtg ggtatgagcc gcatcggaca tcaagctgca cttcccactc
    21301 tccccgtaag gatggggaga ggggatttgc gcgacacgtc aattgtcgga aagtggcccc
    21361 tatccgacac cctgtgtcct cggcaaattg gctgcaccgg ctaggcaaca tcccgccttc
    21421 gggcctatca atgggccagg atcccactcc atcccgagca cgcaccatga caacctcgtc
    21481 ccgccgcccc gccgattgcc gtgatctcgc cgacgtgcgc gtcgagatcg accgctgcga
    21541 caaggccctc gtcgacctca tcggcgagcg cttcggctac gtcgagcgcg cctggcagat
    21601 caagctcggc ttgaagcagg aggcgaacgt cccctggcgc aaccagcagg tgatcgaccg
    21661 ggtgcgcgcc cgcgccacgg agcggggggt gccccccgac ctctgcgagg cgctctggcg
    21721 gcagatgatc ggctggttca tccagtacga ggaagagaag ctgcgtcacg agatcgaggg
    21781 ctcgggcaaa ggcaaggacg cgacgggctg aggctgtggc gatcacgcgg cgcgccgcga
    21841 tctccggcgg gatagggctc gccttcgcag gcgctggtcc cgcagtagcg cagcctcgtg
    21901 cgcgaaaacc gcgcctgccg cccggccgca atcccggcgg cgtgcccgtc gccgtgatcg
    21961 acggcggcat cgactacacg cgcgccgaca tcgccgggcg ccttgcccgc gacggcgagg
    22021 gcgatctcgt cggctgggac ctcgacgaca acgacaaccg ccccttcgag cgcgcctggc
    22081 cgggcggtgg cgagccacaa ggcacgctac ttgcgacgat catgcttgcc gaagccggcg
    22141 cttccaccgt cgtgccggtc cgccgccgcg accgcgacgg cctctcgatt gcgcgcgccc
    22201 tgtcgttcgt ctcgcagacg cccgcccgct tcgccgtcct ccccttcgct agcgacgatc
    22261 ctgacttctg ggcgaagttc cgcgaggccg cgacgcacct ttcgggcgtc atggtgatcg
    22321 tgccggcggc gatgcagggc ggcgcgtccc gagatctcgc ggcgacgccg agctacccgg
    22381 ccgccttcgg tctcccgaac gtgctcagcg taaccgccgt cgatacagcg ggtatgctgc
    22441 tccacggggc tccacgcggt gccgcatcga tcgacctcgc agtgcctgct cacgatacgg
    22501 tcggctatgg ccgcgacggc gagaaggttc ccattcccgg cacgctcggc gcggtgatgc
    22561 gcgcagccgg tatggcagcg cggcttcagg agcgcgagcc gcacttgcgg ggagccgccc
    22621 tcaagtcgcg cttgctcgcc gatgcgcgcc cgttgcccga tccgaccatt gccagagccg
    22681 gctggatcgc tgatgcccgc cgccgctact ggctcgagta gccttccggc cgctactgcg
    22741 gcgcgaacgg gttcttccaa ccacccggtg cctttcgctc cggaaaggcg acgccgagat
    22801 agtccaagat cttttcccgc tccgcccctt cgagcttcgg catgccatgc cggtcgacca
    22861 tccaggtgag gctctcgtcc catcgctcgc gggacatgcc ctgcgaggcg acgatcgcga
    22921 agccgtggca ggcggtgcag aaatagaacg cgtcgtcgcg atgcggtccg gccgggaact
    22981 gctccggccg ctcgtcgttg cctttcttgc gggcctgagc gaccgcgtcc ttcgagagcg
    23041 gcagcatcgc gccgccgctc ccgatcaggg cgagcgccgc cgccatggcg gctgcccgca
    23101 tcatacgacg tgccgacggc atgaggctca tccaaccagc accgcgatac ggtgcatcgg
    23161 attgccgccg tagccctgcg gattccagtt gccggcgatg tggggctgca tgacgccgcg
    23221 cgtgtcggtc gcgcgcaccc agacctcgta gtagccgtcg ctcggcaggg cgaccttggc
    23281 cgtccagcgc cgccaatcga agcggttgcg ggaattggcg aggctcgcgc ggctccaact
    23341 cgcgccgaag tcggtcgaga cgtcgacgcg ggcgacatcg cggtcacccg cccaggccgc
    23401 gccgcgaagc gcgagcgagc gcgtgccggc cgcgagcttc gtgccgttca tcggattggt
    23461 gatgatgccg cgcacgggca tcgactcgag gatnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23521 nnnnnnnnnn ncgcgacgcg ataggaggtg cccgtcatgc cggcgccgtc gtgcaccttg
    23581 tcgcgcaagg tgatgcgggt cagccatttg tgcgatactg agcccggcca gcccgggatg
    23641 acgagccgca gcggcgcgcc gtgaatggcc tcgaggggct tcccgttcat cgcccacacg
    23701 agcagcgatt gcggctgcat cgccttggcg acaggcacgc cgcgcgagag gacgtcgcgc
    23761 ttggcatccc ccgagagatg cacgtccgcg ccgtagtgcc ccgtgtagac cgcagagggc
    23821 ttgaggccgg ccgccttcag cacgtcggag agcaggacgc ccgtccattc cgcgcagccc
    23881 gcgccaccat tcgtccactg gttgccgcgc gcctgcggcg tgaagaacga gcgaccgttg
    23941 ccgccgcatt cgaggacgct gcgcagtgtg acggccttga accgcttctt gatgtcgccg
    24001 agcgcgagtt cgagcggtgt tgcgacctcg ccatcgatct tgagtttcca ggcgtcgacg
    24061 tcgcccgcga ccttcggggg tgttccgttg ttacgaatga agaatttcga gacaggcgtc
    24121 gtatcgtcgt cgaggagatg ctcgggcgtc tccgcgacga gcggcttgtc gccgagaagt
    24181 gtgaggcccg ggtccttgcc tggaaaatcg aagcgcttcg gcgcggccgg tgcggtcggc
    24241 gctgctgctg ccggagctgg cgccgcgggc ttcggagcag gcttcgggtc ctgcgcgagc
    24301 gccgccggaa cgaggcccgc cggcatcgtc gcctgataga cgatcgtgcc tccgaccgcc
    24361 gccgtcatcg cgccaagccc tgcgccgccg agaaatccgc ggcgcgacac atccgtcttg
    24421 cggccgaaga tcacggcatc cgcgcgatcg gcatcgtcgg tgtaaagttc atcgacgctc
    24481 cgttcgcgct tgccgagctt cggagatggt ttcccgtcct tcatggcgct tctccctgct
    24541 ttgatatcaa ctcttttgaa tatgagccta gcgacgcact ccgggtttgc caagccgctc
    24601 gcctcgccaa tccgcccgat cggcatgccg gtgagggccg gcacgccgag cacctgggag
    24661 tgactgctcc cgttcccttg cccagtgcca tgccccctcg cagtcgcaag gatgttgagg
    24721 gtagcgcgac caagtcgcaa ccggccacgc gggtccccgg gattcggcag tggtggttcg
    24781 cactcatgcc ggcgcgcgcc cgatcggtgc tcctgtagca tatgccgatc gtgctcggtt
    24841 ggacacgctc ttgccttacc cgtgcgcgat tgttgcgtgg ttgcaccggc actgtggtgc
    24901 cgacacttgc caagccatcg ccgtcgctgg cacataggtt cggacagcag tcacccgggc
    24961 cgaggcgccg tcgattgtgg caacgggcgc gacggcaggc agatgggatg gggagcatcg
    25021 aaggcatggg cggggtgggc ctctgacatg accgtcgccg atcaacagac cgagtggtat
    25081 ctcgcgcgcg acggcaagcc ctatggcccg atgagcgcct cggagatggc gaaggtcgtg
    25141 gacctcggct atctgcgcga gagcgatctc gtgtggcggc ccgggttcgc cgagtggttg
    25201 cctgctttcc aggttttccc gccgcagccc gacccggtcc cggagccggc gtctgccgct
    25261 cccgctccgg ccccgccgcc ggccggctcc gcattcaggc cggcggatca gccagtc
//
LOCUS       DAORNH010000025        25130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1524_length_25130_cov_2.671545, whole genome shotgun sequence.
ACCESSION   DAORNH010000025 DAORNH010000000
VERSION     DAORNH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 25130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25130
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1524_length_25130_cov_2.671545"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PK264_04460"
     CDS             complement(<1..467)
                     /locus_tag="PK264_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD75179.1"
                     /translation="MAEHANGRLPILATEALSKSFGGLRAVRDVTLALHVKELHAVIG
                     PNGAGKSTFVNLLTGQLAPSSGRIRLEGRDITGTPAWRMPHLGIARSFQRTNILKSMS
                     ALENVRLAAQAVRLPASRMLRAPGREASLIDAARAALERAGLAGAEAAIAGTL"
     gene            complement(460..1056)
                     /locus_tag="PK264_04465"
     CDS             complement(460..1056)
                     /locus_tag="PK264_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD75180.1"
                     /translation="MPAFGLSIDPQSLPRSKRSWPAYYVALTQLVLTYLILAFLLRSL
                     FGRVLEGIRVNEHRMEAMGFNTTRYKLAAFVVAGTLAGAAGHMWSLHNGSVNPELVSW
                     HKSAEALLMILLGGIGSLAGAIVGAFAYMGLNELGQVVQLMTEQKPIFGVIVVRKLLF
                     EGIVILAAVILLRNGIMGIRLWRSKGSASSGRAGGAHG"
     assembly_gap    1655..1688
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1689..2006)
                     /locus_tag="PK264_04470"
     CDS             complement(<1689..2006)
                     /locus_tag="PK264_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD75181.1"
                     /translation="MPITAGFSYAAYRFMVIGLCLALAGGLFFWIERTRMGAIIRAAA
                     EKPEMVDVLGIDARRVHLTVVAVGTALAMTAGALAAPLTSVYPNMGDGLLIISFVVVV
                     IGGL"
     gene            complement(1958..2386)
                     /locus_tag="PK264_04475"
     CDS             complement(1958..2386)
                     /locus_tag="PK264_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75182.1"
                     /translation="MIATLMSQLLNGVQYGLLLFLIASGLTLIFGVLGIINLAHGSLF
                     MIGAYTAFLVTKYSGSLWFALPVAIVAGLVLGSLLERFLFRFFYHREHLDQVLVTFAL
                     ILIFAPSPSVVTSTPCQCPRSSISRCRSRPASAMPRTASW"
     gene            complement(2456..3658)
                     /gene="boxA"
                     /locus_tag="PK264_04480"
     CDS             complement(2456..3658)
                     /gene="boxA"
                     /locus_tag="PK264_04480"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HRD75183.1"
                     /translation="MTATATLKKQHLIDPEVCIRCNTCEATCPVGAVTHDDNNYVVDA
                     EKCNYCMDCIAPCPTGSIDNWRVVRPEKAYSLEEQLGWRELPKQEDLGEGSADAQQAV
                     EDEVEKLLASAHAGTGGKAVAPASASKPTVNLYRRDKPAVATVQGNFRITGAETESDV
                     RHIVLSFGSTVVPVLEGQSIGIVPPGKRTDGKPHDIRLYSIASARDGEKRNANNVGLT
                     IKRVTGGVGSNYMCDLKVGDKVEVTGPFGATFLMPNDANANIIMICTGTGSAPFRAFT
                     ERRRRAMANAPGKLVMFFGARTPAELPYFGPLQKVPASLLEKHLVFSRIPGADKEYVQ
                     DRMRAEAVSIAKLIASEATHIFICGLKDMETGVEEALADIARKHGLEWSELKPKMRQS
                     GRYHVETY"
     assembly_gap    3810..3819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3820..>4689)
                     /locus_tag="PK264_04485"
     CDS             complement(<3820..>4689)
                     /locus_tag="PK264_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD75184.1"
                     /translation="LSDHPVDIDVTEATYEAISETLFGGMAMHESLKIRSAVATYLNA
                     APWEIVSALMRRPADAWHPGRLQLNAATVDMRNAILALYTRWQAEGGEGRHLLSHLVT
                     ATDKGEPMSETQILNNLITFLNAGHETTAKAITWTLYVLARHPVWQEAARREIAEVVG
                     DAPIAAEHIEKLSLTRMILEEAMRLYAPAPVLTRIATRSITLGAHTIDADTLVFIPIW
                     AIHRHRRLWDEPERFDPMRFLPEKRASYRRTQYMPFGFGPRICIGATFAMVEGVVMLA
                     AMLRGARFAWNGRE"
     assembly_gap    4690..4736
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4737..>5029)
                     /locus_tag="PK264_04490"
     CDS             complement(<4737..>5029)
                     /locus_tag="PK264_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD75185.1"
                     /translation="PPALFLYHFLRNPLRCLPETVYEEPIVFSADRRTAWATDPDLIE
                     AIFLDSHDVFPKPPLEARVFQDPLGNSILTAQGEAWRWQRKAVAPLFRPKDILA"
     assembly_gap    5030..5117
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5395..5404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5405..6776)
                     /gene="boxB"
                     /locus_tag="PK264_04495"
                     /pseudo
     CDS             complement(5405..6776)
                     /gene="boxB"
                     /locus_tag="PK264_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491285.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
     gene            complement(6833..7198)
                     /locus_tag="PK264_04500"
     CDS             complement(6833..7198)
                     /locus_tag="PK264_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRD75186.1"
                     /translation="MSGTIKSHRDLLVWQKSMDLAVGAYRLTAAFPVDERDGLTSPIC
                     RSCASIPANVAEGYRRESPGAYAQQLRIAHESLKAFETHIELAHRVGLLDQDVLSSQL
                     SASEEIGKMHRSLMRAVDR"
     gene            complement(7235..8988)
                     /gene="boxC"
                     /locus_tag="PK264_04505"
                     /pseudo
     CDS             complement(7235..8988)
                     /gene="boxC"
                     /locus_tag="PK264_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734960.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
     gene            9172..10050
                     /locus_tag="PK264_04510"
     CDS             9172..10050
                     /locus_tag="PK264_04510"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRD75187.1"
                     /translation="MDTAPITRRIGTRVRAGRARIGMTRKQLAAAAEVSERYLNELEN
                     GDANASVAILAKVADALGLDLVSLLSADDASPGVAAQPAFEPLASLMAGMSLAEQQAA
                     LPVLDTFITDRRRRLRGIALLGLRGAGKSTIGRLFAARHGLGFISVTREIEARAGMSL
                     AELFNLGGPDAYRSLENEVIAELTRRPDRLVLETAGGIVGNNQALDMILGAFKTVWLK
                     ATPEEHLARVSGQGDTRPMRGHPKALEHLRALLAAREPDYARAESVIDTSGRTVEDCV
                     ADLERIAGGVVTQDAA"
     gene            complement(10169..>10847)
                     /gene="ilvC"
                     /locus_tag="PK264_04515"
     CDS             complement(10169..>10847)
                     /gene="ilvC"
                     /locus_tag="PK264_04515"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRD75188.1"
                     /translation="NLIEPRADLDVGMVAPKGPGHTVRSEYQRGGGVPCLLAIHQDKS
                     GNAHDLFLSYAAAIGGGRAGVIETSFKEECETDLFGEQVVLCGGLVELIRAGYETLVE
                     AGYAPEMAYFECLHEVKLIVDLIYEGGIATMNYSISNTAEFGEYVTGPRIITDETRAE
                     MRRVLADIQQGKFTSEWIRECKAGQPKFKATRRLNDAHPIEEIGTKLRAMMPWIKEKA
                     LVDKSKN"
     assembly_gap    10848..10876
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10877..11207)
                     /locus_tag="PK264_04520"
     CDS             complement(<10877..11207)
                     /locus_tag="PK264_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75189.1"
                     /translation="MRVYYDRDADINLIKSKKVAVIGYGSQGHAHAQNLRDSGVKDVV
                     IALRKGSQGVKKAEGAGFKVMEVAAAAKWADVMMMCTPDELQADIYKDHLHENMKKGA
                     AIMFAHGL"
     assembly_gap    11210..11322
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11366..>12161)
                     /locus_tag="PK264_04525"
     CDS             complement(11366..>12161)
                     /locus_tag="PK264_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75190.1"
                     /translation="SIADAMKREAGVTTHHLNEKGLDGLAWLGTGNIYSPEGRNIRHL
                     ITVAHECGHIFLQSSGPGLLLPSHVQEMEAESYAHQALVEYGMIVPKQRTEWGRAYVG
                     TWVEKDRARGIPIDPRVIEYVAGFRSPYEPLRMVPETWRQFRALPPPASPPVYVPPIK
                     PEVGTTTLPTWQEWLKATTPQADEPTKPTAGERVWDVLAGVCWWGVWGLLLSRAAFAT
                     LDQVFPIEDYRKYDISTLEVGVCLTIGLATGSLGALWRSVRAMRGT"
     assembly_gap    12162..12171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12368..12691)
                     /locus_tag="PK264_04530"
     CDS             complement(12368..12691)
                     /locus_tag="PK264_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HRD75191.1"
                     /translation="MVSATAGKEAVCAFDRAELTQILDLYGRQVAAGEWRDYAMDFDR
                     EKAVFSVFRRSSEMPLYRIEKCPKLARKQGAYSVVAAGGQILKRGHELARVISVLEPK
                     IRVVI"
     gene            complement(12845..14020)
                     /locus_tag="PK264_04535"
     CDS             complement(12845..14020)
                     /locus_tag="PK264_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRD75192.1"
                     /translation="MTTPRLANMAPPETYRLRIPGPTAVPERVRLAMARPVQAHRGAE
                     FKAILAAAVERLAPVFGTRNRILLHSSSGTGMMEAALVNVLAAGERILVIENGQFGER
                     FSQIAKAYGVVVDPLVVPWGEEADPADVAKRLASADYRAVVMVHNESSTGVAADLAAV
                     GRVVAKTPALLVVDSVSGLGGMPIGQDAAGVDVLVTGSQKALMSPPGIGLASVSEKAW
                     TVVGRDDRLPRFYFDFRKARDSAEKGDTTFTGPVSLVYALNEALAMMHEEGLERVFAR
                     HARLSRALKAGAAAIGLENFTRSRHQSTTVAVFHAPKGYDGGAIVRGMYERHRSVIAG
                     ARNRLSGKVIRIGTMGAVTDSDVLTDLAHLEDVLASLGLALEAGAGVAAAARALAAH"
     gene            14400..14702
                     /locus_tag="PK264_04540"
     CDS             14400..14702
                     /locus_tag="PK264_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75193.1"
                     /translation="MHAQDVTLALFTAVNLIRVFSYLPQIRCIARDTTGATCISYTTW
                     AMWVLGHATTLLYAIVNLGDPVLATVSTINMTCCLTVIALTAWKRRVVAMAGSIAA"
     gene            14916..16745
                     /locus_tag="PK264_04545"
     CDS             14916..16745
                     /locus_tag="PK264_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD75194.1"
                     /translation="MIETPERALASVGLFKGLSREALATLTRRAVMREVPRGHVVVRQ
                     GEAADALHIVVSGRFDVTLEGRPRPIAEITAGSPIGEIAFLAGGTRTATVTAVRDGIV
                     MTLGRADFDELLKTNPDAARIMMATVAGRLAAATATMAPRAAARPKTIAIITAGGRPV
                     PMAFLQKLREVVDRRSRSLIVGSETVGRVLDGIRGDAEMTTALNAIEADYDFVVYLAD
                     PALTPWSEKAIRQADLVLRIADVRTSHGALECGENDLERFATRLHQSAGQRLVLVHET
                     QSAVSGTRAWLQDRSVAQHHHVALDDAASFERLFRFIAGTARGLVACGGAAFCAAHIG
                     IYQALGEAGVEIDMLGGTSGGAAMTAGFAAGFTPAAMSEAVRDIFVTNRAMRRYTWPR
                     YSLLDHTHFDRMLERYYGRIDIEDLWLPYFAVSTNLSRYALHCHASGDLFHAVRASAS
                     IPALLPPLYTEDGQMLVDGCLLDNVPIRMMRELKSGPNIVVAFEVPELQRFKVDYARL
                     PSRAELLKRAAMPWRREALPDAPGVASVLMRSLMANRQDFERHLKPDDLLIVPPLPDD
                     MSILDWHRHGELVQSAYGWMRDEILRQRARGATALGGGASRLP"
     gene            16785..17945
                     /locus_tag="PK264_04550"
     CDS             16785..17945
                     /locus_tag="PK264_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD75195.1"
                     /translation="MDFDLSEEQQLLKDSIDRLLASRYPDVKTRAGHMAAPKGYSAAI
                     WQEIADLGLLGIPFAEAHGGLGQGYVEIMIVMEAIGRNLALEPYLPTVVLGGGCLRHA
                     GSDAQIGALAPDIIAGKTTLALAHLEKQARYDLADTAMSAKADGKGGYVLEGEKCVVL
                     GGDSADKLVVTARTSGDRRSSSGIGLFMVDATAGGISRRGYQTQDGMRAADIAFSGVR
                     VGKDGVLGDPAGGYSVLARVVDEAIAALAAEAVGAMEGAHLLTVEYLKTRKQFGVPIG
                     SFQVLQHRSVDMFTNLEQSRSMALLASMMAAEPDAAERARAMSAVKVQIGRSGRFIGQ
                     EATQLHGGIGMTMEYKSGHYFKRLAMIDLAFGPADHHLKKLAEAGGVLSEAA"
     gene            17942..18163
                     /locus_tag="PK264_04555"
     CDS             17942..18163
                     /locus_tag="PK264_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HRD75196.1"
                     /translation="MNEAPVRPPIRRLVGTVALLVFLVVYALAVMMVAITVLPQLGKW
                     AEPVFYLVGGLLWIIPAGFLIRWMYRPGT"
     gene            18281..19285
                     /locus_tag="PK264_04560"
     CDS             18281..19285
                     /locus_tag="PK264_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75197.1"
                     /translation="MKLSIAARALSLALATGLGLTLLSPPDVRAEANTVRAAKQWGLG
                     YLQYMLMEDMKLVEKHAKAAGLGDVKVEWNTFRSSDVMNDALISGNVDFVSLGVPGLM
                     TIWDRTKGKLDVKGVTALNSLPLVLNVRAPEVKTLADFTEKHRIAVPAVKVSVQAILL
                     QMAAEKLHGAGKHTALDAITITMAHPDATVAMLSAQTEVSANFSSPPFYFRQLKQPGI
                     RRLTSSTEINGGPLPFNVVAATSKFRSENPKLYKAFLAAMAEATELINKDKRWAGEAY
                     LRIAKDKTPIDEFMTIMNDPEINFTLAFASFDNMINFMTRTGNFKNKPASSKELMFQD
                     "
     gene            complement(19673..19945)
                     /locus_tag="PK264_04565"
     CDS             complement(19673..19945)
                     /locus_tag="PK264_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD75198.1"
                     /translation="MDILELHRSRYSDFGVARLLVFGSRARGTPGPASDIDILVEFTE
                     PTTFDRFIDLKDFLEGLLGLRVDLVTTKALRPELKDAIEREAIRVA"
     gene            complement(20481..>21142)
                     /locus_tag="PK264_04570"
     CDS             complement(20481..>21142)
                     /locus_tag="PK264_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD75199.1"
                     /translation="RCMAVSVAVPVGKATADKLVSKLIPRVEGLKIGPSTSADADFGP
                     VVTKAHLEKVKQYVEIGVKEGAKLKVDGRGFRMQGYENGFYMGGCLFDEVTPKMRIYK
                     EEIFGPVLSVVRAKDYDEALRLPMEHEYGNGVAIFTRDGDAARDFAARINIGMVGINV
                     PIPVPLAYYTFGGWKKSAFGDLNQHGPDSVRFYTRTKTVTSRWPSGVKEGAEFTIPVM
                     K"
     assembly_gap    21143..21152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21153..21979)
                     /locus_tag="PK264_04575"
     CDS             complement(<21153..21979)
                     /locus_tag="PK264_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD75200.1"
                     /translation="MATELYHFIGGKRVKGTSGKFGDVFWPMTGEVAAKVPLASKAEV
                     RAAVENAKAAQPAWAATNPQRRARVLMKFVDLVARDNEKLALALAREHGKTVADAKGD
                     IQRGVEVAEFACGIPQLMKGEYTEGAGPGIDIYSMRQPLGVVAGITPFNFPAMIPMWK
                     FAPAIACGNAFILKPSERDPSVPLMLAELMMEAGLPAGILNVVNGDKEAVDAILDDRD
                     IEAVGFVGSTPIAEYVYSRGCAAGKRVQCFGGAKNHMIIMPDADMDQAVDALIGAGYG
                     "
     gene            22196..22669
                     /locus_tag="PK264_04580"
     CDS             22196..22669
                     /locus_tag="PK264_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HRD75201.1"
                     /translation="MWSKFAKLAAVAVVAAVVTAAPVSAADLIVKYDQSQIIRLDRPV
                     AEIIIGNSTIADVSVQAPQMLVVTGKTFGITNVIILDASRQTIAEHRIIVQRDEVNIV
                     TLHAAAKRQSYNCTPRCNPSLVVGDDLNYFETVAKVSERKIKFSEGANDASGGGQ"
     gene            22885..23499
                     /locus_tag="PK264_04585"
     CDS             22885..23499
                     /locus_tag="PK264_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75202.1"
                     /translation="MTDIVIWMLAIAVSVAALVVAAAAKLHYIHMAVAAVMAIFIALS
                     AVRELRVTTASGAGDAAVASVAARYMGLVWAWGAIVLMITYMFILQWKEWWQFFIAFI
                     VAAGICLFFSATLRSDSAKGADDPGMLSLSRYLALAQAIGTVVTIVGLGVFDGKGFRL
                     GGKMLRFLDPRHTDWAANNVFFFGAIALAAISFTALSALGRSRR"
     gene            23510..24124
                     /locus_tag="PK264_04590"
     CDS             23510..24124
                     /locus_tag="PK264_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75203.1"
                     /translation="MATATDAPSLSRLMPRLAAVHLASVGGIIVGAGLGRPLLVQAFA
                     ALFATVIILVAIAINRPLWGAETAAVAPAEAARRNIRLVALAYAWGALAMLAAYALSG
                     VRWQHGWQYGAGMAVISAITMLVAHRQGDPRSVLARAPALRLLSLVNVLHGAAAIAAL
                     AWLIGSGKLMSPRGDWVANQIFLFGGLVIVALSVFAWLTHQRWR"
     gene            complement(24128..>25130)
                     /locus_tag="PK264_04595"
     CDS             complement(24128..>25130)
                     /locus_tag="PK264_04595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD75204.1"
                     /translation="FFSPSSVAHGGPSLMGEPNASRPHPTSAALMVCTYRRPQAFAKL
                     VATIATLDIPKGLAFSIVVADNNRESAWGSYIQAPLASLPWPVHYGHEPEPGYSNARN
                     AAIKVALERTDAEILLFVDDDMLLSQGWLAGHLASHVEFGCDVVNGRISGVRERFPHG
                     AKLQKCGAGNVSFNRRLVAANGLGLRFDPAFNATGMEDQAFFGRAVALGVDIRQSDHP
                     LIYNYYGDGPPPEEEIVNKMLVTGGMQQNLVARARQEKGVVPAAMLASKGLLFGGKAL
                     GLAGLSRLQRLLGRPDEARRSELSAQKERLKMSGRFRGFSGRMVSRQEVRRGDTDGA"
BASE COUNT         4244 a         8266 c         7952 g         4317 t
ORIGIN      
        1 ctgagcgtgc cggcaatggc cgcttcggcg cctgcgaggc ccgcccgctc gagtgctgcc
       61 cgcgccgcgt cgatgaggct tgcctcgcgg ccgggtgccc gcagcatgcg cgatgccggg
      121 aggcggaccg cctgggcagc gaggcggacg ttctcaaggg cggacatgga cttcaggatg
      181 ttcgtgcgct ggaacgatcg ggcgatgccg agatgcggca tgcgccacgc gggcgtcccc
      241 gtgatgtcgc gtccttcgag gcggatgcgc ccgctgctcg gcgcgagctg gcccgtcagc
      301 aggttgacga aagtcgactt gccggcgccg ttcgggccga tcactgcgtg cagctccttg
      361 acgtggaggg cgagtgtgac gtcgcgcacg gcgcggagcc cgccgaaaga cttcgatagc
      421 gcctcggtcg caaggatcgg cagccggccg ttggcgtgct cagccatggg cgcctcctgc
      481 gcggccgctg ctcgctgagc ccttcgagcg ccagagccgt atccccatga tgccgttccg
      541 gagcaggatg accgcggcga ggatgacgat cccctcgaac aggagcttcc tcacgacgat
      601 cacgccgaag atcggcttct gctcggtcat cagttgtacc acctggccga gctcgttgag
      661 gcccatgtag gcgaaggccc cgacgatcgc gccggcgagc gagccgatgc cgccgaggag
      721 gatcatgagc agcgcctcgg ccgacttgtg ccagctcacg agctcgggat tgaccgagcc
      781 gttgtgcagg ctccacatgt ggcccgcggc ccccgcgagc gttcccgcga cgacgaaggc
      841 ggcgagcttg tagcgcgtcg tattgaatcc catcgcctcc atgcggtgct cgttgacgcg
      901 gatgccctcg agcacgcgtc cgaacagcga gcgcagcagg aaggcaagga tgaggtaggt
      961 cagcacaagc tgggtgagcg ccacgtagta ggcaggccac gagcgcttcg agcggggcag
     1021 gctctgggga tcgatcgaga ggccgaacgc gggcaagata ggcgccgtcc gtgccaccgc
     1081 cgagcttggt gtcgtggaat acgaagtaaa tcatctgccc aaaggcgagc gtcaccatca
     1141 ggaagaagaa gcccttggtg cgcagcgaca gcgacccgac gacaagtgag acgaaccccg
     1201 ccatcgccat cgcggccggc agcgtttgcc agattggccg cggcgctccc tcgggcgaga
     1261 ggagataaac gacataggcg gcaagcccga agaaggcgcc gtggccgagg ctcacgagcc
     1321 ccgtcgcgcc gacaaggagg tgcaggctca tggccagcat cgcggcgatc atggtcgtga
     1381 cggcgaggcc gacataatag ggaggagcgt agaagggcag caccgcgagc gcagccgcgc
     1441 cgattgcgag cccgatccag cccagccagg actgtgggag catgcgtgct ggcgaaatca
     1501 tgctcggcct ccgaagagcc cggtcggacg gaagagaagc accagcacca tcaggatgta
     1561 gaccgacatc gacgagaccg ccgcgatgac gccgccaacg gccgtgccga cctgagcggc
     1621 accgtaggcc ttgccgagcg tatcgatgag cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnga ggccgccgat gacgacgacg acgaacgaga tgatgaggag cccatcgccc
     1741 atgttcgggt agacggacgt gagcggagcc gcgagagcac ctgcggtcat ggcgagggca
     1801 gtcccgaccg cgacgacagt cagatgcacg cgccgcgcat cgatgccgag cacgtcgacc
     1861 atctcgggtt tctcggccgc ggctcggatg atcgcaccca ttcgcgtccg ctcgatccag
     1921 aagaacagac cgccggcgag cgcgaggcag agcccgatca ccatgaagcg gtacgcggca
     1981 tagctgaagc cggccgtgat cggcaccgag aaatcgacga tcgagggcac tggcacggag
     2041 tggaagtcac taccgacggc gatggcgcga agatcaggat cagcgcgaag gtgacgagca
     2101 cctggtcaag gtgctcgcga tgatagaaaa agcggaacag gaagcgctca agcagtgatc
     2161 caagaaccag ccctgcaact atcgctactg gaagagcgaa ccagagcgag ccggaatact
     2221 tcgtcacgag aaacgccgtg taggcgccga tcatgaacag gctgccgtgg gctagattga
     2281 tgatgccaag cacgccgaag atgagcgtga gcccgctcgc aatgaggaac aacagcagtc
     2341 cgtactgcac gccgttcagc agctgcgaca tgagtgtcgc gatcatgcgt cagtccctcc
     2401 cctcacccca aaggacacgc gggctgctct gccggctgcc tcacatgccc cgaactcagt
     2461 atgtctcgac gtgatagcgc ccgctctggc gcatcttcgg cttgagttcg gaccattcca
     2521 gtccgtgctt tcgagcaatg tcggcgagcg cctcctcgac gcccgtctcc atgtctttca
     2581 gcccgcagat gaaaatgtgt gtcgcctcgc tcgcgatcag cttggcgatg ctcacagcct
     2641 cggcgcgcat gcggtcctgg acgtactcct tgtcggcgcc cggaatgcgc gagaagacga
     2701 ggtgcttctc gagcaggctc gccggcacct tttgcagcgg gccgaagtac gggagctctg
     2761 ccggcgttcg cgcgccaaag aacatgacga gcttgcccgg cgcgttggcc atggcgcggc
     2821 ggcggcgctc ggtgaaggca cggaacggcg ccgagcccgt ccccgtgcaa atcatgatga
     2881 tgttggcgtt ggcgtcattc ggcatcagga acgttgcgcc gaacgggccg gtcacctcga
     2941 ccttgtcgcc gaccttcagg tcgcacatgt agttggatcc gacgccgccc gtgacgcgct
     3001 tgatcgtgag gcccacgttg ttggcgttgc gcttctcgcc gtcgcgcgcc gaggcgatcg
     3061 agtagaggcg gatgtcatgg ggcttcccat cggtgcgctt ccccggcggc acgatgccga
     3121 tcgactggcc ctcgaggacg ggaaccaccg tcgagccgaa cgagagtacg atgtggcgga
     3181 cgtcgctctc ggtctcggcg ccggtgatac ggaaattgcc ctggacggtg gcgacggccg
     3241 gcttgtcgcg gcgatagagg ttgaccgtcg gcttggaggc cgacgcggga gcgaccgcct
     3301 taccgcccgt gccggcgtgg gccgaggcga gtagcttctc gacctcgtcc tcgacggcct
     3361 gttgcgcatc ggccgagcct tcgccgaggt cctcctgctt cggcaactca cgccagccga
     3421 gctgctcctc gagcgagtag gccttctcgg gtcgaacgac gcgccagttg tcgatggagc
     3481 cagtcggaca gggcgcgatg cagtccatgc agtagttgca cttctcggca tcgaccacgt
     3541 aattgttgtc gtcgtgcgtc actgcgccga cggggcaggt cgcttcgcag gtgttgcagc
     3601 ggatgcagac ctcgggatcg atgaggtgct gcttcttgag cgtggcagtt gccgtcatag
     3661 gcccaactct cgggtgctga tgtgctgttc ggggcgcaca cccgtcggtg aacgcgaatt
     3721 ggatcgctgc cgcacgagcc gctccgggac gatatcgagc cgcatgccac cacgcggcca
     3781 gagtgtcacg cgtccgagcg gctcgggcgn nnnnnnnnnc tcgcggccat tccaagcgaa
     3841 gcgcgcgccg cgcagcatgg cggcgagcat gacgacgccc tcgaccatcg cgaacgtcgc
     3901 gccgatgcag atgcgcggcc caaagccgaa cggcatgtac tgcgtccgcc gatagctcgc
     3961 gcgcttctcg gggaggaagc gcatcggatc gaagcgctcg ggctcgtccc agaggcgcct
     4021 atggcgatgg atagcccaga tcgggatgaa gaccagcgta tcggcgtcga tcgtatgcgc
     4081 gccgagggtg atcgatctgg ttgcgatacg cgtgagcacc ggggctggcg catagaggcg
     4141 catcgcctcc tcgaggatca tgcgcgtcag cgacagcttc tcaatgtgtt cggccgcgat
     4201 cggcgcgtcg ccgacgacct cggcgatttc gcgccgcgcc gcctcctgcc acacgggatg
     4261 gcgggccagc acatatagcg tccaggtgat cgccttggcc gtcgtctcgt ggccggcgtt
     4321 gaggaaggtg atcaggttgt tcaggatttg cgtctccgac atgggctcgc ccttgtcggt
     4381 cgccgtgacg aggtgcgaga ggagatggcg cccctctccg ccttcggcct gccagcgcgt
     4441 gtagagcgcg agaatcgcgt tgcgcatgtc gacagtcgcc gcattgagtt gaaggcgacc
     4501 cggatgccag gcgtcagccg gacgacgcat cagcgccgag acgatctccc agggtgcggc
     4561 attgagatag gtcgcaaccg cgctcctgat cttcaggctc tcgtgcatcg ccatgccgcc
     4621 gaacagcgtc tccgagatcg cctcgtaggt cgcctcggtg acgtcgatgt cgacaggatg
     4681 gtcggagagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcca
     4741 ggatgtcctt cgggcggaac agcggcgcaa cggccttcct ctgccagcgc cacgcctcgc
     4801 cttgcgccgt caggatggag ttgccgagtg ggtcctggaa gacgcgggct tcgagcggcg
     4861 gcttcgggaa cacgtcgtgg ctgtcgagaa agatggcctc gatgaggtcg ggatcggtcg
     4921 cccaggccgt gcggcggtcg gccgagaaga cgatcggctc ctcgtagacc gtctcgggca
     4981 ggcagcgcag cggattgcgc aggaaatggt agaggaagag cgccggcggn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnccc gtccgcgccg ccgagggcgt cgagccaacc gagtgtcgct
     5161 tgctgggccg catcggtcat cgccggagac acacttccta cccaatgttg agcgcgcagc
     5221 gcagccggcg ccaaaccgtc atggagagtg cgcggcctcc gcccggccgc gcactcaacg
     5281 cgtcacgccc tgtggatctt tacgtactcg aagtcgccgc ccttgttgtc gatgccgacc
     5341 ttcggcgccg cgatccaggg cgcgtacttc ccgacgcccc agcacggctg catgnnnnnn
     5401 nnnncgggcc ggttggcgcg ataacggcat ggcggtcagt cgccgtccga tttcgagggc
     5461 agccacttgt cgcggttcgc cgcccactcg gtctcgctca gcacgcggcc gtcgggcgag
     5521 cacttcacgt ccttgaactc gccgatgtgg cgatggaagg cgacggacgg cagcgtcagc
     5581 gtgaacttga tgccggcctt ctcaatgatg cggttccagc gcgacacgcc gccgaggcag
     5641 tccttcgagt agtcgtcgcg gagccgcatg ttgatcgcgg tcagcgccgg ctcatcgact
     5701 gtcttcagct caccgttgac gagcttcagg acggggtagg ttgccttggt cagaagatga
     5761 tcgtcctcga tcttcgtctc catgaagcgg cccttgagac ccgcgttgta gaagttcgcg
     5821 gcgttcgtcg agatctcgga gccgaagagg tcgagcgaga gcgagtagtg gaggttcgcc
     5881 ttcttctgga tggtcggcag gtcgatgacg ccgagcgcgc ggatgcgatt gatgtcgaag
     5941 gggtcctcga tgccggcggc cttcattgcc tcgcaggtgc gctcgatggt gcgaccgacg
     6001 cccgtctcgc cgacgaacat gtggtgcgcc tcctccgtca gcatgaagcg gcaggtgcgc
     6061 gacatcggat caaagccgga ctgcgcaagg ctctcgagct gcatcttgcc gtcacggtcc
     6121 gtgaagaacg tgaacatgaa gaacgagagc cagtccggcg tctcctcgtt gaaggcgccg
     6181 agcatgcgcg gcttgtcgac gtcgcccgag cgacgctgca ggagaccttc cgcctcctcg
     6241 cggccatcct tgccgaaata cttctgcagc agatagacca tcgcccagag gtggcggccc
     6301 tcctcgacgt tcacctggaa gaggttgcgc atgtcgtaga gcgagggcgc agtcttgccg
     6361 aggaagcgct gctgctcgac tgatgccggc tccgtgtcgc cctggatgac gacgaggcgg
     6421 cggagcatcg cgcggtactc gccgggcacc tcctgccagg ccttctcgcc cttgtgggcg
     6481 ccgaagttga cgaggcggct atcgtcctgc ggcgcaagga gaatgcccca gcggtactcc
     6541 ggcatcttca cgtagtcgaa cttggcccag cccttgggat cgatcgagac ggcggtgcgc
     6601 agatagacgt ccgaggtctg gaagccttcg ggccccatgg tcatccacca gtctatgtaa
     6661 ccgggatgcc actgctcgag cgccttcagc acgcgctgat ccgaggcgag gttcacgttg
     6721 ttcgggatct tcgtcgtgta gtcgacggcg ccggcaggcg tggcagatgt ggtcattggg
     6781 ttttcccttt ttttcaagga ggcaatgggc agtaggcaat aatttgcccc gcctaccgat
     6841 ctacggcccg cataaggctg cgatgcatct tgccgatctc ctcggaagcg ctcaactgac
     6901 ttgaaaggac gtcctggtcc agaagcccga cgcgatgggc caattcaatg tgcgtttcga
     6961 atgccttcaa cgattcatgc gcaatgcgca gctgctgcgc gtaggctccc gggctctcgc
     7021 gccgatagcc ttcggcaaca ttggcaggaa tggaagcaca actgcggcaa atcggactgg
     7081 tcaggccgtc ccgctcgtcg acagggaacg ccgcagtcaa ccgataggca cccacggcca
     7141 aatccatgct cttctgccaa accaggagat cacgatggct cttaattgtt ccactcacga
     7201 taccaatccc gcctattgcc tattgcctca cgtctcacac tcgctccgga ttgtagtcgc
     7261 ccttcacgcc gctgccgtag cggcgcagcg cgccgttctc gccggtggcg ctcgggcgat
     7321 tgaagatcca gttctgccac gccgtcaggc ggccgaagat cttggtctcc atggtctcgg
     7381 gaccgccgaa gcgcaggttc gcctcgaggc cggtcaggcc gtcggccgaa aagctcgaac
     7441 gctcctcgag gaagatgcgc acctcatcgg catagtcgat ttcgtcgaag gcctcagtaa
     7501 cgagaccgag ggaggctgcg tccgagccgt cgagttcctg gccgactgcg gccttggcct
     7561 tccccacgct ctccggctcg ccgaggaagc gcgtctcgag gcgcgtcagc ccgttagaca
     7621 tcgggtagcg tccaaagttc agtgccgaga gcatcatgcg cgcagccgga cgattgtcgc
     7681 ccttgaaggt gccgtcgagc atataggcgc gatcggacgc ccaggcgatc tcggcgagtg
     7741 tgccggcaaa acacgagccc ggctcgatga gcgtgacgag cgagcgcgag gtcacgtcga
     7801 tgcgcttcag cacgcgcttc cagttgttga ggatttcgcg cacgagccag tcggatgcat
     7861 tggcatgcat caccgcgtca taggcgagaa ccttcgccgg ctcaccttcc gacttgaaga
     7921 cgagcgtgcc gatctcgagc tcgttcaaac gcagatgcag gatcgcgtcg tcgagctcgc
     7981 gggcgagctt cagcatgtag aagttggcgc cttgcgcgtg aatggccgcc gcatcggccg
     8041 gcggcgcctc cttcgggccg ctgatcatga ccgtggcgcg gcgccccgcg cggtcgatct
     8101 cgactctcac ggtctcgtag gcaatctggt ccttcgacag cgtgcgcgtc aacggggtga
     8161 gcgcaatgcc cttggcgtcc gcggggcgat cggacttggc cgcgagcttc ttcgccgcct
     8221 cggtgacggc ggcgtcgaac ttggagttcg gcaccgtctg gtcgacgagg cgccatttca
     8281 cggcgcgcgc gcccttgatg ccttcctcga agcccgggat gaaaccggca ttgccgatca
     8341 ctcgacggcg cgtttcccct tgatgccctc ctcgatggtg cagaaggcgt cggcgcggtc
     8401 gcggcgcacc ttgcgcttgt cggtgacgcg cgtgaggccg cctgtgccag gaagaacggc
     8461 gagcagcggg atctcgggga gcgacacggt cgagttgccg tcatcggcca gaatgatctc
     8521 gtcgcaggag agcgcgagct cgtagccgcc gccggccgcc gtgccgcgca ccgcgcagag
     8581 gaacttctgg ccggagtgct cggagctgtc ctccatgccg ttgcgcgtct cgttcgtaaa
     8641 cttgcagaag ttgaccttat gggcgtgcgc cgagcccgcc aacatgcgga tgttggcgcc
     8701 ggcgcagaag accttgtcct tgcccgactt cagcacgacg accttcaccg ctggatactc
     8761 gaagcgcagc cgctgcagcg catccgcaag ctcgatgtcg acgccgagat cgtaggaatt
     8821 gagcttcagc tggtatccct ggaagagcgt ggcgtcctgg tcgacgtcca tgacgagttc
     8881 ggcaacgtcg ccggcgatcc tgagcttcca gtggcgatag gtgtccggtg tccgcgcaaa
     8941 gtcgatgatg cccgactcgg ccgtcagttt ggccacatgc tctgccattg cgcgctcccg
     9001 tagtgcataa ttgatcattg attgacgtca tatagatgca atttattgca gttgtcaata
     9061 ggattcattg ccataaccgg aataaaaatg cctcgacttg acctggacct ccaaatactg
     9121 cattatattg cctgttgcgc cacgggcgtc cgataacgtc gaggccgagc aatggacact
     9181 gcacccatca cacgacggat cggcacgcgg gtgcgagccg gccgtgcccg catcggcatg
     9241 acgcgcaagc agctcgccgc agcggccgag gtctcggagc gatacctgaa cgaactcgaa
     9301 aacggcgacg ccaatgcttc tgtcgccatc cttgcaaagg tcgcagacgc gctcggcctc
     9361 gatctcgtga gcctcctcag cgccgatgat gcgtctccag gagttgcagc gcaacccgct
     9421 ttcgagccgc tcgcaagcct catggccggg atgtcgctcg ccgagcagca ggccgcacta
     9481 ccggtcctgg acacattcat cacggaccgc cgccggcggc tgcgcggcat cgccttgctc
     9541 ggactgcggg gtgcaggcaa atccaccatc ggacggctgt tcgccgcgcg tcatgggctc
     9601 ggcttcatct cggtcacgcg cgagatcgag gcgcgcgccg gcatgagcct cgccgagctc
     9661 ttcaatctcg gcgggccgga tgcctaccgc tcgctcgaga atgaggtcat cgcggagctg
     9721 acgcggcgtc ccgaccgact ggttctggag acggccggcg gcatcgtcgg caacaatcag
     9781 gcgctggaca tgatcctcgg cgccttcaag accgtttggc tcaaggcgac cccggaggag
     9841 catctcgcac gcgtttccgg acagggtgac acgcggccga tgcgcggtca tccgaaagca
     9901 ctcgagcatc tgcgggcact tctcgccgct cgcgagcccg actacgcacg cgccgagagc
     9961 gtgatcgata cctcgggccg cacggtcgaa gactgcgtcg ccgacctcga gcgcatcgcc
    10021 gggggcgtgg tcacgcaaga cgctgcttga gtgcgcctcg ccagaaccag acctcggccg
    10081 acccgttcgg cgcgaaaatg aggcggccac cgcattggtg ccgcctcgct caactcaccg
    10141 cataccgacg tcagttacgg caagcgcttc aattcttgct cttgtcgacg agcgcctttt
    10201 ccttgatcca gggcatcatg gcgcgcagct tggtgccgat ctcctcgatc gggtgggcgt
    10261 cgttgaggcg acgcgtcgcc ttgaacttcg gctggcccgc cttgcactcg cggatccact
    10321 cgctcgtgaa cttgccctgc tggatgtcgg cgagcacgcg gcgcatctcg gcgcgcgtct
    10381 cgtcggtgat gatgcgcggc cccgtcacgt actcgccgaa ctcggccgtg ttcgagatcg
    10441 agtagttcat ggtcgcgatg ccgccttcgt agatgaggtc gacaatcagc ttcacctcgt
    10501 gcaggcactc gaaatacgcc atctcggggg cgtaacccgc ctcgaccagc gtttcgtagc
    10561 cggcgcggat gagttcgacc agaccgccgc agagcacgac ctgctcgccg aagaggtcgg
    10621 tctcgcactc ctccttgaag ctcgtctcga tgacgccggc gcggccgccg ccgatcgctg
    10681 ccgcatagga gaggaagagg tcgtgcgcgt tgccgctctt gtcctggtgg atggcgagga
    10741 ggcagggaac gccgccgccg cgctgatact cggagcgcac tgtgtggccc ggacccttcg
    10801 gcgcgaccat gccgacgtcg aggtcggcgc gcggctcgat caggttgnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnntgag gccgtgggcg aacatgatcg ccgcgccctt cttcatgttc
    10921 tcgtgcaggt ggtccttgta gatgtcggcc tggagctcgt ccggcgtgca catcatcatc
    10981 acgtcggccc acttggcggc cgctgcgacc tccatcacct tgaagcctgc gccctcggcc
    11041 ttcttgacgc cctgcgagcc cttgcggagc gcaatcacca catctttgac gcctgagtcg
    11101 cgcaggttct gcgcatgggc atgaccctgg ctgccgtaac cgatgacggc gaccttcttg
    11161 ctcttgatca gattgatgtc ggcgtcacgg tcgtaataca cgcgcatggn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcggcccg gcatgcggct
    11341 ggcgccggtc tcacggcctg caggctcagg tgccgcgcat ggcgcggacc gagcgccaga
    11401 gcgcaccgag cgagccggtg gcgaggccga tcgtcaggca gacaccgact tcgagcgtgg
    11461 aaatgtcgta cttgcggtag tcctcgatcg ggaaaacctg gtcgagggtc gcgaacgcgg
    11521 cccggctcag caggaggccc catacgcccc accagcagac accagcgagg acatcccaca
    11581 cccgctcgcc cgccgtcggc ttcgtcggct catccgcctg gggcgtcgtg gccttgagcc
    11641 actcctgcca ggtcggcagc gtcgtcgtgc cgacctctgg cttgatcggc gggacgtaga
    11701 ctggcggcga cgccggcggc ggcagcgctc ggaactggcg ccacgtctcg ggcaccatgc
    11761 gcaagggctc gtagggcgag cgaaagcccg cgacgtattc gatcacccgg ggatcgatcg
    11821 gaatgccgcg ggcgcggtcc ttctcgaccc aggtcccgac ataggcccga ccccattccg
    11881 ttctctgctt cggcacgatc ataccgtact cgaccaaggc ctgatgagca tagctctcgg
    11941 cttccatctc ctgcacgtgg ctcggcaaca ggaggccggg cccactgctc tgcaggaaga
    12001 tatggccgca ctcgtgggcg accgtgatca agtgccggat gtttcggccc tcgggcgagt
    12061 agatgttgcc cgttccgagc caggcaagac cgtcgaggcc cttctcgttg aggtgatgcg
    12121 tcgtgacgcc cgcctcgcgc ttcatggcat ccgcgatcga gnnnnnnnnn ncggcgatcc
    12181 gcgccttgat ctcctcaccc ttgctcgctt ccaccgcaag aatatgcgtc ttcgacgacg
    12241 ccttcgctga agcgggcatc cggtacactc tcgaagctgt ttggaacccc tcgaccgttg
    12301 agagaaatat aggtttcgag cgcgcttctt caagctcggc ggtacgattt tcaacctcga
    12361 aaaagggtca gatcacgaca cggattttcg gctccaagac cgagatcacg cgcgcgagtt
    12421 cgtgaccgcg cttgaggatt tgcccgccgg ccgcgacgac actgtaggcg ccctgcttgc
    12481 gcgcgagctt cgggcatttc tcgatgcgat agagcggcat ctcgctcgag cggcggaaga
    12541 cgctgaacac ggccttctcg cgatcgaagt ccatggcgta gtcgcgccat tcacctgccg
    12601 cgacctgccg gccatataga tcgaggatct gcgtgagctc ggcccggtcg aacgcgcaca
    12661 ccgcctcttt cccggcggtc gcgctgacca cgcttgcacc ctgcccccag cccgcgccgc
    12721 ctgatccggc gctggcacga gggctgaacg gtatcggctc agagtcgctc aacgacgcct
    12781 ccctgctcgc ggtcactgca tctttgcgtg aggcggcgcg cttgacaagc cggtcacgga
    12841 aaaatcagtg cgccgcgagc gcgcgcgcag cagccgcgac acccgcgccc gcttccaacg
    12901 ccaggccgag cgaggcgagc acgtcctcga gatgcgcgag atcggtgagc acgtcgctgt
    12961 ccgtcacggc gcccatggtg ccgatgcgga tcaccttgcc ggaaagccga ttgcgcgcgc
    13021 ccgcgatcac cgagcgatgg cgctcgtaca tgccgcgcac aatcgcccca ccgtcatagc
    13081 ccttgggtgc gtggaacacc gcgaccgtcg tcgactgatg ccgcgagcgc gtgaaattct
    13141 cgaggccgat cgcggcagca cccgctttca aggcccgcga cagccgcgcg tgccgcgcga
    13201 acacccgctc gaggccctcc tcgtgcatca tcgccagcgc ttcgttgagc gcataaacga
    13261 ggctcaccgg tcctgtgaag gtcgtgtcac ccttctcggc cgaatcacgc gccttgcgga
    13321 aatcgaagta gaagcgcggc agccggtcgt cgcggccgac caccgtccat gccttttctg
    13381 agacgctcgc gagccctatg ccgggtggcg acattagcgc cttctgcgag ccggtgacga
    13441 gtacatcgac gcctgccgcg tcctgcccga tcggcatgcc gccgaggccc gagacactgt
    13501 cgacgacgag gagtgccggt gtcttcgcaa cgacacggcc gacagcggcg agatcggcgg
    13561 cgacacccgt cgagctctcg ttgtgcacca tcacgacggc gcggtagtcg gcgctcgcga
    13621 gccgcttggc aacgtcggcc ggatcggcct cctcgcccca cggcaccacg agcggatcaa
    13681 ccacgacgcc gtaggccttg gcgatctggg aaaagcgctc gccgaactgg ccgttctcga
    13741 tgacgaggat gcgctcgccc gccgcaagca cgttgacgag cgccgcctcc atcatgcccg
    13801 tgccgctcga cgaatgcagg aggatgcggt tccgcgtacc gaatacggga gcgagccgct
    13861 cgaccgccgc ggcaaggatc gccttgaact cagcgccgcg atgcgcctgc accgggcgcg
    13921 ccatggcgag ccggacgcgc tcgggtaccg ccgtcggccc cgggatgcgc aggcgatagg
    13981 tctcgggcgg ggccatgttg gcgaggcggg gcgtggtcat gtcgggctct cggcgtgcgt
    14041 tgaggtcgtg aggaaaccat gcggccgcac ggctcggcaa gcgcaacgcc tgtcgatgag
    14101 gtgtgcgccg gcgggaggtc ccagatccga cggacgaccg tcgaagtgtt cagaactctg
    14161 ccccgagccc cgccaaagcc cgtcgtcgcg gagcgatgct tggcatcgac ggcctctgga
    14221 atccccacgt ttcttggtcc tcagatcagg cgagcaccgg gcgcgggcgc gtgatcattg
    14281 atcaaaccga tcggatcatc cggcgtcggc aaattccggt tctgtcggct gcaatcgtaa
    14341 acaacaccca aatcgcacac acagcgctcc ggtagcgagc catcacaggt acaggatcca
    14401 tgcacgctca ggatgtgacg ctcgcgctct tcacggcggt caacctgatc cgcgtgtttt
    14461 catacctccc ccagatccgc tgcatcgccc gcgacacgac cggcgccacc tgcatctcct
    14521 acaccacctg ggccatgtgg gtgctcggcc acgccacgac gctgctctac gcgatcgtca
    14581 acctaggcga ccccgtcctc gccaccgtca gcaccatcaa catgacttgc tgcctcaccg
    14641 tgatcgccct caccgcctgg aagcgccggg tggttgccat ggccggaagc atcgcggcgt
    14701 gagtgcgttg tcaaagctcg tcgacgagcg ccgccgtaac agggatggga cttcgagagc
    14761 cgtctgcgcc accgcgcgaa tcgagcacgt gaacctgcct caaatcttgg ccggccaccc
    14821 ggcggcgatc gatttccgga acatcgccgg tccgggtagg caagcggcgg ctcgcgtggc
    14881 actctttggc ggcgaaggag catccggggg ctcagatgat cgagacgccc gagcgcgcgc
    14941 tcgcctctgt cggcctgttc aagggtctgt cgcgggaggc gcttgcgaca ctcacccgcc
    15001 gcgccgtcat gcgcgaggtg ccgcgtggtc atgtcgtcgt ccggcagggc gaggcggccg
    15061 acgcgctgca catcgtcgtc tcgggccgct tcgacgtgac gctcgaaggc cggccgcgcc
    15121 cgatcgccga gatcacggcg ggctccccca tcggcgaaat cgccttcctc gccggcggca
    15181 cgcgcacggc gaccgtcact gcggtccgcg acggcatcgt catgacgctc gggcgcgccg
    15241 acttcgacga gctcctgaag accaacccgg acgccgcgcg catcatgatg gcgactgtcg
    15301 ccgggcgact cgcggctgcg accgcgacca tggcaccgcg ggccgccgca cgaccgaaaa
    15361 cgatcgccat cattacggcg ggcgggcggc cggtgcccat ggcgtttctg cagaagctcc
    15421 gcgaggtcgt cgatcggcgc tccagatccc tgatcgtcgg ctctgagacc gtcggccggg
    15481 tcctcgacgg cattcgcggc gatgccgaga tgacgaccgc cctcaacgcc atcgaggccg
    15541 actacgattt cgtcgtctat ctcgccgacc cggcactcac gccctggtcc gagaaggcga
    15601 tccggcaggc cgatctcgta ctgcggattg ccgatgtgcg cacctcgcac ggggcccttg
    15661 agtgcggcga gaacgatctc gagcgctttg cgacgcgtct ccaccagtct gctggacaac
    15721 gcctcgtgct cgtccacgaa acgcagagtg ctgtctcggg gactcgggcc tggctgcaag
    15781 accggtcggt tgcgcagcac catcacgtgg ctctcgacga cgctgcaagc ttcgagcggc
    15841 tcttccgctt catcgccggc acggcgcgcg gcctcgtcgc ctgcgggggg gctgcgttct
    15901 gcgccgccca catcggcatc tatcaggcgc tcggcgaggc cggcgtcgag atcgacatgc
    15961 tcggcggcac gtccggcggt gccgcgatga cggcggggtt cgccgccggc ttcaccccgg
    16021 ccgcgatgag cgaggccgtg cgcgacatct tcgtcaccaa ccgtgccatg cgccgctaca
    16081 cctggccgcg ctacagcctg ctcgatcaca cccatttcga ccgcatgctc gagcgctact
    16141 acggccgcat cgacatcgag gatctgtggc tcccctactt cgccgtctcg acgaacctct
    16201 cgcgttacgc gctccactgc cacgcctcgg gtgacctctt ccatgccgtg cgcgcctcgg
    16261 cctcgatccc ggcgctcctg ccgccgctct acaccgagga cgggcagatg ctcgtcgacg
    16321 gctgcctcct cgacaatgtc ccgatccgca tgatgcgcga gctgaagagc ggccccaaca
    16381 tcgtcgtcgc cttcgaggtg cccgagctgc agcgcttcaa ggtcgattat gcgcgcctgc
    16441 cgagccgcgc cgaactcttg aagcgcgccg ccatgccctg gcgccgcgag gcgctccccg
    16501 atgcgccggg cgtcgcctcc gtgctgatgc gcagcctgat ggccaaccgg caggacttcg
    16561 agcgccacct gaagcccgac gacctgctga tcgtcccgcc gctccccgac gacatgagta
    16621 ttctcgactg gcatcgccac ggcgagcttg tgcagtccgc ctacggctgg atgcgcgatg
    16681 agatcctccg ccagcgcgcg cgtggggcga cagcgctcgg tggcggggct tcacgcctcc
    16741 cctgatctcg actaacgata gcggacggca agaaggaagc gtccatggac ttcgatctca
    16801 gcgaggagca gcagctcctc aaggacagca tcgaccggct gctcgcgagc cgctatcccg
    16861 acgtgaagac gcgggccggc cacatggctg ctccaaaagg ctactccgcc gcgatctggc
    16921 aggagatcgc cgacctcggc ctcctcggca tacccttcgc cgaggcacat ggcgggctcg
    16981 gccagggcta cgtcgagatc atgatcgtca tggaggcgat cggccgaaat ctggcgctcg
    17041 agccgtatct gccgaccgtc gtgctcggcg gcggctgcct gcgccacgcg gggagtgatg
    17101 cgcagatcgg ggcgctcgcg cctgacatca tcgccggcaa gacgacactc gcgctcgccc
    17161 acctcgagaa acaggcgcgc tacgatctcg ccgacaccgc tatgtccgcc aaggcggacg
    17221 gcaaaggggg ctatgtcctc gagggcgaga agtgcgtcgt gctcggcggc gacagcgccg
    17281 acaagctcgt cgtcactgcg cgcacaagtg gtgatcggcg gagctcctcg ggtatcgggc
    17341 tcttcatggt ggatgcgacg gccggcggca tctcacggcg cggctaccag acccaggacg
    17401 ggatgagggc cgcagacatc gccttctcgg gcgtgagggt tgggaaggac ggcgttctcg
    17461 gcgatccggc cggcggctat tcggtgctcg cgcgcgtcgt cgacgaagcc atcgcggcgc
    17521 tcgccgccga ggcggtcggc gccatggagg gcgcgcatct cctcaccgtc gagtacctga
    17581 agacgcgcaa gcagttcggc gtgccgatcg gctccttcca ggtgctccag catcgctccg
    17641 tcgacatgtt cacgaacctc gagcagtcgc gctcgatggc gctcctcgcc tcgatgatgg
    17701 cggccgagcc cgatgcggcc gagcgcgccc gtgcgatgag cgcggtcaag gtgcagatcg
    17761 gccgatcggg acgattcatc ggccaggagg cgacgcagct ccacggcggc atcggcatga
    17821 ccatggagta caagagcggc cactacttca agcggctcgc catgatcgac ctcgccttcg
    17881 ggcccgccga ccaccacctg aagaaactcg ccgaggctgg cggcgtcttg agcgaggcgg
    17941 cgtgaacgag gcccctgttc gcccgccgat ccgccgcctc gtcggcacgg ttgcgctgct
    18001 cgtgttcctg gtcgtctacg cgctcgccgt gatgatggtc gcgatcacgg tgctcccaca
    18061 gctcggcaaa tgggccgagc cggtgttcta cctggtcggc gggctcctct ggatcatccc
    18121 ggcggggttc ctgatccggt ggatgtaccg gccggggacg tgacccggag gcaactccag
    18181 tggacagaga cctgtcggaa ggaaacgtga tcgtacgagc tctctgccgt cgcagcatga
    18241 cgtctcgacc taccgcatcg cccatctgag aggtatcatc atgaaactgt cgatcgccgc
    18301 ccgagccctg tcgctcgcgc tcgccaccgg acttggcctc acgctgctct cacctcccga
    18361 tgtccgggcc gaggccaaca cggtgcgcgc cgccaagcag tgggggctcg gctacctcca
    18421 gtacatgctg atggaggaca tgaagctcgt cgagaagcac gccaaggcgg ccggcctcgg
    18481 cgacgtgaag gtcgaatgga acacgttccg ctcctccgac gtcatgaacg acgcgctcat
    18541 ttccggcaac gtcgacttcg tctcgctcgg cgtccccggc ctcatgacca tctgggaccg
    18601 caccaagggc aagctcgacg tgaagggcgt gacggcgctc aactcgctgc cgctcgtcct
    18661 caacgtgcgc gcgcccgagg tgaagacgct tgccgacttc accgagaaac atcgcatcgc
    18721 ggtaccggca gtcaaggtct cagtgcaggc gatcctcctg cagatggcgg ccgagaagct
    18781 gcacggcgcg ggcaagcaca cggccctcga tgcgatcacc atcaccatgg cgcaccccga
    18841 cgcgaccgtc gccatgctga gcgcgcagac agaggtctcc gcgaactttt cctcgccccc
    18901 cttctacttc cgacagctga agcagccggg cattcgccga ctgacgtcat cgaccgagat
    18961 caacggcggg ccgctgccct tcaacgtggt agccgcgacg tcgaagttcc gctccgagaa
    19021 cccgaagctc tacaaggcct tcctcgcggc catggccgag gcgacggagc tcatcaacaa
    19081 ggacaagcgt tgggcgggtg aagcctatct gcgcattgcc aaggacaaga cgccgatcga
    19141 cgagttcatg accatcatga acgatcccga gatcaacttc acgctggcct ttgcgagctt
    19201 cgacaacatg atcaacttca tgacgcggac cggaaacttc aagaacaagc cggcgagcag
    19261 caaagagctg atgttccagg attgaagcgg caggaaccaa acccaggcga tgatcgggcc
    19321 cgagcgatgc ccgggctcga ttcattctgg aggcacagtg actctcgcca tgaccgcacg
    19381 aagccccggc actttcgtgc agaccacgtc ccatactata tcagggtcga ttccgaagta
    19441 ggcatgggcg agcatgtctc gcagacctgc gatccgacgc cactcgattt gaggacagag
    19501 caagcgcgcc tcgtccggca actgcttcac cgcttcgccg atcaactcga gatttctcag
    19561 aacggcgtcc ttggtccgat cgtccgcttc gaatgccgag cggctcatgc cgtgagtgta
    19621 gcgctcaatt tattcgcacg aggttagaat atcccgccag tacagacgcc agttacgcga
    19681 cacgaatggc ctcgcgctcg atcgcgtcct tgagctccgg acgcagggcc ttcgtcgtca
    19741 ccaagtccac acggagcccg agcaagcctt cgaggaaatc cttgaggtcg atgaagcgat
    19801 cgaatgttgt cggctcggta aactcgacaa gtatatcgat gtcacttgca ggccccggcg
    19861 ttccgcgtgc ccgagatcca aacacgagca gcctagccac accgaaatcg gagtagcggg
    19921 acctgtgcag ctcgaggatg tccactaggc tcaacggatg tctccaatgt tgatctcggt
    19981 gaactaaact tcagtctgtc gattgtttgg tgccagaatg tgccaatgat cagaaaatac
    20041 tgatatcgca ggtaaccatt ggactggtcc actcccggct acggcgcggc cgcttgacac
    20101 attctctctg cggatcaact ccgagcgctt ctgcttcgat ggcccaacga catagtcgat
    20161 tgtcttgagg accaagaagg caagaaacaa caggaccacg agtccaatga cgccgcccaa
    20221 cagatcgggc aagataaaac ctaatacgcc accaaggaac aatcctcctg caaagccaat
    20281 gaacactcct tttccgcctt cactttgccc ccgaaatagg ccccaaccgc gccacctatc
    20341 aacatgagaa tggttatcac gtggttgtga attgggcccg gttccccttt ttgccgatct
    20401 tcggtcatct ggtgcgcctc cctgtcgtcc tcttgagagg gcatatggcg cggccgagct
    20461 gaatcggccg ccccgcccca tcacttcata acgggaatgg tgaactcggc gccctccttc
    20521 acgccggacg gccagcggct cgtcacggtc ttcgttcgcg tatagaagcg cacgctgtcg
    20581 gggccatgct gattgaggtc gccgaacgcg ctcttcttcc agccgccgaa ggtgtagtag
    20641 gcgagcggca ccgggatcgg cacgttgatg ccgaccatgc cgatattgat ccgcgccgcg
    20701 aaatcgcgag ccgcatcgcc gtcgcgcgtg aagatcgcga cgccgttgcc gtactcgtgc
    20761 tccatgggga ggcgcagcgc ctcgtcgtag tccttggcgc gcaccacgga gaggacgggg
    20821 ccgaagatct cctccttgta gatgcgcatc ttcggcgtca cctcgtcgaa gaggcagccg
    20881 cccatgtaga agccgttctc gtagccctgc atgcggaagc cgcgcccgtc gaccttgagc
    20941 ttggcgcctt ccttcacgcc gatctcgacg tactgcttca ccttctcgag gtgggccttg
    21001 gtgacgaccg gaccgaagtc ggcgtcggcg ctcgtcgacg gaccgatctt caggccttcg
    21061 acgcgcggga tgagcttcga gacgagcttg tccgccgttg ccttgccgac cggcaccgcg
    21121 accgagacgg ccatgcagcg ctnnnnnnnn nnccgtaccc ggcgccgatc agcgcatcga
    21181 ccgcctggtc catgtcggcg tccggcatga tgatcatgtg gttcttggcg ccgccgaagc
    21241 actggacgcg ctttcccgca gcacacccgc gcgaatagac gtactcggcg atcggcgtcg
    21301 agccgacaaa gccgacggcc tcgatgtcgc gatcgtcgag gatggcgtcg accgcctcct
    21361 tgtcgccatt gacgacattg aggatgccgg cgggcaagcc cgcttccatc atcagctcgg
    21421 cgagcatcag gggaacgctc gggtcgcgct cggagggctt caggatgaag gcgttgccgc
    21481 aggcgatggc cggcgcgaac ttccacatcg ggatcatggc cgggaagttg aacggcgtga
    21541 tgccggcgac gaccccgagc ggctggcgca tggagtagat gtcgatgccg gggccggcgc
    21601 cttccgtgta ttcgcccttc atgagctgcg ggatgccgca ggcgaactcc gccacctcga
    21661 cgccgcgctg gatgtcgccc ttggcgtcgg cgacggtctt gccgtgctca cgggcgagcg
    21721 ccagggcgag cttctcgttg tcgcgcgcga cgagatcgac gaacttcatg aggacgcggg
    21781 cgcggcgctg cggattggtc gcggcccagg ccggctgggc agccttggcg ttctcgacgg
    21841 cggccctgac ctcggccttg gaggcgagcg gcaccttggc ggcaacctcg ccggtcatcg
    21901 gccagaacac gtcgccgaac ttgccgctcg tgcctttgac gcgcttgccg ccaatgaagt
    21961 ggtaaagttc ggttgccatg tcgcgctcct gctcgatccc ggatcggtcc gcctcgggcg
    22021 gccgtgtccg gtcatttagg ccgctcgacg ggctcgcgtc caggccgacc ggccatgcag
    22081 cggctgctcc gggttgcagc accatgcgcc agctcggtcc gcacattttc gattcgtcaa
    22141 cactttccat gtttgatgac tgaaaggttc ccgaagcccc ggagtgcgca tccccatgtg
    22201 gtccaagttc gccaagctcg ccgcagtcgc cgtggttgcc gccgtcgtca cggccgctcc
    22261 ggtcagtgcc gccgacctca tcgtcaagta tgaccagtcg cagatcatcc gcctcgaccg
    22321 cccggtcgcc gaaatcatca tcggcaactc gacgatcgcg gacgtctcgg ttcaggcccc
    22381 gcagatgctg gtcgtcaccg gcaagacctt tggcatcacg aacgtcatca tcctcgacgc
    22441 ctcgcgccag accatcgccg agcaccgcat catcgtgcag cgcgacgagg tgaacattgt
    22501 cacgctgcat gccgccgcca aacgccagtc gtacaactgc acgccgcgct gcaatccttc
    22561 gctcgtcgtc ggcgacgacc tcaactactt cgagacggtc gcgaaggtct ccgagcgcaa
    22621 gatcaagttc tcggaaggtg ccaacgacgc gagcggcggc ggccagtaac tgaccatctc
    22681 cgctgggcga gaccaggcac atcactcgcg ggtggtgtgc cttccttttt tccccacccg
    22741 caaatcgccg cgcactacag taccttgcgg cgacctgtta gaagctgcgt gtcaacggcg
    22801 actcatgacc aatccgatgc gccgtatccg ccgtatccgc cgttgcaccc gccagccgcg
    22861 cgcaatcgat caaggacgct cagcatgacc gacatcgtca tctggatgct tgccatcgcg
    22921 gtcagcgtgg ccgcgctggt cgtcgccgcc gccgccaagc tgcactacat ccacatggcc
    22981 gttgccgcag tcatggcgat cttcatcgcg ctttcggccg tgcgcgagct gcgcgtgaca
    23041 acagcgtccg gcgcaggaga cgccgccgtg gcgagcgtcg ctgcgcgcta catgggcctc
    23101 gtgtgggcct ggggcgccat cgtcctcatg atcacctaca tgttcatcct gcagtggaag
    23161 gagtggtggc agttcttcat cgccttcatc gtcgccgccg gcatctgcct cttcttctca
    23221 gcgaccctgc gcagcgacag cgccaagggc gccgatgatc ccggcatgct gagcctctcc
    23281 cgctacctcg cgctggcgca ggccatcggc accgtggtca cgatcgtcgg gctcggcgtg
    23341 ttcgacggca agggtttccg cctcggcggc aagatgctcc gcttcctcga tccccggcac
    23401 acggactggg ccgccaacaa cgtcttcttc ttcggcgcga tcgcgctcgc cgctatcagc
    23461 ttcacggcac tttccgcgct cggccgctcc aggcgataga agccgcgtga tggcgacagc
    23521 gaccgacgcc ccctctctct cccgtctcat gccgcggctt gcggccgtgc acctggcgag
    23581 cgtcgggggt atcatcgtcg gtgcgggcct cggccgcccg ctgctggtcc aggcgttcgc
    23641 cgcgctcttc gcaaccgtga tcatattggt ggccatcgcc atcaatcggc cgctgtgggg
    23701 ggcggagacg gctgccgtag cgcctgccga agcagcgcgg cgcaacatcc gcctcgtcgc
    23761 gcttgcttac gcctggggcg cgcttgcgat gcttgccgca tatgcccttt caggtgtgcg
    23821 ctggcagcac ggctggcagt acggcgccgg catggcggtg atctcggcga tcacgatgct
    23881 cgtcgcccat cggcagggcg atccgcggtc cgttctcgcg cgagccccgg cactgcgact
    23941 gctgtcgctc gtcaacgtgc tgcacggcgc tgcagcgatc gccgccctcg cctggctgat
    24001 cggctccggc aagctcatga gcccgcgcgg cgactgggtc gccaatcaga tttttctgtt
    24061 cggtggcctc gtcatcgtcg cgctctcggt gttcgcctgg ctcacgcacc agaggtggcg
    24121 ctgaccatca agcgccgtcg gtatcgccgc ggcggacctc ctgccggctc accatccgcc
    24181 cgctgaagcc gcgaaagcgg ccggacatct tgagccgctc cttctgcgcc gagagttcgc
    24241 tgcgccgcgc ctcatcaggg cggccgagaa gtcgctgcag ccgcgagagg cccgcaagcc
    24301 ccaaggcctt gcctccgaac agcagcccct tcgaggcgag catggctgcc ggtaccacgc
    24361 ctttctcctg gcgcgcgcgt gcgacgaggt tctgctgcat gccgcccgtg acgagcatct
    24421 tgttgacgat ctcctcctcg ggcggcgggc cgtcgccgta gtagttgtag atcagaggat
    24481 gatcggactg gcggatatcg acgccgagcg cgacagcccg gccgaagaag gcctgatcct
    24541 ccatgccggt cgcattgaag gccgggtcga agcgcaaacc gagcccgttg gcggccacca
    24601 accgccggtt gaacgagacg ttgccggcgc cgcacttctg caatttcgcg ccatgcggga
    24661 aacgctcgcg cacgccgctg atgcggccat tgacgacatc gcagccgaat tccacatgtg
    24721 atgcgagatg acccgcgagc cagccctgac tgagcagcat gtcgtcgtcg acaaacagca
    24781 ggatctccgc gtccgttctc tcgagcgcca ccttgatcgc ggcgttgcgc gcgttcgagt
    24841 agccgggctc gggctcgtgg ccatagtgga cgggccacgg caacgaggcg agcggcgcct
    24901 ggatgtagct accccacgcg ctctcacgat tgttgtcggc aacgacgatt gagaacgcga
    24961 gacccttcgg gatatcgagg gtcgcgatcg tggcgacgag tttcgcgaac gcctgcggac
    25021 ggcgataggt gcacaccatc agcgcagcgg aggtcggatg tgggcgcgag gcgttgggct
    25081 cgcccatcag cgaaggtccg ccatgagcga ctgatgaagg cgagaaaaat
//
LOCUS       DAORNH010000026        23769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1634_length_23769_cov_2.576832, whole genome shotgun sequence.
ACCESSION   DAORNH010000026 DAORNH010000000
VERSION     DAORNH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23769
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1634_length_23769_cov_2.576832"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /gene="carB"
                     /locus_tag="PK264_04600"
     CDS             complement(<1..1897)
                     /gene="carB"
                     /locus_tag="PK264_04600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRD75205.1"
                     /translation="MPKRTDISSILIIGAGPIVIGQACEFDYSGTQAVKALKAEGYRV
                     ILVNSNPATIMTDPELADATYIEPITPEIVAKIIAKERPDALLPTMGGQTALNTALAL
                     HKQGILSALGVEMIGAKAEAIDKAEDRLLFREAMKKIGLATPKSMMAHDRQEARAALE
                     FVGLPAIIRPSFTLGGTGGGVAYNREEFFEIIERGLDASPTRQVLVEESVLGWKEFEM
                     EVVRDTADNCIIVCSIENIDPMGVHTGDSITVAPALTLTDKEYQIMRNASLAVLREIG
                     VETGGSNVQFAVNPADGRLVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IQNEITGGATPASFEPTIDYVVTKIPRFAFEKFPGSDPTLTTSMKSVGEVMAIGRTFA
                     ESLQKALRGLETGLSGLDDIAFEGLGQGDDKNVIRAALGRPTPDRLLKVAQAMRLGVS
                     HEEIHASCRIDPWFLERLQELIDVEAEVKANGLPRHAEALRVLKALGFSDARLAKLAG
                     RSETEVASLRRGLGVLPVYKRIDTCAAEFASPTAYMYSTYESGLAGAPHCEAEPSEAK
                     KVIILGGGPNRIGQGIEFDYCCCHACFSLTAAGYETIMVNCNPETVSTDYDTSDRLYF
                     EPLTGEDV"
     gene            2008..2610
                     /locus_tag="PK264_04605"
     CDS             2008..2610
                     /locus_tag="PK264_04605"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD75206.1"
                     /translation="MNKHLPFERLRRDPTQAAEGLPRWRWSTAELERMAGLGVFSEDD
                     RFELIGGEIVPMSPKGNFHEVLRNQLSFVLARLCPPDIRVAFEPQFNLAPDTYTHPDI
                     LVHKAVEQVPFVKGDTALLVIEVAESSLSFDLKAKALLYARHGVREYWVIDAKRRITR
                     VHRDPEAGGFGHVHEAQSRERLVPALAPTLAVALDELTLD"
     assembly_gap    2717..2929
                     /estimated_length=213
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2930..4418
                     /locus_tag="PK264_04610"
     CDS             <2930..4418
                     /locus_tag="PK264_04610"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD75207.1"
                     /translation="GVGGTWYWNRYPGARFDSESYSYGYSFSKELLQEWDWQEHFAPQ
                     PETLRYLNHVADKFALRPDIEFRSRVSSAHYDAARRGWTVTLESGKVASARFLITAVG
                     PLSAPVMPRIPGVASFKGEAYHTATWPHQPVTFAGKRVAVIGTGATGVQTIQEVAKTV
                     GHLTVFQRTPNWCKPLKNRPITREEMAHIKANYDDIFRRCNETYACFLHTTDMRSVFD
                     VAPAEREAFLEKLYETPGFGMWQGNFRDMLTDREANRVVSDFVARKIRERVKNPAVAE
                     KLIPKNHGFGTRRVPMETHYYEVYNQPNVELVDIKATPIEAITERGIRTSEREFTFDM
                     IIFATGFDAVTGSFDRIDIRGAGGQSLKDKWRGGIETLLGIQVAGFPNMMMLVGPHTA
                     LGNIPRSIEHNVDWVARLIGHMTTQGLTHADARTEAVVAWTDFVREKGEGLLTNEVDS
                     WMTGVNTNIEGKSKRVLARYSGSAPDFRAWCDRVAEGGYRELHLA"
     gene            4497..4760
                     /locus_tag="PK264_04615"
     CDS             4497..4760
                     /locus_tag="PK264_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75208.1"
                     /translation="MNHKHRKVLHALFAHPVSSNINFREVVHVLESLGATIENKSGNR
                     VGVTLAGHTAAFIHVHHDLGKDEVAQIRKFLTTCGVDPQAYPV"
     gene            complement(4785..5150)
                     /locus_tag="PK264_04620"
     CDS             complement(4785..5150)
                     /locus_tag="PK264_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRD75209.1"
                     /translation="MKQRTLLLAAMSLLALATVAASHAADPAAIERGRLIAEGRCSRC
                     HAVGPSGASDQSDVIPFRRLAERFPSEMLVEALKSGVVGGHEEMPMSDLGLADVRALL
                     AYLDSLAPAGSRYLKDLPR"
     gene            5299..5673
                     /locus_tag="PK264_04625"
     CDS             5299..5673
                     /locus_tag="PK264_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75210.1"
                     /translation="MTPPLKLGIYLTLAAASLALAVPPRVEAQAVDCLKPVEGSASMV
                     VRQPPDAGSLNKSPDYPLAKLKARRDVGGAWMKAVAEHCPGFDTSWARAKRKNVKCGF
                     FSSTPRYSCIQTGIPARKRKSS"
     gene            5736..6689
                     /gene="prmB"
                     /locus_tag="PK264_04630"
     CDS             5736..6689
                     /gene="prmB"
                     /locus_tag="PK264_04630"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRD75211.1"
                     /translation="MRPPDEDAVSSLLTVRDWLRHATSRFRAEGLQFGHGTTRAVDEA
                     AFLILHTLSLPIDELDPWLDARLTITERRAIKDIIERRISTRKPAPYITNQAWIGGVP
                     FYVDERVIVPRPLIGELLVSGALDAALGRQAPVASILDLCTGSGCLAILAAMVWPNAS
                     VDAVDIDEGALEVARRNVTNHDLESRVRLVKSDLFAGVDGRRYDLVIANPPYVNAAAI
                     AAFPPEHRAEPILAHAGGEDGLDIVHRIMAEAPVHLAPEGQLVLEVGTARELMETAYP
                     RLAFVWLDTEETTGEVLLIAREGMLTLPPKARSGRGSRKGG"
     gene            6814..7083
                     /gene="rpsU"
                     /locus_tag="PK264_04635"
                     /pseudo
     CDS             6814..7083
                     /gene="rpsU"
                     /locus_tag="PK264_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
     assembly_gap    7149..7175
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7221..>7540
                     /locus_tag="PK264_04640"
     CDS             7221..>7540
                     /locus_tag="PK264_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75212.1"
                     /translation="MGLQTRWPRSPAHKAIVALTIQRWQGLVSADGLKGMDRMSLDDV
                     AKIVVAFVKEHRNLAAPIVFALAFGESLAFLSLIIPATIILVAISGLLGAGGYTIWNL
                     WDVLL"
     assembly_gap    7541..7550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7551..7870
                     /locus_tag="PK264_04645"
     CDS             <7551..7870
                     /locus_tag="PK264_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRD75213.1"
                     /translation="GALGYALSYWIGLYYKERVKTIWPFSSNPVLLTKGQQFFDKWGA
                     FGVFLGHFFGPVRAVIPVVAGMCAMRQLPFQIANISSAFLWAVGVMAPGMIGAEWIMK
                     WLH"
     gene            complement(7892..9181)
                     /locus_tag="PK264_04650"
     CDS             complement(7892..9181)
                     /locus_tag="PK264_04650"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD75214.1"
                     /translation="MAETLLIVFVIFILLGMPIGIALAVGVLGAAAFFPALNPIILPG
                     RFVGLLSDSFLLLSAPLFILAGNIAARGGVARVIIDLATAVVGRFRGGLAYVNVADSM
                     VFGGISGSAVADVSALGTFLIPQMERKGYDKDFATALTISTAVVAPIIPPSIIAVIFA
                     WMADQSVAAMFAAGVIPGLLVGLGMAVPVYMIAKKRNYPKEPPPTLHEFTWAVLRALP
                     ALMIPAIIMGGILFGWFTPTEAAAVAVVYALIVPPIFYREPRLRDIPQIFVDSARLSG
                     VIGMIIGFVGAFGWVLTYSKFPFKVAALIASLGPSWWLFLLLCVALYLMLGTFLTPSE
                     IILVTVPVLLPVSEAVGVHPIHFGMVCVIASAIGHITPPVGLCLFVGMSISGLSMDKL
                     VKPLLPFLAAIVATLILVLAFPAITLWLPRFLGLLKS"
     assembly_gap    9271..9332
                     /estimated_length=62
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9333..9761)
                     /locus_tag="PK264_04655"
     CDS             complement(<9333..9761)
                     /locus_tag="PK264_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD75215.1"
                     /translation="MAGAPGAGSPPSGGGPTALDRLYDVVGYIAAVVLAIMAAAVFAS
                     VVGRWLGKSFDGIDEVPRFLFVWLVALGGAAAMCRHEHTVLDYFVNLAPVGVRHVIAL
                     IVNGVMIGLFAYLVVLSATLVPNSQFQTSAGLSIPLGFVYT"
     gene            complement(9764..10780)
                     /locus_tag="PK264_04660"
     CDS             complement(9764..10780)
                     /locus_tag="PK264_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD75216.1"
                     /translation="MAGQQRSGRTVGAFTALAVAALAATFAGGAVGQVREMTVGNVNP
                     PKHGTSQTTQAFIDKLAELSGGKVKLIHHHSGALGGEREVAQQIQLGAVDFGPITTAP
                     LSTLIPEMSVFQLPYIFRDYDHVFKALDGSDTLTKYYSDVLDKKGLKLVGFIAAGYRG
                     IYGHYPINSLADVKGKKIRVQEDKILVATFKALGMISTPIAFPEVATSLQTKVIDFAE
                     GGVNTFYHTKFYDVVKNVADVRHTHQAVALIMSKASWAKLDAAGQKAVMDAWAHARAF
                     NRKFILDEDKSIQDLVKAKGVTITKPDATPFREATRSVYEEFYATPAGKDAKKMVDFI
                     LSIK"
     gene            complement(10942..11397)
                     /locus_tag="PK264_04665"
     CDS             complement(10942..11397)
                     /locus_tag="PK264_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75217.1"
                     /translation="MAPIKARGLNHISLTVKEIKPLLPFFMDGLGYDLLSLAPRDPDL
                     MSRMTGIEGVIVTIAFLEGYGHRLELIQYYAPYDRKRVAARLCDMGANHLGYDVDSME
                     EALGMAAHHGFAPTGEVIAINAGPNQGRRVAYVQNSDGVTVEFLELRKL"
     gene            complement(11521..11745)
                     /locus_tag="PK264_04670"
     CDS             complement(11521..11745)
                     /locus_tag="PK264_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HRD75218.1"
                     /translation="MPDDPADGNNDVDASSPPRVLSEAAKRALAEAEERRKRAGIGTG
                     EGRPAELGGQPGPDPTRYGDWEKGGIASDF"
     gene            11871..12830
                     /gene="htpX"
                     /locus_tag="PK264_04675"
     CDS             11871..12830
                     /gene="htpX"
                     /locus_tag="PK264_04675"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRD75219.1"
                     /translation="MPLNYAKTGLLLAVLMGIFLLMGSVIGGQTGLVVAFVLALGMNA
                     FSLWKSDKIVLRMHGAQEVDERSAPDYYGLVRELARRAELPMPKVYVMHNPQPNAFAT
                     GRSPASSAVCASTGLLEMLSREELAGVMAHELAHIKNRDTLTMTMAATIGGAISMVAQ
                     WMQFSVLFGRGGGREGGNRLLQFVAMLLAPFAAMIVQMAISRSREYQADRLGAVICGN
                     PIWLASALHKIHNFARQIRNPSAEAVPATAHLFIINPLSGRGFDNWFSTHPSTENRIV
                     ELQKLAAEMGVSGGAPAADVATAERPSILRPSRRTSDNRGPWG"
     gene            12827..14305
                     /locus_tag="PK264_04680"
     CDS             12827..14305
                     /locus_tag="PK264_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRD75220.1"
                     /translation="MKERQGRSGGSRPPSSKAAPRAPHRGKPHDRPAAERKVRENDDP
                     LAAGLAARIAAAAICESVVARGQSFDDAADASPRLAVLEPRDRAFAHLIALTVLRRLS
                     SLDAAINRHLKSELPRDAAHARTILRVAAAQIIYLATPPHAAISLAVDQCRLDRRSSR
                     FRGLANAVLRRIAERVEAGLEPSGPVHRDIPVWLMARWRAAYGDATAEAIAAASLVEA
                     PLDLTVRSDPPKWAERLSGTVLDTGSVRLAEARRVDTLDGFAEGAWWVQDAAAALPPR
                     LLGDVKGRRVADLCAAPGGKTVLLAAAGARVTAVEASESRARLIRENLARLKLDADVI
                     VADVLSLEMSETFDAVLLDAPCTATGTIRRHPDILHLKRPSDIAKLAELQGRLLDKAW
                     RLLKRGGVLVYCTCSLESEEGVQQIERLLASRAPVAIEPIAAGEAGIKAPWLTPAGYL
                     RTLPFHTPERVRSDESITLPFSGNGMDGFFAARLRRLGIPSP"
     gene            14538..14939
                     /locus_tag="PK264_04685"
     CDS             14538..14939
                     /locus_tag="PK264_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HRD75221.1"
                     /translation="MDAQPRRELIELTSDIVASYVAKNPVGPTDLPGLIQNVHDALKR
                     AQNGAAEPIRDELRPAVPVKRSVTPDHIVCLEDGQKFKSLKRHLRTRYNMSPEEYREK
                     WGLPHDYPMVAPNYAAARSELAKRMGLGTRK"
     assembly_gap    14962..15014
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15356..15371
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15372..15602)
                     /gene="gatC"
                     /locus_tag="PK264_04690"
     CDS             complement(<15372..15602)
                     /gene="gatC"
                     /locus_tag="PK264_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRD75222.1"
                     /translation="MDVDEKTVRRIARLARIRISDADVPKLRGELVGILDWVKQLDEV
                     DTDGIEPMTRVVDMKLFTRADVVTDGEKAADVT"
     gene            complement(15682..>16042)
                     /locus_tag="PK264_04695"
     CDS             complement(15682..>16042)
                     /locus_tag="PK264_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20 peptidase family dipeptidase"
                     /protein_id="HRD75223.1"
                     /translation="NHGFGMVEVSGPPNGASELFTASRTDHNHPFALWMRDAVSRAAG
                     DRCRVLPNGGGSNMTWIMQYLVGMPCAWLPLSYAGCSQHAPNEHLLLPLTRTGLGIVT
                     SVYWDLGDGALAYRRPA"
     assembly_gap    16043..16270
                     /estimated_length=228
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16271..17088)
                     /locus_tag="PK264_04700"
     CDS             complement(<16271..17088)
                     /locus_tag="PK264_04700"
                     /inference="COORDINATES: protein motif:HMM:NF005478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD75224.1"
                     /translation="MSRSTRDEALSQALSYFDDHQGGYLADLAALVAVPTESQNPERQ
                     GDLRRYLDREMTPRFGRLGYTCRIYDNPKGGAGPVLLATRIEDPALPTIIGYGHGDVV
                     LGMAGRWAEGRDPWRVTLDGERAYGRGVVDNKGQHLAHIAAIGAVIATRGRLGFNSKF
                     LIEMGEETGSPGLDQIVAANKADFAADGFFASDGPRTEISHPNITLGNRGAATFELKV
                     RLRDGGHHSGNWGGLLANPGTILAHALASIADRRGRILVEAWRPLIPDGVREVL"
     gene            17419..17823
                     /locus_tag="PK264_04705"
     CDS             17419..17823
                     /locus_tag="PK264_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75225.1"
                     /translation="MTSMKPTMGAVCALVLAGSALGCGDSNNTKPPVAGDSPPPALIG
                     SWDLVHQDRVNDVAAQKIVVTFTPDGKHTWGPVMSKCTYRFKKPDKLIIDCTKSVAGA
                     EFEYILTSVDATDLVLTNPATNPDQVNKLKRK"
     gene            complement(17861..>18479)
                     /locus_tag="PK264_04710"
     CDS             complement(17861..>18479)
                     /locus_tag="PK264_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRD75226.1"
                     /translation="DAHRIARLTIGRELCLMTARPELDLGGVRVTPPPGTFVQAVEMA
                     EQAMRSEVLAGACRGGRFADLFAGVGTFALPLARLGRVMAIDNDRSALAAIEEAGRRN
                     PGLKPIEVRARDLMRDPLSPRELDQFDVVVLDPPRVGAKSQAEALARSKVPRVIAVSC
                     NPATLVRDLAVLAAGGYAIERATPIDQFLFTPHLEAVVVLSRRKR"
     assembly_gap    18480..18529
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18530..>18871)
                     /locus_tag="PK264_04715"
     CDS             complement(<18530..>18871)
                     /locus_tag="PK264_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75227.1"
                     /translation="DAIYADWKGGLLAAAFRRAGLPVDAIAPLVMIEARSRRRAVLTA
                     VRRDGSVAIGFHGLASHALVDLRDCAVMRPEIMAAIPGLRQILGHVLEGTTAGRVTVL
                     STVTGLDVVIG"
     assembly_gap    18872..18934
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    19228..19296
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19297..19839)
                     /locus_tag="PK264_04720"
     CDS             complement(<19297..19839)
                     /locus_tag="PK264_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRD75228.1"
                     /translation="MRLDEALVCRQLAPSRSRARALVLAGEVTVNGTVELKASRTVSA
                     AALITLAGRRGRHVSLGGDKLILALDAFGAPFDPSGRSALDIGASTGGFTDVLLSRGA
                     ARIHAVDVGRDQLHARLRADPRVVAHESTDCREITADLIPDPIDLIVADVSFISLAKA
                     LPAALALARPQACLIALVKPQ"
     gene            19999..20724
                     /locus_tag="PK264_04725"
     CDS             19999..20724
                     /locus_tag="PK264_04725"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRD75229.1"
                     /translation="MDRRQSLKLMLGAAGALAAPAIIGRAPALAQAPKAAPAFTLPPL
                     GYAYEALEPHIDAATMGFHHKNHHNAFIGILNAQVEKWPDLAKKPIAEIISNLSAVPE
                     GVRTPVRNAGGGHWNHTFFWDLMTPGGPKAPTTEVAAAITAAFGSFEKMTEAVNQAGM
                     TRFGSGWAWLVVGKDKKLAAVNTPYQDTPHMDLGVKPILGVDVWEHAYYLKHQYRRGD
                     YLKAWWNTVNWNKVAANFKGAMA"
     gene            complement(20775..>21164)
                     /locus_tag="PK264_04730"
     CDS             complement(20775..>21164)
                     /locus_tag="PK264_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRD75230.1"
                     /translation="LEAKGIPSVVQRTLIRPPLGRIGPVTPEERQGCIQYSLHYGKYE
                     EALDRESAYEMLQKKAGAASAAAEEETRQKEANANSWSNTVFGKGPRGGRSMAENVAQ
                     DVTRSIARSVFNQIKNSVIKSIFGGRR"
     assembly_gap    21165..21228
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21229..22283)
                     /locus_tag="PK264_04735"
     CDS             complement(<21229..22283)
                     /locus_tag="PK264_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRD75231.1"
                     /translation="MASQSASDGPLKDGLIFIGQSVKPEYLELKLANRHGLVTGATGT
                     GKTVTLQVLAEGFSAAGVPVFASDIKGDLSGIAAVGEPKDFLLKRAKEVGLSSYDNTA
                     FPTVFWDIFGEHGHPVRATVQEMGPLLLSRLLELNEVQEGVLNIMFRVAADEKMPLLD
                     LKDLRAMVNDVGQRSKELQSKYGNAAATSVGAIQRRLLVLEEQGANNFFGEPALDIND
                     FIRHAPDGRGIVNILSADRLMKTPRLYATFLLWMLSSLFDKLPEIGDPQKPKLVFFFD
                     EAHLLFNEAPKALLEQVERVTRLIRSKGVGVYYVTQNPRDVPNSVSAQLGNRVQHALR
                     AFTPVEQKAIRAAAETFR"
     gene            22578..22652
                     /locus_tag="PK264_04740"
     tRNA            22578..22652
                     /locus_tag="PK264_04740"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22610..22612,aa:Asn,seq:gtt)
     gene            22883..23725
                     /locus_tag="PK264_04745"
     CDS             22883..23725
                     /locus_tag="PK264_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRD75232.1"
                     /translation="MSDHTHHHDCCAHDHHHGHALPAEPATNALLSARRLTLKRGQRV
                     VLDSVDIDIRAGEIVTLIGPNGAGKTTLVRVLLGLETADSGLIRRKPDLVIGYAPQRF
                     DLDPAIPMTVARFLATAATRFRDARDVPVAALLAEVGASALAERQIARLSGGELQRVI
                     LAHALARKPELLVLDEPVRGVDYLGEADLYMLIGRIRSERGLGVLLVSHDLHVVMANS
                     DRVICLNRHVCCQGIPHTVAQHPEYVRLFGPEAARALAVYHHHHDHVHDLAGAAMAAA
                     KDER"
BASE COUNT         4049 a         7721 c         7357 g         3787 t
ORIGIN      
        1 gcacatcttc gcccgtcagc ggctcgaaat agaggcggtc cgaggtgtcg tagtcggtcg
       61 aaacggtctc cgggttgcag ttgaccatga tggtctcgta gcccgccgcc gtcagagaga
      121 agcaggcgtg gcagcagcaa tagtcgaact cgatgccctg cccgatgcgg ttcggcccgc
      181 cgccgaggat gatgaccttc ttggcttccg acggctcggc ctcgcagtgc ggcgctccgg
      241 cgagcccact ctcgtaggtc gagtacatgt aggccgtcgg cgaggcgaac tcggcagcac
      301 acgtgtcgat acgcttgtag acgggcagca cgccgagccc gcgacgcagg cttgccactt
      361 cggtctcgct cctccccgca agctttgcga gccgcgcatc cgagaagccg agcgccttca
      421 gcacacgcag cgcttcagcg tgccttggca ggccgttcgc cttcacctct gcttcgacgt
      481 cgatcagctc ctgaagccgc tcgaggaacc agggatcgat ccggcaagag gcgtggatct
      541 cctcgtggga gaccccgagg cgcatcgcct gcgccacctt caatagccgg tccggcgtcg
      601 gccggccgag cgccgcgcgg atgacgttct tgtcgtcgcc ctgcccgagc ccctcgaagg
      661 cgatgtcgtc gaggccgctg agcccggtct cgaggccgcg cagtgccttc tgcaggcttt
      721 ccgcgaaggt gcgcccgatc gccatcacct cgccgaccga cttcatcgaa gtcgtgagcg
      781 tcgggtccga gcccgggaac ttctcgaaag cgaagcgcgg gatcttggtg acgacgtagt
      841 cgatcgtcgg ctcgaaggag gcgggcgttg caccgccggt gatctcgttc tggatctcgt
      901 cgagcgtgta gccgacggcg agcttcgccg cgaccttggc gatcgggaat cctgtcgcct
      961 tggaggcgag cgccgacgag cgcgagacgc gcgggttcat ctcgatgacg acgaggcgtc
     1021 cgtccgccgg gttcacggcg aactgcacgt tcgagccgcc ggtctcgacg ccgatctcgc
     1081 gcagcaccgc caaggaggcg ttgcgcatga tctggtattc cttgtcggtg agcgtcagcg
     1141 cgggggcgac cgtgatcgaa tcgcccgtat gcacgcccat cggatcgatg ttctcgatcg
     1201 agcagacgat gatgcagttg tccgccgtgt cgcggaccac ctccatctcg aactccttcc
     1261 agccgagcac gctttcctcg accagcacct ggcgcgtcgg cgaggcatcc aagccacgct
     1321 cgatgatctc gaagaactcc tcgcggttgt aggcgacgcc gccgccggtg ccaccgagcg
     1381 tgaaggacgg gcggatgatc gccggcagac cgacgaactc gagggcggcg cgcgcctcct
     1441 ggcggtcgtg cgccatcatc gacttcggcg ttgcaagacc gatctttttc atcgcctcgc
     1501 ggaagaggag ccgatcctcc gccttgtcga tggcctcggc cttggcaccg atcatctcga
     1561 cgccgagtgc tgagagaatg ccctgcttgt gcagcgccag cgccgtgttg agcgcggtct
     1621 ggccgcccat cgtcggcagc agcgcgtccg ggcgctcctt ggcgatgatc ttggcgacga
     1681 tctccggcgt gatcggctcg atataggtcg cgtccgcgag ctccggatcc gtcatgatcg
     1741 tcgccggatt ggagttgacg aggatcacgc ggtagccctc ggccttcaga gccttcacgg
     1801 cctgcgtgcc ggagtaatcg aactcgcacg cctggccgat gacgatcggg ccggcgccga
     1861 tgatgaggat cgaggagatg tcggtgcgtt ttggcatgag ccgcgtctta tcgcagcgca
     1921 gcggagatgg ctataggccg ccgcaaaggc ttggtcgacg cacgcggggt cgcttgccgc
     1981 tccgccgccg catgttaaat tggcggcatg aacaaacatc tccctttcga gcgcctccgc
     2041 cgcgacccga cccaggccgc cgaaggcctg ccgcgctggc gctggagcac tgccgagctc
     2101 gagcgcatgg cgggactcgg cgttttttcc gaggacgacc ggttcgagct cattggcggg
     2161 gagattgtgc cgatgtcgcc caagggcaat ttccacgagg tgctgcgcaa ccagctttcg
     2221 ttcgtacttg cgcgcctctg cccgcccgat attcgcgtgg cctttgaacc gcagttcaac
     2281 ctggcacccg atacctacac ccatcccgac atcctcgtgc acaaggccgt ggaacaggtt
     2341 ccgttcgtga agggcgacac tgctctcctc gtgatcgagg ttgctgaaag ctctctctcg
     2401 ttcgatctca aagcaaaggc cctgctctac gcgcgccatg gtgtccgcga gtactgggtg
     2461 atcgatgcca agcggcggat tacgcgagtt caccgcgatc ctgaggccgg cggcttcggg
     2521 cacgttcacg aggcccaaag ccgggagcgt ctcgtgcccg cactcgcgcc gaccctcgct
     2581 gtcgcgctcg acgagctgac gctcgactga gctgggtggg cgtccagcat tgcgcacggg
     2641 cataggtgac ccgcgtcatc tcgcttgacc gggatcaagc gcttctcaga cgatagcgtc
     2701 aggatcgacc gcatctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcgtcggcg
     2941 gcacctggta ctggaaccgc tatccgggcg cgcgtttcga ttccgagagc tactcctacg
     3001 gctactcgtt ctcgaaggag ctgctccagg agtgggactg gcaggagcat ttcgcgccgc
     3061 agcccgagac gctccgctac ctcaaccatg tcgccgacaa gtttgcgctc cgcccggaca
     3121 tcgagttccg ctcccgcgtc agctctgccc actacgatgc cgcacgccgc ggttggaccg
     3181 tcacgcttga gagcggcaaa gtcgccagcg cccgtttcct catcaccgcc gtcggtcccc
     3241 tctccgcgcc cgtgatgccg cgcattccgg gtgtggcgag cttcaaaggc gaagcctacc
     3301 acacggcgac ctggccgcat cagcccgtca ccttcgcggg taagcgcgtc gccgtgatcg
     3361 gcacgggtgc cacgggcgtc cagaccatcc aagaggtcgc caagacggtc ggccacctca
     3421 ccgtcttcca gcgcaccccc aactggtgca agccgctgaa gaaccggccg atcacgcggg
     3481 aggagatggc gcatatcaag gcgaactacg acgacatctt tcgccgctgc aacgagacct
     3541 atgcgtgctt cctccacacg accgacatgc gcagcgtctt cgacgtcgcg ccggccgagc
     3601 gcgaggcctt cctcgaaaag ctctacgaga cgccgggctt cggcatgtgg cagggcaact
     3661 tccgcgacat gctgaccgac cgcgaggcga accgcgtcgt ctcggacttc gtcgcgagga
     3721 aaatccgcga gcgcgtcaag aaccccgcgg tcgcggagaa gctcatcccg aagaaccacg
     3781 gcttcgggac gcgccgcgtg ccgatggaga cccactacta cgaggtctac aaccagccga
     3841 acgtcgaact cgtcgacatc aaggcgacgc cgatcgaggc gatcacggag cgcggcatcc
     3901 ggacgagcga gcgcgagttc accttcgaca tgatcatctt cgcgaccggc ttcgacgccg
     3961 tgaccgggag cttcgatcgt atcgacatcc gcggcgccgg cgggcagtcg ctcaaggaca
     4021 agtggcgcgg cggcatcgag accctgctcg gcatccaggt cgcgggcttt ccgaacatga
     4081 tgatgctggt cgggccacac accgccctcg gcaacatccc gcgctccatc gagcacaatg
     4141 tcgactgggt cgcgcgcctc atcggccaca tgacgaccca gggcctcacg cacgccgacg
     4201 cgcgcacgga agccgtcgtg gcatggaccg atttcgtgcg tgagaagggc gagggcctgc
     4261 tcacgaacga ggtcgattcc tggatgaccg gcgtcaacac gaacatcgag gggaagtcca
     4321 agcgtgtcct cgcccgctac agcggcagcg cgcccgactt ccgcgcctgg tgcgaccggg
     4381 tcgcggaagg tggctaccgg gagctccatc tcgcctgatc cagccacgtt gatgcggcgc
     4441 aaggcgcgct agtgcccgca tctgcgaagc tttcctcatc aaccaggaaa gcagacatga
     4501 accacaagca tcgtaaggtc ctgcacgccc tttttgccca cccggtgagc agcaacatca
     4561 acttcaggga ggtcgtgcac gtgctcgaga gcctcggcgc gaccatcgag aacaagtcag
     4621 gaaacagggt cggcgtgacg cttgccggcc ataccgcggc gttcatccac gtccatcacg
     4681 atctcggcaa ggacgaggtc gcccagatcc gcaaattcct gaccacctgc ggcgtcgatc
     4741 cgcaggccta tccggtctga gcccggcggc actcggaggc cgcgctaccg gggcaagtcc
     4801 ttcaggtagc gcgatccggc gggcgcgagg ctgtcgaggt aggccaagag cgcgcgcacg
     4861 tcggcgaggc cgagatcgct catcggcatc tcctcgtggc cgccgaccac accggatttc
     4921 agcgcctcga cgagcatctc gctcgggaag cgctcggcga ggcggcggaa tggaatgacg
     4981 tcactctggt cgctcgcgcc ggatggtccg accgcatggc agcgcgagca gcgcccctcg
     5041 gcgatgagcc ggccacgctc gatcgcggcc ggatccgcgg catggctcgc cgcaactgtt
     5101 gcaagcgcga ggagcgacat cgcggccaat agcaaggtgc gctgcttcat ctgtcttcgg
     5161 cccttccgcc attcgtggcc cataagccgg ttcgccatgg tccgggttga cgtcgcgcaa
     5221 tgaatttgcc gcaatccaca gaagactgag gggcggtggc gcgagccgcg ccgcagagcc
     5281 aatctcggga gccttcccat gacgcccccc ctgaaactcg gcatctacct cacactagcc
     5341 gccgcctcgc tcgcactcgc ggttccgccg cgcgtcgaag cgcaggccgt cgattgcctg
     5401 aagcccgtcg aaggctcggc gagcatggtc gtccgccagc cccccgatgc cggcagcctc
     5461 aacaaatcac ccgactatcc cttggccaag ctcaaagcac gccgcgacgt cggcggcgcc
     5521 tggatgaagg ccgttgccga acattgcccg ggcttcgaca cgtcttgggc gcgggcaaag
     5581 cggaagaacg tgaagtgcgg cttcttcagc tcgaccccgc gttacagttg catccagacg
     5641 ggcatcccag cccgaaagcg taagtccagc tgaagcctcg gcggattgac ggccgatgtc
     5701 acatcgggca gagcgggccg gcacatgagg tcccaatgcg cccgcccgac gaagatgccg
     5761 tctcgagcct cctcaccgta cgcgattggc tgcgccacgc gaccagccgc tttcgcgccg
     5821 aaggcctcca gttcgggcac ggcaccacgc gtgctgtcga cgaggccgcc ttcctcatcc
     5881 tgcacacgct gtctttgccg atcgacgagc tcgatccctg gctcgacgcg cggttgacga
     5941 tcaccgagcg ccgcgccatc aaggacatca tcgagcggcg gatcagcacc cgcaagcccg
     6001 ccccctacat caccaaccag gcctggatcg gtggcgtccc cttctacgtc gacgagcggg
     6061 tcatcgtgcc gagaccgctc atcggcgagt tgctggtgag cggggcccta gatgccgcgc
     6121 tcggtcgcca ggctcctgtc gcttccatcc tcgatctctg caccgggtcg ggttgcctcg
     6181 ccatcctcgc ggccatggtt tggccgaacg cctcggtcga tgcggtggac atcgacgagg
     6241 gcgcgctcga ggtcgctcgc cgcaatgtga ccaaccacga tctcgagtcc cgtgtgcgcc
     6301 tcgtgaaatc cgacctgttc gccggagtcg acggccgccg ctatgatctc gtcatcgcga
     6361 acccgcccta tgtcaacgcc gccgccatcg cagccttccc gcctgagcac cgcgctgagc
     6421 cgatcctcgc ccacgccggc ggcgaagacg ggctcgacat tgtccaccgc atcatggctg
     6481 aggcaccggt gcatctggca cccgaaggac agctcgtgct cgaggtcgga accgcacgcg
     6541 agctcatgga gaccgcctac ccgaggctcg ccttcgtctg gctcgatacg gaggagacga
     6601 ccggtgaagt gcttctcata gcgcgcgaag gcatgctgac cttgcccccg aaagctcgat
     6661 ccggacgagg gtcacgcaag ggcggttgag cgcgctcttc ggcattggac atgttaaccg
     6721 ggctctgtta gaagccatcc aagcgatgcg ccggctccct tcgcaggaga tccggcttct
     6781 ttggccgtcc aaccatcgag gaacgtaccg accgtgcagg ttctcgttcg tgacaacaac
     6841 gtcgatcagg cgcttaaggc gctcaagaag aagatgcagc gtgagggcat cttccgggaa
     6901 atgaagctcc gcaatcatta cgagaagccc tccgagcgca aggttcgcga aaaggcagag
     6961 gctgtgcgcc gcgcgcgcaa gctcgcgagg aagcgcgccc agcgtgaggg gctactgcca
     7021 ggcaagccgg ccgtggtgaa gcccggcgct aagggtccag gtcgcggtgg tccgcgctcg
     7081 ggtggacctg gcggctcggg cggattcggt ggctcgggcg gcttcggcgg cggcggctct
     7141 ggcgggttnn nnnnnnnnnn nnnnnnnnnn nnnnncggaa acaattgggc cgtctcgttt
     7201 gatcgaggcg gcctttatgc ttgggcctgc agacccggtg gcctcgatcc ccggctcaca
     7261 aggcaatcgt cgcgttgaca atccagcgtt ggcaaggctt ggtcagcgcg gacgggctca
     7321 aggggatgga ccggatgtct ctcgacgacg tggcaaaaat tgtcgttgcg ttcgtgaagg
     7381 agcaccgcaa tctcgcggcg ccgatcgtgt tcgcgctggc cttcggcgag tcgctcgcct
     7441 tcctgtccct catcatcccg gcgaccatta tcctggtcgc gatctcgggc ctgctcggcg
     7501 cgggcggcta caccatctgg aacctctggg atgtgctcct nnnnnnnnnn gcggagcgct
     7561 cggctatgcc ctctcctact ggatcggcct ctattacaag gagcgcgtga agacgatctg
     7621 gcccttcagc tcgaacccgg tactgctgac caaaggccag caattcttcg acaaatgggg
     7681 cgcgttcggc gtgttcctcg ggcacttctt cgggcccgtc cgtgcggtca tcccggtcgt
     7741 cgccgggatg tgcgcgatgc gccagctgcc cttccagatc gccaacattt cgtcggcgtt
     7801 cctgtgggcc gtcggcgtga tggcgccggg catgatcggt gccgagtgga tcatgaagtg
     7861 gctgcactag gcagccggca ccaaccgcgc gtcaactctt cagcagaccg aggaagcgcg
     7921 gcagccagag cgtgatggcc gggaatgcca acaccaggat cagcgtcgcg acaatcgccg
     7981 ccaggaacgg cagcagcggc ttcacgagct tgtccatcga gagcccgctg atcgacatgc
     8041 cgacgaagag acataagccc acgggcggcg tgatgtgccc gatggccgag gcgatcacgc
     8101 agaccatgcc gaagtggatc ggatggacac cgaccgcctc cgatacgggc aggagcacag
     8161 ggaccgtcac caggatgatc tcggagggcg tgaggaacgt cccgagcatc aggtagagag
     8221 ccacgcacaa gagcaggaac agccaccacg acggcccgag cgaggcgatc agcgctgcca
     8281 ccttgaaggg aaacttcgag taggtgagca cccagccgaa ggcaccgaca aagccgatga
     8341 tcatgccgat cacgcccgag agcctggccg agtcgacaaa aatttgcgga atgtcgcgga
     8401 gcctcggctc gcggtagaag atcggcggca cgataagggc atacaccacg gcaacagccg
     8461 ctgcctccgt cggcgtgaac cagccgaaca ggatgccgcc catgatgatc gccgggatca
     8521 tcaaggcggg cagcgcgcgc agcacggccc aggtgaactc gtgaagcgtc ggcggcggct
     8581 ctttgggata gttgcgcttc ttggcgatca tgtagaccgg cacagccatg ccgaggccga
     8641 cgagcaggcc cgggataacg cccgcggcaa acattgcagc cactgattga tccgccatcc
     8701 aggcgaagat cacagcgatg atcgacggcg ggatgatcgg tgccacgacc gcagtcgaga
     8761 tcgtcagcgc ggtcgcgaag tccttgtcgt agcccttccg ctccatctgc gggataagga
     8821 acgtgccgag tgctgaaacg tcggcgaccg ccgagcccga aatgccgccg aacaccatgc
     8881 tgtcggcgac gttcacgtaa gcgaggccgc ccctgaaccg cccgaccacg gccgtcgcca
     8941 agtcgatgat cacgcgcgcg acgccgccgc gcgcggcgat attgcccgcg aggatgaaca
     9001 gcggcgccga gagcagcagg aagctgtcgg atagcaatcc gacgaagcgg ccagggagaa
     9061 tgatcggatt gagggcggga aagaacgccg ccgcaccaag cacgccgacc gcgagcgcga
     9121 tgccgatcgg catcccgagc agaatgaaga tgacgaagac gatcagaaga gtttcggcca
     9181 tagagccctc aggatcgtga gtgcgctgcc cgagagccag acgccgagcg caatgaagaa
     9241 gaacgccacc gcggccatgg cacccgtcga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtgtaga cgaagccgag cggaatgctg
     9361 aggcccgcgc tggtctggaa ttgcgagttc ggcaccagcg tcgcgctcag cacgacgagg
     9421 taggcgaaga gcccgatcat caccccgttg acgatgagcg cgatgacatg ccggaccccg
     9481 acaggcgcga ggttcacgaa gtagtcgagc acggtatgct cgtgccggca catggccgcg
     9541 gcgccgccga gagcgacaag ccacacgaac aggaagcgcg gcacctcgtc gatgccgtca
     9601 aaggacttgc cgagccagcg tccaaccacc gacgcaaaca cggcggcggc catgatcgca
     9661 aggaccacgg cggcgatata gccaacgacg tcataaaggc gatcgagcgc ggtgggcccg
     9721 ccacccgagg gcggcgaacc ggcgccgggg gctccggcca tggctatttg atcgacagga
     9781 tgaagtcgac catcttcttg gcgtccttgc ccgcgggcgt cgcgtagaac tcctcgtaga
     9841 cgctgcgcgt tgcttcgcgg aacggtgtcg catcaggctt ggtgatcgtc acgcccttcg
     9901 ccttgacaag gtcctggatg ctcttgtcct cgtcgaggat gaacttgcga ttgaaggcgc
     9961 gggcatgggc ccaggcatcc atgacggcct tctggccagc cgcgtcgagc ttcgcccacg
    10021 aggccttcga catgatgagg gccacggcct ggtgcgtgtg ccgcacgtcg gcgacgttct
    10081 tcactacgtc atagaacttg gtgtggtaga aagtgttgac gccgccttcg gcaaaatcga
    10141 tcactttggt ctgcagcgag gtcgcaacct cggggaaggc gatcggcgtc gagatcatgc
    10201 cgagcgcctt gaacgtggcg accaggatct tgtcctcctg tacgcggatc ttcttgccct
    10261 tcacgtcggc gagcgagttg atcgggtagt ggccgtagat gccgcgatag ccggccgcga
    10321 tgaatccgac gagcttcagg cccttcttgt cgagcacgtc cgaatagtac ttggtgagcg
    10381 tatcggagcc atccagcgcc ttgaagacgt ggtcgtagtc gcggaaaatg tagggcagct
    10441 ggaacacact catctcgggg atgagggtcg agagcggcgc ggtcgtgatc ggaccgaaat
    10501 cgacggcacc gagctgaatc tgctgggcca cctcgcgctc gccgccgagt gcgccggagt
    10561 ggtggtggat cagcttcacc ttgccgccgg agagctcggc cagtttgtcg atgaaggcct
    10621 gcgtggtctg cgaggtcccg tgcttcggcg gattgacgtt gccgaccgtc atctcgcgca
    10681 cctggccgac ggcaccgcca gcgaatgtcg ccgcaagcgc cgcgaccgcg agcgcggtga
    10741 acgccccaac cgttctgccc gaccgttgtt gtcctgccat gtggtgctgc ctcccgagtt
    10801 gcaaagacgc gccgaaccct gatcccgatg catgcatcga gcctgcacag gccgtcttct
    10861 ttttttgact tgaggccatc ttgggtgagc ggactgacga gcgtcaagcg cgatccgccg
    10921 caaccacgtc ggattgcgcg gctagagctt gcgcaactcc aggaactcca ccgtcacacc
    10981 gtccgaattc tgcacatagg cgacacgccg gccctggttt gggcctgcgt tgatggcgat
    11041 gacctcgcct gtcggcgcga agccgtggtg agctgccatg cctagtgcct cctccatgct
    11101 atcgacatcg tagccgaggt ggttcgcccc catgtcgcag agccgtgcag cgacacgctt
    11161 tcggtcgtag ggcgcataat actggatgag ctcgagccgg tggccgtatc cctcgaggaa
    11221 ggcgatcgtc acgatgacgc cctcgatccc cgtcatccgg ctcatcagat cgggatcgcg
    11281 cggggcgagg gagaggagat cgtagccgag cccgtccatg aagaacggca gcagcggctt
    11341 gatctccttc accgtgagcg agatgtggtt gaggcctctg gccttgatcg gtgccatgtg
    11401 acgttgcctc gtgcccggct agcggatcaa ctaagtgcca aagccttcga caaggcaagg
    11461 ttggacgctg tgccgctaac aggcctcttc cgtgacaggc cccgcagcgg gcctggccct
    11521 tcaaaagtcc gaagcgatgc cgcctttctc ccaatcgccg tagcgcgtcg gatcgggccc
    11581 gggctgcccg ccgagttccg cgggccgccc ctcgcccgtc ccgataccgg cccgctttcg
    11641 ccgctcctca gcctcggcaa gcgcacgctt tgcggcctcc gagagcacgc gcggagggga
    11701 cgatgcgtcc acatcgttgt ttccgtctgc cggatcgtca ggcatgcacc gccctcctgg
    11761 attagtcgcc atcgccgctc tatatagcag ggccgacgga tgcaacggat cggacgttga
    11821 acgagcggcg cgcgccggcc ggatcggtcc tcgagcagga gcaggctgcc atgccactga
    11881 actacgcgaa gaccggcctg ctgctcgccg ttctcatggg tatcttcctg ctgatgggca
    11941 gcgtcatcgg cggccagacc gggctcgtcg tcgcctttgt gcttgcgctc ggcatgaacg
    12001 cgttctcgct ttggaagtcg gacaagatcg tcttgcgtat gcatggcgcg caggaggtcg
    12061 acgagcgctc ggcgcctgac tattacggcc tcgtgcgtga actcgcgcgg cgcgccgagt
    12121 tgccgatgcc gaaggtttac gtgatgcaca acccgcagcc caacgccttc gcgaccgggc
    12181 ggagccccgc gagttcggcc gtctgcgcct cgacgggcct cctcgagatg ctctctcgcg
    12241 aggagcttgc cggcgtcatg gcgcacgaac ttgcccacat caagaaccgc gatacgctga
    12301 ccatgacgat ggccgcgacg atcggcggcg cgatctcgat ggtcgcacag tggatgcagt
    12361 tctcggtgct gttcggccgc ggcggcgggc gtgaaggcgg caatcgcctc ctccagttcg
    12421 tcgccatgct gcttgccccg tttgccgcca tgatcgtgca gatggcaatc agccgctcgc
    12481 gcgagtacca ggctgacagg ctcggcgcgg ttatctgcgg aaacccgatc tggctcgcga
    12541 gcgccctcca caagatccac aatttcgcgc gccagatcag gaacccgagc gcggaggctg
    12601 tcccggcgac cgcgcatctc ttcatcatca atcccctctc ggggcggggg ttcgacaact
    12661 ggttctcgac gcacccttcg accgagaacc gtatcgtcga gttgcagaag ctcgcggcgg
    12721 agatgggcgt ctccggcggt gcgcccgcgg cagatgtcgc gacggccgag cgcccctcga
    12781 tcctgcgtcc gagccggcgc acgagcgaca atcgtggacc gtggggatga aggagcggca
    12841 aggccgaagc ggcggcagtc ggccgccgag cagcaaggcc gcacctcggg caccacatcg
    12901 cgggaagccg cacgatcgtc ccgcggccga gcgcaaagtc cgagaaaatg acgatccact
    12961 tgccgccggg cttgccgcac ggatcgcggc cgctgcgatt tgcgaaagtg tcgttgcacg
    13021 gggccagtcg ttcgacgatg cggcggacgc ctcgccgcgc ctcgccgtgc tcgagccccg
    13081 cgaccgcgcc ttcgcccacc tcattgcgct gacggtgctg cggcgcctct cctcactcga
    13141 tgcggcgatc aaccggcatc tgaagagcga gctgccgcgt gatgccgcgc acgcgcgcac
    13201 catcctccgc gttgccgccg cgcagatcat ctacctcgca acgcccccgc acgccgccat
    13261 cagcctcgcc gtcgaccagt gccgtctcga tcgtcgctcg agccgcttcc gcgggctcgc
    13321 caacgccgtg ctccggcgca ttgcggagcg tgtcgaggca ggactagagc cgtcggggcc
    13381 cgtacatcgc gacatcccgg tctggctcat ggcccgctgg cgagccgcct acggcgatgc
    13441 gacggcagag gctattgccg ccgcgtcgct cgtggaggcg cctctcgacc tcaccgtgag
    13501 atccgatccg cccaaatggg ccgagcggct ctccggcacc gtgctcgaca ccggctccgt
    13561 gcgtctagcc gaagcgcgtc gcgtggacac gctcgacggc ttcgcggagg gcgcgtggtg
    13621 ggtgcaggat gcggcagcag cactcccgcc gcggctgctg ggcgatgtga aggggcgccg
    13681 cgtcgccgat ctctgcgccg cacccggggg caagaccgtg cttctggcgg cggcaggcgc
    13741 gcgggtcacg gccgtcgagg cgtccgaaag ccgtgcgcgg ctgatccgcg aaaacctcgc
    13801 gcgcctgaag ctcgatgccg atgtcatcgt cgccgatgtg ctctcgctcg aaatgagcga
    13861 gaccttcgac gccgtcctcc tcgacgcccc gtgcacagcg acgggtacca tccgccgcca
    13921 tccggatatc ctgcatctga agcgtccgag cgacatcgcc aagctcgccg agttgcaagg
    13981 gcgcctgctc gacaaggcct ggcggctcct gaagcgcggc ggcgtactcg tctattgcac
    14041 ctgctcgctc gagtctgagg agggagtgca gcagatcgaa cgattgctgg cgagccgcgc
    14101 gccagttgcg atcgagccga tcgccgcggg ggaggccggc atcaaggcgc cttggctgac
    14161 ccctgccggc tacctccgca cgctgccctt tcatacgcca gagcgcgttc gttccgacga
    14221 aagtataaca ttgccgtttt cgggtaacgg catggatggt ttctttgctg cacggctccg
    14281 acggcttggc attccttcac cctgatcaag ggccacagac ccggacatat cgccgcaact
    14341 cgcttcaatc cccgaatact ggcgaatgcc acatccagca gtatttctgt tattctcccg
    14401 tgttgttgtc agcaaattat ccatggagta tcgaaggata tagaatgcga ggtcgtgctt
    14461 gattgatgag atgcagtatc gccggggcaa atatctggat gcctcagcgc ttgttactgc
    14521 tcagagtgga ggaatccatg gacgcccaac ctagacgcga gctgatcgag ctcacgagcg
    14581 atatcgtggc gtcctacgtc gccaagaacc ccgttggacc gactgatctg ccaggtctca
    14641 tccaaaacgt gcacgatgcc ctgaagcgcg cgcagaacgg ggcggccgag ccgatccgcg
    14701 acgagctacg ccccgcggtg cccgtcaaac gctccgtaac ccccgatcac atcgtctgcc
    14761 tcgaggacgg gcagaagttc aagtcgctga agcggcatct gcgcacgcgc tacaacatgt
    14821 cgccggaaga atatcgcgag aagtggggcc tcccgcacga ctatccgatg gtggcaccga
    14881 actatgcggc agcacgctct gagctcgcca agcgcatggg gctcggcacg cgcaagtgat
    14941 cgggcagtga ggggctcccg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnncgaggc agccacgccg atgctctgca gcgatcatcg gctcgagccg
    15061 ggccaccaat cgtcggcgac ctgcgatgct cgcgtctcgt atgtccgcga gttcaaaggc
    15121 accgagtcgg gccagattgc gctcgcggcg atagcggcag gcaaattgac cgaacgcgcg
    15181 cgatctgcag catgccaagg tgccgtctcc ggagccggaa cgacagcaag cctcgcacgg
    15241 gggaggtcag cggggatttt ccagtggcgc tcaatagaag cagcttagcc tgacgagccg
    15301 gccgcaggca cgccgtcact cgacgacctt cgggacgacg aagtagccgt cctccnnnnn
    15361 nnnnnnnnnn ntgtcacgtc ggcggccttc tcgccgtccg tcacgacgtc ggcgcgcgtg
    15421 aagagcttca tgtcgacgac gcgcgtcatc ggctcgatgc cgtccgtgtc gacctcgtcg
    15481 agctgcttca cccagtcgag aatgccgacg agctcgccgc gcaacttcgg cacgtccgca
    15541 tcggagatgc gaatgcgggc gaggcgcgcg atgcggcgca cggtcttctc gtcgacgtcc
    15601 atgggctgat cctgctgact ggtcacaccc taaatagcga ccgggcctgc ggcgcgcaac
    15661 actcgccgaa caggctcgtc gtcacgccgg gcgccgatag gcgagcgcgc catcgccaag
    15721 gtcccaatag accgacgtga cgatcccgag gcccgttcgc gtcaaaggca gcagcaaatg
    15781 ttcgttcggc gcatgctgcg agcaccccgc ataggagagc ggcagccagg cgcaaggcat
    15841 accgacgagg tactgcatga tccacgtcat gttcgagccc ccgccgttgg ggagcacgcg
    15901 gcagcgatcg ccggcagctc gcgagactgc atcccgcatc cagagcgcga acggatgatt
    15961 gtggtccgtg cggcttgccg tgaagagctc gctcgccccg tttggcggcc cgctcacctc
    16021 gaccatgccg aagccgtggt tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn tgcagcactt cgcgcacacc gtctgggatc agcggccgcc aggcctcgac
    16321 aaggatgcgt ccgcgccggt cggcgatgct cgccagcgca tgcgcaagga tcgtccccgg
    16381 attggcgagg agccctcccc agttccccga gtggtggcca ccatcgcgga gccggacctt
    16441 cagctcgaac gtcgcagcac cccggttgcc gagcgtgatg ttgggatggc tgatctccgt
    16501 acgcggcccg tcggaggcga agaagccgtc ggcagcgaaa tccgccttat tggccgcaac
    16561 gatctggtcg aggccgggac tgcccgtctc ctcgcccatc tcgatcaaga atttcgaatt
    16621 gaagccgagc cgcccgcgcg tcgcgatgac ggccccgatg gccgcgatgt gggcgagatg
    16681 ctggcccttg ttgtcgacaa cgccgcggcc gtaggcgcgc tcgccgtcga gggtgacgcg
    16741 ccaggggtcg cgcccctcgg cccagcgccc cgccatgccg agcacgacat cgccgtgacc
    16801 gtacccgatg atcgtcggca aggccgggtc ctcgatacgg gtcgccagca gcaccgggcc
    16861 ggcgcctccc ttcgggttat cgtagatgcg gcacgtgtag ccgaggcgcc cgaagcgcgg
    16921 cgtcatctcg cgatcgaggt agcggcggag gtccccctgc cgctccgggt tctggctctc
    16981 ggtcggtacc gcgacgagcg ccgcgagatc ggcgaggtag ccgccttggt gatcgtcgaa
    17041 ataggagaga gcctgcgaca aggcctcgtc ccgtgtcgat cgagacatcg ttgatctccg
    17101 ttcaagcggg gcgtctgccc cacgctcgtg tccagcggct ttggctatca cggtggcggc
    17161 gcagcgcaca gcgtctggaa agctgaccca atgtcccgct gaccgccaag aacggcagca
    17221 gtttcgatgc agcagccatg gtcgagtaaa tcgatgcctg cctcggatgc ttgtcgacta
    17281 tggcgcagcc cgaaggccgg ggccgagtgt caatcggacc ctgcctgagc aagtccgcca
    17341 tgtcgccatt gcgaaggatg ttgctctcaa atagcctgca atcgccgtcg accggttcgg
    17401 aacggggaga gaagcgcaat gacgtcgatg aagccaacca tgggcgctgt ttgcgctctt
    17461 gtcctcgccg gttcggctct aggctgcggt gactccaaca acaccaagcc accggtcgca
    17521 ggagattccc ctccacccgc cttgatcgga tcgtgggacc tggtccatca ggatcgcgtc
    17581 aatgacgtcg ccgcgcagaa gatcgtggtg accttcacac cggacggcaa gcatacgtgg
    17641 gggccggtca tgagcaaatg cacctatagg ttcaagaaac cagacaagct gatcatcgac
    17701 tgcacgaaga gcgtcgcagg tgccgagttc gagtacatcc tcacaagtgt cgacgccacg
    17761 gacctcgtgc tgaccaatcc cgcgaccaac cccgaccagg tgaacaagct gaaacgaaaa
    17821 tagacgcgtc ggatcgctag gctcgggatc tcctagcccc tcaccgcttc cgccgcgaca
    17881 gcaccacgac ggcttcgagg tgcggcgtaa agaggaactg gtcgattggc gttgcgcgct
    17941 cgatcgcgta gccgccagcg gccagcaccg cgagatcgcg gacgagcgtc gccggattgc
    18001 aggagacagc gatcacgcgt ggcactttcg agcgggcgag cgcctcggcc tggctcttgg
    18061 ccccgacgcg cggcggatcg agcacaacga catcgaactg gtcgagctcg cgcggcgaga
    18121 gcgggtcgcg catcaggtca cgcgctctca cctcgatcgg cttcaggccg ggattgcgcc
    18181 gcccggcctc ctcgatcgcg gcgagcgccg agcggtcgtt gtcgatcgcc atgacgcgac
    18241 cgagccgcgc gagcggcagc gcgaacgtgc cgacaccggc aaagagatcg gcgaaccgcc
    18301 caccgcggca ggcgccggcg agcacctcgc tgcgcatcgc ctgctcggcc atctccacgg
    18361 cctgcacgaa tgtgccgggc ggtggcgtca cgcggacacc gccgagatcg agctcgggcc
    18421 gcgctgtcat gagacagagc tcgcgcccga tggtgagcct cgcgatccga tgcgcgtccn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccgatcacg
    18541 acatcgaggc ccgttactgt cgaaagcacc gtgacgcgcc cggcggtcgt tccctcgagg
    18601 acatgcccca gaatttggcg gagccccggg atcgccgcca tgatctccgg ccgcatcacc
    18661 gcgcagtcgc gcaaatcgac gagcgcgtgc gaggcgaggc catggaagcc gatcgcgacc
    18721 gaaccatcac ggcgcacggc cgtcagcacg gcgcgccgcc gcgagcgcgc ctcgatcatg
    18781 acgagcggcg cgatcgcatc tacggggagc ccggccctgc gaaaggcagc ggcgagcagt
    18841 ccgcctttcc agtcggcata gatagcgtcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgc tccgggctcg gcgggcctgc
    18961 aagcgtcgcg ggctccctgg cgccaagccg ccaccgctcg ccaggcagcg cgcccgcgat
    19021 gatttcggcg ccggggcccg cgccgatccc gtctcccgag ctgccgagac gctcgacctc
    19081 gatcacgcgc gccgcactca tggcccgatt gcccccacca gaaactcgac attcttctcg
    19141 cgctcgattg gctcccagcg ctcaacgccg tgcaccgccc aaccgagcgc accgagcatc
    19201 gcggagacgc catccacggc atcctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnctgg ggtttcacga gagcgatcag
    19321 gcaggcttgc ggtcgagcga gcgcgagagc cgctggcagc gccttagcga gcgaaatgaa
    19381 actcacatcg gcaacaatca ggtcgatcgg gtcgggaatg aggtccgccg tgatctcgcg
    19441 gcagtccgtg ctttcgtgcg cgacgacgcg cgggtcggcc cggaggcgag cgtgaagctg
    19501 atctcgcccc acgtcgaccg catggatgcg cgccgcgccc cgcgacagca gcacatcggt
    19561 gaagccgccc gtagacgccc cgatatcaag cgcggaccgg ccagacgggt cgaagggcgc
    19621 gccgaaagca tcgagagcca ggatcaactt gtcgccgccg agagagacgt ggcgcccgcg
    19681 ccttcccgcc agcgtgatga gggctgccgc gctgaccgtg cggctcgcct tcagctcgac
    19741 agtcccattc accgtcacct cgccagcgag caccagtgcg cgcgcccgcg agcgactggg
    19801 cgcgagttgg cggcagacca aggcctcgtc gaggcgcatg cacgggactc catggagcgg
    19861 cggcgacccg gaggttatag ttgaaagcgg cggacgaaaa aacgtcatgc cgctcgccgt
    19921 ccagacgcct ctcgcctgcg tattgacaca tggtggccaa ccgccccaac ggacaaccgc
    19981 acctggagga cccaagatat ggaccgtcgt caaagcctga agctcatgct cggcgccgcc
    20041 ggcgccctcg ccgccccagc catcatcggc cgtgcgcccg cgctcgccca ggctccgaag
    20101 gccgcgcctg ccttcacgct gccgccgctc ggctacgcct acgaggcgct tgagccgcac
    20161 atcgatgcag ccacaatggg cttccaccac aagaaccacc acaatgcctt catcggcatc
    20221 ctcaacgcgc aggtcgagaa atggcccgac ctcgccaaga agccgattgc cgagataatt
    20281 tcgaacctgt cggcggtgcc cgagggcgtg cgcacacccg tccgcaatgc cggcggcggc
    20341 cactggaacc acaccttctt ctgggatctg atgacgcccg gcggcccgaa ggcgccgacc
    20401 accgaggttg ctgccgccat cacagcggcc ttcggctcgt tcgagaagat gacagaggcc
    20461 gtcaaccagg ccggcatgac gcgcttcggt tcgggctggg cctggctcgt ggtcggcaag
    20521 gacaagaagc tcgcagccgt caacactccc taccaggaca cgccgcacat ggacctcggt
    20581 gtgaagccga tcctcggcgt cgacgtctgg gagcacgcct actacctgaa gcaccagtat
    20641 cgccgcggcg actacctcaa ggcctggtgg aatacggtga actggaacaa ggtcgcggcc
    20701 aacttcaagg gcgcgatggc gtgacacagc acggcgccgg ccgcggaacg cgaggccggc
    20761 gccgcgcaat ctgatcagcg ccggccgccg aagatcgact tgatcacgga attcttgatc
    20821 tgattgaaga cgctgcgcgc aatcgagcgg gtcacgtcct gggcgacgtt ctctgccatc
    20881 gacctgccgc cgcgcggccc tttgccgaaa accgtattgc tccagctgtt ggcgttggcc
    20941 tccttctggc gcgtctcctc ttcggccgcg gctgatgccg ccccggcctt tttctggagc
    21001 atctcgtacg cgctctcgcg gtcgagtgcc tcctcgtact tcccgtagtg caggctatac
    21061 tggatgcagc cctgccgctc ctcgggcgtc accgggccga tgcggccgag cggcgggcgg
    21121 atcagcgtcc gctgcaccac cgacggaatg cccttggcct ccagnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gaaggtttcg
    21241 gccgccgcgc ggatcgcctt ctgctcgacg ggcgtgaagg cacgcagcgc atgctgcacg
    21301 cggttgccga gctgggccga cactgaattc ggcacatcgc gcgggttctg cgtcacatag
    21361 tagacgccga cgcccttcga gcggatgaga cgcgtcacgc gctccacctg ctcgagcagc
    21421 gccttcggcg cctcgttgaa caggagatgc gcctcgtcga agaagaagac gagcttcggc
    21481 ttctgcgggt cgccgatctc ggggagcttg tcgaaaaggc tcgagagcat ccagaggaga
    21541 aacgtcgcgt agaggcgcgg cgtcttcatc agccggtcgg cggagagaat gttgacgatg
    21601 ccgcggccgt cgggcgcgtg cctgatgaaa tcgttgatgt cgagcgctgg ctcgccgaag
    21661 aaattgttgg cgccctgctc ctcgaggacc aggaggcggc gctggatcgc accgaccgat
    21721 gtcgcggcgg cattgccgta cttcgactgc agctccttgc tgcgctggcc aacgtcgttg
    21781 accatcgcgc ggaggtcctt gaggtcgagc agcggcatct tttcgtccgc ggcgacgcgg
    21841 aacatgatat tgaggacgcc ttcctgcacc tcgttgagct cgaggagccg cgagagcagc
    21901 agcggcccca tctcctgcac ggtcgcgcgg acgggatggc catgctcgcc aaagatgtcc
    21961 cagaacacgg tcgggaaggc cgtgttgtca taggaggaaa gcccgacctc cttggcccgc
    22021 ttcaggagga aatccttcgg ctcgccgacg gccgcgatgc ccgagagatc gcccttgatg
    22081 tcggaggcaa ataccgggac gcccgccgcc gagaagccct cggccaacac ctgcagcgtc
    22141 accgtctttc cagttccggt tgcacccgtg acgaggccgt gccggttcgc aagcttcagc
    22201 tccaggtact cgggcttgac gctctggccg atgaagatga gcccgtcctt caacggacca
    22261 tcggaggctg attggcttgc catggcgcat tccctcgctc ggatgcggct tcccggatgc
    22321 agcgtcgcga ccgactcgcc ggccgcgaaa tgacgcgatc acatggcatg gcgcggcccc
    22381 atttgcaaac gccggttgca tgcgcaaagg cccaaacgtg gtagcagctc acggcgcgtc
    22441 cagagccggg ttgtgcagcg catgaagcgc acaccacgtc gctctagcgc cggcattcgt
    22501 tctttgctaa gaggcctcgc gtgatagtcg catggtaacc gggcctccag ggtccggcag
    22561 gacggtcgca ggggtattcc gcggtagctc aacggtagag caaccggctg ttaaccggtt
    22621 ggttgtaggt tcgaatccta cccgcggagc caaatctcgc aggccacgtt acatttctac
    22681 cagcgagaga agcctgcgcc cggggcggcc cccgcgcgcg taaggcgcgc tccgccagaa
    22741 gtgccccggt cgggtctggg cgaaagccca gtcgcgatgc gaaccgtggc cgcatacagc
    22801 cccaattgat ttgcggacga tccgccaacc tgccatccct gtcgcgaacc tgttcatccc
    22861 ccgatgaagc cggacgctcg cgatgtcaga tcacacacat caccacgact gctgtgccca
    22921 cgaccatcat cacggtcacg cacttcctgc agagccggca accaacgccc tcctgagcgc
    22981 gcggcggctc acgctgaagc gcgggcagcg cgtagtgctc gacagcgtcg atatcgacat
    23041 tcgcgcgggc gagatcgtca cgctgatcgg gccgaacggc gcgggcaaga cgacactcgt
    23101 ccgcgttctg ctcggcctcg agaccgccga cagcggcctc attcgccgca agcccgacct
    23161 cgtgatcggc tatgcgccgc agcgcttcga tctcgatccg gctatcccga tgacggttgc
    23221 acgcttcctc gcgacggctg cgacgcgatt ccgcgatgcg cgcgatgtgc ccgtcgcggc
    23281 gctcctggca gaggtcggcg cgagcgccct cgcggaacgc cagatcgccc gcctctcggg
    23341 tggcgagttg cagcgcgtga tccttgcgca tgcgctggcc cgcaagcccg agctcctggt
    23401 gctcgacgag ccggtgcgcg gcgtcgatta tctcggcgag gccgacctct acatgctgat
    23461 aggccgcatc cgctccgagc gcgggctcgg cgtgctgctc gtcagccacg atctccacgt
    23521 ggtgatggcc aacagcgacc gcgtcatctg cctcaaccgt cacgtctgct gccagggcat
    23581 cccgcatacg gtcgcgcagc atcccgagta tgtgcgcctc ttcggccccg aggccgcacg
    23641 ggcactggcg gtctaccacc accaccacga tcacgtgcac gatctcgcgg gcgccgcgat
    23701 ggccgccgcg aaggacgagc gctgacgttc aagcgggctt cgacgcggct atcggcgtgg
    23761 ccgccggtt
//
LOCUS       DAORNH010000027        23612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1649_length_23612_cov_2.527656, whole genome shotgun sequence.
ACCESSION   DAORNH010000027 DAORNH010000000
VERSION     DAORNH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23612
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_23612_cov_2.527656"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..966)
                     /gene="fmt"
                     /locus_tag="PK264_04750"
     CDS             complement(34..966)
                     /gene="fmt"
                     /locus_tag="PK264_04750"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRD75233.1"
                     /translation="MTLRIIFMGTPDFAVPTLSECLAAGHDVAAVYSQPPRPAGRGLA
                     ESRSPVHEFALASGLEVRTPRSLRSEEEQAALKALGTDVAVVVAYGLILPQAVLDAPR
                     LGCFNLHASLLPRWRGAAPIQRAIMAGDHETAACVMRMEAGLDTGPVCLVERVEITPA
                     MTAGDLHDLLARQGAGLMVRALAALERGSLTETPQPAEGVTYAAKIEKAEARIDLGRP
                     AREVLNHIHGLSPFPGAWLEAGPAGARERLKVIRAEITAGRGPAGELLDDRLTVACGS
                     GAVRLVEVQRAGKRPMSAEELLRGFQLRRGDRLG"
     gene            1192..1980
                     /locus_tag="PK264_04755"
     CDS             1192..1980
                     /locus_tag="PK264_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75234.1"
                     /translation="MTFTKLLLNSAAGALLASTIANGAAAQTFDVKPVEVTKGALDFG
                     IDNTFHRGVPHDRGSDINRSAHDIGLDYGLLAWWRISGVIKLENVEEDELRASRVAFE
                     NLFVLRPVDDRKPFDIGLGWFTAVEASIHPDTTNALVFGPIVAIKSDKIVFTANPFFE
                     QTFGRNSVDGIAFTYGWNAKYEIREGFALGIEGYGLIENIGNPPPWSEQEHRIGPAIF
                     TEFKLGNGLTIAPDIGLLFGMTHATPDVALKLNIGVPLIKPAGG"
     gene            complement(1999..4475)
                     /locus_tag="PK264_04760"
                     /pseudo
     CDS             complement(1999..4475)
                     /locus_tag="PK264_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(4649..5539)
                     /gene="rpoH"
                     /locus_tag="PK264_04765"
     CDS             complement(4649..5539)
                     /gene="rpoH"
                     /locus_tag="PK264_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HRD75235.1"
                     /translation="MSTKALPSIAGGAGGLTRYLEEIRRFPMLEPQQEYMLAKRWVEH
                     QDPAAAQTLITSHLRLVARIAMGYRGYGLPIGEVIQEGNVGLMQAVKRFEPDKGFRLA
                     TYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRRAKSELQALEDGDLKPEHVK
                     AIATKLQVPEEDVVNMNRRLAGDMSSNAPRRADTPAGGEWQDWLEDDAPSQEEVLADR
                     EEGDLRRGFLAKALTGLNDREKRIFEARRLRDEPATLEDLSAEFGVSRERVRQIEVRA
                     FEKVQAAVQAASREAVKPKA"
     gene            complement(6058..7218)
                     /gene="dapE"
                     /locus_tag="PK264_04770"
     CDS             complement(6058..7218)
                     /gene="dapE"
                     /locus_tag="PK264_04770"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HRD75236.1"
                     /translation="MDATDPVALAAALIRCQSVTPDEGGALSLIADVLGPLGFTSHRL
                     TMTEPGTPDVENLYTRLGTGKPNLCFAGHTDVVPPGDPKAWALPPFSGEIRDGLLHGR
                     GAVDMKGAIACFMAATARHLKSRGGAIPGSISFLITGDEEGPSINGTMKVLDWMARRG
                     ETMEAAIVGEPSNPKQLGEEIKIGRRGSLNGELVVAGKQGHVAYPQLADNPVPKLIGM
                     LEHIDGLILDDGTPDFEPSNLEITVISVPNTATNVIPSEARAKFNIRYNTRWTRPRLE
                     ALLRETCAAAAVEVEAQYKLGFSGTGDVFLTKPGPLVDHMRAAVMQVTGRTPALTTNG
                     GTSDARFIQAYCPVIEFGLTNALAHHVDERVAVADLEALTRIYEAFLGRYFA"
     gene            complement(7282..>7973)
                     /gene="prmC"
                     /locus_tag="PK264_04775"
     CDS             complement(7282..>7973)
                     /gene="prmC"
                     /locus_tag="PK264_04775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRD75237.1"
                     /translation="LTRRLAREPVWRILGQRAFYGLELQIGPAVLDPRPDTETLVDVV
                     LEATADAGAAAPLRILDLGTGSGAILLALLHERPSARGVGVDRSAAALQIARRNGEQL
                     GLNDRVAWIESDWFAGVSGRFDVIVSNPPYIPTGEIAGLDPEVRNHDPRAALDGGGDG
                     LDAYRAIVAGVAQHLAPGGLVAFEIGDIQGPAVSALMFSAGLELVGGVRRDLSERPRV
                     VVARAPVGKSP"
     assembly_gap    7974..7983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7984..8190)
                     /locus_tag="PK264_04780"
     CDS             complement(<7984..8190)
                     /locus_tag="PK264_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75238.1"
                     /translation="MSRGPRPISGGMSRAEARQLIARELAAAGIDGAEGDADHLVAGI
                     LGLAWIDLVARGEEPVGALADRLVA"
     gene            complement(8187..9269)
                     /gene="prfA"
                     /locus_tag="PK264_04785"
     CDS             complement(8187..9269)
                     /gene="prfA"
                     /locus_tag="PK264_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRD75239.1"
                     /translation="MTAIPPEKLDKLVGRWDAVQAELNSGVDPQTFARLSKEFAELDP
                     VVASIRELQRLEGELASAREMAAGADGELASMAREEIGAIEPRLAELGETLKVQLLPK
                     DAADERSIILEVRAGTGGDEAALFAGDLFRMYSRYADLRGWKTEVISMSENDLGGLKE
                     VVASITGSGVFARLKFESGVHRVQRVPQTEASGRIHTSAATVAVLPEAEEIDVEIKAE
                     DLRIDTMRSSGPGGQNVNKTESAVRITHLPTGIVVTSSEKSQHQNRRTAMGVLRSRLF
                     EFTRQKAADERAAQRKGQVGSGDRSQRIRTYNFPQGRVTDHRINLTLHKLPAVLDGTA
                     LDELVEALITDHQATLLAEMNEQAGA"
     gene            complement(9283..>9711)
                     /gene="leuD"
                     /locus_tag="PK264_04790"
     CDS             complement(9283..>9711)
                     /gene="leuD"
                     /locus_tag="PK264_04790"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRD75240.1"
                     /translation="PWRKARMIVSRENFACGSSREHAAWALNDFGIRAVIAISFGDIF
                     FNNCFQNGMLPVRLKPEEVDQVMAAAEAGDPVTIDLETQTVSIPQSEKNRHGATFKFE
                     IDPFRKRCLLNGLDDIGITLEHEGRIKDFETAHRTRHAWA"
     assembly_gap    9712..9731
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9732..9913)
                     /locus_tag="PK264_04795"
     CDS             complement(<9732..9913)
                     /locus_tag="PK264_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRD75241.1"
                     /translation="MDKFVRLSGIAAPMPINNIDTDMIIPKQYLKTVERKGLGVGLFR
                     EHRFDEAGKEKPDFVL"
     assembly_gap    10111..10216
                     /estimated_length=106
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10217..>11172
                     /locus_tag="PK264_04800"
     CDS             <10217..>11172
                     /locus_tag="PK264_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRD75242.1"
                     /translation="PFASLRANGRKVREPKRTLAVVDHNLTTGDRKKGFPDPEGRLQM
                     ELLDKNVKEFGIEYYDAFDKRQGIVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGAIA
                     HGIGTSEVEHVLATQTLMQKKAKNMRITVKGTLPKGATAKDVILGIIGEIGTAGATGH
                     AVEYAGPVIEALSMEGRMTVCNMSIEAGARVGLIAPDEKTYAYLKGRPKAPSGKAWDE
                     AVAFWRTLKSDPDAKFDREISIDVGKLAPLVTWGTSPEQVVAISGVVPDPAKIDDPAR
                     RENVERSLAYMGLTPGTRMTDVTIDRVFIGSCTNGRIEDLRE"
     assembly_gap    11173..11191
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11192..11501
                     /locus_tag="PK264_04805"
     CDS             <11192..11501
                     /locus_tag="PK264_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRD75243.1"
                     /translation="KKVSPNVYAMIVPGSGVVKAEAEAEGLDKVLKAAGFDWREPGCS
                     MCLAMNDDKLKPKERCASTSNRNFQGRQGPGGRTHLVSPAMAAAAAIAGRFVDVRQLG
                     "
     gene            11524..>12069
                     /locus_tag="PK264_04810"
     CDS             11524..>12069
                     /locus_tag="PK264_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HRD75244.1"
                     /translation="MPLTAAYRPDPRHFTLGEAFFDEVGPASFPKTILRVRNDRWARR
                     IGIEALSDDEWVRHLARFEALPANIRKPLALRYHGHQFRVYNPDLGDGRGFLFAQMRD
                     SVDDRLLDLGTKGSGQTPWSRRGDGRLTLKGGIREVLATEMLEALGVYTSKSLSLIET
                     GEALTRNDEPSPTRSSVLVRLS"
     assembly_gap    12070..12202
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12203..12992
                     /locus_tag="PK264_04815"
     CDS             <12203..12992
                     /locus_tag="PK264_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HRD75245.1"
                     /translation="RMSVAAVAEMGAEWFQAGFVHGVLNTDNTVITGESFDYGPWRFL
                     PTLDPAFTAAYFDETGLYAYGRQAGALFWNLQRLAECLLPLSSEAALVAALETFGSAY
                     QTAVARFTLARLGLADQDGRGLDEIVRPFYAALAAANIPFERAFFDLVGGARKDRIAA
                     SPYHIVYDAEPFASLTALMRDWPKAAGLEAALAHSYFADQAPRTMLIDEVEAIWAPIA
                     ERDDWQHLHKAIAAIRQMGAAHAPLIPSRASQPAHRRFVVAAQP"
     gene            complement(13081..13830)
                     /locus_tag="PK264_04820"
     CDS             complement(13081..13830)
                     /locus_tag="PK264_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD75246.1"
                     /translation="MKWFKSVGAAFAIAAVASGSGTAPAVAHDHNWGGVYFGINAGWA
                     GKDFDWAFNPPILGAAHQAYSLSDDGGLIGGHIGIQHQWNNLVVGVEAAYSGSSVFGN
                     DWAREPMFGNNPAFDSLVRMNSIFTVGPRIGFAPNNQWLFFIGGGFASANVQSTAINL
                     ATGAHTFSANERHNGWYLGGGIEYAMTHNVILGVEYQHIDLGTEIHCPGPCLGAAGGF
                     VDRDIDASVDVVRARLSIKLGRPVEHPKPMK"
     assembly_gap    14053..14075
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14242..15039
                     /locus_tag="PK264_04825"
     CDS             14242..15039
                     /locus_tag="PK264_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HRD75247.1"
                     /translation="MTPPPRAATGADARRMFRKGEFTGATHGIAPGYVQGNLAILHKD
                     LAPDFVRFCQLNPKPCPLIGMSPKPGDPTIPELGADLDIRTDLPKYRLWRNGELTDEP
                     ADIRQAWNNDLVAFVLGCSFSFEEALVEQGIELRHMACGCNVSMWRTSIPCTPVGPFH
                     GPMVVSMRPLKPVDAIRAVQVTSRFPSVHGAPVHIGKPELIGIRDINEPDYGDAVEVR
                     DDELPVFWACGVTPQAVIAAMKPEFSITHAPGSMLITDLLNSRLAVI"
     gene            15047..15799
                     /locus_tag="PK264_04830"
     CDS             15047..15799
                     /locus_tag="PK264_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75248.1"
                     /translation="MDLAIKGKAALVTGASTGIGRGIALALGREGVRLAITARRGELL
                     SEVAREIEAAGAPPPVTIVQDVMAEDAPLRIAKAAVAGLGGVDILVNNAGGSRQFGKD
                     ATEAQWTEAMTLNFHRPRQLAHQLLDQMVARKWGRIVNITGKSEPEGVNGAFSAKAGI
                     HAWAKGLSREVGKLGITVNCIPPGRIMSEQIRRNYTPEYRKWQAEHEIPVGRYGEPED
                     LAALVCFLASPLAGYITGTVIPVDGGLRRYQF"
     gene            15965..16711
                     /locus_tag="PK264_04835"
     CDS             15965..16711
                     /locus_tag="PK264_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HRD75249.1"
                     /translation="MRGSKRLAQGAAFALALMATAAVAQDTKKGAPPGSPVGGTWSAP
                     KVVPDAPTGGRTFEPKEIEAIKRVSEYFNALTTLKGRFVQSVADKEKRPMRGRFYLQR
                     PGRMRFEYGAPSKQLVVADGKMLAIQDLDLETDDQIALDQTAFRMLLRPDVDLIRDAR
                     ILDLQEADDVIILTLVDKNPDAAGKIKLFLVKKPGLELKEWVTVDAQGIETRVEVGEL
                     AFDPQLVKGETIEPKLFVIEPISLKKKTGQ"
     gene            16884..17900
                     /locus_tag="PK264_04840"
     CDS             16884..17900
                     /locus_tag="PK264_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRD75250.1"
                     /translation="MDRRGGAISTRIVNRVLAALAIAGLMIAGAPIVAQPSRPDLGAL
                     KALFRRPDRIPHPVDAPLTPERIALGRTLFFDPRLSSTGVASCASCHDPRRAFTDGGG
                     RGVTGRSTKNAAPMLWNLAWARALFWDGRAPTLEAQLRGPIESPDELGTSLQDIARRL
                     NADTALRTSFARAFPEKTEIDSALVVAALAAFERSLVSPPTRFDQWIAGDSVALTRAE
                     IDGFTLFTGKAGCSVCHGGWRFTDDKRHDIGRPITTEREDRNPAVKTPTLRELTWTAP
                     YMHDGSLPTLESVVDHYADRIIDRATLAPELRRRQPLSQTERSALVTFLMSLSGASPP
                     PLPD"
     gene            18020..>18466
                     /locus_tag="PK264_04845"
     CDS             18020..>18466
                     /locus_tag="PK264_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD75251.1"
                     /translation="MHRRTLLKGLGLAAGTIAAPAVLRAQAPITLNGAVQFDDNHAFT
                     KALTRFGELVNKYYGKPVNFTLHKNSSLGLEKQYFEYMAQGKAVDFAIVSPAHMSTFA
                     KAAPFIDAPFVFRDIAHMNAVVGKDLLKPIADEVAQKAEVMLIDQVT"
     assembly_gap    18467..18476
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18477..18997
                     /locus_tag="PK264_04850"
     CDS             <18477..18997
                     /locus_tag="PK264_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD75252.1"
                     /translation="KVITNLAELKGLKVRVQGAPIWSRTFTAAGMSPTVIAYNEVYNA
                     IQNGVIQAGENEAAGIEAMKFFEVAPNIILTQHAVSIRPICFSVKIFKTLPKELQDAI
                     VKAGKEAGDFGRGLESSEDGAKLEALEKAGRLKRIPFADRAAMAKLVEPVMDAYAKEI
                     GASEIAAKIKAA"
     assembly_gap    19300..19337
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19625..20512
                     /locus_tag="PK264_04855"
     CDS             19625..20512
                     /locus_tag="PK264_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRD75253.1"
                     /translation="MSNLQIFVPAAPGGGWDATGRAIELSMKTDGIVKDFRFEHAPGA
                     GGAVGLPKFLQTKKGQGDALMVGGMVMVGSLIANKSPVSMKDLTPVARLTGEANVLVV
                     PAASPIKDIKGWVDAFKADPGKMSVAGGSAGGTDHILMGMIAKALGKTAKNVSYVAYA
                     GGGPAVAAILGNQVACGTSGVGEFAEHVKSGKMRAIAVSGASRVAGIDAPTLVEAGIN
                     VELVNWRGVFGAPGLTSDQTKTLVDTVDRMVKGPTWQAELKKKDWANVYLGGAEFGKY
                     LDAEIGRITSILKDLGLAT"
     gene            20584..21069
                     /locus_tag="PK264_04860"
     CDS             20584..21069
                     /locus_tag="PK264_04860"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRD75254.1"
                     /translation="MHKNVAWQETVLGLFVIALAAVVGWQTMVIPENAIYAKIGPRFF
                     PWIATALLVVMGVLLTVQGLQGGWEHELDDDPDLLAVGWLAGGLVFNVLFIGGAFGLP
                     RLGFILCSTVLFTCVARAFGSRQPVRDAAIGFLLALVCYVGFDRVLGYKIGSGPIEGL
                     I"
     gene            21071..>21371
                     /locus_tag="PK264_04865"
     CDS             21071..>21371
                     /locus_tag="PK264_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD75255.1"
                     /translation="METLGLLLQGFQTALTPVNLVWCFVGVFVGTAIGVLPGLGPALT
                     IALLLPLTYKVDATAAFILFAGIYYGAMYGGSTTSILLNTPGESATIVTAMEGNKM"
     assembly_gap    21372..21381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21382..22561
                     /locus_tag="PK264_04870"
     CDS             <21382..22561
                     /locus_tag="PK264_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD75256.1"
                     /translation="AGAALSTAAIVSFVAGTIGTIGITFLAGPVVEFALWFGPAEYFS
                     LMILAFVTVSAVLGNSAVRGLTCLALGIFFGMIGIDLQTGQPRFWFGVTELSDGINVI
                     VVAVGLFAVGETIYLASKRRSGTSDEVLEVKDSIWMSRQDWSRSWKPWLRGTAIGFPI
                     GAMPAGGSEVPTFLSYYTEKKLSKHPEEFGHGAIEGVAGPEAANNASAAGVLVPMLAL
                     GLPTSATAAMMLSAFQSYGITPGPQLLQQQANLVWALIASLYVGNVMLVVLNLPLAAL
                     WVKLLKIPEPLLYGGILVFATIGTYGISQSTVDLVLLYIVGFLGFLMRKFDFPTAPVI
                     IGMILGPLAEQNFRQALTISQGDMTVFFTRRLSLALLLLALLALVGPRLWQLVRRRPS
                     "
     gene            complement(22595..>23262)
                     /locus_tag="PK264_04875"
     CDS             complement(22595..>23262)
                     /locus_tag="PK264_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HRD75257.1"
                     /translation="AIMFAVLPAPVAEVLSPQVVAVMGQSQYGLLTIGAAVALLFATS
                     AIESLRAALNTAYHVQETRSYLWCLLQSSLIVFASALGALALAAGVIIGPVIVWTWKS
                     PTLDWLIEQGYFNVGFRYTVVTLILLGQLFVYHLWLAAGRRKVREVWPGVVLSIALWL
                     LVAAFYSRYLILNDYSRFYAGLTQLMSAFIFFQVTSMIIILGAEFNRCLAEASDELPT
                     SPG"
     assembly_gap    23263..23389
                     /estimated_length=127
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3982 a         7882 c         7346 g         3906 t
ORIGIN      
        1 ggcagctcga agtcgaagag atcggtgcgc atctcagccg agccggtcgc cgcgccgcag
       61 ctgaaacccg cgcagcaact cctccgccga catcggccgc ttgccggcgc gctgcacctc
      121 gaccagccgg actgcgcccg atccgcaggc gaccgtcagg cgatcatcga gcagctcgcc
      181 tgcagggccg cggccggccg tgatctcggc acggatcacc ttcagccgct cgcgtgcgcc
      241 agccggcccc gcttcgagcc aggcgccggg aaacggcgac aggccgtgaa tgtgattgag
      301 cacctcgcgc gccgggcggc caagatcaat gcgcgcctcg gccttttcga tctttgcggc
      361 gtaggtgaca ccctcggccg gttgcggcgt ctcggtgagg ctgccgcgct cgagcgccgc
      421 cagcgcccga accatcaagc ctgcaccttg gcgagcgagc agatcgtgaa gatcgcccgc
      481 cgtcatcgcg ggcgtgatct cgacgcgctc gacgaggcag accggacctg tatcgaggcc
      541 cgcttccatg cgcatcacgc acgctgccgt ctcgtgatcg ccagccatga tcgcgcgctg
      601 gattggcgcc gctccccgcc agcgcggcag gagcgacgcg tgcaggttga agcagccgag
      661 ccgcggcgca tcgagcacgg cttgcggcag aataaggcca taggcgacaa ccaccgcaac
      721 atcggtgccg agcgccttca gcgccgcctg ctcttcctcg ctcctcaaac tccgcggcgt
      781 gcgcacctca aggcccgatg ccaaggcgaa ctcatgcacg ggcgagcgcg actcggccaa
      841 accgcgtcct gcaggcctcg gcggctgcga atacacagcg gcgacgtcgt ggcctgcggc
      901 caagcactcc gagagcgtcg gcacggcgaa gtcgggcgta cccatgaaga tgatgcgaag
      961 ggtcatggcg ctatttcggc gccctcctgg gcggggtcaa gagttagagc catcgcgaag
     1021 cctggaccag cggcggctcg gttgatccga atgcgtatcg cttcggcaac gccagaacgg
     1081 caatcgtcga tcatggggag catttcaaga ctgatcacac gttgcccgat gtagaacatg
     1141 ataactgaag cccgtggtgc gcgtatgacc tgggacaggg aacggtctcg catgacgttc
     1201 acgaagctcc tgctgaattc ggccgccggc gcgctcttgg cctccaccat cgccaacgga
     1261 gcagccgcac agaccttcga cgtgaagccc gtcgaggtca cgaaaggtgc gctcgatttc
     1321 ggtatcgaca acacgttcca tcgcggcgtc ccccacgatc gcggctcgga catcaatcgc
     1381 agcgcgcacg atatcgggct cgactacgga ctgctcgcgt ggtggcgcat ctccggcgtc
     1441 ataaagcttg aaaatgtcga ggaagacgag ctcagggcct cgcgcgtcgc tttcgagaac
     1501 ctgttcgtgc tccgcccggt cgacgacagg aagcccttcg acatcggcct cggctggttc
     1561 accgcggtcg aggcctcgat ccatcccgac acgacgaacg cgctggtgtt cggcccgatc
     1621 gtcgcgatca agtcggacaa gatcgttttc actgccaacc cgttcttcga gcagactttc
     1681 ggccgcaaca gcgttgacgg cattgccttc acatacggct ggaacgcgaa gtacgagatc
     1741 cgcgagggct tcgcgctcgg catcgagggc tacggcctca tcgagaacat tggcaatccg
     1801 ccgccctgga gcgagcagga gcaccgcatc ggccccgcga tcttcaccga gttcaaactc
     1861 ggcaacggcc tcacgatcgc tcccgatatc gggctgctat tcgggatgac acatgccact
     1921 cccgacgtcg cgctgaagct caacatcggc gtgccgctga tcaagcctgc gggcggctga
     1981 tcgatcatcg ggcacacctc agccccgcat cctcgctcca agcggatcat acatcggcac
     2041 gaggctcgcg actgccgggc agcgctcgcc cgcgacctcg atctcgaagc ggcgcgcggc
     2101 gatctggtgg ccagtctcgc cgcgctcgat cggcacatag ccaagcccga tcgcggcgcc
     2161 gagatgatgg ccgtaggcgc ccgacgtcag atagccgacg accttgcccg actcgatgat
     2221 cggctcggta tgataaagca gcggttcggg gctagcgagc ttcagctgaa cgagccgccg
     2281 ggtgaggccc gcctcgcgtt tcgctgtgac ggcatctcgc ccgatgaagg cgccgaatcg
     2341 acccgccggc ttctcagtct tgactgcaaa cccgagaccc gcctcgagca cgtggtcgtc
     2401 ctcgccgatg tcatgaccga aatgccggaa ggccttctcg acgcgcaggc tctccattgc
     2461 cagcgtgccg acgagcgtaa ggccgaaggc ctcgcccgca gccgtgaccg tcgagaacac
     2521 atgccgcacg aactcggtcg gcatgtagag ttcgtagccg agctcgccga cgtagctcac
     2581 gcgatgcgcc ctgaccggcg ccatgccgac gtcgatcatg cgggtggttg cgaaggggaa
     2641 catggtgttt gagagatcgg ccgtcgtcag cccctgcagc atctcgcgcg agcgcggccc
     2701 catcacggca aggcaggctt ccccgctcgt cacatccagc gagacgcagc gcgcgtcggc
     2761 cggcgtatgg cggcgcagcc attcgagatc acggagcacg gtcgccgtcg acgtgatcac
     2821 caggaacgag gtctccgaaa gccgagtcac ggtgaggtcg gcctcgatgc cgccgcgccg
     2881 attgagccag gtcgtataga cgatgcggcc gggcggcacg gcgacgtcgt tcgccgagat
     2941 gcgctggagg accgcttcag cgtctgcgcc ctcgactcgg atcttgccga acgtgctcat
     3001 gtcgagcagc ccgacgccgg tccgcaccgc cctatgctcg gcagcgacat tggcaaacca
     3061 cggctggcgc ctccagccat gagaatagtc tggcgccttc ccagcagtgc gctcggcttt
     3121 cgagaggaac cagtgggctc gctcccagcc cgccgtctcg ccaaaggcgc cgccatgcgc
     3181 cttcaattgg tcgtagagcg gcgtgtggcg cacgccgcga gcgctcgcga actggcggcc
     3241 tggataatga tctgcgtaga gcagtccgag cgtctccgtc acgcgggtgg tgatgaagct
     3301 ccgcgtcgcc tgatacggga acgtgcgccg gacatcgaca gcccagaggt cgaacggcgg
     3361 ctcgccggca acgatccact ccgcgagtgc cttgccggcc ccgccgactg gatgccaatc
     3421 gagttgaagc cgcaggcaac gtagagcccg gcgacaccgc gcgccgcgcc gagcagatag
     3481 cggttgtcgt gcgtgaaact ctcaggcccg ttgaagaacg tgtggatgcc gagcttctcg
     3541 aggatcggca gccgtttcga ggccgtctcc aggatcggcg cgaaatgctc catgtcctca
     3601 ggcagctggt cgaagcagaa gctctcggga atgccgccga cggcccaggg cttcgccttc
     3661 ggctcgaagc agccgagcag gatcttgccc gcgtcctcct tgtagtaggc gcactcgtcg
     3721 ggcacgcgca gcaccggcag gttgcgcggg atatctgaac ttgcctcggt gacaatgtag
     3781 aagtgctcgc aggcctgcag cgggagcgcc aagcccgcca tgcggccgac ctcggcgccc
     3841 cacatgccgg ccgcgttgac gatcacctcg gctgagatcg gtccgtgctc ggtcgtgacc
     3901 cctttcacgc ggccctcgac cagcgtgatg cccgtcacct tggtgttctc gaagattttc
     3961 acacccttct gccgcgcgcc cttggccatg gcgagcgcga cgttggccgg gtccgcctga
     4021 ccgtcggtcg gcaggaagaa cgcgcctttt gcatcgccga cgttgagaag tggatggcgg
     4081 cggcgcgcct cagcgagcga gatcggctcg acaggcacgc catatgatcg cgccatggag
     4141 gccgcgcgct tcagctcctc gaggcggtcc tcggtcaagg cgaccgacat agagccgact
     4201 cgcttgaagc cggtggacac gcccgtctct tcctcgatgc gcgaatagag ctcctgtgta
     4261 tatcgcgcga gcttggtcat gttggaagtg gcccgcagct ggccgatgag gcccgccgcg
     4321 tgccacgtcg tcccggaggt caactgcttc ctttcgagca gcacgacgtc ggtccagccc
     4381 aagtgcgcga ggtggtacgc caccgagcag ccgacgacgc cgccaccaat gatgacaaca
     4441 cgaaccgtgt caggcaggcg cgggggcgtg gacatgtgcg ggctcggctg gtttgggtga
     4501 gaaaccctac aggcgcatgc ccgagcgcgc tacccaccgc ggagcaggtg gggagcatca
     4561 cggagccagt tgccgcctgc cgctgaatac gaacgtggcg ccacccagcc cgagtggcgc
     4621 cacgaaattg gctggcagtc actttccgtt acgccttcgg cttcacggcc tcgcggctcg
     4681 cggcctggac ggcggcctgc accttttcga aggcacgcac ctcgatctgg cggacgcgct
     4741 cgcggctgac cccgaactcg gccgacaggt cctcgagcgt tgccggctcg tcgcggagcc
     4801 ggcgcgcctc gaagatgcgc ttctcgcggt cgttgaggcc ggtcagcgcc ttggcgagga
     4861 agccgcgtcg caagtcgcct tcttcgcggt cggcgagcac ctcctcctgg ctcggcgcgt
     4921 cgtcctcgag ccaatcctgc cactcgccgc ccgccggggt atcggcacgg cgcggcgcgt
     4981 tcgacgacat gtcgccggcg agacgccggt tcatgttgac tacgtcctcc tcgggcacct
     5041 gcagcttggt cgcgatggcc ttgacgtgct caggcttgag atcgccgtcc tccagggcct
     5101 gcagctcgct cttggcgcgg cgcaagttga agaacagctt cttctgggcc gcggtcgtgc
     5161 ccatcttcac gaggctccac gagcgcagga tgtattcctg gatcgacgca cggatccacc
     5221 acatcgcgta ggtcgcgaga cggaagccct tgtccggctc gaagcgcttc acggcctgca
     5281 tgaggccgac gttgccttcc tgaatgactt cgccgatcgg cagtccgtag ccgcgatagc
     5341 ccatggcgat gcgcgcaacg agacgaaggt gcgacgtgat cagtgtttgc gccgccgccg
     5401 ggtcctggtg ctcgacccag cgcttcgcca acatgtactc ctgctgcggc tcgagcatgg
     5461 gaaagcgccg gatctcctcg agataccggg tcagcccgcc agctccgcct gcgatgcttg
     5521 gaagcgcctt ggtgctcatc ttctttccgg cgctggctcg gcttttcagc cgctcctcgc
     5581 gccgctccct ctgggatcgt gaccgtctac gctcgtcgtc gaaagtctcg tgctctacta
     5641 gggtcttagc ctgctcccct gctctcgttc aaaccacgtt gctgtgtgct ccccttcacc
     5701 aacgatcacg tcggggcgag gagatggtga taagcttttc tacgaacaag tcctcaccgc
     5761 ggatttttca catgccctca aaacgatgaa acgaggtgca tccgtcgatc aaaggtgaac
     5821 cgctcatgta tgtttgacgg ctcatctgct ccgatccttg ggttgagcgt gccgtgtact
     5881 cgatgccaga agcctcaaga gtggacgcca aaccaccggg cggccgacgc tgctcgtgct
     5941 caagtgctga acatgctcca cggagagccg ctttttgcga gttcgtctcg ggcgtcggct
     6001 gttgcaggcg cgcagccaca gtgtgaccgt aaggcacgcc tgagcatcag atagaggtca
     6061 cgcgaagtag cgaccgagga acgcctcata gatccgtgtc agcgcttcga ggtcggcgac
     6121 ggccacccgc tcgtcgacat gatgcgcaag cgcgttggta agcccgaact cgatcacggg
     6181 gcaataggcc tgtatgaatc gcgcgtccga ggtgccgccg ttggtcgtga gcgccggcgt
     6241 gcgccctgtg acctgcatga cggccgccct catatgatcg accagcggac ccggcttcgt
     6301 gaggaacaca tcgcctgtcc cggagaagcc gagcttgtat tgggcttcca cctcgaccgc
     6361 tgcggcggcg cacgtctcgc ggagcagcgc ctcgagccgc gggcgcgtcc agcgcgtgtt
     6421 gtaacggatg ttgaacttgg cgcgcgcctc ggacgggatc acgttcgtcg ccgtgttggg
     6481 aaccgagatc accgtgattt ccaggttcga gggctcgaaa tcgggcgtgc cgtcgtcgag
     6541 gatcagcccg tcgatgtgct cgagcatgcc gatgagcttc ggcacaggat tgtcggcgag
     6601 ttgcggatag gcgacgtgcc cttgcttacc cgcgacgacg agttcgccgt tcagcgaccc
     6661 gcgccggccg atcttgatct cctcgccgag ttgcttcgga ttggagggtt cgccgacgat
     6721 cgcagcctcc atcgtctcgc cgcgccgagc catccagtcg agcaccttca tggtgccgtt
     6781 gatcgacggg ccttcctcgt cgcccgtgat caggaatgag atcgagcccg ggattgcacc
     6841 gccccgcgac ttcagatgcc gcgcggtcgc cgccatgaag caggcgatcg cgcccttcat
     6901 gtccaccgca ccacggccgt gcagcagccc atcgcggatc tcgcctgaga acggcggcag
     6961 cgcccacgcc ttgggatcgc ctgggggaac gacgtcggta tgcccggcaa agcagaggtt
     7021 gggcttcccc gtgccgagcc gcgtgtagag gttctcgacg tcgggcgtgc caggctccgt
     7081 catcgtgagg cgatgactgg taaagccgag cgggccgagg acatcggcga tcagtgaaag
     7141 cgcgccgccc tcgtcgggtg tcacgctctg gcagcggatg agcgcggctg caagggcaac
     7201 ggggtcggtc gcatcgatgg gcatggggtt cgccgcggct tttgaggtcc agaagtcaac
     7261 tatcagtgga acagacgagg gtcacgggga tttcccaacc ggggcccgcg ccaccaccac
     7321 gcgcggtcgc tcggagaggt cgcgcctgac gccgccgaca agctcgagcc cggcactgaa
     7381 catcagcgcc gagacggccg gcccctggat atcgccgatc tcgaaagcga cgagcccgcc
     7441 aggggccaga tgctgcgcca cgccggcgac gatcgcacgg taggcatcga ggccgtcgcc
     7501 gccgccgtcg agtgcagctc gcggatcgtg gttgcgtacc tcgggatcga gaccggcgat
     7561 ctcgcccgtc ggaatgtaag gcgggttcga gacgatcacg tcgaagcgcc ccgacacgcc
     7621 ggcgaaccag tcgctttcga tccaggcgac gcgatcattg aggccgagct gctcgccatt
     7681 ccgccgcgcg atctggaggg ctgccgcact gcgatcgacg cccacacccc tcgccgacgg
     7741 ccgctcgtgc agcagtgcca gcaggatcgc gccggagccg gtgccgagat cgaggatgcg
     7801 cagcggagcc gcagctccgg cgtcggcggt cgcctccaga accacatcga caagcgtctc
     7861 ggtatcgggc cgcggatcga gtaccgcggg accgatctgc aattcgaggc catagaacgc
     7921 gcgctgcccg agaattcgcc agaccggctc gcgggcgagg cggcgcgtga gtgnnnnnnn
     7981 nnncgccacc agccgatccg ccagcgcccc gaccggctcc tcgccccgcg cgacgagatc
     8041 gatccatgcg aggccgagaa ttcccgccac cagatgatcg gcgtcgcctt cagcaccatc
     8101 gatgccggcc gcggcgagct cacgcgcgat cagctgccgg gcttcagcgc gcgacattcc
     8161 cccgctgatc ggccgcgggc cacggctcat gcgcccgcct gctcgttcat ttcggcgagc
     8221 agcgtcgcct gatggtcggt gatcagcgcc tcgacgagct cgtcgagcgc ggtaccatcg
     8281 aggacggccg gcagcttgtg cagcgtcaga ttgattcgat gatcggtgac gcgcccctgc
     8341 ggaaaattgt aggtgcggat gcgctgcgag cggtcgcctg agccgacctg gcccttgcgc
     8401 tgcgccgcgc gctcgtctgc cgccttctgc cgcgtgaact cgaagagacg cgagcgcagc
     8461 acgcccatgg cggtacgccg gttctgatgc tgcgatttct ccgacgaggt gacgacgatg
     8521 ccggtcggca gatgcgtgat gcggactgcc gattccgtct tgttgacgtt ctgcccacct
     8581 ggcccagagg aacgcatcgt atcgatgcgc aggtcctcgg ccttgatctc gacgtcgatc
     8641 tcttcggctt cgggcagcac cgcgaccgtc gccgcggacg tgtggatgcg tccgctcgcc
     8701 tccgtctgcg gcacgcgctg cacgcgatgc acgccggact cgaatttgag gcgcgcgaac
     8761 acgccgcttc ccgtaatgct ggcgacaacc tccttcaagc cgccgagatc gttctcggac
     8821 atcgagatga cctcggtctt ccagccgcgg aggtcggcat agcgcgagta catgcggaag
     8881 agatcgcctg caaacagcgc ggcttcatcg ccgccggttc ccgcgcgcac ctccaggatg
     8941 atgctgcgct cgtccgcagc gtccttggga agcagctgga ccttgagtgt ctcgccaagc
     9001 tccgcaagcc gcggctcgat tgctccaatc tcctcgcgtg ccatgctggc cagttcgccg
     9061 tccgcacctg ccgccatctc gcgcgcgctg gcgagttcgc cttcaaggcg ctgcagctcg
     9121 cgaatgcttg caaccaccgg atcgagctcg gcgaactcct tcgacagccg cgcgaacgtt
     9181 tgcgggtcaa cgcccgagtt gagctcggcc tgcacggcgt cccagcgccc gacgagcttg
     9241 tcgagcttct cgggcgggat tgctgtcatg gtgacgctga tctcacgccc acgcgtggcg
     9301 agtgcggtgc gccgtctcga aatccttgat ccggccctcg tgctcgagcg tgatgccgat
     9361 gtcgtcgagg ccgttcagca ggcaacgctt gcggaaggga tcgatctcga acttgaacgt
     9421 ggcgccgtgg cggttcttct cggactgcgg gatcgagacg gtctgcgtct cgaggtcgat
     9481 cgtcaccgga tcgccggctt ctgccgccgc catcacctga tccacctcct cgggcttcag
     9541 gcgtaccggc agcatgccgt tctgaaagca gttattgaag aagatgtcgc cgaaggagat
     9601 tgcgatcacg gcgcggatgc cgaaatcatt gagcgcccag gccgcatgct cgcgcgagga
     9661 gccgcaggcg aaattttcac gcgagacgat catccgggcc ttccgccagg gnnnnnnnnn
     9721 nnnnnnnnnn nttgagcacg aagtctggct tctccttgcc ggcctcgtcg aagcgatgct
     9781 cgcggaacag gccgacgccg aggcccttgc gctccaccgt cttcaggtac tgcttcggga
     9841 tgatcatgtc ggtgtcgata ttgttgatcg gcatcggggc tgcgatgccg gagaggcgga
     9901 cgaacttgtc catggcgtcc tcgggtgttg gcggcgacag cgcatcggcg ctcgcggcat
     9961 gatctcgcct tggtcgtagc gcgctcggat tcgccagtca aacaggcgat ttactcgctc
    10021 cccgttccga cataaaaccg tcgcgagcca ttagcggtgc cggaccgggc accgccgcca
    10081 caccaagcca gagttcgggg acgacgcatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnagcc gttcgcgtcg ctgcgtgcca acgggcggaa ggtgcgcgag
    10261 ccgaagcgca cgctcgccgt cgtcgatcac aacctgacga cgggcgaccg caagaagggc
    10321 ttccctgacc ccgaaggccg gctccagatg gagctcctcg acaagaacgt gaaggaattc
    10381 ggcatcgaat actacgatgc cttcgacaag cggcaaggca tcgtgcacgt cgtcgggccg
    10441 gagcagggct tcacgctgcc cggcaccacg atcgtctgcg gcgacagcca tacctcgacg
    10501 catggcgcct tcggtgcgat cgcgcacggc atcggcacct ccgaggtcga gcatgtgctc
    10561 gccacgcaga ccttgatgca gaaaaaagcg aagaacatgc ggatcaccgt caagggcaca
    10621 ttgcccaagg gtgcgaccgc gaaggacgtg atcctcggca tcatcggcga gatcggcacc
    10681 gccggagcga ccggccacgc cgtcgagtat gccggtcctg tcatcgaggc gctctcgatg
    10741 gagggccgca tgacggtctg caacatgtcg atcgaagcgg gcgctcgcgt cggcctgatc
    10801 gcccctgatg agaagacgta cgcctacctg aagggccgac cgaaggcgcc gagcggcaaa
    10861 gcctgggacg aggccgtcgc cttctggcgg acgctcaagt ccgacccgga cgccaagttc
    10921 gaccgcgaga tctcgatcga cgtcggcaag ctcgccccgc tcgtcacctg gggaacgagc
    10981 cccgagcagg tcgtcgccat ctcgggcgtg gtgcctgacc cggcgaagat cgacgatccg
    11041 gcccgccgcg agaacgtcga gcgctcgctc gcctacatgg gcctcacgcc cggcacgagg
    11101 atgaccgacg ttaccatcga ccgcgtcttc atcggctcct gcaccaacgg ccgcatcgag
    11161 gacctgcgcg agnnnnnnnn nnnnnnnnnn ncaagaaggt ctcgccgaac gtctacgcga
    11221 tgatcgtgcc gggctcgggc gtcgtgaagg ccgaggccga ggccgagggc ctcgacaagg
    11281 tgctgaaggc cgccggtttc gactggcgcg agcccggctg ctccatgtgc ctcgccatga
    11341 acgacgacaa gctgaagccg aaggagcgtt gtgcctccac ctccaaccgc aacttccagg
    11401 gccgccaggg cccaggcggg cgcacgcatc tcgtctcgcc cgccatggcg gcagcggcgg
    11461 cgatcgccgg ccgctttgtc gacgtgcggc aactcggctg accctacccc cggagcgaca
    11521 cgcgtgccgc tgaccgccgc ctaccggccc gatccgcggc atttcacgct cggcgaggcc
    11581 ttcttcgacg aggtcggacc ggcgtcgttc ccgaaaacga ttctgcgcgt ccgtaatgat
    11641 cgctgggcgc gccgcatcgg gatcgaggcg ctctccgatg atgagtgggt tcgccatctc
    11701 gcgcgcttcg aagcgctgcc cgccaacatc cgcaagccgc tggcgctcag gtatcacggt
    11761 catcagttcc gcgtgtacaa tcccgacctc ggcgacggtc gcggcttcct gtttgcccag
    11821 atgcgtgact ccgtcgacga ccgcctcctc gaccttggca ccaagggctc cggccagacg
    11881 ccctggtcgc gtcgcgggga cggccggctc acgctcaagg gcggcatccg cgaggtgctg
    11941 gcgaccgaga tgctcgaagc gctcggcgtc tacacctcga agtcgctgag cctcatcgag
    12001 acgggcgagg ccctcacccg aaacgacgag ccctcgccga cgcgctcaag cgtgcttgtg
    12061 aggctcagcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nngcgcatgt ccgtcgccgc ggtcgccgag atgggggccg
    12241 agtggttcca ggccggtttc gtgcacggcg tgctcaatac agacaacacc gtcatcaccg
    12301 gcgagagctt cgactacggc ccgtggcgat tcctgccgac gctcgatccc gccttcaccg
    12361 ccgcctactt cgacgagacc ggcctctacg catacggacg gcaggctggt gcgctcttct
    12421 ggaacctgca gcgactcgcc gaatgcctgc tgccgctttc gagcgaggcg gctctggtcg
    12481 ccgccctcga gacgttcggg tccgcctatc agacggccgt agcgcgcttt acgctcgccc
    12541 gactgggcct cgccgatcag gacgggcgcg gccttgatga gatcgtgcga ccgttctatg
    12601 cggcgctcgc agccgcgaac atcccgttcg agcgcgcgtt cttcgatctc gtcggcggcg
    12661 cccgaaagga tcgcatcgcc gcgagcccgt accatatcgt ctatgacgcc gagccgttcg
    12721 caagcctcac ggcgctgatg cgcgactggc cgaaggcggc tggcctcgag gccgcactgg
    12781 cgcattccta tttcgctgat caggccccgc gcaccatgct gatcgacgag gtcgaggcga
    12841 tctgggcgcc gatcgccgag cgcgacgact ggcagcacct ccacaaggcg atcgcagcca
    12901 ttcgccaaat gggagctgcc cacgcgccgc tcattccctc gcgtgccagc cagccggcgc
    12961 atcggcgctt tgtagtcgcg gcccagcctt gaacgcttgc gccgccggat ttcgaacccg
    13021 gcggcgcggc agttcgtcct gctggcaaaa ggtttcggtc cgcaacgctg ccgaactggc
    13081 tcacttcatc ggcttcgggt gctcgaccgg ccggccgagc ttgatgctga ggcgtgcgcg
    13141 cacgacgtcg acgctggcat cgatgtcgcg atcgacgaag ccgccagccg cgccgaggca
    13201 cggacccgga caatggatct ctgtcccgag atcgatgtgc tgatactcga caccgaggat
    13261 cacgttgtgc gtcatcgcgt actcgatgcc gccaccgagg taccagccat tatggcgctc
    13321 attggccgag aaggtatgcg ccccggtcgc cagattgatc gccgtcgact gcacattcgc
    13381 cgacgcgaaa ccgccgccga tgaagaacag ccattgattg ttcggcgcaa atccgatgcg
    13441 cggaccgacc gtgaagatgc tgttcatgcg tacgagagag tcgaatgccg gattgttccc
    13501 gaacatgggc tcacgcgccc agtcgttgcc gaacacgctc gagccgctat aggccgcttc
    13561 gacgccgaca acgaggttgt tccactggtg ctggatgccg atatggccgc cgatcaggcc
    13621 accgtcgtcg ctcagcgaat aggcctggtg cgctgcgccg aggatcggcg gattgaacgc
    13681 ccaatcgaag tccttgcccg cccagccggc attgatgccg aaatagactc cgccccagtt
    13741 gtgatcgtgc gccaccgcag gtgccgtccc cgatccggag gcaaccgccg cgatggcgaa
    13801 cgctgcccca accgacttga accacttcat ttcggtactc tcctcgacta tcggccgcgg
    13861 agcctgatca ccgtccccaa gatcaagcac gcactcggcc cgaacgctcg aaccaacctg
    13921 taccgccaca tttcaatgcc cctcgatcga agcgcacgag cggctcgctg gtcaatcgtt
    13981 ctcgaagacc tgtgtgtcgc ttcgtgccgg ttaagtacct gcaagagcac aattttgctc
    14041 acgcctggac tannnnnnnn nnnnnnnnnn nnnnngcctg ttacaggcgc ctgcactcta
    14101 tgacccgctc gccaacccgg aagccgccaa ggccgccatc atccgccagc gcaacaccga
    14161 cgccgacgcc aagacgagga gcacgccgtg gcccgcaccc cgaccgccct gaagccccga
    14221 ccaaccggca ctgcgcacgc catgacaccg ccgccgcggg ccgcgactgg cgccgacgcg
    14281 cggcgcatgt tccgtaaggg cgagttcaca ggtgccactc acggcatcgc gcccggctac
    14341 gtgcagggca acctcgcgat cctccacaag gatctcgcgc ccgacttcgt gcgcttctgc
    14401 cagctcaatc cgaagccctg cccgctgatc ggcatgtcgc cgaagcccgg cgatccgacc
    14461 atccccgagc tcggcgccga cctcgacatc cgcaccgacc tgccgaagta tcgcttgtgg
    14521 cggaacggcg aactcaccga cgagcctgcc gacatccgcc aagcctggaa caacgatctc
    14581 gtcgccttcg tgctgggctg ctcgttctcc ttcgaggagg cgctggtcga gcaaggcatc
    14641 gagcttcgcc acatggcctg cggctgcaat gtctccatgt ggcggaccag catcccgtgc
    14701 acgccagtcg gcccatttca tggtccgatg gtcgtctcca tgcgcccgtt gaaacccgtc
    14761 gatgcgatcc gcgccgtgca ggtcacctcg cgctttccct cggtgcacgg cgcgcccgtc
    14821 cacattggca agcccgagct gatcggcatc cgcgacatca acgagcccga ctacggcgat
    14881 gccgtcgagg tgcgcgacga cgagcttccc gtattctggg cctgcggcgt caccccgcaa
    14941 gccgtgatcg cggccatgaa gcccgaattc tccatcaccc acgcgccggg atcgatgctg
    15001 atcaccgatc tcctgaacag ccggctcgcc gtcatctgag gctccgatgg atcttgcaat
    15061 caaggggaag gccgccctcg tcacgggcgc cagcaccggc atcggccgcg gcatcgcgct
    15121 ggccctcggt cgcgaaggcg tgcggctcgc cataaccgcg agacgggggg agctgctatc
    15181 ggaggtggcg cgcgaaatcg aggctgcggg agcgccgccg cccgtcacaa tagtccagga
    15241 cgtgatggca gaggatgctc cgctgaggat cgccaaggcc gccgtcgcag gcctcggcgg
    15301 cgtcgacatc ctcgtcaaca acgcgggcgg cagccggcaa ttcggcaagg acgcgaccga
    15361 ggcgcagtgg accgaggcga tgacgctcaa cttccatcgc ccgcgccagc tcgcccacca
    15421 gctgctcgac cagatggtcg cccgcaagtg gggccgcatc gtcaacatca cgggaaagtc
    15481 ggaacctgag ggcgtcaacg gcgccttctc ggccaaggcc ggcatccatg cctgggccaa
    15541 ggggctctcg cgcgaggtcg gcaagctcgg catcacggtc aattgcatcc cgcccggacg
    15601 catcatgagc gagcagatcc gccgcaatta cactcccgag taccggaaat ggcaggccga
    15661 gcacgagatc cctgtcggcc gctacgggga gcccgaggat ctggccgcgc tcgtatgctt
    15721 cctggcgtcc ccgctcgccg gatacattac cggcaccgtg atccccgtgg atggcggcct
    15781 ccgccgctac cagttctgag cggcgccatc ctgcgcttgc gccgcttctg ttgtatcttt
    15841 gccgcgccga gcgtccactc gtgacgttgt gcacggctga tcgcctccgt ctcgtcccat
    15901 ttctacggga tttgcctgta gacggggcgg atcgccctaa aaggtcgagg gagacgaaag
    15961 cgcgatgcgc ggatcgaaga gactggctca gggtgctgca tttgcgctgg cgctgatggc
    16021 aacagccgcc gtggcgcagg atacgaagaa gggcgcgccg ccaggaagcc ccgtcggcgg
    16081 cacctggtcc gcgccgaagg tcgttccaga cgcgccgacc ggagggcgca ccttcgagcc
    16141 caaggagatc gaggcgatca agcgtgtcag cgagtacttc aacgcgctga ccacgctcaa
    16201 gggccgcttc gtgcaaagtg tcgccgacaa ggagaagcga ccgatgcgcg gacgctttta
    16261 cctgcagcgg cccggccgca tgcgcttcga gtacggcgcg ccgagcaagc agctcgtcgt
    16321 cgccgacggc aagatgctgg cgatccagga tctcgacctc gagaccgacg accagatcgc
    16381 gctcgatcag accgcgttcc gcatgctgct gcggcccgac gtcgacctca tccgcgacgc
    16441 gcgcatcctg gatctccagg aggccgacga cgtgatcatc ctgacgctcg tggacaagaa
    16501 cccggacgcg gccggcaaaa tcaaactctt cctcgtgaaa aagccaggcc tcgagctcaa
    16561 ggagtgggtg acagtcgacg cgcaaggcat cgagacgcgc gtcgaggtcg gcgagctcgc
    16621 gttcgacccg cagctcgtga agggcgaaac gatcgagccg aagctgttcg tcatcgagcc
    16681 gatctcactc aagaagaaga ccgggcagta agacgagcgg ccgcagcccg gacgtccctg
    16741 acattagcga cggcgccgga gcaaccgctt ggcgccgttt gctattccga gatttgcccc
    16801 aaaagaggcc gcgcgccggt gctatagcta tcgacgaggg cgcaacgccc tcgattggat
    16861 cgcggcgtgg gcagagaggc aacgtggacc ggcgcggcgg agcgatcagt acccgcattg
    16921 tcaatcgcgt gctggccgca ctcgcgatcg ccggcctcat gatagccggc gcgccgatcg
    16981 tcgctcagcc gagccgcccc gatctcggcg ccctgaaagc gcttttccgg cggcccgacc
    17041 gcattccgca tcccgtggat gcgccattga cgcccgagcg gatcgcgctc ggccgtacgc
    17101 tcttcttcga tccgcggctc tcttcgaccg gcgttgcctc gtgcgcgagc tgccacgatc
    17161 cgcgccgggc cttcacggat ggcggcggtc gcggtgtgac tggacggtca accaagaacg
    17221 ccgcaccgat gctctggaac ctcgcctggg caagggcgct cttctgggac gggcgcgcac
    17281 cgacgctgga agctcagctc cgtggaccga tcgaaagccc ggacgagctt ggaacgagcc
    17341 ttcaggatat cgcgcgccgg ctcaacgcgg atacggcact gcgcacctcc ttcgctcggg
    17401 cgtttccgga aaagaccgag atcgacagcg cgttggtcgt cgccgcactc gccgccttcg
    17461 agcgctcgct ggtctcgccg ccgacccgct tcgaccagtg gatcgccggc gacagcgtag
    17521 cgctgacccg tgccgagatc gacggcttca cactcttcac cggaaaggcc ggttgcagcg
    17581 tctgccacgg cggctggcgc tttaccgacg ataagcgcca cgatatcggc cggccgatca
    17641 ccacggaacg cgaagaccgg aaccccgccg tgaagacgcc gacgctccgc gagctcacct
    17701 ggaccgcacc gtacatgcac gacggctcgc tgccgacgct cgagagcgtc gtcgatcact
    17761 atgccgatcg catcatcgat cgggcgaccc tcgcgcccga acttcgacgc cgccaaccgc
    17821 tttcgcagac ggagcgaagc gccctcgtca ccttcctgat gtcgctctcg ggcgcaagcc
    17881 cgccgccgct ccccgactga ctggacttga ggcatcgaac cgaccacttt gatgccgggt
    17941 caggcatttg ctatcgtgcc accgcaacgc cttggcgggg atcaggcccc gcacgtctcg
    18001 ggcgttgagg gagagaaaca tgcatcgcag aaccttactc aagggcctcg gccttgccgc
    18061 gggcaccatc gccgcgccgg ccgtgctccg cgcgcaggcg ccgatcacgc tcaacggcgc
    18121 cgttcagttc gacgacaacc acgccttcac caaggcgctg acgcggttcg gcgagctcgt
    18181 caacaagtac tacggcaagc cggtcaattt cacactgcac aagaactcct cgctcggcct
    18241 cgaaaagcag tatttcgagt acatggcgca gggcaaagcc gtcgacttcg cgatcgtctc
    18301 gccggcgcac atgtcgacct tcgccaaggc cgcgcccttc atcgacgcgc cgttcgtgtt
    18361 ccgcgacatc gcccacatga acgcggttgt cggcaaggac ctcctgaagc ccatcgcgga
    18421 tgaagtcgcg cagaaggccg aggtgatgct gatcgatcag gtcacgnnnn nnnnnnacaa
    18481 ggtgatcacc aatctcgccg agctcaaggg attgaaggtc cgcgtgcagg gtgcgccgat
    18541 ctggtcgcgc actttcacgg ccgccggcat gtcaccgacg gtcatcgcct acaacgaggt
    18601 ctacaacgcc atccagaacg gcgtgatcca ggccggcgag aacgaggcgg ccggcatcga
    18661 ggcgatgaag ttcttcgagg tcgcgccgaa catcatcctg acgcagcacg cagtatcgat
    18721 ccgccccatc tgcttctcgg tgaagatctt caagacgctg ccgaaagagc tgcaggacgc
    18781 gatcgtcaag gccggcaagg aagcgggcga cttcggccgc ggcctcgaga gctcggagga
    18841 cggcgccaag ctcgaagccc tcgagaaggc cggccgcttg aagcgcatcc ccttcgccga
    18901 ccgcgccgcc atggccaagc tcgtcgagcc cgtcatggac gcctacgcca aggagatcgg
    18961 cgccagcgag atcgccgcca agatcaaggc ggcgtgagcg ctcggacgtt tgcggcccag
    19021 gcgcgatgtg cctgggccgc tacattgcag ctcagggccg cgcagacggc tccatgcgtc
    19081 tcaactcgtc agggcgatga attcaaccca ggagccgtat gcggccacct tcgcgaacgt
    19141 gacaacgatg ggcgcgacag ccctcggacg cgcttcgcga acccgccgtc cccgtcaagg
    19201 ccggcccgtg tcgggttcgg cctagcctcc tgcggtccca tgcggctcct ctcgctatgc
    19261 tggttcgccg cctgacacct ccgcggcggc cgtcgccatn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnncgt cgatgctccc gcatagaggc gccgcaattg gacagcgccc
    19381 cggggagacg ccgcacgtcc gggcaggcct ccaggcgtgt tgaccagccg ctggtcgatt
    19441 gcgtaacgtc gattgacctg tttcgtggcc tgagcattgc gcctcgccgc gcaccacttt
    19501 accaacgatc tgaccaagtt cggcagtcca ggaggaaatc gtgctcaccc gcaggcaaac
    19561 tctcataggc accgcttcga tcgtgccggc cacgcttgtg gggcgagcgg ctgccgcgca
    19621 aggcttgtcg aaccttcaga ttttcgtgcc ggcagcccct ggaggcggtt gggacgcgac
    19681 cgggcgcgcc atagagctct cgatgaagac cgacggcatc gtgaaggact tcaggttcga
    19741 gcacgcgccg ggcgccggtg gtgcagtggg actgccgaag ttcctgcaga ccaagaaggg
    19801 ccagggcgat gcgctgatgg tcggcggcat ggtgatggtc ggttcgctga ttgccaacaa
    19861 gagccccgtg tccatgaagg acctgactcc ggtcgcgcgg ctgacgggcg aagcgaacgt
    19921 gctcgtcgtg ccggcagcga gcccaatcaa ggacatcaag ggctgggtcg acgcgttcaa
    19981 ggccgacccc ggcaagatgt cggtcgccgg cggatcggcc ggcggcaccg atcacatcct
    20041 catgggcatg atcgccaagg ccctcggcaa gaccgcgaag aacgtgagct acgtcgccta
    20101 tgcgggcggc gggcctgccg tggcggccat tctcggcaat caggtcgcct gcggcacatc
    20161 aggcgtcggc gagttcgccg agcatgtgaa gagcggcaag atgcgagcca tcgccgtctc
    20221 gggcgcgagc cgcgtggcag gcatcgatgc accgaccctg gtggaggccg gcatcaacgt
    20281 cgagttggtc aattggcgag gcgtgttcgg cgccccgggc ctaacgtccg accagaccaa
    20341 gacgctcgtc gacaccgtcg acaggatggt caaaggcccg acatggcagg cggagctcaa
    20401 gaagaaggac tgggccaacg tctatctcgg cggcgccgag ttcggcaaat atctcgacgc
    20461 tgagatcggc cgcattacca gcatcctcaa ggatctcggc ctcgccacgt gaggcggatc
    20521 aaccgggcac cccgcggaga agccccgcgg ggtgctccaa ttcgattggc cgcgagagat
    20581 ctcatgcaca agaatgtggc gtggcaggaa accgtgctcg gtctcttcgt gatcgcactg
    20641 gcggcggtgg tcggctggca gacgatggtg atcccggaga acgcgatcta tgcaaagatc
    20701 ggtccgcgct tcttcccctg gatcgcaacc gcgctgcttg tcgtcatggg cgttctgctg
    20761 accgtgcaag gcctccaggg cgggtgggag cacgagctcg acgatgatcc cgatcttttg
    20821 gccgtgggat ggctcgccgg cggacttgtt ttcaacgtgt tgttcatagg cggcgccttc
    20881 ggcttgccgc gcctcggctt catactctgc tcgaccgtgc tcttcacctg cgtcgcccgt
    20941 gcattcggca gccggcagcc ggtgcgcgat gccgcgatcg gtttcctgct cgcgctcgtc
    21001 tgctacgtcg ggttcgatcg cgtgctcggc tacaagatcg gctcgggtcc aatcgagggt
    21061 ctcatctgag atggaaaccc ttggcctgct ccttcaaggg ttccagacgg cgctaacgcc
    21121 ggtgaacctc gtttggtgct tcgtcggcgt gttcgtcggc accgccatcg gcgtattgcc
    21181 tggcctcggg cccgcactca ccatcgccct cctgctgccg ctcacctaca aggtcgacgc
    21241 cacggccgcc ttcatcctgt tcgcgggcat ctactacggg gccatgtacg gcggctcgac
    21301 cacgtcgatc ctgctcaata cgccgggcga aagcgccacc atcgtgacag cgatggaggg
    21361 aaacaagatg gnnnnnnnnn ncgccggcgc cgccctctcg acggcggcca tcgtctcgtt
    21421 cgtcgccggc acgatcggta ccatcggcat cacgtttctt gccggacccg tcgtcgaatt
    21481 cgccctgtgg ttcggaccgg ccgagtattt ctcgctgatg atcctggcct tcgtcacggt
    21541 ttctgccgtg ctcggtaatt cggctgtccg cggcttgacg tgcctcgcgc tcgggatttt
    21601 cttcggtatg attggcatcg acctgcagac cggccagccg cgcttctggt tcggcgtcac
    21661 cgagctctcc gatggcatca acgtgatcgt cgtcgcggtc ggtctctttg ccgtcggcga
    21721 gaccatctac ctcgcctcca aacgccgctc cggcacctcc gacgaggtac tcgaggtcaa
    21781 ggacagcatc tggatgtcgc gccaggactg gagccggtcc tggaagccct ggctgcgcgg
    21841 aacggcgatc ggattcccga tcggcgccat gccggccggc ggctcggagg tgccgacgtt
    21901 cctctcctac tacaccgaga agaagctctc gaagcacccc gaggagttcg gtcacggcgc
    21961 catcgaaggc gtcgccgggc ccgaggccgc caacaacgcc tcggcggcag gcgtgctcgt
    22021 gccgatgctg gcgcttggtc taccgacgtc tgcgacggcc gcgatgatgc tctcggcgtt
    22081 ccagagctac ggcatcacgc ccgggccgca gctcctgcag cagcaggcca atctcgtgtg
    22141 ggcgctgatc gcgagcctct atgtcggcaa cgtcatgctg gtggtgctga acctaccgct
    22201 tgcggcgctg tgggtgaagt tgctcaagat ccccgagccg ctgctctacg gcggcatcct
    22261 cgtgttcgcg accatcggca cttatggcat tagtcagagt accgtcgacc tggtgctgct
    22321 ctacatcgtc gggttcctcg gcttcctgat gcgcaagttc gacttcccca cggcgccggt
    22381 gatcatcggc atgatcctcg gaccgctcgc agagcagaac ttccgccagg ccttgaccat
    22441 cagtcagggc gacatgacag tctttttcac gcggcggctc tcgctcgcgc tgctcctgct
    22501 cgcattgctc gccctggtcg gaccgcgatt gtggcagctc gtacggcgac ggccgagctg
    22561 atcgactaaa gcctcatacg aacccacccc tgccttaccc cgggctcgtc ggcagctcgt
    22621 cgctcgcttc ggcgaggcag cggttgaact cggcgccgag gatgatgatc atgctcgtca
    22681 cctggaagaa gatgaaagcg ctcatgagct gcgtgagtcc cgcgtagaag cgcgagtagt
    22741 cgttgaggat caggtagcgc gagtagaagg ccgcaaccag cagccagagc gcgatcgaca
    22801 gcaccacgcc aggccaaacc tcgcgcacct tgcgccggcc ggccgcgagc cagaggtgat
    22861 agacgaagag ctggcccaag aggatcagcg tgacgaccgt gtagcggaag cccacgttga
    22921 aatagccctg ctcgatcagc caatcgagcg tcggcgactt ccaggtccac acgatcactg
    22981 gaccgatgat cacccccgcc gccagcgcca acgcgccgag cgcgctcgcg aacacgatga
    23041 gcgagctctg cagcaggcac cagagatagg agcgtgtctc ctgcacgtgg taggccgtgt
    23101 tgagcgctgc acgcaggctc tcgatcgcgc tcgtcgcgaa caggagggcg acggcggcgc
    23161 cgatcgtcag caggccatac tggctctgcc ccatcaccgc gaccacctgc ggcgaaagca
    23221 cctcggcgac cggcgccggc agtacggcga acatgatcgc cannnnnnnn nnnnnnnnnn
    23281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cttgcggacg
    23401 gcgcgcccga gcacggaaag ctctctcatc gtggcgccgt tcctctcttc atgtggcggc
    23461 tcctcccgca atcgatccgc caacgcataa gctagcgccg cttggtctct ccccgtccaa
    23521 ttggtgtcaa gcgcggaatg ccgggtgcgt ccgaaggacc tcgccgacct tgcggatcga
    23581 cctttgcgcc gtcgcgtctt tgcgcttcac tc
//
LOCUS       DAORNH010000028        23420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1671_length_23420_cov_2.655510, whole genome shotgun sequence.
ACCESSION   DAORNH010000028 DAORNH010000000
VERSION     DAORNH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23420
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_23420_cov_2.655510"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    378..402
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <403..1200
                     /gene="dapA"
                     /locus_tag="PK264_04880"
     CDS             <403..1200
                     /gene="dapA"
                     /locus_tag="PK264_04880"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRD75258.1"
                     /translation="PVGTTGESPTLTHEEHKRVVELAVKTARKAKRYVPVIAGCGSNS
                     TAEAIDYTKHAKKVGADAALHVLPYYNKPTQEGQYQHIKAISDAVDLPIFIYNIPGRT
                     NIEMSVATMARCFKDLKNVIGVKDATAKIDRVTLQRLACKRPGQEFIQLSGEDATALA
                     VIAHGGHGCISVTANVAPRLCAEFENACLSGDWKTALALQDRLMPLHDALFCETSPGP
                     VKYAAFRLGLCSAEMRLPMAPIAEESRKRVDAALVAVGLSNLAKAAE"
     gene            1319..1795
                     /gene="smpB"
                     /locus_tag="PK264_04885"
     CDS             1319..1795
                     /gene="smpB"
                     /locus_tag="PK264_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRD75259.1"
                     /translation="MAAKKDENRKTVAENRKARFEYHIEETIEAGIELKGTEVKSLRL
                     GQANIAESYASSEAGGIMLINSYIPEYKMAGAFFQHEPRRPRRLLLRAREIHKLTIAI
                     ERQGMTMVPLELYFNQRGFAKLRLALAKGKKTEDKRADIKKRDWQREKARLLRDKG"
     gene            complement(1811..3409)
                     /locus_tag="PK264_04890"
     CDS             complement(1811..3409)
                     /locus_tag="PK264_04890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HRD75260.1"
                     /translation="MERFARLLDTLAFTPARNGKLAAMEEYFRAVPDPDRGYALAALT
                     DGLPIRVPIRRILADLIAARMDPVLYKLSRDFVGDTAETVALLWPEPDSRDARSPAPI
                     LADLIASLATTPSSSLGRFIAGTIDRLDSRGRWAFLKLVGGAPRVGVSARLAKTAAAA
                     IGAKEVAEVEEVWHALAPPYRELFAWLEGHAPRPDVLGKPVFRPLMLAHPLEDSDLPA
                     IKPSDFVAEWKWDGIRVQVAAGGGATRLFSRTGDDISSAFPEIAEAFAGREVTADGEL
                     LVVRGGIVAPFNELQQRLNRKAVTRRQLADHPAHVRLYDVLTIGERDLRPRPLLERRR
                     ELEAWHSAAPPRLTDLSTIVPFADLDALRRLRDGARDAGIEGLMLKRADSPYLAGRPK
                     GHWYKWKRAALTLDCVLMYAQRGSEKRSSLYSDYTFGVWREGERGPDLVPVGKAYSGF
                     TDEELVRLDRFIRANTVETYGPVRAVRAELVLEIAFDAIQRSARHKSGVAMRFPRVHR
                     IRWDKPAAEADTLATAKALIENSA"
     gene            complement(3413..4420)
                     /locus_tag="PK264_04895"
     CDS             complement(3413..4420)
                     /locus_tag="PK264_04895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HRD75261.1"
                     /translation="MPAVSTWLYPTPSGLYCEPGGFHIDPTRPVDRAVITHGHSDHAR
                     AGHGAVLATRETIEIMKVRLGENSAGAFQVITPGETLRLGAVDVRLAPAGHILGSVQV
                     VMEHAGTRAVASGDYKRASDPTCAPFEPVTCDVFITEATFALPVFRHEKAEHEAARLV
                     ASLAREPRRTHLLGVYGLGKCQRMLKLVRSAGYDRPIYLHGALVELTKLYQNFGQDFG
                     DVRPVATATPAELEGALVLCPPSSLSDRWSRRFGDAVTVFASGWMRVRARARQRGVEL
                     PLVVSDHADWPELLSTIVETEASDVWVTHGREDALVHQLASMGRSARALALVGYEDEA
                     E"
     gene            4600..6540
                     /gene="selB"
                     /locus_tag="PK264_04900"
     CDS             4600..6540
                     /gene="selB"
                     /locus_tag="PK264_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRD75262.1"
                     /translation="MIVGTAGHIDHGKTSLVKALTGVDTDRLPEEKARGISIELGFAY
                     TVLEDGQSLGFVDVPGHERFVHTMLAGATSIDFVVLVVAADDGVMAQTREHLSILGIL
                     GLSDGVVALTKCDLVDEARLAAVEAEIRVALAASSLRDADILPVSSVTGAGIAGLRTR
                     LAEEAMSRPARPGRGQLRIAVDRAFSIAGAGTIAAGAITGGRITVGDIVVALPSGAEC
                     RVRSLHVNGRAATVATYGDRCALNLVGADLSRGDVARGDWLVAPDARMATARVDARVE
                     LLASEKRPLATWVPVFVHVGTSAAPARIIVLSSEKLLPGGNALVQLVLPKALPLRHGD
                     RVVLRDIGAERTIGGGMVVDPQPPSRKRRTDGRLAMLAALEEIVAAEALSRLAEVEAG
                     LVDVHAFAAGRGLTPAELVTALAEAGLVAVGCATEGQVPRFATSESRLDALAMSFETA
                     LGASHAREPALAAIPLQRVRLAMSPRIAPDECEDLAGHLVAAERVARLGGGVRLPNHK
                     SALADADMRVWDKLRPHLLAKPRHPPTLREIGLAIKVEVQGLRRVAKQLARLGELVEI
                     GADRYFARSAVIELAEVVAELGRTRALGVFTAADFRDAAGTGRNVGIAVLEYFDRVGI
                     TVRRGDERRPGKPAAEVLARRP"
     gene            complement(6555..6968)
                     /locus_tag="PK264_04905"
     CDS             complement(6555..6968)
                     /locus_tag="PK264_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD75263.1"
                     /translation="MKIYRPNARPTKTAPAKWFVGGVWQDELVPAPDAPSRLRVNKVS
                     FMPGGRTNWHTHPVAQVLHVLSGVGRLQEAGKPLQVLNPGDTAVIGKDVKHWHGSAPG
                     HSFVHLALTECDAAGESATWLEPVSDADYNKTPES"
     gene            complement(7053..7802)
                     /gene="pxpB"
                     /locus_tag="PK264_04910"
     CDS             complement(7053..7802)
                     /gene="pxpB"
                     /locus_tag="PK264_04910"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRD75264.1"
                     /translation="MAPHDTAALLTLGDQAVGVEFGDTVDPVLSGKVLALDAAILAGV
                     KAGTLGPGIVETVPTYRSIAVHVDPFVADIDALATAVLEMARSIEPLVAAARRWRVPV
                     VYGGDYGIDLDATAARLGMSAAELIERHSAPVYRCYMIGFLPGFAYLGGADPRIAIPR
                     RDSPRLKTPAGTISIGGIQAGIQSVEAPSGWHLLGTTPVRNFMHGRDPAFLVCAGDEV
                     EFYPIAASEFEGLSRRAAAGEPVAELIPAER"
     gene            complement(7805..9112)
                     /locus_tag="PK264_04915"
     CDS             complement(7805..9112)
                     /locus_tag="PK264_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD75265.1"
                     /translation="MLTAVLTLFAVLFGALSAGLWIGLTLALTGVVMLGLFKSVPLDK
                     LLAQYTWNILTTQELLALPLFIVMGEILFRTRLSASLFNGLAPWAGLLPGRLLHVNVV
                     GCSIFAAMSGSSAATTLVVGRLSLAELLKRGYSRDIAIGSLAGAGTLGFLIPPSNILI
                     IYGVLGEVSILKLFTAGIIPGLLLALAFMAWIMIHTRLRPSLVPASEHALGSIPWADR
                     FRALKDLAPAVFLIVCVLGSMYRGWATPSESAAVGVLGAVIVTAIQGTLTFENAKSIL
                     MGAVTTCAMIALIVLGASILASSIAFLGIPKAIADWVASLGLSPFMLIAALVAFYLIL
                     GCFLDGFSMIVMTLPIVLPIVKAAGFDPIWFGIFLVMVVEMAQITPPVGFNLFVIQGL
                     TEDSLGYIARVTMPYLFIMVGFTLLLAIFPEIVMYLPNRVTKS"
     gene            9452..10657
                     /locus_tag="PK264_04920"
     CDS             9452..10657
                     /locus_tag="PK264_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HRD75266.1"
                     /translation="MSALRRRVYEIIELAREGDGVSRLVDLGLVILILLNVAAFVLET
                     VPEFEVAYRSQLYWFELFSVAVFTIEYTARIWSCVEMPFLNMRAPWLSRLKYATKPYL
                     LIDLLAILPFYLSFVVPIDLRVLRVFRLFRILKLARYSPTMHILLRVLAHERRTLVGT
                     FLLAMTLLLFVATSAYYAERIAQPEKFGTIPDAAWWAIATLTTVGYGDVAPITPLGKI
                     IGGMAMICGIIVLALPIAVIATGFSNEIGKRDFVVSWPVISRVPSLACLRDDEIDAVT
                     PFLAAQFMDPYREVIAPEDRADAMFFVVRGSVRLRTNAGETVFRVGDFFGELAMLEGR
                     SHTHSYRTTEPTRLLRLAKDDFEFLSESHPAIAHQIMSVARKRRTAREAGEADPAAGT
                     STPAADHSA"
     gene            complement(10703..11062)
                     /locus_tag="PK264_04925"
     CDS             complement(10703..11062)
                     /locus_tag="PK264_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75267.1"
                     /translation="MRPTAIAITSALVAVSGAITAAPVLATDFKIDCASLEEHRSPKI
                     GPAAKLTIENERATDIEVKVIRNNGADISLGAVKAKSKREVTTRMRYVFSLHDAKGGK
                     CIQGVRMTSDNITIVVK"
     gene            complement(11255..11590)
                     /locus_tag="PK264_04930"
     CDS             complement(11255..11590)
                     /locus_tag="PK264_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRD75268.1"
                     /translation="MAKAAAKAAPAKAAPAKAKTTTVTLRHLGTALADAHSIPKKQAY
                     ALLEDMVAMVRTHLKKGARIRMGGLGILQVKKRPARLGRNPRTGEQIKIKASKKVAFR
                     AAKELKEAV"
     gene            12008..13123
                     /locus_tag="PK264_04935"
     CDS             12008..13123
                     /locus_tag="PK264_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRD75269.1"
                     /translation="MTSTIITGLSTTLFRVPWSGEPPANGIMPPAARELLALEVRTKG
                     GLTGLGYLQPLSGGIETLDQCLAEMIAPKIIGRDATEVEGIWQALWKSTYWLGRGGIA
                     TFAQSAVDIALWDIVGKKAGLPLHRLWGSMRTEVPAYGSGCWRGLGRDGMIARARHFT
                     SKGFKAIKMQAAHIRPWREDVRNVHAMRDALGPDVEIMIDVNMGWDAATAIQAGRHFD
                     EADIYWLEEPVVCEDFDGYQRVAAALKTRVVGGESHFTRSDLRPFFRTPTLPILQPDP
                     MRGGLTELRKIAAVADTWGLQIAPHLFHELTVHVLASIPNANYLEYMDWNDDLWVTPA
                     IPKDGMLRAPEAPGHGLAFKPEILKSCRFGGRETSRG"
     gene            complement(13136..13951)
                     /locus_tag="PK264_04940"
     CDS             complement(13136..13951)
                     /locus_tag="PK264_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRD75270.1"
                     /translation="MADESRQQRRARQRDWLKIGRAAFDDGLRPEPRREEVYGAALVL
                     RAKLKERGNVTRASEAAGLAQKLAEASRASFAAKEAVACAKGCAHCCHNYVGVIPPEV
                     FAIARAIRQGARQGNTFLEPAAVLERGRATRDKSPEERIGAKIACQLLGTAGACSVYA
                     VRPLVCRQTASFLVEACKSELEGDRSLMTVPNRHMAAGSNAHLALLAAMKSVGMPTIG
                     YELAAALEVALTTPDAEARWLAGDTIFSALTNIIRRPAAVEGVVARIADEVGG"
     gene            complement(13976..14965)
                     /locus_tag="PK264_04945"
     CDS             complement(13976..14965)
                     /locus_tag="PK264_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRD75271.1"
                     /translation="MNADGRVLSGATRPLQKFEDPDWTAKGERRATVPFARLQTLWFN
                     TGTLCNITCASCYIESSPKNDRLVYLSAAEVVAFYDEILAKRLGTKEIGLTGGEPFMN
                     PDIIAIIEEGLERGFEVLVLSNAMQPMQRPRIKKELLRLNSAYGRQLKLRISLDHFTR
                     ELHERERGPKSWEKALAGIDWLSKNGFTLAIAGRTCWGESEPAARAGYAELIAARRWQ
                     IDANDHAALHLLPEMNEKAEVPEITNACWGILNKRPVDVMCATSRMIVKRKGADRPTV
                     LPCTLIPYQPQFDMGPTLTEAGAADGGMFDKGAVKLCHVHCAKFCVLGGGSCS"
     gene            complement(15164..15493)
                     /locus_tag="PK264_04950"
     CDS             complement(15164..15493)
                     /locus_tag="PK264_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75272.1"
                     /translation="MFLIRTAFWLAVVVMLLPTDEKKQAQLKAAAWDAAYRAATFCDR
                     NGETCAQAGQIWVSFKQKLEFGARLTSDLASEYVFKATRRHRPRRWRRAPVLSIPIRA
                     ARSCRPT"
     gene            complement(15625..16005)
                     /locus_tag="PK264_04955"
     CDS             complement(15625..16005)
                     /locus_tag="PK264_04955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD75273.1"
                     /translation="MRMINPETIAKPASSYSQGIVHGAGPRLVISGQVGVAADGKIES
                     GIEAQMRRAWSNFTAVVKAAGFEPHHVVKVTVFCTLPGQVGLFRRLRDEALLGHKATA
                     TYLQVAGLATPEYLVEIEGEAVKD"
     gene            complement(16041..16370)
                     /locus_tag="PK264_04960"
     CDS             complement(16041..16370)
                     /locus_tag="PK264_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75274.1"
                     /translation="MITGKLAWFAVAAGLIVVAYILRRWAQRHDLKSMATDAAWAAAR
                     KRGMPEGTELQKRIQAYSDEPSNAERARMATVDIGKHVVGRWLAILAWIIALSGFAIA
                     FFAYLWG"
     assembly_gap    16444..16461
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16596..17984
                     /gene="miaB"
                     /locus_tag="PK264_04965"
     CDS             16596..17984
                     /gene="miaB"
                     /locus_tag="PK264_04965"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRD75275.1"
                     /translation="MVGKKSVSNGEQAMPGGKKVFIKTYGCQMNVYDSERMSEALAPA
                     GYAETSEMADADLIVLNTCHIREKAAEKVYSELGRVRDVKSAREARGRSTIVTVAGCV
                     AQAEGAEIIARAPVVDLVVGPQTYHRLPDLIGRAGSSSGGLVETSFPDEDKFEHLPSR
                     EGARRRSAAAFLTVQEGCDKFCTFCVVPYTRGMETSRPVERIEAEARRLVEQGVREIT
                     LLGQNVNAFHGPGPDGSAWSLAQLLRRLAEIEGLERLRYTTSHPRDMSADLIAAHASV
                     AKLMPYLHLPFQAGSDRILAAMNRKHTAQDYRELVCRIRAARPDIALSTDIIVGFPSE
                     TEDDFRATLTMVEEIGFAQAYSFKYSARPGTPASTIEEQVPEGVKAFRLLRLQELLES
                     QQRRFNAGCVGKTFPVLFERPGRHSGQLVGRSPYLQPVHADAEPGLLGRIMPVEIPAA
                     GPNSLSGVIKAA"
     gene            18094..19092
                     /locus_tag="PK264_04970"
     CDS             18094..19092
                     /locus_tag="PK264_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRD75276.1"
                     /translation="MRAQGGSGKAARPELAKIVVPFEDNRLVAELLGEHDAHLALIEE
                     RLGIDGHAHGNIVTLAGPPDAIEIARFALETLYGRLRRGERIAAGDLDGLIRHGESAV
                     GPAVAGGARDLAGMAQIRTRRRIVTARTPMQSRYIEALERHDLVFGIGPAGTGKTYLA
                     VAFAAASLERGEVERIILSRPAVEAGERLGFLPGDMREKVDPYLRPLYDALYDVLPPE
                     KVERHLETGVIEIAPLAFMRGRTLANAVVILDEAQNTTTMQMKMFLTRLGENAKMVVT
                     GDPTQIDLPSGQVSGLIEAVRLLEGIKGIAAIHFQAADVVRSDLVARIVAAYEGRK"
     gene            19089..19595
                     /gene="ybeY"
                     /locus_tag="PK264_04975"
     CDS             19089..19595
                     /gene="ybeY"
                     /locus_tag="PK264_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRD75277.1"
                     /translation="MTRPDGQRPGAALAIEIVEESGNWAVVPEAGALIAQATDALARH
                     PAIAGRLPAAATIVLASDADVQVLNRTWRGKDKPTNVLSFPAGTGARDEDGRPLLGDV
                     VLAIESIQLEAHELAVPVAHHLQHLVVHGTLHLMGYDHETDADAEVMEGLEVAVLQGL
                     GVPNPYDD"
     gene            complement(19627..20682)
                     /locus_tag="PK264_04980"
     CDS             complement(19627..20682)
                     /locus_tag="PK264_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD75278.1"
                     /translation="MSRCLRDPDHGYYRTRDAIGRGGDFITAPEISQVFGELIGLWAA
                     AIWLEWGSPSRIAFVEIGPGRGAMMRDMLRAQRVVPGFLAAADIHLVEPSPHLRSVQQ
                     AAFAGLAAAPSWHADLAALDTATAAQAPERTPMLVVANEVLDTMCPEQFVRTTDGWAG
                     RGVGLDETGRLAFVTLPSRPGDGSPSSSVGAELDRIYANAVPGDIVEWADYDIAPLLA
                     RAGTTQLYIDYGRTAPRIGDTLQAVRNHRFEHPLTSPGEADLTTEVDFPALARRIARE
                     PHLKLHGPITQAEFLGRLGIIERASRLMAANPTDAPSIEAGVARLMAPLGMGGRFKAV
                     AIAPRDAPTPPGFAGQG"
     gene            complement(20811..>21270)
                     /locus_tag="PK264_04985"
     CDS             complement(20811..>21270)
                     /locus_tag="PK264_04985"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRD75279.1"
                     /translation="WMFSISDIVTAAVPIGLFFGRIANFINSEVVGTITSAPWGVPFP
                     PAEVWGSEPRHPAQLYEAGLEGLATFVILRFLTHRAGALASPGLVAGTFLVCYGAFRI
                     VCEFYKHDEHRALLGDLPMTSGMVYSVPMVAAGLAVITYSRRKSQLPALP"
     assembly_gap    21271..21398
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21399..>21651)
                     /locus_tag="PK264_04990"
     CDS             complement(<21399..>21651)
                     /locus_tag="PK264_04990"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRD75280.1"
                     /translation="LIKVMQALAIAFPAIDPVALRIGPVAVKWYGLAYVAGLLLGWLY
                     IRSLLARRALWGDRGPPIGVDRADDLFIWVALGVVVGGRL"
     assembly_gap    21652..21881
                     /estimated_length=230
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21882..22814)
                     /gene="recA"
                     /locus_tag="PK264_04995"
     CDS             complement(<21882..22814)
                     /gene="recA"
                     /locus_tag="PK264_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRD75281.1"
                     /translation="MATPHLMVVEGDRMDKHKALDAALAQIEKHFGKGSIMRLGTGDR
                     VMDVEAIPTGSLGLDIALGIGGLPKGRIIEIFGPESSGKTTLALSVVAEAQKRGGLCA
                     FVDAEHALDPVYARKLGVRTEDLLISQPDTGEQALEIADTLVRSGAMDVLVIDSVAAL
                     TPKAELEGEMGDSLPGMQARLMSQALRKLTGSISKSKCMVIFINQIRMKIGVMFGNPE
                     TTTGGNALKFYASVRLDIRRIGQIKDGDEPVGNQTRVKVVKNKVAPPFKQVEFDIMYG
                     EGISKSGELVDLGVKANIIEKSGAWFSFDGNRIGQ"
     gene            23022..>23420
                     /locus_tag="PK264_05000"
     CDS             23022..>23420
                     /locus_tag="PK264_05000"
                     /inference="COORDINATES: protein motif:HMM:NF021376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HRD75282.1"
                     /translation="MVKAAGFLPGLEIEFPHHPLPKHRRRPTSRPATTLEAPSKMTPD
                     ERQMIIGLFDRLRAAGPQDKDREAEALINQLVRQTPDGLYLLVQTALVQDFELQSAQQ
                     RIEELEQALAGRSAPASSGSFLGRGAAPASA"
BASE COUNT         3914 a         7300 c         7933 g         3872 t
ORIGIN      
        1 caaggagcgc aagggcgagg cacgccgttc tgacctgtcg tctcgtcatt ccgacctcgt
       61 cgcgtccgcc gctccgtctg gcccgggcga caccgttggc gaagcaccgc atagcacggc
      121 tgtcggccgt acacgaatgc atggaaattc atcgggactt gtcttgcggg cacgatcgtg
      181 tcgacataaa gtccgcaacg ttcgcgcgta cgaaggcacg gaaagtccgg gccggcgcgg
      241 gccgctcggg cgcctaggtc gcccccgacg tcgaggcaag gagccgacca tgttcaaggg
      301 gtcattccca gctctcatca cgccgttcaa ggacggagcc gtcgacgaga aagccttcgt
      361 ccgttttgtc gagtggcnnn nnnnnnnnnn nnnnnnnnnn nncccgtcgg caccaccggc
      421 gagagcccga cgctgacgca cgaggagcac aagcgcgtcg tcgaactcgc agtcaagacg
      481 gcgcgcaagg cgaagcgcta cgtcccggtg atcgccggct gcggctctaa ctcgacggcg
      541 gaagccatcg actacacgaa gcacgccaag aaggtcggtg cggatgccgc cctgcacgtg
      601 ctgccctact acaacaagcc gacgcaggaa gggcagtacc agcacatcaa ggcgatcagc
      661 gacgccgtcg acctgccgat cttcatctac aacatccccg ggcgtaccaa catcgagatg
      721 tcggtcgcta cgatggcacg ttgcttcaag gatctgaaga acgtcatcgg cgtgaaggat
      781 gcgacggcga agatcgatcg cgtgacgctg cagcgcctcg cctgcaagcg ccccggccag
      841 gagttcatcc agctctcggg cgaggatgcg acggcgctcg cggtgatcgc gcacggtggt
      901 cacggctgca tctcggtgac ggcgaacgtc gccccgcgcc tctgtgccga gttcgagaac
      961 gcctgcctct ctggcgactg gaaaacggca ctggcactcc aggatcgcct gatgccgctg
     1021 cacgatgcgc ttttttgcga gacgtcgcct ggtccggtga agtacgcagc tttccgcctc
     1081 ggcctctgct cggccgagat gcgtcttccc atggcgccga tcgcggagga gagccgcaag
     1141 cgcgtcgatg cggccctggt tgcggtcggt ttgtcgaacc tcgcaaaggc ggcggaataa
     1201 ctatcggctt cggccgatgg cgtccgatcg atagcatcac cctcggctcg cgatcgggtc
     1261 gggggtgagc ttttttcgcc cagacctttt tagcaagatc ttttggtcag gatcagtgat
     1321 ggccgccaag aaggacgaga accgcaagac ggtcgcggag aaccgcaagg cgcgcttcga
     1381 gtatcacatc gaggagacca ttgaggccgg catcgagctc aagggcaccg aggtcaagtc
     1441 gctgcgcctc gggcaggcca acatcgccga gagctatgcc tcctccgagg ccggaggcat
     1501 catgctgatc aactcctaca tccccgaata caagatggcc ggcgccttct tccagcacga
     1561 gccgcgccgg ccgcgtcgcc tgctgctgcg cgcccgcgag atccacaagc tgaccatcgc
     1621 catcgagcgg caaggcatga cgatggtgcc gctcgagctc tatttcaatc agcgcggctt
     1681 cgcgaagctc cgccttgccc tcgccaaggg caagaagacc gaggacaagc gcgccgacat
     1741 caagaagcgt gattggcagc gcgagaaggc ccgcctcctg cgcgacaagg gatagcttcc
     1801 agcgatccgc ctatgcactg ttctcgatga gcgcttttgc ggtggcaagc gtatccgcct
     1861 ccgccgcggg cttgtcccag cgaatgcgat gcacgcgggg aaagcgcatg gcgacacccg
     1921 acttgtgccg tgcggaccgc tggatcgcat cgaaggcaat ctcgaggacg agttccgccc
     1981 gtacggcgcg gacgggccca taggtctcga cggtattcgc ccggatgaag cgatcgaggc
     2041 gcacgagctc ctcgtcggtg aagcccgagt aggccttgcc gacgggcacc aagtcgggtc
     2101 cacgctcgcc ctcgcgccac acaccaaacg tgtagtcgga atagagcgac gagcgcttct
     2161 ccgagccgcg ctgcgcgtac atgagcacgc aatcgagcgt cagcgccgcg cgcttccact
     2221 tgtaccagtg gcctttcggc cggccggcga gataggggct gtcggcgcgc ttcagcatca
     2281 ggccctcgat gccggcgtcg cgggcgccgt ctctgaggcg tcgcagcgca tcgaggtcgg
     2341 cgaacggcac gatggtgctg agatcggtga ggcgcggagg cgcagcgctg tgccaggcct
     2401 cgagctcgcg gcggcgttcg agaagcgggc gcgggcgcag gtcacgctcg ccgatggtca
     2461 gcacgtcgta gagccggacg tgcgccggat ggtcggcgag ctgtcgccgt gtcacggcct
     2521 tgcggttgag acgctgctgg agctcgttga agggggcgac gatgccgccg cgcacgacga
     2581 gaagctcgcc gtcggcggtg acctcgcgtc cggcgaacgc ctcggcgatc tcgggaaagg
     2641 ccgatgatat gtcgtcgccc gtgcgcgaga agaggcgcgt ggccccgccg cccgccgcga
     2701 cttggacacg gatgccatcc catttccatt cagcgacgaa gtcgctcggc ttaatcgcgg
     2761 gcaagtcact gtcctcgagc ggatgggcga gcatcagcgg ccggaacacc ggcttgccga
     2821 gcacgtcggg gcgcggcgcg tgtccttcga gccaggcgaa gagctctcga tagggtggtg
     2881 cgagcgcgtg ccagacctcc tcgacctcgg cgacctcctt ggcgccgatc gctgccgcgg
     2941 cagtcttggc gagacgcgcc gagacgccga ctcgcggcgc gccgccgacg agcttgagga
     3001 aggcccagcg gccgcgtgaa tcgaggcgat cgatcgtccc ggcgatgaag cggccgagcg
     3061 acgaggacgg cgttgtggca agcgaggcaa tgagatcggc gagaatcgga gcgggagagc
     3121 gcgcgtcgcg gctgtcgggc tccggccaca gcaaggccac ggtctccgcc gtatcgccga
     3181 cgaagtcgcg cgaaagcttg tagagcaccg ggtccatgcg tgcggcgatg agatcggcga
     3241 ggatgcgccg gatcggcacg cggatcggca gtccgtcggt cagcgcggcg agcgcatagc
     3301 cgcgatccgg atcggggacg gcacggaaat attcctccat tgccgcaagc ttgccgttgc
     3361 gcgcgggagt gaaggcgagc gtgtcgagaa ggcgggcaaa gcgctccatg ggctattccg
     3421 cctcgtcctc gtaaccgacg agggcgagtg ctcgggcgct gcgacccatg ctcgcgagct
     3481 ggtggacgag cgcatcctcg cgaccgtgcg tcacccagac atcagacgct tccgtctcga
     3541 caatcgtcga gagtagctca ggccaatcgg catgatctga gacgacgagc ggcagctcga
     3601 cgccgcgctg gcgagcgcgg gcacggaccc gcatccagcc cgacgcgaat accgtgacgg
     3661 catcgccgaa gcgccgcgac cagcggtccg aaagcgagga gggcgggcag agcacgagcg
     3721 cgccctcgag ctcggccggt gtcgcggttg caacgggcct gacgtcgccg aaatcctgac
     3781 cgaagttttg gtaaagcttc gtcagctcga cgagcgcgcc gtgcaggtag atcggccggt
     3841 cgtagccggc gcttcggacg agcttcagca tgcgctggca tttgccgagg ccgtagacgc
     3901 cgagaaggtg cgtgcgccgc ggctcgcgcg cgagggatgc gacgagccgg gcggcctcgt
     3961 gttcggcctt ctcgtggcgg aacacgggaa gcgcgaaggt tgcctccgtg atgaacacgt
     4021 cgcacgtgac cggttcgaaa ggcgcgcagg tcggatcgga cgcgcgcttg tagtcgcccg
     4081 atgcgacggc gcgcgtgccc gcatgctcca tcaccacctg caccgagccg aggatgtgac
     4141 cggccggcgc gaggcgcacg tcgacggcgc cgagccgcag cgtctcgccg ggtgtgatca
     4201 cttgaaatgc accggccgaa ttttcgccga ggcgaacttt catgatctcg atcgtctcgc
     4261 gggtggcgag gacggcgccg tgacccgcgc gcgcatgatc gctgtggccg tgcgtgatca
     4321 ccgcgcggtc gacaggccgc gtcggatcga tatggaatcc gcccggctcg caatagagac
     4381 cactcggcgt cggatagagc caggtgctga cggctggcat gggaacgctc ccgttcatca
     4441 ccgttcagac catgcatagc ggctaggacg tccggctgtc attgacaagg ccgcgctcac
     4501 gctcgatcgt cgcgcccgcg ttgaatgcca agatcggcga ggatgccgtg actggcggga
     4561 gttcagtgtg cggagcgcga agcggggcgt cgagcggcga tgatcgtcgg cactgcagga
     4621 cacatcgacc acggcaagac ctcgctcgtc aaagcgctga cgggcgtcga tacagaccgg
     4681 ctcccggagg agaaggcacg cggcatctcg atcgagctcg gctttgccta caccgtgctt
     4741 gaggatggcc agagcctcgg cttcgtcgac gtgccggggc atgagcgctt cgtgcacacg
     4801 atgctggccg gcgcgaccag catcgatttt gtcgtcctgg tggttgcggc cgacgacggt
     4861 gtgatggcgc agacgcgcga acacctgagt attctcggca ttctcggtct cagcgacggt
     4921 gtcgtggcgc tgacgaaatg cgatctcgtc gatgaggctc gcttggcggc ggtcgaggcg
     4981 gagatcaggg tggcactcgc ggcgtcgagc ctgcgggacg cggacattct gccggtctcg
     5041 tccgtgacgg gggctggaat cgcaggcttg cgcacgcgtc tcgccgagga ggcgatgagc
     5101 cgccccgcgc ggcctggacg tggccagctt cgcattgctg tcgatcgcgc gttctcgatc
     5161 gcgggggcgg gtacgattgc cgccggcgcc atcacgggcg gtcgcatcac ggtcggcgac
     5221 atcgtcgtcg cgctgccgag cggtgccgag tgccgtgtgc gctcgctgca cgtcaacggc
     5281 cgcgcggcca cagttgcaac gtacggcgac cgatgtgcgt tgaacctcgt cggcgccgac
     5341 ctctcgcgcg gcgatgtcgc ccgcggggac tggcttgtgg cgccggacgc acgcatggcg
     5401 accgcgcggg tcgatgcacg tgtcgagctg ctcgcgagcg agaagcggcc gctcgcaacc
     5461 tgggtgccgg tgttcgtgca cgtcgggacg tcggccgctc cggccaggat tatcgtgcta
     5521 tcgagcgaaa agttgctgcc cggaggcaat gcgctcgttc agctcgtgct gccaaaggcg
     5581 ttgccgctgc gccatggcga tcgcgtcgtc ctccgcgata tcggcgccga gcgcaccatc
     5641 ggcggtggga tggtcgtcga tccgcagcca ccctcgcgca agcggcggac agacgggcgg
     5701 ctcgcgatgc tggcagcgct cgaggagatt gttgcggccg aggccctaag ccgtctcgcc
     5761 gaggttgagg ccggtctcgt cgatgtgcac gcgtttgcgg cgggacgcgg gctgacgccg
     5821 gcggagttgg tcacggccct tgccgaggcg gggctcgtcg cggtcggctg tgcgaccgag
     5881 gggcaggtcc ctcgctttgc gaccagcgag tcgaggctcg acgcgctcgc aatgtcgttc
     5941 gagacggcac tcggcgcgag ccatgcgcgc gagccggcgc tcgcggcaat tcctctgcaa
     6001 cgtgtgcgtc tcgcaatgtc gccgcgcatt gcgcccgacg agtgcgagga cctggccggt
     6061 catctggtcg cggcggaacg cgtcgcccgc ctcggcggtg gcgtccgtct gccgaaccac
     6121 aagtcggcgc tggcggacgc agatatgcgg gtctgggata agctcaggcc gcatctgctc
     6181 gcaaagccgc gacatccgcc gacgctgcga gaaatcggtc tagcgatcaa ggtcgaggtg
     6241 cagggattgc gccgcgtggc gaagcagctc gcgcgcctgg gcgagctcgt cgagatcggc
     6301 gccgatcgct actttgcgcg ctccgccgtg atcgagcttg ccgaggtcgt cgccgagctc
     6361 ggccgcacgc gcgcgctcgg cgtcttcacg gccgccgatt tccgcgatgc ggccggcaca
     6421 gggcgcaatg tggggatcgc cgtgctcgag tatttcgatc gcgtcggcat cacggtgcgc
     6481 cgaggcgacg agcgtcggcc cggtaagccg gccgccgagg tgctcgcccg gcggccttga
     6541 ggtgtccgat gtcgtcagga ctccggcgtc ttattgtagt cggcgtcgga gaccggttcg
     6601 agccaggtgg cgctctcgcc ggctgcatca cattcggtga gggcgaggtg gacgaaggaa
     6661 tgccccggcg ccgagccgtg ccaatgcttc acgtccttgc cgatcacagc cgtatcgccg
     6721 ggattgagca cttgcagcgg cttgcctgcc tcctgcaggc gaccgacacc cgagagcaca
     6781 tgcagcacct gcgcgacggg gtgcgtgtgc cagttggtgc ggccccccgg catgaacgag
     6841 accttgttga cgcgcagccg cgacggcgcg tctggcgccg gcaccagctc gtcctgccag
     6901 acgccgccga cgaaccattt ggcgggcgcc gtcttcgtgg ggcgtgcatt tggcctgtag
     6961 attttcatgt gacgtgacct ttccctgaag cgtctccgtc gcgagactag gagcgattgc
     7021 ccgatctgag aagcgcgtcg caggacgggc ggtcagcgct ccgcggggat cagttcggcg
     7081 accggctcgc cggcagcggc cctgcgggag aggccttcga attcggaggc ggcgatgggg
     7141 tagaactcca cctcgtcgcc ggcgcagacc aggaaggcgg gatcacggcc gtgcatgaag
     7201 ttgcgcacgg gcgtcgtgcc gagcaggtgc cagcccgagg gggcttcgac ggattggatg
     7261 ccggcctgga tgccgccgat actgatcgtg ccggctggcg tcttcagacg cgggctgtcg
     7321 cggcgcggga tcgcgatccg cggatcggcg ccgccgagat aggcgaagcc cggcaggaag
     7381 ccgatcatgt agcagcgata gacgggcgcg ctgtgccgct cgatgagttc ggctgccgac
     7441 atgccgaggc gggcggccgt cgcgtcgaga tcaatgccgt agtcgccgcc gtagacgacc
     7501 ggtacgcgcc agcggcgcgc tgcggcaacg agcggctcga tggaccgcgc catctccagc
     7561 acggcggtag cgagcgcatc gatgtcggca acgaaaggat cgacgtggac ggcaatcgag
     7621 cggtaggtcg gcacggtctc gacgatcccg ggtccgagcg tgcccgcctt cacgccggca
     7681 aggatggccg catcgagggc gagcaccttg ccgctgagca ccggatcgac cgtatcgccg
     7741 aactcgacgc cgacggcctg atcgccgaga gtcagaagcg ctgcggtgtc atggggcgcc
     7801 atggtcagct cttcgtcacg cggttcggga ggtacatgac gatctcggga aagatcgcga
     7861 ggagcaacgt gaaaccgacc atgatgaaga ggtagggcat ggtcacgcga gcaatgtagc
     7921 cgaggctgtc ctcggtaagg ccctggatga cgaaaaggtt gaagccgacc ggcggcgtta
     7981 tctgcgccat ttcgacgacc atgacgagga agatgccgaa ccagatcgga tcgaagccgg
     8041 cggccttcac gatcggcagc acgatcggca gcgtcatgac gatcatgctg aagccgtcga
     8101 ggaagcatcc gaggatgaga tagaatgcga cgagcgccgc gatcaacatg aacggcgaga
     8161 gcccgaggct cgcgacccag tcggcgatcg ccttcggaat gccgaggaag gcgatggagc
     8221 tcgcgaggat cgaggcgccg agcacgatca gcgcgatcat cgcgcaggtg gtgacggcgc
     8281 ccatcaggat gctcttggcg ttctcgaagg tgagtgttcc ttggatcgcc gtgacgatga
     8341 cggcgccgag cacgccgacg gcggccgact cggagggcgt tgcccagccc cggtacatgg
     8401 agccgagcac gcagacgatg aggaacacgg ccggtgccag gtctttgagg gcgcggaagc
     8461 ggtctgccca gggaatcgag ccgagcgcat gctcggatgc cggcacgagg ctcggcctca
     8521 gccgcgtgtg gatcatgatc caggccatga aggcgagggc cagcagaagt ccggggatga
     8581 tacccgccgt gaagagcttc aggatcgaga cctcgccgag cacgccgtag atgatgagga
     8641 tgttcgaggg cgggatgagg aagccgagcg tgcccgctcc ggcgagcgag ccgatggcga
     8701 tgtcccgact gtagccgcgc ttcaggagct cggcgaggga gagccgtccg accacgagag
     8761 tcgttgccgc ggacgatccc gacatcgccg cgaagatgga gcagccgacg acgttgacgt
     8821 gcaggagccg gcccggcagc aggccggccc agggagcaag tccattgaag agcgaggccg
     8881 agagccgtgt gcggaacagg atctcgccca tgacgatgaa gagggggagt gccagcagct
     8941 cctgggtcgt caggatgttc caggtgtact gcgcgagcag cttgtcgagc ggcacggact
     9001 tgaagaggcc gagcatgacg acgcctgtga gcgcgagcgt cagcccgatc cagaggccgg
     9061 cagagagcgc gccgaagagg accgcaaaca gcgtgagcac ggcggtcagc atggtcagcg
     9121 atcctcaggc agtgatagcg acacgacggc ctcgaggatt gcgctcacgc gagcacgaca
     9181 ctggccttgc gtagtgcgat ttgggccggc agccacgggc acgtcaagga acattggttg
     9241 gtgcgtacac ggagtgaaac ggtgttgacg acgacgggga cacactgttg gcacagcgga
     9301 tggcctggtt atcatcgtcg aaagcgatca tgttgcaacc gggttgatcc ggcacgtggc
     9361 agcacgatcg accactggag cactgggcat ggcagagccg gacggtctcg atgggtcagc
     9421 actcggccgg cgcggctcga gtcggagccg tatgagcgcc ctccgcaggc gcgtctacga
     9481 gatcatagag cttgcgcgcg aaggtgatgg cgtcagccgg ctcgtggatc tcgggctggt
     9541 gattctgatc ctgctgaatg tcgcagcgtt cgtgctcgag accgtccccg agttcgaggt
     9601 ggcctaccgg agccagctct actggttcga actcttctcg gtcgcggtgt tcacgatcga
     9661 gtacacggcg cgcatctggt cgtgcgtgga aatgccgttc ctcaatatga gggcgccctg
     9721 gctctcgcgc ctcaagtacg caaccaagcc gtatctcctc atcgatctgt tggcgatatt
     9781 gccgttctac ttgagcttcg tcgtaccaat tgatctgcgc gtgctgcgcg tgttccgact
     9841 gttccgcatc ctgaagctcg cccgctattc gccgacgatg cacatccttc tgagggtgct
     9901 ggcgcacgag cggcgcacgc tggtcggtac cttcctcctc gccatgacgc tgctgctgtt
     9961 cgtcgcgacg tcggcctact acgcggagcg gatcgcgcag cctgagaagt tcggcacgat
    10021 ccccgatgca gcttggtggg cgatcgcgac gctgacgacg gtcggatatg gagacgtggc
    10081 gccgatcacg ccactgggca agatcatcgg aggcatggcg atgatctgcg gcatcatcgt
    10141 gctcgcgctg cccattgccg tcatcgcgac aggtttctcg aacgagattg gcaagcggga
    10201 cttcgtcgtg tcctggccgg tgatctcgcg cgtcccgtcg ctcgcctgcc tgcgcgacga
    10261 tgagatcgac gcggtgacgc catttctcgc ggcacagttc atggacccat atcgtgaggt
    10321 cattgccccg gaggatcgtg cggacgccat gttctttgtg gtgaggggga gtgtccggtt
    10381 gcgtaccaac gccggagaga cggtcttccg ggtgggagac ttcttcggcg aactcgccat
    10441 gctcgaaggg cgaagccaca cccattccta ccgcacgacg gaaccgacgc gattgctgcg
    10501 gctcgccaag gacgatttcg agttcctgtc cgagagccat ccggcgattg cgcatcagat
    10561 catgtcggtc gcccggaagc ggcggacggc ccgcgaggcc ggcgaagccg accccgcagc
    10621 gggcacgtcc acgccggcgg cagatcatag cgcctgaatc aacaaggcgc ctcgcgccgg
    10681 tggcgcggcg ccccgcccga cgtcacttca cgacgatagt gatgttgtcg ctggtcatcc
    10741 gcacgccctg aatgcacttc ccgcctttgg catcgtgcag cgagaagacg tagcgcatgc
    10801 gcgtcgtgac ctcgcgcttc gatttggcct tgacggcgcc gagcgaaatg tcggcgccgt
    10861 tgttgcggat caccttgacc tcgatgtcgg tcgcccgctc gttctcgatt gtgagctttg
    10921 cggcggggcc gatcttggga gagcggtgct cctcgagtga ggcgcagtcg atcttgaagt
    10981 cggttgcaag caccggcgcc gcggtgatgg cgcccgatac ggcgacaagt gccgatgtga
    11041 tggcgatagc ggtcggtctc atccagcctc tcctccaatt gaaagcccga tcgaagaact
    11101 atgcctcagc cgatgagcga gtgccagcgc tgacacgtgg atcggcgcta cgccgaggct
    11161 cgacggcccg cacacggcaa aggccccagc cgggtcgcgg cgtggggcct tcggtggcgc
    11221 agtgattgag aggccgcgag cgagcggcag atgatcagac ggcttccttg agctccttgg
    11281 cggcgcggaa ggcgaccttc ttgctcgcct tgatcttgat ctgctcgccg gtccgcgggt
    11341 tgcggccgag gcgggcgggg cgcttcttga cctggaggat gccgaggcca cccatgcgaa
    11401 tgcgggcgcc ctttttaagg tgtgtgcgca ccatcgccac catgtcctcg agcagcgcat
    11461 aggcctgctt ctttggaatg gagtgggcat cggcgagggc agtgccgagg tgacgcagcg
    11521 tgacagtggt cgtcttcgcc tttgcagggg cggctttcgc gggcgcggct ttggcagcgg
    11581 ctttggccat gtgtgaattc cctttgagtg gtgtgggggt ggttggtcgg gcggatactc
    11641 gtatgagcgg atcggccgaa cagtcggcaa cgcatgaaaa taaatatgtc agcgaggcac
    11701 caaaccatag ggaaatatag atattccgcg atcattcagc ggcgcgacgc gcatttcgtt
    11761 gttgttttgc aacggtttct gcgcagagca tccggaaaac atcggctatt cagcgagttc
    11821 tgctgccact tcgcgaacgg ccgccgctct ggcgggcgat cctgggcaca ggacagcacg
    11881 agtgattcgc tcgcgcggcg ctcgacgggc ggcgtcagga acgtgctggc gcgtttgcgg
    11941 gattgtcgcg ttcgccgtct gcgtgccatc gtctggtcca ccgatccgaa tgcctggagc
    12001 cgcagccatg acgagcacga tcattacagg actgtcgacg acgctgtttc gcgtgccgtg
    12061 gtccggcgag ccgcccgcga acggcatcat gccgccggcg gcgcgcgaac tcctcgcgct
    12121 cgaggtgcgc accaagggcg ggctcacagg gctcggctat ttgcagccat tgagcggtgg
    12181 catcgagacg ctcgaccagt gcctcgccga gatgatcgcg ccgaagatca tcgggcgcga
    12241 cgcgaccgag gtcgagggga tatggcaggc gctctggaaa tccacctact ggctcgggcg
    12301 cggcggcatc gcgacgtttg cgcagtcggc ggtcgacatc gcgctctggg acatcgtcgg
    12361 caagaaggcg gggctgccgc tgcatcgcct ttggggatcg atgcggaccg aggtcccggc
    12421 ttacgggtcg ggctgctggc gcgggctcgg ccgcgacggc atgatcgcac gcgcacgcca
    12481 tttcacctcg aagggattca aggccatcaa gatgcaggcg gcccacatcc ggccgtggcg
    12541 cgaagacgtg aggaacgtgc acgccatgcg cgatgcgctc gggcccgatg tcgagataat
    12601 gatcgatgtg aacatgggct gggacgccgc gaccgcgatc caggccggcc gtcatttcga
    12661 cgaggcggac atctattggc tcgaggagcc ggtggtctgt gaggacttcg acggatacca
    12721 gcgcgtcgcc gcggccttga agacgcgtgt ggtcggcggt gagagccatt tcacgcggtc
    12781 cgacttgcgt ccgtttttca ggaccccgac cttgccgatc ctgcagccgg acccgatgcg
    12841 gggcggtctt acggagctgc gcaaaatcgc tgccgtcgcc gatacatggg gcctgcagat
    12901 cgcgccgcac ctcttccacg agctgaccgt gcatgtcctc gcctcgatcc cgaacgccaa
    12961 ctatctcgag tacatggact ggaacgacga cctgtgggtg acgccggcca ttccaaagga
    13021 tggcatgctg cgcgcgcccg aagcgccggg ccacggcctc gccttcaagc ccgagatctt
    13081 gaagagctgc cggttcggcg ggcgggaaac gtcgcgcggc tgagccgagg cgcgctcaac
    13141 cgccgacctc gtcggcgatg cgggcgacga cgccctcgac ggcggccggg cggcgaatga
    13201 tgttggtcag cgctgagaag attgtgtcgc cggcgagcca gcgcgcctcc gcgtccggcg
    13261 tggtgagcgc cacctcgagg gctgccgcaa gctcgtagcc gatcgtcggc atgccgacgc
    13321 tcttcattgc agcgagcagt gcgaggtgcg cgttcgaccc ggcggccatg tgccggttcg
    13381 gcacggtcat caggctgcgg tcgccctcga gctcgctctt gcaagcctcg accaagaacg
    13441 aggccgtttg ccggcagacc agcgggcgga cggcgtagac gctgcacgcc cccgcggttc
    13501 cgagcagctg gcaggcgatc ttggcgccga tccgttcttc gggagatttg tcacgcgtcg
    13561 cccgaccgcg ctccaggact gccgccggct cgaggaacgt gttgccctgc ctcgccccct
    13621 ggcggatggc gcgggcgatc gcgaagacct cgggcgggat gacgccgaca tagttgtggc
    13681 agcagtgggc gcagcccttc gcgcaggcaa cggcttcctt ggcggcgaag gaggcgcgcg
    13741 acgcctcggc gagcttctgg gcgaggcccg ccgcttcgct cgcgcgcgta acgttgcctc
    13801 gttccttgag cttggccctc agaacgaggg ctgccccata gacctcctcg cggcgcggct
    13861 ccggccgaag gccgtcgtcg aatgccgccc ggccgatctt cagccagtcc cgctggcggg
    13921 cacgtcgttg ctgtcggctt tcgtcggcca tgacagatgt cgatccgtgc tcgtctcagc
    13981 tgcagctacc gccgccgagc acgcagaact tggcacaatg cacgtggcaa agctttacag
    14041 cgcccttgtc gaacatgccg ccgtccgccg cgccggcctc ggtgagcgtg gggcccatgt
    14101 cgaattgggg ctgataaggg atgagcgtgc aggggagcac ggtcggccga tccgcgccct
    14161 tgcgcttcac gatcatgcgg ctcgtcgcgc acatcacatc gacagggcgc ttgttcagga
    14221 tgccccagca tgcattggtg atctcaggca cctcggcctt ctcgttcatc tcgggcagga
    14281 gatggagtgc cgcatggtcg ttcgcgtcga tctgccagcg ccgcgccgcg atcagctcgg
    14341 catagcccgc acgtgcggcc ggctcgctct cgccccagca cgtgcggccg gcgatggcga
    14401 gcgtgaagcc gttcttcgag agccagtcga tgccggcgag cgccttctcc cacgacttcg
    14461 gcccgcgctc gcgctcgtgc agctccctcg tgaagtgatc gaggctgatg cgcagcttga
    14521 gctggcggcc ataggccgaa ttgagccgca gcagctcctt cttgatgcgc ggccgctgca
    14581 tcggctgcat ggcattgctg agcacgagga cctcgaagcc ccgttccaag ccttcctcta
    14641 tgatcgcgat gatgtcgggg ttcatgaacg gctcgccgcc ggtcaggccg atctccttcg
    14701 tcccgagccg cttcgcgagg atctcgtcgt aaaatgcgac gacctcggcg gccgacagat
    14761 agacgagccg gtcgttcttc gggctcgatt cgatgtagca gctcgcgcaa gtgatgttgc
    14821 agagcgtgcc ggtattgaac cagagcgtct gaagccgcgc gaaggggacg gtcgcccgcc
    14881 gctcgccctt ggccgtccaa tccggatcct cgaacttttg cagcggacgg gtggccccgc
    14941 tgagtaccct gccatcagcg ttcaacttct gcctcgtttg ctcgtgcgtc agcggagctc
    15001 ctttggctcc ggctcgaaga gttcgtcgtg atccgacgaa acactagcac gatgatcgag
    15061 gcgcggctaa gtcacgttcg cttgtccgtc cgtgagcggt tgccggggca gacgaggcga
    15121 gctcagctgc cgctgcgggc cgtctgcggc cgccacggcg tgctcatgtc ggtcggcacg
    15181 agcgtgccgc gcggattggg atcgagagca cgggggcgcg gcgccagcgg cgcgggcggt
    15241 gccttcgtgt cgccttgaag acgtactcgc tcgcgagatc gctcgtgagc cgcgcgccga
    15301 actcgagctt ctgcttgaag gacacccaga tctgcccggc ctgggcgcag gtctcgccgt
    15361 tgcgatcgca gaaggtcgcg gcacgataag cggcatccca ggctgcagcc ttgagctgcg
    15421 cctgcttctt ctcgtcggtc ggcagcagca tgacgaccac tgcaagccag aacgcggttc
    15481 ggataaggaa catgacagtc gcccctgagt ggcgggttcc tgtccgttat gacacggagg
    15541 tcgcaagaca tcgcaaagtg gcacgctggc aattgccgct agtttcctcg gtatttcgaa
    15601 gtgaattgcc gcaaattgcg cggatcagtc cttgaccgcc tcgccctcga tctcgacgag
    15661 gtactcaggc gtcgccaggc ccgcgacctg gagatatgtc gctgtcgcct tgtggccgag
    15721 cagcgcctcg tcacgaagcc tgcggaagag cccgacctgg cccgggagcg tgcagaacac
    15781 ggttaccttg actacatgat gcggctcgaa gcccgccgcc ttaacgaccg cagtgaagtt
    15841 ggaccaggcg cgccgcatct gcgcctcgat gccgctctcg atcttaccat cggctgcgac
    15901 gcctacctgc cccgagataa cgaggcgcgg accagctcca tgcacgatgc cctgcgaata
    15961 gctcgacgcc ggcttcgcaa ttgtctccgg gttgatcatg cgcatggcga ggctcctcgg
    16021 gttccggggc ggcgccgtgc tcagccccac aggtaggcga agaatgcaat ggcaaagcca
    16081 gacaacgcaa tgatccaagc gaggatggca agccagcggc cgacgacgtg cttgccgatg
    16141 tcgacggtgg ccatcctggc gcgctcggcg ttcgagggtt cgtcgctgta ggcctggatc
    16201 cgcttctgca gctcggtgcc ctcgggcatg ccgcgtttgc gggcagcggc ccaggcggcg
    16261 tcggtcgcca tcgacttgag atcgtggcgc tgcgcccagc ggcgcaaaat ataggcgact
    16321 acgatgaggc cggctgcaac ggcgaaccag gcgagcttac cggtgatcat gtgcgacctc
    16381 gcgggctgtg ggactcgatc aggtatcgga tcaagagcgc acagggaagg gacggctgcg
    16441 gacnnnnnnn nnnnnnnnnn ncactttcgt cgtggagagc cgctccggct ggacgttcca
    16501 cggtccgcgg gctattttgc gctgcagcaa tagcgatggc gtcaactcct cgtcaaccac
    16561 ggttcctaac cggaaacgaa ctcgggccgg atagcatggt cggcaaaaag agtgtgtcga
    16621 acggagagca ggccatgccg gggggcaaga aggtcttcat caagacctat ggctgtcaga
    16681 tgaacgtcta tgacagcgag cgcatgtctg aggcgctggc gcctgccggc tatgccgaga
    16741 cgtccgagat ggccgatgcg gacctgattg tcctcaatac ctgccacatc cgcgagaagg
    16801 cggccgagaa ggtctattcg gagcttggcc gggttcgcga cgtgaagtcg gcgcgggaag
    16861 cccgcgggcg gtcgacgatc gtcaccgtcg cgggttgcgt cgcacaggcc gaaggcgccg
    16921 agatcatcgc ccgggcgccg gtcgttgatc tcgtcgtcgg gccgcagacc taccatcgcc
    16981 tgcctgattt gatcggccgc gccggttcga gcagcggcgg gctcgtcgag acgtcgttcc
    17041 cggatgagga caagttcgag catctcccga gccgcgaagg cgcgcggcgg cgcagtgccg
    17101 cggcgttcct cacggtgcag gagggctgcg acaagttctg cacgttctgt gtcgtcccat
    17161 atacacgcgg catggagacg tcgcgcccgg tcgagcggat cgaagccgag gcgcgccggc
    17221 tcgtcgagca gggcgtgcgc gagatcacgc tgctcggcca gaacgtgaac gccttccatg
    17281 gacccggccc cgacggcagc gcctggtcgc tcgcgcagct gcttcgccgc ctcgccgaga
    17341 tcgagggcct cgagcggctg cgctacacga cgagccatcc gcgcgacatg tcggccgacc
    17401 tcatcgcggc gcatgccagt gtcgccaagc tgatgccgta tttacacctg ccgttccagg
    17461 ccggttccga ccggatcctg gcggccatga acagaaagca cacggcgcag gactatcgcg
    17521 agctcgtgtg tcgcattcgt gcggcccgtc ccgacatcgc gctctcgacc gacatcatcg
    17581 tcggcttccc gagcgagacc gaggacgatt ttcgcgccac gctcaccatg gtcgaggaga
    17641 tcggcttcgc gcaggcctat tccttcaagt acagtgcgcg ccccggcacg cccgcctcga
    17701 ccatcgagga gcaagtgccg gagggcgtga aggcatttcg cctgctgcgg ttgcaggagt
    17761 tgctcgaaag ccagcagcgc cgcttcaatg cgggctgcgt cggaaagacc ttccccgtgc
    17821 tcttcgagcg tcccggacgc cactccggcc agctggtcgg ccgctcgccc tacctccagc
    17881 ctgtccacgc agacgccgag cccgggttgc tcggccgcat catgcccgtc gaaattccgg
    17941 cggcagggcc gaacagcctt tctggcgtca taaaggcggc ctgacagtga tgtcgagggt
    18001 ggcaggtggc ggaagcggtc cgcgccacgc ccgaagcgga ggctcgactt gacaggaacg
    18061 cgcgcgcccc ggtcggcagg tgcaaagccc gcgttgcggg cacagggagg cagcggcaaa
    18121 gccgcgaggc cggagctggc gaaaatcgtc gttcctttcg aggacaaccg gctcgtcgcc
    18181 gagctgctcg gcgagcacga cgctcatctg gcgctcatcg aggagcggct tggcatcgat
    18241 ggacacgcgc acggcaacat tgtcacgctc gccggtccgc ctgacgccat cgagattgcg
    18301 cgtttcgcgc tcgagacgct ttacggacgc ttgcggcgtg gtgagcgcat tgccgcgggc
    18361 gatctggacg ggctcatccg gcacggtgag agcgcggtcg ggcccgccgt tgccggaggt
    18421 gcgcgggatc tcgccggcat ggcgcagatc cgcacgcgcc gccgcatcgt cacggctcgg
    18481 acgccgatgc agagccgata tatcgaggcg ctggagcggc acgatctcgt gttcggcatc
    18541 ggcccggcgg gcaccggcaa gacctatctc gcagtagcct tcgcggcggc gagcctcgaa
    18601 cgcggcgagg tcgagcggat catcctctcg cgccccgcag tcgaggccgg tgagcgtctc
    18661 ggcttcctgc ccggcgacat gcgcgagaag gtcgatccgt acctgcgccc gctctacgat
    18721 gcgctttacg acgtgctgcc gcccgaaaag gtggagcggc acctcgaaac gggcgtgatc
    18781 gagatcgccc cgctcgcctt catgcgcggg cgcacgctcg ccaacgcggt cgtgatcctc
    18841 gatgaggcgc agaacacgac caccatgcag atgaagatgt ttctcacgcg gctcggcgag
    18901 aacgccaaga tggtcgtcac cggcgatccg acccagatcg acctgccgtc ggggcaggtg
    18961 tcgggattga tcgaggcggt ccgcttgctc gaggggatca agggcatcgc cgcgatccac
    19021 ttccaggcgg cagacgttgt gcgcagtgac ctcgtcgcgc ggatcgtcgc cgcctacgag
    19081 gggcggaagt gacgaggccg gacgggcagc ggccaggcgc ggcgctcgcc atcgaaatcg
    19141 tcgaggaaag tggcaactgg gcggttgtgc ctgaggcagg cgcgctgatc gcgcaggcga
    19201 cagacgcgct cgcccgccat cccgcgatcg caggtcgcct gcctgcagcc gcgacgatcg
    19261 tcctcgcgtc cgacgccgat gtgcaggtgt tgaaccggac ctggcgcggc aaggacaagc
    19321 cgaccaatgt cttgtcattt ccggcgggaa cgggagcgcg cgacgaggat ggcaggccgc
    19381 tgctcggaga tgtcgtgctg gccatcgagt cgatacaatt ggaggcgcac gagctcgccg
    19441 tgcctgttgc ccaccatctc cagcatcttg tcgtccacgg caccctgcat ctcatgggct
    19501 atgatcacga gaccgacgct gatgccgagg tcatggaggg cctcgaggtc gctgtcctgc
    19561 aggggctcgg cgtgcctaac ccctacgacg actgagcctg gcccacgggg tgtcgagtcg
    19621 ggtccgtcag ccctgcccgg caaaacccgg tggcgtcggt gcatcgcgcg gggcgatcgc
    19681 gaccgccttg aaacggccgc ccatgccgag cggagccatg aggcgcgcga cgccggcttc
    19741 gatcgatggc gcgtctgtgg gattggcggc catcaggcgc gacgctcgtt cgatgatgcc
    19801 gagccggccg agaaactcgg cctgcgtgat cggcccatgc agcttgaggt gcggctcgcg
    19861 cgcaatccgg cgcgccaagg ccgggaaatc gacctcggtg gtgaggtccg cttcgccagg
    19921 agaagtcagg ggatgctcga agcgatggtt gcgcaccgct tgcaatgtgt cgccgatgcg
    19981 aggtgccgtc cggccatagt cgatgtagag ctgcgtcgtt ccggcgcgcg cgagcagggg
    20041 cgcgatatcg tagtccgccc actcgacgat gtcgcccgga acggcattgg catagatgcg
    20101 atcgagctcc gcaccgacgg atgacgacgg ggacccgtcg ccgggacggg atggcagcgt
    20161 gacgaacgcg agccggcctg tctcgtcgag gccgaccccg cgcccggccc agccgtcggt
    20221 cgtgcgcacg aactgctctg ggcacatggt atcgagcact tcgttggcaa cgacgagcat
    20281 cggcgtgcgt tccggggcct gcgccgcggt tgcggtatcg agggcagcca gatccgcgtg
    20341 ccacgaaggg gcggcggcca gaccggcgaa tgcagcctgc tgcaccgatc tcagatgcgg
    20401 gctcggctcc acgaggtgaa tgtctgctgc tgcgaggaat cccgggacca cgcgttgggc
    20461 gcgcagcatg tcccgcatca tggcgccgcg ccccggcccg atctcgacga aggcgatccg
    20521 ggacgggctc ccccactcga gccagatcgc tgccgcccaa agaccgatga gctcgccgaa
    20581 cacctggctg atctcggggg cggtgatgaa atcgccaccg cgtcctatgg cgtcacgggt
    20641 ccggtagtag ccgtgatcgg ggtcgcggag gcagcgcgac acgaagcggt tgagaggaat
    20701 cgggcccgtt tcagcaagct cggcctcgat ccgtatcgca agcggggtgg cgcgacgcag
    20761 acaggcgtcg tagggccacg tttcggatga cgggctttcg cgacctgccg tcacggaagt
    20821 gccggcaact gcgatttgcg acgggagtac gtgatgactg cgaggccggc agcgaccatc
    20881 ggcaccgaat agaccattcc agatgtcatg gggaggtcgc cgagcagagc gcgatgctcg
    20941 tcgtgcttgt agaactcgca gacgatccgg aaggcgccgt agcaaacgag gaaggtgccg
    21001 gcaacgagcc cgggagacgc aagcgcgccg gcccggtggg taaggaagcg caggatgacg
    21061 aacgtcgcga gcccttcgag gccggcctcg taaagctgtg ccgggtgacg cggctcgctg
    21121 ccccagacct cggccggcgg gaatggtacg ccccagggcg cgcttgttat ggtgcccacc
    21181 acctcgctgt tgatgaaatt cgcgatcctg ccgaagaaga gcccgatcgg cactgccgcc
    21241 gtcacgatgt cgctgatcga gaacatccag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gccggccgcc gacgacaacg
    21421 ccgagcgcga cccagatgaa gaggtcatca gcccgatcga cgccgatcgg cggcccccgg
    21481 tctccccaga gcgcacgccg cgcgaggaga gaccggatgt agagccagcc gagcagaagc
    21541 ccggcgacat aggcaagccc ataccacttg accgcgaccg gtccgatacg cagcgcgacc
    21601 gggtcgatgg cgggaaaggc aatggcgagg gcctgcatga ccttgatgag cnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctggccgat gcggttgccg
    21901 tcgaaagaga accaggcgcc ggacttctcg atgatgttgg ccttgacccc gagatcgacg
    21961 agctcgcccg acttcgaaat gccctcgccg tacatgatgt cgaactcgac ctgcttgaag
    22021 ggtggcgcga ccttgttctt gacgaccttc acgcgagtct ggttgccgac cggctcgtcg
    22081 ccgtccttga tctggccgat gcggcggatg tcgaggcgca cggatgcata aaacttcaag
    22141 gcgttgccgc ccgtcgtcgt ctcgggattg ccgaacatga cgccgatctt catgcggatc
    22201 tggttgatga agatcaccat gcatttcgac ttggaaatcg acccggtgag cttgcgcaag
    22261 gcctggctca tgaggcgcgc ctgcatcccg ggcagactgt cgcccatctc gccttcgagt
    22321 tcggccttgg gggtgagggc tgccaccgaa tcgatgacga gcacgtccat ggcgcccgag
    22381 cggaccagtg tatcggcgat ttcgagggcc tgctcacccg tatccggctg cgagatcaga
    22441 aggtcctcgg tgcgcacgcc aagcttgcgc gcatagaccg gatcgagtgc gtgctcggcg
    22501 tcgacgaacg cgcagaggcc gcctctcttc tgcgcctcgg caacgacact cagggcaagt
    22561 gtggtcttgc cggacgattc cggcccgaaa atctcgatga tgcgaccctt cggcaagccg
    22621 ccgatgccga gtgcgatgtc gagcccgagc gagcccgtcg ggattgcttc gacgtccatg
    22681 acgcggtcgc ccgttccgag ccgcatgatg gagcctttgc cgaagtgctt ctcgatctga
    22741 gcgagtgcag catcgagcgc cttgtgcttg tccatcctgt ccccctcgac caccatcaga
    22801 tgcggcgtcg ccatggttgc gccccttatt ccacgagatg ccaacggttt caaactgaac
    22861 tcagagtaca cgaattgttc tcatcgcgca actggatgtt cagcttttgt ttgcatcgga
    22921 gaacggcggg cactcgattg gcatttcgga tgtttcccga tcggtgtgga tggtgggtca
    22981 gctgtctggt aaggacaagc tccgggcctc gggtgaaatg catggtcaaa gccgccggat
    23041 ttctgccggg cctggagatt gaattcccgc atcacccctt gccaaagcat cgccgccgcc
    23101 caacatctcg gccggcaaca accttagagg ctccgtccaa aatgacccct gatgaacgtc
    23161 agatgatcat cggccttttc gaccgtctgc gtgctgctgg gccgcaggac aaggatcggg
    23221 aggcggaggc cctcatcaat cagctcgtgc ggcagacgcc cgatgggctc tatctgctgg
    23281 tgcagaccgc gctcgtgcag gacttcgagc tgcagtcggc gcagcagcgc atcgaggaac
    23341 tcgagcaggc gctcgccggg cgtagtgcgc cggcgagctc cggtagcttc ctcggccgtg
    23401 gtgccgcgcc tgcctctgcc
//
LOCUS       DAORNH010000029        23415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1673_length_23415_cov_2.747945, whole genome shotgun sequence.
ACCESSION   DAORNH010000029 DAORNH010000000
VERSION     DAORNH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23415
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_23415_cov_2.747945"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="PK264_05005"
     CDS             complement(<1..1419)
                     /locus_tag="PK264_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75283.1"
                     /translation="MSTAKSRYHEVYAAWKSDPDAFWAEAARDIDWYNLWDKVLDPYA
                     GEYGRWFAGAECNTAYNCLDRHVLRGRGQQKALIYDSPVSKTTRSYTYAELTHEVATL
                     GGVLLDLGIKKGDRVIVYMPMIAEAAIAMLACARIGAIHSVVFGGFAAAELATRIDDS
                     KPVAILAASCGIETQRVIPYKPLLDKAIELSAHKPKHCIVLQRPMAEAAMTAGRDHDW
                     EGLVKDAKARGRKAGCVPVAATDPLYILYTSGTTGAPKGVVRDNGGHMVALKWSMRNH
                     YGIEPGEVFWAASDVGWVVGHSYIVYAPLLHGATTIVYEGKPVGTPDAGAFWRVIAEH
                     KVAAMFTAPTAFRAIKKEDPQGELIKSYDLRAFRTLFLAGERADPETIKWAEAKLSVP
                     VIDHWWQTETGWAIAGNPVGLGQLPVKYGSPTVPMPGYDLQVLDPTGKPVKAGETGTL
                     AIKLPLPPSCLPTLWNNDERFRK"
     gene            1700..2128
                     /locus_tag="PK264_05010"
     CDS             1700..2128
                     /locus_tag="PK264_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HRD75284.1"
                     /translation="MSRLSLLSSPLLLGFEEIERLIERAAKAGDGYPPYNIERMTRSE
                     SAPETLRITLAVAGFKRDEIEITLEDNQLIVRGRQDDDKSREYLHRGIAARQFQRTFV
                     LAEGIEVRSADLAHGLLSIDLVRHEPERMIKRIEIRGAKP"
     gene            2321..2551
                     /locus_tag="PK264_05015"
     CDS             2321..2551
                     /locus_tag="PK264_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HRD75285.1"
                     /translation="MSRSELANLGDGHVAYIRVMSVAEARRRFPAVGTLPDVKDLYAL
                     HSADGTPIALTDSHEAAIGHAMGDDLVIASVH"
     gene            2723..3853
                     /gene="zapE"
                     /locus_tag="PK264_05020"
     CDS             2723..3853
                     /gene="zapE"
                     /locus_tag="PK264_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HRD75286.1"
                     /translation="MSRPENSRPEARYAERVARGEIEHDVAQAAIVAQLGALGDALAT
                     WRPQKRWSLSQMFGAKARPPRGLYIHGAVGRGKTMLMDLFFDAVAIEPRRRIHFHEFM
                     AEVHDRIATARKTVEGDPIPHVAREIAEGASLLCFDELHVTDIADAMILGRLFAGLFV
                     ADVVVVATSNSSPGELYRNGLNRQLFLPFIAMIEERLDVAQLDAAKDFRLEKLAGLPL
                     YFSPADADARRALSKAFQRLTGVPHGAPLTLDVKGRAVIVPEAASGVARFTFDELCDT
                     PLGSLDYLHIAHAFHTVIIDSVPLLVPERRAAARRFINLIDTLYDNCVGLIVSAAAEP
                     HELHTEGKEAQLFERTASRLVEMRSEAYLGERARRHDDLAAE"
     gene            4398..4835
                     /locus_tag="PK264_05025"
     CDS             4398..4835
                     /locus_tag="PK264_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRD75287.1"
                     /translation="MRTLFAALAAFGLVSAAAFAQGPATHKVAIHVNENNPATMNLAL
                     NNVLNIISYYKKKGETVAVEVVTYGPGLHMFRADTSPVKQRVAQIALENPTVTFAACG
                     NTAENMSKAENKPVSLISEARVVPSGVIRLIELQKVGYAYIKP"
     gene            4936..5940
                     /locus_tag="PK264_05030"
     CDS             4936..5940
                     /locus_tag="PK264_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD75288.1"
                     /translation="MRSAFRPIYGLLALSLLALAGSAIAQSPTEVRRDAVTKEKINSW
                     TIGLAAGRIEGTPLRLAAELAQALDNEDAMRVLPIVSRGPFDNMYDLLFLRGVDMAIV
                     YADNLDHFRTKERVPGLLDRVNYIARLFPAEVHLVVRPEINSLKDLVGKTINVNTAGT
                     AAAYSGPIIFQRLGLNVRLTNNPHRDALQEIRKSDKYAGLLFVTTKPVPPLAAPNWPE
                     GFKLLPIEMTPALEEYYVPAYLEAAEYPKLIPAGQRVTTIAVPVVLAVYNWNVGKDRY
                     RRMVRFVDYLFDRLPALQKEPNHPAWKQVNLAATVPGWKRYPAVQSKLDALKGGAPKR
                     "
     gene            6160..7149
                     /locus_tag="PK264_05035"
     CDS             6160..7149
                     /locus_tag="PK264_05035"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRD75289.1"
                     /translation="MRRHMLQLGAALGLAALSLTSSVALAQDRLAVFKESLRSKANAH
                     TVGVASGNPNGAYLYLAYDLSAVFDSEALRVIAVVGKGGQQNVKDALYLRGIDLAITQ
                     ANVLKHFKRTGELGSDIDKRLVYITRLYTEEMHVVVHQKSGVASLQELAGKTVNFSDA
                     GSGTQLSARVLFQLLGLNVKEINVGQADALRMLAAGEIAATVLVAGKPAGAFGRLAAD
                     SGYRILPIPYTQAMEDADFLPIRLSRTDYAIIPDGQPVDSLGVSAVLAAYNWPKSSDR
                     YARIERFINELFTRLAELQKPPRHPKWKDVNLSATLPGWTRHPAAQSWLDRQR"
     assembly_gap    7180..7189
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7220..7909)
                     /locus_tag="PK264_05040"
     CDS             complement(7220..7909)
                     /locus_tag="PK264_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRD75290.1"
                     /translation="MSRLIAAYPEHLAGVEGNELVWKDGTRMVIDDGRGAKPFETLLA
                     SPDLKDMFYAPYPVGGQAIPPAVNVDPGRVRHAAFFDKIYGDCRSGAVAANLVEIVWL
                     PRKWGRRLQVTRLNGVAARLEAVSKVLDALPARFDPYLYPSAGTYNCRPIAGTDRVSA
                     HGHGIAIDIALKHAHYWRWTKPDASGRYPYRNAIPMEIVEIFEAHGFIWGGKWYHYDS
                     MHFEYRPEMFP"
     gene            8257..9381
                     /locus_tag="PK264_05045"
     CDS             8257..9381
                     /locus_tag="PK264_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HRD75291.1"
                     /translation="MSVKDAHASSRPPCEHAGARSPYEPPGDQRQCHYGALDLGTNNC
                     RLLVARPTRRGFQVVDAFSRIIRLGEGVTSSGRLSSAAMDRTIDALRVCANKLARGRV
                     TRSRLVATEACRFAENGTEFLSRVRNEIGLHIEILSRESEARLAVSGCSGLLDGQWDL
                     ALVFDIGGGSSELIWLDASRPCGDSGAGPTAAASAIQSCVAAWTSLPIGVVTLAERFG
                     GRCVEGDCFEDMVAYVTSSLEPFEAAHRFRERIAGTRYHMLGTSGTVTTVAGIHLGLD
                     RYDRSRVDGCWLSSCDVQSVTDRLIGLSYEQRMAEPCVGKDRADLVLAGCAILEALLR
                     LWPSTRLRVADRGLREGILTTLMAEDGVHRHRQTRHGGRR"
     gene            9378..10061
                     /locus_tag="PK264_05050"
     CDS             9378..10061
                     /locus_tag="PK264_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HRD75292.1"
                     /translation="MSGGGERHLRVRLKSARKRTTSSQRWLERQLNDPYVAASKRDGY
                     RSRAAYKLIEMDDKYHILAPGHRVVDLGAAPGGWSQVAAHRVKSVEGRGQVVAIDISP
                     LEPLAGVIVLHLDFLADEAPELLRSHLRGGAADVVLSDMASPATGHTRTDHLRIMALA
                     EAAIAFAEEVLASGGSFICKVLQGGTERSLLDTLKRNFATVRHVKPPASRSDSAELYV
                     VASGFKGRS"
     gene            10234..11004
                     /locus_tag="PK264_05055"
     CDS             10234..11004
                     /locus_tag="PK264_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763039.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HRD75293.1"
                     /translation="MGDLMLWAEQLTRSFAELFGDNAQTALAVLKIIGINIVLSGDNA
                     VVIALACHGLPPRQRRMGVLLGAGAAVVLRIIFTVIIQKLLGVPWLKLIGGLLLLWIA
                     VKLLMGDDHGGDGISSGSSLFEAVKIVAIADIVMSLDNVLAIAAAAESAPEHQRIWLV
                     ILGLAISIPLVVAGSTLIMGLLSRWPVLVWAGAALLGWVAGELIATEPVIKPYVAGWA
                     TSLGLSAKSLLRVFEIGGALIVVAIAMLLKSRQATARH"
     gene            11114..12394
                     /gene="nuoF"
                     /locus_tag="PK264_05060"
     CDS             11114..12394
                     /gene="nuoF"
                     /locus_tag="PK264_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRD75294.1"
                     /translation="MLADKDRIFRNLYGFEDWGLAGARMRGCWNNTKALIEKGQEAIV
                     DEIKKSGLRGRGGAGFPTGMKWSFVPKNDPRPCYLVINADESEPGSCKDREILRNDPH
                     VLVEGALVSSFAVKAHTCFIYVRGEFIRERERLEAAVAQAYDAKLIGKDNVHGWDLDI
                     VVHHGAGAYVCGEETALLESLEGKKGQPRMKPPFPANVGLYGAPTIINNVETIAVVPE
                     ILRRGSGWFSAIGRPNNTGTKLFQISGHVMKPCVVEEAMSIPLRELIETHAGGVRGGW
                     DNLLAVIPGGSSMPVIPASECDDLLMDFDSLVKLKSGLGTAAIIVMDRSTDIIKAIAR
                     IAYFYKHESCGQCTPCREGVAWIWRIMERMAKGEAEKREIDLLFEVTKQVEGHTICAF
                     GEGAVWPIQGFLRNFRSVIEQRIDARHAGKLAAE"
     gene            12612..>13014
                     /locus_tag="PK264_05065"
     CDS             12612..>13014
                     /locus_tag="PK264_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75295.1"
                     /translation="MSDEAERVQQRRVSAHFRRFPEHLREPIKRFALDSRARSDLAVV
                     FPGAAVSLVARYCGAEAARRALALLDAERPLKEVAQTIGIPMWMRKLPPEAFALPFGR
                     LPDDEDFSRRITSYLPRSRAVAAAWLAAVSYG"
     assembly_gap    13015..13067
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13068..13781
                     /locus_tag="PK264_05070"
     CDS             <13068..13781
                     /locus_tag="PK264_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HRD75296.1"
                     /translation="PDDPEPRLAMLAAWAWFSTEIGTDARQLISTRWRPDLAFETAIV
                     GAKSWFNRLRLAALYLNRQVADPWLAGGVVRGYHVVPLLTAKDLLAESRGMHNCVDQF
                     AKRISEDRCRLFSIRTSEGVRLATVEIGQHPRETGFLAINQIKGRHNLPASVEAWQVA
                     HTWMGEQKNLCRMPPIVLDCPHVDRGAWLGLMKPYIDAKGDGRAVPRNVSVPILNQLD
                     EDLANLARRGGVTSWLFNT"
     gene            complement(13784..>14348)
                     /locus_tag="PK264_05075"
     CDS             complement(13784..>14348)
                     /locus_tag="PK264_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRD75297.1"
                     /translation="VSKTFEADAIRPVLSAGQRIFGENRVQEAKAKWPALRDEFPGVE
                     LHLIGPLQSNKAAEAVALFDCIHVVDREKIARALAAEMHKQGRRPRLLVQVNTGAEAQ
                     KAGVGPSELAGLLALCRDELKLEIAGLMCIPPVEEEPAVHFAFLAKLAREHGLAELSM
                     GMSADFATAIEQGATYVRVGSAIFGAR"
     assembly_gap    14349..14393
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14467..15102)
                     /locus_tag="PK264_05080"
     CDS             complement(14467..15102)
                     /locus_tag="PK264_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75298.1"
                     /translation="MSEASNLFGSTAGGPLTRFQLAARAIAISVPVLGAIPTGMNLYQ
                     SWKHGVPYSEVSHRLAQYDLWMKNGSCNINYREVNAQKGAKVMIGACPTTGDISIKLA
                     YASGQAKYEWIAFDTLHKTAGLLDAILPAAHAEEPKGASERLKVAQAAGAPGAEVKCQ
                     VMAAGKMIRIVGEGSKCFRETIHPFQGKLEKREEIECAKACPVDPTAPVKK"
     gene            complement(15241..16449)
                     /locus_tag="PK264_05085"
     CDS             complement(15241..16449)
                     /locus_tag="PK264_05085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD75299.1"
                     /translation="MKFGIFYELQIPRPWHASSEFDLFQNALTQISLADRLGYDYAWV
                     VEHHFLEEYSHSAAPEVFLGAASQRTERIRLGHGIFQLTTSPPARIAERIATLDLLSR
                     GRVEFGMGESASVTELEPFGVPFERKREIFEDAVRCLIPMFGDAPVAYDGPHHKFPFR
                     NVVPKTLQKPHPPLWCACSQLDTIELAGRRGFGALGFQFVSADAAKAWVHAYYNAITR
                     RLERLADYPINPNIALVSYFMCAKTDEEAQRRADGATFFQFALRFYGNAANRARPRAG
                     TVDLWQEYEAWKRADPEGQAKALRSGLIGSPETIRRKLKKFAASNVDQVILLNQAGKN
                     THEHICESLELFAREVMPEFHADEGRLKDWKSAVLAGNISLEEIDTSPFKDRFGKNAL
                     TVPPKASAAE"
     gene            16644..16946
                     /locus_tag="PK264_05090"
     CDS             16644..16946
                     /locus_tag="PK264_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75300.1"
                     /translation="MTAVLTRNEKRIVAQYLASREGYSRVAWDMLPYIAPPTGFAAYA
                     LLKEDVYAMAFAFAVLLALAIWYLAYQTRASLLFLSALRKYEDTVRALERPDADPS"
     gene            complement(16955..17488)
                     /locus_tag="PK264_05095"
     CDS             complement(16955..17488)
                     /locus_tag="PK264_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75301.1"
                     /translation="MPEVRIARLVEIDGWERLIRGIEAIFFAASGRTFSDDAERAAFR
                     ERWLGRYLQHDVSLTHVALTGDAVAGYIVGSHDDPAKAARFADLAFFGCFASLTAAYP
                     GQLHINLDARYRSRGIGGRLIEAFMADARGHGVPGVHVVTGAASRNVTFYERSSFEPS
                     CCAAWNGRRLLMLKRLA"
     gene            complement(17481..18575)
                     /locus_tag="PK264_05100"
     CDS             complement(17481..18575)
                     /locus_tag="PK264_05100"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRD75302.1"
                     /translation="MLSTIFNLAKPMLFALDPETAHEATLKSLEAGLYPRFSGPDDSR
                     LNVKAFGLTFPNPIGMAAGFDKDARVPDAVLGMGLGFAEIGTVTPLPQAGNPKPRQFR
                     LVAERAVINRNGFNNHGHKAAFERLSRRPRQGIVGINVGANKDSRDRTADYVAGAEMF
                     YDLASYLTINISSPNTPGLRDLQAPAALDELLGRVMAVRAARIAAGERHVPMVVKIAP
                     DIAEDDLPAIVERIVAHGVDAIAVSNTTLARPGLEKLAVAKEAGGLSGAPLFSRSTAM
                     LARVYQLVGRRLPLIGIGGIGKPEQALAKIEAGASVIQLYTGLVFGGPELIGQIKTHL
                     VQHCRDKGLRSIGEATGSAAEAWARRPLDA"
     gene            complement(18575..18922)
                     /locus_tag="PK264_05105"
     CDS             complement(18575..18922)
                     /locus_tag="PK264_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HRD75303.1"
                     /translation="MPRLVFKVVGQGEWSAARKIGRYEGSRDDRRDGFIHLSMPDQLA
                     GTLERHFAGLPDLVLVAVDTGRLAGALRLEASRSGALYPHLYGALDLAAVVWEKPLTL
                     GPDGQHLVPEETT"
     gene            19137..>19421
                     /locus_tag="PK264_05110"
     CDS             19137..>19421
                     /locus_tag="PK264_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRD75304.1"
                     /translation="MPDEFRVLLQTLAELLSGARRYRVPSFQRPYSWTTKEALQLLED
                     LLHWIAQATDRAHDIETRRYFLGTILLVREPADAGEPEADIVDGQQRTFLV"
     assembly_gap    19422..19493
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19494..20897
                     /locus_tag="PK264_05115"
     CDS             <19494..20897
                     /locus_tag="PK264_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HRD75305.1"
                     /translation="ALPTAAQGSAPRRHRLALRGESADHLTSLALRPGATEQEAPEGE
                     PPPGVSNMIAVREALGDALDELEDQELVALGDLIGSVCCIAVIETSHVDDAHRIFMVL
                     NDRGKPLDRKDILKAEVLGRVALDQRQRCLVIWEERERLLGVGFEVLFGHIRAIHGRS
                     GPRIIDGIRSIIAETGGAERFVDRVFAPAADALAAINHCRHEGSPHSAAICRRLRYLG
                     WLTGTEWVPATLAFWLANPDKPEVLYAFLREIDRIAYTARLLGLGGDKRIARFDRLTD
                     LVRSGRGLDTRLAPHVLTREELRNINYNLRSLHYRSQQTCKLVLMRLSDEIAGREENL
                     DPADYTVEHVLPLNPGRTSSWRTVIPDAEERARVANLLGNLVLVPRALNADAGNLDLS
                     AKLDLYFAAGRPRQPAITEQLRGLATWNADTIRARDADFASRLQRMWRLEDAPSEPAT
                     PPASGRPRRRGRARTSD"
     gene            20940..>22346
                     /locus_tag="PK264_05120"
     CDS             20940..>22346
                     /locus_tag="PK264_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRD75306.1"
                     /translation="MRRPQPLPKFLLDAIRAEVATIGHLLSPAFRFRLAWFQRAHAWT
                     ADHALLLLDSIEEAMDIAADERRWLNLNTISIVQRDGSDHVIVTDGQQRLLTLTILVA
                     ILRDFAETDTERRELHDRLFGAPTAEEPKGYYRFEPHPSTKGLLVDYVLTPGATKKAI
                     DDNDEDELSFSGRNILACRDAMWKRVKDMSVERRRALAAFLAGRCCVIVQIMASEEDA
                     RVAYCNAHSMGVHLSPADMFKAETLSVIARSARKSASRAWEQWQAKLGPEAFEELLRA
                     MLTIERKRYGKPRQLYEDLNKAFRLEEPDGAEQFVLKRLPRAAELLHAIKRADIGRAG
                     AGFAPINRRLQYLGWVTHRTWVPAAMSAIQQLGDRPDALLAFLARLDRLAYTQMVLGR
                     DGDLQQRRYVKLLQASDANDPALVVGRDEQREMLAILRSSRVVRPRFKVMLLLRINGA
                     VEGDGAPVETPMANIEHVL"
     assembly_gap    22347..22356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22357..22636
                     /locus_tag="PK264_05125"
     CDS             <22357..22636
                     /locus_tag="PK264_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HRD75307.1"
                     /translation="KTPATKSRWLTHFPNAAVRAELLDSLGNLVPLTETENQGAGNDD
                     FDRKKAIYSASVYRMTREAGAEPGWTPAIVRARTERLVAALSRSLELG"
     gene            complement(22646..>23415)
                     /locus_tag="PK264_05130"
     CDS             complement(22646..>23415)
                     /locus_tag="PK264_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HRD75308.1"
                     /translation="EDQLRNYWYADEMTRPSGGRDFKFDGLLYSNNSIFNILRSMERH
                     YSNTKGKMTIRGGVIAADNRITDCAYSAVRANAAADVQIVTNNVARVGEVALYAEFAF
                     SGAVISGNIVDGAAAGISVTNFNEGGRLAVVEGNLIRNLARREHEPVDKRGEGIAVEA
                     DATVVGNVIEAGIVVGWGRYMRDCVVSGNVVRASRVGIAITADGEAGACLVHGNLVSG
                     ASEGGVRAMRLGVLFGPDLTRQASESRRVVVAANVTA"
BASE COUNT         3970 a         7980 c         7306 g         3969 t
ORIGIN      
        1 cttgcggaag cgctcgtcgt tgttccagag cgtcggcagg caggagggcg gcagcggcag
       61 cttgatcgca agcgtgccgg tctcgcccgc cttgacgggc tttcctgtcg gatcgagcac
      121 ctggaggtcg tagcccggca tcggcaccgt gggcgagccg tacttcaccg ggagctggcc
      181 tagtccgacc ggatttcccg cgatggccca gcccgtctcc gtctgccacc agtgatcgat
      241 cacggggacg gagagcttcg cctctgccca cttgatggtc tcgggatcgg cgcgctcgcc
      301 tgccaggaac agcgtgcgga aggcgcgcag atcatagctc ttgatgagct cgccctgcgg
      361 gtcctccttc ttgatggcgc ggaaggccgt cggcgcggtg aacatcgccg cgaccttgtg
      421 ctcggcgatg acgcgccaga aggcgccggc atcgggggtg ccgacgggct tcccctcata
      481 gacgatggtt gtcgccccgt gcaggagcgg cgcatagacg atgtagctgt ggccgacgac
      541 ccagccgacg tcggatgctg cccagaacac ctcgcccggc tcgattccgt agtggttgcg
      601 catggaccac ttgagggcga ccatgtggcc gccgttgtcg cgcacgacgc ctttcggcgc
      661 gcccgtggtg cccgaggtgt acaggatgta gagcggatcg gtcgctgcga ccggcacgca
      721 gccggccttg cggccccgtg ccttggcatc cttgacgagg ccctcccagt cgtgatctcg
      781 gccggcggtc atcgccgctt cggccatcgg ccgctgcagg acgatgcagt gtttcggctt
      841 gtgcgcggag agctcgatcg ccttgtcgag cagcggcttg tagggaatga cacgctgggt
      901 ctcgatgccg caggatgcag ccaggatcgc gaccggcttg ctgtcgtcga tgcgggtcgc
      961 gagctcggcc gcggcaaagc ccccgaagac gaccgaatgg atggcgccga tgcgtgcgca
     1021 ggcaagcatg gcgatcgccg cctcggcgat catcggcatg tagacgatga cgcgatcgcc
     1081 cttcttgata ccgagatcga ggaggacgcc gccaagcgtc gcgacctcgt gcgtgagttc
     1141 ggcataagtg taggagcgag tcgtcttgga gacggggctg tcgtagatca gcgccttctg
     1201 ctggccgcgc ccgcgcagta cgtggcggtc caggcagttg tatgccgtat tgcactccgc
     1261 gcctgcgaac cagcggccgt actcgccggc atagggatcg agtaccttgt cccagaggtt
     1321 gtaccagtcg atgtcgcgcg cagcctccgc ccagaaggcg tccgggtcgg acttccaggc
     1381 ggcatagacc tcgtggtagc ggctcttcgc tgtcgacatg gtaaacgtgc tcccgaacgc
     1441 tgaaggccgg cctgcgcggc cttcccactc tcctttcagc atgggggccc cgacgcccgc
     1501 aatagtccca ccgtcgaaca tgcgaaggga cggccgtctg ggttgccccg caactttctc
     1561 acaccagagc acgatgcttg aagtggcgtc ccgccatccc atatccttga cagtcggagg
     1621 tcgccttcgg gggcctccgg gttgcagctg cctccgggcc gcaactgcca aggtcgctta
     1681 tcgaaaggac tttgcagccg tgtcgagact gtcacttctg tcgagcccgc tgctcctcgg
     1741 ctttgaggag atcgagcgcc tcatcgagcg tgctgccaag gctggcgacg gctacccgcc
     1801 gtacaatatc gagcgcatga cgcgctcgga gagcgccccc gagacgctgc gcatcacgct
     1861 cgcggtcgcg ggcttcaagc gcgacgagat cgagatcacg ctcgaggaca atcagctgat
     1921 cgtgcgcggg cgccaggacg acgacaagtc gcgcgagtac ctgcatcgcg gcatcgcggc
     1981 ccgccagttc cagcgcacct tcgtgcttgc agaaggcatc gaggtcaggt ccgccgatct
     2041 tgcgcacggc ctcctgtcga tcgacctcgt ccggcacgag cccgagcgca tgatcaaacg
     2101 catcgagatc cggggtgcga aaccctgatc tggcgagcac attttggaaa tcgaacgaat
     2161 cggaccagta tggcgtcggc gccctccttc gacaccttgc cgccatcacg cggtcgcggg
     2221 acgacgccag actgacgcct tccatgaaag gagcagcgtc atgaccgaga caggtgccaa
     2281 gcggcgcaag cccggccaga agcgccgcag ccgtgcgctg atgtcccgca gcgagctcgc
     2341 caatctcggt gacgggcatg tcgcctacat ccgcgtcatg agcgttgcgg aggcccgaag
     2401 gcgcttcccg gccgtcggga cactccccga cgtcaaggat ctctacgccc tgcactccgc
     2461 ggacggcaca ccgattgccc ttaccgacag ccacgaggcg gcgatcggcc acgccatggg
     2521 cgacgatctc gtgatcgcga gcgtgcactg agcggcggcg cattcactct atgatgggag
     2581 aggctgtagc cggttgtggc tgcggcctct tcttttggcg gcgcaccgcc gcacctcaat
     2641 cgctcttgac cgggcgatgg catggcgata gcgggacggg gctctgccca atccccgaca
     2701 tgcctccgaa tgagccaacg cgatgtctcg gcccgagaat tccagacctg aggcacgcta
     2761 tgcggagcgc gttgcgcgcg gcgagatcga gcacgatgtc gcacaggccg ccatcgtcgc
     2821 gcaactcggc gccctcggcg atgccctcgc cacctggcgc ccgcaaaagc gctggtcgct
     2881 ctcccagatg ttcggcgcca aggcgcggcc gccgcgcggc ctctacatcc atggtgctgt
     2941 cgggcgtggc aaaaccatgc tcatggacct gttcttcgat gccgtcgcga tcgagccccg
     3001 gcggcgcatc catttccacg agttcatggc cgaggtgcac gaccgcatcg ccactgcgag
     3061 gaaaacggtg gaaggggatc cgattcccca cgtcgcgcgc gagatcgcgg aaggcgcgag
     3121 tctcctctgc ttcgacgagc tgcatgtcac cgacatcgcc gacgccatga ttctcggccg
     3181 cttgttcgcc ggcctattcg ttgccgacgt cgtcgtcgtt gcaacctcga actcatcgcc
     3241 aggcgaactc taccgcaatg gcctcaaccg ccagctgttc ctgccgttca tcgccatgat
     3301 cgaggagcgc ctggacgtcg cacagctcga tgcggccaag gatttccgcc tcgagaagct
     3361 tgcgggcctg ccgctctatt tttcacctgc cgacgccgac gcccggcgcg cgctctccaa
     3421 ggcgttccag cgtctcaccg gtgtcccaca cggcgccccc ttgacgctcg acgtgaaggg
     3481 acgcgccgtc atcgttcccg aggccgcgag cggcgtcgcc cgcttcacat tcgatgagct
     3541 ttgcgacacg cccctcggct ccctcgacta cctccatatc gcgcacgcct tccacaccgt
     3601 catcatcgac agtgtgccgc tgcttgttcc cgagcgccgc gccgcggccc gccgcttcat
     3661 caatctcatc gatacgctct acgacaactg cgtcggcctc atcgtgtcgg ccgcggccga
     3721 gccgcacgag ctgcacaccg agggcaagga ggcgcagctc ttcgagcgca cggcgagccg
     3781 cctcgtcgag atgcggagcg aagcctacct cggcgagcga gcccgaaggc atgacgatct
     3841 cgctgccgaa taggcactcg ccgtttggcg aggttggcaa aatgcgcgca gtctgcctca
     3901 attgacgcac tcggggtggt tgccgatgga cgacggtcac ggtggcgtga actccccgcg
     3961 tcggatttcc tgtaggagta tatgcgtcag tcctgatatc ctgacggcgc tctgattgtg
     4021 acggaggctg gacgcacgtc cggccggcct ccctgaacga ccagggccaa ccaacgaaag
     4081 taatagcggg agggaggcgc tgccgcggag cagccaatat tgccacacat aaatcatgaa
     4141 catggcttcg tttcgccgag caagtacccg cgcgatagaa cgggacaatt ggtgcgcgat
     4201 ccttgtccat ggtgctcggc gctgctgcat ggctaccgtc tcggcggacc atgccggagc
     4261 gatacgatct gaacgtcgga aactctgcct ctagaactga actccaaaga gctgaacaat
     4321 cactcgtctg aggaacccgc cggcattgct catccagcac gccggcagaa ccaactgggg
     4381 atcgaagtat gtcaccaatg cgtaccctgt ttgccgcgct tgccgcattc ggccttgtca
     4441 gcgccgccgc cttcgcgcag ggccccgcga cccataaggt cgccatccac gtcaacgaga
     4501 acaatcccgc gacaatgaac ctcgccctca acaacgtgct gaacatcatc agctactaca
     4561 agaagaaggg tgagacggtc gccgtcgagg tcgtcaccta cggtccgggc ctgcacatgt
     4621 tccgcgccga cacgagcccg gtgaagcagc gcgtcgccca gattgcgctc gagaacccga
     4681 cggtcacatt cgccgcctgc ggtaatacgg ccgagaacat gtcgaaggcc gagaacaagc
     4741 cggtgtcgct catcagcgag gcaagggtcg tcccatcggg cgtcatccga ctgatcgagc
     4801 tgcagaaggt cggctacgct tacatcaagc cttgatgtcg taagcggcag ggacctcctc
     4861 cctgccgctt cgatctcaac tgacacgggg gatcgcccga tccggttcgg gccgcaactg
     4921 aggagagtga ctgtgatgcg ttctgcgttc aggccaatct acgggctact tgccctttcg
     4981 ctgctggcgc tggccggctc ggccatcgct caaagcccga ccgaggtgcg ccgcgatgcc
     5041 gtcaccaagg agaagatcaa ctcctggacg atcggtctcg ctgccggccg catcgagggc
     5101 acgccgctcc ggctcgccgc agagctcgcc caggccctcg acaacgagga cgccatgcgc
     5161 gtcctgccga tcgtctcgcg gggcccattc gacaacatgt acgacctgct gttcctgcgc
     5221 ggcgtcgaca tggcaatcgt ctatgccgac aatctcgatc acttccgcac gaaggagcgc
     5281 gtcccgggtc tgctcgaccg ggtcaactat atcgcccgcc tgttccccgc cgaggtgcat
     5341 ctcgtcgtcc gccccgagat caactcgctg aaggatctcg tcggcaagac gatcaacgtc
     5401 aacacggccg gaaccgcggc ggcctactcc ggccccatca tcttccagcg tctcgggctc
     5461 aacgtgcgcc tgaccaacaa cccgcaccgc gacgcactgc aggagatccg caagtccgac
     5521 aagtacgcgg gcctcctgtt cgtgacgaca aagcccgtgc cgccgctcgc cgcgccgaac
     5581 tggccggagg gcttcaagct gctgccgatc gagatgacgc cggcgctcga ggaatactat
     5641 gtgccggcct atctcgaggc cgccgagtat ccgaaactca ttcccgccgg ccagcgtgtc
     5701 acgaccatcg ccgtcccggt cgtgctcgct gtttacaact ggaacgtcgg caaggaccgc
     5761 taccggcgca tggtgcgctt tgtcgactat ctctttgata ggctgccggc actccagaag
     5821 gagccgaacc atccggcctg gaagcaggtg aacctcgccg cgaccgtgcc tggatggaag
     5881 cgctatccag cagtccagag caagctcgac gcgctgaagg gcggcgcgcc caagcgctga
     5941 cgtcgcagcc ggagacctgt gaagcgagag ccacggaaca agggagtgca tcccaacgtt
     6001 ccgtggctcg atcgttaggt accggacagc tcctcgccgc aatccgtcaa cccgccacga
     6061 ggcatcatgg catctccagg cctgggcagc cgaaggatcc aggccaagaa caagcacatt
     6121 ccggctccgc aacccaataa tcaagacagg gaggacttca tgcgtcgcca catgttgcag
     6181 ctcggtgcgg ccctcggtct cgccgcactc tccttgacca gctcggtcgc gctggcccag
     6241 gaccgcctgg ccgtattcaa ggagagcctg cgctccaagg ccaatgcgca caccgtcggc
     6301 gtcgcctcgg gcaatcccaa cggcgcctat ctctacctcg cctatgatct cagcgccgtc
     6361 ttcgacagtg aagcgctccg ggtgatcgcg gtcgttggca agggcgggca gcagaatgtg
     6421 aaggatgcgc tttacctgcg cggcatcgac ctcgccatca cgcaggccaa cgtcctcaag
     6481 cacttcaagc gcaccggcga gctcggctcc gatatcgaca agcgcctcgt ctacatcacg
     6541 cgcctctaca ccgaagaaat gcacgtcgtc gtgcaccaaa agtcaggtgt cgcgagcctg
     6601 caggagcttg ccggcaagac cgtgaatttc tccgacgccg gcagcggcac ccagctatcc
     6661 gcacgcgtcc tcttccagct ccttggcctc aatgtgaagg agatcaacgt cggacaggcg
     6721 gacgcgctgc gcatgctggc cgccggcgag atcgcggcaa ccgtcctcgt cgccgggaag
     6781 cccgccgggg cattcggccg cttggccgca gattccggct atcgcatcct gccgatcccc
     6841 tacacccagg caatggagga cgccgacttc ctgcccatcc gcctgagccg aaccgactac
     6901 gccatcattc cggatggcca accggtcgac agcctcggcg tgagtgccgt gcttgcggcc
     6961 tacaattggc cgaaaagcag cgatcgctat gcccgtatcg agcgcttcat caacgagctt
     7021 ttcacgcgcc tcgccgagtt gcagaaaccg ccgcgccatc caaaatggaa ggacgtgaat
     7081 ctctcggcaa cgctcccggg ctggacgcgc catccggcgg cccagagctg gctggaccgg
     7141 caacggtgag gctgcgcaag ggccggcctc ggccggcccn nnnnnnnnna tcaccatcgc
     7201 gccgccggcc gcctgagcgt cacgggaaca tctcgggccg gtactcgaag tgcatgctgt
     7261 cgtaatgata ccacttgccg ccccagataa agccgtgtgc ctcgaagatc tcgacgatct
     7321 ccatcgggat cgcgttgcgg tagggatagc ggccgctggc atcgggcttt gtccagcgcc
     7381 agtagtgcgc atgcttcaag gcgatgtcga tggcgatgcc atgaccgtgc gcggaaacgc
     7441 ggtcggtccc cgcgatcgga cggcagttgt aggttccagc tgacggatag aggtagggat
     7501 cgaagcgcgc cggcagggca tcgagcactt tcgagaccgc ctcgagccgg gcggcgacgc
     7561 cattgagccg cgtgacctga agcctgcgcc cccacttcct gggcagccag acgatctcga
     7621 ccagatttgc cgcgacggcg cctgaacggc agtcgccata gatcttgtcg aagaaggctg
     7681 catgccgcac gcgccccggg tcgacattca cggcgggcgg tatcgcctgc ccgccgaccg
     7741 gatatggcgc gtagaacatg tccttgagat cgggagacgc gagtagcgtc tcgaagggct
     7801 ttgcgccccg cccgtcgtcg atcaccatcc gcgtcccgtc cttccagacg agttcgttgc
     7861 cctcgacccc ggcaagatgc tcggggtaag ccgcgatcag gcggctcatg gcggtggccg
     7921 tgggcttggg gtccggtgga agcggctgtc cacctgccgt ctccgcgagg tgaagggcag
     7981 caaaggcgac cgcggccagc gcagcaatcg gccgcgacac cgcactcagc gccgaatgac
     8041 gtaaatgggc cacggtcaca gttcctcgcc gccacggtag ttcactattc ctggagccga
     8101 gctttacccc aacctgcgag ctgatacact cacggatcga ataagggacg ggaccgaagg
     8161 cgcctcgatg ggtacgcgag gtgacctccg gccgagagaa gtgcgccgaa cacgatgcaa
     8221 cgagatggcc tccagaacga cgaggagccg gccgatgtgt ccgtgaagga cgcgcacgcc
     8281 tcgtcgcggc caccctgcga gcatgctgga gcgcgctcgc cctatgagcc gccaggcgac
     8341 cagcggcagt gtcactacgg cgcactcgat ctcggcacga acaactgccg gttgctggtc
     8401 gcgcggccga cacgacgcgg cttccaggtc gtcgatgcct tctcgcgcat catccgtctc
     8461 ggcgaaggtg tcaccagttc ggggcgtctc tccagtgccg ccatggaccg cacgatcgat
     8521 gcgctccgcg tctgcgccaa caagctcgcc cgcggccgcg tgacgcgctc gcgcctcgtt
     8581 gccaccgaag cctgccggtt cgccgagaac ggaaccgagt tcctgtctcg cgtgcgcaac
     8641 gagatcggcc tccacatcga gatcctctcg cgcgagagcg aggcgcggct cgcggtctcc
     8701 ggctgctccg gcctcctcga cggccaatgg gatctcgcac tcgtcttcga catcggcgga
     8761 ggctcgtccg agctcatctg gctcgacgcc tcgcgcccct gcggcgacag tggcgccggc
     8821 cccaccgccg ccgcatccgc catacagtcg tgtgtcgcag cgtggacgtc cctccccatc
     8881 ggcgtcgtga cgctcgccga gcgcttcggc ggccgctgcg tcgagggcga ctgcttcgag
     8941 gacatggtcg cctacgtgac ctcgagcctc gagccattcg aagccgcaca ccggttccgc
     9001 gagcgcatcg cgggcacccg ctaccacatg ctcggcacgt ccggcaccgt gacgaccgtc
     9061 gccggcatcc atctcggcct cgatcgctac gaccgctcgc gcgtcgatgg ctgctggctg
     9121 tcgagctgcg acgtccagtc ggtgaccgac cgcctcatcg gcctctccta tgagcagcgc
     9181 atggccgagc cctgcgtcgg caaggatcga gccgatctcg tgctcgcagg ctgcgccatc
     9241 ctggaggcgc tgctgcggct ctggcccagc acgcgcctgc gtgtcgcgga ccgcggactg
     9301 cgcgagggca tcctgacgac gctcatggca gaggatggcg tgcatcgtca tcgccagacc
     9361 cggcacgggg ggcgacggtg agcggcggag gcgagcgtca cctcagggtc cgactgaagt
     9421 cggcgcggaa gcgcacgacc tcgtcccagc gctggctcga gcgccaactg aacgacccct
     9481 acgtggccgc ctcaaagcgc gacggctacc gtagccgggc ggcctacaag ctcatcgaga
     9541 tggatgacaa gtaccacatc ctcgcgcccg ggcaccgtgt cgtcgatctt ggcgcggctc
     9601 ccggcggatg gtcgcaggtc gcggcccacc gcgtgaaatc ggtcgagggt cgcggccagg
     9661 tggtcgctat cgatatctcg cccttggaac cgcttgccgg cgtcatcgtc ctgcacctcg
     9721 acttcctcgc cgacgaggca cccgaactcc tgcgcagcca cttgcgcggc ggtgccgctg
     9781 acgtcgtcct gtccgacatg gcctcgccgg cgacgggcca cactaggacg gatcacctgc
     9841 gcatcatggc gctcgccgag gcagcgattg ccttcgccga agaggtgctg gcatctggcg
     9901 gatcattcat ttgcaaggtc ctgcaggggg gcacagagcg ctcccttctc gacacgctga
     9961 agcgcaattt cgccactgtc cggcatgtga agccccctgc gagccgctcc gacagcgccg
    10021 aactctacgt ggtcgcgagc ggcttcaaag ggcggagttg atgctcctcc tgtcaatcgt
    10081 gcccgtcgct gatccgcttg cggcgagggt ctgcaggtcg tggaggctcg tcctgctacg
    10141 ttgacttgaa ggcagcttca cgacaaacaa gggcgcgtgg atttgtcaat ccggccatgg
    10201 cgatggcgcc ggccggagcg gaaggggaag gtgatggggg acttgatgct ctgggcggag
    10261 cagttgaccc gctcgttcgc cgagttgttc ggcgacaatg cacaaacggc actcgccgtc
    10321 ctgaagatca tcggcatcaa catcgtcctc tcgggcgaca atgccgtcgt gatcgcgctc
    10381 gcctgccatg gcctgccgcc tcgccagcga cggatgggcg tgctgctcgg cgcgggcgcc
    10441 gcggtggtcc tccgcatcat cttcacagtg atcattcaga agctgctcgg tgtcccctgg
    10501 ctcaagctga ttggcggcct gctgctgctc tggatcgccg tcaagctgct gatgggcgat
    10561 gaccatggcg gcgacgggat ctcgtccggg agcagcctct tcgaggccgt gaagatcgtc
    10621 gccatcgccg acatcgtcat gagcctcgac aacgtgctcg cgatcgccgc cgccgcggag
    10681 agcgcgcccg agcaccagcg catctggctc gtcatcctcg ggttggccat ctcgatcccg
    10741 ctcgtggtgg ccggctcgac gctgatcatg ggacttctgt cgcgctggcc agtcttggtg
    10801 tgggccggag cggcactcct cggctgggtc gccggcgagc tgatcgccac ggaacccgtg
    10861 atcaagccat atgtcgccgg ttgggcaacg tcgctcggcc tcagcgccaa gagcctgctc
    10921 cgcgtgttcg agattggcgg cgcgctgatc gtcgtcgcca tagccatgct cctcaagtcc
    10981 cgccaggcca cggcccggca ttgacggtgc gccgaggccg cgccggaggt ggtcgaaagg
    11041 cccctcgacg ccgcctcggc tcgtgctatt caggcccacg tcaaccatcg gctcaagccg
    11101 cccgtgaggc accatgctcg ctgacaagga tcgcatcttc cgcaacctct acggcttcga
    11161 ggattggggt cttgccggcg cgcgcatgcg cggctgctgg aacaatacca aggcgctgat
    11221 cgagaagggc caggaggcca ttgtcgacga gatcaagaag tccggactgc gcggccgcgg
    11281 cggcgccggc ttcccgaccg gcatgaaatg gtcgttcgtg ccgaagaacg atccccgccc
    11341 ctgctatctc gtcatcaatg ccgacgaatc ggagccaggt tcctgcaagg atcgcgagat
    11401 cctgcgcaac gatccgcatg tgcttgtcga gggagccctc gtctcctcat tcgcggtcaa
    11461 ggcgcacacc tgcttcatct acgtccgcgg cgagttcatc cgtgaacgcg agcggctcga
    11521 ggcagccgtt gcccaggcct acgatgccaa gctcatcggc aaggacaacg tgcatggctg
    11581 ggacctcgac atcgtcgtcc accacggtgc cggcgcctat gtgtgcggcg aggaaacggc
    11641 actccttgaa agcctcgagg gcaagaaggg gcagccgcgc atgaagccgc cgttccccgc
    11701 gaacgtcggc ctctacggcg caccgaccat catcaacaat gtcgagacca ttgccgtcgt
    11761 tcccgagatc ctgcgccgcg gctccggctg gttctcggcc atcggtcggc cgaacaacac
    11821 cggtaccaaa ctctttcaga tttccggcca cgtgatgaaa ccgtgcgtcg tcgaggaggc
    11881 catgagcatc cctctgcgcg agctcatcga gacgcatgcc ggtggtgtgc gtggcggctg
    11941 ggacaacctg ctggcggtca tcccgggcgg ctcgtccatg cccgtcatcc cggcctcaga
    12001 gtgcgatgac ctgctgatgg acttcgacag cctcgtgaag ctcaaatccg gcctcggcac
    12061 ggcggccatc atcgtgatgg acaggtcgac cgacatcatc aaggcgatcg cgcgcatcgc
    12121 ctatttctac aagcacgaga gctgcggcca gtgcacgcct tgccgcgaag gtgtcgcctg
    12181 gatctggcga atcatggagc gcatggccaa aggcgaggcc gagaagcgcg aaatcgacct
    12241 cctgttcgag gtgacgaagc aggtcgaggg ccacaccatc tgcgccttcg gcgagggcgc
    12301 cgtctggccg atccagggat tcctgcgcaa tttccgctcc gtgatcgagc agcgcatcga
    12361 tgcgcgccat gccgggaaac tggctgcgga gtaagatctg ccgaccaagg gcgtccgtgg
    12421 acctcctgag acccgtggcc tgcacgccgc ggcgcgggag tgatcccttg cctgccacac
    12481 aacctcccat ctcccgcccc aaacgacccg caagctactg gcgtggcttc tgaaagcctg
    12541 cctgtccagc gtcgctcatg cgccgccagc ggccacaggg gagttcagtc atgggtgtgg
    12601 ttgtacgcaa tgtgtccgac gaggccgagc gtgtccagca gcgccgagtc tccgctcact
    12661 tccgccgctt tcccgagcac ctgcgcgaac cgatcaagcg cttcgccctc gactcgcgcg
    12721 cgcgctccga ccttgctgtg gtcttccctg gcgcggccgt atcgctcgtc gcccgctatt
    12781 gcggcgcaga ggcggcgcgg cgcgcgttag ccctcctcga cgccgagcga ccgctgaagg
    12841 aggtggcaca gacgatcggc attccgatgt ggatgcgcaa gcttccgccg gaagcctttg
    12901 cgctgccctt cggccggctc ccggatgatg aagacttctc gcgccggatc acctcgtacc
    12961 tgccccggtc gcgcgccgtg gctgcggcgt ggctcgccgc cgtcagctat ggcannnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gacgatcccg
    13081 agccgcgcct cgccatgctc gcggcctggg cctggttctc gacggagatc ggcaccgacg
    13141 cccgtcagct gatctcgact cgttggcggc cggacctcgc cttcgagacc gcaatcgtcg
    13201 gcgccaagtc gtggttcaac cgcctccgcc tcgccgccct ctatctcaat cgccaggtgg
    13261 ccgatccttg gctcgcaggc ggcgtggtgc gcggctacca cgtcgtccca ttgctgacgg
    13321 caaaagacct tctggcggaa tcgcgcggca tgcacaactg cgtcgatcag ttcgcgaagc
    13381 gcatctcgga agatcgctgc cggctcttct cgatccgcac atccgagggc gtgcgccttg
    13441 ccacggtcga gatcggccag catccacgcg aaaccggctt cctcgccatc aaccagatca
    13501 agggtcgcca caacctgccg gcgagcgtcg aggcctggca ggtcgcccac acatggatgg
    13561 gcgagcagaa aaacctctgc cgcatgccgc cgatcgtgct cgactgtccg cacgtcgatc
    13621 gcggcgcatg gctgggcctc atgaaaccct acatcgatgc caagggcgat ggccgcgccg
    13681 tgccgcgcaa cgtgagcgtt ccgatcctaa accagctcga cgaggatctc gccaatctcg
    13741 cgcgccgcgg cggtgtcaca tcgtggctgt tcaatacgtg agatcagcgc gctccgaata
    13801 tggcgctgcc gactcgcacg taggtggcgc cctgctcgat cgcggtcgcg aagtcggcgc
    13861 tcatgcccat gctgagctcg gcgaggccgt gctcgcgtgc gagcttggca agaaatgcga
    13921 aatgcaccgc cggctcttcc tcgaccggcg gaatgcacat gagacccgcg atttcgagtt
    13981 tcaactcgtc gcgacagagg gccagcagcc cggcaagctc tgacggccca actccggcct
    14041 tttgagcttc cgcgccggtg ttcacctgga cgaggagccg aggacgtcga ccctgcttgt
    14101 gcatttcggc ggcgagcgcg cgggcgattt tctcgcggtc aaccacatgg atgcagtcga
    14161 agagggcgac agcctccgcc gccttgttcg attgcagcgg cccgatcaaa tgcagttcga
    14221 caccgggaaa ctcgtcccgc agagccggcc atttggcctt ggcctcctgc acgcgattct
    14281 cgccgaagat gcgctggccg gccgacagca ccggccggat tgcatccgcc tcgaacgtct
    14341 tcgagacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcggcg
    14401 atctcgccgc ggaccttgtc gagggcagca tgagccactc gtcctgctgt cgccggttcg
    14461 gccacgctat ttcttgaccg gtgccgtcgg atcgacgggg caagccttcg cgcactcgat
    14521 ctcttcgcgt ttctcgagct tgccctggaa cgggtggatc gtctcgcgga agcacttgct
    14581 gccttcgccg acgatccgga tcatcttgcc ggcggccatg acctgacact tgacctcggc
    14641 gccgggtgcg cctgcggcct gcgcgacctt caggcgctcg ctcgcaccct tcggctcctc
    14701 ggcatgcgct gccggaagga tcgcatcgag gagccccgcc gtcttgtgga gagtatcgaa
    14761 ggcgatccac tcgtacttcg cctggccgct ggcgtaggca agcttgatcg agatgtcgcc
    14821 cgtcgtcggg caggcgccga tcatcacctt ggcacccttc tgggcgttca cttcgcggta
    14881 gttgatattg cagctgccat tcttcatcca gaggtcgtac tgcgcgaggc ggtgcgagac
    14941 ctcggagtag ggcacgccgt gcttccacga ctggtagaga ttcatgcccg tcgggatggc
    15001 gccgaggact ggcacgctga tagcgattgc gcgcgccgcc agctggaacc tcgtcagggg
    15061 tccgccggcc gtgctgccga acagattcga tgcttcgctc atggctcgcc tccaagacct
    15121 atcgcgtgca accggaattc gcaggcggct ttcgcggcga cactgccgcc gaatgcgcgc
    15181 ccgttcaagt ccttcctagc gcacagaagc tgaatggcaa ctgaccaggc caccggatcg
    15241 tcactcggcg gcggaggcct tcggcggcac cgtcaaggcg ttcttcccga agcggtcctt
    15301 gaacgggctc gtatcgatct cctcgaggga gatgttcccg gcaagcaccg cactcttcca
    15361 gtccttgagg cggccctcgt cggcgtgaaa ctcgggcatc acctcgcgcg cgaacagctc
    15421 gaggctttcg cagatgtgct cgtgcgtgtt cttccccgcc tgattgagca ggatgacctg
    15481 atccacgttg gaggccgcga acttcttgag cttgcgccgg atcgtctccg gcgagccgat
    15541 gaggccgctg cgcagcgctt tggcctgacc ttccgggtcg gcgcgcttcc aggcctcata
    15601 ctcctgccag agatcgaccg tgccggcccg cggccgggcg cggttcgcag cgtttccata
    15661 gaagcggagc gcgaactgga agaatgtcgc cccgtccgcc cggcgctgcg cctcctcatc
    15721 ggtcttcgcg cacatgaaat acgagacgag ggcgatgttc ggattgatcg gatagtctgc
    15781 gagccgctcg agccggcgcg tgatggcatt gtagtaggca tgcacccagg ccttcgccgc
    15841 atccgcgctc acgaactgga agccgagcgc gccgaagccg cgccgcccgg caagctcgat
    15901 cgtatcgagc tgcgagcagg cgcaccagag cggcggatgc ggcttctgca gcgtcttcgg
    15961 cacgacgttg cggaagggga acttgtgatg cggaccgtcg taggcaacag gggcatcgcc
    16021 gaacatcggg atgaggcagc gcacggcatc ctcgaaaatc tcgcgcttcc tctcgaaggg
    16081 gacgccgaag ggctcgagtt cggtaacgct cgcgctctca cccataccga actccacccg
    16141 cccccggctc aggagatcga gcgtcgcgat acgctcggcg atccgcgccg gcgggctcgt
    16201 agtcagctgg aaaatgccgt gcccgaggcg gatgcgctcg gtcctctggc ttgcagcacc
    16261 gaggaacacc tcgggcgctg ccgaatgaga gtactcctcg aggaaatgat gctccaccac
    16321 ccaggcgtaa tcatagccga gcctgtcggc gagcgagatc tgggtcaatg cgttctggaa
    16381 gaggtcgaac tcgctactgg catgccaggg acgcgggatc tgcagctcgt agaagatgcc
    16441 gaatttcatg gtgcgtgctc gctgacttcc gcactgatgt ggcgccgctc gtccagggcg
    16501 gcccgctgga tcggcgaccg tacccggacc gtcgcggcat gaaatagcgc gcccgcgtcg
    16561 ccgtcaacca tcggcacttg ccgcgtcgcc tcatgtcgtg catgtccgat ccgcgtccaa
    16621 acggcgccgc actccacgga gacatgacgg ccgtgctcac ccgaaacgag aagcgcatcg
    16681 tcgcccagta tctcgcaagt cgcgagggtt acagccgcgt ggcttgggac atgctcccct
    16741 acatcgcgcc gccgaccggc tttgccgcct atgccctcct caaggaagac gtctacgcga
    16801 tggccttcgc ctttgcggtg ctgttggctc tcgccatctg gtatctcgcc tatcagacgc
    16861 gcgcgtccct gctgtttctg tcagcgctgc gcaagtacga ggatactgtg cgcgccctcg
    16921 agcggcccga tgccgacccg agctgagtcg cagctcaggc gagccgcttc agcatgagca
    16981 gtcgacgccc attccaggca gcgcagcagc tcggctcgaa gctgctccgc tcgtagaagg
    17041 tgacattgcg cgacgccgcg ccagtcacca cgtggacacc tggtacgccg tgtccgcgcg
    17101 catccgccat gaacgcctcg atgagacgtc cgccgatgcc gcgactgcga taccgggcgt
    17161 cgagattgat atgcagctgt cccggatatg ctgctgtgag cgaagcaaaa caaccgaaga
    17221 aggcgagatc ggcgaagcgc gcagccttcg ccggatcatc gtgcgagccg acgatgtagc
    17281 ccgcgacggc atccccggta agcgccacgt gcgtcagtga cacgtcgtgc tgcagatatc
    17341 gcccgagcca gcgctctcga aatgccgcgc gctccgcatc atccgagaag gtgcgaccgc
    17401 tggcggcaaa gaagattgcc tcgatgccgc ggatcaggcg ctcccacccg tcgatctcca
    17461 cgagccgcgc aatcctcacc tcaggcatcg agtggccgcc gcgcccacgc ctccgcagcg
    17521 ctccccgtcg cctcaccgat cgagcgcagc cccttgtcgc ggcaatgctg gacgagatgc
    17581 gtcttgattt ggccgatgag ctcgggcccg ccgaaaacca gcccagtgta gagctggatt
    17641 acgctcgccc ccgcctcgat cttggcgagc gcctgctcag gcttgccgat gccgccgatg
    17701 ccgatcagcg gcaggcggcg tccgacgagc tggtagacgc gggcgagcat cgccgtcgag
    17761 cgactaaaga gtggcgcacc cgacaagcct cccgcctcct tggcaaccgc cagtttctcg
    17821 aggcccggcc gcgccaacgt cgtattcgaa accgcgatcg catccacgcc gtgcgcgacg
    17881 atccgttcca caatggccgg caagtcgtct tcggcaatat cgggtgcgat cttcacgacc
    17941 atcggcacat gccgctcgcc ggccgcaatg cgcgccgccc gcaccgccat cacccggccg
    18001 agcagctcgt cgagggccgc cggcgcctgc aagtcgcgca ggccgggcgt gttcggcgag
    18061 gagatgttga tcgtgaggta actcgcgaga tcgtagaaca tctcggcgcc ggccacatag
    18121 tctgcggtgc ggtcgcggct atccttgttg gcgccgacgt tgatgccgac gattccctgc
    18181 cgcggacgcc gtgacagtcg ctcgaaggcc gccttgtggc cgtgattgtt gaagccgttc
    18241 cggtttatga cggcgcgctc ggcgacgagg cggaactggc gcggcttcgg attgccggcc
    18301 tgcggcagcg gcgtcacggt gccgatctcg gcgaagccga ggcccatgcc gagcaccgcg
    18361 tcggggacgc gcgcatcctt gtcgaagccc gcggccatgc cgatcggatt tggaaaggtg
    18421 agcccgaagg cctttacatt gaggcggctg tcgtctggcc ccgagaagcg cggatagagc
    18481 ccggcttcga gcgacttcaa ggtcgcctcg tgcgccgtct cggggtcgag cgcgaacagc
    18541 atgggctttg caagattgaa gatcgtgctc aacatcaggt cgtctcctca ggcactaaat
    18601 gctgtccgtc gggcccaagc gtgagcggct tttcccagac aacggcggcc aagtcgagag
    18661 cgccgtagag atgtggatag agcgcgccgc tgcgtgaggc ttcgagccta agcgcgccgg
    18721 cgagccgccc cgtatcgacg gcgacgagca ccaagtcggg gagaccggca aagtgccgct
    18781 cgagcgtgcc ggcgagctga tcgggcatcg acaggtggat gaaaccgtcg cggcgatcgt
    18841 cgcgcgagcc ttcgtagcgc ccgatcttgc gagcagcact ccattcgccc tgccccacca
    18901 ccttgaaaac gagcctcggc atcgacatgt ctgccccctc ggccggcgcg ctcgccggag
    18961 actgcaacag cggcaccatc gtcgctattg aaatggcctt attccgacaa ggatcaatag
    19021 caccgcttcg tcgatcggct gctgcatgct aatcaggcga gctgcctgtc cgatcgtcat
    19081 gacgttgtca ccgtgatgtg tcgggtccca tttccatcga catgcccacg tgccgcatgc
    19141 ccgacgagtt ccgagtcctc ctgcaaaccc tcgccgagct gctgtccggg gcgcggcgct
    19201 atcgcgtgcc gtcgttccag cggccctatt cctggacgac gaaggaggcc ttgcagctcc
    19261 tcgaggacct gctgcactgg atcgcccagg cgacggatcg cgcacacgac atcgagacgc
    19321 gccgctactt cctcggcacc atcctgctcg tgcgcgagcc cgccgacgca ggcgagccag
    19381 aagccgatat cgtcgacgga cagcagcgca cgttccttgt annnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcgctgc
    19501 cgaccgccgc gcaaggaagc gcgccgcgcc ggcatcggct cgcgctgcgc ggcgagagcg
    19561 ctgatcacct gacgagcctt gcactccggc ccggggccac cgagcaggaa gctcccgagg
    19621 gcgagccgcc gccgggcgtc agcaatatga tcgccgttcg cgaggcgctc ggggatgccc
    19681 tggacgagct cgaggaccag gaactcgtcg ccctcggcga cctcataggc tcggtctgct
    19741 gtatcgcagt catcgagacg tcccacgtcg acgatgccca ccgcatcttc atggtcctca
    19801 acgaccgcgg caagccgctc gaccgcaagg acatcctgaa ggccgaggtg ctgggccgcg
    19861 tcgcgctcga tcagcgccag cgctgccttg tgatctggga ggagcgcgag cgcctgcttg
    19921 gggtagggtt cgaggtgctg ttcggccaca tccgcgcaat ccatggccgg agcggacctc
    19981 gcatcatcga cggtatccgc tcgatcattg ccgaaacagg cggcgccgag cgcttcgtcg
    20041 accgggtgtt cgcgccggcg gccgacgctc ttgctgcgat caatcattgc cggcacgagg
    20101 gcagcccgca ctccgccgcg atctgccgcc gccttcgcta cctcggctgg ctcacaggca
    20161 ccgaatgggt acccgcgacg ctcgcgttct ggctcgccaa tcccgataag ccggaggtgc
    20221 tctatgcatt cctccgcgag atcgaccgta tcgcctatac ggcccgcctg ctcggtctcg
    20281 gaggtgacaa gcgcattgcg cgcttcgatc gcctgaccga tctcgtgcgt tccggcaggg
    20341 gactcgacac gaggctcgcg cctcatgtgc tgacgcgcga ggaactgagg aacatcaatt
    20401 acaacctccg ctcgctccac tatcgcagcc agcagacctg caagctcgta ttgatgcgct
    20461 tgagtgatga gatcgcgggc cgcgaggaga acctcgatcc tgccgactac acggtcgagc
    20521 acgttctgcc gctgaacccg ggccggacca gcagctggcg gaccgtgatc cccgacgccg
    20581 aggagcgggc acgtgtcgca aacctcctcg gcaacctggt gcttgtaccc cgtgcgctca
    20641 acgcggacgc cggcaacctc gatctctcag ccaagctcga cctctatttc gccgccggcc
    20701 gcccgaggca acccgccatc acggagcagc ttcgcggact tgccacctgg aacgccgaca
    20761 cgatcagggc ccgcgatgcc gactttgcct cgcggctcca gcgcatgtgg cgactggagg
    20821 atgcgccgag cgagccggcc actcctcccg cgagcggtcg tccgcgccgg cggggacgtg
    20881 cacgcacttc ggattgacgc gccgtaattc ccagcctttt ccttccggga tcaccaccga
    20941 tgcgccgccc gcaaccgctg ccgaaattcc tcctggacgc cattcgcgcc gaggtcgcga
    21001 ccatcggtca cctgctgtcg ccggcattcc gcttccgcct tgcctggttc cagcgcgcgc
    21061 atgcctggac cgcggaccac gccctcctcc tgctcgacag catcgaggag gcgatggaca
    21121 ttgccgccga cgagcgccgc tggctcaacc tcaatacaat cagcatcgtc cagcgcgacg
    21181 gcagcgacca cgtcatcgtg acggatggcc agcagcgact tctgacgctt actatcctcg
    21241 tcgccattct gcgcgacttc gccgagacgg acaccgagcg gcgcgagctt catgaccggc
    21301 tgttcggcgc accgaccgcc gaggagccca agggctacta ccgcttcgag ccgcacccat
    21361 cgaccaaagg gctgctcgtc gactacgtcc taacgcccgg ggccacgaag aaagcgatcg
    21421 acgacaacga cgaggacgag ctatccttct cgggccgcaa catcctcgcc tgtcgcgacg
    21481 ccatgtggaa gcgcgtcaaa gacatgtccg tcgagaggcg ccgggcgctt gccgcgttcc
    21541 tcgccgggcg ttgctgcgtc atcgtgcaga ttatggccag cgaggaggat gcccgcgtcg
    21601 cctactgcaa tgcccacagc atgggcgtgc acctgagccc ggccgacatg ttcaaggccg
    21661 agaccctttc ggtgattgcc cgctcggcac gcaaatcagc ctcgcgcgcc tgggagcagt
    21721 ggcaggcgaa gctcggaccg gaagccttcg aggagcttct ccgggccatg ctgacgatcg
    21781 agcgcaagcg ctacggcaag ccgcgccagc tctacgagga tctcaacaag gccttcagac
    21841 tcgaggagcc ggacggcgcc gagcagttcg tcctgaagcg gctgccgagg gccgcggagc
    21901 tgctgcacgc catcaagcgg gccgacatcg gccgggctgg cgccggcttt gccccgatca
    21961 accgccgact ccagtacctc ggctgggtca cgcatcggac gtgggtgccc gcagccatgt
    22021 ccgcgatcca gcagctgggc gatcggcccg acgcgctcct cgccttcctc gcgcgcctcg
    22081 accggctcgc ctacacccag atggtacttg gccgcgatgg cgacctgcag cagcgccgct
    22141 acgtcaagct gttgcaggcg agcgacgcca acgatccggc gctggtggtc ggtcgcgacg
    22201 agcagcgcga gatgctcgcc atcctacgca gcagcagggt ggttaggccc cgcttcaaag
    22261 tgatgctgct cctgcggatc aacggtgccg tcgagggcga tggtgcgccg gtcgagacgc
    22321 cgatggccaa catcgagcac gtgctgnnnn nnnnnnaaag acccccgcca cgaagagccg
    22381 ctggctgacg catttcccca atgccgccgt gcgcgccgag ctactcgaca gcctcggcaa
    22441 tctcgtgccg ttgaccgaga ccgagaacca aggagccggc aacgacgact tcgaccggaa
    22501 gaaggccatc tactccgcct ccgtctaccg gatgacgcgc gaggccgggg ccgagcctgg
    22561 ctggaccccg gcaatcgtcc gcgcgcgcac cgagcggctc gtcgccgcgc tcagtcgcag
    22621 cctggagctc ggttgaggca gcatctcagg ccgtcacatt ggccgcgacg acgacgcggc
    22681 ggctctccga ggcctggcgc gtcagatcgg gcccgaagag cacaccgaga cgcattgcgc
    22741 ggacgccgcc ctcgctcgcg cccgagacga gattgccatg gacgaggcaa gcccccgcct
    22801 cgccgtccgc cgtgatggcg atgccgacgc gcgaggcccg gacgacgttg ccgctcacca
    22861 cgcagtcgcg catgtacctg ccccagccga cgacgatgcc ggcttcgatc acattgccga
    22921 cgacggtcgc gtcggcctcg acggcgatgc cctcgccgcg cttgtcgacg ggctcgtgct
    22981 cgcgccgcgc gaggttgcgg atgaggttgc cttcgacgac ggccaggcgg cccccttcgt
    23041 tgaagttcgt gaccgagata ccggccgcgg caccgtcgac gatgttgcct gagatcaccg
    23101 cgcccgagaa cgcgaactcg gcatagagcg ccacctcacc gacgcgcgcg acgttgttcg
    23161 tgacgatctg cacgtcggcg gccgcgttgg cgcgcacggc cgaataggcg cagtccgtga
    23221 tgcggttgtc cgccgcgatc acgccgccac gaatggtcat tttgcctttc gtgttggagt
    23281 agtggcgctc catgctgcgc aggatgttga agattgaatt gttgctgtac aggaggccgt
    23341 caaacttgaa atcacgaccg ccactcgggc gcgtcatctc atcggcgtac cagtagttgc
    23401 gcaactggtc ttccg
//
LOCUS       DAORNH010000030        23214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1690_length_23214_cov_2.499331, whole genome shotgun sequence.
ACCESSION   DAORNH010000030 DAORNH010000000
VERSION     DAORNH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23214
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1690_length_23214_cov_2.499331"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>331
                     /locus_tag="PK264_05135"
     CDS             129..>331
                     /locus_tag="PK264_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRD75309.1"
                     /translation="MARALGAGLRPVLRHPVVIENVAGAGGTIGMDRAAKAKADGYTL
                     VVTSTGSVALGPQMGVGDPDHFRS"
     assembly_gap    332..376
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <377..958
                     /locus_tag="PK264_05140"
     CDS             <377..958
                     /locus_tag="PK264_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRD75310.1"
                     /translation="VGPSSPDKSLTDFVTSARSGKAPSIGTLGAGSASHLAAFAFGRA
                     TGINPTLVPYRSSSAVLEDLRAGHIDAALLLPLMPKDALAAMQLRALAVTSKAHSHLL
                     PDVPRITELTPFIFDLASWIGIAAPAGTPKGVLDKMNILFRWAAATPEVRKAMEASGV
                     TAHSLDVDETNRLIAFDTVIWAEIAKEAGLKPQ"
     gene            complement(974..2920)
                     /locus_tag="PK264_05145"
     CDS             complement(974..2920)
                     /locus_tag="PK264_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75311.1"
                     /translation="MVVRETALKAIGLIVLAGLIGSEPAMAQAARIEWRVDNPFRFFT
                     NPADTNVHRSTFAALKPDEARTPILSAERALAERHPDGWAATMFRNTCWNAVTNRHEC
                     PADPDYLNPRRHAVLLTLKGAEEDVVQCTWLVTPLEPAGRTRDAEGGSATQSCAEPLR
                     FDVPYPGGADVRVEVGGQEVARAIVRVSDLLIVGMGDSFASGEGNPDVPVRFSRERAA
                     DYGKPQKGKNSDTALVGYPARVGPWKQIGDRAFIDENARWLDQACHRSLYSWQLRAAL
                     QIAIENPHRAVTFVGYACSGAEITAGLFLRYKGHEWVPNPPDLSQISAVAQAQCDRRS
                     APDFDLPEAYHMKEQVPELKGGLVLKKCDPAHARRIDLLLVSIGGNDIGFSRLVANAV
                     LDDQSTLRRLGGWFGQVHGFKEAGDALDRLDDRYKAFNRAVHSLLHIPWGQSDRVIFA
                     AYPPLALLEDGKSVCPDGRAGMTVVPEFRMSEEKATESGVAAERLNDIMIASARFHGW
                     SLADAHRRDFQGRALCAGFIEDSLSTADDLRIPRLVDGAWQPFNPADWKAYISRQRWF
                     RTPNDAFMTGNFHVSQSLLQKTLKLQTFSWFQLILASTYSGAFHPTAEGHAAIADAAV
                     ARARVVLDKYARAGGADRRTSGAQ"
     gene            3121..4623
                     /locus_tag="PK264_05150"
     CDS             3121..4623
                     /locus_tag="PK264_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRD75312.1"
                     /translation="MLHKPRADLKPNTADFERMAQVKPTGFREYDARWLFPSEINLMG
                     LNAIGLGIATLAAERGVPKRFVVGHDFRWYSGAVKQALMTGLLAGGAEVHDIGLALSP
                     MAYFAQFALDVEGVAMVTASHNDNGWTGIKMGIQRPLTFGPDEMTALKSIVLEGRGQT
                     RAGGRYIFVPDLADRYVKSLTDRPKLKRRLKVVAACGNGTAGAWAPKVLEAIGCEVIP
                     LDCELDHTFPRYNPNPEDMKMLHAMAEAVLRHKADVGLGFDGDGDRCGVVDNEGHEIF
                     ADKVGVLLARDISAKHPGAKFVADVKSTGLFMTDPVLKAQGAETVYWKTGHSYMKRYT
                     HEIKALVGFEKSGHFFFQPPLGRGYDDGLVAAIAVCDMLDRAGTATLADLVRGLPKTW
                     QSPTMSPHCGDEVKYGVVDRVVKHFTGLKERGQRVAGQPVRELITVNGIRIVLEDGTW
                     GLVRASSNKPELVIVVESPVSEAKMRSIFAEIDGFLSSKCPEVGAYNQKI"
     gene            complement(4666..5691)
                     /gene="dusA"
                     /locus_tag="PK264_05155"
     CDS             complement(4666..5691)
                     /gene="dusA"
                     /locus_tag="PK264_05155"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HRD75313.1"
                     /translation="MQTQSHRFCVAPMMDWTDRHCRYFHRLLTREALLYTEMVTADAI
                     LHGKRNRLLAYSPEERPVALQLGGSDPLKLARAARIGADFGYDEVNLNVGCPSDRVQE
                     GRFGACLMAEPTLVADCVAAMRASVRLPVTVKCRIGIDDQDSEADLDRFVTTVSAAGC
                     STFIVHARKAWLAGLSPKENREIPPLDYSRVHRLKAAHPGLTIVLNGGIGSLGAALPH
                     LEDLDGVMVGRAAYQTPYLLADVDRRIHGATAAPPDREAVLEALVPYAACHVAAGGRL
                     ASITRHILGLYHGEPGGRLFRRHLSENAVRPGAGVEVLEAASDLMRSRRRTGVPDAVA
                     ASAGGEG"
     gene            5874..>6789
                     /locus_tag="PK264_05160"
     CDS             5874..>6789
                     /locus_tag="PK264_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD75314.1"
                     /translation="MATFLKLPSGRWRAQVRRKGRSLSQTFPTKALAEEWARDQETSL
                     DKGQAPPLPKPRSPSISHVQPRACETLASLIDLHLADLAELNRGIGRSKEHTLFRLRE
                     EIGKTRVDAITREFLVDFAKRRSKAGAGPVTISIDISFIGTVMQHAAAVHGVRVPIDE
                     LRLGRIALHRLGLVAKSAERDRRPSPKEIEQIIETANANPRQIIPLGRIVPFAIATAL
                     RQDEISRVLWEDFNPEKLTLKVRQRKHPRLKTSNDQTIPLVVDTGYDPVALINVQGER
                     TGRQTGFIFPYDGRSVGAAFRRVCRDLGI"
     assembly_gap    6790..6816
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7143..>7920
                     /locus_tag="PK264_05165"
     CDS             7143..>7920
                     /locus_tag="PK264_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HRD75315.1"
                     /translation="MILTDNETKVDLLNNEAIATTIIKLLRDRPEQPVTIGVHGDWGA
                     GKSSVLEMIEAGLDGQDKVLCLKFNGWRFQGFEDAKIALIEGIVTGLIEKRPALTQAA
                     EAVKEVFKRIDWLKVAKKAGGLAFTAFTGVPTPDQLRDLVGVLEGMAADPGKLLTKEN
                     LEGAIEGVKDVIRPGGDTTNVPKEIAAFRKAFDGLLDKAGIEQLVVLIDDLDRCLPDT
                     VIETLEAVRLFVFTSRTAFVVAADEAMVEYAVRKHFPDLPD"
     assembly_gap    7921..7930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7931..>8180
                     /locus_tag="PK264_05170"
     CDS             <7931..>8180
                     /locus_tag="PK264_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTPase KAP"
                     /protein_id="HRD75316.1"
                     /translation="GPQTYARNYLEKLIQVPFRIPSLGETETRIYVTLLLVGMELGEK
                     DPAFDALIKAAREKLKRPWKVSALDADAVKQALGDKSTR"
     assembly_gap    8181..8294
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8295..9114
                     /locus_tag="PK264_05175"
     CDS             <8295..9114
                     /locus_tag="PK264_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTPase KAP"
                     /protein_id="HRD75317.1"
                     /translation="RTAEARGFGVDVKLPVLAKLMLAERFLPRLFDQIAASAATAADG
                     RCPDLAALEAAPGRSDREAGDRVSDDATAGAAKPKVKPGLAKSSEPSALGEWSSSPAV
                     RAWASVPVALGGEDLRPYLFVAKDRKDYFGTTSALGHLGAVVEQLFGGKFAVQALEAD
                     LKRLAPPEAAEVFEAVRTRIVGGGEFETEPPGIPGLSVLVKAHPSLQGNLLDFLEALP
                     RSKLGPWVCSGWDSALPDQNHSNRFTKLLEAWSKEGGKLLKAAATGVHKTRGGR"
     gene            9489..10001
                     /locus_tag="PK264_05180"
     CDS             9489..10001
                     /locus_tag="PK264_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75318.1"
                     /translation="MADAVERGQTEALRALDLVALAGRPIEEIFLGLVDYICPDGGPI
                     DEGIAREAFIETITDLAGAGITDMNDFTAAQMQTVFELFATNAIEARLCNDIGAKTII
                     LPSDAREAMRVQQQLHDFIRRAVADALSSASAIRGALSPARALQFVDGIYQQAFGILQ
                     IMGEAEADTP"
     gene            9998..>10579
                     /locus_tag="PK264_05185"
     CDS             9998..>10579
                     /locus_tag="PK264_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75319.1"
                     /translation="MNRHILLGHFGAADRPRFTPGAEEVATRIDLLNGQDSLDHGIGR
                     ALGDLSRLGVRPSELGIDVLILAAHVHAADTRISRGIVAQDGWTREIRLLVPVSDPDR
                     WRAAAPVFTRMLKFLTGDRWELMFRARPRRHRTLAPPSPTPLLPAPFDHLALFSGGLD
                     SLIAAIDCLQAGRTPLLISHAGEGATSDAQNTVF"
     assembly_gap    10580..10623
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10624..11374
                     /locus_tag="PK264_05190"
     CDS             <10624..11374
                     /locus_tag="PK264_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75320.1"
                     /translation="RLRLWMNFPDGMVDETSSEDTTRGRSFLFFSLGVFAGTGLSHPF
                     TLNVPENGLIAVNVPLDPLRLGALSTRTTHPFYIARWNEALSALGMNGQIVNPYWNKT
                     KGDMTAECRHSALLKKLVASSLSCASPTKGRWQGHGTQHCGYCVPCIIRRAALLRGLG
                     PGNDPTTYTLADLTASSLNTEQSEGVQIRSFQLAVERIRARPSLAPLLIHKPGPLFDE
                     TTQAQAELAEVYRRGLDEVAALLAGVRTAPE"
     gene            11371..12129
                     /locus_tag="PK264_05195"
     CDS             11371..12129
                     /locus_tag="PK264_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRD75321.1"
                     /translation="MSDPLQARGVDFHCHLDLFPDFEAAIARAERAQVYTLSVTTTPK
                     AWKRNLELTKDTRFVRAALGLHPQLAAERADELPLWEALLDQTRYVGEVGLDAGPKYY
                     RSLDRQKLIFGAILRRCAGTGGKILSVHSIRSVPIVLDMIAQYLPPGRGKVVLHWFTG
                     TKAEAARAATMGCYFSVNAAMARSERGRAIVSSIPPHRILTETDGPFTQSAGRPAEPA
                     DALQTVESIASVRHLPIKDATNLVLDNLRDLLTV"
     gene            complement(12312..13661)
                     /locus_tag="PK264_05200"
     CDS             complement(12312..13661)
                     /locus_tag="PK264_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HRD75322.1"
                     /translation="MPTQCSQGEFDLGRSGGRKLVGAFDGGAITSNGGVVLLAAADRV
                     TGLGRRLARCFTDLRVADAVRHSVPDLLRQRIFGIALGHEDLIDHDALRDDPALAAVL
                     EKPGDRLAGKSTLNRLEHAGKIGSDRHHKLDHDAEAIERLIVDLFLDAQDRPPSRIVI
                     DLDATDDPVYGEQEGRHFHGYYDCYCYLPLYVFCGRHLLAAKLRSSSLDAAHGSVAEV
                     ARIVAQIRIRWPATTIVIRADSGFCRDDLMTWCEANDVQYVLGLAGNTRLVRKLAPEM
                     RRARRRAKQSGQAARVFTDFEYRTRMSWSARRRVIGKAEWTNGAANPRFIVTNIHAAF
                     GPARFLYEDVYCQRGEMENRLKECQGDLFADRTPAPTMRANQLRLWLASFAYVLMCAV
                     RRIGLAGTKLAAATCGTIRLKLLKIGARVTISVRRVKLAFATACPDREVFMLAARRL"
     gene            complement(13901..14293)
                     /locus_tag="PK264_05205"
     CDS             complement(13901..14293)
                     /locus_tag="PK264_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD75323.1"
                     /translation="MSNYFSKPHYRQRAELQSGDCTAELDQPSDAGVSGRDASTARTT
                     VDHTLEARPLPAVRELDPDRLYTGKQLARVIGISHRTLERWRSQGRGGPPYVKPDGHT
                     FRRGRVTYHGADVLAWLARGRVERGKGD"
     gene            complement(14336..14740)
                     /locus_tag="PK264_05210"
     CDS             complement(14336..14740)
                     /locus_tag="PK264_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75324.1"
                     /translation="MSDNIICRTRRFLATTTHVTSRSSTFTLANVEHVKLRRPWLPFA
                     VIGAGGLAATAAVFTRELYDHELIGIWSFGAAAIVASFRIGRLQLISLALHDQDAVVW
                     GPIGELRAVKSAIETALIARSSASSDPPRLEA"
     gene            complement(14752..15150)
                     /locus_tag="PK264_05215"
     CDS             complement(14752..15150)
                     /locus_tag="PK264_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75325.1"
                     /translation="MSQKPSSNLSAFAAAGALLIAWWMAPSATAPDVVRAYGTFTSGM
                     FAVVGAVLAIQAIKTAERRPSPWASMLGRVAWALRPRGWIIAWAAIFVAVAVAGTPHL
                     AVSYAPRPCIHAGLKGFVTTSGPCPWFRWL"
     gene            complement(15155..>15595)
                     /locus_tag="PK264_05220"
     CDS             complement(15155..>15595)
                     /locus_tag="PK264_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75326.1"
                     /translation="KEALAGLVAYGAIAWLLAGPISERLAEREHIPACIAGVSLGSDR
                     GASATLQDEVARRALKQLPEPVADVIQLLRDAKRGLRNAETASVGPARCRCLAQLALG
                     QTSVRFEHAIYVGTLKLIAPPSTTSSGFRAAMAAADPGRTCQQE"
     assembly_gap    15596..15617
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15618..15866)
                     /locus_tag="PK264_05225"
     CDS             complement(<15618..15866)
                     /locus_tag="PK264_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75327.1"
                     /translation="MRLGTRHQWAWAIAGVVINVLHYVVLGLLAGLAVVGLLVFLTHD
                     SDHRAIRARIDEVIEVWTAPRGAARKCSDVRSLDHSGRA"
     gene            complement(15863..17029)
                     /locus_tag="PK264_05230"
     CDS             complement(15863..17029)
                     /locus_tag="PK264_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75328.1"
                     /translation="MPRPPKTQISPYETRRKRRDRTLSFVQMMVWTLTGGLVLLASFE
                     LIEPDAGGFAANLKRVLATLAITFGGYSVSRLAIVRGAPLAAQGSVLAGAASALTFAV
                     LTPTIVVLSFLALSAPEVRKTDALEKCQLIRQAASQLDEVVLARTALEKSIKAIGVDL
                     QARAHGEATRGRESGKKSVTGRPQPVTTLYEDARRYAGLAEDQLARGGAERIAAVRDL
                     ASVADDCERLISDPKKWRKEGSAAVGAIYDRAGGRLRELAQAAPLSGVRSLSDQLRTL
                     ASSDTIDKGAIEDARRVVRGHSDRLDRELANLPRMPDALPPMAASPVLVAAFEPSKFA
                     GTWHNLLIALLVEGGSPLLWMYAFVHAQRQNRDDDLDDDGDDDDVGPPNGRAVP"
     gene            17180..18019
                     /locus_tag="PK264_05235"
     CDS             17180..18019
                     /locus_tag="PK264_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75329.1"
                     /translation="MSSMEPDPALAAALCKLITTMRTPKRKWSVAAIERALIRNYQDY
                     GAPARGLSEPVITDAKEGRPISWKTKDILRKFIHVNAPGLLTPLIPPVDLDLGTFFGT
                     GTSEMTRAGRRIVGTYQTYARSTSMPAYLRRGRMRFDYVDDDDKQRIAVVEEQLRPAI
                     EGREEWPLRWDGFAAPRGRSYFVVMRIPEDAYGPGTASLGIIDVATSGPGVVREAIFH
                     GLQYDGEKPGYLKFTCLLMRTQADCIMDYVKLVDLTDKEQFIIDALCLAEEIEKLRTA
                     RHR"
     gene            18034..18375
                     /locus_tag="PK264_05240"
     CDS             18034..18375
                     /locus_tag="PK264_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75330.1"
                     /translation="MFGEIAGPVEFWTRADFTEAELANDSRLRGYGNRLLGSDYNHWE
                     QFPKFSPEEIARRRLEMVQAAMRAGHTEFAMMILNGPDKLPQFRARWIRGLWPVPGGP
                     WGAPKFWEKYL"
     gene            complement(18382..>18824)
                     /locus_tag="PK264_05245"
     CDS             complement(18382..>18824)
                     /locus_tag="PK264_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75331.1"
                     /translation="GVLLTALGAIGAYAVARMIGQLAFRHRFWARQVTDRTLEVRFHR
                     RVVTHGGQTYPRGPALRFTAVPHRDGRHEDRAERHAERLLTTTYREAWQVWLQHGEVF
                     VLLADVSDEQGASAIARRLQAVDEVVSRAGQTADFSNTGRLRPH"
     assembly_gap    18825..18839
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18998..20509)
                     /locus_tag="PK264_05250"
     CDS             complement(18998..20509)
                     /locus_tag="PK264_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HRD75332.1"
                     /translation="MLERAGRELTLEEQSMPMAAIMPPAAIAASQALRHDSAKIFLGL
                     VGADFEDRDFGERRERVAVDGMPIGIGDDRHMVTVAGSRSGKGRSVIVPTLLTYRGAM
                     LVIDPKGELATMTARARVEKLGHRVVVLDPFKVTSGFAANLRARFNPMTILVTESPTL
                     IEDAGLIADAIVIPAENQRDPHWEESARNLIEGLILHVATCPLYEGRRHLGAVRDLLA
                     FRETAIAGKKGKAADRLRAEMEANDAASGMIIDASVDFFEKPDDERGSVLSTARRHAR
                     FLSYPAIHDCLAGHDIDLVDLKREAVTLYLCLPAMRLGTCARWLRLFVNLALAAFEAE
                     SAKPELPVVLCLDEFATLGKMRAIEDAAGQIAGFGVKLWPILQDLGQLKSLYAERWQT
                     FLGNAGVIQCFANSDQFTLEYVSRRLGTTSLIVRNKGEVSDQDQTRTDRRGLSWQTTT
                     QPLLTAEEIARLFGRDDAYLRQLVIWSGYDPIILSRAKYDTHALFAGDFDAVS"
     assembly_gap    20557..20571
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21001..21249
                     /locus_tag="PK264_05255"
     CDS             21001..21249
                     /locus_tag="PK264_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75333.1"
                     /translation="MPTKNEPKNRPQFRVCFARKTGVDDKGGDVLGSAQQIGSVWSRS
                     GGRGSILRFDHIPIELTQHNGVLFLFPTESDNDERKGY"
     gene            21249..21953
                     /locus_tag="PK264_05260"
     CDS             21249..21953
                     /locus_tag="PK264_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75334.1"
                     /translation="MQTLLPFMTHTTPARLPSGAGARHRAQPGRAPHEFYPTPPAAIR
                     ALLAAERFDGSIWEPACGDGAISKELIAHGYDVTSTDLVDHGYGRHGVDFLATVFPRG
                     RNIVTNPPYGSGLADRFLRHALSLTRITGGRVAMLLNITSLCSPTRHDSFIRRPPSVL
                     YALDDCVCWPSGKPALATRHTLNHRYVWAVWEPETVPVTTTAFRWLATAPFTDDAIRT
                     ARRSTRYTSTRTKETA"
     gene            21950..22429
                     /locus_tag="PK264_05265"
     CDS             21950..22429
                     /locus_tag="PK264_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75335.1"
                     /translation="MNHYIARFRVYCGKHGFDQIALFACRDAPEHYLRAIMRGWVPGA
                     TAPPSGDPHSAAVVADGIVIASTFQIKAINRDTWEMLTTLVPERGPRVECFDASLPKR
                     RYAIEYRTEGLHEIAVEATSADHAAILAYIAHDCRKPAPTEIDEEAEVTKVREVPHA"
     gene            22637..22921
                     /locus_tag="PK264_05270"
     CDS             22637..22921
                     /locus_tag="PK264_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75336.1"
                     /translation="MIVMPPAHSFRCARRWTTFERLFAPIARDDDSLLWDAREIAFAV
                     EDPRHWWTVVEGDRGGLYTVAGWHLVNRIGHLCCQHAWGGRPGDHAAYRY"
BASE COUNT         4085 a         7603 c         7225 g         4009 t
ORIGIN      
        1 cgggcgtcac gcctgcgccc gaaaaggcag agacatcggc gcccatcgcc aggccctcac
       61 ccggcagctt cccggcgcga tcgatcacgc tggtcgtccc gttcgccgcc ggtggcgcaa
      121 ccgatacaat cgcacgagca ctcggagcgg ggttgcgacc tgtgcttcgc catcccgtcg
      181 tcattgagaa cgtggccggc gcgggcggca cgatcggcat ggaccgcgcc gccaaggcga
      241 aggccgacgg ctatacgctc gtcgtcacct cgacaggatc ggtcgcgctc gggcctcaaa
      301 tgggagttgg agatccggat catttccgct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnngtcg gcccgtcgag cccagacaag tcgcttacgg acttcgtgac
      421 cagtgctcgc tccggcaagg cgccttcaat cggcactttg ggagcgggaa gcgccagcca
      481 tctcgccgcc ttcgcgtttg gaagggccac ggggatcaat ccgactctgg tgccgtacag
      541 gtcatccagc gccgtgttgg aggacctgcg cgccggccat atcgacgccg cactgctcct
      601 tccgctgatg ccgaaggacg ccctggctgc catgcagctg agagcgcttg ccgtgacgag
      661 caaggcgcac tcgcacctgc tcccggatgt gcctcgcatc accgagctca cacccttcat
      721 cttcgacctc gcgagctgga tcggcattgc ggcgccggcg ggaacgccga agggcgtgct
      781 cgacaaaatg aacatcttgt tccgatgggc ggccgcgaca cccgaagtcc gcaaggccat
      841 ggaggccagc ggagttacgg ctcacagcct cgacgtggac gagaccaatc gcctgatcgc
      901 ctttgatact gttatttggg ccgaaatcgc caaggaagcc ggcctgaagc cgcaatgaac
      961 cgccgggcga tgcctactgc gccccgctcg tccgccggtc cgcgccgccc gcgcgagcat
     1021 acttgtcgag cacgacgcgc gcccgcgcca ctgccgcgtc cgctatcgcc gcgtggcctt
     1081 ccgcagtcgg gtggaatgcg cccgagtagg tcgaggctag gatcagctgg aaccacgaga
     1141 acgtctgcag cttcagggtc ttctggagca gcgactggct cacatggaag ttccccgtca
     1201 tgaaggcgtc attgggcgtg cggaaccagc gctggcgcga gatgtaggcc ttccagtcgg
     1261 ccgggttgaa cggctgccag gcgccatcga cgaggcgcgg aatacgcagg tcgtcggcag
     1321 tggagagcga atcctcgatg aagcccgcgc agagcgcgcg cccctggaaa tcccgccggt
     1381 gcgcgtccgc gagcgaccag ccgtgaaaac gtgccgaggc aatcatgatg tcattgagcc
     1441 gctcggcggc aacgccgctc tcggtcgcct tctcttcgct catgcggaac tcagggacga
     1501 ccgtcatgcc ggcgcggccg tccggacaga ccgactttcc gtcctcgagg agagcaagcg
     1561 gcgggtaggc agcgaagatc acccgatccg actgccccca cgggatgtgc aggaggctgt
     1621 gcacagcgcg attgaaggcc ttgtagcgat catcgagacg gtcgagggcg tcgcccgcct
     1681 ctttgaagcc gtgcacctgg ccgaaccagc cgccgagtcg gcgcaaggtc gactggtcgt
     1741 cgaggaccgc gttggcgacg aggcgggaaa aaccgatgtc gttgccgccg atggagacga
     1801 gcaggagatc aatccggcgc gcgtgcgccg gatcgcactt cttgagcacc agcccgccct
     1861 tgagctcggg cacctgctcc ttcatgtggt aagcctcggg caggtcgaag tcgggcgcgg
     1921 agcgccgatc gcactgcgcc tgcgccacgg ccgagatctg cgagaggtca ggcgggttcg
     1981 gcacccactc gtggcccttg tagcgcagga agagccccgc cgtgatctcg gcacccgaac
     2041 aggcatagcc gacgaaggtc acggcgcggt gcgggttctc gatggcgatc tggagggcag
     2101 cacgcagctg ccaggagtag agcgagcggt ggcaggcctg gtcgagccag cgcgcgttct
     2161 cgtcgatgaa ggcgcgatcg ccgatctgct tccagggacc gacgcgggcg gggtaaccga
     2221 cgagggcggt atcgctgttc ttgcccttct gcggcttgcc gtagtcggcc gcgcgctctc
     2281 ttgagaagcg gacgggaacg tctgggttgc cttccccgga ggcaaagctg tcgcccatgc
     2341 cgacgatcag caggtcagag acacggacga tcgcgcgcgc gacctcctgc cccccgacct
     2401 cgacgcgcac atcggcgccg ccggggtagg gcacatcgaa gcgcagcggc tcagcgcacg
     2461 actgggttgc agatccgccc tcggcgtctc gcgtccgacc ggcgggctcg agcggtgtca
     2521 cgagccaggt gcactgaacg acgtcctcct cggcgccctt gagcgtgagc agcacggcat
     2581 gacggcgcgg attgagatag tcgggatcgg ccgggcactc atgacggttg gtgacggcgt
     2641 tccagcaggt attgcggaac atggtagccg cccagccgtc cgggtggcgc tcggcgagtg
     2701 cgcgctcggc ggaaaggatc ggcgtacgcg cctcgtcggg tttcagcgcg gcgaaggtcg
     2761 agcggtggac attggtatcg gccgggttcg tgaagaatcg gaacgggttg tcaacgcgcc
     2821 actcgatgcg cgcggcctgc gccatcgcgg gctcggagcc gatgaggccg gccaggacga
     2881 tcaatccgat tgctttgagt gccgtctctc gcaccaccat ccctttcgat cccgctggcg
     2941 ccgccgggct tgcgcggcaa gggttccgaa agcctctttt gccatgtttg cggcccaaat
     3001 gcggccccgc tgctctccgc ggccggttac gcacgcctgt cgtgatggcg ccacagggct
     3061 cggcttggtg gcgcaccgag aaaaacgtta acgtgcctga ccgattcgag gagagagcag
     3121 atgctccaca agccccgcgc cgatctgaag cccaacaccg ccgatttcga gcgcatggct
     3181 caagtgaagc cgacgggctt tcgcgagtat gacgcgcgct ggctcttccc gagcgagatc
     3241 aacctgatgg gcctcaacgc gatcggcctc ggcatcgcca cgctcgccgc cgagcgcggg
     3301 gtgccgaagc gcttcgtcgt cggccacgac ttccgctggt actcgggcgc cgtgaagcaa
     3361 gcactcatga cgggcctcct tgccggcggc gccgaggtgc acgacatcgg cctcgcgctc
     3421 tcaccgatgg cctacttcgc ccagttcgcc ctcgatgtgg agggtgtcgc catggtgacc
     3481 gcctcccaca acgacaacgg ctggaccggc atcaagatgg gcatccagcg cccgctgacc
     3541 ttcggtccgg acgagatgac ggcgctgaag tcgatcgtgc tcgagggccg cggccagacc
     3601 cgtgccgggg ggcgctacat cttcgttccc gacctcgccg accgctacgt gaagtcactc
     3661 accgaccggc cgaagctgaa gcgccgcctg aaggtggtcg ccgcctgcgg caacggcacg
     3721 gcgggcgctt gggcgccgaa ggtgctcgag gcgatcggat gcgaggtgat cccgctcgac
     3781 tgcgaactcg atcacacctt cccgcgctac aatcccaatc cggaagacat gaagatgctg
     3841 cacgccatgg cggaagccgt gctgcggcac aaggccgacg tcgggctcgg cttcgacggc
     3901 gatggcgacc gctgtggcgt cgtcgataac gagggccacg agatcttcgc cgacaaggtc
     3961 ggcgtgctgc tcgcccgcga tatttcggcc aagcaccccg gcgccaaatt cgtcgccgac
     4021 gtgaagtcga cgggcctctt catgaccgac cccgtgctca aggcgcaggg cgccgagacg
     4081 gtctactgga agaccgggca ttcctacatg aagcgctaca cgcacgagat caaggcgctg
     4141 gtcggtttcg agaaatcggg ccacttcttc ttccagccgc cgctcggccg cggctacgac
     4201 gacggcctcg tcgccgcgat cgccgtctgc gacatgctcg accgggccgg cacggcaacg
     4261 ctcgccgatc tcgtccgtgg cttgcccaag acctggcagt cgccgaccat gtcgccgcac
     4321 tgcggagacg aagtgaagta cggcgtcgtc gatcgcgtcg tgaagcactt cacgggcctc
     4381 aaggagcgcg gccagcgcgt cgccggccag ccggtgcgcg aattgatcac ggtgaatggc
     4441 atccgcatcg tgctcgagga cggcacgtgg gggctcgtcc gcgcctcgtc gaacaagccc
     4501 gaactggtga tcgtcgtcga gagcccagtg tccgaggcca agatgcgctc aattttcgct
     4561 gagatcgacg gcttcctctc ctcgaagtgc cccgaggtcg gcgcctacaa tcaaaagatc
     4621 tgagcggggg cgatatcgga cgatccgtcc tcgtgcgggc cggcgtcagc cttctccgcc
     4681 cgcactggcg gcgactgcat caggaacacc tgtcctgcgc cgcgaccgca tcaggtcgct
     4741 cgccgcctcg agcacctcga cgccggcgcc agggcggact gcgttctccg agagatgacg
     4801 gcggaacagg cgcccgccgg gctctccgtg gtagaggcca agaatgtggc gggtgatgct
     4861 cgctagtcgc ccgccggctg cgacatggca tgcggcgtag ggaacgagcg cctcgagaac
     4921 cgcctcgcgg tccggcggcg ccgcagtcgc gccatggatt cgacgatcga cgtcggccag
     4981 caggtagggc gtctggtagg cggcgcggcc gaccattacc ccatcgagat cctcgagatg
     5041 cggcaatgcc gcgccgagcg agcctatgcc gccgttgagc acgatcgtca gcccgggatg
     5101 tgcggccttc aatcgatgca cgcgcgagta gtcgagcggg ggaatttcgc ggttctcttt
     5161 gggcgagaga ccggcgagcc aggctttccg cgcatgcacg atgaaagtgg agcatccagc
     5221 ggcgctgacg gttgtgacaa accgatcgag atcagcctcg ctgtcctgat catcgatacc
     5281 gatgcggcac ttcaccgtga cgggaagccg gactgacgcg cgcattgccg cgacgcagtc
     5341 cgcgaccagc gtcggctcgg ccatcaggca ggccccgaaa cggccctctt gcacgcggtc
     5401 cgacgggcag ccgacgttga ggttgacctc atcgtagccg aaatcggccc cgatccgcgc
     5461 ggcgcgcgcg agcttgaggg gatcggaccc gccgagttgc agtgcaactg ggcgctcctc
     5521 tggagagtag gcgagcagcc gattgcgctt gccatgcaga atggcgtccg ccgtcaccat
     5581 ctcggtatag agcagcgcct cgcgcgtcag caggcgatgg aaataccggc agtgcctgtc
     5641 ggtccagtcc atcatgggtg ccacacagaa tctatgtgat tgagtttgca tcgtttttcg
     5701 agtatcttca gaacattagc ggtacgaaca atcgcgcaac attgcacacg tgtgcacgcc
     5761 ttttcgcacg gtttcgcacc ctcactgcac ccggattgca cacgtttgca cccatcccaa
     5821 gatcaactgc acacgaaaac cgagactcgt gtgcactcca gtggatcagc gtcatggcga
     5881 ccttcctcaa gctcccctcc ggccgctggc gcgcccaggt gcgccgcaag ggccgatctc
     5941 tatcacaaac cttcccgacc aaggcactgg ccgaggagtg ggcccgcgac caggagacct
     6001 ctctcgacaa gggtcaggcg ccgccgctcc cgaagccgcg gtcgccatcg atctcccacg
     6061 tccagccccg cgcctgcgag acgctcgcgt ctctcatcga cctgcatctg gccgatctcg
     6121 ccgagctcaa ccgcggcatc gggcgcagca aggagcacac cctcttccgg ctccgcgagg
     6181 agatcggcaa gacgcgcgtc gacgccatca cccgcgagtt cctggtcgac ttcgccaagc
     6241 gccggtccaa ggccggcgcc ggtcctgtca ccatcagcat cgacatctcg ttcatcggca
     6301 ccgtcatgca gcatgccgcg gccgtccacg gagtcagggt gccgatcgat gagttgcgcc
     6361 tcgggcgcat cgccctccat cgcctcgggc tcgtcgccaa gtcggccgag cgcgaccgga
     6421 ggccctcacc caaggagatc gagcagatca tcgagacggc caacgccaac ccgcgccaga
     6481 tcatcccgct cggccgcatc gtgccgttcg ccattgccac ggcgctccgc caggacgaga
     6541 tcagccgcgt gctgtgggag gactttaatc ccgagaaact gaccctcaag gtgcgccaga
     6601 ggaagcatcc gcgcctcaag accagcaacg accagactat cccgcttgtc gtcgataccg
     6661 gctacgatcc cgtcgccctc atcaacgtcc agggcgaacg caccggcaga cagaccggct
     6721 tcatctttcc ctatgacggg cgctcggtcg gcgccgcatt ccgacgtgtc tgccgcgatc
     6781 tcggcatcgn nnnnnnnnnn nnnnnnnnnn nnnnnncgaa ggtatcagcc gtctgttcga
     6841 ggccgactgg gacattccgc aggtggctat ggtctcaggc cacaaggact ggaagatgct
     6901 ccagcgctat acgcatctcc gcccgagctt catcgcccgc cgcggccgtc cgccggtcgg
     6961 gcgcgtggcg gcggagtgag ccgctcaatt tccttctcat tgccagcgcc taacgactgc
     7021 ctagccaacg cttctggctg atgaccgcga ctccaactgt ggacaaaagg gagagagtta
     7081 atcgttcctt gttcgttcga tccacccgct agcatatcct gctgtcctga aggagatgcg
     7141 ccgtgatcct gacggacaac gagaccaagg tcgatcttct caacaatgag gcgatcgcga
     7201 ccacgatcat aaagcttttg cgtgaccggc ccgagcagcc ggtcacgatc ggcgtgcacg
     7261 gtgactgggg cgctggcaag tccagcgtcc tggagatgat cgaggcgggg ctggacggcc
     7321 aggacaaagt tctctgcctg aagttcaacg gctggcggtt tcaaggcttc gaggatgcga
     7381 agatcgccct catcgaaggc attgtcaccg ggctcatcga aaagcggccc gccctcaccc
     7441 aggcggcgga agccgtcaag gaagtcttca agcgaataga ctggttgaag gtcgccaaga
     7501 aggctggcgg cctcgctttc accgccttca ccggtgttcc tacgccggat caattgagag
     7561 atctcgtcgg cgtgctcgaa ggaatggccg ccgatccggg aaagcttttg acgaaggaga
     7621 acctcgaagg ggcgatcgaa ggcgtgaagg acgtcatccg gcctggcggt gacacgacca
     7681 acgttcccaa ggagatcgcc gccttcagga aggccttcga tgggttgctc gacaaggccg
     7741 gcatagagca gctcgtcgtt ttgatcgacg acctcgatcg gtgtctcccg gacacggtga
     7801 tcgagacttt ggaagccgtt cggctattcg tcttcacgtc ccgcaccgcc ttcgtggttg
     7861 cggccgacga agcgatggtc gaatacgcgg tgcgcaagca ctttcccgac ctgcccgata
     7921 nnnnnnnnnn cgggccccca aacatacgcg cgaaactatc tcgaaaagct catccaggtt
     7981 ccgtttcgaa ttccgtccct cggcgaaacc gagacccgca tctacgtcac gctcctgctg
     8041 gtcggtatgg aactcggcga gaaagacccc gcctttgacg cgctcatcaa ggccgcgcgc
     8101 gagaagctca aaagaccctg gaaagtcagc gctctggatg cggatgcggt gaagcaggcg
     8161 ttgggcgaca agtcgactcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnntcgcac ggccgaggcg cgcggcttcg gtgtcgacgt gaagctgcca
     8341 gttctcgcca agctaatgct ggccgagcgc ttcctgccga gactattcga ccagatcgcc
     8401 gctagcgcgg cgaccgccgc agatggtcgg tgtccggatc tcgcggctct tgaagccgcg
     8461 cccggtcggt ctgaccgcga ggccggcgac agagtgagcg acgacgccac tgccggtgcg
     8521 gccaaaccca aagtgaagcc gggcctcgcc aaatcatccg agccatccgc gctcggtgag
     8581 tggtcttcgt ctcctgccgt ccgagcatgg gcttccgttc ccgtcgctct cggcggtgag
     8641 gacttgcgcc cttatctgtt cgtggccaaa gatcggaaag actatttcgg cacaacgtcc
     8701 gcgttgggcc atctcggcgc ggtcgtcgaa caacttttcg gcggaaaatt cgctgttcag
     8761 gcccttgagg ccgacctcaa gcgcctcgct ccacccgaag ccgccgaggt gttcgaggcc
     8821 gtgcggaccc gcatcgtcgg tggaggcgaa tttgaaacgg agcctccggg gattcctgga
     8881 ctttccgtgc tggtcaaagc gcacccttcc cttcaaggca atctgctcga ttttctggaa
     8941 gctttacctc gctcgaaact gggcccctgg gtgtgcagcg gttgggatag cgcccttccg
     9001 gatcagaacc actccaatcg cttcaccaaa ttgctcgaag cctggagcaa ggagggcggc
     9061 aaactcctga aggcagcggc cacgggcgtc cacaaaactc gaggaggccg ctgatgggga
     9121 catccagcgc cttcggcgga cagagcggcg gcactccgct cgtcccgagc tggcttgagg
     9181 acggcggctc gcctcccgcc tcgcctcccg tcgccccccc tttaccgcct ccgccggcat
     9241 cccagcaacc cggtgccgct cctcccgtga caccgccgtc ccttccaccg atcccgcctg
     9301 gcggtgaagc ttcgcgcttc tcgtcggcgc gttcgaactt ctcgcggttt gcccggtcgg
     9361 gtggcaatga ccggaagagc ttgggtcggg cggtctccca ttacgtgaga tcgtccaatg
     9421 ggagtgcgcg aacggcctcc acgcggatgg gatcgtctcg cggcgcgggc agccggctga
     9481 tcggcttctt ggctgacgcc gtcgagcgag ggcaaacgga ggcccttcgc gctctcgacc
     9541 tcgtggctct ggctggccgc cccatcgaag aaatcttcct tggattggtc gattacatct
     9601 gtcccgacgg cggcccgatc gatgaaggca tcgcccgcga ggccttcata gagacgatca
     9661 ccgaccttgc cggtgcaggc atcaccgaca tgaacgactt taccgccgct cagatgcaga
     9721 cggtattcga acttttcgcg acgaacgcga ttgaagcgcg gctctgcaac gatattggcg
     9781 ccaagacgat tatcctgccg tccgatgcac gggaggccat gcgcgtccag cagcagctgc
     9841 acgacttcat ccgccgcgct gtagcggatg cgctgtcgag cgccagcgcc attcggggtg
     9901 cgctttcccc cgctcgtgcc ctgcagttcg tagatggaat ctatcagcag gctttcggca
     9961 tcctgcagat catgggcgag gcggaggccg atacgccatg aatcgtcaca tcctcttagg
    10021 tcacttcggc gctgcggatc gccctcgctt cactcccggt gcagaagaag tggcgacccg
    10081 catcgacttg ctgaacggcc aagactcgct cgatcacggg atcggccgtg cgctcgggga
    10141 cttatcccgt ctcggtgtcc gcccctcgga actggggatc gacgttctga tactcgcagc
    10201 gcacgtccac gcggccgaca cgcggatttc tcgcgggatc gttgctcagg acggttggac
    10261 tcgcgaaatc cggctgcttg tccccgtctc tgaccctgac cgatggcgcg ccgccgcgcc
    10321 ggtttttacg cgtatgctca agtttctgac gggagaccgc tgggaactga tgtttcgagc
    10381 gaggccccgc cggcatcgga cgctcgctcc accgagcccc accccgctac tgccagcgcc
    10441 attcgaccac ctcgcactgt tttcgggcgg attggacagc ttaatcgctg cgatcgactg
    10501 tttgcaagca ggcaggaccc ccctcctcat cagccacgcc ggcgaagggg ccacgagtga
    10561 cgcgcagaac accgtcttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnacggctg cgcttatgga tgaatttccc cgacgggatg gtcgacgaaa cgtcctccga
    10681 ggacacgacg aggggccggt cctttctctt cttctccctc ggcgtgtttg ccggtaccgg
    10741 gctttcgcat ccattcactc tgaatgttcc tgagaatggc ctcatcgccg tcaatgtccc
    10801 gctcgaccct ctccgattgg gggcgctcag cacccgcacc acacatcctt tctacatcgc
    10861 ccgttggaat gaggcgcttt cggcgctcgg aatgaatggt cagatcgtca acccttattg
    10921 gaacaagacc aaaggcgaca tgaccgcaga gtgccgccac agcgccttgc tcaagaaact
    10981 tgtggcttcg tctctatcct gcgcatctcc gaccaagggc cgctggcagg ggcacggcac
    11041 ccagcactgc ggatattgcg tcccctgcat cattcgccgg gccgcgctcc ttcgcggcct
    11101 gggtcccggt aacgatccca ccacctacac tctcgcagat ctgacggcgt cttccctcaa
    11161 cacggagcaa tccgaaggcg ttcagattcg ctcttttcag cttgccgtcg aacgcattcg
    11221 ggcccggccg agcttggctc cgttgctcat tcacaaaccc gggccgctgt ttgacgaaac
    11281 cacacaagcc caggccgagc tcgccgaggt gtaccgccgc ggtctcgacg aagtcgctgc
    11341 cctcctcgcc ggggtcagga cggcacccga atgagcgatc ctctgcaggc ccgcggtgtg
    11401 gattttcatt gtcacctcga cctcttccct gatttcgagg ctgccatcgc ccgtgccgag
    11461 cgcgcgcagg tttacacgct ctcggttacg acgaccccga aagcgtggaa gcgaaatctc
    11521 gaactgacga aggacacgcg ttttgtccgc gcagcacttg gcctccatcc tcagctggca
    11581 gcggagcgcg ccgatgaact gccgctatgg gaagcgctcc tggatcaaac gcgctacgtg
    11641 ggggaggttg gtctcgatgc tggtccaaaa tattaccgat cgctggatag gcagaagctg
    11701 atattcggcg ccattctgcg gcgctgcgcc ggtaccggag gaaagatcct ctcggtgcac
    11761 agtatacgct cagttcccat tgtcctggac atgatcgctc aatacctgcc ccccggccgt
    11821 ggcaaagtcg ttctgcactg gtttaccgga accaaagctg aagccgctcg cgcagccact
    11881 atgggctgtt atttctcggt caacgccgcc atggcacgat ccgagcgcgg tagggcaatt
    11941 gtctcctcga taccaccgca tcggatactc accgagacag atggaccatt cacacagtct
    12001 gcgggaaggc ccgcagagcc agctgacgct ttgcaaacgg tggaatcaat tgcatccgtt
    12061 cggcatctcc caattaagga cgccacaaac ttggttttag ataacctgcg agatctcctg
    12121 acggtctagc ctgcacttct cacagccgcg ctgacgctgg tgtcgtttgt cgattgagtg
    12181 ggagccgccg cggtcctggg gctcccacgg gcgagcccta tggtcgccgc cggtcgggca
    12241 gcatgggcag gctcggcgat gatcctctgc tgttgatccc agggctcagg ctcggcttgg
    12301 ttgccggcgc ctcatagtcg tcgcgccgcg agcatgaaga cttcgcgatc cgggcaggcg
    12361 gttgcgaagg cgagtttcac gcgtctcacg ctgatcgtga cgcgggcgcc gatcttgaga
    12421 agcttcagcc tgatggtgcc gcaggtcgcc gctgcgagct tggtgccggc cagaccgatg
    12481 cgcctgaccg cgcacatgag cacgtaggcg aaggatgcga gccacagccg tagttggttc
    12541 gcccgcatgg tcggcgccgg cgtccggtcg gcgaagaggt cgccctggca ctccttgagc
    12601 cggttttcca tctcgccgcg ctggcagtac acatcctcgt agaggaagcg cgccggcccg
    12661 aaggccgcat ggatgttcgt tacgatgaag cgcggattgg cggcgccgtt ggtccattcc
    12721 gccttgccga tgacgcgccg cctggcactc cagctcatgc gggtccggta ctcgaagtcg
    12781 gtgaagacgc gcgccgcctg ccccgattgc ttcgcccgcc gtctggccct gcgcatctcg
    12841 ggtgcgagct tgcgaacgag acgggtattg ccagcgagtc ccagcacata ctgcacgtcg
    12901 ttggcctcgc accacgtcat caggtcgtcg cggcagaagc cagagtcagc gcggatgacg
    12961 attgtcgtcg caggccaacg gatgcggatc tgggcgacga tgcgggcaac ctcggcaacg
    13021 ctgccgtgag cggcgtccag gctcgatgac ctgagcttcg ccgcaagcag gtgccggccg
    13081 cagaagacgt aaagcggcaa gtagcagtag cagtcgtagt agccgtggaa gtgccggccc
    13141 tcctgctcgc cgtacacggg atcgtcggta gcatcgagat caataacgat gcgcgatggc
    13201 ggcctgtcct gggcgtcgag gaagagatcg acaatcagcc gctcgattgc ctccgcgtca
    13261 tggtcgagct tgtggtgccg gtcactgccg atcttgcccg catgctcgag ccgattgagc
    13321 gtcgacttgc cggcgagcct gtcaccgggc ttttcgagca cggcggcgag tgcgggatcg
    13381 tcgcgcagcg catcgtggtc gatcaggtcc tcgtgcccga gcgcgatgcc gaaaatgcgc
    13441 tggcgcagca gatcgggaac cgagtggcgg acggcgtcgg caacgcgcag atcggtgaag
    13501 catctcgcga ggcggcggcc gagtccggtg acccggtcgg ccgcagcgag cagaacgacg
    13561 ccaccgttgg acgtgatggc gccgccgtcg aaagcgccca caagtttgcg gccgcccgac
    13621 cttcccagat caaactcgcc ctggctacac tgtgtcggca tcggggatga tctcctgctg
    13681 ggcgcaaggc tttgtcgcag aagaactttc gctgagtcgg agccccgatg cgcatcacat
    13741 gcgtgagaag ttcaggctag gtttgatgca cttcagtatc ttggcgcgat ctctgcagta
    13801 caccgcgccg ccgccagcga gttgggcgtt ctaggccatc gggacgcacc ggtcgactgg
    13861 cgtgacgaag gccacactgt tggcatcgct cccagacggg ctagtcgcct ttcccgcgct
    13921 ccaccctccc ccgcgccagc caggccagta cgtcggcgcc atggtaggtc accctcccgc
    13981 gcctgaaggt gtgcccgtcc ggcttgacgt acggtggtcc gccgcgcccc tgggatcgcc
    14041 atcgctccag ggtccggtgg ctgatcccga tcacccgagc cagttgcttg cccgtgtaga
    14101 gccgatccgg gtccagctcg cgaacggccg gcaacggacg cgcttccaac gtgtgatcga
    14161 cagttgtgcg cgccgtggac gcgtctcggc cggaaacccc ggcgtcggac ggttgatcga
    14221 gctcagctgt gcagtcaccg gactgcaact cggcgcgctg acggtagtgc ggcttgctga
    14281 agtagttact catacactgt tcctttcagg ctggcagatt tgaacgcaaa gcttctcacg
    14341 cctcgaggcg tggcggatcg gatgacgcgc ttgatcgcgc gatgagtgca gtttcgatag
    14401 ctgacttgac ggcgcgtagc tcgccgatcg gaccccagac cacagcgtcc tggtcatgca
    14461 gtgcgaggct gatcagctgg agccgaccga tgcggaacga cgcgacaatc gccgccgcgc
    14521 cgaagctcca gatcccgatc agctcatgat cgtacagctc gcgggtgaac acggccgcag
    14581 tggcggcgag gccccctgct ccgatcacgg cgaacggcaa ccagggtcgg cggagcttca
    14641 catgctcgac attggcaagc gtgaacgtgg acgaacgcga cgtcacgtgc gtggtcgtcg
    14701 ccagaaaccg ccgggttcgg cagatgatgt tgtcggacat atcgatctcc ttcaaagcca
    14761 ccggaaccag ggacagggtc ccgacgtcgt gacgaaaccc ttgagcccgg cgtggatgca
    14821 gggtctcggg gcgtaactga cggcgagatg cggggtgccg gcgacggcga cggcgacgaa
    14881 gatcgccgcc caagcgatga tccacccccg cgggcggagc gcccaggcga cccggccgag
    14941 catcgatgcc cacggcgacg ggcgccgctc agccgtcttg atggcctgga tggcaagcac
    15001 cgcgccgaca acggcaaaca tcccactagt gaacgtgccg taggcccgga caacatccgg
    15061 tgccgttgcc gacggcgcca tccaccaggc aatgagaagc gcaccggctg cggcgaaggc
    15121 gctgagattg gacgaaggtt tctgagacat gtccctactc ctgttggcat gtgcgaccgg
    15181 gatcagcggc ggccatcgcc gcgcgaaacc cggacgatgt ggtcgaaggc ggcgcgatca
    15241 gcttcagcgt gccgacgtag atcgcgtgct cgaagcggac ggacgtctgg ccgagtgcaa
    15301 gttgtgcgag gcagcggcac cgcgccggac cgaccgatgc cgtctccgcg ttgcgcaggc
    15361 cgcgcttcgc atcgcgcaac agctggatca catcggcaac cggctccggc agttgcttga
    15421 gcgcgcgccg ggcgacctcg tcctgtagcg tggccgaggc gccgcgatcc gagccgaggc
    15481 tcacgcccgc gatgcaggct ggaatgtgct cccgttcggc caggcgctca gaaatcgggc
    15541 cagccaggag ccaggcgatc gcgccatagg cgaccagccc tgccaacgcc tctttnnnnn
    15601 nnnnnnnnnn nnnnnnncgc cctcccggaa tggtcgagag accgcacgtc ggagcatttc
    15661 cgggcggcgc cgcgaggcgc cgtccagact tcgatcactt catcgatcct ggcccggatc
    15721 gcacggtgat cactatcgtg ggtgagaaag acgaggaggc cgactacggc caagccggcg
    15781 agcaaaccga gcacgacata gtgcaagacg ttgatcacaa ccccggcaat ggcccaagcc
    15841 cactggtggc gcgttcccag tctcatggca cggcccttcc attcgggggg ccgacatcgt
    15901 cgtcgtcgcc atcgtcatca aggtcgtcgt cgcggttctg acgttgtgcg tgtacgaaag
    15961 cgtacatcca gagcagcggc gacccgcctt cgacgagtaa tgcgatcaac agattgtgcc
    16021 aggttcccgc gaacttcgag ggctcgaatg ccgccacgag caccggagaa gccgccatag
    16081 gcggcagcgc gtccggcatg cgcggcaggt ttgcaagctc gcgatcgagt cggtcagaat
    16141 gccccctgac gacacgacgc gcgtcctcga tggcaccctt gtcgatcgtg tcggacgaag
    16201 cgagcgtacg aagctgatcc gacagcgagc ggacgcctga caaaggcgcg gcctgagcca
    16261 gctcgcgaag tcgacctccg gccctgtcat agatggcgcc gacggccgca gacccctctt
    16321 tgcgccactt ctttgggtcg gaaatcagtc gttcgcagtc atcggcgacc gaggcaagat
    16381 cacgcacggc ggcaatacgc tccgcgccac cgcgcgccaa ctgatcctcc gccagcccgg
    16441 catagcgccg ggcgtcctca tagagcgtcg ttactggctg cggacgacct gtgacggatt
    16501 tcttgccact ctcacggcca cgggtggctt cgccgtgcgc gcgcgcctgc aggtcaacgc
    16561 cgatcgcctt gatcgacttc tccagcgctg ttcgcgcaag cacgacctca tcgagttgag
    16621 aggcggcttg tcggatgagc tggcacttct caagtgcgtc agtcttgcgg acttccggcg
    16681 ccgacagcgc caaaaatgaa aggaccacga tggttggcgt cagaacagcg aacgtcagtg
    16741 ccgaggccgc ccccgccaac accgatccct gtgccgcaag cggcgctccg cgcacgatcg
    16801 cgaggcggct gacgctgtag ccgccgaagg tgatcgccag ggtggccagg acccgcttca
    16861 ggttggcggc aaagcctcca gcgtccggct cgatcagctc gaatgacgcc agtaggacga
    16921 ggccaccggt caacgtccag accatcatct gaacgaatga cagggtgcgg tcgcggcgtt
    16981 ttcgcctcgt ctcgtatggt gatatctgag tcttgggtgg tcgtggcatg gtaggtaggt
    17041 ctccatgagg ttgattagcc ccccatggcg acgctacgcc ggtcgctagt gacgatgttc
    17101 aaacccgagt cttgaacttt ttggccggaa tcgccctcaa tgttcgaacc tgcgattcga
    17161 actcctcgga gatggactga tgtccagcat ggaaccggac cccgcgttgg ccgctgcact
    17221 atgtaagctt atcacgacca tgcgaactcc aaagcgaaag tggtccgtcg cggcgatcga
    17281 acgcgcgttg atccgcaatt atcaggacta cggcgcaccg gcacgtggac tgtctgagcc
    17341 cgtgatcaca gacgccaaag aggggcgccc catcagctgg aagactaagg acatcctgcg
    17401 caagttcatt cacgtcaatg cccctggatt gctgacgcca ctgatcccgc cggttgacct
    17461 cgatctcggc actttcttcg gcaccggcac cagcgagatg acaagagcag gacgtcggat
    17521 tgtgggtacc tatcagacct acgcccgctc gacctcgatg ccggcatacc tgcgccgtgg
    17581 acggatgcgg ttcgactacg tcgacgatga cgacaagcag cgcatcgctg tcgttgaaga
    17641 gcaacttaga cccgcaatcg agggtcgcga agaatggccc cttcgttggg acggcttcgc
    17701 cgctcctcgc ggccggagct acttcgttgt catgcgtatc cccgaagacg cttatgggcc
    17761 tggtacagcc agcctcggaa tcatcgatgt tgctacttcg ggccccgggg tcgtcagaga
    17821 ggcgattttt catggattac aatacgatgg cgagaaacca ggttacctga aattcacctg
    17881 ccttttgatg agaacgcagg ccgattgcat tatggattat gtcaaattag tagatttgac
    17941 agataaagaa caattcatta tagatgcatt atgtctcgca gaggaaatag agaaactacg
    18001 aacggcacgc catcggtaaa aacttacaag accatgttcg gggagatcgc cgggccggtc
    18061 gaattctgga ctcgggccga cttcaccgaa gcagaactgg ccaacgattc acgattgcgt
    18121 ggctatggca accgtctcct cggcagcgac tacaatcact gggaacaatt tcccaagttt
    18181 tcaccggagg agatcgcgcg gcgtcgtctg gagatggtgc aagcggcgat gcgggcgggc
    18241 cacaccgagt ttgccatgat gatcctgaat ggccctgaca agctaccgca gttccgcgcc
    18301 cgttggatac ggggtctgtg gcctgttccc ggcggaccgt ggggtgcgcc caagttctgg
    18361 gagaaatacc tgtagtcggg ttcagtgcgg tcgcagacga cccgtgtttg agaagtcagc
    18421 ggtttgccct gctcggctta ccacttcatc caccgcctgc agacgccgcg cgatcgcgga
    18481 ggccccctgc tcgtccgaca cgtcggcgag caggacgaac acctcgccat gctggagcca
    18541 gacctgccag gcctcgcggt acgtcgtcgt gagcagtcgc tcggcgtggc gctcggcccg
    18601 gtcctcgtgc cggccatcgc gatgcggcac agccgtgaag cggagagccg ggccacgcgg
    18661 ataagtctgc ccgccgtgcg tgaccacgcg gcgatgaaat ctcacctcca gggtcctgtc
    18721 cgtgacttgc cgcgcccaga agcgatggcg aaaagcgagc tgaccgatca tgcgcgccac
    18781 agcgtaggcg ccgatcgcgc cgagtgctgt caggagaacc ccgtnnnnnn nnnnnnnnnt
    18841 gcgccaaatt gcgacggtcg aagcaagcgc gatcatgcag gcgagtgctc cggtcgtgat
    18901 cgcttgcagg ctctccatgg cccgcaccat ctctggcgtc tcgtagccca cgatcacgcg
    18961 cacgccatcg ccgtcgggcc ggctctcgat ccgcggctca ggaaaccgca tcaaagtctc
    19021 ccgcgaagag cgcgtgcgtg tcgtacttgg cgcgggagag gatgatcggg tcgtatcccg
    19081 accagatcac gagctggcgc aggtacgcat cgtcacggcc gaacaggcgc gcgatctcct
    19141 ccgccgtcag caatggttgc gtcgtcgtct gccaggagag cccgcgccgg tccgtgcgcg
    19201 tctggtcctg atcgctgacc tcgcccttat tgcgcacgat caggctcgtg gtgccgagcc
    19261 ggcgcgagac gtactcgagc gtgaactggt cggaattggc gaagcactgg atcacgccgg
    19321 cgttgccgag gaaggtctgc caacgctcgg cgtagagtga tttgagctgc cccaggtcct
    19381 gcaggatcgg ccacagcttg acgccgaagc cggcgatctg gccggccgcg tcctcgatgg
    19441 cgcgcatctt gccgagcgtc gcgaactcgt cgaggcacag gacgaccgga agctcgggct
    19501 tcgccgactc ggcctcgaag gccgcgagcg ccaggttgac gaacagacgg agccagcgcg
    19561 cgcaggtgcc gaggcgcatc gccggcaggc agagatagag cgtgacggcc tcccgcttca
    19621 ggtctaccag atcgatgtcg tggccggcga gacagtcgtg gattgccgga tagctcagga
    19681 accttgcgtg acgccgtgcc gtcgagagta cgctcccccg ctcgtcatcg ggtttctcga
    19741 agaagtcaac cgacgcgtcg atgatcatgc cggacgcggc atcgttcgcc tccatctcgg
    19801 cgcgcaggcg gtcggccgcc ttgccttttt tgccggcaat ggccgtctcg cggaacgcca
    19861 ggaggtcgcg caccgcgccg agatgccggc ggccctcgta gagcgggcat gttgccacgt
    19921 gcaggatcag accctcgatc agattacgcg ccgactcctc ccagtgggga tccctctggt
    19981 tttctgcggg gatcacgatc gcatcggcaa tcaggccggc gtcctcgatc agtgtcgggc
    20041 tctccgtaac caggatcgtc atgggattga agcgggcgcg caggttcgcg gcgaagccac
    20101 tcgtgacctt gaacgggtcc agaacgacga cgcgatgacc aagcttctcg acgcgcgccc
    20161 gcgccgtcat ggtcgcgagc tcgcccttcg ggtcgatcac cagcatggcg ccgcgatagg
    20221 tgagcaacgt cggcacgatc accgagcggc ccttgcccga gcgcgaaccg gcgaccgtca
    20281 ccatgtgccg atcgtcgcca atgccgattg gcatgccgtc gacagcgacg cgctcgcgac
    20341 gctcgccgaa gtcgcgatcc tcgaaatctg cgccgaccag cccgagaaag atctttgccg
    20401 agtcgtggcg gagcgcctgg cttgccgcga tggcggccgg cggcatgatg gccgccatag
    20461 gcatcgactg ctcttccagt gtcagctcac gccccgcgcg ctccagcacg ccgcgcggga
    20521 tgccggtcag gatgtcggtc gcatcgcggc tctttcnnnn nnnnnnnnnn naagagattt
    20581 cggcgacgtc atgggctggc tcgctcgctg gggaatcgag cctgaaagat cggctacgcg
    20641 tccccgccgg tccaaccgac acgagcgcgc cggaatgcgc gagatcaccg gttgtcgtgg
    20701 accgcaggca aatcatgccc ggccatcgga cggtccatgt ctcgggctcg atcttcgccg
    20761 ctctctccgt cgggtgacgt tgctggcaac cggtcatctc cagccatgat cgatgggctt
    20821 atcgcatcgc cgcccgaccg cgaaggcgct ccgtcaacgc ctgcgaaaca caagctcgcc
    20881 taaggcgcag gcgccaaggc tcgctagcat ttcgtctcgt tgacggtcgg gcccatggcg
    20941 acgcgatgcc aggggcccat caggctggaa tgaaccggcc agcaaaccca aggagagaac
    21001 atgcctacga agaacgaacc caagaaccgc ccgcaattcc gggtctgttt tgcccgcaag
    21061 acaggcgtcg acgacaaggg cggcgacgtc cttggatcgg cgcagcagat cggatcggtg
    21121 tggtcccgta gcgggggacg cggctccatc ctgcgcttcg atcacatccc tatcgagctg
    21181 acccagcaca acggcgtgct gttcctgttc ccgaccgagt ccgacaacga cgagcggaag
    21241 ggttactgat gcagacgctc cttcccttca tgactcacac cacgccggcg cggctgccct
    21301 ccggggcagg cgcccggcac cgggcccagc ccgggcgcgc gccgcacgag ttctatccga
    21361 cgccacccgc ggcgatccga gccctcctcg cggccgagcg cttcgacggc tctatctggg
    21421 aacccgcctg cggcgacggt gccatctcca aggaactgat cgcgcacggc tacgacgtga
    21481 cctcgaccga tctcgtcgac catggctacg ggcgtcacgg cgtcgacttt ctcgcgaccg
    21541 tgttcccgcg cgggcgcaac atcgtgacca acccacccta cggatcgggc ctcgccgacc
    21601 gcttcctgcg gcacgcgctg agcctgaccc gcatcaccgg cgggcgcgtg gccatgctcc
    21661 tcaacatcac atcgctctgc tcaccgaccc ggcacgactc cttcatccgg cggccgccgt
    21721 cggtgctcta cgccctcgac gactgcgtct gctggccgtc cggcaagccc gcgctcgcca
    21781 cgcggcatac cctcaaccac cgctacgtct gggcggtgtg ggagcccgag accgtaccgg
    21841 tgaccacaac tgccttccgg tggctcgcga cggcgccgtt caccgacgac gccatccgca
    21901 ccgccagacg ttcgacccgc tacacctcaa cccgaaccaa ggagactgca tgaaccacta
    21961 cattgcccgt ttcagagtct attgcggaaa acacggcttc gatcagatcg cattgttcgc
    22021 gtgccgcgat gcaccggagc actacctgcg cgcgattatg agaggctggg tgccaggcgc
    22081 gaccgctcca ccttccggcg atccgcactc ggcggcggtc gtcgcggacg gtatcgtcat
    22141 cgccagtacc ttccagatca aggcgatcaa ccgtgacacc tgggagatgc tgacgacgct
    22201 agttcccgag cgggggccgc gtgtcgaatg tttcgatgcg tccctgccga aacggcgcta
    22261 cgcgatcgag tacaggaccg aagggttgca cgagatcgcg gtcgaggcca cgagcgccga
    22321 tcatgctgcg atcctcgcct acatcgctca tgactgccgc aaaccggcgc cgacggagat
    22381 cgacgaggag gccgaggtca ccaaggtgcg cgaggtgccc catgcctgat cgtcttgccg
    22441 taaggcgcgc cgcacgggcg ctccgatcct attcgagtgc gacgacaccc gacttgtcgt
    22501 caggcgaagc cgcaatccac ctgctcgccg atctgcgtca cctctgcgat gtccaccacc
    22561 tcgatttcgg tgcggtagat cgcttggctc atcgccgcta cctcgctgac cttgccatcg
    22621 agaatgcgag gacgccatga tcgtcatgcc tcctgcccac tcgttccggt gcgcgcgccg
    22681 atggacgacc ttcgagcgcc tcttcgcgcc gatcgcccgg gacgacgact ccctgctctg
    22741 ggacgcgcgt gagatcgcct ttgccgtcga ggacccgcgc cactggtgga ccgtcgtcga
    22801 gggcgaccgg ggcggtctct acaccgttgc cggctggcac ctcgtcaacc gcatcggcca
    22861 cctctgctgt cagcatgcct ggggtggccg gccgggcgat catgcggcct accgctactg
    22921 atcgtcatcg ccccgcctga acgcgatgcg ctccaggagg tgtgaccacc tcggttctgc
    22981 ggcatgggct ttcggcgccg tgctgaggtc ccactgtgtg tcgtgccggg ccggtctcac
    23041 atccagcctc accgtgcacc ctatcgcggg gcggaccgtc gcgccgtcag cgctcagtca
    23101 acggcttcgt gacacaacct cggctagggc gcttacgcgg cccaagcctc gctagcatcc
    23161 cggtccgttg actgccggtc ctcgcgcccc gcgaccagcg ggtgcatcgg ctgg
//
LOCUS       DAORNH010000031        22925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1726_length_22925_cov_2.834150, whole genome shotgun sequence.
ACCESSION   DAORNH010000031 DAORNH010000000
VERSION     DAORNH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22925
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_22925_cov_2.834150"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PK264_05275"
     CDS             <1..838
                     /locus_tag="PK264_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HRD75337.1"
                     /translation="KVRAGRSLKPEVWPGGARAAVALSFDSDHETSTLRFGQTSPGKL
                     SMGEYGARVGVPRILALLQAYDIRASFFVPAVVALLHPDEQRRVIAAGHEIGIHSWIH
                     EFNSELPAAAERELQMRAADTLERVCGVRPVGIRTPSWDFSAATLAITREMGLLYDSS
                     LMADDDPYELIEDGRPTGIVELPVEWIRDDAPYLAMDRSGTARPYTPPLAVLDIFKAE
                     FDRAYADGGLFLLTMHPHIIGHSSRIAILEALIRHIRQQRGIWFARHDEVARYCKAAA
                     GL"
     gene            872..1831
                     /locus_tag="PK264_05280"
     CDS             872..1831
                     /locus_tag="PK264_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRD75338.1"
                     /translation="MSNKPLLLLQVADAAVYKAALDRKGVGQAVDIETFQRGTPPPDS
                     ALARADILLAFDIPAGMLTKMPKLRFIQSQTVGVDGWLARGDLRPDLPLAAARGTHRV
                     QMPENILGALFHATKPYAAISADQKERKWMRRASDTLAGKTLGILGLGAIGAELARKA
                     AALELTVIGTKRTVEPLPHVAKVYAPDSIDEVLGRSHFVVLLLPATEATVNLMNATRL
                     AGMRKDAWLLNFARGALVVDEDLIAAVKARTIAGAILDVFRTEPLPEQHPFWTTPGIH
                     VLPHIGGMHPDRDQMVAALFADNVTRVLNGETPSELVDRKRGY"
     gene            complement(1842..3089)
                     /locus_tag="PK264_05285"
     CDS             complement(1842..3089)
                     /locus_tag="PK264_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD75339.1"
                     /translation="MSTPQRNDRPRGRSFFFNPGPTNIPDSVLGAMHRPTMDFFAPEF
                     LEIFNSVHASVKRVLKTKQHMLMYASNGHGAWEAGLANLLKSGEKVLVLESGRFSASW
                     GDMATSLGLEVETLAADWRIGVKPEALTERLRKDGKAEIKAILVVHNETSTGVATPVG
                     DMRKAIDAARHPALLLVDTISSLGSLDFRMDDWGVDIAVGGSQKGLMMVTGLAFTGIS
                     EKAMAQWKAEAGKGGMRRSYWDWSEMLTHAPQRFPGTTPVHLFFGLEQSLKLMEEEGL
                     DAVFRRHSRFAKAVRAAVSHWGEGKRSKATLNLKGLSGSVGAIELLCADPARASDSVS
                     AIMLPDGIDGNLMRKIAQDRFNLSLGGGLGPLAGKAFRIGHMGDLNEPMLLGALATVE
                     LAMRAAKVPHKPGAVNAAIDALG"
     gene            complement(3157..4350)
                     /locus_tag="PK264_05290"
     CDS             complement(3157..4350)
                     /locus_tag="PK264_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LanC-like protein"
                     /protein_id="HRD75340.1"
                     /translation="MIDPARRRPLDQAPWTVAEAEVEIRAIIADAAANFTPDRLWPAH
                     PSDDGVSDGTAILYFGAAGVVWALDHLCERGYGSSGIDLAGLLPRLLERSDADYIEFA
                     APFPGYERHGSLLLGALGTALVAMRIVPDPGTADRIFELCRANSALPVRELMWGLPGS
                     MLAAVVMGATTGEPRWRALFEEQAALVLADLRDSALGPMWSQDLYGSVKPWLGAVHGF
                     AGNAVPLLRGWHWLTRAQQARVEAAVTIVLQRTAVASDDGANWPGVIGEPGDLCQHCH
                     GAPGMVTTFADVPFSSPELEALMVAGGHFIWSVGPLAKGSNLCHGTVGNGYALLKLHR
                     RTGDAVWLDRARAFAMTAIAQCREARRALGRGRYSLWTGDLGLACYLADCITADPRFP
                     TIDEW"
     gene            complement(4347..5258)
                     /gene="ppk2"
                     /locus_tag="PK264_05295"
     CDS             complement(4347..5258)
                     /gene="ppk2"
                     /locus_tag="PK264_05295"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HRD75341.1"
                     /translation="MADTPRKTRKAAPSSPLDAFDIMEPKLDKAIDEAALGSGGFPYD
                     TKLDRDTYERDLETLQIELLKLQTAVQKDRRRLVMVFEGRDAAGKGSTIKRISAHLNP
                     RHARVVALSKPTDAERGQWYFQRYIQHMPTAGDVVMFDRSWYNRGGVERVMGFCTPDQ
                     LADFLREAPHFEEMLVRDGVHLFKFYLDIGREMQLKRFHRRQHDPLRQWKITDIDRAA
                     IGKWDAYTAAKEEIFRFTHTQASPWTVIRANDKRRTRLEAMRVVLAALDYAGKDKKAV
                     GKPDPHIVGSGPAFFYPATGQKPDPDK"
     gene            complement(5286..5888)
                     /locus_tag="PK264_05300"
     CDS             complement(5286..5888)
                     /locus_tag="PK264_05300"
                     /inference="COORDINATES: protein motif:HMM:NF023939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3726 domain-containing protein"
                     /protein_id="HRD75342.1"
                     /translation="MRVSFNEIEGTVLKAARGAGYPWGLAEEASRAARFLAASGLPWL
                     ETAAAAFSEAAVRAIGGDNWQLNPIAAAAWYADFGGAKGGAFSGARMLAPVWFAGVLA
                     VGTGRIGEAMAISWIGHDRPAKLVIWRGALVSAEGALAATEATTFECRVVGPAVAAVT
                     DCHVDERRWAALQALEKNTYVPASDHSRIAGAGAGLVDND"
     assembly_gap    5989..6013
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6014..>6367)
                     /locus_tag="PK264_05305"
     CDS             complement(<6014..>6367)
                     /locus_tag="PK264_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75343.1"
                     /translation="RELDFNSPAENARFWYVSEEKLEPRLGDRANEPGAEREQPLAIA
                     RDVTLLRRALEAPHAAMPVAGFVSAYPELRHTVRRVQTTSRHPYAEIEGNLIAAGMRP
                     IDLLRCKLAFFGATRF"
     assembly_gap    6368..6377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6378..7605)
                     /locus_tag="PK264_05310"
     CDS             complement(<6378..7605)
                     /locus_tag="PK264_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75344.1"
                     /translation="MGTARELHHATVPLRPPGVVMRLARMGASHQTRLSFLRALLRRA
                     ARERWRFDRPHFDICREGFGTAVYRARLPSGAVSLVCFSTLLAPEKRTDRVIAEAWDT
                     SYALYDGEPDEAAIARLRENVPRQEAGRYSVRELVLARANRSVRLFDTVVDALAAGRQ
                     PDRRTIEAVGYLMRTTAVYGNGKFGIADRDVVLRHPELAGPFRAEMLAVWLIRTFTLD
                     LVEHVAQARGGARAVAIEPAVRRLFGIGNATGLGMAPFLVKHPTLIGRWIGARETALA
                     RVRSRAEATPDQLARFSDALARARTALPVWQTGDPVQAPRIEGVARDLATLADALVEP
                     RRALDGVGGLWDRIYRYAEARFAMEAQEMVVSLLLEPHGDLVDDLAETMAVDETSEHA
                     IDGLMTCGTLAGLIDRH"
     gene            complement(7612..8718)
                     /locus_tag="PK264_05315"
     CDS             complement(7612..8718)
                     /locus_tag="PK264_05315"
                     /inference="COORDINATES: protein motif:HMM:NF015457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD75345.1"
                     /translation="MSASADTAGFMAMKGAGYYSKATTGARDVINLAVPLIVGAIERM
                     GLADDAASFRAADLGCADGGTSVGMWRTVLGEVRRTYPTKPIEITYTDLPRNDFSQLF
                     RMIHGQTDVQSYYGEIPDVYPFASATSFHQAIFPRASMNLLFSATASHYISKVPGPIS
                     DHVHMVGASGAERAAYEKIGRVEWERFLNLRARELVRGGRLCFLNFGIDSKGRYLGHT
                     GGPSMFDTFNLLWRRLAESGRITAEEYRNTNFPQVYRTASEFTAPLRDEGSQVYRAGL
                     RLEHVEERHLECPYAVAFKAHRDAARFAREYIPTLRSWSEPTFASGLSASRPPAERAA
                     ILDEFFGSYERLVAEKPDGHGMDYVHVHLICRKD"
     gene            8969..9577
                     /locus_tag="PK264_05320"
     CDS             8969..9577
                     /locus_tag="PK264_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HRD75346.1"
                     /translation="MKAPRVPEPTELEYALASRLNVAFGRQPDVRLHGNYGGDKFLAV
                     AAFRNEPRRKLVTLTTIGLSNHDFRQGKAKAQARVTEERIEICSIVSERSKKFDLVVS
                     SCAFFIMDGLIKPIPHEVWEEKCGMINGTKWPHVFLCHAAAIDPRFATQEVSGVRINW
                     LFAAMISDSEADLVQTHGGAELESRLVSLGSGRFAINRPRVA"
     gene            9659..9916
                     /locus_tag="PK264_05325"
     CDS             9659..9916
                     /locus_tag="PK264_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75347.1"
                     /translation="MTAVNSKTVLAITNRALWRLKVVLEPEHVVYFVPRRSKLEFLCE
                     DPSMALPLEMSPDGLRRVIVWVQSDVVAVLLDGVPAVECFE"
     gene            complement(10014..10691)
                     /locus_tag="PK264_05330"
     CDS             complement(10014..10691)
                     /locus_tag="PK264_05330"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD75348.1"
                     /translation="MRGLTVVFDLDGTLVETAPDLVAATNHVLALHGTAPVELAAIRP
                     IISFGSRAMIEHGLKLNGETVSQADLDRLQARFLEFYFDNIAVGSHVFEHVGTALDEI
                     AGAGARLAVCTNKMVGPARELLGKLGLAPRFAAITGWDSFPVRKPDPAHLTGTVRLAG
                     GDLARTIMIGDNETDIATAKAAGVPVIAVTFGYTHEPVATFEPNATIDHYRELVPTIR
                     RIAAAMG"
     gene            10937..11695
                     /gene="rpiA"
                     /locus_tag="PK264_05335"
     CDS             10937..11695
                     /gene="rpiA"
                     /locus_tag="PK264_05335"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HRD75349.1"
                     /translation="MTEQPAAPDAATVESWKIAAAKRALEHVRFGMKLGLGTGSTAAH
                     FVRLLGERALKGLDVTCVPTSEATRRQAESLGLKVATLDEEPLLDLTIDGADEVDGEL
                     RLLKGGGGALLREKIVATASERMIVIADGTKAVRRLGAFPLPIEIVRFGEKATIGMID
                     MLSRDAGCSGELKVRTRADGERFITDGGNVIVDCRFGAIPDPELLDEALKLIPGVVEH
                     GLFLGLADVAIIADHSGVRMLKAAYDIPEVDAGN"
     gene            11776..11850
                     /locus_tag="PK264_05340"
     tRNA            11776..11850
                     /locus_tag="PK264_05340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11808..11810,aa:Val,seq:cac)
     gene            complement(12238..12570)
                     /locus_tag="PK264_05345"
     CDS             complement(12238..12570)
                     /locus_tag="PK264_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75350.1"
                     /translation="MRDSSPNQVSEPIRLDRLAKSIGGSLTILAALACAMAVIAEGSA
                     SRLRAAGAGKLTASAGDERHTSAAHPGRIAGFALAASSPGFAVTALRPLDLAQFRSED
                     FGDNDSGL"
     gene            complement(12684..>12871)
                     /locus_tag="PK264_05350"
     CDS             complement(12684..>12871)
                     /locus_tag="PK264_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75351.1"
                     /translation="KHDYKMDWADGVVAVEDHAVALSLAGRAHVSVVLGLRPAMKRSF
                     AWLPAPLRRAAGHLLGR"
     assembly_gap    12872..12911
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12912..13913)
                     /locus_tag="PK264_05355"
     CDS             complement(<12912..13913)
                     /locus_tag="PK264_05355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75352.1"
                     /translation="MSNLALPLVDANPMSRPASPPAALASAGASDIVVEAIRTRAGFE
                     ALEADWSDLFARAGSSTHVFQSFNWCWHWCNHFLASNDVAARGPRLAILAIRRAGRVV
                     SIWPLAEERSRGLTRLVWLGDPVSQYGDALIDTTAISPAELATALADHARRIGCHLLH
                     LRKTRADAAVAPVLAELGSTIVAREEAPYLDLASAPDFATYETRYSGHARRNRRRLLR
                     RLNEHGTAHFEYHSAGATARDLATVGISMKRAQLKSKAEVSRAVHDERFYNFFADVAD
                     GRSRPAGVVVSALRSGGEIGAVQIGVAMKGRMAMHVIVTGSKFERFGAGLLHLESTIR
                     "
     gene            complement(14020..15147)
                     /locus_tag="PK264_05360"
     CDS             complement(14020..15147)
                     /locus_tag="PK264_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HRD75353.1"
                     /translation="MKTRAAVAFEKAKPLEIVEVDLEGPKAGEVLVEIKATGVCHTDD
                     FTISGADPEGIFPAIMGHEGAGIVREVGEGVRSVKAGDHVIPLYTPECRECPSCLSRK
                     TNLCTSIRATQGQGVMPDGTSRFSYKGQKVHHYMGCSTFSNFTVLPEIAVAKVRADAP
                     FDKICYIGCGVTTGVGAVLNTAKVEPGSKAVVFGLGGIGLNVVQGLKLAGADMIIGVD
                     LNPDREAWGRRFGMTHFVNPKAIGGDVVAHLVNMTKHGADQIGGADYTFDCTGNVTVM
                     RQALEACHRGWGQSVVIGVAPAGAEIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVD
                     WYMDKKIAIDPMITHTFPLERINEAFTLMHEGKSIRSVILF"
     gene            15350..15802
                     /locus_tag="PK264_05365"
     CDS             15350..15802
                     /locus_tag="PK264_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HRD75354.1"
                     /translation="MAIAADKSRSHMDGVKMGSERGFGLVFTAVFTIIRLWPTFTFRT
                     LPALSLDSARLWALAIALACLLVALLAPAVLRPLNVLWFKFGMLLGRIMNPIVMGVLF
                     FLVFVPFAIVLRLLGKDLLRLKLDRAAPSYWIRRTPPGPTGDSLRNQY"
     gene            15840..15989
                     /locus_tag="PK264_05370"
     CDS             15840..15989
                     /locus_tag="PK264_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HRD75355.1"
                     /translation="MSLIGELWGFLRARKKFWLLPIILMMVLLGGLIVLTQGTALAPF
                     IYTIF"
     gene            16025..17866
                     /locus_tag="PK264_05375"
     CDS             16025..17866
                     /locus_tag="PK264_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HRD75356.1"
                     /translation="MRILGLSAYYHDSAAALVVDGKIVAAAQEERFTRKKHDPGFPRH
                     AAEYCLKAGGLKLSDVDYVAFYDKPFLKFERLLETYLSFAPRGFKSFQMAIPLWIKEK
                     LFQKDLLAKELKSMDPAFKGAEKLLFTEHHLSHAASAFYPSPFNEALVLTLDGVGEWC
                     TTSVGMGRGNELTIDRELHFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPKYA
                     QMIMDNLMDLKADGTFRMDLSYFDYCTGLTMTAPKFADLFGEPVRKPDKEPLTQFHMD
                     MAASVQKVTEEVMLRLTRSLQAETGAKNLCLAGGVALNCVANGKVLRDGRFEKIWIQP
                     AAGDAGGALGAALSGYYLHAGKPRVKNGNAHDQMQGSYLGPSFTDDEISKRLAKAGAR
                     FDVVAPAETIERTAKDLADGLAIGWFQGRMEFGPRALGARSILGDARSPSMQKTLNLR
                     VKYRESFRPFAPSVLREKVSEWFEHDSDSPYMLLVADVVEEKRRKMTAAEEALFGIEK
                     LNIPRSSIPAVTHVDYSARIQTVHEDTNPAYHALIERFDELTGCPVIVNTSFNVRGEP
                     IVCTPEDAFRCFMGSDIERLVVGNVILKKEDQDPRLKLDYKSAFELD"
     gene            17955..18677
                     /locus_tag="PK264_05380"
     CDS             17955..18677
                     /locus_tag="PK264_05380"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HRD75357.1"
                     /translation="MLDRLKVKIYADGADLKSMTALAANPHVKGFTTNPSLMRKAGVA
                     DYSAFARDVLKVIPDRPVSFEVFADEPAEMEAQGREIATWGRNVYVKIPVMNTKGVST
                     APIISRLSKHGVALNVTAIFTVKQVIEVTAALDFATPAVISVFAGRIADTGVDPMPMM
                     RECVQRMKAKPKAELLWASPREMLNIWHADECGCHIITVQPDVLSKLNLIGKDLDAYS
                     LETVKTFYQDATAAGYKIATRA"
     gene            complement(18709..>18992)
                     /locus_tag="PK264_05385"
     CDS             complement(18709..>18992)
                     /locus_tag="PK264_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75358.1"
                     /translation="CYAVEKTFKSGRSIMDYYAVGTHLLVGREGPRWNETSKSLIGYR
                     ILFLDHKAVGGIVLPHRDLTYFSGIEIDQRWSNFRWNEPIDDAKFQPKL"
     assembly_gap    18993..19021
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19022..21184)
                     /locus_tag="PK264_05390"
     CDS             complement(<19022..21184)
                     /locus_tag="PK264_05390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD75359.1"
                     /translation="MLARFFESPRRIATWTATTAAAVSLGLVALAPGRIAEAQQPAPA
                     VPAKSGPTLDERLSWIEKRLELLRIANGVPGLSIAVLKDDRVVLLKGFGERNIEKKLP
                     VTADTLFPIGSISKPMTALLLLMLEAEKRLSLEDRPVKHLPEMKFGDADANARVTLRD
                     LLAHRSGIERADLAWLAADGALTRKDVIAGLSNLVQVAKPGTEFHYNNYAYVAAGEAA
                     ARAAGEDFEAMIERRLFAPLGMKSTTAVYKRLHAGNDHSLGYLAATFQPPRAKDVDTT
                     VIAPAGGVISSARDMAEWVRLMIATANPAGNSPANAAMLAAWTKPQIVIGPGSSYALG
                     LFIEEWRGKKVIQHGGNVPGYSAFVAAIPELRIGFAALTNTSNSTLGGEVLMDTIFTG
                     LTEETLPPPDAALPPRRSASDAATVPMTIAGKYVYPNSDAQIVVLRGQHLMRVPGQIP
                     YLLKPLGENSFQLEGLVGYSVTIRPGTHIPSSAELFVKQPQQNLILPRRDGSTPAPTD
                     AAAKDLIARYKLANGDPVEVLPLGGKIAIVFPWQPAYPLVPDPKPDTYRMGGLKPEGA
                     WLVTPRRGRDGRIIGISIKQPNGTAEASADGLTKADITLDELRARHLAAIGGEAVLKA
                     KRTLTAEVGVTAAGMSGTLKIGYRAPLSYFGEGELKAVGKSLGMIVMRFNGVEGSVVE
                     PKTEPRKMTTNELDWHRLEATFQEPLLWTELFQSVALKG"
     gene            complement(21358..22146)
                     /locus_tag="PK264_05395"
     CDS             complement(21358..22146)
                     /locus_tag="PK264_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75360.1"
                     /translation="MTSMFSRIAGAAVVAASLLAAPMVQAQGYGPSPGYGPTPGYGSP
                     KSGYGKGDDGYGYGRHHNPDLKCWTTTSGRYYCGNQDPYDSYDRYGDRHDDRYAHRYG
                     NRYDNRYDDHYGYRYSVPPARPYGYSGYGTDEHGRTCTYDRFGRLACWYGDGSGYGAQ
                     PYQDPAEAAHYQLGRSLGMVHAVAEHCRAFDLNRPLVDAALAKAGMTLNTPTAAFQQG
                     YQLGSHLSKQIVQSYGVDGFCTVSWQRFGELGAIWPNLLVKATN"
     gene            complement(22320..>22925)
                     /locus_tag="PK264_05400"
     CDS             complement(22320..>22925)
                     /locus_tag="PK264_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75361.1"
                     /translation="HLKAAHGIDPDEIERIDIGGYRATVEVAGMPEARTPFEGKFSTA
                     FLVASALVHGSIRLDAYTPERLGDGERGTRGRAHGIERRVVGDLPQALMRRVTLAIDP
                     ECAAAFPARRSAKVAMTLRDGRRLDRYQPTRKGDPDAPLTDDELDAKFMELAGPVIST
                     SEARRLLAELWTGREGSCDLGVPDSRRHDSPKRVGGRAAAE"
BASE COUNT         3852 a         7497 c         7615 g         3857 t
ORIGIN      
        1 gaaggtgcgc gcgggccgct cgctgaagcc cgaggtctgg ccgggcggcg cgcgcgcggc
       61 cgtcgcgctt tccttcgata gcgatcatga gacctcgacg ctgcgcttcg gccagacctc
      121 gccgggcaag ctctcgatgg gcgagtacgg tgcacgcgtt ggcgtgccgc gcattcttgc
      181 gctcttgcag gcctacgata tccgcgcctc gtttttcgta ccggccgtcg tcgcgctcct
      241 gcatccggac gagcagcgcc gcgtcatcgc cgctgggcac gagatcggta tccacagctg
      301 gatccacgag ttcaactcgg agctgccggc ggcggccgag cgcgaactgc agatgcgcgc
      361 cgccgacacg ctcgagcgcg tctgcggcgt gcgtcctgtc ggcatccgca cgccatcctg
      421 ggacttctcg gcagcgacgc tcgcgatcac gcgcgagatg gggctcctct acgacagctc
      481 gctgatggcc gacgacgatc cctatgagct catcgaggac ggacgtccga caggcatcgt
      541 cgagctgccg gtcgaatgga tccgggacga tgcgccttac ctcgcgatgg accgctccgg
      601 cacggcgcgg ccctacacgc caccgctcgc cgttctcgac atcttcaagg ccgagttcga
      661 tcgcgcctac gccgacggcg gcctcttcct cctcaccatg cacccgcaca tcatcggcca
      721 ttcgagccgc atcgccatcc tcgaggcgct gatccggcac atccgccagc agcgcggcat
      781 ctggtttgcc cgacatgacg aggtcgcacg ctactgcaag gccgccgccg ggctctgagc
      841 cagcgcactg atcgaaaaga ggaatccaat catgtcgaac aagcccctcc tgcttctcca
      901 ggtcgccgat gctgcggtct acaaggccgc gctcgaccgc aagggcgtcg gccaagccgt
      961 cgacatcgag acgttccagc gcggtacgcc gccaccggat tcggcactcg cgcgcgcaga
     1021 catcctgctc gcgttcgaca ttccggccgg catgctcacc aagatgccga agctgcgctt
     1081 catccagtcg caaaccgtcg gcgtcgatgg ctggctcgcg cggggggacc tgcggcccga
     1141 tctcccgctc gcggccgcgc gtggcacgca tcgcgtgcag atgcccgaga acatcctcgg
     1201 tgcgctcttc catgcgacga agccctatgc cgcgatctcg gccgaccaga aggaaaggaa
     1261 gtggatgcgg cgcgcctccg acacgctcgc cgggaagacg cttggcatcc tggggctcgg
     1321 agcgatcggg gccgagctag cgcgcaaggc cgctgccctc gagctcacgg tgatcggcac
     1381 gaaacgcacg gtcgagccct tgccgcatgt cgccaaggtc tacgcgccgg acagcatcga
     1441 cgaggtgctc ggccgctccc acttcgtcgt gctgctgttg cccgcgaccg aggcgactgt
     1501 caacctcatg aacgcaaccc gccttgccgg catgcgcaag gacgcctggc tcctcaattt
     1561 cgcgcgcggc gcattggtcg tcgacgaaga cctcatcgcc gccgtcaagg caaggacgat
     1621 cgcgggcgcg atcctcgacg tcttccgcac ggagccgctg cctgagcagc atccattctg
     1681 gacgacgccc ggcatccacg tgctcccgca catcggcggc atgcaccccg accgcgacca
     1741 gatggtggcc gcgctcttcg ccgacaacgt cactcgcgtt ctcaatggcg agacgccgag
     1801 cgaactcgtc gatcgcaagc gcggctactg acgctgtagc ctcaaccgag cgcgtcgatc
     1861 gctgcgttca cggcgccagg cttgtgtggc accttcgcgg cgcgcatcgc aagttcgacc
     1921 gtcgccagcg cgccgagcag catcggctcg ttgaggtcgc ccatatggcc gatccggaaa
     1981 gcctttccgg cgagcggtcc gaggccgccg ccgagcgaca gattgaagcg atcctgggca
     2041 atcttgcgca tcaggttgcc gtcgatgccg tcaggcagca tgatcgccga gacagaatcc
     2101 gacgcccgcg ccggatcggc gcacagaagc tcgatcgcgc cgaccgagcc cgagagtccc
     2161 ttgaggttga gcgtcgcctt cgaccgcttg ccctcgcccc agtggctgac cgcggcccgg
     2221 acggccttgg cgaagcggct gtgacggcgg aagacggcgt cgaggccctc ctcctccatg
     2281 agcttcaaag actgctcgag cccgaagaag agatgcaccg gcgtggtgcc cgggaagcgc
     2341 tgcggggcat gcgtcaacat ctccgaccag tcccagtagc tccgtcgcat gccgcctttg
     2401 ccagcctcgg ccttccactg ggccatggcc ttctcgctga tgccggtgaa ggcgagaccg
     2461 gtcaccatca tcaggccctt ctggctgccg ccgacggcga tgtcgacgcc ccagtcgtcc
     2521 atgcggaaat cgagcgagcc gagcgaggag atcgtgtcga cgaggagtag ggccgggtgg
     2581 cgcgcggcat cgatcgcctt gcgcatgtcg ccgaccggag tggcgacacc tgtcgaggtc
     2641 tcgttgtgga cgacgagaat ggccttgatc tcggccttgc cgtccttgcg cagccgctcc
     2701 gtcagcgcct cgggcttgac gccgatgcgc cagtccgcag cgagcgtctc gacctcgagg
     2761 ccgaggctcg tcgccatgtc gccccagctc gccgagaagc ggccggattc gagcacgagc
     2821 actttctcgc cggacttcaa caggttggcg aggccggcct cccaggcgcc atggccgttg
     2881 gaggcataca tcagcatgtg ctgcttggtc ttgaggacgc gctttacact cgcgtgcacg
     2941 gaattgaaga tctcgaggaa ctcgggcgcg aagaagtcca tcgtcggccg gtgcatggcg
     3001 ccgagcacgc tatcgggaat gttcgtcggg ccgggattga agaagaacga gcggccgcgg
     3061 gggcggtcgt tgcgttgagg cgtggacatg ggcgtctccg gatggggttt gccgctccct
     3121 ggcacggcga atgagcgggc acaatgcagc ggatcgtcac cactcgtcga tcgtcggaaa
     3181 tcgcgggtct gccgtgatgc agtcggcgag atagcaggcg aggccgaggt cgccggtcca
     3241 gagcgagtag cggccgcgcc cgagtgcccg gcgtgcctcc cggcactgcg cgatggccgt
     3301 catggcgaag gcacgggccc ggtcgagcca gacggcatcg cccgtccggc gatggagctt
     3361 cagcagcgcg tagccgttgc cgacggtgcc gtgacagagg ttgctgccct tggcgagcgg
     3421 accgacgctc cagatgaagt gaccgcccgc gaccatcagc gcctctaatt cgggcgagga
     3481 gaacggcaca tcggcaaacg tggtcaccat gccgggcgcg ccgtggcagt gctggcagag
     3541 gtcgcccggc tcgccgatga cgcccggcca attggcaccg tcgtcactcg cgacggcggt
     3601 ccgttgcagc acgatcgtca cggccgcctc gacgcgcgcc tgctgcgccc gggtgagcca
     3661 atgccagccg cgcaacaggg gcaccgcatt gccggcgaag ccgtgcaccg cgccgagcca
     3721 gggcttcacg ctgccataga ggtcctgcga ccacatcggc ccgagcgcac tgtcgcgcag
     3781 gtcggcgagg acgagggcag cctgctcctc gaagagcgcg cgccaacgcg gctccccggt
     3841 cgtcgcgccc atgacgacgg ctgccagcat ggagcccggg aggccccaca tcagctcgcg
     3901 gacgggcagg gccgagttgg cgcggcagag ttcgaatatc ctgtcggctg tgccgggatc
     3961 gggcacgatc cgcatggcga ccagtgccgt gccgagcgcg ccgagcagga gcgagccatg
     4021 gcgttcgtag ccggggaaag gcgccgcgaa ctcgatgtag tcggcgtcgg aacgctcgag
     4081 aagccgcggc agcagccccg caagatcgat gccggaactg ccgtagccac gctcacagag
     4141 atgatcgagc gcccacacca caccagctgc gccgaagtag agtatggcag tgccgtcgct
     4201 gacgccgtcg tcgctcgggt gcgccggcca caagcgatcc ggcgtgaagt tcgctgccgc
     4261 atcggcaatg atcgcgcgga tctccacctc ggcctcggcg acagtccagg gggcctgatc
     4321 gagcggacgg cgccgagcgg ggtcgatcat ttgtccgggt ccggcttctg gccggtggct
     4381 ggatagaaga acgccggtcc agagccgacg atatgcgggt cgggcttgcc gacagccttc
     4441 ttgtcctttc cggcatagtc gagggcggcg agcaccacac gcatcgcctc gaggcgtgtg
     4501 cggcgcttgt cgttggcgcg gatcacggtc cagggcgagg cttgcgtatg cgtgaagcgg
     4561 aagatctcct ccttcgccgc cgtgtaggcg tcccacttgc cgatcgcggc acggtcgatg
     4621 tcggtgatct tccattgccg gagcgggtca tgctggcggc gatggaagcg cttcagctgc
     4681 atctcacggc cgatgtcgag atagaacttg aagaggtgga cgccgtcgcg gacgagcatc
     4741 tcctcgaagt gcggcgcctc gcgcaggaag tcggcgagct ggtccggcgt gcagaagccc
     4801 atcacgcgct cgacgccgcc gcgattgtac caggagcggt cgaacatcac gacgtcgccg
     4861 gccgtcggca tgtgctggat gtagcgctgg aagtaccact ggccgcgctc ggcgtcggtt
     4921 ggcttcgaga gcgccacgac gcgggcatgg cgcggattga gatgggccga gatgcgcttg
     4981 atggtcgagc ccttgcccgc ggcatcacgc ccctcgaaca ccatgaccaa gcgccgccgg
     5041 tccttttgca cggctgtctg cagcttcagg agctcgatct gcagcgtttc gaggtcgcgc
     5101 tcgtaggtgt cgcgatcgag cttcgtgtcg taggggaagc cgccgctgcc gagggccgcc
     5161 tcgtcgatcg ccttgtcgag cttcggctcc atgatgtcga atgcgtcgag gggcgacgaa
     5221 ggagccgcct tgcgtgtctt cctgggtgtg tctgccatgt tgctcgcttt actcgtgtct
     5281 cgtgatcagt cgttgtcgac gaggccggcg cccgcgcccg cgatacgcga gtggtccgag
     5341 gccggaacgt aggtgttctt ctcgagagcc tgaagcgcag cccagcgacg ctcgtcaacg
     5401 tggcaatcgg tgaccgctgc gaccgcagga ccgacgactc ggcattcaaa cgtggttgct
     5461 tcagtggcgg cgagcgcgcc ttctgccgaa acgagggcgc cgcgccagat gacgagcttg
     5521 gcgggcctgt catgcccgat ccagcttatc gccatggcct cgccgatccg gccggtgccg
     5581 acggccagca cgccggcgaa ccacacaggt gccagcatgc gcgcgcccga gaacgcgccg
     5641 cccttggcgc cgccgaaatc ggcgtaccag gccgcggccg cgatcgggtt gagctgccag
     5701 ttgtcaccgc ctatcgcgcg tacggccgcc tccgaaaagg cagcggcggc ggtctcgagc
     5761 catggcagcc cgctggcggc gagaaagcgc gcggcccgcg aggcttcctc cgcgaggccc
     5821 cagggatagc ccgcgccgcg agccgccttc aggaccgtgc cctcgatctc gttgaacgac
     5881 actctcatca ctgttcctcg ggaccgaagg gccagggcgc atcaccctgc gaggggtcgg
     5941 cggcaagttc ctccgggaga agctcggcgg caagggcacg atctgggcnn nnnnnnnnnn
     6001 nnnnnnnnnn nnngaaacgc gtcgcgccga agaaggcgag tttgcatcgc aagagatcga
     6061 tcggccgcat gcccgccgcg atcaagttgc cttcgatctc agcgtaaggg tgccgactcg
     6121 tcgtctgcac gcggcgaacc gtatggcgca actcgggata ggcgctgacg aacccggcaa
     6181 caggcatcgc cgcgtgtggc gcctcgagtg cgcggcgaag cagggtgacg tcgcgggcga
     6241 tcgcgagcgg ttgctcgcgt tcggcgccgg gctcgttcgc ccgatcgccg agccgcggct
     6301 cgagcttttc ctccgagacg taccagaaac gcgcattctc cgcgggcgag ttgaagtcga
     6361 gctcgcgnnn nnnnnnnagt gccggtcgat caaccccgcg agggtgccgc aagtcatgag
     6421 gccgtcgatc gcgtgctcgc tcgtctcgtc gacggccatg gtctcggcga gatcgtcgac
     6481 gaggtcgccg tgtggctcga gcagcagtga tacgaccatc tcctgcgctt ccatcgcaaa
     6541 gcgagcctcg gcgtacctgt agatccgatc ccagagcccg ccgacgccat cgagcgcccg
     6601 tcgcggctcg acaagcgcgt ccgcaagggt cgccagatcg cgggcgacgc cctcgatgcg
     6661 cggcgcttgc acgggatcgc ccgtctgcca gaccgggaga gctgtgcggg cgcgggccag
     6721 tgcgtcggaa aaccgcgcga gttgatcggg cgtcgcctcc gcacgtgacc tgacgcgggc
     6781 gagtgccgtc tcacgcgccc cgatccagcg tccaatcaag gtggggtgct tcaccaggaa
     6841 cggcgccatg ccgagtccgg ttgcattgcc gatgccgaag aggcggcgaa ccgcgggctc
     6901 gatcgcgacc gcgcgcgcgc caccccgcgc ctgggcgaca tgctcgacga gatcgagtgt
     6961 gaacgtgcgg atcagccaga ccgccagcat ctcggcgcga aacggtcccg caagctccgg
     7021 atggcggagc accacgtcgc gatcggcgat gccgaacttg ccgttgccgt agacggcggt
     7081 cgtgcgcatg aggtagccga cggcctcgat ggtccgccga tccggctgcc gtccggccgc
     7141 gagcgcgtcg accaccgtat cgaacaggcg cacgctgcgg ttggcgcgcg cgagcaccaa
     7201 ttccctcacg ctgtagcgtc cagcttcctg cctcggcacg ttctcgcgaa gccgcgcgat
     7261 ggccgcctcg tccggctcgc catcgtagag cgcgtagctc gtatcccagg cttccgcgat
     7321 cacgcgatcc gtacgcttct cgggggcgag cagcgtcgag aagcagacga gcgagacggc
     7381 gccactcgga agccgggccc tatagacggc ggtgccaaag ccctcgcggc agatgtcgaa
     7441 gtgcggccgg tcgaagcgcc agcgctcgcg cgcggcgcgg cgaagaagcg cgcgcagaaa
     7501 gctcaaccgc gtctgatgcg aggcccccat gcgcgcgagc cgcatcacaa cgcccggcgg
     7561 acggagcggg acggtggcgt ggtgcagctc tcgtgccgtg cccatgcttt cctaatcctt
     7621 ccggcagatc aggtggacgt gcacgtagtc catcccgtgg ccgtcgggct tctcggcgac
     7681 gagccgctcg tagctgccga agaattcatc gaggatcgcc gcgcgttcag ccggcggacg
     7741 gctcgccgag aggccgctcg caaaggtcgg ctcgctccag gagcgcagcg tcgggatgta
     7801 ctcgcgggca aagcgggcag cgtcgcgatg cgccttgaag gcgaccgcat aggggcactc
     7861 caagtggcgt tcctcgacat gctcgagccg cagccccgcg cgatagacct ggctcccctc
     7921 gtcgcggagc ggcgccgtga actccgaggc cgtccggtag acctgaggga agttcgtgtt
     7981 gcgatattcc tccgccgtga tgcgcccgct ctcggcaagc cgccgccaga gcaggttgaa
     8041 ggtgtcgaac atgctcgggc ccccggtatg gccgagatag cgccctttgg aatcgatgcc
     8101 gaaattcagg aagcagagcc gcccgccgcg gacgagttcg cgggcgcgca gattcaggaa
     8161 gcgctcccat tcgacccggc ctatcttctc gtaggcggca cgctcggcac cactcgcgcc
     8221 gaccatgtgc acgtgatccg agatcgggcc cggcaccttc gagatgtagt gcgatgccgt
     8281 cgccgagaag agcaggttca tgctcgcgcg cggaaagatc gcctgatgga acgaggtcgc
     8341 cgaggcgaac gggtacacgt ccgggatctc gccatagtag ctctgcacgt ccgtctggcc
     8401 atggatcatg cggaagagct ggctgaagtc gttgcgtggc aggtccgtgt acgtgatctc
     8461 gatgggcttc gtcgggtagg ttcgcctcac ctcgccgagc accgtccgcc acatgccgac
     8521 ggacgtgccg ccgtcggcgc agccgagatc cgccgctctg aacgaggcgg catcgtccgc
     8581 gaggcccatg cgctcgatgg cgccgacgat gagtggaact gcgagattga tgacgtcgcg
     8641 ggcgccggtc gtcgccttcg agtagtagcc ggcccccttc atggccatga acccggcagt
     8701 atcggcagag gcgctcattt tccattcccc ttggagccat cacgcgccag ggtagcattg
     8761 acgcggccgc cagcgtcaat ggaggtgatc agaggcgcgg tgatcgatcc tccgagcacc
     8821 gacgcccgct tgttggcatg agcggcacgc cgtagtgtgc aacaaattcc catgttgccc
     8881 catccgcccg agatccagct cacccattcg gccgttgctc gctgcttggc accgcgcaag
     8941 ctgcgccgtc ccaaggcaag gaaccgacat gaaagccccg cgcgttccag agccgaccga
     9001 gctggaatac gcactcgcca gccgactgaa tgttgcattc ggaagacaac cggacgtccg
     9061 tcttcatggc aactacggcg gtgacaaatt ccttgcagtc gccgcgtttc gaaatgaacc
     9121 acgcaggaag ctcgtcacgt tgacgaccat cggactttcg aaccatgatt tccggcaagg
     9181 gaaagcgaaa gctcaggcca gagtgactga ggaaagaatt gaaatctgct cgatcgtcag
     9241 cgagcggtca aagaaattcg atctcgtggt ctcgtcttgt gcgtttttca tcatggacgg
     9301 tctgatcaag ccgatccctc acgaggtctg ggaggaaaaa tgcggaatga tcaacggcac
     9361 gaagtggcca catgtctttc tatgccacgc cgcggcgatc gatccgaggt tcgccacgca
     9421 ggaggtgagc ggggtccgca taaattggtt gttcgcggca atgatatcag acagcgaggc
     9481 ggatctggtc caaactcacg gcggcgcaga actagaatct cggctggtca gcttaggctc
     9541 gggccgtttt gcgatcaacc gaccgcgtgt tgcgtgagta tcgagatgtc gcgcgcgcag
     9601 gcggcctgtc gcgtccaaca caacctcgtc cgattcgaac atcagggggg tcgtgcccat
     9661 gaccgccgtc aactcgaaaa cggtgcttgc cattaccaat cgcgcgctgt ggcgtttgaa
     9721 ggtcgtgttg gaaccggagc atgtcgtcta cttcgttccg cggcggtcga aacttgaatt
     9781 cctctgcgaa gatccgtcga tggcgctccc gctggaaatg tcccccgatg gtctacgacg
     9841 ggtcatagtt tgggtacagt cggatgtagt ggcggtactt ttagacggcg ttccagcagt
     9901 cgagtgtttc gagtaggcct gaagcgattg catcgcctcc acgatccgat gccatgtcgc
     9961 atgatggcaa gcggcgtgtc tgccctgcct tgacgtcgac cgaaccgcca cgctcacccc
    10021 atcgccgccg cgatccggcg gatcgtcggg accaactcgc gatagtgatc gatcgtcgcg
    10081 ttcggctcga aagtggcgac gggctcgtgc gtgtagccga aagtcacggc gatcaccggc
    10141 acacctgcgg ccttggcggt cgcgatgtcg gtctcgttgt caccgatcat gatggttcgg
    10201 gcgaggtcgc ccccggcgag gcggacggtg ccggtcaagt gcgcagggtc gggcttgcgg
    10261 accggaaagc tgtcccagcc cgtgatcgcc gcgaagcgcg gggcgaggcc gagcttgccg
    10321 agcagctctc gcgccgggcc gaccatcttg ttggtgcaga cggcgagccg cgcccccgcc
    10381 cccgcgatct cgtcgagcgc cgtgccgacg tgctcgaaga cgtggctgcc gacggcgata
    10441 ttgtcaaaat agaattcgag gaagcgcgcc tgcagccggt cgaggtcggc ctggctcacg
    10501 gtctctccgt tgagcttcag gccgtgctcg atcatcgcac gcgagccgaa ggagatgatc
    10561 gggcggatcg cggcgagctc gacgggggcc gtgccatgca aggcgagcac gtggttggtc
    10621 gccgccacga ggtcgggcgc cgtctccacc agcgttccgt cgagatcgaa cacgactgtc
    10681 agcccgcgca tggtctctcc tcgtcgcccg atcgccgtca tcgtcccgcg cacatttgac
    10741 gcgaaactgt ggacgacgcc acagtgtggc ctgcgacccg gttgcaacag tggcgatggc
    10801 gccgcagtgt cttcgagcgc gatcggttgt atcgatagct ggtcgacgcg gcggaatggc
    10861 gctccagcgg gggcgccagc acacatcacc catcggcgag gcgcccatgc agctgagacg
    10921 aggtaccgag agcgccatga cggagcaacc agcagcgccc gatgcggcga ccgtcgagtc
    10981 ctggaaaatt gctgcggcca aacgtgcgct cgagcatgtg cgattcggca tgaagctcgg
    11041 tctcggcacg ggctcgaccg cagcccactt cgtgcgcctc ctcggcgagc gggccttgaa
    11101 gggcctcgac gtgacgtgcg tgccgacctc cgaggcgacg cgccggcagg ccgagagcct
    11161 cggtctcaag gtcgcgacgc tcgacgagga gccgctcctc gatctcacca tcgacggcgc
    11221 cgatgaggtc gacggtgaac tgcgcctctt gaagggcggc ggcggcgcac tactgcgcga
    11281 gaagatcgtc gcgacggcct ctgagcgcat gatcgtgatt gccgacggca ccaaggcggt
    11341 gcgccggctc ggggccttcc cgctgccgat cgagatcgtc cggttcgggg agaaggcgac
    11401 gatcggcatg atcgacatgc tttcgcggga cgccggctgc tcgggcgagc tcaaggtgcg
    11461 cacgcgggcg gacggcgagc gcttcatcac cgacggcggc aacgtgatcg tcgactgcag
    11521 gttcggcgcg atcccggatc ccgagctcct cgacgaggcc ttgaagctca tcccgggcgt
    11581 ggtcgagcac ggccttttcc tcgggctcgc cgacgtcgcc atcatcgccg accactcggg
    11641 cgtgcgcatg ctgaaagccg cctacgacat tccggaggtg gatgcgggca actgaacgcc
    11701 acgtgcaaac tcacacggcg ccaactctgc ttgcgcgccg aggctgcgtt cgctaaggag
    11761 ttgcggcctt ggttcgggcg gttagctcag cggtagagca cacgcttcac acgcgtgggg
    11821 tcgcaggttc aatccctgca ccgcccacca gtttgaccga gcagtgtcag attgttgcgc
    11881 cgtggctctg gccttggtgg tcggacttct tgccgctcgc cagcatggtg ctgccgtcat
    11941 ccgcggcgag atgaccgccg atgagcgtgg tcgcgcggag aaggccattc gtgccgaccc
    12001 ggagacgcgc attcccagcg gtacccccgc agcgtcgcac gaagggctca cgccgacgaa
    12061 agcagacgct gcggcttgta tcgactgaag gttcaacccg gtggagtgcc tgcgctggca
    12121 ggatcgcaac tcgcaggcgc gcgattgtga gatcatcctg ctcgtagctc gagacacgat
    12181 cgacaagccg gatccaactg ccggatactt cggagccgaa gcctcgctcg cccggtgtca
    12241 gaggcccgaa tcgttgtcgc cgaagtcctc ggagcggaac tgggcgaggt cgagcgggcg
    12301 gagcgcggtc accgcgaagc ccggactcga ggcggcaaga gcgaacccgg cgatccgccc
    12361 tggatgggcc gccgaagtgt gacgctcgtc acccgccgaa gcggtcagct ttccggcgcc
    12421 cgcagcgcga aggcggctgg cgctgccttc cgcgatcacg gccatggcgc acgcgagggc
    12481 cgccaggatc gtgaggctgc cgccgatcga cttggcaagg cgatccaggc ggatcggctc
    12541 actgacttgg ttcggacttg aatcgcgcaa ctgaccaact ccgtcgcaga acctcagcct
    12601 cggttcgagg ctcgacttgc gacagtttgc cgtctgaaag ttagcgcccg attaagcccg
    12661 gtcctactat ggcacggccc gcctcagcgg ccgagcagat gccccgccgc gcggcggagc
    12721 ggcgccggca gccacgcgaa ggacctcttc atggccgggc gcagcccgag gacgaccgac
    12781 acgtgagccc ggccggcgag cgacagggcc acggcgtgat cctcgactgc cacgacaccg
    12841 tcggcccagt ccatcttgta atcgtgcttc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn ngcggatcgt gctctcgagg tgcaggaggc ccgcgccaaa gcgctcgaac
    12961 ttgctgcctg tgacgatcac gtgcatggcc atgcgtccct tcatggcgac gccgatctgc
    13021 acggcaccga tctcgccgcc cgagcgcaag gccgagacga cgacgccggc gggccggctc
    13081 cggccatcgg cgacatcggc gaagaagttg tagaagcgct cgtcgtggac ggctcgcgac
    13141 acctcggcct tcgacttgag ctgcgcgcgc ttcatcgaga tgccgactgt cgcgagatcg
    13201 cgcgccgtgg cgccggcgga gtggtattcg aagtgagcgg taccgtgctc gttgaggcgg
    13261 cggagcagcc gacggcggtt gcgacgggca tggccgctat aacgggtctc gtaggtggca
    13321 aagtcagggg cgctggcgag gtcgaggtag ggcgcttcct cacgggcgac gatcgtcgag
    13381 ccgagctcgg cgagcacggg ggcgaccgcg gcatcggcgc gcgtcttgcg caagtgcagg
    13441 aggtgacagc cgatgcggcg tgcgtgatca gcgagggccg ttgcgagctc ggcgggcgag
    13501 atcgcggttg tatcgatcag cgcgtcaccg tactggctca cgggatcgcc gagccagacg
    13561 agccgcgtga ggccgcgcga acgctcctcg gcgagcggcc agatgctcac gacacgtccg
    13621 gcgcgcctga tcgcgaggat cgcgaggcgc gggccccgcg cggcgacgtc gttggacgcc
    13681 aaaaaatgat tgcaccagtg ccagcaccag ttgaacgatt ggaagacgtg cgtcgatgac
    13741 ccggcgcgcg cgaagagatc gctccagtcc gcctcgagcg cttcgaatcc ggcacgcgtc
    13801 cggatcgcct cgacgacaat gtcgctcgcg ccggccgaag ccagggctgc cggcggggac
    13861 gccgggcgcg acatcgggtt tgcatcgacg agcggcagcg caaggttcga catgtccggt
    13921 cccgggatca agcgttgacc ccgtgtcaga agcaatcaac gtgccagcgg cgcccgaagg
    13981 ttgcagccgc attgcggcgc gctgcacacg ctatcccgat cagaacagga tcacgctccg
    14041 gatactcttg ccctcgtgca tcagggtgaa cgcttcgttg atgcgctcga gcggaaaggt
    14101 gtgcgtgatc atcgggtcta tggcgatctt cttgtccatg taccagtcga cgatcttggg
    14161 cacgtcagtg cgcccacgcg cgccgccgaa ggccgtaccc ttccagacgc gccccgtaac
    14221 gagttggaac ggacgcgtcg cgatttcagc acccgccggc gcgacgccga tcacaaccga
    14281 ctgcccccag ccgcgatggc aggcctcgag cgcctggcgc atcaccgtga cgttgcctgt
    14341 gcagtcgaaa gtgtagtcgg cgccgccgat ctggtcggca ccatgctttg tcatgttgac
    14401 caggtgggcg acgacgtcgc cgccgatcgc cttcggattg acgaagtggg tcatgccgaa
    14461 gcgccggccc caggcctcgc ggtcggggtt gaggtcgacg ccgatgatca tgtcggcgcc
    14521 ggcgagcttc aggccctgca cgacgttgag gccgatgccg ccgagaccga acacgacggc
    14581 cttcgatccg ggctcgactt tggcggtgtt gagcaccgcg ccgacccccg tcgtcacgcc
    14641 gcagccgatg tagcaaatct tgtcgaaggg tgcgtccgcg cgcaccttgg cgaccgcgat
    14701 ctcggggagc accgtaaagt tcgagaaggt cgagcaaccc atgtagtggt gcaccttctg
    14761 ccccttatag gaaaagcggc tcgtcccgtc cggcatcacg ccctggccct gcgtcgcccg
    14821 gatcgacgtg cacaggtttg tcttgcggga gaggcaggag gggcactcgc ggcactcggg
    14881 cgtgtagagc gggatgacgt ggtctccggc cttgacgctc ctgacgccct ctccgacttc
    14941 gcgcacgatc ccggcgccct cgtgccccat gatggccgga aatatgcctt ccggatcggc
    15001 ccccgagatg gtgaaatcgt cggtgtggca gacaccggtc gccttgatct cgacgagcac
    15061 ctcaccggcc tttggcccct cgagatcgac ctcgacgatc tccagcggct tggccttctc
    15121 gaatgcaact gcggcgcgcg tcttcatggc tgctcccgat gctcgaactc gcttgggact
    15181 atggcagggg catcgcagga tgcaaggagc cgatcgcttc ccgcctcgac acctgacgcc
    15241 aagtccccgg aagtgttaag tggcgagcca cgcgaccccc gtgtgctgtg ctggcgcttg
    15301 gcttgcatgg tggcacgaaa cggatcaacc ggccttgcgg agtgttcaca tggctatcgc
    15361 cgccgacaag tcgcggtctc acatggacgg ggtgaaaatg ggctcggaac ggggcttcgg
    15421 cctcgtcttc acggccgtgt tcacgatcat aaggctctgg ccgacgttca ccttccgcac
    15481 gctccctgcg ctgagcctcg actcggcgcg gctctgggcg ctggccattg ccctcgcctg
    15541 cctgctcgtc gcgctcctgg cgcccgcggt gctccgcccc ttgaacgtgc tctggttcaa
    15601 gttcggcatg ctgctcgggc gcatcatgaa tccgatcgtc atgggcgtcc tgttttttct
    15661 ggtctttgtg ccatttgcga tcgtcctccg cctcttgggc aaggatctac tccgcctgaa
    15721 gctcgatcgc gcggcgccct cctactggat caggcgcacg ccaccgggtc ccaccggcga
    15781 cagcctgcgc aaccagtatt gatcaccggc gccgacacca ttcggggagc aaggatatgc
    15841 tgtcgctcat cggtgagttg tggggcttcc tgcgcgcccg gaagaaattc tggctgctgc
    15901 cgatcatcct gatgatggtg ctgctcggcg ggctcatcgt gctcacgcaa ggcacggcac
    15961 ttgcaccctt catctacacc atcttctgag gcccgccttc gggccaattc ggcggagctg
    16021 tcgcgtgcgc attctcggtc tgtcggccta ctaccatgac agtgcagcgg ccctcgtcgt
    16081 cgacggcaag atcgttgccg ccgcccagga ggagcgcttc acgcggaaga agcacgaccc
    16141 gggctttccc cggcatgcgg ctgagtattg cctcaaggcc ggtgggctca agctctccga
    16201 tgtcgactac gtcgcgttct acgacaagcc gtttctgaag ttcgagcgcc tgctcgagac
    16261 ctacctttcg ttcgcgccgc gcggcttcaa gtcgttccag atggcgatcc cgctctggat
    16321 caaggagaag ctcttccaga aggacctgct cgccaaggag ctcaagtcca tggaccccgc
    16381 cttcaagggc gctgagaagc tcctcttcac cgaacaccac ctgagccatg ccgcgtcggc
    16441 cttctatccc tcgcccttca acgaggcgct ggtgctgacg ctcgacggtg tcggcgaatg
    16501 gtgcacgacc tcggtcggca tggggcgcgg caacgagctc acgatcgacc gcgagctgca
    16561 tttcccgcac tcgctcggcc ttctctactc ggccttcacc tactacacgg gcttcaaggt
    16621 gaactccggc gagtacaagg tgatgggcct cgccccctat ggcgagccga agtacgcgca
    16681 aatgatcatg gacaacctca tggacctgaa ggctgacggc accttcagga tggacctcag
    16741 ctacttcgac tactgcaccg gcctcaccat gacggcgccg aagttcgccg acctcttcgg
    16801 cgagccggtg aggaagcccg acaaggaacc gctcacacag ttccacatgg acatggcggc
    16861 ctccgtgcag aaggtgacgg aagaggtgat gctgcgcctc acgcgctcgc ttcaggcgga
    16921 aacgggcgcg aaaaacctct gcctcgcggg cggcgttgcg ctcaattgtg tcgccaacgg
    16981 caaggtactg cgcgacggcc ggttcgagaa aatctggatc cagccggcgg ccggtgatgc
    17041 cggcggcgca ctcggcgcgg cactttccgg ctactatctg cacgccggca agccgcgcgt
    17101 gaagaacggc aacgcccacg accagatgca gggctcctac ctcggaccga gcttcacgga
    17161 tgacgagata agcaagcggc tggccaaggc cggcgccagg ttcgacgtgg tggcacccgc
    17221 cgagacgatc gagcgtacgg ccaaggacct cgccgacggt ctcgccatcg gctggttcca
    17281 gggccgcatg gagttcgggc cgcgtgcgct cggcgcccgc tcgatcctcg gcgatgcccg
    17341 ctcgcccagc atgcagaaga cgctcaactt gcgcgtcaag taccgcgaga gcttccgccc
    17401 gttcgcacca tcggtgctcc gcgagaaggt ctccgaatgg ttcgagcatg acagcgacag
    17461 cccctacatg ctgctcgtcg ccgacgtggt cgaggagaag cgccgcaaga tgaccgccgc
    17521 cgaggaggcg ctgttcggca tcgagaagct caacattcca cgctcgtcca tcccggcggt
    17581 gacgcatgtc gattattcgg cgcggatcca gacggtgcac gaggacacca atccggccta
    17641 ccacgccctc atcgagcgct tcgacgagct gaccggctgt ccggtgatcg tgaacacgag
    17701 cttcaacgtg cgcggcgagc caatcgtgtg cacgcccgag gatgccttcc gctgcttcat
    17761 ggggagcgac atcgagcggc tcgtcgtcgg caacgtgatc ctcaagaagg aggatcagga
    17821 tccgcggctg aagctcgact acaagagtgc cttcgagctc gattgacggc ggcggccatt
    17881 gtaccgccga gggtattccg gttgccgctc gaagcatata aataaccggc gaccagagct
    17941 tccggaggac cacaatgctc gatcggctga aggtgaagat ctacgcggac ggcgccgatc
    18001 tgaagagcat gacggcgctc gccgcgaacc cccacgtcaa gggtttcacc acgaacccgt
    18061 cgctgatgcg caaggcgggc gtcgccgatt attcagcctt tgcccgcgat gtgctgaaag
    18121 tgatcccaga ccgacccgtc tccttcgagg tgttcgcgga cgagcccgcc gagatggagg
    18181 cgcagggccg cgagatcgcg acctggggcc gcaacgtcta cgtgaagatc ccggtgatga
    18241 acacgaaggg cgtctcgacg gcgccgatca tctcgcggct ctccaagcat ggcgttgccc
    18301 tcaatgtgac cgcgatcttc accgtgaagc aggtcatcga ggtgaccgcc gccctcgatt
    18361 tcgcgacacc cgcggtgatc tccgtgttcg ccgggcgcat cgccgatacc ggtgtcgacc
    18421 ccatgccgat gatgcgcgag tgcgtacagc gcatgaaggc gaagccgaag gccgagctgc
    18481 tctgggcgag cccgcgcgag atgctcaaca tctggcatgc cgacgagtgc ggctgccaca
    18541 tcatcacggt gcagcccgac gtgctctcga agctcaacct gatcggcaag gatctcgacg
    18601 cctactcgct cgagaccgtg aagaccttct accaggacgc gaccgcggcc ggttacaaga
    18661 tcgcgacgcg cgcttgaacg tcagcggagg cggcgatgcc gcctgcgctc acaacttcgg
    18721 ctggaacttg gcgtcgtcga tcggctcgtt ccagcggaag ttcgaccagc gctgatcgat
    18781 ctcgatgccg gaaaagtacg tgagatcgcg gtgcggcagg acgatgccgc ccacagcctt
    18841 gtggtcgagg aagaggatgc ggtagccgat gagtgacttc gaagtctcgt tccagcgggg
    18901 gccctcgcgt ccgacgagca ggtgcgtgcc gacggcgtag tagtccatga tgctccgccc
    18961 gctcttgaac gtcttttcga cggcgtagca ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19021 nccctttgag ggcaacgctc tggaacagct ccgtccagag caatggctcc tggaatgtcg
    19081 cctccagccg atgccagtcg agttcgtttg tcgtcatctt gcgaggctcg gtcttcggct
    19141 cgacgaccga accttccacg ccgttgaagc gcatgacgat catgccaagc gatttgccga
    19201 cggccttcag ttcaccctcg ccgaaatagg agagcggcgc ccggtagccg atcttgagcg
    19261 tgcccgacat gccggcagcc gtcacgccga cctcggccgt gagtgtgcgc ttcgccttga
    19321 gcaccgcctc gccgccaatc gctgcgagat gacgcgcacg aagctcatcg agcgtgatgt
    19381 cggctttggt aagtccgtcg gcgctcgcct ccgcggttcc gttcggctgc tttatcgaga
    19441 tgccgatgat gcgcccgtcc cgcccccgcc gtggagtgac gagccaggcg ccctccggct
    19501 tgaggccgcc catgcgatag gtatccggtt tcggatcggg aaccagcgga taggccggct
    19561 gccaggggaa cacgatggcg atcttgccgc caagcgggag gacctcgaca ggatcgccgt
    19621 tggcgagctt gtatctagcg atgagatcct tggcggcagc gtccgttggc gcaggggtcg
    19681 agccatcgcg tcgcggcagg atgaggttct gctgcggctg cttcacgaag agctcggccg
    19741 aggagggaat gtgcgtgccg ggcctgatcg tcacggagta gccgacgagg ccctcgagct
    19801 ggaagctgtt ctcgccgagc ggcttcagca agtaggggat ttgccccggc acacgcatca
    19861 gatgctggcc gcgtagaacg acgatctggg cgtcgctgtt cgggtagacg tacttgccgg
    19921 cgatggtcat cggcactgtc gctgcatcgg acgcgctgcg acgcggcggt agggcagcat
    19981 ccggcggcgg cagcgtctcc tcggtgaggc cagtgaaaat ggtatccatc agcacctcgc
    20041 cgccgagcgt cgagttcgac gtgttggtga gcgcggcaaa gccgatccga agctcgggga
    20101 tggcggcgac gaaggcgctg tagccgggca cgttgccgcc gtgctggatg accttcttgc
    20161 cccgccattc ctcgatgaag aggccaagcg catagctcga tccgggcccg atcacgatct
    20221 gcggcttggt ccacgccgca agcatcgcgg cattggcggg actgttgccg gccggattgg
    20281 ccgtcgcgat catcaggcga acccactccg ccatgtcccg cgcgctcgag atgacgccgc
    20341 ccgccggtgc gatgaccgtc gtgtcgacgt ccttcgcgcg cggcggctgg aaggttgcgg
    20401 ccagatagcc gagcgagtgg tcgttgccgg catgcaggcg cttgtagacg gccgtcgtcg
    20461 acttcatgcc gagcggcgca aagaggcgcc gctcgatcat cgcctcgaag tcctcgccgg
    20521 cagcgcgggc cgcagcctcg ccggcggcta cataggcgta gttgttgtag tggaactcgg
    20581 tgccgggctt ggcgacctgg accaggttcg agagacccgc gatcacgtcc ttgcgggtga
    20641 gcgcgccgtc ggccgcgagc caggcgagat ccgcccgctc gatccctgag cgatgggcga
    20701 ggagatcgcg cagtgtcacg cgggcgttgg cgtcggcatc gccgaacttc atctcgggca
    20761 ggtgcttcac cggccgatcc tcgagcgaga gccgcttttc cgcctcgagc atcagcagca
    20821 gcagcgccgt catgggcttc gagattgagc cgatcgggaa cagcgtatcc gccgtgaccg
    20881 gcagcttctt ctcgatattg cgctcgccga accccttgag cagcacgacg cgatcgtcct
    20941 tcaggacggc aatcgacagg ccggggacgc cgtttgcaat gcgcaaaagc tcgaggcgct
    21001 tctcgatcca ggaaaggcgc tcatcgaggg tcgggccgct cttggcgggg acggcaggcg
    21061 ctggctgctg ggcttcggca atgcgtcccg gcgccaatgc cacgagcccc aggctcacgg
    21121 cagccgccgt cgtcgcagtc cacgtggcaa tcctgcgggg gctctcgaag aagcgtgcga
    21181 gcatggccgt ctccgtcaat cgatcagggc gcaagagctg ccgcgggact gttcagtcgt
    21241 gcgatcgtgg aggccgccgc gcagctcgag tatgtcatgc gaacgagaaa accccggcag
    21301 caggcgctgc cggggcacat tggtttcagg agaagttccg cgacatgtgc tgcggcctca
    21361 gttggtggcc ttgacgagca ggttcggcca gatcgcgccg agctcgccga agcgctgcca
    21421 cgaaaccgtg cagaagccat caacaccgta actctggacg atctgcttag aaaggtgcga
    21481 gccgagctgg tagccctgct ggaaggcggc cgtcggggta ttcagcgtca tgccggcctt
    21541 ggcgagtgcg gcatcgacca gcggacggtt caggtcgaag gcgcggcagt gctcggcaac
    21601 cgcgtgcacc atgccgagcg agcggccgag ctgatagtgc gcggcttcgg ctggatcctg
    21661 gtagggctgc gcgccgtagc ctgagccatc gccataccag caggcgagac ggccgaagcg
    21721 gtcatacgtg caggtgcggc cgtgctcgtc ggtgccatag ccgctgtagc cgtagggcct
    21781 ggcgggcggc acggagtagc gatagccgta gtggtcgtca tagcggttgt cgtagcgatt
    21841 gccgtagcga tgggcatagc ggtcatcgtg acggtcgccg tagcggtcgt agctgtcgta
    21901 ggggtcctgg ttgccgcagt agtagcggcc cgacgtcgtc gtccagcatt tgaggtcggg
    21961 gttgtggtgg cggccgtagc catagccgtc gtcgcccttg ccgtagccgg acttcggact
    22021 gccgtagccg ggtgtcggac catagcccgg cgaggggcca tagccttggg cctgcaccat
    22081 cggcgcggcg agcaggcttg ccgccacaac ggccgctcct gcaattcgcg agaacatgga
    22141 cgtcatggca gttgctcctc tgttgcccgg tgacgtcgaa atgtgcggcg gattggccgt
    22201 cgagcgtcag ctgggggcga cccgtttcgg gtagaacctg agcgagcatt gcatcccgca
    22261 gaggcagaat tcaggaacga gaactgagtt cattgcaaat ttctccgtac ttcaagagct
    22321 tactccgccg cggcccggcc gccaactcgc ttcgggctat cgtgccgccg gctatccggg
    22381 actcccagat cgcagctccc ctcgcggccg gtccacagct ccgcgagcag ccgtcgggcc
    22441 tcgctcgtcg agatcacggg gccggcgagc tccatgaact tggcgtcaag ctcgtcgtcc
    22501 gtcagcgggg cgtcaggatc acccttcctc gtcggctggt agcgatccag cctgcggcca
    22561 tcgcggagtg tcatcgcaac cttcgccgag cgccgcgccg ggaaggccgc cgcgcactcc
    22621 gggtcgatgg cgagcgtgac gcggcgcatc aaggcctggg ggagatcgcc cacgacacga
    22681 cgctcgatgc cgtgcgcgcg gccacgagtg ccgcgttcac cgtcgccgag ccgctcgggc
    22741 gtataggcat cgagacggat gctgccatgg acgagcgcgc tcgcgacgag gaacgcggtc
    22801 gagaacttgc cctcgaaggg cgtgcgcgct tcaggcatgc cggcgacctc gacggtcgcc
    22861 cggtagccgc cgatatcgat gcgttcgatc tcgtcgggat ctatcccgtg cgccgccttg
    22921 agatg
//
LOCUS       DAORNH010000032        22598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1765_length_22598_cov_2.705940, whole genome shotgun sequence.
ACCESSION   DAORNH010000032 DAORNH010000000
VERSION     DAORNH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22598
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_22598_cov_2.705940"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1283)
                     /locus_tag="PK264_05405"
     CDS             complement(66..1283)
                     /locus_tag="PK264_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRD75362.1"
                     /translation="MKVNSRVKAIETLSCDAGWRNYHFVKLSTEDGVVGWSEFDEGFG
                     APGVGTVIQRLAPRVVGQPVGEHERIYAELYCATRPAAGGIVALAMGAIENALLDAKA
                     RSLGVPCYELLGGKIRDRIRVYWSHCATWRINQAAWYKPAITDLDGVRSIGREVREKG
                     FSAMKTNVFIYEGQKNPKGWRPGFGQPFYPELNIDRTVLKNLRMHLEAIREGAGPDVD
                     LLLDLNFHAKTEGLLKILRQIADLDMFWVEIDSYTPEALAYVRKQSPHPISSCETLLG
                     LREFIPYFREQAMDVAIIDTPWNGVWQSMKIASLAEAHEVNVAPHNFYGHLCTMMNAH
                     FAAAVPNLRIMETDIDRLEWDHELVSHVPEYKDGHLILPDRPGWGTEPVEAAIRARPP
                     KAAAGLVTPGRRT"
     gene            complement(1372..2796)
                     /locus_tag="PK264_05410"
     CDS             complement(1372..2796)
                     /locus_tag="PK264_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD75363.1"
                     /translation="MADLDYATASEIAAKIASGKVSTTEVIRSTLDRAERAQRAYNCF
                     ITIAADQALAAAAKADEAVRRGAALGPLAGVPFHVKDLVNTRGVRTTFASAIYEHNVP
                     AANAVSVDRLEAAGAIMIGKTMTPEFGHMPYTEAPLFGRTRNAYSAERTSGGSSGGAA
                     VAIAAGVAPLAIATDAGGSTRIPAACNGVVGFKQSLGLVPHDMAPDAFANLSYITPTT
                     RTVMDTALMLEVMGGAHPADPHSFAASTKGLVAAARGEGDLKGTRMAWRPFLGNTVID
                     REVLAMTEATTKVFATLGASLEAMDDDMSATEPMWLVLSTGLWNARFGDQLAKWRDRM
                     SPTLVRQMEGGGRHTSEDLARANLERTRIYRKVQGWFDRFDIIAMPTLTRTALPIEER
                     LFEPIEIEGKPVDTIRKAWYPYTHPFNLTGNPAVTLPAGFAKDGLPVAIQLVGRRGED
                     ARLLHAAALLEKAQPWSARRPEFH"
     gene            3087..3884
                     /locus_tag="PK264_05415"
     CDS             3087..3884
                     /locus_tag="PK264_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HRD75364.1"
                     /translation="MAQYENRYATATARPGTDVMVDEGLRAYMLRVYNYMAVGVAVTG
                     AVAYVMFSLMVTFDPAQAARTARGVAELKRGMFLTPLGATLLSSPIVYVMIFAPLAYS
                     IFFAWRVYNMSAAGAQVAFWVFTVIMGVCLSPIFIKFTGQSIARIFFFTAAMFAALSL
                     YGYTTKRDLSGMGTFLFMGLIGVILASVVNIWLQSPALVFALSVLGVLIFAGLTAYDT
                     QQIKDGYYMVASDGSMMQKSAIVGATHLYLDFIGMFQNLLALLGDRD"
     gene            3977..4531
                     /locus_tag="PK264_05420"
     CDS             3977..4531
                     /locus_tag="PK264_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRD75365.1"
                     /translation="MSGITVRPAIIADIPAIAAIYGHAVLTGTASFELVAPDNEEMAQ
                     RFTAVTGAGFPYLAAIDDATGALLGYAYISAYRTRPAYGNSVENSVYVSDKAKRRGVG
                     RLLLAALLERATALGFRQMIAVIGDSANMPSIGLHRALGFTFCGTIHSVGYKHGRWLD
                     SVIMQLPLGEGDRAPPRPDRMPRP"
     gene            5043..5507
                     /gene="ptsN"
                     /locus_tag="PK264_05425"
     CDS             5043..5507
                     /gene="ptsN"
                     /locus_tag="PK264_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HRD75366.1"
                     /translation="MDLGDMITLDGIIPVLRAKSKKQVLQDLAQRAATITGLPSREIF
                     ETLLQRERLGSTGVGRGIAIPHGKLPGLKRMQSLFARLETPVAFEAHDDEPVDLIFLL
                     LAPEHAGADHLKALARVSRLLREASAAEKLRSTTDRNVIYALLTQPVTSHAA"
     gene            5609..6559
                     /locus_tag="PK264_05430"
     CDS             5609..6559
                     /locus_tag="PK264_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75367.1"
                     /translation="MAVSRRKLSVVLGLAVASAGLLVLAATPRALSPERLFNAISAES
                     GIVTHRGLAYGPGPRHRLDIYRAPAGRERRALVVFYYGGGWTEGERATYAFVANALAA
                     RGYTTVVPDYRLFPEVRYPDFLDDAVAAYRWTRSSLAGGCGAPRPVVVMGHSAGAYIA
                     AMVALDGMLRRGTGAPLPAPAALIGLAGPYAFDPTTWPSTKAIFAPAAGRPDRARPVA
                     FVRGPAPPALIMHGSADTIVQLYNSRDLVAVLRASGNVAVGIEYPRLGHVGLLTAIAR
                     PLRWRGTVLDDIADFIERHAGGEAEPEACAGPPPVRRSKA"
     gene            6556..7044
                     /locus_tag="PK264_05435"
     CDS             6556..7044
                     /locus_tag="PK264_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HRD75368.1"
                     /translation="MMAPEAPIETRGAGTCCSSGVEADMATNARERRLLQVALFFAGA
                     VPVAAGLAGIILGGDLLSVTAVDAISADSHIRYLSGLLLGIGLCAWTTLPNIETAGKV
                     LRALIFAVLIGGLARLWSWIGDGAPAWPMLFGLVMELVVAPALALWQWCFVRRLSVRR
                     TT"
     gene            complement(7053..8027)
                     /locus_tag="PK264_05440"
     CDS             complement(7053..8027)
                     /locus_tag="PK264_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRD75369.1"
                     /translation="MAKISLIEAGRLAALQEMRRDSRVWVLGEDVARGGLWGQYRGFL
                     DEFGPERVVSTPISESTIMGAGLGAALVGTRPIIEMRIFDFVMCAMDELVNQIAKVRY
                     MFGGQAKPAVVVRMPHGMWRNSAAQHSQMLEAWFAHLPGVVVVAPSSPADEAGLLVSA
                     IRSDDPVLFFDPKDLWQVEGEMPAGEIKPIPFGKARMLLDGNAATVVTWSAMVPKAVA
                     AVETLAKRGHAVDLIDLRSIWPWDEDAVVKSVQKTRRLLVVHEAVEIGGFGGEIIARI
                     IDRVGPKGLDAVRRLGAPRIPIPFSPPLETAVRVTEEKIVAAMEALVR"
     gene            complement(8031..>8433)
                     /locus_tag="PK264_05445"
     CDS             complement(8031..>8433)
                     /locus_tag="PK264_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD75370.1"
                     /translation="TRARAYGMAGVKVDGNDAVAVDELATRLSGEVRSGAGPRLLHAK
                     TYRWTGHTSTDAAAWRDPKEVEAAKAGCPIARLAATLAEQGMASSELAKIKAAAEAEM
                     ADARSAARSAPFPGVGIAFEDVQDIGATRGA"
     assembly_gap    8434..8443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8444..9094)
                     /locus_tag="PK264_05450"
     CDS             complement(<8444..9094)
                     /locus_tag="PK264_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRD75371.1"
                     /translation="MAKGKTAPKAGGAVRGDNQRRLIDLYRTMVRIRAFEEAALAAHK
                     AGEMPGPLHVSLGQEGVAAGVCASLRTDDRLTSNHRGHGHALAKGADPKRMFLELLGR
                     EGGYCRGKGGSMHIADFSVGMLGANGVVGGGIPIAVGAAQGLKLMKTDAIAVCMFGDG
                     AANRGPFLEGLNWAALYKLPVLFVCEDNGVAAFTAATSVTAGPGISERAVSLGVAAT"
     gene            9597..9992
                     /locus_tag="PK264_05455"
     CDS             9597..9992
                     /locus_tag="PK264_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD75372.1"
                     /translation="MPITAFYASILAALFVILALRVIAVRRSARVALGDGGDRLLLRR
                     IRVHANFAEYAPMGLVLIGLAESVKTSSVILHFIGATLVLGRLLHAYGVSQSTEPLPV
                     RVSGMMATFAALLGAAGACLIGALRQGIF"
     gene            complement(10002..10769)
                     /locus_tag="PK264_05460"
     CDS             complement(10002..10769)
                     /locus_tag="PK264_05460"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75373.1"
                     /translation="MPRLAGKVAFITGGGGGIGRATAERFAEEGAKVVIAEINAELGE
                     AAAASARAKAGNSGGNARFVRCDITERASVDAAFAETMRTFGRLDILHNNAGGSTRQD
                     GPVTEAPDEEFWRVLKLDLYGTFLCSKVAIPLIAKSGGGSVINMSSIAALIAFPGRDC
                     YTAAKGAIASMTRSMAVEYAPKRIRVNAIAPSVTMSERLKKLMGNNPELDKLASRHVL
                     GPGYPVHIADMAVYLASDESAITTGQILSVDSGASVY"
     gene            complement(10863..11774)
                     /locus_tag="PK264_05465"
     CDS             complement(10863..11774)
                     /locus_tag="PK264_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HRD75374.1"
                     /translation="MKPLLLRLHRWIALASALPLAVVVLTALVLSFEPMLNDRDWNGR
                     SISLADVEAVIAKHDPAGKASTLNVRAYENVVMLASGKGGEMKRIDLATNQVIPADRR
                     LWSDFLTLNRRLHESLLLDLRWLVDASTIAMVVLMLIGLGMGLPFLRNTLGGWHRATA
                     WGLAPLLLLSPLTGLAIAYGITLAPPGPRIEGPPVALREAVQTVAKTHDLANVIWIRP
                     AGGATRVRVYDRGQAKVLAVTKTGLVAGPQSWPRLLHEGVWAGAWSGLLNVVVSIALV
                     LLMTTGMLIWLRRTLRRRSISARPVKA"
     gene            11966..13138
                     /locus_tag="PK264_05470"
     CDS             11966..13138
                     /locus_tag="PK264_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD75375.1"
                     /translation="MQKASASGGPLRGIRIVEMAGIGPAPLCSALLADMGADIIRIDR
                     PGATGLGFEFAGPEADIRRRGRPSVAIDLKHPDGVATVLRLIDKADALIDPFRPGVME
                     RLGLGPEICRPRNPRLVYGRMTGWGQTGPLANAAGHDINYISLSGVLHAIGTRERPVP
                     PLNVIGDMGGGAMFLLSGLLAALIEAKTSGKGQVVDVAMTEGAAYLALACFGLQARGH
                     WSESREDNFIDGGAPFWRCYETRDGRFVSIGAVEAKFYAILLDKLGLDRGMLETQFDK
                     ATWPATRELFERRFKEKTREEWCRLMEGSDICFAPVLSFAEAAEHPHAKARGSFVTVD
                     GIVQPAPAPRFSRTPSSVKGGPPAFGAQTEAVLAAWGFAAEEIRALVDNKAIGRKG"
     gene            complement(13159..13356)
                     /locus_tag="PK264_05475"
     CDS             complement(13159..13356)
                     /locus_tag="PK264_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75376.1"
                     /translation="MKAPRYGAHAVRETWVIDAKSLSTAVYRDPSPEGYRSRVDIAAG
                     DRLIPLLVPELAARLSDLDLG"
     gene            complement(13357..13662)
                     /locus_tag="PK264_05480"
     CDS             complement(13357..13662)
                     /locus_tag="PK264_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD75377.1"
                     /translation="MLEANIFTENDRIELSDGEMVRMSPQSVRHEAVKSELLDAWSDR
                     RPRHVKLAVESPLRLSALLEPAPDLFAFPRGMRLAAVGGAEVLHVVEAADTSLSYDL"
     gene            complement(13841..15289)
                     /locus_tag="PK264_05485"
     CDS             complement(13841..15289)
                     /locus_tag="PK264_05485"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HRD75378.1"
                     /translation="MATPASYLPVLNHYIDGAWVQPTSGKGQDVMNPAKNTALVKLGY
                     ASKADLDKALKAAQKGFNTWRKVSAFERSKIMRKAADILRSRIDEIAPIMTMEQGKIL
                     AEAKVELAAAADVIEWFAEEGRRAYGRIIPARADGVRNMVIQEPIGPVAGFCPWNFPV
                     TQAVRKVAGALAAGCSIIVKCPEETPGSPIGMVKCFHDAGVPAGVLNLVYGVPAEISE
                     YLIPSPIIRKISFTGSVAIGKHLNAIAAAHMKRATMELGGHAPVLVFDDVETDQVATL
                     LTGMKTRNAGQVCVSPTRFFVQEKVYEKFVAKFTDLYKSNKVGDGLDATTTMGPLANP
                     RRVNAMETLVRDAEEKGAKIATGGKRIGNQGNFFEPTVLTDVPETARVLNEEPFGPMA
                     IMLRFKDTDEVLERANKLPFGLASYAFTKNAKTATRVADALDHGMVSINHFGIALPET
                     PFGGVKDSGFGHEGGIEGLNVYMQTKFVTHMG"
     gene            complement(15366..16982)
                     /locus_tag="PK264_05490"
     CDS             complement(15366..16982)
                     /locus_tag="PK264_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD75379.1"
                     /translation="MDCDYIVVGAGSAGCVVASRLSETGASVVLLEAGPKDWHPFIHI
                     PAGVLRLLYNPAVNWMYATDPEPGTANRSIHWPRGRVLGGSSSINGMLFVRGNPADYD
                     AWAQMGCRGWSYADCLPHFKSIERYAPGDEEHRGKSGPLLLEDYRTVLPLTHRFVEAA
                     QQAGFKFTKDLSGEQPEGVGYSQMSRNGRFRGSTARTFLAAAKGRPNLRVETSAFTTR
                     LHFDGKRCTGVEFRKGGASGPLHTLRANREVIVSGGTINSPHLLQVSGIGPAAHLKSI
                     GVDVVHDLPGVGANLGDHYATRVSARVKGAVSINQLARGLGLAKELVKFVFRGNGALT
                     FGVSSAQIFCRSREGLAAPDIQLLFSPATYDEGKFGELEREPGITVAVSIARPESRGT
                     IMAKSADPFVRPSLKPNYLSASNDMRVALAGIGHVRRIFAQPALAPHFVRETLPGKDV
                     QGDDELQDFVRRTGTTIYHIVGTCKMGEDPMAVVDPRLRVRGIAGLRVVDASIMPAVT
                     TGNTNAPTIMIGEKASRMIIEDAKAVTVAA"
     gene            complement(17103..17930)
                     /locus_tag="PK264_05495"
     CDS             complement(17103..17930)
                     /locus_tag="PK264_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD75380.1"
                     /translation="MSIDQGIISHYTKGRVLERTLEALRTLGKDPTHPTIDDLAPLDE
                     FHIGGREATEHFVPLLGFRAGQRLIDVGCGVGGPARYVARSTLCHVTGIDLTPEHIEV
                     ATELSRLTGLAERTRFEVASGTALPFADGAFDGAMMLHVGMNIADKAALTREARRVLK
                     PGASFGIYDIMRTGAGDITYPCPWSGKPETSFPETPETYKRHLEAAGFTIRHEASQRE
                     FALDVGRRGREEAAKGSPMPAILRTLRGDDFSTRIGNLGAEIAAGRLAPYEIIAVAA"
     gene            complement(17965..19029)
                     /locus_tag="PK264_05500"
     CDS             complement(17965..19029)
                     /locus_tag="PK264_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HRD75381.1"
                     /translation="MAANRLIDLYSDTQTRPSAAMRKAMAEAEVGDEQRGEDPSTNQL
                     QEMTAELFGKEAAVFLPSGTMCNQIAVAVHCRPGDEIIGAETTHVFTSEGGGAAALTG
                     AQTLPIASTFGKFTGADVEAAFRDGDGRHNPTSRLVVIEQTVNRGGGNIWTEAEIADV
                     AAAAHRKGLVVHMDGARILNAVVASGVPAKRMAQHVDSVWVDLSKGLGCPVGGVLAGS
                     KAFIKDAWRWKHRFGGAMRQSGIVAAAGVYALRHNVERLAEDHANARLFAERIAAIPG
                     FKVYNPKVETNIVFFDVRGAGLSAKGVCEAILPKGVRMGTTYGSMIRAVTHHDVSKAD
                     IETAAKVLKEVAGDGQHAKR"
     gene            19503..20753
                     /locus_tag="PK264_05505"
     CDS             19503..20753
                     /locus_tag="PK264_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRD75382.1"
                     /translation="MRTPPIDLFEELDPEPRLLMGPGPVDVYPRVLRAMSTQLQGQFD
                     PQFTSYMNQTMALYRHVYRTENQWTFLIDGTARAGIEACLASIVEPGDTVLVPIFGRF
                     GHLKVEIARRVGANVVAIEAEWGTVFEPGRVEEAVRKHRPKLVAICQGDTSTTMLQPL
                     AEIGEICRRHDVLLYVDCTASLGGNPFETDAWKIDVTSAGLQKCLSGPPGIAPITFNE
                     RVVEVLKHRKHVEQGIKAPGIVEGNGPIIQSNYFDLAMLMDYWSPRRLNHHTEAATML
                     YAARECARVVLEEGLDKGIARHRLASSALRAGLEAMGLSLFGNPAFRMANVTGVIIPP
                     SIKDGEKVRADMLADFGIEIGTSFGPLAGKIWRIGTMGYVARKANVLRCLASLDAVLR
                     RNGHASKPGAVIEAAYKAYDAAAA"
     gene            20790..21374
                     /locus_tag="PK264_05510"
     CDS             20790..21374
                     /locus_tag="PK264_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75383.1"
                     /translation="MRATVAEADQMTIEEFLAFTTERPHDERWELVDGVPILNVSPTD
                     VHQIIVFDTLIALSELRRETGAAADVWCRHAGSAVATQPAETRRRREAAVGVGAPGHG
                     RGAGPIRGAVAQQHAVRSGLASQGFGEQSVPRHYVTVSQRKVDVVRHDLASGWQATRL
                     TALDAQIELAALGGSINLADLFVTLRSARLRRPE"
     gene            21371..21550
                     /locus_tag="PK264_05515"
     CDS             21371..21550
                     /locus_tag="PK264_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75384.1"
                     /translation="MTPPKPAPLLSRPPRVVNVGLAAFATDLRTAGAEVVHIHWSPPA
                     AGNARIAALLAKLGA"
     assembly_gap    21564..21573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21650..>22598
                     /locus_tag="PK264_05520"
     CDS             21650..>22598
                     /locus_tag="PK264_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRD75385.1"
                     /translation="MLVDVIPADQAMPELDERTILHSGPPIGWSRMCGPMQGAVQGIA
                     VFEGWAKSLAEAEAMAEAGAFAFHPNHHFGAVGPMTGMTTRSQPVLVVENRAFGNRAF
                     CAINEGLGKVMRFGGNDEEVLARLAWLRDEFGPLLGAAIRAKGGIPLKPIIARGLGMG
                     DEMHQRNVACSSLMMREVAPAMAEVARDAKALATCLAFIARNDQFFLNIAMAMGKAMT
                     DPARNIAGSSIVTAMCRNGTDFGIHVSGTGDKWFTAPVEMPQGLYFPGFTAADANPDM
                     GDSAIVETIGLGGWFFSGISPFSLMGAGAAKGVPGLGFKF"
BASE COUNT         3638 a         7428 c         7571 g         3941 t
ORIGIN      
        1 atatgtcgcg tccgcctcaa atccaggcat gcgtggccgt ggcgtgccgc gtcagacaag
       61 gcagctcagg tccgccggcc tggcgtgacg agcccggccg cggctttggg cggacgcgcg
      121 cggattgcgg cctcgacggg ctccgtgccc cagcccggcc ggtcgggcag gatcaggtgg
      181 ccgtccttgt actcgggcac atggctgacg agctcgtggt cccactcgag ccggtcgata
      241 tcggtttcca tgatgcgcag gttcggtacg gcggccgcaa aatgcgcgtt catcatcgtg
      301 cagaggtggc cgtagaagtt gtgcggcgcg acattcacct cgtgcgcttc ggcgaggctc
      361 gcgatcttca tcgattgcca cacaccattc cagggcgtgt cgatgatagc gacgtccatc
      421 gcctgctcac ggaaatacgg gatgaactcg cgcaatccga gcagcgtctc gcaggacgag
      481 atcggatgcg gcgactgctt gcggacgtag gccagcgcct cgggcgtgta gctgtcgatc
      541 tcgacccaga acatgtcgag atcggcgatc tggcggagga tcttcaggag gccttccgtc
      601 ttggcgtgga agttgaggtc gaggaggaga tcgacgtccg gacccgcgcc ctcgcggatc
      661 gcctccaaat gcatccggag gttcttcagc acagtgcgat cgatgttgag ctcgggatag
      721 aacggctgcc cgaagccggg acgccagcct ttcgggttct tctggccctc gtagatgaag
      781 acgttggtct tcatggcgga gaagcccttt tcgcgcacct cgcggccgat cgacctgacg
      841 ccgtcgaggt cggtgatcgc gggcttgtac caggccgcct gattgatgcg ccacgtcgcg
      901 cagtgggacc agtacacgcg gatacggtcg cggatcttgc cgccgagcag ctcgtagcag
      961 gggacgccga gcgaacgggc cttggcatcg aggagcgcgt tctcgatggc gcccatggcg
     1021 agcgccacga tgccgccggc ggcaggccgc gtcgcgcagt agagctcggc atagatgcgc
     1081 tcgtgctcgc cgaccggctg gccgacgact cgcggggcga gccgctggat cactgtgcct
     1141 acgcccggcg cgccgaagcc ctcgtcgaat tccgaccagc cgacgacgcc atcctcggtg
     1201 gagagcttga cgaagtggta gttccgccag ccggcatcgc agctgagcgt ttcgatggct
     1261 ttgacgcgcg agttcacctt cagcatcgcc ggctccgtat cgctgatcat gcaaagcata
     1321 gaccgcggct ggcggtcgcg cgacatgtct cgttgatcag cgacgcgtcg atcagtggaa
     1381 ctccggccgc cgcgccgacc agggctgcgc cttttcgagg agtgccgcgg cgtgcaagag
     1441 gcgtgcatcc tctccgcgac ggccgacgag ctgtatggcg accgggagcc catccttggc
     1501 gaagcccgcg ggcagcgtga cggccgggtt gccggtgaga ttgaagggat gcgtgtaggg
     1561 gtaccaggcc ttgcgtatgg tatcgacggg cttgccttcg atctcgatcg gctcgaacag
     1621 ccgctcctcg atcggcagcg cagtgcgcgt gagcgtcggc atggcgatga tgtcgaagcg
     1681 gtcgaaccag ccctggacct tccgatagat gcgcgtgcgc tcgaggttgg cgcgggccag
     1741 gtcctcggac gtatgccgcc cgccgccctc catctggcgg acgagcgtcg gactcatgcg
     1801 gtcccgccac ttggcgagtt ggtcgccgaa gcgcgcgttc cagagccctg tcgagaggac
     1861 gagccacatg ggctccgtcg ccgacatgtc gtcgtccatg gcctcgagcg aggcgccgag
     1921 cgtggcgaac accttggtgg tcgcctcggt catcgccagc acttcgcggt cgatcaccgt
     1981 attgccgagg aacggccgcc aggccatgcg tgttcccttg aggtcacctt cgccccgcgc
     2041 ggcggcgacg agacctttgg tcgacgcagc gaagctgtgc gggtcggccg ggtgggcgcc
     2101 gcccatcacc tcgagcatca gggccgtgtc catcaccgta cgtgtcgtcg gcgtgatgta
     2161 ggagaggttg gcgaatgcat ccggcgccat gtcgtgcggg acgaggccga gcgactgctt
     2221 gaagccgacg acgccgttgc aggccgccgg gatacgggtg gacccgcccg cgtccgtcgc
     2281 aatggcgagc ggcgccacac ccgcggcgat ggcgacggcc gccccgcccg aagagccgcc
     2341 ggaggtccgt tcggcgctgt aagcgttgcg cgtccgcccg aagagcggcg cctcggtata
     2401 gggcatatgc ccgaactcgg gcgtcatggt cttgccgatc atgatcgcgc cggcggcttc
     2461 gagccggtcg accgaaacgg cattggcggc tggcacattg tgctcgtaga tcgccgaggc
     2521 gaaggtcgtg cggacacccc tcgtgttgac caagtctttc acgtgaaacg gtacgccggc
     2581 tagcggcccg agtgccgcgc cgcgccggac ggcctcatcg gccttcgccg cggcggccag
     2641 cgcctgatcg gcggcgatcg tgatgaagca attgtaggcc cgctgcgcgc gctcggcacg
     2701 gtcaagcgtg gaacggatga cctccgttgt agaaactttg cccgaggcga tcttggccgc
     2761 gatctcgctc gcggttgcgt agtcgagatc ggccataggt cctgcccccg cgtctgatcg
     2821 aaaatatcca gccttaagcc ggctcgacga tgattttggc gcgtgtccac gagacgagca
     2881 acatctgccg cgacgagacg gctctgtgat cagtgatcac gcgacgtgat gcgtcttgat
     2941 tcgcgacgcc gcatcgccat ttcgtcaggc atcgggaaga ctacgtagtc tcccgagggg
     3001 gtgccgcatc tggtcacagg caccaaacca cagtatatag gttgcaagcc ccacgatggc
     3061 gggcttcaga ggcaaaggga acaccaatgg cccagtacga gaatcgctac gcgaccgcga
     3121 cggcgcgtcc cggcaccgat gtcatggtgg acgagggact gcgcgcctat atgctgcgcg
     3181 tctacaacta catggccgtc ggcgtcgccg tcacgggcgc cgtggcctat gtcatgttca
     3241 gcctcatggt cacctttgat ccggcgcaag cggcgcgcac cgcgcgcggc gtggctgagt
     3301 tgaagcgcgg catgttcctg acgccgctcg gcgcgacgct gctgtcgagc cccatcgtct
     3361 acgtgatgat cttcgcgccg ctcgcctact cgatcttctt cgcctggcgc gtatacaaca
     3421 tgagcgccgc cggcgcgcag gtggcgttct gggtgttcac ggtgatcatg ggcgtgtgcc
     3481 tgtcgccgat cttcatcaag tttaccggcc agtcgatcgc ccgcatcttc ttcttcacgg
     3541 cggcgatgtt cgcggcgctc tcgctctacg gctacacgac gaagcgtgat ctctcgggca
     3601 tgggcacgtt cctgttcatg ggcctcattg gcgtgatcct ggcaagcgtc gtcaacatct
     3661 ggctgcagtc gccggcactg gtgttcgcgc tctccgtact cggcgtgctg attttcgccg
     3721 gcctcaccgc ctatgacacg cagcagatca aggacggcta ctacatggtc gccagcgacg
     3781 gctccatgat gcagaagtcc gccatcgttg gcgcgacgca cctctatctc gacttcatcg
     3841 gcatgttcca gaacctgctc gcgctccttg gtgatcgcga ctgacgggtt gacagcagct
     3901 tccgagcttg cgagggcccc tattggggcc ctcgttgcgt gcaggtgaag aacctctggc
     3961 ccaaggggag gtttctgtgt caggcatcac cgtcaggccg gcaatcatcg ccgatattcc
     4021 ggccatcgct gccatttatg gccacgcagt gctcaccggc acggcgagct tcgagcttgt
     4081 cgcacccgac aacgaagaga tggcgcagcg ctttacggcg gtcaccggtg cggggttccc
     4141 ctacctggcg gcgatcgacg atgccaccgg cgcgttgctc ggctacgcat acataagcgc
     4201 gtaccggacg agaccggcct acggaaacag tgtcgagaac tcagtttacg tctccgacaa
     4261 ggccaagcgg cgcggcgtcg gacgcctgct cctcgcggcc ctgctggaaa gggcgacagc
     4321 gctcgggttc cggcagatga tcgccgtcat cggcgatagc gcgaacatgc cctcgatcgg
     4381 attgcaccgt gcgctcggct tcacgttctg cggcacgatc cattccgtcg gctacaagca
     4441 cggccgctgg ctcgacagcg tgatcatgca gctgccgcta ggcgagggtg atcgcgcgcc
     4501 gccccgacca gaccggatgc cgcgtccatg atcagcgatc acagcagcgc cggcttcgcc
     4561 gtcgacgaac cgactgcgga ccaattcagg cggagcccgg atttgacttg gattacatct
     4621 gcgactaggt tcccgccgac ttccagcact tgccgtcgtg aaagtcgatg cgttggttgt
     4681 tgggaaacct gtctcgctta agcttgccag cgcgatacgg caggtgctcg caacatggag
     4741 cgacggcgtc cgtgactact cgtcaataga tggggtgttg tgcgagtcgc gccaatggtt
     4801 cggcatagca ggtctggcgc aagtctacgc tccaagggcg gctgagagcg tcccggaaag
     4861 gctctaagtg ctgccgccct catctgaagc attcgcacta cactcgacac ggtatcgaca
     4921 tcgctcggac atcgttccga tgtcgtttcg ggaatacgaa taggaaatgt ggcattgtcc
     4981 ttgcggcaca ctgagccggc accaggggcg gtgccgagcc aacccgaggg ggttaggtcc
     5041 tcatggatct cggtgacatg ataactctcg acggcatcat cccggtgctg cgcgcgaaat
     5101 cgaagaagca ggtcctccag gatctggcac agcgcgcggc caccatcacc ggcctgccga
     5161 gtcgcgaaat tttcgagacg ctcctgcagc gcgagcgctt gggctcgacc ggtgtcgggc
     5221 gcggcatcgc cattccgcat ggcaagctgc ctggcctgaa gcggatgcaa agcctgtttg
     5281 cccggctcga gacaccggtc gcgttcgaag cgcacgacga cgagccggtc gacctgatct
     5341 tcctgctgct cgcgccggag cacgctggtg ccgaccatct gaaggcgctt gcccgcgtgt
     5401 cgaggctgct tcgcgaagcg tcggcggcag agaagctccg ctccaccacc gatcgcaacg
     5461 tgatctacgc actgctgaca cagcccgtga cctcgcacgc ggcgtagccc gacgtcggca
     5521 agcaggtgga tcagtgccgc ccgacacaac caaatcgacg cccgcctcgt agattccctc
     5581 taagacgaca gaggggttgg cgcaagccat ggcggtctcg agacgaaagc tcagcgtggt
     5641 gctcggcttg gctgtggcaa gcgccgggct ccttgtgctc gcggcgacac cccgcgccct
     5701 ctcccctgag cgcttgttca atgccatctc ggccgagagc ggcatcgtca cacaccgcgg
     5761 gctcgcatac gggcctggac cgcgccatcg cctcgacatt tatcgagccc cggccgggcg
     5821 cgagcgacgc gctctggttg tcttctacta cggcggcggt tggaccgagg gcgagcgagc
     5881 gacctacgcg tttgtcgcga acgcgctcgc ggcgcggggc tataccaccg tcgtcccgga
     5941 ctacaggctg ttccccgagg tgcgctaccc cgacttcctc gatgatgcgg tggcggccta
     6001 ccgctggacc cgatcgagcc tggctggtgg ctgtggcgca cctcggcctg tcgtcgtcat
     6061 ggggcactcg gcgggggcct acatcgccgc catggttgcg ctcgacggga tgctcaggcg
     6121 aggcaccggc gcgccgctgc cggcgcccgc ggccttgatc ggtctcgcgg ggccctacgc
     6181 gttcgacccg acgacgtggc cgagcaccaa ggcgattttc gcgccggcgg cgggccgacc
     6241 cgatcgtgcc cggcccgtcg ccttcgtgcg tggcccggcg ccgcccgctc tcatcatgca
     6301 cgggagcgca gacaccatcg tccagctcta caactcgcgc gaccttgtcg cggtgctccg
     6361 cgcatcaggc aacgtcgctg tcggcatcga gtatccgcgc ctcggccatg tgggcctctt
     6421 gaccgctatc gcccggccgc tgcgatggcg gggcacagtg ctcgacgaca ttgcggactt
     6481 cattgagcgc catgccgggg gcgaggcaga gcctgaggca tgcgccggcc ctccgcctgt
     6541 taggcggagc aaggcatgat ggcgcccgag gctcccatcg aaacgcgggg cgccggcacg
     6601 tgctgctcct ccggcgtcga ggctgacatg gcgacgaatg cgcgcgagcg acgtttgctt
     6661 caggtcgcgc tttttttcgc cggcgccgtg ccggtcgccg cgggcctcgc cgggatcatc
     6721 ctcgggggtg acctactctc cgtaacggct gtcgacgcaa tctcggccga cagccacatc
     6781 cgctacctat ccggcctgct gctcggcatc gggctctgtg catggaccac gctgccgaac
     6841 atcgagaccg ccggaaaggt gctccgcgcg ctcatttttg ccgtgctgat cggcggattg
     6901 gcgcggctct ggtcatggat cggtgacgga gcaccggcgt ggccgatgct cttcggcctt
     6961 gtcatggagt tggtggtggc gccagcgctg gcactctggc aatggtgctt cgtccgccgc
     7021 ctcagtgtgc gccggacgac ctgagtccag gctcagcgca cgagcgcctc catggctgca
     7081 acgatcttct cctccgtcac ccggacggcc gtctcgagcg gcggcgagaa ggggatcggg
     7141 atgcgcggcg cgccgaggcg tcgtacggca tcgagacctt tcggcccgac ccggtcgatg
     7201 atgcgcgcaa tgatctcgcc gccgaagccg ccgatctcca ccgcctcgtg cacgacgagg
     7261 agcctccgcg tcttctggac cgacttgacc accgcgtcct cgtcccaggg ccagatcgat
     7321 ctcaaatcga tgagatcgac ggcgtggccg cgcttggcga gcgtctccac ggcggcaacg
     7381 gccttcggga ccatcgccga ccaggtcacg acagttgcag cattgccgtc gagaagcatc
     7441 cgcgccttgc cgaaggggat cggtttgatc tcgcccgctg gcatctcgcc ttcgacctgc
     7501 cacaggtcct tcggatcgaa gaagaggaca gggtcatctg agcggatcgc cgagacgagg
     7561 aggcctgcct cgtccgcggg gctcgatggc gcgacgacca ccactccagg gaggtgcgcg
     7621 aaccacgcct cgagcatctg gctgtgctgg gcggccgaat tccgccacat gccgtgcggc
     7681 atgcgcacga cgacggcggg tttcgcctgc ccgccgaaca tgtagcgcac cttggcgatc
     7741 tggttgacga gttcgtccat cgcgcacatc acgaaatcga agatgcgcat ctcgatgatc
     7801 ggccgcgtgc cgacgagcgc tgcgccgagc cccgcgccca tgatcgtgct ctcgctgatc
     7861 ggtgtcgaga cgaccctctc gggcccgaac tcgtcgagaa agccgcggta ctggccccac
     7921 aggccgccgc gcgccacatc ctcgccgagc acccaaactc ggctgtcgcg gcgcatttcc
     7981 tggagggctg cgagacgccc cgcctcgatg agagagatct tcgccatggc tcaggctccc
     8041 ctcgtcgcgc cgatgtcctg cacgtcctcg aaggcgatgc cgaccccggg aaatggcgcc
     8101 gagcgcgcgg cggagcgggc atcggccatc tccgcttcgg ctgcagcttt gatcttcgcg
     8161 agttcactcg acgccatccc ctgttcggca agcgtggcgg cgagtcgcgc gatcgggcat
     8221 ccggccttcg ccgcctcgac ctccttcgga tcgcgccagg cggcagcgtc ggtcgaggtg
     8281 tgcccggtcc agcggtaggt cttggcgtgg aggaggcgcg gtccggcacc cgagcgcacc
     8341 tcgcccgaca gccgcgtcgc cagctcgtcg acagcgacag cgtcgttgcc gtcgaccttc
     8401 acgcccgcca tcccgtaggc cctggcgcgg gtgnnnnnnn nnncgttgca gccacgccga
     8461 gcgatacggc gcgctcggag atgccggggc cggccgtgac gctcgtcgcc gcggtgaacg
     8521 ccgcgacgcc gttgtcctcg cagacgaaca gaacgggtag tttgtagagc gccgcccagt
     8581 tgagcccctc gaggaatggt ccgcgattgg ccgcgccgtc gccgaacatg cagactgcga
     8641 tcgcgtcggt cttcatgagc ttcaagcctt gcgccgcgcc gacggcgatc gggatgccgc
     8701 cgccgaccac accgttcgcg ccgagcatgc cgaccgagaa atcggcgatg tgcatcgagc
     8761 cgcccttgcc gcggcaatag ccgccctcgc gcccgagcag ctcgaggaac atgcgcttgg
     8821 ggtccgcacc tttggcgagc gcgtggccat gcccgcggtg atttgaggtc agccggtcgt
     8881 cggtcctgag gctcgcacac acaccggcag cgaccccttc ctgcccgagc gagacatgca
     8941 ggggacccgg catctcgccc gccttgtgcg cggccagtgc cgcctcctcg aaggcacgga
     9001 tgcgcaccat tgtccgatag agatcgatga gccgccgctg attgtcgccg cgaaccgcgc
     9061 cccccgcctt gggcgccgtc ttccccttcg ccatgtcgtc tccccgagcg tgacttgctt
     9121 tggagacgat cctcgcattc gccgcgcggg gagatcaacc attgatcgca cgagctatca
     9181 cggcggctcg ggtggcacga gccgcgccag cgcatcgttc gcggcccagg tgccaggccg
     9241 catgcgccag gccttgaggt tggccgcaat cgcttcctcc gttcggccaa ctcgttcgag
     9301 gatttctgcg taggtgatca ggacgtcgac gctctatggg tagcgctcaa ctcggtcgcg
     9361 cacataggcg agagcctcgt cccactctcc cgactgaacc atcgcccacg cgaactgtcg
     9421 tcggccgcga ggcccacggc tatgctggtg acatgtatcg tcgttccggg catggggcct
     9481 tggcttgcgt aacggctgtc gatcttggct tggtctcatg tccggaaggt tccaggccca
     9541 cccggcgttc cgtagccatg tcgcgcactt caatcgtcac ccaccggatt ccatcgatgc
     9601 caatcacggc cttctacgcg tccatccttg ccgcgctgtt tgtaatatta gcgctgcgcg
     9661 tcatcgctgt caggcgcagc gctcgcgttg cactcggcga tggcggcgac cggctgctcc
     9721 tgcgccgcat acgcgtgcac gcaaacttcg ccgagtacgc gccgatggga ctcgtgctga
     9781 tcgggctcgc ggaaagcgtc aagacgagct ccgtcatcct gcacttcatt ggcgcgaccc
     9841 tcgtgctcgg tcgattgctt catgcctacg gcgtgtcgca gagcacggag ccgctgcccg
     9901 ttcgtgtgtc cggaatgatg gcaacatttg ccgctctgct cggggcggct ggcgcgtgcc
     9961 tcatcggcgc ccttcgccaa ggtatttttt gaactctgag ctcagtatac cgatgcacca
    10021 ctgtccacgg acaggatctg gccggtggtg atcgcgctct cgtcggaggc aaggtagacg
    10081 gccatgtcgg cgatgtggac gggatagccg ggcccgagca cgtggcgcga ggcgagcttg
    10141 tcgagctcgg gattgttgcc catgagcttt ttcaaccgct ccgacatcgt tacggaaggc
    10201 gcgatggcat tgacgcggat cctcttcggc gcgtactcga ccgccatcga gcgcgtcatg
    10261 gatgcgatgg cgcccttcgc tgccgtgtag cagtcgcggc ctgggaacgc gatcagcgcg
    10321 gcgatcgatg acatgttgat gacggagccg ccgccgctct tcgcgatcag cggtatggcg
    10381 acctttgagc agaggaaagt cccgtagagg tcgagcttca ggacgcgcca gaactcctcg
    10441 tcgggcgctt cggtcacggg gccgtcctgg cgggtcgagc cgccggcgtt gttgtggagg
    10501 atgtcgaggc ggccgaaggt gcgcatggtc tcggcgaaag cagcatccac gcttgcgcgc
    10561 tcggtgatgt cgcagcgcac gaagcgagca ttgccacccg aattgccagc cttcgctcgc
    10621 gccgaggcgg ctgcagcctc gccgagctcg gcgttgattt cggcgatgac gaccttggcg
    10681 ccttcctcgg caaaccgctc ggccgtggct cgaccgatgc ccccgccgcc acccgtgatg
    10741 aaggcaactt ttccggcaag gcgtggcatg ggcaggctcc tcggcagctc gtgatccttc
    10801 tgtcagagcc tatgacgaca cgccacgaga agcccatgcg tcgaatggct cgcggcgcgg
    10861 cttcatgcct tgaccggtcg cgcggaaatg gaccgacgcc ggagcgtgcg gcggagccag
    10921 atcagcatgc cagtcgtcat tagaaggacg agcgcgatcg acacgaccac gttgaggagg
    10981 ccggaccagg cgccggccca cacgccctcg tgcagcaggc gcggccacga ctgcggcccg
    11041 gcgacgaggc ccgttttggt aacggccagc accttcgcct ggccgcggtc gtagacgcgg
    11101 acacgcgtcg ccccgcccgc tggcctgatc cagatcacgt tggcgaggtc atgtgtcttg
    11161 gcaacggtct ggaccgcctc gcggagtgcc accggcgggc cctcgatgcg cgggccgggc
    11221 ggggcaagcg tgatgccgta ggcgatcgcg aggcccgtca gcggcgagag cagcaggagc
    11281 ggcgccaacc cccaggccgt ggcgcgatgc cagccgccga gcgtattgcg caagaacggc
    11341 agccccatgc cgagcccgat cagcatcagc accaccatgg cgatggtcga ggcatcgacc
    11401 agccagcgca gatcgagcag cagggattcg tggaggcgcc ggttgagcgt cagaaaatcg
    11461 gaccacagtc gccggtcggc cggaatgacc tggttggtcg cgagatcgat ccgcttcatc
    11521 tcgccgcctt tgccggacgc gagcatgacg acgttctcat aggcgcgcac gttcagcgtc
    11581 gaggcttttc cagcgggatc gtgcttggcg atcacggctt ccacatcagc gagcgagatc
    11641 gaccggccgt tccagtcgcg gtcgttgagc atcggctcga aggacagcac gagggccgtc
    11701 agcacgacca ccgcgagggg cagcgccgaa gcaagtgcga tccagcggtg caggcgcagt
    11761 agcagtggct tcatgactaa gctccctcag cggtcattgg cggaggcgcg cgcgaacgtc
    11821 ggaccctggt cgcgccatgg cggtcgcaac aggaacgagc gatcgtgcca attccgttga
    11881 ccgatgactg cacggcggtc ttcgcatgac gcatagcgat aagagccgtt aggctcgcgc
    11941 gcagaaccat gcggagcagc agaccatgca aaaggcgagc gcgagcggcg gaccgctcag
    12001 aggcatcagg atcgtcgaga tggcggggat aggccccgcg ccgctctgct ccgcgctgct
    12061 cgccgacatg ggtgccgaca tcatccgcat cgaccggccg ggcgcgacgg gcctcggctt
    12121 cgagttcgcg gggccggagg ccgacatccg ccggcgcggg cgtccctccg tcgcgatcga
    12181 tctcaaacat ccggacggcg ttgccacggt gctgcggctg atcgacaagg ccgatgcgct
    12241 gatcgatccg ttccggccgg gcgtcatgga gcggctcggc ctcggcccgg agatctgtcg
    12301 gccacgcaat ccgcgcctcg tctacggccg catgaccggc tgggggcaga cggggccgct
    12361 cgcgaacgcg gccggccacg acatcaacta catctcgctc agcggcgtct tgcacgccat
    12421 cggcacgcgc gagcgccccg tgccaccgct caacgtgatc ggcgacatgg gtggcggcgc
    12481 gatgttcctg ctctcgggac tgctcgccgc actcatcgag gcgaagacct cagggaaggg
    12541 gcaggtggtc gacgtcgcca tgacggaagg cgccgcctac ctggcgctcg cctgcttcgg
    12601 ccttcaggcg cgcggccact ggtcagagag ccgcgaggac aatttcatcg acggcggcgc
    12661 gccgttctgg cgctgctacg agacgcggga cgggagattc gtctcgatcg gcgccgtgga
    12721 agccaagttc tacgcgatcc tcctcgacaa gctcggcctc gatcgcggca tgctcgagac
    12781 gcagttcgac aaggccacat ggccggcgac gcgcgaactc ttcgagcgga ggttcaagga
    12841 gaagacgcgc gaggagtggt gtcgcctcat ggagggaagc gacatctgtt tcgcgccggt
    12901 gctgagtttc gccgaggcgg ccgagcatcc gcacgccaag gcgcgcggca gcttcgtcac
    12961 cgtcgatggc atcgtgcagc ccgcgcccgc gccgcgcttc agccgtacgc cgtcgagcgt
    13021 caagggcggg ccgccagcat tcggcgcgca gaccgaggcg gtgctggcgg catgggggtt
    13081 tgcagcggag gagatccggg cgctggtgga caacaaggca atcgggcgga agggctgacc
    13141 cgaaatgccg cgcggatctc agccgagatc gagatcgctg aggcgagcgg cgagctccgg
    13201 gacgagcagc gggatgaggc gatcaccggc cgcgatatcg acgcggctcc gatatccctc
    13261 gggcgaggga tcgcggtaca ctgccgtcga gagcgacttt gcatcgatca cccaagtctc
    13321 acgcacggcg tgtgcgccgt atcgcggtgc cttcacctag agatcgtagg agaggctcgt
    13381 gtccgccgcc tccacgacgt gcaacacttc ggccccgccg actgcggcga ggcgcatgcc
    13441 gcgcggaaat gcgaagaggt ccggagcggg ctcgagcaat gccgacagcc ggagcgggga
    13501 ctcgacggcg agcttcacat gccgcggacg ccggtcgctc cacgcgtcca gcaactcgct
    13561 cttcaccgcc tcatggcgca cgctctgcgg cgacatccgc accatctccc cgtcgctcag
    13621 ctcgatccgg tcgttctcgg tgaagatgtt cgcttcgagc atcccctgaa gatcgctggc
    13681 agtgagcctc cgacgctcgg cgtcggccgg gaggggagca aggcgcggca ggttgctcga
    13741 tcgcatgttc gtggcacgag gctacaccat cgctcgcgcc aagcgcaaag ggcgagccaa
    13801 cggcccgccc ccccgcgcga tccagatcgc ggcgtccccc tcaacccatg tgcgtcacga
    13861 acttcgtctg catgtagacg ttgaggcctt cgatgccgcc ctcgtggccg aagcctgaat
    13921 ccttgacacc gccgaagggt gtctcgggga gcgcgatgcc gaagtggttg atcgacacca
    13981 tgccgtgatc gagcgcgtcg gcgaccctgg tcgcggtctt ggcgttcttg gtgaaggcgt
    14041 agctcgcgag gccgaacggc agcttgttcg cccgctccag cacctcgtcg gtgtccttga
    14101 agcgcagcat aatcgccatc ggcccgaacg gctcctcgtt gaggacgcgc gccgtctcgg
    14161 ggacgtccgt caggacggtc ggctcgaaga agttcccctg gttgccgatg cgcttgccgc
    14221 cggtcgcgat cttggcgccc ttctcctcgg cgtcacgaac cagcgtctcc atggcgttga
    14281 cgcggcgcgg attggcgagc gggcccatgg tcgtcgtggc gtccagacca tcgccgacct
    14341 tgttcgactt atagaggtcg gtgaacttcg cgacgaactt ttcgtagacc ttctcctgca
    14401 cgaagaagcg ggtcggcgag acgcagacct ggccggcgtt gcgcgtcttc atgccggtga
    14461 gaagtgtcgc cacctgatcg gtctcgacgt cgtcgaagac caggaccggc gcatgcccgc
    14521 cgagctccat cgtcgcgcgc ttcatgtgcg ccgcggcgat tgcattgaga tgcttgccga
    14581 tcgcaaccga gccggtgaag gagatcttgc ggatgatcgg cgaggggatc aggtactccg
    14641 agatctcggc tggcacgccg tagacgaggt tgagcactcc cgccggcacg cccgcgtcgt
    14701 ggaagcactt caccatgccg atcggcgagc cgggcgtctc ctcagggcac ttcacgatga
    14761 tggagcagcc ggccgcgagc gcgcccgcga ccttgcgcac tgcctgcgtc acggggaagt
    14821 tccacgggca gaagccggcg accggtccga tcggctcctg gatcaccatg ttgcgcacgc
    14881 catcggcgcg ggccgggatg atgcggccgt atgcgcggcg gccctcctcg gcgaaccact
    14941 cgatgacatc ggcggcggca gcgagctcga ccttggcctc ggccaggatc ttgccctgct
    15001 ccatggtcat gatcggcgcg atctcgtcga tgcgcgagcg caggatatcg gccgccttgc
    15061 gcatgatctt cgagcgctcg aaggcggaga ccttgcgcca ggtgttgaag cccttctggg
    15121 cggccttcag cgccttgtcg aggtcggcct tggaggcata gccgagcttc acgagggccg
    15181 tgttctttgc cgggttcatg acgtcctggc ccttgccgga ggtcggctgc acccaggcgc
    15241 cgtcgatgta gtgattgagg acggggagat agctggcagg cgtcgccatt ggttaatcct
    15301 tccctggtgg atcggcattt gtggtgaggt gtagggggag cgcgcgggaa gcgcaagaat
    15361 atgcctcaag ccgccactgt caccgccttc gcgtcttcga tgatcatgcg cgaggccttt
    15421 tcaccgatca tgatcgtcgg cgcgttggtg ttgccggtgg tgactgccgg catgatcgag
    15481 gcgtcgacga cgcgcaggcc ggcgatgccg cgcacgcgga gccgcggatc gacaacggcc
    15541 atcggatcct cgcccatctt gcaggtgccg acgatgtgat agatcgtcgt gcccgtccgc
    15601 cgcacgaaat cctgcagctc gtcgtcgccc tgcacgtcct tccccggcag cgtctcgcgc
    15661 acgaaatgcg gggcgagtgc cggctgcgcg aagatgcggc gcacatggcc gatgccggcg
    15721 agcgcgacgc gcatgtcgtt cgatgctgat agatagttgg gcttcagcga cggtctgacg
    15781 aacggatcgg ccgacttcgc catgatcgtg ccgcggctct cgggccgtgc gatcgagacg
    15841 gcgaccgtaa taccgggctc gcgctccagc tcgccgaact tgccttcatc gtaggtcgcc
    15901 ggcgagaaca aaagttggat gtcgggggcg gcgaggccct cgcggctccg acagaagatc
    15961 tgcgcgctcg agacgccgaa ggtcagtgcg ccgttgccgc ggaagacgaa tttgacgagc
    16021 tccttggcga ggccgagccc acgcgcgagc tggttgatcg agacggcgcc cttgacgcgc
    16081 gcggagacgc gggtcgcgta gtgatcgccg aggttcgcgc cgacgccagg cagatcgtgg
    16141 acgacgtcga caccgatcga tttcaggtgg gccgccggtc cgatccccga gacctgaaga
    16201 aggtgcggcg agttgatcgt gccgcccgag acgatcacct ctcggttcgc gcggagcgtg
    16261 tgcagcggac cgctggctcc acccttgcgg aactcgacgc cggtgcagcg cttgccgtcg
    16321 aagtggagcc gcgtcgtgaa ggcactcgtc tcgacgcgca agttcggccg gcccttggcg
    16381 gcggcgagga acgtgcgcgc cgtggagccg cggaagcggc cattgcgcga catctgcgag
    16441 tagccgacgc cttcgggctg ctcgcccgaa agatccttcg tgaatttgaa gcccgcctgc
    16501 tgcgccgctt cgacgaagcg atgcgtgagc ggcagaacgg tgcgataatc ctcgagcagc
    16561 aatggtcccg actttccgcg atgttcttcg tcgcccggcg catagcgctc gatcgatttg
    16621 aagtgcggga ggcaatcggc gtagctccag ccgcggcagc ccatctgcgc ccatgcgtca
    16681 tagtcggccg ggttgccgcg gacgaacagc atgccgttga tcgaggacga gccgccgagc
    16741 acgcgtccgc gcggccaatg gatcgagcgg ttcgcggtgc ccggctccgg gtcggtcgca
    16801 tacatccagt tgacggccgg attgtagagg agcctcaaca cgcccgcggg aatgtggatg
    16861 aaggggtgcc agtcctttgg gcccgcttcg agcagcacga cgctcgcgcc tgtctcggaa
    16921 agccggctcg cgaccacgca tccggccgag ccagcgccga cgacaatgta atcgcaatcc
    16981 atgtcccgcg ccttccagct cgccaatctg actgcctcgg gcgccaccat agatcagtcg
    17041 cggctccgcg gacgaggggg tgtcacgcag aggcgggtgc gggcgcgcgc atggcgcgcg
    17101 actcacgcgg cgactgcgat gatctcgtag ggcgcgagcc ggccggcggc gatctcggcc
    17161 ccgagattgc cgatccgggt cgaaaaatca tcgccccgga gtgtgcgcag gatcgccggc
    17221 atcgggcttc ctttggccgc ctcctcgcga ccgcgcctgc cgacgtcgag cgcgaactcg
    17281 cgctggctcg cctcatggcg gatcgtgaac cccgcggcct cgagatgacg cttgtaggtc
    17341 tcgggcgtct cggggaagct cgtctcgggc ttccccgacc aagggcaggg ataggtgatg
    17401 tcccccgcgc cggtccgcat gatgtcgtag atgccgaacg atgcgcccgg cttcaggacc
    17461 cggcgcgctt cgcgtgtgag cgccgccttg tcggcgatat tcatgccgac gtgcagcatc
    17521 atcgcgccgt cgaaggcgcc atcggcaaat gggagcgccg tcccgctagc gacttcgaag
    17581 cgcgtgcgct cggcaagccc cgtgagacgc gagagctcgg tcgcgacctc gatgtgctcg
    17641 ggggtgaggt cgatgccggt gacatggcag agcgtcgatc tcgccacgta gcgcgcgggg
    17701 ccgccgaccc cgcagccgac gtcgatgagg cgctggcccg cgcggaagcc gaggagcggc
    17761 acgaaatgct cggtcgcctc gcggccgccg atgtggaact cgtcgagtgg cgccagatcg
    17821 tcgatggtcg ggtgcgtggg gtccttgccg agcgtgcgga gcgcctcaag cgtccgctcg
    17881 aggacgcggc ccttggtgta gtggctgatg atgccttgat cgatcgacat gcaagcctcc
    17941 tgattgatgc gcaggcccgc ccgttcagcg cttggcgtgc tgtccgtcgc cggcgacctc
    18001 cttcaggacc ttcgcggcgg tctcgatgtc ggccttcgag acgtcgtggt gggtgacggc
    18061 ccggatcatg gagccgtaag tcgtgcccat gcgcacgcct ttgggcagta tcgcctcgca
    18121 gacgcccttg gccgaaaggc cggcgccgcg cacgtcgaag aacacgatat tggtctcgac
    18181 cttgggattg tagaccttga aaccagggat ggccgcgatg cgctcggcga aaaggcgcgc
    18241 attggcgtga tcctcggcga ggcgctcgac gttgtggcgc aacgcgtaga cgccggccgc
    18301 cgcaacgatg ccggattggc gcatggcgcc gccgaagcga tgcttccacc gccaggcgtc
    18361 cttgatgaag gctttcgagc cggcgagcac gccgccgacc gggcagccga gacccttgga
    18421 gaggtcgacc cagaccgaat cgacatgctg cgccatgcgc ttggccggaa cgcctgaggc
    18481 gacgacggcg ttcaggatgc gcgcgccgtc catgtgcacg acgaggccct tccggtgggc
    18541 ggccgcggcg acatcggcga tctcggcctc ggtccagatg ttgccgccgc cacgattgac
    18601 cgtctgctcg atgacgacga gcctgctcgt cggattgtgc cggccatcgc catcacgaaa
    18661 tgcggcctcg acatcggcgc cggtgaactt gccgaacgtg ctggcgatcg gcagcgtctg
    18721 ggcgccggtg agcgctgcgg cgccaccgcc ttcggacgtg aacacgtgcg tcgtctcggc
    18781 gccgatgatc tcgtcgcccg gacggcaatg aacggcgaca gcgatctggt tgcacatcgt
    18841 gccggagggc aggaagacgg cggcttcctt gccgaagagc tcggccgtca tctcctgcag
    18901 ctggttggtc gaggggtcct cgccgcgctg ctcgtcgccg acctcggcct cggccatggc
    18961 cttgcgcatg gcagccgacg ggcgtgtctg ggtatcgctg tagagatcga tcagtcggtt
    19021 ggcagccatg ggaccctctg tgctcgcgtg ctgacccgtg cttcgtggcc aatgttgcgg
    19081 aggcggcgac agagagcaag gcgaatgcgg attcccaaga tcgcaaaaaa aagaaagccg
    19141 ggcgcagggc ccggccgaaa gttagctcac gcgccacttg ggcgcgctga aggcgtcaaa
    19201 gggagggtac tgccttcatc agctgtccgt cacagctgtt aagactatcg caactgcgaa
    19261 atgggcgagc aatcggttgg gccgcatgcg agctatgcga tttgggtatg tcaagttgtt
    19321 tagccgccaa tcagcatgat gtagacgaaa gtcgaatatg acatgccccg gcggctaggc
    19381 ccgcccgtgc agaggctcgg caacgtcgtg gccttcgctc ttggcttgcc gtcgacgcgg
    19441 gggcggggca agtagtcgtg acgttgaccg ccgccacact cacaagcccg gtatgcagag
    19501 ccatgagaac tccccccatc gatctgttcg aggagctaga cccggagccg cgcctgttga
    19561 tgggccccgg tccggtcgat gtctacccgc gcgtgctgcg cgccatgtcg acgcaactgc
    19621 agggccaatt cgatccccag ttcaccagtt acatgaacca gacgatggcc ctttatcgcc
    19681 atgtctatcg taccgagaac cagtggacgt ttctgatcga tggtaccgcg agggccggca
    19741 tcgaggcctg cctcgcatcc atcgtcgaac caggtgacac ggtgctcgtg ccaatcttcg
    19801 gccgcttcgg gcacttgaag gtcgagattg cccgccgcgt cggagcaaac gtggtcgcca
    19861 tcgaggcgga gtggggcacg gtcttcgagc ctggccgggt tgaggaggcc gttcgaaagc
    19921 atcgaccgaa gctcgtcgcc atctgccagg gcgacacctc gaccaccatg ctgcagccgc
    19981 tcgccgagat cggcgagatc tgtcgccgcc acgacgtgct gctctatgtc gattgcacgg
    20041 cgtcgctcgg tggcaatccc ttcgagacgg atgcctggaa gatcgacgtc acatcggcag
    20101 ggctgcagaa gtgcctgtcc gggccgccgg gcatcgcgcc gatcactttc aacgagcgtg
    20161 tcgtcgaggt cctgaagcac aggaagcacg tcgagcaagg catcaaggcc cctggaatcg
    20221 tcgaaggcaa cgggccgatc atccagtcga actatttcga cctcgcgatg ctcatggact
    20281 actggtcgcc gcgccggctt aaccatcaca ccgaggcggc cacgatgctc tatgccgcgc
    20341 gcgagtgcgc ccgcgtcgtc ctcgaggaag gcctcgacaa gggcatcgca cgccatcgcc
    20401 tcgcgtcgtc ggcgttgcgc gccggcctcg aggcgatggg gttgagcctc ttcggcaatc
    20461 cggcgttccg gatggcaaat gtaaccggcg tgatcattcc gccatccatc aaggatggtg
    20521 agaaagtgcg ggccgacatg cttgccgatt tcggtatcga gatcggcacg tcgttcgggc
    20581 cgctcgccgg gaagatctgg cggatcggca cgatggggta cgtggcccgc aaggccaacg
    20641 tgctgcgctg cctcgcctcg cttgatgcgg tgttgcgccg gaatgggcat gcctcgaagc
    20701 cgggcgccgt tatcgaagcg gcctacaagg cctatgatgc cgcggcggcc tgatggccgc
    20761 atttcgagta gcgggaaatg gatcagccga tgcgcgcgac cgttgccgaa gccgaccaga
    20821 tgacgatcga ggagttcctc gccttcacga cggagcgccc ccatgatgag cgctgggagc
    20881 tggtcgacgg agtgccgatc ttgaacgtct cgccgaccga cgtccatcag atcattgtgt
    20941 tcgacactct gatcgcgttg agcgaattgc gacgcgagac cggcgcggcg gccgatgtct
    21001 ggtgtcggca cgcgggttcc gctgtcgcca cgcagcctgc cgaaacccga cgtcgtcgtg
    21061 aagcagcagt cggcgtcggg gcgcccggtc acggacgagg cgctggtcct attcgaggtg
    21121 ctgtcgccca gcaacatgct gtccgatcag gcttggcgtc gcaaggtttc ggggagcaat
    21181 ccgtacctcg ccactacgtg accgtgtcgc agcgcaaggt cgacgtcgtg cgtcacgatc
    21241 tcgcctcggg ctggcaggcg acgcggctga cagctctcga tgcacagatc gaactcgcag
    21301 cgctcggcgg ctcgatcaac ctcgccgacc tcttcgtgac actccgctcg gctcgcctga
    21361 ggaggcctga atgacaccgc caaagcccgc gcccttgttg tcgcgtccgc cgcgcgtcgt
    21421 gaacgtcggt ctcgccgcat tcgccaccga tctgcggacg gcgggcgccg aggtcgtgca
    21481 catccattgg tccccgcccg cggcaggcaa tgcgcggatt gcggcgctgc tcgccaagct
    21541 cggcgcctga ggcccggagg aaannnnnnn nnnccccgcc gcgcggcccg acgcgccggt
    21601 gttgatcttc ttccacggcg gctattggcg catcctcgcc gccgagccgg tgctggtcga
    21661 cgtcattccg gccgaccagg ccatgccgga gcttgacgag cgcaccatcc tccattccgg
    21721 cccgccgatc ggctggtcgc gcatgtgcgg gccgatgcag ggtgccgtgc agggtatcgc
    21781 cgtgttcgag ggctgggcaa agtcgctcgc cgaggccgag gcgatggccg aggccggcgc
    21841 cttcgcattc cacccgaacc atcacttcgg cgccgtcggg ccgatgaccg gcatgacgac
    21901 gcgctctcag cctgtgctgg tggtcgagaa ccgcgctttt ggcaatcgtg ccttctgcgc
    21961 gatcaatgag ggtctcggca aggtgatgcg ctttggcggc aacgacgagg aggtgctcgc
    22021 ccggctcgcc tggttgcgtg acgagttcgg cccgctcctc ggagcggcga tccgcgcgaa
    22081 gggcggcatt ccattgaagc cgatcatcgc gcgcggcctc ggcatggggg acgagatgca
    22141 ccagcgcaac gttgcctgct cgagcctgat gatgcgcgag gtcgcccccg ccatggccga
    22201 ggtcgctcgc gacgccaagg cgctcgccac gtgcctcgcc ttcatcgcgc gcaatgacca
    22261 gttcttcctg aacatcgcca tggccatggg gaaggccatg acggatcccg cgcgcaacat
    22321 cgcgggctcc tcgatcgtca ccgccatgtg ccgcaacggc acggatttcg gcatccacgt
    22381 ctcgggcacg ggtgacaaat ggttcacggc gcccgtcgag atgccgcaag ggctttattt
    22441 cccggggttc acggcggcag acgccaatcc cgacatgggt gattccgcaa tcgtcgagac
    22501 catcggtctc ggcggctggt tcttctccgg catcagcccg ttctcgctga tgggcgccgg
    22561 cgccgcgaag ggcgtgcccg gcctcggttt caaattcg
//
LOCUS       DAORNH010000033        22394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1789_length_22394_cov_2.600698, whole genome shotgun sequence.
ACCESSION   DAORNH010000033 DAORNH010000000
VERSION     DAORNH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22394
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_22394_cov_2.600698"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PK264_05525"
     CDS             complement(<1..296)
                     /locus_tag="PK264_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75386.1"
                     /translation="MTFSRDRLRAVVRLIAAGLLLALTAAAASAQRNAPAWLPHGTLL
                     EMHEALIWAANLNAEAGQVPRGELDDAIKRFRARIGSPQSGPITISEHEELLAQS"
     gene            767..1183
                     /locus_tag="PK264_05530"
     CDS             767..1183
                     /locus_tag="PK264_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75387.1"
                     /translation="MAELLIALDLADPHQGKACIVDSLMRLGEAWARPLETVWVVRTE
                     RSAAAVEAELSQCLGPDDGLLVQPIAEQGALVNASLRWFRRRVDVDAAERAERATGHA
                     PGREGGVRAPSNVVTLRVAPSPWLQVARENAVRAAS"
     gene            complement(1167..1820)
                     /gene="hisH"
                     /locus_tag="PK264_05535"
     CDS             complement(1167..1820)
                     /gene="hisH"
                     /locus_tag="PK264_05535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRD75388.1"
                     /translation="MQRIAIVDYGSGNLHSAAKAFERAARDCARPTRIEVTSDPVAVA
                     GADRIVLPGVGAFADCRRGLDAVPGMVEALQRNVVEAGRPFLGICVGMQLLAARGLEH
                     GVHPGLSWIGGDVDLIRPADPGLKIPHMGWNTIRPERPHALLANLATGEQGLHAYFVH
                     SYHLTNAPAGTLVAETEYGGPVTAIVGRDNIAGTQFHPEKSQRLGLGLITNFLSWRP"
     gene            complement(1827..2327)
                     /locus_tag="PK264_05540"
     CDS             complement(1827..2327)
                     /locus_tag="PK264_05540"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HRD75389.1"
                     /translation="MQTYTVHERPNPPGDRLERAEKLEFVKEGFDWLAAALTPLWLVV
                     QRLWLVLIAYIVIVGGLRLGLSATDADPRIWQILTVAMHLIIGLEADTLRRWTLDRNG
                     YTQIGSVVGRTVDECERRFLDVWLPEQPSGPLPTRPLGAAAASFATTVGAERAGRATT
                     LAGWRR"
     gene            2530..3225
                     /locus_tag="PK264_05545"
     CDS             2530..3225
                     /locus_tag="PK264_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD75390.1"
                     /translation="MLPIAVLFVLALAGPAASQSTGEPPRSEQPKKSEPPPGKGAPAT
                     PPTGKGATAPNAADPLKLNQIPETSEAKVKLLRELYALLATAEDAESANRISSRIERL
                     WSHSGSDTVTLLMERANAAMSNKKAELALELLDRAVKLAPDYPEAFNRRAFVHFTENN
                     FAGAVGDLRRALALDPNHYKAMDGLVQIWREVGNKRGAYYVLKQLVDVHPFWAGAKQV
                     LEELKKEVEGQDI"
     gene            3274..3666
                     /locus_tag="PK264_05550"
     CDS             3274..3666
                     /locus_tag="PK264_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75391.1"
                     /translation="MRNPIEFGRRSTPRARTLAMAGAAVGVAGGAFMAFGDSLERYSG
                     VDLAVPSCAEPAAVSDLRRIMDDYYAKKPERVKVRAIEAPKLTRHSSTEAHCEGRVRL
                     TDGKEYALRYRLYKAERQLRIQFSVSDS"
     gene            3789..4439
                     /locus_tag="PK264_05555"
     CDS             3789..4439
                     /locus_tag="PK264_05555"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD75392.1"
                     /translation="MSASTLSRRAMLATTGAGVMAAMSGVPAQALDVPKPGAAAPEFT
                     LNDSTGKPVTLASFKGTPVILEWTNHECPYVRKHYGAGNMQELQREAAAKGFAWLTVI
                     SSAPGTQGHVDGLEADQLTATRKAAPKAVLFDPTGVVGRRYGATATPNMYVIDKAGML
                     IYMGAIDDKPSTNPADIKGANNYVRAAMTAVLAGSTPAVRSTRAYGCSIKYAPVRS"
     gene            complement(4820..5821)
                     /locus_tag="PK264_05560"
     CDS             complement(4820..5821)
                     /locus_tag="PK264_05560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HRD75393.1"
                     /translation="MRALTFDRYAKRDNIAFADIPRPTPKPDEILVQVHAVGLNPVDL
                     MIPKGTFKPFLKFDLPATLGSDLAGVVIDVGSHVTRFKPGDAVYASIFDTGLGSLAEF
                     AVVPESAAAPKPATLDFVQAASIPMVGLTSWQALKVRANLKSGQRVFIPAGSGGIGTF
                     AIQLAKHLGGKVGTTTSTANVDLVRSLGADEVVDYKKQQFENVLRDYDAVLGTVRGDA
                     LKKSVRILKPKSTIVSLVGPPDAAFAGNRGMNFVLKALFGVLSRKINRLARKQGATYS
                     FLFVHPDGGQLAEIGALLDAGRIKPVIDRIFPFEQAKEALAYLEQGRAKGKVVVRLL"
     gene            complement(5872..6690)
                     /locus_tag="PK264_05565"
     CDS             complement(5872..6690)
                     /locus_tag="PK264_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRD75394.1"
                     /translation="MTKANSKIAIVTGASSGIGEATAARLATAGYKVYGTSRRGAKSG
                     RPPFEMLPLDVTSDESVMALVGDVILRDGRIDLLVNNAGFGIAPAGAEESSLEQARAI
                     FETNFFGLVRMTRAVVPHMRKQASGRIINIGSVLGFLPMPYGALYSATKHAVEGYSES
                     LDHELRTRGIRVSVIQPAYTKTPFDANLLEPDAKVDEYRGDRAGMVKRVAEVMATAEP
                     PSVVADTVLKAANAAKPQVRYTAGRLANRLRLLRKFAPAGVMDAGIRRDLRLAP"
     gene            6821..7366
                     /locus_tag="PK264_05570"
     CDS             6821..7366
                     /locus_tag="PK264_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD75395.1"
                     /translation="MRVSKEKAAENRERILKVAAQLIRERGIAGAGVDALTQAAGMTH
                     GSLYSQFGSKERLVEEALEYALAAGGEAASGSDTLKQSIANYLSADHRDQIGQGCPLA
                     ALACEMPRQSAGVRDKFTTGLRGMLKHLSSRMDAGLKSQQRDEQALAIVASLVGAVVL
                     ARAVNDQELSDAILRATKKAV"
     gene            complement(7442..8062)
                     /locus_tag="PK264_05575"
     CDS             complement(7442..8062)
                     /locus_tag="PK264_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD75396.1"
                     /translation="MPRPAEPTRQRILEAAYRLLRRQGYSRVTMDEIAAAARLTKRTL
                     YHHFESKDRLLADVLAAQHELALRAFRTFGDKLSGSPQEIVGGMFRELAVWADRPRWA
                     GSGFTRLVIELADLPGHPARKIARKHKIELENCLADLLAQSGVERSKELARAIWLLSE
                     GAISLILVHGDRGYSAAASDAATMLVQRYLSVQTANPFPPQFSPGR"
     gene            8161..9258
                     /locus_tag="PK264_05580"
     CDS             8161..9258
                     /locus_tag="PK264_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRD75397.1"
                     /translation="MSRPRLTFRGITARPVVLKLKRPVVARIATITEWPIILIDLTTE
                     EGIVGRSYLEPYVVKSMRYLVPALQDLGAMLKGRQVAPSELFEASRKSLHFVGYEGLS
                     MIAASGLDMAAWDALAKAANIPLCVMLGGTVGPVKAYNSNGLWLRAPEAVAEEAVELR
                     DEGGFAGLKLRLGRDRPADDLATIAAVRKAVGDEMRLMVDFNQGLNLAEALHRCHAID
                     SYDLEWIEEPIVYDNLDGLAQLTAELKTPLQIGENFYGPREMLKALQKKASDLVMPDF
                     MRIGGVSGWMRAAAIAGAAGIPMSTHLYPEVAAHVMRATETAHWLEWQDWADPVLQRP
                     YEMKGGQLHIPDVPGVGLDWNEDIVAANQLS"
     gene            complement(9510..>9784)
                     /locus_tag="PK264_05585"
     CDS             complement(9510..>9784)
                     /locus_tag="PK264_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75398.1"
                     /translation="FWLSTIPLTSGLVATFFGTTWMSMLFGFVFLSHQIGAFLGVWLG
                     GRLFDLTKSYDAMWWISIALGLSAALLNWPIQERPVARLKAEVAKA"
     assembly_gap    9785..9794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9795..10790)
                     /locus_tag="PK264_05590"
     CDS             complement(<9795..10790)
                     /locus_tag="PK264_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75399.1"
                     /translation="MTTSPAKSALPLWLLILIVGLIVGLSMGRTQSMGLYLPHVTNAL
                     SVGREPFGLAMALTQLTMGIGAPLSGGLIDKFGAGRIVAICVLAALGGLYLMYAATTA
                     VDLLLSGVLMGIGVSGTGMTSLVGTVGRLAPPEKRLSAIASIGMAAGIGGFVALPVMH
                     FLIEVTGWRQSLLWLMAITALLIPLAWPIGGKPQVQPGAIKVQTFQEALAEAFRHRGY
                     LLLTAGFFVCGFHVAFIMVHLPAFTIDQGLPTWVGPFALSVVGIANIVGTYLAGQSGR
                     FIEKRQGLTLIYIGRAVLFLGFLYLPMTPVTVIGICGLLGLLWLATIPLTSGLVAT"
     gene            complement(10920..11528)
                     /locus_tag="PK264_05595"
     CDS             complement(10920..11528)
                     /locus_tag="PK264_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HRD75400.1"
                     /translation="MTTSVEFHFDFGSPNAYLAHRVIPSITARTGVRFVYHPVLLGGI
                     FKLTNNQPPMVQFKGIRNKAEYARLETARFIKRHGIAAFRPNPHFPVNTVQIMRGAVL
                     AGRDGFFDLYVETAFRAMWEEGRKMDDPQVIKATLIDAGIDGDRVLEGIQEQSIKDKL
                     LADTEASVERGNFGSPTFFVDGEMYFGKDRLDDVERVIGKAG"
     gene            complement(11525..12211)
                     /locus_tag="PK264_05600"
     CDS             complement(11525..12211)
                     /locus_tag="PK264_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD75401.1"
                     /translation="MDKHANAPEHGLVVWGVGTARTIRVHWALAELGLSYETKPIGPR
                     TGETKTPEFTAITARQKIPVMQHGDTILTESLAIIAYLSDRFASSSNRLLPLDPLGRA
                     RCLEWCAFALSELDATSLYVIRRHRDLAATYGAAPAACEAAAAYFGQQMRSVDRALDD
                     GRHCVMGETFSAADILLSTCLTWATRYGVPISDTAQTYNDRMTRRPALAVASIRNTNP
                     SSPQSLGAGV"
     gene            complement(12223..13416)
                     /locus_tag="PK264_05605"
     CDS             complement(12223..13416)
                     /locus_tag="PK264_05605"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD75402.1"
                     /translation="MPGPLAGVRVIDLTTVISGPVCTMLLADQGADVIKVEAPTGDIG
                     RRTAGSTGISAMFASSNRGKRSIALDLKQPAALDIVRQLIAKADVLVQNFRPGTMERL
                     GLDEPALRRLNPRLITVSISGVGDSGPYVQKRVYDPIIQSLSGLADIQADQATGRPRM
                     VRTIIADKTTAIHAAQAVCAALFARERTGEGQHVRLSMLDTMVAFVWPEGMTQYTVVG
                     QEGATPSSTNRPDLIFETTDGYITVGSLSDDEWRGLCRVLDKPDWIDDPRFRTPALRS
                     TNATERLTLVGKILKTNRSGYWLERLDAADVPCAPVLRRADVMDNEQVVHNGLLEPVH
                     QPGLGLLRQPRPAARFERTPASIQGPAPRLGEHTDDVLREAGLSDDEISALHASGAAR
                     SWRPA"
     gene            complement(13466..14197)
                     /locus_tag="PK264_05610"
     CDS             complement(13466..14197)
                     /locus_tag="PK264_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75403.1"
                     /translation="MSISKAILVVGAGDATGGAIARRFAREGYTACVTRRTVEKLAPL
                     VARIEAEGGKARAFGSDARDEDQVVDLIATIEREVGPIEIVVFNVGGNVRFPIRETTS
                     RVYRKVWEMCAFGGFLVGREAAKVMVPRGRGTILFTGATASVRGGVGFSAFSGGKHAL
                     RALAQSMSRELGPEGIHVGHVVIDGGIDTAFTRERRGDAYDDVKAKGGILDPEHIAEN
                     YWNLHMQGRTAWTHELDLRPWVEKW"
     gene            complement(14318..>14677)
                     /locus_tag="PK264_05615"
     CDS             complement(14318..>14677)
                     /locus_tag="PK264_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD75404.1"
                     /translation="VTGLIGVIVAVVPDLWAGLFTTDPAVLAVSRTYFHWVGPCYGFF
                     GLGLCLYFASQGAGRVLGPVLTGTLRLFIVGVGGVWLASEGSGTSTLFAVIAAGMAVY
                     GISTAAAVAASRWGPMR"
     assembly_gap    14678..14704
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14705..15700)
                     /locus_tag="PK264_05620"
     CDS             complement(<14705..15700)
                     /locus_tag="PK264_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD75405.1"
                     /translation="MTATTAPACPAQANPLLAGAVLPTLLRLSLPNVAAMLAMALVAI
                     GETIYVGSLGTAALAGFALVFPLVMLQQMMSAGAMGGGVSSAIARAIGAGDDRRAAAI
                     ALHAAIIGTLAGLAFTAIMISWGAAIFRLLGGSGEALEQALAYANVAFLGSIGVWLTN
                     TFASVVRGSGNMRVPSTTLLLVNGAQVVLGGGLGLGLGVLPRLGMPGVALGQVIAYVG
                     GAAFLLAYLASGGARVRLALRGVTIERSIFADILRVGALSCISPIQTVMTSLIATRIV
                     ATGGAVALAGYGIGSRLEFLLVPITFGIGVASIPLIGMAIGSGDVARAREVAWTGG"
     gene            15906..16862
                     /locus_tag="PK264_05625"
     CDS             15906..16862
                     /locus_tag="PK264_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD75406.1"
                     /translation="MTKWNAKTRYRLPSLDGLATFEAAARTLSFTTSAGELSITQSAV
                     SKQVKQLEDQLGVALFERRPRALALTDAGYQFRQAAVDALDRLQAAVDLLQRGTKGTE
                     IAVTMTTTFAAMWLIPRLDRFTARHPDVDVRISATTTLLDLGRDRLDLAIRICAPHMA
                     PPDSPRLFHADVIAVCSPRLLKDPTRPLTSPKDIVNHTLLRCDLPELRGSFMDWSTWL
                     SALALGDLRPAATLTFNQADQAVQAAAAGQGIALAQARIVDEMVRSGVLVAPFGSGVP
                     GDRAFFVVRAPARNRSPARAAAVSAFIDWLIEEARSNDIDGQ"
     gene            complement(17458..17925)
                     /locus_tag="PK264_05630"
     CDS             complement(17458..17925)
                     /locus_tag="PK264_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003573327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HRD75407.1"
                     /translation="MPTYSTKRHVPHSAREMFDLVADVERYPEFLPLCEALKVKSRGG
                     SVEKPNLIADMTCGYGAIRETFTSRVTLERGAATPQILVEYLDGPFRRLVNQWRFVER
                     PGGSTVDFFIDYEFKSIVLQLLMGAMFDRAFQKFAEAFETRAVKVYGARPATG"
     gene            complement(17944..18516)
                     /locus_tag="PK264_05635"
     CDS             complement(17944..18516)
                     /locus_tag="PK264_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD75408.1"
                     /translation="MGAVEAVSPLQSTECRRILILGSSGAGKSTLADEIGRKLTLPVI
                     HLDKEFWLPGWVEPEKPVWRVKVAELVQRDAWVMDGNYSATLDIRLPRADAIIWLDLS
                     RRVCLSSAIWRTLASFGRTRPDMAPGCPERIDIPFFTDWIWNYPTRSRPGTLALIERE
                     RSLGERRTIVLGSRAEVRRFVAGLPSSLSA"
     gene            complement(18503..>18875)
                     /locus_tag="PK264_05640"
     CDS             complement(18503..>18875)
                     /locus_tag="PK264_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRD75409.1"
                     /translation="AIRPGARYFHSLRLLQRVKELDPAMFTKSGIMVGLGERREEVLQ
                     VMDDLRSADVDFLTIGQYLQPTRKHAEVKEFVTPEAFAGYERTARAKGFLLVSASPLT
                     RSSYHADADFAKLKAARLGRG"
     assembly_gap    18876..18962
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18963..19467)
                     /locus_tag="PK264_05645"
     CDS             complement(<18963..19467)
                     /locus_tag="PK264_05645"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRD75410.1"
                     /translation="MVTVVDTTRKSEPERPRHPEKQARPDTALLRKPDWIRVKAPTSP
                     GYSETKRIVREHGLHTVCEEAGCPNIGECWTKRHATMMIMGSICTRACSFCNVATGLP
                     KPLDPAEPENVATAVEKLGLGHVVITSVDRDDLADGGAAHFASVITAIRARSPRVTIE
                     VLTPDFLR"
     gene            19586..20983
                     /locus_tag="PK264_05650"
     CDS             19586..20983
                     /locus_tag="PK264_05650"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRD75411.1"
                     /translation="MTDHLKDVLKTLDANEEKAVERLFELLRIDSISTDPAYKGKCQK
                     AAEWCRDQLAEIGFASAKVVPTTGHPMVVGHDRRSVPKGMPHVLFYGHYDVQPADPID
                     LWTSPPFEPRIATEKGNGKVIVARGAEDNKGQLMTFFEAARAWKKVAGELPVAVTVLL
                     EGEEECGSPSLPGFLKTHAKEVSCDLALVCDTGQWDKGTPAITTMLRGMAATEITVKC
                     PSRDLHSGMYGGPAMNPIRALARIVASLHDDKGRVAVPGFYDGIVKPTRKQLAQWESL
                     GFDDKAFLGAVGLKTPAGEQKCSVLEQLWARPTCEFNGITGGYQGVGTKTVIPSEASV
                     KITCRLVPGQDPKKVLAALKAHVLAAAPADSEVNFAYLRGSPAIAFDTEASHIRAAAE
                     GLAEEWGKPAVMMGCGGSIPIVGSFKDVLGMDSLLVGFGLEDDRIHSPNEKYNLSSYR
                     KGARSWARILAKLAR"
     gene            21053..21127
                     /locus_tag="PK264_05655"
     tRNA            21053..21127
                     /locus_tag="PK264_05655"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21085..21087,aa:Lys,seq:ttt)
     gene            complement(21186..>22394)
                     /locus_tag="PK264_05660"
     CDS             complement(21186..>22394)
                     /locus_tag="PK264_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD75412.1"
                     /translation="GGLSTGGLVIWIDRMSDWTGRQVIAGIAAELMDRLPKDAIQGPP
                     RADWGSRDAATAAYWAQRTAAYHGIVTWSPTIDPEALKSLSLRMVLEEKARLLLHSWA
                     SMPIVEDGALRGAIFETKEGRQAVLAKVVVDTTGDADLLARAGAPLESDIDTGDIHHC
                     INTAFLMGGVDMDRFIAFRTTEKARYSDLLQQGREELRSFEKPFVGWRRDVALFMGPR
                     LTGYSAVNVEDLTEVEIRSRELTDHHITFYRRNMPGFERAFLMLGGPQIGVRHSRRIG
                     GVRKVVRAQWDTGEVWPDEIGVSTSLSPKFPNISVPYGALVPAILDGVIAAGRHVACD
                     ATSHSFLREIPQCWMTGQAAGIAAALAVARSQAPRHVAPRLIQQELLRQGAYLSPTTE
                     TSARVSAAAE"
BASE COUNT         4241 a         6986 c         7252 g         3791 t
ORIGIN      
        1 gattgagcga gaagctcttc gtgctcggaa atggtgatcg gacctgactg cgggctgccg
       61 atccgcgcgc ggaaacgctt gatggcgtcg tcgagctcgc cgcgcggcac ctggcccgcc
      121 tccgcattga ggttggcagc ccagatcagc gcctcgtgca tctcgaggag cgtcccgtgc
      181 ggcaaccacg ctggagcatt gcgctgcgcc gatgcggccg ctgccgtcag cgcaagaagg
      241 agacccgctg caatcaggcg gacgacggct cgcaaacgat cccgggaaaa agtcatgaga
      301 ggtccctcgc aatgatgagc acgccgagtg cagcacgcgg agcggccggc ggcaactaag
      361 agataacgac gaagcagacg gcgatgacca ggtcacgtga gcgtgcgagg ccaagggcga
      421 cccgatcagg tcgagcggca gggggcgtgg gctcgccgct ctgtcagccg cccggacgcg
      481 gacgtgccga catgcctcag aaactgctgg tcactaacgc gcgatcgcgg acctggtcgg
      541 gcccggaagc tcgtaccaac ggctgcaagt ggcaacggtt caccggccaa cgtggctttg
      601 gaattcagcc gctcgctggc tgcctggctc gcaaaaccag cgctccaggc cccctcagca
      661 gcgagccgag aacaaaacaa aaacattact cgacagcagc gcggactcac ggtatgttct
      721 atttaagttc caagcccaaa tccaagcgac ccccagcagg tgacccatgg ccgagctcct
      781 cattgccctc gatcttgccg acccgcatca gggcaaggct tgcattgtcg acagcctcat
      841 gcggctgggc gaggcttggg cgcgcccact tgaaacggtg tgggtcgtga ggaccgaacg
      901 ctcggcggcc gctgtagagg ccgagctcag ccaatgtctc ggtccggatg acggcctgct
      961 cgtgcagccg attgccgagc agggtgccct cgtgaacgcc agcctccgat ggtttcgaag
     1021 gcgggtcgac gtcgacgcag cagagcgcgc cgagcgggca accgggcatg cgcctggacg
     1081 ggaaggcggc gtccgcgcgc cgtccaacgt cgtgacgctc cgcgtcgcgc cgagcccgtg
     1141 gctgcaggtt gcccgcgaga atgccgtcag ggccgccagc tgagaaagtt ggtgatcagc
     1201 ccgaggccca ggcgctggct cttttcaggg tgaaattgcg tgccggcgat gttgtcacgg
     1261 ccgacgatcg cggtgacggg accaccatat tcggtctcgg cgaccaaggt gccggccggt
     1321 gcgttggtga ggtgatagga gtggacgaag taggcgtgca agccctgctc gccggtggcc
     1381 aggttggcaa ggagcgcgtg cgggcgctcg gggcggattg tgttccagcc catatgcggg
     1441 atcttgaggc cgggatctgc tgggcggatg aggtcgacgt cgccgccgat ccagctgaga
     1501 ccagggtgga cgccgtgctc gagcccgcgg gctgctagaa gctgcatacc gacacagatg
     1561 ccgagaaatg gtcgtccggc ctcgacgaca ttgcgctgca gtgcctcgac catgcccggc
     1621 acagcgtcga gaccgcggcg gcagtcggca aaggcgccga caccggggag gacgatccgg
     1681 tccgcgccgg caacggccac tgggtcgctc gtcacctcga tgcgggtcgg tcgggcgcaa
     1741 tcgcgtgccg cgcgctcgaa ggccttggcg gcggagtgga gattgccgga cccgtagtcg
     1801 acaatggcga tacgctgcat ggaacctcag cgcctccagc cggcaagagt ggtcgcgcgg
     1861 ccagcccgct cggctccgac agtcgttgcg aacgaggcgg cggcggcgcc gagtgggcgg
     1921 gtcggaagcg ggcctgacgg ctgctcgggc agccagacat cgaggaaacg gcgctcgcac
     1981 tcatcgacgg tacggccgac gaccgatccg atctgggtgt agccattgcg atcgagcgtc
     2041 cagcggcgaa gtgtgtccgc ctcgaggccg atgatgaggt gcatcgcgac tgtcaggatc
     2101 tgccatatgc gcggatcggc gtccgtggcg ctgaggccca ggcggagtcc accgacgatg
     2161 acgatataag cgatgagcac gagccagagg cgctgcacga ccagccagag cggcgtgagg
     2221 gctgctgcga gccagtcgaa tccttccttg acgaactcga gcttctcggc gcgctcgagc
     2281 ctatctcccg gcgggtttgg tctttcgtga accgtatagg tctgcaaggg cggctccatc
     2341 gcggggtggt gtccatccgg gatcgccccg cccttctact gcgtcgccgg tgtgccgcca
     2401 aggctgctca agggtgccgc tcgcgtgcct ttgcgctgcc ttgatccttg cgctattctc
     2461 catcgtatga gcattctcgg ttcgacatgt gcagcctcgg gctgcgttcc tcgggcgcga
     2521 cgcgccgtag tgctgccgat cgccgtgctt ttcgtgctgg cacttgccgg gcctgcggcg
     2581 agccagtcca ccggtgagcc gcctcggtcc gagcagccga agaaatccga gcccccgccg
     2641 ggcaaggggg cacccgcaac gccgccgacc ggcaagggag ccaccgcgcc gaatgccgcc
     2701 gatccgttga agctcaacca gatccccgag acgtccgagg ccaaggtgaa gctgctgcgc
     2761 gagctttacg cgctgctggc gacggccgag gacgccgaga gtgcgaaccg aatttcctcg
     2821 cgcatcgagc ggctgtggtc gcactcgggg agcgacacgg tcacgctgct gatggagcgg
     2881 gccaacgccg cgatgagcaa caagaaggcc gagctggcac tcgagttgct cgaccgcgcc
     2941 gtgaagctcg cgcccgacta tcccgaggct ttcaaccggc gggccttcgt ccatttcact
     3001 gagaacaatt tcgcaggcgc ggtcggtgat ctgcgccggg ctctggcgct cgatcccaat
     3061 cactacaagg cgatggacgg cctcgtgcag atctggcgcg aggtgggtaa caagcgcggc
     3121 gcctattacg tgctgaaaca gctcgtcgac gtgcacccct tctgggcggg cgccaagcag
     3181 gtgctcgagg aactgaagaa ggaggtcgag ggtcaggaca tctgagtcct cggcggcgcg
     3241 gattgcgctg acggttcccg cggagacatt ttcatgcgca atccgatcga gttcggccgg
     3301 cgttcgacgc cgagagcccg gacgctcgcc atggccggcg ccgccgtggg cgttgccggt
     3361 ggcgctttca tggccttcgg cgacagtctc gagcgctact cgggcgtcga tcttgcggtg
     3421 ccaagctgcg ccgagccagc ggccgtcagc gatctgcgtc gcatcatgga cgactactac
     3481 gccaagaagc cggagcgcgt gaaggtgcgc gcgatcgagg cgccaaagct cacgcggcat
     3541 tcctcgaccg aggcgcactg cgagggtcgc gtgcgcctca ccgacggcaa ggagtacgcc
     3601 ctgcgctacc ggctctacaa ggcggagcgg cagctgcgca tccagttctc agtgagcgac
     3661 agctgaggtc gatcacctgg ggctcacgcc gttgtgatga ggcgggtcgg cgccgcttgc
     3721 gtttaatgcg gcaacagcgc tcggccgaca gcgaattgcg ggcgcgacca ctcgaggagt
     3781 gaccagcgat gtcagcatct accttgtctc gccgcgccat gcttgcaacc actggtgcgg
     3841 gcgtgatggc agccatgtcg ggcgtccccg cgcaggcgct cgacgtgccg aagcctggtg
     3901 cagccgctcc agagttcacg ctgaatgaca gcacggggaa gcccgtgacg ctcgcgagct
     3961 tcaagggcac gcccgtgatc ctcgagtgga cgaaccacga gtgcccgtac gtgcgcaagc
     4021 actatggtgc cggcaacatg caggagctgc agcgcgaggc ggcggccaag ggcttcgcgt
     4081 ggctgacggt gatttcctca gcacccggta cgcagggcca tgtcgacggc ctcgaggccg
     4141 accagctgac cgctacccgc aaggcggcgc cgaaggccgt gctgttcgat ccgacgggcg
     4201 tggtcggccg ccgctacggc gcgacggcga cgccgaacat gtacgtgatc gataaggccg
     4261 gcatgctcat ctacatgggc gccatcgacg acaagccgtc gaccaacccc gccgacatca
     4321 agggcgccaa caactacgtg cgcgccgcca tgaccgccgt cctcgccggc agcactcccg
     4381 ccgtgcgctc gacccgcgct tacggctgct cgatcaagta cgcgcccgtg aggagttgag
     4441 cgggtccgat acaaggtaat gcatgcaccg gcccggagca cctccgggcc gtcttgcgtt
     4501 ggcgcgtcgg ccgtgagttg gcggcttggg gccaggtgca gaccaacgaa gacacgaatg
     4561 atctgaccgc gtgtgctttt cttgctcgcc aaccgagatg ctcgtgtgcc aagttttgcg
     4621 gcgatgcaac tggattgtcg ctttcatggc ggcaaacggt cagtacaggg cgatcaccga
     4681 ccatcgtcgc ataggtcgac ttcaacccgt cgccgggcga ggcggatccc aagccgaaga
     4741 agtgcacgcg ctcaacacag gtgttgcaga ttgtccttga aaccgtcgtg cgacaccgct
     4801 gagcatcatg agatagcaac tatagcagcc tgaccacgac cttgcccttg gctcgcccct
     4861 gttcgagata tgcaagcgct tccttggcct gctcgaacgg aaagattcga tcgataaccg
     4921 gcttgatgcg ccccgcatcg agcagcgcgc cgatctctgc aagctgaccg ccgtccggat
     4981 gcacgaacag gaatgagtat gtggcgcctt gcttcctggc gaggcgattg atcttgcggc
     5041 tcaggacgcc gaataacgcc ttcaagacaa agttcatgcc tcgatttcca gcgaaggccg
     5101 catcaggtgg cccgacgagc gagacgatgg tgctcttcgg cttcagaata cgtaccgact
     5161 ttttgagcgc atcgcctctg accgtgccga ggactgcatc gtagtcgcgg agtacgttct
     5221 cgaactgttg ctttttatag tcgaccactt cgtcggcgcc gagactgcgt accagatcga
     5281 cattggcggt gctggtcgtc gttccaacct ttccgccgag gtgcttcgct aactggattg
     5341 cgaacgtacc aatgccgcct gatccggccg gaataaatac ccgctggccg gacttgaggt
     5401 tcgcacgcac ctttagcgct tgccatgacg tcaagccgac catcgggatg gatgccgctt
     5461 ggacaaagtc gagggtggca ggcttgggcg ccgccgcact ttccgggacg acagcaaatt
     5521 cggcgagaga accgagtccg gtgtcgaaga tactcgcgta aacggcatcg cctggcttga
     5581 agcgtgtgac atggctgccg acgtcgatca cgacgcccgc cagatcactg cccagcgtag
     5641 ccggcaggtc gaatttcaag aatggcttga aggtgccttt tgggatcatg agatcgacgg
     5701 gattcagtcc gaccgcatga acttgtacga ggatctcatc tggcttcgga gttggccgag
     5761 gaatatccgc aaacgcgatg ttgtcacgct tggcatatcg gtcaaaggtg agggctcgca
     5821 tattttcaat ccaaattgac gttgaagtcc gcacggctgg ttgttcgcga cctatggcgc
     5881 aagccgcaga tcccgccgga tgccggcgtc cattacgcca gcgggcgcaa atttgcggag
     5941 aagtcgcaga cggttcgcaa gacgaccggc cgtgtatcgt acctgcggtt tggccgcgtt
     6001 agccgccttc aacacagtgt cggctacgac gctcggcggc tcggcagtcg ccataacctc
     6061 agccactctc ttgaccattc cggcccgatc tccccgatac tcgtcgacct tggcgtcagg
     6121 ttccaacaag ttcgcatcaa acggcgtctt cgtgtacgcg ggctgaatga ctgagacccg
     6181 gatgcccctg gtgcgaagct cgtggtcgag tgactccgaa tagccttcca cggcgtgttt
     6241 ggtcgccgaa tagagcgcgc catatggcat cggcagaaag ccgagcacgg agccgatatt
     6301 gatgatgcgt ccgctggcct gcttgcgcat gtgcggcacc acggcgcgcg tcatccggac
     6361 caggccaaag aaattagttt cgaagatcgc gcgggcctgt tcaagagaac tctcttcagc
     6421 tccggcaggt gcgatgccga agccagcgtt gttcacgagc agatcgatgc ggccatcgcg
     6481 cagaatcacg tcgccgacga gcgccatgac ggactcatcg ctggtcacat cgaggggcag
     6541 catctcgaaa ggcggccgac ctgacttcgc tccacgtcgg ctggtgccgt aaaccttgta
     6601 acccgccgta gcgaggcgcg cggcagtggc ctcgccgatc cccgatgaag ccccggttac
     6661 gatcgcaatt ttgctgttgg ctttggtcac gtacgtctcc tgggtgtgtg gccggtcgta
     6721 gttgcatcgt ggattactta ttcattacga ccgcaatgat tgacatagat gacgagcgtc
     6781 actataaaag tcaagagcca atttcaggag gacttgcggc atgcgcgtct cgaaggagaa
     6841 ggcagctgaa aatcgcgagc ggattctcaa agtcgcggcg caactcattc gcgagcgcgg
     6901 catcgcaggg gccggtgtgg acgcactgac gcaagctgcc gggatgaccc acggtagcct
     6961 ttacagtcag ttcggctcga aggagcggct tgtcgaagag gctttggaat atgccctagc
     7021 tgcaggtggc gaggctgcat ctgggagcga tacgctgaag cagtccattg cgaactacct
     7081 gtcagccgat caccgggatc agatcggtca aggctgtccc ctcgcggctt tggcctgcga
     7141 gatgccacga cagagtgccg gcgtgagaga caagttcacc accgggttgc gaggcatgct
     7201 caagcacctg agcagtagaa tggatgccgg gctgaagtcg caacagcgtg atgaacaggc
     7261 gttggcaatc gttgcgtcgc tggtcggagc ggtcgtgctt gcgagagccg tcaatgatca
     7321 ggagctgtct gatgctatcc tgcgcgccac gaaaaaggcg gtttgacgcc tgatcgaaac
     7381 gccaacagag cacctacgtc cgcacatgaa gttcggtacc caccagaatt tgccgacacg
     7441 gtcagcgccc cggtgagaat tgagggggga acggattagc tgtctgtacg ctcaagtacc
     7501 gctgcaccag catggtcgcg gcatcagacg cagcagctga gtagccgcgg tcgccgtgaa
     7561 caaggatgag cgagattgcg ccctctgaca ggagccaaat ggcacgagcg agttccttcg
     7621 accgttcgac tcccgactgc gcgaggagat cggccaagca attctcgagc tcgattttgt
     7681 gcttgcgcgc aatcttacgc gctggatggc caggcaagtc ggccagctct atgacgaggc
     7741 gagtgaagcc ggagcctgcc catcgtggcc tgtcggccca aacggccagc tctcggaaca
     7801 tccccccgac gatctcttgc ggagaacccg acagcttgtc gccgaacgtc cggaaggcgc
     7861 ggagggccag ctcgtgctgc gcagcaagaa cgtcggccag cagtcgatcc ttgctttcga
     7921 agtggtggta gagcgtgcgc ttcgtcagtc gcgcggccgc cgcgatctcg tccatcgtca
     7981 cccggctgta gccttgccgg cggagcagcc gataggccgc ttcgagaata cgctggcgcg
     8041 tcggctcggc gggtcgaggc atccggtgtc cctctcaaag tatactcaat agtgagttta
     8101 cacgcgcgag gaccccgctt caattctggg aggcatcaga caaatttgga aacatagccc
     8161 atgtcacgtc cccgattgac atttcgaggc ataacagcgc ggcccgtcgt cctgaagctg
     8221 aagcggcccg tcgtcgctcg cattgcgacc atcaccgagt ggcccatcat actgatcgac
     8281 ctcacgacag aggaaggcat cgtgggtcgc agctacctag agccttacgt cgtaaagtcg
     8341 atgcgatatc tggtgccggc actgcaggat cttggcgcca tgctgaaggg ccgtcaggtc
     8401 gccccctcgg agctgttcga agcctcacgc aaatcgctgc acttcgtcgg ctacgagggc
     8461 ttatcgatga tcgccgcgtc cggcctcgac atggcggcct gggatgcgct ggccaaggcc
     8521 gccaacatac cgctctgcgt catgctgggc ggcacggttg gaccagtgaa ggcctacaac
     8581 agcaacgggc tttggctgcg agcaccggaa gccgttgccg aggaggcggt cgagttgcgc
     8641 gacgaaggcg gcttcgccgg cctgaagctg cgtctgggtc gcgataggcc cgccgacgac
     8701 ctcgcgacga tcgcggccgt caggaaggcc gttggcgacg aaatgcgcct gatggttgac
     8761 ttcaaccaag ggctcaacct cgcggaggcc ttgcatcgat gccacgcgat cgactcctat
     8821 gatctcgaat ggatcgaaga gccgatcgtc tatgacaatc tggacggcct tgcgcagctc
     8881 acggcggagc tgaagacgcc gcttcagatc ggcgagaact tctatggccc gcgcgagatg
     8941 ctgaaagcct tgcagaagaa ggcaagcgat ctggtaatgc cggacttcat gcgcatcggt
     9001 ggcgtatccg gttggatgcg cgcagcagca atcgcggggg ctgcgggtat cccgatgtcg
     9061 acccatctct atccggaggt ggcggcacac gtcatgcggg cgaccgagac agcgcactgg
     9121 ctcgaatggc aggactgggc agacccggtg ctgcagcgac cctacgagat gaagggcggg
     9181 cagctacaca ttccggacgt ccccggtgtc ggcctcgatt ggaacgagga catcgttgcg
     9241 gcgaaccagt tgagctgagc cgtccagcgt tcgcaacgga tcgggacgct gctgttagtc
     9301 agagcggagg cgccggcaaa gtccggcccg aaggacgcaa ggccgtgacg atcaatgtcg
     9361 agccctcgag tcatggttct cgcttcgagc cgttgatggt cgtcaacgat gggaccaatg
     9421 tgaaatgcgt ggccgccgat ccgagctcgg tgcatccgcc agacgaagcg agccgagacc
     9481 tccaacaagt ctcggcgctc gcaggcggct tacgccttcg ctacctccgc tttcaagcga
     9541 gcgaccggcc tttcctggat cggccagttc agcagagcgg ccgacagtcc gagcgcgatc
     9601 gagatccacc acatagcgtc gtaggacttg gtaagatcga atagcctgcc gccgagccag
     9661 acaccgagaa acgcaccgat ctgatgcgac agaaacacaa acccgaacag catcgacatc
     9721 caggtcgtgc cgaagaaggt cgccacgagg ccgctcgtga gcgggatggt cgacaaccag
     9781 aacannnnnn nnnncgtcgc gacgagacca ctggtcaaag gtatcgtcgc gagccagaga
     9841 agccccagca ggccacagat gccaatgacc gttacaggcg tcatcgggag gtacaggaaa
     9901 ccgaggaaca ggacggcacg accgatgtaa atcagggtca agccctgccg cttttcgatg
     9961 aaccgccctg attgcccagc aaggtatgtg cctacgatat tggcgatccc gacgacggag
    10021 agtgcgaagg gaccaaccca agtcggtagc ccctgatcga tggtgaatgc tggcaagtgc
    10081 accatgatga aggcgacatg gaacccgcaa acgaagaacc ccgcggtcag cagcaagtac
    10141 ccccgatgcc ggaacgcctc ggcgagtgct tcttggaacg tctgaacctt gatcgctcct
    10201 ggctgcacct gaggcttgcc accgataggc caagccaagg gaatgagcag cgcggtaatc
    10261 gccatcagcc agaggaggct ttgccgccag cccgtcacct cgatcagaaa atgcatcacc
    10321 ggcaacgcga cgaagccgcc aatgccggct gccataccga tggatgcgat cgcgctcaag
    10381 cgcttctccg gcggcgccag ccgtccgacc gtaccaacca gcgatgtcat accggtgcca
    10441 ctgacgccga tccccatgag gacgccacta agcaaaaggt ccactgcggt cgtcgccgcg
    10501 tacatgaggt acagaccacc gagagctgcg agaacgcaga tggcgacgat gcggccggct
    10561 ccaaatttgt caatcaaacc gcccgatagg ggcgccccga tccccatcgt cagttgggtc
    10621 agcgccatag cgagaccgaa cggctcgcgg ccaacactca gcgcgttcgt gacgtggggc
    10681 aggtacagac ccattgattg agtgcgcccc atcgacaagc caacgatgag gccaacgatc
    10741 aggatcagga gccaaagcgg taatgccgac ttcgcgggag acgtggtcat gaggtttctc
    10801 ggcccaaacg gtatgtcact ttcatgccat cgctggcacg accagccgca atgtcacatt
    10861 tgcttgctct ggggcggcat ggaactggag gcaccgatta ttctgctccg tggaattacc
    10921 tatcctgctt tgccgatgac ccgctcgaca tcatcgagtc gatccttgcc gaaatacatt
    10981 tcgccatcga cgaagaacgt cggactcccg aagttaccgc gctcgacgga ggcttccgta
    11041 tcggccaaga gcttgtcctt gatcgattgc tcttgaatgc cctccagcac gcggtcgcca
    11101 tcgatccccg cgtctatcag cgtcgccttg atcacttgtg gatcgtccat cttccggcct
    11161 tcttcccaca tcgcgcggaa agctgtctcg acgtagagat cgaagaagcc gtcacgccct
    11221 gcaagcacgg caccgcgcat gatctggacg gtattgacag ggaagtgagg attgggtcga
    11281 aaggccgcaa tgccgtgtcg cttgatgaag cgcgcggtct cgagccgcgc atattccgcc
    11341 ttgttccgga tacccttgaa ctgcaccatc gggggttggt tgttcgtcag cttaaagatt
    11401 ccaccgagga gcaccgggtg gtagacgaac cgaacccccg tccgcgcggt gatcgatgga
    11461 atcacgcggt gtgcgaggta ggcgttcgga ctgccaaaat cgaaatgaaa ctccacgctg
    11521 gtcgtcatac accggctcct agagactgag gtgatgaggg attggtattt cggatgcttg
    11581 ccacggcaag agccgggcga cgcgtcatac ggtcgttgta cgtctgagcc gtatcggaga
    11641 tgggcacgcc atagcgcgtg gcccacgtga ggcacgtcga caataaaatg tccgcggccg
    11701 agaacgtctc gcccatgacg cagtgccgtc cgtcatcgag tgcccggtca accgagcgca
    11761 tctgctggcc gaagtacgcg gcggctgcct cgcaagcggc gggcgcggct ccataggtcg
    11821 ctgccagatc gcggtgacgg cggatcacat agagcgatgt cgcatcaagc tcggagagcg
    11881 cgaaggcgca ccattcgagg cagcgcgcgc gcccgagcgg atcaagtggc aggagacgat
    11941 tgctgctact ggcgaagcgg tccgagagat aggcaatgat ggccaggctt tccgtaagga
    12001 ttgtatcgcc gtgctgcatt acggggatct tctggcgcgc cgtgatcgca gtaaattctg
    12061 gcgttttcgt ctcacctgtg cggggtccaa tgggtttggt ttcgtagctc aagccgagct
    12121 cggcgagcgc ccagtgcacg cgaatggtgc gcgccgttcc aacgccccag acgacgagac
    12181 cgtgctcggg cgcgttcgcg tgcttgtcca tggccgtccc cctcacgccg gtcgccagct
    12241 ccgcgctgca ccagacgcat gtagcgctga aatctcatcg tcggacagtc ccgcttcgcg
    12301 caggacatcg tccgtgtgct cgccgagcct cggtgccggt ccttggatgc tcgcgggcgt
    12361 ccgctcgaac cgtgcggctg gccttggctg acgcaagaga ccgagccctg gttggtgcac
    12421 gggctccaag aggccgttat gcacgacctg ctcgttgtcc atcacgtccg cgcggcgcag
    12481 gaccggagcg cacggaacat ccgcggcgtc gaggcgctca agccagtagc cgctccgatt
    12541 tgtctttaag atcttgccga ccagcgtcag ccgctcggtc gcattggtag accggagcgc
    12601 gggcgtgcga aaacgtggat cgtcgatcca atccggctta tcgagcacgc ggcagaggcc
    12661 ccgccattca tcgtctgaga gcgaaccgac cgtgatgtag ccgtcggtcg tctcaaagat
    12721 caggtccggc cgattggttg aacttggggt cgctccctcc tgaccgacga ccgtgtattg
    12781 cgtcatgcct tcgggccaga cgaacgcgac catcgtgtcg agcattgaaa ggcgcacatg
    12841 ctggccctcg ccggtacgtt cgcgtgcgaa gagtgcagcg cagaccgcct gcgcggcatg
    12901 gatcgcggtc gtcttgtcgg cgatgatggt gcgcaccatg cgcggacgtc cggtcgcctg
    12961 atccgcctgg atgtcggcga gtcccgagag cgactggatg atcggatcgt aaacgcgctt
    13021 ctggacgtag gggccggagt cgccaacacc gctgatcgag actgtaatga gacgcgggtt
    13081 aagacgccgg agagcaggtt cgtcgaggcc gagccgctcc atggtgccag gccggaaatt
    13141 ctgcacgaga acatcggcct tggcgatgag ctggcgcacg atatcgagcg cggccggctg
    13201 tttcagatcg agcgcgattg atcgcttgcc acggttcgac gacgcgaaca tcgcggagat
    13261 gcccgtgctg ccggccgtgc gcctcccgat gtcgcctgtg ggagcctcga ccttgattac
    13321 atcggcaccc tggtcggcca gcagcatggt gcagacgggg ccagaaatga ccgtcgtgag
    13381 gtcgatgacg cggacaccgg caagaggacc tggcatctgt gcaacctttc atcgagcgat
    13441 gcgaacgaaa cggaggacca tcgcgtcacc acttctcgac ccagggacgt agatcgagtt
    13501 cgtgtgtcca ggctgtgcgg ccctgcatgt gcaggttcca gtagttctcc gcgatgtgct
    13561 cgggatcgag gatgccaccc ttcgccttga cgtcatcgta ggcatcgccg cgccgctcgc
    13621 gcgtgaaggc cgtgtcgatg ccgccgtcaa tgacgacgtg cccaacgtga atgccttctg
    13681 ggccaagctc gcgggacatg ctctgggcga gtgcccggag cgcatgcttg ccgccggaga
    13741 atgcactaaa gccgacgcct ccgcgcacac ttgccgtggc gccggtaaac aggatcgtgc
    13801 cacgcccgcg cgggaccatc actttggctg cctcgcggcc gaccagaaag ccgccgaacg
    13861 cgcacatctc ccacaccttg cggtagacgc gtgatgtcgt ctcgcggatt ggaaaccgca
    13921 cgttgccgcc gacgttgaaa acgacgatct ctatcggacc gacctcacgc tcgatcgtgg
    13981 cgatgagatc gacgacctgg tcttcgtcgc gcgcatcgct cccgaacgcc cgggctttgc
    14041 caccttcggc ttcaattcgc gccacaagcg gcgcaagctt ctcgaccgtg cgccgcgtca
    14101 cgcacgctgt gtagccttcg cgggcgaaac gacgcgcgat ggcgccgccg gtcgcgtctc
    14161 ccgcaccaac gacaaggatg gctttggaaa tggacatctc atgacctcca cacgtgctgg
    14221 aaacgtcgaa gcggggattg tcacagtctc gaagagtgat tgaagccgcc cccggtgtgg
    14281 gacgagtatc tcgtcggaaa cacgaatggc ctctggttca tcgcatcgga ccccaacggc
    14341 ttgccgcgac ggctgcggca gtactgatgc catagacagc cattccggcc gcgatcacgg
    14401 cgaagagtgt cgatgtcccg ctgccctcgc ttgcaagcca gacgccgccg acaccaacga
    14461 tgaagagacg cagggtaccg gtgagcaccg gaccgagtac ccggcccgcg ccttgcgaag
    14521 cgaagtagag acaaaggccc agcccgaaaa agccatagca ggggccgacc cagtggaaat
    14581 aggttcgcga gacggccagc acagccggat cggtggtaaa gagcccagcc cacagatcgg
    14641 gcacgaccgc cacgatcacc ccgatcagac cggtgacnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnncccgcc ggtccaggcg acttcgcgtg cccgtgcgac gtcgccgctg ccgatcgcca
    14761 tcccgatcag cggtatggag gcgacaccga taccgaaggt tatcggcacc agcaggaact
    14821 cgagccgcga gccgatgcca tagccggcca gcgccaccgc cccgcctgtg gccacgatgc
    14881 gcgtagcaat caacgacgtc attacagtct gaattggcga gatgcatgac agtgcgccga
    14941 cgcggaggat atcggcgaag atcgaccgtt cgattgtaac gccacggagc gccagccgga
    15001 cccgtgcgcc gcccgaagcg aggtacgcga gcaggaacgc cgcgccgccg acgtaggcga
    15061 tcacttggcc gagcgccaca cccggcatgc cgagccgcgg caggacgccg agaccgaggc
    15121 cgagccctcc accgaggacg acctgcgcgc cgttgaccag cagtaatgtc gtcgacggca
    15181 cgcgcatgtt gccgctgccg cgaacgacgg acgcgaatgt gttcgtgagc catacgccga
    15241 tcgagccgag aaacgcgacg ttggcgtagg cgagcgcctg ctccaaggct tcgcccgagc
    15301 cgccgagcag gcggaagatg gcggcgcccc aactgatcat gatcgccgtg aaagcgaggc
    15361 ccgctagagt gccgatgatg gccgcatgca gcgcaatcgc cgcggctcgc cgatcgtcac
    15421 cggcaccaat cgcacgcgcg atcgccgagg agacgccgcc ccccatggca cccgccgaca
    15481 tcatctgctg cagcatgacg aggggaaaga cgagggcgaa gccggcaagc gccgcggtgc
    15541 cgagactgcc cacgtagatc gtctcgccga ttgcgacgag cgccatggcc agcatggcag
    15601 ccacattcgg gagactgagg cgcagcagcg tcgggagtac ggcgccagca agcagcggat
    15661 tggcttgcgc agggcaggcg ggagcggttg ttgcggtcat gttgaagtca tctgattggc
    15721 tgtctgggag ggagcacggg tcattccggc gggtctctgc atggtgaacc ccgacttctg
    15781 aacagtagcc gtcgcttgcc attccgtcat gaatggcacg accgatcgaa ttgcgttgcc
    15841 gcatgaactc acggtaacct catgtctcga ctgaacagaa gcaaacgaat gtttggcatc
    15901 gacgcatgac gaaatggaat gcgaagacgc gttatcgcct gccatcgctc gatgggctcg
    15961 cgaccttcga ggccgcggca cgcacactca gtttcaccac gtctgcgggc gaactctcca
    16021 tcacccagtc ggccgtcagc aagcaggtca aacagcttga ggaccagctc ggcgtggcac
    16081 tcttcgagcg gcggccacgt gcattggcct tgaccgatgc ggggtatcag ttccggcaag
    16141 cggcggttga cgccctggat cgcctgcagg cggcagtcga cctattgcag cgcggaacaa
    16201 agggaaccga gatagccgtc acgatgacga ccaccttcgc cgccatgtgg ctcatccccc
    16261 gcttggatag attcacggcc cggcacccgg atgtcgatgt ccggatctcg gcaacgacga
    16321 cattgctcga tcttggccgt gatcgactcg acctcgcgat ccgcatctgc gcaccgcaca
    16381 tggcgccgcc ggacagtccg cgactgttcc atgccgatgt catcgcggtg tgcagtccgc
    16441 gcttgctcaa ggatccgaca cggccgctga cgagtccgaa ggacatcgtc aatcacacgc
    16501 tgctccgctg cgaccttcct gagttgcgag gctcgttcat ggactggagc acatggctaa
    16561 gcgcactcgc gctcggggat ctgaggccgg cagcaactct gacattcaat caggccgacc
    16621 aggcggtaca ggccgccgcc gcaggacaag gcatcgcgct cgcccaagcc cggatcgtcg
    16681 acgaaatggt cagaagcggt gtgctcgttg ccccgttcgg ttcgggtgtc cccggcgatc
    16741 gcgcattctt cgtcgtacgt gctccggccc gcaatcggag ccccgcgcgg gctgccgccg
    16801 tctctgcatt catagactgg ctgatcgagg aggcgcggtc gaatgatatc gatggccagt
    16861 aaattggggc aagcagctcg atccccgccg attgaatggc gaggactgcg cacgcagctg
    16921 gtctcgtctg cctgcctaca gcacctgaca gtctagcagc gatggccatt ctgccgggcc
    16981 agattgagcg catagaaagc acacgttccc tgatcttcag tatctcaaga cggcggcaat
    17041 tggccgtgcg cctcaagggc gcgaactatc agtgaggaag gatctcacac agacaattgt
    17101 cgattgacct gggagcaaga gagcggctcg gtcggtcaac tgatcgggcc ggagcctcgc
    17161 gcaaaggacg gccttgggcc gtctggcgat ccgacgggcc gtaacgtatg cgaagcccgg
    17221 gcaggcctat gatttttgcg gcgcaccaag ccgtgggaac gggcgacggt cgaaaggcca
    17281 catgagtgtt gaacccccgc acgacggcag agatgggtca aaaccgacgc ggaaagtgct
    17341 ctgctccggg gttgggttgg tcgcgctcgg atttggccgc tgggtcagcc ctcaactcgt
    17401 cgagcgggca cgatccgcca ttcagctcca cgctaccgct accgccccgc cgccccatca
    17461 tcccgtcgcc ggccttgcgc catagacctt cacagcgcgt gtctcgaagg cctcggcgaa
    17521 tttctggaag gcgcggtcga acatcgcgcc catgagcagc tggaggacaa tcgacttgaa
    17581 ctcgtagtcg atgaagaagt cgaccgtcga gccgcccggc cgctcgacga aacgccactg
    17641 gttgacgagg cggcggaagg ggccatcgag atattcgacc aggatctgcg gagttgccgc
    17701 gccgcgctcg agcgtcacgc ggctcgtgaa cgtctcccgg atggcgccat agccgcaggt
    17761 catgtcggct atcaaattgg gcttctcgac cgagccgccg cggctcttca ccttcagcgc
    17821 ctcgcagagc ggcaagaact cgggatagcg ttcgacgtcg gccacgagat cgaacatctc
    17881 gcgagcggag tggggcacgt ggcgcttagt cgagtaggtc ggcatgcgtc tctctacatc
    17941 agctcaggcc gagagggagg aggggaggcc cgccacgaag cgtcgcacct cggcgcggga
    18001 gccgagcacg atcgtgcgcc gctcgccgag cgaccgctct cgctcgatca gcgcgagcgt
    18061 cccaggccgg ctgcgcgtcg ggtaattcca gatccaatcg gtgaagaacg gaatgtcgat
    18121 gcgctccggg cagcccggcg ccatgtcagg gcgcgttcgg ccgaagctcg cgagcgtgcg
    18181 ccagatggca ctcgagagac agacgcgtcg cgaaaggtcc agccagatga tcgcatctgc
    18241 acgcggaagg cggatgtcca aggtggccga gtagttgccg tccatgaccc acgcgtcgcg
    18301 ctggacaagc tcggcgacct tcacccgcca gacgggcttc tcgggctcta cccagccggg
    18361 cagccagaac tccttgtcga ggtggatcac tgggagggtg agcttgcggc cgatctcgtc
    18421 ggcgagcgtc gatttgccgg ctcccgacga gccgagaatg aggatgcgcc ggcattcggt
    18481 cgactgaagc ggggacaccg cctcaaccgc gcccaaggcg agccgccttc agcttcgcaa
    18541 agtcggcgtc ggcgtgatag ctcgagcgcg tgagcgggct cgccgagacg aggagaaacc
    18601 ctttcgcgcg cgccgtcctc tcgtaaccgg cgaaggcctc cggcgtcacg aactccttca
    18661 cctcagcgtg cttgcgcgtc ggctggagat actggccgat ggtcaggaaa tcgacgtccg
    18721 ccgagcgcag atcgtccatc acctgcaaga cctcttcgcg ccgctcgccg agcccgacca
    18781 tgatccccga tttcgtgaac atggcaggat cgagctcctt cacgcgctgg aggaggcgga
    18841 gggaatggaa gtatcgcgca cccgggcgga tcgccnnnnn nnnnnnnnnn nnnnnnnnnn
    18901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nntcctcagg aaatccggcg tcagcacctc gatcgtgacc ctggggctcc gcgcacggat
    19021 cgccgtgatg accgaagcga agtgggcagc cccgccgtcg gcgagatcgt cgcgatcgac
    19081 cgaggtgatg acgacgtggc cgaggccgag cttctcgacc gcggtcgcga cgttctccgg
    19141 ctcggcggga tcgagcggct tcggcagccc ggtcgcgaca ttgcagaacg agcaggcgcg
    19201 cgtgcagatg ctgcccatga tcatcatggt ggcgtggcgt ttcgtccagc attcgccgat
    19261 gttcgggcag cctgcctcct cgcagaccgt gtggaggcca tgctcgcgga cgatgcgctt
    19321 cgtctcgctg tagccgggtg acgtcggcgc cttgacgcgg atccaatcgg gtttgcgcaa
    19381 gagcgccgta tcgggccgcg cctgcttctc cggatggcgc gggcgctccg gctcggactt
    19441 ccgcgtcgtg tcgaccaccg tcaccatggg ctcgtccttc cgatcaccag cgagaggtgg
    19501 cgaaggattg gccggccgtc aaggctctgg aggctaagcc cggccttcat ggtataggag
    19561 gccaacaccc aggaaagggc cgctcatgac agaccatctg aaggacgtgc tgaagacgct
    19621 tgatgccaac gaggagaagg ccgtcgagcg gctgttcgag cttctgcgca tcgacagcat
    19681 ctcgaccgat cccgcctaca agggcaagtg ccagaaggct gctgagtggt gccgcgacca
    19741 gctcgccgag atcggtttcg cgtcggccaa ggtggtgccg acgacgggcc accccatggt
    19801 cgtcggccat gaccgcaggt cggtgccgaa aggcatgccg cacgttctct tctacggtca
    19861 ctacgacgtg caaccggccg acccgatcga tttatggaca tcgccgccct tcgagccgcg
    19921 catcgccacc gagaagggga acggcaaggt gatcgttgcc cggggtgccg aggacaacaa
    19981 gggccagctt atgacgttct tcgaggcggc gcgcgcatgg aaaaaggtcg cgggcgagct
    20041 gcccgtcgcc gtcacggtgc tgctcgaagg cgaggaggag tgcggctcgc cctcgctccc
    20101 tggcttcctc aagacgcacg ccaaggaggt gtcctgcgat ctggccctcg tctgcgatac
    20161 gggccaatgg gacaagggga caccggcgat caccaccatg ctgcgcggca tggcggcgac
    20221 agagatcact gtgaagtgcc cgagccgcga cctgcattcc ggcatgtacg gcggcccggc
    20281 gatgaacccg atacgtgcac tcgcccgcat cgtcgcctcg ctgcacgacg acaaggggcg
    20341 cgtggccgtt ccaggcttct acgatggcat cgtcaagccg acgaggaagc agctcgcgca
    20401 atgggagagc ctcggcttcg acgacaaggc gttcctcgga gcagtcgggc tcaagacgcc
    20461 ggcgggcgag cagaagtgct ccgtcctcga gcagctctgg gcacggccga cctgcgagtt
    20521 caacggcatc accggcggct accagggcgt cggcaccaag acggtgatcc cgtcggaggc
    20581 ctcggtgaag atcacctgcc gcctcgtccc ggggcaggac ccgaagaagg tcttggcggc
    20641 gctgaaggcg cacgtcctcg cggccgcgcc cgccgacagc gaggtgaact ttgcctatct
    20701 gaggggtagc cctgcgatcg ctttcgacac tgaggcttcg catatccgcg ccgcggcgga
    20761 ggggctcgcc gaggaatggg gcaagcccgc cgttatgatg ggctgcggcg gctccatccc
    20821 gatcgtcggc agcttcaagg acgtgctagg catggacagc ctgctcgtcg gtttcggcct
    20881 cgaggacgac cgcatccact cgccgaatga gaagtacaac ctgtcgagct atcgcaaggg
    20941 cgcgcgcagc tgggcgcgca tcctggcgaa gctcgcgaga tgaggcctgc gcgaacgccc
    21001 gagtgaaagg cttgacccgt ccgccagcac tcccgtacag aggcgcggcg atgggcccgt
    21061 agctcagcgg tagagcaagc gccttttaag cgagaggtcg ttggttcgat cccaaccggg
    21121 ctcaccaata aacgccgccg ggccatgggt ccggcggcgt ttcaattggc gcgcgatcgt
    21181 gctcactact cggccgcggc cgatacgcgc gcagacgttt cggttgtcgg cgagagatag
    21241 gcaccctgac gcaagagctc ctgctggatg aggcgcggcg cgacatgtcg cggcgcttgg
    21301 ctgcgcgcga ccgccagcgc tgcggcgatg cccgccgcct gtcccgtcat ccagcactgc
    21361 gggatctcgc gcaggaacga gtgcgaggtg gcatcgcagg cgacatgccg gccggcagca
    21421 atcacgccgt cgagaattgc cggcaccagc gcaccgtagg gcaccgagat gttcgggaac
    21481 ttcggcgaca aggacgtcga gacgccgatc tcgtccggcc agacctcgcc tgtatcccat
    21541 tgtgcacgca cgaccttgcg gacgccgccg atgcgccggc tgtggcggac gccgatctgc
    21601 ggcccgccaa gcatcaggaa ggcccgctcg aagccgggca tgttgcggcg atagaaggtg
    21661 atgtggtggt cggtgagctc gcgtgagcgg atctcgacct cggtcaggtc ctcgacgttg
    21721 acggcactat agcccgtgag ccgcggcccc atgaacaggg caacgtcgcg gcgccagccg
    21781 acgaacggct tctcgaatga gcggagctcc tcgcgaccct gctggagcag gtcggagtag
    21841 cgcgccttct cggtcgtgcg gaaggcgatg aaccggtcca tgtcgacgcc gcccatcagg
    21901 aaggcggtgt tgatgcagtg gtggatgtcg cccgtgtcga tgtcggattc caagggggcg
    21961 ccggcgcggg cgagcagatc ggcgtcgccg gtagtatcga cgacgacctt ggcgaggacg
    22021 gcctgccggc cttccttcgt ctcgaagatg gcgccgcgga gcgcgccatc ctcgacgatc
    22081 ggcatggatg cccaggagtg cagcaggagg cgtgccttct cctcgaggac catccgcagg
    22141 gagagtgatt tgagcgcttc cggatcgatc gtcggcgacc aggtgacgat gccgtgatat
    22201 gcagcggtgc gctgcgccca gtaggcggca gttgcggcat cgcgcgagcc ccagtcggcg
    22261 cggggcgggc cctggatcgc gtccttgggc agtcggtcca tgagctcggc ggcgataccg
    22321 gcgatcacct gacggccggt ccagtcggac atgcggtcga tccagatcac gagaccgccg
    22381 gtcgagaggc cgcc
//
LOCUS       DAORNH010000034        22261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1804_length_22261_cov_2.664550, whole genome shotgun sequence.
ACCESSION   DAORNH010000034 DAORNH010000000
VERSION     DAORNH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22261
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1804_length_22261_cov_2.664550"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /locus_tag="PK264_05665"
     CDS             complement(<1..1412)
                     /locus_tag="PK264_05665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD75413.1"
                     /translation="MTDFQSLGLAAPLLKALVDEGYTTPTPIQVQALPPVMAGKDVLG
                     IAQTGTGKTAAFALPILHRLDANRRQPLRKGCRVLVLSPTRELASQIADSFRTYGRHA
                     RLTVAVIFGGVGHAPQTRAMLQGVDVLVATPGRLLDHMSMRNVTLEGTEILVLDEADQ
                     MLDLGFVKPIRQIVSRLPSRRQSLFFSATMPQDIASLAAELLTEPQRIAVAPVATTAE
                     RVSQRVIHVEHSAKRALLAELLADEAMSRTLVFTRTKRGADRVARHLESCNISAAAIH
                     GNKSQRQREIALEAFKRSDVRVLVATDIAARGIDIDQVTHVINYELPEVPEAYVHRIG
                     RTARAGAEGSAISLCDREERDLLRAIERLTRQSIPSETRTVSAAPSTAATTDAGEPHE
                     AVERPRPTERHAERRRDHRGPARHRHRDERAERPRSGDGDRQEHDRHADQPDDRECRS
                     AVQGLWQRRARLSPRPCRLC"
     gene            complement(1747..2679)
                     /locus_tag="PK264_05670"
     CDS             complement(1747..2679)
                     /locus_tag="PK264_05670"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRD75414.1"
                     /translation="MDVLLQGAVHRISVGSKSFAAAARLLPPDKRASVRLLYTWCRHC
                     DDAIDGETLGFRAAPDHIPPGEHLARLVAGTDLALNGRSTEPEFRALERVVAEHAIPA
                     RYPRELLAGMEMDVRGDRFQSIEDTLSYCYHVAGVVGVMIAIILDARDGATLERASDL
                     GIAFQLTNIARDIVADAERGRVYLPSTWLAVEGLTTETILDQERRGGLFKVAQRLLSL
                     SDGYYESAVAGIARLPFRSAFAIATARRVYRRIGTRILEQGATSWDQRIATSRREKAV
                     ACVLAMADVVALNAIPDGSARPRDGLWTPQGLIA"
     gene            complement(2716..3153)
                     /locus_tag="PK264_05675"
     CDS             complement(2716..3153)
                     /locus_tag="PK264_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRD75415.1"
                     /translation="MKRLVPVLASIACLALSALPALSQEGNAEEGEAVFRKCATCHKV
                     GPDARNFTGPVLNGVIGRPAGTVPEFAYSDLNKAAGANGLVWTEQLIFEYLEDPAEFL
                     KKFLTSKGKQDLAEGTTRMVFKLTDETDRRDVIAYLKRFSPAK"
     gene            complement(3258..4183)
                     /gene="pufM"
                     /locus_tag="PK264_05680"
                     /pseudo
     CDS             complement(3258..4183)
                     /gene="pufM"
                     /locus_tag="PK264_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit M"
     gene            complement(4236..5060)
                     /gene="pufL"
                     /locus_tag="PK264_05685"
     CDS             complement(4236..5060)
                     /gene="pufL"
                     /locus_tag="PK264_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009521 - photosystem [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center subunit L"
                     /protein_id="HRD75416.1"
                     /translation="MSMLSFERKYRVRGGTLIGGDLFDFWVGPFYVGFFGVTTVFFVF
                     VGVGLILWGAALGPTWNIWKISINPPPAAYGLGLAPLQEGGIWQLVTICAIGAFVSWA
                     LREVEICRKLGIGFHVPFAFSFAILAYVTLVVVRPVLMGSWSYGFPYGIISHLDWVSA
                     TGYQYGNFHYNPAHMIAITFFFTTCLALALHGGLILSATNPAAGEKVKSPEHENTFFR
                     DAIGYSIGTLGIHRLLALSAGFWSAVCIVISGPFWPENKPWVDWWNWWLTLPIWGR"
     gene            complement(5198..5410)
                     /gene="pufA"
                     /locus_tag="PK264_05690"
     CDS             complement(5198..5410)
                     /gene="pufA"
                     /locus_tag="PK264_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, alpha subunit"
                     /protein_id="HRD75417.1"
                     /translation="MWKVWLLFDPRRVLVALFTFLFSLAVLIHFILLSTDRFNWLEGP
                     RATRPKASIEHIMPTDPIAPVRTTIG"
     gene            complement(5422..5658)
                     /gene="pufB"
                     /locus_tag="PK264_05695"
     CDS             complement(5422..5658)
                     /gene="pufB"
                     /locus_tag="PK264_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030096 - plasma membrane-derived
                     thylakoid photosystem II [Evidence IEA];
                     GO_process: GO:0015979 - photosynthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="light-harvesting antenna LH1, beta subunit"
                     /protein_id="HRD75418.1"
                     /translation="MADRPTGSMSGLTEQEAKEFHGLFMFGFTVFTAVAVVAHILVWM
                     WRPWLPTVRGYTSLSDGSVQTLAALSQQLQQFIG"
     gene            complement(5796..6092)
                     /locus_tag="PK264_05700"
     CDS             complement(5796..6092)
                     /locus_tag="PK264_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75419.1"
                     /translation="MQLNWQLPASAHPAPGSAILAADDAPSRAAGGHKVRRRTGFQYW
                     LIFSVCLFVLLWAGLVERLNPLFWVARRPDGNVQPLWAASKETAHRCTSLAFGG"
     gene            complement(6105..7538)
                     /gene="bchZ"
                     /locus_tag="PK264_05705"
     CDS             complement(6105..7538)
                     /gene="bchZ"
                     /locus_tag="PK264_05705"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Z"
                     /protein_id="HRD75420.1"
                     /translation="MLISDHDRAGGYWGAIYVFTAIKGLQVVIDGPVGCENLPVTSVL
                     HYTDALPPHELPIVVTGLGEDELGQHGTEGAMRRAHRSLDPDLPSVVVTGSIAEMIGG
                     GVTPEGTNIQRFLPRTIDEDQWQCANRAMSWLWSEYGLKRVPAKKPRKAGEKPRVNII
                     GPSYGTFNMPSDLAEVRRLIEGIGAEVNLVFPLGSHLADVPKLANADVNVCLYREFGR
                     MLCEALDRPYLQAPIGLHSTTAFLRSLGEHLGLDPEPFIAREKHTTIKPLWDLWRSVT
                     QDFFCTASYGIVAGETYARGVRNFLTEELGLPCTLAVSRRPGVKPDNELVRKALKETP
                     PLVLFGSYNERMYLAEAGGRSIYIPASFPGAVIRRHTGTPFMGYAGAVYIVQEVCNAM
                     FDALFNILPLGTKLDEIEPTPARLAATISWDEDAQVELERHLDREPVLIRISAAKQLR
                     ERIERHARSTSGSHVSLDHVRQNIGST"
     gene            complement(7538..>8550)
                     /gene="bchY"
                     /locus_tag="PK264_05710"
     CDS             complement(7538..>8550)
                     /gene="bchY"
                     /locus_tag="PK264_05710"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chlorophyllide a reductase subunit Y"
                     /protein_id="HRD75421.1"
                     /translation="SALKTLAEAKDVLAGAMLAYARNEVSQGPVQAPRGGRAERPGVA
                     LLGEMFPADPIVIGRLLEPLGLAAGPVVPTREWRELYAALDCGVAAAIHPFYTASIRE
                     FETAGRKIVGSAPVGHAGTAAWIAAIGDAYGVPRAVVGRASNAILPAIRGVLAAHPIK
                     GRITLSGYEGSELLVARLLVESGADVRYVGTACPRTPYSEPDRAWLESKGVPVTYRAS
                     LEQDIAAVDAFDPDLAIGTTPVVQHAKQRAIPALYFTNLISARPLMGPAGAGSLAQVV
                     NTAIANRARFDAMTSFFTGVGQGDAAGIWTDVPADRRDFKETYRRKLAKQAAARKSEE
                     MV"
     assembly_gap    8551..8560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8561..9140)
                     /locus_tag="PK264_05715"
     CDS             complement(<8561..9140)
                     /locus_tag="PK264_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HRD75422.1"
                     /translation="MTNGDVSTGRGSEVVAPESGETPSAEQPGAARTANLSCHAGKDE
                     LRRAAEASGKSEMLDRYAADYPVGPHDKPQSMCPAFGSLRVGLRMRRTATVLSGSACC
                     VYGLTFTSHFYGARRTVGYVPFDSETLVTGKLFEDIREAVFKLADPASNDAVVVINLC
                     VPTASGVPLQLLPKQINGVRIVCIDVPGFGVPT"
     gene            complement(9172..10191)
                     /locus_tag="PK264_05720"
     CDS             complement(9172..10191)
                     /locus_tag="PK264_05720"
                     /EC_number="1.3.7.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllide a reductase iron protein subunit
                     X"
                     /protein_id="HRD75423.1"
                     /translation="MTASCGGAVAMLDEQVRVDRLRREAATAPAPVVIAPPTKATQII
                     AIYGKGGIGKSFTLANLSYMMAQQGKRVLLIGCDPKSDTTSLLFGGRACPTILETSAK
                     KKLAGDQVAIGDVCFKRDGVFAMELGGPEVGRGCGGRGIIHGFELLEKLGFHDWGFDY
                     VLLDFLGDVVCGGFGLPIARDMCQKVIVVGSNDLQSLYVANNVCSAVEYFRKLGGNVG
                     VAGLVVNKDDGTGEAQAFAEAAGIPILAAIPANEDIRRKSASYEIIGRPGGPWSQVFE
                     GLATNVADAPPLRPKPLSPDALLGLFKSEAVGRGVVLEPASIADMRGAAANEKKSLEV
                     IYDSV"
     gene            complement(10188..11126)
                     /gene="bchC"
                     /locus_tag="PK264_05725"
     CDS             complement(10188..11126)
                     /gene="bchC"
                     /locus_tag="PK264_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyll synthesis pathway protein BchC"
                     /protein_id="HRD75424.1"
                     /translation="MQTAAVIMKGPGALSVDRAELRTPGDGDLLIDVEWSGISAGTER
                     LFWSGRMPQFPGMGYPLVPGYETVGRVASAPDSAGIAVGARVFVPGARAFTDVRNLFG
                     GSAQRLVVESSRVVQVPEPLAEQSALLALAATAHHAAPLGQPAPELIVGHGALGRLLA
                     RLAMLAGGPPPVVWERHAGRREGASGYSVIDPSEDGCRSYNRICDVSGSTGVLDELVG
                     RLAPGGEIVLAGFYEEPLSFAFPPAFMRELRLRVAAQWQAADLAAVAGLVANGRLSLD
                     GIITHVTAARDAATAYRQAFEDPGCLKMVLDWRAFP"
     gene            complement(11246..12310)
                     /locus_tag="PK264_05730"
     CDS             complement(11246..12310)
                     /locus_tag="PK264_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD75425.1"
                     /translation="MSTRFQRWAARMPLVRGKAAREAQAAFDLCAGFVYSQTLFASVK
                     LGVLKAVADGPIGLEALVARTGLPQQSARTLINAAIALGLLSRRSHGRIGLGMRGAAI
                     SANPGIAAMVEHHAMLYADLADPVALLARPKADTRLAGYWPYAASAAPHGIKPDCAKP
                     YSGLMTTSQSLLADNIIDAYPFHRHRRVLDVGGGEGAFLATLSARHPGLALHLFDLPA
                     VVELAESRLASLGLGGRISTTGGDFARDPLPTGFDAATLIRVAHDHDDDRAVEIFANI
                     RRALVPGGALVIAEPMSGIRGAERVSDVYFAFYLLAMGSGRPRSPGQLEELLRAAGFA
                     SFRKAATAQPMIASVLVASA"
     gene            12561..>13575
                     /locus_tag="PK264_05735"
     CDS             12561..>13575
                     /locus_tag="PK264_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD75426.1"
                     /translation="MSRDRVVVVGAGVGGLVAAMMLAARGVETVLVEKQTTVGGRIRE
                     LTVAGRSIDAGPTVLTLRHVFDGIMTACGLDLAGLVRLTRADVLARHAWSPEERLDLF
                     ADRLRSADAIGSFAGAAARRGYLDFCAHSRRIYETMDPIFMRSERPTPIGMVKAVGLS
                     GLGGLISSAPFTTLWDLLGRYFPDPRLRQLFGRYATYCGSSPFLAPATLALIAHVEQD
                     GVWLVEGGMRAFADALAVAARTKGVEIRTGTSVAEILIKDGRARGLRLATGETIETAA
                     VVSAVDVGALADGLLGADARSAVDGGASTPRSLSAATWGLVARVSGLTLAHHTVLFSR
                     SSPQ"
     assembly_gap    13576..13585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13586..>13960
                     /locus_tag="PK264_05740"
     CDS             <13586..>13960
                     /locus_tag="PK264_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CrtD protein"
                     /protein_id="HRD75427.1"
                     /translation="GVPPSDPTVYICAQDRSDSGERRADAERIFIITNAPADGGRSLG
                     TPEEREACETRMMGTLARCGLTLMIDGMVHTGPAEFGALFPGTQGAIYGMASHGWAAS
                     FRRPGTRSRIHGLYLAGGSVHPG"
     assembly_gap    13961..13976
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13977..14940
                     /locus_tag="PK264_05745"
     CDS             <13977..14940
                     /locus_tag="PK264_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HRD75428.1"
                     /translation="TFGASGCAEPDRGSDLDTPVASGGYAWWYVDALSDDGRFGLSLI
                     AFVGSVFSPYYAHGRRRGRADPENHVAINIALYGEGGYRWAMTERGRRSLSRTPNLLQ
                     IGPSRLLRSGSAWHAQLDEICVPVPRRIRGSFTVEPLAYGRQTYMLDDCGRHMWRPLA
                     PRARMTVMLEDPRLTWSGPAYVDHNRGTEPLEAAFSRWSWSRSVETDRTVIFYDAKMR
                     SGASKEHHIAVGADGAPTPLAAPPMASLGQSLWRLPVEVRTDAGSTPERIATWEDGPF
                     YARTLVRHLVGGTPVTSIHEALSLDRFKRRTVQMLLPFRMPRRS"
     assembly_gap    15023..15151
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15286..15777)
                     /locus_tag="PK264_05750"
     CDS             complement(15286..15777)
                     /locus_tag="PK264_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRD75429.1"
                     /translation="MHDRGDRALWKPLLASAAGWSGVAVLGGIATTIGPWYWALKKPW
                     FQPPDWAFGPAWTLIFALTAIASAMAWRAGPDNAGRRRWVSALVLNGLLNVAWSVLFF
                     YLRRPDLALIEVGLLWLSIAVLVWMARSRSSPAALLLVPYLVWVSFAAVLNAAVVRLN
                     APF"
     gene            complement(15770..16990)
                     /locus_tag="PK264_05755"
     CDS             complement(15770..16990)
                     /locus_tag="PK264_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl diphosphate reductase"
                     /protein_id="HRD75430.1"
                     /translation="MTIDTAFDVVVVGGGPSGATAAHDLASAGCRVALLDRMGRIKPC
                     GGAIPPKLIEEFEIPEQLLVARITSARMVAPSEAHVDIPIENGFVGMVDREVFDDWLR
                     QRAASAGAVLLGGIFEDLVREADGDVTVLYRAAGDDAGGSLRRLRARAVIGADGAISR
                     VARVAMPDVERARCVFAYHEIIESPENTENHVKEPARGFDSARCDVIYRGTTSPDFYC
                     WIFPHGRTTSVGTGSASKGFALREAVADVRRHYGLAGQRTVRREGAPIPMRPLRRWDD
                     GRNVVLAGDAAGVVAPASGEGIYYAMLGGRFAAEAAAQFVTTGDARALRSARRQFMRL
                     HGRVFWVLGVMQYFWYRSDRRRERFVTICRDPDVQRLTFEAYMRKRLVRAKPFAHVRI
                     FFKDLAHLLGLVRA"
     gene            complement(16987..18375)
                     /locus_tag="PK264_05760"
     CDS             complement(16987..18375)
                     /locus_tag="PK264_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HRD75431.1"
                     /translation="MTERAVVGLGWLGIVRLGLVQSALGAIVVLTTSTLNRLMVIELA
                     LPAMIPGALVALHYAVQILRPRMGYGSDVGGRRTPWIVGGMMVLALGAVLAAAATSVM
                     SVSFVPGLVLAVIAFLVIGLGVSAAGTNLLVLLAKQTAKDRRAAAATIVWLMMIAGFA
                     VTAIVVGRLLEPFSFGRLIVVTSGVGVAAVLVAIAALAGIEPRALATTTGDAEAKPAM
                     PEFRAALAEVWVDSQARRLTIFIFVSMLAYSTQDLILEPFAGLLFGYTPGQSTMLSGV
                     HHGGVFAGMVLVAVCATAIGGRWLGSLRLWTIGGCLASAAALIAVIAAGFAGPAGPLK
                     AAVVVLGIANGAFAIAAIGTMMRMAGEGRAGREGTRMGLWGAAQGIAFGLGGFGGTVM
                     IDVARGLVSSPSHAYALVFGIEAALFVTSAVLAFGLTEYGARGRPRDWIWTSGTRAGP
                     TVADVPRAGAAS"
     gene            complement(18389..>18671)
                     /locus_tag="PK264_05765"
     CDS             complement(18389..>18671)
                     /locus_tag="PK264_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriochlorophyll/chlorophyll a synthase"
                     /protein_id="HRD75432.1"
                     /translation="GSLPVMLGPEFAARTASVVMIVPQLVVAALLMAWGRAGHGIAIV
                     VICLLQVILMRRLLSDPRRYAPWYNSTGIGLLVLGMMIAAAAIRSLPAS"
     assembly_gap    18672..18759
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18760..19291)
                     /locus_tag="PK264_05770"
     CDS             complement(<18760..19291)
                     /locus_tag="PK264_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRD75433.1"
                     /translation="MIVLTNEKVPSIAAVIELLKPVTWFPPMWAFACGVVSSGMPASG
                     SWSRILLGIVLVGPLVCAASQAINDWHDRHVDAINEPKRPIPSGRIPGRWGLYIAAGW
                     CLLSLGAASVLGRVVLGATVLGLVMAWAYSAPPLRLKRNGWWGNAACGLCYEGLAWVT
                     GAALMLDQMPSTSILAM"
     gene            complement(19381..19548)
                     /locus_tag="PK264_05775"
     CDS             complement(19381..19548)
                     /locus_tag="PK264_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75434.1"
                     /translation="MTFESMAGLIEFVFVGALSIGVGIWQVIKMRRELKRDREAAATA
                     VADPGTSRERE"
     gene            complement(19611..20915)
                     /locus_tag="PK264_05780"
     CDS             complement(19611..20915)
                     /locus_tag="PK264_05780"
                     /inference="COORDINATES: protein motif:HMM:NF023330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HRD75435.1"
                     /translation="MSPLPQEFPLRRALNEEAHARPPEALRAPQRITFLALYDAGIER
                     VKHEAPLRELCKRYMIEPPPAGVSHFSGDCGKFRVKWEKHTEFTRYKFIIGGTSPSPF
                     AEPALQSVPQEWLSSLPGEIISATHVALLPFGRETPDVEKLAGEHFIGNTIVGSLVAD
                     GGALAITDYRIRDDGFSRLLVLDRNLTPRQAGRTVQRLLEMEAYRILAMLALPIAREL
                     SPSLTTAERDLVDITAAMADVAAVDEPALLDRLTRLEAQIQKRHFETASRFSAAEAYY
                     RLIQRRTEELREKRVEGLQTFREFVERRLSPAMSTITATSARHEKLALRIAQATQLLS
                     TRVDVSRQQHNQRVLESMNQRVLLQLRLQQTVEGLSVAAITYYLVGLLSYLFKGAAVA
                     GLVGRADLLIAISVPLVAALVAIGVRGARRFLGGGSDHSKSA"
     gene            complement(20912..21916)
                     /locus_tag="PK264_05785"
     CDS             complement(20912..21916)
                     /locus_tag="PK264_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD75436.1"
                     /translation="MMRAEAHSQDGDVMAITTDARVDDFVNSTRFSLDGVIWPNATMS
                     RLVDIEGRVVHIQQMGRGPPALLIHGTGASTHSWEGLVDELAADLHLTLVDLPGHGFS
                     APAASSDMSLEGYARSLARLVSVLGIAPQLVIAHSAGAAVAVRMALDGSLARSCRIIA
                     INGAFLPFGGLGRWLFPPMARLLAAGPFTARLFARHAERAGAVERLLIGTGGKPPQRS
                     IGFYRLLLKQPAHVQSALDMMANWSLERLSAELPRLRAPLDLVACGDDRAVPAETAWT
                     VKSLVAGASVHYLRDAGHLAHEEQPAAVAAIVRGAIAGLASCGREEPLRSTTGRHSDL
                     "
     gene            complement(21913..22176)
                     /locus_tag="PK264_05790"
     CDS             complement(21913..22176)
                     /locus_tag="PK264_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75437.1"
                     /translation="MIVILTDGRANMSRRGAPGRKLALLDAESAASQLRAEGHASLII
                     DTSPRGNADAAGLARLMGASYNPLPHAEARAIAEEIQKKSAHA"
BASE COUNT         3984 a         7272 c         7210 g         3542 t
ORIGIN      
        1 atgcataatc ggcatggccg gggcgatagg cgagcgcgac gttgccataa tccttggacc
       61 gctgatcgac attctcgatc atcaggctga tcggcgtgcc ggtcgtgttc ctgccgatcg
      121 ccgtcgccgc tgcgaggccg ttccgcacgc tcgtcgcgat gtcggtggcg tgccggaccg
      181 cgatgatccc ggcgccgctc cgcgtgtcgc tccgtgggcc tcggtcgctc gaccgcctcg
      241 tggggctcgc cggcatcggt tgtggccgcc gtcgacgggg ccgctgatac cgtgcgcgtc
      301 tcagagggta tcgactgccg cgtcagccgc tcgatcgcac ggagcagatc gcgctcctcg
      361 cggtcgcaga gcgagatcgc gctgccttct gctcctgccc gcgcggtacg gccgatgcgg
      421 tgcacgtaag cctcaggcac ttcggggagc tcgtagttga tgacgtgcgt cacctggtcg
      481 atgtcgatgc cgcgcgccgc gatatcggtc gcgacaagca cgcgcacgtc cgagcgcttg
      541 aaggcctcga gcgcgatctc gcgctggcgc tggctcttgt tgccgtgaat agcggccgct
      601 gagatgttgc agctctcgag gtgacgcgca acacggtcgg cgccgcgttt ggtgcgcgtg
      661 aagaccagtg tccgcgacat ggcttcatcc gcgagcagct cggcgagcag tgcgcgcttc
      721 gcagaatgtt ccacgtggat cacgcgctgc gagacgcgct cggccgtggt tgcgaccggc
      781 gccacggcaa ttcgctgcgg ctcggtcagg agctcggccg caaggctcgc gatatcctgc
      841 ggcatcgttg ccgagaagaa gaggctctgg cggcgcgatg gcagccgcga gacgatctgg
      901 cggatcggct tcacgaagcc gaggtcgagc atctggtcgg cctcgtcgag caccaggatc
      961 tcggtgccct ccagcgtcac gttgcgcatc gacatgtggt cgaggaggcg gcccggggtt
     1021 gcgacaagca cgtcgacacc ctgcagcatc gctcgcgtct gcggggcatg gccgacgccg
     1081 ccgaaaatga cggcgaccgt gagccgcgcg tggcggccgt aagtgcggaa gctgtcggcg
     1141 atctggctcg cgagctcgcg cgtcggcgag aggacgagga cacggcagcc cttgcgcaga
     1201 ggttggcggc ggttggcatc gaggcggtgc aggatcggca gcgcgaaggc cgccgtcttc
     1261 cccgtgcccg tctgggcgat gccgaggaca tccttaccgg ccatcacggg cggcagggcc
     1321 tgcacctgaa tcggcgtcgg cgtcgtatag ccttcgtcca ccagggcctt gagaagcggc
     1381 gcggcgaggc cgagggactg gaaatctgtc acgtggagac tttcatgtga atccgcactc
     1441 gctcgccggg ccgaaacttg aacgggcgcg gggcgtgcgg atcgggtggg tgaggacgcc
     1501 ccgcgtggcc tgggcgaacg atcgagagga tcggggcgct caacagggag accgggctgc
     1561 caccgcacat cgccatcgag gcgaaaccgg cagaaatggt cccttcacgc ggctggagca
     1621 ccgagaatcg caatgcttgc gatgcgcgct tctctcatgt tgcattgcga aagtcaaacg
     1681 cttccagcgt tgcacaggtc agcctggatg gcctggtggg ccgtggccgc agcgacgcct
     1741 tccgcatcag gcaattagac cttgaggcgt ccaaagaccg tcgcgaggcc gggcggagcc
     1801 gtctggaatt gcatttagcg cgacaacgtc ggccatggcg aggacgcagg caacggcctt
     1861 ttcgcgccgg ctcgtcgcga tgcgctgatc ccagctcgta gcgccctgct ccagaatccg
     1921 cgtgccgatc cgccgataga cccgtcgagc ggtggctatg gcgaaggccg aacggaacgg
     1981 cagcctggca ataccggcga ccgccgactc ataatatccg tccgaaagtg aaagcagccg
     2041 ctgtgccacc ttgaagagcc cgccgcggcg ttcctggtcc agtatcgtct cggtcgtgag
     2101 gccttcgacg gcaagccaag tggacgggag gtagacccgc cctcgctccg cgtccgccac
     2161 aatgtcgcgg gcaatgttcg tcagctggaa ggcaatgccg aggtcggacg cgcgctccag
     2221 cgtcgcgcca tcgcgggcat ccaggatgat agcgatcatc acgccgacga cgcccgcaac
     2281 gtgatagcag tagctgagcg tatcctcgat cgactgaaac cggtcgccgc ggacatccat
     2341 ctccatcccg gcaagcagtt ctctcggata ccgggccggg atggcatgct ccgccacgac
     2401 acgctcgagc gcccggaact cgggctccgt cgagcgaccg ttgagggcga gatcggtgcc
     2461 cgcaacaagg cgggcgaggt gctcgccggg aggaatgtga tccggggctg cccggaaccc
     2521 gagcgtctca ccgtcgatcg catcatcgca atggcggcac catgtgtaga gcagccggac
     2581 gctggccctt ttgtccggtg ggagcagccg agcagcggct gcaaagctct tggagcccac
     2641 actgatacgg tgcaccgcac cctgcaacag cacgtccatg cggtctatcc acgaaccagc
     2701 gtcattccgt ttcgctcatt tggccggcga aaatctcttg agatacgcta tgacatcgcg
     2761 ccggtcggtc tcgtcggtca gcttgaagac catccgggtg gtcccctcgg ccaggtcctg
     2821 tttgcctttc gaggtcagaa acttcttcag aaactctgcc gggtcttcga ggtattcgaa
     2881 gatcagctgc tcggtccaga cgagcccatt ggcaccggca gccttgttca ggtcggagta
     2941 ggcaaactcg ggaacggtcc ccgccggcct gccgatcact ccgttcagca cgggaccggt
     3001 gaagttgcgg gcatccggtc cgactttgtg gcaggtcgcg cattttcgga aaaccgcctc
     3061 tccttcctcg gcgttgccct cctgagacag ggcaggaagt gcgcttaggg caagacaggc
     3121 gatcgaggcg aggacgggaa caagacgctt cataaagact gctccattgg tctagcggtc
     3181 ggcggctggt cgcgggcata cggccctccg gcgagtgccg gcgttcggga aaccgtgagg
     3241 atcgatttcc cgctctgtca cttcggtgag taggcagcgg gcgcaacact gtgcttcacg
     3301 gcccagtcgt accaattgtc gacagccgtc ccggtgagca ggatgccgat gccgcctgtc
     3361 agaccgcgaa ccaccaggcc cagcgatgta tcgactccat cgtggcattg aagcccatcg
     3421 tccagcgcca aaagagtgcc gcccgttccg acgcagtccc gcgatcggtg atctgctcga
     3481 tctccctctc gccgccataa cggctcacgg cgagaattgt cgcggcatgc atcgcgaaga
     3541 gcagtgcaga accgtaaagg aaggcgatcg agagcatatg gaatggattg tagaagaggt
     3601 tcccataatt gagcgagaac gagttggtcc agtcgaggtg cgggaaaatg ccgaatggca
     3661 cagcctcgct gaagttgccc atcaggaccg gccggatgaa gccgagaacg aggaacagcc
     3721 atatggccgc cgcgaatgcc caagggacgt gcgttccaag accgagcgcc cgagctcggc
     3781 gatacatgcg cacccaccag agcaggatcg atgcggtcag aaaaaacccc gcgatcagcc
     3841 accagccgcc ctgattgagg ggcgggaagg tcaggccata tcgccgcggc ggcggatcga
     3901 gcgacaaata gaagagccgg cggacgaatt ggacaggatc ccaattcacc gatgcccaca
     3961 tgttgaggcc aatgatctcg atcgcgatga tcccgcagac gatcgacaga aatcccgttg
     4021 tgccaaggta gatcggaccg atctgggcgt cgccgatgcg ccccgcccaa taggacatga
     4081 agggcttgcc agagcgctga ctgtcgccct tgggcaacgc gatacccatg tcgatcggtc
     4141 cgtggacttg aacgcgggtg aagatgttct ggtactcggc catggcctgt catccggctt
     4201 gagggaatgc gttgcggctt tcgaggtgcg cgagatcagc gcccccagat cggcaatgtg
     4261 agccaccagt tccaccagtc gacccacggc ttgttctcag gccagaatgg cccgctgatc
     4321 acgatgcaga cggcgctcca gaatccggcg ctcagagcaa ggagccggtg gatgccgagg
     4381 gtgccgatcg agtagccgat cgcgtcacgg aaaaaggtgt tctcgtgctc gggggatttc
     4441 actttctcgc ccgcggccgg attggtcgcc gacaggatca ggccaccgtg gagtgcaagt
     4501 gcgagacatg tcgtaaagaa gaacgtgatg gcgatcatgt gcgccggatt gtaatggaaa
     4561 ttcccgtact gataacccgt tgccgacacc cagtcgagat gactgatgat gccgtagggg
     4621 aatccgtagg accacgaccc catcagaacc ggacggacga cgaccagcgt cacgtaggcg
     4681 agaatcgcga agctgaatgc gaacggcaca tgaaatccaa ttccgagctt gcggcagatc
     4741 tcaacttctc gcagcgccca tgagacgaag gccccgatcg cacagatcgt cacgagctgc
     4801 cagatgccgc cctcctgcaa cggtgcgagc ccgagaccgt aggccgccgg aggcggattt
     4861 atgctgatct tccagatgtt ccaggtaggc ccgagggcgg ctccccagag aatgaggccg
     4921 acgccgacga agacgaagaa cactgtcgtc acgccgaaga agccgacata gaaggggccg
     4981 acccagaagt cgaacagatc gccgccaatc agcgtgccac cccttacccg gtatttccgc
     5041 tcgaagctca gcatcgacat ttgaggctcc cggaccgtac cgccgcgatc catccagagt
     5101 tgactgcccg gcagggcttg ctgtgacagg cgagacgccc gccgccgtgg cggaaggcga
     5161 tccgaagggg ccgccttccg cgaggttcgt agtcacgtca tccaatcgtc gtgcgaactg
     5221 gcgcaatcgg atcggtcggc atgatgtgct cgatcgaggc cttcggccgc gtggcccgcg
     5281 gaccttccag ccaattgaac cgatccgtgc tcagaaggat gaagtgaatg agtacggcca
     5341 ggctgaacag gaaggtgaac agcgcgacca gaacccggcg cggatcgaaa aggagccaga
     5401 ctttccacat ggatccctcc gttagccgat gaactgctgg agctgctggc tcagggcagc
     5461 gagcgtctgc accgagccgt cactgaggct cgtgtagccg cgtacggtcg gcagccacgg
     5521 ccgccacatc cacacgagga tgtgcgcgac gacggcaaca gccgtgaaca ccgtaaagcc
     5581 gaacatgaag aggccgtgga actccttcgc ctcttgttcg gtcaatccgg acattgaacc
     5641 ggtaggtcgg tcagccatgc tgtttctcct caagttgcgg ctaactgcca gtgctgcgtt
     5701 gtccctgcgc agccaggttc gtcgtggcag gactgccgcg gcgagcttcc cggaggccgg
     5761 cgtgccagtg cggctcaacg ccggctccga tggagtcaac ctccgaatgc caggctcgtg
     5821 cagcggtgtg cagtctcctt ggaggctgcc cagagcggct gcacgtttcc gtcgggcctg
     5881 cgtgcgaccc agaacagcgg gttcaaccgc tcgacaagtc cggcccacag gaggacgaac
     5941 aggcagacgc tgaaaatcag ccagtactgg aagcctgtgc gtctgcggac cttgtgtccg
     6001 ccggccgccc gcgagggagc gtcgtccgcg gcgaggatgg ccgatccggg cgctggatgg
     6061 gcggaagcgg gcaattgcca attgagctgc atgcgtcctc tcccctaggt gctgccgatg
     6121 ttctgccgca cgtgatcgag cgagacgtgc gagccactgg tgctgcgagc gtgccgctca
     6181 atccgttcgc gaagctgctt ggcggctgag attcgaatga gcacgggctc gcgatcgagg
     6241 tgtcgctcaa gctcgacctg ggcgtcctcg tcccaggaaa tggtcgccgc caggcgcgcc
     6301 ggcgtcggct cgatctcgtc gagcttggtg ccgagtggca ggatgttgaa caaggcatcg
     6361 aacatcgcgt tgcatacttc ctggacgatg tagacggcgc ccgcgtagcc catgaacggc
     6421 gtgcccgtat gccgccggat gaccgcgccc gggaacgagg cggggatgta gatcgaccgc
     6481 ccgccggcct cggcgagata catgcgctcg ttgtagcttc cgaacagcac gagcggcggc
     6541 gtctccttga gcgccttgcg taccagctcg ttgtcgggct tgacgcccgg ccgccgcgag
     6601 acggcgagcg tgcacggcag cccgagttcc tctgtcagga aattgcgcac gccccgcgca
     6661 tacgtctcgc cggcgacgat tccgtagctc gccgtgcaga agaaatcctg cgtcaccgac
     6721 cgccacagat cccacaacgg cttgatcgtg gtgtgcttct cgcgcgcgat gaagggctcg
     6781 ggatcgagac ccagatgctc gccgaggctg cgcaggaacg cggtagtgct gtgaaggccg
     6841 atcggcgcct gcagatacgg ccggtcgagg gcctcgcaga gcatgcggcc aaactcgcga
     6901 tagaggcaga cgttgacgtc ggcgttggcg agcttcggca cgtcggccag atgcgacccg
     6961 agagggaaga cgagattgac ctcggcgccg atgccctcga tcaggcggcg cacttccgca
     7021 aggtcgctcg gcatgttgaa ggtgccgtag ctcgggccaa tgatgttcac gcgaggcttc
     7081 tcgcccgctt tgcgcggctt tttcgccggc acgcgcttca gtccgtactc gctccagagc
     7141 cagctcatgg cgcggttggc gcactgccac tgatcctcgt cgatggtgcg cggcaggaag
     7201 cgctggatgt tggtgccctc cggcgtgact ccgccgccga tcatttcagc gatcgagccc
     7261 gtcacgacga cgctcggcag atccgggtcg aggctccggt gggcacgccg cattgcgccc
     7321 tccgtgccgt gctggccgag ctcatcctcg ccgaggcccg tgaccacgat cggcagctcg
     7381 tgcggcggca gcgcatcggt gtagtggagg accgacgtca ccgggaggtt ctcacagccg
     7441 acggggccgt cgatgacgac ctgcagcccc ttgatggccg tgaagacgta gatcgccccc
     7501 cagtagccgc cagcccggtc gtgatcgctg atcagcatca gaccatctcc tcggacttgc
     7561 gagcggccgc ctgcttggcg agcttgcgcc gataggtctc cttgaagtcg cggcggtcgg
     7621 cgggaacatc ggtccagatg cctgcggcgt ccccttggcc gacaccggtg aagaacgatg
     7681 tcatggcatc gaagcgtgcg cggttcgcga tagcggtgtt gacgacctgg gcgagcgaac
     7741 cggcaccggc gggtcccatg agcggtcgcg ccgagatcag gttcgtgaaa tagagcgcag
     7801 gaatcgcgcg ctgcttggcg tgctgcacca ccggtgtcgt gccgatggcg agatcgggat
     7861 cgaaggcatc gaccgccgca atgtcctgct cgagggaggc gcggtaggtg acggggacac
     7921 ctttcgactc gagccaggcc cggtccggct cggaatacgg tgtgcgcggg catgcagtgc
     7981 ccacgtagcg gacgtcagcg ccgctctcga cgaggagccg tgcgacgagc agctccgagc
     8041 cctcgtagcc cgaaagcgtg atgcggccct tgatcgggtg agccgcgagc acgcctcgga
     8101 tggcgggaag gatcgcgttc gacgcgcgcc cgacgacggc gcgcggcacg ccgtaggcat
     8161 caccgatggc cgcgatccag gccgccgtgc cggcatgacc gacgggcgcc gagcctacga
     8221 tcttgcgtcc ggccgtctcg aactcgcgga tcgaggccgt atagaatgga tgaatggcag
     8281 cggccacgcc gcagtcgagc gcggcataca gctcgcgcca ttcgcgtgtc ggcacgaccg
     8341 ggccggcagc aagaccgagc ggctcgagta gccgaccgat cacgatcggg tcggcgggga
     8401 acatttcgcc gaggagcgcc acgccgggac gctccgcgcg tccgccgcgc ggcgcctgaa
     8461 cgggtccctg gctgacctcg ttccgggcat aggccagcat ggcgccggcg agcacgtcct
     8521 tcgcctcggc aagcgtcttg agggcgctct nnnnnnnnnn gcgtcggcac accaaaaccc
     8581 ggcacgtcga tacatacgat ccgtacgccg ttgatctgct tcgggagcaa ttggagcggc
     8641 acgccggagg cggtcggcac gcagagattg atgaccacga ccgcgtcatt gctggcggga
     8701 tcggcgagct tgaagacggc ctcgcggatg tcctcgaaga gcttccccgt gaccagcgtc
     8761 tcgctgtcga acgggacgta gccgacggtc cgccgcgctc cgtagaagtg cgaggtgaag
     8821 gtgagcccgt agacgcagca ggcggagccc gacagcacgg tcgccgtccg tcgcatgcgc
     8881 agcccgacgc gcagcgagcc gaaggcgggg cacatgctct gcggcttgtc gtgggggccg
     8941 accggatagt cggccgcgta gcggtcgagc atctcgctct taccagaggc ctcggcggcc
     9001 cgcctgagct cgtccttacc ggcgtggcac gacaggttgg ccgtgcgcgc cgcgcctggc
     9061 tgctccgcag acggcgtttc gccgctctcg ggtgcgacca cctcggatcc tctgccggtg
     9121 ctgacgtcac cgttcgtcat agcttgcctc ccagaccgaa ccgcggctgg atcaaaccga
     9181 gtcgtagatg acctcgagcg acttcttctc gttggctgcc gcgcctcgca tgtccgcaat
     9241 ggacgccggc tcaagaacca cgccgcggcc gacggcctca ctcttgaaaa ggccgagcag
     9301 ggcatcgggg ctgagcggct tcgggcgcag tggcggggcg tccgccacgt tcgtcgccag
     9361 cccctcgaac acctgagacc agggtccgcc cggccggccg atgatctcgt agcttgcgct
     9421 cttgcggcgg atatcctcgt tggcggggat cgctgcaagg atgggaatgc cggccgcctc
     9481 ggcaaaggct tgagcctcgc cggtgccgtc gtccttgttc acgacgagac ccgcaacgcc
     9541 gacgttgccg ccgagcttgc ggaagtactc gactgcggag cagacgttat tggcgacgta
     9601 gagcgactgg agatcgttcg agccgacgac gatgaccttc tggcacatgt cgcgcgcgat
     9661 cggcaggccg aagccgccgc agaccacgtc gccgaggaag tcgagcagga cgtagtcgaa
     9721 cccccagtca tggaagccga gcttctcgag cagctcgaag ccatggatga tgccgcgacc
     9781 gccgcagccg cgaccgacct cgggtcctcc gagctccatc gcgaacacgc cgtctcgctt
     9841 gaagcacacg tcgccgatcg cgacctggtc gccggcgagc ttcttcttgg cggacgtctc
     9901 cagaatggtc gggcaggcgc gtccgccaaa gagcagtgag gtcgtgtcgc tcttcgggtc
     9961 gcagccgatc agcaggacgc gcttgccttg ctgggccatc atgtacgaca ggttcgcaag
    10021 cgtgaagctc ttgccgatcc cgcccttgcc gtagatcgcg atgatctggg tggccttcgt
    10081 cgggggcgca atcacgaccg gcgccggagc cgtcgccgcc tcgcgacgca gtcgatcgac
    10141 gcgcacctgc tcgtcgagca tcgccacagc cccgccgcac gatgccgtca tgggaacgcc
    10201 ctccaatcga gaaccatctt gaggcatccg ggatcctcga atgcctgcct gtaggccgtg
    10261 gccgcatcgc gtgccgcggt gacgtgagtg atgatgccgt cgagcgacag ccggccgttt
    10321 gcaacgagcc cggcgaccgc ggcgaggtcg gctgcctgcc actgggcggc aacccgcaga
    10381 cgcagctcgc gcatgaacgc cggcggaaat gcgaagctca gcggctcctc gtagaacccg
    10441 gcgagcacga tctcgccgcc gggggcgagc cggccgacca gctcgtcgag cacgccggtc
    10501 gagccgctga cgtcgcagat gcgattgtag ctgcggcagc cgtcctcgct cggatcgatg
    10561 accgaatatc cgctggcgcc ctcgcggcga ccggcatgcc gctcccatac gaccggtggc
    10621 ggccccccgg cgagcatcgc aagacgggcg agaagacggc cgagagcgcc atggccaacg
    10681 atcagctccg gcgcgggctg cccgagcggg gccgcatgat gcgcggttgc tgcgagcgca
    10741 aggagcgccg actgctcggc gagcggctcc ggcacctgca cgacacggct gctctcgaca
    10801 acgagccgtt gagctgagcc gccgaagaga ttgcgaacgt cggtgaatgc ccgggcgccc
    10861 ggcacgaaca cgcgagcacc gaccgcaatt ccggcgctgt ccggagcgga ggccacccgc
    10921 cccacggtct cgtagcccgg caccagcgga tatcccatcc cgggaaactg cggcatacga
    10981 ccggaccaga acaggcgctc cgttccagcg ctgatcccac tccactcgac atcgatcagg
    11041 aggtcgccgt caccgggcgt gcgcagctcg gctcgatcga ccgaaagcgc gccgggccct
    11101 ttcatgatga cggcggcggt ctgcatcgtg tcgctcgctc tgccgattca atgactggat
    11161 cgcctaaggt ccaggcgaca tcacgtagca tgatgttgac tttacgttat gtcaaatatt
    11221 ttttacgttt gggcgtcatt tctcatcaag ccgaggccac gagcacgctc gcgatcatcg
    11281 gctgcgccgt cgcggctttc cgaaatgagg caaagccagc cgctcgcagc aactcctcca
    11341 actgccctgg cgatcgtgga cggccgctgc ccatcgccag gagataaaag gcgaaataga
    11401 catctgaaac gcgctctgcg ccccggatgc ccgacatggg ctcggcgatc acgagcgcac
    11461 cacctggcac cagggcgcgg cggatgtttg cgaaaatttc gacagcgcga tcatcatcat
    11521 ggtcgtgcgc tacgcggatc aaggtcgcag cgtcgaagcc ggtgggaagc ggatctcgcg
    11581 cgaagtcgcc tccggtggtt gaaatccggc ctccgagacc gagcgaggcc aaacgcgact
    11641 cggccagctc taccacggcc ggcaaatcga acaggtgaag cgccaggccc ggatgacgtg
    11701 cagaaagagt cgcaagaaag gcgccctccc cgcccccgac gtcgagcacg cgacggtggc
    11761 gatggaaagg ataggcatcg atgatgttgt cggcgagcag actctgcgac gtcgtcatga
    11821 gccccgaata gggctttgca caatcgggct tgatgccatg cggcgctgcg ctcgccgcgt
    11881 atggccagta gccggcgagc cttgtgtcgg cctttgggcg tgcgagcagg gcaacggggt
    11941 cggcgaggtc ggcataaagc atggcgtgat gctccaccat cgccgcgatg ccaggattgg
    12001 cagagatggc ggcgccgcgc atcccgagcc cgatccgccc gtgactgcgc ctcgaaagca
    12061 ggccgagggc gatcgccgca ttgatgaggg tccgcgccga ctgctgcggc aatcctgtac
    12121 gcgcgacgag cgcctcgagc ccgatcggtc cgtctgccac cgccttcagg acaccgagtt
    12181 tcacactggc aaagagcgtc tgcgaataga cgaatcccgc acagaggtcg aaagccgcct
    12241 gcgcctcgcg cgcggccttg cctcgaacca gcggcatgcg ggcggcccat ctctggaacc
    12301 gcgtgctcat cagcaggcga tccctgagtt cgagcaggcg atcgacgaga ccggaccgcg
    12361 cggcggcggg caattgatgc cggtcgaccg aattcccttg cactgatcgc tcctgcgccg
    12421 ctgcgggacg gcgagtgctg tcgtcgtcct cacgcactcc ttgaaagccg ccaagtgacg
    12481 acatcgtgta aatttcattt tgctcttctt tatgtccgaa atctacgaca atatggtgcg
    12541 tcgctcagca ggatcgcgga atgagccggg atagggtcgt cgttgtcgga gcaggcgtcg
    12601 gtggccttgt cgccgccatg atgctcgcgg cccgcggcgt cgaaactgtt cttgtcgaga
    12661 agcagacaac cgtcggcgga cggatccgcg agttgactgt ggcgggccga tcgatcgatg
    12721 cggggccgac ggtgctcacg cttcgccacg ttttcgatgg gatcatgacg gcctgcggcc
    12781 tcgacctcgc cggccttgtc cgcctgaccc gcgccgacgt cctcgcgcgc cacgcctgga
    12841 gccccgaaga gcgcctggac ctcttcgccg atcgcctgag atcagcggat gccatcggct
    12901 cgtttgccgg cgcggcagcg cggcgcggct atctcgactt ctgcgcgcac tcccggcgca
    12961 tctatgagac catggacccg atcttcatgc ggagcgagcg cccgacgccg atcggcatgg
    13021 tgaaggcggt cggcctcagc gggctcggcg gcctcatctc ctctgccccc ttcacgacgc
    13081 tctgggatct gctcggccgc tactttcccg atccacgcct tcgccagctc ttcgggcgat
    13141 atgctaccta ctgcggatcg tcaccgttcc tcgcgccggc gacactcgct ctcattgcac
    13201 acgtcgagca ggacggggtc tggctggtcg aaggcggcat gcgcgctttc gccgatgccc
    13261 tcgccgtggc cgcccgtacg aaaggcgtcg aaatccgcac gggcaccagt gtcgccgaga
    13321 tcctcatcaa ggatgggcgc gcccgtggcc tccggctcgc gaccggtgag acgatcgaga
    13381 cggcagccgt cgtctccgcc gtcgatgtcg gcgcgcttgc ggacggcctg ctcggtgccg
    13441 acgcgcgctc ggcggtagat gggggcgcct cgacgccccg ctctctctcc gccgcgacct
    13501 ggggcctcgt cgcgcgcgtc tcgggcctga cgctcgcgca tcacacggtg ctcttctcgc
    13561 gctcctcgcc tcaggnnnnn nnnnncggcg tgccgccctc ggacccgacg gtctatatct
    13621 gtgcgcagga tcgctcggat tcaggcgagc gccgcgccga tgccgagcgg atcttcatca
    13681 tcaccaacgc tccggccgac ggcggccggt cgctcggcac acccgaggag cgagaggcat
    13741 gcgagacacg gatgatgggc acgctggcgc gctgcggact gacgctcatg atcgacggca
    13801 tggtccacac ggggcctgcg gagttcgggg cgctctttcc gggcacgcaa ggagcgatct
    13861 acgggatggc gtcccacggc tgggcggcat ccttcagacg tccggggacc cgctcacgca
    13921 tccacggcct ctatctcgcc ggagggagcg tgcatcctgg nnnnnnnnnn nnnnnncact
    13981 ttcggggcga gcggctgcgc tgaacctgat cgcggatctg acctcgacac gccggtagcg
    14041 tccggtggct acgcgtggtg gtacgtcgat gcactgagcg acgacggccg cttcgggctc
    14101 agcctcatcg ccttcgtcgg cagtgtgttc tcgccctact acgcgcatgg ccggcgacgc
    14161 ggacgagccg acccggagaa ccacgtcgca atcaacatcg cgctctatgg cgaggggggc
    14221 taccggtggg caatgacgga aaggggccgt cgatcccttt cgcgcactcc caacctgctc
    14281 cagatcggcc cgagccggct cctccgcagc ggcagcgcct ggcatgcgca gctcgacgag
    14341 atctgcgtgc ccgtgccgcg ccgcatccga ggctcattca cggtcgagcc cctggcgtat
    14401 ggccggcaaa cgtacatgct cgacgattgc ggccggcaca tgtggcggcc acttgcacct
    14461 cgcgcgcgca tgacggtcat gctggaggac ccgcgtctca cctggtccgg acctgcctat
    14521 gtggaccaca accggggaac ggagccgctc gaggcggctt tcagtcgatg gtcatggtca
    14581 cgctccgtgg agaccgatcg gacggtcatc ttctatgatg ccaaaatgcg ctcaggtgct
    14641 tcgaaggagc accatatcgc cgtcggggca gacggagcgc cgacgccgct tgccgcaccg
    14701 cccatggcgt cgcttggcca gagcctgtgg cggctgcccg tcgaggtccg caccgacgct
    14761 ggttcgacac cagagcgaat cgcaacgtgg gaggatggac cgttctatgc ccgcacgctc
    14821 gtccgacacc ttgtcggggg cacgccggtg acgtcgatcc acgaggcact gtcgctggac
    14881 cggttcaaga gacgcacggt gcagatgctg ctgcctttcc gcatgccgcg ccgaagctga
    14941 actgctcgcg caatgcctgc ttccacgcac cccaaggtca ctgcccatcg gacgattggt
    15001 tcaatctgcg atagagatcg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcctccac cggcgagcac ccgacacgac
    15181 caggagagcc tcgatggcga ggacggcaag cgcgatatcc gcgatcacgg tcgatcggaa
    15241 caggtcgccc atgcttgctc ctcagccttc tcagctcgca ttcactcaga atggcgcgtt
    15301 cagacgcacc actgcggcgt tgagcacggc cgcaaacgag acccagacca gatacggaac
    15361 cagcaacagc gccgccggac tggagcggct tcgtgccatc cagacgagaa ctgcaatcga
    15421 gagccacaat aagccgacct cgatcagcgc cagatcaggc ctgcgcaggt aaaagaagag
    15481 cacgctccag gcgacattca gcagaccgtt gaggaccagg gccgacaccc agcgacggcg
    15541 gcccgcgttg tccgggccgg ctcgccaggc catcgcgctc gcgatggccg tgagcgcaaa
    15601 gatcagggtc caagccggac caaaggccca gtccggcggc tggaaccagg gctttttgag
    15661 agcccagtac caagggccga tcgtggtcgc gatgccaccg agaaccgcaa cccctgacca
    15721 gccggcggcc gaggcgagca gcggcttcca gagcgcccga tcaccacgat catgcacgca
    15781 cgagacccag cagatgagcg aggtccttga agaagattcg aacatgggca aagggctttg
    15841 cccggacgag acgcttgcgc atgtaggcct cgaaggtcag cctctgcacg tcgggatcgc
    15901 ggcagatggt gacaaaccgc tctcgacggc gatctgatcg ataccagaag tactgcatca
    15961 cgcccaggac ccagaagacg cggccgtgca ggcgcatgaa ctgccgccgc gccgatctga
    16021 gcgcgcgcgc atcccccgtg gtgacgaact gtgccgctgc ctcggccgcg aaccgtccgc
    16081 cgagcatggc gtagtagatg ccttcgcccg aagccggcgc gaccaccccc gcggcatctc
    16141 cggcaagcac gacatttcgt ccgtcgtccc agcgcctcag cggccgcatg gggatcggtg
    16201 ctccctcgcg gcgtacggtg cgctgcccgg caaggccata gtggcgacgc acatcggcga
    16261 cggcctcgcg caatgcgaac cctttcgacg cgctccctgt gccgacgctc gtcgtgcggc
    16321 cgtgtggaaa gatccagcaa tagaaatccg gcgacgtcgt cccgcgatag atgacgtcgc
    16381 agcgcgcgct gtcgaaccct cgcgcgggct ccttgacgtg gttttcggtg ttttcgggag
    16441 actcaatgat ctcgtgatag gcgaagacgc accgtgctcg ctcgacgtcc ggcatcgcaa
    16501 cccgtgcgac gcgcgagatt gcgccgtctg caccaatgac cgctcgggcg cgcagccggc
    16561 ggagcgagcc gcctgcatcg tcgcctgcag cgcgatagag cacggtcacg tcaccgtctg
    16621 cctcgcgcac gagatcctcg aatatccctc cgaggagaac cgccccggcg ctggccgcgc
    16681 gctgtctgag ccagtcgtcg aaaacctcgc gatcgaccat gccgacaaag ccgttctcaa
    16741 tcgggatatc gacatgcgcc tcactcggcg ccaccatgcg cgccgatgta atccgagcca
    16801 cgagcagctg ctcagggatc tcgaactcct caatgagctt cggcgggatt gcgccgccac
    16861 acggcttgat gcggcccatg cgatcgagga gcgccacccg gcagccggcg ctcgcgagat
    16921 cgtgagccgc cgtcgcgcca gaagggccac cgccgacaac gacgacgtcg aatgcagtgt
    16981 cgatcgtcat gatgctgcac ctgcacgggg aacatctgcg actgttgggc ctgcccgggt
    17041 cccggaggtc cagatccaat cccgggggcg gcctcgggcg ccgtactcgg tgagcccgaa
    17101 cgcgagcacc gccgacgtca cgaagagcgc ggcctcgatc ccgaagacga gggcgtaagc
    17161 gtgcgatggc gagctgacca ggccgcgcgc cacatcgatc atgaccgtac cgccgaagcc
    17221 gccgaggccg aatgcaattc cctgcgcggc accccagaga cccatgcgcg tgccttcccg
    17281 gccggccctg ccctcgcctg ccatgcgcat catggtgccg atcgctgcga tggcaaaggc
    17341 gccattggca atgccgagca cgacgaccgc cgccttgagt ggacccgcgg gacccgcaaa
    17401 ccccgcggca atgaccgcta tcagcgctgc tgccgaggcg aggcaaccgc cgatcgtcca
    17461 caaacgcaag gaaccgagcc aacggccacc gatggcagtc gcgcagaccg caaccagcac
    17521 catgccggcg aagacgccgc cgtgatgcac gccagacagc atcgtggact ggcccggcgt
    17581 gtagccgaac aacagccccg cgaacggttc gagaatgagg tcttgcgtgc tgtaggcgag
    17641 catcgagacg aagatgaaga tcgtcagccg ccgcgcctgg ctgtcgaccc agacctcggc
    17701 gagagccgcg cggaattcag gcatcgcggg ctttgcttcg gcgtcgcctg tcgtcgtggc
    17761 cagggcacgt ggttcgatgc cggcaagtgc cgctatcgcg accaggacgg cggcgacacc
    17821 gacaccggac gtaacgacga tcaggcggcc gaacgaaaac ggctcgagga gccggccgac
    17881 cacgatcgcc gtcacggcga agcctgcgat catcatcagc cagacgatgg tggccgctgc
    17941 ggcgcgacga tccttcgccg tctgcttggc gagcagcacc agcaggttcg ttccggccgc
    18001 gctgacgccg aggccgatca ccaggaaggc aatcaccgcg aggaccaggc ccgggacgaa
    18061 gctcacggac atcaccgagg tcgcagccgc ggccagaacg gcgccgagcg cgagcaccat
    18121 catgccgcca acgatccacg gcgtacgccg tccgccgaca tccgagccgt agcccatgcg
    18181 tggtcggagg atctgcacag catagtggag cgcaaccagc gcccccggga tcatcgccgg
    18241 aagggcaagc tcgatcacca tcaggcgatt gagtgtcgag gtcgtcagca cgacgattgc
    18301 gccgagggcc gactgcacga gaccaagccg cactattccg agccagccaa gtccgaccac
    18361 tgctcgttcc gtcattgcca tccccctgtc aagatgccgg gagcgatcgg atcgccgccg
    18421 ccgcaatcat catgccgagg accagcagtc caatgccggt cgaattgtac caaggtgcgt
    18481 acctccgcgg atcgctgagc aggcggcgca tcaggatcac ttgcagcaga cagatcacga
    18541 cgatcgcgat gccgtgcccg gcccgccccc acgccatgag aagcgccgcc acgaccagct
    18601 gcggcacgat catgacgact gaggcagtgc gggcagcgaa ctccggaccg agcatgacgg
    18661 gaagcgagcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc catggccagg atggaagtcg
    18781 acggcatctg gtcgagcatc aacgctgcgc ccgtcaccca ggcgagccct tcgtagcaga
    18841 ggccgcaggc tgcattaccc caccagccgt ttctcttgag gcgcagtggc ggagcgctgt
    18901 aggcccaggc catgacgagg ccgaggaccg tcgcgcccag cacgacgcgc ccaagcacac
    18961 tcgccgctcc gagggacaga aggcaccagc cagcggcgat gtagaggccc catcgaccgg
    19021 ggatccgccc cgatgggata gggcgcttcg gctcattgat cgcgtcgacg tggcggtcat
    19081 gccaatcatt gatggcctga ctagctgcac agacgagcgg gccgaccagg actattccca
    19141 ggagaattct gctccacgat ccgctggcgg gcatcccgga cgagacgacg ccgcaggcga
    19201 atgcccacat gggcgggaac cacgtcaccg gcttcaagag ctcgatgacc gcggctatcg
    19261 aaggaacctt ctcgttggtc aaaacaatca tgcaccaatt attgtctatt ttcatttact
    19321 gtcaactcga gacctcatgc ggcacggcac cgagaggccg cctgccggaa gcccggccga
    19381 tcactcccgc tcacgcgacg tgcctggatc ggcgaccgcg gtcgcggccg cttcccggtc
    19441 tcgcttcagc tcccgccgca tcttgatcac ctgccagatg ccgacaccga tcgacagcgc
    19501 ccctacaaag acgaactcga tcaggccagc catgctttcg aatgtcatgg gagtctcctg
    19561 gtcgggttcg ccgacgagcg gcctgcaggt cgcgacagca tcgaaggatg tcaagcgctc
    19621 ttggaatggt ccgatccgcc gccgaggaac cggcgcgcgc ccctgacacc gatcgcgacc
    19681 agtgctgcga ccagcggaac gctgatggca atcaacaggt ccgcgcggcc aacaaggccg
    19741 gcgaccgcag cacccttgaa caagtagctc aggagaccga ccagatagta cgttatcgcc
    19801 gcaaccgaga gcccctcgac cgtctgttgc agccgcagct gtagcagcac gcgttggttc
    19861 atcgattcaa ggacacgttg attgtgctgc tgacgcgaga cgtcgacccg agttgagagc
    19921 aactgcgtcg cctgcgcgat gcgcagtgcc agcttctcgt gccgcgcaga tgtcgccgtg
    19981 atcgtactca tggccggact gagccgacgt tcgacgaact cacgaaacgt ctggagaccc
    20041 tcgacgcgct tctcgcggag ctcctccgtg cgtcgctgga tcagccggta gtaagcctcc
    20101 gcggcgctga agcgggacgc cgtctcaaag tgccgcttct ggatctgcgc ttcgagcctg
    20161 gtcaggcggt cgagcaatgc tggctcgtcc acggccgcaa catctgccat ggctgcagtg
    20221 atatcgacga gatcgcgctc ggccgtcgtc agagagggcg atagctcccg cgcgatcggc
    20281 agcgccagca tcgccaggat gcggtaggct tccatctcca gcagccgttg gaccgtccgc
    20341 cccgcctggc gcggcgtcag gttgcgatcg agcacgagga ggcggctgaa gccgtcatcg
    20401 cgtatccgat agtccgtaat cgccagcgcg ccaccgtcag cgacgaggga gccgacgatc
    20461 gtgttgccga tgaagtgctc gccggcaagt ttctccacgt ccggtgtctc gcggccgaat
    20521 gggagaagcg cgacatgcgt ggcggagatg atctcgcccg gcaagctact cagccattcc
    20581 tgcggcacag actgcagcgc cggctcagcg aacggtgaag ggctcgtccc gccgatgata
    20641 aacttgtagc gtgtgaactc ggtatgcttc tcccacttga cgcggaactt gccgcaatcg
    20701 ccgctgaagt ggctgacacc cgccggcggc ggctcgatca tgtagcgctt gcagagctcg
    20761 cggagcggcg cttcgtgctt cacccgctcg atgccggcat catagagcgc gagaaaggtg
    20821 atgcgctgcg gtgcccgcaa cgcctcgggc gggcgggcat gcgcctcctc gttcagggcg
    20881 cgccgtagcg ggaactcctg gggcagcggg ctcaaaggtc gctgtgcctc cctgttgtcg
    20941 atctgagggg ttcttcacgg ccgcagcttg caaggcccgc gatggcgccg cgcacaatgg
    21001 cggcaactgc tgcgggctgc tcctcgtgcg cgaggtgccc ggcatcgcgc agatagtgca
    21061 cacttgcccc ggcaacgagg ctcttgacgg tccacgccgt ctcggccggc acggcccggt
    21121 catcaccgca cgcgaccaga tcgagcggcg cacgaagccg gggcagctcg gccgaaagac
    21181 gctccagtga ccagttggcc atcatgtcga gagctgactg aacgtgcgcg ggctgcttca
    21241 ggagcagccg atagaacccg atgcttcgct gcggcggctt tccgcctgtg ccgatcagca
    21301 gacgctcgac ggctccagcc cgctcggcgt gccgtgcgaa gaggcgcgcc gtgaacgggc
    21361 ccgcagcgag gagcctcgcc atgggcggaa aaagccatcg gccaaggccg ccgaacggca
    21421 ggaatgcgcc gttgatggca atgatccggc aggagcgcgc gagcgagccg tcgagcgcca
    21481 tccgcacggc gacagctgct ccagccgaat gcgcaatcac cagctgaggt gcaatgccga
    21541 ggacagacac caggcgtgcg aggctgcgcg cgtacccctc cagtgacatg tcgctcgaag
    21601 ctgccggcgc gctgaagccg tgcccaggaa gatcgacgag cgtgaggtgg agatcggccg
    21661 cgagctcgtc gacaagacct tcccaggaat gggtcgaagc gccggtgcca tggatgagca
    21721 gagctggcgg gccacgcccc atctgctgga tatggaccac gcggccttcg atgtcgacga
    21781 gacggctcat ggtcgcgttc ggccagatca cgccatcgag gctgaaccgg gtcgagttca
    21841 caaaatcatc cacccgagca tcggtcgtga tcgccatgac gtcgccgtcc tgcgagtgtg
    21901 cctcggcccg catcatgcgt gagcgctttt cttctggatc tcctcggcga tggcgcgcgc
    21961 ttcggcatgg ggcagcggat tgtacgacgc gcccatcagt cgggccagcc ccgcggcgtc
    22021 ggcgttgccg cgcggtgagg tatctatgat gaggctcgcg tggccttcgg cccgcaattg
    22081 gctagcagcg ctctcggcat cgaggagggc cagcttcctt ccgggagcgc cacgccggct
    22141 catgttcgcg cgaccatccg tcagaatgac gatcaagcaa tcatgtccac tccgcctttc
    22201 aaggctcgcg agttggcacg cggcatcgat cccgcttgcc aacggcgagg cacctccgcc
    22261 c
//
LOCUS       DAORNH010000035        21947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1840_length_21947_cov_2.653618, whole genome shotgun sequence.
ACCESSION   DAORNH010000035 DAORNH010000000
VERSION     DAORNH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21947
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1840_length_21947_cov_2.653618"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="PK264_05795"
     CDS             <1..1615
                     /locus_tag="PK264_05795"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75438.1"
                     /translation="PWFMPMRRPGAAAAQERLKFKWVDDTLSGLRIGVPFALVKEVPE
                     SRSWNGHQWQSEDGQTLITTFKHPLSKYTINSYFRTMLGGRPNSRIAHIDLTSESFVI
                     EGESVGPKGPYSYQVRAYQVGDHIVGMLAHYPPAVADDMERVVNANASRFQRNNGWRT
                     IYLSNCGSKQPGEARIFYGTNRAQLTPATVAADGVDIDGLFAPIPAGQLHLGCAHVSV
                     PTDIEARRALTPSPGLASANPTAHYILKRFRPITSSAVGDPGRRIVLVDDTYAEGGER
                     ALIFIHGYNVGFGDAVRRMAQIASDTGYKGKIYVFSWPSLGQRTRYTADLDTAEQSEA
                     YLESFIRLILTDRNVQTLDVIAHSMGSQPFLRVVRGVARSFDQPNRRGRRIRFGQVIF
                     AAPDVGAEVFKEKVREIVPLAKRVTLYASSTDCAMYASRLLRLGRGRAGDLENGKPLV
                     IDGVETIDASDVRPSGLLERFSTVVNWGRLRNYFSCNHAYFADRQKLLDDLTALLRTD
                     DAPPSRSSATMTRIGPSDSRSGYWRLPPE"
     gene            2940..3221
                     /locus_tag="PK264_05800"
     CDS             2940..3221
                     /locus_tag="PK264_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75439.1"
                     /translation="MDHRTLLDPTGEHEAVAKKLAARPRSLDGVTVGILDIGKARGDV
                     YLNQLALRLGERGIAVKRYAKPTPSRLASGETRARMKSEVQAAIIGLAD"
     gene            3243..3461
                     /locus_tag="PK264_05805"
     CDS             3243..3461
                     /locus_tag="PK264_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75440.1"
                     /translation="MHDGSYLDGTGIPALIVATTAFVQAADVQSKALGYTPTITWVPH
                     PVQDRTDLELQRLADDTVDRIVQGLTTA"
     gene            complement(3523..4068)
                     /locus_tag="PK264_05810"
     CDS             complement(3523..4068)
                     /locus_tag="PK264_05810"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HRD75441.1"
                     /translation="MRSSRQLPPTVILLMGVSGAGKSTTGKNLGRALGWPFRDADEFH
                     PAANVAKMRSGHPLDDVDRAPWLAAIAAYIDERRAKEEPAIVSCSALKRRYRDVLLDG
                     RPDVALVYLFGTRQMIGERLQRRRGHFMPATLLESQFAALEEPQPDERPLVVSVGLSP
                     RRVVGSIARGLGLDLGAGSRR"
     gene            complement(4079..5080)
                     /gene="gnd"
                     /locus_tag="PK264_05815"
     CDS             complement(4079..5080)
                     /gene="gnd"
                     /locus_tag="PK264_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRD75442.1"
                     /translation="MQLGMIGLGRMGANIVRRLMRGGHTAVVYDRDPKLGTVLAAEGA
                     VAADGLADVVQRLAAPRTVWVMLPAGSATEAAIGELAALLSAGDTIIDGGNTNWKDDI
                     RLAAMLSGKGLHYLDVGTSGGVWGLERGYCLMIGGAKEVFERHDPIFATLAPGAGSIP
                     ATPNREARDPRPAHGYVHAGPNGAGHFAKMVHNGIEYGMMQAIAEGFDIMRGAAGPTL
                     PAEHRLALDVADIAEVWRRGSVITSWLLDLSAAALAEEPGLESYSGHVEDSGEGRWCV
                     EAAVQEAVPAEVLTAALYARFRSRQGHTFAEKMLSAMRKGFGGHVEPKRPAAQKSAT"
     gene            5441..6625
                     /locus_tag="PK264_05820"
     CDS             5441..6625
                     /locus_tag="PK264_05820"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD75443.1"
                     /translation="MRQPLSGIRVIDLTRILSGPFCTMMLGDMGADVLKIETPGGDPV
                     RGHGDIVEGLSWYFAAFNRNKRSVVIDLRKPEGKEVLARLLAKADVLVENFRPGVLTE
                     MGFDQARLDVLNPRLIVASINGYGSVGPYVDRPSFDFIAQAMSGLMATSGEPEGAPMR
                     TALPITDLVAGLYCAFGIVNALRARDLNGRGQRVEAAMVNGIVSMLAYLASEHLATGR
                     NPQRTGNDHPIAAPYGLYRAKDGEIAIAPSTNESLEKLMRTLGLSEMLERPEYRTPQL
                     RHARRHDFRAPINAALAVETQDTWITRLNAAGVPCGKIMTLKEVFADPQIKAQQMVLE
                     IDHPGHGVVRTVGFPVKLSGTPCAVRLPVPELGGHTDEALRESGLADAEIAKLRAAKV
                     IV"
     gene            6634..7872
                     /locus_tag="PK264_05825"
     CDS             6634..7872
                     /locus_tag="PK264_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRD75444.1"
                     /translation="MASNLQINADRLWSTIMASAEIGKGPRGGLCRLTLSDDDRTVRD
                     LVAGWANVAGWSLTVDRLGSMFIARRGREPSLPPVLIGSHLDTQAAGGRFDGILGVMA
                     GIEVLRTLDDLGLETRRTIEVVNWTNEEGARFQPPMLCSLAFAGKAAADWVLDRIDKE
                     GRRFGDELARIGYAGSAAVGGRAIDAYFELHIEQGPKLDAAKVPLGVVVGGFPTAGMR
                     IEIRGRTSHVGPTPMEHRRNALVAAAHVAVAVDEIGWRYAAGEGKTTAARLDLWPNLP
                     GILSERATLYCDLRHPDASALERMLAEVATAIPDCARRGRCEIEIAERWGFGGFSFDR
                     DLIALIRASAARRGVATLDLKSEAGHDAYHMASISPAAMIFTPCKGGISHNEEEDMTL
                     AEAVPGASVLLDAVLERANR"
     gene            8019..10079
                     /locus_tag="PK264_05830"
     CDS             8019..10079
                     /locus_tag="PK264_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD75445.1"
                     /translation="MPESHIPPDDDPFLWLEEVEGERALAFARAETAATKAALMDEAF
                     ARDREIVRAIMTAPDKIPFVSARGDRMYNFWTDADHVRGLWRRTTLESYRSPSPDWET
                     ILDIDVLNKTEGANWVWAGCHALGPQFERGLVSLSNGGSDAAVVREFDLEAKAFVPGG
                     FALAEAKSSLDWLDPDTVLVATNLAPAKQTTSGYPREVRRWRRGDPFERSEVIYACDE
                     TDMGTWVMVERGPGPTLVAIHRVIDFFSREVFLWREGAPLVRLDLPRDAEPLIRADRL
                     VITLKSDWAAGGWSFAKGSVIAISLARFLAGDREFAVLFEPTPRSSVQALLWIRSGIV
                     LTVLDNVQSRIEIAREAGDRWRVEPLAGLPAGTTTSTMAFEADDAASTDRFFVVANGF
                     LTPATLYLAEAGTLPVRLKEASARFDPSGLTVRQHEAVSSDGERIPYFEVARDGLAPD
                     GSSATLIQAYGGFDLSMLPGYQATTGRLWLERGGVLVVANLRGGGEFGPRWHEAGRLA
                     GKKQSHDDLAAVAADIARRGVSRPERIAAVGGSNGGLLVGSMLTRYPERFGAIVCGVP
                     LLDMRRYTKLPPGASWIAEYGDPDKPEDWAFMQAFSPYQQLAAGRPYPPILLHTSTRD
                     DRVHPGHARKFTARLRALGYEPVFYENEDGGHAGAADHEAVATRVALEYAFLRRALRL
                     AVPA"
     gene            10076..11317
                     /gene="hemW"
                     /locus_tag="PK264_05835"
     CDS             10076..11317
                     /gene="hemW"
                     /locus_tag="PK264_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRD75446.1"
                     /translation="MRASSEGSAGFGVYVHWPFCASKCPYCDFNSHVRAGGIDEARYL
                     RAYLREIEASAAETGPAGARPVVESIFFGGGTPSLMSPSTTGAILEAIGRAWSVASAA
                     EVTIEANPGSVDASRFAGYRAAGVNRVSLGIQSLRDADLKSLGRRHDVAEARRAIAVA
                     AATFERMSFDLIYARVGQTVAAWREELAEALTIAGSHLSLYQLTIEPETPFAALHAAG
                     KLPVPDGETSLTLYEATQEVMERHGLPAYEVSNHAAPGEECRHNLLYWRYGTYAGIGP
                     GAHGRLMRGPGRIATVGERNPERWLARVVEAGLGWTEATALTPEEQADECLLMGMRLT
                     EGIDLARLADLAGLAADRRQLEALARHGLITIDSGRDARASLIRATSAGRLVLNRVIP
                     AISETLAPIAAATTHKGTARP"
     gene            11314..12390
                     /locus_tag="PK264_05840"
     CDS             11314..12390
                     /locus_tag="PK264_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRD75447.1"
                     /translation="MTNPVLVELTRGALVESQHRGAVAVARPNGDLVVAMGDVARPVF
                     PRSAVKSLQALSLIETGAADRYGFGAHEIALACASHSGTEAHSSLAADMLARAGLTDA
                     ALGCGAHMPGHEATAHGKIKAGRAPGPLDNNCSGKHSGMLATAVHMKEAVASYLAPDH
                     PVQQRILKNMREVTGAALADDVRGIDGCSVPTWAMSLQTLATAFARLGSGEGLGPTRR
                     ATVERIMASCWEAPEMVAGPGRLDTAILGRLKGQVFSKTGAEGVYCAALVDRGLGLAL
                     KVEDGTPRAAQAALAMIIERLYPSVGTGRSAASRIGAAPRSARRGARTSSSRRSTDSR
                     RCGKDPPVSVMAGRVAGCDHMPRA"
     gene            12465..12767
                     /locus_tag="PK264_05845"
     CDS             12465..12767
                     /locus_tag="PK264_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HRD75448.1"
                     /translation="METEIAATEAPSNTDGSFEIYLAWQDVTLTVRGEQAAIDVLVQA
                     GVPIEPGCSVGGCGQCVTRYVEGDVIHKDACLNDEERKTMFCPCVSRARSRIVLAL"
     gene            complement(12780..13781)
                     /locus_tag="PK264_05850"
     CDS             complement(12780..13781)
                     /locus_tag="PK264_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HRD75449.1"
                     /translation="MTSLPDRMTAIVIDGKGGPDVLVPREMAVPEPGPGQLLVKVAAA
                     GVNRPDVQQRLGAYPPPKGHSPLPGLEIAGEVVAVGPECTRWRVADKVSALVNGGGYA
                     QYCIAEETSALPIPAGLGMIEAAALPETHFTVWNNVFERGGLKAGEWFMVHGGTSGIG
                     TTAIQLAKAFGANVIATAGSEEKCAACRALGADRAVNYRTEDFVAVVKEVTGGRGVDV
                     TLDMVGGSYTEKNLVCAAEDGRIVQIATLEGAVVELNVARLMMKRVTLTGSTLRPRTR
                     AVKAGFARALEERVWPLIAAGRVKVVMDQIFPLAHAADAHRRMESSGHIGKIVLTVG"
     gene            complement(13800..14597)
                     /locus_tag="PK264_05855"
     CDS             complement(13800..14597)
                     /locus_tag="PK264_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD75450.1"
                     /translation="MKAQINGIRMNFEVAGPERGPVVVLHHPLATNLTTWDELTAALT
                     HKYRVIRMDARGHGVTEASPAPYAFETLAKDVVALMDHVGARKARFLGLSMGGMVGQY
                     LGLLHPERFACLLLVSTSSRVPPEARALWDERIAAVRASGMKSQVQGALQRWVSPEAL
                     KSNKPLVARFTRMIEATPAEGYCGWGGAIRELDITGRLGEIRLPTRIIVGALDPSTPV
                     AAAEAIHKAIPGSDLVVMPGVSHMLHGEAPAEFHKQVIPFLDRHTRD"
     gene            complement(14696..15214)
                     /locus_tag="PK264_05860"
     CDS             complement(14696..15214)
                     /locus_tag="PK264_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRD75451.1"
                     /translation="MRQVAAKAAGPRAVGSYVPRLTRPSFEKFGFSAATLITDWCAIV
                     GPTLAGYTTPDRLRWPRLPAKIAGSDDEATGRPGATLFIAVDPARALDVEYQRAQIID
                     RVNRYFGYSAIAEVRIEQRYAKTSPPQRAAPVRPQKPIDILPLSTVADDGLRAALQRL
                     AEGLTNRLIVKT"
     gene            15396..16532
                     /gene="mutY"
                     /locus_tag="PK264_05865"
     CDS             15396..16532
                     /gene="mutY"
                     /locus_tag="PK264_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRD75452.1"
                     /translation="MAVDASIGAAEKPRPSRRQAPERGAASVGRRLLAWYDLERRELP
                     WRYPPGQTADPYRVWLSEVMLQQTTVKAVAPYFEAFTRRWPTVGALAAAHLDDVLTAW
                     AGLGYYSRARNLHACAKVVTADHGGVFPSREAALAELPGIGPYTAAAIASIAFGRRAT
                     PVDGNVERVVARLFAIERPLPAAKTELAALASTLTPQRRAGDFAQAMMDLGATVCTPR
                     RPSCLMCPLATGCKAREAGLAERLPARAAKAERPTRFGIAFLALREDGRLLLRQRDGK
                     GLLASMIEVPSTSWVDTWPAEDEALQAAPVNASWWRVPGVVTHVFTHFRLELLVYRAI
                     VPVDAQLTFWANAPRCRWVSRRALGEQALPSVMTKLIAHAMKDG"
     gene            16569..17489
                     /locus_tag="PK264_05870"
     CDS             16569..17489
                     /locus_tag="PK264_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD75453.1"
                     /translation="MLAYVLRRLLTAIPTLFIVVTISFFLIRVAPGGPFDLEQPLEAK
                     VMENLRRIYQLDRPLIEQYGRYLAALLRGDFGPSFYYRDFTIAELLRRGLPVSMLLGA
                     LALALALVIGTALGALAAFRQNGLADYAVMSLATLGLTVPGFVVAPLLQIVFGLMLAW
                     LPVGGWNDGAVRNLVLPVLVLALPQVAIIARLTRATMIETLRSNPIRTLRATGLPPRL
                     VALSAIRASALPVVSYLGPAAAALLTGSVVVETIFGLPGVGRYFVEGALNRDYTLVMA
                     TVVLVAVFVITFNLIVDILYAVIDPRVRYG"
     gene            17482..18603
                     /locus_tag="PK264_05875"
     CDS             17482..18603
                     /locus_tag="PK264_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD75454.1"
                     /translation="MADVAATPGRSLWQDAARRLARNRLAMGSLTLLAVVAIAVIIGP
                     FLTGHAHDRVYAEYVRVPAGIAAHPDHARIEGALERIAIRMRVKAELVRAHSGARAGE
                     VVAILEAPRPIDDRNLAYFERSGLFGAARVLERGEDGARVVVAAPIAYERFLLGTDTN
                     GRDLLSRTLIAGRVSLAIGLLASLVALVIGVAWGATAGYLGGRVDLVMMRIVDILYAL
                     PFMFFVILLVVFFGRSVALMFIAVGAIEWLDMARIVRGQTLTIRAQEYVQAAEALGAT
                     TRGILWRHIIPNAIGPVIVTVTMLVPRVILLESFLSFLGLGVQEPMTSWGVLIAEGAR
                     NIQGAAYMLAGPATALVATLFALNALGDGLRDALDPKER"
     gene            18603..20210
                     /locus_tag="PK264_05880"
     CDS             18603..20210
                     /locus_tag="PK264_05880"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRD75455.1"
                     /translation="MAPLLSVRDLAVTFETGDGAVAAVRGLSLDVERGETVAIVGESG
                     SGKSQTALAVMGLLAANGSAQGSAQFIVESGAAPVDLLALPARDLNRLRGRRLSMIFQ
                     EPMTSLDPLTRVGQQIGASLRHHQGMTRDAARDRAVSLLDRVRIRRPAERVDAYPHEL
                     SGGERQRVMIALAIANGPALLIADEPTTALDVTTQAGILDLIAELQRDLGMGLLFITH
                     DLTLVRRRAGRVVVMRGGKAVERGATADVLEMPKHDYTRTLLAAEPRRKDRSRAGVGP
                     VVVEARGIDVRFPVRRGWLGRSARKVHAVCGVDIDIRRGETLGVVGESGSGKSTLGRA
                     LLRLVPGSGLVRFEDRELAALTRAELRPLRRSMQLVFQDPFGSLSPRMTVGRIVTEGL
                     LVHEPQLSEVERDRRAAAALESVALDAALRNRYPHEFSGGQRQRIAIARAMILRPRLV
                     VLDEPTSALDRTVQATIVDLLVRLQREHGLAYLFISHDLAVVRAISDRIMVMRGGEVV
                     EEGAADDVVARPREAYTQALVKAAFEP"
     gene            20356..>21580
                     /locus_tag="PK264_05885"
     CDS             20356..>21580
                     /locus_tag="PK264_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRD75456.1"
                     /translation="MTSRAASDEAARARPAASRRDRSSERIREARAKLAQGTTIKPEF
                     EYELLSMFVRNELGALVTIPLLAMVFCLASMFWAPVGQAIFWLLAVIAAKVMLLECCR
                     RFVAAAREEIIIDVWRRRLILFELLSGVAWAGIALVGMTAKDPSSHVFIFASLIVVLA
                     IRMTFASSVMPILYVGTIPMTLAVVTRLVLLNHPFYWAMASMAIGVHVYFIFLAKGLN
                     STSLAMLEFRAEKDALIAELEEEKSISDEARRRAEGANVAKSRFLATMSHELRTPLNA
                     ILGFSEVMKLELLGKLDNATYKDYCGSIHDSGRHLLHLINEILDLSRIEAGRYELHEQ
                     PTRLADVAEDCYRLLKLRADGKGLQVILDLDQSLPQLWSDERAVRQVILNLMSNALKF
                     TPKGGKVTLRVAPSAD"
     assembly_gap    21581..21626
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21627..>21947
                     /locus_tag="PK264_05890"
     CDS             <21627..>21947
                     /locus_tag="PK264_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD75457.1"
                     /translation="KEEIPKVMQAFGQGSLAHETAEGGTGLGLPIVQNLIELHGGSFE
                     LHSELRKGTEAIACFPKGRVMQSMPPLQPLGQERHRQGDAPPAAPVRAGRRPRLRRAG
                     VEGQA"
BASE COUNT         3449 a         7494 c         7326 g         3632 t
ORIGIN      
        1 cccatggttc atgccgatgc gccgacctgg cgccgctgct gcgcaggagc ggctcaagtt
       61 caagtgggtg gacgacacgc tgagcgggct caggatcggc gtgccgttcg ctctcgtgaa
      121 ggaggtgccc gagtcgcgca gctggaacgg ccaccagtgg cagagcgagg atgggcagac
      181 gctcattacc accttcaagc atccgctatc caaatacacg ataaactcct acttccggac
      241 gatgctcggc ggccggccga actcgcgtat tgcccacatc gatctcacct ccgagagctt
      301 cgtcatcgag ggtgagtcgg tcggccccaa aggcccctac agctaccagg tgcgggccta
      361 ccaggtcggc gaccacatcg tcggaatgct cgcgcactac ccaccggccg tcgccgacga
      421 catggagcgg gtcgtcaacg ccaatgcctc ccgctttcag cgcaacaacg gctggcgcac
      481 catctacctc tccaactgcg gcagcaagca gccgggtgag gcgcgcatct tctacggcac
      541 gaaccgcgcg cagttgacgc cggcgaccgt tgccgctgac ggcgtcgaca tcgacggcct
      601 ttttgcgccg attccggcgg ggcagcttca tctcgggtgc gcccacgtct cggtgccgac
      661 cgacatcgaa gcgcgacgcg ccctcacccc ctcccctggg ctcgcgtcgg ccaatccgac
      721 agcccactac atcctgaagc gcttccgacc gatcacctcg agcgccgtcg gcgaccccgg
      781 ccggcgcatc gtgctcgtcg acgatactta cgccgagggc ggagagcggg cgctgatctt
      841 catccacggc tacaacgtcg gcttcggcga cgccgtccgc cgcatggcgc agatcgcaag
      901 cgatacgggc tacaagggca agatatacgt gttcagctgg ccctcgctcg gccagcgcac
      961 acgctatacc gctgacctcg acacggccga gcaatcggag gcctatctcg agagcttcat
     1021 ccgcctcatc ctcacggatc gcaacgtcca gacgctcgac gtcatcgccc acagcatggg
     1081 ctcgcagccc ttcctgcgtg tggtgcgcgg cgttgcccgc tccttcgacc agccgaaccg
     1141 gcgcggccga cgcatccgct tcggccaggt gatctttgcg gcccccgacg tcggcgcgga
     1201 ggtattcaag gaaaaggttc gcgagatcgt gccgctcgcc aagcgcgtga cgctctatgc
     1261 ctcgtcgacg gactgcgcca tgtacgcctc gcgcctcctc cggctcggtc gcggtcgcgc
     1321 gggcgatctc gagaatggca agccgctggt catcgatggt gtcgaaacga tcgatgcctc
     1381 cgatgtgcgg ccatccggcc tgctcgagcg cttcagcacg gttgtgaact ggggcaggct
     1441 caggaactac ttctcgtgca accatgccta ttttgccgac cggcaaaagc tgctcgacga
     1501 cctcaccgcg ctgctgcgca cggacgatgc gcctccaagc cgctcgtccg ccacgatgac
     1561 gcgcatcggc ccgagcgaca gccgttcggg ctattggcgt ctgccgccag agtgactccg
     1621 gatggccacc ctgggccact cccacgcgac agcccgcgct ctccgcaccg aaggcttgga
     1681 gaacggcaac cggccgctcc ccatgcaatt acccgacctc tgattgggag ccctgctgct
     1741 cttcgggaaa ttcaaatgta attggcccgg aacccgacga ggggcgcgcc aagatgccga
     1801 agcgccacta tcgaaaagcc tccaaaccat tactgtatga cttgcatttt cagatcgcga
     1861 aataaagccc gatcagcctt gtggccaatc gggctccaaa tctcgcggtc aagctcgaag
     1921 cgttagccga tcacactccg gatccatggc atcagggcaa ttcctcattg ccggatcttg
     1981 gccaggccgt catgcaagca ccaatctcgc taccagagtc atcgcctctc ggtcttgcca
     2041 cgtactggcg agccgtctga cgatgctgcc tctgccggag ccggtgctat ccaacgccaa
     2101 atataggcgc ttgctcggcg tgccatgtgt cgtgctgcct cgattgccgc gactgcgggg
     2161 ccagcccgcg tcaacgatcc aggaccacgt ctcgcctgaa ccctcgtcgc ctcgcatcga
     2221 cgctgcgata gtagcctttg gcgtactccg gcagacgact gctgacaact cggcctcatt
     2281 cgactgctgg ctgagatcgt tcggatatcg aatttttgag ggtgatagcc atccaggccc
     2341 gccgctcaaa tggcgacatg cgggacggct agatctggtc cggtggaagc atttttctag
     2401 acctccaatt ctggagtgcg gacggtctta ttctggcgcc gtcattgccg aattgtcaag
     2461 cgctccgtca aatgcaatct cgcaatgcaa aatgcgtcgc cgcgcatttc tgagccgggt
     2521 caactagccc gaactattca ccaccgcgcg gattgctcag ggcggggcag atcctgcaga
     2581 aggatggcga aaagcgtcgc tcttgcttgc acttgagcct tccgacgcac cgggcggccc
     2641 gccctgagcg acccgcaggg cggcgcgcaa ttgacgaatg gcgcctggct ggaagcaagc
     2701 ctccggaatg tctcgccgct cgaccgatgt ccccgcatcg ggctgcgcgg cactcctggc
     2761 cattcgtcaa ttttaccttc ctcgcgacca cgtgctggtg aatagttcgg gctagctgcc
     2821 aggcggccga ggcaatcgta ctgacgacca ttgggcatca acgaccatct gccgaccaac
     2881 gggcttgcct gcgcctcggg ctgcgctagt ctgagcggca tcagccaagg aggccacaca
     2941 tggaccaccg cacacttctc gatccgaccg gcgagcacga ggccgttgcg aagaagctcg
     3001 cggcccggcc gaggtcgctg gacggcgtga cggtcggcat cctcgatatc ggcaaggcgc
     3061 ggggcgacgt ctacctgaac cagctcgccc tgcggctcgg cgagcgcggc atcgccgtga
     3121 agcgctacgc caagccgacg ccgagccgtc tggcctcggg cgaaacgcgg gcgcgcatga
     3181 aaagcgaggt gcaggcggcg atcatcggcc tcgccgattg aggctcttgc acaacgtgca
     3241 gtttgcacga cggatcctat ctcgacggca ctggcattcc ggcactgatc gtggcgacga
     3301 cggcattcgt ccaagccgcg gatgtgcaat cgaaggctct cggctacacg ccaaccatca
     3361 cgtgggttcc gcaccccgtg caggatcgta ccgacctcga gctgcagcga ctcgccgatg
     3421 acacggtcga ccgcatcgtg cagggattga ccacggcgtg atccctgccc ggaccggagc
     3481 gcgtctgaag gccatggttc cggccgcgcc gggcgaggcg cgtcagcgcc ttgaccccgc
     3541 gccgagatcc aggccgagcc ctctcgcgat gctgccgacg acgcggcgcg gcgagaggcc
     3601 gacggacacg acgagcggcc gctcgtccgg ctgcggctcc tcgagggccg cgaactggct
     3661 ttccagcagc gttgccggca tgaagtgccc gcgccggcgc tgcaagcgct cgccgatcat
     3721 ctggcgcgtg ccgaacagat agacgagcgc cacgtccggg cggccgtcga gcagaacgtc
     3781 gcggtaccgc cgcttcagcg cggagcacga gacgatcgcc ggctcttcct tcgcgcgccg
     3841 ctcgtcgatg tacgcagcga tggccgcgag ccagggagcg cggtcgacat cgtcgagcgg
     3901 atggcccgag cgcatcttgg cgacgttggc cgccgggtgg aactcgtcgg catcgcggaa
     3961 cggccaaccg agcgcacggc cgagattctt acccgtggtg ctctttccag ccccggagac
     4021 gcccatcagc aggatgacgg tcggcggcag ctgtcgcgac gatcgcatgg gcatgctctc
     4081 acgtcgccga tttctgagcc gcaggccgtt tcggctcgac gtgcccgcca aagcccttgc
     4141 gcatcgccga gagcattttt tccgcgaacg tgtggccctg gcgagaacgg aagcgggcgt
     4201 agagcgccgc ggtcagcacc tccgcgggca ccgcctcctg caccgccgcc tcgacgcacc
     4261 agcgcccctc gcccgaatcc tccacatgcc ccgagtagct ctcgagcccc ggctcctctg
     4321 ctagcgccgc tgccgagaga tcgagcagcc acgacgtgat cacgctgccg cgccgccaca
     4381 cctctgcgat gtcggcgaca tcgagcgcaa gccggtgctc cgccggcagc gtcgggcctg
     4441 cggcgccgcg catgatgtcg aagccctccg caatcgcctg catcatgccg tactcgatgc
     4501 cgttgtggac catcttggcg aaatgtccgg ccccgttcgg tccggcgtgc acatagccat
     4561 gggcgggccg cggatcgcgc gcctcgcgat ttggcgtcgc gggaatggag ccggcgcccg
     4621 gggccagagt cgcaaagatc ggatcatggc gctcgaatac ctccttggcg ccgccgatca
     4681 tcaggcaata gccccgctcg aggccccaca caccgccgct tgtgccgaca tcgaggtagt
     4741 ggaggccctt ccccgacagc atggcggcga gacggatgtc gtccttccag ttcgtgttgc
     4801 cgccgtcgat gatcgtgtca cccgccgaga gcagggctgc gagctcgccg atggcggcct
     4861 ccgtcgcact ccccgccggc agcattaccc agacggtgcg cggggctgca agccgctgta
     4921 cgacgtcggc gaggccatct gccgcgaccg caccctcggc agcgagcacc gttccgagct
     4981 tcgggtcgcg gtcgtagacg actgccgtgt gcccgcctcg catgagccgg cggacgatat
     5041 tggcccccat ccggccgagg ccgatcatcc cgagctgcac gcttccctcc ctgttgctgc
     5101 cacgcgtcgc tgcggcctta tagcaggtgt gaccggtctg tgacgttgcc ccaatggcac
     5161 aacggctgcc tccagaaagt ccgccaattc gcttcgcaag cgggctgccg cccactcccg
     5221 ctcggcgctg aggccgcccc gaacctggcc cgttctgaca tcagagcaag ggggaggtcc
     5281 gatgggtctc ctcagggcgg agcgcgaggc ggcagcctcg ctcgcgcgag cgacgcgggc
     5341 gagttgccgt ggccgcgctc ggcttcaccc tgcaatcaac acttggaagc catcccgaca
     5401 ttcggcaaag tacggccact cacgagatgg acgacccgca atgcgccaac cactttccgg
     5461 catccgcgtc atcgacctga cgcgcatcct gtccggaccc ttctgcacga tgatgctcgg
     5521 cgacatgggt gccgacgtcc tgaagatcga gaccccagga ggcgaccccg tgcgcggaca
     5581 cggcgacatc gtcgaaggcc tctcctggta ctttgcagcg ttcaaccgca acaagcgctc
     5641 cgtcgtcatc gatctccgca agccagaggg caaggaggtg ctcgcccgcc tcctcgccaa
     5701 ggccgacgtg ctggtcgaga atttcaggcc cggcgtgctc accgaaatgg gcttcgatca
     5761 ggcgcgcctc gatgtgctca acccgcggct gattgtggcg agcataaatg gttacggctc
     5821 cgtcggcccc tacgtggacc gcccctcctt cgacttcatt gcccaggcca tgagcgggct
     5881 catggcgacc tccggcgagc ccgagggtgc gcccatgcgg acggcactcc ccatcaccga
     5941 tctcgtcgcc ggcctctatt gcgccttcgg catcgtcaac gcactccgcg cgcgcgatct
     6001 caacgggcgc ggccagcgtg tcgaggcggc gatggtgaac ggcatcgtct cgatgctcgc
     6061 ctacctcgct tccgagcacc tggcgacggg gcgcaatccg cagcgcacgg gcaacgatca
     6121 tccgatcgcg gcaccttatg gcctctatcg cgccaaggac ggcgagatcg ccatcgcgcc
     6181 gagcacgaac gaatcgctcg agaagctgat gcgcaccctc ggtctctccg agatgctgga
     6241 gcggccggag tacaggaccc cgcagctccg ccacgcccgc cgccacgact tccgcgcgcc
     6301 gatcaatgca gccctcgccg tcgagacaca ggacacatgg atcactcgtc tcaatgccgc
     6361 cggcgtcccc tgcggcaaga tcatgacgct gaaagaggtg ttcgccgatc cgcagatcaa
     6421 ggcgcagcag atggtgctcg agatcgatca tccgggtcac ggcgtcgtcc gcacggtggg
     6481 ctttccggtg aaactctccg gcacaccctg cgctgtccgc ttgccggtcc ctgagctcgg
     6541 cggccacacc gacgaggcgc tgcgcgagag cggcctcgct gatgccgaaa tcgccaagct
     6601 ccgcgccgcc aaagtcatcg tgtgagccga gccatggcaa gcaacctgca gatcaacgcc
     6661 gaccggctct ggtcgaccat catggcctcc gccgagatcg gcaagggtcc gcgcggcggc
     6721 ctctgccgcc tgacgctcag cgacgatgac cgcacggtcc gcgatctcgt cgctggctgg
     6781 gccaacgtcg ccggctggtc actcaccgtc gaccgactcg gcagcatgtt catcgcgcga
     6841 cggggccgcg agccgagcct gccgccggtc ctcatcggca gccatctcga cacgcaggcg
     6901 gcaggcggcc gcttcgacgg catcctcggc gtcatggcgg gcatcgaggt cctgcgaacc
     6961 ctggacgatc tcggcctcga gacacggcgc accatcgagg tcgtcaactg gacgaacgag
     7021 gagggcgccc gtttccagcc gccgatgctc tgctcgctcg ccttcgccgg caaggctgcg
     7081 gccgactggg ttctcgaccg gatcgacaag gagggccgcc gcttcggcga cgagctcgcg
     7141 cgcatcggct atgcggggag tgccgcggtc ggcggccgcg cgatcgacgc ctatttcgag
     7201 ctccacatag agcagggccc caagctcgat gccgccaagg tcccgctcgg cgtcgtggtc
     7261 ggcggatttc caaccgccgg catgcgcatc gagatcaggg gccgcacctc gcatgtcggg
     7321 ccgacgccga tggagcatcg ccgcaacgcc ctcgtcgccg ccgcccacgt cgcggtcgcc
     7381 gtcgacgaaa tcggctggcg ctatgcggcg ggcgagggca agacgaccgc tgcccgcctc
     7441 gacctctggc cgaacctccc cggcatcctc tcggagcgcg cgacactcta ctgcgatctc
     7501 cgccaccctg acgcgagcgc cctcgagcgc atgctggccg aggtcgcgac cgccattccg
     7561 gattgcgcgc gacgcggacg ctgcgagatc gagatcgccg agcgctgggg cttcggcggc
     7621 ttttcattcg atcgcgatct gattgcgctc atccgggcct ccgcagcgcg gcgcggcgtc
     7681 gcaactctcg acctcaagtc ggaggcgggt cacgacgcct accacatggc ctcgatctct
     7741 ccggcggcga tgatcttcac accctgcaag ggtggcatct cgcacaacga ggaggaggac
     7801 atgacgctgg cggaggccgt gcccggcgcc tccgttctcc tcgatgccgt tctcgagcgc
     7861 gccaaccgct gatcgactgt cctcccgcct cgccgtgcgt ccgcgatggg aggcggttcg
     7921 gggccagggc cagccccgat ggatggggct ccaccgtgtc cgtccccgcg aggggcaagt
     7981 ggcgcatgaa ccgaattact gagcgctgag aggttgtcat gcccgagtcg cacattccgc
     8041 cggacgacga tccgttcctg tggctcgagg aggtcgaggg cgagcgcgcg ctcgctttcg
     8101 cacgggccga gaccgcggcg accaaggccg cactcatgga cgaggcgttc gcgcgcgacc
     8161 gcgagatcgt ccgtgccatc atgaccgcgc cggacaagat ccccttcgtc agcgcgcgcg
     8221 gcgaccggat gtataacttc tggaccgacg ccgatcatgt gcgcggcctc tggcggcgga
     8281 caacgctcga gtcctatcgc tcgccgagcc ccgactggga aacgattctc gatatcgacg
     8341 tcctcaacaa gaccgagggt gcgaactggg tctgggcggg ctgccacgcg ctcgggccac
     8401 aattcgagag gggcctggtc tcgctctcga acggcggctc ggacgcggca gtcgtccgcg
     8461 agttcgatct cgaggcgaaa gcattcgtgc cggggggctt tgcgctcgcc gaggcgaagt
     8521 cgtcgctcga ctggctcgat cccgacacgg tgctcgtcgc aacgaacctc gcgccagcca
     8581 aacagacgac gtcgggttat ccgcgcgagg tccggcgctg gcggcgcggc gatccgttcg
     8641 agcgctcgga ggtgatctac gcgtgcgacg agacggacat gggcacgtgg gtgatggtcg
     8701 agcgcggtcc cgggccgacg ctcgttgcca tccaccgcgt gatcgacttc ttcagtcgtg
     8761 aggtctttct ctggcgcgag ggagcgccgc tcgtacggct cgatctgccg cgcgatgccg
     8821 agccgttgat ccgcgccgac cggctcgtca tcacgctgaa gagcgattgg gcggcgggtg
     8881 gctggagctt tgccaagggc tccgtcatcg cgatatcgct cgcccggttt ctcgcggggg
     8941 accgtgagtt cgccgtgctc ttcgagccga caccgcgctc gagcgtgcag gcgctgctct
     9001 ggatccgctc aggcatcgtg ctgacggtcc tcgacaacgt gcagagccgc atcgagattg
     9061 caagagaggc tggcgaccgc tggagggttg aaccgctggc cggactgccc gccggcacca
     9121 ccacatcgac catggccttc gaggccgatg acgcggcaag cacggaccgc ttcttcgtcg
     9181 tcgcgaacgg tttcctcacg cccgcaacac tttatcttgc cgaggcgggc acgctgccgg
     9241 tcaggctcaa ggaagcgtcg gcgcgtttcg atccgagcgg tctcaccgtt cgccagcacg
     9301 aggccgtctc gagcgacggc gagcgcattc cctatttcga ggttgcccgc gatgggctcg
     9361 cgcccgacgg ctcgagtgcc acactcatcc aagcctacgg cggattcgat ctgtcgatgc
     9421 tgccgggcta tcaggcgacc acgggccggc tctggctcga gcggggcggc gtgctcgtcg
     9481 tcgccaacct gcgcggcggc ggcgagttcg gtccgcgctg gcacgaggca gggcggcttg
     9541 cgggcaagaa gcagagccac gatgatcttg cagccgttgc cgccgacatc gcccggcgcg
     9601 gcgtatcgcg gccagagcgc atcgccgcgg tcggcggcag caatggtggc ctactcgtcg
     9661 gatcgatgct gacgcgttat cccgagcgct tcggcgcgat cgtgtgcggc gtccccctcc
     9721 tcgacatgcg ccgctatacg aaactgccac ccggcgcgag ttggatcgcg gaatacggtg
     9781 atccggacaa gcccgaggac tgggccttca tgcaggcatt ctcgccctat cagcagctcg
     9841 ccgccggccg gccctatccg ccgatcctcc tccacacctc gacgcgcgat gatcgcgtgc
     9901 atccgggcca cgcgcgcaag ttcacggcga ggttgcgtgc gctcggctac gagcccgtat
     9961 tctacgagaa cgaggacggc ggccacgcgg gagcagccga tcacgaggcg gtcgcaaccc
    10021 gggtggcgct cgagtatgcg ttcctgcgac gcgcgctgag gctggcagtg ccggcatgag
    10081 ggcgagcagc gaggggagtg ccggcttcgg cgtctacgtg cactggccgt tctgcgcctc
    10141 caagtgcccg tattgcgatt tcaactcgca tgtccgtgcg ggcgggatcg acgaggcgcg
    10201 gtatctgcgg gcctatctcc gcgagatcga ggcgagtgct gcggaaaccg gtccagcggg
    10261 cgcgcgcccc gtcgtcgaga gcatcttttt cggcggggga acgccctcgc tcatgtcgcc
    10321 gtcgacgacc ggcgcgatcc tcgaggcgat cggacgggca tggtctgttg cctccgctgc
    10381 cgaggtgacg atcgaggcca atccggggag cgtggatgcc tcgagatttg cgggctaccg
    10441 ggcggcgggt gtcaaccgcg tctcgctcgg gatccagtcg ctccgcgatg ccgacctgaa
    10501 gtcgctcggc cgccgccacg acgtcgccga ggcgcgccgc gccatcgccg tcgccgccgc
    10561 cacgttcgag cgcatgtcgt tcgacctcat ctacgcccgt gtcggacaga cggtggcggc
    10621 ctggcgcgag gagctcgccg aggcgcttac catcgcgggg agccacctct cgctctatca
    10681 gttgacgatc gagcccgaga cgccgtttgc cgcgctccat gccgcgggca agctgccggt
    10741 gccggacggc gagacgtcac tcaccctcta cgaggcgacg caggaagtga tggaacggca
    10801 cggcctcccc gcctacgagg tctcgaacca tgcggcgccc ggcgaagagt gccggcacaa
    10861 cctgctctat tggcgctacg gcacctacgc cgggatcggc ccaggcgcgc atggccggct
    10921 catgcgcgga ccgggtcgca ttgcaacggt cggcgagcgc aatccggagc gctggctcgc
    10981 gcgcgtcgtg gaggcggggc tcggctggac cgaggcgacg gcgctcacgc ccgaggagca
    11041 ggcggacgaa tgccttctga tgggcatgcg actgaccgaa gggatcgacc tcgcacgcct
    11101 cgccgatctc gcggggctcg cggccgatcg ccgccagctc gaagccctcg cgcgccatgg
    11161 cttaatcacg atagatagcg gacgtgacgc gcgcgcctcc ctcatccgcg cgacgtcggc
    11221 cggccggctc gtcctcaatc gcgtcattcc ggcgatctcg gagacgctcg cgccgattgc
    11281 cgctgccaca acccacaagg gaaccgcacg accatgacca atcccgtcct tgtcgaactc
    11341 acacgcggtg cactggtcga aagccagcat cggggtgcgg ttgccgttgc gcggccgaac
    11401 ggcgatctgg tcgtcgcaat gggagatgtg gcgcgccccg tctttccgcg ctcggccgtg
    11461 aagtcgctgc aggcgctgag cctcatcgag acgggtgctg ccgatcgcta tggctttggt
    11521 gcccacgaaa tagcgcttgc gtgcgcgtcc cactcgggga ccgaggcaca ctcctcgctt
    11581 gcggccgaca tgctggcccg cgccggtctg acggatgcgg ccctcggctg cggcgcgcac
    11641 atgccaggcc acgaggcgac cgcgcatggg aaaatcaaag ctggccgcgc gccgggcccc
    11701 ctcgacaaca actgttcggg caagcactcg ggcatgctgg caacggccgt ccacatgaag
    11761 gaggcggttg ccagctatct cgcgccggac catcctgtgc agcagcgcat cctgaagaac
    11821 atgcgcgagg tgacgggcgc cgcactcgcc gacgacgtcc gaggcatcga cggttgctcg
    11881 gtgccgacct gggcgatgtc gcttcagacg ctcgcgaccg ccttcgcgcg tctcggcagc
    11941 ggcgaggggc tcgggccaac gcgccgcgcg acggtggagc gcatcatggc gtcgtgctgg
    12001 gaggcgcccg agatggtggc gggacccggc cggctcgata cggcgatcct cggccgcctc
    12061 aagggccagg tgttctcgaa gaccggggcc gagggcgtct actgcgcggc gctcgtcgat
    12121 cgcgggctcg ggctcgcgct caaggtggag gatggcacac cgcgggcagc gcaggccgcg
    12181 ctcgccatga tcatagagcg gctttacccg agcgtcggca cgggccgctc ggccgcatca
    12241 cgaattggcg cggcaccgag gtcggcgcga cgcggggcgc gaacgagttc gagcaggcga
    12301 tcgaccgact cacggcgatg cggtaaggat ccgccagtat ccgtcatggc cgggcgcgtc
    12361 gcgggctgcg accacatgcc ccgcgcttga tcagggtcaa ggcccggcgt tgagatgggc
    12421 ggcactcgga tcactcgatc agcgctggcc gagagggcga gggcatggag acggagattg
    12481 ccgcgacaga ggcgccgtcg aacacggacg gcagcttcga gatctacctt gcctggcagg
    12541 acgtgacgct gacggtccgc ggcgagcagg ccgcaatcga cgtgctggtg caagccggtg
    12601 tgccgatcga gcccggctgc tccgtcggcg gctgcggcca gtgcgtgacg cgctatgtcg
    12661 agggcgacgt catccacaag gacgcctgcc tcaacgacga ggagcgcaag acgatgttct
    12721 gcccgtgcgt gtcgcgcgcc cggagccgca tcgtgctcgc gctgtagagc cggggaggat
    12781 cagccgaccg ttagcacgat cttgccgatg tggccgctcg attccatgcg ccggtgggcg
    12841 tcggcggcgt gggccagcgg gaaaatctga tccatgacga ccttgacgcg gccggcagcg
    12901 atcagcggcc agacccgctc ctcgagggcg cgcgcaaagc cggctttcac ggcgcgcgtt
    12961 cgcggacgaa gcgtggagcc ggtgagcgtc acgcgcttca tcatcagtcg ggcgacgttg
    13021 agctcgacga cggcgccctc gagcgtcgcg atctggacga tgcggccgtc ctctgcggcg
    13081 caaacaaggt tcttttccgt atagctgccg ccgaccatgt caagcgtgac gtcgacgccg
    13141 cggccgccgg tcacctcctt cacgacagcg acaaaatcct cagtgcggta gttgacggcg
    13201 cgatcggcgc cgagcgcgcg gcaggcggcg cacttctctt cactgccggc cgtcgcgatg
    13261 acgttggcgc cgaaggcctt ggcgagctgg atcgcggtgg tgccgatgcc gctcgtgccg
    13321 ccgtgcacca tgaaccactc accagccttg aggccgccgc gctcgaagac gttgttccag
    13381 acggtgaagt gcgtttcggg gagcgccgcc gcctcgatca tgccgaggcc agcagggata
    13441 ggcagcgccg acgtctcctc ggcaatgcag tattgcgcgt aaccgccgcc gttgacgagg
    13501 gcggagacct tgtcggcaac gcgccagcgc gtgcactcgg ggccgaccgc caccacctcg
    13561 cccgcgatct cgaggcctgg cagcggcgaa tgccccttgg gtggcggata ggcgccaagg
    13621 cgctgctgga catccggccg gttgacacct gccgctgcga ccttgacgag gagttgcccc
    13681 ggtcccggct cgggaacggc catctccctc ggcacgagga catcgggtcc acccttgccg
    13741 tcgatgacga tggcggtcat gcggtcggga agcgaggtca tggacgagcg gctccggaat
    13801 caatcgcgcg tgtgacggtc gaggaacggg atgacctgct tgtggaactc agccggcgcc
    13861 tcgccgtgca gcatatgcga gacgcccggc atgacgacga ggtcggagcc tgggatggcc
    13921 ttgtggatcg cctcggcggc agcgaccggc gtcgagggat cgagcgcgcc gacgatgatg
    13981 cgggtcggca ggcggatctc gccaaggcgt cccgtgatgt cgagttctcg gatggcgccg
    14041 ccccagccgc agtaaccttc cgccggtgtc gcctcgatca tgcgcgtgaa gcgggcgacg
    14101 agcggcttgt tcgacttgag cgcctcgggc gagacccagc gctgcaaggc gccctgcacc
    14161 tgcgatttca tgccggaggc gcgcacggcc gcgatccgct cgtcccacag cgcccgggct
    14221 tccggcggca cgcggctcga ggtcgaaacg agcaggaggc aggcaaagcg ctcgggatgg
    14281 agaagcccga ggtactggcc gaccatgccg cccatggaga ggccaaggaa gcgcgccttc
    14341 ctggcgccga catgatccat cagcgcgacg acatccttgg cgagcgtctc gaaggcgtag
    14401 ggagccggcg aggcctcggt gacgccatgg ccgcgcgcat ccatgcggat cacgcggtac
    14461 ttgtgcgtga gcgccgccgt cagctcgtcc caggtggtga gattggtggc gagcgggtga
    14521 tggagcacga cgacaggccc gcgctccggt ccggcgacct cgaagttcat acggatgccg
    14581 ttgatctgcg ccttcatggt gcactcctgt gatcgccgtt ggccgacacc gtaacgtgcg
    14641 tcacgggcgc ggacaacgcg cgaaaaaaga atgcgtggac ggctgctgcc gcgcctcagg
    14701 tcttgacgat cagacggttc gtcaggccct cggcgagacg ctggagcgcg gcacgcaagc
    14761 catcatcggc gactgtcgac agcggcagga tatcgatggg cttttgcgga cgcacgggag
    14821 cggcacgctg aggaggagac gtcttcgcgt agcgctgctc gatgcggacc tcggcgatgg
    14881 ccgagtagcc gaaatagcgg tttacgcggt cgatgatctg ggcgcgctga tattcgacgt
    14941 cgagcgcgcg ggccggatcg acggcgatga agagcgtagc gccgggacga ccagtcgcct
    15001 cgtcgtcgct gcccgcgatc ttggcgggaa ggcgcggcca gcgcagccgg tccggtgtcg
    15061 tgtagcccgc gagtgtcggg ccgacgatcg cgcaccaatc ggtgattagg gtcgcggccg
    15121 agaaaccaaa cttctcgaac gacggccgtg tcaaacgagg cacatagctg ccgacggcgc
    15181 gtgggccggc agccttggcc gcgacctgac gcacggtggc gctcctcgac gctcgatcag
    15241 cttgcgacac gatcgacctc tccgtgctgc ggcaatccgg ctggggtttc ccgacagtgc
    15301 ctctcgactg agccaccgga cggacgggcg aacctgctat cctcaatgcg atgattcggg
    15361 tgacgtcgcc cactccccac ctcagggagg gacatatggc tgtggatgca tcgatcggcg
    15421 cggcagagaa gccgcgaccg agccgccggc aagcgcccga gagaggcgca gcgagcgtcg
    15481 gcaggcgcct gctcgcctgg tacgacctcg agcgacgcga gctgccctgg cgctacccgc
    15541 cggggcaaac ggccgatcca tatagggtct ggctatccga ggtgatgctg cagcagacga
    15601 cggtaaaggc ggttgccccc tatttcgagg ccttcacgcg ccgctggccg acggtcggtg
    15661 cgctcgccgc agcacacctc gatgacgtgc tgacggcttg ggcggggctt ggctactact
    15721 cgcgtgcgcg caatctgcat gcttgcgcca aggtcgtgac tgcagaccat ggcggcgtat
    15781 tcccgtcgcg ggaggccgcg ctcgcagagc tccccgggat cggcccctac acggcggcgg
    15841 ccatcgcctc gatcgctttc ggacggcgcg caaccccggt cgacggcaac gtcgagcggg
    15901 tggtggcacg gctgttcgca atcgaacggc cgctgcctgc tgcaaagacg gagctcgcgg
    15961 cccttgccag cacgctcacg ccgcagcgcc gggcgggcga tttcgcgcag gccatgatgg
    16021 accttggcgc caccgtttgc acgccgcggc ggccgagttg cctgatgtgt cctctggcca
    16081 ccggctgcaa ggcgcgagag gcagggttgg cagagcggct tcccgcccgc gccgccaagg
    16141 ccgagcggcc gacacggttc gggatcgcct tcctggcgct ccgggaggat gggcgcctat
    16201 tgctccgcca gcgcgacggc aaagggctgc tcgcctcgat gatcgaggta ccttcgacat
    16261 cctgggtcga tacctggccg gccgaggatg aggcgctgca ggcggcgccg gtcaatgcga
    16321 gctggtggcg cgtcccgggc gtcgtaacac acgtgttcac gcacttccgg ctcgaattgc
    16381 ttgtctaccg ggcgatcgtt ccagtcgatg cgcaactcac gttctgggcg aatgcgccgc
    16441 gctgccgctg ggtctcacgg cgcgcgctcg gcgagcaggc gctgccgagc gtgatgacca
    16501 agctcatcgc ccatgcgatg aaggacgggt gagccggcga ttgcggccac atccggtccg
    16561 ggggagacat gcttgcctat gtgctccgca ggctgctgac agcgatccca acgctgttca
    16621 tcgtcgtgac catctcgttc tttctgatcc gcgtcgcccc tggcgggccc ttcgacctcg
    16681 agcagccgct cgaggccaag gtgatggaga atctgcgccg catctatcag ctggaccggc
    16741 cgctgatcga gcagtacggg cgctatctcg cggcactgct ccgaggcgat ttcgggccct
    16801 cgttctacta ccgcgacttc accatcgccg agctgcttcg tcgcgggctc cccgtgtcga
    16861 tgctgctcgg cgcgctcgcc ctcgccctcg cgctggttat cgggacggca ctcggcgcgc
    16921 tcgcagcctt ccgccagaac gggctcgccg actacgccgt gatgagcctt gcaacgctcg
    16981 ggctcacggt gccgggcttc gtcgtggcgc ccctcctcca gatcgtcttc gggctgatgc
    17041 tcgcctggct gccggtcggg ggctggaacg acggcgcggt gcgcaatctc gtcctgccgg
    17101 tgctcgtgct ggcgctgccg caggtcgcca taattgcgcg cctcacccgc gcgacgatga
    17161 tcgagacgct gcgctcaaat ccgatccgca ctctgagggc gaccggcctg ccgccgcggc
    17221 tggtcgcact ctccgcgatc cgcgcctccg cgcttcccgt ggtctcgtat ctcggtccgg
    17281 cggcggcggc gctgctgacg ggctcggtcg tggtcgagac catcttcggg ctgccgggcg
    17341 tcgggcggta cttcgtcgag ggggcgctca accgcgacta cacccttgtc atggcgacgg
    17401 tggtgctggt tgccgtgttt gtcatcacct tcaatctgat cgtcgatatc ctctatgccg
    17461 tgatcgaccc gcgggtgcgc tatggctgac gtcgcggcaa ctcccgggcg ctcgctctgg
    17521 caggatgcgg cccgccggct cgcgcgcaat cgccttgcca tggggagcct cacgcttctt
    17581 gcagtcgtcg cgatcgccgt gatcatcggc ccgttcctga cagggcatgc gcacgaccgc
    17641 gtctatgccg agtacgtgcg cgtgccagcc gggattgctg cacatccaga ccacgcgcgg
    17701 atcgagggcg cgctggagcg catcgcgatt cgcatgcggg tgaaggccga gctggtgcgg
    17761 gctcactcgg gcgcgcgggc gggcgaggtc gttgccatac tcgaggcgcc gcggccgatc
    17821 gacgatcgca acctcgccta cttcgagcgc tccgggcttt tcggggcggc ccgcgtcctc
    17881 gagcgcggcg aggacggtgc ccgcgtggta gtggcggccc cgattgccta cgagcgcttc
    17941 ctgctcggca ccgacacgaa cggccgcgat cttctctcgc gaacgctgat cgcggggcgc
    18001 gtgtcactgg cgatcggcct cctcgcgtcg ctcgttgccc tcgtgatcgg tgtcgcctgg
    18061 ggggcgacgg ccggctatct cggcggccgc gtcgatctcg tcatgatgcg gatcgtcgac
    18121 atcctctatg cgctcccgtt catgttcttc gtcatcctgc tcgtcgtgtt cttcgggagg
    18181 agcgtcgccc tgatgttcat cgcggtcggc gccatcgagt ggctcgacat ggcgcgcatc
    18241 gtgcgcggtc aaacgttgac gatccgcgcg caggagtatg tgcaggccgc cgaggcgctc
    18301 ggagcgacga cccgcggcat cctctggcgt cacatcattc cgaacgcaat cgggccggtg
    18361 atcgtcacgg tgacgatgct cgtcccacgc gtgatcctgc tcgagagctt cctctccttc
    18421 cttggcctcg gcgttcagga gccgatgacg agctggggcg tgctgatcgc ggaaggggcg
    18481 cgcaacattc agggcgcggc atacatgctc gccggccccg cgacggcgct cgtcgccacg
    18541 ctgttcgcgc tcaatgcgct cggtgacggc ctgcgcgatg cgctcgatcc gaaggagcgc
    18601 tgatggctcc gctgctctcg gtgcgcgatc tcgccgtcac gttcgaaacg ggcgacggcg
    18661 ctgttgccgc agtgcgcggc ctctcgctcg atgttgagcg cggggagacg gtcgcgatcg
    18721 tcggcgaatc gggctcgggg aagagccaga cggcgctcgc ggtcatgggt ctgcttgccg
    18781 cgaacgggag cgcgcagggc tcggcgcagt tcatcgtgga aagtggcgcc gcgccggtcg
    18841 atctgctcgc gctgccggct cgcgatctca accgactccg cggccggcgc ctctcgatga
    18901 tctttcagga gccgatgacc tcgctcgatc cgctgacgcg tgtcggccag cagatcggag
    18961 cctcactccg tcatcatcag ggaatgacgc gtgacgccgc gcgcgaccgc gcggtctcgc
    19021 tgctggatcg cgtgcgcatc cgccgccccg ccgagcgcgt cgatgcctat ccgcacgagc
    19081 tttcgggcgg cgagcgccag cgcgtcatga tcgcgctcgc gatcgccaac gggccggcgc
    19141 tcctcatcgc tgacgagccg acgaccgcgc tcgacgtgac gacgcaggcc gggatcctcg
    19201 acctcatcgc cgagttgcag cgcgatctcg gcatggggct cctgttcatc acgcacgacc
    19261 tcacgctggt acgccggcgc gcggggcggg tcgtggtgat gcggggcggc aaagccgtcg
    19321 agcgcggggc aacggcggac gtcctggaga tgccgaagca cgactatacg cggacgctgc
    19381 tggcggccga gccgcgacgg aaggatcgct cgcgcgcggg ggttggtccg gtcgtcgtcg
    19441 aggcgcgggg tatcgatgtc cgctttcccg tccgtcgcgg ctggctgggt cggagtgcac
    19501 gcaaggtgca tgcggtctgc ggcgtggata ttgacatcag gcgcggcgag acgctcggcg
    19561 tcgtcggaga gtcaggttcg gggaagtcga cgctcgggcg cgcgctgctg cggctcgttc
    19621 ccgggtcagg cttggtccgc ttcgaggatc gcgagcttgc cgcgctcacc cgggcggagc
    19681 tccgcccgct gcggcggtcc atgcagctcg tcttccagga cccgttcggc tcgctctcac
    19741 cgcgcatgac cgtcgggcgc atcgtcaccg aggggctgct cgtgcacgag ccccagctca
    19801 gcgaggtgga gcgggaccgg cgggcggcag cggcgctcga gagtgtcgcg ctcgatgccg
    19861 ccttacgcaa ccgctacccg cacgaattct ccggtggcca gcgtcagcgc atcgccatcg
    19921 cgcgggcgat gatcctgcgg ccgcgcctcg ttgtgctcga cgagccgacg agcgcgctcg
    19981 accgcacggt tcaggcaacc attgtcgatc tcctggtgcg cctgcaacgc gagcacggcc
    20041 tggcctacct gttcatcagc catgacctcg ccgtggtgcg ggcgatttcc gaccggatca
    20101 tggtgatgcg cgggggcgag gtggtggagg agggcgcggc ggacgacgtc gtcgcccgtc
    20161 cgcgtgaggc ctacacgcag gccctcgtga aggccgcgtt cgagccctaa tcggttcacg
    20221 cgaccagtac aattgcgggc agccgttcaa ccgtccgtta gcgaatattc gtgattagta
    20281 agctgcacgg catggagctc gttggcttgc cgacacaacg cccgaactct ttcgggtccg
    20341 aagaggatag cgcatttgac ctcacgagcg gcgagcgacg aggcagcgcg ggcgcgaccc
    20401 gcggcgagcc gccgcgaccg ttcgagcgaa cgcatccgcg aggcgcgggc gaagcttgcc
    20461 cagggcacga cgatcaagcc cgagttcgag tacgagctcc tttcgatgtt cgtacgcaat
    20521 gagctcggtg cactagtcac gatcccgctg ctcgcgatgg tgttctgcct cgcctccatg
    20581 ttctgggcgc cggtcggcca ggctattttc tggctgctcg cggtgatcgc ggccaaggtg
    20641 atgctgctcg agtgctgccg gcggttcgtc gcggctgccc gggaagagat catcatcgat
    20701 gtctggcggc gccgcctcat cctgttcgag ctgctgtcag gtgtcgcctg ggccgggatc
    20761 gcccttgtcg gcatgaccgc aaaggatccg tcgagccacg tcttcatctt tgcctcgctg
    20821 atcgtcgtgc tggcgatccg catgacgttc gcctcgagcg tgatgccgat cctctatgtc
    20881 ggcacgatcc cgatgacgct cgctgtcgtg acgcgactcg tgctgctcaa tcacccgttc
    20941 tattgggcca tggcgtcgat ggcgattggt gtgcatgtct atttcatctt cctcgcgaaa
    21001 gggctcaact cgacctcgct cgccatgctc gagttccgcg ccgaaaagga tgccctgatt
    21061 gccgagctcg aggaggagaa gtcgatctcc gacgaggcgc gccggcgcgc agaaggcgcg
    21121 aacgtcgcca agtcgcgctt cctcgcaacc atgagccacg agctgcgcac gccgctcaac
    21181 gcgatcctgg gcttctccga ggtcatgaag ctcgaactgc tcgggaagct cgacaacgca
    21241 acctacaagg actactgcgg cagcatccac gacagcggcc gccaccttct ccatctcatc
    21301 aacgagatcc tcgatctctc gcgcatcgag gctggccgtt acgagctgca cgaacagccg
    21361 acgcggctcg ccgacgtcgc cgaagattgc taccggctgc tgaaactccg cgccgacggg
    21421 aagggcctgc aggtcatcct cgatctggac cagagcctgc cgcagctgtg gtcggacgag
    21481 cgcgccgtcc ggcaggtgat cctgaacctg atgtcgaatg cgctcaagtt cacgccgaaa
    21541 ggtggcaagg tcacgctgcg cgtcgcgccc tctgccgatg nnnnnnnnnn nnnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnngaag gaggagattc cgaaggtcat gcaggccttc
    21661 gggcagggct cactcgcgca cgagacggcc gagggcggaa cgggtctcgg cctgccgatc
    21721 gtgcagaacc tgatcgagct gcacggcggc agcttcgagc tccactctga gcttcgcaag
    21781 ggcacggagg cgatcgcctg cttcccgaaa gggcgcgtca tgcagtcgat gccgccgcta
    21841 cagccgctcg gccaggagcg gcaccgccag ggcgatgctc cgccggcagc tccggtgcga
    21901 gcgggccgga ggccacggtt gcgccgcgcg ggcgtcgagg gccaggc
//
LOCUS       DAORNH010000036        21667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1873_length_21667_cov_2.825097, whole genome shotgun sequence.
ACCESSION   DAORNH010000036 DAORNH010000000
VERSION     DAORNH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21667
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1873_length_21667_cov_2.825097"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK264_05895"
     CDS             <1..969
                     /locus_tag="PK264_05895"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD75458.1"
                     /translation="AYLQRVGQMESTTTRLKGILEDVIKSNPGKTRDQIIALFEERKK
                     AFVMELQKQAIASARAGVVPTKRKEAENEIIEERLKLQEAKRLGVVVTDDDVDRVIKQ
                     IADKNKMTVPKFAEHMKSMGADIAVMRSRFRATLAWREVIRRRFGPQIAISNKEIDAH
                     LAGAGAGAAGADSILLHVHRFILPVSGANQAALAQRFAEAQRMQKAFAGCKSSPGLVK
                     AAAGARFEDLGFKPTGALPVKEETRTFLLTARDGEMVPPSITAAGIELFAVCARRVTS
                     GTDAKREKAQEELMMREFETYSKRHLRFLLHEAQIHRQTPVAPGKS"
     gene            966..>1202
                     /gene="pdxA"
                     /locus_tag="PK264_05900"
     CDS             966..>1202
                     /gene="pdxA"
                     /locus_tag="PK264_05900"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065985.1"
                     /note="catalyzes oxidation of
                     4-(phosphohydroxy)-L-threonine into
                     2-amino-3-oxo-4-(phosphohydroxy)butyric acid which
                     decarboxylates to form
                     1-amino-3-(phosphohydroxy)propan-2-one
                     (3-amino-2-oxopropyl phosphate); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase"
                     /protein_id="HRD75459.1"
                     /translation="MSRSRRALALTLGDPSGLGPDITIAAWQQRHTHGIAPFVAIGAR
                     QALVERAGALGLAIPIEAVTDPGAALACFDRSLPV"
     assembly_gap    1203..1212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1213..2009
                     /gene="pdxA"
                     /locus_tag="PK264_05905"
     CDS             <1213..2009
                     /gene="pdxA"
                     /locus_tag="PK264_05905"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRD75460.1"
                     /translation="IPTDVRVEAGRPTSTNAEAIVRSIERAVAFAEAGEAAAVVTCPI
                     AKSVLQSAGFKHPGHTEFLAHLAETRRRGTTIRPVMMLAGPDLRVVPLTIHIPLADVP
                     RAISRPLIIETTRITADALRRDFGLASPRIAITGLNPHAGESGTLGREEIETIGPAIG
                     ELKAGGLHVTGPHSADALFHAAARATYDAVIAMYHDQALIPLKTLAFDDGVNVTLGLP
                     FVRTSPDHGTAFDIAGTGAARPESLIAALKLASEIATRRAAAPTRG"
     gene            2016..2564
                     /locus_tag="PK264_05910"
     CDS             2016..2564
                     /locus_tag="PK264_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRD75461.1"
                     /translation="MPAPIVRAVSRNAIYGFSKPEAESIRLITGLGVEDDIHAGETVK
                     HRSRVARNPSAPNLRQVHLIHAELFDELRAKGFLKPRPGALGENVTTGGIDLLGLSAG
                     TRLRLGREAIIEVTGLRNPCSQLDAFEAGLMGAVLEKSRDGRLVRKAGIMAIVISGGG
                     VRAGDAIEVVALPAVHAPLEPV"
     gene            2567..3484
                     /gene="rsmA"
                     /locus_tag="PK264_05915"
     CDS             2567..3484
                     /gene="rsmA"
                     /locus_tag="PK264_05915"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRD75462.1"
                     /translation="MSDESKPDIATAAEASRAGASPDGLPPLRDVISALDLRASKALG
                     QNFILDLNLTRRIARTAGTLSGRTVVEIGPGPGGLTRALLLEGAERVIVIERDDRCRP
                     ALEAIGARYPGRLTIHMGDALAIDWLRLVAGAPDKPIIAANLPYNIASRLLVGWLESE
                     PWPPWWSRMILMFQREVADRLVAVPGTKAYGRLSVISQWRARVKLALTLGPEAFTPPP
                     KIASAVVDFSPISEPAFGCSATALGLVTGAAFGQRRKMLRQSLKTLVPDPAAILDAAG
                     IAGELRGEQLSVHDFARLTHAAERAGLQR"
     gene            complement(3488..4390)
                     /locus_tag="PK264_05920"
     CDS             complement(3488..4390)
                     /locus_tag="PK264_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD75463.1"
                     /translation="MLNDTRELPVWRSMLFVPVNVPKFVERAHERGADAIILDLEDSI
                     PPAEKANARALVREAAPRVARNGADVVVRINRPLDLAVRDIEAAVMPGVAALMLPKVE
                     SASHVQLLAELVASVEAARGVPVGQTKFLALIETPSAFEQAAEIARADPRLVALSLGS
                     EDFALEIGGAADPEVLTYPKQRVAIAAIAAGLMPMGVIGSVADYSDREGYKAAVLRSR
                     RFGFRGSSCIHPALVPILNDGFTPSAVDLEHARRVIAADDKARAEGRGSAELDGRMID
                     VPIVVKACRLIAMAEKIAARAKRG"
     gene            complement(4798..5631)
                     /locus_tag="PK264_05925"
     CDS             complement(4798..5631)
                     /locus_tag="PK264_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD75464.1"
                     /translation="MRMVTLPGGEKVPALGLGTWHMGERRSDRAAEAKALRAGLDVGL
                     TLIDTAEMYGEGGAEEVVAEAVAGRRDQVFIVSKVYPHNASRAGAIAACERSLARLKT
                     DRIDLYLLHWRGSYPLAETVAGFEALKASGKIRHWGVSNFDVDDMAELAGVAKPGAWA
                     ANQVLYHLGSRGIDFDLIPAMAKASVAIMAYSPLGQGAILRNPALAKIAARHTVSPAA
                     VAVAWGLRHPHVISIPKASKLAHVAENAKAADLVLTAEDLAELDRAFPPPKKASPLGI
                     L"
     gene            6011..8239
                     /locus_tag="PK264_05930"
     CDS             6011..8239
                     /locus_tag="PK264_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD75465.1"
                     /translation="MCLTSRTRAIGVLLLVPLAMAPIAARAQTTLPGVTVSTPSPVTP
                     SKGATSPTPPSSADATSGKSPAKAKTAAPAPAVTPSAPPPALPLPGTLIVADDSFVPV
                     TVILDGALAPNPSASLADLLSTTPGVAASTFAPGASRPIIRGLENNRVRIQDNGVASH
                     DVSALSEDHGVPIDPLVGGRVEVIRGPATLRYGSQSIGGVVSAENNRIPTIIPPSGFS
                     LETRGGWTSVNSGRDGGLIVDVGSGNVAIHADAFSRDAGDYRTPHGVQPNSSLHADGG
                     SLGGSLIFDKGYFGVAYTRYASLYRIPGVEAAERALRIDLDQWRLTSKGEWRVESGAV
                     DRIRFWFGRTEYRHDEISIDPGTGEREIGSTFRNTELEGRIEVQHRPLLMAFGRLTGA
                     VGLQLGDRRLSAAGEGGELLAPNRTDTAALFIFEELQPTDRLRIQAAARMERARVRGT
                     AALFPDTFLPPPDEPDLEGRNRTFKPRSVSLGILHELPLGIVARVTGQLVERAPDAAE
                     LFSKGPHEATQTFEIGNPNLAIERARTLEIGFRRARGDFRFDATAFHTEFDGFIFKRL
                     TGVTCDDDFASCGAGSELEQLVFGQRDATFQGVEIAAQWDVIPIWRGVLGIDGQYDFV
                     RARFEDGSAVPRIPPHRAGAGVYYKDGRWYARVGFLHAFRQDEIALGETSTSSYSLLN
                     AELSYTIRPSATGPGAREIVIGLRGDNLLDDDVRNHVSFRKDEVLQPGRSIRLFGTLK
                     FN"
     gene            8347..9522
                     /locus_tag="PK264_05935"
     CDS             8347..9522
                     /locus_tag="PK264_05935"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004690454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HRD75466.1"
                     /translation="MTTSPRPAKRPANACFSSGPCAKRPGWTPEALGKAFLGRSHRAK
                     EGKARLKLAIDKTASVLGLPQGYRTAIVPGSDTGAYEMAMWTMLGARGVDALAWESFG
                     AGWVTDITKQLKLADVRVLEAPYGTLPDLAKVDFSRDVLFTSNGTTSGVRVPNYDWIP
                     ADRQGLTFADATSAAFAQPIDWTKIDVATFSWQKVLGGEAAHGMLVLSPRAVERLETW
                     KPAWPLPKVFRLTKSGKLEAGVFAGETINTPSMLCVEDYLDALAWAEGVGGAKGLNAR
                     SDRNAKALFDWVERTPWIANLAVDPATRSNTSVCLKIIDPAIAALPAEKQAAFAKDLA
                     ALLEKEGAALDIGSYRDAPPGLRIWTGATVETADIEALLPWLDWGFATLKAALAKAA"
     gene            9667..11469
                     /locus_tag="PK264_05940"
     CDS             9667..11469
                     /locus_tag="PK264_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRD75467.1"
                     /translation="MAPKDEISTIDESGSEKKPRNLRPLAGLAPQLLRHRGALAGAAA
                     ALVVSALAMLAMPVAVRHMIDQGFSGRDGGSINRYFLILIGIGLVLAAASSTRAFLVN
                     WLGERVVADVRRDVFSHLTTLSPAALETARSGEILSRLTADTTLVKSAAGSALSQAVR
                     NLIMLIGAVAMMFVTSPTLTLVMLAAIPAIVLPLMGIGRIVQRLSRRAQDTLAEASAY
                     AGENLSAVRTMQAYTHERTVVARFTSAVEAAFAASVDRLKARAGLAALTIALIVGSIV
                     GVLWYGATMVVANEMTGGRLGQFVLYALFAGGALAELAEVWGEVSQAAGAAERLAELL
                     ALAPGITAPRDPLPMPNPARGAIELDRVSFSYPSRPEVKALDHIMLRIEPGETVAIVG
                     SSGAGKSTIFNLLLRFYDPTAGEVRVDGVPTQRTDPKALRERFSIVPQEPALFADTVA
                     ENIRYGAPGASPADIERAAELAGALTFIRALPKGFETVLGERGTTLSGGQRQRVAIAR
                     AILRDAPILLLDEATSALDAESEASVQQALDAVMANRTTIVIAHRLATVLKAKRIIVM
                     DSGRIVEEGTHAALVARGGLYSRLAALQFRAAAE"
     gene            complement(11487..12629)
                     /gene="purM"
                     /locus_tag="PK264_05945"
     CDS             complement(11487..12629)
                     /gene="purM"
                     /locus_tag="PK264_05945"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRD75468.1"
                     /translation="MTKTTKASGAGSGKPPLTYHDSGVDIDAGNALIQAIKPLVKATR
                     RPGADADLGGFGGLFDLKRAGFRDPILVATNDGVGTKLKIAIDTGRHATIGIDLVAMC
                     VNDLVVQGAEPLFFLDYFATGGLDVEVARTVIAGIAEGCRESGCALVGGETAEMPGMY
                     KAGDYDLAGFAVGAVERGEVLPRHDIAVGDVLIGLASSGVHSNGYSLVRRIVEREGLA
                     WAAPAPFAASGDLAAALLAPTRLYVKPVLSAIRATGGSGPHGAVKGLCHITGGGLAEN
                     LPRVMPKGLAAHVRLDTVSPPPVFGWLAKAAGIGDTEMLRTFNCGIGMVVVADPARVA
                     DVTSALSAAGERPVAIGEVEPGRGVKSQAKGQGEAEAVRFSGRLRS"
     gene            12874..13983
                     /locus_tag="PK264_05950"
     CDS             12874..13983
                     /locus_tag="PK264_05950"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HRD75469.1"
                     /translation="MAAQLQGSAFAAPADKVFVVGSYPVEASAGDATTAKEKALAEGQ
                     QAAFRSLLKRLVPVTSYNRLPRLKNVKAAELISGYAVRSERNSDTDYVATLDFTFQAE
                     AVRNLLRREGVPFLDEQAPQTIVVPVWRAAKAAGPAASNAALADAKAAPVWEDVWKGL
                     DLENSLTPLKLETTKADLKPDTVKGLVDGQLDLIRSIAASHRAERVMLAVAEPDISGK
                     KLIVTIVGQDAVGAFHLQKAHKLEGSELAYTAELAAVISLGIMEGRWKAVRLRGGAAA
                     SLPRPGGPALTGGPAGADTVIHVEFRGMGEWQHISRALSATPGVENLDVLGLSARGAR
                     ITLAYPGGPEALAQVLRNQRIILHNTTQGWKLRAE"
     gene            14029..15087
                     /locus_tag="PK264_05955"
     CDS             14029..15087
                     /locus_tag="PK264_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD75470.1"
                     /translation="MKGVVFPGNRQTEIRDFPDPTPGEEEVVLEIKASGMCGSDLHIY
                     RSAGGGAAQARALGLGGEGGEVIAGHEPCGVVVARGKNVGDRQARLGARVMQHHYHGC
                     GTCPDCVDGWSQMCGAPGKTVYGITGHGGHAKYMKCPAHTLIALPDALSFETGAAISC
                     GTGTAYGALRRLDMNGGKTLAVFGQGPVGTSATMLGKAMGVRVIALDVSDERLTLAKA
                     CGADATVNPTKVDAVEALKSLTGGHGVDYAVECSSAETARAAIRGARSWGKVCFVGEG
                     GTVSIDVSPDMLRKQLTLLGSWTFNKQRQDECARFIAANKVPIETLISDRYSLGEAEA
                     AYKKFDKQTMGKGMILPA"
     assembly_gap    15204..15253
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15254..16158)
                     /locus_tag="PK264_05960"
     CDS             complement(<15254..16158)
                     /locus_tag="PK264_05960"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRD75471.1"
                     /translation="MLLKWSVAVFVGLAACDEGVPTAAAQSLRPATKVVRDTPVRPEG
                     PLTIVVSLGTQKVTVYAAGRHWSEAAISSGRDGYETPRGVYSVLEKRRYHESNIYSGA
                     PMPFMQRLTWSGIALHEGHLPGYRASHGCIRLPSSFAAALWEATKVGDRVVVVDAPVA
                     PVDIRHPHLFVARPLPADEQEALRRAEADTRDPDTKGPSPDRHLSIFVSRKDKTVYVR
                     RGFHPIFDAPVTIEAPEQPIGTHVFSAAELTDGGDGMRWTVVTVSSGRETRGNERVAA
                     LKRRGQTTDADAAPPTADATAAGALS"
     gene            16372..17007
                     /locus_tag="PK264_05965"
     CDS             16372..17007
                     /locus_tag="PK264_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD75472.1"
                     /translation="MSPPEYLCDGPVNARATLLLAHGAGAAMSSPFMQHMAELLAERG
                     LRVARFEFAYMAARRAGGSRRPPPRAEALVDEYHAAISGIAAPGKLIIGGKSMGGRVA
                     SMIAEAQLHADKIAGLVCLGYPFHPPKSPEKLRVAHLAALTVPTLIVQGERDPFGTRA
                     EIAAYELSPAITFHWASDGDHDLGPRGGSGFTRKGNLAAAADAITAFALAR"
     gene            17071..18567
                     /locus_tag="PK264_05970"
     CDS             17071..18567
                     /locus_tag="PK264_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRD75473.1"
                     /translation="MSKRVLIAGFKHETNTFSIMPTDLAAYRARGLQRGTEIAETFTG
                     TNTELAAFMDAGKEHGWEVIPVISADATPSGKLTRECYEAITGEILDGIAKAGRLDAI
                     LLNLHGAMVAEHTFDGEGTLLERIREKVGRDVVIGVSLDLHANVTELMGRNADVMVAY
                     RTYPHIDLYDTGKEVAALVARTLAGKVKPRTIVARGRQMWGVDEGRTTAPGPMREALE
                     IARGIETKPGVLSCTINAGFPWADIPEVGPTAMIVVDQGKAGLDASHAADAKRLVDHI
                     WETRHRQTIKHMTIAEGIAVARAKGKPGAPVVLADFADNPGGGGYCDTPGLIARLIEA
                     GIEGAAMSLLYDPETTAQAVKAGVGARIDVKLGGKVDPAFGRPIEAKAEVRHVSDGRF
                     KITGPMMTGMSYDIGPVATIRIRGVDIVVGSKRMQNYDLGFFRIGGIEPKDRPVLAVK
                     SMQHFRAAYAPIASEIVIVDEGGGITSNDLKKLPFKNVRRPIYPLDLD"
     gene            19072..20325
                     /locus_tag="PK264_05975"
     CDS             19072..20325
                     /locus_tag="PK264_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HRD75474.1"
                     /translation="MPRYSIFALIGQGFARQTGWEPAWRKAEPKRAYDIVIVGGGGHG
                     LATAHYLAKEHGLTNVAVIERGPIGLGNAGRNTTIVRSNYLLPENSRFYEKSMQLWEG
                     LEADLGFNVMMSQRGVLNLFHTDGQRDAYARRGNAMRLEGIDAELLDTEGVRRFSPLI
                     DVDNGRYPVMGGLLQPRGGTARHDAVNWGFARAASARGVDIVEHCEVTGFRRVGNRIT
                     GVETTKGFIAARKIGLAVAGHTTQVLAMAGLSAPIETHKLQAWVSEAIKPMIDGVITF
                     GAGHFYISQSDHGGLVFGGGLDGYNSYAQRGTLPEAEHVMAAGISMIPALSRIRVLRQ
                     WAGIMDMSLDGSPFICKTPLEGLYLNAGWCYGGFKATPGSGWCFAHTIANDEPHALNA
                     AFTLERFREGRLIDEKGAGPFAWAH"
     gene            20342..20617
                     /locus_tag="PK264_05980"
     CDS             20342..20617
                     /locus_tag="PK264_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HRD75475.1"
                     /translation="MHQIRCPHCGVRDEYEFRYRGDASVTRPAADAGRNAFYDHVYVR
                     KDPKGWHIEWWHHVGGCRQWVKVVRNTLTHEIARTGTAGETLTVPTA"
     gene            20614..>21667
                     /locus_tag="PK264_05985"
     CDS             20614..>21667
                     /locus_tag="PK264_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD75476.1"
                     /translation="MSGPFRLEQGGRIDRARRLRFRFDDREYEGHPGDTLASALLAND
                     VTLLGRSFKYHRPRGMLAAGIEEPSALVEIGRDGRREPNQRATTVELFDGLEARSQNR
                     WPSLRHDFGAINQRLARFMPVGFYYKTFKWPSAFWERLYEPTIRRMAGLGTAASEPDP
                     DHYEHVHAHCDVLVVGSGTAGLSAARVAADSGARAILIEQDSEIGASLLLAPDLAEWR
                     RDMFAALGRQAETQLLPRTTAIALYDHNVVAAVERVGDHMAAVPKSSVRQRFWIIRAK
                     EIVLATGAIERLIAFPDNDRPGVMLSSAALSLAERYGVAVGRAPVLFTNNDEAYEAAF
                     ALARAGVVPRAIVDVRK"
BASE COUNT         3563 a         7538 c         7088 g         3418 t
ORIGIN      
        1 gcctacctgc agcgcgtcgg ccagatggag agcacgacga cacgcctcaa gggcatcctc
       61 gaggacgtga tcaagagcaa tcccgggaag acgcgcgacc agatcatcgc cctcttcgag
      121 gagcgcaaga aggcgttcgt gatggagctg cagaagcagg ccatcgccag cgcccgcgcc
      181 ggcgtcgttc cgacgaagcg caaggaagcc gagaacgaga tcatcgagga gcgcctcaag
      241 ctgcaggagg cgaagcgact cggagtcgtc gttacagacg acgatgtcga ccgcgtcatt
      301 aagcagatcg ccgacaagaa caagatgacg gtgccgaagt tcgccgagca catgaaatcc
      361 atgggcgccg acattgccgt catgcgctca cgcttccgcg cgacgctcgc ctggcgcgag
      421 gtcatccgcc gccgcttcgg tccgcagatt gccatctcga acaaggagat cgacgcgcat
      481 ctcgcaggtg cgggcgccgg tgccgcgggc gccgatagca tcctcctgca cgtgcaccgc
      541 ttcatcctgc cggtctccgg cgcgaaccag gcggctctcg cccaaagatt cgcggaagcc
      601 cagcgcatgc agaaggcgtt cgcgggctgc aaatcgtcgc cggggctcgt caaggccgcg
      661 gccggagcgc gtttcgagga tcttggcttc aagccgaccg gagcccttcc ggtgaaggaa
      721 gagacgcgca cgttcctgct caccgcgcgt gacggcgaga tggtgccgcc ctcgatcacc
      781 gccgcaggca tcgaactctt cgcagtctgt gcgcggcgcg tgacgagtgg cactgatgcc
      841 aagcgcgaga aggcccagga agagctaatg atgcgcgaat tcgagaccta ttcgaagcgc
      901 catctccgct tcctgctgca tgaagcccag atccatcggc agacgccggt ggcgccgggg
      961 aagtcgtgag ccgatcgcgc cgagccctgg ccctgacgct cggtgatccg tcgggccttg
     1021 ggccggacat cacgatcgcc gcctggcagc agcgccacac acacggaatt gcgccctttg
     1081 tcgccatcgg cgcacggcag gcgctcgtcg agcgtgcggg cgccttggga ctggcgatcc
     1141 cgatcgaagc cgtgacagac cccggcgcgg ccctcgcctg ctttgatcgg tcgctgccgg
     1201 tcnnnnnnnn nnatatcccg actgatgtac gtgtcgaggc agggcggccg accagcacga
     1261 acgcggaggc tatcgtccgc tcgatcgagc gtgccgtcgc gttcgccgag gccggtgaag
     1321 ccgcggccgt cgtcacctgt ccgatcgcca agagcgtgct gcagtcggcg ggcttcaagc
     1381 acccaggtca tacggaattc ctggcgcatc tcgccgagac gcgacggcgg ggcactacca
     1441 ttcggccggt gatgatgctc gccggccctg atctgcgcgt cgtgccgctg acgatccata
     1501 tcccgctcgc cgacgtgcca cgggcgatct cccgcccgct catcatcgag acgacgcgca
     1561 tcacggccga cgcgctccgc cgcgattttg gcctcgcatc acctcgcatc gccatcaccg
     1621 gcctcaatcc gcatgccggc gagagcggca cgctcggccg cgaggagatc gagacgatcg
     1681 ggccggcgat cggcgagctg aaggcgggtg gtctccacgt caccggaccg cattcggcgg
     1741 acgcgctgtt ccacgccgcc gcgcgcgcga cctatgatgc cgtgatcgcc atgtaccacg
     1801 accaggcgtt gatcccgctg aagacgctcg ccttcgatga cggcgtcaac gtcacgctcg
     1861 gcctgccgtt cgtgcgtacg tcgccggatc acggcacggc cttcgacatc gccgggacag
     1921 gggcggcgcg cccggaaagc ctgatcgccg cactcaagct cgcgagcgag atcgcaaccc
     1981 ggcgcgccgc tgcgcccacc cggggctgaa ccgagatgcc cgcgccgatc gttcgcgccg
     2041 tcagccgcaa cgccatctac ggcttcagca agcctgaagc cgagagcatt cgcctgatca
     2101 caggcctcgg cgtcgaagat gacatacatg cgggcgagac cgtcaagcat cgctctcggg
     2161 tcgcgcgcaa tccatccgca cccaacctgc ggcaggtgca tctcatccat gccgagctct
     2221 tcgatgaact ccgcgcgaag ggcttcctga aaccgcgccc cggtgcgctc ggcgagaatg
     2281 tgacgacagg cggcatcgat ctcctcggcc tttcggccgg cacgcggttg cggctcgggc
     2341 gcgaagccat catcgaggtg acggggctgc gcaatccctg ctcccagctc gatgccttcg
     2401 aagcgggcct catgggtgcc gtgctcgaga aatcgcgcga cggccgcctc gtccgcaagg
     2461 ccggcatcat ggcgatcgtg atcagcggcg gcggcgtcag agcaggagac gccatcgagg
     2521 tcgtcgcctt gccggccgtg cacgcgccac tcgagcccgt ctgaccgtga gcgacgagag
     2581 caagcccgat atcgcaaccg ccgccgaggc gtcgagagcc ggagcgagcc cagacggcct
     2641 gccgccgctg cgcgacgtga tttccgcgct cgacctgagg gcctcgaagg cgctcgggca
     2701 gaacttcatc ctcgacctca atctcacgcg acgcatcgca cgaactgctg gaacgctcag
     2761 cggccgcaca gttgtcgaga tcggcccagg ccccgggggc ctgacccgtg ccctgctgct
     2821 cgaaggcgct gagcgcgtca tcgtcatcga acgcgacgat cgctgccggc ccgcgctcga
     2881 ggcgatcggc gcacgctatc cgggtcggct cacgatccac atgggcgatg cgctggcaat
     2941 cgactggctc aggctcgtcg ccggcgcgcc ggacaaaccc atcatcgccg cgaaccttcc
     3001 ctacaacatc gccagccgac tgctcgtcgg ctggctcgag agcgagccct ggccgccgtg
     3061 gtggagccgc atgatcctga tgtttcagcg cgaggtcgcc gaccgcctcg tcgccgtgcc
     3121 ggggacgaag gcctatgggc gcctgtcggt gatctcgcag tggcgcgcgc gcgtaaagct
     3181 cgcgctgacg ctagggcccg aggcgttcac cccgccgccg aagatcgcgt ctgccgtcgt
     3241 cgacttctcg ccgatctcgg agcccgcttt cggctgctcg gcaacggcgc tcggcctcgt
     3301 cacgggggcg gccttcggcc agcggcgcaa gatgttgcgc cagagcctga agacacttgt
     3361 ccctgatccg gcggcgatcc tcgacgcggc cgggatcgca ggcgagctgc gcggcgagca
     3421 gctttccgtc cacgacttcg cgcgcctgac gcacgctgcc gagcgggccg gccttcagcg
     3481 ctgaggatca accacgcttg gcgcgcgccg cgatcttctc cgccatggcg atgaggcggc
     3541 acgccttcac gacgatcggc acgtctatca tccggccgtc gagctcggcc gagccgcgac
     3601 cctccgcgcg cgccttgtcg tcggcggcga tgacgcggcg cgcatgttcg agatcgaccg
     3661 ctgacggcgt aaagccatcg ttcaggatcg gcacgagtgc gggatgaatg caggaagaac
     3721 cgcggaagcc gaagcggcgt gagcggagaa ccgcggcctt gtagccttcg cggtctgaat
     3781 agtccgcgac cgagccgatg acgcccatcg gcatgagacc cgctgcgatc gccgcgatgg
     3841 cgacacgctg cttcggatag gtgagaactt ccgggtcggc cgccccgccg atctcaagcg
     3901 cgaaatcctc gctcccgagg ctcagtgcga caagtcgcgg gtcggcccgc gcgatctcgg
     3961 cagcttgctc gaaagcagac ggggtctcga tgagtgcgag aaacttcgtc tgaccgacag
     4021 gcacaccgcg cgccgcctcg acgctggcga cgagttcggc gagcagctgc acgtggctcg
     4081 cgctctcgac cttcggcagc atcagcgcgg ccaccccggg catcacggca gcctcgatgt
     4141 cgcgcacggc aagatcgagc ggacgattga tgcgcaccac gacatcggcg ccgttccggg
     4201 cgacacgcgg cgcggcctcc cgcacgagtg cgcgcgcatt cgccttctcc gctggcggaa
     4261 tgctgtcctc gaggtcgagg atgatcgcgt ccgccccgcg ctcgtgcgcg cgctcgacga
     4321 acttcggcac attcaccggc acgaacagca tcgagcgcca gacgggcagc tcgcgagtgt
     4381 cattcagcat gcttcggctc cggattgatt ttcttcgcgt cacttcagca tctgagtgcc
     4441 gtcgcgtcca tggctcggca accgaattgc catggagcgc ccgggcaccg gtcggtcggc
     4501 tcgaaccgac gcgaacgctg cctattgtgc gttggcagag ccattcccct ctgcgcctca
     4561 cctcaggatc gcgcgtcgac acctgaccgt gccgcaagct cccgcacagg cggatggccc
     4621 gggaaacgtc gctgtactcc tgcgccgcgc cctcgatggc gcgaggctcg acccgagcaa
     4681 gggctcactc aacgccaagc tgaatcgcgc cggctggaac gacgacgtcc cgctcgaaat
     4741 gctggatagg ttgagtcaaa tgcgcttggc gagcaaacgc tgtggccaac cgacgtctca
     4801 gagtatgccg aggggcgagg ccttcttcgg cggcgggaac gcgcgatcga gctcggccag
     4861 gtcttccgcc gtcagcacga ggtcggccgc cttggcgttt tcggcgacat gggcaagctt
     4921 cgaggctttc gggattgaga tcacgtgcgg gtgccgcagt ccccaggcga cggccaccgc
     4981 tgccggtgag acggtatggc gcgccgcgat cttggcgagc gccggattgc gcaggatcgc
     5041 accctggccg agcggcgaat aggccatgat tgcgacactc gccttcgcca tcgccgggat
     5101 gaggtcgaag tcgatgccgc ggctgccaag gtgatagagc acctggttcg ccgcccaggc
     5161 gcctggcttt gccacgcccg cgagctcggc catgtcgtcg acgtcgaagt tggagacgcc
     5221 ccagtggcgg atcttgcccg atgccttcaa ggcctcgaag ccggcgaccg tttcggcgag
     5281 cgggtaggag ccgcgccagt ggaggagata aagatcgatg cgatcggtct ttaggcgcgc
     5341 taggctgcgc tcgcaggcag cgatcgcgcc cgcgcgcgag gcattgtgcg gatagacctt
     5401 ggagacgatg aagacctggt cccgccgccc ggccacggcc tccgccacca cttcctcggc
     5461 accgccctca ccgtacatct cggcggtatc gatcagcgtg agcccgacgt ccagcccggc
     5521 ccggagcgcc ttcgcctcgg ccgcgcggtc cgatcgccgc tcgcccatgt gccaagtgcc
     5581 gagcccaagc gccggaactt tttcgccacc cggcagtgtc accatgcgca tggaggcctc
     5641 cctcgaatac aggctgcaac gatagttcgg ccctgggatt gcgccaagcg cccttgagaa
     5701 cggggcaccc tcgagattgg accgcttgcg ggcaccgcca acggggagcc agcagttgcc
     5761 gcttgctggc gccaaggtcg gcagtccggc cgcgcagacg ccggaaggcc cgttgggcca
     5821 tcggcacgtc gggcccgctt gccggcgcgc tcattgtgga ggacgtgccc ggcgcatcgc
     5881 gataactgtt acaatgttac atttcgatca ctctgccttg accgaaaatg tccagagcgg
     5941 agaccgacgc ttgcgaggta cgtcgaggcg agtcttgtta tgttattaca tgtctagctg
     6001 gaggtgcaga atgtgtctca cgtcaaggac gcgtgctata ggggtgctgc tcctggtgcc
     6061 gctcgccatg gcacccatcg cggccagagc gcagaccacg ctcccggggg tgacagtctc
     6121 gacaccgagc cccgtgacgc cctcaaaagg tgcgacctca ccaacccctc cctcgtccgc
     6181 ggatgcgacg tcgggcaagt ctccagcaaa ggccaagacg gccgcacccg ctcctgcggt
     6241 cacgccaagt gcgccgccgc ctgcgttgcc cctgcccggt acgctcatcg tcgctgatga
     6301 ttcctttgtt ccggtgacgg tgatccttga cggcgcgctc gcgcccaatc caagcgcgtc
     6361 acttgccgac ctgctctcga cgacgcccgg cgtcgcagct tcgacgttcg cacccggggc
     6421 gagccgtccg atcatccggg gactcgaaaa caatcgggtg cgcatccagg acaacggcgt
     6481 cgcctcccat gacgtctcag cgctcagcga agaccacggc gtgcccatcg atccgctggt
     6541 cggcggccgg gtcgaggtga tccgcggccc agccacgctg cgctacggct cgcagtcgat
     6601 tggcggcgtg gtcagcgccg agaacaaccg aatcccgacg atcattccgc cctcaggctt
     6661 cagcctcgag acgcggggcg gctggacctc cgtcaacagc ggtcgcgatg gcgggctgat
     6721 cgtcgacgtc ggaagtggca acgtcgcgat ccatgccgac gccttctccc gcgatgccgg
     6781 cgactatcgc acccctcacg gcgtgcaacc gaactcgagc ctccacgcgg acggcggctc
     6841 gctcggcggc tcgctcatct tcgacaaggg ctatttcgga gtcgcctata cccgttacgc
     6901 gagcctctat cgcatcccgg gtgtggaggc cgccgagcgc gcccttcgca tcgatctcga
     6961 ccaatggcgg ctgacgagca agggcgagtg gcgcgtcgag agcggtgccg tggaccgaat
     7021 ccgcttctgg ttcgggcgca ccgaatatcg ccatgacgag atctcgatcg acccggggac
     7081 cggcgagcga gagatcggct cgaccttccg caataccgag ctcgagggac gcatcgaggt
     7141 ccagcacagg cccttgctca tggcattcgg gcgcctcacg ggcgctgttg gcctccagct
     7201 cggcgatcgc cgcctgtcgg ccgcgggcga aggcggggag ttgctcgcac cgaaccgcac
     7261 ggacaccgcc gcgctcttca tcttcgagga gctacagccg acagacaggc tgcgcatcca
     7321 ggccgcggcc cggatggagc gcgcacgcgt gcgcgggacg gcggctctct ttcccgacac
     7381 attcctgcct ccaccggacg agccggacct cgaagggcgc aatcgcacgt tcaagccgcg
     7441 aagcgtaagc ctcggcatcc tccacgagtt gccactcggc atcgtcgcgc gcgttacagg
     7501 tcaactcgtg gagcgcgcgc ccgacgccgc ggagctcttc tcaaagggtc cccacgaggc
     7561 cacgcagacc ttcgagatcg gcaatccgaa cctcgccatc gagcgggccc gcaccttgga
     7621 gatcgggttc cgtcgcgcac gcggcgactt ccgctttgac gcgaccgcgt tccatacgga
     7681 gttcgatggt ttcatcttca agcgcctgac cggcgtcacg tgcgacgacg actttgcttc
     7741 atgcggtgcc ggaagcgaac tcgagcaact cgtttttggc cagagggacg ccacgttcca
     7801 gggcgtggag atcgccgccc aatgggacgt gatcccgatc tggcggggcg tgctcggcat
     7861 cgatggacag tatgattttg tgcgcgcgcg cttcgaggac ggcagtgccg ttccccgcat
     7921 cccgccgcat cgcgctggcg ccggtgtcta ctacaaggat ggacgatggt acgcgcgcgt
     7981 cgggtttctg cacgcattcc ggcaggacga gatcgcgctc ggcgagacgt ctaccagcag
     8041 ctactctctc ctcaacgcgg aactcagcta cacgatccgg ccctcggcaa ccgggcccgg
     8101 cgcacgcgaa attgtcatcg gcctgcgcgg cgacaatctt ctcgatgacg acgtgcgcaa
     8161 tcacgtctca ttccgcaaag acgaggtcct gcagccgggg cgctcgatcc gattgtttgg
     8221 taccctcaag ttcaactagc cgcagacccg gcgaaagccg ggcttgacca tgaaaggcga
     8281 agcggacata cgggtcgcgg gccacccctc cccaacgagg ggcaagctat ctggaaggat
     8341 aggaccatga ccacgtcccc gcgaccggcc aagcggccgg cgaacgcctg cttttcctcc
     8401 ggcccctgcg ccaagcgccc cggctggacg ccagaagccc tcggcaaggc gtttctcggc
     8461 cgctcgcacc gggcgaagga gggcaaggct cggctgaagc tcgcgatcga taagaccgcc
     8521 tcggtcctgg gcctgcccca aggctatcgt acagccatag ttcccggctc cgacaccggc
     8581 gcctacgaga tggcgatgtg gacaatgctc ggcgcccgcg gtgtcgacgc gctcgcctgg
     8641 gaaagcttcg gcgcgggctg ggtgaccgac atcacgaagc agctgaagct cgccgatgtg
     8701 cgtgtgctcg aggcgcccta cggcacgctc cccgacctgg cgaaggtcga ttttagccgc
     8761 gacgtgctct tcacctcgaa cggcacgacc tcgggcgtgc gcgtgcccaa ctacgactgg
     8821 atcccggctg atcgccaggg gctcacgttc gccgatgcca cctccgccgc cttcgcgcag
     8881 ccgatcgact ggacgaagat cgatgtcgcg accttctcgt ggcagaaggt gctcggcggt
     8941 gaggcggcgc acggcatgct ggtgctctcg cctcgcgccg tcgagcggct cgagacctgg
     9001 aagcccgcct ggccgctgcc gaaggtgttc cggctcacca agagtggcaa gctcgaagcg
     9061 ggcgtgttcg cgggcgagac gatcaacacc ccgtccatgc tgtgcgtcga ggattatcta
     9121 gatgccctcg cctgggccga gggcgtcggc ggcgccaagg ggttgaacgc gcgctcggac
     9181 cgcaacgcca aggcgctgtt cgactgggtc gagcgcacgc cctggatcgc caatctggcg
     9241 gtcgatccgg caacgcgctc gaacacgtct gtctgcctga agatcatcga tccggcgatt
     9301 gcggctcttc ctgccgagaa gcaagcggcg ttcgcgaagg accttgccgc gctcctcgag
     9361 aaggaagggg ccgcactcga catcggctcc taccgcgatg cgcccccggg cctcagaatc
     9421 tggacgggtg cgacggtcga gacagccgac atcgaggcgc tgctgccctg gctcgactgg
     9481 ggctttgcca cgctcaaggc cgcgctcgcg aaagccgcct gatctgaaat ggtgtgccgg
     9541 acctcgtccc gctgccgtcg aggcacgccg gggcggggct ctttgcctca tgtcgcctgg
     9601 ccacgcccgc cccgtacatg agtcatcgtg acgagggttg gctctggcca agggagtgag
     9661 aggcgggtgg cgccgaaaga cgaaatcagc acgatcgatg aaagcgggag cgaaaagaag
     9721 ccccgcaacc tccggccgct cgccggtctg gcgccgcagc tcctgcgcca tcggggcgcg
     9781 cttgcaggcg ccgccgcggc gctcgtcgtc tcggcgctcg ccatgctcgc catgccggtc
     9841 gccgtccggc acatgatcga tcagggcttc tcgggccgcg acgggggctc gatcaatcgc
     9901 tacttcctga tcttgatcgg catcggcctc gtgctggcag cagcaagctc gacgcgcgcc
     9961 ttcctcgtca actggctcgg cgagcgcgtc gtcgcggacg tgcgccgcga tgtcttctcg
    10021 catctgacga cgctctcgcc ggccgccctc gagacggccc gctcgggcga gatcctctcg
    10081 cggctgacgg ccgatacgac cctcgtcaag tccgccgcgg gctcggcgct gagccaggcg
    10141 gtgcgcaatc tcatcatgct gatcggcgcc gtggcgatga tgttcgtgac gagtccgacg
    10201 ctgaccttgg tgatgctcgc ggcgatcccg gcgatcgtac tcccgctgat gggtatcggg
    10261 cggatcgtcc agcggctgtc gcgccgcgcc caggacacgc ttgccgaggc ctcggcctat
    10321 gccggcgaga acctgtcggc cgtgcgcacg atgcaagcct acacgcacga gcgcacggtc
    10381 gttgcccgct tcacgtccgc ggtcgaggcc gcgttcgccg cctccgtcga ccggctgaag
    10441 gcgcgcgcag gcctcgcggc gctcacaatc gccctcatcg tcggcagtat cgtcggcgtg
    10501 ctctggtatg gcgcgacgat ggttgtcgcg aacgagatga ccggaggtcg gctcggccag
    10561 ttcgtgctct acgccctttt tgcaggcggc gcgctcgccg agcttgccga ggtctggggt
    10621 gaggtctcgc aggcggcagg cgccgcggaa cgcctcgccg agcttctggc actcgcaccg
    10681 ggcatcacgg cgccgcgcga tcccctgccg atgccgaacc cggcgcgagg cgcaatcgag
    10741 ctcgaccggg tctcgttctc atatccctcg cgcccggagg tgaaggcgct cgatcacatc
    10801 atgctcagga tcgagcccgg cgagaccgtc gcgatcgtcg gctcgtccgg ggccggaaaa
    10861 tcgacgattt tcaatctgtt gctgcgcttc tacgacccga ccgccggcga ggtccgcgtc
    10921 gatggcgtgc cgacgcaacg caccgacccc aaagcgctgc gcgagcgctt ctcgatcgtg
    10981 ccgcaggagc ccgcgctgtt cgccgacacg gtcgccgaga acatccgcta cggtgcgccc
    11041 ggcgcgagcc ccgccgatat cgagcgcgcc gcggagcttg ccggcgcgct cacgttcatt
    11101 cgcgcgctcc ccaagggctt tgagacggtc ctcggcgagc gcggcacgac gctctcgggc
    11161 ggccagcgcc agcgcgtcgc gatcgcgcgc gcaatcctgc gcgatgcgcc gattctgctg
    11221 ctcgacgaag cgacgagcgc gctcgacgcc gagagcgagg cctccgtcca gcaggcgctc
    11281 gacgcggtga tggcaaaccg gacgacgatc gtgatcgcgc accggctcgc gaccgtgctc
    11341 aaggcgaagc ggatcatcgt catggacagc gggcgcatcg tcgaggaagg cacacacgcg
    11401 gcgctcgtgg ccagaggggg cctctacagc cgccttgcgg cgctccagtt ccgcgccgcg
    11461 gccgagtagc ggcggaccgc cgatccctac gaccggaggc gcccgctgaa gcgcacggcc
    11521 tcggcttcgc cctgtccctt cgcctggctc ttgacgccgc ggcccggctc cacctcgccg
    11581 attgcgaccg gcctctcgcc tgccgccgaa agcgccgaag tcacgtcagc cacccgcgcc
    11641 gggtcggcga ctaccaccat gccgatgccg cagttgaacg tgcgcagcat ttccgtgtcg
    11701 ccgatccccg cggccttggc gagccagccg aacaccggcg gcggcgagac cgtatcgagc
    11761 cggacgtgcg cggcgagccc cttcggcatg acccgcggca ggttctcggc gagcccgccg
    11821 cccgtgatgt ggcagagacc tttcacagcg ccatgtggac cagagccgcc cgtcgcgcgg
    11881 atggctgaga gcaccggctt cacgtagagc cgggtcggcg cgaggagcgc ggccgcgagg
    11941 tcgccgctcg cagcgaaggg cgcaggtgcc gcccacgcaa gcccttcccg ctcgacgatg
    12001 cgccggacga gagaatagcc gttcgaatgc acgcccgacg aggcgaggcc gatcaggacg
    12061 tcgccaacgg cgatatcgtg ccgcggcagc acctcgccgc gctcgacggc ccctacggca
    12121 aaaccggcga ggtcgtagtc gccagccttg tacatgccgg gcatctcggc cgtctcgccg
    12181 ccgacgagcg cgcagccgga ctcgcggcag ccttccgcga tccctgcgat caccgttcgc
    12241 gcgacctcga cgtcgagccc gccggttgcg aagtagtcga ggaagaacag cggctcagcc
    12301 ccctgcacga cgagatcgtt gacgcacatg gcaaccagat cgatgccgat cgtggcgtgt
    12361 cgccccgtat cgatggcgat cttgagcttg gtgccgacgc cgtcgttggt cgcaaccagg
    12421 atcggatcgc ggaacccggc gcgtttgagg tcgaacaggc cgccgaagcc gccgagatcg
    12481 gcgtccgcgc ccggccggcg ggtcgccttc accagaggct tgatggcctg gatgagcgcg
    12541 ttgccggcat cgatgtcgac gcctgaatcg tggtaggtga gcggcggctt gccgctgccc
    12601 gcgccggacg ctttcgttgt cttcgtcatc gatcaggctc ggttgggggt cgcgcgaccc
    12661 gattggccga ggcaatctgg cggacgagtt cgcgatatct gcgaaccccc aaccctagat
    12721 ttgccccatt ggtcggtggt catcgccgca atagcagagg gcgccgcccg gcgccatctt
    12781 ggcattgaat ggaggggagc attgagagga tcggcacatg ctggggggcg gcgcaccgtg
    12841 cgccgcttgc cccgagtgct cacggcgctc ctcgtggctg ctcagctgca gggtagcgcc
    12901 tttgcagcgc cagcggacaa ggtgttcgtc gtcggcagct atccggtcga ggcctcggct
    12961 ggtgacgcga ccacagcgaa ggagaaggcg ctcgcggaag gccagcaggc cgccttccgg
    13021 tcgctcctga agcggctcgt tcccgtcacc tcctataacc gcctgccgcg cctgaagaac
    13081 gtgaaggcgg ctgagctgat ctcgggttat gccgtccgct ccgagcgaaa ctccgacaca
    13141 gattacgtcg cgacgctcga cttcaccttc caggccgaag cagtgcgcaa cctccttcgt
    13201 cgcgagggcg tgcccttcct cgacgagcag gctccgcaga cgatcgtcgt ccccgtctgg
    13261 cgcgccgcga aggccgcggg gccggctgct tccaatgcag ccctcgccga tgccaaggct
    13321 gcgccggtct gggaagatgt atggaaaggg ctcgacctcg agaattcgct cacccccctg
    13381 aagctcgaga ccaccaaggc tgacctcaag cccgacacgg tcaaaggcct cgtcgacggc
    13441 cagctcgacc tcatccgatc gatcgcggcg agccatcgcg ccgagcgtgt catgctggcg
    13501 gtggccgagc ccgacatctc gggcaagaag cttatcgtca ccatcgtcgg acaggacgcg
    13561 gtcggcgcct tccatctgca gaaggcgcat aagctagagg gcagcgagct cgcctacacc
    13621 gccgagctcg cggccgtgat ctccctcggc atcatggaag gacgctggaa ggccgtgcga
    13681 ctgagaggcg gcgccgccgc ttccctgccg cgacccggtg gaccggcgct caccggcggc
    13741 cctgccggcg ccgacaccgt gatccacgtc gagttccgcg gcatgggcga gtggcagcat
    13801 atcagccgtg cgctctccgc aacgcccggc gtagagaacc tcgatgtcct cggcctttcc
    13861 gcgcgtggcg cgcgcatcac gctcgcctat cccggcgggc ccgaggcgct cgctcaggtg
    13921 ctgcgcaacc agcggatcat cttgcacaac actacgcaag ggtggaagct tcgggcggaa
    13981 tagcgcgacg gctcgcaggg gcgagcccgc cctgccacgg agcccaccat gaaaggcgtc
    14041 gtctttcccg gcaatcgtca gaccgagatc cgcgactttc ccgatccgac ccccggcgag
    14101 gaggaggtcg tgctcgagat caaggcctcg ggcatgtgcg gctccgatct ccacatctac
    14161 cgctcggcgg gcggcggggc ggcgcaagcg cgcgcgctcg gcctcggcgg ggaaggcggc
    14221 gaggtgatcg ccggccacga gccatgcggc gtggtcgtcg cgcgcggcaa aaacgtcggt
    14281 gaccggcagg cgcgcctcgg cgcccgcgtc atgcagcatc attatcacgg ttgtggcacc
    14341 tgccccgact gtgtcgacgg ctggtcgcag atgtgcggcg cgcccggcaa aaccgtctac
    14401 ggcatcaccg gtcacggcgg gcatgcaaag tacatgaagt gccccgccca cacgctgatc
    14461 gcgctccctg acgcgctttc cttcgaaacg ggcgccgcca tctcctgcgg caccggcact
    14521 gcctacggcg cgctgcgccg gctcgacatg aacggcggca agacgctcgc ggtgttcggc
    14581 caagggcccg tcggcacgtc cgccaccatg ctcggcaagg caatgggtgt ccgcgtcatc
    14641 gccctcgacg tctccgacga gcgcctcacg ctcgccaagg cctgcggcgc ggacgcaacc
    14701 gtgaacccca caaaggtcga cgcggtcgaa gcgcttaagt cgctgacagg aggccacggt
    14761 gtcgactacg cggtcgagtg ctcgtcggcc gagaccgccc gggcggcgat ccgcggcgcg
    14821 cgcagctggg gcaaggtctg ctttgtcggc gagggcggca cggtctccat cgatgtcagc
    14881 ccggacatgc tgcgcaaaca gctgacgctc ctcggctcct ggaccttcaa caagcagcgc
    14941 caggacgagt gcgcgcgctt catcgccgcg aacaaggtgc cgatcgagac gctgatctcg
    15001 gaccgctact cgctcggcga ggccgaagcc gcctacaaga agttcgacaa gcagaccatg
    15061 gggaagggca tgatcctgcc cgcgtagcca gcgatttcct gaggcgcact atggcgcctc
    15121 agggcttcgt gacgatgaag tcggtgcccc agccgcgcgc cgtctccggg ccgaggccct
    15181 gatcggagat tatgagcgag gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnngagagcg cgcccgccgc agtcgcgtcg gccgtcgggg gcgcggcgtc
    15301 ggcatccgtg gtctggccgc gtcgcttcag ggccgcgacc cgctcgttgc cccgcgtctc
    15361 gcgaccgctc gagaccgtca ccacggtcca gcgcatgccg tcgccaccat cggtcagctc
    15421 ggccgccgag aagacatgtg tgccgatcgg ctgctcgggc gcttcgatcg tgaccggcgc
    15481 atcgaagatt ggatggaagc cccgccggac gtagacggtt ttgtccttgc ggctgacgaa
    15541 gatcgacaga tgacgatcag gcgacggccc cttcgtgtcg ggatcgcgcg tgtcggcctc
    15601 ggcgcggcga agagcttcct gctcgtcggc cggcaggggg cgcgcgacga agagatgggg
    15661 gtggcggatg tccaccggcg caaccggcgc atcgaccacg acgacgcgat ccccgacctt
    15721 ggtcgcctcc caaagcgccg ccgcgaacga ggacggcagg cggatgcagc catgcgaggc
    15781 gcgatagcct ggcaggtggc cctcgtggag cgcgatgccg ctccaggtca gacgctgcat
    15841 gaagggcatc ggcgcgccgc tgtagatgtt cgactcgtga tagcgccgct tctcgaggac
    15901 ggagtagacg ccgcgcggcg tctcgtagcc gtcgcgacca gaggaaatgg ccgcttccga
    15961 ccaatgccgg ccagcggcgt agaccgtcac cttctgcgtt ccgagcgaga cgacgatggt
    16021 gagcgggcct tccgggcgga ctggcgtgtc acgcacgacc ttggtcgccg ggcgcaagga
    16081 ctgcgccgca gcggtcggca ctccttcatc gcaggcggcc aagccgacaa atacggccac
    16141 ggaccatttg agcaacatgc ctgtcgtcga cgtcgcgcgc caagtgttcc accgcatcat
    16201 tttggtactc gcaagactac gacacccctc cgttctggaa taacagcatt gcgcgccgct
    16261 gtccgcgatc gaaatccggc atgtgcgatg cagcccgacc attttccttt accggcgtga
    16321 tctcatcgca tcatgctttc ggatcgacgc cccgatgagc cggagcctgc aatgtcccca
    16381 cctgagtact tgtgcgacgg cccggtcaat gccagggcca cgctgctcct cgcccacggc
    16441 gccggcgctg ccatgtctag ccccttcatg cagcacatgg cggaattgct ggcggagcgc
    16501 ggcctccgcg tcgcgcgctt cgagttcgcc tacatggcgg cgcgccgcgc gggagggtcg
    16561 cgacgaccgc cgccgcgcgc cgaggccctc gtcgatgagt atcacgcggc catctcaggt
    16621 atcgccgcgc cgggcaagct catcatcggc ggaaagtcga tgggcggccg ggtcgcgagc
    16681 atgatcgccg aagctcagct ccatgcggac aagatcgcgg gcctcgtctg tctcggctac
    16741 ccgttccacc cgccgaaatc tcccgagaag ctccgggtcg cccatctcgc tgctctcacc
    16801 gttccgactc tgatcgtcca gggcgagcgc gatccctttg gcacgcgcgc ggagatcgcg
    16861 gcttacgagt tgagccccgc catcaccttc cactgggcga gcgacggcga tcacgatctc
    16921 ggcccccgtg gcggctctgg cttcaccagg aagggcaatc ttgccgctgc cgcggacgcg
    16981 atcaccgctt tcgcgcttgc gcgataggtc gagctgggct cctctcgctg ccgggtccgg
    17041 cgccacacgc cggcacgttg ggagacagcc atgtcgaagc gcgtactgat cgccgggttc
    17101 aagcacgaga cgaacacgtt ctcgatcatg ccgacggacc tcgccgctta ccgcgcccgc
    17161 ggcctgcagc gcggcaccga gattgcggaa acctttactg gcacgaacac ggagcttgcc
    17221 gccttcatgg atgccggcaa ggagcatggc tgggaggtga ttccggttat ctctgcggat
    17281 gcgacgccct cgggcaagct cacgcgcgag tgctatgagg cgatcaccgg cgagatcctc
    17341 gacgggattg ccaaggccgg ccggctcgac gccatcctcc tcaatctgca cggtgccatg
    17401 gtcgccgagc acacctttga cggcgagggc acgctgctcg agcgcatccg cgagaaggtc
    17461 ggacgcgatg tcgtgatcgg cgtgtcgctc gatctgcacg ccaacgtcac cgaactgatg
    17521 gggcgaaacg ccgacgtgat ggtcgcgtac cgcacctatc cgcatatcga tctctacgac
    17581 acggggaagg aggtcgctgc cctcgtcgcc cgcacgcttg cgggcaaggt gaagccgcgc
    17641 acgatcgtcg cgcgcggccg ccagatgtgg ggcgtcgacg aaggccgcac gaccgcgccg
    17701 ggccccatgc gcgaagcgct cgagatcgcg cgtggcatcg agacgaagcc gggcgtgctc
    17761 tcctgtacga tcaatgcggg ctttccctgg gccgacatcc ccgaggtcgg gccgacggcg
    17821 atgatcgtcg tcgatcaggg gaaggccggc ctcgatgcct cccacgccgc cgacgccaag
    17881 cgcctcgtcg accacatctg ggaaacgcgc catcgccaga ccatcaagca tatgacgatc
    17941 gccgagggca ttgccgtcgc ccgtgccaag gggaagccgg gcgcgcccgt cgtgcttgcc
    18001 gatttcgccg acaatcccgg cggcggcggc tactgcgata cgccgggcct catcgcccgc
    18061 ctgatcgagg ccggcatcga gggcgcagcc atgtcactcc tctacgatcc cgagacgacg
    18121 gctcaagccg tcaaggctgg tgtcggagcg cggatcgacg tgaaactcgg cggcaaggtc
    18181 gatcctgcct tcggtcggcc gatcgaggcc aaggccgagg tgcgccacgt ctcggatggc
    18241 cgcttcaaga tcaccgggcc gatgatgacc ggcatgtcct acgacatcgg cccagttgcc
    18301 accatccgca tccgcggcgt cgacatcgtc gtcggctcga aacgcatgca gaactacgac
    18361 ctcggcttct tccgcatcgg cggcatcgag cccaaggacc gccccgtcct cgcggtgaaa
    18421 tcgatgcagc acttccgggc ggcctatgcg ccgatcgcct ccgagatcgt gatcgtcgac
    18481 gagggcggcg gcatcacctc gaacgatctg aagaagctgc cgttcaagaa tgtccgccgc
    18541 ccgatctacc ccctcgacct cgactgagac gatcgcaaca atcgcgctcg atgatggccc
    18601 gcgatttgct ctgcaacggg gccgcaggct cggacccggc ctagttgcgt tgatcccatc
    18661 gccgcccacc ccgccgccag ggccgcgtcg gcgcctcatg cggcaccgct ggccaaggtg
    18721 gtgcccacgc ccgaagccaa ttgcgacatc cttcgcggcc gtgacacgag gagagagccg
    18781 gtcaaactca caccacagcc tatgctcaat gttagctcgg tcgtcaggct cacgttcgat
    18841 gatctgaatt tcagcggcgg agaatcgata gcgtcctgaa gtgagccgat agaccgcaac
    18901 gcccgcatca cggggctcga gcgcgtcgac acctgaccct gccgcaagct cccgcacagg
    18961 cggatggccg gcatgccttg aacggcgagg ctcagctgct agtgtgattg cgtgggcgcc
    19021 ctgcccgtag acacgcggct tctccccatc cggtcacaca ctttcgatct catgccccgc
    19081 tattcgatat tcgctctcat cgggcaaggc tttgcccgcc agacgggctg ggagcctgcc
    19141 tggcgcaagg ctgagcccaa gcgcgcctac gacatcgtca tcgttggcgg cggcgggcac
    19201 gggctcgcga cggcgcacta tctcgccaag gagcacggcc tcacgaatgt tgcggtcatc
    19261 gagcgcgggc cgatcggcct cggcaatgct gggcgcaaca cgacgatcgt gcgctcgaac
    19321 tacctcttgc ccgaaaacag ccgcttctac gagaagtcga tgcagctctg ggagggcctc
    19381 gaggccgacc tcggcttcaa cgtgatgatg agccagcgcg gtgtcctcaa tctcttccac
    19441 accgacggcc agcgcgacgc ctacgcgcgg cgcggcaacg ccatgcgcct cgagggcatc
    19501 gacgcggagc tgctcgacac ggaaggcgta cgccgcttct cgccgctcat cgacgtcgac
    19561 aacggccgct atccagtcat gggcgggctg ctgcagccac gcggcggcac cgcgcgccat
    19621 gacgccgtca actggggctt cgctcgagcg gcctccgccc gcggcgtcga catcgtcgag
    19681 cattgcgagg tgacgggctt ccgccgcgtc ggcaaccgca ttaccggcgt cgagacgacc
    19741 aagggcttca tcgccgcgcg caagatcggt ctcgcggtcg cggggcacac gacccaagtc
    19801 cttgccatgg ccgggctttc cgcgccgatc gaaacgcaca agctgcaggc ctgggtctcg
    19861 gaggcgatca agccgatgat cgacggcgtc atcaccttcg gcgcagggca tttctacatc
    19921 tcgcagtcgg accatggcgg gctcgtcttc ggcggcgggc tcgacggcta caattcctat
    19981 gcccagcgcg gcacgctgcc cgaggccgag cacgtcatgg cggcaggcat cagcatgatc
    20041 ccggcgctga gccgcatccg tgtgctccgc cagtgggccg gcatcatgga catgagcctc
    20101 gatggctcgc ccttcatctg caagacaccg ctcgaggggc tctacctcaa cgccggctgg
    20161 tgctacggcg gtttcaaggc gacgcccggc tccggttggt gctttgccca caccatcgcc
    20221 aatgacgagc cgcacgccct caacgcggcc ttcacactcg aacgctttcg agagggtcgc
    20281 ctgatcgacg agaagggcgc cgggcccttc gcctgggcgc actgaaggat aaagccgagc
    20341 catgcaccag atccgctgcc cccattgcgg cgtccgcgac gagtacgaat tccgctaccg
    20401 cggcgacgcg agcgtcaccc gaccggcagc cgacgcagga cgcaacgcct tctacgatca
    20461 cgtctacgtc cgcaaggacc cgaagggctg gcacatcgag tggtggcatc atgtcggcgg
    20521 ctgccggcag tgggtgaagg tcgtgcgcaa cacgctgacg cacgagatcg ccaggaccgg
    20581 aaccgccggc gagaccctca cggtgccgac agcatgagcg gcccgtttcg cttggagcag
    20641 ggcggccgga tcgaccgcgc gcggcggctg cgcttccgtt tcgacgatcg cgagtacgag
    20701 gggcatcccg gcgacacgct cgcctcggca ctcttggcca acgatgtcac gctcctcggg
    20761 cggagcttca agtatcaccg cccgcgcggc atgctcgccg ccggcatcga ggagccgtcg
    20821 gccttggtcg agatcggccg cgacggccgg cgcgagccca atcagcgcgc gacgaccgtc
    20881 gagctgttcg atggcctcga ggcgaggagc cagaaccgct ggccctcgct ccgccacgac
    20941 ttcggcgcga tcaatcagcg cctcgcccgc ttcatgccgg tgggcttcta ctacaagacg
    21001 ttcaagtggc cgtcggcctt ctgggagcgc ctctacgagc cgacgatccg gcgcatggcg
    21061 gggctcggca cagccgcgag cgagcccgat cccgatcact acgagcacgt ccacgctcat
    21121 tgcgacgtgc ttgtggtcgg cagcgggacc gctggtcttt cggccgcccg tgttgccgcc
    21181 gacagcggcg ctcgcgccat actgatcgag caggacagcg agatcggcgc gagcctcttg
    21241 ctggcgcctg atctggcgga gtggcggcgc gacatgttcg cggcactcgg acgccaggcg
    21301 gagacgcagc tccttccgcg cacgacggcg atcgcgctct acgaccacaa cgtggtcgcc
    21361 gctgtcgaac gggtcggcga tcacatggcg gcagtgccga aatcgagcgt acgacagcgc
    21421 ttctggatca tccgggcaaa ggagatcgtg ctggcgacgg gcgccatcga gcggctgatt
    21481 gcgttcccgg acaacgatcg cccgggcgtg atgctctctt ctgcggcgct ctcgctcgcc
    21541 gagcgctatg gcgttgccgt gggccgcgcg cccgtgctct tcacaaacaa cgacgaagct
    21601 tacgaggcgg ctttcgcgct cgcccgtgcg ggcgtcgtgc cgcgcgccat cgtcgacgtg
    21661 cgcaagg
//
LOCUS       DAORNH010000037        20980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_1963_length_20980_cov_2.703608, whole genome shotgun sequence.
ACCESSION   DAORNH010000037 DAORNH010000000
VERSION     DAORNH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20980
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_20980_cov_2.703608"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK264_05990"
     CDS             <1..241
                     /locus_tag="PK264_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HRD75477.1"
                     /translation="ADLDELKKKGVNIVRTPDSVLEAQLKAWDKVIAAQSADAKTGAF
                     FAKVIASQKAWVKRSAGYMQANNVPTTMAYKHFFG"
     gene            331..924
                     /locus_tag="PK264_05995"
     CDS             331..924
                     /locus_tag="PK264_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD75478.1"
                     /translation="MNRLLFLVDALSSFVGKLFAWCILLLTFAICYEVVARYAFLAPT
                     DWAYDVSYILYGTLFMMAGAYTLSRNGHVRGDFLYRTWQPRAQAWSDLILYFVFYFPG
                     ILALIYAGWKYFHLSYLMNEHSSASPNGPVIWPFKGVIPLTGILLLLQGLVEVARCII
                     CIRTGEWPQRLHDVEELEKVILEKAQADQAAAPGAKP"
     gene            921..2411
                     /locus_tag="PK264_06000"
     CDS             921..2411
                     /locus_tag="PK264_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD75479.1"
                     /translation="MTLDQLLRSVISPLISDPAMGILMLLLFLVFLMLGFPIAFTLMA
                     MGIGFGYYARGDSVFQLFVQKTFGVMSSDVLLSIPLFLFMGYMVERANILDRLFRSIQ
                     LAAGWLPGSLAVATLVTCALFATATGIVGAVVTLMGLLAFPAMLRAGYDTRLAAGVVC
                     AGGCLGILIPPSVMLILYGATAGVSVVKLYAAAFIPGVMLAGLYILYVVVLAMLKPEL
                     APKLPPQERNAPIGRVIWELATSFFPLALLILGVLGAIMFGFASPQEAAAVGALGALL
                     LAIAYRSFSFEKLKESVYLTARASAMVCWLFVGSAVFSAVFAQLGGQKVVEDFVLSLN
                     LSPFWFLIMTQVIIFILGWPLEWTEIIIIFLPIFLPLLDDFCIDPMLFGVLVALNLQT
                     SFLSPPVAMAPFYLKGVAPKHVTINQIFKGVYPFLYIVVLSMVLFYAFPQLGTYLPEK
                     LYSHRPPKCAIQPKKAEAQPPIEGAALAQPGATVASCPWRSSYKPV"
     gene            2422..3777
                     /locus_tag="PK264_06005"
     CDS             2422..3777
                     /locus_tag="PK264_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD75480.1"
                     /translation="MAKQSFIDLSATEASALLSRGEVSAVEYVTACLERIAEREEEVR
                     AFEHLDAEHALRQAKAADEARAIGLGVGPLNGLPVGLKDIIETADMPTGNGSPIFKGY
                     QPTRDAVCVSQLKTAGAVIMGKTVTTELATLVPGKTRNPHNLAHTPGGSSSGSAAAVA
                     DRMIPLALGTQTGGSVIRPASFCGIFGLKPTLGLISRRGVTMQSHTLDTVGVYGRSVE
                     DLGLVADALSAHDPDDEVSYPRARPSLAATLAEDPPVPPLLAFCKTHVWDQAEAATKD
                     AFAELVATLGNRVQEVDIPALELARKAQPVVQGTENAAYYGPLLDKYPEGVAPALADR
                     IRAGRKFSGEEYVRALRDRETIYAAVERTLVDFTAILTPASCGPAPKGLKSTGNPVFN
                     ATWTLLGVPCVTLPLLDVDGLPLGVQLVGRRRDDGRLLRTARWLARELAPKPRKRKSK
                     A"
     gene            complement(3788..4516)
                     /locus_tag="PK264_06010"
     CDS             complement(3788..4516)
                     /locus_tag="PK264_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD75481.1"
                     /translation="MIWAAILVIGIAAGTLGGIVGFGASVMLMPILVLGFGAKEAVQI
                     MAIAGIIANVSRVVVWWRDVDVRLNVVYCATALPAAYFGARTLLALDTRMVETILGVF
                     FIVMIPVRRWLLAKGFKVGLPHLAVVGAGIGFLTGIVASTGPLNTPFFLAYGLTKGAF
                     LGTEALGSALIGITKAITFRSFGSLPVETISKGCAVGASLWIGSWIAKRLVERMDASQ
                     FRFVIDAMMALAGLTLLYGALTAA"
     gene            complement(4523..5089)
                     /gene="hslV"
                     /locus_tag="PK264_06015"
     CDS             complement(4523..5089)
                     /gene="hslV"
                     /locus_tag="PK264_06015"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HRD75482.1"
                     /translation="MSATMSGDAGQTQWHGTTILTVRKGGRVVIAGDGQVSLGATVIK
                     ANAKKVRSLGRGDVIGGFAGSTADAFTLFERLEAKLEQHPGQLVRAAVELAKDWRTDR
                     FLRRLEAMMIVADRGKTLVLTGTGDVLEPEFDVMGIGSGGMYALAAARALIDQPLDAE
                     AIARKAMGIAADICVYTNRNLVVEALSA"
     gene            5174..5797
                     /gene="hisB"
                     /locus_tag="PK264_06020"
     CDS             5174..5797
                     /gene="hisB"
                     /locus_tag="PK264_06020"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRD75483.1"
                     /translation="MQEQSGRRAVARQATIERSTKETSIKVTVDLDGTGRSRIATGIG
                     FLDHMLEQVARHGLIDIDLEAKGDLHIDFHHTTEDSGIALGQAIAKALGAKQGITRYA
                     SAYLPMDEALTRVAIDVSGRPFLVWDVTFSRPKIGEMDTELFREWFQAFAMNAGITLH
                     VATLYGDNNHHIAESCFKGLARSLKDAIAIDPRQAGRVPSTKGTLTG"
     gene            complement(6026..7822)
                     /locus_tag="PK264_06025"
     CDS             complement(6026..7822)
                     /locus_tag="PK264_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75484.1"
                     /translation="MHSNDPRDVHDIWRQGLIERLDSIERNAALPPPAPLAARAAPAG
                     RLAFPTPPADASFERILEETLRIVENAPMLPPAATPVVGQREAGYRDARHAQAALPPP
                     AHRPVATDAAHRPVPGNSLPRPVDRELATIPAPAPMLAEPLPRGILPTQPATHSEVHR
                     PSADPIEAALDAVTTQLFGIKPPPKAGLQIGRVPNATAAPSFDADERLALRGQGTSFD
                     ELHSAIASARGAEAVPASRRGGRLWAFAGVGAAVAFGGLAYLVQVGGPLDTLAGTSSN
                     KPVAKSTVAKAAAPERTGVDGDRAAHHWQVIAEERPHAVFFDPDHAHASSDHGRVRIG
                     FELHVRAEDRRAAPIPLDLKWHDGIEPRAVSILISNLPAGVTLSHGKPTQTGAWIVAS
                     SDLGALRVSLPDDVKHLELTVDAIDEHGTRLARARALLKTVEMVAAAPVQPPTSSGAK
                     RKPSKTADAVVPPAADVTDATPPLTIATKGGAQPKSDVSAVPVPIMSSARVHEPRPIA
                     KDGAAPTLPPAAAPAPSVAAAPVSPKAVAKAPRPAGASALGGPMESEASSPVAPGQPA
                     APLPAAPTSLGSFLPPPPPVSTAPVWVPRPDH"
     assembly_gap    8293..8328
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8329..9131)
                     /locus_tag="PK264_06030"
     CDS             complement(<8329..9131)
                     /locus_tag="PK264_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75485.1"
                     /translation="MSTAAPATTGTAAAQRPSSPATAAENQPILSVNNIEVIYNHVVL
                     VLKGVSLQVPKGAIVAILGANGAGKTTTLRAVSNLLHGERGEVTKGSIVFDGEEVHRE
                     SPNALVKKGCIQVMEGRHCFAHLNVEENLLTGAYTRRDGRGAVKADLEMIYNYFPRLK
                     ERRTSLTGYISGGEQQMTAIGRALMSRPKMVLLDEPSMGLAPQLVEQIFEITKRINQE
                     QGVSVLLAEQNTNMALRYATYGYILENGRVVMDGTAEALRENADVKEFYL"
     gene            complement(9131..10438)
                     /locus_tag="PK264_06035"
     CDS             complement(9131..10438)
                     /locus_tag="PK264_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75486.1"
                     /translation="MRFKYIGLAALAVGAGAAAVVAQTPNEQFIPALVYRTGAYAPNG
                     VPFANGTADFFAMLNERDGGVNGVKLVVEECETGYATDRGVECYERLKNKGPTGAAFF
                     MPLSTGITFALTEKAPVDKIPLLTTGYGRSESRNGEVFPWNFPILGTYWTAADIIMQH
                     IAKKEGGWDKLKGKKIALVYHDSPYGKEPIALLSKRAQMHGFEFIPLPVTHPGVEQKA
                     TWLQIRQNRPDYVLLWGWGVMNSTSIKEAVSVGYPRDKMYGVWWSGAEPDVRPAEDGA
                     KGYNAVTLQHTSGRYKIHDDLKKFLYDKGKGVAKWDETSEVLYIRGLINGFLGTEAIR
                     TAHAKFGKKPLKGEEVRWGFENLKLTAERIKEAGFEGVLQPITLSCQDHEGARTGRIQ
                     TWDGKEWKITSDWYVGDDSVLVPMVKETATKYAADKKLTAKCL"
     gene            complement(10546..11613)
                     /locus_tag="PK264_06040"
     CDS             complement(10546..11613)
                     /locus_tag="PK264_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD75487.1"
                     /translation="MLYRETGQFKTRYADDQAIFPIAQDRWFVIGVIAFAFLVIPFIA
                     TQYLYTEVLLPVLILSIAAIGLNILTGYCGQVSLGTGAFMAVGAYAAYNFWLRVPFTT
                     DIVACVVLGGVTAAVFGILFGLPSLRIKGFYLAVATLAAQFFTDWATARVKWLTNYAP
                     SGSVNVSNLKFFGFPVETPGQKYIVVLIFLVVLALIAKNIVRGRIGRMWMAIRDMDIA
                     AEIMGIKPLTAKLSAFAISSFYIGIAGALFAFLRLGAWEPLAFDINRSFQLLFMVIIG
                     GLGSILGSILGAIFIVLMPIFLNQLPIWLGIPLSTATIAHIEFMVFGAMIVIFLILEP
                     HGLARLWAIMKEKLRLWPFPY"
     gene            complement(11627..12549)
                     /locus_tag="PK264_06045"
                     /pseudo
     CDS             complement(11627..12549)
                     /locus_tag="PK264_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            complement(12723..>12875)
                     /locus_tag="PK264_06050"
     CDS             complement(12723..>12875)
                     /locus_tag="PK264_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75488.1"
                     /translation="IALIEHDMGVVMDLSDRVVVLEYGQKIADGTPVEVQANQKVIDA
                     YLGVSH"
     assembly_gap    12876..12904
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12905..13514)
                     /locus_tag="PK264_06055"
     CDS             complement(<12905..13514)
                     /locus_tag="PK264_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD75489.1"
                     /translation="MTVTADGREIGAQLLTVENVSLSFGGVKAIRDVSFDIRKGEIRA
                     IIGPNGAGKTSMLNVINGFYHPQIGTITFKGAKRHEMRPYIAASQGISRTFQNVALFK
                     GMTTLDNIMAGRTLKMRTGLLWQALHIGRARDEEMKNREIVEKIIDFLEIQHIRKTPV
                     GKLPYGLQKRVELGRALAMEPELLLLDEPMAGMNLEEKEDMSR"
     gene            complement(13535..15547)
                     /locus_tag="PK264_06060"
     CDS             complement(13535..15547)
                     /locus_tag="PK264_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75490.1"
                     /translation="MSATSSAAATASKPAVFDTVPKLLLRNAERFGSRPAYRHKDFGI
                     WQTWTWAQALEEIRAFSLGLKALGLGAGEKVAIIGNNRPRLYWAFAAIQSLGAIPVPM
                     YQDGVAEEMAYVLDHADISFAVVEDQEQVDKLVTVGPRLPKLHKVIYDEPRGLTKYPH
                     ENMHAYEDVQRMGRDELKRNPKADADWRAGIAAGKGSDISVILYTSGTTGKSKGVMLS
                     HDNVVISGNNANAFDRFTADDTMIAYLPMAWVGDHIFSYGQAYSGALCVACPESPETA
                     QEDRRELGPTYFFAPPRVFENMLTQIVVRMEDAGAIKRRMFHYFLEHAKKVGPAILDG
                     RSVGFLDKLKYRFGDLLVYGPLRNRMGFSKLRVAYTAGEAIGPELFSFFRALGINLKQ
                     LYGQTEASVFVTLQPNGEVFPETVGKPGPGIEIKVDDAGEVLFKSPGVFVEYYKNEEA
                     TKSTKTPDGWVKTGDAGFIDQRGHLQIIDRAKDVGRLNNGALFAPKYIENRLKFYPEI
                     KEAVAFGHGRDYCACFINIDLVSVGNWAERNNIVYSSYQELSQLPAVYEMIEKRVDEL
                     NRVLAGEPRMFDSQIRRFLILHKELDADDGELTRTLKVRRGFIAERYGPFIKALYDGS
                     KTASISTEVTFEDGRTGRIEGDVRIIDMKAHPAPKSAATPIKEAAE"
     gene            complement(15799..17727)
                     /locus_tag="PK264_06065"
     CDS             complement(15799..17727)
                     /locus_tag="PK264_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HRD75491.1"
                     /translation="MSKPESPHILPYGTWPSPIGPEAVARASRRLGHVVADGACIYWT
                     ESRPEERGRQVIMRAPAMGGVAEAILPPPFSANARVHEYGGGEFIAVDGTVYFVNDGN
                     QDVYCVRPGETPERLTQAPDTRFADFTLDRARRRLICVAERTSADPTRPENLLVAIGL
                     EGSARGAVRPLLQGRDFFANPVMDHEGGRLAWIAWDLPGMPWDEAALYTATIDANGKV
                     GAPRRIAGGDGTAAGEPIWGHDGRLYFISDETGNGAIYCDDGRNVERLTSGRSDLARP
                     MWSLGTRSFAVGRDGRVWWTEMAGAEALILVRRPDDGSIMQRRTGFASLGHMVANADG
                     IGGVAAIAGRPAAPPAVVAFEVKALALSVIREAAAAPIAEDLVSRGQAITFAGGDGAL
                     THAFYYPPRGDRVAGPCGAKPPLLVLAHGGPTGAADAGLKLRIQFYTSRGFAVCDVNY
                     AGSTGFGRRYRERLDGAWGVADVADCAAAARHLAATGRADGARMAIAGGSAGGYTTLM
                     ALATTSDFAAGSCHFGIADLGLLLAHTHKFESGYLHRLLGTTRGNYAETFAARSPINH
                     IENIAAPVIIFQGLDDKVVPPEQSRLMHRTLLAKGIASELHEFAGEGHGFRRAETIAA
                     VLEAELRFLVAALRLDHG"
     gene            complement(17735..19534)
                     /locus_tag="PK264_06070"
     CDS             complement(17735..19534)
                     /locus_tag="PK264_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD75492.1"
                     /translation="MTKLISRRHLSFILDEVIDVARLCRLPRFAEHDRASFSASLDMA
                     EALAIEELLPSYALLDREEPRLVAGRVVLPAATARSINAMRALGLFGMYAAPAVGGLG
                     LPRTIANAALLWLQAANVSIANYLLLTSSAAELLAVHGSIEQKRRYMQPMLEGRFLGT
                     MALSEPQAGSSLGDITTRARLRADGRYALSGSKMWISGGEHDLAETIVHLMLARVEGA
                     PAGVKGISLFIVPRDRLDAEGRPCGANHIAVAGLNHKMGQRGTVNCALEIGDGGETIG
                     EIVGAPGQGLAQMFTMMNEARIGVSLGAVAHASAGFLHALAYARDRRQGRHPDQKDPD
                     APPVAIIEHADVRRMLLDQKAAAEGGIALGLDLAMLADLKAAAETAAERADAGLLLEL
                     LTPVFKAWISDEMLAASSSSMQVLGGAGYTRDHPLEQHYRDNRLNPIHEGTNGIQAID
                     LVGRKLGLGGGRALALLIERIGATISGARSHAALADAASGLAEATRMLAETAARLREI
                     GRTRGSAAMLAPAHHLLDLVGEVVFVWTWLRQAEVAARALPAAEGPDRDFYAGKIAVL
                     GHVARHRLPRIAHLGRLALDPGSYLDLSSDCFD"
     gene            19657..20391
                     /locus_tag="PK264_06075"
     CDS             19657..20391
                     /locus_tag="PK264_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD75493.1"
                     /translation="MPNETVPSVAFVPAAGLGTRMRPLTDKRPKPLVEVAGRALLDHV
                     LDRLAAAGIERAVVNVHYLADQIERHLAGRGRPRIAISDERAQLLDTGGGVAKALPLL
                     GAEPFVVHNSDTIWHEQGTDNLRRLMTFWSPPRMDLAMLLARRDTSLGYDGHGDFELD
                     ADGRIRRRAPGTTATYVFAGVSIMAPSLLADAPPVAFSLNRPWDQAIASGRACAIVLD
                     GTWMHVGDPAALAAAERFLGAAHGRG"
     gene            20378..>20980
                     /locus_tag="PK264_06080"
     CDS             20378..>20980
                     /locus_tag="PK264_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HRD75494.1"
                     /translation="MAAADAVHSEAVFTAPPGVPFLDAVARAILAGDLPRPGGAPPSI
                     EGLADVTILLPTRRAVRHLTEAILVASGRRAVLLPNIRPIGEGDEDLGLLDGLLAPGN
                     ATGFGKDAAHLDLPPAISAIERRLTLTRLVLMWSKALRARAETDARPLSGPSTPAQAA
                     RFADDLGRLIDMLETEGVSPSRLAGLVPDAFAEHWQETVEF"
BASE COUNT         3566 a         6996 c         6758 g         3595 t
ORIGIN      
        1 ggctgacctc gacgagctga agaagaaggg cgtcaacatc gtgcgcacgc ccgacagcgt
       61 gctcgaggcg cagctcaagg cttgggacaa ggtgatcgcg gcgcagtccg cggatgccaa
      121 gaccggcgcc ttcttcgcca aggtgatcgc ctcgcagaag gcctgggtta agcgttccgc
      181 cggctacatg caggcgaaca acgtgccgac cacgatggcc tacaagcact tcttcggctg
      241 atttcagtct tccagcgcga ccgcgcgggc ttcgacgtcc gcgcggtcaa ttgcgtttgt
      301 cggccagcgg gccgcgccgg gggaattcca atgaaccgcc tgctcttcct ggtcgacgca
      361 ctcagctcgt tcgtcggcaa gctcttcgcg tggtgcatcc tgctgctgac gttcgcgatc
      421 tgctacgagg tcgtcgcgcg ctacgctttc ctggcgccga cagactgggc ctacgacgtc
      481 tcctatatcc tatacggcac gctgttcatg atggcgggcg cctacacgct ttcccgcaac
      541 ggacacgtgc gcggcgactt tctctatcgg acctggcagc cgcgtgccca ggcctggtca
      601 gatctcatcc tctacttcgt tttctacttt cccggcattc tcgccctgat ttatgccggt
      661 tggaaatact ttcacctctc ctacctgatg aacgagcatt cctcggcctc gcccaacggc
      721 ccggtcattt ggcccttcaa gggcgtaatt ccgttgacgg gaattctcct gctcctgcag
      781 ggcctcgtcg aggtcgcgcg ctgcatcatc tgcatccgga ccggcgagtg gccgcagcgc
      841 ctccatgacg tcgaggagct cgaaaaggtg attctggaga aagcccaagc cgaccaggct
      901 gccgctcccg gagccaagcc atgaccctcg accagctgct acgctccgtc atctcaccgc
      961 tcatctccga tccggcgatg ggcatcctga tgctcctgct gttcctcgtc ttcctgatgc
     1021 tcggattccc gatcgccttc acgctgatgg cgatgggcat cggcttcggc tactacgcgc
     1081 gcggcgacag cgtcttccag ctgttcgtcc agaagacatt cggcgtgatg tcatcggacg
     1141 tgctgctgtc gatcccgctg ttcctgttca tgggctacat ggttgagcgg gccaacatcc
     1201 tggaccggct gttccgctcg atccagctcg ccgccggctg gctgccggga tcgctcgccg
     1261 tcgcgacgct cgtcacctgc gcactctttg cgaccgcgac cggcatcgtc ggcgccgtcg
     1321 tgacgctgat gggcctcctc gcctttcctg ccatgttgcg cgccggctac gatacgcggc
     1381 tggcggcggg cgtcgtctgt gcgggtggct gcctcggcat cctcattccg ccaagcgtga
     1441 tgctgatcct ctatggcgcg acggcaggcg tctcggtcgt caaactctac gcggcagcct
     1501 tcatcccggg cgtgatgctg gccggactct atattctata cgtcgtcgtg ctcgcgatgc
     1561 tgaagcccga gctcgctccc aagctgccgc cgcaggagcg caatgcaccc atcggtcgcg
     1621 tcatctggga actcgccacg tcgttcttcc cgctggcact cctcattctc ggtgtgctcg
     1681 gggcgatcat gttcggcttc gcctcgccgc aggaggctgc agcagtcggc gcgctcggtg
     1741 cgctccttct cgccatcgcc taccgcagct tctcctttga gaagctcaag gagagcgtct
     1801 atctcacggc gcgcgcgtcg gcgatggtgt gctggctgtt cgtcggctcg gccgtatttt
     1861 cggccgtgtt cgcccagctc ggcggacaaa aggtcgtcga ggatttcgtc ctctccctga
     1921 acctctcgcc cttctggttc ctgatcatga cgcaggtaat catcttcatc ctcggctggc
     1981 cgctcgagtg gacggagatc atcataattt tcctgccgat cttcctgccg ctgctcgacg
     2041 acttctgcat cgacccgatg ttgttcggag tgctcgtcgc gctcaatctg cagacgtctt
     2101 tcctctcgcc gcctgtcgcg atggcgccct tctacctcaa aggcgtggcg ccgaagcacg
     2161 tgacgatcaa ccagatcttc aagggcgtgt accccttcct ctacattgtc gtgctgtcga
     2221 tggtgctctt ctacgctttc ccgcagctcg gcacctacct tcccgagaag ctctacagcc
     2281 atcgcccgcc gaaatgcgcg atccagccca agaaggccga ggctcaacca cccatcgaag
     2341 gcgccgcgct cgcccagccg ggcgcgaccg tcgccagctg cccctggcgc agctcctaca
     2401 agcccgtgtg aaagggcaac catggccaag caatcgttca tcgacctttc ggcaaccgag
     2461 gcctcggcgc tgctctcacg cggggaggtc tccgccgtcg agtacgtcac ggcctgcctc
     2521 gagcgcatcg ccgagcgcga agaggaggtg cgcgccttcg agcatctcga cgccgaacat
     2581 gcgctccgcc aagcgaaggc cgccgacgag gcccgcgcga tcggcctcgg cgtcggcccg
     2641 ctcaacggcc tgccggtcgg tctgaaggac atcatcgaga ccgccgacat gccgacgggt
     2701 aacggctcgc cgatcttcaa gggctaccag ccgacgcggg atgccgtctg tgtcagccag
     2761 ctcaagaccg ccggcgcggt catcatgggc aagaccgtga cgacggagct tgcgacgctc
     2821 gttcccggaa agacgcgcaa tccgcacaac ctcgcacaca cgccgggcgg ctcctcttcg
     2881 ggctcggcgg ccgccgttgc cgatcgcatg atcccgctgg ccctcggcac ccagaccggc
     2941 ggctccgtga tccggcccgc ctcgttctgt ggcatctttg gcctgaaacc gacgctcggc
     3001 ctcatctcgc ggcgcggcgt caccatgcaa tcacacaccc tcgacaccgt cggcgtctat
     3061 ggccgctccg tcgaggacct cggcctcgtc gcggacgcgc tctcggcgca cgatccagac
     3121 gacgaggtga gctacccgcg ggcgcggcca tcgctcgcgg caacgcttgc cgaggatcca
     3181 ccggttccgc ccctcctcgc cttctgcaag acgcatgtct gggatcaggc cgaggccgcg
     3241 accaaggacg cgttcgccga gttggtggcg accctcggca atcgcgtgca ggaggtcgac
     3301 atcccggcgc tcgagcttgc gcgcaaggcg cagcccgtcg tgcagggcac ggagaatgcg
     3361 gcctactatg gcccgctcct cgacaagtat ccggaaggcg tggcacccgc gctcgccgac
     3421 cgcatccgcg ccggccgcaa gttctcgggt gaggagtacg tgcgcgcgct ccgcgaccgc
     3481 gagacgatct atgccgctgt cgagcggact ctcgtcgact tcaccgcaat cctgacgcca
     3541 gcctcctgcg ggcctgcacc caagggcctc aaatcaaccg gcaacccggt cttcaacgcc
     3601 acctggacgc tgctcggcgt gccgtgtgtg acgctgccgc ttctcgatgt cgacggcctt
     3661 ccgcttggcg tgcaactcgt cggccgccgc cgcgacgatg gccgcctgct ccgtaccgcc
     3721 cgctggctcg cgcgcgagct ggcgcccaag ccgaggaagc gcaagtcgaa agcgtgaggt
     3781 tgaccactca ggcagcggtc agcgcaccat aaagcagcgt cagtcctgcg agcgccatca
     3841 tggcatcgat cacgaagcgg aactggcttg cgtccatgcg ctcgacgagg cgcttggcaa
     3901 tccacgagcc gatccagagc gaggcgccaa ccgcgcatcc cttgctgatc gtctccacgg
     3961 ggagcgagcc aaacgagcgg aacgtgattg ccttggtgat gccgatcaac gccgagccga
     4021 gcgcttcggt gccgaggaag gcgcccttgg tgaggccgta ggcgagaaag aacggcgtgt
     4081 tgagcggtcc cgtcgaggcg acgatgccgg tcaggaaccc tattcccgcg ccgaccacgg
     4141 ctagatgcgg cagtccgacc ttgaacccct tggcgagcag ccagcgccgc actgggatca
     4201 tgacgatgaa gaagacgccg aggatcgtct cgaccatgcg ggtgtcgagc gccagcagtg
     4261 tccgcgcgcc gaaatacgcg gccggcagcg ccgtcgcgca gtagaccacg ttaaggcgga
     4321 cgtctacatc ccgccaccac acgaccacgc gcgagacatt ggcgatgata ccggcgatcg
     4381 ccatgatctg cacggcctcc ttggcgccga acccgaggac gaggatcggc atcagcatca
     4441 cggaggcgcc gaagccgacg atgccgccga gcgtgcccgc cgcaatgccg atgacgagga
     4501 ttgcggccca gatcatgcgc gctcaggccg acagcgcttc gacgacgaga ttgcggttcg
     4561 tgtagacgca gatgtccgcg gcgataccca tggccttcct tgcgatcgcc tcggcatcga
     4621 gcggctgatc gatcagcgcg cgggccgccg cgagcgcgta cattccgccc gatccgatgc
     4681 ccatcacgtc aaactctggc tcgagcacgt cgccagtgcc ggtgaggacg agcgtcttgc
     4741 cgcgatcggc gacgatcatc atcgcctcga gccggcgcag gaagcggtcc gtccgccagt
     4801 ccttggcgag ttccactgcg gcgcggacca gctggcccgg atgctgctcg agcttggctt
     4861 cgagccgctc gaacagcgtg aacgcgtcgg ccgtggaacc cgcgaagccg ccgatgacgt
     4921 cgccgcgtcc gagcgagcgc accttcttgg cgttggcctt gatgaccgtc gcgccgaggc
     4981 tcacctgccc gtcgcccgcg atcaccacgc ggccgccctt gcgcacggtc aggatggtcg
     5041 tgccgtgcca ttgcgtctgg cctgcatcgc ccgacatggt tgccgacatg ggacgtcacc
     5101 ttctcctaaa ctctgccata cagccaaggt ggcacgcagg gcgcgaaact gctatgcccg
     5161 cggcctaacg cgcatgcaag agcagagcgg gagacgcgcc gtggcacgcc aggcaacaat
     5221 cgagcgcagc accaaggaga cgtcgatcaa ggtcaccgtc gatctcgacg gcaccggccg
     5281 ctcgcggatc gcgaccggca tcggcttcct cgaccacatg ctcgagcagg tcgcgcgcca
     5341 cggcctcatt gacatcgacc tcgaggccaa gggcgacctg cacatcgact tccaccatac
     5401 caccgaggac agcggcatcg ccctcggcca ggcgatcgcc aaggcgctag gggcgaagca
     5461 gggcatcacg cgctatgcgt ccgcctacct ccccatggac gaggcgctga cccgcgtcgc
     5521 catcgacgtc tcgggccggc cgttcctcgt ctgggacgtg acgttctcgc ggccgaagat
     5581 cggcgagatg gatacggaac tcttccgtga atggttccag gccttcgcga tgaatgccgg
     5641 catcacgctt cacgtcgcga cactctacgg cgacaacaac caccacatcg ccgagagctg
     5701 cttcaagggc cttgcccgct cgctcaagga cgcgatcgcc atcgatccga ggcaggccgg
     5761 gcgcgtgccc tcgacgaagg gtacgctcac cggctgagcg aggccggcga cgtcgtccga
     5821 cgtctgcgga gccaatttgt aagatcagga cccgaccaaa ctccagctgc ggggggaggg
     5881 gggatgagct ggcgttggtc gggtccctgc ttccgggtgc ggggcccgga ccgtccggct
     5941 cgcctcgcgt cgatgctgca tcggcagcat ttgggcgcaa ggccagcgca acttgcagtg
     6001 ccgcgctgag cgcacggcat atccgtcaat gatcaggccg cggcacccac acaggagcgg
     6061 tactgaccgg tggcggaggc ggaaggaagg agccaaggct tgttggtgct gcaggcagcg
     6121 gcgccgcagg ctggcccggg gcgaccggcg agctcgcctc ggattccatc gggccgccaa
     6181 gcgcgctcgc tccggccggg cgtggcgcct ttgcgacggc ctttggtgaa acaggagcgg
     6241 cggccacaga aggcgcgggt gccgcagcag ggggaagggt cggcgctgcg ccgtcctttg
     6301 cgattggacg cggctcatgc acccgcgccg acgacatgat gggaactggc acggccgaca
     6361 cgtccgactt cggctgtgcg ccgcccttgg tcgcgatcgt cagcggcggg gtcgcatcgg
     6421 tgacatcggc ggcgggcggc acgacggcat cggcggtctt gctcggcttg cgcttggcgc
     6481 ctgacgacgt cggaggctgg accggcgccg ctgcgaccat ctcgacagtc ttgaggaggg
     6541 cgcgggcgcg ggcaagccgc gtgccgtgct cgtcgatggc gtccaccgtc agctcgaggt
     6601 gcttgacgtc atcgggaagc gagacgcgga gtgcgccgag gtccgagctc gcgacgatcc
     6661 aggcgccggt ctgggttggc ttgccgtgcg acaaggtcac gccggccggc aggttgctga
     6721 tcaggatcga cacggcccgc ggctcgatac cgtcgtgcca cttgaggtcg agcggaatgg
     6781 gcgccgcccg acgatcctcc gcgcgaacgt gcagctcgaa gccgatgcgg acacgaccgt
     6841 gatccgacga cgcgtgggca tggtcgggat cgaagaacac cgcgtgcggg cgctcctcgg
     6901 cgatcacttg ccagtgatgg gccgccctgt cgccgtcgac gcctgtcctt tccggtgccg
     6961 ccgccttcgc gaccgtcgac tttgcgacag gcttatttga ggaggtgcca gcgagcgtgt
     7021 cgagcggccc gccgacctgc acgaggtagg caagcccgcc gaatgccacc gcggcgccga
     7081 caccggcaaa agcccacagc cgaccgccgc ggcgcgatgc cgggacagct tcggcgccac
     7141 gcgcgctggc gatcgccgaa tgcaactcgt cgaacgaggt cccctgacct ctgagcgcga
     7201 gccgctcatc ggcgtcaaag ctcggtgcgg ccgtggcatt cggcacgcgg ccgatctgga
     7261 gcccggcctt cggcggtggc ttgatgccga agagctgggt cgtcactgcg tcgagcgccg
     7321 cctcgatcgg gtcggctgac gggcgatgta cctcgctgtg tgtggcgggt tgcgtcggca
     7381 ggatgcctcg cggcagcggc tcggccagca ttggcgcggg cgccggaatg gttgcaagct
     7441 cgcggtcgac tgggcgcggc agcgaattgc cgggaaccgg gcggtgcgct gcatccgtgg
     7501 cgactggccg gtgagccggt ggtggcagcg cggcttgcgc atggcgcgcg tctcgatagc
     7561 ccgcttcgcg ttggccgacc actggcgttg cggctggcgg aagcatcgga gcattctcga
     7621 cgatccgcaa ggtttcctcg aggatgcgct cgaaggatgc gtcggccggt ggtgtgggga
     7681 atgccagacg tcccgccgga gcggcacgtg ccgccagggg cgccggtggc ggcaaggcgg
     7741 cattccgctc aatgctgtcc aggcgctcga tcaggccttg gcgccagatg tcgtgcacat
     7801 cgcgtggatc gttcgaatgc atccgcgcct cctcgacgca acccggtcgc gccgcggcgg
     7861 atgagcggct cgaaccattg ggcaacggcg cgtctgccaa gctggcagga gcggtcgccc
     7921 cctctactgt ccgcgaagga aacacagagg atttggcgcc ggaagccaaa agcacagccc
     7981 aaggacaata atcggctttt caacagcaaa tttccacctg acggaagaat agcgtggaga
     8041 agcaattcaa atctgtggat ataatcaaat ccgccacttg acgcggcatg gcgaaaaacg
     8101 cggcagcgat cttcgttcaa gcctcgacct tatcggccgg acgatgagac ggtgatgaac
     8161 agcatcggcc tacgcgccgg gggcaccaaa aaaatggccg gcacaggggc cggcctcgat
     8221 cgatcaatcc tgggacgccg tcaagcgagc cagcgcttgc gccgcttgta gtgcttcaca
     8281 tcccggaacg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag gtagaactcc
     8341 ttcacgtcgg cgttctcgcg caaggcctcc gccgtgccgt ccatgacgac gcggccattc
     8401 tcgaggatgt agccgtaggt cgcgtagcgc agcgccatgt tggtgttctg ctcggcgagc
     8461 agcaccgaca ctccctgctc ctgattgatg cgcttcgtga tctcgaagat ctgctcgacg
     8521 agctgcggcg cgaggcccat cgagggctca tccaacagta ccatcttcgg ccgcgacatc
     8581 agtgcccggc cgatcgccgt catctgctgt tcgccgcccg agatgtagcc cgtgaggctc
     8641 gtccggcgct ccttcaagcg cgggaagtaa ttgtagatca tctcgaggtc ggctttcacc
     8701 gcgccccgcc cgtcacgacg tgtgtaggcg ccggtcagca gattttcctc gacattcaga
     8761 tgagcgaagc agtgccgccc ctccatcacc tggatgcagc ccttcttgac gagcgcattc
     8821 ggcgactcgc ggtgcacctc ctcgccgtcg aatacgatcg agcccttggt gacctcgccg
     8881 cgctcgccgt gcagcaggtt cgagaccgcg cgcagcgtcg tcgtcttgcc ggcgccgttg
     8941 gcgccgagga tcgccacgat cgcgcccttc gggacctgaa gcgacacgcc cttcaggacc
     9001 agaacgacgt gattgtagat cacctcgatg ttgttgaccg acaggatcgg ctggttctcc
     9061 gccgccgtcg cgggagacga cggccgctga gcggccgccg tccctgtcgt tgctggtgcc
     9121 gctgtcgaca tcagaggcac tttgccgtga gcttcttgtc ggccgcatac ttcgtggcgg
     9181 tctccttgac catcggcacc agaacggagt catcgccgac ataccagtcg gaggtgatct
     9241 tccactcctt gccatcccac gtctggatac ggccggtacg ggcaccctcg tgatcctggc
     9301 aggacagtgt gatcggctgg aggacgccct cgaagccggc ttccttgatg cgctcggccg
     9361 tcagcttaag gttctcgaag ccccaacgga cctcctcgcc cttcagcggc ttcttgccga
     9421 acttcgcatg cgccgtacgg atcgcctcgg tgccgaggaa gccgttgatc aagccgcgga
     9481 tataaaggac ctccgaggtt tcatcccact tcgcgacgcc cttacccttg tcgtagagaa
     9541 acttcttcaa gtcgtcgtgg atcttgtagc ggccagacgt gtgttgaagc gtgacggcgt
     9601 tgtagccctt agcgccgtcc tcggcgggac gtacgtcagg ctcggcgccc gaccaccaca
     9661 cgccatacat cttgtcgcgc gggtagccaa ccgagacggc ttccttgatg gaggtcgagt
     9721 tcatgacgcc ccagccccag agcagcacgt agtcgggacg gttctggcgg atctgcagcc
     9781 aggtcgcctt ctgctcgacg cccggatgcg tgacgggcag cggaatgaac tcgaagccgt
     9841 gcatctgggc acgcttcgat aggagagcga tcggctcctt gccatacggg ctgtcgtgat
     9901 agacgagcgc gattttcttg cccttgagct tgtcccagcc gccctccttc ttggcgatgt
     9961 gctgcataat gatgtcagca gccgtccaat aagtgccgag gattgggaag ttccagggga
    10021 acacttcgcc gttgcggctc tcagagcggc catagccggt ggtgagcagc gggatcttgt
    10081 cgaccggcgc cttctcggtg agagcaaagg tgatgcccgt cgacagcggc ataaagaacg
    10141 cggcgcccgt tggacccttg ttcttcagac gctcatagca ctcgacgccg cggtcggtgg
    10201 cgtagcccgt ctcgcactcc tcgacgacga gcttgacgcc attgacgccg ccgtcgcgct
    10261 cgttgagcat ggcgaagaag tctgcggtgc cgttggcgaa cggcacgccg ttcggcgcgt
    10321 aggcgccggt gcggtagacg agcgccggga tgaactgctc gttcggggtc tgcgcgacga
    10381 cggcagcggc acctgcgccg actgccaggg ccgctagccc tatgtacttg aagcgcattc
    10441 gtttcctcca cttgacgccc gggggttccc ccggagctga actcattttc tccccctggc
    10501 aaggcgaacg gaagcccgac caaggttgcc tcgaccgaaa gtccttcaat acgggaacgg
    10561 ccagaggcgg agcttctcct tcatgatcgc ccagagccgg gcaagcccat gcggctcgag
    10621 gatcaggaag atgacaatca tggcgccgaa caccatgaac tcgatgtgcg cgatggtcgc
    10681 cgtcgagagc gggatgccga gccagatcgg cagctgattg aggaagatcg gcatcagcac
    10741 gatgaagatc gcgccgagaa tcgagccgag gatcgagccg agcccgccga tgatgaccat
    10801 gaacagcagc tggaacgagc ggttgatgtc gaaggccagc ggctcccagg cgccgaggcg
    10861 caggaaggcg aacagcgcgc cggcgatgcc gatgtagaag gaggagatcg cgaaggcgga
    10921 gagttttgcc gtgagcggct ttatgcccat gatctcggcg gcgatgtcca tgtcgcgaat
    10981 cgccatccac atgcgcccga tacgacctcg cacgatattc ttggcgatca acgccagcac
    11041 cacgaggaag atcagcacga cgatgtactt ctggcccggc gtctcgaccg gaaagccgaa
    11101 gaacttgagg ttcgagacgt tgacagagcc ggacggcgca tagttcgtga gccacttgac
    11161 gcgcgccgtc gcccagtcgg tgaagaactg cgcggcgagc gttgcgactg cgaggtagaa
    11221 gcccttgatg cgcagcgacg gcaagccgaa caggatgccg aacacggcag ccgtcacgcc
    11281 gccgagcacg acgcaggcga cgatgtccgt cgtgaacggc acgcgcagcc agaaattgta
    11341 ggcggcgtag gcgccgaccg ccatgaaggc accggtgccg agcgagacct ggccgcagta
    11401 gccggtgaga atgttgaggc cgattgcggc gatcgacagg atcagcaccg gcagcagcac
    11461 ctcagtatag aggtactgcg tggcgatgaa cgggatcacc aggaaggcaa aggcgatcac
    11521 gccgatcacg aaccagcgat cctgggcgat cgggaagatc gcctgatcat cggcgtagcg
    11581 tgtcttgaac tgaccagtct cgcggtagag cacggaagcc tgcctctcag attcgctcga
    11641 tgatcttttc gccgaagagg ccctgcgggc gcacgagcag cacgcacaac gcgagtacgt
    11701 aggcgaacca gttttcgatg ccgccgccgg cgagatcgaa gaacttgatg agcggcgggc
    11761 cgagcatgac ctcggcgagc ttctccccgg cgccgatgat aagtccgccg atgatcgctc
    11821 ccgggatcga cgtgaagccg ccgatgatga gcaccggcag cgccttcagc gcaacgagcg
    11881 agagcgagaa ctgaaccccg agcttggcgc cccagatcat gccggccacc atcgcaacga
    11941 cgcccgcgac cgcccacacg agcagccaga tggtgttcaa cgggatgccg acggactggg
    12001 cggccggtcg tcggcgacag cacggagcgc acggccgacc cgcgtatact ggaagaagaa
    12061 gaacagcgcg atgaccagca cgaagcagat gatgccggcg accacatcga gccgatcgat
    12121 cagaatgccg ccctggaaca agctttccag cgcgaacatc ggatccttcg agatgccgag
    12181 gttgatcttg tagatgtcgg agccccagat cgtctggccg atgccgtcca ggaagtaggt
    12241 gacgccgatc gtcgccatga acaggatgat gccctcctgg ttcacgagct ttctcagcac
    12301 gaggtactcg atcgcgtagg cgatggcgac catgatggcg accgtcagtg cgattgccag
    12361 tacgatgtgc atcttctcgg agagtcgcgc gatcgaaagt gctgcaaaca gcaccatcgc
    12421 gccctgcgcg aagttgaata cgcccgaggc cttgaagatt agcacgatgc cgagggcgac
    12481 gagcgagtag agcacgcccg tcagcagtcc ggcgagcagc gtctcgagga agttgcccgg
    12541 attctcgatc gccttcgcga cgagaaagcc tatgaagagc gcgacgatca ccgtcagcac
    12601 cagatggaca ccgtgatcga cgccggtctt cctatcgcga cgcagcgccc aatagaagag
    12661 cgcggcaaac gcggcaccga tgccgcccgg aagcgctaag gctagacccg acatggaagc
    12721 tcctagtgcg aaacgccgag gtaggcgtcg atcactttct ggttggcctg aacctcgacc
    12781 ggcgtgccgt cggcgatctt ctggccgtat tcgaggacga cgacgcggtc cgagaggtcc
    12841 atgacgacgc ccatgtcgtg ctcgatgagc gcgatnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnaacgcg acatgtcctc cttctcctcg aggttcatgc cggccatcgg ctcgtcgagg
    12961 aggaggagct ccggctccat ggcgagcgcg cgaccgagct ccacgcgctt ctggaggccg
    13021 tagggcaact tgccgacggg cgtcttgcgg atgtgctgga tctcgaggaa atcgatgatc
    13081 ttctcgacga tttcgcggtt cttcatttcc tcgtcacggg cgcggccgat gtgcagcgcc
    13141 tgccagagga ggcccgttct catcttgagc gtgcggcccg ccatgatgtt gtcgagcgtc
    13201 gtcatcccct tgaacaacgc gacgttctgg aaggtgcgcg agatgccctg gctcgcggcg
    13261 atgtagggcc gcatctcgtg gcgcttggcg cccttgaagg tgatcgtgcc gatttgcgga
    13321 tgatagaaac cgttgatgac gttcagcatc gacgtcttgc cggcgccgtt cgggccgatg
    13381 atggcgcgga tctcgccctt gcggatgtcg aaggagacgt cgcggatcgc cttcacgccg
    13441 ccgaacgaca gcgagacgtt ttctactgtc aggagctgcg ccccgatctc gcggccgtcg
    13501 gcggtgacgg tcatactcgg attgacgtcg gccttcattc cgcggcctcc ttgatcgggg
    13561 tcgccgctga cttgggcgct ggatgggcct tcatgtcaat gatccgcacg tcgccctcga
    13621 tgcggcccgt gcgaccgtcc tcgaaggtga cctcggtcga gatgctcgcc gtcttggagc
    13681 cgtcgtagag cgccttgatg aagggcccgt accgctcggc gatgaagccg cgccgcacct
    13741 tcagcgtccg cgtgagctcg ccatcatccg cgtcgagctc cttgtgcagg atcaggaagc
    13801 ggcggatctg actgtcgaac atgcgcggct cgccggcgag cacccggttg agctcgtcga
    13861 cgcgcttctc gatcatctca tagaccgccg ggagctgcga gagctcctgg tagctcgagt
    13921 atacgatgtt gttgcgctcg gcccagttgc cgacggagac gaggtcgatg ttgatgaagc
    13981 aggcgcagta atcgcggccg tgtccgaagg cgaccgcctc cttgatttcg ggatagaact
    14041 tcagccggtt ctcgatgtac ttcggcgcga agagggcgcc gttgttgagg cgtcccacgt
    14101 ccttggcgcg atcgatgatc tgcaggtgac cgcgctgatc aataaaaccg gcgtcgcccg
    14161 tcttgaccca gccgtcgggc gtcttggtcg acttcgtcgc ttcctcgttc ttgtagtact
    14221 cgacgaacac gccgggactc ttgaagagca cctcgcccgc atcgtcgacc ttgatctcga
    14281 tgccgggacc gggcttgccg accgtctccg ggaagacctc gccgttcggc tgcaaggtga
    14341 cgaacacgct cgcctcggtc tgcccgtaga gctgcttcaa gttgatgccg agcgcgcgga
    14401 aaaacgagaa gagttcgggg ccgatcgcct cgcccgccgt gtaggcgacg cggagcttcg
    14461 aaaagcccat acgattgcgc agcgggccgt aaacgaggag gtcgccgaag cgatacttga
    14521 gcttgtcgag gaaaccgacg gacctgccgt ccaggatcgc cggcccgacc tttttggcgt
    14581 gctcgagaaa atagtggaac atcctccgct tgatcgcgcc ggcatcttcc attcgtacga
    14641 cgatttgcgt cagcatgttc tcgaacacgc gcggcggtgc gaagaagtag gtgggcccaa
    14701 gttcgcgccg gtcttcctgg gcggtctcgg gactttcagg gcaggcgacg cacagggctc
    14761 cggagtaggc ctgtccatag gagaagatgt ggtcgccgac ccaggccatc ggcaggtagg
    14821 cgatcatcgt gtcgtctgcg gtgaagcgat cgaaggcgtt ggcgttgttc ccggaaatga
    14881 cgacgttgtc gtgcgagagc atcacgccct tcgacttgcc cgttgtcccc gaggtgtaga
    14941 ggatgacgct gatgtccgag cctttgccgg ccgctatgcc agcgcgccag tcggcgtcgg
    15001 ccttgggatt gcgcttgagc tcgtcacggc ccatgcgctg cacgtcctcg taggcgtgca
    15061 tgttttcgtg cgggtatttg gtgagcccgc gcggctcgtc gtagattacc ttgtggagct
    15121 tggggagccg cggtccgacg gtgacgagct tgtcgacctg ctcctgatcc tcgacgacgg
    15181 cgaacgagat gtcggcgtga tcgaggacat aagccatctc ctcggcgaca ccgtcctgat
    15241 acatcggcac cggaatcgcg ccgaggctct ggatggcagc gaacgcccaa tagagccgcg
    15301 gccggttgtt gccgatgatg gcaaccttct caccggcgcc aagcccgagt gccttgagcc
    15361 cgagcgagaa ggctctgatc tcctcgagcg cttgcgccca ggtccaggtt tgccagatgc
    15421 cgaaatcctt gtgccggtag gcgggccgcg agccgaaacg ctccgcgttg cgcagcagaa
    15481 gcttcggaac agtgtcgaat acagcgggct tgctggctgt cgcagcagcc gaactcgttg
    15541 cgctcacgaa ccacccatcc tcactgcggc ctcaggcgag gcaatcctac cctacagccc
    15601 tacaacggct ttcgccgttg cctgccgatt tgctcggacg ttcttcgtag cgggtaccgc
    15661 cagtactgat cgcggcacgt cgcaccccgg ccgcctctcg ctaggagacg gcagagtaga
    15721 gaggattctc taaggcagcg ctcggattgc cgcaagccgt ccaaaggagg ggtgcggcac
    15781 cacttcacga taagcttatc agccgtgatc gagccgcaat gccgcaacga ggaagcgaag
    15841 ctccgcttcc aatacggctg caatcgtttc cgcccggcgg aacccgtgcc cttcgccggc
    15901 gaactcgtga agctccgagg caatcccctt cgccaggagc gtccggtgca tcaggcgtga
    15961 ctgctcgggc ggcacgacct tgtcgtcgag gccttggaaa atgatcaccg gcgcagcgat
    16021 gttctcgata tggttgatcg gcgagcgggc ggcgaaagtc tcggcgtagt tgcctcgtgt
    16081 cgtccccagc aggcggtgca gatagccgct ctcgaacttg tgggtgtgcg cgagcagcag
    16141 cccgagatcg gcgatgccga agtgacagct ccccgccgcg aagtccgatg tcgtcgccag
    16201 cgccatgagc gttgtgtacc cgcccgccga cccgccggcg atcgccatgc gggcgccatc
    16261 ggcccttccc gtcgccgcca aatggcgggc ggccgcggcg caatcggcga cgtcagcgac
    16321 accccaggcg ccgtcgagcc gctcgcgata gcgccgcccg aagccggtcg agcccgcata
    16381 gttgacgtcg caaaccgcaa atccgcggct cgtatagaac tggatgcgca acttgaggcc
    16441 ggcatccgcc gcgccggtcg ggccgccatg ggcgagtacc agcagcggcg gcttggcccc
    16501 gcacggtccc gcgacacgat caccccgcgg cggatagtag aaggcgtgcg tcagcgcgcc
    16561 atcaccgccg gcaaacgtga tcgcctgacc gcgcgagacg aggtcctcgg caatcggcgc
    16621 agccgcagcc tcgcggatca cgctcagcgc aagtgccttc acttcgaatg ccacgacagc
    16681 cggtggcgcg gcgggccggc ccgcaatcgc cgccacgccc cctattccat cggcattcgc
    16741 gaccatgtgg ccgaggctcg cgaaacctgt ccgccgctgc atgatgctgc cgtcatccgg
    16801 gcgccggacc aggatgagcg cctcggcccc tgccatctcc gtccaccata ccctgccgtc
    16861 acggccgacg gcaaaggacc gtgtgccgag tgaccacatg ggtcgcgcga gatcggatcg
    16921 gccgctggtc agccgctcga cgttccgacc gtcgtcgcaa tagatcgcgc cgttccccgt
    16981 ctcgtccgag atgaagtaga gccgcccgtc gtgcccccag atcggctcgc ccgcagccgt
    17041 gccgtcgccg cccgcgatgc gccgcggcgc gccgaccttg ccattcgcat cgatcgtcgc
    17101 cgtgtagagt gccgcctcgt cccacggcat gccgggcagg tcccaggcga tccaggcgag
    17161 ccggccgccc tcgtgatcca tgaccgggtt ggcgaaaaaa tcgcgccctt gcaagagtgg
    17221 ccgcaccgcc ccgcgcgcgg acccttcgag gccgatcgcg acgaggaggt tctcggggcg
    17281 cgtcgggtcg gcgctggtgc gctcggcaac acagatgagg cgccgacgcg ctcgatcgag
    17341 ggtgaaatcg gcaaagcgcg tatccggcgc ctgggtgagg cgctccggcg tctccccggg
    17401 acgcacacaa tagacgtcct ggttgccatc attgacgaaa tagaccgtac catccacggc
    17461 gatgaactcg ccgccaccat actcgtgcac gcgagcattg gccgagaacg gcggcggcag
    17521 gatggcctcc gccacgccgc ccattgccgg cgcccgcatg atcacctgcc ggccgcgctc
    17581 ctcgggccgg gattcggtcc agtagatgca agcgccgtcc gccacgacat gaccgagccg
    17641 gcggctcgcg cgggcgacag cctccgggcc gatcggggac ggccaagtgc catacggtag
    17701 aatgtgtggg ctctcgggct tgctcatgat gtgttcaatc gaagcagtcg ctggacagat
    17761 caagatagct gcccggatcg agcgccagcc ggccaagatg tgcaatccgc ggcaaccgat
    17821 gccgcgcgac atgcccgagc acggcgattt tgcccgcgta gaagtcgcga tccgggcctt
    17881 cagccgcggg gagcgctcgc gcggcgacct ctgcctgcct gagccaagtc caaacgaata
    17941 ccacctcgcc gacaaggtcg agcaagtgat gcgctggagc cagcatggcg gcgctgcccc
    18001 gcgtccggcc gatctcgcgg agtcgagctg cggtctcggc cagcatccgc gtcgcctcgg
    18061 cgagacccga tgcagcgtct gcgagcgctg catgcgaacg ggcccccgaa atggtcgcgc
    18121 cgatccgctc gatgagtagc gcaagcgccc gccccccgcc gaggcctagc tttcggccca
    18181 cgaggtcgat cgcctggatg ccgttggtcc cctcgtggat cggattgagc cgattgtcgc
    18241 ggtagtgctg ctcgagcgga tggtcgcgtg tgtagccggc gccgccgagc acctgcatcg
    18301 acgagctcga cgcggcgagc atctcgtcgc tgatccaggc cttgaagact ggggtcagca
    18361 gctcgagcag caggcctgcg tcggcgcgct ccgcggccgt ctccgccgca gccttgagat
    18421 cagccagcat tgcgagatcg agaccgagcg cgatcccgcc ctccgccgca gccttctggt
    18481 ccagcagcat gcggcgcacg tccgcatgct cgatgatcgc gactggcggc gcgtccgggt
    18541 ccttctgatc cggatggcgc ccctgccggc gatcgcgggc ataggcgagc gcgtgcagaa
    18601 acccggcgct cgcatgcgcc actgcgccga gcgaaacgcc gatgcgcgcc tcgttcatca
    18661 tcgtgaacat ttgcgcgagg ccctgccccg gcgcgccgac gatctcgccg atggtctcgc
    18721 cgccgtcgcc gatctcgagc gcgcaattga cagtcccgcg ctgccccatc ttgtgattga
    18781 ggccggcgac ggcgatgtga ttggcgccgc acggacggcc ttcggcgtcg aggcgatcgc
    18841 gcggcacgat gaacagcgag atgcccttga cgccggctgg cgccccctcc acgcgcgcga
    18901 gcatcaggtg gacgatggtc tcggcgagat catgctcgcc gcccgagatc cacatcttcg
    18961 atccggagag cgcatagcgt ccgtccgccc tgagccgcgc ccgcgtcgtg atatcaccga
    19021 gcgaggagcc ggcttgcggc tccgagagcg ccattgtgcc gaggaaccgg ccctcgagca
    19081 tgggctgcat gtagcgacgc ttctgctcga tcgatccatg tacggcgagg agctcggccg
    19141 cgctcgacgt cagcagcagg tagttggcaa tcgagacgtt ggccgcctgt agccagagaa
    19201 gcgcggcgtt ggcaatcgtg cgcggcagcc cgagcccgcc cacggctggt gcagcataca
    19261 tgccgaagag gccgagcgcg cgcatggcgt tgatggaacg tgccgtcgcg gccggcagca
    19321 cgactcgccc agcgacgagc cgcggctcct cccggtcgag cagcgcatag gacggcagca
    19381 gttcctcgat ggccaaagcc tcggccatgt cgaggctcgc cgaaaacgag gcccgatcgt
    19441 gctcggcgaa gcgcggcaga cggcagagcc tggccacgtc gatgacctcg tcgagtatga
    19501 aggagaggtg ccggcgcgag atgagcttgg tcatggcgga atgatgcccg aagcgggggc
    19561 tgttcggaag tgggtcgccc gccttcgcta tcgcgggacg atcggctatc aggggagaga
    19621 agatcatcgc atgaggcgaa tggcgggagg agaagcgtgc cgaacgagac cgtcccgagc
    19681 gtcgcgttcg tcccggccgc cggcctcggg acgcgcatgc gaccgctcac cgacaagcgc
    19741 ccgaagccgc tcgtcgaggt cgccggacgc gcgctgctgg accatgtgct cgaccgcttg
    19801 gcggcggccg gcatcgagcg cgccgtcgtc aacgtgcact acctcgccga ccagatcgag
    19861 cgtcatcttg ccggccgcgg ccgcccgagg atcgccatct cggacgagcg tgcgcagctc
    19921 ttggacaccg gcggcggcgt cgccaaggcg ctcccgctcc tcggcgcaga accgttcgtc
    19981 gtgcacaact cggacaccat ttggcacgag cagggcacgg acaacctccg ccgcctcatg
    20041 accttctgga gcccgcctcg catggacctc gccatgctgc ttgcacggcg cgatactagc
    20101 ctcggatacg atggccacgg cgatttcgag ctcgatgctg acgggcgcat ccgccgtcgc
    20161 gcaccgggca cgacggcgac ctacgtattc gccggcgtct cgatcatggc gccatcgctc
    20221 ctcgccgatg ctccgccagt cgccttctcg ctcaatcggc cctgggatca ggcaatcgct
    20281 tccggccgtg cctgcgcaat cgtgctcgac ggcacctgga tgcacgtcgg cgatccggca
    20341 gccctcgccg cggccgagcg cttcctcgga gcagcgcatg gccgcggctg acgccgtaca
    20401 ctcagaggcc gtcttcacgg cgccccccgg cgttccgttc ctcgatgccg tcgcccgcgc
    20461 catccttgcc ggagatctgc cgcgacctgg tggtgcgccg ccctcgatcg agggcctcgc
    20521 cgacgtcacc atcctgctgc cgacgcggcg cgcggtccga catctgaccg aggccattct
    20581 cgtcgcatcg gggcgacgtg cggtgctgct ccccaatatc aggccgatcg gcgagggcga
    20641 cgaggacttg ggcctgctcg acggcctgct tgctccaggc aacgccacag gcttcggcaa
    20701 ggatgcggcg cacctcgatc tcccgcccgc gatcagcgcg atcgagcggc gcctcacgct
    20761 gacgcgcctg gtcctcatgt ggtcgaaggc cttgcgtgcg cgcgccgaga ccgatgcccg
    20821 accgctctcc ggtccatcga cgcccgcaca ggcggcacgc tttgccgacg acctcggccg
    20881 cctcatcgac atgctcgaga ccgaaggcgt atcgccctcc cgcctcgctg gcctcgtgcc
    20941 ggatgccttc gcggagcatt ggcaggagac ggttgaattt
//
LOCUS       DAORNH010000038        20471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2026_length_20471_cov_2.801332, whole genome shotgun sequence.
ACCESSION   DAORNH010000038 DAORNH010000000
VERSION     DAORNH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20471
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_20471_cov_2.801332"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>363)
                     /locus_tag="PK264_06085"
     CDS             complement(<1..>363)
                     /locus_tag="PK264_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRD75495.1"
                     /translation="VAPGTSPEDRLAAEATELRRQAEAGLSRRLEVNPLADGFARADY
                     EKRLAYEVEIITKMKFPGYFLIVADFISWSKTQGIPVGPGRGSGAGSVVAWALTITDL
                     DPLRFGLLFERFLNPERVS"
     assembly_gap    364..405
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<406..1261)
                     /locus_tag="PK264_06090"
     CDS             complement(<406..1261)
                     /locus_tag="PK264_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRD75496.1"
                     /translation="MAEPAAAPKPVRFIHLKVHSAYSLLEGALPIGKIAKLAEAHGMP
                     AIGLTDTNNMFGALEFSDKLAGSGIQPIAGVNLAVDFGDQAKPETAVAMPAPAKPRRD
                     GLLTAFAMNEQGYANLMKLASHAHLSIPDGSDPFVSIDMIERHSAGLIILTGGPDGPI
                     DGALRDGQRPLAEARLNRLAALAGDRLYVEVQRHGLPAESDVEPDLIALAYAHDLPLV
                     AVNEPYFAAPEDYEAHDALLAIADGRLVTDSDRRKLSANHGFRSADEMATVFSDLPEA
                     LAATVEIAK"
     gene            1567..2193
                     /locus_tag="PK264_06095"
     CDS             1567..2193
                     /locus_tag="PK264_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HRD75497.1"
                     /translation="MAKLQTLFPTEIYHAALTGARERRLIADLEAAALSLAREDGAGR
                     AWCRRHGYRGYTSYASLGNLVDRASVYGELVAILDCHARDFARNLEFALDGQRLVLNS
                     LWVNILEPGGYHTAHIHPGSVISGTVYIRVPEGAGAIRFEDPRLAMMMAAPMRRERAS
                     EKRRTFVEVAPKPGTILLWESWLRHEVPENRARSRRVSLSFNYGLEAR"
     gene            complement(2168..3463)
                     /locus_tag="PK264_06100"
     CDS             complement(2168..3463)
                     /locus_tag="PK264_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75498.1"
                     /translation="MAASTVAPPLDGYARRLVILFGLVYFSQGIGQHVGLVHQPLQFY
                     FKEALGLNPAQVTEYLAVLTIPWMIKPLYGLVSDFIPLVGFKRKSWLLLTNMLATTGL
                     LWLTSQTSPAAIVTALLMTAIGTAASDVIIDAIMVENGKKMGLTARFQSVQWLWISIA
                     SIVSHLLGGQLASRFTPGTALHIAATITLIAPIAVMVSSWLIVREEKSEMALSEMKAT
                     AGSLLEAFKSKTLWLVIAFLGFWNFSPSFGTPWYFHQTDTLKFEQDFIGLLGAVASAG
                     SVLGAFAYWQVISKMPLRRQLVLAIVMGTIGTLLYLLLLTPHGFTKPLAIAIDFMLGA
                     AAIMALLATLTLAAQACPPKAEGFTFAALMSVLNGFAQLAAIVGARLYTNVLDKSMAP
                     LIIISALFTAACFLLLPLLRAVNIDAVPASESSAPPAHN"
     gene            complement(3479..4291)
                     /locus_tag="PK264_06105"
     CDS             complement(3479..4291)
                     /locus_tag="PK264_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75499.1"
                     /translation="MSVLTRLGTRMKTALAGAAALALTGCIDIKATFFIDQAGMLGIE
                     GDVKLQPELDDVMQAVEAMFALDPEGRKFAGEGLCKFLEKTAATDPGGQRLEIKTRGF
                     KAGNRPACSLSMKFGPAQEILENIARELANLKEKVGLELAYEGTRRYRLTLDFGALHD
                     ADLRRMREGIIELLNNVRIPGVPTAPIQPAAVDRAMRKYQPMIIAMLKMGARGGEGDR
                     IDLGVRVPRIIATNMRQDGNTARVAMSYAELIALEQNVEIKRQHVYSVVFEF"
     gene            complement(4303..5091)
                     /locus_tag="PK264_06110"
     CDS             complement(4303..5091)
                     /locus_tag="PK264_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75500.1"
                     /translation="MLSRRVSARLRFIGAILPATLGLSACIDLEAGLAVRQDGATSIE
                     ATVRLDPEMQDVFGFAEAVAQIRPEIAVFTRNGLCPALEFAAALNPEMKMNVRAKQGV
                     ENERLFCAFDIEADTVDNILRNISPDAPITQIGILKVTQPGARRVRVDVDLGAIPDFT
                     PDLAKELARGALRGLGSATDAPDPAALEKFARLGKTASVSMTKLVLRDRTLKLTISAP
                     KIVETNGVTSGQKVTFSWPWTEVVDMAMQPQKRTGRRYFVVIEY"
     gene            complement(5182..6546)
                     /gene="glmM"
                     /locus_tag="PK264_06115"
     CDS             complement(5182..6546)
                     /gene="glmM"
                     /locus_tag="PK264_06115"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRD75501.1"
                     /translation="MTQRLFGTDGIRGHANRHPMTSEVALKVGMAAGQIFSRKASGHR
                     HRVVIGKDTRLSGYMIESALLSGFTSVGMDVFLLGPMPTPAVAMLTRSLRADLGVMIS
                     ASHNHSDDNGIKLFDPDGFKLSDETERKIECLVAKPADELLVDASKIGRATRVESAQE
                     RYIEYAKRTLPKNIRLDGLRIVIDCANGAGYRVAPDALWELGAEVIKIGVEPNGRNIN
                     LNCGSTAPDLLCQKVREFRADIGIALDGDADRVVIVDETGHIVDGDQILAVIGESWHR
                     RQKLAAGGVVATVMSNLGLERYLKSVGLSLARTSVGDRYVVEHMRKHGYNVGGEQSGH
                     IVLSDFTTTGDGLVAALQVLAVKVASDKPVSEVCRRFEPLPQILRNVRYSEGRPLEKD
                     SVVKAIRDGERRLGTTGRLVIRPSGTEPVIRVMAEGEDESLVNQVVGEICAAVQAAAQ
                     AEAA"
     gene            6833..7870
                     /locus_tag="PK264_06120"
     CDS             6833..7870
                     /locus_tag="PK264_06120"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRD75502.1"
                     /translation="MTKLPLFPATVAGSLPKPAWLAEPEKLWAPWRLAGAELEQGKRD
                     AALIWLKLQEDAGLDILSDGEQFRMHFVHGFLERIEGIDWSKKTKMGIRNNRYTVDVP
                     TVTARLSRPRPVHAEEVRFVRAHTRRHIKVTLPGPMTIADTIADAHYGRRPDMAMAFA
                     DILNAEARELAALGVDVVQFDEPAFNVFMDEVSEWGIPALERAAEGLSCKSAVHICYG
                     YGIEANVKWKETLGSEWRQYEAIFPALNRSAIGQVSLEAQGSKVPLSLIRLLSDKEIL
                     VGAIDVATSRIETPEAVAAVLEEATKYADRERIQACTNCGLAPLPRQVAAGKLAALGA
                     GARLMREKFRA"
     gene            complement(7878..8867)
                     /gene="galE"
                     /locus_tag="PK264_06125"
     CDS             complement(7878..8867)
                     /gene="galE"
                     /locus_tag="PK264_06125"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRD75503.1"
                     /translation="MNILVTGGAGYIGSHMVLELIDAGETVVVLDNLTTGFRWAVAPE
                     AELVVGDIGDETLVRRVLREKKIDAVIHFAGSIVVPESVTDPLGYYLNNTVKSRALIA
                     AAVVEGVDKFIFSSTAAVYGNPRENPVGEDATPAPMSPYGTSKLMTELMLADAARAHK
                     FRFVALRYFNVAGADPKGRTGQSTPKATHLIKAACEAALGKRMHLEVFGTDYPTRDGT
                     CIRDYIHVKDLARAHSAALAHLAKGGASDIFNCGYGLGFTVLEVIEAVKKAHGKPFEV
                     RLAPRRAGDPAAIVAKSDKIRAALGWEPRHDKLDEIVAQALSWERGLSERRGG"
     gene            9133..10011
                     /locus_tag="PK264_06130"
     CDS             9133..10011
                     /locus_tag="PK264_06130"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRD75504.1"
                     /translation="MSKKPIRKAVFPVAGLGTRFLPATKAMPKEMLTVVDRPVIQHVV
                     DEARAAGIEHFIFVTGRNKGVIEDHFDSPFELERVLSERGKKNELDLLSRDLPGPGQT
                     SFTRQQAPLGLGHAVWCARDIIGEEPFALLLPDMLHHAQKPCLAEMIEAYAEHGGNQI
                     AVSPVPEDQTHLYGIVGVADTKAKVSAVTSMIEKPPRGTAPSNLHITGRYILQPGIFE
                     LLARQERGSGGEIQLTDAMIGLSRQAGQRFFAVRYEGRIYDCGSKIGFLTANIAYALA
                     RDDLGPSLKAELRRLL"
     gene            10192..11574
                     /locus_tag="PK264_06135"
     CDS             10192..11574
                     /locus_tag="PK264_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRD75505.1"
                     /translation="MSRDAADRKRQDGVTGHVTGKLTRRGLLGLTGAWLGSTSVLAQQ
                     PSWLDTIPGFSTPGSRPASDKRAPKSTTPEVLNDLRQDPTPIRGEETYEALERAIGHY
                     QDIASRGGWPIIPGTRMMRPGDDDERVMILRRRLQITGDLPGPAGNSAAYAFDGHLEE
                     AVRRFQERHGLRVSGRADGPTIAALNVPAAARVAQLRLNLQRVRELLAQRIEDRYVLV
                     NVPAFQLEAVEQHMVHLRHRVIVGRPDRQSPVVRATIKGVNFFPYWRVPDSVATLDLI
                     PRLMREPDYLAKEYIRVLSGNFNGPELDPSQIDWRTADPVRIKFRQDPGPQNALGLVR
                     IDMPNEHGVYMHDTPMKKLFESRGRAFSAGCVRVQDVFQLVAWIARDEPGFDKSKIDE
                     VLAAGIGYDIPLTRPIPVYFTYITAWAERSGRVEFRPDVYGRDGQHELVAGRDRDPSE
                     PAPPPQSLAP"
     gene            complement(11856..13187)
                     /gene="paaF"
                     /locus_tag="PK264_06140"
     CDS             complement(11856..13187)
                     /gene="paaF"
                     /locus_tag="PK264_06140"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HRD75506.1"
                     /translation="MENLTPERGTLDPIEIASREEIRALQLARLKWSVRHAYENVRHY
                     WEAFTAAGVTPEDIESLDDIARLPFTTKADLRANYPFGLFAAPREKMARIHASSGTTG
                     KPTVVGYTKRDVETWADLVARSIWAAGGRPGMTVHVAYGYGLFTGGLGAHYGAERLGC
                     TVIPVSGGMTERQVSLICDFKPDIIMVTPSYMLAILDEFRRQGIDPRATSLKVGIFGA
                     EPWTNAMRAEIEQAFDMHAVDIYGLSEVMGPGVANECVETKDGLHIWEDHFYPEIIDP
                     ETGAVLPDGERGELVFTTLTREGMPVIRYRTRDLTRLLPGSARSMRRMEKVTGRSDDM
                     MIVRGVNVFPTQIEELLLRVPALTPHYQVVLTRDDRLDEMEILVEATAEASVELRAKA
                     AAELAHHVKGLIGVSARVTVKDPGGVERSQGKARRVVDKRAKQPLTPSLST"
     gene            complement(13259..13972)
                     /locus_tag="PK264_06145"
     CDS             complement(13259..13972)
                     /locus_tag="PK264_06145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD75507.1"
                     /translation="MFTKSEAYYDAIYSWKNYEAEAGRIREIVAARQRSAGRALLDVA
                     CGTGGHVPYLREHFDITGVDLDPKMIEIARARHAGISFHVADMADVDLGRRFDVVACL
                     FSSIGYLKTPERLTQAIVTLARHLEPGGVLLIEPYFSPESWDMTRRPAKPMLVDRSDL
                     QIVRMIDWWREGDVIISDFHYLIGTAKGVEHFTERHEMALSRESDFRSALAAAGLEAE
                     FDAKGLMGRGLYIGLKPAT"
     gene            14175..15260
                     /locus_tag="PK264_06150"
     CDS             14175..15260
                     /locus_tag="PK264_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD75508.1"
                     /translation="MPRSINALCLSALLLALSACGESPTSPGEVRPVRTLIVDPKPLD
                     DDRQAVGEIRPRQESDLGFRISGKLVSRVVDVGAMIKKGDLLARLDEGDYQNRLRSAE
                     ADVASAEAVQSEAHGAEARYRQLLSTGAATRANYDAALKNLRSAEAKLDAAKAALALA
                     RDQVKYTELTADFDGIVTAVGAEPGQVVNIGQMIVRLALPGEKDAVFAIAESAFRDRP
                     PGDRPEIIVTLLSDPRVTTEGIVREISPVADPATRTFQVKVALKGAPEQMRFGSSVLG
                     RLKAMTAPVVVLPSSALFDKGGRPAVWVYLAASSTVSLKPVVVARFETDRVVVSEGLA
                     KGEVVVTAGVNRLREGQKVRAGAGSGP"
     gene            15257..>18181
                     /locus_tag="PK264_06155"
     CDS             15257..>18181
                     /locus_tag="PK264_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD75509.1"
                     /translation="MTSFNLSDWALRHKSLVAYLMLVSALVGIYSYGKLGREEDPPFT
                     IKTMLIKAVWPGATATETVTQITERIEKKLEEVAGLDFVRSYTKPGEAVIFVNLKDSV
                     RASEVPELWYQVRKKVGDIKSQLPSGVQGPFFNDEFGDTYSLIFAFTADGFTHRDLRD
                     HVDRVRSEVLRVADVAKVEFIGTQDEKIYLEFSTVQLAALGLDVNAIMQALQAQNAIT
                     PSGTIDAGPERIAIRVSGEFTSEASLKSINFRHGSRFFRLSDIAKVQRAYIDPPSPLF
                     RYKGEPAIGLAIAMTKNGDAEALGRNIKAKIGALTSDLPIGIDVHLVADQPHVVREAV
                     GEFTKTLWEAIAIVLAVSFFALGWRPGIVVAIAIPLVLAITFAFMYVLGISLQRISLG
                     ALIIALGLLVDDAMIAVEMMITKLEEGYDKVKAATFTYTSTAFPMLTGTLVTVCGFVP
                     VGFAQSGAGEYCFTMFIVISIALIASWVVAVLFTPLTGVYILPATMKGHGGHQKSGLS
                     VWFRDTLDWALRRKWSVLAATIGIFGLSLVGMRFVQSQFFPASDRPELLLNLTLPQSA
                     SIKATTEVADRVEKVLKSDPDIAYWSFYIGQGAVRFYLPLDAQLANDFFAQAVIVTKG
                     FKYRAKVIERLERALAGSDFDNVMARVAPLELGPPVGWPLKFRVSGPDAIMTREIAQR
                     FAIVLGGNARTRNINYDWNEPARVIKIEVDQDRARLLGITSEQLARQINSVLSGTTVT
                     QIRDHTYLIDIVARAIPEERAKIETLRNLRLSTAGGRTVPLVQIATLAYTLEPPLIWR
                     RQRLPTVTVQADVAPGVEAATVVKDLQTAITEFRSKLPPGYTVVIGGATEDSAKAEAS
                     IFAVFPLMLLLMVSILMVQLMSFQRLGLVMLTAPLAIIGVAAALLVSRAPMGFVAILG
                     IISLIGMVIRNSVILLDQIDQNISAGEEPWSAVLNATEHRLRPILLTAAAAILGRFRS
                     GEL"
     assembly_gap    18182..18191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18192..18407
                     /locus_tag="PK264_06160"
     CDS             <18192..18407
                     /locus_tag="PK264_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75510.1"
                     /translation="ILGMIPIAPTVFWGPMAYAVMGGLAVATALTLVFLPALYVAWFR
                     IIPPPSEPAAAEGRADRSPAGSVAAAA"
     gene            18484..19563
                     /locus_tag="PK264_06165"
     CDS             18484..19563
                     /locus_tag="PK264_06165"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HRD75511.1"
                     /translation="MASVTLDTVRKVYAGGVEAVKGVSLAIPDGSFTVLLGPSGCGKS
                     TLLRMIAGLERISGGTIRIGERIVNEVEPADRDIAMVFQNYALYPHMSVYDNMAYGLR
                     NRGVGKPEIETRVAAAAKMLAIEPFLKRKPRELSGGQRQRVAMGRAIVREPQAFLFDE
                     PLSNLDAKLRVQMRLEIRRLHNRLKATSIFVTHDQVEAMTLADTVVVMNQGRIEQVGA
                     PSDVYRAPASKFVATFIGSPAMSLIPGKVVSEGIVEVAGGALLKFATEAFDAPVGRTL
                     EAGIRPEDLTLTTASFGGLPLAIEFIEELGANRLVHGIVGDHPLVVSLPASDTAPPSG
                     TMQAIPRAGAVHLFDEKSGASLRKA"
     gene            complement(19627..19908)
                     /locus_tag="PK264_06170"
     CDS             complement(19627..19908)
                     /locus_tag="PK264_06170"
                     /inference="COORDINATES: protein motif:HMM:NF023518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HRD75512.1"
                     /translation="MLKKRRPPVDRERAEAIGLAALTFLAADEQRFAAFLAESGLDLA
                     TLKARAAEPDLLAAILDHLERDESALLVFGAERDVKVEEVALAKRLLEG"
     gene            20083..>20471
                     /locus_tag="PK264_06175"
     CDS             20083..>20471
                     /locus_tag="PK264_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75513.1"
                     /translation="MSSTRPPTTALLGPWNPGITSTLPRRLYPRVTVFSADNAVMTLR
                     DLEELAGFSGLPMQAISELRPERLVLHEVLVRAMTELSISDGPNQRELGVQFRRVVSA
                     ILARHIAPRMPALVAAHAALKVALAEV"
BASE COUNT         3395 a         6652 c         6811 g         3561 t
ORIGIN      
        1 cgagacgcgc tcgggattga ggaagcgctc gaagaggagg ccgaagcgga ggggatcgag
       61 atcggtgatg gtgagtgccc aggcgaccac cgagcccgcg cccgatccgc ggcccggccc
      121 gaccggaatg ccctgggtct tcgaccacga gatgaagtcg gcgacgatca ggaagtagcc
      181 ggggaacttc atcttggtga tgatttcgac ctcgtaggcg aggcgctttt cgtagtccgc
      241 ccgcgcgaag ccgtcggcga gcgggttcac ctcgaggcgg cgcgacagtc cagcttcggc
      301 ctggcggcgg agctcggtcg cctcggcagc gaggcgatcc tcaggcgagg tgccgggcgc
      361 gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcttg gcgatctcga
      421 ccgttgcggc aagcgcctcg gggaggtcgg agaacaccgt cgccatctcg tcggccgatc
      481 ggaagccgtg gttcgccgag agcttgcggc ggtcgctgtc ggtgacgagg cggccgtccg
      541 caatggcaag aagagcgtcg tgggcctcgt agtcctcggg tgctgcaaag tagggctcgt
      601 tgacggcgac gagcggcagg tcgtgagcgt aggcgagcgc gatcaggtcc ggctcgacgt
      661 cggactcggc cgggagcccg tggcgttgca cctcgacata gagcctgtcg ccggcgagcg
      721 ccgcgagacg attgaggcga gcttcggcga gggggcgctg cccgtcgcgc aaggcgccgt
      781 ctatcgggcc gtcggggcca ccggtcagga tgatgaggcc cgccgagtgc cgctcgatca
      841 tgtcgatgga aacgaatggg tcgctgccgt ccggaatcga gaggtgggcg tggctcgcga
      901 gcttcatgag gttcgcgtag ccctgctcgt tcatggcgaa tgccgtgagc agaccatcgc
      961 ggcggggttt cgcgggcgcc ggcatggcga cggccgtctc gggcttcgcc tggtcgccga
     1021 aatctacggc aaggttgacg cccgcgattg gttggatgcc tgatccggcg agcttgtccg
     1081 agaattcgag cgcgccgaac atgttgttgg tgtcggtaag gcctatcgcc ggcatgccat
     1141 gtgcctcggc gagcttggcg atcttgccga tcggcagcgc gccttcgagc agcgaatagg
     1201 ccgagtggac cttcagatgg atgaagcgca cgggcttggg tgcagcggcg ggctcggcca
     1261 tgatgctctc gggatcgcga atcgatgtcg acccttagtt taccctgcct ggccaggcgt
     1321 tgggcgcgct gaaaccttag ccgtggaatt gtcgacggct gggggttgtt ccatggcatg
     1381 gatgccgctc gcgtcgtgcc ggtggcgcgc atccagcgtg acgcactccg gtaaggggaa
     1441 tacccctgat gaccccgccc ttcgacacca aaacacgggc ggtctggcgg gtgtcccgcc
     1501 agcggggcgc ggggctggcc ccgcttcgcg aagcgaaagc gacggcgatt ggctgcaagg
     1561 acctaaatgg cgaagctgca gacgctcttt ccgaccgaga tctaccacgc tgccctgacc
     1621 ggcgcgcgcg agcgccgcct catcgcagat ctcgaggcgg cggctttgtc gctcgcgcgg
     1681 gaggatggcg cgggtcgcgc ctggtgccgc cggcatggct atcgcggcta cacctcttac
     1741 gcctcgctcg gcaacctcgt cgatcgcgcg tctgtctacg gcgagctggt cgcgatactc
     1801 gattgccatg cgagggactt cgcccgcaac ctcgagttcg ccctcgacgg ccagcgcctc
     1861 gtcttgaaca gcctgtgggt gaacatcctc gagcccggcg gctatcacac cgcccacatt
     1921 catccaggga gcgtgatctc cggcacggtc tacatccgcg tgcctgaagg ggcgggtgcc
     1981 atccgcttcg aggatccacg cctcgcgatg atgatggcag cccccatgcg gcgcgagcgt
     2041 gcttccgaga agcgccgcac attcgtcgag gttgcgccga agccgggcac gatcctgctc
     2101 tgggagagct ggctccgcca cgaggtgccg gagaaccggg cgaggagccg gcgcgtctcg
     2161 ctgagcttca attatgggct ggaggcgcgc tagactcgga agccggcacg gcgtcgatgt
     2221 tcacggcgcg gagcagtggc agcagcagga agcaggcggc ggtgaagagc gctgaaatga
     2281 tgatcagtgg cgccatcgac ttgtcgagga cgtttgtgta gagccgcgcg cctacgatgg
     2341 cagcgagttg cgcgaagccg ttcagtaccg acatcagggc cgcaaaggtg aagccctcgg
     2401 ccttcggcgg acaggcctga gccgcgagcg tcagcgtggc gaggagtgcc atgatggcgg
     2461 cggcaccgag catgaagtcg atcgcgatcg cgagcggctt cgtgaagccg tgcggcgtca
     2521 gcaggagcag atagaggaga gtgccgatcg tgcccatgac gatcgccagg acgagctggc
     2581 gacggagcgg cattttggag atcacctgcc agtaggcgaa ggcgccgagc acggagccgg
     2641 ccgaggcgac ggcgccgagc agtccgatga aatcctgctc gaatttcaac gtgtcggtct
     2701 ggtggaaata ccagggtgta ccgaagctcg ggctgaagtt ccagaagccg aggaaggcga
     2761 tgacgagcca gagcgtcttc gacttgaagg cctcgagcag gctgcccgcc gtcgccttca
     2821 tctcgctgag ggccatctcg cttttttctt cgcgcacgat gagccagctc gacaccatca
     2881 cggcgatcgg cgcgatcagc gtgatggtcg ccgcgatgtg cagcgccgtt ccgggcgtga
     2941 agcggctggc gagctgcccg ccgagcagat ggctcacgat cgaggcgatg ctgatccaga
     3001 gccactgcac gctctggaaa cgggccgtga ggcccatctt cttgccgttc tcgaccatga
     3061 tggcgtcgat gatgacatcg gatgcggctg tgccgatcgc cgtcatgagg agggctgtga
     3121 cgattgcggc cggcgaggtc tggctcgtga gccagaggag accggtggtc gccagcatgt
     3181 tggtgaggag cagccacgac ttccgcttga agcccacaag cgggatgaag tcgctgacga
     3241 gtccgtagag cggcttgatc atccagggga tggtgagcac cgcgagatac tcggtcactt
     3301 gcgccggatt gaggccgagc gcctccttga aatagaactg cagcggctga tggacgaggc
     3361 cgacatgctg gccgatgccc tgcgagaaat agacgaggcc gaacaggatg acgaggcggc
     3421 gcgcgtagcc gtcgagcggc ggggcgacag tcgaggctgc catcgcttgg ggctccgctc
     3481 agaactcgaa cacgaccgag tagacgtgct gacgtttgat ctcgacgttc tgctcaagcg
     3541 cgatgagttc ggcatagctc atggcgacac gggcggtatt gccgtcctgg cgcatattgg
     3601 tcgctatgat ccgcggcacg cgcacgccga ggtcgatgcg atctccctcg ccacccctgg
     3661 cacccatctt gagcatagcg atgatcatcg gctggtattt gcgcatggcg cggtcgacgg
     3721 ccgcaggctg aatgggggca gtgggcacgc ctgggatgcg aacgttgttg agcagctcga
     3781 tgatgccctc gcgcatccgc ctcaagtcgg catcgtggag cgcaccgaag tcgagagtca
     3841 ggcgatagcg gcgcgtgccc tcgtaggcaa gttcgagacc gaccttttct ttgaggttcg
     3901 caagttcgcg cgcgatgttc tcgaggatct cttgggccgg gccgaacttc atgctgagcg
     3961 agcaggccgg ccggttgccg gccttgaagc cgcgcgtctt gatctcgagg cgctgaccgc
     4021 cgggatcggt agcggccgtc ttctcgagaa acttgcagag gccctcgccg gcgaacttgc
     4081 ggccctcggg atcgagcgcg aacatggcct cgaccgcctg catgacgtcg tcgagctcgg
     4141 gctggagctt cacgtcgcct tcgatgccga gcatgccggc ctgatcgatg aagaaggtcg
     4201 ccttgatgtc gatgcagccc gtgagagcga gcgccgccgc gccggcgagc gcggtcttca
     4261 tccgggtgcc tagtcgtgtg agcacactca tcggtcagtc cctcaatatt cgatgacgac
     4321 gaaatagcgc cttccggtcc gcttctgtgg ctgcatggcc atgtcgacaa cctcggtcca
     4381 gggccagctg aaagttacct tctgaccgga ggtgacgccg ttcgtctcga cgatcttcgg
     4441 cgcactgatc gtgagcttca gggtgcggtc ccgaagcacg agcttcgtca tggagacgga
     4501 cgcggtcttg ccgaggcggg cgaatttctc gagcgccgcg ggatcgggcg cgtcggttgc
     4561 cgagccaagg ccgcgcagtg cgccgcgggc caactccttg gcgagatcgg gcgtgaaatc
     4621 ggggatcgcc ccgagatcga cgtcgacgcg gacgcgccgg gcgccgggct gcgtcacctt
     4681 gaggatgccg atctgcgtga tcggcgcatc gggcgagata ttccgcagga tgttgtcaac
     4741 cgtatcggcc tcgatgtcga aggcgcagaa caggcgctcg ttctccacgc cctgcttggc
     4801 gcggacgttc atcttcatct ctgggttgag ggcggccgcg aactcgagcg ccgggcagag
     4861 gccgttccgc gtgaagacgg cgatctcggg tcggatctgc gcgacggcct ctgcaaaccc
     4921 gaacacgtcc tgcatctcgg gatcgagacg caccgtggcc tcgatgctgg tggcgccgtc
     4981 ctggcggacg gcgaggcctg cctcgagatc aatgcaggcc gaaagaccga gggtcgcagg
     5041 caggatcgct ccgatgaacc gcagtcgcgc ggagacccgt cgtgacagca tcaatgcctc
     5101 cccagcaatg ctggagaacg gtttcatgct ttcagacgat gatcaagtct tgtcgcaatg
     5161 cgactgttac aggcgccgcg atcaggcggc ttccgcctgg gctgcggcct ggacggcggc
     5221 gcagatctcg ccgacgacct ggttgacgag gctctcgtcc tcaccttccg ccatgacgcg
     5281 gatgacgggc tccgtgcccg acgggcggat gacgaggcgt ccggtggtgc cgagccggcg
     5341 ttcaccatct cggatcgcct tcacgacgct gtccttctcg agggggcgac cttcgctgta
     5401 gcgaacgttc ctcaggatct gcggcagcgg ctcgaagcgc cggcagacct cgctcacggg
     5461 cttgtcggaa gcgaccttga cggccagcac ctggagcgcg gcgacgagcc cgtcacccgt
     5521 cgtcgtgaag tcagagagca cgatgtggcc cgattgctcg ccgccgacgt tgtagccgtg
     5581 cttccgcatg tgctcgacga cgtagcggtc gccgacggag gtgcgggcga gcgagaggcc
     5641 gacgctcttc aggtagcgtt cgaggccgag gttcgacatc acggtcgcga cgacgccgcc
     5701 ggccgcgagc ttctggcggc ggtgccagct ctcgccgatg acggcgagga tctggtcgcc
     5761 gtcgacgata tggccggtct cgtcgacgat gacgacgcgg tcggcatcgc catcgagcgc
     5821 gatgccgatg tcggcacgga actcgcgcac cttctgacag aggagatcgg gcgccgtgga
     5881 gccgcagttc aggttgatat tgcgcccgtt gggctcgacg ccgatcttga tcacctcggc
     5941 accgagttcc cacagcgcat cgggggcgac ccggtagccg gcgccgttgg cgcagtcgat
     6001 gacgatgcgc aacccgtcga ggcggatgtt cttcggcagc gtacgcttgg cgtactcaat
     6061 gtagcgctcc tgcgcgctct cgacgcgtgt ggcgcggccg atcttgctcg catcgacgag
     6121 caactcgtcg gcgggtttgg cgacgaggca ctcaatcttg cgctcggttt cgtcggagag
     6181 cttgaagccg tcgggatcga agagcttgat gccgttgtcg tcggagtggt tgtgggacgc
     6241 tgagatcatg acgccgaggt cggcgcgcag cgaccgcgtc aacatggcga cggctggggt
     6301 cggcatcgga ccgagcagaa agacgtccat tccgacggag gtgaatcccg agaggagggc
     6361 ggactcgatc atgtagccgg agagccgcgt gtccttgccg atgacgacgc ggtggcgatg
     6421 gccactggcc ttgcgcgaga agatctggcc ggcggccatg ccgaccttca acgcaacctc
     6481 ggaggtcatg ggatggcggt tggcgtgccc gcggatgccg tcggtgccaa acagtcgttg
     6541 cgtcatgggt gccctcgctc attccgccgc taagcggtca cgtacgcttc gccctgtcgc
     6601 tccggcgtgt agaacactgc cacagcttgc acagttgcag ttagcacgac aggccgattc
     6661 ggtcatgcaa tgtgcttgcc agcctcgcgc tgatgcacgc ggagcacatg cgagtggtgg
     6721 cggcggcttc ggcggttagg tgtttcgctc atgtgcctgc catgcaacca tgcagccgca
     6781 gcctgctgtt tggcttgaaa ggtttgctgc atcgtctgga gggccttcgc cgatgaccaa
     6841 gctcccgctc ttcccggcaa ccgtcgccgg cagccttccg aagcccgcct ggcttgccga
     6901 gcccgaaaag ctctgggcgc cgtggcggct cgccggcgcc gagctcgagc aggggaaacg
     6961 agacgccgcg ctcatctggc tcaagctcca ggaggacgcc ggcctcgaca tcctctcgga
     7021 cggcgaacag ttccgcatgc acttcgtgca cggcttcctg gagcggatcg agggcatcga
     7081 ctggtcgaaa aagaccaaga tgggcatccg caacaatcgc tacactgtgg acgtgccgac
     7141 ggtcaccgcc cggctctcgc ggccgcggcc cgtgcatgcc gaggaggtgc gcttcgtgag
     7201 ggcgcatacg aggcggcaca tcaaggtgac gctgcccggt ccgatgacca tcgcggatac
     7261 gatcgcggac gcgcactacg gccgccgccc cgacatggcg atggccttcg ccgacatact
     7321 gaacgcagag gctcgcgagc ttgccgccct cggcgtcgac gtcgtccagt tcgacgagcc
     7381 ggccttcaac gtgttcatgg acgaggtcag cgagtggggc atcccggcgc tcgagcgcgc
     7441 cgcggaggga ctctcctgca agtcggccgt gcacatctgc tacggctacg gcatcgaggc
     7501 gaacgtgaag tggaaggaaa cgctcggcag cgaatggcgc cagtatgagg cgatctttcc
     7561 ggcgctcaat cgctcggcga tcggtcaggt gtcgctcgaa gcgcagggct cgaaggtgcc
     7621 gctgtccctc atccgcctgc tctccgacaa ggagatcctg gtcggcgcga tcgatgtggc
     7681 aacgagcagg atcgagacgc cggaagctgt ggcggcggtt ctggaggagg cgaccaagta
     7741 tgcggaccgc gagcgtatcc aggcctgcac gaactgcggg cttgcgcccc tgcctcggca
     7801 ggtggcagca gggaagctcg ccgcactcgg cgcaggggcc cgactgatgc gggaaaagtt
     7861 ccgagcctga cgcggcctca accgccgcgg cgctcactga gcccgcgctc ccagctcaag
     7921 gcctgcgcga cgatctcgtc gagcttgtcg tgccgcggct cccagccgag ggccgcgcgg
     7981 atcttgtctg acttggcgac gatggcggcg gggtcgcctg cccgacgtgg cgccaggcgc
     8041 acctcgaatg gcttgccgtg ggccttcttc accgcctcga tcacctcgag caccgtgaag
     8101 cccaggccat agccgcagtt gaagatgtcg gaggcgccgc ccttggcaag gtgtgcaagc
     8161 gccgccgagt gggctcgcgc gaggtccttc acgtggatgt agtcgcggat gcaggtgccg
     8221 tcgcgggtgg ggtagtcggt accgaacacc tcgagatgca tgcgcttgcc gagggctgcc
     8281 tcgcaggcgg ccttgatgag gtgggtggct ttcggcgtcg actggccggt gcggcccttc
     8341 gggtcggcgc ccgcgacgtt gaagtagcgc agcgccacaa agcggaactt gtgcgcgcgc
     8401 gccgcgtcgg cgagcatgag ctcggtcatg agcttcgacg tgccgtaggg cgacatcggc
     8461 gccggcgtcg catcctcgcc gaccgggttc tcgcgcgggt tgccgtagac ggcggccgtg
     8521 gacgagaaga tgaacttgtc gacgccctcg acgaccgctg cggcgatgag ggcgcgcgat
     8581 ttcacagtgt tgttcagata gtagccgagc gggtcagtga cggactcggg aacgacgatc
     8641 gagccggcga agtgaatgac ggcgtcgatc ttcttctctc tgagaaccct gcggacgagc
     8701 gtctcgtcgc cgatatcgcc gacaacgagc tcggcctcgg gcgctaccgc ccagcggaag
     8761 ccggtcgtca ggttgtcgag cacgacgacc gtctcgccgg catcgatgag ctcgaggacc
     8821 atgtggctgc cgatgtagcc ggcgccgccg gtgacgagga tattcatgtc tgcccctgca
     8881 acctgatgct ggccgagctg ccgtgtcgca cggcgcgcgg cccgatgcca tcccgtgttt
     8941 gtcgcgatgg cgcgacaaga ctttcttaat cgcaagcaac gcgaggggca cgatctggcc
     9001 ttgcgcgtgc atcgatagga tgcaaggcga ataccagcag acgcgtctcg gtgtcatgac
     9061 gacgtcgcag tggaatcagc aagcactcgc gtgcgtgaat tcggacgatc agcctttcgc
     9121 ggagctgcca gaatgtcgaa gaagcccatt cgcaaggccg tattcccagt cgcgggcctc
     9181 ggcacgcgct tcctgcccgc gaccaaggcg atgccgaagg agatgctgac cgtggtcgat
     9241 cgcccggtca tccagcatgt cgtcgacgag gcgcgcgcgg ccggcatcga gcacttcatt
     9301 ttcgtcaccg ggcggaacaa gggcgtcatc gaggaccatt tcgacagccc cttcgagctc
     9361 gagcgggtgc tgagcgagcg gggcaagaaa aacgaactcg acctcctctc gcgcgatctg
     9421 ccggggcccg gccagacgag cttcacgcgc cagcaggcgc cgctcgggct cgggcatgcg
     9481 gtctggtgcg cgcgcgatat catcggcgaa gagccgttcg cgctgctgct tcccgacatg
     9541 ctccatcatg cgcagaagcc ctgcctcgcc gagatgatcg aggcctacgc agagcatggc
     9601 ggcaatcaga tcgccgtctc gcccgttccg gaggatcaga cgcacctcta cggcatcgtc
     9661 ggcgtggcgg acacgaaggc aaaggtctcc gcggtgacga gcatgatcga gaagccgcca
     9721 cgggggacgg ctccctcaaa tctgcatatc accggccgtt acatcctcca gcccgggatc
     9781 ttcgagctgc ttgcgcgcca ggagcgcggt tcgggcggcg agatccagct caccgacgcc
     9841 atgatcgggc tttcgcgtca ggccggacag cgcttcttcg cggtgcgcta cgagggccgg
     9901 atctacgact gcggctcgaa gatcggcttc ctcacggcga atatcgccta tgcgctggcg
     9961 cgggacgacc ttggcccgag cctcaaagcg gagctgcgcc ggctgctttg aagtttcaac
    10021 gggttcggca atgtcactct gtcgtgagca atgttgaaca gcttccgcgc catcgggctt
    10081 gcacggtcat caaatcgacc ataagtctcg ctccaacgat cgagggtgct cggcatccga
    10141 ccgtgagggt gctggatgtg ctccagcgac agatgagcag gcgcggacgt catgagcagg
    10201 gacgcagccg atcgcaagcg gcaggatggc gtgacaggcc acgtgacagg taagctgacg
    10261 cggcggggcc tcctcgggct cacgggcgcc tggctcggct ctacgagcgt gctggcacag
    10321 cagccgagct ggctcgacac cattcccggg ttctccacac cgggctcgcg cccggcttcg
    10381 gacaagcgcg cgccgaagag cacgacgccg gaagtactca atgacctgag gcaggatcca
    10441 acgccgatcc ggggcgagga gacctacgag gcgctcgagc gcgccatcgg ccactatcag
    10501 gacatcgcca gtcgtggcgg atggccgata attccgggga cgcgtatgat gcgtccgggc
    10561 gacgacgacg agcgggtgat gatcctgcga cgtcgcttgc agatcactgg cgatcttccc
    10621 gggcctgccg gcaacagcgc ggcctatgcc ttcgacggtc acctcgagga ggcggtgcgc
    10681 cgtttccagg agcggcatgg cctccgcgtc agcggccgcg ccgacggccc gacgattgcc
    10741 gcactcaatg ttccggcagc cgcgcgcgtc gcccagctgc gtctcaattt gcagcgtgtg
    10801 cgcgagctgc tcgcgcagcg catcgaggat cgctacgttc tcgtcaacgt gccggcgttc
    10861 cagctcgagg cggtcgagca gcacatggtg catctccgcc accgcgtcat cgttggtcgg
    10921 ccggaccggc agtcgccggt ggtgcgtgcg accatcaagg gtgtgaattt ctttccgtac
    10981 tggcgcgttc ccgacagcgt tgcgacgctc gacctgattc cgcgtctgat gcgcgagccc
    11041 gactatcttg ccaaggaata catccgggtt ctcagcggca atttcaacgg cccggagctc
    11101 gatcccagtc aaatcgactg gcggacggcg gatcccgtgc ggatcaagtt ccgccaggac
    11161 ccgggaccgc agaacgcgct cggcctcgtg cgcatcgaca tgccgaacga gcatggcgtc
    11221 tacatgcacg atacgccgat gaagaagctc ttcgagtcgc gcggtcgcgc cttcagcgcc
    11281 ggctgcgtgc gcgtgcaaga cgtgtttcag ctggtcgcct ggatcgcgcg tgacgagccg
    11341 ggcttcgaca agtcgaagat cgacgaggtt ctggccgccg gcatcggcta tgacatcccg
    11401 ctgacgcgcc ctattccggt ctatttcacc tacatcaccg cctgggcaga gcgctcgggc
    11461 cgggtcgaat tccggcccga cgtctacggc cgcgacggcc agcatgagct ggtcgcgggt
    11521 cgcgaccgcg atccgagcga gcccgcgccg ccgccgcagt ccctggcacc ttgagtctgg
    11581 gtccagggac tctggcgcat aggcgccaac atagccgggg cagatttcga tagttttcga
    11641 agcgtcgcca cctaaacggg tcagcgtcgt cgtccaaagc tcgttgagca gcgatccatg
    11701 tgtcgcgtcg ccatgctgga atccaatttc attccaacag gcaagaacta tcttggcgcg
    11761 tattggactt gtctctggac cctattccga attggcagag cgccaaggag ggtcggcatt
    11821 cgcaaaggcc gccgcctact cctactcccc gctcttcagg tggagaggga cggcgtgagg
    11881 ggctgctttg cccgcttatc caccacgcgg cgcgctttcc cctggctgcg ctcgacgccg
    11941 cctgggtctt tcaccgtgac gcgcgcgctg acgccgatga ggcctttgac gtggtgggcg
    12001 agctcggccg cagccttggc gcgaagctcg acgctcgcct ccgctgtcgc ctcgaccaga
    12061 atctccatct catcgagccg gtcgtcgcgc gtcagcacga cctgatagtg cggcgtcagc
    12121 gccggtacgc gcagcaagag ctcctcgatc tgcgtcggga acacgttgac gccgcgcacg
    12181 atcatcatgt cgtcggagcg gccggtcacc ttctccatgc gccgcatcga tcgcgcagag
    12241 ccggggagga gccgcgtcag gtcgcgcgtc ctgtagcgga tcaccggcat gccctcgcgc
    12301 gtcagcgtcg tgaacacgag ttcgccccgc tcgccgtcgg gcagcacggc gcctgtctcg
    12361 ggatcgatga tctcgggata gaagtggtcc tcccagatgt gcaggccgtc cttcgtctcg
    12421 acgcactcgt tggcgacgcc gggccccatc acctccgaga gcccatagat gtcgacggcg
    12481 tgcatgtcga aggcctgctc gatctcagcg cgcatggcgt tggtccaggg ctcggcgccg
    12541 aaaatgccga ccttcagcga cgtcgcgcgc ggatcgatgc cctgccggcg gaactcgtcg
    12601 aggatcgcca gcatgtagga gggcgtcacc atgatgatgt ctggtttgaa gtcgcagatg
    12661 aggctcactt gccgctcggt catgccgccc gagaccggga tcaccgtgca gccgagcctt
    12721 tccgcgccat agtgcgcgcc gagcccgccc gtgaagaggc cgtagccgta ggcgacatgg
    12781 accgtcattc cgggcctgcc gccggccgcc cagatcgagc gggcaacgag gtccgcccac
    12841 gtctcgacgt cacgcttcgt gtagccgacg acggtcggct tccccgtcgt gccggacgag
    12901 gcatggatgc gcgccatctt ttcgcggggc gcggcgaaga ggccgaaagg atagttggcg
    12961 cgcaggtccg ccttggtcgt gaacgggagc ctggcgatat cgtcgagcga ctcgatgtcc
    13021 tccggcgtca caccggccgc ggtgaaggcc tcccagtagt gcctgacgtt ctcgtaggcg
    13081 tggcggaccg accacttgag tcgcgccagt tggagagcgc ggatctcctc gcgcgaggcg
    13141 atctcgatcg ggtcgagggt gccacgctcc ggcgtcaggt tctccacgtc gtcctcccgc
    13201 gttgcatttt ttgcgactat aggagggcgt cccccggcat tggaagcggc tcggcggctc
    13261 atgtcgctgg tttcagcccg atatagagtc cgcgccccat cagacctttc gcgtcgaact
    13321 cggcctcgag acctgccgct gcaagcgcgg accggaagtc gctctcacgc gagagcgcca
    13381 tctcgtggcg ctcggtgaaa tgttcgacac ccttggctgt cccaatgaga tagtggaaat
    13441 ccgagatgat cacgtcgccc tcgcgccacc agtcgatcat gcgcacgatc tgaagatccg
    13501 accgatcgac gagcatcggc ttggccggcc gtcgtgtcat gtcccagctc tccggcgaga
    13561 agtagggctc gatcagcagg acgccgccgg gctccaggtg acgcgccaag gtgacgatgg
    13621 cttgcgtgag acgctcgggc gtcttcaggt agccgatcga cgagaagagg cacgccacga
    13681 cgtcgaaacg ccgtccgagg tcgacgtccg ccatgtcggc gacatggaag gagatgcccg
    13741 catgccgcgc cctggcgatc tcgatcatct tcggatcgag atcgaccccc gtgatgtcga
    13801 agtgctctct gagatacggc acgtgcccgc ctgtcccgca ggcgacatcg aggagcgcgc
    13861 gcccggccga gcgctggcgc gccgccacga tctcgcggat gcgccccgcc tcggcctcgt
    13921 aatttttcca cgaatagatc gcgtcgtagt aggcttcgga tttcgtgaac atcagtagtc
    13981 acctcaagca acgagcgagc tccggccaac tggcgcacca cgtgggcgag cgtcctggag
    14041 ccggtcacgg cagctcgaac acctggcaat ttcctgtgca attttctgtg gaacccggca
    14101 aggtccgaag gtagcatcga gcactccact ggtccatttc gccaagctca cggccttatc
    14161 gcgggacgag cgccatgcct cggtcgatca atgccttatg cctgtcagct ttgcttctcg
    14221 cactctcggc ctgcggcgag agcccgacaa gtcccggtga ggtccggccc gtacgcacgc
    14281 tcatcgtcga tccgaagccc ctcgacgacg atcgccaggc cgtcggcgag atcaggccgc
    14341 ggcaggagag cgacctcggc ttccgcatct cgggcaagct cgtctcgcgg gtcgtggacg
    14401 tcggcgcgat gatcaagaag ggcgatctgc tggcgcggct cgacgagggc gactatcaga
    14461 accgcctccg gtcagcagag gccgacgtcg cttcggcgga agccgtgcaa tccgaggctc
    14521 acggggccga ggcccgctac cgccagctgc tctcgaccgg cgctgcgacg cgggccaact
    14581 acgatgctgc gctcaagaac ctcagatccg ccgaggcgaa gctcgatgcg gccaaggctg
    14641 cccttgccct cgcccgtgac caggtcaagt acacggagct cacggccgat ttcgatggca
    14701 ttgtcaccgc cgtcggcgcc gagccaggtc aggtcgtcaa tatcgggcag atgatcgtcc
    14761 gacttgccct gccgggcgag aaggacgcgg tattcgccat cgcagaatcg gcattccgtg
    14821 accggccgcc gggcgatcgt cccgagatca tcgtgacact gctgagcgac cccagagtga
    14881 cgaccgaagg tatcgtgcgc gaaatctcgc ccgtggccga tccggcgacg cgaacctttc
    14941 aggtgaaggt cgccctgaag ggcgcgccgg agcagatgcg tttcggctcg agcgtgctcg
    15001 gtcgcctcaa ggccatgacc gctcccgtcg tcgtgctgcc gagcagcgcg ctcttcgaca
    15061 agggcggccg accggcagtc tgggtttatc tcgccgcctc cagcaccgtt tcgctgaagc
    15121 ccgtcgtggt ggcccgcttc gagaccgatc gcgtcgtcgt cagcgagggg ctggcaaaag
    15181 gcgaggttgt ggtcacggcc ggcgtcaacc ggttgcgcga aggccagaaa gtccgcgcag
    15241 gtgccgggag cggcccatga cgtcgttcaa cctttcggac tgggccctga ggcacaagag
    15301 cctcgtcgca tacctgatgc tggtgtcggc gctcgtcgga atctactcct acggcaagct
    15361 tggccgcgag gaggacccgc ccttcaccat caagaccatg ctgatcaagg ccgtatggcc
    15421 cggcgccacg gcaacggaga cggtcacaca aatcaccgag cggatcgaga agaagctcga
    15481 ggaggtggcg ggcctcgatt tcgtccgcag ctacacgaag cccggagagg ccgtcatctt
    15541 cgtcaacctg aaggacagcg tccgcgcctc ggaggtgccg gagctctggt atcaggtcag
    15601 aaagaaggtc ggcgacatca agtcccagct gccctcgggc gtccagggtc cgttcttcaa
    15661 cgacgaattc ggcgacacct actcgctcat cttcgcgttc acggcggacg gcttcacgca
    15721 tcgtgacttg cgggaccatg tggaccgggt gcgctccgag gtgctgcgcg tcgcggacgt
    15781 cgcgaaggta gagttcatcg gcacgcagga cgagaagatc tacctcgagt tctcgaccgt
    15841 tcagctcgcc gcactcggtc tcgatgtcaa cgcgatcatg caggccctgc aggcccagaa
    15901 cgcgatcacg ccgagcggca cgatcgatgc ggggcccgag cgcatcgcca tccgcgtgtc
    15961 gggggagttc acctccgagg cgagcctcaa gtccatcaac ttccgccatg gcagccgctt
    16021 cttccggctg agcgacatcg ccaaggtgca gcgcgcctac atcgacccgc cgagcccgct
    16081 gttccgctac aagggcgagc cggccatcgg gctcgctatc gccatgacga agaacggcga
    16141 tgccgaagca ctcggccgaa atatcaaggc gaagatcggc gcgctcacga gcgatctgcc
    16201 catcggcatc gacgtgcatc tcgtcgccga tcagcctcac gtcgtgcgcg aggctgtcgg
    16261 cgagttcacg aagacgctct gggaggccat tgcgatcgtg ctggccgtga gcttctttgc
    16321 gctcggctgg cgtccgggaa ttgtcgtcgc gatcgccatc ccgctggtcc tcgcgatcac
    16381 atttgccttc atgtacgtgc tcggcatctc gctccagcgc atctcgctcg gcgcgctgat
    16441 catcgccctc ggcctcctcg tcgatgatgc catgatcgcc gtcgaaatga tgatcaccaa
    16501 gctcgaggag ggctacgaca aggtgaaggc ggccaccttc acctacacct cgaccgcctt
    16561 cccgatgctg accgggacgc tcgtcaccgt ctgcggcttc gtgcccgtcg gcttcgccca
    16621 gagcggcgcc ggcgagtatt gctttacgat gttcatcgtg atctcgatcg cgctcatagc
    16681 gtcgtgggtg gtcgccgtcc tgttcacgcc gcttactggc gtctatatcc tgcccgccac
    16741 catgaaagga cacggcgggc accagaaatc cggcttgtcg gtctggtttc gtgacacgct
    16801 cgattgggcg ctcaggcgca aatggtccgt cctcgctgcg accattggca tctttggcct
    16861 gtctctcgtc gggatgcgct tcgtgcagag ccagttcttc ccggcctcgg accggcccga
    16921 gctgctcctc aatctgacgc tgccgcagag tgcgtcgatc aaggcgacga ccgaggtggc
    16981 ggaccgtgtc gagaaggtcc tgaagtccga tcccgacatt gcctactgga gcttctacat
    17041 cggacagggc gccgtccgct tctacctgcc gctggacgct cagctcgcca acgatttctt
    17101 tgctcaggcg gtcatcgtga ccaaggggtt caagtatcgc gcgaaggtga tcgaacgtct
    17161 ggaaagggcg ctcgcgggca gcgacttcga caacgtcatg gcgcgcgtcg ctccgctcga
    17221 gcttgggccc ccggtcggct ggccgctgaa attccgcgtg agcgggccgg atgcaatcat
    17281 gacacgagag atcgcccagc gtttcgccat cgtcctcggt ggcaacgccc ggacgcgcaa
    17341 catcaactac gactggaacg agccggcgcg ggtgatcaag atcgaggtcg atcaggatcg
    17401 cgcccggctc ctcggcatca cgtcggagca gctcgcgcgg cagatcaact ccgtgctgtc
    17461 cggcacgacc gtcacccaga tccgcgatca tacctatctc atcgacatcg tggcgcgcgc
    17521 catccccgag gagcgggcaa agatcgagac cttgcgaaac cttcgcctgt cgacggctgg
    17581 cggccgcacg gtaccgctgg ttcagatcgc gacgctcgcc tacaccctcg agccgccgct
    17641 catctggcgt cgccagcgcc tcccgactgt caccgtccag gccgatgtcg cgccaggcgt
    17701 cgaggccgcg accgtcgtga aggatcttca aaccgcgatc acggagttcc gctccaagct
    17761 cccgcccggc tacactgtag tcatcggcgg cgccaccgag gatagcgcca aggcggaagc
    17821 gagcattttc gccgtgttcc cgctcatgct gctgctgatg gtgtcgatcc tgatggtgca
    17881 gctcatgagc ttccagcgcc tcggcctcgt gatgctgacg gcccctctgg cgatcatcgg
    17941 cgtcgccgca gccctgctcg tctcgcgcgc gccgatgggc ttcgtcgcca tcctcggcat
    18001 catctccctc atcggcatgg tgatccgcaa ctccgtcatc ctgctcgacc agattgacca
    18061 gaatatctcc gccggcgagg agccgtggtc ggccgtcctc aacgcgaccg agcatcgcct
    18121 ccggcccatt ctgctcacgg cggcggcggc gatcctcggc cgcttccgct cgggcgagct
    18181 cnnnnnnnnn natcctcggc atgatcccga tcgcgccgac cgtattttgg ggaccgatgg
    18241 cctacgcggt catgggcggc ctcgcggtcg caaccgcgct gacgctcgta tttctcccgg
    18301 cactctacgt cgcctggttc cgcatcatcc cgccgccgtc ggagccggca gccgccgaag
    18361 gacgcgccga ccgatcgccg gccggcagcg tcgccgctgc ggcttgagcc tctttactgt
    18421 ttcattcggt ggacagccgg ttgcccggtt cgctaccttg tgcgcaggac gcgaggggga
    18481 cgcatggcat ccgtgacgct tgacactgtg cgcaaggtct acgccggcgg cgtcgaggcc
    18541 gtgaagggcg tctcgctcgc gatcccggac ggctcgttca ccgtgctgct cgggccgtcg
    18601 ggctgcggaa aatccaccct gctccgcatg atcgccgggc tcgagcgcat ctcgggcggt
    18661 accatcagga tcggggagcg catcgtcaac gaggtcgagc ccgccgaccg cgacatcgcc
    18721 atggtcttcc agaactacgc gctttacccg cacatgtcgg tttacgacaa catggcctat
    18781 ggcctgcgca accgcggtgt cggaaagcca gagatcgaga cgcgagtcgc cgccgccgcc
    18841 aagatgctgg cgatcgagcc gttcctgaag cgcaagccgc gtgagctctc cggcggccag
    18901 cgccagcgcg tcgccatggg ccgcgccatc gtgcgcgagc cgcaggcgtt cctgttcgac
    18961 gagccgctct ccaacctcga cgcgaagctg cgcgtgcaga tgcgcctcga gatccgccgc
    19021 ctgcacaacc gcctgaaggc gacgtcgatc ttcgtcacgc acgaccaggt cgaggcgatg
    19081 acgctcgccg acaccgtcgt cgtcatgaac cagggccgca tcgagcaggt gggcgcgccc
    19141 tccgacgtct accgcgcgcc tgcctcgaag ttcgtcgcga ccttcatcgg ctcgccggcc
    19201 atgagcctca ttccgggaaa ggtcgtctcc gagggcatcg tcgaggttgc gggcggcgcg
    19261 ctcctcaagt tcgcaaccga agccttcgat gccccggtcg ggcgaacgct cgaagccggc
    19321 atccggcccg aggatctgac gctcaccacc gcgagcttcg gcggcctgcc gctcgcgatc
    19381 gagttcatcg aggagctcgg cgccaaccgc ctcgtccacg gcatcgtcgg cgatcacccc
    19441 ctcgtcgtct ccctgcccgc ctctgacacc gcgccgccga gcggcaccat gcaggcgatc
    19501 ccgcgcgccg gcgccgtgca cctcttcgat gagaagagcg gcgcgagctt gcggaaggcg
    19561 taaggtagac gaggtctccg cctctcgacc ccgccaggga cttggtccct ggacccaaac
    19621 ttggtttcac ccctccagca gccgcttcgc cagcgccact tcctccacct tcacgtcgcg
    19681 ctcggcgccg aacacgagga gcgcgctctc gtcgcgttcg aggtggtcga ggatcgcggc
    19741 gaggaggtcg ggctcggcgg cgcgggcctt gagtgtcgca aggtcgaggc cgctttcggc
    19801 gaggaaggcg gcgaaacgct gctcgtcggc ggcgaggaag gtgagcgccg cgaggccgat
    19861 cgcctcggcc cgctcgcggt cgaccggcgg tcggcgcttc ttcaacattg gctacccctt
    19921 tgcgaatgga gatgcggggc tctgccccgc gccccgccaa aggccgtcat cgcgaagcga
    19981 tgctccgcat cgaggacctt tgggaaccgc tttgtagctg cggcgtcgcc agtctatcat
    20041 gaccgactgc gtaggcgggt tggggccgac gacggaggag ccatgagcag cacgcgcccg
    20101 ccgacgaccg cgctgctcgg gccgtggaac cccggtatca cgtccacgct gccgcgccgg
    20161 ctttatccgc gtgtcaccgt gttcagcgcc gacaacgccg tgatgacgct ccgcgatctc
    20221 gaggagctcg cgggcttctc gggcctcccg atgcaggcga tctcggagct ccgccccgag
    20281 cgcctcgtgc tccacgaggt gctggtgcgc gccatgaccg aactctcgat cagcgacggc
    20341 ccgaaccagc gcgagctcgg cgtgcagttc cgccgcgtcg tctcggcgat cctcgcgcgc
    20401 cacatcgcgc cgcgcatgcc agcactggtc gccgcccacg cagcactcaa ggtcgcgctt
    20461 gcggaggtca t
//
LOCUS       DAORNH010000039        20311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2049_length_20311_cov_2.778633, whole genome shotgun sequence.
ACCESSION   DAORNH010000039 DAORNH010000000
VERSION     DAORNH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20311
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2049_length_20311_cov_2.778633"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..154
                     /locus_tag="PK264_06180"
                     /pseudo
     CDS             32..154
                     /locus_tag="PK264_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
     gene            175..471
                     /locus_tag="PK264_06185"
     CDS             175..471
                     /locus_tag="PK264_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRD75514.1"
                     /translation="MFRKCRSCHEVGPDAKTRLGPTLNGLFGRKAGSIEGFKYSPGNL
                     EAGSKGLVWTEETLFKYLEDPRAFVPGTTMAFAGLADEQDRRDVIAYLKQFSKK"
     gene            678..2168
                     /locus_tag="PK264_06190"
     CDS             678..2168
                     /locus_tag="PK264_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD75515.1"
                     /translation="MRSRLMGLLEKIGLLSLALLIAMGIGGTRLAQSQSSDADEIKVL
                     QYLLLWSGDYAGQIDGFVDPMLQQAVRDFQARAGVRDKAFDSGQAKQLVDEATQAAKR
                     VGYAYILDEATKAHVALPRDILSDGKANGKGSRFTTTDNLVEVETIRYEPGGDVTLLQ
                     LYRKFLEPLDRSALIQNSFRGDHFVLAWFVGSRQHLVRMHDRSGEVRGIEIRFDRNAR
                     SAFYPIAVAMVHDYSPFAPQSAVAALRQPKITSTLAPSDDPPPRGEPRRGRPGPGNTG
                     PIGPSPRRGSVSISSGSGFIVSPRGHILTNAHVVDDCTSISTGIHKSVKLVGIDKRND
                     LALLLPPGTPPPNIVVAKFASEAVRLGEDVATFGFPLRDILADQLNMTTGIVSSLAGL
                     RGDPKFIQISAPVQRGNSGGPVVDLAGRVIGVVTAKLDALGIAKASGGDLPQVVNFAV
                     RHELGISFMRRYGVEPILAPETEAMRKRDLAKLAADFTLAITCEKR"
     gene            3014..3646
                     /locus_tag="PK264_06195"
     CDS             3014..3646
                     /locus_tag="PK264_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRD75516.1"
                     /translation="MAVSPDPSSLATSGATVSLPNIITLCRIGLVPVIFWAFVSGRPV
                     LAFAAFVVAGISDAVDGFLAKTFDMKTELGAYLDPIADKLLIVSIYVALGVVKELPLL
                     LVVLVVSRDILIVLGVLLAWLLDRPVRIAPSEVSRWNTAAQLLLVATVLADEAFKLQL
                     EAPWIGGASLGYCSVRDILVWSTGMLTILSLGDYARIWLRHMSGEAGAAG"
     gene            3718..4863
                     /locus_tag="PK264_06200"
     CDS             3718..4863
                     /locus_tag="PK264_06200"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRD75517.1"
                     /translation="MRIDRQALFWTAALGVVLVLLSMLKGMLLPFVAGFVLAYFLNPA
                     VDALERAGLGRTLASSLMVVAGVLLIAAMILVLAPILAEHVRLLSETLPGDLQRARAT
                     IEKFLADRLGPGFASIAAGIDKAMAALEANSSGLLSTAAQAIWSRGQAIVSMVSLLLV
                     TPLVVFYLLVDWHRMADRVDGWLPREHAPAIRAIATDVNAAMAAFIRGQGLICVILSI
                     FYAIALTLAGIPYGGIIGVTTGLLSFIPFVGWMLGLIVAAGVALAKHWPALGPLVLVV
                     AIFAAGMLLDSFLLSPRLVGERLGLHPLTLLFALAAFSYLFGTVGLMIAVPLSAAFVV
                     VARHGLKAYLASDVFQGEALPAPLARPTTPAAGALPVRNASSRGEMS"
     gene            4860..5549
                     /locus_tag="PK264_06205"
     CDS             4860..5549
                     /locus_tag="PK264_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HRD75518.1"
                     /translation="MSAPHQLVLDLGHRPALGAEDFHVTASNETAIALVDRWPNWPGP
                     AVYIQGPAKSGKTHLVNVWRHMSHAPVQAAEALDDTAIPLLLEQKALAVEDIDRGIAS
                     ERSLFHLLNAARENNARLLLTGRRPTGEIEIALPDLRSRLRALPIAVIEPPDDALLAA
                     LLVKHFSDRQLLVDPPVIAYLTQRMERSAAAAAALVEAIDRRALATHRRVTRALASEV
                     ILAREVGMSPE"
     gene            5629..7842
                     /locus_tag="PK264_06210"
     CDS             5629..7842
                     /locus_tag="PK264_06210"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRD75519.1"
                     /translation="MSMIKDDAGKAGQERSPRSATAADRYYNRELSWLQFNRRVLEEA
                     LNLRHPVLERLRFLAISASNLDEFYMVRVAGLIGQIRAGVPTLSQDGLTASEQLAGVN
                     RFVADLVSEHQACWTSLAAEMAKAGIEIVEPGSLVGFERDWLESEFIDRMFPILTPIA
                     VDPAHPFPFIPNKGLTIGVELQRDADGRTMHALLPIPSQLARFVRLPAAQPPKGERAR
                     ARFIAIETFIGMFLGRLFPRFTAKNQGAFRVLRDSDLGMQEEAEDLVRSFESALKRRQ
                     RGHAIRVELDGRMPAPLQRFVVEETEVGEAGVFVKEGMLGLVDTTELIVADRPDLMFK
                     PFTIRFPERIRALGGDCLAAIRRKDIVVHHPYESFDVVVQFLRQAVADPNVMAIKWTL
                     YRTSRDSPIVQALKEAVEAGKSVTAVVELKARFDEAANIRWARDLENAGVHVVYGFVE
                     LKTHAKLGLIVRREGSELATYSHIGTGNYHPQTARVYTDLSFFTADKAIGRDVTRILN
                     FVTGYAEPTELEVMSASPKGIREKISAHIDGEIAHAKAGRPAAIWMKMNSLVDQRIID
                     QLYAASAAGVPVDLIVRGICCLRPGIPGLSDNIRVKSIIGRFLEHARIYCFGNGHGLP
                     SPKAAVYISSADMMPRNLDRRVEAMVPLSNKTVHAQVLEQIMVANLKDNQQSWQVLGD
                     GSSRRLTAGDGERPFNAHEYFMTNPSLSGRGDALKTHGPPRVKIESRRVASKKSG"
     gene            7846..9378
                     /gene="ppx"
                     /locus_tag="PK264_06215"
     CDS             7846..9378
                     /gene="ppx"
                     /locus_tag="PK264_06215"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HRD75520.1"
                     /translation="MSSGNDRGRFSELEPLSVVDIGSNSVRLVVYEGAVRTPVPLFNE
                     KVHCGLGRAVASTGRLGDDAVQRALDALTRFRSINRILKVKNIRAIATAAVRDASNGA
                     EFIRQGERNLGLKIDVLSGLEEAELAALGVHMGFANPDGVTGDLGGGSLELIDVLDPK
                     GRVPVSLPLGGLRLIDASGGSLEQAMKITDEQLGGVSWLKQGEGRTFYAVGGSWRALA
                     RLHMDEHDYPLRVMHGYQIQAREALEFCERVRKAKKISDLKGAATVSKARREVLPQGA
                     LVLERIVKRMKPKDVVFSVFGIREGLLYQLLGPTERARDPLLAFCEDYARLRSRSVEH
                     ARELIAWTDQIFRDPGPGETEDERRFRHAACLISDIGWRAHPDYRGEQSLNVIAHAGL
                     SGIDHPGRVFLALTVYYRHVGPSGEDGGHDDFSERMKDIAPKRLVRRARILGAAIRTA
                     HMLSIGMPGIIDETRIGYARDKLVLKVPHAYQSLNGERLQRRLQALATELEKRAEIEI
                     VA"
     gene            9857..10756
                     /locus_tag="PK264_06220"
     CDS             9857..10756
                     /locus_tag="PK264_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRD75521.1"
                     /translation="MADDPYKVLGVARDASEDDIRRAYRKLAKELHPDLNPNNRASAE
                     ERFKRISAAYDIVGDPDKRAKFDRGEIDGNGDARRGYQRAYAGRGGTGGARADDLGFS
                     SIFEDLFGGRTGPTRARGGFSVRGQDVRYSLEVDFLEAVSGARKRVTLPDGGTLDLSV
                     PEGVENGQVLRLKGKGAQGVNGGDAGDALVEIKVRPHAQFRRAGDDIALELPITIDEA
                     VLGAKIEVPTATGRVSLTLPKGTSSGRVFRLKGKGVRNLATGVLGDELVTIRIVMPES
                     IDDNLAFFMSEWRQKNAYDPGRK"
     gene            10921..12123
                     /locus_tag="PK264_06225"
     CDS             10921..12123
                     /locus_tag="PK264_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75522.1"
                     /translation="MTAGKDAGAAKLSRRTLIKAASAAAAASTVPTGWAIAQGKPLKI
                     GLMLPYSGAFAKLGENIHTAIELLIAEKGGKLGGRTIQIVKLDDESKPDLAAQNAERL
                     VKREAVDVLIGTVHSGVQLGIHKVVKESGTLTIVPNAGNGAVTRNECAKNVFRMSFSN
                     WQPAHGMGLHLGKKGIKKAAWITWDYAAGTESRDGFKDGLEKGGGQLMQTLTLKFPET
                     NFQPLLAQIAGLGVEAVGAFFAGGPAAQFVKEYKAANISVPLCGSGFLTEGVLGAAGP
                     AAEGIETALHYGDGLDNAKNAAFRKAFKEKAGRDADVYAVQGYDSAQLLAVGLEAVKG
                     NMDDEAGLYKAMRAAKIESPRGPVSISAAQNVVHNIYLRKVVGAENKIIGVAAENLAD
                     PGTGCTLK"
     gene            12214..13017
                     /locus_tag="PK264_06230"
     CDS             12214..13017
                     /locus_tag="PK264_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75523.1"
                     /translation="MHPVDIDGHSIEVRTIPGDASLATLVFLHEGLGSVALWRDFPDK
                     VSRQLRAPAVIYSRVGYGQSSGLCGPRSPDFMHREALDVLPKLLDRLGITRPLLIGHS
                     DGASIAIIHAASSGREVAGAVLMAPHVLVEPVCTEAIGRALATYQRGDLRQRLARYHR
                     DVDDAFLGWARIWLSPTFKDWSLRSECGHLAVPLLLIQGRQDEYGSLLQLDVIEDEVR
                     RGGVAAGRPVPEIQRVELDDCRHSPHRDQEARVIAEIAKWSAGLRQRTT"
     gene            complement(13069..13662)
                     /locus_tag="PK264_06235"
     CDS             complement(13069..13662)
                     /locus_tag="PK264_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75524.1"
                     /translation="MAAQQEKPKISDKTVMALMNYAWQILPTQFTSTTGKKIEIDKKK
                     REENVVPLDVAREVILVGYNSAHAQLCELWEEQNANYHTMMRLEVAKKKWTEQQLLYI
                     STLHRMTIHFAAGKVRIVEKGPDEQQVILEPIEKAACDEAKRQKIKSEILTYIAKHGG
                     IATPPAPGTTPPPATAATPPPAASGPVPVSQPQPPKK"
     gene            complement(13923..>14304)
                     /locus_tag="PK264_06240"
     CDS             complement(13923..>14304)
                     /locus_tag="PK264_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD75525.1"
                     /translation="VMIPVNSSFASLNLAQAVLILGYEWLKQTGAATLGRVTTYEDPV
                     EEGLRIKKGRLASREEMTGFFEHLETELDRMGFLFPPHKRQTMVRNIRTMFLRMGATE
                     QEVRTLRGIVAALVKGKGAGRKVP"
     assembly_gap    14305..14314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14315..15180)
                     /locus_tag="PK264_06245"
     CDS             complement(<14315..15180)
                     /locus_tag="PK264_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD75526.1"
                     /translation="MAISVDREGSAGAEPVCPDAKASVAEIVRVCHDQGVDRALARWP
                     SLTRDDLEPVLTYCAEQRCERDKAWCSTCRMRTLRDGLETFDAFVDRHSVISFRDTSV
                     TLTGRGTIQREEDSLDSLADRWRGEEFWFLARRVLRKLRHGPRRARELVAAIEHVGDT
                     PAIVLMEPQMAANVGMVARAMANFGLDELIVINPRDGWPSEKARILASGASAIVDGAV
                     VASTLKEGMGPLNWICATTARQRELVKPVLSPEEAAKEMRRRIAEGQRCGILFGRERN
                     GLTSDELANADAL"
     gene            complement(15199..15801)
                     /locus_tag="PK264_06250"
     CDS             complement(15199..15801)
                     /locus_tag="PK264_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD75527.1"
                     /translation="MAKSTDVMLERRPSAALIMKAIDDKALVIASLGTPNGDVAGAKD
                     RPLNYYLRGAMGCGSSLALGLALAQPERRVIAFLGDAELLMNAGSLATIGVMRPKNLS
                     IVVMDNERFGETGLQMSHTSFGIDIAMMAKGAGFLVAATVRTTTELERAVTAITTGTG
                     PSIHVIKVKAEKPGRVKLPYDAVWGKNRFREALLGPDGIV"
     gene            complement(15810..16322)
                     /locus_tag="PK264_06255"
     CDS             complement(15810..16322)
                     /locus_tag="PK264_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD75528.1"
                     /translation="MKDWRDEIHEVLSEAGIRQVAYLPDAGHSKLLELCEKDNRMRMI
                     PMANEFEAIGIAAGAWLGGEKSVVLIQSSGVGNCINALSLSAVAQYPLLMIVAMRGEF
                     GEGQRWQIPMGQAVRPTLEAMGVICYEARHADEVRSIVDNAATFAFQAHNRVAILLSQ
                     QLIGAKKFTN"
     gene            complement(16465..16977)
                     /locus_tag="PK264_06260"
     CDS             complement(16465..16977)
                     /locus_tag="PK264_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HRD75529.1"
                     /translation="MSRISAAFAAIAISSSALPAAKADDIVDTAVKAGSFKTLVAAAG
                     AAGLAPTLKGKGPLTVFAPTDDAFAKLPKGTVESLLKPENKKKLAAILAYHVVPGRYD
                     AARITKSKSKHFTLKTAGGAHLPVNLSKGVVVGGANVIKTDIAASNGIIHVIDTVMLP
                     PTPKKAKKHH"
     gene            complement(17181..18449)
                     /gene="ugpB"
                     /locus_tag="PK264_06265"
     CDS             complement(17181..18449)
                     /gene="ugpB"
                     /locus_tag="PK264_06265"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HRD75530.1"
                     /translation="MATPAAAQTEIHWWHALTGANNDVVVKLSNDFNASQKEFKVVPV
                     YKGNYADTMNAGIAAFRAGKAPHILQVFEVGTATMMAATGAVKPVHVLMKESGETFDP
                     KAYLPTVTGYYSTTKGEMLSMPFNSSTAIMWYNKDSFKKAGLDPAKPPVTWPEVFEAA
                     KKLQANGHPECGFSSAWITWLMIEQFSAWHNLPLATKSNGMDGFDTVLNFNSPLHTKH
                     LETLVELRKSKVFSYSGRTNQGEGRFTSGECPIFFTSSAFYGNVKANAKFDFGMAPMP
                     YYPGEGRPQNSIIGGASLWVMGGKSAAEYKGIAKFFTFLSDTKRQQWLHEVSGYLPIT
                     VAAYEATKASGFYGANPGREVPLIQMTGRAPTEHSRGRRLGNMVQLRDVWAAEIEAAL
                     DGKKTAAEALKAAIEAGNAILRQFERQVSR"
     gene            complement(18611..19468)
                     /locus_tag="PK264_06270"
     CDS             complement(18611..19468)
                     /locus_tag="PK264_06270"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD75531.1"
                     /translation="MTNVKVTLLSDVLGARIEGVDIARISDAEFADIERALHEHLVVV
                     IPRQSLEPAAFAAFARRFGKAEPHVIDQFHHKADPNILILSNRRNDKGEPIGLADGGT
                     YFHCDYSYLDVPARCTILYALEIPGSDAGTTFANQRRAYETLPADERAKLDGLVCRHH
                     YGNRDNLDEESRTAASRLSGEQKARMSWVRHPLVRKHPHGPWKSIYAVSGSSFGVEGM
                     DDAAGVALLNRLKQHSTAARYCYTPTYAPGDVVIWDNVSLLHSAPLIDQARPRTLWRI
                     TVKEAGPTI"
     gene            complement(19471..>20311)
                     /locus_tag="PK264_06275"
     CDS             complement(19471..>20311)
                     /locus_tag="PK264_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75532.1"
                     /translation="SHSPALIWIESPTNPLMTVVDLEALAKIGQANNILTVVDNTFLS
                     PYLQNPLDFGIDIVHGWGMTELSPVGTVTRLGPAEEVLLFDTWLDLKSRQGRRMYGVE
                     LKIVDADGKRLPHDGTAVGELFVRGPAVVKGYFNNPEASRKALDAEGWFGTGDVARIT
                     PDGFLIVVDRTKDLVKSGGEWISSIDVENVAIGAPGIANCALVGVPHPKWAERPLLVA
                     VKAPGAAPTRESVIEFLSTRIAKWQLPDDVVFVDALPMTATGKISKKDLRVKFADHKL
                     PGV"
BASE COUNT         3447 a         6595 c         6620 g         3639 t
ORIGIN      
        1 cctgcccccg ttccccgcct tcggacccgc catgctcctc ctgatcgaca attacgacag
       61 cttcacctac aacctcgtgc attatttcgg cgaacttggc gccaaagtgg tggtgcgtcg
      121 caacgatgcg ctggacgtac agcaggcgat ggcgatgcgg ccaacggcga ggaagtgttc
      181 cgcaagtgcc gctcgtgcca tgaggtcggc cccgatgcca agacgcggct cgggccgaca
      241 ttgaacggtc tcttcggtcg caaggccggc tcgatcgaag gcttcaagta ctctcccggc
      301 aacctcgagg ccggctcaaa gggcctcgtt tggaccgagg agacgctctt caagtacctc
      361 gaggatccac gcgctttcgt tccggggacc acgatggcat tcgcgggcct tgcagacgag
      421 caagatcgac gcgacgtcat tgcctacctg aaacaattca gcaagaaatg atgtggtttt
      481 tccgtgcgct tcgacgtgcg tgggtgaaaa ccatctcgac gagacttggc gtgccggcgg
      541 tgcttgcggc atactgacga ggttgccgcg catggcgcgc catgttccgt gaagtgcgac
      601 catggccagc tgcgtcatgc gcaaccgggc cctcacgtgt gcgaggagct ctgcatagcg
      661 atggggccag cgtgtccatg agatccagac tgatgggcct gctcgagaag atcggcctgc
      721 tctcactggc actgctgatt gcgatgggca ttggcggcac tcggctcgcg caatcgcagt
      781 cgagtgatgc cgacgagatc aaggtgctac agtatctgct gctgtggagc ggcgactacg
      841 ccggacagat cgatggtttc gtcgatccca tgctgcagca ggcagtccgc gacttccagg
      901 cccgcgcggg ggtgcgcgac aaggccttcg acagcgggca ggccaagcag ctcgtcgacg
      961 aggcaaccca ggccgccaag cgcgtcggct acgcctacat tctcgacgaa gcgacaaaag
     1021 cgcacgttgc gctgccgcgc gacatcctga gtgacggcaa ggccaacggt aaggggagcc
     1081 gcttcacgac gacggacaac ctcgtcgaag tcgagaccat ccgctacgag cccggcggcg
     1141 acgtcacgct gctgcagctc taccggaaat tcctcgagcc gctcgaccgg agcgcgctca
     1201 tccagaactc gttccgcggc gatcacttcg tgctggcctg gttcgtcggc agtcgccagc
     1261 acctcgtgcg catgcacgac cgcagcggcg aggtgcgcgg catcgaaatc cgcttcgatc
     1321 gcaacgcgcg gtccgcgttc tacccgatcg cggtcgccat ggtgcacgac tactcgccgt
     1381 tcgccccaca gtcggccgtg gccgcccttc gccagccgaa gatcacgagc acgctcgcgc
     1441 cctcggatga tccgccgcct cgcggcgaac cacgccgtgg ccggccgggt cctggcaata
     1501 cgggcccgat cggcccgagc ccgcgccgcg gctccgtgtc gatctcatcg ggcagcggct
     1561 tcattgtctc gccgcgcggc catatcctca ccaacgccca cgtggtggat gattgcacct
     1621 ccatttcgac aggcatccac aagagcgtga agctcgtcgg catagacaag cgcaacgacc
     1681 tcgcactgct gctgccgcct ggcacgccgc cgccgaacat cgtcgtcgcc aaattcgcga
     1741 gcgaggcggt gcggctcggc gaggacgtgg cgaccttcgg cttcccactg cgtgatatcc
     1801 tagccgatca actcaacatg acgacgggca tcgtcagctc gctcgcgggc ctcaggggtg
     1861 atccgaaatt catccagatc tcggcgcccg tccagcgcgg caactcgggc ggaccggtcg
     1921 tcgacctcgc cggtcgtgtg atcggcgtcg tgacggccaa gctcgacgca ctcggaatag
     1981 ccaaggcgtc gggcggcgac ctgccgcagg tcgtgaactt cgccgtgcgc catgaactcg
     2041 gcatcagctt catgcgccgc tacggggtcg agccgatcct tgcgccggag actgaggcga
     2101 tgcgcaagcg cgaccttgcc aagctcgcgg cggacttcac gctggcgatc acctgcgaga
     2161 agcgctgacg cgccagatcg agtgcacagc tgtcgttgga ttgccgtcgc ggtgtcgact
     2221 gcagtcgtgc gggcatcgcc gcacaagcgg acccgttgac gcctttcacg tttgatttcc
     2281 aagtgtttcg acattgtgat cgatcggcca agcaatcgcc cggcgctcgt cacgctttgg
     2341 cctcgatccg acggccaaat gttaactgtc ttacgcagcg ggaacttaaa gaagaggtcc
     2401 cgacaactta cgaagctggt gcgctgaact taccagcctt cagtacaggg cctgcggacc
     2461 ttgaaaataa actcgggtcg cgcaacaaga tgctcgcccg acgcgtctga gatcgcggcg
     2521 gcgagcggcg ggctccgccg gaacgacatc cggcgcgatg gaagccccag agagctaccg
     2581 tcgcgatcag ctgtcgagag tgcagccgcg acaggtgttg gcagacgcga tcggaacgcc
     2641 agggcacgca aatcgtgcac caccgagatg cgactgcccg gtcggccatg gacatgagcc
     2701 attcatgaat atggctgacc ggtgacggcg acaccccgcg ttgccgtcac gaatttcaag
     2761 cccatcgatc ggcagggcga tgtgtccgac ccgatcggtg gccgagaagc cggaaggccc
     2821 gcgacctgcc gcaccgccac acctccctcc ccgacgcggc agtcgcggtc cttccggcgg
     2881 acataccgcc ccgaaaccca acggccccaa agcccccaag atgtgggcgc atcggacacc
     2941 ctgagcctgt tccttcgtgc tcgactgagg cgccctcgac gccatatgct cgctctctcg
     3001 gggagccgca gcgatggccg tgtcacccga cccgtccagc cttgcgacga gcggagcgac
     3061 agtgagcctg ccgaatatta tcacgctgtg ccggatcggc ctcgttccag taattttctg
     3121 ggcattcgtc agcggccggc cggtcctggc gttcgcggcc ttcgtcgtgg ccgggatttc
     3181 cgatgccgtc gacggcttcc tcgccaagac gttcgacatg aagacagagc ttggcgccta
     3241 tctcgatccg attgccgaca agctgctgat cgtcagcatc tatgtggctc tcggcgtcgt
     3301 gaaggaactg ccactgctcc tcgtcgtcct tgtcgtcagc agggacatcc tgatcgtgct
     3361 cggcgtactg ctcgcctggc tcctcgaccg gccggtcagg atcgcgccgt ccgaggtcag
     3421 ccgctggaat acggcggcgc agctgctgct ggtcgcaacc gtgctcgccg acgaagcttt
     3481 caagcttcag ctcgaggcgc cctggatcgg aggggcctcg ctcggctatt gttcggtccg
     3541 cgacattctg gtgtggagca cggggatgct caccatactt tcgctcggcg actatgcgcg
     3601 gatctggctg cgtcacatgt ccggggaagc gggagcagcc ggctgaattg cgcccgggcg
     3661 cgggcgcaca tgagacgggc aaagccgcgc gacggcatga gcagcgggga gagactgatg
     3721 cgcatcgaca ggcaggccct cttctggacg gcggcactcg gcgtggtact cgtgctgctc
     3781 tccatgctga aaggcatgct gctgccgttc gttgcaggct tcgtgctcgc ctatttcctc
     3841 aatcccgccg tcgatgcgct ggagcgggcg ggcctcggcc gcacgctcgc ctcgagcctt
     3901 atggtggtcg cgggcgtatt gctgatcgcg gccatgatcc tggtgctcgc accgatcctc
     3961 gccgaacatg tccggctcct ctccgagacc cttccgggtg acctgcagcg cgcccgcgcc
     4021 acgatcgaga agttcctcgc cgatcggctc gggccgggct ttgcctctat tgcggccggc
     4081 atcgacaagg ccatggccgc gctcgaggcg aacagctcgg gcctgctctc gaccgccgcg
     4141 caggcgatct ggagccgggg gcaggcgatc gtcagcatgg tgtcgctgct gctggtgacg
     4201 ccgctcgtcg tattctacct gctcgtcgac tggcaccgga tggcagatcg cgtcgacggc
     4261 tggctgcccc gcgagcatgc tcccgcgatc cgggcgatcg ccacggacgt taacgcggcg
     4321 atggcggcct tcatccgcgg gcaaggtctc atctgcgtca ttctgagcat cttctatgcc
     4381 atcgcattga cgctcgccgg catcccctat ggcggcatca ttggggtcac cacggggctc
     4441 ctctcgttca ttccgttcgt cggctggatg ctcgggctga tcgtcgccgc cggggttgct
     4501 ctcgcgaagc actggccggc tttgggtccg ctggtgctgg tcgtcgccat tttcgctgcc
     4561 ggcatgctgc tcgacagttt cctcttgtcg ccgcggcttg tcggcgagcg cctcggcctg
     4621 catccgctga ccctgctgtt cgcgctggcg gccttcagtt atctattcgg cacagtaggc
     4681 ctgatgattg cggtgccgct gtcggcagcg ttcgtggtcg tcgcgcggca tggcctcaag
     4741 gcctatctcg cgagtgacgt ctttcaggga gaggcattgc cggcgcccct tgcgcggccg
     4801 acgacgccgg cagccggcgc tctcccggtg cgcaatgcct cgtcgcgagg cgagatgtca
     4861 tgagcgcccc gcaccagctt gtcctcgatc tcgggcaccg gccggcgctc ggggccgagg
     4921 attttcacgt gaccgcgtcg aacgagacgg caatcgcgct cgtcgatcgc tggcccaact
     4981 ggccggggcc ggccgtctac atccagggtc cggcaaagtc cggaaagacc cacctcgtga
     5041 acgtgtggcg ccacatgtcg cacgccccgg tccaggcggc tgaggctctc gacgatacgg
     5101 caataccttt gctgctcgag cagaaggcgc tggcggtcga ggacatcgat cgcggcatcg
     5161 cgagcgagcg ctcgctgttc cacctcctca acgcggcccg tgagaacaac gcgcgactgc
     5221 tgctcaccgg ccgccggccg acgggtgaga tcgagattgc cctgcccgac ctccggtcac
     5281 ggctccgcgc gctgccgatt gccgtcatag agccaccgga cgacgcattg ctggcagccc
     5341 tcctcgtcaa gcatttcagc gaccgccagc tgctggtcga tccgccggtg atcgcctatc
     5401 tgacccagcg catggagcgc tctgccgccg cggccgcggc actggtcgag gcgatcgacc
     5461 ggcgcgcgct cgcgacgcac cgccgcgtca cgcgcgccct ggcctcggaa gtgatcctgg
     5521 cgcgcgaggt cggcatgtca cccgagtgaa aaattgccgt gctcagaggg tgcgtgcgcc
     5581 tttggctgta acctcgcgag cgagccttcg ggctgatgga gcctttgaat gtcgatgatc
     5641 aaggacgatg ctggcaaggc cgggcaggag cggtcgcccc gtagcgcaac tgctgccgac
     5701 cgctactaca atcgcgagtt gtcatggctt cagttcaatc gacgcgtgct cgaggaggcc
     5761 ctgaaccttc gccatccggt gctcgagcgg ttacgcttcc tcgccatctc ggcatcgaac
     5821 ctcgatgagt tctatatggt ccgggtggcc ggactgatcg ggcagatccg cgccggcgtg
     5881 ccgacgctga gccaggacgg cctcaccgcc tccgagcagc tggccggcgt caaccgcttc
     5941 gtcgccgatc tcgtttcgga gcaccaggcg tgctggacct cgcttgccgc ggagatggcc
     6001 aaggccggga tcgagatcgt cgagccggga tccctggtcg gcttcgagcg ggactggctc
     6061 gagagcgagt tcatcgaccg catgttcccg atcctgacgc cgatcgccgt ggacccggcc
     6121 caccccttcc cgttcattcc gaacaagggg ctgacgatcg gcgtcgaact gcagcgcgat
     6181 gccgacgggc gcacgatgca tgcgcttcta ccgatcccat ctcagctcgc ccgcttcgtc
     6241 aggctgccgg ctgcgcagcc gccgaagggc gagcgcgccc gcgcccgttt catcgccatc
     6301 gagaccttca tcggcatgtt tctcggccgc ctcttcccgc gcttcacggc gaagaaccag
     6361 ggcgcctttc gggtgctgcg cgacagcgat ctcggcatgc aggaggaggc ggaagacctc
     6421 gtccgttcgt tcgaatcggc actgaaacgg cgacagcggg ggcacgcaat ccgcgtcgag
     6481 ctcgacggcc gcatgcccgc gccgctgcag cgcttcgtcg tcgaggagac cgaggtcggc
     6541 gaggcgggcg tgttcgtgaa ggagggcatg ctcggcctcg tcgacacgac agagctgatc
     6601 gtggcggatc ggcccgacct tatgttcaag ccgttcacga tccgatttcc ggagcggatc
     6661 agggcgctcg gcggcgattg cctcgcggcg atccggcgga aggacatcgt tgtccaccac
     6721 ccctatgaat cgttcgacgt cgtcgtccag ttcctgcgcc aggcggttgc cgatcccaac
     6781 gtgatggcga tcaaatggac gctctaccgc acctcgcgtg acagcccgat cgtgcaggcg
     6841 ctcaaggagg cggtcgaggc ggggaagagc gtgacggcgg tggtggagct caaggcgcgc
     6901 ttcgacgagg ccgcgaacat ccgctgggcg cgcgacctcg agaacgcggg cgtgcacgtc
     6961 gtctacggct tcgtcgagct gaagacgcac gccaagctcg ggctcatcgt gcgccgcgaa
     7021 ggcagcgagc tcgcaaccta cagccacatc ggcaccggca actatcaccc gcagacggcg
     7081 cgtgtttaca cggacctgtc gttcttcacg gcggacaagg ccatcgggcg cgatgtgaca
     7141 cgcatcctga acttcgtcac gggctatgcg gagccgaccg agctcgaggt gatgtcggcg
     7201 agccccaagg gcatccggga gaaaatctct gctcacatcg atggcgagat cgcccacgcc
     7261 aaggccggtc gaccggccgc gatctggatg aagatgaact ccctcgtcga ccagcgcatc
     7321 atcgatcagc tctatgctgc gagtgcggcc ggcgtgccgg tggacctgat cgtgcgaggc
     7381 atttgctgcc tccggcctgg cattccgggg ctttccgaca acatccgtgt gaagagcatc
     7441 atcggccgct tcctcgagca tgcgcgcatc tactgcttcg gcaatggcca tggcctgccg
     7501 tctcccaagg ccgccgtcta catcagctcg gcggacatga tgccgcgaaa ccttgaccgg
     7561 cgggtcgagg ctatggttcc gctgagcaac aagaccgttc atgcgcaggt gctcgagcag
     7621 atcatggtgg caaacctcaa ggacaaccag cagagctggc aagtgctggg tgacggctcg
     7681 tctcggcgcc tgacggcggg cgatggcgag aggccgttca acgcacacga gtatttcatg
     7741 accaatccga gcctgtcggg tcggggcgac gcgctgaaga cgcatggtcc accccgggta
     7801 aagatcgaga gccgccgcgt cgcgagcaag aagagcgggt gaggcgtgtc gtcaggcaat
     7861 gatcgcggtc gattttccga gctcgagccg ctgtcggtgg tcgacatcgg ctccaactcg
     7921 gtgcgcctcg tggtctacga gggcgcggtt cgcacgccgg tcccgctgtt caacgagaaa
     7981 gtgcattgcg ggctcggccg ggccgtcgca tctaccggtc ggctcggtga tgatgcggtt
     8041 cagcgcgcgc ttgacgccct gacacgcttc cgctcgatca accgcatcct gaaggtgaag
     8101 aacatccgcg cgatcgccac ggcagcggtc cgcgatgcca gcaacggcgc cgagttcatc
     8161 cgccaaggcg agcgcaatct cgggctcaag atcgacgttc tgtccgggct cgaggaggcc
     8221 gagctcgcgg cacttggagt gcacatgggc tttgccaacc cggacggcgt gacgggggat
     8281 ctcggcggcg gcagcctcga gctcatcgat gtgctcgatc cgaagggtcg ggtgcccgtt
     8341 tcgctgccgc tcggaggcct ccgcctcatc gatgcgtccg gcggcagcct cgaacaggcg
     8401 atgaagatca cggacgagca gcttggcggc gtctcctggc tgaagcaggg agaggggcgc
     8461 accttctacg cggtcggcgg atcctggcgc gcgctcgctc gcctgcacat ggacgagcac
     8521 gactacccac tgcgcgtgat gcacggctat cagatccagg cgcgcgaggc gctcgagttc
     8581 tgcgagaggg tccgcaaggc caaaaagatc agcgatctca agggtgcggc gaccgtatcc
     8641 aaggcgcgcc gcgaggtgct gccgcagggt gccctggtgc tcgagcgcat cgtcaagcgc
     8701 atgaagccga aggatgtggt attctcagtg ttcggcatcc gcgaaggcct gctctatcag
     8761 ctgctcggcc cgacggagcg cgcgcgcgat ccgctgctcg ccttctgcga ggactatgcg
     8821 cggctgcgct cgcgctcggt agagcatgcg cgcgagctga tcgcctggac ggaccagatt
     8881 ttccgcgatc ccggacccgg cgagaccgag gacgagcgcc ggttccgcca tgccgcctgc
     8941 ctcatctcgg acatcggttg gcgcgcgcat cccgactacc gcggcgagca gagcctcaac
     9001 gtgatcgcgc acgcggggct cagcggcatc gatcatccgg gccgcgtttt cctcgcgctg
     9061 acggtctact accgccatgt gggcccgagc ggcgaggacg gcggccacga cgacttctcg
     9121 gaacgcatga aggacatcgc gccgaagcgg ctcgtccgcc gggcacgcat cctcggcgcg
     9181 gcgatccgca cggcgcacat gctctcgatc ggcatgccag ggatcatcga cgagacgcgt
     9241 atcggctacg cccgggacaa gctcgtcctc aaggttccgc acgcctacca gagtctcaac
     9301 ggagagcgcc tgcaacgtcg attgcaggcg ctcgcgacgg agctcgaaaa gcgcgccgag
     9361 atcgagatcg tcgcgtgatc ccggccaact gatcctttga cttgagaact ctcgtgcagt
     9421 tggccggtat cgatcctttg atgtgcgcgg ccgctccacc aaccccgcac gatactccgc
     9481 caccaatggt caagcttttc gtagctccgt atgacgcgtg acgtgcacag gggtcacgct
     9541 ccgccgacgg tctatcgtgc gcggttcgaa gaaatgtgcc atcatgtcga cacagcatga
     9601 cccgatgtgg tctcgcgcgt gagctgcctt cgcgccgtga cgcctggggc acgaaaagtc
     9661 ggggttctgc tgccgccccg aatctccctt gttggctggg gcgccatggc cggcttcagg
     9721 cgtctgccgg aagtcctgac atcggagttc cggtgacggc tctggcgaca gcgacatctg
     9781 ggcagtgcgc ttgcgtctgg gctggtgcag cgcggtcgag gcatcgagga cggtagagga
     9841 taggatcgtg accgttatgg ccgacgaccc gtacaaggtt cttggcgtag cgcgggatgc
     9901 gtccgaggac gatatccgcc gcgcctatcg caagcttgcc aaggagctgc atcccgacct
     9961 caatccgaac aaccgggcaa gcgctgagga gcgcttcaag aggatctcgg ccgcctacga
    10021 catcgtcggc gatcctgaca agcgcgcgaa gttcgaccgc ggcgagatcg acggcaacgg
    10081 cgacgcgcgc cgtggctatc agcgcgccta tgcgggccga ggcgggaccg gcggagcgcg
    10141 ggcggacgac ctcggatttt cgtccatctt cgaggatctg ttcggcgggc gaaccggccc
    10201 gacgcgcgcc cgcggcggct tctcggtccg cgggcaggat gtgcgctact ccctcgaagt
    10261 cgactttctc gaagcggtgt cgggtgcgcg caagcgcgtg acgctccccg acggcggcac
    10321 cctcgatctt tccgtgccgg agggcgtcga gaacgggcag gtcctccgcc tcaagggcaa
    10381 gggcgcgcag ggcgtgaacg ggggcgatgc cggcgatgct ctggtcgaaa tcaaggtgcg
    10441 cccgcatgcc cagttccgcc gcgcgggcga cgacatcgcc ctcgagctgc cgattaccat
    10501 cgacgaagcg gtgctcggcg ccaagatcga ggtgccgacg gcgaccggcc gcgtctcgct
    10561 gacgctgccc aagggcacaa gctcgggccg cgtgttccgc ctcaagggca agggtgtccg
    10621 caacctggcg acaggcgtgc tcggcgacga gctcgtcacc atccgcatcg tcatgccgga
    10681 gtcgatcgac gataatctcg cgttcttcat gtcggagtgg cgccagaaga acgcctacga
    10741 tccggggcgc aagtaggcgc cgcgcctgtc gaactgcact atggtgctct cgacggccat
    10801 cggccaatgt cggatagtgc aaaattgaac acagtcctgg tggattgatc tgcgctccgg
    10861 cggcggatgc gtgccagcaa ccgtgtttcg gaatgagaac cgtaaaggga ggagacacca
    10921 atgacagcag gcaaggatgc gggtgccgca aagctctcgc ggcgaaccct tatcaaggca
    10981 gcctcggccg cggcagcggc ttcgaccgta ccgaccggct gggcgatcgc gcaaggcaag
    11041 cccctcaaga tcggcctgat gctgccttac tcgggcgcat tcgccaagct cggcgaaaac
    11101 atccacacgg ccatcgagct gctgatcgca gagaagggcg gcaagctcgg cgggcgcacc
    11161 atccagatcg tcaagctcga cgacgaatca aagcccgatc ttgcggcaca gaacgcggag
    11221 cgcctcgtca agcgtgaggc cgtcgacgtg ctgatcggca cagtgcactc gggcgtgcag
    11281 ctcggcatcc acaaggtcgt caaggagagc ggcacactca ccatcgtgcc gaatgccggc
    11341 aacggggccg tgacacgcaa cgagtgcgcg aagaacgtgt tccgcatgtc gttctcgaac
    11401 tggcagcccg cgcacggcat ggggctccac cttggcaaga agggcatcaa gaaggccgcc
    11461 tggatcactt gggactatgc ggccggcacc gagagccgcg acggcttcaa ggacggtctc
    11521 gagaagggcg gcggccaact catgcagacg ctgacgctga agttccccga gacgaacttc
    11581 cagccgctgc tcgcccagat cgccggcctc ggcgtcgagg cggtcggcgc gttcttcgcg
    11641 ggtggccccg cggcccagtt cgtcaaggaa tacaaggccg ccaacatctc ggtgccgctc
    11701 tgcggctcgg gcttcctgac cgagggcgtg ctcggcgcgg cgggcccggc ggccgagggc
    11761 atcgagaccg cgctccacta cggcgacggg ctcgacaacg ccaagaacgc ggcgttccgc
    11821 aaggccttca aggagaaggc aggacgcgac gccgacgtct acgccgtcca gggctatgac
    11881 agcgcgcagc tgctcgccgt cggcctcgag gccgtcaagg gcaacatgga cgacgaggcc
    11941 ggcctctaca aggccatgcg cgccgccaag atcgagagcc cgcgcggccc cgtctccatc
    12001 tcggccgccc agaacgtggt gcacaacatc tacctccgga aggtggtcgg tgccgagaac
    12061 aagatcatcg gcgtcgcggc agagaacctc gccgaccccg gcacgggctg cacgctgaag
    12121 tgatgtcgca actgcgcggg ctgggcggcg gcatgaccgc agcccagccc gcgtcacgtt
    12181 cgagcatcgc atgcatgcga ggatcaatcg cagatgcatc cggtcgacat cgatggtcac
    12241 tcgatcgagg tacgcaccat tccgggcgac gcgtccctgg cgacgctggt tttcctgcac
    12301 gagggcctcg gctcggtcgc actctggcgc gattttccgg acaaggtctc aaggcagctg
    12361 cgcgcgccgg cggtcatcta ctcgcgcgtg ggctacgggc aatcgagcgg cctctgcggc
    12421 ccgcgcagcc ccgacttcat gcatcgggaa gcgctcgacg tgctcccgaa gctgctcgac
    12481 cgacttggca tcacgcgccc gctgctgatc ggccactccg acggcgcctc gatcgcgatc
    12541 attcatgccg cgtcctcggg acgcgaggtt gcaggcgcgg ttctcatggc cccgcacgtg
    12601 ctcgtggagc cggtctgcac cgaggcgatc gggcgggcgc tcgcgaccta ccagcggggt
    12661 gacctccgtc agcgcctcgc gcgctaccac cgtgacgtgg acgatgcgtt tctgggatgg
    12721 gcgcgaatct ggctatcgcc gaccttcaag gactggtcgc ttcggtccga gtgcgggcac
    12781 cttgccgtgc cgctgctgct gatccagggg cggcaggacg aatacggcag cttgctccag
    12841 ctcgacgtca tcgaggatga ggtgcgccgc ggcggtgttg cggcaggccg gccggttccc
    12901 gagatccagc gcgtcgaact cgacgactgt cgtcattcgc cgcatcggga ccaggaggcg
    12961 cgcgtcatcg ccgagatagc aaaatggtcc gcgggccttc gccagcggac cacctgatga
    13021 cgtcgtgacg atctgcttca aggcctggag cgacgccgga ccaataaatc acttcttcgg
    13081 cggttgcggc tgagaaacgg gaacaggacc gctcgcagct ggcggcggtg tcgccgcggt
    13141 agccggcgga ggcgtcgtgc cgggcgcggg cggtgtcgcg atgccgccgt gcttggcgat
    13201 gtaggtgagg atctcggact tgatcttctg gcgcttggcc tcgtcgcagg cggccttctc
    13261 gatcggctcc aggatgacct gctgctcgtc cgggcccttc tcgacgatgc gcaccttgcc
    13321 ggcggcgaag tgaatggtca tgcggtgcag ggtcgagatg tagaggagtt gctgctccgt
    13381 ccacttcttc ttcgccactt cgagccgcat catggtgtga tagttggcgt tctgctcctc
    13441 ccacagctcg cagagctggg cgtgggcgga gttgtagccg accaggatga cctcgcgggc
    13501 cacgtcgagc ggaacgacgt tttcctcgcg cttcttcttg tcgatctcga tcttcttgcc
    13561 agtcgtggac gtgaactggg tcggcaggat ctgccaagca tagttcatca aggccatgac
    13621 tgtcttgtcg ctgatcttcg gcttctcctg ctgtgctgcc acgccgaaaa tcgatgcaaa
    13681 catgagagtg ctggcgccga gcgcagccac ccctgcattg aagagccacc tgctcattgt
    13741 cgctgcccct gtcgcctagt cgcctcgccg gcccaaacgg tcggcatgat gatgtcgatc
    13801 tttgtccgaa gatcgacgtc caaagagagc catgccggca ttctcgactt atggttgttg
    13861 agtccggttg gcttgcccac cttcccgggc acgcgcatcg ctcgttccaa agtgaggcaa
    13921 gtttaaggca ccttgcggcc tgctcccttg cctttgacaa gtgctgcgac aatccctcgc
    13981 agagttctca cctcctgctc tgtcgcaccc atgcgcagaa acatcgtgcg gatgtttcgc
    14041 accattgtct gtcgtttgtg gggcgggaac aaaaagccca tccggtcgag ctcggtttcg
    14101 aggtgctcga agaatcccgt catttcctcg cggctcgcga gccgcccctt cttgatccgc
    14161 agcccctcct cgaccggatc ctcgtaggtc gtgacgcggc cgagcgtggc ggcgccggtc
    14221 tgcttgagcc attcgtagcc gagaatgagc accgcttggg cgagattgag tgaggcgaaa
    14281 ctcgagttca ccgggatcat gacgnnnnnn nnnnagcgcg tcggcgttgg cgagttcgtc
    14341 cgaggtgagg ccattgcgct cgcggccgaa caggataccg cagcgctggc cctcagcgat
    14401 gcgccgacgc atctccttgg cggcttcttc gggcgacagc acgggcttca cgagctcgcg
    14461 ctggcgggct gtcgtggcgc aaatccagtt gaggggaccc ataccctcct tgagcgtcga
    14521 tgcgacgacg gcgccatcga cgatcgcgga ggcgccggat gcgaggatgc gcgccttctc
    14581 cgaaggccag ccatcgcgtg gattgatgac gatgagttca tcgaggccga aattcgccat
    14641 ggctcgggcg accatgccaa catttgccgc catttgcggt tccatcagca caatcgctgg
    14701 cgtgtcgccg acatgctcga tcgccgcgac aagctcgcgg gcgcgacggg ggccatgccg
    14761 gagcttgcgc aggacgcggc gggcgaggaa ccagaactcc tcgccgcgcc agcggtcggc
    14821 gagtgaatcg agactatcct cctcgcgttg gatcgtgcct cgccccgtga gcgtgacaga
    14881 tgtgtcgcga aacgagatga ccgaatgtcg atcgacaaag gcatcgaacg tctcgaggcc
    14941 gtcgcggagc gtgcgcattc ggcaggtcga gcaccacgcc ttgtcgcgct cgcaacgctg
    15001 ctcggcgcaa taggtgagaa ccggctcgag gtcgtcccgc gtgagcgacg gccagcgcgc
    15061 gagtgcccgg tcgacgccct gatcgtggca aacgcgcacg atctccgcga ccgatgcctt
    15121 ggcatcaggg caaacgggtt cagcacccgc cgatccctcc cgatcgactg agatcgccac
    15181 gcgaggctcc gcttgccgtc aaacgatgcc gtcgggaccg agaagcgcct cgcggaatcg
    15241 gttcttgccc cacacggcat catagggaag cttcacgcgg ccgggcttct cggctttcac
    15301 cttgatcacg tggatcgagg ggcccgtgcc ggtggtgatc gctgtcaccg cacgctcgag
    15361 ctcggtcgtt gtgcgcaccg ttgccgcaac cagaaagccg gcgcccttcg ccatcatggc
    15421 gatgtcgatg ccgaacgagg tgtgcgacat ctgcagcccg gtttcgccga aacgctcgtt
    15481 gtccatgacg acgatcgaga ggttcttcgg gcgcatcacg ccgatcgtcg ccagcgagcc
    15541 cgcgttcatg aggagctcag catcgccgag aaaggcgatc acgcgccgct cgggttgagc
    15601 aagggcgagg ccgagagcga ggctcgaacc gcagcccatg gcgccgcgca ggtagtaatt
    15661 gagcggtcgg tccttggcgc ccgcgacatc gccgttgggc gtgccgaggc tcgcgatcac
    15721 cagcgccttg tcgtcgatgg ccttcatgat gagcgcggca gaggggcggc gctccagcat
    15781 cacgtctgtc gattttgcca tgaccgacct caattcgtga acttcttggc gccgatcagt
    15841 tgctgcgaga gcaggatcgc gacgcggttg tgggcctgga atgcgaaggt cgccgcatta
    15901 tcgacgatgg agcgcacctc gtcggcgtgg cgcgcctcgt agcagatgac acccatcgcc
    15961 tcgagcgtcg gccgtacggc ctggcccatc ggaatctgcc agcgctggcc ctcgccgaac
    16021 tcgccgcgca tggcgacgat catcagcagc ggatactgcg cgaccgccga cagggagagc
    16081 gcgttgatgc agttgccgac gcccgacgac tgaatgagga cgacgctctt ttcgccgccg
    16141 agccaggcgc ctgccgcgat gccgatggcc tcgaactcgt tggccatcgg gatcatgcgc
    16201 atgcggttgt ccttctcgca aagctcgaga agcttggagt gtcccgcatc cggcagatag
    16261 gcgacctgcc ggatgccggc ctccgagagg acttcgtgga tctcgtcacg ccagtctttc
    16321 atcgcccggg catctccata ttcgatctcg ggccgacctt agggcctctc cagaaaaaat
    16381 gcatcgtcaa accgcgcgaa ggactgagac aagcgaatgc gctcccgaca aggccgggag
    16441 cgcttcacct ggaccgcagg cgcgtcagtg atgcttcttc gccttcttgg gcgtcggcgg
    16501 cagcatcacg gtgtcgatca catggatgat gccgttcgag gccgcgatat ccgtcttgat
    16561 gacgttagcc ccgccgacga cgacaccttt gctaagattg accggaaggt gtgcgccgcc
    16621 cgcggtcttg agcgtgaagt gcttcgattt cgacttggtg atgcgcgccg catcatagcg
    16681 gccgggcacg acgtggtagg cgaggatcgc cgccagcttc ttcttgttct cgggtttgag
    16741 gaggctctcg acggtaccct tcggcaattt cgcgaaggcg tcgtcggtcg gggcgaagac
    16801 ggtgagcggg cctttgccct tgagcgtcgg cgcgaggccg gcggcgcccg cggcggctac
    16861 aagcgtcttg aacgatccgg ccttcacggc ggtatcgacg atgtcgtcgg ctttggcggc
    16921 cggcaacgcc gaagacgaaa tagcgattgc tgcgaacgct gccgagatac ggctcatgaa
    16981 gcgcatgtcg tttttccctg tttggtcgcc aacagagttg gcggctgaag acgatgagct
    17041 aacgactgtg caaccctcgc ggatcgccgc ccgcgaaccc aaaaatttca atgccggctg
    17101 tgatctgccg gcctgccaaa aacgttgggg ccgacttttc agccggcccc acagagttct
    17161 tgatatcgat acggcggccg ctaccggctc acctgacgct cgaactggcg caggatggca
    17221 ttgcccgcct cgatcgcggc cttaagggcc tcggccgccg tcttcttgcc gtcgagtgcc
    17281 gcctcgattt cggcggccca gacgtcgcgc agctgcacca tgttgccgag gcggcgacca
    17341 cggctatgtt ccgtcggcgc gcgacccgtc atctggatga gcggcacctc gcgacccggg
    17401 ttggcgccgt agaaccccga cgcctttgtc gcctcgtaag ccgcgaccgt gatcggcaag
    17461 tagcccgaga cttcgtgcaa ccactgctgg cgcttggtgt ccgagagaaa cgtgaagaac
    17521 ttggcgatgc ccttgtactc ggcggcactc ttgccgccca tgacccagag cgaagcgccg
    17581 ccgatgatcg agttctgcgg ccggccttca cccgggtagt agggcatcgg cgccatgccg
    17641 aaatcgaact tcgcattcgc cttcacattg ccgtagaagg ccgacgacgt gaagaagatc
    17701 gggcattcgc cggaggtaaa gcgaccctcg ccctgattgg tgcggcccga gtagctgaac
    17761 accttgctct tgcgcaactc gacgagcgtc tcgaggtgct tggtgtggag cggcgagttg
    17821 aaattcagga ccgtgtcgaa cccgtccatg ccgttcgact tggtggcgag cggcagattg
    17881 tgccaggccg agaactgctc gatcatcagc caggtgatcc aggccgacga gaagccgcat
    17941 tcgggatgac cgttggcctg cagcttcttc gccgcctcga agacctcggg ccaggtgacg
    18001 ggcggcttcg ccgggtcgag gcccgccttc ttgaagctgt ccttgttgta ccacatgatg
    18061 gccgtcgagg aattgaacgg catcgacagc atctcgccct tggtggtcga gtagtagccg
    18121 gtgaccgtcg gcagataggc cttcgggtcg aaggtttctc ctgattcctt catgaggacg
    18181 tgcacgggtt tcacggcgcc ggtcgccgcc atcatggtcg ctgtgccgac ctcgaacacc
    18241 tgcaggatat ggggcgcctt gccggcgcgg aacgcggcga tgccggcgtt catcgtgtca
    18301 gcgtagttgc ccttgtagac cgggacgacc ttgaattcct tctggctcgc gttgaagtcg
    18361 ttcgagagtt tgacgacaac gtcgttgttc gcgcccgtga gcgcgtgcca ccagtggatt
    18421 tcggtttggg cggcggcggg cgtcgccatt gcggctgcgg ctattccgac gcctgcgacg
    18481 atacccgcaa gcctgatcct gacaaatccc atgctctgcc tcccatgatt gcagcgtcgc
    18541 cgtctattcg cagttcattg tgacggtcgc ataacgcttt gcgccatgcg ttaccggagt
    18601 caatggcaca tcagattgtc ggcccggcct ccttcacagt gatgcgccag agcgtgcgcg
    18661 gcctcgcctg gtcgatcagg ggcgcggagt ggaggagcga gacgttgtcc cagatgacga
    18721 catcgcccgg cgcataggtc ggcgtgtagc agtagcgtgc cgcggtcgaa tgctgtttca
    18781 gccgattgag aagggcaact ccggccgcat cgtccatgcc ctcgacgccg aaggatgatc
    18841 ccgacaccgc atagatcgac ttccagggac catgcggatg cttgcgaacg agcgggtgac
    18901 ggacccagga catgcgcgcc ttctgctcgc cagagagcct ggaggcggcg gtacggctct
    18961 cctcgtcgag gttgtcgcgg ttgccgtagt gatggcggca gacgaggccg tcgagctttg
    19021 cgcgctcgtc cgccggaagt gtctcatagg cgcggcgctg attggcaaag gtcgtcccag
    19081 cgtcgcttcc cgggatctcg agcgcataaa ggatcgtgca gcgcgcgggc acgtcgaggt
    19141 agctgtagtc acagtggaaa taggtgccgc cgtcggcgag gccgatcggc tcgcccttgt
    19201 cgttccggcg gttcgagagg atcaggatgt tcggatcggc cttgtggtgg aactggtcga
    19261 tcacgtgcgg ctcggccttg ccgaagcggc gggcgaaagc ggcgaaggcc gcaggctcca
    19321 gcgactgcct cgggatgacc accacgagat gctcgtgcag cgcgcgctcg atatcggcga
    19381 actcggcgtc gctgatccgg gcgatgtcga caccctcgat gcgggcgccg agcacatctg
    19441 acaacagggt gaccttcacg ttggtcatac tcagactccc ggaagtttgt gatcggcgaa
    19501 tttcacacgc agatcctttt tcgagatctt gcctgtcgcc gtcatcggca gggcatcgac
    19561 gaacacgacg tcgtcgggga gctgccactt ggcaatgcgc gtcgacagaa attcgatgac
    19621 gctctcacgc gtcggcgcgg cacccggcgc cttgacggcg acgagcagcg gccgctcggc
    19681 ccatttcgga tgcggcacgc cgacgagcgc gcagttggcg atccccggcg cgccgatcgc
    19741 cacgttctcg acgtcgatcg aagagatcca ctcgccccct gacttcacga ggtccttggt
    19801 gcggtcgacg acaatcagga agccgtccgg ggtgatgcgg gcgacgtcgc cggtgccgaa
    19861 ccagccttcg gcatccagcg ctttccgact cgcttcgggg ttgttgaagt agcccttcac
    19921 gacggccggc ccgcgcacga acagctcgcc gaccgctgtg ccgtcatggg gcaggcgctt
    19981 cccatccgcg tcgacgatct tcagctcgac accgtacatg cgacgcccct gccgcgactt
    20041 gagatcgagc caggtgtcga acagcagcac ctcctcagcc ggtccgagcc gcgtcaccgt
    20101 gccgacgggt gacagctccg tcatgcccca gccgtgcacg atgtcgatgc cgaaatcgag
    20161 gggattttgc agatagggcg agaggaaggt attgtccacc acggtgagaa tgttgttggc
    20221 ctggccgatc ttggcgaggg cttcgagatc caccacggtc atcagggggt tcgtcggcga
    20281 ctcgatccag atgagggcgg gcgaatgcga g
//
LOCUS       DAORNH010000040        20238 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2061_length_20238_cov_2.596740, whole genome shotgun sequence.
ACCESSION   DAORNH010000040 DAORNH010000000
VERSION     DAORNH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20238
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2061_length_20238_cov_2.596740"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..678)
                     /locus_tag="PK264_06280"
     CDS             complement(205..678)
                     /locus_tag="PK264_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75533.1"
                     /translation="MSPIDQLKVKNYLAKDPMIWPFLEMALLKTREIERLCRQILIFE
                     QLSQLSHLPKGQRGEPQNRPKVSGRPRKTFQQAFYELADDPGRRQRLGCNPQRKNDWR
                     ARALKRGSFEDVAIAIGLAISIELSFSYEEDPNTFHDTAIDMATIKKMFRDAKRR"
     gene            complement(784..>1259)
                     /locus_tag="PK264_06285"
     CDS             complement(784..>1259)
                     /locus_tag="PK264_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD75534.1"
                     /translation="LRLAEWREIDLKGAVWTIPAARMKMRREHRLPLSRQSLSILSNL
                     QAHTGGGKYVFPGYGMSGGYGRRIEPRPLSENTLNSALRRMGFAQDEMSAHGFRAVAS
                     TMLNESGLFSADAIERALAHQDPDAVRRAYARGDFWEERVRLMQWWADRVDALRS"
     assembly_gap    1260..1392
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1393..2003)
                     /locus_tag="PK264_06290"
     CDS             complement(<1393..2003)
                     /locus_tag="PK264_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HRD75535.1"
                     /translation="MALTDIAIRNLKSADSIKKLSDGEGLQLWVTPAGGKIWKLAYRI
                     EGLQKTLTIGPYPEISLADARAKRDEARKLLVSGGDPAQQKRQSKLTQTNNAAQTFSA
                     IAAEVVDKKRREQRSESTLTKLEWLHAFANGGFGKRPISEITAAEILAVLRREQDKGH
                     RETAKRLRAVIGEVFRYAIATGRAASDPTYALRGALLTPKVTHR"
     gene            complement(2179..2254)
                     /locus_tag="PK264_06295"
     tRNA            complement(2179..2254)
                     /locus_tag="PK264_06295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2219..2221),aa:Thr,seq:cgt)
     gene            complement(2344..>2796)
                     /locus_tag="PK264_06300"
     CDS             complement(2344..>2796)
                     /locus_tag="PK264_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRD75536.1"
                     /translation="GIVGGQVPLGRAISKADKDTRTPYNTYHIPGLPPTPICNPGRSA
                     LEATLNPARTSDLFFVVDLPTGRHTFTTNYKDHLAAVALLRKSERERAAAAGQPPPPA
                     DPQPASPAKAAAVVENAKLDPPFQPFIPDDPGPPVEPSAEKRGFRLFS"
     assembly_gap    2797..2810
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2811..3523)
                     /gene="mltG"
                     /locus_tag="PK264_06305"
     CDS             complement(<2811..3523)
                     /gene="mltG"
                     /locus_tag="PK264_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRD75537.1"
                     /translation="MKALNGLMTFAFMCFIAMAGIGYFLMNELDRPGPLAQSAVLVVP
                     KGAGPQEIASRLEKEGMIRDSMVFVAAYRAQQMLALGGGRPVQFRAGEFEIQKGASLR
                     QVLDSLMFGRAILHSITIPEGLTSHQIVERLRADPNLTGEIEVVPPEGSLLPDTHKFA
                     RGTTRQELLDRMLADRKRFLAQVWEKRQAELPFKTPEEALIMASIIEKETGRADERAR
                     VAAVFVNRMRKGMKLDSDPT"
     gene            complement(3817..5079)
                     /gene="fabF"
                     /locus_tag="PK264_06310"
     CDS             complement(3817..5079)
                     /gene="fabF"
                     /locus_tag="PK264_06310"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRD75538.1"
                     /translation="MRRVVVTGLGLVTPLGCGVEATWKRLLASESGARRVTEFDVSDI
                     SCQIANFIPRGDGSNGTFNADQWMDPKDQRRVDDFIVFAMAAATQALKDSGFEAKTKE
                     QQERAGVLIGSGIGGLNGIAETALLLKEKGPRKVSPFFIPGRIINLASGYVSIKHGLK
                     GPNHAVVTACSTGAHAIGDASRLIAYGDADVMVAGGAESPVCRIALAGFAACRALSTG
                     FNDRPTKGSRPYDKDRDGFVMGEGAGIMVLEALEHAKARGARIYAEVIGYGLSGDAYH
                     ITAPEETGDGAFRCMQMAVKRAGIQPSDIDYVNAHGTSTPMGDEIELGAVERLFGNSK
                     AKLSMSSTKSAIGHLLGAAGSVEAIFSVLAMRDNVAPPTLNLDNPSVETAIDLVPHSA
                     RKKQIDTVLSNSFGFGGTNASLILRRAA"
     gene            5386..>5646
                     /locus_tag="PK264_06315"
     CDS             5386..>5646
                     /locus_tag="PK264_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75539.1"
                     /translation="MASNKSAVRGAYGVLLATALAVGAGVSYAQAPAEKVLRIGMTAA
                     DIPKTNGQPDQGFEGNRFTGIPMFDALTHWDLSSADKPSVLVP"
     assembly_gap    5647..5661
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5662..7058
                     /locus_tag="PK264_06320"
     CDS             <5662..7058
                     /locus_tag="PK264_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75540.1"
                     /translation="WAADAKDQRKWVFKLRPGVKFHDGTAVNADAIVWNVDKVLNKDA
                     EHFAPDQIGVTVSRMPNLLSAKKVDELTVELTTKEPDSFLPINLTNLFIASPAHWKKK
                     LTAVPAAVADPKERAKQAWVAFAADASGSGPFRMAKFVPRERLELVRNPDYWDAKRRP
                     KIDRVVLLPIPEPSARTAALLSGQVDWIEAPAPDAIPQIKSKGFVVHTNAQPHVWPWQ
                     FSFAENSPWLDKRVRYAANLCVNRDELKQLLGGLMEVPKGTVPPGHPWWGNPKFEIKY
                     DLAAAKKLMTEAGFGPTKRLKVKTITSASGSGQMQPVAMNEFLQQALKDCFFDVELEV
                     VEWNALFTNWRAGAKDPTSRGAHATNVTFAAMDPFFAMVRFSSTKTFPPVSNNWGYFG
                     NEVSDKLIAAARNAFEDKARDAALANLHAYIVDEAPFLWVAHDVGPRAMSAKVKNVVQ
                     PKSWFIDLAPMTLD"
     gene            7142..8095
                     /locus_tag="PK264_06325"
     CDS             7142..8095
                     /locus_tag="PK264_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD75541.1"
                     /translation="MLVYLAKRIVFTLPIMLGVAIVCFLLVHLAPGDPLVSVLPPDAS
                     EALKQQMMALYGFDRPLVEQFGRWLGRALAGDLGTSIRTSRPVVVEVAKAVSNTLMLA
                     ALATIIGFILGTLLGFIAGTFRDTWIDKASSFLSVIGVSVPHYWLGMVLVIIFAAQLG
                     WLPPTGAGPGGSENWRWDWAHIRYMILPAVTMSVIPMGIIARTVRALVAEIMSQEFIA
                     GLRAKGLTRLGVFRHVVKNVAPTALAVMGIQLGYLLGGSILIETVFAWPGTGLLLNDA
                     IFQRDLPLLQGTILVLASFFVVLNLVVDVLQAALDPRISRS"
     gene            8100..9047
                     /locus_tag="PK264_06330"
     CDS             8100..9047
                     /locus_tag="PK264_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD75542.1"
                     /translation="MADAALTRAPEAIVRSPGYWSTVLRRFFRDPVAVGALIVILLIV
                     LMALLAPWIAPGDPYKGMALRRLKPPGTEGFPLGTDELGRDMLTRLIYGARLSLLMGV
                     TPVFLALFFGSLIGITAGYVGGWVNSILMRTMDVFFAFPSVLLAIALAGAMGDGGKFA
                     AVWATINMVLPVELSPVIFTGLLALTIVFVPPIARVAESVTTSMRGREFVEAARASGA
                     GSLSIIRHHILGNVLGPIFVYAMSLIAVCMILASGLSFLGLGVKPPEPEWGLMLNTLR
                     TAIYTQPLLCALPGIMIFITSISFNLMTDGLRSAMDIKA"
     gene            9055..9499
                     /locus_tag="PK264_06335"
                     /pseudo
     CDS             9055..9499
                     /locus_tag="PK264_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970025.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     assembly_gap    9500..9527
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9528..10129
                     /locus_tag="PK264_06340"
     CDS             <9528..10129
                     /locus_tag="PK264_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75543.1"
                     /translation="PARFAGRYPHELSGGQRQRVNIARALALEPRLLILDEAVSALDK
                     SVEAQVLNLLLDLKEELKLTYIFISHDLEVVRFISDRVMVMYLGRIAEIGPTEQLYRA
                     PRHPYTAALLGSMLSMDPDKRTQDAPLTGDPPNPISPPPGCRFHTRCKHAEEVCAGTE
                     PPLLAVGGEVGLVSCHMSHEGAGHSRSPKPSMHALRVRR"
     gene            10126..11112
                     /locus_tag="PK264_06345"
     CDS             10126..11112
                     /locus_tag="PK264_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75544.1"
                     /translation="MSEPALTIEGLNVTFTGGRTQVRAVDGVDLSLKAGEVLALLGES
                     GSGKSVTLRSILRLHDERRTRIEGRIRADGTDVMALSGGALSDFRGKTVSMIFQEPML
                     AMDPVYTVGRQIIEAIRRHEPVSDAEARRRALELFERVRIPSPERRLDAYPHEMSGGM
                     RQRAMIALALACRPKLLLADEPTTALDATVQVQILLLLRELQRELGLSVIFVTHDIGV
                     GVEVADRIAVMYAGRIVEIGPVRDVIRTPRHPYTIGLLSTRAHGGRLSGRRLPAIPGS
                     PPDLANLPPGCAFAPRCRLADQTCGVSQPGAVAIAADHLVRCYKTAETKRLP"
     gene            11131..11865
                     /locus_tag="PK264_06350"
     CDS             11131..11865
                     /locus_tag="PK264_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRD75545.1"
                     /translation="MLIRVVNPNTTIAMTATIAAAAREAASPTSRIDAVTSPTGPVSI
                     EGYYDEALCLPGLLLEIAKGEALAAAGHVIACFDDTGLDAARQVARAPVVGIGEAAYH
                     AATLVAHRFTIVTTLGRSVPALETNLLRYGFERRAKVRAAEVPVLALDDPRSDARGRI
                     NAEIARALREDRAEAIVLGCAGMAKLARDLSAEHGCPVIDGVGVAVKLVEMLATLGHR
                     TSKRVAYEWTGAKQLSGPIADLMGPR"
     gene            complement(11890..12336)
                     /locus_tag="PK264_06355"
     CDS             complement(11890..12336)
                     /locus_tag="PK264_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75546.1"
                     /translation="MVLRQLKRRAVAALAVSSALALTGCAAGDVELNGKIFDALGVSA
                     ATSKPSREPKLAARAPLVLPPSADRLPDPNAVPVAASGDPAWPVDADQKRVGTVADAA
                     RKHTEFCRDGNWKEKAMRDDIAAAKGPAGNCNDGSIFSAFKNLTTK"
     gene            12743..13429
                     /locus_tag="PK264_06360"
     CDS             12743..13429
                     /locus_tag="PK264_06360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75547.1"
                     /translation="MRRVECAGTSEGGDPRGVEVRSIGIDDYSAVRYVHGTAFKSIGL
                     SFLDEAELNPLLAPFESIEYTESLMSEDLVGAWIGSELVGTAVWLAADDTGRSARITS
                     VYVRPMFTRLGVGRMLVREDETRARRHGYRSFSARATPITAGFYQKLGYAVTSHGSIP
                     VDKERGLPLTFLRRHDAAVVEQGLEHSAPAARPGARTLPKGSRSVANVVDIMTAAASK
                     TPPLPKFADQ"
     gene            complement(13445..>14216)
                     /locus_tag="PK264_06365"
     CDS             complement(13445..>14216)
                     /locus_tag="PK264_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD75548.1"
                     /translation="TGSSGGVPRVGNDWGACSPENPRNRRRRCSALLERFEGGRATRV
                     LIDTSPDLREQALTHRIEHLDAVLYTHDHADHTHGIDDLRFISYRMKRRINVYLDQPT
                     RVSLEGRFGYCFSTPPGSAYPPIVRAHEIEAGTPITVTGAGGAVEALPVPQEHGDIPS
                     LGFRIGNLAYSPDISGLSDGAADMLLGLDVWIVDALRYIPHPSHWSVKQSLEWIERLA
                     PKRAILTHLHIDLDYDALKRELPAHVEPAYDGMVIDFR"
     assembly_gap    14217..14236
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14251..15057)
                     /locus_tag="PK264_06370"
     CDS             complement(14251..15057)
                     /locus_tag="PK264_06370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRD75549.1"
                     /translation="MLVDHHCHLDFPDLAADREGVLARARAAGVGAMITISTRVRRFA
                     EITAIAEAHSEVYCSVGTHPHYAHEEPDLTPEQLAGLARHPKCVAIGEAGLDYFYQKS
                     PREAQADGFRRHIAAARLSALPLVIHSRDADADTIAILEDEMSSGRFAAVLHCFTGGR
                     ELAFRAVELGLYVSFSGVITFSKSEALREIAAALPLDRLLVETDAPYLAPAPHRGKRN
                     EPAFVAHTAAALAKVRGMPVAALAMATTENALRLYAKIPRVALGLTGAAA"
     gene            15426..15662
                     /locus_tag="PK264_06375"
     CDS             15426..15662
                     /locus_tag="PK264_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75550.1"
                     /translation="MTLIRTLALAAAVTLPPAMIAAPAIAQQVNSGVSANIDPNKLKN
                     SVGLTREQQCRLGLINPRACAPGGVRPSAPKRPN"
     gene            15963..16532
                     /locus_tag="PK264_06380"
     CDS             15963..16532
                     /locus_tag="PK264_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75551.1"
                     /translation="MSRRIALAGALACALLSAAPAANAADLGDRYAPGSAHDPYDDPR
                     YADIYRHPPPPSSYPPPAPRYTPPSGYSYVPPPARHDDGYGRCVPSESIRVRLMNEGW
                     SDFRGIRLRDDIAVLEARRPSGQLFRLKIDRCSGQILNALQVEPGAAPYAYGAPGYGP
                     PGYATRPYAAAPYGRPGGWPGTRYYYRPF"
     gene            16751..17998
                     /locus_tag="PK264_06385"
     CDS             16751..17998
                     /locus_tag="PK264_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD75552.1"
                     /translation="MRKPFPMQGRSPADVLSDLARFKTGDADWRGGRVPLFVFKADQG
                     VRDLGADAFMAYFDENALGAKRAFPSVKRMEDEVVGMALDLFRAPEDGQGFMTTGGTE
                     SIVLAVQCCRDWSRAARGDPRHRGNIVCSEATHPAFDKAGRLMDLEVRRVPVGADYRA
                     DPAALEAALDADTIMLVASAPSFPFGMIDPVTEIAAVAERRGIWLHVDACVGGYLAPF
                     VRAIGRDIPEFDFAIPGVSSLSADLHKFGFTPKPASTVLYRRPSLAGHQGFDGDVWPS
                     GRFTTATIVGTRPAGGVAAAWATLNHLGRQGYERIAGELMAFVDRYKAGIRAIDGLEI
                     IGAPHLSLVAYGSTRVDIFRVAELMSERGWVPGLLQRPKAIHRMMSLLHEPSLEAYLA
                     DLRAAVTMVKAEQSGPSRLSAQY"
     gene            18252..>18597
                     /locus_tag="PK264_06390"
     CDS             18252..>18597
                     /locus_tag="PK264_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRD75553.1"
                     /translation="MLRKVRGTLIGVSVMSAVINMLALTGSFYMLQIYDRVLSSYSVA
                     TLIVISALAAWLFLIQGVLDILRAQILIRVGNIVDRELMPKALTASMRLPLYGASEGD
                     ARLPVRDVDTIRG"
     assembly_gap    18598..18607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18608..>19085
                     /locus_tag="PK264_06395"
     CDS             <18608..>19085
                     /locus_tag="PK264_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRD75554.1"
                     /translation="GPAAIADLPWFPIYLVFVFILHPWLGILATSGVAILVAITIATE
                     RITGRHIQEQTRLGLLRQGVLDSGVRNAEVARAMGFSGRVAERFNMSSRQLVALQTST
                     SDITGSLSGASRVLRAMLQSAVLGLGAYLTIKGQMSAGAIIAASIAASRALAPIELA"
     assembly_gap    19086..19135
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19136..19938
                     /locus_tag="PK264_06400"
     CDS             <19136..19938
                     /locus_tag="PK264_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD75555.1"
                     /translation="LLATLPIEPPTIELKPPVASLRLEGVTVAAPGTQKVIIANASLE
                     LRAGQALGVIGPSAAGKSTLARAAVGVWPLMRGSVRLDGAALDQWSAEQIGRHIGYLP
                     QSVELFEGTISENIARFETRPDEQAIIAAAEVAGCHGMIVKIQDGYGARIGAGGVMLS
                     AGQRQRIGLARALYRSPFLVVLDEPNSNLDQEGETALVAAIQKVRQRGGIVVLVAHRP
                     SVLAACDMAAFVTNGQIAAFGPRDEVLKKVLRPQQPKPPNGPHDREPR"
     gene            19935..>20238
                     /locus_tag="PK264_06405"
     CDS             19935..>20238
                     /locus_tag="PK264_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRD75556.1"
                     /translation="MSTPAVAAEGGLSARLWAAAAVVLLLLGGTGTWIAATSLAGAVV
                     TSGFVVVDGNVKKVQHPTGGVVGAIEVTEGQHVEAGDLLVRLDETVIRNILRRAEFL"
BASE COUNT         3316 a         6712 c         6311 g         3629 t
ORIGIN      
        1 catggctcca tcctacttgg gagttggagc ctccggcaaa ccgggggcgg ttcaatccgc
       61 ccaccttcca gcatcgacaa ttgccttctt tgcggcaacc aatttgcgga cattgtgcaa
      121 cttggaccct cccaagcgca catcaacact ttgctcaaac tacgtcaagg tgataattgc
      181 cagatttggt cgaaaaccta ccgattatcg tcgtttagca tctcgaaaca tcttctttat
      241 cgtcgccatg tcaatcgccg tatcatgaaa agtattaggg tcttcttcat atgaaaaaga
      301 aagctctatg gaaattgcta aacctatcgc gattgcaaca tcttcgaatg atcctctttt
      361 cagagcgcgc gcccgccagt cattcttgcg ctgtggattg catcccaatc gttgacgcct
      421 ccccggatca tccgccaatt cgtaaaatgc ctgttgaaat gttttccgtg ggcgtccgct
      481 cactttaggc cgattttgtg gttcgcctcg ctgtccctta ggaaggtgag aaagttgaga
      541 tagctgctcg aaaataagta tctgtcgaca caatctttct atttctcttg ttttcaatag
      601 agccatttct agaaatggcc atatcattgg atcttttgct agataatttt ttactttcaa
      661 ttgatcaatt gggctcatga aacttatttt tcgcaataaa taggtcgctc ctatcaatcc
      721 tcttcctgga aatcttatta ttgtcatcgt gattccaatg aaactttcag tctatgttga
      781 atcttaactg cgcagcgcat caacgcggtc agcccaccac tgcatcaggc gcacacgttc
      841 ctcccagaag tcacctcggg cgtaggcgcg acgcacggcg tcagggtctt ggtgagctag
      901 cgctcgttca atggcgtcag cagaaaacag gccggattcg ttgagcatgg tggatgcgac
      961 tgctcggaaa ccatgcgcgg acatttcgtc ctgggcaaat cccatccgac gcagggcgct
     1021 gttgagcgtg ttctcgctca atgggcgcgg ttcgatgcgc cgcccatagc cgcccgacat
     1081 gccatagccg ggaaagacat acttgccacc acccgtgtgt gcttgaagat tgctaaggat
     1141 gctaaggctc tggcgcgaga gaggaaggcg gtgttcacga cgcatcttca ttctggcagc
     1201 cgggatcgtc cagacagcac cctttaggtc aatctcccgc cactcggcta agcgcagttn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nncggtgtgt caccttaggt gtcaacagcg cgccgcgaag ggcgtatgtc
     1441 ggatcagacg ccgcccgccc ggttgcaatg gcatagcgaa aaacttcgcc gatcacggcg
     1501 cgaagacgct tggccgtctc gcggtgtccc ttgtcttgct cacgtcgcaa gacagcaagg
     1561 atttctgccg ctgtgatttc ggagattggg cgcttaccaa agccaccatt ggcgaaggca
     1621 tgaagccatt cgagcttggt aagcgtgctc tccgacctct gctcccggcg cttcttgtcg
     1681 acaacctctg cggctatcgc ggagaatgtc tgtgctgcat tgttggtctg cgtcagcttc
     1741 gactgccgtt tctgttgggc tggatcgccg cccgagacca acagcttgcg cgcctcgtct
     1801 cgcttcgccc gtgcatctgc aagggagatt tcaggatacg ggccgattgt cagcgtcttc
     1861 tgcaggccct cgattcgata tgcgagtttc cagatcttgc ctccggcagg cgtcacccag
     1921 agctgcaaac cttcgccgtc agacaatttt ttgatcgaat cggcagattt caagttgcga
     1981 atggcaatgt cggtaagcgc catttgttgg tctccagccc gttggctcgt tcaaaagacc
     2041 aacaaggaga ccaacattct gttgggctac tgtgatccca tctgcgacac accggcgcaa
     2101 atggagacac aaaatcgctg aaaaatcaac gaaattgaga cttctggaga cgctcagaga
     2161 tattcaggaa agaggaggtg gtgccgcaag agggaatcga acccccgacc ccatcattac
     2221 gaatgacgtg ctctaccagc tgagctattg cggcacagga agcccgccaa taacgggtct
     2281 tgccgcccgg tgcaagcggc aagggcaaat ttctggcagc gcggatccgc gatggggggt
     2341 ctgtcagcta aagagccgga atccgcgctt ttccgccgac ggctcaacgg gggggccggg
     2401 gtcgtccggg atgaagggct ggaacggcgg gtccagcttg gcgttctcga caactgctgc
     2461 tgccttggct gggcttgccg gttggggatc ggccggcggc ggtggctgtc ctgcagcggc
     2521 tgcccgctca cgctccgact tgcgcaacag cgcgactgcg gcgaggtgat ccttgtagtt
     2581 cgtcgtaaag gtgtgccggc ccgtcggcag gtcgaccacg aagaataggt cgctcgtgcg
     2641 cgccggattg agcgtcgcct cgagtgcgct cctccccgga ttgcagatgg gcgtcggcgg
     2701 caatcccggg atgtggtatg tgttgtaggg tgtccgcgtg tccttgtcgg ctttagagat
     2761 cgcccggccg agcgggacct gccccccgac gatgccnnnn nnnnnnnnnn gtcggatcgg
     2821 aatcgagctt catgcccttg cgcatccggt tgacgaacac ggccgcgacc cgcgcgcgct
     2881 cgtcggcccg gcccgtctcc ttctcgatga tggaggccat gatcaatgcc tcctcaggcg
     2941 tcttgaaggg cagctccgcc tgccgcttct cccagacctg ggcaaggaaa cgcttgcgat
     3001 ccgccagcat gcgatcgagg agctcctggc gcgtagtgcc gcgcgcgaac ttgtgtgtgt
     3061 ccgggagcag gctcccctcg ggcggcacga cctcgatctc gcccgtgaga ttgggatcgg
     3121 cgcgcagccg ctccacgatc tgatggctcg tcaagccctc ggggatggtg atcgagtgca
     3181 agatcgcgcg cccgaacatc agtgaatcga gcacctggcg caggctcgcg cccttctgga
     3241 tctcgaactc gcccgcccgg aactgcaccg gccgcccgcc gccgagcgcc agcatctgct
     3301 gcgcccgata agccgcgacg aacaccatgc tgtcgcggat catgccttct ttctcgagcc
     3361 ggcttgcgat ctcctgcggg cccgcccctt tcggcacgac gagtacggcc gactgggcta
     3421 gcggccccgg tcgatcgagc tcgttcatga gaaaatagcc gatgcccgcc atggcgatga
     3481 agcacatgaa ggcgaaggtc atcagtccgt tcaacgcctt caagaacccg ctcgtccgag
     3541 tcggctcgga tggcacgcgc acgctgcgcc cccgcggcgc cggcgcccgc tgcggcacga
     3601 ggagctcgtt cgggctgtgc ggcagaaggc ggggtgctcc cgtcccggtc cgcacagtgg
     3661 gctgtgcatc gctgcgctcg ttcattgcca cgttcgctcg ttgctgcgtt tgcccgctgc
     3721 ggtagacagc ttcagaaaat caccgccgac cctaacgcca attatggcgc gaaggtggac
     3781 gcagatgcga cagagtgaat gcgttccagc gtccgttcag gcggcccgac gaaggatcaa
     3841 cgaagcattg gtgccgccaa agccgaacga attcgagagg acggtgtcga tttgcttctt
     3901 gcgcgctgaa tgcggcacga gatcgatcgc cgtctccacg gatggattgt cgaggtttag
     3961 tgtcggcggc gccacgttgt cgcgcatggc gagcaccgag aaaatggctt cgaccgagcc
     4021 cgccgcgcca aggagatgcc cgatcgccga cttggtcgag gacatggaaa gcttggcctt
     4081 ggaattgccg aacagccgct cgaccgcacc gagttcgatc tcgtcgccca tcggcgtcga
     4141 tgtgccgtgc gcgttcacgt agtcgatgtc gctcggctgg atgccggcgc gcttcaccgc
     4201 catctgcatg cagcggaagg cgccgtcgcc cgtctcctcg ggcgcggtga tgtgataggc
     4261 gtcacccgaa agcccgtagc cgatcacctc ggcatagatc cgagcgccgc gcgccttggc
     4321 atgctcgagc gcctcgagca ccatgatgcc ggcgccttct cccatcacga aaccgtcgcg
     4381 atccttgtca taggggcgcg agcccttggt cggccgatca ttgaagccgg tcgagagcgc
     4441 ccggcaggcc gcaaagcccg ccagcgcgat gcgacagacg gggctttcag cgccgcccgc
     4501 gaccatgacg tcggcatcgc cgtaggcaat gagccgcgat gcatcgccga tggcgtgcgc
     4561 tcccgtcgag caggccgtga cgaccgcgtg attcgggccc ttgaggccat gcttgatcga
     4621 gacgtagccg ctggcgagat tgatgatccg tcctggaatg aagaacggtg acaccttgcg
     4681 cggccccttc tccttgagaa gcagcgccgt ctccgcgatg ccgttgagtc cgccgatgcc
     4741 ggagccaatg agcacgcccg cgcgctcctg ctgctccttg gtcttcgctt cgaagccgct
     4801 gtccttcaat gcctgggttg ccgccgccat ggcgaagacg atgaagtcgt cgacacggcg
     4861 ctggtccttc gggtccatcc attggtcggc attgaacgtg ccgttgctgc catcaccccg
     4921 gggaatgaag ttggcgatct ggcagctgat gtccgacaca tcgaactcgg tcacgcggcg
     4981 cgcgccgctt tcgctcgcca gcaggcgctt ccaggtggcc tcgaccccgc agccgagcgg
     5041 tgtcacaagg ccaagtccgg tgacaacgac acgtctcatt ggtcactatc ctcaccagga
     5101 ttccggtcac gtgcaaatgg ttcgggtcat cagaaaccac gtcgccgacg cgcctgcaag
     5161 cctcccctcg agttctccgc gcggcgagcc gacctcaccg attgcctcga tgcgcgccac
     5221 ttttagcgaa agttttgtgc agcctctatt tgcagcaata ccgccccttg ttcggccggg
     5281 gaggacgtgc ttcgatgcat cgggctcggg cagtcggtcg cgtggaccaa caagctgtct
     5341 gcccatgctg ctccatcagc ctccatcgcg aaggacagga cctcgatggc ttccaacaag
     5401 agcgccgttc gtggcgcgta tggcgttctg ctcgccacgg ctctggccgt cggtgccggc
     5461 gtcagctacg cgcaggcacc ggcagaaaag gtgctccgca tcggcatgac cgcggccgac
     5521 attccgaaga ccaatggcca gcccgaccag ggattcgagg ggaaccgctt caccggcatc
     5581 ccgatgttcg atgccctaac gcactgggat ctgtcgtcgg ccgacaagcc gagcgtgctt
     5641 gtccccnnnn nnnnnnnnnn nagtgggcgg ccgacgccaa ggaccagagg aagtgggtct
     5701 tcaagctccg ccccggcgtc aagttccatg acggtaccgc cgtcaatgcc gacgcgatcg
     5761 tgtggaacgt cgacaaggtc ctcaacaagg acgccgagca cttcgcgccg gaccagatcg
     5821 gcgtgaccgt ctctcgcatg ccgaacctgc tgtcggcgaa aaaggtcgac gagctgaccg
     5881 tcgagctcac cacgaaggag ccggacagct ttctgccgat caacctgacg aacctcttca
     5941 ttgcctcccc cgcgcactgg aagaagaagc tcaccgcggt gccggcggcc gtagccgacc
     6001 ccaaggagcg cgccaagcag gcctgggtcg ccttcgccgc ggacgcctcg ggctccggcc
     6061 ccttccggat ggcgaagttc gtgccgcgcg agcgtctcga gctcgtccgc aaccccgact
     6121 actgggatgc caagcgccgt ccgaagattg atcgcgtcgt gctgctgccg atccccgagc
     6181 cgagcgcgcg gacggcggct ctgctctcgg gccaggtcga ctggatcgaa gcgcctgctc
     6241 ccgatgcgat cccgcagatc aagtcgaagg gcttcgtcgt ccacaccaat gcgcaaccgc
     6301 acgtctggcc gtggcagttc tcgttcgccg agaactcacc ctggctcgac aagcgcgtgc
     6361 gctatgcggc caacctctgc gtcaatcgcg acgagttgaa gcagctgctc ggcggcctca
     6421 tggaggttcc gaagggcacc gttccgcccg gccatccctg gtggggcaat ccgaagttcg
     6481 agatcaagta tgatctcgct gccgccaaga agctcatgac ggaggcaggc ttcggcccga
     6541 cgaagcgact gaaggtcaag acgatcacgt cggcctcggg ctccggccag atgcagcctg
     6601 tcgccatgaa cgagttcctg cagcaggcgc tgaaggactg cttcttcgac gtcgagctcg
     6661 aggtcgtgga gtggaatgcc ctcttcacca actggcgcgc gggtgcgaag gacccgacgt
     6721 cgcggggcgc gcatgcgacc aacgtgacct ttgcagccat ggaccccttc ttcgccatgg
     6781 tccgcttctc ctcgaccaag acgttcccgc ccgtctcgaa caactggggc tacttcggca
     6841 acgaggtgtc cgacaagctg atcgcggcgg cccgcaatgc cttcgaggac aaggcgcgcg
     6901 acgccgcact cgccaacctg cacgcctaca tcgtcgacga ggcgcccttc ctgtgggttg
     6961 cccacgacgt tgggccgcgc gccatgtcgg cgaaggtcaa gaatgtcgtg cagccgaaga
     7021 gttggttcat cgaccttgcg ccgatgacac tcgactgacg ccgcgaccac ggacttgaac
     7081 cccgacgccc cggccctcaa ccggggcgtc attaggtcaa gctgcaggtc tggcggctcg
     7141 aatgctcgtc tatctcgcca agcgcatcgt gttcacgctg ccgatcatgc tcggcgtggc
     7201 gatcgtctgc tttctgctcg tccatctcgc ccccggagat ccgctcgtct ccgttctgcc
     7261 gcccgacgct tccgaggcat tgaagcagca gatgatggcg ctctacggct tcgaccggcc
     7321 tctcgtcgag cagttcggcc gctggctcgg ccgtgcgctt gcaggcgacc tcggcacctc
     7381 gatccgcacc agccggccgg tcgtcgtcga ggtcgccaag gccgtctcga atacgctgat
     7441 gcttgccgct ctcgcgacca tcatcggctt tatcctcggc acgctgctcg gcttcattgc
     7501 cggcaccttc cgcgacacct ggatcgacaa ggcctcctcg tttctgtccg tcatcggcgt
     7561 atcggtgccg cactattggc tcggcatggt gctggtgatc atcttcgcgg cccagctcgg
     7621 ctggctgcct ccgaccggcg ccggacccgg cggctccgaa aactggcgtt gggactgggc
     7681 gcacatccgc tacatgatcc tgcccgccgt gacgatgtcg gtgattccga tgggcatcat
     7741 cgcgcgaacg gtgcgtgcgc tcgtcgcgga gatcatgtcg caggagttca tcgcgggact
     7801 gcgcgccaag gggctgaccc gcctcggcgt cttccgccac gtcgtgaaaa acgtcgcccc
     7861 gaccgcgctg gccgtcatgg gcattcaact cggctacctg ctcggcggct cgatcctgat
     7921 cgagacggtg ttcgcctggc ccggcaccgg cctcctcctg aacgacgcga tcttccagcg
     7981 cgacctgccc ctgctccagg gcaccatact ggtgctcgcc tccttcttcg tcgtcctcaa
     8041 tctcgtcgtc gatgtgctgc aggcagccct cgacccgcgc atatcgagga gctgaggcga
     8101 tggccgacgc agctctcacc cgcgctcccg aggccattgt ccgctcgccc ggctactggt
     8161 cgacagtgct ccgccgcttc ttccgcgatc cggtcgcagt cggggcgctg atcgtcattc
     8221 tgctcatcgt gctgatggcg ctgcttgccc cctggatcgc gccgggcgac ccctacaagg
     8281 gaatggcgct ccgccgcctg aagccgcccg gcacggaagg cttcccgctc ggcacggacg
     8341 agctcggccg cgatatgctg acccgcctca tctacggggc acggctgtcg ctcctgatgg
     8401 gcgtgacgcc cgtattcctg gcgctcttct tcggctcgct gatcggcatc acagccggct
     8461 atgtcggcgg ctgggtcaac tcgatcctga tgcgtaccat ggacgtgttc ttcgcgtttc
     8521 cctcggtgct gctggcgatc gcgcttgccg gcgccatggg cgatggcggc aagtttgcgg
     8581 ccgtctgggc gacgatcaac atggtgctac ccgtggagct gagcccggtg atcttcaccg
     8641 ggcttcttgc gctcaccatc gtcttcgtgc cgccgatcgc gcgcgtcgcg gagagtgtca
     8701 cgacgagcat gcgcggccgc gagttcgtcg aggcggcgcg cgcgagcggc gccggctccc
     8761 tctcgatcat ccgccaccac atcctcggca acgtgctcgg cccgatcttc gtctacgcca
     8821 tgagcctcat cgcagtgtgc atgatcctcg cctcgggcct ctcgttcctc ggcctcggcg
     8881 tgaagccgcc cgagcccgag tgggggctga tgctcaacac gcttaggacg gccatctata
     8941 cgcagccgct gctctgcgcg ctccccggca tcatgatttt catcacctcg atctcgttca
     9001 acctcatgac cgacggactc cgctcggcca tggacatcaa ggcgtgaggg cgcaatgctg
     9061 accgccggcg agatcgcgga ctggggtgcc gatggcacgg ggcgaggcgc gccgctcctc
     9121 gaggtcgaag gcctcacccg gcatttccct ctgaaggggg gcctgctcgg gcgcgcgcgg
     9181 ggcgtcgtgc gcgccgtcga cgacatcggc ttcgtggtgc atgccggaga gacgctcggc
     9241 atcgtcgggg aatccggctg cggcaagtcg acgaccgcgc ggctccttat gagcctcatc
     9301 tctcccgacc aggggcgcat ccgctttgcg ggaaaggacg tcggcggcgg cggtctcgcg
     9361 ctcaaggcct accgcgcgca agtgcagatg gtgttccagg acagctacgc ctcgctgaac
     9421 ccgcgcctca cggtcgagga cagcatcgcc tacggcccaa gcgtgcacgg cgtctccgca
     9481 caagatgcga tcggccgggn nnnnnnnnnn nnnnnnnnnn nnnnnnncgc ccgcgcgctt
     9541 cgccggccgc tatccgcacg agctttcggg cggccagcgc cagcgtgtca acatcgcccg
     9601 cgcactcgcg ctcgagccgc gcctcctcat cctcgatgaa gcggtctcgg cactcgacaa
     9661 gtcggtcgag gcccaggtgc tcaatctgct cctcgacctc aaggaggagc tgaagctcac
     9721 gtacatcttc atcagccacg acctcgaggt cgtgcgcttc atctccgacc gggtgatggt
     9781 gatgtacctc gggcgcatcg ccgagatcgg cccgacggag cagctctatc gtgcgccgcg
     9841 ccacccctat acggcggccc tcctcggctc catgctgtcg atggaccccg acaagcgcac
     9901 gcaggacgcg ccgctcaccg gcgatccgcc gaacccgatc tcgccgccgc ccggctgccg
     9961 ctttcacacc cgctgcaagc acgccgagga ggtctgcgcc gggaccgagc cgcctctcct
    10021 cgctgtcggt ggcgaggtcg gcctcgtctc ctgccacatg tcgcacgagg gcgccggaca
    10081 ctcgcgctcg ccgaagccgt cgatgcacgc gctacgggtg cgccgatgag cgagccggcg
    10141 ctcacgatcg aggggctcaa cgtcaccttc accggaggcc gtacgcaggt gcgcgcggtc
    10201 gacggcgtcg acctctcatt gaaggccggc gaggtgctgg ccctgctcgg cgagtcgggc
    10261 tccggcaaga gcgtgactct ccgctcgatc ctgcgcctcc atgacgagcg ccgcacgcgc
    10321 atcgaaggcc ggatccgcgc cgacggcacc gacgtcatgg cgctctcggg cggcgccctc
    10381 agcgatttcc gcggcaagac cgtatcgatg atcttccagg agccgatgct ggcaatggac
    10441 ccggtctata ccgtcggccg ccagattatc gaggcgatcc gccggcacga gccggtctcc
    10501 gatgccgagg cgcgccgtcg cgccctcgag ctcttcgagc gcgtccgcat cccgagcccc
    10561 gagcgacggc tcgatgccta cccgcacgag atgtccggcg gcatgcgcca gcgcgccatg
    10621 atcgcgctcg cccttgcctg ccggccgaag ctgctgctcg ccgacgagcc gacgaccgcg
    10681 ctcgatgcga cggtgcaggt gcagatcctc ctcctgctcc gcgagctcca gcgcgagctc
    10741 ggcctctccg tcatcttcgt cacgcacgat atcggcgtcg gcgtcgaggt cgccgaccgg
    10801 atcgctgtca tgtacgccgg ccgcatcgtc gagatcgggc cggtgcgtga cgtgatccgc
    10861 acgccccgcc acccctacac gataggcctt ctctcgacac gcgcgcatgg cggtcgcctc
    10921 agcggccgac gcctcccggc gatcccgggg agcccgcccg acctcgccaa cctgccgccg
    10981 ggctgcgcat tcgcgccgcg ctgcaggctg gccgatcaga cctgtggcgt gtcgcagccc
    11041 ggtgcggtcg cgatcgccgc cgatcacctc gttcgatgct acaagacggc cgagaccaag
    11101 cggctacctt gagccggcag gaggcaatcg atgctgatcc gcgtcgtcaa tcccaacacc
    11161 acgatcgcca tgacggcgac gattgcagcg gcggcgcgcg aggcggccag ccccacgagc
    11221 cgcatcgatg ccgtcacatc accgaccggc ccggtgtcga tcgaaggcta ctacgacgag
    11281 gcgctctgcc tgccgggcct gctgctcgag atcgcgaaag gtgaagcact tgcggcagcc
    11341 ggccatgtca ttgcctgctt cgacgacacg ggcctcgatg ccgcgcggca ggtcgcaaga
    11401 gctccggtcg tcggcatcgg cgaggcggcc taccatgcgg cgacgctggt cgcgcatcgc
    11461 ttcaccatcg tcaccacgct cggccgtagc gtgcccgcgc tcgagaccaa tctgctgcgc
    11521 tatggcttcg agcgccgcgc caaggtccgc gcggcagagg tccccgtgct cgccctcgat
    11581 gacccgcgct ccgacgcgcg cggccgcatc aacgcagaga tcgcccgcgc actcagggag
    11641 gaccgcgccg aggcgatcgt gctcggctgc gccggaatgg caaaactcgc acgcgacctt
    11701 tcggccgagc acggctgccc agtcatcgat ggcgtcggag ttgccgtgaa gctcgtggag
    11761 atgctcgcca cgctcggcca ccgcacgtcg aagcgggtcg cctatgagtg gaccggcgcc
    11821 aagcagctga gtggcccgat cgccgacctc atggggccgc gctgatgcgg cctcgacctg
    11881 ctcgcgacct cacttcgtcg tcaagttctt gaacgccgag aagatcgagc cgtcgttgca
    11941 gttgccggcg ggtcccttgg cggcagcgat gtcgtcgcgc atcgcctttt ccttccagtt
    12001 tccgtcacgg cagaactcgg tgtgcttgcg ggccgcgtcc gccacagttc cgacgcgctt
    12061 ctgatccgca tcgacgggcc aggccggatc accggaggcc gcaaccggca ctgcgttcgg
    12121 atcaggcagc cggtccgcgc tcggcggcag caccaagggc gcgcgtgcag cgagcttcgg
    12181 ctcgcgagat ggcttggaag tcgcggcact gacaccgagc gcatcgaaga tcttgccgtt
    12241 gagctcgacg tcgcccgccg cgcaacctgt cagcgcgagc gcactcgaca ctgcgagggc
    12301 ggcgactgcg cggcgcttca gctgtctcag gaccatatcg gcatcctatg cgatcatgat
    12361 gcgatgagag cacgatggtg acggtgctta cgcctgcacc gccacgcctc gcccattcga
    12421 tctgtctaca gccagccaga gcaggagatt gcggcagctt ttcgaatctt gcgccgagat
    12481 taggcgggtg cggcacggga tgcaatcgtc gtggccaatt cgccacaggc acaggagcaa
    12541 tcgctcgggc cacagcggcc cgccagctgc gacggaggct cgagttaggt cgctgccgtc
    12601 aagtgcgatt tcgagggata atgttcctcc tcctgaattc gccgctttaa tgaactgaca
    12661 tggctatggt atacgtccgt ccacagcctg atgtagacgc gttttcggcg ggttgccggg
    12721 tcgacgggca ggggacgaag tcatgcggcg agttgaatgc gcaggcacca gcgagggggg
    12781 tgacccccgc ggcgtcgagg tcagaagcat cggcatcgac gattactctg ccgtccgcta
    12841 cgtccatgga acggcgttca agtcgatcgg gctctccttt ctagacgaag ccgagctgaa
    12901 cccgcttctc gcccccttcg aaagtatcga gtatactgaa agcctcatga gcgaggatct
    12961 cgtcggcgcc tggatcggca gcgagcttgt cggcaccgcg gtctggctcg ccgccgacga
    13021 taccggtcgc tcggcgcgca tcacgtcggt atatgtccgg ccgatgttca cccggctggg
    13081 tgtgggccgc atgcttgtcc gcgaggacga aacgcgcgcg cgtcggcacg gctaccgctc
    13141 gttcagcgcg cgggcgacgc ccatcacggc agggttctac cagaagctcg gctacgccgt
    13201 gacctcgcac gggtcgatcc ccgtcgacaa ggagcggggg ctgccgctga cgtttctccg
    13261 ccgccatgat gcggccgtgg tggagcaggg attggaacat tcggcccccg cggcacgtcc
    13321 cggcgcgcgc acattgccga agggatcgcg atcggtcgcc aacgtcgtcg acatcatgac
    13381 ggcggccgcc tcaaagacgc cgccgctgcc gaagttcgcc gaccagtagg ccgcgcggcc
    13441 tgccttacct gaaatcgatc accatgccgt cgtaagccgg ctcgacgtga gcgggcagct
    13501 cgcgcttcag cgcgtcgtag tcgagatcga tgtggaggtg ggtgaggatt gctcgcttcg
    13561 gcgcgagccg ctcgatccac tcgagcgact gtttcacgct ccaatggcta gggtgcggga
    13621 tgtagcgcag cgcatcgacg atccacacgt cgagcccgag cagcatatcc gccgcgccgt
    13681 ctgacaagcc cgagatgtcg ggtgaatagg caagattacc gatccggaag ccgagcgagg
    13741 gaatgtcgcc atgctcctgc ggcaccggca gcgcctcgac ggccccgcct gctcccgtga
    13801 ccgtgatcgg cgtgcccgct tcaatctcat gcgccctgac gatcggcgga taggcggagc
    13861 cgggcggcgt cgagaaacag tagccgaagc gcccctcgag cgacacgcgc gtcggctgat
    13921 cgagatagac gttgatgcgc cgcttcatgc ggtaggagat gaagcgcaga tcatcgatgc
    13981 cgtgcgtgtg gtcggcgtgg tcgtgcgtgt agagcaccgc gtcgaggtgc tcgatgcggt
    14041 gcgtgagcgc ctgctcgcgg agatccggcg atgtatcgat caggacgcgc gtcgcccggc
    14101 cgccctcgaa gcgctcgagc agcgctgaac acctgcgtcg ccgattgcgt gggttctccg
    14161 ggctgcaggc accccagtcg ttgccgacgc gcggcacgcc gccggacgag ccggtcnnnn
    14221 nnnnnnnnnn nnnnnnatgc tcagcgcggc tcatgccgcg gctcccgtca atccgagcgc
    14281 cacgcgcgga attttcgcat agaggcggag cgcgttctcc gtcgtcgcca tggcgagagc
    14341 cgccacgggc atgccgcgca ctttcgcgag cgctgccgcc gtgtgcgcga cgaacgccgg
    14401 ctcgttgcgc ttgccgcgat gaggtgccgg cgccagatac ggcgcgtccg tctcgacgag
    14461 caaccgatcg agcggcaaag ccgccgcgat ctcacgcagc gcttcggact tgctgaaggt
    14521 gatcacgccc gagaacgaga catagagccc gagctcgact gccctgaacg ccagctcgcg
    14581 cccgcccgtg aagcagtgca gcaccgccgc aaaccggcca ctcgacatct cgtcttcgag
    14641 aattgcgatc gtatcggcgt cggcgtcgcg cgagtggatg acgagtggca acgccgagag
    14701 ccgcgccgcc gcgatatggc gccggaagcc gtctgcttgc gcctcgcgcg gcgatttctg
    14761 gtagaaatag tctagccccg cctcgccgat cgcgacgcac ttcgggtgcc gcgccagccc
    14821 ggcgagctgc tcgggcgtca aatcgggctc ctcgtgcgca tagtgcggat gcgtaccgac
    14881 ggagcagtag acctcgctgt gcgcctccgc gatcgccgtg atctcggcga agcgcctgac
    14941 ccgcgtcgag atcgtgatca tcgcgccgac gcccgcggcg cgtgcacgcg caagcacccc
    15001 ttcgcgatcg gccgcgaggt caggaaaatc aaggtggcag tgatgatcga ccagcatcat
    15061 gtcattccaa gcctccgacg tgctgcgaca cctacatcaa cccacggcgc tgtgccaacc
    15121 ctgccagtga cgggctgatc tcggaacgaa agcttgtcgc ccacccatgc taccagcccc
    15181 cacgtagctc tctgtccgat gatgcgctga gcgcggcagc cgcggacacc tcgtgcggta
    15241 cgcccatgac gtcgcaggct tccttctgaa ggagcgggga tagggaacag gcgttggcat
    15301 ctttcaaagc tctgtgctgg cggtcacagc gagatgcggc tggcgcggtt atcttccgat
    15361 catcaggacg cgagcacatg agacgcgctc ctgacggaaa gtcactcaac cgactggagg
    15421 acgccatgac cctgatccgc acccttgctc ttgccgccgc cgtcaccttg ccgcctgcca
    15481 tgatcgccgc tcccgcgatt gcccagcagg tcaactcggg cgtctcggcc aacatcgacc
    15541 cgaacaagtt gaagaactcg gttggcctca cccgcgagca gcagtgccgc ctcggcctga
    15601 tcaacccccg cgcctgcgcg ccaggcggcg tccgcccgtc cgccccgaag cgtcccaact
    15661 gatcgctcgt gtcccgatcg cgaggttcgt tgccaccagc cgggagttcg gtgcgaacct
    15721 tgggcgtgcc gcggggcaaa tatgagcttc cgcagcgaca cactgccccc tcgtcatgcc
    15781 ggtgggccgc catctcggcg gctcaccgcg ccgaggtgac gatcatccga gccgcggcgg
    15841 cgcgactaac tcaatcctga acgcccaatg aatggcgcct gcagtggccg ttcaggttgc
    15901 tgcggccatt ttggggcgag ggaggcgcaa gctctcgggc cgtcgccagg aggaactcga
    15961 ccatgtctcg tcgcatcgca ttggcgggag cgctcgcatg cgcgctgctg tccgccgcgc
    16021 cagccgccaa cgccgccgac ctcggagatc gctacgctcc ggggagcgcc cacgatccct
    16081 atgacgatcc acgctatgcc gacatctacc ggcatccgcc gccgccgtcg tcctatccgc
    16141 cgccggcgcc gcgctacact ccgccatcgg gctacagcta cgtcccgccg cccgcgcgtc
    16201 atgacgacgg ctacggccgc tgtgtgccga gtgagtcgat ccgcgtccgc ctcatgaacg
    16261 agggctggag cgacttccgc ggcatcaggc tccgcgacga catcgccgtg ctcgaggcgc
    16321 gccgtccgag cggccagctc ttccggctga agatcgaccg ctgctccggc cagatcttga
    16381 atgcgcttca ggtcgagccg ggtgcggcgc cttatgccta tggcgcaccg ggctacggcc
    16441 cgcccggcta tgcgacccgc ccctatgcgg cggcacccta tggccgcccg ggcggctggc
    16501 ccggaacgcg gtactactac cgcccgttct gagagagatt tgctgaagcc ccgcgccccg
    16561 tcgagtcact cggcggggcg ccgtgttttc gaggcacgct gacgcccgcg gcgggatgct
    16621 ccacgtgtgt gttcagggcg cctcagaaac acgacacttc acgagttaca aacaggcgac
    16681 acatcacgag ctacggacag ctacggacaa ccggcttgcg ccatacgccg ctccgcatca
    16741 gactgccgga atgcgcaaac catttccgat gcagggccgg tcaccggccg atgttctgag
    16801 cgacctcgcc cggttcaaga ccggcgatgc cgactggcgt ggggggcgcg tgcccctctt
    16861 cgtgttcaag gccgaccaag gcgtcagaga cctcggggct gatgccttca tggcctactt
    16921 cgacgagaac gcgctcgggg ccaagcgtgc attcccgagc gtgaagcgca tggaggacga
    16981 ggtcgtcggc atggcgctcg atcttttccg ggcgcccgag gacggccagg gcttcatgac
    17041 gaccgggggc acggaaagca tcgtcctcgc ggtacagtgc tgccgcgact ggtcgcgggc
    17101 ggcgcgtggc gatccccggc atcgcggaaa catcgtctgc tcggaggcaa cgcatcccgc
    17161 tttcgacaag gcaggccgac tcatggacct cgaggtgcgc cgcgtgccgg tcggcgctga
    17221 ttatcgtgcc gatccggcgg cgctcgaggc ggcgctcgat gccgatacga tcatgctggt
    17281 cgcctccgcg ccgagcttcc cgttcgggat gatcgacccc gtcacggaga tcgcggcagt
    17341 ggcggagcgg cgcggtatct ggctgcacgt cgatgcctgc gtcggcggct atcttgcgcc
    17401 tttcgtcagg gccatcgggc gggacattcc ggagttcgat ttcgcgattc ccggcgtatc
    17461 ctcgctctca gccgacctcc acaagttcgg cttcacgccg aagccggcct cgaccgtgct
    17521 gtatcgcaga ccgtcgcttg ccgggcacca gggcttcgat ggcgatgtct ggccgagcgg
    17581 gcgcttcacg actgcgacga tcgtcggtac caggccagct ggaggcgtcg cggccgcgtg
    17641 ggcgacgctc aatcatctcg gccggcaggg ctatgagcgc atcgcgggcg agctgatggc
    17701 gttcgtcgat cgctacaagg cgggcatccg cgcgatcgac ggcctcgaga tcatcggcgc
    17761 gccgcatctc tcgctcgtcg cctacggctc gacgcgcgtc gacatttttc gcgtcgccga
    17821 attgatgagc gagcgcggct gggtgccggg cctcctgcag cgcccgaagg ctatccatcg
    17881 catgatgtcg ctgctgcatg agccctcgct cgaggcctat ctcgccgatc tgagggccgc
    17941 tgtcacgatg gtgaaggcag agcagagcgg accgagcagg ctttccgccc agtattgatc
    18001 gtgccgcatg accgcggaag agcgccggcc gacccgtccg cgcggattga cgatgattgc
    18061 ggtattgcgc gaaacattaa tgggttatca gccataagct aaacattcgc gtaaagatcg
    18121 tctgcgaata ctattccgtg ggatgcgttc tccaggtggg tggagcagcg cgccgatgag
    18181 agttgcgaag tgatcggggc caagcaatga gcgtgcaggg gaaacccgct cagcaaccac
    18241 tggttggtgg agtgctccgg aaagtccgcg ggacgctcat cggcgtttcg gtgatgtcgg
    18301 ccgtcatcaa catgctggca ctgaccggct cgttctacat gctccagatc tacgatcgcg
    18361 tgctctcgag ctattcggtt gcaacgctga tcgtgatctc ggcgctcgcg gcctggctgt
    18421 tcctgatcca gggtgtcctc gacattctgc gcgcacagat cctgatccgc gtcggcaata
    18481 ttgtcgaccg agagctgatg ccgaaggcgc tgacggcatc tatgcgcctg ccgctctacg
    18541 gtgcgagcga gggcgatgcc cgcctgccgg tgcgcgacgt ggataccatt cgcgggtnnn
    18601 nnnnnnnagg gccggcggcc atagcggacc tcccctggtt cccgatctac ctggtcttcg
    18661 tcttcatcct gcatccctgg ctcggtatcc tggcaacgag cggcgttgcc atcctggttg
    18721 ccattacgat cgccacggag cgcatcacgg ggcgccacat ccaggagcag acgcgcctcg
    18781 gactgctgcg ccagggtgtg ctcgacagtg gcgtgcgcaa cgccgaggtc gcgcgggcca
    18841 tggggttctc gggccgcgtt gccgagcggt tcaacatgtc gagccggcag ctcgtcgcgc
    18901 tgcagacgag caccagcgac atcaccggat cactctcggg tgcctcgcgg gtgctgcgcg
    18961 ccatgctgca gtcggccgtg ctcgggctcg gcgcctacct caccatcaag ggccagatgt
    19021 cggccggggc catcatcgcc gcctcgatcg cggcctcgcg ggcgctggcg ccgatcgagc
    19081 tcgcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctc
    19141 ctcgcgaccc tgccgatcga gccaccgacg atcgagctga agccgcctgt cgcctcactg
    19201 cggctcgaag gcgtcaccgt cgcggcacct ggaacgcaga aggtcatcat cgcgaatgcg
    19261 agcctcgagt tgagggcggg ccaggcgctg ggcgtgatcg gtccgagtgc ggcgggaaag
    19321 tcgacgctcg cccgcgcggc cgtcggcgtg tggccgctga tgcgcggctc ggtccggctc
    19381 gacggtgcgg cgctcgacca atggagtgcc gagcagatcg gccgccatat cggatacctg
    19441 ccgcagtcgg tcgaactctt cgagggcacg atctcggaga acatcgcgcg tttcgaaaca
    19501 aggcccgacg agcaggcgat cattgctgcc gcggaggtcg cgggctgcca cggcatgatc
    19561 gtgaagatcc aggacggcta cggcgctcgg atcggcgccg gtggcgtgat gctctcggcc
    19621 ggtcagcgcc agcgcatcgg gctcgcacgc gcgctctatc gctcgccgtt ccttgtggta
    19681 ctcgatgagc cgaactcgaa ccttgaccag gagggcgaga cggcgttggt cgcggcaatc
    19741 cagaaggtgc gccagcgcgg tggcatcgtc gtcttggtcg cgcaccggcc ttcggtgctc
    19801 gcggcttgcg acatggcggc cttcgttacg aacggccaga tcgccgcctt cggcccccgc
    19861 gacgaggtgt tgaagaaggt gctgcggcca caacagccga agccgccgaa tggtcctcac
    19921 gatcgagagc cgcgatgagt acaccagccg ttgccgccga gggcggcctt tccgcacgcc
    19981 tctgggccgc cgccgccgtc gtcctcctcc tgctcggcgg cacagggact tggatcgcgg
    20041 cgacgtcgct cgccggcgcg gtcgttacgt cgggcttcgt cgtcgttgac ggcaacgtga
    20101 agaaggtgca gcacccgacg ggcggcgtcg tgggcgccat cgaggtcacg gaaggccagc
    20161 atgtcgaagc gggcgatctg ctcgtccgcc tcgacgagac ggtcatcagg aacatcctgc
    20221 gacgcgccga gttcctgc
//
LOCUS       DAORNH010000041        19990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2096_length_19990_cov_2.650614, whole genome shotgun sequence.
ACCESSION   DAORNH010000041 DAORNH010000000
VERSION     DAORNH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19990
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_19990_cov_2.650614"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PK264_06410"
     CDS             complement(<1..983)
                     /locus_tag="PK264_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HRD75557.1"
                     /translation="MAGGLTFGERMRLAIIEADRQRRAALASMKRAPLIGWGQRSARA
                     DQMLILPPDVRIADPSFVRELEDGQLGLAEAVADISRASAFEVTPPSAAWLRELHGFA
                     WLRHLRAAQSDETRLIARGMVREWIGRYAAHRGVPWETSVVARRVLSWMTHAGFLLDD
                     TSADDYTFLTDSLGRQIVHLAASLHDSPPGYPRLLAIAALVTADLCIAGHDERLERDV
                     RTLRAELKRQVLADGGHETRNPGVLVEIMLDLVPLRQCFLARQQPVPDELAEAISGAR
                     GMLGHLRLGDGTIARFNGMGATSPEALATIFSHEAHAGLEAVDRNGPSGYVR"
     gene            complement(1118..3567)
                     /locus_tag="PK264_06415"
                     /pseudo
     CDS             complement(1118..3567)
                     /locus_tag="PK264_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
     gene            3699..4181
                     /locus_tag="PK264_06420"
     CDS             3699..4181
                     /locus_tag="PK264_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRD75558.1"
                     /translation="MKKSTNVTLDDIDRRLLKVVQEEGRISATELAERVGLSVSPCLR
                     RLRLLEEAGVVRGYAAQVDQAKVGLPVSVFVSIKLERQQKDAMSRFEAAVRRCPEVLE
                     CYLMTGPRDYLLRVVARDLADYERFVKETLTRFDGIANIESSFALAQVKQSGSLPIEG
                     "
     gene            4314..5732
                     /locus_tag="PK264_06425"
     CDS             4314..5732
                     /locus_tag="PK264_06425"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRD75559.1"
                     /translation="MPIQSIADSRAPLWLAGMLAVAATALAADRVLMSDLGGYSHMAL
                     AHSVLYLVTAGLLLSSRARAPSLFAILAIALVMRGIAMGVPHNVLTTDALRYVWDGRI
                     QWAGFNPYLYAPADERLAFLRDLVIFPNINQKETAVTIYPPFAEMIFMLAVKIADGIE
                     GMKLTMLAFEGLLVWSLVGCLDRLGISRDRVLIYAWHPLPVWEFASQAHIDAATAALI
                     TAALYARLVQRYWLTGMLLGLAAATKYYPALLLAVLLPRPPWLDRLLRRIPGVRSLIP
                     DEAAGARDECRTRQSLPDWLQLSLGFVATLIAVYLPYAWTAGPRVIGFLNRHLDNEGY
                     LAGYGFHLVWILRDFGLADPPARAYIAAAAVTLGALLAWAFLSRRARDVPIPSLVVIA
                     CAFVWLTSPHYAWYFAWIIPFAALAPSPAALLFTALAFVMYLPTIAPAHGQSIAYALV
                     FGVPLVVASVERWRARTRLPET"
     gene            5729..6970
                     /locus_tag="PK264_06430"
     CDS             5729..6970
                     /locus_tag="PK264_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75560.1"
                     /translation="MTLVPLPFRSRAFRSQWLGDLLTSWSFEIERLILGWYILVETKS
                     VVMLTLFGAMQYGGTLVAPLVGVLGDRIGPRRVLATMRLCYAVCAGTLLAVAWSGRLT
                     PTIVLFVGAIAGLVIPSDIGMRGALIAETQPREQLMAAMGAGRTTSDTARIGGALAGA
                     GLISAFGIVPTYFVVTCTYVASALLTLRAVASLPLEAGPRPERAPTSPWSDLKEGLAY
                     VWTTPHVLGGMAIACLVNLTAFPIMGGVLPYVAKEVYGLGQTGLGYLSAAIGVGAFIG
                     SIAVGMAGMRIAAGRVMLIATIAWYLLLLALALVTDPRAGALLLMLVGLAQSFSMVPL
                     AVMLLRTSEPRFRGRVMSVRMLAIYSLPFGLLGAGALIERFGFKATGSAYALLGLATT
                     LAIAIAWRRALWTDQAPGNAR"
     gene            complement(6967..9807)
                     /locus_tag="PK264_06435"
     CDS             complement(6967..9807)
                     /locus_tag="PK264_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD75561.1"
                     /translation="MRVKILGGGVAGLAVATALIRRAGITDVEVLERDSAERQATRKG
                     HGLILMQNGAKALRALDAGRVLDSCTAIRRALIQDAGGRSLDVGALDGVYCVTRSAII
                     EALSARLPAGVVKLGHAVDRIEAEGERVSRIVLADRPSIDLGEGDIVIGAEGWRSPLC
                     RALNPGFERPLSPVREIVTSTYLPELARQLGTTFIKTMFADRGVAFGLLAPTAERVIG
                     FMQFDARRHAPPTQDADAAALRSFLGRLLAGAPEVVRQYLERADYATAHVWHPPNSDL
                     PAELACGNGVLVGDAAHPLLPFTSQGIGAALEDAIILADRLAASETATGSVPEVLARF
                     VTDRRAAVEGFVLGGRAMLASFIGTAIPGGVPLIDGTATPIEDLVELSEADLRAAFLA
                     ADVDGDGRLGRQDFGRALALFEADISAGEEEGLFSEIDTDTSGSLSVEEILAALAGDG
                     PASPALARLRAGRSPRRQALYQRRSRIVDLFRTLAVGPAGGLDRSEFALALAMSGRVV
                     SEAEIKVQFDTLDRDGDGRLSLAELSQGVGSAPVAAEPGDDSNTLFSDDRVDRAMLRA
                     RAHNYRWATVEPDVIPLTAADPDFPAAREIVEAIRDYAASGYFSYGPGEGLGAFRETA
                     ARHFKGSRGIPCTAGEVLATNSAAAALYLVVRALLSPGEEALIADPCDFLFERSVVAA
                     GGTVRRFRLDEAAGWTVDVAAVERLVTPRTRILMICNPHNPTGRVWRREELEALTSVA
                     LRHKLWIVSDEVWSDIVFAPARLTSVAALSGEVARRTFTVYGFSKGYGMAGLRLGLVI
                     SPDTGQHRRLVSTTHFDETADGVSTLSQIAGIAALEHGAAWRAAFVEHLRSQRDVAVA
                     RLNRMPGVACALPEGTFVAFPDVSRLALGQDDVVERLAREFRIALVPGSPRFFGPGAA
                     GHVRLSLATSRAILDEALDRIERGVAAIGVKR"
     gene            9978..>11225
                     /locus_tag="PK264_06440"
     CDS             9978..>11225
                     /locus_tag="PK264_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HRD75562.1"
                     /translation="MSDDAPTLALAAGFAPATDAAWRTLVDKVLKGGDFERRLASRTA
                     DGLRIAPLYKRGDAPDVAVPAPGADAAGWDIRQRHGVADPAATNAAILADLAGGVTSI
                     ALQIEAPGQTGAGYTADVLRRALAGVLLDVCPIALDAGEYTVDAAGSLMEIWREQGIA
                     AGRCRGAFDYDPLGTLARTGALYNPLPKALATAAHLITDTASMPGVTALVADGRPYHA
                     AGASEAQELAAVLATLVAYLRAAEASQIAPVTAIKKISIALAADQDQLLTIAKLRAAR
                     LLAGRIGEACGVPDAGRIHITAETAFRMMARRDPWVNMLRTTIACASAALGGADAITV
                     LPFTAALGAADAFAHRIARNTHHVLMAESGLGRVGDPAAGSFALDAMTGELSKAAWGL
                     FQEIEREGGMAASLTAGAFQGRIV"
     assembly_gap    11226..11252
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11253..11839
                     /locus_tag="PK264_06445"
     CDS             <11253..11839
                     /locus_tag="PK264_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRD75563.1"
                     /translation="IATGRLELTGVSAFPMLGPDGVMVEPSPTAPSADLNGAKAQRLE
                     ASRLAEPYEALRDAADAYAQRTGKPPRIFLASLGDLATHGTRSTWIANYLAAGGIEAI
                     RSDGFTASQDAARAFAESGAAIACICSSDAVYAELGEATAGALKAAGARRILLAGRPG
                     SIEAALKAAGVDTFIFAGSDAISTLRELHVALDA"
     gene            11951..13851
                     /gene="dxs"
                     /locus_tag="PK264_06450"
                     /pseudo
     CDS             11951..13851
                     /gene="dxs"
                     /locus_tag="PK264_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            13868..>14053
                     /locus_tag="PK264_06455"
     CDS             13868..>14053
                     /locus_tag="PK264_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRD75564.1"
                     /translation="MIVEAAPAKLNLFLHVTGRRADGFHLLESLVAFVDIADEVRIEP
                     AGRFVLEIVGPFAGALAD"
     assembly_gap    14054..14063
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14245..14829
                     /locus_tag="PK264_06460"
     CDS             14245..14829
                     /locus_tag="PK264_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75565.1"
                     /translation="MRTSRCTRTTARSATATSGATPRLAEVARSLGQDVPVCLAGTPA
                     YFEGIGDYTRPSPPLPRLSVVLVNCGTPVATPAVFRARNGAFSAPLPGNLDASSPEAL
                     IEALAACRNDLTDAALTIDTQIAVVLRELAACTGARIARMSGSGGTCFAIFDDETAAR
                     AAARNLTLAHAGWWIACGRLISLPRAAPRDTSAA"
     gene            14826..15710
                     /gene="mazG"
                     /locus_tag="PK264_06465"
     CDS             14826..15710
                     /gene="mazG"
                     /locus_tag="PK264_06465"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRD75566.1"
                     /translation="MTPSRDIQRLIEIMVALRTPVTGCPWDLEQSFRTIAPYTIEEAY
                     EVADAIERRDLADLKEELGDLLLQVVYHARLAEEEDAFSFADVVLGITEKMIRRHPHV
                     FGDEAARSAGIAKGFWERIKAEEKAGKARERVRLGAAGKGEAQQAADSLLADVPASLP
                     GLTRAVKLQEKAARVGFDWPSLAPVFDKLREEVEELSEVALAADPRGASEDQQGPALK
                     DRIEDELGDLLFVTANVARHLAIDPEAALRRANSKFLRRFRHIEMRLAEQGRAPEHST
                     LDEMEALWIEAKAAERKP"
     gene            complement(15727..16149)
                     /locus_tag="PK264_06470"
     CDS             complement(15727..16149)
                     /locus_tag="PK264_06470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD75567.1"
                     /translation="MDAEQVSELLRTHFPGFTADGSKVAIEAATPTHARVRMIPTPST
                     IRPGGTISGPAMFKLADLAVYVALLSELGVAGLDTATSSLNITFLARPEPRDMIADAR
                     ILRRGRRLVVGEVEMVTEGKPDLVAHAVVTYVMPAGPA"
     gene            16271..16792
                     /locus_tag="PK264_06475"
     CDS             16271..16792
                     /locus_tag="PK264_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75568.1"
                     /translation="MRRLVALVGALALAVTVALGLGSASAQEPIKQIKLTEKQVLGFI
                     ASQKEMMAIQVKLQASPADKPDPKILAEFEAASKKHGFASFAEFQDVTANISIIMAGF
                     DPATGKFTDPLEMIKAEIAGITADKNIPDKERKQMLDELNEALKITEPLKFPENIEIV
                     KKFREKIEASMQQ"
     gene            16960..17664
                     /locus_tag="PK264_06480"
     CDS             16960..17664
                     /locus_tag="PK264_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRD75569.1"
                     /translation="MAQRPIDLYYWPTPNGFKITIMLEECGLPYTIRPVNIGKGEQFK
                     PEFLAISPNNRMPAIVDPDGPGGKVISVFESGAILQYLGRKTGKFYPADERARVEIEQ
                     WLFWQMGGLGPMAGQCHHFRNYAPEKVPYGIERYTNEVNRLYGVLDRRLADRDFIAGP
                     YSIADMASFPWVRPYKNQGQDLAEFPNVDRWFKRMIARPAVAKGCKVGEELRSAYNLA
                     TDKEAQAVLFGQKARK"
     gene            complement(17688..18941)
                     /locus_tag="PK264_06485"
     CDS             complement(17688..18941)
                     /locus_tag="PK264_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75570.1"
                     /translation="MAIAIAGFGDDAKHVRLSEAELVTRVVQDALGAAGQVSVTRLDD
                     AARLLAIARFANPGGAMKQIDEAMKSHKADLFLFASDVHRNADGAVDISLDVMTFPDR
                     ACTGSKLIRGLKLPVSVARPETVLMAAARQFLRTEKQSARLVIQPTRLDGRPLTAKSG
                     RWFEEQLKAALVSAQPFSLGDPVTLEIAIARDTPMAGDADDKTWRAEVNLVSEPQHVA
                     LLVAFSKAGQTTGAGPEPIATNDLRNGLDIENALLTVTALKRRIAAPPKPGSGETLRF
                     EIKFARDLNLYCFADVPSKQGSYAIFPPRNGSSLLAATSSPVRYPEGLKIPPDLLNAD
                     EPGRTRLTCFGLAKPPPKSVLDAWKPATLQARLDVITAKGIPETSPDFDRHLMLSPTE
                     RAALAKLLKDAGAEEAGAEVEIVKP"
     gene            complement(19281..>19990)
                     /locus_tag="PK264_06490"
     CDS             complement(19281..>19990)
                     /locus_tag="PK264_06490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRD75571.1"
                     /translation="VASGEQVTAGLTALALQQANGIADPTKLRPGMVLKVPVVAAAAP
                     PAAPASPAPISPAPVATSPIASPKPASPEPKRVAALGTVPTTSPVISDASPAPAPASA
                     APTVSTATPKFRWPVKGRVVAAFGPRPDSTHNDGINIAVPAGAEVVAAEGGMVAYAGN
                     ELKGYGNLVLIRHENNWVTAYAHNDEILVKRGDKVKRGQPIARAGRTGADGQPHVHFE
                     VRQGSRPVDPIPYLEGN"
BASE COUNT         3174 a         7032 c         6555 g         3192 t
ORIGIN      
        1 cgcacatagc cggaggggcc attgcgatcg acggcctcca ggcccgcatg cgcctcgtgg
       61 gagaagatgg tggccagcgc ctccggtgac gtcgctccca tgccgttgaa gcgggcgatg
      121 gtgccgtcgc cgagccggag atggccgagc atgccgcgcg cacccgagat tgcctccgcg
      181 agctcgtccg gcactggctg ctggcgtgcg agaaagcact gccgaagcgg cacgagatcg
      241 agcatgatct cgaccaggac acccgggttg cgggtctcgt gaccgccgtc ggcgagcacc
      301 tggcgcttaa gctcggcgcg gagcgtgcgc acgtcgcgct cgaggcgctc gtcgtgtccg
      361 gcaatgcaca ggtccgccgt cacgagagcc gcaatcgcga ggaggcgcgg atagcccggc
      421 ggcgaatcgt gaaggctcgc tgcaagatgc acgatctggc gcccgaggct gtcggtcagg
      481 aaggtgtaat catcggcact cgtgtcatcg agcagaaagc ccgcatgcgt catccaggag
      541 aggacgcgcc tcgcaacgac actggtctcc caggggacgc cgcggtgcgc cgcatagcgc
      601 ccgatccact cgcgcaccat gccgcgcgca atgaggcgcg tctcgtcact ctgcgcagcg
      661 cgcaggtgcc gcagccaggc aaagccgtgc agctcccgga gccaggcagc actcggcggc
      721 gtcacctcga atgcgcttgc ccgcgaaatg tcggccacgg cctccgcaag accgagctga
      781 ccgtcctcca gctcgcgcac gaagctcgga tcagcgatcc tcacgtccgg cggcagaatg
      841 agcatctggt cggcccgcgc cgagcgctgg ccccagccga tcagcggcgc ccgcttcatg
      901 gaggcaagtg cggcgcggcg ctgccggtcg gcctcgatga tcgcgagccg catgcgttcg
      961 ccgaacgtca gccctccggc catatcacgg gtccccgccg cgtcgacgtg tcggggcaga
     1021 acgggtcatt ccgccggtcg cgacgtcaaa ccacgcggcc tccgggggat agccgcctcg
     1081 gtcggggcat gcaaggcagc gtggcgcgct ctcccgccta tttgtggatc atccgatatc
     1141 ggacgggcgc gcctgtcaac ccttcggcgg cagaaaggat ggcgttcacg tcgagaccat
     1201 agtgggcgaa gagctcaggg atcgagcccg actggccgaa ggcttcgacg cctagaccgc
     1261 gcacgcggtg cccgagcacg ccgccgagcc aggtcagtgt ctctggatag gcgtcgcata
     1321 ccgtgatcat ggcggcatcg cgcggcatgc ctgacagcaa ccgctccaca tggctcgtat
     1381 agccgacgcc tcgcgaagga cgagttcggt cagctcgcag acgtcctctc cgcgctcgcg
     1441 cgccttctgc gccgcatgcc accccgccga cagccggtcg gcgctcgtca ccgcgagcac
     1501 gccgacgccg cgccggtcct cggcaagaag cccggcggcc tcaattgcct cgggtgccac
     1561 agcgcccatg tagacgatga ccaccgacga cgacgtatcc ggctcgcgca cccagtagcc
     1621 gccatcgatg atcgcctgcc gcagctccgc cgtcatggca cgggccggct gctcgagtgg
     1681 acgcgtcgtc agccgcagat agaccgatcc gcccatttcc tcgcgcggcc acggcacgtc
     1741 cttccccttg ccgtcgcgct gcatgtagtc gagcgaccag cccatgatct cggcaagctc
     1801 gtccacatag gccggctcga aagagacgag gccatcctgc gacatgccaa tgagcggcgt
     1861 gccgatcgac tggtgcgcgc cgccctcggg cgagagcgcg atcccggacg gtgtcgccac
     1921 caccatgaag cgtgcgtcct ggtagcaggc gtaattcagc gcatcgaggc cgcggcagat
     1981 gaagggatcg tagagcgtgc cgatcggcag gagccgctcg ccgaatacag agtgcgagag
     2041 gccggccgcc gcgagttgca ggaagaggtt catctcggcg atgccgagct cgatgtgctg
     2101 ccctttgggc gacagctccc agcgctgcgg cgacatcagc ccgcgcttct tgaacatgtc
     2161 ctcctggctc tccatcgaga acacgcctcg gcggttgacc cagggcccga ggttggtcga
     2221 gacggtgacg tccggcgaca tggtgacgat gcggtcggcg agcgggccgc ccatgcgcgc
     2281 gatctcgtcg aggatgcgcc cgaagccggc ctgggtcgcc gccatcccct tggtctcgat
     2341 cttcggcatg acgaccggga gcttgggcga ggagagcgtg cgcttcccct tggcgatgaa
     2401 cggcacgcgc ttcaggaagg cggcaagcgt ctccgccggc aggtcgaggc ctgcgaagcg
     2461 gtcccattcc tcgccctggt tgatgccctg gcgcgcgcga aactcctgca tctgcgactt
     2521 gttcatcagt ccggcgtggt tgtccttgtg gccctggaat ggcaggccat agcccttgat
     2581 cgtgtaggcg atgaagcaca ccggctgatc agccggcgcg ccttcgaaag cctccaggat
     2641 ctcctcgaga tcatgcccgc cgaggttggt catgaggcgg ctcaagtcct cgtccgagag
     2701 tgcccggatg atggagagcg tgtccgtatg gcccgcgaga tcgcgctcga gctccgtccg
     2761 ccaggcagca ccgccctgga agacgagggc tgaatagagc tggttcgagc agtcgtcgat
     2821 ccactggcgg agccgctcgc cgccgggctt gcggaatgcg gcctgcatga gcttcccgta
     2881 cttgatgaag acgaccttcc agccgagatt gtcgaagagg ccggcaacct tcatgaacag
     2941 gcggtccgag acgaccgaat cgaggctctg gcggttgtag tcgaccaccc accagcagtt
     3001 cttgaggccg tgcttccaga actcgagtag tgcttcgtgg acgttgccct catcgaattc
     3061 ggcatccccc atgagcgcaa tgagacgacc cttcggccag tcgcggctcc agcccttggc
     3121 gtgcacatag tcctggatga tgctcgcgaa gcccgtgatg gcggggccga ggccgaccga
     3181 ccccgtcgag aagtcgacgt cggccgtgtc cttcgtgcgt gagggatagc tctgcacgcc
     3241 gccgaaggcg cggaagttga cgagcttctc gagctcctgg ttgccgagca gatactggat
     3301 cgaatggaac acggggctcg cgtgcggctt cacggcgacg cgatcctctg gccgcagcac
     3361 gtggaaatag agcgccgtca tcagcgcgac catcgaggcc gacgacgact ggtgcccgcc
     3421 gaccttcaag ccatcgccac gctgtacctc gtggttggcg tgatggatca tgtaggacga
     3481 gagccaaagc accttgcgct cgagctcacg cagtacagtg agtaactcct gccgacgagc
     3541 gtcgccaatg ggtgccagct gctgcatctg cgcctccgcg tgcggtccga tgggttccga
     3601 gttgccgcca ccatacgccc gctcatgcag cattgctttg caaaagcgcc cgctgacatg
     3661 caacatattg ggaggttttg ccaccaggag cgccaatatt gaagaaatcc accaatgtga
     3721 ccctggacga catcgatcgc cgtctgctca aggtggtgca ggaggagggg cgcatctcgg
     3781 caaccgaact cgccgagcgc gtcggcctct ccgtgagccc gtgcttgagg cgactccgcc
     3841 ttctcgagga ggcgggcgtc gtgcgcggct atgcggcaca agtcgatcag gcgaaggtcg
     3901 ggctgccggt gagcgtattc gtgtcgatca agctcgagcg ccagcagaag gacgccatgt
     3961 cccgcttcga ggccgccgtg cgccgctgcc cggaggtgct cgaatgctac ctgatgaccg
     4021 gcccgcgcga ctatctcctg cgcgtcgtcg cccgcgacct cgccgattac gagcgctttg
     4081 tcaaagagac gctcacgcgc ttcgacggca tcgccaacat cgaatcgagc ttcgcccttg
     4141 ctcaggtgaa gcagtccggc agcctgccga tcgaggggtg agccgcttgc gcttcgagct
     4201 ggtgacgacc tggttgccga attcgtcact tggccggagc gctcgcgcct gcttcattca
     4261 gcgtgccagc caatctggcg tctcctgcgc cccgccgaca ccaagaggcc cccttgccga
     4321 tccaatcgat cgcggacagc cgcgcgccgc tctggctcgc cgggatgctc gccgtcgcgg
     4381 caacggccct tgcggcggac cgcgtcctca tgagcgacct cggcggctat tcacacatgg
     4441 cgctggcgca cagcgtgctc tacctcgtga cggccgggct gctgctctcg agccgggctc
     4501 gcgccccctc gctcttcgcc attctggcga tcgcgctcgt catgcgcggc atcgccatgg
     4561 gcgtaccgca caacgtcctc accacggacg cgctccgcta cgtctgggac ggccgcatcc
     4621 agtgggcggg cttcaacccc tacctttacg cccctgccga cgagcggctc gcgttcctgc
     4681 gcgatcttgt gatcttccca aacatcaatc agaaggaaac ggcagtcacc atctacccgc
     4741 cgttcgccga gatgatcttc atgctcgccg tgaagatcgc cgacggcatc gagggcatga
     4801 agctcaccat gctcgccttc gagggactgc tcgtatggtc gctcgtcggc tgcctcgacc
     4861 ggcttggcat ctcgcgcgat cgcgtcctca tctacgcgtg gcacccgctg cccgtctggg
     4921 agttcgcgag ccaggcgcac atcgacgccg caaccgcggc gctcatcacg gctgcactct
     4981 atgcccgcct cgttcagcgc tattggctga cagggatgct cctcggcctc gctgccgcga
     5041 cgaagtacta cccggccttg ctgctcgccg tgctgctgcc gcgtccgccc tggctcgatc
     5101 gcctgctccg gcgcattccg ggtgttcgct cgctcattcc cgacgaggcg gcgggcgctc
     5161 gagatgagtg caggacacgc caatccctcc cagactggct gcagctcagc ctcggcttcg
     5221 tcgcgacgct gatcgccgtc tacctcccct atgcctggac ggccggcccg cgcgtcatcg
     5281 gcttcctgaa ccgccacctc gacaacgagg gctatctcgc aggctacggc ttccatctcg
     5341 tctggatcct gcgcgacttc ggcctcgccg acccgcccgc ccgcgcctac atcgccgccg
     5401 cggccgtgac cctcggcgca cttcttgcct gggccttcct gtcacgccgc gccagagatg
     5461 tgcctatccc gagcctcgtc gtgatcgcct gcgctttcgt ctggctcacc tcgccgcact
     5521 atgcttggta cttcgcctgg atcattccgt tcgcggcgct cgcgccttcc cccgcggccc
     5581 tcctcttcac ggctctcgca ttcgtcatgt acctccccac catcgcgccg gctcacgggc
     5641 agagcatcgc ctatgccctc gtcttcggag tgcccctggt cgtggcctcg gtcgagcgtt
     5701 ggcgcgcgcg cacacggctg cctgaaacgt gacgctcgtc ccgctgccct tccgctcgcg
     5761 cgccttccgc agccagtggc tcggcgatct cctcacgtcc tggagcttcg agatcgagcg
     5821 gctgatcctc ggctggtaca tcctcgtcga gacgaagtcg gtcgtgatgc tgaccctctt
     5881 cggcgcgatg cagtacggcg gcacgctcgt cgctcccctc gtcggcgtgc ttggcgaccg
     5941 gatcggcccg cgccgcgtgc tcgcgaccat gcgactctgc tatgcggtct gcgcgggcac
     6001 gttgctcgcg gtcgcctgga gcgggcggct gacgccgacg atcgtcctct tcgtcggcgc
     6061 gatcgccggg ctcgtcatcc cttccgacat cggcatgcgc ggcgcgctca tcgccgagac
     6121 gcagccgcgc gagcagctga tggcagccat gggcgccggg cgcacgacgt cggacacggc
     6181 gcgcatcggc ggcgcgctcg ccggcgcggg cctcatctcc gccttcggca tcgtgccgac
     6241 ctatttcgtc gtcacctgca cctatgtcgc gagcgcgctt ctcaccttgc gtgccgtcgc
     6301 cagcctgccg ctggaggctg gcccgcgccc ggagcgggca ccgacgtcgc cctggtccga
     6361 tctcaaggag gggctcgcct acgtgtggac gacgccgcac gtgctcggcg gcatggcgat
     6421 cgcctgcctc gtcaacctca cggcgttccc gatcatgggc ggcgtgctcc cctatgtcgc
     6481 caaggaggtc tacggcctcg gccagacggg cctcggctat ctctcggccg ccatcggcgt
     6541 cggcgccttc atcggctcga tcgcggtcgg catggccggc atgaggatcg ccgccggacg
     6601 cgtcatgctg atcgcaacca tcgcctggta tctgctgctg cttgctctcg ccctcgtcac
     6661 agacccgcgc gccggcgcgt tgctgctgat gctggtcggc ctcgcccaga gcttcagcat
     6721 ggtgccgctc gccgtcatgc tgctgcggac atccgagccg cgctttcgcg ggcgtgtcat
     6781 gagcgtgcgc atgctcgcga tctacagctt gcccttcggg ctcctcggcg ccggcgcgct
     6841 catcgaacgg ttcggcttca aggcgacggg cagcgcctac gcgctcctcg gcctcgcaac
     6901 gacgctcgcc attgccatcg cctggcggcg cgcgctctgg acggaccagg cgccgggaaa
     6961 tgcgcgctaa cgcttcacac cgatcgccgc gacgccgcgc tcgatccggt cgagcgcctc
     7021 atcgaggatc gcgcgcgagg tcgcgaggct cagccgcacg tgccccgcgg ctcccggacc
     7081 gaagaacctg gggctgccag gcacgagcgc gatgcgaaac tcgcgggcga gccgctccac
     7141 cacatcgtcc tgaccgagcg cgaggcggct cacgtcggga aatgcgacga acgtgccttc
     7201 cggcagcgca caggcgacgc ctggcatgcg attgagccgg gcaacggcga catcgcgctg
     7261 tgagcgcaaa tgctcgacga acgcggcccg ccaggctgcg ccgtgctcca gcgccgcgat
     7321 gcccgcgatc tgcgagagcg tgctcacccc atcggccgtc tcgtcgaaat gcgtcgtcga
     7381 taccagtcgc cggtgctgcc cggtatccgg cgagatcacg agccccaggc ggaggcccgc
     7441 catcccgtag cccttcgaga agccatagac cgtgaacgtc cgccgcgcga cctcgcccga
     7501 cagcgccgcg acggatgtca gcctggccgg cgcgaagacg atgtccgacc acacctcgtc
     7561 ggagacgatc cagagtttgt gcctgagagc caccgaagtc agcgcctcga gctcctcgcg
     7621 gcgccagacg cgccccgtcg ggttgtgcgg attgcagatc atgaggatgc gcgtccgcgg
     7681 cgtgacgagc cgctcgacgg ccgccacatc gaccgtccag cccgcggcct catcgagccg
     7741 gaagcggcgc accgtgccgc ctgccgccac gaccgagcgc tcgaacagga aatcgcacgg
     7801 gtccgcgatc aaggcctcct ctcccggcga cagcagcgcg cgcaccacga gatagagcgc
     7861 tgccgccgca ctgttggtcg ccagcacctc gccggctgtg caaggaatgc cgcggctccc
     7921 cttgaaatgg cgcgcggcag tctcgcggaa cgcgccgagc ccctcgccgg gaccgtagga
     7981 gaaatatccc gaggcggcgt agtcgcggat ggcctcgacg atctcgcgtg cggccgggaa
     8041 gtcggggtcc gcagccgtca gcgggatgac gtcaggctcg accgtcgccc agcggtagtt
     8101 gtgcgcccgc gcgcgcagca tggcgcgatc gacgcgatcg tcggaaaaga gcgtgttcga
     8161 gtcatcgccc ggttccgccg ccaccggcgc gctgccgaca ccttgagaca gctcggcgag
     8221 cgagaggcgg ccgtcgccgt cccgatcgag cgtatcgaac tgcactttga tctcagcctc
     8281 gctcacgacg cgccccgaca tggcaagcgc cagggcgaac tcgctccggt cgaggccgcc
     8341 cgccggcccg acggcgagcg tgcggaagag atcgacgatg cggctgcggc gctgatagag
     8401 cgcctggcgg cgaggcgagc ggccggcccg gaggcgggcg agtgcaggcg aggcggggcc
     8461 atccccggca agagctgcga ggatctcttc gacggagagc gagccgctcg tgtcggtatc
     8521 gatctcgctg aacaggccct cctcctcacc ggccgagatg tcggcctcga agagcgcaag
     8581 cgcacgcccg aagtcctgcc ggccgagccg tccgtcgcca tcgacgtcgg ccgccaagaa
     8641 ggccgcgcgc agatcggcct cgctcagctc gaccagatcc tcgatcggcg tcgcggtgcc
     8701 gtcgataagg gggacgccac cggggatcgc tgtgccgatg aagctcgcga gcatcgcgcg
     8761 gccgccgagc acgaagccct cgaccgccgc ccgccggtcc gtgacgaagc gcgcgagcac
     8821 ctcgggaacg cttccggtcg ctgtctcgga ggcggcgagc cggtccgcga gaatgatcgc
     8881 atcctcgagt gcagcgccga tcccctggct cgtgaacggc agcagggggt gggcggcgtc
     8941 gccgacgagc acgccgttcc cgcaggcgag ctcggcgggg aggtcggaat tgggcggatg
     9001 ccagacatgc gcggtcgcgt agtcggcccg ctcgaggtat tgccgcacca cctccggcgc
     9061 gccagcaagc agccggccta gaaatgacct tagcgccgcc gcgtccgcat cctgagtggg
     9121 cggcgcatgc cggcgcgcat cgaactgcat gaaaccgatg acgcgctcgg cggttggtgc
     9181 gagcaggccg aacgccacac cgcggtcggc gaacatggtc ttgatgaagg tcgtaccgag
     9241 ctggcgcgca agctcgggga ggtacgtcga ggtgacgatc tcgcggaccg ggctcagggg
     9301 ccgctcgaac ccaggattga gcgcccggca gagcggcgag cgccagccct cggcgccgat
     9361 cacgatgtca ccctcgccga ggtcgatcga cggccggtcg gcgagcacga tgcgcgagac
     9421 ccgctcgccc tcggcctcga tcctgtcgac tgcatggccg agcttcacga cgcccgccgg
     9481 gagccgtgcc gagagcgcct cgatgattgc cgagcgcgtc acgcagtaga cgccgtccag
     9541 cgcgccgaca tcgagcgagc ggccacccgc gtcctggatc agcgcgcgcc gtatcgccgt
     9601 gcagctgtcg agcacgcggc cggcatctag cgcgcgcaag gccttggccc cgttctgcat
     9661 gaggatcagg ccatgcccct tgcgggttgc ctggcgctcg gcgctgtcgc gttccaggac
     9721 ttccacgtca gtgatgccgg cgcgccggat gagggccgtc gctaccgcca gtcctgcgac
     9781 gccgccgcca agtatcttca cgcgcatccc gaaatcccct gtggaccggc ctcgcggcgg
     9841 gcaagaacgc tcaattcgac gagcatcttg ccaagccggc gtccacacgt caccaggaag
     9901 catccgacga aggggccatt tcaaccgatg gccggttggc tattgtgctc cctgtcgccg
     9961 ttccgagaga ccgtcccatg tccgatgacg ccccgaccct cgcccttgca gccggctttg
    10021 ccccggcgac ggacgccgcc tggcgcacgc tcgtcgacaa ggtgctgaaa ggcggcgatt
    10081 tcgaacgccg cctcgcctcg cgcacggccg atggcctccg aattgccccg ctctacaagc
    10141 ggggcgatgc gccggatgtg gccgttcctg caccgggcgc ggatgctgcc ggctgggata
    10201 tccgccagcg gcacggcgtc gccgatccgg ctgccaccaa cgcggcgatc cttgccgacc
    10261 tagcgggcgg cgtcacgtcg atcgcgctgc agatcgaggc gccgggacag accggcgccg
    10321 gctatacggc cgacgtgctc cgccgcgcgc tcgcgggcgt tctcctcgat gtctgcccga
    10381 ttgccctgga tgccggcgag tacaccgtcg atgccgcggg cagcctgatg gagatctggc
    10441 gcgagcaggg catcgccgcc gggcgttgca ggggcgcctt cgactatgac ccgctcggca
    10501 cgctcgcccg gaccggtgcg ctctataatc cgctgccgaa ggcgctggcc accgccgccc
    10561 acctcatcac cgataccgcg tccatgccgg gcgtcacggc cctcgtcgct gacgggcggc
    10621 cctatcacgc ggccggcgcc agcgaggcgc aggagctggc tgctgtgctg gcgacgctcg
    10681 ttgcctacct ccgcgctgcc gaggcctctc agatcgcgcc cgtcacggca atcaagaaaa
    10741 tctcgatcgc gcttgccgcc gaccaggacc agctgctcac catcgccaag ctccgggccg
    10801 cgcgcctcct ggccgggcgc atcggcgagg cttgcggcgt gccggatgcc ggccgcatcc
    10861 acatcacggc ggagacggcc ttccgcatga tggcgcggcg cgatccctgg gtgaacatgc
    10921 tgcgcacgac gatcgcttgc gcctctgccg cgctcggcgg cgcggatgcc atcacggtgc
    10981 tgccgttcac agcagccctc ggcgcggcgg atgccttcgc ccaccgcatt gcccgcaaca
    11041 cacaccacgt gctgatggcg gaaagcgggc tcggccgcgt cggcgatccg gcagcgggaa
    11101 gcttcgcgct cgacgccatg acgggcgaac tctccaaggc ggcctggggc ctcttccagg
    11161 agatcgagcg cgagggcggc atggcagcct cgctcacggc gggcgcattc cagggccgca
    11221 tcgtcnnnnn nnnnnnnnnn nnnnnnnnnn nnagatcgcg acgggccggc tcgagctcac
    11281 cggcgtctcg gcattcccga tgctcggccc cgatggcgtc atggtcgagc cctccccgac
    11341 ggctccatct gccgacctca atggagccaa ggcgcagcgc ctcgaagcca gccggctggc
    11401 cgagccctac gaggcgctgc gcgatgccgc cgacgcctat gcccagcgga ccggaaagcc
    11461 gccccgcatc ttcctcgcat ccctcggcga tcttgcgacg cacggcaccc gctccacctg
    11521 gatcgccaac taccttgcgg ccggcggcat cgaagcgatc cgctccgacg gcttcacggc
    11581 gagccaggac gccgcccgcg cctttgccga aagcggagcg gcgatcgcct gcatctgctc
    11641 gtccgatgcg gtctacgccg agctcggcga ggcaacggcc ggcgcactca aggccgcagg
    11701 tgccaggcgc atcctccttg ccggccggcc gggctcgatc gaagcggcgc tcaaggctgc
    11761 gggcgtcgac acgtttatct ttgccggatc ggacgcgatc tccacgctcc gcgagttgca
    11821 tgtcgcgctc gacgcgtgag cccgtgtgcc cgaaccggcc gaaagttgcg gacttttgcc
    11881 gcaagctcgg gctgtgaggc gagcccgggc tcgtgctact ttgccaggac gatgaaatga
    11941 ggactgtcgc gtgaccggct ccaagacccc cctgctcgac aaggttgccc ttccggctga
    12001 cctaagggcc ctcgacgaca agcttctgcc gcagctcgcc gccgagctcc gccaggagat
    12061 gatcgatgcc gtgtcgatca ccggcggcca cctcggcgca gggctcggcg tcgtcgagct
    12121 caccgtcgcc ctccacaagg tgttcgacac gccggacgac cgcctcgtct gggacgtcgg
    12181 ccaccaggcc tacccgcaca agatcatcac cgggcggcgg gaccgcattc gcacgctgcg
    12241 ccagggcggc ggcctctcgg gcttcacgaa gcgcgacgag agcccctatg atcccttcgg
    12301 cgcggcgcat tcctcgacgt cgatctcggc cgccctcggc atggccgtcg cccgtgatct
    12361 caagggagag gcacgcaaca tcatcgccgt gatcggcgac ggggccatgt cggccggcat
    12421 ggcctacgag gccatgaaca acgccggtgc ccggcacgag cgcctgatcg tcatcctcaa
    12481 cgacaacgac atgtcgatcg ccccgccggt cggcgccatc tcggcctatc tcgcccgcct
    12541 cgcctcctcg cagccctact tcttcatgcg cgatgtggca aagcagctcg ccaaacgcct
    12601 gccgaagagc tgggaacggg ccgccgcccg cgccgaggag ctgacgcgca cctactggac
    12661 cggcggcacg ctcttcgagg agctcggctt ccactacgtc ggaccgctcg acggccacaa
    12721 cttcgaccat ctgctgccgg tgctccgcaa cgtgcgggac gcgagcaagg ggccgatcct
    12781 cgtccatgtc gtcaccaaga agggcaaggg ctacgcgccc gccgaggcgg ccgcggacaa
    12841 gtatcatggt gtcaaccgct tcaacgtgat caccggcgcg caggacaaga ccaaggccaa
    12901 cgcgccccag tacacgaaag tgttcgccga gagtctcatc aaggaggcgg cgcgcgatcc
    12961 tcgcatcgtc gccatcaccg cggcgatgcc gtccggcacc ggcctcgatc tcttcgccaa
    13021 ggcctacccc gaacgcacct tcgacgtcgg catcgccgag cagcacgccg tgacgttcgc
    13081 ggccggcctc gcgacggaag gcatgcggcc gttcgcggcc atctactcga ccttcctgca
    13141 gcgcgcctac gaccaggtgg tgcatgacgt ggcgatccag cggcttccgg tccgcttcgc
    13201 gctcgaccgt gcgggcctcg tcggcgcgga cgggccgacg catgcgggct cgttcgatgt
    13261 cggctatctc ggcgcgctcc ccggcttcgt cgtcatggcc gcggccgacg agtccgagct
    13321 cgtgcacatg gtcgcgacgc aggcccgcat cgacgaccgg ccctcggcag tgcgctaccc
    13381 gcgcggggaa ggcgtaggag tcgagctgcc agtcgagggc cgtccgctcg agatcggccg
    13441 cggccgcatc gtgcgcgaag ggacctcgat cgccatcctc tcgctcggca cgcggcttgc
    13501 cgaggcgatg aaggccgcga gccagctgca ggccttcggg ctgtcggcga ctgtcgccga
    13561 tgcccgcttc gcaaagccac tcgatgaggc cctgatccgc cggctcgccc gcgagcacga
    13621 ggtgctgatc acggtcgagg aaggagcgac gggagggttc agtgccttcg tcatgcagtt
    13681 cctcgtctgc tcgacggcgg cctcaagttc cgcccgctcg tcctcccgga tgccttcatc
    13741 gaccacgaca agccggagaa gatgtacgag acggcgggtc tcaatgcctc cgggatcgtc
    13801 gcggcagcgc tctccgcgct cggccgcaag gtgatggcca acgaaggctg aaacggccgg
    13861 ctcagcgatg atcgtcgaag ccgccccggc gaagcttaac ctcttcctcc acgtcaccgg
    13921 acggcgcgcg gacggcttcc acctgctcga gagcctcgtt gcgttcgtcg atatcgccga
    13981 tgaggtgcgg atcgagcccg cggggcgctt cgtgctcgaa attgtcggcc cgttcgcggg
    14041 cgcgctcgcc gacnnnnnnn nnngagccga tcgcgagcaa tctcgtcacg cgcgccgccc
    14101 atgccctggc ccgcagcctc gggcgcacgc cggatgtcgt cgtgacactc gtgaaaactc
    14161 tgccgatcgc ctccggcatc ggcagcggct cgaccgatgc cgcagcgacc ctgcgcagcc
    14221 tcggccggct cgaggacgcg gatcgtgcgt acatcacgat gtacgcggac gacggcacgt
    14281 tccgcaacag ccacgagtgg tgccaccccg cgccttgccg aggtcgcgcg gagcctcggg
    14341 caggacgtgc cggtgtgcct cgccggaacg cccgcctatt tcgagggcat cggcgattat
    14401 acgcggccct cgcccccact gccgcgtctc tccgtcgtcc tcgtcaactg cggcacgccc
    14461 gtcgcaacgc ccgccgtttt ccgcgcccgg aacggcgcct tttccgcccc gctcccggga
    14521 aatctcgatg cgagttcgcc cgaggcactg atcgaagcgc tcgctgcctg ccggaatgac
    14581 ctgaccgatg ctgcgctcac gatcgacaca caaatcgctg tggtcctgcg cgaactcgcc
    14641 gcctgcacgg gcgcccgcat cgcccgcatg agcggctcgg gtggtacgtg ctttgcgatt
    14701 ttcgacgacg agacagctgc gcgagctgcg gcccgcaacc tcacccttgc ccatgccggc
    14761 tggtggatcg cctgcggtcg actgatttcc ttgccccgcg ccgcgccgcg cgatacctcc
    14821 gctgcatgac cccatcgcga gacatccagc ggctcatcga gatcatggta gcgctccgca
    14881 cgcccgtcac cggctgcccg tgggacctcg agcaatcgtt ccgcacaatc gcgccctaca
    14941 cgatcgagga ggcctacgag gtcgccgatg cgatcgagcg gcgcgacctc gccgatctca
    15001 aggaggagct cggcgatctc ctcctccagg tcgtatatca cgcacgcctc gccgaggagg
    15061 aagacgcctt ctcgttcgcc gatgttgtac tcggcatcac ggaaaagatg atccgccgcc
    15121 acccgcacgt cttcggcgac gaggcggcac gctcggccgg catcgccaag ggcttctggg
    15181 agcgcatcaa ggcagaggag aaggccggaa aggcgcgcga gcgggtccgg cttggcgcgg
    15241 ccggcaaggg cgaggcgcaa caagccgcag actcgctcct tgccgatgtt ccggcatcgc
    15301 tgccgggcct aacgcgcgcg gtgaagcttc aggagaaggc cgcacgcgtc ggcttcgact
    15361 ggccctcgct cgcgcccgtg ttcgacaagc tccgcgagga ggtcgaggag ctttccgagg
    15421 tcgcgctcgc tgcagacccg cgcggcgcca gcgaggatca gcagggcccc gcgctcaagg
    15481 accgcatcga ggacgaactc ggtgatctgc tgtttgtgac agccaatgtc gcgcgccacc
    15541 tggcgatcga tcccgaggcg gcgcttcggc gcgccaactc gaagttcctc cgccgtttcc
    15601 ggcacatcga gatgcgcctt gccgagcagg ggcgtgcccc cgagcactcg acgctcgacg
    15661 agatggaggc gctgtggatc gaggcaaagg ccgccgagcg caagccctga ccaacgtgta
    15721 cgcgcttcag gcagggcccg ccggcatcac ataagtgacg acggcatgag cgacgagatc
    15781 gggtttcccc tccgtcacca tctcgacctc gccgacgacg aggcgccggc cgcgccgcag
    15841 gatccgggca tccgcgatca tgtcgcgcgg ctcggggcga gcgaggaagg tgatgttgag
    15901 gctgctcgtc gccgtgtcga gcccagcgac gccgagctcg ctcagcaggg ccacatagac
    15961 ggcgagatcg gcgagtttga acatcgccgg gccgctgatg gtgccgcccg gccgaatcgt
    16021 cgacggcgtc gggatcatac gtacgcgcgc atgcgtcggt gtcgccgcct cgatcgccac
    16081 cttgctgccg tccgcagtaa agccagggaa atgtgtccgc agcagttcgc tgacctgctc
    16141 tgcgtccaat ggagagctgg cttgtccagt attgctcatc cttattgctg tccattttgt
    16201 gagttgtgga tgggatgggg aaggcctaaa ggccttgctc gcgagtggca aggtttacga
    16261 ggaggcatga atgcggcggt tggtggcgct ggttggcgct ctagcattgg cagtgacggt
    16321 ggcacttggg cttgggtctg cgagtgcgca ggagccgatc aaacagatca agctcaccga
    16381 gaagcaggtt ctcggcttca tcgcgtccca gaaggagatg atggccattc aggtgaagct
    16441 gcaggcctca cccgcggaca agcctgatcc caagatcctc gcagagttcg aggcggcgtc
    16501 gaagaagcac ggtttcgcgt cgttcgccga attccaggac gtgacggcca acatctcgat
    16561 aatcatggcc ggcttcgatc cggcgacggg caagttcacc gacccgctcg agatgatcaa
    16621 ggccgagatc gcgggcatca ctgctgacaa gaacatcccc gacaaagagc ggaagcagat
    16681 gctcgatgag ctcaacgagg ccctgaagat caccgagccc ctcaagttcc ccgagaacat
    16741 cgagatcgtg aagaagttcc gcgagaagat cgaagcatcg atgcagcagt aagatggctg
    16801 catcctgaga gacactccgg ggcgccgcga ggcgcccact ttttttgccg acgccgtatc
    16861 acaaggagag caaaccaccg ccagccgaag gcacggtctg gtgctctggt cgcaggcccg
    16921 tgtatgctcg ctcacatccc cctcgacacg gagcatgcca tggcccagcg cccgatcgat
    16981 ctctactact ggccgacgcc caacggcttc aagatcacca tcatgctgga ggagtgtggt
    17041 ctcccctaca cgatcaggcc ggtcaacatc ggcaagggcg agcagttcaa gcccgagttc
    17101 ctggccatct cgccgaacaa ccgcatgccg gcgatcgtcg atcccgacgg gccgggcggc
    17161 aaggtgatct ccgtcttcga atcgggcgcg atactccaat atctcggccg caagaccggc
    17221 aaattctacc cggccgacga gcgcgcccgc gtcgagatcg agcagtggct gttctggcag
    17281 atgggcgggc tcgggcccat ggccggccag tgccatcact tccgcaacta cgcgcccgag
    17341 aaggtgccct acggcatcga gcgctacacc aacgaggtca accggctcta cggcgttctc
    17401 gacaggcgtc tcgctgaccg cgacttcatc gccggtccct attcgatcgc cgacatggcc
    17461 tcgttcccct gggtgcgccc ctacaagaac cagggccagg acctcgccga gtttcccaac
    17521 gtcgatcgct ggttcaagcg catgatcgcg cggccggccg tcgccaaggg ctgtaaggtc
    17581 ggcgaggagc tgcgctcggc ctacaacctc gccaccgaca aggaggcgca ggccgtgctg
    17641 ttcggccaga aggcgcggaa gtagcagctc cgcaaccggc ccggcgacta cggcttgacg
    17701 atctcgacct cggcgcccgc ctcctcggcg ccggcatcct tcaggagctt ggcgagggcc
    17761 gcgcgctcgg ttggagagag catcaggtgg cggtcgaaat cggggcttgt ctcgggaata
    17821 cctttcgctg tgatcacgtc gaggcgggcc tgcagcgtcg ctggcttcca ggcatcgagc
    17881 accgacttgg gcggcggctt ggcgagaccg aagcaggtca ggcgggtccg gcctggctcg
    17941 tcggcgttca ggaggtcggg tggaatcttc aggccctccg ggtagcgcac cggcgagctc
    18001 gtcgcggcca gcagcgagga gccgttgcgt ggcggaaaga ttgcgtagga gccttgcttg
    18061 ctcggcacat cggcaaaaca gtagaggttg aggtcgcgcg cgaatttgat ctcgaagcga
    18121 agggtttcgc ccgagccagg cttcggcggg gccgcgatcc gccgctttag cgccgtgacg
    18181 gtgagcagcg cgttctcgat gtcaagccca ttgcggagat cattggtggc gatcggctct
    18241 ggcccggcgc cggtcgtctg accggccttg ctgaacgcga ccaggagcgc gacgtgctgc
    18301 ggctcgctca cgaggttcac ctccgcgcgc cacgtcttgt cgtcagcatc gcccgccatc
    18361 ggagtgtcac gcgcgatggc gatctctagc gtgacaggat cgccgagcga gaagggctgc
    18421 gccgagacga gcgccgcctt gagctgctcc tcgaaccagc ggccggactt cgcggtgagc
    18481 ggtcggccgt cgaggcgggt cggctggatg acgaggcgcg ccgactgctt ctcggtccgc
    18541 aggaactggc gcgccgccgc catcagcacc gtctccggcc gcgccacgct caccggcagc
    18601 ttcaggccgc gaatgagctt agagcccgtg caggcgcgat ccgggaatgt catgacgtcg
    18661 agcgagatgt cgacggcgcc gtcggcgttg cgatgtacgt ccgaggcgaa caggaagagg
    18721 tccgccttgt gggacttcat cgcctcgtcg atctgcttca tcgcgccacc ggggttcgca
    18781 aagcgcgcga tggccagaag gcgtgcagca tcgtcgaggc gggtcaccga gacctgtccc
    18841 gcggcgccga gcgcatcctg gacgacgcgc gtgacgagct cggcctcgct cagccgcaca
    18901 tgctttgcat cgtccccgaa cccggcaatg gcgatcgcca cgcgtcgttg ccacataccc
    18961 tcgaatgccc cgcgcgggcg tgtcttggca cgccgtgatc tcgcccttga tctgattggc
    19021 gagctgagcc gtgcccgcgc gcaacgccag ttcccaggcc ggagactcgg cgcgagcggg
    19081 aatcgccggc gatgcggtga gcgcaatgcc gatcggcaag gccgccacaa cggcggaacg
    19141 gcgggcgaaa gcgagcatgc aacctccaac atcgaccgtc tcgggcaacg cggtcgatga
    19201 tagccgcgcc ccagccaaac gcaatcgagg ccgcggttga tccgcagcct cgttggcttc
    19261 cgcaaccaaa cacgacagac tcagttgcct tcgagatagg ggatcggatc gacgggacgc
    19321 gagccctggc gcacctcgaa atgcacgtgc ggctgcccat cggccccggt ccgtccggcc
    19381 cgcgcaatcg gctgcccgcg cttcaccttg tcaccgcgct tcacgaggat ctcgtcgttg
    19441 tgggcgtaag ccgtgaccca gttgttctcg tgccggatga ggacgaggtt gccgtagccc
    19501 ttgagctcgt tgcccgcgta ggcgaccatc ccgccttctg ccgcgacgac ctcggcaccg
    19561 gcggggacgg caatattgat gccgtcgttg tgcgtgctgt cggggcgcgg gccgaaagct
    19621 gccaccacgc ggcccttcac gggccagcgg aacttgggcg ttgccgtcga cacggtcggt
    19681 gcggccgatg ccggggcggg cgcagggctc gcatccgaaa tgaccggaga tgtggtcggc
    19741 accgtgccga gagccgcgac gcgcttcggc tccgggctcg cgggcttggg gctcgcgatc
    19801 ggcgaggtcg cgactggtgc aggcgaaatc ggagcgggcg atgcgggcgc agccggcgga
    19861 gccgcagcgg cgacgaccgg taccttaagc accatgccgg gacggagctt ggtcggatcg
    19921 gcaatgccgt tcgcctgctg cagggcgagc gccgtcagcc cggcagttac ctgctcgccg
    19981 ctggcgacga
//
LOCUS       DAORNH010000042        19416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2172_length_19416_cov_2.952585, whole genome shotgun sequence.
ACCESSION   DAORNH010000042 DAORNH010000000
VERSION     DAORNH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19416
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2172_length_19416_cov_2.952585"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PK264_06495"
     CDS             <1..1080
                     /locus_tag="PK264_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75572.1"
                     /translation="GWLLASQLALLAAIVFLATRDPATATLSIGLGALLVAAASATQD
                     IVIDAFRIESLDPDQQAAGMASYVAAYRVGMLVSGAGVIGVTAWLEAKGYSKGTAWTL
                     AFVLAGATLVVGMLATLLSREPAQTSASLGTVAARADRGTLLARFLGTAIGAFTSFLT
                     RKGAIVALLFVLLYKLCDAVAGVMTGPFVLSLGYDKATYAGIVKGVGLAATLIGGFAG
                     GLVARALPLVAALWLGAILQMASNLVFVWLGTVTPSVGALTVAIVAENLAGGIGTVIF
                     VAYLSALCSSPAHTATHYALLTALASTGRTFIAAFSGYAAKSLGWPGFFLMTVVLALP
                     SLAVLVWLIRGGHFAALGGERRDGA"
     gene            1070..2545
                     /locus_tag="PK264_06500"
     CDS             1070..2545
                     /locus_tag="PK264_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HRD75573.1"
                     /translation="MAREAAPLQAIAGFVQDEEGHWVALLACGHRQHMRHVPPFIERP
                     WVATEQGRVAKFGTAIPCSLCLDDFSSVYARSSLDASRTMDEARVMTNRLPLDGIRVL
                     DVSSFIAAPAAAVVLGDYGADVIKVEAPGEGDPHRTNIQLASFPKAEVNYPWHLDARN
                     KRSVALDLKSKDGRAALDRLIESADVMITNFPFPVRDRLTLGYEAVAKVNPKLIYASF
                     SGYGEEGDEKDQLGFDANAFFGRAGILDQARYEGQPPAFALPAQGDRCSAMSLVTAIM
                     IALFDRARTGKGTWVASSLYANGLWSNGVHAQAALLGSYLPPRPPRDRPRSAVANIFV
                     TKDDRWMQVSVVREDKMWSTFLTAIERPDLASDPRFAETAIRRKNAALLTTILDPIFK
                     GRTWAEWHAILLRHNLPHSPIARAEDISVDPQAVAAKAIVPTAIAEMPRTIAAPFQVG
                     GHEPRKAGPGPGLGAHTDEILRAAGYSVADIAAMRKSGAVG"
     gene            2603..3922
                     /locus_tag="PK264_06505"
     CDS             2603..3922
                     /locus_tag="PK264_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HRD75574.1"
                     /translation="MPDNEPSSRTTPKLVDDCFRIDGRRLTHGEAIALLKERAAPIAG
                     ETSVTLAEADGRVIARPIVAPRPIPMHANSAVDGYAFAWASYDRERGSTLPVVGRAAA
                     GRPMREAVPAGAAARIFTGAAMPAGTDTVVMQEDTATRAVDGRDCVVVPPGLKTRANA
                     RAAGEDVATGQKIFEPGHAIRPQDIAALASLGLAVVPVFDRLKVAIVASGDEIVRPDS
                     KRAGAIEVGQVYDANSPMLAALLRSPSIEVDDLGVLPDDAAKVRARLVEAAELYDVIL
                     TSGGASLGDEDHMVAAIDALGTRHMWQLAIKPGRPISFGQIGRAVIVGLPGNPVAVFV
                     CYLMYVYPLLRRLAGAPWPTPRRLSLPALFSFKGRKKGRREFWRGMLVDGPDGLGVDK
                     FMRDGSGLISSLRAADGLIDICEDQGDVAPGDFVDFISFSEFGLAGR"
     gene            complement(3932..4147)
                     /locus_tag="PK264_06510"
     CDS             complement(3932..4147)
                     /locus_tag="PK264_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75575.1"
                     /translation="MSELFQPQDPIGATVRADCDIVSGHLVARQRQRRLTTTIALVLA
                     CAIGGGVLYAAQPKPYPAHMTAWAFRV"
     gene            4394..4921
                     /locus_tag="PK264_06515"
     CDS             4394..4921
                     /locus_tag="PK264_06515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRD75576.1"
                     /translation="MTFDRLKSRNDQLMSRLAKIKGSTALTTARRSLALGAPPKDAVT
                     LEAPVPESRTASPAAEPEPEMAQKRRTPRRRGEIPCTIQFDGLRMTLSARIIDMSAGG
                     ARLHLPPAAKTAYGGVRGLPDRLTLQIKSDRMEYEGAIAWRSESEIGVRFVAPPRQMK
                     VQATAPTTSPVRSAR"
     gene            complement(4925..5305)
                     /locus_tag="PK264_06520"
     CDS             complement(4925..5305)
                     /locus_tag="PK264_06520"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD75577.1"
                     /translation="MIHFIEPPATGEPRPFSRAAKANGMVWVSGHSAPHDPARGVLRG
                     ATPQEEVRNALSEVARILGEAGSSLDLVVQMTMLISRREDYQACNEEYVRHFPKGLPA
                     RHTALFGVPSEARVAFACVALLPD"
     gene            6077..15832
                     /locus_tag="PK264_06525"
     CDS             6077..15832
                     /locus_tag="PK264_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75578.1"
                     /translation="MSLPANAGQTPDFNSSTVELRSVTRTVHSIQAHSPNDVIAHARV
                     SGAEFAVTDKGVLALRDARGELTILDWTAPPAGSNAEIHAAIAVRVAERLNRTIAADW
                     MSRPATGDGGHDAETSDAGGKAPTPPPGLETPFQTRHSTLEGLADETRRWGRDDIKRA
                     EAGSAGAVLSTPVGAGLGHLVGLGDEDPGRRNGEKQSNEDGARFLGESVGVGSSIEHL
                     WLLGDFDYVRSALELSENADFLEPPASPNRPQYGPLTGPNPPSLMIEDTSFSGRLFDP
                     AMAGVPVVDRQITVDPRFGNAIFNIDGTFEFTPANGYSGPVTLEFSLTDPRTGRIETG
                     IIEIMVQAVIDAPTITSGTTTLEDTTVATPVSIILNDPDGSEVIDQVIITGVPTGAVL
                     SWDVRLPGLVSANPDGSLTLTGTTTEIQALLASLTLLPPDDFSGQITLNAAVVVTERN
                     LDPAIPGYLSSITINHPIVIDVVADADIPDVTGDERTTDEDTPVQLPSLAAALNDLDG
                     SEVLSIEVRNVPVGATFNVGQPDPVRPGVWLFTLAEIQAGPIFMPPANFIGDITMQIA
                     ATATEQANGDQEIVLDPIIVHVLPVDGPGVTVTPDITNEDTPVLIGDNITILIDDPLG
                     SETLTSVEIRGFPIGSVITYTDTAGIPQTVAITVAGTGILIAGGTEAQIRTAVATVSV
                     TPPQHSDGDLSIDVFATTTDIRGLTFTDQATLPVTVRAIIDAPTITSGATVLEDTTVG
                     IPLTVALTDQDGSEIINAVTISGVPTGAVFGWSKTLPGLVAANPDGSYTVTGTTAEIQ
                     ALLATLTLLPPDDYSGPITLNASVVINETNPDPGLPPGLATMTINHPIVIDVVADADI
                     PDVTGDERTTNEDTPVQLPGLTAALNDLDGSEVLSIEIRNVPAGAMFNVGQPDPVRIG
                     VWLFTLAEIQAGPIFTPPVNFIGDINMQIAAIATEQANSDQETVLDSVIVHVLPIDGP
                     DVTVTPDSTNEDVPILIGDNIAITIADPLGSETLTSVEVRGFPVGSVISFTDTAGNPQ
                     TVTITVAGTGVTIAGGTEAQIRDAVATVSVKPPPHSDGDFALDVFATTTDIRGLTFTD
                     QATLPVRVSAVADAPTVSGAGTGDEDTFIAVPITVTLVDADGSEVIDHVDISNIPAGA
                     TVQWTSAAGLVTTLPDGTLRITGSTAEIQARLASLEIRPPVDSDLNFDLAVLAEVREA
                     NPSEAGDVAVPIATRAFAAPVVVNPVADAPVITGTSSVNEDGTANPADPVTIVPVEFG
                     RNIAITATDSSDGSERMTAIRIAGLPIGAVVSYVPVGGGALQTFTVAAGITTLDLNGG
                     TEAEIRAALASLALIPPPHSDQDISLAITVTKSDATAIDTEPADVVQFVGTHVIAVAA
                     VADQPTLTTNGIGAGGIGFGLEDANVALSLTAGHPDSDGTERLREVLIRDVPAGFILT
                     ESSPGAGILALDAGRNAWVVTGPTDAAVNDVLANLTLALSPAVGGPRQHLDTDFTLNV
                     TVTSSELAPTNDATGGPEVALLDRSATFAVNIPVRAVADGVTPSGSSTLVEDRAATIG
                     PDLTWAHIDADGTEAVTRIEVSGFPVGATVAWTPAVGGPQLVVVPTGGTTIVLTGADI
                     RSALDTLAVTAPANSDANFSLTFDITTTDNDGSTIVDTHLHSVIVQAVADAPSALVAD
                     LSTSEDTPATLSINVGRSADDDAADNSETLSVRITVPSDVGGPVGVLTGTVPGGVTFT
                     SLGGGVYTVTAVGADPATREALLDSFLVGGISFEPRAQWSGVLSGTSGIRVDVTSTEA
                     AVGGELAPLGAEGAPNGTDGPTKTETVTRFIGIDVQPVVDAPTLANASTIIRENNNSS
                     AITDPDLVLALGSRLGLATLDTDGSQGLSLTLTGLPTNLQAASFAATFPDVTTSINLA
                     TGTIIVSSTSAVRALAVIGSLSITLADDDDRNFTVAINGTMSDTNGVASASSPFSLTH
                     DVIVRAVADTPTVTVGAATKPAVLEDSGFVGYPVTVALNDTDGSETFQSVRIDLSTPG
                     GGTRPVAQFAVTAGVTFDSSVPGRTVLTGSAADIANAMASLEVRPGADNGEDITVTVT
                     AIAAESNPAEDNNGATAGNGGGIAGPEISVPTAQTVRTFVIPVTPVVEAPTLNAPPTA
                     TGLEDMRNALAGISLSGIADPDGSEARFVEIDTTSFPAGSTFTSGGAPVGSIVSGWLR
                     IPEASLAALEVQPPLHFTGTIALSIRATTVDTTGTDVVTTSLAPQTLTVTINPVADPA
                     IVPADSLGFEDAVVAFGADLANVPTGIRVSDTVVGSPTNGGAETISAVALRVPADTSA
                     LTYTLSGTFVPSSAGTFAGSGTAEVNFNAATRTYTITSTIITGAADPATLTDAERSQA
                     EADIRATLASFQVAMGQDRDGNLVDDGQQDRNGLIDVTVTTLDVKNGVADTETTSFGH
                     DVVILARADAPAITAGFTASGNESGASTSIIPIVGASGERVVADRSIDTDGVGENSGW
                     GSEVLSVRISGLPLGASIVPVTGYTLPAGASLVPSGSNFVVNAANEDDLNDVLANIGL
                     VSPYYSGPATLTFTAITTEQGQAGEPVGGIDIAQATAVATVGITILPTVDTPIVKANA
                     SGLEDTIIAIPNAVTLGDPDGSESYVFRILATSVPAGAVIYGTGNVVIAEAGGYYTLT
                     AAEAAGLAIRPPTNFSTINPATPDIVLVTETIVSDGPAGPVTFTNSIAVAVQGVADQP
                     PAMPVTVSATEDEAYAIGAAIEATVLANTGGPLPSVLTDTDGSEVLSFVLAGLPPGVI
                     PQASAGTISYLGGGRWNVSADAVPTLTLPPVSDYSGNNPYPGLTLTAASQELDSDQAT
                     SAVWPLTINVAPVIAAATIDGFSSWSPDQTLTETAVETAGVSLASASIHDFADLDGSE
                     RVLRYVFNLSTLIADAEIAGRLAELPGAGSGLEKLVANYISGTFAYYAAPTVLTVNGQ
                     PLAVAAGSIVVLPADLASIALHPDLFLDSNVGFSIPVSALLEDTAGGPQYLMSTGLDV
                     TIDGIADVPTVFAANPDNDGNAVDVDTFTPLQFIPLSFGGVTTDIDDDRPGGPAGDGL
                     GRTQSEDVYYILSLVGTSGGPAPFLALVDSTTGQPAGLNNGDGTFLVRPGELANLALV
                     AADFSGPPVTMNFTVTTVAVDDASLATSAAPAAFSVIVDPGLGGTTGTPPPVPTVNLA
                     NLLGGTEDIVGALADPSQPVVTSDPSVQSTAVMFTVPVGATVAGAAWNPVTSRWVATA
                     ADFNAGLVTVQPTSREA"
     gene            15829..17535
                     /locus_tag="PK264_06530"
     CDS             15829..17535
                     /locus_tag="PK264_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HRD75579.1"
                     /translation="MTIIIEAVATGTNLIRATTGPIATSVYVDPVADGPSVTASPDPA
                     FEGAAFDLNIALAERDIDGSETLDGFAYVRLNNGAALVGVFPLVAIDDADATIDGQSL
                     VGFYRVPAPSLATLQALGAPGWHGSVNIEIAAVARDNSSPADPTPDADNTQLSLITVA
                     TAIAAVANAPIIPASIPTVSGNEDAAGGIPLSGLSAALDDVVTTNGGEQLSVVISGAP
                     LGTRFSSGTNNGDGSWTIPVSALATLRIIPPLDYSGRMSLSLNAVALELSNGDEATSS
                     VAFFVDVTPVADSVEILTPNVVVPAAGLAAVPMNVRMQDDSGLSALETAPELLRVTFT
                     GVPTGAFFVASHGGTLTEPTTGTWIFTGTEAQANALQVASGPNVAAGTSTVLISAVTI
                     DGASTLATPVLDSFELTFAAPSNPGTSVAGTGSGETLSGGAGNDVITGLGGGDTLSGL
                     GGIDRLQGGTGADNLSGGSGRDLFIWSTGDTVGGADTISDFTNGVDGDAIDVSALLVG
                     FNAQTAVLSEYLRLKPGDATTIQVDADGDIGGANFVDLATLTGATGLDVDAMRQNGNL
                     IV"
     gene            complement(17576..17651)
                     /locus_tag="PK264_06535"
     tRNA            complement(17576..17651)
                     /locus_tag="PK264_06535"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17616..17618),aa:Ile,seq:cat)
     gene            complement(17704..>18739)
                     /gene="eno"
                     /locus_tag="PK264_06540"
     CDS             complement(17704..>18739)
                     /gene="eno"
                     /locus_tag="PK264_06540"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRD75580.1"
                     /translation="QIHVDQTMIELDGTPNKSRLGANAILGVSLAVAKAAAEASGLPL
                     YRYVGGARASVLPVPMMNIINGGAHADNPIDIQEFMIMPVGAATCRDAIRMGAEVFHT
                     LKKGLSEAGHATNVGDEGGFAPNLKSADEALGFIMKSIEKAGYKPGQDVVLALDCAAT
                     EFFKAGRYAMEGEGKTLDPAGMAKYLADLVARFPIVSIEDGMSEDDWTGWKALTELVG
                     KKCQLVGDDLFVTNSERLAEGIKRGVANSILVKVNQIGSLTETLDAVNLAHGAGYTAV
                     MSHRSGETEDATIADLAVATNCGQIKTGSLSRSDRLAKYNQLIRIEEELGDVARYPGR
                     ASIKAGSAAA"
     assembly_gap    18740..18841
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18842..18980)
                     /gene="eno"
                     /locus_tag="PK264_06545"
     CDS             complement(<18842..18980)
                     /gene="eno"
                     /locus_tag="PK264_06545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969226.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRD75581.1"
                     /translation="MTAIVDIIGREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTG
                     AH"
     assembly_gap    19189..19198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3219 a         6603 c         6136 g         3346 t
ORIGIN      
        1 ggctggctct tggcgtcaca gctggcgctt ctggcagcga tcgtgttcct cgcgacccgc
       61 gatccggcga cggcaacgct ctcgatcggg ctcggcgcgc tgctcgtcgc ggccgcctcg
      121 gccacacagg acatcgtcat cgacgccttc cgcatcgaga gcctcgatcc cgatcagcag
      181 gcggcgggca tggcgagcta tgtcgcggcc tatcgcgtcg gcatgctcgt ctcgggcgcg
      241 ggcgtgatcg gcgtgacggc ctggctcgag gcgaaagggt attccaaggg cacggcctgg
      301 acgctcgcct tcgtgctcgc cggggcgacg ctcgtcgtcg gcatgctggc gacgctgctg
      361 tcgagggagc cagcgcagac aagtgcgagc ctcggcactg tcgcggcgcg cgcggatcgc
      421 ggcacgctcc tcgcccgctt cctcggtacg gcgatcggcg ccttcacgag cttcctcacg
      481 cgcaagggcg cgatcgtcgc gctgctgttc gtgctcctct acaagctctg cgatgcggtg
      541 gcgggcgtga tgaccggccc gttcgtcctc tcgctcggct acgacaaggc gacgtacgcc
      601 ggcatcgtca agggcgtcgg gctcgcagcg acccttatcg gcggctttgc cggcgggctc
      661 gtggcgcggg cgctgccgct cgtcgccgcg ctctggctcg gcgccatcct gcagatggcg
      721 tcgaacctcg tcttcgtctg gctcgggact gtaacgccga gcgtcggcgc gctgaccgtg
      781 gccatcgtcg ccgagaacct cgcaggcggc atcggcaccg tgatcttcgt cgcctatctc
      841 tccgcgctct gctcgagccc ggcgcatacg gcgacgcact acgcgctgct gacggcactc
      901 gcctcgaccg ggcgcacgtt catcgcagcc ttctccggct atgccgcgaa gtcgctcggc
      961 tggccaggct tcttcctgat gacggtcgta ctggcgctgc caagcctcgc tgtgctcgtc
     1021 tggctgatcc ggggcgggca ttttgcggcc ctcggcgggg agcgccgcga tggcgcgtga
     1081 ggcggcacca ctccaggcga tcgcgggctt cgtccaggac gaggagggcc attgggtggc
     1141 gctgctggct tgcggtcacc gccagcacat gcgccatgtg ccgcccttca tcgagcggcc
     1201 gtgggtcgca actgagcaag gccgggtcgc caagttcgga acggcgatcc cttgcagtct
     1261 ctgcttggac gacttctcaa gcgtgtacgc tcggtctagt ctcgatgcat cccggaccat
     1321 ggacgaggca agggtgatga cgaaccggct tccactcgac ggcattcgtg tgctcgacgt
     1381 ctcgtcgttc atcgccgcgc cggcagcagc cgtcgtgctc ggcgactatg gcgccgacgt
     1441 catcaaggtg gaggcgccgg gcgagggcga tccgcaccgc accaacatcc agctcgcgag
     1501 cttcccgaag gccgaggtca actatccctg gcatctcgat gcgcgcaaca agcggtccgt
     1561 ggcgctcgac ctcaagtcaa aggacgggcg ggcagcactc gatcggctga tcgagagcgc
     1621 cgacgtgatg atcacgaact ttccgtttcc cgtgcgcgat cggttgacac tcggctacga
     1681 ggccgtggcc aaggtgaacc cgaagctcat atacgcgtcg ttctcagggt acggcgagga
     1741 gggtgacgag aaggaccagc tcggcttcga tgccaacgcc ttcttcggcc gggcgggcat
     1801 cctcgaccag gcacgctacg agggccagcc gccggccttt gcgctgccgg cgcagggaga
     1861 tcgctgctcg gccatgtcgc tcgtcaccgc gatcatgatc gcgctcttcg accgggctcg
     1921 gacggggaag ggcacctggg tcgcaagttc gctctatgcc aatggcctct ggtcgaacgg
     1981 cgtgcacgcg caggcggcgc tcctcggctc ctatctgccg ccgcgtccgc cgcgcgaccg
     2041 gccgaggagc gccgtcgcca atatcttcgt caccaaggat gaccgctgga tgcaggtctc
     2101 cgtcgtgcgc gaggacaaga tgtggtcgac gttcttgacg gcgatcgagc ggccggatct
     2161 tgcaagcgat ccgcgcttcg ccgagaccgc gatccggcgc aagaatgcgg ctctgctgac
     2221 gacgattctc gatcctatct tcaaggggcg cacatgggcg gagtggcacg ccatccttct
     2281 ccgccacaat ctgccgcaca gtccgatcgc acgcgccgag gacatatcgg tcgatccgca
     2341 agcggtggca gcgaaggcga tcgtcccgac ggcaatcgcg gagatgccgc gcaccattgc
     2401 cgcgccgttc caggtcggcg gtcacgagcc gcgcaaggct ggtccaggac ccgggctcgg
     2461 ggcgcacact gacgagatcc tgcgggctgc cggctactcg gtcgcggata tcgcggcgat
     2521 gcgcaagtcg ggcgccgtcg ggtgagcctg gccccgttca gatgtctggc gagcgggcgc
     2581 gctcccatga tagggctacg ccatgcctga caatgagccc tccagcagga ccacgccgaa
     2641 gctcgtcgat gactgcttcc gcatcgacgg gcgccgcctg acgcatggcg aggcgatcgc
     2701 cttgttgaag gagcgggcag ccccgatcgc cggcgagacc tccgtcaccc ttgcagaggc
     2761 ggacggccgc gtgattgccc ggccgatcgt ggcgccgcgc cctatcccga tgcacgcgaa
     2821 ctccgctgtc gatgggtatg cctttgcttg ggccagctac gaccgcgagc gtggttccac
     2881 actgcccgtc gtcgggcgtg ccgcggcggg tcggccgatg cgcgaagccg tacctgcggg
     2941 agcggcggcg cgtatcttca ccggcgccgc aatgccggcg ggcaccgaca cggttgtgat
     3001 gcaggaggac acagcgacgc gcgccgtcga cggtcgggac tgcgtcgtcg tcccccctgg
     3061 cttgaagacc cgcgccaatg cccgagccgc cggagaagac gtcgcgaccg ggcagaaaat
     3121 attcgaaccc ggccatgcca tcaggccgca ggatatcgca gcgctcgcct cgctgggcct
     3181 tgcagtggtg cctgtgttcg atcgtctgaa ggtcgccatc gtcgcctcgg gagacgagat
     3241 cgtccgtccg gacagcaagc gcgccggcgc aatcgaggtc ggccaagtct acgacgcgaa
     3301 cagtccgatg ctggcggcgc tgctgcggag cccgtcgatc gaggtcgacg atctcggcgt
     3361 cctgcctgac gacgcggcaa aggtgcgggc gcggctcgtc gaggcggctg aactctatga
     3421 tgtgatcctc acaagtggcg gcgcgagcct cggcgatgag gaccacatgg tcgcggcgat
     3481 cgatgcgctc ggaacgaggc acatgtggca gctggccatc aagcccggcc gaccgatctc
     3541 gttcgggcag atcgggcgcg ccgtcatcgt cgggttgcca ggaaatccgg tcgccgtgtt
     3601 cgtctgctat ctgatgtacg tctacccgct gcttcgcagg ctcgcgggcg cgccctggcc
     3661 gacgcctcgg cggctaagtc tgccggcgct cttttcattc aagggcagga agaaggggcg
     3721 ccgggagttc tggcgcggca tgctcgtcga tggcccggac ggtctcggcg tcgacaagtt
     3781 catgcgcgac ggctcgggcc tcatctcttc gctgcgcgcg gcggacggcc tcatcgatat
     3841 ctgcgaggat cagggcgacg tggcgccggg cgatttcgtc gacttcattt ccttttcgga
     3901 gttcggcctt gcgggccgtt gacgggcgcg ctcagacgcg gaaggcccag gccgtcatgt
     3961 gggccggata gggcttcggc tgcgccgcat agaggacgcc gccgccgatc gcacaggcga
     4021 ggacgagggc aatcgtcgtc gtcaaacgac gctggcgctg gcgcgcaaca agatgccccg
     4081 acacgatgtc gcaatccgcg cgcacggtcg cgccgatcgg atcttgtggc tggaatagct
     4141 cgctcatggt cgcagcgctc cttcgcctgc gagcatgggc atgcgagcct gaccgcgagt
     4201 taacgcagca ctccgatcgc cgcgtacggt tagtgaagtc ttactttggc cgagtgggca
     4261 gcactcgcga cgcacattct ttccgccgac gtcgggttac ctttaccgca gctttacatg
     4321 ctccggacta atatcgggaa aaccaatgct tggcgccccg atgcgccctc atcacctgaa
     4381 ccgggcgggc gaaatgacgt tcgatcgact gaaatcgcgc aacgatcagc tgatgtcgag
     4441 gctggcgaaa ataaagggaa gtacagccct caccacggcc cggcgctcac tcgcgctcgg
     4501 cgcacccccg aaggacgcag taacattgga ggcgccggtt cccgagagcc ggacggcctc
     4561 gccggcagcg gagccagagc ccgagatggc gcagaagcgg cgtacgccac gtcgccgcgg
     4621 cgagatcccc tgcacgatcc aattcgacgg gctccgcatg acactgtctg cccgcatcat
     4681 cgatatgtcg gctggcggcg cgcgcctcca tctgccgccc gcggccaaga ccgcctacgg
     4741 cggcgtgcgc gggctgccgg atcggctgac gctgcagatc aaatccgatc gcatggagta
     4801 cgagggcgcg attgcctggc gctcagagag cgagattggt gttcgcttcg tcgcaccgcc
     4861 tcggcagatg aaggtgcagg cgacggcgcc cacgacctct cccgtccgct ccgctcgctg
     4921 agcttcagtc cgggagaagg gcgacgcagg cgaacgcgac gcgcgcctcg ctcggcacgc
     4981 cgaacagcgc ggtatggcgc gcgggcagcc ccttcgggaa atgccggaca tattcctcgt
     5041 tgcaggcctg atagtcctcg cgccgactga tcagcatcgt catttgcacc accagatcga
     5101 gcgagctgcc cgcctcgccg aggatgcggg cgacctcgga aagtgcgttg cgcacctctt
     5161 cctgcggggt cgccccgcgc aggacgccgc gcgccggatc gtgcggcgcc gagtgaccag
     5221 acacccagac catgccgttg gctttggccg cccgcgagaa cggccgcggc tcgcctgttg
     5281 ccggcggctc gatgaaatgg atcatgctca cctcccctgt tcgcagccgt ctccggcgat
     5341 ccttcccgtt atcctgctgc ctagcacgga ctattcacca ggacgtggtc gcgacagagg
     5401 cgaaatttac gaatggccat gtgcgccgcg cagtccgttg caggaacatc ggtcaagcgg
     5461 cgagatattc tggaggcttg cttcatgcaa gacgccattc gccaattgcg cccgccctcc
     5521 gggtcgctca gggcgggtcg cccggtgcgt cggatgtctc gagcgtaggc taaagcgacg
     5581 cttttcgcca tccacctgcc gtccgtgtcc tgcagattgc atccagcgca tggcccatgg
     5641 tcgccgtgcg ccgaatcccg cgcttcgaac ttgctcggga cctaaactgc gatgtgctcc
     5701 tgggagcggg cccggcacat gcagcgcagc gctcctctgc cccatgcaga tcacgcgatc
     5761 gccaagcatc ttgttcccga cattggctga aaataggcaa tcctacaaat tgtcgccgaa
     5821 gcgctggtta ccccatgttt tgttcggcga atttcattgt cattaatcga aaccgatttc
     5881 ctcgcatatg catcgactcg acggcctgtt tgcgggacat ctgtagttgt ctgaaatagc
     5941 tcatctattt accggaaata aaaccgcagc ccttgattgt accgttgtct gaggacttct
     6001 cgtggatgtt ccggacatcg atcccgaacc ggcgccgaca cggatcgcgc cgcgcccgct
     6061 ttagtaaagg tcagacatgt ctctccccgc gaatgccggc caaacccccg attttaattc
     6121 gtcgacggtt gagctccgca gcgtcacgcg cacagttcac tccatccagg cgcattcgcc
     6181 caacgatgtc attgcccacg cgcgagtgtc cggtgccgag tttgccgtca cggacaaggg
     6241 ggtgctcgcc ctacgagatg cgcggggtga actcaccatt ctcgattgga ccgcgccgcc
     6301 agcgggatcg aacgcggaaa tccatgccgc aatcgcagtg cgcgtggcgg aaaggctcaa
     6361 tcgcacgatc gccgccgatt ggatgtcgcg cccagccacg ggtgatggcg gtcatgacgc
     6421 agaaacgtcc gatgctggcg gcaaggcccc caccccgccg cccggattgg agacaccgtt
     6481 tcagacccgg cacagcacgc tcgaaggact tgccgacgag acgcgccgtt ggggccgtga
     6541 cgacatcaag cgggcggaag ccgggtcggc cggcgccgtg ctcagcacgc ccgtgggggc
     6601 cggtcttggt catctcgttg gtctcggcga cgaggatccg ggtcgtcgca acggtgaaaa
     6661 gcagtcgaat gaagacggtg cgcgatttct cggcgaatca gttggtgtcg gctcttcgat
     6721 cgagcatctg tggctgctag gcgatttcga ctatgtgcgc tctgcgctcg agctttcgga
     6781 aaacgccgat tttctcgaac cgccggcatc gccgaaccgg cctcagtatg gcccgctcac
     6841 cgggccaaat cccccgagcc tgatgatcga agacacatca ttttctggcc ggctgtttga
     6901 tccggcgatg gccggagtcc ccgtggtcga tcgccagatc accgtggatc ctcgtttcgg
     6961 caacgcaatc ttcaatatcg acggaacatt cgagttcacg ccggcgaacg gctattccgg
     7021 acctgtcacg ctcgagtttt cgttgaccga cccgcgcacg gggcggatcg agaccgggat
     7081 catcgagatc atggtccagg ccgtcatcga tgcgccgacg atcacgtcag gcacgacgac
     7141 gctcgaggat acgaccgtcg cgacgccggt cagcatcatc ctcaatgatc ccgatggctc
     7201 cgaggtgatt gaccaggtca tcatcaccgg agtgccgacc ggggcggtgc tcagctggga
     7261 tgtgcggctg ccggggctcg tttcggcgaa cccggacggc agcctcaccc tgaccggcac
     7321 gacgaccgag atccaggcgc tgctcgcgtc tctgacgctg ctgccgccgg acgacttttc
     7381 tggccagatc acgctcaatg ccgcggtcgt cgtaaccgaa cgcaatctcg atcctgccat
     7441 tccggggtat ctctcgagca tcacgatcaa tcatccgatc gtaatcgacg tcgtggcgga
     7501 cgccgacatc cccgacgtca ccggcgacga gcggacgacg gacgaggaca cacccgttca
     7561 gctgccgagc cttgctgccg cgctcaacga cctcgacggc tccgaggtac tctccatcga
     7621 ggtccgcaat gtgccggttg gcgcgacgtt caatgttggc cagcccgacc ctgtgcgccc
     7681 cggcgtgtgg ctcttcacgc ttgccgaaat ccaggccggg ccgatcttca tgccgccggc
     7741 caatttcatc ggcgacatca ccatgcagat cgcggcgacc gcgaccgagc aggcgaacgg
     7801 tgaccaggag atcgtccttg atcccatcat cgtgcacgta ctgccggtcg acggtcccgg
     7861 cgtcaccgtg acacccgaca tcaccaatga ggacacgccc gtcctcatcg gtgacaacat
     7921 caccatcctc atcgacgatc cgctcggctc cgagacgctg acgagcgtgg aaatccgcgg
     7981 ctttccgatc ggctcggtga tcacctacac agataccgcc ggaatccctc agaccgtcgc
     8041 catcacggtg gccggcacag gcattctcat cgctggcggt accgaggccc agatccgcac
     8101 agccgtcgcc accgtcagcg tgacgccgcc gcagcatagc gacggagacc tcagcatcga
     8161 cgtcttcgcg acgacaaccg atatccgcgg cctcaccttc accgaccagg cgacgctacc
     8221 cgtcacggtc cgcgccatca tcgacgcgcc gaccatcacc tctggtgcga ccgttctcga
     8281 ggacaccacc gtcggcattc ccctgacggt cgcgctcaca gaccaggacg gttcggagat
     8341 catcaacgcc gtcaccatct ccggtgtgcc cacaggcgcc gtcttcggct ggagcaaaac
     8401 gctgcccggc ctcgtcgccg ccaatcccga cggcagctac accgtcaccg gaacgacggc
     8461 ggagatccag gcgctgctgg ccacgctcac gctgctgccg cccgacgact attcgggccc
     8521 catcacgctg aatgcctcgg ttgtcatcaa cgagacgaac ccggatccgg gcttgccgcc
     8581 aggccttgcg accatgacga tcaatcatcc gatcgtcatc gacgtcgtgg cggacgccga
     8641 catccccgac gtcaccggcg acgagcgcac gacgaatgag gatacgcccg ttcagctgcc
     8701 gggccttacc gcggccctca acgacctcga cgggtcagag gtcttgtcga tcgagatccg
     8761 caacgtcccc gccggcgcga tgttcaacgt cggccagccc gatccggtgc gcatcggcgt
     8821 gtggcttttc acgctcgccg aaatccaggc cgggccgatt ttcacgccgc cggtcaactt
     8881 catcggcgac atcaacatgc agatcgccgc catcgccacc gagcaggcga acagcgacca
     8941 ggagaccgtc ctcgattccg tcattgtcca tgttctgccg atcgacggcc ccgacgtcac
     9001 tgtgacgccc gacagcacca atgaggacgt gcccatactg attggcgaca acatcgccat
     9061 caccattgcc gacccactcg gctccgagac gctgacgagc gtggaagtcc gcggcttccc
     9121 ggtcggctca gtgatctcct ttaccgatac ggcgggcaac ccccagaccg tcaccatcac
     9181 agtcgccggc accggcgtca caattgccgg cggcaccgag gctcagatcc gcgacgccgt
     9241 cgccactgtc agcgtgaagc cgccgcccca cagcgacggc gacttcgccc tcgacgtctt
     9301 cgcgacgacc actgacatcc gcggtctcac cttcaccgac caggcgacac tgccggtcag
     9361 ggtgagcgcg gttgccgacg cgccgacggt atctggcgca ggaaccggcg acgaggacac
     9421 ctttatcgcc gtcccgatta ccgtcacgct ggtcgacgcc gatggctcgg aggtgatcga
     9481 ccacgtcgac atctccaaca tcccggcggg cgccaccgtg cagtggacct ccgctgcggg
     9541 acttgtgacg acgctgccgg acggtacgct gcgcatcacc ggcagcacgg ccgaaatcca
     9601 ggcacggctt gcctcgctcg agatccgtcc ccccgtggac agcgacctga atttcgatct
     9661 tgccgtgctt gccgaggtac gcgaggcgaa cccaagcgag gccggcgacg tggccgtgcc
     9721 gattgcgacc cgcgcgttcg cggcgccggt cgttgtcaac ccggtcgcgg atgcgcccgt
     9781 gatcaccggc acgagctcgg tcaacgagga cggcaccgcc aatcccgccg atccagtaac
     9841 cattgtgccc gtcgagttcg gtcgcaacat tgcgatcacg gcgaccgaca gcagtgatgg
     9901 ctcggagcgc atgacggcga tccgcatcgc aggcctcccg atcggcgcag tcgtcagcta
     9961 cgtgccggtg ggcggcggcg cgctccagac cttcacagtc gctgccggca tcacgacgct
    10021 cgatctcaac ggcggcacag aggctgaaat tcgcgcagcg ctcgcaagcc tggccttgat
    10081 ccctcccccg cacagcgacc aggacatttc gctcgccata acggtcacga agtcggatgc
    10141 gaccgcaatc gacaccgagc ctgccgacgt cgtgcaattc gtcggcacgc acgtcatcgc
    10201 ggtcgccgcg gttgccgatc agccgacgct gacgacgaac ggcatcgggg ccggcggcat
    10261 cggcttcggg ctcgaggatg cgaatgtcgc cttgagcctc acggccgggc atccggacag
    10321 cgatgggacc gagcggctgc gcgaggtatt gatccgcgat gtccctgcgg gattcatcct
    10381 gaccgaaagc tcgccgggcg ccggcatact ggccctcgac gcgggtcgaa acgcctgggt
    10441 cgtgacaggt ccgacggacg ctgccgtcaa tgacgtcctc gccaacctca cgcttgcgct
    10501 cagccccgcg gtcggcgggc cgcgtcagca tctcgacacg gacttcaccc tcaatgtgac
    10561 cgtgacctcc agcgagctcg cgccgacgaa cgacgcgacc ggcgggccgg aagtcgcact
    10621 gctcgaccgc tcggcgacgt ttgccgtcaa catcccggtg cgcgcggttg ccgacggcgt
    10681 cacgccatcg ggcagctcga cgctggtcga ggatcgcgcc gccacgatcg gtcccgactt
    10741 gacttgggcc cacattgatg cggacgggac cgaagccgtc acgcggatcg aggtgagcgg
    10801 atttccggtg ggagcgaccg tcgcctggac gccggcggtc ggcggacctc aactcgtcgt
    10861 ggtgccgaca ggtggcacga cgatcgtcct caccggtgcc gacatccgat cagcgctcga
    10921 cacgctcgcc gtgacggcgc ccgccaattc ggacgccaac ttcagcctca ccttcgacat
    10981 cacgaccacg gacaacgacg gctcgacgat cgtcgatact cacttgcact cggtcattgt
    11041 gcaggcggtg gcggacgccc cgagtgcgct cgtcgctgac ctgtcgacca gtgaggatac
    11101 gccggcgacc ctgtcgatca atgtcggtcg cagcgcggat gatgatgctg ccgacaacag
    11161 cgagacgctc tccgttcgca tcactgtgcc ctcggacgtc ggcggcccgg ttggcgtgct
    11221 gaccggtacg gtacctggcg gcgtgacgtt cacgagtctc ggcggaggcg tctacaccgt
    11281 gaccgcggtc ggcgcggacc ccgcgacgcg cgaggccctg ctcgacagct tcctcgtcgg
    11341 cggcatctcg ttcgagccgc gcgctcaatg gtcgggtgtg ctctctggta cgtccggaat
    11401 ccgtgtcgac gtgacctcga ctgaagcggc ggtcggcggg gagcttgccc cgctcggcgc
    11461 tgagggtgca ccgaacggta ccgacggccc gacgaagacg gagaccgtca cgcgcttcat
    11521 cgggatcgat gtccagccgg tcgtcgacgc gccgacactt gccaacgcca gcactatcat
    11581 tcgtgagaac aacaactcct ccgcgatcac ggatcccgat ctggtgctgg cgctcggcag
    11641 ccgtctcggg ctcgcgacgc tcgataccga cggctcgcaa ggcctctcac tgacgctcac
    11701 cggcttgccg accaatctgc aggccgcgag ctttgcagcg accttccccg acgtcacgac
    11761 atcaatcaac ctcgccacgg gaacgatcat cgtttcgagc acgagtgcgg tcagggcgct
    11821 cgcggtgatc ggctcgctgt ccatcacgct cgctgacgac gatgaccgca acttcacggt
    11881 cgccatcaac ggcacgatga gcgacacgaa cggtgtcgcg agtgccagct ctccgttctc
    11941 cctcacgcat gacgtgatcg tgcgggccgt tgccgatacg ccgactgtca cggtcggagc
    12001 cgcgacgaag ccggcggtgc tggaggattc gggtttcgtc ggctatcccg tgacggttgc
    12061 cctcaacgac acggacggct ccgagacatt ccagtccgtg cggatcgatc tctcgacgcc
    12121 tggtggcggt acgcgtcctg tcgcgcagtt cgccgtgacg gccggcgtca ccttcgattc
    12181 ctcggtcccc gggcgcaccg ttctcacagg ctcggccgcc gacattgcca atgccatggc
    12241 ctcgctcgag gtccgtccgg gcgccgacaa cggcgaggac atcacggtga cggtgacggc
    12301 gatcgccgcg gagagcaacc cagcggaaga caacaacggc gccacggccg gcaacggcgg
    12361 cggcatcgcc ggacccgaga tctcggtgcc cacggcccag acggtccgga cgttcgtcat
    12421 cccggtgacg ccggtcgtcg aggcgccgac gctgaatgcg ccgccgaccg ccaccggcct
    12481 cgaggacatg cgcaacgcgc tcgccggcat ctcgctctcc gggatcgcgg atcccgatgg
    12541 ctcggaggcc cgcttcgtcg agatcgatac gacgagcttc cccgccgggt cgaccttcac
    12601 gtcgggcggc gcgcccgtcg gctcgatcgt cagcggctgg ctccggatcc cggaggcctc
    12661 gctcgctgca ctcgaggtgc agccgccgct gcattttacc ggcaccattg ctctctcgat
    12721 ccgcgcgacg accgttgata cgaccggcac tgacgtcgtc acgacgtcgc tcgcgccgca
    12781 aacgctgacg gtcacgatca atccagttgc ggaccccgcc attgtccctg ccgactcgct
    12841 cggcttcgag gacgccgtgg tggcattcgg tgccgatctt gccaatgtcc ccacggggat
    12901 tcgcgtgagt gacacagtcg tcggctcgcc aacgaatggc ggcgcggaga cgatctcggc
    12961 ggttgcgttg cgcgtgcccg cggacacgag cgcactcacc tacacgctct cgggaacctt
    13021 tgtgccgtcg agtgcgggca catttgccgg ttctggaacc gctgaagtga acttcaacgc
    13081 cgccacgcgg acctatacaa tcacctcgac gatcatcacc ggtgccgcgg acccggcgac
    13141 cctcaccgac gcagagcgct cacaggccga ggccgacatc cgtgccacgc tcgcctcgtt
    13201 ccaggtggcg atgggccaag accgtgacgg caacctcgtc gatgacggcc agcaggatcg
    13261 caacggcctc atcgatgtca ccgtgacgac gctcgacgtg aagaatggcg ttgccgacac
    13321 tgagacaacc tcgttcggcc acgacgtcgt catcctggca cgcgccgatg ctccggcaat
    13381 aacggcgggc ttcactgcca gcggcaacga aagtggcgcg agcaccagca tcattccgat
    13441 cgtcggcgcc agcggcgagc gtgtcgtggc cgatcggagc atcgacaccg acggtgtcgg
    13501 cgaaaattcc ggttggggct cggaagtgct ctcggttcgg atcagtgggc tgccgctcgg
    13561 cgcgagcatc gtcccggtca caggctacac tctgccagcg ggcgcgagcc tcgtcccttc
    13621 cgggtcaaat ttcgtcgtca acgcggccaa cgaggacgat ctcaacgacg tcctcgccaa
    13681 catcgggctc gtctcgccgt actactcggg gcccgcgaca ctcacgttca ccgcgatcac
    13741 gactgagcaa ggccaggccg gagagccagt tggcggcatc gacattgccc aggcgacggc
    13801 cgtggcgact gtcggcatca ccatcctgcc gaccgtggac acgccgatcg tgaaggccaa
    13861 cgcgagcggc ctcgaggata cgatcatcgc catcccgaat gccgtgacgc tcggcgatcc
    13921 tgacggctct gagagctacg tcttccgcat cctcgcaacg agtgtcccgg ccggcgccgt
    13981 catctacggc acgggcaacg tggtcattgc cgaggccggc ggatactaca cgctgaccgc
    14041 cgctgaagcg gcaggtctcg cgatccgtcc gccgaccaat ttctcgacga tcaatccggc
    14101 gacaccggac atcgtactcg tcacggaaac gatcgtctcc gatggaccgg cgggaccggt
    14161 gacgttcacg aactcaatcg ccgtcgccgt gcaaggcgtt gccgatcagc cgccggcgat
    14221 gcccgtgacc gtgtcggcga cggaggatga ggcctacgct atcggtgcgg cgatcgaggc
    14281 gacggtcctc gccaacacgg gcggaccgct cccgagcgta ctcacggata ccgacggctc
    14341 agaggttctg tcgttcgttc tggcggggct tccgccgggc gtgatcccgc aggcgagcgc
    14401 gggcacgatt tcctatctcg ggggcggtcg ctggaacgtc tcggcggacg cagtgccgac
    14461 gctcacgctg ccgcctgtct ccgactactc cggcaacaat ccctatccgg gcctcaccct
    14521 gacggcggct tcgcaggaac tcgacagcga ccaggcgacg tccgccgtgt ggccgctgac
    14581 gataaatgtc gcgccggtga tcgcggcggc caccatcgac gggttctcga gttggtcgcc
    14641 ggaccagacg ctgacggaga cggcagtcga gacagccggc gtctctctcg ccagcgcttc
    14701 aatccacgac tttgccgacc tcgacggctc ggaacgcgtg ctgcgctacg tcttcaatct
    14761 ttcgaccctc atcgccgatg ccgagatcgc gggccgcttg gcggagctgc cgggcgcggg
    14821 atcaggtctc gaaaagctcg tcgccaacta catctcgggc acattcgcct actatgcggc
    14881 accgaccgtc ctgacggtca atggtcagcc cttggctgtc gccgcgggca gcatcgtggt
    14941 gctgccggct gacctcgcct cgatcgctct ccaccccgac ctcttcctcg acagcaatgt
    15001 tggtttctcg atccccgtca gcgcgctgct cgaggacacc gcgggtggtc cgcaatatct
    15061 gatgtcgaca ggccttgacg tcacgatcga cggcatcgcc gacgtgccga ccgtcttcgc
    15121 ggcgaacccg gataacgacg gcaacgccgt tgacgtcgac accttcactc cgctgcaatt
    15181 catcccgcta tcgttcggcg gtgtcacgac cgacatcgac gacgatcggc ccggcggacc
    15241 ggcgggcgat ggtctcgggc ggacgcagtc cgaggacgtc tactacatcc tcagtcttgt
    15301 cggcacctct ggcggaccgg cgcccttcct cgccctggtg gacagcacga cgggccagcc
    15361 cgcgggcctc aacaacggcg acggcacgtt tctcgtcaga ccgggtgagc ttgccaactt
    15421 ggcactcgtt gcggccgact tctctggtcc gccagtgacg atgaacttca cggtgacgac
    15481 ggtcgcggtt gacgatgcgt cgcttgcgac aagcgcggct ccggcggcct tctcggtcat
    15541 cgtcgatcct ggcctcggag gaacgacagg cacgccaccg ccggtgccca cggtcaatct
    15601 cgccaacctg ctcggcggta ccgaggacat cgtcggtgca ctcgccgatc cgtcgcagcc
    15661 cgtcgtcacc tccgacccga gcgtgcagtc gacggcagtc atgttcacgg ttccggtcgg
    15721 agccactgtc gcgggcgcag cctggaaccc ggtgacgtcg cgatgggtcg cgaccgctgc
    15781 cgatttcaat gctgggctcg tcacggtgca gccgacttct cgggaagcat gaccatcatc
    15841 atcgaggcgg tcgcgacagg caccaatctc atccgcgcca ccaccgggcc gatcgcgact
    15901 tctgtctacg tcgatccagt cgctgacggc ccgtccgtca cggcaagtcc cgatccggca
    15961 ttcgaaggcg cggcgttcga tctcaacatc gcgcttgcgg agcgcgacat cgacggcagc
    16021 gagacgctcg atggctttgc gtacgtccgg ctcaacaacg gcgcggcgct ggttggcgta
    16081 ttcccactcg ttgcgatcga tgacgcagat gcgaccatcg acggccagag cctcgtcggg
    16141 ttctaccgtg taccggctcc atcgctcgcc acgctccagg ctctcggagc gccgggctgg
    16201 catgggtcgg tgaacatcga aatcgccgca gttgcacgtg acaactcctc tccggccgat
    16261 ccgacgccgg acgcggacaa tacgcagctc tcgctgatca cggtcgcgac cgccattgcg
    16321 gcggtcgcca acgcgccgat catcccggcc agcatcccga cggtgagcgg caacgaggat
    16381 gcagccggcg gcatcccgct ctcgggactt tccgccgccc tcgatgatgt cgtgacaacc
    16441 aacggtggcg agcagctctc cgtggtcatc tcgggggcgc cgctcggcac gcggttctcc
    16501 tcgggcacga acaacggtga tgggtcgtgg accattccgg tctccgccct ggcgacgctg
    16561 cggatcatcc cgccgctcga ttactcgggc cggatgagcc tttcgctgaa cgctgtggcg
    16621 ctcgagctgt ccaatggcga tgaggcgacg tcatcggtcg ccttttttgt cgatgtcaca
    16681 ccggtcgcgg acagcgtcga gatactgacg cccaatgtcg ttgtcccggc agcaggcctc
    16741 gcggcggtac ccatgaacgt gcgcatgcag gacgacagcg ggctttcggc tctcgagact
    16801 gcgccggagt tgttgcgcgt gaccttcacc ggcgtgccga ccggcgcctt cttcgtggcg
    16861 agtcacggcg gcacgctcac ggagccgacg acgggaacct ggatcttcac cggcaccgaa
    16921 gcccaggcga acgcgctgca ggttgcaagt ggcccgaacg tcgcggcggg tacgtccacc
    16981 gtcctgatct ccgccgtcac catcgacggg gcgagcacgc ttgcaactcc cgtactcgac
    17041 agcttcgagc tgacattcgc cgccccgagc aacccgggca cgtccgtcgc cggcaccggt
    17101 tcaggcgaga cgctgtcggg cggcgcaggc aacgacgtga tcaccggtct gggcggcggt
    17161 gatacactgt cgggacttgg cggcatcgac cggcttcagg gcggcacggg cgccgacaac
    17221 ctgagcggtg gcagcgggcg cgacctcttc atctggtcaa cgggcgatac cgtcggcggc
    17281 gccgacacga tcagcgactt cacgaacggt gtcgatggag atgccatcga cgtctcggcg
    17341 ctgctcgtcg gcttcaacgc gcagacggcg gtcctatccg aatacttgcg cctgaagccc
    17401 ggcgatgcga cgaccatcca ggtcgatgca gacggcgata tcggcggagc caacttcgtc
    17461 gatctcgcga ccctcaccgg tgcgaccggc ctcgatgtcg acgcgatgcg gcagaacggc
    17521 aatctaatcg tttgacgatg tcaggcgggc tttcgccgat gccttaggtg aggcgtggtg
    17581 ggcccggcag gactcgaacc tgcaaccaag cggttatgag ccgccggctc tgaccattga
    17641 gctacgggcc ctccggcgca cggcatatgc gccctcagcg catagccgac cggtcgcggc
    17701 ttgtcacgcc gcggccgaac ccgccttgat cgaggcgcgg cccggatagc gcgcgacgtc
    17761 gccgagctcc tcctcgatgc ggatgagctg gttgtacttg gcgaggcggt cggagcgcga
    17821 gagcgagccc gtcttgatct gcccgcaatt ggtcgcgacc gcgagatcgg caatcgtcgc
    17881 gtcctccgtc tcccccgatc ggtgcgacat gaccgcggta tagcctgcgc cgtgggcgag
    17941 attgacggcg tcgagcgtct cggtgagcga gccgatctgg ttgaccttga cgaggatcga
    18001 attggcgacg ccgcgcttga tgccctcggc aaggcgctcg ctgttcgtca cgaaaagatc
    18061 gtctccgacg agctggcact tcttgcctac gagctctgtc agcgccttcc agccggtcca
    18121 gtcgtcctcg ctcatgccgt cctcgattga gacgatcggg aagcgggcga cgaggtctgc
    18181 gaggtacttg gccatgccgg caggatcgag cgtcttgccc tcgccttcca tggcgtaccg
    18241 gccagccttg aagaactccg tcgcggcgca gtcgagcgcc aggaccacgt cttggccggg
    18301 cttgtagccg gccttctcga tcgacttcat gatgaagccc aaggcttcgt ctgcgctctt
    18361 gaggttcggc gcgaagccgc cctcgtcgcc gacgttggtc gcgtggccgg cctccgagag
    18421 gcccttcttc aaggtgtgga acacctcggc gcccatgcgg atcgcgtcgc ggcaggtcgc
    18481 ggcgccgacc ggcatgatca tgaattcctg gatgtcgatc gggttgtcgg cgtgcgcgcc
    18541 gccgttgatg atgttcatca tcggcaccgg cagcacgctc gctctggcgc cgccgacata
    18601 gcggtagagc ggcaggccgg aagcctccgc ggctgccttg gcgaccgcca gcgacacgcc
    18661 gaggatggcg ttggcgccga gccggctctt gttcggcgtg ccgtcgagtt cgatcatcgt
    18721 ctggtcgaca tggatctggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 ncgtgcgcgc cggtcgaggc gccggacgga accgccgcgc ggcccatgga gccgtcctcc
    18901 agcacgacat cgacctcgac ggtcggattg ccgcggctgt ccagaatttc gcggccgatg
    18961 atgtcgacga tggcggtcat gggagcttcc ttattgcgtc cgggaaaagt cgcgcctctc
    19021 ttagcggaag ctcgacgaaa ggcaaatgcg aatcggttta aacaagaacc gggcaacgcg
    19081 ccgctcacgg tttgaaaacc tcggtcacga aatttcatcc ggggtggaac cgagggccac
    19141 gccggcgcgt ttgaaatcgg ctattttcag gggtgcgcgg ggcgaaacnn nnnnnnnntg
    19201 atgggagttt cgccatgtca cagttgagcg gtattctgag tttgttcttc atcgcggcgg
    19261 cgtttctcac ctcgtgcgac agccagccgc ctccgcaggg gttctccgcg gtacatctcc
    19321 tgcagatcga agagtagcga tcgaaaagaa ggcccggaaa tccgggcctt ctcagactgc
    19381 tgacaaaccc ctggcattag ctgggggttt ttgatt
//
LOCUS       DAORNH010000043        19272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2188_length_19272_cov_2.443201, whole genome shotgun sequence.
ACCESSION   DAORNH010000043 DAORNH010000000
VERSION     DAORNH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19272
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2188_length_19272_cov_2.443201"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1001
                     /gene="ubiA"
                     /locus_tag="PK264_06550"
                     /pseudo
     CDS             87..1001
                     /gene="ubiA"
                     /locus_tag="PK264_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
     gene            complement(1014..>2157)
                     /gene="der"
                     /locus_tag="PK264_06555"
     CDS             complement(1014..>2157)
                     /gene="der"
                     /locus_tag="PK264_06555"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRD75582.1"
                     /translation="IDARAGVTPDDKAFARIVRTKSKPVVLVANKSEGRVGEAGALEG
                     YELGLGEPVAVSAEHGEGIGDLAGDILDMLGLAAKATARRRRDDAGEADGDSEIDAER
                     DGDIEPTRSRPIRVAIVGRPNAGKSTLVNALLGEERMISGPEPGLTRDSIPSDLTWKG
                     RPIRLFDTAGLRRKARITERSEKLAASDAVRAIRFAEVVVLLLDAERPFEHQDLTIGG
                     LVANEGRAIVVAVNKWDLVADKQKRLTELRAMLASTLAQVAGCRLVTVSALSERGIDK
                     VMDAVLDAYEIWNKRVATPDLNRWLAEAVVRNSPPAVSGRRIRLRYITQPSARPPTFI
                     TFCSKPDDVPQSYIRYLTNSLRDVFGLPGTPIRFHLRKGDNPYDRS"
     assembly_gap    2158..2167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2168..2427)
                     /locus_tag="PK264_06560"
     CDS             complement(<2168..2427)
                     /locus_tag="PK264_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRD75583.1"
                     /translation="MPLSVAIIGRPNVGKSTLFNRLVGRREALVSDIPGLTRDRREGT
                     AEIGGHTITLIDTAGLEDAPEGSIPARMREQTIAAIDRAEAIL"
     gene            complement(2468..3079)
                     /gene="rplI"
                     /locus_tag="PK264_06565"
     CDS             complement(2468..3079)
                     /gene="rplI"
                     /locus_tag="PK264_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRD75584.1"
                     /translation="MKVILLERVGRLGQIGDVVTVKDGFARNFLLPRGKALRATAANQ
                     KDFEGRRSQLEARNLELKKEADGVGDKLSGKTFVVIRQAGDTGQLYGSVSTRDIAEVV
                     TAGGFSIDRGQVQLDRPIKALGLHGVRIQLHPEVQSKVSVNVARSAEEAERQARGEDV
                     TVIKDEKIELETFSEDAMFEEGGPRRRDEGDAAIPGTEDQPRV"
     gene            complement(3135..4163)
                     /locus_tag="PK264_06570"
     CDS             complement(3135..4163)
                     /locus_tag="PK264_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75585.1"
                     /translation="MLAVFAIGIAAGLTSAILFISAASGSLELGSLLLLVVPLPIMLA
                     GLGWGLTPMAVAAIVGGLSVLTAFRERVMAGTVPARLPGGYIQSIRDIPAYEFLLTQA
                     LPAMILAHLAMLARLERPTEGDEARIDWYPLGRIVAVASVLAGAVASWRLLSFGTTVE
                     TITANIKALFGRLFTIRVPGMPSQPLSAADMDALTSKIVAMLPIGLAVMWLLGMLINL
                     WLAGRITLASGRLLRPWPEFSLIAMPNGFAFAFVGAILATWLGGLPGFFAVAFAGVIA
                     AAFAMTGLAIVHRKTTGNSWRSFILAGTYAFLVIGNVTAVVILALVALAEPMAPWNRN
                     RLPPAPPA"
     assembly_gap    4211..4356
                     /estimated_length=146
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4490..5032)
                     /gene="rpsF"
                     /locus_tag="PK264_06575"
     CDS             complement(4490..5032)
                     /gene="rpsF"
                     /locus_tag="PK264_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRD75586.1"
                     /translation="MPLYEHVFLARQDVSQAQVDALIKEFTEVIEQGGGKVVRHEYWG
                     VKSIAYKIKKNRKAHYTMLHLDAPHPAVAEMERRMGLSTDIIRYLTTRVEAHEAEPSV
                     MMRKSDRDDRRGPGDRDGGPRGPRPFRGDRPPRDRDGDGPRGDRPPRDRDGDGFRGPR
                     GDRGDRPPRPPRDAAPRSET"
     gene            5591..5965
                     /locus_tag="PK264_06580"
     CDS             5591..5965
                     /locus_tag="PK264_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75587.1"
                     /translation="MAGALAAVIAVGTAIAAPQPDKSGIDAIAGSAVTKVHQGCHYDN
                     RWSPGFGWHNHSNSDCRPEAPRPRPRPGYGYGYEPKGYGYGPGGHSGHRENECHYDCR
                     YSPIHGWHNHSNAQCRPEPCRR"
     gene            6105..7145
                     /locus_tag="PK264_06585"
     CDS             6105..7145
                     /locus_tag="PK264_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD75588.1"
                     /translation="MSQKLGTLEELPQDYRDAMARAGVGPLWPQMRNALPHNAPQPVT
                     RSHLWSYEAVRPLLLRAGELTPVEKAERRVLFLSDPGRASAMQTTSTIYIGLQLLLPG
                     ETAPAHKHTPSAARIAVEGEGAYTVVDGEKLPMLPGDLILTPSGCWHDHGHGGKGPFV
                     WLDALDLPLFVYLEGSYAVEAPLQTPKNRPDPSEVEYLAAGLTPTRRRDRRAPQFPMM
                     RYPWTRTEAALRKLAEHVEGGPVELDYVNPETGESCLAVMGFTAMMLKPGAPSRPALK
                     SSSAVFHVVSGAGASTINGVRQSWKAKDTFSAPVFAAIEHEAKGGPAFLIRIHDAPLQ
                     EKLGFYEERVRS"
     gene            7193..7663
                     /locus_tag="PK264_06590"
     CDS             7193..7663
                     /locus_tag="PK264_06590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75589.1"
                     /translation="MSEAWSIRALEAPDLPQLLELYPHLNPSDEVPSLEVAGRRLADL
                     RSYRGSEIFAGFVFGRMVSSCTLIVIPNLTRGGLPYGLIENVVTHTAHRGQGYGKELL
                     KVAAAAAWQASCYKVMLMTGSKQPATLAFYEAAGFEQSKTGFQMRRVPRRETLA"
     gene            7725..8366
                     /gene="maiA"
                     /locus_tag="PK264_06595"
     CDS             7725..8366
                     /gene="maiA"
                     /locus_tag="PK264_06595"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HRD75590.1"
                     /translation="MKLHGYFRSSAAYRCRIAFNLKGVTPESTSVHLRKGEQRSAAFL
                     KLNPNGLVPALEEGGRVLTQSLAIIEWLDETHPSPPLLPKDPFDRAHVRALAFSIACD
                     IHPLQNLRVLAYLRTELDQDQAGLDKWCRRWIESGLAAYEATLVATAAAGRFSFGDAP
                     TIADICLVPQVFSAGRFGADISKLATIKRIMANAEAHPAFAAAHPSKQPDFEP"
     gene            8410..9615
                     /locus_tag="PK264_06600"
     CDS             8410..9615
                     /locus_tag="PK264_06600"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HRD75591.1"
                     /translation="MTTDKPIIIAGGGIGGLATAVGLASKGFRSIVLERAAALGEIGA
                     GIQLGPNAFHAFDYLGVGDAARKMAVYIDALRLMDAMTGEDIATVPLDEPFRRRFGNP
                     YAVVHRGDLHGVFLKACQASPLVDLRTSSEVTGYEQSGGTVRAILKGGAKEDGAALVG
                     ADGLYSKVREQLVGDGKPRVTGHTTYRSVIATDEMPEDLRWNAATLWAGPKCHIVHYP
                     LSGWKLFNLVVTYHNNAPEPVAGKPVPREEVMQGFGHVSARARKIIEHGRDWKLWVLC
                     DRDPVLNWVDGRVAILGDAAHPTLQYMAQGACMAMEDAVCLTHEMAAARGDAALAFEA
                     YRKKRVHRTARIQIQSRLIGDHIYHPDGGHAALRNAMMRSMTAEDYYQSLQWLYGGSG
                     LAEGSGAVI"
     gene            9760..10439
                     /gene="aceE"
                     /locus_tag="PK264_06605"
                     /pseudo
     CDS             9760..10439
                     /gene="aceE"
                     /locus_tag="PK264_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946180.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; frameshifted; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
     assembly_gap    10440..10489
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10490..12428
                     /gene="aceE"
                     /locus_tag="PK264_06610"
     CDS             <10490..12428
                     /gene="aceE"
                     /locus_tag="PK264_06610"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HRD75592.1"
                     /translation="EKLDNLIFVINCNLQRLDGPVRGNGKIVQELEGDFRGAGWNVIK
                     VLWGTGWDELLAKDHSGKLRQLMEECVDGEYQDFKSKNGAYVREKFFGRYPETAAMVA
                     DWSADRIWTLTRGGHDPAKVYAAYKAAVEHKGQPTLILPKTVKGYGMGEAGEGQMITH
                     QQKKMGEAALLAFRTRFNIPLSDEEALKLPFLRLPEGSPEMKYLRARRQALGGYLPSR
                     RPKSETLKAPPLSTFDAQLKGSGEGREISTTMAFVRILNTLLRDKVIGKRVVPIVPDE
                     SRTFGMEGMFRQYGIFSQVGQLYRPQDADQMMFYKEDRQGQLLQEGINEAGAMASWIS
                     AATSYSTSNVPMMPFYIYYSMFGFQRVGDLAWLAGDMRGRGFLVGGTSGRTTLNGEGL
                     QHEDGHSHILSATIPNCVSYDPTFAYEVAVIVQDGLRRMLEKQEDVFYYLTVLNENYE
                     HPAMPEGAEAGIVKGMYLLRAASGKSKGKRQRVQLLGSGAILREVIAGAELLEQDFGV
                     DADIWSVTSFTELAREAQEAERWNLLHPLETPRIPYITECISSRGDAPVIASSDYMKL
                     FAEQVRAFVPSRYRVLGTDGFGRSDYRKKLRTHFEVDRHFVVLAALKSLADDNKIPST
                     KIAEAIAKYGINPEKPSPAKA"
     gene            12440..>12867
                     /locus_tag="PK264_06615"
     CDS             12440..>12867
                     /locus_tag="PK264_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRD75593.1"
                     /translation="MAMTDVTVPDIGDFKNIPVIEILVKVGDQVKADDPLVTLESDKA
                     TMEVPAPAAGTVAAINVKVGDKVSEGTLIVQLESGTGASASGKSAQKPAAASAPKTAN
                     LTPVAAAAGDAAPPRARGSATPPPAPSEFGGVHASPSVRRL"
     assembly_gap    12868..13076
                     /estimated_length=209
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13077..13740
                     /locus_tag="PK264_06620"
     CDS             <13077..13740
                     /locus_tag="PK264_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRD75594.1"
                     /translation="KKLSGPHLHRAWLNVPHVTHSDEADITELEDFRKKLDTSGKEKG
                     YRVTMLAFLIKASVAALKAHPTFNASLAPDKESLILKKYFNIGVAVDTPDGLVVPVIK
                     DADRKGILELSQELGLVSKKVRDGKFTPADISGGTFSISSLGGIGGTHFTPIVNAPEV
                     AILGVVRSSMKPVWDGKAFQPRLMLPLCLSYDHRIIDGALAARFTRHLAGILEDVRQL
                     VL"
     gene            13789..>14257
                     /locus_tag="PK264_06625"
     CDS             13789..>14257
                     /locus_tag="PK264_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD75595.1"
                     /translation="MSEIEVRVPDIGDFKNVPVIELLVKPGDDVKADDPLVTLESDKA
                     TMEVPSPTAGKVTRIAVKIGDKVSEGSVLLVLAAAGAPAVAGAATPAPTAPAKPASAP
                     AAKPATGKSDLHAEVLVLGAGPGGYTAAFRAADLGKKVVLVEHAPVLGGVCLNV"
     assembly_gap    14258..14291
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14292..14622
                     /locus_tag="PK264_06630"
                     /pseudo
     CDS             14292..14622
                     /locus_tag="PK264_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
     assembly_gap    14623..14659
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14660..>14951
                     /locus_tag="PK264_06635"
     CDS             <14660..>14951
                     /locus_tag="PK264_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRD75596.1"
                     /translation="GGIIGLEMATVYHALGSKVTVVELMDQIIPGADRDIVTPLMKRI
                     EKRYEKIYLKAKVTRVESGKGGLTVYFEGEGAPKSDTFDKVLVAVGRRPNGRL"
     assembly_gap    14952..14971
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14972..15539
                     /locus_tag="PK264_06640"
     CDS             <14972..15539
                     /locus_tag="PK264_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD75597.1"
                     /translation="AVDERGFIAVNKQMRTSAANIFAIGDVVGQPMLAHKAVHEGKVA
                     AEVAAGKKSAFDARVIPSVAYTDPEVAWVGLTETEAKAKGMKYGKGVFPWAASGRSLS
                     LGRDEGVTKVLFDEATERIIGCGIVGPHAGDLIAEAALAIEMGADAHDIGATIHPHPT
                     LSETVGFAAEMFEGTITDLIPPKRKAHG"
     gene            15605..16348
                     /gene="phbB"
                     /locus_tag="PK264_06645"
     CDS             15605..16348
                     /gene="phbB"
                     /locus_tag="PK264_06645"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRD75598.1"
                     /translation="MSNRVALITGGMGGLGETISTKMATAGYRVAVTYSPSNKTSGQW
                     LADMKGKGFDFRAYPCDVASYESCEACVKAVVKDLGPVDILVNNAGITKDMTFKKMTK
                     ADWDAVIHTNLDSAFNMAKQVHDGMMDRGWGRIINVSSVNGSKGAFGQTNYAAAKAGM
                     HGFTKSLSLESARKGVTVNTISPGYIGTKMVTAIPANVLEGKILPQIPVGRLGKPDEI
                     AGLIIYLCSEEAAFVTGANIAINGGQHMQ"
     gene            16362..16847
                     /locus_tag="PK264_06650"
     CDS             16362..16847
                     /locus_tag="PK264_06650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD75599.1"
                     /translation="MSTAKSWTFGNAPAEEIARLTGRGLLEAIISGRLPQPPICETLG
                     FALVEVGEGTAAFEGEPSARLLNPLGAVHGGWLLTLVDSAGGCAAHSTIPPGVGFGTI
                     ETKANFSRPIRPDSGLVRAEGRVVGRGRQIISTDVRVTDAAGRLLGHGTSTLMVLAPR
                     A"
     gene            16878..18710
                     /locus_tag="PK264_06655"
     CDS             16878..18710
                     /locus_tag="PK264_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HRD75600.1"
                     /translation="MTRSDAARVFGPSAVETVRRADGAILMRQPLALGAYPRAMTDRL
                     DHWAAVAPERIFLAERDAAGGWQTITYAGARALAMGIGEALIGRGLSEDRPLAILSGN
                     GLEHAVLALASMYAGVPHVPISPAYSLVSTDHAKLRAIIDLVTPGLVFAIDGSMFAKA
                     IAAAIPRDTELVVAIRPHDDRPATLLSDLWQSQPGPLIEAAHAAVGPDTIVKLLFTSG
                     STGTPKGVINTQRMLTSNQVMIATALPSLAATPPVIVDWLPWSHTFGANHNLGLVLMH
                     GGTLHIDQGRPLPGAIEATVANLREIAPTAYFNVPRGYEMLLPYFESDDALRRNFFSR
                     LQFIFYAAASLPPHVWRAYEAMAHATTGRRIPFVTSLGSTETAPSATCATEIARDPGV
                     IGIPNVGAELKLVPSAGKLEARLKGPFITPGYWRQPKLTSEAFDEEGFYRLGDALRFA
                     DPADPAKGLVFDGRIAEDFKLSTGTFVSVGPLRARLISHLAPLIRDVVIAGHDRHALA
                     ALLVPEPDTCRAIATDAGEAGVFASQRLHQALAGRLAAFNTTAGGSSERIERALMLAA
                     PLSIDNGELTDKGSINQRAVLARHAALVDELYAAKPSARTIVAL"
     gene            18816..>19017
                     /locus_tag="PK264_06660"
     CDS             18816..>19017
                     /locus_tag="PK264_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HRD75601.1"
                     /translation="MPASALMNVMVAAVRKAGRSLARDFGEVAQLQVSVKGPGNFVSS
                     ADMKAEDILFKELSKVRQDYGFL"
     assembly_gap    19018..19053
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19054..>19272
                     /locus_tag="PK264_06665"
     CDS             <19054..>19272
                     /locus_tag="PK264_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD75602.1"
                     /translation="HRWIVDPLDGTTNFLHAIPIFAISVALERDGQLVAGVIYNPIMD
                     ELYTAEKGKGAFLNDKRIRVAARRSLSD"
BASE COUNT         3239 a         6386 c         6086 g         3019 t
ORIGIN      
        1 cctttgcacc tatctgcagg cgcacggcct tgcggtgatc gagtgcttcg ccggcaggtt
       61 ctccggccgt cccgccggca ggctccgtcg ccgatgcgcc cgcgggcaac tgggtcgacg
      121 cctacgcccc cgagatcgtc cgcccctacc tgcgcctcgc ccgcttcgac cggccgatcg
      181 gcgcctggct gctgctcgcg ccctgctggt ggtcgcaggg cctcgccgag atcgcgacgc
      241 gcgtctccgc gccgaacctc tggtttctcg ccctcttcct gatcggcgcc ttcgtcatgc
      301 gcggcgcggg ctgcgtccac aacgacatcg tcgaccggga cttcgacgcg agcgtcgccc
      361 gcacgcgaag ccggcctatt ccctcaggcc aggtgagcgt gccgcaggcg ctcgccttca
      421 tggcgctgct gctgctcgcg ggcctcgcgg tgctgctgca gttcaatcgc tacacggtgc
      481 tgctcggcgc gagttcgctg atccttattg ccgtctaccc gttcgccaag cgctatacga
      541 actggccgca gctggtcctc ggcctcacct tcaagtgggg ggcgctcgtc ggctggaccg
      601 cggtcacagg atcgctcgcg ctggcgccgc tcgttctcta tgccggctgc atcctctgga
      661 cgatcggcta cgacaccatc tacgcgcacc aggatcgcga ggacgacctc tcgctcggcc
      721 tcaaatcgac ggcgctctgg ctcggcaaat cgacccacac gtgggtcgcg ggcttctatg
      781 cgggtgccgt cgtgctgtgg gtcgtcgccg cgtggctctc cggcgccggc ctcatcgtgc
      841 tcgctgggct cgcgatcgtc agcgtgatgc tcgcctggca ggtcgcgacc ctcgacattg
      901 acgatgcgcc gggctgcctc cgccgcttca aggcgaaccg cgacgtcggc ctcgtcttct
      961 acctcgcgat tgccgccgag atcgtcgccc ggtcgctctg aactccgagg cgatcacgac
     1021 cgatcgtaag gattgtcgcc cttgcgcagg tggaagcgga tcggtgtgcc gggaaggccg
     1081 aacacatcgc gcaggctgtt cgtcaggtag cggatgtagg actgcggcac gtcgtcgggc
     1141 ttcgagcaga aggtgatgaa ggtcggcggg cgtgccgagg gctgcgtgat gtagcgaagc
     1201 cggatgcgcc gccccgacac agccggcggc gagttgcgca ccactgcttc cgcaagccag
     1261 cggttgagat cgggagtggc gacgcgcttg ttccagatct cgtaggcatc cagcaccgcg
     1321 tccatcacct tgtcgatgcc ccgctccgag agtgccgaga cggtgacgag gcggcagccg
     1381 gcgacctgcg cgagcgtgct cgccagcatc gcgcgcagct ccgtcagccg cttctgcttg
     1441 tcggcgacga ggtcccactt gttgaccgcg acgacgatgg cgcgcccctc gttcgcgacg
     1501 aggcccccga tggtgaggtc ctggtgctcg aaggggcgct cggcgtcgag cagcagcacg
     1561 acgacctcgg caaagcggat ggcgcgaacc gcgtcactcg ccgcgagctt ctccgatcgc
     1621 tccgtgatgc gcgccttgcg gcgcagtccg gccgtatcga agaggcggat cgggcgcccc
     1681 ttccaggtga gatcgctcgg gatcgaatca cgcgtgaggc ccggttcggg tccgctgatc
     1741 atgcgctcct ctccgagcag cgcattgacg agggtggatt tgcccgcgtt cgggcggccg
     1801 acgatcgcga cgcggatcgg cctcgagcgc gtcggctcga tgtcgccgtc gcgctcggcg
     1861 tcgatttcgc tgtcgccatc ggcctcgccg gcatcgtcgc gccggcggcg ggcggtcgcc
     1921 ttggctgcga ggcccagcat gtcgaggatg tcgcccgcga ggtcgccgat gccctcgccg
     1981 tgctcggcgg acacggcaac cggctcgccg agtccgagct cgtagccttc tagggcgccc
     2041 gcctcgccca cacggccctc gctcttgttg gcgaccagca cgaccggctt cgacttcgtt
     2101 cgcacgatgc gcgcgaacgc cttgtcgtcg ggcgtcacgc cggcccgcgc atcgatcnnn
     2161 nnnnnnnagt atggcctcgg cccggtcgat ggccgcgatc gtctgctcgc gcatgcgggc
     2221 cgggatcgag ccctcgggcg catcctcgag cccggcggtg tcgatcagcg tgatcgtgtg
     2281 cccgccgatc tcagccgtcc cctcgcggcg atcacgcgtt agccccggga tatcggagac
     2341 gagcgcctcc cgccggccga cgagccggtt gaagagcgtc gacttgccga cattcggccg
     2401 gccgatgatg gcaacggaga ggggcatgtc tcatcggccg actggttgag cctgagattg
     2461 ggcgacgtca gacccgcggc tggtcctcgg tgccagggat ggcggcatcg ccctcgtcgc
     2521 ggcgacgggg accaccttcc tcgaacatcg cgtcctcgct aaaggtctcg agctcgatct
     2581 tctcgtcctt aatgacggtc acgtcctcgc cgcgggcctg gcgctcggcc tcctcggcgg
     2641 aacgggcgac gttgaccgag accttcgact gcacctcggg gtgcagctgg atgcgcacgc
     2701 cgtgcaggcc gagggccttg atcggcctgt cgagctgcac ctggccgcga tcgatcgaga
     2761 aaccgccggc ggtgacgacc tcggcgatgt cgcgcgtcga gacggagccg tagagctggc
     2821 ctgtgtcgcc ggcctgtcgg atgacgacga acgtctttcc gctgagctta tcgccgacgc
     2881 cgtcggcctc cttcttcagc tcgaggttgc gcgcctcgag ctgagagcgg cgcccctcga
     2941 aatccttctg gttggccgcg gtcgcgcgca gagccttgcc gcgcggaagg aggaaattgc
     3001 gcgcgaagcc gtccttcacg gtgacgacat cgccgatctg gccgaggcgg ccgacgcgct
     3061 cgaggagaat gactttcatg ctcttgctcc ggtttcttga tcgagattgg acggcagggc
     3121 cgcagtccca cccttcatgc ggggggagcc ggcggcaggc gattgcggtt ccacggcgcc
     3181 attggctcgg cgagcgccac gagcgcgagg atgacgacgg cggtgacgtt gccgatgacg
     3241 agaaaggcgt acgtccccgc gagaatgaac gagcgccacg aattccccgt cgtcttgcgg
     3301 tgcacgatgg caagaccggt catggcgaac gccgcggcga tgacaccggc aaaggcgacg
     3361 gcgaagaagc cgggcagtcc gccgagccag gtggcgagga tcgcgcccac gaaggcaaag
     3421 gcaaatccgt tcggcatggc gatcaggctg aactcgggcc agggccggag cagccgcccg
     3481 gacgcgagcg tgatgcggcc ggcgagccag agattgatca gcatgccgag gagccacatc
     3541 accgcgagtc cgatcggcaa catggcgacg atcttgctcg tgagtgcatc catgtccgcg
     3601 gcagaaagcg gctgcgaggg catgcccggg acgcggatcg tgaagaggcg gccgaacagc
     3661 gccttgatgt tggccgtgat cgtctcgacg gtcgtgccga agctgaggag ccgccacgag
     3721 gcgaccgcac ccgcgagcac cgacgccacc gcgacgatgc ggccgagcgg ataccagtcg
     3781 atgcgagctt catcgccttc ggtcggccgc tcgaggcggg cgagcatcgc gaggtgtgcg
     3841 agaatcatcg ccggcagagc ctgagtcagc aggaactcgt aggccggaat atcgcggatc
     3901 gactgtatat agccacccgg caggcgcgcc ggcaccgtcc ccgccatgac gcgctcgcga
     3961 aacgcggtga gtaccgaaag cccaccgacg atcgcggcga cggccatcgg ggtcaagccc
     4021 cagccgaggc cggcgagcat gatcggcagc ggcacgacga gcagcagcag gctgccgagc
     4081 tcgagcgacc cggatgccgc cgagatgaac aggatggccg aggtcaaccc ggcggcgatc
     4141 ccgatcgcga atactgcaag catgaaagct gtcccgccgg tggcggttag ggtcgcggca
     4201 gcgcgaggct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct cggagatgta acggccgagc
     4381 gtgcgcacgt ccttgtagtc gatcttcggc gcctgatcgc ccgagaacgg gcaggtcttg
     4441 cggcggcgct ggaaggggcg gcgtgcgggc tgggatgaat cggtcatggt caggtctccg
     4501 acctcggagc tgcatcacgg ggcgggcgcg gcggacgatc gcctcgatcg ccgcgagggc
     4561 cacggaaccc atcgccgtcg cggtcacgcg gcgggcgatc accacgcggg ccatcgccgt
     4621 cgcgatcccg gggcggcctg tcgccacgga acggacgcgg gccgcgcgga ccaccgtcac
     4681 ggtcaccggg gccgcggcgg tcgtcgcggt cggacttgcg catcatcacc gacggctcgg
     4741 cctcgtgcgc ctcgacacgg gtggtgaggt agcggatgat atccgtcgag agacccatgc
     4801 ggcgctccat ctcggcaacc gcgggatgcg gcgcatcgag atgcagcatg gtgtagtggg
     4861 ccttgcggtt cttcttgatc ttgtaggcga tggacttcac gccccagtac tcgtgccgga
     4921 cgaccttgcc gccgccctgc tcgatcacct ccgtgaattc cttgatcagc gcatcgacct
     4981 gcgcctggga tacgtcctgg cgtgccagga acacatgctc gtacagcggc atgcatgggc
     5041 ctcatggttg cgaacgcccg gcgggcgccc tgccaaacgt ctgaaagtgc cgctggcatt
     5101 tcaatgccat gcagccgctt ccagcaccag cctcggcgca aagccccttc agagcctcga
     5161 ggccctgagg aaacgctcgg aagagcggag acaccgggcg ctggaaccgc ggcgagaatg
     5221 agcctcgctc ggtgccaacc gtgcacagca cggctgcccg ttcagcctcc agccgaaaca
     5281 ggatgaggcc ttatagctga tccctcgcga gatgcaaggc gatcatcgca tcgcacgata
     5341 cccctcatcg acgtgcctgc cgacggcgct gtctgcggca agagctacac agaggtcggc
     5401 gatacgtgtc gagacttgcg tggccggcgt cgcgccggtc agtcgatccc gaatgttcag
     5461 gatgtgttca tttcgtggca atcatgcttt cctatcgtcg cgccgcccgc gtggcgcgcg
     5521 ggccatggtc ggaccgcgaa caaccagatg gggacacacg atgagaaacg tcagtaggcc
     5581 gacttggatc atggctggcg ccttggccgc agtcatcgcc gtcggcacgg caatcgctgc
     5641 acctcagccc gacaagagcg gcatcgacgc catcgccggc agcgcggtga cgaaagttca
     5701 ccagggctgc cactacgaca atcgatggag ccccggcttc ggctggcaca atcattcgaa
     5761 ctcggattgc cgtcccgagg cgccccgccc acggccccgc cccggctatg gctacggcta
     5821 cgagcccaag ggctacggtt atgggccagg gggccacagt ggccaccgcg aaaacgagtg
     5881 ccactacgac tgccgctata gccccatcca cggctggcac aaccacagca atgcgcagtg
     5941 ccgcccggag ccctgccggc gctgagcact tgttagctct cggtgtcaga tcccgcgggg
     6001 tctgacacct atctacggcg ttctccgcca cttcgcgctt gtccggagcg cgtggatggt
     6061 gctcactcgt tgcaagagaa gacttgcacg acgaggaggc gtccatgtcg cagaagctcg
     6121 gcacgctcga ggagctgccg caggactacc gcgacgccat ggcgcgcgcc ggcgtcgggc
     6181 cgctctggcc gcagatgcgc aatgcgctcc cccacaatgc gccgcagccg gtgacgcgat
     6241 cgcacctttg gagctacgag gctgtgcggc cgctgctgct cagggccggc gaacttactc
     6301 ccgtcgagaa ggccgagcgc cgcgtgctct tcctctccga tccgggccgc gcgagcgcca
     6361 tgcagacgac ctcgaccatc tacatcggcc tccagctgct gctccccggc gagaccgcgc
     6421 ccgcccacaa gcacacgcca agtgcggccc gcatcgccgt cgagggcgag ggcgcctaca
     6481 cggtggtcga cggcgagaag ctccccatgc tgcccggcga cctcatcctg acgccgagcg
     6541 gctgctggca tgaccacggc cacggcggca aggggccgtt cgtgtggctc gacgcgctcg
     6601 accttccgct cttcgtctac ctcgaaggct cgtatgcggt ggaagccccg ctgcagacgc
     6661 cgaagaaccg ccctgatcca tccgaggtcg agtacctggc agccggtctc acaccgacgc
     6721 gccgccgcga ccggcgcgcg ccgcagttcc cgatgatgcg ctacccgtgg acgcgtacgg
     6781 aggcggcgct gcgcaagctc gccgagcatg ttgagggcgg cccggtcgag ctcgactatg
     6841 tcaatcccga gaccggcgag agctgcctcg ccgtcatggg cttcacggct atgatgctga
     6901 agcccggcgc gccatcgcgg ccggcgctca aatcgtcgag cgcggtcttc cacgtggtga
     6961 gcggcgcggg cgcgtcgacg atcaacggcg tcaggcagag ctggaaagcc aaggatacat
     7021 tttcggcgcc cgtcttcgcg gccatcgagc acgaggccaa aggcggaccg gcatttctga
     7081 tccgcatcca cgacgcgccg cttcaggaaa agctcggctt ctacgaggag agggtacggt
     7141 catgagcgcg acacgcatgc acgtcgagca aacatgtaag tgagagttcg ggatgagcga
     7201 ggcatggagc atacgtgccc tcgaggctcc ggatctcccg caattgctcg aactctatcc
     7261 acatctgaac ccgtcggacg aggttccatc cctcgaggta gccgggcgtc gtttggccga
     7321 tctacgcagc tatcgcggca gcgagatttt cgcgggcttc gtcttcggcc gcatggtttc
     7381 gtcatgcaca ctgatcgtca ttcccaacct gacacgcggc gggttgccat acggtctgat
     7441 cgaaaatgtc gtcacacata ccgcccatcg aggtcagggc tacggcaagg aactgctcaa
     7501 ggttgcggca gccgccgctt ggcaagccag ctgctacaag gtgatgctca tgaccggctc
     7561 caagcagcca gcgacactgg cattctatga agcggcaggc ttcgagcaga gcaagacagg
     7621 atttcagatg cgacgcgtcc cgcggcgaga aaccttggca tagccctacg actggagcgc
     7681 aagccgcgta accgaatcaa ggccacaaag atcgagacga tgccatgaag ctccacggct
     7741 acttccgctc atccgccgcc tatcgctgcc ggatcgcctt caatctgaag ggtgtcacgc
     7801 ccgagtcgac gagcgtccac ttgcgcaagg gcgagcagcg cagcgccgcg ttcctgaagc
     7861 tcaatccgaa tgggcttgtc ccggcactcg aggagggcgg gcgcgtcctc acgcaatcgc
     7921 tcgccatcat cgaatggctc gacgagacgc acccgagccc gccgctgctg ccgaaggacc
     7981 cgttcgaccg tgcgcatgtg cgcgcgctgg ccttctcgat cgcctgcgat atccacccgc
     8041 tgcagaacct gcgcgtgctt gcgtacctga gaaccgagct cgaccaggat caggccggcc
     8101 ttgacaagtg gtgccgccgc tggatcgaga gcgggctcgc cgcctacgag gcaacgctcg
     8161 tcgcgacagc agcagccggc aggttcagct tcggcgatgc gccgacgatt gccgacatct
     8221 gcctcgtgcc gcaggttttc tcggccggac gcttcggcgc cgacatctcg aagctggcca
     8281 ccatcaagcg catcatggcc aacgccgagg cgcaccctgc tttcgccgcc gcgcacccgt
     8341 cgaaacagcc cgacttcgag ccctgacagc tcaaagtaaa ggcagcggca agcgcgacgg
     8401 ggagcggata tgacgactga caagccaatc atcatcgcag gcggcggcat cggcggtctc
     8461 gcaacggcag taggactcgc gagcaagggc tttcgctcga tcgtgctcga gcgcgccgcg
     8521 gcgctcggcg agatcggcgc cggcatccag ctcgggccca acgccttcca cgccttcgac
     8581 tatctcggag tcggtgacgc ggcccgcaag atggccgtct acatcgacgc gctacgcctc
     8641 atggacgcga tgacgggcga ggatatcgcg acagtgccac tggacgagcc cttccgccga
     8701 cgattcggca acccttacgc cgtcgtccat cgtggcgatc tccatggcgt cttcctgaag
     8761 gcctgccagg caagcccgct cgtcgacctc aggacctcat cggaggtaac gggctacgag
     8821 cagagcggcg gcaccgtacg cgccatcctc aaaggaggcg ccaaggagga tggcgcggct
     8881 ctcgtcggcg ccgacgggct ctactcgaag gtgcgcgagc agctcgtcgg cgacggcaag
     8941 ccccgcgtca ccggccatac gacctatcgc tccgtgatcg ccaccgacga gatgccggag
     9001 gatctgcgct ggaacgcagc gacgctctgg gccggtccga aatgccacat cgtccattac
     9061 ccgctgtcgg gctggaagct cttcaacctc gtcgtaacct atcacaacaa tgcgcccgag
     9121 ccggtcgccg gcaagccggt gccgcgtgag gaggtgatgc agggcttcgg gcatgtttcc
     9181 gcgcgtgccc gcaagatcat cgagcacggc cgcgactgga agctctgggt gctatgcgat
     9241 cgcgatccgg tgctgaactg ggtcgatggc cgcgtggcga ttctcggcga tgccgcgcac
     9301 ccgacgctcc agtacatggc gcagggtgct tgcatggcaa tggaagatgc cgtctgccta
     9361 acccacgaga tggcggccgc gcgcggcgat gcggccttgg ccttcgaggc ttaccggaag
     9421 aagcgcgtcc accgcaccgc gcgcatccag atccagtcgc gcctgatcgg cgatcatatt
     9481 taccatcccg acggagggca tgcggcgctg cgcaatgcca tgatgcgctc gatgacggcc
     9541 gaggactact atcagagcct gcaatggctc tatggtggct cgggcctcgc cgaaggctcg
     9601 ggcgccgtga tttgaggttg gacgcggtcg gccgcaagcg ccatgaatgc ctcctaagcc
     9661 ggatcccact ctggtcggac gccgccttcc atctccgacg cttccccgcc cacagccgca
     9721 catcggtcga accgccaacg ccagtcatcg aggacgatga tgtcacaaga ccaggacaac
     9781 gcttcgcacg agacacgcga gtggcaggat gccatctcct cggtgatcgc cttcgagggg
     9841 acggggcgcg ccgatgatct cctgaccgcg gccgtcaatg tcgcgcggcg gtcgggcgcc
     9901 aaggtgccgt tcgccgccaa cagcgcctat gtcaacacga tcccgccgga cgcccagccc
     9961 gagcatccgg gcgatcgcgc gctcgagcac cgcatccgct cgatcatccg ctggaacgcg
    10021 atggcgatcg tactgaaggc caataaggtc tcctcggagc tcggcggcca catcgcgagc
    10081 tttcagtcgg cggcgacgct ctatgacacg ggtttcatgc acttctggca cggcgcggac
    10141 gatcccggcc atggcggtga tctcctgttc atccaaggcc actcctcgcc cggcatctac
    10201 gcgcgcgcct tcgtcgaggg ccgcctcagc gaggaccagc tcaagaactt ccgccaggag
    10261 gtcgacggca aggggctttc gtcatacccg cacccttggt tgatgcctga attctggcag
    10321 ttcccgacgg tgtcgatggg gctcgggccg ctcctcggca tctatcaggc gcgcttcctc
    10381 aagtatctcc acaaccgcgg cctcgccgat accgagaagc gcaaggtctg ggtattctgn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gagaagctcg
    10501 acaacctgat cttcgtcatc aactgcaacc tgcagcgcct cgatggtccg gtgcgcggca
    10561 acggcaagat tgtccaggag ctcgagggcg acttccgcgg cgccggctgg aatgtcatca
    10621 aggtgctctg gggcacgggc tgggacgagc tcctcgccaa ggatcattcg ggcaagctcc
    10681 gccagctgat ggaagagtgc gtcgacggcg aataccagga cttcaagtcg aagaacggcg
    10741 cctacgtgcg cgagaaattt ttcggccgct atcccgagac ggccgccatg gttgccgact
    10801 ggtccgccga ccgcatctgg acgctgacgc gcggcggtca tgatccggca aaggtctatg
    10861 ccgcctacaa ggccgccgtc gagcacaagg gtcagccgac gctgatccta ccgaagaccg
    10921 tcaaaggcta cggcatgggc gaggccggcg aaggccagat gattacgcac cagcagaaga
    10981 agatgggtga agccgccctc ctcgccttcc gcacccgctt caatattccg ctctcggacg
    11041 aggaggcgct gaaactgccc ttcctgcggc tccccgaggg ttcgcccgag atgaagtatc
    11101 tccgggcccg cagacaagcg ctcggcggct atctcccctc gcgcagaccg aaatccgaga
    11161 cgctgaaggc gccgccgctc tcaactttcg acgcccagct caagggtagt ggcgaaggcc
    11221 gcgagatctc gacgacgatg gccttcgtgc gcatcctcaa cacgctgctc cgcgacaagg
    11281 tgatcggcaa gcgcgtcgtg ccgatcgtgc cggacgagag ccgcaccttc ggcatggagg
    11341 gcatgttccg ccagtacggc atcttctcgc aggtcggcca gctctaccgg ccgcaggacg
    11401 ccgaccagat gatgttctac aaggaggatc ggcagggcca gctgctccag gaaggcatca
    11461 acgaggcagg cgccatggcg tcctggatct cggcggcgac gtcctactcg acgtcgaacg
    11521 tgccgatgat gccgttctac atctactact cgatgttcgg cttccagcgt gtcggcgacc
    11581 tcgcctggct cgccggcgac atgcgcggac gcggcttcct ggtcggcggc acgtcgggcc
    11641 gcactaccct caacggcgag ggtctgcagc atgaggacgg ccacagccac atcctttcgg
    11701 cgaccatccc gaactgcgtc tcctacgacc cgacatttgc ctacgaggtc gccgtaatcg
    11761 tccaggatgg actccgccgc atgctcgaga aacaggagga cgtgttctac tacctgaccg
    11821 tcctcaacga gaactacgag catcccgcga tgcccgaggg cgccgaggcc ggtatcgtca
    11881 agggcatgta cctccttcgc gcagcgagtg gaaagagcaa aggcaagcgc cagcgcgtgc
    11941 agctgctggg ttcgggcgcg atcctgcgtg aggtgatcgc gggcgctgag ctgctcgagc
    12001 aggacttcgg cgtcgatgcc gacatctgga gcgtgacgag ctttaccgag ctggcgcgcg
    12061 aagcgcagga agccgaacgc tggaacctcc tccaccctct cgagacgccg cgcattccct
    12121 acatcaccga gtgcatctca tcgcgcggcg acgcgcccgt catcgcctcg agcgactaca
    12181 tgaagctatt cgccgagcag gtgcgcgcat ttgtgccgag ccgctaccgc gtgctcggca
    12241 cggacggctt cggccgctcc gactacagga agaagctccg gacgcatttc gaggtcgacc
    12301 gccacttcgt tgtgctcgca gcgctgaagt cgctcgccga cgacaacaag attccgtcga
    12361 ccaagatcgc cgaggcgatc gccaagtacg gtatcaatcc agagaagccg agcccggcga
    12421 aagcctgagg aaaccagcca tggccatgac tgacgtgacg gtccccgaca tcggcgactt
    12481 caagaacatt cccgtcatcg agatcctcgt gaaggtcggc gatcaggtga aggccgacga
    12541 tccgctcgtc acgctcgaga gcgacaaggc gacgatggag gtgccggcgc cggcagcggg
    12601 cacggttgcg gcgatcaatg tcaaggtcgg cgacaaggtc tccgaaggaa ccctgatcgt
    12661 ccagctggag agcggcaccg gcgcgagcgc gagcggcaag tccgcacaga agccagcggc
    12721 agcttccgcg ccgaagaccg cgaacctgac gccggtcgcc gccgcagccg gggacgctgc
    12781 cccgccgcga gccagaggca gtgcgacacc gccgcccgcg ccctccgagt tcggtggcgt
    12841 gcatgcctcg ccgagcgtgc gccggctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaag
    13081 aagctttccg gcccgcatct gcatcgcgcc tggctcaacg tgccccacgt cacgcattcc
    13141 gacgaggcgg acatcaccga gctcgaggac ttcaggaaga agctcgacac atccggcaag
    13201 gagaagggct atcgggtgac gatgctggcc ttcctgatca aggcgagcgt cgcggcactg
    13261 aaggcgcacc ccacgttcaa cgcctcgctc gcgccggaca aggaaagcct gatcctcaag
    13321 aagtacttca atatcggcgt cgcggtcgat acgcccgacg gcctcgtcgt ccccgtgatc
    13381 aaagacgccg accgcaaagg catcctcgag ttgtcgcagg agctcggcct cgtctcgaag
    13441 aaggtgcgcg acggaaagtt cacgccggcc gacatctccg gcggcacgtt ctccatctcg
    13501 tcgctcggcg gcatcggcgg cacccacttc acgccgatcg tcaacgcgcc ggaggtcgcg
    13561 atcctcggcg tcgttcgctc ctccatgaag cccgtgtggg atggcaaggc attccagccg
    13621 cggctgatgc tgccgctctg cctctcctac gaccaccgaa tcatcgacgg ggcgcttgcc
    13681 gcccgcttca cgcgccacct cgccggcatc ctcgaggacg tgcgccagct cgtcctctga
    13741 gagcgctgcc atcgaccagc cggccgtcca ttgatcggag catgacgcat gagtgagatc
    13801 gaggtccggg ttcccgatat cggcgacttc aagaatgtgc cggtgatcga gctcctcgtg
    13861 aagccgggcg atgacgtgaa ggccgacgat ccgctcgtca cgctcgagag cgacaaggcg
    13921 acgatggagg tgccttcgcc cacagccggc aaggtcacgc gcatcgcggt caagattggc
    13981 gacaaggttt ccgagggcag cgtgctgctg gtcctggcgg cggctggcgc tcctgcggtc
    14041 gctggagccg cgacgcctgc tcccacggcg ccagcgaaac cggcgtcggc ccccgcggcg
    14101 aagccggcca cgggcaagtc cgacctccac gccgaagtac tcgtgctcgg tgcgggtccc
    14161 ggtggctaca cggctgcatt tcgcgcggcc gatcttggca aaaaggttgt gcttgtcgag
    14221 catgcgcccg tgctcggcgg cgtctgcctc aatgtcgnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn ncgaaggtga tcgacgaggc gggccagatg ggcgcgcacg ggatcgcctt
    14341 caccgcgccg aagatcgaca tcgccaagct ccgcggctgg aaggacggcg tcgtcaagaa
    14401 gcttgtcgga gggctttcgg gcctcgccaa ggcgcgcaag gtgaccgtgg tgcacggcgt
    14461 cggccgcttc gtctcgggaa gcgaggtcga ggtgctgggc gatggcgggc gcaagctcgt
    14521 ctcgttcgac caggcgatca ttgcggcggg ctccgagccc gtcactctgc cgttcattcc
    14581 gcacggcgat ccgcgcgtca tcgattcgac cggcgcgctc gannnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnc gggggcatta tcggtctcga gatggcgacc gtctaccacg
    14701 cgctcggctc caaagtgacc gtggttgagc tgatggatca gatcatcccg ggcgccgatc
    14761 gtgacattgt cacgccgctc atgaagcgca tcgagaagcg ctacgagaag atctatctga
    14821 aagccaaagt gacgagggtc gagtccggga agggcggact cacggtgtac ttcgagggcg
    14881 agggcgcccc caaatccgac accttcgaca aggtgctcgt cgctgttggc cggcggccga
    14941 acggccggct cnnnnnnnnn nnnnnnnnnn ncgccgtcga cgagcgcgga ttcatcgcgg
    15001 tcaacaagca gatgcgtacg agtgccgcca acattttcgc gatcggtgac gtcgtcggcc
    15061 agcccatgct cgcccacaag gccgtgcacg aaggcaaggt cgcggccgag gttgcggccg
    15121 gcaagaagag cgcattcgat gcgcgcgtca tcccctccgt tgcctacacc gatccggaag
    15181 tcgcctgggt cggcctcacg gagaccgagg ccaaggcgaa gggcatgaag tacgggaagg
    15241 gcgtgttccc ctgggccgcc tccggccgct cgctgtcgct cgggcgcgat gaaggcgtca
    15301 cgaaagtcct cttcgacgaa gcgacggagc gcatcatcgg ctgcgggatc gtcgggccgc
    15361 atgccggcga tctcatcgcg gaagcagcac tcgcgatcga gatgggggcc gacgcccacg
    15421 acatcggagc gacgatccat ccccatccaa cactctcgga aactgtcggc tttgccgctg
    15481 aaatgttcga gggcacgatc accgatctga tcccgcccaa acgaaaggcg catggctgaa
    15541 cgcgcccgcg accaataacg aagacgaagt gtggaagagc acgagcaagc aagggagata
    15601 gcgcatgtca aatcgcgtcg cactgatcac cggtggcatg ggcggcctcg gcgaaacgat
    15661 ctcgaccaag atggcaactg ccggctatcg cgtcgccgtc acatactcgc cgtcgaacaa
    15721 gacgagcggc cagtggctcg ccgacatgaa gggtaagggc ttcgacttcc gcgcctatcc
    15781 ttgcgacgtt gcgagctacg aatcgtgcga ggcctgcgtg aaggcggtgg tgaaggatct
    15841 cggtccggtc gacatcctcg tcaacaatgc cggcatcacg aaggacatga ccttcaagaa
    15901 gatgaccaag gccgactggg acgccgtcat ccacacgaac ctcgatagcg ccttcaacat
    15961 ggcgaaacag gtgcacgacg gcatgatgga ccgcggctgg ggccgcatca tcaacgtctc
    16021 ctcggtcaac ggctcgaagg gcgccttcgg gcagaccaac tacgccgccg ccaaggccgg
    16081 catgcacggc ttcacgaagt cgctgtcgct cgaatcggca cggaagggcg tcaccgtcaa
    16141 cacgatctcg ccaggctaca tcggcaccaa gatggtgacg gcgatcccgg cgaacgtact
    16201 cgagggcaaa atcctcccgc aaatcccggt cggccgcctc ggcaagcccg acgagatcgc
    16261 cggcctcatc atctatctct gctccgagga ggcggcgttc gtcacgggcg ccaacatcgc
    16321 catcaacggc ggccagcaca tgcaatgacg gaggcgacag cttgagcacc gcgaagtcct
    16381 ggacgttcgg caacgcgccg gccgaggaga tcgcgcgcct caccggccgc ggacttctcg
    16441 aggcgatcat ctcggggcgt ctgccgcagc cgccgatctg cgagacgctc ggctttgcgc
    16501 ttgtcgaggt cggcgaaggc acggccgcgt tcgaggggga gccgtcggcg cggctcctca
    16561 acccgctcgg cgcggtgcac ggcggctggc tcttgacgct cgtcgacagc gccggaggct
    16621 gcgcggccca cagcacgatc ccgcccggcg tcggtttcgg cacgatcgag acgaaagcca
    16681 atttctcgcg gcccatccgg cccgatagcg gcctcgtgcg cgccgagggc cgtgtcgtag
    16741 gccgcggccg gcagatcatc tcgacggacg tccgcgtgac cgacgcagcc ggccggctgc
    16801 tcgggcacgg gacgtccacg ctcatggtgc tcgccccgcg cgcttgaccg gcgattgcag
    16861 ctggaggatc tcgctccatg acacgcagtg acgcggcgag agtcttcggt ccgtccgccg
    16921 tcgaaacggt gcgccgcgcg gacggcgcga tcctgatgcg ccagccgctg gcgctcggcg
    16981 cctatccacg cgccatgacc gaccggctcg accactgggc ggccgtcgcg cctgagcgca
    17041 tctttctcgc cgagcgggat gcggcaggcg gatggcaaac gatcacctat gccggcgcac
    17101 gcgcgctggc gatggggatc ggcgaagcat tgatcgggcg cggcctttcg gaggatcgcc
    17161 cgctcgcgat cctctccggg aatggcctcg agcacgcggt cctcgccctc gcctcgatgt
    17221 acgcgggcgt ccctcatgtg ccgatctcac cggcctactc gctcgtctcg accgatcacg
    17281 ccaagctgcg cgccatcatc gatctcgtga cgccgggcct cgtcttcgcg atcgacggat
    17341 caatgttcgc aaaggcgatc gcggcagcca ttccgcgaga caccgagctc gtcgtcgcga
    17401 tccgtccgca cgatgatcgg ccggcaacgc tcctctcgga cctctggcag tctcagccgg
    17461 gtccgctgat cgaggccgcg cacgcggccg tgggaccgga cacgatcgtg aagctcctct
    17521 tcacctcggg ctcgaccggg acgcccaagg gcgtgatcaa tacgcagcgg atgctgacct
    17581 cgaaccaggt gatgatcgcg actgcgctgc cgagcctcgc cgcgacgcca cccgtcatcg
    17641 tcgactggct gccctggagc cacaccttcg gcgccaatca caatctcggc ctcgtgctca
    17701 tgcacggcgg cacgctgcac atcgaccagg gccgccccct tcccggcgcg atcgaggcga
    17761 cggtcgccaa tctccgcgag atcgcgccga ccgcctattt caacgtgccg cgcggctacg
    17821 agatgctgct cccctatttc gagagcgatg acgcgctccg ccgcaatttc ttcagccggc
    17881 tgcagttcat cttctatgcc gcggcctcgt tgccgccgca cgtctggcgc gcctacgagg
    17941 cgatggcgca cgcgacgacc ggccggcgca tcccgttcgt cacctcgctc ggctcgaccg
    18001 agacggcgcc gagcgccacc tgcgcgaccg agatcgcgcg cgatcccggc gtgatcggca
    18061 tcccgaatgt cggcgccgag ctgaagctcg tgcccagcgc cggcaagcta gaagcgcgcc
    18121 tcaagggacc gttcatcacg ccaggctact ggcgccagcc gaagctcacg tccgaggcct
    18181 tcgacgaaga ggggttctac cgcctcggcg atgcgcttcg cttcgccgat ccggcggatc
    18241 ccgcgaaagg cctcgtgttc gacggccgca tcgccgagga cttcaagctg tcgacaggaa
    18301 cgttcgtcag cgtcgggccg ctgcgagcgc ggctcatttc gcatctggcg ccgctcatcc
    18361 gcgacgtcgt gatcgcgggg cacgatcgcc atgcgctcgc ggcactcctc gtgccggagc
    18421 ctgatacctg ccgcgccatc gcgaccgacg ccggtgaggc gggcgtcttc gcgagccaac
    18481 gtctccatca ggctctcgcc gggcggctcg ccgcgttcaa cacgaccgcc ggcggcagtt
    18541 ccgagcgcat cgagcgcgcc ctgatgctgg ccgcaccgct gtcgatcgac aacggcgagc
    18601 tcaccgacaa gggctcgatc aaccagaggg ccgtgctcgc ccgtcacgct gcactggtcg
    18661 atgagctcta cgccgccaag ccctccgcac ggaccattgt cgcgttgtag ctgcgcgggt
    18721 atccacgctc cgtagcgctc gctgtggtgc ttgccagggc gcaacggagc cgctaccccg
    18781 ggcgccactc ttccaaagcc gagcgacagg gtcccatgcc agcctcagcc ctcatgaacg
    18841 tcatggtcgc ggcggtccgc aaggccggcc gtagcctcgc ccgcgatttc ggcgaggtcg
    18901 cgcagctgca ggtctcggtg aaggggcccg ggaacttcgt ctccagcgcc gacatgaagg
    18961 cggaggacat ccttttcaag gagctttcga aggtccgcca ggactacggg ttcctgannn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcatcg ctggatcgtt gatccgctcg
    19081 acggcacgac caacttcctg cacgccatcc cgatttttgc gatctcagtc gccctcgagc
    19141 gcgacggcca gctcgtcgcc ggcgtgatct acaatccgat catggacgag ctttatacag
    19201 ccgagaaggg caagggcgcc ttcctcaacg acaagcgcat ccgagtggcg gcccgccgca
    19261 gcctatcgga cg
//
LOCUS       DAORNH010000044        19182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2204_length_19182_cov_2.718147, whole genome shotgun sequence.
ACCESSION   DAORNH010000044 DAORNH010000000
VERSION     DAORNH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19182
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_19182_cov_2.718147"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK264_06670"
     CDS             <1..246
                     /locus_tag="PK264_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75603.1"
                     /translation="TVANVTLAGYQALGDRIQFYLDEDTSRQGQNFFGKPVLAPAAAP
                     DGSTVFVGLAPVIAAVVAKRLSQHRIRVVMPPTLAAG"
     gene            313..1080
                     /locus_tag="PK264_06675"
     CDS             313..1080
                     /locus_tag="PK264_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HRD75604.1"
                     /translation="MKISIDTDARTLTVLDGGAKRELDYYSKEAFEVLSDVWLKTSWN
                     QKYPYTFTWFGRPIVQHPEDMVRLAEVIYTVKPDVIVETGIAHGGSLVYYASILKAMG
                     RGRVIGVDIEIRPHNRTAMEAHELKPLITMIEGSSIAPEIVASVKRLIKPGETVLLIL
                     DSNHSKAHVAAELEAYSGIVTKGSYIVSTDGIMRDVYDTPRGTPGWKSDNPCAANEEF
                     LARHPEFVLEQPKWRFNESALDKVITGWPDCYLKRIA"
     gene            1077..2333
                     /locus_tag="PK264_06680"
     CDS             1077..2333
                     /locus_tag="PK264_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00180 family glycosyltransferase"
                     /protein_id="HRD75605.1"
                     /translation="MSSMNDIGPMSSQRISAIVKDGYTLVMPTYNRVEDLRRNLLFQD
                     AQGLTARVLVLDSSRPDIKERNKRTINSVGLDIEHLTYDPATHPFDKFADGINRVRTP
                     YASLCADDDLLLMGGLRASLAALAREPAAAVAHGYYFLFGHLQDTTNIDITTMLYASP
                     SIDDDEPHLRLRTLMRSYQALTYGVFRTPVIQELYRSMGNLQSLMLRELISGAIPVLR
                     GKSLRVPELYMARAHATGDDSGRKRWHPLEWFMRDAPGLMAEYAEYRRLLINALADSK
                     PARPAEQHGRLIDAIHAGYLFKHMPPRVQDYVIKEELAGRGIDDYWSDQPLQQALVEE
                     HNSEFKPPEKDAFVLTRRVADYFPAGVIDSATGSVGFWPVVTRSQVRDYRFYGSFLDN
                     SLSSILDIDGPAIRELVNTLDLYVTD"
     gene            complement(2323..3189)
                     /locus_tag="PK264_06685"
     CDS             complement(2323..3189)
                     /locus_tag="PK264_06685"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRD75606.1"
                     /translation="MKALVIGATGSLGPALRDGLRACGSEVIATSRGGGSALTAFDLA
                     ATPDSWQLPAADVAYLLAGITSIDRCEGDPVGTHLVNVERTLALAERLARSGTHIVCV
                     STNLVLAGDRPDALVTDRIAPQCAYAAQKAEVERALLAFGPAAAILRITKVAEGLDRL
                     LAGWAERLSVRQPIEPFADLICAPMPRADVTSALVTLGIRRLSGLHQIGADADVSYAE
                     IARRLAAAMGADAALVRPTTSAQAGVKLQAKPRHTTLDARASLVALGLSTISTDRTIE
                     ALIARVLAGLSR"
     assembly_gap    3291..3308
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3309..3795)
                     /locus_tag="PK264_06690"
     CDS             complement(<3309..3795)
                     /locus_tag="PK264_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75607.1"
                     /translation="MLRRLMFILACITAGAVSFAATSIYLNRQGRVVTVNSWSGFLAD
                     RKPAWALAMSAGRLSPTTNVKTKALADGIEITTEFDRANPKDGTDGAAYHFTPGTEPV
                     LKGKVATVAFEITTKVFDSGTHEFEVMVVQNGLKAVGWQRLAARAGRNIYSIKFEPER
                     EA"
     gene            complement(3839..4657)
                     /locus_tag="PK264_06695"
     CDS             complement(3839..4657)
                     /locus_tag="PK264_06695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRD75608.1"
                     /translation="MDENPAETTKRLEAEIARLTAESASRLAALKRLEVEHHKFGPPD
                     YNEDYLRVYNKQLDFMTDPAFCRAYERGITSGHNIGGGGDIGIRYRIHTCTWAARHAS
                     KLPGAFVECGVNTGIFSLAVMDYIGFDHLDKKFYLFDTYSGIPEEQFNDQERAAGKQR
                     INQQYFDVYEIAKRNFAPYKNAVLVRGRIPDTLAEVDITEVAYLSIDMNIQLPEMAAL
                     RHFWPKLTHGGVVVLDDYAWRQHAYRKGVFDDFARECGVEIMTLPSGQGLILKA"
     gene            complement(4782..6098)
                     /locus_tag="PK264_06700"
     CDS             complement(4782..6098)
                     /locus_tag="PK264_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75609.1"
                     /translation="MIADAAAGDIAAFVELLDKPMSSDGLTQAVLTAAAHGKIEILEA
                     VRERVGPRFLQVREHLALRMASRRGDIAMLKFLLASGSPIGARGGQVMRLAAIHRHDA
                     IVDLCAQYGCALFGDAEIGAPLEKIALHQPGPVVFLHLAKTAGTSLGALLLRMFPASE
                     RLHYRHVPGLGTDAITATFSWRLENVVSAFGALPPERQRRPILIGGHFSARLIPHLSP
                     SRFVISMLRDPIERALSVYHYYFVPHQPEMCRGGIDEFFRREDVHTRFGYLFHDHQVR
                     VLSGKSELDPDLAHPAALPSPVQPADVEAVMEMARSRFVLLGLTEQFDHSVVHLARQM
                     GWPLTAMIDRRHNVTEREAGEVVASDETRALIAGVNRNDLALWQQARSLLQRHKAIDE
                     PGFERDLDIYARLMARHEQGAGYPALLAYEHAERGIPDPRLQYFVE"
     gene            complement(6155..7630)
                     /locus_tag="PK264_06705"
     CDS             complement(6155..7630)
                     /locus_tag="PK264_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75610.1"
                     /translation="MAKAAIRTASEPPDDARMTADSSAGARNDVVAATAGRAEPMVRL
                     RGVSKTYRVYANRSDRVREALDPRRRSRHRQFHALHDINLTFPRGATVGILGVNGSGK
                     STLLQVIAGIIPASTGTVEVNGKLAAMIELGAGLNPDLSARENVGVNGVIMGLTRAQI
                     ESRLPSIEAFADIGDFFDQPLKTYSSGMAARVGFALTIHLNPDILIIDEALAVGDARF
                     QQKCFQRFRDLQAAGKTILYVSHDRFSLPRLCTHGVVLHAGRVVFDGDPKGAADCYNE
                     ILINGRPGGSLSGRSMAGGKGTILDSSVDATDTGSKARSEAIRAADAAEAAILAFMKI
                     RAKGDRAPSNPTYNKNEKRIGIGGAQVIDYMLTANGKINPGQIKSGDRVAIAMRIHFE
                     EDVEAPILGIRVRAKDGVLAYATNSMWMGIDVAPRKSGDVASYRFDLDCHLGGNDWFL
                     DLTVARSATELMDDRSGLLHLRCDPEKPSNGLANLPIRFAQ"
     gene            complement(7630..>8186)
                     /locus_tag="PK264_06710"
     CDS             complement(7630..>8186)
                     /locus_tag="PK264_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75611.1"
                     /translation="SRAMPNSFGLEWLPEYLSPEELRKIFAKRYVWMTSNAIVARRDA
                     IERVGGFIKEQEWHSDWFTYYAVGLRHGVCPIPEGLGMIRENPGGYSDTGMRDFKRQK
                     PVLEAIIAAACREPNADLRKIFRQCPAILSVFGQQLGRVLLSKPQYWDIAVPYWKFMA
                     NRYKTNSGRSWPNLINYSIKKALR"
     assembly_gap    8187..8214
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8215..8573)
                     /locus_tag="PK264_06715"
     CDS             complement(<8215..8573)
                     /locus_tag="PK264_06715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRD75612.1"
                     /translation="MSTVSILIPNYNHAKHLHQSLGGACGQTRPADEILVCDDGSKDN
                     SVEVISEFAAKYPQLRLLRNDPNRGMQYTINRLLHESTGDYIVCAAADDELYPKFLER
                     HMERLAKYPDVGMSVSE"
     gene            complement(8849..10141)
                     /locus_tag="PK264_06720"
     CDS             complement(8849..10141)
                     /locus_tag="PK264_06720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HRD75613.1"
                     /translation="MQIDAQALKTSVLAERDEATRLFDELRAGSLDPVGPGVTRDTFG
                     AGEEFAYHVIEAKARDLGLETRRDHGANMMMTLPGSDRRLPRVMTGSHLDSVPNGGNF
                     DGAAGVIAGLVAVAAMKRLGLTPRRDVTVMAIRAEESVWFQVSYIGSRSAFGTLPDGA
                     LEARRIDTGRTLAEHMAGCGADVDAVRARKASLEPQGIRAWVEVHIEQAPQLIEAGRA
                     VAVGTGVPGNFRYPAIRILGEHAHVGLPRRFRRDAVMAASDFALGLDTIWQREEAAGR
                     PMAFTIGRFHTNPREHQLTKVAGEMAMSLDVRAYDKEHLANLERETLALVEAIEARRG
                     VTFEMGPRATADVAPSDRDILAGLTGAARAQGIATMPLASPASHDTATFATAGVPAAM
                     LFVRNANGSHNPHEAMEIDDFLEAATVLTRWLITEALE"
     gene            complement(10221..10589)
                     /locus_tag="PK264_06725"
     CDS             complement(10221..10589)
                     /locus_tag="PK264_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75614.1"
                     /translation="MRARLLIAAVTAALLAPPLAANENYERWPLLKSTFPSTGGGGFV
                     IKGYDPVITGAKCITTFMAVEPGAQPKVHANVAEFDAVEVAGGVLCRNGRWRAFEGGA
                     TGTTPFRIFFKNGVFRGSPQ"
     gene            complement(10682..11443)
                     /locus_tag="PK264_06730"
     CDS             complement(10682..11443)
                     /locus_tag="PK264_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRD75615.1"
                     /translation="MRTLHALCFTLTLAIASAQGARAEDIKVLTAGAMKSVVLEMVPG
                     FEKATGHKVSVKNDTAGGLAKRIEGGEAFDLAVITPAVIDALIKSGKVQAGTRINLAK
                     VGMGVAVKTGAPVPDIASVDAFKRALTSARAVTYIDPKAGGSSGIYFDRLLERLGIAD
                     PVRAKAKLKAGGYVAEALVTGEADLAVHQISEILPVKGVTLVGPLPAEVQNYTIYSGG
                     LASAATAAAGARALLQLLAGPETLPVLKSKGMERG"
     gene            complement(11526..>12480)
                     /locus_tag="PK264_06735"
     CDS             complement(11526..>12480)
                     /locus_tag="PK264_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HRD75616.1"
                     /translation="LSLGGVRHLTGGSKAEGNVTCDALMRLCNKEAVELAIDGGASVV
                     VAAGKPPIIGGKTETVMRVGCGSAVQGIFAKQWHGLADEVIVVDEHITGVFTEHQAGR
                     MLDVPHSGIRVRGRRSTPGRYFQVAHPGSGWGGTDVKDPLEIIKSIDPKTAWPGLRLL
                     MVSTTGEHWGWYVLDEALKPKPAEPPAGIAKLIERIAENCEPALSSVLFMGGAGGSLR
                     AGVTENPVRLTNSVKQRATRVTCGGAPSYVWPGGGITVMVDVSLLPDRSTGYVPTPAI
                     VFPIEFTMARRDYEAMGGYTGDIITLAEARARAKALVMPGS"
     assembly_gap    12481..12536
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12537..12978)
                     /locus_tag="PK264_06740"
                     /pseudo
     CDS             complement(12537..12978)
                     /locus_tag="PK264_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528451.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
     gene            13144..>14073
                     /locus_tag="PK264_06745"
     CDS             13144..>14073
                     /locus_tag="PK264_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD75617.1"
                     /translation="MAQRVDARTLKQWLGEPGEIALLDIREMGPYSQGHPFLAVPLPY
                     SRLEVEIERLVPRRSARLVVFDLKGEGLAETAAKRAEALGYTAVHVLAGGAEGWKAAG
                     YELFDGVNLPSKTFGEIVEIERHTPRITAAELAAMQTRGDDIQIVDGRTIAEYQRFSI
                     PGGVPCPNGELALRIADIVPSPKTKIVVNCAGRTRSIIGAQTLIDWGVPNDVVALENG
                     TQGWFLAGLKREEGATGRMPALPTGAALERAKARAAALLEERGVPTVDARTVERWIGE
                     ADRTTYVFDVRTPEEFKADGIAGSVWAEGGQLIQ"
     assembly_gap    14074..14116
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14117..14735
                     /locus_tag="PK264_06750"
     CDS             <14117..14735
                     /locus_tag="PK264_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD75618.1"
                     /translation="VDGEGVRAPMVAAWLRQLGHDACVLAGGHTSGLKIARAPAKAET
                     VLPPPHRIEARALAAGLEAGTVAAFDLRASAAYRDAHIPGSCWSIRPRIAADASAAKG
                     KRIVLIADEGRLARAAAIDLAEAGHSDVAILRGGIEEWRAEGLDLARSPGAPADSERI
                     DFMFHTLNRNAGSRADAEAYLAWEVGLVDRLDAQERAVFKLWGKH"
     gene            14735..15925
                     /gene="metC"
                     /locus_tag="PK264_06755"
     CDS             14735..15925
                     /gene="metC"
                     /locus_tag="PK264_06755"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HRD75619.1"
                     /translation="MGKSKKRPQTLRTKLVQGGRPKENQYGRSRTVNTPIYRASTVLY
                     ENMAALRETRKQRTEGERAFSYGTSGTPTTFALEDVVTELEGGHATRLVSSGLLAITL
                     AFQAVLRPGDHVLITDSTYEPVRNFVRTWLTPMGIAHDYFPADARGLEGMIRRNTKMI
                     WTEIPGSHLFEMQDLPAICRMAKQRGILVGVDNTWASGILCQPLKLGADISILAGTKH
                     IAGHADVLIGTVTTTESCWKPVNDMASALGLCISSDDAFLALRGVRTMATRLPVHQRS
                     ALELAGWLKKHPKVKSVFYPALPDDPGHEIWKRDFTGACGLFSIELKADQAGADRFVD
                     GLTLFGIGASWGGFESLVMPSNIPGARTATDWSGRGPILRLHAGLEDPSDLIADLEAA
                     LALV"
     gene            15943..17106
                     /locus_tag="PK264_06760"
     CDS             15943..17106
                     /locus_tag="PK264_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HRD75620.1"
                     /translation="MPASPRPFTLAVPEADIADLFARLARTRLPDQAPDGPWAYGTDV
                     GYLGRLIEHWRTRFDWRHWEGRLNAFPQFKVALDGIDLHYLNVPGKGPAPKPLLLLHG
                     WPGSVFEFVDIIPRLTDPASFGGDAADAFTVIAPSLPGYGLSFVAGQKRFGIPAMADC
                     FARLMTDVLGYRKFAAQGGDWGAFTCSAMGVRHPEKLSGIHLNMLSVSPYLPPPANPT
                     DAERRYQDEIRAWAKEETGYIQIQGTRPQTLAFALTDSPAGLAAWIAEKFHVWTDHAG
                     DFETVVSRDHLLANISLYWFTGAIGSSFWPYYERLHGPWPIPDGKTVDVPLGYAAFPR
                     EIRRPPRSLAEKSYTDIRQWTEMAKGGHFAAMEQPEALARDVQAFFRPLRGTF"
     gene            complement(17116..17982)
                     /locus_tag="PK264_06765"
     CDS             complement(17116..17982)
                     /locus_tag="PK264_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRD75621.1"
                     /translation="MSKATALSRLIAAGPIVVAPGAPDALTARLVERAGFPAVYMTGF
                     GATASRLGMPDIGLMSQTEMTGHARDMVRATSLPVIADADTGYGGPSNIERTIEEYVA
                     AGVAAVHLEDQVAPKRCGQMAGIRLIDAAENARRLRCALAARGTAERLLVIARTDALP
                     ATGLDEAVRRAALYRDAGADLLFVDGVKTRAEVEGIARRVAGPKVVSLVDGTDAALLT
                     TRELQDMGFAVVLYAVAALFTAARAVEAALAAIKVSGSLAGIAGKTMSYAEFTEVVRL
                     SHHQGLDDRYGS"
     gene            complement(18068..18565)
                     /locus_tag="PK264_06770"
     CDS             complement(18068..18565)
                     /locus_tag="PK264_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HRD75622.1"
                     /translation="MIRYTLRCDKRHEFEGWFKSSDAFDRQAKRRLVECPKCGSTKVE
                     RSIMAPSIVKGRKSGRGRPAEPAPTTETAPPELAISPAPAPGEPDARQTRELLRQMRK
                     VRDELLSKSEYVGPKFADEARKIHLKETPARGIHGEASKSDVEALREDGIEVYPVPVL
                     PDDQN"
     gene            18837..>19182
                     /locus_tag="PK264_06775"
     CDS             18837..>19182
                     /locus_tag="PK264_06775"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD75623.1"
                     /translation="MHDTPPKHFAEPSPFVARWLEGVGGSGTVLDVACGGGRHLRLAL
                     ARGHRVTGVDRDISGVADLAAEPRATIIEADLEGGAGLAPHLAGHRFDGVIVANYLWR
                     PILGDIIAAVSSA"
BASE COUNT         3247 a         6406 c         6086 g         3298 t
ORIGIN      
        1 accgtcgcca atgtgacgct ggccggctac caggcgctcg gcgaccgcat ccagttctat
       61 ctcgacgagg ataccagtcg gcagggccag aacttcttcg gcaagccggt gctcgccccc
      121 gcggcggcac ccgacggatc caccgtcttc gtcgggctcg cccccgtcat cgccgcggtt
      181 gtcgccaagc gcctatcgca gcacaggatc agagtcgtga tgccaccaac gctcgcggcc
      241 ggctgaccta gcaggcggtt catgggcgag agcggcccgg cacccgccag accacggaac
      301 gagagaggtc ctatgaaaat ttccatcgac acggatgcgc gcacgctcac cgtccttgac
      361 gggggcgcca agcgtgagct cgactactat tcgaaggagg ccttcgaggt tctctcggac
      421 gtctggctca aaacgagctg gaaccagaag tatccctaca ccttcacttg gtttggccgg
      481 ccgatcgtgc agcatcccga ggacatggtc cgcctcgccg aggtcatcta caccgtgaag
      541 cctgacgtga tcgtcgagac gggtatcgcc cacggcggct cgcttgtcta ttacgcctcg
      601 atcctgaagg ccatgggccg cggccgcgta atcggtgtcg acatcgagat ccgtccgcat
      661 aaccgcaccg ccatggaggc gcacgagctg aagccgctca tcaccatgat cgagggctca
      721 tcgatcgcgc ccgagatcgt cgcctcggtg aagcggctca tcaagccggg cgagacggtg
      781 ctgctgatcc tcgattccaa ccacagcaag gcgcacgtcg ccgccgagct cgaggcttac
      841 agcggcatcg tcacgaaggg ctcctacatc gtctcgaccg atggcatcat gcgcgatgtc
      901 tacgacacgc cgcgggggac gcccggctgg aagtcggaca atccgtgtgc tgccaacgag
      961 gagttcctcg cccgccatcc cgagttcgtg ctcgagcaac cgaagtggcg cttcaatgag
     1021 agcgcgctcg acaaggtcat caccggctgg ccggactgct acctgaagcg gattgcatga
     1081 gctccatgaa cgacatcggc cccatgagtt cgcagcgcat cagcgccatc gtcaaagatg
     1141 ggtacacgct ggtgatgccg acctacaacc gagtcgagga cctgcggcgc aacctgttgt
     1201 tccaggatgc gcagggcctc acggcgcgcg tgttggtgct cgattcgagt cgtcccgaca
     1261 tcaaggaacg caacaagcgc acgatcaatt cggtcggcct cgacatcgag catctgactt
     1321 acgatccggc aacccacccc ttcgacaagt tcgccgacgg catcaatcgc gtccgcacgc
     1381 cctacgcgag cctctgtgcc gacgacgatc tcctcctgat gggcggcctc cgagcgtcgc
     1441 ttgccgctct cgcgcgtgag ccggccgccg ctgtcgctca cggctactac ttcctcttcg
     1501 ggcaccttca ggatacgacg aacatcgaca tcacgacgat gctctatgcc tcgccgagca
     1561 tcgacgacga cgagccgcat ttgcggttgc gcacgctcat gcgctcctat caggcgctga
     1621 cctacggcgt gttccgcacg cccgtcatcc aggaactcta ccgcagcatg ggcaacctgc
     1681 agagcctgat gctgcgcgag ctgatctcag gggcgatccc ggtgctgcgc ggaaaatcgc
     1741 tgcgcgtccc cgagctctac atggcccgcg cgcacgcgac cggcgacgac tccggccgca
     1801 agcgctggca tccgctcgag tggttcatgc gcgatgcgcc gggcctcatg gccgagtacg
     1861 ccgagtatcg ccgcctgctg atcaacgcgc tcgccgacag caagcctgcg cgcccggccg
     1921 agcagcacgg ccggctcatc gacgcgatcc atgccggcta tctattcaag cacatgccgc
     1981 cgcgcgtgca ggactacgtg atcaaggaag agctcgccgg tcgcggcatc gacgactact
     2041 ggtccgacca gccgctgcag caggcgctcg tcgaagagca caacagcgag ttcaagccgc
     2101 cggagaagga cgccttcgtg ctgactcgcc gcgtcgcgga ctattttccg gccggtgtga
     2161 tcgattcagc cacgggaagc gtcggcttct ggccggtcgt cacacgctcg caggtcaggg
     2221 actaccgctt ctacggcagc ttcctcgaca actcgctttc gtcgatcctc gacatagacg
     2281 gcccggcgat ccgcgagctc gtcaacacgc tcgacctcta cgtcaccgac tgaggccggc
     2341 gagcacgcga gcgatcagcg cttcgattgt gcggtcggtc gagatggtcg acaaccctag
     2401 cgcgacgagc gacgcgcgcg cgtccagggt cgtgtggcga ggtttcgctt ggagcttgac
     2461 gccagcctgc gccgaggtcg tggggcggac gagcgcggca tctgcgccca tcgccgcggc
     2521 gaggcgccgg gcgatctcgg catagctcac gtccgcgtcg gcgccgatct gatggaggcc
     2581 actcagcctg cgtatcccga gcgtgacgag cgcggaagtc acgtccgcac gcggcatcgg
     2641 tgcgcagatg agatcggcaa acggctcgat cggctgccgg accgagagac gctccgccca
     2701 gccggcaagg agccgatcga gaccctcggc caccttcgtg atcctcagga tcgcggctgc
     2761 gggcccgaac gcgagtagtg cccgctccac ctcggccttc tgcgcggcgt aggcacactg
     2821 cggtgcgatc cggtccgtta ccagcgcgtc cggccggtca ccggcaagga cgaggttggt
     2881 cgagacgcag acgatgtgtg tgcccgaccg cgccagccgc tcggcaagcg caagcgtcct
     2941 ctcgacattg acgagatggg tgccaacggg atcgccctcg cagcggtcga tcgaggtgat
     3001 gccggcgagg aggtatgcga cgtcggcggc gggcagctgc catgagtccg gggtggctgc
     3061 gagatcgaag gcggtcagcg cgctgccgcc gcccctgctg gtggcgatca cctccgaccc
     3121 acatgcccgc aggccgtcgc ggagtgcggg tccgagactg cccgtcgcac cgatcaccag
     3181 agccttcatt tcggcctagt ccctgtagac acggatttcg tggaggacga gtgagcgacc
     3241 gcgaccggag gcatcgctcc tcacccaaat gctgtcgacc tgctgaccgg nnnnnnnnnn
     3301 nnnnnnnngc gcctcgcgct cgggctcgaa cttgatgctg tagatgttgc ggccggccct
     3361 ggcggcgagc ctctgccagc cgaccgcctt gagcccgttc tggaccacca tgacctcgaa
     3421 ctcatgtgtg ccgctgtcga acaccttggt ggtgatctcg aaggcgaccg tcgcgacctt
     3481 tcccttcagc acgggctcgg ttccgggagt gaaatgatag gcggcgccgt cggtgccgtc
     3541 cttggggttg gccctgtcga actctgtggt aatctctatg ccgtcggcga gtgccttcgt
     3601 cttgacgttg gtcgtgggcg acaaacggcc cgcggacatc gccaatgccc aggcgggctt
     3661 gcgatccgcg agaaatccgc tccatgagtt gacggtgaca acgcgaccct gccggttgag
     3721 atagatcgac gtcgccgcga acgagaccgc tccggcagtg atacaagcga ggatgaacat
     3781 cagccgacgc agcatcggga cccctcgttc cgatcaaaca ctcgtcgcag ccatgtcctc
     3841 atgccttgag gatcaggccc tggccgctcg gcaaggtcat gatctcgacg ccgcactcgc
     3901 gcgcgaagtc gtcgaacacg cccttgcgat aggcatgctg tcgccaggca tagtcgtcga
     3961 gcaccacgac tccgccgtgc gtcagcttcg gccagaagtg gcgaagcgcc gccatctcgg
     4021 gcagctggat gttcatgtcg atcgacagat aggcgacttc ggtgatgtcc acctcggcga
     4081 gcgtatcggg gatgcgcccg cggaccagga ccgcgttctt gtacggcgcg aaattgcgtt
     4141 tcgcgatctc gtagacgtca aagtactgct gattgatgcg ctgtttgccc gccgcccgct
     4201 cttgatcgtt gaactgctcc tcgggaatgc ccgaatatgt atcgaacagg tagaatttct
     4261 tatcgaggtg gtcgaagccg atgtagtcca tgacggcgag tgagaaaatt cccgtgttta
     4321 cgccgcactc gacgaacgcg ccgggcagct tcgaggcgtg gcgcgcggcc cacgtgcacg
     4381 tgtggatgcg atagcggatg ccgatatcac caccgccgcc gatattgtgg ccggaggtga
     4441 tgccgcgctc ataggcccgg cagaaggccg ggtccgtcat aaaatcgagc tgcttgttat
     4501 agaccctgag gtagtcctca ttgtagtcgg gcggaccgaa cttgtggtgc tcgacctcca
     4561 atctcttgag ggcggcaaga cgcgacgcgc tctccgccgt caggcgcgca atctccgctt
     4621 cgagccgctt cgttgtctcg gccggattct catccatgtc ccacctagcc tctcccgccg
     4681 caccgtcacg ctcgtgccac ttgcaccgga ccaaactatg atcagtctac cgaacgaacc
     4741 gcttgacagg catatgcgag cgggctgatc ggtggcacac ttcactccac gaagtattgc
     4801 aaccgcgggt ccggtatacc tctctcggcg tgctcataag caagcaatgc cgggtaaccg
     4861 gcgccttgct catgacgcgc catgagccgg gcgtagatgt ccagatcgcg ctcgaagccg
     4921 ggctcgtcga ttgccttgtg cctctggaga agagaccggg cctgctgcca caaggccaga
     4981 tcatttctgt tgacgcctgc aatcagggcg cgggtctcgt cactcgcaac aacctcgccc
     5041 gcctcgcgct cggtgacgtt gtgtcgtcgg tcgatcatcg ccgtcagggg ccaacccatc
     5101 tgacgagcca aatggacgac cgaatggtcg aactgctccg tgagccccag gaggacgaag
     5161 cgcgatcgcg ccatctccat gacggcttcg acatcggccg gttggactgg cgatggcagc
     5221 gccgctggat gagccagatc cgggtcaagc tccgactttc cggagagaac tcgcacctga
     5281 tgatcgtgga acagatagcc gaaacgcgtg tgaacgtctt cacgacggaa gaactcatcg
     5341 atgccaccgc ggcacatctc cggttgatgc ggcacgaagt agtagtgata gaccgacaac
     5401 gcacgctcga tcgggtcgcg cagcatcgat atcacgaatc gggaaggcga caggtgcggt
     5461 atgaggcgcg cggagaagtg cccgccgatc agtatcggcc ggcgctgtcg ttcaggcggc
     5521 aacgcgccga atgcgctcac gacattctcg agccgccaag aaaatgtcgc cgtaatcgcg
     5581 tcggtaccga gcccagggac gtgtcgatag tgaagacgtt cgctcgctgg aaacatgcgc
     5641 agcaagagtg cgccgagcga cgttccagcc gttttcgcga gatgcaggaa cacgactggt
     5701 ccgggctggt gcaacgcgat cttttcgaga ggcgcaccga tttccgcgtc accgaacaat
     5761 gcgcatccgt attgtgcgca gaggtcgaca atcgcgtcgt gtcgatggat tgctgccagc
     5821 cgcatgacct gaccgccgcg cgcgccaatc gggctacctg aggccaggag aaatttcagc
     5881 atcgcgatgt ctcctcgtcg cgatgccatg cgcagtgcaa gatgctctcg cacctgaagg
     5941 aaccgagggc cgactctttc tctgaccgcc tcgaggatct cgatcttgcc gtgtgcggca
     6001 gccgtgagca cggcctgcgt aaggccatcg ctcgacatcg gcttgtctag cagttcgacg
     6061 aatgccgcaa tgtcgccggc tgcagcgtct gcaatcattg atcgtaccac tgcatgtggc
     6121 aagggttgtc ctcaccgtct cacaaacgga gggcctactg agcgaaacgt atcggcaggt
     6181 tggcgaggcc attcgatggc ttttccggat cgcagcgcaa gtgcagcagt cccgaacgat
     6241 catccatcaa ttcggtcgca gatcgagcaa ccgttaagtc gaggaaccag tcgttgccgc
     6301 cgaggtggca atccagatcg aaccggtagc tcgcgacatc accgcttttt cgcggagcga
     6361 catcgatgcc catccacatt gaattggtcg cgtacgccag aactccgtct tttgcccgca
     6421 cccgaatccc gagaatcgga gcctcgacat cctcttcgaa atggatccgc atcgcgatcg
     6481 ccactctatc ccccgatttg atctggcccg ggttgatctt cccatttgcg gtcagcatgt
     6541 agtcgatcac ctgcgccccg ccgatgccga tgcgcttctc gttcttgtta taagtcgggt
     6601 tactcggggc acgatcgcct ttcgctcgga tcttcatgaa ggccaggatt gccgcctcgg
     6661 ctgcatcggc ggctcggatg gcttccgagc gcgctttcga cccggtgtcg gtcgcgtcga
     6721 ctgacgaatc gaggatggtg cccttaccgc ctgccatcga ccgaccggac aaagacccgc
     6781 cgggccgccc gttgatcagg atttcgttgt agcagtccgc ggcccccttc ggatcgccat
     6841 cgaaaaccac gcgtcccgca tgcagcacga caccgtgggt gcagaggcgc ggcagggaaa
     6901 agcgatcgtg ggagacgtag aggatcgtct tgccggccgc ctggagatcg cggaagcgct
     6961 ggaagcattt ctgctggaag cgcgcatctc cgaccgcgag cgcctcgtcg atgatcagga
     7021 tgtccgggtt caaatggatg gtgagggcaa atccgacccg cgccgccatg ccggaggaat
     7081 aggtcttcag cggctgatca aaaaagtccc cgatgtcagc gaatgcctcg atactcggca
     7141 ggcgagattc gatctgcgcg cgcgtcaacc ccatgatcac gccgttgacg ccgacattct
     7201 cacgggcact gaggtccgga ttgaggccgg cgcctagctc gatcatggcc gccagcttgc
     7261 cgttcacctc gacagtaccg gtggaggccg gaatgatgcc tgcaatgacc tgcagtagcg
     7321 tcgacttgcc cgagccgttg acgcccagga tgccgaccgt tgccccgcga ggaaatgtca
     7381 gattgatgtc gtgaagggca tggaactggc gatggcgcga gcgtcgcctc gggtcgagcg
     7441 cctcgcgcac ccgatccgac cggttggcat agacgcggta ggtttttgaa acgccgcgaa
     7501 gccgcaccat aggctctgct cgcccggcgg ttgcggccac gacgtcattg cgggcccccg
     7561 cgctgctatc cgccgtcatg cgcgcgtcgt ccggcggctc gctcgcagtc ctgatcgcgg
     7621 ctttggccac tagcgcagcg ccttctttat cgaatagttg atgaggttcg gccaactgcg
     7681 acccgagttg gtcttgtagc ggttggccat gaacttccag tacgggacgg cgatgtccca
     7741 atactgcggc ttggagagta gcacccgccc gagctgctga ccgaagacgc tcaggatcgc
     7801 cgggcactgg cggaagatct tgcgcaggtc cgcattcggc tcgcggcacg ccgccgcgat
     7861 gatcgcctcg agaacgggct tctgcctctt gaagtcgcgc attcccgtat ccgagtagcc
     7921 gcccgggttt tcgcggatca ttccaagtcc ctcagggatg gggcaaacgc cgtggcggag
     7981 cccgaccgca tagtaggtaa accaatccga atgccactcc tgctctttga tgaagccccc
     8041 gaccctttcg atcgcgtcgc ggcgggcgac gatggcattg ctcgtcatcc agacgtagcg
     8101 cttggcgaag atcttgcgca gttcctccgg gctcaggtat tctggaagcc actcgaggcc
     8161 gaacgagttg ggcatggcgc gcgactnnnn nnnnnnnnnn nnnnnnnnnn nnnnaactcg
     8221 gagacgctca taccgacgtc tgggtacttg gcgagccgct ccatgtggcg ctcgaggaac
     8281 ttcggataaa gctcgtcgtc cgccgcggcg cagacgatgt agtcgcccgt cgattcgtgc
     8341 agcagccggt tgatcgtata ctgcatcccg cgattggggt cgttgcgcag caggcgcaac
     8401 tgcgggtact tcgccgcgaa ctccgagatg acctcgacgc tgttgtcttt cgagccgtca
     8461 tcgcagacga ggatctcgtc ggccggccgc gtctggccgc acgcgccgcc gagcgactga
     8521 tgcaggtgct tggcgtgatt gtagttcggt atcaggatcg agacggtcga cacggcatct
     8581 cctccgctgc agcatcgcac atatacgcga caacggctta tcgacggcgc gcggcgcttc
     8641 actaccacga ttgtctcggc acggtcttgt cgcgatatgt aggtgcagcc gattcctgtc
     8701 gatattccaa attgtcgccg aagcacgtcc gcaaaaaagt cacgccgcac gcgatgcgtg
     8761 caattcctga gggttggcac gacctgagat ggcatgtcac taggcaggtc gccgccaaga
     8821 cgggcgcgag tgccggactg cgcttgcctt attccaaggc ttcggtgatg agccagcgtg
     8881 tcagcaccgt tgcggcctcg aggaagtcat cgatctccat ggcctcgtgg gggttgtgac
     8941 tgccgttggc gttgcgcacg aacagcatcg ccgccggcac gccggccgtg gcaaaggtcg
     9001 cggtgtcgtg ggaagccgga cttgcgagcg gcatcgtcgc gatgccctgc gctcgtgccg
     9061 cgcccgtgag gcctgcgaga atatcccgat ccgagggtgc gacatccgca gtcgcgcggg
     9121 gacccatctc gaaggtgacg ccacgccgtg cctcgatcgc ttcgactagg gcaagcgtct
     9181 cgcgctcgag gttcgcgagg tgctccttgt cataggcacg cacgtcgagg ctcatcgcca
     9241 tctcgccagc gacctttgtc agctggtgct cgcgcggatt tgtgtggaag cgaccgatcg
     9301 tgaacgccat cggccggccg gctgcctcct cacgctgcca gatcgtatcg aggcccaagg
     9361 cgaagtcgct cgccgccatc accgcgtcgc gccggaaccg tcgcggcagg ccgacgtgcg
     9421 cgtgctcgcc gaggatgcgg atcgcgggat agcggaaatt gcccggcacg ccggtaccga
     9481 ccgctactgc gcgacccgcc tcgatcagct gcggcgcctg ctcaatgtgc acctcgaccc
     9541 aggcgcggat gccctgtggc tcgagcgagg ctttccgcgc tcgaacggca tcgacatcgg
     9601 caccgcaccc ggccatgtgc tcggcgagcg tgcgtcccgt gtcgatgcgg cgtgcttcga
     9661 gcgcgccatc agggagcgtg ccgaaagcgg accgcgagcc gatgtaggag acctggaacc
     9721 agacactctc ctcggcacga atcgccatca cggtgacgtc gcggcgtggc gtgagaccga
     9781 gccgcttcat cgcggcgacc gcgaccagcc ccgcgatcac gccggcggca ccgtcgaaat
     9841 tgccgccatt cggcacagaa tcgaggtgcg accctgtcat tacacggggc aagcgccgat
     9901 cggagccggg gagcgtcatc atcatattgg cgccgtggtc gcggcgcgtc tcgaggccga
     9961 gatcgcgcgc cttggcctcg atcacgtggt aggcgaactc ttcgccagcc ccgaacgtgt
    10021 cgcgcgtgac gcccgggccg accggatcga gacttcccgc acgcaactcg tcgaagagcc
    10081 gcgtggcctc gtcccgctcg gccagaactg aggtcttcaa ggcctgtgca tcgatctgca
    10141 tgtctcgtcc ggctcccgct tgcatggcgg aagcatagcg agcgcggcgc gtctccggca
    10201 attgccgaac tcagtcgggt tcactgcggc gagccgcgga acacgccatt cttgaaaaag
    10261 atccggaacg gcgtcgtacc tgtggcgcca ccctcgaagg cgcgccagcg cccgttccgg
    10321 cagaggacgc cgcccgccac ctcgaccgcg tcgaactcgg cgacgttggc gtggaccttc
    10381 ggttgggcgc cgggctcgac ggccatgaag gtcgtgatgc acttggcccc cgtgatgacc
    10441 ggatcatagc ccttgatcac gaagccgcca ccgccggtcg aaggaaacgt gctcttgagc
    10501 agcggccagc gctcgtagtt ctcgttggcg gcaagcggtg gcgcgagcag cgcggcagtg
    10561 actgcggcaa tcagcaggcg agcgcgcatg atcgtctccc gggtagtggc atcacatggg
    10621 agtacgctcg cgcgacaccc gttggatttc ggagaacagc cttccaccac aaggccgact
    10681 gctacccccg ttccatgccc ttcgacttga gcaccggcag cgtctcgggg ccggcgagga
    10741 gctggagcag cgcgcgagcg ccagccgcgg ccgtcgccgc gctcgcaagt ccgcccgaat
    10801 agatcgtgta gttctgcacc tcggccggca gcgggccgac gagcgtcacg cccttcacag
    10861 gcaggatctc actgatctgg tggacggcga gatcggcctc gcctgtcacc agtgcttcgg
    10921 cgacatagcc tccggccttg agcttggcct tggcccggac cggatctgcg atgccgagcc
    10981 gctcaagcag cctgtcgaaa tagatgccgc tggacccgcc cgctttcgga tcgatgtagg
    11041 tgaccgcgcg ggccgaggtc agcgcacgct tgaaggcatc gaccgaggcg atgtcgggaa
    11101 ccggcgcgcc cgtcttgacg gcgactccca tcccgacctt ggcgagatta atgcgcgtgc
    11161 cggcctgcac cttcccggac ttgataagcg catcgatgac ggccggcgtg atcacggcaa
    11221 gatcaaaggc ctcgccgccc tcgatccgct tggcaaggcc gcccgctgtg tcgttcttca
    11281 ccgagacctt gtggcccgtg gccttctcga agcctggcac catctcgagc accacagact
    11341 tcatggcgcc ggcggtcagc accttgatgt cttcagctcg agcgccttga gcgcttgcga
    11401 tggcgagcgt gagggtaaag cacaaggcgt gtagcgtgcg cattgcggtt ctccgaactt
    11461 taacggacag cttgatcctg gggcggttgg cgctcttcgc caatcacgct cgcgcgagcg
    11521 acggctcacg agcccggcat gacgagtgcc ttcgcgcgcg cccgcgcctc ggcgagcgtt
    11581 atgatgtcgc ccgtgtagcc gcccattgcc tcgtagtcgc ggcgtgccat cgtgaactcg
    11641 attggaaaaa cgattgccgg cgtcggcaca tagcccgtcg acctgtccgg aagcagcgac
    11701 acgtccacca tcaccgtgat gccgccgccc ggccagacat agctcggcgc gccgccgcag
    11761 gtgacgcgcg tcgcgcgctg tttcaccgag ttcgtgagcc gcaccgggtt ctcggtgacg
    11821 cctgcgcgca gcgatccgcc cgcgcctccc atgaagagca cgctcgacag cgccggctcg
    11881 caattctcgg caatgcgctc gatgagcttg gcaattcccg ccggcggctc ggccggcttg
    11941 ggcttcaagg cttcgtcgag cacgtaccaa ccccagtgct cgcccgtcgt cgagaccatc
    12001 agcaggcgga gcccgggcca cgcggtcttc gggtcgattg acttgatgat ctcgagcggg
    12061 tccttcacgt cggtgccgcc ccagccggag cccgggtgcg ccacctggaa atagcggccg
    12121 ggcgtcgagc ggcgcccgcg cacgcgaatg ccggagtgcg ggacatccag catgcggccg
    12181 gcctgatgct cggtgaagac gccggtgatg tgctcatcga ccacgatcac ctcgtcggcg
    12241 aggccatgcc actgcttggc gaagatcccc tgcaccgccg agccgcagcc gacgcgcatc
    12301 acggtctcgg tcttgccgcc gatgatcggc ggcttgccgg cggcgacgac gacactcgcg
    12361 ccgccgtcga tcgcaagctc caccgcctcc ttattgcaaa gccgcatgag cgcgtcgcag
    12421 gtgacgttac cctccgcctt cgagccgccg gtcaggtgcc ggacgccgcc gagtgagagc
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgc
    12541 cgcctgctca ggcccgatgt agcggtcggt atcgattttc accttcacgc cgcagtagga
    12601 gaagatgcct tccgtgacga ctgtcaccgt atcgacgccc tcgacctccg ccgagacgat
    12661 gaacggcgcc ggtttgtagt cgggataggt ggtcgtcgcg ccgatgcccg tgacgaacgt
    12721 gccgggcgtg cgcaccagcg tgccgtccca gtccgccgcc gcgccgagga aggcgacctc
    12781 gccgcgcccc gccgcccggt tgcgctcgag catgacgagc gggtcggtgc gcacgagccg
    12841 gccttccacg ttcccgtagc ggtcacaggc gccgagcttg cccggccgga tgcggcagag
    12901 caccgggcag gcatcacagc ggatgatgcc cgtctcggca tcccgctggc tggcatcgcg
    12961 gtcggcagca tcgctcatcg aggcctcgcg tttcgtttcc cacgagtttg ccacgccgca
    13021 ccagatagcg gaagcgtgcc tgcgagccat gctagagtgg cgactgctcc ctcttgcctc
    13081 cgctgttgac gctcgttttc gcagagtgcg atggttggcc cgcagcggca agggatcaat
    13141 gacatggcac agcgcgtcga cgcgaggacg ctcaagcagt ggctcgggga gccgggcgag
    13201 atcgccctcc tcgatatccg cgagatgggg ccgtactcgc aaggacaccc gttcctggcc
    13261 gtgccgctcc cctacagtcg cctggaggtc gagatcgagc ggctggtgcc gcgtcgttcg
    13321 gcacgcctcg tcgtgttcga cctgaagggc gagggcctcg ccgagacggc ggcaaagcgc
    13381 gccgaggcgc tcggatacac ggccgtccat gtgctcgccg gcggcgccga gggctggaag
    13441 gctgccggct acgagctgtt cgacggcgtg aacctgccgt ccaagacctt cggcgagatc
    13501 gtcgagatcg aacggcatac gccgcgcatt acggccgccg agctcgccgc catgcagacg
    13561 cgcggcgacg atatacagat cgtcgatggc cggaccatcg cggaatacca gcgcttctcg
    13621 attccgggcg gcgtcccttg cccgaacggc gagctcgcgc tgcgcatcgc cgatatcgtg
    13681 ccgagcccga aaacgaagat cgtcgtcaat tgcgctggcc gcacgcgctc gatcatcggc
    13741 gcgcagacgc tgatcgactg gggcgtgccg aacgacgtcg tagcgctgga gaacggcacg
    13801 caaggctggt tcctcgccgg cctaaagcgc gaggaaggtg cgacgggccg gatgccggca
    13861 ctgccgacgg gagcggccct cgagcgtgcc aaggctcgcg ccgcggcact cctggaggag
    13921 cgcggcgtgc cgacggtgga cgcacggacc gtcgagcgct ggatcggcga ggccgaccgc
    13981 acgacgtacg tgttcgatgt ccgcaccccc gaagagttca aggccgacgg catcgccggc
    14041 tccgtgtggg cggagggcgg gcagctcatc cagnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnncgtc gacggggaag gtgtccgcgc gccgatggtc gctgcctggc
    14161 tccgccagct cgggcatgat gcctgcgttc tcgccggcgg gcatacctcg ggcctgaaaa
    14221 tcgcgcgcgc cccggcaaag gccgaaaccg tcctgccgcc gcctcaccgg atcgaggcgc
    14281 gggcgctcgc cgccggcctc gaagctggca cggttgccgc attcgatctg cgcgcgagcg
    14341 ccgcctaccg cgacgcgcac attccgggca gctgctggtc gatccgcccg cgcattgctg
    14401 ccgacgcgtc agccgcgaaa ggcaagcgca tcgtgctcat cgccgatgag ggccggctcg
    14461 ctcgcgccgc ggcgattgat ctcgccgagg caggtcactc cgacgtcgcc atcctgcgcg
    14521 gcggcattga ggaatggcgc gccgaaggcc tggacctcgc ccgctcgccg ggagcaccgg
    14581 ccgacagcga gcgcatagac ttcatgtttc acacgctcaa ccgcaatgcc ggatcacgcg
    14641 ccgacgccga ggcctacctc gcctgggaag tcggccttgt cgatcgtctc gacgcgcagg
    14701 agcgcgccgt cttcaagctc tgggggaagc actgatggga aagtccaaga agcggccgca
    14761 gacgctgcgc acgaagctcg tccagggcgg ccggcccaag gagaaccagt acggccgctc
    14821 gcgcacggtc aacacgccga tctaccgggc gagcacagtc ctctacgaga acatggcggc
    14881 gctccgcgag acgcggaagc agcgcaccga gggcgagcgc gccttcagct acggtacatc
    14941 cggtacaccg accacgttcg cgctcgagga tgtggtgact gagctcgagg gcggccatgc
    15001 aacgcgcctc gtctcgtcgg gcctcctcgc catcacactc gccttccagg ccgtgctccg
    15061 cccgggcgat cacgtgctga ttaccgatag cacgtacgag cccgtgcgga acttcgtgcg
    15121 cacctggctc acgcccatgg gcatcgcaca cgactatttc ccggccgacg ccaggggcct
    15181 cgagggcatg atccggcgca atacgaaaat gatctggacg gagatcccgg gctcccacct
    15241 cttcgagatg caggacctgc cggcgatctg ccggatggcc aagcagcgcg gcattcttgt
    15301 cggcgtcgac aatacttggg cctccggcat cctctgccag ccgctgaagc tcggcgcgga
    15361 catctcgatc cttgcaggca cgaagcacat cgccggccac gccgacgtgc tcatcggcac
    15421 tgtgacgaca accgagtcat gctggaagcc cgtcaacgac atggcgagcg cgctcggcct
    15481 ctgcatcagc tcggacgacg cctttctcgc gctgcgtggt gtgcgcacca tggcaacgcg
    15541 gctgcccgtg caccagcgct cggcgttgga gctcgcaggc tggctcaaga agcatccgaa
    15601 ggtgaagagc gtgttctatc cggcgctgcc ggacgatccg ggccacgaga tctggaagcg
    15661 cgatttcacg ggtgcctgcg gcctcttctc gatcgagctc aaggccgatc aggcaggcgc
    15721 cgaccgtttc gtcgatggac tgacgctgtt cggcatcggc gcgtcgtggg gcggcttcga
    15781 aagtctcgtc atgccgagca acatcccggg cgcgcgtacg gccaccgact ggtccggacg
    15841 cgggccgatc ctaaggctcc atgcgggcct cgaggatccg tccgacctga tcgccgacct
    15901 ggaggcagcg ctcgcgctcg tctgaaaacg gaagaggtgc ccatgcccgc gtcgccgcga
    15961 ccgttcactc tcgccgttcc cgaggcggac atcgctgacc tctttgcgcg gctcgcgcgc
    16021 acgcggctcc ccgatcaggc gccggacggg ccgtgggcgt acggcaccga cgtcggctat
    16081 cttggccggc tcatcgagca ttggcggacg cgcttcgact ggcggcattg ggagggccgg
    16141 ctcaacgcgt tcccgcaatt caaggtggca ctcgacggta tcgacctcca ttacctgaac
    16201 gtgccgggaa agggccccgc gccgaagccg ctgctccttc tccacggctg gccgggctcg
    16261 gtgttcgagt tcgtcgacat catcccgcgc ctcactgatc ctgccagctt tggcggcgac
    16321 gcggccgacg cgttcacggt gatcgcgccc tcgctgccgg gctatggcct ctcgttcgtc
    16381 gccggtcaga agcgcttcgg catcccggca atggccgact gctttgcgcg gctcatgacg
    16441 gacgtgctcg gctaccggaa gttcgcggcg cagggcggcg actggggcgc attcacctgc
    16501 tctgccatgg gcgtgcgcca tccggagaag ctttctggaa ttcatctcaa catgctctcg
    16561 gtgagcccat atctgccgcc acccgcgaac ccgaccgacg ccgagcgccg ctaccaggac
    16621 gaaatccggg cctgggccaa ggaggagacc ggttacatcc agatccaggg aactcggccg
    16681 cagacgctgg cctttgcgct gaccgacagc cccgcggggc tcgccgcctg gatcgccgag
    16741 aaattccacg tgtggaccga tcacgccggc gacttcgaga cggtcgtatc gcgcgatcac
    16801 ctgctcgcga acatctcgct ctactggttc acgggcgcca tcggctcctc gttctggccc
    16861 tattacgagc gcctgcacgg cccctggccc atacccgacg gaaagactgt cgacgtgccg
    16921 ctcggctatg cggccttccc gcgcgagatc cgccgcccgc cgcgctcgct cgccgagaag
    16981 agctacactg acatccgcca atggaccgag atggcgaagg gcggacattt cgcggccatg
    17041 gagcagcctg aggcgctcgc gcgcgacgtc caggcattct tccgcccgct gcgcggcacg
    17101 ttctgaagca acgcatcagc tgccgtaacg gtcgtccaga ccctgatggt gggaaagccg
    17161 caccacctcc gtgaactcgg catagctcat ggtcttcccc gcgatccccg cgagcgaccc
    17221 tgagaccttg atcgccgcga gcgctgcttc gaccgcacga gccgctgtga acagcgccgc
    17281 gaccgcatag agcacgacgg cgaagcccat atcctgcagc tcgcgcgtcg tgagcagcgc
    17341 cgcgtccgtg ccgtcgacca gactcacgac cttcgggcca gcgacgcggc gggcaatgcc
    17401 ctcgacctcg gcgcgcgtct tgacaccatc gacgaacagg agatctgcgc cggcatcgcg
    17461 atagagggca gcgcgcctga cagcttcatc gagcccggtc gccggcagcg cgtccgtgcg
    17521 cgcgatcacg agaaggcgct cggccgtgcc gcgcgcggca agcgcgcatc tgagacgccg
    17581 cgcattctct gccgcatcaa tcagccggat gccggccatc tggccacagc gcttcggcgc
    17641 cacctggtcc tcgagatgca cggcggcgac gccggccgcc acatactctt caattgtccg
    17701 ctcgatgttc gacggcccac cgtaacccgt atcggcatcc gcgatcacgg ggagcgacgt
    17761 tgcccgcacc atgtcgcggg cgtgccccgt catctcggtc tggctcatca ggccgatgtc
    17821 aggcatgccg agccggctcg ccgtcgcgcc gaagccggtc atgtaaacgg ctgggaagcc
    17881 ggcgcgctcg acgaggcgcg cggtcagcgc atcgggtgcg ccaggggcga ccacgatcgg
    17941 tccggctgcg atgaggcggg aaagggccgt tgccttgctc atcgggtctc gctccgcctg
    18001 ctcatgacgc gtgatctcgc cgcagcagcg tacgcaacgt caatcggtcc gtgcggcatc
    18061 ggtggcgtca attctggtcg tcaggcagca caggcaccgg atagacctcg atgccgtcct
    18121 cgcgcagcgc ctcgacgtcg ctcttggacg cttcgccgtg gataccgcgc gcgggtgtct
    18181 ccttgaggtg gatcttgcgc gcctcgtcgg cgaatttcgg gccgacatac tccgacttcg
    18241 agaggagctc gtcgcgcact ttccgcatct ggcggagcag ctcgcgggtc tgccgcgcgt
    18301 ccggctcgcc tggtgccggg gccggactta tggccagttc cggaggcgcg gtttccgtgg
    18361 ttggcgccgg ttcggccggt ctgccgcgcc ccgacttccg gcccttgacg atgctcggcg
    18421 ccatgatcga ccgctcgacc ttggtcgagc cgcatttcgg gcactcgacg aggcgacgct
    18481 tcgcctgccg atcgaaagca tcggacgact tgaaccagcc ttcgaactcg tggcgcttat
    18541 cgcagcgcag tgtatagcgg atcatggctc gaactctagc atcctccagc cgacccgacg
    18601 agagcgaggc gagcgccggc gcttaccatt caacacgagt gccggtgact ggacaatgaa
    18661 cgattgccgc acgaccgaat tgcatctgca tattcgaggt tcgcttgatg gacgcgctgg
    18721 cctcgcgcgg ctgacgtggc gcagtgtagg ttcttggagc ggtctggatg cgaaagccct
    18781 gccggccccg gagcagcact catgtcgtcg ctaccgttag cggaggagtg atggcaatgc
    18841 acgatacccc gccgaagcac ttcgccgagc catctccctt cgtcgcccgc tggctcgagg
    18901 gcgtgggggg cagcggcacc gttctcgacg tcgcctgcgg cggcgggcgc cacctccggc
    18961 tcgcactcgc ccgtgggcat cgggtgacgg gggtcgatcg cgacatttcc ggcgtcgccg
    19021 acctcgccgc cgagccgcgc gcaacgatca tcgaggccga tctcgaaggc ggcgccggcc
    19081 tcgccccgca tcttgccggg caccgcttcg atggcgtgat cgtcgccaat tatctctggc
    19141 ggccgatcct cggggacatc attgcagcgg tctcatccgc gg
//
LOCUS       DAORNH010000045        19149 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2209_length_19149_cov_2.734210, whole genome shotgun sequence.
ACCESSION   DAORNH010000045 DAORNH010000000
VERSION     DAORNH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19149
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_19149_cov_2.734210"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PK264_06780"
     CDS             <1..412
                     /locus_tag="PK264_06780"
                     /inference="COORDINATES: protein motif:HMM:NF021376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2076 family protein"
                     /protein_id="HRD75624.1"
                     /translation="APTPPPALAPQPWGNASTGFSGRGAGPQPAPQPMPMMTPPPAAA
                     PAAKSGGFFKGALAAAAGVAGGVLLANSLGGMFGGSSANAGTPGGTGGGSENSGMTGQ
                     SDDSNDPGNYDAGSDDSDQGSDSWGSNDSSDIDL"
     gene            complement(477..2282)
                     /locus_tag="PK264_06785"
     CDS             complement(477..2282)
                     /locus_tag="PK264_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRD75625.1"
                     /translation="MWSLRRSLVLLFVAIVAMPLSLFWAWPHSASVKSELDEVRERHL
                     LLARNLGVTLSAYHRDVLASFNFLSETLLEGGGLPEPRTMLAGLRIRHICLVDPTDGR
                     VIVGYLGADRCPKELSKSDLARAIDLAKLGGTQFAPVHQGSDGSPTVSVVRLGSRGLV
                     IAELDARYFAELGKRISFGENGHAVIVDQTGTVLAHPMQSWEKAMRNIAAVPVVQKMI
                     MGKTGVDTFFSPAYKDTMVAGYTSVPGPGWGIMVPQPMRELKAAAARSRSSAVSVFAF
                     GLLAAALIACRAAILLIDPVRRVIVAAERMSNGDANVRIDVSGRFIPREMNALVQGFN
                     AMAERVSASRLAEAEARRTAERASQSKSEFLNTVTHELRSPLNAIIGFADIMASGRHG
                     PIGDERYRTAAEDVRSASRHLLALTNDLLDLARIEAGQFTLADDVFDLDEVTSRAARF
                     VEVEAQRRGTRIEVSHPDGQLTVAADERIMFQSTLNLVVNAVRYGGDNGHVRISAERT
                     ADGGVAISVADDGPGIGPEDLERVLRPFARTKSSERLTSPGSGLGLPIVAKLAELHGG
                     RLELASAVGVGTTATVYLPAKRLVASNPGLIKSAA"
     gene            complement(2528..3481)
                     /locus_tag="PK264_06790"
     CDS             complement(2528..3481)
                     /locus_tag="PK264_06790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRD75626.1"
                     /translation="MQLDMTPRKGTATAGMRPDSQIRDYDLVRQAIAFLTETWAEQPS
                     LEALAGHLALSPAHCQKLFSRWCGLSPKEFVQAITIDHARGLLAGSASVLDTAHEVGL
                     SGPSRLHDLFVTHEAMTPGAYKRRGEGLEIAWGVHPSPFGLALAMATGRGICGLAFFD
                     SESEFPHAFNDMSQRWPAAHFLEAPERTGTLLRNVFSPPEARGGEPIRLVLIGSNFEV
                     GVWEALLRIPMGRAVSYSDLARHVGRPSAARAVGSAVGRNPISFVVPCHRVLRGDGQL
                     GGYHWGLTRKRAIIGWERGRQAHPSPPAGIKAGAETKANDA"
     gene            3647..4216
                     /locus_tag="PK264_06795"
     CDS             3647..4216
                     /locus_tag="PK264_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclin-dependent kinase inhibitor 3 family
                     protein"
                     /protein_id="HRD75627.1"
                     /translation="MTDRGGLGSRRPAPLRIDEVRVPMGGVIGLCHCPGRNDGTRAWD
                     RDIAQDLKAIEDWGASHLVSLVQTREFARLGVPDLPERARASAFAWHHVPIRDLDVPG
                     SEAARQWPATETAIVETLRRGGRVVVHCAAGLGRTGTVAARLLVDHFGLAPDEAITSV
                     RAARPGTIETGDQEAYVRRSRLPPDPGVG"
     gene            4351..5214
                     /locus_tag="PK264_06800"
     CDS             4351..5214
                     /locus_tag="PK264_06800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75628.1"
                     /translation="MAASSGASPTAGETGGAQDQGPERSSRLDGRAALVIGAGSVGPG
                     WGNGKASAVAYARAGAKIAALDVNKAAAEETAAIIAREGGVAIAIAADVTRLDDVERA
                     VAAARATLGAIDILHNNVGIAKMGGPVELSEAEWQRVIDVNLTAMFRTTKVVLPIMQG
                     QKRGVITNISSVAAIRYVGYDYASYYASKGAVNQFTVGLALQYARAGIRANVIMPGLM
                     DTPLIYQQISGVYRSPEEMVKARNEACPMGRMGTGWDVANAAVFLASEEAQYITGVCL
                     PVDGGHSARSA"
     gene            5211..5972
                     /locus_tag="PK264_06805"
     CDS             5211..5972
                     /locus_tag="PK264_06805"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75629.1"
                     /translation="MTASLDEVARRGIVALEERGFAAWPALMTVLDDGWVIRLSRGHT
                     KRANSINVLGPSSEPLAEKCARAEALLAWHGLPIVYRLSPLAPAELTALLDQRRYELI
                     DDTRVMVADLKPSAERPDPRVSVRAGVAPDWTAAYAAADRVSVDRAATLQQMMASIAP
                     PHATAEVVDNGHRVAYGLGVVDRGMIGMFEILVAPSHRRQGLAAAIMAGLLDWGRRNG
                     ARRSYLQVVASNSPAIALYERLGYREVYRYHYRVA"
     gene            5979..7301
                     /locus_tag="PK264_06810"
     CDS             5979..7301
                     /locus_tag="PK264_06810"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03860.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HRD75630.1"
                     /translation="MPLSEKIHLAQFLVHGPTYHSLAMWRHPRTAAAGYDWRRPELYQ
                     HIAKVCERGLFDMVFFADLNYISDTFRGSLEPALRYAVQAPEHDPIPLLSWMAAVTSR
                     IGVAATFSTSQHHPFYAARMWATIDHLTAGRAGWNVVTSINHNQDANFGVDRPPTDAR
                     YERAHEFMQVCQKLWSSWDDDALVMDREKAIFADASKVRRIEHVGTHFKSRGPLNVVP
                     SPQRGPAILQAGTSPKGQDFAARYADAIFAIQPRAEDARRYYCSIKDQMAVFGRDPAH
                     CRILFGMQPIIGKTRAEALDKQAEHNALVPVDGGMAILSAHLDFDLARLPPDALMLER
                     TEPELARMRTRFVKPDGSSMTAAEVARRHGEGVGLPQFVGTPAEIVDQMEAFMATAGG
                     DGFMLSPIYSPGAIEGVVDLIVPEMQRRGLYRTAYKGTTQRDHLRQSD"
     gene            7331..8125
                     /locus_tag="PK264_06815"
     CDS             7331..8125
                     /locus_tag="PK264_06815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75631.1"
                     /translation="MAKAAKSRDYGHTVGAALVTGGASGIGRATVDLLIAKGWKVAAF
                     DIDERQLAELAETHDRSKVLLRTGRLDVTDERAVERAVAESAEAFGGLSGVVNSAGIA
                     ADKHVFETTPEMFRKILDVNVVGSFMVGKAAARIMKDKGRGSVVNIASISGLRGSKGR
                     SAYGASKGAVINMTQVMANDLAPYGIRVNAVAPGPVETPMVKALHTAADRKLYERFIP
                     MRRYAEPGEIASVIHFLLDPTQSAYVTGEVIAVDGGYRGAGVIVRD"
     gene            complement(8142..8849)
                     /locus_tag="PK264_06820"
     CDS             complement(8142..8849)
                     /locus_tag="PK264_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75632.1"
                     /translation="MTALLDVDNIHTAYGSSRVLFGISLSIAKGECVCLLGRNGVGKS
                     TTMRSIMGLTPPSSGAIRWKGQDVTRRQPYWMARNGIGYVPEDRRIFAELTVWENLDV
                     AQRSAPRPGPWTMEAVYGLFPKLAELRDRQGGVLSGGEQQMLTIARTLMGNPELLLLD
                     EPSEGLAPVIVENMLVQIQELKRQGLTILLAEQGLDFSLALADRVYVLQKGQVEFSGP
                     SQRLRDDAELRHKLLAV"
     gene            complement(8846..9583)
                     /locus_tag="PK264_06825"
     CDS             complement(8846..9583)
                     /locus_tag="PK264_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75633.1"
                     /translation="MLAAKNLRKSFSGFVATNDVSLTVERGQIFAVIGPNGAGKSTLF
                     NLITGHLTPDTGTVTLDGADITGVAPHQICRMGMARSFQRTNIFQRLSVYENIQAAFI
                     AFGGQSLNMWGTSRSQFRDKTEAMIAQLGLGDVAYSTAGVLSHGAQKQVELAIALAGE
                     PTILLLDEPTAGMSAQETHEAIALLARIAKERGLTLLFTEHDMDVVFGIAQRIAVLHQ
                     GRIIASGLPDEVRANAEVRRVYLGASH"
     gene            complement(9591..10610)
                     /locus_tag="PK264_06830"
     CDS             complement(9591..10610)
                     /locus_tag="PK264_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD75634.1"
                     /translation="MNDLVNRLPWTLILTLIALTIPFYTTRYYTDLATYIVIFAVFAT
                     SLNILIGSTGLVSFGHAAYFGIGAYVCGILMGDYNVPFLIAWVLGGLAGALFAAFFGF
                     FCVRLTATYFSMLTLAFSQIVWAICFKWNSVTGGEQGYPRVPYPGMDWMEAIPLIGSM
                     RVTEKFFFIALIMSAICIAAMRRIVDSPFGRILNAIRDNPERTQFIGIDVRRYRWIAF
                     VLAGAFAGLAGAMFGIYKRGVFPDYAYWAKGAEVLIMTILGGIHNFWGPTVGATALIL
                     LNGVIVSYTEYWPFVLGVILLILLFAFPGGILGTLDTLVRKPEERRAMLRSIGRTLRI
                     TRAGS"
     gene            complement(10607..11545)
                     /locus_tag="PK264_06835"
     CDS             complement(10607..11545)
                     /locus_tag="PK264_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD75635.1"
                     /translation="MSDILGLLAGEWQRITTTLSDPVFLISQFRTGLTSAMFLFLIAS
                     GLSLIFGVLKIINFAHGSMYMIGAYMAWYMTLILSKTTLGFAGAVLVAASTVALIGLV
                     VERFLLQFMYRREELYQLLFTYALVLIFGDACKYLWGTEPQSVSRPAALAGSWEMFGV
                     RLPHYDLMILIIGPAIAFGFWLLLNRTGYGRIIRAAALDREMLGALGTNVSRLYSGMF
                     VLAAFLAGLAGALVTPVKSIVPGMDVEIIIEAFIVVVIGGLGSLWGTLLGSLVYGLVL
                     AFGIVIFPRFSIFAAFIIMAAVLIVRPWGLLGRPVK"
     gene            complement(11724..12941)
                     /locus_tag="PK264_06840"
     CDS             complement(11724..12941)
                     /locus_tag="PK264_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75636.1"
                     /translation="MSSSYQLTRRSLLASTAAATGALIAPNAFGQGKPIKIGVPMPLS
                     GPAALFGVPAVKGMEMFVAETNAAGGLLGRPLQLLVRDSKATPDEAVRVAREMILKED
                     VNFLVGTLTSAEGPAVSTIAKENKIVLIAPIPKTDQLTAPKNLHPYIFRTAANTTMEG
                     RSSAEIMAKWTDVKTVATIAHDYAYGQDVAREFATHIKKLRPDINVVDQQWPKLGESD
                     YTPFINAQMAKKPDAVFSAMWGGHFVTWTKQAKPLGFFDLVKYRVVNGGEGGSVEATR
                     GLAGDYPFGIWSNTYDLASYEGMSAAHKAYVERIKAFTKEDPPSTWPITTYVALQFLA
                     AAVNKVKDVDNDKVAKALLDISVDAPFGKQTIRAKDHQADRGQFYGKMVKGPTGTAIM
                     TEVRYVDPKPFMD"
     gene            13041..13925
                     /locus_tag="PK264_06845"
     CDS             13041..13925
                     /locus_tag="PK264_06845"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75637.1"
                     /translation="MAERISIQFSRFSAFYSPLIATIAGGFLAEEGFAPEHSVAPVGK
                     SAIAGLVDGSVHVAQSAISQGFMSLEKGETPPVVHFAQINERDGFFIAARNPDPHFTW
                     AKLHGGRVLADHGGQPLAMFRYACKCAGLDLSRMTIVDAGINTMEARFRAGEGDYIHL
                     QGPGPQQLQHDGVGHVVASVGATFGPVAFSSLAARREWLKSEQAPRFMRAYRKARAWV
                     RTTPAAEIAAREAPFFPGVAREVLTATIDAYQKLGCWTPHVEITRPAFEVALDVFENA
                     KLVTRRHRYDDIIVPPPA"
     gene            complement(13932..14168)
                     /locus_tag="PK264_06850"
     CDS             complement(13932..14168)
                     /locus_tag="PK264_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75638.1"
                     /translation="MAQIVNFAPSKPGHVNPVLWQQSVGMARQVCARIFRDGGSPKTA
                     VEAFGLKSEPGTDWSRAVGAIAESLSARPMRRAA"
     gene            complement(15156..16244)
                     /gene="folP"
                     /locus_tag="PK264_06855"
     CDS             complement(15156..16244)
                     /gene="folP"
                     /locus_tag="PK264_06855"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRD75639.1"
                     /translation="MAEIYVRPVGRVSPAPKRAIGHPERVVRIAGRQDLAFSAFEAIE
                     RHGDGWVDRRQISLDDTTALLQSSRSSTDRLKTLLERISAPRGDVAGLPLDRPRIMGI
                     VNVTPDSFSDGGRHFSPKAAIEHALKLEAEGADILDIGGESTRPGSDAVPIDVELKRV
                     VPVLQGLAGKVRARLSVDTRKAEVMREAARAGAHILNDVSALSHDPVSLRAAADTGLS
                     VVLMHAQGGPKSMQANPRYADVVLDVFAALEERVEACVRAGIPRQKLIADPGIGFGKT
                     LEHNLQLLASLTLFHALGTGLLVGASRKSFIGALTGAVDPDERLPGSLAAACMAAGAG
                     AQIIRVHDVAATRQALTTWEAMSAGQAP"
     gene            complement(16321..17526)
                     /locus_tag="PK264_06860"
     CDS             complement(16321..17526)
                     /locus_tag="PK264_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HRD75640.1"
                     /translation="MPRLLLTSSAAAIAALALTTAAAVAQIAVSAQDAKVRLVDGKVT
                     TVKDGADNAVLIDLSSNPPKVIAELKLPASVVGPPSSVAVSPSEEIALITSAEKIDPA
                     DATKRVPNDQVTVIELNTKAPGVIAKVTARIKGATIEPPKPKVLSTLTAGKGAAGVSI
                     NRAGTLAIVANRGEGTLSVFSIKGKAVTALPDKVKIGDEKSGPSAIVFSADGKRALVT
                     RDGDNRISMLDIDGTKVTVNPRVITAGLRPYGADISGKGDVAVVANIGTGSGDADTIS
                     LIDLVANPPRVVITVTAGPTPEGIKMSPDGQYVAVTVMNGTNKPATDPFFKEKGKLVV
                     FRRAGLLLTDVGKWCQGIAWSKNGRTVVAQCMVEEMLFVFGFDGRQLRPMDPIRVKGG
                     PAGIRTAEK"
     gene            complement(17650..>19149)
                     /locus_tag="PK264_06865"
     CDS             complement(17650..>19149)
                     /locus_tag="PK264_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRD75641.1"
                     /translation="PILQDPALAFHPPFLYMGYVGFSMAFSFAVAALIEGRTDAAWAR
                     WVRPWTLAAWMFLTIGIGTGSWWAYYELGWGGWWFWDPVENASFMPWLAGTALLHSAV
                     VMEKREALKVWTILLAILTFSLSLMGAFIVRSGILTSVHSFALDPQRGMVILAIMAVF
                     IGGALTLFAMRAGALRQGGAFAPVSREGSLVLNNILLTVATATVLLGTLYPLMLEAIA
                     NQRISVGPPFFNMTFVPLMVPLLIALPFGPVLAWKRGDLAAAGQTLIAALIIAAAAAA
                     TAFAVNTRGPWLAPLAFGLGMWVIVGAAVEIATRIRLGRAPLDEALRRAAGLPRSAWG
                     TAFAHAGVGIMVLGIVGTSAWQQEVVASMKPGDRLSLSGTTIVFEGVSKQRGPNYQEL
                     IGRFVLIGRASATVRVEPSKRTFDAPRQTTTEAGIDARLGSDLYIVLGDEGKDGAFAV
                     RAYKNPLVRLIWIGAIVMFLGGLLSMSDRRLRVGAPKPSRRRSVAAPAE"
BASE COUNT         3408 a         6096 c         6553 g         3092 t
ORIGIN      
        1 ggctccgacc ccgcctcctg ccttggcgcc gcagccatgg ggcaatgcca gcaccggttt
       61 ctcgggccgc ggcgcagggc ctcagccggc accgcagccg atgccaatga tgacgccccc
      121 gccagccgcg gcgccggcgg cgaagtcagg aggcttcttc aagggcgcgc tggcggctgc
      181 ggccggagtc gcgggcggcg ttctgctcgc caacagcctc ggtggcatgt tcggcggctc
      241 gtcggccaat gccgggacac ccggcggcac gggcggagga tccgagaaca gcggtatgac
      301 cggccagtcc gacgatagca acgatcccgg caactatgac gccggcagcg acgactcgga
      361 ccagggtagc gacagctggg gcagcaacga cagcagcgac atcgacctct gatcgggtac
      421 tgcatggttc agccacgggg cgttcggatt gtctccgggc gccccatttt cgtggctcaa
      481 gctgctgatt tgatcaggcc ggggttcgat gcaacgaggc gtttcgccgg caaataaact
      541 gtcgccgtcg tgccgacgcc aacggcgctt gccagctcga gacggccgcc atgcagctcc
      601 gcgagcttgg ccacgatcgg gagaccgagg ccgctgcctg gagatgtcaa ccgctccgag
      661 ctcttcgtcc tcgcgaacgg gcgcaatacg cgctcgaggt cttcgggccc aatgcccgga
      721 ccgtcgtcgg caacgctgat cgcaacgccg ccgtcagcag tgcgctccgc cgagatgcgg
      781 acatggccgt tgtcgccacc gtagcgcacg gcgttcacga ccagattgag cgtggactgg
      841 aacatgatcc gctcgtcggc ggcgacggtc agctgcccgt cgggatggct cacctcgatg
      901 cgcgtgccgc ggcgctgcgc ctcaacctcg acgaagcgag ccgcgcgcga ggtcacctcg
      961 tcgagatcga acacatcgtc agcgagcgtg aactggccgg cctcgatgcg agcgagatcg
     1021 aggagatcat tggtgagggc aagcaggtgc ctgcttgccg agcgcacatc ctcggccgcc
     1081 gtccgatagc gctcgtcgcc gatcggcccg tggcggcccg acgccataat gtcggcaaag
     1141 ccgattatgg cgttgagcgg ggagcgcagc tcgtgcgtga cggtgttgag gaactcggat
     1201 ttggattggc tggcacgctc ggccgtcctg cgcgcctcgg cctccgcgag ccgggaggcc
     1261 gagacgcgct cggccatcgc gttgaagccc tggaccagcg cgttcatctc gcgcggaatg
     1321 aaacgcccgg acacatcgat gcgcacattc gcatcgccgt tcgacatccg ctcggccgcg
     1381 acgatgacgc ggcggacagg atcgatcagg aggatggcgg cccggcaggc gatcagggcc
     1441 gccgcgagaa gtccgaaggc gaagaccgag acggccgagc tgcggctgcg cgcggctgct
     1501 gctttcagct ctcgcatggg ctgcggcacc atgatgcccc agcccgggcc cggcacgctc
     1561 gtgtagccgg cgaccattgt gtccttgtag gccggcgaga agaacgtatc cacgcccgtc
     1621 ttgcccatga tcattttctg gaccaccgga accgcggcaa tgttgcgcat cgccttctcc
     1681 cagctctgca tcgggtgcgc gagcactgtg ccggtctggt cgacgatcac ggcgtgtcca
     1741 ttctcgccaa acgagatcct tttgccgagc tcggcaaaat agcgcgcgtc gagttcggcg
     1801 atcaccagcc cacggctgcc gagacggacg acgctgacgg tcggacttcc gtccgaccct
     1861 tgatgaaccg gcgcgaattg agttccgccg agctttgcca agtcaatggc acgagcaagg
     1921 tcgcttttcg acaactcctt cgggcaacga tcggcgccga gatagccgac gatgacacgc
     1981 ccgtcggtcg gatcgacgag acagatgtgc cggatccgaa ggcctgcaag catggtgcgg
     2041 ggctcaggca gaccgccacc ctcgagcaac gtctcggaga ggaaattaaa ggatgccagg
     2101 acgtctcgat ggtaggctga gagcgtgacg ccgagattgc gtgcgagcag caaatggcgc
     2161 tcgcggacct catcgagctc gcttttcact gaagccgagt gcggccatgc ccagaacagc
     2221 gacagcggca tggcgacgat cgcgacaaac agcaatacga gcgagcgtcg aagcgaccac
     2281 atagcaccca ccgactgatg gcccaaccac ggggagtatc gccggcaagc ttcgggaaag
     2341 aatgaatcgg gcgttcaatc aattgccgtg gtttcgaaag tctcggccga tccggtcaat
     2401 ctgcgatcaa ttacttctat tatttcaagt tcagtcgaga aagcgcgatt tcgcgctgcg
     2461 cgagagcgcc caattcgagg ctgaacggag tgtcctcgag attgatcgca aattgtcgga
     2521 aaatcattca tgcatcattt gctttagtct ccgcgcctgc ttttatgcct gcagggggcg
     2581 atgggtgcgc ctgacgacct cgttcccagc cgatgatggc gcgcttgcga gtcaatcccc
     2641 aatgatagcc gccgagctgg ccgtcaccgc gcaggacgcg atggcagggc acgacaaagg
     2701 agatcgggtt ccgcccgacg gcactgccga cggcgcgggc agcgcttggc cggccgacgt
     2761 gacgggcaag atcggagtag ctcacggcgc ggcccatcgg aatgcgcagg agcgcctccc
     2821 acacgccgac ctcgaaattc gatccgatca gcacgagcct gatcggctcg ccgcccctcg
     2881 cttcgggcgg ggagaagaca ttgcgcagca gcgtgcccgt ccgctcaggc gcctcgagga
     2941 agtgcgcggc gggccagcgc tgcgacatgt cgttgaacgc gtgagggaac tcgctctcgc
     3001 tgtcgaaaaa ggcgaggccg cagatgccgc gacccgttgc catggcgagc gccaggccga
     3061 acggggacgg atgcacgccc caggcgatct cgagaccttc gccacggcgc ttgtaggcgc
     3121 cgggggtcat cgcctcgtga gtgacgaaga gatcatgaag gcggctcgga ccggaaagcc
     3181 cgacctcgtg cgccgtgtcg aggacgctcg cagagccggc gagtaggccg cgcgcatggt
     3241 cgatggtgat tgcctgaacg aactccttcg gcgagagccc gcaccagcgc gagaagagct
     3301 tctggcagtg cgcgggactc agggccaggt ggcctgcgag cgcctcgagc gagggttgct
     3361 ccgcccaggt ctcagtcagg aaggcaatgg cctggcgaac gaggtcgtag tcgcggatct
     3421 ggctgtcggg tctcatacct gcggttgcag tgcccttgcg cggcgtcata tcgagctgca
     3481 tgtcggcctc ccggtcgtgc gttccgtctc gctggccctt tcagtagcaa gtcggcggcg
     3541 acggctcgac ccgtttcgtg tgatgccgtt ggcgattgcg caagccgcgc acatcgtcgc
     3601 aaactcgggt ctgccgggac gcggcaacgg caacagcgag ggcgccatga cggacagggg
     3661 cggattgggg tcgagaaggc cggcgccgct gcggatcgac gaggtgcggg tgccgatggg
     3721 cggcgtgatc ggcctctgcc actgccccgg ccggaatgat ggcacgcgag cgtgggatcg
     3781 ggacatcgct caggacctaa aggcgatcga ggattggggc gcgtcccacc tcgtctccct
     3841 ggtgcagacg cgagagttcg cgcgcctcgg ggtaccggac ctcccggagc gggcacgggc
     3901 ctcggccttt gcctggcatc atgtgccgat ccgcgatctc gacgtgccgg gcagcgaggc
     3961 ggcccggcag tggccggcaa cggaaacggc gatcgtcgaa acgttgcgtc ggggtggtcg
     4021 tgtcgtcgtc cattgcgcgg cgggcctcgg tcgcacggga acggttgcgg cgcgcctgct
     4081 tgtcgatcac ttcggccttg cgcccgacga ggccatcacg agcgtgcgag ccgcgcgccc
     4141 cggcaccatc gagaccgggg accaggaggc ctacgtgcgc cgatcgcgcc ttccgcctga
     4201 tcccggcgtg ggttgatctc gaatgcggtg cgggacgcgc acgggacgct cagccgctgg
     4261 acgctggagg ctgatcccac gtagtctcgc ttaagggttc ggcggcagcg aacgccggtt
     4321 cgagtgccgc gcaatgaggt ggacagtgac atggctgcaa gcagcggggc aagcccgaca
     4381 gcaggcgaga caggaggcgc tcaggaccag ggcccggagc ggtcgagccg gctcgacgga
     4441 cgcgcggcgc tcgtcatcgg cgccggctcg gtcggacctg gctggggcaa cggcaaggcg
     4501 tcggctgtgg cctacgcgcg cgcaggtgca aaaatcgcgg ccctggacgt caacaaggcg
     4561 gcggccgagg aaaccgcggc gatcatcgcc cgcgaaggcg gggtcgcgat cgccatcgct
     4621 gccgacgtca cgcgcctgga cgacgtggag cgcgctgtgg cggcggcgcg cgcgacgctc
     4681 ggggcgatcg acatcctgca caacaacgtc ggcatcgcca agatgggcgg tccggtcgag
     4741 ctttccgagg cggagtggca gcgggtcatc gacgtgaacc tcaccgccat gttccgcacc
     4801 accaaggtgg tgctgccgat catgcaggga cagaagcgcg gcgtgatcac caacatctcg
     4861 tccgtcgccg ccatccgtta cgtcggctat gactacgcga gctactatgc ctcgaaaggc
     4921 gcggtcaatc agttcacggt ggggttggcg ctgcagtacg cgcgcgccgg catccgggcg
     4981 aacgtgatca tgccgggcct catggacaca ccgctgatct accagcagat ctcgggcgtc
     5041 tatcggtcgc cggaagagat ggtgaaggcg cgcaacgagg cctgccccat ggggcgcatg
     5101 ggcaccggct gggacgtcgc caacgcggcc gtattcctcg cgagcgagga ggctcaatac
     5161 atcacgggcg tctgcctgcc ggtcgacggc ggccattcgg cgcgcagcgc atgacggcct
     5221 cgctcgatga ggtcgcgcgg cggggcatcg tcgccctgga ggagcgcggc ttcgcggcct
     5281 ggcccgcgct catgaccgtg ctcgacgacg gctgggtgat ccgcctgtcg cgcgggcata
     5341 cgaagcgcgc caactcgatc aatgttctcg ggccatcgtc cgagccgctc gcggagaagt
     5401 gcgcacgggc cgaagcgctg ctcgcctggc acggtctgcc gatcgtctac cggctgtcgc
     5461 cgctggcccc tgccgagctg acggcgctgc tcgaccagcg ccggtacgag ctgatcgacg
     5521 atacgcgcgt gatggttgca gacctgaagc cgagcgccga gagacccgat ccgcgcgttt
     5581 ccgtccgggc gggcgtcgct ccggactgga ccgcggccta tgcggcggca gatcgtgtca
     5641 gtgtcgaccg cgcggcgaca ctgcagcaga tgatggcctc gatcgcgccg cctcatgcga
     5701 cggccgaggt cgtggacaac ggccaccgcg tcgcctacgg gctaggcgtt gtcgatcgcg
     5761 gcatgatcgg catgttcgag atactcgtcg cgccgtcaca ccggcgccag ggtcttgccg
     5821 cggcgatcat ggcgggcctg ctcgactggg gacggcggaa cggtgcgcgg cgctcatacc
     5881 tgcaggtcgt cgcaagcaac tcgcccgcca tcgcactcta tgagcgtctc ggctatcgtg
     5941 aggtctatcg ctaccattat cgcgtggcct gacaaccaat gccgctcagc gaaaagatcc
     6001 acctcgccca gttcctcgtg cacggtccga cctaccacag cctcgccatg tggcggcatc
     6061 cccgcacggc ggcagcgggc tacgactggc gccggcccga actctaccag cacatcgcca
     6121 aagtgtgcga gcgcggcctc ttcgacatgg tcttcttcgc cgatctcaac tacatttcgg
     6181 acacgtttcg cggttctctg gagccggcgc tgcgctatgc cgttcaggcg cccgagcatg
     6241 atccgattcc gctgctctcc tggatggcgg cggtgacaag ccgcatcggg gtcgcggcca
     6301 ccttctcgac aagccagcat catcccttct acgccgcgcg gatgtgggcg acgatcgatc
     6361 acctgacagc tggccgcgcc ggctggaacg tcgtgacgtc catcaaccac aaccaggatg
     6421 cgaacttcgg cgtagatcgt ccgccgacgg acgcgcgcta cgagcgtgcg cacgagttca
     6481 tgcaggtctg ccagaagctc tggtcgagct gggacgacga tgcgctcgtc atggatcgcg
     6541 agaaggcgat ctttgccgac gccagcaagg tccgccgcat cgagcatgtc ggcactcact
     6601 tcaagtcgcg agggccgctc aatgtggtgc cgtcgccgca gagggggccg gcgatcctgc
     6661 aggcgggcac atcgccgaaa ggccaggact tcgcggcgag atatgcggac gcgatcttcg
     6721 cgatccaacc gcgcgccgag gatgcgaggc gctactactg ctcgatcaag gaccagatgg
     6781 cagtgttcgg ccgtgatccg gcgcactgcc gcatcctgtt cggcatgcag ccgatcatcg
     6841 gcaagacgcg ggccgaagcg ctcgataaac aggccgagca caacgcactg gtgcccgtcg
     6901 acggtggcat ggcaatccta tcggcgcacc tcgatttcga tctcgcgcga ctgccgccgg
     6961 acgccctcat gctcgagcgc accgaacccg agcttgcccg catgcgcacc cgcttcgtga
     7021 agcccgacgg ctcgtcgatg acggcggcgg aagttgcgcg ccggcacggc gaaggcgtcg
     7081 gcctgcccca gttcgtcggc acgccggctg agatcgtcga ccagatggag gcgttcatgg
     7141 cgacggcggg cggcgacggt ttcatgctct cgccgatcta ttctccgggt gccatcgagg
     7201 gggtcgtcga cctgatcgtg cccgagatgc agcggcgcgg gctctatcga acggcctaca
     7261 aggggacgac gcagcgcgat catctgcgcc agagcgactg acacgcaaca tcgggtctta
     7321 cggggacgac atggcgaaag cagcgaaatc cagggactat ggacacaccg tcggggcggc
     7381 gctcgtgacc ggcggcgcgt caggcatcgg cagggcgacg gtggacctgc tcattgccaa
     7441 gggctggaag gtcgcggcct tcgacattga cgagcgccaa ctcgccgagc tcgcggaaac
     7501 ccacgacagg tcaaaggtgc tgctgcgcac cggccggctc gacgtcaccg acgagcgggc
     7561 ggtggagcgc gcggtcgcgg agagtgctga agcatttggc gggctctccg gtgtcgtcaa
     7621 ctcggccggc atcgccgctg acaagcacgt cttcgagacg acgcccgaga tgttccgcaa
     7681 gatcctcgac gtcaacgtgg tcggctcgtt catggtcggc aaggctgctg cgcgcatcat
     7741 gaaggacaag ggccgcggct cggtcgtcaa tatcgcctcg atctcgggcc tgcgcggctc
     7801 caaagggcgc tcggcctatg gcgcgtcgaa gggcgcggtc atcaacatga cgcaggtcat
     7861 ggcgaacgac cttgctccct acggcatccg tgtcaacgca gtggctcctg gtccggtcga
     7921 gacgcccatg gtcaaggcgc tccacaccgc ggccgaccgc aagctctacg agcgcttcat
     7981 tccgatgcgc cgttatgcgg agccgggcga gatcgcctcg gtgatccatt tcctgctcga
     8041 tcccacgcag tccgcctacg tgaccggcga ggtgattgcg gtcgatggcg gatatcgggg
     8101 agccggcgtg attgtacgcg actgagcgcc ggcgacggcg ctcaaacggc cagcagcttg
     8161 tggcgcagct cggcatcgtc gcggaggcgc tggctcggcc ccgagaactc gacctggccc
     8221 ttctggagca catagacgcg atcggcgagt gccagcgaga agtccaggcc ctgctcggca
     8281 agcaggatgg tgaggccctg ccgcttaagc tcttgaatct gcaccagcat gttctcgacg
     8341 atgacggggg caagcccctc ggagggctcg tcgagcaaca ggagctcggg gttgcccatc
     8401 agtgtgcggg cgatggtcag catctgctgc tcgccacccg aaagcacgcc gccctggcga
     8461 tcgcgcagct ccgcgagctt cgggaagagg ccgtagacgg cctccatcgt ccaggggccg
     8521 ggccgcggcg ccgagcgttg cgcgacgtcg agattctccc agacggtgag ctcggcgaag
     8581 atgcgccggt cctcgggcac atagccgatc ccgttgcggg ccatccagta gggctggcgc
     8641 ctcgttacgt cctggccttt ccagcggatg gcgccgctcg agggcggcgt cagtcccatg
     8701 atcgagcgca tggtcgtcga tttgccgacg ccgttgcggc cgagcaggca cacgcactcg
     8761 cccttggcga tcgaaagcga gatgccgaac aggacccggc tcgagccgta ggccgtgtgg
     8821 atgttgtcga cgtcgaggag ggcggtcaat ggcttgctcc gaggtagacg cggcggacct
     8881 cggcgttggc gcgcacctcg tcggggaggc cggaggcgat gatccgtccc tgatgcagca
     8941 cggcgatgcg ctgcgcgatg ccgaacacca cgtccatgtc gtgctcggtg aagagcaagg
     9001 tgaggccgcg ctccttggcg atgcgggcga gcagcgcgat cgcctcgtgt gtctcctgcg
     9061 cgctcatgcc ggcagtcggc tcgtccagga gcagaatggt cggttctccg gcgagcgcga
     9121 tcgccagctc cacctgcttc tgcgcgccgt gtgaaagcac gccggcggtc gagtaggcga
     9181 cgtcgccgag accgagctgg gcgatcatcg cctcggtctt gtcacggaac tggctccgcg
     9241 aggtgcccca catgttgagg ctttggccgc cgaaggcgat gaaggccgcc tggatgtttt
     9301 cgtagacgga gagccgctgg aagatgttgg tgcgctggaa tgaacgcgcc atgcccatcc
     9361 ggcaaatttg atggggcgcg acgccggtga tgtcggcgcc gtcgagagtg acggtgccgg
     9421 tgtcgggtgt cagatgcccg gtgatcagat tgaacagcgt cgacttgccg gcgccgtttg
     9481 ggccgatcac ggcgaagatc tggccgcgct cgacggtgag gctcacgtcg ttggtggcga
     9541 cgaagccgga gaacgacttc ctgaggttct tggcagcgag cattgcgagg tcagctccct
     9601 gcccgagtga tgcgcaacgt tcgtccaata ctgcgcagca tggcgcgccg ctcctcaggt
     9661 ttccgcacga gcgtgtcgag cgtgccgagg atgccgccgg ggaacgcgaa cagcagaatc
     9721 agcaggatga caccgagcac gaacggccag tactcggtgt aggagacgat cacgccgttg
     9781 agcaggatca gcgccgtggc gccgaccgtc gggccccaga aattgtggat gccgccgagg
     9841 atcgtcatga tcagcacttc ggcgcccttt gcccagtagg cgtagtcggg gaagacgccg
     9901 cgcttgtaga tgccgaacat ggcgccggcg agcccggcga atgcgcccgc cagcacgaag
     9961 gcgatccagc ggtagcggcg tacatcgatg ccgatgaact gcgtgcgctc gggattgtcg
    10021 cggatggcgt tgaggatgcg cccgaacggg ctgtcgacga tgcggcgcat ggcggcgatg
    10081 cagatggccg acatgatgag cgcgatgaag aagaacttct cggtgacgcg catggaaccg
    10141 atgagcggga tcgcctccat ccagtccatg cccgggtagg gcacgcgcgg atagccctgc
    10201 tcgccgccgg tgaccgaatt ccacttgaag cagatggccc acacgatctg cgagaaggcg
    10261 agtgtcagca tcgagaaata ggtggcggtc aggcgcacgc agaagaagcc gaagaaggcg
    10321 gcgaagagcg cgccggcgag cccgccgagc acccaggcga tgaggaacgg cacgttgtag
    10381 tcgcccatca ggatgccgca cacgtaggcg ccgatgccga agtaggcggc atgtccgaac
    10441 gagacgagcc cggtcgagcc gatcaggatg ttgaggctcg tcgcgaacac cgcgaaaatc
    10501 acgatgtagg tggcaaggtc ggtgtagtag cgggttgtgt agaacgggat cgtcagcgcg
    10561 atcagcgtga ggatcagcgt ccaggggagg cgatttacga ggtcgttcat ttcacgggcc
    10621 tcccgagcag gccccagggc cggacgatca agaccgcggc catgatgatg aaggccgcga
    10681 agatggagaa gcgcgggaaa atgacgatcc cgaaggcgag gacgaggccg tagacgagcg
    10741 agccgaggag ggtgccccac agcgagccga ggccgccgat gacgacgacg atgaaggcct
    10801 cgatgatgat ctcgacatcc attccgggga cgatactctt cacgggcgtg acgagcgcgc
    10861 cggcgaggcc cgcgaggaag gccgcgagca cgaacatgcc cgagtagagc cgcgagacgt
    10921 ttgtgccgag cgcgccgagc atctcgcgat cgagggcggc cgcgcggatg atgcggccat
    10981 atcctgtccg attcagcagc agccagaagc cgaaggcgat cgcggggccg atgatcagga
    11041 tcataaggtc gtagtggggc aagcgcacgc cgaacatctc ccaactgcca gcgagcgccg
    11101 ccgggcgcga gaccgactgc ggctcggtgc cccagagata cttgcaagca tcgccgaaga
    11161 tgagcaccag cgcatatgtg aagagcagct gatagagttc ctcgcgccgg tacatgaact
    11221 gaagcaggaa gcgctcgacg acgaggccga tcagggcgac cgtcgaggcg gcgacgagca
    11281 cggcgcctgc aaagccgagc gtggtcttcg agagaatcag cgtcatgtac cacgccatgt
    11341 aggcgccgat catgtacatg gagccgtggg cgaagttgat gatcttcaga acaccgaaaa
    11401 tcaacgacag accggacgcg atgagaaaca ggaacatcgc cgaggtgagg ccggtgcgga
    11461 actggctgat gaggaacacc gggtccgaca gcgtcgtcgt gatgcgctgc cactcaccgg
    11521 cgagcagacc gaggatatcg ctcatgctga agcgctccta gcgtgcgagc gcccttggcg
    11581 atcagccatg gctggtcgtc tcgtcatgct gatgtccccc gatgcaggct cgccacggtc
    11641 atgccgggct tcgcccgcgg atcgatccgc gccccacgca cgtcgaaaag gagcccgcta
    11701 ccccagcggg ctccggtcgt tcttcagtcc atgaacggct tcgggtcgac gtagcggacc
    11761 tcggtcatga tggccgtgcc cgtcggaccc ttgaccatct tgccgtagaa ctggccgcgg
    11821 tcggcctgat ggtccttggc gcggatggtc tgtttgccga acggagcatc gaccgaaatg
    11881 tcgaggagcg ccttggcgac cttgtcgttg tcgacgtctt tcaccttgtt gacagctgcg
    11941 gcaaggaact gcagcgccac gtaggtcgtg atcggccacg tagagggcgg gtcctccttg
    12001 gtgaaggcct tgatccgctc gacataggcc ttatgtgccg ccgacatgcc ctcgtagctg
    12061 gcgaggtcat aggtattcga ccagatgccg aacgggtagt cgccggcaag gccgcgcgtc
    12121 gcctcgaccg agccgccctc gccgccgttc acgacgcgat acttgacgag atcgaagaag
    12181 ccgaggggct tggcctgctt cgtccaggtg acgaagtggc cgccccacat cgccgagaac
    12241 actgcgtccg gcttcttcgc catctgcgca ttgatgaagg gcgtgtagtc gctctcgccg
    12301 agcttcggcc actgctgatc gacgacgttg atgtcggggc gcaacttctt gatgtgggtc
    12361 gcgaattcgc gggcgacgtc ctggccgtag gcatagtcgt gggcgatggt ggcgaccgtc
    12421 ttcacgtcgg tccacttggc catgatctcg gctgacgagc ggccttccat agtggtgttg
    12481 gcagcggtgc ggaagatgta ggggtgcagg ttcttcggcg cggtcagctg atccgtcttc
    12541 gggatcgggg cgatgaggac aatcttgttc tctttcgcga tcgtcgagac ggcgggacct
    12601 tcggccgagg tcagcgtgcc gacgaggaag ttcacgtcct ccttgaggat catctcgcgg
    12661 gcgacgcgca cggcttcatc cggcgtcgcc ttgctgtctc gtaccagtag ctgcagcggc
    12721 cggcccaata gaccgccggc ggcgttggtc tcggcgacga acatctccat gcccttgacc
    12781 gcgggcacac cgaagagcgc ggccgggcct gaaagcggca ttggcacgcc gatcttgatc
    12841 ggcttgccct gaccgaaggc attgggcgcg atgagcgcgc ccgtggccgc ggccgtcgag
    12901 gcgagcaggc tgcggcgcgt caattgatag gatgacgaca tggagcggcg tttcccttga
    12961 tttcggtctt gctgaccggt tgttcccagt cgaagctagg acgccgcgcg gtgatatgtc
    13021 aaagagcaaa gggtcgggcg atggccgaac gcatctcgat acagttttcg cggttctccg
    13081 cgttctacag ccctctcatc gccacgatcg cgggcggttt cctggcggag gaaggcttcg
    13141 cgcccgagca ttcggtggcg cctgtcggca aatcggcgat cgccggtctg gtcgacggca
    13201 gcgtgcacgt cgcccaatcg gcgatatcgc aagggttcat gtcgctcgag aagggcgaga
    13261 cgccgccggt cgtccatttc gcgcagatca acgagcgcga cggcttcttc attgccgcgc
    13321 gcaacccaga cccgcatttc acgtgggcga agctccacgg cggccgtgtg cttgcggatc
    13381 acggcggcca gccgctcgcc atgttcaggt atgcctgcaa gtgtgcgggt ctcgatctgt
    13441 cgcgtatgac aatcgtcgat gcgggcatca atacgatgga ggcgcgcttc agagcgggcg
    13501 aaggggacta catccacctg caggggccgg gcccgcagca actccagcat gacggcgtcg
    13561 gccatgtcgt cgcctcggtc ggcgcgacgt tcgggccggt cgcgttctcg agcctcgcgg
    13621 cgcggcggga atggctcaag tcagagcagg caccgcgatt catgcgggcc taccgcaagg
    13681 cgcgcgcctg ggtccgcacg acgcccgccg ccgagatcgc ggcgcgcgag gctccattct
    13741 ttccgggtgt ggcacgtgag gtactgacgg cgacgatcga cgcctaccag aagctcggct
    13801 gctggacgcc tcacgtcgag atcacacggc ccgcattcga ggtggcgctc gatgtcttcg
    13861 agaacgcaaa gctcgtgacg cggcggcacc gctatgacga catcatcgtg ccgccgccgg
    13921 cctgagcagc attaggccgc acgccgcatg ggccgcgccg aaagcgattc ggcgatcgcc
    13981 ccgacggcgc ggctccaatc agtgccgggc tcggacttca ggccgaacgc ctcaacggcc
    14041 gtcttcggcg agccgccgtc gcggaagatg cgcgcgcaaa cctgacgcgc catgccgact
    14101 gactgctgcc agagcacggg gttgacgtga ccgggcttcg acggagcaaa attgacgatc
    14161 tgagccatgg gggactgcct ctctgtcatg cgcggcctta atgccgcgtt ggttcttgcc
    14221 gggacgaggt ggcaggcacg cacagaacta gcatcacgac gcaaaacctt gaactgtgat
    14281 gccgcttggc tgtgctgagc cgcagctgtg cgtgcctgcc agaccgatga gggcgggggc
    14341 cctcgaattc gtcagcgcgg ttcgaacctc gcctgtctgt ctttgttctt gggttgcggt
    14401 gtgctctcgg cctgcacgga tgccaggagt gcggagacga ggtcaccgag gggacgccac
    14461 tcaacggact gaactggacg atagactgac tctactcgga tgcatgcgga cactggaact
    14521 ctccacggaa ccatgtttga cgcgacgcgg acgcaacaaa tggaacgtta agcagggaac
    14581 gctacggcac caacgcaaca ccgaacgcca tacgctacac gacgcaacac tcaccaacgc
    14641 gacgcagaac cagcagacac aaacacaaca gatacttgca ggacccgtgc acctacgaga
    14701 cgcattacct cgggcccgcg acgcattcac gacgacgcag cacgcgttcg agcggaaacg
    14761 ctgctcgagg gacgcagtac aaattggatg cacagcacaa cttttgagga cgcgacccgg
    14821 gcaaactcac gcgccaactc aggtcactgg cgccgccgat acacaggggc gccaacacag
    14881 cggtacggtc gcccatcggg tgtcgagcct tacgcgagcc gactgactta aggctcgagg
    14941 caatcaccta cgcggccatt gcctcaggta ctcgtcccct tgcgcccgct tggccgtcta
    15001 ctcgtactcg gccagctggg cgtcagggcc cgtccgctga tggtcttgat aaaccgttac
    15061 aatcgggcaa ctctagggca cgatcgtgga cctcgagggg tatttcgatt gacagatgtt
    15121 cagacggcac gaatccggcg tgtcgcgccg ccggctcagg gcgcctggcc ggccgacatc
    15181 gcttcccagg tcgtgagcgc ctggcgcgtc gcggcgacat catggacgcg gatgatctgg
    15241 gcaccggcgc cggcggccat gcaggctgcg gccaaggatc ccgggagccg ctcgtccgga
    15301 tcgacggcgc cggtcagcgc gccgatgaaa ctcttgcgcg aggcgccgac gagcaggccc
    15361 gtgccgagcg cgtggaagag ggtcaggctg gcgagtagct ggaggttgtg ttcgagcgtc
    15421 ttgccgaagc cgatgccggg atcggcgatc agcttctggc gaggaatccc cgcccggacg
    15481 cacgcctcga cgcgctcctc gagcgccgcg aagacatcta gcacgacgtc cgcgtaccgc
    15541 gggttcgcct gcatggactt agggccgccc tgggcatgca tgaggacgac gctgaggccc
    15601 gtgtccgcgg cagcgcgcag gctcacaggg tcgtggctca aagcggagac atcattgaga
    15661 atgtgagcgc ctgcgcgggc cgcctcgcgc atcacttcgg ccttgcgggt gtcgaccgac
    15721 aggcgcgcgc gcaccttgcc ggcaagtccc tggagcacgg gaacgacgcg tttcagctcg
    15781 acatcgatcg gcacagcatc cgagcccggt cgtgtcgatt ccccgccgat gtcgaggatg
    15841 tcagcgcctt cggcctcgag cttaagggcg tgctcgatgg cggccttcgg cgaaaagtgc
    15901 cggccgccgt ccgagaaact gtccggcgtg acgttgacga tccccatgat gcgcggccgg
    15961 tcgagcggca ggccggcaac gtcgcctcgt ggagccgaga tccgctcgag cagcgtcttg
    16021 aggcgatcgg tcgaggaccg gctcgactgc aggagcgccg tcgtatcgtc gagcgagatt
    16081 tggcgccgat caacccagcc gtcgccatgc cgctcgatcg cctcgaaagc gctgaacgcg
    16141 aggtcctggc gaccggcgat gcgcacgacg cgctcgggat gcccgatcgc ccgcttcggc
    16201 gccgggctga cgcgccccac tgggcgcacg tagatttctg ccaccatcaa ccccgagccc
    16261 gcccaacgct accccgcgcg gtcgaatgtg ggagcgagga accgctcccg cggggctcga
    16321 tcacttttca gcggtgcgaa tgccagcggg gccgcctttg acccggatcg ggtccatcgg
    16381 gcggagctgg cggccgtcga agccgaacac gaacagcatc tcctcgacca tgcactgggc
    16441 aacgacggtg cggccgttct tcgaccaggc aatgccctgg caccacttgc cgacatcggt
    16501 gagcaaaaga ccagcgcgac ggaagacgac gagcttgccc ttctccttga agaagggatc
    16561 tgtcgccggc ttgttggtgc cgttcatcac cgtgacggcg acatactggc cgtccggcga
    16621 catcttgatg ccctcgggcg tcgggccggc ggtgaccgtg atcacgacgc gcggcggatt
    16681 ggcgacgaga tcgatgaggc tgatggtatc ggcatcgcct gagcccgtgc cgatgttggc
    16741 gaccacggca acgtcgccct tcccagagat atcggcgccg taaggcctga ggccggccgt
    16801 gatgacgcgt gggttgacgg tcactttggt gccgtcgatg tcgagcatcg agatgcggtt
    16861 gtcgccatcg cgggtgacga gcgcccgctt gccgtcggcc gaaaacacga tcgcgctcgg
    16921 accggatttc tcgtcgccga tcttgacctt gtcaggcagc gccgtgacgg ccttgccctt
    16981 gatagaaaag accgagagcg tgccttcacc gcggttggcg acgatagcga gcgtaccggc
    17041 ccggttgatc gaaacgcctg cggcaccctt gccggcagtc aaggtggaga gcaccttggg
    17101 cttcggcggc tcgatcgtcg cgcccttgat gcgcgccgta accttggcga tgacccccgg
    17161 cgccttcgtg ttgagctcga tcacggtcac ctggtcgttc gggacgcgct tcgtcgcatc
    17221 ggccgggtcg atcttctcgg ctgaggtgat aagtgcgatc tcctcgctgg ggctcacggc
    17281 gacgctcgac ggcggaccga cgacacttgc gggaagcttg agctcggcaa tcaccttcgg
    17341 cggatttgag ctgagatcga tgaggaccgc gttgtcggcg ccatccttga cggtcgtgac
    17401 cttgccgtcg accaggcgga ccttggcatc ctgagcggag accgcgatct gcgcgacggc
    17461 ggccgcagtg gtgagcgcga gtgcggcaat ggcagcagcc gaactcgtaa gcagcaagcg
    17521 gggcatcagt cctcctcgac acaactgtaa gcagcccatg tggcccacac gtcataccat
    17581 cggggccgtc gatcgaccaa cgcctttgcg agcgttggcc gaacgtccga ccgccctcat
    17641 agcgcggcac tattcggcgg gtgcagcgac gctccgccgc cgtgacggct tcggcgcgcc
    17701 gacccgaagg cggcgatccg acattgacag caggccgccg aggaacatta cgattgcgcc
    17761 gatccagatg aggcgcacga gcgggttctt gtaggcgcga acggcaaaag cgccgtcctt
    17821 gccctcgtcg ccgagcacga tgtagaggtc gctgccgagt cgggcgtcga tacccgcttc
    17881 cgtggtcgtc tggcggggcg catcgaacgt gcgcttcgag ggctctacgc ggaccgtcgc
    17941 actcgcccgg ccgatcagca cgaagcgccc gatcagctcc tggtagttgg ggccgcgctg
    18001 tttgctgaca ccctcgaaca cgatggtcgt cccgctgagg ctcagccggt cgccgggctt
    18061 catggaggcg accacctcct gctgccaggc gctcgtgccg acgatgccga gcaccatgat
    18121 gccgacgccg gcatgcgcga atgccgtgcc ccaggcggat ctcggcaaac ctgcggcgcg
    18181 gcgcagcgct tcgtccaggg gcgcccggcc gagccggatg cgcgtcgcga tctccacggc
    18241 cgctccgacg atcacccaca tgccgagccc gaaggcgagc ggggcgagcc agggaccgcg
    18301 tgtattgacg gcgaaagcgg tcgcagctgc cgcagccgcg atgatgagcg cggctatcag
    18361 cgtttggcct gccgctgcga gatcgccgcg cttccaggcg agcacgggcc cgaagggcag
    18421 ggcgatcagc agcggcacca tcagcggcac gaacgtcatg ttgaagaacg gcgggccgac
    18481 ggagatgcgc tggttggcga tcgcttcgag catcagagga tagagcgtgc cgagcagcac
    18541 cgtcgccgtc gcgacggtga gcaggatgtt gttgaggacg aggctgcctt cgcggctgac
    18601 cggtgcgaac gctccgccct ggcggagtgc gcccgccctc atggcgaaca gggtcagcgc
    18661 gccgccgatg aacacggcca tgatggcgag gatgaccatg ccgcgctggg ggtcgagcgc
    18721 gaaggaatgc actgaggtca ggatgccgga gcggacgata aaggcgccca tcagcgagag
    18781 cgagaaggtg aggatggcaa gcaggatcgt ccacaccttc agcgcctcgc gcttctccat
    18841 cacgacggcc gaatggagaa gcgccgtgcc ggcaagccag ggcatgaaag aggcgttctc
    18901 gacagggtcc caaaaccacc agccgcccca gccgagctcg taataggccc accaagagcc
    18961 ggtgccgatg ccgatcgtga ggaacatcca ggcggcgagc gtccaaggcc ggacccagcg
    19021 ggcccaggcg gcatccgtgc gcccctcgat cagggcggcg acggcgaacg agaaggccat
    19081 cgagaagccg acgtagccca tgtagaggaa cggcggatgg aaggcgagcg cggggtcctg
    19141 caggatcgg
//
LOCUS       DAORNH010000046        19113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2216_length_19113_cov_2.678350, whole genome shotgun sequence.
ACCESSION   DAORNH010000046 DAORNH010000000
VERSION     DAORNH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19113
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_19113_cov_2.678350"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK264_06870"
     CDS             complement(<1..251)
                     /locus_tag="PK264_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRD75642.1"
                     /translation="MTDGEYNTRLGSSASSSTVSNKAKTLCTNMKAQGITVYTIGFEL
                     DGNATAINVLQNCASTPDKFYNASSGVELRQAFRDIALRL"
     assembly_gap    262..274
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<275..1328)
                     /locus_tag="PK264_06875"
     CDS             complement(<275..1328)
                     /locus_tag="PK264_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD75643.1"
                     /translation="MSAILEDQAPQPAGSGEGGDLRSLITNFGRDKRGNIAMLFAMMI
                     TVAVSLVGGAVDYGRAIAARDKMQNALDSAVLAAARVWQTEGDINLAEAKAREFYKRM
                     KPTSIESDIVAFNPDVVNNKIKIEVEGMVHTPFLSLVRSAGYTISSSAEALLAVGGNS
                     EINLEISMMLDVTGSMSGQKIEDLKAAAKDLVDIVVWDDQSEYTSKVAIAPFAPRVNV
                     GSFASAFTGLPATKTFSGKSRKLITCVTDRVGTHEYTDVAPGAGAYVSAYKGDTGSSA
                     SSNSSNYSSSGSCSDPGEQIMPLTSNKTDLKARIDSFSASGATAGALGTAWAWYMISP
                     NWAHFWPEANRPVAYGT"
     gene            1689..3200
                     /gene="glpD"
                     /locus_tag="PK264_06880"
     CDS             1689..3200
                     /gene="glpD"
                     /locus_tag="PK264_06880"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRD75644.1"
                     /translation="MQRFDLLVIGGGINGVGIAADAAGRGLRVLLVEKGDLGGATSSS
                     SSKLIHGGLRYLEHYEFRLVRAALAEREVLMRRAPHIIWPLRFVLPHVTGLRPKWMIR
                     LGLALYDNLARRKAIPGSTGLDLAVDPAAGGLKPGLERGFAYWDCWVDDARLVILNAI
                     AAKAAGAEIATRTELMTARPDGGRWIATLAAPSGSRTIEAAAIVNATGPWVESVGRRI
                     AVARGVAGAIRNDAPHVRLVKGSHVVVPRFTGTDSAFLLQSSDGRVVFVLPFEDHFSL
                     IGTTDVPIDGDPGAATPSDAEVHYLLTLVGRFFRSPPTPADIVWRFAGVRPLVDDESA
                     NPSAASRDWRLELSPPGAGGPLLSVIGGKVTTFRRLAEAALERLAPHLPPMRPAWTAT
                     ATLPGGDIPSGGPASYAQRLAGSYPAIDARYLALLARRYGSRAHDLLGDASTPADLGE
                     RLGPALSEREVRFLVAEEWAATPDDILWRRTKAGLHVAPAERTDLADRVARLI"
     gene            3315..4292
                     /locus_tag="PK264_06885"
     CDS             3315..4292
                     /locus_tag="PK264_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HRD75645.1"
                     /translation="MAESGNGKLVTIFGGSGFVGRYAVRALARRGWRVRAAVRRPDLA
                     GHLQPMGGVGQIHAVQANLRYPESLARAVEGADAVVNLVAILSKSGKQTFDAVNTAGA
                     RTVAKAVREARCKSLVHVSAIGAARNAASNYARSKGAGEQAVLEEYPGAVILRPSLIF
                     GPEDDFFPRFAAMARMAPMLPLIGPKARFQPVFVGDVAEAIANTVEGKAAPGAVYELG
                     GPEVLTMRELLDRTQEWSGRNRPYLRLPFWLAKLGAALTAPLPNAMRPLTVDQVRLLQ
                     SDTIVSDAATADRRTLQGLGVAMPTAMSTVVPLYLEQFRPKGQFSHYRG"
     gene            4314..>4760
                     /locus_tag="PK264_06890"
     CDS             4314..>4760
                     /locus_tag="PK264_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD75646.1"
                     /translation="MTDETPAPPDDLKPHEFQAEVSRLLDLMIHSVYTEREIFMRELI
                     SNASDACDKLRYEAIARPELTAAEPRLAIEIRLDPAAGRLSIADNGIGMSRQELVDNL
                     GTIARSGTRAYLESISSPRKEGEPRAGSGLIGQFGVGFYSAFMVADE"
     assembly_gap    4761..4791
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4792..6237
                     /gene="htpG"
                     /locus_tag="PK264_06895"
     CDS             <4792..6237
                     /gene="htpG"
                     /locus_tag="PK264_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRD75647.1"
                     /translation="ANLWVSRGSGFKVGPAPAGRRPKGGRGTEVTLILKEEAKSFLEA
                     GEIERIVRTYSDHILFPVELVGADAPPRQINAASALWQRSRTEVTAEQYTEAFRAITG
                     EGGAPALTVHWRAEGRQSYAVMLFVPEHRPFDLFDPERKGRVKLYVRRVFITEEAGLL
                     PSWLRFVRGVVDSEDIPLNISREMLQNNPQLAQIRRALTSRIVSELESLAEKDGERYL
                     RIWETFGGVIKEGIYEDGERRDPLLKLLRVRSTAGPGWRSLADYVAALKPNQTAIYYL
                     TGDTLDRLKASPQLEAARARGVEVLLMADPVDSFWTALPIGFDGKPLVSLSRGEIDLS
                     AIPLVEPEKTAASGDEQKIAALIAACKTSLGDRVSDVKASKRLVDSAACLVAPAGGPD
                     LELERILGRANRGVGVKPILEINPGHALAKLAIAAQEARQSQRLHDLACLLFDQAMIL
                     DGQLPAEPGSFAQRINRLIGSAAPASDPTSA"
     gene            6350..>8011
                     /locus_tag="PK264_06900"
     CDS             6350..>8011
                     /locus_tag="PK264_06900"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRD75648.1"
                     /translation="MSAKPSPAEAASGQRAHSARKWVYAFGGGVSEGRADMKNLLGGK
                     GANLAEMASLGLPVPPGFTITTEVCTAYYDNGRKLPPDLPAAVDKALAEIGQKVGARF
                     GDPENPLLVSVRSGARASMPGMMDTILNLGLNGKTVEGLARRTGDARFAYDSYRRFIQ
                     MYSGVVLGLDHGEFEDILESHKNLNGLSADTDLKADDWKEVIEAYQATIKSETGKPFP
                     EDTGEQLWGAISAVFGSWQNARAITYRRLHDIPDDWGTAVNVQAMVFGNRGDTSATGV
                     AFTRNPSTGAKAIYGEFLINAQGEDVVAGIRTPQSLTEAARKEAGDKKPSLETAMPVV
                     FKELLGVFDRLERHYRDMQDIEFTIQEGRLYILQTRSGKRTTQAALKIAVELANEGLI
                     GRDEAVLRIDPGGLDQLLHPTIAPDAHKTILGNGLPASPGAAFGEIVFTADEAEAAKA
                     AGRQVVLVRTETSPEDIHGMHAAVGILTSRGGQTSHAAVVARGMGRPCVCGAGALRID
                     AKAGTMTAGTVVLKAGDLITIDGSTGEVLAGRVRMRQPELTGDFGT"
     assembly_gap    8012..8092
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8093..9118
                     /locus_tag="PK264_06905"
     CDS             <8093..9118
                     /locus_tag="PK264_06905"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948594.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRD75649.1"
                     /translation="RSLGAQGIGLCRTEHMFFDEDRILAVREMICAADEKGRRAALAK
                     ILPMQRADFEALFEIMAGLPVTIRLLDPPLHEFLPHEEKEQTAVAKSLGIAIETLRAR
                     IAELHEINPMLGFRGCRLAIRYPEITEMQARAIFEAAINAGRKSGTAVVPEVMIPLVA
                     WRREFDLLREVIDRTAAAVMKETGAKLAYQVGTMIELPRAALRADEIAAGDKGAEFFS
                     FGTNDLTQTCLGLSRDDAAPILADYANRGIFETDPFVAIDVSGVGELVEIGTTRGRKV
                     RSDLKIGICGEHGGEPRSVAFCHEAGLDYVSCSPFRVPVARLAAAHAAIREKRGETGR
                     QPNSSTA"
     gene            9133..10038
                     /locus_tag="PK264_06910"
     CDS             9133..10038
                     /locus_tag="PK264_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HRD75650.1"
                     /translation="MAAVLAVLLDIRQRLLRLLVGFVRLMPLDTAVRVSARSWRRLAP
                     LLSPKRHQRALENVAIAFPDMSEAERKRLVLAHWENLGRVMAETMQIDRLIAEPSRIE
                     VVSPVWQRYKEKLGAVIGVSLHMGNWELAVWPLTAAGAKPAAVYRTVKNPYVDRYLRH
                     IRRDLYPGGLLGKGKVDGSVEEGQRTARQIMDHVRHGGRLGLVCDLYDKFGIEVPFFG
                     KPAKSVTIAGMIARRIGARIWLARCVRVGDQSRFRIEIKELKVPRTANIGDDVRTITA
                     AMQAEFEAWIREVPEQWMWSNRRWG"
     gene            10147..10377
                     /locus_tag="PK264_06915"
     CDS             10147..10377
                     /locus_tag="PK264_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75651.1"
                     /translation="MSAKRRQKFATQIDAELLEAVRALARVERRHVHDLVEEALADLI
                     EKRRLGKPRENVMATYQSSLARYDRLYKKLAE"
     gene            10505..12049
                     /locus_tag="PK264_06920"
     CDS             10505..12049
                     /locus_tag="PK264_06920"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HRD75652.1"
                     /translation="MPFDPKAKDLVIGVVGTGTMGRGIVQVSAQGGMNVIAYDEKPGA
                     AEAARDFIAKLLMSQVEKGRLPEADAKAALGRITVASSLDDLAKANCVVEAIIERLDA
                     KQEMFARLDALCGPDTILATNTSSLLVSAIANRCKHPERVGGMHFFNPVPLMKLVEVI
                     PGLKTADWVTDAMMTIGRRMTREPILCKDSPSFLVNHVGRGMGPECQRILLENIGTAA
                     DIDRIMTGAPGFRMGPFTLADLVGIDIGIASQESAFAQFYGEPAFAPSPLTAVRAAGN
                     LLGQKTGQGWYAYGKDGKKVEPPMLATPAARPKSVWVHPSKSHQDLIDPLIAIFRASG
                     ATIESGEKPSDTALTVICPIGYDVTTACCDLKYDPRRTVAVNVLFGLKGPRELMVTPA
                     IDPAYREAAHGLVGSDGQPVIVINDSPGFVSQRAVAGMINVCCGIAQRGIGTPADIDK
                     GAKLGLGYPFGPIEWGQSIGPGRVLFILERLEAFYKEPRYKPSPWLKRRVMLGLPLAT
                     PEGKMA"
     gene            complement(12297..>13577)
                     /locus_tag="PK264_06925"
     CDS             complement(12297..>13577)
                     /locus_tag="PK264_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD75653.1"
                     /translation="LKSWLFGAEVYGPIGILLSQIFYCLPHAMLILTTALALADARLY
                     EAAETLGASKWRMFRTVTVPGIKYGLVSAVFVVFTLVITDFGIAKVIGGNFNVLATDA
                     YKQVIGQQNFEMGAVVGLVLLVPAVIAFAADRFIQRRQVALLTARAVPLEPKPRPLAD
                     WLALLFCLIVGGLVVFVLVVAIAASFITYWPYNLTPTLKWYNFSEYEPMGFMTYRNSL
                     VMGATAATLGTAIVFATAYLVEKGKGARPLRTLAQLIAMLPMAVPGLVLGLSYVFFFN
                     NTWNPFAVLYGSLFLLAINSIAHFYTTAHITATTALKQIDGEFEAVSASLKVPFWRTF
                     ARVTVPISLPAILDIWIYMFVNALTTVSAVIFIYGSSAKLASITMVHMEDGGATAAAA
                     ALATLIVATALAAKGMHVLLDTFVLARLQRWRKR"
     assembly_gap    13578..13671
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13672..13989)
                     /locus_tag="PK264_06930"
     CDS             complement(<13672..13989)
                     /locus_tag="PK264_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75654.1"
                     /translation="MARDTDTSRAAPAVDWENVVRVAALLAAIAFLLVAIALPLATLL
                     GRSVLDGDGNFVGLKNYITYVSTPTLVNSLWNSLWIALAATAIVVPIAFGYAYALRRT
                     CMPL"
     gene            complement(13995..15149)
                     /locus_tag="PK264_06935"
     CDS             complement(13995..15149)
                     /locus_tag="PK264_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HRD75655.1"
                     /translation="MHQTLDRIGGPQAGQLLQPSEPHLKIRNLTKTFGSFTALEDISL
                     DLQVGEFVCFLGPSGCGKTTLLRVIAGLDQQDAGTIEIAGRDVSRAPPKARDFGIVFQ
                     SYALFPNLTVRRNIAYGLVSRRLGRAEIHKRVTDLLALVGLPEQADKYPVQLSGGQQQ
                     RVALARALATEPSLLLLDEPLSALDARVRANLRSEIKQLHRRLGVTTIMVTHDQEEAL
                     AMADRIVVMDRGRVVQSGSPAEIYGRPATLFVADFVGATNLFDATVVSPGRVAVGALE
                     LHLAPGDGAAKISTGMRLKIGLRPEDVRVRHLAPDMANRVGVRIEMLEFLGPFARARL
                     TPLADPSLIIIADFSANALRDLALVEGQVIDIALPPEALRTFNATGSGTT"
     gene            complement(15228..16253)
                     /locus_tag="PK264_06940"
     CDS             complement(15228..16253)
                     /locus_tag="PK264_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD75656.1"
                     /translation="MLKRILALAMSAAAIVAMPMAPASAQKTKLTTYTALENDQLATF
                     KAEIEKAVPEVEIEWVRDSTGVITARILAEKAAPKADIVLGLAASSLLVFKKHGLIES
                     FAPKSADALKPMFKSADGSWTGMDAFLGVICYNTVEGQKAGLTAPKTWKDLLDPKYSG
                     KIVMPHPASSGTGYLMVAAWLQMMGEKAGWEYMDALHKNVGVYTHSGSAPCVQAAKGE
                     RIVGIGFDMRGAKEKTSGAPIDIVVPAEGTGWDMEASAIMKGTKKLDAAKKVMDWVAS
                     KAANELYSKYYAIVAHPAISNLPPNYPKDGEPRMFKMDLQIMADNRDAILAEWSKRYE
                     SKAAPKK"
     gene            complement(16424..17134)
                     /locus_tag="PK264_06945"
     CDS             complement(16424..17134)
                     /locus_tag="PK264_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HRD75657.1"
                     /translation="MATELARLAADDDIHTPGVGARIRNYFLTGLVVVGPVTITIYIA
                     WYFINVVDAWVKPYIPKIYNPESYVPFAIPGLGLLFAIIGLTLIGALAANLIGRSLIT
                     AGEVMLGRMPIVRNLYRAVKQIFESVVNATGPEQSFQKVAVMEFPSKGIWSLVIITGK
                     AAPQIAGVRPDDELISVFMPTHFVPPSGFLVFVPRSTVTPIDMTFEEAAKIILSAGLV
                     SQQTQSRLKDLAARKTGK"
     assembly_gap    17167..17213
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17648..18502)
                     /locus_tag="PK264_06950"
     CDS             complement(17648..18502)
                     /locus_tag="PK264_06950"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-class carbonic anhydrase"
                     /protein_id="HRD75658.1"
                     /translation="MRSGPVSLAVSAMFATAVGAASLLAAGEPPSSHEDQAAFCVSPQ
                     TPRDITKAHGLNFLMFESAPASSELNLCNLHFHANAEHRGPGFLKRASAGDKRGGFIC
                     NMTGSLSASELAMPAGSVCADHKTGKGLKTGDTIEVHWVHTSCPVKTEPGKGLKLCVS
                     CANPFLRVESQVFLLVNDPSATDFRKFDLASGTRDGYLHAKALPRGTGEPVTFRGSTT
                     GPKQTNKCDPTNVTWNVRPRCAKLDINSLGEWCKHNKYHENHAHGIRPLVTDLFSLSR
                     IYSSPHRY"
     gene            complement(18882..>19113)
                     /locus_tag="PK264_06955"
     CDS             complement(18882..>19113)
                     /locus_tag="PK264_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRD75659.1"
                     /translation="QVASYASRAAADQLIAKLKREHPALIGSRELIVNEAVVGAFGKQ
                     YRVWIGPYSAADAKPTCVKLRAAGLDCLPLGQ"
BASE COUNT         3301 a         6329 c         6075 g         3142 t
ORIGIN      
        1 aggcgcagtg cgatgtcacg gaaggcctgg cgaagctcga cgccgctgga agcattgtag
       61 aacttgtccg gggtcgacgc gcagttctgg agcacgttga tggccgtcgc attgccgtcg
      121 agctcgaagc cgatggtgta gaccgtgatg ccctgcgcct tcatgttggt gcacagcgtc
      181 ttggccttgt tggacaccgt cgaggagctg gccgaagatc cgaggcgggt gttgtactcg
      241 ccgtcagtca tgaggatagc gnnnnnnnnn nnnncggtgc cgtaagcgac gggccggttc
      301 gcctcgggcc agaagtgggc ccagttcggc gagatcatgt accaggccca tgccgtgccg
      361 agcgcacctg ccgtcgcacc gcttgccgag aagctgtcga tgcgtgcctt gaggtcggtc
      421 ttgttgctcg tcagcggcat gatctgctcg cccggatcgg aacagctgcc ggaagagctg
      481 tagttgctcg agttgctcga ggcgctcgat ccggtgtcac ccttgtaggc cgagacatag
      541 gccccggctc caggagcgac gtcggtgtac tcatgcgtac cgacgcgatc cgtcacgcag
      601 gtgatgagct tccgcgactt gccgctgaaa gttttggttg ccggcaggcc ggtgaaggcc
      661 gacgcgaagc tgccgacgtt gacgcgcggc gcgaacgggg cgattgccac cttcgaggtg
      721 tactcgctct ggtcgtccca tacgacgatg tcgacgaggt ccttggcggc cgccttcaga
      781 tcctcgatct tctggccgct catcgagccc gtgacgtcga gcatcatcga gatctcgaga
      841 ttgatctcgc tgttgccgcc aaccgccagg agcgcctctg cgctgctcga gatggtgtag
      901 ccggcgctgc gcacgaggct caggaacggg gtgtgcacca tgccctcgac ctcgatcttg
      961 atcttgttgt tgaccacgtc ggggttgaag gcgacgatgt cgctctcgat cgaggtcggc
     1021 ttcatgcgct tgtagaactc gcgcgccttc gcctcggcga gattgatgtc gccttcggtc
     1081 tgccagacgc gcgcggcggc gagcacggcg ctgtcgagcg cgttctgcat cttatcgcgg
     1141 gccgcgatcg cgcggccgta gtcgacggca ccgccgacga gcgagaccgc aacggtgatc
     1201 atcatcgcga acagcatcgc gatgttgccg cgcttgtcgc ggccgaagtt ggtgatcagc
     1261 gagcggagat cgcctccttc gccggagcct gcaggctgcg gggcctggtc ttcaagaata
     1321 gcggacacgg tacacctcct gtatgtgtgc catccgcact ataaaagacg aggccaatta
     1381 caaactcttt aaaatagcca gcaaccggac ggctatacac aatatataag caattttaat
     1441 aaaattgaat attgtttctc aatcgaatat tatgatacaa tacttccaga attcgatttt
     1501 atactactgg gacagcaaga atcgagccac caccaggcag caaatatgcg ggtttccgcg
     1561 ggccacatca ggaccacccg ccacttgacc tttgcggcaa ccaatcgttt accgacagtc
     1621 gcaattgtgc gatcggagcg cgaattcgcg ctgtctgatc gagattgagc tcgaggcggg
     1681 atcagcccat gcagcgcttt gaccttctcg tcatcggcgg tggcatcaac ggtgttggca
     1741 ttgccgcgga cgccgcagga cggggtcttc gcgtcctgct cgtcgagaag ggtgatctcg
     1801 gcggcgcaac ctcttcgtcg agctcaaagc tgatccatgg cggcttgcgc tacctcgagc
     1861 actatgaatt ccgcctcgtc cgggcagccc ttgccgagcg cgaggtgctg atgcggcggg
     1921 ctccgcacat catctggccg ctgcgcttcg tgctgccgca tgtcacgggc ctccggccca
     1981 aatggatgat ccggctcggt ctcgcgctct acgacaacct cgcgcgcaga aaggccattc
     2041 caggctcgac cggcctggac ctcgcggtgg accccgcggc tggcggcctg aaaccgggcc
     2101 tcgagcgggg cttcgcctac tgggactgct gggttgacga tgcccgcctc gtcatcctca
     2161 acgccattgc cgccaaggcc gccggcgccg agatcgcgac gcggaccgag ctcatgactg
     2221 cacgccccga cggcggacgc tggatagcca ccctcgccgc cccttcagga tcgcgcacca
     2281 tcgaggccgc cgcaattgtc aacgccaccg gaccgtgggt cgagagtgtc ggccgtcgca
     2341 ttgccgtggc gcgaggggtt gcgggcgcaa tccgcaacga cgcgccgcat gtccgacttg
     2401 tcaagggcag ccacgtcgtc gtcccgcgct tcaccggcac cgatagcgca ttcctcttgc
     2461 aaagctcgga tggacgcgtc gttttcgtac tccccttcga ggaccacttc agcctgatcg
     2521 gcacgactga cgtccccatc gatggcgatc cgggagccgc caccccgagc gacgcagagg
     2581 tgcattacct cctcactctc gtcggccgat tcttcaggtc gccgcccact cccgccgaca
     2641 tcgtctggcg ctttgccggc gtgcggcccc tcgtcgatga cgagagtgcc aatccgtccg
     2701 ccgcctcgcg ggactggcgc ctcgaactgt caccccctgg cgccggcggg cccctcctct
     2761 ccgtgattgg cggcaaggtg acgacgttcc gccgcctcgc agaggctgcc ctcgagcggc
     2821 tcgctcccca tctgccgccg atgcgccctg cctggaccgc cacggcgaca ctccccggcg
     2881 gcgacatccc gtccggtggc ccagccagct acgcgcaaag gctcgctggg tcataccccg
     2941 cgatcgatgc ccgctatctc gccctcctcg cgcgccgcta cggcagccgc gcacacgacc
     3001 tcctcggtga tgccagtacg cccgccgacc tcggggaacg cctcggccct gccctcagcg
     3061 aacgcgaggt ccgcttcctc gtcgccgagg agtgggcggc aacgcctgac gacattctct
     3121 ggcgccgcac caaggcgggc ctccacgtgg ccccggccga gcggacggac ctcgcggaca
     3181 gggtcgcccg actgatctga cagagccccg tgagctggca cttcgccgcg cttcacaggc
     3241 gcgctccgag ccattatgtc tcggtcgtct cgcggagatg cccgcgaagc tgacgtgaag
     3301 gggctcatac gaccatggct gagagcggca acggaaagct cgtcacgatc ttcggcggct
     3361 cggggttcgt cgggcgctat gcggtgcgcg cgctcgcgcg gcgcggctgg cgcgtcaggg
     3421 ccgcagtccg ccgcccggat ctcgcaggcc acctccagcc gatgggcggc gtcggccaga
     3481 tccacgccgt gcaagcgaac ctgcgctatc ccgaatcgct tgcgcgcgca gtcgaaggcg
     3541 ccgatgcggt ggtgaatctc gtcgcgatcc tctccaagtc ggggaagcag accttcgacg
     3601 ccgtcaacac ggccggcgcc cgcactgttg caaaggccgt gcgcgaggcc cgctgcaagt
     3661 ccctcgtcca tgtctcggcg atcggcgccg cacggaacgc ggcgagcaac tatgcgcgct
     3721 cgaagggggc cggcgagcaa gccgtgctcg aggagtatcc gggcgcggtg atcctgcgac
     3781 cctcgctgat cttcggaccc gaggacgact ttttcccccg cttcgccgcc atggcccgca
     3841 tggcgccgat gctgccgctg atcggcccga aagcccgctt ccagcccgtg ttcgtcggcg
     3901 atgtcgccga ggcgatcgcc aacaccgtcg aagggaaggc cgcgccgggc gcggtctacg
     3961 agctcggcgg acccgaagtg ctgaccatgc gcgagctcct ggatcgcacg caggagtggt
     4021 ccggccgcaa ccggccgtat ctgcggctgc ccttctggct cgccaagctc ggcgccgcgc
     4081 tgacagcccc gctgccgaac gcgatgcgcc ccctgaccgt cgaccaggtg cgcctcctcc
     4141 aatcagacac gatcgtgagc gatgccgcca ccgccgatcg ccgtacgctt cagggcctcg
     4201 gcgtcgccat gccgacggcc atgtcgactg tcgtgccgct ctacctcgag cagttccgcc
     4261 cgaaaggtca gttctcgcac tatcgcggct gatcgcttcc gccgagtacg cccatgaccg
     4321 atgagacacc cgccccgccg gacgacctca agccccacga gttccaggcc gaggtctcgc
     4381 gcctcctcga tctgatgatc cactcggtct acaccgagcg agagatcttc atgcgtgagc
     4441 tgatctcgaa cgcgtccgat gcctgcgaca agctccgcta cgaagcgatc gcccggcccg
     4501 agctcaccgc cgccgagccg cgactcgcga tcgagatccg cctcgatcct gcggccgggc
     4561 ggctctcgat cgccgacaac ggcatcggca tgtcgcgcca ggaactcgtc gacaacctcg
     4621 gcaccatcgc ccgctccggt acgcgcgcct atctcgagag catctcgtcg cccaggaagg
     4681 agggtgaacc gcgcgccggc agcggcctca ttggccagtt cggcgtcggc ttctattcgg
     4741 ctttcatggt cgctgacgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccaatctc
     4801 tgggtttcgc gaggctcggg cttcaaggtc gggcccgccc cggcaggacg ccggccgaag
     4861 ggcggtcgcg gcacggaggt gacactcatc ctcaaggaag aggccaaatc attcttggag
     4921 gcaggcgaga tcgagcggat cgtccgcacc tactccgacc acatcctctt ccccgtcgaa
     4981 ctcgtcggcg cggatgcccc ccctcgccag atcaacgctg caagcgcgct ctggcagcgc
     5041 tcccgcaccg aagtgacggc cgagcagtat acggaggcgt tccgcgccat cacgggcgaa
     5101 ggcggcgcgc cagcgctcac cgttcactgg cgcgccgagg ggcgccagtc gtacgcggtg
     5161 atgttgttcg tccccgagca ccgtcctttc gacctcttcg atccggagcg caagggccgc
     5221 gtcaaactct atgtccgccg agtcttcatc actgaggagg ccggcctgct gccgagctgg
     5281 ctgcgcttcg tgcgcggcgt cgtcgacagc gaggacatcc cgctcaacat cagccgcgag
     5341 atgctgcaga acaatccgca gctcgcccag atccgccgcg cgctgacgag ccgcatcgtc
     5401 tccgagctcg aaagcctcgc cgagaaggac ggcgagcgct acctgcgcat ctgggagacc
     5461 ttcggcggcg tcatcaagga aggcatctac gaggacggcg agcggcgcga cccgctgctg
     5521 aagctcctcc gcgtgcgctc gaccgccggc cccggctggc ggagccttgc cgactacgtg
     5581 gctgccctga agcccaatca gacggcgatc tactatctca ccggcgacac gctcgaccgc
     5641 ttgaaggcga gcccccaact cgaggcggcg cgggcgcgcg gcgtcgaggt gctgctgatg
     5701 gccgaccccg tcgacagctt ctggacggcg ctcccgatcg gcttcgacgg gaagcccctc
     5761 gtttcgctct cgcgtggcga gatcgatctc tcggcgatcc cgctcgtcga gccggagaaa
     5821 accgcggcga gcggcgacga gcagaagatc gcggccttga ttgcggcctg taagacctcg
     5881 ctcggcgatc gggtgagcga cgtgaaggcc tcgaagcgcc tcgtcgacag cgcggcctgc
     5941 ctcgtcgcac ctgccggtgg tcccgatctc gagctcgagc gcattctcgg gcgcgccaac
     6001 cgcggcgtcg gcgtgaagcc gatcctcgag atcaatccag gccatgcgct cgctaaactg
     6061 gcgatcgccg cacaggaggc gcgccagtcg cagcgcctcc atgatctcgc ctgcctcctc
     6121 ttcgatcagg ccatgattct cgacggccag ctgcccgccg agcctggctc gttcgcccag
     6181 cgaatcaatc gcctgatcgg ctcggccgcg ccggcaagcg atccgacgag tgcctgaggc
     6241 tctttccacc agaagatgct ggggcgaaag tacgctttgc aacggcgcga ccggccgggc
     6301 tacgactgca cgctgaagcc attcgtgaca gagcccctca ggagggccca tgagcgccaa
     6361 gccgtccccc gcagaagccg cctcgggcca gcgcgcgcac tccgcgcgga aatgggtgta
     6421 cgctttcgga ggcggggtct ccgaaggccg cgccgacatg aagaacctgc tcggcggcaa
     6481 gggcgcgaac ctcgccgaga tggcgagcct cggcctcccc gtcccgccgg gcttcacgat
     6541 cacgaccgag gtgtgcacgg cctactacga caatggccgc aagcttccgc ccgacctgcc
     6601 tgcggccgtc gacaaggcgc tcgccgagat cgggcagaag gtcggcgccc gtttcggcga
     6661 tcccgagaat ccgctgctcg tctcggtacg ctcgggcgcc cgcgcctcga tgccgggcat
     6721 gatggatacg atcctgaacc tcggcctcaa cgggaagacg gtcgaagggc tcgcgcggcg
     6781 gacgggagat gcccgcttcg cctacgacag ctaccgccgc ttcatccaga tgtactcggg
     6841 cgtggtgctc ggcctcgacc acggcgagtt cgaggacatc ctcgagagcc acaagaacct
     6901 gaatggcctc tccgccgaca ccgacctcaa ggcggacgac tggaaagagg tgatcgaggc
     6961 ctaccaggcc actatcaagt cggaaacggg gaagcccttt ccggaagaca cgggcgaaca
     7021 gctctggggc gccatttcgg ccgtgttcgg cagctggcag aacgcgcgcg ccatcaccta
     7081 tcgccgcctc cacgacatcc cggacgattg gggcacggcc gtcaacgtgc aggcgatggt
     7141 gttcggcaac cgcggcgaca cctccgcgac gggcgtcgcc ttcacgcgca acccttcgac
     7201 cggcgccaaa gcgatctatg gcgagttcct gatcaatgcc cagggcgagg acgtggtcgc
     7261 gggcatccgc acgccacaga gcctcaccga ggccgcccgc aaggaagctg gcgacaagaa
     7321 gccctcgctc gagaccgcga tgcccgtcgt cttcaaggag ctgctcggcg tgttcgaccg
     7381 cctcgagcgc cactaccgcg acatgcagga catcgagttc acgatccagg aaggccgcct
     7441 ctacatcctc cagacccgct cgggaaagcg caccacgcag gccgcgctga agatcgccgt
     7501 cgagctcgcc aacgagggcc tgatcggccg cgacgaggcc gtactgcgca tcgatccggg
     7561 cggcctcgac cagctcctcc acccgacgat cgcgccggac gcccacaaga ccatcctcgg
     7621 caacgggctt cccgcctcgc ccggcgccgc cttcggcgag atcgtcttca cggcggacga
     7681 ggcggaggcc gccaaggccg cgggccgcca ggtggtgctc gtgcgcaccg agacgagccc
     7741 cgaggacatc cacggcatgc acgccgcggt cggcatcctc acctcacgcg gcggccagac
     7801 gagccatgcg gccgtcgtcg cgcgcggcat ggggcgtccg tgcgtgtgcg gcgccggcgc
     7861 gctccgcatc gacgcgaagg ccggcacgat gactgccggc accgtcgtcc tcaaggctgg
     7921 cgacctcatc accatcgacg gctcgacggg tgaggtgctc gccggtcgcg tgcgcatgcg
     7981 ccaaccagag ctcaccggcg acttcggcac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcagcct
     8101 gggggcccag ggcatcggcc tctgccggac cgagcacatg ttcttcgacg aagatcgcat
     8161 cctcgccgtc cgcgagatga tctgcgccgc cgacgagaag ggccgccgag ctgcgctcgc
     8221 caagatcctc cccatgcagc gcgccgactt cgaggcgctg ttcgagatca tggcggggct
     8281 ccccgtcacg atacgcctcc tcgatccccc gctgcatgaa ttcctgccgc acgaggagaa
     8341 ggagcagacg gccgtcgcca agtcgctcgg gatcgccatc gagacgctgc gcgcgcgcat
     8401 tgccgagctg cacgagatca atccgatgct cggcttccgc ggctgcaggc tcgcgattcg
     8461 ctatcccgag atcaccgaga tgcaggcccg cgccatcttc gaggccgcga tcaacgccgg
     8521 ccgcaagtcc ggaaccgcgg tcgtgcccga ggtcatgatc ccactcgtcg cctggcggcg
     8581 cgagttcgat ctcctgcgcg aggtgatcga ccggaccgcc gccgccgtca tgaaggagac
     8641 gggagcgaag ctcgcctacc aggtcggcac catgatcgag ctgccgcggg cagcgctccg
     8701 cgccgatgag atagcggcgg gcgacaaggg ggccgagttc ttctccttcg gcaccaacga
     8761 tctcacgcaa acctgcctcg gcctctcgcg tgacgacgca gcgcccatcc tcgccgacta
     8821 tgctaaccgc ggcatcttcg agaccgaccc gttcgtcgca atcgacgtct cgggcgtcgg
     8881 cgagctggtc gagatcggca cgacgcgcgg ccgcaaggtg cgctccgacc tgaagatcgg
     8941 catctgcggc gagcatggcg gcgagccgcg atcggtcgcc ttctgccacg aggcggggct
     9001 cgactacgtc tcctgctcgc cgttccgcgt gccggtcgca cgcctcgcgg ccgcgcacgc
     9061 cgccatccgc gagaagcgcg gcgagactgg acggcagccc aactcgtcga cggcgtgagg
     9121 cggcccccgc cgatggccgc tgtcctcgcc gtcctcctcg atatccgcca gcggctcctg
     9181 cgcctgctcg tcggcttcgt gcggctgatg ccgctcgata ccgccgtccg cgtctcggcg
     9241 cgctcttggc ggcggctcgc gccgttgctg tcgccgaagc gccatcagcg cgccctcgag
     9301 aacgtcgcca tcgccttccc ggacatgagc gaggccgagc gcaaacgcct cgtgctcgcg
     9361 cactgggaaa acctcggccg cgtcatggcc gagaccatgc agatcgatcg cctgatcgcc
     9421 gaaccgagcc gcatcgaggt cgtgagcccc gtctggcaac gctacaagga gaagctcggc
     9481 gcagtaatcg gcgtcagcct gcacatgggc aactgggaac tggccgtctg gccgctgacc
     9541 gctgccggcg ccaagcccgc cgccgtctac cgaaccgtca agaaccccta tgtcgaccgc
     9601 tatctccgcc atattcgccg cgatctctat ccgggcggcc tcctcggcaa aggcaaggtc
     9661 gacggcagcg tcgaggaggg ccagcgtacc gcccgccaga tcatggacca cgtccgccac
     9721 ggcggcaggt tgggcctcgt ctgcgacctc tacgacaagt tcggcatcga ggtgccgttc
     9781 ttcgggaagc ccgcgaaaag tgtcacaatc gccggcatga tcgcgcgacg gatcggcgcc
     9841 cgcatctggc tcgcgcgctg cgtgcgcgtc ggcgaccagt cgcgcttcag gatcgagatc
     9901 aaggagctga aggtgccgcg cacggcgaac atcggcgacg acgtgcgcac catcacggcc
     9961 gcaatgcagg ccgagttcga ggcatggatc cgcgaggttc ccgagcagtg gatgtggtca
    10021 aaccggcggt gggggtgagc agagctcgat acttgagcga atgactgaca atggctcgag
    10081 acccacaacc agcctctgcc atgctgcgca actgacattt caagatttgc catgacgctt
    10141 ggggccatga gcgcaaagcg acgtcagaag ttcgcaacgc agattgacgc tgaactcctc
    10201 gaggccgtca gggccctcgc gagagtggag aggcgtcacg tccatgacct cgtcgaagaa
    10261 gcgctcgccg atctcatcga aaagcggcgg cttggtaaac cgcgcgaaaa tgtaatggcc
    10321 acctatcagt cgagcctcgc tcgctacgat cgcctgtaca agaagctcgc tgaatgacga
    10381 cggtgtacct ctccgtcgca ggagcgacgg gcggtacgcg cgcgccatca ccgccgctca
    10441 ttgcgtgccc tcgtgagccg acctaaggtc tcgccagaca gcagccatca tccgcgagcc
    10501 ccccatgccc ttcgatccta aggccaaaga tctcgtcatc ggtgtcgttg gcaccggcac
    10561 catggggcgc ggcatcgtcc aggtgtcggc gcaaggcggc atgaatgtga tcgcttacga
    10621 cgaaaagccg ggcgcggccg aggccgcccg ggatttcatc gccaagctgc tgatgagcca
    10681 ggtcgagaag ggccgccttc ccgaggccga cgccaaggcc gcgctcgggc gcatcacggt
    10741 cgcctcgagc ctcgatgatc tcgccaaggc gaactgcgtc gtcgaggcca tcatcgagcg
    10801 gctcgatgcc aagcaggaga tgttcgccag gctagacgca ctctgcgggc cggatacgat
    10861 cctcgcgacc aacacgtcct cgctccttgt ctccgcgatc gccaaccgct gcaagcaccc
    10921 cgagcgcgtc ggcggcatgc acttcttcaa ccccgtgccg ctgatgaagc tcgtcgaggt
    10981 gatcccgggc ctcaagaccg ccgactgggt cacggacgcc atgatgacga tcggccgccg
    11041 catgacgcgc gagccgatcc tgtgcaagga cagcccgagc ttcctcgtca atcacgtcgg
    11101 ccgcggcatg gggccggagt gccagcgcat cctgctcgag aacatcggga cggccgccga
    11161 tatcgatcgc atcatgacgg gcgcacccgg tttccgcatg gggcccttca cgctcgccga
    11221 ccttgtcggc atcgacatcg gcattgcctc acaggaatcg gccttcgccc agttctacgg
    11281 cgaaccagcc ttcgcgccat cgccgctgac ggcggtccgc gccgcgggga acctcctcgg
    11341 acagaagacc ggccaggggt ggtacgccta cggcaaggac gggaaaaaag tcgagccacc
    11401 aatgttggcg acgcccgccg cgcggccgaa atcggtctgg gtgcatccct cgaagtcgca
    11461 ccaggatctg atcgacccgt tgatcgccat cttcagggcg tcgggcgcga ccatcgagag
    11521 cggcgagaaa ccgagcgaca cggcactgac cgtgatctgc ccgatcggct acgacgtgac
    11581 caccgcatgc tgcgatctga aatacgaccc gcgccgcacc gtcgccgtca acgtgctgtt
    11641 tggcttgaaa ggcccgcgcg agctaatggt gacgccggcg atcgaccccg cctatcgcga
    11701 ggcggcacac ggcctcgtcg gctccgacgg gcagccggtc atagtcatca acgacagccc
    11761 aggcttcgtc tcgcagcgcg ccgtcgctgg catgatcaac gtctgctgcg gcatcgccca
    11821 gcgcggcatc ggcacgccgg ctgacatcga caagggcgcc aagctcggcc tcggctaccc
    11881 gttcggcccg atcgaatggg gccagagcat cggtccgggc cgcgtgctct tcatcctcga
    11941 gcggctcgag gccttctaca aggagccacg ctacaagccg tcgccctggc tgaagcgccg
    12001 cgtgatgctc gggctgccgc tggcgacacc ggaaggtaag atggcgtagt gaaggcccgg
    12061 cagatcgcaa agggggcgaa gaatacttcg ccggcctttg ccgcccgaca gatcgacaag
    12121 ctttccgctt gccgatggtc ttcacgccgc tcggcatgct cgccggcgcc gtgattggcg
    12181 gatggaaggg gtactcggga gcgtgagtgg ggttcgccct tcgggcgaaa tgtgaagggc
    12241 tgcctgccct tcacacaacc cggccagggc ctcggccctg gtccggagct gaatgatcag
    12301 cgcttccgcc agcgttgcag gcgggcgagc acgaacgtgt cgagcagcac gtgcatgccc
    12361 ttggccgcaa gcgccgtcgc aacgatgagc gtggcgagcg cggcggccgc cgccgtggca
    12421 ccgccatcct ccatgtgcac catggtgatg gacgcgagct tcgctgacga gccgtagatg
    12481 aagatcacgg ccgacaccgt cgtcagtgcg ttgacgaaca tgtaaatcca gatgtccagg
    12541 atcgccggca gcgatatcgg caccgtgacg cgcgcgaacg tgcgccagaa cggcaccttg
    12601 agggaggcgg ataccgcctc gaactcgccg tcgatctgct tcagggccgt cgtcgcagtg
    12661 atgtgcgccg tcgtgtagaa gtgcgcgatc gaattgatgg cgagcaggaa caggctgccg
    12721 tagagcacgg cgaacgggtt ccaggtattg ttgaagaaga acacgtagct gaggccgagt
    12781 acgagccccg gcaccgccat cggcagcatg gcaatgagtt gggcaagcgt gcgcagcggc
    12841 cgggcgccct tccccttttc gacgaggtac gccgtcgcaa acacgattgc ggtgccgagc
    12901 gtcgccgcgg tcgcgcccat gacgagcgag ttccgatagg tcatgaagcc catcggctcg
    12961 tactcgctga aattgtacca cttcagcgtc ggcgtcaggt tgtagggcca gtaggtgatg
    13021 aacgaggccg caatcgcaac gacaagcacg aacacgacca gcccgccgac aatgaggcag
    13081 aacaggagcg cgagccagtc cgcaagcggc cgcggcttcg gctcgagggg cacggcgcgc
    13141 gctgtgagca gagcgacctg tcggcgctgg atgaatcggt cggcggcgaa ggcgatcacc
    13201 gccggcacga gcagcacgag gccgacgacg gcacccatct cgaagttctg ctgcccgata
    13261 acctgcttgt aggcgtccgt cgcgagcacg ttgaagttgc cgccgatcac cttggcgatg
    13321 ccgaagtccg tgatcaccag cgtaaagacc acaaacaccg ccgagacgag cccgtacttg
    13381 atgccgggca ctgtcaccgt ccggaacatg cgccacttgg aggcgccgag cgtctcggcg
    13441 gcctcgtaga ggcgggcgtc cgcgagcgcg agcgcggtcg tcaggatcag catcgcatgc
    13501 ggcaggcagt agaatatctg agagagcagg ataccgatcg gcccgtagac ctcggcgccg
    13561 aagagccagg atttcagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagcggcat
    13681 gcaggtgcgg cgcagtgcat aggcatagcc gaaggcgatg gggacgacga ttgcggtcgc
    13741 ggcaagcgcg atccagaggc tgttccaaag cgagttcacg agcgtcggcg tcgagacgta
    13801 ggtgatgtag ttcttcaagc cgacgaagtt gccgtcgccg tcgagcacgc tgcgtccaag
    13861 cagcgtcgct aacggcagtg cgatcgcgac gagaaggaat gcgattgccg ccagcagggc
    13921 ggcgacacgg acgacattct cccaatcgac cgcgggggcg gctcgcgagg tgtcggtgtc
    13981 gcgggccatg gctctcatgt ggtaccggag cctgtagcgt tgaaggtccg cagtgcctcg
    14041 ggcgggagcg cgatatcgat cacctggccc tcgacgagcg cgaggtcgcg cagggcattg
    14101 gccgagaagt cggcgatgat gatgaggctc ggatcggcaa gaggcgtcag ccgggctcgc
    14161 gcaaagggac cgaggaactc gagcatctcg atgcgcacgc ccacgcggtt ggccatatcc
    14221 ggcgcaagat ggcgcacgcg cacgtcctcg ggtcggagcc cgatcttgag gcgcatgccg
    14281 gtgctgatct tggcggcccc gtcgccgggc gcgagatgca gctcgagcgc gccgacggcc
    14341 acccgacccg gcgacaccac cgttgcgtcg aagagattgg tcgctccgac aaagtcggca
    14401 acgaaaagtg ttgccggccg gccgtagatc tcggcgggcg acccggactg cacgacccgg
    14461 ccccgatcca tgacgacgat ccggtcggcc atggcgagcg cctcctcctg gtcgtgcgtc
    14521 accatgatgg tggtcacgcc gaggcgacgg tgcagctgct tgatctcgga gcgcagattg
    14581 gcgcgcacgc gggcatcgag cgccgagagc ggctcatcga ggagcaggag ggagggctcg
    14641 gtcgcgagcg cccgggcgag cgccacgcgc tgctgctggc cgccggagag ctggacggga
    14701 tacttgtcgg cctgctcggg gagcccgacg agggcgagaa gatcggtgac gcgcttgtgt
    14761 atctcggcac ggccgaggcg ccggctgacg aggccgtagg cgatattgcg gcggacagtc
    14821 aggttcggga agagcgcata agactggaag acgatgccga agtcgcgggc cttgggcggc
    14881 gctcgcgaca catcgcgacc cgcgatctca atagtgcccg cgtcctgctg gtcgaggcct
    14941 gcgatcacac gcagcagcgt cgtctttccg cagcccgacg ggccgaggaa acacacgaac
    15001 tcgccgactt ggagatcaag cgagatgtct tcgagggcgg tgaacgaccc gaatgtcttg
    15061 gtgagattgc ggattttcag gtgcggctcg ctgggctgca acagctgccc ggcctgaggc
    15121 ccgccgatcc gatccagcgt ctggtgcatt ccgcctcgcg cgcgtgacgg cttgtgcgcc
    15181 gacgaacggc ggggcgcaac agcgcgcccc gcggcataga gaaaatttca cttcttcggt
    15241 gcggccttgc tttcgtaccg cttcgaccac tccgcaagga tcgcgtcgcg attgtcggcc
    15301 atgatctgca ggtccatctt gaacatgcgc ggctcgccgt ccttcggata gtttggcggc
    15361 aggttggaga tcgcggggtg cgcgacgatg gcgtagtact tcgagtagag ctcgttcgcc
    15421 gccttgctcg ccacccagtc catgaccttc ttggcagcgt ccagcttctt ggtgcccttc
    15481 atgatcgccg aggcttccat gtcccagcct gtgccttcgg ctggcacgac gatgtcgatc
    15541 ggggcaccgc tggtcttttc cttggcgccg cgcatgtcga agccgatgcc gacgatccgc
    15601 tcacccttgg cggcctgcac gcagggcgcc gaacccgagt gcgtgtagac gccgacgttc
    15661 ttgtggagtg cgtccatata ctcccagccg gccttctcgc ccatcatctg tagccaggcg
    15721 gcaaccatca ggtagccggt gccggacgac gctggatgcg gcatcacgat cttgccgctg
    15781 tacttcggat cgagcaggtc cttccatgtc ttcggagcgg tgaggcccgc cttctggccc
    15841 tcgacggtat tgtagcagat cacgccgagg aaggcgtcca tgcccgtcca cgagccatca
    15901 gccgacttga acatcggctt cagggcgtcg gcgctcttcg gtgcgaagct ctcgatgagg
    15961 ccatgtttct tgaagacgag caggctcgag gcggcgaggc cgagcacgat gtcggccttg
    16021 ggcgctgcct tctcggcgag gatgcgcgcc gtgatgacgc cggtcgagtc gcggacccat
    16081 tcgatctcga cctcgggaac ggccttttcg atctcggcct tgaaggtcgc cagctgatcg
    16141 ttctcgaggg cggtgtaggt ggtgagcttg gtcttttgtg cactcgccgg agccatcggc
    16201 atggcaacaa tcgccgccgc cgacatggcg agcgccagaa ttcgcttgag cacgagtact
    16261 ctccccttga tggacacgga ctgcggaggc ttgacgaact gcaccggtcc tacgacagca
    16321 tcatgacagt taacgtcgat gctgtgcaac cccctccctc gccgccgcct ccctcgccac
    16381 tctggtgcgg cgatcgcccg gccgagcggc tgcaggccgt agctcacttg ccggtcttgc
    16441 gggcagcgag gtccttgagc cgcgactgcg tctgctggct gacgaggccc gcggacagga
    16501 tgatcttggc cgcctcctcg aaggtcatgt cgatcggcgt cacagtgctg cgcggcacga
    16561 acacgaggaa ccccgacggc ggcacgaagt gcgtcggcat gaagacgctg atgagttcgt
    16621 cgtccggccg cacgccggcg atctgcggcg cggccttgcc tgtgatgatg acgagcgacc
    16681 agatcccctt ggacgggaat tccatcaccg cgaccttctg gaacgactgc tcggggccgg
    16741 tcgcattgac gacgctctcg aagatctgct tcacggcgcg gtagaggttg cgcacgatcg
    16801 gcatgcggcc gagcatcacc tcaccagcag tgatcagcga gcggccgatg aggttcgccg
    16861 cgagtgcgcc gatcaaggtg agaccgatga tcgcaaacag cagcccgagg cccggaatgg
    16921 cgaagggcac gtagctctcg ggattgtaga ttttcgggat gtagggcttc acccaggcgt
    16981 cgacgacatt gatgaagtac caggcgatgt agatcgtgat ggtgacgggg ccgacgacca
    17041 cgaggccggt caaaaaatag ttgcggatgc gggcgccgac gccgggtgtg tggatgtcgt
    17101 catcggcagc caggcgggca agctcggtcg cgatctccgt gtgatccgtc gcgccggggc
    17161 tcggttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgtcgag
    17221 ccaggcgagg atgcgggcgc ggtcgccgtc gaggtccgga accgcgctgc gcgtgccacc
    17281 cgccctgcac gatcgacgct tgtgccacaa caatcccgca accccaagcg aagtcgtcta
    17341 gcaactttcg tcgatctgga cgccgctcga attccggctc cgacatcttg ttccagcccg
    17401 ggaccggtag caagcacatc tcggtgccgt aaggaaaacc gactactcga tgtgtccagc
    17461 ctagctctgg cacctgatgt atagatctta gccaagattc actcggggac acggcgtcgg
    17521 tcgccacagt gagcaggcct cactgaccag gccaatgcaa atgccagcct cgatggcccg
    17581 cggaccatcg aggcgggacg gttgcgaatc gagcgagatg cggctcttca gagtgatgcc
    17641 gtcgaggtca gtatctgtgc ggcgatgaat agatccggga cagcgagaac aggtcggtca
    17701 ccaagggccg aataccgtga gcgtggttct cgtggtattt gttgtgcttg caccattcgc
    17761 cgagcgaatt gatgtcgagc ttggcgcagc gcggacgaac gttccaggtg acattggttg
    17821 gatcgcattt gttggtctgc ttaggaccgg tcgtggagcc gcgaaacgtc acgggctcgc
    17881 ctgttcctcg cggcaaggcc ttcgcatgca agtatccatc ccttgttccg gacgcgagat
    17941 cgaatttgcg gaaatcggtc gccgaggggt cattgacgag cagaaatacc tggctctcga
    18001 ctcgcaaaaa cgggttcgcg catgacacac acagcttcaa gcccttgccc ggctctgtct
    18061 tgacgggaca cgacgtatgc acccagtgca cttcgatggt gtctcccgtc ttaagaccct
    18121 tgccggtctt atgatccgca caaactgaac cagccggcat tgcaagttcg ctcgccgaga
    18181 gagagcccgt catgttgcat ataaacccac cccgcttatc cccggcggac gcacgcttca
    18241 ggaaccctgg cccgcgatgc tccgcgttcg catggaaatg caaattgcat agattgagct
    18301 cactggaagc aggcgcgctc tcgaacatca ggaaattgag accgtgggcc ttcgtgatgt
    18361 ccctcggcgt ttgcggcgag acgcagaatg ccgcttgatc ctcgtgggat gagggaggct
    18421 cgcccgccgc taacagcgat gctgctccca ccgccgtcgc gaacattgcc gaaacggcta
    18481 aagaaaccgg accactacgc atcaccaccc ccgctacact gtctacccaa ctttgacctc
    18541 gcgttgccct cgcaacgcgc ggatacacta ttggatccgg cagtgctgag tgtaacccca
    18601 accgccacaa tcgccggcgc aattctgcct tcaaaggcag ctcggtgccg cactgcggct
    18661 cagggtcacg tccggggccc tataccggtg ttgaatcggc cgcctattct cgaagcggga
    18721 gcgtctttga cggcccagtg gtcgcgatgt ttaaataacg cgaatgccaa aacccccctt
    18781 gcaagttggg cggtactggc ctgcttcaca gctgagaggg aagcggtcac gcgaagtcgg
    18841 cagcgcaaaa tcttctcgcg gtgtcccgcc gcgtggggcc ctcactgccc gagtggcagg
    18901 cagtcgagcc cggccgcacg gagcttcacg catgtgggct tcgcgtcggc cgccgagtag
    18961 gggccgatcc acacgcggta ctgcttgccg aaggcgccga cgaccgcctc gttgacgatc
    19021 agttcgcggc tgccgatgag cgccgggtgc tcgcgcttca gtttggcgat cagctggtct
    19081 gcggcggccc gagatgcata ggaggcgacc tgg
//
LOCUS       DAORNH010000047        18850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2245_length_18850_cov_2.728545, whole genome shotgun sequence.
ACCESSION   DAORNH010000047 DAORNH010000000
VERSION     DAORNH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18850
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2245_length_18850_cov_2.728545"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1365
                     /locus_tag="PK264_06960"
     CDS             139..1365
                     /locus_tag="PK264_06960"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75660.1"
                     /translation="MTSREPQSGTSHWSRSQRKVLALAFATAVVGWGFSVYGPAVLLG
                     MLKDTAGWSLAGISAAISLHYLVGALLVMWLPEVHARIGLVSSIRIGIVAATIGLIAW
                     SAAWSVYAMLPAAVLTGVGWALLGAATVNALVAPSFDRDRPKALALAFNGATLAGVIF
                     TPLLVAANSALGQIRGYAIVALTASIVLLGLVVMLGSDLSSRDPTHSPPSMPPRLTIG
                     ELMSERRFVSLAIATCLGVFAQIGILTHLFARLVQPLGATGASLAVSMASIGVVVGRS
                     LFAEAFQRSDRRLVGVAGFLLQIPGALALAFGSSTPVLAAGALVLGLAAGPVSSLGAV
                     VAQSEFEARDVPRVVALVTSINQIGLAGAPLAVGLIAEVAGGYLTSYCALAAVMALGA
                     LSLLIGRESKACSGAA"
     gene            complement(1351..1788)
                     /locus_tag="PK264_06965"
     CDS             complement(1351..1788)
                     /locus_tag="PK264_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD75661.1"
                     /translation="MPAADSPSRRGLDGRSQANALDIYVGHRLAFRRAIIEMDRAELA
                     NRIGLSIATIESMEAGAQRIGVSDLYRLAEILEVPVAWFYEGLPQGPSPAASTFVRDT
                     ETSLTGASEDELSLIQSYRKLLPEERSRLLRYSRRLRRRSGGT"
     gene            2101..2877
                     /locus_tag="PK264_06970"
     CDS             2101..2877
                     /locus_tag="PK264_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75662.1"
                     /translation="MDRSVSFERVPLLRGIDIDHGPREQIGQLLLTATDAAAARGLKL
                     YLTTLADILAINEANQDSWGPLAPMYNPRLWGPDDGEAMGIVGLNADGVAVATQAVRL
                     FELTETSFADEAKTMRLFYRDPEAQSRPGESCTVTAPSAGSIKGRVAMSGGVWYRPDY
                     RGKQLANILPRIGRACSLAQWDIDYSVAINSEAVFKGGMAGRAGFPHSEWELLLHFAP
                     IGDIRFAINWITKPELIDDFSTFVAGFDVEGKRAVENRRA"
     gene            complement(2815..>3381)
                     /locus_tag="PK264_06975"
     CDS             complement(2815..>3381)
                     /locus_tag="PK264_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75663.1"
                     /translation="HLDLIAASFQQIRTAQDRLARHRLRRRITPADLGPSHPLRSLIE
                     RWREVGAVWDRERLDGIIRSALDDRYVLASPSTTGNQVTICDVGSGYGLFNGSWLSRC
                     RGLSMSDMPDYRYGRWVADAHIEAAKDEEPLLEHVDVAICWPRQEPRRHVYQRVILPV
                     GTGKDRRLLSASILDSALAFDVEAGDER"
     assembly_gap    3382..3395
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3396..3696)
                     /locus_tag="PK264_06980"
     CDS             complement(<3396..3696)
                     /locus_tag="PK264_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75664.1"
                     /translation="MRELQLGTLLFDTSGQPWDSESPILRQRWGGIHAGGDFTDFILR
                     NIGFAEVRGIGDDGVELRFRPTRASELALVAAGSWISDRPFRRFAMTTYNGTWHPR"
     gene            4047..4439
                     /locus_tag="PK264_06985"
     CDS             4047..4439
                     /locus_tag="PK264_06985"
                     /inference="COORDINATES: protein motif:HMM:NF039385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HRD75665.1"
                     /translation="MFRAHRQWHVSPTTVTAAGRSIPRADIVAIDVELLEARDTGGAK
                     LLAIAYMLATAAILIGVFEFGFSQRYLLAAGLCGMIGLSGLQDAWQATTARTYEVSIR
                     LASGETARVTLADELEARGLMAAISAPA"
     gene            4567..4641
                     /locus_tag="PK264_06990"
     tRNA            4567..4641
                     /locus_tag="PK264_06990"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4599..4601,aa:Thr,seq:ggt)
     gene            complement(4675..5469)
                     /locus_tag="PK264_06995"
     CDS             complement(4675..5469)
                     /locus_tag="PK264_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75666.1"
                     /translation="MIQATYAVEAYNLSQASENKIHDDSVARRFGFEGGLVPGVEVYA
                     YATHLAVARWGRSWLEGGSMSIRFLKPVYDGLTAIVTALDVPGEEAVALHVACNGTLS
                     ATARADIRAPATDARGHLDGAAIASPLVDRPPADETSLAEGRLLRSPPQAIGAAELAD
                     YLAAVREQHDLYERDGIVHPGQILRVCNLVLKENVLLPPWVHTGSQVQNLSLARVGET
                     LEGRARIERNYKKKGHHLVDLDVLVTADTRPVARVLHTAIYRLRGG"
     gene            complement(5560..7065)
                     /locus_tag="PK264_07000"
     CDS             complement(5560..7065)
                     /locus_tag="PK264_07000"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRD75667.1"
                     /translation="MKPRQPVLDRRKFLGSSALASGALLFGPGRAEAQGAPPVLRIET
                     RTIEVNKKAAKVFHVAGPGGRNGILATEGDRFNGTVVNASHEPLQLHWHGQVKAPFDQ
                     DRARPGGGSLPIGGSDTFDFELTPGTHWMHSHTLSEQLLLAAPMIAREKNAGDVQDVV
                     VMLHDFSFRSPEEILAGLGGAGVHSSHDAAPSKGAPVASEGSRSGSGAGSRTGSRSAA
                     TGAPKGSAAHGHRPGMVHANDVTYDAYLANERTFDDPEVVRVAKGARVRLRIINGATA
                     TAFFITTPGMKSACIAVDGNACAPISAASYPLAQGQRVDLVVTIPNSGGAFPVIAGVE
                     GVRFQTGIILATVGASITRRSTSALAARPLIDTAFEALVRASAPLTARAADKAFNLTL
                     GEGDNYRWTINNRIHGEHEPLKVGRGERVEISFANPTGMMHPMHLHGHHFQVVAIGGR
                     RFAGAMRDTVIVPPNLPVTIALDAEHKGQWFLHCHHLYHMATGMMTELHVA"
     gene            complement(7180..8736)
                     /locus_tag="PK264_07005"
     CDS             complement(7180..8736)
                     /locus_tag="PK264_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRD75668.1"
                     /translation="MTDVSRREALTLAAAASLGAGLPLGVGAEPAAAQSFGAAAAGPR
                     PTLTLLLVNDIYKMSGVDGRGGFARLAAIARAERAKGVPLLYAHAGDMFSPSLMSGFD
                     QGAHTVELLNVVPPDVFVPGNHEFDFGPDVYMRRRGESRFPYLAANLRGADGKPLQGH
                     DDNRLLEAGPIKVGVFGVALATTPFMSQSGPLKFLDEMETVRTQAKLLRAAGADLVVA
                     VTHTDFERDLEIMRSKLVDVLLTGHDHDLRLAYDNRVVMVESGEEGEYVTAIDIFAAV
                     SQQDGRRKVTWRPRFRVIDSALVAPDPAAQKIVDRLEAELGRELDVAIGKAGVELDTR
                     SATVRSQEAVFANIVADAIRLSTGADIGLTNGGALRGNRLYPAGHVITRRDVLSEMPF
                     GNRTVMVEITGRDLKDALENGVSDLDNRGGRFPHVSGLTIVVNEKAKSGERVVAIERD
                     GKPIDPAGKYKVASNDFMLAGGDGYSALARGRTLIGKTDGKLMASVVMSHIRSLGTIG
                     ARIEGRIVLR"
     gene            8881..9519
                     /locus_tag="PK264_07010"
     CDS             8881..9519
                     /locus_tag="PK264_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD75669.1"
                     /translation="MPGLSGLAPDLRQAIAAAGRVLRLPKGSRIFGPGQAPQNYLLLL
                     EGDIRVSQVGESGREIVLYRVLAGESCALTTGCLLGGGDYTAEATADTDVEAVAIPRT
                     LFDDLIARSAEFRRFVISAFSQRVTDLFKLVEEIAFQRIDVRLAHKLIELAGAGDEVA
                     MTHQQIAAELGTAREVVSRHVHELQRRGTVVSNRGNIRIADRSALERLAGGS"
     gene            9622..9822
                     /locus_tag="PK264_07015"
     CDS             9622..9822
                     /locus_tag="PK264_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRD75670.1"
                     /translation="MTNVGSIDRILRVIVGAGLISLVFFGPQTNWGWIGVVPLLTSLI
                     GYCPAYTLLGVSTCPLDRSKRG"
     gene            9868..10410
                     /locus_tag="PK264_07020"
     CDS             9868..10410
                     /locus_tag="PK264_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRD75671.1"
                     /translation="MPPTIETTPMTLLTQKAWSPYLAGAVIGLLQIPTFILMDTALGA
                     SSSFVTAAAHILSFVDAGSTQVKYFASHMWGAKNWWQVAVVIGIALGAFISMRLSGAN
                     RQTISPVWNRTTGTTSLAARAPIAFLGGFIMLFGARIAGGCTSGHGVSGMAQLAVGST
                     LAVAAMFAGGIITAMLLRRS"
     gene            10427..11029
                     /locus_tag="PK264_07025"
     CDS             10427..11029
                     /locus_tag="PK264_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HRD75672.1"
                     /translation="MTVLSGILIGLATGVVFGFALEKSRVFEPGVIVGQMQLRSTLML
                     KVFLAAVATGLLVLAVMNGVYGVKLSLKPLLWRADIIGGLILGVGITISGACPGTSLA
                     QLGAGYRDAFFVVLGGIAGAIAYGYNDAAITKLFAESGQKLGFDKLLGMPFWLTAVIL
                     AAVLIVVLVALEIMLPWRSEVGDDHDGVTDRHAGRLVAAE"
     gene            complement(11044..13347)
                     /locus_tag="PK264_07030"
     CDS             complement(11044..13347)
                     /locus_tag="PK264_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRD75673.1"
                     /translation="MTTNEAKRVRREDERMVTGRGRYVADIMMPDMAYGVVVRSSLPH
                     AELRGIDTAAAKAAPGVLAVLTAADLAADGITEISPVVEQKRPDGSKAPEARRPVLAR
                     DRVRFVGEPIAFVVATSLRAAQDAAELVVPDYGDLPAVVTTAQARAPGAPAIWPAAPD
                     NVALYWTRGDLGAADKVIAGAKHVARLKSHVTRVNANSIEPRGALGYVDSAGRLVLHV
                     SNQGPHGLKGSLAPLLKVTPDKLRIVVGDVGGSFGMKSGVYNEDVLVLAAARRLGRPV
                     RWISDRLEGFLSDEHGRDVGIEATLALDGEGQFLALKINCEINLGCFLTGRSMGLLNN
                     IGGISGVYRTPIVAAEITGVLTNTQVTAPYRGAGRPEATYAVERVIDIAARQMGIDPF
                     ELRWRNLVQPESMPYATGFIFTYDCGNFPRNMTEAARLADRSGFEARRADAKSRGKLL
                     GLGIANPVEVAGGPFLKPGKDAAMLSIAGDGTVTVTAGALSTGQGIETTFVELAASKL
                     GLPASQITYRQGDTDAIGHGRGSGGSSALCVGGTATLQALDAVIEKAKVVASDMLETA
                     AGDLRFADGRFTVVGTDRSVALGDVARRAADKGEPLAAGAEFQPTKVTFPNGCHLCEV
                     EVDPVTGETEIKRYTVVEDVGRVMNPTLMKGQIHGGVAQGVGQALFERMSYDPESGQL
                     MTASFMDYTMPRADDLSAITVGTNEVPTAVNPLGAKGVGEAGTVGSMVAAVNAVCDAL
                     APLGVNHIEMPLTPDRVWVAIQGATLA"
     gene            13531..14166
                     /locus_tag="PK264_07035"
     CDS             13531..14166
                     /locus_tag="PK264_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRD75674.1"
                     /translation="MIDFYTWSTPNGRKVSIMLEEIGLPYTTHAVDINKDEQFKPEFL
                     KISPNNRIPAIVDRDNGLMLFESGAILMYLAEKTGKLVPKDEKGRWHAVQWLMWQMGG
                     VGPMLGQVHHFVKYNAGKAPYAEERFLKEGKRLYGVLDRRLAEAPYVAGDYSYADIAI
                     WPWISRFEWQTIDMKAFPNVMRWYKAIAARPAVQRGYDVPVKQPGIPMPAA"
     gene            14376..15302
                     /locus_tag="PK264_07040"
     CDS             14376..15302
                     /locus_tag="PK264_07040"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRD75675.1"
                     /translation="MKTSRIFSGVIAPASTPFGEDGMPDEDRFIEHCHWLLEDGCTAL
                     APFGTTSEANSLGLDERMELLEALVESDIDPAKLMPGTGTCSVTDTINLTDHALDLGC
                     GGVLMLPPFYYKNPSEDGLFRFFADVIEEISDDDLKVYLYHIPPIAQVGFSLPLIERL
                     IKAYPKTIAGLKDSSGDWSNTKAIIEAFPMLEVFPGSEAFLLDGLKIGGAGCISASAN
                     VAAKKLRQLFDGWQGPDAARLHEEILGLRKTIQSYPMIPLLKAIIGHYRQDPGWSAVR
                     PPFTPMSAADTAAAVKKLADTHGFKLDFADAA"
     gene            15925..16785
                     /locus_tag="PK264_07045"
     CDS             15925..16785
                     /locus_tag="PK264_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD75676.1"
                     /translation="MADRSITLEGGPLGFLLIHGLGGTPAELRLVAQALNRAGHTVHC
                     CQLAGHCATVNDLRLSVWQEWYASVEAAHDRLSASCKTIVAGGLSMGGLLAIHLAHQR
                     PTGVQGLAIYAPTLVLDGWSVPKLWSYILRWVRPIPGFKSGFMVKERPPYGLKDERIR
                     AIVLQHMQSGDAGVAGVFETPMHSMAQFNAFSAVVRRELPSVRQRALILHPRDDDMAS
                     IENSHYLQRNLGGIVELVVLNDSYHMITLDRQRQVVIDHTLALASRLAKLGPEGGAAR
                     LVPAARGAAE"
     gene            16782..17945
                     /locus_tag="PK264_07050"
     CDS             16782..17945
                     /locus_tag="PK264_07050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75677.1"
                     /translation="MSGEGELVCRCHASVGEIDRMEWDRLFPGDPEGHTYYTACEAAP
                     PPAFTLGAVTVRRGGRLLAAAPTFRLIYRLDTPLQDRMRGLGDWLHRRLPRLINLPIL
                     CLGSPLADRCHLGLDPALTPGERDAVMAALLTGLDAEAARHHARVVAVKDLTGRDGET
                     LGAPFKREGFTRIASLPVAVLELPFADEGAYLASLSSATRKDIRRKLKSSGDLRIETR
                     SSIAGLEAEIASLYEETRRQSGVDYGEFEGLAPSYFPAVSVHLGERAQWMLYWLGDRL
                     IAFNLLLVGDERVIDKFIGMRYPEAREHNLYAVSWMANVRFCLARGIRRLQTGQTAYS
                     SKVRFGSALEPMLVFARHRIGVVNALFKMFGPRFAFDKYDPELKALAARKGEA"
     gene            complement(17952..>18850)
                     /locus_tag="PK264_07055"
     CDS             complement(17952..>18850)
                     /locus_tag="PK264_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD75678.1"
                     /translation="GRRLILALAEKADVLIQNLKPGALAKLGLAVGELRRRNPRLITC
                     SISGYGERGPYADRKAYDLLVQAEAGLAAVTGGPEAPSRVGISIVDIATGLHAYEAIL
                     EALLRRGRTGDGAEIELSMFDCVAELMGVPIMYGAAGKPPARIGLAHPSLAPYGVFMT
                     RDAVPILISIQNEREWKSLAERVLERPDAVTDPRFSSSSARVANRAATDTLVADWFGA
                     RDHEPVIAALSRADIAFGRVNDVPALLEHPHLRMMDVVTPSGPTRVPALPALWDGVTD
                     PPSATPALGADTAAVTAEFLKG"
BASE COUNT         3036 a         6001 c         6438 g         3361 t
ORIGIN      
        1 tgcgcagaag taggccttgc aaagacgatc cttgccaacg atgtgtgtgc gtggataagt
       61 gatagctcgc tatttcggtt gtgcactttt tcctcagtct gttgtcgcag ttgtgcgtcg
      121 attcgtgagg gtgggcgttt gacgagccgt gagccgcaga gtgggacttc gcattggagc
      181 cggagccagc ggaaagtcct ggcactcgcc ttcgccacgg cggttgtcgg gtggggcttc
      241 tctgtctacg gacctgccgt cctcctcgga atgctcaagg acacggccgg ctggagcctc
      301 gccggcatct ccgccgccat ctcgctgcac tatctcgtcg gcgcccttct ggtgatgtgg
      361 ctgcccgagg tgcatgcaag gattggcctc gtatcgtcga tccgaatcgg cattgtcgcc
      421 gccacgattg ggctcatcgc atggagcgct gcgtggtctg tctatgccat gctgccggcc
      481 gctgtcctga caggggtcgg ttgggcgcta ctcggtgccg caacggtcaa cgcgcttgtc
      541 gcgccctcct ttgatcgcga ccgccccaag gccctggccc ttgccttcaa cggggcgacg
      601 ctggcaggcg tcatcttcac cccgctgctt gttgccgcga actcggcgct cggccagatt
      661 cgcgggtatg ccatcgtcgc gctcaccgcc tccatcgtgc tgctcggcct ggtggtgatg
      721 ctcggctccg acctttcttc gcgcgatcct acgcactcac ccccgtcgat gccgccacgc
      781 ctcaccatcg gcgaactcat gtcggagcgg cgcttcgtga gcctggcgat tgccacctgc
      841 ctcggtgtct tcgcccagat cggcatcctg acgcacctct tcgcgcgcct cgtccagccg
      901 ctcggggcga cgggggcgag cctggcggtg tcaatggctt cgatcggcgt ggtagtcggg
      961 cggtcgctgt tcgccgaggc tttccaacgc tctgaccgcc ggctcgtcgg cgtcgccgga
     1021 tttctcctgc agatccctgg tgcgctggcg ctcgccttcg ggtcgagcac tccggtgctg
     1081 gcagccgggg cgctcgtgct cggccttgcc gccggaccgg tctcctcgct cggtgcggtc
     1141 gtagcccaga gcgagttcga ggcgcgtgat gtgccccgcg tggtggccct ggtcacttcg
     1201 atcaatcaga tcggacttgc gggggcaccg ctggcggtcg gactgatcgc ggaggtcgcc
     1261 ggcggctacc tgacctccta ctgcgcgttg gccgcggtca tggccctcgg cgcgctgtct
     1321 ctgctgatcg gtcgcgaatc caaggcgtgc tcaggtgccg cctgagcgcc gtctcaggcg
     1381 ccgcgagtag cgcagcagcc gggaccgctc ctcgggtagg agcttgcgat aggactggat
     1441 gagggaaagc tcgtcctcgc tcgcccctgt gagcgacgtc tcggtatcgc gcacgaatgt
     1501 cgatgctgcg ggtgaagggc cttgcggcag gccctcatag aaccaggcga ccggaacctc
     1561 gagaatttcg gcaagacgat aaagatcgga aacgccgatc cgttgggcgc cggcttccat
     1621 actctctatg gttgcgatcg aaagtccgat gcgattcgca agttcggcac gatccatttc
     1681 gatgatggcg cgccggaagg cgagacggtg gccgacataa atgtcgagcg cattagcttg
     1741 ggagcgtccg tccaggcctc ggcggcttgg gctatcggcg gcgggcatcg agtccctttc
     1801 agaagagagg cgtcaaccgc cggaaggtcc aggcctgggc gagtttattc aaatcctatg
     1861 ccgatgcgaa cggtccgcgt gcgcattgtg gcgaattcgc aacattcaat gagctgaagc
     1921 gacgccggct gacctcacat gctgctcctt tagttcgcgc agtgaaacgt aaacaaacca
     1981 acttgcggtc gctgcaactc agtgaattgc ggggacagcc tgtgggtaac agtcggcgtg
     2041 cggattgtct atccgcgcgt tgcgatacgg tgttttggca accttaagtg gagttttcac
     2101 atggatcgct cggtaagctt cgagcgggtg ccgctgcttc gcggaattga tatcgaccac
     2161 ggcccacgcg aacagatcgg tcaattgctt ctgacggcga ccgatgccgc tgcggcgcgc
     2221 gggctgaaac tctatctgac cacacttgcc gacatccttg ccataaacga ggccaaccag
     2281 gacagctggg ggccgctcgc gccgatgtat aatccacgcc tgtgggggcc ggacgatggc
     2341 gaggcgatgg ggattgtggg gctgaacgcg gacggtgtcg ccgtggcgac gcaggctgtc
     2401 cgcctgttcg aactcaccga gacatccttt gcggacgagg ccaagacgat gcggctcttc
     2461 taccgtgatc ccgaagcgca gtcgagaccg ggagaaagct gcacggtgac ggcgccgtcg
     2521 gccggatcga tcaagggccg tgtcgcgatg tcggggggtg tctggtaccg cccggactat
     2581 cggggcaagc agcttgccaa catcctgccg cggatcgggc gcgcatgctc gctcgcgcag
     2641 tgggacatcg actactccgt cgccatcaac tccgaggctg tcttcaaggg cggcatggcg
     2701 ggtcgcgccg ggtttccaca ctcggagtgg gaactgctgc tgcacttcgc gcccatcggc
     2761 gacatcaggt ttgcgatcaa ctggatcacc aagccggagc tgatcgacga cttctcaacg
     2821 ttcgtcgccg gcttcgacgt cgaaggcaag cgcgctgtcg agaatcgacg cgcttagcag
     2881 gcggcggtcc ttgcccgtgc cgactggcag gatgacgcgt tggtagacat gccgacgcgg
     2941 ctcctgccgg ggccagcaga tggcaacgtc gacgtgctcg aggagcggct cttcgtcctt
     3001 tgcggcctcg atatgggcgt ccgccaccca gcgaccatag cgatagtcgg gcatgtcgct
     3061 catcgagagg ccgcggcagc gcgacagcca cgagccgttg aagaggccgt agccactgcc
     3121 gacgtcgcag attgtcacct gattgcccgt cgtcgatgga gaggcgagca cgtagcgatc
     3181 gtctagggcg gatctgatga tcccgtcgag gcgttcccga tcccagacgg cgccgacttc
     3241 gcgccatcgc tcgatgaggc tgcgcaacgg atgagaaggt cctaggtcgg ccggtgtgat
     3301 gcggcggcgc agccggtgtc gcgccagccg gtcttgtgcg gtcctgatct gctggaacga
     3361 ggccgcaatc aggtcgagat gnnnnnnnnn nnnnncgcgc gggtgccacg tcccgttgta
     3421 cgtcgtcatg gcgaagcggc ggaaaggccg atcgctgatc cagctgccgg cggcgaccag
     3481 cgcgagctcg ctcgcccggg tggggcgaaa gcggagctcg acgccgtcgt caccgatgcc
     3541 gcggacctcg gcaaagccga tgttcctcaa gatgaagtcg gtgaaatccc cgccggcatg
     3601 aattccgccc cagcgctgcc ggaggatggg cgactcgctg tcccagggct gacctgatgt
     3661 atcgaacaac agggttccca actgcagctc ccgcatgaat caaggctcac gatggactat
     3721 acccgctgtg gtagaaatgt acagtgcccc caccagatca gtccgggcgt cgttcggcca
     3781 gcaagaatcg cgcctcggag cgcagcgcag ctacatcccg cgcggcctca gtccggtcga
     3841 ggcgcctcgc tggcagcggg atgtcgagcc gacgggtttc caccagcggc aatctcagcg
     3901 tgtatagaac gggcactgtg ccgtggaggc gcgcggcggc ggcgccgggc tcacgccgcg
     3961 tgctcgtcag gacggctcgg gcattctcat taacgaggcg ctcagcctgt gttgatacga
     4021 tatccgcgag gattgtgagg ctgccaatgt tccgcgcgca caggcaatgg cacgtatcgc
     4081 cgacgacggt cacggccgcg ggtcggtcaa taccgcgcgc cgacattgtc gcgattgacg
     4141 tcgagttgct cgaggcgcgg gatacaggag gggcgaagct gctggccatt gcctatatgc
     4201 tggcgacggc cgccattctg atcggcgtgt tcgagttcgg gttcagccaa cgctatctgc
     4261 ttgcggcggg tctatgcggc atgatcgggt tgagtggtct gcaagacgca tggcaggcga
     4321 cgaccgccag gacctatgag gtctccatcc gtcttgcttc cggcgagacc gcacgtgtga
     4381 cgcttgccga tgagctcgag gcgcgcggtc tcatggcggc aatctcggcg ccggcctgag
     4441 tgccgagcgc ttggcgcggc cccattctcc tgcgcaccgc gcttgtggat cgagacttgt
     4501 ggggcaggcg cgtttgagct aatcacgcgg cgtgccgggt taacgaccgc agatcgttga
     4561 aggccagctg ccgtagctca gtggtagagc actcccttgg taagggagag gtcgtgagtt
     4621 caatcctcac tggcagcacc atttcagtca ttcttcttcg tgccgattgg cacgtcaacc
     4681 gccgcgcagg cggtagatcg ccgtgtggag cacgcgagcg acggggcggg tgtcggcggt
     4741 cacgaggaca tcgagatcga cgaggtggtg gcccttcttc ttgtagttgc gctcgatcct
     4801 cgcgcggccc tcgagcgtct cgccgacgcg ggcgagcgag aggttctgca cctggctgcc
     4861 cgtgtgcacc cacggcggca gcagaacgtt ctccttgaga accagattgc agacgcgcag
     4921 gatctggccg gggtgaacga tgccgtcacg ctcgtagagg tcgtgctgct cgcgcacggc
     4981 agccaggtag tcggcgagtt cggccgcgcc gatcgcttgg ggcggtgatc gcagcagccg
     5041 gccctcggcg aggctcgtct cgtcggccgg gggacggtcc accagcggcg aggcgatcgc
     5101 ggccccgtcg agatggccgc gagcatctgt cgcgggtgcc ctgatgtcgg cgcgggccgt
     5161 cgccgagagc gtgccgttgc aggcgacgtg gagcgcgacg gcctcctcgc cagggacgtc
     5221 gagcgcggtg acgatcgctg tcaggccatc atagacgggc ttcaggaacc ggatcgacat
     5281 cgagccgccc tcgagccagg accggcccca gcgcgcaacg gcgaggtgcg tcgcataggc
     5341 atagacctcg acgcccggca cgagcccgcc ttcgaacccg aagcgccgcg ccacgctgtc
     5401 gtcgtggatc ttgttctcgg aggcctgcga gaggttgtag gcctctacgg cgtaggtcgc
     5461 ttggatcatc ggcggctcca gttccggcac gtgttgagca gggtagaccg gaacgtagac
     5521 ccggaccaac acggggaagc ggcatgccgc gcccaaaatt caggcgacgt gcagctcggt
     5581 catcatgccg gtcgccatgt gatagaggtg gtggcagtgg aggaaccact ggcccttgtg
     5641 ctcggcatcg agggcgattg tcaccggcag gttcggggga acgattaccg tgtcacgcat
     5701 tgcgccggcg aagcgccgcc cgccgatcgc gaccacctgg aagtgatggc cgtgcaagtg
     5761 catgggatgc atcatgccgg ttgggttcgc gaaagagatc tcgacgcgct cgcctcggcc
     5821 gaccttcaat ggctcgtgct cgccgtggat gcggttgttg atggtccagc ggtagttgtc
     5881 gccctcaccg agcgtaaggt tgaatgcctt gtcggcggca cgcgcggtga gtggggccga
     5941 ggcgcgcacg agggcctcga acgccgtgtc gatcaagggc cgcgctgcga gagcgctcgt
     6001 cgatcgccgt gttatgctgg cgcccacggt cgcaaggatg atgcccgtct ggaagcggac
     6061 gccctcgaca cctgcgatga cagggaaggc gcctcccgag ttcgggatcg tgacgacgag
     6121 atcgacgcgc tggccctggg cgagcggata gctcgctgct gagatcggcg cgcaggcatt
     6181 gccatcgacc gcaatgcagg ccgatttcat gccgggtgtc gtgatgaaga aggccgtcgc
     6241 cgtagcgccg ttgatgatgc gcagtcggac gcgcgccccc ttggcgacgc gcacgacctc
     6301 cgggtcgtcg aaggtgcgct cgttggcgag ataggcgtcg taagtgacgt cgttggcgtg
     6361 gaccatgccg ggccggtggc catgtgctgc cgagcccttc ggcgcgcctg tggccgcgga
     6421 ccggctgccg gtgcgcgacc ccgcccctga tccgctgcgg gagccctcgg atgcgacggg
     6481 cgcgcctttc gacggagcgg cgtcatggct ggagtgcacg cctgcaccac cgagacccgc
     6541 gagaatttcc tcaggcgaac ggaacgagaa gtcgtgcagc atcacgacga cgtcctggac
     6601 gtcgcccgcg ttcttctcgc gcgcaatcat tggggccgcg agcagcagct gctcggagag
     6661 ggtatgcgag tgcatccagt gggtgccggg cgtcagctcg aaatcaaagg tgtcgctgcc
     6721 gcctatgggg aggctgccac cgcccggccg cgcccgatcc tggtcaaacg gcgccttcac
     6781 ctggccatgc caatgcagct gcagcggctc gtgcgaggca ttcacgactg ttccattgaa
     6841 ccggtcaccc tcggtggcga ggatgccgtt ccgaccgcct gggccggcca catggaagac
     6901 ctttgcggcc ttcttgttca cctcgatggt gcgggtctct attctgagga ccggtggagc
     6961 cccttgggct tcggcccgtc cgggcccgaa cagcagggcg ccgctcgcca gggcggacga
     7021 tccgagaaac ttgcgacgat caagaactgg ctggcgcggt ttcatggcgt cctcgcgctg
     7081 ggttgatgaa ttgcccttcg cagatgagtt ggcggagtgt ggcttcaagg ccttgtgtga
     7141 tcacaatctc gtttcggatg tgcgccgcgg tgccgcgcgt caacggagca cgatccgccc
     7201 ctcgatccgg gcgccgatcg tgccgaggga gcggatatgg ctcatcacca cgctcgccat
     7261 gagcttgccg tccgtcttgc ctatgagggt gcggccgcgg gcgagcgcgc tgtagccgtc
     7321 accaccggcg agcatgaaat cattggatgc gaccttgtac ttccctgctg ggtcgatcgg
     7381 cttgccgtcg cgctcgattg cgacgacgcg ctcgccgctc ttggccttct cgttgacgac
     7441 gatcgtgagg cccgatacgt gtgggaaacg accgccacga ttgtcgaggt ctgagacgcc
     7501 attttcgaga gcgtccttca gatcacgacc ggtgatctcg accatcacgg tgcggtttcc
     7561 gaagggcatc tcggagagca cgtcgcggcg cgtgatgacg tgtcccgcag ggtagaggcg
     7621 attgcccctg agcgcgccgc cgttggtgag gccgatatcg gcgccggtcg agaggcggat
     7681 cgcgtcggcg acgatattgg cgaacacggc ttcttgcgat cgcactgtcg ccgagcgcgt
     7741 gtcgagctcg acgccagcct tgccgatcgc cacgtcgagc tcgcggccga gttcggcctc
     7801 gagccggtcg acgatcttct gcgcggccgg gtccggcgcg acgagcgcgc tgtcgatcac
     7861 gcggaagcgg ggccgccagg tgaccttgcg ccgcccgtcc tgttgcgaga cagccgcgaa
     7921 gatgtcgatc gcggtcacgt actcgccctc ctcgccgctc tccaccatga ccacgcgatt
     7981 gtcataggcg aggcggagat cgtgatcgtg tccggtcaac aggacatcga cgagcttcga
     8041 gcgcatgatc tcgaggtcgc gctcgaagtc cgtatgggtg acggcgacga cgaggtcggc
     8101 tcccgctgcg cggagcagct tcgcctgggt acgcaccgtc tccatctcgt cgaggaactt
     8161 gagggggccg ctttgcgaca tgaacggcgt cgtcgcaagc gccacgccga agacgccgac
     8221 cttgatcggg cccgcctcga ggagacggtt gtcgtcgtgg ccctggagcg gcttgccatc
     8281 ggcgccgcgc agattggcgg cgagataggg gaagcgcgat tctccgcgcc ggcgcatgta
     8341 gacgtccggg ccgaaatcga attcgtggtt gccagggacg aagacgtccg gcggcaccac
     8401 gttcaaaagc tcgaccgtgt gtgcgccctg gtcgaagccc gacatgagcg atggagagaa
     8461 catgtcgccg gcatgggcgt agaggagggg cacgccctta gcccgctcgg cgcgtgcgat
     8521 cgcggcaagg cgcgcgaagc cgccgcgccc atcgacgccg ctcatcttgt aaatgtcgtt
     8581 gacaagaagg agggtgaggg tcgggcgcgg acctgccgct gccgcaccga atgactgggc
     8641 cgctgcgggt tctgccccga cgccgagggg caggccggcg ccgaggctgg cagcggcggc
     8701 gagtgtcagg gcttcgcggc gggagacgtc agtcatggat gggcctcggg attggcaact
     8761 gcgctcggac cctactgcaa ccgttcctga aacgttgcag aaaactgcat ttggccggtc
     8821 agccgagagc gggccaggaa ggcggcgaca gtgagtctca acgagcactg gatcgacaag
     8881 atacccggcc tgtcggggct tgccccggat ctccgtcaag cgatcgcggc agcgggccgc
     8941 gtgctgcgtc ttcccaaggg aagccgcatc ttcggacccg gtcaggcgcc gcagaactat
     9001 ctcctgctcc tcgaggggga catccgcgtg agccaggtcg gcgagagtgg tcgcgagatc
     9061 gtgctctatc gcgtacttgc gggcgaaagc tgcgcgctga cgacgggctg cctgctcggc
     9121 ggcggcgatt atacggccga ggcgacggcc gacaccgacg tcgaggccgt ggcgatcccg
     9181 cgcacgcttt ttgatgatct gatcgcgcgc tcggccgagt tccgccgctt cgtcatctca
     9241 gccttcagcc agcgggtgac ggacctcttc aagctcgtcg aggagatcgc cttccagcgc
     9301 atcgacgtgc gcctcgccca caagctgatc gagcttgccg gagcaggtga tgaggtcgcg
     9361 atgacgcatc agcagatcgc ggccgagctc ggcacggccc gcgaggtcgt gagccgccac
     9421 gtgcacgagc tgcagcggcg cggcacggtc gtctcgaacc ggggcaacat ccgcatcgcc
     9481 gaccggagcg cgctcgagcg gcttgccggc ggcagttgat ctcgatcaat cgagccttcg
     9541 ccccgcgtga cttagttaca gatgcgacgg tgcgcgcccg atagcctcat cctgtcacca
     9601 actggagcag gagacggcgt catgaccaat gtcggcagca tcgatcgcat cctccgcgtc
     9661 atcgtcggcg cgggcctgat cagtcttgtc ttcttcggcc cgcaaaccaa ctggggctgg
     9721 atcggcgtcg tgccgctcct gacgtcgctc atcggctact gccccgcgta cacgttgctc
     9781 ggtgtaagca cctgtccgct ggatcgcagc aagcgcggct gaggcgttcg accggaagcg
     9841 gcgtcgccac tgaggccgct ctcgcccttg ccaccaacaa tcgagaccac gcccatgacc
     9901 ctactcactc agaaggcctg gtcgccgtac cttgcaggcg cggtgatcgg cctcctgcag
     9961 atcccgacct tcatcctgat ggataccgca ctcggcgcct cgtcctcgtt cgtcaccgcc
    10021 gcagcgcaca tcctctcgtt cgtagacgcc ggcagcacgc aggtgaaata tttcgcgagc
    10081 cacatgtggg gcgcgaagaa ctggtggcag gttgccgtcg tcatcggcat cgcgctcggc
    10141 gccttcatct cgatgcggct ttccggcgcg aaccggcaga cgatctcgcc ggtctggaac
    10201 cgcacaaccg gcacgacgtc gctcgcagcg agggcgccga tcgccttcct cggcggcttc
    10261 atcatgctgt tcggcgcgcg gattgcgggc ggctgcacct cgggccacgg cgtctcgggc
    10321 atggcgcagc ttgcggtcgg ctccacgctc gcggtcgctg ccatgttcgc aggcggcatc
    10381 atcaccgcca tgctgctgag acgcagctga tcacggagag actctcatga ccgtactctc
    10441 tggaattctg atcgggctcg cgaccggcgt cgtgtttggc ttcgcgctcg agaagtcgcg
    10501 cgtcttcgag cccggcgtca ttgtcgggca aatgcagctg cgctcgacgc tcatgctgaa
    10561 ggtgttcctt gccgcggtgg cgacgggtct cttggtgctc gccgtcatga atggcgtcta
    10621 tggcgtgaag ctttcgctga agccgctgct ctggcgtgcg gatatcatcg gcgggctcat
    10681 cctcggtgtc ggcatcacca tctcgggtgc ctgtccgggc acgtcgctcg cacaactcgg
    10741 cgcgggctat cgggacgcct tcttcgtcgt cctcggcggc atcgccggtg ccatcgccta
    10801 cggctacaac gacgcggcca tcacgaagct ctttgccgag agcggacaga agctcggctt
    10861 cgacaagctc cttggaatgc ccttctggct gacggccgtg atactggccg ctgtgctcat
    10921 cgtggtgctc gttgctctcg agatcatgct gccgtggcga agcgaggtcg gcgacgatca
    10981 tgatggcgtc accgaccgcc acgcgggacg gctcgtcgct gcggagtaag tctcgctccc
    11041 cgctcaagcc aaggtcgcac cctggatcgc gacccagact cggtccgggg tcagcggcat
    11101 ctcgatgtgg ttgacaccga gcggggccag cgcatcgcag accgcgttta cggcggccac
    11161 catcgagccg accgtgcccg cctcgccgac gcccttggcc ccaagcgggt tgaccgccgt
    11221 ggggacctcg ttcgtgccga ccgtgatcgc cgagagatcg tcggcgcggg gcatggtgta
    11281 gtccatgaac gacgccgtca tcagctggcc gctctcggga tcgtagctca tgcgctcgaa
    11341 tagagcctgg ccgacgccct gggcgacgcc gccgtggatc tgccccttca tcagcgtcgg
    11401 gttcatgacg cgcccgacat cctcgaccac cgtgtagcgc ttgatctcgg tctcgcccgt
    11461 cacggggtcg acctcgacct cgcagaggtg gcagccgttc gggaaggtca ccttggtcgg
    11521 ctggaactcg gcaccggcgg cgagcggctc gcccttgtcg gcggcgcggc gggcgacgtc
    11581 gccgagcgcg acggaacggt ccgtgccgac gacggtgaag cgtccgtcgg caaagcggag
    11641 gtcgccggcc gctgtctcga gcatgtctga ggcgacgacc tttgccttct cgatgacggc
    11701 atcgagtgcc tggagcgtcg ccgtgccgcc gacgcagagc gcgctcgagc cgccgctgcc
    11761 gcgaccgtgg ccaatggcgt cggtgtcgcc ctggcgatag gtgatctgtg aggccggcag
    11821 gccaagcttg ctcgccgcaa gctcgacgaa ggtcgtctcg atgccctggc cggtcgaaag
    11881 tgcgccggcc gtgacggtaa ccgtgccgtc gccggcgatt gaaagcatcg ctgcatcctt
    11941 gccgggtttc aggaacgggc cgcctgcaac ctcgaccggg ttggcgatgc cgaggccgag
    12001 cagcttgccg cggctcttgg catcggcccg tcgggcctcg aagccggatc ggtccgcgag
    12061 cctggcagcc tccgtcatgt tccgcgggaa gtttccgcaa tcgtaagtga agatgaagcc
    12121 cgtcgcgtag ggcatcgatt ccggctgcac gaggttgcgc cagcgcagct cgaaggggtc
    12181 gatccccatc tgccgcgcgg cgatgtcgat gacgcgctcg accgcgtagg tcgcctcggg
    12241 gcgtccggcg ccgcgatagg gggcggtcac ctgcgtgttg gtgaggacgc ccgtgatctc
    12301 ggcggcaacg atcggcgtgc ggtagacacc cgagatgcca ccgatattgt tgaggagccc
    12361 catcgagcgg ccggttagaa agcagccgag attgatctcg cagttgatct tgagcgccag
    12421 aaactgcccc tcgccgtcga gggcgagggt cgcctcgata ccgacgtcgc gaccgtgctc
    12481 gtccgagagg aagccttcga gccggtcaga aatccagcgc acgggccggc cgaggcggcg
    12541 ggcggcggcg agcaccagga cgtcctcgtt atagacaccg ctcttcatgc cgaacgagcc
    12601 gccgacatcg ccgacgacga tgcggagctt gtcgggcgtg accttgagga gcggcgcgag
    12661 gctccctttg aggccatgcg ggccctggtt cgagacgtga agaacaaggc gacccgcgct
    12721 gtcgacatag ccgagggcgc cgcgcggctc gatcgagttg gcgttgacgc gcgtgacgtg
    12781 gctcttcagt cgcgcgacat gcttggcgcc ggcaatgacc ttgtcggcgg cgccgagatc
    12841 gccacgcgtc cagtagagcg cgacgttgtc aggagcggcc ggccagatcg ccggagcccc
    12901 gggtgcgcgg gcctgggccg tcgtcacgac ggccgggaga tcgccgtagt ctggcacgac
    12961 gagctcagcg gcgtcctgtg cggcgcggag cgaggttgcg accacgaaag cgatcggctc
    13021 accgacgaag cgcacgcggt cgcgtgcgag taccgggcgg cgggcttcgg gcgccttcga
    13081 gccgtcgggc cgcttctgct ccacgacagg actgatttcg gtgatgccat cggcggcgag
    13141 gtcggcggcg gtgagcacag cgaggacgcc gggcgcggcc ttggcggcgg cagtatcgat
    13201 gcctctgagc tcggcgtgcg gcaggctcga gcggacgacg acgccgtagg ccatgtccgg
    13261 catcattatg tcggccacat agcggccgcg gcccgtcacc atgcgctcgt cctcgcggcg
    13321 gacccgcttg gcctcattcg tcgtcatcgc atcatccccg aaagtggctt tggcgcacat
    13381 tacagcagga gttgtcgctg tcgatgggtg cgacgacatg caagcctggc tcgaccagtg
    13441 gttgaagcgt tggcggcgct cgtctaccgt ccggcggcaa ttcgggtctc atgaggatac
    13501 gtcgcgcgtc ggcgcggcgg aggaggaacg gtgatcgatt tctacacttg gtccacgccg
    13561 aacggccgca aggtctccat catgctcgag gagatcgggc tcccctatac gacgcacgcc
    13621 gtcgacatca acaaggacga gcagttcaag cccgagttcc tgaagatcag cccgaacaac
    13681 cgcattccgg caatcgtcga ccgcgacaat ggcctcatgc tcttcgagtc gggcgcgatc
    13741 ctcatgtatc tcgcggagaa gacagggaag ctcgtgccca aggacgagaa gggccgctgg
    13801 catgccgtgc agtggctcat gtggcagatg ggcggcgtcg gcccgatgct cggccaggtg
    13861 catcactttg tgaagtacaa tgccggcaag gcgccctacg ccgaggagcg cttcctcaag
    13921 gaaggaaagc gcctctacgg cgtgctcgat cgccggctcg ccgaggcgcc atacgtcgcc
    13981 ggcgactact cctatgccga catcgcgatc tggccatgga tctcgcgctt cgagtggcag
    14041 acgatcgaca tgaaggcctt cccgaacgta atgcgctggt acaaggcgat cgcggcgcgg
    14101 cccgccgtgc agcgcggcta tgatgtgccg gtgaagcagc ctggcattcc gatgccggcg
    14161 gcctgagcgg gccgcttggg cggcgcattg gctgtcggtc gtgtcccgcc gcaggtgggt
    14221 tggcaccggt gatctcgcgc gattgtgcgc cacaaccgcg gccagaaatg gtaaatgtcg
    14281 ctgcaaccga ccgggcgagg catcgttcgg tcccgtaatt cttgtcgccg gagtcccggc
    14341 acgaaggctc tcagatcact gacgcgagga ctgacatgaa gacgagcagg attttctcgg
    14401 gcgtgatagc gcctgcatcg acgcctttcg gcgaggacgg catgccggac gaggaccgct
    14461 tcatcgagca ctgccactgg ctgctcgagg acggctgcac ggcgctcgcc ccgttcggga
    14521 cgacgagcga ggccaactcc ctgggactcg atgagcgcat ggagctgctc gaggccctcg
    14581 tcgagagcga catcgatccg gcaaagctga tgcccggcac cggcacgtgc tcggtcaccg
    14641 ataccatcaa cctcaccgat cacgcgctcg atctcggctg cgggggcgtg ctcatgctgc
    14701 cgccgttcta ctacaagaac ccgagcgagg acgggctgtt ccggtttttt gcggatgtga
    14761 tcgaggagat cagcgacgac gacctgaagg tctatttgta ccacatcccg ccgatcgctc
    14821 aggtcggctt ctcgctgccg ctgatcgagc gcctgatcaa ggcctatccg aagacgatcg
    14881 ccggcctgaa ggacagctcc ggcgactggt cgaacacaaa ggcgatcatc gaggcgttcc
    14941 cgatgctcga ggtgtttccg ggctcggagg ccttcctgct cgacgggctg aagattggcg
    15001 gggcgggctg catctcggcg agcgccaatg tcgcggccaa gaaactgcgt cagctgttcg
    15061 atggctggca gggtccggac gctgcacgtc tccacgagga gatcctcggg ctgcgcaaga
    15121 ccattcagtc ctacccgatg atcccgctgc tcaaggcgat catcggccat taccggcagg
    15181 accccggctg gagcgcggtt cgcccgccgt tcacgccgat gtcggcggcc gatacggcgg
    15241 cagcggtcaa gaagctcgcg gacacgcacg gcttcaagct cgatttcgcc gacgcggcct
    15301 gatcattgca acaccccaga aacagcgaac ccctccggtg ggtgcgaccg gaggggctcg
    15361 atgtgggtgc tgcagtcgtc tgaggcggtc cgggaggcag tcgggtgagg cgggcagtcg
    15421 gtcggtgcgt cggcagtcgg taaggttggc ggctactggc ccggggcgga aagcggcaag
    15481 gcttcggtgc tcgtcgtcag cgagaggccc atgaaggccg aggcggcggc gaagcagagc
    15541 agcgcgagca gcttagcagc gttcatcggt ctcggcccct tgactgatgc tgtcggcatc
    15601 gtgctacccg tgtggcagta gatgcttcgg cgtcatttga tgaccaagtc gtaaccgata
    15661 acagtttgtt agtcgatcgc ttgattataa caggtttaat cctaggatcg cggaattaat
    15721 acttcataaa tactcgttaa cgatccggtc gagaatcgcg tgcgcgcggt ttgcgcgtgc
    15781 gtggccagag gagtaggacg aaatgcggcc gaccatcgca tttgatcttg atggcttgtg
    15841 gcaacgtaca aagtgacgga aatccgattg gcaagcgtcg ggcggggcac tggcgccggc
    15901 cgagtatcgg gggcgcccat cgaaatggca gatcgcagca taacactcga gggcgggccg
    15961 ctcgggtttc tcctgatcca tggcctcggc ggcacacccg cagagctgcg gctcgtcgcg
    16021 caggccttga accgggccgg tcacaccgta cactgctgcc agcttgccgg gcactgcgcg
    16081 accgtgaacg atctgaggct gtcggtctgg caggaatggt atgcgagcgt cgaggcagcg
    16141 cacgatcgtt tgagcgccag ctgcaagacc atcgttgccg gcggcctctc catgggcggg
    16201 ctgctggcga tccacctcgc gcaccagcgc cccacgggtg tacaggggct cgcgatctat
    16261 gcgccgacgc tggtgctcga tggctggtcg gtgcccaagc tctggtcgta catcctgcgc
    16321 tgggtccggc cgatccccgg cttcaagtcg ggcttcatgg tgaaggagcg gccgccgtat
    16381 ggcctgaagg acgagcgcat tcgcgcaatc gtgctgcagc acatgcagag cggggacgcc
    16441 ggcgtcgcgg gcgtcttcga gacgccgatg cactcgatgg cgcagttcaa cgcgttttcg
    16501 gccgtcgtcc gccgcgagct tccgagcgtc aggcagcgtg ctctgatcct gcacccgcgc
    16561 gacgacgaca tggcgagcat cgagaactcg cactacctcc agcgcaatct cggcggcatc
    16621 gtcgaactgg tcgtgctgaa cgacagctat cacatgatca cgctcgaccg tcagcgccag
    16681 gtggtcatcg atcacacgct cgcgcttgcg agccgcctcg cgaagctcgg tccggagggc
    16741 ggcgcggccc gtctcgttcc cgctgcgcgc ggcgccgccg aatgagcggc gagggcgaac
    16801 tcgtctgccg ctgccacgcg agcgtcggcg agatcgaccg catggagtgg gatcgcctgt
    16861 ttcccggcga tccggaaggc cacacctatt acaccgcctg tgaggcggct ccgccgccgg
    16921 ccttcaccct gggtgccgtg acggtgaggc gaggggggcg tctcctagcg gctgccccga
    16981 catttcgact gatctatcgc ctcgatacgc cgctgcagga tcgcatgcgc gggctcggcg
    17041 attggctgca ccggcgattg ccgcgcctca tcaatctgcc gattctctgt ctcggctcgc
    17101 cgctggccga ccgttgccat ctggggctcg atccggcgct cactcccggc gaacgcgatg
    17161 cggtgatggc ggcactgctc acggggctcg atgccgaggc ggcgcgccat cacgcgaggg
    17221 tggtcgcggt gaaggacctc acggggcgcg atggcgagac gctgggtgcc cccttcaagc
    17281 gcgagggctt cacgcgaatc gcgagcctcc ccgtcgcggt actcgagctg ccgtttgcgg
    17341 acgagggcgc ctatctcgcg agcctctctt cggcaacacg caaggatatc cgccgcaagc
    17401 tcaagtcgag cggcgacctc cgcatcgaga ctcgctcctc gatcgccgga ctagaagccg
    17461 agattgcgag cctctacgag gagacacggc gccagagcgg cgtcgactac ggcgagttcg
    17521 agggattggc gcctagctac ttccccgccg tctccgtcca tctcggcgag cgcgcgcagt
    17581 ggatgctcta ctggctcggc gaccgcctga tagccttcaa tctgctgctt gtcggtgacg
    17641 agcgcgtcat cgacaagttc atcggcatgc gctaccccga ggcgcgtgag cacaatctct
    17701 atgccgtgag ctggatggcg aatgtgcgct tctgccttgc gcgcggcatc cggcgactgc
    17761 agaccgggca gacggcatac agctcgaaag tgcgctttgg cagcgcgctc gagcccatgt
    17821 tggtcttcgc acggcaccgc atcggcgtcg tgaacgcgct attcaaaatg ttcgggcccc
    17881 gcttcgcctt cgacaaatac gatccggagc tgaaggcgct ggcggcccgc aagggcgaag
    17941 cgtgacgccg gtcagccttt gagaaactcc gcggtgacgg cggccgtgtc tgcgccgagt
    18001 gccggggtcg cggacggcgg atcggtaacc ccgtcccaga gcgccggaag cgccggcacg
    18061 cgtgtcgggc cggacggcgt caccacatcc atcatgcgca agtgcgggtg ctcgagcagc
    18121 gccggcacgt cgttgacgcg gccgaacgcg atatcggccc tggacagcgc tgcgatgacg
    18181 ggctcgtggt cgcgcgcgcc gaaccagtcg gcgacgagcg tgtcggtcgc cgcgcggttg
    18241 gcgacgcgag ccgagctcga agagaagcgc ggatccgtga cggcgtccgg gcgctcgagc
    18301 acgcgctcgg cgagcgattt ccattcccgc tcgttctgga tcgagatcag gatcggcacg
    18361 gcatcgcgcg tcatgaacac gccgtagggc gcgagcgagg gatgcgcgag gccgatgcgg
    18421 gcgggcggct tgccggccgc gccgtacatg atcggcacgc ccatcagctc ggcgacgcag
    18481 tcgaacatgg agagctcaat ctcagcgccg tccccggtcc gtccgcgccg gagcagcgcc
    18541 tcgaggatcg cctcgtaggc gtgcaggccc gtcgcgatgt cgacgatcga gatgccgacg
    18601 cgcgagggcg cctcggggcc accggtcacg gcggcgaggc ccgcttccgc ctgcaccagg
    18661 aggtcgtagg ccttgcggtc agcataggga ccgcgctcgc cgtagcccga gatcgagcag
    18721 gtgatgaggc gtgggttgcg cctcctgagc tcgccgaccg ctaggccgag cttcgctagc
    18781 gcaccgggct tcaggttctg aatgaggacg tcggccttct ctgcgagtgc gaggatgagg
    18841 cgccggcctt
//
LOCUS       DAORNH010000048        18797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2254_length_18797_cov_2.659108, whole genome shotgun sequence.
ACCESSION   DAORNH010000048 DAORNH010000000
VERSION     DAORNH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2254_length_18797_cov_2.659108"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PK264_07060"
     CDS             <1..908
                     /locus_tag="PK264_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD75679.1"
                     /translation="NFRPGLLKRSGLDYDSVSKIAPHIVYCSLKGYLPGPYENRLALD
                     EVVQMMGGLAYMTGLPDRPMRAGASVNDVMGGMFGAIAIQGALAERARTGRGRYIQSA
                     LFENNVFLMAQAMMFETVTGMPSIPYSQKDSPWPVYDLFDTADGSKLFVTIVGEEQWV
                     AFCRAFGREGWLADPRLSTNQGRVDQRGWMIPEISGILRTWTSRDLAATLDRLGLPYA
                     PVNKPGDLSADPHLNASGGLMDIRLPDGRAARTPGLPLSIDGARLANRRNPPRIGEHT
                     REVLRELGYGANDIAAYEKQGVIAA"
     gene            997..2043
                     /locus_tag="PK264_07065"
     CDS             997..2043
                     /locus_tag="PK264_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75680.1"
                     /translation="MPVSRRTLMLGGGAAIGAGLLPTAVTAQAKTATLAFGPSTAVYG
                     LGMVAEAKGFFKEEGLDFKLVLGNAGTHGRQALAAGQALFAHGDASHPLQLSTRGKKS
                     KIILATQMIASISNIVVRKDLFDAGITTVEKLAAYKRPDGAKPIVAATAIGSGTWMYG
                     TYVFEANGLGDKVNWVAGGGPKTMFPGLETKQFDAIMAVPGWVIEAEKKGFGKVIYDT
                     SKAGVFQKEFGGTVPVLVVYALEETIQQDKAMVQAFVTGMLKGMKWVKATPIDDVYKL
                     VAEKHFKGVDPEAVKAELGFDKATWTYDGRIDEAAFARGGKVWYRKGSDIPESKFADL
                     VDMSFVEAAHAKLK"
     gene            2099..2902
                     /locus_tag="PK264_07070"
     CDS             2099..2902
                     /locus_tag="PK264_07070"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75681.1"
                     /translation="MEPSGSRVRISVTGLDKRYRSAGGEVNAVENVSFDVRQGEFVAL
                     LGPSGCGKSTILNMVAGLLERSGGTIGIDGENVRHGYVNRKVGYVFQRDTVFPWRTVE
                     ANIGYGLEIAGVARAERQARVERAIEVAGLSGFGRSFPRTLSGGMRQRVALMRTLIME
                     PEILLMDEPFGALDTHTKLEMHKTLLEIWERERQTVLFVTHDLGEALTLSSRIILLSA
                     RPGRLKEDFEVPFVRPRDAVSLRETAEFGRLYSHIWHSLGEEFRLTKAE"
     gene            2905..3756
                     /locus_tag="PK264_07075"
     CDS             2905..3756
                     /locus_tag="PK264_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD75682.1"
                     /translation="MKNRQLSILFWQIVILVVSLSIWQWGFDVAKALLPKPWVPKILD
                     PYFISKPTAIWTSFLKLGCFADKAGFAACYGENPNNLWLATLVTLKNMTWGFLWGTSL
                     GILTGLILGRSEYLSKIFEPYIVAMNSIPRIALVPLIILMFGLGDLSKVVTAGIVVFF
                     VVFFNTFEGTRSVDRDQIAAARLLGASELTVLRTVVIPSALAWVFASLTPAVSFALIG
                     VIVGEFIGAERGLGKLIIEAEARANAAEMMVAIFVMMIVGIVLAYIVRRIQSYLLRWQ
                     PQFESRA"
     gene            complement(3822..4010)
                     /locus_tag="PK264_07080"
     CDS             complement(3822..4010)
                     /locus_tag="PK264_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75683.1"
                     /translation="MNEVLLVIRHPAILAAAGEQFVEHAGGIFERDAVMPLWVRLCLL
                     VVPFEVVGDEACRSAAVG"
     gene            4170..4406
                     /locus_tag="PK264_07085"
     CDS             4170..4406
                     /locus_tag="PK264_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRD75684.1"
                     /translation="MSDAEWAASPCVPRVTVIRRALQLSQEAFAKRFQIPIGTLRDWE
                     QMRKEPDAAAKAYLKVIASDPEVVRQALAKRPAA"
     gene            complement(4595..5680)
                     /locus_tag="PK264_07090"
     CDS             complement(4595..5680)
                     /locus_tag="PK264_07090"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD75685.1"
                     /translation="MPNSTDATRPFGRRPTILLLAAPETSASILYGLFDVLTTVGSIF
                     PELVKGAPERALLDVRIVSADGRSFSCVGDIPVRPHCSITDFAAPASVPDAIVVCDMY
                     VATTAVPTGRYPRECAWLREMHAAGAFLTSVCSGSLVLADSGVLEGREATAHWAYGDM
                     FRRYFPRVTFRESAVLCLKEQDAGIVTAGGANAWNDLAFYLIARFCGYSHAIQTAKVF
                     LLSVHADSQCAYAVMTRPMDSKDAVVAECQAWIAENYEINSPVEQMARRSGLSARTFA
                     RRFQAATGYTPIDYVQALRIEEAKQELERDQRSVEDVAAMVGYEDDASFRRVFKQRVG
                     LTPAAYRKKFQSLSARAAAYELKRPKA"
     gene            5800..7194
                     /locus_tag="PK264_07095"
     CDS             5800..7194
                     /locus_tag="PK264_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD75686.1"
                     /translation="MDAPHFSLDQLGNGFAGTLLTPADAAFDDARRVWNVMIDRKPGL
                     IARCRGTADVIRAVRFAADNGLSVAIRGGGHNVAGHAVCDGNLMIDLSEMRAVHVDPV
                     RRIAIVEGGATWAEVDRETQAFGLATPGGLISETGVAGLTLSGGFGYLRGKHGLSIDN
                     LQAADVIVADGSLVRASTTENPELLWALKGGGGNFGVVVRFEFALHPVGPEVLFVAPI
                     YAVDNGPGPIRAWRDYIRQRDEDLAMICEFSTVPENDQFPQAYWGKKVFALIGIVTGE
                     PEHAESLAKPLRELGTLVTDFSGRMPYCDIQRLFDTQTPFGKMRCYWKSRLLTDLPDA
                     MIDLAMDNAVRAPSSNTISSLWNMGRAVNAVAANASAFGDRSMGWMYSVDGVWADASD
                     DDLNISWARTSWAKAERFGHADRIYLNFPGHDEGNVLTRQAFGDTYPRLVAVKRAYDP
                     RNMFRYNQNISPAE"
     gene            complement(7285..8055)
                     /gene="surE"
                     /locus_tag="PK264_07100"
     CDS             complement(7285..8055)
                     /gene="surE"
                     /locus_tag="PK264_07100"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRD75687.1"
                     /translation="MLILLSNDDGIDAPGLHALREIARDLAGPGGEVVVVAPETNQSG
                     AGHSLSLNEPLRMREVGEGAYAVRGTPTDSVIMALRHVLKGRKPDLLLSGINRGANMA
                     EDLTYSGTVAAAFEGTLLGVRSIALSLSFGAGPEPRWETPLAHGARVIRSLIAEPWAS
                     NVLMSINFPDRAPDAVEGVRATRQGRRDLALIDVEARRDTWGTPYYWLAYERRRSNPK
                     DDTDLWAVYNGYISVTPISLEMTHGPSLEGLVKRLVAG"
     gene            8235..>9720
                     /locus_tag="PK264_07105"
     CDS             8235..>9720
                     /locus_tag="PK264_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75688.1"
                     /translation="MALLGVGRGKVDARVNRPGLGRVIDYLFGRNALIAIASFMLLVI
                     SGYATWSGMQDFIVGVSTSSGAPAGREVPGGFTVSHDWLVVAITVALTFLMWLALRET
                     FGAQRRWHERLITLPLYLFLAMWSIGFGYGFWWSLISGVEATRSGLQSLQEDARDASA
                     AVAARLDAVKGQLDSVVSWSESQMAREETSGGSCGVPSGAGRGPLFNARRSVRDAVSA
                     IRDTVSKSWLAPVQSDLELLRASTSAIEGTTTEERQKRFEAAASTIRGKARSIATRSN
                     ELGRSTAADMLALANSVAIEPGKPGFSCFDPTLAQRLRQAAQTAAEPAKLQLREVAFN
                     EGPAGVANAVKNLWNNIGAYMKSLAIYVVSWGSKMGDWTSGGDPITGRDMIALLATIG
                     IDLGLLALAMLDPPQSAPVRRDGLADTLARLHIPPAAAVRQLSAVFQTAIGNAPGANL
                     DWVRRHFVFHNGASYFVIPNLYSAEKDNKDEELRALALNQLAGVF"
     assembly_gap    9721..9787
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9788..10198
                     /locus_tag="PK264_07110"
     CDS             <9788..10198
                     /locus_tag="PK264_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75689.1"
                     /translation="GEERRASYTDLTQLREEGREDLKTEPPTGILDKLRGRDPARVEN
                     PDRIRNHGLLSKAQRALDIAGWSARAQSDVEVFRLVDSEGLTPLLTLLNEASFMKGSA
                     LAEVAPEPPVAEGTGAATPLALPGRRRGDERHQT"
     gene            complement(10214..10435)
                     /locus_tag="PK264_07115"
     CDS             complement(10214..10435)
                     /locus_tag="PK264_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75690.1"
                     /translation="MSGTALLLLAMHVTASVVSPAGRTAFVTSPAMLAALVSVAFLAV
                     IAAYAATLHPRRRAVAQRRVVIRHRGPKG"
     gene            10659..11912
                     /locus_tag="PK264_07120"
     CDS             10659..11912
                     /locus_tag="PK264_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75691.1"
                     /translation="MSTYFFDQLETREPVAREQAMFGLLPSFARKVVDACPSWAEHLA
                     GHNLAGVTDREALAKLPLMRKSTIKALQGRRPPLGGLATPDASRWGRIFMSPGPIFEP
                     EGASTDSWRTARALFAAGIRPGDMVHNTFAYHLTPGGWIVDSGARALGCTVIPAGPGN
                     NEQQLEIIAHLRPNVYIGVPDYLKILLDKAKEADRDASSFKKAFVSGGALFPSLRAEY
                     KSRGISCMQCYATADLGLVAYESAGPDGSIEGMVVDEGVIVEIVRPGTGDPVPEGEVG
                     EIVVTSFNRDYPMIRLATGDLSAVLAGKSSCGRTNMRIKGWLGRADQTTKVKGMFVHP
                     ESIAEVAKRHPELGRLRLVVTRQGEQDVMTLKAEAKGAATPELMSAVAKTLQDITKMR
                     GEVAFVAPGSLPNDGKVIADDRTYG"
     gene            complement(11940..14060)
                     /locus_tag="PK264_07125"
     CDS             complement(11940..14060)
                     /locus_tag="PK264_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD75692.1"
                     /translation="MRPSRRPGGLKGLRALRLAIAAMLVLLVGLETALAKRLALVVGI
                     DRYDNLPAHQQLERAVGDARTIAKTLRDLQFDVIAAENTGREAFFAAWARFLASISPG
                     DTAAIFFAGHGVEMRGANFLVPRDAPAAEKGEIVLRNGAIALATLLDELDERKAATAL
                     VILDACRNNPFSVSGRSVGGTRGLARVDERPGTLILYSAGAGQTALDRVPGSPGAPNS
                     VFTHTLAPLLRTEGLTLLEVAIRTQRQVDALTRRADHMQTPAFYSQLLRDVYLAGPPK
                     AATALSDADAKRKAEEAEFARRVEAEVKRRDEERRKTAEASKAEAPKPVAPPARASGS
                     LKPDPFEPPVPGAADIAKALEIAEQVSSPLDKAGLLAAIAASYARAGRGAEARSVVGL
                     ALDLVRPEMQKSEGVSPQVSAVIGAMARVGLTGRAKELALNGIKDAGARESAIARMVV
                     VLAGVGETDEAAKLLALVKSDSNRIWSLTGLAIALHKNGESNESARLIAEATSLHRAM
                     TSSTAPYYTQTIAAALVRTERAREAIEVTRAIGSHAARRYASQSLGRDLVKLGMRAEA
                     VAAFELAIAAARTDSGSFLAEHIAEIAVAAARSGLEPEARRMLAEAETLARGLSDPFF
                     RASANFRIAGAKAALGHFEEAIALAKSVDRSILQGLAFGDIARALTSAGRTDQALQLV
                     MLVQDAKASEGVLRSVVYALLQSF"
     gene            complement(14149..14631)
                     /locus_tag="PK264_07130"
     CDS             complement(14149..14631)
                     /locus_tag="PK264_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75693.1"
                     /translation="MPAFELRRLRHTDRERFAALAGAYDVARCTSDIPFPFSAIEATS
                     WIDIRPGDLRLAIVVDGELVGAVGYFLRPSGIAELGYWIGMPWWGRGLATATSRVVVD
                     DAFRQTRIPAFSAHHFADNPASGRILMKLGFAVMGQGREFCRARSAAVDTVSYWLARA
                     "
     gene            complement(14674..14946)
                     /gene="rpmA"
                     /locus_tag="PK264_07135"
     CDS             complement(14674..14946)
                     /gene="rpmA"
                     /locus_tag="PK264_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRD75694.1"
                     /translation="MAHKKAGGSSRNGRDTAGRRLGVKKFGGESVVAGNILVRQRGTK
                     WHAGQNVGMGTDHTLFAKVDGSVEFATKRNGRVYISVKPPLPAAAE"
     gene            complement(14980..15294)
                     /gene="rplU"
                     /locus_tag="PK264_07140"
     CDS             complement(14980..15294)
                     /gene="rplU"
                     /locus_tag="PK264_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRD75695.1"
                     /translation="MYAVIKTGGKQYRVAADDVITIEKVVGEAGATVEFGEVLMLGGS
                     GEPKIGKPLVSGAKVTGEVVEQTRGPKLIAFKKRRRKNSRRKKGHRQDLTTVRIKSIT
                     GA"
     gene            15765..>17994
                     /gene="secA"
                     /locus_tag="PK264_07145"
     CDS             15765..>17994
                     /gene="secA"
                     /locus_tag="PK264_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRD75696.1"
                     /translation="MLSFSTFAAKIFGTSNDRKVKAYRPRVEAINALEPELQALSDAD
                     LRARTDMFKKQLADGATLESLLVPAFATVREAARRALGQRHFDVQLIGGMVLHDGKIS
                     EMKTGEGKTLVATAPVYLNALTGRGVHVVTVNDYLAKRDSEWMGAVYRFLGLSVGCIV
                     HELDDNQRRDQYDCDVTYGTNNEFGFDYLRDNMKMSAAEMVQFGRRPAELRGHYYAIV
                     DEVDSILVDEARTPLIISGPVEDRSELYTQIDALIPRIDKADYELDEKQKQVSFTEAG
                     NEKIEKLLDEAGLLKGPGLYDIENVTVVHHAQQALKAHKLFQRDRDYIVKDGQVVIID
                     EFTGRMMQGRRYSEGLHQALEAKEAVAIQPENQTLASITFQNYFRLFEKLAGMTGTAA
                     TEASEFMEIYGLDVVEIPTNRTVARLDMDDEVYRTAREKWNAIIRDVESARARKQPVL
                     VGTVSIEKSEQLSEQLKAKGVPHQVLNARYHEQEAFIIAQAGVPGAVTIATNMAGRGT
                     DIQLGGNVQMRIMQECEGLEGAAREKKIAEITEEVARFRELVLKAEDHVELEPAKGSK
                     PAKTVTYPGGLYVIGTERHESRRIDNQLRGRSGRQGDPGRTRFYLSLEDDLMRIFAAD
                     RLDGLLKRLGMQEGEAITSPLMNKALENAQKKVEARNFDSRKYVLKYDDVMNDQRKVI
                     FEQRVDIMGREDVSDTIIELRGQVIAELVSEHIPENAYAEQWNVEGLKAEVTRIFGLD
                     LPI"
     assembly_gap    17995..18037
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            18319..18797
                     /locus_tag="PK264_07150"
                     /pseudo
     CDS             18319..18797
                     /locus_tag="PK264_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950025.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3268 a         6299 c         5985 g         3135 t
ORIGIN      
        1 agaattttcg tcccggcctg ctgaagcgca gcgggctgga ctacgacagc gtgagcaaaa
       61 tcgcgccaca catcgtctac tgctcgctga aaggctatct gccgggtccc tacgagaacc
      121 gcctcgccct cgacgaggtc gtgcagatga tgggcggcct cgcctacatg accgggctac
      181 ctgatcgtcc gatgcgggcc ggtgcctccg tcaacgatgt catgggcggc atgttcggcg
      241 cgattgccat ccaaggcgcg ctcgccgagc gggcacgaac cggacggggg cgctacatcc
      301 agagcgcgct cttcgagaac aatgtcttcc tcatggcgca ggccatgatg ttcgagacag
      361 tcacgggtat gccgtcgatt ccctattccc agaaggacag cccctggccg gtctacgacc
      421 tcttcgatac cgccgacggc tcgaagctgt tcgtcacgat cgtcggcgag gaacagtggg
      481 tcgccttctg ccgcgccttc ggacgcgagg ggtggctcgc cgatccacgt ctgtcgacca
      541 accagggccg ggtcgaccag cgcggctgga tgatccccga gatctccggc atcctgcgca
      601 cctggacctc gcgtgatctc gccgcgacgc tcgatcggct ggggcttccc tatgcgccgg
      661 tgaacaagcc gggcgacctc tccgccgatc cgcatctcaa cgcctccggt gggctcatgg
      721 acatccggct ccccgacggg cgggcggcga gaacccccgg cctgccgctc tcgatcgacg
      781 gcgcgcggct agcgaacagg cgaaatccac cgcgcatcgg cgagcatacg cgtgaggtgc
      841 tgcgcgagct cggctacggc gcgaatgaca tagccgccta cgagaaacag ggtgtcatcg
      901 ccgcttgaat gccggcagcc gccgacgcat cacatcactg agaattcgcc aaccccgctc
      961 acatcaacga gcggcccatg atcagggagg aatgccatgc cagtctcacg tcgcactctg
     1021 atgctcggcg gcggcgccgc gatcggtgcc ggcctgctgc ccaccgcggt taccgcgcag
     1081 gcgaaaaccg cgacgctcgc cttcggcccg tcgaccgccg tctacgggct cggcatggtc
     1141 gcggaggcga agggcttctt caaggaggaa ggccttgatt tcaagctcgt cctcggcaat
     1201 gcgggcacgc acggccgcca ggcgctcgcg gctggccagg ccctcttcgc ccatggtgac
     1261 gcgagccacc cgctgcagct ctcgacgcgc ggcaagaaat cgaagatcat tcttgcgacg
     1321 cagatgatcg cctcgatttc gaacattgtc gtgaggaagg acctcttcga cgccggcatc
     1381 acgaccgtcg agaagcttgc ggcctacaag cgtccggacg gcgcgaagcc gatcgtcgcc
     1441 gcgactgcca tcggctccgg cacctggatg tacggcacct acgtcttcga ggcgaatggc
     1501 ctcggcgaca aggtcaactg ggtcgccggt ggcgggccga agaccatgtt ccccggcctc
     1561 gagacgaagc agttcgacgc catcatggcc gtgccgggct gggtcatcga ggcggagaag
     1621 aagggcttcg gcaaggtcat ctacgacacg tcgaaggcgg gtgtcttcca gaaggagttc
     1681 ggcgggacgg tccccgtgct cgtcgtctat gcactcgagg agacgatcca gcaggacaag
     1741 gccatggtgc aggccttcgt gaccggcatg ctcaagggta tgaagtgggt caaggccacg
     1801 ccaatcgacg acgtctacaa gctcgtcgcc gagaaacact tcaagggcgt cgaccccgag
     1861 gccgtcaagg ccgagctcgg cttcgacaaa gcgacatgga cttatgacgg gcgcatcgac
     1921 gaggcggcct tcgcgcgcgg cggcaaggtc tggtaccgca agggctccga catcccggaa
     1981 tcgaagttcg ccgaccttgt cgacatgagc ttcgtcgagg ccgcccacgc caagctgaag
     2041 tgaggcgccg gtgagcgtcg ccgaggccac tcgcgccacg acagcagcac ccgcgcgcgt
     2101 ggagccgtca ggttcgcgcg tgcgcatctc cgtcacgggg ctcgacaagc gctatcgatc
     2161 ggccggcggc gaggtgaacg cggtcgagaa cgtgtctttc gacgtcaggc agggcgagtt
     2221 tgtcgccctg ctcgggccgt cgggctgcgg caagtcaacg atcctcaaca tggtggcggg
     2281 cctgctcgag cggtcgggcg gcaccatcgg catcgacggc gagaatgtcc gccacggcta
     2341 cgtcaaccgc aaggtcggct acgtcttcca gcgcgacacg gtatttccct ggcggacggt
     2401 cgaagccaac atcggctacg gcctcgagat cgcgggcgtc gccagggccg agcggcaggc
     2461 gcgcgtcgag cgcgcgatcg aggtcgcggg cctctccggc ttcggccgca gctttccacg
     2521 cacgctgtcg ggcggcatgc gccagcgagt cgccctcatg cgcacgctca tcatggagcc
     2581 cgagatccta cttatggacg agccgttcgg cgcgctcgac acgcacacca agctcgagat
     2641 gcacaagacg ctcctcgaga tctgggagcg tgagcgccag accgtgctgt tcgtcacgca
     2701 tgacctcggc gaggcgctga cgctctcgag ccgcatcatc ctgctgtcgg cacggcccgg
     2761 ccgattgaaa gaggatttcg aggtgccgtt tgtgcgcccg cgcgatgccg tctcgctgcg
     2821 cgagacggcc gagttcggac ggctatactc ccacatctgg cactcccttg gtgaggagtt
     2881 ccggctgacg aaggcggaat gaccatgaag aaccgccagc tgtcgatcct gttctggcag
     2941 atcgtcatcc tcgtcgtctc gctctccatc tggcaatggg gtttcgacgt cgccaaggcg
     3001 ctgctgccga agccgtgggt cccgaagatt ctcgacccct atttcatctc caagccgacg
     3061 gcgatctgga cgagcttcct gaagctcggt tgctttgccg acaaggcagg cttcgccgcc
     3121 tgctacggcg agaacccgaa caacctctgg ctcgccacgc tggtcacgct gaagaacatg
     3181 acctggggct tcctctgggg cacgtcgctc ggcatcctga cggggctcat cctcggccgc
     3241 tccgagtatc tctcgaagat cttcgagccc tacatcgtcg ccatgaactc gatcccgcgc
     3301 atcgcgctcg tcccgctcat catcctgatg ttcgggctcg gcgacctctc gaaggtggtg
     3361 acggcgggaa ttgtcgtatt cttcgtcgtg ttcttcaata cgttcgaagg gacgcgctcc
     3421 gtcgaccgcg accagatcgc cgcggcccgc ctgctcggtg caagcgagct cacggtgctg
     3481 cgcaccgtcg tcatcccgtc ggcgctcgcc tgggtgttcg cgtcgctgac gccggccgtc
     3541 tcgttcgcgc tgatcggcgt catcgtcggc gagttcatcg gagccgagcg cggcctcggc
     3601 aagctcatca tcgaggcgga agcgcgcgcc aacgctgccg agatgatggt tgcgatcttc
     3661 gtcatgatga tcgtcggcat cgtgctcgcc tacatcgtgc gccgcatcca gtcgtatctg
     3721 ttgcgctggc agccgcagtt cgaaagccgc gcctgaggcg cgacatcacc gctgctcatc
     3781 ttcatgtgtc agtttgtcta tacggcttag cggtagcata ctcatccgac cgcagcggag
     3841 cggcacgctt catctccgac aacttcgaat gggacgacga gaaggcacag ccgaacccag
     3901 agcggcatga cggcgtcacg ttcgaagatg ccgcctgcgt gttcgacgaa ctgttcgcca
     3961 gcagccgcga ggatcgccgg gtggcgtatg acgagcagca cttcattcat tcagatcggc
     4021 atggcaggtg accgcatgct ggtcgtcgcc taatcgatgc gaggtgagcg catccgcatc
     4081 atctcggcgc gcagggcaga ggcagccgag cgacgccgat atcatgagga agattggtag
     4141 cacccttgag cgacaacgac agcaagccga tgtctgacgc agagtgggcc gcgtctccct
     4201 gtgttccgcg cgtcaccgtt attcgccgcg ccctgcagct ctcccaggag gcgttcgcca
     4261 agcgcttcca gatcccaatc ggcaccttgc gcgactggga gcagatgcgg aaggagcccg
     4321 acgcggcggc gaaggcctat ctgaaggtga tcgccagcga tccggaggtc gtccgccagg
     4381 cattggcgaa acgtccagcg gcgtgagccg ggtccgcccg ggccatcgcg agcgccgact
     4441 acgtgatgtg gatcgtcccg gaagcgcttg cctaggaagc cggcgccgag cgcctctaag
     4501 ccgccgtcgc cgccggcctc gccgccacca ccagccccgc ccgcgcggat ttctggcggc
     4561 ggtgaagggg gacggccggc gcgtgatgtc gctgtcatgc cttgggcctt ttgagctcgt
     4621 aggcagcagc ccgcgcagac aggctctgga actttttgcg gtaggccgct ggtgtcagtc
     4681 ctacgcgctg cttgaatacg cgcctgaagg aggcatcatc ctcgtatccg accatggccg
     4741 ccacgtcctc gacggagcgc tggtcgcgct ccagctcctg cttggcctct tctatgcgca
     4801 acgcctgcac gtaatcgatg ggcgtataac ccgtcgccgc ctggaaccgc cgcgcgaacg
     4861 tgcgcgcact tagtcccgag cgccgtgcca tctgctcgac cgggctattg atctcatagt
     4921 tttccgcgat ccacgcttgg cactcggcaa cgacagcgtc cttggaatcc atcggccggg
     4981 tcattacggc gtaggcgcac tgactatcgg cgtgcaccga aagaagaaag acctttgccg
     5041 tctggattgc gtggctgtaa ccgcagaagc gggcgatgag atagaatgcc agatcattcc
     5101 aggcgttggc cccgccggcc gtcacgatcc ctgcatcctg ctccttcaga cacagcaccg
     5161 cggactcgcg aaaagtgact ctcgggaagt accgacgaaa catatcgccg taggcccaat
     5221 gcgcggttgc ctctcggcct tccagtacac ccgaatccgc tagcacgagt gagccggagc
     5281 aaaccgacgt caggaaagcg ccagcagcat gcatctcgcg cagccaggcg cattcgcggg
     5341 gatagcggcc cgtcggaacg gccgtagtgg cgacgtacat gtcgcaaaca acgatggcgt
     5401 cgggcaccga cgccggcgcg gcaaaatcgg tgatagagca atgcggtcgc accgggatgt
     5461 ctccgacaca gctgaacgac ctgccgtcgg ccgagacgat cctgacatcg agcaatgcgc
     5521 gttccggcgc tcccttgacg agttcaggga aaatggagcc aaccgtcgtc agcacatcga
     5581 agagcccata gagaatcgag gccgacgtct ctggcgctgc gagcagcagt attgtggggc
     5641 gccgtccgaa gggtctggtg gcatctgtcg aattcggcac cgtttcgtcc ttttacacct
     5701 ggaccgttgt caattctggc actcatcagt cgagtaagcc actccatatc ttcgtccgac
     5761 acgcagagac tgggcgtcac cagagtatag gagatcgcca tggacgcccc tcatttttcg
     5821 ctcgatcagc ttggtaacgg cttcgcagga actcttctca cgccggccga tgcggcattc
     5881 gacgacgcgc gtcgcgtctg gaatgtcatg atcgaccgca agcctggcct gatcgcacgc
     5941 tgtcgtggca cggccgacgt cattcgcgct gtccgctttg ctgccgacaa cggcctgagt
     6001 gtcgccatcc gcggcggtgg ccacaacgtc gccggccacg cagtctgcga cggcaacctg
     6061 atgatcgatc tctccgagat gcgcgccgtg cacgtggacc cggtgcgccg cattgcgatc
     6121 gtcgaaggcg gcgcgacctg ggccgaggtc gatcgcgaga cccaggcttt tggcctcgct
     6181 actccaggcg ggctcatctc ggagaccggc gtcgcagggc tcacgctctc gggcgggttc
     6241 ggctacctgc gtggcaagca cggcctgtcg atcgacaacc ttcaggccgc cgacgtcatt
     6301 gtcgcggatg gcagcctcgt ccgtgccagc accaccgaga atcccgagct gctttgggcg
     6361 ctcaagggcg gtggcggaaa tttcggcgtg gtcgtccgct tcgagttcgc cctgcaccct
     6421 gtcggtcctg aggtgttgtt cgtcgcaccg atctatgcgg tcgacaacgg ccctggcccg
     6481 atcagagcct ggcgcgacta catccggcag cgtgacgagg atctcgccat gatctgcgag
     6541 ttctccacgg tccccgagaa tgaccagttc cctcaagcct actggggcaa aaaggtattc
     6601 gcgttgatcg gcatcgtaac cggagaacct gagcatgcgg agagcctcgc caaaccgctg
     6661 cgtgagcttg gaacgctggt gacagacttc tccggcagaa tgccgtattg cgacatccag
     6721 cggctgttcg acacgcaaac gccctttggc aaaatgcgtt gctactggaa atcacgcctt
     6781 ctaaccgatc taccagacgc aatgatcgac ctggcgatgg acaatgcagt gcgggctccg
     6841 tcctcgaaca cgatatcgtc gctttggaac atgggccgcg ccgtcaacgc cgttgcagcg
     6901 aatgcgtcgg cgttcggcga tcgctcgatg gggtggatgt actcggtcga cggcgtgtgg
     6961 gcggatgcgt cggacgacga cttgaacatc tcctgggcgc ggacgagctg ggcaaaggcc
     7021 gagcgcttcg gccatgccga ccgcatctat ctcaacttcc ctggacacga tgaaggcaac
     7081 gtcctcacca gacaggcatt cggcgacacc tatcctcggc ttgtcgcagt caagagggca
     7141 tacgatccaa ggaacatgtt ccggtacaat cagaacatat cgcctgcgga atgagctcta
     7201 cttggcccga tcgtccagac gcgatgctgg cggtgccgcg aggtcagccc tccggcacgg
     7261 ccggcgtcga cgtctggagc gctcttaccc cgccaccaac ctcttcacga gcccctcgag
     7321 gcttgggccg tgggtcatct cgagtgagat cggcgtcacc gagatgtagc cgttgtagac
     7381 ggcccacagg tccgtatcgt ccttcgggtt cgagcggcgg cgctcgtagg cgagccagta
     7441 gtagggcgtg ccccaggtgt cgcgccgcgc ctcgacgtcg atgagtgcca agtcgcgccg
     7501 cccctgccgg gtcgcccgca caccctcgac ggcatccggc gctcgatccg gaaagttgat
     7561 gctcatgagc acgttgctgg cccacggctc ggcgatcagc gaccggatca cgcgggcccc
     7621 gtgcgcgagc ggcgtctccc agcgcggctc gggcccggcg ccgaaggaga gcgagagcgc
     7681 gatcgagcgc acaccgagca gcgtcccctc gaaggccgcc gcgaccgtgc cggaataggt
     7741 caagtcctcg gccatgttgg caccgcgatt gatcccggag aggaggaggt cgggcttcct
     7801 ccccttgagc acgtgccgga gcgccatgat cacgctgtcc gtcggcgtgc cgcgcaccgc
     7861 gtaggcgccc tcgcccacct cccgcatgcg caggggctcg ttcagcgaca gcgagtgccc
     7921 ggcccccgac tggttcgtct cgggcgcgac cacgaccact tcgccgccgg gacccgccag
     7981 gtcacgggcg atctcgcgca gcgcgtgcag gcccggcgcg tcgatgccgt cgtcgttcga
     8041 gagcagaatg agcatcgccg tcctccacca tgcccgaaag ttagtggtga ggcagttgag
     8101 caaccgctgc aaggcgcgcg atcgcctcac ccggatcgca atcgccctcc ggtgtgtttt
     8161 gctcgcgaca ggccgcgctg gctccgggta catacaagcg gggagaaaca cccggaaggc
     8221 gaggggaggc gagcatggca ctgttgggcg ttggtcgtgg gaaggtcgat gcgcgggtca
     8281 accggccggg actcggccgc gtgatcgact acctttttgg ccgcaatgcg ctcatcgcca
     8341 tcgcctcgtt catgctgctc gtgatctcgg gctacgccac ctggagcggc atgcaggact
     8401 tcatcgtcgg cgtctcgacc tcgtcgggcg cgcccgcggg ccgcgaggtg ccaggcggct
     8461 ttacggtctc gcacgactgg ctggtcgtcg cgatcacggt cgccttgacc ttcctgatgt
     8521 ggcttgcgct ccgagagacg ttcggcgcgc agcgacgctg gcacgaacgg ctcatcacgc
     8581 tgccgctcta tctcttcctc gccatgtggt cgatcggctt cggctacgga ttctggtgga
     8641 gtctcatctc gggtgtcgag gcgacgcggt ctggtctgca gagcctgcag gaggatgcgc
     8701 gggacgcttc cgccgccgtc gccgcccgcc tcgacgccgt gaaaggccag ctcgacagcg
     8761 tcgtctcctg gtccgaaagc cagatggcgc gcgaagagac gagcgggggc agctgcggcg
     8821 tgccctccgg cgccggtcgc gggccattgt tcaatgcgcg ccgcagcgtg cgcgatgcgg
     8881 tctcggcgat ccgcgatacc gtgagcaaga gctggctcgc gcccgtccag tccgatctcg
     8941 aactcctgcg cgcgagcacc tcggcgatcg agggcacgac cacggaggag cgccagaagc
     9001 gtttcgaggc cgctgcctcg accatccgcg gcaaggcacg ctcgatcgcc acgcgctcga
     9061 acgagctcgg acgatcgacg gcggccgaca tgctggcgct cgccaacagc gttgcgatcg
     9121 agcctggaaa gccaggcttc agctgcttcg atccgaccct ggcgcagcgg ctgcggcagg
     9181 ccgcccagac ggccgccgaa cccgccaagc tgcagctccg cgaggtcgcc ttcaatgaag
     9241 gcccggccgg tgtagccaac gccgtcaaga acctctggaa caacattggc gcctatatga
     9301 agagcctcgc aatctacgtg gtctcctggg gctcgaagat gggcgattgg acctcgggcg
     9361 gcgatccgat cacgggccgc gacatgatcg cactgctcgc taccatcggc atcgatctcg
     9421 ggctcctcgc gctcgccatg ctcgatccgc cgcagtcggc accggtgcgc cgcgatggcc
     9481 tcgccgacac gctcgcccgc ctgcacatcc cgccggccgc cgccgttcgc cagctttcgg
     9541 ccgtcttcca gaccgccatc ggtaatgcgc caggcgccaa tctcgattgg gtgaggcggc
     9601 acttcgtctt ccacaacggt gcctcctact tcgtcatccc gaacctttac agcgccgaaa
     9661 aggacaacaa ggacgaggag ctgagagccc tcgcgctcaa ccagctggcg ggggtcttcg
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnggc gaggagcggc gggcgagcta caccgatctc acgcagctgc gcgaggaagg
     9841 ccgcgaggac ctcaagaccg agccgccgac cggcatcctc gacaagctcc gcggccgcga
     9901 tccggctcgc gtcgagaatc cggaccgcat tcgcaaccac ggccttctct cgaaggcgca
     9961 gcgtgccctc gacatcgccg gctggagcgc gcgtgcccag agcgatgtcg aggtgttccg
    10021 tctcgtcgac agcgaggggc tgacgccgct gctcacactc ctcaacgagg caagtttcat
    10081 gaagggctca gcccttgccg aggtggcacc ggagccgcct gttgcagagg ggacaggtgc
    10141 tgccacccca ctggcgctcc ccggtcggcg tcgcggtgac gagcgccatc agacctgatc
    10201 gacgccgatc gcgtcagccc ttgggaccgc gatggcggat caccacccgt cgttgggcga
    10261 ctgcccggcg gcgcggatgc agtgtcgcgg cgtaggcggc gatcacggcg agaaatgcca
    10321 cggacacgag ggcggcgagc attgccggcg acgtgacgaa cgctgtccgc ccggccgggc
    10381 tcaccacgct ggctgtcaca tgcatggcga ggaggagaag cgccgtacca ctcatatcgc
    10441 agccccaata ggatgatcgc gtcgcatttg cgtcgcttgc gaggcaaatg ctcaccggag
    10501 ttgtatcact tcgccgccgg aatgcacggc tcgcccgcga ccaaggttgc ccggcgatta
    10561 atcgctggcc caagccgggg cgctccgtgc cgcctcgata cattgtcgcc ttgcgaccct
    10621 cgtggggcca tgcgactgaa gccggggagg accgaccaat gagcacatat ttcttcgacc
    10681 agcttgaaac ccgcgagccg gtggcccgcg agcaggccat gttcggcctc ctgccgagct
    10741 tcgcccgaaa ggtagtcgac gcgtgcccga gctgggcgga gcacctcgcc ggccacaacc
    10801 tcgcgggggt cactgatcgc gaggctttgg cgaagctccc attgatgcgc aagtcgacca
    10861 tcaaggcgct gcagggccgc cgccccccgc tcggcgggct cgcgacgccg gacgcgagcc
    10921 gctggggccg catcttcatg tcgcccgggc cgatcttcga gcccgaaggc gcctccaccg
    10981 acagctggcg cacggcgcgc gccctctttg ctgccggcat ccggcccggc gacatggttc
    11041 acaacacctt cgcctatcac ctcacccccg gcggctggat cgtcgattcg ggcgcccgtg
    11101 cgctcggctg cacagtcatt ccggccggtc ccggcaacaa cgagcagcag ctcgagatca
    11161 tcgcccacct gaggccgaac gtgtacatcg gcgtcccgga ctatctgaag atcctcctcg
    11221 acaaggcgaa ggaggcggac cgcgacgctt cctcgttcaa gaaggccttc gtgtcgggcg
    11281 gcgcgctctt cccttcgctg cgcgccgaat acaagagccg cggtatctcc tgcatgcagt
    11341 gctacgcgac cgccgatctc ggcctcgtcg cctacgagag cgcaggcccg gatgggtcga
    11401 tcgagggcat ggtggtcgat gaaggcgtga tcgtcgagat cgtgcgcccg ggcacgggcg
    11461 atccggtacc cgagggcgag gtcggcgaga tcgtcgtcac ctcgttcaac cgcgactacc
    11521 cgatgatccg ccttgcgacc ggcgatctct ctgccgtgct cgctggcaag agctcgtgcg
    11581 gacgcaccaa catgcgcatc aagggctggc tcggccgtgc cgaccagacg accaaggtca
    11641 agggcatgtt cgtccatccg gagagcattg ccgaggtggc gaagcgccat cccgagctcg
    11701 gtcgcctccg tctcgtggtc acgcgccaag gtgagcagga cgtcatgacc ttgaaggccg
    11761 aggcgaaggg cgcagccacg cccgagctga tgtcagccgt cgcaaagaca ctccaggaca
    11821 tcaccaagat gcgcggcgag gtcgccttcg tcgcgcctgg ctcactcccg aacgacggca
    11881 aggtgatcgc ggatgatcgg acgtatgggt gagctaaggc ccgccaaacg cgcctagggt
    11941 cagaacgact gcagcaaggc gtagacgacc gacctgagga cgccctcact cgccttggcg
    12001 tcctgcacga gcatgacgag ctgcagcgcc tggtcggtgc ggccggcgct cgtcagcgcg
    12061 cgggcgatgt cgccgaacgc caagccctgc aggatgctcc gatcaaccga tttggcgagt
    12121 gcgatcgcct cctcgaaatg gccgagcgca gccttggcac ccgcgatcct gaagttggca
    12181 ctcgctcgga agaatggatc actgagccct ctcgcgagtg tctcggcctc ggcgagcatg
    12241 cgccgcgctt ctggttcgag gccggacctg gctgcagcaa cggcgatctc ggcgatatgc
    12301 tcggcgagga acgagcctga atctgttctt gcagcggcga ttgccaactc gaaggccgca
    12361 acagcctcgg cgcgcatccc aagcttcacc agatcgcgtc cgagcgactg cgacgcatag
    12421 cggcgcgccg cgtgcgatcc gatcgctcgc gtgacctcga tcgcttcccg agcccgctcc
    12481 gtccggacca gcgccgctgc gatcgtttgc gtgtagtatg gcgctgtgct cgaagtcatc
    12541 gcgcggtgga gcgacgtcgc ctcggcaatc aaccgtgcac tttcattcga ttcgccattc
    12601 ttgtgcaggg caattgcgag cccggtcagg ctccagatgc gattgctatc cgacttgacc
    12661 agggcgagca gctttgccgc ctcatctgtt tcccccacgc ctgcaagcac gaccaccatc
    12721 cgcgcgattg cgctttcccg ggctccggcg tccttgatcc cattgagagc cagctctttg
    12781 gccctgccgg tcaacccgac cctggccatt gcaccaatga ctgccgaaac ctgtggcgag
    12841 acgccctcgg atttctgcat ctccggacga acgaggtcga gcgcaagtcc aaccactgat
    12901 cttgcttcgg cgccgcggcc tgctctcgca taggatgcag cgatcgctgc aagcaggccg
    12961 gccttgtcga gcggcgagct gacctgctcc gcgatctcca aggctttcgc gatatcggcg
    13021 gcgcccggca ccggcggctc aaagggatca ggctttagtg agccgcttgc ccgtgcgggt
    13081 ggtgccaccg gctttggcgc ctctgccttg ctcgcctccg cggtcttgcg gcgctcctca
    13141 tcgcggcgct tcacctccgc ctcgacacgc cttgcgaatt ccgcttcctc ggccttgcgc
    13201 ttggcatcag cgtcggagag tgcggtcgca gccttcggcg gaccggcgag atagacatcc
    13261 ctcaggagct gcgaatagaa cgccggcgtc tgcatgtggt cggcccgccg cgtcagcgca
    13321 tcgacttgcc gctgtgtgcg gatcgcgacc tcgagcagcg tcaagccttc cgtgcgcagg
    13381 agcggcgcca gcgtgtgcgt gaagaccgag ttcggcgcgc cggggctccc cggcacgcgg
    13441 tccagcgccg tctggccggc gcctgccgag tagaggatca gcgtgccggg gcgctcgtcg
    13501 accctggcga ggccgcgcgt gccgccgacc gagcgaccgg aaacggagaa cgggttgttg
    13561 cggcaagcat cgagaatgac gagcgcggtg gcggccttgc gctcgtcgag ctcgtcgaga
    13621 agtgtcgcca gcgcgatcgc accgttgcgc agcacgatct cgcctttctc ggccgccggc
    13681 gcatcacgcg gcacgaggaa attcgcgccg cgcatctcga cgccgtgccc ggcaaagaag
    13741 atcgctgccg tgtctcccgg cgagatcgac gcgaggaagc gcgcccaggc ggcaaagaat
    13801 gcctctcgac cggtgttctc ggccgcaatg acgtcgaact ggagatcacg cagcgtcttg
    13861 gcgatcgtgc gagcatcacc gacggcgcgc tcgagctgct ggtgggcggg gaggttgtcg
    13921 taacggtcga tgccgacgac gagcgcgagc cgttttgcta aggcggtttc caagcccacc
    13981 aggaggacca gcatcgccgc aatggcgagg cgcagcgcgc gtagtccctt caaaccgccg
    14041 ggtcgtcgac ttggtcgcat ggctttgctc cgaaggccgc aatgcgcgaa cctacctcga
    14101 ctggagcgcg ccgagcaaat cgcaaatggc cgggcgacgt gccgaacctc atgccctcgc
    14161 gagccaatat gacaccgtat cgaccgccgc gctgcgtgcg cggcagaact cgcggccctg
    14221 gcccatgacg gcgaagccga gcttcatgag aatgcgtccc gacgcaggat tgtcggcgaa
    14281 gtggtgcgcg ctgaaggcag ggatgcgcgt ttggcggaag gcatcgtcca cgacgacgcg
    14341 gctcgttgcc gttgcgagcc cgcgtcccca ccacggcatg ccgatccagt agccgagctc
    14401 ggcgatgccg gacgggcgga ggaagtagcc gaccgcgccg acgagctcgc cgtcgacgac
    14461 gatcgctagc ctgagatcgc ccggccgaat gtcgatccag gacgttgcct cgatggcact
    14521 gaatggaaac ggaatgtcgc tggtgcagcg ggcgacgtca taggcaccgg cgagcgccgc
    14581 aaagcgctcg cggtccgtat gccgaagacg acgaagctcg aaggccggca ttgcgccaac
    14641 gctccgagct tcactcatgt gctggaccgg agcttattcc gccgcagccg gcagcggcgg
    14701 ctttaccgag atgtagacgc ggccgttgcg cttcgtcgcg aactcgacgc taccgtccac
    14761 ctttgcgaac agcgtgtgat cggtgcccat gccgacgttc tgccccgcgt gccacttggt
    14821 gccgcgctgg cggaccagga tgttgcccgc gacgaccgac tcgccgccga acttcttgac
    14881 gccgaggcgc cggcccgcgg tgtcgcggcc gttgcgggac gagccgcctg cctttttatg
    14941 tgccatggtc tactagcccg gattggagat gtgctgcgct caggcgccgg tgatcgactt
    15001 gatgcgaacg gtcgtcaggt cctgacgatg gcccttcttg cggcgcgagt tcttgcggcg
    15061 gcgcttcttg aaggcgatga gcttcggccc gcgcgtctgc tcgacgacct ctcccgtgac
    15121 tttggcgcca gaaacgaggg gcttgccgat cttcggctcg cccgagccgc caagcatcag
    15181 cacctcgccg aactcgactg tagcccccgc ctcgcccaca accttctcga tggtgatcac
    15241 atcgtcggca gccacacgat actgcttgcc gcctgtcttg atgacagcgt acatcttagc
    15301 ctctcgttct ccgcgcccta tggcgccgcc tcgaggcccc ggtgtcaccc ggttcgctcg
    15361 atcaggcgga cttcgacggc agccgcaatg gccgcaagcc tcggacagcg tgttcgctat
    15421 tgagagcgga cttatgtcgg cagcgccggc gaatgtcaaa cggattgcga ccgccgcgcc
    15481 tatgacgacc tatccatgac tgaccagtat ttgggcggtg cgcgttgaca acctccaccc
    15541 cctgtcctta tttcatggca acttggccgg ccggcgaagc ggatagcttg cctgtgcccg
    15601 atcacgctct cgatcgggct ctggggcgcg catcgtgtcg tgggggccat gcggctactc
    15661 gacgggtcgt cctcagcgcg ggccctcccg gatttgtgca acatgcggca cgagcctgcc
    15721 ccccgagcgc cggggcgcgg gccgattgat ttggggacca cctgatgctg tcgttcagca
    15781 cctttgcggc aaagatcttc ggcacctcga acgaccggaa ggtcaaggcc taccggccac
    15841 gtgtcgaggc catcaatgcc ctcgagcccg agttgcaggc gctcagcgac gccgatctgc
    15901 gcgcgcgcac cgacatgttc aagaagcagc tcgccgacgg ggcgaccctc gagagcctgc
    15961 tcgtcccggc cttcgccacg gtccgcgagg ccgcccgccg cgccctcggc cagcgccatt
    16021 tcgacgtcca gctgatcggt ggcatggtgc tgcacgacgg caagatctcc gagatgaaga
    16081 ccggcgaagg caagacgctg gtcgcgaccg cgcccgtcta cctcaacgcg ctgaccggcc
    16141 gcggcgtcca cgtcgtcacc gtcaacgact acctcgccaa gcgcgacagc gagtggatgg
    16201 gcgccgtcta ccgcttcctc ggcctctccg tcggctgcat cgtccacgag ctcgacgaca
    16261 accagcgccg cgaccagtac gactgcgatg tcacgtacgg caccaacaac gagttcggct
    16321 tcgactatct ccgcgacaac atgaagatga gcgccgcgga gatggtgcag ttcggccgcc
    16381 gcccggcgga gctgcgcggc cactactatg cgatcgtcga cgaggtcgat tcgatcctgg
    16441 tcgacgaggc gcgcacgccg ctcatcatct ccggccccgt cgaggaccgc tccgaactct
    16501 acactcagat cgacgcgctg atcccgcgca tcgacaaggc cgactacgag ctcgacgaga
    16561 agcagaagca ggtgtcgttt accgaggccg gcaacgagaa gattgagaag ctgctcgacg
    16621 aggcgggcct cctgaaggga ccaggtctct acgacatcga gaacgtcacc gtcgtccacc
    16681 acgcccagca ggccctgaag gcgcataagc tcttccagcg cgaccgcgac tacatcgtca
    16741 aagacggcca ggtcgtcatc atcgacgaat tcacgggccg catgatgcag ggccggcgct
    16801 actcggaagg cctccaccag gcgctcgagg ccaaggaagc cgtcgccatc cagcccgaga
    16861 accagacgct cgcctcgatc accttccaga attacttccg cctgttcgag aagctcgccg
    16921 gcatgaccgg taccgccgcc accgaggcaa gcgagttcat ggagatctat ggcctcgatg
    16981 tcgtcgagat cccgaccaac aggaccgtcg cccgcctcga catggacgac gaggtctatc
    17041 ggaccgcgcg cgagaagtgg aatgccatca tccgcgacgt cgagagcgcg cgcgcccgca
    17101 agcagcccgt gctcgtcggc accgtctcga tcgagaagag cgagcagctc tccgagcagc
    17161 tgaaggccaa aggcgtgccg catcaggtgc tgaacgcccg ctatcacgag caggaggcct
    17221 tcatcatcgc ccaggcgggc gtgccgggcg cggtgacgat cgccaccaat atggcgggcc
    17281 gcggcaccga catccagctc ggcggcaacg tgcagatgcg catcatgcag gagtgcgagg
    17341 gtctcgaagg ggccgcgcgc gagaagaaga tcgccgagat caccgaggag gtggcacgct
    17401 ttcgcgaact cgtcctcaag gccgaggatc atgtggagct cgagcccgcc aagggctcga
    17461 aacctgcaaa gactgtcacc tatccgggcg gcctctacgt catcggcacc gagcgccacg
    17521 agagccggcg catcgacaac cagttgcgcg gccgctccgg ccgccagggc gatcccggcc
    17581 gcacgcgctt ctacctgtcc ctcgaggacg acctgatgcg catcttcgcc gccgatcgcc
    17641 tcgacggcct cctgaagcgc ctcggcatgc aggaaggcga ggcgatcacc tcgccgctga
    17701 tgaacaaggc cctcgagaac gcgcagaaaa aggtcgaggc gcgcaacttc gacagccgca
    17761 agtacgtcct caagtacgac gacgtgatga acgaccagcg caaggtcatc ttcgagcagc
    17821 gcgtcgacat catgggccgc gaggacgttt ccgatacgat catcgagttg cgtggccagg
    17881 tgattgccga gctcgtcagc gagcacatcc cggaaaacgc ctacgccgag cagtggaacg
    17941 tcgagggcct caaggccgag gtgacgcgca tcttcgggct cgacctgccg atcgnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagc gcctcaccaa ggaggtcgag
    18061 gccaaggccg agaagatcgc cagcgacatc ggcgacaaga tcaagcgcgc ctacgacaac
    18121 gcgaagagcc tcgttggctt cgtcgagagc gagccggcgt tgaaggcgct gctcgagggc
    18181 gacccgacga gcaacctcac cgggatcggc cagttcctga tcaattcgga ccgcggcgag
    18241 atccgcctga cgggcggctc caaggaggac gcgatccatc aggccgagcg catcggcttc
    18301 gcattcatgc gcgagtacat ggaggcgcgc ggcctcacgc aggtggtcga tggttcgccc
    18361 gagaagacgc cagcggcact cgggcgcctg cagctccgcg agatcgagaa gtcggtgctt
    18421 ctccagacac tcgaccatct ctggcgcgag catctcgtga cgctcgagca tctccgccag
    18481 gtgatcggct tccgctccta tgcgcagcgc gatccactga acgagtacaa gtccgagagc
    18541 ttccagcatg ctcgcgcgtc tccgccagga cgtgaccggc cagctgatgc acatcgagtt
    18601 ctcggctgat ccgcaggcga cggaggtgcc ggcagctccc atgccgatgg cacctcccgc
    18661 ggacacgggc gaggcaccgg ccgcgccaac gcctgcgcgc gcaaatggcg cactcgcggc
    18721 gttcggtgtg cgcgaggagg agctcgccat ggccgacgcc gtgcttcgcg ccgagaagga
    18781 gccgcggccg gcgccgc
//
LOCUS       DAORNH010000049        18764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2260_length_18764_cov_2.855684, whole genome shotgun sequence.
ACCESSION   DAORNH010000049 DAORNH010000000
VERSION     DAORNH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18764
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_18764_cov_2.855684"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK264_07155"
     CDS             complement(<1..294)
                     /locus_tag="PK264_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75697.1"
                     /translation="MSVERVSGAVMIRGISGFVAVLLGSGLTWQSVHGVGTFVTDVDL
                     RELVQTFRVRLELAELIGRLDPVAPSAALLAEFRVLQARSKTLRDDPDPRRFAQ"
     gene            complement(355..1083)
                     /locus_tag="PK264_07160"
     CDS             complement(355..1083)
                     /locus_tag="PK264_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HRD75698.1"
                     /translation="MRDRPVPIHAEPSPTALDHQASMPRAASPRLIASEADVKAGLRA
                     LRRKCPAMRLAHDLAGDPPLRLREPGLEGLMRIIVAQQVSVASATAIWARVAAGVRPL
                     EAATILAMSEEQLRACGLSRPKIRTLTAIARAISEDGLDLGALADASEAEIHEQLTAV
                     SGIGPWTADIFAMFCLGRADAFAAGDLALQEAARLILELDERPKPDELGEIAERWRPW
                     RGVAARMLWQYYRVAKAASSGAPA"
     gene            1156..1581
                     /locus_tag="PK264_07165"
     CDS             1156..1581
                     /locus_tag="PK264_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HRD75699.1"
                     /translation="MITAVVLAEGHAAGPPIALAEPLSFWGGFDAASGRVIDRRHPDV
                     GRSLTGKILVMPAVRGSSSGSSVLAEAIRIGTAPLGIILGERDAILPVGAMVAAALYG
                     KSMPIVVVRPDELGVVAAAAAVEILAGPAARIVLTCVSA"
     gene            complement(1587..2768)
                     /locus_tag="PK264_07170"
     CDS             complement(1587..2768)
                     /locus_tag="PK264_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD75700.1"
                     /translation="MHDRYLRTGIFLAPFHARGENPTLAIERDMELVEHLDRLNYHEA
                     WIGEHHSGGFEIIACPEMFLAAAAVKTRNIRLGTGVVSLPYHHPFTLASRMMQLDHMT
                     RGRAMFGVGPGALIYDAEKIGLKAADQRRRMAESLDCIVELMHGRTVSRTTDWFTLDE
                     ARLQLASYSQPSMEMAVACSRSPVGAVASGKHGIGMLSIGGTSDDALKAHAANWAIHE
                     EAAAKAGKRANRSKWRIVTFAHVAETREKARADMAHGLADFCRYFTDVATFPIVPAGI
                     TDPTDYLVGSGLACIGTPDDCIRHFERLWQGSAGGFGAVLLLAHNWADWEATKRSYEL
                     MARYVHPQFQRQSNALRISSYHDAKLKHETAGAQSRDAVNAEIDKYNKARGITGSGKS
                     W"
     gene            complement(2802..3572)
                     /locus_tag="PK264_07175"
     CDS             complement(2802..3572)
                     /locus_tag="PK264_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75701.1"
                     /translation="MAGLLAGHIAAVTGGGSGIGQGICQAYAREGARVFVLDVNTEGA
                     AETVGLVTKAGGKASAMRLDVTDREACKAAAAEIAKGGNISVLVNNAGINRRNAFAGD
                     KAAVAKDWDDIMSINLDGIFNVTHAFLDQLRATKGRIVNIGSIQSFVHVNGWPNSAAY
                     TTSKHGVLGFTRALAAELGKDGVRVNAIGPGMIATRINAEARAKNPDMVAASMRHTPL
                     DRVGTPEDIAGPAVFLASDLSSYVTGHIIMADGGFRTV"
     gene            complement(3753..>4118)
                     /locus_tag="PK264_07180"
     CDS             complement(3753..>4118)
                     /locus_tag="PK264_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75702.1"
                     /translation="GKVYWTELNTHDVEKAKKFYAATLGWKFEAMPMPGFTYWIIKAG
                     DEVVGGMFELASPEFQGIPEHWLTYIAVDDVDKRAAKAVKAGGKLVRPAFDVPGVGRI
                     AIIMQPGGGGIGWMTPAPM"
     assembly_gap    4119..4130
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4270..>4700)
                     /gene="tsaA"
                     /locus_tag="PK264_07185"
     CDS             complement(4270..>4700)
                     /gene="tsaA"
                     /locus_tag="PK264_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRD75703.1"
                     /translation="PERTDARLVFIGRIRTPYATRADCPRQPDEGGPACRLEVEPTWQ
                     PALAGIKVGDRVEVIYWMHQARRDLVVMSPKRDGRTTGAFTLRSPARPNPIAISTVTV
                     ERVEPGALVVRSLDCVDGTPLLDIKPERCHFTPQAVTKSA"
     assembly_gap    4701..4712
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5323..5613)
                     /locus_tag="PK264_07190"
     CDS             complement(5323..5613)
                     /locus_tag="PK264_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75704.1"
                     /translation="MVEPESEPPAAIARQLVGARIVGGVEVSTINHADVAIPMGEAVL
                     VIKPPSRFNVYGKTFADVLGQHYAFTVADPAEGGFRQSPDDLCRVLDMVGSA"
     gene            complement(5890..6168)
                     /locus_tag="PK264_07195"
     CDS             complement(5890..6168)
                     /locus_tag="PK264_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75705.1"
                     /translation="MLSARPLTVRLRLRHARHAIHEEAAETVEIAPAIERHHPPAISL
                     PDKVYKLRQIADVASSHNQMEWLRAGRRRHAATTTYRTDDALVSDGRV"
     gene            6472..7377
                     /locus_tag="PK264_07200"
     CDS             6472..7377
                     /locus_tag="PK264_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD75706.1"
                     /translation="MAESEAEFIAVSNRKGGVGKSTVAIMLAHAAAAYGRRRVLVMDL
                     DTQCNASLMLIGGEAWDKARRAGKTIADYFFDRFDKVAEDESEYVVASAGDVAHPRTG
                     EGRVALLPGSLLLEDVQGELFLKQAKTGQDAELVASQVRTRMKRLMKHFGMDYDLVIM
                     DCPPGLSFAALAALDLADRVVVPFRPDFVSEFALDRASLLIEKVNSAEELAEIALAKR
                     RYACLPNYLRGTPRERLVLEQLALDHPMLTSRLPELASVARAFDWDMTKKTMEQKFGD
                     ALPHIRALHDELFGASRALGRRQAV"
     gene            7401..7844
                     /locus_tag="PK264_07205"
     CDS             7401..7844
                     /locus_tag="PK264_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75707.1"
                     /translation="MTKEMKTLSGAALDRYFRRVLRSEPALTPEIAERLAERLKANIA
                     SLKLRREAQSAEAADPAAAAVQGPVPGFDPYSPNLVVVVRLAGRDAALAALAAIESPD
                     DLRLLAREQQLSVEPSVATPAELRLAIVAAAEQRIANRRAAARGA"
     gene            complement(7869..8348)
                     /locus_tag="PK264_07210"
     CDS             complement(7869..8348)
                     /locus_tag="PK264_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75708.1"
                     /translation="MEAITPCLWFEGQAQEAVEFYTSVFPRSAITRVHRAMADTPGPK
                     SGDVLFIEFTLAGRSYQALNGGVHDKFNDAISLSVACEDQAEVDRLWAALTANGGRPV
                     ACGWLKDRYGVSWQIIPKRWFELIANADAEQGRRLMQAMMGMVKLDIATLEAVAKGP"
     gene            complement(8372..9286)
                     /locus_tag="PK264_07215"
     CDS             complement(8372..9286)
                     /locus_tag="PK264_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HRD75709.1"
                     /translation="MDLVTYLSFNGQCESAFRHYEKVLGGKILMMLKGSDAPSGTPVD
                     PQFRDRVMHARLEVDGRLLMGGDAPGGQDVRPQGFSAHIKIDDPGDAERIFRELSEGG
                     TVHVPLAEAFWARRFGMLTDRFGTPWMVNCEKPFGVPETEGTPFVISRTFEAPRPLLW
                     ACFTEEERMKRWWGPKGVRIAWSKIDFRPGGRHHYAMQLPDGKMMWGRQVYREISAPE
                     KIILVNSFSDEFGGLGRHPLAPSWPIELHTVFAFEEAGPAKTKFTVTWTPIYPTTEER
                     AAFDGGHASMTGGWGGTFDQLEEYLKTL"
     gene            9595..11028
                     /locus_tag="PK264_07220"
     CDS             9595..11028
                     /locus_tag="PK264_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HRD75710.1"
                     /translation="MRRGAMAYDEMHGVGRGKRCREPYREVATWLAGQSNDELVRKRR
                     EAEELFRRTGITFAVYGAKEADERLIPFDIIPRVISAAEWRRLSAGIEQRVRALNAFM
                     YDIYHRQEILRAGRIPEDLVIQNAAFLPEMMNVEPPRGIYSHIIGIDIVRTGENDFLV
                     LEDNTRTPSGVSYMLENREAMMYMAPDLFARNRVAPVERYPEDLRATLESVAPTGLDR
                     APTIVVLTPGQHNSAYFEHSFLADRMGVELVEAQDLVSTSDGVMMKTTEGLRLVDVVY
                     RRIDDAYLDPLAFKPDSVIGVPGIFEHYRAGRVTIVNAPGTGIADDKSIYCYVPEIIE
                     FYTGRPPILKNVHTYNCRKPADLAYVLEHLAELVVKEVHGSGGYGMLIGPTASREEIA
                     TFRARLEAEPARYIAQPTLALSTCPTLTSEGIAPRHLDLRPYVLLGDRMRLTPGGLTR
                     VALKKGSLVVNSSQGGGTKDTWVLDDA"
     gene            11052..11993
                     /locus_tag="PK264_07225"
     CDS             11052..11993
                     /locus_tag="PK264_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HRD75711.1"
                     /translation="MLGRTANDLFWMARYMERAENMARLLEVGHRLSLLPREEGQQEW
                     RSTLRSAGCEAGFLARNSSFEAAEVIDYLLFDKDNPSSVYSCIAAARRNARAQRTAIT
                     REMWESLNSAWIAYASRSSDQISSNALPALFDWVRERSALFRGALLNTILRNDTYYFT
                     QLGTFIERADNTARILDVKYYVLLPQNELVGGGLDSAQWAAILRSVSAHRSFRWVYRN
                     VFKPWHIADFLILNPQMPRSLRSCYAELVTVLDELAASYKSRTSCQELAAETLARITN
                     SDMPAIFQTGLHEYLLEFIASNTRLASEIATAYHFND"
     gene            12003..12812
                     /locus_tag="PK264_07230"
     CDS             12003..12812
                     /locus_tag="PK264_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRD75712.1"
                     /translation="MRLTIEHKTRYRYAEPATSSVQCLRLTPPSFVGQKVIDWRITSD
                     PGAPLRDGQDGFGNQLHLLVVRNRHQEIEVTAAGTIETGDRAGVVLGLPDTVPHRVYL
                     RRTDLTAATPEIMALADATPKSEFIPRLHCLMDAIRDHVDYLPGVTKAETTAGEAFDA
                     GRGVCQDHAHIFVAAARHLGLPARYVTGYLLTGSQSHDVAHHAWAETFVDGLGWLGFD
                     VANRVCPTDHYVRLAAGLDAHHVAPIRGSRRGGAGEKLSVEVNVAAAAAQQ"
     gene            12846..13580
                     /locus_tag="PK264_07235"
     CDS             12846..13580
                     /locus_tag="PK264_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRD75713.1"
                     /translation="MTYAVAVRLDRGLVFAADTRTNAGIDNVSQFRKLHFWRRTGDRV
                     LVLLTAGNLAVTQSVISMINEQLSAEAAGDTPTLMNVTSMYRAARLVGDAIREVRALD
                     GPSLEASKAGFSASFILGGQIGDEAPRLFQLYAEGNFIEATEDTPYFQIGEHKYGKPI
                     LDRVARGDMRLREAAKLILLSYDSTLRSNLSVGMPIDLLIYERDTLDVRRLRRIFESD
                     PYYQALSKGWADALRSAVAKMDVYDG"
     assembly_gap    13645..13654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13655..14636
                     /locus_tag="PK264_07240"
     CDS             <13655..14636
                     /locus_tag="PK264_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75714.1"
                     /translation="SGLAMMLCAALDAPAFARDPNGPPARVPQPDRSAVCGGETLPVV
                     HLRDNDGAAPYVKLRAGGISGHFLIDWGATASSVAARRYPSARERLEARDFSLPSFAS
                     GTFMLRDYALSLEPPGGQLGIVGTDFLSLLAAHLVYGDRGTTLSLSSTPCRAEGLRAG
                     GFRPISQKGFYSSDLSRIEKGRPNIPVLFLSLAGVVAPAQIDSGYEDITLRHSIDINT
                     PMHRALIAAGVRFRELEAITIATCTGTEQRAVLVPEGAAVAITDERGARIRDLDGAHL
                     ILKAPGSCGGIAGMAEPAAQIGASTLRTLREIVIDGKSETVWIRAGQPAR"
     gene            complement(14893..15207)
                     /locus_tag="PK264_07245"
     CDS             complement(14893..15207)
                     /locus_tag="PK264_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75715.1"
                     /translation="MRLFNDPFEAIDRDDGPTHLKSGIEFRDQPWRVGCDDRDVIDML
                     TCGRETALHLWAMVSIRLAWDPATVLRAHARHDLDIEGRDLRRPTPGKRSQYGLGKKR
                     AQ"
     gene            15385..16476
                     /locus_tag="PK264_07250"
     CDS             15385..16476
                     /locus_tag="PK264_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75716.1"
                     /translation="MLLGAAFSIVFASTSLAEPIEVIDAIGRKVVVNAPAKRVVINFN
                     FEEFTAVAGVEGWSRVVGMSRAPWESWRPVIFNRYKAVIPSLANIPDIGNSDEGTFSA
                     EKVIALKPDVFFLAAWMFNALKGADKQIEAAGIPLVVIDYNAQKLESHLASTRLLGKI
                     MGTEARAEELARFYETKYQDVMARVERASATKRPRVYLELGQGGAEVYGNTYDKGLWG
                     LIFALMKADNIAAGKVPGAYGPINAEAVIAANPEVIFIAGSSWVNRPKAVRTGYDTAP
                     DVTRASLAPYAARPGWADLAAVKSGNISAMEHGLARSLFDFVAMQYIGKRLYPEAFAD
                     IDPHAEFKAYHERFLPVPFNGTWVLPLRP"
     gene            16473..17516
                     /locus_tag="PK264_07255"
     CDS             16473..17516
                     /locus_tag="PK264_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRD75717.1"
                     /translation="MSKPTSVAILYTRLAARRRRVLVVAGLSLVASVLVDVATGPAFL
                     PVANVARSILGANSDRTVDTIVWTIRLPIAAMALVVGASLGLTGAVMQTILNNPLASS
                     YTLGVSAGAGFGAALVIVLGSVVPVSEQFAIPASAFLFAGVACTGVWLVGSRRGATPE
                     MLVLGGIAFLFLFQALLSLLQFIASQEALQQIVFWLFGSLQRATWGKFSIVATVLIVS
                     IPVLAADAWRLTALKLGDDRARGLGVDVTSLRKRCFVLVSLLTGVAVAFVGTIGFVGL
                     VGPHVARMLVGEDQRTFLPASALFGALLLSLASIASKTIVPGAIFPIGIVTALIGVPF
                     FIWLVISNKRAYW"
     gene            17513..18283
                     /locus_tag="PK264_07260"
     CDS             17513..18283
                     /locus_tag="PK264_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75718.1"
                     /translation="MIVEARGLSVHYGDVTAVDGIDLTVTPGTLLALVGPNGSGKSSL
                     VKAIAGVVRAEGSLQFGGRPHRPGTVAFMAQDIGGQAALSVLEVVLLGRLRRLGLSIS
                     SGDTAAVGAVMDELDLTHLAGRHLGELSGGQRQMVFLAQALASDPQVLLLDEPISALD
                     LRHQLDVLDLVQRHTKTRGLATVCVLHDLTAAARIADRVAMLRRGRLVAEGPSARILT
                     ADLLADVFEVEAEILTTRAGLPVVSPTRTRSRSDPSSQ"
     gene            18479..>18764
                     /locus_tag="PK264_07265"
     CDS             18479..>18764
                     /locus_tag="PK264_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRD75719.1"
                     /translation="MARTVAEELGAYAAAELARPLAPEIEHHARRALIDWFAALFPGT
                     LISPARELFTALDDEIGHGGAWVYGLDRRAPIRTAALVNGAVSHAVEFDDI"
BASE COUNT         3079 a         6241 c         6076 g         3334 t
ORIGIN      
        1 ctgggcgaag cggcgcggat cgggatcgtc gcgcaatgtc ttcgaccgcg cctgcagcac
       61 acggaattcg gcaagcagcg ccgccgacgg tgcaaccggg tccagccgtc cgatcagttc
      121 ggcaagctcg agccggacgc ggaaggtctg caccagttcc cgcaggtcca cgtcggtgac
      181 gaaggtgccg acgccgtgca ccgattgcca cgtgaggcca gagccgagca gcaccgccac
      241 aaagcctgag atcccccgta tcatgaccgc ccccgagacc cgctcgaccg acatgcccgc
      301 tgccggtgac gaggatagca gaccgcggcc tcagctgtcg ccgctcgccg tttctcaggc
      361 gggcgccccc gaggacgcgg ccttggcgac ccggtaatac tgccagagca tgcgggccgc
      421 gaccccgcgc catggccgcc agcgttcggc gatctcgccg agctcgtcgg gcttcgggcg
      481 ctcatcaagc tcgagaatga gccgcgccgc ttcctgtagg gcgagatcgc cagccgcaaa
      541 ggcatcggcc cgcccgaggc agaacatggc gaagatgtcg gcggtccagg gcccgatgcc
      601 actcaccgcc gtcagctgct cgtggatctc agcctcgctc gcgtccgcaa gcgctccgag
      661 gtcgaggccg tcttcactga tggctcgggc gatggccgtc agtgtcctga ttttcggtcg
      721 cgagaggccg caagctctga gctgctcctc gctcatggcg aggatggtag ccgcctcgag
      781 cggtctcaca ccggcggcga cgcgcgccca gatcgcagtg gcactggcga ccgagacctg
      841 ctgggcgacg atgatccgca tgagcccctc gaggcccggc tcgcggagcc gcagcggtgg
      901 atcgccagcg aggtcgtgcg cgagacgcat ggccggacat ttgcgcctga gcgcgcgcag
      961 gccggccttg acgtcggcct cggacgcgat cagtcggggg gaagcggctc gcggcatgga
     1021 cgcctgatga tcgagtgcgg tcggtgacgg ctcggcatgg attggcacgg ggcggtcgcg
     1081 catgcaattg tcgctggacg gccggtgtga cgacatcgcg ccccatcctg cgctacccaa
     1141 catgttcgaa acgccatgat caccgccgtc gtgctcgccg aaggccatgc cgcggggccg
     1201 cctatcgcgc ttgcggagcc gctgagtttc tggggcggat tcgacgctgc gagcggccgt
     1261 gtcatcgacc gccgccaccc cgacgtcggg cgctccctca cggggaagat cctggtgatg
     1321 ccggccgtgc gtgggtcatc ctccgggagc tccgtgctcg ccgaggcgat ccgcatcggc
     1381 acagcaccgc tcggcatcat cctcggcgag cgcgatgcca tccttcccgt cggcgccatg
     1441 gtggccgcgg cgctttacgg caagtcgatg ccgatcgtcg tcgtccggcc cgacgagctt
     1501 ggcgtcgtcg cggccgccgc tgcggtcgaa atcctggccg ggccggcggc gcgcatcgtg
     1561 ctcacgtgtg tgtccgcctg aggctgctac caggatttcc ccgagcccgt gatgccgcgc
     1621 gccttgttgt acttgtcgat ctcggcgttg acggcatcgc gcgattgcgc gccggccgtc
     1681 tcgtgcttca acttggcgtc gtggtagctc gatatcctca aagcgttcga ctggcgctgg
     1741 aactgcgggt gcacgtagcg cgccatcagc tcgtagctgc gcttcgtcgc ctcccagtcg
     1801 gcccagttgt gcgcgagcag cagcaccgcg ccgaagccgc ccgcggagcc ctgccagagt
     1861 cgctcgaaat ggcggatgca gtcatcgggc gtaccgatgc aggcgaggcc actgccgacg
     1921 agatagtcgg tcggatcggt gatcccggcc ggaacgatcg ggaacgtggc gacatcggtg
     1981 aagtagcggc agaagtcggc gaggccatgc gccatgtcgg cccgcgcctt ctcgcgtgtc
     2041 tcggcgacgt gcgcaaaggt cacgatccgc catttcgaac gattagcccg cttgccggcc
     2101 ttggccgctg cctcctcgtg gatcgcccag ttggcggcat gcgccttcaa ggcatcgtcg
     2161 gacgtaccgc cgatcgacag catgccgatg ccgtgcttcc ccgaggcaac tgctccgacc
     2221 ggcgagcgcg agcaggcgac cgccatttcc atggacggct gggaatagga ggcgagttgc
     2281 agccgcgcct cgtcgagcgt gaaccagtcc gtggttctgc tgaccgttcg tccgtgcatc
     2341 agctcgacga tgcagtcgag ggattcggcc atacgacggc gctggtcggc ggccttgagg
     2401 ccgatcttct ctgcatcata gatgagggcg cccggcccga cgccgaacat cgcccgcccg
     2461 cgcgtcatat ggtcgagttg catcatgcgc gaggcgagcg tgaaggggtg atggtagggg
     2521 agcgacacga cgccggtgcc gagccggata ttgcgcgtct tgaccgccgc cgccgcgagg
     2581 aacatctcgg ggcaggcgat gatctcgaag ccgcccgagt ggtgctcgcc gatccaggcc
     2641 tcgtggtagt tgagccggtc gaggtgctcg acgagctcca tgtcgcgctc gatggcgagc
     2701 gtcgggttct cgccgcgcgc atgaaacggc gccaggaaga tcccggtccg caagtagcgg
     2761 tcatgcatgg gtgtgtcctg tgattggtcg cgcagccgtc gtcagacggt acggaagccg
     2821 ccgtcggcca tgatgatgtg accggtcaca taggacgaca ggtccgaggc gaggaacacg
     2881 gcagggcctg cgatatcctc cggcgtgccg acgcgatcca gcggcgtgtg gcgcatgctg
     2941 gcggcgacca tgtcggggtt cttggcccgt gcctcggcat tgatgcgcgt tgcgatcatg
     3001 cccgggccga tggcattgac acgcacgcca tccttgccga gctcggcggc gagcgcccgc
     3061 gtgaagccga gcacgccgtg cttcgacgtc gtgtaggccg ccgagttcgg ccagccgttg
     3121 acgtgcacga acgactgaat cgagccgatg ttgacgatcc ggcccttggt cgcgcgcagc
     3181 tgatcgagga aggcgtgcgt gacgttgaag atgccgtcga ggttgatcga catgatgtcg
     3241 tcccagtcct tggcgacggc ggccttgtcg cccgcgaacg cattgcgccg gttgatgccg
     3301 gcattgttga cgagaaccga aatgttgccg cctttggcga tctcggcggc ggctgccttg
     3361 caagcctcac gatcggtaac gtccagccgc atggccgagg ccttgccgcc ggccttggtc
     3421 acgagaccga cggtttccgc cgcgccctct gtattgacgt cgagcacgaa tacgcgcgcg
     3481 ccttcccgcg cataggcctg gcagatccct tggccgatgc ccgaaccgcc gcccgtcacc
     3541 gccgcgatgt gtcctgcaag aagccctgcc atggtatcct ccgggtgagt tgcgtgctca
     3601 gtcggactgc gagcccgtcc tgcgcgctct tgtcgaaggc gaggctagcg cggggcggcg
     3661 ggcgttgcca gcaaggaagt accgcgcgtc atagatgcct gcggcagcgc cggcgcattg
     3721 ctcacggttc gctgtcggct cacccgcaag tctcacatgg gcgcgggagt catccagcca
     3781 atcccgccgc cgccgggctg catgatgatg gcgatccggc cgacaccagg gacgtcgaat
     3841 gcggggcgca cgagcttgcc cccggccttc accgccttcg ccgcgcgctt gtcgacatcg
     3901 tcgacggcaa tataggtcag ccaatgctcg ggtatgcctt gaaactcagg actcgcgagc
     3961 tcgaacatgc cgccgaccac ttcatcgcca gccttaatga tccagtacgt gaagcccggc
     4021 atcggcatcg cctcgaattt ccagccgagg gtcgccgcat agaacttctt cgccttctcg
     4081 acgtcgtgcg tgttgagctc ggtccagtag accttgccnn nnnnnnnnnn aggcaggcgc
     4141 cgccggctcc tgcttcggtg acggcttggt ggcctttgga gatttcgcgg cactcttcgg
     4201 cttcgttgcc atggctcgct cctcgtttcg atgcgcgaat ggtcggcggg tcgatacact
     4261 caaccagcat catgccgact tcgtgacggc ctgcggcgtg aagtggcagc gttccggctt
     4321 gatgtcgaga agcggcgtgc cgtccacgca atcgaggctc cgcaccacca gcgccccggg
     4381 ctcgacgcgc tcgactgtca ccgtcgagat ggcgatcggg ttcgggcgcg caggcgagcg
     4441 cagcgtgaac gcgccggtgg tgcgcccgtc gcgcttcggc gacatgacaa cgaggtcgcg
     4501 ccgcgcctgg tgcatccagt agatcacctc gacgcgatca cccaccttga tgccggcgag
     4561 cgccggctgc caggtcggct cgacctcgag ccggcaggcg ggaccgccct cgtccggctg
     4621 gcgcggacag tcggcgcgtg tcgcgtacgg cgtgcggatg cggccgatga agacgaggcg
     4681 ggcgtccgtc cgctccggca nnnnnnnnnn nnctcgcccg gccgcgtttc atcgtgtgac
     4741 atggctcagt ttccttcggc ggagccccat agcctacatg acccggcacc atgcgagcca
     4801 gtgggagccg agccatcggt ccggctgccg gcatcctcat gctgcaacgc cgcaattgct
     4861 gctcgcgacc cgcactgccg gaacctccca atgcactccg catgcccttg atatcaatag
     4921 caccatcaat gatatcgcga gatcttaagc ggccttttca acccacagtc acaacggtat
     4981 gtctgataga gaaaaattac ttggcgagga gttgggcatt cggtccatga gggcgatcaa
     5041 gcagcaggtc tgagccgctg aattctgcaa gacggaggtg cgcattgcgt cttcaagaat
     5101 gttcccgaag aactgatcgc tgtgacaggg gcggcgtttg tgcgtggctc gcgcatcgcg
     5161 aacgctgagg caccaggggg cagaagatgg tcgatcacgc gagcctcgga tacgccattg
     5221 ctcatcccac aatcgatcag gcggcccgta gccgtcgtcc gtggcgcgtc acgcgtcaaa
     5281 cgtgcctccc ctccatcacg cattactcgc ggctggcctg gactaggcac tcccgaccat
     5341 gtcgagcacg cggcagagat catcgggcga ctgacggaac cctccttcgg ccgggtcggc
     5401 aaccgtgaac gcatagtgtt gcccgagcac gtcggcaaag gtcttgccgt agacattgaa
     5461 gcgtgacggc ggcttgatga cgagcacagc ctcgcccatc ggtatggcga cgtcggcatg
     5521 attgatcgtc gagacttcga cgccgccgac gatcctcgcg ccgacgagct gtcgcgcaat
     5581 tgcggccggc ggctcgctct cgggctcgac cacgataaag ccctgtgccg atagctattc
     5641 gatgatctcc ggctcgttca cgatccgcct tggtgccccg ctcgttccag gttggatgaa
     5701 taccggtcgt gccgagtcgc ttggcgcgga cttccggatg agctcgcgaa ggacgtcgat
     5761 caggaaatgt ccgaagtagt tttcggccga ctcggtggtg ctgagctggc caaggtcgcg
     5821 ctctgcaaac gcgggcggag cgaagagctt gcgccgatgc gtgtgatact cctcgtaggc
     5881 accctagcct tagacacgac cgtcggaaac cagcgcgtcg tcggtgcggt aggtcgttgt
     5941 cgccgcatgc cggcgccggc ccgccctcag ccactccatc tggttatgac tgctggcgac
     6001 gtcggcgatc tggcggagct tgtagacctt gtccggcagc gagatcgccg gtggatggtg
     6061 ccgctcgatc gcgggggcaa tctcgaccgt ctcggccgcc tcctcgtgga tcgcgtggcg
     6121 cgcgtgccgc agccgcaagc gaaccgtcag cggtctcgca ctcaacatca ggtcggttcc
     6181 ctcggcaaaa agacggcgca tgcccgagca cactgtcgac ccttgcctcg cgatgagagg
     6241 cgggcggcgc aagccgctga agagacgagg cgatgacgcg ctcagtgcca ggggtgacac
     6301 cggcatctgc atggacatgc aatcctttgc tcatgtcacg tcagataggt tgggccgctc
     6361 cggcggggcg cgaccgtcgt gacgccccat tgaaccggcg ccgctcgacc aacaggacaa
     6421 gcgcccgcga atcctataac cggccgtgtc acagcatcga gcgaggtcgt catggcggag
     6481 tctgaggcgg agttcatcgc ggtctcgaat cgcaagggcg gagtcggcaa gtcgacggtt
     6541 gccatcatgc ttgcgcatgc cgccgcggca tatgggcgcc gccgcgtcct cgtcatggac
     6601 ctcgacacgc agtgcaacgc ctcgctgatg ctgatcggcg gcgaggcatg ggacaaggcg
     6661 cggcgcgccg gcaagacgat cgccgattac ttcttcgacc gcttcgataa ggtcgccgag
     6721 gacgagagcg agtatgtggt cgcgagcgcc ggcgatgtcg cccacccgcg caccggcgag
     6781 ggtcgcgtcg cgctgttgcc gggctcgctc ctgctcgagg acgtgcaggg cgagctcttc
     6841 ctgaagcagg cgaagaccgg ccaggatgcc gagctcgtcg cctcgcaggt ccgcacccgc
     6901 atgaagcggc tcatgaagca cttcggcatg gattacgacc ttgtcatcat ggattgcccc
     6961 ccggggctct ccttcgcggc gcttgccgcc ctcgacctcg ctgaccgcgt ggtggtgccg
     7021 ttccgaccgg atttcgtctc cgagttcgcg ctcgaccgcg cttcgctgct gatcgagaag
     7081 gtcaactcgg ccgaggagct tgccgagatc gcgcttgcga agcgccgcta tgcctgcctg
     7141 ccgaactatc tgcgcggcac gccgcgcgag cgcctcgtcc tcgagcagct cgccctcgac
     7201 catccgatgc tgacgtcgcg cctgcccgag ctagcgagtg tcgcgcgcgc cttcgactgg
     7261 gatatgacga agaagacgat ggaacagaag ttcggcgacg cgctcccgca cattcgcgcg
     7321 ctgcacgacg agctgtttgg ggcgagccgc gccctcgggc ggcgccaggc ggtctgaagg
     7381 gagcgggagg aggagcggtc atgaccaagg aaatgaagac gctctccggc gccgcgctcg
     7441 accgctattt ccgtcgcgtc ctgaggtcag agccagcgct gacgcccgag atcgcggaac
     7501 gcttggcaga gcgcctcaag gccaatatcg cgtcgctgaa gctgcgccgc gaggctcagt
     7561 cggccgaagc agcagacccc gcggcggcag cggtgcaggg ccccgtgccg ggtttcgatc
     7621 cctattcgcc gaacctcgtc gtcgttgtgc gtctggcggg acgcgatgcc gcgcttgccg
     7681 cgctcgccgc catcgagagc ccggacgacc tgcgcctcct cgcccgcgag cagcagctct
     7741 cggtagaacc gagcgttgcc acgcctgccg agctccgctt ggccatcgtc gccgccgccg
     7801 agcagcgcat cgccaaccgc cgcgccgccg cacgaggggc ctgagccacg tcgctcacgt
     7861 cgttcggctc acgggccctt tgccaccgcc tccagcgtgg cgatatcgag tttcaccatg
     7921 cccatcatcg cctgcatgag gcgccggccc tgttcggcgt cggcattggc gatgagctcg
     7981 aaccagcgct tcgggatgat ctgccacgag acgccgtacc tgtccttgag ccagccacag
     8041 gcgaccggac ggccaccatt ggcagtcaag gctgcccaga gacgatcgac ctccgcctgg
     8101 tcctcgcagg cgacggagag cgagattgca tcgttgaact tgtcgtgcac gccgccgttc
     8161 aacgcctgat agctccgtcc cgccaacgtg aactcgatga agagcacgtc gccggacttc
     8221 gggccgggtg tatcggccat ggcgcggtgc actctcgtga tagcgcttcg cgggaatacg
     8281 gaggtgtaga attctacggc ctcttgcgcc tggccttcga accagaggca gggtgtgatc
     8341 gcttccatga tcgggtctcc gtgatccggc gtcagagcgt tttcagatac tcttcgagct
     8401 gatcgaaggt gccgccccac ccgccggtca tgctggcgtg gccgccatcg aaagccgcgc
     8461 gctcctccgt cgtcgggtag atcggcgtcc aggtgacggt gaacttcgtc ttggccgggc
     8521 ctgcctcctc gaatgcgaag accgtgtgca gttcgatcgg ccaggagggc gcgagcggat
     8581 ggcgcccgag cccgccgaac tcgtccgaga acgagttgac gaggatgatc ttctcgggcg
     8641 cggagatctc gcgatagacc tgccggcccc acatcatctt tccgtcgggg agctgcatcg
     8701 catagtgatg gcggccgccg ggcctgaaat cgatcttcga ccaggcgatc ctcacgccct
     8761 tcggccccca ccaccttttc atccgctcct cttcggtgaa gcacgcccag aggagaggac
     8821 gcggcgcctc gaaggtccgc gagatgacga acggcgtgcc ctccgtctca gggacgccga
     8881 acggcttctc gcaattgacc atccagggcg ttccgaagcg gtcggtgagc atgccgaaac
     8941 ggcgcgccca gaaggcttcg gcaagcggca cgtgcaccgt cccgccctcg ctgagctcgc
     9001 ggaagatgcg ctccgcgtcg cccggatcgt cgatcttgat gtgagccgag aagccctgcg
     9061 gcctcacatc ctggccgccc ggcgcgtcgc cgcccatcag cagtcggccg tcgacttcga
     9121 ggcgggcgtg catcacgcga tccctgaact gcgggtcgac cggcgtgccc gatggcgcgt
     9181 ccgatccctt cagcatcatc aggattttgc cgccgagcac cttctcgtag tggcggaacg
     9241 cggactcgca ctggccgttg aaggagaggt aggtgacgag atccatgggg ttgctcctcg
     9301 aactgggtga ttgaggcatc gcactgtcta gcagttccac cggcatctgt gggcactgaa
     9361 cagcaagtcg ggcagccagc gccggccggg gctgcttcgc gcgatcggcc cgccaaatgc
     9421 gtccgtcctg gatcaatcgg tgcgtccaat caggcgcggg gccctgcaag tccggaaggc
     9481 aataaggcac gttttcgccc aacaatcagg catccgaggg gacggttgtg gcgttactct
     9541 ggtgcagttg gccgaattca atgcaaggat aggatgaccg tccgaagtaa agacatgcgc
     9601 aggggcgcca tggcctacga cgagatgcac ggggtcggcc gcggcaagcg gtgccgcgag
     9661 ccctacaggg aggttgcgac gtggctcgcc ggccagtcga atgacgaact cgtccgaaag
     9721 cggcgagagg ctgaggagtt gttccggcgc acgggcatca cctttgcggt ctatggcgcc
     9781 aaggaggcgg acgagcgcct cattcccttc gacatcattc cgcgcgtgat ctcggccgca
     9841 gagtggcgcc gcctctccgc cggcatcgag cagcgcgtgc gtgcgctcaa tgccttcatg
     9901 tacgacatct accaccgcca ggagatcctg cgcgccgggc gcatccccga ggatctcgtc
     9961 atccagaatg ccgcgttcct ccccgagatg atgaacgtcg agccgccgcg cggcatctac
    10021 tctcacatca tcggcatcga catcgtcagg actggcgaga acgactttct ggtgctcgag
    10081 gacaacacgc gcactccgtc gggcgtctcc tacatgctcg agaaccggga agcgatgatg
    10141 tacatggcgc ccgatctctt cgcgcgcaat cgcgtggcgc cggtcgagcg ctatccggag
    10201 gatctgcgcg cgacgctcga aagtgtcgcg ccgaccggcc tagaccgcgc gccgaccatc
    10261 gtcgtgctca cgcccggcca gcacaacagc gcctatttcg agcactcgtt cctggccgat
    10321 cggatgggcg tcgagctcgt cgaggcgcag gacctcgtct cgacgtcgga cggcgtgatg
    10381 atgaagacga ccgagggcct ccgcctcgtc gacgtcgtct atcgccggat cgatgacgcc
    10441 tatctcgatc cgctcgcctt caagccggat agcgtcatcg gggtccccgg catcttcgag
    10501 cattaccggg cggggcgcgt cacaatcgtc aatgcgcccg gcacaggcat cgccgacgac
    10561 aaatcgatct actgctatgt gccggagatc atcgagttct atacgggccg tccgccgatc
    10621 ctgaagaacg tgcacaccta caactgccgg aagcccgctg atctcgccta cgtgctcgag
    10681 catctcgcgg agctggtcgt caaggaggtg cacggctcgg gcggctacgg catgctgatc
    10741 gggccgacgg catcccgtga ggagatcgcg acgttccgcg cgcgcctgga agccgagccc
    10801 gcacgctaca tcgcgcagcc gacgctcgcc ctctcgacat gtccgacgct gacgagcgag
    10861 ggcatagcgc cgcgccatct cgacctccgg ccgtacgtgc ttctcggcga ccgcatgcgg
    10921 ctcacgccgg gaggcctcac gcgcgttgcc ctcaagaagg ggtcgctggt cgtcaactcg
    10981 agccagggag gtggcacgaa ggatacctgg gtcctcgacg acgcataagg aggcgcatcg
    11041 cgcgatacgg catgcttgga cggacggcga acgatctctt ctggatggca cgctacatgg
    11101 agcgggccga gaacatggct aggctgctcg aggtcgggca tcgcctgagt ctgctgccgc
    11161 gcgaggaggg ccagcaggag tggcgctcga cgctgcggag cgccggctgc gaggcgggct
    11221 ttctcgcgcg caattcttcg ttcgaggcgg cggaggtcat cgactacttg ctgttcgaca
    11281 aggacaaccc ctccagcgtc tattcctgca tcgcggccgc acggcgcaat gcccgcgcgc
    11341 agcgcacagc gatcacgcgc gagatgtggg agagcctcaa cagcgcctgg atcgcctacg
    11401 cctcgcggtc atcggaccag atctcgtcca acgcgctgcc ggcactgttc gattgggtcc
    11461 gcgagcgttc ggcactcttc cgcggtgcgc tcctcaacac gatcctgcgc aacgacacct
    11521 actatttcac gcagctcggc accttcatcg agcgcgccga caatacggcc cgcatcctcg
    11581 acgtgaagta ctacgtgctg ctcccgcaga acgagcttgt cggcggcggc ctcgacagcg
    11641 cccagtgggc ggcgatcctg cggtcggtct ccgctcaccg cagcttccgc tgggtctacc
    11701 gcaacgtctt caagccgtgg cacatcgccg acttcctgat cctgaacccg cagatgccgc
    11761 gttcgctgcg ctcctgctat gccgagctcg tcacggtgct cgacgagctt gccgcctcct
    11821 acaagtcgcg gacctcgtgc caggagctcg ccgccgagac gcttgcccgt atcacgaaca
    11881 gcgatatgcc tgcgatcttc cagaccgggc tgcacgagta tcttctcgag ttcatcgcct
    11941 ccaacacgcg cctcgcctcc gagatcgcga ccgcatacca cttcaacgac tgagcgggtc
    12001 ccatgcgcct gaccatcgag cacaagacgc gctatcgcta cgcggagccc gccacctcga
    12061 gcgtccagtg ccttcgcctg acgccgccga gcttcgtcgg tcagaaggtg atcgattggc
    12121 gcatcacgag cgatccgggg gccccgttgc gcgatggcca ggacggcttt ggcaatcagc
    12181 tgcatcttct cgtcgtccgc aaccgccacc aagagatcga ggtgacggcc gctggcacca
    12241 tcgagaccgg ggacagggcc ggcgtcgtgc tcgggctccc ggatacggtg ccgcaccgcg
    12301 tctatctgcg ccggaccgac ctcaccgcgg cgacgcccga gatcatggcg ctcgccgatg
    12361 cgacgccgaa gagcgaattc atcccgcgcc tgcactgtct catggatgcc attcgtgatc
    12421 atgtggacta cctcccgggg gttaccaagg ccgagacgac ggctggcgaa gccttcgacg
    12481 cagggcgggg agtttgccag gatcatgccc acatcttcgt ggcggcggcc cgtcatctcg
    12541 ggctcccggc gcgttacgtg acaggctacc tgctcacggg ctcgcaatcg cacgatgtcg
    12601 cccaccatgc ctgggctgag accttcgtcg acggcctcgg ctggctcggc ttcgacgtcg
    12661 ccaatcgggt gtgcccgacg gaccactacg tgcgcctcgc tgctggcctc gacgcgcatc
    12721 atgtggcacc gatccgtgga tcgcgacgag gtggcgcagg tgaaaaactc tcagtcgagg
    12781 tcaatgtcgc ggccgcggcg gcgcagcagt agtggccgcg ctgacacgcc aggacgggag
    12841 ccccgatgac ctacgccgtt gccgtgcgcc tcgatcgagg gctcgtcttt gccgccgata
    12901 cgcgcaccaa tgctggcatc gacaacgtct cgcagttccg caagctgcac ttctggcgcc
    12961 gcaccggcga ccgcgtcctc gtgctgctga cggcggggaa cctcgccgtc acgcagtccg
    13021 tcatcagcat gatcaacgag cagctttccg ccgaggcggc aggcgatacg ccaacgctca
    13081 tgaatgtgac gtcgatgtac cgcgcagcgc gcctcgtcgg ggatgccatc cgtgaggtgc
    13141 gcgccctcga tggcccctcg ctcgaggcga gcaaagcggg cttctccgcc tccttcatcc
    13201 tcggcgggca gatcggcgac gaggcgccgc gcctcttcca gctctacgcg gaaggcaact
    13261 tcatcgaggc gacggaggat acgccgtact ttcagatcgg cgagcacaag tacgggaagc
    13321 ccatcctcga tcgcgtggca cgaggcgaca tgcggcttcg cgaggctgcg aaactcattc
    13381 tgctgtcata cgattccaca ctcaggtcga acctctccgt cggcatgccg attgatctcc
    13441 tgatctacga gcgcgatacg ctcgatgtgc gccgtctgcg ccgcatcttc gagtccgatc
    13501 cctattatca ggcgctgtcc aaaggctggg ccgacgcgct ccgctcggcg gtcgccaaga
    13561 tggatgtcta cgatggctga gatcggcaca cgatcgatgc gcctgtggtt cgcggcgcgg
    13621 ctcgcctcgg gattgcgcca atagnnnnnn nnnnctcggg ccttgcgatg atgctgtgcg
    13681 cagcacttga cgcgcccgcg ttcgcccgcg accccaacgg acctccggca cgtgtgcctc
    13741 agccggacag gtcggcggta tgcggcggcg agacgcttcc cgtggtccac ctccgcgaca
    13801 acgacggcgc cgcgccctac gtgaagctcc gcgccggtgg catctccggc catttcctca
    13861 tcgactgggg agcgacggcg agttcggttg cggcgcggcg ctatccgagc gcgcgtgaac
    13921 gcctcgaagc gcgcgacttc tcgctgccga gcttcgcgag cggcacgttc atgctccgcg
    13981 actacgcgct ctcgctcgag ccgccgggtg ggcagctcgg aatcgtcggc accgatttcc
    14041 tgtcgctgct cgcggcccac ctcgtctatg gggaccgggg caccacactc tcgctttcgt
    14101 cgacgccctg cagggccgag gggctcagag caggtgggtt ccgcccgatc agccagaaag
    14161 gcttctactc gtccgatctc tcgcgcatcg agaagggccg gccgaacatc ccggtgctct
    14221 tcctctcgct tgccggcgtc gtcgcgccgg cccagatcga cagcggctac gaggacatca
    14281 cgctgcgcca ctcgatcgac atcaacacgc cgatgcaccg cgcactcatc gcggcgggcg
    14341 tccgcttccg cgagctcgag gcgatcacca tcgcgacctg caccggcacg gagcagcgag
    14401 ccgtgctcgt tccagagggt gccgcagttg cgatcaccga cgagcgcggc gcgcgcatcc
    14461 gcgatctcga cggcgcccac ctcatcctca aagcgcccgg cagctgcggc ggcatcgccg
    14521 gcatggcgga gcccgcggcc cagatcggcg catcgacgct ccgcacgctc cgcgaaatcg
    14581 tcatcgacgg caagagcgag acggtctgga tcagggccgg acagccggcg cgatgacggg
    14641 gcggttccaa tgcgcccggg tgggcatcgc gccaggctct accatctatg gtctctgctc
    14701 cgcggcaccg aaagtagtca gcttgggggc caggagctgc cgagcgtaca gatttaaacg
    14761 gtgcgttgtt gatggtcggc tatgtggtac gatagcagaa taggttccaa tcaatcggcc
    14821 agcgacaagc ccgtgacccg cagattgcta acccgccaac attcaaacgc gcgccccatt
    14881 acggtggcga tgttactgtg cgcgcttctt gcccagaccg tactgggacc gcttgcccgg
    14941 agttgggcgg cgcaggtcac ggccttcgat gtcgagatca tggcgagcat gtgctcgcag
    15001 cacggtggcg gggtcccaag caagccggat cgagaccatt gcccacagat gcagtgctgt
    15061 ctcgcgtccg cacgtcagca tgtcgatgac gtcgcggtca tcgcaaccga cacgccatgg
    15121 ctggtcgcga aactcgatgc cggacttgag atgagttggc ccgtcatcac ggtcaatcgc
    15181 ctcgaacgga tcgttgaaaa gccgcaacag gcccgcgcac ctcccgcctg acacgtccgc
    15241 aactgcctga gccaaacctc ccgacgcctg cagcttgcgc gtcgcagccg actgcctgtc
    15301 atgccttgac gggggcacgg cagcgcgtgt gcgggcagat gccaagcaat ccacaggaat
    15361 cgtcagatgc aactttcgca cagcatgctg ctgggcgcgg cattctccat tgtcttcgcc
    15421 agcaccagct tggctgaacc aatagaggtt atcgacgcca ttggccgcaa ggtcgttgtc
    15481 aacgctcccg ccaagcgcgt ggtgatcaac ttcaacttcg aggagttcac agccgtcgcc
    15541 ggcgtcgagg gctggtcgcg ggttgtcggc atgtcccgcg caccttggga atcgtggcgg
    15601 ccggtgatct tcaatcgcta caaggcggtg atcccgagcc tcgccaatat acccgatatc
    15661 ggcaactccg acgaaggcac gttcagcgcc gagaaggtaa ttgcgctgaa gcccgacgtg
    15721 tttttcctcg cagcttggat gttcaacgcg ctgaaaggtg cggacaaaca gatcgaggct
    15781 gccggtatcc cgctcgtggt catcgactac aacgcccaaa aactggagag ccatctcgcc
    15841 tcgacccgct tgttgggcaa gatcatgggg accgaggcgc gcgccgagga attggcgcgc
    15901 ttctacgaaa caaaatatca ggacgtgatg gctcgggtcg aaagggccag cgcaacgaag
    15961 cgtccccgcg tctacctcga actcggccag ggcggcgccg aagtgtacgg caacacatac
    16021 gacaagggcc tttggggtct gatcttcgcc ctcatgaagg ccgacaacat cgcagcgggc
    16081 aaggtccccg gcgcctatgg tccgatcaat gcggaggcgg tgatcgcggc caatcccgag
    16141 gtgatcttca tcgccggctc gtcgtgggtc aaccggccga aggcagtgcg aaccggttac
    16201 gacacggcac ctgatgtgac gcgcgcctcg ctcgccccct atgcggcgcg ccccggctgg
    16261 gccgatctgg cggcggtcaa gtcgggcaac atcagcgcga tggagcacgg attggcgcga
    16321 agcctgttcg acttcgtggc catgcagtac atcggcaagc ggctctatcc agaggcgttc
    16381 gctgacatcg acccgcacgc cgagttcaag gcctatcacg agcggttcct cccggtgccg
    16441 ttcaatggca cctgggtact gccgttgcgg ccatgagcaa gccaacttcg gtcgcgatac
    16501 tctacacccg cctcgctgcc cgccgccgcc gcgttctggt ggtggcgggc ctcagccttg
    16561 ttgccagtgt cttggtcgac gttgcgaccg ggccggcgtt cctgccggtt gccaacgttg
    16621 cgcgttcgat cctgggcgca aacagcgatc gcacggtcga caccatcgtg tggacgatcc
    16681 gtttgccgat cgccgccatg gcgctggtcg tgggggcgtc gctcgggctg acaggcgcgg
    16741 tcatgcagac tatcctcaat aaccctctgg cctcgagcta cactctcggg gtttcggccg
    16801 gtgccgggtt cggcgcagcg ctcgtcatcg tgctcggaag cgtcgttcct gtgtctgagc
    16861 agttcgccat tcccgcgagc gccttcctgt tcgcaggagt ggcgtgcacg ggagtgtggc
    16921 tcgtcggttc gcgccgcggc gcgacgccgg aaatgctagt actcggcggc atcgcattcc
    16981 tgttcctgtt ccaggcactg ctgtcgctgc tccagttcat cgcctcgcag gaggccttgc
    17041 agcagatcgt gttctggttg tttggctcgc tgcagcgcgc gacgtggggc aaattttcga
    17101 tcgtggcgac ggttctaatt gtatcaatcc cggttctggc cgcagatgct tggcgtctga
    17161 ctgcgctgaa gctcggcgac gaccgcgcac ggggccttgg cgtcgacgtg accagcctgc
    17221 gcaagcggtg tttcgtgctg gtctcgcttc tgaccggagt cgctgtcgca ttcgtcggca
    17281 cgatcgggtt cgtggggctc gtcggcccac atgttgctcg catgctcgtc ggtgaggatc
    17341 agcgcacgtt cttgccggca tcggctctgt tcggcgctct tctgctttca ttggcttcga
    17401 ttgcttcgaa gacgatcgtt ccaggggcga tctttccgat cggcatcgtc acggcgctga
    17461 tcggcgtgcc gttcttcatc tggctcgtca tttcgaataa gagggcctac tggtgattgt
    17521 cgaagcgaga ggactaagcg tccactacgg cgacgtaacg gcagtcgatg gtatcgatct
    17581 aaccgtgacg cccgggacgc ttttggcgct ggtcggcccg aacgggtccg gcaaatcgtc
    17641 gctcgtcaag gcgatcgccg gcgtggtgcg agcggaaggc agcctgcagt tcggcggtcg
    17701 cccgcatcga cccggtacag tcgcgttcat ggcacaggac atcggcgggc aggctgcctt
    17761 gtcggtgctg gaagtcgtcc tgctcggtcg cttgcgcagg ctgggattga gcatcagcag
    17821 cggcgacact gctgcggtcg gagccgtcat ggacgaactc gacctcacac atctcgccgg
    17881 tcgccatcta ggcgaactca gcggcggtca gcgccagatg gtgttcctgg cacaagctct
    17941 cgcctccgac ccgcaggtgt tgctgctcga cgagccgatc agtgcgctcg atctgcgcca
    18001 tcagctggac gtgcttgatc tggtgcaacg gcacactaag acgcgaggcc tcgcgacggt
    18061 ttgcgttctg cacgatctga ccgcggctgc ccggatcgct gatcgcgtcg cgatgctccg
    18121 tcgcggccgg ctcgtggctg aagggccgtc cgctagaatc ctgacagccg accttcttgc
    18181 agacgtgttc gaggtcgagg cggaaatcct gacaacgcgg gcggggctcc ctgtcgtaag
    18241 cccgacccgg acccgatcga ggagcgatcc gtcgtcgcaa taggattgga agcagacagt
    18301 cgtcgtgcgc agatgtcgcg ccctcagcac ttctgctaag gccacgaccg ctgcttcgag
    18361 ttgggtactc gaccggccgt ggacatccac tagtcgcggc gtgtgccagg ggctatcgtc
    18421 gcctcggctc catcacgggg ggcgtgcacc tcaacagtca ttgctgcggg aggccgacat
    18481 ggccagaact gtcgccgaag agctcggcgc ctatgcggcg gccgagcttg cccgtccgct
    18541 cgcgcccgag atcgagcatc atgcgcggcg cgcgctcatc gactggtttg ctgcactctt
    18601 tccgggcacg ctcatctcgc cggcgcgcga gctgttcacg gccctcgacg acgagatcgg
    18661 ccacggcggc gcctgggtct acggtctcga ccggcgcgcg ccgatccgca cggccgccct
    18721 cgttaacggc gccgtctcgc atgctgtcga gttcgacgac atct
//
LOCUS       DAORNH010000050        18583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2291_length_18583_cov_2.706930, whole genome shotgun sequence.
ACCESSION   DAORNH010000050 DAORNH010000000
VERSION     DAORNH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18583
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_18583_cov_2.706930"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK264_07270"
     CDS             <1..205
                     /locus_tag="PK264_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD75720.1"
                     /translation="QKRYGARILVRGGRTEIVEGEGCARNIVLEFESFEAARAYAHSP
                     EYAAARKLREGAGILDMVLVEGP"
     assembly_gap    284..366
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<367..1313)
                     /locus_tag="PK264_07275"
     CDS             complement(<367..1313)
                     /locus_tag="PK264_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRD75721.1"
                     /translation="MDRTAGGRGREWLARVALVLLALGFALLLAELTLRISGLRYDRM
                     PFVSDPVLHHALPRDYHYISEDPDGVLPAFAITYDDLGRPADPDVGRRARAGAPCRIA
                     LIGDSFTVANEVPYRSSFAGLLDAASPCEVRNFAVGSYSPMLHGLLWRERIAAFKPDL
                     VIIQIFSNDIWDDQVYAKSARRDGAGRVTAVPGASGEGFAEWRRRLYVARLLRRATSA
                     VQWWWQNRGRAQTSIDGYVEENPELTELSLAEMRRLVADIRAANVPLLLTVVPSKVRL
                     LTGTMPGPAKTEFAERWQAWAEREGVRFVDLVAPFHAAKA"
     gene            complement(1435..1599)
                     /locus_tag="PK264_07280"
     CDS             complement(1435..1599)
                     /locus_tag="PK264_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75722.1"
                     /translation="MKPEDVTDPHGTWRRIKLWSPEIAVAVLLTIVAIAFVAGALWQV
                     DHQPLASAQH"
     gene            complement(1727..2569)
                     /locus_tag="PK264_07285"
     CDS             complement(1727..2569)
                     /locus_tag="PK264_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75723.1"
                     /translation="MTDIDFEAEYNNRQRVPEHTEIQARWAQASARFRNEAKADLDLT
                     YGPRERNRLDLYHSASGLAGSPLVLYVHGGYWQRGSREDTAFVARELVARGLSVAVAS
                     YTLCPDVSVIEIVGEMQAAVRMLWGRLRRRPVVVGHSAGGHLAASMLATSWAETGGLP
                     ADLVRSSYAISGVFDLPPLIGTSLNAALSLTPESAREASPLTWPPPSASRTSIAAVGG
                     QESQEFIRQSLDLTAAWSAAGVKAECVIVPDTNHFTIVDELCRAESAMVARIVELALA
                     SAKD"
     gene            2833..3618
                     /locus_tag="PK264_07290"
     CDS             2833..3618
                     /locus_tag="PK264_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75724.1"
                     /translation="MQLGKDIAAVVTGGASGLGAATARALAGKGARVALLDLNAAVGE
                     KVAKEIGGVFASCDVTNEASVEAALAAARKAHGQERVLVNCAGIAIGKRITRKNKDTG
                     AIEPHDLASFAKVIQVNLVGTFLMMARSAAGMQTLAPVGPDGERGVMICTASVAAEDG
                     QIGQVAYAASKGGVKAMTLPIARDLAQSGIRVVSILPGLFHTPMFDGLPDDARRALAA
                     SVPFPSRLGSPTEYAALACHICENTMLNGESIRLDGAVRLAPR"
     gene            3709..4608
                     /locus_tag="PK264_07295"
     CDS             3709..4608
                     /locus_tag="PK264_07295"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRD75725.1"
                     /translation="MSAATYVVLLTVAVTASAQGAAYIIDWTTSAIEDVIGEAPLPKP
                     PIITAAVSPARTVHDAAAAQPAAIEPARQPVRAAGGRGDPASRFYSGDGDTYRTVCVR
                     LCDGYFWTVSFSTTDDRFEQDSATCARSCGQQVRLFTYKVPGGRPEDMEDLSGRPYAK
                     LKTAFLFRSRFDASCRCKPDPWSAEASMQHRLYALQDAARKGDATARREHAELKTKLE
                     ALTPAVAVASVDPADAPAKPVPKTRTAATSSPTRAERAAASAPPGRMSLGGPASHEPA
                     RPARHSGPASRFRDWRVRAFEGQ"
     gene            4670..>4845
                     /locus_tag="PK264_07300"
     CDS             4670..>4845
                     /locus_tag="PK264_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75726.1"
                     /translation="MSSLLSLEGRNALVTGASSGLGAHFARTLARAGAAVAIAARRVD
                     RLEALAREISAGGG"
     assembly_gap    4846..4855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4856..5440
                     /locus_tag="PK264_07305"
     CDS             <4856..5440
                     /locus_tag="PK264_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75727.1"
                     /translation="AVQLDVTDPASVKAAFDTVAAELGGIDIVVNNAGIAAPAYTLKM
                     TEEEWRGVLSVNLDGVFRMAQEGARRMKAAGRGGSIVNIASILGLGVIKTLAHYSASK
                     AGVIQLTKAMALELARDRIRVNAIAPGYIETEMNSDFFTGEAGRRLIARVPAERLGKL
                     QELDGPLLLLASEAGSFMTGSVVTVDGGFLLAMA"
     gene            5460..6524
                     /locus_tag="PK264_07310"
     CDS             5460..6524
                     /locus_tag="PK264_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HRD75728.1"
                     /translation="MSDAVKGRARTLDQAKLPALAAWLCPTIAAEHVAITDASLLGGG
                     AVQENWRLDVEVTGGPRAGRHHWVLRTDAAARLSMSLGRPEEFRCIAAAFRAGVKVAE
                     PVALGEDAGIIGRPFMIQTLATGSAQGRRIVRDPQIGEMGPRLARELGAELARLHKIR
                     PPVTDLGFLDEPRRSASHVAVMALREAMDAGSGLRPALEFIFQWLIDEAPEPRPTALV
                     HGDFRTGNYMVDGGRLTAILDWEFCHWGDPREDLGWFSARCWRFGVDDKTAGGIASKA
                     DLLAGYNSVAAEPIAEAEIAYWEVLALARWGAIAALQGDRFLKDGERSLELALTGLMP
                     AEMELDALLAIEVIAKGRKT"
     gene            6521..6877
                     /locus_tag="PK264_07315"
     CDS             6521..6877
                     /locus_tag="PK264_07315"
                     /inference="COORDINATES: protein motif:HMM:NF040046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HRD75729.1"
                     /translation="MTTKRIAPEDLIALASEMLRDEIVPVLPAGQRYAAAMIANALEI
                     ARRGITGDTESAAWALLDQIYDEGEGTLDQLARDIRSGEIDTDTNPDLAARLKAIVQA
                     ELRVTNPRFLASRGVR"
     gene            complement(6870..7751)
                     /locus_tag="PK264_07320"
     CDS             complement(6870..7751)
                     /locus_tag="PK264_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD75730.1"
                     /translation="MRSWLFVPADSDRKLAKAEASGADVLILDLEDSVAPSRRPVARG
                     MVADYLKAARAKGSGPRVWVRVNPLDGSDWEADLAAVIGARPDGVIQPKPRSGDDVHK
                     LSIALGHAEKAAGLEGGATRILPIVTETSASVLHLNSYVGASSRMIAMSWGAEDLSAV
                     IGSSANREADGRFTSLYRLVRDMTLVAASAASVDAIDTVYVNFRDGAGLLAEAREALR
                     DGFTGKMAIHPDQVAPINQAFTPTVAEIAHARAIADVFAKSPDAGVASLDGKMLDRPH
                     LKLAERVLARAAAAGVT"
     gene            complement(7791..8423)
                     /locus_tag="PK264_07325"
     CDS             complement(7791..8423)
                     /locus_tag="PK264_07325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRD75731.1"
                     /translation="MLLLIDNYDSFTFNLVHYLGEIGATCEVVRNDKITVAAAIAMKP
                     KGIVLSPGPCTPNEAGICLDLIAKAGASIPILGVCLGHQAIGQAYGGKVIRAPLPMHG
                     KMSTVSTTGRGLFAGVPTRFEVTRYHSLVVERATLPPELRITAETADGLIMGLEHVSH
                     PVHGVQFHPESIASEHGHDLLANFLKVAGIAPKRRNSPERHIPAGLAVAR"
     assembly_gap    8619..8628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8736..>9435
                     /locus_tag="PK264_07330"
     CDS             8736..>9435
                     /locus_tag="PK264_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD75732.1"
                     /translation="MAGIASPPDTEDALLAALNQKNLAALCLSGGGIRSASFALGLLQ
                     ALAGLPRGANSAPAPEEQSLLFRFNYLSTVSGGGYIGAWLTAMLHRNGAPATHEALTR
                     ASPETHPEADRSRFLKGLPVEWVRAYANFITPKRGLFSVDTWHGFAIILRNMLLTWLI
                     ALPSFVMAVIGVIVAAVVIALARSIAVPVHWPALDFEPTHMVSIALAAVGLSLHVWGA
                     CYVLSAISDIETSGR"
     assembly_gap    9436..9445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9446..12040
                     /locus_tag="PK264_07335"
     CDS             <9446..12040
                     /locus_tag="PK264_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75733.1"
                     /translation="DKSRRPRHGVVMRAIMPILAAYYLYTLAVAIALPGWRPWEPLFD
                     RRAIGAISELVPVFGLPQGAVGASIRVLLDLLIFTPPLVAAAEPPSFFYGRLADWIAS
                     VLPVVAFFQRLVGAEVRLLLDPLVFSPPTLHGVAWKPSWAQSLGVVPWAVVIGPFGVL
                     ITSLATRALCRRPLTCGTMLLRLAFYGIPFSLMLLAGILLVMPHVGQAIAMCGVKGMP
                     VAVISDVGALTVLGLPWLVLSHLVADAFFSAAKATRHRRLSRTDASLTQRHELVREWT
                     GRFNGYLLLAGAAWLAVATTVLVLPSVLDDCLTSRGGKLLASVGGVSGAMTMLLGWSR
                     FSGDKRDGSGGSKITANVILAIVAPLFCIILAMGTANAVTIAATALWRSGAARETRGL
                     ETLFTDELRTAPLDEIWFAVQILLGMIAAVGVLSLITRQIDINRFSLHSFYRNRLQRA
                     FLGPSQRYEVMQELPAGTRRAPDEDLFTDFNVKADIRVAELHPAIAVGQMSLSAHRPA
                     WPLFPIINMALNTVSSSRYLWQERRAAPFTASPLHAGTSIVRNYGLKNELGAYRSSCS
                     YGGPGGLSLASAMAISGAAASPNMGYHSSPGITLLLALFNVRLGWWLGNPLNATTHRE
                     PGPRSALKPFITEALGQTNEWADYVYLSDGGHFENLGLYEMVLRRCRFIIVSDAGADG
                     QRHFADLGNAVRKVYIDHGIRITFRGLPALRDAIGAEEERIEPFRFAIGRIHYKEAHS
                     GVEGIEDGFILYVKPTLDINSLPVSVQSYALANPSFPHESTADQWFGESQFEAYRALG
                     AHIMGAILDEADRVAPGATGTIAEVLTALEKRGNPRAVTAMTSRLAGDAAWPPKSKLV
                     RLWRKTRH"
     gene            11989..12483
                     /locus_tag="PK264_07340"
     CDS             11989..12483
                     /locus_tag="PK264_07340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD75734.1"
                     /translation="MAPKIEARPLVAEDEALIRAWIAEPEVRAWWGDAASAEAAIAIA
                     LTSATAVTRIMTADGRPFGYGHAVDDGIPGHAGRRSGLGAWRIDAFIGDAAFRGRGYG
                     PAILDAMALEVFATTLALAVSVIVPLRHERVARAAERAGFTWSEIVQEPGLGRCWLML
                     RDRP"
     gene            complement(12570..12746)
                     /locus_tag="PK264_07345"
     CDS             complement(12570..12746)
                     /locus_tag="PK264_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRD75735.1"
                     /translation="MQMLARFVKCESGATAIEYGLIAALIGVAIITVVNNAGTEIKGT
                     FTDVETGLKKRPAV"
     gene            complement(13032..14378)
                     /locus_tag="PK264_07350"
     CDS             complement(13032..14378)
                     /locus_tag="PK264_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRD75736.1"
                     /translation="MRNDGAELEAGAPLASWERLLGPAVAVATAAALMAPRATPGLLL
                     IVAGAILIAARQAAPDKVSLRPPTGYVIAVGGVLGLYLLVNASWSPARGEAYGKVLFY
                     WVAFAAVSVAAAAATSAWSGRGVGDERYRRLAMGLVAGLAVGSLFLAIESAFAQPIKR
                     AIYSVLAFARPDPKHVKVAADGAVSEIGHYVLNRGIAVMCLLLWPLLAIIARLWPGGA
                     LRIALPAGLVALAALAVARSEHETSMLALLAACVTYVGMRVSAKTMQWLVHGGWLLAT
                     VLMVPAALHAHKAGLHSASWIPQTGRNRIVLWNVTAQEAMKRPVFGVGVAATKELDEA
                     RIPVAKVEAGNTYPERTGRHAHNIYLQAWYELGGVGALLLLAVGAAMLAAMARMPSAT
                     QPFAFAGFVSAAVIGGFSWGIWQVWFMAAFALAALLQVFAASAWPLSDAKSSHDDE"
     gene            14584..15810
                     /locus_tag="PK264_07355"
     CDS             14584..15810
                     /locus_tag="PK264_07355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD75737.1"
                     /translation="MSTAQSSARTPILPRADAASLGFSTERLGRIRSSMEREVSAGLL
                     PGGVLAVARRGRLAYLEAFGQRDAATGAPMWADAVFSIASMTKPMTSAAILMLHEEGR
                     LLLGDPVSKHLPALAGMRVAKADGSGETVPADREMTVQDLLRHTSGLTYRDRGTTPAH
                     KLYPGSSIVATVRHTREEFLALLQKAPLIYQPGTVWEYGFSTDILGLVVEAVSGQSLG
                     QFLAERLWRPLGMVDTSFTLSDAQRSRYARAFPKCPITGDPLTVHHATAEIPKWESGG
                     GGAVSTAADYIRFAQMLLDGGTLGGQRILGRKTVELMTADHLTPSIVNRITTMDAACD
                     GYGFGLGFAVRRGAGGAALMGSAGDYYWSGVYGTYFWIDPAEQLAAVFMAAAPGMIRL
                     RYRQLMRGLVLQAIEA"
     gene            15818..16276
                     /locus_tag="PK264_07360"
     CDS             15818..16276
                     /locus_tag="PK264_07360"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HRD75738.1"
                     /translation="MTSVRWPDLAGRLDADARGRVHVLPVRVYFEDTDFSGVVYHASY
                     IRWCERGRTDFLRHSGNDHRTLIDGGDGREPAAFMVRRMQIEFLRPARIDEILEVTTR
                     IGEIGAATLELHQAVRRGETTLFEAKVLVVLVSTAGKPLRIGPRLRAALA"
     gene            16273..16998
                     /locus_tag="PK264_07365"
     CDS             16273..16998
                     /locus_tag="PK264_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRD75739.1"
                     /translation="MNDGVTKMSPEHTTDAARELWVFGYGSLMWRPGFPHVETHRARL
                     AGFSRCFCVYSVHHRGRKGRPGIVLGLDRGGTCDGMVFRVPDEHAASTLAYLRERELV
                     YGVYRETWHEVSLLDDGGRIVRAVTYIAERAHPSFAGRQPVGKLARLIRGAEGVSGPN
                     IEYLVNTVRQLTLMQADAGELRRILSLISPYAATAGDGAATRARCLAMMRHHNHTRRR
                     RPPGPRITLERLNRFLFRMRIAS"
     gene            complement(17012..18403)
                     /locus_tag="PK264_07370"
     CDS             complement(17012..18403)
                     /locus_tag="PK264_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRD75740.1"
                     /translation="MTADGRLTFRQWLLLAVIVGIGALIALQAPPSASPKAMLGLGIV
                     AATVLLWASAVIPAWASGLLFFAAGLASAIVPPLKLLSGFWSTAAMLILGGLILGAAA
                     ERSGLGRWIARGLVERFVTSYPRFLLGILLGSGVLSFLVPATMGRLALTIPIVGAIAK
                     AAGYVAGSSGYNGAMLVTVAGNFLTSYAVLTGNLVNVIAIGTIEARHGPIIQYGEYLL
                     YTLPVLGIVKGATFLAIVVAFFSAPPPALPKPEDAPVLTPAGRRLSLLLAVTLLLWAT
                     DFWHRLPPGVVALAAGIVCLLPYPGLVTWRESFDPNKATAVLSLAAVLGVATVLTHSG
                     ASDLIGRELARLAAVEGRSAIYGFSAIAVMSALTATVATIVGAVATVSPVIDVIAAAT
                     GLSLKMGLIAELTGLQAVFFPFEAVPLMVGLAIARVPARDALRALIPAAAVGLVVILP
                     LQIGWLGLIGALR"
     gene            complement(18400..>18583)
                     /locus_tag="PK264_07375"
     CDS             complement(18400..>18583)
                     /locus_tag="PK264_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRD75741.1"
                     /translation="DGARGKAAADRDAVARAIVALSDLAVAHPEIEELDVNPVFAGSD
                     GVAAVDWLMRRRGDPA"
BASE COUNT         3118 a         6269 c         6157 g         2926 t
ORIGIN      
        1 gcagaagcgc tacggcgcgc gcatactcgt ccgcggtgga cgcaccgaga tcgtcgaagg
       61 tgaaggctgt gcacgcaaca tcgtcctcga gtttgagagt ttcgaagcgg cgcgcgccta
      121 cgcccattcg cccgagtatg ctgccgcccg caagctccgc gaaggcgcag gtatcctaga
      181 catggtgctc gtcgaaggac cgtgagggtc gctgaagcaa ggcagggcgg ccgcgagcct
      241 atttcagttc gctgcaatcg cccttccgcg agagtcgccg cggnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnngcct tggccgcatg aaacggcgcc acgagatcga cgaagcggac gccctcacgc
      421 tcggcccagg cctgccagcg ctcggcaaac tcagtcttgg ccggtcccgg catcgtgccg
      481 gtgaggagac gcaccttgga gggcaccacg gtcagcagga gcggcacgtt cgcggcccgg
      541 atgtcggcga cgaggcggcg catctcggca aggctcagct cggtcagttc ggggttctcc
      601 tcgacataac cgtcgatgga ggtctgcgca cgcccacggt tctgccacca ccactgcacc
      661 gcgctcgtgg cccgccttag gaggcgcgcc acataaagcc gccgccgcca ctcggcgaag
      721 ccctccccgc ttgcgccagg aaccgccgtg acgcggcccg cgccatcacg gcgcgcgctc
      781 ttcgcgtaga cctggtcgtc ccagatgtcg ttcgagaaga tctggatgat cacgaggtcc
      841 ggcttgaagg cggcaattcg ctcgcgccag aggaggccgt gcaacatggg gctgtagctg
      901 ccgaccgcga agtttcggac ctcgcacggt gatgcggcat cgaggagccc ggcaaaggac
      961 gagcggtagg gcacctcgtt agcaacggtg aagctgtcgc caatgagcgc gatgcggcat
     1021 ggagccccgg cgcgagcgcg gcggccgaca tctgggtccg cgggccggcc gagatcgtca
     1081 taggtgatgg caaacgcggg gaggacaccg tcggggtcct cggagatgta atggtagtca
     1141 cgcggcagcg cgtgatgcag gaccgggtcg gagacgaacg gcatgcggtc gtatcggagg
     1201 cctgagatgc gcagcgtcag ctcggcgagc agcagggcaa agccgagggc caacaggacg
     1261 agcgccaccc gtgcgagcca ctcccgcccg cggcctccgg cagtgcgatc catgcgcgct
     1321 cactccagcc cgttttcgca atggcactgc cccgccgaca aggaacacgc gcgacctccg
     1381 tgccgcaaga ccgggctgcc acatcggtaa actcagggac cttgccgccg tgcttcagtg
     1441 ctgcgcggag gcgagcggct gatgatcgac ctgccagagt gcaccggcca cgaaggcgat
     1501 ggccacgatg gtcaggagga cagccacggc aatttccggc gaccacagct tgatgcggcg
     1561 ccacgtaccg tgcggatctg taacgtcttc cggcttcatc ccgccaatct gcctcagagg
     1621 tgagtgcgtc ggccaggatg ccaaaggccg cgcgacagat cggcacattc cgcgctgccg
     1681 acacacgagc ggcgcatcgg cgccatcttc ctctccccca agcgcatcag tcctttgccg
     1741 aggcgagggc aagctccacg atgcgggcca ccatggcgct ctcggcacgg caaagctcgt
     1801 ccacgatggt gaagtggtta gtgtcgggaa cgatcacgca ctcggctttc acgcccgcag
     1861 ccgaccaggc cgccgtcaga tcgaggctct ggcggatgaa ctcctgactc tcctgaccac
     1921 cgacggcggc gatcgacgtg cggctcgccg agggtggcgg ccaagtcaga gggctcgcct
     1981 cgcgggcgct ctcaggggtg agggacaagg ccgcgttgag ggacgttccg atgagcggcg
     2041 gcagatcgaa cacgccgctg atcgcatagc tcgagcgcac gagatcggcg ggcaggccgc
     2101 ccgtctcggc ccagcttgtc gccagcatcg atgcagcgag atgccccccc gccgaatgcc
     2161 cgacgacaac cggccgccgc ctgagtcgtc cccagagcat gcgcaccgcc gcctgcatct
     2221 ctcccacgat ctcgatcacc gatacatcag gacacagcgt ataggatgcg acggcgaccg
     2281 agaggccacg ggcaaccagc tcacgcgcca cgaaggccgt atcctcgcgt gacccgcgct
     2341 gccagtagcc gccgtgcacg tagaggacga ggggggagcc cgcgagaccc gaggctgaat
     2401 gatagagatc gagccggttt cgctcgcgcg ggccgtaggt gaggtcgaga tcagctttgg
     2461 cctcgttgcg gaaccgcgcc gacgcctgcg cccagcgcgc ctggatttcg gtatgctccg
     2521 gcacgcgctg gcgattgttg tactcggctt cgaaatcgat gtccgtcatg ccggccccgt
     2581 ccccatcgcc gccccggaac aagagttttc cagaaaaata ggacactgtt caagcgccaa
     2641 tcgcggcgcg tgacctgcga ccaatggagc atcgcggcgt tcggtcgccg gcaggcggac
     2701 gacgcgaacg agccccgcgg gacgccaatt gaccttgaat atcagacgac gccctacaac
     2761 cgcttgctgc accgcgacaa tcggcctatg aggtccattt caggcaacgt aagaattgtg
     2821 caggagggcg ccatgcagct tggcaaggac atcgcagccg tcgtcaccgg tggtgcgtcc
     2881 ggtctcgggg ctgcgacagc gcgggcactg gcggggaagg gcgcgcgcgt cgcgctgctc
     2941 gatctcaacg ccgcggtggg cgagaaggtc gccaaggaaa tcggcggagt gttcgcgagc
     3001 tgcgacgtca ccaacgaggc gagcgtcgag gcggcgctgg cagcggcgcg caaggcgcat
     3061 gggcaggagc gcgtcctcgt caattgcgcc ggcatcgcca tcggcaagcg gatcacgaga
     3121 aagaacaagg atacgggtgc gatcgagccg cacgacctcg cgagcttcgc caaggtcatc
     3181 caggtgaacc tcgtcggcac cttcctgatg atggcgcgct cggcagccgg catgcagacc
     3241 cttgcgccag ttggtcccga cggcgaacga ggcgtgatga tctgcacggc ctccgtcgcc
     3301 gcagaggatg gccagatcgg tcaggtcgct tacgctgcct ccaagggcgg cgtaaaggcg
     3361 atgacgctgc cgatcgcgcg cgatctcgcc cagtccggca tccgcgtggt gtcgatcctg
     3421 cccgggctct tccacacgcc gatgttcgac gggctcccgg acgacgcccg ccgggcgctg
     3481 gcggcgtcgg tgccgttccc ctcgcgcctc ggctcgccca ccgagtatgc agcactcgcc
     3541 tgccacatct gcgaaaatac aatgctcaac ggcgaatcga tccgtctcga cggtgctgtc
     3601 cgccttgcac cgcgctgacg accccgcgtc ccctgcccga gtatcgaagt acagtactgc
     3661 gtcatgcacc tcgttcgttg gactgccggt catcgccgaa ccacgacttt gtcggccgcc
     3721 acctacgtgg tgttgctgac cgtcgccgtc acagcgtccg cccagggggc tgcctacatc
     3781 atcgactgga cgacgagcgc catcgaggat gtgatcggcg aggccccctt gccgaagccg
     3841 ccgatcatca ccgcggcggt gagccctgcc cggactgtcc acgatgcggc agcggctcag
     3901 ccggcagcga tcgagcctgc tcgacagccg gttcgagcag cgggcgggcg cggcgatccc
     3961 gcctcgcgct tctattcggg cgacggcgac acctaccgca cggtctgcgt ccgcctctgt
     4021 gacggctact tctggacggt cagcttctcg accacggacg atcgtttcga gcaggacagc
     4081 gcgacttgcg cgcggagctg cggacagcag gtccgcctct tcacctacaa agtcccgggt
     4141 gggcggcccg aggacatgga ggatctttct gggcggccct acgcgaaact gaagaccgca
     4201 ttcctattcc gctcgcgctt cgatgcgagc tgccgctgca agcccgatcc ctggtcggca
     4261 gaggccagca tgcagcatcg gctctatgcc ttgcaggatg cagcccgcaa gggcgacgcg
     4321 acggcccgcc gcgagcacgc cgagctcaag acgaagctcg aggccctcac acccgccgta
     4381 gccgtggcat cggtcgatcc tgccgatgcg ccggcaaagc ccgtaccgaa gaccaggacg
     4441 gctgccacct catccccgac gagagccgag cgggctgcgg cctcggcccc tccgggacgc
     4501 atgagcctcg gtggaccggc gtcgcacgaa ccggcgagac ccgcgcgcca ttcgggcccg
     4561 gccagccgct tccgcgactg gcgcgtcagg gcgttcgaag gccagtgaca ggacggcgct
     4621 tccgaggcat atcggcggga cgcttctgcc ggagacctgc cccgtgtcga tcagctcgct
     4681 tctttcactc gagggccgca acgcacttgt caccggtgcc tcgtcggggc tcggcgcgca
     4741 ttttgccagg acgctcgcgc gcgcaggcgc tgcagttgcc atcgctgcac gacgcgtcga
     4801 ccgtctcgag gcgctcgcgc gggagatctc ggcaggcggc ggcaannnnn nnnnngccgt
     4861 ccagctcgat gtgaccgacc ccgcgtcagt caaagcagcc tttgacactg tggcggccga
     4921 gctgggcggc atcgacatcg tcgtcaacaa tgcgggcatc gcggcgccgg cctatacgtt
     4981 gaaaatgaca gaggaggagt ggcgcggcgt gctctccgtc aacctcgacg gcgtcttccg
     5041 catggcgcag gagggcgcgc ggcgcatgaa ggccgcgggt cgcggcggat cgatcgtcaa
     5101 catcgcctcg atcctggggc tcggcgtgat caagacgctc gcccactact cggcctcgaa
     5161 ggcgggcgtg atccaattga cgaaggccat ggcgctcgag cttgcgcgcg accggatccg
     5221 cgtaaacgcg atcgcgcccg gctacatcga gacggagatg aacagcgact ttttcacagg
     5281 cgaggccggc cgccgcctca ttgcccgggt gccggcggag cggctcggaa aactccaaga
     5341 gctcgatggc ccgctcctgc tcctcgcgtc ggaggctggc tcgttcatga ccggcagcgt
     5401 cgtcacggtt gacggcggat tcctgttggc gatggcttga gcttggaggc ggcgaggcaa
     5461 tgtcagacgc agtgaagggc cgcgcgcgga cgctcgatca ggcgaagctg ccagcgctcg
     5521 cggcatggct ctgcccgacg attgccgccg agcacgtggc gatcacggac gcctcgcttc
     5581 tcggcggcgg cgctgtgcag gagaactggc ggctcgacgt cgaggtgacg ggagggccgc
     5641 gcgcggggcg gcaccactgg gtgctgcgga cggatgctgc tgcccgtctc agcatgagcc
     5701 tcggccggcc cgaggaattc cggtgcatcg cggcggcatt ccgtgccggc gtgaaggtgg
     5761 ccgaaccggt cgcactcggc gaggatgccg gcatcatcgg ccgccccttc atgatccaga
     5821 cgctggccac ggggagtgcg cagggccggc ggatcgtccg tgatccacag atcggcgaaa
     5881 tgggaccccg ccttgcgcgc gaactcggcg ccgagctcgc ccgtctccac aagatccgcc
     5941 cgccggtgac cgatctcggc tttcttgatg agccgaggcg atccgcctcg catgtcgccg
     6001 tgatggcgct gcgggaggcc atggatgcgg gctccggctt gcgcccggcg ctcgaattca
     6061 tcttccagtg gctcatcgat gaggcgccgg agccgcgccc aacggccctg gtgcacggcg
     6121 atttccgcac cggcaactac atggtggatg gtggtcggct caccgccatc ctcgactggg
     6181 aattctgcca ctggggcgac ccgcgcgagg acctcggctg gttctcggcg cgctgctggc
     6241 gctttggcgt cgacgacaag accgctggcg gcatcgcgag caaggccgat ctgctcgcgg
     6301 gctacaacag cgtcgcggcc gagccgatcg ccgaggccga gatcgcctac tgggaagtac
     6361 tggcgctcgc ccgctggggt gccatcgccg cgctgcaggg cgatcgcttc ctgaaggacg
     6421 gcgagcgctc gctcgagctt gcgctgaccg gcctgatgcc ggcggaaatg gagcttgacg
     6481 cgctgctcgc catcgaggtg atcgccaagg gacgcaagac atgacgacga aacggatcgc
     6541 gcccgaggac ctgatcgcac tcgcgagcga gatgctccgc gacgagatcg tcccggtcct
     6601 cccggcgggc cagcgctacg cggcggccat gatcgccaac gcgctcgaga tcgcccgccg
     6661 cggcatcacg ggcgacacgg agagtgcggc ctgggcgctc ctcgatcaga tctatgacga
     6721 gggcgaaggc acgctcgacc agctggcgcg cgacatccgc tcgggcgaga tcgacaccga
     6781 caccaatccc gatctcgcgg cacgactgaa ggcgatcgtt caagccgagc tccgcgtcac
     6841 caacccgcgc ttcctagcaa gccgcggcgt caggtgacac cggccgccgc ggcgcgcgcg
     6901 agcacgcgtt cggcgagctt cagatgcggt cgatcgagca tcttgccgtc gaggctcgcg
     6961 acacccgcgt cggggctctt ggcgaacacg tcggcaatgg cgcgcgcgtg cgcgatctcg
     7021 gccactgtgg gcgtgaaggc ctgattgatc ggcgccacct gatcgggatg aatcgccatc
     7081 ttgcccgtga aaccatcgcg cagcgcctcg cgcgcctcgg cgagcagccc ggcgccgtcg
     7141 cggaagttca cgtagaccgt atcgatcgca tcgacgctcg ccgccgaagc ggcgaccagc
     7201 gtcatgtcgc gcacaaggcg atagagcgag gtaaagcggc cgtccgcctc gcgattggct
     7261 gacgagccga tcacggccga gaggtcctcc gcgccccagc tcatcgcgat catgcgcgag
     7321 ctcgctccaa cataggagtt gaggtgcagc accgaggcgg acgtctccgt gacgatcggc
     7381 aggatgcgcg tcgccccacc ctcgaggccg gccgccttct cggcgtggcc gagggcgatc
     7441 gagagcttgt gcacgtcgtc acccgaacgc ggcttcggct ggatgacgcc atcggggcgc
     7501 gcaccgatca ccgccgcgag atccgcctcc caatcggacc cgtcgagcgg attgacgcgg
     7561 acccagaccc tcgggccgct gcctttcgcg cgcgcagcct tcaggtagtc ggcaaccatg
     7621 ccgcgggcga ccggacggcg ggagggtgcc accgaatcct cgaggtcgag gatcagcacg
     7681 tcggcgcccg aggcctcggc cttggcaagc ttgcggtcgc tgtcggccgg cacgaagagc
     7741 caggagcgca tgatcggtgt ccgtcgtgtt cgaggagtgt tagggcaggc ctaccgcgcc
     7801 acggcgaggc cggccggaat gtgccgctcg ggcgaattgc gccgcttcgg cgcgatgccc
     7861 gcgaccttca ggaagttcgc gaggaggtcg tggccgtgct cggaggcgat gctctcgggg
     7921 tggaactgca cgccgtgcac gggatgggaa acgtgctcaa gccccatgat gaggccgtcc
     7981 gccgtctcgg ctgtgatgcg cagctcgggc ggcaacgtgg cgcgctcgac gacgagcgag
     8041 tgatagcgcg tcacctcgaa gcgcgtcggc acacccgcga agagcccacg gcccgtcgtc
     8101 gagacggtcg acatcttgcc gtgcatgggc aagggcgcgc ggatgacctt gccgccgtag
     8161 gcctgcccga tcgcctgatg gccgagacag acgccgagga tcgggatcga ggcgcccgcc
     8221 ttggcgatga gatcgaggca gatgccggcc tcgttcggtg tgcagggtcc gggcgagagg
     8281 acaatgcctt tgggtttcat ggcgatcgcc gccgcgacgg tgatcttgtc gttgcgcacg
     8341 acttcgcaag tcgcgccgat ctcccccaaa tagtggacga gattgaaggt gaaactgtcg
     8401 taattgtcga tgaggagcag catctgcggt tccggcactt cgggtggtcc cgcccggctg
     8461 ccggagcgcg gggccacttg ctccccgctc cggcctcggt cgggagcgcg tcaaatcgag
     8521 actgaacgct tttttcgtag ccctgaattt ggtggatcgc aatgaccagc tcggccgata
     8581 cgcccgcgac tacaccgccg cgacaggccg gccgctgtnn nnnnnnnncg cccgccgccg
     8641 ctccgaccac tgcgaccgag gcagcgccgt cccgcccgag ggaggacggc gcatcgctga
     8701 gccgggtcca cgtgctcgcc gaagaagccc gccaagtggc ggggattgca agcccacccg
     8761 ataccgagga cgcactgctt gccgcactga accagaagaa cctcgctgcc ctctgcctct
     8821 caggcggcgg catccgctcg gcatcgttcg ccctcggcct gctgcaggcg ctggccggac
     8881 tgccgcgtgg ggccaactcg gcgcccgcac ccgaggagca gagcctgctc ttccggttca
     8941 actacctctc gacggtctcc ggcggcggct acatcggcgc ctggctcaca gccatgctgc
     9001 atcggaacgg cgcaccggca acccatgagg cgctcacccg agcgagcccc gagacgcacc
     9061 cggaggcgga tcgcagccgc ttcctcaaag gcctgcccgt cgagtgggtg cgggcctacg
     9121 ccaacttcat cacgccgaag cggggcctct tctccgtcga tacctggcat ggcttcgcga
     9181 tcatcctgcg caacatgctg ctgacgtggc tgatcgcgct gccgagcttc gtgatggcgg
     9241 tgattggggt catcgtcgcc gctgtcgtca ttgccctggc gcgcagcata gcggtgccgg
     9301 tgcactggcc ggcgctcgat ttcgagccga cccacatggt ctcgatcgca ctcgcggcag
     9361 tcggcctgtc actccacgtc tggggggcgt gctacgtgct ctccgcgatc agcgacatcg
     9421 aaacaagtgg ccggtnnnnn nnnnngacaa gagccggcgg ccgcggcacg gcgtcgtgat
     9481 gcgcgccatc atgccgatcc tcgcggccta ctacctctac acactcgccg tcgcaatcgc
     9541 gttgcctggg tggagaccct gggagccgct gttcgatcgc agggcgatcg gagcgatcag
     9601 tgagctcgtc ccggtgttcg ggctgcctca gggtgccgtc ggggcgagca tccgcgtgct
     9661 cctcgacttg ctcattttca cccctccgct agtcgcagcg gccgagccac cgtccttctt
     9721 ctatgggaga ctcgcggatt ggattgcctc ggtcttgccg gtcgttgcct ttttccaacg
     9781 tctcgtcggc gccgaggtac gcctactgct cgacccactc gtcttctccc ctccaacgct
     9841 ccacggcgtc gcttggaagc catcctgggc gcaaagtctc ggcgtggtgc cgtgggcggt
     9901 ggtcatcggt cctttcgggg tgctgatcac ctcgctcgcc actcgggcgc tctgccgtcg
     9961 gcctctcaca tgcgggacga tgctgctgcg gctcgccttc tacggcatcc cgttcagcct
    10021 gatgttgctc gccggcatac tgctcgtgat gccccatgtc ggccaggcga tcgctatgtg
    10081 cggggtcaag ggcatgccgg tagcggtgat cagcgacgtg ggtgcgctaa ccgtgctcgg
    10141 cctgccttgg ctcgtcctct cgcacctcgt cgcggacgca tttttctccg cggcaaaagc
    10201 gacacgccac cggcggctct cgcgtacgga tgcctcactc acccaacgtc atgagctggt
    10261 tcgcgaatgg acgggacgct tcaatggcta cctactgttg gcaggcgccg cctggctcgc
    10321 ggtggccaca acggtccttg tgctgccctc ggtgctcgac gattgtctga cgagcagagg
    10381 cggcaagctg ctggcctctg tcggcggcgt ctcaggtgcc atgacgatgc tgctcggctg
    10441 gagccgcttc agcggcgaca agcgagatgg atcaggtggc agcaagatca ccgcgaacgt
    10501 cattctggcg atcgtcgccc cgctgttctg catcatcctg gcgatgggca cagccaatgc
    10561 cgtcacgatc gccgcgacgg ccctctggcg cagtggtgca gcccgcgaga caagggggct
    10621 cgagaccctc ttcaccgacg aactgcgcac ggcgccgctc gatgagatct ggtttgcggt
    10681 gcagatcctt ctcggaatga tcgcggcggt cggcgtcctt tcgctcatca cccggcagat
    10741 cgacataaac cgcttctcgc tccactcgtt ctatcgcaat cggctgcaac gcgcgttcct
    10801 cggaccgagc cagcgctatg aggttatgca agaactccct gctggtacgc ggcgtgcgcc
    10861 ggatgaggat ttgttcacgg acttcaacgt gaaggccgat atccgggtgg cggaacttca
    10921 tccggccatc gcagttggtc aaatgagcct ctcggcgcac aggcccgcct ggccgctctt
    10981 ccccatcatc aacatggcgc tcaacacggt gagttcgtca cgctacctct ggcaggagcg
    11041 gcgggcggca cccttcacgg cgagcccgtt gcacgcgggc acatcaatcg tgaggaacta
    11101 cggcctcaag aatgagctcg gcgcctatcg gagcagctgc agctacgggg ggcctggagg
    11161 gttgtcgctc gcctctgcca tggcaatctc cggggccgcg gcgagcccta acatgggcta
    11221 ccactcctcg cccggcatca cgctcctcct ggccctcttc aatgtgcggc tcggctggtg
    11281 gctcggcaat ccgctgaacg ccaccaccca tcgcgagccc gggcctcgct cggctttgaa
    11341 gccgttcatc accgaggcgc ttgggcaaac caacgagtgg gccgactacg tctacttgtc
    11401 cgacggcggg cacttcgaga acctcgggct ctacgagatg gtgctgcgcc gctgccggtt
    11461 catcatcgtc agcgacgccg gcgccgacgg tcagcgccac ttcgccgacc tcggcaatgc
    11521 ggtgaggaag gtctacatcg atcacggcat ccgcatcacc ttccgcgggc tcccggcgct
    11581 gcgcgacgcg atcggcgccg aggaggagcg catcgaaccg ttccgctttg cgatcgggcg
    11641 catccactac aaggaggcgc actccggcgt cgagggcatc gaggacggct tcatcctcta
    11701 tgtgaagccg acgctcgaca tcaactcgct ccccgtgagc gtgcagagct atgccctcgc
    11761 gaacccgtcg ttcccgcacg agtcgacggc cgaccaatgg ttcggcgaaa gtcagttcga
    11821 ggcctaccgg gcgctcggag cgcacatcat gggggccatc ctcgacgagg cggaccgcgt
    11881 cgcccccggt gcgaccggaa caatcgctga ggtgctgaca gcgctcgaga aacggggcaa
    11941 tcctcgcgcc gtgacggcaa tgacctccag gttggcggga gacgcggcgt ggcccccaaa
    12001 atcgaagctc gtccgcttgt ggcggaagac gaggcactga tccgcgcctg gatcgcggag
    12061 cccgaggtgc gcgcctggtg gggggatgcg gcaagcgccg aggcggcaat tgcaatcgcg
    12121 ctcacgagcg cgaccgcggt cacacgcatc atgacggcgg acggacggcc gttcggttat
    12181 ggccatgccg tcgatgacgg cattccgggc catgccggcc ggcgttccgg cctcggtgcc
    12241 tggcggatcg atgctttcat cggagacgct gcatttcggg ggcgaggcta tggacctgca
    12301 atcctcgacg cgatggcgct cgaggtcttc gcgacgaccc tggcgctcgc cgtctccgtg
    12361 atcgtgccgc ttcgccacga gcgtgtcgcg cgtgccgcgg aacgagccgg gttcacctgg
    12421 tccgagatcg tccaagagcc tggcctcggg cgctgctggc tgatgctgcg tgaccgcccc
    12481 tagtctgccc gagaaagcga aaccgcccgg ccggggggcc aggcggctcg cagtcccgag
    12541 gacgccgcgt agcgcgacgc cgagggatat tagacggccg ggcgcttctt gaggccggtc
    12601 tcgacgtcgg tgaacgtgcc cttgatctcg gtgccggcgt tgttcacgac cgtgatgatg
    12661 gcgacgccga tcagggccgc gatgaggccg tactcgatgg ccgttgcacc agactcgcac
    12721 ttcacgaagc gggcaagcat ctgcatttcg attactccaa ctttactgga cacaccttgc
    12781 gcaccctgcg ctctgctcga ccgacgcctc gtcgcgccga acatgtgatg aaggtagccc
    12841 catcctctta aggacccctg aatcgcgcag cagacgcagg agggtaaagg tcgacttaac
    12901 aaatacaacc atggcatttc taagcatgca catagtaata atagtaacat atttgttaaa
    12961 cccggtattt aaattcgcct cttggcggac tacaattgat tttctccagt tatatttggt
    13021 taataatttg cttattcgtc gtcgtgactt gatttcgcat cagagagtgg ccaagcgctc
    13081 gccgcgaaca cctgcagaag cgcagcaagt gcgaacgcgg ccatgaacca gacctgccag
    13141 ataccccacg agaagccgcc gatcacggcc gccgagacaa accctgcgaa tgcgaacggc
    13201 tgagtcgcgg acggcattcg cgccatcgca gcaagcatcg cggcaccaac cgccagcagc
    13261 agcaaggcac cgacgccgcc aagctcatac caggcctgga gatagatgtt gtgggcatgc
    13321 cgcccggtcc gctcgggata ggtattgccg gcctcgacct tggcaacagg gatgcgcgcc
    13381 tcgtcgagtt ccttcgtagc ggccaccccg acgccgaaca ctggccgttt catggcctcc
    13441 tgcgccgtga cattccagag cacgatgcgg ttgcggccgg tctgcgggat ccagctcgcg
    13501 ctgtgcaagc cggccttgtg cgcgtgaagg gcggccggga ccatcagcac cgttgccagg
    13561 agccatcccc catggaccag ccactgcatg gtctttgccg aaaccctcat gccgacatag
    13621 gtcacgcagg ctgcaagcag cgcgagcatc gatgtctcat gctcggagcg ggcgacggcg
    13681 agcgcggcaa gcgcgacaag gccggccggc agagcgatac gcaaggcgcc acccggccag
    13741 aggcgcgcaa taatcgcaag cagaggccaa agcagaaggc acatgaccgc gatgccgcga
    13801 ttgaggacgt agtgcccgat ctcgctcact gcgccgtcgg ccgcgacctt tacgtgcttg
    13861 ggatcgggcc gcgcaaatgc aagcacgcta tagatcgccc gcttgatcgg ctgcgcgaag
    13921 gcagattcaa tcgcgagaaa cagcgagccg actgcgagcc cggcaacgag acccatcgcc
    13981 aggcgccggt aacgctcgtc ccccacaccg cgtccagacc aagccgaagt cgcggcagcg
    14041 gctgccacgc tgacggcggc aaaggcgacc cagtagaaca gcaccttccc gtacgcctcg
    14101 ccgcgcgcag gagaccaact tgcattgaca agcaagtaca gaccgagcac accgccgacg
    14161 gcgatcacgt agccagttgg cggccgaagc gataccttgt cgggtgcggc ctggcgcgcg
    14221 gcaatcagaa tcgcgccagc cacgatcagc aacaaccctg gcgtggcgcg cggtgccatc
    14281 agagcagccg ccgtcgcgac ggccacggcc ggaccgagca accgttccca ggacgcgagt
    14341 ggtgcacccg cttcgagttc cgccccgtcg ttgcgcatgc gtgctccggg cgttgttgat
    14401 ccgcgccggg atctcggttc tgccgccaag cgtgctatcg gctaagcccc ggcgggtcaa
    14461 catcgtgagc tgccccgtcg accacgcacc tccatcgtgt ggcacgcaca ccagcgcgtg
    14521 gctcttgcct cgcgggcgtg gcactggcac tcaggaggcc tgatcccggt cggaggctct
    14581 cccatgtcca ctgcccaatc ctcagcgcgt acccccatcc tgccccgcgc ggatgccgcc
    14641 tcgctcggct tctcgaccga gcgcctcggc cgcattcgga gctccatgga gcgcgaggtg
    14701 agtgccggcc tcctgccggg cggcgtgctc gccgtcgcaa ggcgtggccg cctcgcctat
    14761 ctcgaggcct tcggacagcg cgacgcggca accggcgcgc cgatgtgggc ggatgccgtc
    14821 ttctcgatcg cctcgatgac gaagccgatg acctcagcag ctatactgat gctgcacgag
    14881 gaaggccgcc tgctgctcgg cgatcccgtc tcgaaacacc tgccggcgct tgcagggatg
    14941 cgcgttgcaa aggcggacgg ctcgggcgag acagtgccgg ccgaccgcga gatgacggtg
    15001 caggacctcc ttcgccacac ctccggcctc acctatcgcg atcgcggcac gacgccggcc
    15061 cacaagctct atccgggctc gtcgatcgtg gcgaccgtgc ggcacacgcg cgaggagttc
    15121 ctggcactcc tgcagaaggc gccgctcatc taccaaccgg ggacggtctg ggagtacggc
    15181 ttctcgaccg acatcctcgg cctcgtcgtc gaagcagtca gcggacagag cctcggtcag
    15241 ttcctcgccg agcgcctctg gcgaccgctc ggcatggtcg atacgagctt cacgctctcc
    15301 gacgcgcagc gcagccgcta tgcccgcgcg tttccgaagt gcccgatcac cggcgacccg
    15361 ctcaccgtgc atcatgcgac cgcggaaatt ccgaaatggg agagcggcgg aggcggcgcc
    15421 gtgtcgacgg cggccgacta tatccgcttc gcgcagatgt tgctcgacgg cggcacgctc
    15481 ggcggccagc gcatcctcgg ccgcaagaca gtcgagctga tgacggcgga ccacctcaca
    15541 ccgtcgatcg tcaaccgcat tacgacgatg gacgcggcct gtgacggcta cggtttcggc
    15601 ctcggcttcg cggtccggcg cggggcgggc ggcgcggcgc tgatgggctc ggccggcgac
    15661 tattactggt ccggcgtcta cggcacctat ttttggatcg acccggccga gcagctcgca
    15721 gcagtcttca tggcggcggc tccgggcatg atccggctgc gctatcgcca gctcatgcgc
    15781 ggcctcgtcc tgcaggcgat cgaggcgtga tcgcgcaatg acgagcgtgc gctggccaga
    15841 ccttgcgggc cgcctcgacg cggatgcgcg cggtcgcgtc cacgtgctgc ccgtgcgcgt
    15901 ctacttcgag gacacggact tctcgggcgt cgtctaccac gcctcctaca tccgctggtg
    15961 cgagcgcggg cggacggact ttcttcgcca ctccggcaac gaccaccgca cgctcatcga
    16021 cgggggcgac ggtcgcgagc cggcagcctt catggtgcgt cggatgcaaa tcgagttcct
    16081 ccgcccggcg cggatcgacg agatcctcga agtcacgacc cgcatcggcg agatcggcgc
    16141 tgccacgctc gagctgcatc aggcggtccg gcggggcgag acgacactgt tcgaggcgaa
    16201 ggtgctcgtc gtgctcgtct cgacggcggg aaagcccttg cggatcggcc cgcggctgag
    16261 ggctgcgctc gcatgaatga cggcgttacc aagatgtcgc ccgagcacac gaccgacgcc
    16321 gcccgcgaac tctgggtatt cggctacggc tcgctgatgt ggcggccggg attcccgcat
    16381 gtcgagacgc atcgggcgcg gctcgcgggg ttcagccgct gcttttgcgt ctactccgtc
    16441 catcaccgcg ggcggaaggg gcggccgggc attgtgctcg ggctcgaccg cgggggcacg
    16501 tgcgacggga tggtctttcg cgttccggac gagcatgcgg cgagcacgct tgcctatctg
    16561 cgcgagcgtg agctcgtcta tggcgtctat cgcgagacct ggcatgaggt gtcgcttctc
    16621 gatgacggag gccgaatcgt gcgtgccgtc acctacattg ccgagcgggc gcacccgagc
    16681 ttcgcgggcc gccagcctgt cggcaagctc gcgcgcctca ttcgcggcgc cgagggggtt
    16741 tccggaccga acatcgagta tctcgtcaac acggtccgac aacttaccct gatgcaagcc
    16801 gatgccggcg agcttcgacg catcctgtcg ctgatttcgc cctatgcggc gacggctggc
    16861 gacggtgccg cgacacgtgc gcgttgcctc gcgatgatgc ggcaccacaa ccatacgcga
    16921 cgccgccgcc cgccgggacc gcgcatcacg ctcgagcgcc tcaatcgctt cctgttccgg
    16981 atgcgcattg cgtcttgatg gcacgaggag atcagcgcag ggcgccgatc aggccgagcc
    17041 agccgatctg cagcggcagg atgaccacga ggccgacagc cgcggcaggg atcagcgccc
    17101 tcagggcgtc acgcgccggc actcgtgcaa tcgcgagacc gaccatcagc ggcacggcct
    17161 cgaacgggaa gaagacggcc tgcaggccgg tgagttcggc gatgagcccc atcttcagcg
    17221 agagaccggt tgcggccgcg atcacatcga tcacggggct cacggtcgcg accgcgccga
    17281 cgatcgtcgc aaccgtcgcc gtcagcgcgg acatcacggc gatggcggag aaaccataga
    17341 ttgccgagcg tccctcgacg gcggcaagac gagcgagctc gcgtccgatg aggtcgctcg
    17401 cgcccgagtg cgtgagcacg gtcgcgacgc cgaggacggc cgccagcgag agcaccgccg
    17461 tcgccttgtt gggatcgaag ctctcgcgcc acgtgacgag gcctgggtag ggcagcaggc
    17521 agacgatacc agcggcgagc gccaccacgc ccggcggcag gcgatgccag aagtctgttg
    17581 cccacagcag cagcgtgacc gcgaggagca atgagaggcg ccgccccgcg ggcgtcagga
    17641 cgggcgcgtc ctcgggcttc ggcagcgcag gcggcggcgc gctgaagaat gcgacgacga
    17701 tcgcgaggaa ggtcgcgccc ttcacgatcc cgagcacggg gagtgtgtag agcagatact
    17761 cgccgtactg gatgatcggg ccatgccgcg cctcgatcgt gccgatggcg atcacgttga
    17821 cgaggtttcc cgtcagcacc gcataggacg tgaggaagtt gcctgcaacc gtcaccagca
    17881 tggcgccgtt gtagccggac gagcctgcca cataaccggc ggccttggcg atggcgccga
    17941 cgatcggaat ggtgagcgcg aggcgcccca tggtcgccgg caccaggaac gagagcacgc
    18001 ccgagccgag gagaatgccg aggagaaagc gcggatagct cgtcacgaag cgctcgacga
    18061 ggccgcgcgc gatccagcgt ccgaggccgg agcgctcggc ggcagcgccg aggatgagtc
    18121 cgccgaggat cagcatggcc gccgtcgacc agaagccgga gagcagcttc agcggaggca
    18181 cgatggcgct cgcgaggccg gccgcgaaga agaggagccc gctcgcccac gccgggatca
    18241 cggccgacgc ccagagcaga accgtcgcgg caacgatgcc gaggccgagc atcgccttgg
    18301 gagaggccga cggtggcgct tgaagcgcga tgagcgcgcc gatcccgacg atgacggcga
    18361 gcagcagcca ctggcggaac gtcagcctac cgtcagccgt catgcgggat cgccccgccg
    18421 ccgcatcagc caatcgaccg ctgcgacgcc atcggacccc gcgaagaccg gattgacgtc
    18481 gagctcctcg atctcggggt gcgcgacggc gagatcggag agcgcgacga ttgcccgcgc
    18541 gacggcatcg cgatcggcgg cggccttgcc gcgcgcgccg tcg
//
LOCUS       DAORNH010000051        18330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2332_length_18330_cov_2.660575, whole genome shotgun sequence.
ACCESSION   DAORNH010000051 DAORNH010000000
VERSION     DAORNH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_18330_cov_2.660575"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..255)
                     /locus_tag="PK264_07380"
     CDS             complement(76..255)
                     /locus_tag="PK264_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75742.1"
                     /translation="MARSTSKRQTAQIVVKISKYCNLRCTYCYEFKDLANKARMSLDK
                     IDRMFATMRRLRVSQ"
     gene            complement(339..668)
                     /locus_tag="PK264_07385"
     CDS             complement(339..668)
                     /locus_tag="PK264_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75743.1"
                     /translation="MSNTIKISAAAIALVSTVGAANAAAPAAQVPGDVAVAAASAPVI
                     SDAARAQILGNEGWQEARHSKTSNTWPCAKCHTKHSKSSFISELKTTLPAGVTALAIF
                     ETLSRAS"
     gene            895..2547
                     /locus_tag="PK264_07390"
     CDS             895..2547
                     /locus_tag="PK264_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD75744.1"
                     /translation="MHLAGATYDELVATFQWRVPQRFNVAEAVVTRHARATPDAPALI
                     VAMDRSDPQVWTFLEVERAANRLANALASLGVRRGDILALHLTQSAETLITHVAASKL
                     GLIVMPLFVLFGPEAVRYRIDDSGAAVLVTTTASLEAVAVDIAACPSLRHIITVDGRG
                     PLGAHSLATLMDAAAHTTVSNNSAAEDPAYLLYTSGTTGNPKGVLHAQRVVIGHLPGV
                     MVPSRFFPQPGDCFWTPADWAWVAGLFDVLMPALYHGVPVVASRSGRFDPEAAFALMA
                     RHKVRNLLLPPTAMRLMRAVPNPRERFAYHVRSLISGGEPVGADMIAWGEDTFGFKLS
                     EVYGQTECNIVVGNNPDIMPVKPGSMGRAMPGHTVAIVDDAGNVLPRGATGIVAVKRP
                     DPVMFLEYWKRPEATREKFRGDWCLLGDVAVEDEDGYLWFQGRDDDIIKSSGYRIGPG
                     EVEDCLARHPAVAIAGVVGTPDPMRGEAVTAFVVAKPGVVPSAELAAEIQAFVKERLA
                     AHESPRKVHFIAEMPLTVTGKLRRVELRKLDAELTPVNSPRA"
     gene            complement(2528..2920)
                     /locus_tag="PK264_07395"
     CDS             complement(2528..2920)
                     /locus_tag="PK264_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75745.1"
                     /translation="MAQAWLQVMGLFLDVMGFGLIAWEWLMAQRAERALLAIEGRRRA
                     EEERQRMMRRHDPNMHPSMQVHMEMVDQMSRRTTAAALETTRTTFSGRRYALVYTGMV
                     FVLAGFILQLAGAWPGCCDFVGITPGAN"
     gene            complement(2946..3338)
                     /locus_tag="PK264_07400"
     CDS             complement(2946..3338)
                     /locus_tag="PK264_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRD75746.1"
                     /translation="MAINAELAELIRDALESLGSISIRRMFSGAGVFCDGLMLALIVG
                     DELYLKADAASIPEFEAEGLEPFRYATKEGERTLSSYWRAPERLLDEPDEMLAWGRKA
                     FEAARRAAKAQARSATKSPRPRRTKAAR"
     gene            complement(3348..4544)
                     /locus_tag="PK264_07405"
     CDS             complement(3348..4544)
                     /locus_tag="PK264_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75747.1"
                     /translation="MSKLTLRWSMLMAAALSAMPALAQRAEDPGALAEGRASTELTRN
                     LNEQTFEIPVDISVAGRRTYRGPMVVTTFRPDGPGPFPLVIYLHGRGADSRHLPARIR
                     SPRIAGYWARRGFAVVIPTRVGYGALREAPDLEDPGYCNDPDYTAMADALAATTVAVA
                     SWARTQAWAEKSRLIIAGSSVGGFGAVVASGRPPEGLLGVVNLAGGAGGRPRLRPGVP
                     CATDRIAEFMTRAGTTARVPSIWVYAENDKFWGTEAPRRWHAAFTKAGGNAEFVMLPP
                     SGEDGHRFATQSYRHWRQNIDRYLERMGIPTPRAKDAPAPSGFAEVEDAARVPLINEK
                     ARTEGYLKFLDADIPRAFAIGPSGAWAWSGGNPGAAERALERCSIHAKADCKLYAVDD
                     AVVWQP"
     gene            complement(4620..5003)
                     /gene="sufA"
                     /locus_tag="PK264_07410"
     CDS             complement(4620..5003)
                     /gene="sufA"
                     /locus_tag="PK264_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HRD75748.1"
                     /translation="MSARKRPAVMTMTPAAVERIRSMMGERPGVAGLRIGVKKGGCAG
                     MEYSMAWAETVGKLDDVIEQDGARVVIDPSAILYLLGTEMDWKVEKLSSQFVFNNPNQ
                     KSACGCGESVELVPASKDRLEGAKA"
     gene            complement(5233..5745)
                     /locus_tag="PK264_07415"
     CDS             complement(5233..5745)
                     /locus_tag="PK264_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRD75749.1"
                     /translation="MSVALDLARARERAPADETLRAEYIQALRLVERLHRLLLDVIKD
                     EFDRQGGAELNSVQALLLYNIGENELTAGELKTRGYYLGSNVSYNLKKLVDMGYIHYK
                     RSETDRRSVRVSLTDKGKAACDVVHGLYRRQLGSIEAVGQVSKDDFQVLNKALIRLER
                     FWSDQIRYRL"
     gene            complement(5885..6448)
                     /locus_tag="PK264_07420"
     CDS             complement(5885..6448)
                     /locus_tag="PK264_07420"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HRD75750.1"
                     /translation="MKPAVVSDVLAVGEVPALEQIAILAKAGFKTLINNQPDGEVDRL
                     PASATVGAEAQRLGLVYRYIPLASRTPPPEQLAAFAAALKELPAPIYACCYSGARSAA
                     GCALILARQSDVDGILAGFAKAGYDVSGIRAFLEKERSGDKVAKPHANGSAEHSKTAT
                     PTATPLPPSYQPVVVLPRASSSGGFAM"
     gene            complement(6494..7579)
                     /locus_tag="PK264_07425"
     CDS             complement(6494..7579)
                     /locus_tag="PK264_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRD75751.1"
                     /translation="MREMIAANSAQWLAIGGLVIGFLFGAIVYRTNFCTMGSISDMMT
                     FGDKRRFRAWLIAAATALIGTQALDHFGIVQLSKSMYLAPTLNWFGNAVGGLMFGFGM
                     VFAGGCASRNLARVGGGDMRALVTLIVIGVFAYMAIGGLLGPVRAFLEQWTAVRLPGA
                     NQGVGTQLAPFIRMPAKTASWIVTGVLATAVVLYAFADSSFRASPVHVLSGIGVGICA
                     VLGWALTGLAFDEFATRPTDPISLTYVRPTGDALEWLQRFTAGPLPGFGVATVFGAVL
                     GSMVMALAMGRFHLVGFSDTGDTVRSLIGAALMGVGGVMALGCTVGQAITGVSTLAIG
                     SFLTFVAILIGGIWGIMALERWLMSEA"
     gene            7719..8816
                     /locus_tag="PK264_07430"
     CDS             7719..8816
                     /locus_tag="PK264_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD75752.1"
                     /translation="MAGPDLSLLSLSSVELRRRIGSKEVSPVEVLEASIARIEEVNPA
                     VNAVTATCFKRARSEAKAAEAAARAGDELGILHGLPTGIKDLEETGGLLTTYGSPLFK
                     DFIPERDNVMVDRVRKAGAIVVAKTNVPEFGAGANSRNPVWGATGNPFNPELNAGGSS
                     GGSAVALATDMLPVCTGSDTGGSLRIPGAVNGVVGFRPSPGFVPHERRSLGWSMISVL
                     GPMGRSVGDTCQLFAVQAGAHPVDPLTWYDGKGNLAEPRPVDLGRLRVAWTEDFGQCP
                     VEGGIRSTLRARMKAMAHLFKDCTEVKLDLGEADRCFDVVRAVSFVQRYQKDYEENPG
                     SLGPNIRANYELGAKMTLADVAWAQAEQTRI"
     gene            8838..9227
                     /locus_tag="PK264_07435"
                     /pseudo
     CDS             8838..9227
                     /locus_tag="PK264_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
     gene            9306..10655
                     /gene="mnmE"
                     /locus_tag="PK264_07440"
     CDS             9306..10655
                     /gene="mnmE"
                     /locus_tag="PK264_07440"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRD75753.1"
                     /translation="MTTSERGAESPRNTIFALSTAPGRAALAVIRLSGPRAGPALAAL
                     VSAMPRPRHASLRRLLHPETGEPVDDALVLWLPGPGTETGEDMAELHIHGGPAIIRAL
                     LRVLGDIDGLRLAEPGEFVRRAFRNGKLDLTAVEGIADLIEAETEGQRRQAMRQASGG
                     LASLYDGWRTRLIEAQALVEAAIDFSDETDVNSDAVARAVVIAGELTSALRRHLDDGR
                     RGEVMRQGLRVVIAGPPNAGKSSLLNMLARREAAIVSEEAGTTRDVIEVFMDLSGLPV
                     ILVDTAGLRDVDAPIEREGIRRSKHQAREADLVVWLSEAAEPIAPPIEALGGRAERVI
                     AVATKIDRSPSLDPSTRGISTMTGEGVETLVQTITDKASSLLGDSESPLITQARHREH
                     VTRALVALEAFLASDMSAPELAAEELRRAAHDLGRISGRVDVEDILDHVFSRFCIGK"
     gene            10757..12631
                     /gene="mnmG"
                     /locus_tag="PK264_07445"
     CDS             10757..12631
                     /gene="mnmG"
                     /locus_tag="PK264_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRD75754.1"
                     /translation="MQRTSFDVIVIGGGHAGCEAAGAAARCGARVALVTHKCSTIGEM
                     SCNPAIGGLGKGHLVREIDALDGYMGRVTDAAGIQFRVLNRSKGPAVQGPRAQADRKL
                     YRRAMQAAIAETQGLHVVEGQAEDFIVGEGRVAGVALADGQRLSARAVVLTTGTFLRG
                     LIHMGETRLPAGRIGEQPAVRLAERIEALGLSLGRLKTGTPARLDGRTIAWGKLEEQA
                     GDDPPVPFSFMTERITTRQVSCHITHTTPATHEIIRVNLGRAPMYSGQIKSTGPRYCP
                     SIEDKVVRFADRSSHQIFLEPEGLDDATVYPNGVSTSLPAEVQRAFLRTMPGLERVEI
                     VRPGYAIEYDYVDPRQLLPSLECRALAGLFLAGQINGTTGYEEAAGQGVVAGLNAARA
                     VVGREPILFSRADSYIGVMIDDLVTRGVTEPYRMFTSRAEFRLRLRADNADQRLTAVG
                     VAAGCVGSERRERYQSKRAALDEAHAMLAARQLTPNEAAAHGIEINRDGRRRSAFELL
                     ALPGITIDRLRVIWPDLPGIPAGICQQIETDARYHPYLIRQNEDVEALKRDEAAVIPA
                     GLDYGAIGGLSTEMRQKLGRVQPVTLAQAARIDGVTPAALMLVAGHLRRLAVPAQPSG
                     "
     gene            12638..13324
                     /gene="rsmG"
                     /locus_tag="PK264_07450"
     CDS             12638..13324
                     /gene="rsmG"
                     /locus_tag="PK264_07450"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRD75755.1"
                     /translation="MSKGAAHAGPILGPEDFIRGFGVSRETIERLETYAALLTQWQKA
                     VNLVAPKTLAEIWHRHFADSAQLVRHAVPKGRIVDLGSGAGFPGLVLAILWRTSLLEG
                     VLSFTLIEADQRKAAFLREAARTTGVPVDIVSTRIEDHATQINVENADVVTARALAPL
                     SRLLELATPLAAPDATFLFLKGRTAADEVGEAAKTWDFQSRLFVSATDPEGRVVALRD
                     VRPKTRGKRP"
     gene            13321..14151
                     /locus_tag="PK264_07455"
     CDS             13321..14151
                     /locus_tag="PK264_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD75756.1"
                     /translation="MSASKAGPGGLRILAVANQKGGVGKTTTAINLGTALAAVGERVL
                     VVDLDPQGNASTGLGIAPETRNATMYDVLSGRSTLAKAMVPTAVPNLTIVSANSDLVG
                     LESELMNDQSRPYKLRDAISALVADNRAKGGAPLDYILIDCPPSLNLLTLNAMAAANA
                     VLVPVQCEFFALEGISQLSETISQIRATLNPALEIQGVVLTMHDARTSFSKEVASSVR
                     SYFGAKVYETMIPRNVRIAEAPSHGKPILLYDYECQGSQAYIKLATEIIERERQYKAA
                     "
     gene            14196..>15046
                     /locus_tag="PK264_07460"
     CDS             14196..>15046
                     /locus_tag="PK264_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRD75757.1"
                     /translation="MGAPLHRSRLGRGLASLIGDPAPTVQKLPPQGEQRLVAIADVRA
                     SKGNPRRTFGEEDLAELADSIRQKGIVQPILVRPDPEAGNGYEIVAGERRWRAAQRAG
                     LHQVPVIVRELSDQETLELAIIENVQRADLNPIEEASGYRDLIERHGYTQERLAEVIG
                     KSRPHLANTLRLLRLPDGVQSLVRDGKLTAGHARALVGRDDADVLADRIVAEGLNVRD
                     VEALVQTVAESPDAVGPVLRRSKDKDADTKAFEKDLSDALGLKAEIKRGSGESGMLVI
                     RFANFDQL"
     assembly_gap    15047..15056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15436..15456
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15463..15993
                     /locus_tag="PK264_07465"
     CDS             15463..15993
                     /locus_tag="PK264_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRD75758.1"
                     /translation="MSPASADDWRSRLGNGFGSYSSGDDTPRASRSRKSGSATSASRS
                     ASRAAADRAPAKRVASEDSAPARKTARVASRSSSSSVSDSYTGIASYYWQPQPVASGG
                     RFNPDAMTAAHKTLPFWTRVRVTHLGNGRSVEVVINDRGPYIAGRIIDLSRAAAGVIG
                     MRDQGIARVRVEVLGR"
     gene            complement(16190..17044)
                     /locus_tag="PK264_07470"
     CDS             complement(16190..17044)
                     /locus_tag="PK264_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRD75759.1"
                     /translation="MSRHDAARGTGSRLLSWVAAAGIGITAIAAPVLAQQVPPKKAAP
                     PAGAPAAAAPAAPAAPQSAWVKLCEKITFKGVDKDKKEVQQEKSICLTHHERLDGQTG
                     MVLISAAVRQIEGQPKNAMMVMVPLGMMLTPGLHVKILSKDLWEKASKNEKIDDSKLK
                     GTKFVFTLCHPAGCTAEIEATPELLTEMKAGGGLVVYAVNAAGQVIAFPIPLVGFAAA
                     LDGTPVDNKMYSEARKQLMDEIRKRQIAAYEAQKAEAAKAAAAFKGAPGAPAPGAPAA
                     APAAPPKK"
     gene            complement(17378..>18330)
                     /locus_tag="PK264_07475"
     CDS             complement(17378..>18330)
                     /locus_tag="PK264_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD75760.1"
                     /translation="RPSGASLLGTEGAPTVLATPAGISIDMGSAPASPADLARAENAR
                     RLAALSEKAGHTLAPTAAPVAASAVAAPVDGTAVRPAAVGMPPATIGPNSLRLAAANG
                     DASAQFDVALRFADGTGVPQDHVQAAEWLQRAASQGFAAAQYRLATAFERGLGVAADP
                     ARARAWYLRAAQSGNVKAMHNLAVLSANREGAAPDYVAAIQWFNEAAERGLADSQFNL
                     AILIESGLGVPKDMALAYKWFALAARSGDREAIRRRDALKAKLSRDELRDAEAAVASW
                     RSKAVDLVTNDVRAAREAWKARAVAQPLPQPRPAAEPVTR"
BASE COUNT         3112 a         5971 c         6158 g         3058 t
ORIGIN      
        1 ggtcgtctat caatccgatt tcggcgcggc ccttgccgag ccctcacccg gacaattcgg
       61 gcgacgctat gtgggctact gggaaacacg caacctgcgc atggtcgcga acatccggtc
      121 gatcttgtcg agtgacatgc gcgccttgtt ggcgaggtcc ttgaactcgt agcaataggt
      181 gcagcggaga ttgcagtact tcgagatttt cacgacgatc tgagccgtct gtcgtttgct
      241 cgtcgagcgg gccatgacga tcactccccg gaaaggctgg tccagtagtc attgacggcg
      301 atggctggcc agggtggaag ccccggccag ccccggcctc aagaggccct cgagagggtc
      361 tcgaagatag cgagcgcggt gacgcccgcc gggagcgtgg tcttcagctc ggagatgaac
      421 gacgacttcg agtgcttggt gtggcacttc gcgcacggcc atgtgttcga cgtcttcgag
      481 tggcgggcct cctgccagcc ctcgttgccg aggatctggg cgcgggccgc gtcggagatg
      541 accggcgcgc tcgcggcggc gacggccacg tcgcccggga cctgcgcagc cggagctgcg
      601 gcattggcgg caccaaccgt tgagacgagc gcgatggcgg cggcggaaat cttgatggtg
      661 ttgctcattg gattgttcct ttgggttgag gtggcttgct gcagtttggt gagcggcgtg
      721 aaatgaactt cgctcatgtc agtattgcgc ggcacagacg gaaaatttac cgcacgatat
      781 gaactttgtt ttcatcgatc gcgtctcagg cagctctcac gcctacgctg gacgtttgcg
      841 gcgccccgag tgatcgtcgg acgcgaacgc catccaacgg atcgaggccc gaccatgcat
      901 cttgccggcg cgacctacga cgagctcgtc gcgacgttcc agtggcgggt tccgcagcgc
      961 ttcaacgtgg ccgaggccgt cgtcacgcgc cacgcccggg caactccgga cgcgcctgcc
     1021 ctcattgtgg ccatggatcg cagcgacccg caggtgtgga cgttcctcga ggtcgagcgc
     1081 gcggccaacc gtctcgccaa cgcgctcgcg tcactcgggg ttcgccgcgg cgacatcctg
     1141 gcgctgcatc tgacgcagtc ggccgagacg ctaatcacgc acgttgcggc gagcaagctc
     1201 ggcctgatcg tgatgccgct gttcgtgctg tttggacccg aggccgtccg ctatcgcatc
     1261 gatgacagcg gcgcggccgt gctcgtcacg acgacggcga gcctcgaggc ggtcgcggtc
     1321 gacatcgcgg catgcccgtc gctccgccac atcatcacgg tcgatggacg agggccgctc
     1381 ggcgcgcact cgctcgcgac actcatggac gccgccgcgc acacgacggt gtcaaacaac
     1441 tccgctgccg aggaccccgc ctatctcctt tatacttccg gcacgaccgg caatccgaaa
     1501 ggcgtcctgc acgcccaacg ggtcgtcatc ggccacctgc ccggcgtgat ggttccaagc
     1561 cgcttctttc cacagccggg ggactgcttc tggacgccgg cggattgggc ctgggtcgcc
     1621 ggtctcttcg atgtgctgat gcccgcgctc tatcacggtg tccctgtcgt cgcctcgcgc
     1681 agcggccgct tcgaccctga agcggccttt gcgctgatgg ctcgccacaa ggtccgcaac
     1741 ctgctgctgc cgccgacggc catgcgcctg atgcgtgccg tgcccaaccc aagagaacgg
     1801 ttcgcctatc atgtccgctc gctcatctcg ggcggcgagc cggtcggcgc cgacatgatc
     1861 gcctggggcg aggacacgtt cggcttcaag ctctcggagg tctatggcca gaccgagtgc
     1921 aacatcgtgg tcggcaacaa tcccgacatc atgcccgtga agccaggctc gatgggccgc
     1981 gccatgcctg gccatacggt ggcgatcgtc gatgatgccg gcaatgtcct accgcgaggc
     2041 gcgaccggca tcgtcgcggt gaagcggccc gatcccgtga tgttcctcga atactggaag
     2101 cggcccgagg cgacgcgcga gaagttccgc ggcgactggt gcctgctcgg cgacgtcgcc
     2161 gtcgaggacg aggatggcta cctctggttc cagggtcgcg acgacgatat catcaagtcg
     2221 tcaggctatc ggatcgggcc gggcgaggtc gaggattgcc tcgcgcggca tccggcggtg
     2281 gcgatcgccg gcgttgtcgg cacgccggac ccaatgcgcg gcgaggcagt gacggcgttc
     2341 gtcgtcgcca agccaggtgt cgtcccgagc gccgaacttg ccgccgaaat tcaggcgttc
     2401 gtcaaggagc ggcttgcagc ccacgagtcg ccgcgcaagg tccacttcat cgccgagatg
     2461 ccgctgaccg tgacgggaaa gctccgccgc gtggagctca gaaagctcga cgcggagctg
     2521 acgcccgtca attcgccccg ggcgtgatgc cgacgaagtc gcagcagccg ggccaggcgc
     2581 cagccagctg caggatgaag cctgcgagaa cgaataccat gccggtgtag acgagtgcgt
     2641 aacggcggcc ggaaaacgtc gtccgtgtcg tctccagcgc tgcggcggtc gtgcgccggc
     2701 tcatctggtc gaccatctcc atgtgcactt gcatcgacgg gtgcatgttc ggatcgtgcc
     2761 gccgcatcat ccgctgccgc tcctcctcgg cgcggcgccg gccctcgatg gcgaggagcg
     2821 cccgctctgc gcgctgtgcc atgagccact cccaggcaat cagaccgaag cccatcacat
     2881 cgagaaacag gcccatcacc tgtagccacg cctgcgccat gtgccgctcc cccgagggtc
     2941 tctgcctatc gagcagcttt cgtgcgccgg ggtcgcggtg atttcgtggc ggaccgcgct
     3001 tgggccttgg cagcacggcg ggctgcctcg aacgccttcc gaccccacgc cagcatctcg
     3061 tcgggttcgt cgagcagccg ctccggagcg cgccagtagg aggacagcgt tcgctcgccc
     3121 tccttcgtcg cataccggaa gggctcgagc ccttcggcct cgaactcggg aatgctggct
     3181 gcatcagctt tcagatagag ctcgtcaccg acgatgaggg cgagcatcag gccatcacag
     3241 aagacacccg ctccgctgaa catccggcgg atcgagatcg agccgaggct ctcgagcgca
     3301 tcgcggatca gctcagcgag ttcggcgttg atggccatgg cggcgtctca cggctgccag
     3361 acgacggcgt cgtcgaccgc atagagcttg cagtcggcct ttgcatggat gctgcatcgc
     3421 tcgagagcac gctcggccgc gccaggattg ccgcctgacc acgcccatgc tccggacggg
     3481 ccgatggcaa aggcgcgcgg gatatcggcg tcgagaaact taaggtagcc ttcggttcgc
     3541 gccttctcgt tgatgagcgg cacgcgagcg gcgtcctcga cctccgcaaa gccgcttgga
     3601 gccggtgcgt ccttggcgcg cggagttggt atgcccatgc gctcgagata gcgatcgatg
     3661 ttttgccgcc agtggcggta gctctgcgtg gcgaagcgat gcccgtcctc gcccgacgga
     3721 ggaagcatga cgaactcggc attgccgccg gccttggtga aggccgcatg ccagcggcga
     3781 ggcgcttcgg tgccccagaa cttgtcgttc tcggcataga cccagatcga gggcacgcgt
     3841 gcggtcgtgc cggcccgcgt catgaattcg gcgatgcggt cggtcgcaca aggcacgccc
     3901 ggcctcagtc tcggccggcc gcctgccccg cccgcaagat tgacgacgcc caacaggccc
     3961 tcgggcgggc gccctgaagc aaccacggcg ccgaagccac cgacagacga tccggcgatg
     4021 atgaggcggc tcttctcggc ccaagcttgt gtccgtgccc aactggcaac ggcaaccgtc
     4081 gtcgccgcga gtgcatcggc catggccgtg tagtcgggat cgttgcagta gcccggatcc
     4141 tcgagatcgg gggcctcacg aagagccccg tacccgactc gcgtggggat cacgacggca
     4201 aaaccccgcc gcgcccagta tccggcgatg cgagggctgc gaatgcgggc cgggaggtgg
     4261 cgactgtcgg cgccacgccc atgcagatag atcaccaagg ggaagggacc tggcccgtcc
     4321 ggccggaatg tggtcacgac catggggccg cgatacgtcc gacgtccggc gaccgatatg
     4381 tcgacgggga tctcgaaggt ctgctcgttc agattgcggg tgagctcggt cgatgcgcgg
     4441 ccctcggcca gtgcgccggg atcctcggct cgctgagcga gcgcaggcat ggcagagagc
     4501 gccgcggcca tcagcatcga ccagcgcaac gtcaacttgc tcatcgcagc agctccggaa
     4561 gtgcagtttc gaaatgcgtc gatgaaatca gcagtcgcaa tggcgcgtca gcaggcgggt
     4621 caggctttgg ccccttcgag ccgatccttg cttgccggga ccagctcgac cgattcgccg
     4681 cagccgcagg cgctcttctg gttgggatta ttgaagacga attgcgatga gagcttctcg
     4741 accttccaat ccatctcggt gccgagcaga tagagaattg ccgacgggtc gatgacgacg
     4801 cgggcgccat cctgctcgat cacgtcgtcg agcttgccga ccgtctcggc ccacgccatg
     4861 gagtactcca tgccggcgca gccgcccttt ttgacgccga tgcggagccc ggcgacgccc
     4921 ggccgctcac ccatcatcga gcgaatacgc tcgacggcgg caggtgtcat ggtcatcacg
     4981 gcggggcgct tgcgggcgga catagggtac ctcgacgggg catccgaaac ggagtgcaaa
     5041 tcggttgccc aatatatagc gcacgcctcg ggaattgcga accagctcga tacgcgccgg
     5101 caaccatttc cgagggccgc accaacagac acggcccggc cgcccggctc cgcctgcccg
     5161 agaaagggag tgcggaaggt gccgggcgtc cgggccggat gcgaggggtc ccgtcggctc
     5221 tgttgacgtg gattacaggc gatagcggat ctggtccgac cagaagcgct cgaggcggat
     5281 cagcgccttg ttgagcacct ggaagtcgtc cttcgacacc tgaccgacag cctcgatgga
     5341 tccgagctgg cggcgataga ggccgtgcac cacatcgcac gcggccttgc ccttgtcggt
     5401 caggctcacc cgcaccgagc gccggtcagt ctccgagcgc ttgtagtgga tgtagcccat
     5461 gtcgacgagc ttcttcaggt tgtaggagac attcgagcca aggtaatagc cgcgggtctt
     5521 gagctcgcca gccgtcagct cgttctcacc gatgttgtag aggaggagcg cttgcacgct
     5581 gttgagctcg gcaccgccct ggcggtcgaa ctcgtccttg atcacatcga ggagcaggcg
     5641 gtgcagacgc tcgacgagcc gcaacgcctg aatgtactcc gcgcggaggg tttcatccgc
     5701 aggcgctcgc tcacgcgcgc gcgccagatc caacgcaaca ctcatgtccc tgcctttcgt
     5761 ccgtttgacg ccccggtgtt tttctttccg ggttgccaga accgtaacag gggaaattaa
     5821 atgttgcgct aatcctcata cttagcaggg agttagcggc cgtcctgttt tccgggctgg
     5881 ccgctcacat tgcgaagccg cccgaggacg acgcccgcgg cagcacgaca accggctgat
     5941 aggatggcgg gagcggcgtg gcggtcggtg tcgccgtctt gctgtgttcg gccgatccgt
     6001 tggcgtgcgg cttggccacc ttgtcgccgc tgcgctcttt ctcgaggaag gcgcggatgc
     6061 ccgagacatc gtaaccggcc ttggcaaagc cggcgaggat accatccaca tcgctttggc
     6121 gggcgaggat cagcgcacac ccggcggccg agcgcgcgcc ggagtagcag caggcataga
     6181 tgggtgccgg cagctccttc agcgctgccg cgaaggccgc aagctgctcg ggcggcggcg
     6241 tgcggctcgc gagcgggata tagcggtaga cgagccccag ccgctgggct tcggcgccga
     6301 ctgtggcgga ggccggcagc cggtcgacct cgccgtccgg ctgattgttg atcagtgtct
     6361 tgaacccggc cttcgccagg atggcgatct gctcgagggc cggcacctcg ccaacagcca
     6421 ggacatcaga cacgacagct ggcttcatgg ataagcgccc gcaacaacac accctggcag
     6481 ccaagcaccg aactcacgct tcggacatca gccaccgctc caacgccatt ataccccaaa
     6541 tcccgccgat gagaatcgca acgaaggtca aaaacgagcc gatggcgagt gtcgatacgc
     6601 cggtgattgc ctggccgacc gtgcagccga gcgccatcac gccgccaacg cccatgaggg
     6661 ctgcaccgat gaggctgcgc accgtatcgc cggtgtcgga gaaaccgacg aggtggaagc
     6721 ggcccatcgc aagagccatc accatcgatc cgagcacggc gccgaatacg gtcgcaacac
     6781 cgaagcccgg caagggaccc gcggtgaagc gctgcagcca ttcgagcgcg tcacccgtcg
     6841 ggcggacata ggtgagcgag atcggatcgg tcggtcgcgt ggcgaactcg tcgaatgcaa
     6901 gcccggtcag cgcccagccg aggacggcgc agatgccgac gccgatgccg gagaggacgt
     6961 gcacgggtga ggcgcggaaa gagctgtcgg cgaaggcata gagcacgacg gcggtggcga
     7021 ggacacccgt cacgatccag ctcgccgtct tggccggcat cctgatgaac ggggcgagct
     7081 gcgtgccgac gccctggttg gcaccgggaa gacggacagc cgtccactgc tcgaggaagg
     7141 cgcggacggg accgagcaac ccgccgatcg ccatgtaggc gaagaccccg atgacgatca
     7201 gcgtcaccag ggcacgcatg tcgccgccgc cgacgcgagc gagattgcgg ctggcgcatc
     7261 ctcccgcgaa caccatgccg aagccgaaca tcagcccgcc gaccgcattg ccgaaccagt
     7321 tgagcgtcgg cgcgaggtac atggacttgg agagctggac gatgccgaag tgatcgagcg
     7381 cctgggtgcc aatgagggcg gtcgcggcgg cgattagcca ggcgcggaac cgccgcttgt
     7441 cgccgaaggt catcatgtct gagatcgagc ccatggtgca gaagttcgtg cgatagacga
     7501 tcgccccgaa caggaagccg ataaccaggc cgccgatcgc gagccattga gctgagttcg
     7561 cagctatcat ctcgcgcatt gcgcggtccc ccaccggtcc gttgctgagc tgtcctcaca
     7621 ttcgcagata tgaatatatc tacgcaaggc ttgcgctcac cttgcgcccg cggcgaagat
     7681 gcacgccgta tcaggctgga tgagaagagg gcgctgccat ggccggtccg gatctgtcgc
     7741 tgctgtcgct gtcgtcggtc gagctccggc gccgcatcgg ctctaaggag gtctcgcccg
     7801 tcgaggtact cgaggcgtcg atcgcgcgca tcgaggaggt gaacccggcg gtcaacgccg
     7861 tgacggcgac gtgcttcaag cgggcgcgga gcgaagcgaa ggcggccgag gccgcggcac
     7921 gtgctggcga cgagctcggc atcctgcatg gcctgccgac cggcatcaag gatctcgagg
     7981 agacgggcgg actgctcacg acttatggct cgccgctctt caaggatttc atccctgagc
     8041 gcgacaacgt catggttgac cgggtgcgca aggctggcgc gattgtcgtc gccaagacca
     8101 acgtccctga gttcggcgcc ggggcgaact cgcgcaatcc ggtctggggc gcaacgggta
     8161 accccttcaa tcccgaactc aacgcgggcg gttcctccgg cggctctgcc gtagcactcg
     8221 cgaccgacat gctgccagtc tgcaccggct ctgatacggg cgggtcgctg cgcatcccgg
     8281 gtgcggtgaa cggcgtcgtt ggcttccgcc cctcgccggg cttcgtgccc cacgagcgcc
     8341 gctcgctcgg ctggtcgatg atctccgtgc tggggcccat ggggcggagc gtcggagata
     8401 cctgtcaact gttcgcggtc caggccgggg cgcatcccgt cgatccgctt acctggtatg
     8461 acggcaaggg taacctcgcc gagccgcgcc ccgtcgatct cggccggctg cgggtggcct
     8521 ggacggagga tttcggccag tgtccggtgg aaggcggcat ccgctccacc ttgcgggcgc
     8581 gcatgaaggc gatggcgcac ctcttcaagg actgtacgga ggtcaagctc gatcttggcg
     8641 aggccgaccg ctgcttcgat gtcgtccgcg cggtgagctt cgtgcagcgc tatcaaaagg
     8701 actatgagga gaaccctggg agcctcggcc cgaacatccg ggcgaactac gaactcggcg
     8761 cgaagatgac gctcgcggac gtggcttggg cccaggccga gcagacgcgc atctaacgcc
     8821 gcttccagga cgtctacgcg aaagagggct acgacctcgt gctcgggccg acggtcggcg
     8881 tcacgccgtt cccgtggacg aagctctacc tcgacgagct cgagggcaag cagctccgca
     8941 actactacca ctggctggcg ccgacctatg tcatcacgct cgtcaccaat ccggcgatct
     9001 cgatcccgtg cggcgtcgac cacaagggca tgccgttcgg gctccaggtc atcggcgcct
     9061 atcagaagga cgccgagctt ctcggggcgg cgctcgccat ggagtgtgca tttggccgcc
     9121 tggccgggct ggaccgtccg cggcctgacc tcgccaagtt ggcaaagccg acacccgcac
     9181 tgaagtcgat cgtcacacat ccgccaagac gctcggagat gggctgagcg ggacggaccg
     9241 gctctgtttc acgtgaaaca cgcggtacac catccactcg gttgctcgac ccgaaccagg
     9301 ctccaatgac gacgtccgag cgcggcgcag agagcccgcg caacaccata ttcgcgctgt
     9361 cgacggcgcc cggtcgggcg gcactggccg tcatccgact ttccggaccg agggctggac
     9421 cggcgctcgc tgcattggtc tcggcgatgc cgcggccgcg ccacgcgagc ctccgccgcc
     9481 tcctccaccc cgaaacgggg gagccggtcg acgacgctct ggtcctctgg ctgccgggac
     9541 cagggaccga gactggcgag gacatggccg agctgcacat ccacggcggt ccagcgatca
     9601 ttcgggcgtt gctgcgggtc cttggcgaca tcgacggttt gcggctcgcc gagccaggcg
     9661 agtttgtccg acgcgcattc cgaaatggca agcttgacct gaccgccgtc gagggaattg
     9721 ccgacctcat cgaggctgag acggagggac agcggcgcca ggcgatgcgc caagcgtccg
     9781 gcgggctcgc gagcctctat gatggctggc ggacccggct catcgaggca caagccctgg
     9841 tcgaggcggc catcgatttc tcggatgaaa ccgacgtcaa cagcgacgcc gttgcccgtg
     9901 ctgtggtcat tgcgggcgaa ttgacctcag cactgcgccg tcatctcgat gacggccggc
     9961 gcggcgaggt gatgcggcag gggctgcggg tggtgatcgc cggaccgccg aatgccggca
    10021 agtcgagtct gctcaatatg ctggcccgcc gtgaagccgc gatcgtctcg gaggaagccg
    10081 gcaccacccg cgacgtcatc gaggtcttta tggacctctc gggtctgccg gtcattctgg
    10141 tcgacacagc gggcctgcga gacgtggacg cccccattga gcgggaaggc atccggcgca
    10201 gcaagcatca agctcgggaa gcggacctcg tcgtttggct gagcgaggca gcggagccaa
    10261 tcgctccgcc catagaggcg ttgggtgggc gggctgaacg ggtcatcgcc gttgcgacga
    10321 agatcgatcg atcgccgtcg ctggacccgt cgacccgcgg gatctcgacc atgacgggcg
    10381 agggagtcga gaccctggtc cagacgatca ccgacaaggc ctcgtcattg cttggtgaca
    10441 gtgagagccc cctcatcacg caggcccgcc atcgcgagca cgtgacgagg gctttggtag
    10501 cgctcgaggc atttctcgcg tccgacatgt ccgctccgga gttagccgcc gaggaattgc
    10561 gccgagccgc ccacgatctg ggccgcatct cgggccgtgt cgatgtcgag gacatcctgg
    10621 accacgtctt ctcacggttc tgcatcggca aatagcgcgc ccggtcgttt cacgtgaaac
    10681 gatcgcgccc gctctccggc tttgacccgc gaagtcgcaa tcgggtatgg tccggccgga
    10741 ttccagggac cccaaaatgc aacggacctc attcgatgtg atcgtgatcg gcggtgggca
    10801 tgccggctgc gaggccgcgg gtgcggcggc gcgttgcggc gcccgcgtgg cgctggtcac
    10861 ccacaagtgc tcgacgatcg gtgagatgtc atgcaacccg gcgatcgggg ggctcggcaa
    10921 aggccacctc gtccgtgaga tcgatgcgct cgatggatac atgggccgcg tgacggacgc
    10981 cgcgggcatc cagttccgcg tgctcaaccg atcaaagggc cctgccgtgc aggggccgcg
    11041 tgcgcaggcg gaccgcaagc tttatcgccg ggcgatgcag gcggcgatcg cggagaccca
    11101 aggtctccat gtcgtcgaag gacaggctga agatttcatc gtcggtgagg gccgagtcgc
    11161 gggtgtggct ctcgcggacg ggcagcgcct gagtgcccgg gccgttgtcc tcacgaccgg
    11221 tacctttctg cggggcctca tccatatggg cgagaccagg ttgcccgccg gccgaatcgg
    11281 cgagcagccc gccgtccgcc tcgccgaaag gatcgaggcg ctgggtctct ctctcgggcg
    11341 cctcaagacc gggacgcccg ctcgcctcga cggacgcacc attgcctggg gcaaattgga
    11401 ggagcaagcg ggcgatgatc cgccggtgcc gttctccttc atgacggagc gaatcacgac
    11461 gcgccaagtc tcctgccaca tcacccacac gacccccgcg acacacgaga tcatcagggt
    11521 gaacttgggc cgcgcgccga tgtactcggg ccagatcaag agcacaggcc ctcgctactg
    11581 cccctcgatc gaggacaagg tggtgcgctt cgccgaccgt tcctcgcacc agatctttct
    11641 cgagccggaa gggctcgacg atgccaccgt ctatccgaac ggggtctcga cctcgctgcc
    11701 ggcggaggtc cagcgcgcgt tcctgcgaac gatgccgggc ctcgagcgtg tcgagattgt
    11761 gaggccgggg tacgccattg agtatgacta cgtcgacccg cgccagctgc tgccgtcgct
    11821 cgagtgccgg gctctcgcgg ggctgtttct cgccgggcag atcaacggaa cgacaggcta
    11881 cgaggaggct gccggccaag gcgtcgtcgc gggccttaat gcggcgcgag ctgtggtcgg
    11941 ccgcgagccg atcctgttct cgcgggccga cagctatatc ggcgtgatga tcgacgacct
    12001 ggtaacgcgg ggggtgacgg agccctatcg catgttcacg tcccgggcgg agttcaggct
    12061 aaggctcagg gccgacaatg ccgatcagag gctgacggcg gttggggtgg cggcaggctg
    12121 tgtcgggtcc gaaaggaggg agaggtacca gtccaagcgg gccgcactgg atgaggcaca
    12181 cgcgatgctc gcggcccgac agctgacacc gaacgaggct gcggcccacg gcatcgagat
    12241 aaaccgcgat ggccgacgcc ggtcggcatt cgagttgctt gcgctgcccg gcatcacgat
    12301 cgatcgcctt cgcgtcatct ggccggatct cccgggcatc ccggccggca tttgccaaca
    12361 gatcgagacc gatgccaggt atcacccgta tttgatccga cagaacgagg acgtcgaagc
    12421 cctcaagcga gatgaggcgg cggtcattcc ggccgggctc gactatgggg cgatcggcgg
    12481 cctgtcgacc gaaatgcgcc agaagctagg gcgcgtgcag ccggtgactc tcgcccaagc
    12541 ggcgcggatc gacggggtca cgccggcggc gctgatgctg gtggccgggc atctgcggcg
    12601 cctcgcggtc ccggcgcagc caagcgggtg acatcagatg tcgaaagggg ccgctcatgc
    12661 cgggccgatc ctgggaccgg aggacttcat ccggggcttc ggtgtttcac gtgaaacgat
    12721 cgagcggctg gagacttatg cggccctgct gacccagtgg cagaaggccg tgaacctcgt
    12781 cgccccgaag acactcgccg agatctggca tcggcacttc gccgacagcg cacaactcgt
    12841 ccgacacgcg gtgccgaagg gtcggattgt ggatctcggc agcggcgccg gctttccagg
    12901 gctcgtcctc gccattcttt ggcggacgtc gctccttgaa ggcgttcttt cgttcactct
    12961 catcgaggca gaccagcgca aggccgcatt cctacgggag gccgctcgaa cgaccggagt
    13021 gcctgtggat atcgtctcga cgcgaatcga ggatcatgcg actcagatta acgtcgagaa
    13081 tgccgatgtt gtcaccgcgc gagcactggc gccgctcagc cgcctcctcg agctggcgac
    13141 gccgctggcc gcccccgacg cgacgttcct gttcctcaag ggccggacgg cggcggacga
    13201 ggtcggcgag gccgcaaaga cgtgggactt ccagtcgcgt ctgttcgtga gtgcaacaga
    13261 tcccgagggc cgagtcgtgg ccctccgtga cgtgagaccc aaaacgaggg gtaagcgacc
    13321 gtgagcgcaa gcaaggcagg gcccggcggt cttcggatcc tcgccgtcgc caatcagaag
    13381 ggcggtgtcg gcaagacgac gacggcgatc aacctcggca ccgcactcgc ggccgtcggc
    13441 gagcgggtgc tggtcgtcga cctcgatccg cagggcaacg caagcacggg cctcggcatc
    13501 gcgccggaga cccgcaacgc gaccatgtac gatgtactga gtgggcgctc gacgctcgcc
    13561 aaggccatgg tgccgaccgc ggtaccgaac ctgaccatcg tctccgccaa cagcgacctc
    13621 gtcgggctcg agtcggagct gatgaacgat cagagcaggc cctacaagct gcgcgacgcg
    13681 atttcggcgc tcgtcgccga caatcgcgcc aagggcggcg cgccactcga ctacatactg
    13741 atcgactgcc cgccgtcctt gaacctgctc actctcaacg ccatggcagc cgccaacgcc
    13801 gtgctggtgc cggtgcagtg cgagttcttc gccctcgagg ggatctcgca gctgagcgag
    13861 accatcagcc agatccgggc gacgctaaat ccggcgctcg agatccaggg cgtggtgctc
    13921 accatgcatg atgcgcggac gagcttctcg aaggaggtcg cgagcagcgt gcgctcgtac
    13981 ttcggcgcca aggtctatga gacgatgatc ccccgaaacg tgcgcatcgc cgaggcccct
    14041 tcgcacggca agccgatcct gctctacgac tacgaatgtc agggcagcca agcctacatc
    14101 aagctcgcca ccgagatcat cgagcgcgag cggcaataca aggcggcgtg acggactgag
    14161 gtcgaaactt cggagatcgg gagcctcgac attccatggg tgcaccactg cacaggagcc
    14221 gccttgggcg tggcctcgcc tcactgatcg gcgatccggc gccgaccgtg cagaagctgc
    14281 caccgcaggg tgagcagcgg ctcgtcgcca ttgccgatgt gcgcgcgagc aagggcaatc
    14341 cgcgccgcac gttcggtgag gaggatctcg ccgagctcgc cgactcgatc cgccagaagg
    14401 ggatcgtgca gccgatcctc gtgcgacccg atccggaggc cggcaacggc tacgagatcg
    14461 tcgcaggcga gcgccgctgg cgggcggcgc agcgcgcggg gctgcatcag gtccccgtga
    14521 tcgtgcgcga gttgagcgat caggagacgc tcgagctcgc catcatcgag aacgtgcagc
    14581 gcgccgatct caatccgatc gaagaggcga gcggctatcg cgacctcatc gagcgtcacg
    14641 gctacacgca ggagcgactg gcagaggtca tcggtaagag ccgcccgcat ctcgccaaca
    14701 cgttgcggct gctcagactg ccggacggcg tccagtcgct ggtgcgcgac ggcaagctga
    14761 ccgcaggtca tgcccgggcg ctggtagggc gggatgatgc cgacgtgctc gcggatcgca
    14821 tcgtcgccga gggtctcaac gtcagggacg tcgaggcact cgtccagacc gtggccgagt
    14881 cgcccgacgc ggtggggccg gtattgcgcc gctccaagga caaggacgcg gataccaaag
    14941 cattcgagaa ggatctctcg gatgcgctcg gattgaaggc cgagatcaag cgcggatcag
    15001 gtgagagcgg catgctcgtc atccgtttcg ccaacttcga ccagctnnnn nnnnnnatat
    15061 ccggcagcgg ctcgggggct cgacggagta gttgttcgag acgtcggacc cgcatctgga
    15121 aaggccatgc cagccatgca cttggccgtt tgggttaatt tttcgggttg acggatcagc
    15181 gtcgacctgc tcatgtccgg ccgccaggac acgggcgact gcccgtacaa ctcgtcatgc
    15241 ggcatgagcg tcctggcggt gagatggggt tgcatcgata ggcatctccg gggggcggga
    15301 ctgatccttt gcccacgcaa cgctttttgg ggtcggcgcg ctcagcccgg ccagtgcggt
    15361 agcgttgtcc ctcatccgcc gcgtgttggt taggagagtg acatgctgaa taagcttctt
    15421 atcggcgggg tgagcnnnnn nnnnnnnnnn nnnnnngtgg tgatgtcgcc ggcaagcgcg
    15481 gatgactggc gctccaggct cggcaacggc tttggctcct actcgagcgg tgacgatacg
    15541 ccccgtgcct ctcgcagccg caagtcaggc tcggccacat cggcctcgcg atcggcctcg
    15601 cgggcagcag cagaccgagc gccggccaag cgcgtcgctt cggaggattc ggcccctgcc
    15661 cgcaagacgg cgcgcgtggc gagtcgctca tcgtcatcct cggtgtccga cagctacacg
    15721 ggcatcgcct cgtactactg gcagccgcag cccgtcgctt ccggcggccg gttcaatccg
    15781 gatgccatga cggctgccca caagacgctg ccgttctgga cgcgcgtgcg tgtcacgcat
    15841 ctcggcaacg gtcgctcggt cgaggtggtg atcaatgatc gcggccccta catcgccggg
    15901 cggatcatcg atctgtcgcg cgcggcggcc ggcgtgatcg gcatgcgcga tcagggtatc
    15961 gcccgggtgc gcgtcgaagt tctcggccgg taaggacgat cagtgatccg agattggcag
    16021 cccgtgccct gatgtcggga tgcgggcggc ccatcacccg ctgggggtga gcgaccggtt
    16081 tgtctgatcc tgcagggttc ccgacgtgtt ttcggccgcg cagtccctca ggattgcgcg
    16141 gccgtttcaa tagtgctgcg tccaaatccg cttcagcggt ggagcgccac tactttttcg
    16201 gcggtgcggc cggagcggca gcaggcgcgc ccggagccgg agccccggga gctcccttga
    16261 aggcggcggc ggccttcgcg gcttcggcct tctgcgcttc gtaggcggcg atctggcgct
    16321 tgcggatctc gtccatcagc tgcttgcgcg cttcgctgta catcttgttg tcgacgggcg
    16381 tgccatcgag cgccgcggcg aacccgacca gagggatcgg gaaggcaatg acctggccgg
    16441 cagcgttcac ggcatagacc acgagcccgc cgcctgcctt catctcagtg agcagctccg
    16501 gcgtcgcctc gatctcggcc gtgcagccgg ccggatggca gagcgtgaag acgaacttcg
    16561 tgcccttcaa cttgctgtcg tcgatcttct cgttctttga ggccttttcc cagagatcct
    16621 tcgagaggat cttcacgtgc aggcccggcg tcagcatcat gccgagcggc accatcacca
    16681 tcatggcatt cttgggctgg ccctcgatct ggcggacggc cgccgagata agcaccatgc
    16741 cggtctggcc gtcgagccgc tcgtggtggg tgaggcagat gctcttctcc tgctgcacct
    16801 ccttcttgtc cttgtcgacg cccttgaagg tgatcttctc gcacagcttc acccaggcgc
    16861 tctgtggtgc cgccggcgca gccggggccg cagcggctgg tgcaccagcg ggaggtgctg
    16921 ccttcttcgg cgggacctgc tgagcgagca ccggcgcggc gatcgccgta ataccgatgc
    16981 cagctgccgc aacccaagag agcagccgcg agcccgtgcc gcgcgccgcg tcatggcgag
    17041 acatgatcct ctcctccatg cgaatgcacc tttcatgatc gtgagcggcc cggccgctat
    17101 ccccaagaac ctcgtggctg gccatatcgg ccaacagctc ccctgccgat cgcgttgaat
    17161 tcgaacgatc ggggtgcgcg agcgggtgca ggaaatgacc tggattgcgg cattcccgtg
    17221 gccacgggct atgaccgatc gtgggtatcg aggtgacaag ccccgatcgt ggtaaatggg
    17281 caacggccca tgcgtcaatc aggactgcag taaagcaaag ggcggggcgc catagccccc
    17341 cgcccgattg aaccgcttcg ttgagcgacc gacgtgatca gcgtgtcacc ggctccgccg
    17401 ccgggcgcgg ttgcggcagc ggctgggcga cagcgcgcgc cttccaggcc tcgcgggcag
    17461 cgcggacgtc attggtgacg aggtctactg ccttggagcg ccagctggcg accgccgcct
    17521 cggcatcgcg cagctcatcg cgggagagct tggccttgag cgcgtcgcgg cggcggatcg
    17581 cctcacgatc gccgctacgg gcggcaagcg cgaaccactt gtaggcgagc gccatgtcct
    17641 tcgggactcc gaggccactt tcgatcagga tggcgagatt gaactggctg tcggcgaggc
    17701 cccgctcggc cgcctcgttg aaccactgga tcgctgcgac gtagtcgggc gctgcgccct
    17761 ctcgattggc gctgagcacg gctagattat gcatggcctt cacgttgccg gactgggcgg
    17821 cacgcagata ccaagcccga gcccgggccg gatcggcagc aacgccaaga ccgcgctcga
    17881 aggccgtcgc caggcggtac tgggcggcgg caaaaccctg cgaggcggcg cgctgcaacc
    17941 actcggcggc ctggacgtga tcctgcggca caccggtgcc atcggcaaat ctgagtgcaa
    18001 cgtcgaactg agcggaggcg tcgccattgg ctgccgcgag ccgcagcgag ttggggccta
    18061 tggtcgccgg cggcattccg accgccgcag ggcggacggc agttccgtca acgggagctg
    18121 caaccgccga ggcagcgact ggggccgccg tcggcgcgag cgtatgcccg gccttctccg
    18181 agagggcggc gaggcgacgc gcattctcag cgcgggcgag atcggcggga gaggccggcg
    18241 ccgagcccat gtcgatcgag ataccggcgg gggtcgcgag gactgtcggg gcgccttccg
    18301 tgccgagcag cgaggcgcca ctcggacgcg
//
LOCUS       DAORNH010000052        18186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2361_length_18186_cov_2.795433, whole genome shotgun sequence.
ACCESSION   DAORNH010000052 DAORNH010000000
VERSION     DAORNH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18186
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_18186_cov_2.795433"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>287
                     /locus_tag="PK264_07480"
     CDS             <1..>287
                     /locus_tag="PK264_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75761.1"
                     /translation="LDRAERACRAELAELDDLIARGASARDQIAVLAEQGACAAVMEK
                     AAASLAGMSSAKGKAKTGPTTKPAAPGELKLLATFEGHSNVIQAIAFSPDG"
     assembly_gap    288..332
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <333..1174
                     /locus_tag="PK264_07485"
     CDS             <333..1174
                     /locus_tag="PK264_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75762.1"
                     /translation="DLERNRHIATFAPNCGKVQAITFSPDGKLIAAGCYGNVSFYDWQ
                     SKTPRSDLKSLNGYVFSLAFLGNSEKAIGMIHGGSSYSIFVAPAPASPPLISQPPNAD
                     RNIFYVGNSVLGVAHAPNGRMTAVASRDGGTMLFDPGTGKRLRAFSRPGHDAYAVGFT
                     PDSEILITGESDYKIVVRDIGSGQIKSLLTGHKVRPVGLAVSSNGSRLASGDHDGHLM
                     VWDLQSGATTPLASVKAHGKSVFAVAISPKSRIIATSGAEEYQSGKGHVGHSIKLWAM
                     PGP"
     gene            1259..>1779
                     /locus_tag="PK264_07490"
     CDS             1259..>1779
                     /locus_tag="PK264_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HRD75763.1"
                     /translation="MSLEITLSLAAIFLLLMLSAFFNGSETALTAASRARMHTLEQEG
                     NRKAALISRLLANPERMIGAVLLGNTLVDVLAAALASNLATHLVGDIGVAYATAIMTV
                     LIVIFAAVLPKTYALAYSDRIALAVAPVMRWVITILTPLTAAIQFVVRLFLKLTPTKK
                     DDDANILATHEELR"
     assembly_gap    1780..1915
                     /estimated_length=136
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1916..2570
                     /locus_tag="PK264_07495"
     CDS             <1916..2570
                     /locus_tag="PK264_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HRD75764.1"
                     /translation="PPQKIIEDALKSQYSRLPVWKEEPDNIVGVLHTKDLLAALARAG
                     YDPAKIDIMSFAADPWFIPDGTSVKDQLNQFLKRKAQLALVVDEYGEVQGLITLEDIL
                     EEIVGQISDEHDTGDVSLRQQPDGSVIADGGLAIRDLNRQMDWDLPDEEATTLAGLVI
                     HEAQTIPESGQTFTFHGFRFEIIRKSRNRITAMRVRPVKAMPQRSRPTAEIDPPYSG"
     gene            2575..>3819
                     /locus_tag="PK264_07500"
     CDS             2575..>3819
                     /locus_tag="PK264_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD75765.1"
                     /translation="MMETSAFPYRRSADQDRPTPARHDVVIVGAGPVGLVLAIDLAQR
                     GYRPIVLDRKHGLSDGSRAICWSKRTLEIMGRLGLANRLVARGVTWKTGKVFAGERQL
                     YEFDLLPEPGHRQPAFINLQQFLFEADCFAEAERLGIEVRSGHEVTRVAAAAEHARIE
                     IATPDGPYTLEARYLVAADGARSTVRHSLGLGFEGQVFNDQFLICDVKLELDRPTERW
                     FWFDPPFNRGQSALLHRQADNVWRLDFQIGPEADRDEEMRPENVARRVRAMLGDTPFS
                     FEWISCYRFQCRRLARFRHGPILFVGDAAHQVSPFGARGGNSGVQDADNLAWKLDLVL
                     CGVSPDALLDTYDSERRQAADENIAASTRSTDFISPKGPGATLLRDSVLALAEREPFA
                     RRLLNSGRLSTPTTYDGSPLNSP"
     assembly_gap    3820..3829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3830..4175
                     /locus_tag="PK264_07505"
     CDS             <3830..4175
                     /locus_tag="PK264_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD75766.1"
                     /translation="GLANGPAALRPGAPALDAPLPRNEGGGYLLDRLDKFVLLSVGES
                     VAAGAAPSSMPIPGVEQLTLASPDVVRRYGAGAGQAHYLIRPDQHIAARWSHAAPLDL
                     TTTCERARGRSI"
     gene            4211..5335
                     /locus_tag="PK264_07510"
     CDS             4211..5335
                     /locus_tag="PK264_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HRD75767.1"
                     /translation="MKTRAAVIRRMEQPRPYTVSRPLAIEELDLDPPGEGEVLLRIRA
                     AGLCHSDLSTINGDRPRQMPMALGHEAAGEVMELGPGVRDLAKGDHVILVFVPSCGHC
                     NPCAEGRPALCEPGFKSNGAGTLVSGATRLSLKGERIWHHLGCSAFAEYAVVSRQSVV
                     KIDRSLPFEEAALFGCAVITGAGAVINTGRLQAGETAAVIGLGGVGLMAMLAAKVAGA
                     RQIIAVDMLDDKLKLAKQLGATHTVNARDPKAIEAIRDLSGGGVDFGFEMAGSAKALE
                     LAYKVTRRGGTTITASLPHPTHTMALPVTNLVAEERTVKGSYVGSCVPSRDIPRFIAL
                     YQQGLLPVDRLMSERIALDDINMGFERLAEGATVRQLLIP"
     gene            5437..7233
                     /locus_tag="PK264_07515"
     CDS             5437..7233
                     /locus_tag="PK264_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRD75768.1"
                     /translation="MAATINMAAYSDALVVLGTAGVVVPLVKRFGISPVLAYLGAGAL
                     LGPLALGSFIGSFPILYWFTVVDAQNVAGIADLGVVFLLFLIGLELSYERLYAMRRLV
                     FGLGGLQVLATTVVLSAVIALAGAKGTAAFILGSCLALSSTAIVLEVLSSQGRMPTSA
                     GRSSFAVLLAQDLAVIPILMFVSIVGSNGGSSVLGTIGATIYHAVIAVAVIVLVGRTM
                     LRPLFRIVVATQSSELFIATILFVIVATGVVAAAAELSMALGAFVAGLLLAETEYRKA
                     IEATIEPFKGLLLGIFFFTVGMSIDPRELAREPLLLFLSVIGLIGLKAAIVLGLARLF
                     RLTWPTSIETALLLGPGGEFAFVGIGLAATLGVVAKSTASFALAVTALTMVLIPALSY
                     LGQRFGRRMEPPKPLDAALLAVPEQKDHHAIVVGYGRVGRVVSELLEEHSIAYTAVDH
                     DPVSITRAKADGHPVYYGDATSEAFLKACGLMECSGVIITIHDQAMLDEIVLLIRRLR
                     PDIMIVARAKDAVHAGHLYAIGVTDAVPETIEASLQLSEAALVGLGRQAGPVIASIHE
                     HRDRVRQVLQQAASKAGAPQSRALRQKLPGKL"
     gene            complement(7243..7455)
                     /locus_tag="PK264_07520"
     CDS             complement(7243..7455)
                     /locus_tag="PK264_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HRD75769.1"
                     /translation="MTKTDLAKLQAYLRKTFGTPNLEVRARPKKTDSAEVYIGDEFLA
                     TVTLDTEDGSRDWQLQMAILEMDLDG"
     gene            7606..>7857
                     /locus_tag="PK264_07525"
     CDS             7606..>7857
                     /locus_tag="PK264_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD75770.1"
                     /translation="MTDDALLSAFLEMMAAERGAARNTVEAYRRDLEDFTAALHRSGH
                     TIVTADCARINGYLRDATAAGLSAASRARRLSAIRQLYRF"
     assembly_gap    7858..7912
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7913..8543
                     /locus_tag="PK264_07530"
     CDS             <7913..8543
                     /locus_tag="PK264_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD75771.1"
                     /translation="KERALPRTLSVAEVDRLLDTARNRTGAAVGIERIRALRFRALIE
                     MLYATGMRVSELVSLPRNVLDGDGRMLVVKGKGGRERLVPLNQTARAALDDFLAIGTG
                     GDDLARMVRTRWLFPSKSAEGHLTRQRVGQDLKALAFEAGLDPERVSPHVLRHAFASH
                     LLDRGADLRSVQQLLGHADISTTEIYTHVLEERLKALVERHHPLAGPRS"
     gene            complement(8834..9850)
                     /locus_tag="PK264_07535"
     CDS             complement(8834..9850)
                     /locus_tag="PK264_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRD75772.1"
                     /translation="MKRPAPTSDPNKPRLTTSAMIASIAGEGQKTKPAPFGHALVAAA
                     KADPKIVGMTADLGKYTDLHIFKEAFPDRFYQMGMAEQLLLGAASGMAAEGFTPFATT
                     YAVFATRRAYDFIHQTIAEEDRNVKIVCALPGLTSGYGPSHQAAEDVALMRAMPNMTV
                     IDPCDALETEQATHAIAAHKGPVYMRLLRGQVPLVLDEYGYTFELGKAKLICDGAEVL
                     IISSGLLTMRALEASKELAKDKINAAVLHVPTIKPLDTAMIVEACKKPGRLVVVAENH
                     SVIGGLGEAVAATLMRAKVHPAFEHIGLPDAFLDAGALPTLHDRYGISTTAITARIKA
                     ALGK"
     gene            complement(9847..10692)
                     /locus_tag="PK264_07540"
     CDS             complement(9847..10692)
                     /locus_tag="PK264_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRD75773.1"
                     /translation="MAKPTALPNKTLADRAYHIRRNAVRMGEVQGQGYIAQALDIADV
                     LAVAYFHAMKYRADDPAWEGRDRFLLSNGHYAIALYAALIEAGIVPESELETYGSDDS
                     RLPMSGMASYTPGMEMSGGSLGLGLSIAVGMCLGLKRKNSDARVYTLFSDGELDEGSK
                     WEAIMSAAHYKLDNLIAVVDINNQQADGPSTGVLSFEPLVDKMQAFGWFTQRVDGNDL
                     DAVVKAFDAAKSNNGGRPSIIIADTLMGKGVPFLEARDKNHFIRVDAHEWQLALKALD
                     DGRHA"
     gene            complement(10685..11437)
                     /locus_tag="PK264_07545"
     CDS             complement(10685..11437)
                     /locus_tag="PK264_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75774.1"
                     /translation="MLLKGKTAVISGAAGKRGIGLATAKLFADHGARVAILDIDGKAA
                     IEAAGSLGKDHIGLACDVTKLDQCKAAVDAAVKAFGHVDILINNAGITQPVKLMEIDA
                     ASYDRILDVNLRGTLYLSQATIPHMRSRKSGSIACTSSVSGQRGGGIFGGPHYSAAKA
                     GILGLAKAMARELGPDGIRVNCVTPGLIQTDITGDKLTPKMKTEIAAGIPLNRLGEVK
                     DVAGCFLFLASDLSSYITGAVIDVNGGMLIHG"
     gene            11610..12506
                     /locus_tag="PK264_07550"
     CDS             11610..12506
                     /locus_tag="PK264_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD75775.1"
                     /translation="MIGSVSLKGIRAFEAVARLGSFRAAATEMKLTSSAVSHAVIDLE
                     RDLGIGLIDRQKRGGRLTERGEAFYAHVRIAFDQLRLGLDEASQRMRRMFRLHAAPTF
                     AAGWLSPRLPRFLALHPEIEVRLSAGTDYSSFSNNDFDADIVYGPVRATNVTIVPIIE
                     ETIAPMCAPSIAALITTPADLRRQVLIQSETKMVRWSHWFEKNGEPSPNIHGIRFDRS
                     FLAIAAAADGLGVALESTLLAERELASGRLVMPLAGRAKDITYVGHSLVFPKLAKPNK
                     LVGVFAEWLKQEIGDSKMTTAE"
     gene            13022..14269
                     /locus_tag="PK264_07555"
     CDS             13022..14269
                     /locus_tag="PK264_07555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75776.1"
                     /translation="MPRGPWLPPALTKPRYRIYAVGMGISLMGYWVQTIALSWLVYRL
                     TDSVFILGLAGFMQQIPSLVVPPIAGMLADRLPRVKLVLILNLVLGLLALTLAILVWS
                     GVKDVRIYLAMAFLTGCVAASEAPNRQSLVGAIVEEPALLPSAIAFHSVIFNTGRMVG
                     PTVAGLLLLVMSEAWCFFINAISYVAVLIAFRMIGLKDGARAKSQESFIAMLRSTAAR
                     VGELPVARYFLPLVATAGLLAAPYQALMPSYVDDVLGGGTGTLGLLVGAAGAGALSAA
                     IYLSLQTGGRAQLRLVQLAPFSLGLAVIGFGLSRSLPLSCLCLYAIGASIMCTTATTN
                     TLLQLSVDPGWRARVIGIFQMSFAGVNPVGSLIAGLVAARLGIGPTFVLNGALILLVG
                     LLMRWRLKRSPDAARELERVVGG"
     gene            14406..14636
                     /locus_tag="PK264_07560"
     CDS             14406..14636
                     /locus_tag="PK264_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HRD75777.1"
                     /translation="MNRFERLFGLRGEARVRFSDGEYQVISPGDFVTCAVTGKPIPLP
                     DLKYWNVDLQEAYASSEISFKRALELRAARRR"
     gene            14651..15379
                     /locus_tag="PK264_07565"
     CDS             14651..15379
                     /locus_tag="PK264_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRD75778.1"
                     /translation="MAPRKPKIAAAELDDRREVGRLHELHPRQVFLAMSTAGLSPGRS
                     GNVSARHADGMLITPSGVAYDTLDPADIVHVRADGRVDPGQLKPSSEWRFHLATYAAR
                     PDACGIVHCHSLSATALACTKKPIPAFHYMVAAAGGADIPVVPYATFGTEELSRHVVA
                     GLKDRNACLMANHGQIAVAASVGAALELAHEVEVLAEMYIKVLAIGAPHILDAAEMAR
                     MLTLFRSYGQRAQDGAKPEGKAKA"
     gene            complement(15385..16557)
                     /locus_tag="PK264_07570"
     CDS             complement(15385..16557)
                     /locus_tag="PK264_07570"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HRD75779.1"
                     /translation="MTIAAIILAGGQGTRAGPVPKQYQPIGGVPLLRRTVAAFLAEPR
                     VEQVAVVCPRVDAARVAAILAGLDRDSRLMAPVTGGATRQASGLAGLEALAASVLPPE
                     TVLIHDAARPFVTRALLDRIIGGLDRHPAVIPGLAVADTLKIVDAIGRITGTQPRDKL
                     WRAQTPQAFRFAEILVAHRKAATAGRHDFTDDAALAEWAGLPVGIVEGSEANRKLTTA
                     EDLAMADDMLSREGEVRIGQGFDVHAFKPGDHVWLCGVRIPHACALEGHSDADVGLHA
                     LTDALLGAIGDGDIGQHFPPSDERWRGAASDQFLADAARRVRERGGVVVNVDVTLLAE
                     APRIAPHREAMRARVGQILGIGADRVSVKATTTEKLGFVGRKEGLAALAIATVRLP"
     gene            complement(17581..>18186)
                     /locus_tag="PK264_07575"
     CDS             complement(17581..>18186)
                     /locus_tag="PK264_07575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRD75780.1"
                     /translation="TFDEWDACVRAKGCSHNPGDQGWGRGKRPVINVSWQDAQEYVAW
                     LSKATGRTYRLLSEAEWEYAARAGTTTPFWWGSTISTGDANYDGNYTYAGGAKGVYRQ
                     KTEPVNSFKPNPWGLFNVHGNVWEWVQDCYADTYNGAPDDGVARENDPCSLRVLRGGS
                     WYFNPRRLRAALRSWFGPVDRDDYSGFRVARSLSASSARTD"
BASE COUNT         2948 a         6062 c         5677 g         3253 t
ORIGIN      
        1 cctcgatcgc gccgagcgcg cctgccgggc cgagcttgcc gagctcgacg atctcatcgc
       61 ccgcggcgcc tccgcccgcg atcagatcgc cgtccttgcc gagcaggggg cgtgcgctgc
      121 ggtgatggag aaggccgcgg cgagccttgc cgggatgagc agcgcaaagg gcaaggccaa
      181 gacaggtccc acgaccaagc cggccgctcc gggtgaattg aagcttctgg caaccttcga
      241 aggtcactcc aacgtcattc aagccattgc gttttcaccc gatggcannn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggacttg gagcgcaacc gccacattgc
      361 gacgttcgcc cccaactgcg gtaaggttca ggcgatcacc ttctcgcccg atggaaagct
      421 cattgcggcc ggatgctatg gaaatgtgag cttctacgat tggcaaagca agacgccacg
      481 aagcgatctt aaatcactga atggatatgt tttctcgctc gcatttcttg gcaacagcga
      541 gaaagctatc ggtatgatcc acggaggttc gtcatactcg atttttgtcg caccagcgcc
      601 cgcctcgccg ccactgatca gtcaaccgcc aaatgcagat cggaatatct tctacgttgg
      661 caatagcgta ctcggtgttg ctcacgctcc gaatggtcga atgactgcgg tagcttccag
      721 ggatggcgga accatgctct tcgatccagg taccgggaag cgcctgcggg cattttcgcg
      781 accgggccac gacgcctacg ccgttggttt cacgcccgac agcgaaattc tgattacggg
      841 agagtctgac tacaagatcg ttgtccggga catcggctcg ggtcagatca agagcctact
      901 caccggccac aaagtgcgcc ccgtcggtct cgccgtttcg tccaatggct cgcgactggc
      961 gagcggagat catgatggtc atctcatggt ctgggatctc cagtccggcg cgaccacgcc
     1021 gttggcgtcg gtgaaggcgc acggcaagag cgtgttcgcg gtcgcgatct caccgaaatc
     1081 acgcatcatt gcgacgagtg gagcggagga atatcaatcg ggtaaagggc atgtcggcca
     1141 ctcgatcaag ctctgggcga tgcccggccc ttgaggcggt cctgcaccac tgccgcgcga
     1201 ccgcccccac ctgtgctatc ctccccgcct cactcactga gaggccgatc cgtcccccat
     1261 gtcgctcgaa atcacgctgt cgcttgccgc catcttcctc ctgctgatgc tctccgcctt
     1321 cttcaacggc agcgagacgg cgctcaccgc cgcttcgcgg gcgcgcatgc acacgctcga
     1381 gcaggagggg aaccggaaag ccgcgctcat cagccgcctg ctcgccaacc ccgagcgcat
     1441 gatcggcgcg gtcctcctcg gcaacacgct cgtcgacgta ctcgccgcgg cactcgcctc
     1501 gaatctcgcg acccaccttg tcggcgacat cggcgtcgcc tatgcgaccg cgatcatgac
     1561 cgtgctcatc gtgatcttcg ccgccgtgct gccgaagacg tacgcgctcg cctactccga
     1621 caggatcgcg ctcgccgtcg cgcccgtcat gcggtgggtg atcaccatac tcacgcctct
     1681 gaccgccgcg atccagttcg tcgtccgact attcctcaag ctcaccccga cgaagaagga
     1741 cgacgacgcc aacattctcg cgacgcacga ggaactgcgn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc
     1921 cgcagaagat catcgaggac gcgctgaaga gccagtattc gcgcctgccg gtctggaagg
     1981 aggagccgga caacatcgtc ggcgtgctgc acacgaagga cctgctcgcg gccctcgccc
     2041 gtgccggcta cgaccctgcg aaaatcgaca tcatgagctt tgcggccgac ccctggttca
     2101 ttcccgatgg gacgagcgtc aaggaccagc tcaaccagtt cctgaagcgc aaggcccagc
     2161 ttgccctcgt cgtcgacgag tacggcgagg tgcagggcct gatcacgctc gaggacatcc
     2221 tcgaggagat cgtcggccag atcagcgacg agcacgacac cggcgacgtc tcgctccgac
     2281 agcagcccga cggctccgtg atcgccgacg gcggcctcgc catccgggat ctcaaccggc
     2341 agatggattg ggacctcccg gacgaggagg cgacgacgct tgcgggcctc gtcatccacg
     2401 aggcgcagac gatccctgaa tccggccaga ctttcacgtt ccacggcttc cgcttcgaga
     2461 tcatcaggaa atcgcgcaac cgcatcacgg cgatgcgcgt gcggcccgtg aaggccatgc
     2521 cgcagcgctc gcggccgacg gcggagatcg acccgccgta cagcggctga ggtcatgatg
     2581 gagacgagcg ccttccccta tcgccgctcg gcggaccagg atcggccgac gccggcgcgc
     2641 cacgatgtcg tgatcgtcgg tgcggggccg gtcggccttg ttctcgcaat cgaccttgcc
     2701 cagcgcgggt ataggccgat cgttctcgac cgcaagcatg gtctctcgga cgggtcgcgc
     2761 gcgatctgct ggtcgaagcg cacgctcgag atcatgggcc ggctcggcct tgccaaccgg
     2821 ctcgtcgccc gtggtgtcac ctggaagacg gggaaggtgt tcgcgggcga gcggcagctc
     2881 tacgagttcg acctcctgcc tgagcccggg catcgccagc cggcattcat caacctgcag
     2941 cagttcctgt tcgaggcgga ttgctttgcc gaggcggagc ggctcggcat cgaggtccgc
     3001 tcgggccacg aggtcacccg tgttgcagca gccgccgagc acgcccgcat tgagatcgcg
     3061 acgccggacg ggccctacac gctcgaggcg cgctatctgg tcgccgctga cggcgcccgc
     3121 tccaccgtcc gccactcgct cgggctcggc ttcgagggtc aggtcttcaa cgaccagttc
     3181 ctgatctgtg atgtgaaact cgagcttgac cggccgaccg agcgctggtt ttggttcgat
     3241 ccgccattca atcgcggcca gtcggcgctg ctgcatcgcc aggccgacaa tgtctggcga
     3301 ctcgacttcc agatcggccc agaggcggac cgcgacgagg agatgcggcc cgagaatgtc
     3361 gcgcgtcgcg tgcgcgccat gctcggcgac acgccgttct cgttcgagtg gatcagctgc
     3421 taccgcttcc agtgccggcg tctcgcacga ttccggcatg ggcccatcct gttcgtcggc
     3481 gatgcggcgc accaggtctc gccgttcggc gcgcgcgggg gcaactcggg cgtccaggat
     3541 gccgacaacc tcgcctggaa gctcgacctc gtgctctgcg gcgtgagtcc cgacgcactc
     3601 ctcgatacct atgacagcga gcgccgccag gcggcagacg agaacatcgc cgcctcgacg
     3661 cgctcgaccg atttcatctc gccgaaggga ccgggcgcca cgctgctgcg cgacagcgtg
     3721 ctcgcgctcg ccgagcggga acccttcgcg aggcggctcc tgaactcggg ccgcctttcc
     3781 acaccgacaa cctacgacgg ctcgccgctg aactcgcccn nnnnnnnnnt gggctggcga
     3841 atggcccggc ggcgctccgc ccgggcgcgc ccgcgctcga tgcaccactc ccccgaaacg
     3901 aaggcggcgg ctacctgctc gatcgcctcg acaagttcgt gctgctgagc gtcggcgaga
     3961 gcgtggcggc gggagcggcc ccgtccagca tgccgatacc cggcgtcgaa cagctgacgc
     4021 tcgcttcacc cgacgtcgtc cggcgctacg gcgccggggc aggacaggcg cattacctca
     4081 tccgacccga ccagcacatc gccgcgcgct ggagccatgc agcgccactt gacctcacca
     4141 ccacctgtga gagggcacgg ggccgcagca tctgatatcc atcgccctgc ctgacaagtc
     4201 tggagccccc atgaagaccc gcgccgccgt catccgccgg atggagcagc cccgccccta
     4261 tacggtgtcg aggccactcg ccattgagga actcgacctc gatccgccgg gcgagggaga
     4321 ggtgctgctg cgcatccggg ctgccggact atgccattcc gatctctcga ccatcaacgg
     4381 cgaccggccg cgccagatgc cgatggcgct cggccacgag gccgcgggcg aggtgatgga
     4441 gctcgggccc ggggtcaggg acctcgccaa gggcgatcac gtcatcctcg tcttcgtgcc
     4501 gagctgcggc cactgcaatc cgtgcgccga aggccgcccc gctctctgcg agccgggctt
     4561 caagtcgaac ggcgccggca cgctcgtctc cggcgcgacg cgactgagcc tgaagggcga
     4621 gcgcatctgg catcacctcg gctgctcggc ctttgccgag tacgcggtcg tctcgcggca
     4681 gagtgtcgtg aagatcgaca ggtctctccc cttcgaggaa gcagcgctct tcggctgcgc
     4741 cgtcatcacg ggcgccggcg ccgtcatcaa taccgggcgc cttcaggcgg gcgagacggc
     4801 cgccgtgatc ggcctcggcg gcgtcggcct gatggcgatg ctggcggcca aggtcgccgg
     4861 cgcccgccag atcatcgccg tcgacatgct cgacgacaag ctcaagcttg caaagcagct
     4921 cggcgcgacc cacacggtca atgcgcgcga tccgaaagcc atcgaggcga tccgcgacct
     4981 ctccggaggc ggtgtcgact tcggcttcga gatggcaggc tcggccaagg cgctcgagct
     5041 cgcctacaag gtgacgcggc gcggcggcac gacgatcacg gcgagcctcc cccatccgac
     5101 acacactatg gcgctgccgg tgacgaatct cgtcgccgag gagcggaccg tgaaggggag
     5161 ctacgtcggc agttgcgtgc cgagccgcga catcccgcgc ttcatcgcgc tctaccagca
     5221 gggactgctt cccgtcgatc gcctgatgag tgagcgcatc gcgctcgacg acatcaatat
     5281 gggcttcgag cggctcgccg aaggggccac cgtccggcag ctgctcattc cgtgagaatg
     5341 agcaaagtgc tggaaatcca gccctaaaca ccaatagcct ctatgcgggt tcgctcaaac
     5401 ccctctatga ctgagggggc gagggagcga ggcgcattgg ctgcaaccat caacatggcc
     5461 gcctacagcg atgcgctcgt cgtgctcggc acggcgggcg tggtcgtgcc gctggtcaag
     5521 cgcttcggca tcagcccggt gctggcctac ctcggagccg gcgcgctgct cgggccgctc
     5581 gcgctcggct cattcatcgg ctcgttcccg atcctctact ggttcacggt ggtcgacgcg
     5641 cagaacgtcg ccggcatcgc cgacctcggc gtcgtgttcc tgctgttcct catcggcctg
     5701 gagctctcct atgagcggct ctatgccatg cgccgcctgg tgttcgggct cggcgggttg
     5761 caggtgctgg cgacgacggt ggtgctttcg gccgtcattg cgctggccgg tgcgaagggg
     5821 acggcggcct tcatcctcgg atcgtgcctc gcgctctcct cgaccgcgat cgtcctcgag
     5881 gtgctctcga gccagggccg gatgccgacg agtgccggcc gcagcagttt tgccgtgctg
     5941 ctcgcgcagg acctcgccgt catcccgatc ctgatgttcg tctcgatcgt cggcagcaat
     6001 ggcggcagct cggtgctcgg cacgatcggg gccacaatct atcacgccgt gattgccgtc
     6061 gccgtgattg tgctggtcgg gcgcacaatg ctgcgcccgc tgttccggat cgtcgtcgcg
     6121 acgcagtcgt ccgagctgtt cattgcgacg atcctgttcg tgatcgtggc gaccggcgtc
     6181 gttgccgccg ccgccgagct gtcgatggcg ctcggcgcgt tcgtcgccgg cctcctgctc
     6241 gcggagaccg agtacaggaa ggccatcgag gcgacgatag agcccttcaa aggcctgctg
     6301 ctcggcatct tcttcttcac cgtcggcatg agtatcgacc cgcgcgagct tgcccgcgag
     6361 ccgctcctgc tcttcttgtc ggtgatcggc ctgatcggcc tcaaggccgc aatcgtcctt
     6421 ggcctcgccc ggctcttccg cttgacctgg ccgacctcga tcgagacggc tctcctgctc
     6481 ggacctggcg gcgagttcgc attcgtcggg atcgggctcg ctgccacact cggcgtcgtc
     6541 gcgaagtcga cggcgagctt tgcgcttgcc gtcactgccc tgacgatggt gctcattccg
     6601 gcgctctcct atctcggcca gcgcttcggg cggcgcatgg aaccgccgaa gcctctcgat
     6661 gccgccctac tcgcggtccc ggagcagaag gaccatcacg ccatcgtcgt cggttatggc
     6721 cgcgtcgggc gcgtcgtgtc ggaactcctt gaagaacaca gcatcgccta cacggccgtc
     6781 gatcatgacc ccgtcagcat cactcgcgcc aaggcagatg ggcatcccgt ctactacggc
     6841 gatgccacca gcgaggcgtt cctaaaggca tgcggcctca tggaatgctc gggcgtgatc
     6901 atcacgatcc acgatcaggc catgctcgac gagattgtgc tgctcatccg ccggcttcgg
     6961 cctgacatca tgatcgtcgc acgggcgaaa gacgccgtgc acgcaggcca tctctacgcg
     7021 atcggcgtca cggatgccgt ccccgagacg atcgaggcga gcctgcagct ctccgaagcg
     7081 gcgctcgtcg gcctcggcag gcaggcgggc ccggtcatcg cctcgatcca cgagcatcga
     7141 gacagggtgc gccaggtcct ccagcaggcc gccagtaagg ctggcgcccc gcaatcacgg
     7201 gcgctgcgcc agaagctccc cggaaaactc tgaggcctcg cctcagccgt cgagatccat
     7261 ctcgaggatg gccatctgca gctgccaatc gcggctgccg tcctccgtgt cgagtgttac
     7321 ggtcgcgagg aactcatcgc cgatgtagac ttcggccgaa tcagtcttct tcgggcgtgc
     7381 gcgcacctcg aggttcggcg tgccgaaggt cttgcgcaga taggcttgca gcttggcgag
     7441 gtcggtcttg gtgatccctg gcgcctgccc cggtttgctc tggcctgcca tgttgcatgt
     7501 cccttggtga tggaggcgcc gccgcgccaa gtattccgcc gagcggatag cacaggacgt
     7561 cgcgtgctgt cgcctgtcac atctcgccga ggttgagaga ccaccatgac cgacgacgcg
     7621 ctcctcagtg ccttcctgga gatgatggcg gccgagcgcg gcgccgcccg caataccgtc
     7681 gaagcctata ggcgcgatct cgaggatttc acggcggcgt tgcatcgatc ggggcacacg
     7741 atcgtcacgg ccgactgtgc ccgcatcaac ggctatctgc gtgatgcaac cgccgccggc
     7801 ctttcagcgg cctcgcgggc gcggcgcctc tcggccatcc gccagctcta tcgcttcnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaaggagc
     7921 gggcgctgcc tcggaccctc tctgtcgccg aggtcgaccg gctgctggac accgcacgca
     7981 atcgcaccgg ggcggccgtc ggcatcgagc gtatacgggc gctgcgtttc agggccctca
     8041 tcgagatgct ctacgcgacg gggatgcgcg tctccgagct cgtctcgctc ccgcgcaacg
     8101 tgctcgacgg cgatggacgc atgctcgtcg tgaaaggcaa gggcgggcgc gagcgcctcg
     8161 ttccgctcaa ccagacggcg cgggcggcac tcgacgattt cctcgccatc ggtaccggcg
     8221 gcgacgacct cgcccgcatg gtgcgcacgc gctggctctt cccgtcgaag tcggcggagg
     8281 ggcatctgac ccgccagcgg gtcggccagg acctgaaggc cctcgctttc gaagcggggc
     8341 tcgatcccga gcgcgtgtcg ccgcacgtcc tccgccacgc cttcgcgagc cacctcctcg
     8401 accgcggcgc ggatttgcgc tcggtgcagc agctgctcgg ccacgccgac atctcgacga
     8461 ccgagatcta cacgcacgtg ctcgaagagc ggttgaaggc tctggtcgag cgccatcatc
     8521 cgctggcagg tccgcgctca tgagcggaga ttgagagctt ggccgaggcc agggctgtgg
     8581 atgcacggtc ggcagctgga gagagatgac gctgagggag ggagtgggtc gctggcggtt
     8641 ttgacctgaa agagacaagg ggcgcaagcg tcgcctcacc tccagtgtca tggtcgggct
     8701 tgtcccgacc acccatcatg ccgcaacctc cgcccgtcga gatgggccat aagggtgcat
     8761 cccccgtccg cttagggtag gcaagtttgc gtccgctcct gaagtaactg ccgactcgct
     8821 ctgagccaga accttatttt ccgagtgccg ccttgatcct ggcggttatc gccgtggtcg
     8881 agattccgta acgatcgtgc aacgtcggca gcgctccggc atcgaggaat gcgtcgggca
     8941 ggccgatgtg ctcgaacgct ggatggactt tcgctcgcat cagcgtcgcc gccaccgctt
     9001 ccccgagacc gcctatgacg ctgtgatttt cggccacgac gacgagacgg ccgggcttct
     9061 tgcaggcttc gacaatcatt gccgtgtcga gcggcttgat cgtcggcacg tgcagcacgg
     9121 cggcgttgat cttgtccttg gcaagttctt ttgacgcttc gagagcccgc atggtcagca
     9181 ggcccgacga gatgatcaga acttcggcgc catcacagat cagcttcgct ttgccgagtt
     9241 cgaacgtgta gccgtactca tcaagcacga gtggcacctg cccgcgcagt aggcgcatat
     9301 agactgggcc cttgtgggct gcgatcgcat gggttgcctg ttcagtttcg agcgcatcac
     9361 atgggtcgat taccgtcata ttcggcatcg cgcgcatcag cgcaacgtcc tcagccgcct
     9421 gatggctcgg gccgtagccc gacgtcagcc cgggaagcgc gcagacgatc ttcacgttgc
     9481 gatcttcctc ggcgattgtc tggtgtatga aatcgtaggc acgccgtgtg gcaaagacgg
     9541 cataggtcgt cgcaaacggc gtgaatcctt cggcggccat gccggatgcg gcgcccaaca
     9601 gcaactgctc ggccatgccc atctggtaga agcgatctgg gaacgcctcc ttgaagatgt
     9661 gcaagtcggt gtacttgcca aggtcagcgg tcatgccgac aattttcgga tcagccttgg
     9721 ctgcggccac gagtgcatgc ccgaacggcg ccggcttcgt cttctgtcct tcgcctgcaa
     9781 tcgaggcgat catcgcagac gtggtcaggc gcggtttgtt gggatcgcta gttggagctg
     9841 gacgcttcat gcatgcctcc catcgtcgag ggctttgagg gccaactgcc attcgtgggc
     9901 gtcgacgcgg atgaaatggt tcttgtctcg ggcttcgagg aagggcacgc ccttgcccat
     9961 cagtgtgtcg gcaatgatga tgctgggacg gccaccgttg ttcgacttag cggcatcgaa
    10021 tgccttcacc acggcatcga ggtcgttgcc gtcgacccgc tgcgtgaacc agccgaacgc
    10081 ctgcatcttg tcgaccagcg gctcgaacga gagaactccg gtggatggac cgtcggcctg
    10141 ttggttgttg atatcgacca cggcgatgag gttatcgagc ttgtagtggg cggccgacat
    10201 gatggcttcc cacttcgagc cttcgtcgag ttcaccgtcg gagaacagcg tgtagacgcg
    10261 cgcatcggag ttcttgcgct tcaacccgag acacatgccg accgcgatgc tgaggccgag
    10321 gccgagcgat ccacctgaca tctccatgcc cggcgtgtac gaggccatgc ccgacatggg
    10381 caggcggctg tcgtcgctgc cgtaggtttc gagttcgctt tccgggacga tgccggcttc
    10441 gatcaaagcc gcgtagagcg caatggcgta gtgcccattc gacagcagga agcgatcgcg
    10501 gccttcccat gccggatcat cggcgcggta tttcatggcg tggaagtagg cgaccgccag
    10561 cacatcggcg atgtcgagcg cctgggcgat gtaaccttgc ccctgcacct cgcccatgcg
    10621 caccgcgttt cggcggatgt ggtaggcgcg atcggcgagt gtcttgttcg gaagcgcggt
    10681 cggcttagcc atgaatcagc atcccgccat tgacgtcgat gacggcgccc gtgatgtagg
    10741 acgacaagtc tgacgcgagg aacagaaagc aaccagccac gtccttcacc tcgccaagac
    10801 gattcagcgg aatgcccgcc gcgatttctg tcttcatctt cggtgtgagc ttatcgccgg
    10861 tgatgtcggt ctggattagg ccgggcgtga cgcagttgac gcggatgccg tcaggcccaa
    10921 gttcacgcgc catggccttg gcaagaccga ggatgccagc tttggccgcc gagtagtgcg
    10981 ggccgccgaa gatgccgccg ccgcgctggc cggagactga cgaagtacat gcgatggagc
    11041 cggacttgcg gcttcgcatg tgggggatgg tcgcctggga caggtagagg gtgccgcgca
    11101 ggttgacatc gaggatgcgg tcgtaactcg ccgcgtcgat ctccatgagc ttcaccggct
    11161 gggtgatacc cgcgttgttg atcaggatgt cgacgtggcc gaacgccttg acggcggcat
    11221 cgacggcagc tttgcactgg tcgagcttgg tcacgtcgca ggcaagaccg atgtggtcct
    11281 tgccgaggga cccggcagcc tcgatcgcgg ccttgccgtc gatgtcaagg atggcgacgc
    11341 gagcgccgtg gtctgcaaac agcttggcag tggcgaggcc gatcccgcgc ttgccggcgg
    11401 cacccgaaat gaccgcggtc ttacccttca gcagcatggt ggtttcctcc aagtcgcata
    11461 cgcacccttg ggcgcgttga tgagcgggcg tcagcctctt ggcaaacgca gagatttcac
    11521 cgcacagtga attcacttca cattttgcgc tgcagaaggc attgcgagag cgatcaatgt
    11581 gagtatcgtg atgaaggatc acgctagcca tgatcgggtc cgtctcactc aagggtatcc
    11641 gagcttttga ggccgtcgcc cgccttggct ctttccgcgc tgccgccacc gagatgaagc
    11701 tgacctccag cgccgtcagc cacgcggtca ttgacctgga aagggacctc ggcatcggtc
    11761 taattgaccg ccagaagcgg ggaggccgcc tcaccgagcg cggcgaggcg ttctatgcgc
    11821 atgtacgcat cgcctttgat caactccgcc tcggcctcga cgaagcctcg cagcggatgc
    11881 gtaggatgtt ccgcctccac gcggccccaa cattcgcggc cggctggttg tcgccgcgac
    11941 taccgcgctt tttggcgctt catcccgaga tcgaggtgcg actgtcggcg gggacagact
    12001 acagcagttt ctccaacaac gatttcgatg ccgacatcgt ttacggcccg gtgcgcgcga
    12061 ccaatgtgac gatcgtgccg atcatcgaag agacaatcgc accgatgtgc gcgccgtcga
    12121 tcgcggcatt gatcacaacg cctgccgacc tgcgccggca agtcctaatt cagagcgaga
    12181 cgaagatggt gcggtggtcc cattggttcg agaagaacgg cgaaccgtca cccaacatcc
    12241 atggcatacg tttcgaccgc agcttccttg ccattgccgc cgcagcagac ggcctcggcg
    12301 ttgcgcttga gtcgacgctg cttgcggagc gcgagcttgc gagcgggcgc ctcgttatgc
    12361 ctttagccgg tcgggccaag gacatcacgt acgttggcca cagcctcgtg tttccaaaac
    12421 tggcgaagcc gaacaagctg gtcggcgtgt tcgccgaatg gctcaagcag gagatcggtg
    12481 atagcaaaat gaccaccgcc gaatgatggc caacggttcc aagagcgcgc gaccatgtcc
    12541 gctgccggaa aatgctgacc tcaaaccgga cgaaggaaac aaaggcggtc attgtcattc
    12601 tcgacgtttg gagcttgccg cgtgatgggt ggtcggcaca aggccgacca tgacagcggc
    12661 gggtgtggcg atggtggtgc cacgtgtcgg ctcctggccc atctcgacag ttggagacta
    12721 tgccgcgatg ggtcctcggg accagtccgc gggcgacaat ggtgggtgtg gcaacggcag
    12781 cacctcacgt cttcgcctcc ctcccaccca tcacgcagag actggccgcc ttgtcgctgc
    12841 ggccatgagc tgacattgga tcgcgggaat tcgttggtca actcgacgaa gcctgcttcg
    12901 gcgcgtcgtc gcccggcgct cgacaaaggc cgggacagcg ccgcatcgcg cgtgacaggc
    12961 tcgtgaccgc gggcgataaa tcgggatcgg tctcaccacc tcccacgacg aaagcgctcg
    13021 cgtgccccgc ggaccctggc ttcccccagc gctgaccaag ccgcgctacc gcatctatgc
    13081 ggtcggcatg ggcatctcgc tgatgggcta ctgggtgcag acgatcgcgc tctcctggct
    13141 cgtctaccgg ctgacggatt cggtcttcat cctgggtctt gccggcttca tgcagcagat
    13201 cccgtcactc gtcgtgccgc cgatcgcggg catgctcgcc gaccggctgc cccgcgtgaa
    13261 gctcgtcctc atcctcaatc tcgtcctcgg cctcctggcg ctgacgctcg cgatcctcgt
    13321 ctggtcgggc gtcaaggacg tgcgcatcta tctcgccatg gccttcctca ccggctgcgt
    13381 cgccgcctcc gaggcgccga accggcagtc gctcgtcggc gccatcgtcg aggaaccggc
    13441 cttgctgccg agcgccatcg ccttccactc cgtcattttc aacaccggcc gcatggtcgg
    13501 cccgaccgtc gccgggctcc tgctgctcgt catgagcgag gcctggtgct tcttcatcaa
    13561 tgccatctcg tatgtcgcgg tgctcatcgc cttccgcatg atcggcttga aggacggcgc
    13621 gcgggcaaag agccaggaga gtttcatcgc catgctgcgc tcgacggccg cgcgcgtcgg
    13681 cgagttgcct gtcgcccgct atttcctgcc gctggtcgca acggccggcc tcctcgccgc
    13741 gccctatcag gcgctgatgc cctcctatgt cgacgacgtg ctcggtggcg gcacgggcac
    13801 gctcggcctc ctcgtcggtg cggccggcgc cggcgcactg tccgccgcga tctacctctc
    13861 cctgcagacg ggcgggcggg cgcagctccg cctcgtgcag ctcgcccctt tctcgctcgg
    13921 cctcgccgtg atcggcttcg gcctctcgcg ttcgctgccg ctctcctgcc tctgcctcta
    13981 tgcgatcggc gcctcgatca tgtgcacgac ggcgacgacc aacacgctcc tccagctctc
    14041 ggtcgatccg ggctggcgcg cgcgcgtcat cggcatcttc cagatgtcgt ttgcgggcgt
    14101 taatcccgtc ggcagcctca tcgccgggct cgtcgcggcg cgcctaggca tcggcccgac
    14161 cttcgtcctc aacggcgccc tgatcctcct cgtcgggttg ctgatgcgtt ggcggcttaa
    14221 gcgctcgccg gacgccgccc gtgagctcga gcgtgtcgtc ggcggctgac gagatttcat
    14281 tgcggctgca tatttctcat gcctgcctgc gaccgtcgct ctatggatta gcggctccaa
    14341 cgccgttatt gtgacggccg atcggcgagc ggacggcccg ccacagcttg gaacgagcac
    14401 gacagatgaa ccgtttcgaa cgcctgttcg gcctcagagg cgaggcccgc gtccgcttct
    14461 cggacggcga gtatcaggtg atcagccccg gcgacttcgt cacctgcgcc gtgaccggca
    14521 agccaatccc gctccccgac ctcaagtact ggaacgtcga cctgcaggag gcctatgcct
    14581 cctccgagat ctccttcaag cgcgcgctcg agctgcgcgc agcgcgccgt cgttgatccg
    14641 ggcgcgcaag atggcgcccc gcaagccgaa gatcgccgcc gccgagctcg atgatcgccg
    14701 cgaggtcggc cggctgcacg aactgcatcc gcgccaggtg ttcttggcga tgagcaccgc
    14761 cggcctctcg cccggtcgct ccggcaatgt ctccgcgcgc cacgccgatg gcatgctgat
    14821 cacgccctcg ggcgtcgcct acgatacgct cgatccggcc gacatcgtcc acgtgcgggc
    14881 agatggccgc gtcgatcccg gccagctgaa gccctcgagc gagtggcgct tccacctcgc
    14941 gacctacgcg gcccgccccg atgcctgtgg catcgtgcat tgccactcgc tgtcggcgac
    15001 cgcactcgcc tgcacgaaga agcccatccc ggcctttcac tacatggtcg cggcggccgg
    15061 cggcgccgac atccccgttg tcccctacgc gaccttcggt accgaggagc tgtcgcgcca
    15121 cgtcgtcgcc ggcctcaagg atcgcaatgc ctgcctgatg gcgaaccacg gccagatcgc
    15181 ggtcgcggcg agcgtcggcg cggcgctcga gctcgcccac gaggtcgagg tgctggctga
    15241 gatgtacatc aaggtcttgg caatcggcgc gccgcacatt ctcgatgcgg cggagatggc
    15301 gcgcatgctg acgctcttca ggtcatacgg ccagcgcgcc caggacggtg ccaaaccaga
    15361 gggcaaggca aaggcctgag ttgatcatgg cagccgcacg gtcgcgatcg cgagtgcggc
    15421 gagcccttcc ttgcggccga cgaagccaag cttttccgtc gtcgtcgcct tgacgctgac
    15481 gcggtcggct ccgatgccga ggatctggcc gacccgcgcc cgcatggcct cgcgatgagg
    15541 cgcaatgcgc ggcgcttctg caagcagcgt cacgtcgacg ttgacgacca cgccgccgcg
    15601 ctcacggacg cgccgcgcgg catcagcgag gaactggtcg gaggcggccc cgcgccagcg
    15661 ctcgtccgac ggcgggaagt gctggccgat gtcaccgtcg ccgattgctc cgagcagcgc
    15721 gtccgtcagc gcgtgcaggc cgacgtcggc atccgagtgg ccttcgagtg cgcatgcatg
    15781 cgggatgcgc acgccgcaga gccagacatg atcgcctggc ttgaaggcgt gcacgtcgaa
    15841 gccctggccg atgcgcacct cgccttctcg cgagagcatg tcgtcggcca ttgcgagatc
    15901 ctccgcggtg gtgagctttc ggttcgcctc gctgccctcg acgatgccga cgggaagccc
    15961 cgcccactcg gcgagtgcgg catcgtcggt gaagtcgtgg cggccggcgg tggcggcctt
    16021 gcgatgcgcg acgagtatct cggcaaagcg aaacgcctgc ggggtctgcg ctcgccagag
    16081 cttgtcgcgc ggctgcgtcc ccgtgatgcg tccgatggcg tcgacgatct tcaaggtatc
    16141 cgccacggcc aaacccggaa tgacggccgg atggcgatcg aggccgccga tgatgcgatc
    16201 gagcagcgcg cgcgtcacga atggccgcgc ggcatcgtgg atgagcacgg tttccggcgg
    16261 cagaactgac gcggcgagcg cctcgagccc ggcgaggccc gaggcttgcc gtgtcgctcc
    16321 accagtcacc ggcgccatga gccggctgtc gcgatcgagc ccagccagta tggccgcgac
    16381 gcgtgccgca tccacccgcg ggcagacgac agccacctgc tcgacccgcg gctcggcgag
    16441 gaacgcggca accgtccgcc ggagcagcgg cacgccgccg atcggctgat actgcttcgg
    16501 aaccggcccc gcacgcgtcc cctgcccgcc ggcgagaatg atggctgcta tggtcaacgg
    16561 ataggctctc gctgtttcgg gaccggccag cgttctgcaa tgacgcgccg cccgatgcaa
    16621 tggccgtgcg tgaccgctgc taccccacag cagagtgttc acgcctccgc tctggttctc
    16681 cggacgagcg ttcacgcgct ctcaggagag ccgatgctca gggcgtaggt tggcgctgag
    16741 cgcagcgaag cccaacattc ccggatgcga gcggaagcgt tgggcttcgc gagctcagcc
    16801 caacctacat ctggctcttc ggcagctttt cgagcgcggg cacgagccag atcaagaacc
    16861 tgtagtgcgc ctccagcgcc ggtccggttc gacgagcccg gtccttcatt cgcgtgagcc
    16921 ccaggcgagc gccgcggcgc cggtcggagc acgtatgtct cccaggacct gcgccgcggt
    16981 ggtttcgagc gacccacctc gcctctcgtt ggcgccgttg cagagcatgc gtcggcgcat
    17041 ggggtctccg gttggttcag gccctcgacc gtgctcatca tgggccgccc tggacgctcg
    17101 ctcagcactc ccggcaagac cgtgatcttt ccggctctgg ctgcccgcaa tcgggttcgt
    17161 cgagcgtgct cgcggaagaa gccgaaaggg aacgagccac ccggaagcct gaattgttgt
    17221 tccggttgtc gggcctgttc ctgttgcggt tggccgcacg gagcctcctg gggttgttgt
    17281 tccaggagcc accccgcagg acgcggtttt agggctgccc aggctccggg ataaacaaac
    17341 tcgacctctc ccgaagaatt gagggccagc attccaccag ccctcagcct cacaattacc
    17401 cgctcaagac acaggtgtcg acctcaaagc aggcgatttc cacgagcaaa ttgcggccaa
    17461 ttcgcgatcg atgggactaa aaaaaaatcg agcgcttcgc gcttggacga ttcccaatcg
    17521 aacgcgaatc gagtataaac ttcaatagaa ttaaggatgt aaagagtaag aatgcaggag
    17581 ctaatcagtc ctcgcggaag aagccgaaag ggaacgagcc acccggaagc ccgaatagtc
    17641 gtcccggtcg acgggcccga accagctgcg gagggccgca cggagcctcc tggggttgaa
    17701 gtaccatgag ccaccccgca ggacgcggag tgaacatgga tcattttcac gcgccacgcc
    17761 atcgtccggc gcgccattgt aggtgtccgc gtagcaatcc tgcacccact cccacacgtt
    17821 accgtgtacg ttgaacagcc cccaaggatt gggcttgaag ctgttcaccg gttctgtttt
    17881 ctggcgatag acgcctttag cgccaccggc gtacgtgtag ttgccgtcat agttcgcgtc
    17941 gccggtcgag atcgtgctgc cccaccagaa cggcgtcgtc gttccggcgc gcgccgcata
    18001 ctcccactcc gcctcgctca gcagccggta tgtccgcccc gtcgccttgg agagccacgc
    18061 cacgtactcc tgcgcgtcct gccacgatac gttgatcacc ggacgcttgc cgcggcccca
    18121 gccctggtca cccgggttgt gcgagcagcc cttggcgcgc acgcacgcgt cccactcgtc
    18181 gaacgt
//
LOCUS       DAORNH010000053        18057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2375_length_18057_cov_2.803633, whole genome shotgun sequence.
ACCESSION   DAORNH010000053 DAORNH010000000
VERSION     DAORNH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_18057_cov_2.803633"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>366)
                     /locus_tag="PK264_07580"
     CDS             complement(<1..>366)
                     /locus_tag="PK264_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131781.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRD75781.1"
                     /translation="IRMTWHSAGTYRTADGRGGGGTGNQRFAPVNSWPDNGNLDKARR
                     LLWPIKQKYGRNISWADLMVLAGNVAMESMGFKTFGFAGGRADDWEPELVYWGPEAEM
                     LGRKRYDEKGKLENPLAAT"
     assembly_gap    367..435
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<436..635)
                     /locus_tag="PK264_07585"
     CDS             complement(<436..635)
                     /locus_tag="PK264_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75782.1"
                     /translation="MTNEAKCPVMHGTNPATRSSARSNRDWWPNQLNLKILHQHAPAS
                     NPMSAGFNYAAEFKKIDYAGLKR"
     gene            complement(715..>948)
                     /locus_tag="PK264_07590"
     CDS             complement(715..>948)
                     /locus_tag="PK264_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75783.1"
                     /translation="GGMSTLLAWLDKLPWWLVIGGCLTLGLLPYIPEPHIWEKLRLLA
                     TGTLTRPIDIFDLIYHALPWLLVVAKLARRLWP"
     assembly_gap    949..961
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1302..1859)
                     /locus_tag="PK264_07595"
     CDS             complement(1302..1859)
                     /locus_tag="PK264_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75784.1"
                     /translation="MAAYHVVLVHFPIALWMTATLAILWRAVSRGPVALAIDRALVPL
                     LTVGLISGLLAITVGRFAWSWEAISTSPMGRNHMLLAVWTTAYWALMLVTRVVQGEAI
                     WDGPGRWIMAALALLGSGLLGVTGTLGGHLSGIYTDLAKVLRMLGWEVHRTFYVPNMT
                     IGIIAAAALALLVIASLGSSGKKSS"
     gene            complement(1862..3292)
                     /locus_tag="PK264_07600"
     CDS             complement(1862..3292)
                     /locus_tag="PK264_07600"
                     /inference="COORDINATES: protein motif:HMM:NF021010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HRD75785.1"
                     /translation="MSQARTPWLLTSLALFLGTLAVSWLTHGKGVVTSDPASHRSVPA
                     ALTVPLSVQAAYNGRDIVFRYRWPSPNPGIFHDMLRFEGGKWVVKGSAVPGSQPDGLH
                     EDRVAMMLDDGSVPEFSRYGAYITVGHRLAGLTNDVSGKDVAKHPYLGAKLKQDELTK
                     YLPATRSKIDDWASVVPEDELNALRNAGYFLDLWHWRANRSNPVGLADDQLVAEARLS
                     DAGKGMYVTNWDGEKKQPRLMFDAGKAGHKALKWDDLKVGKITQDVASALREDIAMPF
                     DPGAGWQDGDTLPRRIVRPGDGSRADIKVVGKAAWKDGFWDVTLTRKMDTGNKREDKI
                     LVDRGIYHVAFAVHRQATGGRWHYVSLPFSLGLGRAAEITAARFEGDQPKWDQPAKTI
                     TLFYPGQVTWGHVNSAAHAGAEKVRAGVPVRHRHSEAELTHYGIEMEYDAEIKRQWLL
                     TMLAGVLLIAAFGIALNRTLGPGKGV"
     gene            complement(3470..4675)
                     /locus_tag="PK264_07605"
     CDS             complement(3470..4675)
                     /locus_tag="PK264_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRD75786.1"
                     /translation="MKVTAIETIRSEEFPNILWVEVMTNEGIVGLGETFYGPDAAEGH
                     IHGIIAPYLLGKDPRNIEAHQAFLTGYVGFVGSSAEMRGRSAIDIALWDILGQSVKLP
                     LCDLLGGRVREKIRVYNTCAGYSYVQTKVTQGTANFGLDARKGQYEDLDAFLNRAGEL
                     AESLLEMGISAMKIWPFDYAAERSSGYDITAEELKSGLAPFEKIRKAVGDKMEIAAEL
                     HSLWSRPTAVKIARALEPIRCMWVEDPVFMDHLHSIGEVARATRSPIAVGETRGGRAD
                     FRGLLELEALSMVILDLSWCGGISEARKVATMAETYHVPVAFHDCTGPVVLAVSTHLA
                     LNSRNCWTQEMVRAFYYGWYHRFVTELPPVERGMITVPKGPGLGLALQPGVKTGKGMR
                     IRRSDAKGM"
     gene            4936..5310
                     /locus_tag="PK264_07610"
     CDS             4936..5310
                     /locus_tag="PK264_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75787.1"
                     /translation="MLKSMMAALVAAGAVTIAVAADASAHERRASRTTDSGAVLTEHP
                     KAGTYRRGPRVKGFVARRGGYSYSWFDSINTYGSSRTVYGGTFLYRDPMMDRQTGAGP
                     FDHGFFFDSGVGPRGGDSPYMH"
     gene            complement(5336..6379)
                     /gene="obgE"
                     /locus_tag="PK264_07615"
     CDS             complement(5336..6379)
                     /gene="obgE"
                     /locus_tag="PK264_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRD75788.1"
                     /translation="MKFLDQAKIYIRSGDGGNGCVGFRREKFIEFGGPDGGDGGRGGD
                     VWAECVDNLNTLIDYRYQQHFKAGNGKPGMGRNRSGADGGDVTLKLPPGTQIFAEDNE
                     TLLADLKVPGETALIAKGGNGGFGNAHFKSATNRAPRRANPGLPGEELTIWLRLKIIA
                     DAGLVGLPNAGKSTFLAKVSAAKPKIADYPFTTLHPNLGMVRIGGLDFVLADLPGLIE
                     GAHEGVGLGTRFLGHIERCRVVLHLVDATSENVVEDYRVVRRELAAYGAGLDKKKELV
                     ALTKCDALDEETIAEREEALKRAARKQPLLISAVSGAGIDEVLYALAREIKRSTTKEK
                     AAEGAEKPAPWRP"
     gene            6661..8712
                     /locus_tag="PK264_07620"
     CDS             6661..8712
                     /locus_tag="PK264_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRD75789.1"
                     /translation="MPIVQISQSSILNGVTSGDQTDPQTIRWEGGSVATYTSVEGGVA
                     VIRLATFNHEGERIGSDIVVSQVPSTDNMSTAFLGRLSNGTIIVTWVDVTTGEAFFRA
                     YDGTTLAPLNAPTEITQTSAVDATFSGIGFAEAANGNVLFSWTTTEASGDGAGLAVHA
                     RMLSADLSTFESEFQLNSTTTGDQARARVSQLGNGNFLVTFQTNDSGTDDIRGRIIDQ
                     NGDSVGNDFVINGTTAGDQQRARARQLDDGRTLIVHESNDGGSDVNIRMRLLDLDGVP
                     IGADEIVNTTTTGNQRAPNLIVLPDGRVAISWSSFEVSGSGGTQVVMRARIWDPDGLF
                     PESDFNLGQFPTTNSIPFTSLRATEDGRLVLAVGTPSLSLDGSGLSTELRYFVPTTDL
                     DEEVANGTTGPDPALIGTSLTTSWIDGLAGDDSIEMAGTGRAHFYGDAGNDTLIGSWN
                     TDTLYGGTGNDQLSGAANADILAGETGNDMLDGGAGGDVLDGGDGFDFVSYLSATSGL
                     TVNLSSPDLNTGDAFLDAYEGIEGLVGSSGNDNLTGTSGNDVIYSALGNDIMYGLQGN
                     DTLLGQAGSDIFFGGEGNDFMSGVNGAEVDVNIFVFNVSQYYGFGQDTIVGFDANDAS
                     PGHDVLYISSAVFADFAAMEASTFQFGTATVIFRPGSSDWIKLVGVDKTTLTAGDVVF
                     F"
     gene            8821..9630
                     /gene="dapB"
                     /locus_tag="PK264_07625"
     CDS             8821..9630
                     /gene="dapB"
                     /locus_tag="PK264_07625"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRD75790.1"
                     /translation="MSNKMRLAILGGAGRMGRELARAIAASDGAVVAAAIEPKGSASI
                     GRDAGDLAGLGASGVEITDDLAQVVGAIDGVLDFTIPKATCAAAAITAKAGLVHVIGT
                     TGLSAAEEAEIATAAMRSVIVKAGNMSLGVNLLTAITRKVAAALDPDFDIEVLEMHHR
                     HKIDAPSGTALMLGQAAAEGRNVTLTSVSVRSRDGHTGARRRGDIGFATLRGGSVVGD
                     HTVIFAGEGERIEITHKAADRGIFARGAVKAALWARGRRPGLYTMTDVLGL"
     gene            9634..>9964
                     /locus_tag="PK264_07630"
     CDS             9634..>9964
                     /locus_tag="PK264_07630"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD75791.1"
                     /translation="MQVAVETQIIAPPETVFDTLADIQRWPEFISAIEAIELLNADTF
                     AQGLRFRETRIMFGRKASEEMTVVTLERPRLMVFTAESHGTRYRSEHRIAPEGTGTHL
                     TLMFEGRP"
     assembly_gap    9965..9974
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10139..10963
                     /locus_tag="PK264_07635"
     CDS             10139..10963
                     /locus_tag="PK264_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRD75792.1"
                     /translation="MTAHQTAKAQAFRALHGRGSAFIIPNPWDVSSARILTGLGFEAL
                     ATSSGAAAGTFGRRDGGITRAQSLANARAIVEATDLPVAADLENGFGHGADEVALTIR
                     EASGIGLVGGSIEDCTGGKDGGIYPIDVAVARIKAAAAAVGALPVPFVLTARAENFIR
                     GRPDLDDTIARLKAYEAAGAEVLFAPGLPDITAVKRVCSALTKPFNFMVGIKGKSFSF
                     AELESAGVVRVSLATSLYRAAMTGLIEAAREARDKGTFGFVDRSLPTPDINAALKA"
     gene            11209..11547
                     /locus_tag="PK264_07640"
     CDS             11209..11547
                     /locus_tag="PK264_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRD75793.1"
                     /translation="MKKIEAIIKPFKLDDVKEALQEIGLQGITVIEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEIVLADEMLEKALEAIQRAAKTGRIGDGKIFISTIEEAIRI
                     RTGETGAEAI"
     gene            11694..13103
                     /gene="glnA"
                     /locus_tag="PK264_07645"
     CDS             11694..13103
                     /gene="glnA"
                     /locus_tag="PK264_07645"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRD75794.1"
                     /translation="MKTPADVLKLIKEKDVKFVDLRFTDTKGKLQHVTADVSCVDEAM
                     FAEGYAFDGSSIAGWKGIEASDMTLMPDASSVHLDPFFAQTTLAVFCDVIEPSTGEPY
                     ERDPRSIAKRAEAYLQQTGIGDRVYVGPEAEFFIFDDVKFSADPYNTGFKLDSSELPS
                     NSDTDYEMGNLGHRPRTKGGYFPVPPVDSCQDIRSEMLSVMAEMGVVVEKHHHEVASA
                     QHELGIKFGPMVTMADHMQIYKYVIHNVAQAYGKSACFMPKPIFGDNGSGMHVHQSIW
                     KGSTPTFAGNKYADLSDTCLHYIAGILKHAKAINAFTNPTTNSYKRLVPGYEAPVLLA
                     YSARNRSASCRIPHVTNPKAKRVEVRFPDPAANPYLGFAAMLMAGIDGIQNKLNPGEA
                     MDKNLYDLPPAELEKIPTVCGSLREALNSLDKGRDFLKKGGVFTDDMIDAYIELKMAE
                     VERFEMTPHPVEFDMYYSV"
     gene            13310..13705
                     /locus_tag="PK264_07650"
     CDS             13310..13705
                     /locus_tag="PK264_07650"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD75795.1"
                     /translation="MAWTHHELDRMSEAGLFAPGERVELIDGEIIPIPAKGQRHDRVR
                     MALNRSISRRLGEDKDLLPEPGWRPAERFYFEPDFLIVPRSVIVPEFARTEVLLGIEI
                     ADSSICYDTTIKRDRYAGLGVVEYWVVNA"
     assembly_gap    13849..13893
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14009..14719
                     /locus_tag="PK264_07655"
     CDS             14009..14719
                     /locus_tag="PK264_07655"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HRD75796.1"
                     /translation="MSDKKKPPADEARSVSERIRSRIEAANARFNANDNISGYIEPGE
                     IDQLVDEVARRMQSVLESLVIDTRNDHNTQDSARRVARMYLHEVFRGRYQAAPPVTEF
                     PNVTGLDELMIIGPITVRSACSHHFCPIMGRLWVGVMPNQHSNLIGLSKYARLAEWVM
                     SRPQIQEEAINQLADLLTDKVKPDGLAVVMEADHFCMHWRGVRDTGAKMINSVMRGSL
                     LTNIALRREFLALINSKR"
     gene            14727..15155
                     /locus_tag="PK264_07660"
     CDS             14727..15155
                     /locus_tag="PK264_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HRD75797.1"
                     /translation="MLVQCLYASRAVGAVSDAVLDDILRQSRRNNLGRGITGMLCHAN
                     EIFLQVIEGSREEVSGLLGRLYADPRHKGLQLLSFEEITQRRFGNWTMGKVNMSAINA
                     ALLLKYSEKAELNPFACTGPATLHLLLEIAEVGAIAHRST"
     gene            complement(15270..>18057)
                     /locus_tag="PK264_07665"
     CDS             complement(15270..>18057)
                     /locus_tag="PK264_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRD75798.1"
                     /translation="EARVLMMSTNNILHPANGSPIIVPSQDIVLGLYYLSIQREKEPG
                     EGMLLGSVAEARHAIEAGAVTLHAKVRGRISTVDANGNDVLEVHETTPGRMILGELLP
                     RKPNVKFALVNQLLTKKAISGLIDAVYRNCGQKETVIFCDRIMSLGFYHAFKAGISFG
                     KDDMQIPSTKTKIVAQTTELVREFEQQYNDGLITQLEKYNKVVDAWSKCTEQIAKEMM
                     DRISAPQVETSGRTKPINSIYMMSHSGARGSPTQMRQLAAMRGLMTKPSGEIIETPII
                     SNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDVSQDCIINEEDCGTDK
                     GIAVQAVVDAGTEIVSLAARVLGRTTAEDVLDPATQKVIVKAGELIEERHTDIIKKAD
                     VQEVRIRSVLTCETTNGVCAACYGRDLARGTPVNIGEAVGVIAAQSIGTQLTMRTFHI
                     GGAANVVDQSFIESNFNGTVKIKNRAMARNSQGQLVATGRSNVVVIVDQGGKELANHK
                     IPYGARLHVDEGDAVKRGTRIAQWDPYTRPIMAEVDGVIDFEDLVDGISVKEQTDDVK
                     GTTNRVVMDWRASPRGASLKPAVVIKEGKNGKPVILARGGDARYLLSVEAVLSVDQGT
                     KVKAGDVLARTPTASAKAGDITGGLPRVAELFEARRPKDHAIIAEISGIVEFGKDYKN
                     KQKILIKPEDPSEASKEYLIPRGKSLAVQKGDRIEKGDFIYDGHPAPHDILAIKGVEE
                     LAKFLINEIQDVYRLQGVTINDKHIEVIVRQMLQKIEIEDSGDTELIKGEQIDKIELD
                     ALNLAFEKEGKRPATGHPVLLGISKASLQTRSFISAASFQETTRVLTEAAVNGRVDEL
                     EGLKENVIVGRLIPAGTGGFLRKLREEAAKRDELIAEKNAKIETDKTLAGPEGAAPTT
                     LAQRRRRRPEDSIQ"
BASE COUNT         3134 a         5720 c         5766 g         3300 t
ORIGIN      
        1 gcgtggcggc gagggggttc tctaatttgc ctttttcatc gtagcgtttc cggcccagca
       61 tctcggcttc cggaccccag tagacgagct ccggctccca gtcgtccgcc cggccaccgg
      121 caaagccgaa ggtcttgaaa cccatagatt ccatggcaac attgccggcc aagaccatca
      181 ggtcggccca ggagatgttg cggccatact tctgcttgat cggccagagc agccggcgcg
      241 ccttgtcgag gttgccgttg tcgggccagc tgttgacggg cgcaaagcgc tgattgccag
      301 tgccgccgcc gccgcggccg tccgccgtgc gataggtgcc cgccgaatgc caggtcatgc
      361 ggatgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnncgctt caggcccgca tagtcgatct tcttgaactc ggcggcgtag
      481 ttgaagcccg cgctcatcgg gttggatgcg ggcgcgtgct ggtggaggat cttcaggttg
      541 agctggttcg gccaccagtc gcggttcgag cgcgcgctcg agcgcgtcgc cggattggtt
      601 ccgtgcatga ccggacactt ggcttcgttc gtcatgatct cttcctctcc tgcttcgttc
      661 gcggcatcgt agggggtggg ggacttgcag tcgtgctttt ctggggccgg cggctcacgg
      721 ccagaggcgt cgagcgagtt ttgccacgac aagcagccat ggcaaggcgt gatagatcag
      781 gtcaaagatg tcgatcggtc gggtcagcgt gccggtggcg agcagcctga gtttttccca
      841 gatgtgcggt tccgggatgt agggcaggag gcccaaggta aggcaaccgc cgatcaccag
      901 ccaccagggg agcttgtcga gccaggccaa caatgtactc atgccaccnn nnnnnnnnnn
      961 ngttcgcgac tgttgtctgc attggagggg cgtctcgcgt gctgtgcacg ttcccaagtg
     1021 cccgtcagcc gtctgcgtgc gtctgcaagg aagcgatctc cagtacgttg tcgcgacgat
     1081 tgtcgatcac gtcgggcaag gccccgaaac cgttctcggc tgccggactg atttagaatc
     1141 attctagatc attggccagc acatttgtcg gtgccacggg agtagcacgt agctaaattg
     1201 cgcgcgcgag gggcgccgcg aactgtcggg ctcgcccgcg gtctcctgcg gtggacctcg
     1261 ggcgagccat tgctgaagtc aggccattca acctggggag ttcagctcga cttttttccg
     1321 ctcgatccga ggctggcaat gaccagcagt gccaacgcgg cggcggcaat gatgccaatc
     1381 gtcatgttcg gtacatagaa cgtgcgatgc acctcccagc cgagcatgcg cagcaccttt
     1441 gcgaggtcgg tgtagatccc ggagagatgg ccgccgagcg tgccggtgac acccaggagc
     1501 ccgctgccga ggagagccag agccgccatg atccagcgcc cgggtccgtc ccagattgcc
     1561 tcaccctgaa cgactcgcgt gacaagcatg agcgcccagt acgccgtcgt ccacacggcg
     1621 agcagcatgt ggttgcggcc catgggcgat gtcgagatcg cctcccatga ccatgcaaag
     1681 cggccgaccg tgatggcgag gagcccgctg atcaggccga cggtcaggag cggcacgagc
     1741 gcccggtcga tcgcgagcgc gaccggtccc ctcgataccg cgcgccacag gatggcgagc
     1801 gtcgccgtca tccagagcgc gatcggaaag tgcacgagca cgacatgata tgcagccatg
     1861 atcaaactcc cttgccgggg ccgagcgtgc ggttcagcgc gatgccgaag gccgcgatga
     1921 ggaggactcc ggcgagcatg gtgagcagcc actgccgctt gatctcggcg tcgtactcca
     1981 tctcgatgcc gtagtgcgtg agctccgcct cgctgtggcg gtggcggaca ggaacgccgg
     2041 ccctcacctt ctcggcgccg gcatgcgccg cactgttgac gtgtccccag gtcacctgac
     2101 ccgggtagaa gagcgtgatc gtctttgcgg gctgatccca cttcggctga tcgccctcga
     2161 agcgtgccgc cgtgatctcg gcagcgcggc cgagcccgag cgagaacggc agtgacacgt
     2221 agtgccagcg gccgcccgtt gcctgccggt gcacggcaaa ggcgacgtga tagatgccgc
     2281 ggtcgaccag aatcttgtcc tcccgcttgt tgccggtatc cattttgcgg gtgagcgtca
     2341 cgtcccagaa gccatccttc caggcggcct tgcccacgac cttgatgtcg gcgcgagaac
     2401 cgtcacctgg ccgtacgatg cggcggggca gcgtatcgcc gtcctgccag ccggcgcccg
     2461 gatcgaacgg catggcgata tcctcgcgga gcgcggaggc gacatcctgc gtgatcttgc
     2521 cgaccttgag atcgtcccac ttgagggcct tgtggccggc cttgcccgcg tcgaacatca
     2581 ggcgcggctg cttcttctcg ccatcccaat tggtcacgta catgcctttg ccggcatcgc
     2641 tgagacgcgc ctcggcaacg agctggtcgt cggcgaggcc gaccgggttc gagcgattgg
     2701 cacgccagtg ccagaggtcg aggaagtagc cggcgttgcg cagcgcattg agctcgtcct
     2761 ccggaacgac cgaggcccag tcatcgatct tgctacgcgt cgcgggcagg tacttcgtga
     2821 gttcgtcctg cttgagcttg gcgccaagat aagggtgctt ggcgacgtcc ttgccgctga
     2881 cgtcgttcgt caggccggcg aggcgatggc cgacggtgat gtaggcgcca tagcgcgaga
     2941 actccggcac gctgccgtcg tcaagcatca tggccacgcg atcttcatgt agtccgtcgg
     3001 gttgcgagcc gggcacggct gagcccttga ccacccattt gccgccctcg aagcgcagca
     3061 tgtcgtggaa gatgccgggg ttcggcgatg gccagcgata gcggaagacg atgtcgcgac
     3121 cgttgtaggc ggcttgcacc gagagcggca cggtgagcgc cgcaggaacc gaccgatggc
     3181 tcgccgggtc gctcgtcacc acacccttgc cgtgcgtcag ccaggagaca gcgagtgtgc
     3241 cgaggaacag ggcgagcgag gtaagaagcc aaggtgttcg cgcttgagac atggatacct
     3301 ccggaggctc gtttcgcccg gggtccttgt cagggttcga caatcaggcc gacgctggca
     3361 gagatcgcgg gccacttggc aggccccgcc atcctagggt ccgctgtagg cgcgatcgtt
     3421 gatgacgatc aaattgacga acgatcggcg ccatatcccg attgaacgat cacatgccct
     3481 tcgcgtcgct ccgccggatg cgcatgccct tgccggtctt gacgccgggt tgcagggcga
     3541 ggccgaggcc cgggcccttc ggcaccgtga tcatgccccg ctcgacgggc ggcagctcgg
     3601 tgacgaagcg gtggtaccag ccgtagtaga aggcgcgcac catctcttgc gtccagcaat
     3661 tgcgggagtt gagcgcgagg tgcgtcgaga cggcgagcac cacggggcct gtgcagtcgt
     3721 ggaaggcgac cggcacgtga taggtctcgg ccatggtggc gaccttcctc gcctcgctga
     3781 tgccgccgca ccaggagagg tcgaggatca ccatcgagag cgcctcgagc tcgagcaggc
     3841 cgcggaaatc ggcccggccg ccgcgcgtct cgccgacggc gatcggcgag cgcgtcgcgc
     3901 gtgcgacttc gccgatcgaa tggaggtggt ccatgaacac ggggtcctcg acccacatgc
     3961 acctgatggg ctcgagcgcg cgggcgatct tcaccgcggt cggccggctc cagagcgagt
     4021 gcagctctgc ggcgatctcc atcttgtcgc cgacggcttt cctgatcttc tcgaaggggg
     4081 cgaggccgga cttcagctcc tcggccgtga tgtcgtagcc gctcgagcgc tcggcggcgt
     4141 agtcgaacgg ccagatcttc atggccgaga tgcccatctc gagaaggctt tcggcgagct
     4201 cgcccgcgcg gttcaaaaat gcgtcgaggt cctcgtactg gccttttctg gcatcgagcc
     4261 cgaagttggc ggttccctgc gtgaccttgg tctgcacgta ggagtagccg gcgcaggtgt
     4321 tgtagacgcg gatcttctcg cgcacccgtc cgccgaggag atcgcagagc ggcagcttca
     4381 cggattggcc gaggatgtcc cagagcgcga tgtcgatcgc agagcggccg cgcatctcgg
     4441 cggacgagcc gacgaagccg acgtagcccg taaggaacgc ctggtgcgcc tcgatgttgc
     4501 gcgggtcctt gccgaggagg tagggcgcga tgatgccgtg gatgtgccct tccgcggcat
     4561 ccgggccata aaacgtctcg ccgagaccga cgatgccttc gttcgtcatg acctcgaccc
     4621 agaggatgtt cgggaactcc tcggagcgaa tggtctctat tgccgtcacc ttcattggaa
     4681 ttttccccta gaggccgggc tcgtccctga ccatgccatg cgatcacgat atcgatcgcg
     4741 agcatcaccg ccgcgccgcc gcttccagca atctggccga caatcctgcc gaggtcgagc
     4801 ccgctcgtcg ctgagcctgg cgttgcagat cggcgacacc cggctcaatt cgaggggcgt
     4861 cgcatcgccg tttagcgatt ggttcaccaa ttgcggtagc atccgtccag cgaacgagcg
     4921 tcgagaggtg atctcatgtt gaagtcgatg atggcagcac ttgtcgcggc tggtgcggtg
     4981 acgattgcgg tcgcagcaga tgcatccgct catgagcgcc gagcgtcccg gacgacggat
     5041 tccggcgcgg tcctcacgga gcatccgaag gccggcacct atcgccgcgg cccgcgcgtg
     5101 aagggtttcg tcgcccgtcg cggcggctat tcctacagct ggtttgattc gatcaacacg
     5161 tatggctcgt cgcgcacggt ctatggcggg acgtttctct atcgcgaccc gatgatggat
     5221 cgccagactg gggcggggcc gttcgaccac ggcttcttct tcgattcagg tgtcggcccc
     5281 cgcggcggcg acagcccata catgcactga agggccgcca gttccttcgg caagatcaag
     5341 gtcgccaggg tgccggcttc tcggcaccct cggcggcctt ctcttttgtg gtcgatcgct
     5401 tgatctcgcg cgccagggcg tagagcacct cgtcgatgcc ggcgcctgac acggccgaga
     5461 tgagcaacgg ctgcttgcgg gcggcgcgct tcagggcttc ttcccgctcg gcgatcgtct
     5521 cttcatccag ggcatcacac ttcgtgagcg cgacgagctc cttcttcttg tcgaggcctg
     5581 cgccgtaggc cgccaactcg cgacgcacta cgcgatagtc ttcgaccacg ttttcgctgg
     5641 tcgcatcaac gaggtggagg acgacgcggc agcgctcgat atggccgaga aagcgtgtgc
     5701 caaggccgac gccttcgtgc gcgccctcga tgaggccagg caggtccgcg agaacgaagt
     5761 cgagcccgcc gatgcgcacc atgccgagat tcgggtggag cgtcgtgaag ggatagtcgg
     5821 caatcttggg cttggcagca ctcaccttgg cgaggaacgt ggacttgccg gcattcggca
     5881 gcccgacgag cccggcatca gcgatgatct tgagcctcag ccagatcgtc agctcctcgc
     5941 caggaaggcc ggggttggcc cgccgtggcg cacggttggt cgccgacttg aagtgcgcgt
     6001 tgccgaagcc gccattgcca cccttggcga tgagtgcggt ctcgcccggc accttgaggt
     6061 cggcgagcag cgtctcgttg tcctcggcaa agatttgcgt gcccggaggc agcttgagcg
     6121 tgacgtcgcc cccatcggcg ccggagcggt tgcggcccat gccgggcttg ccgttgccgg
     6181 ctttgaagtg ctgctggtag cgatagtcga tgagcgtgtt caaattgtcg acgcactcgg
     6241 cccagacatc gccgccccgg ccaccgtcac cgccgtcggg tccgccgaac tcgatgaact
     6301 tctcgcgccg gaagccgacg cagccgttac cgccgtcgcc cgagcgaatg tagatcttgg
     6361 cctgatcgag gaatttcatg gtttgccctt cgcaacgcga cgacgaccag accgcacgcg
     6421 ccttcgcagg atgccaaatc gtcgggcgcg cgcaaccgcg attgtcgcga ttcgggatca
     6481 atagagcaac attggcccat ggtcatctac aggcgcagag ttggcatcgt tgcaatcatc
     6541 ggttgctctg gtgagattgg ttgcggcttc catcaaatgg atgatgcgct tactgctgtt
     6601 tccgatctag attggacttg tacgaattga ttttcgcacc ttgctccagg agtagccggc
     6661 atgcctatcg tccagatttc acagtcatcc attctcaatg gtgtgacctc aggcgatcag
     6721 accgatcccc agacgatccg ctgggaaggg ggcagcgttg ccacctacac gtcggtggag
     6781 ggtggcgtcg ccgtcattcg gctcgcaacc ttcaaccacg agggcgagcg aatcggcagc
     6841 gacatcgtcg tctcgcaggt gccgagcacc gacaatatgt cgacggcttt cctcggccga
     6901 ctgagcaacg gcacgatcat tgtgacgtgg gtcgatgtca cgacgggcga agccttcttc
     6961 cgggcctacg acggcacgac gctcgccccc ctcaatgcgc cgaccgagat cacacagaca
     7021 tctgctgtgg acgcgacctt ctccggcatc ggcttcgccg aggccgccaa cggcaatgtc
     7081 ctcttcagct ggaccacgac cgaggcaagc ggcgacgggg ctggactagc cgtccatgcg
     7141 aggatgctgt ctgccgacct gtcgaccttc gagagcgagt tccagctcaa ctcgaccacg
     7201 acaggagatc aagctcgggc cagggtctct cagctcggca acggcaattt cctcgtaacg
     7261 ttccaaacga acgacagcgg cacggacgac atccgtggtc gcatcatcga tcagaatggc
     7321 gattccgtcg gcaacgactt cgttatcaac ggcacgacgg cgggtgatca gcagcgtgca
     7381 cgggctcggc agctcgacga cggacggacg ctgatcgtcc atgaatcgaa tgacggcggc
     7441 agcgacgtca acatccgcat gcggctactc gacctcgacg gcgtgccgat cggcgcagac
     7501 gagatcgtca acacaacgac aactggcaac caacgtgcgc ccaatctgat tgtccttccg
     7561 gatgggcgcg tcgcaatctc ttggtcctcg ttcgaagtct ccggtagtgg tggcacgcag
     7621 gtggtcatga gggcccgaat ctgggacccg gacggcctgt tcccggaaag cgatttcaac
     7681 ctcggtcagt tccccacgac caattccatc cccttcacga gccttcgcgc gaccgaggac
     7741 ggcaggctcg ttctggccgt cggcacgccg agcctctcgc ttgatggcag cggcctcagc
     7801 acggagttgc gctacttcgt cccgacaacc gatcttgacg aggaggtcgc aaacggtacg
     7861 actggtcccg accccgcgct gatcggcaca agcttgacca cgagctggat cgatggtctg
     7921 gccggtgatg acagcattga aatggccggc accggacggg cccacttcta tggtgatgcc
     7981 ggcaacgata ccctgatcgg cagctggaac accgatactt tgtacggcgg caccggcaac
     8041 gatcagctct cgggcgccgc aaatgcggac atactagccg gagagaccgg caacgacatg
     8101 ctcgacggcg gcgctggcgg cgacgtgctc gatggcggcg acggcttcga cttcgtctcc
     8161 tatctctccg cgacgtcggg tttgaccgtg aacttgagca gcccggatct caacacgggc
     8221 gatgcgttcc tcgatgccta cgaaggcatc gagggcctcg tcggctcgag cggcaacgac
     8281 aacctcacgg gcacctccgg aaatgacgtc atctatagcg ccctcggcaa cgacatcatg
     8341 tacggcctgc agggcaacga cacgctgctc ggccaggccg gcagtgacat cttcttcggg
     8401 ggcgaaggca acgacttcat gagcggcgtc aacggcgcgg aggtggacgt caacatcttc
     8461 gtgttcaacg tctcgcagta ctatggcttc ggccaggata cgatcgtcgg cttcgatgcg
     8521 aacgacgcga gcccgggcca cgacgtgctc tacatctcat ccgcagtctt cgcggatttc
     8581 gcggccatgg aggcgtcgac gttccagttc ggaacggcga cggtgatctt ccggcccggg
     8641 tcctcggatt ggatcaagct ggtcggtgtc gacaagacca cgctcacggc tggcgacgtc
     8701 gtcttcttct gaggcccgca ttgagctcag gacactcccg gcgcggcatt ggcgtccgcg
     8761 ccggttccgc ctatggtcga tcggcagcaa ggccgatcgg cgatacccgc ggaggtgacc
     8821 atgagcaaca agatgcgttt ggcgatcctc ggcggagcgg ggcggatggg ccgtgaactc
     8881 gcacgggcca tcgcggcgag cgacggcgcc gtagtcgccg cggccatcga gcctaagggt
     8941 tcggccagca tcgggcgtga tgctggcgac ctcgcagggc tcggcgcgag cggggtcgag
     9001 atcaccgatg acctcgcaca ggtcgtcggt gcgatcgacg gcgtcctcga cttcacgatt
     9061 ccgaaagcga cctgcgcggc tgccgccatc accgccaagg ccggtctcgt gcacgtcatc
     9121 gggaccacgg gcctgtcggc ggcggaggag gccgagatcg cgaccgcggc catgcgctcc
     9181 gtcatcgtca aggccggcaa catgagcctc ggcgtcaatc tgctgacggc gatcacacgc
     9241 aaggttgctg ctgcgctcga tcccgacttc gacatcgagg tgctcgagat gcaccacagg
     9301 cacaagatcg acgcgccttc gggtaccgcg ctgatgctcg gccaggcggc ggcagagggg
     9361 cgaaatgtga cactcacctc ggtcagcgtg cgttcgcgtg acggccacac gggggcacgg
     9421 cggcgcggcg acatcggctt tgccacgctc cgcggcggca gcgtcgtcgg cgatcacacg
     9481 gtgatcttcg ccggcgaggg cgagcggatc gagatcacgc acaaggctgc cgatcgcggc
     9541 atttttgcgc ggggcgcagt gaaggcggcg ctctgggcgc gcggtcgaag gccgggcctt
     9601 tataccatga ccgacgtgct cgggctgtga gcaatgcagg tcgctgtcga aacccaaatc
     9661 atcgcgccac ccgagaccgt gttcgacacg ctcgccgaca tccagcgctg gcccgagttc
     9721 atctccgcga tcgaggcgat cgagctgctc aatgccgaca cgttcgccca aggccttcgc
     9781 tttcgcgaga cgcgcatcat gttcggccgc aaggcgagcg aggagatgac ggtcgtgacg
     9841 ctcgagcggc cacgtctcat ggtgttcacc gccgagagtc atggcacgcg ctatcgctcc
     9901 gagcaccgaa ttgcaccgga ggggacgggc acgcacttga cgctcatgtt cgaaggccgt
     9961 ccggnnnnnn nnnntgatgg cgcgcctcat gacgccgctc gcgctcgtgc tcaccggcac
    10021 gatccggaag cagctcgctg ccgacctcgc ggatgtcaaa cgtgaggccg aaaggcgagc
    10081 agcgtcggcc tgatcgaacg ctgcaagact gcccccgtac aacccgagga aggcgatcat
    10141 gaccgcacac cagaccgcaa aggctcaagc gttccgcgcg ctccatggtc gcggcagcgc
    10201 cttcatcatt cccaatcctt gggacgtgag ctcggcgcga attctcacgg gtctcggctt
    10261 cgaggcgctg gcgacctcga gcggcgcggc agccggtaca tttggccggc gcgatggggg
    10321 cattacccgg gcgcagtcgc tcgccaatgc ccgcgccatt gtcgaggcga ccgatctccc
    10381 cgtcgcggcc gatctcgaga acggcttcgg acacggggct gacgaggtcg cactcaccat
    10441 ccgcgaggcg agcgggatcg gtctcgtcgg cggctcaatc gaggattgca ccggcggcaa
    10501 ggacggcggc atctacccaa tcgacgtcgc cgtcgctcgc atcaaggcgg ccgcagctgc
    10561 ggtgggcgcg ttgccagtgc cgttcgtgct caccgcgcgc gccgaaaact tcattcgcgg
    10621 ccggcccgac ctcgacgata cgatcgcccg gctcaaggcc tacgaggcgg ccggcgccga
    10681 ggtgctgttc gcgcctggcc tccccgacat cacagccgtg aagcgcgtct gctccgcact
    10741 cacgaagccg ttcaacttca tggtcggcat caaggggaag tcgttctcgt ttgcggagct
    10801 cgagtcggcc ggggtcgtgc gggtcagcct cgcgacgtcg ctctatcgcg cggccatgac
    10861 gggtctcatc gaggcggcgc gagaggcgcg cgataagggc acgttcggct tcgtcgacag
    10921 gtcactcccg acgcccgata tcaacgcggc tctcaaggcg tgacggagaa tgcccgcagg
    10981 attatctgcc agccggccga gcggcgaatg atgaaaagac aggcaagagg cctatttgat
    11041 atgcagtaat tagcccagtc agcacaacgc gtcctgcctc agaattcagc accgacattc
    11101 caatcgattg aagagaaaca caaaatttga gcgtcgcgag tttggcatgt ttcgtgctgt
    11161 gagtctggat agttaacacc gcgttgcacg cagtaagggc cgtcccgcat gaagaagatc
    11221 gaggccatca tcaagccatt caagctcgac gacgtgaagg aggccctcca ggagatcggc
    11281 ctgcagggca tcacggtcat cgaggccaag gggtttggcc gccagaaggg ccacaccgag
    11341 ctctatcgcg gcgccgagta tgtcgtcgac tttctgccga aggtgaagat cgagatcgtg
    11401 ctcgccgacg agatgctgga gaaggcgctc gaggcgatcc agcgcgcggc caagacgggg
    11461 cgcatcggcg acggcaagat cttcatctcg accatcgagg aagccattcg gatacgcacg
    11521 ggcgaaaccg gcgccgaggc catctgacac ctgacccgtt tcatacgtcc tcggtgctat
    11581 ccgccaagct gctattcgca gtccctggca gcgcagcgag cggcgtgcga aagcaatgcc
    11641 ctgaaaacca acagatctga catcagccaa cgcagaggac aatcatgccg accatcaaga
    11701 cacctgccga cgtcctgaag ctcatcaaag aaaaggacgt gaagttcgtg gacctgcgct
    11761 tcacggacac caagggcaag ctgcagcacg tgacggccga tgtctcgtgc gtcgatgagg
    11821 ccatgttcgc cgagggctat gccttcgacg gctcctcgat cgccggctgg aagggcatcg
    11881 aagcctctga catgacgctg atgcccgacg cgtcgagcgt gcatctcgac ccattctttg
    11941 cccagaccac gctcgccgtg ttctgcgacg tgatcgagcc ctcgaccggc gagccctacg
    12001 agcgtgaccc ccgctcgatc gccaagcgtg cggaggccta cctccagcag accggcatcg
    12061 gcgaccgcgt ctatgtcgga cccgaggcag agttcttcat cttcgacgac gtcaagttct
    12121 ccgccgaccc gtacaatacc ggtttcaagc tcgacagctc ggagctgccc tcgaactctg
    12181 ataccgacta cgagatgggc aaccttggcc atcgtccgcg caccaagggc ggctatttcc
    12241 cggtgccgcc tgtcgacagc tgccaggaca tccgttccga gatgctctcg gtgatggcgg
    12301 agatgggcgt cgtcgtcgaa aagcaccacc acgaggtggc gtccgctcag cacgagctcg
    12361 gcatcaagtt cggcccgatg gtgacgatgg ccgaccacat gcagatctac aaatacgtga
    12421 tccacaacgt cgcccaggcg tacggcaagt cggcgtgctt catgccgaag ccgatcttcg
    12481 gcgacaacgg ctcgggcatg cacgtgcacc agtcgatctg gaagggatcg acgccaacct
    12541 tcgccggcaa caagtacgcc gacctgtcgg acacctgcct gcactacatc gccggcatct
    12601 tgaagcacgc caaggccatc aacgccttca cgaacccgac gacgaacagt tacaagcgtc
    12661 ttgtcccggg ttatgaggcg ccggtgctgc tcgcctactc ggcgcggaac cgctcggcct
    12721 cctgccgcat cccgcacgtg acgaacccga aggcgaagcg ggtcgaggtc cgtttcccgg
    12781 atccggcggc gaacccctac ctcggcttcg ctgcgatgct gatggccggc atcgacggca
    12841 tccagaacaa gctcaacccc ggcgaggcca tggacaagaa cctctatgac ctgccgccag
    12901 ccgagctcga gaagatcccg acggtgtgcg gcagcctgcg cgaggcgctg aacagcctcg
    12961 acaagggccg agacttcctg aaaaagggcg gcgtcttcac cgacgacatg atcgacgcct
    13021 acatcgaact caagatggcc gaggtcgagc gcttcgagat gaccccgcac ccggtcgagt
    13081 tcgatatgta ttactcggtc tgatgagcgg catatccgtc ccgcgcacca atatcggggc
    13141 ggcccactcg ggccgcccct cgttctttct ctcgtcgatt cgagaccggc gaatatggtg
    13201 aacgagaccc acaatcggcg tcctcgttgt cagggcgtct gcatcttgat aacttcatcg
    13261 catgtctgag cttccagcca ccacgcgcgc tgcggatggt tggccacgca tggcgtggac
    13321 gcatcacgaa ctcgatcgca tgagcgaggc tgggcttttt gcgcccggtg aacgcgtaga
    13381 gctgatcgat ggcgagatca tcccgatacc ggccaaaggt cagcgccacg atcgcgtgcg
    13441 gatggcgctc aatcgctcga tcagccgtcg gctcggcgag gacaaggacc tactgccgga
    13501 gcctggctgg aggccggccg agcggttcta tttcgagccg gactttctga tcgtgccccg
    13561 gtccgtcatc gtgccggagt tcgctaggac tgaagtgctt cttggcattg agattgccga
    13621 ttcgagtata tgctatgaca ccacgatcaa gcgggatcgg tatgccggcc tcggtgtggt
    13681 cgaatactgg gtagtcaacg cctgagagct gtcgacgatt gtccatcgcg agcctgacgc
    13741 cggtgtctat cggctgatcg aggcacggcc ggcagcgacg ctgctgatgc cggatcgcgt
    13801 gcccgagatc gcactccgcc ttgctgatct aggcgcttcc tgacgccann nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggccgt tcatcacgtc acgcaagggg
    13921 cgtccaaggc gattggcggc tgagttgccg ttgactatcg tgagcccgtg gccgcagtcc
    13981 atggcacccg gatcagtcag gaggaaaggt gagcgacaag aagaagccgc cggcggacga
    14041 ggcgcggtca gtttcagagc gcatcagatc gcgcatcgag gccgccaatg cccgcttcaa
    14101 cgccaacgac aatatttcgg gctacatcga gccgggcgag atcgatcagc tggtcgacga
    14161 ggtggcgcgc cgcatgcaga gcgtgctcga gagcctcgtc atcgatacgc gcaacgatca
    14221 caacacgcag gattcagccc gccgcgtcgc gcgcatgtac ctgcacgagg tgttccgtgg
    14281 ccgctatcag gcagcgccgc ccgtcaccga gtttccgaac gtgaccggcc tcgacgagct
    14341 gatgatcata ggcccgatca cggtgcgcag cgcctgcagc caccatttct gcccgatcat
    14401 gggtcggctc tgggtcggtg tcatgccgaa ccagcattcg aacctcattg ggctctcgaa
    14461 atacgctcga ctcgccgagt gggtgatgag ccgcccgcag atccaggagg aggcgatcaa
    14521 tcagctcgcc gacctcctca ccgacaaggt gaagcccgat ggcctcgccg tcgtcatgga
    14581 agccgaccac ttctgcatgc actggcgcgg tgtccgcgat accggtgcca agatgatcaa
    14641 cagcgtgatg cggggctcac tgctcaccaa catcgcgctc cgtcgcgagt tcctcgcgct
    14701 catcaactcc aagcggtgac acgccgatgc tcgttcaatg cctctatgcc agccgtgccg
    14761 tcggcgccgt cagtgacgca gttctcgacg atatcctgcg ccagtcgcgc cgcaacaatc
    14821 tcggacgcgg catcacaggc atgctctgcc atgccaacga gattttcctt caggtgatcg
    14881 agggcagccg cgaggaagtt tccggactgc tcggccgcct ctatgccgac ccgcgtcata
    14941 agggcctgca gctcctctcc ttcgaggaaa tcacgcagcg ccgcttcggc aactggacca
    15001 tgggcaaggt caacatgtcg gcgatcaacg cggcgctgct gctcaagtat tccgagaagg
    15061 ccgagctgaa cccgttcgcc tgcacgggtc cggcgacgct gcatctgctg ctcgagatcg
    15121 ccgaggtggg cgccatcgcg caccgcagta cgtgatgcgg gtcgcgttcg cgtccttgag
    15181 gtcgatcaac acgaagaagg ccgctcccgg atcggggcgg ccttcttctt ggtcgatctc
    15241 acatcctcgc gccgaccgcg tggacgccgt tactggatcg agtcctcagg gcgacgacgg
    15301 cggcgctgcg ccagcgtcgt cggcgcagca ccttcaggcc ctgccagcgt cttgtccgtc
    15361 tcgatcttcg cgttcttctc ggcgatgagt tcgtcgcgct tcgccgcctc ctcgcggagc
    15421 ttgcgcagga agccgccggt gcccgccggg atgagccggc cgacgatgac gttctccttc
    15481 aggccctcga gctcgtcgac acggccgttg acggccgcct cggtgagcac gcgggtcgtc
    15541 tcctggaacg aggccgcgga gatgaagctg cgcgtctgca gcgacgcttt cgagatgccg
    15601 agcagcaccg ggtgacccgt cgcggggcgc ttcccttcct tctcgaaggc gaggttcagc
    15661 gcgtcgagct cgatcttgtc gatctgctcg cccttgatga gctcggtgtc accggagtct
    15721 tcgatctcga tcttctgcag catctggcga acgatcacct cgatgtgctt gtcgttgatc
    15781 gtcacgccct gcagccggta gacgtcctgg atttcgttga tgaggaactt ggcgagttcc
    15841 tcgacgccct tgatggccag gatgtcgtgc ggcgcggggt ggccgtcgta gatgaagtcg
    15901 cccttctcga tgcggtcgcc cttctgcacc gcgaggctct tgccgcgcgg gatcaggtac
    15961 tccttgctcg cctcggacgg atcctcgggc ttgatgagga tcttctgctt gtttttgtag
    16021 tccttgccga actcgacgat gccggaaatc tccgcgatga tcgcgtgatc cttcgggcgg
    16081 cgcgcctcga agagttcggc gacgcgcggg agaccgcccg tgatgtcacc agccttcgcg
    16141 gatgccgtcg gcgtacgcgc gagcacgtcg ccggccttca ccttcgtgcc ctggtcgacc
    16201 gagagcacgg cttcgaccga gaggaggtag cgggcatcgc cgccgcgggc gaggatgacg
    16261 ggcttgccgt tcttcccctc cttgatgacg acggccggct tcaggctcgc cccacgggga
    16321 gacgcgcgcc agtccatgac gacgcggttg gtggtgccct tcacgtcgtc ggtctgctcc
    16381 ttcacggaga tgccgtcgac gaggtcctcg aagtcgatga cgccatccac ttcggccatg
    16441 atgggccggg tgtaggggtc ccactgcgcg atgcgcgtgc cgcgcttcac cgcgtcgccc
    16501 tcgtcgacgt ggaggcgggc gccatacggg atcttgtggt tggcgagctc cttgccgccc
    16561 tgatcgacga tgacgaccac gttactgcga ccggtcgcga cgagctggcc ctggctgttg
    16621 cgagccatgg cgcggttctt gatcttcacc gtgccgttga aattcgactc gatgaacgac
    16681 tggtcgacga cgttggccgc gccgccgata tggaacgtgc gcatcgtgag ctgcgtgccg
    16741 atcgactggg cggcaatgac gccgaccgcc tcgccgatgt tgacgggcgt gccgcgcgcc
    16801 aggtcgcggc catagcaggc ggcacagaca ccgttggtcg tctcgcaggt cagcaccgag
    16861 cggatacgga cctcctggac gtcggctttc ttgatgatgt cggtatgccg ctcctcgatg
    16921 agctcacccg ccttgacgat caccttttgc gtcgcagggt cgagtacgtc ctcggcggtt
    16981 gtccggccga gcacgcgcgc tgcgagcgag acgatctcgg tgcccgcatc gacgacggcc
    17041 tggacggcga tgcccttgtc ggtgccgcag tcctcctcgt tgatgatgca gtcctgcgaa
    17101 acgtcgacga ggcggcgcgt cagatagccc gagttggcgg tcttcaaggc cgtgtcggcg
    17161 agacccttgc gggcgccgtg cgtcgagttg aagtactcga gcacggagag accttccttg
    17221 aagttcgaga tgatcggcgt ctcgatgatc tcgcccgacg gcttcgtcat caggccgcgc
    17281 atggcggcga gctggcgcat ctgggtcggc gagccgcggg cgcccgagtg gctcatcatg
    17341 tagatcgagt tgatgggctt cgtgcgcccg ctcgtctcga cctgcggcgc cgagatgcga
    17401 tccatcatct ccttggcgat ctgctcggtg cacttcgacc aggcatcgac gaccttgttg
    17461 tacttctcga gctgcgtgat gaggccgtcg ttgtactgct gctcgaactc gcgcacgagc
    17521 tcggtcgtct gggcgacgat cttcgtcttg gtggacggga tctgcatgtc gtccttgccg
    17581 aacgagatgc ccgccttgaa ggcgtgatag aagccgaggc tcatgatccg gtcgcagaag
    17641 atcactgtct ccttctgacc gcaattgcgg tagacggcgt cgatgaggcc ggagatcgcc
    17701 ttcttggtga gcagctggtt gacgagcgcg aacttcacgt tcggcttccg cggcaggagc
    17761 tcaccgagga tcatgcggcc gggagtcgtc tcgtgcacct cgaggacgtc gttgccgttg
    17821 gcatcgacgg tcgaaatgcg gccgcggacc ttggcgtgca gcgtgacggc gccggcttcg
    17881 atcgcgtggc gcgcctcggc gaccgagccg agcagcatgc cctcgcccgg ctccttctcg
    17941 cgctggatcg agaggtagta gagaccgagc acgatatcct gcgacggcac gatgatcggc
    18001 gagccgttgg cgggatgcag gatgttgttg gtcgacatca tgaggacgcg cgcttcg
//
LOCUS       DAORNH010000054        18001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2383_length_18001_cov_2.976374, whole genome shotgun sequence.
ACCESSION   DAORNH010000054 DAORNH010000000
VERSION     DAORNH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18001
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2383_length_18001_cov_2.976374"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>533)
                     /locus_tag="PK264_07670"
     CDS             complement(<1..>533)
                     /locus_tag="PK264_07670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD75799.1"
                     /translation="TLWLGSSELADEQKIARHLKLVASDLDITLRIIRQNQPGKRVAI
                     ALILRAMSRAGLAKDDLVIFMDGDFVYHPGLVRKSAPLFACDPKLHALTTDEHVIVRG
                     PWWMQSWLNMRFAQRRMAMQSHALSNRVLTLTGRCSIFRGTHIASDEFIRLQEADYLH
                     HWLWGTFRFLSGDDKST"
     assembly_gap    534..633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<634..935)
                     /locus_tag="PK264_07675"
     CDS             complement(<634..935)
                     /locus_tag="PK264_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75800.1"
                     /translation="MYEQLIRESDPTVKLSFTEKWSPLVQMKIAAFFAVCFVVMSYVP
                     NTIWDPEVQDFIYVIGILGIWRYLWWFNHWIRAKVFEKVTYPAMAERAARLWESGWG"
     gene            complement(947..>1782)
                     /locus_tag="PK264_07680"
     CDS             complement(947..>1782)
                     /locus_tag="PK264_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRD75801.1"
                     /translation="LQSFAAIDLKGAEQIRIEIDGLQSQLALAEAQLGRLTSLHKKGY
                     ATDTKVEEAQKAVIALRQVIEGKRVELKSRVELAGANIGKRHYTGANIVGDVGLIEAQ
                     VRLAEHEIQLFHQRFLALANHRARLAVTAPFDGTVLELPHVDNGHVRRGDVIAVIEQR
                     AKRHVLAFLTQDEVMKVGLGDEVTLFIAAVGETLKGRVTGIDRTTGFIAEQNARTAPG
                     YRWRGSVDRSAKVTITFDDPTRVADAERYRSGTPVIAIFPQRSTNSLMASIRNRISRA
                     M"
     assembly_gap    1783..1806
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1807..2583)
                     /locus_tag="PK264_07685"
     CDS             complement(<1807..2583)
                     /locus_tag="PK264_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRD75802.1"
                     /translation="MAVKIKRERPDQRRHHRVTAPMYVHIDGRRYRATDWSLGGLRLD
                     GVEGELPTIGSELALQLTLPFQGFDVTFDAKVEVMRFDADARMIGCRFTELGEREREL
                     MSHFIEELVRGTMVDVEDTIQRIDVPVTPASLQPDASPPKAVPVRRRPSKALVMSAVY
                     AVLGFLVFGYTGLLLYSNFYRLEVQTAVIASPVESVASQAEGRIKTGSLKPGDTVRSG
                     ESIVTLVDNQLEREIELAEIAVKEQKAKLIYMKRRHADELE"
     gene            complement(2752..3108)
                     /locus_tag="PK264_07690"
     CDS             complement(2752..3108)
                     /locus_tag="PK264_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75803.1"
                     /translation="MTARYLEANGIPTVIIGSARDIVEHCGVPRFVYSDFPLGNPCGH
                     PWQPTMQDAIVRLALGQLETAAGARSFVRSPFDWKGGPEWRERYNQVRPEDREKLLAI
                     GDARRLKRGQAPRTSK"
     assembly_gap    3311..3342
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3343..3622)
                     /locus_tag="PK264_07695"
     CDS             complement(<3343..3622)
                     /locus_tag="PK264_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HRD75804.1"
                     /translation="MSEPSTERSVPYMERTRLYYRALGYTNDYVWAHFDDVPFTKLGK
                     PLAAARVALVTTAGPPDRSNRDAKGRKQVWSGDIAAPPAGFDTDLAWDR"
     gene            3813..4295
                     /locus_tag="PK264_07700"
     CDS             3813..4295
                     /locus_tag="PK264_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD75805.1"
                     /translation="MNVMSTPTVKPMAPDLAEIAASTAELCIATRVRQLSRIVTRVYD
                     DALRPLGITASQYTLLTQLANRDGITAAEIGATLDIEKSTLSRNLKRLLALGLINMDP
                     PAGRRGRGLHLTPRGQAVIKDAYPIWSAAQKRSTEIMGGESRAMLDTLLMQAERLIPA
                     "
     gene            4427..4930
                     /locus_tag="PK264_07705"
     CDS             4427..4930
                     /locus_tag="PK264_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRD75806.1"
                     /translation="MTEAASLLEVLRQRTLKIVTAESCTGGLIAGLLTEIPGSSDVVE
                     RGFVVYSNDAKMESLGVPGHLFADHGAVSRPVAEAMASGALMNSLADVSIAVTGVAGP
                     GGGTALKPVGLVHIAAATLGGQPLHQECRFGDIGRSAIRLETIKVALALVRRLTIDMP
                     LAPPADA"
     gene            complement(4964..6157)
                     /locus_tag="PK264_07710"
     CDS             complement(4964..6157)
                     /locus_tag="PK264_07710"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRD75807.1"
                     /translation="MSKTADPIVIVSGARTPIGGFQGALSSLPAPELGAVAIKSAVAR
                     AGVKPDEIAEVLMGCVLPAGQGQAPARQAARYAGLPDSVPCTTVNKMCGSGMKTVMMG
                     FEALHVRAGEVIVAGGLESMTNTPFILPKMRSGQRLGHTQVYDHMFLDGLEDAYDKGR
                     LMGTYAEDTAQHYQFTREAQDAYAIESLRRAKAANEDGSFDAEIAPVTVKSRKGTSEM
                     KRDEQPFTADPAKIPTLKPAFRDGGTVTPANSSSISDGAAALVLMRESEAKRRKLEPL
                     ARILATASHAQAPAWFTTAPIGSIKKVLAAAGWSAKEVQLYEINEAFAVVTMAAMRDL
                     DLPHDKVNVHGGACALGHPVGASGARIIVTLMNALKKRGETKGVASLCIGGGEATAVA
                     IERLA"
     gene            6416..7150
                     /locus_tag="PK264_07715"
     CDS             6416..7150
                     /locus_tag="PK264_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75808.1"
                     /translation="MSVFEDIIEATEHPELAGKRVLVTGLTMALGVDVARAFAEHRAR
                     LVLHIAEDGPEADALASYVSSTALDLELYSGAIGDKDAATKLAQRASTAFGPLDVIVN
                     LAVIATPRTGDAATEAEIEDAVSSGLRAPLVVTRIAANRMRLTMTGGLILNVAIVTGP
                     DSKARRAVAAVTRTTLAAMTRAEAASCAADGIRINAIAPRSGLFEHERHLQNEPQIAN
                     VALYLASKKGREHTGLVFEANGPQCR"
     gene            complement(7171..8013)
                     /locus_tag="PK264_07720"
     CDS             complement(7171..8013)
                     /locus_tag="PK264_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRD75809.1"
                     /translation="MTNRKLAYIVKDQKPLVLAESETAGRACALMCEHGAGSVLVVGK
                     EHHLTGIFTGRDAVRLIAKGRAPGEVKLAKAMTEGPVSVRPDCTAIEALKAMAAGGFR
                     HVPVTDDHEIVGVVSKGDFKGMEFEAFLWRELGGGLGNSADRRVGDIIEGKTPLIAKS
                     DETVKHVCQTMLKESRGFALVTDACGHLKGIFTGRDAVKAIRSGIAPEATAVSKAMTG
                     KPVTIASDARAIEALRAMCDGGFRHLPVVAEGKVLGVVTRADFTGYEIDQLDEQEHLR
                     ECIW"
     gene            complement(8075..8575)
                     /locus_tag="PK264_07725"
     CDS             complement(8075..8575)
                     /locus_tag="PK264_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD75810.1"
                     /translation="MAYRKAGDVPRAIEDFSAAILLNPVYAVAYANRGYAYETQGKRA
                     EAIQDLQRALLFDPSHIGARDGLARLRSSAPLAEEAAKRVGEGAALVETHCKSCHAIG
                     REGASPKAGAPAFRTLHARHPMEALREPLTRAIAAPHDEMPKFVLADDHIDRIVAYIN
                     SLDGAN"
     gene            8745..9059
                     /locus_tag="PK264_07730"
     CDS             8745..9059
                     /locus_tag="PK264_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75811.1"
                     /translation="MVAAPHPHEATKDVGLNVSAAEPDRLVEVGDREIKDSGLRALAA
                     TIAIGNGLLLDGVSGPRDHVAAGRELLFGAGRSTASLERIRAGATCGTPHEGSGKHQC
                     IE"
     gene            complement(9189..10079)
                     /locus_tag="PK264_07735"
     CDS             complement(9189..10079)
                     /locus_tag="PK264_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD75812.1"
                     /translation="MGNLEVRDRRLTEVVGSDVSFEKLGTGFKFTEGPLWHPVEGHLT
                     FSDMPGDIMRRWDAKGGVRTFRQPTHKSNGLVYDKSGRMLICEHASSRVTRLEADGST
                     TTLASHNGDTELNSPNDIIVRNDDAIYFTDPTYGRMDGFGLKRPQALSFQGVYRIDPG
                     TGAVRCLADDFKQPNGLCFSLDEQRLFVSDTERQHIRVFDVRDDGTLANGRVWAETRG
                     EGAGAPDGMKIDAIGNVYSCGPGGIHVFDPDGRALGVIRVPEYTANFCWGDGDYRSLY
                     ITASTSLYRIRTRIPGRPVF"
     gene            complement(10085..10282)
                     /locus_tag="PK264_07740"
     CDS             complement(10085..10282)
                     /locus_tag="PK264_07740"
                     /inference="COORDINATES: protein motif:HMM:NF026182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HRD75813.1"
                     /translation="MPFVNIRILEGHSQERKDEMARRITETITEVAKLPKEAVWVVFE
                     DVKATDWFVGDRSVHKIKGGK"
     gene            10588..11085
                     /locus_tag="PK264_07745"
     CDS             10588..11085
                     /locus_tag="PK264_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004107869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRD75814.1"
                     /translation="MAKKPKAAPTPAGPPAVPAKRVDRWLLKSEPDVFSWAMMKSRGA
                     TGEPWTGVRNYLARNNMRSMQIGDLAFFYHSNIGKEIVGVVRVIALAHPDPEDETGTW
                     ACVDVAAVADLPSPVSLQAIKDNPKLSGMALVTTSRLSVQPVTPAEWREVCRMGGLDA
                     RRLAE"
     gene            11337..12023
                     /locus_tag="PK264_07750"
     CDS             11337..12023
                     /locus_tag="PK264_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HRD75815.1"
                     /translation="MSSRSLIQACGAPAVASGQDDTQSALLTRRSLLLSVAAATLILP
                     AGTASAAAVAGAVSDLTGDAFAEAAGQRRPLATGANVFVADTVATGEEARVALKLGRA
                     TLVRLGAKARLKIDAHLIDVGGELDLQSGPMLFDRKSSTAARRETVVRTPYGLLAVRG
                     TRFWGGMSNGVFGVFVDEGRVDVSAAGRTVRLTRGLGTDIAAPGARPTPPKPWVAERI
                     EAALATVMAK"
     gene            12161..>12722
                     /locus_tag="PK264_07755"
     CDS             12161..>12722
                     /locus_tag="PK264_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD75816.1"
                     /translation="MIPNTSPSFNFDLGETTDQLRDTVRAFAQNEVSPIAAEIDRTDR
                     FPRELWPRMGELGLHGITVEEEHGGAGLGYLDHVVAVEEISRASAAVGLSYGAHSNLC
                     VNQIRRNGSEAQKRKFLPKLLSGEHVGSLAMSEAGAGSDVVSMQLKAEKRGDRYILNG
                     SKMWITNSPDAQLIVVYAKTDPTAGPR"
     assembly_gap    12723..12736
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12737..13336
                     /locus_tag="PK264_07760"
     CDS             <12737..13336
                     /locus_tag="PK264_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD75817.1"
                     /translation="FIVETSAKGFKVAQKLDKLGMRGSSTGELVFDNVEIPEENILGG
                     VGRGVNVLMSGLDYERAVLAAGPIGIMQAAMDIVLPYVHERKQFGQPIGSFQLIQGKL
                     ADMYTTMNACRAYVYAVARACDRGETTRRDAAGAILYASEKATHMTLDAIQILGGNGY
                     INDYPTGRLLRDAKLYEIGAGTSEIRRWLIGRELFEATA"
     gene            complement(13477..14232)
                     /locus_tag="PK264_07765"
     CDS             complement(13477..14232)
                     /locus_tag="PK264_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRD75818.1"
                     /translation="MSEGRTTAADGAKQRDRELRAFLDGTERAPSKLELIAAFEQFMR
                     RQRFHDYVCMRLRDLRRLDDDSILLCSRGDSWWRAYRASGVHHYNPILKVVQDRRTFA
                     WSDVLDNATMDKKERECMELAYRYGVRNALVVAVSEPEGDAQGVFSIGSPNIEFNVVV
                     REQLRLAIEAFYKSLAKFIRAEFQFTSQLTPREIEILRWVSEGKSDWQIGKILGISAK
                     TVNYHTENVKRKFDVTTRIQAVMRAVQSKTLSI"
     gene            14408..16096
                     /gene="murJ"
                     /locus_tag="PK264_07770"
     CDS             14408..16096
                     /gene="murJ"
                     /locus_tag="PK264_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRD75819.1"
                     /translation="MAAAAIVPNGPLPGGPPIAPIAHGACCGYASCMKLYRAFATVGA
                     LTMVSRVAGFVRDVLIASVLGTGVVAEAFFAALRFPNLFRRLFAEGAFNAAFVPMFAR
                     RLEQQGPDAARRLAEEAMAGLALVLTLLSALAMIAMPWFTLVNAPGFSEDPAKFDLTV
                     ALTRITFPYLACMSAVALLSGVLNSLKQFAAAAAAPILLNVVLISAILAAKLLDLGPS
                     AFTGQLLASGVALAGLAQVALVWMAARRRGFTILPRLPRLSLDMRELVRLGVPGLIAG
                     GVTQINIMVGGMIASFAAGAVSFLYYADRLYQLPLGVVGVAIGVVLLPEITARLAAGD
                     EASALDSQNRSLEFALLLTLPAAIGLALAAEPIVRLLYERGAFTASDTTATAWALAAY
                     AWGLPAFVLQKVLQPGFFARSDTRTPMRFALINLVANVALSLALFFGFQKLGLMPHVG
                     LAIATTLAGWLNTILLWTTLAKRGHFALDATARRIVPRLLVASALMAAALAVASHVAG
                     AWVVPGAGLAVKSAAVAAIVLTGITSFAVAAELLGAVRWRSIAGSLRRSRRGPS"
     gene            complement(16093..16536)
                     /locus_tag="PK264_07775"
     CDS             complement(16093..16536)
                     /locus_tag="PK264_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75820.1"
                     /translation="MATLAVSLQLADPARLMADPLADRLAEVGKAWAPHKLSVRPAVI
                     SATPILPEHAWLSRFEAVAKANMPSVGDTHMVPVVGGGEHAVRVVGIREIDAVKLGID
                     APAGGRRLVLISLQLPGDGDGRLVRIITETTGDSAPRSDAGKTPL"
     gene            17226..17759
                     /locus_tag="PK264_07780"
     CDS             17226..17759
                     /locus_tag="PK264_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD75821.1"
                     /translation="MTNLLQSLAERRDVSAFQTLYQTYGPRVKTYMMRHGADAASAED
                     LAQETLLQVWRKASLYAPDKGSVSTWIFAIARNLRIDRLRRELPLQALPEDHGETASD
                     DVPPDEQASTHERQRRVRAALAALPTELREVLTLAFVDGLSHGAIAERTGVPLGTIKS
                     RIRLGYQKIRTAIEDLA"
     gene            17756..>18001
                     /locus_tag="PK264_07785"
     CDS             17756..>18001
                     /locus_tag="PK264_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75822.1"
                     /translation="MTIRHHLDDATLIAFAAGTLPPPLALVAAAHLELCARCRREMSL
                     LDALGASFLDGLPESALSRPAPVVAARSAAVEPVEADV"
BASE COUNT         2955 a         6203 c         5572 g         3101 t
ORIGIN      
        1 aggtgctctt gtcgtcgcca gagaggaagc ggaacgtgcc ccacagccag tggtgcagat
       61 agtcggcttc ctgcagtcgg atgaactcgt cagaagcgat atgggtgccg cggaaaatcg
      121 aacagcggcc ggtcagcgtc agcacgcgat tggacagcgc atggctttgc atcgccatgc
      181 ggcgctgtgc aaagcgcatg ttgagccacg actgcatcca ccaggggccg cgcacgatga
      241 cgtgttcgtc cgtcgtcagc gcatgcagct tcggatcgca ggcgaagagc ggcgccgact
      301 tccgcacgag ccccgggtga tagacgaagt cgccgtccat gaagatgacg aggtcgtcct
      361 tggcgagccc cgcgcgcgac atggcgcgca ggatgagcgc gatggccacg cgcttgcccg
      421 gctggttctg gcggatgatg cgcaacgtga tgtcgaggtc ggaggcgacg agcttcaggt
      481 gccgggcgat cttctgctcg tcggcaagct cgctcgagcc gagccagagc gtcnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccccagc cgctctccca gagccgtgcg
      661 gcacgctccg ccattgccgg ataggtgacc ttctcgaaga ccttcgcgcg gatccagtgg
      721 ttgaaccacc agaggtagcg ccagataccg aggatgccga tcacatagat gaagtcctgg
      781 acctccgggt cccagatcgt attcggcacg tacgacatca cgacgaagca gaccgcgaag
      841 aacgcggcga tcttcatctg cacgagcggc gaccatttct cggtgaacga gagcttcacc
      901 gtcgggtcgc tttcgcggat cagctgctca tacatgggcg ggcgcctcac atcgcgcggc
      961 tgatcctgtt gcggatcgag gccatcagcg agttggtcga gcgctgcggg aaaatggcga
     1021 tcacgggcgt cccggagcga tagcgctcgg catccgcgac gcgggtcgga tcgtcgaagg
     1081 tgatcgtcac cttggcagag cggtcgacgg agccgcgcca gcgatagccg ggcgccgtcc
     1141 gcgcgttctg ctcggcgatg aaccccgtcg tgcggtcgat ccctgtgacg cggcccttga
     1201 gcgtctcgcc gacggccgcg atgaagagcg tcacctcgtc gccgagaccg accttcatca
     1261 cctcgtcctg cgtcaggaat gcaagcacgt gacgcttggc gcgttgctcg atcaccgcga
     1321 tcacgtcgcc gcggcgcaca tgcccgttgt cgacgtgcgg cagctccagc accgtgccgt
     1381 cgaacggagc ggtcacggcg agccgcgcgc ggtgattggc gagcgccagg aagcgctggt
     1441 ggaagagctg gatctcgtgc tcggcgagcc gcacctgcgc ctcgatcagc ccgacgtcgc
     1501 cgacaatgtt ggcgcccgta tagtgccgct tgccgatgtt ggcgccggca agctccactc
     1561 ggctcttgag ttcgacgcgc ttcccttcga tcacctggcg gagcgcgatc accgccttct
     1621 gcgcctcctc gaccttggtg tcggtcgcat agcccttctt gtgcagcgag gtgagccggc
     1681 cgagctgcgc ctcggcgagg gcgagctgcg actgcagccc gtcgatctcg atgcggatct
     1741 gctcggcgcc cttcaagtcg atcgcggcga aactctgcag ccnnnnnnnn nnnnnnnnnn
     1801 nnnnnnctcg agctcgtcgg cgtggcggcg cttcatgtag atgagcttcg ccttctgctc
     1861 cttgacggcg atctcggcga gctcgatctc gcgctcgagc tgattgtcga cgagcgtcac
     1921 gatggactcg cccgagcgca cggtatcgcc gggcttcagg ctgccggtct tgatccggcc
     1981 ttccgcctgc gatgcaaccg actcgacggg cgaggcgatc accgccgtct gcacctcgag
     2041 gcgatagaaa ttcgaataga ggagcagccc cgtgtagccg aacacaagga agccgagcac
     2101 agcgtagacc gccgacatca ccagcgcctt cgacgggcgc cggcgcacag gcaccgcctt
     2161 cggcgggctc gcatcgggct gcagggaggc cggcgtcacc ggcacgtcga tgcgctggat
     2221 ggtgtcctcg acatccacca tcgtgccgcg cacgagctcc tcgatgaagt ggctcatgag
     2281 ctcgcgctcg cgctcaccga gctcggtgaa gcggcagccg atcatgcgcg catcggcgtc
     2341 gaagcgcatc acctcgacct tggcatcgaa ggtgacgtcg aagccctgga agggaagcgt
     2401 cagctgcagt gcaagctcgc tgccgatcgt cggcagctcg ccctcgaccc catcgagccg
     2461 caacccgccg aggctccagt cggtcgcacg gtagcgccga ccgtcgatgt gtacgtacat
     2521 tggggcggtg acgcggtgat gacggcgctg gtcaggccgc tcccgcttga ttttcaccgc
     2581 catggtccga actcgttact agacgctact actcgttggg cccgagcacc cctatgccga
     2641 gccaccctcg cttatgacag gttgcagtta agattggatg tgcacgacga ggcgcggcgt
     2701 gccgccttgc agcggcacca ctcgttcgcg agagcaccgt gagaaccgtg tctacttgct
     2761 cgtcctcggc gcctgcccgc gcttcaggcg gcgtgcatcg ccgatcgcga gcagcttctc
     2821 acgatcctcc ggtcgcacct ggttgtagcg ctcacgccat tcaggaccgc ccttccaatc
     2881 gaagggggaa cgcacgaatg accgcgctcc agccgccgtc tcgagctggc cgagcgccag
     2941 ccgcacgatc gcgtcctgca tggtcggctg ccagggatgg ccgcacggat tgccgagcgg
     3001 aaaatcggag tagacgaacc gcggtacgcc gcagtgctcg acgatgtccc tagccgagcc
     3061 gatgatcacg gtcggaatgc cgttcgcttc aaggtatcgg gccgtcaaac tgacggactg
     3121 gtggcagacc ggtcaaagcg cggtcagcag cgcggcgtcg gccccgtcct cgcggatgcg
     3181 cttcaggatc tccggcgcgt catgctccag cgtcttcttg tggctgtagt cggtcggcac
     3241 accatgaaag cggggcgcga tgcacccgac aacgccctgc tctaccagtc ggccgaccgc
     3301 gtggatcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacggtcc caggcgaggt
     3361 cggtgtcgaa acccgcaggc ggcgcggcaa tgtcacccga ccagacctgc ttgcggccct
     3421 tggcatcgcg gttcgaacgg tcgggcggac cggccgtggt gacgagcgcc acgcgtgccg
     3481 ctgccagcgg cttcccgagc ttcgtgaacg gcacgtcgtc gaagtgtgcc cagacgtagt
     3541 cgttcgtgta gccgagtgcg cggtagtaga gacgcgtgcg ctccatgtac gggacggacc
     3601 tctcggtcga cggctctgac atatgttccc ccgggtgcgt tgtcagtccc actatgtcta
     3661 ccacgaaccc cgctcgcaac ccgcaacgtg ccgtggctgc cccgcgccgg acgcggggcg
     3721 ggccatactg tcccagccgg tgacagagcg ttgcccccct cccccgaatc gaggcactca
     3781 ggttgtacac ccacgaggag cgggggccct ccatgaatgt catgtcgacg ccgacggtga
     3841 agccaatggc accggatctc gcagaaatag ccgccagcac ggcagagctt tgcatcgcga
     3901 cgcgcgtgcg gcagttgagc cgcatcgtca cgcgcgtcta tgacgatgcg ctgcgtccac
     3961 tcggcatcac ggcgagccag tacacgctgc tcacgcagct cgccaatcgc gacggcatca
     4021 cggccgccga gatcggcgcc acgctcgaca tcgaaaagtc gacgctctcg cgcaacttga
     4081 agcgcctgct ggcgctcggt ctcatcaaca tggacccgcc ggctggccgc cgtggacgtg
     4141 gcctgcacct tacgccgcgc gggcaggccg tgatcaagga cgcctatccg atctggtccg
     4201 ccgcccagaa gcggtctaca gagatcatgg gcggcgaaag ccgggcgatg ctcgacacac
     4261 tcctcatgca ggccgaacgc ctaatccccg cctgaccgtc agccactatc gccgaaatca
     4321 gcgccgcatt cagcgattgc tgatcgcctc gatccaaggc accttcgctt cgcgcccaga
     4381 ttggagcgct tgaagcggag gcattgccgt gttcgatctc gagcttctga cagaggccgc
     4441 ctcactgctc gaggtcctgc gccagcgtac gctcaagatt gtgacggcgg agagctgcac
     4501 gggcggcctc atcgccggcc tgctcaccga gatcccgggc tcctccgacg tcgtcgagcg
     4561 cggtttcgtc gtctattcga acgacgccaa gatggaaagt ctcggagtac cgggccatct
     4621 cttcgccgac catggtgccg tcagccgtcc cgtcgccgag gccatggcgt caggcgccct
     4681 catgaattcc ctcgccgatg tcagtattgc tgtgaccggt gtcgccggac caggtggtgg
     4741 caccgcgctg aagcccgtcg gcctcgtgca tatcgccgcc gccacgctcg gtgggcagcc
     4801 gctccaccag gagtgccgct tcggcgacat cggtcgcagc gccattcgcc tggagacgat
     4861 caaggtcgca ctcgccctcg tgcgccgatt gaccatcgac atgcccctgg ccccgcccgc
     4921 cgacgcttga cgccggctgg cggtccctgc gacgaaagcg gcctcaagcc agacgctcga
     4981 tcgcgactgc cgtcgcctcg ccgccgccga tgcagagcga agcgaccccc ttcgtctcac
     5041 cgcgcttctt gagcgcattc atcagcgtca cgatgatgcg cgcgcccgag gcgccgaccg
     5101 gatgcccgag agcgcaggca ccgccatgca cattgacctt gtcgtgcggc agatcgagat
     5161 cgcgcatcgc cgccatggtc acgacggcga acgcctcgtt gatctcgtag agctgcacct
     5221 ccttcgccga ccagccggcc gccgccagca cctttttgat cgagccgatc ggcgccgtcg
     5281 tgaaccaggc cggtgcctgc gcatggctcg ccgtcgcgag gatgcgggca agcggctcga
     5341 gcttgcgccg cttggcctcg ctctcgcgca tgagcacgag tgccgcggcc ccgtccgaaa
     5401 tcgacgatga gttcgcgggc gtcaccgtgc cgccgtcgcg gaacgcgggc ttcagggtcg
     5461 ggatcttcgc cggatcggcc gtgaagggct gctcgtcgcg cttcatctcg ctcgtgccct
     5521 tgcgcgactt caccgtcacg ggagcaatct cggcatcgaa cgagccgtcc tcgttggcgg
     5581 ccttggcgcg gcgcaggctc tcgatggcgt aggcgtcctg cgcctcgcgc gtaaactggt
     5641 agtgctgcgc ggtatcctcc gcataggtgc ccatcaaccg gcccttgtcg taggcatcct
     5701 cgaggccatc gaggaacatg tgatcgtaga cctgggtgtg gccgaggcgc tggcccgagc
     5761 gcatcttcgg caggatgaac ggcgtgttcg tcatgctctc gaggccgccc gcgacgatca
     5821 cctcgcctgc cctgacgtgc agcgcttcga agcccatcat cacggtcttc atgcccgagc
     5881 cgcacatctt attgacagtc gtgcaaggca cgctgtcggg aaggcctgca tagcgcgccg
     5941 cctgccgcgc cggcgcctgt ccctggcccg ccggcagcac gcagcccatc agcacctcgg
     6001 cgatctcgtc gggcttcacg ccagcgcgcg caactgccga cttgatggcg accgccccga
     6061 gctccggggc aggcaggctc gatagcgccc cctggaagcc gccgatcggt gtgcgcgctc
     6121 cggaaacgat gacgatcggg tcggcggtct tcgacatgtc actctcctcc gtcaggttat
     6181 gcctcgatgc ggacacaggt tagcccctcg cggccatgct gcaaagcagc atgcacatgg
     6241 cgccattgtg ctcacaagga gcgttgccgg agctcgcttg tgtaccgcct catttcggtt
     6301 ccgctttggc cgcgtccgat cccgaatcag cttgctgttt aggtctcgtt agtcgaatca
     6361 tcgtcgtatc acccgcgggg ttgaacggcg gtcgcggggg aagccagaga tgctgatgag
     6421 cgtattcgag gacatcatcg aggcgaccga gcatccagag ctcgcgggaa agcgcgtgct
     6481 cgtcaccggt ctgacgatgg cactcggcgt cgatgtcgcg cgcgccttcg ccgagcaccg
     6541 cgcccgtctg gtgctgcaca tcgccgagga cggaccggag gccgacgcgc ttgcctcgta
     6601 tgtgtcctcg actgcgctcg atctcgagct ctactccggc gcgatcggcg acaaagacgc
     6661 ggcgaccaag ctcgcccagc gtgcctcgac cgcattcggc ccgctcgatg ttatcgtcaa
     6721 cctcgccgtg atcgcgacgc cacgcacggg cgatgcggcc accgaagccg agatcgagga
     6781 cgctgtcagc tcgggcttgc gcgcgccgct cgtcgtgacg cgcatcgccg ccaaccgcat
     6841 gcgtctcacc atgaccggcg ggctgatcct gaacgtcgcg atcgtcaccg gtcccgacag
     6901 caaggcgcgc cgcgccgtcg ccgcggtgac gcgcacgacg ctcgccgcca tgacgcgcgc
     6961 tgaagcggcg tcctgtgccg ccgacggcat ccgcatcaat gcaatcgcac cccgctcggg
     7021 cctcttcgag cacgagcgcc atctccagaa cgagccgcag atcgccaacg tcgcgctcta
     7081 cctcgcgagc aagaagggcc gcgagcacac cggtctcgtg ttcgaggcga acgggccgca
     7141 gtgccgctga gccggcacgc gaggcaatgt ctaccagatg cactccctca gatgctcctg
     7201 ctcgtcgagc tggtcgatct cgtagcccgt aaaatcggcg cgcgtgacga cccctaggac
     7261 cttgccttcc gccaccacag ggagatgccg gaagccgccg tcgcacatgg ctcgtaacgc
     7321 ttcgatcgcg cgggcgtccg aggcgatcgt caccggcttg cccgtcatgg ccttgctgac
     7381 ggcagtcgcc tccggcgcga tgcccgaccg gatcgccttc actgcatcac gtccggtaaa
     7441 tatgcccttg agatgcccgc acgcgtccgt cacgagcgcg aagcctcgac tttccttcag
     7501 catggtctga cagacgtgct tcaccgtctc gtcactcttg gcgatgagcg gtgtcttgcc
     7561 ttcgatgatg tcgccgacgc ggcggtcggc cgagttgccg agcccgccgc cgagttcgcg
     7621 ccagaggaac gcttcgaact ccatgccctt gaagtcgccc ttcgaaacga cgccgacgat
     7681 ctcgtgatcg tccgtcaccg ggacatgccg gaagccgccc gctgccatcg ccttcagcgc
     7741 ctcgattgcc gtgcagtccg ggcgcaccga cactggacct tccgtcatcg ccttggcgag
     7801 cttgacctcg cccggcgccc tgcccttcgc gatcagccgg acggcgtccc ttccggtgaa
     7861 gatgccggtc aaatggtgct ccttgccgac caccagcacc gagcccgcgc cgtgctcgca
     7921 catcagggcg caggcgcgtc ccgcggtctc gctttcggca aggacgagcg gcttctgatc
     7981 tttcacgatg taggcgagct tgcgattggt catgaccgtc acgctccttg aaatcggtgg
     8041 atggtctcgc tgccccagcg tggccctctc aaactcaatt cgccccgtcg agactattga
     8101 tgtaggcgac gatgcggtcg atgtgatcgt ccgccagcac gaatttcggc atctcgtcgt
     8161 gcggggccgc gatcgcgcgc gtcagcggct ctcgcaaggc ctccatggga tggcgcgcgt
     8221 gaagcgtgcg gaaggcgggt gcgccggcct tcgggctcgc gccttcgcga ccgatggcat
     8281 ggcagctctt gcaatgcgtc tcgacgagcg ccgccccttc gccgacccgc ttcgccgcct
     8341 cctcggcgag cggcgcacta ctgcgcagcc gcgcgagccc gtccctggcg ccgatatggc
     8401 tcggatcgaa caaaagtgcg cgttgcaggt cctggatcgc ctcggcgcgc tttccctgcg
     8461 tttcgtaggc atagccgcga ttggcatagg cgaccgcata gaccgggttc aggaggatcg
     8521 ccgcagagaa gtcctcgatc gcacgcggaa catctccggc ctttcgataa gcgattgttg
     8581 tgggcgctcg ggctcttcgg attgagcttc agcgcctccg agaagtccgc gatcgcgggc
     8641 gccgctgctc ccttcacgag gttggcatag ccccggccgg tgaacccggc tgccgacttg
     8701 ggattgatgc gaatgacggc gctgtagtcg gcaatggcgc cgtcgtggtc gccgcgccgc
     8761 acccgcacga ggccacgaaa gacgtaggcc tgaatgtcag cgccgccgag ccggatcgcc
     8821 tcgtcgaggt cggcgatcgc gagatcaagg actccggcct ccgtgcgctg gcggccacga
     8881 ttgcgatagg caatggcctt ctgctcgacg gagtatccgg cccccgcgat cacgtcgctg
     8941 caggccgcga gcttctgttc ggggcgggcc ggtccacggc aagtctcgaa cgcatccgcg
     9001 cgggcgccac ttgcggcacc ccacacgagg gcagcggcaa gcaccagtgc attgagtgac
     9061 gtcatcagat cgcatcgtga catgggatcc tccaaggacc ctgtcatcgt tacgtcatga
     9121 tcgcccaccg gccgttgaca ggcatcaact ggacgccgga ccctcccggt tcggcgcctc
     9181 attgatgatc agaacaccgg ccggcccggg atgcgggtgc ggatgcgata gagcgacgtc
     9241 gaggccgtga tgtagagcga gcgatagtcg ccgtcgcccc agcagaagtt ggcggtgtac
     9301 tccggcacgc ggatcacgcc gagcgcgcgg ccgtccggat cgaacacgtg gatgccgccc
     9361 ggtccacagc tgtagacgtt gccgatggca tcgattttca tgccatcggg agcgccagct
     9421 ccttcgcccc gcgtctccgc ccacacgcgc ccgttggcga gcgtgccatc gtcgcgaaca
     9481 tcgaacacgc ggatgtgctg gcgctccgta tcgctgacga acaggcgctg ctcgtcgagc
     9541 gagaagcaga gcccgttcgg ctgcttgaag tcgtcggcga ggcagcgcac cgcaccggtc
     9601 ccgggatcga tccggtagac accctggaag ctcagcgcct gcggccgctt caagccgaac
     9661 ccgtccatgc gcccataggt cggatcggtg aagtagatcg catcgtcgtt gcggacgatg
     9721 atgtcgttcg ggctattgag ctcggtgtcg ccgttgtggc tcgcgagcgt cgtcgtgctc
     9781 ccgtcggcct cgaggcgcgt gaccctgctc gaggcatgct cgcatatcag catgcgcccc
     9841 gacttgtcgt agacgaggcc attcgacttg tgcgtcggct ggcgaaacgt cctgacgccg
     9901 cccttggcgt cccagcgccg catgatgtcg cccggcatgt ccgagaacgt gagatgtccc
     9961 tcgaccggat gccagagcgg cccttccgtg aacttgaagc cggtgccgag cttctcgaac
    10021 gatacatctg agccgacgac ctccgtaagg cgccggtccc gaacctcgag attgcccatg
    10081 accgctactt cccgcccttg atcttgtgga cgctcctgtc accgacgaac cagtcggtgg
    10141 ccttcacgtc ctcgaacacg acccacacgg cctctttcgg cagcttcgcc acctcggtga
    10201 tcgtctccgt gatgcggcgc gccatctcgt ccttgcgctc ctggctgtgg ccctcgagaa
    10261 tgcggatatt gacgaacggc atggggtcct cctgcttggc ttgtgcggct ggctgctgcc
    10321 ttatcgccct cagatcggcc ctcgcccgaa ccggagctta tcattgcaat ctgcccgatg
    10381 ctccacgcag cggcaaccag caggttgatc ggacacccct cgcgaccatc tgacggccga
    10441 cccgtgggta cacgggccat gtcgaccacg atcatcgacg gcagcgtcag ctgcaggctt
    10501 gtgttatcgg tggcacccgc caagcgcacg accccaggca catccaggtg tgcaggacga
    10561 cacgcaacca cgaggggcaa ccactaaatg gcgaagaagc cgaaggccgc gccgacgcca
    10621 gccggtcccc ccgcggtgcc ggccaagcgg gtcgaccgct ggctccttaa atccgagccc
    10681 gacgtcttct cctgggcgat gatgaaatcc cgcggggcca ccggcgaacc gtggacaggg
    10741 gtgcgcaact atctcgcccg caacaacatg cgctcgatgc agatcggcga tctcgccttc
    10801 ttctatcact ccaatatcgg caaggagatc gtcggggtcg tacgcgtcat cgcgctcgcc
    10861 catcccgacc ccgaggacga gacaggcacg tgggcctgcg tcgacgtcgc agccgtcgct
    10921 gatctgcctt cgcccgtttc gctgcaggcg atcaaggaca atccgaagct ttcaggcatg
    10981 gcgctcgtca ccacctcgcg gctttcggtg caacccgtga cgcctgccga gtggcgcgag
    11041 gtctgccgca tgggcgggct cgatgcccga cggcttgccg aatgatggtc gccagctgag
    11101 ggcagcgtcc acatcggcgt gttctccggc ggtcatccgt tccaaggtgc ggaagcggcg
    11161 cgcctgctca tcatgtccag catggcgcat tcccacgtag aaggaggcgt ggcaccctga
    11221 ccttgcgtgt cggtccggac cggatcgccg actgagtgca gtgatccaca gcactgatca
    11281 atcgccggac atctggcaga gatgatataa acattgttca tttggagcgg acgaccatga
    11341 gttccagatc cctcatccag gcctgcggtg cacctgcggt ggcgtccggc caggacgata
    11401 cgcagtcggc gctgctgacg cggcgaagcc tcctcctcag cgtcgcggca gcgacgctga
    11461 tcctgccggc cggcacggcc tcggccgccg ccgttgccgg cgccgtcagc gacctcaccg
    11521 gcgacgcctt cgctgaggcc gcaggacagc gccgtcccct cgcgaccggt gccaacgtgt
    11581 tcgtcgccga cacggtcgcg accggagagg aagcgcgtgt tgcgctgaag ctcggtcgcg
    11641 cgactctcgt ccggcttggc gccaaggcgc gcctcaagat cgacgcccac ctgatcgacg
    11701 tcggcggcga gctcgacttg cagtccggcc cgatgctgtt cgaccggaaa tcgagcacgg
    11761 ccgctcgccg cgagaccgtg gtgcggacgc cctacggcct tctcgccgtc cgcggcaccc
    11821 gcttctgggg cggtatgagc aacggcgtct tcggcgtatt cgtcgatgaa ggccgcgtcg
    11881 acgtcagcgc cgccggacgc accgtgcgcc tcacccgcgg tctcggcact gacatcgcgg
    11941 cacccggcgc ccgcccaacg ccgccgaagc cgtgggtggc cgagcgcatc gaggccgcgt
    12001 tggcaacggt aatggcgaag tagctgcagc gaccgacgag gctcagcgca gctatgcggc
    12061 cggtcgccgc acttcgctct cgccagcctt gcgacgactg cgctatgcgt taactgcagg
    12121 cgcctcgcat gacgcgccgt cctctgcagg gccctcaacg atgatcccca atacgtcccc
    12181 gtccttcaac ttcgacctcg gcgaaaccac cgaccagctc cgcgacacgg tgcgcgcctt
    12241 tgcccagaac gaagtctcgc cgatcgccgc cgaaatcgac cggacggatc gcttcccgcg
    12301 ggagttgtgg ccacgcatgg gcgaactcgg cctgcacggc atcacggtcg aggaggagca
    12361 tggcggcgcg ggcctcggct atctcgacca tgtcgtcgcc gtcgaggaga tctcgcgcgc
    12421 ctcggccgcc gtcggcctct cctacggggc tcactcgaac ctctgcgtca accagatccg
    12481 ccgcaacggc agcgaggcgc agaagcgcaa attccttccg aagctgctct cgggcgagca
    12541 tgtcggctcc cttgccatga gcgaggccgg tgctggctcc gacgtcgtct ccatgcagct
    12601 caaagccgag aagaggggcg atcgctacat cctcaacggc tcgaagatgt ggatcaccaa
    12661 cagcccggac gcccagctca ttgtcgtcta cgccaagacc gatccgacgg ccggcccgcg
    12721 cgnnnnnnnn nnnnnnttca tcgtcgagac cagcgccaag ggcttcaagg tcgcccagaa
    12781 gctcgacaaa ctcggcatgc gcggctcctc cacgggcgaa ctcgtcttcg acaacgtcga
    12841 gatccccgag gagaacatcc tcggcggcgt cgggcgcggg gtgaacgtgc tgatgtcagg
    12901 cctcgactat gagcgcgcgg tgttggccgc cggccccatc ggcatcatgc aggccgccat
    12961 ggacatagtt ctcccctacg tccacgagcg caagcagttc ggccagccga tcgggtcgtt
    13021 ccagctgatc cagggcaagc tcgcagacat gtacaccacg atgaatgcct gccgggctta
    13081 cgtctacgcc gtcgcccgtg cctgcgaccg cggtgagacc acccgccgcg atgcggccgg
    13141 agccatcctc tatgcctctg agaaggcgac acacatgacg ctggacgcga tccagatcct
    13201 cggcggcaac ggctacatca acgattatcc gacgggacgc ctgctgcgcg atgcaaagct
    13261 ctacgagatc ggcgccggca cgagcgagat ccgccgctgg ctgatcggac gggaactctt
    13321 cgaggcgacg gcctgagcga ctgcctgaaa gggcaagcca ggcgccacag caaagcatca
    13381 acgacatgac cctgacgtcg aaaagctgat cagactaaag aacggctcgc attggcgacg
    13441 accccaacct gaaagtcgag gagcccgaca aatatatcag atactcaacg tcttcgattg
    13501 cacggcacgc atcacggcct gaatgcgcgt cgtcacgtcg aatttccgct ttacgttttc
    13561 cgtgtgataa ttgacggtct tggcgctgat cccgagaatt ttcccgatct gccaatccga
    13621 cttcccttcc gacacccaac gcaggatctc gatctcgcgc ggcgtcagct ggctggtgaa
    13681 ctgaaactct gcacgaatga attttgctaa ggatttgtaa aaggcctcga tggccaacct
    13741 caattgctca cggacaacga cattgaactc gatattcggt gaaccgatac tgaatacacc
    13801 ctgggcatcg ccctcgggct cagacacggc gacaaccagc gcattacgaa ctccgtaccg
    13861 gtaggctaac tccatgcatt cgcgctcctt tttgtccatc gtggcgttgt cgaggacatc
    13921 ggaccaggcg aacgtgcgcc ggtcctgcac caccttcagt atgggattgt agtgatgcac
    13981 gccggaagcc cgataggcgc gccaccatga atcaccccgg ctgcagagca gaattgaatc
    14041 gtcatccaac cggcgcagat cacgaagtcg catgcacacg taatcgtgaa atctctgacg
    14101 ccgcatgaat tgctcgaacg cagcgatcaa ttccagtttc gatggcgcac gctcggtccc
    14161 gtccagaaag gcgcgcaact ccctgtcgcg ctgctttgcg ccatccgcgg ctgtggttcg
    14221 tccctcggac atcacaactc cgccttccag aaaagaacaa cctgcccgga aatgatacgg
    14281 gcagccacaa gtttagcaca actccgagcg gaggcaagtt tcgcgccgat gctgcgccag
    14341 caactgccac aacggcctgg atacaatcgt attcccggtt tttttctcgc gcccgcccga
    14401 gtagcgcttg gcagccgctg cgattgtccc aaacggccca ttgccgggcg gtccgcccat
    14461 cgcgcccatt gcgcacggcg cctgctgcgg ctacgcctcg tgcatgaaac tttaccgggc
    14521 attcgccacc gtcggcgcgc tgacgatggt gagccgagtc gcgggcttcg tgcgcgacgt
    14581 gctcatcgcc tccgtgctcg gcaccggcgt ggtcgccgag gcgttcttcg ccgccctccg
    14641 cttcccgaac ctctttcgcc gcctgttcgc cgagggtgcc ttcaacgccg ccttcgtgcc
    14701 gatgttcgcc cgacgcctcg agcagcaggg ccccgatgcg gcccgccggc tcgccgagga
    14761 ggccatggcc ggcctcgcgc tcgtgctgac gctgctctca gcgctcgcca tgatcgcaat
    14821 gccgtggttc acgctcgtca acgcgccggg cttcagcgag gacccggcga agttcgacct
    14881 cacggtcgcg ctgacgcgca tcactttccc ctatctcgcc tgcatgtccg ccgtcgccct
    14941 cctctcaggc gtgctgaact cgctcaagca attcgcggcg gcggcggcgg ccccgatcct
    15001 cctcaacgtc gtgctgatct cggcaatcct tgccgcaaag ctgctcgacc tcgggccgag
    15061 cgccttcaca ggccagctcc tcgcctcggg cgtggcgctt gccggcctcg cgcaggtggc
    15121 acttgtctgg atggcggctc gccgtcgcgg atttacgatc ctcccccggc tgccccgcct
    15181 ctctctcgac atgcgcgagc tcgtacggct cggcgttccg ggcctgatcg ccggcggcgt
    15241 gacgcagatc aacatcatgg tcggcggcat gatcgcctcg ttcgcagccg gcgccgtctc
    15301 cttcctctac tacgccgatc gcctctatca attgccgctc ggggtggtcg gcgttgccat
    15361 cggcgtcgtg ctgctgcccg agatcacggc gcgccttgcc gccggtgacg aggcctcggc
    15421 actcgacagc cagaaccgaa gcctcgaatt cgcactgctg ctgaccctgc cggcagcaat
    15481 cggtcttgcc ctcgctgccg agccgatcgt gcgcctcctc tacgaacgcg gcgcgttcac
    15541 ggcgagcgac acgacggcaa cggcctgggc gctcgctgcc tatgcctggg ggttgccggc
    15601 cttcgtcctc cagaaggtgc tgcagccggg cttcttcgcg aggtcggaca cgcggacgcc
    15661 gatgcgcttt gcgctcatca atctcgtggc caacgtcgcg ctctcgctgg cgctcttctt
    15721 cggcttccaa aagctcggcc tgatgcctca cgtcggactc gcgatcgcaa cgacgctcgc
    15781 cggctggctc aatacgatac tgctctggac gacactcgcg aagcgcggcc attttgctct
    15841 cgatgcgaca gcgagacgta tcgtgcctcg cctgctcgtg gcatcagcac tgatggccgc
    15901 cgccctcgcg gtggcgagcc atgtcgcggg tgcgtgggta gttcccggtg cgggcctcgc
    15961 cgtcaaatct gcggcggttg cggcgatcgt tctgaccggc attacctctt tcgcggtcgc
    16021 ggccgagttg ctcggcgctg tccgctggcg ttcgatcgct ggctctctca ggcggagccg
    16081 cagaggcccc agctagagcg gcgtcttgcc agcgtcagac ctcggtgcgc tgtcccccgt
    16141 cgtctcggtg atgatgcgca cgaggcggcc gtcgccatcg cccgggagct gcagcgagat
    16201 gaggacgagg cggcgaccgc cggccggagc gtcgatgccg agcttcacgg cgtcgatctc
    16261 gcggatgcca accacacgaa ccgcgtgctc gccgccgccg accactggca ccatgtgcgt
    16321 atcaccgacg ctcggcatat tggcctttgc cacggcctcg aagcggctga gccacgcgtg
    16381 ctccggtagg atcggcgtcg ccgatatgac agccggccgc accgagagct tgtgcggcgc
    16441 ccacgcctta ccgacctctg caagcctgtc ggccaacgga tccgccatga gacgcgcagg
    16501 atcggcgagt tgcaggctca ccgccaaggt cgccacggct cccgcggccg ccagaagccc
    16561 tacaccgatg gtcccgagcc gaccgcgcga cgaagtgtaa agctcagatg atcgattgcc
    16621 ccgcatcctg tcctcaacgg tcattgatgc cctcagtcct gctcgagtcg acggccagta
    16681 cggcaagtat gtaggaagcg acaggttgga tttgcagccc cattgtggcc ggaaattgct
    16741 ctgaccaggc gacggcggtc agtgaccgaa ccttgtgcag tgcgctccgg ggcaccggcg
    16801 acacgccact acccccgaca tccatgtggt cgaccgatgc gtattggctg agaaaccacc
    16861 ctttggacga cacggcccag cgacagagga gtgactgagg agtgaccgcg aaggggcgac
    16921 ggcaggagga gcctccctct cctcctgccg cccggctccg gcctgatgcc caggtcgtga
    16981 gcgcggatca aaggtcgtgt cggcgctcgg cgggggacga ggggccggga ccttctccag
    17041 aggtgcggcc tggccggggg gaccagggat cgcaaccctc tgagccgaag ttagtgcaat
    17101 ggccctgagc ggaggctgaa cgcttcatgc agcactcatt caccgagcgt cgcgcggggc
    17161 tggccgatgc gtcaaagccg gcctacgttg agcccgccac gatcgaaccc tcagggccgc
    17221 gtttcatgac caatctcttg cagtcgctgg cggaacgccg ggacgtctcc gccttccaga
    17281 ccctctacca gacctacggc ccccgcgtga aaacctacat gatgcggcac ggcgcggatg
    17341 cggcctccgc cgaggacctt gcacaggaga cgctcctgca agtgtggcgc aaggcgtccc
    17401 tctatgcacc cgacaagggc agcgtctcga cctggatctt cgcgatcgcc cgcaacctcc
    17461 gcatcgaccg cctgcgccgc gagttgccgc tgcaggcgct tcccgaggac catggcgaga
    17521 ccgcttctga cgacgtgcca ccggacgagc aggcctcgac ccacgagcgc cagcggcgcg
    17581 tgcgagccgc gctcgcagcg ttaccgacgg agctgcgcga ggtactgacc ctcgccttcg
    17641 tcgacggcct ctcgcacggc gccatcgccg agcggaccgg cgtgccgctc ggcaccatca
    17701 agtcgcgcat ccgcctcggc taccagaaaa tccgcaccgc catcgaggac ctcgcatgac
    17761 catccgccac cacctcgatg acgcgacgct gatcgccttc gcggccggca cgctgccccc
    17821 gccacttgcg ctggttgccg ccgcccacct cgagctgtgc gcacgctgcc gccgcgagat
    17881 gtcgcttctc gatgcactcg gcgcgagctt tctcgacggc ctgcccgaga gcgcactctc
    17941 acgcccggcg ccggtcgttg ccgcccggtc tgcagcggtc gagcccgtcg aggccgacgt
    18001 c
//
LOCUS       DAORNH010000055        17937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2390_length_17937_cov_8.828878, whole genome shotgun sequence.
ACCESSION   DAORNH010000055 DAORNH010000000
VERSION     DAORNH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2390_length_17937_cov_8.828878"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="trbL"
                     /locus_tag="PK264_07790"
     CDS             complement(<1..858)
                     /gene="trbL"
                     /locus_tag="PK264_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HRD75823.1"
                     /translation="MGGTGVIDHFLEVFTRYIDSGFGLLSGEVAFIATTLIVIDVTLA
                     ALFWSWGADDDIMARLIKKTLFVGVFAYIIGNWNNLARIVFESFAGLGLKASGTGFST
                     ADLLRPGKVAQTGLDAGRPLLDSISDLMGYWSFFENFIQIACMFLAWALVLLAFFILA
                     IQLFVTLIEFKLTTLAGFVLIPFGLFGKSAFMAERVLGNVISSGIKVLVLAVIIGIGS
                     TLFSEFTAGFGGTTPTIDEAMAIVLAALSLLGLGIFGPGIANGLVSGGPQLGAGSAVG
                     AGLAAGGTVI"
     gene            complement(863..1243)
                     /gene="trbK-alt"
                     /locus_tag="PK264_07795"
     CDS             complement(863..1243)
                     /gene="trbK-alt"
                     /locus_tag="PK264_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009292 - horizontal gene transfer
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative entry exclusion protein TrbK-alt"
                     /protein_id="HRD75824.1"
                     /translation="MDGKLLARVGAVVFVALAITATAIEMTRKEEEPSSPVSGPMAAA
                     PADPLRAELMRCQGLGEAGPRDPACLRAWAENRERFLAPGARPMGRVPDPVAVPRPQS
                     ETSIEDPAGDPVRKESAPVPEEEQ"
     gene            complement(1254..2009)
                     /gene="trbJ"
                     /locus_tag="PK264_07800"
     CDS             complement(1254..2009)
                     /gene="trbJ"
                     /locus_tag="PK264_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HRD75825.1"
                     /translation="MKYRRSRAAIAAAFLSLTATVSPVFVTPAHAIIVYDPTNYAQNV
                     LQAARALEQITNQITSLQNEAQMLINQARNLASLPYSSLQQLQQSVQRTQQLLAQAQN
                     IAFDVQQIDRAFQTTYGNASLSASDQQLIADARTRWQNTVGGLQDAMRVQATVVGNID
                     TNRAEMSALIGQSQGATGALQATQAGNQLLALQAQQLADLTAVVAANGRAEALSQAER
                     AAAAEQGREQRRRFLTPGSGYQPGNAKMFYSGN"
     gene            complement(2006..4444)
                     /gene="trbE"
                     /locus_tag="PK264_07805"
     CDS             complement(2006..4444)
                     /gene="trbE"
                     /locus_tag="PK264_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HRD75826.1"
                     /translation="MMSLAEYRRTSTRLADFLPWAALVGEGVVLNKDGSFQRTARFRG
                     PDLDSAVPAELVAVAGRLNNAFRRLGSGWAIFVEAQRHGVAAYPSSIFPDAASALVDA
                     ERKAEFEEEASHFESSYFLTFTYLPPAEDAARAESWLYEGKSDKRVDPHEALRGFIDR
                     TNRVLQLVESFMPECGWLDDAETLTYLHSTVSAKRHRVRVPETPMYLDALLADQPLTG
                     GLEPRLGDQHLRILTIVGFPSATTPGLLDELNRLAFAYRWSTRAILLDKTDATRLLTK
                     IRRQWFAKRKSIAAILKEVMTNEASVLVDTDAANKAADADLALQELGADYAGQAYVTA
                     TITVWDADPRIAAEKLRLVEKVVQGRDFTAMPETINAVDAWLGSLPGHVYANVRQPPI
                     STLNLAHMIPLSAVWAGPERDEHFGSPPLLYGKTEGSTPFRFSLHVGDVGHTLVVGPT
                     GAGKSVLLALMALQFRRYPKSQVFAFDFGGSIRAAALACGGDWHDLGGGLTDGADHSV
                     SLQPLNGIHDVPERAWAADWIVAILVREGVAITPEVKEHVWTALTSLATAPVPERTIT
                     GLAVLLQSNDLKQALRPYCVGGAYGRLLDAESEHLGSAAVQAFETEGLIGTGAAAAVL
                     AYLFHRIEDRLDGSPTLLIVDEGWLALDDEGFAGQLREWLKTLRKKNASVVFATQSLS
                     DIDGSAIAPAIIESCQTRLLLPNERAIEPQITAIYRRFGLNDRQIEILARAMPKRDYY
                     CQSRRGNRLFELGLSDVALALCAASSKTDQAAIDRIVAEHGRDGFLGAWLRHRGVDWA
                     ADLIPDLTNLETLP"
     gene            complement(4456..4737)
                     /locus_tag="PK264_07810"
     CDS             complement(4456..4737)
                     /locus_tag="PK264_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HRD75827.1"
                     /translation="MTGVADSAGEVPGFTVPVHRALTEHILLGGAPRSIAILNGTLAA
                     ALGLGLRLWLVGLGLWAIGHLAAVWAARRDPQFVDVVRRHLRIPGHLSV"
     gene            complement(4737..5069)
                     /locus_tag="PK264_07815"
     CDS             complement(4737..5069)
                     /locus_tag="PK264_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HRD75828.1"
                     /translation="MMRHCPRLSRHISAAATAAFFSLALAPAAHASGSSMPWEAPLQS
                     ILESIEGPVAKIIAVIIIIVTGLTLAFGDTSGGFRRLIQIVFGLSIAFAASSFFLSFF
                     SFGGGALV"
     gene            complement(5066..6049)
                     /gene="trbB"
                     /locus_tag="PK264_07820"
     CDS             complement(5066..6049)
                     /gene="trbB"
                     /locus_tag="PK264_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HRD75829.1"
                     /translation="MAATHQQSEAILRGARMLRTALGPSIARFLEDPAIVEVMLNPDG
                     RLWIDRLSEGLADTGERLSPADGERIVRLVAHHVGAEVHAGRPRVSAELPGTGERFEG
                     LLPPVVAAPAFAIRKPAVAVFTLQDYVATTIMSADQAAILRQAVAERRNILVAGGTST
                     GKTTLTNALLAEVAKTEDRVVLIEDTRELQCAAPNLVAMRTKDGVATLSELVRSSLRL
                     RPDRIPIGEVRGAEALDLLKAWGTGHPGGIGTIHAGTALGALRRLEQLIQEAVVTVPR
                     ALIAETIDLVAVLSGRGSTRRLAELARVEGLTAEGDYRITPATHPLTGDPS"
     gene            complement(6297..6842)
                     /locus_tag="PK264_07825"
     CDS             complement(6297..6842)
                     /locus_tag="PK264_07825"
                     /inference="COORDINATES: protein motif:HMM:NF039512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HRD75830.1"
                     /translation="MGSDDWRDSSDQNIFEMCVTNGGPGFWIRRTTWGATCARVVGVG
                     AMTKPGPYFGNPSVLMDVYSLAGELRENLAQVSVPGTYKTWRRIPAPSWAEHANLRSL
                     DDPAISAALQSLDRKRHKAPREEQKVEERVLLSVPFSGKEEAKKLGARWSPAERLWWI
                     PASNKQALAQARTLGFLPDSN"
     gene            complement(7007..7453)
                     /locus_tag="PK264_07830"
     CDS             complement(7007..7453)
                     /locus_tag="PK264_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HRD75831.1"
                     /translation="MRDRMNVYFPPEMLRQIADLADRKKLSRSAIVEAAVASFLSPDG
                     ADKREAAFTRRLDRLSRQVQRLERDLGVTAETLALFVRFWLTVTPPLPNDGQGAAQIK
                     GRERYEGFIQTLGRRLQKGQSFLKEIPDDVAGGTSAPPTEAHEDDR"
     gene            complement(7461..9449)
                     /locus_tag="PK264_07835"
     CDS             complement(7461..9449)
                     /locus_tag="PK264_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HRD75832.1"
                     /translation="MSATKILWGQITTVFLIVLFTTWAATQWTAWRLGFQPGLGQPWF
                     ELAGWPIYYPPAFFWWWYFYDAYAPPIFVEGAYIAASGGFISIAVAILMSVWRAREAK
                     NVETYGSARWAELREVKAAGLLGPDGVVLGKLGDAYLRHDGPEHILCFAPTRSGKGVG
                     LVVPSLLTWPGSAIVHDIKGENWQLTAGFRAKHGRVLLFDPTNPKSAAYNPLLEVRRG
                     EWEVRDVQNVADVLVDPEGSLDKRNHWEKTSHSLLVGAILHVLYAGEDKTLAGVAAFL
                     SDPRRPIESTLKAMMTTPHLGKAGPHPVVASTARELLNKSDNERSGVLSTAMSFLGLY
                     RDPVVAEVTRRCDWRIADLIADPKPATLYLVVPPSDISRTKPLIRLVLNQIGRRLTED
                     LHAKDRRHRVLMMLDEFPALGRLDFFESALAFMAGYGLKSFLIAQSLNQIEKAYGPNN
                     SILDNCHVRVSFATNDERTAKRVSDALGTATEMKAMRNYAGHRLSPWLGHLMVSRSET
                     ARPLLTPGEVMQLPPTDEIVMAAGVHPIRAKKARYYEDRRFTERVLTSPDPAKCGRST
                     RKDGWSDLKPQRPDTVLLAEIEKAEDAANSGLRREPELPDHVAIVKETTEQAPAEEFA
                     IVLDDAPEDAARQRQILRQQMRGVARQVALDPNDGIDM"
     gene            complement(9477..11240)
                     /locus_tag="PK264_07840"
     CDS             complement(9477..11240)
                     /locus_tag="PK264_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HRD75833.1"
                     /translation="MSEGDSDFRIRPGRIRSTRAPKPKRFINQVLRAAKRAGHVSAHG
                     APGRRASSYGRSTFGRGRVSFSRARLFSPSRRVVVKARIARHQGRAFRSAPLAAHLSY
                     LKRDGVTRDGEKAVMFDAGSDSADDAGFAARAKDDRHHFRFIISPEDAGDMTDLRAFT
                     RDLASQMEADLGTRLDWVAVDHWNTDNPHVHLLVRGVDDKGADLVISRDYISRGLRSR
                     AEDLVSMELGPKPEHEIRHALEREITAERWTRLDVEIRIAADETGYIDLRPEQRGASD
                     PQTRRLMIGRLQHLEKMGLATAASPGEWMVGLDAERHLRDLGMRGDIIKTMHRAFAER
                     GQERGVPDYVIEGGAAQAPIIGRLVDRGLHDELTGEAYAVIDGTDGRAHHVRFRGIEA
                     FEHAPPIGGIVEVRCFGGADERRPTLVLANRSDLDLNRQVTAPGATWLDHRLVEREPM
                     PLSMGGFGREVRDAMQARAEHLAGEGLARRQGERIILRRDLLNTLRRRELDAVGAKLS
                     AETGLAHMKAAAGEHVAGVYRQRLTLSSGRFAMIDNGLGFQLVPWSREIERKLGQHIA
                     GVAKDGGGIEWSLGRKRGLGL"
     gene            complement(11488..12249)
                     /locus_tag="PK264_07845"
     CDS             complement(11488..12249)
                     /locus_tag="PK264_07845"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRD75834.1"
                     /translation="MSAPRRQFAAGRSLPARHLTLLLLSGLICAGATPLVPPAQAGPP
                     EAPRDVSPYAGFVAEAARRFDIPERWIWAVMRVESRGRVRAVSPKGAMGLMQIMPDSW
                     AELRARYGLGVDPYDPRDNILAGAAYLREMHDRYGAPGFLAAYNAGPGRYDDYVASGR
                     PLPAETVAYVAALAPLVGSEPLAHVVVAAANPLEWTRAPLFVAHSYSGGAADPALNER
                     QSSSDRAATPEDSDDAAEHPTGGLFVARSNGGGPR"
     gene            complement(12253..12591)
                     /locus_tag="PK264_07850"
     CDS             complement(12253..12591)
                     /locus_tag="PK264_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HRD75835.1"
                     /translation="MALIGQFSRTRNGYAGRVRTLGLDAELVLVPAEHSDAENAPDYR
                     IHLGSDADGPEIGAGWKRTGEKAGDYVSLQIDDPTFGQPVRANLFQSADDKSAWGLHW
                     NRPPKRGERD"
     gene            complement(12637..13182)
                     /locus_tag="PK264_07855"
     CDS             complement(12637..13182)
                     /locus_tag="PK264_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRD75836.1"
                     /translation="MTRFGYVVTTSVAVLLIGALSLVHVAPRLVWNASASAPVGLYTI
                     GASTPLEVRDLVAVDAPEPLATFLSDRGYLPRGVPLMKRVAALPGQRVCRSGASVIVD
                     GIVIGEALARDRLGRPLPVWQGCRAVAPTELFLMNWQVRDSLDGRYFGPLSAASVIGR
                     ATPLYTDEDGDGRFVWRAPTR"
     gene            complement(13179..13694)
                     /locus_tag="PK264_07860"
     CDS             complement(13179..13694)
                     /locus_tag="PK264_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2840 domain-containing protein"
                     /protein_id="HRD75837.1"
                     /translation="MTRSSTSHAQPVQPSTGPASLTHVELIWREKQIEHWIRFGREVH
                     ERILDRRRRILSFPAGTVFAFVRWASNDYGTVVSRIDIVRAVAACEPYQTLPFVRPGG
                     DILLKLGGWPIVERVLILIDAIEALGIDPADAAPDYWRHVHNRLTAGDTPRAYTREQH
                     RAWLLRRRAEP"
     gene            complement(13691..13933)
                     /locus_tag="PK264_07865"
     CDS             complement(13691..13933)
                     /locus_tag="PK264_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75838.1"
                     /translation="MSKRSFAMRPGDPESWIKAADGRRDSEPNASAYTARLTIDVTPE
                     LRGRIKVAAFQRGQTVADMLRDLLAREFLAADGDAR"
     gene            complement(13930..14568)
                     /gene="parA"
                     /locus_tag="PK264_07870"
     CDS             complement(13930..14568)
                     /gene="parA"
                     /locus_tag="PK264_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HRD75839.1"
                     /translation="MIVALLNQKGGVGKTTLALHLAGEWAGQGKRVILIDADPQGSAL
                     DWSQQRAREGLPRLFSVVGLARDTLHREAPELARDADHVVIDGPPRVAGLMRSALLAA
                     DLVLIPVQPSPFDGWASAEMLALLREARLYRPQLVARFVLNRCGARTIIARETAQTLV
                     DHDPPVLAATIGQRVAFADAAQSGRLVFEATADSPASAEIAALAAEVRRLAP"
     gene            complement(14780..15664)
                     /locus_tag="PK264_07875"
     CDS             complement(14780..15664)
                     /locus_tag="PK264_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HRD75840.1"
                     /translation="MSSRHPGHEREQLDLFRALPGELAPRDAQDLMAYPFFSLAKSKR
                     LAPIDFKAGSIVIRVEAVPEHGMATIWDADVLIWAASQIVEARDIGLRPSRLMATTPY
                     EILTFIGRGVSLRDYDRLKAALDRLQSTTVATSIRQPTERRMHRFSWINEWKERADHR
                     GRPLGLELILPDWFYAAVLDDALVLTIDRAYFELTGGLERWLYRLVRKHGGRQEFGWS
                     FDFAHLHAKSGSLSPLKHFAYDLRDIVRRQPLPGYRLTIEPCVGGPEVLCFAPIDPAS
                     FGTPRRRRRATPRPGDKL"
     gene            complement(15674..15955)
                     /locus_tag="PK264_07880"
     CDS             complement(15674..15955)
                     /locus_tag="PK264_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD75841.1"
                     /translation="MSANPAGLPPRFLRTPEAARFLGLSGRTLEKHRTYGTGPAYRKL
                     GGRVVYSIDDLQAWADRGAVTSTSDPRGTVLPAKRHVSTTPVHAGRHAR"
     gene            complement(16093..16590)
                     /locus_tag="PK264_07885"
     CDS             complement(16093..16590)
                     /locus_tag="PK264_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HRD75842.1"
                     /translation="MIATPGIISAGAPLPALDPSAAHRSTHELHAIQGDGGSAVPFVL
                     IGDVAPDQALAAVIPLDTDAPDRLDAVSRLLGALTTPPPPADLRVTSQRRRRLRHMLR
                     AFDGRTCRATYREIAEVMFGASRVASDAWKTSPLRDATVRLVRDGLAMVNGGYRDLLR
                     RRRRR"
     gene            complement(16538..16861)
                     /locus_tag="PK264_07890"
     CDS             complement(16538..16861)
                     /locus_tag="PK264_07890"
                     /inference="COORDINATES: protein motif:HMM:NF040446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6499 domain-containing protein"
                     /protein_id="HRD75843.1"
                     /translation="MAPDPSQWRSSTSYDYFDDLGVSDLAWECLRRNADYQQDYARAL
                     SKMDDPEQATSAFRLRWGLQFPRQSKPQRDRRADPLDHGGRSRKDPVDRHARHYLGRS
                     TTPRT"
     gene            complement(16982..17245)
                     /locus_tag="PK264_07895"
     CDS             complement(16982..17245)
                     /locus_tag="PK264_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HRD75844.1"
                     /translation="MTAPDFLDEPPRCATATDYDMKHLKLYVRLLDAEADGADWREAV
                     AILFGIDPRDDPERARHVHDSHLLRARWMTEHGYRDLLRKGQH"
     gene            complement(17378..17611)
                     /locus_tag="PK264_07900"
     CDS             complement(17378..17611)
                     /locus_tag="PK264_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD75845.1"
                     /translation="MRLREIVARNLRRLRHAKGLSQEELADRAEINRNYVGMLEREQH
                     AATIDMLEKLAAILEVDPVAFFERPERRGAGKD"
     gene            complement(17791..>17937)
                     /locus_tag="PK264_07905"
     CDS             complement(17791..>17937)
                     /locus_tag="PK264_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
                     /protein_id="HRD75846.1"
                     /translation="RSNEGRDYLGLKLDDPSFTAPIFANLFDDEDGEGYSLIWSRPSR
                     RNGD"
BASE COUNT         3067 a         5571 c         6360 g         2939 t
ORIGIN      
        1 gatcactgtg ccgcccgccg cgagcccggc gccgacggcg gagccggcgc cgagttgcgg
       61 gccgcccgag acgaggccat tggcgatccc gggtccgaag atgccgagtc cgaggagcga
      121 cagcgccgcg aggacgatcg ccatcgcctc gtcgatcgtc ggtgtcgtgc cgccgaagcc
      181 tgcggtgaac tcagagaaga gtgtggagcc gatgccgatg atgacggcga gcaccagcac
      241 cttgatgccc gacgagatga cgttgcccag cacccgctcg gccatgaagg cgctcttgcc
      301 gaagaggccg aaggggatga gcacgaagcc ggcgagcgtc gtcagcttga actcgatcag
      361 cgtgacgaag agttggatgg cgaggatgaa gaaagcgagc agcaccagcg cccaggcgag
      421 gaacatgcag gcgatctgga tgaagttctc gaagaacgac cagtagccca tcaggtcgga
      481 gatcgagtcg agcagcggtc gtccggcatc gaggccggtc tgcgcaacct tgcccggacg
      541 cagcagatcc gccgtcgaga agccggtgcc cgaggctttc aggccgaggc cggcaaagct
      601 ctcgaagacg atgcgggcga gattgttcca gttgccgatg atgtaggcga agacgccgac
      661 gaacagggtc ttcttgatga ggcgcgccat gatgtcgtcg tcggcgcccc aactccagaa
      721 tagcgcggcg agcgtcacgt cgatgacgat gagcgtggtc gcgatgaagg cgacctcgcc
      781 ggaaagcagc ccgaagccgc tgtcgatgta gcgagtgaag acctcgagga aatggtcgat
      841 gacgccggtg ccgcccatgg tgctattgct cttcttccgg cacgggcgcg ctttccttgc
      901 ggactggatc gcctgcgggg tcctcaatgg aggtctcgga ctggggccgg ggcacggcca
      961 ctggatcggg aacacgcccc atcggccgcg cgccgggggc gaggaagcgc tcgcggttct
     1021 cggcccaggc gcgcagacag gccgggtcgc gaggcccggc ctcgccgaga ccctgacagc
     1081 gcatcagctc ggcgcgaagc ggatcggccg gcgctgccgc cataggaccg ctgaccggac
     1141 tcgacggctc ttcctccttc cgggtcatct cgatcgcggt cgcggtaatg gcgagcgcga
     1201 cgaacaccac ggcgccgacg cgggccagca gctttccgtc catgctgccg ccctcagttg
     1261 ccgctgtaga acatcttggc gttgccgggc tgatagcccg agccgggtgt caggaagcgc
     1321 cggcgctgct cgcggccctg ttcggctgcg gccgcccgct cggcctgcga cagcgcttcc
     1381 gcacggccgt tggcggcgac gacagcggtg agatcggcga gctgctgcgc ctggagggcg
     1441 agaagctggt tgccggcctg cgtcgcctgc aaggcgcccg tcgcgccctg gctctggccg
     1501 atgagcgcgg acatttccgc gcggttggtg tcgatgttgc cgacgactgt cgcctgcacg
     1561 cgcatcgcgt cctgcaggcc gccgaccgtg ttctgccagc gcgtgcgtgc gtctgcgatg
     1621 agctgctgat cggacgccga gagcgaggca ttgccgtagg tcgtctggaa ggcgcgatcg
     1681 atctgctgga cgtcgaaggc gatgttctgc gcctgagcga ggagctgctg ggtacgctgc
     1741 accgactgct ggagctgctg aagcgacgag taaggcaggc ttgcaagatt gcgcgcctgg
     1801 ttgatcagca tctgcgcttc gttctgcagc gaggtgatct gattggtgat ctgctcgagc
     1861 gcgcgagcgg cctgcagcac gttctgggca tagttggtgg ggtcgtagac gatgatcgcg
     1921 tgcgccggcg tgacgaacac gggcgagacg gttgcggtga gcgaaaggaa tgcagcggcg
     1981 atggccgcgc gcgagcgacg gtatttcatg gcagggtctc caggttggtg aggtcgggaa
     2041 tgaggtcggc ggcccagtcg acgccgcgat ggcgcagcca ggccccaagg aatccgtcgc
     2101 ggccgtgctc ggctacgatg cggtcgatcg cggcctggtc ggtcttcgac gacgccgcgc
     2161 agagcgcgag cgccacatcc gagaggccga gctcgaagag ccggttgccg cgccgcgact
     2221 ggcaatagta gtcgcgcttg ggcatcgcgc gggcgaggat ttcgatctgg cggtcgttga
     2281 ggccgaagcg gcgatagatc gcggtgatct gcggctcgat ggcgcgttcg ttgggaagca
     2341 atagccgggt ctggcagctc tcgatgatcg ccggtgcgat cgctgagccg tcgatatcag
     2401 agagcgattg cgtggcgaaa acgaccgacg cgttcttctt gcgcagcgtt ttcagccatt
     2461 cgcggagctg gccggcgaat ccctcgtcat caagggccag ccagccttcg tccacgatca
     2521 gcagcgtcgg gctgccgtcg aggcgatcct cgatgcgatg gaagaggtag gcgagcacgg
     2581 ccgctgccgc gcccgtgccg atcagcccct cggtctcgaa cgcctgcact gcggcggacc
     2641 cgagatgttc cgattcagcg tcgagcagcc ggccgtaggc gccaccgacg caatagggcc
     2701 tgagcgcctg cttgagatcg ttggactgaa ggaggacggc aaggccggtt atggtgcgct
     2761 cggggaccgg ggcggtggcg agcgaggtca gcgccgtcca gacatgctcc ttcacctcgg
     2821 gcgtgatggc gacgccctcg cggacaagga tcgcgacgat ccagtcagcc gcccaggcgc
     2881 gttcgggaac gtcgtggata ccgttgagcg gttgcagcga cacgctatgg tccgcgccgt
     2941 ccgtcaaccc gccgccgaga tcgtgccagt cgccgccgca ggcgagcgcc gcggcgcgga
     3001 tcgagccacc gaaatcgaag gcgaagacct gtgacttcgg atagcgccga aactggagcg
     3061 ccatcagcgc cagcagcacg gacttgcctg cgccggtcgg accgacgacg agcgtgtggc
     3121 cgacgtcgcc gacatggagg gaaaaccgga acggggtgct tccctcggtc ttgccgtaga
     3181 gcaagggggg cgacccgaag tgctcatccc gttccggccc cgcccatacc gccgaaagcg
     3241 ggatcatatg ggcgagattg agcgtggaga tcggcggttg ccggacattg gcgtagacat
     3301 ggccggggag cgaacccagc caggcatcga cggcgttgat cgtctccggc atggcggtga
     3361 aatcgcggcc ctgaacgacc ttttcgacaa ggcgaagctt ctcggccgca atgcgtggat
     3421 cggcgtccca gaccgtgatg gtcgcggtga cataggcctg gccggcgtag tcggcgccca
     3481 gttcctgcag ggcaagatcg gcgtcggccg ccttgttcgc cgcgtcggtg tcgaccagca
     3541 cggaggcttc gttggtcatc acctctttga ggatcgcggc gatcgacttg cgcttggcga
     3601 accattggcg cctgatcttc gtcagcagcc tggtcgcgtc ggtcttgtcg agcaggatcg
     3661 cgcgcgtcga ccagcgatag gcgaaggcga gccggttgag ttcatcgagc agacccggcg
     3721 tagtcgcact cggaaagccg acgatggtga ggatgcgcag atgctggtca ccgaggcgcg
     3781 gctcaaggcc gcccgtgagc ggctgatcgg ccagcagcgc gtcgagatac atcggcgtct
     3841 cgggaacgcg cacgcgatga cgcttggccg agaccgtgga atggagatag gtcagcgtct
     3901 cggcatcatc gagccagccg cattccggca tgaagctctc gacgagctgg aggacgcggt
     3961 tggtgcgatc gatgaagccg cgcagcgctt catgcgggtc gacgcgcttg tcggacttgc
     4021 cctcatagag ccagctttct gcgcgcgcag catcttcggc gggcggcaga tacgtgaagg
     4081 tcaggaagta gctcgactcg aagtgcgaag cctcttcctc gaattcagcc ttgcgctcgg
     4141 catcgacgag ggcggacgca gcatcgggaa agatgctcga tggataggcc gcaacgccat
     4201 gccgctgcgc ctcgacgaaa atcgcccagc cggagcccag gcggcggaag gcgttattga
     4261 ggcggccggc gactgcgacc agttcggccg gtacggctga gtcgagatcg ggaccgcgaa
     4321 aacgggcggt gcgctggaag ctcccatcct tgttgaggac gacgccttcg ccgacgagcg
     4381 cagcccaggg caggaagtcc gcgaggcggg tcgaggtccg gcgatattcg gcgaggctca
     4441 tcatggggcg gctcctcaga ccgaaaggtg gccggggatg cgcagatggc ggcgcaccac
     4501 atcgacgaat tgcggatcgc gcctggctgc ccagacggca gcgaggtgtc cgatggccca
     4561 gagaccgagg ccgaccagcc agaggcgcag gccgagcccg agcgcggcgg ccagcgtgcc
     4621 gttgaggatg gcgatcgaac gcggagcgcc gccgagcagg atgtgctcgg tcagcgcccg
     4681 gtggaccggc acggtgaagc ccggaacctc gccggcgctg tcggccacgc ccgtcatcag
     4741 acgagcgctc cgccgccgaa ggagaagaag gacaggaaga agctcgaggc ggcgaaggcg
     4801 atcgacaggc cgaagacgat ctggatcagc cttcggaatc cgcccgaggt atcgccaaag
     4861 gcgagcgtca ggccggtgac gatgatgatg atcaccgcga tgatcttggc cacggggcct
     4921 tcgatcgatt cgaggatcga ctgaaggggc gcttcccatg gcatggacga gccggaggca
     4981 tgggcagcgg gcgcgagcgc caggctgaaa aaggcggcgg ttgccgccgc cgagatgtgg
     5041 cggctgaggc gcgggcaatg acgcatcatg aggggtctcc tgtcaggggg tgtgttgcgg
     5101 gggtgatccg gtaatcgccc tcggccgtga ggccctcgac gcgggcgagc tcggcaaggc
     5161 gacgggttga gccgcggccg ctcagcactg cgacaagatc gatggtctcg gcgatcagcg
     5221 cccgcggaac ggtgacgacg gcttcctgga tgagctgctc gaggcggcgc agcgcgccaa
     5281 gggcggtgcc ggcgtgaatc gtgccgatgc ctccgggatg tcctgtgccc caggccttca
     5341 agaggtcgag cgcctccgcg ccacgtacct cgccaatagg aatccggtcg ggacgtagac
     5401 gaagcgagga tcggacgagt tcggagaggg tggcgacgcc gtctttggtg cgcatcgcca
     5461 ccaggttggg ggcagcgcat tgcagctctc gggtgtcctc gatcaggaca acgcggtctt
     5521 cagtcttggc gacttcggcc agcagcgcat tggtgagtgt cgtcttgccg gttgaagtgc
     5581 cgccggcgac gaggatattg cgccgctcgg ccacggcctg gcgcaggatc gcggcctggt
     5641 cggccgacat gatcgtggtt gcgacatagt cctgcagcgt gaagacggcg acagccggct
     5701 tgcggatcgc gaaggcgggc gccgccacga ccggcggaag gaggccctcg aaccgctcac
     5761 ccgtgccggg cagctcggcc gatacgcgcg gcctaccagc gtggacctcg gcgccgacat
     5821 ggtgcgcaac gagccgcacg atgcgttcgc catcggccgg cgacagccgc tcccccgtat
     5881 cggcgaggcc ctcggacagc cgatcgatcc agagtcgccc gtccgggttc agcatcacct
     5941 cgacgatcgc ggggtcttcc aggaatcggg cgatcgacgg cccaagggcc gtgcgcagca
     6001 tgcgtgcgcc gcgcaggata gcttccgatt gctggtgagt ggccgccacg tcgtccccgt
     6061 tctctggcgg gccttcgcaa cgcgcggccc tggatcgggg atgagtagaa gaggcagaaa
     6121 tttggccacc gcaacaaggc cgtgggcgta gtagcgatgt agcgtagaaa gacagggaaa
     6181 cggcgagagc gcttgccgca acaggtgagc acgccggctc tgttcacgat cgaaagtgaa
     6241 ggcccgcggg tcgccgattc cagcgacatc agttccgaaa gcctatagcg gtctattcag
     6301 ttgctgtccg ggaggaagcc caaggtgcgt gcttgggcga gtgcctgttt gttgctcgcc
     6361 gggatccacc agagcctctc cgccggcgac cagcgagcgc cgagtttctt ggcctcctcc
     6421 ttgcccgaga aggggaccga aagaaggacg cgttcttcca ccttctgctc ttcgcgaggt
     6481 gccttgtggc gttttcggtc aagggattga agagcggcac tgatggcggg atcgtcgagc
     6541 gatcgcaagt tggcatgctc tgcccatgaa ggcgcaggaa ttctccgcca ggtcttgtaa
     6601 gtgccgggga ccgatacttg cgccaggttc tccctcaact ctccggccaa gctatagacg
     6661 tccatcaaaa cggaagggtt gccgaaatat ggaccaggct tcgtcatcgc gccgacaccg
     6721 acaacgcgcg cgcaggtcgc tccccacgtc gttcgacgga tccagaagcc gggacctcca
     6781 ttcgtgacac acatctcaaa gatgttttga tcgctgctat ctcgccagtc gtcgctgccc
     6841 atgtcctcaa gtcttcgcca tgccttcgat gtagagttgt ctccattcta taagaaaatc
     6901 gaagcgcagg ctctattgct cacacgcagt ccgtcggcga agagggtgcg ccgccgaacc
     6961 acttgccggc ggctgaaacg gcggctggtg tgtcagattc agttcactaa cggtcgtctt
     7021 cgtgcgcctc ggttggaggc gccgacgtgc cgccagcaac atcgtccggt atctccttga
     7081 ggaagctctg acctttctgg agtcggcggc cgagcgtttg gatgaagcct tcatagcgct
     7141 cgcgcccctt gatctgcgca gcgccctgtc cgtcgttcgg caacggcggc gtgacggtga
     7201 gccagaagcg gacgaacagc gcgagcgttt ccgccgtgac gccgaggtcg cgttcgagcc
     7261 gctggacctg gcgcgaaagg cggtcgaggc ggcgcgtgaa cgccgcctcg cgcttgtccg
     7321 cgccatcggg tgacaggaag gaggcgaccg cagcctcgac gatcgccgag cgcgagagct
     7381 tcttgcggtc ggcgaggtcg gcgatctggc gcagcatctc cggcgggaaa tagacattca
     7441 tccggtcgcg catggccgcc tcacatatcg atgccgtcat tgggatcgag cgcgacctgc
     7501 cgggcgacgc cgcgcatctg ctggcgcagg atctggcgct ggcgcgccgc gtcttccggc
     7561 gcgtcgtcga gaacgatggc gaactcctcg gccggcgcct gctcggtggt ctccttcacg
     7621 atggcgacgt gatcgggaag ctcgggctca cgccgcaagc cgctattggc ggcgtcctcg
     7681 gccttctcga tctcggcgag cagcacggtg tcgggccgct gcggcttcag gtcggaccag
     7741 ccatccttgc gcgtcgaccg gccgcatttt gccggatcgg gcgatgtgag gacacgctcg
     7801 gtgaaacggc gatcctcgta gtagcgcgcc ttctttgccc ggatcggatg cacgccggcg
     7861 gccatgacga tctcatcggt cggtggaagc tgcatcactt cgccaggggt gagcaggggc
     7921 cgggctgttt ccgagcgcga caccatcaga tggccgagcc agggactgag ccggtggccg
     7981 gcatagttgc gcatcgcctt catctcggtg gcggtgccga gcgcatcgct cactcgtttc
     8041 gcggtcctct catcgttggt cgcgaagctc acgcggacgt ggcaattgtc gaggatggag
     8101 ttgttcggac cgtaggcctt ctcgatctgg ttcaacgact gcgcgatcag gaacgatttc
     8161 aggccatagc ccgccatgaa ggcgagcgcg ctctcgaaga agtcgagcct tccgagcgca
     8221 gggaattcat cgagcatcat cagcacgcga tggcgccggt ccttcgcgtg caggtcctcg
     8281 gtcaggcgcc ggccgatctg gttgagcacg aggcggatca acggtttcgt ccgcgagatg
     8341 tcggacggcg gcaccacgag gtagagcgtc gccggtttag gatccgcgat caggtcggca
     8401 atccgccagt cgcagcggcg cgttacctcg gcgacgacgg gatcgcggta caagcccagg
     8461 aacgacatgg cggtggacag aacgccggag cgctcattgt cgctcttgtt gagcagctcg
     8521 cgggccgtcg aagcgacgac gggatgcggg ccagccttgc cgaggtgcgg cgtcgtcatc
     8581 atcgccttca gcgtagactc gatcggtcgg cgcgggtcgg acaggaaggc ggcgacgccg
     8641 gcgagggtct tgtcttcccc cgcatagagg acatgcagga tcgcgcccac gaggagcgaa
     8701 tggctggtct tctcccagtg attgcgcttg tcgagcgagc cttcagggtc gacgagcacg
     8761 tccgcgacgt tctgcacatc gcgcacctcc cactcgccgc gtcgaacctc gaggagcgga
     8821 ttgtaggcgg ccgacttggg attggtcgga tcgaacagca gcactcgacc gtgcttcgcg
     8881 cgaaaaccgg cggtgagttg ccagttctcc cccttgatgt cgtggacgat ggcggagccc
     8941 ggccaggtca ggagcgaggg cacgacgagg ccgacgcctt tgcccgatct ggtcggcgcg
     9001 aagcagagga tgtgctcggg tccgtcatgg cgaagatagg catcgccgag cttgccgagg
     9061 acgacgccat ccggtccgag caggcccgcc gccttcacct cccgcaactc cgcccagcgg
     9121 gcggagccgt aggtctcgac gtttttcgcc tcgcgggcgc gccacaccga catgagaatc
     9181 gcgacggcga tggagatgaa gccgcccgag gccgcgatat aggcgccctc cacgaagatc
     9241 ggcggcgcat aggcgtcgta gaagtaccac caccagaaga aggccggcgg ataatagatt
     9301 ggccagcccg ccagctcgaa ccagggttgt ccgagcccag gctggaagcc gagccgccag
     9361 gccgtccact gcgtcgccgc ccaggtcgtg aacagcacga tcaggaagac ggtggtgatc
     9421 tggccccaga ggattttcgt cgcagacatg gccggctttc ctttcttgct tgtaggttag
     9481 aggccgagcc cccgtttgcg gccgagactc cattcaatac cgccgccgtc cttcgcgacg
     9541 ccggcgatat gctggccgag cttgcgctcg atctcgcgag accagggcac gagctggaag
     9601 ccgagcccgt tgtcgatcat cgcgaagcgg ccggaagaga gcgtcagccg ctggcgatag
     9661 actcccgcaa catgctcgcc ggctgcagct ttcatatgcg cgaggccggt ttcggccgag
     9721 agcttcgcgc ctacggcgtc gagctcgcgc cggcgcaggg tattcaggag atcgcgccgc
     9781 aggatgatgc gctccccttg tcggcgggcg agaccttcgc ctgccagatg ctcggctcgc
     9841 gcctgcatgg cgtcgcgcac ctcgcgcccg aagccgccca tggagagcgg catgggctcg
     9901 cgctcgacga gacgatgatc gagccaggtg gcgcccggcg ccgtgacctg ccggttgaga
     9961 tcgagatcgg atcggttggc gagcaccagc gtcggccgcc gctcgtccgc cccgccgaag
    10021 cacctcacct cgacgatgcc gccgatcggc ggcgcatgct cgaatgcctc gattccccgg
    10081 aagcggacat gatgggcgcg gccgtcggtg ccgtcgatca ccgcataggc ttcgccggtc
    10141 agctcgtcgt gcaagccgcg gtcgaccagc cggccaatga tcggcgcctg tgccgcgcct
    10201 ccctcaatca catagtcggg gacgccgcgc tcctggccgc gttcagcgaa ggcgcgatgc
    10261 atggtcttga tgatgtcgcc ccgcatgccg aggtcgcgca gatgccgttc ggcgtcgagc
    10321 ccaaccatcc actcgcccgg gctggcggct gtcgcgaggc ccattttctc cagatgctgg
    10381 agccggccga tcatcaggcg gcgtgtctga ggatcggagg cgccgcgttg ctcgggccgc
    10441 aggtcgatgt agcctgtctc gtcggcggcg atgcggatct cgacgtcgag gcgcgtccag
    10501 cgctccgccg tgatctcccg ttccagcgca tggcggatct cgtgctcggg cttcgggccg
    10561 agctccatgg agacgagatc ctcggcgcgc gagcgcaggc cgcggctgat atagtcgcgc
    10621 gatatgacga ggtcggcccc tttgtcgtcc acaccgcgca caagcagatg gacgtgcgga
    10681 ttgtcggtgt tccagtgatc gacggcgacc caatcgagcc tggtgccgag atcggcttcc
    10741 atctggctcg cgaggtcgcg ggtaaaggcg cgaaggtcgg tcatgtcgcc ggcgtcctcg
    10801 ggcgagatga tgaagcgaaa gtgatgccgg tcatctttgg ctcgcgccgc gaagcccgcg
    10861 tcgtcggcgc tgtcgctgcc ggcgtcgaac atcaccgcct tctcgccatc gcgtgtaacg
    10921 ccgtcgcgct tgagatagga gagatgggcg gcaagcggcg ctgagcggaa cgctcggccc
    10981 tgatggcggg ctatccgggc tttcaccacg acgcggcgcg aagggctgaa caggcgcgcg
    11041 cggctgaagc tgacgcgtcc gcgtccgaag gtcgagcgcc catagctgga cgccctgcgg
    11101 cccggtgcgc catgagcgga gacatggccg gcgcgtttgg cggcgcgcag gacctgattg
    11161 atgaagcgct ttggcttcgg cgcacgcgtg gagcggatgc ggccgggccg gatgcggaag
    11221 tcgctgtcgc cctcgctcat ggccgggctc cagcgggcgt tcgggcgcac agtgccgaaa
    11281 acatccctga aattgttggc gaaagtccaa ggcgcggcac cgcacccgac cgaccggcac
    11341 gcgaaaagcc atgcgaaagc aatggcttgc gccagcgtag cgagccgctt ttatctcgcc
    11401 gtcggttggt cgtgttgacc tctctccggc cgcttcccgc ttctccttcc cgggagcccg
    11461 ccagagcgcg ccagaatgcg aacgctgtca tcgcggacct ccgccgttcg atcgcgcgac
    11521 gaagagaccg ccggtcgggt gctcggcagc atcgtcagag tcctctgggg ttgcagcgcg
    11581 atcgctactg gattgacgct cgttcagcgc cggatcggca gcgccgccgc tgtaggagtg
    11641 cgcaacgaac agcggtgcgc gcgtccattc gagcggattg gcagcggcta cgacgacatg
    11701 ggcaagcggt tcgctgccga ccagcggcgc gagggcggcg acataggcga ccgtttcggc
    11761 gggcagtgga cggccggacg cgacataatc gtcgtagcgg ccgggtcccg cattgtaggc
    11821 cgcgaggaag ccaggcgcgc cgtaacggtc gtgcatctcg cgcagatagg ccgcacccgc
    11881 caggatgttg tcgcgtgggt cgtaggggtc gacgccaagg ccgtagcgcg cacgcaattc
    11941 ggcccagctg tcgggcatga tctgcatcag gcccatcgcg cctttgggag agaccgcgcg
    12001 aacgcggccg cggctttcga cacgcatgac ggcccagatc cagcgctcgg ggatgtcgaa
    12061 gcgccgcgcc gcctcggcga cgaacccggc atagggtgag acatcgcgtg gagcctcggg
    12121 cggtcccgcc tgggcgggcg gaaccaatgg cgtcgctcca gcgcagatga ggccggaaag
    12181 gagaagaagg gtcagatgtc gggcggggag tgaacgcccg gcggcgaact ggcggcgcgg
    12241 cgcggacatc ggtcagtccc gctcgccacg cttgggcggg cggttccaat gcagtcccca
    12301 ggcggacttg tcgtcggccg attggaagag gttggcgcgg accggctgtc cgaaggtcgg
    12361 atcgtcgatc tgcagcgaga catagtcgcc ggccttctcg ccagtgcgct tccagcccgc
    12421 accgatctcc ggcccgtcgg cgtcgctgcc gagatgaatg cggtagtcgg gcgcgttctc
    12481 ggcgtcggaa tgctccgccg gcacgagcac caactcggca tcgagaccaa gcgtgcgaac
    12541 acgtccggca tagccgttcc tggtgcgcga gaactgaccg atcaaagcca tgatatcctc
    12601 cttgggcttg cggtggggtt ggtcatgggc gcaccgtcac cgcgtcggcg cgcgccagac
    12661 gaagcggcca tcgccgtcct cgtcggtgta gagaggggtg gcccggccga tcacggaggc
    12721 cgccgaaagc ggcccgaaat agcggccgtc gaggctgtcg cggacctgcc agttcatgag
    12781 gaacagctcg gttggcgcga cggcgcggca gccctgccag accggcaggg gtcggcccag
    12841 gcgatcgcgt gcgagcgcct cgccgataac gatcccatcg acgatgacgc tcgcgccgga
    12901 tcggcagacg cgctgtccgg gaagcgcggc gacacgcttc atcagcggca cgccgcgcgg
    12961 cagatagccg cgatccgaca ggaaggtcgc aaggggttcg ggcgcatcga ccgcgacgag
    13021 gtccctgacc tcgagcggtg tcgaagcgcc gatggtgtag agcccgacgg gcgcgctggc
    13081 cgaggcgttc cagacgagtc ggggcgcgac gtgaaccagc gacagcgcgc cgatcagcag
    13141 cacggcgaca gatgtcgtca cgacatagcc gaagcgcgtc acggctcggc cctccgccgc
    13201 aggagccagg cgcggtgctg ttcgcgggtg taggcccgcg gcgtatcgcc agcggtcaag
    13261 cggttatgga catgacgcca gtagtcgggc gccgcgtcgg cgggatcgat gccaagcgct
    13321 tcgatggcgt cgatcaggat cagcacccgt tcgacgatcg gccagccgcc cagcttcagc
    13381 aggatatcgc cgcccggacg cacgaagggc agcgtctgat agggctcgca cgcggccacc
    13441 gcgcgcacga tgtcgatgcg cgagacaaca gtgccatagt cgtttgaagc ccagcggacg
    13501 aaggcgaaga ccgtgccggc cggaaaggag aggatgcggc gccggcggtc gagaatccgc
    13561 tcatgaactt cgcggccgaa gcgaatccaa tgctcgatct gtttctcgcg ccagatcagc
    13621 tcgacatgcg tgaggctcgc cggtcctgtc gatggttgaa cgggctgcgc gtgggatgtt
    13681 gacgatcggg tcatcgcgcg tctccgtcag cggcgagaaa ctcgcgcgcg agaaggtcgc
    13741 gcagcatgtc ggcgacggtc tgcccgcgct ggaacgcggc gaccttgatc cggccgcgaa
    13801 gttcgggcgt gacgtcgatc gtcaggcggg cggtgtaggc ggaagcattg ggctcgctgt
    13861 cgcgtcgccc atcggccgcc ttgatccagc tctctggatc gccagggcgc atagcgaagc
    13921 tgcgcttgct catggcgcga gcctccgcac ctcggccgcg agcgcggcga tttcagccga
    13981 ggccgggctg tcggcagttg cctcgaagac gagccggccg gactgggcgg cgtctgcaaa
    14041 ggcgacacgc tggccgatcg tggcggcgag cacgggcgga tcgtgatcga cgagcgtctg
    14101 ggccgtttcg cgtgcgatga tggtccgcgc gccgcagcgg ttcagaacga accgcgcgac
    14161 gagctgcgga cgataaaggc gcgcttcccg aagcagcgcc agcatctcgg ccgaagccca
    14221 tccgtcgaac ggcgacggtt gaacggggat gagcacgagg tcggcggcga gcagtgccga
    14281 acgcatgagg ccggcgacgc gcggaggtcc gtcgatgacg acatgatcgg catcacgggc
    14341 gagctccggc gcttcgcgat gcagcgtgtc gcgtgccagg ccgacgacac tgaagaggcg
    14401 cggcaggccc tcgcgggcgc gctgctgcga ccaatcgagg gccgagcctt gcgggtccgc
    14461 gtcgatgagg atgacgcgct ttccctggcc ggcccattcg ccggcgagat gcagcgcgag
    14521 cgtcgtcttg ccgacgccgc ccttctggtt gagcagcgcg acgatcatgg cgcgctcctc
    14581 ggcgctcgcg gacagttatc cgcaggcgtg caaaagcgcg tgtccgttag aaagattccg
    14641 aaggaatctg ggttagagaa gttacgggac ctgcaaactg ctgattcagc ttggcgattc
    14701 gacgattccg gtccccgata gcacgagagt ccggtccccg atggcacgat agtgccggtc
    14761 cctgatagca cgaggcgatt cacagcttat ccccagggcg tggcgttgcg cggcgacgtc
    14821 ggcgcggtgt tccgaaggac gcgggatcga tcggcgcgaa gcagaggact tccgggccgc
    14881 ccacgcatgg ctcgatggtc aggcgatagc cggggagcgg ttggcggcgg acgatgtcgc
    14941 gcaagtcgta ggcgaaatgc ttgagcggtg agaggctgcc ggacttggcg tggagatgcg
    15001 cgaagtcgaa gctccagcca aactcctgcc gcccgccatg cttgcgcacc agacgataga
    15061 gccagcgctc gagcccgccg gtgagctcga agtaggcccg gtcgatggtg agcacgagcg
    15121 cgtcgtcgag aacggcggcg tagaaccagt cgggcaggat cagttcgagg ccgagcggcc
    15181 ggccgcggtg atcggcgcgc tccttccact cattgatcca ggagaagcgg tgcatccgcc
    15241 gctcggtcgg ctggcggatg gaggttgcca ccgtggtcga ctgaagccga tccagcgcgg
    15301 ccttgagtcg gtcgtagtcg cgcagactga cgccgcggcc gatgaaggtg aggatttcgt
    15361 agggggtcgt cgccatcagg cgcgatggac gcaggccgat gtcgcgcgcc tcgacgatct
    15421 gggaggccgc ccagatcagc acgtccgcat cccagatggt cgccattcca tgctcgggca
    15481 ccgcctcgac acggatgacg atcgagcccg ccttgaaatc gatcggggcg aggcgcttcg
    15541 acttggcgag cgagaagaac ggataggcca tgaggtcctg cgcatctcgc ggcgcgagct
    15601 cgccgggcag cgctcggaag aggtcgagtt gctcgcgttc atggccagga tggcgggatg
    15661 acatgccggc gcctcagcgg gcgtggcggc cggcgtggac cggcgttgtc gatacatggc
    15721 gcttggcggg taggacggtg ccgcgcgggt ccgacgtgga ggtgacggcg ccccgatctg
    15781 cccaggcctg caggtcgtcg atggagtaga cgacacggcc gccgagcttg cgatacgcag
    15841 ggccagttcc gtaggtccgg tgcttttcga gggtgcggcc cgaaagaccg aggaatcggg
    15901 cagcttcggg cgtgcgaaga aagcgcggcg gcaagccggc gggattggcg gacatgagcg
    15961 gacctccgtg gcgttcgttg aggccgccgg cgaacggcgg cgaacgaagg ccacggtggc
    16021 gaagagctgc cggctggggg gatggtgaag atagggggtg ctatttcgca cccccctgaa
    16081 accggcgctc cgtcagcgcc gtcggcgccg acgcagcagg tcgcgatagc cgccattcac
    16141 catggcgagg ccgtcgcgca cgagccggac ggtggcatcg cgtagtggcg atgtcttcca
    16201 cgcgtcggac gcgacgcggg aggcgccgaa cattacctcg gcgatctccc gataggttgc
    16261 acggcaggtc cggccatcga atgcccgcaa catgtgacgg aggcgccggc ggcgctgcga
    16321 ggttacacgc aggtcggcgg gcggaggcgg cgtcgtcagc gcgcccagga gacgcgagac
    16381 ggcatcgagc cggtcgggcg catcggtgtc gagcgggatg actgcggcaa gcgcctgatc
    16441 gggagcgacg tcaccgatca ggacgaaagg gacggcgctg ccaccgtcgc cctggattgc
    16501 gtggagttcg tgcgtgctgc ggtgtgccgc gctcggatca agtgcgggga gtggtgctcc
    16561 ggccgagata atgccgggcg tggcgatcaa caggatcttt ccgggatctg cctccgtggt
    16621 ccaatggatc ggctcgtcta tcgcgctgcg gcttggattg gcggggaaat tgcaaccccc
    16681 agcgcaggcg gaacgcactc gtagcctgct cggggtcatc cattttgctc agcgctcttg
    16741 cgtagtcttg ctgataatca gcgttgcggc gcaggcactc ccacgcgaga tcggaaacac
    16801 cgagatcgtc gaaataatcg taactggtcg aggatcgcca ctgcgacggg tccggcgcca
    16861 tcggttacct cctggctcac atggttcgtg tatccgggcc ggctacgatc cccggttgag
    16921 ctggatcagg gaagtcctcg gatgagcgtc acgtgatcgc tcgaatgcat cgcattcgag
    16981 ctcaatgctg gccctttcgg agcaggtcgc gatagccatg ttccgtcatc catcgcgcgc
    17041 gcaggagatg actgtcgtgg acgtgccggg cacgctcggg atcatcgcgg gggtcgatac
    17101 cgaacaaaat ggcgacggct tcccgccagt ccgcaccatc ggcctcggcg tcgagaaggc
    17161 ggacgtaaag cttcaggtgt ttcatgtcat aatcggtggc cgtcgcgcac cggggcggtt
    17221 catcaagaaa atccggcgcg gtcatgatgg gtcccccgac acatcaacgg cgacttccat
    17281 tcgatctgaa ctcaatttta accaagtttt gaatgggcgt aagctgcacg gcgctgcatc
    17341 gcgtgatgca gcagagacat cgccgcgcgc tgcgcaatca atctttgccg gcgccccgtc
    17401 gctcgggccg ttcaaaaaat gcgacgggat cgacctcgag aatggctgcg agcttttcca
    17461 gcatgtcgat cgttgcggca tgctgttcgc gctcgagcat gccgacataa ttgcggttga
    17521 tctcggcacg atcagcaagc tcctcctgcg acaagccttt ggcgtgtctc agccgacgca
    17581 aattcctggc gacgatctcc cgcaatctca tgcggtgagg tcgccgttcc ccctaatata
    17641 ctgccaccta gtaaactagt gattcgtgat gccggaaagg cgcaaagtca cttttctggg
    17701 atgatcggga gcattcggcg cgctctcgga cggaattccc acagcccgat ggccccgccc
    17761 ggaccgtccg ggcggggcat cggtggcggc tcagtcgcca ttgcggcggc tggggcggga
    17821 ccagatcagg ctgtagccct cgccgtcctc gtcgtcgaag aggttggcga agatcggggc
    17881 ggtgaagctc ggatcgtcga gcttgaggcc cagatagtcg cggccctcgt tggagcg
//
LOCUS       DAORNH010000056        17811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2408_length_17811_cov_2.732485, whole genome shotgun sequence.
ACCESSION   DAORNH010000056 DAORNH010000000
VERSION     DAORNH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17811
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_17811_cov_2.732485"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PK264_07910"
     CDS             complement(<1..509)
                     /locus_tag="PK264_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HRD75847.1"
                     /translation="MSRIRPASYSPLQKSLHWLIALLVVGMIGVGLYMVRRGVATKFD
                     AVTAQLYTYHKTIGFAVLWLVVLRAIVRLTRGVPAPEPSLNLVQRLAAEATHLAIYGL
                     LIAVPVLGWIGVSAYGAPQIVGGYSLPALVAQDERMAKTVLWLHGWAAIGLGLLVLAH
                     VGAALMHKVV"
     gene            complement(506..1219)
                     /locus_tag="PK264_07915"
     CDS             complement(506..1219)
                     /locus_tag="PK264_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HRD75848.1"
                     /translation="MDYEHCFSPLREAGAGRSFVVAQLGQSLDGRIATPTGESRWING
                     HAALVHVHRLRSEVDGVLVGVGTAIADNPQLNVRHVTGRNPTRVILDPSGRMPSDLKC
                     LAADGARRIVVRACGSRDGGSPYPNGVETIELPAAAGVISPLEVVAALARTGLHRILI
                     EGGAHTVSAFVNAGAVDRLHILMAPVILGSGKSGLALQPIERLSEALRFRADVHVLDG
                     GDVLFDCDLRSRAVEGLTP"
     gene            complement(1381..1941)
                     /locus_tag="PK264_07920"
     CDS             complement(1381..1941)
                     /locus_tag="PK264_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HRD75849.1"
                     /translation="MSWSDERVELLKKLWAEGLSASQIATRLGSVTRNAVIGKVHRLN
                     LAGRATKSRVKAIRPRVKTAAPGGSQIAKRPQARAKFAQLGNPLIRQLYEADAEPLPS
                     HEELDIPLAERKTIQTLVECSCRWPIGDPQAADFHFCGKNKMPSLPYCEFHARRAFQP
                     PQVRRRDRPTVEPVPTVPGIKQIVRA"
     gene            2610..3482
                     /locus_tag="PK264_07925"
     CDS             2610..3482
                     /locus_tag="PK264_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD75850.1"
                     /translation="MSVFRSFLFAPGNHPRRVEKALGLSADAVILDLEDACPIAEKVA
                     TRAVVVTACQRARSGLGYIRVNAMSTEFGYGDMVAVVQSGVDGIILPKLETADEIRAA
                     DWVIANLERERGLKSGAFDIIPIIETARGMANLRAIAAAGTRVRRFAFGAGDFTLDLS
                     MEWTRAETELLPYRSECVLAARAGNLEAPIDTVWVDLKDPEGFRASARLVKGLGFQGK
                     MCIYPDQVPVVNEVFTPTAADVEWSRRVVAAFKEAEKAGSASIQLDGKFIDYPIVYRA
                     ERVIQMMDRIAARR"
     gene            complement(3488..4492)
                     /locus_tag="PK264_07930"
     CDS             complement(3488..4492)
                     /locus_tag="PK264_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRD75851.1"
                     /translation="MPGTITVREIFSIAERALSKAGARPEVARSMAKAIAAAERDGIA
                     SHGLAYLATYCEHLQCGKVLASAVPAVTTPAPAVVAVDAASGFAHPAIDAGFARLIPL
                     AKAQGIATLVIRNSYNCGVLGHHTERLAEAGFVALGFTNAPASIAPSGGRKAVVGTNP
                     WSLAVPDGKGGVAIAIDQSASVVAKSEVMKKARSGQPIPQGWALGPDGQPTTDPNVGL
                     KGTMMPAGGYKGVSSAILVEVMAACLSGATLGIAASPFSGTAGGPPKTGQCFMAITPD
                     APSGGAFQARIADLVTAIAAEPGTHLPGAGRKAARARADVNGVAVDAALIEKARALGV
                     "
     gene            4646..4855
                     /locus_tag="PK264_07935"
     CDS             4646..4855
                     /locus_tag="PK264_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HRD75852.1"
                     /translation="MPALHRDYNLADGDAIYAELVAAHRGLDERQSAALNARLVLILA
                     NHIGDTAVLREAIAAALASASSSPG"
     gene            complement(4856..>5552)
                     /locus_tag="PK264_07940"
     CDS             complement(4856..>5552)
                     /locus_tag="PK264_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75853.1"
                     /translation="NDPAITAHYRVLVPDMPWHGKSLPPVGHEREEYRLTTAAYTEWI
                     LSLCSALELARPVIMGCSIGGRIVLNLAAEHPERFRALIGLESAAHQQPWYDTDWLHR
                     PDVHGGEVCAGLVSGMMAPQSPAESRHETLWMYKSSGPGVFKGDLYFYRVDGDLRAKI
                     AKIDTRRCPLYLLTGEYDFSCTPDDTRRTAEAIAGVEVTIMERLGHFPMSENPAHFRS
                     YILPVLEKIRNRP"
     assembly_gap    5553..5562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5563..5757)
                     /locus_tag="PK264_07945"
     CDS             complement(<5563..5757)
                     /locus_tag="PK264_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75854.1"
                     /translation="MAPRFEPITGRYFSLELGARAHRVYVEEAGQGIPLLCLHTAGAD
                     GRQFRHILNDPRWDGIAMLLE"
     gene            complement(5761..6156)
                     /locus_tag="PK264_07950"
     CDS             complement(5761..6156)
                     /locus_tag="PK264_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75855.1"
                     /translation="MTSAVVAAFSRLPELAAADADIARRGRHATLDVAIGVGSETLIA
                     RIVGGDVKDVLRGPFLLRPWTIAIRAEPEVWQAFLSDPPPPGSHDILALSKRGALRIE
                     GNLQPFMASLQVVKDILALPRGMVGTHKS"
     gene            complement(6156..6752)
                     /locus_tag="PK264_07955"
     CDS             complement(6156..6752)
                     /locus_tag="PK264_07955"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRD75856.1"
                     /translation="MAALLLPVPALAHHPMGGKRPETILQGLLSGIGHPVIGPDHLAF
                     IVAIGFASAFVRGGLVVPMGFVISSALGVLAHAKGMPLPMVELLVALSVLAVGMLLFT
                     GLQLAGSFWSFLAFNGGLVHGYALAESVLGSEQGVITAYVAGIAVVSFAIAAAAQAFA
                     KVMTGTLGQHHPRVRAAGAVLGCLGAALVVFSLAPVAP"
     gene            complement(6878..7855)
                     /locus_tag="PK264_07960"
     CDS             complement(6878..7855)
                     /locus_tag="PK264_07960"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRD75857.1"
                     /translation="MSAPLLKVNELVKHFPIRGGLLQREVDRVRAVDGVSFEVAKGET
                     LGLVGESGCGKSTTGRCILRLIEPTAGEVWFEGKSVSEMGREQLRALCRDMQIIFQDP
                     FASLNPRMTVGAIIGEALVIHGLAKSKKEHEERVAELLRTVGLQPDHMRRYPHEFSGG
                     QRQRIGIARALAVEPKLIVCDEPVSALDVSIQAQVINLLEDLQEKFNLTYIFIAHDLS
                     VVEHISQRVAVMYLGRIVEIASAHDLYAAPKHPYTEALLSAVPIPDPQVKRRRIMLEG
                     DVPNPIRPPSGCHFHTRCPIRQLPLCSTEAPKLEQGANGHWVACHLRKG"
     gene            complement(7880..8791)
                     /locus_tag="PK264_07965"
     CDS             complement(7880..8791)
                     /locus_tag="PK264_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75858.1"
                     /translation="MVRAVDGVDITIHSGETVSVVGEAGCGKTVTAMSVLKLIAMPPG
                     QIVAGQILWQGRDLVPLDKAEMNEVRAREIAIVFQEPMTSLNPVYSVGDQIAEVIRQH
                     EKVSKREALARTIEMLRLVQIPNPERRVTDYPHQFSGGMRQRVMIAMALSCKPQLLIA
                     DEPTTALDVTIQAQILELLQDMKSRFGMAIMLITHAMGVVAETAQRVVVMYAGKVIEE
                     APVEKLFGNPRHPYTQGLIRSIPRIDRAATHKARLEAIKGVVPSLLRPPPGCRFAPRC
                     NFATDACRAAEPQLREVEPGHKVACIL"
     gene            complement(8893..9264)
                     /locus_tag="PK264_07970"
     CDS             complement(8893..9264)
                     /locus_tag="PK264_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020051647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75859.1"
                     /translation="MLGHISFGVEDLARSIRFYDAALAPLGVTRVWTTDTAAGFGALG
                     GPDRLALFHRPGAARAPGPGFHLALVAPSRDAVDAFSAAALAHGGRDDGPPGPRPRYS
                     PTYYAAFVIDPDGHKLEAVHQ"
     gene            complement(9281..10243)
                     /locus_tag="PK264_07975"
     CDS             complement(9281..10243)
                     /locus_tag="PK264_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD75860.1"
                     /translation="MSVVDQPPPTYSPRNAPEPATAAVAQAAPIYSPAREAWRKFRRH
                     RLAVVGTIVLTLLILAVLIGPYVWKTPINEIDFKAKLQGPSWAHPFGTDDLGQDILAR
                     MLYGGRISLAVGLAAMIVAVVVGTIVGAIAGMSRGWVGTALMWLTDLFLSLPQLPLLL
                     LVIYLFRDALKDVFGPEGGIFILIVIVIGGLRWMPVARLVRAQFLSLREKEFVEAARA
                     LGASKTRQVVRHILPNALGPVIVAGTIDVALAIIIESTLSFLGLGFPPDIPTWGRILF
                     DARDNLDFAPHWALFPGTAIFLTVLSINFIGDGLRDALDPRKVM"
     gene            complement(10325..>10923)
                     /locus_tag="PK264_07980"
     CDS             complement(10325..>10923)
                     /locus_tag="PK264_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD75861.1"
                     /translation="ATRPYSLFDQLANTFAFIGFSLPTFFTGILFILLFSITLGWLPF
                     VYRADIAATGWQWFWEHFKQSIMPIAVLGLFQAASLTRYVRSSVLDVIRLDYVTTARS
                     KGLKERTVILKHVVRNALIPVVTLLALQAPAIFGGAIVTEQIFRIPGIGSLLISSILA
                     NDTPVVMAVTFIFACLVVLFNLIADILYGCLDPRISYS"
     assembly_gap    10924..10958
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10959..11286)
                     /locus_tag="PK264_07985"
     CDS             complement(<10959..11286)
                     /locus_tag="PK264_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD75862.1"
                     /translation="MGMYILRRLLIAVPSLLGISVVLFTVLALAPGDPFGELATNPAV
                     PPEVRAALRAKFGLDDPIHLRYFSWITAMFQGNWGFSFVSRMDVDKLILQALPTTLFV
                     IGSAQVL"
     gene            complement(11349..13139)
                     /locus_tag="PK264_07990"
     CDS             complement(11349..13139)
                     /locus_tag="PK264_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD75863.1"
                     /translation="MEPKSLHRLIDRVKEGSLSRRSFLTRMAAAGLAAPLANQLLALG
                     GVAMAQSKFAYKPTKRGGGGTLKLLWWQGPTLLNPHFAVGTKDQDGSRLFYEPLAAWD
                     ADGNLVPILAAEIPSTANGGLSADGKTVIWKLKKGVTWHDGKPLTADDLVFNWEYATD
                     PGTAAVTIGSYKDVKVEKIDDTSVRVVFAKPTPFWADAFVGTRGMIIPKHLFADYKGA
                     KSREAPANLKPVGTGPYTFVDFKPGDMVKGAAYANYHVANQPHFDAVEMKGGGDAVSA
                     ARAVLQTGEYDYAWNLQVEDEILLRLEKGGKGAVSIVPTGNIEHIQVNFTDPWNEVEG
                     ERASMKSKHPTLADPAVREALNLLVDRKSVQDHIYGRTGIATANFVNNPDKFTSKNTK
                     FEFNIEKAIKVLDDAGWKPGPDGIRAKDGKKLKFVYQTSINAPRQKNQQIVKQAAQKA
                     GIDIELKSVTASVYFSSDVANPDTYTKFFCDLQMYTTTMTQPDPELFMNQFCSWEVAT
                     KANKWQGRNITRWTSKEYDDTYKAGVAELDPVKRAALFIKCNDLVIAARAVIPVVARP
                     QAGGASLKLRPHLSGWDNTTWNIAAWYREA"
     gene            13509..13844
                     /locus_tag="PK264_07995"
     CDS             13509..13844
                     /locus_tag="PK264_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75864.1"
                     /translation="MKIRHAIGIALLSVPFLAASASAQGMSDLHTQVRIGNRICMADH
                     FHTGSGNGASKDAAMRAAISSWSDFTAWEYGNNWGSWRLAETKSSTCEPGFGGGFSCT
                     VHARPCRPR"
     gene            complement(13845..15215)
                     /locus_tag="PK264_08000"
     CDS             complement(13845..15215)
                     /locus_tag="PK264_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75865.1"
                     /translation="MATDRQLIIEHVRALRERIAPLETALSASDAWLALTQLERRLRD
                     GSPLEAVDVRALKARLEGELALEPAFRSWRELTDTARELDTYLERREMPAIAWATIAA
                     RASGSTSAQGSAVPFRTRIRARLTQRENPLVARLLNKLRADGAMSPPAAPTSARSATA
                     SPIELAASRAAAIIARQQLETEAAREAAIASGRPDRGANDTILTPTRLTDRLAGAVAD
                     QTEPQPVSETDAETAELAEPNALPIGVAPPVQREPPPPLAPEDSQTRLARLETEVDAL
                     MLSVGGEADMPPMPLARRPIVSVGAATTEASRPAHPMVNTAAAPQRVAAPTAPDADPG
                     RIPPLLLDDIDEDDPAPDDEAQVMIVRRAASSDGSIGGAAGSALAVRRSRLTQRLNAM
                     LSGAAFNSDGYAAYRASVEEAAVVIVPPGGVPPPSQDTTKDGEPEDVGAVLRFLAVLN
                     GRSHRL"
     gene            15542..15931
                     /locus_tag="PK264_08005"
     CDS             15542..15931
                     /locus_tag="PK264_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75866.1"
                     /translation="MQIGKLDHVNVRTSNVAAMTAWYESVLGMKSGKRPPFPFPGAWL
                     YSGNDPTVHLVGVEARPENRDPQVEHFAFQAAGLKSFLERLAAGKIAHEVRKVPGFGI
                     VQVNVWDPDGNHIHIDFSPEEGLAAGM"
     gene            complement(15942..16418)
                     /locus_tag="PK264_08010"
     CDS             complement(15942..16418)
                     /locus_tag="PK264_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD75867.1"
                     /translation="MPIAFAIGVASVPMVGMAIGAGDVARARRTAWTAGILSGIIVGA
                     VGIVAVIAPNLWVALFTDSEAVKASTRTYLGLAGLGFPFMGFGLAIYFAAQGSGRILG
                     PVLAGSARLAVIAGGGYLLVKLGAGVSAYFALVGIAMLVYGIAMAIALWATPWQRN"
     gene            complement(16391..17311)
                     /locus_tag="PK264_08015"
     CDS             complement(16391..17311)
                     /locus_tag="PK264_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD75868.1"
                     /translation="MSGSAHPLLSGPVLPTLIKLSIPNIIALTTQSLVVIAETSYIGL
                     IGTEALAAMALVFPMIMLTQMMSSGAMGGGVSSAIARSLGAGNVARAETLAVHAVAIS
                     ICGGLLFMALFLTLGPWLLHVLGGRGRVLEEAMSYSRTFFFGVLAIWLFNTLVSVVRG
                     TGNMRLSASTTIAIALLQIALGGSLSLGLGPFPKLGIQGVALGQVLAFSAGVAFLVWL
                     LTSQNARVRLSPTGKSLDGEMFRDILKVGAVACLSPLQSVLTVLIMSGLVARLGTEAL
                     AGYRVIGLAWRKSSARGSSFCWCRSPSPSA"
     gene            complement(17342..>17811)
                     /locus_tag="PK264_08020"
     CDS             complement(17342..>17811)
                     /locus_tag="PK264_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRD75869.1"
                     /translation="DLLQELDRMEFYVYETGETVKARHRPIVMITSNNEKELPDAFLR
                     RCFFHFIKFPDKETMARIVEVHFPGLKGRLVSEAMKIFFDMREVPGIKKKPSTSELLD
                     WIKLLLNEDITPEMLREQDPRKLIPPLHGALLKNEQDVQLFERLAFMTRRKDN"
BASE COUNT         3174 a         5710 c         6002 g         2880 t
ORIGIN      
        1 acgaccttgt gcatcaacgc cgcaccgacg tgagcgagca cgaggagccc gagcccgatc
       61 gcggcccagc cgtgaagcca gagaacagtc ttcgccattc gctcatcctg ggcgaccagc
      121 gccggaagcg aatagccgcc gacaatctgc ggtgcgccat aggccgagac accgatccag
      181 ccgagcaccg gcaccgcgat gagcaatccg tagatggcga ggtgcgtcgc ctcggccgcg
      241 agccgctgca cgagattgag cgacggctcg ggcgctggca cgccgcgcgt gagccgcacg
      301 atcgcccgca acacgaccag ccacagcacc gcgaagccga tggtcttgtg ataggtgtag
      361 agctgcgccg tgaccgcgtc gaacttggtc gcaacgcccc gtcgcaccat gtaaagaccg
      421 acgccgatca tgccgaccac gagcagagcg atcagccagt ggagcgactt ctgcagcggt
      481 gaatagctcg cgggtcggat tcggctcatg gtgtcaggcc ctccacagct cttgaacgca
      541 gatcacaatc gaatagcacg tcgccgccgt cgagcacatg gacgtcggcg cggaacctca
      601 atgcctccga gagccgctcg atcggttgga gcgccagccc ggatttcccg gatcccagaa
      661 tgaccggcgc catcagaatg tgcaaccggt cgacggcgcc ggcattgacg aaggccgaaa
      721 cggtgtgagc accgccttcg atgaggatgc gatggaggcc tgtgcgggcg agcgctgcga
      781 ccacctcaag aggcgagatc acacctgccg ctgcagggag ttcgatcgtc tcaaccccat
      841 tgggataggg agacccgccg tcccggctac cgcacgccct gacgacgatg cgccgcgcac
      901 catcagcagc caggcacttg agatcggacg gcatccgacc gcttgggtcg aggatcacgc
      961 gggtcggatt gcgaccagtc acgtggcgca cgttgagctg gggattatcg gcgatggccg
     1021 tgccgacacc gacgaggacg ccatccacct ccgagcgcaa ccggtggacg tgaaccagag
     1081 ccgcatggcc atttatccag cggctttcgc cggttggcgt cgcgatgcgg ccatcgagcg
     1141 actggccgag ttgggcgaca acaaaggagc gcccggcgcc ggcctctcgc aacggactga
     1201 agcaatgctc atagtccatg attgtccgcc catgcttgga tcgtcggcct gcgcttacgc
     1261 tgcatcggca ggcccggtcc acaccgattt gcattcgata cgagcccgct gaatgagcac
     1321 ggggcccggc attgctgccg gcccccgcac cagtccccca gtcccaaacc agaaactcga
     1381 tcaggcgcgc acgatctgct tgatgcctgg aaccgtcggc accggctcca ccgttggccg
     1441 gtcgcggcga cggacctgag gcggctggaa ggcgcggcgc gcatggaatt cgcagtaggg
     1501 caggctcggc atcttgttct tgccgcagaa gtggaaatcc gccgcctgcg gatcgccgat
     1561 cggccagcgg cagctgcact cgacgagcgt ctgaatggtc ttgcgctcgg cgagcggaat
     1621 atcgagctcc tcgtgcgacg gcagcggctc cgcatccgct tcatagagct gccggatcag
     1681 cggatttccg agctgcgcga actttgcgcg ggcctggggc cgcttggcga tctggctgcc
     1741 gccgggagcc gcggtcttca cacgcggccg gatggccttg acgcgggact tcgtcgcgcg
     1801 gcctgcaagg ttgaggcgat gaactttgcc gatcacggca tttcgcgtga cgctgccgag
     1861 gcgtgtggcg atctggctgg cgctgaggcc ttccgcccac aacttcttga gcagctcgac
     1921 gcgctcgtcg gaccaggaca tggtgcttct cccaacaatc ggtgtcgagc gaagccaagc
     1981 ttgctcgtcc ccctactccg atcgaatcca cacgacgccg acgacattgc tgtccccgca
     2041 cggcgatcgc ggaacgccga ccagacccca agacacaact gaaggataac gcactacgaa
     2101 atcgcccgca gatgcgacgc gcggccgatt ccacgtgcac caggcaacag ggctcgttgc
     2161 ggtgctgcag cacatgctca gcgggcatgc gtgctcgtgg ttaacgaggg gaaaactact
     2221 cgccacggcg tccgacgaca agcgtggaga gcgacgacga ggggtattga ctgtcctgcg
     2281 ttgccatgcg gcaacaaatt gattcacagc caaattttcg ccatgctttg tccggtcaga
     2341 ccgccttgag agcgaacatc gtgcacggaa ggctgatatc gcctttgaac cggcaacttg
     2401 ccgcgtcagg cggcgaccgg gctcaacgcg acaaacgcag gacagccatc cgcgtcagcc
     2461 gggagggcaa tgtggacaac tgcgcaggtc ccgctgcact gataggtgcg acatcattcg
     2521 cggtacatgg catctgctgc cagatgcggt gacagaaagc tgtcagcctg caccagcgac
     2581 acgtcaacag ggcacacgag gtgcgcacaa tgtcagtctt tcgctccttc ctgttcgctc
     2641 ccggcaacca tccgcgccgc gtcgagaaag ccctgggcct ctcggccgac gccgtcattc
     2701 tcgatctcga ggacgcctgc ccgatcgccg agaaggtggc gacacgggcc gtagtagtca
     2761 ccgcatgcca gcgcgcgcgg agcgggctcg gctatatccg cgtcaacgcc atgtcgaccg
     2821 agtttggcta cggcgacatg gtcgccgtgg tgcagagtgg tgtcgacggc atcatcctgc
     2881 cgaagctcga gacggcggac gagatccgtg ccgcagactg ggtgatcgcc aatctggagc
     2941 gcgagcgcgg cttgaagtcc ggcgccttcg acatcatccc gatcatcgag accgcgcgcg
     3001 gcatggccaa cctgcgcgcc atcgcagctg cggggacgcg cgtgcgtcgc ttcgcgttcg
     3061 gcgccggcga tttcacgctc gacctcagca tggaatggac gcgggccgag acggagctgc
     3121 tcccctatcg cagcgagtgc gtgctcgccg cgcgggccgg caatctcgag gcgccaatcg
     3181 atacggtctg ggtcgacctc aaggatccgg agggcttcag ggcctcggca cgcctcgtaa
     3241 agggcttggg cttccagggg aagatgtgca tctacccgga tcaggtgccc gtcgtgaatg
     3301 aagtgttcac gccgacggcg gcggatgtcg aatggtcgcg acgcgtcgtc gccgccttca
     3361 aggaggccga gaaggccggc tcggcgtcga tccagctaga cggaaagttc attgattacc
     3421 cgatcgtcta tcgggcagag cgggtcatcc agatgatgga ccggatcgcc gcgcggcgct
     3481 gacggcctca cacgccgagc gcgcgcgcct tctcgatgag ggcagcgtca accgcgacgc
     3541 cgttcacgtc ggcccgggcc cgcgcggcct tgcggccagc gcccggcaag tgcgtcccag
     3601 gctcggcggc aatcgccgtg acgagatcgg cgatgcgcgc ttggaacgcg ccacccgaag
     3661 gggcgtcggg cgtgatcgcc atgaagcatt gccccgtctt cggcgggccg cccgcggttc
     3721 cggagaacgg gctcgccgcg atgccgagcg tggcgcctga gaggcacgct gccatcacct
     3781 ccaccaggat cgccgagctg acgcccttat aaccacccgc aggcatcatg gtgcccttga
     3841 gcccgacgtt cggatcggtc gtgggctgac cgtcaggtcc cagcgcccag ccctgcggaa
     3901 tcggctggcc cgaccgcgcc ttcttcatca cctcgctctt ggcgacgacg ctggcgctct
     3961 ggtcgatggc aatcgcgacg ccgcccttgc cgtccggcac cgcgagcgac caggggttgg
     4021 tgccgacgac ggccttccgg ccgcccgacg gcgcgatcga agccggcgca ttggtgaagc
     4081 cgagagcaac gaaccccgcc tcggcgagtc gttcggtatg gtggcccaga acgccgcagt
     4141 tgtaggagtt tcggatgacg agcgtcgcga tgccttgcgc ctttgcgagc gggatcagcc
     4201 tcgcgaagcc cgcgtcgatg gccggatggg cgaagccgga ggcggcatcg actgccacca
     4261 ctgctggcgc tggcgttgtc accgccggga cggcgctcgc aagcaccttc ccgcactgga
     4321 gatgctcgca gtaggtcgcg aggtaggcga ggccgtgcga ggcgatccca tcccgctctg
     4381 cagccgcgat cgccttcgcc atgctgcgcg ccacctccgg ccgggcgccc gctttcgaga
     4441 gcgcccgctc ggcgatcgag aaaatctcgc ggaccgtgat cgtgccaggc atgaggcgct
     4501 cctcgtgacg aagatgacga cggaacgata cccgggagtt ctccaaatgt cgcgggagtt
     4561 ggtgaccatg accaatcaca tcgcagacgc gcggtgagcc ctacgataag ctcgcgatgc
     4621 ccaatctcac cggcggagga cagcaatgcc cgcgctccat cgcgactaca atctggccga
     4681 tggcgatgcg atctatgccg agctggtcgc ggcgcatcgt ggcctcgacg agcgccagag
     4741 tgccgccctc aacgcccgcc tggtgctgat cctcgccaat catatcggcg atacggccgt
     4801 gctccgcgag gcgatcgcgg ccgctcttgc gagcgcttcg agttcccccg gctgatcacg
     4861 gccggttgcg gatcttctcg agtacgggga ggatgtagga acggaagtgc gccgggttct
     4921 cgctcatcgg gaaatggccg agccgctcca tgatggtgac ctcgactccg gcgatcgcct
     4981 cggcggtccg ccgcgtatcg tcgggcgtgc aggagaagtc gtactcgccc gtgagcaggt
     5041 agagcgggca gcgacgcgtg tcgattttgg cgatcttggc gcgcaggtcg ccgtcgacgc
     5101 gatagaagta gaggtcgccc ttgaagacgc cgggtcccga cgatttgtac atccagagcg
     5161 tctcgtgccg gctctcggcg gggctttgag gggccatcat gccggagaca aggccggcgc
     5221 acacctcgcc gccgtgcacg tcgggacgat ggagccaatc ggtgtcgtac cagggttgct
     5281 ggtgcgccgc ggattcgagc ccgatcagcg cgcggaagcg ctcgggatgc tcggcggcga
     5341 gattgagcac gatgcgcccg ccgatcgagc agcccatgat cacgggccgg gcgagttcga
     5401 gcgcgctgca gaggctcaag atccactcgg tgtaggcggc cgtcgtcagc cggtactcct
     5461 ctcgctcatg gccgacgggc ggcagcgact tgccgtgcca gggcatgtcc ggcaccagca
     5521 cgcggtagtg cgccgtgatc gcgggatcgt tgnnnnnnnn nnctccagca gcatggcgat
     5581 gccatcccag cgcgggtcgt tgaggatgtg gcggaactgg cggccgtcgg caccggctgt
     5641 atgcaggcag aggagcggaa taccctggcc cgcctcctcg acgtaaacgc ggtgcgccct
     5701 ggcgccgagc tcgagggaga aatagcgccc ggtgatgggc tcgaagcgcg gtgccatgtg
     5761 tcagctcttg tgcgtcccga ccatgccgcg gggcagcgcg aggatgtcct tcacgacctg
     5821 caggctcgcc atgaagggct gcaggtttcc ctcgatccgg agggcgccgc gcttcgagag
     5881 ggccaggatg tcgtggctgc cggggggcgg tggatctgaa aggaacgcct gccagacctc
     5941 cggctcggcg cggatggcaa tcgtccaggg ccggaggagg aacgggccac gcagaacgtc
     6001 cttcacgtcg ccgccgacga tccgggcaat cagagtctcg gagccgacac ctatggcgac
     6061 gtcgagggtc gcatggcgtc cgcgccgggc gatgtcggca tcggcggcgg caagctcggg
     6121 caggcggctg aaggcggcaa cgactgcgga ggtcatcacg gcgcgaccgg ggcgagcgaa
     6181 aacacgacaa gcgctgctcc gaggcagccg agcaccgctc ctgccgcccg cacgcgcgga
     6241 tgatgctggc cgagcgtacc tgtcatgacc ttggcaaagg cttgcgcagc agcagcgatc
     6301 gcaaaggata cgacggcaat gccggcgaca taggccgtga ttacgccctg ctcggagccg
     6361 agtaccgact cggcgagcgc atagccatgc acgaggccgc cgttgaaggc gagaaacgac
     6421 cagaacgagc cggccaattg tagcccggtg aagagcagca tcccgaccgc gaggacggag
     6481 agggcgacca gcagctcgac catcggcagc ggcatgccct tggcatgtgc cagcacacca
     6541 agcgccgagc tgatgacaaa acccatcggc acgacgagac cgccgcgcac aaaggcgctc
     6601 gcaaagccaa tcgccacgat gaaggccagg tgatcgggcc cgatcaccgg atgcccgatg
     6661 ccggagagca gcccctgcag gatggtctca ggccttttgc ctcccattgg gtgatgcgcc
     6721 agcgcgggaa ccggcaggag aagtgctgcc atgagcagcg cggcgcacac agcttgcgcc
     6781 cgtcgaagga ttgtcggcac cggatcaccc tgagttaagg tcagttgaca ggccgcatca
     6841 tgcaacgccg gcgtcgcgag cggaagaggc agtcggctca ccccttgcgc aggtggcatg
     6901 caacccagtg accattcgcg ccctgctcga gcttcggcgc ttcggtgctg cagagtggaa
     6961 gctgtcggat cgggcagcgg gtgtggaagt ggcagccgga tggcgggcgg atcgggttcg
     7021 gcacatcgcc ctcgagcatg atgcggcggc gcttcacctg cgggtcgggg atcggcaccg
     7081 cggagagcag cgcctctgtg taggggtgct tcggcgccgc gtagagatcg tgcgcagatg
     7141 cgatctcgac gatacggccg aggtacatca cggcgacgcg ctggctgatg tgctcgacga
     7201 cggagagatc gtgcgcgatg aagatgtacg tgaggttgaa cttctcctgc aggtcctcga
     7261 gcaggttgat cacctgcgcc tggatcgaca cgtcgagcgc ggagaccggc tcgtcgcaga
     7321 cgatgagctt tggttcgact gcgagtgccc gcgcgatgcc gattcgctgg cgctggccgc
     7381 cggagaactc gtgcgggtag cggcgcatgt gatcgggctg caagccgacg gtgcgcagaa
     7441 gctcggcgac gcgctcctca tgctccttct tcgacttggc gaggccgtgg atcaccagcg
     7501 cctcgccgat gatcgcgccg accgtcatgc gcggattgag cgaggcgaac gggtcctgga
     7561 agatgatttg catgtcgcga cagagtgcgc ggagctgctc ccggcccatc tccgagacgc
     7621 tcttcccctc gaaccagact tcacccgcgg tcggctcgat gaggcggagg atgcagcggc
     7681 ccgtcgtcga cttgccgcag cctgattcgc cgacgaggcc gagcgtctca cccttggcta
     7741 cctcgaagga gacgccgtcg acggcgcgca cgcgatcgac ctcgcgctgc agaagcccgc
     7801 cccggatggg gaagtgcttc acgagttcat tcaccttgag cagcggtgcg gacatcttga
     7861 agacctctcg ttcgcaccac tagaggatgc aggcgacctt atgaccgggc tcgacctcgc
     7921 gcagctgcgg ctcggcggcc cggcaggcat ccgtcgcgaa attgcagcgc ggcgcaaagc
     7981 ggcagccggg cggcggccga agcaggctcg gcaccacgcc cttgatggct tcgaggcgtg
     8041 ccttgtgcgt cgcggcgcgg tcgattcgcg ggatcgagcg gatgaggcct tgcgtatagg
     8101 ggtggcgagg attgccgaag agcttctcga ccggcgcttc ctcgatcacc ttgccggcgt
     8161 acatgacgac gacgcgctgc gcggtctcgg cgacgacgcc catggcgtgc gtgatcagca
     8221 tgatggccat gccgaatcgc gatttcatgt cctggagtag ctcgaggatc tgcgcctgga
     8281 tggtgacgtc gagcgcggtc gtcggctcgt cggcgatgag taactgcggc ttgcaggaga
     8341 gcgccatggc gatcatcacg cgctggcgca tgccgcccga gaactggtgg ggatagtcgg
     8401 tgacacgccg ctccgggtta gggatctgca cgagacgcag catctcgatc gtacgcgcga
     8461 gcgcctcgcg cttggacacc ttctcatgct ggcggatgac ctccgcgatc tggtcgccga
     8521 ccgagtagac cgggttcagc gaggtcatcg gctcctggaa gacgatggca atctcgcgcg
     8581 ccctgacctc gttcatctca gccttgtcga gcggcacgag atcgcggccc tgccagagga
     8641 tctggccggc gacgatctgg ccgggcggca tggcgattag cttcaggacc gacatcgccg
     8701 tgaccgtctt gccgcagccg gcttcgccga cgacgctcac ggtctcgccg ctgtggatgg
     8761 tgatgtccac gccgtcgacc gcgcgcacca tgccgtcgtc ggtcttgaag tgcgtcttga
     8821 gaccgcggat ctcgagcagc ggctcgcgca ctccgttcgc cgtacccgcc gccggcctgg
     8881 tcgccacatc agtcattggt gcacggcctc gagcttgtgt ccgtcgggat cgatgacgaa
     8941 ggcggcgtag taggtcgggc tgtagcgcgg ccgcggcccg ggcgggccgt cgtcgcggcc
     9001 gccatgggcg agtgcagctg cggagaaggc gtcgaccgcg tcgcgggacg gcgcaacaag
     9061 cgccaggtgg aagcccgggc cgggtgcgcg ggcagctccg ggtcgatgaa agagcgcgag
     9121 cctatcagga ccgccgagcg cgccgaaccc ggctgcagtg tcagtcgtcc aaacgcgtgt
     9181 gacgccgagc ggcgcaaggg ccgcatcata gaatcggatg gatcgcgcca ggtcctcgac
     9241 gccgaacgag atgtgaccga gcatcgatgc accgctagca ctacatcacc ttccgcggat
     9301 cgagagcgtc gcgcagtccg tcaccgatga agttgatgga gagcacggtg aggaagatcg
     9361 ctgttcccgg gaagagtgcc cagtggggcg cgaaatcgag gttatcgcgg gcatcgaaga
     9421 ggatgcgccc ccaggtcggg atatcgggcg ggaagccgag cccgaggaag gagagcgtcg
     9481 actcgatgat gatggcgagc gcaacgtcga tcgtgccggc gacgatgacg gggccgagcg
     9541 cgttcggcag gatgtggcgt acgacctgac gcgtcttcga ggcgcccagc gcacgcgcag
     9601 cctcgacgaa ctccttctcg cgcagcgaga ggaactgcgc acgcacaagc cgcgcgaccg
     9661 gcatccagcg gagcccgccg atgacgatga cgatcaagat aaagatgccg ccttccggcc
     9721 cgaagacatc cttcaaggcg tcgcggaaga ggtagatgac aaggaggagc agcggcagct
     9781 gcgggagcga gagaaagagg tcggtcaacc acatgagggc ggtgccgacc cagcccctcg
     9841 acatgccggc tatggcgccg acaatggttc cgacaacgac cgcgacgatc atcgccgcga
     9901 gacctaccgc gagcgagatg cgcccgccat agagcatgcg ggcgaggatg tcctggccga
     9961 ggtcgtcggt gccgaaggga tgcgcccagg acggcccctg cagcttggcc ttgaagtcga
    10021 tctcgttgat cggcgtcttc cagacgtagg gtccgatcag cacggcgagg atgagcagcg
    10081 tcagcacgat cgtgccgacc acggcgaggc gatgacggcg aaatttccgc catgcctccc
    10141 gtgccggcga atagatcggt gctgcctgag caactgccgc agtcgccggc tccggcgcat
    10201 ttcgcggcga atacgtcggc ggtggctggt cgaccacact catcgggagg tctcctgatg
    10261 gcgtgaaagt ttgccgcgcc aacagccgag acttgcgatc aaactgtgtt ccacgacgtg
    10321 cgcctcagct gtaggagatg cgggggtcga gacagccgta aaggatgtcg gcgatcaggt
    10381 tgaagaggac gacgaggcag gcgaagatga aggtcaccgc catgacgacg ggtgtgtcgt
    10441 tggcgaggat cgagctgatc agcagcgagc cgatgcccgg gatgcgaaag atctgctcag
    10501 tcacgatggc accgccgaag attgcgggtg cctggagcgc cagcagtgtc acgaccggga
    10561 tgagcgcatt gcgaacgacg tgcttcagga tgaccgtgcg ctccttgagg cccttcgatc
    10621 gggccgtcgt cacgtagtcg aggcggatga catcaagcac ggaggaacgc acgtagcggg
    10681 tgagcgaggc cgcctggaaa agcccgagaa ccgcaatcgg catgatcgac tgcttgaagt
    10741 gctcccagaa ccattgccag ccggtggccg cgatgtccgc gcggtagacg aacggcagcc
    10801 agccgagcgt gatagagaac agcaggatga agagaatgcc ggtgaagaac gtcggcagcg
    10861 agaagccgat gaaggcgaag gtgttggcga gctggtcgaa cagcgagtag gggcgggtcg
    10921 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg agcacctgcg ccgagccgat
    10981 gacgaagagg gtcgtcggca gcgcctgaag aatgagtttg tcgacatcca tgcggctgac
    11041 gaacgagaag ccccagttgc cctggaacat cgccgtgatc caactgaagt agcggagatg
    11101 gatcggatca tccaagccga acttcgcccg aagtgctgca cgcacctcgg gcggaacggc
    11161 cgggttggtc gcgagctcac cgaacggatc gcccggggcc agcgccagca ccgtgaagag
    11221 cacgacactg atcccgagca ggctcggcac cgcgatcagg aggcgcctca ggatatacat
    11281 tcccatcgaa cgcggtcccg atcaggcagt ggccgaagct ttgcggcgag aggtcgatct
    11341 gcgtcggctc atgcctcgcg gtaccacgcg gcgatgttcc acgtggtgtt gtcccagccg
    11401 ctcaagtgcg gccgcagctt gagcgaggcg ccaccggcct gtgggcgagc aacgacaggg
    11461 atcacggcac gtgcagcgat tacgaggtcg ttgcacttga tgaagagcgc cgcgcgcttc
    11521 acgggatcga gttcagcgac gccggccttg taggtgtcgt cgtactcctt gctcgtccag
    11581 cgcgtgatat tgcggccctg ccacttgttg gccttggtgg cgacctccca ggagcagaac
    11641 tggttcatga acagctcggg atcgggctgc gtcatggtcg tcgtgtacat ctgcaggtcg
    11701 cagaagaact tggtgtaggt gtccggattg gcgacgtccg acgagaagta gaccgacgcg
    11761 gtcaccgact tgagctcgat gtcgatgccg gccttctgcg cggcctgctt gacgatctgc
    11821 tggttcttct ggcgcggcgc gttgatggac gtctggtaga cgaacttgag cttcttgccg
    11881 tccttggcgc ggataccgtc ggggccgggc ttccagcccg catcgtcgag taccttgatc
    11941 gccttttcga tgttgaactc gaacttggtg ttcttcgagg tgaacttgtc gggattgttg
    12001 acgaagttcg ccgtcgcgat gccagtgcgg ccgtagatgt gatcctgcac cgacttgcgg
    12061 tcgacgagca gattgagcgc ctcgcgcacc gccggatcgg caagcgtcgg gtgcttcgac
    12121 ttcatgctcg cccgctcgcc ctcgacctcg ttccagggat cggtgaaatt cacctggatg
    12181 tgctcgatgt tgcctgtcgg cacgatgctg acggcaccct tgccgccctt ctcgaggcgg
    12241 agcagtatct cgtcctcgac ctgcaggttc caggcgtagt cgtactcacc ggtctggagc
    12301 accgctcggg ctgccgagac ggcgtcgccg ccgcccttca tttccacggc gtcgaagtgc
    12361 ggctgattcg ccacgtggta gtttgcatag gcagcgccct tgaccatgtc gccgggcttg
    12421 aagtcgacga aggtgtaggg gcccgtgccg acaggcttaa ggtttgccgg cgcctcgcga
    12481 gacttcgctc ccttgtagtc cgcgaagaga tgcttcggga tgatcatgcc gcgcgtgccg
    12541 acgaaggcat cggcccagaa tggcgtcggc ttggcgaaca cgacgcgcac cgaggtgtcg
    12601 tcgatcttct cgaccttcac gtccttgtag ctgccgatcg tcaccgcggc cgtgccgggg
    12661 tcagtagcgt actcccagtt gaagactaga tcgtcggccg tcagcggttt gccgtcgtgc
    12721 caggtgacgc ccttcttcaa cttccagatc accgtcttgc catcggccga gaggccgcca
    12781 ttggccgtgc tcggaatctc ggctgcaagg atcggcacga gattgccgtc ggcatcccag
    12841 gcggcgagcg gctcatagaa caggcgcgag ccgtcctggt ccttggtgcc gaccgcgaaa
    12901 tgcggattga gcagcgtcgg gccttgccac cagaggagct tcagcgtgcc gccgccgccg
    12961 cgcttggtcg gcttgtaggc gaacttcgac tgcgccatgg cgactccgcc gagcgccagg
    13021 agctggttgg ccagcggcgc ggcaaggccg gcggccgcca tgcgcgtcag gaacgagcgc
    13081 cgcgacagcg acccttcttt cacgcgatcg atgaggcgat gaaggctttt gggttccatg
    13141 ctcagtccct ccctggttcc ggacgtcgat acagcgccgc tgctcctgcg gaccgaagtt
    13201 acggagcggc cctgcaagtc ggcactacag tcatgttggc gacgcagctt aagcaacgcc
    13261 gcgaggtcac aacgaggctc tcctgcatgc gcctcggcgt gagcctatca aaacaggcgc
    13321 ggggcgcgtg gtcaatgatc gggcagatac caatgcggcc tgcccggcgc gcagtcagtg
    13381 ttaccgaacg gtcagcccat cggaaaatgt cggcctcgcg gcatcctgtc caagcatttc
    13441 gcgacacaaa ccgaggcaca ctccgaatca gcgctatgca ccctcaatcc cgcgcgcagg
    13501 aggaccatat gaaaattcga catgccattg gaatcgcgtt gctttccgtc cctttcctgg
    13561 cagcttccgc gagtgcccag ggcatgtccg atctccatac ccaggttcgc atcggcaacc
    13621 gcatctgcat ggccgaccat ttccataccg gctccggcaa cggtgcgtcg aaggacgcgg
    13681 ccatgcgcgc ggccatctcg agctggtctg acttcacggc gtgggagtac ggcaacaact
    13741 ggggctcatg gcggcttgcc gagacgaagt cgtccacttg cgagcccggg ttcggcggcg
    13801 gcttcagctg cacggtgcat gcacgcccct gccggccccg ctgatcagag gcggtgggag
    13861 cgcccgttga gcacggcgag aaacctgagg acggcgccga cgtcttcagg ctcgccgtcc
    13921 ttggtggtgt cctgcgatgg tggcggcacg cctccaggtg gcacgatgac gacggccgcc
    13981 tcctcgacgg atgcgcgata ggcggcatag ccgtcactgt tgaaggccgc gccactcagc
    14041 atggcgttga ggcgctgggt cagccttgag cgccgcaccg caagcgccga tcccgccgcc
    14101 ccgccaatgc tgccatccga ggaagcggca cgccgcacga tcatgacctg ggcctcgtcg
    14161 tctggcgccg ggtcgtcctc gtcgatgtcg tcgaggagaa gcggcgggat ccgcccgggg
    14221 tcagcgtcgg gcgccgttgg cgcggccact ctttgcggtg cggcggcggt gttgaccatg
    14281 ggatgcgcgg gccgcgacgc ctcggtcgtc gccgcgccga cgctgacgat cggacgtcgc
    14341 gcgagcggca tgggcggcat gtccgcttcg ccgccgacgc tcagcatcaa ggcatcgacc
    14401 tcggtctcga gcctcgcgag cctcgtctgg ctgtcttcgg gagccagcgg aggcggcggc
    14461 tcgcgctgga ccggcggcgc cactccgatc ggcaaagcgt tcggttcggc cagctctgcg
    14521 gtctcggcat cggtctccga cacaggctgc ggctcggtct gatcggcaac cgctccggcg
    14581 agacgatcgg tcaaccgggt cggggtcagg atcgtgtcgt tggcgccgcg atcaggccgg
    14641 cctgaagcaa ttgccgcttc acgggccgcc tccgtttcga gctgctggcg ggcaatgatc
    14701 gccgccgcgc gcgaggcagc caactcgatc ggcgaggcgg tagcgcttct cgccgatgtc
    14761 ggggccgcgg gcgggctcat cgctccgtcg gcgcgcagct tattcagcaa gcgcgcgacc
    14821 agtgggtttt cgcgctgtgt cagccgcgcc ctgatgcgcg tgcgaaatgg caccgccgag
    14881 ccctgagccg aggtgctccc ggatgcgcgc gccgcaatcg tggcccaggc gatcgccggc
    14941 atttcacgcc gttcgagata ggtatcgagc tcgcgagccg tatcggtgag ctcacgccag
    15001 gatcggaatg cgggctcgag cgcgagctcg ccctcgaggc gcgccttcag ggcccggacg
    15061 tcgacggcct caagcgggct cccgtcgcgg agccgtcgct cgagctgcgt gagcgccagc
    15121 cacgcatcgc tggccgacaa tgcggtctcg agcggggcga tccgttcgcg cagcgcccgc
    15181 acgtgttcga tgatcagctg gcggtccgtc gccatgtcga tgtccgaaac cccaactcga
    15241 cgcccggtcc cgaatccggc ccgaaattcg gctcagaagc gagccggtca tctggagtga
    15301 cgtgtcacgc tcgcggcccg cgtctgcgac gaccgtcaac gtacactcac tccaatgccg
    15361 tgtgcacatc cgcccgctac cggcactatg ccggactgca ccgaactata ccacgccaaa
    15421 tcagcgcctt gcgatacgct tgccgctccg cgtggcgcaa tcgcgacggt gctgtctctt
    15481 cacgcgcgct tatggcaagg tctgagccgc atttcgcctc gacggccggc aggagagccc
    15541 catgcagata ggcaagcttg atcacgtcaa cgttcgcacg tccaacgttg ccgccatgac
    15601 ggcctggtat gagagtgtgc tcggcatgaa atcagggaag cgaccaccgt tcccgttccc
    15661 gggcgcctgg ctctattcag gcaatgatcc gacggtgcat ctcgtcggcg tcgaggcgcg
    15721 gcccgagaac cgcgatcccc aggtcgagca cttcgccttt caggccgcgg ggctcaagtc
    15781 gttcctggag cggcttgccg ccggcaaaat cgcccacgag gtgcgcaagg tgccgggctt
    15841 cggcatcgtc caggtgaacg tgtgggatcc ggacggcaac cacatccaca tcgacttctc
    15901 gcccgaggaa ggcctcgccg ccggcatgta ggcgcggccg atcaattgcg ctgccagggc
    15961 gtcgcccaga gcgcgatcgc catggcgatg ccgtagacga gcatggcgat gccgacgagc
    16021 gcaaaatagg ccgacacacc tgcgccgagc ttgacgagca gatagccgcc gcccgcgatt
    16081 accgcgagac gcgcagagcc ggcaagcacc ggcccgagga tgcggcccga gccctgcgcc
    16141 gcgaagtaga tggcgaggcc gaaccccatg aacggaaagc cgagaccggc gagcccgaga
    16201 taggtacgcg tcgaggcttt caccgcctca ctgtcggtga acagtgccac ccagaggttc
    16261 ggcgcgatca cggcgacgat gccgacggcg ccgacgatga tccccgagag gatgccggcg
    16321 gtccacgccg tccgccgcgc gcgcgcaacg tccccggcac cgatcgccat gccgaccatg
    16381 ggcaccgagg ctacgccgat ggcgaaggcg atcggcacca gcagaaactc gagccgcgcg
    16441 ccgacgactt gcgccacgcc aatccgatga cacgatagcc cgcgagcgcc tcggtgccga
    16501 gccgcgcgac cagccccgac ataatgagca ccgtcagcac cgattgcagc ggcgagaggc
    16561 aggcgaccgc gccgaccttc aggatatcgc ggaacatctc gccatcgagc gattttccag
    16621 tgggcgagag gcgcacacgc gcattctgag acgtgaggag ccagacgaga aaggcgacgc
    16681 ccgccgagaa ggcgagcacc tgcccgagcg ccacaccctg gatgccgagc ttcggaaacg
    16741 gcccgagccc gagcgacagt gagccgccga gcgcgatctg caggagcgcg atcgcgatcg
    16801 tggtcgaggc tgaaaggcgc atgttgcccg ttccacgcac gaccgagacg agcgtgttga
    16861 agagccagat cgcgagcacg ccgaaaaaga acgtccgcga gtagctcatc gcctcctcga
    16921 gaacgcgacc gcggccgccg agcacatgga ggagccacgg cccgagcgtc aggaagagcg
    16981 ccatgaacag gagcccgccg cagatgctga tcgcgacggc atgcacggcg agcgtctcgg
    17041 cgcgggccac attgccggcg ccgagcgagc gcgcaatcgc cgatgagacg ccgccgccca
    17101 tggcgcccga cgacatcatc tgagtcagca tgatcatcgg aaagaccagc gccattgcgg
    17161 cgagcgcctc ggtgccgatg agtccgatgt aggacgtctc cgcgatcacc accagcgact
    17221 gcgtggtgag ggcgatgata ttcgggatcg acagcttgat gagcgtcggc aggactgggc
    17281 ctgatagcag cggatgcgca cttcccgaca ctggagcgct tggccgtccc ccatccgaca
    17341 ctcagttgtc ctttcgacgc gtcatgaagg cgagccgctc gaagagctgg acgtcctgct
    17401 cgttcttgag gagcgcgccg tgcagcggcg ggatgagctt tcgcggatcc tgctcgcgca
    17461 gcatctcggg cgtgatgtcc tcgttgagaa ggagcttgat ccagtcgagc agctccgagg
    17521 tcgacggctt cttctttatg ccgggcacct cgcgcatgtc gaagaagatc ttcatcgcct
    17581 cgctgacgag gcggcccttc aggcccggga agtgcacctc gacgatgcgc gccatcgtct
    17641 ccttgtcggg gaacttgatg aaatggaaga agcagcggcg caggaaggcg tcgggcagct
    17701 ccttctcgtt gttcgacgtg atcatgacga tcgggcgatg acgcgccttc accgtctcgc
    17761 cggtctcgta gacgtagaat tccatgcgat cgagctcctg caggaggtcg t
//
LOCUS       DAORNH010000057        17554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2451_length_17554_cov_2.686325, whole genome shotgun sequence.
ACCESSION   DAORNH010000057 DAORNH010000000
VERSION     DAORNH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17554
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2451_length_17554_cov_2.686325"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="PK264_08025"
     CDS             complement(<1..1384)
                     /locus_tag="PK264_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD75870.1"
                     /translation="MATFSQSLEQALHRALEYANQRHHEFATLEHLLLALIDDRDAAA
                     VMRACNVDLEVLRKQITEYLDQEQSSLVVKGSAEATPTNSFKRVIHRAVVHVQASGRD
                     EVTGANMLVAIFAERESHAAFFLQEQDMTRFDAIQYISHGIAKRAGLSEPRPVRGVDE
                     ESQGSDGVGEKKNSADALSTYCVDLNKKAAEGKIDPLIGREAEVMRTIQVLCRRQKNN
                     PLFVGDPGVGKTAIAEGLARKIIKGEVPEVLRGATIFALDMGTLLAGTRYRGDFEERL
                     KAVIKEVEQHPGAILFIDEIHTVIGAGATSGGSMDASNLLKPALQSGAVRCIGSTTYK
                     EYRQHFEKDRALVRRFQKIDVKEPSIEDTIEILKGLKPYFEEYHKLRYSNDAVKAAVE
                     LSAKYIHDRKLPDKAIDVIDETGASQMLLPEGKRKKKIGVKEVEATVATMARIPPKTV
                     SKSDAEVLGNL"
     gene            complement(1391..>1639)
                     /gene="clpS"
                     /locus_tag="PK264_08030"
     CDS             complement(1391..>1639)
                     /gene="clpS"
                     /locus_tag="PK264_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HRD75871.1"
                     /translation="PSLYKVLLLNDDYTPMEFVIHVLERFFSKGREDATRIMLHVHHK
                     GVGICGVYTFEVAETKVTQVMDFARQHQHPLRCTMEKE"
     assembly_gap    1640..1715
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2024..2923)
                     /locus_tag="PK264_08035"
     CDS             complement(2024..2923)
                     /locus_tag="PK264_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD75872.1"
                     /translation="MAAEHGSELKAALAAAIGSICVGFTPLFFSGLHNAGLETVTILF
                     YRYLVALCVLVPIALSQVPSIAEEWRRGGAHLFLNGLLLGSAQTFFYFKAVETLPTSV
                     VVTFFYAYPLVTILIDRFFFKLPVYGTTLLAAAVVIVGVAMTSLPGLKDANLDPVGLA
                     FACASPLGYALYIAVAYPATRRVAPFASAMFIYGSLAVAFGSIALSRGLKLPPTGALW
                     LSVLFIGTLGGALQILAFSYALPRLSSSGYALIVSLELVTVVLAGVLVLGEPLQLIQG
                     VGIALVLAGVLFERLMRARRAPS"
     gene            complement(2926..3717)
                     /locus_tag="PK264_08040"
     CDS             complement(2926..3717)
                     /locus_tag="PK264_08040"
                     /inference="COORDINATES: protein motif:HMM:NF004795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD75873.1"
                     /translation="MSDQPPTIATKFKDHHGSRRVARVTVDNQRRLNCLTSAQNLLLA
                     DTFHALSRDETLACVVLTGAGDTAFIGGADLKELGAADHDSGRLYITRLHQACKAIRD
                     CPVPVIGRINGFTLGAGLEIAASCDFRVAAAGAVLGMPEVHMGLPSVIEAALLPGLIG
                     WGKTREILMTGETFDAEAALAMGLVEKVVPASGLDAAVDHWIGGILKATPKAIRAQKA
                     LIDHWQRSSVEEGVLAGIEALSNSYRTGEPQAAIARFFTSKKKKG"
     gene            3872..4414
                     /locus_tag="PK264_08045"
     CDS             3872..4414
                     /locus_tag="PK264_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75874.1"
                     /translation="MSAASSISGKWPALLRVFMLLIFLGAPLVLVRDAQDLWHDFASR
                     QHTFVPAPNVRIVEAKCSIGGRQCTIKVDGGVSVSGRAEFKYAFFESMTGKTVVPVRS
                     SADGSITIKEGRERLGQRLAMLIFAALLIIALNLGLIASFVTADRLRPYLDAAARTSL
                     LASLAAFFGGLILYAAGVIA"
     gene            4471..5124
                     /locus_tag="PK264_08050"
     CDS             4471..5124
                     /locus_tag="PK264_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75875.1"
                     /translation="MPLQNRVLPTGEIVASPARGLMMGNRGGRFHRADRTLGPRRWAS
                     RAWIACRLNFKGRHRPAMMMANSYTELFFLDEATALAAGHRPCFECRRADADCFARLW
                     AGLRGGKERASADEMDRALHAERLAGPSGPDRWRALRDVPDGTIVEQDGPCLVLAGML
                     LPWAITGYRPPLSGRPAAVRLITPPAIVAVISAGYPPMVHPSAHPAAASMAVRSTLD"
     gene            5132..5974
                     /locus_tag="PK264_08055"
     CDS             5132..5974
                     /locus_tag="PK264_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75876.1"
                     /translation="MTRSTLRRSMRRVYWFLGLIILAAVVAKFAEHVPGLKDTLILGV
                     FKDIYELIRDMALLIATGGVAAITNTYQRRSQFIEALRQEWREIVQAKSAVFTYTQLE
                     NPTTEQYLATFCKLSETIDNMRTVYANVGETGDLVGLYPYAPLHDMRRALQTLDPRLG
                     GDLSPAARKKVRDATLQVFYAVRDRFLEELDLEEPDAPVLISGARRLKSSGAAGYAGR
                     MQGREAKGVAKAPGGSAEIDQFLAELKAKEDATSKIWRPAAGNGAVSAPGRAAPPSNS
                     RPAS"
     gene            complement(5957..6490)
                     /locus_tag="PK264_08060"
     CDS             complement(5957..6490)
                     /locus_tag="PK264_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRD75877.1"
                     /translation="MFERNKVDNSLEQAAIGVEITMDGGEVLSGKLAIPMSRSLFEVL
                     NSQVAFLDFEPYDGDRRFLAKTAVKAVRLVGGTKAASLESRLIDLDGFDPWRVLGVPR
                     GSSFDIVRQAFHAKAKTYHPDRYASADLPAEVRDYLAAQVRRINEAHRVLEQGQQAEK
                     RATAARAEPIYTSARRA"
     assembly_gap    6677..6920
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6921..7558
                     /locus_tag="PK264_08065"
     CDS             <6921..7558
                     /locus_tag="PK264_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRD75878.1"
                     /translation="SLSPSSFSAGYAEQPTASVPAARKSGRSAVGATSDTVPGLPAPP
                     TSTPPVTAKTRTADLAPPGSFDVAPRGALSGRDYSLTALDVERARDLINAYRRQNGLK
                     PLTLHPKLTEAAKLHARDLAKWDRISHFGSDGSNPWDRVKRSGYNARLAAENVGTGQV
                     TFEEVLKGWKDSPGHNKNLLLADADHIGLALVQDPKTEFKTFWTLVLGAPL"
     gene            complement(7599..8993)
                     /locus_tag="PK264_08070"
     CDS             complement(7599..8993)
                     /locus_tag="PK264_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HRD75879.1"
                     /translation="MKADPTAAPALAEPIHDPIGARTLLGALLRGSSDRNRAGRDAIL
                     AFLVRAVSAALLYLSQVILARWMGSFEYGVYVFVWTSVLMLGGVSTLGFGMTMIRLLP
                     TYRERGERHLFDGLLRAGRLIPLAAATLIAGLGLLAIWLAGERLSGPYVLSAFIVLFC
                     LPLFTLSEVHDGIGRGSGWLAAGLLPHYVIRPLLVLACMGLARLSGLAMDAKTAAGAA
                     VVATWLAAVLQLALIERKLADEPRADVRRYDIAGWFRIALPLLVIATCELVLQNADLL
                     IVSRYLSPSDVAIYFAAAKTMSLILFVHYAVGSAVANRYAALDVRGDRQALEGLVRDA
                     VNWTFWPSLLGAGLLLALGKPLLWLFGPQFTSGYAVMAILALGFLARSIVGPAEFLLN
                     MLGEQRRCALILVLAAVVDIALGLLLVPSLGLIGGAIATSTALVAAAFAQWLSARRRL
                     GLRIAIWENLRARP"
     gene            complement(9062..9301)
                     /locus_tag="PK264_08075"
     CDS             complement(9062..9301)
                     /locus_tag="PK264_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75880.1"
                     /translation="MMRHTTAAPWHPEMDTTPTPREVDMMASVLEGRHGVYAAEVAEF
                     FAAAHGDHGDAGRCWAWAGVAEIIRRRERARMTSS"
     gene            complement(9559..9831)
                     /locus_tag="PK264_08080"
     CDS             complement(9559..9831)
                     /locus_tag="PK264_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRD75881.1"
                     /translation="MSKKDLIDAVAKEAELTKDKAATVVDAVIKHIGEVMKKGEEVRI
                     PGFGTFKVSKRAARTGKNPLTKEPIKIPASNVPKFQAAKALKELLN"
     gene            complement(10684..11262)
                     /locus_tag="PK264_08085"
     CDS             complement(10684..11262)
                     /locus_tag="PK264_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRD75882.1"
                     /translation="MASLAGQLLIAMPGMTDKRFARSVIYMCAHSNDGAMGIILNQHA
                     RNLTLLDLLQQLSVVPERSGAELPGALKDTSVHVGGPVSSGRGFVLHSGDYFVEDESL
                     RIDDQVSLTATVDILRSLVAGSGPRRFLLALGYSGWAGGQLEAEIQSNGWLHCDADAD
                     LVFDPNIDDKYSRAMSKIGVDVSFLSAAAGRA"
     gene            11470..>12333
                     /locus_tag="PK264_08090"
     CDS             11470..>12333
                     /locus_tag="PK264_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRD75883.1"
                     /translation="MIIRGQETVLVQGITGKQGTFWSERMLEYGTAVVGGVNPRRAGE
                     RHLGLPVWSSAVDAAREQRIDAAVMFVPPMGVKEAALDLIAAGIGKIVCLTEHVPVHD
                     TMYILAAARERGTRIVGPNTAGLVTTGQCFVGFMPAFNPRIFKPGNVGVISRSGSLGT
                     LLCLNLVQAGLGISAFVGIGGDPIIGTTTRDALVALDQDPATKAIALVGEIGGAMEEE
                     AAEYAATMTKPIAAFIAGAASPPGKKMGHAGAIVEGNRGSYAGKRAALEEAGVTVSAT
                     PGAVPAVLKARL"
     assembly_gap    12334..12351
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12352..13380
                     /locus_tag="PK264_08095"
     CDS             <12352..13380
                     /locus_tag="PK264_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HRD75884.1"
                     /translation="ENQREDDAMQTVYYAARGAAREVLRFEDGPPPEPGAGEVQVRVH
                     ASGINPSDVKTRAGANGRMTAPRTVPHSDGAGVITAIGAGVPDRRIGERVWLFNVNRT
                     ADGLGQGERGTAAEAIVVDQRLAVPLPANASFEIGACLGVPAMTAHRAVMADGSVAGK
                     SVLVTGGAGAVGSMAIQIARWSGAANVIATVSTPLKAETALADGAHATVNYKSEPLAE
                     RILAVNGGKRVDRIVDVDFAAHAEVAVKVLAPGGVIATYASMSNRTPALPFYPLMFND
                     ATIRLVAVYVMGWPAKEAAASDINQMLEAGKLRPRIAARYPLRSIVEAHETAERGDLV
                     GKVVVAID"
     gene            13468..16035
                     /locus_tag="PK264_08100"
     CDS             13468..16035
                     /locus_tag="PK264_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD75885.1"
                     /translation="MFFKTKKEPVPLAPEKPATSIAAPAGSRVTAASAIIARPDPLSV
                     GELRRIVDPATLGFKTTDELEPISGLIGQDRALKAIQFGANMKSHDFNMFVLGPPASG
                     KSTAVKAYLERKAREAPPTPDLVYVDNFEAPNKPKALTLPHGRARAFAKGMVAAIDEL
                     RNTLPAIFESDDYQVRRRAIDEEFRSGQEEAFEALSKRAQAQNIAVLRTPTGFAMAPV
                     HEGKVVKPEVFNSLPETMRKDVEAKIEVLQKELELILERVPKSDKQRRQRLAALNDEV
                     AKIVVREALDDVAAAFADVPGVTDYLNGAGADLVRNAGLFLPQGEEDGAAIKPSADTE
                     RDARFRRYMVNVFVENGSATGRSGAPILEEPNPTYANLIGRIEHIAQMGTLVTDFLLM
                     RPGAFHKANGGYLLLDARKLLLSPFAWEALKRTIKQREIRIEQPAEASLMMSTQSLDP
                     EPVPLDVKVILIGSREIYYLLAANDPDFQGLFKVQADFDDSIARSSENDRAYARLIAS
                     IVKEHKLRPVEAPAVARIIEEGARLADDREKLSIEIGRIADIVREADYWAGEAKRATV
                     TRQDVTRAIEEGVQRADRMRDRAQETIKRRIVLLDVTGAKVGQINGLSVLQLGNFSFG
                     RPTRISARVRLGNGRVTDIEREVDLGGPLHSKGVLILSGFLAGRFGQDMPIAMAASLV
                     FEQSYGGVDGDSASSTELYALLSALADVPIRQGIAVTGSVNQWGEVQAIGGVNEKIEG
                     FFDVCSAREEFRTFHRGLDGEQGVMIPRANVQHLMLREDIVAAVAEKKFSIWAVESID
                     RGIETLTGVKAGERGSDGRYPAGTINRLVEDRLRTFADKARAFAQKPGGDTDKAATS"
     assembly_gap    16055..16094
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16095..16920
                     /locus_tag="PK264_08105"
     CDS             <16095..16920
                     /locus_tag="PK264_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75886.1"
                     /translation="CETPSEVALDAAMRVARAFESEVESLFIENAQLYQLASYSFARE
                     ISLSGRQTRSLSEASISQQLKLAAHAVQRRIEALARIAEVPFSHRIVRDEPVPALARA
                     CSERGPWNVVALAEPFSPANAQELRHLFEEVTGTTGLVIVGPSARRTTGPVVAVVEDL
                     DHVEPMLRAAERLATITTAVGRAMAEPVTLVVVGEDRSARDWLEGQVRLMIARRDDVR
                     LSLPEETRGVADVIAEEIRRLGAGFVVSQFGGHVVPAGGDLRHLAIALECPLFLMR"
     gene            17027..>17554
                     /locus_tag="PK264_08110"
     CDS             17027..>17554
                     /locus_tag="PK264_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRD75887.1"
                     /translation="MTTHNLLLIPGDGIGPEVMAEVERVLALLNKSGKSTFKTDTALA
                     GGCSIDKHGVPLTDEALAKCKAADAIIFGSVGGPKWDKVKYENRPEAGLLRIRKELDL
                     FANLRPAICYPALAEASSLKRELVEGLDILIIRELTGGEDIDIVFEHPGRETFGASVF
                     VTRKGGTITTCVSTSG"
BASE COUNT         3010 a         5540 c         5768 g         2858 t
ORIGIN      
        1 cgagattgcc gagcacctcg gcgtccgact tggagacggt tttcggcggg atgcgcgcca
       61 tggtggcgac ggtcgcctcg acctccttca cgccgatctt cttcttacgc ttgccttcgg
      121 gcagcagcat ctgcgaggcg cccgtctcgt cgatcacgtc gatcgccttg tcggggagct
      181 tgcggtcgtg gatgtacttc gccgagagct cgacggcggc cttcacggcg tcgttcgagt
      241 agcggagctt gtggtactcc tcgaagtagg gcttcaagcc cttcaggatc tcgatcgtgt
      301 cctcgatcga cggctccttc acgtcgatct tctggaagcg gcggacgagc gcccggtcct
      361 tctcgaagtg ctggcgatac tccttgtagg tggtcgagcc gatgcagcgc accgcgcccg
      421 actggagcgc cggtttcaag aggttcgagg cgtccatgga gccgcccgag gtcgcgccgg
      481 cgccgatgac ggtgtggatc tcatcgatga acaggatcgc gcccggatgc tgctcgacct
      541 ccttgatgac ggccttcagc cgctcctcga agtcgccgcg atagcgcgta ccggcgagca
      601 gcgtgcccat gtcgagcgca aagatcgtgg cgccgcgcag cacctcgggc acctcgccct
      661 tgatgatctt ccgcgccagg ccctccgcta ttgcagtctt gccgacgccc gggtcaccga
      721 cgaacaatgg attgttcttc tggcggcggc agagcacctg gatggtgcgc atcacctcgg
      781 cctcgcgccc gatcagcggg tcgatcttgc cctcggcggc cttcttgttg aggtcgacgc
      841 agtacgtcga gagcgcatcg gcgctgttct tcttctcgcc cacaccatcg ctcccttggc
      901 tttcctcgtc gacgccgcga acaggccggg gctccgagag accggcacgc ttcgcgatgc
      961 cgtgactgat gtactggatg gcgtcgaagc gggtcatgtc ctgctcttgc aggaagaacg
     1021 ccgcgtggct ctcgcgctcg gcgaagatgg cgacgagcat gttggcgcca gtcacctcgt
     1081 cgcggcccga ggcctggacg tggacgacgg cgcggtggat gacgcgcttg aaagagttgg
     1141 tcggcgtcgc ctcggcggag cccttgacga cgagggacga ctgctcctga tcgaggtact
     1201 cggtgatctg cttgcgcagc acctcgaggt cgacattgca cgcgcgcatc acggcggcgg
     1261 catcgcgatc atcgatcagg gccagcaata ggtgctcgag cgtcgcgaac tcgtgatgcc
     1321 gctggttggc atactcgagt gcgcggtgga gggcctgctc gaggctctgc gagaaggttg
     1381 ccacgttcga ctattccttt tccatcgtgc aacgcagggg gtgctggtgc tgccgggcga
     1441 agtccataac ctgggttact ttcgtctccg cgacctcgaa cgtatagacg ccacagatac
     1501 cgacgccctt gtggtgcaca tgcagcatga ttcgcgtcgc atcctcgcgc cccttgctga
     1561 agaagcgctc gaggacgtgg atcacgaact ccatcggcgt gtagtcgtca ttgaggagca
     1621 gcaccttgta gaggctcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccgc gccgttcccg ccgctgccgt
     1741 tcccgcctgc catggtcgtc tcagtccgca ttgcggtttc ccgccccaat tgccaaccag
     1801 aacctcggtc gacccgatat ggacctatcc gagaagccgc ctcgtcctcg gattggaccc
     1861 gagaactgtg tcgttcatgc gtccagccta accctgccgc cgcttgttcg ccagtgcact
     1921 ctcgcgttaa tcggagatca gcgttgctcg agaggagtcc gtgcggctcg gcgacgggtg
     1981 aaagccgcca gactgccgaa aatcgtgtcg cccggttgtc tcgtcaagag ggcgcgcgac
     2041 gggctcgcat caggcgctcg aaaaggacgc cggccagcac gagagcaatg ccgaccccct
     2101 ggatgagctg cagcggctcg ccaagcacga gaacacccgc aagtaccacg gtgacgagct
     2161 ccaagctgac gatcagcgca tagcccgatg aggagagccg cggcaaggcg taggagaaag
     2221 ccaggatctg cagggcgccc ccgagcgtgc cgatgaagag caccgagagc cagagcgcgc
     2281 ctgtcggcgg cagtttcagt ccgcgagaaa gggcaatcga gccgaaggcg acggcgagcg
     2341 atccatagat gaacattgcg ctcgcaaatg gcgcgacgcg gcgggtcgca ggataggcga
     2401 cggcgatgta gagcgcatag ccgagagggg aggcgcaagc aaaggcgagg cccacgggat
     2461 ccagattggc gtccttaagc cccggcaggc tcgtcatcgc aacgccgacg atgacgacgg
     2521 cggcggcgag caaggtggtg ccgtagaccg gcagtttgaa gaagaagcgg tcgatcagga
     2581 tggtgacgag cgggtaggca tagaagaacg ttacgaccac gctcgtcggc agcgtctcga
     2641 ccgccttgaa atagaagaag gtctgcgccg agccgaggag cagcccgttg agaaaaaggt
     2701 gcgcgccgcc gcgacgccac tcctcggcga tgctcggcac ctgtgagagg gcgatcggaa
     2761 cgaggacgca gagtgcgacg aggtagcggt agaacaggat cgtcaccgtc tcgaggccgg
     2821 cattgtggag gcctgagaag aacagtggcg tgaagccgac gcagatactg ccgatcgcgg
     2881 cagcgagcgc ggcctttagc tcggagccgt gctcggcggc catgctcagc ccttcttttt
     2941 cttcgacgtg aagaaacggg cgatcgcagc ctgcggctcc ccggtcctat agctattgga
     3001 gagcgcctcg atgccggcga gcacgccttc ctcgacggac gagcgctgcc aatggtcgat
     3061 cagcgccttc tgcgcgcgga ttgccttggg cgtcgccttc aagatgccgc cgatccagtg
     3121 atcgacggct gcatcgagtc cggacgccgg cacgactttc tcgacgaggc ccatggcgag
     3181 tgccgcctcg gcatcgaaag tctcgccggt catgaggatt tcacgcgtct taccccagcc
     3241 gatgaggccg ggtagcagcg ccgcctcgat cacggagggg agccccatgt gcacctcggg
     3301 catgccgagt accgcgccgg cggccgcaac ccggaaatcg cacgacgcgg cgatctcgag
     3361 gcccgcgccg agcgtgaatc cgttgatgcg gccgatcacc ggtaccggac aatcgcggat
     3421 cgccttgcag gcctgatgaa gccgggtgat gtagaggcgg cccgagtcgt ggtcggcggc
     3481 gccgagttcc ttcaggtccg cgccaccgat gaatgcggta tcgcccgcgc cagtgaggac
     3541 gacgcaggcc agcgtctcgt cccgcgaaag ggcatggaat gtatcggcga ggagcaggtt
     3601 ctgcgcgctt gtcaggcaat tcaggcggcg ctggttgtca acggtgacgc gggcgacgcg
     3661 ccggctgccg tgatggtcct tgaacttggt cgcaatggtc ggcggctggt ccgacatggc
     3721 gtgctccgtg tctgttagat tagtgtcgcc ggacaggcta cagagctgcg cgagccgtca
     3781 ccagaggctt ctggcagagg cggcccgctt ggtgacggcg gatcgtgatt tgcgttctgg
     3841 tattcggctt cttgccagta cggaggcgtc aatgtctgca gcttccagca tctcgggtaa
     3901 gtggccagca ctgctgcgtg tgttcatgct cctcattttc cttggggcgc cgctcgtcct
     3961 cgtccgcgac gcgcaggacc tgtggcacga cttcgcaagt cgccaacaca cgttcgtgcc
     4021 ggcacccaac gtccggatcg tcgaagcgaa atgcagcatt ggcggtcggc aatgtacgat
     4081 caaagtcgac ggaggggtgt ctgtgagcgg tcgggccgag ttcaagtatg cgttcttcga
     4141 atccatgact ggcaagaccg tcgttccagt gcgctcgtcg gccgacggct cgatcacgat
     4201 caaggagggg cgggagcggc tcgggcaacg gctggccatg ctcattttcg ctgcactgct
     4261 gatcatcgcc ttgaacctcg ggctcattgc atcgtttgtc acggctgacc gccttcgtcc
     4321 gtacctcgac gctgcggcgc gcacatccct gctcgcaagc ctcgcagcct tcttcggcgg
     4381 cctaatcctc tacgccgccg gcgtgatagc ctgataggac ggtgctggat ggcgagtgac
     4441 tgacacgacg agtacgaccg ggaaccgcct atgcccctcc agaaccgtgt gctgccaaca
     4501 ggggagatcg tcgcctcgcc ggcgcgcgga ctcatgatgg gcaatcgcgg cggccggttt
     4561 catcgggcgg acaggacgct cgggcctcga cgctgggctt cgcgggcctg gatcgcctgc
     4621 cggctcaact tcaagggccg ccacagaccg gcgatgatga tggcgaactc gtacaccgag
     4681 ctcttttttc tcgacgaggc gaccgcgctc gctgccggcc accggccgtg cttcgaatgt
     4741 cgccgggcgg acgccgattg ctttgcgcgg ctgtgggccg ggctccgggg aggcaaagag
     4801 cgcgcgagcg ccgacgagat ggatcgtgcg ctgcatgccg agcggctcgc gggcccgagc
     4861 ggcccggacc gctggcgcgc gctccgcgat gtgccagacg ggacgatcgt cgaacaggac
     4921 gggccctgcc tcgtgctcgc cggcatgctc ctcccctggg cgatcacggg ctatcggccg
     4981 ccgctctccg ggcggccagc ggcggtgcgc ctcatcacac cgcccgccat tgttgccgta
     5041 atttcggccg gctatccccc gatggtgcat ccttccgccc acccggccgc cgcatcgatg
     5101 gcggtccgtt ccactctcga ttgagcagct catgacccgt tcgacactgc ggcgctcgat
     5161 gcgccgggtc tattggttcc tcggcctcat catcctggcg gccgtggtcg ccaagttcgc
     5221 cgagcacgtg ccgggcctca aggacacgct gatcctcggc gtgttcaagg acatctacga
     5281 gctcattcgc gacatggcgc tgctgatcgc gaccggcggt gtcgctgcga tcacgaatac
     5341 ctatcagcgc cgctcgcagt tcatcgaggc gctccgccag gaatggcgcg agatcgtgca
     5401 ggcgaagtcg gcagtgttca cctatacgca gcttgagaac ccgacgaccg agcaatacct
     5461 cgcgaccttc tgcaagctct ccgagacgat cgacaacatg cgcaccgtct atgcgaacgt
     5521 cggcgagacg ggagacctcg tcgggctcta cccgtacgcg ccgctccacg acatgcgccg
     5581 cgctctgcag acgctcgacc ctcgtctcgg cggcgacctg agcccggcgg cccgcaagaa
     5641 ggttcgcgat gcgacattgc aggtcttcta tgcggtgcgc gatcgcttcc tcgaggagct
     5701 cgacctcgag gagccggatg ctccggtgct gatctcgggg gcgcggcggc tcaagtcgtc
     5761 gggggcggct ggctatgccg gccgcatgca gggccgcgag gcgaaaggtg tcgcgaaagc
     5821 acccggcggc agcgccgaga tcgaccagtt cctggccgaa ctcaaggcaa aggaggatgc
     5881 aacctccaag atctggcgtc cggccgcggg gaacggcgcg gtaagcgcgc ccgggcgagc
     5941 ggcgccgccc tccaactcac gcccggcgag ctgaggtgta gatcggctcg gcgcgcgcag
     6001 cagtcgcgcg cttctcagcc tgctgccctt gctcgagcac gcgatgggcc tcgttgatgc
     6061 gtcgcacctg cgcggcgaga tagtcgcgga cctcggccgg cagatcggca cttgcatagc
     6121 gatcgggatg gtaggtcttc gccttggcgt ggaaagcctg ccgcacgatg tcgaaactgg
     6181 aaccacgcgg cacgccgagt acgcgccagg ggtcgaaacc gtcaaggtcg atgagccgcg
     6241 attcgagcga agccgccttg gtcccgccga ccaggcgcac ggctttcacc gctgtcttgg
     6301 cgaggaagcg gcggtcgccg tcgtagggct caaaatcgag gaaggcgacc tggctgttca
     6361 gcacctcgaa gagcgagcgc gacattggta tcgcgagctt tcccgagagc acctcgccgc
     6421 catccatcgt gatctcgacg ccgattgccg cctgctccag ggaattgtcg accttgttgc
     6481 gctcgaacat gcctgtctcc tgatgggccg attcactggc ccgcttcgat gcaggacggc
     6541 gaatgctggc gtctgggcag gacagctcgc aagacttgtg caagttttca gcggcttcaa
     6601 cgaagggcgc acgctgttct tccgggcatg gcggtggata cgcccgtgat tgtggcggcg
     6661 attggcccgc atgcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn cgagcctctc gccgagttca ttcagcgccg gctatgccga
     6961 gcaaccgaca gcctcggtac cggcagcgcg caagagcggc cgatccgccg tcggcgcaac
     7021 atccgacact gtgccgggat tgccggcccc gcccacgagc acgcctcccg tcaccgccaa
     7081 gacgcgcacg gccgatctcg cccccccggg cagcttcgat gtcgccccgc gcggcgcgct
     7141 ttcggggcgc gactactcgc tgacggccct cgacgtcgag cgcgcccgcg acctcatcaa
     7201 tgcctatcgc cgccagaacg ggctgaagcc gctgacgctc cacccgaagc tcaccgaggc
     7261 ggccaagctg cacgcccgtg atctcgccaa gtgggatcgc atttcgcact tcggatccga
     7321 cggctccaat ccctgggatc gcgtgaagcg gtcggggtac aacgcgcgcc tcgcggccga
     7381 gaacgtcggc acggggcagg tgacgttcga ggaagtcctg aagggctgga aggacagccc
     7441 cggccacaac aagaacctgt tgctcgccga cgcggaccat atcgggctcg ccctggtgca
     7501 ggacccgaag accgagttca agacgttctg gacgctggtg ctcggggcgc cgctctgatt
     7561 gctgaagtac ctgcgcgcag gcccgcgcaa cgcgcgtgtc acgggcgggc gcggagattt
     7621 tcccagatgg cgatcctgag gccgaggcgg cgacgtgccg aaagccactg cgcaaacgcc
     7681 gccgcgacga gcgcagtcga ggtcgcgatc gctcctccga tgagaccgag gctcggcact
     7741 agcagcagcc cgagcgcaat atcgaccacc gcagcaagca ccaggatcaa ggcgcaccgc
     7801 cgctgctcgc cgagcatgtt gagaaggaac tcggcaggcc cgacgatgga tcgcgccagg
     7861 aatcccaggg ccagaattgc catcacggca tagcctgacg tgaactgcgg gccgaacagc
     7921 cagagcagcg gcttgccgag tgcgagcaac aggccagcgc cgagaagcga aggccaaaac
     7981 gtccagttga cggcatcgcg gacaaggccc tcgagagcct ggcgatcacc gcggacatcg
     8041 agggccgcgt agcggttcgc aaccgcgctg ccgacggcgt agtgcacgaa caggatgagg
     8101 ctcatcgtct tggcggcggc gaaatagatc gcgacgtcgc tcggcgagag gtagcgcgag
     8161 acgatcaaca ggtcggcgtt ctgcaggacg agctcgcacg tggcgatcac gagcagggga
     8221 agtgcgatcc ggaaccagcc ggcgatatcg tagcgccgca cgtcggcgcg cggctcgtcg
     8281 gcgagcttgc gctcgatcag cgcgagctgg agaacggccg cgagccacgt tgcaacgacg
     8341 gccgcgcctg ccgcggtctt agcatccatg gcgaggccag agagccgggc aagccccatg
     8401 caggcgagca ccaggagggg gcggatgacg tagtgcggca ggagcccggc ggcgagccag
     8461 ccgctgccgc gaccaatgcc gtcgtggacc tccgacagcg tgaatagcgg caggcagaag
     8521 aggacgatga atgcggacag cacatagggg cctgataggc gctcgccggc gagccagatc
     8581 gccaggaggc cgaggccggc gatcagcgtc gccgcggcaa gaggaatgag ccggcccgcg
     8641 cgaaggagac catcgaagag gtgccgctcg ccgcgctccc gataggtcgg aagcaggcgg
     8701 atcatggtca tgccgaagcc gagcgtcgag acgccgccga gcatcagcac ggaggtccac
     8761 acgaagacgt agacgccgta ctcaaagctt cccatccacc gcgccaggat gacctgcgag
     8821 aggtagagga gggcggcact gaccgcgcgc acgagaaagg ccaggatggc atcgcgtccg
     8881 gcgcgattgc gatcgctcga gccgcgcagc agcgcgccga gcagcgtgcg ggcgccgatc
     8941 ggatcgtgaa tcggctcagc cagagccggc gctgcagtcg ggtcggcctt cattgcatcc
     9001 tctgcgccgc cggcgccgca actcaggctg caggcggaac gatcatggta gcgaaaatgg
     9061 tttacgaact cgtcatgcgc gccctttcgc gccggcggat gatctcggca acgccagccc
     9121 aggcccagca acggccggca tcgccgtgat ccccgtgcgc tgcagcaaag aattccgcga
     9181 cctcggccgc atagacgcca tgtcggccct cgaggacgct cgccatcatg tcgacctcgc
     9241 gcggcgtcgg cgtcgtatcc atctcgggat gccacggggc ggcggtagtg tggcgcatca
     9301 tgctgtcgcg ttccttgcca tgtgtctcgt cgcagtcaat tcgacgtgag caagaaggac
     9361 gccagctgcg atcagcaact cgggtgatgc ggcgtcaaac ggccgccaag gtaaaccgcg
     9421 cgactcggga tggacggaat atgggcaagc gggcgcggcc tatgaggccg aaacgaagaa
     9481 ggggcccatt gggccccttg caatcctcgg ctgcacgacg tcggccaggt aagccttttg
     9541 ccgcatcggc gtcagtcttc agttgagcag ctccttgagg gccttggcgg cctggaactt
     9601 cggcacgttg ctcgccggga tcttgatcgg ctctttggtg agcggattct tgcccgtgcg
     9661 cgctgcacgc ttgctcacct tgaaggtgcc gaagcccggg atgcggactt cctcgccctt
     9721 cttcatcacc tcgccgatgt gcttgatgac ggcatcgacg actgttgcgg ccttgtcttt
     9781 ggtgagctcg gcttccttgg ccacggcgtc gatgaggtct ttcttgctca tgatcatttc
     9841 cttctgatgc ccggtccttg tcttggccgg tcactccggc accccgtctc gctcgagggg
     9901 acgcccgtgt tgccgaagga caagagccgc gagcacgatc gtcggagccg ggcagtgaag
     9961 tgccaggtcc tcgagtttga gctcttcgca tgcacttgcg cctcgacggc ggcagggcac
    10021 acaaaccact ttgctgcgag gcgtcaagcc caaatgcatg cgggaaagcc catggatagg
    10081 gcaattttca accgatttgc aggtgaactg aggtgtccgg gcgggcgtcg gcaggtgtca
    10141 cgccgggaga ggtggctccg cttgagcaac agcaagtcgc caaacacaag ggcgtgtcgc
    10201 caaacaatta gggcgcggcc actgtcgacc gcgccccgtc cttgtcaggg gtcaggcttc
    10261 tcagccgatc atcccgagtg tgattgccgc gacgaaaagg aatgcgcgag aacattcaat
    10321 ctcagcttga gctgcatcca aagtctcctt cttgtccgtg ccccttcgtg cccttgctcg
    10381 ttggagtcct gtgggcatcg gtcaggcaaa gccgttgggg gatgacccac cggcggcatg
    10441 gggcaagaat agcggctgag gccaattaaa gccaaggaat ttcgttgttg cgctgcagca
    10501 tcgcgtcagc tcagtggagt gtattccagc attgcgtcga ggcaacgcgg tgttgctgca
    10561 aaacactaaa acacaatact gccctcgtat cgtgatatat ttcacatgaa caagcgttaa
    10621 ctaaatagac ctggtgaaca tgattggatg tcatcgaata tacttagagt attcgactgt
    10681 gtctcaagcc cgccctgctg ccgccgacaa aaagctcacg tcgacgccga tcttcgacat
    10741 ggctcgactg tacttgtcgt cgatgttcgg atcgaagacc agatcggcat cggcgtcaca
    10801 gtgcagccat ccgttcgact ggatttcggc ctcaagctgg cctccggccc aacccgaata
    10861 gccgagcgcc agcaggaagc gccttggccc gctgccggcg accagcgatc gcaggatgtc
    10921 gaccgttgcg gtcaagctca cctgatcatc gatacggaga gattcgtcct cgacaaaata
    10981 gtcgcccgag tgcagcacga agcctctgcc cgacgagacg ggaccgccga catgcaccga
    11041 ggtgtccttc agcgccccgg gaagctcggc tcctgagcgc tcgggaacga cgctcagctg
    11101 ctgcaggagg tcgagcagcg taaggttccg ggcgtgctga ttgaggatga tgcccatcgc
    11161 tccgtcgttg gagtgggcgc acatatagat gaccgagcgc gcgaagcgct tgtccgtcat
    11221 gccgggcatg gcgatcagca gctggccggc aagtgacgcc atcgcgtccc gacgtgattt
    11281 tcgcggtgtc ctcatgcaat cgagagtagc cggagcaggt ccgacttgga agagccgtcg
    11341 gggcggcgca cggcccgcgt ggcggatggc aggacgcggc gcacggagcc gcgcgacggt
    11401 tgccaaaaga gccctcgttg gaccacagta gcccgattgg gcggactgcg tgcgccgggc
    11461 ggagcttcca tgatcattcg cggacaggaa acggtgctcg tgcagggcat tacgggcaag
    11521 cagggcacat tctggtcgga gcgcatgctc gagtacggta ctgccgtcgt ggggggcgtc
    11581 aacccgcggc gcgccggtga gcgccatctc gggctgcccg tctggtcttc tgccgtcgac
    11641 gcggcccgcg agcagcgcat cgatgctgcc gtgatgttcg tcccgcccat gggcgtgaag
    11701 gaggcggcgc tcgacctcat cgccgccgga ataggcaaga tcgtttgcct caccgagcac
    11761 gtgcccgttc acgacaccat gtacatcctc gccgccgccc gtgagcgtgg cacgcgcatc
    11821 gtcggtccca acacggccgg cctggtgacg acgggacaat gcttcgtcgg cttcatgcca
    11881 gccttcaatc cgcgaatttt caagccgggc aatgtcggcg tgatctcgcg ctcggggagc
    11941 ctcggtacgc tcctctgcct caatctggtg caggccgggc tcggtatctc cgccttcgtc
    12001 ggcatcggcg gtgacccgat catcggcacg accacgcggg atgcactcgt cgcactcgat
    12061 caggacccgg cgaccaaggc catcgcgctc gtcggcgaga tcggcggggc gatggaggag
    12121 gaggcggccg agtatgcggc gaccatgacg aagccgattg cggctttcat cgctggcgcg
    12181 gcgtcgccac caggcaagaa gatggggcac gccggcgcga tcgtcgaagg taatcgaggg
    12241 agctatgcag gcaagcgggc ggcgctcgag gaggccggcg taacggtatc ggcaacaccg
    12301 ggtgccgtgc cggccgtgct caaggcgcgg ctcnnnnnnn nnnnnnnnnn ngagaatcaa
    12361 cgcgaggacg acgcgatgca aactgtctac tacgcggcgc ggggcgctgc gcgtgaggtg
    12421 ctccgcttcg aggatggtcc gccgccggag ccgggcgccg gcgaggtgca ggtgcgcgtg
    12481 cacgcgagcg gcatcaaccc gtcggacgtg aagacccgcg cgggtgccaa tggtcggatg
    12541 acggcgccgc gaacggtccc gcattccgat ggcgcgggcg tgatcaccgc gatcggcgcg
    12601 ggcgtgcccg atcgacgcat cggcgaacgg gtctggctct tcaatgtcaa ccggacagcc
    12661 gatggcctcg gccagggcga gcgcggcacg gcggcagagg cgatcgtggt cgatcagcgt
    12721 ctcgccgtgc cgctgccggc aaatgcctcg ttcgagatcg gtgcctgcct cggcgttccg
    12781 gccatgacgg cccaccgcgc cgtgatggcg gatggctcgg tcgccggcaa gtctgtgctg
    12841 gtgacgggcg gcgccggtgc cgtcggttcg atggcgatcc agatcgcgcg ctggtcgggt
    12901 gccgccaacg tgatcgcgac ggtcagcacg ccgctcaagg cagagacggc gctggcggat
    12961 ggcgcgcatg cgacggtgaa ctacaagtcg gagccgctgg ccgagcgaat tctcgcggtc
    13021 aacgggggaa agcgcgtcga tcgcatcgtc gacgtcgatt tcgcggcgca cgctgaagtg
    13081 gccgtcaagg tgctggctcc gggcggcgtg attgcgacct atgcctcgat gagcaatcgc
    13141 acgcccgcct tgccgttcta tccgctgatg ttcaacgacg cgacgatcag gctcgttgcc
    13201 gtctacgtca tgggctggcc cgcgaaggag gcagcggcca gcgacatcaa tcagatgctc
    13261 gaggctggca agttgcgccc gcgtatcgcg gcgcgctatc cgctgaggag catcgtcgag
    13321 gcgcacgaga cggcagagcg gggcgatctc gtcggcaagg tcgtcgttgc gatcgactga
    13381 cgacccaccg gccgttgccg gtcaaggacg ccggccatcc gcgatgcgga tgcaatcgat
    13441 tttcctgaac agtggggtca ctccgagatg ttcttcaaga caaagaagga gccggtgccg
    13501 ctggcgcccg agaagccggc gacgtccatt gccgccccgg caggcagccg cgtgaccgca
    13561 gcatcggcaa tcattgcacg tcccgatccg ctgtcggtcg gcgaactccg gcgcatcgtc
    13621 gatccggcaa ccctcggctt caagacgacg gacgagctcg agccgatctc gggtctcatc
    13681 gggcaggacc gtgccctgaa ggcgatccag ttcggcgcca atatgaagtc gcacgacttc
    13741 aacatgttcg tgctcggccc gccggcctcc ggcaagagca cggcggtcaa ggcttatctc
    13801 gagcgcaagg cgcgcgaggc cccgccgaca cccgacctcg tctacgtcga caacttcgaa
    13861 gcaccgaaca agccgaaggc gctgacgctt cctcacggcc gcgcgcgcgc cttcgccaag
    13921 gggatggtcg cggccatcga cgagctgcgc aacacgctgc ccgcgatctt cgagagcgac
    13981 gactatcagg tgcgccggcg agcaatcgac gaggagttcc gctcgggcca ggaggaggcg
    14041 ttcgaggcgc tctccaagcg ggcgcaggca cagaacatcg ccgtcctgcg cacgccgacc
    14101 ggatttgcca tggcgcctgt gcacgaaggc aaggtcgtga agccggaggt gttcaactcg
    14161 ctgcccgaga cgatgcgcaa ggacgtcgag gcgaagatcg aggtcctcca gaaggagctc
    14221 gagctgatcc tcgagcgcgt gccgaagagc gacaagcagc gccgccagcg gcttgccgcc
    14281 ctcaatgacg aggttgccaa aatcgtcgtc cgcgaggcgc tcgacgatgt ggccgccgcc
    14341 ttcgccgacg tgccgggcgt gacggactac ctcaatggcg ccggcgccga tctcgtacgc
    14401 aacgcggggc tgttcctgcc gcaaggcgag gaggacggag ccgccatcaa gccttcggct
    14461 gataccgagc gcgatgcccg cttccgccgc tacatggtga acgtgttcgt cgagaatggc
    14521 agtgccaccg gccgcagcgg ggcgccgatc ctcgaggagc cgaacccgac ctacgcgaac
    14581 ctcatcggcc gcatcgagca catcgcccaa atgggaacgc tcgtcaccga tttcctgctg
    14641 atgcgccctg gcgccttcca caaggccaat ggcggctatc tgctgctcga cgcgaggaag
    14701 ctcctcctct ccccgttcgc ctgggaggcg ctgaagcgca ccatcaagca gcgcgagatc
    14761 cgcatcgagc agccggccga ggcctcgctg atgatgtcga cgcagtcgct cgatccggag
    14821 ccggtgccgc tcgatgtaaa ggtcattctg atcggcagcc gggagatcta ctacctgctt
    14881 gcggcgaacg atcccgactt ccagggtctc ttcaaggttc aggccgactt cgacgacagc
    14941 atcgcgcgct cctccgagaa cgaccgggcg tatgctcgtc tcatcgcttc gatcgtcaag
    15001 gagcacaagc tccggccggt cgaagcgccg gccgtcgcgc gcatcatcga ggagggggcg
    15061 cgcctcgccg acgaccgcga gaagctctcg atcgaaatcg gccgcatcgc cgacatcgta
    15121 cgcgaggcgg actactgggc gggcgaggcc aagcgcgcga cggtgacgcg ccaggacgtg
    15181 acacgggcga tcgaggaagg cgtgcagcgc gccgaccgca tgcgcgaccg ggcgcaggag
    15241 acgatcaagc ggaggatcgt gctgctcgac gtgacgggtg ccaaggtcgg ccagatcaac
    15301 ggcctctcag tgctccagct cggcaacttc tcgttcggtc ggccgacgcg catctcggcg
    15361 cgggtgaggc tcggcaatgg tcgcgtcacg gatatcgagc gcgaggtcga tctcggtggg
    15421 ccgctgcact cgaagggcgt gcttatcctg tcgggcttcc tcgccggccg cttcggccag
    15481 gatatgccga tcgccatggc cgcaagcctc gtcttcgagc agtcctatgg cggcgtcgac
    15541 ggcgacagcg catcctcgac ggagctttac gctctactct cagcgcttgc ggacgtgccg
    15601 atccggcagg gcatcgccgt cacaggttcg gtcaaccagt ggggcgaggt gcaggcgatc
    15661 ggcggcgtca acgagaagat cgagggcttc ttcgacgtgt gctcggcgcg cgaggagttc
    15721 cgcaccttcc accgcggcct cgacggcgag cagggggtga tgatcccgcg cgcgaacgtg
    15781 cagcacctga tgctgcgcga ggacattgtc gcggcggtcg cggaaaagaa attcagcatc
    15841 tgggcggtcg agtcgattga tcgcggcatc gagactttga ccggcgtcaa ggcgggcgag
    15901 cgcggctccg atggacgcta tcctgccgga accatcaacc ggctcgtcga ggaccggctc
    15961 cggacattcg ccgacaaggc gcgagcgttc gcgcagaagc cgggcggcga taccgacaag
    16021 gcggcgacgt catgatcacg cacattgcgg cagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnctgcga gacgccgagc gaggtggccc tcgatgcagc catgcgggtg
    16141 gcgcgtgcgt tcgaatccga ggtcgagagc ctgttcatcg agaatgcgca gctctaccag
    16201 ctcgcgagct actcattcgc ccgcgagatc tcgctctcgg gccgccagac gcggagcctc
    16261 tccgaggcct cgatctcgca gcagctgaaa ctcgccgcgc acgccgttca gcgccggatc
    16321 gaggcgcttg cccggatcgc cgaggtgccg ttcagtcatc gcatcgtcag ggacgagccc
    16381 gtcccggcgc tggcgagggc ctgttcggag cgtggcccct ggaacgtggt ggcgcttgcc
    16441 gagccgttct cgcctgcaaa tgcgcaggag ctgcgccatc tcttcgagga ggtgaccggg
    16501 acgaccgggc tcgtcatcgt cgggccgagc gcacggcgca cgactggtcc ggtagttgcc
    16561 gtcgtcgagg atctcgacca cgtcgagccc atgctgcgcg cggcggagcg gctcgccacg
    16621 atcacgacgg ccgttgggcg tgcaatggcg gagccggtga cgctggtcgt tgtcggcgag
    16681 gaccggtccg cgcgcgactg gctcgaaggc caggtccgat tgatgattgc ccggcgggac
    16741 gacgtccggc tctcgctgcc ggaggagacg cgcggtgtcg ccgacgtgat cgcggaggag
    16801 attcggcgcc tcggcgcggg cttcgtcgtc agccagttcg gcggacatgt ggtgccggcc
    16861 ggcggggacc tgcgccacct agccatcgct ctcgaatgcc cgctgttcct catgcggtga
    16921 cgcgcccggc cctggcgccg gttgcagagc tcggcgcctt gcgcctcgag gcaactgccg
    16981 ctagcacttc gcaatcagtt ctcgcgacag agcacggacg cgccccatga ccacccacaa
    17041 cctgcttctc atccccggcg acggcatcgg ccccgaggtc atggccgagg tcgagcgcgt
    17101 cctcgcgctc ctcaacaagt ccgggaagag cacgttcaag accgataccg cactcgccgg
    17161 cggctgctcg atcgacaagc acggcgtgcc gttgacggac gaggcactcg ccaagtgcaa
    17221 ggcggcggac gccatcatct tcggttcggt cggcgggccg aagtgggaca aggtgaagta
    17281 cgagaaccgg cccgaggcgg gcctcctgcg catccgcaag gaactcgacc tcttcgcgaa
    17341 cctgcgcccg gcgatctgct atccggcgct ggccgaggcc tcgagcctga agcgcgagct
    17401 cgtcgagggg ctcgacatcc tgatcatccg tgagctcacc ggcggcgagg acatcgacat
    17461 cgtgttcgag caccccggcc gcgagacctt cggcgcctcg gtgttcgtca cccgcaaggg
    17521 cggcaccatc acgacctgcg tgtcgacctc gggc
//
LOCUS       DAORNH010000058        17453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2461_length_17453_cov_2.743591, whole genome shotgun sequence.
ACCESSION   DAORNH010000058 DAORNH010000000
VERSION     DAORNH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17453
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_17453_cov_2.743591"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PK264_08115"
     CDS             complement(<1..822)
                     /locus_tag="PK264_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD75888.1"
                     /translation="MKLLVTGACGYKGSVLVPKLLRAGHEVVALDTQWFGNEHVPHPD
                     LRVVRGDVRKPEDIDLTGVDAIIHLASVANDPCGDLDPKLTWEISCLATMQLVDKAKR
                     QGIKRFIYASSGSVYGVKDEDQVTEDLELLPISEYNKTKMCAERIVLSYASDMVVQIV
                     RPATVCGFSPRMRLDVSVNMLTMQALRNGKITVFGGDQTRPNIHIDDITDLYIFLLDH
                     PEHTGVFNAGFENISIMDIAQRVTAHVPAEIVVTPSNDPRSYRVNSDRLLATGFKP"
     gene            complement(1027..1740)
                     /locus_tag="PK264_08120"
     CDS             complement(1027..1740)
                     /locus_tag="PK264_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD75889.1"
                     /translation="MRALLLAAGMGTRLRPLTLSVPKCLVAVHGRPLLDYWLDLLFGA
                     GIDRAAVNCHWLAERVVAHVGASPWRDRVDLVHETELLGTGGTILANRQWLAGGPFLV
                     AHADNLTDFNVAALIAAHSSRPIGCAITMLAFRTDDPRSCGILELDHRGVVQAFHEKV
                     ESPPGDLANAAVYIMEPEVIDFIAAKGRPVVDLSTEVLPHYIGRIAAVVTKGYHRDIG
                     TLESLARANREFTPPQLSR"
     assembly_gap    2005..2018
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2470..3945)
                     /locus_tag="PK264_08125"
     CDS             complement(2470..3945)
                     /locus_tag="PK264_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRD75890.1"
                     /translation="MTADSGLYESVISEVGRDKRICFVSGNFNVLHPGHMRLLKFAAE
                     LSDFLFVGVNMDTTPGVTVPGPERLANIRELSLVKYAVLLDEPAPSFIAKLKPAIVVK
                     GREFEGRENPEQKVVDSYGGRLVFGSGETQFVSYSLLDEEFADTNRSTIVKPSEYPQR
                     HGFTLDGLRTWLKKVEGIRVLVIGDLIIDDYITCDPLGMSQEDPTIVVTPIETKTFVG
                     GAGVVSAHARGLGASVQFFTVVGADDASAFAYRNLQAQGIGVHAFTDDTRPTTRKQRY
                     RAAGKTLLRVNHLRQHAVSQALWRNMLAQIEERLPHTDLILFSDFNYGCLPQPLVEAV
                     VERARARKIPMAADSQASSQISDISRFKGMSIITPTEREARLALRDTSSGLAVLANGL
                     VQQAEAEAVIITLGSEGLLVHGKDQGTYYTDKLPAMNKAPKDVAGAGDSLFTATSLAL
                     CAGAGIWESVYLGSIAAACQTGRVGNAPLKLADLLVELGNA"
     gene            complement(4040..4948)
                     /locus_tag="PK264_08130"
     CDS             complement(4040..4948)
                     /locus_tag="PK264_08130"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75891.1"
                     /translation="MTRVLVTGARGFVGRWIAEGLVKRGHDVHGATSADPDLTLAGVT
                     WHLADLMQRGVAAELMTTVRPELLVHAAWFTGHGAFWTSDLNYRWVAASLELIEAFAS
                     TGGKRFVGVGSCAEYDWRHGFCAEGVTPLVPGHPYGVCKVALMRMAEAYAANHGLSFA
                     WARIFYPFGQGEPPARLVSSVIRALQARREAPCTAGTQVRDFLHVADCGDAIAALAAS
                     PVGGAVNIGCGEPRALRDVVMRIADRLEARSLVKLGALPMRPDDPPVLIPDVRRLGVE
                     VGWSPARSIDAGLDEAIAWWSSRGGA"
     gene            complement(4945..5478)
                     /gene="rfbC"
                     /locus_tag="PK264_08135"
     CDS             complement(4945..5478)
                     /gene="rfbC"
                     /locus_tag="PK264_08135"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRD75892.1"
                     /translation="MKIAETPLAGAYVIELEPIADERGSFARSFCEREFAAAGLATRF
                     PQWSVSRNATRHTLRGMHYQEAPHAEIKLVRCTAGSVRDVIVDLRTESRTYCKWFAVD
                     LTAESGRALYIPEGFAHGFQTLTDNAEVTYHISAFYVPGAGRGVRWDDPAFAIAWPEA
                     PARIMSERDRTYPDFVP"
     gene            complement(5475..6713)
                     /locus_tag="PK264_08140"
     CDS             complement(5475..6713)
                     /locus_tag="PK264_08140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019566370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD75893.1"
                     /translation="MTTHVCRFCSGLLAQSFCDLGTSPASNSFIRPENALHPEPFYAL
                     NVYVCGKCLLVQLPEHKAADQIFTDDYAYFSSFSTSWLAHSKAYCEMAAKRFGLGPKS
                     QVIEIASNDGYLLQYFKAMGIPVLGVEPTANTAKAAVEKGVPTLVRFFGRELAGELAR
                     DGRKADLLLGNNVLAHVPDINDFVAGMKLALKPTGVVTMEFPHLAKLIAGIQFDTIYH
                     EHYSYLSLIAVERIFAHHGLTVFDVDEIATHGGSLRIYARHAEAKTPERSARVPALIE
                     RERAAGLGSVAGYEGFDARVQEVKRSLLEFLIKVKREGKSVVGYGAAAKGNTLLNYCG
                     IRQDFIDFVVDRSPAKQGRLLPGTRIPVKAPEAIDSARPDYLLILPWNLRDEIVSSMG
                     HIRGWGGKFVVPLPKVEVIA"
     gene            complement(6713..7774)
                     /gene="rfbG"
                     /locus_tag="PK264_08145"
     CDS             complement(6713..7774)
                     /gene="rfbG"
                     /locus_tag="PK264_08145"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRD75894.1"
                     /translation="MTPAFWKGKRVLLTGHTGFKGAWAALWLSSLGARVTGFSLAPET
                     EPSLFRVARVGETMASIIADLRDPAAVDAAVSSAEPEIVLHMAAQALVRRSYRDPVAT
                     YATNVMGTVHLLDAVRRLSPARAVVVVTSDKCYENREWVWPYRENEAMGGYDPYSNSK
                     GCAELVTAAYRKSFLAEKGVGVASARAGNVIGGGDWSEDRLIVDLVRGFASGNPVLIR
                     QPKSVRPWQHVLEALVGYFTLAEKLHGEPARYAEGWNFGPAPDDAIPVGEIADRMAAR
                     WGDGATWVRDGQQGPHEAHLLRLDASKARAALGWRPRLPLDQGLDWIIDWHRAERDGK
                     DMRAVTLDQIARFQAMGAP"
     gene            complement(7771..8544)
                     /gene="rfbF"
                     /locus_tag="PK264_08150"
     CDS             complement(7771..8544)
                     /gene="rfbF"
                     /locus_tag="PK264_08150"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HRD75895.1"
                     /translation="MKVVILAGGLGTRISEETQVRPKPMVEVGGRPILWHIMKIYSAF
                     GANDFVICLGYKGYVIKEYFANYFLHMSDVTFDMEKNSMTVHEAKAEPWRVTLVNTGD
                     DSMTGGRLRRVKSYVAGEREFCFTYGDGVADVDIAKLIAHHRASGKLATMTATQPPGR
                     FGAADIDSGVVRRFVEKPRGDGSWINGGFFVLSPKVIDYIVADSTTWEREPLERLAAE
                     GQLAAFQHSGFWHPLDTLRDKNYLEELWTTGKAPWRVWK"
     gene            8992..10080
                     /locus_tag="PK264_08155"
     CDS             8992..10080
                     /locus_tag="PK264_08155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRD75896.1"
                     /translation="MVRGEDGTVSRDLRSDRRTDTVSVILPNYNHARYLEQSISAVLR
                     QTRPAEQVVIIDDGSTDGSLQLIERLVAGNPTVEVIRNARPSGVVSAMNQGLALARGT
                     LVAMLAADDMVFPTFFERSTALLNQHPDAAFSCALTEIWDEDGEVTGSRPFLRPALRE
                     RYFSPDATRQLLRKGDNYFLAASSLFRYSKIAEMGGFKGSLASAADGFLQRRMAVRWG
                     FCFIPAPLAAWRLHGANYSQASVTNPESLNEMLAQITRQINAEPAGLFPDGYAELFER
                     RLRFGAGRLIASRLPADPVASDQIAKCIKAGSIERRWLATLARTGRLSPRLMIAWLAL
                     RNRPFSPWWTLAESTSLATGRRRGSSSQ"
     gene            10146..>10829
                     /locus_tag="PK264_08160"
     CDS             10146..>10829
                     /locus_tag="PK264_08160"
                     /inference="COORDINATES: protein motif:HMM:NF022436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HRD75897.1"
                     /translation="MDNSPNLQLPYILAAQAQKHVTHNEAVRALDALVHLSVLDRSLA
                     TPPATPADGDRYLVAAGATGAWSGASGRIAAYQDGAWAFLTPKEGFIAWIADENVLLA
                     FDGATWQSALGSGADITADTLGINATADTTNRLSLASPATLFNHEGAGHQLKINKATA
                     ADTASILVQTGFTGRAEIGTVGDDHLQVKVSADGMTWLTALLVDKTSGQVWLPYTATG
                     VTSLLLNGID"
     assembly_gap    10830..10839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10840..11619
                     /locus_tag="PK264_08165"
     CDS             <10840..11619
                     /locus_tag="PK264_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75898.1"
                     /translation="NGDFQINQRLFAGGALAAGAYGFDRWKGGASGASCSVSGMIVTL
                     TSGSLTQVIEVAQRGHATLAAQTVTVSVDALSGGNLTVTLGSQTGTITAGSGRRSTTL
                     TLEVGDTGNIALTLAPVSAAVTFSRVRAELGRHASAWLAPSRDIELSACQRYYWRVGG
                     VAFNPIGIGRFASTTQGEVYLQLPTEMRTIPTVSTNGVANFSFNGVVLTGLTLHAGFN
                     QRRDFVGLLSTVGSAAGTPGDMAVLVSQPAPAGIIEFSAEL"
     gene            complement(11639..12910)
                     /locus_tag="PK264_08170"
     CDS             complement(11639..12910)
                     /locus_tag="PK264_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75899.1"
                     /translation="MLLARSSEGLWSLFASAVALVAPFLSFIVLEYFALPINAFTFRQ
                     WEALIPENGLNARYAFFPNATLQQWEYGDVHRYATITLPRTTRWFTDQLGYRNRAAYS
                     AVKTYCFVTLGDSNVIGASLDQSETLSEALERRARCPAYNGSSAHRLARFLNQPEFKA
                     NQPRFVVLQAIAGEFYSDQAYSRFDVSGQLIGVDPEPGIGYRAISAAIAVIAPDSPRA
                     SPWMLEEIVRRNAGWQFIRARLGMTDLPPPPATHRHNEARNIHDGRAAAASRAPRAVG
                     QVRYPTASKEVAERNCPEVIKALTDANARAANCRLIAVVRAASVALKSFGSDLIVYLQ
                     PSTDELLATGIAVLEAEGHKIIHFPKTSKLPYGIDFDWYWASDDSHWHPRGVELAAEL
                     ILAESEGRSAEALLPAVHERIEREMRALRRR"
     gene            complement(12913..13491)
                     /locus_tag="PK264_08175"
     CDS             complement(12913..13491)
                     /locus_tag="PK264_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75900.1"
                     /translation="MLRTITTTAIAALTLGLYGWSSMRGPDCLYRVCIGAPLPPLTEL
                     GPAAGKAVTRPDGVLEIDIRSLAPFADIVQFRPPFPTPAREGRATFALLRISRDTGGR
                     VDQVSLIVEFAEGSYLWAPQQGALLDKELARLVGAMGKRQEANWMRRYVWERLNRWRG
                     TRVTGEVWPGTGGIAALKLDLGTTAKVRGVEK"
     gene            complement(13514..14992)
                     /locus_tag="PK264_08180"
     CDS             complement(13514..14992)
                     /locus_tag="PK264_08180"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRD75901.1"
                     /translation="MVFTSLEFVIFFTVFYLLFIVTPQSYRLHLLLGASLFFFGYAVP
                     KHLWLLLLTIAISYVCGIGIAADRGRRKIYLWGGIVSLLGLLAFFKYTDFAISLANSA
                     LSVVKPGATITPLGILLPIGISFFVFQAISYLVDVYRGDKAAEHNIIVLALFKAFFPQ
                     LVAGPIERSTNLLPQIRKVFVEPRSEPLVSREAMQVGSQLILLGFFKKVCIADNLALF
                     ADHVFAEPGKHGPLAATLGVYCFAFQIYCDFAGYSDIARGVSRIMGIDLMENFRRPYW
                     STSIREFWTRWHISLSSWFRDYLYKPLGGSQVGAHRWALNIMAVFLLSGLWHGAAVNF
                     IIWGGVHGVIYLFENGLAKLRPRPLAATAGPGLRGAIAGQLGWLLAMFTTFHVVCFAW
                     IFFRARTFGEAIGVLQSIASIGQVPLWKGFELFERMSFAWCVLFVAILLVIDAWAEWG
                     GKLLASLAQNDTARWTMYYAAAAAIIVFGNWQSSQQFIYFQF"
     gene            15486..16295
                     /locus_tag="PK264_08185"
     CDS             15486..16295
                     /locus_tag="PK264_08185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRD75902.1"
                     /translation="MASNDNSSPATPPKPPGYTFLASVIHPYDPIVKANINHLTRGYF
                     DEHIVKGFIERIEGHTLVTYDGLMELANEVRFCELNNIEGDFVELGAYKGGCIAMMAL
                     CNMHYGKKRRMLHAFDSFEGLPQPVAKDLDAHFDGIFKLRDDQKQGKLQSINAVDAGE
                     EYLNQVVFEKVKYPREFVKIHKGWFQNTVPVAKHEIDKIAILRLDGDLYDSYMVPLEH
                     LFDKVVPGGFIIIDDWIFTGCREAIKDYFEPRGGIPYLHHVDITVRVIRKM"
     gene            complement(16349..16840)
                     /locus_tag="PK264_08190"
     CDS             complement(16349..16840)
                     /locus_tag="PK264_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD75903.1"
                     /translation="MVAEAHSGRCTCGAVTYRIMAAPIFVNCCHCTWCQRETGSAFAI
                     NAMIEADRVEVMSGAPEQVLTPSNSGKGQRIWRCPSCRVALWSNYPGAGPSINFVRVG
                     TLEAPHPFVPGAHIFTSTKVAWVVVPEGVPSFAEFYDAATMWSADGLARFKAARSKHV
                     AAG"
     gene            17016..17264
                     /locus_tag="PK264_08195"
     CDS             17016..17264
                     /locus_tag="PK264_08195"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRD75904.1"
                     /translation="MSTIVERLYHAGSTTETQRSRRSVSAATRLAGAWQRYIRQRRTE
                     RALEGLSEATLKDIGIHRSEIRRVSRDAIEARDWRHTH"
     gene            17299..>17453
                     /locus_tag="PK264_08200"
     CDS             17299..>17453
                     /locus_tag="PK264_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75905.1"
                     /translation="MINHISIGVTDIAAARRFYDAALGPLGYRCLSSGETSLGYGRTA
                     PALWLIAT"
BASE COUNT         3196 a         5857 c         5476 g         2900 t
ORIGIN      
        1 cggcttgaag cctgttgcga gcaggcgatc cgagttgacg cggtacgagc gcggatcgtt
       61 cgacggcgtc accacgatct cggccggcac atgcgcggtc acacgctgcg caatgtccat
      121 gatcgagatg ttctcgaaac cggcattgaa caccccggtg tgctcggggt gatcgagcag
      181 gaagatgtag agatcggtga tgtcgtcgat gtggatgttg ggacgcgtct gatcaccgcc
      241 gaacaccgtg atcttgccat tccgcaacgc ctgcatcgtc agcatgttga cggaaacgtc
      301 gaggcgcatg cgcggagaga agccgcacac tgtcgccggc cggacgatct gcaccaccat
      361 gtcgctggcg taggagagca cgatgcgctc ggcgcacatc ttcgtcttgt tgtattccga
      421 gatcggcagc agctcgaggt cttccgtgac ctgatcctcg tccttcacgc catagacgct
      481 gcccgaggac gcatagatga agcgcttgat gccctgccgc ttcgccttgt cgacgagctg
      541 catggtcgcg aggcagctga tctcccaggt gagttttggg tcgaggtcgc cgcaaggatc
      601 gttggcgacg gaggccagat ggatgatggc atcgacgccg gtgaggtcga tgtcctcggg
      661 cttgcgcacg tcgccgcgaa cgaccctcaa atcggggtgc ggcacgtgct cgttgccgaa
      721 ccactgcgta tcgagcgcca ccacctcatg tccggcgcgg agcagcttcg gcacgagcac
      781 gctgcccttg tagccgcagg cgcctgtcac aagcagtttc atctgatcgc cccttgctcg
      841 acagtgccgc agcctgcgac cggaatcgac tgccggattg ccctgtgtgc ggtagtccgg
      901 tcgcgagccg ggcaagagtg tacgctccca ctacggaatc tcgtgcttcg gcgcactcca
      961 ctgcgagcgg gtgtagtgcg cgtgcaaaac gagcgcaagc ccgagggcca accctgtgct
     1021 ctttcgtcat cgggacaatt gcggcggcgt gaactcgcgg ttcgcgcgcg cgaggctctc
     1081 gagcgtgccg atatcgcgat gatagccctt cgtcacgaca gcagcgatcc gcccgatgta
     1141 gtgcggcaga acctcagtgg aaagatcgac aaccggtcgc cccttcgcgg cgatgaagtc
     1201 gatgacctcc ggctccatga tgtacaccgc cgcgttggcg agatcacctg gcgggctctc
     1261 gaccttttca tgaaaagctt gcacgacgcc ccgatgatcc aactcgagga tgccgcatga
     1321 acgagggtca tcggtgcgaa atgccagcat ggtgatggcg caaccgattg gacgcgagct
     1381 gtgcgccgca atcagtgccg caacattgaa gtccgtgagg ttatccgcat gcgccacaag
     1441 gaacgggccg ccagcgagcc actgccgatt ggccaggatc gttcctcctg ttccaagcag
     1501 ctctgtctca tgcacgaggt ccacgcgatc ccgccaggga gaagcaccaa catgcgcgac
     1561 cacccgctcc gcgagccagt ggcaattgac ggccgcacgg tcaatgcccg ctccgaagag
     1621 gaggtcgagc cagtagtcga ggagcggccg gccgtgcacg gcgacaaggc acttgggcac
     1681 ggacaatgtc agaggtcgca acctggtacc catgcccgcg gcaaggagca atgctctcac
     1741 tggtgacgtc ctccgttgcc aggccgcagc tagcgtgccg cgtaggatcc gcaggtcctg
     1801 ccgatgcgtt cgaaatcgcg agctgcacct gccctacgca cttcgcccaa gcgcagttgg
     1861 cctgcgtcac cggaaaacgc aagaggcgca gcgcatcgtt gacgggatgt cttgctcgaa
     1921 ccattcaccg ccgcgcggat cgtgatcgct ccgggtaggg ccgatccggc aggaggatgg
     1981 cggaaagcgt agcattggcc tacgnnnnnn nnnnnnnncc cgcctgcgca cattcgggcg
     2041 tcgcccttga gcgaggcgag gccggcatcc agtcgcgcat tggctcgcga tgcgagcgcc
     2101 tcagggccgc atgcagcccg ccagcaccac cgttgagccg acccggatcg aatgcgtgct
     2161 ggaccgatcg caccgcggcg cggtgacgac caccaatgcg tagaccaagc aaagtacggc
     2221 aagcatggcc gcaaccccga ccccgactct cgacgccgcg gttatctccg gctgccaggc
     2281 ccgccgcggt ccttcatcga gtggcgcccg ggcgctccca gtgatcacga catccaagcg
     2341 tccgagcttc gccgccataa cccgccccgc aacaaaacgt gtgttgcagg atcttcctca
     2401 accatagatt acatttcaat taaaggttgc agattatcaa caatcttatg ctgttgattg
     2461 caggccccct caggcgttcc caagctccac caacaggtcg gcgagcttca gcggcgcgtt
     2521 gccgacgcgg cccgtctggc aggcggccgc gatcgagccg agatagacgc tctcccagat
     2581 gccggcgccg gcgcagagcg cgagcgaggt cgccgtgaac aggctgtcgc ccgcgcccgc
     2641 cacgtccttc ggcgccttgt tcatcgctgg caacttgtct gtgtagtagg tgccctggtc
     2701 cttgccgtgc acgagcaggc cctcggagcc gagtgtgatg atcaccgcct cggcctccgc
     2761 ctgctgcacg agcccgtttg cgagtacagc gaggccggac gacgtatcac ggagcgcaag
     2821 gcgcgcctca cgctccgtcg gcgtgatgat gctcatgccc ttgaagcgcg agatgtccga
     2881 gatctgcgag gaggcctggc tgtcggccgc catcgggatc ttgcgcgccc tcgcccgctc
     2941 cacgaccgcc tcgacgaggg gctgcggcag gcagccatag ttgaaatccg agaagagaat
     3001 gaggtccgta tgcggcaacc gctcctcgat ctgggcgagc atgttgcgcc acagcgcctg
     3061 gctcacggcg tgctggcgca ggtgattgac gcgcagcagc gtcttgccgg cggcccggta
     3121 gcgctgcttg cgcgtcgttg gccgcgtatc gtccgtgaag gcgtgcacgc cgatgccctg
     3181 cgcctgcaga ttgcgatagg cgaacgccga ggcgtcgtcg gcgcccacca ccgtgaaaaa
     3241 ctggacactc gccccgaggc ctcgcgcatg cgccgagacg acgcccgcgc cgccgacgaa
     3301 cgtcttcgtc tcgatcggcg tcacgacgat ggtcgggtcc tcctgtgaca tgccgagcgg
     3361 atcgcaggtg atgtaatcgt cgatgatgag gtcgccgatt acgagaacac ggatgccctc
     3421 gaccttcttg agccacgtcc tcaacccgtc gagcgtgaag ccatggcgct gcggatattc
     3481 cgacggcttc acgatcgtcg agcggttggt gtcggcgaat tcctcgtcga gcagcgagta
     3541 ggagacgaac tgcgtctcgc ctgagccgaa gacgagcctc ccgccatacg aatcgacgac
     3601 cttctgctcc gggttctcgc gtccctcgaa ctcgcggccc ttgacgacga tcgccggctt
     3661 cagcttggcg atgaagctcg gcgcaggctc gtcgagcagc acggcgtact tgacgagtga
     3721 gagctcgcga atgttggcga ggcgctccgg gcccggcacg gtcacgcccg gggtcgtgtc
     3781 catgttgacg ccgacgaaca ggaaatcgct gagctcggcg gcgaatttga ggagccgcat
     3841 gtggcccgga tgcaggacgt tgaaattgcc ggagacgaag cagatccgct tgtcgcgccc
     3901 cacttctgaa atgacgctct cgtatagtcc gctgtctgcc gtcatgggtc ctgcttaaac
     3961 cggttcggca agcccgcgct cagcacggct cgccacatgt tgcgatcgac tgctcgacag
     4021 acgccacgcg gggccggaat cacgcccccc ctcgagaact ccaccacgct atggcctcat
     4081 cgaggccagc gtcaatcgag cgggccggcg accaaccgac ctcgaccccg agccgacgca
     4141 cgtccgggat gagaaccggc ggatcgtcgg ggcgcatcgg cagcgcgccg agcttcacga
     4201 gcgaccgcgc ctcgagccta tcggcgatcc gcatgacgac gtcgcggagc gcccgcggct
     4261 cgccacaacc gatgttgacg gcgcctccga cagggcttgc cgcaagtgcc gcgatggcat
     4321 caccgcagtc ggcaacgtgc aggaaatctc ggacctgcgt gcccgccgtg cagggtgcct
     4381 cgcgccttgc ttgcagcgcc cgaatgacac tcgagacgag gcgcgcgggc ggctcgccct
     4441 ggccgaaggg atagaagatc cgcgcccacg cgaacgacag tccgtggttc gccgcatagg
     4501 cctcggccat gcgcatgagc gccaccttgc acacgccgta cgggtggccg ggcacgagcg
     4561 gcgtcacgcc ttccgcgcaa aacccatggc gccaatcgta ctcggcgcag ctgccgacgc
     4621 cgacgaagcg cttgccgccg gtcgacgcga acgcctcgat cagctcgagc gacgccgcga
     4681 cccagcgata attgaggtcc gacgtccaga acgcgccatg ccccgtgaac caggccgcat
     4741 gcacgagaag ctcgggccgc accgtcgtca tcagctcagc cgcaaccccc cgttgcatga
     4801 ggtcggcgag atgccatgta acgccggcaa gcgtcaggtc ggggtccgcg gacgtcgcgc
     4861 cgtgcacatc gtgaccgcgc ttcaccagcc cttcggcgat ccagcgaccg acgaagccgc
     4921 gcgcgcccgt gaccagcacc cgcgtcatgg tacgaaatcc gggtacgtgc ggtcgcgctc
     4981 agacatgatg cgcgccggcg cctcgggcca cgcaatcgcg aaggccggat cgtcccagcg
     5041 cacgccgcga cctgcccccg gcacatagaa ggcggagatg tgataggtca cctctgcgtt
     5101 atcggtgagc gtctggaagc cgtgcgcgaa tccctccgga atgtagagcg cccgcccact
     5161 ctcggccgtg aggtccaccg cgaaccactt gcaatatgtg cggctctccg tgcgcagatc
     5221 gacgatcaca tcgcggaccg accccgccgt acagcgcacg agcttgatct cggcgtgcgg
     5281 cgcctcctgg tagtgcatgc cccgcaacgt gtgccgcgtg gcattgcgcg atacgctcca
     5341 ctgcggaaag cgcgtcgcca gtcccgcggc cgcgaactcg cgctcacaga acgagcgcgc
     5401 gaaggagccg cgctcatcgg cgatgggctc caactcgatg acgtaggcac ccgcgagcgg
     5461 cgtctccgcg atcttcacgc gatcacctcg accttcggca gcggtaccac gaacttgccg
     5521 ccccagccgc ggatatgccc catgctcgag acgatctcgt cgcggaggtt ccacggcagg
     5581 attagcagat agtcgggcct ggcggaatcg atcgcctcgg gtgccttcac tgggatgcgt
     5641 gtccccggca acaggcggcc ctgcttggcc gggctccggt cgacgacgaa atcgatgaag
     5701 tcctggcgga tgccgcagta gttgagcagt gtgttgccct tggctgcggc gccatagccg
     5761 acaacgctct tgccctcgcg cttcaccttg atcaggaact cgaggaggct gcgtttcacc
     5821 tcctggacgc gggcgtcgaa gccctcatat ccggcgaccg acccaagccc cgcggcccgc
     5881 tcacgctcga tcagggccgg cacgcgagcg gagcgctcag gcgtcttcgc ctcggcatgg
     5941 cgcgcataga tgcgcagcga gccaccgtgc gtcgcgatct catcgacatc gaacacggtg
     6001 agcccatggt gtgcgaaaat ccgctccacc gcgatcagcg acaggtacga atagtgctcg
     6061 tgatagatgg tgtcgaactg gatgcccgcg atcagcttgg cgagatgcgg gaactccatg
     6121 gtgacgacgc ctgtgggctt cagcgcgagc ttcatgccgg cgacgaagtc gttgatgtcg
     6181 ggcacgtggg ccagcacgtt gttgccgagc agcaggtcag ccttgcgacc atcacgcgcg
     6241 agctcacccg caagctcgcg accgaagaag cggacgagcg tcggcacgcc cttctccacg
     6301 gccgccttcg ccgtattggc cgtcggctcg acgccaagca cggggatgcc catcgccttg
     6361 aaatactgga ggagatagcc gtcgttgctg gcgatctcga tgacctgcga cttcggcccg
     6421 aggccgaagc gtttcgctgc catctcgcaa taggccttgg agtgcgcgag ccagctcgtc
     6481 gagaacgacg agaagtaggc gtagtcgtcg gtgaagatct ggtccgccgc cttgtgctcg
     6541 ggcagctgca cgagcaggca cttgccgcat acgtagacgt tcagcgcata gaagggctcc
     6601 gggtggagcg cgttctcagg cctgatgaac gaattcgacg cgggcgaggt gccgaggtcg
     6661 cagaaagatt gcgcgagcag gcccgagcag aagcggcaga cgtgtgtggt catcagggag
     6721 cccccatagc ctgaaagcgc gcgatctggt cgagtgtgac ggcgcgcatg tccttcccat
     6781 ccctctcggc acggtgccaa tcgatgatcc agtcgaggcc ctggtcgagg ggaagccgcg
     6841 gccgccagcc gagcgccgcg cgcgccttcg aggcatcgag cctcaagaga tgtgcctcat
     6901 gcggtccctg ctgcccgtcg cgcacccacg tggcgccgtc gccccagcgc gccgccatgc
     6961 gatcggcgat ctcgccgact gggatcgcgt catcgggcgc ggggccgaag ttccacccct
     7021 cggcatagcg cgccggctcg ccgtgaagct tctcggcaag cgtaaagtag ccgacgagtg
     7081 cctcgagcac gtgctgccag ggcctcaccg acttcggctg acggatgagt acgggattgc
     7141 ccgacgcgaa gccccggaca agatcgacga tgagccggtc ctcggaccag tcgccgccac
     7201 cgatcacatt gccggcgcgc gcgctcgcca cgccgacgcc cttttcggcg agaaacgact
     7261 tccggtaggc ggccgtcacg agctccgcgc agcccttgct gttggagtac gggtcatacc
     7321 cgcccatggc ctcattctcg cgatagggcc acacccattc gcggttctcg tagcacttgt
     7381 cggatgtcac cacgacgacg gcgcgcgccg gggaaagccg gcggacggca tcgagcagat
     7441 gcacagtccc catcacgttg gtcgcgtagg tagcgacggg atcacggtag ctccgccgca
     7501 ccagcgcctg cgccgccatg tgcagcacga tctccggctc ggcgctcgac accgcggcat
     7561 cgaccgcggc cggatcgcgc aagtccgcga tgatcgaggc catggtctca ccgacgcggg
     7621 ccacccgaaa gaggctcggc tccgtctccg gcgcgagcga gaagccggtc acccgcgccc
     7681 cgagcgagga gagccagagc gccgcccagg ctcccttgaa gccggtgtgc cccgtcagca
     7741 ggacgcgctt tcctttccag aatgccggcg tcatttccag acccgccagg gcgccttgcc
     7801 ggtcgtccag agttcctcga ggtagttctt gtcgcggagc gtatcgagcg ggtgccagaa
     7861 gcccgaatgc tggaaggcgg cgagctggcc ttcagctgcc agccgttcga gcggctcccg
     7921 ctcccaggtc gtgctgtcgg ccacgatgta gtcgatcacc ttcggcgaga ggacgaagaa
     7981 accgccgttg atccaggagc cgtcgccgcg cggcttttcg acgaagcgac gcacgacgcc
     8041 gctgtcgatg tcagcggcgc cgaagcggcc gggcggctgt gtcgccgtca tggtggcgag
     8101 cttcccggag gcgcgatggt gcgcgatgag cttggcgatg tccacgtcgg cgacgccatc
     8161 gccgtaggtg aagcagaact cgcgctcgcc cgcaacgtag ctcttcacgc gcctgaggcg
     8221 accgcccgtc atgctgtcgt cgcccgtatt gacgagcgtc acgcgccagg gctcagcctt
     8281 ggcctcgtgc accgtcatcg agttcttctc catgtcgaag gtgacgtccg acatgtgcag
     8341 gaaatagttc gcgaaatact ccttgatgac gtagcccttg tagccgaggc agatcacgaa
     8401 atcattggcg ccgaaggccg agtagatctt catgatgtgc cagaggatcg gccgccctcc
     8461 gacctccacc atgggcttcg gccggacctg cgtttcctcg ctgatgcgcg tgccgagccc
     8521 gccggccagg atgacgactt tcacgcgttt gcccccttgc agacttgacc agcttcgcag
     8581 aactgtccgg cactcctagt ccatcgtgtt ccggcgaaca agcagccctc cacagcctgg
     8641 agcgatctgt gcaccgttgc ttcgtacgga ggcgcgagcg ctgcctgcca ccggcacggt
     8701 catatgattt ttatgctgat gaactgccta cggacgaggc aagtttcgcc gtccagtccg
     8761 ggaatagtgc accagacttg gtcaatctca atggggggac caccgctgat gggcgtttgt
     8821 tacgatttgt tgaatctgtt gttgttgaca agccttggac tctgggcaac cagctcccag
     8881 tatccgaggc cgcgttcggc agtgatgagc tcgcgagaat atgcgataat tgaagaacat
     8941 tggatcgact gccaatagct cggcgattga cgtgcattgt cggatggtgg catggtgcga
     9001 ggtgaggacg ggaccgtcag tcgagatctg cgcagcgaca ggcggaccga taccgtgtcg
     9061 gtcatcctgc cgaactacaa tcatgcccgc tacctcgagc agtcgatctc ggcggtccta
     9121 aggcagacga ggcctgccga gcaggtcgtt atcatcgacg acggctcaac ggatggcagc
     9181 cttcagctga tcgagcgtct cgttgccggc aatccgacgg tcgaggtcat ccggaatgcg
     9241 cgcccctcgg gcgtggtcag cgccatgaac caggggctcg cgctcgcgcg cggtacgctc
     9301 gttgcgatgc tggcggccga cgacatggtc tttccgacct tcttcgaacg ctccacggcc
     9361 ctcctgaacc agcaccctga cgccgccttt tcctgcgcgc tcaccgagat ctgggacgag
     9421 gacggcgagg tgaccggctc gcgcccattc ttgaggcctg cgctccgcga gcgctacttc
     9481 tcaccggacg ctacccgcca gctgctgcgc aagggcgaca actacttcct cgccgcctcg
     9541 agcctcttcc gttactcgaa gatcgccgag atgggcgggt tcaagggctc cctcgcctcg
     9601 gccgcggacg gcttcctgca gcgccgcatg gcggtccgct ggggcttctg cttcattccg
     9661 gcgcccctcg ctgcctggcg gctgcacggc gccaactatt cccaggcctc ggtgacgaac
     9721 cccgagagct tgaacgagat gctcgcgcag atcacacggc agatcaatgc cgagccggcc
     9781 gggctctttc cggatggcta cgctgagctg ttcgagcgcc gcctgcgctt cggtgccgga
     9841 cgcctcattg catcgaggct gccggccgat ccggtcgcga gcgatcagat cgccaagtgc
     9901 atcaaggccg gctcgatcga gcgacgttgg ctcgcgacgc tcgcgcgcac aggacgactg
     9961 agtccgaggc tcatgattgc ctggctcgca ttgcgcaatc ggcccttttc gccctggtgg
    10021 acgctcgccg agagcacctc ccttgccacg ggccgccggc gcggcagctc cagccaataa
    10081 atcagtcccc gcatccggag gccatgagag tttcggggaa agccaacccg gaggatcatg
    10141 ccgtcgtgga caactccccc aaccttcaac tgccctacat ccttgcggcg caggcccaga
    10201 agcacgtcac ccacaacgag gccgttcgtg cgctcgacgc gctcgttcat ctgagcgtgc
    10261 tcgaccggag cctagcgacg ccgccggcca cgcccgcgga cggcgatcgc tacctcgtcg
    10321 ccgcgggcgc gaccggggct tggtccggcg cctccggcag gattgccgcc taccaggacg
    10381 gcgcctgggc cttcctcacg cctaaggaag gcttcatcgc ctggattgcc gacgagaatg
    10441 tcttgcttgc cttcgatggc gcgacctggc agtccgccct cggcagcggc gccgacatca
    10501 ccgccgatac gctcggcatc aatgctacgg ccgacacgac aaaccgtctc tcgctcgcga
    10561 gcccggcgac gctcttcaac catgagggcg ccggccatca gttgaagatc aacaaggcga
    10621 ccgccgccga caccgccagc atcctcgtgc agacgggctt cacgggccgc gccgagatcg
    10681 gcaccgtcgg cgacgatcat ctgcaggtca aagtcagcgc cgacggaatg acgtggctga
    10741 cggcactcct cgtcgacaag acgagcggcc aggtctggct gccctacacg gcgacgggcg
    10801 tcaccagcct cctcctcaac ggcatcgacn nnnnnnnnna acggcgattt ccagatcaat
    10861 cagcggctgt tcgccggggg cgcactcgct gccggagcct acggcttcga ccgctggaag
    10921 ggcggcgcct cgggcgccag ctgctcggtc agcggcatga tcgtcacgct cacgagcggc
    10981 agcctaacgc aggtgatcga agtcgcacag cgcggccatg cgacgctcgc cgcccagacc
    11041 gtcaccgtaa gcgtcgacgc gctttcgggc ggcaatctca cagtcacgct cggatcacag
    11101 accggcacca tcaccgcggg ctcgggccga cggagcacaa cgctcacact cgaggtcggc
    11161 gacaccggta acatcgccct cacgctcgcg cccgtcagtg ctgctgtgac cttctcgcgc
    11221 gtgcgtgccg agctcggacg ccacgcgtcg gcctggctcg cgccgagccg cgacatcgaa
    11281 ctgagcgcct gccagcgcta ctactggcgg gtcggcggcg tcgccttcaa tccgatcggc
    11341 attggccgct tcgcctcgac gacgcagggc gaggtctatc tgcagcttcc aaccgagatg
    11401 cggacgatcc cgaccgtctc gaccaacggc gtcgcgaatt tctcgttcaa cggtgtcgtt
    11461 ctgaccggcc tcacgttgca cgcgggcttc aaccagcggc gcgactttgt cgggctcctc
    11521 tcaactgtcg gctcagctgc cgggacaccg ggagacatgg cggtcctcgt ctcgcaaccg
    11581 gcgcccgcag gcatcatcga attctcggcc gaactctgag cctcgccacg acggccaatc
    11641 agcgccgccg caaagcgcgc atctcgcgct cgatgcgctc atggacggcc ggcaggaggg
    11701 cctcggccga ccgcccctcg ctttccgcca ggatgagttc ggcggcgagc tccacgcctc
    11761 gcggatgcca gtggctatcg tccgacgccc aataccagtc aaaatcgatc ccgtagggta
    11821 gcttggaggt cttcgggaaa tggatgatct tgtgcccttc cgcctccagc acggcgatcc
    11881 cggtcgccaa gagttcgtcg gtcgatggtt gcagatagac gatgaggtcc gatccgaagc
    11941 ttttcagggc cactgacgcc gccctcacga ctgcgatcaa ccggcagttg gccgcgcggg
    12001 cgttcgcgtc ggtgagcgcc tttatcacct cgggacagtt tcgctcggcg acctccttgc
    12061 ttgcggtcgg gtagcggacc tgcccgacgg cccgtggtgc gcgcgaggcc gccgccgcgc
    12121 gcccgtcgtg aatattgcgc gcttcattgt ggcggtgcgt cgccggcggc ggcggcaggt
    12181 cggtcatgcc aagacgcgcc cggatgaact gccagcccgc atttcgcctt acaatctctt
    12241 cgagcatcca gggcgatgcg cgggggctgt ccggcgcgat gacggcaatc gccgccgaga
    12301 tggcgcgata gccgatgccg ggttccgggt cgacgccgat caactgcccc gagacgtcga
    12361 agcgcgaata ggcctgatca ctgtagaatt cgccagcaat ggcctgcagc acgacaaagc
    12421 gtggctgatt ggccttgaac tccggctggt tgaggaagcg tgccagccgg tgtgccgatg
    12481 acccgttgta ggccgggcag cgcgccctgc gctcgagagc ctccgacaag gtctccgact
    12541 gatcgaggct cgcgccgatc acattcgaat cgccgagcgt gacgaagcag tacgtcttga
    12601 cagctgagta cgccgctcga ttgcgatagc cgagctggtc cgtaaaccag cgcgtcgtgc
    12661 gcggcagcgt gatggtcgcg tagcggtgga cgtcgccgta ctcccactgc tgcagtgtgg
    12721 cattcgggaa gaacgcatag cgcgcgttga ggccattctc cgggatgagc gcctcccact
    12781 gccggaatgt gaacgcattt atcggcagcg cgaaatactc gagcacgatg aaggacagga
    12841 agggcgcgac gagagcgacg gcactcgcaa agagcgacca gagcccctca ctcgagcgtg
    12901 cgagcagcat cttcatttct ccaccccccg cactttcgcc gtcgtgccga ggtcgagctt
    12961 gagcgccgcg atgccgccgg tccccggcca gacctcgccc gtcacccggg tgcctcgcca
    13021 acgattgagc cgctcccaga catagcggcg catccagttc gcctcctgcc gcttccccat
    13081 cgctccgacc agccgcgcaa gctccttgtc gagtaacgct ccctgctgcg gggcccacag
    13141 ataggagccc tcagcgaact cgacgatcag gctcacctgg tcgacgcgtc cgccagtgtc
    13201 tcgactgatg cgcaggagtg cgaacgtcgc gcggccctcc cgcgccggcg tcggaaaggg
    13261 cggtcggaac tgcacgatat cggcaaacgg cgcgagggag cggatatcga tctcgagcac
    13321 accgtcgggt cgcgtgacgg ccttgccggc agccggaccg agctccgtca gcggcggcaa
    13381 gggcgcaccg atgcacacgc gatagaggca gtccggcccg cgcatactcg accagccata
    13441 gaggccgagc gtcagggccg cgatggcagt tgtcgtgatc gttctcagca cgccgctacc
    13501 tgcttggccc gggtcagaac tggaaataga tgaactgttg cgaactctgc cagttgccga
    13561 acacgatgat ggcggcggca gccgcatagt acatggtcca gcgcgccgta tcgttttggg
    13621 cgagtgacgc aagcagcttg ccgccccatt cggcccaggc atcgatgacg agcaggatgg
    13681 cgacgaagag cacgcaccac gcgaacgaca tgcgctcgaa gagctcgaag cccttccaga
    13741 gcggcacctg cccgatgctg gcgatcgact gcagtacacc gattgcctca ccgaacgtgc
    13801 gcgcccggaa gaagatccac gcgaagcaga cgacgtggaa ggtcgtgaac atggcgagga
    13861 gccagccgag ctgtccggcg atcgcaccgc gcaggcccgg cccggcggtc gccgccagcg
    13921 gtcgcgggcg cagtttcgcg agaccgttct cgaaaaggta gatcacaccg tgcacaccgc
    13981 cccagatgat gaaattgaca gcggcgccgt gccagagccc cgagagcagg aacacggcca
    14041 tgatgttgag ggcccagcgg tgagcgccca cctggctgcc gcccaagggc ttgtagagat
    14101 agtcgcggaa ccagctcgac agcgagatat gccagcgcgt ccagaactcg cggatcgagg
    14161 tcgaccagta cggccggcgg aaattctcca tgaggtcgat ccccatgatc cgcgagacac
    14221 cccgtgcgat gtccgagtag cccgcgaaat cgcagtagat ctggaaagcg aagcagtaga
    14281 cgccgagcgt cgcggcaagt ggcccgtgct tgccgggctc cgcaaaaacg tgatcggcaa
    14341 acaacgccaa attgtcggcg atgcacacct tcttgaagaa gccgagcagg atcagctgcg
    14401 agccgacctg catggcctcg cgcgacacca atggctcgct gcgcggttcg acgaacacct
    14461 tgcgaatctg gggcagaaga ttggtcgagc gctcgattgg accggcaacg agctgaggaa
    14521 agaacgcctt gaagagcgcg agcacgatga tgttgtgctc ggcagccttg tcgccgcgat
    14581 agacgtcgac gaggtaggag atcgcctgga acacgaagaa cgagatgccg atcggcagca
    14641 ggatgccgag cggcgtgatt gttgcgccgg gcttcacgac cgacagcgca gagttggcga
    14701 gcgagatcgc gaaatccgtg tacttgaaga acgcgagcag cccgagcagc gagacgatgc
    14761 cgccccacag gtagattttc cgccgaccac ggtcggccgc gatgccgatg ccgcacacgt
    14821 agctgatggc gatcgtaagc agaagcagcc agagatgctt cggcacggca tagccgaaga
    14881 agaagaggct cgctccgagc aaaaggtgca ggcggtagga ctgcggcgtg acgatgaaaa
    14941 ggaggtagaa aactgtgaaa aagatcacga actcgagcga cgtgaagacc atgacgtgac
    15001 ttgtgtctcc cccttgccgc gcgccgcgcg gaagcccccg gtcaactctc acactcacaa
    15061 ttcagaccgc aatgcgcaga tcccgaactc agcgcgcctc agacagtacc ggatcgggcc
    15121 gagcgccccg ccaaccctct cggaacgcac aattcctcgc cgaactctcc gttaggagct
    15181 ggcaagaccg gtgtcaacgc agctagcttg gcaagggcgg gcctgttggg cttctgcgca
    15241 gttgcgccaa ggctgccctt ctaagagcgg cttcgccatt gcccagccga gccgcaaccg
    15301 aaaaatgcac gtttcccatg ttgcaactca attcgaagcg cactccacag cccattccgg
    15361 gcgggcgcct ttctcccagt attttccccg ctctgccgtg attgccgctt tcgacgcgca
    15421 cggaaaccaa gtatgagcgc tcatccgagc ggccgacaag gcgctgaacg agattggagg
    15481 gtgccatggc cagcaacgac aattcctctc ccgcaacccc tccgaagccg ccgggctaca
    15541 ccttcctcgc ctcggtcatc cacccgtatg acccgatcgt gaaggccaat atcaatcacc
    15601 tgacgcgtgg ctatttcgac gagcacatcg tcaagggctt catcgagcgc atcgagggcc
    15661 acacgctggt cacctatgac ggcctcatgg agctcgccaa cgaggtgcgc ttctgcgagc
    15721 tgaacaacat cgagggcgac ttcgtcgagc tcggtgccta caagggcggc tgcatcgcca
    15781 tgatggcgct ctgcaacatg cactacggca agaagcggcg catgttgcac gccttcgaca
    15841 gcttcgaagg cctgccgcag ccggtcgcga aagacctcga cgcccacttc gacggcatct
    15901 tcaagctccg cgacgaccag aagcagggca agctccagtc gatcaacgcc gtcgatgcgg
    15961 gcgaggagta cctcaaccag gtcgtcttcg agaaggtgaa gtacccgcgc gagttcgtga
    16021 agatccacaa gggctggttc cagaataccg tccccgtcgc caagcacgag atcgacaaga
    16081 tcgccatcct gcgcctcgac ggcgatctct acgacagcta catggtgccc ctcgagcacc
    16141 tcttcgacaa ggtcgtgccc ggcggtttca tcatcatcga cgactggatc ttcaccggct
    16201 gccgcgaggc catcaaggac tacttcgagc cgcgcggcgg aattccgtat ctccaccacg
    16261 tcgacatcac ggtgcgtgtc atccgcaaga tgtgatgcgg gcgctgctgc cgttgctcag
    16321 ggacgctcga tcgccgtccg gccgccgctc acccggcggc cacgtgcttg ctgcgcgcgg
    16381 ccttgaaccg ggcgagcccg tcggcgctcc acatggttgc ggcatcgtag aactcggcga
    16441 atgacggcac gccctccggc acgacgaccc atgcgacctt cgtgctcgtg aagatgtgcg
    16501 cgcccggtac gaacgggtgc ggggcctcga gtgtgccgac gcgcacaaaa ttgatactcg
    16561 ggccggctcc cggatagttg ctccacagcg ccacgcggca actcgggcag cgccagatcc
    16621 tctgaccctt gccgctgttc gacggcgtca gcacctgctc gggcgcgccg ctcatgacct
    16681 cgacgcgatc cgcctcgatc atggcattga tcgcaaatgc cgagccggtc tcgcgctggc
    16741 accacgtgca atggcagcaa ttgacgaaga tcggcgcagc catgatccga tacgtcacgg
    16801 cgccgcaagt gcagcgtcca gaatgggcct cagcgaccat ttcgagcctc cctcgtcacc
    16861 ccgttcctgt catgccgacg tcgctacccg aagcctcctg acagcacgtt gtcaggagcc
    16921 gccattgcct cctgaccgga tgctgtcagg aggcctggcg catggtcctg gtcagcaccg
    16981 gaacgaccac cggtcgatgc ttccaggaga tgaccatgtc gaccatcgtt gaacgactgt
    17041 accacgcagg atcgaccacg gagacgcagc ggtcccgccg ctcggtcagc gccgccacgc
    17101 gccttgccgg agcatggcag cgctatatcc gccagcgccg gacggagcgc gccctcgaag
    17161 gactgagcga agcgacgctc aaggacatcg gcatccatcg gagcgagatc cgccgggtct
    17221 cgcgcgatgc aatcgaggcg agggactggc gtcacacgca ctgacatcga ctgacaacgg
    17281 tttacaggag agaccatcat gatcaatcac atctcgatcg gcgtcaccga catcgccgcc
    17341 gcccgccgct tctacgatgc cgccctcggc cccctcggct atcgctgcct cagctccggc
    17401 gagacctcac tcggctacgg gaggaccgcg ccggcactct ggctcatcgc cac
//
LOCUS       DAORNH010000059        17449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2464_length_17449_cov_2.642520, whole genome shotgun sequence.
ACCESSION   DAORNH010000059 DAORNH010000000
VERSION     DAORNH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2464_length_17449_cov_2.642520"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PK264_08205"
     CDS             <1..346
                     /locus_tag="PK264_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD75906.1"
                     /translation="ADRTCGVLAYGDLKRVELAMALANAPRLLLMDEPTAGMAPKERS
                     ALMALASRLAHEQGTAVLFTEHDMDVVFGHARHIIVMNDGALIAEGAPEAVRRNRTVR
                     AVYLGQGAVTGH"
     gene            348..1106
                     /locus_tag="PK264_08210"
     CDS             348..1106
                     /locus_tag="PK264_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD75907.1"
                     /translation="MSTTSAIAAVTPAPGPPEAPILAVRNLEAWYGRGQVLFGVGLEI
                     APGEAVVLIGRNGAGKSTTMRAIMRLVPRSAGEVLLDGVPVQRLPTHEIARAGIGYVP
                     EERRIFTELTVDENLEVGRLPAREGHAWTPAELFELFPNLGRMRNRRGGAMSGGEQQM
                     LTIARSLMGNPRCLLLDEPSEGLAPVIIEGIAEAIAALKTRGVALLLSEQNLHFARLI
                     ASRVYVIERGEIRFSGTIAELDANAAIRDQYLAV"
     gene            complement(1118..>2257)
                     /locus_tag="PK264_08215"
     CDS             complement(1118..>2257)
                     /locus_tag="PK264_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD75908.1"
                     /translation="YVDFLLGSGPMLVGHAHPDVVSAVAAQVARGSTFFFNNAHAIEL
                     AEMISGAVKCAEKLRYTSSGTEATHYAMRLARAYRRRDKIMKFEGGFHGMGDWALMSV
                     TPRHMGNFPQAATDSPGIPQSLTGEMIIAPFNDPEAAASLIRQHKDELAGVIVEPCQR
                     LLPPKPGFLERLRAETRDHGIPLIFDEIVTGFRFAWGGAQEYYGVDPDMAALGKVIGG
                     GHPLAAVAGRDEIMRHFDKGAVGDDGFTQQTGTLSGNPIASVAGLATLEVLKKPGAYE
                     TIFANGRRMMDGLNRVIRSTGTKAQLVGEPPMFDIVFVEGEVSNYRDIARSDGAKLKR
                     MNRLLLERGVIKGEGKLYISLAHTSEDIDQAIAAFEDVLGEMRKG"
     assembly_gap    2258..2330
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2777..2995
                     /locus_tag="PK264_08220"
     CDS             2777..2995
                     /locus_tag="PK264_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75909.1"
                     /translation="MAIWLFIGIVFAAVLLLALVALWTDHNRTTSTLGDQSADATEQR
                     RRADVVAADAARTADARLPPHQGGGLSF"
     gene            complement(3111..3980)
                     /locus_tag="PK264_08225"
     CDS             complement(3111..3980)
                     /locus_tag="PK264_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD75910.1"
                     /translation="MPKILTRSQIEAYNRDGFVFPVRVMSESEARELRAALETYEAKT
                     GGPLRGDLRHKTHLLFTWLADLVRRREIVDAIEDLYGENILCWSTNFFIKEANNPSFV
                     SWHQDATYWGLSSPDVVTAWVALTEATAANGAMDMIPGTHMLDQIPHRDTFAKDNLLT
                     RGQEIMVDVDGAKGVKVLLSPGEMSLHHVRIIHGSAPNTTNDRRIGIAIRYIPTRVRQ
                     LHGNDSATLVRGVDTYKTFEHEPRPAADMEPRMLAMHKAIAERNAQILFRGTGVRDFN
                     DPKALADNRGGGM"
     gene            complement(4049..4501)
                     /locus_tag="PK264_08230"
     CDS             complement(4049..4501)
                     /locus_tag="PK264_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRD75911.1"
                     /translation="MFELDAVTVGLVGTARIVVAAEHTAARVGSGVIAVLATPVMINL
                     IEAAALAAAEHLLPPGHQSLGTRLDVSHTAATPIGGLVTATARVTAVAGREVTFAVEA
                     RDDLEVIAQGVHTRVVVNVEKFENRVRRKANNLEAVLGASLGRGARTT"
     gene            complement(4548..5078)
                     /locus_tag="PK264_08235"
     CDS             complement(4548..5078)
                     /locus_tag="PK264_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD75912.1"
                     /translation="MTVDEFLAWAEGQDGRWELYNGVPYLMSPERTRHSKVKFAAQTA
                     LFNAIRRNGVPCHMLPDGATVRVAEDAAHEPDALVYCGAELPDDAVEVPNPTIVVEVS
                     SPSTRRIDASLKLKGYFAVPSIQHYLIIDPDGPPVVHHKRQDDLTILTRIVHDGALRL
                     DPPGLEVAVADLFEVR"
     gene            complement(5141..5353)
                     /locus_tag="PK264_08240"
     CDS             complement(5141..5353)
                     /locus_tag="PK264_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75913.1"
                     /translation="MPSDVKAIVSALTALVAVGFAFWERAYGQAHLFWLVLGLGAFAI
                     VAMWVFPEATMTKADMPPKPKPGREA"
     gene            complement(5365..6120)
                     /locus_tag="PK264_08245"
     CDS             complement(5365..6120)
                     /locus_tag="PK264_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRD75914.1"
                     /translation="MRIAVISDIHGNLPALEAVLDDIRLRSADLTVNLGDCCASPLWP
                     RETFELLQSLGLPTVRGNHDRWMGERPRSALSRTLNYEYDQLLPQQRAALGVLPATLE
                     FDHGSILAVHGTPASDETFLLEDRVDSRMHLSTPAQIKARLGHVGAGLVLCGHSHTQQ
                     LVHLPAGPVIVNPGSVGLPNYADGPDAPGANARSPHARYAIVTRRANRWSADLIALAY
                     DWDRAARRALDNDRPEWARVYLTGSVLDPETLN"
     gene            complement(6127..6426)
                     /locus_tag="PK264_08250"
     CDS             complement(6127..6426)
                     /locus_tag="PK264_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HRD75915.1"
                     /translation="MLVGIWLLPGLGCDGALAESARSFTREVNIDRSGNDIERVEMDP
                     SAGIDACEQLCRSNAACVAFTYVKQSTTVPRPICRLKDQKPFGHQSSCCVSGVLK"
     gene            complement(6476..7246)
                     /locus_tag="PK264_08255"
     CDS             complement(6476..7246)
                     /locus_tag="PK264_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRD75916.1"
                     /translation="MITERQKMFRQEYRSRIMGFYEGHLHIVIIYVMGAAAFYIYLQH
                     LAAPTWLELVTIPLTFLFTNIFEWATHKYIMHRPIPIKGLRAVYERHTLNHHQFFTDD
                     EMRFRDHKDWRVTVFPPYALVVFILMSIPGAMILGTLFSPNVGWLFMSTTTGMYLIYE
                     FMHFCCHVDENWFVRNCPFVNTLRRHHTAHHNARLMMETNMNLTFPIADWLFGTSDLD
                     RGLVGHLLNGYDDTHLKSSLRGRPKRPDEAAAAPMGNY"
     gene            complement(7503..9404)
                     /locus_tag="PK264_08260"
     CDS             complement(7503..9404)
                     /locus_tag="PK264_08260"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD75917.1"
                     /translation="MKNSRIVLIDRHPGRSSQTIGLAKELGTDPDLIHEPSVGVVGTK
                     GDSQCYIGVLDKVEAIHEALKAKIGKGKGLLKLRLVQPEYTIATSDGIRNGTREMRYS
                     LIGREVTNDALCEHLEATGLAGTIAVVACDKPPVGTLAALLEHNEPAIIMSDGPIHPG
                     HDPKTGETIDLVTAYQVAGHPDEKHRHHIACNACPGIGSCGGMFTYNTMQTFIGVVGM
                     QPLHMVAPPSDDPRRLKEFPAELVKHLANLMAKGLKPRDIVGRDALRNAMIVSMAIGG
                     STNVVLHAPEIARAAGYADFWKEIMTPAEFNDLSKNLVPVLTDARPYGKYSMVDIDKV
                     GGVQVIVRELLEAGLLNGDVMTCTGETLAEQVKRLRTRKPDGKVIYPIAKPYKPTGGL
                     RMLGGNLSPDFSAILKLAGVEGGLSTGGACAGDGPAATTTQANVFRGRACVFENEAAL
                     LDALDKTPERFQNNDIVVVRYEGPSGAPGMPEFLDPTSRITALCRERGITIALMTDAR
                     FSGGSVGLVIGHVGPEAALGGPIAFIEDGDEIVADLNKNELNCPALDNPATLKKRKAA
                     WDKAVKDNGGIHPNCGMADTRLLQRARHMAVPATRGGGLHPNREVWVRDPRKAEKSGF
                     KPTNKHRSG"
     gene            9654..10823
                     /gene="alr"
                     /locus_tag="PK264_08265"
     CDS             9654..10823
                     /gene="alr"
                     /locus_tag="PK264_08265"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRD75918.1"
                     /translation="MTSTGMRPAAAIPPRAAIPLEARAVLTIGLKALRANWAELNRVS
                     GRAECAGVIKADAYGLGLEEISRALTREGCRTFFTATIDEARRVRAVQPGAAIYVLDG
                     LLPGAAPYYAGFDLRPCLSSLGEVREWAAFCRATGRRLRAAVHIDTGMNRLGLPEAEA
                     HELAGDSTLFADFELALVMSHLACADEPANPMNACQKQTFDRLRALLPAAPASLANSG
                     GTFLAPDYHYDLVRPGIALYGGRALEGRANPMQWVVRLAARILQVRDAPAGETVGYGA
                     SYRLERPSRIATLACGYADGFLRALSASTGQHGPVGFIGARAVPVIGRVSMDLITVDV
                     TDVPPGEAIRGAWIEVLGPRTTIDDLTDRAGTIGYELLTRLGRRVHRIYDDGETS"
     gene            10829..12556
                     /locus_tag="PK264_08270"
     CDS             10829..12556
                     /locus_tag="PK264_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRD75919.1"
                     /translation="MASLSEEYSVVIGEFDDVPILLLLADRKMNDNRSYWESLLPMSW
                     ITLRSGYAADTFLRDAIAGLTVAILALPLSIAIAIGSGSKPEAGLITSVVAGFLISAF
                     GGSRYQVGGPAAAFIVIVAAITEKFGTGGLLTATFLAGLILLIAGALKLGTLVRYVPN
                     SVILGFTSGVGCLILVSQVKDFFGLAGKMPSEMIEKLEALWAAKGSARLAPALIGTVT
                     LVLIIALRRMAPRWPGILVAVVAGSALVALLGLNVETVSAIPTRLPDPLLPDLSRATI
                     VAVLPTAATLAFLVGVESLLSAVAADSITGTRHRSNAEIIGQGLANVASPLFGGMPAT
                     GVIARTGTNIQAGARTPVAGMLHAVFVLAFMLLLAPLVAYLAMPCMAAVLVSVAWRLV
                     DVAGVRHYLTRAPLDDRIVMLATLLLTIFVDLNVAIAVGVVSASLLFMHRLAVAQLEH
                     RPTFEGTLLPGGIRLVEIRGPFFYGVASDLVARERARVEWSRVLILDMTAVTHIDASG
                     IEALDELGAECLRRGGRLIVAGLGRFERRALNRMGVVRERRILLARDREHALAVARRN
                     VDAAPTATA"
     gene            complement(12540..13796)
                     /locus_tag="PK264_08275"
     CDS             complement(12540..13796)
                     /locus_tag="PK264_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRD75920.1"
                     /translation="MPVPANIAKLWRAQQGSVAIIFSASVFLVAMSVGLAIDGARTYG
                     VANHLEAALDSAALAGGKMLDDDNISDEDISQRVKAYLNAHIQSQPSEGVTLSLPTID
                     IDRQKGVVAVSVKAAVATTFGKIAGIPSIDFDKSASIVYIAKRIELAMVVDVTGSMNS
                     SGKIDALKSAAREVVDTLLSGNPSPGTIRIGLAPYSAAVNVGPYASTVSGGASVDGCV
                     FERDGSTAYTEAPPSSQPLAAMANPAAPSNPNYGCPAATLLPLTDDRNLLRATIDSYS
                     ASGWTAGHIGAAWGWYLLSSQWSGVWPAASMPRSYSDPKVIKSVLFMTDGEFNTSYNN
                     GNMNATGEGVPGSSAYQALQLCQNMKAQNIVIYSVAFQSPPEAESVLRQCATSPGHFF
                     IAGGNAELRAAFRQIADRIGALRLSR"
     gene            complement(13841..14431)
                     /locus_tag="PK264_08280"
     CDS             complement(13841..14431)
                     /locus_tag="PK264_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75921.1"
                     /translation="MEREVLRLVGAGLVVLVDCVGNAEPNDLASVEDYATILISRAVE
                     ADTPGLSQVTLAAVEKIWAAIRLLGVPAGDVDRYLRAVPTILDAHRPCVASCQGAFKA
                     LRLGQIGDKVETAPSFLLASEVVSSAKRADTLARLKVDGGITLALLERLFAILLPERL
                     LIAALVPLLRPASWMNADRDNFIGEEGVELEGMYAR"
     gene            complement(14587..>15125)
                     /locus_tag="PK264_08285"
     CDS             complement(14587..>15125)
                     /locus_tag="PK264_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRD75922.1"
                     /translation="RQSLATWEAVNSAIGQRDGGKFRRFAQAVTLDELVRLANVELGA
                     LTPRYRLQRSRAAALSLEIVDRDMGDEMRGIRSLSGGERFLVSLALALALSGLYGRRS
                     SVDTLFIDEGFGTLDRETLDMAIDALETLQGQGRRVGVITHVSAMIERIGVRIVVEKR
                     GGGRSVIQVADDASASRA"
     assembly_gap    15126..15135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15136..15466)
                     /locus_tag="PK264_08290"
     CDS             complement(<15136..15466)
                     /locus_tag="PK264_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75923.1"
                     /translation="MLAIDEVRARQLLEVPQERHIALRKSIAEIERRLASAGSRHAAR
                     RQDLAALLGDGAMPAPDAADRLAATIATLSAEHELSAAEIGRIDGELERDVRAREKAT
                     AIEAEIAA"
     gene            15491..>15837
                     /locus_tag="PK264_08295"
     CDS             15491..>15837
                     /locus_tag="PK264_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75924.1"
                     /translation="MRRLPLLFEPPQLRVRSSSLRDAIGLAALAFREPASGRLGQPAR
                     ITRRCLVTLTVCDCFFPAEQRRPLGSDAGVLPGLLTLLRSKLFLISPRRFRLEARGQQ
                     QALVVHQQQPGAGG"
     assembly_gap    15838..15847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15848..16372)
                     /locus_tag="PK264_08300"
     CDS             complement(<15848..16372)
                     /locus_tag="PK264_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75925.1"
                     /translation="MRAASETEARERARQHSLDVELAECLGELVETARRVSLDVGLAT
                     GIEGLREADLQRISVAANCERAAIRDKLATGEALLAEIDRLERQLSLIETDLARLRLA
                     AEEDRQLIGEARERQAREEAARDARLGRSTAIVAELKPYLTAAGLTCEDAERDPRGMM
                     ARLETLAAEFERSDT"
     assembly_gap    17124..17189
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17190..>17449)
                     /locus_tag="PK264_08305"
     CDS             complement(<17190..>17449)
                     /locus_tag="PK264_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75926.1"
                     /translation="VEPVRALLADADRLRRDVERADVALARVTEQFEAARLALDEARA
                     QSEEAERRRTACEADVARFEPQWADASRLDMRVAAAAAELAG"
BASE COUNT         2742 a         5857 c         5584 g         3107 t
ORIGIN      
        1 ggcggatcgc acatgcggcg ttctcgccta cggcgacctc aagcgcgtcg agctcgccat
       61 ggcgctcgcc aatgcgccgc ggctcctgct gatggacgag ccgaccgcgg gcatggcgcc
      121 caaggagcgc agcgcgctga tggcgctcgc gtcccgcctc gcgcatgagc aggggacggc
      181 agtgctcttc accgagcacg acatggacgt cgttttcggc catgcgcggc acatcatcgt
      241 catgaacgac ggcgcgctga ttgcggaagg cgcgcccgag gcggtgcggc ggaaccgtac
      301 tgtccgggcc gtgtacctcg gccagggcgc ggtcacgggg cattgatatg tcgacgacgt
      361 ccgcaatcgc cgccgtgacc ccggcgcccg gcccaccgga agcgccgata ctcgccgtca
      421 ggaacctaga ggcctggtat ggccgcgggc aggtgctgtt cggcgtcggg ctcgagatcg
      481 cacccggcga agcggtcgtg ctgatcgggc ggaacggagc cggcaagtcg accaccatgc
      541 gcgcgatcat gcgcctcgtg ccgcgcagcg caggcgaagt gctgctcgat ggtgtgcccg
      601 tgcagcgcct gccgacgcac gagatcgccc gcgccggcat cggctatgtg ccggaggagc
      661 gtcgcatctt cacggagctc acggtcgacg agaacctcga ggtcggccgc ctgccggcgc
      721 gcgagggcca cgcctggacg ccggccgagc tcttcgagct cttccccaat ctcggccgca
      781 tgcgcaatcg ccgcggcggc gcgatgtcgg gcggcgagca gcagatgctg accattgccc
      841 gctcgctgat gggcaatccg cgctgcctcc tcctcgacga gccctccgag ggtctcgcgc
      901 cagtcatcat cgaggggatc gccgaggcga tcgctgcgct caagacgcgc ggcgtggcgc
      961 tgctcctctc cgagcagaac ctccacttcg cgcgcctcat cgcgagccgc gtctacgtca
     1021 tcgagcgggg cgagatccgc ttcagcggaa ccattgccga gctcgacgcc aatgccgcga
     1081 tccgcgacca gtatctcgca gtctgatcgc gccggactca gcccttgcgc atctcgccga
     1141 gcacatcctc gaaggcggcg atcgcctggt cgatgtcctc gctggtatgg gcgagcgaga
     1201 tgtagagctt gccctcgccc ttgatcacgc cacgctcgag gagcaggcgg ttcatgcgct
     1261 tgagcttggc gccgtccgag cgggcgatat cgcggtagtt cgagacctcg ccctccacga
     1321 acacgatatc gaacatcggc ggctcgccga cgagctgcgc cttggtgccc gtcgacctga
     1381 tcacccggtt gaggccgtcc atcatgcgac ggccgttcgc aaagatcgtc tcataggcgc
     1441 cgggcttctt cagcacctcg agagtggcga ggcctgcgac cgaggcgatc ggattgccgg
     1501 agagcgttcc cgtctgctgc gtgaagccat cgtcgccgac ggcgcccttg tcgaaatggc
     1561 gcatgatctc gtcgcgcccc gcgaccgcgg cgagcggatg gccgccgccg atcaccttgc
     1621 cgagcgccgc catgtcagga tcgacgccgt agtactcctg cgcgccaccc caggcgaagc
     1681 ggaagcccgt cacgatctcg tcgaagatga gcgggatgcc gtgatcgcgc gtctcggcgc
     1741 gcagccgctc gaggaagccc ggcttcggcg gcagcaggcg ctggcagggc tcgacgatca
     1801 cgcccgcgag ctcatccttg tgctggcgga tgaggctcgc cgcggcttct ggatcgttga
     1861 agggggcgat gatcatttcg ccggtgagcg actgcggaat gccaggcgaa tccgttgcgg
     1921 cctgcgggaa gtttcccatg tggcgcggcg tgacgctcat cagggcccaa tcgcccatgc
     1981 catggaaacc gccctcgaac ttcatgatct tgtcgcggcg gcgataggcg cgggctagcc
     2041 gcatcgcgta gtgcgtcgcc tcggtgccgc tcgatgtgta gcggagcttc tcggcgcact
     2101 tcacggcgcc cgagatcatt tcggcgagct cgatcgcgtg cgcgttgttg aagaagaacg
     2161 tcgagcctct cgccacctgc gcggccaccg ccgagacgac gtccgggtgc gcatgaccga
     2221 cgagcatcgg gccggagccg agcaggaaat cgacatannn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtgccgccc
     2341 gccggcatca cgcggccggc cttcaggcgc agctcctgct ctcgccggga cggggcttcg
     2401 atgtgaatgt tcgcgctcat gggctcctcg ctcgacgatc gatctcgcgc aagcatgcca
     2461 catctgatcg ggcgactgaa ccgccgccgc tacaggggag caatgccgag gtcggcgagg
     2521 cgggggaatt caaccacgcc caacgagcat cgccaaatca agagttttct gcaacgccgc
     2581 cggagaatcc ggagtgtgtg tctgtgcccg tcaacgaggt cgtagaaacc tcagaattgt
     2641 gagacgtata cagaatagct acattgagcc atcccctgcg gaaggtggtg gttgtctcat
     2701 ccggcttcgg cgctaactgg ctgtcatccg caaccggctc gtgatatggg ttcttttgtc
     2761 agaaatggag gtcgccatgg ctatttggct cttcatcggc attgtgttcg ccgccgttct
     2821 tctgctggcg ctggtagcgc tctggactga ccacaatcgc accacctcaa cgctcggcga
     2881 tcagagcgct gatgctaccg agcaaagacg gcgggcggac gtcgtggcag cggatgccgc
     2941 gcgaactgcg gatgcgcgac tgccaccgca tcagggcggc ggcctgagct tctgaacgcc
     3001 ccgccgagca ttcacgctct ccaccctgct ccgtgcacgc gattggcaat accgcgatag
     3061 cggtctagtg caaacagact ttcccgacag accgggccaa tgctgcgcgt ctacattccc
     3121 ccaccgcgat tgtcggcgag cgccttcggg tcgttgaagt ccctcacgcc ggtgccgcgg
     3181 aagagaatct gcgcgttgcg ctccgcgatc gccttgtgca tcgcgagcat gcgcggctcc
     3241 atgtccgcgg ccggcctcgg ctcgtgctcg aaggtcttgt aggtgtcgac tccgcgcacc
     3301 agcgtcgcac tgtcgttgcc atggagctgg cggacgcggg tcgggatgta gcggatggcg
     3361 atgccgatgc gccggtcgtt ggtcgtgttg ggcgctgagc cgtggattat gcgcacgtga
     3421 tgaagcgaca tctcgcctgg gctgagcagc accttgacgc ccttcgcgcc gtcgacatcg
     3481 accatgatct cctgtccgcg ggtcaggaga ttgtccttcg caaaggtatc gcggtgcggg
     3541 atctggtcga gcatgtgcgt gcccgggatc atgtccatgg ccccgttggc ggcggtcgcc
     3601 tcggtcagcg ccacccacgc ggtcaccacg tcaggtgagg agaggcccca ataggtcgca
     3661 tcctggtgcc aggacacgaa cgagggattg ttcgcctcct tgatgaagaa gttcgtcgac
     3721 cagcaaagga tgttctcgcc gtagaggtcc tcgatcgcgt cgacgatctc gcgccgccgc
     3781 acaaggtccg cgagccaggt gaacaggaga tgcgttttgt ggcggaggtc gccgcgcagc
     3841 ggaccgcccg tcttcgcctc gtaggtttcg agggccgcgc gcagctcgcg cgcctcgctc
     3901 tcggacatga cgcgaacagg aaagacgaat ccatcgcgat tgtaggcttc gatctgggat
     3961 cgagtgagga tcttgggcat gtcggctccg cacgttgctg ctggtcttca ggcggcgctc
     4021 agggccggcc tcgtcgatgt ctctcgcctc aggtcgtccg cgcgccgcgc ccgaggctcg
     4081 cgccgagcac ggcttcgagg ttgttggcct tgcgccgcac gcggttctcg aatttctcga
     4141 cattgacgac gacgcgggtg tgcactcctt gcgcgatgac ttcgagatca tcgcgcgcct
     4201 cgacggcgaa cgtcacctcg cgtccggcaa cggccgtcac acgggcggtc gccgtcacga
     4261 ggccgccgat cggtgtcgca gccgtatgcg aaacgtcgag gcgcgtcccg agcgactgat
     4321 gccccggtgg caggaggtgc tcggccgcgg ccagcgccgc agcctcgatg agattgatca
     4381 tgacgggtgt cgccagcacc gcgatcacgc ccgagccgac gcgagcggcg gtgtgctcgg
     4441 ccgcaacgac gatgcgcgcg gtgccaacga gaccgaccgt caccgcatcg agttcaaaca
     4501 tgcctcagct ttcctttcca atgttgcggc accggcggat tccttgctca gcggacctcg
     4561 aacaagtcgg cgactgcaac ctcgaggccg ggaggatcga gacggagcgc cccgtcatga
     4621 acgatacgtg tcagaatcgt gaggtcgtcc tgccgcttgt gatgaacgac cggaggcccg
     4681 tcgggatcga tgatcaggta gtgctggatg ctcggcacgg cgaaataacc cttgagcttc
     4741 aaggacgcgt cgatccgtcg tgtcgaggga gacgacacct cgaccacgat cgtcgggttc
     4801 gggacctcga ctgcatcgtc cggcagctcg gcgccgcagt atacgagcgc atccggctcg
     4861 tgggctgcat cctcggcaac ccgcaccgtg gcaccgtcag ggagcatatg gcacggcaca
     4921 ccattgcgac ggatggcgtt gaatagcgcc gtctgggccg cgaacttcac cttgctgtgg
     4981 cgcgtccgct cgggtgacat cagataaggc acgccattgt agagttccca acggccgtcc
     5041 tgcccctcgg cccaagccag gaactcgtcg acagtcatgc gtcgcttcac ggcctgcgtc
     5101 atgtggggag cctacacctg aacgttcctc gcggcatgcg tcacgcttcg cggcccggct
     5161 tcggcttcgg cggcatgtcg gccttggtca tggtcgcctc ggggaatacc cacatggcga
     5221 caatggcaaa ggcaccgagg cccaagacca gccagaagag atgcgcctga ccgtaggccc
     5281 gctcccagaa cgcgaagccg acagcaacaa gagccgtcag ggcggaaacg atcgctttga
     5341 cgtcgctggg catgaggaac tcgttcagtt cagagtttct ggatcgagga cggagccggt
     5401 gaggtacacg cgcgcccatt ccggccggtc gttatcgagc gcgcggcgcg cggccctatc
     5461 ccaatcataa gcgagtgcga tcaggtcagc cgaccagcga ttggcgcggc gcgtcacgat
     5521 cgcatagcgc gcgtgcggtg agcgggcgtt ggcgcccggg gcgtctggcc catcggcata
     5581 gttcggcaag ccgacgctgc ccggattgac gatcaccggc cccgccggca aatgcacgag
     5641 ctgctgagtg tggctgtggc cgcagagcac gaggcccgcg ccgacgtgcc cgaggcgtgc
     5701 cttgatctgc gcgggcgtcg ataggtgcat gcgactgtct acgcggtcct cgagcaggaa
     5761 agtctcgtcg ctcgccggcg tgccatgcac cgcgaggatc gagccatggt cgaactcgag
     5821 tgtcgccgga agtactccaa gcgcggcgcg ctgttgcggc aggagctggt cgtattcata
     5881 gttcaacgta cgactgagcg cgctcctcgg ccgctcgccc atccagcgat cgtgattgcc
     5941 ccgcacagtc ggcagtccga gcgactgcag caactcgaac gtctcgcgcg gccacagggg
     6001 gctcgcgcag cagtcgccga ggttcaccgt gagatcggca ctgcgcaggc gtatgtcatc
     6061 gagcaccgct tcgagtgccg ggaggttgcc gtgaatgtcg ctgatgacgg caattcgcat
     6121 cgatcgctac ttcaagacgc cgctgacgca gcagctgctc tggtgaccaa agggcttctg
     6181 atccttcaac cggcagatcg gccgcggaac cgttgtactc tgcttcacat atgtaaaggc
     6241 gacacacgcg gcgttgctac ggcaaagttg ctcgcaggca tcgatgccgg ccgacgggtc
     6301 catttcgaca cgctcgatgt cattgccaga acggtcgatg ttcacttcac gcgtgaagct
     6361 gcgagcactt tcagccagag caccgtcgca tcccagcccc gggagcagcc agatgcctac
     6421 cagcatcact gcccacgaac gattggaccg gtgacgattc ctcatcgggc gcctctcaat
     6481 agttgcccat cggcgccgcg gcggcctcgt cgggacgctt cggccggccg cgcaggctcg
     6541 atttcaggtg cgtgtcgtcg tagccattga ggaggtggcc gacgagcccg cgatcgagat
     6601 cggacgttcc gaacagccag tcggcgatcg ggaaagtgag gttcatgttc gtctccatca
     6661 tcagccgcgc gttgtgatgg gcggtgtgat ggcgccggag cgtgttgacg aacgggcagt
     6721 tcctgacgaa ccagttctca tcaacatggc aacagaagtg catgaactcg tagatcaggt
     6781 acatgcccgt cgtcgtcgac atgaacagcc agccgacgtt cggcgagaag agcgtgccga
     6841 ggatcatcgc acccgggatc gacatcagaa tgaacacgac cagggcgtag ggcggaaata
     6901 cggtgacgcg ccagtccttg tgatcgcgga agcgcatctc atcgtcggtg aagaactgat
     6961 ggtgattgag tgtgtggcgc tcgtagaccg cgcgcagccc cttgatcgga atcggccggt
     7021 gcatgatgta cttgtgcgtc gcccactcaa agatgttcgt gaacaggaac gtcagcggga
     7081 tggtgacgag ttcgagccaa gtcggcgcgg caaggtgctg gaggtagatg tagaacgccg
     7141 ccgcgcccat gacgtagatg atgacgatat gcagatggcc ttcatagaag cccatgatgc
     7201 gcgagcggta ctcctgacgg aacatctttt gccgttcggt gatcatcgat agccctcgtc
     7261 gctgcccttg aacgcccacg cgctccggcc aatgcttgac cagccccatt ggccgccact
     7321 gcaccgccgc aacgccaaac ggcgtaacga tgtgatatat cagagttagc cgatcgtggg
     7381 catgtcgtca atgccggcac ttggtgccgg cgctgtggtt gacggcgcac gtgcgttgat
     7441 gcggttggtg cggcgcggga gtgtcgccgc tcccgtgccg cgcgctcgtc gtgcaacgtg
     7501 cctcagcccg agcgatgctt gttcgtcggc ttgaagcccg acttctctgc tttcctcgga
     7561 tcgcgcaccc agacctcgcg gttcggatgc aggccgccgc cccgcgtcgc cggcaccgcc
     7621 atgtgccgcg cacgctgcaa gaggcgcgta tcggccatcc cgcagttggg gtggatgccg
     7681 ccgttgtcct tcaccgcctt atcccaagcc gccttgcgct tcttcagcgt cgccgggttg
     7741 tcgagcgccg ggcagttgag ctcgttctta ttgagatcgg cgacgatctc gtcgccgtcc
     7801 tcgatgaagg caatggggcc gccgagtgcc gcctccggcc cgacgtggcc gatgacgaga
     7861 ccgactgatc cgccggagaa gcgcgcgtcc gtcatgaggg cgatcgtgat gccgcgctct
     7921 cggcagagag cggtgattcg cgaggtcggg tcgaggaact ccggcatgcc aggtgctccg
     7981 ctaggtccct catagcgcac gacaacgatg tcgttgttct ggaagcgctc gggcgtcttg
     8041 tcgagcgcgt cgagaagagc agcctcgttc tcgaacacgc acgctctgcc gcggaagacg
     8101 ttggcctgtg tggtcgtagc ggcaggacca tctcctgcac acgcccctcc ggtcgacaac
     8161 ccgccttcca cgccggcaag tttcaggatc gccgaaaagt ccggcgacag attgccgcca
     8221 agcatcctga ggccgcccgt cggcttatat ggcttggcga tcgggtagat gactttgccg
     8281 tcgggcttgc gggttcggag gcgcttcacc tgctcggcaa gcgtctcgcc cgtgcaggtc
     8341 atgacatcgc cgttgaggag gcccgcctcc agaagctcgc gcacgatcac ctgcacgccg
     8401 ccgaccttgt cgatgtcgac catcgagtac ttgccgtagg gccgcgcgtc ggtgagcacc
     8461 gggacgaggt tcttcgagag gtcgttgaac tcggccggcg tcatgatctc cttccagaaa
     8521 tcggcgtagc ctgccgcgcg cgcgatctcg ggcgcatgca gtacgacatt ggtcgagccg
     8581 ccgatcgcca tcgagacgat catggcattg cgcagcgcat cgcggccgac gatgtcgcgc
     8641 ggcttgaggc ccttcgccat caggttggcg agatgcttca cgagctcggc cgggaactcc
     8701 ttcaggcggc gcggatcgtc cgacggcggc gccaccatgt ggaggggttg catgccgacg
     8761 acgccgatga acgtttgcat cgtattgtag gtgaacatgc cgccgcagct gccgatgccg
     8821 ggacaggcat tgcaggcgat gtggtggcgg tgcttctcgt ccgggtgccc ggccacctga
     8881 taggcggtga cgaggtcgat cgtctcgccg gtcttcgggt cgtgccccgg atggatcggc
     8941 ccgtccgaca tgatgatcgc gggctcgttg tgctcgagca gtgccgccag cgtgccgacg
     9001 ggcggcttgt cgcaggcgac gacggcgatg gtgccggcaa ggcccgtcgc ctcgaggtgc
     9061 tcgcacagcg catcgttcgt cacctcgcgg ccgatgagcg agtagcgcat ctcgcgcgtg
     9121 ccgttgcgga tgccgtccga tgtcgcgatc gtgtactccg gctgcaccag gcggagcttc
     9181 agcaggcctt tgcctttgcc gattttggcc ttcagcgcct cgtggatcgc ctcgaccttg
     9241 tcgagcacgc cgatgtagca ctggctgtcg cccttggtgc cgacgacgcc gacggagggc
     9301 tcatggatga ggtctgggtc ggtgccgagc tccttggcga ggccgatggt ctgggacgag
     9361 cggccgggat ggcggtcgat caggacgatg cgcgaattct tcaaggggtc tctcctaccg
     9421 cgggcacggt tgccgttgga cgaaatgtgg ggacggccaa tgccgccggt cgcgagctga
     9481 gtactctttc tgtcgggaat tttggcgcgt tgcaagcacg cggcgcgcca ctcagccccg
     9541 ccgaaattcg gctcgctcgc gccgatgcaa tcgccaacca ccgtggctgc tctggtgaaa
     9601 tccggcggca ccggttagag acagcgctcg gcacgagcga agcgaggttg gaaatgacca
     9661 gcacaggcat gcgccccgcg gctgccattc cgccccgcgc cgccattccg ctcgaggcgc
     9721 gcgcagtact gaccatcggt ctcaaggcgt tgcgtgccaa ctgggccgag ctcaaccgtg
     9781 tctcgggccg cgcagagtgc gcaggcgtca tcaaggcgga cgcctatggc ctcgggctcg
     9841 aggagatcag ccgcgcgttg acgcgcgagg gctgtcgcac cttcttcacg gcaaccatcg
     9901 acgaggcgcg acgcgtgcgc gcggtgcaac cgggcgccgc gatctacgtt ctcgacggcc
     9961 tcttgccggg cgccgccccc tactatgccg ggttcgatct ccgcccctgc ctctcgagcc
    10021 tcggagaagt gcgggaatgg gccgccttct gccgggcgac cggacggcgc ctcagagcag
    10081 cggtgcacat cgacaccggc atgaaccgtc tgggtctccc ggaggcagag gcgcatgagc
    10141 ttgcgggaga ttcgactctc tttgccgatt tcgagcttgc gctcgtcatg agccatcttg
    10201 cctgtgcgga cgagcccgca aatcccatga atgcgtgcca gaaacagacg tttgaccgtc
    10261 tgcgcgcgct gctgccggcc gcaccggcga gcctcgccaa ctcgggtggg accttccttg
    10321 cgcccgacta tcactatgac ctcgtcaggc cagggatcgc cctctacggc ggccgcgcgc
    10381 tcgaaggccg cgccaatccg atgcagtggg tcgtacgcct tgcggcacgc atccttcagg
    10441 tccgcgatgc gcctgccggc gagaccgtcg gctatggcgc ctcctatcgg ctcgagcgcc
    10501 cctcgcgcat cgccacactt gcctgcggct acgccgatgg cttcctcaga gccctcagcg
    10561 cctcgacagg gcagcacggc ccggtcggct tcatcggcgc acgcgccgtc cccgtgatcg
    10621 gccgcgtctc catggacctc atcaccgtcg acgtgaccga cgtcccgccg ggcgaggcga
    10681 tccgcggcgc ctggatcgag gttcttggcc cgcgcacgac gatcgatgat ctcaccgacc
    10741 gcgccggcac catcggctac gagcttctca cgcgactcgg ccggcgcgtg catcgcatct
    10801 acgacgatgg cgagacgagc tgacgcactt ggcaagtcta tcggaggagt actctgtcgt
    10861 catcggcgaa ttcgatgatg tgccgattct gctgctgctc gcggatcgca aaatgaacga
    10921 caatcgctca tactgggaaa gcctccttcc catgagctgg atcacgttgc gatccggcta
    10981 cgccgccgat acctttctcc gcgatgccat cgccgggctg acagtcgcga tcctcgcgtt
    11041 gccgctctcg attgcgattg ccatcggctc ggggtccaag cccgaggccg gcctgatcac
    11101 ctcggtcgtg gccggctttc tcatctcagc cttcggcggc agccgctatc aggtcggcgg
    11161 acccgcggcc gcctttatag tcattgtcgc cgccattaca gagaagtttg gcacgggcgg
    11221 gctgctcacc gcgacattcc tcgccggcct catactgctc atcgccggcg ccctgaagct
    11281 cggcacgctc gtgcgctatg tcccgaattc ggtgatcctc ggcttcacca gcggcgtcgg
    11341 ttgcctcatt ctggtgagcc aggtgaagga tttcttcggc cttgccggca agatgccctc
    11401 ggagatgatc gagaagctcg aggcgctctg ggcggccaag ggcagcgccc gcctcgcgcc
    11461 cgcgctgatc ggcactgtga cgctcgtcct gatcatcgcg ctgcgccgca tggctccgcg
    11521 ctggcccggc atcctggtcg ccgtcgttgc gggctcggcg ctcgtcgccc tgctcggcct
    11581 caacgtcgag accgtgagcg cgatcccgac ccgcctcccc gatcctctcc tgcctgatct
    11641 ctcgcgcgcc accatcgtcg cggtgctgcc gaccgcggca acgctcgcct tcctcgttgg
    11701 tgtcgaatcc ctgttgtcgg cggtcgcggc cgattccatc accggcacac gtcatcgctc
    11761 caacgccgag atcatcggcc agggcctcgc caatgtcgcc tcgcccctct tcggcggcat
    11821 gccggcgaca ggtgtgatcg cgcggaccgg caccaacatc caggccggtg cccgcacgcc
    11881 ggtcgccggc atgctgcacg ccgtgttcgt gcttgccttc atgctgctgc ttgccccgct
    11941 cgtcgcctac cttgcgatgc cgtgcatggc ggcggtgctc gtcagcgtcg cctggcgcct
    12001 cgtcgatgtc gccggggtgc gtcactacct cacgcgcgcc ccgctcgatg atcgcatcgt
    12061 catgctggcg acgttgctgc tgacgatctt cgtcgacctc aatgtcgcga tcgccgtcgg
    12121 cgtcgtttcc gcctcgctgc tgttcatgca tcgcctggcg gttgcccagc tcgagcacag
    12181 gccaaccttc gagggcacgt tgctacccgg cggcatccgg ctcgtcgaga tccgcggccc
    12241 ttttttctac ggcgttgcaa gcgacctcgt cgcgcgtgag cgtgcccggg tcgagtggtc
    12301 gcgcgtcctc atcctcgaca tgacggccgt cacccacatc gacgccagcg gcatcgaggc
    12361 gctcgacgaa ctcggcgcgg agtgtctgag acgcggcggc cgcctcattg tcgcagggct
    12421 cggccgcttc gagcggcgcg cgctcaaccg catgggtgtc gttcgcgagc ggcgcatcct
    12481 cctcgcgcgt gaccgcgagc atgcgcttgc cgtagcaagg cgcaacgtcg acgctgcgcc
    12541 taccgcgaca gcctgagcgc tccgatccga tcggcgatct ggcggaaggc ggcccttagc
    12601 tcggcgttgc ccccggcaat gaagaagtgg ccaggcgagg tcgcgcattg gcgcagcacc
    12661 gattctgcct cgggcggaga ctggaaggcg acagaataga tgacgatgtt ctgcgccttc
    12721 atgttctggc agagctggag cgcttgatac gccgaactgc caggcacacc ctcgccggtc
    12781 gcattcatat tgccgttgtt gtagctcgtg ttgaactcgc catcggtcat gaacagcacc
    12841 gacttgatca ccttggggtc cgagtagctc ctcggcatgc tggctgcggg ccagacgccg
    12901 ctccactggc tcgagagcag ataccatccc caggccgcgc cgatgtgacc ggcggtccag
    12961 cccgaggccg agtacgaatc gatcgtcgcc cgcaggaggt tgcgatcatc cgttagcggc
    13021 agcaacgtcg ccgccggaca gccgtagttc ggattgctcg gcgccgcggg gttggccatg
    13081 gccgccagcg gttggctgga cggcggagct tcggtgtaag cggtggagcc gtctcgctcg
    13141 aagacgcagc catctacgct ggccccgccg ctgaccgtac tcgcgtaggg tccgacgttg
    13201 accgcagccg agtacggagc gagcccgatc cggatcgttc ccggagacgg attccccgag
    13261 aggagcgtat ccacgacctc gcgtgccgcc gacttcagcg cgtcgatctt gccgctggag
    13321 ttcatcgacc ccgtgacatc gacgaccatg gcaagctcga tgcgctttgc gatgtagacg
    13381 atcgaggcac tcttgtcgaa atcgatcgag ggaatgcccg cgatcttgcc gaaggtcgtc
    13441 gcaaccgcgg ccttgacgct gacggccact acgcccttct gccgatcgat gtcgatcgtc
    13501 ggcagcgaca aggtaactcc ttcgctcggc tggctctgga tgtgcgcgtt gagataggcc
    13561 ttcacacgct gactgatatc ctcgtcggaa atgttgtcgt cgtcgagcat cttgccgccg
    13621 gcgagcgcgg ccgaatcgag tgccgcctcg agatggtttg cgacgccata agtgcgcgcg
    13681 ccgtcgatcg cgagcccgac cgacatcgca acgagaaata cactcgccga aaagatgatc
    13741 gcgacgcttc cctgctgggc tcgccagagc ttcgcaatgt ttgcaggcac cggcatgact
    13801 tcatccccac gtcggcggca cgaacgccac tccaccgccc tcagcgggcg tacatgccct
    13861 cgagttcgac gccttcctca ccgatgaagt tgtcgcgatc cgcattcatc cagctcgccg
    13921 gtctgagcag cggcaccaga gccgcgatca gcaggcgctc aggcagcagg attgcgaaca
    13981 gccgctcgag cagagcgaga gtgatccccc cgtcgacctt gagccgcgcg agcgtatcgg
    14041 cccgcttggc gctcgacacg acttcggatg ccagcaggaa cgaaggcgca gtctcgacct
    14101 tgtcgccgat ctggccgaga cggagcgcct tgaaggcgcc ctggcacgac gcgacgcacg
    14161 gccgatgggc atcgagaatg gtcggaaccg cccgaagata gcgatcgaca tcccccgccg
    14221 gtacgccgag caatcggatc gccgcccaga tcttttcgac cgcggctagc gtcacctgcg
    14281 aaagccccgg ggtatccgct tcgaccgcac gactgatcag gatcgtcgca taatcttcga
    14341 cggaagccag atcgttgggc tcggcattgc cgacacaatc gacgaggacc acgaggccgg
    14401 ccccgacgag acgcagaact tcccgctcca tgcgaccacc ctccgagacg cgagcacagg
    14461 gtcagccgcc agaggtagca gaggattgct aatcacttat tatcctgcgc ggtttttatg
    14521 ggcttccgag tgcaatcgtt aatttgcgca tgcccacgga gtaaattcag gaatatgagc
    14581 gccgtatcag gcacggcttg ccgaagcatc gtccgccacc tggatgacac tgcgtccgcc
    14641 cccgcgcttc tcgacgacga tcctgacgcc gatccgctcg atcattgccg agacatgcgt
    14701 gatcacgcct acgcgccggc cttgcccctg cagcgtctcg agcgcatcga tcgccatgtc
    14761 gagcgtctca cggtcgagcg tgccgaaacc ttcatctatg aagagcgtgt cgacggacga
    14821 gcggcggccg tagaggccgg agagcgccag tgccagcgcg agcgacacca ggaatcgctc
    14881 cccgccggag agagatcgta tgccgcgcat ctcgtccccc atgtcgcggt cgacaatctc
    14941 gaggctcagc gcagccgcac ggctccgctg cagccggtat ctcggggtca gcgcgccgag
    15001 ctccacgttc gcgagccgga cgagttcgtc gagcgtgacg gcttgagcga aacggcggaa
    15061 cttgccgccg tcccgctggc cgatcgccga gttcaccgcc tcccaggtcg caagggattg
    15121 gcgggnnnnn nnnnnccgcc gcgatttcgg cctcgatcgc tgtcgccttc tctcttgcac
    15181 gcacatcgcg ctcgagctcg ccatcgatgc gcccgatctc ggcggcgctg agttcgtgct
    15241 cggcgctgag cgtagcaatc gtcgccgcga ggcggtcggc tgcgtcgggt gccggcattg
    15301 cgccatcgcc gagcaaggcc gcaaggtcct gccgacgcgc ggcgtgacgg ctccccgcac
    15361 tcgccagccg ccgctcgatc tcggcgatgc tcttcctgag cgcgatgtga cgctcctgcg
    15421 gcacctccag cagctggcgg gcccgcactt cgtcgattgc cagcattgta acggccttgg
    15481 tgagcccggc gtgcgccgtc tcccgctcct tttcgagccg ccgcagctcc gcgtccgcag
    15541 ctcttcgctg cgcgatgcaa tcggtctcgc cgcgttggcc ttccgcgagc ctgcgtccgg
    15601 acgtctcggc caaccggcgc gcatcacgcg ccgctgcctc gtgacgttga cggtgtgtga
    15661 ctgtttcttc cccgccgagc agcgccgccc gctcggatcg gacgctggag tgctgcctgg
    15721 cctgctcact ctcctccgca gcaagctttt cctcatctcc cctcgccgat tccgccttga
    15781 ggctcgcggc cagcagcagg ccctggtggt ccaccagcag cagccgggcg ccgggggnnn
    15841 nnnnnnncgt atcgctgcgc tcgaattcgg cagccagcgt ttcgagccgg gccatcatgc
    15901 cccgcgggtc gcgttccgcg tcctcgcacg tgagccccgc tgccgtgaga tacggcttca
    15961 gctccgcgac gattgccgtc gaccggccga gccgcgcgtc gcgcgccgcc tcctcccggg
    16021 cctgccgctc ccgcgcttca ccgatgagct gcctgtcctc ttccgccgcc aggcgcaggc
    16081 gcgccagatc cgtctcgatg aggctcagct gcctttcgag gcgatcgatc tcggccagca
    16141 acgcctcgcc ggtagcgagc ttgtcgcgga tcgccgcgcg ctcgcagttg gccgcaaccg
    16201 aaatccgttg aaggtcggcc tcacggagcc cttcaatgcc cgtcgccagg ccgacgtcga
    16261 gcgacacccg ccgcgccgtc tcgacgagtt cacccaagca ctcggccagc tcgacatcga
    16321 gactgtgctg ccgggcgcgc tcccgcgcct ccgtttcgga cgccgcccgc atgtccgctc
    16381 gagccgacgc ctcctctcga aatgcacggc tgacctccgc ctccagcacc ttgatcgatt
    16441 tgtcgagagc agtccgtgcc gtgcggatct cgcgtgccag cgccgccgtc gcgtcatgag
    16501 cctcgccggc cggatgctcc gtgcttccgc agaccggaca aggctcgccc tggagcagac
    16561 ggctgcgcag cgccgccgcc tgcgccgagg cggccgcctc ggcgagatcg accagccgct
    16621 gtccgcggtc gcgctcgctg atcagctgct cgaggttgcc ctcagccgtc ttcaggcgct
    16681 gcgctgcctc gctcgatgcg agcttcgccc tccggccgct tgccgatgct tccgcggcgg
    16741 cgtcccgcgc ctcgcgggat cgcttcagga gatcgagaga cgagcgcgcg gtgctcgcca
    16801 gcagttcaag ctgctcaccg cgatcgcgaa gctcgccaaa gcggatcacc tcgttcgccg
    16861 tccgccgggt ggcgatcctg gccttgagcg cttcgcgctc tcgctcccgg ctcgcgatca
    16921 gatcgtcgct ggagcgaatg cgtgtctcga gctcggcgat ctccttcaac cgacgtgcgg
    16981 tctcggacaa cgcggcggcg gtttcggatc tcagcacggc gagatccgca gcccgccgcc
    17041 ggacgatctc gatatgccgc gcaattgccg catgcgccgc gcgctgtgtg cgtgcgcgtt
    17101 cgttctcctc cgaggcgtta cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agccggcgag ctcggccgcc gccgccgcga
    17221 cgcgcatgtc gagccggctt gcatccgccc actgcggctc gaagcgggcc acgtccgcct
    17281 cgcatgcagt acgccgcctc tcggcttcct cggactgcgc gcgcgcctcg tcgagcgcga
    17341 gcctggccgc ctcgaattgc tcggtgacgc gggccagcgc aacatctgcc cgctcgacat
    17401 cccggcgcaa gcggtcggca tcggcgagca gcgcccgcac cggctccac
//
LOCUS       DAORNH010000060        17424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2467_length_17424_cov_2.495596, whole genome shotgun sequence.
ACCESSION   DAORNH010000060 DAORNH010000000
VERSION     DAORNH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2467_length_17424_cov_2.495596"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1229)
                     /locus_tag="PK264_08310"
     CDS             complement(96..1229)
                     /locus_tag="PK264_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRD75927.1"
                     /translation="MAKSARAEVGAEEILAGIRRWVEIESQTADVAGVNRVMDAAEHE
                     WREAGAEVQRIAGRDGRGDHLLVTSPWGRGDGVLVLCHLDTVHPKGTLAADLPFRVEG
                     DKAFGPGIYDMKGGAYIALAAYREIVRAGRTTPLPIRVLIVSDEEVGSPTSRALIEAE
                     ADRARYVLVTEPARDGGKVVTGRKGVARYEMHARGRPAHSGSKHAEGRSAILEIARQI
                     VDIQAMTNYATGVTLNVGQIKGGTADNVVPEHCTAAIDMRVSRVEDAEMMDRRLKSLK
                     PYDPDVRLTITGGLNRPPYEKSNAVASLFEHARRLANELGFTLDDLSTGGGSDGNFTA
                     HKVATLDGLGVDGNGAHTLSEHLLISSLVPRMRLQQRLFETLS"
     gene            complement(1242..2192)
                     /locus_tag="PK264_08315"
     CDS             complement(1242..2192)
                     /locus_tag="PK264_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD75928.1"
                     /translation="MSDGPLGRAPSEPRTTAQKAVVAAILAMLLAGVSSSLLHLGVRV
                     VSEKVPTMVIVLLRATFTLMFTLPIVLRSADLPWRTNRPGLQLLRGAIGVLSMSTWYY
                     ALGQLPLADSGALSFTTGLFVTLGATIWFREVIGLRRIIALATGFLGVLIILRPGAGV
                     VSLAAFLAVFSSFLWAVSLLMAKELSKYDSTLTISFYQPLLIIPFALVGAVPVWVTPD
                     PWTLLILVGMGAIAAIGNYAYIFALKTADAAIVMPFDYVRLLYAAAWGYLFFSEVPGR
                     TTWIGAILIVGAAVFIAMRERQLAVPKVGAPGRDSSKAGS"
     gene            complement(2189..>2794)
                     /gene="purN"
                     /locus_tag="PK264_08320"
     CDS             complement(2189..>2794)
                     /gene="purN"
                     /locus_tag="PK264_08320"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRD75929.1"
                     /translation="IIILGKSADVVFEIVLVVSNKESAPGLAWARGEGVPTACVDHRA
                     YASREAFDGAVEAALLAARTELVAHAGFMRIQSDSFVQRWAGRQLNIHPSLLPSFKGL
                     KPHQQALEAGVRISGCTVHFVTAELDGGPIVAQAAVPVIEGDTAETLADRILVVEHRL
                     YPQALAHVATGRARLVGNRVQLDCDSDAPVDGALYSPTAPA"
     assembly_gap    2795..2869
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2871..4184)
                     /locus_tag="PK264_08325"
     CDS             complement(2871..4184)
                     /locus_tag="PK264_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD75930.1"
                     /translation="MTGQVLSPGVAALILFGLFFFLLFMRVPVAFALGLACVPILLIE
                     PRLGIMSVAQETFNAYNSFILLAVPFFLLTANLMNIGGTTDRLMKLSRTMVGHFPGGL
                     AQINVVLSIIFAGISGSSTADAASQSKIFIEAQRREGYDDSFSVAITAVSAVLAVIIP
                     PSILMIVWGGILNVSIGALFLAGILPGLLIGVVQMATVHAYAVRRGYPTYPKASWIDL
                     RCALWYAIPALMTPIIIIGGKGFFTATEAGCIAVIYAAILSLAVYREMDMRGLYQALA
                     DTGKLCGIALFCVGTASLFGWLLSYYQIPKAILSGVSSWQMGAIATGFFIAGVFLVVG
                     CFLDAIPAIIIVGTILEPLTKTVGMHPVHFAMIGIVSLAFGLVTPPYGLCLMIACSVA
                     GMRIRDAIRDTMIMLIPMFIVLVLVIVWPTLSLFLPTVVSPEFLK"
     gene            complement(4181..4699)
                     /locus_tag="PK264_08330"
     CDS             complement(4181..4699)
                     /locus_tag="PK264_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD75931.1"
                     /translation="MTETTEPTAARSLWRKATALYARLLDFFLVIGVAVLVLPVTLQV
                     VSRYVSWLPHFIWTEEMARFLFVWTIMIGAMVGVRESQHFEVDLWPRLSRRGEALVKI
                     IAKLGVLVFALAFVWGGYQFTAFAWNRISELAELPLWLIHVAWPVAGATWVLFLGEQF
                     WDELTIVLGRRA"
     gene            complement(4706..>5403)
                     /locus_tag="PK264_08335"
     CDS             complement(4706..>5403)
                     /locus_tag="PK264_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75932.1"
                     /translation="DLRGHGDSQWTSDGSYTMQGYLYDLAQLVHQLKLAPVPIVAHSL
                     GGMISTRYAATFPEQVTRMLLIEGLGLSPRMIAAAESKPATERLGKWVEKTRALAGRV
                     PRRYPTIEDAAARMREANRRLSEEQALHLTRNSLQQNEDGSWTWKFDPYLHVWPPYDI
                     PRAEVESLWRAIACPLLCIYGSESEASNPLTDGRAQHLARAEFTSISGAGHWVHHDRL
                     DAIVEHCDRFLSA"
     assembly_gap    5404..5413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5414..5603)
                     /locus_tag="PK264_08340"
     CDS             complement(<5414..5603)
                     /locus_tag="PK264_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD75933.1"
                     /translation="MPLQSSAVPGPISRTLVSQRLKLHYLDWGKPSAPPIVLVHGGRD
                     NCHTWDWLAPHLAQRFRVI"
     assembly_gap    5654..5797
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5914..7536
                     /locus_tag="PK264_08345"
     CDS             5914..7536
                     /locus_tag="PK264_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRD75934.1"
                     /translation="MIRLDSISKQNGHQILFIEASAALNKGDKIGLVGPNGAGKTTLF
                     RMITGEEQPDEGQVSVDRGITIGYFSQDVGEMAGRSAVAEVMEGAGPVAAVAAELKSL
                     EAAMADPDRAGDMDEIVEAYGVAQARFEEMDGYALDARAREVLAGLGFSQEMMEGDVA
                     TLSGGWKMRVALARILLMRPDAMLLDEPSNHLDIESLIWLETFLKGYEGMLFLTSHDR
                     AFMNRIVTRIIEIDGGTLTSYTGDYDFYEGQRKLAEAQQQAQFERQQAMLAKEIKFIE
                     RFKARASHAAQVQSRVKKLDKIEKVEPPRRRATVAFRFQPPPRSGEDVVSLKKVGKSY
                     GSRVIYDGLDFHVRRRERWCVMGVNGAGKSTLLKLVAGAATPDAGAVTVGASVKMGYF
                     AQHAMDLLDGEETVFRSLEQKFPQAGHGALRTLAGCFGFSGDDVEKRCRVLSGGEKAR
                     LVMAEMLFDPPNFLVLDEPTNHLDVATKAMLITALADYEGTMLFVSHDRHFLAALSNR
                     VLELTPEGVHTYGGGYTEYVARTGQEAPGLRG"
     gene            complement(7544..>8236)
                     /locus_tag="PK264_08350"
     CDS             complement(7544..>8236)
                     /locus_tag="PK264_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75935.1"
                     /translation="GFDAASPSDAEAAWQPWIGERMAARPRLTWLVVLEAHWQRSLRD
                     DEFAFGNMLREVFARVPDVEVRHRYFQNEASLIHWCRELLFIPEPVVLVITSHGEAEG
                     LAVGGEIIDTRKVIGTLEYAETIELLHFSCCLIAQDATGVLAKPPFPVSGYTTSVDWG
                     QSAMLEFIYLDMILGKKLSPAHAAAELPKLVAIAGDTVPEGSPYRAAGFRFFVPDGEA
                     PPAMPAATGAIA"
     assembly_gap    8237..8267
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8268..8717)
                     /locus_tag="PK264_08355"
     CDS             complement(<8268..8717)
                     /locus_tag="PK264_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75936.1"
                     /translation="MTTFQGRWWTTFGPMTLDQTGDRVTGTYLQGEQLCRIEGRVSGK
                     ELTFEYKEPDEAGRGIFRLERYGRFAGTYAIDGEGPRLPWEGSRGFDGLWKSSFGPLR
                     LIEQAGRVVGFYDVGGPAMIEGIVEGDKLTFRYVEQKARGHGWFKLDD"
     gene            complement(8714..>8963)
                     /locus_tag="PK264_08360"
     CDS             complement(8714..>8963)
                     /locus_tag="PK264_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75937.1"
                     /translation="ARSMLAAAGVFALSMVMRSADMSLCASWRYGTHALWHLLNAATL
                     FLLLRAVLDHEKCEQAGGDRPSGFPDRAGSSRIVGGSA"
     assembly_gap    8964..8975
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8976..9472)
                     /locus_tag="PK264_08365"
     CDS             complement(<8976..9472)
                     /locus_tag="PK264_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HRD75938.1"
                     /translation="MALLERIFDYCERGLDPAFWAEPVNALTNVAFIAVGGAAILSAS
                     ALPVAARVRWLPMALGALVVVIGIGSFLFHTFATRIAKLADVIPITIFMLVYLAVALR
                     LFLGFGWIAVAAILVVFAASMPLMERLPCRGPLSIVAHARGRCLNGTVGYLPAWIALI
                     AIGGVL"
     gene            9540..>10030
                     /locus_tag="PK264_08370"
     CDS             9540..>10030
                     /locus_tag="PK264_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75939.1"
                     /translation="MARATTAEPMTARDRQGILGLVAFAALVAGILYVVPPIAQRQDY
                     HQFADMRTCLGIANGYNVLSNLPFTVIGLLGMAALGRPGQLAVDRGLGYAYWVFFIGL
                     TLTGPGSIYYHLWPSNPTLVWDRLPIAVAFMGLYAAVIGERISELAGKLLLPFLVAAG
                     IWSV"
     assembly_gap    10031..10156
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10157..10337
                     /locus_tag="PK264_08375"
     CDS             <10157..10337
                     /locus_tag="PK264_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75940.1"
                     /translation="ITAIGFYVVAKALEELDRPIYALGTLVSGHTLKHLAAALGAFVV
                     YRMLVLRQPIAAPRA"
     assembly_gap    10489..10575
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10576..11815
                     /locus_tag="PK264_08380"
     CDS             <10576..11815
                     /locus_tag="PK264_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD75941.1"
                     /translation="AVEVTGIGRADSKVIKNQRKTFGTLRDLLRAKSTGIIEELPEKG
                     IVKWAKPVGVVAAITPSTNPQATPVNKAMMAVKGGNAIIIAPPPSAWAASEPAVALMR
                     AELKKIGLPEDLVQILPNPVTREATTRLMSLSDLVVATGSQANVKAAYSSGKPALGVG
                     LGNAPVIIDTTADLADAAKKIMLSKTFDHATSCSSENAIVIVDAVYDKAIAALAAEGG
                     YMASPDERQRIVERLWIDGKLNRAVIAQGPEALIEVFELGAKAKGCTFVMAEETGIGK
                     AYPLSGEKLSLVLTIYRARDFADAKRLVKAILDYQGRGHSCGIHTRDLAHARELAADT
                     DVVRVLVNQAHTFGNGGGYDNALPFTLTMGCGTWQGNAFSDNLSWRNFVNITHLVTTI
                     PEDRPSEEALFGAYWTKWGK"
     assembly_gap    12016..12045
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12274..14481)
                     /locus_tag="PK264_08385"
     CDS             complement(12274..14481)
                     /locus_tag="PK264_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD75942.1"
                     /translation="MLRTLLALVAALHVLATPARAAPDDIVIVIANEAYDGNVPKVEY
                     AKRDGAAMARFAREVLGLDSSRIAIVENATSGQLEDWFGRGGTIGPKLARLPIRKTST
                     IYAFYSGHGLPAKRSEAIGFEPFLLPVDTEPDAAGRRGLAVADMRAALLALQRQKAPE
                     GRVVLLLEACFSGSSSAGRIVPDTSFRISAKAIRDAEPRLVELSAADNEQEASWDRER
                     RHGIFTEAAMLGLYGAADANGDGHVTAAELAAFVGPRVERRVAQLFPNPRRYQTPMLT
                     TTEPDARLASLSARTPAWDAALKSEEGGFCAAYAASRDVTAIERFLATCTYCDARCRA
                     TLDDRARIVREEEARCAGETRVLDGLKARKAGKAEVEAFARTATCGRVRAAAEGLVAA
                     ASAPSPVASGAIWTGLRPMDPSPLLSPSPSAVVPPGTAPGSLVRSWQAHLYGVSGVAL
                     FPDRPWAVSASDDEHLKVWDLATGRELRTFAGHTGAVSGVAVSPNGKWLVSSSWDRTL
                     KLWDVATGRELRTLVGHKDWVLTVVRSPDGQWLASGSLDKTIKLWTAATGQELRTLTG
                     HSGAVSTIAVSTDGRWLASGSSDATIKIWDVATGQDVRTLAGHEQGVRAVAFSPDGRW
                     LISGSDDKTLRHWDLATGRELRTLEGHKGVVWAVSVSPDGQWIVSGGLDSTVQVWSAS
                     TGRHLRTLAGHSNYATSVSVSQDGRWILSAGCEEYNKKSYNCTRGDIKIWAMP"
     gene            14728..15525
                     /locus_tag="PK264_08390"
     CDS             14728..15525
                     /locus_tag="PK264_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD75943.1"
                     /translation="MKTFRDIAVERHGHVALIEIRRPPHNFFDIVLINEIADALEEID
                     KDIELRATVLASDGKSFCAGANFGDGSQLSKEGMRPGESARDTSGSLYMQAVRLFRAK
                     KPIIGAIHGPAIGGGLGLALVPDFRVTCPEARFATNFTRLGFHPGFGLTTTLKELVGP
                     TKAALMFYTSRRVGGEEAVAMGLADVLVPLEQVRATALALAKEISENAPLAVMATRAT
                     MRQGLADRVRAATEHELNQQTELRASEDFKEGVRAVHERRTPVFKGR"
     gene            15530..16591
                     /locus_tag="PK264_08395"
     CDS             15530..16591
                     /locus_tag="PK264_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HRD75944.1"
                     /translation="MAAGTDRPDRGGAQFASDNNAGMCPEALAALIEANGAGHALGYG
                     GDPWTERACNAIRALLEAPEAEVFLVYNGTAANALSLAQLCRSYNAVIAHAFSHIEWD
                     EAGAVGFFSGGASLITADTAGGKLTPGEIERLATKFGRGFHHVKPAAVSITQATEVGT
                     VHTLAELRVLTETAHRLGLKVHLDGARFANAVASLGCSAADMSWRAGVDVLSLGGVKN
                     GLGIGEAVVFFNPADAHEFEWRMKQAGQINSKMRLMAAPWVGLIETGAWRRHAEHANA
                     MARRLGSGLAALSGFRLLHPVEANAVFADIPVATQERLRAKGWLFYTFLGATGCRLMC
                     AWDTPAATVDAFLADAAAS"
     gene            complement(16657..16875)
                     /locus_tag="PK264_08400"
     CDS             complement(16657..16875)
                     /locus_tag="PK264_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75945.1"
                     /translation="MLKSIIIASAAALTIAGSTISSADAGYRRHHFYGYGHVHKPYYG
                     YSHGYSAPTCVWKKVWFHGGYHWKRFCW"
     assembly_gap    17110..17119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17120..>17424
                     /locus_tag="PK264_08405"
     CDS             <17120..>17424
                     /locus_tag="PK264_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD75946.1"
                     /translation="RGRALLAEDERYFLRQSVSTPCLNAIAKAEGIWIEDTAGRRYMD
                     FHGNNVHHIGYGHPRLKAAIVAEMDRLPFAPRRYACDTAVALARKIATITPGGLSKM"
BASE COUNT         3027 a         5702 c         5603 g         2567 t
ORIGIN      
        1 atgcgctcct cgagttcgcg ataggcctgc tcggtcaacg attccgcccg ctctccgggg
       61 ctgcgctcga cggttgcctc gctcattttg gtgcctcaag agagagtttc gaagaggcgc
      121 tgctgaagcc tcatccgcgg caccagcgac gagatcagga ggtgctcgga aagcgtgtgg
      181 gcgccattgc cgtcgacgcc gaggccatcg agcgtcgcga ccttgtgggc cgtgaaattt
      241 ccgtcgctgc cgccgcccgt cgagagatcg tcgagcgtga agcccagctc gttcgcgagg
      301 cgccgcgcat gctcgaacag ggaggccacc gcattgctct tctcgtaggg cggccggttg
      361 agcccgcccg tgatcgtgag acgcacatcg ggatcataag gcttcagcga cttcagacgg
      421 cgatccatca tctcggcatc ctcgacgcgc gagacgcgca tgtcgatcgc cgccgtgcag
      481 tgctccggca ccacgttgtc ggccgtgccg cccttgatct ggccgacgtt aagggtgacg
      541 ccggtcgcat aattggtcat ggcctggatg tcgacgatct gacgcgcgat ctcgaggatg
      601 gcactgcgcc cctcggcgtg tttgctgccc gaatgcgcgg gccggccgcg cgcgtgcatc
      661 tcgtagcgcg cgacgccttt gcggcccgtc acgaccttgc cgccgtcgcg cgccggttcg
      721 gtaacgagca cgtagcgcgc gcggtcggcc tctgcttcga tcagcgcccg cgacgtcggg
      781 ctgccgacct cctcatccga gacgatcagg acgcggatcg gcagcggcgt cgtgcgcccg
      841 gcgcggacga tctcccggta ggccgcgagt gcgatgtagg cgccgccctt catgtcgtag
      901 atgccggggc cgaaggcctt gtcgccctcg acgcggaacg gcaggtcggc ggcgagcgtg
      961 cccttgggat gcacggtgtc gagatggcag aggacgagca cgccgtcgcc tcgtccccac
     1021 ggcgacgtca cgaggaggtg gtcgccgcgc ccgtcccggc ccgcgatgcg ctgcacctcg
     1081 gcgccggcct cgcgccattc gtgctccgcc gcgtccatga cgcggttgac gcctgcgaca
     1141 tccgccgtct ggctttcgat ctcgacccag cgccggatac cggcgaggat ctcctcggca
     1201 ccgacctctg ctctcgctga cttagccatc ctgatcctcc cttacgatcc cgctttgctg
     1261 ctgtcccggc ccggcgcccc gactttcggg accgcgagtt gccgctcccg catggcgatg
     1321 aagaccgcgg cgccgacaat gagtatggcc ccgatccagg tggtgcggcc aggaacctca
     1381 ctgaaaaaga gataacccca ggcggccgca tagaggagcc gcacatagtc gaagggcatg
     1441 acaatcgcgg catcggccgt cttcagcgcg aagatgtagg catagttgcc gatcgcggcg
     1501 atcgccccca tgccgacgag gatgaggagc gtccacgggt cgggcgtcac ccagaccggg
     1561 accgcgccga cgagcgcgaa ggggatgatc agcagcggct gatagaacga gatcgtcagc
     1621 gtcgaatcgt acttcgagag ttccttcgcc atcagcagcg agaccgccca caggaagctc
     1681 gagaacaccg cgaggaaggc cgcaagcgag acgacgcctg caccaggtcg caggatgatc
     1741 agcacgccga gaaagcccgt cgccagcgcg atgatccgcc ggagcccgat cacctcgcgg
     1801 aaccagatcg tcgcgcccag cgtgacgaac agccccgtcg tgaagctcag cgcacccgaa
     1861 tcggcgagcg gcaactggcc aagcgcatag taccacgtgg acatggagag cacgccgatc
     1921 gcgccgcgca gcagttggag cccgggccgg ttggtccgcc acggcagatc ggcggaccgg
     1981 agcacgatcg gcagcgtaaa catcagcgtg aaggtggcgc gcaggagcac gatgaccatc
     2041 gtcggcacct tctcgctgac cacccgcacg ccgagatgca gcagcgacga cgagacgccg
     2101 gcaagcagca ttgcgagaat tgcggcaacg accgcctttt gggctgtagt gcgcggctcg
     2161 gagggcgcac gccccagcgg accgtcactc atgccggtgc tgtgggcgag tacagtgcgc
     2221 catccactgg cgcatcggaa tcgcagtcga gctgcacacg gttgccgaca agtcgcgcgc
     2281 ggcctgtcgc aacgtgcgcc agcgcctgcg gatagagccg atgctcgaca accaggatgc
     2341 ggtccgccag cgtctcggcc gtatcgcctt cgatcacagg gacagcggcc tgcgcgacga
     2401 tcggaccacc atcgagctcg gccgtcacga aatgcacggt gcagccggaa atgcgcacgc
     2461 cggcttcgag cgcctgctgg tgcggtttca gacccttgaa ggaggggagc agcgaagggt
     2521 gaatgttgag ctgccggccc gcccaacgct gcacgaaaga gtcggactgg atgcgcatga
     2581 agccggcgtg cgccacgagt tcggtcctcg ccgcgagcag cgccgcctcg acggccccgt
     2641 cgaaggcctc gcgcgaggcg tacgccctgt gatcgacgca ggccgtcggc acgccttccc
     2701 cgcgcgccca ggcgagaccc ggcgcgctct ccttgttcga gacgacgagg acgatctcga
     2761 agacgacatc ggcggacttt ccgagtatga tgatnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tcacttcaga
     2881 aactccggcg agacaacggt cggcaggaac agcgagagcg tcggccacac gatgacgagc
     2941 acaaggacga tgaacatcgg gatcagcatg atcatggtat cgcgtatggc atcgcgtatg
     3001 cgcattccgg cgacggagca ggcgatcatc agacagagac catagggcgg cgtcacgaga
     3061 ccgaaggcca gcgagacgat gccgatcatg gcgaagtgca cggggtgcat gccgacggtc
     3121 ttggtgagcg gctcgagaat ggtgccgacg atgatgatcg ccgggatggc gtcgaggaag
     3181 cagccgacga cgaggaacac gccggcaatg aagaagccgg tcgcaatcgc acccatctgc
     3241 caggatgaaa cccccgagag gatcgctttc ggaatttggt agtaggagag gagccagccg
     3301 aagaggcttg ccgtgccgac gcagaagagc gcgatgccgc agagctttcc cgtgtctgca
     3361 agcgcctgat agagccccct catgtccatc tcgcgataga cggcgagcga gaggatcgcg
     3421 gcatagatga cggcaatgca gccggcctcc gtcgccgtga agaagccctt gccgccgatg
     3481 atgatgatcg gcgtcatgag cgccgggatc gcataccaga gcgcgcatct gagatcgatc
     3541 catgaggcct tggggtaggt cgggtagccc cgtctcacgg cataggcgtg cacggtcgcc
     3601 atctgcacga cgccgatcag caggcccggt aggatgccgg cgaggaacag cgcgccgatc
     3661 gagacgttca ggatgccgcc ccacacgatc atcaggatcg acggcgggat gatcacggcg
     3721 agcacggccg agaccgccgt gatggcgacg gagaagctgt cgtcgtagcc ctcgcgacgc
     3781 tgcgcctcga tgaagatctt cgactggctc gcagcatccg ccgtggacga ccccgatatg
     3841 ccggcgaaga tgatcgagag caccacgttg atctgcgcga gcccgcccgg gaagtgcccg
     3901 accatggtgc gcgagagctt catcaggcgg tcggtggtgc cgccgatgtt catgaggttc
     3961 gccgtcagca gaaagaacgg caccgcgagc aggatgaacg agttgtaggc gttgaaggtc
     4021 tcctgggcga ccgacatgat gccgagacgc ggctcgatca gcaggatcgg cacgcacgcg
     4081 agaccgagcg cgaaggcgac gggcacacgc atgaacagca ggaagaagaa gagcccgaag
     4141 aggatgagcg ccgcaactcc cggcgagagc acctggccgg tcatgcgcgc ctcccgagga
     4201 cgatcgtgag ctcgtcccag aactgctcgc cgaggaacag aacccacgtc gcgcccgcga
     4261 ccggccaggc cacatggatg agccagagcg gcagctcggc cagctccgag attcggttcc
     4321 aggcgaaggc cgtgaactgg tagccgcccc acacgaaggc gagcgcgaaa acgaggacgc
     4381 cgagcttggc gatgatcttc acgagggcct cgccgcgtcg cgacagcctc ggccagaggt
     4441 cgacctcgaa gtgctggctc tcgcgcacac cgaccatggc gccgatcatg atcgtccaca
     4501 cgaacaggaa gcgcgccatc tcctcggtcc agataaagtg cggcagccac gagacgtaac
     4561 gcgagacgac ctgcagcgtg accgggagca cgagcaccgc gaccccgatg accagaaaga
     4621 agtccagcaa gcgcgcatag agtgccgtgg ccttccgcca gagcgagcgt gcggcagtcg
     4681 gttcagtggt ctcggtcatg gggcctcaag ctgagagaaa gcggtcgcag tgctcgacaa
     4741 tggcatcgag ccggtcgtga tgcacccaat gacccgcacc ggagatcgaa gtgaactcgg
     4801 cccgggcgag atgctgggcg cgaccatcgg tcagcgggtt cgaggcctcg ctttccgacc
     4861 cgtaaatgca gagcagcggg cacgcgatgg cacgccagag tgattcgacc tcggcgcgcg
     4921 ggatgtcata gggcggccag acgtggaggt agggatcgaa cttccatgtc caggagccgt
     4981 cctcgttttg ctgcaggctg ttccgcgtca gatgcagcgc ctgctcctca cttagccgac
     5041 ggttcgcctc ccgcatgcgc gcggcggcgt cctcgatcgt cggatagcgc cgcggcacgc
     5101 gcccggcgag tgcccgtgtc ttctcgaccc attttccgag ccgctcggtc gcgggcttgc
     5161 tctcggcggc ggcgatcatg cgcggcgaga ggccgaggcc ctcgatcagc agcatgcgcg
     5221 tcacctgctc ggggaaggtc gcggcatagc gcgtcgagat catgccgccg agcgagtggg
     5281 cgacgatcgg caccggcgcg agcttcaact ggtggacgag ctgggcgaga tcgtagaggt
     5341 agccctgcat cgtatagctt ccatcgctgg tccactggct gtcgccgtgg ccccgcaggt
     5401 cagnnnnnnn nnncgatgac gcggaagcgc tgcgccagat gcggggcgag ccagtcccac
     5461 gtatggcagt tgtcgcgccc gccgtgcacg agcacgatcg gcggcgcgga gggcttcccc
     5521 caatcgaggt agtgaagctt caggcgctgc gagacgagcg tccgcgagat cgggccgggg
     5581 acggcggagg actggagtgg catgggggcg ggcgttctcg gttggctgga gccgcatagc
     5641 tcgcacagct ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa cacacatcga cacttccgcc
     5821 cgcagatatc ggctcgccgt cgcgagatca gacccctttc gcttcccgcc tacctcgcct
     5881 atgagcgccg ggtcgtctcc caagggcccc cgcatgatcc gtctcgacag catcagcaag
     5941 cagaacggcc accagatcct cttcatcgag gcctcggccg cgctcaacaa gggcgacaag
     6001 atcggcctcg tcggcccgaa cggtgccggc aagacgacgc tcttccgcat gatcaccggc
     6061 gaggagcagc cggacgaagg ccaggtgtct gtcgatcgcg gcatcacgat cggctatttc
     6121 agccaggacg tcggcgaaat ggcgggccgc agcgccgtcg ccgaggtgat ggaaggcgca
     6181 ggcccggtcg ccgccgtcgc ggctgaactc aaatcgctcg aggccgccat ggccgatccc
     6241 gatcgcgccg gcgacatgga cgagatcgtc gaagcatacg gcgtcgcgca ggctcgcttc
     6301 gaggagatgg acggctacgc actcgatgcc cgcgcccgcg aggtgctcgc cggcctcggc
     6361 ttctcgcagg agatgatgga gggcgatgtt gccacgctct cgggcggctg gaagatgcgc
     6421 gtcgcgcttg cccgcattct cttgatgcgc cccgacgcca tgctgctcga cgagccctcg
     6481 aaccacctcg acatcgaaag cctcatctgg ctcgagacct ttctcaaggg ctacgagggc
     6541 atgctgttcc tcacctcgca cgaccgcgcc ttcatgaacc gtatcgtcac gcgcatcatc
     6601 gagatcgatg gcggcacgct cacgagctac acgggcgact acgactttta cgaggggcag
     6661 agaaagctcg ccgaggcgca acagcaggcg cagttcgagc gccagcaggc gatgctggcg
     6721 aaggagatca agttcatcga gcgtttcaag gcgcgcgcct cgcatgcagc ccaggtgcag
     6781 agccgcgtga agaagctcga caagatcgag aaggtggagc cgccacgccg gcgcgcgacg
     6841 gtcgccttcc gctttcagcc gccgccgcgc tcgggcgagg acgtggtgag cctgaagaaa
     6901 gtcggcaaga gctacggcag ccgcgtcatc tacgacggcc tcgatttcca cgtgcggcgc
     6961 cgcgagcgct ggtgcgtgat gggcgtcaac ggcgccggaa agtcgacgct gctgaagctc
     7021 gtcgccggcg cggcgacacc ggacgcaggc gccgtgacgg tcggcgcgag cgtgaagatg
     7081 ggctacttcg cgcagcacgc gatggacctc ctcgacggcg aggagaccgt gttccgctcg
     7141 ctcgagcaga aatttccgca ggccgggcat ggcgccctgc gcacgctcgc cggctgcttc
     7201 ggcttctcgg gcgatgatgt cgagaagcgc tgccgcgtgc tctcgggcgg cgagaaggcg
     7261 cgcctcgtca tggccgagat gctgttcgat ccgccgaact tcctcgtgct cgacgagccg
     7321 accaaccacc tcgacgtcgc gaccaaggcg atgctcatca cggcgcttgc cgactacgag
     7381 gggacgatgc tgttcgtctc ccacgatcgc cacttcctcg cagctctctc gaaccgggtg
     7441 ctcgagctca cacccgaggg cgtccacacc tatggcggcg gctatacaga gtatgtcgcg
     7501 cgcacgggcc aggaagctcc gggtctcagg ggatgagagc cgatcaggcg atggcgcctg
     7561 ttgccgctgg catcgcgggc ggcgcttcac cgtcgggaac gaagaagcgg aagcccgcgg
     7621 cgcggtaggg cgagccctcg ggcaccgtgt cgccggcgat ggcgacgagc ttcggcagct
     7681 cggccgccgc gtgtgcgggt gacagcttct tcccgagaat catgtcgaga tagatgaact
     7741 cgagcatggc gctctgcccc cagtcgacgc tggtcgtgta gcccgagacc gggaatggcg
     7801 gcttcgccaa cacgccggtc gcgtcctgcg cgatgaggca gcaggagaag tgcaggagct
     7861 cgatcgtctc ggcatactcg agcgtgccga tcaccttgcg cgtatcgatg atctcgccgc
     7921 cgaccgcgag accctccgcc tcgccgtggc tggtgatcac gagcacgacg ggctccggaa
     7981 tgaaaagcag ctcgcggcac cagtggatga ggctcgcctc gttctggaag tagcgatggc
     8041 gcacctcgac gtcgggtacc cgcgcgaaca cctcgcgcag catgttgccg aaggcgaact
     8101 cgtcgtcgcg gagcgagcgc tgccagtggg cctccagcac cacgagccag gtgaggcggg
     8161 gccgcgcggc catgcgctcg ccgatccagg gctgccaggc cgcctccgcg tccgatgggc
     8221 tcgccgcgtc gaaaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc gtcgagcttg
     8281 aaccagccgt gcccgcgcgc cttctgttcg acgtagcgga acgtgagctt gtcgccctcg
     8341 acgatccctt cgatcatcgc cggaccgccg acatcgtaga agccgacgac gcggcccgcc
     8401 tgctcgatga gacggagcgg gccgaaggaa ctcttccaga ggccgtcgaa gccgcgcgag
     8461 ccctcccagg ggaggcgcgg gccttcgcca tcaatcgcat aggtgccggc gaagcggcca
     8521 taccgctcga gcctgaagat gccgcggccc gcctcgtccg gctccttgta ctcgaaggtg
     8581 agctctttgc cggagacccg cccctcgatc cggcagagct gctcgccctg gagatacgtg
     8641 ccggtgacgc ggtcgccggt ctggtcgagc gtcatcggac cgaacgtggt ccaccagcgc
     8701 ccctgaaacg ttgtcatgcc gagcccccga cgatccgcga cgaacccgcc ctatctggga
     8761 agcccgaagg cctgtcgcca ccggcctgct cacatttttc gtgatccaat acggccctca
     8821 ggaggagaaa cagagttgcg gcgttgagaa gatgccagag cgcgtgcgtg ccgtagcgcc
     8881 aactcgcgca tagcgacatg tctgccgatc gcatgaccat cgagagggcg aacacacctg
     8941 ccgccgccag catcgatcgc gcgnnnnnnn nnnnnagcac gccgccaatg gcaatgagcg
     9001 caatccaggc tgggagatag ccgacggtgc cgttgaggca gcgcccgcgg gcatgcgcga
     9061 cgatggacag cggcccgcga caagggagcc gctccatgag tggcatggat gcggcgaaca
     9121 cgacgaggat tgcggcgacc gcgatccatc cgaaaccgag gaacaggcgc agggccacgg
     9181 cgaggtagac gagcatgaag atcgtgatcg gaatgacgtc ggcgagcttg gcgatgcgcg
     9241 tcgcgaaggt atggaagagg aagctgccga tgccgatcac gacgaccagc gcgccgagcg
     9301 ccatgggcag ccagcggaca cgcgccgcga ccggcagagc gctggccgag aggatcgccg
     9361 cgccaccgac ggcgatgaac gcgacattgg tcagcgcatt cacgggttcg gcccagaacg
     9421 ccggatcgag gccgcgctcg cagtagtcga atattcgctc gaggagtgcc atggtcggct
     9481 cggatcgggg gccgcggttc gattcggacg atgcggcagg gaatgagggg tggcaaggca
     9541 tggcgcgagc aacgaccgca gagccgatga cggcccgtga ccggcaaggc atcctgggcc
     9601 tcgttgcctt tgccgctctg gtcgccggta ttctgtatgt cgtcccgccg atcgcgcaac
     9661 gccaggacta tcaccagttc gccgacatgc gcacctgcct cggcatcgcc aacggctaca
     9721 acgtgctctc gaacctgccg ttcaccgtga tcggcctcct cggcatggca gcactcggcc
     9781 gccccggcca gctcgccgtc gatcgcggcc tcggctatgc gtactgggtg ttcttcatcg
     9841 ggctcaccct caccggtccc ggctcgatct actaccacct ctggccgagc aacccgacgc
     9901 tcgtatggga ccggctgccg atcgcggtcg ctttcatggg gctctatgcg gcggtgatcg
     9961 gcgagcgcat ctcggagctc gccggcaaac tgctgctccc tttcctcgtc gccgccggca
    10021 tctggagcgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnncatc acggcgatcg gcttctatgt cgtcgccaag gcgctcgagg
    10201 agctggaccg gcccatctac gcgctcggca cccttgtcag cggccatacc ctcaagcacc
    10261 tcgccgcggc ccttggcgcc ttcgtggttt atcgtatgct ggtgctgaga cagccgatcg
    10321 ccgcaccgcg cgcctgaccg cgggccggcc tggaggacgc catgcaggat acgccgtcgc
    10381 agatgaaggg ccggaccgcg agcagcaacg gcgccaatga accggccgcc gaccccgttc
    10441 cggcgctgat cacgcgcgct cgcgccgcca tggcgacgtt tgccggcgnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnncgccg tcgaggtgac agggatcggc cgcgccgaca gcaaggtgat
    10621 caagaaccag aggaagacat tcggcacgct gcgcgatctg ctccgcgcaa agtcgaccgg
    10681 catcatcgag gagctccccg agaagggcat cgtcaagtgg gcgaagccgg tgggcgtcgt
    10741 cgccgcgatc acgccttcga ccaatccgca ggcgacgccg gtcaacaagg ccatgatggc
    10801 cgtgaagggg ggcaacgcca tcatcatcgc gccgccgccc tcggcctggg ccgcgagcga
    10861 gccggccgtc gccctcatgc gcgccgagct caagaagatc ggcctacccg aggacctcgt
    10921 gcagatcctg cccaatccgg tgacgcgcga ggcgaccacc cggctcatgt cgctttcgga
    10981 tcttgtcgtc gcgaccggca gccaggcgaa cgtgaaggcc gcctactcgt cggggaagcc
    11041 cgccctcggc gtcggcctcg gcaacgcgcc ggtgatcatc gacacgacgg ccgatctcgc
    11101 cgacgcggcg aagaagatca tgctctcgaa gaccttcgac cacgcgacga gctgctcctc
    11161 cgagaacgcc atcgtcatcg tcgacgcggt ctacgacaag gccatcgccg cgctcgccgc
    11221 cgagggcggt tacatggcga gccccgacga gcgccaacgc atcgtcgagc ggctctggat
    11281 cgacggcaag ctcaaccgcg ccgtgatcgc ccaggggccc gaggcactga tcgaggtgtt
    11341 cgagctcggc gcgaaagcca aaggctgcac cttcgtgatg gccgaggaga cgggcatcgg
    11401 caaggcctac ccgctctccg gcgagaagct ctcgctcgtc ctcaccatct atcgcgcgcg
    11461 cgacttcgcg gatgccaagc gcctcgtcaa ggcgatcctc gactaccagg gccgcggcca
    11521 ctcctgcggc atccacaccc gcgacctggc gcacgcccgc gagcttgcgg cggacaccga
    11581 cgtcgtacgc gtgcttgtca atcaggcgca caccttcggc aacggcggcg gctacgacaa
    11641 cgcgctcccc ttcacgctga cgatgggctg cggcacctgg caaggcaacg ccttctccga
    11701 caacctctcc tggcgcaact tcgtcaacat cacccacctg gtgaccacca tccccgaaga
    11761 ccgcccgagc gaagaagcct tgttcggcgc ctattggacg aagtggggga agtagcgggc
    11821 cccgcgcatg gctgtcatcc cggacgccgg agcggccctt ctgccgcttg tgcgtccggg
    11881 acccagcgca atgagcggcg cagccaccgt atctctttca cccgcaatac cgggcgctgc
    11941 gcgcgctcct tacgctgggt cccggctcgg cgcgcccgca atagcgtgcg cttggccggg
    12001 gtgacagtga gggggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaatt cggcggacgc
    12061 gcagcggccg gccgaaggtc cgggatcttt ccctgccgat gccgcgcgac acctcgcacc
    12121 acgcgccgct gccgacgagc acaccatcta cgggaaagac cccggacgag cgctgccgcg
    12181 ctctcaaatt ggccggcgca tgcaccgggc ggtgtcgcat ggggccgcct gcgcggccct
    12241 gacgaacgtg ggcgaacggt cgcgcccgac ggcctacggc attgcccaaa tcttgatgtc
    12301 gccgcgcgtg cagttgtacg attttttgtt gtattcttcg cacccggcac tcagtatcca
    12361 tcgcccatcc tgcgaaacag aaacagaggt ggcgtagttc gaatgtcccg cgagggtgcg
    12421 aaggtgtctt cccgtcgagg cactccagac ttgcactgta ctatccaagc cacccgagac
    12481 tatccactga ccatccggag acacgctaac cgcccacacc acgcccttat gcccctctag
    12541 tgtgcgcagt tcacgacccg tcgccaagtc ccaatgcctt agcgtcttat cgtcgcttcc
    12601 tgaaatcaac catcgaccgt ctggagaaaa tgcaaccgct ctcacccctt gctcgtgccc
    12661 ggcgagagtt cgtacgtctt gcccggtggc cacgtcccag atctttattg ttgcatcaga
    12721 actccccgac gccagccaac gcccgtccgt cgacaccgca atagtagaca ctgccccgga
    12781 atgcccagtc agggtgcgca gctcctgacc agtagccgcg gtccagagct taattgtttt
    12841 gtccagacta cccgaggcca gccactggcc atccggcgac cgtacgacag tcagcaccca
    12901 atccttgtgt ccgacgagcg tgcgcaactc tcgcccagtc gcaacatccc agagcttcaa
    12961 tgttcgatcc cagctcgatg acacaagcca cttcccgtta ggtgacacgg caacgccgga
    13021 cacggcgccg gtgtgacctg caaaagtgcg cagctctcga ccggtcgcca aatcccacac
    13081 cttcaaatgt tcgtcatcac tcgccgacac cgcccaaggc ctgtcgggaa acaatgccac
    13141 gcccgacacc ccgtataggt gcgcctgcca cgacctgacc agcgatcccg gggccgttcc
    13201 tggcggcacg acggcggacg gcgaaggaga cagtaatggt gaaggatcca tcggtctgag
    13261 accagtccag atcgcaccag acgccaccgg cgatggagcc gacgcggccg ccaccagccc
    13321 ctccgccgcc gccctcactc tcccgcacgt cgcggtcctg gcgaacgcct ccacctccgc
    13381 cttgccggcc ttgcgcgcct tgaggccgtc gagcacgcgg gtctcgccgg cgcagcgcgc
    13441 ctcctcctcg cggacgattc gcgcacgatc atcgagcgtc gcgcggcagc gggcgtcgca
    13501 gtaggtgcac gtcgcgagga agcgctcgat cgcggtcaca tcgcgcgagg ccgcataggc
    13561 tgcgcagaag ccaccctcct cgctcttcaa ggcggcgtcc caggcaggcg tgcgggccga
    13621 aaggctcgca agacgcgcgt ccggctcggt cgtcgtcaac atgggcgtct ggtaacggcg
    13681 cgggttcggg aagagctgcg ccacccgccg ctcgacccgc ggaccgacga aagcggcaag
    13741 ctccgccgcc gtaacatggc cgtcgccgtt cgcatccgcc gcgccataga ggccgagcat
    13801 ggcggcctcc gtgaagatgc cgtggcggcg ctcgcgatcc cagctcgctt cctgctcgtt
    13861 gtcggcagcg gaaagctcga cgaggcgtgg ctcggcatcg cggatcgcct tcgccgagat
    13921 gcggaagctc gtgtccggca cgatgcgccc ggcactcgac gagcccgaga agcaggcttc
    13981 gaggagaagg acgacgcgcc cttccggcgc cttctgccgc tgcagcgcga gaagagccgc
    14041 ccgcatgtcg gcgacggcga gaccgcgacg gcccgcggca tcgggctcgg tatcgaccgg
    14101 tagcaggaac ggctcgaagc cgatcgcctc cgagcgcttg gcgggcaggc cgtgccccga
    14161 atagaacgcg tagatcgtgg acgtcttccg gatggggagc cgcgcgagct tcggaccgat
    14221 cgtgccgcct cggccgaacc aatcctcgag ctggcccgac gtggcattct cgacgattgc
    14281 gatgcgcgag ctgtcgagtc cgagcacctc gcgcgcgaag cgggccatcg ccgccccatc
    14341 gcgcttcgca tactcgacct tcggcacgtt gccgtcatag gcctcgttgg cgatgacgat
    14401 gacgatgtcg tccggcgccg cgcgcgccgg agttgccagc acgtgaagcg cggcgacgag
    14461 cgccagcaga gtgcgcagca taagccctcc gtcggaaaac agggcgaatg tacaccaagc
    14521 cgcgcgggaa gcgaaagcgt cgggcgcggc gatcggcaat cgcagacgag tcgcgcggga
    14581 ctgcctgcgg ggttccgacg atcgcggacc gtcgttgcgc tcagcgcgag cctgctgaag
    14641 ctcgtccccc gcgcaaaagc gcttcacgtc attcgacggg tggctatagt ccagctcgag
    14701 catcggacaa tccccgggag acgccccatg aagacctttc gcgacatcgc cgtcgagcgg
    14761 catggccacg tggcgctgat cgagatccgc cgtccgccgc acaacttctt cgacatcgtg
    14821 ctgatcaacg agatcgcgga cgcgctcgag gagatcgaca aggacatcga gctgcgcgcg
    14881 accgtgctcg cctcggacgg caagtcgttc tgcgcgggcg ccaacttcgg cgacggctcg
    14941 cagctttcca aggagggcat gcggccgggc gagagtgcgc gcgatacctc gggcagcctc
    15001 tacatgcagg cggtgcggct gttccgcgcc aagaagccga tcatcggcgc cattcatggc
    15061 cccgcgatcg gcggcgggct cggattggca ctcgttccag atttccgcgt gacgtgcccg
    15121 gaagcgcgct ttgcgacgaa cttcacgcgg ctcggcttcc atccgggctt cgggcttacg
    15181 accacgctca aggagcttgt cggcccgacg aaagcggcgc tcatgttcta cacgagccgg
    15241 cgcgtcggcg gcgaggaggc ggtcgccatg gggctcgctg acgtgctcgt accgctcgag
    15301 caggtgcgcg cgacggcgct ggcactcgcc aaggaaatct ccgagaatgc gccgctcgcc
    15361 gtgatggcga cacgggcgac gatgcggcag ggccttgctg atcgtgtgcg cgcggcgacc
    15421 gagcacgagc tcaaccagca gacggagctg cgggcgagcg aggacttcaa ggaaggtgtg
    15481 cgcgcggtgc acgagcgccg cacgcccgtc ttcaaggggc gctgaagcaa tggccgcggg
    15541 gacggatcga cccgaccgag gcggcgccca gttcgcctcg gacaacaacg ccggcatgtg
    15601 cccggaggcg ctcgcggcgc tgatcgaggc gaacggcgcc ggccatgcac tcggctatgg
    15661 cggcgatccc tggaccgagc gcgcctgcaa cgcgatccgc gccttgctcg aggcgcccga
    15721 ggccgaggtc ttcctcgtct acaacggcac ggcggcgaac gcgttgtcgc tcgcccagct
    15781 ctgccgctcg tacaacgccg tgattgcgca tgctttctcc catatcgagt gggacgaggc
    15841 gggcgcggtc ggcttcttct cgggcggcgc gagcctgata acggccgaca cggcgggcgg
    15901 caagctgacg cctggcgaga tcgagcgcct cgccaccaag ttcggtcgcg gcttccacca
    15961 cgtgaagccg gccgccgtct caattacaca ggcgacggaa gtcggcaccg ttcacacgct
    16021 cgcggagctt cgcgtgctca ccgagacggc ccatcgcctc ggcctcaagg tacacctgga
    16081 cggcgcgcgc ttcgccaacg ccgttgcgag cctcggctgc tcggcggccg acatgagctg
    16141 gcgcgccggc gtcgatgtgc tctccctcgg cggcgtcaag aacggactcg ggatcggcga
    16201 ggcggtcgtg ttcttcaatc cggcagatgc gcacgagttc gagtggcgca tgaagcaggc
    16261 gggccagatc aactcgaaga tgcggctgat ggcggcgccc tgggtcggac tcatcgagac
    16321 gggcgcgtgg cgacgccatg ccgagcacgc caacgcgatg gcacggcggc tcggctcggg
    16381 gctcgcagca ctttccggct tcaggctcct ccatccggtc gaggccaatg cggtgttcgc
    16441 cgacattccg gtcgcgacgc aggagcggct ccgcgccaag ggctggctct tctacacctt
    16501 cctcggcgcg accggctgcc gactgatgtg cgcctgggat acgccggctg cgaccgtcga
    16561 tgctttcctc gctgatgcgg cggcaagttg aaagccgcct ccggaaccgc ggggtgggct
    16621 ccggaggctg cctctgcggg ccatcaggcg tggcgattac cagcagaagc gcttccagtg
    16681 gtagccgccg tggaaccaca ccttcttcca cacgcaggtc ggcgcgctgt agccgtgcga
    16741 gtagccgtag tagggcttat ggacatggcc gtagccgtag aagtggtggc ggcgataacc
    16801 ggcatcggca ctcgagatgg tcgagccggc gatcgtcaga gcggcggcgg aagcgatgat
    16861 gatggacttg agcatgggat cagttcctct gcaggttctg gcggagcgac catcgcgccg
    16921 ccgatgagtg gagaatgccg aaagtggctc ccgggcgcgg tgctctccag cacagggctg
    16981 aaagcaccag caggcggccg gtgaaacgga ggacgatgcc atggcggcga agcgcaccga
    17041 ggtctcccag tccgagggcg acacgaaccc ctgagcccgg cgagcgccgt ggagatgtcc
    17101 gatcgcacan nnnnnnnnnc ccgtggtcgc gctctcctcg ccgaggatga gcgctatttc
    17161 ctccgccagt cggtctcgac gccctgcctc aacgcgatcg ccaaggccga gggcatctgg
    17221 atcgaggaca ccgccggccg ccgctacatg gacttccacg gcaacaacgt gcaccacatc
    17281 ggctacggcc atccgcggct caaggcggca atcgtcgccg agatggatcg cctcccgttc
    17341 gcgccgcgcc gctatgcctg cgatacggca gtcgcgcttg cacggaagat cgcgacgatc
    17401 acgcccggcg ggctctcgaa gatg
//
LOCUS       DAORNH010000061        17403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2470_length_17403_cov_2.722101, whole genome shotgun sequence.
ACCESSION   DAORNH010000061 DAORNH010000000
VERSION     DAORNH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17403
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_17403_cov_2.722101"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PK264_08410"
     CDS             complement(<1..704)
                     /locus_tag="PK264_08410"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD75947.1"
                     /translation="MTAASEVSPFSVPAFRTFWAARVIATIAYQALSIAIGWKLYELT
                     GNVLDLGLVGLVQFVPMVALTLVVGHVADRYDRSRIVAICQAIEALAALSLAAAVAYG
                     VADRTWIFATAAALGAARAFENPTMQALLPQLVDRAIFPRATAWSASANQTAQIVGPA
                     VGGLLFLVHPIAPFVAAGSASSRRQSRSCAFHGGRPSDHRRLERRSADGALYRELHVR
                     GRRGPGVERRRASGARR"
     gene            complement(730..1935)
                     /locus_tag="PK264_08415"
     CDS             complement(730..1935)
                     /locus_tag="PK264_08415"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HRD75948.1"
                     /translation="MLEAYIYAGARTPFGRQDGALSRVRPDDLLGDVIKSVVAKSGFS
                     AQSVDDVNIGCAGQAGEDSRNVARHALLRAGLPIEVPGNTLNRLCGSGMNSLIAAAHA
                     ITCGEADVVVAGGVESMTRAPFVIGRTEGAFPKSVQWFNSSLGARFPNPRIEADFGGD
                     TMPQTADNMARDFQLTREACDSFALGSQQKFAAAQKAGFFKDEILAVSIPAAKKGAPD
                     TVVDADEHPRPETTMDILGKLRPIFKDGVTTAGNASGINDGAAALLIASRDAGDKAGV
                     KPRGRILSTGVAGVAPRIMGYGPVPAAQKALERAGLDLKDMDVIEVNEAFAAQVLGCL
                     KGLNVAFDDTRVNPNGGAIAVGHPLGASGSRIALTALRQLERTGGKYALISMCIGVGQ
                     GIAMIIERT"
     gene            2013..2378
                     /locus_tag="PK264_08420"
     CDS             2013..2378
                     /locus_tag="PK264_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD75949.1"
                     /translation="MAEVRYIVNDVPASIAFYTGALGFELIEQFGPAMAIVRRGDLDL
                     WLAGPLASASRPMPDGSRPVPGGFARIVLRVDDLDRQIAQLKGTGVRFRNDIVAGPGG
                     RQVLCLDPAGNLVELFERK"
     gene            complement(2392..2862)
                     /locus_tag="PK264_08425"
     CDS             complement(2392..2862)
                     /locus_tag="PK264_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75950.1"
                     /translation="MSSRITLASIVAATVASSLTAFVLSPATEVAADSATTSPSPPGC
                     SCSESPSDMRRRLWPQPKLADARPSTTLEPTDELGALEAVNEALEETGDGSSYVWHGP
                     NGRLSAVIQPTSSFRDRSGRICRHLHVLLNAGEVTRKAEGIACRSALGVWSLTG"
     gene            complement(2979..4139)
                     /gene="tgt"
                     /locus_tag="PK264_08430"
     CDS             complement(2979..4139)
                     /gene="tgt"
                     /locus_tag="PK264_08430"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRD75951.1"
                     /translation="MSEGGSDVSFRLLAEDGLARRGEIATPRGTIRTPAFMPVGTAAT
                     VKGVYADQVREAGADILLGNTYHLMLRPGAERIARLGGLHAFMRWERPILTDSGGFQV
                     MSLSKIRDLDEAGVTFQSHLDGSRHKLTPERSIEVQCLLGADIQMQLDECIAFPAEYA
                     EAKRAMELSLRWAERSKAAFQRYGKRGQALFGIVQGSTFSDLRRASAEALRALDLPGY
                     AVGGLAVGEGQEQMLCTLEATLPFLPREKPRYLMGVGTPLDLIEAVARGIDMFDCVMP
                     TRNGRHGHAFTWRGPVNLRNARHAEDPAPLDPESTCPAARDYSRAYLHHLVKSGELLG
                     SMLLSWSNTAFYQQLMAATREAITEGRFAAFAARMRVELRGGGSADDDAEQN"
     gene            complement(4144..4422)
                     /locus_tag="PK264_08435"
     CDS             complement(4144..4422)
                     /locus_tag="PK264_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HRD75952.1"
                     /translation="MSQKPSPATSAPTEGKPAQLLHLVFGGELTSLDKLEFADLAKLD
                     IVGIFPNYAEAQKAWRAAAQRTVDSAQTRYYVVHIHRLMTPDQPKPKG"
     gene            4630..5853
                     /locus_tag="PK264_08440"
     CDS             4630..5853
                     /locus_tag="PK264_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75953.1"
                     /translation="MTMWTSRVKVGLALGAVALSAAAVLTPIRAQQGTIKIGELNSYK
                     AQPAFLEPYRKGWQLAVEEINAAGGLLGKQLEVVSRDDTGVPGDAVRVAEELASRENV
                     VLLTGGFLSHVGLAISDFAKNRKILFVAAEPLTDKLVWEAGHRYTFRLRASTYMQTSM
                     LVAEAVKLKKKRWAIVYPNYEYGQSAVASFKALMKAAQPDVEFVAEQASPLGKIDAGA
                     VAQAIGDAKPDGIFNVTFGADLAKFVREGNTRSLFKDRVVLSLLSGEPEYLDPLKDET
                     PAGWIVTGYPWQAIDTPEHKAFVVAYQKKYNDYPRLGSVVGYAAMQTVAAAIKKAGST
                     DTDKLIAAMQGLELSTPFGKIVYRAGDHQSTMGAYVGRLAVKDGKGMMVDWRYEPGER
                     HLPSEEEGRKRRPAQ"
     gene            5876..7012
                     /locus_tag="PK264_08445"
     CDS             5876..7012
                     /locus_tag="PK264_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HRD75954.1"
                     /translation="MARAEPSFTIGIEEEYHLVDLRSRNLVSDPPKALIAEVEAALGG
                     HVASEFLRSQIEVGTPVCRTSAETRAELVRLRRALIDATGRHGLAPIAAATHPFARSL
                     DQQTTDKDRYRALARDLQGVGRRLVISGMHVHVGIEDDELRIDLMNQLRYFLPHLLML
                     ATSSPFFEGEDTGLKSYRLAIYNELPRTGLPGRFADWGEYRQTIDVLVQGGVIEDASK
                     VWWDIRPSARFATLEMRITDVCTRIEDAVCVAALYRCLCRMLYRLRRSNQSWRTYPVF
                     LLAENRWRAQRYGVDGSLFDFGKGTLVPFRDLIEEIIALVADDADAFGCRAEVEHARF
                     IAAGGTSADRQLAVYRARLASGSSPESALVAVADHLVAETCAGA"
     gene            complement(7041..8036)
                     /locus_tag="PK264_08450"
     CDS             complement(7041..8036)
                     /locus_tag="PK264_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRD75955.1"
                     /translation="MEALTIGLLIALALVLAAEFVNGWTDAPNAIATVISTGVMTPRQ
                     AIVMAVIMNTLGAAAGTAVAATVGKGIVAPSALTVASITATMVSIIIWGSLAARFGIP
                     VSKSHALLAGLAGAALAKGGVGALQWDGWLKVGIGLACSIGLGFVAAFLLGRLIARLA
                     ANAPPTRAKRFFDRAQMLSAAFMAFNHGLNDGQKFMGVFTLTLLAGGAIKAFHIDWWV
                     IIICALTMGAGTSAGGWKIIRTMGTKMTRITTWQGFAATTAASFTIFGASAYGVPLST
                     THTITSAVVGVGASKRTSDVRWGVLKKILLAWCATFPACALIAYVAALFANRVFG"
     assembly_gap    8148..8245
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8246..8697)
                     /locus_tag="PK264_08455"
     CDS             complement(<8246..8697)
                     /locus_tag="PK264_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRD75956.1"
                     /translation="MLKTMQGALAAFFGSSHNDRYAELLKGVSEVAIQCGRRLKETGG
                     QDVASIIELEHKADRLTDEIHELLDNSFIMRFDIPDAMRLADDIDDVVDGMRKVALHI
                     DGYKKFLSKMPPEAIELMSMSIDMMVKVDGLVAMLALLSGVAISGSLDP"
     gene            complement(8874..9560)
                     /locus_tag="PK264_08460"
     CDS             complement(8874..9560)
                     /locus_tag="PK264_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRD75957.1"
                     /translation="MLEDPPLLTIARSWQRPEASVIARLKDVQTGQAVDAMEGRGGLA
                     CEIKPLDPASARMLGPAVTCETGPNDNLAILGALAVAKAGDVIVAATEGFAHSAVVGD
                     NVALMAKRKGIAGIVVDGMMRDLVGLLPVGLPLFCRGITPNSCVRSGPGRVGLPIVAG
                     GVAIRSGDIVIGDRDGVVVIPQAEIAALQPKIDRILAAEADSQAKIKAGFTNLGGIDD
                     LLKSSQVRWV"
     gene            9692..10537
                     /locus_tag="PK264_08465"
     CDS             9692..10537
                     /locus_tag="PK264_08465"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD75958.1"
                     /translation="MADEKPNHKADAFIKEVEEDLRREQLEKLWENYGVYAIGVAALI
                     VVGIGGYRFMETRSVGETGAAYSAATRLVEQKKTDEALKAYDALAKGSSASYATLSKL
                     RIAGDLGRSGKAKEAITLFDEIAKGGGDQLLASYASLQSAMLMVDTAGWTEIETRLKP
                     LIDGTSPWRHMARETLGIAAIKAGRTSDARRHLGQVIADQNAPPALIERARTLMAIVT
                     EGELGKRVPRTEAPKAPAPVAPVKAADPKPAPAPAAGPEAKTASPAKPEDGKPIPPKD
                     GTAKK"
     gene            10549..11907
                     /locus_tag="PK264_08470"
     CDS             10549..11907
                     /locus_tag="PK264_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRD75959.1"
                     /translation="MVERSKPSSLALRGSCLGAIVASGLLAAGCSDSLPSMPRFSDLN
                     PFAEKQVPLPGKRVPVSLAEGKMGADLASGDRPVILPPTIANDAWTQPGGVATNAPGH
                     LALGSSIKVTWSSDAGQGSSSRGRLISSPIVAEGRVFTLDTAALVTAFNAASGAIVWR
                     SSLTPENEKGGKGAGGGLAFEGGRLYAATGYGNVVAIDPASGKKLWEKSLGSPVRASP
                     TAHGGRVYILALDGVLYTLNGADGTEAWVHKGLPEKASIVSNSSPAVDGTSVVIPYPT
                     GELVTVNATNGAQTWSESLARARVASSLTAMSDAARPAIDNGVVFAVGHAGRMVAASA
                     RTGERLWTINVPSIQAPWVAGETVFVVDTSGQLMALTRREGRAVWTVKLPGGSIWSGP
                     VLAGGRLWLVSDKGTLVGVEAVTGRVDVQQNLGAPSFISPIVAGGRMYVLTDKARLIA
                     LN"
     gene            11929..12471
                     /gene="moaB"
                     /locus_tag="PK264_08475"
     CDS             11929..12471
                     /gene="moaB"
                     /locus_tag="PK264_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HRD75960.1"
                     /translation="MAKIDESRPFIPVRIAVLTVSDTRSLEDDKSGTTLVGLLTEAGH
                     TLADRAIVTDDVAKIRARVLGWIADPAIDVVITTGGTGFTGRDVTPEAIRPLFEKEID
                     GFSTVFHMISFQKIETSTIQSRACGGLAHGTYIFCLPGSPGACRDGWNAILRFQLDNR
                     HRPCNFVEIMPRLEEHRRKG"
     gene            12725..13006
                     /locus_tag="PK264_08480"
     CDS             12725..13006
                     /locus_tag="PK264_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein usg"
                     /protein_id="HRD75961.1"
                     /translation="MPQRDGDKDFTAQLDGFSLTTAEILYHLPDHPSLLQSYLWQEYD
                     MAPKFPKLKGFLDFWSRNLDGRLNRIRVMHRSLITPREYRLLAGELKLH"
     gene            complement(13031..13801)
                     /gene="ubiE"
                     /locus_tag="PK264_08485"
     CDS             complement(13031..13801)
                     /gene="ubiE"
                     /locus_tag="PK264_08485"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HRD75962.1"
                     /translation="MSGNDQDQASFGFRDVPLGERQKLVNEVFRSVAERYDLMNDLMS
                     GGLHRLWKDDLVNWLAPSETRPMRLLDVAGGTGDVAFRFCSASRGSADGPDAIVCDLS
                     PEMVGVGRRRADERGLASRVGFVVGNAEALPFPDRSFDAYTIAFGIRNVTRIDKALAE
                     AFRILRLGGRFMCLEFSTVDVPMLDRLYDFHSFEVIPRLGGLAAGAEEPYRYLVESIR
                     KFPRQEPFAAMIRGAGFGRVSFRNLTGGIAAIHSGWKL"
     gene            complement(13822..14571)
                     /locus_tag="PK264_08490"
     CDS             complement(13822..14571)
                     /locus_tag="PK264_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HRD75963.1"
                     /translation="MSRIPLMPKATAVWLVENTSLSFEQIAAFCGLHNLEVKGIADGD
                     VAQGIKGMDPISSGQLTREEIKRCEAEPSAQLRLATTKVEIPVMKRKRGPRYTPVSRR
                     QDRPNAVLWLLKNHPELKDSQIIRLVGTTKPTIAAIRERQHWNSQNLQPQDPVTLGLC
                     SQIDLDSEVAKGAKRAGVVQPVDVSPAPERAGTLLPASATIPGLGPQPDIIEPPDADR
                     RAQEEAAEEERMLERLRSMGGGTEADASKPE"
     gene            complement(14829..15914)
                     /locus_tag="PK264_08495"
     CDS             complement(14829..15914)
                     /locus_tag="PK264_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75964.1"
                     /translation="MTELDTAAARRMGQRIAYAAIVIASLALTGCETASNILGTGPGP
                     EPVAQPLPAPAKIANKVAIAPVVGAPDAVGRQIVETLASATEQRGVVIAKTKDERSDF
                     VLRGYLVASKEKAIAKIAYIWDVTDPTGKRVNRITGEEVLPNPATREPWTLVTPDVTK
                     RIADKTSASLAAWLQSQTAVAGAASAQPAGPAGVGGQPLPQPVAATAMPPPLAPVTPP
                     PPATPVPAVATGPAPGPIAALPKGDVASLAPTVAGAPGDGNSTLAAALLKELRRNGVV
                     AGEGRATSYRIVGRVVMGAPREGKQTIQIDWQVIDPTGKSLGTVSQKNEVPQSSLDGA
                     WGQTADAAAAAAAQGILKLLPPVTRTQ"
     gene            16439..16777
                     /locus_tag="PK264_08500"
     CDS             16439..16777
                     /locus_tag="PK264_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRD75965.1"
                     /translation="MTYIVNENCIKCKFTDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPVDAIKPDTEPGLEKWLELNRTFSEQWPNITAKKPQPPDADKYRDEKGKYEK
                     YFTDKPGEGD"
     assembly_gap    16998..17040
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17041..>17403)
                     /locus_tag="PK264_08505"
     CDS             complement(<17041..>17403)
                     /locus_tag="PK264_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75966.1"
                     /translation="AAVEPPRSGAEQPRESANAESEQRIALIKSIQKELKRVGCSPGA
                     ENGRWSASVVKAIENFSRITKMKLDSASPTDDILSALRGRSSRVCPLVCDDDEIVVGN
                     ECRPKPAPVVKQKVATPKP"
BASE COUNT         2943 a         5675 c         5815 g         2829 t
ORIGIN      
        1 cgtcgggcac ctgaagcccg gcgtcgttca acacctggac cgcgccgacc ccggacatga
       61 agctcgcgat ataaagcgcc gtcggcagat cgcctttcga ggcgtcgatg atcgctcggg
      121 cggccgccat ggaaggcgca ggatcgcgat tgccgccgcg aggaagccga gccggccgcg
      181 acaaacggtg cgattggatg cacgaggaag aggagaccgc cgaccgccgg tccgacgatc
      241 tgcgccgtct ggttggccga ggccgaccag gccgtcgccc gggggaagat cgcccggtcg
      301 acgagctgcg gcagcagggc ctgcatcgtc gggttctcga aggcgcgtgc ggcgccgagc
      361 gccgcggcgg tggcaaagat ccacgtccga tccgccaccc cgtaagcaac cgccgccgcg
      421 agcgagagcg ctgccaaggc ctctatcgcc tggcagatcg caactatgcg gctccggtcg
      481 tagcgatcgg ctacatggcc gacgaccaga gtgagtgcga ccatcggcac gaactgaacg
      541 aggccgacga ggcccaagtc cagcacgttc cccgtaagct cgtagagctt ccagccgatc
      601 gcaatcgaca aagcctgata ggcgatcgtc gcgatgacgc gggctgccca gaacgtccgg
      661 aacgccggca ccgagaaagg cgacacctcg gaggccgccg tcatgagcgg cgcccccctg
      721 cgcgagcgct catgtgcgct cgattatcat ggcgatgccc tggccgacac cgatgcacat
      781 cgagatgagc gcatacttcc cgccggtccg ctcgagctgg cgaagcgcgg tcaatgcgat
      841 gcgcgagccc gaggcgccga gcgggtggcc gacggcgatg gcgccgccgt tcggattgac
      901 gcgcgtgtcg tcgaaggcaa cgttgaggcc cttcaggcag ccgagcacct gcgcggcgaa
      961 cgcctcgttc acctcgatca cgtccatgtc cttgaggtcg aggccggcgc gctcgagcgc
     1021 cttctgtgcg gcgggcaccg ggccatagcc catgatgcgc ggggcaacac ctgcgacgcc
     1081 cgtcgagagg atgcgaccac gcggcttcac gcccgccttg tcgccggcat cacggctcgc
     1141 gatgaggagg gcggcggcgc catcattgat gcccgaggca ttgccggcgg tcgtcacgcc
     1201 gtccttgaag atcggccgga gcttgccgag gatgtccatg gtcgtctcgg ggcgcggatg
     1261 ctcgtcggca tcgacgacgg tgtccggcgc gcccttcttg gcagcgggaa tggagactgc
     1321 gaggatctcg tccttgaaga acccggcctt ctgggccgct gcaaacttct gctgcgagcc
     1381 gagcgcgaag ctatcgcagg cctcgcgcgt cagctgaaag tcgcgcgcca tgttgtcggc
     1441 cgtctgaggc atggtatcgc cgccgaaatc ggcctcgatg cgcggattgg gaaagcgcgc
     1501 gccgagcgag gagttgaacc actggacact cttcgggaag gcgccttccg tgcggccgat
     1561 gacgaagggc gcgcgcgtca tgctctcgac accacctgca accacgacgt cggcttcgcc
     1621 gcaggtgatg gcgtgagccg ccgcgatgag cgagttcatg cccgagccgc agagccggtt
     1681 gagcgtgttg ccgggcacct cgatgggaag acccgcgcgc agcagcgcgt gccgcgccac
     1741 gttgcggctg tcctcgcccg cctggccggc gcagccgatg ttgacgtcgt cgacggattg
     1801 ggccgagaag ccggacttcg ccacaacgct ctttatgaca tcgccgagga gatcgtcggg
     1861 gcgcacgcgg gagagcgcgc cgtcctggcg gccgaaaggc gtgcgggcgc cggcatagat
     1921 gtaggcttcg agcatcgggg cgttcctcgg ttcttgtcgt ctgctcgcga acgattagca
     1981 tcgctcaatg cagagcgaca agcgagggtg acatggccga ggttcgctac atcgtcaacg
     2041 acgttccagc gtcgatcgcg ttctacacgg gcgcgctcgg cttcgagctg atcgagcagt
     2101 tcggaccggc catggcgatc gtacggcgcg gcgatctcga cctctggctc gcggggccgc
     2161 tcgcgtcggc atcgcgtccc atgcccgacg gcagccgtcc cgtgccaggc gggttcgccc
     2221 gcatcgtgct gcgcgtcgac gatctcgatc ggcagatcgc gcagttgaag ggcacaggcg
     2281 tgcggttccg caacgacatc gtcgccgggc cgggcgggcg gcaggtcctt tgcctcgatc
     2341 cggccggcaa tctcgtcgag ctgttcgagc ggaagtgatc ggctctcggg attagcccgt
     2401 gagcgaccac acgccgagcg cgctgcggca ggcgatgcct tcggccttgc gagtgacctc
     2461 gccggcgttc aggagcacgt gcagatggcg gcagatgcgg ccacttctat cgcggaagga
     2521 ggaggtcggc tggatgacgg cggagaggcg cccgttcggt ccgtgccaga cgtagctcga
     2581 cccgtcgccg gtctcctcga gcgcctcgtt gacggcctcg agcgcgccga gttcatcggt
     2641 cggctcgagc gtggtcgacg gacgggcgtc tgcgagcttc ggctgcggcc agaggcggcg
     2701 gcgcatgtcg gacgggcttt ccgagcagct gcatccgggt gggcttggcg acgtcgtcgc
     2761 cgagtcggcc gcgacctcgg tcgcaggcga gagaacgaag gctgtcagcg acgaggcgac
     2821 cgtcgccgcg acgatcgagg cgagcgtgat acgcgagctc atggggctac tcgactaaac
     2881 gcaacaggac acacttggag caacaagctc ccggtcctga agtgtcgcac cgaggtgtaa
     2941 acgagtggtt agccggcgtc ctgaaatcgg cgtggccctc agttctgttc ggcgtcatca
     3001 tccgctgatc cacccccgcg cagctcgacg cgcatgcgcg ccgcgaaggc ggcaaaccgt
     3061 ccttcggtga tcgcttcacg ggtcgccgcc atcagctgct ggtagaaggc cgtgttcgac
     3121 caggagagta gcatcgagcc gagcagctcg cccgacttga cgaggtggtg gagataggct
     3181 cgcgagtagt cgcgcgccgc agggcaggtg ctctcgggat cgagcggggc cgggtcctcg
     3241 gcgtgacgag cattgcgcag gttgacgggg ccgcgccagg tgaaggcatg tccgtgccgg
     3301 ccattccgcg tcggcatcac gcaatcgaac atgtcgatgc cacgtgccac cgcctcgatc
     3361 aggtcgagcg gcgtgccgac gcccatgagg tagcgcggct tctcgcgcgg caggaacggc
     3421 aacgtcgcct cgagggtgca cagcatctgc tcctggccct cgccgacggc gagcccgccg
     3481 accgcatagc ccggcaggtc gagagcgcgc agcgcctcgg ccgaggcccg cctcaggtcg
     3541 gaaaacgtgc tcccttgcac gatgccgaag agggcctggc cgcgcttccc gtagcgctga
     3601 aacgctgcct tcgagcgctc ggcccagcgc agcgagagct ccatcgcccg cttcgcctcg
     3661 gcatactcgg ccgggaaggc aatgcactcg tcgagttgca tctggatgtc ggcgccgagc
     3721 aggcactgca cctcgatcga gcgctccggc gtgagcttgt ggcgcgagcc gtcgaggtgc
     3781 gactggaacg tgacgcccgc ctcgtcgagg tcacggatct tggagaggct catgacctgg
     3841 aagccgccgg aatccgtgag aattggcctt tcccagcgca tgaaggcatg cagcccgccg
     3901 agccgcgcga tccgctccgc acccgggcgg agcatcaggt ggtaggtgtt gccgagcagg
     3961 atgtcggcgc cggcctcgcg tacctggtcg gcatagacgc ccttcacggt cgccgccgtg
     4021 ccgacgggca tgaatgcggg cgtgcggatc gtgccgcgcg gcgtcgcgat ctcgccgcgg
     4081 cgtgcgagcc cgtcctcggc cagcagtcga aacgatacgt ccgagccgcc ctccgacacg
     4141 cgctcagccc ttcggcttcg gctggtccgg cgtcatcagg cgatgaatgt ggacgacgta
     4201 atagcgcgtc tgggcgctgt cgaccgtcct ctgcgccgcg gcccgccacg ccttctgcgc
     4261 ctcggcatag ttcgggaaga tgccgacgat gtcgagcttc gcgagatcgg cgaactcgag
     4321 cttgtcgagc gaggtgagct cgccgccgaa aacgagatgg aggagctggg cgggcttgcc
     4381 ctcggtcggc gccgaggttg cgggcgatgg cttctgagac atgtgagttc cggttctgag
     4441 gagcgtcgtc gatccggcat atgatccgct ctcgccgagg aggcaagccc gacgatcggg
     4501 caaagaggca cgtgaagtgc tcggggtaaa gcaagcctgc agctgttcgc cgagacattg
     4561 ccgcgatccg gtgctttact ggccacagtc attgggcgtc cgttcgggcg caactgggag
     4621 gcaagccgga tgacgatgtg gacatcgcgt gtgaaggtcg gtctagcact cggcgccgtg
     4681 gcgctctccg ctgcggcggt cctcacgccc attcgcgccc aacagggcac gatcaagatc
     4741 ggagagctca acagctacaa ggcgcagccg gccttcctcg agccgtaccg gaagggctgg
     4801 cagctcgccg tcgaggagat caacgccgcg ggcggcctcc tcggcaagca gctcgaagtc
     4861 gtctcgcgcg acgatacggg cgtgccgggt gacgccgtgc gcgtcgccga ggagttggca
     4921 agccgcgaga acgtcgtcct gctgacgggc ggattcctct cgcacgtcgg ccttgcgatc
     4981 tcggacttcg ccaagaaccg caagatactg ttcgtcgccg ccgagccctt gaccgacaag
     5041 ctcgtctggg aagcgggcca ccgctatacg tttcgactac gcgcctcgac ctacatgcag
     5101 acgtcgatgc tcgtcgccga ggccgtgaag ctcaagaaga agcgatgggc gatcgtctac
     5161 ccgaactacg agtacgggca gtcggcggtt gcctcgttca aggcgctcat gaaggccgcg
     5221 caacccgatg tcgagttcgt cgccgagcag gcctcgcccc tcggcaagat cgacgctggc
     5281 gccgtcgcgc aggcaatcgg cgacgccaag cccgacggca tcttcaacgt gacgttcggt
     5341 gccgacctcg ccaagttcgt gcgcgaaggc aatacgcgca gcctcttcaa ggaccgcgtg
     5401 gtgctgagcc tgctctcggg cgagcctgaa tacctcgatc cgctgaagga cgagacgccg
     5461 gccggctgga tcgtgacggg ctatccctgg caggcgatcg atacgcccga gcacaaggcc
     5521 ttcgtcgtcg cctaccagaa gaagtacaac gactatccgc gcctcggctc cgtcgtcggc
     5581 tacgccgcca tgcagacggt cgccgcagcg atcaagaagg ccggctccac cgacaccgac
     5641 aagctgatcg cggcgatgca ggggctcgag ctctcgacgc ccttcggcaa gatcgtctac
     5701 cgggcgggcg atcatcagtc gacgatgggc gcctacgttg gtcgcctcgc cgtcaaggac
     5761 ggcaagggca tgatggtcga ctggcgctac gagccgggcg agcggcacct gccgagtgag
     5821 gaggaaggcc gcaagcggcg gcccgcgcag taaccggcag ggctcgagac ccgagatggc
     5881 gcgcgccgag ccgagcttca cgatcgggat cgaggaggag tatcacctcg tcgatctccg
     5941 ctcgcgcaat ctggtgagcg acccgccgaa ggcgctgatc gccgaggtcg aggcggcgct
     6001 cggcggtcac gtcgcgagcg agttcctgcg ctcgcagatc gaggtcggca cgcctgtctg
     6061 caggacgagt gctgagacgc gggccgagct tgtcaggctg cgccgcgcgc tgatcgacgc
     6121 aaccggccga cacggcctcg cgccgatcgc cgcggcgacg catccattcg cgcgctcgct
     6181 cgaccagcag acgacggaca aggatcgcta ccgcgcactc gcccgcgatc tgcaaggcgt
     6241 cggccgccgg ctcgtcatct ccggcatgca cgtccacgtc ggcatagagg acgacgagct
     6301 tcgcatcgat ctcatgaacc agctgcggta cttcctgccg catctcctca tgctcgcgac
     6361 gtcgtcgccc ttcttcgagg gcgaggacac gggcctcaaa agctatcggc tggcgatcta
     6421 caacgagctg ccgcgcacgg gccttcccgg ccggtttgcc gactggggcg agtaccgcca
     6481 gacaatcgac gtcctggtgc agggcggtgt catcgaggac gccagcaagg tgtggtggga
     6541 catccgcccg tcggcgcgat ttgccaccct cgagatgcgc atcacggacg tgtgcacgcg
     6601 tatcgaggat gcggtgtgcg tggcggcact ctatcgctgc ctgtgccgca tgctctatcg
     6661 gctccgtcgc tcgaaccaga gctggcgtac ctatcccgtg ttcctgctcg ccgagaaccg
     6721 ctggcgggcg cagcgctacg gcgtggacgg gagcctcttc gacttcggca agggcacgct
     6781 cgtgccgttc cgcgacctga tcgaggagat catcgcgctg gttgccgatg acgccgacgc
     6841 tttcggatgc cgcgccgagg tcgagcatgc gcgcttcatc gcggccggcg gcacgagcgc
     6901 ggaccgacag ctcgcggtgt atcgggcgcg tcttgcatca ggaagctcgc ccgagtcagc
     6961 tctggtggcc gttgccgatc acctcgtcgc cgagacctgc gccggcgctt gacgcgattc
     7021 tgcgcggcac ctcaccgggt tcagccgaag acccggttcg cgaaaagagc tgcgacatag
     7081 gcgatgagtg cgcaggcggg gaatgtcgca caccaggcga gcaggatctt cttcaacacg
     7141 ccccagcgga catccgaggt gcgcttgctc gcgccgacgc cgacgaccgc gctggtgatg
     7201 gtgtgcgtcg tcgacaatgg aacgccgtag gccgacgcgc cgaagatggt gaacgaggcg
     7261 gccgtggtcg cggcgaagcc ctgccaggtg gtgatgcgcg tcatcttcgt gcccatggta
     7321 cggatgatct tccagccgcc agcgctcgtg ccggcgccca tcgtcaaggc gcagatgatg
     7381 atcacccacc agtcgatgtg aaacgccttg attgccccgc cagcaagcag cgtgagcgtg
     7441 aagacgccca tgaacttctg tccatcgttg agaccgtgat taaaggccat gaaggcggcc
     7501 gaaagcatct gcgcgcgatc gaagaagcgt ttcgcgcgcg tcggcggcgc gttggcggcg
     7561 aggcgcgcaa tgaggcggcc gaggagaaag gccgcgacga agccgagccc gatcgaacag
     7621 gcgaggccga tacctacttt cagccagccg tcccattgca gcgcgccgac cccccctttg
     7681 gcgagagcgg caccggcaag gccggcgagc agcgcgtgcg acttgctgac cgggatgccg
     7741 aaacgcgcgg caaggctgcc ccagatgatg atcgagacca tcgtcgcagt gatcgaggcg
     7801 acagtcagcg cggagggcgc aacgatgccc ttgccgaccg tcgccgcgac ggctgtgccg
     7861 gcggccgcgc cgagcgtgtt catgatcacg gccatgacga tcgcctgccg tggcgtcatg
     7921 acgcctgtcg agatgacggt cgcgatggcg ttgggcgcat ccgtccagcc gttgacgaac
     7981 tcagcagcaa gaacgagcgc gagcgcgatc aggaggccga tggtcagggc ctccattcac
     8041 gcctccttgc gcgcaagcga caggatgagc ttggcgcaat ggttggcgtc gtccgtgatc
     8101 tgctcgagga gatggaagag ctggtgccag gcgatgaagc ccaagccnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnggatc gagggaaccg gagatggcga caccggacag
     8281 gagggcgagc atggcgacga ggccatccac tttcaccatc atgtcgatgc tcatggacat
     8341 gagctcgatc gcctcgggcg gcatctttga gaggaacttc ttgtagccgt cgatatgcag
     8401 cgcgaccttg cgcatgccgt cgacgacatc atcgatgtcg tcggcgagac gcatggcatc
     8461 ggggatgtcg aagcgcatga tgaaggagtt gtcgagcagc tcgtgaatct cgtccgtgag
     8521 ccgatcggcc ttgtgctcga gctcgatgat ggaggcgacg tcctgcccac cggtctcctt
     8581 caggcggcga ccgcactgga tcgccacttc tgagacacct ttgaggagtt cggcgtagcg
     8641 atcattgtgc gagctgccga aaaaggccgc cagcgcgcct tgcatcgtct tgagcacgtg
     8701 cccccctttc atcgcgcatc cggtaggctg ggcgatcggg aagctggtag gcgcggattc
     8761 gcctccaaac aagcacggat gtcacaaaac tgtcatgtca gcgtcaggcg gccgctggca
     8821 acgatctgct cgctaggcga gcgtgtttct gatgtcggtg cgccgccggc ctctcagacc
     8881 catctcacct gcgacgactt caacagatcg tcgatcccgc cgaggtttgt gaagcccgcc
     8941 ttgatcttcg cctgcgagtc ggcctcggcg gcgaggatcc ggtcgatctt cggctggagg
     9001 gcggcaattt cggcctgcgg gatcacgacg acgccatcgc gatcgccgat cacgatatcg
     9061 cccgagcgga tcgcgacgcc accggcgacg atcggcaggc cgacgcgtcc cggcccgctt
     9121 cgcacgcacg agttgggcgt gatgccgcgg cagaagagtg gcagcccgac cggcaggagg
     9181 ccaacaaggt cacgcatcat gccgtcgacg acgatcccgg cgatgccctt gcgcttggcc
     9241 atcagcgcaa cgttgtcccc gaccacggcc gagtgggcga agccctcggt ggcggcaacg
     9301 atcacatcac cggccttggc gacggcaagg gcaccgagaa tggccaagtt gtcgttgggg
     9361 cccgtctcgc aggtgacggc cggcccgagc atgcgggccg aggcgggatc gagcggcttg
     9421 atctcgcacg ccagcccgcc gcgcccctcc atggcgtcga cggcctgccc cgtctgcaca
     9481 tccttgaggc gggcgataac actcgcctcg gggcgctgcc aggagcgggc gatcgtgagg
     9541 aggggcgggt cttcgagcat cgttcacact cccgagtttg ccatatcgat agtcgatcac
     9601 cggccgtatt ggccgcacac ctgctttgcc ttgccgggaa ggcaggctat gaagggccgc
     9661 aaaccgctga cccgaggaac agagccccac gatggccgac gaaaagccga accacaaggc
     9721 cgatgccttc atcaaggagg tcgaggagga tcttcgccgc gagcagctcg agaagctctg
     9781 ggagaattat ggcgtctacg ccatcggcgt agcggcgctg atcgtcgtcg gcatcggcgg
     9841 ctatcgcttc atggagacgc ggtccgtcgg cgagacgggg gccgcctatt ccgccgcgac
     9901 ccgcctcgtc gaacagaaga agactgacga ggccctcaag gcctacgatg cgctcgccaa
     9961 gggctcctcg gcgagctatg cgacgctgtc gaagctcagg atcgccggag acctcggccg
    10021 ctccggcaag gctaaggagg ccatcaccct cttcgacgag atcgccaagg gcggcggcga
    10081 ccagttgctc gcgagctacg ccagcctgca aagcgccatg ttgatggtgg atacggcggg
    10141 ctggacagag atcgagactc gcctcaagcc gttgatcgat ggcacgtcgc cctggcggca
    10201 catggcccgg gagacgcttg gcatcgctgc gatcaaggcc gggaggacgt cggacgcccg
    10261 gcgccacttg ggccaggtga tcgccgatca gaacgcaccg ccagcgctca tcgagcgtgc
    10321 acggacgctg atggcgatcg tgacggaagg ggagctcggc aagcgagttc ccagaaccga
    10381 ggccccgaag gccccggcgc cggtcgcacc ggtcaaggct gccgatccga agcccgcccc
    10441 ggcgccggca gcgggtccgg aggctaagac cgcgagcccg gcgaaaccag aggacggcaa
    10501 gcccattcca ccaaaggatg ggacggccaa gaaatagggg atccgccgat ggtcgagcgg
    10561 tcgaagccga gctccctcgc cctccgcggc tcctgcctcg gcgcgatcgt cgcatcgggc
    10621 ctgctggccg ccggctgctc ggacagcctg ccgagcatgc cccgcttctc cgatctgaat
    10681 ccgttcgccg aaaagcaggt gccgctgcca ggaaagcgcg ttccggtgtc gctcgcggaa
    10741 ggcaagatgg gcgccgacct cgcctccggc gaccgccccg tcatcctgcc gccgaccatc
    10801 gccaacgatg cctggacgca acccggcggc gttgccacca acgctccggg gcatctggcc
    10861 ctcggcagtt cgatcaaggt cacctggtcg agcgatgccg ggcaaggctc gagttcgcgc
    10921 ggccgcctga tctcttcacc gatcgtcgcc gagggacgcg tcttcacgct cgacaccgcc
    10981 gcgctcgtca ctgccttcaa tgccgcgagt ggcgcaatcg tctggcgatc gtcgctgacc
    11041 cccgagaacg agaagggcgg caagggcgcg ggcggcggtc tggctttcga gggtggccgg
    11101 ctctatgcgg ccacgggcta cggcaatgtc gtcgcgatcg accccgcgag cggcaagaag
    11161 ctctgggaga agagtctcgg cagcccggta cgggcgtcgc ccacggcaca tggcgggcgc
    11221 gtctatatcc tcgcgctgga cggggtgctc tacacgctga acggcgccga tggcaccgag
    11281 gcctgggttc acaaaggcct gcccgagaaa gcgagcatcg tctcgaactc gagcccggcg
    11341 gtcgacggca cctcggtcgt catcccatac ccgacgggtg agctcgtcac ggtcaacgcg
    11401 acgaacggcg cgcagacgtg gtcggagtcg cttgcgcgcg cgcgcgtcgc ctcgtcactg
    11461 acggccatgt cggatgccgc ccggccggcg atcgacaatg gtgtcgtgtt cgcggttgga
    11521 catgccggtc ggatggtcgc cgcctcggcc cggaccggcg agcggctgtg gaccatcaac
    11581 gtgccgagca tccaggcccc ttgggtcgcc ggcgagacgg tgttcgtcgt cgatacttcg
    11641 gggcagctga tggctctcac gcggcgtgag ggccgcgcgg tctggaccgt gaagctgccg
    11701 ggcggctcga tctggtccgg tccggtgctg gccgggggac gcctatggct cgtctccgac
    11761 aagggcaccc ttgtcggcgt cgaggccgtg acggggcgag tcgatgttca acagaacctc
    11821 ggcgccccct ctttcatctc gccgatcgtt gccggcggcc gcatgtacgt gctgacggac
    11881 aaggcgcgcc tcatcgccct caactgaggg ctccatccga gtatctccat ggccaagatc
    11941 gacgagagcc gcccgttcat tccggtccgc atcgccgtct tgaccgtctc ggatacgcgc
    12001 tcgctcgagg acgacaagtc cggcaccaca ctcgtggggc tcttgaccga ggcaggccat
    12061 acgctcgccg accgcgccat cgtaacggac gacgtcgcca agatccgagc gcgcgtgctc
    12121 ggctggatcg ccgaccccgc gatcgacgtg gtcatcacca ccggcggcac cggatttacg
    12181 ggccgtgacg taacgcccga agcaatccgc ccgctcttcg agaaagagat tgacggcttc
    12241 tcgaccgttt ttcacatgat ctccttccag aagatcgaaa cctcgaccat ccagtcgcgc
    12301 gcctgcggcg gcctcgcgca cgggacctat atcttctgcc tgccgggctc acccggcgct
    12361 tgccgcgacg gctggaatgc catcctgcgc ttccagctcg acaaccgtca ccgcccctgc
    12421 aatttcgtcg agatcatgcc ccggctcgag gagcatcggc ggaagggctg aacgccagca
    12481 aaggcacttc gcgatcctgg ccctctgacc cggccccctg agttccatgc ccgctaccgc
    12541 gccgcaatag gttcgtgctg cgtcgcgaga tggcagcagc attcgtggtg aagtgccagc
    12601 gctctgattt tcttcaattt tttagttgtt tggcgaccac gctcggttac cctttggtct
    12661 ggctgacgca tgagtcgtca gatcgagatg gagtgagcat tccagaacca aggtgagaca
    12721 acgcatgcca cagagagacg gcgacaagga cttcacggcg caactcgacg ggttctcgct
    12781 gaccacggcc gagatcctct accacctgcc ggatcatccg agcctgctgc agtcctacct
    12841 ctggcaagag tacgacatgg cgccgaagtt cccgaagctc aaggggttcc tcgatttctg
    12901 gtcgcgcaat ctcgacggca ggctcaaccg catccgcgtg atgcacaggt cgctgatcac
    12961 gccgcgagag taccgactgc tcgcgggcga gctcaagctg cattgagggg cagttcggcc
    13021 ggaacagaga tcagagcttc catccggaat gtatggcagc aatcccgccg gtcagattgc
    13081 gaaatgatac gcgcccgaat ccggcgccgc ggatcatggc cgcgaacggc tcctggcgcg
    13141 ggaacttgcg gatgctctcg acgaggtagc ggtagggctc ctcggcgccg gcggcgaggc
    13201 cgccaaggcg cggaatgacc tcgaaggaat ggaagtcgta gaggcgatcg agcatcggca
    13261 catcgaccgt agagaactcg aggcacatga agcggccacc gagccgcagg atgcggaacg
    13321 cctcggcgag cgccttgtcg atgcgggtca cgttgcggat gccgaaggcg atcgtataag
    13381 cgtcgaagga gcggtcggga aacggcagcg cctcggcatt gccgacgacg aagccgacgc
    13441 gactcgcgag gccgcgctcg tcggcacggc gccgaccgac accgaccatc tcggggctca
    13501 gatcgcagac gatcgcatcc ggtccgtcgg cgctcccgcg cgatgcactg caaaagcgga
    13561 acgcgacgtc acccgtgccg ccggcaacgt caagcaatcg catgggccgc gtctcggatg
    13621 gcgcgagcca gttgacgagg tcgtccttcc agagccgatg caggccgccg gacatcaggt
    13681 cgttcatcag gtcatagcgc tcggcgaccg agcggaagac ctcgttgacg agcttctggc
    13741 gctcgcccag cggcacgtcg cggaagccga acgatgcctg gtcctggtca ttcccgctca
    13801 tcggcgccac gcctcgagaa gtcattccgg cttgctggcg tcggcttcgg tgccgcctcc
    13861 catggagcgg agccgctcga gcatgcgctc ctcctcggca gcttcctcct gcgcgcgccg
    13921 gtcggcatcc ggcggctcga tgatgtcggg ttgcggaccg aggcccggaa tcgtcgcgga
    13981 cgctggcagc agcgtgccgg cgcgctcggg cgcgggcgac acatcgacgg gctgcacgac
    14041 gccggcgcgc ttggcgccct tcgcgacttc cgagtcgaga tcgatctgcg agcagaggcc
    14101 aagcgtcacc gggtcctgcg gctgcaggtt ctgcgagttc cagtgctggc gctcgcggat
    14161 cgcggcgatg gtcggcttgg tcgtgccgac gaggcggatg atctggctgt ccttgagctc
    14221 ggggtggttc ttgaggagcc agagcacggc gttcgggcga tcctggcggc gcgagacggg
    14281 cgtgtagcgc ggtccgcgct tgcgcttcat gacggggatc tcgaccttgg tcgtggcgag
    14341 gcgcagctga gctgacggct cggcctcgca gcgcttgatc tcctcgcgcg tcagctgccc
    14401 cgacgagatc gggtccatgc ccttgatacc ctgcgcgacg tcgccgtcgg caatgccctt
    14461 cacctcgagg ttgtggaggc cgcagaacgc cgcgatctgc tcgaaggaga ggctcgtgtt
    14521 ctcgaccagc cagacggcgg tcgctttcgg catcaacggg atccggctca tggacttttc
    14581 cttttcgacc gacccggggg attttgtcct cgggcaggcc cagaatttgg agcttggggg
    14641 tttcaaggat cggtcatatc gcgaacgagc ggggagcgca acctgcgtac cacgctgcgt
    14701 gtgccgcgca attgagcccc tcggcggccc gaaaacagtg cggcgccggt caccttgtcg
    14761 gtgccggcgc cgcccatgtc acttttcagg cgaagtacga gccgaccgaa acctcgggcg
    14821 ggatcacgct attgtgttcg cgtgaccggt ggcagcagct tgagaatgcc ctgggcggcg
    14881 gccgcggccg ctgcatcggc cgtctgcccc caggcgccgt cgagcgagct ttgcggcacc
    14941 tcgttcttct gagacacggt gccgagcgac ttgcccgtcg ggtcgatcac ctgccaatcg
    15001 atctggatgg tctgcttgcc ttcgcgcggc gcgcccatga cgacacggcc gacgatccga
    15061 taggaggtcg ccctgccttc gccggccacg acgccgttgc ggcgcaactc cttcagcagc
    15121 gctgcagcaa gcgtcgagtt gccgtcaccc ggagcaccag caacagtcgg agcgagcgag
    15181 gcgacatcac ccttcggcag ggcggcaatc gggccgggcg ccggtcccgt cgcaacggcc
    15241 ggaactggcg ttgccggtgg aggcggtgtg accggggcca atggtggtgg catggccgtt
    15301 gctgccaccg gttgcggcaa cggctgcccg ccaacgcctg cggggcctgc cggttgcgcg
    15361 gatgctgctc ccgcaacggc cgtctgactc tgcagccacg ccgcgagcga ggccgatgtc
    15421 ttgtcggcga tgcgcttcgt cacgtcgggt gtgaccagcg tccagggctc gcgcgtggcc
    15481 ggattgggca gcacttcctc gcccgtaatg cggttcaccc gcttgccagt cggatcggtc
    15541 acgtcccaga tgtaggcgat cttcgcgatc gccttctcct tggaggcgac gagatagcct
    15601 cgaaggacaa agtcggaccg ctcgtccttc gtcttggcga tgacaacgcc acgctgctcg
    15661 gtggcgcttg cgagcgtctc gacgatctgg cggccgacgg cgtctggtgc tccgacgacg
    15721 ggagcaatcg cgaccttgtt ggcgatcttc gcaggcgcag gcagaggctg agccaccggc
    15781 tcggggcccg ggcccgttcc caatatattg ctcgccgtct cgcagcctgt gagcgcgagg
    15841 cttgcaataa cgatagccgc gtatgcgatg cgctgtccca tgcgccgcgc cgcagcagtg
    15901 tcgagttccg tcacgcgacg tccccctttg tttgacccgc tgtgagccgg cggccgacgt
    15961 acttaccctg cggccgattc ctattttgtc cggtacatga tgattcaatt agccgtttgg
    16021 cgccaatgcg taaagtgtga tcggctgcct tcctggccga ttgcctaacc gaacgccgca
    16081 cacatcccat tctgatgtgg acaacggcag aacagcaaca ccgcatccga gcgacgcccg
    16141 aggcaccggt cctggaccag tggcccgtcg cgccaaatcg caacatggct gcaagcgctc
    16201 tcgatttttg cgcgacacaa gggccactag gaactccaac ggcttagtgt ggcgttatgg
    16261 tcggaagttc gtatttcgag tgtgccgcac attgaatacg aacttccgaa ccgccacact
    16321 agcggcgatc ggcgcggctt gcactcggaa cccggcatgt tatgggagac accgatcagc
    16381 gcgagcgcca gagcaaagtg cgccttggcc tccccgtcag agatccggac ccggcatcat
    16441 gacctacatc gtcaatgaga actgcataaa gtgcaagttc accgactgcg ttgaagtctg
    16501 ccccgtcgac tgcttctacg agggcgagaa catgctcgtc attcatcccg acgagtgcat
    16561 cgattgcggc gtctgcgagc cggagtgccc tgttgacgcg atcaagcctg acaccgagcc
    16621 tgggctcgag aagtggctcg agctcaatcg caccttctcc gagcagtggc ccaatatcac
    16681 ggccaagaaa ccgcagcctc ccgacgccga caagtaccgc gacgagaagg gcaaatacga
    16741 gaagtacttc accgacaagc cgggcgaggg cgactgaagg actgccgaac ccgccagcag
    16801 tgccggccgt ggcacgcggc tcgcccgttc agcgctcatc gccccgagca gatgcgctgc
    16861 gagaagcgct gaatgcagac ccgaccggcg ccgtcgacgc acgtctgatg atcgaacatc
    16921 actggccggc agtcgccgcc aacccctggc gcagatcgtg acgacgcggc aggcttgggt
    16981 tccggcttcg gcgcgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 ggtttgggtg tcgcgacctt ctgctttacg acgggcgccg gcttcggccg gcactcgttg
    17101 ccgaccacga tctcgtcatc gtcgcagacg agcgggcaga cccggctcga acggccgcgg
    17161 agcgccgaca ggatgtcatc tgtcggcgat gccgagtcga gcttcatctt ggtgatgcgc
    17221 gagaaattct cgatcgcctt cacgacgctc gcgctccagc gaccgttctc ggcgcccgga
    17281 ctgcagccga cgcgcttcag ctccttctgg atcgacttga tgagcgcgat gcgctgctcg
    17341 ctctcggcat tggcggactc gcgcggctgc tcggcgcccg agcgtggcgg ctcgacggcc
    17401 gcc
//
LOCUS       DAORNH010000062        17325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2486_length_17325_cov_2.905385, whole genome shotgun sequence.
ACCESSION   DAORNH010000062 DAORNH010000000
VERSION     DAORNH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2486_length_17325_cov_2.905385"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(433..>1009)
                     /locus_tag="PK264_08510"
     CDS             complement(433..>1009)
                     /locus_tag="PK264_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75967.1"
                     /translation="SEGIHSAWHPDQFACTQDVPRVAYDPAKAKALLAEAGFPNGLDV
                     DLVAYREREYTEAVIGDLAKVGIRAKLVFLQYTAMVQQIHKGQIGFVHSTWGSGSVPD
                     VGASAGHFFAGSPDDMVKDAEVIRLIGEADRNVDPAQRAALWKQALTRISGEVFWLPL
                     YTYAKYYVYSKDLAFKPTSDELPQFWNVKWK"
     assembly_gap    1010..1022
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1023..1981)
                     /locus_tag="PK264_08515"
     CDS             complement(<1023..1981)
                     /locus_tag="PK264_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD75968.1"
                     /translation="MQLKMRGLVAGGLLAGIAALAPVPLEAGKANDTLVWASDRENPI
                     ADTNFLNTRENFIISFLIFDRLVLLDETYKPQPLLATGWKWVDNVTLDFDLRAGVKFH
                     SGKALDADDVVYSLNFVVDPANGSFSRSYLTWIKSVEKTGDLKVRINLTKPFPAALTF
                     LSGAGVIVPKGHYDAAPLKPDGKRDFGAVKPVGTGPYKLAEIKPGDFIHLVKNAEYFK
                     GGPKIAPVIGAIRFRTIKDQNTRLAEVMTGNVDWIWDVPKELAERVKSNPAVVVENAK
                     TMRFAYLGFDTRGTSATKTFMDKRVRLAVAHAINREAIVKNLVG"
     gene            complement(2070..2972)
                     /gene="serB"
                     /locus_tag="PK264_08520"
     CDS             complement(2070..2972)
                     /gene="serB"
                     /locus_tag="PK264_08520"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HRD75969.1"
                     /translation="MRFVVVVTGAPPGGEETEERIRALVAGEPRLDIAAARWLAPKEA
                     CELDIELAGSAARGFVDRLQPFAESARLDIAIVPAAGRRKALLISDMDSTIIGQECID
                     EMADVVGRKREVAAVTERAMRGELPFEAALEARLALLKGVTTVDLERVFERRISLMPG
                     ARTLVQTMRANGAYTALVSGGFTFFTSRVAKAVGFDMHRANTLELAGETMTGRVVQPI
                     LGREAKREMLEGLVSARGIAPAATLAVGDGANDLAMIRQAGLGVAFRAKPIVAAEAHA
                     AIRHGDLTALLYLQGYARSEFATV"
     gene            complement(2985..4307)
                     /locus_tag="PK264_08525"
     CDS             complement(2985..4307)
                     /locus_tag="PK264_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD75970.1"
                     /translation="MPTLARRPWVPPAVEDVQARYAASAATLGSAALQHRIEALADRN
                     REIHEKDCFNLNPATNTMNPRAEALLASGLGVRPSLGYAGDKYEMGLEACEEIEVITA
                     ELAAEVFGAKFAEIRVPSGAIANLYAFMSTCQPGDAVIVPPAEIGGHVTHHRDGAAGL
                     YGVAIHPAPIDAAHYTVDVARLAELAERVKPKLITIGASLNLLPHPVRELRAIADRVG
                     AKLLFDAAHMSGLIAGRAWQQPLAEGAHLMTMSTYKSLGGPPAGLVLTNDAKLAQRID
                     RIAFPGLTANFDVSKAAALAVTLLDWREYGRAYAAKMVEMAKALAEALAAEGLPVHRT
                     AGGFTTSHQFAIEAHRWGGGQAASKLLRRANILACGIGLPIAAVDGDNNGLRIGTPEI
                     VRWGFDVQDCAPLARLVARVLAGNEAPEAIAPDVTAMRHRFTKLSYVC"
     gene            4389..5207
                     /locus_tag="PK264_08530"
     CDS             4389..5207
                     /locus_tag="PK264_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD75971.1"
                     /translation="MADATRNQDIVVITGGSEGIGLAFARAYHDAGRPLLLIARSAER
                     LAAARSALGDTRVHTLALDITRDDALEAIDAALAGIGGQCDELVNSAGFGLSGNFASL
                     APGRLEALVTLNVTAAMRLMRHVLPGMIERRRGLIINVASLGGLVPGPHQAAYYASKA
                     FLISLSEAVAAEVKAHGVRVMALAPGPISTEFHRRMGSESDLYLKLLPVQRPGTVARW
                     ARLGAALGARVIVPGLLQSIAALTLRVLPHRLSIPIMAILLRPRGRARSMSTSH"
     gene            5380..6588
                     /locus_tag="PK264_08535"
     CDS             5380..6588
                     /locus_tag="PK264_08535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD75972.1"
                     /translation="MSRQPVHKPPVVIVTLTLDVGGTEQHIATVAPLLVERGWPVSVY
                     CLNRAGEQAELLRSRGVDVIAPPREAAGRQTPRAKRAVLSTLAAADLFRKYRRQKPAI
                     AHTFLTEPFLFAGPAGILARVPIRVMSRRELKATRTNWPGARAVERRLIARMAAVLGN
                     SRSVVAELIEDGAPRDRIGLIYNGIDVRRFSAGVDRERLRRDLGIAGDGRIAIMVANL
                     FPYKGQRELIEGFASVAASLPEPWTLVLVGRDEGDRAHLEARIAALGVGDRVRLVGQR
                     KDVPSLLAASDIGIHASRQEGFSNAVLEQMATGLPVIVTEAGGNTDAVTHDKTGLIVP
                     IEAPEALAAAISTLARDPARRTRLGAAARARIEKEFTLARVVDAYEALYMGLLAGRRP
                     AEIEGVGIGR"
     gene            complement(6595..7296)
                     /locus_tag="PK264_08540"
     CDS             complement(6595..7296)
                     /locus_tag="PK264_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD75973.1"
                     /translation="MPIFAARAARVSVQLVAAFVLTGALVATTAIAGDGKSAAAFVAG
                     PGCPGFPSDTARPTGTGQPPSVDYPVCADHQQRLQIALAGAAARKRLVLVEFGATWCS
                     SCKSLRRQFAGGAILGQRIAAHDLATLYDVVSIPVSMVHLGRRAAVPSGEAALGDVLT
                     RAPGAKLRAVPFLAVIERVWARNIDDLASSSTGEHDAKRIGELLVEAHDHVRGTGPAA
                     SEPGWLRRKLRRWLN"
     gene            complement(7375..7839)
                     /locus_tag="PK264_08545"
     CDS             complement(7375..7839)
                     /locus_tag="PK264_08545"
                     /inference="COORDINATES: protein motif:HMM:NF018482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRD75974.1"
                     /translation="MAATMAAAVLAAPASLAQSIEVIATSGEWRAYAHGTGARRICFA
                     AATAGTGAFAYITAWPKDGVRSEFSLKTASAARKGSEALVTVGREQFRLFTDADRAWV
                     GDVATEARLIEAMKKGARMTVQVTAEKGAAAPEVYSLSGMTQALLAMGQACQ"
     gene            complement(7890..9470)
                     /locus_tag="PK264_08550"
     CDS             complement(7890..9470)
                     /locus_tag="PK264_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HRD75975.1"
                     /translation="MGEHGSTANRGLDRITVPGNVTGVVSGGRVMSHALLEEQVAKAA
                     GGLAARGVGQGDCVAILMRNDPVFLVASFAAMRLGAYAVPLNWHFKGEEVGYILRDCA
                     AKVFVGHTDLLAAVAEAVPERVAVIDVPPPPEVRAAYVMGEERRLPRALGWEAFLGES
                     QPWTGPPVPPTQSMIYTSGTTGNPKGVRRAPPNAEQQAALERMRVRTYGMVPGSRALL
                     PGPLYHSAPNSFAVRGARIAGVMVLMPRFDPIELLETIERYRIEITFMVPTMFVRLLK
                     LPEEVRRRYDVSSLRFVMHAAAPCAPDVKRRMIDWWGPVINEFYGATESGAVTIVSSQ
                     EWLARPGTVGRPVEGASVRIYDDAGALVPAGGVGEVYTRLDPFNNFTYHNLPEKRREI
                     DRDGHITCGDVGYLDPEGYLFLCDRKRDMVISGGVNIYPAEIEAAAHTMPGVRDCAVF
                     GVPDDEFGEALIALVEPVAGSGLTVEAFRAHLAAHLADYKVPRKIELRAELPREDSGK
                     IFKRKLREPYWQGAGRRI"
     gene            9666..10247
                     /locus_tag="PK264_08555"
     CDS             9666..10247
                     /locus_tag="PK264_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD75976.1"
                     /translation="MSPALFLDRDGVVNVDRDYVFRREDFDWQPGIFDLARTAHRAGL
                     PIVIVTNQSGIGRGYYSEVDFQALTTWMRTRFEDEGAPIRAVYHSPHHPEAARPEYRA
                     ADHPWRKPRPGMLLAARDEHGLDLVNSIMIGDRVSDIEAGAAAGVGTLVLVSEHSAEL
                     STRPTHRFTTILETATWFAGEMALRDLALTRNR"
     gene            complement(10231..10893)
                     /locus_tag="PK264_08560"
     CDS             complement(10231..10893)
                     /locus_tag="PK264_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD75977.1"
                     /translation="MALREFVSLVHKSTKRDYLARVTQRDKAEVADLAVKFDYDYWDG
                     GRDTGYGGYRYDGRWRKVADAMVAAYGIKPGMRILDVGSGKGFLLHDFREAVPGVEVA
                     GIDISKYAVEHTMESVKPFCKVGPAHKLPWPDKHFDLVVSINTLHNLYIQELWPAFKE
                     IERVGRGARYVCVEGYRNEREKVNLMYWQLTCRAFHTPDEWAFVFKETGYTGDHEFIY
                     FE"
     assembly_gap    11068..11088
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11089..>11409)
                     /locus_tag="PK264_08565"
     CDS             complement(<11089..>11409)
                     /locus_tag="PK264_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRD75978.1"
                     /translation="ELVKKRLVPAWQRVRARIEQGAVRASLDLARPLDHHEQCLSPSD
                     FGFHNALSDAGRVTFIDFEYAGRDDPAKLASDFCCQPEIPVPAEQHEAFVTALARRLE
                     LGES"
     assembly_gap    11410..11476
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11477..11923)
                     /locus_tag="PK264_08570"
     CDS             complement(<11477..11923)
                     /locus_tag="PK264_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRD75979.1"
                     /translation="MPAEATPLALAAELARRAGRAAPISTETLSGGKNNRVYRVALAD
                     GQSLLLKSYFRDLRDPRDRRKAEWEFLTLAWSRGVRAIPEPIATDVEAGAALMSFVPG
                     RKLAAGEIGAPEVEAALDFVVALNRPTPPVTALAPGSEACFSLGEHL"
     gene            complement(11925..12548)
                     /locus_tag="PK264_08575"
     CDS             complement(11925..12548)
                     /locus_tag="PK264_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75980.1"
                     /translation="MRIGIDFDNTLACYDGVFHAAALERELIPRDLPSDKTSVRDYLR
                     AEGQDPLFTELQGYVYGARMDLVAVYPGAFAALAALKSAGHELAIVSHKTREPFAGPR
                     YDLHAAARGFLVAQRLAGPGGLIDDARVHFELTKEAKVARAAALGCEVFIDDLPEILA
                     MPGYPPGLRAILFDPDGHYDGGRWKQHSFERHRSWAEIGRALGVGAA"
     gene            complement(12562..13602)
                     /locus_tag="PK264_08580"
     CDS             complement(12562..13602)
                     /locus_tag="PK264_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD75981.1"
                     /translation="MKTEAAILVELGKPLVLAELEIPALKTGQVLVEIAYSGACGSQV
                     NEAVGNKGEDKWLPHCLGHEGTGTVVETGGAVTKVKAGDKVVLSWLKGSGIEAGGCVY
                     TWGGKKVNAGAVTTFQRHAVVSENRLTLVPAGLGMDVAILLGCAAPTGMGAVFNVLKA
                     QAGDAVAVFGTGGIGLNACMAAAFAGATPVIGIDLSVSRRDLAKTYGATHVIDPAAGD
                     VLAEIRKIAPGGVDLAVEATGIPSVMDQAINATRAQGGRAVVIGNAKHGAMLSLSPNI
                     FNQGKSLMGTWGGDSVPDRDYGRFGRLIASGRFPVRELLSKPYSLAQADQALKDLAAG
                     KVGRPLIDMSLK"
     gene            complement(13642..14565)
                     /locus_tag="PK264_08585"
     CDS             complement(13642..14565)
                     /locus_tag="PK264_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRD75982.1"
                     /translation="MRNAFADELTKLGNSDSRVVMLSGDIGNRLFDKFKDAHPSRFFN
                     CGVAEQNMMGVAAGMAMSGLRPVAYTITPFITSRCLEQIRTDACYHDLPVTIVAVGAG
                     LSYAGLGPTHHACEDMAFLRAIPNMVVCAPGDAWEVRGALRAALKQPKPVYIRMGKKG
                     EPVVHKGVPESFALGKAITIEEGRDVCLLSCGTMLPEAIEAGHRLKAKGLSARVVSFH
                     TVKPLDEACLAEAFAKFRLVATIEEHSLLGGFGSAVGEWMIDRAVRPKAFIRFGTPDA
                     FFKKSGEQEYAREELGLTGHQIADRVLKALG"
     gene            complement(14562..15404)
                     /locus_tag="PK264_08590"
     CDS             complement(14562..15404)
                     /locus_tag="PK264_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRD75983.1"
                     /translation="MTRPTGKALPDAAALKRKAAELRGKVVEMSHAAQAAHLASSLSC
                     CDVVAAAYWHVLAIDPKTPTDELRDRFILSKGHAAAALYAALAFKGFFPVEELATYCK
                     DGGRLAEHPPANLLPGVEAATGSLGHGLPIGCGMALSGRIKGQTFRVYALLSDGENNE
                     GSVWEAAMFAAAQKLENVCVVVDYNKWQATARSNETLMLAPLADKWRAFGWDAHEVDG
                     HDVGKLAELMQKVPNGSGKPVALIAHTVKGKGVSFMEDDNNWHYRAPTADEVVKARKE
                     LGLA"
     gene            complement(15476..16474)
                     /locus_tag="PK264_08595"
     CDS             complement(15476..16474)
                     /locus_tag="PK264_08595"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRD75984.1"
                     /translation="MSGAAKYLVLGSNSFSGATFCDHLAARGADVIATSRSEEAHPAL
                     LPYKWQKRPGKVRFERVDLNTDLDKLDRLFKAERPTHVVNFAAQSMVGESWQFPEHWM
                     MTNVVSLARLADRLRRYEGLARYVHVTTPEAYGSTEGWVREDAPFNPSTPYATSRAAG
                     DMHLRTYFANYQFPVLFTRAANVYGPGQQLYRIIPRTIVAAMGGQKLKLDGGGKSVRV
                     FIHMNDVSTATEAIAERGALGACYHISGYELVSIRTLVEMILARVGKRFEDCVEIGPE
                     RAGKDTAYTLDSFKLRTELGWRDAISLGQGIDDVIAWARRFEGDIAKLPTRYEHKA"
     gene            complement(16471..17124)
                     /locus_tag="PK264_08600"
     CDS             complement(16471..17124)
                     /locus_tag="PK264_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRD75985.1"
                     /translation="MTSRTEAMARPLPGATTHSGDSPARHFRGYSQRLSDVLLGFDWA
                     PVERLTADLLDCWETGRQVFLCGNGGSAGNANHLANDLMYPLSKTKGSGIRAHALSAN
                     PAILTCLGNDEGYDSIFAYQLAVQARKGDVLLAFSGSGNSMNILKAIEEARRIGMTSY
                     AVVGFTGGKAKAMADVPIHFQIDDMQIAEDTQTIICHMIVQWLYQRRDAVAARKAHA"
     gene            complement(17121..>17325)
                     /locus_tag="PK264_08605"
     CDS             complement(17121..>17325)
                     /locus_tag="PK264_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD75986.1"
                     /translation="GRFRLDSEIPISAHAARQAPSKLGLRPGETIAVEDAIKEMVGMY
                     RQGVLKENDRWYNLKWMQKEVVK"
BASE COUNT         2794 a         5928 c         5612 g         2890 t
ORIGIN      
        1 cccttcgccg gcgagtaggg cgtgacccgg ccgacacgga cggaggccgc ccaccgagtc
       61 caatgctatt tgggactatg cgatcaactc cttttcgagc tcggccggcg cgtgcaggtt
      121 cttgacgttg tcactcaatt caggccgcca gttccaatca ccctcaaggc gatccttgcc
      181 gaattggttc gggaatatgt ccgcctgcgc tggccgcgca ccatttgctg gcggtaaggg
      241 tggcagcctg cgggccgact tcggcagcga cggaagcgct gagcgtgccg ccgtgacctc
      301 ccggctcccg gctcggcgtg ctcccccggc tcgggggccg gggtcgcgct tggtggagat
      361 gccggcctgg ggcagcgagc aggccgcggt ccgccccgcc acttcatatg ccgggagcgc
      421 gggtgcaagc cctcacttcc acttcacgtt ccagaactgc ggcaactcgt cggacgtcgg
      481 cttgaaggcg aggtccttcg agtagacgta gtacttcgca tacgtgtaga gcggcagcca
      541 gaacacctcg cccgagatgc gcgtgagcgc ctgcttccac agggctgccc gctgcgccgg
      601 gtcgacgttg cggtcggcct cgccgatgag gcggatcacc tcggcgtcct tcaccatgtc
      661 gtcgggcgaa cctgcgaaga aatgaccggc gctggcgccg acgtccggca ccgagcccga
      721 gccccaggtg gagtgcacga agccgatctg ccccttgtgg atctgctgca ccatcgcggt
      781 gtactgcagg aatacaagct ttgcccggat gccgaccttg gcgagatcgc cgatcacggc
      841 ttcggtgtac tcgcgctcgc gataggcgac gagatcgacg tcgaggccgt tcgggaagcc
      901 cgcctcggcg agcagcgcct tggccttcgc cggatcgtag gcgacgcgcg gcacatcctg
      961 cgtgcaggcg aactgatcgg gatgccaggc cgagtggatc ccctcggagn nnnnnnnnnn
     1021 nnccgacgag gttcttcacg atcgcctcgc ggttgatcgc gtgggcgaca gcgagccgca
     1081 cgcgcttgtc catgaacgtc ttggtcgccg aggtgccgcg cgtatcgaag ccgagatagg
     1141 caaagcgcat ggtcttggcg ttctcgacca ccacggcagg attgctcttc actcgctcgg
     1201 cgagttcctt cggcacgtcc cagatccagt cgacattgcc ggtcatcact tcggcgagcc
     1261 gcgtgttctg gtccttgatg gtgcgaaagc ggatcgcccc gatcaccggc gcaatcttcg
     1321 gtccgccctt gaagtactcg gcattcttga cgaggtggat gaagtcgccc ggcttgatct
     1381 cggcgagctt gtaggggccg gtgccgacgg gcttcaccgc gccgaagtcg cgcttgccgt
     1441 ccggcttcag gggagccgca tcatagtgcc ccttcggcac gatcacgccc gcgcccgaga
     1501 ggaacgtcaa cgccgcaggg aacggcttcg tcagattgat gcgcactttg aggtcgccgg
     1561 tcttctccac cgacttgatc caggtgaggt agctccgcga gaacgagccg ttggccggat
     1621 cgacgacgaa attcagcgag tagacgacgt cgtcggcatc gagcgccttg ccactgtgga
     1681 acttcacgcc cgcccgcaga tcgaagtcga gcgtgacgtt gtcgacccat ttccagccgg
     1741 tcgccagcag cggctgaggc ttgtacgtct cgtcgagcag cacgaggcga tcgaagatca
     1801 ggaacgagat gatgaagttt tcgcgcgtgt tgaggaagtt cgtatccgca attgggttct
     1861 cgcggtccga cgcccacacc agcgtatcgt tcgccttgcc ggcttcgagc ggaaccgggg
     1921 ccaaggcggc gatacccgcc agcagtcctc cggcgacaag cccgcgcatc ttcagctgca
     1981 tcgtctcctc ccgttctggc tttgcggcag catcgaacat gcccgcgcct aaagcaaggc
     2041 gggaaccgat ggacggcggg gccgaattct cagaccgtgg cgaactcgga ccttgcgtag
     2101 ccctggaggt agaggagcgc ggtcaggtcg ccatggcgga tcgcggcatg cgcctcggcg
     2161 gcaacgatcg gcttggcgcg aaaggcgacg ccgagacccg cttggcggat catggcgagg
     2221 tcgttggcgc cgtcgccgac cgcgagcgtc gctgccggcg cgatgcctcg cgcggaaacg
     2281 agcccctcga gcatctcccg cttcgcctcg cggccgagga tcggctggac gacgcgcccg
     2341 gtcatggtct cgcccgcgag ctcgagcgtg ttcgcgcgat gcatgtcgaa gccgacggcc
     2401 ttggccaccc ggctcgtgaa gaacgtgaag ccgcccgaga cgagcgcggt ataggcgccg
     2461 ttcgcacgca tcgtctggac gagcgtccgc gcgcccggca tcagcgagat gcgccgctcg
     2521 aagacgcgct cgagatcgac cgtagtaacg cccttcaaga gtgcgagccg cgcctcgagc
     2581 gccgcctcga aaggaagctc gcctcgcatc gcccgttcgg tcaccgctgc gacctcgcgc
     2641 ttgcggccga cgacgtcggc catctcgtct atgcactcct gcccgatgat cgtgctgtcc
     2701 atatcggaga tcagcagcgc cttgcgtcgg cctgccgccg ggacgatcgc gatgtcgagt
     2761 cgggcactct ccgcaaacgg ctgcagccgg tcgacgaagc cgcgcgccgc gctcccggcg
     2821 agctcgatgt cgagctcgca ggcctccttc ggcgcgagcc agcgggctgc ggcgatgtcg
     2881 aggcgcggct cgccggcgac aagcgcgcgg atgcgctcct cagtctcttc cccgcccggc
     2941 ggggcaccag tcacgacaac cacgaagcgc atcgccgcaa gccttcagca cacgtagctc
     3001 agtttcgtga agcggtggcg catcgcagtc acgtcgggcg caatggcctc gggcgcctcg
     3061 ttgcccgcaa ggacacgcgc gacgaggcgg gcgagcggag cgcagtcctg cacgtcgaag
     3121 ccccagcgca cgatctctgg cgtgccgatc ctgaggccgt tgttgtcgcc gtccacagcc
     3181 gcgatcggca agccgatgcc acaggcgaga atgttggcgc ggcgcaagag cttcgaggcg
     3241 gcctgtcctc cgccccagcg atgcgcctcg atcgcgaact ggtgcgaggt cgtgaagccg
     3301 cccgccgtcc tgtgcactgg aaggccttcc gcggccagcg cctcggcaag agccttcgcc
     3361 atctcgacca tcttggcggc ataggcgcgc ccatactcgc gccagtcgag cagtgtcacg
     3421 gcgagcgctg ccgccttcga gacatcgaaa ttcgcggtga gccccgggaa cgctatgcgg
     3481 tcgatcctct gcgcgagctt cgcgtcgttg gtgagcacga ggcccgccgg cggcccgccg
     3541 aggctcttgt aggtgctcat cgtcatgaga tgcgcgccct cggcaagcgg ctgctgccag
     3601 gcgcgccccg cgatcagccc cgacatgtgc gccgcatcga acagcaattt cgcgccgacc
     3661 cggtcggcga tcgcgcgcag ctcgcggacg gggtgcggga gcagattgag gctggcgccg
     3721 atggtgatga gcttcggctt cacgcgctcg gcaagctcgg cgagcctggc gacgtcgacc
     3781 gtatagtgcg ccgcgtcgat cggcgccggg tggatcgcga caccgtagag ccctgcggcg
     3841 ccatcgcgat gatgcgtgac atgtccgccg atctccgccg gcggtacgat gacggcgtct
     3901 cccggctggc aggtcgacat gaaggcatag aggttggcga ttgcccctga aggcacgcgg
     3961 atctcggcga atttcgcgcc gaacacctcg gcggcgagtt cggccgtgat cacctcgatc
     4021 tcctcgcaag cctcgagacc catctcgtac ttgtcgcccg cgtagccgag cgagggccgg
     4081 acgccgagac cagaggcgag taacgcctcc gcacgtgggt tcatcgtgtt cgtggccgga
     4141 ttgagattga agcaatcctt ctcgtggatt tcgcgattgc gatctgcgag ggcttcgatc
     4201 cgatgctgga gcgccgccga cccgagcgtt gcggccgacg cagcatagcg cgcctgcacg
     4261 tcctcgacgg ctggcggcac ccaggggcgg cgtgcgagcg tcggcataag atcgtctccg
     4321 tgatcggctt tacatgccga gtagcgcctc gatcgtggcc ggtcaaatgc aaggagggtc
     4381 gcgagctcat ggcagatgcg acacggaacc aggacatcgt ggtcatcaca ggtggatcgg
     4441 aagggatcgg cctcgccttc gcacgcgcct atcacgacgc ggggcggccg ctgctcctga
     4501 tcgcccgcag cgccgagcgg ctcgcagcag cccgcagcgc cctcggggac acacgtgtgc
     4561 acaccctcgc actcgacatc acgcgcgatg acgcgctcga ggcaatcgat gcggcactcg
     4621 ccgggatcgg cgggcagtgc gacgagctcg tcaactcggc cggcttcgga ttgagtggca
     4681 atttcgcctc tctcgcgccc gggcgcctcg aagcgctcgt caccctcaac gtgaccgccg
     4741 ccatgcgtct gatgcgccac gtgctcccgg gcatgatcga gcggcggcgc ggcctgatca
     4801 tcaatgtcgc ttcgctcggt ggcctcgtcc ccgggcccca tcaggcggcc tactacgcga
     4861 gcaaggcgtt cctcatctcg ctcagcgagg ccgtcgccgc cgaagtgaaa gcgcacggcg
     4921 tgcgcgtcat ggctctggcc ccaggaccga tctcgacgga gttccatcgc cggatgggct
     4981 ccgagagcga cctctacctc aagttgctgc ctgtgcagcg tcccgggacg gtggcacgtt
     5041 gggcgcggct cggcgcagcc cttggcgccc gcgtgattgt cccaggcctc cttcagtcga
     5101 tagcagcgct cactttgaga gtgctgccgc atcggctatc aattccgatc atggcgatct
     5161 tgctgaggcc gcgcggccgg gcgcggtcca tgtcgacatc gcattaacct cggtaggtga
     5221 gcaccgcttg cagtttgtta gggaatgggc gctacaaacg ggttgtgcgc cgtggcttca
     5281 agttgcgcgt tcccggcgcc tctatcgcgg cggcgcggag accatgctcc tccatggtct
     5341 tcgcccgccg ttcgccattt gcagcgcggg attgtctgcg tgagtcgcca accggtgcat
     5401 aagcctccgg tcgtgatcgt gacgcttacg ctcgacgtcg gcggcaccga gcaacacatt
     5461 gccaccgtag cacctttgct ggtcgagcgc ggttggcccg tctccgtcta ctgcctcaat
     5521 cgggctggtg agcaggccga gctattgcgc tcgcgcggtg tcgacgtcat cgctcccccg
     5581 cgcgaggccg cgggtcggca gacgccgcgg gcaaagcgcg ctgtgctctc gacgctggca
     5641 gcggccgacc ttttccggaa gtatcgccgc cagaagccgg caatcgccca caccttcctg
     5701 actgagccat tcctgttcgc cggaccggcc ggcatcctcg ctcgcgtgcc gatccgggtc
     5761 atgagccggc gcgagctcaa ggcgacgcga accaactggc cgggagcccg ggccgtcgag
     5821 cgccgcctca tcgcccgcat ggccgccgtg ctcggcaact cacggagcgt cgtcgccgag
     5881 ctcatcgagg acggcgcccc ccgagatcgc atcggcctca tctacaatgg catcgacgtg
     5941 cgccgcttct ccgcaggggt cgaccgggag cgcctccgtc gcgatctcgg catagccggc
     6001 gacggccgca ttgccatcat ggtcgcaaac ctcttcccgt ataagggcca gcgcgagctc
     6061 atcgagggct tcgcctcggt tgcggcgtcg ctgcccgagc cctggacgct cgtcctcgtc
     6121 ggccgcgacg agggcgacag ggcgcatctc gaggcgcgca tcgctgcgct cggcgttggt
     6181 gatcgcgtgc gcctggtcgg ccagcgaaag gatgtgccgt cgttactggc ggcgagcgac
     6241 atcggcattc acgcctcgcg gcaggaaggc ttctcgaatg ccgtcctcga gcagatggcg
     6301 acgggcctgc ccgtgatcgt caccgaggcc ggcggcaata ccgacgccgt cacccacgac
     6361 aagacgggac tgatcgtccc gatcgaggca ccggaggcgc tcgccgccgc catctccacc
     6421 ctcgcgcgcg accccgcccg gcgcacgcgc ctcggtgccg ccgcccgggc acgtatcgaa
     6481 aaggaattca cgctcgcgcg cgtcgtcgat gcctacgagg cgctctacat ggggctcctc
     6541 gccggaaggc gcccggccga aatcgaaggc gtcggcatcg gtcgctgagc ctcttcagtt
     6601 cagccaacgc ctgagtttcc gtcgaagcca gcccggctcg ctcgctgcag gcccggtccc
     6661 gcgcacatgg tcgtgcgcct cgacgagcag ctcgccgatc cgctttgcgt cgtgctcgcc
     6721 cgtactcgag ctcgcgagat cgtcgatgtt gcgcgcccac acacgttcga tcacggcgag
     6781 aaatgggacg gcgcggagct tcgcccccgg cgcgcgggtg agaacgtctc cgagagccgc
     6841 ttccccgctc ggcaccgcgg cgcggcgacc caagtgcacc atcgagacgg ggatcgacac
     6901 gacatcgtag agcgtcgcga ggtcatgcgc cgcgatgcgc tgtccgagaa tcgcgccgcc
     6961 agcgaactgc cggcggaggc tcttgcagga gctgcaccat gtggcgccga actcgacgag
     7021 gacgagccgc ttcctcgccg cggcccctgc cagcgctatc tggagacgct gctgatgatc
     7081 ggcgcagacg ggatagtcga cgctcggcgg ctggccggtg ccggttgggc gcgccgtatc
     7141 agatggaaaa ccaggacagc ccggccccgc cacgaacgcc gccgcagatt tgccgtcgcc
     7201 ggcgatggcc gtcgttgcga caagcgcccc cgtcagcacg aaagcggcga cgagttgtac
     7261 agagaccctt gcggcccgtg cggcgaatat cggcatgagt tcttgccctt gagcgatcgt
     7321 gccgtctcgg cacatctcag gggcatcgaa gcaagaatgt cgccactccg tgcgctactg
     7381 gcaggcctgg cccatcgcca gcagcgcctg cgtcataccg gagagcgagt acacctccgg
     7441 ggccgccgcg cctttctccg ccgtcacctg cacggtcatg cgcgcgccct tcttcatggc
     7501 ctcgatgagc cgcgcttcgg ttgcaacgtc accgacccag gcgcggtccg cgtccgtgaa
     7561 caggcggaac tgctcgcgcc caacggtcac gagagcctcg ctgcccttcc gggcggcgct
     7621 cgcggtcttc agactgaact cggagcgaac gccgtccttc ggccaggcgg taatgtaggc
     7681 aaatgccccg gtgccggctg tcgctgccgc aaagcagatg cgcctcgcgc ctgtcccatg
     7741 agcgtaggcc cgccactcgc ccgacgtcgc gatcacctcg atcgactgag cgaggctagc
     7801 cggcgctgcc agcacggccg cagccatcgt ggcggccata gcgcgtctcg aagcggccaa
     7861 gcagcatctc cttctcggtg aggcagccgt catatgcgcc ggcccgcgcc ctgccaatag
     7921 ggctcacgca acttccgctt gaagatcttc cccgaatcct cgcgcggcag ctcggcgcgg
     7981 agttcgatct tccgcggcac cttgtagtcg gcgaggtggg ctgcaagatg ggcgcggaat
     8041 gcctcgaccg tcagtcccga cccggcgacc ggctcgacaa gcgcgatcag cgcctcgccg
     8101 aactcgtcgt ccgggacccc gaacacagcg caatcgcgca cgcccggcat ggtatgggcc
     8161 gccgcctcga tctcggccgg gtagatgttc acgccgcccg agatcaccat gtcgcgcttc
     8221 cggtcacaca ggaacaggta gccctcggga tcaaggtagc cgacgtcgcc gcaggtgatg
     8281 tgtccgtcgc ggtcgatctc gcggcgcttc tcgggcaggt tgtggtaggt gaaattgttg
     8341 aacggatcga ggcgcgtata gacctcgccg acgccgccag ccggtacgag cgctcctgca
     8401 tcgtcataga tgcgtacgct cgcaccttcg accggccggc cgaccgtgcc ggggcgcgcg
     8461 agccactcct gcgacgacac gatcgtaacc gcgccgctct ccgtggcacc atagaactcg
     8521 ttgatgacgg gtccccacca gtcgatcata cggcgcttca cgtccggggc gcacggtgcc
     8581 gccgcgtgca tgacgaagcg gagcgaggag acatcgtagc gccggcgcac ctcctcgggg
     8641 agcttcagga gacgtacgaa catcgtcggc accatgaagg tgatctcgat gcggtagcgc
     8701 tcgatcgtct ccaggagctc gatcggatcg aagcgcggca tgagcaccat gacacccgcg
     8761 atccgcgcgc cgcgcacggc aaagctgttc ggtgccgaat ggtagagcgg ccccggcagg
     8821 agcgcccgcg atccaggcac catgccatag gtgcgcacgc gcatccgctc gagggccgcc
     8881 tgctgctccg cattcggagg cgcgcggcgg acacctttcg ggttgccggt cgtgcccgag
     8941 gtatagatca tgctctgcgt cggcggcacg ggcggcccgg tccagggctg gctttcgccg
     9001 aggaaggcct cccagccgag cgcacgtggc aggcgccgct cttcgcccat gacataggcc
     9061 gcgcgcacct ccggcggcgg cggcacgtcg atgacggcaa cccgctccgg aacagcctcg
     9121 gcgaccgcag ccagcaagtc agtatgcccg acgaacacct tcgccgcgca atcgcggagg
     9181 atgtagccga cctcctctcc cttgaagtgc cagttgagcg gcaccgcgta ggcgccgagc
     9241 cgcatggcgg caaagctcgc gaccaggaac accgggtcgt tgcgcatcag gatcgcgaca
     9301 cagtcgccct gcccgacgcc gcgcgccgcg aggccgcccg cggccttcgc gacctgctct
     9361 tcgagcagcg cgtggctcat cacgcgtccg ccggacacga cgccggtgac gttccccgga
     9421 acggtgatgc gatcgaggcc acggttggcc gtgctgccgt gctcacccat tgagaccccc
     9481 gggaccctgt caatatgaag cggaccagtt cttgccgaca gtctgtcacg tcggcccgcg
     9541 gtgtggaatg gcgatcgcgc acgctttgcc tgatcacggc cttgcacgcg ccgctcggcc
     9601 cggtagttga gtctcggcca cggctcgcga taatggcccg ggcgaaaacg cagaaggagt
     9661 tcggcatgtc gcctgcgctg tttctcgacc gtgacggcgt cgtcaatgtc gaccgcgact
     9721 atgtctttcg ccgcgaagac ttcgactggc agccgggcat cttcgatctc gcgcgcacgg
     9781 cgcatcgagc cggccttccc atcgtgatcg tgacgaacca gtctggcatc ggccgcggct
     9841 actacagcga ggtcgacttc caggcgctca ccacctggat gcgcacgcgt ttcgaggacg
     9901 aaggcgcgcc aatccgcgcc gtctaccaca gtccgcacca ccccgaagcg gcacgtcccg
     9961 aatatcgcgc cgcagatcac ccctggcgca aaccgcggcc cggcatgctt ctcgccgccc
    10021 gcgacgagca cggcctggat ctcgtcaact caatcatgat cggcgaccga gtgagcgata
    10081 tcgaggcggg cgcggccgcc ggcgtcggca cactcgtcct cgtcagcgag cactcagccg
    10141 aactgagcac ccgaccgacg catcgcttca caaccatcct tgagactgcc acctggttcg
    10201 ccggcgagat ggccttgcgc gatctggcac ttactcgaaa tagatgaact cgtggtcgcc
    10261 ggtgtagccg gtctccttga acacgaaggc ccactcatcg ggcgtatgga aggcacggca
    10321 ggtgagctgc cagtacatga ggttcacttt ctcgcgctcg ttgcgatagc cctcgacgca
    10381 gacgtacctg gcaccgcgcc cgacgcgctc gatctccttg aaggccggcc acagctcctg
    10441 gatgtagagg ttgtgcagcg tgttgatcga caccacgaga tcgaagtgct tgtcgggcca
    10501 gggaagcttg tgcgccggcc ccaccttgca gaacggtttc acgctctcca tcgtgtgctc
    10561 gaccgcatat ttcgagatgt cgatgccggc cacctcaacg ccgggcaccg cctcgcggaa
    10621 atcgtggagc aggaagccct tccccgagcc gacatcgaga atgcgcatgc cgggctttat
    10681 gccgtaggcc gcgaccatgg cgtcggcgac cttgcgccag cgcccgtcgt acctgtagcc
    10741 gccataaccg gtgtcgcggc cgccgtccca ataatcgtag tcgaacttga cggcgagatc
    10801 ggcgacctcg gccttgtcgc gttgggtgac gcgcgcgagg taatcacgct tcgtcgactt
    10861 gtgcacgagg ctcacgaatt ctctgagcgc catggcgcgg gtctcctact tcaggtcgct
    10921 gcgatgtcgt cgagcttggc gcgggcgcgc tcgatctgtc gggccgagcg ctcggccgag
    10981 cccgccaggt cggcgaacgc ccgccggtcg gccccgagcg gcagaaaatc gttcaggatg
    11041 atgcaggccc acttcacgcg gtaggcannn nnnnnnnnnn nnnnnnnnaa cgattcgccg
    11101 agctcgagac gcctggcaag cgccgtaacg aacgcctcat gctgttccgc cggcaccggg
    11161 atctccggct ggcagcagaa gtccgatgcg agcttcgccg gatcatcacg gcccgcgtac
    11221 tcgaagtcga tgaaggtgac gcggccggcg tcactcaacg cattgtggaa gccgaagtcg
    11281 gagggcgaga ggcattgctc gtgatggtcg agcggtcgcg cgagatcgag gctcgcgcga
    11341 acggcaccct gctcgatgcg cgcccggacg cgctgccatg ctggtacgag ccgcttcttg
    11401 acgagttcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnncaga tgctcgccaa gcgagaagca ggcttccgag cccggcgcga
    11521 gcgcggtcac gggcggcgtc ggccggttca gcgcgacgac gaaatccaac gcggcttcca
    11581 cctctggcgc gccgatctcc cccgctgcga gcttgcgccc cggcacgaac gacataagcg
    11641 cggcgccggc ctcgacatcc gtcgcaatcg gctcgggaat ggcacgcaca ccgcgcgacc
    11701 aggcgagtgt cagaaactcc cactcggcct tgcggcggtc gcgcggatcg cggaggtcac
    11761 gaaagtagct cttgaggagc aggctctggc cgtctgccag cgccacgcga tagacgcggt
    11821 tgttcttgcc gccgctcaag gtctccgtgc tgatcggcgc cgcgcgaccc gcacgcctgg
    11881 cgagttcagc agcgagcgcc agcggtgtcg cttcggccgg catctcacgc cgccccgacg
    11941 ccgagcgcgc gtccgatctc tgcccagctc cgatgccgct cgaacgagtg ctgcttccac
    12001 cgcccaccgt cgtagtggcc atccggatcg aacaggatcg cgcgcaggcc cggaggatag
    12061 cccggcatcg ccaggatctc gggaaggtcg tcgatgaaca cctcgcagcc gagggccgcg
    12121 gcgcgcgcaa ccttggcctc cttggtgagc tcaaaatgca cacgtgcgtc atcgatgagc
    12181 ccacccggcc ccgcgagccg ttgcgccacc agaaagccgc gcgccgccgc atgcagatcg
    12241 tagcgcggcc ccgcgaacgg ctcccgcgtc ttgtggctga cgatggctag ctcgtgcccc
    12301 gccgatttca gtgctgccaa tgcagcgaac gcgcccggat agaccgccac gaggtccatg
    12361 cgcgcgccgt acacgtagcc ctgcagctcc gtgaacagcg ggtcctgccc ctccgcccgc
    12421 agataatcgc ggacactcgt cttgtcggac ggcagatcgc gtggaatgag ctcgcgctcg
    12481 agcgctgcgg catggaacac gccgtcgtag caggcgagcg tattgtcgaa atcgatcccg
    12541 atgcgcatgg cgctcaaccg atcacttgag cgacatgtcg atgagcggcc ggccgacctt
    12601 gccggcggcg agatccttca gcgcctggtc ggcctgagcc agagaatatg gctttgaaag
    12661 cagctcgcgc accgggaaac ggcccgaggc gatgagccgg ccgaagcggc cgtagtcgcg
    12721 gtccggcacg ctgtcgccgc cccatgtccc catcagactc ttgccctggt tgaagatgtt
    12781 cggcgagagg gagagcatcg caccgtgctt agcattgccg atgacgacag cgcggccgcc
    12841 ctgcgcgcgc gtcgcgttga tcgcctgatc catcacggac gggatgccgg ttgcctcgac
    12901 cgcgaggtcg acgccgccgg gcgcgatctt cctgatctcg gcgagcacgt cgcccgcagc
    12961 cggatcgatc acatgcgtcg cgccgtacgt tttcgcgaga tcgcgccgcg aaaccgaaag
    13021 gtcgatgccg atcacgggcg tcgcaccggc gaacgcggca gccatgcagg cgttgaggcc
    13081 gatgccgccc gtgccgaaga cggcgaccgc atcgcccgcc tgcgccttca gcacattgaa
    13141 cacggcaccc atgcccgtcg gcgcggcaca ccccaggagg atggcgacgt ccatgccgag
    13201 gcccgccggc accagagtca gccggttctc gctgacgacg gcatggcgtt ggaaggtcgt
    13261 gaccgcgccc gcgttgacct tcttgccgcc ccacgtgtag acgcagccgc ccgcctcgat
    13321 gcccgagccc ttgagccacg agagcaccac cttgtcgccg gccttgacct tggtgaccgc
    13381 gccgcccgtc tcgaccacgg tacctgtccc ctcgtggccg aggcagtgcg ggagccactt
    13441 gtcctcgcct ttgttgccga ccgcctcgtt gacctggctg ccgcaggcgc ccgagtaggc
    13501 gatctcgacc agcacctggc cggtcttgag tgccggtatc tcgagctcgg ccagcaccag
    13561 gggcttcccc agctcgacga ggatggcggc ttcggttttc atgtggtcac gtctctcgat
    13621 gcaaagtgcg tcagcccgtg ctcagccgag cgccttgagc acgcggtcgg cgatctggtg
    13681 gccggtgagg ccgagctcct cgcgcgcata ctcctgctcg cccgacttct tgaagaaggc
    13741 gtccggcgtg ccgaagcgga tgaaggcctt gggccgcacc gcgcgatcga tcatccactc
    13801 gccgaccgcc gagccgaagc cgccgagtag cgaatgctcc tcgatcgtgg cgacgagccg
    13861 gaacttcgcg aaggcctcgg cgaggcaagc ctcgtcgagg ggcttcacgg tgtgaaacga
    13921 gacgacgcgc gccgagagcc ctttcgcctt cagccgatgc ccggcctcga tcgcctcagg
    13981 cagcatggtg ccgcacgaaa ggaggcacac gtcgcgcccc tcctcgatgg tgatcgcctt
    14041 gccgagcgcg aagctttccg gcacgccctt gtgcacgacc ggttccccct tcttgcccat
    14101 gcggatgtag acgggcttcg gctgcttcag cgcggcacgg agcgcgccgc gcacctccca
    14161 ggcgtccccc ggcgcacaga ccaccatgtt cgggatcgcc cgcaggaacg ccatgtcctc
    14221 gcaagcgtga tgcgtcggcc cgaggcctgc gtacgaaagc cccgcaccga ctgcgacgat
    14281 cgtcacgggg agatcgtgat agcaggcgtc ggtgcggatc tgctcgaggc agcgcgaggt
    14341 gatgaacggt gtgatcgtat aggcgaccgg gcggagcccc gacattgcca tgcctgccgc
    14401 gacacccatc atgttctgct cggcgacgcc gcagttgaag aagcgcgatg gatgcgcgtc
    14461 cttgaacttg tcgaagagcc ggttgccgat atcgcccgac agcatgacga cgcgactgtc
    14521 cgagttcccg agcttcgtca gctcgtccgc gaaagcattt ctcatgcgag ccccagctcc
    14581 ttcctggcct tcacgacctc gtcggccgtc ggtgcgcgat agtgccagtt gttgtcgtcc
    14641 tccatgaacg acacgccctt gcccttcacc gtatgcgcga tgagcgcgac gggcttgccc
    14701 gagccgttcg gcaccttctg catgagctcg gcgagcttgc cgacgtcatg tccgtcgacc
    14761 tcgtgggcgt cccagccgaa ggcgcgccac ttgtcggcga ggggggcgag catcagcgtc
    14821 tcgttggaac gcgccgtcgc ctgccacttg ttgtagtcga cgacgacgca gacgttctcg
    14881 agtttctggg cggcggcgaa catggccgcc tcccacaccg agccctcgtt gttctcgccg
    14941 tcggagagga gcgcatagac gcggaacgtc tggcccttga tgcggcccga gagcgccatg
    15001 ccgcaaccga tcggcagccc atgaccaagc gagcccgtcg ccgcctcgac gcctggcaac
    15061 aggttggccg gcggatgctc ggcgagccgc ccgccgtcct tgcagtaggt cgccagttcc
    15121 tcgaccggaa agaacccctt gaatgcgaga gctgcataga gcgccgccgc cgcgtgcccc
    15181 ttcgagagga tgaagcggtc gcgcagctcg tcggtcggcg tcttcggatc aatcgcgagc
    15241 acgtgccaat aggcggccgc caccacgtcg cagcacgaca gcgacgaggc gaggtgcgcc
    15301 gcctgcgccg catggctcat ctcgacgacc ttgcccctga gctcggccgc cttccgcttc
    15361 aatgccgcgg catcgggcag agcttttccg gttggtctcg tcacggatca cccccacggt
    15421 cgaatgtgct cgtcaggatc gcgctcgggc cgctcgatag cgccgtgcga tccgttcacg
    15481 ccttgtgctc gtagcgcgtc ggcagcttgg cgatgtcgcc ctcgaagcgg cgcgcccagg
    15541 cgatcacatc gtcgataccc tgcccgagcg agatggcatc acgccagcca agctcggtcc
    15601 tgagcttgaa actgtcgagc gtataggccg tatccttgcc ggcccgttcg ggaccaatct
    15661 cgacgcagtc ctcgaagcgc ttgccgacac gggcgaggat catctcgacg agcgtgcgga
    15721 tcgagacgag ttcgtagccc gagatgtgat agcaggcgcc gagcgcgccg cgctccgcga
    15781 tcgcctccgt cgccgtcgac acgtcgttca tgtggatgaa gacgcgcacc gacttgccgc
    15841 cgccgtcgag cttcagcttc tgccccccca tggccgcgac gatcgtgcgc ggaatgatgc
    15901 gatagagctg ctggcccggg ccgtagacgt ttgccgcccg tgtgaacagc accggaaact
    15961 ggtagttggc gaagtaggtt ctgaggtgca tgtcgccggc tgcccgtgag gtcgcatacg
    16021 gtgtcgacgg attgaatggc gcgtcctcgc gcacccagcc ctcggtcgag ccgtaagcct
    16081 cgggagtcgt cacatgcaca taacgcgcga gaccctcgta tcggcggagc cgatccgcga
    16141 gccgggcgag cgagacgacg ttcgtcatca tccagtgctc agggaactgc cagctttcac
    16201 ccaccatgct ctgggcggcg aaattgacaa cgtgtgtcgg ccgctcggcc ttgaagagcc
    16261 tgtcgagttt gtcgaggtcg gtgttgaggt caacgcgctc gaagcgcacc ttgcccggtc
    16321 gcttctgcca cttgtagggg agcagagccg gatgcgcctc ctccgagcgg ctcgtagcaa
    16381 tgacgtcggc gccgcgggcg gccagatgat cgcagaacgt ggcacccgaa aacgagttcg
    16441 agccgagcac caggtacttt gctgcgccgc tcatgcgtgc gcctttcgcg cggcgaccgc
    16501 atcgcggcgc tgatagagcc attgcacgat catgtggcag atgatggttt gcgtatcctc
    16561 ggcgatctgc atgtcgtcga tctggaaatg gatcgggacg tcggccatgg ccttcgcctt
    16621 gccgcccgtg aagccgacga cggcatagct cgtcatgccg atgcgccgcg cctcctcgat
    16681 cgccttgagg atgttcatcg agttgcccga gcccgagaag gcgagcagta catcaccttt
    16741 tcgcgcctgg accgcgagct gataggcgaa aatactgtcg tagccttcgt cgttaccgag
    16801 gcaggtgaga atcgccggat tggccgagag cgcgtgcgcc ctgatccccg agcccttcgt
    16861 cttcgacagg gggtacatca gatcgttggc gaggtgattg gcattgccgg cgctgccgcc
    16921 gttgccgcac aggaacacct gccggcccgt ctcccagcaa tcgaggagat cggctgtcag
    16981 ccgctcgacg ggcgcccagt cgaagccgag cagcacgtcc gaaagccgct gcgagtagcc
    17041 gcggaaatgc cgcgcgggcg agtcacccga atgtgtcgtc gcgccgggca agggccgtgc
    17101 cattgcttcg gtccgtgagg tcatttcacg acctccttct gcatccactt gagattgtac
    17161 cagcggtcgt tctccttgag gacgccctgg cggtacatgc cgaccatttc cttgatggcg
    17221 tcctcgaccg cgatcgtctc gcccggacgc aggccgagct tggagggcgc ctgccgggcg
    17281 gcgtgagccg aaatcgggat ttcgctgtca agccggaaac ggccc
//
LOCUS       DAORNH010000063        17313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2488_length_17313_cov_2.663982, whole genome shotgun sequence.
ACCESSION   DAORNH010000063 DAORNH010000000
VERSION     DAORNH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17313
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2488_length_17313_cov_2.663982"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK264_08610"
     CDS             <1..258
                     /locus_tag="PK264_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD75987.1"
                     /translation="TLRYYQPKGADEQTPHDQDEIYIVASGRGSVVSGPAEDRLDKRS
                     FGPGDAIFVPAGHVHRFVDFSQDFATWVVFWGPVGGETTRD"
     gene            268..1218
                     /gene="miaA"
                     /locus_tag="PK264_08615"
     CDS             268..1218
                     /gene="miaA"
                     /locus_tag="PK264_08615"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRD75988.1"
                     /translation="MSPARPRLPDAILIAGPTASGKTALALALAERWPSLIINADSMQ
                     VYRDLRILTARPSVDEEARAPHRLFGHVEGGVAYSAGRYIAEVGEALGEARRRERVAI
                     IVGGTGFYLKALLSGLSPIPDVSREIRKRWRDAVSERGGPALHAVLAERDPVMAARLD
                     PTDGQRIARALEVLEASGRSLREWQAIPGRPVIEAARSVRLVLSSERAWLVARARHRL
                     GTMIRDGALDEVRALDGRGLDRSLPIFGALGMRPLAEHLAGRQSLADAIEATARDTAA
                     YIKRQQTWARGQLADWTPIRIGEGVDPSGAALDAINRYIA"
     gene            1370..3142
                     /locus_tag="PK264_08620"
     CDS             1370..3142
                     /locus_tag="PK264_08620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HRD75989.1"
                     /translation="MPRTMTGANMVIQALIDQGVEVVFGYPGGAVLPIYDEIYQQEKV
                     RHILVRQEGGAVHAAEGYARSTGKVGVVLVTSGPGATNAVTGLTDALMDSIPVVCITG
                     QVPTHLIGNDAFQECDTVGITRPCTKHNWLVKDVNDLARVLHEAFYIAASGRPGPVVI
                     DIPKDVQFATGTYVAPSSSTIAHKSYKPRKQAEPKVIDRMVEMIAAAKRPIFYTGGGV
                     INAGPKASQALRELVRLTGFPITSTLMGLGAYPAADKQWLGMLGMHGTYEANMAMHGC
                     DLMINVGARFDDRITGKVSEFSPGSRKIHVDIDPSSINKNIKVDLPVIGDAGYVLEDL
                     LRAWRLKSPTLDRAAHKAWWKQIDQWRARQSLKYKPSADFIMPQYALQRLAEAVKDRD
                     SYITTDVGQHQMWAAQFLPFNEPNRWMTSGGLGTMGYGLPAAIGTQLAHPKATVACVS
                     GDASILMNIQEMSTAVQYRLPVKIVILNNQYMGMVRQWQQLLHGNRLSESYTEALPDF
                     VKLAEAYGAVGMRCEKPGELDQAYAEMLKVSRPVLFDCRVASLANCFPMIPSGKAHNE
                     MLMGEDVSDEEVGSAIGAEGKTLV"
     gene            3221..3487
                     /locus_tag="PK264_08625"
     CDS             3221..3487
                     /locus_tag="PK264_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRD75990.1"
                     /translation="MERTRMSSKGQVIIPKSIREAKGWGQGDILVAEDRPEGVMLRRA
                     SPFPRTIISQVLGAANYEGPRLSDDDIDQALEKDVAERWGRSVT"
     gene            3484..3876
                     /locus_tag="PK264_08630"
     CDS             3484..3876
                     /locus_tag="PK264_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD75991.1"
                     /translation="MIAVDTSVVVRVIVKDDDKQAARARRLLETRSVLLPVSVVLESE
                     WVLRAAYRLDRQVIAAALRGFCGLDNVTVSEPEVIAAALDLYEQGLDLADAIHLCLSR
                     AADGFATFDRKLARLGTKHNLIPPIKEP"
     gene            3889..4437
                     /gene="ilvN"
                     /locus_tag="PK264_08635"
     CDS             3889..4437
                     /gene="ilvN"
                     /locus_tag="PK264_08635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRD75992.1"
                     /translation="MPDPTSQSHYSAPARIEAVETRTLSVLVDNEPGVLARVIGLFSG
                     RGYNIDSLTVTETEHERHLSRITIVTKGTPMVIDQIKNQLDRMVPVHRVVDLSQEAEA
                     LERELALVKVAGKGDKRIEALRLAEIFRANVIDASVEHFVFEITGRSSKIEQFIAIMT
                     GLGLVEVSRTGIAAIARGPKGL"
     gene            4442..5077
                     /locus_tag="PK264_08640"
     CDS             4442..5077
                     /locus_tag="PK264_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRD75993.1"
                     /translation="MPADLLLGLVVFCWVTGVTPGPNNIMLLSSGLTYGSRATLPHLM
                     GVAVGFAVMVALVGLGIGRLFEAWPALYAILKIAGALYLLYLAWMIATAGAIEADGQA
                     RGRPMTFIEAALFQWVNPKGWVMAVGSVTTYAGIANFPANVALIAAMFGTIGVIASGV
                     WMLFGELMKRWLTDPRRVRAFNIAMALGLVLSLWPVVADVLPEGVKFRKWI"
     gene            5262..7139
                     /locus_tag="PK264_08645"
     CDS             5262..7139
                     /locus_tag="PK264_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRD75994.1"
                     /translation="MRTKETIKAAIAAAEAWVHSRVHPSVVKDKFEGARHAALIGSHI
                     LGGLLAGIVFATFLSWTGAFGIAAILAALWFLSPFLIAGYLSRTGKLDAAHLLSAVNL
                     TGIVVIAAVLSGGGTSFALVWLAIVPLEAALSANRRMMIAATALAVTALIGLHAATVM
                     DLLPAMTALAVPADKLALVSHLAAIAYAGGLASSVQQMHRDSQKEIEAGRARFRLIAE
                     NANDLVTQHDATGRMTFASLASRRLLGLEPDRLIAAGMESQLSVDDRVRYSRAIAQCL
                     ATGAPVAEEFRLHRIASDASLAVEQRFVEMRCQPVFGAESIDGRPMAVIAVTRDISER
                     KANEQALAKARDEAEKASVAKTSFLAAMSHELRTPLSSIIGFAELLHRELLIRSREPK
                     HADYCRIIHESGEHLLSLVKDLLDVAKIESGKYTIIVEPVSIAEIVRSAVETMKPAAD
                     QAEVTIVRSVATDLPDALADRRAVKQILLNLISNACKFTNSGGEISVKAEQRGERIAL
                     IVADTGIGIGPEHIARIGQPFYQIDTPLARRVDGAGLGLSIVRGLVELHGGEMTVSSK
                     VGEGTEFTILFKAELGDEDEAGIAAPRALRDAPAVTTTAPSAAASVHLELQLDSAPET
                     R"
     gene            complement(7146..7751)
                     /locus_tag="PK264_08650"
     CDS             complement(7146..7751)
                     /locus_tag="PK264_08650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRD75995.1"
                     /translation="MVRRRDDLVFMPGKYVFPGGRVDKEDRLIAPYDDLSPDEVRRLM
                     HEMKGYASALRARAIALAAVREAFEEAGLVIGEPVPMEIATWAPFYKLGFVPRLAPLR
                     LFARAITPPGRPRRFDTRFFCVSAAEIAHREAVADGELSGLHWMTFDEARALDVPHIT
                     RVVLEDLTDRMRHGPLSAGLGTVPFYHHKNGTFHRDLIPAA"
     gene            complement(7751..8083)
                     /locus_tag="PK264_08655"
     CDS             complement(7751..8083)
                     /locus_tag="PK264_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRD75996.1"
                     /translation="MAKVDHYGLLGVSKDASPGEVTKAYDAAKAAAKGERLAAIDEAY
                     ATLSDPQKRIFYDQQLYVVSTLPPRKSKSAETSGTPVAARIKPRDAATLVIVDRTTSS
                     PRGRSQRC"
     gene            complement(8107..8508)
                     /locus_tag="PK264_08660"
     CDS             complement(8107..8508)
                     /locus_tag="PK264_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HRD75997.1"
                     /translation="MLRGQAGRCPACGTGRIFTRYLKVADTCNRCGTELHHHRADDAP
                     PYFTILIVGHIIVGGLLALERAAAPPSWVHIAVWVPLTILMSMWLLPRVKGALIALQW
                     ALRMHGFGGPAGATEPHDGYRPNEDAPRSGR"
     gene            8925..9605
                     /gene="nthA"
                     /locus_tag="PK264_08665"
     CDS             8925..9605
                     /gene="nthA"
                     /locus_tag="PK264_08665"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HRD75998.1"
                     /translation="MTAHDHSHDHDHGQGHGHSHDHDHHHDHGSELSPLQLRVRALET
                     VLAEKGYIEPAVLDQIVEAYETRVGPHIGARVIARAWSDPAFKAALIKDASEAITQLG
                     LENKVGDHLIAVENTAGEHNMVVCTLCSCYPWDVLGLPPVWYKSFAYRSRTVKEPRAV
                     LADFGVRLPDAMRIRVWDSTAETRFMVVPMRPAGTEGWSEERLAKLVTRDSMIGTGLA
                     LDPKEVAA"
     gene            9602..10261
                     /gene="nthB"
                     /locus_tag="PK264_08670"
     CDS             9602..10261
                     /gene="nthB"
                     /locus_tag="PK264_08670"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HRD75999.1"
                     /translation="MNGVHDMGGMDGFGKVEPEKNEPVFHGAWEGRVLAMSRAIGATG
                     AWNIDVGRFGIERLPPATYLASSYYERWQRRNELLCIERGLVTAEEIEAGHASGKGKA
                     MPRPPLKSNSIGDMRRGTFYADAQGPQKFKIGDRVRMRNMHPASHTRLPRFVRGHVGV
                     VERINGCHIYPDALVADGKKVGEWLYTVVFDNRTLWGADADPTVAVSVEAFEPYMEPA
                     A"
     gene            10258..10644
                     /locus_tag="PK264_08675"
     CDS             10258..10644
                     /locus_tag="PK264_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HRD76000.1"
                     /translation="MSAVDAKTRAALDGVPGIPCAAEGPTFTEPWQAQAFAMALALQQ
                     KGLFTWGEWAQMIGEEIRKAQAAGDPDTGETYYQHWLATLERMSIAKGLTDPYKLVRH
                     YEAWEHAAERTPHGTPIELKAEDFEQ"
     gene            10726..11106
                     /locus_tag="PK264_08680"
     CDS             10726..11106
                     /locus_tag="PK264_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76001.1"
                     /translation="MRLVRLSMTAALAVMVLTSGLVAQQPPGVMAPVASERMIDVAVT
                     YSFMVPIKGEDLLAQSKSLEAARRMIYEIAANECATMRATIAATCRLERFNVQSNMQR
                     HGPGQEMAQVSANASYKVGLKSGQ"
     gene            11109..11735
                     /locus_tag="PK264_08685"
     CDS             11109..11735
                     /locus_tag="PK264_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76002.1"
                     /translation="MPRRAHDAACRIAAVCGALASVLAFIGEARAERRDAAKFSYCGL
                     GHAEAHSSVQPGGGGDTVQIGGDGDAYVFIYDSASPLTLDDHDRLIIENAVPSALEFK
                     RVGDHLAICNPTSGGEVVIERHYCRSARLAETDVANNEIEEIAFLAAGEIWIADVLYA
                     EYLKDRSAFPRHVARYYGPGRPQDGWRVRPFSEVLPDWVRPADTCGAD"
     gene            complement(11752..11994)
                     /locus_tag="PK264_08690"
     CDS             complement(11752..11994)
                     /locus_tag="PK264_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76003.1"
                     /translation="MLPAVVLIPVLAVGFSDYPVFVNPKAKIEAVLDRGPIQELIVKC
                     PRGTAIIAYSKIERLFCPAKGGCTKSVDEAIGRACR"
     gene            12242..13123
                     /gene="ugpA"
                     /locus_tag="PK264_08695"
     CDS             12242..13123
                     /gene="ugpA"
                     /locus_tag="PK264_08695"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HRD76004.1"
                     /translation="MEKRAIFGGWAFPLLLLAPQLIITLVFFYVPAAQAIWQSFLLQD
                     AFGLKTSFVWFENYQDLAKNHDYFRALGNTAVFSAAVTFFSLSIALLLAVQADKSIKG
                     AAVYKTLLIWPYAVAPAVAGVLWLFMFQPSLGLVARGIKSLGFEWDPLINGRHGMILV
                     ILASVWKQVSYNFLFFLAGLQAIPRSVYEAAAIDGARPARRFWTITFPLLSPTAFFLL
                     VVNIVYVFFDTFGIIDAMTGGGPSGATETLVYKVYFDGRLGGNLGGSAAQSVILMLIV
                     ITLTAIQFKYIERKVTY"
     gene            complement(13273..14034)
                     /locus_tag="PK264_08700"
     CDS             complement(13273..14034)
                     /locus_tag="PK264_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD76005.1"
                     /translation="MRLHIFSDLHADIAAIKPIVFRPDADVAVVAGDVCENMRLGLPL
                     LRRIIPAPMPIVFVAGNHEFYNLHFETHLARGRAIANELGITLLENETAVIAGVRFIG
                     ATLWTDYDLNGIERRARSMEIAAHSLNDHGAIRCGPEERLFTPADARELHLLSRAYLE
                     AELGERHTGPTVVVTHHLPHPGSIHRAYAGHAVNPAFATDLSELIARTEPELWVHGHT
                     HASADHRVGRTRIVCNPHGYRRENPAFDPGLIIEI"
     gene            complement(14065..>15154)
                     /locus_tag="PK264_08705"
     CDS             complement(14065..>15154)
                     /locus_tag="PK264_08705"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD76006.1"
                     /translation="STSHATVARADGDLAALLYTSGTTGRSKGAMITHLNQSSNALVL
                     HKAWRFVPGDVLLHALPTFHTHGLFVALHTAFLNGSAMHWLAKFDLDLALKLLPKSTV
                     MMGVPTFYTRLLGAKEFGREHCRSMRLFVSGSAPLLAETHTEFEARTGHRILERYGMT
                     EAGMITSNPYDPAGRIAGSVGMALPDISVRVAAADGTHMPDGEVGVLEIRGPNLFSGY
                     WRNPEKTKEEFRADGYFITGDLAVKDTTGRVTIVGRAKDLIISGGFNVYPKEIEEEIN
                     ALPGVGESAVVGIPHPDFGEGVIAVLTAAPGANAPSEADVLAALKDRLAKFKLPKRVF
                     VVGELPRNAMGKVQKADLRKQHLELFKI"
     assembly_gap    15155..15213
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15214..>15489)
                     /locus_tag="PK264_08710"
     CDS             complement(<15214..>15489)
                     /locus_tag="PK264_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD76007.1"
                     /translation="YRGLIDEVSRTANALADLGVRPGDRVTAQVEKSIGNILLYLATL
                     KVGATYNPLNTAYTAAELEYFIGDAEPALLVLPKERLDKLAAVARKAG"
     assembly_gap    15490..15545
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15649..17271)
                     /locus_tag="PK264_08715"
     CDS             complement(15649..17271)
                     /locus_tag="PK264_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76008.1"
                     /translation="MTFDYVIIGAGSAGATIANRLTESGRYSVLLLEAGPSDLKSPYI
                     HFPAGFMRLLDDRRVNWVYHTEPEERTGSRKLLYPRGKVLGGSSSINGVLQVRGQHQD
                     FDDWAAMGCRGWSFREVLPFFRRSETYLPGDPAYRGKSGPLIVSDIANHHPLTLDFVK
                     AGQELGLPYNPDYNGAQQEGVGTYQQTRKGRLRMSTARSYLRPAMKRANLKVETEAHV
                     LGLKFEGNRIAGLSYRKGKADGASPPIEALAGREVILCAGAINSPHILQLSGIGAPDD
                     LKRIGVPVRVALPGVGYNFHDHYVARVVRNVRGRVTVNEQSRGWRLGVEVLRYVLKGD
                     GILTYAAGNGVAFVRSEGHMTRPDIQLSFAPASFADGRLGVLADRPGMTCGGWQLRPT
                     SRGSVLAKSSNPAEAPAIRPNYLAEEVDQRAMVAVLRWSRALLNAPVFADCGAEEAVP
                     GPGITTDDEWLAYARANGTTVYHPVGTCKMGTDRLAVVDPELRVHGIAGLRVADASIM
                     PVTTSGNTNAPTIMIGEKAADMILAAAAQAGR"
BASE COUNT         2893 a         5370 c         5965 g         2970 t
ORIGIN      
        1 acgctgcgct actatcagcc gaagggtgcc gatgagcaga cgccgcacga ccaggacgag
       61 atctatatcg tcgcctccgg gcgcggatcg gtcgtttcgg gacctgcgga ggaccggctc
      121 gacaagcggt cgttcggccc gggcgacgcg atcttcgtgc cggcggggca cgtgcaccgc
      181 ttcgtcgatt tctcacagga ttttgcgacc tgggtcgtct tttggggtcc ggtcggcggc
      241 gagacgacgc gcgactgagc ggcactgatg agccccgcgc gtccgcgtct ccccgatgcc
      301 atcctgattg cgggaccgac cgcctcgggg aagacggcgc tggcgctggc gcttgcggag
      361 cgctggccat cgctgatcat caacgccgac agcatgcagg tgtaccgcga cctgcgcata
      421 ctgaccgccc gcccgagcgt ggacgaggag gcgcgggcgc cccatcgact gtttggtcac
      481 gtcgaagggg gcgtggccta ctcggccggt cgctatatcg cggaagtagg agaggcgctc
      541 ggcgaggcgc ggcggcggga gcgggtcgcg atcatcgtcg ggggcacggg cttctatctg
      601 aaggcgcttc tcagcggact ttcgcccatc ccggacgtat cgcgtgagat ccgcaagcgc
      661 tggcgggatg cggtctccga gcggggcggt ccggcactgc atgccgtact cgctgagcgt
      721 gatcccgtga tggcggcgcg gctcgacccg acggatggtc agcgcatcgc ccgggccctc
      781 gaggtgctgg aggcgagcgg gcgctcgctc cgcgagtggc aggcgatccc gggccggccg
      841 gtgatcgagg cggcacggag tgtccggctc gtgctctcaa gcgagcgcgc ctggctcgtc
      901 gcacgggcgc ggcatcggct cggtacgatg atccgggatg gcgctctcga tgaggtgcgc
      961 gcactggatg ggcgcgggtt agaccggagc ctgccgatct tcggggcgct cgggatgcgc
     1021 ccgctcgccg agcatttggc aggccggcag tcgctcgccg atgccatcga ggcgaccgcg
     1081 cgcgatacgg cggcgtacat caagcggcag cagacctggg cgcgggggca actcgccgac
     1141 tggacaccga tcaggattgg cgagggggtt gatcccagcg gggctgccct tgatgcaatt
     1201 aaccgctata ttgcttgata tggcatatat gaataaagtt cattgcgaat ttgagcgtca
     1261 tcagtgattt tttattgcga cagagcgatt cgtgtggtgt cacagcgtct cgcgccggcc
     1321 tggaggccgg cagcaacgag attcgcacgc gaacgtttgg gagagcccga tgccccgcac
     1381 catgaccggc gccaacatgg tcatccaggc cctgatcgat caaggcgtcg aggtcgtctt
     1441 cggctatccg ggcggcgccg tcttgccgat ttatgacgag atctaccagc aggagaaggt
     1501 gcgccacatc ctcgtccgcc aggagggcgg tgcggtgcat gccgctgaag gctatgcgcg
     1561 ctcgacgggt aaggtcggcg tcgtgctggt cacctcgggg ccgggcgcga ccaatgccgt
     1621 gacggggctc acggacgcgc tcatggacag catcccggtc gtgtgcatca cggggcaggt
     1681 gccgacgcac ctcatcggca acgacgcctt ccaggagtgc gatacggtcg gcatcacgcg
     1741 accctgcacg aagcacaact ggctcgtgaa ggacgtgaac gatctggcgc gcgtgctgca
     1801 cgaggcattc tacatcgcgg cctctggccg tccagggccg gtcgtcatcg acatcccgaa
     1861 ggacgtgcag ttcgcgacgg gcacctatgt cgccccgtcg tcttcgacca ttgcgcacaa
     1921 gagctacaag ccgcgcaagc aggccgagcc gaaggtcatc gaccggatgg tcgagatgat
     1981 cgccgcagcc aagcgcccga ttttctacac cggcggcggc gttatcaatg ccgggccgaa
     2041 ggcgagccag gcgttgcgcg agctcgtgcg cctcacgggc ttcccgatca cctcgacgct
     2101 gatggggctc ggcgcctacc cggccgccga caagcaatgg ctcggcatgc tcggcatgca
     2161 cggcacctac gaagcgaaca tggcgatgca cggctgcgat ctgatgatca atgtcggcgc
     2221 ccgcttcgac gatcgcatca cgggtaaggt ctccgagttc tcgcccggat cgcgcaagat
     2281 ccacgtcgac atcgatccct cctcgatcaa caagaacatc aaggtcgatc tccccgtgat
     2341 cggggacgcc ggctacgtgc tcgaggatct cttgagggcg tggcggctca agtcgccgac
     2401 gcttgaccgt gccgcgcaca aggcatggtg gaagcagatc gaccagtggc gtgcgcgaca
     2461 gagcctcaag tacaagccat ctgctgattt catcatgccg caatatgcgc tgcagcggct
     2521 cgccgaggca gtgaaggatc gcgacagcta cattacgacc gatgtcggcc agcaccagat
     2581 gtgggccgcg cagtttctcc cgttcaacga accgaaccgc tggatgacat caggcgggct
     2641 cggcacgatg ggctatgggc ttccggcggc gatcgggaca caactcgcgc atccgaaggc
     2701 gacggtcgcc tgcgtctcgg gcgatgcctc gatcctcatg aacattcagg agatgtcgac
     2761 ggcggtgcag taccggctgc cggtgaagat cgtgatcctc aacaaccagt acatgggcat
     2821 ggtgcgccag tggcagcaac tcctccacgg caaccgattg tcggagagct acacggaggc
     2881 gctcccggat ttcgtgaagc tcgcggaggc ctacggcgct gtcggcatgc gctgcgagaa
     2941 gccgggcgag ctcgatcagg cctatgccga gatgctgaag gtgagccggc ccgtgctgtt
     3001 cgactgccgc gtcgcctcgc tcgccaactg cttcccgatg atcccgtccg gaaaggcgca
     3061 caacgagatg ctgatgggcg aggacgtctc cgacgaggag gtcggcagcg ccatcggtgc
     3121 tgagggcaag accttggtgt gaggcgaggc ttgcgcttcg gaggcaatct tactatttct
     3181 taccgtgggc tgccatgcct ttggcgagga gcatttggcc gtggagcgga cccggatgtc
     3241 gagcaagggg caggtcatta tcccgaagag cattcgggag gcaaaggggt gggggcaagg
     3301 cgatattctc gtcgcggagg atcggcctga aggcgtgatg ctacgccggg ccagtccttt
     3361 tccacgaacg atcatctccc aggtgctcgg ggcagccaac tacgagggcc cgcggttgtc
     3421 ggacgacgac atcgatcagg cgctcgagaa ggacgtcgcg gagcgctggg ggcgctccgt
     3481 cacttgattg ccgtcgatac aagtgtcgtc gtgagggtga tcgtcaagga cgatgacaag
     3541 caggcggcgc gcgcaaggcg gctactcgag acgcgctcag tcctgctgcc agtgtctgtc
     3601 gttctggaat cggagtgggt gctacgcgcg gcctatcggc tcgacaggca ggtgatcgcg
     3661 gcagcgctgc gcgggttctg cgggctcgac aatgtcacag tctctgagcc ggaagtcatc
     3721 gcagcggcgc tcgatctcta cgagcaaggt ctcgatcttg ccgatgctat tcatctctgc
     3781 ctgtcccgcg cagcagacgg gttcgcaacc ttcgaccgca agctcgcccg cctcgggacg
     3841 aaacacaacc tgataccgcc catcaaggaa ccgtagagcg ccactgccat gcccgatccg
     3901 acatcccagt cacactactc ggcccccgcc cgcatagagg ccgtcgagac gcgcacgctc
     3961 tcggttctcg ttgacaacga gccgggtgtg ctcgcccgcg tcatcggcct cttctcgggg
     4021 cgcggctaca acatcgacag cctgacggtg accgaaaccg agcacgagcg ccatctctcg
     4081 cgcatcacga tcgtcaccaa gggcacgccc atggtgatcg accagatcaa gaaccagctc
     4141 gatcgcatgg tgccggtgca ccgcgtcgtc gacctctcgc aggaggccga ggcgctcgag
     4201 cgcgagctcg ccctcgtcaa ggtcgcgggc aagggcgaca agcgcatcga ggcgctgcgg
     4261 ctcgccgaga tcttccgcgc caacgtgatc gacgcctccg tcgagcactt cgtgttcgag
     4321 attacggggc gctcctcgaa gatcgagcag ttcatcgcca tcatgaccgg gcttggcctc
     4381 gtcgaggtct cgcggaccgg catcgccgcc atcgcgcgcg gaccgaaggg cctttgacgc
     4441 aatgccggcc gacctcctgc tcggactcgt cgtcttctgc tgggtgaccg gggtgacgcc
     4501 gggcccgaac aacatcatgc tgctgagttc gggtctcacc tatgggtcgc gggcaacgct
     4561 gccgcacctg atgggggtcg cggtcggctt cgccgtgatg gtcgcgctcg ttgggctcgg
     4621 aatcggcagg ctgttcgagg cctggccggc gctctatgcg atcctaaaga tagcgggagc
     4681 cctatacctc ctctatctcg cctggatgat cgcaaccgcg ggcgcgatcg aagcggacgg
     4741 tcaagcccgt ggtcgcccga tgacgttcat cgaggcggcg cttttccagt gggtcaatcc
     4801 gaaaggctgg gtgatggcag tcgggtccgt caccacatat gccggcattg cgaattttcc
     4861 cgcgaatgtg gcgctcattg cggccatgtt cggcacgatc ggcgtcattg cgagcggtgt
     4921 gtggatgctg ttcggcgagc tgatgaagcg ctggctgacg gatccgcgcc gggtgcgcgc
     4981 gttcaacatc gccatggccc tcgggcttgt gctgtcacta tggccggtcg ttgccgacgt
     5041 acttcctgaa ggcgttaagt tccggaaatg gatctgaatg gatttcaatc tagatgaaga
     5101 gttcatttca cagtccggcg ggattggcgc gcaagccgaa gaatgagagt ttcgaaaact
     5161 tctcatggcg aagcgaaatg gccaattctt tcagtgtcca ttaagcctaa ccggcaaata
     5221 gtgccagcaa ggggcatgtt ggactttgcg tggggcacgt catgcgtacg aaagagacga
     5281 tcaaggcagc aattgcggca gcggaggctt gggtacatag ccgggtgcat ccgagcgtcg
     5341 tcaaggacaa gttcgaaggg gcgcgtcatg cggcgctgat cggttcgcac attctcggtg
     5401 gcctgcttgc gggcatcgta tttgcgacat tcctgtcgtg gaccggggca ttcgggattg
     5461 ccgcaattct cgcggcgctc tggttcctct cgccgttcct gatcgccggg tatttgtcgc
     5521 gcaccggcaa gctcgatgcc gcgcatctgc tctccgccgt caatctcacc ggcatcgtgg
     5581 tgatcgcggc cgtgctgtcg ggcggcggca catcgtttgc gctcgtgtgg ctggcgatcg
     5641 tgccgctcga agcagcgctt tccgcaaacc gacgcatgat gatcgcagca actgcgcttg
     5701 cggtgacggc gctgatcggg cttcacgccg cgacggtgat ggacctgcta ccggccatga
     5761 cggcactcgc ggtgccggca gacaagctcg ctctcgtctc gcatctggcg gcgatcgcct
     5821 acgccggcgg cctcgcctcg agcgtccagc agatgcatcg cgacagccag aaggagattg
     5881 aggctggccg agcgcgcttc cgcctgatcg ccgagaacgc caacgatctc gtcacgcagc
     5941 atgacgcgac gggccgcatg acgttcgctt cgctcgcgag ccgccgcctc ctcggccttg
     6001 agccggatcg cctgatcgcc gccggtatgg agagccagct gtcggtcgat gatcgggtgc
     6061 gttattcacg ggcgatcgca cagtgcctgg cgacaggagc gccggttgcc gaggagtttc
     6121 gcctgcaccg tattgccagc gacgcgtcgc ttgcggtcga acagcgcttt gtggaaatgc
     6181 gctgccagcc tgtgttcggc gcggaatcga tcgacgggcg tccgatggcc gtgattgccg
     6241 tcacccgtga catctcagag cgcaaggcca acgagcaggc gctcgccaag gcgcgcgacg
     6301 aggccgagaa ggcgagcgtc gccaagacgt cgttcctagc agccatgagc cacgaactgc
     6361 gcacgccctt gagctcaatc ataggcttcg ctgagctgct ccatcgcgag ctgctgatcc
     6421 gctcgcgcga gccgaagcat gccgactatt gccggatcat ccacgagagc ggcgagcatc
     6481 tgctgagcct cgtgaaggac ctcctcgacg tcgccaagat cgagagcggc aagtacacga
     6541 tcatcgtcga gccggtgtcg atcgccgaga tcgtgcggag cgccgtcgag acgatgaaac
     6601 ccgcggcgga tcaggccgag gtgacgatcg tgcggagcgt tgccacggac ctccccgatg
     6661 cgctcgctga caggcgggcc gtgaagcaga tcctgctcaa cctcatctcg aatgcctgca
     6721 aattcacgaa ttccggcggc gaaatcagcg tgaaggcgga gcagcgcggc gagcggatcg
     6781 cactcatcgt tgccgacacg ggcatcggca tcggccccga gcacatcgcg cggatcggcc
     6841 agccgttcta ccagatcgac acgccgctcg cccgccgcgt cgacggcgca gggctcggcc
     6901 tgtcgatcgt acgcggcctc gtggagttgc acggcggcga gatgacggtg tcgagcaagg
     6961 tcggcgaggg caccgagttt acgatcctct tcaaggcgga actcggcgac gaggacgaag
     7021 ccggcatcgc ggccccgcgt gcacttcgag acgcgccggc ggtgacgacg acggcaccgt
     7081 ccgccgcggc ttcggttcat ctagagttgc aactcgactc ggcgcctgag acgcggtgag
     7141 gcgccctatg ccgccggaat gagatcgcga tgaaacgtgc cgttcttgtg gtggtagaac
     7201 ggcacggtac cgaggccggc gctcaaggga ccatggcgca tgcggtcggt caggtcctcg
     7261 agcacgacgc gggtgatgtg ggggacatcg agcgcgcgcg cctcgtcgaa ggtcatccag
     7321 tgcaggcctg agagctcgcc gtcggcgacg gcctcgcggt gcgcgatttc ggctgccgag
     7381 acgcagaaga agcgcgtgtc gaagcggcgc ggccgtccgg gcggtgtgat cgcgcgggcg
     7441 aacaggcgta gtggtgccaa ccgcgggacg aagccgagct tgtagaaagg cgcccaggtc
     7501 gcgatctcca tggggaccgg ctcgccgatg acgagccccg cttcctcgaa tgcctcgcgc
     7561 acggcggcga gggcgatcgc ccgtgcacgc agcgcactcg catagccctt catctcgtgc
     7621 atgagccggc gcacctcgtc cggcgagaga tcgtcgtagg gcgcgatcag gcggtcctcc
     7681 ttgtcgacac gaccgcccgg aaagacgtat ttccccggca tgaagacgag atcgtcgcgg
     7741 cggcggacca tcagcacctt tggctccgcc cccgagggga ggacgttgtg cgatcgacga
     7801 tgacgagggt ggcggcatcg cgcggcttga tgcgggcggc gacgggtgtg ccgctggttt
     7861 ccgccgactt cgacttgcgc ggcggcaagg tcgagacaac gtagagctgc tggtcgtaga
     7921 agatgcgctt ctgcgggtcg gagagggtcg cgtaagcctc atcaatcgcg gcgaggcgct
     7981 cgcccttcgc cgcggccttg gccgcgtcat aggccttggt gacctcgccg gggctcgcgt
     8041 ccttggagac gccgaggagg ccgtagtgat cgactttcgc catggtggct cgaatttctc
     8101 cgcgggttag cgcccgctcc gaggcgcatc ctcgttggga cggtagccgt cgtggggctc
     8161 cgtggcaccg gctggccctc cgaatccgtg catgcggagc gcccattgga gcgcgatgag
     8221 ggcgcccttg acgcgaggca gcaaccacat gctcatcagg atggtgagtg gcacccagac
     8281 ggcaatgtgg acccagctcg gcggggcggc agcgcgctcg agcgccagca gtccgccaac
     8341 aatgatatgg ccgacgatca ggatggtgaa gtagggcggc gcgtcgtcag cacgatgatg
     8401 atgcaactct gtgccgcagc ggttgcatgt gtcggcgacc ttcaggtagc gcgtgaaaat
     8461 ccgccccgtg ccacatgcgg ggcagcggcc ggcctggccg cgaagcatcg cgggccatac
     8521 cgggcgcgct tgctcgggtc gtgtcgatgg cgtggcgtcg gacgtgctgc tgatctcgat
     8581 catccagcca cggaccacgg cgtccgatgc ccgtcaaggc gccatgtcgc cgccggtctg
     8641 tagccaaggc tgtgcagccc tcgaaggagc ctccctgcgc caccgggcct cgggcccaga
     8701 ctcgtgccca aatccgtgcc cagactcggg cccagaactg accggggcac ttccctcgat
     8761 gcgcgcctta tgcgcgcgga ccgccccgga tctggcacgc gttgattgca agatcgcggc
     8821 acaagcggcc tctcgtgacc gcgtcaaaca tcgccgccgg gctggcgctc ggggctgtca
     8881 tcgggcaaag tccgcgcatg agcgcgaacc ggatggggca cgcaatgacg gcgcacgacc
     8941 acagccacga ccatgatcac gggcagggcc acggccacag ccatgatcac gaccaccacc
     9001 acgaccatgg ctcggaactc tcgccgctgc agctgcgcgt gcgggcgctc gaaaccgtgc
     9061 tcgccgagaa gggctacatc gagcccgcgg tgctggacca gatcgtcgag gcctacgaga
     9121 cgcgtgtcgg cccgcacatc ggcgcgcgcg tgatcgccag ggcgtggtct gatccggcct
     9181 tcaaggcggc gctcatcaag gacgcgagcg aggctatcac gcagctcggc ctcgagaaca
     9241 aggtcggcga ccacctgatt gcggtcgaga acacagccgg agagcacaac atggtcgtgt
     9301 gcacgctctg ctcctgctac ccctgggacg tgcttggtct gccgccggtc tggtacaagt
     9361 cgttcgccta tcgttcgcgc acggtgaagg agccgcgcgc ggtgctggcc gacttcggcg
     9421 tgaggctgcc tgacgcgatg cgtatccggg tgtgggattc gacggcagaa acgcgcttca
     9481 tggtggtgcc gatgcggccc gccggcaccg aagggtggag cgaggagcgg ctcgccaagc
     9541 tcgtcacgcg tgacagcatg atcggcaccg gcctcgcact cgatcccaag gaggtcgcgg
     9601 catgaatggc gtgcacgaca tgggcggcat ggacggcttc ggcaaggtcg agcccgagaa
     9661 gaatgagccc gtcttccacg gcgcgtggga gggccgcgtg ctcgccatga gccgggcgat
     9721 cggcgcgacg ggcgcctgga acatcgacgt cggccgcttc ggcatcgagc ggctgccacc
     9781 ggcgacctat ctcgcctcgt cctactatga gcgttggcag cggcggaacg agcttctgtg
     9841 catcgagcgc ggccttgtca cggcggagga gatcgaggca ggccacgcga gtgggaaggg
     9901 caaggcgatg ccgcgtccgc cgctgaagtc aaattccatc ggggacatgc gccgcggcac
     9961 cttctatgca gacgcgcagg gtccgcagaa attcaagatc ggcgaccgcg tgcgcatgcg
    10021 gaacatgcat cccgcctcac atacgcgcct gccgcgcttc gtgcgcgggc acgtcggcgt
    10081 ggtcgagcgc atcaacggct gtcacatcta cccggatgcg ctggtcgcgg acgggaagaa
    10141 ggtcggcgag tggctctaca ccgtcgtctt cgacaatcgc acgctgtggg gggcggatgc
    10201 cgacccgact gtggcggtgt cggtggaagc attcgagccc tacatggagc ccgcggcatg
    10261 agcgcggtcg acgcgaagac gcgggcggcc ctcgacggcg tgccgggcat cccgtgcgcg
    10321 gcggaaggcc cgacgttcac cgagccctgg caggctcagg cctttgccat ggcgctggcg
    10381 ctgcaacaga agggcctgtt cacctggggc gaatgggcgc agatgatcgg cgaggagatc
    10441 aggaaggcgc aggccgcggg cgaccccgat acgggcgaga cctactacca gcactggctc
    10501 gcgactctcg agcggatgtc gatcgccaaa ggcttgaccg acccctacaa gctcgtccgc
    10561 cactacgagg cctgggagca cgcggctgag cgcacgccgc acggcacgcc gatcgagctg
    10621 aaggcagagg acttcgagca gtaggtgggc gcgcgcggcg tcgtaaatgc tccttaaatg
    10681 tcagtatgct ctcctttgcg gtgctcgcgc ttgtaggaga gttcgatgcg tcttgtccgc
    10741 ctttcgatga cggccgccct ggccgtgatg gtgctcacga gcgggctcgt cgcccagcag
    10801 cctccgggcg tgatggcgcc cgtcgccagc gagcgtatga tcgacgtcgc cgtcacctac
    10861 agtttcatgg tgccgatcaa gggcgaggac ctgctcgcgc agagcaagtc gctcgaggcc
    10921 gcgcggcgca tgatctacga gattgccgcc aacgagtgcg ccactatgcg ggcgacgatc
    10981 gcggcgacct gccggctcga gcgcttcaac gtgcagagca acatgcagcg ccatggcccg
    11041 ggccaggaga tggcgcaggt gagtgcgaat gcgtcctaca aggtcggcct caagtcggga
    11101 caatgagcgt gccgaggaga gcgcacgatg cggcttgccg tatcgcggcc gtgtgcggcg
    11161 cgctcgcctc agtgctcgcc ttcatcggcg aggcgcgtgc cgaacgccgt gacgcggcga
    11221 agttcagcta ttgcggcctc ggccacgcgg aggcgcactc gtcggtgcag cccggcggcg
    11281 gcggcgatac ggtccagatc ggcggcgacg gcgacgccta cgtcttcatc tacgacagcg
    11341 cgagccctct cactctcgac gatcatgatc ggctgatcat cgagaacgcg gtgccctcag
    11401 cgctggagtt caaacgcgtc ggggatcacc tcgcgatctg caatccgacg agcggcggcg
    11461 aagtcgtgat cgagcggcat tactgccggt ccgcaaggct cgccgagacg gacgtcgcca
    11521 acaacgagat cgaggagatc gcgttcctgg cggcgggcga gatctggatc gccgatgtgc
    11581 tctacgcgga gtacctgaag gaccggagcg cgtttccgcg ccacgtcgcg agatactatg
    11641 ggccgggccg cccgcaggat ggctggcgcg tgcgtccgtt cagcgaggtc ctgccggact
    11701 gggtaaggcc agcggacacg tgtggcgccg actgagcttg tctcgccgcg ctcagcggca
    11761 ggcccgcccg atcgcctcgt cgacgctctt cgtgcagccg cccttcgccg ggcagaacag
    11821 gcgctcgatc ttcgaatagg cgatgatcgc ggtgccgcgc gggcatttca cgatcagctc
    11881 ctggatgggg ccgcgatcga gcacggcttc gattttggcc ttcggattca cgaacacggg
    11941 gtagtcactg aagccgacgg cgaggaccgg gatcagcaca acggcaggca gcatgggacg
    12001 cctccacatt tcccgcggcc gactgtcgcc gctgttgaac tcacgaaatg ttggattgca
    12061 atgggcgtgc cagaattgcg ttcccggtgg tgcacctcgt tggggtgtca gacgaacgat
    12121 catgacggtc gtgagagtcg atcgcggcgc gaggctgttc cgcgagcacg cgcgcgtgct
    12181 agggttggcc gcacgtccgg gagtgctcag acgagatcgg gcgggcgagc gcggggggcg
    12241 catggagaag cgggcgatct ttggcgggtg ggcttttccg ctgctgctgc ttgcgccgca
    12301 gctcatcatc acgctcgtct tcttctacgt gccggccgcg caggcgatct ggcaatcgtt
    12361 cctgctgcag gatgccttcg gccttaagac ctcgttcgtc tggttcgaga actatcagga
    12421 cctcgcgaag aaccacgatt atttccgggc actcggcaac acggcggtgt tctcggcagc
    12481 agtgacgttc ttctcgctgt cgatcgcgct cctgctcgcg gttcaggcgg acaagtcgat
    12541 caagggtgcg gccgtctaca agacgctgct catttggccg tacgccgtgg ctccggccgt
    12601 cgcgggcgtg ctctggctct ttatgtttca gccctcgctc gggctcgtcg cgcgtggcat
    12661 caagagcctc ggcttcgagt gggaccctct gatcaacggt cgccacggca tgatcctcgt
    12721 gatcctggcg tcggtgtgga agcaggtgag ctacaacttc ctgttcttcc tcgcaggcct
    12781 ccaggcgatc ccgcgctcgg tctacgaggc agccgcgatc gacggcgcgc ggcccgcgcg
    12841 acgcttctgg acgatcacct tcccgctgct ctcaccgacg gccttcttcc tgctcgtcgt
    12901 caacatcgtc tacgtgttct tcgacacctt cggcatcatc gatgcgatga ccggcggcgg
    12961 gccctcggga gcgaccgaga cgctcgtcta caaggtctat ttcgacggtc ggctcggtgg
    13021 caatctcggc ggctcggcgg cgcagtcggt gatcctgatg ctgatcgtga tcacgctgac
    13081 cgcaatccag ttcaagtaca tcgagcggaa ggtgacgtat tgaattgtga gccgctcagg
    13141 tcgccaaggc tacaaaaggc ggctggtctg gcgggtgtcc cgccagtggg agcgcgacgc
    13201 gttgaaggag cgcttccggc acgacgacgg accctcgttc gcgtagcgga tcgcgacacg
    13261 atcggctgct gctcagatct cgatgatgag gccgggatcg aacgccggat tctcgcggcg
    13321 gtaaccgtgg gggttgcaca cgatgcgagt ccggccgaca cggtggtcgg cgctcgcgtg
    13381 cgtgtggccg tgcacccaca gctccggctc cgtgcgcgcg atgagttctg agagatcggt
    13441 cgcgaaggcg gggttcacgg cgtggccggc ataggcgcgg tggatgctgc cgggatgcgg
    13501 cagatgatgc gtgacgacga cggtcgggcc ggtgtgccgc tcgccgagct cggcctcgag
    13561 ataggcgcgt gagaggaggt gcagctcgcg cgcgtcggcc ggtgtaaata aacgctcttc
    13621 cggcccgcag cgtatcgccc cgtgatcatt gagcgagtgg gcggcgatct ccatcgaccg
    13681 cgcgcggcgc tcgatgccgt tcaagtcgta gtcggtccag agcgtcgcgc cgatgaagcg
    13741 cacgccggcg atcactgccg tctcgttctc gagtagcgtg atgccaagtt catttgcgat
    13801 cgcgcggccg cgagcgagat gggtctcgaa gtgcagattg tagaactcgt ggtttcccgc
    13861 gacgaaaacg atcggcatcg gcgctgggat gatccggcgc agcagcggca ggccgagcct
    13921 catgttctcg cagacgtcgc ccgcgaccac ggcaacgtcc gcgtccgggc ggaacacgat
    13981 cggtttgatc gcggcgatat ccgcgtgcag gtccgagaaa atgtggaggc gcatgtgccg
    14041 caactggccg aggacggggc cgggtcagat cttgaagagc tcgaggtgct gtttccggag
    14101 gtcggccttc tgcaccttcc ccatggcatt gcgcggcaac tcaccgacga cgaacacgcg
    14161 cttcggcagc ttgaacttgg cgaggcggtc cttgagtgcc gcgaggacat cggcctcgct
    14221 cggcgcattc gcgccggggg cggcggtcag cacggcgatg acgccttcgc cgaaatccgg
    14281 gtgcgggatg ccgacgaccg cgctctcacc gacgccggga agcgcgttga tttcctcctc
    14341 gatctccttc gggtaaacat tgaagccgcc cgagatgatg aggtccttgg cgcgcccgac
    14401 gatagtgacg cgacccgtcg tgtccttcac ggcgaggtcg cccgtgatga agtagccgtc
    14461 ggcgcggaat tcctccttgg tcttctcggg gttgcgccag tagccggaaa agaggttcgg
    14521 gcccctgatc tcgaggacgc cgacctcgcc gtcgggcatg tgagtgccgt ccgccgcggc
    14581 aacgcgaacg gagatatcgg ggagcgccat gccgactgat ccggcaatgc ggccggcggg
    14641 atcatagggg ttcgaggtga tcatgccggc ttcggtcatg ccatagcgct cgaggatgcg
    14701 gtggccagtg cgggcctcga actcggtgtg cgtctcggcg agcagcggcg ccgagccgga
    14761 cacgaagagg cgcatcgacc tgcaatgctc gcggccgaat tccttggcgc cgagcaagcg
    14821 cgtgtagaac gtcggcacac ccatcatgac ggttgatttc ggcaggagct tcagcgcgag
    14881 atcgaggtcg aacttggcga gccagtgcat cgccgagccg ttgaggaagg ccgtgtgcag
    14941 cgcgacgaag aggccgtgcg tgtggaaggt cggcagtgca tgcagcagta cgtcgcccgg
    15001 gacgaagcgc cacgccttgt ggaggacgag cgcgttcgac gactggttga ggtgcgtgat
    15061 catggcgccc ttggagcgcc cggtcgtgcc cgatgtgtac aggagtgccg cgaggtcccc
    15121 gtcggcccgc gcgactgtcg cgtgcgaggt tgacnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctgcct tcctggcgac ggctgcaagc
    15241 ttgtcgagcc gctccttcgg cagcacgagg agggccggct cggcatcgcc gatgaaatac
    15301 tcgagctcgg ccgcggtata ggccgtgttg agcggattgt aggtcgcgcc gaccttcagc
    15361 gtcgcgagat agaggaggat gttgccgatc gacttctcga cctgggcggt gacgcgatca
    15421 cccggcctca cgccgagatc agcgagagcg ttggcggtgc gggacacctc gtcgatgagt
    15481 ccgcgatagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnngaagc tcgcctcgag taggctgtag agattgcgat cggtcgccat ggttcagtcc
    15601 gttcggagtt aggggtgctc aggcattaca ggcgcaggtc ggcgcgggtc aacggccggc
    15661 ttgggcggca gcggcgagga tcatgtcggc ggccttctcg ccgatcatga tggtcggcgc
    15721 gttggtgttg cccgacgtcg tgaccggcat gatggaggcg tcggcaactc tgaggcccgc
    15781 gatgccgtgg acgcgcagct ccggatcgac gacggccaag cggtccgtgc ccatcttgca
    15841 ggtgccgacg gggtggtaga cggtggtgcc attggcgcgg gcgtaggcga gccattcgtc
    15901 gtcggtcgtg atgccgggac cgggtacggc ttcctcagcg ccgcagtccg cgaacacggg
    15961 cgcattgagg agcgcccggc tccatctgag cacggcgacc atggcgcgct ggtcgacctc
    16021 ctcggcgagg tagttcgggc ggatggccgg agcctcggcc gggttcgacg atttcgcgag
    16081 cacgcttcca cgcgaggtcg gacggagctg ccagccgccg caggtcatgc caggccggtc
    16141 ggcgagcacg ccgaggcggc cgtcggcgaa gcttgccggc gcgaacgaga gctggatgtc
    16201 ggggcgcgtc atgtgacctt ccgagcggac gaaggcaacg ccgttgccgg cagcgtacgt
    16261 gaggatgccg tcgcccttga ggacgtagcg cagcacctcg acgccgagcc gccagccgcg
    16321 cgactgctcg ttgacggtga cgcggccgcg cacattgcgc acgacgcgag ccacgtagtg
    16381 atcgtggaaa ttgtagccga ctcccggcag cgccacgcgc acgggaacgc cgatgcgctt
    16441 caggtcgtcc ggcgcgccga tgccggaaag ctggaggatg tgcggggaat tgatcgcgcc
    16501 ggcgcagagg atcacctcgc gtccggccag cgcctcgatc ggcgggctcg cgccgtcggc
    16561 cttgcccttt cgataggaga gcccggcgat gcggttcccc tcgaacttga gcccgagcac
    16621 gtgcgcctcg gtctcgacct tgagattggc gcgcttcatc gcagggcgga ggtagctccg
    16681 cgcggtgctc atgcgcaacc ggcccttgcg cgtctgctgg taggtgccga cgccctcctg
    16741 ctgcgcgccg ttgtagtcgg gattgtaggg gaggccgagc tcttgtcctg ccttcacgaa
    16801 atcgagcgtc agcgggtggt ggttcgcgat atcggagacg atgagcggcc cggacttgcc
    16861 gcgataggcg ggatcgcccg gaaggtaggt ttcggaacgc ctgaagaagg gcagcacctc
    16921 gcggaagctc cagccgcggc aacccatcgc tgcccagtcg tcgaaatcct ggtgctggcc
    16981 gcgcacctgg aggacgccgt tgatcgagct cgagccgccg agcaccttgc cgcgcgggta
    17041 gaggagcttg cgcgagcccg tccgctcctc aggctcagtg tgatagaccc agttgacgcg
    17101 ccggtcatcg aggaggcgca tgaaccccgc gggaaagtgg atgtagggcg acttgaggtc
    17161 ggaggggccg gcttcgagca gcagcacgga gtagcggccg gattcggtca gtcgattggc
    17221 gatggtcgcg ccggccgaac ccgcgccgat gatgacgtag tcgaaggtca tgccgggtca
    17281 atcctgtggc gaggcctact tcttgacggt gag
//
LOCUS       DAORNH010000064        17308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2490_length_17308_cov_2.670840, whole genome shotgun sequence.
ACCESSION   DAORNH010000064 DAORNH010000000
VERSION     DAORNH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2490_length_17308_cov_2.670840"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK264_08720"
     CDS             complement(<1..526)
                     /locus_tag="PK264_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD76009.1"
                     /translation="MIKLNGIGLKYGRGIEILRGVTFHLQPGSFHFLTGASGAGKTSL
                     LRLLFMSQQPTSGRVELFGQEVSAVTPARRAQLRRRIGIVFQDFRLLDHMTTWENVAL
                     PLMVVGKRIADYKEDVTDLLQWVGLGDRMHAYPSVLSGGEKQRAAIARAVIGKPELLL
                     ADEPTSSLDPKTSVE"
     gene            760..1515
                     /locus_tag="PK264_08725"
     CDS             760..1515
                     /locus_tag="PK264_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRD76010.1"
                     /translation="MTAADTSGDIVTAALLVIGDEILSGRTKDKNIGYIADHCTQIGI
                     QLREVRVVADDEGEIVAAINALRAKFTYLFTTGGIGPTHDDITADCVAKAFGVKIDHD
                     QRALDLLVPYFKKRGVEVTPARLRMARIPDGGELVHNSISVAPGFMIGNVIVMAGIPS
                     VMQVMLDDATKRIRTGRKMLSASMDLNRPEGEIAELFEAHQKLYPDVPMGSYPFFRDN
                     KPGTQLVLRSLDATRLAEAEAGLREKLAAKGWI"
     gene            1531..2175
                     /locus_tag="PK264_08730"
     CDS             1531..2175
                     /locus_tag="PK264_08730"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD76011.1"
                     /translation="MRQTISTIVFDVGETLIDETSMWAGWARYFGVTLEAFLAELDLS
                     IAEGRHHHTVYRRFRPDFDLKEARRERTARGERDIFSAADLYPDALPALAGLRKGGFR
                     IGIAGNQPRASEWALREAGFEADFVASSEAWGVEKPSPAFFAKVAEAAGEPPGHIAYV
                     GDRLDNDVLPARAAGMTAIFLVRGPWGRAHARRADAALAHCTIHGLAEIAALVR"
     gene            2385..3500
                     /locus_tag="PK264_08735"
     CDS             2385..3500
                     /locus_tag="PK264_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76012.1"
                     /translation="MTHVCADASCRHDLDFHASDDRPSRRRALILGGSAAAIGGGLVT
                     ATTGASAQVGDQSSWRFCNKCFTLFFDGYPTKGSCPAGGGHVAQGYIFNLHHDVRKQP
                     PRVTGRFAWQYDWRFCNKCFAMFFDGYPAKGRCPAGGGHVAQGFNFGLDFTNESQRRP
                     PVGVFQTTWRFCGKCNGMFFDGYPTKGRCQGGGAHAAAGWMFHLAFTAGPATPPPPPP
                     GTGAGLGNAIVAFARARLNQCVDTRLQTRPQACPALAPGAVGDGECTHLVQAALASVG
                     ARAPTFQPGNYVWGNVITGGYQPGDIIQFYNARFNGPNSWWQTATQHTAIIEVMRSAT
                     AVTLLHQNSPIRAVTRQDLDLAWPHTGRFVVFRAVRA"
     gene            complement(3515..4684)
                     /gene="mepA"
                     /locus_tag="PK264_08740"
     CDS             complement(3515..4684)
                     /gene="mepA"
                     /locus_tag="PK264_08740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HRD76013.1"
                     /translation="MRRWLLAGLTMVAIVLLPGGISLYVRAQSVATPDIPPPPVADPA
                     AATTAPKAPVVVPPVAKAAPKAAAPKAAPASPQPKAAASPKPVAPAKPAAAAAAPAKA
                     ATPLPKPVPAKELFGHQTAPANLAARAIGSYAKGCLAGAVPLAIDGPAWQAMRLSRNR
                     FWGHPLLVSLVEKLAKEAQAHDGWPGLLVGDISQPRGGPMLTGHASHQIGLDADIWLM
                     PMPPRRLEKKEREEMSAISVLRADNLAVDPTKWTEAHVKLIRRAASYPEVERVLVHPA
                     IKKAMCDGVPADPARAAWLKKVRPIWGHHYHFHIRMGCPAENPTCRAQFAVAPPPTDA
                     DGCGKELADWMKLLSRPPPPPLPPGTPAKPPPPPPPPLTLDQLPTECKIVLEAGR"
     gene            complement(4807..5544)
                     /locus_tag="PK264_08745"
     CDS             complement(4807..5544)
                     /locus_tag="PK264_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76014.1"
                     /translation="MSTDRLSAIAAKLVAARTAGARITLVDAPKTFEEGFAVQDKVVA
                     ALGSPVMGWKVIEMPNGGPVIYAPLLESGRVPAGGTWSVKGSEPAGIELEIAFRMARD
                     VPKGATGSSVLDCVGAGHVVFELCQSRNANPDSVPRHVAVADCILNSGIMVGPEIANW
                     RKQEHKSRPGRLFVDGKVHIEGKSVDPFRALEVLAPALAAHGKQLKAGQIVITGSLIG
                     MNWLHGSHDIRGVIDGFGEVQGRVARA"
     gene            5721..6686
                     /gene="trxB"
                     /locus_tag="PK264_08750"
     CDS             5721..6686
                     /gene="trxB"
                     /locus_tag="PK264_08750"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRD76015.1"
                     /translation="MGGARHHRLVILGSGPAGYTAAIYAARAMLKPLVIQGIQPGGQL
                     TITTDVENYPGFAKVIQGPWLMEEMKAQAEHVGTEIVMDQVVKVELASRPFRLTGDSG
                     DVWTCDALIICTGAQARWMGTPSEEAYKGFGVSACATCDGFFYKGREVIVVGGGNSAV
                     EEALFLTKFASKVTVVHRRDSFRAEKILQERLFKNPKVEVVWNSVVEEIKGPTDPRTV
                     SGVRLKDVKTGKVTDRKVDGVFIAIGHAPATELFKGQLEMKPSGYLITRPDSTATSIP
                     GVFAAGDVKDDVFRQAVTAAGMGCMAALEAEKFLAGHGAAAHAAE"
     gene            6731..7621
                     /locus_tag="PK264_08755"
     CDS             6731..7621
                     /locus_tag="PK264_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD76016.1"
                     /translation="MDWDKLRIFHAAAEAGSFTHAGDSLRMSQSAVSRQVSALEKDLG
                     LALFHRHARGLVLTEQGEVLFRTASEVMTKLQTAETLLSDATTKPAGDLRITATVSLG
                     SVWVTQRIREFLDLYPEIRIELVLNDEPVDLSMRVADVAIWAREPSQSDLIRRPLFHM
                     QIHAYASGTYIRKFGMPKTVSDLDQHRLVSYIGNPPQHLAAINTLESIGRESQSPRVP
                     AFRASTVLALKYAIRAGLGIGLIPDYLTEEEGDLVPVLQELAAPKLPVYFVYPEELKT
                     AKKVQVFRDFLVSKGRQWKF"
     gene            complement(7625..8494)
                     /gene="folD"
                     /locus_tag="PK264_08760"
     CDS             complement(7625..8494)
                     /gene="folD"
                     /locus_tag="PK264_08760"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRD76017.1"
                     /translation="MAEARIIDGKVEAARIRAEVKTVVGDIVARGGRAPGLAVVLVGS
                     DPASEVYVRNKGRQTLEAGMLSFEHKLAATTSEADLLALVARLNAEPAVDGILVQLPL
                     PAHIDARKVIAAISPGKDVDGFHPVNVGRLAIGEPALVPCTPLGSVILAKTVAPSLAG
                     MEAVVIGRSTIVGKPVAQLLLNENATVTIAHSRTRDLSSVVRRADLVIAAVGKPEMVR
                     GDWLKPGAIVIDVGINRIAGADGRDRLVGDVAFEEARRVAGAITPVPGGVGPMTIACL
                     LRNTLEAAGRSIM"
     gene            complement(8512..8838)
                     /locus_tag="PK264_08765"
     CDS             complement(8512..8838)
                     /locus_tag="PK264_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRD76018.1"
                     /translation="MAAGGELYRAATGRLIIRVRLTPKSSADRIEGVEMTAEGPALKA
                     RVRAAPQDGEANAALERLIADWLDVPRRTVEVERGGKSRVKTVAVTGDAAALAPRVGE
                     RLKSQS"
     gene            complement(8841..9128)
                     /locus_tag="PK264_08770"
     CDS             complement(8841..9128)
                     /locus_tag="PK264_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HRD76019.1"
                     /translation="MIEVLPFVHYIIQLYIYVIIASAVFSWLVVFKVVNPHNQMVRSF
                     WQMLVAVTEPLLGPIRRLLPDLGGIDISPVILIVILMVIQSWLLPTIAGML"
     gene            9325..10221
                     /locus_tag="PK264_08775"
     CDS             9325..10221
                     /locus_tag="PK264_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76020.1"
                     /translation="MIWRWLSRAALIGAIAAHPRLRQTVLLAGGVVAFGLAYWFLSSF
                     FATLDQIANPWLQLAFTIATALLVALASVAALVILGAWSRVKVHPAARSPSRERLDEL
                     FAKHGIEAPSITVMPAGRAADGPAVAVIGSPGVGRTTLIRALSEELASRERGANPRFV
                     ELPPLEADPIRQGDAVVMSADFDQVVLIVADDLKAHEMAALETIAKAGKRAIVVLAKA
                     DALRPGERAELVAALSERVAALPGPPVVVAASADPAPVVAELVTAEGTVKTTERRPPR
                     SMAPLADAIEAALTRRGQARLP"
     gene            10218..>10814
                     /locus_tag="PK264_08780"
     CDS             10218..>10814
                     /locus_tag="PK264_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRD76021.1"
                     /translation="MTRDRSFTDALDALRRIADSPELRPGERKAVAQELRDVEAMRDK
                     LAEGRVEITAFGEINAGKSALLNALLGREVFKVAARGGETKVRSAEEWRPDVRELSGL
                     GTKLVVVDTPGLNEVDGAHRARIAERTVRQSDIVLFVVRGDLNDVEISALRELSALHK
                     PLILVLNQIDRFRKSELVEALAAIKSRVAGLVADRDIVF"
     assembly_gap    10815..10928
                     /estimated_length=114
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10929..11514
                     /locus_tag="PK264_08785"
     CDS             <10929..11514
                     /locus_tag="PK264_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HRD76022.1"
                     /translation="LAREGKAVAALNASLFAADVSQRIARLKADARQAEAEAIIERYM
                     LVKASAVALNPVPVLDIIGSMGSDAVMLKHLASVYGQEITTANAGKLAQDIMGAWGIA
                     IAVQWGTQTLLSLMKLKTFGLSTIITAVPQGLAAAWTTHVVGKSAHVYFREGGWGAGG
                     ARQVIKDVLASSSPGSILEPLKARLEARLRGRSA"
     gene            complement(11525..12145)
                     /locus_tag="PK264_08790"
     CDS             complement(11525..12145)
                     /locus_tag="PK264_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRD76023.1"
                     /translation="MSSPRIFLHKNDLPAGLSFGGSAAIDTETMGLLPHRDRLCLVQL
                     SAGDGTAHLVQFDGRDWSAPRLKALLGDPAVLKILHFARFDLGVIERYLGLSMSPIYC
                     TKVASKLARTYTDRHGLKDLVGEVLGIDLSKQQQSSDWGAASLSQQQLAYAASDVLHL
                     HALKSRLDDMLAREGRTALAEATFRYLPVRARLDLAGFAEIDLLAH"
     gene            complement(12339..13208)
                     /locus_tag="PK264_08795"
     CDS             complement(12339..13208)
                     /locus_tag="PK264_08795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HRD76024.1"
                     /translation="MSFHDPGWKIADLSRRQVLATGAATGIVTGFAVAVQPVVAQTMI
                     VTDATGLDAGMIEVKSGTDAIAAYRAAPAGAKGRPVILVVQEIFGVHEHIKDVCRRLA
                     KLGYCAIAPELYQRQGDVAKLKSIDEIRPIVAKVPDAQVMADLDAAVAWAKAAGVGDD
                     SKLGVTGFCWGGRITWLYSAHNPKVKAGVAWYGRLVGDRNANNPTYPVDVAAKLNGPV
                     LGLYGGADAGIPVTTVEEMRKAFGSNAKSQIHVYEGMPHAFFADYRPSFRKEAADDGW
                     KRCLEWMKANGVG"
     gene            13423..14466
                     /locus_tag="PK264_08800"
     CDS             13423..14466
                     /locus_tag="PK264_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76025.1"
                     /translation="MHRRAFALLAAGSVMALGVTLTLAPSATAQSPDEMAAAAKKEGK
                     LTLYHNIRPAGIEVILQKFREKYPGIQTEQIRLGSGPLNERFRTEYDAGRNIADVLIT
                     FPDKPFMDKAPGWFAKWQPAEHKANFAPETSIGDYLFVLQQVRESIVWNKNLVKDADA
                     PKEWADLLDPKWKSKVGLNTPWRSISVQQMFAYWQSIGIKDAAERMKANEVRFFEGSS
                     GIIQAIIRGDIHVAHMTDIPLNAMLADGAPIGFTYPKSGTTLSNAVAGVAAKAPHPNA
                     ARLFLNWLMSAEGQALLVAEGGLSVTRKGAPALSHLPATAALPKTVDGMSVLTPDVQK
                     PLIEHWRKVFGVK"
     gene            14508..>15729
                     /locus_tag="PK264_08805"
     CDS             14508..>15729
                     /locus_tag="PK264_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD76026.1"
                     /translation="MSGGTAMTALYRTVYALVALIVVIPIAALIYGSFRTDAPLAPGG
                     AWTLRNWVGLFSEGVLSTIATTVWIGIATAIFSVVFGTAIALVVHRTDFKYPNTITAL
                     IGLAFYFPSFILAMAWIIIGSPGGVINYVWGTLLGLPGRMDIYTTWGIVLVMVLHQVP
                     FVYLTMRGPITAMDGIYEEAARTAGASPRQVLARITLPLLGYSIASSAILTFITSIEQ
                     FAIPTLIGSPGRIHVLATQLYILCRFPPTDYGLAAAIGLALSAITGLAILAQRWLVGS
                     TRLATVGGKAGRLAPIRLGRWKWAAYLLTFGFAFLALVLPAIILVYTSLVRFFVANPF
                     QAAYTGRAYTFIWQSAATLASFNNTLIASGTAAALGSALGFLVSYFTLRQRPRGSRAL
                     DAIASLPFGVPGIVI"
     assembly_gap    15730..15756
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15757..16168
                     /locus_tag="PK264_08810"
     CDS             <15757..16168
                     /locus_tag="PK264_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD76027.1"
                     /translation="YGTLFILIVAYITRYLPYATETIGGQMVQIDKSLEEAAWVSGAT
                     RIEALAKVILPIALPSVQGAFFLLFMAFFREISSAVLLFSASTMVISISIYAFFEQAN
                     WPLASALSVIATLIIFLVMTLILLLSPHVRRRWT"
     gene            16188..17240
                     /locus_tag="PK264_08815"
     CDS             16188..17240
                     /locus_tag="PK264_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76028.1"
                     /translation="MVRDLVKIYGTNRVVDRVSFTVGKGEFVGLLGPSGCGKTTTLRA
                     IAGLEEADGGTISIDGASVSDKAVGLAVPPHVRDVGMVFQSYAVWPHMTVAENVAFPL
                     KVKRQPTKVITDRVAWALGIVGLTELRDRQPSQLSGGQQQRVALARAIVGRPKALLFD
                     EPLSNLDAKLRDQTRAEIRRLQKELGVAAVYVTHDQAEALSMADRVIVMSGGRIMQEG
                     PPKELYRNPANRFVADFVGTASFLDVTVVPGRGYALKGGQVLAFDTDGSPNGPVQLML
                     RPEQIALWPPGHPTPAASEAIAGKVREAHFLGAFTEYVVDVAGGTVKVHAMEDVAPGA
                     ETLLVFARAAGRIIPV"
BASE COUNT         2830 a         5751 c         5671 g         2915 t
ORIGIN      
        1 tctccaccga ggtcttggga tcgagcgagg aggtcggctc gtccgcgagc agcagctcag
       61 gcttgccaat gactgctcgc gcgatggccg cgcgctgctt ctctccgcct gagagcacgg
      121 atgggtaggc atgcatgcga tcgccgaggc cgacccactg cagaaggtcg gtcacgtcct
      181 ccttgtagtc ggcgatgcgc tttccgacca ccatgagggg cagggcgaca ttctcccagg
      241 tcgtcatgtg gtcgagcagg cgaaagtcct ggaagacgat gccgatacgg cggcggagct
      301 gggcacgtcg cgccggcgtc acggctgaca cttcctgccc gaacaactcg acgcgaccgg
      361 aggtcggctg ctgcgacatg aagaggaggc ggaggagcga cgtcttgccg gctcccgagg
      421 cgccggtcag gaagtggaac gagcccggct gcagatggaa ggtgacgcca cgcaggatct
      481 cgatcccgcg cccgtacttc aacccgatgc cgttgagctt gatcacgatg gatgtgcctg
      541 tgccgccggc cggggccaga aaccctcagc cgtgcccctt atgaccaatc agccttccgc
      601 gatcaatcgg acttgagtgt ggccgacgga gcgaacagcg catccgcgcg ctcgcgcgga
      661 gtctgctcgg gcccttgcgg ggtgatggcc gctcgtgcca tcgctaaccg gcgtaacctc
      721 gagctggtgg tgatccagcc tggcaaaggc agattggaca tgacggcggc agacacctct
      781 ggcgacatcg tcacggcagc gctcctcgtc atcggcgacg agatcctgtc cgggcgtacc
      841 aaagacaaga acatcggcta catcgccgac cactgcacgc agatcggcat ccagctgcgc
      901 gaggtgaggg tcgtcgccga cgacgagggc gagatcgtcg cggcgatcaa tgcgctgcgc
      961 gcgaagttca cctacctctt cacgaccggc ggcatcggcc cgacgcatga cgatatcacc
     1021 gcggattgcg tcgccaaggc gttcggcgtg aaaatcgatc acgaccagcg cgcgctcgac
     1081 ctcctggtcc cctatttcaa gaagcgcggc gtcgaggtga cgccggcccg gctcaggatg
     1141 gcgcgcatcc cggacggcgg cgagctcgtc cacaactcga tctcggtggc gccgggcttc
     1201 atgatcggca acgtcatcgt catggccggc atcccgagcg tgatgcaggt gatgctcgac
     1261 gatgccacca agcgcatcag aacagggcga aagatgctgt ccgcctcgat ggacctcaat
     1321 cggcccgaag gcgagatcgc cgagctcttc gaggcgcacc agaagctcta tccggacgtg
     1381 ccgatgggca gctacccctt cttccgtgac aacaagccgg ggacgcagct cgtgctccgc
     1441 tcgctcgacg ccacccgcct cgccgaggcg gaggcggggc tccgcgagaa gctcgcggcc
     1501 aaggggtgga tttgacggtc aagcagggcc atgcggcaaa cgatctcgac catcgtcttc
     1561 gatgtcggcg agacgctgat cgacgagacg tcgatgtggg cggggtgggc gcgctatttt
     1621 ggcgtcacgc tcgaggcgtt tcttgccgag cttgatctct cgatcgcgga ggggcggcat
     1681 catcacacgg tgtatcgccg cttcaggccg gacttcgatc tgaaggaagc gcgcagggag
     1741 cggacagcgc gcggcgagcg cgacatcttt tcggccgccg atctctaccc ggatgcgctg
     1801 ccggcgctcg ccggtctgcg caaaggaggc tttcgcatcg gtatcgcggg aaaccagccg
     1861 cgtgcgtcgg agtgggcgct gcgtgaagcc gggttcgagg cggatttcgt cgcctcctca
     1921 gaggcctggg gcgtagagaa gccgtcgccg gcgttcttcg ccaaggtcgc ggaggctgcg
     1981 ggggagccgc cggggcacat cgcctacgtc ggcgatcggc tcgacaatga tgtgctgcct
     2041 gcgcgagcgg ccggcatgac ggcgatcttc ctggtgcggg gtccgtgggg acgggcgcac
     2101 gcgcgccgcg cggacgcggc gctcgcgcat tgcacgattc atggtctggc cgagatcgct
     2161 gcgctcgtca gatgaggatg tcgtgggtgc ggcgccggaa ttttttccag tacgagtgaa
     2221 ccacggcgac ggatcgtgag gcctcgcacg ttgacgtcgt cgggttggaa ctcgccggca
     2281 ggcgatgcca gcctattcga ccggttggca attttctcgc caggccgcac ttgattttgg
     2341 cctgcgggca gttgccgacg attgcttatc cgggatgttt tgccatgaca catgtttgcg
     2401 ccgatgcctc atgccgccac gacctcgact tccatgcgag cgacgaccgc ccctcgcgtc
     2461 gccgtgcgct gatcctcggc ggcagcgccg cggcgatcgg cggcgggctt gtcacggcca
     2521 ccacgggcgc ctccgcccag gtcggggacc agtcgtcttg gcggttctgc aacaagtgct
     2581 tcacgctgtt cttcgacggc tatccgacca agggaagctg cccggccggc ggagggcacg
     2641 tcgcccaagg ctacatcttc aatctccatc acgacgtgag gaagcagccg ccgcgtgtca
     2701 cgggccgttt cgcctggcag tacgattggc gcttctgcaa caagtgcttc gccatgttct
     2761 tcgacggata tccagcgaag gggcgctgtc cggccggagg cggccacgtc gcgcaaggct
     2821 tcaatttcgg gctcgacttc accaatgaaa gccagcgccg tccacccgtc ggcgtgtttc
     2881 agaccacgtg gcgcttctgc ggcaagtgca acggcatgtt tttcgacggc tacccgacca
     2941 agggccgctg tcagggcggc ggcgcgcatg ccgcggcagg gtggatgttc cacttggcct
     3001 tcacggccgg accggcgaca ccgcctccgc caccgcctgg gacgggtgca ggcctcggca
     3061 atgcgatcgt cgctttcgcg cgggcgcgcc tgaaccagtg cgtcgacaca cgcttgcaga
     3121 cgcggccgca ggcctgtccg gcattggcac ccggagcggt cggcgatggc gagtgcacgc
     3181 atctcgtgca ggcggcgctt gcctcggtcg gtgcgcgcgc gccgaccttc cagcccggca
     3241 actacgtctg gggtaacgtg atcaccggag gctatcagcc gggcgatatc atccagttct
     3301 acaatgcccg cttcaacggg ccgaacagct ggtggcagac ggcgacgcag cacacggcga
     3361 tcatcgaggt gatgcgcagt gcgactgccg tcacactctt gcatcagaat tcgccgatcc
     3421 gcgccgtgac gcgccaggat ctcgacctcg cctggccgca taccggtcgg ttcgtcgtgt
     3481 tccgggcggt cagggcgtga tggcgtaagc gcgatcagcg accagcctcc agcacgatct
     3541 tgcactcggt tggaagctgg tcgagcgtca gcggtggcgg cggtggcggc ggcggcttcg
     3601 ccggcgttcc gggaggaagc gggggtggcg gcgggcgcga gaggagcttc atccagtcgg
     3661 cgagttcctt gccgcagcca tcagcgtcgg ttggaggcgg cgccaccgcg aactgcgccc
     3721 ggcaggtcgg gttttcggcc gggcagccca tccggatgtg gaagtgatag tgatggcccc
     3781 agatcgggcg cacctttttc aaccaggccg cccgtgcggg gtcggcaggc acaccgtcgc
     3841 acatcgcttt cttgattgcg ggatggacaa ggacgcgctc gacttctggg taactcgcgg
     3901 cgcgcctgat gagcttcaca tgggcctcgg tccacttcgt ggggtcgacg gccaggttgt
     3961 ccgcgcggag caccgagatc gcgctcatct cctcgcgctc ctttttttcg aggcggcggg
     4021 gcggcatcgg catcagccag atatcggcat cgaggccgat ctgatggctc gcatgcccgg
     4081 tcagcatcgg accgccgcgc ggctgcgaga tgtcgccgac taggaggccc ggccagccgt
     4141 cgtgggcctg cgcctccttg gcgagcttct cgacgagcga gaccagcagg ggatggcccc
     4201 agaagcggtt ccgcgagagg cgcatggcct gccatgccgg cccgtcgatg gcgagcggca
     4261 cggcaccagc gaggcagcct ttggcatagg agccgatggc gcgggcggcg agattggcgg
     4321 gcgccgtctg atggccgaac agctccttgg cgggcaccgg tttcggcaga ggtgtggcgg
     4381 ccttggcagg cgcggccgct gcggctgcgg gctttgccgg cgcgaccggc ttcggtgagg
     4441 cggcggcctt gggttgcggt gacgccggag ccgccttggg tgctgccgcc ttgggtgccg
     4501 ctttcgcaac gggcggcacg accacgggag ctttgggtgc ggttgtcgcg gctgccggat
     4561 ctgccacggg tggtggcggg atatcgggtg tcgcaaccga ctgagccctg acgtacaggc
     4621 tgatgccgcc gggaagcagc acgatcgcga ccatggtcag tcccgcgagc agccaacgcc
     4681 gcattgcagc gctcctcgtt gccgaacagc gcccgactat aggcacactc gggcaggaat
     4741 tggacgctct ctctccgcgc gcgtgggctc gtggtcgcaa gtacgcgcga cagttttgcg
     4801 gttcggtcag gcgcgggcga cgcgcccctg cacctcgccg aagccgtcga ttacgccgcg
     4861 gatgtcgtgg ctgccgtgca accagttcat gccgatgagg cttcccgtga tgacgatctg
     4921 gcccgcctta agctgcttgc cgtgcgcggc cagcgcgggc gccagcacct cgagggcgcg
     4981 gaagggatcg acgctcttcc cctcgatgtg caccttgccg tcgacaaaca ggcggccggg
     5041 ccgcgacttg tgctcctgtt tgcgccagtt tgcgatctcg gggccaacca tgatgcccga
     5101 gttcaggatg caatcggcga ctgccacatg gcgcggtacg ctgtcgggat tggcgttgcg
     5161 gctctggcag agctcgaaga ccacatggcc cgcaccaacg cagtcgagca cggaggaacc
     5221 tgtcgcccct ttcggcacgt cgcgagccat tctgaaggcg atctcgagct cgatgccggc
     5281 gggctccgag cccttcacgc tccaggtgcc gccggccgga acgcggcccg actcgagtag
     5341 cggcgcgtaa atgacggggc ccccgttcgg catctcaatc accttccagc ccatcaccgg
     5401 cgagccgagt gccgccacca ccttgtcctg gaccgcaaag ccctcctcga acgtcttcgg
     5461 cgcatccacg agcgtgatgc gtgcgccggc cgtgcgggcg gcgacgagct ttgcggcgat
     5521 ggcggacagg cgatcggtag acatgaggca gctcctcgcg accttgctca tggctaccta
     5581 acgcccgaag cagcatgggg agaatcgctt ttttgtcgag gcagggtcgc gaatcgttga
     5641 ccggcgccac aatccacgct ttgatcgctc atgcacaatt tgcatagcag catcggcagg
     5701 aacggtctga ggaaagcgac atgggcggcg ccaggcatca tcggcttgtc atccttggct
     5761 ccggccctgc cggatacacg gcggcgatct atgcggcccg cgccatgctg aagccgctcg
     5821 tcatccaggg catccaaccg ggcggccagc tcaccatcac gaccgacgtc gagaactatc
     5881 cgggcttcgc caaggtgatc cagggcccct ggctcatgga ggagatgaag gcacaggccg
     5941 agcatgtcgg caccgagatc gtcatggatc aggtggtgaa ggtcgagctc gcgagccgcc
     6001 ccttccgcct cacaggcgac agcggcgacg tgtggacgtg cgatgccctc atcatctgca
     6061 cgggcgcgca ggcgcgctgg atgggaacgc catcggagga ggcctacaag ggcttcggcg
     6121 tctcggcctg cgccacctgc gacggtttct tctacaaggg ccgggaagtg atcgtcgtcg
     6181 gcggcggaaa ctcggccgtc gaggaggcgc tgttcctcac gaagttcgcg agcaaggtga
     6241 ccgtcgtgca tcggcgcgac agcttccgcg ccgaaaaaat cctgcaggag cggctcttca
     6301 agaacccgaa ggtcgaggtg gtctggaaca gcgtggtcga ggagatcaag ggcccgaccg
     6361 atccgcgcac ggtctcgggc gtacggctca aggacgtcaa gacgggcaag gtcaccgacc
     6421 gcaaggtcga cggcgtgttc atcgccatcg ggcatgctcc agcgaccgag ctcttcaagg
     6481 gccagctcga gatgaagcca tccggttacc tcattacacg gccggattcg acggcaacct
     6541 cgatccctgg cgtgtttgcg gccggtgacg tgaaggacga cgtcttccgc caggccgtca
     6601 cggcggccgg catgggctgc atggcggcgc tggaagccga aaagttcctc gccggtcacg
     6661 gcgcggccgc acacgcggcc gagtgagcag tgcactggga ttacggcccc tcgaacggcg
     6721 atcgatgacg atggattggg acaagctgcg catcttccac gctgctgccg aggccggcag
     6781 cttcacgcat gcgggcgaca gcttgcgcat gagccagtcg gcggtgagcc ggcaggtctc
     6841 cgcgctcgag aaggacttgg gcctcgccct cttccatcgc catgcgcgcg gcctggtgct
     6901 gaccgagcag ggtgaggtgc tgttccgcac cgcgtccgag gtgatgacca agctgcaaac
     6961 cgccgagacg ctgctttccg acgccaccac gaagcccgcg ggtgatctcc gcatcacggc
     7021 gaccgtgagc ctcggctcgg tctgggtgac gcagcgcatc cgcgagtttc tcgaccttta
     7081 tcccgaaatc cgcatcgaac tcgtgttgaa cgacgagccg gtcgatctct cgatgcgcgt
     7141 tgccgacgtg gcgatctggg cgcgcgagcc ctcgcagagc gatctcatcc gtcggccgct
     7201 cttccatatg cagatccatg cctacgcgtc gggcacctac atccgcaagt tcggcatgcc
     7261 gaagacggtc tccgacctcg accagcatcg cctcgtctcc tacattggca acccgccgca
     7321 gcacctcgcc gcgatcaata cgctcgagag catcggccgc gagagccagt cgccgcgcgt
     7381 gccggcgttc cgtgcgagca cggtgctcgc gctcaagtat gcgatccggg ccggcctcgg
     7441 catcggcctc atcccggact acctgaccga ggaggagggc gacctcgtgc cggtgctgca
     7501 ggagctcgcg gcgccgaagc tgcccgtcta tttcgtctac ccggaagagc tgaagaccgc
     7561 caagaaggtg caggtcttcc gcgatttcct ggtgtccaag gggcggcagt ggaagttttg
     7621 atcgtcacat aatgcttcgc cccgccgcct cgagcgtgtt gcgcagcagg caggcgattg
     7681 tcattggacc gacgccgccg gggacaggcg tgatcgcgcc ggcgacgcgg cgggcctcct
     7741 cgaaggcgac gtcgccgacg agacggtccc ggccatcggc gcccgcaatg cgattgatgc
     7801 cgacgtcgat cacgatcgcg ccgggcttga gccaatcgcc gcgcaccatc tcgggtttgc
     7861 cgacggccgc gatcacgaga tcggccctgc gcacgacgga cgacagatcg cgggtgcgcg
     7921 aatgggcgat cgtcaccgtt gcattctcgt tgagtagaag ctgcgccacc ggcttgccga
     7981 caatcgtcga gcggccgatc accacggcct ccatgccggc gaggctcggc gccacggtct
     8041 tcgcgaggat caccgaaccg agcggcgtgc acgggacgag cgccggctcg ccgattgcga
     8101 gccggccgac gttcaccgga tgaaagccgt ccacgtcctt tccgggagaa atcgccgcga
     8161 tcaccttacg cgcatcgatg tgcgcgggga gtggaagctg cacgagaatg ccgtcgacgg
     8221 cgggctcggc attgagcctc gcgaccagcg ccagcaggtc cgcttccgag gtggtcgcag
     8281 ccagcttgtg ctcgaaggac agcattcccg cctcgagcgt ctggcggccc ttgttgcgga
     8341 catacacctc gctcgccgga tcggagccga cgagcacgac ggcgagcccc ggcgcgcggc
     8401 cgccgcgggc gacgatgtcg ccgacaaccg tcttcacctc ggcgcggatg cgggccgcct
     8461 cgaccttgcc atcgatgatc cgggcttctg ccatggcacg cctcgcttca ctcaagactg
     8521 ggatttcaag cgctctccga cgcgcggcgc aagtgctgcg gcatcgccgg tgacagcgac
     8581 ggtcttcacg cgcgatttgc cgccgcgctc gacctcgacc gtgcgccgcg gcacatcgag
     8641 ccagtcggcg atcagacgct cgagcgcggc attggcctcg ccgtcctgcg gggcggcgcg
     8701 cacgcgagcc ttcagcgccg gcccttcggc cgtcatctcg acgccttcga tccggtcggc
     8761 ggacgatttc ggcgtcagac ggacgcgaat gatgaggcga cccgtggccg cgcgatacag
     8821 ctcgccgccg gccgccatgc tcagagcatt ccggcgatag tcggcagcag ccagctctgg
     8881 atcaccatca ggatgacgat caggatgaca ggcgagatgt cgatgccgcc gagatcggga
     8941 agcaggcggc ggatcggacc gagcagcggc tcggtcacag cgaccagcat ctgccagaac
     9001 gagcgcacca tctggttgtg cgggttgacg accttgaaca ccacgagcca gctgaagacg
     9061 gcactggcga tgatcacata gatatagagc tggatgatgt agtggacgaa gggcaggact
     9121 tcgatcatcg atcgctttcc gtggttggcc tccggaatcc gcggacgtgt gccagcggcg
     9181 gacgggcagt gttcgacgca cctgattagc taacggcggc gacaggggca agaaacggct
     9241 tggcgggcgg gtgacatcct tgtgaacagg cggcaatgtc cccagatctg cagacatgaa
     9301 agacctcagt tgtcaggagc aaccgtgatc tggcgatggc tctcgcgggc ggcgctgatc
     9361 ggcgcaatcg cagcgcatcc gcgcctcagg cagaccgtgc tgctcgccgg cggagtggtt
     9421 gcgttcggtc ttgcctactg gttcctctcg agcttcttcg cgacgctcga tcagatcgcc
     9481 aacccctggc tgcagctcgc cttcacaatc gcgaccgcgc tgctggtcgc tctcgcctcg
     9541 gtcgcggccc tcgttatcct gggtgcgtgg tcccgtgtga aggtgcaccc ggcggcgcgg
     9601 tcgccatcgc gagagcggct cgatgagctt tttgccaagc acggaatcga ggcaccttcg
     9661 atcaccgtca tgccggcggg gcgtgccgcg gacggtcccg ccgtcgcggt catcgggtcg
     9721 ccgggcgtcg gtcgcacgac actgatccga gcgctgtcgg aggaactggc gtcgcgggag
     9781 cgaggcgcaa acccgcgctt cgtcgagctg ccaccgctcg aggccgatcc gatccgtcaa
     9841 ggcgatgccg tcgtcatgag cgccgacttc gatcaggtcg tgctgatcgt cgcagatgat
     9901 ctgaaggccc acgagatggc agcgctcgag acgatcgcga aagccggcaa gcgggcgatc
     9961 gtcgtgctcg cgaaggccga tgccctgcgg ccgggcgagc gggcggagct ggtcgcggcg
    10021 ctcagcgagc gggttgcggc tcttcccggg ccgcccgtcg tcgtggcggc aagtgccgat
    10081 ccggcaccag tcgtcgcgga gctggtaacg gccgagggga cagtcaagac gaccgagcgc
    10141 cggccaccgc gatcgatggc gccgctggcc gacgccatcg aggcggcctt gacaaggcga
    10201 ggccaagcga ggctcccatg acccgagaca gatcatttac tgatgccctc gacgctctgc
    10261 gtcgcatcgc ggacagcccc gagctcaggc ccggcgagcg caaggcggtc gcgcaggagc
    10321 tgcgcgatgt cgaagccatg cgcgacaagc tggccgaggg ccgtgtcgag atcaccgcgt
    10381 tcggcgagat caacgcaggg aagagcgcgc tcctcaatgc gctgctcggc cgcgaggtgt
    10441 tcaaggtggc tgcccgcggc ggcgagacca aggtgcgctc ggcggaggaa tggcgaccgg
    10501 acgtgcgcga gctttcgggc ctcggcacaa agctcgtggt ggtcgacacg ccgggtctca
    10561 acgaggtcga cggcgcccat cgcgcccgta tcgccgagcg cacggtgcgc cagtcggaca
    10621 tcgtgctgtt cgtcgtgcgc ggcgatctca acgacgtgga gatctcggcg ctgcgcgaac
    10681 tctccgccct ccacaagccg ctgatcctgg tactcaacca gatcgaccgc ttccgtaagt
    10741 ccgagctcgt cgaggcgctg gccgcgatca agtcgcgggt cgccggcctc gttgccgatc
    10801 gcgatatcgt cttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnncc tcgcccgcga aggcaaggcc gtcgccgctt tgaacgcgag tctctttgcc
    10981 gccgacgtct cgcagcggat cgcccgcctc aaggcggatg cacggcaggc cgaggccgag
    11041 gcgatcatcg agcgctacat gctggtgaag gccagcgcgg tcgctctcaa tcccgtgccc
    11101 gtgctcgaca tcatcggctc gatgggctcg gacgcggtga tgctgaagca cctggcaagc
    11161 gtctacggac aggagatcac gacggcgaac gcggggaagc tcgcgcagga catcatgggc
    11221 gcttggggca tcgctatcgc ggtgcagtgg ggcacgcaga cgctgctctc gctcatgaag
    11281 ctcaagacct tcggcctcag cacgatcatc acggccgttc cgcaaggcct cgcggcagcc
    11341 tggacgacgc acgtcgtcgg caagtcggcg cacgtctatt tccgagaggg aggctggggt
    11401 gcgggcggcg cgcgccaggt gatcaaggac gtactcgcga gctcgtcgcc cggcagcatc
    11461 ctcgagccgc tgaaagcgcg cctcgaagca cggctcagag gccgatcggc atgacgggca
    11521 atgctcaatg tgcaaggagg tcgatctcgg cgaagcctgc gagatcgagc cgcgcgcgca
    11581 cggggagata gcggaacgtc gcttccgcga gcgcggtccg cccctcgcgc gccagcatgt
    11641 cgtcgaggcg ggatttcagg gcgtgcaggt ggagcacgtc cgaggccgca taggcgagct
    11701 gctgctggct gaggctcgcc gcaccccagt ccgagctctg ctgctgcttg gagagatcga
    11761 tgcccagaac ctcgccgacg aggtccttga ggccgtgccg gtccgtgtac gtgcgcgcga
    11821 gcttggaggc caccttcgtg cagtagatcg gcgacatgga gaggccgagg tagcgctcga
    11881 tgacgccgag gtcgaagcgg gcgaagtgca ggatcttcag gacggccgga tcgccgagca
    11941 gcgccttcag ccgcggcgcc gaccagtcgc ggccgtcgaa ctgcacgagg tgcgccgtgc
    12001 cgtcgcccgc ggaaagctgc acgaggcaaa gccggtcgcg gtgcgggagg agccccatcg
    12061 tctcggtgtc gatggcggcg cttcccccga aggagagccc ggcggggagg tcgttcttgt
    12121 gcagaaaaat gcgcgggctc gacatcggtg tctcctgagg ctgccgcgct tgtcccgcac
    12181 ggcccgacga gcgtcaagcc gtggctgacc agccggcatc cggctcactc cgtcgtcacg
    12241 aggagatcgc ttacgggtag cacggcgacg ccgagcgcga atagccgcgc tcgtcggagc
    12301 ctaccctcaa ttcaccaagt ccattgctcc acagtctctc atccgacccc gttcgctttc
    12361 atccattcga ggcaccgctt ccaaccgtcg tccgcggcct ccttgcggaa gctcgggcgg
    12421 tagtcggcaa agaaggcatg cggcatgccc tcgtagacgt ggatctgcga cttcgcgttg
    12481 ctgccgaacg ccttgcgcat ctcctcgacg gtcgtgactg gaatgccggc atcggcgccg
    12541 ccgtagagtc caagcaccgg tccgttgagc tttgccgcaa cgtcgaccgg atacgtcggg
    12601 ttgttggcat tgcggtcgcc gacgaggcgg ccgtaccagg caacgccggc cttcaccttc
    12661 ggattgtgtg cggagtaaag ccaggtgatg cgccctcccc agcagaagcc ggtgacgccg
    12721 agcttgctgt cgtcgccgac gccagctgct ttcgcccagg cgaccgccgc gtcgagatcc
    12781 gccatcacct gcgcgtccgg caccttggcg acgatcgggc ggatttcgtc gatcgacttg
    12841 agcttcgcga cgtcgccctg gcgctggtag agttcgggcg cgatcgcgca atagccgagc
    12901 ttggcgagcc gccggcacac gtccttgata tgctcgtgga cgccgaagat ctcctgcacg
    12961 acgaggatga ccggccggcc cttggcgcct gcaggagccg cgcggtaggc ggcaattgca
    13021 tccgtcccgg atttcacctc gatcatgccg gcatcgaggc cggtcgcgtc cgtgacgatc
    13081 atggtctgcg cgacgaccgg ctgcacggcc acggcaaatc ccgtgacgat gccggttgcc
    13141 gcgcccgtcg ccagcacctg ccggcgtgag aggtcggcaa tcttccagcc cggatcatga
    13201 aacgacatgg gtgactcctc acggttgtgg ctcgtgcgca aatgtcgctg aaggttggcc
    13261 gtcgttcaag tcgcgacagt tgcaccggac attgacattc gcgctgccgc tgtccgaagg
    13321 ttgatcgcaa gaggcaactg agaccttggg gcgctcgtgc ggcgcagctg cgaaacgact
    13381 ggaggaaaca atcggatggc ttccactcta ttggggcgtc gcttgcatcg ccgcgccttc
    13441 gccttgctcg cggccggctc tgtcatggcc ctcggtgtga cgctcacact tgcgccgtca
    13501 gccaccgcac agagccccga cgagatggcc gccgcagcca agaaggaggg caagctcacc
    13561 ctctatcaca acatccgtcc cgccggcatc gaggtgatcc tgcagaagtt ccgcgagaag
    13621 tatcccggca tccagaccga gcagatccga ctcggctcag gaccgctcaa cgagcgcttc
    13681 cgcaccgagt acgacgccgg ccgcaacatc gcagatgtgc tgatcacctt ccccgacaag
    13741 cccttcatgg acaaggcgcc ggggtggttc gccaaatggc agcccgccga gcacaaggcc
    13801 aattttgctc ccgagacgag catcggggac tatctgttcg tgctccagca ggtgcgcgag
    13861 tcgatcgtct ggaacaagaa cctcgtcaag gacgccgatg cgccgaagga gtgggcagac
    13921 ctgctcgacc ccaaatggaa gagcaaggtc ggcctgaaca cgccctggcg ctcgatctcg
    13981 gtgcagcaga tgttcgccta ctggcaatcc atcggcatca aggatgcggc cgagcgcatg
    14041 aaggcgaatg aggtacgctt cttcgaaggc tcgagcggca tcatccaggc gatcatccgc
    14101 ggcgacattc acgtcgccca catgaccgac atcccgctga acgcgatgct cgccgacggc
    14161 gcgccgatcg gcttcacgta tccgaaatcg ggaacgacgc tctccaatgc ggttgccggc
    14221 gtcgcggcca aagctcccca tccaaacgca gcgcgcctgt ttctcaactg gctcatgagt
    14281 gctgaaggcc aggcgctcct cgtcgccgaa ggcgggctct ccgtgacacg caagggcgcc
    14341 ccggccctgt cgcacctgcc ggcgaccgcg gcgctgccca agacggtcga cggcatgagc
    14401 gtcctcacac ccgacgtgca gaagccgctg atcgagcact ggcgcaaggt tttcggcgtc
    14461 aaatgacgcc acccgccctc gaggctccga cgccggcccg atcgccggtg agcggcggca
    14521 ctgccatgac ggcgctctat cgcaccgtct atgccctcgt cgcgctgatc gtcgtcatcc
    14581 cgattgccgc gctcatctat gggagcttcc gcaccgacgc gccgctcgcg cccggcggcg
    14641 cgtggacgct ccggaactgg gttggtctct tctccgaagg cgtcctctcg accatcgcca
    14701 ccacggtgtg gatcggcatt gcgacggcaa tcttcagcgt ggtcttcggc acggcgatcg
    14761 ccctcgtcgt gcaccgcacc gacttcaagt acccgaacac gatcaccgcg ctcataggcc
    14821 tcgcgttcta tttcccctcc ttcatcctgg cgatggcctg gatcatcatc ggctcgcccg
    14881 gcggcgtgat caactacgtc tggggcacac tgctcggcct ccccggccgc atggacatct
    14941 acacgacctg gggcatcgtg ctcgtcatgg tcctccacca ggtgccgttc gtctacctca
    15001 ccatgcgcgg gccgatcacc gccatggacg gtatctacga ggaggcggcg cgcacagccg
    15061 gcgcgagccc gcggcaagtt ctcgcccgca taacgctgcc gctgctcggc tactcgatcg
    15121 cctcgagcgc gatcctcacc ttcatcacct cgatcgagca atttgcgatc ccgacgctga
    15181 tcggctcgcc cggccgcatc catgtgctcg cgacgcagct ctacatcctc tgccgcttcc
    15241 cgccgaccga ctatggcctc gccgccgcca tcggcctcgc gctctccgcc atcaccgggc
    15301 tcgccatcct cgcgcagcgc tggctcgtcg gcagcacacg cctcgcgacc gtcggcggca
    15361 aggcggggcg cctcgcgccg atccggctcg gccgctggaa atgggccgcc taccttctca
    15421 ccttcggctt cgccttcctc gcgctcgtcc tgccggcgat catcctcgtc tacacctcgc
    15481 tcgtccgctt cttcgtcgca aacccctttc aggccgccta cacgggccgt gcctacacct
    15541 tcatctggca gagtgccgca acgctcgcga gcttcaacaa cacgctgatt gcctccggaa
    15601 cagcagcagc gctcggatcg gccctggggt tcctcgtttc ctattttacg ctgcgccagc
    15661 ggccgcgagg atcgcgcgca ctcgatgcga tcgcgagcct gcccttcggc gtgccgggca
    15721 tcgtcatcgn nnnnnnnnnn nnnnnnnnnn nnnnnnatac ggcacgctct tcatcctgat
    15781 cgtcgcctac atcacacgct atctacccta tgcgaccgag accatcggtg ggcagatggt
    15841 gcagatcgac aagagcctcg aggaggcggc ctgggtctcc ggagcgacgc ggatcgaagc
    15901 actcgccaag gtcatcctgc cgatcgcgct cccgtccgtg caaggcgcat tcttcctgct
    15961 cttcatggca ttcttccgcg agatctcgtc ggccgtcctc cttttctcgg catccacgat
    16021 ggtgatctcc atctcgatat acgccttctt cgagcaggcg aactggccgc tcgccagcgc
    16081 cctctccgtg atcgcgacgt tgatcatctt cctcgtcatg acactcatcc tgctcttgtc
    16141 gccgcacgtg cggcggcgct ggacgtgagg agttcctctt gagcatcgtg gttcgcgacc
    16201 tcgtgaagat ctacggcacg aaccgcgtgg tcgaccgcgt ctccttcacg gtcggcaagg
    16261 gcgagttcgt cggcctgctc ggtccatcgg gctgcggcaa gacgaccaca ctgcgcgcca
    16321 tcgcaggcct cgaggaggca gacggcggca cgatctccat cgacggcgcc agcgtctccg
    16381 acaaggccgt cggcctcgcc gtgccgccgc acgtgcgcga tgtcggcatg gtgttccagt
    16441 cctatgcggt ctggccgcac atgacggtcg ccgagaacgt cgccttcccg ctgaaggtga
    16501 agcggcagcc gacgaaagtg atcacagatc gtgtcgcctg ggcgctcggc atcgtcggcc
    16561 tcacggagct cagagaccgc cagccttcgc agctttcggg cggccagcag cagcgcgtgg
    16621 cgctcgcgcg agcaatcgtc ggcaggccga aggccctcct cttcgacgag ccgctctcga
    16681 acctcgatgc gaagctccgc gatcagaccc gcgccgagat acgccgtctg cagaaagagc
    16741 tcggcgtcgc cgccgtctac gtcacgcacg atcaggccga ggcgctctcc atggccgacc
    16801 gcgtcattgt catgagtggc ggacgcatca tgcaggaagg cccacccaag gaactctacc
    16861 gcaatccggc gaaccgcttt gtcgccgact tcgtcggcac ggcgagcttc ctcgacgtga
    16921 cggtcgtgcc cggccgcggt tatgccctga agggcggcca agtcctcgcc ttcgacaccg
    16981 acggctcgcc gaacggcccg gtgcagctga tgctgcgtcc cgagcagatc gctctctggc
    17041 cgcccggcca tccgacacct gccgcctccg aggcgatcgc cggcaaggtg cgtgaagccc
    17101 acttcctcgg tgccttcacc gagtacgtgg tcgacgtcgc gggcggcacc gtgaaagtgc
    17161 acgccatgga ggacgtcgcg ccaggcgccg agacgctcct tgtgttcgcg cgcgccgccg
    17221 ggaggatcat accggtctga tgttcggttt tgcgccctcc accccgtcat gactgggaag
    17281 gaggtcccat acggctcctc gcccaaat
//
LOCUS       DAORNH010000065        17003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2553_length_17003_cov_2.374026, whole genome shotgun sequence.
ACCESSION   DAORNH010000065 DAORNH010000000
VERSION     DAORNH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17003
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_17003_cov_2.374026"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..394)
                     /locus_tag="PK264_08820"
     CDS             complement(116..394)
                     /locus_tag="PK264_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76029.1"
                     /translation="MTEPEQPPTPLPPDRDHWLVRPSTIRLLWISGSLLLAALVALDL
                     VVEHKPHFGIDGTFGFGAWFGFAACVAMVLFAKLLGVLLKRSDSYYDH"
     gene            complement(391..1896)
                     /locus_tag="PK264_08825"
     CDS             complement(391..1896)
                     /locus_tag="PK264_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HRD76030.1"
                     /translation="MRLPWSPDTLLALALIIPFIGALLIPVFHRWPNLREAVTLATAA
                     KLAVVVWSLLPHVLAGQQPERTLLDIVPGLTIAFNIEPLGMLFALVASSLWIVNSIYS
                     IGYMRANDEPRQTSFYVCFAVAIGSTIGLAFAKNLSTLFLFYELLTLSTYPLVTHKAS
                     DEAVRGGRIYLMLLIGTSMLLLLPAIVGTGFLAGTMDFKAGGILPASLGAGAIGALLA
                     LYVFGIGKAALMPLHFWLPAAMVAPTPVSALLHAVAVVKAGVFTVLKVIVYVFGVERL
                     AADGAAWWLVWAAGGTVVLASMVALRQDNLKKRLAYSTVSQLSYVVLAVAILTPISAI
                     GAAMHIAAHAVAKITLFFAAGAIYTAAHKTEVSELDGIGWQMPWTMTAFAIGALGMIG
                     LPPTAGFLGKWFMLSGAFATQQWLPIGVIVLSTVLNASYFLPILFRAFFVEPARGHAV
                     GAAATHHDDHGEAPLPMVIALTVTAGATIVLFLAPDIPLALAKLMLTGVAR"
     assembly_gap    2052..2081
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2082..3382)
                     /locus_tag="PK264_08830"
     CDS             complement(<2082..3382)
                     /locus_tag="PK264_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HRD76031.1"
                     /translation="MKSVTVHLPALQVVVPLVGALLSAWFRRGTAAWAFALLVSWLMP
                     LISVALLLQVLQSGPISYHLGGWAPPWGIEYRVDLLNAFVLVLVSVVGAAIMPFARRS
                     VAHEVEGDKQAWFYTMYLLCLTGLLGITITGDAFNVFVFLEISSLSTYVLIAMGKDRR
                     ALLAAYQYLILGTIGATFYIIGVGFLYLVTGSLNIVDIAQRLGPAASAAPRPVMAALA
                     FMAVGVSLKLALFPLHVWLPGAYAHAPSVATAFLAGTATKVAIYVLIRFFFSVFGVAI
                     SFKALPVAEVLLVLSVAAMFIASLVAVFETDTKRMLAYSSLAQIGYITLGISLANASG
                     LTGGVVHLFNHALMKTALFLALGAVAYRIGSVKLADLAGIGRQMPLTMAAFVVGGLAI
                     IGVPGTAGFVSKWYLAVGAFERGWWPLVFLLLASSLLAVVY"
     gene            complement(3379..3753)
                     /locus_tag="PK264_08835"
     CDS             complement(3379..3753)
                     /locus_tag="PK264_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HRD76032.1"
                     /translation="MQIPAAIQRIAEHYNYVVTIFLMVAGLYIVIARGNMIKKLIGLS
                     LFQTSVYLLYISPGKIIGGTAPILDPAFKVYSNPLPHVLILTAIVVGVATLALGLALV
                     ARIQEAYGTIEEDELFGQEGDQ"
     gene            complement(3753..4169)
                     /locus_tag="PK264_08840"
     CDS             complement(3753..4169)
                     /locus_tag="PK264_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HRD76033.1"
                     /translation="MKRDLILRVIAKLTLPFMLLFALYVQFHGELGPGGGFQAGVIAA
                     GMVILHALIFGIREAKRIAPQALVERLVPLGVLIYAGTGIVGLLTGVNFLDYSRLGPD
                     SPHGHEYGIFAVEVGVLVTVSSTMIAIFYAFVERGR"
     gene            complement(4166..4720)
                     /locus_tag="PK264_08845"
     CDS             complement(4166..4720)
                     /locus_tag="PK264_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRD76034.1"
                     /translation="MALLTLLAVVTVGIARQRNLFGAIILAGIYSFLMASLLVVLDAV
                     DVAMTEASVGAGVSTVFLLAALHLARSMEQKPHHSPVLPMVVAVGVGAALFWGTVGLA
                     PFGTPDNVIHKHVAPKYLADSLKETMVPNVVTSTLADYRGYDTLGETTVIFTAGVGVM
                     LLLKGRRRRKAEAQSAKARSEAAQ"
     gene            complement(4751..5116)
                     /gene="mnhG"
                     /locus_tag="PK264_08850"
     CDS             complement(4751..5116)
                     /gene="mnhG"
                     /locus_tag="PK264_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HRD76035.1"
                     /translation="MASWALLLGGTFFTVAGMIGLVRMPDLFTRLHAASVTDTLGAGL
                     LCGGLMLQAGPTLVAVKLLFILALLFFTGPAITHALAQAALHAGVEPQLSEDRRDRPD
                     AAAIEVERTSDASSFAPAV"
     gene            complement(5134..5412)
                     /locus_tag="PK264_08855"
     CDS             complement(5134..5412)
                     /locus_tag="PK264_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HRD76036.1"
                     /translation="MMFAAAALGILVALALAVTRALKGPTVFDRVLAANSIGTLAILL
                     LAVAGFLTGRPEWLDIAITYGLLNLIGTLAILKFFRHGDLAYDVEEER"
     gene            complement(5409..5882)
                     /locus_tag="PK264_08860"
     CDS             complement(5409..5882)
                     /locus_tag="PK264_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HRD76037.1"
                     /translation="MRIVSLFIALLLFWLALSGYFKPFLVGAGVASSLVCIWVARRMG
                     AADGEGHPHHLFGRALTYFPWLVWEIVKSCWAVTKVILHPRLPISPTMTRVAASQRSS
                     VGLVTYANSITLTPGTITTAVDGNILTVHALVRDGAIDLEGGGMDARVTRFEGAR"
     gene            complement(5870..6106)
                     /locus_tag="PK264_08865"
     CDS             complement(5870..6106)
                     /locus_tag="PK264_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76038.1"
                     /translation="MLITDRLLPPWPGLDTIFGLKQSISGLTIAYLSDGAPDNRRLGL
                     QAGADLILPNPLRRAHVLEAVAHADPAGRLECAS"
     gene            complement(6259..6588)
                     /locus_tag="PK264_08870"
     CDS             complement(6259..6588)
                     /locus_tag="PK264_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD76039.1"
                     /translation="MVRSRGILKENVIRQLSSDAEARLQPLLGQLASAGVEARTEVQL
                     SEEPQAILDVARRHGCNSIVVLGRPIVGARRRWLSATGCVGHHLGARLAALSDTPVTV
                     LPIDAGI"
     gene            6943..>7884
                     /locus_tag="PK264_08875"
     CDS             6943..>7884
                     /locus_tag="PK264_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HRD76040.1"
                     /translation="MAFPRLLSLPPHVIGFGYLLAYIALDWISFVHPYGPFAITPWNP
                     STGLSFVLVLLFGLRHLPMLFLAPVAADLLIRGVPAGFAGVLGGLVIGGGYSVAAFLL
                     LRPLPRFDVSLSSTRDLFLLLLVAAGSSSLVAVAYISIHVIFGQLPMSAFSPAILRFW
                     IGDLIGIAVVTPFLLILLTRGALFDLGWDTAMQVLLLLFCLLLAFGTAQGRQLQLFYL
                     LFLPIIWIAIRAGLEGVTAGLVLTQLGLIVALQMQSPTAVDVTTFQALMLILTLTGLA
                     AGALVTERRRAELQLRLQQDAQARLTRLGSMGELASAL"
     assembly_gap    7885..8077
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8530..9147
                     /locus_tag="PK264_08880"
     CDS             8530..9147
                     /locus_tag="PK264_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD76041.1"
                     /translation="MTSGITVHLIDDDTAILDSLGLYLESKGFTVERHSRAQPFARLA
                     ASTLDQACIVSDVRMPGMSGIDLQQHLNQRGVTAPLILITGHGDIDMAVAAIKAGAHD
                     FLEKPFDERRLVASIKAAMAAHAGRQQQSQALADLRASYSELSERQQQVMDLAVKGRT
                     NKEIGATLGISPRTVEIYRAKVMERMGAYTLADLVRIALVIAERH"
     gene            9430..>9690
                     /locus_tag="PK264_08885"
     CDS             9430..>9690
                     /locus_tag="PK264_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HRD76042.1"
                     /translation="MQVERRLRDLALFNPAIDSKLRGCDVVAVRVDDVAPNGYTMDRA
                     TVRQKKTGRPVKYERTEQTRQAIDVHLRESGRKSDQFLFAGRG"
     assembly_gap    9691..9700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9701..9960
                     /locus_tag="PK264_08890"
     CDS             <9701..9960
                     /locus_tag="PK264_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD76043.1"
                     /translation="GEPYFGLTVRQYARLVNEWISCIGLDPLKFGTHTLRRTKATLIY
                     RQTGDLRAVQLLLGYTKIESTVRYLGIEIDDANEISEKIDI"
     gene            complement(10257..11039)
                     /locus_tag="PK264_08895"
     CDS             complement(10257..11039)
                     /locus_tag="PK264_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HRD76044.1"
                     /translation="MSTGHHYWCGAAGHENKREATMTETNAISLPLSGNGFAFVEGTR
                     MRAILEAVGPLGDWAEFAASWGNLETDTYMADGGRYRRRRHAVFSVAASGLATREAHQ
                     PHYQGRDYNALNGGIDRWFAPLEPQIGASKSLASILAFTARCFGRLAPETRTWKVEIH
                     QFRIEAKAGTEGRPTPEGMHRDGVDFVLVLLVNRTNIVSGTTTIADLDKRPLGSFTLA
                     APFDAAIVDDRCVYHGVTPVVPDDPSKPAYRDVLVVTFRAVG"
     assembly_gap    11148..11160
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11161..12548
                     /locus_tag="PK264_08900"
     CDS             <11161..12548
                     /locus_tag="PK264_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRD76045.1"
                     /translation="PLNTQLGRQLRALILDGRISAGARLPSTRSLADDLGVSRATVVL
                     AFEQLVSEGYIEGRRGSGMYVADDLPEQALQVRAPTAAKSAATRMPPVPMPGPHRPFQ
                     PGASDGQLFPHQEWSRLLYRSWRSPPSALLGTIDPFGWPPLREAIARHLHEWRGIACG
                     AHQILITGGTADATELVAHTAFAPGAVLHIEDPGYPTLRYTLESLGFRVYPVPVDDDG
                     FDIARAAALGTAAGAIIAPSRQFPLGATLPVGRRLQLLDWASAEHAFLVEDDFDSEYR
                     YLGSPLPALMSLDRHGRTIYIGSFSKVLSPALRLGFVVLPEALIERARRHLAERGTLA
                     SLVAQPALAEFIASGQYATHIRRTRRIYARRMQALMASAPRLAGLLDLKPTSAGMHIV
                     AELDPRLAARKSDRDLSAALAAAGIIAPPLAAYYAGAATRAGLLLGFAGFDEAAITTA
                     AETMSRVLADL"
     gene            complement(12560..12931)
                     /locus_tag="PK264_08905"
     CDS             complement(12560..12931)
                     /locus_tag="PK264_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76046.1"
                     /translation="MALRRVRLDLARDHDHPNGSDRHGYDFIAPLDDQGHLAASEWRE
                     HRERCRVKRFWAGEKDMLGRLVHKRSRAWVFDYNPKSDSDDETGFKFDLHRFVPGEYV
                     SITEHDGVQRTFHVRSVVDLD"
     gene            complement(12963..>13921)
                     /locus_tag="PK264_08910"
     CDS             complement(12963..>13921)
                     /locus_tag="PK264_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD76047.1"
                     /translation="GRCSVEPQLLVAHPGCGRHAGATSGRPPQDLLIEKDVAMALKTI
                     LLHLTDPGRATRLIAAAVPLARAAGAHLVGLSVLPPYIVMPAADGGGTSITMDEHRIS
                     YMADMARMKRAFLEATADLPRPSEWREADAAFSSVSDAVLDHVRAADLVIASQPDPKW
                     GGSPLTEDPVRLVMEGGRPVLLIPARGDVRMPPSRVAVAWDGRREAARAAFDAIPLAV
                     LAGNVTIISVNGDRAGRSAGDLPTAEIAAALARHGAKADSVDATARGGDVGHELLRQA
                     HAHGADLLVMGCYGHSRLRELVLGGATRDVLAGTDMPILMSH"
     assembly_gap    13922..14180
                     /estimated_length=259
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14181..15221)
                     /locus_tag="PK264_08915"
     CDS             complement(<14181..15221)
                     /locus_tag="PK264_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRD76048.1"
                     /translation="MAASDAEVAVEAGGGPHRGLARRVTLPLAILFGLGVTIGAGIYV
                     LIGAAAGRAGMHAPLAFVLAAIVMVPTAAAFAEFATRMPVSAGEAAYARAGFNSQALA
                     LLVGLMVIAVGILSAAAISRGSSGYIRELTGLPLALIVPLVVLAMGAITAIGIKESVG
                     VAAVMTVIEIGGLLAIIVLGGSGNGQVVTRLPEIWTGLTNLPVLLGILSATLLAFFAF
                     IGFEGLANIAEEVEAPERTLPLAIFWTLVLSTILYIAVVWVALIAVPRDELAAAEAPL
                     SLVFKRVTSAPPVLITTIAIVATVNGIIAQMIMASRVVYGLADRGQLPKALASVHLAS
                     RSPRNAIAMVVVL"
     gene            complement(15237..>15761)
                     /locus_tag="PK264_08920"
     CDS             complement(15237..>15761)
                     /locus_tag="PK264_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD76049.1"
                     /translation="ARTADLVIAGQRDPDYDFSIVLDVPERLAVETGRPVLVIPRVGD
                     FPTIGSRVTVAWNGGREAARATFDALPLLQLAQSVRVVSVSQHDDGTVGRSLPTTEIA
                     TTLARHGIRVEAAMTTAENIKVGDALLSGLCDEQTDLLVMGAYGHSRFREMVFGGATQ
                     HILAHMTAPVLLSH"
     assembly_gap    15762..15808
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15809..16064)
                     /locus_tag="PK264_08925"
     CDS             complement(<15809..16064)
                     /locus_tag="PK264_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76050.1"
                     /translation="MSYKTILLHLNDEHKVARLVNAALQIAEKFEAHVTALYVLPPVP
                     TYGSMVMGSTIINSSMQGFRKEAERVKAAFDLACKGRTVVP"
     gene            complement(16152..16751)
                     /locus_tag="PK264_08930"
     CDS             complement(16152..16751)
                     /locus_tag="PK264_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76051.1"
                     /translation="MTLMPARGSIDGSGGPTDAVPRAWTRIAIGVYGEPEPLCRALGG
                     LHRFGAGALAIAGTETTLVRFQESKSPSRRVGAALIDGPRDVFGDIGAPTPALSGWIE
                     DWRALDELVAAAVPIGSSGTLATVRASPGIARLLAGMANQNAMTRDRAATDLSSHLVH
                     GRAALAIKAETAAELRQAVQALLATSQFPVETRAMISLG"
     gene            complement(16782..>17003)
                     /locus_tag="PK264_08935"
     CDS             complement(16782..>17003)
                     /locus_tag="PK264_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD76052.1"
                     /translation="RSARRGGSPREIVLPMTRSDIADFLGLTIETVSRTFTRLRAEGL
                     IEIEQCILVTIKDEAALEATALADAGRRH"
BASE COUNT         3069 a         5235 c         5445 g         2702 t
ORIGIN      
        1 gagccacaac gtcagaagag gcaggacgag aaacgcaacc gcgcgtgctg tgccgcgcag
       61 aagcggcaac gccagcgcgc caaggatcat gacgaaagcc ggcggcagca gcaggctagt
      121 ggtcatagta gctgtccgac cgcttgagga ggacgccgag cagcttggca aaaagcacca
      181 tggcgacgca ggcggcgaag ccgaaccatg cgccgaagcc gaacgtgccg tcgatgccga
      241 agtgcggctt gtgctcgacg acgaggtcga gtgcgacgag agccgccagc agcagtgagc
      301 cgctgatcca gagcagccga atggtggatg ggcgcaccag ccagtggtcg cgatcgggcg
      361 gcagcggagt cggcggctgt tcaggctccg tcatcgcgcg actcccgtga gcatgagttt
      421 ggcaagcgcc agcgggatgt ccggagcgag gaagaggacg atggttgcgc cagcagtgac
      481 cgtcagtgcg atcaccatcg gcaagggcgc ttcaccgtga tcgtcgtggt gcgtcgctgc
      541 ggcaccgacc gcatgtcctc gcgcgggttc cacgaagaac gcgcggaaca ggatcggcag
      601 aaagtaggag gcgttgagca cggtcgagag cacgatgacg ccgatcggca gccattgctg
      661 cgtcgcgaag gcccccgaca gcatgaacca cttgccgagg aagccggccg tcggcggcag
      721 gccgatcatg ccgagcgcac cgatggcaaa ggccgtcatg gtccaaggca tctgccagcc
      781 gatgccgtcg agttcggaca cctcggtctt gtgggccgcc gtgtagatgg cgccggccgc
      841 aaagaagagc gtgatcttgg cgacagcgtg cgccgcgatg tgcattgcgg cgccgattgc
      901 cgatatcggc gtcaggatcg caaccgcaag cacgacgtag gagagctgcg agacggtgga
      961 ataggcgagg cgcttcttca gattgtcctg ccgcagtgcc accatggagg caagtaccac
     1021 cgtgccgccc gcggcccaga cgagccacca cgcggcgccg tcggcagcga ggcgctctac
     1081 gccgaacacg tagacgataa ccttgagcac cgtgaacacg ccggccttga ccacggccac
     1141 ggcgtgcagg agtgcggaga ccggcgtcgg tgcgaccatt gccgcgggta gccagaaatg
     1201 cagcggcatc agcgcagctt tgccgatgcc gaacacatag agcgcgagca gcgcgccgat
     1261 ggcgccagcg cccaggctgg ctggaaggat gccgcccgcc ttgaagtcca tcgtgccggc
     1321 gagaaagccc gtgccgacga tcgcgggcag cagcagcaac atcgaagttc cgatgagcag
     1381 catcaggtag atgcgaccac cacgcacggc ctcatcgctg gccttgtgcg tgacgagcgg
     1441 gtaggtcgac agcgtcagca gctcgtagaa caggaacagc gtggacaggt tcttggcaaa
     1501 cgccagaccg atggtggagc cgatggcgac ggcaaagcag acgtagaacg aggtctggcg
     1561 cggttcatcg ttcgcgcgca tgtagccgat cgagtagatc gagttcacga tccagagcga
     1621 cgaagcgacg agcgcgaaca acatgccgag gggctcgata ttgaaggcga tcgtgagtcc
     1681 agggacgatg tcaagcagcg tccgctccgg ctgttggccc gcgagcacgt gcggcagcag
     1741 ggaccacacc acaacggcga gtttagcagc cgtcgccagt gtcaccgctt cgcgcaggtt
     1801 cggccagcgg tggaacactg gaatgagcag cgccccgatg aacgggatga tcagcgcgag
     1861 tgccaacaag gtgtcgggcg accacgggag cctcatttga gccccccgag caggctcgct
     1921 gccacctttc gcgcgatgcc agccgacgcc tcggtgtcga taccgaagta gatcgtcgcg
     1981 gcagccagaa gcagtagcgg cgcaagcatc gacaatggcg gatcgctcgc cttggaggcc
     2041 atgggcgacg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natgtagacg acggcgagca
     2101 gcgagctggc gagaaggagg aacaccagcg gccaccagcc ccgctcgaag gcccccactg
     2161 cgaggtacca cttagagacg aagcccgccg tgcccggcac accgatgatg gcgaggccgc
     2221 cgacgacaaa cgcagccatc gtcagcggca tctgccgccc gatgcccgcg aggtcggcga
     2281 gttttacgct gccgatgcga taggccactg caccgagtgc gaggaacagc gccgtcttca
     2341 tcaacgcgtg attgaagaga tgcacaacgc cgccagtgag gcccgaggca ttggcgaggc
     2401 tgatgccgag tgtgatgtag ccgatctgcg ctagtgacga gtacgcgagc atgcgcttcg
     2461 tgtcagtctc gaacacagcc accagagagg cgatgaacat ggccgcgacg gaaagcacga
     2521 gcagcacttc cgcgaccggc aatgccttga agctgatggc aacgccgaag accgagaaga
     2581 agaatcggat cagcacgtag atcgcgacct tggtggcggt accggcgaga aacgccgtgg
     2641 caaccgacgg ggcgtgtgcg taggcgcctg gcagccagac gtgtagcggg aagagcgcaa
     2701 gtttcaggct gacgccgaca gccatgaagg cgagcgcggc catcacgggg cgtggtgctg
     2761 ccgaagcagc cggaccgagg cgttgcgcga tgtcgacgat attgaggctg ccggtgacaa
     2821 gatagaggaa gccgacgccg ataatgtaaa aggtcgcccc aatggtgccg aggatcaggt
     2881 actgataggc ggcgagcagc gcgcgccgat ccttgcccat tgcaatcagc acgtaggtcg
     2941 acagcgacga gatttccagg aacacgaaga cgttgaacgc atcgccggtg atggtgatgc
     3001 cgagcagacc cgtcaggcac agcagataca tggtgtagaa ccacgcctgc ttgtcgcctt
     3061 cgacctcgtg cgcgacgctg cggcgtgcaa acggcatgat cgctgcgccg acaaccgata
     3121 cgagtaccag tacgaacgca ttgagtagat cgacgcggta ttcgatgccc cagggcggcg
     3181 cccatccgcc gaggtgatag gagatcggtc ccgattgcag gacctgcaag agcagcgcga
     3241 ccgaaatcag cggcatcagc cacgagacga gcagcgcgaa cgcccaggcc gccgtgcctc
     3301 gccggaacca tgcggagagc agtgcgccga cgagaggcac gacgacctgg agcgcgggaa
     3361 ggtggactgt gacggacttc actggtcccc ctcctgtccg aagagttcat cctcctcaat
     3421 agtgccgtac gcctcctgga tgcgggcgac gagtgccagt cccagcgcaa gtgtcgccac
     3481 gccgacgacg atggctgtca gaatgagcac gtgcggaagc gggttcgagt agactttgaa
     3541 ggcagggtcg agaatcggcg cggtgccacc gatgatcttt cccggcgaga tgtagagcag
     3601 gtaaaccgaa gtctggaaca gcgacaggcc gatcagcttc ttgatcatgt tgccacgggc
     3661 gatgacgatg tagagcccgg ccaccatcag gaagatcgtg acgacgtagt tgtagtgctc
     3721 ggcgattcgc tggatcgcgg cgggaatttg catcagcggc cccgctctac gaaggcgtag
     3781 aagatcgcga tcattgtcga cgacaccgtg acgagcacgc cgacctcgac tgcgaagatg
     3841 ccgtactcgt gtccatgcgg gctgtcgggc ccaaggcgag agtagtcgag gaagttcacg
     3901 cccgtcagca ggccgacaat tccagtgcct gcgtagatca gcacgccgag cggtaccaga
     3961 cgctcgacaa gcgcctgcgg cgcgatccgc ttggcctcac gaatgccgaa gatcaacgca
     4021 tggaggatca ccatgccggc tgcaatgacg ccggcctgga agccgccacc gggaccaagc
     4081 tcgccatgga actgcacata gagtgcgaac agcagcatga atggcagcgt cagcttggcg
     4141 atgacgcgga ggatgaggtc gcgcttcatt gggcggcctc cgaacgcgct ttggctgatt
     4201 gagcctcggc tttgcgtcgc cgccggccct tgagcaacag catgacgccg acgccggctg
     4261 tgaagatcac cgtggtctcg ccaagcgtgt cgtagccgcg gtagtctgct agcgtcgagg
     4321 tgacgacgtt cggaaccatg gtttctttga gactgtcggc gaggtatttg ggcgccacat
     4381 gcttgtggat gacattgtcc ggtgtcccga aaggcgcaag gccgactgtc ccccagaaga
     4441 gcgcggcccc tacacccact gcgacaacca tcggcagaac cggagagtgg tgcggcttct
     4501 gctccatgct acgtgctaaa tgcagcgccg ccagcaggaa gactgtcgac accccagcac
     4561 caaccgaggc ttcagtcatc gccacgtcga ccgcatcgag cacgacgagc aggctcgcca
     4621 tcagaaagct gtatatgcca gcgaggatga tggcgccgaa gaggttgcgc tggcgtgcga
     4681 tgccgacggt gacgacggca agcaacgtca gcagtgccat gttgatcaga gcttccaagc
     4741 gactacctca ctacaccgcc ggtgcgaacg atgacgcgtc cgacgtgcgc tctacttcga
     4801 tcgcagccgc atccggtcga tcgcgccgat cttcggagag ttgaggttcg actccggcat
     4861 gcaaggccgc ctgggccagg gcatgggtga ttgctggccc tgtaaagaag agcagcgcca
     4921 agatgaagag cagcttcacg gccacgagcg tcggcccggc ctgcagcatg aggcctccac
     4981 aaagcaggcc ggcaccgagc gtatcggtga cgcttgctgc atgcagccgc gtgaacagat
     5041 ccggcatgcg cacgaggccg atcatgccgg caaccgtgaa gaaggtgccg ccgagcaaaa
     5101 gcgcccagct cgccacatcg acaataagcg ctctcaccgc tcctcctcca cgtcgtaagc
     5161 gaggtctccg tggcggaaga acttcaggat cgccagtgtg ccgatcagat tgagcagccc
     5221 gtaggtgatc gcgatgtcga gccactccgg ccggccggtc aagaaccccg cgactgccag
     5281 caggagaatg gcgagagtgc cgatcgagtt ggcggctagc acgcgatcga agacggtcgg
     5341 tcctttgagt gcgcgagtga ccgccagagc gagggcaacg agtatgccta gcgcagccgc
     5401 agcgaacatc atcgcgcccc ctcgaatcga gttacgcgcg catccatacc gccgccttcc
     5461 aggtctatgg caccgtctcg gaccagcgcg tggaccgtca gtatgttgcc atcgactgcc
     5521 gtcgttatag ttccgggcgt cagcgtgatc gagttggcgt aggtcacgag tccgacgctc
     5581 gaacgctggc tcgccgctac acgcgtcatg gtgggagaga tcggcaagcg tgggtgcagg
     5641 atgaccttgg tcacggccca gcacgatttc acaatctccc agacgagcca cgggaaatac
     5701 gtgagcgcac gaccgaacaa atgatggggg tggccctccc cgtcggcggc tcccatgcgc
     5761 cgcgccaccc agatgcacac gaggctgctc gccacgcccg caccgacgag aaacggcttg
     5821 aagtagccgg acagcgccag ccagaatagt agcagcgcga tgaacaggct cacgatgcgc
     5881 attcgagcct ccccgccgga tcggcgtgcg ccacggcttc gagtacgtgc gcacggcgga
     5941 gtgggttcgg cagtatcagg tcggcgccgg cctgcagccc gagccgccgg ttgtcaggtg
     6001 cgccgtcgct caagtaagca attgtaagtc cggaaatcga ttgcttcaga ccaaagatcg
     6061 tatccagccc tggccagggc ggcagcaagc ggtcggtgat cagcaacttg gtcactccct
     6121 cggcgattgt ccgacgagcg gcatagcctg atcctgcaat cgacgggcga tgcccggagg
     6181 tcgtcagcca ataggcgatg cgttcgcaca catgctcgtc actggagagg acaacaacat
     6241 tgggaggccg ctggatcatc agatgcccgc atcgatcggg agaacggtga cgggtgtgtc
     6301 ggagagcgcg gcgaggcgag cgccgaggtg gtggccgacg caaccggtcg ccgacaacca
     6361 tcgacggcgt gcgccaacga tcgggcggcc aagcactaca atcgaattgc agccatgcct
     6421 tctcgccaca tcgagaatcg cctgcggctc ctccgacagt tggacctcgg ttcgagcctc
     6481 cacgccggcc gacgccagct gtccgagcag cggttgcaac cgggcttcag cgtcggacga
     6541 gagctgtcgg atcacgttct ccttcagaat gccgcgcgag cgcaccatct ccggctcggg
     6601 ctgcacggca agcaagacaa cgcgagcgag tgatccgatc gtactcagat ccgctacgtc
     6661 gacttcagcg gcctctctcg cgagcacgat catcactggg gatgccgaag cgagcttagg
     6721 aatgggttga gcttccgcat gtgtcatggc gaggcatccg tcgaggccgt aggattgtcg
     6781 tcagaaaagt cggcggaagc gtcgacgcgc aattcaggtc ggcaccgtaa ggtgctaccc
     6841 tctcagggaa ccaccttatg gtgaggccct taggtgccga gacctgccga gacagctaca
     6901 gcaacggagt atgagcgcgc gacccgagcc aaagtccgcc cgatggcctt tccacgactg
     6961 ctgtcgcttc caccgcacgt catcggcttc ggctatctcc tggcctacat cgctttggac
     7021 tggatcagct tcgtgcatcc ctatgggccg ttcgcaatca cgccctggaa cccgtcgacc
     7081 ggattgagct tcgttctcgt gctgctgttc ggcctccggc acctgccgat gctgtttctc
     7141 gcaccggtcg cggcagacct tttgatacgt ggtgtccccg ctggttttgc gggcgttctc
     7201 ggcgggctcg tgatcggcgg cggctattca gttgcagcat tcttgctgct gcgcccgttg
     7261 ccgcgcttcg acgtgtccct gtcgtcgacg cgcgatctgt ttttgctctt gctcgtcgct
     7321 gctggcagtt cctcgctcgt tgctgtcgcc tacatttcga tccatgtcat tttcggccag
     7381 ctcccaatgt ccgccttcag cccggccatc ctgcgcttct ggatcggcga tctgattggg
     7441 atcgcagtcg taaccccgtt tctgctcatc cttttgacac ggggggcgtt gttcgatctc
     7501 ggttgggata cggcgatgca ggtcctgttg ctgttgttct gcctgctgct tgctttcgga
     7561 acggcgcaag gccgacagct gcagctgttc tacctgctct tcctccccat catctggatc
     7621 gccatccgag cagggctcga aggcgtgacc gcgggtttgg ttctcacgca gcttggtctc
     7681 attgtggcgc tgcagatgca gtcgccgacc gccgtcgatg tcacgacctt ccaggcgctg
     7741 atgctgatcc ttacgctcac ggggctcgct gctggtgccc tggtgacgga gcgtcgccgc
     7801 gcagagctac agcttcgcct gcagcaggac gctcaagcac ggctgacacg tctcggcagc
     7861 atgggagagc tggcctcggc attgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncca gccagtgtgt cgcgcatcat
     8101 cgacgatgcc gtcgagctga tccaacccga gatcgaacgc agcggcatcc gggtcgatcg
     8161 tcggctgcaa ggagatctgc cagcggtcat ggccgatgcg ctgcaggccg agcaagtgct
     8221 cctgaacctc gtgcgcaatg cgatagaggc gttccatgag gccagtcgcg atcgtggcct
     8281 catcacgatc gaggctcgac gcatcggggg acatgtcgag ctgagcgttg ccgacgacgg
     8341 tccgggcttc gcaagtatcg atgacgcctc tggtccgacc gcgctcactt ccacaaagcc
     8401 ggatggcctc ggcatcggac tcgccctttg tcgctcgatc atccaggcgc acggaggttc
     8461 actggagatt tcaaataccg gcaagggcgc gctcgtgcga tttaccctgc cagtgaccga
     8521 gggcggcaca tgacgagtgg aatcacagtc cacctcatcg acgatgacac ggcgatcctc
     8581 gattcgctcg ggctctatct cgaatccaag ggattcaccg tcgaacgcca cagccgcgcc
     8641 cagccgttcg cccggctcgc tgcgtccacg ttggatcaag cctgcatcgt cagtgacgtc
     8701 cggatgcccg gaatgtcggg aatcgatctg cagcagcacc tcaatcagcg aggcgtcacc
     8761 gcgcccctca tcctgatcac ggggcacggc gacatcgaca tggcagtggc agcgattaag
     8821 gccggcgccc acgacttttt ggagaagccg ttcgatgaac gccgactggt tgcctcgatc
     8881 aaggcggcga tggccgctca tgcaggtcgc caacaacaat cccaggcatt ggctgatcta
     8941 cgtgcgagct acagcgaatt gtcagaacgt cagcagcagg tcatggacct cgccgtgaag
     9001 ggccgcacca acaaggaaat cggcgccacg ctcggcatca gcccacgcac ggtggaaatc
     9061 tatcgcgcca aggtcatgga acgcatgggt gcctacaccc ttgccgacct cgtacgtatc
     9121 gccctggtga tcgcggagag acattgagca acggcatcct ttattccggc tcgcatctga
     9181 gccaccgaca agttcgtcga ggggcgtttg acagtcccgg agcgtctatt gagtttcttg
     9241 aaattacctg cctggacttc tgatctagtc gcaatttcgc aggtctcttt ggcatggaga
     9301 tcaagcatga acgacaatca agaaaaccca tccggaagac cgccccgccg tccgtcctgg
     9361 aacaagggca agctcgttgg cccgagaccg cagcttcgcc caagccatgt ctggtcgatc
     9421 cgcacatagc tgcaagtcga acggcgatta cgcgatctcg cattgttcaa tcccgccatc
     9481 gacagcaagc tgcgcggctg cgacgtggtc gccgtccgcg tcgatgacgt tgctccgaat
     9541 ggctacacga tggaccgcgc cactgtacga cagaagaaga cgggccgccc agtcaagtac
     9601 gagcggaccg agcagacacg gcaggccatc gacgtgcacc tacgagagag tggtcgcaag
     9661 tctgatcagt tcctgtttgc cggtcgcggc nnnnnnnnnn ggggggagcc atattttggg
     9721 ctgaccgtgc ggcaatacgc gcggctggtg aacgagtgga tctcgtgcat tggcctcgat
     9781 ccgctgaagt tcggcacaca cacgctgcgc cgcaccaaag cgacgctgat ctaccggcag
     9841 accggagacc tgcgcgcagt gcagctgttg ttggggtaca cgaagatcga aagcaccgtc
     9901 cgctatctcg gcatcgagat cgatgacgcc aacgagatct ccgagaagat cgacatttga
     9961 tcgagttggg cggcggctcg ccgccgtccg gccgagcgct cgatgcccgc tcctgaaaat
    10021 aacgaccgtc acagcgaccg aaaagaaccg tacggggttg ggtcacttgc aggcatcgag
    10081 gtcccactga attcggcaag gtcaggccag cttcagagat catcggcagg gcctgccagc
    10141 tcgttcaatg tcgcgtcgcc gtatggctgc tcctgcttgg ccgtgtcggt cttgggcgca
    10201 gcacggtctt gggcgcgaac ataggcccgc gcccccccca tgccgccaac gctctctcag
    10261 cccaccgccc tgaatgtcac caccagcaca tccctgtaag ccggcttcga cggatcatcc
    10321 ggcaccacgg gtgtcacgcc gtgatagacg caacggtcgt cgacgatcgc cgcatcgaac
    10381 ggcgcggcca gggtgaaact gccgagcggg cgcttgtcga ggtcggcaat ggtcgtcgtg
    10441 ccgctaacga tgttggtgcg gttgaccagc aggacgagca cgaagtcgac gccgtcgcgg
    10501 tgcatgccct cgggcgtcgg gcggccttcc gtgccggcct tggcttcgat gcggaactgg
    10561 tggatctcga ccttccaggt gcgcgtctcg ggcgcaaggc ggccgaagca gcgcgcggtg
    10621 aaggcgagga tgctcgcgag gctcttgctc gccccgatct gcggctcgag cggcgcaaac
    10681 cagcggtcga tgcctccgtt cagcgcattg taatcgcggc cctggtagtg gggctggtga
    10741 gcctcgcgtg tcgcaagacc actcgcggcc acactgaaca cggcgtggcg ccggcgccga
    10801 tagcgtccgc cgtcggccat gtaggtgtcg gtttcgaggt tgccccagct cgccgcgaac
    10861 tcggcccagt cgccgagcgg ccccacggcc tccaggatgg cgcgcatgcg cgtcccctcg
    10921 acgaacgcga agccgttgcc cgacagcggc aggctgatgg cgttggtctc ggtcatggtc
    10981 gcctcgcgct tgttctcgtg tccggccgca ccacaccaat agtggtgccc ggttgacatg
    11041 acggctcaat atcagtcaac tggtcggaga ataagaacca gtttacaaag atatgaccag
    11101 accacccatc aaagacgggg cggcagtcgc gctctcgctg cacctcannn nnnnnnnnnn
    11161 agccgctcaa cacgcaactc ggccggcagc tgcgcgcgct catcctcgac gggcgcatct
    11221 ccgccggcgc acggctcccc tcgacgcgat ctctcgccga tgacctcggc gtctcgcggg
    11281 cgacggtcgt cctcgccttc gagcagctcg ttagcgaggg ctacatcgag ggccggcgtg
    11341 gctcgggcat gtacgtcgcc gacgatcttc ccgagcaggc gctgcaggtg agggctccga
    11401 cggcggccaa gtcggcagcg acgcgcatgc cgccggtgcc gatgccgggg ccgcaccggc
    11461 ccttccagcc gggggcgagc gacggacagc tctttccaca tcaggagtgg tcgcggcttc
    11521 tctatcgtag ttggcgctcg ccaccttcgg ccctactcgg cactatcgat ccctttggct
    11581 ggccaccgtt gcgggaggcg atcgcccgcc atctgcatga atggcggggt atcgcctgcg
    11641 gggcccacca gatcctgatc acggggggca cggcggacgc gacggagctc gtggcccata
    11701 cggccttcgc gccgggcgcg gtcctgcata tcgaggatcc gggctacccg accctacgct
    11761 acacgctcga gagcctcggt ttcagagttt acccggtgcc ggtcgacgac gacggcttcg
    11821 acatcgcgcg tgccgctgcg ctcgggacgg cggcaggagc gatcattgcg ccctcacggc
    11881 agtttccgct cggggcgacg ctgcccgtcg gacgtcggct ccagttgctc gactgggcgt
    11941 cggcggagca cgccttcctg gtcgaggacg acttcgacag cgagtatcgc tacctgggtt
    12001 cgcccctgcc ggccctcatg agcctcgacc ggcacgggcg gacaatctac atcggcagct
    12061 tctcgaaagt tctgtcgccg gcgttgcggc tcggtttcgt ggtgctgccg gaggccctga
    12121 tcgagcgggc gcggcgacac ctcgccgagc gtggtacgct cgcctcgctg gttgcccagc
    12181 cggccctcgc cgaattcatc gcgagcgggc agtatgcgac ccatatccgc cgtacgcggc
    12241 gcatctatgc gcggcgcatg caggccctga tggcgtccgc gccgcgccta gccgggctgc
    12301 tcgacctgaa gccgacgagt gcgggcatgc acatcgtcgc cgagctcgac ccgaggcttg
    12361 cagcccgaaa gagcgaccgc gatctctccg cggcgctcgc ggcagccggc atcattgcgc
    12421 cgccgctggc cgcctactac gcgggcgcgg ccacacgcgc cgggctgctg ctcggctttg
    12481 ccggcttcga cgaggcggcg atcacgaccg ccgcggagac gatgagtcgg gtgcttgccg
    12541 atctatgacg ccggcaggat cagtcgaggt cgacgaccga gcggacgtgg aacgtccgct
    12601 gcacgccgtc gtgctcggtg atcgacacat attcgcccgg cacgaagcga tggagatcga
    12661 acttgaagcc cgtctcgtcg tccgagtctg actttggatt gtaatcgaac acccacgcgc
    12721 ggctacgctt gtggacgagg cggccgagca tgtccttctc gccggcccag aagcgcttga
    12781 cgcggcagcg ctcgcggtgc tctcgccatt cactcgcagc aaggtggccc tgatcatcga
    12841 gcggtgcgat gaaatcgtag ccatgcctgt cgctgccatt gggatgatcg tgatcgcggg
    12901 cgaggtcgag tcgaacgcgt ctgagagcca tggtgcgctc cttgggactt ggcctggtta
    12961 ggtcagtggg acatcaggat cggcatgtcc gtgccagcaa gcacgtcgcg cgtggcgccg
    13021 ccgagaacca gctcgcggag ccgcgaatga ccgtagcagc ccatgacgag aaggtcggcg
    13081 ccatgcgcgt gcgcctgccg caagagctcg tggccgacat ctccaccacg cgctgtcgcg
    13141 tccacgctgt cggccttggc cccgtgccgt gcgagggccg ctgcgatctc ggcggtcggc
    13201 agatcgccgg cggagcgacc ggccctgtcg ccgttcaccg agatgatcgt cacatttccg
    13261 gcgagcaccg ccagcgggat cgcatcgaat gcggcgcgcg ccgcctcgcg acggccgtcc
    13321 caggcgacgg cgacccgcga tggaggcatg cgcacgtcgc ctcgtgccgg gatgagtagg
    13381 acggggcgac cgccttccat gacgaggcgt acggggtcct cggtgagggg cgagcctccc
    13441 cacttcgggt ccggctggct ggcgatgacg agatcggccg ccctgacgtg gtcgagcacg
    13501 gcatccgaga cgctgctgaa ggcggcatcc gcctcgcgcc actcggaggg ccggggaagg
    13561 tccgcggtcg cctcgaggaa ggcccgcttc atgcgggcca tgtcggccat gtaggagatg
    13621 cggtgctcgt ccatggtgat gctggtgccg ccgccgtctg ccgccggcat gacgatgtag
    13681 gggggcagaa cggaaaggcc gacgaggtgg gcgccggcgg ctcgcgccaa gggtaccgcc
    13741 gccgcgatca ggcgggtcgc gcggcccgga tcggtgaggt gcaggaggat cgtcttcagc
    13801 gccatggcaa cgtccttctc gatgagcagg tcctggggcg gccggccgga cgtggcgccc
    13861 gcgtggcgcc cgcatcccgg gtgcgcgacc agcagttgag gctcgacgct gcagcgcccg
    13921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn caggacgacg accatggcga tcgcgttacg gggcgagcgg
    14221 ctcgcgaggt gcaccgacgc aagggccttc ggcagctggc cccggtcggc gaggccgtag
    14281 accacgcgcg atgccatgat catctgggcg atgatgccgt tcaccgtggc gacgatcgca
    14341 atggtggtga tcaggaccgg cggcgccgag gtgacgcgct tgaagacgag gctcaacggc
    14401 gcttcggccg cggcaagctc gtcgcggggg acggcgatga gcgcaaccca gacgacggcg
    14461 atatagagga ttgtcgagag gacgagcgtc cagaagatcg cgagcggcaa cgtgcgttcc
    14521 ggcgcctcga cctcctcggc gatattagcg agcccctcga agccgatgaa agcgaagaag
    14581 gcgaggagcg tcgccgagag gatcccgaga agaacaggca gattggtgag cccggtccag
    14641 atttccggga ggcgcgtgac cacctgcccg ttgccggagc ccccgagcac gatgatggca
    14701 agaaggccgc cgatctcgat gacggtcatc accgcggcga cgccgacgct ctccttgatg
    14761 cctatcgccg ttatcgcgcc catggcaagc acgacgagcg gcacgatcaa tgcgagcggc
    14821 aagcccgtga gctcgcggat gtagccggag ctgcccctgc tgattgccgc cgccgacagg
    14881 atgccgaccg ctatcaccat gaggccgacg agaagggcca gcgcctgcga gttgaagccg
    14941 gcgcgtgcat aggcggcctc acccgcgctg acgggcatgc gagtcgcgaa ttcggcgaag
    15001 gcggccgcag tcggcaccat aacgatcgcc gcgaggacga aggcgagcgg cgcatgcatg
    15061 cctgcccgtc cagccgcggc gcctatcagc acatagatgc ccgctccgat cgtcacgcca
    15121 agcccgaaca ggatggcgag cggcagcgtc accctcctcg ccagcccgcg atgcggccca
    15181 ccgccggcct cgaccgccac ctccgcatcg cttgcagcca tgggcgcctc ccgacttcaa
    15241 tgcgaaagca ataccggggc ggtcatatgg gcgaggatgt gctgcgtcgc gccgccgaac
    15301 accatctcgc ggaagcggga atggccgtaa gcgcccatca ccaggagatc ggtctgctcg
    15361 tcgcagaggc ccgagaggag ggcgtcgccg accttgatgt tctcggccgt agtcattgca
    15421 gcttccacgc ggatgccatg gcgggcaagc gtggttgcaa tctcggtcgt cggtaggctc
    15481 cggccaaccg ttccatcgtc gtgctggctg accgagacga cacgcacact ctgggcgagc
    15541 tggaggagag ggagggcgtc gaaggtcgcc cgcgctgcct cgcgaccgcc gttccaggca
    15601 accgtcacgc gcgagccgat cgtcgggaaa tcgccgacac gcgggatgac gaggacgggt
    15661 cgcccggtct cgaccgccag ccgctcgggc acatcgagaa cgatcgagaa gtcgtagtcg
    15721 gggtcgcgct ggccggcaat cacaaggtcg gcggtgcggg cnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggaaccacgg tgcgaccctt gcaggcaaga
    15841 tcgaaggcgg ccttgacgcg ctcggcctcc ttgcggaagc cctgcatcga gctgttgatg
    15901 atggtggagc ccatcaccat agagccatag gtcggcaccg gcggcagcac gtagagagcc
    15961 gtgacatggg cctcgaattt ctcggcgatc tggagtgccg cgttgacgag gcgcgccact
    16021 ttgtgctcgt cgttgagatg caggagaatg gtcttgtagg acatggttct tacctctggg
    16081 accgcttatt gagggacgtc accgcccgtc acgacatgga cggcgacagg tagcatcctc
    16141 gggtgatgtc gtcagccgag tgagatcatg gcacgcgtct ccacgggaaa ctgcgaggtt
    16201 gccagaagag cctggacggc ttggcgcagc tcagccgcgg tctccgcctt gatcgcgagt
    16261 gcggcgcgcc catggacgag atgactggag aggtcggtcg ccgccctgtc gcgcgtcatc
    16321 gcattttgat ttgccatgcc ggccaggagc cgcgcgatcc ccggactcgc tcggaccgtg
    16381 gcgagcgtgc ccgaactgcc gatgggcacc gccgccgcga cgagttcatc gagcgcgcgc
    16441 caatcctcga tccaacctga gagcgccggc gttggagcgc cgatgtcccc gaagacgtcc
    16501 cgtgggccat cgatcagggc cgctccgact cggcgcgacg ggctcttgga ttcctggaag
    16561 cggacgagcg tcgtctcagt gccggcaatg gcaagtgcgc cggcaccgaa cctgtggagt
    16621 ccgccgaggg cgcggcagag cggctccggc tccccataga caccgatcgc aatccgggtc
    16681 cacgcgcggg ggacggcgtc cgtcggtccg ccactgccgt cgattgaacc tcttgccggc
    16741 atcaatgtca tccgtgccgc cttgccttct gcccgctgcc gtcagtgccg ccgtccggcg
    16801 tcggcgaggg cggtcgcctc gagggcggcc tcgtccttga tggtgacgag gatgcactgc
    16861 tcgatctcga tgaggccctc ggcgcgcagc cgcgtgaagg tgcggctcac cgtctcgatg
    16921 gtgaggccga ggaagtcggc gatgtcgctc cgcgtcatcg gcagcacgat ctcgcgcggg
    16981 ctgccgccgc ggcgcgcgct gcg
//
LOCUS       DAORNH010000066        16919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2576_length_16919_cov_2.723850, whole genome shotgun sequence.
ACCESSION   DAORNH010000066 DAORNH010000000
VERSION     DAORNH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_16919_cov_2.723850"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            533..910
                     /locus_tag="PK264_08940"
     CDS             533..910
                     /locus_tag="PK264_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76053.1"
                     /translation="MQFRWISAALFPIALAAALGGAVAQSPTPAPPAPGGPQTPRFNA
                     TPGDGWSPPEIDCKCIYKGRAYSVGSVVCMDMPQGAVLTTCGMVLNNTSWQPSAEPCH
                     TSWRLRGLRFAVLQGRVALRCMR"
     gene            970..>1242
                     /locus_tag="PK264_08945"
     CDS             970..>1242
                     /locus_tag="PK264_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HRD76054.1"
                     /translation="MTSRWSARRQAFRAVTFGASCIHPASVHDPVSARIAADLGFEAG
                     MFAGSTASLTILGAPDLITITLTEFAEQAHRISRAAELPLMVDADHG"
     assembly_gap    1243..1272
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1273..1836
                     /locus_tag="PK264_08950"
     CDS             <1273..1836
                     /locus_tag="PK264_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRD76055.1"
                     /translation="LEAAGVSGLSIEDTELPRPYGPSDKQRLLSIEEGVGKMKAALAG
                     RRDPDLVIAGRTSAPQLTDIDDAIRRCRAYEAVGVDCIFLVGVKTRVELDKLAAALSK
                     PLILGTLTAELHDNAYLASRRVRVALLGHQPFQAAMQAIHQTMKALREGTPPGKLQGM
                     PPAELVRRITRQDEYDRATRDWLGGKA"
     gene            complement(1929..2282)
                     /gene="grxD"
                     /locus_tag="PK264_08955"
     CDS             complement(1929..2282)
                     /gene="grxD"
                     /locus_tag="PK264_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HRD76056.1"
                     /translation="MSEQQVHDWIRKTISANDVVLFMKGRKGMPQCGFSHQVDTILGH
                     LGVEYKDVNVLDDMGVREGIKSFTNWPTIPQLYIKGEFVGGCDIVREMFQQGELQALL
                     EEKGVAVSKPRTMTA"
     gene            complement(2398..2637)
                     /locus_tag="PK264_08960"
     CDS             complement(2398..2637)
                     /locus_tag="PK264_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HRD76057.1"
                     /translation="MPMDAADIERLIKARLPDATVEIRDLAGDGDHYAAHVVSPSFKG
                     KSRVMQHKMVYDALEGRMGGALHALALTTAAPKDG"
     gene            complement(2793..3995)
                     /locus_tag="PK264_08965"
     CDS             complement(2793..3995)
                     /locus_tag="PK264_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76058.1"
                     /translation="MPNKRNVGLLAVSQGLFQSVQSMGIATSPLAGYALLGEDKSLAT
                     LPIFLNHAGIMLTTIPASLLMDRIGRRAGFSIGAILGILFGGLAAYAVFVQSFTLLCV
                     ATLIQGSAAAFAWYYRFAAADAAPPDYKAKAISLVMAGGVIAGLVGPQLAKWSVNWFA
                     PVVFAGVYVTIALIGLLSLIVVQFVRIPRLTASERAEGGRPLSEIARSQTFLTALITS
                     MLGYGVMTLLMSSTPLAMQACGFKFEDSATVIQAHVVAMFLPSFFTGHLITRFGTLPI
                     IAAGAIIQTGCAIVNLMGIDFWNFLIANLLVGLGWNFTFVGGSALLTTTYRPAERARV
                     QAAHDFCVYSTTAIAAGLAGFLQQKAGWTVVNIAALPLMAVIVTAAMLLARRQRLEAG
                     LAAPTGKP"
     gene            complement(4168..4590)
                     /locus_tag="PK264_08970"
     CDS             complement(4168..4590)
                     /locus_tag="PK264_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76059.1"
                     /translation="MNGFDVPEALRALDPIYPMDLPAHPADLLAPTGVPPPPPTASVP
                     LARPPHLDLAPASRAAVGVVTFDLASQILEARDKLDKELSRASKEYQVLRSIAESELP
                     VCQDLVIELKQRLAGHALYEAILQLDAAYSTTLRYRRT"
     gene            complement(4790..5176)
                     /locus_tag="PK264_08975"
     CDS             complement(4790..5176)
                     /locus_tag="PK264_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76060.1"
                     /translation="MSTPADSRGAPLPASSGTSSDPFASPRLRATSEERRQRRAALFV
                     PELVLDTSSDNLVRQVRSLISSVEAALSASSKPYRLLKQIEDAGLSECARCTAELRNA
                     LERNHHFVTLIRLRDLEAALQRLDAA"
     gene            complement(5555..5980)
                     /locus_tag="PK264_08980"
     CDS             complement(5555..5980)
                     /locus_tag="PK264_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRD76061.1"
                     /translation="MTLRQRQRTLEIATRGVGLYEFTREAIEFVGGAELSTGLCTVFC
                     QHTSCSLLIQENADPDVQRDLQAFFRRLVPNGDPLFIHTAEGPDDMPAHVKAALTQSS
                     LSIPISAGRLALGTWQGIYLFEHRNAPHHRCVILHVVGD"
     gene            complement(5977..6261)
                     /locus_tag="PK264_08985"
     CDS             complement(5977..6261)
                     /locus_tag="PK264_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HRD76062.1"
                     /translation="MSFWKSLFGGGSPTQPAGPTAREEYKGYVIEARPYPEGGQFQVA
                     GTITREIGGERKEERFVRADRFATMEDAATVALNKARQIIDQNGDRLFRS"
     gene            6596..8233
                     /locus_tag="PK264_08990"
     CDS             6596..8233
                     /locus_tag="PK264_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76063.1"
                     /translation="MDRRSFLKSGLSVGAVAASGNLAAPAIAQKAAAKTLRFVPQANL
                     ANFDPIWGTQYVVRNASQLVWDTLYGIDDTLTAKPQMLEGDTVSSDGLTWTFKLRTGL
                     KFHDGAPVLAKDCVASIKRWSARDAMGQMLAKIQKELVAVDDRTFKWVLSKPFTKMRY
                     ALGKVGTPCCFIMPERFASIDPMKQITEFIGAGKPHAEFVGSGPMKFKIGDWVPGAKA
                     VFEKFDGYVPRSEPSKWFSGGKVMLVDRVEWVIMPDPATASAALQSGEVDWWESPISD
                     LIPVLKRNRNIKLDIADPLGNIGAFRLNHLHPPFKDVRVRRAVQMALSQEDYMRAVVG
                     NDTALWSTIPGFFTPGAPFYSELGGEPLKGPRNIDGAKKLLAEAGYSGLPVTCLVAQD
                     QPPLKAMGEITADLLKKIGMAVDFVATDWGTVGQRRASKNEPGKGGWGMFHTWHAGAD
                     CANPAAHIAVRAGGEKGPAWFGWPEDPAVEKAVDEWFEAKSVEDEKAAIARVNKAAME
                     SVVFIPTGHYKSYQAWRSNIEGVVNGPLPWFWGVKKS"
     assembly_gap    8373..8403
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8440..9381
                     /locus_tag="PK264_08995"
     CDS             8440..9381
                     /locus_tag="PK264_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76064.1"
                     /translation="MLTYILRRVLATIPVMAIVAFLVFSLLYIAPGDPAAVLAGEQAS
                     PEDVEKMRKSLGLDRPFLVRFVEWSAQILQGNFGTSLFTGLPVTTMIGQRIGPTLSLM
                     IVTLVLAVLIAVPMGVIAAWKAGTLLDRVLMAFAVVGFSVPVFVVGYLLAFYLGVEWD
                     WLPVQGYRELSEGFWPWLQHLTLPAIALGFVYMALIARITRATMLEVLSQDYVRTARA
                     KGVGQASILFLHALKNAAVPIVTVIGIGIAFLIGGAVVTESVFAIPGLGRLTIDSISR
                     RDYPTIQGIVLLFSFIYVLVNLAIDIIYTLLDPRIRY"
     gene            9378..10292
                     /locus_tag="PK264_09000"
     CDS             9378..10292
                     /locus_tag="PK264_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76065.1"
                     /translation="MSSDASPIAAPAAAPALPDIFPPVRPRTGVWGVVYRHPTMVIGG
                     AMLSLLAIFALFAPFLGTVDIDARELKLRNADPSAANWFGRDALGRDIYTRVVYGTRI
                     SLIVGVSVAILASLVGLSIGLVSGFVRWSDGIVMRIMDGLMSIPPILLAIALMALTGA
                     SVKNVIIAITIAEIPRVSRLVRGVVLSLREQPYVDAAIAAGTSTPMVILRHILPNTLA
                     PITVQATYICASAMITEAILSFVGAGIPAAMAPSWGNIMADGRSAWQAYPHVIFFPAI
                     FLSLTVLAVNLLGDGLRDALDPRMAKRI"
     gene            10296..11285
                     /locus_tag="PK264_09005"
     CDS             10296..11285
                     /locus_tag="PK264_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76066.1"
                     /translation="MALLEVENLQTHFRTIDGVNRAVDGVSFTLEAGETLAIVGESGC
                     GKSVTAMSLMRLIAMPPGRIAGRVSFAGRDLLAASEREMRQIRGNEISMIFQEPMTSL
                     NPVLTIGRQLGETLRLHQGLGKTEATARAVEMLRLVGIPEAERRVTEYPHQMSGGMRQ
                     RVMIAIALACNPKLLIADEPTTALDVTIQAQILDLMRDLKRRIGAAIILITHDLGVVA
                     EVAERVMVMYAGRKVEEAPVRALFHAPRHPYTRGLLGSVPKLGSSVTGSEERLAEIPG
                     LVPSLKSRIEGCVFASRCPIATEVCREVAPALEAKAARHVVACHHAAREAVTA"
     gene            11282..>12212
                     /locus_tag="PK264_09010"
     CDS             11282..>12212
                     /locus_tag="PK264_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD76067.1"
                     /translation="MSAPLLEVRNLKKHFPLTASLLGRSRGFVYAVDGVSFEVAKGET
                     LALVGESGCGKSTVGRAILRLFDITSGEVLIDGQRIDELSSGNLRPIRRRVQVVFQDP
                     FSSLNPRMRVRDILAEPIRNFGLAKDEADLDRRVGDLMDKVRLPREAVGRWPHEFSGG
                     QRQRIGIARALAAEPDLIICDEAVSALDVSVKAQIVNLLQDLQRELGLALLFISHDLA
                     IVEHITHRVAVMYLGKIVEVAPKRSIFAGPKHPYTEALLSAVPVPDPDAPRNRILLKG
                     DVPSPINPPKGCRFHTRCPKAFDRCRVEEPALKP"
     assembly_gap    12213..12347
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12560..15523
                     /locus_tag="PK264_09015"
     CDS             12560..15523
                     /locus_tag="PK264_09015"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRD76068.1"
                     /translation="MTRQPENDELARTSFLNGVNAAYIEELQARYDQNPGSVPDEWRL
                     FFQSLKEGPGAASAEREGPSWGRPISEMQQNGELVAALTGDYAPVERELRDRISHRAQ
                     SAGVEVSPAATFRATQDSLRALMLIRAYRVMGHLVADLDPLGISERKVHKELRPETYG
                     FTEADLDRPIFIDKVLGLETATVRQILKILRRTYCRHIGVEFMHITSPAQKRWIQERI
                     EGADKDVTFTREGKRAILNKLIAAEGFEKFSDIKYTGTKRFGLDGGESMIPALEQIIK
                     RGGQLGVKEIVIGMAHRGRLNVLANVMSKPLRAIFNEFKGGSYKPEEVEGSGDVKYHL
                     GASSDREFDGNKVHLSLTANPSHLEIVDPVVLGKVRAKQDQHRCEPGERTPVLPLLIH
                     GDAAFAGQGVVAECLGLSGLKGHRTGGSIHFIINNQIGFTTNPRYSRSSPYCTDVAKM
                     VEAPIFHVNGDNPEAVVHVAKVATEFRQKFQKPVVIDMVCYRRFGHNESDEPAFTQPI
                     MYRKIKAHQTVVSMYSRRLVEEGVMSEADVEGMQTSLRAHFDGELAAAEGYKPNKADW
                     LDGRWSQIGLPEDEARRGNTGLSIETLKDVGRRITTVPHDFQTHKTIGRLLERRREMI
                     ETGEGIDWAFAEALAFGTLVKERFAVRLSGQDVERGTFSQRHAVLIDQESERRYTPLK
                     YVAPDQARFEVINSMLSEEAVLGFEYGYSLAEPMALVMWEAQFGDFANGAQVVFDQFL
                     SSGERKWLRMSGLVCLLPHGYEGQGPEHSSARLERFLQLCAEDNWQVANCTTPSNYFH
                     ILRRQLQRKVRKPLILMTPKSLLRHKRVVSRLDEMGPGTSFHRVLWDDAQHLPGQKIK
                     LKPDGEIRRVVLCSGKVYYDLYEAREAAGIDDVYILRVEQLYPTPVRALIQELQRFPK
                     SDVVWCQEEPRNMGAWSFIEGQLEWVLAHTNGAVKRARYAGRAASASTATGLASKHAS
                     EQKALVADALGSAAA"
     gene            15520..16374
                     /locus_tag="PK264_09020"
     CDS             15520..16374
                     /locus_tag="PK264_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD76069.1"
                     /translation="MSDRTGKSDVARYYDFIYLWTQLTNRFGAFRVPQAHTIHRMLVD
                     PETGSFDGETIHRLIARHAKSLAPVRALDAGCGYGGTCMAMSKLDEGHWQGITINRTQ
                     VRVAARNAKALGLDGKVAIGHGSYDAPLSQTYNLIYGIESLVHSVDPSQTIANLAKAL
                     DPGGLFIIIDDMPVEDVPAAARADLEMFKASWRCPVMPTAAGWQKRLTGAGLEIAAVD
                     DLSPLSRPGTHEHIERSLAEVARRRRWMAPLGMSMVTDAQVGGLVLERLSRDGAVRYM
                     MIVARRPR"
     gene            16420..>16919
                     /locus_tag="PK264_09025"
     CDS             16420..>16919
                     /locus_tag="PK264_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRD76070.1"
                     /translation="MTIEIKVPALGESVTEATLSKWYKNAGDPVKADEPICELETDKV
                     TVEVPAPATGVMGEHKIGAGSTVAVGTVIGTLKPGAAGASVAAAAPAAVSPPKPAATP
                     AAPPPAAPSAMPPSPAARKILEEKGVASADVAGTGRRGQVMKSDAMAATAAPAPQPAA
                     ASLPVTR"
BASE COUNT         2835 a         5457 c         5562 g         2869 t
ORIGIN      
        1 gggaatcgac gctgtcgcat cgcccgagga accggcgcag cccgagagcg cggcagcgag
       61 cgccattgcg gagactgaaa gtagcgcgcg gtgcgcacag aagcggattg tcatctcaaa
      121 cccccaatgc attcagttcg gtacactctc ccgcacttgc actatattcc ctggcccttg
      181 tgccaggcct tgacatgtcg cacaccggcc atgaggccac ctcgacgagc acgggtccgc
      241 ttgcatcatc ccgcaatccg tcaggtcatt cgactcgtca gggcactccc cgtcgtatag
      301 gtcggccagt cggccttccc caaacagtat tggcctgatg gccccacttg ccagggccat
      361 gcctgtggaa acacccatat tgtggcaaat ttacaatggg ttgcccaagg gcctgccgac
      421 tccgacgatt aacagcctcc gcggtgatcc gctccggagc ggtcgattga agctgcttga
      481 cgactggtgg cagcagtctg ctcgtgctaa aagaagctag gaggcgtcgg ccatgcaatt
      541 ccgatggatc agtgccgcct tgttcccgat cgctctggcg gcagcgctgg gcggggcggt
      601 tgcccaatcc ccgacgccgg ctccaccagc cccggggggg ccgcagacgc cacgcttcaa
      661 tgctactccc ggcgatggct ggtcgccccc ggagatcgac tgcaagtgca tctataaggg
      721 ccgcgcctat tcggtcggta gcgtcgtttg catggacatg ccgcaggggg cggtgctgac
      781 cacctgcggc atggtcctga acaacacctc ctggcagccg agtgccgagc cctgccacac
      841 ctcctggcgc ctgcgcggtt tgcggtttgc cgtgctccaa ggcagagtgg cattgcgctg
      901 catgcgttga cagatgcgcc gagctatggc cgcttggggc cgtcgccacc atcggaccaa
      961 gagagccgca tgacatcccg ctggagtgct cgtcgccagg cgttccgcgc cgttaccttc
     1021 ggagcaagct gcattcatcc cgcatccgtg cacgatcccg tgtcggccag gatcgcggcc
     1081 gatcttggtt tcgaggccgg catgttcgcg ggctcgaccg cctcgctcac gatactgggc
     1141 gcgccggatc tcatcacgat cacgctcacc gagttcgccg agcaggcgca ccggatcagc
     1201 cgggcggccg agctcccgct gatggtcgat gccgatcacg ggnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnctcgaggc ggcgggtgtt tcgggcctct ccatcgagga caccgagctg
     1321 ccgcggccct acggcccctc cgacaagcag cgcctgctat cgatcgagga gggtgtgggc
     1381 aagatgaagg cggcgctggc cggccggcgt gatcctgatc tcgtcatcgc gggccgaacg
     1441 agtgccccgc agctcacaga catcgacgat gccatccgcc gctgccgcgc ctatgaggcg
     1501 gtgggcgtcg actgcatctt tctcgtcggc gtgaagacgc gggtcgagct cgacaagctt
     1561 gctgcggcgc tgtcgaagcc cctgatcctc ggcacgctca cggcggagtt gcacgacaac
     1621 gcctatctcg cctcgcggcg cgtgcgcgtg gccctccttg gccaccagcc attccaggcg
     1681 gcgatgcagg cgatccacca gacgatgaaa gcgctgcgcg aggggacgcc gcctggcaaa
     1741 ttgcagggca tgccgcccgc cgaactcgtg cggcgcatca cccgccagga cgagtatgat
     1801 cgcgcgacgc gcgactggct cggcggcaag gcgtgacggg cggcaagcgt gcgctcaaat
     1861 agaagaggcg cgacccccat ccggagatcg cgcccataga ttgaaagccg ctgcctgaac
     1921 tgcgctgatc aggccgtcat cgtgcgcggc ttggagacgg cgacgccctt ctcctcgagc
     1981 agcgcctgca gctcgccttg ctggaacatc tcgcgcacga tgtcgcagcc gccgacgaac
     2041 tcgcccttga tgtagagctg cgggatggtc ggccagttgg tgaaggactt gatgccctcg
     2101 cgcacgccca tgtcgtcgag cacgttgacg tccttgtact cgacgcctag gtgaccgagg
     2161 atggtgtcga cctggtgcga gaagccgcat tggggcatgc ccttgcggcc cttcatgaac
     2221 aggacgacgt cgttcgccga gatcgtcttg cggatccagt catgaacctg ctgctcgctc
     2281 atggtgctcc ccaggggatt ggtgcgccgt ccgctcaggc ggacgccgaa attacgcgcg
     2341 gaacatgtgg ttcctaccgg cgaaagtcaa tggcggccat tcgattgagg aaccggttca
     2401 gccatctttg ggggccgcag tcgtgagagc cagggcgtgg agcgcgcccc ccatgcgccc
     2461 ctcgagcgcg tcatagacca tcttgtgctg catgacccgc gacttgccct tgaacgaggg
     2521 gctcacgacg tgcgctgcat agtggtcgcc gtcgccggcg aggtcgcgga tctcaaccgt
     2581 ggcatccggc aggcgcgcct tgatcaggcg ctcgatatcg gcagcgtcca tgggcatcgc
     2641 cagctccttc ttgtccgctt ctggttggac ggacatccgc ggccctgccg tgagacgtga
     2701 ggccggctcg gtctcgttcc tgtgtccgcg acaagatacg cgaggcggct ctcgaccacc
     2761 agcggcacgc gccctcagtc ttgcggcgcg cctcagggtt tgccggttgg cgctgcgagc
     2821 cccgcctcga gacgctggcg ccgggcaagc agcatcgccg cggtgacgat cacggccatc
     2881 agcggcagcg cggcgatgtt gacgaccgtc cagccggcct tctgctggag gaagccggca
     2941 agcccggctg cgatggccgt cgtcgagtag acgcagaagt cgtgcgcagc ctgcacgcgg
     3001 gcgcgctccg ccggcctata ggttgtcgtg agcagcgccg agccgccgac gaaggtgaaa
     3061 ttccagccga gcccgaccag caggttggcg atgaggaagt tccagaagtc gatgcccatc
     3121 agattcacga tggcgcaacc cgtctggatg atggcgccag ccgcgatgat aggcagcgtg
     3181 ccgaagcgcg tgatcaggtg tccggtgaag aacgacggca gaaacatcgc gaccacgtgg
     3241 gcctggatga cggtggcact atcctcgaac ttgaagccgc aggcctgcat ggcgagtggg
     3301 gtcgagctca tgagcagcgt catgacaccg tagccgagca tcgaagtgat gagcgccgtc
     3361 aggaaggtct gcgagcgggc gatctcggac aaggggcgcc cgccctcggc gcgctcgctc
     3421 gccgtgaggc gcggaatgcg cacgaattgg acgacgatga gactcaagag gccgatcagg
     3481 gcaatcgtca cgtagacacc ggcgaagacc acgggcgcaa accagttaac gctccacttg
     3541 gcgagctgcg gcccgacgag gccggcgatg acgccgcccg ccatgacgag ggagatcgcc
     3601 ttcgccttgt agtcgggagg ggccgcatcg gccgcggcaa accggtagta ccaggcgaag
     3661 gctgcggccg agccctggat aagggtcgcg acgcagagga gcgtgaagct ctgcacgaac
     3721 actgcgtagg ccgcgagccc gccgaaaaga atgccgagga tcgccccgat cgagaagccg
     3781 gcgcgccgtc cgatgcgatc catcaacagc gaggcaggga ttgtcgtcag catgatgccg
     3841 gcgtgattga ggaagatcgg cagggtggca agcgacttgt cctcgccgag cagcgcatag
     3901 ccggcgagcg gcgacgtcgc gatgcccatc gactggacgg actggaagag accctgcgaa
     3961 acggcgagca gcccgacgtt tcgcttgttg ggcatactgc gctcaggagt gggcatgttt
     4021 cggcaaatcc ctgctcgatg cggtcgggtt tggcgagcat gtggcagctt agggcagtga
     4081 cgtatagctc cggcacgggg ctgggacatc acgcctcacg accgtggttg cgccgcgcac
     4141 atccttgggc cgcgattgcc gacgccctca ggtgcgtcga tagcgcagcg tggtggagta
     4201 ggccgcatcg agctgcagga tcgcctcgta gagcgcatgg ccggcgagcc gctgcttcag
     4261 ctcgatgacg aggtcctggc agaccggcag ctcagactcg gcaatgctcc tgagcacctg
     4321 gtactctttc gaggcgcgcg acaactcctt gtcgagtttg tcgcgggcct cgaggatctg
     4381 gcttgcgagg tcgaaggtga cgacaccgac ggccgcgcgg ctcgcgggcg caagatcgag
     4441 atgcggcggc cgggcgaggg gcacactcgc tgtcgggggc ggcggcggga cgcctgtcgg
     4501 ggccaacaga tccgcgggat gcgccggcag gtccatcgga tagatcggat cgagcgcgcg
     4561 cagcgcctcc ggcacgtcga agccgttcat aggaattgga ggcgggggga ttgccgctgg
     4621 gccggaaggc gggaccggcg tcggggacgg agcccagccg gtcgcttcga gcctcggcgg
     4681 ttcggacgcc gcgccttgcg aggggacagg gctgtcagcc cgcgctcgcc ggcctcgcag
     4741 aaaccctagg gccatggtgc gtgaccatga ttggcgccaa gcgatgggct catgcggcgt
     4801 cgagcctctg gagcgcggct tcgagatcgc gaaggcggat caaggtcacg aaatggtggt
     4861 tgcgctccag tgcattgcgc aactccgcgg tgcagcgtgc gcactcgctg aggccggcgt
     4921 cctcgatctg ctttaggaga cgatagggtt tagacgatgc ggatagtgcc gcctcgacgg
     4981 atgagatcaa cgagcgcacc tggcgcacga gattgtcgct cgaggtatcg agaacgagct
     5041 cggggacgaa gagtgccgcg cggcgctgac gccgctcctc gctggtcgcg cgcagtcgtg
     5101 gtgaggcgaa gggatcggag ctcgtgccgc tgctcgccgg tagcggcgcg cctcggctgt
     5161 ctgctggtgt gctcatgtgg cccccgctgc gacgcgacta tgtctgcagg ctcgcaacac
     5221 ctccgccagg agcggcgtgc ctgcgcggac gatagttgcg ggcatccatt tttccacaga
     5281 attatttggc tcggcgcgag tgtgtgttcc gacagaatgc ccgatatcgg aaaatggcat
     5341 ggtgcggctc gccctgggaa gcattcggaa cgccgtgatt gatccatgta ttacaatgtt
     5401 ttggcggaag tcttggaggt tgacggacaa atcgcggact gttgccgcta ttcggcggaa
     5461 agtggcgata tttccatgat gaagaaaggc cttttcccta gtgggagcaa ttggcacctc
     5521 gcaggtcgcc ttgccgctct tcgtgctcgg accgtcagtc tccgacgacg tggaggatga
     5581 cgcagcggtg atgcggcgcg ttgcgatgct cgaaaaggta gatgccctgc catgtgccga
     5641 gcgcgaggcg cccggccgag atcgggatcg agagcgacga ctgggtgagt gctgccttca
     5701 cgtgcgccgg catgtcgtcg ggtccttcgg cggtgtggat gaagagaggg tcgccgttcg
     5761 ggacaaggcg gcggaaaaat gcctggagat cgcgctgcac gtccggatcg gcattctcct
     5821 ggatgagcag cgagcatgag gtgtgctgac agaagacagt gcagaggccc gtcgaaagtt
     5881 ctgctccgcc gacaaactca atcgcctccc gagtgaactc gtagaggccc accccccgtg
     5941 tcgcgatctc tagcgtgcgc tgtctctgcc tgagtgtcac gagcggaaca gcctgtcgcc
     6001 attctgatcg ataatctggc gcgccttgtt gagggcgacg gttgccgcgt cttccatggt
     6061 ggcgaagcgg tcggccctga cgaagcgctc ttccttgcgc tcgccgccga tctccctggt
     6121 gatggtgccg gcaacctgga attgcccgcc ttcgggatag ggccgcgcct cgatgacgta
     6181 gcccttgtac tcctcgcggg ccgtgggacc cgccggttgc gtcggcgagc cgccgccgaa
     6241 tagcgatttc cagaatgaca tggagtgcct cgctcacagc ttgtggcggc atcttacacg
     6301 cggagccgca tctcaataaa gcagcgtcac agcaggtgcc cgagcactgg ggcacgcatt
     6361 ggtttgctca atcggttcgc agtgtcggca atgggatgct cgaatattcg ccacgcgacg
     6421 acattgcctt gacaccatgc atcttggcga gggaacctcg gttcggggag taggatgggc
     6481 gtgggcaggc gcgacttgca tcacgaccac ttgccggagt cgtgtgcgca tgttgcagcg
     6541 aagcggcgca gtatccaccc tggacttaag tcacggtcgc caagggagtg gcaacatgga
     6601 tcgcaggtcg ttcttgaaat cgggcctgag tgtcggcgct gtcgccgcct cagggaatct
     6661 ggcggcccca gccattgcgc agaaggcagc ggcaaagacg ctgcgcttcg tgccgcaggc
     6721 aaacctcgcg aacttcgatc cgatctgggg cacgcagtac gtcgtgcgca atgcctccca
     6781 gctcgtctgg gacacgctct acggcatcga cgacacgctg acggccaagc cgcagatgct
     6841 cgagggcgat accgtttcct ccgacggcct cacctggacc ttcaagctgc gcacgggcct
     6901 caagttccac gacggcgcgc cggtgctcgc caaggactgc gtcgccagca ttaagcgctg
     6961 gtcggcgcgc gacgccatgg gccagatgtt ggcgaagatc cagaaggagc tcgtcgccgt
     7021 cgacgaccgc accttcaaat gggtgctctc aaagcccttc acgaagatgc gctatgcgct
     7081 cggcaaggtc ggcacgccct gctgcttcat catgcccgag cgcttcgcct cgatcgatcc
     7141 gatgaagcag atcaccgagt tcatcggcgc cggcaagccg cacgccgagt tcgtcggctc
     7201 aggcccgatg aagttcaaga tcggcgactg ggtgccgggc gccaaggccg tgttcgagaa
     7261 gttcgacggc tatgtgccgc ggagcgagcc gtccaagtgg ttctctggcg gcaaggtgat
     7321 gctcgtcgat cgcgtggaat gggtgatcat gcccgatccg gcgacagcat cggccgcgct
     7381 ccagagcggc gaggtcgact ggtgggagag cccgatctcc gatctgatcc cggttctgaa
     7441 gcgcaaccgg aacatcaagc tcgatatcgc cgacccgctt ggcaacatcg gcgcgttccg
     7501 cttgaaccac ctgcatccgc ccttcaagga cgtgcgcgtg cgtcgcgccg tgcagatggc
     7561 gctgagccag gaagactaca tgcgtgcggt ggtcggcaac gatacggcgc tgtggagtac
     7621 gatcccgggc ttctttacgc cgggggcgcc cttctactcc gagcttggcg gcgagccgct
     7681 gaagggcccg cgcaacatcg acggcgccaa gaagctgctg gcggaggccg gctacagcgg
     7741 tctccccgtc acctgcctcg tcgcccagga tcagccgccg ctgaaggcga tgggcgaaat
     7801 taccgcggat ctcctgaaga agatcggcat ggcagtcgac ttcgtcgcga ccgactgggg
     7861 caccgtcggc cagcgccgcg cctcgaagaa cgagccgggg aagggcggct ggggcatgtt
     7921 ccacacctgg cacgccggcg ccgactgcgc gaacccggcg gcgcacatcg ccgtccgtgc
     7981 gggcggcgaa aaaggcccgg cctggttcgg ctggcccgag gacccggcgg ttgagaaggc
     8041 ggtcgacgag tggttcgagg cgaagtccgt cgaggacgag aaggccgcga ttgcgcgcgt
     8101 caacaaggcc gcgatggaga gcgtcgtctt catcccgacc ggccactaca agtcgtacca
     8161 ggcctggcgc agcaacatcg aaggcgtcgt caacggcccg ctcccctggt tctggggagt
     8221 gaaaaagagc tgagttcggc cggtagagtt cgacgagctt tacccgttac gccggaggcg
     8281 tgctttaagc acgacgagca ccccgccagg gacttgtccc tggctcccgg tgtacatgag
     8341 gtttccaaag gcccaaggcc tttggcgggg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnngctgagt gtgcagtctt tggagaccaa cgccgcacga tgctcaccta catcttgaga
     8461 cgcgtcctgg cgacgatccc ggtcatggcg atcgtcgcct ttctcgtgtt ctcgctgctc
     8521 tacatcgcgc cgggtgaccc cgcggccgtg ctcgccggcg agcaggcctc gcccgaggac
     8581 gtcgagaaga tgcggaaatc gctcggcctc gaccggccgt tcctcgttcg ctttgtcgaa
     8641 tggtcggcgc agatcctgca gggtaacttc ggcacctcgc tgttcacggg gctccccgtc
     8701 actacgatga tcggccagcg catcgggccg accctgtcgc tcatgatcgt gacgctggtg
     8761 ctcgcggtgc tgatcgccgt gccgatgggc gtgatcgcgg cctggaaggc agggacgctc
     8821 ctcgaccgtg tgctcatggc cttcgccgtc gtcggcttct cggtgccggt gttcgtcgtc
     8881 ggttatctcc tcgctttcta tctcggcgtc gaatgggact ggcttcccgt gcagggctac
     8941 cgggagctct ccgaaggttt ctggccctgg ctccagcatc tcaccttgcc ggcgatcgcg
     9001 ctcggcttcg tctacatggc gctgatcgcg cggatcacgc gggcgacaat gctcgaggtg
     9061 ctctcgcagg actatgtgcg cacggcgcgc gcgaagggcg tcgggcaggc gagcatcctc
     9121 tttcttcatg cgctcaagaa cgccgcagtg ccgatcgtca ccgtgatcgg catcggcatt
     9181 gccttcctga tcggcggcgc ggtcgtcacg gagagcgtgt tcgcgatccc agggctcggc
     9241 cggttgacga tcgattcgat cagtcgccgc gattacccga cgatccaggg tatcgtgctg
     9301 ctgttcagct tcatctatgt gctcgtcaat ctggcgatag acatcatcta caccctgctc
     9361 gacccgagga tacgctattg agctcggatg cctcaccgat tgcggcgccg gcggcggctc
     9421 cggcgctccc cgacatcttt cccccggtca ggccgcggac gggggtgtgg ggtgtcgtct
     9481 accgccatcc gaccatggtc atcggcggag cgatgctgtc gctgctcgcc atcttcgcgc
     9541 tgttcgcgcc gtttctcggc accgtcgata tagatgcgcg cgaactcaag ctgcgcaatg
     9601 ccgatccctc agcagccaac tggttcgggc gcgacgcgct cggccgcgac atctacacgc
     9661 gcgtcgtcta cgggacgcgc atctcgctca ttgtcggcgt gtcggtcgcc atcctcgcct
     9721 cgctcgtcgg gctttcgatc ggcctcgtct cgggcttcgt gcgctggtcg gacggcatcg
     9781 tcatgcggat catggacggg ctcatgtcga tcccgccgat cctgctcgcc atcgcgctga
     9841 tggcgttgac cggcgcgagc gtgaagaacg tcatcatcgc catcacgatc gccgagatcc
     9901 cgcgcgtctc gcggctcgtg cgcggtgtcg tcctctcgct gcgcgagcaa ccctacgtgg
     9961 acgccgcgat cgccgcgggc acctcgacgc cgatggtgat cctgcgccac atcctgccga
    10021 atacgctggc gcccatcacg gtgcaggcga cctacatctg tgcctcggcg atgatcacgg
    10081 aggcgatcct ctcgttcgtc ggcgctggca ttccggcggc catggcgccg tcctggggga
    10141 acatcatggc ggacggtcgc tcggcctggc aggcctatcc gcacgtcatc ttcttcccgg
    10201 cgatcttcct gtcgctgacg gtgctcgccg tgaatctgct cggcgacggg ctccgcgacg
    10261 cactcgaccc gcgcatggcg aagaggatct gagccatggc gctgctcgag gtcgagaacc
    10321 tgcagaccca cttccgcacg atcgacggtg tcaaccgggc agtcgacggc gtctccttca
    10381 ctctcgaggc cggcgagacg ctggcgatcg tcggcgaatc gggctgcggc aaatccgtga
    10441 cggcgatgtc gctgatgcgc ctcatcgcca tgccgccggg caggatcgcc ggccgcgtct
    10501 cgttcgcggg ccgtgatctc ctcgccgcga gcgagcgcga gatgcgtcag atccgcggca
    10561 acgagatcag catgatcttc caggagccga tgacgagcct caatcccgtg ctgaccattg
    10621 gccgccagct cggcgagacg ctgcgtctcc atcagggtct cggcaagacc gaggcgacgg
    10681 cgcgcgcggt cgagatgttg aggctcgtcg gcatcccgga ggccgagcgc cgcgtcaccg
    10741 agtatccgca ccagatgtcg ggcggcatgc gccagcgcgt catgatcgcc atcgcgctcg
    10801 cctgcaatcc gaagctgctc atcgccgacg agccgacgac cgcgctcgac gtcacgatcc
    10861 aggcgcagat cctcgacctg atgcgcgacc tcaagcggag aatcggcgcg gcgatcattc
    10921 tcatcacgca cgatctcggc gtcgtcgccg aggtcgccga gcgcgtcatg gtgatgtatg
    10981 ccgggaggaa ggtcgaggag gcgccggtcc gcgcgctttt ccacgcgcct cgccatccct
    11041 atacgcgcgg gctcctcggg tccgtgccga agctcggctc gtcggtcacg gggtcggagg
    11101 agcggctggc cgagatcccg gggctggtgc cgagtctcaa gagccgcatc gagggttgcg
    11161 tgttcgcctc gcgctgtccg atcgcgacgg aggtctgccg ggaggtcgcg cccgcgctcg
    11221 aagcgaaggc ggcccgtcac gtcgtcgcct gccatcacgc cgcgcgggag gcggtcaccg
    11281 catgagtgcg cccctcctcg aggtccggaa cctcaagaag catttcccgc tgacggcgag
    11341 ccttctcggc cgctcgcgcg ggttcgtcta tgcggtcgac ggcgtctcct tcgaggtcgc
    11401 gaagggtgag acgctggcgc tggttggtga atccggttgc ggcaagtcga cggtcggtcg
    11461 tgcgatcctg cgacttttcg atatcacctc gggcgaggtg ctgatcgacg gccagcgcat
    11521 cgatgagctc tcgagcggca acctccgtcc gatccggcgg cgcgtgcagg tcgttttcca
    11581 ggacccgttc tcgagcctca atccgcgcat gcgcgtccgc gacatcctgg ccgagccgat
    11641 ccgaaacttc ggcctcgcca aggacgaggc cgatctcgac cgccgtgtcg gcgacctgat
    11701 ggacaaggtg cggctcccgc gcgaggccgt cgggcggtgg ccgcacgagt tctcgggcgg
    11761 gcagcgccag cggatcggca ttgcccgcgc gcttgcggcc gagcccgatc tcatcatctg
    11821 cgatgaagcc gtgtcggccc tcgatgtctc ggtgaaggcg cagatcgtca acctcttgca
    11881 ggatctgcag cgcgagctcg gcctcgcact cctcttcatc tcgcacgacc tcgccatcgt
    11941 cgagcacatc acgcatcgtg tcgcggtgat gtatctcggc aagatcgtcg aggtagcgcc
    12001 gaagcggtcg atcttcgctg ggccgaagca tccctatacc gaagcgctgc tctcagccgt
    12061 gccggtgccg gacccggacg cgccccgcaa ccgcattctc ctgaagggcg atgtgccgag
    12121 cccgatcaac ccgccgaaag gctgccgctt ccacacgcgc tgcccaaagg cgttcgaccg
    12181 ctgccgggtc gaggaaccgg cgttgaagcc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc gggtccgggc
    12361 ccgcggcccg gtcgcgcagc gaccctctca cgtcgcgcac gccgggcatc gcgcccctgc
    12421 ctgcccctga ccttcggcgg actcgaaggc cgcgccaagg tgtactagag agagtcgaga
    12481 ttcgtgtgcc ggcgctaccg gcgtcccaat gggccggtgg cgtcgtggcc attgtgcgcg
    12541 ccaaccctcg gagcccccta tgacccgcca gcccgagaat gacgagcttg cccggacgtc
    12601 cttcctgaat ggcgtcaacg ccgcctacat cgaggagctg caggcgcgtt acgatcagaa
    12661 cccgggctcg gtgccggatg aatggcggct attcttccag agcctgaagg agggacctgg
    12721 ggccgcatct gccgagcgcg aggggccctc ttgggggcgg ccgatttccg agatgcagca
    12781 gaacggcgag ctggttgcgg cgctgactgg tgactatgcc cccgtcgaga gggagctgcg
    12841 cgatcgcatt tcgcaccgcg cgcaatcggc cggcgtcgag gtgtcgccgg ccgcgacctt
    12901 ccgcgcaaca caggacagcc tgcgcgcgct gatgctgatc cgcgcttatc gcgtaatggg
    12961 ccacctcgtc gccgatctgg accccctcgg catcagtgag cgcaaagtgc acaaggagct
    13021 tcggcccgag acctacggct tcacggaagc ggatctcgac cggccgatct tcatcgacaa
    13081 ggtgctcggc ctcgagacgg cgacggtgcg ccagatcctg aaaatcctgc gccgaaccta
    13141 ctgccgccac atcggcgtcg agttcatgca catcacgagc cctgcgcaga agcgctggat
    13201 ccaggagcgc atcgagggcg ccgacaagga cgtgacgttc acgcgggagg gcaagcgcgc
    13261 catcctgaac aagctgatcg cggcggaagg cttcgagaag ttctcggaca tcaagtacac
    13321 ggggacgaag cgcttcgggc ttgatggcgg tgaatccatg atcccagcgc tcgagcagat
    13381 tatcaagcgc ggcggtcagc tcggcgtgaa agagatcgtc attggcatgg cgcatcgcgg
    13441 gcgcctcaac gtgcttgcca acgtgatgtc gaagccgctg cgcgcgatct tcaacgagtt
    13501 caagggcggc tcctacaagc ctgaggaggt cgaaggctcg ggcgacgtta agtaccacct
    13561 cggtgcctcg tccgaccgcg agttcgacgg caacaaggtg cacctctcgc tcactgcgaa
    13621 cccgagccat ctcgagatcg tcgacccggt cgtgctcggc aaggtgcgcg cgaagcagga
    13681 ccagcaccgc tgcgagccgg gcgagcgcac gcccgtactg ccactgctca tccatggtga
    13741 cgcggccttc gccggccaag gcgtggttgc ggagtgcctc ggcctctcgg gtctcaaggg
    13801 ccaccgcacc ggcggctcga tccacttcat catcaacaac cagatcggct ttacgacgaa
    13861 cccgcgctac tcgcgctcct cgccctattg caccgacgtc gccaagatgg tggaggcgcc
    13921 gatcttccac gtgaacggcg ataacccgga ggcggtggtg cacgtcgcca aggtcgcgac
    13981 cgagttccgc cagaagttcc agaagcccgt cgtcatcgac atggtctgct atcgccgctt
    14041 tggtcacaac gagagcgacg agccggcctt cactcagccg atcatgtacc ggaagatcaa
    14101 ggcccatcag accgttgtct ccatgtacag ccgccgcctc gtcgaggaag gcgtgatgag
    14161 cgaggccgac gtcgagggca tgcagacgag cctgcgcgcg cacttcgacg gtgagctcgc
    14221 tgcggccgag ggctacaagc cgaacaaggc cgactggctc gacgggcgct ggtcgcagat
    14281 cgggctcccg gaggacgagg cacgccgcgg caacacaggc ctttcgatcg agacgctgaa
    14341 ggatgtcggc aggcggatta ccacggttcc gcacgatttc cagacccaca agacgattgg
    14401 ccgcctgctc gagcgccggc gcgagatgat cgaaacgggc gaggggatcg actgggcgtt
    14461 cgccgaggcg ctcgccttcg gcacgctcgt caaggagcgc tttgcggtcc gcctctccgg
    14521 ccaggacgtc gagcgcggca ccttttcgca gcgccacgcg gttctgatcg accaggagag
    14581 cgagcggcgc tacacgccat tgaagtacgt ggcgcccgat caggcccgct tcgaggtgat
    14641 caactcgatg ctctcggagg aggccgtcct cggtttcgag tacggctact ccctcgccga
    14701 gccgatggcg ctcgtcatgt gggaggcgca gttcggcgac ttcgccaacg gcgcgcaggt
    14761 ggtgttcgac cagttcctct cctcgggcga gcggaagtgg ctccgcatgt ccggcctcgt
    14821 ttgcctgctg ccgcacggct acgaagggca gggcccggag cattcctcgg cgcgtctcga
    14881 gcgcttcctc cagctttgcg cggaggataa ctggcaggtc gccaactgca cgacgccgtc
    14941 taactacttc cacatcctcc gccgccagct gcagcgcaag gtcaggaagc cactcatcct
    15001 gatgacgccg aagtcgctgc ttcgccacaa gcgcgtggtc tcgcggctcg acgagatggg
    15061 gccggggacg agcttccacc gcgtgctctg ggatgatgcg cagcatctcc ctgggcagaa
    15121 gatcaagctg aagccggacg gcgaaatccg ccgcgtcgtg ctgtgctcgg gcaaggtcta
    15181 ctacgacctc tacgaggcgc gcgaggccgc cggcatcgac gacgtctata tcctgcgcgt
    15241 cgagcagctc tacccgactc ccgtccgcgc cctcatccaa gaactgcagc gctttccgaa
    15301 gtccgacgtg gtctggtgcc aggaagagcc gcgcaacatg ggcgcctgga gcttcatcga
    15361 gggtcagctc gagtgggtgc ttgcccacac gaacggcgcc gtgaagcggg cgcgctatgc
    15421 cggccgcgcg gcctcggcct cgactgcgac gggcctcgcc tccaagcatg cgagtgaaca
    15481 gaaggcgctc gttgccgacg ccctggggtc ggcggcagca tgagcgatcg cacgggcaag
    15541 agcgacgtcg cccgctatta cgacttcatc tacctgtgga cgcagctgac caaccggttc
    15601 ggcgcgttcc gggtgccgca ggcgcacacc atccaccgta tgctggtgga ccccgagacc
    15661 gggagcttcg acggcgagac gatccatcgt ctcatcgcca ggcacgccaa gagcctggca
    15721 cctgtcaggg cgctcgatgc cggctgcggc tacggcggca cctgcatggc gatgtcgaag
    15781 ctcgacgagg gacactggca gggcatcaca atcaaccgaa cgcaggttcg cgtcgccgca
    15841 cgcaatgcca aggccctcgg cctcgatggc aaggtcgcga tcggccacgg ttcctatgac
    15901 gcgccgctca gccagaccta caatctgatc tatggcatcg agagtctcgt ccacagcgtc
    15961 gacccgtccc aaaccatcgc gaaccttgcc aaggcgctcg atccgggtgg tctcttcatc
    16021 atcattgacg acatgccggt cgaggacgtg ccggcggccg cgcgcgccga tctcgagatg
    16081 ttcaaggcca gctggcgctg cccggtcatg ccgacggccg cgggctggca gaagcgtctg
    16141 acgggagctg gcctcgagat cgcggcggtt gatgatctct cgccgctctc gcgtcccgga
    16201 acccacgagc acatcgagcg gagcctagcc gaagtcgcgc gccgccgccg ctggatggcg
    16261 ccactcggca tgagcatggt gacggacgcg caggtcggcg gcctcgtgct cgaacgcctc
    16321 tcgcgcgacg gagctgtccg ctacatgatg atcgtagcgc gccggcccag gtaaaccgat
    16381 cccaacttcc atcgagacta cagaaacgga acgaacgaca tgaccatcga gatcaaggtg
    16441 ccggcgctgg gagaaagcgt caccgaggcg acgctctcca agtggtacaa gaacgccggc
    16501 gatccggtga aggccgatga accgatctgc gaactcgaga cggacaaggt gacggtcgag
    16561 gtgccggcgc cggccacagg cgtcatgggc gagcacaaga tcggcgcggg ctcgaccgtc
    16621 gccgtcggca cggtgatcgg aactctgaag cccggcgctg cgggcgccag tgtcgcagca
    16681 gccgctccag ctgcagtgtc acctccgaaa cctgcggcaa cgcctgccgc tcctccgccg
    16741 gccgctccga gtgccatgcc cccgtcgcct gcggcccgca agatcctcga ggagaagggt
    16801 gtcgccagcg ccgacgtcgc cggaaccggt cggcgcggac aggtgatgaa gtcggacgcg
    16861 atggctgcca ctgcggcgcc cgccccgcag ccggccgccg cgtcgctgcc ggtgacgcg
//
LOCUS       DAORNH010000067        16716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2621_length_16716_cov_2.572235, whole genome shotgun sequence.
ACCESSION   DAORNH010000067 DAORNH010000000
VERSION     DAORNH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16716
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_16716_cov_2.572235"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK264_09030"
     CDS             complement(<1..608)
                     /locus_tag="PK264_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD76071.1"
                     /translation="MFKRRPPAGPGSAPTAAELDRLMALAARAAECRDYDAALGHWST
                     CAHHGVARAQAEIGRCFVDALGVERDTKLAHTWLKLAADAKDALGQRLLGDYYFNGEG
                     GSPDRAIAEEWYERAAAQGEPLAQEMLSWIYAEGDLRTPDYAKARHWAERAAAQGIAK
                     SKTRLGFLYHNAMGVARDAGVAAKWWREAARGGDADGQAMYGA"
     gene            complement(611..>945)
                     /locus_tag="PK264_09035"
     CDS             complement(611..>945)
                     /locus_tag="PK264_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76072.1"
                     /translation="FQAALGLRPRVPGLRAALPAAIEIDVVDCASQIGSGALPLETLE
                     SAGLRLASRSIKAESIAAALRKAPIPVVGRINDGAVILDLRCLDDPRAFAAGLTGAAS
                     ALSARTEA"
     assembly_gap    946..955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<956..2069)
                     /gene="selA"
                     /locus_tag="PK264_09040"
     CDS             complement(<956..2069)
                     /gene="selA"
                     /locus_tag="PK264_09040"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRD76073.1"
                     /translation="MDDARPLARPRLPAVDEVLRSTAGRALAARYGHAATVAAIREVI
                     ALHRAAELREGTAIAPEAVAVKSGTILEARDQPTLRRVFNLTGTVLHTNLGRAVLSES
                     AIEAAVAAMRHAANIEYDIPTGARGERDDHVRGLVREVTGAEDCIVVNNNAAAVLLTL
                     NELGNGREAIVSRGELIEIGGAFRLPDIMARAGARLVEVGTTNRTHLKDFEGAIRPET
                     GLIMKAHTSNFLIQGFTAAVEPAALARLAKSKGVPFIDDLGSGTLVDLRRYGLKHEPT
                     VQQAIEAGADLVTFSGDKLLGGPQVGIVAGRKDLVARLARNPLKRALRIDKVRMAALA
                     ATLKLYRDPDRLTETLPTLRAFVRTRAELRGVGEGLL"
     gene            complement(2127..3026)
                     /gene="fdhE"
                     /locus_tag="PK264_09045"
     CDS             complement(2127..3026)
                     /gene="fdhE"
                     /locus_tag="PK264_09045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HRD76074.1"
                     /translation="MSEGGQVKSGLMSIGEEAKPPFAVAPAPVAVFAARAARLEALAA
                     GHAVEGYLRLVAGICQAQAEVVTGLPPVSAPLASHQGMPPLQVDTLQVEGSLEETLVA
                     LSHALANLDAPADFKVAIGRIRDREPEARRALLAAIVDGEGAGDDIATEVIATAALQV
                     HLTRLAALVDAEKLEPVADGVCPACGSPPVASAVVSWPNASNTRFCTCSLCATQWHVV
                     RLKCASCGSTAGVSYPHIEGQKETLRAETCDVCRRYVKIVYQIKDPAHEPFADDIATL
                     DLDLVLKAEGWTRGGRNPFLLGY"
     gene            complement(3102..3797)
                     /locus_tag="PK264_09050"
     CDS             complement(3102..3797)
                     /locus_tag="PK264_09050"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HRD76075.1"
                     /translation="MSGSGRGDVHVRRYSGSARLNHWIMAISFVLLLLSGLSLFHPSL
                     FFLSGIFGGGATVRWLHPWVGLVFAVTFLLLFVRFLPANLPERTDIAWLARLKHVLAG
                     RDEYLPEVGKYNAGQKLVFWLQFILVPILLVTGFGLWEPGLKWAEATFSFKATMDQKR
                     MAALLHAIAAVAAITIWIVHVYAAIWVRGTISAMTRGTVSGGWGWRHHRKWLKTEVSE
                     GRVEKKSGTAPAE"
     gene            complement(3794..4696)
                     /gene="fdxH"
                     /locus_tag="PK264_09055"
     CDS             complement(3794..4696)
                     /gene="fdxH"
                     /locus_tag="PK264_09055"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HRD76076.1"
                     /translation="MATFQPLDVVRKSATVTAAPPAKRGVEVAKLIDVSKCIGCKACQ
                     SACLEWNNLKEPVGTNAGVYDNPHDLTPATWTLMRFTEYENPKSGNLEWLIRKDGCMH
                     CEDPGCLKACPAPGAIVQYANGIVDFQKEHCIGCGYCEKGCPFNIPRISQVDNKAYKC
                     TLCSDRIAIGQQPACAKVCPTHAISFGTKAEMVAHAEKSITVLKARGYDKAGLYNPPG
                     VNGTHVMYVLHHADQPQLYANLAQTPRISPLVEAWKGVSKYAGMAVIGATALVGALHY
                     FLKGPNEVTDHDEAEAKRLAETKK"
     gene            complement(4711..>5532)
                     /locus_tag="PK264_09060"
     CDS             complement(4711..>5532)
                     /locus_tag="PK264_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HRD76077.1"
                     /translation="NMARRDTRDPDGTGSYQTWAFSWPANRRILYNRASADMKGQPWD
                     PKRRLISWDGTRWSGYDVPDIAPTAKPDVVMPFIMTEEGVGRLWVRGLMKDGPFPVHY
                     EPFESPIANPIAPKVRGNPAARVFKGDMEVFGTAKDFPYAAVTYRLTEHFHFWTKHVS
                     VNATLQPELFVEISEELAKEKKIIKGGWVRVWSNRGSVKAKAVVTKRIKPITCDGKVV
                     HIVGIPIHWGFTGAAKKGFGANILTPFVGDANIETPEYKAFLVDIEPIATGPAVS"
     assembly_gap    5533..5589
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5590..7780)
                     /gene="fdnG"
                     /locus_tag="PK264_09065"
                     /pseudo
     CDS             complement(5590..7780)
                     /gene="fdnG"
                     /locus_tag="PK264_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912978.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
     gene            complement(7892..10186)
                     /gene="selD"
                     /locus_tag="PK264_09070"
     CDS             complement(7892..10186)
                     /gene="selD"
                     /locus_tag="PK264_09070"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRD76078.1"
                     /translation="MRVTPILKDLVLIGGGHAHVHVLKSFGMKPIPGVRVTLLSRDIE
                     TPYSGMLPGFIAGHYTKAECHIDLRPLARFAGARLIAGGAIGIDRSARRIRVQDRPDI
                     AFDVLSIDIGSTPTYDGIAGARQHATPVKPIDGLARHWRRVLEIAEAAPRPLQFLTVG
                     GGAAGVELTLAAQHRLKSLPKAQVRDHAFTLLTRGELLASHNARVRTAFRRELRNRNV
                     ALHEHCAVAEVGRNGLTTEDGRAFAFDELLWVTDAGAAPWLRETGLAIDGHGFISVDA
                     CLQSTNTPGVFAAGDIASSTVHPRPKAGVFAVRQGPPLQENLRRALAGSELQPFKPQT
                     NFLSLISTGDKVAVASRGALALSGPRLWTLKDWIDRRWMDGYQSLRPGMGGETAKAAG
                     DDIGAAALASLRCGGCGAKVGANALSRALARLAPKASAGAVIALGEADDAAVIAPPPG
                     MHLVQTVDFFRAFIDDPYLFGRIAAVHALGDVYAMGGAPLTALATAVIPYAAEAMVED
                     DLYQMLRGALDVLEPAGCALVGGHSAEGAELALGFAINGAVDPSRILRKGGVRPGDAL
                     ILTKPLGTGALFAAEMRGAAEAGWIAAALKVMQQSNAKAAKIAIDAGATACTDVTGFG
                     LAGHLLEMLGASRCDATIDLGKLPVLAGALDVIGRGIESSIAPENVRVERAIESRPGV
                     GSGPRYRILFDPQTAGGLLFAMPASEAEGCIRSLMAASYCDAAIIGIAREPSGLEPKV
                     LLQEIENPKTSFSQSKDGAVLVAG"
     gene            10459..10836
                     /locus_tag="PK264_09075"
     CDS             10459..10836
                     /locus_tag="PK264_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRD76079.1"
                     /translation="MRFSLRLGAGIAALTGFATAAMAVEPTGNWVRPSSGTVINFYAC
                     GANLCAKIVSVKDASKKGTVGTVIMNGAVKSGANVWKGSLLNTEDGQTYTGSVTLSGS
                     GLVLSGCVLGGLICKNETLQRVK"
     gene            10833..11711
                     /locus_tag="PK264_09080"
     CDS             10833..11711
                     /locus_tag="PK264_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HRD76080.1"
                     /translation="MSTAAARLPGHRLHLQHGPIDLVIGAFGPGGEVERAYAAAEARF
                     PRVLPELVEELPVLRAPIGDARPVVAGEIAQRMLTAVWPYRDRFITPMAAVAGAVAEV
                     MLEVMGNAAALDRAYVNNGGDIAIHLASGHAMAIGVVGDLADPAIDGRVTITADDRVR
                     GIATSGWRGRSLSLGIADAVTVLATTASRADAAATMIANAVTVDHPAIRRAPARSVRS
                     ESDLGDMPVTVDVGELPAALRRRALGAGQRRAEELIGAGLIEAAYLRLQGQSIVVDHG
                     RDTARRLVDGQARGAM"
     gene            12062..13240
                     /locus_tag="PK264_09085"
     CDS             12062..13240
                     /locus_tag="PK264_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD76081.1"
                     /translation="MGTYGRQDIVFERGEGSWLVSTTGERYLDFGSGIAVNLLGHAHP
                     HLVKGLAAQAEKLWHTSNLYRVAGQEMLAERLVAATFADKVFFCNSGAEACEGAIKLA
                     RRYQYTIGKPERWHIITFKGAFHGRTLATIAAAGNEKYLEGFGQPTPGFKVLPEVNDL
                     AAVEAAVTPEVGAIMVEPIQGEGGIRSCTTEFLKGLRAICDKHGLLLVLDEVQCGMGR
                     TGKLFAHEWAGITPDVMAIAKGIGGGFPVGAFLATEQAAKGMVPGTHGSTFGGNPLAM
                     AVGNAVLDVALEKGFLDGVQTRALRFKQGLARLKDENPDAIEEIRGQGLMMGLKLKPA
                     VAVADVVKACMAEKLLMVSAGENVLRLLPPLNATEDELAEATKRIGAALKSVAKPRTA
                     "
     gene            13237..14214
                     /gene="argF"
                     /locus_tag="PK264_09090"
     CDS             13237..14214
                     /gene="argF"
                     /locus_tag="PK264_09090"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRD76082.1"
                     /translation="MSERRTTTSITTKASGAKAPRHFLDLDRIDAKALRRIVDMGHAM
                     KAAGKRTPKALRPAGIEDQVLVMIFEKPSTRTRVSFDVAMRQLGGQTIALNHADLQTN
                     RGEPVEDTARVLSRYADAIMIRANKHETLLGLAENASIPVINGLTDLSHPCQVIADIM
                     TFEEGGRGAIAGRTVAWLGDGNNVAASWMHAAVKFKFKLRLACPPQLNPEKQVLDWVR
                     REGGDVELSDDPQAAVKAADCVVTDTWVSMGQTDAPRRKQLLGPYAVDDALMTKAAKG
                     AIFMHCLPAYRGHEVSSSVIDGPQSVVFDEAENRLHAQKAILAWCLGVD"
     gene            14214..15218
                     /locus_tag="PK264_09095"
     CDS             14214..15218
                     /locus_tag="PK264_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HRD76083.1"
                     /translation="MMGGASAARGGTLPSVPDDLALPFRTVASDVAGRLARLGPAIDT
                     VLTRHDYPEPVAIALGEALLLAALLGNPLKDGGRLILQTKSDGPLGLLVANYDAPGRL
                     RGYASFDREKLATLAAGGPVSQASLLGSGHLALTLDPGGEQTSYQGIVALEAGRPGEA
                     GSSALADAAHAYFRQSEQLPTFIRLAVGRQFLKGGDGREPGWRWRAGGIIVQHVPKEG
                     GDERRGKLLDDDRLDGEDDDNWRRVRILSETVSAEELIDPLLAPESLLFRLYQEEGVR
                     VTPATALEARCRCSRERIATFLRRFQGEERTSLAEADGLIKVTCEYCSSKYQFKPAEL
                     "
     gene            complement(15225..16109)
                     /locus_tag="PK264_09100"
     CDS             complement(15225..16109)
                     /locus_tag="PK264_09100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HRD76084.1"
                     /translation="MNYLETLVRLDGAALAEVARARDGREIPPGLGAEARRAVDLIRA
                     ELEAVAVLVAVSASVVAAIASGRQGRSLAKSIDTYLPLELALVTHVAAPLLASEPGIE
                     LLADVRAFEIRLDLLRRMSRAFAADAREGTPGRSGVAIDILADGWRSLCETAIAVLEA
                     TAGLTEVDREHASHLSSLLARAEAGHADCIDADGAISIPGWAERRRHQRFPLAETATL
                     VIGDAIHPVTISDASIGGLGICDAPGLVRVQEVAVRLKDGRRLSGRIAWTRGNQSGLK
                     LEMPLDPADPLLSSGIAV"
     gene            complement(16180..>16716)
                     /locus_tag="PK264_09105"
     CDS             complement(16180..>16716)
                     /locus_tag="PK264_09105"
                     /inference="COORDINATES: protein motif:HMM:NF018534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HRD76085.1"
                     /translation="HLLTDAQDALPAVGFDGRYTIAVWVGRPDATATPGLTGRTAAAP
                     LLFDAFAALGPRRVPLATAPGGAIIASGAQLPMPLRRFKEAGDEARAGAYLDPGVQIA
                     FPPDRSVLEMEPGEDLRLALKAEGGALPLTWLIDGEPIAEPAQSHEIEWLAAGRGFAR
                     ITVIDARGRTDRVTVRIK"
BASE COUNT         2760 a         5534 c         5518 g         2837 t
ORIGIN      
        1 gcgccgtaca tggcctgacc gtcggcatca ccgccgcggg ccgcctcgcg ccaccatttg
       61 gccgcgacgc cggcgtctcg ggcgacgccc atcgcgttgt gatagaggaa gccgagccgg
      121 gtcttggatt tcgcgatccc ctgtgccgcg gcacgctccg cccaatgcct tgccttggcg
      181 tagtcgggcg tgcgcaggtc gccctcggcg tagatccagg agagcatctc ctgtgcgagt
      241 ggctcgccct gcgccgccgc ccgctcgtac cactcctcag cgatggcgcg gtctggcgag
      301 ccgccttcgc cgttgaagta gtagtcgcca agcaggcgct ggccgagggc gtctttcgcg
      361 tcggccgcga gcttcagcca agtgtgcgcg agcttcgtgt ctcgctcgac gccgagggca
      421 tcgacgaagc accgcccgat ctcggcctgg gcgcgcgcga cgccgtggtg ggcgcaggta
      481 ctccagtgtc caagtgctgc gtcgtagtct cggcattcgg cggcacgcgc ggcgagcgcc
      541 atcaggcgat cgagctcggc ggcagtcgga gccgagcccg ggccagcagg cgggcgacgc
      601 ttgaacatgc tcacgcctcc gtccgtgccg acagggccga ggccgcaccg gttaaaccgg
      661 ctgcgaatgc gcgcgggtcg tcgagacagc ggaggtccag tatcacggcg ccgtcgttga
      721 tgcggccgac gaccggaatc ggcgccttgc gcaacgcagc cgcgatcgac tcggctttga
      781 tcgacctgct tgcgaggcga agaccggcgc tttcgagcgt ctcgagcggc agcgcgcccg
      841 agccgatctg gctcgcgcag tcgacgacat cgatctcgat cgcggcgggc agcgccgcgc
      901 gcaagcccgg cacccgcggc cgcaagccca aggcggcctg aaaccnnnnn nnnnngcagc
      961 aggccctcgc ccaccccgcg gagctccgcc cgcgtgcgca cgaaggcgcg cagcgtcggc
     1021 agcgtctcgg tgaggcgatc gggatcacga tagagcttca gcgtggcggc gagtgcagcc
     1081 atgcggacct tgtcgatgcg cagcgcccgt ttcagcggat tcctggcgag ccgcgcgacg
     1141 agatccttgc gtccggcgac gatcccgacc tgcggaccgc cgagcagctt gtcgcctgag
     1201 aacgtgacga ggtcggcgcc ggcctcgatg gcctgctgca ccgtcggctc gtgcttcaag
     1261 ccgtagcggc gaagatcgac gagcgttccc gatccgagat cgtcgatgaa ggggacgccc
     1321 ttgcttttcg cgagccgggc aagggccgcc ggctcgacgg ccgcggtgaa gccctggatc
     1381 aggaaattcg aggtgtgagc cttcatgatg aggccggttt ctggccggat cgcgccctcg
     1441 aaatccttca agtgcgtgcg gttggtcgtg ccgacctcga cgaggcgcgc gccggcacgg
     1501 gccatgatgt ccggcaggcg gaaggcgccg ccaatctcga tcaactcgcc acgcgagacg
     1561 atcgcctcgc ggccattgcc gagctcgttc aatgtcagca gcacggcggc ggcgttgttg
     1621 ttgacgacga tgcagtcctc ggcgcctgtg acctcgcgca cgaggccgcg aacgtgatcg
     1681 tcccgctcgc cgcgcgcccc ggtcgggatg tcgtactcga tgttggccgc atgccgcatg
     1741 gcggcgaccg ccgcctcgat cgctgattcc gagagcacgg cacggccgag gttggtgtgc
     1801 agcacagtgc cggtgagatt gaacacgcgg cgcagcgtcg gctggtcgcg ggcctcgagt
     1861 attgtgcccg atttgaccgc gacagcttcc ggcgcaatgg ccgtcccctc tcggagctcg
     1921 gcggcgcgat gcagtgcgat cacttctcgg atagcagcga cagtcgcggc atgcccgtag
     1981 cgtgcggcga gtgcgcgccc ggcggtcgac cgcagcacct cgtcgacggc gggcagcctc
     2041 gggcgggcca aaggcctggc gtcatccatg acagcgcgag cctcgtgttg ctccggcgga
     2101 accgttcgtt ccttggccgg cgcccatcaa taaccgagca ggaacgggtt gcgcccgccc
     2161 cgcgtccagc cttcagcctt cagtactagg tcgagatcca gcgtcgcgat atcgtcagcg
     2221 aagggctcgt gcgccggatc cttgatctga tagacgatct tcacatagcg cctgcagaca
     2281 tcgcaagtct cggctcgcaa cgtctctttc tggccttcga tgtgcggata ggagacaccc
     2341 gccgtcgagc cgcacgaggc gcacttgagc cgcacgacat gccactgcgt cgcgcacagc
     2401 gagcaagtgc agaagcgcgt gttgctggcg ttcggccagg acacgacagc cgatgcgacg
     2461 ggcgggctgc cgcatgcagg gcagacgccg tccgcgacgg gctccagctt ctcggcgtcg
     2521 acgagtgcgg caagccgggt caggtggacc tggagtgcag cggtcgcgat cacctcggtg
     2581 gcaatgtcgt cgccggcgcc ctcgccatcg acgatcgcag ccagcagcgc acgccgcgcc
     2641 tccggctccc ggtccctgat cctgccgatt gccaccttga agtctgcagg agcatcgagg
     2701 ttggcgagcg catgggagag cgcgacgagc gtctcctcga gcgaaccttc gacctggagc
     2761 gtgtcgacct ggagaggcgg cataccttga tgcgaggcta gcggcgcgga tacgggcggc
     2821 agacccgtca cgacctcggc ctgtgcctgg cagatgccgg caacgagccg caggtagcct
     2881 tcgactgcgt gccccgccgc gagagcctcg aggcgcgcag cgcgagcggc gaacacggca
     2941 accggcgccg gcgcaaccgc aaacggcggc ttcgcctcct cgccgatact catcaatccg
     3001 gacttgacct gtccgccctc agacatgttc actccatatg gattgggcgc cagcggcgcc
     3061 caatcgttca gcacggatgt cggctgaagg ccacacctgc gctactcggc aggtgcggtg
     3121 ccagacttct tctcgacgcg gccttccgat acctcagtct tgagccattt gcggtggtgc
     3181 cgccagcccc aaccacccga gaccgtgccc cgcgtcatgg cgctgatcgt gccacggacc
     3241 cagattgctg catagacgtg cacgatccag atcgtaatgg ccgcgaccgc cgcgatggcg
     3301 tgcaggagcg cggccatgcg cttctgatcc atcgtcgcct tgaagctgaa ggtcgcctcg
     3361 gcccatttga ggccaggttc ccaaaggccg aagccggtca ccagcaggat cggcaccagg
     3421 atgaactgga gccagaacac gagcttctgc ccggcattgt acttgccgac ttcaggcaag
     3481 tactcgtcgc gaccggcgag tacgtgcttc agtcgtgcga gccaggcgat gtcggtgcgc
     3541 tccggaaggt tggccggcag gaagcgcaca aagagcagaa ggaaggttac tgcaaacacg
     3601 agcccgaccc aggggtggag ccagcgcacg gtggcgccgc ctccaaagat cccagaaagg
     3661 aagaacagac tcggatggaa cagcgacaat ccactcagca ggagcaggac gaaagagatc
     3721 gccatgatcc agtgattgag ccgcgctgat ccggaatagc ggcgcacgtg cacgtcgccg
     3781 cgacctgatc cgctcatttc tttgtctccg ccagacgctt ggcttcagcc tcgtcgtgat
     3841 ccgtcacctc gttcgggccc ttcaggaagt agtgaagggc tccgaccagt gccgtcgcac
     3901 cgatcacggc catgccagcg tacttgctca cacccttcca tgcctcgact agcgggctga
     3961 tccgcggggt ctgcgcgagg ttcgcataga gctgcggctg atcggcgtga tgcagcacgt
     4021 acatgacgtg cgtgccgttc acgccgggcg gattgtaaag tcccgccttg tcgtagccgc
     4081 gcgccttgag caccgtgatg ctcttctcgg catgcgccac catctcggct ttggtgccga
     4141 agctgatcgc atgcgtcggg cagaccttgg cgcaggccgg ctgctggccg atcgcgatgc
     4201 ggtcggagca gagcgtgcac ttgtaggcct tgttgtcgac ctggctgatg cgcgggatgt
     4261 tgaacgggca gcccttttcg caatagccgc agccgatgca gtgctccttc tggaagtcga
     4321 cgatgccgtt ggcgtactgc acgatcgcac cgggggccgg gcaggccttt aggcagcccg
     4381 gatcctcgca gtgcatgcag ccatccttgc ggatgagcca ctcgagattg ccgctcttcg
     4441 ggttctcgta ctccgtgaag cgcatcagcg tccacgtggc cggcgtcagg tcgtgggggt
     4501 tgtcgtaaac tcccgcgttg gtgccgacgg gctccttcaa gttgttccac tcgagacagg
     4561 ccgactggca ggccttgcag ccgatgcact tcgacacgtc gatgagcttg gcaacctcga
     4621 cgccgcgctt cgcaggcggc gctgcggtga ccgtcgccga tttgcggacg acgtcgagag
     4681 gttgaaaagt agccattttt tcctcctctc tcaggatacc gcggggccgg tggcgattgg
     4741 ctcgatgtca accaggaacg ccttgtattc cggcgtctcg atgttggcat cgccgacgaa
     4801 cggcgtcaga atgttggcgc cgaagccctt cttggccgca cccgtgaacc cccagtggat
     4861 ggggatgccg acgatgtgca ccaccttacc gtcgcaggtg atcggcttga tgcgcttggt
     4921 caccacggcc ttggctttca ccgagccgcg gttcgaccac acacgcaccc aaccaccctt
     4981 gatgatcttc ttctccttgg cgagctcctc cgagatctcg acgaacagct ccggctgcag
     5041 cgtggcgttc accgagacgt gcttcgtcca gaagtggaag tgctccgtca ggcgatacgt
     5101 cacggcggcg taggggaagt ccttggccgt gccgaacacc tccatgtcgc ccttaaacac
     5161 gcgcgcggcc gggttgcccc gcactttggg ggcgatcggg ttggcgatcg ggctctcgaa
     5221 gggctcgtag tgtactggga acggaccgtc cttcatgagg ccgcgcaccc agagccggcc
     5281 gacaccttcc tcggtcatga tgaagggcat cacgacgtcg ggtttggcgg tcggagcgat
     5341 atccgggacg tcgtagccgc tccagcgcgt cccgtcccat gagatgagcc ggcgcttggg
     5401 atcccaaggc tggcccttca tgtcggcgga ggcgcgattg tagaggatgc ggcggttcgc
     5461 gggccacgag aacgcccagg tctggtagga gccggtgccg tcgggatcac gcgtatcccg
     5521 gcgcgccatg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnc gaaaccggcg acttgcttgc ccttctcgag cagcttcttg gtcgggtcgg
     5641 cggggtcggc gacgtcctcg agcacgtagc cgttgatctc cttggcgatc tcggccggat
     5701 gcgggtcttc ggggttcttg tagggccagt gcagggcctt gatggcatcc ggaaatgcgc
     5761 caccctcctt ctcgtagagc gccttgatgc gcttgtagag ctgcgccatg atccagatgt
     5821 cggtcttggc ctcgcccggg ggcgagccgc cggcccaatg ccactggagc cagcgcgagg
     5881 agttggtgag cgatccctcg tcctcggcga acgtcgtcgt cggcagctcg aaaaccgccg
     5941 tcttgatgtc ctccggcttc gatggattgt actcgccgtg gtcctcccag aagcgcgccg
     6001 tctcggtctg cagcggatcc atgctgacca ggaacttcag ctttgcgagc gccgtcttga
     6061 tcttcgcctt gttggggaag gacagcagcg ggttgaagcc ctggcatatg tagccgttca
     6121 tctgtccgtt gtgcatcatc tcgaatgcac gcaggacatc gtaggccggc acgtcgagct
     6181 tcggcaggta gtcgtaggca aagctgttcg ccttggtcgc cgccggcccg tacatcgctt
     6241 tcaggaagct cacgaagaac ttgtcgtagt tctgccagaa gctcacctga ccgggtcgca
     6301 tcggcttaaa gccgcgcttg ccgagatagg tcgcgatgtc gccgtcggcg tcgaccggca
     6361 gcgtgaggta gcccgggatc aggttcgaca tcagcccgag gtcggtgaga ccctggatgt
     6421 tcgagtgacc acggagcgcg ttcatgccgc cgccgcgcac gcccatgttg ccgaggatca
     6481 gctgcagcat ggccatgcag cggatgatct gcgacccctt ggagtgctgc gtccagccga
     6541 gcgcgtacat cgacgtcatc gccttgtcgg gcgccgacgt ttccgagatc atccgggcga
     6601 ccgtgacgaa cttgtccttg ggcgttccgg tgatgcgcga gaccatgtcc gcgttgtacg
     6661 tcttgacgtg cgccttcaag aggttccaca cgcaacgcgg atgctcgagc gtcgcgtcga
     6721 ccttgacgaa gccgtcatcg cccgtctcgt agacccacga gccgcgattg tagtcgcgtt
     6781 tggcctcatc gtagcccgag aacaggccgt cctcgtactt gtagccttcc ttcacgatga
     6841 aagcggcgtt ggtgtagttc ttcaggtact cccactgcac cttgttgttc tcgatgcagt
     6901 gattgatcac gccgagcagg aaggcaatgt ctgagccctg tcggatcggc gcatagaagt
     6961 cggcgaccga cgccgagcgc gtgaagcgcg ggtcgaccac gatcagcttg gcgccgcgcg
     7021 tgtggcgcgc ctcgatgaca cctttgaagc cgcagggatg ggcttccgcg gcattgccgc
     7081 ccatgatgag aacgagatcc gtgttcttga tgtcagtcca cgaattcgtc attgcaccac
     7141 ggccaaacgt cggagccaaa ctggccaccg tcgggccgtg tcaaacacgg gcttggttgt
     7201 cgaacaccac cattccggtc gagcgcacga ccttgtaggt caggaacgcc gtctcgttcg
     7261 tcgtggcgga cgcggccagc atgccggtcg tcgtccagcg gttgacggtc gctccgtcct
     7321 tgttcttggc gatgaagttc gcatcgcgat cggccttcat cagcttggcg atgcgatcga
     7381 gtgcatcatc ccaggagagg cgcttccact cgcgcgtgcc gggctcgcgc accatcgggt
     7441 acttcgtgcg cgtctcggac ttcacgaagt cgaggagggc tgcgcccttc gggcagagcg
     7501 tgcccttgtt ggtcggatgg tcgctgtcgc cctcgatatg gacgatctcg gcagtctcgc
     7561 ccttcttgag atcgcccttg ctgtaaagga tgataccgca gccgacggaa caatagggac
     7621 aggtattgcg ggtctcagtg aggcgcgcga gcctgaaggg tctgatcgaa gctgcaagtg
     7681 cttgctctgc tccaccaaat ccgaacgcac caagcgccgt tccagcaaca cccgctcccg
     7741 acgccttcat gaaggcgcgc cgtgagacag gagcattcat gatgcttgtc ctcctcctgg
     7801 ttcgcgtttt ttttccgtga tgaaccgcgc gcacagtaac acttgcgcat cgatcgtcaa
     7861 tttgcactgc ggtattcgat agttcgaatg ctcagcccgc gaccagtacc gctccatcct
     7921 tgctctgact gaacgaagtt ttcggatttt caatctcttg caagagcact ttaggttcca
     7981 agcccgacgg ctcccgtgca attccgatga ttgctgcatc gcaataactc gctgccatta
     8041 gcgagcgaat gcacccttcg gcctcgctcg ccggcatcgc gaataagagc ccgcccgcgg
     8101 tctgcggatc gaacaggatc cgatagcgcg gccccgagcc gacaccaggc ctcgactcga
     8161 tggcgcgctc caccctcacg ttttcgggag cgatgctgct ctcgatcccg cgaccgatca
     8221 cgtcgagcgc gcccgcgagt accggcagct tgccgaggtc gatagtggcg tcgcagcgcg
     8281 aggccccgag catctcgaga aggtggccgg caagcccgaa cccggtgaca tcggtgcagg
     8341 cggttgcccc cgcgtcgatc gcgattttcg cggccttggc attgctctgc tgcatgacct
     8401 tgagagcggc cgcgatccag ccggcttcag ccgcgccacg catctcggca gcgaacagtg
     8461 cgccggtccc gagcggtttc gtcaggatga gtgcgtcgcc cgggcgaacg ccgcccttgc
     8521 gcaggatccg gctcggatcg acagccccat tgatcgcaaa tcccagcgcc agctcggcgc
     8581 cttcggccga atgtccgccg accagcgcac agccagccgg ctcgaggacg tcgagcgcgc
     8641 cacgcagcat ctggtagagg tcgtcctcca ccatggcctc ggcggcatag ggaatgactg
     8701 ccgtcgcgag agccgtcagc ggcgcgcccc ccatggcgta gacatcaccc agcgcatgca
     8761 ccgccgcgat gcggccgaac aggtagggat cgtcgatgaa ggcgcggaaa aaatcgaccg
     8821 tctgcacgag gtgcatcccc ggtggcggcg cgatcacggc agcatcgtct gcctcgccca
     8881 gcgcaatgac agcgccagcg cttgccttgg gcgccagtcg cgcaagggca cgtgacaaag
     8941 cgttcgcgcc gaccttggcc ccgcagcctc cgcagcgcag cgatgccaac gccgcggcac
     9001 caatgtcgtc tccggctgct ttcgccgtct cgccgcccat gccgggtcgg agcgactggt
     9061 agccatccat ccagcgccgg tcgatccaat ccttgagtgt ccagagccta ggccccgaaa
     9121 gggcgagcgc gcctcgcgaa gcgaccgcca ccttgtcgcc cgtcgagatc agcgacagga
     9181 aattcgtctg cggcttgaag ggctgcagct cgctgccggc gagcgcgcgc cgcaggttct
     9241 cctggagcgg cggtccctgg cgcaccgcga acacgccggc cttgggtctc gggtgaacgg
     9301 tgctcgacgc aatatcgcct gcggcaaaca cgccgggcgt gttcgtcgat tgcaggcatg
     9361 cgtcgacgga aatgaacccg tggccgtcga ttgcgagccc ggtctctcgg agccagggag
     9421 ctgcaccggc atccgtcacc cagaggagct cgtcgaacgc gaacgcgcgg ccgtcctcgg
     9481 tggtgagccc attccgcccg acctcggcga cggcgcagtg ctcgtgtagg gcgacattgc
     9541 ggttccgcag ctcacggcgg aatgccgtcc gtacccgcgc gttgtggctg gcgagcagtt
     9601 cgccgcgcgt caggagcgtg aacgcatgat cacggacctg cgccttggga agcgacttca
     9661 gacgatgttg cgcggcgagc gtcagctcca cgccggccgc tccgccgccg acggtcaaga
     9721 attgcaaggg gcggggcgcg gcctcggcga tttcgagaac tcgccgccaa tgccgcgcca
     9781 ggccgtcgat cggtttcacc ggcgtcgcat gctgccgcgc gcctgcgatc ccgtcgtagg
     9841 tcggcgtcga gccgatatcg atcgagagca cgtcgaaggc gatatccggt cggtcctgga
     9901 cgcggatgcg ccgcgccgac cgatctatgc cgatcgcccc gcccgcaatg agccgggcac
     9961 ctgcgaagcg cgcgagcgga cgcaggtcga tgtggcattc agccttcgtg tagtgccccg
    10021 cgatgaagcc cggcagcatc ccggaatagg gcgtctcgat gtcccgcgac agcagcgtaa
    10081 cgcgcacgcc cgggatcggc ttcatgccga agctcttgag gacatgcaca tgggcatgcc
    10141 ccccgccaat cagtacgaga tccttcagga tcggcgtgac acgcacgaca gcgatcgctc
    10201 catgtctgag ccccgtccgg cgtttccacc tgccggcggc atgtggcccc gcgatcccag
    10261 gctgcttcgg catcgtgtgt cgcagagttt cgggccattg agaagcccta cccgcgcaca
    10321 cgaccgtgat cgcacaacac tttcgcgcga cgacgcctca ccgaaacccg attggggcgc
    10381 taacgttgaa ggtgcccggg atgcgacgcg cacgaccatg aggcgcgccg gcgaaagccc
    10441 ggtgactgga ggagcgtcat gcgtttttca cttcgacttg gggccggcat tgccgccctc
    10501 accgggttcg cgaccgcggc catggctgtg gagccgacgg gcaactgggt gcgcccgtcg
    10561 agcggtacgg tcatcaactt ctatgcctgc ggcgccaatc tctgcgccaa gatcgtctcc
    10621 gtgaaggacg cgagcaagaa gggcacggtc ggcaccgtga tcatgaatgg cgcggtgaag
    10681 tccggcgcca acgtctggaa gggctcgctc ctcaataccg aggacggcca gacctacacc
    10741 ggatcggtga cactctccgg cagcggcctc gttctctccg gttgcgtgct cggcggcctc
    10801 atctgcaaga acgagacgct gcagcgcgtg aaatgagtac cgcggcggcg cgcctcccgg
    10861 gtcatcgcct ccacctccag cacgggccga tcgatctcgt gatcggagcg ttcggaccgg
    10921 ggggtgaggt cgagcgtgcc tacgccgccg ctgaagcgcg ttttccgaga gtgctgccgg
    10981 agctcgtcga ggagcttcca gtgctgcgcg cgccgatcgg cgatgctcgt ccggtcgtcg
    11041 ctggcgagat tgcgcagcgt atgctcacgg ctgtgtggcc gtatcgggac cgcttcataa
    11101 cacctatggc ggcggtcgca ggtgccgttg ccgaggtcat gctcgaggtc atgggcaatg
    11161 ccgccgcact cgatcgcgcc tacgtcaaca atggtggcga cattgcaatc catctggcat
    11221 ctggccatgc catggcgatc ggtgtcgtcg gcgaccttgc cgatccggca atcgatggcc
    11281 gggtgacgat cacggcggac gatcgcgtgc gcggcatcgc cacgtcgggc tggcgggggc
    11341 gctcgctctc gctcggcatt gccgatgcgg tgaccgtgct cgcgacaacg gcgtcccgcg
    11401 ccgatgcggc ggcgacgatg attgccaacg cggtcactgt cgaccatccc gcgatccgcc
    11461 gcgcgcctgc tcgcagtgtg cgcagcgaga gcgatcttgg cgacatgccc gtcacggtcg
    11521 acgtggggga gttgcccgcg gccctgcgcc gaagggcgct cggcgccggc caacgccgcg
    11581 cagaggagtt gatcggcgcc gggctgatcg aggctgctta cttgcgcctt caaggtcaga
    11641 gcattgtcgt cgaccacggt cgagacaccg cgcgccggct tgtggatgga caagcccgcg
    11701 gtgccatgtg aaggtgacca ccccggcacg agcgatgatc agtcgggcga ggggtcgttt
    11761 ggcgggcggc gttcatggca tgtggttgac gtgtcaagcg cttgacaagc gtgaccgcgt
    11821 cagtcatttg acgtgccatg gtcaacaccg ccctcgctct gcgacgtcgc cgttcgtgca
    11881 atcttgcgcg gatgcgatag cggctgtctg accgtttctt cgccgagccg cgccaagtgc
    11941 gcggcttttt tgtttttaga cctcaaaggg gactacccct atggcagtga cagcatctcc
    12001 ctcgcaaggc agcaccaagg cggcgacggc cgccacccgc agcggcccat ccccagcggt
    12061 gatgggcacc tatggccgcc aggatatcgt cttcgagcga ggcgagggca gctggcttgt
    12121 ctccactacg ggagagcgct atctcgattt cggctccggc atcgccgtga acctgctcgg
    12181 gcatgctcac ccgcacctag tgaaggggct cgccgcgcag gcagagaagc tctggcatac
    12241 gtccaacctc tatcgcgtcg cgggccagga aatgctcgcc gagcggctgg tcgcggccac
    12301 gtttgccgac aaggtgttct tctgcaattc gggcgccgag gcctgcgagg gtgcgatcaa
    12361 gctcgcgcgg cgttatcagt atacaatcgg caagcccgag cgctggcaca tcatcacctt
    12421 caagggtgcc ttccatggcc gcacgctcgc gaccatcgcc gccgctggca atgagaagta
    12481 cctcgagggt ttcggccagc cgacgccggg cttcaaggtg ctgcccgagg tgaatgatct
    12541 cgcagcggtc gaagcggccg tcacgcccga ggtcggcgcc atcatggtgg aaccgataca
    12601 gggtgagggc ggcatccgct cgtgcacgac ggagttcctg aaggggctgc gcgccatttg
    12661 cgacaagcac ggcttgttgc ttgtcctcga tgaggtccag tgcggcatgg gccggaccgg
    12721 caagctcttc gcgcacgaat gggccggcat cacgccggac gtcatggcga tcgccaaggg
    12781 tatcggcggc ggcttcccgg tcggcgcgtt cctcgccacg gagcaggcag ccaaaggcat
    12841 ggtaccaggt acccacggct cgacctttgg cggcaatccg ctggcaatgg cggtcggcaa
    12901 cgcggtgctc gacgtcgcgc tcgagaaggg cttcctcgat ggcgtgcaga cgcgggcgct
    12961 ccgcttcaag cagggcctcg cgcgcctcaa agacgagaat ccggacgcga tcgaggagat
    13021 tcgcggccag ggcctgatga tgggtctcaa gctgaagccc gcggtcgccg tcgccgatgt
    13081 cgtcaaggca tgcatggccg agaagctcct catggtctcg gccggcgaga atgtcctgcg
    13141 cctgctgccg ccgctgaacg ccaccgagga tgagctcgcc gaggcgacga aacgcatcgg
    13201 cgcagccctg aagtccgttg caaagccgag gacggcatga gcgagcgcag aaccacaacg
    13261 tcgatcacga ccaaagccag cggcgcgaaa gcgccgcggc acttcctcga tctcgaccgg
    13321 atcgatgcca aggcgctccg tcgcatcgtc gacatgggcc acgccatgaa ggccgccggc
    13381 aagcgcacgc ccaaggcgct gcgcccggcc ggcatcgagg accaggtcct cgtcatgatc
    13441 ttcgagaagc cgtcgacgcg tacgcgcgta tcattcgacg ttgccatgcg tcagctcggc
    13501 ggccagacga ttgcgctcaa tcatgccgat ctgcagacca atcgcggtga gccggtcgag
    13561 gacacggcgc gggtgctctc gcgctacgcc gacgccatca tgatccgcgc gaacaagcac
    13621 gagacgctcc tcggcctcgc cgagaacgcg agcattcccg tcatcaacgg cctcaccgac
    13681 ctctcgcatc cctgccaggt gatcgccgac ataatgacgt tcgaggaggg cgggcgtggc
    13741 gcgatagcgg ggcgcaccgt cgcctggctc ggcgacggca acaacgtcgc cgcctcgtgg
    13801 atgcatgcgg ccgttaagtt caagttcaag ctgcgtctcg cctgcccgcc ccaactcaat
    13861 cccgagaagc aggtgctcga ttgggtgcgg cgcgaaggcg gggacgtcga gctcagtgat
    13921 gacccgcagg ccgccgtcaa ggccgcagac tgcgttgtca ccgacacctg ggtgtcgatg
    13981 ggccagaccg atgcgccgcg cagaaagcag ctgctcggcc cctacgctgt cgacgacgcc
    14041 ttgatgacga aagccgccaa gggcgcgatt ttcatgcact gcctgccggc ctatcgcggc
    14101 cacgaggtct catcctccgt gatcgacggg ccgcagtccg tcgtcttcga cgaggccgag
    14161 aaccggctcc acgcgcagaa ggcgatcctc gcctggtgcc tgggggtcga ctgatgatgg
    14221 gcggggcgag cgccgcgcgg ggcggaaccc tgccgtcagt tccggacgac ctcgccttgc
    14281 cgttccgcac cgtcgcctcg gacgtcgcgg gccggctcgc gcggctcggg ccggcgatcg
    14341 acaccgtgct gacgcgacac gactaccccg agcccgtggc gatcgcgctc ggcgaagcgt
    14401 tgctgctggc ggccctcctc ggcaatccac tgaaggatgg gggccgtctc atccttcaga
    14461 ccaagtcgga tggcccgctc ggcctcctcg ttgcgaacta cgacgcgccg gggcgcctca
    14521 ggggctatgc gagcttcgac agggagaagc tcgcgacgct cgccgccggc ggcccggtca
    14581 gccaggcgtc gctgctcggc tccgggcatc tcgcgctgac gctcgatccg ggcggcgagc
    14641 agacgagcta tcagggcatc gtcgcgctcg aggccggccg gccgggggaa gccggctcct
    14701 ctgcactcgc ggatgccgcg cacgcgtatt tccgccagtc cgagcagctg ccgaccttca
    14761 tccggctcgc cgtcggccgc cagttcctca aggggggcga cggccgcgag cccggctggc
    14821 gctggcgcgc gggcggcatc atcgtgcagc acgtacccaa agagggcggc gacgagcgcc
    14881 ggggcaagct gctcgacgac gaccggctcg acggcgagga cgacgacaac tggcggcgcg
    14941 tccgcatcct gtccgagacg gtctcggcgg aggagctgat cgacccctta ctcgcgcccg
    15001 agagtctgct cttccgcctt taccaggagg agggcgtgcg cgtgacgccg gcgaccgcgc
    15061 tcgaggcgcg ctgccgctgc tcacgagagc gcatcgcgac ctttctgcgc cgcttccagg
    15121 gtgaggagcg tacgagcctc gccgaagccg acggcctcat caaggtcacg tgcgagtact
    15181 gctcgagcaa gtatcagttc aagccggccg agctttgagc gccatcagac cgcgatgccg
    15241 gacgacagca gtggatctgc cggatcgagc ggcatttcca gtttcagccc cgactggttg
    15301 ccgcgcgtcc aggcgatgcg gcccgacagg cggcgcccat ccttgagcct caccgcgacc
    15361 tcctggacac gtacgagccc cggcgcatcg cagatcccga gaccgcctat cgacgcatcc
    15421 gagatcgtga cgggatgaat ggcatctccg atcacgagcg tcgccgtctc ggcgagtggg
    15481 aagcgctggt gccggcggcg ctcggcccag ccaggaatcg agatggcacc gtcggcgtcg
    15541 atgcaatcgg cgtgcccggc ctccgcacgt gcgagcagcg agctcagatg cgatgcgtgc
    15601 tcgcgatcga cctcagtcaa cccggctgtc gcctcgagca cggcgattgc cgtctcgcag
    15661 agactgcgcc agccgtccgc cagaatatcg atggcaaccc ccgagcggcc cggtgtccct
    15721 tcacgggcgt cggcggcaaa cgcgcggctc atgcggcgga gaagatcgag ccggatttcg
    15781 aaggcccgca cgtcggcaag cagctcgata ccgggctcgc tcgcgagaag cggggccgcg
    15841 acatgcgtga cgagcgccag ctccagcggc agatatgtgt cgatcgactt tgcgagcgac
    15901 cgcccctgcc ggccagaggc aatcgccgca acgaccgacg ccgacacggc aaccagaaca
    15961 gccacggcct cgagctcggc ccggatgagg tcgacggcgc gccgcgcctc cgcgccaagg
    16021 cccggcggaa tctcgcggcc gtcgcgggcg cgggcgactt cagccaaggc ggcgccatcc
    16081 aggcgaacga gggtttcgag gtagttcatg gcggcccctt aaccagaaag gctcagccgc
    16141 cagcgtgggc agacaatggt tagggccgcg taaacggcgc tacttgatgc gcacggtcac
    16201 gcggtcggtg cggccacgcg cgtcgatgac cgtgatgcgg gcgaaacctc gaccggctgc
    16261 gagccactcg atctcgtgcg actgcgccgg ctcggcgatc ggctcgccgt cgatcagcca
    16321 ggtgagtggc agcgcgccac cctcggcctt gagcgccagg cgcaagtcct cgcccggctc
    16381 catttcgagc acggagcggt ccggagggaa ggcgatctgg acacccgggt cgagataggc
    16441 gccagcgcgc gcctcgtcgc ccgcctcctt gaacctacgg agcggcattg gcagctgcgc
    16501 tccggaggcg atgattgcgc cgcccggggc cgttgccagc gggacgcggc gcggaccgag
    16561 tgctgcaaaa gcatcgaaga gcagaggcgc cgccgccgtc cgtcccgtga ggcccggagt
    16621 ggcggtcgcg tcgggccggc cgacccagac ggcgatggtg tagcgaccgt cgaagccgac
    16681 ggcaggaagc gcgtcctgcg cgtcggtcag aaggtg
//
LOCUS       DAORNH010000068        16671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2635_length_16671_cov_2.631801, whole genome shotgun sequence.
ACCESSION   DAORNH010000068 DAORNH010000000
VERSION     DAORNH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16671
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2635_length_16671_cov_2.631801"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PK264_09110"
     CDS             <1..946
                     /locus_tag="PK264_09110"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRD76086.1"
                     /translation="VPGMTVNRFCSSGLQTIALAAQRIKAGEADIIAAGGLESISLVQ
                     NEHANKFRGKDDWVAEHKPGIYWPMIQTADMVAKRYNVSRQAQDEFALESQRRTAAAQ
                     QAKRFDAEIVPLPAEKLLFDKEGKVTGKEMFTLTRDEGNRPETNMEGLVKLKPVMGEG
                     NFITAGNASQLSDGASAAVVMSDAMAAKKGLKPLGIFRGFAVAGCEPDEMGIGPIYAV
                     PKLLKKHGLTIDDIGIWELNEAFASQVVYCRDKLGIPGDRLNVDGGAISVGHPYGMSG
                     ARLTGHALIEGKRRGAKYAVVTMCIGGGQGAAGLFEIV"
     gene            1187..1639
                     /locus_tag="PK264_09115"
     CDS             1187..1639
                     /locus_tag="PK264_09115"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD76087.1"
                     /translation="MDPSPTNWHQRIVGNLVSALDAACDVANPIWIAIPGTGTRVPAS
                     SFSLPQPDVMVVPALTETATPVSDDALAISEVLSRSNTESDQPWRRQVYASVPSCRHY
                     VTIDSRNVAVERFDRASEWKRTAHLALLDDALALDGLGITPRIADLYR"
     gene            1856..2485
                     /locus_tag="PK264_09120"
     CDS             1856..2485
                     /locus_tag="PK264_09120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD76088.1"
                     /translation="MAPGYHHRGIDKLFEIAARTPIRVGVDRFVFVRHGETDGNKNKI
                     FQRSDQPLNAAGLAQAEAARVILASERIAAIKASSMDRAFVTASIIGKPHGLAPSPVD
                     DLRERWFGDLVGTPAGGYDWTNAPPNGETLATFVTRTQRGLAASLASHDDTAIVAHGG
                     TLYVIGPSLGIEIAPEHYANATPLRFERAGGSWRVTALGSSGGKVSAVT"
     gene            2542..3312
                     /locus_tag="PK264_09125"
     CDS             2542..3312
                     /locus_tag="PK264_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRD76089.1"
                     /translation="MAKKSIRQRVLASETVLGSMVFEFFVPGVAQCLKHGGAEYVIFD
                     MEHAGLGLETLKMLVATSRGIGIAPMVRVPRGEYHFIARALDVGAQGIMVPMVESVAE
                     AKRLAEAMRYPPRGRRGAAFGFAHDNYEPGDPAAKMRAADARNLAIAQIETERGLEAV
                     DEIAAVDGIDVLWVGHFDLTNFLGIPGQFDHPKYVDAIKRVVAAGRRHKKGLGFMAAD
                     TKWARDYKRLGFNMLATGTDHGLLMSGVRAILESVEDK"
     gene            3312..4319
                     /locus_tag="PK264_09130"
     CDS             3312..4319
                     /locus_tag="PK264_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76090.1"
                     /translation="MTFRIGITRDLLTRSGEPCFDPRALDVLEGVDGLTWEWVPEDFA
                     VLPPEVAARYDGLHLNLPKVTAESVGRQDCRLKIIARNGVGYDSVDVAACTAKGIAVT
                     NTPVAVRRPVAVATLTLIFALAGRLFAKDRLVRAGRWNDRTDFMGQGLTSRTLGLVGA
                     GSIGQEIMRLARPFFGAMIAADPFVAASAVEPLGARLVPLDQVLSEADFVVVCCLLTK
                     ETHHLIDARALSLMKHSAYFINVGRGPIHDEAALVDALRAGAIAGAGLDVTEVEPISP
                     SSPLIAMDNVILTPHALCWTDECFRDIAETALRSLVSVARGERPVHVVNTNIYATARK
                     S"
     gene            4443..4655
                     /locus_tag="PK264_09135"
     CDS             4443..4655
                     /locus_tag="PK264_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76091.1"
                     /translation="MSQSNNEIPEDVFQTEFGGRSFVDQKNEQRGEARTVLAFWGLLA
                     LIGGPAVLVAVAVIIVSLKDCWAKMC"
     gene            complement(4677..5912)
                     /locus_tag="PK264_09140"
     CDS             complement(4677..5912)
                     /locus_tag="PK264_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRD76092.1"
                     /translation="MTTCRAERAVEDLRALARIGAYETGVHKPSLSAEHVKSLDWFAG
                     RAKAAGLDVTIDGIGNVMAVTPGAGPRLLTGSHLESQNHAGWLDGPLGVIYGLEAARA
                     ILEDQRLKGSGIGVDVGVWCDEEGHFGHFLGSRSAVGEVSEADIDAASDRTHGTPMRR
                     MLADAGFAGRQRFVIDPKRYVGYLEAHIEQGDWLEANHLKIGVVTSIVGIRQYKVTFE
                     GAQNHAGTTRMAIRRDAGLACARLCVAIDETFPKFAGERTVWTTGRITLDPGGPSIIP
                     GRAEMLFQVRDADAAVLDRMEAALGELVTASDRRGPCRAHLERLRASRPAIMDQRIMG
                     AIEAATAAHVPGKSTRIPSGAGHDAQVFAGHMPAGMMFVPSIGGISHHWTENTSDEDI
                     MIGAKVFVDACVGLLTAKV"
     gene            complement(5909..6595)
                     /locus_tag="PK264_09145"
     CDS             complement(5909..6595)
                     /locus_tag="PK264_09145"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRD76093.1"
                     /translation="MTHLRSPLGFGSMRVRPPLTGPGQRIGLLGGSFNPPHDGHRQIS
                     VTALKRLGLDAVWWIVTPGNPLKSTGELATLASRMAEARGRVRHARIKVTDFEAGLPS
                     AYTAATLAFLKRRHPGVCFVWLMGADNLVSIHRWRNWETIFRSMPIAVLDRPGWRLKG
                     LVSKAARRFRAGFVPEAGAQRLASTRAPAWTFISLPLSPLSSTEIRRQRRTFLKARSG
                     ASVAATGELP"
     gene            6892..7425
                     /locus_tag="PK264_09150"
     CDS             6892..7425
                     /locus_tag="PK264_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRD76094.1"
                     /translation="MPLYELDGHRVTTPGAGRYWVAPGASVIGRVRIGEDVSIWFGAV
                     IRGDNEPIDIGARTNIQENCVLHVDPGFPMLIGEDCTIGHLAMLHGCTIGKGALIGIG
                     TKVLNGARIGAGALVGAHSIVGEGKEIPPNALAVGSPAKVIRELTPKDRERIAAGVTD
                     YMRRWRHYSAKLVVQPE"
     gene            complement(8289..9716)
                     /locus_tag="PK264_09155"
                     /pseudo
     CDS             complement(8289..9716)
                     /locus_tag="PK264_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     assembly_gap    9717..9743
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9744..11053)
                     /locus_tag="PK264_09160"
     CDS             complement(<9744..11053)
                     /locus_tag="PK264_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD76095.1"
                     /translation="MAKITFELDGKAVEADDGETIWQVAKRHGTTIPHLCWHPGPGYR
                     ADGNCRACMVEVEGERVLAASCQRKAAAGMKVRTATERAKKSRQMVFELLLADQPVRG
                     DSADPASKFWSWIDDMGIATTSRLPRAERPASDRSHAAIAVNLEACINCGLCVRACRE
                     VQMNDVIGMAYRGSHAKVVFDFDRGMGESTCVACGECVQVCPTGALMEANLVDAGTQR
                     RVAFETRTVDTLCPYCGVGCQTKVHVKDDKILYVDGRDGPANNNRLCVKGRFGFDYIH
                     HPDRLKTPLVRRDDVAKDANMQLARGEWQSVFREATWEEALERAAGGLKAIYSREGGK
                     ALAGFGSAKGSNEEAYLFQKLIRQGFGTNNVDHCTRLCHASSVAALMEGLNSGAVTAP
                     FTAAKDADCIIVIGARPAENHPVAATFLKNAAKRGAKLVVMDPRG"
     gene            complement(11097..11630)
                     /locus_tag="PK264_09165"
     CDS             complement(11097..11630)
                     /locus_tag="PK264_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76096.1"
                     /translation="MTMTVLAFCLAFMAVGAVGATRALAAEPVGRSRLLARTPQEVLE
                     LEIGRSTTIERLGELSGLPSPHVLAENVAHLSLAPSQHDKLAALRKASDASTRAAGRS
                     VIDAELSLERAFSTGPPQPVTLARLVRRAEEASGDLRIVILQSMIDCERILTAAQLRR
                     YFELRRLQPRMIRSIDA"
     gene            complement(11627..13345)
                     /locus_tag="PK264_09170"
     CDS             complement(11627..13345)
                     /locus_tag="PK264_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRD76097.1"
                     /translation="MSGEGAGAASRAGSGRRVHPGAGRRSAPLAPKGRVLRQSELDGV
                     VRLIGPGPYERALLIEYLHKVQDAEGCLPAGCLQAVAELLKISMAEVFEVATFYAHFD
                     VVMDDEALPAPTVIRVCDSLSCMLGGGDALLAALSAVPPPGTRVVRAPCLGACHHAPA
                     AHVGHVTVHRATVESIVAVAVTDTHDAPIPAYKSLDAYRAEGGYELLEACLEGRRTVD
                     DVILQLSDGGLRGLGGAGFPTGRKWGLVRSAKGPRLMAVNGDEGEPGTFKDRHYLETN
                     PHQFLEGLAIAAWAVEAEEVYIYMRDEYPAVIDILKREIAALEAAKILPTGAVRVRRG
                     AGAYICGEESAMIESIEGKRGLPRHRPPYVAQVGLFGRPTLVNNIETLYWVPLILKNG
                     SEWFARHGKNGAKGPRSYSVSGRVKSPGVVLAPAGSTARDLIEACGGMLDGHHFKGYL
                     PGGASGGILPAYMSDIPLDFGQLEKHGCFVGSHAVVILSHHDDMKAVALNLMKFFEDE
                     SCGQCTPCRNGTEKAVKLMQSDEWDVGLMGDLAQVMSDASICGLGQAASNPVKCVIRY
                     FPEDLR"
     gene            complement(13549..14157)
                     /locus_tag="PK264_09175"
                     /pseudo
     CDS             complement(13549..14157)
                     /locus_tag="PK264_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(14714..15514)
                     /locus_tag="PK264_09180"
     CDS             complement(14714..15514)
                     /locus_tag="PK264_09180"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76098.1"
                     /translation="MEPVAAPQPLLGVRGVTLQYKTKHHLVTATYRVSFDVLQSDRFV
                     LLGPSGCGKSTLLKAVGGYMAPTEGTMTLKGAQIRKPGPDRCMVFQEFDQLLPWKTVK
                     QNVMFALVSSGRMRGKAAEEQALRYIEKVNLAKFANAYPHTLSGGMKQRVAIARAMAM
                     EPDILLMDEPFAALDALTRRKMQDELLQLWEDTRFTVLFVTHSIPEAVLIGNRILLMS
                     PHPGQVKAELNSDGSDPVDPASGQKLSDRIHHMLFSDRVEEQGTVAHV"
     gene            complement(15673..16080)
                     /locus_tag="PK264_09185"
     CDS             complement(15673..16080)
                     /locus_tag="PK264_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76099.1"
                     /translation="MTDKATTFLLTYWPFLAVAVAVAFVVLTRTGRAGALFALRFISR
                     PLLLIAVIALVYDGTRTIAGPGRITVTSLLEHTTTIAPKAVETVQRRIPPIVWEQGVQ
                     RVLRLPAWLVLGALGLGLAWLGRRRQRIDVFIN"
     gene            complement(16171..16419)
                     /locus_tag="PK264_09190"
     CDS             complement(16171..16419)
                     /locus_tag="PK264_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76100.1"
                     /translation="MDVRAELIRALDLAIAGDWEAAHLVVQRYEDDSTGCWIHACLHK
                     IEGDAANSRYWYRRASQFYESYADPKAELAAIKAALTY"
     gene            complement(16398..>16671)
                     /locus_tag="PK264_09195"
     CDS             complement(16398..>16671)
                     /locus_tag="PK264_09195"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HRD76101.1"
                     /translation="TMRALETALSLGMDRRVQIATPLMWLTKAATWALAEDLGGRDLV
                     DLIVSDTHTCYLGERGRKHDWGQGCGSCPACSLRAKGFYEWTSARS"
BASE COUNT         2903 a         5337 c         5560 g         2844 t
ORIGIN      
        1 ggttcccggc atgacggtca accgcttctg ctcgtcgggt ctgcagacga tcgcgcttgc
       61 cgcgcagcgc atcaaggcgg gcgaggccga catcatcgcg gcgggcgggc tcgagtcgat
      121 ctcgcttgtc cagaacgagc acgccaacaa attccgcggc aaggacgact gggtcgccga
      181 gcacaagccc ggcatctact ggccgatgat ccaaacggcc gacatggtgg caaagcgcta
      241 caacgtctcg cggcaggcgc aggacgagtt cgcgctcgaa agccagcggc gcacggcggc
      301 cgcccagcag gcgaagcgct tcgacgccga gatcgtgccg ctgccggcag agaagctcct
      361 cttcgacaag gaaggcaagg tcacgggcaa ggagatgttc acgttgacgc gtgacgaggg
      421 caatcgcccc gagaccaaca tggagggtct cgtcaagctg aagcccgtga tgggcgaagg
      481 caacttcatc accgccggca atgcgagcca gctctccgac ggcgcctcgg cggcggtcgt
      541 catgagtgac gcaatggcgg ccaagaaggg cctgaagccg ctcggcatct tccgcggctt
      601 cgcggtcgcg ggttgcgagc ccgatgagat gggcatcggc ccgatctacg ccgtgccgaa
      661 gctcctcaag aagcatggcc tcacgatcga cgatatcggc atctgggaac tcaacgaggc
      721 gttcgcatcg caggtcgtct attgccgcga caagctcggc atccccggcg accggctcaa
      781 tgtcgacggc ggcgcgatct cggtcggcca tccctacggc atgagcggcg cgcggctcac
      841 cggccacgcg ctcatcgaag gcaagcggcg cggcgcgaag tacgccgtcg tcaccatgtg
      901 catcggcggc ggccagggtg cggccgggct cttcgagatc gtgtgaccac ggctcgcgca
      961 ttggatcgga tgcaatgccg gcgacgcggt ccgggctgtc atcggaccgc gggcgcggcg
     1021 tgctacaaaa agattgccgc cgcgcgccgg tcccctagat ccactcggag ccccgcatgc
     1081 ctgccgctct cgacgacatc ctgagcatga gcattgagga cttcctcgaa ttcaccgaca
     1141 cgcgccccga cggcgagcgc tgggagctga tcggggggtg cccgtcatgg atccgtcgcc
     1201 aaccaactgg caccagagaa tcgttggcaa cctggtcagc gcactggatg cggcctgtga
     1261 tgttgccaat ccaatatgga tcgcaattcc aggcacaggt acacgcgttc ccgcctcgag
     1321 cttcagcctg ccgcagcccg atgtcatggt cgtgccggct ctgacggaaa cggcgacgcc
     1381 ggtctcggac gatgctctcg cgatttccga agtgctgtcg cggagcaaca cggagtccga
     1441 tcagccatgg cgccgtcagg tctacgcgag tgtgccgagc tgccgccact atgtgacgat
     1501 cgattcgcgg aatgtcgccg tcgagcggtt cgaccgtgcc tccgaatgga agcgaacggc
     1561 gcaccttgct ttgcttgacg atgctctcgc gctcgacgga ctaggcatta caccccgcat
     1621 cgcagacctc tatcgctaga cgccgctcgg gcgcccctag tgtcccgatc gcgaagttcg
     1681 ttaccacgtg ccgagagctc ggacgaactt cgcgatcgat aaggacacga gcaatgtcat
     1741 gagcttagta tggcgctatg ttcggacgtt cgaatctcgc gtgtgccgca cagtgaatac
     1801 gaacgtccga accgccacac tggcactgct gccttcaacc agacgaaaag cacccatggc
     1861 acctggctac catcatcgcg gaatcgacaa gctgttcgag atcgcggccc gcacgccaat
     1921 tcgcgtcggc gttgatcgtt tcgttttcgt ccgccatggc gagaccgacg gcaacaagaa
     1981 caagatcttt cagcgctccg accagccgct caacgcggcg ggccttgctc aggccgaggc
     2041 ggcacgcgtg atcctggcat cggagcgcat cgcggcgatc aaggcctcga gcatggatcg
     2101 ggcctttgtg acggcctcga tcatcggcaa gccgcatgga ctcgcgccaa gcccggtcga
     2161 cgatctccgc gagcgctggt tcggcgacct cgtcggcaca ccggccggcg gctacgactg
     2221 gaccaatgcg ccgcccaacg gcgagacact cgcgacgttc gtcactcgaa cgcaacgggg
     2281 gctggcggcg tcgctcgcct ctcacgacga caccgcgatc gtcgcgcacg gcggcacgct
     2341 ctacgtgatc ggcccgagtc tcggcatcga gattgcgccc gagcactatg ccaacgcgac
     2401 cccgctccgc ttcgagcggg cgggcgggag ctggcgcgtc accgcgctcg gctcgagcgg
     2461 cggcaaggtc agcgccgtga cgtgacggca ggaatcttgt cactgcgcat gacatcggat
     2521 cggccatctc ggggaacagc catggcaaag aaatccattc ggcagcgtgt acttgcgagc
     2581 gagacggtgc tcggctcaat ggtgttcgag ttctttgtgc cgggtgtcgc gcaatgcctg
     2641 aagcatgggg gtgccgagta cgtcatcttc gacatggagc atgcgggcct cgggctcgag
     2701 acgctgaaaa tgctcgtggc gacctcgcgc ggcatcggca tcgcgccgat ggtccgcgtg
     2761 ccgcgcgggg agtaccattt catcgcacgc gcactcgacg ttggcgcgca aggcatcatg
     2821 gtgcccatgg tcgagagcgt ggcggaggcg aagcggctcg ccgaggccat gcgctacccg
     2881 cctagaggcc ggcgaggcgc ggccttcggt ttcgctcatg acaactacga gccgggcgat
     2941 ccggccgcca agatgcgcgc cgccgatgcc cgcaacctcg ccatcgccca gatcgagacc
     3001 gagcgcggcc tcgaggcagt cgacgagatc gccgcggtgg atggcatcga cgtgctctgg
     3061 gtcgggcatt tcgatctcac gaatttcctc ggcatcccag gccagttcga tcatccgaag
     3121 tacgtggatg ctatcaagcg tgtggtcgcg gccgggcgac gccacaagaa gggcctcggc
     3181 tttatggcag ccgataccaa gtgggcgcgc gactacaagc ggctcggctt caacatgctg
     3241 gcgaccggca cggatcacgg tctcctcatg agcggcgtgc gcgccattct cgagagcgtc
     3301 gaggacaagt gatgaccttc aggatcggca tcacgcggga tctgctgacg cgctcgggcg
     3361 agccctgctt cgatccgcgc gcactcgatg tcctcgaggg cgtcgatggc ctcacctggg
     3421 agtgggtgcc cgaggacttc gcggtcctcc cacccgaggt cgccgcccgc tacgacggct
     3481 tgcacctcaa tctgccgaag gtgacggccg aaagcgtcgg ccgtcaggat tgccggctga
     3541 agatcatcgc gcgcaacggt gtcggctatg attcggtcga tgtcgctgcc tgtactgcga
     3601 aaggcatcgc cgtaaccaac acgcccgttg ccgtgcgccg tccggtcgcc gtcgcgacct
     3661 tgacgctgat cttcgcactc gccggccggc tcttcgccaa ggatcggctg gtgcgagccg
     3721 gccgctggaa cgaccgcacc gacttcatgg ggcagggtct cacctcgcgc acgctcgggc
     3781 tcgtcggggc gggtagcatc ggccaggaga tcatgcgcct tgcgcgcccg tttttcggag
     3841 ccatgattgc ggccgatccg tttgtggcgg cttcggctgt ggaaccgctc ggtgcgcgcc
     3901 tggttccatt ggatcaggtg ttgagcgagg cggatttcgt ggtcgtgtgc tgcctgctca
     3961 ccaaggagac gcaccatttg atcgatgcca gagccttgtc gctgatgaag cacagcgcct
     4021 acttcatcaa cgtcggccgc ggcccgatcc acgatgaggc ggccctcgtc gatgcgttgc
     4081 gcgcgggggc cattgcgggc gcgggcctcg acgtgaccga ggtcgagccg atttcgccct
     4141 cgagcccgct catcgccatg gacaacgtga tcctcacgcc gcacgcgctc tgctggaccg
     4201 atgaatgctt ccgcgacatc gctgagacgg ctttgagatc gctcgtctcg gtggcgcggg
     4261 gcgagcggcc ggtgcatgtc gtcaacacga acatctacgc gactgctcga aaatcatgaa
     4321 cccccgaacg gtcacccgcg gatcaaccgt tccttaagca acatggcttt acctgggcag
     4381 accggacacg actgggccat gcccatttcg tgaccgaaat cctgcgcaac agggggcaag
     4441 ggatgagtca gtcgaacaac gaaattcctg aggacgtgtt ccagacggag ttcggtggac
     4501 ggagcttcgt cgaccagaag aacgagcagc gcggggaagc gcgcacagtg cttgcattct
     4561 gggggcttct cgcgctgatc ggcggcccgg ccgtgctggt ggcggtcgcc gtaattatcg
     4621 tgtcgctgaa ggactgctgg gcgaagatgt gctgacgcga cgttccgccg tcgccgtcac
     4681 accttggccg tcagcagtcc gacgcacgca tccacgaaca ccttcgcgcc gatcatgata
     4741 tcctcatcgg acgtgttctc ggtccaatga tggctgatgc cgccgatcga ggggacgaac
     4801 atcatgccgg ccggcatgtg gcccgcgaag acctgcgcgt cgtggcccgc gcccgaggga
     4861 atgcgcgtcg actttccagg gacgtgcgcc gcggtcgcgg cctcgatggc tcccatgatg
     4921 cgctgatcca tgatggccgg tcggctcgcg cggagccgct cgaggtgggc gcgacagggg
     4981 cccctgcgat ccgacgccgt gacgagttcg ccgagcgccg cctccatccg atcgagaacc
     5041 gcggcgtcgg cgtcgcgcac ctggaacagc atttccgccc ttccgggaat tatgctcggt
     5101 ccgcccggat cgagcgtgat gcgacctgtc gtccacaccg tgcgctcgcc ggcgaatttc
     5161 gggaacgtct cgtcgatcgc cacgcagagc cgggcgcagg cgaggccggc gtcacggcga
     5221 atcgccatgc gggtcgtgcc ggcatggttc tgagcgccct cgaatgtcac cttgtattgc
     5281 ctgatgccga cgatcgatgt gacgacgccg atcttcaagt ggtttgcttc gagccaatcg
     5341 ccctgctcga tatgcgcctc gagatagccg acgtagcgct ttggatcgat cacgaagcgc
     5401 tgccggccgg caaatccggc gtcggcgagc atccgccgca tcggcgtgcc gtgggtgcgg
     5461 tcgctggccg cgtcgatatc ggcctcggat acctcgccga ccgccgagcg gctgccgaga
     5521 aaatggccaa aatggccttc ctcgtcgcac cacacgccga catccacgcc gatcccggag
     5581 cccttgaggc gttggtcctc gaggatcgcg cgggccgcct cgagaccgta gatgacaccg
     5641 agcggaccat cgagccagcc cgcgtggttc tggctttcga gatgcgagcc cgtcagcagc
     5701 cgcggacccg ccccgggtgt cacggccatg acattgccga tgccgtcgat ggtgacgtcg
     5761 aggccggcag ccttggcgcg cccggcgaac cagtcgagcg acttgacgtg ctcggccgac
     5821 agtgacggtt tgtgcacgcc ggtctcgtag gcgccgatcc tggcgagagc tcgcaggtct
     5881 tcgactgcgc gctcggcacg gcacgtcgtc atggtagctc tccagtggct gcgacggagg
     5941 ctccgctccg cgccttgagg aacgttcgcc tttgccgtcg gatctcggtc gaggagagtg
     6001 gcgaaagcgg cagcgaaatg aatgtccagg ccggcgcccg tgtcgatgcc aggcgctgcg
     6061 cgcctgcttc cggcacgaag cccgcgcgga accggcgtgc cgccttggag acgagccctt
     6121 tgaggcgcca gcccggacga tcgagcacgg cgatcggcat cgagcggaag atcgtttccc
     6181 agtttcgcca gcgatggatc gacacgaggt tgtcggcgcc catcagccag acgaaacaca
     6241 cgccggggtg acgccgcttg aggaaggcca gcgtagcggc cgtataggca gacggcaggc
     6301 ccgcctcgaa gtctgtgacc ttgatacgag catgacgcac gcgaccgcgc gcctcagcca
     6361 tccgggaggc gagggttgca agttcgcccg tcgacttcag cggattgccg ggagtgacga
     6421 tccaccagac ggcatcgagc ccgagccgct tcaaggccgt gaccgagatc tggcgatggc
     6481 cgtcatgggg cggattgaac gagcctccga gcagtccgat gcgctgaccg gggcctgtga
     6541 gcggtggtcg cacacgcatg ctgccgaagc caagcggcga cctcagatgc gtcacggcgc
     6601 gtctccgaca agcatgacct ttgcctctgt cgagttgtgg cggcccacgt gtccggacag
     6661 agggtgcctc gagcagagta caatcgcggc tcggcgaata tcaactcgca cgttccatct
     6721 ggctgagcgc ggctgagggc gcgcgcgctc ctcggccggc agggcgagca aaggcgagcg
     6781 gggctggcgc tctggaaatt gcccggcatt gaccttgtcg tcgtgcttga ccgcggcttc
     6841 gctgcatggc cacgtcgcgt cctcgacaca tcaagatcgc gaggcccgcg catgccactc
     6901 tacgagctcg atggacaccg cgtcacgacg cccggcgctg ggcgctactg ggtggcacct
     6961 ggggcaagcg tgatcggccg cgtcaggatc ggcgaggatg tcagcatctg gtttggcgcc
     7021 gtaatccgcg gcgacaacga gccgatcgat attggcgcca ggacgaacat tcaggagaat
     7081 tgcgttctcc acgtcgaccc tggattcccc atgctgatcg gcgaggactg caccatcggc
     7141 catctcgcca tgctgcacgg ctgcacgatc ggcaagggcg cgctcatcgg catcggcacc
     7201 aaggtgctca acggcgcacg gatcggcgca ggcgcactgg tcggtgccca ctcaatcgtc
     7261 ggcgaaggca aggagatccc gccgaatgcg ctcgcggttg gctcgcccgc aaaggtcatc
     7321 cgtgagctga cgccgaagga tcgcgagcgc atcgcggccg gcgtcaccga ctacatgcga
     7381 cgctggcggc actattccgc caagttggtg gtgcagcctg aatagtcggg cgcgggtagc
     7441 ccactgcggc atgcaagccg acgctcaaaa agaaagcgcc cagctccggg ggggaggagc
     7501 tgggcgcggc gcttcagata gcgctcacac cgtgagggaa acggtgtgac agcgacgcct
     7561 tcggctagac gggggggact gccgagaggg tggcgtcgac tgcgatgaca tgaagatagc
     7621 gcgccggtgc ccgccgacaa ggggcacccg ccatctcatc acgattattt tacaccgtat
     7681 ttcacagcta cagcccgaca atgacttgat cgtggcgcgg gcgcaccatg agtgctgatt
     7741 tgtcacgaac agatcagcaa cggagccgtc gttgcataga attgccatcg ccgtaacgac
     7801 tgctcgaacc gtgacggatc aagctgattg ggtagtacag cgaacttgct ctccaacgag
     7861 tggttgagct ggcgccggca gtgttccaat cacaagagca atggcagaag gtggaagccc
     7921 ccttccgctg atgctttcga tttcgaggat tcgatgcgcg ccgcttgtgc acgtcaactg
     7981 cgcgtcggca ttccaggaac cgtccgtgat ctcgatgacg acaatggtgc cgcgacttgg
     8041 caatacgaac acgtcgatca cggttccagc accgacgtcc ggcccattgc gatgtctacg
     8101 gtcggtcatg ttgatcttcg cccaccttcc gcgacggcgc ctccaggtaa ttgctgagca
     8161 taggcgtacc tagcatgttg ctcagctacg gggtttctgc ggcgtcgtga acggacgcag
     8221 ccaccagcac cagcctctcg gcggcgtcgc agaaacgata taattacccg acggcctgcc
     8281 tcctactcgg cagcactggc catggtcgtg cgttcgatcc gggccgcgca gtacttgaac
     8341 tcggggatct tgcccatcgg gtcgagctgc gggttggtga gcatgttggc gggcgcctcg
     8401 gcgaaagcga acgggatgaa caccatgccc ggagcgacat cccggtcggc gcggaccttg
     8461 agcgtgatgg cgccgcgccg tgtcgagacg gtgacgaact cgccaggcga gatcgacaat
     8521 cgctcgagct cgttcggatg aaggcagacg accgcctccg gctccagctg atcgagcaac
     8581 gtcgcacgcc gtgtcatcga gcccgtatgc cagtgctcga gcatgcggcc cgtggtcaga
     8641 accatcggat agtcggcgtc cggcatctcg tcgggcgcgc gcagttccgc cggaacgatg
     8701 cgggcgcggc cggaggctgt cgggaaggac tgagcgaaaa tgatctccgc accgggcttg
     8761 tcgggggcct cgcagggata agtgacgcag tcctcgcgaa ggaggcgctc ccaagtgatg
     8821 ttcttcaagg agggcatcac ctgtcccatc tcggcgaaga cgtcggcggc cgatgcgtag
     8881 ctccacggca ggccgatgcg ccgtgcgatc tcctggatga gctcccaatc ctgccgcgcc
     8941 tctcctggcg gggcgacgac cggccgcgcg atctgcacct gccggttggt attggtgaag
     9001 gtgccccact tctcggcgtg cgcagaggcg ggcagcacca catcggcgtg ccaggcggtc
     9061 tcggtgagga agatgtcctg cacgaccagg tgctcgagat gggcaagcgc ctcgcgcgcg
     9121 tgctgcacgt ccggatcgga catcgccggg ttctcaccga ggatgtacat gcccttgatc
     9181 ctgtcgtcgt ggatcgcgtg catgatctca accacggtga ggccacgctt tgaatcgagc
     9241 gccacacccc agaagtcctc gtattccttg cggatctccg ggttctcgac cgatcgatag
     9301 tccggaaaga ccatcgggat gaggcccgcg tccgaggcgc cttgcacgtt gttctgtccg
     9361 cgaaggggat gcagtcccgt tcccgggcgg ccgatctggc cggtcgtcag cgcaagcgag
     9421 atgaggcagc gcgcgttgtc ggtgccgtgg gtgtgctggg agatgcccat gccccagaag
     9481 atgatggagc gctcggagcg cgcatagagc cgtgcgacct cgcggatggt cgccgcccta
     9541 attccgcaga tgccttccat ggcctcgggc gagaactcct tcacgcgcgc cttgaggctc
     9601 tcgaatccct cggtgtgcgc ctggacgtac tgcacatcga ccatgccttc ctcgacgatc
     9661 gtgtgcagca tggcgttgag cagcgcgacg tcgcggcccg gcttgaactg aaggatnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnccgcgcg ggtccatgac gacgagcttg gcgccgcgct
     9781 tcgccgcatt cttgaggaag gttgctgcga cgggatggtt ctcggccggc cgggcgccaa
     9841 tgacgatgat gcagtcggcg tccttcgccg cggtgaacgg cgcggtgacg gcgccggagt
     9901 tcaacccctc catcagcgcg gcgaccgacg aggcgtggca aaggcgcgta cagtggtcga
     9961 cgttgttggt gccgaagccc tgccggatga gcttttggaa gagataggcc tcctcgttgg
    10021 agcccttggc cgaaccgaag ccggcgagcg ccttgccgcc ctcgcgcgag tagatcgcct
    10081 tgaggccgcc ggccgcgcgc tcgagcgcct cctcccaggt ggcctcgcgg aacaccgact
    10141 gccactcgcc gcgggcgagc tgcatgttgg catccttggc gacgtcgtcg cgacgaacaa
    10201 ggggtgtctt cagccggtcc ggatggtgga tgtagtcgaa accaaagcgg cccttcacgc
    10261 agaggcggtt gttgttggcg gggccgtcgc ggccgtcgac gtagaggatc ttgtcgtctt
    10321 tgacgtgcac cttcgtctgg cagccgacgc cgcaataggg gcagagcgtg tcgaccgtgc
    10381 gcgtctcgaa ggcgacacga cgctgcgtcc cggcatcgac gagattggct tccatcagcg
    10441 cgccggtcgg gcacacctgc acgcactcgc cgcaggcgac gcaggtgctc tcgcccatgc
    10501 cgcgatcgaa atcgaacacg accttggcgt gcgagccgcg atacgccatg ccgatgacgt
    10561 cgttcatctg cacttcgcga caggcgcgga cacagaggcc gcaattgatg caggcctcga
    10621 gattgacggc gatcgcggcg tgcgagcggt cggacgcggg ccgctccgcc ctcggcaggc
    10681 gagacgtcgt cgcgatgccc atgtcgtcga tccagctcca gaacttggac gcgggatcgg
    10741 cgctgtcgcc tcgcaccggc tggtcggcga gcagcagctc gaacaccatc tgccgcgatt
    10801 tcttcgcccg ctcggtggcg gtccgcactt tcatgccggc ggcggccttg cgctggcaag
    10861 aggccgcaag aacgcgctcg ccctcaacct cgaccatgca ggcgcggcaa ttgccgtcgg
    10921 cgcgatagcc gggtcccgga tgccaacaga ggtgcgggat cgtcgtgccg tgacgcttgg
    10981 cgacctgcca aattgtctcg ccgtcgtcgg cctcgaccgc tttcccgtcg agctcgaaag
    11041 tgatctttgc catggatttc gcctcgggat gcgagtggtg tcggaagacc tggcgttcag
    11101 gcgtcgattg agcggatcat gcgcggctgg agccgtcgaa gctcgaagta gcggcggagc
    11161 tgggcagcgg tcagtatgcg ctcgcagtcg atcatcgact ggaggatgac gatacggaga
    11221 tcccccgaag cttcctcagc ccgacgcacg aggcgggcga gtgtcaccgg ctgcggcggc
    11281 cctgtcgaga aagcccgctc gagactgagt tcggcatcga tgacgctgcg gccggcggcg
    11341 cgcgtggaag catccgatgc cttgcgcaac gcggcgagct tgtcatgctg cgatggtgcc
    11401 agcgacagat gagcgacatt ctcggcgagg acgtgtggag agggaaggcc gctgagctcg
    11461 cccaagcgct cgatcgtcgt ggaacggccg atctcgagct ctaaaacctc ctgcggcgtc
    11521 cgggcgagca gccgcgatcg cccgacgggc tcggccgcga gcgcgcgcgt tgcgccaacg
    11581 gcgccaaccg ccatgaatgc caggcagaag gcgaggaccg tcatcgtcat ctgaggtcct
    11641 ccggaaagta gcggatcacg catttgaccg ggttggatgc cgcctgcccg agcccgcaga
    11701 tcgaggcgtc cgacatcacc tgggcgaggt cgcccatgag gccgacatcc cattcatcgg
    11761 actgcatcag cttgacggcc ttctcggtgc cgttgcggca gggcgtgcac tgcccgcagc
    11821 tctcgtcctc gaagaacttc atgaggttga gcgcgacggc cttcatgtcg tcgtggtgcg
    11881 agaggatcac caccgcatgg ctgccaacaa agcagccgtg cttctcgagc tggccgaagt
    11941 cgagcgggat gtccgacatg taggccggca ggatgccgcc cgaggccccg cccggcaagt
    12001 agcccttgaa gtggtggccg tcgagcatgc cgccgcaggc ctcgatgagg tcacgggccg
    12061 tcgagccggc cggcgccagc accacgcccg gcgacttcac ccggccggag accgagtagg
    12121 agcgcggacc cttggcgccg ttcttgccgt ggcgggcgaa ccattcggag ccgtttttca
    12181 ggatgagcgg cacccagtag agcgtctcga tgttgttgac gagggtgggc cttccgaaga
    12241 ggccgacctg cgcgacgtag ggcggacgat ggcgcggcag tccgcgcttc ccctcgatac
    12301 tctcgatcat ggcgctttct tcaccgcaga tgtaggcgcc cgcgccgcgg cggacgcgca
    12361 ccgcgcctgt cggcaggatc ttggcggcct cgagcgctgc gatctcgcgc ttcaggatgt
    12421 caatgacggc gggatactcg tcccgcatgt agatgtagac ctcctcggcc tcgactgccc
    12481 aggcggcgat ggcgagccct tcgaggaact gatgcggatt ggtctcgaga tagtggcggt
    12541 ccttgaaggt gccgggctcg ccctcgtcgc cgttgaccgc catcaggcgc gggcccttgg
    12601 cgctgcgcac gagcccccat ttgcggcccg tcgggaaccc ggcgccgccg aggcctctga
    12661 gcccgccatc ggaaagctgc agaatgacgt cgtcgacggt ccgccgcccc tcgaggcagg
    12721 cctcaagcag ctcgtagccg ccttcggcgc gataggcgtc aaggctcttg taggccggga
    12781 tcggcgcatc gtgcgtgtcg gtcacggcga cggccacgat gctctcgacc gttgcgcgat
    12841 ggaccgtcac gtgtccgacg tgggcagccg gcgcatggtg gcaggccccg aggcacggcg
    12901 cacgcacgac gcgcgtcccg ggcggcggaa cggccgatag cgcggcaagc aatgcgtcgc
    12961 cgccgcccag catgcacgag aggctgtcgc agacgcggat gaccgttggc gctggtaagg
    13021 cctcgtcgtc catgacgaca tcgaagtggg cgtagaaggt cgcgacttcg aatacctccg
    13081 ccatcgagat cttcaggagc tcggcgacgg cctgcaggca accggcgggg aggcagcctt
    13141 cggcgtcctg caccttgtgc aggtactcga tcagcagggc gcgctcgtat ggccctggtc
    13201 cgatcagccg gacgacgcca tcgagctcgc tctgtctgag gacacgaccc ttgggcgcga
    13261 gcggcgccga gcgacgaccg gcgccggggt gaacccggcg cccagaccca gctcggctcg
    13321 cggcacctgc tccctcaccg ctcatcatca attccctaaa tttcgcgccg gctcgagcga
    13381 gccgctcatc cgacaggccg caacgaacac gtcgctacct tgtgcgcgtg atcgtcgcct
    13441 cgcgatcgga aaccgcgcgg cttgcggcag ttctggcata ccatatacct gagagctcga
    13501 atctgtcgag acgacgcatc gcggcgcatg caggcgcgcc gcggcgtctc acgtctgcat
    13561 gccccacttg cggatcgttc gcgcctcgat gttcttgaag atcaggttct cgacgagcag
    13621 gccgatagtg atcacggtga acaagcctgc gaacacggct gggatgtcga gctggttctt
    13681 gctttcgtag atgaaccagc cgaggccgcc cttgcccgag gaggcaccga agacgagctc
    13741 tgccgcgatc agggtgcgcc atgcgaacgc ccagccgatc ttgaggccgg tcaggatcga
    13801 ggggaacgct gctggtatga ggatcttgaa gatgtagcga aagccgccga ggccgtagtt
    13861 gcgtccgacc atgcgcaacg tattggagac cgatttgaag cccgagtgcg tgttgagcgc
    13921 aaccgcccag agcaccgagt ggacgagcac gaagatgatc gacttgtcgc cgaggccgaa
    13981 ccagatgagc gccagcggca ggagcgcgat cgccggcagc gggttgaaca tcgaggtcag
    14041 cgtctcgagg aagtcgttga agaactgcgt gaagatcgcg agcgcggtga gcagcatggc
    14101 aaggccgagg ccccaggcat aacccttgag cagcaggcgc acggagttga gcgccttcgg
    14161 catcagctcg cctgagaaga tcgccccgag caggccttcg agcccggaga cggggcttga
    14221 ccaagagaag gcgctcaagg ctgcggtgac ggcgccgggg gccggcttca tcgcggccca
    14281 gaacgcctgc agcgtaacgg tgaactgggg taccaggagc tcgttggtct ggcactggcc
    14341 ggtgaaagct gtcgacacgg cgccgtagag catcttcggc agcgagatga tgacatcgcc
    14401 gacccagacg atgttgcggg cgatctccag gagaggccag gcgagccaaa gcagcggtcg
    14461 cggccaacct tcgccgccta aggccgggat gatgtcgccg attgtcggcg tgaacttgaa
    14521 ggcgttcatg caactcgaat agccttgcca ggcaagcgcg aggatcagca ggatgagcgc
    14581 cttgcgcacc cagccgaggt tatagacgcg ctcaaacggg gtcagcggct tctcgacgac
    14641 gccgaagtcc tgcgtcgcct ccgtctccag gatgtactcg ggccgcgggg gcgtggtggc
    14701 tcggatggcg ctgtcagaca tgggcgacgg tcccctgctc ctcgacgcga tcggaaaaca
    14761 gcatgtggtg gatgcggtcg gagagcttct ggccggacgc cgggtcgacc ggatcggagc
    14821 cgtcactgtt gagctcggcc ttcacctggc ccggatgcgg cgacatcagg aggatacggt
    14881 tgccgatcag cacggcctcg gggatcgagt gcgtgacgaa tagaacggtg aacctggtat
    14941 cctcccagag ctgcaggagc tcgtcctgca tcttccgacg tgtgagggca tcgagggcgg
    15001 caaacggctc gtccatcagc aggatgtccg gctccatggc catggcgcgg gcgatggcga
    15061 cgcgctgctt catgccgccc gacagagtgt gcggataggc gttcgcgaac ttcgcgagat
    15121 tgaccttctc gatgtagcgc agcgcctgct cctcggccgc cttgccgcgc atgcggcctg
    15181 acgagacgag cgcgaacatg acgttctgct tcacggtttt ccagggcagc agctggtcga
    15241 actcctggaa caccatgcag cggtcggggc caggcttcct gatctgggcg cccttcagcg
    15301 tcatcgtgcc ctcagtcggc gccatgtagc cgccgacggc cttgaggagg gtggatttcc
    15361 cgcatcccga tggtccgagc agtacgaagc ggtcggattg cagcacatcg aagctcacgc
    15421 gatacgtcgc ggtcacgagg tgatgctttg tcttgtactg aagggtgacg ccgcgaacgc
    15481 cgagcagtgg ctgcggcgcg gcaaccggtt ccatcacagg ttttcctcca ccctgtccgt
    15541 cgcttgttgc gcccggcttg tccagcgcac cgggttccgg ccgaacgttg gcctatcgtg
    15601 cgagcaagcg caatacgtag ctgcggcatt ggtttgcccg gggtgcctcg atgccatcct
    15661 cggacagggc gctcagttga tgaagacgtc gatccgctgg cgtcgccggc cgagccaggc
    15721 gaggccgagg ccgagggcgc cgagcacgag ccaggccggc agtctcagca cgcgctgcac
    15781 accctgctcc cagacgatgg gcgggatgcg tcgttgaacg gtctcgacgg ccttgggcgc
    15841 aatcgtggtc gtgtgctcga gtagcgaggt gacggtgatg cgtccaggtc ccgcgatcgt
    15901 gcgcgtcccg tcgtagacga gcgcgatcac ggcgatcagc agcagcggcc ggctgatgaa
    15961 gcgcaacgcg aagagcgcac cggcccgccc ggtgcgcgtc agaacgacga acgcgacagc
    16021 caccgcgacg gccagaaacg gccaatatgt gaggaggaat gtcgtcgcct tgtccgtcat
    16081 gtcccaggtc cggttcgtcc gtcggcaaat tttagaccag gcgacaacac cgacctcaag
    16141 ccgtccgggt cgacgtggtc tggtccggtg tcaatacgtg agcgcggcct tgatggcagc
    16201 aagctcggct ttgggatcgg catagctctc gtagaattgg ctggcgcgcc ggtaccagta
    16261 gcggctgttc gcggcatcgc cctcgatctt atgcaggcag gcatgtatcc agcatcccgt
    16321 gctgtcgtcc tcgtagcgct gaactaccag atgcgcggcc tcccagtcgc cggcaatggc
    16381 gaggtccagc gccctgatca gctccgcgcg gacgtccatt cgtagaaccc tttcgctctg
    16441 aggctgcatg cggggcaact gccgcagcct tggccccaat catgcttcct gccgcgctcg
    16501 ccgagatagc acgtgtgcgt atccgacacg atcagatcga ccaaatcacg gccgccgagg
    16561 tcctcggcaa gtgcccatgt ggcagccttc gtgagccaca tcagtggtgt cgcaatctgg
    16621 acgcgccgat ccatgccgag cgagagcgcc gtctcgagcg cacgcatggt g
//
LOCUS       DAORNH010000069        16232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2730_length_16232_cov_2.635037, whole genome shotgun sequence.
ACCESSION   DAORNH010000069 DAORNH010000000
VERSION     DAORNH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16232
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2730_length_16232_cov_2.635037"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    285..450
                     /estimated_length=166
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<451..718)
                     /locus_tag="PK264_09200"
     CDS             complement(<451..718)
                     /locus_tag="PK264_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76102.1"
                     /translation="MPKHVRACGNQSDRTAEATASKAAAGKASLLRLALSYAKACIAA
                     FLVLLVLHSGLVLSGQPVNVSPARWLVAVCFAWAFGIPLLPVVMT"
     gene            939..1856
                     /locus_tag="PK264_09205"
     CDS             939..1856
                     /locus_tag="PK264_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD76103.1"
                     /translation="MSIYLPIAEIASDVLILTGLGLIVGFLSGMFGIGGGFIMTPLLI
                     ASGVPSAVSVSTGAASVMASGVSGAIAQWKRRNVDVRLGIILLLGGGIGAFIGVRCLT
                     LLRRFGQLDFAVAVLFSVMLGVVGTLMLVESLNTMWKARSGRPVSMKRAGQHAWFERL
                     PLKTRFPTSRIYMSALPPLLLGAFVGLLTGIMGVGGGFITVPALVYLFRAPTKVAVGT
                     SLFQVVFVAGFTTLLHAGVNQTIDVVLAIPIMLGGVVGAQYGARAGARLNAQQLRLLL
                     AIIVLIAAVRMVYGLTVMPVELFAVDAIR"
     gene            1898..2227
                     /locus_tag="PK264_09210"
     CDS             1898..2227
                     /locus_tag="PK264_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76104.1"
                     /translation="MRKSIVIVGFVMAAGSQAGAAWAQGKLADFDGSWTVRVEASSQC
                     PERLGPFGITIRAGSVSAPGGAGSVGINGAISFTASGYSFSGRLTGRSGSGSISGRCH
                     GNWSASR"
     gene            2255..3031
                     /gene="pgeF"
                     /locus_tag="PK264_09215"
     CDS             2255..3031
                     /gene="pgeF"
                     /locus_tag="PK264_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRD76105.1"
                     /translation="MTTAIEADCLSDARKIAHGFFTRHGGVSTGLYASLNCGLGSKDE
                     RAKVLENRGRVAEAFGASGSQLLTCHQWHSADAIVVTDPWAPDALPKADAMVTRTPGI
                     VLGALAADCAPVLFADPVAGVVGAAHAGWKGALGGVLEATIAAMESIGARRSRIRAAL
                     GPCIGADAYEVGPEFEATFIAKEKGHQRFFRRARPDARPYFDLPGFVLDRLGDAGLDT
                     VESAHHCTYEHDHDFFSYRRTTHRKEPDYGRQISAIMLRM"
     gene            3044..3442
                     /locus_tag="PK264_09220"
     CDS             3044..3442
                     /locus_tag="PK264_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD76106.1"
                     /translation="MKLLDHVSIAVADIDAARPFYDAAMAALGCSKVYDRADALGYGE
                     RCRPDLADETYLAVYAAHGSVADDRRHWCFKAASREAVRAFHAAALVNGGCSDGAPGL
                     RPHYHSAYYAAFVRDPSGNRLEAVCHRLEE"
     gene            3446..4018
                     /locus_tag="PK264_09225"
     CDS             3446..4018
                     /locus_tag="PK264_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD76107.1"
                     /translation="MALDRRRTIETFGDVAIDVTREGGGPVIVLLPSKARDSLDFDAA
                     AEGLAERGFRVIRPQPRGALGSRGPLTGLKLHNLARDVAEVIRAEGGEPAIVVGHAFG
                     NWVARTLAVMHPELVRGIVLAAAAARSVPAEITATVAAAANTGLDREKRLAALRLGFF
                     APGNDPSVARRLARRRQPLPDGRDRGDTAH"
     gene            4312..5022
                     /locus_tag="PK264_09230"
     CDS             4312..5022
                     /locus_tag="PK264_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD76108.1"
                     /translation="MSLDVVTLRDFYERPLGKLVRRELVQRIRAHWRRAHGTTLVGIG
                     YPTPYLGSFRGEARALAAFMPASQGAIIWPNESSVLTALIEDDRLPLADNSVDLMLAV
                     HCLELSDSLRLLLREMWRVLKPEGRLLAIVPNRRGVWARLERTPFGHGRPYSASQLDR
                     LLRDALFEPVSTDSALHMPPFKRPQLLGVARTIGRMSARIGPTFAGVILVEVRKEVSA
                     PTGRAIRETAAELATAKG"
     gene            complement(5026..6081)
                     /locus_tag="PK264_09235"
     CDS             complement(5026..6081)
                     /locus_tag="PK264_09235"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HRD76109.1"
                     /translation="MTDIAASAPHRPARRIPARELLSAAQLAEVRERVPWRGIGMIAH
                     AWAVILGAIALVAIWPNPLTVLLAIALIGSRQLGLAILMHDGAHGAFVKDERWNLALS
                     QWLCAYPIFAETRAYRRYHLQHHAHTQTEDDPDLVLSAPFPVTPASYRRKLLRDITGQ
                     TGFQQRKAQVLNALGPTSMPVAERLRRFASHLGPQIAVNAVLFAGMTAAGIWWAYPVL
                     WLLPLLTWFQVVVRVRNIAEHAVVGDSNDPLRNTRTTIASPLERAFLAPYYVNYHLEH
                     HLFFYVPAYNLPKVHRWLMAGPHRERLEVEKGYVRVMRKATALDSTLDKPGELVSSVR
                     RRQAGATVDRDQAAAGF"
     gene            complement(6081..6608)
                     /locus_tag="PK264_09240"
     CDS             complement(6081..6608)
                     /locus_tag="PK264_09240"
                     /inference="COORDINATES: protein motif:HMM:NF022214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HRD76110.1"
                     /translation="MAKARRQKSSSAPALASADEPLPLKPLAGWLRVVAAGLVIAPLW
                     IGYVTWSTFGPMLSGGVAAALGAVGDNPSLGPAIKWLLVTELLANTGLMLLALWALTL
                     MLGRHRWFPRVVIGLAVLSLTIVIGDSLIAHRLGLAHDGLAVWDILRNAVTALILVPY
                     MLISKRVRATFVEQA"
     gene            complement(6608..>7052)
                     /locus_tag="PK264_09245"
     CDS             complement(6608..>7052)
                     /locus_tag="PK264_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76111.1"
                     /translation="AETITYFEEALARPPGRSDDPLRDGVKMGTTLVGTPETISRGIN
                     KLIELSKGGIGGVMFRAHEWANREQTLRSYELFARWVMPRFQGALEMQTRSHAWVSAN
                     QKAILGENAAAFKKAYTDHGRDVPTDFDVRILGGALKEQKPGGKG"
     assembly_gap    7053..7204
                     /estimated_length=152
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7205..>7615)
                     /locus_tag="PK264_09250"
     CDS             complement(<7205..>7615)
                     /locus_tag="PK264_09250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD76112.1"
                     /translation="AAERTRNIRLGSGVTSLPYHHPYMVAQRFVQLDHMTRGRAMLGC
                     GPGALITDAYMLGIDPKTQRTRMDQSLDAITRLLKMEEPVSIKTDWFEMREARLHLAP
                     YTDPHFKIAVASTQTPAAMIAAGKYGLSVLSLGAG"
     assembly_gap    7616..7642
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8007..>8824
                     /gene="trpA"
                     /locus_tag="PK264_09255"
     CDS             8007..>8824
                     /gene="trpA"
                     /locus_tag="PK264_09255"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRD76113.1"
                     /translation="MAQATRIDRRFKELKAEGRAALVTFVMAGDPDFETAKRIITGLP
                     SAGADVIEIGMPFSDPMADGPAIQAAGVRALKGGQTLAKTLELVRAFRAKDKATPIVL
                     MGYYNPIYVYPAERFLDEAATAGVDGLIVVDLPPEADDELCLPAMKRGLNFIRLATPT
                     TDRKRLPAVLNNTSGFLYYVSITGITGAAAPDPGKVRAEVADIRRATNLPICVGFGVR
                     TPEQARAIAAGADGVVVGSALVNAIAGSLDGAGKATGKTAAAVLDLVSNLAKGVR"
     assembly_gap    8825..8834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9007..10065
                     /gene="accD"
                     /locus_tag="PK264_09260"
     CDS             9007..10065
                     /gene="accD"
                     /locus_tag="PK264_09260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRD76114.1"
                     /translation="MNWINNFVRPKIQSLLTTRREPPDNFWHKCGGCGQMSDRKALEE
                     SQFVFACCGHHGRIAANDRLKALFDKGEFKRIDIPKVPQDPLKFRDGRRYTDRIKDAK
                     AATGLEDAVLIGEGTVDERQVVAAAQDFRFLGGSLGMAAGEAVVTAMLAAVERKAPFI
                     LFAASGGARMQEGILSLMQMPRTTIAVQKLREAHLPYIVVLTDPTTGGVTASYAMLGD
                     VHIAEPGALICFAGPRVIQQTIRERLPEGFQRAEYLLEHGMIDMVVHRHKLRETLSRL
                     CGLFAGGAEPAKVAAGHKRDYKNGHAVDPKLIEAAGRTAGTPIEAEPVDGTERFDTAR
                     VSKAKKAADTTRKPAARE"
     gene            complement(10082..10552)
                     /gene="rnhA"
                     /locus_tag="PK264_09265"
     CDS             complement(10082..10552)
                     /gene="rnhA"
                     /locus_tag="PK264_09265"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRD76115.1"
                     /translation="MASADPVVIHTDGACSGNPGPGGWGAILVWGGHRREINGGEAET
                     TNNRMELLAAISALEALKRAVKVELHTDSAYLKDGITKWIHGWKRNGWKTADKREVKN
                     VDLWQRLDELVRKHEIDWHWVKGHAGHPENERADELARMGMAPFVVGRKKKSGS"
     gene            complement(10552..11532)
                     /locus_tag="PK264_09270"
     CDS             complement(10552..11532)
                     /locus_tag="PK264_09270"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HRD76116.1"
                     /translation="MAVYTEVSDDDMAAFVASYGLGRLHSYKGIAEGVENTNYLVVTD
                     RGNFILTVYEKRVNVEDLPFFLGLMEHLGHRGLTCPSPVRNAEGKALGELAGKPAAIV
                     TFLEGVWIRRPRVEHCAAVGAALARMHLAGQDFALRRENALGWRGWRPLFDRFAPRAD
                     EVSKGLGSLIASELDHLEASWPNSLPAGVIHADLFPDNVFFLNDELSGLIDFYFACND
                     ALAYDVAVCLNAWCFEPDHAFNVTKGRALLRGYLGVRALLPDERAALPMLCRGAALRF
                     LLTRAFDWINTPAGALVNRKDPMEYVRKLRFHQGLKHAGELGLDLADGAG"
     gene            complement(11536..12504)
                     /gene="ispH"
                     /locus_tag="PK264_09275"
                     /gene_synonym="lytB"
     CDS             complement(11536..12504)
                     /gene="ispH"
                     /locus_tag="PK264_09275"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRD76117.1"
                     /translation="MTTAKPALTILLAAPRGFCAGVDRAIQIVELALKKYGAPVYVRH
                     EIVHNRFVVESLKAKGAIFVEELDEVPDTGAPVIFSAHGVPKSVPEAARARNFFYLDA
                     TCPLVSKVHVEAARHHAEGHEIILIGHAGHPEVIGTMGQLPAGVVRLVETVADARTFT
                     PKSPSKLSYVTQTTLSVDDTAEIVSVLRERFPAIVGPHKEDICYATTNRQAAVKAVAP
                     RAERVIVVGAPNSSNSLRLVEVAERSGSPKALLVQRASEIPWAEFQGLDTIAITAGAS
                     APEILVDEIISAFRERFSVTLESVTTAEERIAFNVPRELRDEKAGA"
     gene            12769..13389
                     /locus_tag="PK264_09280"
     CDS             12769..13389
                     /locus_tag="PK264_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76118.1"
                     /translation="MNARIAISTVAALALTGCGMSSLTSGIGGGMFGSSAKSDTKSVT
                     TEGLLSAAKADAAGTVSAGADVAHGCPRVTVGSREGHLTIYEAGRAGDGLAVMHRGEI
                     MRTARECAIERGRVTVKYGFSGRVLLGPRGRPGPVALPVSVLVTDAKRERVAADKARV
                     DVTITSEKPEGYFSIVRTVTFNVPEGTRPGEFEVFVGFDQNVPGAG"
     gene            complement(13397..14281)
                     /gene="pdxY"
                     /locus_tag="PK264_09285"
     CDS             complement(13397..14281)
                     /gene="pdxY"
                     /locus_tag="PK264_09285"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HRD76119.1"
                     /translation="MARVLAISSQVARGHVGLSAIVPTLQRLGHEVTALPTILLSNHP
                     GHARFAGERVAAALLDRMIAALAPEGSASLAGEVDAILSGYLPSPEHVAVVEGAVLAA
                     RRERPSLPYLCDPVTGDDPKGLYVPQDVAEAIRDRLLPLATIATPNVFELAWMTGRDV
                     GDLGAIAVAASHLAPATVLVTSARDPAAAEDKLSNLLCVKAIEGERQDAVYRADVRRR
                     TGVPNGTGDMASALLLGHLLAGRAMSEALACTVGSLETVIGASLGARELALVASATAW
                     ADAPPAHVQRIRGGLWGG"
     gene            complement(14290..15456)
                     /locus_tag="PK264_09290"
                     /pseudo
     CDS             complement(14290..15456)
                     /locus_tag="PK264_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314982.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            15743..>16232
                     /locus_tag="PK264_09295"
     CDS             15743..>16232
                     /locus_tag="PK264_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRD76120.1"
                     /translation="MRRNRKAPWTRRLVAEHRLSLDDLVWPIFLIDGADRRVPVAHMP
                     GCDRVNVEEAVREAERAAKLGIPAIAPFPYVEKGLRDPDGREATKVQNLMGRAVAAIK
                     KAVPELGVITDVALDPYTSHGHDGLMRTDGSIDNDGTLEVLCRQALGLMAGLWWDIGD
                     GAG"
BASE COUNT         2623 a         5161 c         5421 g         2672 t
ORIGIN      
        1 gcgccccagt agcggctcgc atcgacctcg atcgggccga aggtgtcggt ctcggtgcgc
       61 gtcttggcgg cggtcatggg aggttcctcc gacgggaatg cagcgacttt ccgacccgat
      121 agcacgcggg tcggcgaggc tccactacac gctggtcgca ttgcccagaa tgcgcctacc
      181 ggccaagcct gctggcaatc cacaagccaa gtttcatgcc gcgttcacct gcctgcagct
      241 ttggcttcac gctcggaggg gcaccaggtc ttccctccgc ccgcnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgtcatcac aaccggaagc aatggaatgc
      481 cgaaggccca ggcgaagcag accgcaacca accagcgagc aggcgacacg ttgaccggct
      541 ggccagacag tacaagccca gagtggagca ccaacaggac gagaaatgcg gcgatacatg
      601 ctttggcgta tgacaaggcg aggcgcaaaa ggctcgcttt gccagccgcg gccttggaag
      661 ccgttgcttc cgccgtcctg tcggattgat tgccgcaggc ccggacgtgc ttcggcatgg
      721 gagttctgac gacccgtctg ccgaagccta ccggcatggt atccccgact tgcaagcgcg
      781 cgtggtcgtg gcaagcgctg gggtcgggca ttgttgcatc ctgagtttcg acgagcgcgc
      841 gacgaccgta gcaccggtga gttaacgcgc cgtttggtcc ggggattgtc ggctcgagaa
      901 tgcttgccgg ctcgaaatgt gcagactatt gcacggagat gagcatctat ctgccgatcg
      961 ccgagatcgc ctctgatgtc ctgatcctga cggggctcgg gctcatcgtc ggatttctgt
     1021 ccggcatgtt cggcatcggc ggtggcttca tcatgacgcc gttgctgatc gcgagcggcg
     1081 tgccgtcggc cgtatctgtg agcacgggcg ccgcctccgt gatggcctcg ggcgtctcgg
     1141 gagccatcgc acagtggaag cgccggaacg tcgatgtgcg actcggcata attctgctgc
     1201 tcggcggcgg catcggcgcc ttcattggcg tccgttgcct gacgctgctg cgccggttcg
     1261 gccagctaga ttttgcggtc gccgttctgt tcagcgtgat gctcggcgtg gtcggcacgc
     1321 tcatgctggt cgaaagcctc aacaccatgt ggaaggcgcg cagtggccgg cctgtctcaa
     1381 tgaagcgggc cggccagcac gcctggttcg aaaggctgcc gctgaagacg cgttttccga
     1441 cgagccgaat ctacatgagc gcgctgccgc cgctgctgct cggcgctttc gtcggcctgc
     1501 tgactggcat catgggcgtg ggcgggggct tcatcaccgt gccggcgctc gtctatctct
     1561 tccgcgcgcc gaccaaggtc gcggtcggga cgtcgctgtt tcaggtcgtg ttcgtcgccg
     1621 gctttacgac gctcctgcat gccggggtca accagacgat cgacgtcgtg ctggcgatcc
     1681 cgatcatgtt gggcggcgtc gtcggcgcac agtacggcgc ccgcgcgggg gcacggctca
     1741 acgcgcaaca gctccggttg ctgctggcga tcatcgtgct gatcgcggcc gtccgcatgg
     1801 tctacgggct cacagtcatg ccggtcgagc tctttgccgt cgacgccatc aggtagctcc
     1861 ggctatcctg acgcgcgggc acaacacggg tgaagtcgtg cgcaagtcga tcgtcatcgt
     1921 tggtttcgtg atggctgccg gctcgcaagc gggcgctgcc tgggcccaag gcaagctcgc
     1981 cgatttcgac ggcagctgga cggtgcgcgt cgaggcgagc tcgcaatgcc ccgaacggct
     2041 cgggccattc gggatcacga tccgcgcggg ctccgtgagc gctccgggag gcgccggcag
     2101 cgtcggcatc aatggcgcga tctcgttcac tgcgtcgggc tattcgttca gcggccggct
     2161 cacagggcgc agcggatcgg gcagcatctc cggccggtgt catggtaact ggagcgccag
     2221 tcgctgacct tctgaccgcc ggagcagatc gcgcatgacc acggcaatcg aggcggactg
     2281 cctctctgac gcgcgcaaga tcgcgcacgg cttcttcacg cggcacggcg gtgtctcgac
     2341 aggactctac gcgagcctca actgcggcct cggctccaag gacgagcgcg caaaggtgct
     2401 cgaaaaccgt ggccgcgttg ccgaggcatt cggcgcgagt ggctcgcagc tcctcacctg
     2461 ccatcaatgg cacagcgccg atgcgatcgt cgtcaccgat ccttgggcgc cggacgcgtt
     2521 gcccaaggcc gacgcgatgg tgacgcggac gcccggcatc gtactcggcg cgctcgcggc
     2581 cgattgtgcg ccggtcctct tcgccgatcc ggtcgcgggc gtggtcggcg ctgcccatgc
     2641 cggctggaaa ggcgcactcg gaggcgttct cgaggcgacg atcgcggcaa tggagagcat
     2701 cggcgcgcgg cggagccgga tccgagccgc cctcggcccc tgcatcggcg ccgacgccta
     2761 cgaggtcggt cccgagttcg aggcgacgtt cattgccaag gagaaggggc accagcgctt
     2821 ctttcgtcgc gcgcggccgg acgcgcgacc ctacttcgac cttccgggct ttgtgctcga
     2881 ccgtctcggc gatgccgggc tcgatacggt cgagagcgca catcattgca cctacgagca
     2941 cgatcacgat ttcttctcct accgacggac gacgcatcgc aaggagccgg attacggccg
     3001 gcagatttcg gcgatcatgc tcaggatgtg agagttcacc gacatgaaac ttctggacca
     3061 tgtctcgatc gcggtcgccg acatcgatgc ggcgcggccc ttctatgatg ccgcgatggc
     3121 ggcgcttggc tgcagcaagg tttacgatcg tgctgacgcg ctcggctatg gcgaacgctg
     3181 ccggcccgat ctggcggacg agacgtatct ggccgtctat gcagcacacg gctcggtcgc
     3241 tgatgacagg cgccactggt gcttcaaggc agcctcgcgc gaggccgtgc gcgcgttcca
     3301 tgccgccgcc ctcgtgaacg gcggctgttc ggatggcgca ccagggcttc ggccgcacta
     3361 tcattcggcc tactatgcgg catttgtgcg cgacccgtcg ggcaaccggc tcgaggccgt
     3421 ctgccatcgg ctggaggagt gagcgatggc ccttgatcgg cgacgcacga tcgagacttt
     3481 tggcgatgtt gcgatcgatg taacccgtga gggtggcggg ccggtcatcg tccttctacc
     3541 ctcgaaagca cgcgattccc tcgatttcga tgccgcggcc gagggactgg ccgagcgcgg
     3601 atttcgcgtg atccggccac agccacgggg agcgctcggc agccgggggc cgctcacggg
     3661 gctcaagctg cataatctcg cccgtgacgt tgccgaggtc attcgtgccg aaggcggtga
     3721 gcccgcgatc gtcgtcggcc acgcattcgg gaattgggtg gcgcgcacac tggccgtgat
     3781 gcaccccgag cttgtgcgtg gcatcgtcct cgcagcggcg gcggcgcgga gcgtgccggc
     3841 cgagatcacg gcaacggtcg ctgcagcagc gaacacgggt ttggatcgtg agaagcggct
     3901 cgcggcgctc cgcctcggct tcttcgcgcc cggcaacgat ccgtcggtgg ctcgacggct
     3961 ggcacgcaga cgccaaccgt tgccagacgg ccgcgaccgc ggcgacaccg cgcactgagt
     4021 tctggcatgc ggggacggcg cccattctcg agatcattcc ggaacacgat cccttcaagc
     4081 cgcgcgcgag ctggggcgag gcccgggacg agctcggtga ccgcgtaatg atcgtgacgg
     4141 tgccagacgc gagccacgcg ctcttcccgg agaggccggg cgcggtggtc gaggcgattg
     4201 caggttgggc gaagcaatcc acctgagtgt gcgacgctga aagtgcgacg gaattggttc
     4261 tcagggagct ttgcggcata ttgcgagaag cccctctctc aatggaaccg catgagcctc
     4321 gatgtcgtca cgctccggga cttctacgag cgcccgctcg gcaagctcgt caggcgtgag
     4381 cttgtgcagc ggatccgggc gcactggcgg cgcgcgcacg ggacgacgct ggtcggcatc
     4441 ggctatccga cgccctatct cggctcgttc cgtggcgagg cacgggcgct ggcggcgttc
     4501 atgccggcat cccagggagc gatcatctgg cccaatgaga gctccgtgct gacggcgctc
     4561 atcgaggacg accgcttgcc gctcgccgac aactctgtcg atctgatgct cgccgtgcac
     4621 tgcctcgagc tcagcgattc gctccgcctg ctgctccgcg agatgtggcg cgtcctgaag
     4681 cccgaagggc ggctgctcgc catcgtgccg aaccggcgcg gagtttgggc gcggctcgag
     4741 cggaccccgt tcgggcatgg ccggccctac agcgcgagcc agctagaccg gttgctgcgg
     4801 gacgcgctgt tcgagccggt ctcgacggat tcggcgctcc acatgccgcc attcaagcgc
     4861 ccgcagttgc tcggcgtcgc gcgcaccatc ggccgcatga gcgctcgcat cgggccgact
     4921 ttcgccggcg tgatcctggt cgaagtccgc aaggaggtct cggctccgac cggccgagcg
     4981 atcagggaga cggccgcgga gcttgcgacc gccaagggct gagcctcaga accccgccgc
     5041 ggcctgatcg cgatcgacgg tggccccggc ctgccggcgc cgaacggagc tcacgagctc
     5101 gcccggcttg tcgagggtcg aatcgagcgc agtcgccttg cgcatcacgc ggacgtaacc
     5161 tttctcgacc tccaagcgct cgcgatgcgg ccccgccatc agccagcgat gcaccttcgg
     5221 caggttgtag gccgggacgt agaagaacag gtgatgctcg aggtggtagt tgacgtagta
     5281 gggtgcgagg aaggcgcgct cgagcgggct cgcgatcgtc gtccgcgtat tgcgcagcgg
     5341 gtcgttgctg tcgccgacca cggcatgttc ggcgatgttg cgcacgcgca cgaccacctg
     5401 gaaccaggtg agcagcggca acagccagag gaccggataa gcccaccaga tgccggcggc
     5461 cgtcataccg gcgaacagca cggcattgac tgcgatctgc gggccgaggt ggctggcgaa
     5521 acgccggaga cgctcagcca ccggcatcga tgtgggcccg agcgcgttca ggacctgcgc
     5581 cttgcgctgc tgaaacccgg tctgcccggt gatgtcgcgg aggagcttcc gacgatagct
     5641 cgccggcgtc accggaaatg gtgcagatag cacgaggtcc gggtcatcct cggtctgcgt
     5701 gtgtgcgtga tgctggaggt ggtagcggcg ataggcgcgc gtttcggcga agatcgggta
     5761 ggcacacagc cactggctca aggcgaggtt ccaccgctcg tccttcacaa aagcgccgtg
     5821 cgcgccgtca tgcatgagga tggcgaggcc gagctggcgc gagccgatca gcgcgatggc
     5881 gagaagaacg gtgagcgggt tcggccagat ggcgacgagg gcgatcgcgc caaggatcac
     5941 ggcccaggca tgcgcgatca tgccgatccc cctccagggc acgcgctcgc gtacctcggc
     6001 gagttgcgcc gcggacagca gctcgcgcgc gggaattcgg cgggcggggc gatggggtgc
     6061 cgatgctgcg atgtcggtca ttacgcttgc tcgacgaacg ttgcccggac gcgctttgat
     6121 atcagcatgt acggaacaag aatgagcgcg gtcaccgcat ttcgcaagat gtcccatact
     6181 gcgagcccgt catgggccaa tccaaggcga tgggcaataa ggctgtcgcc gatcacgatc
     6241 gtcaggctga gcacggcaag cccgatcacg acgcgtggaa accagcggtg gcgcccgagc
     6301 atcagcgtca acgcccagag cgccagcagc atgagccccg tgttggcgag gagctcggtg
     6361 acgagcagcc atttgatggc cggaccgagg ctcgggttgt cgccgaccgc gccgagcgca
     6421 gccgcgaccc cgcccgagag catcggcccg aatgtggacc atgtgacgta gccgatccag
     6481 agcggcgcga tgactaggcc ggccgcgacg acgcgcagcc agcctgcaag cggcttcagt
     6541 ggcagcggct cgtcggcgga cgcaagcgct ggtgcgctcg agctcttctg ccgccgcgcc
     6601 ttcgccatca gcccttgccg cccggcttct gctccttcag cgcgccgccg aggatgcgca
     6661 cgtcgaagtc ggtcggcacg tcgcggccgt gatcggtgta ggccttcttg aaagcggcgg
     6721 cgttctcgcc gaggatcgcc ttctggttcg cgctgaccca ggcgtgcgat ctcgtctgca
     6781 tctcgagcgc gccctggaaa cgcggcatca cccagcgggc gaacagctcg taggagcgca
     6841 gcgtctgctc tcggttcgcc cactcgtgcg cgcggaacat cacgccgccg atgccgccct
     6901 tcgaaagctc gatcagcttg ttgattccgc gcgagatagt ctcgggcgtg ccgacgaggg
     6961 tcgtgcccat cttgacgccg tcgcgcagcg gatcgtccga acggccgggc gggcgggcca
     7021 gcgcctcctc gaaataggtg atggtctcgg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnccggcg ccgagcgaga gcaccgaaag gccgtacttg ccggcggcga tcatcgccgc
     7261 aggcgtctgc gtcgaggcga cggcgatctt gaagtgcgga tcggtgtagg gggccagatg
     7321 cagccgtgcc tcgcgcatct cgaaccagtc ggtcttgatc gagaccggct cttccatctt
     7381 cagaagccgc gtgatggcgt cgagcgactg gtccatgcgt gtgcgctgcg tcttcggatc
     7441 gatgccgagc atgtaggcat cggtgatgag cgcgcccgga ccgcagccga gcatggcgcg
     7501 gccgcgcgtc atgtgatcga gctgcacgaa gcgctgcgcc accatatagg ggtggtgata
     7561 gggcaggctg gtcacgcccg agccgaggcg tatgttgcgc gtgcgctcgg cggcgnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnccgagtga tgctcgccga tccaagcctc gtcatatccg
     7681 agcgtgtcga gccactggat gagctcgagg tcgcggccga aggcgagcgt tgggttgtcg
     7741 cccagccgat ggaacggagc gaggaagatg ccaaagatga gacctctgtg agccatggag
     7801 cctgatgcct tgcggtcggt tgatgatccg gatcgagcgg gagcctagcg gcgcgcggcg
     7861 cggtgcgcca gagcgcgaag cgcgaagccg ccctgcagtc gaccaatccg gtctcggatc
     7921 gccgcgagaa ccaacgccaa gcaaaatccc ttgacccatg cgcgcatgcg ggcctacccg
     7981 ttcggcgctg acggggaacc catacaatgg ctcaagccac tcgcatcgat cgccgcttca
     8041 aggaactgaa agcagagggc cgggccgcgc tcgtcacgtt cgtgatggcg ggtgatcccg
     8101 atttcgagac agcgaagcgc attatcaccg ggctaccatc tgccggcgcc gatgtgatcg
     8161 agatcggcat gcccttttcc gatccgatgg cggacgggcc ggcgatccag gccgcaggcg
     8221 tgcgcgccct gaaaggcggc cagacgctcg ccaagacgct cgagctggtg cgggcgttcc
     8281 gagcgaagga caaggcgacc ccgatcgtac tcatgggcta ctacaatccg atctacgtct
     8341 atccggccga gcgcttcctc gacgaggccg cgacggccgg cgtcgacggg ctgatcgtcg
     8401 tcgatctgcc gcccgaagcc gatgacgagc tctgcctgcc ggcgatgaag cgcgggctca
     8461 atttcatccg cctcgcgaca ccgacgacag acaggaagcg cctgccggcc gttctcaaca
     8521 acacctccgg cttcctctat tacgtgtcga tcaccggcat caccggggcc gcggcgcccg
     8581 atcccgggaa ggtccgcgct gaggtggccg acatccggcg ggcgacgaat ctgcccatct
     8641 gcgtcggttt cggcgtgcgc acacccgagc aggcgcgggc gatcgctgcg ggcgctgacg
     8701 gggtcgtggt cggctcggcg ctggttaacg ctatcgccgg ttccctcgac ggcgcgggca
     8761 aggccactgg aaagacggcc gccgccgtcc tagatttggt gagcaacctc gcgaagggcg
     8821 tgcgnnnnnn nnnncagccg ctgacaggcc tctgccactg ctccttcgct gctgcaccca
     8881 ggggcaattt gccgctgcgg tgtcatcatg cgccgggcta tgtgggtccg gtggctggtt
     8941 cggccacgtg tcgccgccgc ccctaaaacg cgcggaccac gagaggcctg gcgggagctc
     9001 atctacgtga actggatcaa caatttcgtc cggccgaaga ttcagagcct gctcacgacg
     9061 cgccgtgagc cgcccgacaa cttctggcac aagtgcggcg gctgcgggca gatgtcggac
     9121 cggaaggcgc tcgaggagag ccagttcgtc ttcgcatgct gtggccatca cgggcgcatt
     9181 gcggccaacg accgcctgaa ggcgctgttc gacaagggcg agttcaagcg catcgacatc
     9241 ccgaaggtgc cgcaggaccc gctcaagttc cgtgacgggc ggcgctacac tgaccgcatc
     9301 aaggacgcaa aggccgcgac cggcctcgag gacgcggtgc tgatcggtga aggcacggtc
     9361 gacgagcgcc aagtggtcgc cgcggcgcag gacttccgtt ttctcggcgg ctctctcggc
     9421 atggcagcgg gcgaagccgt ggtgacggcg atgctcgctg ccgtcgagcg caaggcgccg
     9481 ttcatcctgt tcgcggcctc gggcggtgca cgcatgcagg aaggcatcct gtcgctcatg
     9541 cagatgccgc gcacgacgat cgccgtgcag aagctgcgcg aggcgcatct cccctacatc
     9601 gtcgtgctga ccgacccgac cacgggcggc gtcacggcct cctacgccat gctcggcgac
     9661 gtccacatcg cggagccggg cgcgctcatc tgcttcgcgg gtccccgcgt catccagcag
     9721 acgatccgcg agcgactgcc tgagggcttc cagcgcgccg aatacctgct cgagcacggt
     9781 atgatcgaca tggtcgtgca tcgccacaag ctgcgcgaga ctctgtcacg gctttgcggg
     9841 ctgttcgcgg gtggcgccga gccggcgaag gtcgcggccg gccacaagcg cgactacaaa
     9901 aacggccacg cggtcgaccc gaagctgatc gaggcagcgg gccggaccgc cggcacgccg
     9961 atcgaggccg agccagttga cggcacggaa cggttcgaca cggcccgcgt cagcaaggcg
    10021 aagaaggctg cagacacgac acgcaagcct gctgcgcggg agtagcgccg gcggcgcgtt
    10081 gctacgatcc tgatttcttc tttcggccca ccacgaaagg cgccatcccc atccgcgcga
    10141 gctcgtcggc gcgttcgttc tcgggatggc cggcatggcc ttttacccag tgccaatcga
    10201 tttcgtgctt cctgacgagc tcatcgagcc gctgccagag gtcgacgttc tttacctcgc
    10261 gcttgtcggc cgttttccag ccattgcgct tccagccgtg aatccacttg gtgatgccgt
    10321 ctttcaggta ggcgctgtcg gtgtgcagct cgactttgac ggcgcgtttg agggcttcca
    10381 gcgccgaaat ggctgcgagc agctccatgc ggttgttggt ggtctcggct tcgccgccgt
    10441 tgatctcgcg ccgatgccca ccccacacga ggatcgcgcc ccagccccct ggcccaggat
    10501 tgccagagca ggcgccgtcg gtatggatga cgaccggatc agctgacgcc atcagccggc
    10561 cccgtcggcg agatcgaggc cgagctcgcc tgcatgcttc aggccctggt gaaagcggag
    10621 cttgcgcaca tactccatag ggtccttgcg attgacgagc gcgcccgcgg gtgtgttgat
    10681 ccagtcgaag gcgcgcgtca gcaggaagcg gagggctgca ccccggcaga gcatcggcag
    10741 cgccgcgcgc tcatccggta ggagagcgcg gacaccaaga tagccgcgca gtagcgctcg
    10801 ccccttggtg acgttgaagg cgtgatccgg ctcgaagcac caggcattga ggcatacggc
    10861 gacatcgtag gcgagggcgt cgttgcaggc gaaatagaag tcgatgaggc cggagagctc
    10921 gtcgttcagg aagaagacgt tgtccgggaa cagatcggca tggatgacgc cggcgggcag
    10981 cgaattcggc catgacgcct cgaggtgatc gagttcgctt gcgatgagcg atccgaggcc
    11041 cttggaaacc tcatcggcgc gcggcgcgaa ccggtcgaag agcggccgcc agccgcgcca
    11101 gccgagcgcg ttctcgcgac ggagcgcaaa gtcctggcca gcgagatgca tgcgggcgag
    11161 tgccgcgccg acggctgcac agtgctcgac gcgggggcgg cgaatccata cgccctcgag
    11221 gaaggtgacg atcgcggcag gcttccccgc gagctcgccg agtgccttgc cttcggcatt
    11281 gcgcacgggg ctcggacagg tgaggccacg gtggccgaga tgctccatga ggccgaggaa
    11341 aaacggcagg tcctcgacgt tcacgcgctt ctcgtagacg gtgaggatga agttgccgcg
    11401 gtccgtcacg acgagatagt tcgtgttctc gacaccctcg gcgatgccct tgtaggagtg
    11461 cagacggccg aggccgtagc tcgcgacgaa tgcggccata tcgtcgtcgg agacctccgt
    11521 gtagacggcc atgactcaag cccccgcctt ctcgtcgcgc agctcgcggg gcacattaaa
    11581 ggcaatgcgc tcctccgccg tcgtgaccga ttcgagcgtc accgagaagc gctcgcggaa
    11641 ggcggagatg atctcatcga cgaggatctc gggcgccgag gcgccggccg tgatcgcgat
    11701 cgtgtccagg ccttgaaact cagcccaggg gatctcggag gcgcgctgga cgagcagcgc
    11761 cttcggcgaa ccggagcgct cggcgacctc gacgagccgg agcgagttcg agctgttggg
    11821 cgcgccgacg acgatcactc gctcggcgcg cggcgccacc gccttgacgg ccgcctgccg
    11881 gttggtcgtc gcgtagcaga tgtcctcctt gtgggggccg acgattgcgg gaaagcgttc
    11941 gcgcagcact gagacgatct cggcggtatc gtccacggaa agcgtggtct gcgtcacgta
    12001 agagagcttt gacggcgatt tcggcgtgaa ggtgcgcgcg tccgcaaccg tctcgacaag
    12061 gcggaccacg cccgccggta gctgccccat cgtgccgatc acttccgggt gccccgcgtg
    12121 cccgatgaga atgatctcgt ggccctcggc gtggtgccgc gcggcttcca cgtgcacctt
    12181 cgacaccagc gggcaggtgg cgtcgagata gaagaagttg cgcgcgcggg cggcctccgg
    12241 aacggacttc ggcactccgt gtgccgaaaa tatcacaggc gctccagtat ctgggacctc
    12301 gtccaattcc tccacaaata tggcgccttt cgccttgagg ctctccacga cgaagcggtt
    12361 atgcacgatc tcgtgccgga catagacggg cgcgccgtac ttcttgagtg cgagctcgac
    12421 gatctggatg gcgcgatcta cgcccgcgca gaagccgcgc ggggcggcga gcaggatggt
    12481 tagggcaggc ttcgcggtcg tcatgttgtg cccgatgtcg tgcctgatcg aggttgccgg
    12541 caagctcggc cggtttgatg ttcggcaagg acgcgtattg ttctaacggt taaggacctt
    12601 caggcgctag cagttggtcc ccggatgcgg agagtgatac acttgagtcg gcaactgaca
    12661 aatagccacg cgaatcatgg cgggttgccg gcgtgcgcag ggggcttggt ggcagagatc
    12721 cgcaagagag atcgccatgg gcaaggggcg cgaacacagg ctcgatcgat gaacgctcgc
    12781 atcgcaatat cgaccgtcgc ggcgctcgcg ctcacaggct gtgggatgtc gtcgctgacc
    12841 tccgggatcg gcggcggcat gttcggctct agcgccaaga gcgatacgaa gtcggtgacc
    12901 actgaaggac tcctttcggc agccaaggca gacgcggcgg gcaccgtgag tgccggtgcc
    12961 gatgtcgctc atggctgccc gcgcgtcacc gtcggttcgc gcgaaggcca cctcacgatt
    13021 tacgaggcgg gtcgggccgg tgacggcctc gccgtcatgc accgcggcga gatcatgcgc
    13081 acggcgcgcg aatgcgcgat cgagcgtggc cgtgtcacgg tgaaatacgg cttctccggt
    13141 cgcgtgctgc ttggaccgcg tggacggccg ggacccgttg cgctgcctgt ctctgttctc
    13201 gtgacggatg ccaagcgcga gcgcgtcgcc gccgacaagg cccgcgtcga cgtcaccatc
    13261 acatcggaaa agcccgaggg ctatttttcg atcgtgcgca cggtgacgtt caatgtgccc
    13321 gaagggacgc ggccgggcga gttcgaggtc ttcgtcggct tcgaccagaa cgtgccgggc
    13381 gccggctgac acgatctcag ccgccccata gcccgcctct aatgcgctga acatgtgccg
    13441 ggggagcgtc tgcccaggcg gtggccgacg cgaccaaagc tagctcgcgc gcgccgaggc
    13501 tcgctccgat caccgtctcg aggctgccga cagtgcatgc aagcgcttcg ctcatcgccc
    13561 gaccggcaag caagtggccg agcagcagcg ccgatgccat gtcgcccgta ccgttcggta
    13621 cgcccgtgcg gcgccgcacg tccgcgcgat agacggcatc ctggcgctcg ccctcgatcg
    13681 ccttgacgca caacaagttt gagagcttat cttctgctgc cgccgggtcc ctcgccgaag
    13741 tcacgagcac ggtcgccggc gcaaggtggc ttgccgcgac cgcgatcgcg cccaagtcgc
    13801 caacatcgcg gccggtcatc cacgcgagtt cgaatacgtt cggcgttgca atcgtcgcca
    13861 gcggcagcag gcgatcgcga atggcctcgg cgacgtcctg gggcacatag aggcctttcg
    13921 gatcatcacc cgttacagga tcgcagaggt aggggaggct cggccgctcg cggcgcgcgg
    13981 ccagcaccgc cccctcgacg accgcaacgt gctcagggct cggcagatag ccgctgagaa
    14041 ttgcgtcgac ttcacccgcg aggctcgcgc tcccctccgg cgcgagcgcc gcgatcatgc
    14101 ggtcgaggag cgccgcggcg acgcgctcgc cggcaaagcg cgcgtggccc ggatggttcg
    14161 agagcaggat cgtcggtagc gcggtcacct cgtggcccaa gcgctgcaag gtcggcacga
    14221 ttgcggagag cccgacgtgg ccgcgggcaa cctgcgacga gatcgcgaga acgcgtgcca
    14281 tggtcggcct caatacacgt tgagttcacg cacggcggcg ccggtgatca cgcgctggaa
    14341 ccccagtcgc gtgaggtcga tcgagcagga gcggccatgc acgaccatct cggcgagcgc
    14401 gcggccgaca gctggagagt gctgcagtcc gtggcccgag aacccgcagg cgaacaggat
    14461 attccgcagc tcgtgatgtg gcccgagaat ggcgttctgg tcgagcaggt tgtagtcgta
    14521 gtggcaggac caggcattgg tgaccttgac ggcctcgaat gcgggcacgc gatgcgcgag
    14581 ccgcggccag acctctgcct cgaagaaggc gtggtcgatc tcgaagtcct cggtgtcggg
    14641 gtcggcatcc tccggcggct ggataccgga gaggaagtat tttccctccg gccggaagta
    14701 cagcccgctc gggtcgatca tgagtcccgc gccggtgagc ggttcacggc agtcgaccac
    14761 gaacacgagt cgcttcttcg gcctgacggg aagatcgatc cccgccatcg ccgcgacctt
    14821 tccggagtgc cagcccgcgg cgatgatcac ggtgccggcg ctgatctcgc caccggactt
    14881 gaggcgcacg cttcgcgcac tgccaccgcc cgtgccgatg ccgacgacct cgtcgtgcgc
    14941 gtacgtcacg cccttggcgc gggcgccatt gcggaacagc gtcatcaacg catgcggatc
    15001 tacccagcct tcgccggtgc ggccccagcc cgcggcggcg aggccttccg tcgacatcca
    15061 gggaaattgc cgcttcaacg cggcgggctc gagcagctcc gtatctgcac cgagccggtg
    15121 ctggagggcg atcgcctgct cgagattggc gcggccctcc ggcgaggcca taatgaggta
    15181 gccgcgctca cggaacgaga tgtcggcgtc cggcccgaac tcgcccttga tattcttgaa
    15241 gagagacagg ccgaactgcg agagatggat gttctcctcc gtcgtgaact actggcggat
    15301 cgaggcgacg gagcgtcccg tggcgcagaa ctggtagctc gggtccttct cgatgacggt
    15361 caccgagccg cgatagccct cgcgcctcag gaaataggcg atagagctgc cgaccaccgc
    15421 gccgccgatg atcacaacgt ctgccgaatt cgccatgcca tccccgctcg cgctcgattt
    15481 gcggtgatct ttgcccgctc gcaccactca tgtcatcgcc catcgacgtt ggcctcaggc
    15541 ctgcgctcaa tttcatgcgg tttcatccgc tgacaggcgc gcgcgcgtac tttagagtgg
    15601 tttcaaactc aacgtgtgcc ccgaggccga catgtcgaac aaacgagcag gcggattgcg
    15661 cttggatagc cccgtgaagc cggaggcgcg cgccgccgct cccccaccga cgggtccgtc
    15721 gggcgcgttt cccagcgttc gcatgcgtcg caaccgcaag gcgccctgga cgcggcggct
    15781 cgtcgccgag caccgcttga gcctcgacga tctcgtctgg ccgatcttcc tgatcgatgg
    15841 cgccgatcgg cgcgttccag tcgcgcacat gccgggttgc gaccgcgtca acgtcgagga
    15901 ggccgtgcgt gaggcggagc gggcggcgaa actcggcatc ccggccatcg cgccgttccc
    15961 ctacgtcgag aagggcctgc gcgacccgga cggccgcgag gcgaccaagg ttcagaatct
    16021 gatgggccgc gccgttgccg cgatcaagaa ggccgtcccc gagctcggtg tgatcaccga
    16081 cgtggcgctc gatccctata ccagtcacgg gcatgacggc ctgatgcgca ccgacggctc
    16141 gatcgacaat gacggcacgc tcgaggtgct ctgccggcag gcgctggggc tgatggcggg
    16201 gctctggtgg gacatcggcg acggggccgg gg
//
LOCUS       DAORNH010000070        16147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2757_length_16147_cov_2.798782, whole genome shotgun sequence.
ACCESSION   DAORNH010000070 DAORNH010000000
VERSION     DAORNH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16147
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2757_length_16147_cov_2.798782"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1201
                     /locus_tag="PK264_09300"
     CDS             47..1201
                     /locus_tag="PK264_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD76121.1"
                     /translation="MSAAIAFDQLRLPPECQTLRKEIRAFLAEEEAAGTFVRGSVEDL
                     YSPEFSRKVGARGWIGMTWPKKYGGRERSYLERFVVTEEFRVANAPVRRHFVADRQSG
                     PVILKYAPEHIKSDILPRICRGEVGFAIGMSEPGSGSDLFAAKTKATPVKGGFLVNGT
                     KVWTSMAHHADYMIGLFRTSQPTKENRRHGLTQFLVDMKVKGIKVNPIYQMTGLHDFN
                     EVVFEDVFIPEDRVLGEVDGAWKQATSELAYERSGPERFLETFYVLLSLVRAIGEKPD
                     LRAAEGIGRLVAQVHTLRRMSVSVAGMLAEGKEPVLEAAIVKDVGTIWEQRLPGRARE
                     LAAFIEDEVANRATFEEQLQFATLVAPKLTIQGGTTEILRGIIARGLGLR"
     gene            complement(1270..2277)
                     /locus_tag="PK264_09305"
     CDS             complement(1270..2277)
                     /locus_tag="PK264_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76122.1"
                     /translation="MTAWYKGVAAAGLLAATIAGAATTARAEVDEVHLVRQFGIGYLP
                     LTIMLHEKLIEKHAAKAGMPNLKTKWSVLGNAVPINDGLIAGTIHFGSGGVAPMVTAW
                     DRTRGSVRMHGVGALCALPVFLVSRHPYKSVKDFKENDRISMAGAGQSIQTLYLQIAV
                     AKEFGIANFKKLNPLFVNLSHPDGLAALLSGGEVSASFLAPPFQYRAIDKGMHKVLSS
                     YDVMGGPTSFLSVWSTGAFRDANPKTYKAVVDAFREATGIINADRARAAAIYVKDVSG
                     KEKIEDVIKSLSDPEFIYDLGPKKMMPLVDFMHSAGIIKSKPASWKEVFFPEVHDLKG
                     D"
     gene            complement(2319..>3300)
                     /locus_tag="PK264_09310"
     CDS             complement(2319..>3300)
                     /locus_tag="PK264_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD76123.1"
                     /translation="SQIFAAGPPVVERSLGQKISKEELGGPGLAVDQAGTIDNRATSE
                     AECFALIRRFLSYMPGNVWELPPAIAANDPAGRAEDELLRIVPKDRRRPYNMRRLVDL
                     VVDRGSLFEIQPTFGRALITALARLNGEVVGIVAHNPMVYGGAMDVRAARKQAHFVEL
                     CDCFHIPLVFFVDVPGLMVGRDAEMAATLREGMRAVYVGLQASVPMMSVVVRKCYGMA
                     GMGATDKNGLEFKVAWPSAEWGSLPIEGGVAAAFRREIAAARDPKARERELEAELRAK
                     ASPMRTAEAFGVEDIIDPRETRAYLCRFMAASRARRRTSLGPKPKYGVRP"
     assembly_gap    3301..3339
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3340..3928)
                     /locus_tag="PK264_09315"
     CDS             complement(<3340..3928)
                     /locus_tag="PK264_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD76124.1"
                     /translation="MNDQPKTPPAAEDWSAELEELAFRRAEAARLGGDAAVARHHEQG
                     RLTIRERIAGLADPGSFHEIGGLTGQGRYDGDRLVGVTPAPYVMGLAEIDGRPVAIGG
                     EDFTIRGGVSWSGDRKKGGQGGFVEDMAASYRIPLVNLIDGAGGSVASITRRGHAVFP
                     GVHGFERSVELMGKVPVVCAVLGTAAGGPAGRAILS"
     gene            complement(3953..4174)
                     /locus_tag="PK264_09320"
     CDS             complement(3953..4174)
                     /locus_tag="PK264_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRD76125.1"
                     /translation="MAKIEARSEVTGIIAKIEASAGDRLEAGSTVLFVESMKMQIPVV
                     VDDPATLKQVLVAVGDTVSEGQVVAVLES"
     gene            complement(4203..5792)
                     /locus_tag="PK264_09325"
     CDS             complement(4203..5792)
                     /locus_tag="PK264_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD76126.1"
                     /translation="MSETGKKPGKPPEAKAAVSHGERIASLGRRRTQALAMGGAEKLT
                     RRKAKAGVLDARERLDGLLDAGSFIESGLHGVSSSRPEDRDRSPADGKVAGFGRIEGR
                     EVAVVSNDFTVMGASSSSTNGRKIAHMKRVATTRGLPLVFLGESSGARMPDHMGARGM
                     ATLLGNDPTQYVRQRETPWASATLGLSYGSSSWYAVLSDFSVIRKGAVLAVSSSLLAS
                     LAIGEQVDPEALGGWKLHAETTGFADQVVETDAEALAAIRTFLSYLPSHANEAPPERP
                     IPPGSGADMGAIVDLLPVKRTEVYDVRRIIRAIVDTESYFELKPRFGKVAVTALARLG
                     GRSVGIVANNPLVKAGALDVDACEKITSFLVLCDSFNIPIVMLVDTPGFVIGTEAERR
                     RAPGKIMNFMQALQMVSMPKISVILRKTYGQAYLNMGGGRNSDEVAAWPTAEISFMDP
                     TFAVRIVHGIGPGEPGFDTAFERMNRDSEPWDAAGVYAVQTVIAPSETREYLIRMLDV
                     HKLRSSRGVGRHLLAGWPTSY"
     gene            complement(5789..7000)
                     /locus_tag="PK264_09330"
     CDS             complement(5789..7000)
                     /locus_tag="PK264_09330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD76127.1"
                     /translation="MIEIGSTVAGPFCGRMLADFGAEVIKVEPAEGDALRTMGKQYKG
                     HSLYAASMFRSKRLVSIDLNRDEGRAIVLELVAKADVVVENFKPGTLEAWGLGWEQLS
                     RLNPRLVMVRISGYGQDGPYSARPGYGVICEAVSGMRHLTGDPDRPPSRVAVSLTDYI
                     TGLHGAFGAVMALMARGATGRGQFVDAALYECAFNFMEPWIPAYQKLGHVAGRAGSRL
                     AESNPNNLYPTADGEHVHITAMADGLFRKLARAMGRPELATDPRFAKALDRNRHADAL
                     DEIIAGWTLRMPLAEIERILKDAAVPATRIYTIADIFKDPHYTARRSIVEVSDPELGP
                     VAMATVVPRLSGTPGTIRHAGHRVGEDTRTVLAEVLGYDEARIDALAAAGVVTCRGEP
                     MKRLQADEEQA"
     assembly_gap    7002..7018
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7070..7750)
                     /locus_tag="PK264_09335"
     CDS             complement(7070..7750)
                     /locus_tag="PK264_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD76128.1"
                     /translation="MAASKSAARGRRAKVTTSDLVAVLRDRIAGQTLLPGSKLREQDL
                     ADEFSVPRARIRDVFTELAQRGLIERIPNRGAVVTRLDLDQVFQLYDVREVLEGVCAR
                     LATENVPPESWQDLVDHFAGPMVEFVSRGNFDEFIGGYELLRRRTREAAQNPVLGQML
                     DSIYERTAVVIRRIIILPGRAEVGLAEHRAVLAAMRAGDGAEAERLRRHNMRSAKATL
                     HRYRQYVL"
     gene            complement(7967..8791)
                     /locus_tag="PK264_09340"
     CDS             complement(7967..8791)
                     /locus_tag="PK264_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD76129.1"
                     /translation="MSYEAISVEPLSPVIGAEIKGVDLSRPLSNQVRKELHEALLRHL
                     VIFFRDQEIDLDQQKAFGRLFGKLHVHPTAPAPPGHPEVLVIKADEASKAVAGNRWHT
                     DVSCDVEPPMGSILHMRELPPSGGDTMFASMYAAFDALSKPMQKFLEGLKAWHDSEHT
                     LRGTLGQGLKDGVKAYPRALHPVVRTHPETGRKALFVNSGFTTRIDGLGETESRALLD
                     MLFDHVRTPEFQCRFRWREKSVAFWDNRSTQHYALWDYFPHSRLGYRVTICGDRPM"
     gene            9179..>9436
                     /locus_tag="PK264_09345"
     CDS             9179..>9436
                     /locus_tag="PK264_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76130.1"
                     /translation="MPKAYVARHALSRRQALASGGAAGLLAAVGLGGNAQAQGRAPLL
                     RYGLSTWPPNLQPWVSTGVSAGTIKMLTHGRLMRFDATGKLV"
     assembly_gap    9437..9446
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9447..10736
                     /locus_tag="PK264_09350"
     CDS             <9447..10736
                     /locus_tag="PK264_09350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76131.1"
                     /translation="ESVTLLPDGAWQFKLAPNAVFHNGEPVTSADVKYTIEQVAAEKS
                     TAYMRAQFQVVSKVETPDPRTVIIYTKAPVATLPTWFCNYNMPIVSKASTATEAIGCG
                     AYTIAAQERGTSIELKAFDKYFMGGRPRIERLRFVVYADENLRVAALKAGDVDMIEYV
                     PWQSMKSVEEDPRLKLDTQYGPFMDILFNGSKGPLSDVRVRRAIAHAVKREDIVQAAF
                     FGRGKPLEGVPIVEGTPWYDAELARGWRYDPAESRRLLAAAGVPNGFEASMLATAQFG
                     MHKDTAEVVQQHLAAIGIKTELKLPDWATRVRLGTTGQYEIAIHGVAADNNDPDGLSI
                     VMDTSLSPTHGRSFALPCPRTTELFAKGRAELDQTKRVAIYKELQKAAIEEVPLVGLA
                     WRAQGFGMAKKVEGFVNLPGALSLTSGFMLADTRIAG"
     gene            10733..11683
                     /locus_tag="PK264_09355"
     CDS             10733..11683
                     /locus_tag="PK264_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76132.1"
                     /translation="MRYVAGRLAMTLVLAWAVATIVFLTLHLIPGDPAELLLSTGGIT
                     PDPAAVAELRARLGLDRPLLVQYLDFAGRILRGDLGRSLVDDFPVASEIALRLPRTLE
                     LIAAGTAIALLLGIPAGVYAAVHRDGTFDRIMSGVTALLLSIPVFVVGTLLVLVFAQI
                     WRLMPAGGFVPFLSDPARHVTLLALPAIAIGKGLVAIVFRMTRSSVLDALSRDHVRTA
                     RAKGLTPFQVLSRHVVRNSLTPVLTVLGLHMGALLGGTVLVEYVFNWPGLSTPLIRAV
                     EARDYPMVVGIVLTVSGLFLLINLAVDLLYAALDPRARSS"
     gene            11680..13536
                     /locus_tag="PK264_09360"
     CDS             11680..13536
                     /locus_tag="PK264_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HRD76133.1"
                     /translation="MRRWPLRLTVPGALVAVLILIALAVPLLGLPDPIKQDVPQRLLG
                     GIPGSPLGRDEFGRDVLSRLLWGARTSLYVAFAAAAIACLVGTTLGLLGGWLRGLAEI
                     MTLRVADVVLCFPPILLALLVVSVLGPGDRTLVVMLSILYTPGFMRVAFGEVLSTRNL
                     DYVTAVSALGAGTSRILARSILPNIAGPLLVQFSLTLAAAVVVESGLSFLGLGVVPPT
                     PSWGLMIRGARATMEQAPLLLLWPCLALTVTILAINLLCDALRDWLDPRTAPAVGATR
                     GLLDRLAPGLLPSSDIRAPGLKVEGLTVAIATPQSVIRPVEDVSFEVPAGGTLAIVGE
                     SGSGKSLTALALIGLLPPVARAVKGRAMLDGENLLTLDEAALRRLRGNRIAMIFQDPM
                     SSLNPVRTAGAQVIEAILAHRQMAKAAARAEAIALFKRVGIADPERRIDAFPHELSGG
                     MRQRVMIAMAIANKPALLIADEPTTALDVTVQAQILELLAELRRETGASLIFISHSLG
                     VVSEIADHVVVMYAGEVVESGPVALVFEVPLHPYTKALLAAVPDGERAPKPIPGTVPP
                     PDRFPAGCRFRERCASAIEACAERQVLAHTGDGHRVACAVAMSPDAARGAVA"
     gene            13533..14510
                     /locus_tag="PK264_09365"
     CDS             13533..14510
                     /locus_tag="PK264_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD76134.1"
                     /translation="MSVAIPIAGARGVSVTFGSRGLLGRGAVVKAVSSVDLAIARGEV
                     VGLVGESGSGKSTLGRLLLGLLRPTAGEVRFDGLPLARQSREEMRALRRRMQIIFQDP
                     YGSLDPRRRIGAQIADGLAIHGLAPKESMTARVIALLRSVGLDETHADRYPHEFSGGQ
                     RQRVGIARALATRPDFLVADEPVSSLDVSIQAQILDLLAALRRELGLAMLFISHDLPV
                     VRHMSDRVAVMYLGRIMEIGPAEAIFRKPRHPYTQALNSATPHIRSDQRSRRLILKGD
                     PPGPANPPSGCPFRTRCPHAIDACATEVPALQKIADNHAVACRRLNEIG"
     gene            14558..15391
                     /locus_tag="PK264_09370"
     CDS             14558..15391
                     /locus_tag="PK264_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRD76135.1"
                     /translation="MMNVTNPKVHMGMISGGEARELYATNPIILLPMGSHEDQGPHSA
                     MGDYLLAEKIAELAAVKATAAGTRTLVAPVLPFGGADFFGPMIGGIAITPATLEAVIT
                     DMVASLHRTGLTRLIVINGHGGNVGPIAEVARRVYRDTKHVIPSLYLWRIAYGLMPGF
                     LGAEAANKVAGHGADPLTSLGLHLMPELVAKDRIPVAMPLKKDPELGLQFTGLGTASF
                     DGAEVGMPHEYDVTYHAGVAKGDPRLSSAETGRILTEKLVDITARFAAHFAARIPAKN
                     G"
     gene            15403..16080
                     /locus_tag="PK264_09375"
     CDS             15403..16080
                     /locus_tag="PK264_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRD76136.1"
                     /translation="MTDYRIGPMPPQISPALVAKLQKVETATVGHWRHWGFMDRGIQP
                     LLKSKRIAGTAVTLAIPGPDSTLLHHALGLLRPGDILIVDRLGDNRHACWGGGVTVAA
                     KAAGAVAGIVDGPCTDELEIQQSDFPMWCRGLAPITTRLYNLGGIFNGLISCGGVAVQ
                     PGDAVLADESGVLVLPATEADVVAEAALARQERGEKNQERVRKGEKLGDLSGATRMVS
                     EALKATR"
BASE COUNT         2573 a         5665 c         5158 g         2685 t
ORIGIN      
        1 tctggccaac cctcgcagcc cgctgacacc gatatcagga gctcccatgt ccgccgccat
       61 cgctttcgac cagttgcgcc tgccgcctga gtgccagacc ctccgcaagg agatccgcgc
      121 ctttcttgcc gaggaggagg ccgcgggcac cttcgtgcgg ggctccgtcg aggatctcta
      181 cagccctgag ttctcgcgca aggtcggcgc acgcggctgg atcggcatga cctggccgaa
      241 gaagtacggc ggccgcgagc gctcctatct cgagcgcttc gtcgtcaccg aggagttccg
      301 cgtggcgaat gctcctgtgc gccgccactt cgtcgccgac cgccagtcgg gcccggtgat
      361 cctcaagtac gcgcccgagc acatcaagtc ggacatcctg ccgcgcatct gccgtggcga
      421 ggtcggcttc gccatcggta tgagcgagcc gggctcgggc tccgacctct ttgccgccaa
      481 gaccaaggcc acgcccgtga agggcggctt cctcgtcaac ggcacgaagg tgtggacctc
      541 gatggcccac cacgccgact acatgatcgg cctcttccgc acctcgcagc cgaccaagga
      601 gaaccggcgc cacggcctca cccagttcct ggtcgacatg aaggtgaagg gcatcaaggt
      661 caacccgatc taccagatga ccggcctcca cgacttcaac gaggtcgtgt tcgaggacgt
      721 gttcatcccg gaggatcgcg tgctcggcga ggtcgacggc gcctggaagc aggcgacctc
      781 cgagctcgcc tacgagcgga gcggcccgga gcgcttcctc gagaccttct acgtgctcct
      841 gagcctcgtc cgcgccatcg gcgagaagcc ggatctgcgc gccgcggaag gcatcggccg
      901 gctcgtcgcc caggtgcaca ccctgcggcg catgtccgtc tccgtcgccg gcatgctcgc
      961 cgaagggaag gagccggtgc tcgaggcggc gatcgtgaag gatgtcggta cgatctggga
     1021 gcagcgcctc ccaggccgcg cacgcgaact tgccgccttc atcgaggacg aggttgccaa
     1081 ccgcgccact ttcgaggagc agctgcagtt cgcgacgctg gtcgcgccga agctgaccat
     1141 ccagggcggc acgacggaga tcctgcgcgg catcattgcg cgcgggcttg gcctgcgctg
     1201 accaaggtcg ccaaggcatg gacgccgccc ggtcgacccg acggcgtccg ctaccctcta
     1261 cccggctgat caatcgccct tcaggtcatg cacctcgggg aagaacacct ctttccacga
     1321 tgcgggcttc gacttgatga tgccggccga atgcatgaag tcgacgagcg gcatcatctt
     1381 cttcgggccg agatcgtaga tgaactccgg gtcagagagc gacttgatga cgtcctcgat
     1441 cttttccttg ccgctgacat ccttcacgta gatggccgcg gcgcgtgcac gatccgcatt
     1501 gatgatgccg gtcgcctcgc ggaacgcatc cacaacggcc ttgtacgtct tggggttggc
     1561 atcacggaac gcgcccgtcg accacacgga gaggaacgag gtcggcccgc ccatgacatc
     1621 gtaggagctc agcaccttgt gcatgccctt gtcgatggcg cgatactgga acggcggcgc
     1681 caggaacgag gccgagacct cgccacccga gagcagcgcc gcgagcccgt cgggatgcga
     1741 caagttcacg aacagcggat tgagcttctt gaagttggcg atcccgaact ccttggcgac
     1801 cgcgatctgc agatagagcg tctggatgga ctggcccgcg cccgccatcg agatgcggtc
     1861 attctccttg aagtccttca cgctcttgta gggatgccgg ctcacgagga acactgggag
     1921 agcgcagagc gcaccgacgc cgtgcatgcg caccgagccg cgcgtgcggt cccacgccgt
     1981 caccatcggc gcgacgcctc ccgagccgaa atggatcgtt ccggcgatca gcccgtcgtt
     2041 gatcggcacg gcattgccga gcacggacca cttggtcttg aggttcggca tgccggcctt
     2101 ggcggcatgc ttctcgatca gcttctcgtg cagcatgatc gtcagcggca ggtagccgat
     2161 gccgaactgc cggacgagat gcacctcgtc cacctcggcg cgcgcggtcg tcgccgcgcc
     2221 tgcgatcgtt gccgcgagca gccccgctgc cgcgacaccc ttgtaccaag ccgtcatgcg
     2281 cagtcctccc gttcccggat tgtcgtcgtt gtgagcttct acggtctgac gccgtacttg
     2341 ggcttcggcc cgaggctcgt cctgcgacgg gcccgggatg cagccatgaa gcggcaaaga
     2401 taggctctcg tctcgcgcgg gtcgatgata tcctcgacac cgaaggcctc ggcggtgcgc
     2461 atgggtgacg ccttggcgcg aagctcggcc tcgagctcgc gctcgcgggc cttggggtcg
     2521 cgcgccgccg cgatctcgcg ccggaacgcc gcagcgacgc cgccctcgat cggcagcgac
     2581 ccccactcgg ccgagggcca ggcgaccttg aactcgagcc cgttcttgtc ggtggcaccc
     2641 atgccggcca tgccgtagca cttgcgcacg acgacgctca tcatcggcac gctcgcctgc
     2701 aggccgacgt agacggcgcg catgccctcg cgcagcgtgg ccgccatctc ggcatcccgc
     2761 ccgaccatta agcccggcac gtcgacgaag aagacgagcg gaatgtggaa gcagtcgcag
     2821 agctcgacaa aatgcgcctg tttcctcgcg gccctcacgt ccatggcgcc gccgtagacc
     2881 atcggattgt gggcgacgat tccgacaacc tcgccattga gccgggcgag cgccgtgatg
     2941 agcgccctac cgaaggtcgg ctgaatctcg aacagcgagc cccggtcgac gacgagatcg
     3001 acgaggcggc gcatattata gggccggcga cggtccttcg gcacgatacg gagaagctcg
     3061 tcctctgcgc ggcccgcggg atcgttggcg gcgatcgcgg gcggcagctc ccagacattg
     3121 cccggcatgt aggagagaaa gcgccggatg agcgcgaagc actccgcctc gctcgtcgca
     3181 cgattgtcga tcgtgcctgc ctggtcgacg gcgaggccgg gcccgccgag ttcctccttg
     3241 ctgatcttct gaccgaggct cctttcgacg acaggcggtc cagccgcgaa gatctggctc
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgagaggatc gcgcgacccg
     3361 ccgggccgcc tgccgccgtg ccgagcacgg cgcacacgac cggaaccttt cccatcaact
     3421 cgacggaacg ctcgaagccg tgcacgcccg ggaacaccgc atgcccgcgc cgcgtgatag
     3481 aggcgacgct accgcccgcg ccgtcgatga ggttgaccag cggaatgcgg tagctcgccg
     3541 ccatgtcctc gacgaagccg ccctgcccgc ccttcttgcg atcgcccgac cagctgacgc
     3601 cgccgcggat cgtgaagtcc tcgccgccga tcgcaaccgg tcggccgtcg atctcggcga
     3661 gccccatcac gtagggcgcc ggggtcacgc cgacgagccg gtcaccgtca tagcgtccct
     3721 gaccggtcaa acctccgatc tcatggaacg agcccggatc ggcgagcccc gcgatccgct
     3781 cgcggatggt gagccggccc tgctcgtggt ggcgcgcaac cgcggcgtct cctccgagcc
     3841 gggccgcctc agcccgccga aaggccagct cctcgagctc ggcagaccaa tcttccgcag
     3901 ccggcggtgt ttttggctga tcgttcaagg gatgctcact cggcggaggg tctcaactct
     3961 cgagcacggc caccacctgg ccctcggaca cagtatcgcc gaccgccacc agcacctgct
     4021 tcagggtcgc aggatcatcg acgacgacgg ggatctgcat cttcatcgac tcgacgaaga
     4081 gcacggtcga tcccgcctcc agccgatcgc cggccgatgc ctcgatcttg gcgatgatac
     4141 cggtcacctc gctgcgcgcc tcgatctttg ccatgctcgc gtccttccct cgttgccagg
     4201 tctcagtagc tcgtcggcca gccggcgagc agatgccgcc cgaccccgcg gctcgagcgc
     4261 agtttgtgca cgtcgagcat gcggatcaag tactcgcgcg tctcggaggg tgcgatcact
     4321 gtctgcacgg catagacgcc ggcggcatcc cacggctcgc tgtcgcggtt catgcgctcg
     4381 aatgccgtgt cgaagccagg ctcgcccggc ccgatgccgt gcacgatcct caccgcgaac
     4441 gtcgggtcca tgaatgagat ctcggccgtc ggccaggccg cgacctcgtc cgagttccgc
     4501 ccgccgccca tgttgagata ggcttgtcca taagtcttgc gcaggatgac cgagatcttc
     4561 ggcatcgaga ccatctgcag ggcctgcatg aagttcatga tcttgccggg cgcgcggcgc
     4621 cgctccgcct ccgtgccgat gacgaagccc ggcgtatcga ccagcatgac gatcgggata
     4681 ttgaagctgt cgcagagcac gaggaagctc gtgatcttct cgcaggcgtc gacgtcgagt
     4741 gcgccggcct tcacgagcgg gttgttggcg acgattccga cgctccggcc gccaagccgc
     4801 gcgagcgcag tcacggccac cttaccgaag cgcggcttca gctcgaagta gctttccgtg
     4861 tcgacgatcg ctcgaatgat gcggcggacg tcgtagacct cggtccgctt cacgggcagc
     4921 agatcgacga tcgcgcccat gtcggcgccc gaccccggcg gtatcggccg ctcgggcggc
     4981 gcctcgttgg catgcgaggg aaggtaggag aggaacgtgc gaattgccgc cagtgcctcg
     5041 gcatcggtct cgacgacctg atcggcaaag cccgtcgtct cggcgtgcag cttccaccct
     5101 ccgagcgcct ccggatccac ctgctcgccg atcgccagag aggcgaggag cgagctcgac
     5161 accgccagca ccgcgccttt gcggattacc gagaaatcgg aaagcacggc gtaccaactc
     5221 gacgagccgt aggagaggcc aagcgtcgcc gacgcccacg gcgtctcgcg ctggcgtaca
     5281 tactgcgtcg gatcattgcc gagcagcgtt gccatgcccc gcgcgcccat gtggtcgggc
     5341 atgcgcgcgc cggacgactc gccgagaaag acgagcggca gcccccgcgt cgtggcgacc
     5401 cgcttcatgt gcgcgatctt acggccgttg gtcgacgacg acgaggcacc catcaccgtg
     5461 aagtcgttcg agacgacggc gacttcacgc ccctcgatgc gcccgaagcc cgcgaccttg
     5521 ccgtctgccg gcgaccgatc gcgatcctcg ggtcgtgagg aggagacacc gtgcaggcca
     5581 gactcgatga acgagcccgc atcgagcaag ccatcgagac gctcgcgggc atcgagcacg
     5641 ccggccttgg ccttgcgtcg cgttagcttt tccgccccgc ccatggcgag cgcctgcgtg
     5701 cggcggcggc cgagcgatgc aatccgctca ccgtgcgata ccgcagcttt cgcttccggc
     5761 ggtttccctg gcttctttcc cgtctcgctc acgcttgctc ctcgtccgcc tgaaggcgct
     5821 tcatcggctc gccgcggcag gtgaccacgc cagccgcggc aagcgcgtcg atccgcgcct
     5881 catcgtagcc gagcacctcg gcaagcactg ttcgcgtatc ctcgccgacg cggtggccgg
     5941 catgccggat ggtgcccgga gttccggaga ggcgcggcac caccgtcgcc atcgcgactg
     6001 gcccgagctc gggatcgctc acctcgacga tggaccgccg cgccgtgtag tgcgggtcct
     6061 tgaagatgtc ggcgatcgtg tagatgcggg tcgcgggcac ggcggcgtct ttcagaatgc
     6121 gctcgatctc ggcgagcggc atgcgaagcg tccaacccgc gatgatctcg tcgagcgcgt
     6181 cggcatgccg gttgcgatcg agtgcctttg caaatcgcgg gtccgtcgcg agctcgggcc
     6241 ggcccatggc gcgggcgagc ttgcggaaga ggccgtccgc catcgctgtg atgtggacgt
     6301 gctcgccatc cgcagtcgga tagagattgt tcggattgct ctcggcgagc cgagagccgg
     6361 cccgccccgc gacatggccg agcttctggt aggccgggat ccacggctcc atgaaattga
     6421 aggcgcactc gtagagtgcg gcgtcgacga actgcccgcg tcccgtcgcg ccccgcgcca
     6481 tcagcgccat gacggcgccg aaggcgccat gcagcccggt gatgtagtcc gtgagcgaga
     6541 cagcgacgcg cgagggcggc cggtccggat cgcccgtcag gtgccgcatg ccgctcacgg
     6601 cttcgcagat gacgccatag cccgggcgcg ccgaataggg cccatcctgg ccatagcccg
     6661 agatgcgcac catgacgagg cgcgggttca ggcgcgagag ctgctcccag ccgagacccc
     6721 acgcctcgag cgtgccgggt ttgaagttct cgacaacgac gtcggccttg gcgaccagct
     6781 cgagaacgat tgcccggccc tcatcgcggt tgaggtcgat cgagacgagc cgcttgctgc
     6841 ggaacatgct cgccgcgtag agcgagtgac ccttgtactg ctttcccatc gtgcgcagcg
     6901 cgtcgccctc ggccggctcg accttgatca cctcggcccc gaaatcagcg agcatgcgcc
     6961 cgcagaacgg tcccgcgacc gtcgatccga tctcgatcac gnnnnnnnnn nnnnnnnncg
     7021 gtgagcggcc cgcacccaag tgtgcgttca ccgattgcgg gcgcggcgct cacagcacat
     7081 actgccggta gcgatgcagc gttgccttcg cgctccgcat gttgtgccgg cgaaggcgct
     7141 cggcctccgc accatcacca gcgcgcatcg ccgcaagcac ggcacggtgc tcggcgaggc
     7201 cgacctcggc gcggcccggc aggatgatga tacgccggat gacgacggcg gtgcgctcgt
     7261 agatgctgtc gagcatctgc ccgagcaccg gattttgcgc cgcctcgcgc gtgcgcctgc
     7321 gcagcaattc gtatccgccg atgaattcat cgaaattgcc gcggctgacg aattcaacca
     7381 tcggacccgc aaaatgatcg acgaggtcct gccagctctc cggcggcacg ttttcggtgg
     7441 cgagccgcgc gcagacgccc tcgagtacct cgcgcacgtc atagagctgg aacacctggt
     7501 cgaggtcgag ccgcgtcacg accgcgccgc gattggggat gcgctcgatc agaccgcgct
     7561 gagcaagttc cgtgaagaca tcgcggatgc gggcgcgcgg caccgagaac tcgtcggcga
     7621 ggtcctgctc gcgcagtttc gatccgggga gaagcgtctg gcccgcgatg cggtcgcgca
     7681 gaacggcgac gagatcggac gtcgtcacct ttgccctgcg cccgcgcgcc gccgacttgc
     7741 tcgcagccac gaccgctcgt ccagccaagc tcgctcccga ggcggccctg ccacgatagc
     7801 gcaatcgtac cactcgctgg ctcatgccgg gccactctct cttcacgcca cggtcgagcc
     7861 tagtgccgcc gacaagattg tcaacaatcc tgcgggcacg atcgcgcgat cggcaccgtg
     7921 gaatgcggaa cactcgcagg gcgcctcagc tgacgtggcg cccgacttac atcggcctgt
     7981 cgccgcagat tgtcacgcga tagccgagcc gggagtgggg gaaatagtcc cagagcgcgt
     8041 agtgctgcgt cgagcggttg tcccagaacg cgactgattt ttcgcgccag cggaagcggc
     8101 actggaactc gggcgtgcgc acgtggtcga acagcatgtc aaggagcgcg cggctctccg
     8161 tctcgccgag cccgtcgatt cgggtcgtga aacctgaatt cacgaacagc gccttccggc
     8221 ccgtctcggg atgcgtgcgc acgacgggat gcagcgcccg cggatacgcc ttcacgccgt
     8281 ccttgagccc ctgcccgagc gtgccgcgca gcgtatgctc ggagtcatgc caggctttca
     8341 gtccctcgag gaatttctgc atgggcttcg aaagcgcatc gaaagccgca tacatgctcg
     8401 cgaacatcgt gtccccgccc gagggtggca gctcgcgcat gtgcaggatg ctgcccatcg
     8461 gcggctcgac gtcgcaggat acgtcagtgt gccagcgatt tcccgcgacc gccttcgagg
     8521 cctcgtccgc cttgatgacg agaacttccg ggtgtccagg cggcgccggc gccgtcgggt
     8581 gcacatgcag cttgccaaac agccgcccga acgccttctg ctggtcgaga tcgatctcct
     8641 ggtcgcgaaa gaagatcacc agatgacgga gcagcgcctc gtgcagctcc ttgcgcacct
     8701 gattggagag cgggcgagag aggtcgacgc ccttgatctc tgcaccgatc acgggagaga
     8761 gcggctcgac tgagatcgcc tcgtagctca tagacgcctc cacagctgcc tgatgcgccc
     8821 aacggtattt cattggcttg tgagggcaag tgcctagtgt ggcggttcgg aagttcgtat
     8881 tcactgtgta gcactctcaa gatacgaact tctgagcatt gtgccacact aagctcatcc
     8941 aattcctggt gtccttatcg atcgcgaagt tcggctgagt tcgcgactcg cggtagcgaa
     9001 cttcgcgatc gggacactag gcgccgcgat actcgatctc ctgacctgcg aattcgtccg
     9061 ggccggcacc aacgagcgtg ccgaccttgc gctgacgccc cgcgatgtgg tcacctcaac
     9121 ccgacgcgcc aggaacgcca ccagacctgg catcgccgtc gatcgggagg attgagcaat
     9181 gccaaaggca tacgtcgcgc gtcacgcgct ttcccgcagg caggccctgg cctcgggcgg
     9241 cgcggccggt ctcctcgccg cggtcggcct cggcggcaat gcccaagcac aaggacgcgc
     9301 accgctcctc cgctacgggc tttccacctg gccgccgaat cttcagccct gggtcagcac
     9361 cggcgtctcg gccggtacga tcaagatgct gacgcacggc cgcctcatgc gcttcgacgc
     9421 gacggggaag ctcgtcnnnn nnnnnngaga gcgtcacgct gctccccgac ggggcctggc
     9481 agttcaagct cgccccgaac gccgtcttcc acaacggcga gccggtgacc tcggccgacg
     9541 tcaaatacac gatcgagcag gtcgcggccg agaagtcgac ggcctacatg cgcgcgcagt
     9601 tccaggtggt gagcaaggtc gagacgcccg acccgcgcac ggtgataatc tacaccaagg
     9661 cgcccgtggc gacgctgccg acctggttct gcaactataa catgccgatc gtctcgaagg
     9721 cgtcgaccgc gactgaagcg atcggctgcg gcgcctacac gatcgccgcc caggagcgcg
     9781 gcacgtcgat cgagctcaag gccttcgaca agtacttcat gggcggccgt ccccgcatag
     9841 agcggttgcg cttcgtcgtc tatgcggacg agaacctgcg cgttgccgcc ctcaaggccg
     9901 gcgacgtcga catgatcgag tacgtgccct ggcagtcgat gaagtcggtc gaggaggacc
     9961 cgaggctcaa gctcgacacg cagtacggcc cgttcatgga catcctcttc aacggctcca
    10021 agggtccgct ttcggacgtc cgcgtgcgcc gcgcgatcgc gcacgccgtg aagcgcgagg
    10081 acatcgtgca ggcagctttc ttcggccgcg gcaagccgct cgagggtgtg ccgatcgtcg
    10141 aaggcacacc ctggtacgac gccgagcttg cacgcggctg gcgctacgat ccggcggaat
    10201 cgcgccgtct cctcgccgct gccggcgtgc ctaacggctt cgaggcgagc atgctcgcaa
    10261 cggcccagtt cggcatgcac aaggataccg ccgaggtcgt gcaacagcac ctcgcggcga
    10321 tcggcatcaa gaccgagctg aagcttcccg actgggcgac gcgcgtccgg ctcggcacga
    10381 ccggccagta cgaaatcgcc atccacggtg tcgccgccga caacaacgat ccggacggcc
    10441 tgtcgatcgt catggatacg tcgctctcgc cgacgcacgg ccgctcgttt gcgctgccct
    10501 gcccgcgcac gaccgagctc ttcgccaagg gacgcgccga acttgaccag acgaagcgcg
    10561 tcgcgatcta caaggagctg cagaaagcgg ccatcgagga agtgccgctc gtcgggctcg
    10621 cctggcgtgc gcaaggcttc ggcatggcca agaaggtcga gggcttcgtc aatctgccgg
    10681 gcgccctctc gctgacgtcc ggcttcatgc tcgccgatac gaggatcgcc ggatgagata
    10741 cgtcgccggc cgcctcgcca tgacgctcgt gctggcgtgg gcggtcgcga cgatcgtgtt
    10801 cctcacgctg catctcatcc cgggcgaccc ggccgagctg ctcctctcga cgggcggcat
    10861 cacaccggac ccggccgccg ttgcggagct gcgagcgcgg ctcggcctcg atcggccgct
    10921 cctggtgcaa tacctcgatt tcgccggccg catcctccgc ggcgacctcg gccgctcact
    10981 ggtcgacgac tttccggtcg cgagcgagat cgcgctccgc ctgccgcgca cgctcgagct
    11041 gatcgcggcg ggtaccgcga tcgcgctgct gctcggcatt ccggccggcg tctacgccgc
    11101 ggttcatcgc gatggcacat tcgatcgcat catgtcgggt gtgactgcgc tgctgctctc
    11161 gatcccggtg tttgtggtcg gcacgctgct cgtgcttgtc tttgcgcaga tttggcgcct
    11221 catgccggcg gggggcttcg tgccgttcct cagtgatcca gctaggcacg tcaccttgct
    11281 cgcgctgccc gccatcgcca tcggcaaggg cctcgtcgcg atcgttttcc gcatgacgcg
    11341 ctcctccgtc ctcgacgcac tctcgcgcga ccacgtccgc acggcgcgcg ccaagggact
    11401 gactccgttc caggtgctct cgcgccatgt ggtacgcaac tcgctgacgc cggtgctgac
    11461 cgtcctcggg ctgcacatgg gcgcgctgct cggcggcacc gtgctggtcg agtacgtgtt
    11521 caactggccg ggcctctcga cacccttgat ccgcgcggtc gaggcgcgcg actacccgat
    11581 ggtggtcggc atcgtgctga ctgtctcggg gctgttcctg ctgatcaatc tcgccgtcga
    11641 ccttctctat gcagcgctcg atccgcgggc gaggtcgtca tgaggcgctg gcccctgcgg
    11701 ctcaccgtgc cgggcgcgct cgtggccgtt ctcatcctga tcgcgctcgc cgttccgctt
    11761 ctcggcctcc cggacccgat caaacaagac gtgccacagc ggctcctcgg cggcattccg
    11821 ggctcgccac tcggccgcga cgagttcggc cgcgacgtgc tctcgcggct cctgtggggc
    11881 gcgcggacga gcctctacgt cgccttcgcg gcggcagcga tcgcctgcct cgtcggcacc
    11941 accctgggcc tgctcggcgg ttggctgcgc ggcctcgccg aaatcatgac gctgcgtgtc
    12001 gccgacgtcg tgctctgctt cccgccgatc ctgctcgccc tcctggtcgt cagtgtgctc
    12061 ggccccggcg accgcacgct cgtcgtcatg ctctcgatcc tctacacgcc gggcttcatg
    12121 cgcgtcgcct tcggcgaggt gctctccacg cgcaacctgg actacgtgac ggcggtcagc
    12181 gcccttggcg ccggaaccag ccgtatcctc gcccgctcga tcctgccgaa catcgccggc
    12241 ccgctgctcg tccagttttc gctgacgctc gccgccgccg tcgtggtcga gagcggcctc
    12301 tcgttcctgg ggctcggcgt agtgccgccg acaccctcct ggggcctgat gatccgcggc
    12361 gcccgcgcca ccatggagca ggcgccgctg ctcctgctct ggccgtgcct cgcgctcacc
    12421 gtgacgatcc tcgccatcaa cctactctgc gacgcgctcc gcgactggct cgatccgcgc
    12481 acggcgccgg ccgtgggcgc aacgcgcggc ctcctcgacc ggcttgcacc gggcctgctt
    12541 ccttcctccg acatccgtgc gcctggtctg aaagtcgaag gactgacggt cgcgatcgcc
    12601 accccgcagt cggtcatccg accggtcgaa gacgtctcgt tcgaggtccc agccgggggc
    12661 acgctcgcca tcgtcggcga gagcggctcg gggaaatcgc tgacggcact ggcgctcatc
    12721 ggtctcctcc cgcctgtcgc gcgcgccgtc aagggccgcg ccatgctcga cggcgagaac
    12781 ctgctcacgc tcgacgaggc ggcgctgaga cggctccgcg gcaaccggat cgccatgatt
    12841 tttcaggacc cgatgagcag cctcaacccg gtccggacgg ccggcgcgca ggtcatagag
    12901 gcgatcctcg cccaccgcca gatggcgaaa gccgcggcgc gtgcggaggc gatcgcgctc
    12961 ttcaagcgcg tcggcatcgc cgatccggag cgccgcatcg atgccttccc gcacgagctc
    13021 tcgggcggca tgcgccagcg cgtcatgatc gccatggcca tcgccaacaa gcccgcgctg
    13081 ctgatcgccg acgagccgac gacggccctg gacgtcaccg tgcaggcgca gatcctcgag
    13141 ctgctcgccg agctgcgtcg cgagacgggc gcctcgctga tcttcatctc gcatagcctc
    13201 ggtgtcgtct ccgagatcgc cgaccatgtc gtcgtcatgt acgcaggaga agtggtggaa
    13261 agcggccccg tggccctcgt cttcgaggtc ccgctgcatc cctatacgaa ggcgctgctc
    13321 gccgccgttc cggatggcga gcgcgcgccc aagccgattc caggcaccgt gccgccgccg
    13381 gaccgcttcc ctgcgggctg ccgtttccga gagcgctgcg cgagcgcaat cgaggcctgc
    13441 gcggaacgcc aggtgctcgc ccacacaggc gacggccacc gcgtcgcatg tgcggttgcg
    13501 atgtcgccgg acgccgctcg cggagccgtc gcatgagtgt ggcgatcccg attgccggag
    13561 cacgaggcgt gtccgtgacg tttgggtcgc ggggcctgct cggccgggga gcggttgtca
    13621 aggccgtgtc gtcggtcgat ctggccatcg ccagaggcga ggtcgtcggg ctcgtcggcg
    13681 aatccggctc gggcaagagc acgctcggcc gcctccttct cggcctgctg cgcccgacgg
    13741 caggggaagt gcgcttcgac gggttgcccc tcgctaggca gtcgcgcgag gagatgcgcg
    13801 cgctcaggcg gcgcatgcag atcatcttcc aggaccccta tggcagcctc gacccgcggc
    13861 ggcggatcgg cgcccagatc gcagacggcc tcgctattca tggcctcgcg ccaaaagaga
    13921 gcatgaccgc gcgcgtcatc gcgctgttga ggtcggtcgg cctcgatgaa acccacgccg
    13981 accgataccc gcacgagttt tcgggcggac agcgtcaacg cgtcggcatt gcgcgcgcgc
    14041 tcgcgacccg ccccgatttc ctagttgccg acgagcccgt gagctcgctc gatgtctcga
    14101 tccaggcgca gatcctcgac ctgctggcag cgctccgccg cgagctcggc ctcgccatgc
    14161 tcttcatcag ccacgatctt ccggtcgtac gccacatgtc ggaccgcgtt gccgtgatgt
    14221 accttggtcg catcatggag attggccccg cggaggcgat cttccggaag ccgcgccatc
    14281 cctatactca ggcgctcaac tcggcgactc cgcacatccg gtccgaccag cggagccggc
    14341 gtctcatcct gaagggcgat ccgccgggcc ctgccaaccc accgtccggc tgccccttcc
    14401 gcacgcgctg cccgcatgcg atcgatgcct gcgccacaga ggtgcctgcc ctgcagaaaa
    14461 tcgcggacaa ccatgccgtc gcatgccgca gactgaacga gatcggctga catcgccgaa
    14521 agcgaccaac gagacgagac ccgagggaga tgccgccatg atgaacgtca ccaatcccaa
    14581 agtgcacatg ggcatgatct cggggggcga agcgcgcgag ctatacgcga ccaatccgat
    14641 catcctgctg ccgatgggca gccacgagga ccaggggccg cattcggcca tgggtgacta
    14701 tctgctcgcc gagaagatcg cagaactcgc agcggtcaag gcgacggccg cgggcacccg
    14761 cacgctcgtt gccccggtgc tcccattcgg cggcgccgac ttcttcggtc cgatgatcgg
    14821 cggcatcgcg atcacgccgg cgacgctcga agccgtcatc acggacatgg tcgcctcgct
    14881 gcatcggaca ggactcacac gcctgatcgt catcaacggt cacggcggca acgtcggtcc
    14941 catcgccgag gtcgcccgcc gcgtctatcg tgacacgaag cacgtgatcc cgagccttta
    15001 tctttggcgc atcgcctatg gcctgatgcc gggcttcctc ggcgccgagg ctgccaacaa
    15061 ggtcgcgggc cacggcgccg atccgctgac gagcctcggg ctccatctga tgcccgagct
    15121 cgttgccaag gaccgcatcc ctgtcgccat gcccttgaag aaggatcccg agctcggcct
    15181 ccagttcacc gggctcggta cggcgagctt cgatggcgcc gaggtcggga tgccgcacga
    15241 gtacgacgtg acctatcatg ccggtgtcgc caagggcgat ccgcgtctct cgagcgcgga
    15301 gaccggccgc atcctgaccg agaagctcgt cgacatcacc gcgcgtttcg cggcccattt
    15361 cgcggcacgc attccggcga agaacggctg aggagcagcg acatgaccga ctatcgcatc
    15421 ggccccatgc cgccgcagat ttcgccggcc ctcgtcgcca agctgcagaa ggtcgagacg
    15481 gcgaccgtcg gccactggcg ccattggggc ttcatggatc gcggcatcca gccccttctc
    15541 aagtcgaagc gcattgcggg caccgccgtg accctcgcga tcccggggcc ggattcgaca
    15601 ctgttgcacc acgcgctagg actgctgcgg ccgggcgaca tcctgatcgt cgaccgtcta
    15661 ggcgacaatc gccacgcctg ctggggcggt ggcgtgacag ttgccgcaaa agcagcgggc
    15721 gccgtcgctg gcatcgtcga cgggccgtgt accgacgagc tcgagatcca gcaatcggac
    15781 tttcccatgt ggtgccgcgg cctcgcgccg atcaccacgc ggctctacaa tctcggcggc
    15841 atcttcaacg ggctcatctc gtgcggcggc gttgcggtgc agccaggcga tgccgtgctg
    15901 gcggatgaat ccggcgtcct cgtgctgccg gcgaccgagg cggacgtcgt tgccgaggcc
    15961 gcgctcgcgc gacaagagcg cggcgagaag aaccaggagc gcgtcaggaa gggcgagaaa
    16021 cttggcgatc tttccggtgc aacgcgcatg gtcagcgaag cgctgaaggc cacaagatga
    16081 gcaggcccgc gccgcgcggc agacgcgcca ccgaattctc gtgcgagaag cgccccgcga
    16141 ccggctc
//
LOCUS       DAORNH010000071        15929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2807_length_15929_cov_2.654781, whole genome shotgun sequence.
ACCESSION   DAORNH010000071 DAORNH010000000
VERSION     DAORNH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15929
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2807_length_15929_cov_2.654781"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1488
                     /gene="mtaB"
                     /locus_tag="PK264_09380"
     CDS             214..1488
                     /gene="mtaB"
                     /locus_tag="PK264_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRD76137.1"
                     /translation="MSSLDVITLGCRLNAYETEVMRQHATAAGLGDAVIVNTCAVTSE
                     AVRQAERTIRKLRRERAGARIIVTGCAAQIEPERFAAMAGVDHVIGNGEKMRAETFLA
                     LATSASERVQVDDIMSVKETASHLLEGFGSRARAYVQVQNGCDHRCTFCIIPFGRGPS
                     RSVPAGEVVAQVRRLVEAGYPEIVLTGVDITDYGKGLPGDMTLGRLVRQILRFVPELP
                     RLRLSSIDSVEADHDLVTALAEEPRLMPHLHVSLQAGDDLILKRMKRRHSRAEAIAFC
                     AEARRIRPDVAFGADLIAGFPTETDEMFARTLDLVEACDIAFVHAFPFSPRDGTPAAR
                     MPQVARPVVKARAERLREAGRLALACHLERQVGRTVEIVAESGKRGRAADFSTVSLDR
                     AVEPGRLGLAAITAHDGQRLIGRLAETSGATT"
     gene            1485..2627
                     /gene="ftsY"
                     /locus_tag="PK264_09385"
     CDS             1485..2627
                     /gene="ftsY"
                     /locus_tag="PK264_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRD76138.1"
                     /translation="MSEPGAKPRGLLGRLFGRAASETRPAEAPPAEGQGPDLAHQAAT
                     TAPTSVPAPPAAPPRLIEPDPASRAETAAPEKKQGWFQRLKSGLAKTSSRLSDGITSL
                     FTKRKLDGATLDDLEDLLIQADLGVETAMRITERLSKGRFEKGISADEVRQILSSEVE
                     KVLAPVARPLVIEASRRPHVILVVGVNGTGKTTTIGKLAHQFTGAGHKVTIAAGDTFR
                     AAAIDQLKIWGERTGAPVIARDVGADASGLAFDALKSAKESGSDVLLIDTAGRLQNKT
                     ALMDELEKIVRVLRKIDPSAPHDVLLVLDATTGQNALSQVEVFRERAAVSGLVMTKLD
                     GTARGGILVAIAAKHGLPVHAIGIGEGVDDLQPFEPADFARAIAAS"
     gene            2681..3328
                     /locus_tag="PK264_09390"
     CDS             2681..3328
                     /locus_tag="PK264_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HRD76139.1"
                     /translation="MSRSRFRYPFTADQTVNIASEMGPLVAMFIVNGITGNISAGTWA
                     LILSTVAALATSLIVLGRPPIMPFIAGAVSITFGVLSLVTGDPMWVQIKVTIFNTIVA
                     VGLAWGLKQGWNFFEFVFGKTFHYTREGWTKLTRNAAWFFLATAVFNEVIRLGFDAES
                     LYHFDLFNLVLRGIDIWILFKLFLVMPLSAAFMAHQVWRLQHHRLPAPSSAGPET"
     gene            3449..4066
                     /gene="ispZ"
                     /locus_tag="PK264_09395"
     CDS             3449..4066
                     /gene="ispZ"
                     /locus_tag="PK264_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HRD76140.1"
                     /translation="MTTSDREKPATGSSDDPAPWLAESSTGQSWKSVIDFAPLLAFIL
                     AHLYGGLYWATGTLMAAMVLLLPVAYVVLGNISNTQLVTAVLVWVFGALTFWFHDTTF
                     IKLKPTVFNLAFAAFLAGGAWLGKPVLKLLFGEIFRLTEEGWKILTWRWVGFFCSMAL
                     INEIVRMVASDTLWANFKVFGILGLTIAFSIAQLGLLKKYALPSK"
     gene            4476..4901
                     /locus_tag="PK264_09400"
     CDS             4476..4901
                     /locus_tag="PK264_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD76141.1"
                     /translation="MHLLHRVGQCAADLFASEISENDLTPRQYAVLVTVAGNEGLSQT
                     GLVERTGIDRSTLADIVRRMLKKGLLQRRRTREDARAYAVRLTDEGWRVLRSAEPLVK
                     RVDEQILGSLPAKQRTQFVDELKAIIDRMARTDLAPQKG"
     gene            5128..6042
                     /locus_tag="PK264_09405"
     CDS             5128..6042
                     /locus_tag="PK264_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76142.1"
                     /translation="MADGSGKPEIVRRAEAAAAEAASEAKAEVLADENVAPPAKARRR
                     PHAEISATADPWHRFVLDVWITNLNSVEFGFYKHSRNKAKSRQFTSDMDIFAEIVENG
                     ERTGLLGYREDLWSKNTGMDKRLVLKLFGERLNWHATMDLMIGRSLQLTLGARGLPVV
                     AYSANVGNHDHMVYLERSANKWPLMPENFSFFLMDEGKPEFYRLKRRVIDLGGDYALI
                     DQHGREIGGVDGRLLSLGGKWKGRILREHADKNLLLVLKLFCGMLIFNRSAKRHLARL
                     ARDVMSGRYDPKLEKQEADLYMNPRRVR"
     gene            complement(6073..6498)
                     /gene="soxX"
                     /locus_tag="PK264_09410"
     CDS             complement(6073..6498)
                     /gene="soxX"
                     /locus_tag="PK264_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HRD76143.1"
                     /translation="MLGVALAAADLAPAEAPLARFAVTGDAIDAPLDGLVGDAARGRR
                     IIANRDVGNCFACHRAPEPLERFQGNLGPDLAGVARRLTRGQIRLRLVDASLVNPRTI
                     MPPYHRVSSLTRVAPSLRGRPILTAQEIEDVIAYLSTLD"
     gene            complement(6691..8922)
                     /locus_tag="PK264_09415"
     CDS             complement(6691..8922)
                     /locus_tag="PK264_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD76144.1"
                     /translation="MIRNLDPKSLSRRSFVIGAAASGGGLAIGFNLATPGALAQRAGT
                     KTAAAKSAAEVPEVNAWVLIRPNDDVVVRIARSEMGQGTLTGLCQLVAEELECDWAKV
                     KAEYPTPGMNLARKRVWGDMSTGGSRGIRGSHDYVRKGGAAARLMLLTAAAADMKVPV
                     GELTVAKGVITHAASRRRTTYGRVAAAAARLTPPDPATIVLKDPKAWTIAGKPMKRLD
                     TAPKVDGSLVYAIDLKLPGMLNAAIRDCPHFGGRLKSYDGAKVKGMPGVKHVLEIGGT
                     ALAVVADTWWEAKVALDAANIVWDAGPNASVTSATIHTLLMDGLDAKENVFVANKAGD
                     APATLAAAPAAKKIVADYGSPFLNHATMEPMNCTAKWTPEKCEIWVASQNGDASLAAC
                     AAAAGLKPEQCEVYKHHLGGGFGRRGMQDYVTKAVLAAKQVPGVPIKMIWSREEDMVH
                     GRYRPISVARMSGALDEKGNLVALHMRLSGPSILAAVRPEGLQSGMDPLHFQGLHAAA
                     NNPFGQIGYAIPNLTIDHAMRNTHIPPGFWRGVNHNQNAVYLECFIDELAKLAGRDPL
                     EFRRAMMAGHPKHLAVLNAAAAKAEWTKPLPTGVSRGVCQHMGFGSYCAAVAEVSMKA
                     NNKVKVHRLVIAVDSGIALNPEQIAHQVEGSVVYGLGATFYQQCTVKGGRIVEENFDT
                     FPCLTLAEMPKVETVLVPSGGFWGGIGEPTIAVAAPAVLNAIFAATGKMPRTLPLSLH
                     KLV"
     gene            complement(8931..9401)
                     /locus_tag="PK264_09420"
     CDS             complement(8931..9401)
                     /locus_tag="PK264_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD76145.1"
                     /translation="MPKINVNGRTVDVTADPSTPLLWVIREKVGLTGTKYGCGIGQCG
                     SCAVHVNGKVERSCALPLSSVKATDKIVTIEGLSRNASHPIQKAWLALDVPQCGYCQS
                     GQIMAAAALLAVKPKPTDADIDAAMQNICRCGTYQRVRAGIKMAATLVPAGKKA"
     gene            complement(9508..9771)
                     /locus_tag="PK264_09425"
     CDS             complement(9508..9771)
                     /locus_tag="PK264_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76146.1"
                     /translation="MSAAAETDGRDEPDIERLVLASVDDEWRRVAIVIARTIDAARSV
                     GLTVEAGRIAVAIVALAEAGRLMHRGNLRRWRAGEVRRRSAAG"
     gene            complement(9768..10826)
                     /locus_tag="PK264_09430"
     CDS             complement(9768..10826)
                     /locus_tag="PK264_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76147.1"
                     /translation="MTKRVALIAGATGAASRRLVDVLLADPNWTVLGISRRAPAERRE
                     RFVHVAADLLDGASLASKGTEIGAVTHTFYTSRGPFKEGGVEDVPGNVAMLRHLVDAL
                     EAHAPNLEHIHLVEGTKWYGMHLGPTVSPHREDAPRHLPPNFYYDQQDLLAAHQAGKR
                     WTWSASRPEFIYDYAPERARNVVSLIGVWAAICRELGTDLDFPGKPGCFSALTEMTDA
                     TLLARAMVHLSTSPKAANQAFNVTDGDHFRWQHFWPRIAAHFGMRPGIVRPMPLATWM
                     ADKGPVWQRIAEKHGLKASPIADPSIWSFGDFLWRQDGDLLSSIVKIRQTGFGEAIDT
                     EANILAQLTRYREARLLP"
     gene            complement(10843..11664)
                     /gene="fabI"
                     /locus_tag="PK264_09435"
     CDS             complement(10843..11664)
                     /gene="fabI"
                     /locus_tag="PK264_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRD76148.1"
                     /translation="MTGNLMAGKRGLVMGVANNRSIAWGIAKACASQGASLAFTYQGD
                     ALKKRVVPLAEEVGSDIVLPCDVSDPASMDAVFAELQRRWGKLDFLVHAIAFSDKAEL
                     DGRYVDTTEKNFTQTMLISCYSLTALAKRAEPLMAGGGSILTLTYYGAEKVMPHYNVM
                     GVAKAALEASVRYLAADLGKGGIRVNAISAGPIKTLAASGIGDFRYILRWNEYNSALR
                     RTVTIEEVGASGLYLLSDLGRAVTGEVHHVDAGYHIQGMKNEDAPDISVLKGEPG"
     gene            complement(11858..13102)
                     /locus_tag="PK264_09440"
     CDS             complement(11858..13102)
                     /locus_tag="PK264_09440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD76149.1"
                     /translation="MQTDTIVLGAGMVGVSIALHLQKRGRKVALIDRRGVAEETSYGN
                     AGIIQSEANEPYMFPRREWGKILAYALNRTSEANLHYSALPRILMPMLRFYLASTPEK
                     TAAAARAVVALARHCVSEHEALATEASAMALLRRTGYLVLHRDQAALDADIQKRDRIM
                     REYGVPSEPLDAAGVRRLEPHLHGQFTGGMHITDPVSVADPGGLGKAYGDLFVKRGGT
                     LLRGEARTLKRTPTGWEVQTAIGPVTAGEAVVALGPWADVVLSPLGIKLPLFVKRGYH
                     MHYRATGNASLGRPVIDAANGYALAPMMSGIRLTTGAEFALRDAPPTPVQLAKVEPKA
                     RELFPLADRTEPDAWLGRRPCLPDMLPVIGPATGHPNLWLAFGHQHWGFTLGPVTGRL
                     IAEMMTGASPLTDPTPYRMNRF"
     gene            complement(13144..13840)
                     /locus_tag="PK264_09445"
                     /pseudo
     CDS             complement(13144..13840)
                     /locus_tag="PK264_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
     gene            complement(13871..15274)
                     /gene="lpdA"
                     /locus_tag="PK264_09450"
     CDS             complement(13871..15274)
                     /gene="lpdA"
                     /locus_tag="PK264_09450"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRD76150.1"
                     /translation="MAAKFDYVVIGSGPGGYVSAIRAAQLGMSVAVVEKRATFGGTCA
                     NVGCIPSKALLHASEMYHAAGHDFARMGIAVAPALDLPRMLAFKDETVAGNTAGVAFL
                     FKKNKITTFHGLGRIAAPGRVEVTLAAGGTETIEAKHIVIATGSDVAKLPGLAIDETR
                     IVSSTGALTLKEVPKSLVVVGAGVIGLELGSVWRRLGAAVTVVEYLDRILPGMDLEIA
                     KTFQRLLGKQGFQFKLASKVTGAVDKGGRLAVTVEPAKGGAAETLEADVVLVAIGRVP
                     YTEGLGLESVGVKLDERHRIVIDRHFATSVPGIYAIGDVIAGPMLAHKAEDEGLALAE
                     QLAGQAGHVNYDVIPGVIYTSPEVASVGKTEEELKAAGIAYAVGKFSFMGNGRAKVTR
                     ATDGMVKVLADAATDRVLGVHIIGPNAGELIAEAALLMEFGGSAEDMARTCHAHPTLA
                     EALKEAALAVAKRAVHM"
     gene            complement(15405..>15929)
                     /locus_tag="PK264_09455"
     CDS             complement(15405..>15929)
                     /locus_tag="PK264_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRD76151.1"
                     /translation="VGRARTAAFMEVYAHEPGTNPYELEIALVDSLKTGEIPVFACSN
                     PARVAPWGELLSTAARVRGAAGALMDGCTRDVRAIRAMKFPVFHGGIAPLDSKGRGRI
                     MAIDVPIECAGVRVEPGDLIFGDADGVVVVPKRAEAEVLHHALAKVEGERRTLADLEA
                     GEPLKDVFAKYGIL"
BASE COUNT         2632 a         5122 c         5427 g         2748 t
ORIGIN      
        1 gaccgcaagg tcgccgtgga gctgcccggc ggcacgctcg agatcgactg gcgcgcggac
       61 gaccacatct atatgaccgg cgctgccgag ctcgagtacg aggatgtcgt cgatctcgcc
      121 gcagccgcca ctccggtgca agccggttag acgccctgcg gtccgcagca atccgtttca
      181 ccagtcccac tgaggcgtca cgatctggct cccatgtcct cgctcgatgt catcacgctt
      241 ggttgccggc tcaacgccta cgagaccgaa gtcatgcgcc agcacgcgac cgcggccggc
      301 cttggcgatg cggtgatcgt caacacgtgc gcggtgacgt cggaggcagt gcgccaggcc
      361 gagcggacga tcaggaagct ccgccgggag cgcgccggcg cgcgcatcat cgtgacaggt
      421 tgtgctgccc agatcgagcc ggagcgtttt gctgcaatgg ccggcgtcga ccacgtcatc
      481 ggcaacggcg agaagatgcg ggccgagaca ttcctcgcct tggcaacctc cgccagtgag
      541 cgcgtgcagg tcgacgacat catgtcggtg aaggagacgg catcgcatct cctcgagggc
      601 ttcggctcgc gcgcgcgcgc ctacgtgcag gtgcagaacg gctgcgacca ccgctgcacg
      661 ttctgcatca ttcccttcgg ccgcggcccc tcgcgctcgg tgccggcggg cgaggtcgtt
      721 gcccaggtgc gccgcctcgt cgaggccgga taccccgaga tcgtgctcac cggcgtcgac
      781 atcaccgact acggcaaggg gctccccggc gacatgacgc tcggccggct tgtccgtcag
      841 atcctgcgct tcgtgccgga gctgccgcgc ctgcgcctct cctcgatcga ttcggtcgag
      901 gctgaccacg atctcgtcac agcgctcgcc gaggagccgc gactgatgcc gcatctgcat
      961 gtgtcgctgc aggcgggcga cgacctcatc ctgaagcgca tgaagcggcg tcacagccgt
     1021 gccgaggcga tcgcattctg cgccgaggcg cgcaggatcc ggcccgatgt cgcattcggg
     1081 gccgacctca tcgcgggttt tccgacagag acggacgaga tgttcgcgcg gacgctcgat
     1141 ctcgtggagg cctgcgacat cgccttcgtg cacgcctttc cgttctcgcc gcgcgacggc
     1201 acgccggccg cgcgaatgcc gcaagtggcg cgtcctgtcg tgaaagcgcg ggcggagcgg
     1261 ctgcgcgagg caggccggtt ggcactcgcc tgtcatctcg aacgccaggt cggccggacg
     1321 gtcgagatcg tcgccgagag cggcaagcgt gggcgcgccg cggatttttc gaccgtgagc
     1381 ctcgatcggg ccgtcgagcc cggccggctc gggttggcag ccattacggc gcatgacgga
     1441 cagaggctca tcggccgtct ggccgagaca agcggggcaa cgacgtgagt gaaccaggcg
     1501 ccaagccgcg aggcctactc ggacgcctgt tcgggcgtgc cgcgtccgag acccgcccgg
     1561 cggaggcgcc gcccgctgag gggcagggcc ccgatctcgc gcatcaggcc gcaaccaccg
     1621 cgccgacctc ggtgccggca cctccggctg ccccgcccag gttgatcgaa cctgaccctg
     1681 cgtcccgtgc cgagacagcc gcccccgaga agaagcaagg ctggttccaa cgcctgaaga
     1741 gtggcctcgc caagacgtcg tcgcgcctct ccgacggcat caccagcctc ttcacgaaac
     1801 gcaagctcga cggtgctacg ctcgatgatc tcgaggacct gctgatccag gctgatctcg
     1861 gggtggagac ggcgatgcgc attacggagc gattgtcgaa gggccggttc gagaagggca
     1921 tttcggcgga cgaggtgcgc cagattctgt cgagcgaggt cgagaaggtg ctcgcgccgg
     1981 tcgcccggcc cctggtgatc gaggcctcgc ggcgcccgca cgtcattctg gtcgtcggcg
     2041 tgaacggcac cggcaagacg acgacgatcg gcaagctcgc acatcagttc acgggcgcgg
     2101 gccacaaggt gacgatcgcc gccggcgaca ccttccgcgc cgcggcgata gaccagctga
     2161 agatctgggg cgagcggacc ggcgcgccgg tgatcgcgcg cgacgtgggc gccgatgcct
     2221 cggggctcgc gttcgatgcg ctgaagtcag cgaaggagag cggctcggac gtgctgctca
     2281 tcgacaccgc cggtcgcctt cagaacaaga cggcgctgat ggacgagctc gaaaagatcg
     2341 tgcgcgtcct ccgcaagatc gatccgagcg cgccgcacga tgtgctgctg gtgctcgatg
     2401 cgacgacggg gcagaacgcg ctgtcgcagg tcgaggtgtt ccgcgagcgc gcggccgttt
     2461 cgggtctggt gatgacaaag ctcgatggca cggcgcgggg cggcattctg gttgccatcg
     2521 cggccaagca tggactgccg gtgcacgcga tcggcatcgg agagggggtg gatgatctgc
     2581 agcctttcga gcccgcggat ttcgcacgcg cgattgcagc gagctgaagt ctgcggctac
     2641 aacatcggta acgaaagcgt gaagggatgg cacgcgacgc atgagccgca gtcgatttcg
     2701 atatccattt acggccgatc agacggtcaa catcgcgagc gagatgggac cgctcgtggc
     2761 gatgttcatc gtcaacggaa ttaccgggaa catctctgcg ggtacctggg cgctgatatt
     2821 gtcgaccgtt gcggcgctcg caacatcgct catcgtgctc ggtcggccgc cgatcatgcc
     2881 gttcatcgcc ggcgcagtca gcataacgtt cggcgtcctc tcgcttgtca ccggtgaccc
     2941 gatgtgggtg cagatcaagg tgaccatctt caatacgatc gtcgccgtcg gtctcgcctg
     3001 gggcctgaag cagggctgga acttcttcga gttcgtgttc ggcaagacct ttcactacac
     3061 ccgcgagggc tggacgaagc tcacgagaaa tgctgcatgg ttcttcctgg cgacggccgt
     3121 cttcaacgag gtcatacggc tcggcttcga tgccgagagc ctctatcact tcgatctgtt
     3181 caatctcgtg ctgcgcggca tcgacatatg gatccttttc aagctgttcc tggtcatgcc
     3241 tttgagcgcg gccttcatgg cccatcaggt ctggcgcctg cagcatcatc gtctgccggc
     3301 cccatcctca gctggaccgg agacatgagg cagtcgttgc cgcggaccag cgattggggc
     3361 tatcccaggt ggtgacgtag cgccggtcgc gcgccgtggc cgaggtagcg gagcacggcc
     3421 ggcgggggcg ggaaggatca tcggcgcaat gacgacatca gatcgagaga aacctgcgac
     3481 aggatcatcg gacgatccgg cgccctggct cgccgaatcg tctactggcc aatcatggaa
     3541 atcggtcatc gatttcgccc ctctgttggc gttcatcctt gctcatctct acggcgggtt
     3601 gtattgggcg accggtacac tgatggctgc catggtgttg ctgctgccgg tcgcttatgt
     3661 cgtgctcgga aacatctcca atactcagct ggtcacggcg gtgctggtat gggtgttcgg
     3721 cgcactgacc ttctggtttc acgacaccac gttcatcaag ctgaagccga cggtgttcaa
     3781 cctggccttc gcagcattcc tcgcgggtgg tgcctggctc ggcaagcccg tgctgaaact
     3841 gctgtttggc gaaattttcc ggctgacaga ggagggctgg aagattttga cgtggcgttg
     3901 ggtgggattc ttttgcagca tggcgctgat caacgaaatt gttagaatgg ttgcctccga
     3961 tacgctgtgg gcaaatttca aagtctttgg aatactcggc ctgacgattg cgttctcgat
     4021 tgcacagctt ggtcttctga agaagtacgc cttgccgtcc aagtgatcct gtgctgccgc
     4081 gctgcatgtg ccgcgacaca gcgggcgaac cgggttggcc aagctgtata gtgggtatgc
     4141 gcctgacgta tacccacgtc ggaaccgcgc ctgacatggg attgcaaaga agcgatcaac
     4201 atcgtcatcg caaatcgctc aaattacttg ggaaaaatta ggtaaaaatg atccgattac
     4261 tgatgatggg agattaattc agtagtagaa aatttcctac gaagtggaaa ctgctagacc
     4321 ggattatatt cgtggcgtaa tgtctctttc catgcacaaa agaaggctgg cagatcttca
     4381 gggttaggca ggtttcgagc acaatggcga agatgagcga gaaaacagac gcatctcgag
     4441 gtggaactgg cagtgaacgg ttggatcggt cgcccttgca cctgctccat cgcgtcggcc
     4501 agtgcgcagc agatctgttt gccagtgaga tcagcgagaa cgatctgacg ccacgtcagt
     4561 atgctgttct cgtgacagtg gctggcaacg aggggctcag tcagaccggg cttgtggaac
     4621 ggacgggcat cgatcgatct accttggccg acatcgtccg acgcatgctg aagaaaggcc
     4681 tgctgcagcg ccggcggacg cgcgaggatg cgcgcgccta tgcggtgcgc ctcacggacg
     4741 aaggctggcg cgtgcttcgg tccgccgagc cgctcgtcaa gcgtgtcgac gagcagatcc
     4801 tcggctcgct gccggcgaag caacgcacgc agttcgtgga tgagcttaag gcgatcatcg
     4861 atcgcatggc gcggacggac ctcgcccctc agaagggcta agcgtcgcgc atgactgcct
     4921 cgcgatcggc cggtgctcag agcgccggcc gatcgcgttt cagggggcgg gagaagctcc
     4981 cccgagaggg agtgctgggt gagacttgtc cgggcttttt ccaccgcctg ctgcacaggc
     5041 cgcggccgcc gactgagagc aacatgctag cgtctcactc gattcgtgcc tcgttccgcg
     5101 tcgcgctcaa cgtagagggg gcagcccatg gcagatggaa gtggaaagcc ggagatcgtc
     5161 cggcgcgccg aggcggcagc ggcagaagct gcgagcgagg ccaaggcgga ggtcctggcc
     5221 gatgagaacg tggcgccacc cgccaaggcg cggcgccggc cgcacgccga aatttcggcc
     5281 acggccgatc catggcaccg gttcgtgctg gatgtgtgga ttaccaacct caactcggtc
     5341 gagttcggct tctacaagca cagccgcaac aaggcgaagt cgcgccagtt cacctccgac
     5401 atggatatct tcgcggagat cgtcgagaac ggcgagcgca cgggtctgct cggttaccgc
     5461 gaggacctct ggtcgaagaa tacgggcatg gacaagcgcc tcgtcctcaa gctcttcggc
     5521 gagcgcctca actggcacgc gacaatggac ctgatgatcg gccgctcgtt gcagttgacg
     5581 ctcggcgccc ggggccttcc cgtcgtcgcc tattcggcaa acgtcggcaa ccacgaccac
     5641 atggtctatc ttgagcgctc tgccaacaag tggccgctga tgccggagaa tttctcgttc
     5701 ttcctgatgg acgaggggaa gcctgagttc tatcggctga agcggcgtgt gatcgatctc
     5761 ggcggcgact acgctctgat cgatcagcat ggccgcgaga tcggcggcgt cgacggccgc
     5821 ctcctctccc tcggcggcaa gtggaaaggc cgcatcctgc gcgagcatgc cgacaagaac
     5881 ctgctcctgg tgctgaagct cttctgcggc atgctgatct tcaaccgctc cgcgaagcgc
     5941 catctcgccc gtctcgcccg agacgtgatg tccggccgct atgatcccaa gctcgagaag
     6001 caggaagccg acctctacat gaacccgcgc cgtgtgaggt aggcgtcacg cctgccgagc
     6061 agggcgcgcc gatcagtcga gcgtcgaaag gtaggcgatc acgtcctcga tctcctgggc
     6121 agtgaggatc ggccgcccgc gaaggcttgg tgcgacgcgc gtgaggctcg acacgcggtg
     6181 gtacggcggc atgatcgtcc tcgggttcac gagcgaggcg tcgacgaggc ggagcctgat
     6241 ctggccgcgc gtcaggcggc gggcgacgcc cgcgaggtcc ggtccgaggt tgccctgaaa
     6301 gcgctccaaa ggctcaggtg cccggtgaca ggcgaagcag ttgccgacgt cccggttggc
     6361 gatgatgcgc cggccgcggg cggcatcgcc gacgaggccg tcgagcggcg cgtcgatcgc
     6421 atcgcccgtc acggcaaagc gcgcaagcgg cgcttcagcc ggcgcgagat cggccgcagc
     6481 gagcgccacg cccagcatga gacctgcaat gcagatcgcg ccaactcgcc tgagggcagg
     6541 cctgtcgcgc atacggagcc acgaaaggtt tgacctttcg tggcggagta tcgcgcgcgg
     6601 ggttggcggg gcgaccgcgc gcaccaaagg agtgataccg gccaactgca cagtagctca
     6661 cacacaggtc aatggatcag ttggccggta tcagacgagc ttgtgcaggc tcaggggcag
     6721 cgtgcgcggc atcttgcctg ttgccgcgaa aatcgcgttg agcacggcgg gagctgcgac
     6781 cgcaatcgtc ggctcgccga tgccgcccca gaagccgccc gagggcacga gaacagtctc
     6841 gaccttcggc atctcggcga gtgtcaggca tgggaaggtg tcgaagttct cctcgacgat
     6901 ccggccgccc ttcaccgtgc actgctggta gaaggtcgcg ccgaggccgt agaccaccga
     6961 gccctcgacc tgatgcgcga tctgctcggg gttgagcgcg atgccactgt cgaccgcgat
     7021 caccaggcga tgcaccttca ccttgttgtt cgccttcatc gacacctcgg cgacggcggc
     7081 gcagtagctg ccgaagccca tgtgctgaca cacgccgcgg gacacgcccg tcggcagcgg
     7141 ctttgtccat tcggccttcg ctgcagccgc attgaggacg gcgaggtgct tcgggtggcc
     7201 cgccatcatg gcacggcgga actcgagcgg gtcgcggcct gcgagcttgg cgagctcgtc
     7261 gatgaagcac tcgaggtaga ccgcgttctg gttgtgattg acccctcgcc agaagcccgg
     7321 cgggatgtgc gtgttgcgca tcgcgtggtc gatcgtgagg ttcgggatcg catagccgat
     7381 ctggccgaat ggattgttgg ccgccgcatg cagcccctgg aagtgcagcg ggtccatgcc
     7441 gctctgcagg ccctcggggc gcaccgctgc caggatcgac gggccggaga ggcgcatgtg
     7501 gagggcgacg agattaccct tctcgtcgag tgcgcccgac atgcgcgcca ccgagatcgg
     7561 ccggtagcgg ccgtgcacca tgtcttcctc gcgcgaccag atcatcttga tgggcacgcc
     7621 cggcacctgc ttcgccgcga gcacggcctt cgtgacgtag tcctgcatgc cgcggcgccc
     7681 gaagccgccg ccgaggtgat gcttgtagac ctcgcactgc tcgggcttca ggccggccgc
     7741 cgccgcacag gcggcgagcg aggcatcgcc attctgcgag gcgacccaga tctcgcactt
     7801 ctccggcgtc catttagccg tgcagttcat cggctccatg gtggcgtgat tgaggaaggg
     7861 cgagccgtag tccgcgacaa tctttttggc ggcgggcgcc gctgcgagcg ttgccggcgc
     7921 gtcgccagcc ttgttggcga cgaacacatt ctccttggcg tcgaggccat ccatcagcag
     7981 cgtgtggatc gtggcgctcg ttacgctcgc gttgggaccg gcgtcccaga cgatgttggc
     8041 cgcatccaat gcgaccttcg cctcccacca cgtgtcggcg accacggcga gggccgtgcc
     8101 accgatctcg agcacgtgct tcacgcccgg catccccttc actttggcgc cgtcgtagct
     8161 cttgaggcgg cccccgaagt gggggcaatc gcggattgcg gcattgagca tgccggggag
     8221 cttcaggtcg attgcgtaga cgagcgagcc gtcgaccttc ggcgccgtgt cgagccgctt
     8281 catcggcttg ccggcgatcg tccaggcttt cgggtccttg aggacgatgg ttgccggatc
     8341 gggcggcgtg aggcgcgccg cggcggcagc gacccggcca taggtcgtgc ggcgccggct
     8401 cgcggcatgc gtgataacac cctttgctac cgtgagttcg ccgaccggca ccttcatgtc
     8461 ggcagcggcg gccgtcagca gcatgaggcg cgcggcggcg ccgcccttcc tgacatagtc
     8521 gtgcgagccg cggatgccgc ggctgccgcc agtcgacatg tcgccccaca cccgcttccg
     8581 cgcgaggttc atgccgggcg tcgggtactc ggccttgacc ttcgcccagt cgcactcgag
     8641 ctcctcggcg acgagttggc agaggcccgt gagcgtgcct tggcccattt ccgagcgtgc
     8701 gatgcggacg acgacatcgt cgttcggccg gatcagcacc caggcattca cctccggtac
     8761 ctcggcagca gactttgctg cagcagtctt ggtgccggcc cgctgggcga gggccccagg
     8821 cgtcgcgagg ttgaagccga tcgccaagcc accgccggat gcggcggcac cgatgacgaa
     8881 cgagcggcgg gagagcgact tcggatcgag attacggatc atggctggcc tcacgccttc
     8941 ttgcccgcgg gaaccagggt cgcggccatc tttatgcccg cgcggacgcg ctggtatgtg
     9001 ccgcagcggc agatgttctg cattgcggcg tcgatgtcgg cgtcggtcgg tttcggcttc
     9061 actgcgagaa gcgccgccgc cgccatgatc tgccccgact ggcagtagcc acactgcggc
     9121 acatcgagcg cgagccacgc cttctggatc ggatgcgagg cgttgcgcga aaggccctcg
     9181 atggtcacga ttttgtccgt cgccttcact gacgacagcg gcagcgcaca cgagcgctcg
     9241 accttgccgt tgacgtgcac cgcgcacgag ccgcactggc cgatgccgca gccgtatttg
     9301 gtgccggtga ggccgacctt ctcgcggatc acccagagca gcggcgttga cgggtccgcc
     9361 gtcacatcga cagtgcggcc attgacgttg atcttcggca tccggacctc cgcaagcgtg
     9421 tggcgagcat ggctgtagag catcgacagc gggatgaagc cccaagccta tgcgagccag
     9481 cggcggtggt cgatcacgaa tacgtgttca gccggcagct gatcggcggc ggacctcgcc
     9541 cgcccgccag cgacggagat tgccgcgatg catgaggcgc cccgcctcgg cgagggccac
     9601 gatggcgacg gcgatgcgcc cggcctccac cgtcaggccc accgaccggg ctgcatcgat
     9661 ggtgcgcgcg atcacgatgg cgacgcggcg ccattcgtcg tcgaccgaag cgagcacgag
     9721 gcgctcgata tcgggctcgt ccctgccgtc ggtttcggct gcggcgctca tggcaagagc
     9781 cgcgcctcgc ggtaccgcgt cagctgggca aggatgttcg cctccgtgtc gatcgcctcg
     9841 ccgaagcccg tctggcggat cttgacgatg ctcgagagga ggtcgccgtc ctggcgccag
     9901 aggaaatcgc cgaagctcca gatcgacggg tcggcgatgg gcgaggcctt gagcccgtgc
     9961 ttctcggcga tgcgctgcca gacggggccc ttgtcggcca tccaggtggc gagcggcatc
    10021 gggcggacga tgccgggtcg catgccgaag tgggcagcga tgcgcggcca gaaatgctgc
    10081 cagcggaagt ggtcgccgtc ggtgacgttg aaggcttggt tggcagcctt cggcgaggtc
    10141 gagagatgca ccatggcgcg ggcgagcagc gtcgcgtcgg tcatctcggt cagtgccgag
    10201 aagcagccgg gcttgcccgg aaaatcgaga tcggtgccga gctcgcggca gatcgcggcc
    10261 cacacgccga tcagcgagac gacgttgcgc gcgcgctccg gcgcgtagtc gtagatgaac
    10321 tcgggccgcg aggcggacca cgtccagcgc ttgcccgcct ggtgagccgc aagcagatcc
    10381 tgctggtcgt aatagaagtt cggtggcagg tggcgagggg catcctcgcg atgtggcgag
    10441 acggtcggcc ctaggtgcat gccgtaccac ttcgtccctt cgacgaggtg gatgtgctcg
    10501 agattcggcg cgtgcgcctc gagcgcgtcg acgagatggc ggagcatggc aacgttgccc
    10561 gggacatcct cgacgccgcc ttccttgaag gggccgcgcg aggtgtagaa ggtatgcgtc
    10621 acggcgccga tctcggtgcc ctttgatgcg aggcttgcgc catcgaggag gtccgctgcg
    10681 acgtgcacga atcgctcgcg gcgctcggct ggtgctcggc ggctgatccc gagcacggtc
    10741 caattggggt cggcaagcag aacatcgacg aggcggcggg aggcggcgcc cgtcgcgccg
    10801 gcgatgaggg cgacacgctt cgtcatgatc gctcagcgct cctcagccgg gctcgccctt
    10861 caggacggag atgtccggcg catcctcgtt cttcatgccc tggatgtgat agccggcatc
    10921 gacgtgatgc acctcccccg tcacggcgcg gccgagatcg gagagaaggt agagccccga
    10981 ggcgcccact tcctcgatcg tcacggtgcg gcgcagcgcc gagttgtact cgttccagcg
    11041 caggatgtag cggaagtcgc cgatgccgga ggcggcgagc gtcttgattg ggcccgccga
    11101 gatcgcgttc acacggatgc cgcccttacc gaggtcggcc gcgagatagc gcacgctcgc
    11161 ctcgagcgcg gccttggcaa ctcccatgac gttatagtgc ggcatcacct tctcggcgcc
    11221 gtagtaggtg agcgtcagga tcgacccgcc gcccgccatc agcggctcag cgcgcttggc
    11281 gagcgccgtc agcgagtagc aggagataag catcgtctgc gtgaagtttt tctcggtcgt
    11341 gtcgacgtat cgcccgtcga gctcggcctt gtccgagaag gcgatcgcat gcaccaggaa
    11401 gtcgagcttg ccccagcggc gctgcaactc ggcaaagacc gcatccatcg aggccggatc
    11461 ggagacgtcg catgggagca cgatgtccga gccgacctcc tcggcgagcg gcacgacgcg
    11521 cttcttcaag gcatcgcctt ggtaagtgaa ggcgagcgat gccccctgcg atgcgcaggc
    11581 cttggctatt ccccaggcga tcgagcggtt gttggcgacg cccatgacga ggccccgctt
    11641 gccggccatc aaatttccgg tcatcggcgc ttggtctcga ggctccatcg ccacacccct
    11701 gggttcaaac gaaaacgatg ccgcgggctg cttccgcggc aggctatgac ggcgccttct
    11761 tgcacaaaag gcgccatggg tccaggcggc tcgcgccgga cggccatgcg cccatggccg
    11821 gcgcgacaca gtcgatgagg atcggcgtga tcaatgatca aaatcggttc atgcgatacg
    11881 gggtcggatc ggtgagcggc gatgcgcccg tcatcatctc ggcgatcaga cgcccggtta
    11941 cgggaccgag cgtgaagccc cagtgctggt ggccgaaggc gagccagagg ttcgggtggc
    12001 ccgtggccgg cccgataacg ggcagcatgt cggggagaca cggtcgacgg ccgagccagg
    12061 catccggctc cgtgcgatcg gcgagcggga agagctcgcg cgccttcggc tcgaccttgg
    12121 cgagctgcac gggggtcggc ggagcatcgc gcaaggcgaa ctccgcgccg gtggtcagcc
    12181 ggatgccgct catcatcggc gcgagcgcat agccgttggc cgcatcgatc accgggcggc
    12241 cgaggctcgc attcccggtt gcgcgatagt gcatgtgata gccccgcttc acgaacagcg
    12301 gcagcttgat accgagaggg gaaagcacga cgtcggccca gggcccgagg gcgacaactg
    12361 cctcacccgc tgtcacgggg ccgattgcgg tctgcacctc ccagcccgtc ggcgtgcgtt
    12421 tgagggttcg ggcctcgccg cgcaggagcg tgccgccgcg cttcacgaag aggtcgccgt
    12481 aagcctttcc gaggccgccc ggatcggcga cgctcaccgg atcagtgatg tgcataccgc
    12541 cggtgaactg cccatgcaga tgcggttcga gccggcgcac gccggccgca tcgagcggct
    12601 ctgagggaac tccgtactcg cgcatgatgc ggtcgcgctt ctggatgtcc gcgtcgaggg
    12661 cggcttggtc gcgatgcagc acgagatagc cggtgcgccg gagcagcgcc atggccgatg
    12721 cttccgtcgc cagcgcttcg tgctcgctga cacagtgtcg ggcaagcgcc acgactgcgc
    12781 gtgccgccgc agccgtcttc tcgggcgtcg aggccaagta gaagcgcagc atcggcatga
    12841 ggatgcgggg aagcgcgctg tagtggaggt tcgcctcgct cgtgcggttc aaggcgtatg
    12901 cgaggatctt cccccattcc ctgcgcggga acatataggg ctcgttcgcc tcgctctgga
    12961 tgatgcccgc gttgccgtag ctcgtctctt cggcaacgcc gcgacgatca atgagcgcga
    13021 cctttcgccc gcgcttctgg agatgcaagg cgatcgacac gccgaccatg ccggcgccga
    13081 gaacgatcgt atcggtctgc atgaggcctc tagcggtcgg cgcggggtca gtactgaccg
    13141 gcctcagtgg atctcgtcca tggcgagcag cgccttgcgg agctgatcga tgccgaagcc
    13201 gggctttcgc gccgcatcga gaatcacggc ttccttgcgt gcgagcaggt cgcaggcggc
    13261 cgggatcttc gcggcgagcg cgtgcgggat gacgacggcg ccatgccggt cggcgtgaat
    13321 gaggtcgccc gagcgcacga tcatgccggc gacccgcacc tccttgccat agtcggcgag
    13381 gtggatgtgg gcatggctcg gcgcgatcga gccggcgagc gcctggaacc ccggcgccca
    13441 ctgcggaatg tcgcggatgc agccgtcggt gacgacgccg atgcaaccga ggcccttgtg
    13501 gaccgcgctc tgcacctcgc cccagaaggc gccgaagccg atgtcgtggc cgtcgaggtc
    13561 ctgtagcacg gcgatggtcg ggccgggggc cgaggtactc gtagtagccg acgcgctggg
    13621 ccttcgccgc cttgccgtcg agttcgttgg cgtgcgtcgc gcggatggtc gccgtgcgcg
    13681 cgtagccgac gatcggcggc aggtcgggga aggggcagac gagctgcttc gtcgtgaagc
    13741 cggtcaggcg ccgcgaggca tcgaccacct cgagcgcgtt gcagatggtc ggcgtatcga
    13801 cggctttcag cttctcgatc aggtcggcgg gatgctgcat ggcgggcctc ggcggaagga
    13861 tgatccgccg ctacatgtgc acggcccgct tggcgacggc aagggctgcc tccttcaatg
    13921 cctcggcgag ggtcggatgt gcatggcagg tgcgcgccat gtcctcggct gagccgccga
    13981 actccatcag gagtgcggcc tccgcgatga gctcgccggc gttcgggccg atgatgtgca
    14041 cgccgagcac gcgatcggtc gcggcatccg cgagcacttt caccatgccg tccgtcgcgc
    14101 gcgtgacctt ggcgcggccg ttccccatga acgaaaactt gccgacggca taggcgatgc
    14161 ccgccgcctt cagctcctcc tcggtcttgc cgacgcttgc gacctcgggc gaggtgtaga
    14221 tgacgcccgg gatgacgtca tagttgacgt ggcccgcctg ccccgcgagc tgctcggcga
    14281 gcgccaagcc ctcgtcctcg gccttgtggg cgagcatcgg cccggcgatc acgtcgccga
    14341 tggcgtagat gccgggaacg ctcgtcgcga aatgcctgtc gatgacgatc cgatgccgct
    14401 cgtccagctt cacaccgacg gattcgagcc ctaggccctc ggtgtagggc acgcgtccga
    14461 tggcgacgag cacgacgtcg gcctcgagcg tctcggcagc acctcccttg gcaggctcga
    14521 ccgtcacggc aagccggccg cccttgtcga cagcacccgt caccttgctg gcgagcttga
    14581 actggaaccc ttgcttgccg aggaggcgtt ggaaggtctt ggcgatttcg aggtccatgc
    14641 cggggaggat gcggtcgagg tattcgacga cggtcaccgc cgcgccgagc cgtcgccaga
    14701 ccgagccgag ctcgaggccg atgacgcctg cgccgacgac gacgagcgat ttcggcacct
    14761 ccttcagcgt gagcgctccg gtcgaggaga caatgcgcgt ctcatcgatc gcgaggccgg
    14821 ggagcttggc gacatccgag cccgtggcga tgacgatgtg cttcgcctcg atcgtctccg
    14881 tgccgccggc cgcgagcgtc acctcgaccc gtccaggtgc agcgatgcgg ccgagcccgt
    14941 gaaaggtcgt gatcttgttc ttcttgaaca ggaaggcgac acccgccgtg ttcccggcga
    15001 ccgtctcgtc cttgaaggcc agcatgcgtg ggagatcgag cgccggcgcg acggcgatgc
    15061 ccatcctggc gaaatcgtgc ccggccgcgt gatacatctc ggaggcgtgc agcagcgcct
    15121 tggaggggat gcagccgacg ttggcgcagg tgccgccgaa ggtcgcccgc ttctcgacga
    15181 cggcgacact catgccgagc tgcgccgcgc gaatggccga cacatagccg ccaggtcccg
    15241 agccgatgac gacgtagtcg aatttcgctg ccatgctgag cctcggccga gagtgtgggt
    15301 ctgtgaccgg cgagcttaga gccgcgcttc gccctcgccg tcaagacgga cgcaagtcta
    15361 gcgtcaccgc gaggggcagg cggcgagcgc gtggctcgcg gcacttacaa gatgccgtac
    15421 ttcgcgaaca cgtccttcag cggctcgccg gcctcgaggt cggcgagcgt gcggcgctcc
    15481 ccctccacct tggcgagcgc atggtggagc acctcggcct cggcgcgctt cggcacgacc
    15541 acgacgccgt cggcatcgcc gaaaatgagg tcgcccggct cgacgcgcac gcccgcacac
    15601 tcgatgggca catcgatcgc catgatccgc ccgcggccct tgctgtcgag cggcgcgatg
    15661 ccgccgtgaa acaccggaaa tttcatcgca cggatggcgc gcacgtctcg cgtgcagccg
    15721 tccatgagtg cgcctgcagc cccgcgcacc cgtgccgccg tcgagaggag ctcgccccag
    15781 ggcgcgacgc gggccggatt cgagcaggcg aacaccggga tctcgccggt cttcaaactg
    15841 tcgacgaggg cgatctcgag ctcgtagggg ttggtgccag gctcatgcgc atagacctcc
    15901 atgaaggctg ccgtgcgcgc ccgcccgac
//
LOCUS       DAORNH010000072        15639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2874_length_15639_cov_2.494995, whole genome shotgun sequence.
ACCESSION   DAORNH010000072 DAORNH010000000
VERSION     DAORNH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15639
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2874_length_15639_cov_2.494995"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..>671)
                     /locus_tag="PK264_09460"
     CDS             complement(131..>671)
                     /locus_tag="PK264_09460"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRD76152.1"
                     /translation="SLVAGSDKGAVANTQVFLAIGHDSASGELALERDRIRIVWPGAA
                     REPVYQRIEATLKELVAATGGTYIANPVSQRFLGGNLFTVHPLGGATMADTASSGVID
                     DRCRVFNPGDSEGAVHRGLYVMDGSVVPRSLGVHPLLTITALAERAMLLFARDQGLKL
                     SVEPKAGTPVRSFVTGAKA"
     assembly_gap    672..799
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<800..1850)
                     /locus_tag="PK264_09465"
     CDS             complement(<800..1850)
                     /locus_tag="PK264_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76153.1"
                     /translation="MSIRLAKPVGSLAARYDAVVIGSGYGGGVAASRLSRMGLKVAVL
                     ERGREWLPGEFPSSVLAAQREFRMTGAKLSIGSPTALFDLRLGDDVHVLQASGVGGTS
                     LINANVCLVPDARVIEDHIWPEGVRTNHWLQVGFRRARDMLQPATLPAASTPLKLKAL
                     EKAAKSVSSVAERIPLHITFEETVNAAGVRQPACSECGDCLGGCNVGAKTTVHSTYLT
                     DAVNHGAEIHTHAEVRYLESAGAEGWRITLHRLDDNAKAVPLRRVMARMVVLSAGTLG
                     TNEILMRSRERGLKLSDRLGKRLSTNADAIAFGYNNDQRVNAIGTGSSTKRNGREPRV
                     GPAVAGLIDLRRDRGL"
     gene            complement(1887..3071)
                     /locus_tag="PK264_09470"
     CDS             complement(1887..3071)
                     /locus_tag="PK264_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRD76154.1"
                     /translation="MAAPRLEAVDIFYLSMPVVEDIGDGSQDACVVRVRAGGYEGWGE
                     CEASPLPTIAALVAPMSHSACHPVASAVLGEVIESPDDIRRINRQVRAKCLDLLQAAH
                     TLSGIDMALWDLVGRMRDEPVWKLLGYTRAYPKLPYASVLFGDTAAETLAKARAIAGK
                     GFRAAKFGWGPYGAGTPDADRDHVMAAREGLGPDAALLVDAGTIWVDDVARARKSLPA
                     LQDARATWLEEPFVSGALGAYAALARESGSVRLAGGEGAHDPWMARQMIDHGGIGFVQ
                     IDAGRVGGLSDAKIVADHARAKGVTYVNHTFTSQLALAASIHPFAGIEDDRIAEFPLE
                     ARPVAGAIAVERIEVGPDGLIRLPERPGIGMTVDPRRFKPYLVDTEIKVGGRLLYRTP
                     AV"
     gene            complement(3155..4906)
                     /locus_tag="PK264_09475"
     CDS             complement(3155..4906)
                     /locus_tag="PK264_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018209141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76155.1"
                     /translation="MSSGVDRPSETLLTVRNLSVEFASTAAPARVVKGVNFVIKRGET
                     VALVGESGSGKTVSAHSILRLLPYPLASHPSGEVYFEGLDLLKADAARMATVRGRRIS
                     IIFQEPMTSLNPLHSIEKQVGEILALHDRKGAFEPTGRERAFRFLRSLPLIGRSYANR
                     EELFPAETERRRRVLALLERVGIREAAKRLDAFPHQMSGGQRQRVMIAIALANAPELL
                     IADEPTTALDVTIQAQILALLKELRDEMGMSMLLITHDLGIVQKMADRVYVMQRGEIV
                     EEGTVDQIFGKPTHPYTRQLISAEPKGQAHVADPNAPVIAETDDLKVWFPIKRGFFKR
                     VVDHVKAVDGVSIKVRAGETLGVVGESGSGKTTLGLAVLRLISSKGPIVFMGERIDGF
                     QSKAMRPLRRKMQIVFQDPYGSLSPRLSIGQIIEEGLTILEPGLDYDERRERVGKTLI
                     EVGLDPAMQDRYPHEFSGGQRQRIAIARAMVLNPKFVMLDEPTSALDRSVQAQIVDLL
                     RDIQRRHGLGFMFISHDLKIVRALSNHVVVMRNGKVVEEGSAEEIFNAPKEAYTRALL
                     AAALNLETHAEPGSSAT"
     gene            complement(4920..>5435)
                     /locus_tag="PK264_09480"
     CDS             complement(4920..>5435)
                     /locus_tag="PK264_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD76156.1"
                     /translation="KVDLIFQRILEIWSSMPSLYILIIISAILTQGFWTLLFILLLFH
                     WISLVNVVRAEFLRGRNFEYVTAARALGLSNVRIMFKHILPNAMVALLTYLPFKLSGA
                     ISALTVLDFLGLGLPPGSPSLGELLRQGQDTAAPWLGLSGFFSVALILSLLIFIGEAV
                     RDALDPRKTFT"
     assembly_gap    5436..5475
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5476..6066)
                     /locus_tag="PK264_09485"
     CDS             complement(<5476..6066)
                     /locus_tag="PK264_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76157.1"
                     /translation="MLDRVQTFIRSPLNQRRWSSFKANKRGYWSLWVFLSLFVVSLFA
                     EVIANDRPIIMRYKGELLFPVLVDYPEEKFGGFLARTNYKNPAIYDEITKGDNWAIWP
                     LIRFNGTDSTNNDYPLRKSPDGLCLGFPAPPFWASKLPLCEAPTDQMARYDILSNRNW
                     LGTDDSGRDVLARIIYGFRISVFFGILLTILSSIIGV"
     gene            complement(6131..>6625)
                     /locus_tag="PK264_09490"
     CDS             complement(6131..>6625)
                     /locus_tag="PK264_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD76158.1"
                     /translation="PSRGLWSENWNELSTFGKIKDYFWHLTLPLIAIGLGAFTTMTFL
                     TKNSFLDEIRKQYITTARAKGLSERRILYGHVFRNAMLLVIASFPAQFIGAFFTGSLL
                     IEHIFSLDGLGKLSFEAVRKRDYPIVFASLYIQSLIGLVVAIVTDFIYTRIDPRIDFE
                     TREV"
     assembly_gap    6626..6635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6636..7249)
                     /locus_tag="PK264_09495"
     CDS             complement(<6636..7249)
                     /locus_tag="PK264_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318799.1"
                     /note="with YejAEF is involved in resistance to microcin
                     C; Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin ABC transporter permease"
                     /protein_id="HRD76159.1"
                     /translation="MGAYLLKRLLLIIPTLFGIMLISFIVVQFAPGGPVEQMMAKLQG
                     ADGGSAVGSGPGDGVAGTAGPQTGAGFDSGGSSYRGAQGLEKRFIEELNKQYGFDKPA
                     HERFWIMIKNYLSFDFGRSFFRDEDVIKIILEKLPVSISLGLWMTLLVYLVSIPLGIR
                     KAVRDGEPFDAWTSSLLIVGYAVPGFLVGMFLLVVFAGASFFQW"
     gene            complement(7297..9183)
                     /locus_tag="PK264_09500"
     CDS             complement(7297..9183)
                     /locus_tag="PK264_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76160.1"
                     /translation="MTTHPSLARPLSAILAAVVILGAITQLPASSGAQTPPASGPRHG
                     LSVFGDLKYPADFKHFEYVNPNAPKGGKARFVGLSFDSLNPFILKGDKPYGLGAPVDL
                     VYDSLMTPAADEPGSYYGLIAESADVAPDRKSVIFKMRPEARFADGTPVTSEDVVHTF
                     DLLKTKGHPAYRLSLLKDVVKAEALGPHTVKYSFEGNRLRDLPITVASLAVTPKAFWA
                     KQPFEETFLNVMPLGSGAYRVEKIDGNTSITYRRRDDYWAKDLPVNRGRYNFDEIQYI
                     YIAERSLELQAILAGTYDIREEFTSRDWAAGYNVPAVKEGRLVRVTLPDETPSGTQGF
                     FINTRRAKFADPRVRRALDYAFDFETTNRTLFFGLYERTVSYFENSDLKASGKPSPAE
                     LAVLEPFRASLPPEVFGEVYVPPKTDGSGNDTRNLREAVRLLNEAGFVLKGQKRVNAA
                     GVELSIEFLITDATSERLLNPYVKNLNAIGITATMRRVDTAQYQRRVKEYDFDIANSR
                     FVMSLNPGSELDNYFNSANAKTEGSRNLAGVSSPAVDALVARIGEAKTRDELVAASRA
                     LDRVLRAGHYWVSHWYKGTHHVAYWDKYDRPAIKPKYARAITDTWWFNAEKAAKLRQP
                     CPPM"
     gene            complement(9222..>10790)
                     /locus_tag="PK264_09505"
     CDS             complement(9222..>10790)
                     /locus_tag="PK264_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76161.1"
                     /translation="PSTSYGLVAEWVEVQKDFSAAKFRLRPEAAFHDGRPITPDDVVF
                     TFEALTRNDPKYIDYYKDVAKVEASGEREVTFHFAVKGNRELAHIVGDMPILPRHYWE
                     AKGADGTRRDIAKATLEPPLGSGPYRIKSLEAGRHIVYERVKDWFAAKLPVMRGQWNI
                     DEIKFIYFDDRQVAFNDFKAGNLDYWREPSAKGWATGYDFDAVKRGHVKRSEVKLASL
                     HPMQGFAFNLRRPQFQDWRVRRAFDLCFDFDGLNKKIMFSAYKRVESFFEGSELKATG
                     IPEGREKAILEEIRPLVPADIFTTPYRTPHHTSLTEARANMREAQRLLAEAGWRIDPA
                     STGKPVLKKASGEVLTAEFLIVSRDFERLILPYIKELESLGIHASVRIVDSSQYIKRR
                     SSFDFDIVVASFGQSISPGNEQREFWGSAAADREGTRNIIGIKDAGIDALIEKVIFAK
                     DRVELVAATRALDRLLMRGHYLVPQWYGPVERIAHWDMLGRPETLPARSVAFPRVWWV
                     DPDAQKRLAVARGR"
     assembly_gap    10791..10806
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10807..11169)
                     /locus_tag="PK264_09510"
     CDS             complement(<10807..11169)
                     /locus_tag="PK264_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76162.1"
                     /translation="MLDCAKAGKVARGVGFLRPACAVGVTLALFATTATTPSSRADDA
                     QRRRLHAMSLIGEPAYGPEFTHFKWVNPEAPKGGSIRMFAPGTFDTLNPFTIKGLPAV
                     GLAAPQSLLYATLMSTSPD"
     assembly_gap    11390..11485
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11486..>12574)
                     /gene="ftsH"
                     /locus_tag="PK264_09515"
     CDS             complement(<11486..>12574)
                     /gene="ftsH"
                     /locus_tag="PK264_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRD76163.1"
                     /translation="VEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLG
                     GGNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDRQITVPAPD
                     VIGREKILRVHMRKVPLAPDVDAKVIARGTPGFSGADLANLVNEAALLAARRNKRIVT
                     QSEFEDAKDKVMMGAERRSMAMTEEEKLATAYHEAGHALVNVLMPHSDPLHKVTIIPR
                     GRSLGVTMALPERDKLSYSRKWCESRIAMTFGGRVAEQLIYGEDHLNTGASNDIMQAT
                     DLAKRMVTEWGMSEKLGPLRYTDNQQEVFLGHAITQRQNMSEETAKVIDEEVRRIVGV
                     GYQTARELIYTHKDKLEAITRALMEHETL"
     assembly_gap    12575..12659
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12660..13248)
                     /locus_tag="PK264_09520"
     CDS             complement(<12660..13248)
                     /locus_tag="PK264_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HRD76164.1"
                     /translation="MTPHFRNLAVWVIILVLLAALYNMFQTQPQGRRAGDVSYSEFLN
                     AVEQDKVAEVTITGNRISGVYRDSASRFQTFAPEDPQLVERLRKKDIRFTARPPDEDM
                     PWLLSALISWLPMLLIIGIWLLFMRQMQSGSGRAMGFGKSRAKLLTERQGRVTFEDVA
                     GVDEAKVDLQEIVEFLRDPQKFQRLGGRIPRGCLLV"
     gene            complement(13477..>13758)
                     /locus_tag="PK264_09525"
     CDS             complement(13477..>13758)
                     /locus_tag="PK264_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76165.1"
                     /translation="TAKANLIRRQEETAATRSLLNTARLMDDNPLLLRLKELEALEKL
                     VEKVGRIDLHANGPGGGLDALLRNLYKLSEPKPDADGGPAAAQRIEKKT"
     assembly_gap    13759..13768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13769..14666)
                     /locus_tag="PK264_09530"
     CDS             complement(<13769..14666)
                     /locus_tag="PK264_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HRD76166.1"
                     /translation="MFWFKVSVKDDERGFLLRNGKIERLLPPGRFSAFDPSRELSCEI
                     VKVVRTELPIERALMLSRLHPRIAEENIEIVKTGPTQVGLIALDGQLSTLMTPNSIRA
                     FWTSVTEVTVDLVDAARELRMTKPYQDRLAGWRTPLIVEAMVEPNERALLIVDGRLEE
                     QLAPGRHVFWQVGRTIRVQKFDMRPQALEVTAQEILTKDRIGIRVTLTAFFQVADAEK
                     AMMATGDLGQTIYKHVQFAIREAVAARTLDEILAARDTLDKEVRAYVQPRVGELGCAM
                     GEIGVKDIILPGDVRELLNKVVE"
     gene            complement(15093..15163)
                     /locus_tag="PK264_09535"
     tRNA            complement(15093..15163)
                     /locus_tag="PK264_09535"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            15389..>15639
                     /locus_tag="PK264_09540"
     CDS             15389..>15639
                     /locus_tag="PK264_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76167.1"
                     /translation="MSPRHDYDLFVIGAGSGGVRAARIAASHGARVAIAEEYRVGGTC
                     VIRGCVPKKLLVYASRFHGEFEDAKAFGWTVCGPTDPLRS"
BASE COUNT         2609 a         4722 c         5283 g         2640 t
ORIGIN      
        1 gcgtgatcgc ggctgctaag ccgtgcggcc cggagccgac gaccaccgca tcgtactctg
       61 gcttcagctc cgcaatatcc ctggcgagcc gcatccgtgt ctctccccac tcgtgcaatt
      121 caggaaatga tcaggccttg gcccctgtca cgaagctgcg gaccggggtg ccagcctttg
      181 gctccacact caatttgaga ccctggtcgc gcgcgaacag cagcatcgcc cgctccgcga
      241 gcgcggtgat cgtcagcagc gggtgcacgc cgagcgagcg cggcacgacc gagccatcca
      301 tcacatagag cccgcgatgg acagcgcctt cgctgtcgcc cggattgaac acgcggcagc
      361 ggtcatcgat cacgccgctg ctcgccgtat cggccatggt cgcgccgcca agcgggtgca
      421 cggtgaaaag gttgccgccc aggaagcgct ggctcacggg attggcgatg taggtgccgc
      481 cggtcgccgc gacaagctcc ttcaaggtgg cctcgatgcg ctggtagacg ggctcgcgcg
      541 ccgcgcccgg ccagacgatc ctgatgcgat cgcgctcgag cgcgagctcg ccagaggcgc
      601 tgtcgtgccc gatcgcgagg aacacctgcg tattggcgac ggctcctttg tccgagccgg
      661 caacgaggct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnc gaggccgcga tcccggcgga gatcgatgag ccccgcgacg
      841 gccggtccga cgcgcggctc gcgtccgttt cgcttcgtgc tgctgcccgt gccgatcgcg
      901 ttgacgcgct ggtcgttgtt gtagccgaag gcgatcgcgt cggcgttggt cgagaggcgc
      961 ttgccgagcc ggtcggagag cttgaggccc ctctcgcgcg agcgcatcag gatctcgttc
     1021 gtcccgagcg taccggcgga gagcaccacc atgcgtgcca tgacacggcg gagcggcacg
     1081 gccttggcgt tgtcgtcgag gcgatgcagc gtgatgcgcc atccttccgc gccggcgctc
     1141 tcgaggtagc gcacctcggc gtgcgtgtgg atctcggcgc cgtggttcac ggcgtccgtg
     1201 agataggttg aatgcaccgt cgtcttggcg ccgacgttgc agccgccgag gcagtcgccg
     1261 cactcgctgc aagccggctg cctgacgcct gccgcgttga ctgtctcctc gaaagtgatg
     1321 tgcaggggaa tgcgctcggc cacgctcgaa acggactttg ccgccttttc gagcgccttg
     1381 agcttcaagg gggtggaggc ggccggcagc gtcgccggct gaagcatgtc gcgtgcgcgg
     1441 cggaagccca cctgcagcca atgattggtg cgcacgcctt cgggccagat atggtcctct
     1501 atgacgcgtg cgtccggcac gaggcagacg ttggcattga tgagcgaggt gccgccgaca
     1561 ccggacgcct gcagcacatg cacgtcgtcg ccgaggcgca ggtcgaaaag cgcggtcggc
     1621 gagccgatgc tgagctttgc gccggtcatg cggaactcgc gctgcgccgc gagcacgctc
     1681 gaagggaact ctcccggcag ccactcgcgt ccgcgctcaa gcacagcaac cttgaggccc
     1741 atacgcgaga ggcgcgaggc cgcaacgccg ccgccatagc ccgagccgat gacgacggca
     1801 tcatagcgcg ccgcgagcga gccgaccggc tttgcgagac gaatgctcaa acacggcctc
     1861 ccggcacacg ccgcaccacc ttctcctcag accgccggcg tgcggtagag gaggcgcccg
     1921 ccaaccttga tctcggtatc gacgagataa ggtttgaagc ggcgagggtc gaccgtcatg
     1981 ccgattccgg gccgctcggg caggcggatg agaccatccg gcccgacctc gatccgctcg
     2041 acggcgattg cgccggcaac gggtcgcgcc tcgagcggga attcggcgat cctgtcgtct
     2101 tcgatcccag caaatggatg gatcgaggcc gccagcgcaa gctgcgacgt gaaggtgtgg
     2161 ttgacgtagg taaccccttt cgcccgggca tgatcggcga cgatcttggc atcgctgagc
     2221 ccgccgaccc ggcccgcatc gatctgtacg aagccaatgc ctccatggtc gatcatctgc
     2281 cgcgccatcc aggggtcgtg cgcgccctcc ccacctgcga ggcgcacgct gccgctttcc
     2341 cgcgcgagcg ccgcataggc cccgagcgcg ccagagacga acggctcttc gagccatgtt
     2401 gcacgcgcat cctggagcgc cggcagggac ttccgcgccc gcgcgacgtc gtcgacccag
     2461 attgtgccgg catcgaccag aagtgccgca tcgggcccga gcccttcgcg cgcggccata
     2521 acgtgatcgc ggtcggcatc gggggtgccg gcgccgtagg ggccccagcc gaatttggcg
     2581 gcgcggaacc ccttgccggc gatcgcgcgg gccttggcga gcgtttcagc ggcggtatcg
     2641 ccgaagagga ccgaggcgta ggggagcttc ggataggccc gcgtgtagcc gagcagcttc
     2701 caaaccggct cgtcccgcat ccgaccgacg aggtcccaga gcgccatgtc gatgccggaa
     2761 agcgtgtgcg ccgcctgtag caggtcgaga cacttggcgc gcacctgccg gttgatgcgg
     2821 cggatgtcgt ccggactctc gatcacctcg ccgagcacgg cgctggcaac tggatgacag
     2881 gccgaatgcg acatcggggc gacgagcgcg gcaatcgtcg gcaatgggga ggcctcgcac
     2941 tcgccccagc cttcgtagcc tccagcgcgg acgcgcacga cgcacgcatc ctggctgccg
     3001 tcgccgatat cctcgacgac cggcatggag agatagaaga tgtcgacggc ctcaagtctc
     3061 ggcgcggcca tggtcccctc accctgagcc tgcagactag cgaccaaccc ccgtcttggc
     3121 gagcgcgttg gcagatgttg cgggcgagtg atggtcaggt cgcgctgctg ccaggctcgg
     3181 catgcgtctc gagattgagg gcggcggcga ggagcgccct cgtgtaggcc tccttcggag
     3241 cattgaagat ctcctcagcc gagccctcct cgacgacctt tccgttgcgc atcaccacga
     3301 catggttcga gagcgcgcgg acgatcttca ggtcatggct gatgaacatg aatccgagac
     3361 cgtgccggcg ctggatgtcg cggagcaggt cgacgatctg cgcctgcacg ctgcgatcga
     3421 gcgcgcttgt cggctcatcg agcatcacga acttgggatt gagcaccatg gcacgggcga
     3481 tggcgatgcg ctggcgctgg ccgcccgaga actcgtgcgg atagcggtcc tgcatggcgg
     3541 gatcgagccc cacctcgatg agagtcttgc cgacgcgctc gcggcgctcg tcgtagtcga
     3601 ggccgggctc caggatggtg aggccctcct cgatgatctg tccgatggag aggcgcggcg
     3661 acagcgagcc gtaagggtcc tggaagacga tctgcatctt gcgccggagc ggccgcatcg
     3721 ccttcgactg gaagccgtcg atgcgctcac ccatgaagac gatcgggccc ttcgaggaga
     3781 tcaggcggag gacggcgagg ccgagcgtcg tcttcccgga accggattcg ccgacgacgc
     3841 cgagcgtctc gcccgcgcgc accttgatcg agacgccgtc cactgccttg acgtgatcga
     3901 cgacgcgctt gaagaaaccg cgcttgatcg ggaaccagac cttgaggtcg tcggtctcgg
     3961 cgatgacggg cgcgttcgga tcggcgacgt gcgcctggcc cttgggctcc gccgagatga
     4021 gttggcgcgt ataggggtgg gtcggcttgc cgaaaatctg atcgaccgtt ccctcttcca
     4081 cgatctcgcc gcgctgcatg acgtagaccc gatcggccat cttctggacg atgccgaggt
     4141 cgtgcgtgat gagcagcatg gacatgccca tctcgtcgcg aagctccttc aggagagcga
     4201 ggatctgcgc ctggatcgtc acgtcgagag ccgtcgtcgg ctcgtcggcg atgagcagct
     4261 ccggcgcatt tgcgagtgcg atagcgatca tcacgcgctg gcgctggccg cccgacatct
     4321 ggtgcgggaa ggcatcgagg cgctttgcag cctcgcggat gccgacacgc tcgaggagcg
     4381 cgaggacgcg ccggcgccgc tcggtctcag cggggaagag ttcctcgcgg ttcgcgtagg
     4441 agcggccgat cagggggagc gagcgcagga agcggaaggc gcgctcgcgg cccgtcggct
     4501 cgaacgcgcc ttttcggtcg tgcagcgcaa ggatctcgcc gacctgcttc tcgatcgagt
     4561 ggagcgggtt gagcgaggtc atcggctcct gaaagatgat cgagatgcgg cggccgcgca
     4621 ctgtcgccat gcgcgcggca tcggccttga ggagatcgag tccctcgaaa tagacctcgc
     4681 ccgacggatg cgaggcaagc ggatatggga gaaggcgcag gatcgaatgg gccgaaacgg
     4741 tcttgccgga gccggattcg ccgacgaggg caacggtctc gccgcgcttg atgacgaagt
     4801 tgacgccctt gaccacgcgg gcgggcgccg cggtcgaagc gaactcgacg gacaggttgc
     4861 gaaccgtgag caacgtctcg gatgggcgat ccacgcctga actcatgtga aggtgggcct
     4921 catgtgaacg tcttgcgagg atcgagagcg tcgcgcaccg cctcgccgat gaagatcagc
     4981 agtgagagaa tgagcgccac cgagaagaag ccggagagcc caagccaggg tgcggcggta
     5041 tcctggccct ggcgaagcag ttcgccaagc gaaggcgatc cgggcggcag gccgaggccg
     5101 aggaaatcga ggacggtcag ggccgagatc gcgcccgaga gcttgaacgg caggtaggtg
     5161 agcagggcca ccatggcatt tggcaggatg tgcttgaaca tgatgcgcac attcgagagc
     5221 ccgagcgcgc gggcagcggt cacgtattcg aagttgcgcc cgcgcaggaa ctcggcacgc
     5281 acgacgttga cgagcgatat ccagtggaag agcagcagga tgaacagcag tgtccagaag
     5341 ccctgcgtca ggatcgccga gatgatgatc aggatgtaga gcgacggcat cgacgaccag
     5401 atctcgagga tgcgctggaa gatgagatcg accttnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnncacgc cgatgatgga ggagaggatc gtcagcagaa tgccgaagaa
     5521 gaccgagatg cggaagccgt agatgatgcg ggcgaggacg tcgcggccgg aatcgtcggt
     5581 gccgagccaa ttccggttgg agaggatgtc gtagcgcgcc atctggtccg tcggcgcttc
     5641 gcagagcggc agcttcgagg cccagaaggg gggggccgga aagccgaggc agaggccgtc
     5701 gggcgatttg cgcagtgggt agtcgttgtt ggtcgaatcc gtgccgttga agcggatgag
     5761 cggccagatg gcccagttgt cgcccttcgt gatctcgtcg tagatcgcgg gattcttgta
     5821 gttcgtacga gcgagaaagc cgccgaactt ctcctcaggg tagtcgacga ggacggggaa
     5881 gagcagttcg cccttgtagc gcatgatgat cggccggtcg ttggcgatca cctcggcaaa
     5941 gaggctcacg acgaacagcg acaggaagac ccagaggctc cagtagccgc gcttgttggc
     6001 cttgaagctc gaccagcggc gctggttgag cggcgagcgg atgaaggtct ggacgcgatc
     6061 gaggataccg gtccgccgcc gccgcgcggg cgcggatgtc ggagccgtgg tcggttgcag
     6121 cgctgccacg tcagacctcc cgcgtctcga agtcgatgcg gggatcgatg cgcgtgtaga
     6181 tgaagtcggt gacgatggcg acgacgaggc cgatcagcga ctggatgtag agcgaggcga
     6241 agacgatcgg gtagtcgcgc ttcctcaccg cttcgaatga gagcttgccg agaccgtcca
     6301 gcgagaagat gtgctcgatg aggaggcttc ccgtgaagaa ggcgccgatg aactgggcgg
     6361 ggaagctcgc gatcacgagc agcatggcgt tgcggaagac gtggccgtag aggatgcgcc
     6421 gttccgagag gcccttggcg cgcgccgttg tgatgtattg cttcctgatc tcgtcgagga
     6481 acgagttctt ggtgaggaag gtcatcgttg tgaaggcgcc gaggccgatg gcgatcagcg
     6541 gcagagtgag gtgccagaaa tagtccttga tcttgccgaa ggtcgaaagc tcgttccagt
     6601 tttccgacca gaggccgcgc gagggnnnnn nnnnnaacca ctggaagaac gaggcgccgg
     6661 caaacacgac gagcaggaac atgccgacga gaaagcccgg cacagcatag ccgacgatca
     6721 gcagcgacga cgtccaggca tcgaacggct cgccgtcgcg cacggccttg cggatgccga
     6781 gcgggatcga gaccaggtag acgagcagcg tcatccagag gccgagcgag atcgagacgg
     6841 ggagcttttc gaggattatc ttgatgacgt cctcgtcacg aaagaagctg cgtccgaaat
     6901 cgaagctcag gtagttcttg atcatgatcc agaagcgctc gtgcgccggc ttgtcgaagc
     6961 cgtactgctt gttgagctct tcgatgaagc gcttctcgag gccctgtgcg ccgcgatagc
     7021 tcgagccgcc gctgtcgaag ccggcgcctg tctgcggacc cgcggtcccg gcaacgccgt
     7081 cacccggacc ggagccgacg gccgagccgc cgtcggcgcc ctgcagcttc gccatcatct
     7141 gctcgacggg gccgcccggc gcaaactgca cgacgatgaa gctgatgagc atgatgccga
     7201 acagggtcgg gatgatcagc agcaatcgct tcagcagata ggcgcccatt gcgattgtcg
     7261 tcctgatggc tcgggttcag tcacgcgcgt acctgcctac atcggcgggc acggttgccg
     7321 cagcttggcg gctttctcgg cgttgaacca ccaggtatcg gtgatggcgc gcgcgtactt
     7381 gggcttgatc gcgggacggt cgtacttgtc ccagtaggcg acgtggtggg tgcccttgta
     7441 ccagtgcgag acccagtagt ggccggcgcg cagcacccgg tcgagcgcgc ggctcgctgc
     7501 caccaactcg tcgcgtgttt tcgcttcgcc gatcctggcg acgagcgcgt cgacggctgg
     7561 gctcgagaca ccggcgaggt tgcgcgagcc ttccgtcttg gcattggcgc tgttgaaata
     7621 gttgtcgagt tcgctgccag ggttgagcga catgacgaag cgggagttgg cgatgtcgaa
     7681 gtcgtactcc ttcacgcggc gctgatactg ggcggtatcg acgcggcgca tggtggccgt
     7741 gatgccgatg gcgttgaggt tcttgacgta ggggttcagc agccgctccg acgtcgcgtc
     7801 cgtgatcagg aactcgatcg agagctcgac gcccgcggca ttgacacgtt tctggccctt
     7861 gagcacgaag cccgcctcgt tgaggaggcg cactgcctcg cgcaggtttc gcgtgtcgtt
     7921 ccccgagcca tccgtcttcg gcggcacgta gacctcgcca aagacctccg gtggcaggct
     7981 cgcgcggaac ggctcgagga cggcgagctc ggccgggctc ggcttgcccg aggccttcag
     8041 atcggagttc tcgaagtaac tgacggtgcg ctcgtaaaga ccgaagaaca gtgtccggtt
     8101 cgtcgtctca aaatcgaagg cgtagtcgag cgcccgacgc acgcgcgggt cggcgaactt
     8161 ggcgcgccgc gtattgatga agaagccctg cgtgcccgag ggcgtctcgt cggggagcgt
     8221 cacgcggacg agccggcctt ccttgacggc tggcacattg tagccagcgg cccagtcgcg
     8281 cgaggtgaac tcctcgcgga tgtcgtaggt gccggcgagg atcgcctgca gctcgaggct
     8341 gcgctcggcg atgtagatgt actgtatctc gtcgaagtta tagcggccgc gattgaccgg
     8401 taggtccttc gcccaatagt cgtcgcggcg acgataggtg atgctcgtgt tgccgtcgat
     8461 cttctcgacc cggtaggccc ccgagccgag cggcatcacg ttgaggaacg tctcctcgaa
     8521 gggctgcttc gcccagaagg ccttcggcgt gacggcaagg ctcgcgaccg tgatcggcag
     8581 gtcgcgcagt cgattgcctt cgaaagagta tttgacggtg tgcggaccga gcgcctcggc
     8641 cttgaccaca tccttcaaca gcgacaggcg ataggcgggg tggcccttgg tcttcaggag
     8701 atcgaatgtg tgaacgacgt cctccgaggt gacgggcgta ccgtcggcaa acctggcctc
     8761 ggggcgcatc ttgaagatga ccgatttccg gtccggggcg acgtcggcgc tttcggcaat
     8821 gagaccgtag taggaaccgg gctcgtcggc cgcgggcgtc atgagcgagt cgtagacgag
     8881 gtcgaccggc gcgccgagac cgtagggttt gtcgcccttc aggatgaacg ggttcaggct
     8941 gtcgaaggag aggccgacga agcgggcctt gccacccttg ggcgcgttgg ggttaacgta
     9001 ctcgaaatgc ttgaaatcgg ccggatactt gaggtcgccg aagactgaga ggccgtgccg
     9061 ggggccgctc gctggcgggg tctgtgctcc gctcgaagcc ggcaactgtg tgattgctcc
     9121 gaggatcaca actgccgcga ggatcgccga gagggggcgg gcgagggagg ggtgggtggt
     9181 catgacggct ccagatcgga atggcagtcg aggtgtggtc gtcagcggcc gcgcgcgacg
     9241 gcgagccgct tctgggcatc ggggtccacc caccagacac gggggaaggc cacgctgcgg
     9301 gccggaagcg tttcggggcg cccgagcatg tcccaatggg cgatgcgctc gaccggacca
     9361 taccactgcg gcaccagata gtggccgcgc atcagcaaac gatcgagcgc ccgggtcgcg
     9421 gcgacgagct cgaccctgtc cttggcaaag atcaccttct cgatcagcgc gtcgatgccc
     9481 gcgtccttga tgccgatgat gttgcgcgta ccctcgcgat cggccgcggc cgagccccag
     9541 aactcgcgct gctcattgcc gggtgaaatg gattggccga aggaagcgac gacgatgtcg
     9601 aagtcgaagc tcgagcggcg ctttatgtac tgcgagctgt cgacaatgcg cacgctcgcg
     9661 tggatgccga gcgactcgag ttccttgatg taggggagga tcaggcgctc gaagtcgcgc
     9721 gagacgatca ggaactcggc ggtgagcacc tcgcccgagg ccttcttcag aacgggtttg
     9781 cctgtgctcg ccgggtcgat gcgccagccg gcctcggcga gcaggcgctg cgcctcgcgc
     9841 atgttggcgc gtgcctccgt gaggctcgtg tggtggggcg tccgataggg cgtcgtgaag
     9901 atgtcggccg ggacgagcgg gcggatctcc tcgagaatgg ccttctcgcg gccctcggga
     9961 atgccggttg ccttgagctc ggagccctcg aagaagcttt cgacgcgctt gtaggcgctg
    10021 aacatgatct ttttgttgag gccgtcgaag tcgaaacaga ggtcgaaggc gcgccggacc
    10081 cgccagtcct ggaactgcgg ccgtcgcaga ttgaaggcga acccctgcat aggatgcagg
    10141 ctcgcgagct tgacctcgct ccgcttgacg tggccgcgct tcacggcatc gaagtcgtag
    10201 cccgtggccc agcccttggc gctcggctcg cgccagtagt cgaggttgcc ggccttgaaa
    10261 tcgttgaagg cgacctggcg gtcgtcgaag tagatgaact tgatctcgtc gatgttccac
    10321 tgcccgcgca ttacggggag cttggccgcg aaccagtcct tgacgcgctc gtagacgatg
    10381 tgccggccgg cctcgagcga cttgatgcga taggggccgg agccgagcgg cggctccagc
    10441 gtggccttgg cgatatcgcg ccgtgtgccg tcggcgcctt tcgcctccca atagtggcgc
    10501 ggcaggatcg gcatgtcgcc gacgatatgg gcgagctcgc gattgccttt gacggcgaag
    10561 tggaaggtca cctcgcgctc accgctcgcc tcgaccttgg caacgtcctt atagtagtcg
    10621 atgtacttcg gatcgttgcg cgtgagcgcc tcgaaagtga agaccacgtc gtcaggcgtg
    10681 atcggccgcc cgtcgtggaa ggctgcttcc ggacgcagcc taaacttggc agcggagaaa
    10741 tccttctgca cctcgaccca ctcggcgacg aggccatagc tcgtcgaagg nnnnnnnnnn
    10801 nnnnnngtcc gggctcgtcg acatcagcgt ggcatagagc agggactgcg gcgcggcgag
    10861 gccgaccgct ggcaggccct tgatcgtgaa cgggttcagg gtatcgaagg ttcccggcgc
    10921 gaacatgcgg atggagccgc ccttcggcgc ttccgggttg acccacttga agtgcgtgaa
    10981 ctccggacca taggccggct cgccgatgag cgacatggcg tgcaggcgcc ggcgctgggc
    11041 atcgtcggcg cggctcgacg gcgtggtcgc cgtggtcgcg aagagagcga gtgtgacacc
    11101 cacggcgcat gcaggtcgca agaagccgac gccccgcgcc accttaccag ccttcgcaca
    11161 gtccagcatg ccttcgatcc atcccgcgac acctcggccc ggtgaagatt agcatgttcg
    11221 accgggatgc aacgtggcgt cgagattaaa tcttcgtcag agcagaatgc cgacggccgc
    11281 cccaggggga cggccgcggt ggtccgaacg tccgattgcg tccgcctcaa gcctggggct
    11341 gcggcgtgag cgtaccggga tctggctcct cgcgcggtgg cctcggcttn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnagcgt ctcgtgctcc atgagagccc gcgtgatggc
    11521 ctcaagcttg tccttgtgcg tgtagatgag ttcgcgtgcg gtctggtagc cgacgccgac
    11581 gatgcgccgc acctcctcgt cgatgacctt cgccgtctcc tcgctcatgt tctggcgctg
    11641 ggtgatggca tggccgagga acacctcctg ctggttgtcg gtgtagcgca gcggcccgag
    11701 cttctcgctc atgccccact cggtcaccat gcgcttggcg aggtcggtcg cctgcatgat
    11761 gtcgttggag gcgcccgtgt tcaggtgatc ctcgccgtag atgagctgct cggcgacacg
    11821 tccgccgaaa gtcatggcaa tgcgactctc gcaccacttg cgcgagtagc tgagcttgtc
    11881 gcgctcgggc agcgccatgg tgacgccgag ggagcgaccg cgcgggatga tggtcacctt
    11941 gtgcagcgga tcgctgtgcg gcatcagcac gttgacgagg gcgtggcccg cctcgtggta
    12001 ggcggtcgcg agcttttcct cctcggtcat ggccatcgag cggcgctcgg cgcccatcat
    12061 gaccttgtcc ttggcgtcct cgaactccga ctgcgtgacg atgcgcttgt tgcgacgcgc
    12121 ggcaaggagc gctgcttcgt tcacgaggtt ggctaggtcg gcgccagaaa atccgggcgt
    12181 gccgcgggcg atgaccttgg cgtcgacgtc cggggcgagc ggcaccttgc gcatgtggac
    12241 gcgcaggatc ttctcgcggc cgatgacgtc aggcgccggc actgtgatct ggcgatcgaa
    12301 gcggccgggg cggaggagcg cggggtcgag cacgtcgggc cgattggtcg ccgcgatgat
    12361 gatgatccct tcgttggcct cgaagccgtc catctcgacg agcagctggt tcaatgtctg
    12421 ctcgcgctcg tcgttgccac cgccgaggcc cgcgccgcga tgtcggccga ccgcgtcgat
    12481 ctcgtcgatg aagatgatgc agggcgcgtt cttcttggcc tgctcgaaca tgtcgcggac
    12541 gcggctggcg ccgacgccga cgaacatctc gacgnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    12661 gacgagcagg cagccgcgcg ggatgcgccc gccgagccgc tggaatttct gcgggtcacg
    12721 aaggaactcg acgatctcct ggaggtcgac cttggcctcg tcgacgccgg cgacgtcctc
    12781 gaaggtgacg cggccctgcc gctccgtcag cagcttcgcg cgcgacttgc cgaagcccat
    12841 cgcccggccg ctgccagact gcatctggcg catgaacagg agccagatgc cgatgatgag
    12901 cagcatcggc agccacgaaa tgagcgccga gaggagccac ggcatgtcct cgtccggcgg
    12961 acgggcggtg aagcggatat ccttcttgcg gagcctctcg acgagctggg gatcctcggg
    13021 cgcgaacgtc tggaagcgcg aggcgctgtc acggtagacg ccggagatac gattgccggt
    13081 gatggtgacc tcggcaacct tgtcctgctc gacggcgttg aggaactccg agtagctgac
    13141 gtcgccggcc cgccgcccct gcggctgggt ctggaacatg ttgtacaagg ccgccagcag
    13201 caccaggatg atcacccaga ctgcaaggtt gcggaagtga ggggtcatga tcgcctcatt
    13261 ttgatcgcac gtcggtacga tcggggaacg ccgttgttgc acggcaatag gccatgcccc
    13321 gcggtcttag tcaaaatagg ttgatgcacg ctctttgcca aggcgacagg acgagccatt
    13381 ccgggacctg tgatggaatt ggaggcggcg tcgatagccc aggaagagtg gcaccggtcg
    13441 gaactgccga aatcgcagat tatggcccgc tggcgatcag gtctttttct cgatccgctg
    13501 tgctgctgcc ggtccgccat cggcatcggg cttcggctcg gagagcttgt agaggttgcg
    13561 cagcaacgca tcgagaccgc cgccgggacc attggcgtga aggtcgatgc ggccgacctt
    13621 ctcgacgagc ttctccaggg cctcgagctc ctttagccga agcagcagcg ggttgtcgtc
    13681 catgaggcgg gcggtgttga ggagcgagcg ggtcgcggcc gtctcctcct gacggcggat
    13741 caggttcgct ttggcggtnn nnnnnnnncc tcgacgacct tgttcaggag ctcgcgcacg
    13801 tctcccggca ggatgatgtc cttgacgccg atctcaccca ttgcgcagcc gagctcgccg
    13861 acgcgcggtt gcacataggc gcgcacctcc ttgtcgagcg tgtcgcgggc agcgaggatc
    13921 tcgtcaagcg tgcgggcggc gaccgcttcg cggatggcga attggacgtg cttgtagatc
    13981 gtctggccga gatcgccggt cgccatcatc gccttctcgg cgtcggccac ctgaaagaac
    14041 gccgtcagcg tcacgcggat gccgatgcgg tccttggtca ggatctcctg cgcagtcacc
    14101 tcgagggcct gcgggcgcat gtcgaacttt tgcactcgga tcgttcggcc gacctgccag
    14161 aagacgtgac ggccaggcgc caactgttcc tcgagccggc cgtcgacgat cagcagcgcg
    14221 cgttcgttgg gctcgaccat cgcctcgacg atcagcggcg tccgccagcc ggcgaggcgg
    14281 tcctggtagg gcttcgtcat gcgcagctcg cgggccgcat cgacgagatc gaccgtcacc
    14341 tccgtaaccg acgtccagaa ggctcggatc gagttcggtg tcatgagcgt gctgagctgg
    14401 ccatcaagcg cgatgaggcc gacctgtgtc gggccggtct tcacgatctc gatgttctcc
    14461 tcggcaatgc gggggtgaag ccggctcagc atgagggcgc gctcgatcgg aagctccgtg
    14521 cgcaccacct tgacgatctc gcaggaaagc tcgcggctcg gatcgaaggc cgagaagcgg
    14581 ccaggcggca gcagccgctc gatcttgccg ttgcgcagca gaaagccgcg ctcgtcgtcc
    14641 ttcacggaaa ccttgaacca gaacatcggg aacaactcca gacgcacgtg cgtgtacccc
    14701 aatcgatacg atagctactc aaatcctggc tgctcgctag cacaggtcgc cgggctcact
    14761 ccacgactgt gatacggtcc gccgcttgag cctgcgggac cgcgcacttc cgtccgactg
    14821 cgcatgcagc cgccgtggcg gatgcaccgg gatcggaccg tcctgcacag tcccgcgggc
    14881 ggtatccacg gcggcacaga gcgtcacgtt cgctgtcgcc ttgctctccc agccggggcc
    14941 ggcagaggtc ggcggctgga gttcgcccgg cttcccgcga tggaagaccg gttacaagcc
    15001 gggtgttcgg gtttcaatcg aacggggacg ttggagtcga accaactaca gcaagcgttg
    15061 aagcttgagc tctaccaatg agctagtcca ggagcacctg aaaggaatcg aacctcctac
    15121 ctcgggggcc aacccccgcg ctctaccact gagctacagg cgcatgcccg aagcgcggaa
    15181 cacgccgccg cagggcgggg cgcgccagcc gggcctcagc gagcccggac acatgacgca
    15241 aagggcaagc agacggtagt tgtgcgggtt gccggggtca aggctcgtcg gccggaaggc
    15301 aggcggggcg gatattgcag cgatccgatt gcgccgcggc gggacttgag cgacgcttgc
    15361 gctcacatcg ctcttcaggg gaatgctcat gagcccgcgc cacgactacg acctcttcgt
    15421 cattggcgcc ggctccggcg gcgtgcgcgc ggcgcggatc gcggcgagcc atggcgcgcg
    15481 cgtcgcaatc gccgaggagt atcgcgtcgg cggcacctgc gtgatccgcg gctgcgtgcc
    15541 gaaaaagctc ctcgtctatg cgagccgctt ccacggcgag ttcgaggatg ccaaggcgtt
    15601 cgggtggacg gtctgcggtc cgacggaccc gttgcgatc
//
LOCUS       DAORNH010000073        15458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2912_length_15458_cov_2.930533, whole genome shotgun sequence.
ACCESSION   DAORNH010000073 DAORNH010000000
VERSION     DAORNH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_15458_cov_2.930533"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PK264_09545"
     CDS             <1..989
                     /locus_tag="PK264_09545"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRD76168.1"
                     /translation="MVKDTSYMFVTGPDVVKTVTNETVTAEELGGAKVHTTRSSIADR
                     AYENDVEAILQMRRLMDFLPSSNTSGVPLLPTRDSPDREDRSLDTLIPDNPNKPYDIK
                     ELILKVVDEADFFEIQEAFAKNIVTGFARMEGRTVGIVANQPMVLAGVLDSDASRKAA
                     RFVRFCDCFEIPIVTFVDVPGFLPGTAQEYGGLIKHGAKLLFAYSEATVPKVTVITRK
                     AYGGAYDVMSSKHIRGDMNYAWPSAEIAVMGAKGAAEILYRAELGDPEKIKARIDEYQ
                     KRFANPFVAAERGYIDEVILPRGTRRRICRALNMLREKKVQNPWKKHDNIPL"
     gene            996..1517
                     /locus_tag="PK264_09550"
     CDS             996..1517
                     /locus_tag="PK264_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76169.1"
                     /translation="MLQPPGPLPHRHLAGHETPWKASKQSEDALQRWHFAGGCMPSYV
                     IMPDIALRLAAEAARPAALVRLLAPTLLRSHVLSELYASFRRGEITRREADGRLEGIR
                     RLRIRLLGDRVLQKMAWTIADQLGWADTLQAEYLALARIQADALVTTDALIVKAARGI
                     VALARYEDILIGR"
     gene            1551..1826
                     /locus_tag="PK264_09555"
     CDS             1551..1826
                     /locus_tag="PK264_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76170.1"
                     /translation="MSTPNEPLRRRRFLRLRIVFFLLSLPLAWWAVTRFGGSAAGYLR
                     SYPSLTGPWFWFATFALAFGAWGIWRLVKRKWARAKELAGEARSRQR"
     gene            complement(1874..3334)
                     /locus_tag="PK264_09560"
     CDS             complement(1874..3334)
                     /locus_tag="PK264_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD76171.1"
                     /translation="MAAYRMIALLIVGLLHGAVQGLVSEARAQDRRVALVIGNASYKH
                     APTLATPLRDVADLAKALENIGFRVIAGRNLTKVEMERTVRSFADALAGAEIGLFFYA
                     GHGLQVAGQNYLVPIDARLSTLTAIDFEMVRLDLIQRAMEHQARTNVLFIDACRDNPL
                     ARDLSRAMGTRSGDVGRGLVAAEAGHGTLISFSTQPGNVALDGTGRNSPFAEALVKRI
                     AAPGEDLSTLLIGVRNDVMRATERRQVPWEHSALTERICLSGPCGAAGDAGTARASEI
                     AFWNSVKDSADAAILKTYLDRYPNGDFASLARALIEKANRTRPDPLPASPAPSMASLL
                     APQPAAIATSAIPIVAAEAKRGLDITRRGILIDVAGDQVVAQTAVDADGAVGRVTVTA
                     LSRGFKSTWFIFALVNSSSEDQTFEISADKVRAATGAPLEAIAAVFASVGFQPKRKAD
                     REKDVFSAQLLPGMTVTYVLEILTGHAPRIHVARMP"
     gene            complement(3372..3938)
                     /locus_tag="PK264_09565"
     CDS             complement(3372..3938)
                     /locus_tag="PK264_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRD76172.1"
                     /translation="MSARGDVLLIVQALAFASEHHASQRRKGDAGEPYINHLAEVADL
                     VGLATRGEDPHLVAAALLHDVVEDQGVSVDELQRLFGEEVAAVVGEVSDDKTLPKAER
                     KRLQIELTPKKSERARLLKLADKTSNLRSMRVSPPSGWSLERRREYLAWARSVANGTR
                     GLSPWLEQRFDEAAAALEATLNGTGERR"
     gene            complement(3943..5001)
                     /locus_tag="PK264_09570"
     CDS             complement(3943..5001)
                     /locus_tag="PK264_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HRD76173.1"
                     /translation="MSYIAKPSAHHPALQRNELGLTHRDYEGSMSTLCAGCGHDSITA
                     AIIEACYELSLPAHRIAKISGIGCSSKTPTYFLGKSHGFNSVHGRMPSVTTGANMANR
                     DLVYIGVSGDGDTASIGLGQFAHAVRRKLNMTYIVENNGCYGLTKGQFSATNDKSSPS
                     KKGETNPFDAIDLCQMAIQLGAGFVARSFSGDKKQLIPLIKAAISYKGFAFLDVISPC
                     VTFNNHNESTKSFNYVRAHNLASEKPLDYVEPKEEITVSYDEGTSTSVTLHDGSRLIL
                     QKLDGRHDVRDNDAAISAIRASAKKGEIVTGLLYLNPVEHDLHDVLTTDSRPLNAIPV
                     AELCPGSKALDAINARMR"
     gene            complement(5043..5615)
                     /locus_tag="PK264_09575"
     CDS             complement(5043..5615)
                     /locus_tag="PK264_09575"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD76174.1"
                     /translation="MPTKLAEADQMTVEEFLAFYEQRPDGEKWELIEGAAVMNPSPTQ
                     WHQVIAGNILFALSERKIAEAATWIPLMGIGTHVPNSPHSLPQPDVYVQSRPVVDAAT
                     TDDALAVFEVLSQSNTKADQAWRRRVYASIPNCRHYVTVATKSAEVTCYDRADQWRAR
                     TLKGLDATLQLSAINAAIPLRLIYRWTPIE"
     gene            complement(5636..7456)
                     /locus_tag="PK264_09580"
     CDS             complement(5636..7456)
                     /locus_tag="PK264_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRD76175.1"
                     /translation="MSGINDFVVKFATVNGSGSASANTIFAKTLFRMGIPVSAKNIFP
                     SNIQGLATWYEVRVSEAGYIARRDGIDLMVAMNPQSYTEDVAAVVPGGYLLYDSSMPR
                     VWPRADIQVLGIPIAQICAKRWPDPRQRQLMKNIIYVGALAALLDIDMDVAKGVVSDQ
                     LKGKERLVAANMEALELGRSYARENFTAPLPIRVRHATGAKGKIMMTGNEAAGLGAVY
                     AGASVCAWYPITPSTSLAESFEKHARRLRVKDGKRLYAIVQAEDELAAIGVAIGGGWN
                     GARSFTATSGPGVSLMGEFLGLAYFAEIPVVLYNVQRGGPSTGMPTRTGQPDIVSTAY
                     ASHGDTKHVLLFPANPTECFSMSAAAFDLAERLQTPVMVMSDLDIGMNDWVTDALQWD
                     DSYTHDRGKVLTAETLEGFKGRHGKEWGRYLDVDGDAIPYRTLPGTHPSMGSFFTRGT
                     SHDEYARYTEDGRVHARVLDRIRRKFDTAATLVPKPEIDIRDKAGKTGLIYFGATEPA
                     VREGLDLLARDGIQINALRLKAFPFSQEVKDFCAAHDRILVVEQNRDAQMRQLLIIEA
                     GVPAEKLVATLDYNGMPTTAAFVRDAVLRLVRPKKAAAAE"
     gene            complement(7507..>7896)
                     /locus_tag="PK264_09585"
     CDS             complement(7507..>7896)
                     /locus_tag="PK264_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76176.1"
                     /translation="PRLAAVIAALEKLHTQVAGSDGEWDAGLFNSLVDKAATEFNDDL
                     SGFEVEVSPIDAEAFANHIEVALEYVATLATHGAGTPADTKPATTAVLDEQAFDAEFE
                     EANEAEQAKGDGGPIRKRMQRLIGRNS"
     assembly_gap    7897..7946
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7947..9862)
                     /locus_tag="PK264_09590"
     CDS             complement(<7947..9862)
                     /locus_tag="PK264_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76177.1"
                     /translation="MTARDIADRSGGGSPGGRPRSGMTPTDIKDPSYFHKVVDCQCAC
                     PAHTPVPEYIRLIAQGRYAAAYMLNWESNVFPGILGRVCDRPCEPACRRGRIQTGDEP
                     NAKDPSRKNAEPVAICRLKRVAADYKGEVDAFMPEIPKARNGKRVALVGGGPASLTVA
                     RDLAPLGYHVTLIEKDPKAGGLMRTNIPSFRLPATVLDEEVDRILGFGVEHRFGVEVK
                     SLKALLGEGFDAVFVGTGAPKGKDLSIPGRKEAAKNIHIGIDWLTSVAFGHAEKVGRR
                     VIVIGGGNTAMDCCRTALRLGAESVTVTVRSPRRVMKASEWELKDAEHEGIPILDNHA
                     PKAFVVEGGRLTGVTFEKQIETGADAKGRPQLKGTGETVTIPCDDVLMAIGQENAFPW
                     IERDIGVEFGEWDMPKVDKLTFMSSRPGVFFGGDAAWGPENIIWAVAHGHQAAISIDA
                     FCQGRPVTERPKPGFTLVSQKMGIHEWSYSNNYDGAHRQDVRHADLAASKRNLKLEVE
                     LGFDDQIAAKEVERCLNCDVQTVFAPKLCIECDACMDICPVDCINFIDNAPEPELRKA
                     LRVPALNASQDLYVSEALPGTGRIMAKDEDVCLHCGLCAERCPTGAWDMQKFFYAETQ
                     AAKACHAHHNAYLR"
     gene            10292..12292
                     /locus_tag="PK264_09595"
     CDS             10292..12292
                     /locus_tag="PK264_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HRD76178.1"
                     /translation="MSNNDHSRDALTRAQQAEEAENKGSNDSPNPTMGDVIAARWTRR
                     DLMHGLLAVSAITVTVSPLALASASRARAQSPNSTPNFNFRELSAGSDEKHHVAEGYD
                     SDILIRWGDPVLPGAPAFDPTRQTAAAQARQFGYNNDYLGFLPLGGKSDHGLLVVNHE
                     YTNEELMFPGLGRQDAAKKFAGMTKELVEIEMMAHGGSVLEVKRVNGRWQVVADSKYA
                     RRITAETEMRVSGPAAGHERMRTNADPTGTKVHGMLNNCAGAMTPWGTWLTCEENFNG
                     YFWGKAAAEAGPDAKQLKRYGAPGEWYAWGRWFDRFDVAKEPNEVNRFGWVVEIDPTD
                     PNSVPVKRTALGRFKHEGAANIVNRDGRFVVYQGDDERFDYVYKFVTEGRVDTANKAA
                     NRDLLDKGTLYVARYNPDGTGVWLPLVHGEGPLTAANGFKSQADVVIWPRLASDLLGA
                     TKMDRPEDVEANPKTNKVYVLLTNNSRRKANEVDPANPRGPNRFGHIIEMLPEGGDHA
                     STKFTWNILVKCGDPAIADVGATFHAATTKDGWFGMPDNCAIDAQGRLWIATDGNAPS
                     KTGRADGLWGVETEGAARATSKLFFRCPNGAELCGPEFTPDLESLFVAVQHPGEADEG
                     DANAAPATFEKPATRWPDFKPDMPPRPSIVAITKRGGGKIAT"
     gene            12381..12707
                     /locus_tag="PK264_09600"
     CDS             12381..12707
                     /locus_tag="PK264_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD76179.1"
                     /translation="MKLKLHHLNLATTDVPGMNSFWRDILGMTTEPTMGQGNLDPNVR
                     SRVEFVTDGGTQFHLTERDLGFGFRAGQAVNPMERGHIAFRTDDIATFKKRLEEKGIP
                     YSDYGP"
     gene            12805..13734
                     /locus_tag="PK264_09605"
     CDS             12805..13734
                     /locus_tag="PK264_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76180.1"
                     /translation="MGLMSVSRRPWIALWAAITLAVPATLQAQEPPASIGLKEVQVTA
                     GGKSARVRYKHYPAPGSTDAIIYLHGDLPNEEMRKAGKDEAFLKYFNLIEKRLQKFSA
                     ERKLQTVMIARPGYIWSSGDARERRTELHYRWIREAIVAIVGELRIERYALAGQSGGS
                     TAAAMVVALGDTATPCVILGSGGYSWIATSRHQNRVFDRRPLTAEEEARGMSRVADPI
                     QRVRDIAVIPARRFVLLADPRDKRTPFAAQVEFWSALRGRGHDAKLVVRAASGAEFHS
                     MSNHAMSAAETCLAETRSQPRWPAPMIDGPAKE"
     gene            13866..14792
                     /locus_tag="PK264_09610"
     CDS             13866..14792
                     /locus_tag="PK264_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76181.1"
                     /translation="MRLYLGLAAAAALLGISSASAAEPPAKAGLEAIAVEANGAKGVV
                     RYKHFPVAGKREAIIYLHGDLPPKAANGDSTDEDLRILQSIERRLRSFSADRLVPTVM
                     IARPGYLWSSGDPEERHSKTHNKWIRDAIEGVVTRLGIERYALVGQSGGSTAIALTLP
                     IGERQSPCVVLGSGSFSWIKTARHQSRMFNMRPITRAIERQIESRDPAPIGTVTEIPV
                     VEGRRIIVLGDPRDWRTPFAAQVEYWAAMRDRGHEAIFMVKEAFDKERHGMSGEAMTA
                     GERCLNETRPDEAPAAVTPAPANHASGARPHA"
     gene            complement(14817..15446)
                     /locus_tag="PK264_09615"
     CDS             complement(14817..15446)
                     /locus_tag="PK264_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRD76182.1"
                     /translation="MPDQAVLLLAPPLVPEVRLHLAEESLAIWQKTEEELGELNVPPP
                     FWAFAWAGGQALARYVLDHPEVARGCATLDLGAGSGLAAIAAMKAGARHALSADPDAF
                     SAAAAAVNAAANGVALETTTADLLDTPPGAFGLVLVGDLFYERTLAERVLAFVTAASA
                     AGATVLVGDPMRSYFPRHRFVPVAEYNVPVTRELEDAEIKRAAVWRLKP"
BASE COUNT         2629 a         4985 c         5154 g         2640 t
ORIGIN      
        1 tcatggtgaa ggacacgagt tacatgttcg tgaccggccc cgatgtcgtg aagacggtga
       61 cgaacgagac ggtgactgcg gaagaactcg gcggcgccaa ggtgcacacg acccgctcct
      121 cgatcgccga tcgcgcctac gagaacgacg tcgaggcgat cctgcagatg cggcgcctca
      181 tggatttcct gccttcgagc aacacctcgg gcgtgccgct tctgccgacg cgcgacagcc
      241 ccgatcgcga ggaccgctcg ctcgacacgc tgatcccgga caacccgaac aagccctacg
      301 acatcaagga gctcatcctt aaggtcgtgg acgaggccga cttcttcgag atccaggagg
      361 cgttcgcgaa gaacatcgtc acgggctttg cgcgcatgga agggcgcacg gtcggcatcg
      421 tcgccaatca gccgatggtg ctcgcgggcg tgctcgacag cgacgcctcg aggaaggccg
      481 cccgcttcgt gcgcttctgc gactgcttcg agatcccgat cgtcacattc gtcgacgtgc
      541 cgggcttcct tcccggcacc gcgcaggaat acggcggcct catcaagcac ggcgccaagc
      601 tgctgttcgc ctactcggag gcgacggtgc cgaaggtgac cgtgatcaca cgcaaggcct
      661 acggcggcgc ctatgacgtg atgagttcga agcacatccg cggcgacatg aactacgcct
      721 ggccctcagc cgagatcgcg gtgatggggg ccaagggcgc ggccgagatc ctctaccgcg
      781 ccgagctcgg cgatccggaa aagatcaagg cgcggatcga tgaataccag aagcgcttcg
      841 cgaacccctt cgtcgcggcc gagcgcggct acatcgacga ggtgatcctg ccccgcggta
      901 cccgccgccg catctgccgc gccctcaaca tgctgcgcga gaagaaggtg cagaacccat
      961 ggaaaaagca cgataatata ccgttgtgag ggcccttgct gcaaccgccg gggcctctgc
     1021 cgcaccgtca cctggcgggg cacgaaacgc cctggaaggc gtcgaagcag agcgaggatg
     1081 ctctgcagcg atggcacttc gccggaggat gcatgccaag ctatgtgatc atgcccgata
     1141 ttgcgctgcg gcttgcagcc gaagctgccc ggccggccgc attggtgcgc cttctcgcgc
     1201 cgacgctgct gcgctcgcat gtgctgtccg aactctacgc gagcttccga cggggcgaaa
     1261 tcacgcggcg tgaggccgac gggcgcctcg aaggcataag gcgcctgaga atacgcctgc
     1321 tcggcgaccg ggtactccag aaaatggcgt ggaccatcgc cgatcagctc ggctgggctg
     1381 atacgttgca ggccgaatac ctggcgctgg ccaggattca agccgatgcc ctcgtgacaa
     1441 cggacgccct cattgtcaag gcggcgcgtg gcatcgttgc attggcgcgc tatgaggaca
     1501 ttctgatcgg ccgctgaggg cgccgccggg agatgctgac gaggaacgcc atgtccacgc
     1561 cgaacgagcc actgcggcgg cgccggttcc tccggctcag gatcgtcttc ttcctgctga
     1621 gcctgccact cgcgtggtgg gcggtgacgc ggttcggcgg cagcgctgcc ggctacctca
     1681 gatcatatcc gagcctcacg ggaccctggt tctggttcgc gacgttcgcc cttgccttcg
     1741 gcgcctgggg catctggcgc ctcgtcaagc gcaagtgggc ccgcgcaaag gaactcgccg
     1801 gagaggcccg atccaggcaa cgttgagctg tgaggcatcg tcgaagccag cggtcgtcgc
     1861 gctgccgcca tgatcagggc atgcgggcaa catggatgcg cggcgcatgt cctgtcagga
     1921 tctcgagcac gtacgtgacg gtcataccgg gcaacagctg cgcactgaaa acgtccttct
     1981 ccctgtcggc cttccgcttc ggctggaatc cgactgaggc gaacaccgca gcgatggcct
     2041 ccagcggcgc tccggttgct gccctcacct tgtcagcgct gatctcgaac gtctgatcct
     2101 cactgctcga attcacgagc gcaaaaatga accacgtcga cttgaagccg cgcgagagag
     2161 ccgtcaccgt gacccgcccg acagcgccat cggcatctac ggccgtttgg gcaacgactt
     2221 ggtcgccggc gacgtcgatg agaatgccgc gccgcgtgat gtcgaggccg cgtttggcct
     2281 cggcggctac gatcggaatg gcggacgtcg cgatagcggc cggttgcggc gccagcagcg
     2341 atgccatcga cggggctggt gacgcgggga gcggatcggg gcgcgtgcga ttggccttct
     2401 cgatgagcgc ccgcgcaaga ctggcgaagt cgccgttagg atagcggtcg aggtaggtct
     2461 tcaggatcgc ggcatcggca ctgtccttca cgctgttcca gaatgcgatt tcgctcgcgc
     2521 gcgccgtgcc agcatcgccc gctgccccgc aaggccccga cagacaaatg cgctctgtga
     2581 gcgcgctgtg ctcccagggg acctgccgac gctcggttgc ccgcatgaca tcgttgcgca
     2641 cgccgatcaa cagggttgag aggtcctcgc ccggcgcggc aatccgcttc accagggcct
     2701 cagcaaaggg cgagttccgc cccgtgccgt cgagtgcaac gttgcccggt tgggtcgaga
     2761 acgagatgag tgtgccgtgt ccagcttcgg cagctacgag cccgcggccg acatcgcctg
     2821 atcgcgtgcc catggccctg gagaggtcac gcgccagcgg attgtcgcgg cacgcatcga
     2881 taaagagcac gttggtgcgt gcctggtgct ccatcgctcg ctggatgaga tcgagccgga
     2941 ccatctcgaa atcgattgcc gtcaacgtcg agagtcgggc gtctatcgga acgagatagt
     3001 tctggccagc gacctggagt ccgtgaccgg cgtagaagaa cagcccgatt tccgcgcccg
     3061 cgagtgcgtc ggcgaaggac cggaccgtcc gctccatctc gacttttgtc aggttccgtc
     3121 ccgctatgac gcgaaagccg atattctcga gtgccttggc aaggtccgcg acgtcgcgca
     3181 gcggtgtcgc gagtgtcggc gcgtgcttgt agctcgcgtt gccgatgacg agtgcgacgc
     3241 gcctgtcctg cgcgcgagct tcactgacca agccttgcac tgcgccgtgc agtaggccga
     3301 caatgaggag ggcgatcata cgatatgcag ccatgatcct cctcgcatat cgcgatctga
     3361 caaacttgat cctaccgtcg ctcgccagtc ccgttcagcg tggcctcgag tgccgctgcg
     3421 gcctcgtcga agcgctgctc gagccagggc gagagaccgc gcgttccgtt ggcgacagag
     3481 cgcgcccagg cgaggtactc gcggcggcgc tcgagcgacc agcccgaggg tggcgacacg
     3541 cgcatggagc gcaagttcga cgtcttgtcg gcgagcttca ggagccgcgc ccgctcgctc
     3601 ttcttcggcg tcagctcgat ctgcaggcgc ttcctctcgg ccttcggcag tgtcttgtcg
     3661 tccgagactt cgccgaccac ggccgcgacc tcctcgccga agagacgctg aagctcatcg
     3721 acgctgacgc cctgatcctc gacgacgtcg tgcaagaggg ctgccgcgac gaggtgcggg
     3781 tcttctccac gcgtggccag cccgacgaga tcggcgacct cggccagatg attgatgtag
     3841 ggctcgccgg catcgccctt gcgacgctga ctcgcatgat gctccgaggc aaacgccagg
     3901 gcctgcacaa tcagaagcac atcgccgcgc gcgctcaacg gcttacctca tccgcgcgtt
     3961 gatcgcgtcg agtgccttgg agcccgggca gagctcggcg accgggatcg catttagcgg
     4021 gcggctgtcc gtcgtcagca cgtcgtggag gtcgtgctcg accggattga gatagaggag
     4081 gcccgtgacg atctcgccct tcttggcact ggcacggatt gccgagatgg ctgcgtcgtt
     4141 gtcgcggaca tcatggcggc cgtccaactt ctggaggatc aggcgcgagc cgtcgtgcag
     4201 cgtgaccgag gtcgaggtgc cttcgtcata gctcaccgtg atctcctcct tgggctcgac
     4261 atagtcgagc ggcttctcgg aggcgagatt gtgggcgcgg acgtagttga agctcttggt
     4321 cgattcgttg tggttgttaa aggtgacgca cgggctgatc acgtccagaa aggcgaagcc
     4381 cttgtaggag atcgcggcct tgatgagcgg gatcagctgc ttcttgtcgc ccgagaacga
     4441 gcgggcgacg aagccggcgc cgagctggat cgccatctgg cagaggtcga tcgcgtcgaa
     4501 cgggttcgtc tcgcctttct tggagggcga ggacttgtcg ttggtcgcgg agaactggcc
     4561 cttggtgagg ccatagcagc cgttgttctc gacgatgtag gtcatgttga gcttgcggcg
     4621 gacggcgtgg gcgaactggc cgaggccgat cgatgccgta tcgccatcgc cggagacgcc
     4681 gatgtagacg aggtcgcggt ttgccatgtt ggcgccggtc gtcaccgacg gcatgcggcc
     4741 gtgcaccgaa ttgaagccgt ggctcttgcc gaggaagtag gtcggcgtct tcgacgagca
     4801 gccgatgccg gagatcttgg cgatacggtg ggcgggcaga gagagctcgt agcaggcctc
     4861 gatgatggcg gccgtgatcg aatcgtgccc gcagcctgcg cagagcgtcg acatcgagcc
     4921 ttcgtagtcg cgatgcgtga ggccgagttc gttgcgctgc aacgccggat ggtgggcgga
     4981 cggctttgcg atgtaggaca tgaacggcgg tctccaggcg gtatcgggtg tgttgtgcgg
     5041 gcttattcga tcggcgtcca gcggtagatc agcctgagcg ggatagcggc gttgatcgcg
     5101 ctcagctgca gtgtcgcatc aagccccttg agggttcggg cacgccattg gtcggcgcgg
     5161 tcgtagcagg tgacctcggc ggacttggtc gccacggtca cgtagtgccg gcagttgggg
     5221 atgctcgcat agacgcggcg gcgccaggcc tggtcggcct ttgtgttcga ttgggaaagg
     5281 acctcgaaga cggccaaggc atcgtccgtc gtcgccgcat ccacgaccgg tcgcgactgc
     5341 acgtagacat ccggttgagg cagactatgt ggcgagttcg gcacgtgcgt gccaataccc
     5401 atgaggggga tccaagttgc ggcttcggca atcttccgtt cactcagggc gaacagaatg
     5461 ttgcctgcga tgacctgatg ccactgtgtc ggcgatgggt tcatgactgc cgcaccttcg
     5521 atcagctccc acttttcgcc atcggggcgc tgttcgtaga aggcgaggaa ctcctcgacc
     5581 gtcatctgat cggcttcggc gagcttggtt ggcatgggcg gctccttcgc tcacctcact
     5641 cggcggccgc ggccttcttg gggcgcacga gacggagcac cgcatcacgc acgaacgccg
     5701 ccgtcgtcgg catgccgttg tagtcgaggg tcgcgacgag tttctctgcc ggcacgcccg
     5761 cctcgatgat gagcagctgg cgcatctgcg catcgcggtt ctgctcgacg acgaggatgc
     5821 ggtcgtgcgc ggcacagaag tccttcacct cctggctgaa cgggaaggcc ttcaaacgca
     5881 gcgcgttgat ctggatgccg tcccgggcga ggagatcaag gccctcgcgc acggcgggct
     5941 cggtcgcgcc gaagtagatg agtccggtct tgccggcctt gtcgcggata tcgatctcgg
     6001 gcttcggcac gagcgttgcc gcggtgtcga acttccggcg gatgcggtcg aggacgcggg
     6061 catgaacgcg tccgtcctcg gtgtagcggg cgtactcgtc gtggctcgtg ccgcgggtga
     6121 agaacgagcc catgctcgga tgtgtgccgg ggagcgtgcg atagggaatc gcgtcgccgt
     6181 cgacgtcgag atagcggccc cactccttgc cgtgccggcc cttgaagccc tcgagtgtct
     6241 cggcagtcag caccttgcca cggtcgtggg tgtagctatc gtcccactga agcgcatcgg
     6301 tgacccagtc attcatgccg atgtcgaggt ccgacatcac catgacaggt gtctgcaggc
     6361 gctcggcaag atcgaacgcg gcggcactca tcgagaagca ttctgtcgga ttggcgggga
     6421 acagaagcac gtgcttggtg tcgccgtggc tcgcataggc cgtcgagacg atgtcgggct
     6481 ggccggtccg cgtcggcatg ccggtcgatg ggccgccccg ctgcacgtta tagagcacga
     6541 ccgggatctc ggcgaagtag gcgagcccca aaaactcgcc catgagcgag acgcccggcc
     6601 ccgaggtcgc agtgaatgag cgcgcaccgt tccagccgcc gccgatggca acgccgatgg
     6661 ccgcgagctc gtcctcggcc tgaacgatcg cgtagaggcg cttgccgtcc ttcacgcgga
     6721 ggcggcgcgc gtgcttctcg aaggactcgg cgagcgaggt cgacggcgtg atggggtacc
     6781 aggcgcagac cgaggcacct gcgtagaccg cgccaaggcc tgccgcctcg ttgccggtca
     6841 tcatgatctt gcccttggcg cccgtggcgt ggcggacgcg gatgggcagg ggcgcggtaa
     6901 aattctcgcg agcgtaggag cggccgagct cgagcgcctc catattggcg gcaactaagc
     6961 gctccttgcc cttcagctga tcgctgacga cgcccttggc gacgtccata tcgatgtcga
     7021 gcagcgctgc cagcgcgccg acgtagatga tgttcttcat cagctggcgc tggcgcgggt
     7081 ccggccatct ctttgcgcag atttgcgcaa tcgggatgcc gagcacctgg atgtcggcgc
     7141 ggggccacac gcggggcatg ctcgagtcgt agagcaggta gccgccgggc acgactgccg
     7201 ccacgtcctc cgtgtaactc tgcgggttca tggcgaccat gaggtcgatg ccgtcgcgcc
     7261 gggcgatata gccggcttcc gagacgcgca cctcatacca ggtggcaagg ccctggatat
     7321 tcgacggaaa gatgttcttc gccgagacgg ggatgcccat ccggaacagg gttttcgcga
     7381 aaatcgtgtt ggcagaggcg gagcccgagc cgttgactgt cgcgaacttg acgacgaaat
     7441 cgttgatgcc gctcatcgta ccttgagacc tcggggtttg ctcactgccc gcattggggt
     7501 gcttcgtcac gaattgcgcc cgatgaggcg ctgcatgcgt ttgcggatcg gaccgccgtc
     7561 ccctttggcc tgctccgcct cgttggcttc ctcgaactcg gcgtcgaagg cctgctcgtc
     7621 gaggacggct gtcgtggcag gcttggtgtc ggccggcgtg cctgcgccgt gggtcgcgag
     7681 cgtggcgacg tattcgagcg ccacctcgat gtgattggcg aacgcttcag cgtctatcgg
     7741 actcacctca acctcgaagc cagagaggtc gtcattgaac tccgtcgcgg ccttgtcgac
     7801 cagcgagttg aagaggccgg catcccattc gccgtcgcta cctgcgacct gcgtgtggag
     7861 cttctccagg gccgcgatta cggcagcaag gcgaggnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnncacc tcaggtaggc attgtgatgg gcgtggcacg
     7981 cttttgcggc ctgcgtctcg gcatagaaga acttctgcat gtcccaggcg ccggtcgggc
     8041 agcgttcggc gcagaggccg cagtgcaggc agacatcctc gtcctttgcc atgatgcgcc
     8101 cggtgccggg gagggcctcg gagacgtaga gatcctggct tgcattcagt gccggcacgc
     8161 ggagtgcctt acgcagctcc ggctccggcg cattgtcgat gaaattgatg caatcgactg
     8221 ggcagatgtc catgcaggca tcgcattcga tgcagagctt cggcgcgaac accgtctgca
     8281 cgtcgcagtt gaggcagcgc tcgacctcct tggcggcgat ctggtcgtcg aagccgagct
     8341 cgacctccaa cttgaggttg cgctttgagg cggcgaggtc ggcgtgacgc acatcctggc
     8401 gatgggcgcc gtcgtaattg ttcgaatacg accattcgtg aatgcccatc ttctgcgaga
     8461 cgagcgtgaa gccgggcttc ggacgctcgg tcacggggcg gccctggcag aaggcatcga
     8521 tggagatcgc ggcctggtgg ccgtgggcga cggcccagat gatgttctcg ggcccccagg
     8581 cggcatcgcc gccgaagaag acacccggcc tgctcgacat gaaggtcaac ttgtcgacct
     8641 ttggcatgtc ccactcgccg aactcgacgc cgatgtcgcg ctcgatccag gggaaggcat
     8701 tctcctgacc gattgccatc agtacgtcgt cgcaggggat ggtaaccgtc tcgccagtgc
     8761 ccttgagctg cgggcggccc ttggcatcgg cgccggtctc gatctgcttc tcgaacgtga
     8821 cgccagtcag tcggccaccc tcgacgacga aggccttcgg cgcgtgattg tcgaggatcg
     8881 ggatgccctc gtgctcggcg tccttcagct cccactcgga ggccttcatg acgcggcgcg
     8941 gcgagcggac ggtcaccgtg acgctctcgg cgccgaggcg gagcgccgtc cggcaacagt
     9001 ccattgcggt gttgccgccg ccgataacga tgacgcggcg gccgaccttc tcggcgtggc
     9061 cgaatgcgac cgaggtcagc cagtcgatgc cgatgtggat gttcttcgcc gcttccttgc
     9121 ggccggggat cgagagatcc ttgcccttgg gagcgccggt gccgacgaag acggcatcga
     9181 agccctcgcc gagcagtgcc ttcaagctct tgacctcgac gccgaagcga tgctcgacgc
     9241 caaagcccag gatgcggtcc acctcctcgt cgagcaccgt cgccggaagg cggaacgagg
     9301 ggatattcgt gcgcatcagg ccgccggcct tcgggtcctt ctcgatgagc gtgacgtggt
     9361 agccgagcgg ggcgaggtcg cgcgcgacgg tgagcgaggc cgggccgccg ccgaccaacg
     9421 cgacgcgctt gccgttccgc gccttcggaa tctccggcat gaaggcatcg acctcgccct
     9481 tgtagtcggc ggcgacacgc ttcaggcggc agatggcgac tggttcggcg ttcttgcggc
     9541 tcgggtcttt cgcattgggc tcgtcgcctg tctggatgcg tccgcggcgg caggccggtt
     9601 cgcatgggcg atcgcagacg cggccgagaa tgcccgggaa gacgttgctc tcccagttga
     9661 gcatgtaggc tgccgcgtag cggccctgag cgatcaggcg gatgtactcc ggcaccggcg
     9721 tgtgggccgg gcaggcacat tggcaatcca cgaccttgtg gaagtagctc ggatccttga
     9781 tgtccgtcgg ggtcatgccc gatcggggcc gcccgcccgg cgagccgccg ccactgcgat
     9841 ctgcgatgtc acgcgctgtc atttcgcctc gtctccgccc gggtgatcca ctgcatctca
     9901 gtgcagtatt ggcccggccg ccatccctag acacgacata cctagaccac actaggttgc
     9961 cgccacatcg atcgtcgccg tcgaggttgc gatcgatgcc cgcgcttgcg tggacgcacg
    10021 tctcgcgtga cccgccactc gcttcgggcg tcgtgccact tctatagagg ttggccgttt
    10081 cctcgccaag tccggctttg gatataccgc ttgcgagttg tgaaatgacg acacggccgt
    10141 gccctcgctg cgtagcgcgg ttcccggccc gggcacggcc gcggccgatg gtgggccatc
    10201 gaccgcaccg tcatatgcgg ttcacgtccc agcgttaacg agccatcaag accacgtcgt
    10261 cgatctgaag tctaccagca cgaggactgc aatgtcgaac aatgatcatt cacgcgatgc
    10321 actcacgcgg gcgcaacaag cagaagaggc cgagaacaag ggcagcaacg acagccccaa
    10381 cccgaccatg ggcgatgtga tcgcggcccg ctggacccgg cgcgatctga tgcacggttt
    10441 gctcgccgtc tcggccatca cagtgaccgt gtcgcccctc gcccttgcga gcgcgagccg
    10501 cgcgcgtgcg caatcgccga acagcacgcc gaacttcaat ttccgcgagt tgagcgcagg
    10561 ctcggacgag aagcaccatg tcgccgaggg ctatgacagc gacatcctga tccgctgggg
    10621 cgaccccgtg ctgccaggtg cgcctgcgtt cgatccgacc cgccagaccg ccgcggccca
    10681 ggcccgccag ttcggctaca acaacgacta tctcggtttc ctgccgctcg gcggcaagag
    10741 cgaccatggc ctgctcgtcg tcaatcacga gtacacgaac gaggagctga tgttccctgg
    10801 cctcggacgc caggacgccg ccaagaagtt cgccggcatg acgaaggaac tcgtcgagat
    10861 cgagatgatg gcccacggcg gctccgtcct cgaggtgaag cgcgtcaatg gccgctggca
    10921 agtcgtcgcc gacagcaagt acgcgcgccg gatcacggcc gagaccgaga tgagggtttc
    10981 cggccccgcc gcgggacacg agcgcatgcg caccaacgcc gatccgaccg gcaccaaggt
    11041 gcacggcatg ctcaacaact gtgccggcgc catgaccccc tggggcacct ggcttacctg
    11101 cgaggagaac ttcaacggct acttctgggg caaggccgcg gccgaggccg gccccgacgc
    11161 caagcaactc aagcgctacg gcgctcccgg cgagtggtat gcctggggcc gctggttcga
    11221 tcgcttcgat gttgcgaagg agccgaacga ggtcaatcgc ttcggctggg tcgtggagat
    11281 cgacccgacc gaccccaact cggtgccggt gaagcgcacg gccctcggcc gcttcaagca
    11341 cgagggcgcc gccaacatcg tcaaccgcga cggccgtttc gtcgtctatc agggtgacga
    11401 cgagcgcttc gactacgtct acaagttcgt gaccgagggc cgcgtcgaca ccgccaacaa
    11461 ggctgccaac cgcgacctcc tcgacaaggg cacgctctac gtcgcacgct acaacccgga
    11521 tggaacgggc gtgtggctgc cgctcgtcca cggtgagggg ccgctcacgg cggcgaacgg
    11581 cttcaagtcg caggccgacg tggtcatctg gccgcgcctt gcctccgacc tcctcggcgc
    11641 gaccaagatg gaccggccgg aggatgtcga ggcgaacccg aagaccaaca aggtctatgt
    11701 gctgctgacc aacaactcgc ggcgcaaggc gaacgaagtc gacccggcca acccgcgcgg
    11761 cccgaaccgc ttcggtcaca tcatcgagat gctgcccgag ggcggcgacc acgcctcgac
    11821 caagttcacg tggaacatcc tcgtgaagtg cggcgatccc gcgatcgccg acgtcggcgc
    11881 gaccttccac gccgcgacga ccaaggacgg ctggttcggc atgccggaca attgcgcgat
    11941 cgatgcacag ggccgcctct ggattgcgac cgacggcaac gcaccaagta agaccggtcg
    12001 cgccgatggc ttgtggggcg tagagaccga gggggccgcg cgcgcgacct cgaagctctt
    12061 cttccgctgc ccgaacgggg cagagttgtg cgggccggag ttcacgcccg acctcgaatc
    12121 gctgttcgtt gcggtgcaac atcccggcga agccgacgag ggggacgcca acgcggcgcc
    12181 tgccaccttc gagaaaccgg cgacgcgctg gcccgacttc aagccggaca tgccaccgcg
    12241 tccgtcgatc gttgccatca ccaagcgcgg cggcggcaag atcgcgacct gatgcgacgc
    12301 acgagccgat gaggccagtg gaattttccc gggcaagccg ctaggcttgc ccgagagcgt
    12361 ttcaggcaag cggacgtcca atgaagctca agctgcacca cctcaatctc gccaccaccg
    12421 acgtgccggg catgaacagc ttctggcgag acatcctcgg catgaccacc gaaccgacca
    12481 tggggcaggg caacctcgac cccaatgtcc gctcgcgcgt cgagttcgta accgacgggg
    12541 gtacgcagtt ccacctgacc gagcgcgatc tcggcttcgg cttccgggcc ggccaggcgg
    12601 tgaacccgat ggagcgcgga cacatcgcgt tccgcacgga cgacatcgcc accttcaaga
    12661 agcggctcga ggagaagggc atcccctatt ccgactatgg tccgtgaagg gctggcagca
    12721 gatattcttc tatgatccgg caggccatat catcgaggtg caccaggcac ctgaataagt
    12781 ggcgggcagt gaggagacgg gctgttgggc ttgatgagcg tcagtcgcag accatggatc
    12841 gcgctctggg cggccatcac ccttgccgtg ccggccacgc ttcaggccca ggagccgccg
    12901 gccagcatcg ggctcaagga ggtccaggtc acggccggcg gtaagtcggc acgggtccgg
    12961 tacaagcact atccggcgcc cggctccacg gatgcgatca tctacctgca cggcgatctt
    13021 cccaacgagg agatgcgcaa ggccggcaag gacgaggcat tcctcaagta cttcaacctg
    13081 atcgagaagc ggctgcagaa gttctcggcc gagcgcaagt tgcagaccgt catgatcgct
    13141 cggcccggct acatctggtc gagcggagat gcccgcgagc ggcgcaccga gctccactat
    13201 cggtggattc gcgaagcgat cgtcgccatc gtcggcgagc tgcggatcga gcgctatgca
    13261 ctcgccgggc aatcgggtgg gtcgacggca gcggccatgg tcgtcgccct cggcgatacg
    13321 gcgacgccgt gcgtcatcct cggctcaggt ggctactcgt ggatcgcgac gagccggcat
    13381 cagaaccgcg ttttcgatcg ccgccccttg acggcggagg aagaggcgag gggcatgtcg
    13441 cgcgtcgccg atcccatcca gcgggtgagg gacattgcgg tcatcccggc gcggcgtttc
    13501 gtgctactgg ccgacccgcg cgacaagcgc accccgttcg cggcccaggt cgagttctgg
    13561 tctgcgctcc gcgggcgcgg ccatgatgcc aagctcgtcg tgcgcgcggc gtcaggcgcg
    13621 gaattccact cgatgtcgaa ccacgcgatg tcggcggccg agacctgcct cgcggagacg
    13681 cggtcgcaac cgaggtggcc ggcgccgatg atcgacgggc ccgccaaaga ataatagcac
    13741 tcccgacgtc gcgcctgaac gtcccgtgaa caatcagcag ggccgccgcc gattcgcact
    13801 ctcgcaggca caactgcctc ccaaaggcct gcaacggccg ttgtctcgcc ctgggaggcc
    13861 tcaccatgcg tctctatctc ggattggccg ccgccgccgc tctcctcggg atcagttccg
    13921 cgagtgctgc cgagccgcct gccaaggccg ggcttgaggc gatcgcggtc gaggccaacg
    13981 gcgccaaggg cgtagtccgc tacaagcatt tcccggtcgc cggcaagcgc gaggcgatca
    14041 tctatctgca cggcgacttg ccgccgaaag cagccaacgg cgattcgacg gatgaggacc
    14101 tgcgcatcct gcagtcgatc gagcgccgtc tgagatcctt cagcgccgac cgactcgtgc
    14161 cgaccgtcat gatcgcgcgc ccgggctacc tctggtcgag cggcgacccg gaagagcgcc
    14221 attcgaagac ccacaacaag tggatccgcg atgccatcga gggtgtcgtg acgcgcctcg
    14281 gcatcgagcg ctatgcgctt gtcggccagt ccggcggatc gacggcgatc gcgctcacct
    14341 tgccgatcgg cgagcggcag agtccgtgcg tcgtgctcgg gtcggggagc ttctcctgga
    14401 tcaagacggc gcggcaccag tcgcggatgt tcaacatgcg gccgatcacg cgggcgatcg
    14461 agcgccagat cgagtcgcgt gacccggcgc caatcgggac ggtcacggag atccccgtcg
    14521 tcgagggccg tcgcatcatc gtgctcggcg acccgcgcga ttggcggaca ccattcgcgg
    14581 cccaggtcga gtactgggcg gccatgcgcg atcgcggcca cgaggcgatc ttcatggtca
    14641 aggaggcctt cgacaaggag cgtcacggca tgtcgggcga ggccatgacc gccggcgagc
    14701 gctgcctcaa cgagacgcgg ccggacgagg cgccggcagc cgtgacaccc gcgcccgcca
    14761 accatgcttc gggtgcgcgg ccgcacgctt gaggcgcttg ccgatcgggt tcgaaatcaa
    14821 ggcttcaggc gccacaccgc cgcgcgcttg atctcggcgt cctcgagctc gcgcgtgacg
    14881 ggcacgttgt actccgcgac gggcacgaac cggtggcggg ggaagtagct ccgcatcgga
    14941 tcaccgacga gcacggtggc gccggcagcg cttgctgccg tgacgaaggc gagcacccgt
    15001 tcagccagag tgcgctcgta gaagagatcg ccgacgagca cgagaccgaa cgccccgggc
    15061 ggcgtgtcga ggaggtcggc cgtggtcgtc tcgagggcga cgccgttggc cgcggcattg
    15121 acggcagccg cggctgcgga gaaggcatcc ggatcggccg aaagcgcatg ccgggcgccc
    15181 gccttcatcg cggcgattgc ggcgagcccc gatcccgcgc cgagatcgag cgtcgcgcag
    15241 ccgcgcgcca cttcggggtg gtcgagaacg tagcgcgcca gcgcctgacc gccggcccag
    15301 gcaaaggccc agaacggcgg cgggacgttc agctcgccga gctcctcctc cgtcttctgc
    15361 cagatggcga ggctctcctc ggcaaggtgg agccggacct ccggcacgag cgggggcgcg
    15421 agcagcagca ccgcctggtc gggcatcgag aacgccgc
//
LOCUS       DAORNH010000074        15414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2923_length_15414_cov_2.532912, whole genome shotgun sequence.
ACCESSION   DAORNH010000074 DAORNH010000000
VERSION     DAORNH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_15414_cov_2.532912"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..372
                     /locus_tag="PK264_09620"
     CDS             94..372
                     /locus_tag="PK264_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76183.1"
                     /translation="MRLVDALLSSLGAIRAHALRSVLTMLDARGGGSGAAIGMHEDGQ
                     SDYSSGSPGSSGSSFGATSSSPPRRSPGAGSGSSSRPVDKKLDDEIPF"
     assembly_gap    508..532
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            644..1999
                     /locus_tag="PK264_09625"
     CDS             644..1999
                     /locus_tag="PK264_09625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD76184.1"
                     /translation="MKREMTLVGFLQAQNCSNYVGSWRHPSTATDFLTPEYYQRIART
                     LEYGKFHVSFFDDRLAMPDILGNDHAEAVRNGVRVVKMDPMTILTCMGMATERLGLGC
                     TYSTTYYEPFHVARAFSTFDLMVRGRGAWNVVTSLNNSEALNFSQAEHPEHDLRYDKA
                     DEFMEVVLGHWDSWADDAIVADKTSGLFAHAEKVRRLDHKGRWFRSKGPFTVPRSPQG
                     RPVLIQAGQSGRGQKFAGRWGELIFVVFPNLELGKKGYQTYKDTIAGLGRDPASVKLA
                     PAVYCITGETKTMAEDKRALIDSLAKPVDALALLSEVLNFDFASRPMDEPLSDADVKS
                     MSGLRAIIDRVATLSGKTKPTIRDFVQFSNRGTIREFPCFVGTPKDVADQMEQWFTAP
                     ACDGFVIAATHLPGGYEDFVRLVVPELQRRGLHQKDYKGSTLRENLGLPRAEIDDWRA
                     S"
     gene            2364..2654
                     /locus_tag="PK264_09630"
     CDS             2364..2654
                     /locus_tag="PK264_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76185.1"
                     /translation="MPGAAKTGETTSEALGARTKCTLDERLSLSSLPEVITDPAIPRP
                     PMPSEILSYINDHLIELKHLADSLRAVPLAEAIDAAQNEAFRAAVMQKLLKR"
     gene            complement(2770..3915)
                     /gene="dprA"
                     /locus_tag="PK264_09635"
     CDS             complement(2770..3915)
                     /gene="dprA"
                     /locus_tag="PK264_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRD76186.1"
                     /translation="MRAPLPLAPLDDAQRLACLRLALSENVGPVAFRELINLFGGAAP
                     ALEALPRLAGRGGKRSVRVCPRDRAEAQLQAARRVGAEIVFTIEPGYPMALASLEVPP
                     PMLYLKGRKELLSRPAIAIVGSRNCSAAGHSLARQWSEALGRAGLIVVSGLARGIDAA
                     AHAAALSTGTVAVLAGGVDVAYPPENADLQARIAEEGCLIAELPPGYAPRGQDFPRRN
                     RIISGVARAVVIVEAARRSGSLITARLANEQGRQVYAAPGHPLDPRAEGTNQLIKSGA
                     TLVTSAHDILSDLRPSIGLSDEPDGHEVPSGRRHMADRAPSPEPFPDDDAREKVVAAL
                     GPAPVGIDELARATGLAARDLQIVLLELSLAGRIDRQGGGLVSLRAA"
     gene            complement(3923..4579)
                     /gene="plsY"
                     /locus_tag="PK264_09640"
     CDS             complement(3923..4579)
                     /gene="plsY"
                     /locus_tag="PK264_09640"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRD76187.1"
                     /translation="MGSIYLWPLLVTFLIGYVFGSIPFGLILTRAAGLGDVRKIGSGN
                     IGATNVLRTGNKGLAAATLVLDALKGTAAILATLLALRWFLLGGMPSDAARVALAQSA
                     YWAAGLGAIAGHMFPVWLGFRGGKGVATFIGVLIGIYWPAAAAFAVVWLIVAAMTRYS
                     SLSALIATVAVTGLVIATSTSPVPIAASVLMAILIFWKHRENIRRLLNGEESRIGAKA
                     "
     gene            complement(4646..5971)
                     /locus_tag="PK264_09645"
     CDS             complement(4646..5971)
                     /locus_tag="PK264_09645"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRD76188.1"
                     /translation="MVKPVPGTPAATVFINARVIDPASGRDELGGVLVRNGAIADLGT
                     KFRHNGGEGATIVDCLGHVLCPGLIDAQVFTGEPGQEHRETLRTASQAAAAGGVTTII
                     CMPDTAPVIDEVALVDYIQRRGRDNASVNVHIMAAVTKGLRGEEMTEIGLMQRAGAVA
                     FTNGKSSISSTRVMRNVLLYAKDFGALIVHHTEDPWLSEGSAMNSGELSARLGLPGAH
                     RLAETIVLERDVRLVEMTGGRYHAASISTATSLEVLKRAKAQGLPVTAAVSINHLTLN
                     ENDIGPYRTFFKVRPPLRSEEDRRAMVRGIADGDIDLIVSSHDPQDVDVKRRPFAEAA
                     DGAIGLETLLTAALRLVHSDDLSLATLLKSMTINPARLFGLPGGRLEKGAPADLIVVD
                     PGQPWVVDKALIKARSKNSPFDESKMQGRVLRTMVGGHMVYEYASGGRG"
     gene            complement(5980..6933)
                     /locus_tag="PK264_09650"
     CDS             complement(5980..6933)
                     /locus_tag="PK264_09650"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRD76189.1"
                     /translation="MSTLTHLSGFPRRHLLTCEGLSAAEITSLLDLADKAAETNRQIN
                     KKRDVLRGRTLINLFFEASTRTQSSFELAGKRLGADVMNMNVATSSMQKGETLIDTAM
                     TLNAMRPDIIVVRHHAAGAVELLSQKIDCSVINAGDGAHQHPTQALLDALTIRRAKGR
                     IQGLTVAICGDVLHSRVARSNINVLNTLGARVRLIAPSTLLPPQADRLGCEVYTDMSK
                     GLDGCDIIMMLRLQRERMQGGRFVPSTREYFHFFGLDHEKLGRAKADALIMHPGPMNR
                     GVEIASTIADHSRSVIRDQVEMGVAVRMAVLEALARHLPNR"
     gene            complement(7068..8180)
                     /locus_tag="PK264_09655"
     CDS             complement(7068..8180)
                     /locus_tag="PK264_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HRD76190.1"
                     /translation="MKDATEPNEETATARVAVDLRRGRGALSNASGRFERTARESFDD
                     GWGSLEGLAAFKTEVAIEQAKSIISTNDSPDISFDQSINPYRGCEHGCSYCYARPTHA
                     YLGHSPGLDFETRLYAKVNAAEVLERELARPGYLCKTIALGAVTDVYQPIERERRITR
                     SVLEVLSRTNHPVGIVTKSALVLRDIDILAPMAAKGLAKVAISLTTLDRDVARRMEPR
                     AATPGRRVDTIRSLAAAGIPVTVMMAPIVPAINDSEIEAILAEARGAGATDAGYVLLR
                     LPLELKELFREWLSEAFPDRAARVISVLRSMHGGRDYVSAFGTRQRGTGPFAEQIGTR
                     FRLASRRLGLDERSLKLRTDLFERPLSERHGQLRLL"
     gene            8346..9638
                     /gene="pncB"
                     /locus_tag="PK264_09660"
     CDS             8346..9638
                     /gene="pncB"
                     /locus_tag="PK264_09660"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRD76191.1"
                     /translation="MTDIATRVYSHTWKIDPIVRSVLDTDFYKLLMTQTIFRRHRQRR
                     VTFGLINRTQSVPLARLIDEGALREQLDHIRSLSLSRGESTWLRGNTFYGKRQMFSPE
                     FIGWLEEFRFPPYNLERRGDQYELTFEGSWVETTMWEIPALAVIMELRSRAVLKDLGK
                     FELQVLYARGMARVWEKIERLKQIAGLRIADFGTRRRHGFLWQDWCVQAMQEGLPSSF
                     LGTSNCLIALRREVEAIGTNAHELPMVYAAIAETEQQLSASPYQVLADWQQDYDGNLR
                     IILPDTFGSDGFLAHAPDWVSLWTGMRIDSGDPIEGGERAIAWWRARGQDPATKLLIF
                     SDGLDVAEIEAISAHFKDRVRLGFGWGTLLTNDFRSLVAGRQLDPFSLVCKVVSADGR
                     PAIKLSDNPTKAMGPTGEIARYRRVFSAETGRAIPVTV"
     gene            complement(9628..9981)
                     /locus_tag="PK264_09665"
     CDS             complement(9628..9981)
                     /locus_tag="PK264_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HRD76192.1"
                     /translation="MRIPSELWVKAYVRQRSATGLGAFIVKRGDDRGGAIWIKVARLD
                     GTASLYGPAMAGMIEAASERRFEARRVDQPEREIDDLLTAERRFDDDIWIVEIEDRQG
                     RHGLGDDLIEPEIKR"
     gene            10181..>11368
                     /locus_tag="PK264_09670"
     CDS             10181..>11368
                     /locus_tag="PK264_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76193.1"
                     /translation="MGLTRRGLLAVAGSIAAAAISAQAPPVMAQQKVLRVVMHSDLKI
                     VDPIWTTAYITRNHGYMIYDVLFALDAKGEIQPQMVDKYEVSADKLSYTFTLRDGLTW
                     HDGKPVTGEDCIASIKRWGAKDATGQKLMTFVAGFEAVDAKTFRIKLKEPTGLVLVGL
                     SKPSSSVPFMMPKRVADTDPNKQIEDFTGSGPFVFKNDEWKPGDKVVYVKFDKYVPRK
                     EPASGLAGGKIVYVDRVEWRAISDHQQAVNALLAGEIDLIESPPHELYELIKKDSKVA
                     LFSWNTIGNQYTFRPNWLAKPFDNPKIRKALWYAFNQEDFLKAVIGDPEYYKVCKAFF
                     VCGTPMASEKGMDGLLESNFVKARELLKEAGYDGTPIVLMHSTDLQVLTNLAPVAKQL
                     MEKA"
     assembly_gap    11369..11429
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11430..11767
                     /locus_tag="PK264_09675"
     CDS             <11430..11767
                     /locus_tag="PK264_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76194.1"
                     /translation="PSAGGWHAFLTSWVAADIMNPVMAGYFNSACDKALFGWPCDPEM
                     EKLRDAFARETDPAKLKAIAEAVQVRATEMTSHIHLGQWYSAGAHRKSISGWLATPVP
                     VLWNIKRAD"
     gene            11884..12825
                     /locus_tag="PK264_09680"
     CDS             11884..12825
                     /locus_tag="PK264_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76195.1"
                     /translation="MALYILRRLLATIPVMLIVAILVFLMLRLSPADPAAIIAGDAAT
                     AEDVERIRAKLGLDRNMFSQFFIWLGSILTGDFGESFFYKQRVTQLIGDRIGPTLALS
                     TFTIVIAVLVAVPLGVVAAYKQGSWIDRLVMGFSVLGFSVPVFVTGYILIFVFAIWLN
                     LLPVQGYQPISEGVGGFVYKLVLPAVTLSVVYIALIARMTRTSMLEVLSEDYVRTARA
                     KGQIERKVLFRHALRNAAVPIVTIIGLGVALLIGGAVVTESIYTIPGLGNLTVGAVLA
                     RDYPVIQAVILLFSFAYVMINLAVDIIYTLLDPRIRY"
     gene            12822..13748
                     /locus_tag="PK264_09685"
     CDS             12822..13748
                     /locus_tag="PK264_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76196.1"
                     /translation="MTVTSPAPEADAAAPRATTARKLARHPNVVVGSIIVMLIALLGL
                     FAPLLGTTDPVLNNPSFKLKKPGAEATIRNDDGTKSTYVHKLGTDSLGRDVWSRIAYG
                     ARVSLMVGVSVAVLSISIGLVIGLVAGYIRWLDFIIMRIMDGLMAIPAILLAIALVSL
                     FRRGSGLLPVIIAIVVPDIPRVVRLVRSVVLSVREEPYVEAAVISGTPTAKLIVRHIL
                     PNTVSPLIVQGTFIAAGAILVEAVLSFLGIGVSPDTPTWGNIMADGRTYFRIHPHLIL
                     YPGIVLALTVLAVNMLGDGLRDTLDPKMERRV"
     gene            13745..>15414
                     /locus_tag="PK264_09690"
     CDS             13745..>15414
                     /locus_tag="PK264_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76197.1"
                     /translation="MSTTAPGPNTASTTAPILEVTGLSVSLPKGSDRRFAIEDASFSV
                     GRGEIVCVVGESGSGKSVTAQAIMGLLPKGQLAITAGSIRLLGEETAQNSQAEYRALR
                     GTRMAMVFQEPMTALNPVERVGDQIAEVLEIHTPLSAGERRQRVLDIMRAVHLPDPEQ
                     MIDAYPHQLSGGQRQRIMIAAALILDPALLIADEPTTALDVTTQAQILKLVRELQERR
                     GTGVLFITHDFGVVAEIADRVVVMQHGRIVEQGPRDDVLLRPREAYTRMLIASVPSLT
                     PPAREPKTGDISLVTRALTKTYGAKGFFGGGRVVHAAADVNIEVRRGETLGIVGESGS
                     GKSTVARCIARLIDPTSGELRVEGREIARVPESELRPYRRTIQIVFQDPYRSLNPRRT
                     VGASIVEGPINFGQTPDAALQRARELMTLVGLRPDALDRYPHQFSGGQRQRICIARAL
                     AMEPKLLIADEAVSALDVSVQAQVLALLDDVRKRFDLAVLFITHDLRVAAQVCDRIAV
                     MKSGRVVEHGATKDIFARPQHDYTKALFEAAPGRGFGTRGLAAATAALP"
BASE COUNT         2643 a         5232 c         4810 g         2643 t
ORIGIN      
        1 tctgaagggt gattaataaa ctcaattgca cgccgatgtc cagcaggtcc cgcgcgacgc
       61 agcggcggct ctggcagaac cgtcggaggc agcatgaggc ttgttgacgc gctgctttcc
      121 tcgctgggtg ccattcgcgc gcatgccctg cgctcggtgc tgaccatgct cgatgcgcgc
      181 ggcggcggct ccggcgcggc catcggcatg catgaggacg gtcagtcgga ctactcaagc
      241 ggcagcccgg gaagctccgg gagtagcttt ggcgcgacct cgagcagtcc acccaggcgc
      301 tcgcctggag ctggcagcgg tagctcttcc cgccccgtcg acaagaagct cgacgacgag
      361 atcccgttct gacgtcctag cccgatcgtt tccgtcggga ggcggccaga ggcgattggc
      421 cagggtcaac attcaacgac gtagtcttgc ttcgtcgagg caccaagccg cgacatggcc
      481 attgcctggc gaatgccagg cgataccnnn nnnnnnnnnn nnnnnnnnnn nnttgcgtgg
      541 cccctgatgt atgctccaga tcaagcgcga tgtgcggcgg tcgactacgg tctgctgcac
      601 aatccgggcg gatgtcggca gggagcggag cgagatggcc accatgaagc gcgagatgac
      661 gttggtgggg ttcctgcagg cgcagaactg ctcgaactac gtcggctcat ggcgtcatcc
      721 gtcgacggcc actgatttcc tgacgccgga gtactaccag cgcatcgccc gcacgctcga
      781 gtatggcaag ttccacgtat cgttcttcga cgaccgcctc gcgatgcccg acattctcgg
      841 caacgatcat gccgaggccg tgcgcaacgg cgtccgcgtc gtcaagatgg acccgatgac
      901 cattctcacg tgcatgggaa tggcgaccga gcgcctcggc ctcggctgca cctactcgac
      961 aacctactat gagccgttcc acgtggcccg cgccttctcg acgttcgacc tcatggtccg
     1021 tggccgcggc gcctggaacg tcgtcacctc gctcaacaat tccgaggccc tgaacttcag
     1081 tcaggccgag catcccgagc atgatctgcg ctacgacaag gccgacgagt tcatggaagt
     1141 cgtgctcggc cactgggaca gctgggccga cgatgccatc gtcgccgaca agacgagcgg
     1201 gctgttcgcc catgcagaga aggtgcgccg cctcgaccac aaggggcgat ggttccggtc
     1261 caagggcccg ttcacggtgc cgcgctcgcc ccaaggccgc ccggtcctca tccaggccgg
     1321 ccagtcgggt cgcgggcaga aattcgcagg ccggtggggc gagcttatct tcgtcgtctt
     1381 ccccaacctg gagctcggca agaagggcta tcagacctac aaggacacga tcgcggggct
     1441 gggccgtgat ccggcgagcg tgaagctcgc gcctgcggtc tactgcatca cgggcgagac
     1501 caagactatg gctgaggata agcgcgcgct gatcgacagc ctcgccaagc ccgttgacgc
     1561 actcgcgttg ctctcggagg ttctcaactt cgatttcgcc tcgcggccca tggacgagcc
     1621 gctctcggac gccgacgtga agtcaatgag cgggcttcgc gccatcatcg atcgtgtggc
     1681 aacgctctcc ggcaagacga agccgacgat tcgcgacttc gtccagttct cgaaccgagg
     1741 caccatccgc gagttcccgt gcttcgtcgg cacgccgaag gacgtcgccg accagatgga
     1801 gcagtggttc accgcgcccg cctgcgacgg cttcgtgatt gcggccaccc atctgccggg
     1861 cggctacgag gacttcgttc gcctggtcgt acctgagctg cagcgccgtg gcctgcacca
     1921 gaaggactac aaggggagca cgctgcgcga gaacctcggc ctgcccagag cggagatcga
     1981 cgattggcgc gcgagctgac gagcgcacgt cgcaccgtca cgtgtggcgt ctcgtgtggc
     2041 ggctcgtgtg gcggctcgtg tggcggggcg acccaatttg catcgttgcc ctacatctgc
     2101 aaccattgcc acgcacggcc caggtctggg actggatgtg tcggctgaag gcctcggatc
     2161 gccgccttcg gagctgtccg ccggggcgac gagacctgta ggacgcgtcg tgaacgcgct
     2221 cccgcgagtg tcagtgcgtg tgtcagcgac gagcctccga aaccataagg aaggcctcta
     2281 aattgcgcag atagtggttg catctgaagt gctattttcg tagattgacg tcctgaatgc
     2341 cgcttatttg tgggagtcgg cacatgccgg gtgctgccaa gacaggcgaa acgaccagcg
     2401 aagccctggg cgcacgaacc aagtgcacgc tcgacgagcg tctctcgctt tcaagcctgc
     2461 cggaagtcat cacagatcca gcgatcccgc ggccgccgat gcccagcgag atcctctcct
     2521 acatcaacga ccacctcatc gagctcaagc atctcgcaga cagtttgaga gcggtgccgc
     2581 tcgccgaggc gatcgatgcc gctcaaaatg aggcgtttcg ggcggcggtc atgcaaaagc
     2641 ttttgaagcg ctgacggccg gagaccgtgt gtcgcgggcg ccgtccgcaa gccccgtgtc
     2701 cggccaaaca ctcgaacatg aaggacaggg accacgcagg actgggaata cgccggtccg
     2761 gcgctaacac taagcggcgc ggagcgacac gaggccaccg ccctggcggt caattcggcc
     2821 cgccagcgac aactcgagca gaacgatctg caggtcgcgc gccgcgagcc ccgttgcccg
     2881 agcaagctca tcgatgccga ccggtgccgg accgagcgcc gcgaccactt tctctcgcgc
     2941 gtcgtcgtcc gggaatggct ccggtgaggg cgctcggtca gccatatgcc gccgcccgct
     3001 cggcacctcg tgaccgtctg gctcgtcgga cagcccgatg gagggcctga gatcggacag
     3061 gatgtcgtgg gcgctcgtca ccaacgtggc gccggacttg atcaactggt tggtgccttc
     3121 cgcccgcggg tcgagcgggt ggccgggcgc cgcgtagacc tgtcgaccct gctcgttggc
     3181 gagccgtgcc gtgatgagcg accccgaccg tcgagctgcc tcgacgatga cgaccgcgcg
     3241 cgccacgccc gaaatgatgc ggttgcgccg cggaaaatcc tggccgcggg gagcatagcc
     3301 cggtggcagc tcggcaatca ggcaaccctc ctcggcaatc cgcgcctgca ggtccgcatt
     3361 ttcaggtgga taggcgacat ccacgccgcc cgcgagcacg gcgaccgttc ccgtcgaaag
     3421 cgcagctgca tgggcagctg catcgatgcc gcgagcgaga cccgagacga cgatcagtcc
     3481 ggcgcggccg agcgcctctg accattgccg cgccagcgaa tggccggccg ccgagcagtt
     3541 gcgggagccg acaatggcga tggccggtct cgacagcagc tcttttcgac ccttgaggta
     3601 gagcatcggc ggcggcacct cgaggctcgc gagcgccatc ggatagccgg gctcgatcgt
     3661 gaacacgatt tccgcgccga cccgccgcgc cgcctgcagc tgcgcctcgg cgcgatccct
     3721 cggacagacg cggaccgagc gctttccgcc tcggccggca aggcgcggga gagcctcgag
     3781 cgcaggcgcc gctccgccga agaggttgat cagctcgcga aaggcgaccg gtccgacgtt
     3841 ctcgctcaag gcgagacgca gacaggcgag ccgctgcgcg tcgtcgagcg gcgccagcgg
     3901 caacggcgcg cgcatgggac cttcaggcct ttgcaccaat gcggctctcc tcgccattga
     3961 gcagccggcg gatgttctcc ctgtgcttcc agaaaatcag gatcgccatc agaactgacg
     4021 ccgcgatcgg aaccgggctc gtcgacgtcg cgatgacgag cccggtgacc gcgaccgtcg
     4081 cgatcaatgc cgagagcgac gagtatcgtg tcatcgcggc aacgatcagc cagacgaccg
     4141 caaatgccgc cgccgccggc cagtagatgc cgatcagaac gccgatgaag gtcgccaccc
     4201 ccttcccgcc gcgaaacccg agccacaccg gaaacatgtg cccggcgatt gcgccgagcc
     4261 cggcggccca gtaagcgctc tgcgccagtg ctacccttgc cgcgtcactc ggcatgccgc
     4321 cgaggaggaa ccatctgagt gcgaggagcg tcgcgaggat ggcggctgtt cctttcaacg
     4381 catcgaggac caaggtggca gcggccaacc ccttgttgcc ggtcctcaag acattggtcg
     4441 caccgatgtt gccggagccg atcttgcgca cgtcgccgag cccggccgcc cgcgtgagga
     4501 tgagcccgaa cgggatcgag ccgaagacgt acccgatcag gaacgtgacc agaagcggcc
     4561 acaagtagat cgatcccatc gacgcccccg tcccatcgca ttcaccgccg cgccgccttc
     4621 cgcgtcagcc ctgcaggccc agcaatcaac cgcgcccacc cgacgcatac tcatacacca
     4681 tatggccgcc gaccatggtg cgcagaaccc gcccctgcat tttgctctcg tcgaacggcg
     4741 agttcttcga gcgcgccttg atcaacgcct tgtccaccac ccagggttgg ccgggatcga
     4801 cgacaatcag gtccgcgggc gcgcccttct caagtcggcc gccgggcaac ccgaaaagcc
     4861 gcgccggatt gatcgtcatc gatttgagaa gcgtcgcaag gctcaggtcg tcgctatgaa
     4921 ccaggcggag cgccgcggtg agaagggttt cgaggccgat cgcgccatcc gccgcctccg
     4981 cgaacgggcg ccgcttgacg tcgacgtcct gcggatcgtg gctcgagacg atgaggtcga
     5041 tatcgccgtc ggcaataccg cgcaccatgg cccgtcggtc ctcctccgac cgcagcggcg
     5101 gccggacctt gaagaacgtc cgatagggcc cgatgtcgtt ctcgttgagc gtcagatgat
     5161 tgatagatac cgcggccgtg accggaagcc cttgcgcttt tgcacgcttc aggacctcga
     5221 gggaggtcgc cgtgctgatc gaggcggcgt gatagcgccc gcccgtcatc tcgacgaggc
     5281 ggacgtcacg ctcgagcacg atcgtctccg caagacggtg cgctcccggc aggccgagcc
     5341 gagccgacag ctcgcccgag ttcattgccg agccttcgct gagccaggga tcctcggtgt
     5401 ggtgcacgat gagcgcgccg aaatccttgg catatagcag cacgttgcgc atcacccgcg
     5461 tgctcgaaat gctcgatttg ccgttcgtga aggcgacggc gccggccctc tgcatgaggc
     5521 cgatctcggt catctcctcg ccacggagcc ccttggtcac ggccgccatg atgtgcacat
     5581 tgacgctggc attgtcgcgc cctcgtcgct ggatgtagtc gactagcgcg acctcgtcga
     5641 tgacaggcgc cgtatccggc atgcagatga tggtcgtcac gccgccggcg gccgccgcct
     5701 ggctcgcggt gcgcagggtc tcgcggtgct cctgacccgg ttccccggtg aacacctgag
     5761 cgtcgatcag tccggggcag agaacgtgac cgaggcagtc gacaatcgtc gcgccttccc
     5821 ctccgttgtg gcgaaacttt gtaccgagat cggcgatcgc gccgttgcgg acgaggacac
     5881 cgccgagttc gtcgcggccc gaggccggat caatcacgcg ggcgttgatg aagaccgttg
     5941 ctgccggcgt acccgggact ggcttgacca ttgggcgcct catcggttcg gcaggtgacg
     6001 cgcgagggcc tcgagcaccg ccatgcgcac ggcgacgccc atctccacct gatcgcgaat
     6061 gacgcttcgc gagtggtccg cgatcgtcga tgcaatctcg acaccccggt tcatcggtcc
     6121 tggatgcatg atcagcgcat cggccttggc gcgcccgagc ttctcgtgat cgaggccgaa
     6181 gaaatggaag tactcgcgtg tcgacgggac gaagcgtcca ccctgcatgc gctcgcgttg
     6241 caaccgcagc atcatgatga tatcgcagcc gtcgaggccc ttggacatgt cagtatagac
     6301 ttcgcagccg agccgatcgg cctgcggcgg cagcagcgtc gatggcgcga tcaggcggac
     6361 ccgcgcgccg agcgtgttta ggacgttgat gttcgagcgg gcaacccgcg agtgcagcac
     6421 gtcgccgcag atggcaaccg tcagcccctg aatgcggccc ttggcgcggc ggatcgtcag
     6481 cgcgtcgagc agagcttgtg tggggtgctg gtgtgcgccg tcgccggcat tgatgacgga
     6541 gcagtcgatc ttctgggaga gcagctcgac ggcgccggct gcatggtggc gcaccacgat
     6601 gatgtctggc cgcatcgcgt tgagtgtcat cgccgtatcg atcagcgtct cgcccttctg
     6661 catggacgaa gtcgcgacgt tcatgttcat gacgtcggcg ccgagccgct tgccggcgag
     6721 ctcgaacgag ctctgcgtgc gcgtcgaggc ctcgaagaac agattgatta gcgtacgtcc
     6781 tctgaggacg tcgcgctttt tgttgatctg tcggttggtc tcggcggcct tgtcggcgag
     6841 gtcgagaagc gatgtgatct ccgcggccga cagcccctcg catgtgagga ggtggcggcg
     6901 ggggaaaccg gagaggtgcg tgagggtgct cattaaaggc gcactcatag gccgatcgca
     6961 cgcacagcgc aacagccccg gcagatttgt catttaccga ggtgccgcgg ctcaagcgta
     7021 ccgcgtgctc aacgatgaag gcacgatcgc cactcatgaa cttggcctca cagcagtctc
     7081 agctgtccgt gccgctcgct cagcggcctc tcgaaaagat ccgtccgaag cttgagcgag
     7141 cgctcgtcga ggccaagcct gcgcgaagcg agcctgaaac gggtcccgat ctgttcagca
     7201 aagggtccag tgcctcgctg ccgcgttccg aaggccgaca cgtaatcacg tcccccgtgc
     7261 atggagcgga gcaccgagat gaccctggcc gcgcggtccg gaaaggcttc ggagagccac
     7321 tcgcggaaga gctccttgag ctcgaggggg aggcgcagga gcacgtagcc tgcatccgtc
     7381 gcccccgctc cccgtgcctc ggcgaggatc gcttcgatct cgctgtcgtt gatggccgga
     7441 acgatcggcg ccatcatcac ggtgacgggg atgccggccg cggcgagcga ccggatggta
     7501 tccacgcgtc gcccgggcgt tgccgcccgc ggctccatgc gccttgccac atcccgatcg
     7561 agggtcgtca gcgaaatcgc caccttggcg agccctttcg ccgccatcgg agccaggatg
     7621 tcaatgtcgc gcagcaccag tgccgacttg gtgacgatcc cgaccggatg attggtccgc
     7681 gacaggacct cgagcaccga gcgcgtgatg cgtcgctccc gctcgatcgg ctggtagacg
     7741 tcagtgaccg ctccgagcgc gatggtcttg cagaggtagc ccggcctcgc gagctcgcgc
     7801 tcgagtacct ccgccgcatt caccttggcg tagagcctgg tctcgaaatc gagccccggc
     7861 gagtggccga ggtaggcgtg cgtcggtcgt gcataacaat agctgcagcc atgttcgcag
     7921 ccccggtagg gattgatcga ctggtcgaag ctaatgtcgg ggctgtcgtt ggtcgaaatg
     7981 atggatttcg cctgctcaat cgcgacttcg gtcttgaagg cggccagtcc ctccagcgag
     8041 ccccagccgt cgtcgaagct ttcgcgcgcc gtccgctcga agcgacccga tgcgttgctc
     8101 aaagcgcctc gcccgcgcct tagatccaca gccacccgcg cagtcgctgt ttcctcattt
     8161 ggctccgtcg catccttcat gtcggccatc cggtcgtaac gagaacataa agagatcacg
     8221 acaataagaa caatgcaaga actttcatcg ccgccgcacg acaagcccgt cgccgcgtgc
     8281 aatcgcgtga caggcccgcc gtcatcgggc acagcaggag cggagcacca gcagccggcc
     8341 gggccatgac cgacatcgca acgcgcgtct acagccacac ctggaagatc gatccgatcg
     8401 tgcgctcggt gctcgacacc gatttctaca agctattgat gacgcagacg atcttccggc
     8461 ggcaccggca gcgccgcgtg accttcggac tgatcaaccg gacacagagc gtgccgctcg
     8521 cccgcctcat agacgagggg gcactgcgcg agcagctcga ccacatccga tcgctctccc
     8581 tgtcgcgcgg cgagagcacc tggctccgcg gcaacacatt ctacggcaag cggcagatgt
     8641 tctcgcccga gttcatcggc tggctcgagg agttccgatt cccgccttac aatctcgagc
     8701 ggcgcggcga tcaatacgag ctgaccttcg aggggtcttg ggtcgagacg acgatgtggg
     8761 agatcccggc gctcgccgtg atcatggagc tgcgttcgcg tgcggtgctg aaagacctcg
     8821 gcaagttcga actgcaggtc ctctatgcgc gcggaatggc ccgcgtctgg gagaaaatcg
     8881 agcgcttgaa acaaattgcc ggccttcgca tcgccgattt cggaacgcgt cggcggcacg
     8941 gctttctctg gcaggactgg tgtgtgcagg caatgcagga gggcctgccg tcgtcgttcc
     9001 tcggcacatc gaactgcctg atcgcgctcc gccgcgaagt cgaggcgatc ggcaccaatg
     9061 cgcatgagct gccgatggtc tacgcggcga tcgccgagac ggagcaacag ctctcggcat
     9121 caccttacca ggtgctcgcc gactggcagc aggactacga tggcaatctg cgcatcatcc
     9181 tgcccgatac gttcggctcg gacggctttc tcgcgcatgc gccggactgg gtgtcgctct
     9241 ggaccggcat gcgcatcgac agcggcgatc cgatcgaggg cggcgagagg gcgatcgcct
     9301 ggtggcgggc gcgcggccag gatccggcga cgaagctcct gatcttctcc gacggcctcg
     9361 acgttgccga gatcgaggcc atctcggcgc acttcaagga ccgcgtccgc ctcggcttcg
     9421 gctggggcac gctgctcacc aatgatttcc gcagcctcgt ggcggggcgc cagctcgatc
     9481 cgttctcgct cgtatgcaag gtcgtcagtg ccgacggacg ccctgcgatc aagctttccg
     9541 acaacccgac caaggccatg ggaccgacgg gcgagatcgc ccgctaccgg cgcgtgttct
     9601 cggccgagac cggccgcgcg atcccggtta ccgtttgatc tcgggctcga tgaggtcgtc
     9661 gccgagccca tgccggccct gcctatcctc gatctcgacg atccagatgt cgtcgtcgaa
     9721 gcgacgttcg gcggtcagca ggtcgtcgat ctccctctca ggctgatcga cgcggcgcgc
     9781 ctcgaagcgc cgttcggatg cagcctcgat catcccggcc atcgccggcc catagagaga
     9841 ggccgtgccg tcgagccggg cgaccttgat ccagatcgcc ccgccgcggt catcgccgcg
     9901 cttcacaatg aacgcgccca gaccggtcgc cgagcgctga cgcacatagg ccttgaccca
     9961 gagctcggag ggaatacgca cgctgctcag gctccgtttc ggcactccgt ttcggcaccc
    10021 ctgccgagat ttcaggctcg tcccgccgca tgaatcggcg cttgccacgt cggtcgcgtt
    10081 tgacaatggc ctcgcactga tcccattctc gatcgtcgat ttcaagcgtt gcgaatggac
    10141 ggggctaccc cgggcgcggc gcagtcgaag ggagaggacg atgggactga cgcgacgagg
    10201 cctactcgcc gtggcgggca gcattgccgc tgccgccatt tcggctcagg cacctccggt
    10261 gatggcgcag cagaaagtgc tgcgcgtggt gatgcactcc gacctcaaga tcgtcgatcc
    10321 gatctggacg acggcctaca tcacccgcaa ccacggctac atgatctatg acgtgctctt
    10381 cgcgcttgac gccaagggcg agatccagcc gcaaatggtc gacaagtacg aggtatcggc
    10441 cgacaagctc tcctacacct ttacgctgcg cgacgggctc acctggcacg acggcaagcc
    10501 ggtgacaggc gaggactgca tcgcctcgat caagcggtgg ggcgcaaagg acgccacggg
    10561 ccagaagctc atgaccttcg tcgctggctt cgaggctgtc gacgccaaga cgttccgcat
    10621 caagttgaag gagccgacgg gcctcgtgct ggtcggcctc tccaagccgt cgtcgtccgt
    10681 tcccttcatg atgccgaagc gcgtcgccga caccgacccc aataagcaga tcgaggactt
    10741 caccggctcc ggcccgttcg tcttcaagaa cgacgagtgg aagcccggcg acaaggtcgt
    10801 ctacgtcaaa ttcgacaagt acgtcccgcg caaggagccg gcctcgggcc tcgctggcgg
    10861 caagatcgtc tacgtcgacc gcgtcgagtg gcgcgccatc tcggaccacc agcaggcggt
    10921 caatgcgctg cttgccggtg agatcgacct catcgagagc ccgccgcacg agctctacga
    10981 actcatcaag aaggacagca aagtcgcgct gttcagctgg aacacgatcg gcaaccagta
    11041 caccttccgt ccgaactggc tcgccaagcc cttcgacaac ccgaagatca ggaaggcgct
    11101 ctggtacgcc ttcaaccagg aggatttcct gaaggcggtc atcggtgatc ccgaatacta
    11161 caaggtgtgc aaggccttct tcgtctgcgg cacgccgatg gcctccgaga agggcatgga
    11221 cggattgctc gaatcgaact tcgtgaaggc gcgcgagctc ctgaaggagg ccggctacga
    11281 cggcacgccg atcgtgctca tgcattcgac ggacctccag gtgctgacga acctggcgcc
    11341 ggtcgccaag cagctcatgg agaaggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccgagcgcg ggcggctggc atgccttcct
    11461 cacctcgtgg gtggcagcgg acatcatgaa cccggtgatg gccggctact tcaattcggc
    11521 ctgcgacaag gcgctgttcg gctggccctg cgaccccgag atggagaagc tccgcgacgc
    11581 gttcgcgcgc gagaccgatc cggcaaagct caaggcgatc gccgaagccg tgcaggtgcg
    11641 cgccactgag atgacgagcc acatccacct cggccagtgg tacagcgccg gcgcgcatcg
    11701 caagtcgatc agtggctggc tcgcgacgcc cgttccggtg ctctggaaca tcaagcgcgc
    11761 cgattgatgt cggatggggc gcgccgcggc ggctggcacg aagcgggccg acccgccttt
    11821 gagcacattc ggcattcgag ggacactgag cggcgagcga ccgaacggca tccgggtaag
    11881 cgtatggccc tctacatcct ccgacgcctg ctggcgacga tcccggtgat gctgatcgtc
    11941 gccattcttg tcttcctcat gctgcgcctc tcgcctgccg atcctgccgc gatcattgcg
    12001 ggcgatgcgg caactgccga ggacgttgag cgcatccgcg ccaagctcgg cctcgaccgg
    12061 aatatgttca gccagttctt catctggctc ggctcgatcc tcaccggcga tttcggcgag
    12121 agcttcttct acaagcagcg cgtcacgcag ctcatcggcg accgtatcgg cccgacgctc
    12181 gcgctctcga ccttcacgat cgtcatcgcg gtgctggtcg ccgtgccact cggcgtcgtc
    12241 gcggcctaca agcagggcag ctggatcgat cgcctcgtca tgggcttctc ggtgctcgga
    12301 ttctcggttc cggtcttcgt gacgggctac atcctgatct tcgtcttcgc catctggctg
    12361 aacctgctcc ccgtgcaagg ctaccagccg atctcggaag gcgtcggcgg cttcgtctac
    12421 aagctcgtcc tgccggcggt gactctgagc gtcgtctaca tcgcgctcat cgcccgcatg
    12481 acgcgcacca gcatgctcga ggtgctctcg gaggactacg ttcgcaccgc gcgcgccaag
    12541 ggccagatcg agcgcaaagt gctgttccgg catgcgctgc gcaacgccgc cgtgccgatc
    12601 gtcaccatca tcggcctcgg cgtcgcgctg ctcatcggcg gggcggtcgt gacggaatcg
    12661 atctacacca tcccgggcct cggcaacctc accgtcggcg ccgtgctcgc ccgcgactat
    12721 cccgtgatcc aggccgtgat cctgctcttt tcattcgcct acgtgatgat caacctcgcg
    12781 gtcgacatca tctacacgct gctcgatccg aggatccgct attgaccgtc acgagcccag
    12841 cgcccgaggc cgatgccgcc gccccgcggg ctacgacagc gagaaagctc gcgcgccacc
    12901 cgaacgtcgt ggtcggcagc atcatcgtca tgctgatcgc gcttctcggc ctgttcgcgc
    12961 cgctgctcgg caccaccgac ccggttctca acaatccctc gttcaagctg aagaagcccg
    13021 gcgccgaggc gaccatccgc aacgacgacg gcaccaagtc gacctacgtt cacaagctcg
    13081 gtaccgacag cctcggccgt gacgtgtgga gccgcatcgc ctacggtgcg cgcgtctcgc
    13141 tgatggtcgg cgtctcggtt gccgtactct cgattagcat cggcctcgtc atcggccttg
    13201 tcgccggcta catccgctgg ctggatttca tcatcatgcg catcatggat ggcttgatgg
    13261 cgatccctgc gatcctgctc gccatcgcgc tcgtctcgct gttccgcagg ggaagcggcc
    13321 tcctccccgt catcatcgcc atcgtcgtcc ccgacattcc gcgcgtcgtc cgcctcgtgc
    13381 gctccgttgt gctttcggtg cgcgaggagc cctatgtcga ggccgccgtc atctcgggga
    13441 cgccgacagc caagctgatc gtccgccaca tcctgccgaa caccgtttcg ccgctcatcg
    13501 tgcagggcac tttcatcgcg gccggcgcga tcctcgtcga agccgtcctc tccttcctcg
    13561 gcatcggcgt ctcgcccgac accccgacct ggggaaacat catggctgac gggcggacct
    13621 atttccgcat ccatccgcac cttatcctct atccgggaat cgtgctcgcg ctcaccgtgc
    13681 tcgccgtcaa catgctcggc gatggcctcc gcgatacgct cgatcccaag atggagcgac
    13741 gggtatgagc acgactgcgc ccggtcccaa tacggcctcg accactgccc cgattctcga
    13801 ggtcaccggc ctctcagtca gcctgccgaa aggcagcgac cggcgcttcg ccatcgagga
    13861 cgcctcgttc tcggtcgggc gcggagagat cgtgtgcgtc gtcggcgagt cgggctccgg
    13921 caagtccgtc accgcgcagg ccatcatggg cctcctgccc aagggccagc tcgcgatcac
    13981 ggccggctcc atccgcctcc tcggcgagga gaccgcgcag aactcccagg ccgagtaccg
    14041 ggcgctgcgc ggcacgcgca tggcgatggt attccaggag ccgatgacgg cgctcaatcc
    14101 ggtcgagcgc gtcggcgacc agattgccga ggtcttggaa attcacacac ccctctcggc
    14161 cggcgaacgc cgccagcgcg tcctcgatat catgcgcgcg gtgcacctcc ccgatcccga
    14221 gcagatgatc gacgcctacc cgcaccagct ctctggcggc cagcgccagc gcatcatgat
    14281 cgcggcagcc ctgatcctcg atccggccct gctcatcgcc gacgagccga cgaccgccct
    14341 cgatgtcacc acgcaggcgc agatcctgaa gctcgtccgc gagttgcagg agcggcgcgg
    14401 caccggcgtc ctcttcatca cgcacgactt cggcgtcgtc gccgagatcg ccgaccgcgt
    14461 cgtcgtcatg cagcacggcc gtatcgtcga gcagggcccg cgcgatgacg ttctgctccg
    14521 cccgcgcgaa gcctacacca ggatgctgat cgcctccgtg ccgagcctca cgccgccggc
    14581 gcgcgagccg aagaccggag acatctcgct ggtcacacgg gcgctcacca agacttacgg
    14641 cgccaaagga ttttttggcg gcgggcgggt tgtgcatgcc gctgccgacg tcaacatcga
    14701 ggtccggcgt ggcgagaccc tcggcatcgt cggcgaatcc ggttcgggga agtcgaccgt
    14761 cgcgcgctgc attgcccggc tgatcgatcc gacctctggc gagctccgcg tcgaaggccg
    14821 cgaaattgcg cgcgtgcctg aaagtgagct tcgcccttac cgccgcacca tccagatcgt
    14881 cttccaggac ccctatcgtt cgctgaaccc gcgccgcacg gtcggcgcct cgatcgtcga
    14941 aggcccgatc aacttcggcc agacaccaga tgccgcactc cagcgcgccc gcgagctcat
    15001 gacgctcgtc ggcctccgcc ccgacgcgct cgaccgctac ccccatcagt tctcgggcgg
    15061 ccagcgccag cgcatctgca tcgcccgcgc gctcgccatg gagccgaagc tgctgatcgc
    15121 ggacgaggcc gtttccgcgc tcgacgtctc cgtgcaagcg caggtcctcg ccctcctcga
    15181 cgacgtcagg aagcgcttcg acctcgccgt gctcttcatc acccacgacc tgcgcgtcgc
    15241 cgcccaggtg tgcgaccgca tcgccgtcat gaaatcgggc cgcgtcgtcg agcatggtgc
    15301 gaccaaggac atattcgccc gaccgcagca cgactacaca aaggccctgt tcgaggccgc
    15361 ccccggccgc ggcttcggca cgcgcgggct cgccgctgcc accgcagccc tccc
//
LOCUS       DAORNH010000075        15411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2925_length_15411_cov_2.974863, whole genome shotgun sequence.
ACCESSION   DAORNH010000075 DAORNH010000000
VERSION     DAORNH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2925_length_15411_cov_2.974863"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2443)
                     /locus_tag="PK264_09695"
     CDS             complement(<1..2443)
                     /locus_tag="PK264_09695"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HRD76198.1"
                     /translation="MIIFVDSGVENAEKIISAISGNAEIIFIDKSRDGMEQIAEAMTG
                     RTSVGAIHIVSHGDAGELRIGNSALTTSSIGAGHADELAVIRGALSADADILIYGCDV
                     AAGDKGQEFVAALARATGADIAASTDDTGAAALGGDWDLETRTGLIDTTGIYAPDYDG
                     LLAPLTITPVGTGGVTATSLAQQLMGSDVTIVSATLSGQTYQAGVFSGATGYAPSWLA
                     FDSGVVFSSGSASGLPTSNTADDYSAGIITNSGGFAPFGTIGGNTSYNASYIDISFIP
                     DNNRISIQFVFGSDEYNEYVYGAFSDAIAIWVNGTNIAVTPTGAPISIDTINQAGTYT
                     PTFGTPGASAAPSLFVNNDLQNGGGTIPIEMDGFTRTLGATITVNPGIVNTIRLGVSD
                     IGDGFLDSWLLVKADSVQANLIAQNDTVGTTLNTPVTFDVLANDIDFDGDAKTIVNIA
                     DQSITTGQTLTLASGATVKLNADQTLTVTPALNSTNQELFTYTITDSLGNTATAYATV
                     NIVAGTPPTLDLDASAAGTGFATTYTVGGGGISIADTDATVTDSNNIARATISLGGSF
                     AGDLLTAGTLPAGVTVDPSSTATSIVLVGNVTPATMATAIKAVSFSNSSGSPDLTDRL
                     ISVQVTDATGLSSAIAQTTISLVVPNAPPVAADDTLSTNEDTLLATNVITANGIDSDP
                     DNDPLTIVSATIDIDGDGNPDALPLGTATPIIVGGNTIGTLSLSVGGGLIFMPATNWT
                     GTVPSLTYTLSDGNSLTDTATVSITVTPVNDPPIATPSTSSGNEDTAVAVDLVGTDVD
                     GTIAAVTVTTLPPLGE"
     gene            complement(2953..5067)
                     /locus_tag="PK264_09700"
     CDS             complement(2953..5067)
                     /locus_tag="PK264_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRD76199.1"
                     /translation="MQDITLPELRAELALLRGAPTATGAPTWLIHDPVQGRFLQIDGA
                     AYHALAHWRTCRSADELASRAAADDSYAITAEQIGQLIGFLEAQKLTTEPARGGWRHF
                     AAERARSRHSVLALLVHNYLFFRVPLWRPAGFLAKTMPLALLVASRPAWVLYWVLSIA
                     GLYLASREWDLVAQTLLPPTTWEGIALIAVSIGVVKLAHELGHAYVAAGFGCRVPTMG
                     IAFMLLAPLPYTDVTDAWRLRDRSKRIAIDSAGIKVEAVIAGLALFLWAFLPLGAAKT
                     AMATIAAVGLVSTLLVNLNPFMRFDGYYLLAEHLAIENLQPRAFDLGVWKLREVILGL
                     GQPPPEVLPRRRVATLVAYAWATWVYRLLLFVAIALVVYHYFFKLLGIVLFAIEIIYF
                     VARPVMGELKVWWRMRSAIVRSRRAAISAVVMCGGLFIAALPWSTRIEIPAVLEADRT
                     QQVHAPRVALVKMLHVVAGQSVSEGMPLATLAAPDIDREIDIQRSRLRLAELQYARRG
                     ASSADREASLVLETTIASLKSRIAGLEEEQDLLILRAPFAGEIREFEPELHAGRWVAP
                     RQPLMTITRGHGLVIRGYVAEADVRRIAPGDRGKFIPDHAQRPVLRLRVEDIAVGAAA
                     AIEIADLSATHSGRIGVSLDERRRDVPTIAHYLVRMTVLDDLVPADLSVRGIVIVEGA
                     AESPLARLWRSALKVIIRESGL"
     gene            complement(5069..6379)
                     /locus_tag="PK264_09705"
     CDS             complement(5069..6379)
                     /locus_tag="PK264_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRD76200.1"
                     /translation="MALLQLEAELRKVRSKAELAYFVANEFRRPTRSQQIIAIERVSG
                     GKLRVAAITALTHVDRASPMIRWIEQILASLGQSRRLIEPQEFEASAFAEISPEAGAS
                     YPLNQMMWLPFLNFDGAVSGGMIMARIAPWTEHEATIGRHLADAAAFATAALGRREGR
                     GARAFTRGAGALLAALVVVAAGIIPVPMSALAPVEVASREPTVVTAGLEGVIETIAID
                     PNMPVVKGTVIARFADTVLRNRLEVAEREVVVAQSRLRKAAQLAFVDLRGRQELALAE
                     AELEVKSAERDFAREMLDRATIVAPRSGTALFTDRKELIGRPLVAGERIIEIADPNDV
                     QFRVDLPVAEAIVLKDGARVKVFLDSDPLRPVEARLMRADYQAKVRDGQPLAFRVVAV
                     VDGPRPEHVRLGVRGTAQVYSDEVPLAFYLLRRPFTAVRQWLGI"
     gene            complement(6514..7230)
                     /locus_tag="PK264_09710"
     CDS             complement(6514..7230)
                     /locus_tag="PK264_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD76201.1"
                     /translation="MLPTAGAQSLTRSGTPLPIRGIVRPLQQAMIAADISARIAAIRF
                     REAQGFKKGDRLVEFDCERLEAEAAAADALWRETKLSLESNSYLEKRGAAGRIDVEIS
                     RARADKAGSEAAALKARLKQCVIIAPYDGRVAELTINEHEFPSAGKPFISIVATGEFE
                     IDLIVPSHWLKTLTPGATFTFAVDETGQSHAAHVLRIGAAVDPVSQTVKVIATFLEKG
                     RAVLPGMSGTATFAKTDGAP"
     gene            complement(7601..8812)
                     /gene="coaBC"
                     /locus_tag="PK264_09715"
     CDS             complement(7601..8812)
                     /gene="coaBC"
                     /locus_tag="PK264_09715"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRD76202.1"
                     /translation="MKRILLVIGGGIAAYKCLDLIRRARERGLAVRTVMTRAATEFVT
                     PLSVAALSNERVLTELFDLDEEREIGHIRLARDCDVIVVAPATADLLARMATGQADDL
                     ATAVLLATDKPVLAAPAMNPRMWSHAATQRNIATLGRDGVRFIGPRSGEMAERGEAGL
                     GRMAEPAEILEAIEDALSPGASRGPLAGRHVLVTAGPTHEPIDPVRYIANRSSGKQGY
                     AIAMACRDLGAAVTLVSGPTALPQMAGVSHVDVESARDMLAAVQAALPADIAIFAAAV
                     ADWRIANAAGEKIKKGKSGAPKLELAENPDILRSVASLPAGSRPALVVGFAAETEKVI
                     EHAGAKLRRKGCDWIIANDVSPATGIMGGDRNAVHLLSAAGVESWPEMDKDAVARRIA
                     ERAARYVAALA"
     gene            complement(8870..>9068)
                     /locus_tag="PK264_09720"
     CDS             complement(8870..>9068)
                     /locus_tag="PK264_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRD76203.1"
                     /translation="VTELEIAAANVRQLFAELARRYPAIAPHLEEGLAVAIDGQIYQD
                     ALLEPINAGAEVHILPQIAGG"
     assembly_gap    9069..9088
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9117..10385)
                     /gene="ispG"
                     /locus_tag="PK264_09725"
                     /gene_synonym="gcpE"
     CDS             complement(9117..10385)
                     /gene="ispG"
                     /locus_tag="PK264_09725"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRD76204.1"
                     /translation="MSDTGRPRRSSVSVDVGGVMVGGAHPIVVQSMTNTDTADSGATA
                     TQVAALARAGSEIVRVTVDRDEAAKAVPLIRDRLLKAGVKVPLVGDFHYNGHKLLADN
                     PACAEALDKYRINPGNVGFKDKKDRQFSSIVELAIKHEKPVRIGVNWGSLDQELLTHL
                     MDENARAAAPNPARSVMHEAIVQSALLSAARAEEIGLGAERIIISAKVSAVQDLIAVY
                     TMLADRCRYALHLGLTEAGMGSKGIVASSAAMGILLQAGIGDTIRFSLTPEPGGDRTL
                     EVRAAQELLQTMGIRAFMPIVAACPGCGRTTSTVFQELARDIQAMIADRMPEWRRDYP
                     GVETLQLAVMGCIVNGPGESKHADIGISLPGTGEQPAAPVFIDGKKAMTLRGPNIAAE
                     FKTVVESYIARRYGGGAAAETRAGGSVRHG"
     gene            complement(10410..11207)
                     /locus_tag="PK264_09730"
     CDS             complement(10410..11207)
                     /locus_tag="PK264_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRD76205.1"
                     /translation="MLEPFIIRAILAGVGLAIVAAPLGCLVVWRRMAYFGSTVAHAGL
                     LGVALGLVAGIDLTVGIIIVSLLIGVLLIALERQKVLPIDTLLGLLSHAALAGGLVAA
                     SKLAGQRLDLIGYLFGDILAVSRADLLWIWGGGGLVLAVTGYLWPTLVATTVHAEIAA
                     AEGHGTGRAEAAMVMLIAFTIAIAMKIVGVLLVIAFLVMPAAAARPFAATPERMAVAA
                     AVIGALGTIAGLLLSVGFDIPTGPAIVLVLSAAAFAALLLSIVHSED"
     gene            11366..11812
                     /locus_tag="PK264_09735"
     CDS             11366..11812
                     /locus_tag="PK264_09735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD76206.1"
                     /translation="MSSAVAEIDRRIRESFAKQGLMTTLGAEVTHVGVGEAVIELRPR
                     PAISQQHGFVHAGAVSAIADSAAGYAALSLMPANRGVLTTEFKINLLAPAAGERIIAR
                     GRVVKAGRALTLAQTDVVALADGREKLIALLTATFMSIEGREGIAD"
     gene            11833..12249
                     /locus_tag="PK264_09740"
     CDS             11833..12249
                     /locus_tag="PK264_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD76207.1"
                     /translation="MQIEVETTVDGTIAEVWRAYTSPGDIMQWNAASDDWHTPSASVD
                     LREGGKFGSRMEARDGSQGFDFEGTYTKIIEHQLIEYAFGDRTAKVEFIPEAGGVRVR
                     VAFDPDQTYPPDIQRAGWQAILDNFARHVGAKRRRQ"
     gene            12347..13216
                     /locus_tag="PK264_09745"
     CDS             12347..13216
                     /locus_tag="PK264_09745"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HRD76208.1"
                     /translation="MTAPRIDDYFVCVGAQKSGTTWLARILADHPELFLTPVKEIHYF
                     DHVRGITGHLSMEKRRSRYRKYHQRLWTEWGRFSRNRQHWRWYRDYMRPEIGDRWYAD
                     LFRHRDGRRFAGEVTPEYAIIGREGFEHIRRLAPAARVLFIMRNPVERAWSQALHHAR
                     SERIDAGRASLEELKRIVDQPRFRALGDYGATLEAMSAVFPAEQSLAMFYEDIHKDRP
                     AALEAVCRFIGIRFDPALLGDTGRRFNKSQDAKLPAELRVHLAKCYKATAEDVRRRLG
                     RIPDAWAKEFGLP"
     gene            complement(13241..13501)
                     /locus_tag="PK264_09750"
     CDS             complement(13241..13501)
                     /locus_tag="PK264_09750"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HRD76209.1"
                     /translation="MLPLLTVSGPATLEAGALWRLPEGQSLKLLALDGTLWMSQPQEP
                     GDIIVEAGQSIELDVAGLTIVEAIGQQAKVWISVGEAARIAA"
     gene            complement(13513..13713)
                     /locus_tag="PK264_09755"
     CDS             complement(13513..13713)
                     /locus_tag="PK264_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76210.1"
                     /translation="MNTNEFPYKYPLSAEDLAAIRLKAHAERNAAVVSLTRSIGAALA
                     WPFRLLKSPTKTGHADCEAPAR"
     gene            13812..14780
                     /gene="gcvA"
                     /locus_tag="PK264_09760"
     CDS             13812..14780
                     /gene="gcvA"
                     /locus_tag="PK264_09760"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HRD76211.1"
                     /translation="MRHALPPLNALRAFEAAARHLSLTKAAEELHVTPAALSHQVRGL
                     EEALGVRLFQRLPRALALTDAGRKLLPGLSSGFSQIRQAVALVQETGGGRVLVVSTPP
                     GFTSKWLAPRLHRFLTANPDIEVRVSSTLKNADFETDGVDVAVRNLRHSQPVDQSLFS
                     EHLVDLGLVPVCSPAFLAAHPELVSPSDLAKVPLVHDNQMAGRLDNPGWPEWLAAAGV
                     DGVSLGHGVKFNSADHALEAALEGAGVLLAHSVLASDDLKVGRLVSPFPMTIAMRRSF
                     RIVCPIGHETRANIRTFRDWIHAEMAASGGRRFDVQRRAGLPAAGM"
     gene            complement(14744..15019)
                     /locus_tag="PK264_09765"
     CDS             complement(14744..15019)
                     /locus_tag="PK264_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HRD76212.1"
                     /translation="MSIALGIALYFVIWWTVLFAVLPFGVRTQEEAGSVVPGTPESAP
                     MAPKLKKVFLITTLVAAVVFAIVWTEVTYRVVGVLDYIPAAGSPARR"
     gene            complement(15038..>15411)
                     /locus_tag="PK264_09770"
     CDS             complement(15038..>15411)
                     /locus_tag="PK264_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76213.1"
                     /translation="EGLMRRAVVTRRSNDTGGAVLRSGERELATLGSLRADDDGTMAS
                     TFTHRPAFADVADEMRRLAAASGDEALAIAAEIDRAGVHVWHSVHNMRIDRPGSIRLV
                     KGEIRFAPTDAFLVMRSGGLG"
BASE COUNT         2681 a         5085 c         5019 g         2606 t
ORIGIN      
        1 cctcgccgag cggcggcaag gtcgtgacgg tgacggcggc gatggtgccg tccacgtcag
       61 tgccgacgag gtcgaccgct acggccgtat cctcgttgcc cgagctggtc gacggcgtgg
      121 cgatcggcgg atcgttgacc ggcgtgactg taatgctgac cgtcgccgtg tcagtcaggc
      181 tgttgccatc gctgagcgtg taggtcagcg atggcactgt gccggtccaa ttcgtcgccg
      241 gcatgaagat caggcctccg cccacgctga gcgacagcgt gccgatcgtg ttgccgccga
      301 cgatgattgg cgtcgcggtg ccgagcggca gtgcgtcggg attgccatcg ccgtcgatgt
      361 cgatggtggc ggagacgatg gtcagcggat cgttatcggg atcgctgtcg atgccgttcg
      421 ccgtgatgac gttggtggcg agcagcgtgt cctcattcgt cgacagcgtg tcatcggccg
      481 cgaccggtgg cgcgttggga acgacgagcg agatcgtggt ctgcgcgatc gcggacgaga
      541 gaccggtcgc gtccgtcacc tgcacggaga tcaggcgatc ggttagatcg ggcgagccgc
      601 tggagttcga gaagctcacg gccttgatgg cggtcgccat cgtcgccggc gtgacgttac
      661 cgacgagcac gatgctggtc gccgtggagg acggatcgac tgtgacgccg gcgggcagcg
      721 ttccggcggt cagcaggtca ccggcgaacg agccgccgag gctgatcgtg gcgcgcgcga
      781 tgttgttgga gtccgtgacc gtcgcgtcgg tgtcggcgat cgaaatgccg ccgccaccaa
      841 cagtgtaggt cgtcgcgaac ccggttccgg cagcggacgc atcgaggtcg agcgtcggcg
      901 gcgtgccggc gacgatgttg acggtggcgt aggccgtcgc cgtgttcccg aggctgtcgg
      961 tgatcgtgta ggtgaagagc tcctggttgg tcgaattgag cgcaggtgtg accgtcagcg
     1021 tctggtcggc gttgagcttc accgtcgcgc cgctcgccag cgtcagcgtc tggccggtcg
     1081 tgatggactg gtcggcgatg ttgacgatcg tcttggcgtc gccgtcgaag tcgatgtcgt
     1141 tggcgagcac gtcgaacgtg acgggagtat tgagcgtcgt gccgacggtg tcgttctgcg
     1201 cgatcaggtt ggcctggacg ctgtcggctt tgacgagcaa ccatgagtcc aggaagccgt
     1261 cgccgatgtc ggacacgccg agccgtatgg tgttcacgat gccgggattg accgtgatgg
     1321 tcgcgccgag cgttcgcgtg aacccgtcca tctcgattgg gatggtgccg ccaccgttct
     1381 gcaggtcgtt gttgacgaag agtgaaggcg ccgcggaggc gcccggtgtc ccgaaggtcg
     1441 gcgtgtacgt gccggcctgg ttgatggtgt cgatgctgat gggtgcgccg gtcggcgtca
     1501 cggcaatgtt ggtgccgttg acccagatcg cgatcgcatc ggagaacgcg ccgtagacgt
     1561 actcgttgta ctcgtccgag ccgaacacga actggatcga aatgcgattg ttgtccggga
     1621 tgaacgagat gtcgatgtac gaggcgttgt aggaggtatt gccgccgatc gtgccgaatg
     1681 gtgcaaagcc tccgctattg gtaatgatcc cggccgaata gtcgtcagct gtgttggacg
     1741 tcggcaggcc cgaagcactg ccggacgaga atacgacgcc ggaatcgaag gcgagccagc
     1801 tcggcgcata gcccgttgcg ccagaaaaga cgcctgcctg gtacgtctgg ccggagaggg
     1861 ttgcgctgac gatcgtgacg tccgatccca tgagctgctg ggcgaggctc gtcgccgtga
     1921 cgccgcctgt gccgaccggc gtgatcgtca gcggggcgag caggccgtcg tagtccggcg
     1981 catagatgcc ggtggtatcg atcagccccg tgcgcgtctc gaggtcccaa tcgccaccga
     2041 gcgccgcggc gccggtatca tctgttgacg cagcaatgtc cgcgccggtt gcgcgcgcca
     2101 gggccgcgac gaattcctgc cccttgtcgc ccgccgccac gtcgcagccg tagatcagaa
     2161 tatcggcgtc ggcgctcagc gcgccgcgaa tgacggcaag ctcgtcggca tggcccgcac
     2221 cgattgagct ggtcgtcagc gcactgttgc cgatgcgcag ctcgccagca tcaccatgcg
     2281 agacgatgtg tatggcgccg acgcttgtgc ggccggtcat cgcctcggcg atctgctcca
     2341 tgccatcgcg cgacttatcg atgaagatga tctcggcatt gcctgaaatt gcagaaatga
     2401 tcttctcagc attttcaacg ccgctatcca cgaaaatgat cacgcccgaa gcttcgccgt
     2461 tcgaaggcgc gacaggggcc gcagcgaggg ccgcagccag atcggttgtg tccaccgctg
     2521 cacctggggc cattgtcgtc tgttcagaag gtaccgacgc ctcggctgca tcgcggtgct
     2581 ctgcgatggt gtcggccgcc gcgccgtcga acacaatccg ctgctcgagc gcccgaaagg
     2641 acgaggaact cagcaagtgc tgagcttgcg tcaccgctgc gtgcgatttc gcatggccgg
     2701 tgcgattggc ggcattcggg cggcgatcac gtcgcttcat ggctggatat ccgagaggtt
     2761 atatatttgc cccaagtcgg cagaaaccga catctgcgga tatctcacat gaattcaatg
     2821 aagtatgcgt aaatcgcaat aatcgactta ccgtcttcgc aactgcgcgc tatttcagcg
     2881 gattttatga aagaaggatg tcgaaatcgg taatagtccc atcgccaact attgctaaga
     2941 ccttgcccgg cgtcataggc ccgattctcg gatgatgact ttgagggcgc tccgccagag
     3001 ccgggcaagc ggactttccg ccgccccctc gacaatcacg atgccgcgca ccgaaagatc
     3061 cgccggcacc aagtcgtcga gcactgtcat gcgaacgaga tagtgcgcga tcgtcggcac
     3121 gtctcgccgc cgctcgtcga gcgaaacgcc gatccggccc gagtgcgtgg ccgacaggtc
     3181 agcgatctcg atggctgccg cggcgccaac ggcaatatcc tcgacgcgca atcgcagcac
     3241 cggccgttgc gcgtgatcgg gaatgaactt tcccctgtcg cctggcgcga tccggcgcac
     3301 gtccgcctcg gcgacataac cccggatgac gagcccatgg ccgcgcgtga tggtcatcaa
     3361 cggctgacgc ggcgcgaccc aacgccccgc atgcaattcc ggttcgaatt cacggatctc
     3421 gcccgcgaat ggagcgcgca ggatgagcag atcctgctcc tcctcgaggc cggcaatcct
     3481 ggacttgagc gatgcaatgg tcgtctcgag aacgaggctc gcctcacgat ccgcgctgct
     3541 tgccccgcgc ctcgcatact gcagctcggc gagccgcaac cgtgatcgct gaatgtcgat
     3601 ctcgcggtcg atgtctggtg ccgcgagcgt cgcaagcggc atgccttcac tgaccgactg
     3661 tcccgccacg acatgcaaca tcttgaccag cgcgactcgc ggcgcatgga cctgctgcgt
     3721 ccggtccgcc tccaacactg ccgggatttc gatccgcgtc gaccagggca gcgcggcaat
     3781 gaagagaccc ccgcacatga caacagcgga gattgcggcg cgccgggagc gcacgatcgc
     3841 ggaccgcatc cgccaccaca ccttgagctc tcccatgaca ggcctcgcga cgaagtagat
     3901 gatctcgatg gcaaacagca cgatgccgag cagcttgaag aagtagtggt agacgacgag
     3961 cgcgatcgcc acgaatagga gaagcctgta gacccacgtg gcccaggcgt aggcgacgag
     4021 cgttgcaacc ctccgccgcg gcagaacttc aggcggcggt tggccgagcc cgaggatgac
     4081 ctccctcagc ttccacactc cgaggtcgaa tgcgcgcggc tgcaggttct cgatcgcgag
     4141 atgctcggcg agcaggtagt agccgtcgaa acgcatgaac gggttcagat tgacgagcag
     4201 cgtactgacg agcccgaccg cggcgatcgt cgccatcgcc gtcttggcag cgccgagcgg
     4261 caggaacgcc catagaaaca aggcgaggcc cgcgatcacc gcctcgacct tgatgccggc
     4321 gctgtcgatg gcaatccgct tgcttcgatc gcggagccgc caggcatccg tgacatcggt
     4381 gtaaggcagc ggtgccagca gcatgaacgc aatgcccatc gtcggcacgc ggcagccgaa
     4441 gcccgccgcc acgtaggcat ggccgagctc gtgggcgagc ttcaccacgc cgatcgagac
     4501 cgctatcagc gcaatgccct cccacgtagt gggcggcagc agggtctgtg cgacgaggtc
     4561 ccattcgcgc gaggcgagat agagccctgc gatcgaaagc acccagtaga gaacccaggc
     4621 cggacgcgag gcgacgagga gcgcaagagg catcgtcttg gcaagaaagc cggcgggtcg
     4681 ccagagcggc acccgaaaga agaggtaatt gtgcacgagc aaggcgagca ccgagtgccg
     4741 gctgcgggcc cgctcggccg cgaagtggcg ccagccgccg cgagcgggtt ccgtcgtcag
     4801 cttctgcgcc tcgagaaagc cgatcagctg gccgatctgc tcggccgtga tggcgtagct
     4861 gtcgtcggct gcggctcggc tcgccaactc gtcagcgctg cggcacgtcc gccagtgcgc
     4921 gagcgcgtgg taggccgccc cgtcgatctg caggaaccgg ccttggacgg gatcgtggat
     4981 gagccatgtc ggcgcccccg tcgcggtcgg cgctccccgg agcaaggcca actcggcacg
     5041 cagctctggc agcgtgatgt cctgcatgtc agatgccaag ccactgccgc accgccgtga
     5101 acggccgcct tagcaggtag aaggcgagcg gcacctcgtc tgagtagacc tgggcagtcc
     5161 ccctcacgcc gaggcgaacg tgctcggggc gaggcccatc caccaccgcg acgacccgga
     5221 atgcgagcgg ctggccatcg cgcaccttcg cctggtagtc ggcgcgcatg agccgcgcct
     5281 cgacgggcct cagcggatcg gaatcgagga acaccttcac ccgcgcaccg tctttcagca
     5341 cgatcgcctc cgccacgggc aggtcgacgc ggaactgaac gtcgttgggg tcggcgatct
     5401 cgatgatgcg ctcgccggcg acgagcggcc gcccgatcag ctccttgcga tctgtgaaca
     5461 gcgcggtgcc ggagcgcggc gcgacgatcg tcgctcggtc gagcatttcg cgcgcgaaat
     5521 cccgctcggc cgacttcacc tcgagttcgg cctcggcaag tgccagctcc tggcgccccc
     5581 gcagatcgac gaaggcgagc tgggccgcct tgcgcagcct agactgggcg acgaccacct
     5641 cgcgctcggc gacttccaac cggttgcgca gcaccgtatc cgcaaacctg gcgatcacgg
     5701 tgcccttcac gaccggcatg ttgggatcaa tcgcgatcgt ctcgatcacg ccttcgagac
     5761 cggcagtcac cacggtcggc tcgcgggatg cgacctcgac gggcgccagc gccgacattg
     5821 gcaccggaat gatacctgct gcgaccacga caagtgctgc cagcagcgcg ccggcaccgc
     5881 gtgtgaaggc gcgcgctccg cgaccctcgc ggcgaccgag cgcggcagtg gcaaaggcgg
     5941 ccgcgtcagc gaggtgccgg ccgatcgtcg cctcatgctc cgtccaggga gcgatccgcg
     6001 ccatgatcat gccgccgctg accgccccgt cgaaattcag gaacggcagc cacatcatct
     6061 gattcaacgg ataactcgcg ccggcctcgg gcgagatttc ggcgaaggcg gaggcttcga
     6121 actcctgcgg ctcgatcagt ctccggctct ggccaaggct cgcgaggatc tgctcgatcc
     6181 agcggatcat aggcgacgcc cggtcgacgt gggtcagcgc cgtaatggcc gcgacgcgca
     6241 gcttgccgcc tgacacgcgt tcgattgcga tgatctgctg cgagcgcgtc ggccgtcgga
     6301 actcgttggc gacaaaatag gcgagctccg ctttcgaccg caccttgcga agctccgcct
     6361 cgagctggag gagggccatg gcagccgcgc ttgcggcgcc cccctgcgat gaaggcggca
     6421 aggcagactg gctcgccgcc gccgctgctc ctgcacctgt acctgtacct gtacctgcaa
     6481 aggctgaagg atgcggcact ttctcggcag tgatcatggt gcaccgtcgg tcttggcgaa
     6541 ggtcgccgtg ccgctcattc ccggcagcac agccctgcct ttctcgagga atgtcgcgat
     6601 caccttgacg gtctggctga cgggatcgac ggcggcgccg atgcgcagga cgtgcgcggc
     6661 atgcgattgc ccggtttcgt cgacggcgaa cgtgaaggtc gctcccggag taagcgtctt
     6721 cagccagtga gacggcacga tcagatcgat ctcgaactcc ccggtcgcca cgatcgagat
     6781 gaagggcttg ccggccgacg ggaactcgtg ctcgttgatc gtgagttccg cgacccgacc
     6841 gtcgtagggt gcgatgatca cgcattgctt gaggcgggcc ttcagggccg cggcctcgga
     6901 gccggccttg tcggcccggg cgcgcgagat ctcgacgtcg atccggcccg ccgcgccgcg
     6961 cttctcgaga tatgaattgc tctcgagcga cagcttcgtc tcgcgccaaa gcgcgtccgc
     7021 ggcagcggcc tcggcctcga ggcgctcgca gtcgaactcg acgagcctgt cacctttctt
     7081 gaagccctgc gcctcgcgaa agcggatcgc tgcgatccgc gccgagatgt cggctgcgat
     7141 catcgcttgc tggagcgggc ggacaatgcc gcggatcggc agcggcgtac ccgatcgggt
     7201 cagcgactgc gcgccggccg ttggcagcat cgtgctgctg cttcccacta tgagcagggc
     7261 tgctacggca gcgatcactt gcttccagcg catcttgctt gccccaaagc tttgaccgac
     7321 agcaggcgcg atccatcgac ccgatggacg caatcaaacg aattcgtcga ctcgcgtcaa
     7381 agcctgtcgt cccaggacgc ttgaccggcc atgccgcacg cctcggtcat caccgtcggt
     7441 cagcgcgttg ttatatcgat acgtttagag acatcgcgaa tcgagacttg acgcgaggag
     7501 gcgcgagact ggtcatagat tgagtatgct gaaaatatcg tttattgggt cagatgctac
     7561 attttcgcac ttgtggccgt tttcacacgg catcctctcg tcacgcgaga gcggcgacat
     7621 atcgggcggc tcgttcggca atccggcgcg cgaccgcgtc cttatccatc tctggccagc
     7681 tctcgacgcc cgccgcgctg aggagatgga cggcgttgcg atcaccaccc atgatgcctg
     7741 tcgcggggct cacgtcgttg gcgatgatcc agtcgcaccc cttgcgccta agcttggcgc
     7801 cggcgtgctc gatgaccttc tcagtctctg ccgcgaagcc gacgacgagc gccggtctcg
     7861 acccggccgg cagcgacgcc accgaccgca ggatgtccgg gttctcagcg agttcgagct
     7921 tcggcgcgcc ggacttgccc ttcttgatct tctcgccagc ggcgttcgcg atccgccagt
     7981 cggccaccgc cgccgcaaag atcgcgatgt cggcagggag cgccgcctga accgccgcga
     8041 gcatgtcgcg cgcactttcg acatcgacgt gagaaacgcc ggccatctgc ggcaatgccg
     8101 tcggccccga gacgagcgtc accgcagcac cgagatcgcg gcaggccata gcgatcgcgt
     8161 agccctgctt gcctgacgag cgattggcta tgtagcgcac ggggtcgatc ggctcgtgcg
     8221 tcggcccggc cgtcacgaga acgtgccgac cagccagcgg accacgcgaa gcccctggcg
     8281 acaatgcgtc ctcgattgcc tcgagtatct cggccggctc cgccatgcgc ccgaggccgg
     8341 cttcgccccg ctcagccatc tcgcccgagc gcggtccgat gaatcggacg ccgtctcgac
     8401 cgagcgtcgc aatgttgcgc tgtgtggcgg cgtgcgacca catgcgcggg ttcatggcgg
     8461 gtgcggcaag taccggcttg tcggtcgcga gcagaaccgc agtcgcgaga tcgtcggcct
     8521 gccccgtcgc catgcgcgcg aggaggtccg cggttgcggg agcgacgacg atcacgtcgc
     8581 aatcgcgtgc gaggcggatg tgtccgatct cgcgctcctc gtcgaggtcg aacagctcgg
     8641 tcaggacgcg ctcgttcgag agtgccgcaa ctgagagcgg cgtcacgaac tctgtcgccg
     8701 cccgtgtcat cacggtgcgc acggctaggc cccgctcgcg cgcccgccgg atgagatcga
     8761 ggcatttgta ggcggcgatg ccgccaccga tgacgaggag gatgcgcttc atgggaggcc
     8821 ttcaggcgcc aagtgtgagg gacggccaat cgatacgcgc cgggcgggat cagccgccgg
     8881 cgatctgcgg gagaatatgc acttccgcgc ccgcgttgat cggctcgagt agcgcatcct
     8941 ggtaaatctg accgtcaatg gcgaccgcga gtccctcctc gaggtgcggg gctatcgcgg
     9001 gatagcggcg ggcaagctcg gcgaacagct ggcgcacgtt ggcggcagcg atttcgagct
     9061 cggtgacgnn nnnnnnnnnn nnnnnnnnct ggcgcaggtt gccgatgagc acgaccttag
     9121 ccatgacgca cgctcccgcc cgctctggtt tccgcagccg cccccccacc atatcgccgc
     9181 gcgatgtagc tctcgaccac ggtcttgaac tctgccgcga tattggggcc acgcagcgtc
     9241 atcgccttct tcccgtcgat gaagacgggt gctgccggct gctcgccggt gcccgggagc
     9301 gagatgccga tgtcggcgtg cttcgattcg cctggtccgt tgacgatgca gcccatcacc
     9361 gcaagctgca gcgtctcgac gcccggatag tcccgccgcc actccggcat gcggtcagcg
     9421 atcatggcct ggatgtctcg cgcaagctcc tggaacacgg tcgaggtcgt ccgcccgcat
     9481 cccggacagg ccgcaacgat cggcatgaag gccctgattc ccatcgtttg caggagctcc
     9541 tgtgcggcgc gcacctcgag ggtccgatcg ccgccaggct caggcgtcag agagaagcgg
     9601 atcgtatcgc cgatgccggc ctgcaggaga atgcccatgg ccgccgacga agcgacgatg
     9661 cctttcgacc ccatgccggc ctcggtgagg ccgagatgca gcgcatagcg gcagcgatcg
     9721 gcaagcatcg tatagaccgc gatcagatcc tgcaccgccg acaccttcgc ggagatgatg
     9781 atacgctcgg ctccgagccc gatctcctct gcccgcgctg ccgagagcag cgccgactgc
     9841 acgatcgcct cgtgcatgac gctgcgtgcg ggattcggcg cggcagcgcg cgcgttctcg
     9901 tccatcaggt gcgtgagcag ttcctggtcg agcgagcccc agttgacgcc aatccggacg
     9961 ggcttctcat gcttgatcgc caactcgacg atcgaggaaa actgccggtc cttcttgtcc
    10021 ttgaagccaa cgttgccggg gtttatgcga tacttgtcga gcgcctcggc gcaggccgga
    10081 ttgtcagcca gcagcttgtg gccattgtaa tggaaatcgc cgaccagcgg caccttgacc
    10141 cccgccttca gcaggcgatc gcggatcagc ggcacggcct tcgccgcctc gtcacgatcg
    10201 acggtgacgc gcacgatctc ggagccggca cgggcaaggg ccgcgacctg cgtcgccgtc
    10261 gcgccactgt cagcggtatc cgtgttggtc atcgactgaa cgacgatcgg atgcgccccg
    10321 ccaaccatga cgccgccgac gtcgacggaa acagagctgc gccgcggcct gccggtatcc
    10381 gacatattgg gtctccgtcc gcctctcgtt caatcctcgg aatgcacgat cgaaagcagc
    10441 agtgctgcaa acgcggcagc cgaaagtacc agcacgatcg ccggccctgt cggaatatcg
    10501 aagccgacag acagcagcaa ccccgcaatg gtcccgagcg ccccgatgac cgccgccgcc
    10561 acagccatgc gctctggagt cgccgcaaag ggccgcgcgg cggccgccgg catgaccagg
    10621 aaggcgatca ccaggagcac gccgacgatc ttcatcgcaa tggcgatcgt gaaggcgatc
    10681 agcatcacca tcgccgcttc ggcgcgtccg gtcccgtgac cctcggccgc cgcgatctcg
    10741 gcatgcaccg tcgtcgcgac cagcgtcggc cagagatagc cggtcacggc caggacgagg
    10801 ccgccgccgc cccagatcca gaggaggtcg gcacgcgaga cggcgaggat gtcaccgaag
    10861 agataaccga tgagatcgag ccgctggccg gcgagcttcg aggccgcgac aaggccgcca
    10921 gccaatgccg catgcgagag gagcccgagc agcgtatcga tcggcagcac cttctggcgc
    10981 tcgagcgcga tcagcaggac gccgatcagc aaggatacga tgataatgcc gaccgtgaga
    11041 tcgatgccgg cgacgaggcc gagcgccacg cccagcagac ccgcatgcgc aaccgtcgag
    11101 ccgaaatagg ccatgcgccg ccagacgacg aggcagccga gcggcgccgc cacgatcgcg
    11161 aggccgacgc ccgcaagaat cgcgcggata atgaacggct cgagcatcga ggtgtggcgc
    11221 ccttcagagg atggtgtatc gaaagtccgt gggtgaggcg ttctacatgc tccttcgggg
    11281 gaaccgcaaa tctgctcgac gccagggcga cgacgggctc aacggtttgc tatgcacagc
    11341 tttcgacctg atcgagcgag gatcaatgag cagcgccgtt gccgagatcg accggcgcat
    11401 ccgcgagagc ttcgcgaagc aggggctcat gacaacactt ggcgccgagg tcacgcacgt
    11461 cggagtgggc gaggcggtga tcgagctccg cccgaggcct gcgatctcgc agcagcacgg
    11521 cttcgtccac gccggtgccg tcagtgccat cgcggacagc gcggcgggct atgccgcctt
    11581 gagcctgatg ccggcaaacc gtggcgtgct cacgacagaa ttcaagatca atctcctcgc
    11641 acccgctgcg ggcgagcgca tcatcgcgcg cggccgggtc gtgaaggctg ggcgggcgct
    11701 gacgctcgcc cagaccgatg tggtcgccct ggccgacggc cgggagaagc tgatcgcgct
    11761 cctcaccgcg acattcatgt cgatcgaggg gcgcgagggc atcgccgact gaacccaacc
    11821 tggagcctcc tcatgcagat cgaggtcgag acgacggtcg acgggacaat cgcagaagtc
    11881 tggcgcgcct acacgtcgcc cggggacatc atgcagtgga atgccgcatc cgacgactgg
    11941 cacacaccaa gcgcatcggt ggacctgcgc gaaggaggca agttcggctc ccgcatggaa
    12001 gccagggacg gcagccaagg cttcgatttc gagggtacct acacgaagat catcgagcat
    12061 cagctgatcg agtacgcctt cggcgaccga acggcaaaag tggaattcat ccccgaagcc
    12121 ggtggagtcc gagtgcgcgt cgccttcgat cccgatcaga cctatccgcc ggacattcag
    12181 cgcgccggat ggcaagcgat cctcgacaat tttgcgcggc atgtcggagc aaagcgccgc
    12241 cggcagtgat cctcggtccc cgggccaaag tggcgcattg cctcgcaacg gccgggcaca
    12301 gcatagcgag gtcggcgcac tgaggcgcga cggggcccaa tccagcatga ccgcaccgcg
    12361 gatcgatgac tatttcgtct gcgtcggcgc gcagaagtcg gggacgacct ggctggcgcg
    12421 cattctcgcg gaccatccgg agttgttcct cacaccggtg aaggagatcc actacttcga
    12481 tcatgtccgc ggcatcaccg ggcatctctc catggagaag cgccgctcac gctaccgcaa
    12541 gtaccaccag cgcctgtgga ccgagtgggg ccgcttcagc cgcaaccgcc agcattggcg
    12601 gtggtatcgc gactacatgc gccccgagat cggtgatcgc tggtatgcag acctcttccg
    12661 ccatcgcgac ggccgacgct ttgcgggcga ggtgacgccc gagtacgcca tcatcggccg
    12721 cgagggattc gagcacatcc ggcggcttgc gcccgccgcg cgcgtgctct tcatcatgcg
    12781 caatcccgtg gaacgggcct ggagccaggc gctccatcac gcccggtcgg aaaggatcga
    12841 cgcgggtcgc gcgagcctcg aagagctcaa acgcatcgtc gaccagccgc gttttcgcgc
    12901 gctcggcgac tacggcgcga ccctcgaggc catgagcgcg gtgttcccgg cagagcagtc
    12961 gctcgccatg ttctacgagg acatccacaa ggatcgccct gccgcgctcg aagcggtctg
    13021 ccggttcatc ggcattcgct ttgacccggc cctcctcggc gatacgggcc ggcgcttcaa
    13081 caagagccag gatgcaaagc ttcccgccga gctcagggtc cacctcgcca aatgctacaa
    13141 ggcgacggcg gaggatgtcc gccgtcgcct tggacgaatt cccgatgcct gggcgaagga
    13201 attcggattg ccttagttcg gagcgctcgg acagcgatgc ttaggcagcg atgcgagctg
    13261 cctcgccgac gctgatccac accttcgcct gctggccgat cgcctcgaca atcgtcaacc
    13321 cggcgacgtc gagctcgatc gactgaccgg cctcgacgat gatgtcgccc ggctcctggg
    13381 gctggctcat ccagagtgtt ccgtccaggg caaggagctt gaggctctgt ccctccggga
    13441 ggcgccagag cgcgcccgcc tcgagtgttg cagggccgga gacggtcaat agcggcaaca
    13501 ttggcttctc ctttacctcg ccggtgcctc gcagtcggcg tgtccggtct tcgtcggcga
    13561 cttgagtagc ctgaacggcc aggcgagcgc cgcaccgatg ctccgcgtca gcgacacgac
    13621 cgccgcgttg cgctcggcgt gagccttgag gcggatggcc gcgaggtcct cggccgacag
    13681 cgggtacttg tagggaaatt cattcgtgtt catgatcgtt ctccagggat cttgttgtcg
    13741 tgagccgaat atcggcctat tcaagcgatc cgacaaacga tcatgtctca cccatcactt
    13801 gagacaatct catgcgtcac gcgttaccgc cgctcaacgc actcagagcc ttcgaggctg
    13861 ccgcacggca tctgtcgctc accaaggcgg ccgaagagct gcacgtgacg ccggctgcgt
    13921 tgagccatca ggtgcgcggt ctcgaggagg cgctcggcgt ccgcctgttc cagcgtctgc
    13981 cgcgggcgct ggcgctcacc gatgccggcc gcaagcttct gcctggcctc tcgtcgggct
    14041 tttcccagat ccgccaggcc gtcgccctcg tccaggagac cggcggcggc cgcgtgctgg
    14101 tcgtgagcac accacccggc ttcacctcga aatggctcgc gccccggctg catcgcttct
    14161 tgacggccaa tccggatatc gaggtgcgcg tctcctcgac gctgaagaat gccgatttcg
    14221 agaccgacgg cgtcgatgtc gcggtccgga acttgcgcca ttcccagccg gtcgaccaga
    14281 gtctcttcag cgagcacctc gtggaccttg gcctcgtccc cgtctgctct ccggcgttcc
    14341 tcgctgccca tcccgagctc gtctcgccgt ccgacctcgc caaggtccca ctcgtccacg
    14401 acaatcagat ggccggtcgc ctggacaatc ccggatggcc ggaatggctc gccgccgccg
    14461 gagtagacgg ggtctccctc ggccatggcg tcaagttcaa cagcgccgat catgcgctcg
    14521 aagcggccct agaaggcgcg ggcgtcttgc tcgcgcacag cgtgctggcg agcgacgatc
    14581 tcaaggtcgg ccggctcgtc agtccgttcc cgatgacgat cgcgatgcgc cgcagcttcc
    14641 gcatcgtctg cccgatcggc cacgagacgc gagcgaacat ccgcacgttc cgggactgga
    14701 ttcatgccga gatggcggcg agcggcggcc gccgcttcga cgttcagcgc cgcgccggac
    14761 tgccggccgc cggaatgtag tcgagcacgc cgacgacgcg atacgtaacc tcagtccaca
    14821 cgatcgcaaa caccaccgct gccaccaaag tcgtgatcag gaacactttt ttcagtttcg
    14881 gcgccatagg tgcgctctcg ggcgtgccgg gtacgacgct gccggcctcc tcttgcgtgc
    14941 gtacgccgaa tggcagcacg gcaaagagca ctgtccacca gatgacgaaa tatagcgcaa
    15001 tgccgagtgc gatgctcatc gaccactccg ctgcagttta tccaaggccg ccggaccgca
    15061 tgacgaggaa ggcatccgtc ggcgcgaagc ggatctcgcc tttgacgagc cgaatgctgc
    15121 cgggccgatc gatccgcata ttgtggaccg aatgccagac gtggacgccg gcccggtcga
    15181 tctcggccgc gatggcgagc gcctcgtcac cgctagcggc cgcgagccgg cgcatctcgt
    15241 cggcgacatc ggcaaacgcc ggccgatgcg tgaaggtcga agccatggtg ccgtcgtcgt
    15301 cggctcgcag gctgccgagc gtcgcgagct cgcgctcgcc ggaccgcagc accgcgccgc
    15361 cggtatcgtt gctccgtcgc gtgaccacgg ctcttcgcat cagtccctcg c
//
LOCUS       DAORNH010000076        15308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2940_length_15308_cov_2.610109, whole genome shotgun sequence.
ACCESSION   DAORNH010000076 DAORNH010000000
VERSION     DAORNH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2940_length_15308_cov_2.610109"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK264_09775"
     CDS             <1..714
                     /locus_tag="PK264_09775"
                     /inference="COORDINATES: protein motif:HMM:NF037032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76214.1"
                     /translation="IDRRTISLFIEAGHSAILSTAAPLPRFHAVLARSSGRMQAASLA
                     VLRQLELLGAVSLNPADALSLLAEPIRRAQELARAGLAAPALAVSFTGAGRKGGDDRM
                     VAAGGELSLPAQAGRKHLRFLVIAGAARAVMLRSDDALADDETAQAWLADEVSRYAEA
                     GHAAKAAAQAFRLGVAAVDVDLSSATPLVTDISPAPTLALFERVAHIDAAGALIEAIE
                     VAVAADVPLRSRASPSGGR"
     gene            complement(680..2206)
                     /gene="gatB"
                     /locus_tag="PK264_09780"
     CDS             complement(680..2206)
                     /gene="gatB"
                     /locus_tag="PK264_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRD76215.1"
                     /translation="MQDKSRAGGKSGKAKPTHTIDGATGPWEIVIGMEIHAQVATKAK
                     LFSGASTEFGAAPNAHVSLVDAAMPGMLPVINRACVDQAVRTGLGLDAAINLKSVFDR
                     KNYFYPDLPQGYQISQYKQPVVGEGRVSIDADGETIEVGIERLHLEQDAGKSIHDQHP
                     DYSYVDLNRSGVALMEIVSKPHLRSSKQAQAYVSKLRTILRYLGTCDGDMEKGNLRAD
                     VNVSVCKPGAYEKFRASGDFKHLGTRCEIKNVNSIRFIGQAIEVEARRQIGIIEDGGT
                     IDQETRLFDSVKGETRSMRSKEEAHDYRYFPDPDLLPLELTRDEVERLKAGLPELPDA
                     KQARFVQAYGLSAYDAAVLVAERESADFFETVAKGRDGKLAANWVINELFGRLNKEGL
                     KIGESPVGATALGEIVGLIEAGTISGKIGKDLFEIVWTEGGKPAEIVEKRGLRQVTDT
                     GAIEAAIDAVIKANPDKVAAVKAKPTMLGWFVGQVMKASGGKANPQAVNDLLKAKLGI
                     "
     gene            complement(2289..3092)
                     /locus_tag="PK264_09785"
     CDS             complement(2289..3092)
                     /locus_tag="PK264_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD76216.1"
                     /translation="MTDQKSLVQSQFGASAAAYSTSKVHAKGASLGRLVELVKPEKHW
                     RALDVATGAGHTAAAFAPHVASVVASDITPEMLGEAQKLAKAKGFANMTTAAADAEAL
                     PFPDKSFELVTCRIAPHHFPNVKAFVGEVARVLTPGGTFALVDNISPDNLSTPGFSAA
                     ELQEAAAAYNAFEKLRDPSHGRALAISEWLGLIEAADLNVAHQERLPKDMEFQDWAVR
                     MGCTRETIATLRGMLTGASPALGAFLKPRSAGEDIHFTLDEALIIARKG"
     gene            complement(3089..4408)
                     /locus_tag="PK264_09790"
     CDS             complement(3089..4408)
                     /locus_tag="PK264_09790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76217.1"
                     /translation="MTQHLKPVIALILSAAILLMGNGLLGLLGPIRADGAGFTRLDIG
                     IMGSFHFAGLMAGCILWPRVVAKVGHIRAFAALTAMAAITPLCQAIWTTPVVWWGLRA
                     LNGLCFAGLFMVIETWLTGASTPETRGRVLGVYTMVNLTVVTVGMQLIGVGDPKSFEL
                     FSLVAILYALAAVPVALAPQTPPSPPREAKLRLGWLYSVSPAAVMGCFFTGIANSSFW
                     SLSPLYAQGAGLSIKEAAVFVTSAVLGGAASQWPVGRLSDRLGRRAVAIVVCSVAALA
                     GVLLFTMADKPPRALLLSLAALYGTMAFTVYTLCVAHANDLVHKKRAVEVSSGLLLTF
                     SGGAILGPLAASYLMHIFGHGGLFLHSAIAHGAIAAVMLLRASQRPTLPIARREPFVP
                     VPKTTPAVFDLDPRGEGEDKDAPTPIDAKLPPANVARPTPQPEGARP"
     gene            4593..>5301
                     /locus_tag="PK264_09795"
     CDS             4593..>5301
                     /locus_tag="PK264_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD76218.1"
                     /translation="MTYRAPVDDIMSALKTAAGLDDLIARGVVKVDEETLRAVVEEAG
                     KFGSEVLDPLNWSGDRTGSKLVDGKVVTPAGWTEAYKAFVEGGWGALPCPEAFGGQGL
                     PEVASMAACEIWNASNLSFGLCPLLTQGAIAALIAQGSDALKAQYLPKMVSGEWTGTM
                     NLTEPHAGSDLGHLRSRAERMSDGSYRIFGTKIFITYGDHEMTENIIHLVLARLSDAP
                     AGTRGISLFLVPKHLLNA"
     assembly_gap    5302..5367
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5368..6355
                     /locus_tag="PK264_09800"
     CDS             <5368..6355
                     /locus_tag="PK264_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRD76219.1"
                     /translation="KYGEGGEGAIGYLVGEENRGLNAMFIMMNEARLAVGVQGVALAE
                     RATQRAIAYAKERRQGRSAKGKAGEMSPIIEHADIRRTLMIMKALTQAARAICLATAR
                     EVDISHHAADAAERQTAANRTALLTPIAKAFSTDIAIEVTSMGVQIHGGMGFVEETGA
                     AQHYRDARILPIYEGTNGIQALDLVTRKLPIEGGKVAAAYIGELAKTVEEVRASNRPE
                     FGRMAERLGAAVESLGAASRWVGKALASNQEAALAGAQPYLRLFGLAAGGVYLARAAL
                     DATRTSGGASAQTVAIARFFAENLATAAPGLADTVMTGADGLLAVEPDALAG"
     gene            complement(6368..6844)
                     /gene="dut"
                     /locus_tag="PK264_09805"
     CDS             complement(6368..6844)
                     /gene="dut"
                     /locus_tag="PK264_09805"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRD76220.1"
                     /translation="MTAHRATVEIKILDTRLREWGLPRYQSDGAAAIDLLACLDEPLL
                     LAPQAPAVLIPSGIAVSMGDANLAALIVPRSGLGHKQGLVLGNLMGLIDADYQGPIMI
                     SAWNRSAPGTAPIRIAPGERIAQMLFVPIVRPAFEVVDAFASATRRGAGGFGSTGR"
     assembly_gap    6950..6959
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7108..8400)
                     /locus_tag="PK264_09810"
     CDS             complement(7108..8400)
                     /locus_tag="PK264_09810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRD76221.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLV
                     IDGKTYKLSLLPSGLVRKGKLSVIGNGVVLDPWHLVKEIADLKAQGVDVNPDVLRIAE
                     NATLILPLHSDLDKLREEAAGGQKIGTTGRGIGPAYEDKVGRRAIRVQDLKNLGTLEP
                     KIDRLLVHHNALRRGLGRPEIDKGKLMADLVEISPKLLPYMDTVWDLLDKARRQGKRI
                     LFEGAQGALLDVDHGTYPFVTSSNTVAAQAATGSGLGPKSIGYVLGLAKAYTTRVGSG
                     PFPTELNDEVGRMLGERGREFGTVTGRARRCGWFDAVLVRQVCKVSGIDGIALTKLDV
                     LDGLKEIRVCVGYRLDGERISKLPAGQNAQARVEPIYEVLSGWSGSTKGAKKWSELPA
                     EAVKYVRHIEELIECPVTLLSTSPERVDTIMVKDPFAD"
     gene            complement(8517..9341)
                     /locus_tag="PK264_09815"
     CDS             complement(8517..9341)
                     /locus_tag="PK264_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76222.1"
                     /translation="MPQANQIVLDSVTHLTDAHRGKAALCASHGGHYAAYYGATKAVS
                     ALILNDAGVGRERVGFSGSIYLERLGLPAATISSQSARIGDGRDGHANGIISFVNAPA
                     AKLKLKVGMRCREALDMLATAGLPPSPPPPAEDEHRTEIVDAGRGKTRVIAMDSISLV
                     ATTDRQNIVVTGSHGGLLGGRKETAIKFPVAGVVCNDAGFGRDNAGMSRLPVLDALGI
                     PAACVSNFSARIGDGMSTYRDGYVSALNEAAKKAGGLIGQSCRELVALIVASAPSP"
     gene            complement(9856..10110)
                     /locus_tag="PK264_09820"
     CDS             complement(9856..10110)
                     /locus_tag="PK264_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HRD76223.1"
                     /translation="MWPRPAASLPQFNEVGVPAFDGTAWAGLLAPVDTPRDIVDKLAS
                     AAAKAARSGPDREFTSRIGATAVGSTPAEFSTFLAAGRAR"
     gene            complement(10153..10935)
                     /locus_tag="PK264_09825"
     CDS             complement(10153..10935)
                     /locus_tag="PK264_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRD76224.1"
                     /translation="MNMRTRAAALGAALCLSLLVSYLPQSRAQGGVSSRNATVVAYAT
                     GQFRQTGPKAWVEVGNGGGTFNFTETGRNDTSVYLSDASRGIRIELNVSSRQIFLAHG
                     GGPKGLLYRVTSVTADPVGPGTPPPPPIVGGGNDVAAVLALVNRHRSDGAVCGGQMMR
                     PVPPLKLNAALTTAAAEWSLAMQRAGKLSHDRMGERLAAACGGAREGGENVAAGSPTP
                     ALVLQNWMQSPGHCRNIMSPSMTDIGIGRAGNFWTQLFAKDC"
     gene            11170..12726
                     /locus_tag="PK264_09830"
     CDS             11170..12726
                     /locus_tag="PK264_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRD76225.1"
                     /translation="MIGPFRLDTDARVLFRDTAPLPLSQRAVVLLCVLVEHHGHLVSK
                     NSLVQAAWDGRAVEESNLTVQIASLRRVLGEVPGGERWVETLPRHGYRFVGPAPIRER
                     VQVSSREAPAVAPTPSAKPPVEKPSIVVLPFQNLSGNPTQDYFGEGFAEDITTALSRL
                     KWLVVAARDSSDAASTTAADHQRLARDRGVRYVLNGSTRLAGDQLRVTCQVIDAENWQ
                     TILSRKFDRRLGDVFAVQDEITESLVAVIEPSLLDAEEMRARQRPLVRLDCWGLIARA
                     VGLVHRFEREANLTAQGHLQRAIALDPSSARAQATLAWALYWAFHCSWVDNRAGCAEQ
                     AMAHAETALRLDPQEPWARAVYGFNLSSSGKHAAAIDELRRVLELQPSFALGRMLLGW
                     ALLRRGDFIAAIGETQRALLLSPGDRFTAVYQHAHGLALLAARRFEEALPFLRSAAAT
                     YVEYMGHVNGLISCCGHLGLVDEARHLIEFRRACLGREFTIAFARARLSAFAHCDVFL
                     EGLAKAGVPD"
     gene            13250..>13842
                     /gene="lysK"
                     /locus_tag="PK264_09835"
     CDS             13250..>13842
                     /gene="lysK"
                     /locus_tag="PK264_09835"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418788.1"
                     /note="class I; LysRS1; catalyzes a two-step reaction,
                     first charging a lysine molecule by linking its carboxyl
                     group to the alpha-phosphate of ATP, followed by transfer
                     of the aminoacyl-adenylate to its tRNA; in Methanosarcina
                     barkeri this enzyme charges both tRNA molecules for lysine
                     that exist in this organism (but the tRNALysUUU very
                     poorly) and in the presence of LysRS2 can charge tRNAPyl
                     with lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRD76226.1"
                     /translation="MSTSATAALAAEARAWPFEEARRLIQRLDKLKGGRDKTVIFETG
                     YGPSGLPHIGTFGEVARTTMIRRAFEVISEIPTRLLCFSDDMDGFRKVPDNVPSKDML
                     AAHLQKPLSRVPDPFCNEHGSYAAHNNAVLRAFLDRFGFQYEFLSATEEYTSGRMDEA
                     LLRMLAGYDKVMDIILPTLGPERRATYSPFLPVCPRTG"
     assembly_gap    13843..13890
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13891..14847
                     /gene="lysK"
                     /locus_tag="PK264_09840"
     CDS             <13891..14847
                     /gene="lysK"
                     /locus_tag="PK264_09840"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596651.1"
                     /note="class I; LysRS1; catalyzes a two-step reaction,
                     first charging a lysine molecule by linking its carboxyl
                     group to the alpha-phosphate of ATP, followed by transfer
                     of the aminoacyl-adenylate to its tRNA; in Methanosarcina
                     barkeri this enzyme charges both tRNA molecules for lysine
                     that exist in this organism (but the tRNALysUUU very
                     poorly) and in the presence of LysRS2 can charge tRNAPyl
                     with lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRD76227.1"
                     /translation="AYVDPEGGQKVETKVTGGAVKCQWKADWALRWVALGVDYEMAGK
                     DLIDSVMLSSKICRALGGTPPEGFNYELFLDENGEKISKSRGNGLTADEWLTYAPPES
                     LSLFMYQKPKTAKRLYFDQIPKAVDEYLGFVSAFPGEPGAKQLDNPAWHIHGGRPPLP
                     ELPVSFALMINLVSASNAANKSTLWGFIGRYAPGASPEKNPILDRLAGYAIAYYRDFV
                     LPTKTYRAPTASERKALGALDAELAKASPDASAEALQTIVYEVGKANGYAESLRDWFK
                     AIYEVLLGQTQGPRFGSFIALYGIAETRALIAKGLAGKLGKA"
     gene            15110..>15308
                     /locus_tag="PK264_09845"
     CDS             15110..>15308
                     /locus_tag="PK264_09845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD76228.1"
                     /translation="MEQTTFAGLGLSSKVLAAIDAVGYTTPTPIQAEAIPVAVTGRDV
                     LGIAQTGTGKTASYVLPMLSRL"
BASE COUNT         2464 a         4980 c         5158 g         2582 t
ORIGIN      
        1 attgaccggc gcacgatctc gcttttcatc gaggccgggc actcggcaat cctctcgacg
       61 gcggcgccct tgccgaggtt ccacgctgtt ctcgcgcgat cttcgggccg catgcaggcc
      121 gcctcgctcg cggtcctgcg ccagctcgag ctgctcggtg cggtctcgct caatccggcc
      181 gatgccttgt cgctgctcgc cgagccgatc cggcgcgcgc aggagctcgc acgggccgga
      241 cttgcggcgc cagcgttggc ggtctcattc acgggtgccg gccggaaggg tggcgatgac
      301 cgcatggtcg cagcaggcgg ggaactgtct ctgccggcgc aggctggccg gaagcacttg
      361 cgcttcctgg tgatcgcagg cgccgcgcgc gccgtcatgc tgcgctcgga tgatgcgctc
      421 gcggacgacg agacggccca ggcctggctc gccgacgagg tgtcgcgata cgcggaggcg
      481 ggccatgctg ccaaggcggc ggctcaagcg ttccgccttg gggttgccgc agtcgatgtg
      541 gacctctctt cggcgacgcc gctcgtcacg gacatttcgc cggcccccac tctcgcgctg
      601 ttcgagcgcg tggcgcacat cgatgcagcc ggcgccctga tcgaggccat cgaagtcgcc
      661 gtcgcggcgg acgtgcccct cagatcccga gcttcgcctt caggaggtcg ttgacggcct
      721 gcgggttcgc cttgccgccc gaggccttca tcacctggcc gacgaaccag ccgagcatgg
      781 tcggcttcgc cttgacggct gcgaccttgt ccgggttcgc cttgatgacg gcgtcgatcg
      841 cggcctcgat ggcgccggtg tcggtcacct gccgcagccc gcgcttctcg acgatctccg
      901 cgggtttgcc gccttcggtc cagacgatct cgaagaggtc cttcccgatc ttgccgctga
      961 tggtgccggc ttcgatcagg ccgacgatct cgccgagcgc cgtcgcgccg accgggctct
     1021 cgccgatctt gaggccctcc ttgttgaggc ggccgaagag ctcgttgatc acccagttgg
     1081 cagcgagctt gccgtcgcgg cccttggcga ccgtctcgaa gaaatcggcg ctctcgcgct
     1141 cggcaacgag caccgcggcg tcgtaagcgg agaggccata ggcctgcacg aagcgcgcct
     1201 gcttggcatc gggaagctcg ggaagccccg ccttcagccg ttcgacctcg tccctggtga
     1261 gctcgagggg aagcaggtcc ggatcgggga aatagcgata gtcgtgcgcc tcctccttgg
     1321 aacgcatcga gcgcgtctcg cctttcacgg aatcgaacaa ccgcgtctcc tggtcgattg
     1381 tcccgccgtc ctcgatgatg ccgatctgtc ggcgcgcctc cacctcgatc gcctggccga
     1441 tgaagcggat cgagttgacg tttttgattt cacagcgcgt gccgaggtgc ttgaagtcgc
     1501 cgctcgcgcg gaacttctca taggcgcccg gcttgcagac cgaaacgttg acgtcggcgc
     1561 gcaggttgcc cttctccatg tcgccgtcgc aggtgccgag atagcgcagg atcgtgcgca
     1621 gcttcgagac gtaggcctgc gcctgcttcg aagagcgcag gtgcggcttg gaaacgatct
     1681 ccatcagtgc cacgcccgac cggttgaggt cgacgtagct gtagtcggga tgctggtcgt
     1741 ggatggattt ccccgcatcc tgctcgagat ggaggcgctc gataccgacc tcgatcgtct
     1801 caccgtcggc gtcgatcgag acacggccct cgccgacgac gggctgcttg tactggctga
     1861 tctggtagcc ctgcggcagg tccggataga agtagttctt gcggtcgaag acgcttttca
     1921 ggttgatcgc ggcatcgagg ccgaggcccg tgcggaccgc ctgatcgacg caggcgcgat
     1981 tgatcaccgg cagcatgccg ggcatcgccg catcgacgag cgagacatgc gcgttgggtg
     2041 cggccccgaa ctcggtcgag gcgcccgaga agagcttcgc cttcgtcgcg acctgggcgt
     2101 ggatctccat gccgatcacg atctcccacg ggcccgttgc gccatcgatg gtatgcgtcg
     2161 gcttggcctt gccggacttg ccaccggccc tgctcttgtc ctgcatctct ggatctcgtc
     2221 gctcacatgc gaatgggtcg acgcctcata gcaaaccccg gaccaggatg gccagagccg
     2281 cagaaggttc agcccttccg cgcgatgatc agcgcctcgt cgagcgtgaa gtggatgtct
     2341 tcgcccgcgg agcgtggctt gaggaaggcg ccaagcgccg gcgacgcgcc ggtcagcatg
     2401 ccgcgcagcg tcgcgatcgt ctcgcgcgtg cagcccatgc gtacggccca atcctggaac
     2461 tccatgtcct tcggcagccg ctcctgatgg gcgacgttca ggtcggccgc ttcgatcagg
     2521 ccgagccatt cgctgatggc gagcgcgcgt ccgtgagagg gatcgcggag tttctcgaag
     2581 gcattgtagg ccgccgccgc ttcctgaagc tcggcggcgg agaacccggg cgtcgagagg
     2641 ttgtccggcg agatgttgtc gacgagcgcg aaagtgccgc cgggggtgag cacacgcgcg
     2701 acctcgccga cgaacgcttt gacgttcgga aagtggtgcg gcgcgatgcg gcaggtcacg
     2761 agttcgaagc tcttgtccgg aaatggcagc gcctccgcat cggccgcggc cgtcgtcatg
     2821 ttcgcgaagc ccttggcctt ggcgagcttt tgcgcctcgc cgagcatctc tggcgtgatg
     2881 tcgctcgcca ccacgctcgc gacgtgcggg gcgaaggccg cggccgtatg ccctgcgccc
     2941 gtcgcgacgt cgagcgcgcg ccagtgcttt tctggcttta cgagctccac gaggcggccg
     3001 agggaggcgc ccttggcgtg aaccttcgag gtcgagtagg cggcagcgct ggcgccgaac
     3061 tgcgattgga ccagcgattt ctggtcggtc atgggcgggc tccctcgggc tgcggcgtgg
     3121 gccgcgcgac atttgctggc ggcagcttag catcgatcgg cgtcggcgcg tccttgtcct
     3181 cgccttcgcc gcgcggatcg aggtcgaaca cggcgggcgt cgtcttcggc actggcacga
     3241 acggttcgcg ccgcgcgatc ggcagcgtcg ggcgctggct cgcgcgcagc agcatcacgg
     3301 ccgcaatggc gccgtgcgcg atcgcggagt gcaggaagag cccgccgtgg ccgaagatgt
     3361 gcatcaggta ggaggcggcg agcggcccga gaatggcgcc accggaaaac gtcagcaaga
     3421 gcccgctcga cacttcgacg gcgcgcttct tgtgcacgag gtcgttcgcg tgggcgacgc
     3481 acagcgtgta gaccgtgaac gccatcgtgc cgtagagcgc tgcgagcgat aggagcagcg
     3541 cacgcggcgg cttgtcggcc atggtgaaga ggagaacgcc cgcaagggcc gcgaccgagc
     3601 agacgacgat ggcaacggcc cgccgcccga gccggtcaga cagccgaccg accggccact
     3661 ggctggccgc gcccccgagc acggcggatg tcacgaatac ggcagcctcc ttgatcgaga
     3721 ggccggcgcc ctgcgcatag agcggcgaca gcgaccagaa gctcgagttc gcgatgcccg
     3781 tgaagaagca gcccatgacg gcagccggcg acaccgaata gagccagccg agccgcaact
     3841 tggcttcgcg aggtggcgag ggcggcgtct gcggcgcgag cgccacggga acggccgcca
     3901 acgcatagag gattgcgacc agcgagaaga gctcgaagct tttcggatcg ccgaccccga
     3961 tcagctgcat gccgaccgtg accacggtga ggttcaccat cgtgtagacg ccgagcacgc
     4021 gcccgcgcgt ctcgggcgtc gaggcgcccg tcagccacgt ctcgatcacc atgaagaggc
     4081 cggcgaagca gaggccattg agggcacgca gcccccacca cacgacgggc gtcgtccaga
     4141 tcgcctggca gagcggtgtg atcgcggcca tggcggtgag tgccgcgaag gcgcggatgt
     4201 ggccgacctt ggcgacgacg cgcggccaga ggatgcagcc cgccatcagg cccgcgaagt
     4261 gaaaggagcc catgatgccg atgtcaagcc gcgtgaagcc tgctccatcg gcccggatgg
     4321 gtccgaggag accgaggagc ccgttcccca tgagcaggat cgccgcgctg aggatcagcg
     4381 cgatcacggg tttcaagtgc tgcgtcatgt gtcctctgcc gccccgtgtg tcagctgtgc
     4441 gctggctctc gaggtgagat acaacgcgcc gtgcgcgcgg gtccaccttc tgagcgcttc
     4501 catgaaccgc gaatttgtgc ttcggagacg cagtatcggc ctgaatccgg tatgcagtcg
     4561 tgtgccggca gaccagcgca agcgaggcgt ccatgaccta tcgtgccccc gtcgacgaca
     4621 tcatgagtgc gctgaagacg gcggcaggcc tcgatgacct catcgcccgc ggcgtcgtga
     4681 aggtcgacga ggagacgctg cgcgccgtcg tcgaggaggc cggcaagttc ggctccgagg
     4741 tgctcgaccc gctcaactgg tcgggcgacc gcaccggctc gaagctcgtc gacggcaagg
     4801 tcgtgacgcc ggctgggtgg acggaggctt acaaagcgtt cgtcgagggc ggctggggag
     4861 cactcccctg tcccgaggcc ttcggcggcc aaggcctgcc ggaagttgcc tccatggcgg
     4921 cctgcgagat ctggaacgcg tcgaacctct cgttcggcct ctgcccgctg ctgacgcagg
     4981 gcgcgatcgc ggcgctgatt gcccagggct ccgacgcatt gaaggcgcag tacctgccga
     5041 agatggtgtc gggcgagtgg accggcacca tgaacctgac cgagccgcat gcgggctccg
     5101 acctcgggca cctgcgctcg cgcgccgagc gcatgtcgga cggcagctac cgcatcttcg
     5161 gcacgaagat cttcatcacc tacggcgacc acgagatgac ggagaacatc atccatctcg
     5221 tgctcgcgcg actctccgat gcacccgccg gcacgcgcgg catctcgctg ttcctggtgc
     5281 cgaagcacct gctcaacgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnngaa gtacggcgaa ggcggcgagg gcgccatcgg
     5401 ctatctggtc ggcgaagaga accgcggcct caatgccatg ttcatcatga tgaacgaggc
     5461 gcggctcgcc gtcggcgtgc agggtgtggc gctcgccgag cgggcgacgc agcgtgccat
     5521 cgcctacgcc aaggagcgtc gccagggccg ctcggccaag ggcaaggccg gcgagatgag
     5581 cccgatcatc gagcacgccg acattcgccg cacgctcatg atcatgaagg cgctgacaca
     5641 ggcggcgcgc gccatctgcc tcgcgacggc tcgcgaggtc gacatctcgc accatgcagc
     5701 cgacgcggcc gagcgtcaga ccgccgccaa ccgcaccgcg ctgctgacac cgatcgccaa
     5761 ggccttctcg accgacatcg ccatcgaggt gacctcgatg ggcgtgcaga ttcacggcgg
     5821 catgggcttc gtcgaggaga cgggcgcggc gcagcactac cgtgatgcgc gcatcctgcc
     5881 gatctacgaa ggcaccaacg gcatccaggc gctcgacctc gtcacgcgga agctgccgat
     5941 cgagggcggc aaggttgcgg ccgcctacat cggtgaactc gcaaagaccg tcgaggaggt
     6001 gcgcgcctcg aaccggcccg agttcggccg catggcggag cggctcggcg cggccgtcga
     6061 gagcctaggt gctgcctcgc gctgggtcgg caaggcgctc gcctcgaacc aggaggcggc
     6121 gctcgccggc gcgcagcctt atctccgctt gttcgggctt gcagcgggcg gcgtctatct
     6181 cgctcgcgcc gcgcttgatg cgacgcgcac cagcggaggc gcctccgcgc agacggtggc
     6241 gatcgcgcgc ttctttgccg agaacctcgc gaccgcggcg ccgggcctcg ccgacacggt
     6301 catgacggga gcagacggtc tcctcgccgt cgagcctgat gcacttgcgg gctgaggcag
     6361 ccttgcctca gcgcccggtc gagccgaagc cgccggcgcc gcggcgggtc gcgctcgcga
     6421 acgcgtcgac gacttcgaat gccggccgga cgatcggcac gaacagcatc tgcgcgatcc
     6481 gctcacccgg tgcgatgcgg atcggcgccg tgccaggcgc actccggttc caggcgctga
     6541 tcatgatcgg gccttggtag tccgcatcga tgaggcccat cagattgccg agcaccaagc
     6601 cttgtttgtg cccgaggccg gagcgcggca cgatcagcgc ggcgaggttc gcatccccca
     6661 tgctcacagc aatgcccgag gggatcagga cggccggtgc ctgcggcgcg aggagcagcg
     6721 gctcatcgag gcaggcgagg aggtcgatcg ccgccgcgcc gtcgctctgg tagcgcggca
     6781 ggccccactc gcggagccgc gtgtcgagga tcttgatttc gaccgtggct cggtgcgccg
     6841 tcatcgtcgg gcctcatcag cttgccgacc tggctctgct aggcgcgatt cctcccgtgc
     6901 cgctagcgtc ccctcccccc ttgcggggga gggacagggt ggggggtgtn nnnnnnnnnc
     6961 ggccgaaggc agccagtgcg gcgagcagcc ctggatcgtc gtgcaccacc cccatccccg
     7021 acccttcccc cgtcaagggg gaagggagtg aagtactgac cgaagaggac aacatcatcg
     7081 agcgccccgg ttccaacgaa cgcccgttca atccgcgaac gggtccttga ccatgattgt
     7141 gtcgacgcgc tccggactcg tcgagagcag cgtcacaggg cactcgatca gctcctcgat
     7201 gtggcgcacg tacttgacgg cctcggccgg cagctcgctc cacttcttgg cacccttggt
     7261 cgagcccgac cagccgctca gcacctcgta gatcggctcg acgcgcgcct gcgcgttctg
     7321 cccggccggc agcttcgaga tgcgctcgcc gtcgagccgg tagccgacgc agacgcggat
     7381 ctccttgagc ccgtcgagca cgtcgagctt ggtcagcgcg atgccgtcga tgcccgagac
     7441 cttgcacacc tggcggacca gcacggcgtc gaaccagccg cagcggcgcg cgcgaccggt
     7501 cacggtcccg aactcgcgcc ccctttcgcc gagcatgcgg ccgacctcgt cgttgagctc
     7561 ggtcgggaac ggcccagagc cgacacgcgt cgtgtaggcc ttggcgaggc cgagcacgta
     7621 gccgatcgat ttggggccaa gccccgagcc cgtcgccgcc tgcgccgcga ccgtatttga
     7681 cgacgtgacg aacgggtagg tgccgtggtc gacgtcgaga agtgcgccct gcgcgccctc
     7741 gaacaggatg cgctttcctt gccggcgcgc cttgtcgagg agatcccaga cggtgtccat
     7801 gtaggggagc agcttcggcg agatctccac gagatcggcc atcagcttgc ccttgtcgat
     7861 ctcgggccgg ccgaggcccc ggcgcaatgc gttgtgatgg acgagcaggc gatcaatctt
     7921 tggctcgagg gtcccgaggt tcttcaggtc ctgcacgcgg atcgcgcggc ggccgacctt
     7981 gtcctcgtag gccgggccga tgccgcgacc cgtggtgccg atcttctgac cgcccgcggc
     8041 ctcctcgcgc aacttgtcga ggtcgctatg tagaggaagg atcagcgtcg cattctcggc
     8101 gatgcgcagc acgtccggat tgacgtcgac gccctgcgcc ttgaggtcgg cgatctcctt
     8161 cacgagatgc caggggtcga ggacgacgcc gttgccgatg acgctgagct tgcccttgcg
     8221 cacgaggccg gaggggagga gggacagctt gtaggtcttg ccgtcgatga cgagggtgtg
     8281 gcccgcgttg tgcccgccct ggaagcgcac gacgatgtcc gcgcgctcgg acagccaatc
     8341 gacgattttt cctttgcctt cgtcgcccca ctgggcgccg acaacgacga cgttggccat
     8401 ccggccctcc tcacgtgcag actgatgcac gagagcgatg ggcgccccct gtgcgacggt
     8461 cggttacgct ttcgtgacac gctcggcaag cggaaacgac gtagccgcct ctgcaactat
     8521 gggctcggag cggaggcaac gatcagcgcg acgagctcgc ggcacgattg cccgatgagg
     8581 ccgccggcct tcttcgcggc ctcgttgagt gcggagacgt agccgtcgcg ataggtcgac
     8641 atgccgtcac cgatgcgggc cgagaagttc gagacgcagg cggcggggat gccgagggca
     8701 tcgagcacgg gaagccgcga catgccggcg ttgtcgcgcc cgaagcccgc gtcgttgcac
     8761 acgacaccgg caacagggaa cttgatcgcc gtttccttcc gcccgccgag cagtcctccg
     8821 tgcgagcccg tcaccacgat gttctgccgg tccgtggtcg cgacgagcga aatcgaatcc
     8881 attgcgatga cgcgcgtctt cccgcgcccc gcatcgacga tctcggtgcg gtgttcgtcc
     8941 tcggcaggag gtggtggtga gggcggcagt ccggccgttg ccagcatgtc gagcgcctcg
     9001 cggcagcgca tgccgacctt gagcttgagc ttggcggccg gcgcattgac gaacgagatg
     9061 atgccgttgg cgtgaccgtc acggccatcg ccgatgcgtg ccgactggct cgagatggtc
     9121 gccgccggaa ggccgagccg ttccagatag attgagcccg agaagccgac gcgctcgcga
     9181 ccgacgccgg catcattgag tatcagcgct gagacggcct tcgtggctcc ataataggcc
     9241 gcgtagtggc cgccgtgcga ggcgcacagt gccgccttgc cgcgatgcgc gtccgtcaga
     9301 tgcgtgacac tgtcgaggac gatctggttc gcttgtggca tgtgggcact ccggaagcgg
     9361 gccgccgcgc gagaaacctc gcaccaatcc gagtgggcag caagcgcgga caatgtggcg
     9421 aggtacggcc ggagcacatc aattctggcg cgctgttgcg ctcggcggct gcggcctggt
     9481 gtggtgacat tgcaggatgg gaggcgcgcg atcgctgagt gccatgacgg gacatggcgc
     9541 caaccctcaa tatgtcgtcc tcgggcttgt cccgaggtcc catcgggcgg cgtcctcagc
     9601 tgagtggttc ttgacccaaa gggaaactga gcaagtacag caattgagtg ctctattgac
     9661 cgcgtcatcg cggatggaca tccgcagtat tgatgccgca gtcgtgaggc tgatcgaggt
     9721 cgttggcgtg gacgaggtcg cttcggaccg tcgtacaggc gcttcccgga tcttgtgacg
     9781 gacatgcgcc ggaagcggcg aacgcgacgt cgagcccaag accatgttga tgttcgcgtc
     9841 ggcgatgact ttcgtctatc gcgcgcgtcc cgcggcgaga aatgtcgaga actcggctgg
     9901 cgtgctgccg acggctgttg cgccgatcct cgatgtgaac tcgcgatccg ggccgcttcg
     9961 cgcggccttt gcggcagcgc tggcgagctt gtcgacgatg tccctgggcg tatcgaccgg
    10021 cgccagcagt ccggcccatg cagtgccgtc gaacgccggc acgccgacct cgttgaactg
    10081 cggtagggac gctgcaggcc gaggccacaa ggcagcgtcc ctagaacaat ccgattggcg
    10141 aacgcagtta gatcagcagt ccttggcgaa caactgcgtc cagaaatttc cagcgcggcc
    10201 gatgccgatg tcggtcatcg aagggctcat gatgttcctg cagtgacccg ggctctgcat
    10261 ccagttttgc agcaccaacg ccggcgtggg cgaacctgcg gcgacattct cgcccccttc
    10321 ccgcgcgccg ccacaggctg cagcgaggcg ctctcccatg cggtcatggc tgagtttgcc
    10381 tgcccgctgc atggcaaggc tccattccgc ggcggcagtc gtcagcgctg cgttcagctt
    10441 cagcggcggt acggggcgca tcatttgacc gccgcaaacg gccccatcgc tgcgatggcg
    10501 gttgacgagg gcgaggacgg ccgcgacgtc gttgccaccg ccgacgatag gtggaggcgg
    10561 cggcgtgccg gggccgacag ggtccgccgt aacgctggtg acacgataga gcaggccttt
    10621 gggaccgcca ccgtgcgcca ggaaaatctg gcgcgagctc acatttagtt cgatgcggat
    10681 gccgcgcgac gcgtccgaga gatagacaga ggtatcgtta cgaccggtct cggtgaaatt
    10741 gaacgtgccg ccgccattgc ctacctcgac ccacgccttg ggaccggtct gtcgaaattg
    10801 accggtcgca taagcgacga cggtggcatt cctgctgctg acaccgcctt gtgcccgcga
    10861 ttgcggcagg tacgagacaa gcaggctcag gcagagtgcg gcacccaaag ccgccgcgcg
    10921 tgttcgcatg ttcatcgatg aatcctctga agcggagacc aagagcgggg cacaagtcgg
    10981 cccgttcgcg gcataggctc ccgccgcgaa ggttctcgtg ctagcgtatc tctgtcgccg
    11041 ttccttcagt gcgtggccgg cgacgtgtga tcagggtgca ggcgacgcga acgaaagtca
    11101 gaaaagagct ccgaaaaatt tccgaattgt ccaacgccgc gaattatact ggtgcagtca
    11161 atgagttgca tgatcggccc cttccgcctc gataccgacg cgcgggtcct gtttagagac
    11221 accgcgccgc tgccgctgag ccagcgcgcc gtcgtcctgc tgtgcgtctt ggtcgagcat
    11281 cacggtcatc tggtgtcgaa gaacagcttg gtgcaggcgg cctgggacgg gcgggccgtc
    11341 gaggaaagca acctgactgt ccagatcgcc tcgcttcgcc gcgtgctcgg ggaagtgccg
    11401 ggtggcgaac gatgggtcga gacattgccg cgccacggct atcggttcgt cgggccggcc
    11461 cccatccgag agcgcgtaca ggtgtcgtcg cgtgaggcgc cggccgttgc gccgactcca
    11521 tcggcgaagc caccggtcga gaagccctcg atcgtcgtgt tgccgtttca gaacctcagc
    11581 ggcaatccca cgcaggacta cttcggcgag ggcttcgccg aggacatcac gaccgctctg
    11641 tcacgtctga agtggttggt ggtcgcggcg cgagattcat cggacgcggc gtcgacgacc
    11701 gcggcagacc atcagcgcct cgcgcgggat cgcggcgtac ggtacgtgct caacggtagc
    11761 acgcggcttg ccggtgacca gctccgtgtc acctgtcagg tcatcgatgc ggagaattgg
    11821 cagaccatcc tgtcgcgcaa attcgaccgc cggctcggcg acgtgttcgc cgtgcaggac
    11881 gagatcaccg agagcctcgt tgccgtcatc gagccttcgc tgcttgacgc cgaagagatg
    11941 cgggcgcggc agcgaccgct ggtgcggctc gactgctggg gcctgatcgc gcgggccgtt
    12001 ggcctggtcc accggttcga gcgtgaggcg aacctcacgg cgcaagggca cctgcaacgg
    12061 gcgatcgcgc tcgatccgtc gagtgcgcgg gcgcaagcca cgctggcctg ggcgctctat
    12121 tgggcgtttc attgctcctg ggtggataac cgcgcaggat gcgctgagca ggccatggct
    12181 catgccgaga cggctcttcg gctcgatccc caggagccat gggcgagggc cgtgtatggt
    12241 ttcaatttga gttcgtctgg caagcatgcc gcggccatag acgaattgcg ccgggtgctc
    12301 gagctgcagc cgagcttcgc cctcggccgc atgttgctgg gctgggcgct gttgcggcga
    12361 ggcgatttca tagcggctat cggcgagacg caaagggcgt tgctgctcag tcccggcgat
    12421 cgcttcacgg ccgtctatca gcacgcacac ggcctcgccc tgttggccgc ccgccgcttc
    12481 gaggaagctt tgccgttcct gcgctcggcc gccgcgacct atgtcgaata catgggacac
    12541 gtcaacgggc tgatctcttg ctgcggccac ctcggcctcg tcgacgaggc gcggcacctg
    12601 atcgaatttc ggcgggcctg cctcggacgg gaattcacga ttgcttttgc gcgggccaga
    12661 ctttccgcgt tcgcgcactg cgacgtattc ctggaagggc tcgcgaaggc tggtgttcca
    12721 gactaatcgc tatttgcgcg accaaagaac gcaatgggca cgttgataga tagccctgcc
    12781 aagcgcttcg gcttcggccg cgcgaacacg agggtccttg tacaggctga tggaccacgc
    12841 gaacggcggt ctcgatcgca accgatgacg cgtggcgctg caagcatatt gcggcgcaca
    12901 atcagggccg acgctatgga ccgctcctgg cccgttgcga cgaacggagc tcgccgagcg
    12961 ataggtcctc gggacaagcc cgaggttgac agtcgtgggg ttggcaactt cagcgggcat
    13021 tgacaacctg ggtcggaagc acacccatac ggtcgtgcca ccgctacccc acccccatcg
    13081 acgaggaaag aagcggcgat cgccaggggc ggcgggcaga ggaactgccg aggatgcacc
    13141 gcgacaaggg cgtcgcgaga atttgacgcc gccgcgcgcc gaaactacgg tgcgcggctc
    13201 gtgcgcggcc gcgtggccgc tgcctggcac aagctggata cccgacgtca tgtccacctc
    13261 tgcaaccgcc gcgcttgccg ccgaggcccg cgcctggccc ttcgaggagg cccgccgcct
    13321 catccagcgg ctcgacaagt tgaagggtgg cagggacaag acggtcatct tcgagacggg
    13381 atacggtccc tccggcctgc cgcatatcgg caccttcggg gaagtggcgc ggacgacgat
    13441 gatccgccgg gcgttcgagg tgatcagcga gatccccacg cggctgctct gcttctcgga
    13501 cgacatggac ggcttccgca aggtgcccga taacgtcccg agcaaggaca tgctggcggc
    13561 acacctgcag aagccgctct cgcgcgtgcc ggatccgttc tgcaacgagc atgggagcta
    13621 cgcggcccac aacaatgcgg tgctgcgcgc cttcctcgac cgcttcggtt tccagtacga
    13681 gttcctgtcg gcgacggagg agtacacgtc cggccgcatg gacgaggcgt tgctccgcat
    13741 gcttgccggc tacgacaagg tgatggacat cattttgccg acgctggggc ccgagcggcg
    13801 cgcgacgtac tcgccgtttc tgcccgtctg cccgcgcacg ggnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcatatgtcg atccggaggg cggccagaag
    13921 gtcgagacca aggtcacggg cggggccgtc aagtgccagt ggaaggccga ctgggcgctg
    13981 cgctgggtcg cgctcggcgt cgactacgag atggcgggga aggacctgat cgacagcgtg
    14041 atgctgtcct cgaagatttg tcgcgcactc ggcggcacgc cgcccgaggg cttcaattac
    14101 gagctgttcc tcgacgagaa cggcgagaag atctcgaagt cgcgcggcaa tggcctcacg
    14161 gcggacgagt ggctgactta tgcgccgccc gagagccttt cgctcttcat gtaccagaag
    14221 ccgaagacgg caaagcgcct ctacttcgat cagatcccga aggcggtcga cgagtatctg
    14281 ggcttcgtct cggcctttcc gggtgagcca ggcgccaagc agctcgacaa tcccgcctgg
    14341 cacatccacg gcggccggcc gccgctgccc gaactgcctg tgagctttgc gctgatgatc
    14401 aacctcgtct cggcctcgaa tgccgcgaac aagtcgacgc tgtggggctt catcggccgc
    14461 tatgcgccgg gggcgagccc cgagaagaac ccgatcctcg accggctcgc gggctacgcg
    14521 atcgcctact accgcgattt cgtgttgccg acgaagacat accgcgcgcc gaccgcctct
    14581 gagaggaagg cactcggggc gctcgacgcc gagctcgcca aggcgagccc ggatgccagt
    14641 gccgaggcgc ttcagaccat cgtctacgag gtcggcaagg cgaacggcta tgccgagagc
    14701 ctgcgcgact ggttcaaggc gatctacgag gtgctgctgg gtcagacgca ggggccgcgc
    14761 ttcggctcgt tcatcgcgct ctacggcatc gccgagacgc gcgcgctgat cgccaagggg
    14821 ctcgcgggca agctcggcaa ggcatgaggg ctggcccatc ggggtgcggg cgttcatcgc
    14881 ctcttaatga ttcggccgtt agctcccgta ctgcgttctt gcatgggcac tcgaagtgtg
    14941 ccagggaacg ctagccggga gcgcaggggt tcgaattctt ttacggattt tcccccgctg
    15001 accatctgat taggcccttt gtggcacgtc cccactgcgc tcctgccgtt ggccatccgt
    15061 cgcacgcctg cggcgaagcc gcgcgttgtt cagccggaga aacttgcttt tggaacagac
    15121 gacgttcgct gggctcgggc tcagctccaa ggtgttggct gcgatcgacg cggtcggata
    15181 caccacgccg acgccaatcc aggcggaggc catccctgtc gccgtcaccg ggcgtgacgt
    15241 gctcgggatc gcgcagaccg gaacgggcaa gaccgcgtcc tacgtgctgc ccatgctctc
    15301 gcgcctcg
//
LOCUS       DAORNH010000077        15155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_2966_length_15155_cov_2.700662, whole genome shotgun sequence.
ACCESSION   DAORNH010000077 DAORNH010000000
VERSION     DAORNH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2966_length_15155_cov_2.700662"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..583)
                     /locus_tag="PK264_09850"
     CDS             complement(275..583)
                     /locus_tag="PK264_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76229.1"
                     /translation="MLRLLILLVAIAAGLVTALSVSNQLGGNAPGPAKTLLTTTATTF
                     PDWRSLAIGLVVGFALAWLRQFPWRLLPFLLSEWTRVWRRRAILACLAAGFVSVLLFY
                     "
     gene            762..1862
                     /locus_tag="PK264_09855"
     CDS             762..1862
                     /locus_tag="PK264_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRD76230.1"
                     /translation="MAKIAKVEPIVLKVPFTDGSSGTGLMPQAWTKLEIVLVRVETDD
                     GLVGWGESFGYFCSSAVAAAVRDMVAPVAIGQDAGDPRALNRMLQQRLHLHGRYGITI
                     FAISGLDIALWDIAGKRSGTSLTELLGGNARSASGGKVPAYASLMRYGDPVLVERFAA
                     KAASEGYADVKLHEIRRDTIGAGRKGVGAKVRLTTDINCNWSVDETRQILPYLKEIDL
                     FWLEEPIFPPEDWETLMELGKFGIAISAGENACTSVEFSRLIPAVTYAQPSVTKVGGV
                     SEVIAIADMAKRLGRTLMPHSPYFGPGYWATVHLVAARAELGLFEYLYVTPDAWLSPA
                     IPRPAKGVITPPTAPGLGFEPDQAVLERYRVA"
     gene            2065..2502
                     /locus_tag="PK264_09860"
     CDS             2065..2502
                     /locus_tag="PK264_09860"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HRD76231.1"
                     /translation="MRFVKAAAVGLALSLVASSAIAMHHKPKSLYDRLGGKPALRAVV
                     EDFVGNVAADARINGFFAKTNIPRLKRLLVEQLCAGTGGPCRYTGRSMKAAHKGMGVD
                     GAHFAALVEDLQKSLNKFKVPAKEQRELIAILAPMRKDIVEKR"
     gene            2682..3908
                     /gene="hemA"
                     /locus_tag="PK264_09865"
     CDS             2682..3908
                     /gene="hemA"
                     /locus_tag="PK264_09865"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HRD76232.1"
                     /translation="MDYHSTFASALDLLRTEGRYRVFADLKRAQGRFPLAERRAGDAR
                     QPVTVWCSNDYLGMGQHPKVLDAMHQALDEAGAGSGGTRNISGTTRYHVDLEAEIADL
                     HGKSAALLFTSAFVANEAALSTLQTLLPGVVVLSDEKNHASMIAGIRNGRGEKRIFRH
                     NDLADLERHLAAIEPGRPKIIAFESVYSMDGTISPIGGICDLARRYGALTYLDEVHAV
                     GLYGPRGAGIAERDGVMHRVDIINGTLAKGFGVMGGYIAASPEICDCIRSYAPGFIFT
                     TSVAPAIAAGAAASIRHLKSSSDERTQHQERVATVKRRLAAAGMPVLDNPSHIVPVMV
                     GDPKHCKMVSDTLLDRFAIYVQPINYPTVPKGTERLRITPTPCHSDADIDRLVRALST
                     LWAECELARGIRTDAA"
     gene            4054..4905
                     /locus_tag="PK264_09870"
     CDS             4054..4905
                     /locus_tag="PK264_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD76233.1"
                     /translation="MRCPSRATLASIAGAALSSVTLAGTSWSYDRPAGSSTTTAFGHP
                     PCYCDTARTPAYEDKRRPLVKAYPTTSPYGRTYVAPSYSVARDTRPVYVEPIAPVVTY
                     RRPPIWTGLYLGAHGGYTTGTAVLDGGRGSVGFDGMGGGIHLGYNWQSGGIVWGLETD
                     STWSNARGSSSMGGPTTMTVSHDWVSSLRARVGIPVDNAHLYLTAGAAAGHASWSAGG
                     LASSDTLFGLVLGGGLEYRLHEALSLRIEGLHYMFGDRTVSLPGGNARVDADMTTFRA
                     GLSLHFN"
     gene            5028..7001
                     /locus_tag="PK264_09875"
     CDS             5028..7001
                     /locus_tag="PK264_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRD76234.1"
                     /translation="MTSNNFQERAAGLVASLVALPRWTKRGLLAASDALLLGLALWIS
                     FVFRFNTLDWPAVYWPDSPAFIALLLAAPVMGVATFHLMSLYRLVTRFFGAKGSARIA
                     MAMLFAVTAWAALGAMLGPTGMPRTVILLYAILGTLFVILSRQVAAYVLKIETPVARG
                     PGRPVLIYGAAGPGLALLDALNRSGTYDPVGLLDDQPSLWGQRIAGCKVYRPDKISRL
                     IERHGVRDVLLALPAGRRRERQAVIRALGAYPVRVKTLPAAEDVASGRVAVSDLRPVD
                     VEDLLGRDPVPADAELLTRDIERKSVLVTGAGGSIGSEITRQVLRLAPSRLVLLDASE
                     AALYEIEMEVRRLNEGGRVTVAAVLGSVLDKATLDQTIAAHRVSTIYHAAAYKHVPIV
                     EQNPFIGIANNTFGSLVVAEAAARGGVGKVVLVSTDKAVRPTNIMGASKRLAELIFQA
                     FAARNRETVFTMVRFGNVLDSSGSVVRLFRKQIEGGGPVTVTDPKMIRYFMSIPEAAT
                     LVIQAGAMAKGGEVFVLDMGEPVKIDELARSMIRLMGLEVREPGSQDGDIAISYIGAR
                     PGEKLYEELLIGENTTTTHHPRIMRCDEPSLTARELEAVLEELRQAMRSGDLAEIRAL
                     LMRTVDYQPVVTAEARKAPPDLMTLEPPSKAIN"
     gene            7010..8158
                     /locus_tag="PK264_09880"
     CDS             7010..8158
                     /locus_tag="PK264_09880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD76235.1"
                     /translation="MIPDSGARRVAIVTDDAGRLARIASPLIEAILGRGHHVRVFAQR
                     SADGSTAAWLAGRGIDTLPIGVDPETSNPLTDRAVLKLLTKALADYEAHALLALGDRA
                     HLQAARAASRARIARAVLLVDTLADLELKHGDKPGWLSRYSLRTSLAGASSILFSDNQ
                     DRRDADLAGIIPPAVATAELPGLGIDLAANPVLPLPPTAPGLTFAMLAALDRRNGIGA
                     YLEAARIVRGEMPSASFVLAGAEGRGPSAFPLRHLEAFQAAVRYIPSPFDFADVLGAA
                     HVVVAPLTYGGAPYELLQGLAAGRPIITTATPGARLAVDERINGCLVPPSDPEALAQA
                     IRSFFAHPELLPFEARASRLKAERRFDVRGVIPQVLAALHLGTASGAT"
     gene            8158..10110
                     /gene="asnB"
                     /locus_tag="PK264_09885"
     CDS             8158..10110
                     /gene="asnB"
                     /locus_tag="PK264_09885"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRD76236.1"
                     /translation="MCGIAGLVDPSLSPGEIARLAERMAQSILHRGPDEGQTWVEPDA
                     GVALAFRRLAIVDLSTAGRQPMTSDSRRYVIVYNGEIYNAEEMRRALGADVPCWRGHS
                     DTEVLLEWIARRGIASALEKANGMFALALWDRSERRLTLARDRMGKKPLYWWRDGRRI
                     VFGSELRALGCHPALPRGIDRSALAGYLRHGYMLHPYTIYAGVGQLPPGHVVTIGPGG
                     GGEPQPFWSLAEVARRGRRELFAGDDAAALEELGNRLGSAVRRRLVSDVPLGAFLSGG
                     IDSSTIVALMQQGAAAPVRTFAIGFEEDAFNEAPHAAAVARHLGTDHTELVVTAKAAL
                     DVIPRLADIYDEPFADSSAIPTCIVSALARQHVTVALSGDGGDELFAGYDRYRDAERL
                     MRTAGRLPLPLRRLGSSAVSRLSTASLDLMIRPLPERVRRRLSGDRLKKLGAIAGHSG
                     DELYRALISIWPDPAAVLNPDLGPVAEPASALTDTALPDLIAEPISRLAYRDARSYLP
                     DDILTKVDRASMAVSLEARAPLLDPEVVAFAYALPERFKVRDGEAKWLLRRLAYKLVP
                     RPLLDRPKSGFAVPIGTWLRGPLKDWADDLVSERSLRDSGLLDPAVVRTTWSEHLAGA
                     RNWSFQIWTVLMLLAWQRRHGGTHAG"
     gene            10100..>10633
                     /locus_tag="PK264_09890"
     CDS             10100..>10633
                     /locus_tag="PK264_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76237.1"
                     /translation="MRARAVAVLAAVLIAAGLPQTSQAERPGLTHVPAGALAVFHRAL
                     DDLKMGKRDLVTIIQIGDSHTANDHFTGRLRTRLQAIYGDAGRGLMQPGEPFAYFRPY
                     QVRVVQKGPWEQFTSNRAAPDVAPFGLAGMILRGRTPQSTIEIETTDGEAIGRLAVDF
                     YLQPGGGSFEVRAGGARL"
     assembly_gap    10634..10652
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10653..11382
                     /locus_tag="PK264_09895"
     CDS             <10653..11382
                     /locus_tag="PK264_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76238.1"
                     /translation="DYRIALTSIPLPAMASRVTLAPLGDGPVDLAATGLEPERRGVRV
                     LSFGFPGAQASILDRWQFDNIAFQLRRLAPSLLIVAFGTNEGFQVAERLEGYGPAFDR
                     RTALLRRGAPDASLVIVGSPAAHRLPRHCALTPEAQDLAPCSPLSASERARYDLMMAR
                     ADPALCRWHTPAALTTIRADQAAAAKRLGATFWDWSALMPSECGLDDWYRRSLAHRDR
                     VHLRQDGYWRSADDLLAHLLNGAR"
     gene            11519..12193
                     /locus_tag="PK264_09900"
     CDS             11519..12193
                     /locus_tag="PK264_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HRD76239.1"
                     /translation="MPQSTNVSRRAALTGLVMAGSAAVVTHALAQRSGELVVTPSQTE
                     GPYYPVDWSGDIDHDLVEVKGQPSKAAGPVAHVSGRVLDLAGQPIAGARVEIWQCDNK
                     GIYRHPADRGAGRAPDPGFQGHGRVETGSDGRYAFRTLRPAIYPGRAPHIHFKVRVPG
                     SPTLTTQLYVHGDPANERDGVLSRIRDRRQRESVIARFEPADAVEAGALAATFDIVIA
                     RAPRRA"
     gene            complement(12210..13265)
                     /locus_tag="PK264_09905"
     CDS             complement(12210..13265)
                     /locus_tag="PK264_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD76240.1"
                     /translation="MAHARRIALNLALQGGGVHGAFTWGVLDRLLDDEAIEFRWVSAG
                     AMNATALASGLLAGGRQAARERLSALWRGVSAFASRDVTRLNPLLFGLTRAAMGTMNG
                     IVSPYEFNPLGVDPLRELVERVIDFKALAGAHAKLDLVITATDVATGRARRFRRAEIS
                     SDVLLASACLPTLHHAVEIGGRAYWDGGFSANPDLLEIAGASRVGDTLMVVLNPLDAP
                     GTPRGAREIAGAANRITFNQPLLRDVDAILAARRQGRGWFHRRTREHAIAAHRFHMIT
                     AGRYTAALSHDSKSKPDAELIGYLYNVGRGETEKWLANARQHVGLKDTADLETRFALP
                     DFAPARRPLATKAEAAE"
     gene            complement(13406..13648)
                     /locus_tag="PK264_09910"
     CDS             complement(13406..13648)
                     /locus_tag="PK264_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76241.1"
                     /translation="MLMRSVSLAAAGAALATLLVSIDAAEAACPASAGRASLDIWPRD
                     TLKSGQTVSATHPCGKRLTCTSHGRSGIGARKCSWG"
     gene            complement(13801..14031)
                     /locus_tag="PK264_09915"
     CDS             complement(13801..14031)
                     /locus_tag="PK264_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76242.1"
                     /translation="MRRALVIAAFAAVPVLIPQLAEAACPANPRAASLAIWPRGTIAA
                     GRTVTARHPCGRYLMCTAGTLDQSGSRNCQWL"
     gene            14225..>15155
                     /locus_tag="PK264_09920"
     CDS             14225..>15155
                     /locus_tag="PK264_09920"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRD76243.1"
                     /translation="MQKIKVAGTVVEMDGDEMTRIIWKAIKDKLIHPYLDVKLEYYDL
                     SVEMRDKTNDQITIDSANATKKHGVAVKCATITPDEGRVKEFNLKEMWRSPNGTIRNI
                     LGGVIFREPIICKNVPRLVPGWTKPIIIGRHAYGDQYRATDFKFPTKGTLSIKFVGDD
                     GKVIEREVFKAPGGGVTMAMYNLDDSIREFARASMNYALGRGYPLYLSTKNTILKQYD
                     GRFMELFQEVFDKEFKAKYEAAKITYEHRLIDDMVAAALKWSGGYVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTV"
BASE COUNT         2440 a         5195 c         5003 g         2498 t
ORIGIN      
        1 gatcggcccc tctcacggac atcacatgcc gaaccaactg ggacgcccgc cattgccgcg
       61 ggcgttcctc gttccgggtg gcggattgca accgggctta gggtcgacag gcggctccga
      121 gtagcacgaa gcttccagac gcaggactgt tgatcgcaag gctcgggttg cgacgcgggg
      181 gcacgcgtct caggttctca gccgggcctc gcgtagcggc gcgcctgctc gggcgcgcgc
      241 gagcttcagg gcgtgcctcg acgagggttc gatctcagta gaacagcagg accgacacaa
      301 agcccgcggc gaggcacgcc aggatggcgc gtcggcgcca gactcgcgtc cattccgaga
      361 ggaggaaggg cagcaggcgc cacgggaact gccgcaacca ggcaagcgcg aacccgacga
      421 cgaggccgat cgccagcgag cgccagtcgg gaaacgtggt cgcggtcgtg gtcagcaacg
      481 tcttcgccgg gccgggcgcg tttccgccca gctggttgct gacggacagt gccgtgacca
      541 gcccagcagc gattgccacc agcaggatca gcaatctcag catgcggacc cccaccacat
      601 gaaagctctc ggcacgtgcc tcggtaaaat cgagtgggtg ggagagcgga cggcattgcg
      661 cttcgggtta gcatgtcgtg gtgaaaatac ggtcaacagg cgcctcgtct ccggtcgcat
      721 tgcgatctaa ggtggagtgg gaaagagctg cggagagcgc catggcaaag attgccaaag
      781 tcgagccgat cgtcctcaag gtccccttca cggatggcag ctccgggacg gggctcatgc
      841 cgcaggcctg gaccaagctc gagatcgttc tcgtgcgggt ggaaacggat gacggcctcg
      901 tcggctgggg tgagagcttc ggctatttct gctcgagcgc cgtggcggcg gcggttcgcg
      961 acatggttgc tccggtggcg atcggccagg acgccggcga tcccagggcc ctcaatcgca
     1021 tgctgcagca gcgcctgcac ctgcatgggc gctacggcat tacgatcttc gcgatctctg
     1081 gcctcgacat cgcgctctgg gatatcgcgg gcaagcgatc ggggacgagc ctcaccgagc
     1141 ttctcggcgg caacgcccgc tcggcgagcg gcggcaaggt gccggcctat gcgagcctga
     1201 tgcgctacgg tgatccggtg ctcgtcgagc gatttgcggc gaaggcggcc agcgagggct
     1261 atgccgacgt caagctccac gagatccggc gcgacacgat cggggctggc cgcaaggggg
     1321 tcggagcgaa ggtgcgcctc acgaccgaca tcaactgcaa ctggtcggtg gacgagacgc
     1381 gccagatcct cccctatctc aaggagatcg acctcttctg gctcgaggag ccgatttttc
     1441 cgccggagga ctgggagacg ctgatggagc tcggcaagtt cggcatcgcc atctcagctg
     1501 gcgagaatgc ctgtacgagt gtcgaattct cgcgcctgat acctgccgtc acctacgcgc
     1561 agccgagcgt caccaaggtc ggcggcgtga gcgaggtgat cgccatcgca gacatggcaa
     1621 agcggctcgg gcgcacgctc atgccgcact ctccgtattt cgggccaggc tactgggcga
     1681 ccgtgcacct cgtcgcggcg cgcgccgaac tcggcctctt cgagtacctc tacgtcacgc
     1741 ccgatgcctg gctgtcgccg gcgatcccgc gtcccgcaaa gggcgtcatc acgccgccca
     1801 ctgcgccggg cctcggcttt gagccggacc aggcggtcct cgagcgctac cgggtcgcct
     1861 gagccctcat cacgacgacg tgaactccgg ccgccatcca tcgccgaccg ggcgggcgta
     1921 atcacttgcg accaccgaac gacgatggtc gagcccgaca gcaacgagcc ctggtccccg
     1981 acggggtccg cttcggcgcc agcgcgccga gggatcggct ttgcgcgccg gctcaactcc
     2041 aggcacctac tcgggagagt tctgatgcgc tttgtgaaag ccgctgccgt tggccttgcc
     2101 ctctcgctcg tcgcctcgag cgcgatcgcc atgcaccaca agccgaaatc gctctacgac
     2161 cgcctcggtg gcaaaccggc actgcgcgcc gtcgtcgagg atttcgtcgg caatgtcgcg
     2221 gccgatgccc gcatcaacgg tttcttcgcc aagacgaaca ttccgcgcct gaagcgcctg
     2281 ctcgtcgagc agctctgcgc gggcaccggc ggcccgtgcc gttatacggg ccgcagcatg
     2341 aaggccgcgc acaagggcat gggggtcgac ggtgcgcact tcgccgcact tgtcgaggac
     2401 ctccagaaat cgttgaataa attcaaggtt ccggcgaaag agcagcgcga gctgatcgcc
     2461 atcctcgccc caatgcggaa agacatcgtc gagaagcgat gatacgactt gatccagatc
     2521 acaatccgac cgcgccgccc cactagcttg ggcggcgggc gctggcgcgg cggacaccgt
     2581 acacccgatc acgcggcgaa cgaaaattcg cagatgtgcc aaggggggcg acggcccgtc
     2641 ggcccggagc tatatccgac ttgagcagtg gaaccagagc gatggactat cacagcacgt
     2701 ttgcgtcggc gctggatctg ctccgaaccg agggccggta tcgagtgttc gccgacctta
     2761 agcgggcgca gggccgcttc ccgctcgcgg agcgccgcgc tggcgatgcc cgccagccgg
     2821 tgacggtctg gtgctcgaac gactatctcg gcatgggcca gcaccccaag gtcctcgatg
     2881 ccatgcacca ggcgcttgac gaggccggcg cgggctcggg cggcacgcgc aacatctcgg
     2941 gcacgacccg ttatcacgtc gacctcgagg ccgagatcgc cgacctccat gggaaatcgg
     3001 cagcccttct gttcacgtcc gccttcgttg ccaacgaggc ggcgctctcg acgctgcaga
     3061 cgctgctccc cggcgtggtc gtcctttcgg acgagaagaa ccacgcctcg atgatcgccg
     3121 gcatccgcaa cggtcgcggc gagaagcgca tcttcaggca caacgacctc gccgatctcg
     3181 agcggcatct cgcggctatc gagccgggcc ggccgaagat catcgctttc gagtccgtct
     3241 actccatgga cggcacgatc tccccgatcg gcggcatctg tgatctcgca cgccgctacg
     3301 gggcgctcac ctacctcgac gaggtgcatg cggtcggcct ctatggaccg cgcggcgccg
     3361 gcattgccga gcgcgacggt gtcatgcacc gggtcgacat catcaacggt acgctcgcca
     3421 agggctttgg cgtgatgggc ggctacattg ccgcatcccc ggaaatctgc gactgcatac
     3481 gctcctacgc gccgggcttc atcttcacga cttcggttgc tcctgcgatc gccgcaggcg
     3541 ctgccgcctc gatccgccat ttgaagtcgt catcggacga gcggacgcag catcaggagc
     3601 gtgttgcaac cgtgaagcga aggcttgccg cggcaggcat gcctgtcctc gacaatccga
     3661 gccatatcgt gccggtcatg gtcggcgacc cgaagcactg caagatggtc tccgacacgc
     3721 ttctcgaccg gttcgccatc tatgtgcagc cgatcaacta cccgaccgtg ccaaagggca
     3781 cggagcggct ccgcatcacg ccgacgccgt gccacagtga cgctgatatc gatcgcctcg
     3841 tgcgggcgct gtcgacgctc tgggcggagt gcgagctcgc acgcggcatc cgcacggacg
     3901 ctgcctgatc ctgatcgcgg atcaggggcc ggcttgcaag gtcagccggc tgacttaacg
     3961 atttgctaac cattctttac gacaacctat gcgtgcggtt gaccggacca gtggccgcaa
     4021 tccgagcgtg gggttgagcg taaggaatag cgtatgcgtt gtcccagccg ggccaccctc
     4081 gcctcgatcg cgggcgcggc tctgtccagt gtcactcttg ccggcaccag ctggtcatat
     4141 gaccggccgg ccggctcgag cacaacaacc gccttcggcc atccgccttg ctactgcgac
     4201 acggcccgca ccccggcata cgaggacaag aggcgcccgc tcgtcaaggc ctacccgacc
     4261 acttcgccct acgggcgaac ttatgttgcg ccgagctaca gtgtcgcccg ggacactcga
     4321 cccgtctatg tcgagcccat cgcgccagtg gtgacataca ggcgccctcc catctggaca
     4381 ggcctgtacc tcggtgcgca cggcggctat acgacaggca cagcggttct cgacggcggg
     4441 cggggcagcg tcggcttcga cggcatgggc ggcggcatcc acctcggcta caactggcaa
     4501 tcgggcggca tcgtctgggg tctcgagacc gacagcacct ggtcgaatgc acgtggaagt
     4561 agcagcatgg gcggtccgac cacgatgacc gtctcgcacg actgggtgtc ctcgctccgc
     4621 gcccgcgtgg gcatccccgt cgacaacgcc cacctctatc tgacagcggg cgctgccgcc
     4681 ggccatgcca gctggtcagc cgggggcctc gcctcctccg ataccctctt cggcctcgtc
     4741 ctcggcggcg ggctcgaata tcgcctgcac gaagccctaa gtctgaggat cgagggcctc
     4801 cactacatgt tcggcgatcg aacagtttcg ctgccaggcg gaaatgcccg cgtcgatgcg
     4861 gacatgacga cgttccgcgc cggcctctcg ctgcatttca attgaacgtc cggtctccct
     4921 atagccggat gtcgcgccta tccccagtgc tgttgccgca ttttggcacc tgatccttcg
     4981 gatgctatga agcgggaagg tgcaaggcga tgggtccggc ggccggcatg acgtcgaaca
     5041 attttcagga aagggctgcg gggctcgtcg cgagcctcgt cgccttgccg aggtggacga
     5101 agcgcgggct gctcgcggcc agcgacgcgc tcctgctcgg cctggcgctc tggatatctt
     5161 tcgtgtttcg tttcaatacg ttagattggc cggccgtcta ttggccggac agccccgcct
     5221 tcatcgcgct cttgctcgcc gcgcctgtga tgggcgttgc gacgttccat ctcatgagcc
     5281 tctaccggct cgtcacccgc ttcttcggcg ccaagggctc ggcgcgaatc gccatggcga
     5341 tgctctttgc cgtgacggca tgggcagccc tcggcgccat gctcggcccg accggcatgc
     5401 cgcggaccgt catactcctc tacgcgatcc tcggcaccct cttcgtgatc ctgagccgac
     5461 aggtcgcggc ctatgtcctg aagattgaga cgccggtcgc gcgcggacct ggacgcccgg
     5521 tcctgatcta cggtgccgcc ggacccgggc tcgcgcttct cgatgccctc aaccggtcgg
     5581 gcacatatga tccggtcggt ctgctagacg atcagccctc gctctgggga cagcggattg
     5641 ccggctgcaa ggtctatcgt cccgacaaga tttcgcggct gatcgagcgg cacggcgtgc
     5701 gtgacgtgct gctggcgctg cccgccggcc gacgccgtga gcggcaggcc gtcatccggg
     5761 cacttggcgc ctatcctgtt cgcgtgaaga cgctgccggc cgcggaggac gtcgcgagtg
     5821 ggcgcgtcgc ggtgagcgat ctccgcccgg tcgatgtcga ggatctgctc ggtcgtgatc
     5881 cggtgccggc ggacgctgag ctgctgaccc gcgacatcga gcggaagtcg gtgctggtaa
     5941 ccggcgccgg agggtcgatc ggctccgaaa tcacgcggca ggtgctgcgc ctcgcgccgt
     6001 cgcgactcgt gttgctcgat gcctcggagg cggccctcta cgagatcgag atggaggtgc
     6061 gccgtctcaa cgagggaggc cgcgtgacgg ttgccgccgt cctcggctca gtgctcgata
     6121 aggcaacgct cgatcagacg attgccgcgc atcgcgtctc gaccatctat catgccgccg
     6181 cctacaagca tgtgccgatc gtcgagcaga acccgttcat cggcattgcc aacaacacgt
     6241 tcggctcgct ggtggtcgcc gaggcggcag cgcgcggtgg ggtcggaaag gtcgtgctgg
     6301 tctcgaccga caaggccgtc aggccgacga acatcatggg cgcgagcaag cgcctcgccg
     6361 agctcatctt ccaggccttc gcggcgcgga accgcgagac cgtcttcacc atggtgcgct
     6421 ttggcaatgt gctcgacagc tcgggctccg tagtgcggct ttttcgcaag cagatcgagg
     6481 ggggcggtcc ggtcacggtc accgatccga agatgatccg ctacttcatg tcgattcccg
     6541 aggccgccac cctggtcatc caggccggcg ccatggccaa ggggggcgag gtgttcgtgc
     6601 tcgacatggg cgagcccgtg aagatcgacg aactcgcccg ctccatgatt cgcctgatgg
     6661 gcctcgaggt gcgcgagcct ggcagccagg acggcgacat cgccatctcc tacatcggcg
     6721 cgcggccggg cgagaagctc tacgaggagc tcttgatcgg cgagaacacc acgacgacgc
     6781 accacccgcg cattatgcgc tgcgacgagc cctcgctcac ggcccgcgag ctcgaggcag
     6841 tgcttgaaga gctccgtcag gccatgcgct cgggcgacct cgccgagatt cgcgccctcc
     6901 tgatgcggac ggtcgactat cagccggtcg tcacggccga ggcccgcaag gcaccaccgg
     6961 acctgatgac gctcgagccg ccgtcgaagg ccatcaactg atgccacccg tgatcccgga
     7021 ctccggcgca cgtcgtgttg ccatcgtcac ggacgacgcg ggccggctgg cgcgcatcgc
     7081 ctctccactc atcgaggcga tcctcggacg cgggcatcac gttcgcgtct ttgcccaacg
     7141 cagcgcggac ggcagcacgg cagcgtggct ggccgggcgc ggcattgata cgctgccaat
     7201 cggcgtcgac ccggagacga gcaatccgct gaccgaccgg gcggtgctga agctgctcac
     7261 gaaggcactt gccgactacg aggcccatgc gctcctggct ctcggcgacc gcgcccactt
     7321 gcaggctgcc cgggccgcga gtcgcgcccg cattgcgcgt gccgtgctgc tggtcgacac
     7381 gctcgccgat ctcgagctga agcacggcga caagcccggc tggctctcgc gctactcgtt
     7441 gcgcacatcg ctcgcgggag cgtcgagcat tctgttctcg gacaaccaag atcgtcgcga
     7501 cgccgacctc gcgggcatca tcccgccggc cgtggcgacc gccgagctgc ccggtctcgg
     7561 catcgatctc gcggcgaacc cggtgctgcc gctgccgccg actgcgccgg gcctcacttt
     7621 cgcaatgctc gccgcactcg accgtcggaa cggcatcggc gcatatctcg aggcggcgcg
     7681 gatcgtgcgg ggcgagatgc cgtccgcctc attcgtgctc gccggtgccg aggggcgcgg
     7741 gccaagtgcc tttccgctcc gccatctcga ggcgttccag gcggcggtcc gctacattcc
     7801 ttcccccttc gactttgccg acgtgctcgg cgccgcgcat gtcgtcgtcg cgccgctcac
     7861 ctacggcggc gcgccgtacg aactgctcca ggggcttgcg gcgggccggc ccatcatcac
     7921 cacggcaacg cccggcgcgc gtcttgccgt cgacgagcgg atcaacggct gcctcgtccc
     7981 gccgagcgac ccggaggcgc tcgctcaggc gatccgctcc ttcttcgccc atcccgagct
     8041 tctgccgttc gaggcacgcg cgagccgcct caaggccgag cggcgcttcg atgtccgcgg
     8101 cgtgatcccg caggtcctcg ccgcgctcca cctcggcaca gcgagcggag ccacctgatg
     8161 tgcggcatcg ccggtctcgt cgatccgagc ctttctcccg gcgagattgc gcgcctcgcc
     8221 gagcggatgg ctcagagcat ccttcatcgc gggcccgacg aagggcagac ttgggtcgag
     8281 ccggacgccg gcgtcgcgct ggccttccgg cgcctcgcga tcgtcgatct cagcacggcc
     8341 gggcggcagc cgatgacgtc ggactcgagg cgctacgtta tcgtctacaa tggcgagatc
     8401 tacaatgccg aggagatgcg ccgcgcactc ggcgcggacg tgccctgctg gcgcggccat
     8461 tcggacacgg aggtgctgct cgagtggatc gcgcggcgcg gcatcgcctc cgcgcttgag
     8521 aaggcgaacg gcatgttcgc gctggcgctc tgggaccggt cggagcgccg cctgacgctc
     8581 gctcgcgatc gcatggggaa gaagccgctc tactggtggc gcgatggccg gcgcatcgtc
     8641 ttcggctcgg agttgcgtgc gctcggatgc catccggcct tgccgcgcgg gatcgatcga
     8701 tcggcactcg ccggctacct ccgccatggc tacatgctgc acccatacac gatttacgcc
     8761 ggcgtcgggc agctgccacc cgggcacgtc gtgaccatcg gaccgggcgg aggcggcgaa
     8821 ccgcagccgt tctggtcgct tgccgaagtt gcgcggcgcg gccgccgcga gctatttgcg
     8881 ggtgacgatg ccgcggcctt ggaggagctc ggcaaccgcc tcggcagtgc cgtgcgccgc
     8941 cgcctcgtct ccgacgtgcc gctcggcgcg tttctctcag gcggcatcga cagctcgacc
     9001 atcgtcgcgc tgatgcagca aggggccgcc gcgcctgtgc gcacgtttgc gatcggtttc
     9061 gaggaggatg ccttcaacga ggcgccgcac gcggccgccg ttgcccgtca ccttggcacc
     9121 gaccacaccg agctcgtcgt cacggcgaag gccgcgctcg acgtcatccc gaggctcgcc
     9181 gacatctatg acgagccgtt cgccgacagc tcggcaatac cgacctgcat cgtctcggcg
     9241 ttggcgcggc agcatgtgac ggtggcgctc tcgggtgacg ggggcgacga gctgttcgcc
     9301 ggctatgatc gctatcgcga cgcagagcgg ctgatgcgga cggctggtcg cctgccgctg
     9361 ccactccggc gcctcggatc gagcgcagtg tcgcggctct cgacggcgag cctcgacctg
     9421 atgatccgac cgctaccgga gcgcgttcga cgccgtttga gcggcgatcg cctgaagaag
     9481 ctcggggcaa tcgccgggca ctcgggcgac gagctctacc gcgcgctgat cagcatctgg
     9541 cccgacccgg ctgccgtcct gaacccggac ctcgggcctg tcgccgagcc tgcttccgcg
     9601 ctcacggata cggcgctccc cgacctcatc gccgagccga tctcgcgtct cgcctatcgt
     9661 gacgcgcgct cctatctgcc ggacgacatc ctgacgaagg tcgaccgcgc cagcatggcg
     9721 gtctcgctcg aagcgcgtgc gccgctcctc gatcccgagg tggtcgcgtt cgcctatgcg
     9781 ctgcccgagc gcttcaaggt tcgcgacggc gaggcgaagt ggctgctgcg tcggctcgcc
     9841 tacaagctcg tcccgcggcc gctgctcgat cggccgaaga gcggctttgc cgtgccaatc
     9901 ggcacttggc tcagaggacc gctcaaggac tgggcggacg atctcgtttc ggagcggtcg
     9961 ctccgcgaca gcggcctcct cgatcctgcc gtcgtgcgta caacgtggtc tgagcacctc
    10021 gccggcgcgc gcaattggtc gttccagatc tggacggttc tcatgctgct cgcctggcag
    10081 cgtcgccatg ggggaacgca tgcgggctag ggccgttgcc gtcctggcgg ccgtcctgat
    10141 cgccgcaggc ctgcctcaga caagccaggc ggagcggccc ggtctcacac atgtccccgc
    10201 cggggcgctg gcggtcttcc atcgggcgct cgacgacttg aaaatgggca agcgtgatct
    10261 cgtcaccatc atccagatcg gcgacagcca caccgcgaac gatcacttca ccgggcggct
    10321 gcgcacgcgc ctccaggcga tctacggtga cgccgggcgc ggcctgatgc agccgggcga
    10381 gccctttgcc tacttccggc cctatcaggt gcgcgtcgtc cagaaggggc catgggagca
    10441 gtttacgtcc aaccgggcgg cgccggacgt tgcgccattc ggccttgccg gcatgatcct
    10501 gcgcggccgc acaccgcaat cgacgatcga gatcgagacg acggacggcg aggccatcgg
    10561 ccggctcgcg gtggacttct acctgcagcc cggcggcggc agctttgaag tgcgtgcggg
    10621 cggcgcacgg cttnnnnnnn nnnnnnnnnn nnggactatc gcatcgcgct gacgtccatt
    10681 cccctgccgg cgatggcctc gcgcgtgacg cttgccccgc ttggtgacgg cccggtcgat
    10741 ctcgccgcga cagggcttga gcccgagcgc cgcggggtac gtgtcctgag tttcggcttt
    10801 cccggggcgc aagcctcgat cctcgaccgc tggcagttcg acaacatcgc ctttcagctc
    10861 cgccgcctcg cgccgtcgct gctcatcgtc gcgttcggca ccaacgaggg cttccaggtc
    10921 gccgagcggc tcgagggcta cggtccagct ttcgatcgac gcaccgcgct gctcaggcgc
    10981 ggtgcgccgg atgcctcgct cgtgatcgtt ggctcgccgg ccgcgcaccg cctgccgcga
    11041 cactgcgcgc tgacacctga agcccaggat ctcgcgccgt gctcgcctct ctcggcgagc
    11101 gaacgcgcac gctacgatct catgatggcg cgtgccgatc ccgcgctctg ccgctggcat
    11161 acgcccgcag cgctgacgac cattcgcgcg gatcaggcgg cggcggccaa gaggctcggc
    11221 gccacgttct gggactggtc ggcgttgatg ccgtcggagt gcggactcga cgactggtac
    11281 cgccggagcc ttgcgcatcg cgatcgcgtc catctccgcc aggacggcta ctggcggagc
    11341 gcggacgatc ttctggcgca tctcctcaat ggcgcgcgct gaggtcgccg gtcagcggcg
    11401 cggcgcccgg atcacggcga attgatgcgc ggggcatgcc gcctccccgc tgccgtcacc
    11461 atctcagcct ccgagtaggc ataggaggcg ctcgcctcaa ccggaaacgg agattcccat
    11521 gccgcagtca acaaatgtca gccgtcgcgc ggcgctgacc ggtctcgtga tggccggatc
    11581 ggcagctgtc gtgacgcatg cactcgccca gcggagcggc gagctcgtcg tcacgccgtc
    11641 ccagaccgaa ggcccctact atcccgtcga ctggtccggc gacatcgacc atgacctcgt
    11701 cgaggtgaag ggccagccgt caaaggccgc cggacccgtt gcgcatgtca gcggtcgcgt
    11761 cctcgacctc gccggccagc cgatcgccgg cgcgcgcgtc gagatctggc agtgcgacaa
    11821 caagggcatc taccgccacc cggccgaccg cggcgccggc cgcgcgcccg accccggctt
    11881 tcaggggcac ggtcgcgtcg agaccggcag cgatggacgt tatgccttcc gcacgctgcg
    11941 ccccgcgatc tatccgggac gtgcgccgca catccacttc aaggttcgcg ttccaggtag
    12001 cccgacgctc acgacccaac tctacgtgca cggcgatccg gccaacgagc gggatggcgt
    12061 gctgagcagg atccgcgatc gccgacagcg cgagagcgtg atcgctcgct tcgagccggc
    12121 cgacgccgtc gaggccggcg cgcttgccgc gaccttcgac atcgtcatcg cgcgggcacc
    12181 tcggcgggcc tgacgcgtcc cggagacact cactcggccg cttccgcctt ggtcgcgagc
    12241 ggacgccgcg ccggcgcaaa atcggggaga gcgaagcgcg tctcgagatc ggcagtatcc
    12301 ttcagtccga catgctggcg cgcgttcgcc agccacttct cggtctcgcc gcgaccgaca
    12361 ttgtaaaggt agccgatgag ctcggcgtcg ggcttcgact tgctgtcgtg gctcagtgcc
    12421 gccgtgtagc ggccagccgt gatcatgtga aagcggtgcg cggcgatcgc gtgctcgcgc
    12481 gtgcgcctgt gaaaccagcc gcgcccctgc cgacgcgcag cgaggatggc gtcgacgtcg
    12541 cgcagcagcg gctggttgaa tgtgatgcgg ttggcggcgc cggcgatttc acgtgcgccc
    12601 cgtggcgtgc ccggcgcatc gagcgggttc aggaccacca tcagcgtgtc gccgacccgg
    12661 ctcgcgccgg cgatctcgag gagatccgga ttggcggaaa acccgccgtc ccaataggcc
    12721 cggccgccga tctccacggc atgatgcagg gtcggcaggc acgccgaggc gagcaggacg
    12781 tcggaggaaa tctcggcacg gcggaaacgg cgcgcgcgcc cggtcgcgac gtccgtcgcc
    12841 gtgatgacga ggtcgagctt tgcgtgcgct ccggcgagtg ccttgaagtc gatgacacgc
    12901 tcgacgagct cgcgcagcgg gtcgacaccg agaggattga actcgtaggg cgagacgata
    12961 ccgttcatcg tgcccattgc ggctcgggtg agcccgaaga gcagcggatt gaggcgcgtc
    13021 acgtcgcggc tcgcaaaggc gctgacaccg cgccagagcg ccgacaggcg ctcgcgcgcg
    13081 gcctgccgcc caccggcgag aagtcctgaa gcgagcgctg ttgcattcat ggcgccggcg
    13141 ctcacccagc ggaactcgat cgcctcgtca tcgagaaggc ggtcgagcac gccccaggtg
    13201 aaggcgccgt gcacgccgcc tccctgcaaa gccagattga gcgcgatccg cctcgcgtgt
    13261 gccacctgct gccccctctg ctgccggctc tgtggcacgt gcccgcggca ctgtcgagac
    13321 cgacaatcga aggagcgcta tcagcccgct ctgtgacgcg agcgaggcac gcgcgcgggc
    13381 agatggtcgc ggtggccgag aggcatcagc cccagctgca tttgcgagca ccgatgccgc
    13441 tgcgcccatg actcgtgcag gtgagccgct ttccacaggg gtgcgtcgcc gagacggtct
    13501 gaccggattt cagcgtgtcg cgcggccaga tgtcgaggct cgcgcggcct gcactggcag
    13561 ggcaggcggc ctcggcggca tcgatcgaga cgagcagcgt ggcgagcgcg gcaccggccg
    13621 cggcaaggga aacagagcgc atgagcatgg caaaacctca tcgacaaagt agctgcaacg
    13681 gtcattgcat caatgacgat cctacgcgcg ccggaggtcg tagacaagtc tgcctgcaga
    13741 ctattgccgt gggcgaggcc ccatctgaca cgacacgaca gccggtcata gctcgccgtc
    13801 tcaaagccat tggcagttgc ggctgccgct ctggtcgaga gttccggccg tgcacatgag
    13861 atagcgcccg caagggtgac gcgctgtcac cgtgcgcccg gcggctatgg tgccgcgtgg
    13921 ccagatcgcg aggctcgcgg cccgcgggtt tgcggggcac gctgcctcgg cgagctgggg
    13981 gattagtacc ggaactgcgg caaatgcagc gatcacgagg gcgcgacgca tgggaacctc
    14041 cttgccaagc gtcagcaaga ctggggcgcg cgggccgatc ccgcattggc gtgcatcaag
    14101 ccggcgccgc aggtgcggcg ggctgtgctg cgaaagtgcc aagtctccgc acttccattg
    14161 ccggcagcct cgctctatgt tgcgccgcca tatgaaggcc cgaggcgtga cacaggggat
    14221 ccccatgcag aagatcaagg tcgcaggcac ggtcgtcgag atggacggcg acgagatgac
    14281 ccgcatcatc tggaaggcca tcaaggacaa gctcatccat ccctacctcg acgtgaagct
    14341 cgagtactac gacctgtctg tcgagatgcg cgacaagacc aacgaccaga tcaccatcga
    14401 cagcgccaac gcgacgaaga agcatggcgt cgccgtgaag tgcgcgacga tcacgccgga
    14461 cgaaggtcgc gtgaaggagt tcaacctcaa ggagatgtgg cgctcgccca acggcaccat
    14521 ccgcaacatc ctcggcggcg tgatcttccg cgagccgatc atctgcaaga acgtgccgcg
    14581 cctggtgcct ggctggacga agcccatcat catcggccgc catgcctacg gcgaccagta
    14641 ccgcgcgacc gacttcaagt tcccaaccaa gggcacgctc tcgatcaagt tcgtcggcga
    14701 cgacggcaag gtgatcgagc gcgaggtgtt caaggcgccg ggcggcggcg tcacgatggc
    14761 aatgtacaac ctcgacgatt cgatccgtga gttcgcgcgc gcctcgatga actacgcgct
    14821 tggccgcgga tatccgctat acctctcgac caagaatacg atcctgaagc agtacgacgg
    14881 ccgcttcatg gagctcttcc aggaggtctt cgacaaggag ttcaaggcga agtacgaggc
    14941 ggccaagatc acctacgagc accgcctcat cgacgacatg gtggcggccg cgctcaagtg
    15001 gtcgggcggc tacgtgtggg cctgcaagaa ctatgacggc gacgtgcagt ccgatacggt
    15061 cgcgcagggc ttcggatcgc tcggcctgat gacttctgtc ctcatgacgc cggacggcaa
    15121 gacggtcgag gccgaggcgg cgcacggcac cgtga
//
LOCUS       DAORNH010000078        15024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3007_length_15024_cov_2.727838, whole genome shotgun sequence.
ACCESSION   DAORNH010000078 DAORNH010000000
VERSION     DAORNH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3007_length_15024_cov_2.727838"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    224..245
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            308..>3068
                     /locus_tag="PK264_09925"
     CDS             308..>3068
                     /locus_tag="PK264_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76244.1"
                     /translation="MTALAAVLLALLIRRSGDFLGRIARTVKKAFTTNWQLTLIASAA
                     FILSCASGYTTWDGMRNFTGEPLLSGMITFGIQGVMLIIAWLIGESFATGMNETSGDR
                     SPAANYLVTLATLFVLALLTTGFLTWVGAIDTALIPGIGETPSQKGAVFLAAAVVAMV
                     LFASLLNNRSSIATTYVATVRVIAKSIMLWVMFLACMATSVFFSFDSLFATIFDKDER
                     KRAADIRAVSQVAGVVADAGGLITRRRAEEAEALFASAGWKRYEETLARVKAAGETAP
                     ALLEQLFKQRAETRLNAVTKHEQVKATAAAQITEFERRRLVLAAEIADLKSKRPAATA
                     AHQAAAAVVAEKRKALDAAKVASEAEARGVEGTGKVGKGPEFRKRMEDLARAQGEYDV
                     AAMRLKDAERPLAEIERRLKTAEPELALVDGEIAKLKATIEAADEQMRIAKETNKSDQ
                     SKFDATTGLEMLRRATLQFRQDPRQETLANIQTLCTTLVSAMSEVPDLKALAKNIDCD
                     PATATEAASRVFALNAGQLAFAATCSGGDKLPQGGGIDKLLDFATRCVQDSGLIGKDT
                     GLLRASLAAINLNRDDKAHRFVVTWNAFLDGNRLAYLALAIAIAIDSLVFMSGLFGAN
                     AVRSPLSDVPSSRPRSAAQLEAVIETALLPDKFENARAALDSMDADTSLPGYMAEVVM
                     SRLDAETRQRVQKVLMAGATINAVRRDPVRPDRYLVRAELYEFLSILSKRAFEEGGAA
                     YRERVEDNLRRADLEKMLTVALLPNIGPGAELVLSHIHPINEVDGYSGEINIAHVPID
                     QQRLVRQVLNAAATARYVRRQQRATTNYEVHTDLYKALARIRGRELLRAKHEAETRPA
                     IAAPAAASVDGGMLTSEVAPSAIAAFAPEPVKPQMATLAPPARGADAFAAPAAMVSPR
                     RAPRGN"
     assembly_gap    3069..3255
                     /estimated_length=187
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3256..3636
                     /locus_tag="PK264_09930"
     CDS             <3256..3636
                     /locus_tag="PK264_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76245.1"
                     /translation="VQAKAREKLQETWKVIFSRQGDQSERIELLAEVFDDIQSRLPLL
                     TLRGMLGGLVAGLEQAAGTDDGLRSDEQSLLDRLREVRIAFETMDPNSEEDWARLGDL
                     VETSSPSDVPPTNVVPFGINKKSV"
     gene            complement(3724..4674)
                     /gene="puuE"
                     /locus_tag="PK264_09935"
                     /pseudo
     CDS             complement(3724..4674)
                     /gene="puuE"
                     /locus_tag="PK264_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
     gene            complement(4709..5149)
                     /locus_tag="PK264_09940"
     CDS             complement(4709..5149)
                     /locus_tag="PK264_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HRD76246.1"
                     /translation="MPVVKGCNLPDNLLYDVDNHIWFDEQPDGTVKLGMTTIATAMAG
                     QLVAFTPKKVGRSVDAGKSCATVESGKWVGPAKSAAAGEVVKINDDLVASPHIANQDP
                     YTQGWFIILRPTDWASVKATLTPGAAVAPKYEAKMQADGFAGCQ"
     gene            5351..>5651
                     /locus_tag="PK264_09945"
     CDS             5351..>5651
                     /locus_tag="PK264_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HRD76247.1"
                     /translation="MTPLTILLISDGRPGHYHLSEGVVAAIARRRFVHVERLEVRRRR
                     SAPGRVLAGLLAMGARRTVLRLGYGLGASQIPAADIVVSAGGDTVAANAAVAGLRG"
     assembly_gap    5652..5688
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5689..6369
                     /locus_tag="PK264_09950"
     CDS             <5689..6369
                     /locus_tag="PK264_09950"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HRD76248.1"
                     /translation="VPEAAVTAVVSSYDRHAGRPRHIVALKPSGIDPDAMPARQAVTL
                     ASPPRRAGLLIGGDSGLFHYEEAEWRQLLDFLDASHAAHGTRWIVSTSRRTSAHVADK
                     AVALARRSGSPIEKLIDYRSAGPGTLPRLFQEVDAILATADSSTMVSEAIAARLPVVG
                     VTPGQSSFKDDEREYRAFMERQGWSRFLPLVGLTPERFLAELAAVRPLAVNPLDRLAT
                     ELAALGIG"
     gene            complement(6373..7233)
                     /locus_tag="PK264_09955"
     CDS             complement(6373..7233)
                     /locus_tag="PK264_09955"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76249.1"
                     /translation="MVDEGRGGSLLSAGALLGPATFMVVLGLLLPMAILFRYSLNIFR
                     PRVMMVDAFSAENYAKFFTDPYYLNVFGTTVRVALLCTVICLILGFPLAYVLARTTTR
                     FKNVLIMLVVLPLFVGNAVRAAGWMTLFGPKGFINVSLEWMGFLKEPLQIMFTEPAVI
                     IGIIAVNLPYMVLTLQSVIEGIERNVEEAAFSLGAGPMVMFRRVLLPLALPGVIAGTI
                     LTFILGMNAYATPVLLGGPKFKMMGPLVFNQFQLNNWPFGAAIAFILMAATLILTALA
                     NLAVQRRYRR"
     gene            complement(7303..8364)
                     /locus_tag="PK264_09960"
     CDS             complement(7303..8364)
                     /locus_tag="PK264_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76250.1"
                     /translation="MTKRDDSKLSRRQVLGATAAIAGSAALGRTTPAKAQAKGRIIVG
                     TWGGDYAKLLNKNIETPHLISKGWEVVQDQAGDPQRRSKLLAEKRLPKGTSDIQGLSA
                     VNMHQMHDAGVIDMIDYAKLSNAPNLLASMKYPYGVGHIYSGKVAVQNNKVVKDPITS
                     YKDVFNPKHGNKLAIIDIQYQYTLVCAALAAGGTPKDLEPAKALLLACKKAGMRIYPT
                     NEAFAAGLKADEFGAGVMWKARVVQWQNAGINVAAITPSEGALAYVSGFVIPKNAPNK
                     EGSYAYMNAMLEKSAQEAFAVDMGYNPTVTTAAVAPDLNKRIGFTPDEIKKLVDLDYP
                     YMTANDVALKEWWDKELKS"
     gene            complement(8406..9479)
                     /locus_tag="PK264_09965"
     CDS             complement(8406..9479)
                     /locus_tag="PK264_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76251.1"
                     /translation="MAGLTLDQVSKRYGDFHAVKRVSLAVPEGEFLVLLGPSGCGKTT
                     TLRIVAGFIEPTEGNVRLGERDVTALPPWKRNAGLVFQSYALFPHLTVAQNVAFGLEM
                     RKVAPGEIGPRVSEALKLVRLGHLGERLPRQLSGGQQQRVALARALVFKPDVLLLDEP
                     LSNLDAKLRQEVRVEIRELQRSLGLTTIMVTHDQEEALTMADRLVVMNEGEVRQLGTQ
                     QDLYERPADRFVAGFVGRSTFLEGTVESAGAFRSLGGLLVHCSGGAPGPASLALRPER
                     LVLTEPGGAFLENAYPGVVEFTSYLGAVLDVHVRLNEQERVIVQIPNRPDGQSPRVGD
                     SVSVGWSRATGSLFAGRSDATGS"
     gene            complement(9479..>9857)
                     /locus_tag="PK264_09970"
     CDS             complement(9479..>9857)
                     /locus_tag="PK264_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD76252.1"
                     /translation="GHVLVVIPWTVRLVTASLAGFDRTIEEAAQSLGANRWTTFRRIT
                     LPAILPGIVAAALFGFVSSFGNLEMSLFLVGPGRTTLPIAILQYLEWKIDPTIAAVSV
                     VQIVLIGAAMLVTDRFVKISRVV"
     assembly_gap    9858..9882
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9883..10287)
                     /locus_tag="PK264_09975"
     CDS             complement(<9883..10287)
                     /locus_tag="PK264_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76253.1"
                     /translation="MSSRWLGRLALKGAAGLGLAYILTPLVFVTWLAFFQQEIPSFPP
                     EGYSLKWFRAIPGNDRFLSGFVLSMQVAVTATLIGLALGVPAALCLARFRFRGREGLN
                     SLLLMPLVVPGIVLGMALYVFHVETQIHTGLEV"
     gene            10592..10774
                     /locus_tag="PK264_09980"
     CDS             10592..10774
                     /locus_tag="PK264_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76254.1"
                     /translation="MSRNQDSPGRLARLVLLPGDLLCNAFGLEGESEHRQVLRSFFNT
                     MIWGAVGIAVTLKLMH"
     gene            10790..11224
                     /locus_tag="PK264_09985"
     CDS             10790..11224
                     /locus_tag="PK264_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRD76255.1"
                     /translation="MPATEFTPWQSLFGGALIGTASVLLMLLHGRIAGLTGIVSGLIP
                     PIAMNDWQWRLALLVGMVSAPATYWLVAGRSPAFHATASVITLVIGGLITGIGVHFSS
                     GCTSGHGVCGLARFSRRSLVAVATFMASTAVTVFVVRHLIGP"
     gene            11221..11685
                     /locus_tag="PK264_09990"
     CDS             11221..11685
                     /locus_tag="PK264_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HRD76256.1"
                     /translation="MTMSSSLTNIVTAALIGLLFGLGIAISGMGNPAKVLAFFDVAGA
                     WDPSLAFVMGGALIVTAVGYRLVFKRNAPVLASRFNVPAGTAIDTPLVAGSAVFGIGW
                     GLTGFCPGGVIPMLGLGRLESLAFFAAFVVGIALARKVRTMLANARRGSVTS"
     gene            11739..13478
                     /gene="sulP"
                     /locus_tag="PK264_09995"
     CDS             11739..13478
                     /gene="sulP"
                     /locus_tag="PK264_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRD76257.1"
                     /translation="MAASNSVQRKLPILGWARSYTRETFSNDLLAAVIVTIMLIPQSL
                     AYAMLAGLPPQVGLYASILPLAAYALFGTGRTLAVGPVAVISLMTASAIGPVSAATGI
                     EPAILAVLLAFLSGGMLLVMGLLRLGLLANFLSHPVISGFITASGILIAAGQLKYILG
                     IKADGDTMLRLVPSLLAGLGATSLATLVIGAGTIAFLFWSRSRLKPLLKANGVQARLA
                     DVLSRAAPVAAIAVTTLATFAFSLDRHGVRIVGAIPSGLPPLTWPSFDIALWRQLAVP
                     ALLISIVGFVESVSVGQTLAARKRLRIDPDQELIGLGAANISASLTGGYPVTGGFARS
                     VVNDDAGAETPAAGVLTAIGIVLAALILTPLLFYLPQATLAATIIVAVLSLVDLGLVG
                     RTWAYSRSDAAAMLSTIAATLSIGVEIGIVTGIVVSLALYLWRASRPHSAVVGLVPGT
                     QHFRNVERHRVITSPEVLTLRIDESLYFTNARYLEDRFADLAADRPALKHVILMCSAV
                     NHIDASALESLEAINRNFKAAGITFHLSEVKGPVMDRLKRSHFLDDLTGRVFLAQFDA
                     IRALDPAVAAKAA"
     gene            13573..14748
                     /locus_tag="PK264_10000"
     CDS             13573..14748
                     /locus_tag="PK264_10000"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRD76258.1"
                     /translation="MTVQGRVAFGAMDEVVFGRPAAEAVAEIARRRRARRVFLMVSGT
                     LNRTTEEIEKVRRALGDSCAGTFDAMPPHTPRAAVIEAAEQARVAQADLIVTVGGGSI
                     TDGAKAVQLCLANDIRIPEAIDRLLPVKGADGAMGPPLSLKAPTVRQISVPTTISGGE
                     FSAIAGVTDERTKVKELIRHPGIMPAAVILDPTITLHTPEWLWLSTGVRAVDHCVEGL
                     CTAEANHFSDAQAVKGLALLASGLPRVKANPADLAARLDCQMGTWLSMGPLAAGVPMG
                     ASHGIGYVLGAAHGVPHGHTSCIMLPAVMRWNRAANSDKQAIVAIAMGEPGREAADVL
                     DRFIAGLGMPRSLSAVKIGRESFGRIAEQAMATPWVPRNPRPIPSPAEVMEILALAS"
BASE COUNT         2387 a         4927 c         4941 g         2498 t
ORIGIN      
        1 gacgggcaag gtcatcggcc gatggtcgtg gggacggtgg cgaatgtgtc ggcgaggtcc
       61 gccttactga agcttaagtt gtcttggacg aggccgctgg gatattcagt aatcacttga
      121 cggagtacta gtccggcgac gggcgcgacg ctccgcaaac gttgagcatc agacgcgtcg
      181 gcgagcgacc agggtcgcga gccaggggta ggcgggttgg cacnnnnnnn nnnnnnnnnn
      241 nnnnnagaag ctcgatggac tacaacttcc tcagctgggg ctttcgcaac agcagatatc
      301 gtggctcttg acagcactcg ccgctgtcct tctcgcgctg ctcatccgtc gctccggcga
      361 tttcctcggg cgcatcgccc gcaccgtgaa gaaggccttc acgaccaact ggcagctcac
      421 gctcattgcc tcggccgcct tcatcctttc gtgcgcctcg ggttacacga cgtgggatgg
      481 aatgagaaac ttcaccggcg agccgctgct ctccggcatg atcaccttcg gcatccaggg
      541 cgtgatgctg atcatcgcgt ggctcatcgg cgagagcttc gcgacgggca tgaacgagac
      601 gagcggcgac cgcagccccg ccgcgaacta cctcgtcaca cttgcgacac tttttgtgct
      661 cgcgctgttg acgaccgggt tcctgacctg ggtcggcgcc atcgatacgg ccctcatccc
      721 ggggatcggc gagacaccga gccagaaggg cgccgtattc ctcgccgccg ccgtggtcgc
      781 catggtgctg ttcgcgagcc tcctcaacaa ccgctcgagc atcgccacga cctacgtcgc
      841 gaccgtgcgc gttatcgcga agagcatcat gctctgggtc atgttcctgg cctgtatggc
      901 cacgagcgtg ttcttctcct tcgacagtct tttcgccacg atcttcgaca aggacgagcg
      961 caagcgcgcc gccgacatcc gcgcggtgag tcaggtcgcg ggcgtcgtcg cggatgccgg
     1021 cggcctcatc accaggcgcc gcgccgagga ggcggaagcg ctgttcgcaa gcgccggctg
     1081 gaagcgctac gaggagacgc ttgcacgggt gaaggctgcc ggcgagacgg cgccggccct
     1141 tctcgagcag ctcttcaagc agcgcgccga gacgcgcctc aacgccgtta ccaagcacga
     1201 gcaggtgaag gcgaccgccg cagcccagat caccgagttc gagcggcggc gcctggtgct
     1261 tgccgccgag atcgccgacc tcaagtcgaa gcggccggct gcaacggccg cgcaccaggc
     1321 ggcggcggcc gtggtcgccg agaagcgcaa ggcgctcgat gccgccaagg tcgcctccga
     1381 ggcggaggcg cgcggcgtcg agggcaccgg caaggtcggc aagggccccg aattccggaa
     1441 gcgcatggag gatctcgccc gcgcccaggg cgaatatgac gtcgccgcca tgcggctcaa
     1501 ggatgccgag cgaccgcttg ccgagatcga gcgccgcctc aagacggccg aacctgaact
     1561 cgcgctcgtc gacggcgaga tcgccaagct caaggccacc atcgaggcgg ccgacgagca
     1621 gatgcggatc gccaaggaga ccaacaaatc cgaccagtcg aagttcgatg ccacgaccgg
     1681 cctcgagatg ctgcgacgcg cgacgctgca gttccgccag gacccgaggc aggagacgct
     1741 tgccaacatc cagacgctct gcacgacgct cgtctcggcg atgagcgagg tgccggatct
     1801 caaggcgctg gccaagaaca tcgattgcga tccggcgacg gcgacggagg cggcctcgcg
     1861 cgtgttcgcg ttgaacgcgg gccagctcgc gttcgcggca acctgctctg gcggcgacaa
     1921 gcttccgcag ggcgggggca tcgacaagct cctggatttc gcgacgcgct gcgtgcagga
     1981 cagcggcctc atcggcaagg acacggggct gctgcgcgcc tcgctcgccg cgatcaacct
     2041 gaaccgcgat gacaaggcgc atcgcttcgt cgtcacctgg aacgccttcc tcgatggcaa
     2101 ccggctcgcc tacctggcac tcgccatcgc catcgcgatc gacagcctcg tcttcatgtc
     2161 gggtctcttc ggcgcgaacg ccgtccgctc gccgctctcc gacgtgccga gttcgcgccc
     2221 ccgctcggcc gcccagctcg aggccgtgat cgagaccgcg ctgctgcctg acaagttcga
     2281 gaacgcgcgc gccgccctcg attcgatgga tgccgatacc tcactccccg gttacatggc
     2341 cgaagtggtg atgagccgcc tcgacgccga gacgcgccag cgcgtgcaga aggtgctgat
     2401 ggccggcgcc accatcaacg ccgtgaggcg cgatcccgtg cggcccgatc gctacctcgt
     2461 gcgcgccgag ctctacgagt tcctgtcgat cctctcgaag cgcgctttcg aggagggcgg
     2521 cgcggcctac cgcgagcgcg tcgaggacaa cctgcgccgc gccgacctcg agaagatgct
     2581 gacggttgcg ctgctcccca acatcggccc cggcgctgag ttggtgctga gccatatcca
     2641 cccgatcaac gaggtcgatg gctactcggg cgagatcaac atcgcccacg tgccgatcga
     2701 tcagcagcgc ctcgtccgcc aggtgctgaa cgcggcggcc accgcgcgct atgtccgccg
     2761 ccagcagcgt gcgacgacca actacgaggt ccacaccgac ctctacaagg cgcttgcgcg
     2821 catccgcgga cgcgagctct tgcgcgccaa gcacgaggca gaaacacggc cggccatcgc
     2881 ggcacccgca gccgcaagcg tcgacggcgg catgctgacc tcggaggtcg ccccttccgc
     2941 gattgcggct ttcgccccgg agcccgtcaa gccgcagatg gcaacgctcg cgccgccggc
     3001 gcgcggagcc gacgcgttcg cagctccagc agcgatggta tcgccaaggc gcgcaccgcg
     3061 tggcaacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnngtgca ggccaaggcg cgcgaaaagc tgcaggagac ctggaaggtg
     3301 atcttctcgc gccagggcga ccagagcgag cggatcgaac tcctcgccga ggtcttcgac
     3361 gacatccaga gccgcctgcc gctgctcacc ttgcgcggca tgctcggcgg cctcgtcgca
     3421 ggactcgagc aggcggccgg gaccgacgat ggccttcgct cggacgagca aagcctgctc
     3481 gatcggctgc gcgaggtgcg catcgccttc gagacgatgg atccgaacag cgaagaggat
     3541 tgggcgcggc tcggcgatct tgtagagacc tcgagcccga gcgacgtgcc gccgaccaat
     3601 gtcgtgccgt tcggtatcaa caagaagagc gtttgaggag cgtcagcccc ggcgtcgatg
     3661 tcggccgacc ctggccgacc ctggccgacc ctggccgaca gcggctgatc aggcccgcgg
     3721 cttcggcggg tgcaccttgt gccaatgctc ggcgatgtcg atgcggcgcg ccacccagac
     3781 gcggtcgtgg gccagcacgt agtcgaggaa gcgctcgagc gctgcgaccc gacccggccg
     3841 gccggcgagc cggcagtgca ggccgatcga cagcatcttc ggtgcctcgg cgccctcacg
     3901 gtagagcacg tcgaaggaat ccttcagata ggtgaagaac tgatcgcccg cattgaagcc
     3961 ttgcggcgtc gcaaagcgca tgtcgttggc atcgagcgtg tacggcacga tcaagaacgg
     4021 ctctttcggt cccgtgaccc agtagggcag atcgtcggcg tagctgtccg ccgagtagcg
     4081 gaacccgccc tccgccatga cgattgcgag cgtgttggcc gagcagcgcc cctggtagaa
     4141 gccgagcggc cgctctcccg caatctcggt gtggaggcgg acggcctcga ggatctgcgc
     4201 ccgctcctcg gcctcgccca tgtccttgta gtcgatccac ttgaggccat gcgtggcgag
     4261 ctcccagccc gcctcctgca tggcggcgac ctgctcgcgg ccgcgcatca tggcgtgtgc
     4321 gacggcaaag gccgtcacgg gcacgcggcg cgacgtgaac gcccgccaga gccgccagaa
     4381 gcccgcgcgc gcgccgtatt cgtagatcga ttccatattc atgtgccgct ggccgggcca
     4441 gggctgcgcg ccgacgatct cggagaggaa tgcctccgag gcctggtccc cgtgcaggat
     4501 gcagttctcg ccgccctcct cccagttgat cacgaactgc acggcgatgc gggcgcggcc
     4561 gggccattgc gggtccggcg gcgtccgccc gtaaccgacg agatcgcgcg gataggcctc
     4621 ggtgaccggc gctcggcccg caggcccaga cacgcgtggc ttgcgcggcg gcatgtcgac
     4681 ctcgcgaaga gccttcgggg acgtaagcct actggcagcc ggcgaagccg tcggcctgca
     4741 tcttggcctc atacttcggt gcgacggcgg caccgggcgt gagcgtcgcc ttgaccgacg
     4801 cccagtcggt cggcctcagg atgatgaacc agccttgcgt gtaggggtcc tgattggcga
     4861 tatgcgggct cgcgacgagg tcgtcgttga tcttcacgac ctcgcctgcc gcagcgctct
     4921 tggccggtcc cacccatttg ccgctctcga ctgtcgcgca gctcttgccg gcgtcgacgc
     4981 tgcgaccgac cttcttcggc gtgaaggcaa cgagctggcc agccatcgcc gtcgcaatcg
     5041 tcgtcatgcc gagcttgacg gtgccgtccg gctgctcgtc gaaccagatg tggttgtcga
     5101 catcgtagag gaggttgtcg gggaggttgc aacctttgac gacgggcatg ggtgtgctct
     5161 gctgccgaac ggtttattcg aatgcctgca tatgatgatc gctcgccgcc gtgggtgcaa
     5221 gctcaaatcc tcctccgaag cggccatcga cggggatgat cgtcgcaggc gtgccaccgc
     5281 gacgaagagc agcgtggcaa ggctggcgtc gaaggcttaa tcggagcctt ccctacctgc
     5341 gacgagcctg atgacgccgc ttacgatcct cctcatctcc gacggtcgac ccggccacta
     5401 tcacctcagc gaaggcgtgg tggcggcgat cgcgcgccgc cggttcgtcc acgtcgagcg
     5461 cctcgaggtc cgacggcggc ggagtgcccc cggacgcgtg ctcgccggcc tgctcgccat
     5521 gggggcgcgg cggaccgttc tcaggctcgg atacgggctc ggtgcaagcc agatcccggc
     5581 cgccgacatc gtcgtctcgg ctggcggcga tacggtcgcc gccaatgctg ccgtggcagg
     5641 cctcagaggc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tccggaggcg
     5701 gccgtgacgg cggtcgtctc ctcctacgat cgccatgctg gccgcccccg ccatatcgtc
     5761 gcactgaagc cgtcaggcat cgatccagac gccatgccgg cccggcaggc agtgacgctc
     5821 gcttcgccgc cgcgccgcgc ggggctgctg atcggcggcg attccggcct ctttcactat
     5881 gaggaggcgg agtggcgcca gctgctcgac tttctcgatg cctcgcacgc ggcccatggc
     5941 acacgctgga tcgtctcgac gtcacggcgt acgtccgccc acgttgccga caaggccgtc
     6001 gcgctcgccc gtcggtcggg aagcccgatc gaaaaactga tcgactatcg gagcgcaggg
     6061 ccgggcacgc tgccgcgcct cttccaggag gtcgatgcga tcctcgccac cgccgacagc
     6121 tcgaccatgg tgtcggaggc gatcgccgcg cggctgccgg tcgtcggcgt cacgcccggg
     6181 cagtcgagct tcaaggacga tgagcgcgag tatcgcgcct tcatggaacg tcagggctgg
     6241 agccgcttcc tgccgcttgt cgggttgacg cctgagcgct tcctcgccga gctcgccgcc
     6301 gtgcgaccgc ttgcggtcaa tccgctggac cggctcgcga ccgagctcgc ggcgctgggg
     6361 atcggatagc gctcagcgcc ggtagcggcg ctgcacggcg agattggcga gcgcagtcag
     6421 gatcagcgtc gccgccatca ggatgaaggc gatcgctgcg ccgaatggcc agttgttcag
     6481 ttggaactgg ttgaagacga gcggccccat catcttgaat ttgggcccgc cgagcagcac
     6541 cggcgtcgca taggcgttca tgccgaggat gaacgtcagg atcgtgccgg cgatcacgcc
     6601 cggcagcgcg agcggcagca gcacgcggcg gaacatcacc atcggccccg cgccgagcga
     6661 gaaggccgct tcctcgacgt tacgctcgat cccttcgatc acgctctgca gggtcagcac
     6721 catgtaggga aggttcaccg cgatgatgcc gatgatcacc gcgggctcag tgaacatgat
     6781 ctgcagcggc tccttgagga agcccatcca ctcgagcgac acattgatga agcccttcgg
     6841 tccgaacagc gtcatccagc cggccgcgcg caccgcattg ccgacgaaga gcggcaacac
     6901 gacgagcatg atcagcacat tcttgaagcg cgtcgtcgtg cgggcgagca cataggcgag
     6961 agggaagccg agtatgaggc agatgaccgt gcacagtagt gccacgcgga ccgtcgtgcc
     7021 gaacacgttc aggtagtagg ggtcggtgaa gaacttggcg tagttctcgg ccgagaacgc
     7081 gtcgaccatc atcacgcgcg gacggaagat attgaggctg tagcggaaga ggattgccat
     7141 cggcagcaga agcccgagca cgaccatgaa ggtcgctggg ccgagaagcg cgcccgccga
     7201 gagcagcgag ccgcctcgcc cctcatcgac catgccgcgt gctggttgtg ctgctgccgc
     7261 catggcgcat catgggccgg cgttgctgcc ggcccgctcc gatcaggact tgagctcctt
     7321 gtcccaccac tccttcaagg cgacgtcgtt cgccgtcatg tatgggtagt cgagatcgac
     7381 gagcttcttg atctcgtcgg gtgtgaagcc gatgcgcttg ttgaggtcgg gcgcgacggc
     7441 agcggtggtg acggtcgggt tgtagcccat gtcgaccgcg aaggcctcct gcgcgctctt
     7501 ctcgagcatg gcgttcatgt aggcgtagga gccctccttg ttcggcgcgt tcttcgggat
     7561 aacgaagccc gagacatagg cgagagcgcc ctcgctcggc gtgatcgcgg caacgttgat
     7621 gccggcgttc tgccattgga cgacgcgtgc cttccacatc acgccggcac cgaactcgtc
     7681 cgccttgaga ccggcggcga aggcctcgtt cgtcgggtag atgcgcatgc cggccttctt
     7741 gcaggcgagc agcagcgcct tggcgggctc gaggtctttc ggtgtgccgc ctgcagcaag
     7801 ggccgcgcac accagcgtgt actggtactg aatgtcgatg atggcgagct tgttgccgtg
     7861 cttcggattg aacacgtcct tgtagctcgt gatcgggtcc ttcacgacct tgttgttctg
     7921 gacggcgacc ttgcccgagt agatgtggcc gacgccgtaa ggatacttca tcgaggcgag
     7981 cagattgggc gcattcgaga gcttggcgta gtcgatcatg tcgatgacgc cggcgtcatg
     8041 catctggtgc atgttgacgg cggagaggcc ctggatgtcc gaggtgccct tcggcaggcg
     8101 cttctcggcg agcagcttgg agcggcgctg tgggtcgccg gcctggtcct gcacgacctc
     8161 ccagcctttc gagatgaggt gcggcgtctc gatgttcttg ttgaggagct tggcgtagtc
     8221 gccgccccag gtgccgacga tgatgcggcc ctttgcctgg gccttggcgg gtgtggtacg
     8281 accgagtgcc gcgctgccgg cgatggcggc cgtggcacca agcacctggc gccgtgaaag
     8341 cttgctgtcg tctcgcttgg tcatgcgttt cctccgattg agcttgattg cgtctatgcg
     8401 tctgctcaag atccggtcgc gtcactgcgc ccggcaaaca gcgatcccgt cgcccggctc
     8461 cagccgacac tgacactatc gcctactctg gggctttggc catcgggccg attgggaatc
     8521 tggacgatga cgcgctcctg ctcgttcagg cggacgtgaa cgtcgagcac tgcaccgaga
     8581 taggatgtga actcgacgac gccggggtag gcgttttcga gaaacgcgcc tccgggctcg
     8641 gtgagcacca gccgttcggg acggagggcg agtgaggcag gccccggtgc gccgcccgag
     8701 cagtggacga gcagcccgcc cagtgaacgg aaagctccag ccgattcgac ggtgccctcg
     8761 agaaaggtcg agcggccgac gaagcccgca acgaagcggt ccgccggtcg ctcgtagagg
     8821 tcctgctgcg tgccgagttg gcgcacctcg ccctcgttca tcacgacaag gcggtcggcc
     8881 atggtcagcg cctcctcctg gtcgtgcgtc accatgatcg tggtgaggcc gagcgagcgc
     8941 tgcaactcgc ggatctcgac gcgcacctct tggcggagct tggcatcgag gttcgaaagc
     9001 ggttcgtcga gaagcagcac gtccggcttg aagacgaggg cgcgggcaag cgccacgcgt
     9061 tgctgctggc cgccagagag ctggcgcggc aggcgttcgc cgaggtggcc gagccggacg
     9121 agcttcaagg cctcggaaac gcgggggccg atctcgccgg gagcgacctt gcgcatctcg
     9181 aggccgaagg cgacgttttg cgcgaccgtc aggtgcggaa agagtgcgta gctctggaag
     9241 acgagcccgg cattgcgctt ccacggcggc agcgctgtca catcgcgttc gccgagacga
     9301 acgtttcctt ctgtcggctc gatgaagccg gcgacgatcc tgagcgtcgt cgtcttgccg
     9361 cagcccgagg gcccgagcag gacgaggaac tcgccctcgg gcacggcgag cgacacgcgc
     9421 ttcacggcat ggaaatcgcc gtagcgcttg ctcacttggt cgagtgtcag gcccgccatc
     9481 agaccaccct gctgattttc acgaagcggt cagtgactag catggccgcc ccgatgagca
     9541 cgatctggac gaccgagacg gccgcgatcg tcgggtcgat cttccattcg agatactgga
     9601 ggatggcgat cggcagcgtg gtgcggccgg gaccgacgag gaacaggctc atctcgagat
     9661 tgccgaacga ggagacgaag ccgaacaggg cggcggcgac gattcccggc aggatcgcgg
     9721 gcaacgtgat gcgccggaac gtcgtccagc gattggcacc gaggctctgc gccgcctcct
     9781 cgatggtgcg gtcgaagccg gcgaggctcg cggtgacgag gcgcacggtc caggggataa
     9841 cgaccagcac atgtccgnnn nnnnnnnnnn nnnnnnnnnn nncacctcga ggccggtgtg
     9901 gatctgcgtc tcgacgtgga acacgtagag cgccatgccg agaacgatcc ccggcacgac
     9961 gagcggcatg agcaggaggc tgttcagccc ctcgcggccg cgaaagcgga accgggcgag
    10021 gcagagtgcc gccggtacgc cgagagcgag gccgatgagg gtcgcggtga cggcgacctg
    10081 catgctcagc acgaagccgg agaggaagcg atcatttccc ggaatcgcgc ggaaccactt
    10141 gagcgagtag ccctcgggcg ggaacgaggg gatctcctgc tggaagaacg ctagccaggt
    10201 gacgaagacg agcggtgtca ggatgtaggc cagtcccagg cctgctgcgc ccttaagcgc
    10261 gagccggcca agccatctcg aactcatatg acctgagagt gatgccaccc cacccatccc
    10321 cacgcgccgt tgctctcggc cgatgttctc gaatgtcatc cgcgaaggcg gggccccgag
    10381 ccgtcgagcc cgaccagcat cgcatatccg aggcagcgtg caagcgccgt atttcgggca
    10441 aggccgagcc tggtttcccg gcagtgacgg gcgacggctg cgtcaaagag atgctggcgg
    10501 gtcggttgct gcgccagcga tcggtgacgt agtcacatat cggacggagc agatcgcgta
    10561 gcaacgggtc gatccagatc aaggaacccg catgtcacgc aatcaggaca gtccaggccg
    10621 gctggctcgc ctcgttcttc taccgggcga cctcctctgc aacgccttcg gcctcgaggg
    10681 cgagagcgag catcgccagg ttctgcgcag cttcttcaat acgatgatct ggggtgctgt
    10741 cggcatcgcc gtcaccctca agctcatgca ctgatctcag ggaggcacga tgccggctac
    10801 cgaattcacc ccgtggcagt cgctgttcgg cggcgcgctc atcggcacgg cgtccgtgct
    10861 cctcatgctg ctgcatggac gcatcgccgg gctcacgggc atcgtgtcgg ggcttatacc
    10921 gccgatcgcc atgaatgact ggcagtggcg cctcgctctc ctcgtcggga tggtctcagc
    10981 gcccgccaca tactggcttg ttgccggccg gagccccgca ttccatgcga cggcgtccgt
    11041 gatcacactt gtgatcgggg gcctcatcac cggcatcggc gtgcatttca gctcgggctg
    11101 cacgagcgga cacggggtct gcggtctcgc ccgcttctcg cgccgctccc tggtcgcggt
    11161 cgcaaccttc atggcatcga ccgcggtgac ggtcttcgtc gtccgccacc tgatcgggcc
    11221 atgaccatgt cctcgagcct caccaacatc gtcaccgcgg ccctgatcgg cctgctcttc
    11281 gggctcggga tcgcgatctc agggatgggc aatcccgcca aggtcctcgc cttcttcgac
    11341 gtcgccggcg cctgggatcc gagccttgcc tttgtgatgg ggggcgcgct gatcgtcacg
    11401 gccgtcgggt atcgcctcgt cttcaagcgg aatgcgccgg tgctcgcgtc gcgcttcaac
    11461 gttccggccg gcacggcgat cgacacgccg ctcgtcgctg gatcggccgt tttcgggatc
    11521 ggttggggat tgaccgggtt ctgccccggc ggcgtcattc ccatgctggg cctcggccgc
    11581 ctcgagtcgc tcgccttctt tgcggcgttc gtggtcggga tcgccttggc tcgaaaagtc
    11641 cgaacgatgc ttgccaacgc tcgtcggggc agcgtcacgt catgaaggtg ccaggccgcc
    11701 acgagatcac tcgcgtgtcc gcacgcctgc tcgcgacaat ggccgcttca aactcggtgc
    11761 agcgcaaact gccgattctc ggctgggccc gcagctacac ccgcgagaca ttctcgaacg
    11821 acctgctcgc ggcagtaatc gtcaccatta tgctgatccc gcaaagcctc gcctatgcga
    11881 tgctggcggg cctccctccg caggttgggc tctacgcgag catcctgccg ctcgccgcct
    11941 atgccctctt cggcacggga cgcacactcg ccgtcgggcc ggtggccgtc atctcgctga
    12001 tgacggcctc ggcgatcggt ccggtgagcg ccgcgaccgg tatcgagccg gcgatcctgg
    12061 cggtattgct cgccttcctg tccggcggca tgctgctcgt catggggctg ctgcggctgg
    12121 gattgctcgc gaatttcctg agccatcccg tcatttcggg tttcatcacg gcatctggca
    12181 tcctgattgc cgcgggccag ctcaagtaca tcctcggcat caaggccgac ggcgacacga
    12241 tgctgcggct cgtgccctcg ctccttgcgg ggctcggcgc gacgagcctc gcgaccctcg
    12301 tcatcggggc gggcacgatc gccttcctgt tctggagccg cagccgactg aagccgctcc
    12361 tcaaggcgaa tggggtccag gcgcggcttg ccgatgtgct gagccgcgcg gcgcccgtgg
    12421 cggccattgc ggtgacgacg ctcgcgacgt tcgccttctc gctcgatcgg cacggcgtga
    12481 ggatcgtcgg cgccatcccg agcgggctgc cgccgctcac ctggccgagc ttcgacatcg
    12541 cgctctggcg gcagcttgcc gtacctgcat tgctgatcag catcgtcggc ttcgtcgaat
    12601 ccgtctccgt cggccagacg ctcgccgcgc gcaagcgcct gcgcatcgat cccgaccagg
    12661 agctgatcgg gctcggcgcg gccaatattt cggcgtccct gacgggcggt tatcctgtca
    12721 ccggcggctt tgcgcgctcg gtcgtcaatg acgacgcggg cgccgagacc cccgccgccg
    12781 gagtgctgac agccattggc atcgtgctcg cggccctgat cctgaccccg cttctcttct
    12841 atctgccgca ggcgacgctc gccgcaacca tcatcgtcgc ggtcctctcg ctcgtcgatc
    12901 tcggtctggt cgggcggacc tgggcctatt cccgcagcga cgcggccgcg atgctctcga
    12961 cgatcgccgc aacgctttcg atcggcgtcg agatcggcat cgtgaccggc atcgtcgtct
    13021 cgctggcact ctacctctgg cgggcgagcc ggccgcacag tgccgtcgtc gggctcgtcc
    13081 ccggcacgca gcacttccgc aacgtggagc ggcaccgcgt gatcaccagc cccgaggttc
    13141 tcactctgcg aatcgacgag agcctctact tcacgaacgc ccgctacctc gaggatcgct
    13201 tcgccgacct tgccgccgat cggccggcac tcaagcacgt catcctgatg tgctccgcgg
    13261 tcaaccatat cgacgcgagt gcgctcgaga gcctcgaggc catcaatcgc aatttcaagg
    13321 ctgcagggat cacgttccat ctgtcggaag tcaagggacc ggtgatggac cgcctgaagc
    13381 gatcacattt tctcgatgat ctgacgggcc gcgtgttcct cgcgcagttc gatgcgatcc
    13441 gcgccctcga ccccgccgtc gcggcgaaag cggcctgaga gccgcctgag ggcgggcgct
    13501 tgccgcgacg cagctcgcct gccagagtga cagcgggaga caggccgcgc gtcaaagcgc
    13561 acgggggaaa ccatgacggt acaagggcgg gtcgcgttcg gggccatgga cgaggttgta
    13621 ttcggccggc cggcggccga ggctgtcgcc gagatcgcaa ggcggcgcag ggcgcggcgc
    13681 gtcttcctca tggtgagcgg tacgctcaac cggacgacgg aggaaatcga gaaggtccgc
    13741 cgggcgctcg gcgacagctg tgccggcacg ttcgatgcga tgccgccgca tacgccgcga
    13801 gccgccgtga tcgaggcggc cgagcaggct cgtgtcgccc aggctgacct catcgtcacg
    13861 gtcggcggcg gctcgatcac ggatggcgcc aaggctgtcc agctctgcct cgccaacgat
    13921 attcgcatcc ccgaggcgat tgaccggctc ctgccggtga aaggcgcaga cggtgcgatg
    13981 ggcccgccgc tctccctcaa ggcgccgacg gtgcgccaga tcagcgtgcc gaccacgatc
    14041 tcgggcggcg agttcagcgc cattgccggc gtcacggacg agcgaactaa ggtgaaggag
    14101 ttgatccggc atcccggcat catgccggcg gcggtcatcc tcgatccgac gatcacgttg
    14161 cacacgcccg agtggctgtg gctgtcgacg ggtgtgcgcg cggtcgatca ctgcgtcgag
    14221 gggctctgca cggccgaggc caaccatttc tcggacgcgc aggccgtcaa gggtctggcg
    14281 ctgctcgcga gcgggctgcc gcgtgtcaag gccaatcctg ccgatctcgc cgcgcgcctc
    14341 gattgccaga tgggcacgtg gctttcgatg gggccgctcg cggccggcgt gccgatgggc
    14401 gcgagccacg gcatcggcta tgtgctcggt gcggctcatg gcgtcccgca cggccacacc
    14461 tcgtgcatca tgctgccggc cgtgatgcgc tggaacaggg cggcgaactc ggacaagcag
    14521 gcgatcgtcg ccatcgccat gggcgaaccc gggcgcgagg ccgccgacgt gctcgatcgc
    14581 ttcatcgccg ggctcggcat gccgcgcagc ctctcggccg tgaagatcgg ccgcgagagc
    14641 ttcggccgga tcgccgagca ggcaatggcg acgccgtggg tgccgcgcaa tccgcgccca
    14701 attccgtcac cggccgaagt catggagatc ctggcgctcg cgtcctgatc ttcgcccgga
    14761 acaacagtta tcgtcgcgaa gatcgcgccc cgctattgta ttggtggggt ggggggccca
    14821 tgacgcggca ccaatgactg ctcaaccggt gaaggagacg gccatgttcg atgccaggca
    14881 gctgatcgag atgatgatgg gcggcgccgg gcagggcgcg ggccgcgggc agccagcgca
    14941 acccgcgggc ggcatgggtg ggctcggcga tatcctcggc caggtgctcg gcggcggaca
    15001 gccccagccc ggccgcggcg acca
//
LOCUS       DAORNH010000079        14987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3021_length_14987_cov_6.545205, whole genome shotgun sequence.
ACCESSION   DAORNH010000079 DAORNH010000000
VERSION     DAORNH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3021_length_14987_cov_6.545205"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..449)
                     /locus_tag="PK264_10005"
     CDS             complement(114..449)
                     /locus_tag="PK264_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HRD76259.1"
                     /translation="MKNYVQPGNTITLAAPYAVASGDGLLVGAIFGVATAAAANAEPV
                     EAALVGVFDLKKVGSQAWAAGDKIYWDNTAKETTKTATANTLIGVAVEAVANGAADIV
                     GRVRLNASF"
     gene            complement(527..2476)
                     /locus_tag="PK264_10010"
     CDS             complement(527..2476)
                     /locus_tag="PK264_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRD76260.1"
                     /translation="MHGTIELPAMRRAADLLPATLDEQDRSIEVVWSTGARVRRQPLF
                     GEPFDEELSMDPGSVRLERLNAGGPLLKVHDLRTLDSVIGSVVPGTARIDSGRGIARV
                     RFSERDDVEPIWADVRAGHLRAVSIGYQVHRFEVSRPANAPEVWRAVDWTPFEISAVP
                     VGADPAAGFRSVDPLIPCVVDRDDASQQMRTSMEETNVTPTPAQATPEPITRAADPQP
                     DTQALIANAQTAERERVGTIYDLASRLGLERSLAEDLVTRGVAIDEARRVILDKVADT
                     AEKTRTFPHVSVPLGGRDERLTRREAVANALLHRYSPTLFGLSDPAREYRGMTLLELS
                     REFLASAGVNVRGMSRDEIATRALHSTSDFPEVLAAVTNKTLRQAYDVYPRTFVPFCR
                     QVLATDFKAMNRVQLGEAPQLLKVSEGGEFKRGTISESKESYRIETYGRVVAITRQVL
                     INDDLDAFTRIPAMYGTAIATLESDVVWGIVTANAAMADGVALFHATHKNLAGTGAAL
                     SVAAVGEGRASMAKQTGLDKKTVLNIRPSYLVVPAALELAAEQLIAQNLVPAKTGDVV
                     PQSIRTLTPISEPRLDAVSTGAWYLAANPAQIDTIEYAYLEGQQGAYIETRNGFDVDG
                     VEIKCRLDFGAKAIDWRGLYKNPGA"
     gene            complement(2487..3974)
                     /locus_tag="PK264_10015"
     CDS             complement(2487..3974)
                     /locus_tag="PK264_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRD76261.1"
                     /translation="MRRVSWLDRAIGSVAPRAALRRVQARESFDALARGYDGAAKGRR
                     TDGWRVAGTSADSEIAVAGGLLRDRMRDLVRNNPHAAKAVSVLVNNIIGSGIIARAAS
                     GNDKLDSQVNALWEAWSALCDADGQLDFLGLQTLACRQMIEAGEVLIRRRPRRASDGL
                     DVPLQLQLLEADMLDAGRNGDLADGGRVVQGIEFSSLGQRRAFWLFAQHPGDSVVTTR
                     RRLDSLAIPAADIVHLYEKQRMQVRGVPWGTPVMRALRDLDDWTQAELVRKKTEACVV
                     GIVLGADEAEQGVAPSVVDADGNRVEQFEPGLIAYARGGKDIRFNQPATTAGVAEWLR
                     AQLHIVAAGFRMPYELLTGDLSQVNYSSIRAGLVEFRRLIDAVQWQIVIPMLCQPTWD
                     WFTEQAWAAGKLPQPRIPVEWSPPRFEAVDPLKDAMADLLAMRSGTMTLAQAIARQGH
                     NPDAVLAEIAAMNAKIDALGLIFDSDPRRVTKTGVMQPDPGLTST"
     gene            complement(3974..4183)
                     /gene="gpW"
                     /locus_tag="PK264_10020"
     CDS             complement(3974..4183)
                     /gene="gpW"
                     /locus_tag="PK264_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HRD76262.1"
                     /translation="MSWTTAELDALRRAYASGTLRVSYDGKTVEYGSAADLLSRIRTI
                     EREIAGAAENRPPVAGFAGFSRGER"
     gene            complement(4180..6201)
                     /locus_tag="PK264_10025"
     CDS             complement(4180..6201)
                     /locus_tag="PK264_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRD76263.1"
                     /translation="MSAPTSPNLPRSDQSSVDLFGFEGAEELWQSWRDGLTPDPLLTV
                     SEWADRHRFLSPRASAEPGRYRTDRTPYMRAIMDALSPSHPARRIVFMKAAQVGATEA
                     GNNWIGYVIHHAPGPMLSVQPTVELAKRFSRQRIEPLIAESPALRERVKPARARDAGN
                     TVLSKEFPAGLLVITGANSAVGLRSMPARYLFLDEVDAYPPSADEEGDPVALAEARTR
                     TFSWRSKVLLASTPTIYGVSRIEREFDASDQRRFFVPCPHCQHRQWLRFERLRWEKGK
                     PDSAHYQCEACDGEIEEHHKIEMLHAGDWRPTAEAADPGTIGFHLSALYSPVGWMSWA
                     MIARMWEASLATNEAKRSFKNGVLGETWIETGEAPDWQRLYERREDWQIGTVPSGGLF
                     LTAGADVQKDRIEVSVWAWGRGLTSWFVDHIVIDGGPEHAETWSQLSGLLDRTWPHAH
                     GARLGLAKLGIDSGYESPAVYAWARSAGHAQVVPLKGVEGFNRAAPVVGPSFVDVTEA
                     GRKLRRGARLWTVAVATFKSETYRHLRLTRPTDEEIAEGAQYPAGFVHLPRGLEAEWV
                     KQLVAEQLVSAKTRRGFQRLEWQKLRERNEVLDCRVYARAAAWIAGADRWTDEKWRDL
                     EDQVGPVPEGIVDVKADASIAAGVLARAPVAGGKRRSDWLSGVDKGWLR"
     gene            complement(6149..6694)
                     /locus_tag="PK264_10030"
     CDS             complement(6149..6694)
                     /locus_tag="PK264_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elements of external origin"
                     /protein_id="HRD76264.1"
                     /translation="MGLSIRAYARHRGVSHVAVLRAAKAGRVSLEPDGTIDPAKADIS
                     WERSTEPGRSRAKPEKLKPVAEAAMGSVRETLKEQGLPASGNVTFVQARTAHEIAKAH
                     LARLRLQRMKGELIDRARATALVFRMAREERDTWVNWPARVAALIAAELGVEAHPMQK
                     ALETHVRAHLAELAEVRPEFR"
     gene            6799..7020
                     /locus_tag="PK264_10035"
     CDS             6799..7020
                     /locus_tag="PK264_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76265.1"
                     /translation="MTKTPLKQTALDAFIARKAEIDAMLDRLKSLSDDHFDYAPDDID
                     WGHVGTLAHYAELLKRITDSAFKEGEHAE"
     gene            7123..7716
                     /locus_tag="PK264_10040"
     CDS             7123..7716
                     /locus_tag="PK264_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HRD76266.1"
                     /translation="MAKLSDSQLVVLSTACQRPDGCVFPVTLKLKGNAVGNVLKSLLT
                     KGFLEEVPGRADDTVWRYDDGAPLTLQASRAAYDELGIEPETDPATEGRHAAAIDDCG
                     LVSGSPAEQGIVAAKIETRARKTDQAPRTRGDSKQAKLIEMLKRPEGASIEEIVAAFD
                     WQAHTVRGAIAGALKKKLGLDVTSEKIDGRGRVYRIA"
     gene            complement(7745..7990)
                     /locus_tag="PK264_10045"
     CDS             complement(7745..7990)
                     /locus_tag="PK264_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76267.1"
                     /translation="MNKQSRRMSLVESIANVVVGFGIAVLTQIAVFPIFGLQVSLADN
                     LVIGLVFTFVSIARSFALRRVFEEFRVRAERKSAVRL"
     gene            complement(7987..9237)
                     /locus_tag="PK264_10050"
     CDS             complement(7987..9237)
                     /locus_tag="PK264_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD76268.1"
                     /translation="MQRDHIQIEQWPLDRLLPYAANARTHPDEQVAQIAGSIAEFGFN
                     VPCLVDERGVLVAGHGRLLAAQRLGLSQVPVIRLDHLTDAQARAYRIADNQIALNSGW
                     DDALLSAEVARLKEDGVDLELLGFPEDELDRLLAGLDGEKGGNEDEDVVPEPPETPIS
                     RPGDLWILGRHRLLCGDATCASDVARLVGDVRPHLMVTDPPYGVDYDPSWRNDAGVSA
                     TARTGRVSNDDRADWREAWALFPGDVAYVWHAGVHARTVAESLDASGFPIRSQIIWSK
                     SRFVLGRGDYHWQHEPCFYAVRKGATGHWQGARDQSTVWTIGGGGDEDAATVHGTQKP
                     VECMRRPMVNNSERGDAVYEPFAGSGTTLIAAESIERRCFAMEIDPRYCDVIIERWQS
                     FTGSKAQLETDGRSVEDVRSERGA"
     gene            complement(9227..10609)
                     /locus_tag="PK264_10055"
     CDS             complement(9227..10609)
                     /locus_tag="PK264_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD76269.1"
                     /translation="MPDSVEHWPLDRLRAYARNPRTHSDAQVARIAASIVEFGWTNPV
                     LVAGDGTVIAGHGRLDAARRLGLSMVPVLVLDHLSEAQRRAYVIADNKLALNAGWNDE
                     LLAAELHVLNGDGFNLELTGFDEAELERLMAPLDDDSGAGGDATDDADEAPEPPRNPV
                     SRSGDLWRMGEHRLLCGDSTDGAAIARLMESERAALLFTSPPYGNQRNYTTGGIGDWE
                     ALMRGVFGRLDEVMAQDGQVLVNLGMIHRDNEWQPYWNGWLDWMRASGWRRFGFYVWD
                     QGPGLPGDWNGRLAPAFELLFHFNRQMRKPNKIVPCKWAGHINDSHGGMRGKDGTVGE
                     WTHAGQGVQETRIPDNVLRITRHKARGIETEHPAVFPVALPEFVMNTYCDRNDIVFEP
                     FAGSGTTLVAGERAGRRVHAIELAPEYVDVALLRWRQLFPSSAVVLDGDGQSFEAVAA
                     ERGIEIDDAA"
     gene            11184..11825
                     /locus_tag="PK264_10060"
     CDS             11184..11825
                     /locus_tag="PK264_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HRD76270.1"
                     /translation="MVASGSQQPQKPSQAERAQALLAERGMLRLRELTEAGIGEETLA
                     RLVRQGRVIRSSRGLYQLSDTEIDAAHSLAEVAKLVPKGVICLISALQYHGLTTQLPS
                     QVWIAIDRTAWKPAISYPPIRIVRFGDRSMNTGVKTHTIDGVPVRITSVAKTVVDCFR
                     YRNKIGTDVAIEALRESLQKRRCSASELWKLARDLRILSVMRPYLESAANDGA"
     gene            11815..12732
                     /locus_tag="PK264_10065"
     CDS             11815..12732
                     /locus_tag="PK264_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRD76271.1"
                     /translation="MAREIKNVAASIRARLLNHARETKRDFQLVLTHYALERLLYRLS
                     VSPFKDRFVLKGALLFTIWLDDPFRPTRDLDLLGHGSPDEEEMAAVFRKILSLTIDDD
                     GLTFDIDGLRAAAIREDARYGGIRIETTATIERARVPIQVDIGFGDAITPGADEIDYP
                     TLLSAEGVRLRAYPRETVVAEKLEALVSLGFINSRMKDFYDLVMMARHFDFDGPSLVQ
                     AVRSTFERRRTEIPTTVPDGLSDAFTSEPAVTARWRAFTTRDRLAADVVDFSEVILKL
                     RDFLMPVLSAASGAGPNLDHWPAEGPWRT"
     gene            complement(12747..13187)
                     /locus_tag="PK264_10070"
     CDS             complement(12747..13187)
                     /locus_tag="PK264_10070"
                     /inference="COORDINATES: protein motif:HMM:NF040389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6362 family protein"
                     /protein_id="HRD76272.1"
                     /translation="MADWTREQVEERLIEAADVIKRLPEVRVQGYFSVWPKIVHEFAD
                     LVGQEPPRMKRPPPLPDAISRMEATLPWMRWLEADDARLVWARAEGTPWKPICWRFGI
                     SRATACRRWEYSLCLITWRLNGRRPPSTKLGREHLVERVRFVYG"
     gene            complement(13180..13380)
                     /locus_tag="PK264_10075"
     CDS             complement(13180..13380)
                     /locus_tag="PK264_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76273.1"
                     /translation="MRWLPKGYGGERQSAEEVKREGWSEQGLLVVSPTDPRLTWPERE
                     LVRQLGEKLYGGRRQPTEHRHG"
     gene            complement(13377..13634)
                     /locus_tag="PK264_10080"
     CDS             complement(13377..13634)
                     /locus_tag="PK264_10080"
                     /inference="COORDINATES: protein motif:HMM:NF040395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HRD76274.1"
                     /translation="MSGETMLRHAASVVAERREIYGDPAAAMALVARRWSITLGRPVT
                     SAEVVLCLIDLKLARLGHDPKHQDSILDIAGYAAVLQEVGR"
     gene            complement(13631..14170)
                     /locus_tag="PK264_10085"
     CDS             complement(13631..14170)
                     /locus_tag="PK264_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76275.1"
                     /translation="MISTIETGPAAAGAIAFRPHPAHAHRAILGLDLGTTTGWALRSH
                     DGLITSGTVSFRPSRYDGGGMRYLRFRSWLDQIAADGGGLAALYFEEVRRHVGTDAAH
                     LYGGFLATLSAWCESHAIAHQGVPVGTIKRHIAAKGNADKAAVMAAVRARGFSPSDDN
                     EADAIAILLWAIETEGGVR"
     gene            complement(14278..>14987)
                     /locus_tag="PK264_10090"
     CDS             complement(14278..>14987)
                     /locus_tag="PK264_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76276.1"
                     /translation="GSYETWSRIMGGVLEVAGIEGFLANLDEMLAAADGEGAIWRSFV
                     AAWWDRFGTAEVGTGDLYEVALACEPPLPLGSGGDRSQRTRLGKALARMRDRVFDIDG
                     RKMRVRTLGVSHQAKRWQLTIEGERGERFPQGVEAQAGERCGQEGNVENQRSPAQPLE
                     TKGSGERGEHGERFSILTHARGCTRAIEDREQRSPCSPPSQSVGSSNAYDGEPGGERP
                     PSRSPDGAAPDWLKEVL"
BASE COUNT         2682 a         4964 c         4769 g         2572 t
ORIGIN      
        1 gacggcgcgg atcgccacgc cggatccggc gcctgccacc cgccagatcg catcgcgcgc
       61 gatgttcggg tcggcaaaca gcgtatccag cgcggcggaa agggcgctcg acatcagaaa
      121 ctggcgttga gacgcacccg gccgacgatg tcagccgcgc cgttggccac ggcttcgaca
      181 gcaacgccga tcagcgtgtt tgccgtcgcc gtcttggtgg tctctttggc ggtgttgtcc
      241 caatagatct tgtcgcctgc ggcccaggcc tgcgaaccga ccttcttgag gtcgaagacg
      301 ccgacgagtg ctgcttcgac cggctcggca ttggcggcgg ctgcggtggc gacaccgaag
      361 atggcgccga ccaagaggcc gtctcccgac gcaactgcgt agggggccgc cagggtgatc
      421 gtattgccag gttggacgta gttcttcatg gagaaactcc tctctgaatg acgaagggcg
      481 gctggaagcc gccctatctc gtcacgaagg ctgggggatg tgtgctttac gcgccggggt
      541 tcttgtaaag gccgcgccaa tcgatcgctt tcgcgccgaa atcgaggcgg cacttgatct
      601 cgacgccatc gacatcgaag ccgttgcggg tctcgatata ggcgccctgc tgaccttcga
      661 gataggcgta ctcgatggtg tcgatctgag ctggattggc ggcgagatac caggcgcccg
      721 tgctcaccgc atcgagacgc ggctcggaga tcggcgtcag cgtgcggatc gattgcggca
      781 ccacatcccc ggtcttcgca ggcacgaggt tctgggcgat gagctgctcg gccgccagtt
      841 cgagtgcggc cggcacgacc agatagctcg ggcggatgtt cagaaccgtc ttcttgtcga
      901 ggccggtttg cttggccatc gaggcgcgcc cttcgccgac cgccgcgacg ctgagtgcgg
      961 cgccggtacc ggcgaggttc ttgtgcgtcg cgtggaacag cgccacgcca tccgccatcg
     1021 cggcattggc cgtgacgatg ccccagacca cgtcgctttc gagcgtggcg atcgccgtgc
     1081 cgtacatcgc cggaatgcga gtgaaggcat cgagatcatc gttgatcagg acctgacgcg
     1141 tgatggcgac gacgcggcca taggtctcga tgcgatagct ttccttcgac tccgagatcg
     1201 tgccgcgctt gaactcgccg ccctcgctca ccttcagcag ctgcggcgct tcgccgagct
     1261 ggacccggtt catcgccttg aagtcggtcg cgagcacctg acggcagaac ggcacgaagg
     1321 tgcgcggata gacgtcatag gcctggcgca gcgtcttgtt ggtgacggcg gctagcacct
     1381 ccgggaagtc cgaggtcgag tgcagagcgc gcgtggcgat ctcgtcgcgc gacatgccgc
     1441 gcacattgac gccagccgaa gcgagaaact cccgcgacag ctccaggagc gtcatgccgc
     1501 gatactcgcg cgcgggatcg cttaagccga acagcgtcgg gctgtagcga tgcagcagcg
     1561 cattggcgac cgcctcgcga cgggtcagcc gttcgtcacg cccgccgagc ggaaccgaga
     1621 catgcgggaa ggtccgcgtc ttctcggcgg tgtcggccac cttgtcgaga atgacccggc
     1681 gggcctcgtc gatggcgacg ccccgcgtga caagatcttc ggccaggctg cgctccagac
     1741 cgaggcggct tgcgagatcg tagatggtgc cgacccgctc gcgctcggcc gtctgggcat
     1801 tggcgatcag ggcctgcgta tcgggctggg gatcggccgc acgggtgatc ggctcgggcg
     1861 tagcctgcgc cggcgtgggg gtcacattgg tctcttccat ggaagtcctc atctgttggg
     1921 aagcgtcgtc ccggtccacg acgcagggga tcagcgggtc aaccgaacgg aagccggccg
     1981 ccggatcggc cccgaccggg accgcggaaa tctcgaaagg tgtccagtcg accgcgcgcc
     2041 agacctccgg cgcattggcg ggtcgactga cctcgaagcg atggacctga tagccaatgg
     2101 agacggcccg cagatggccc gcccgcacat cggcccagat cggctcgaca tcgtcgcgct
     2161 cgctgaagcg gacccgggca atgccccgac cgctatcgat cctggcagtc cccggcacga
     2221 cggagccgat gacgctgtcg agtgtgcgga gatcatggac cttgagtagc ggccccccgg
     2281 cattgagccg ttctagccgc acgctgccgg gatccatgct gagttcttcg tcgaacggct
     2341 cgccgaatag cggctgccgc cggacccttg cgcccgtcga ccagaccacc tcgatcgagc
     2401 gatcctgctc gtcgagcgtg gccggcaaca ggtcggctgc gcggcgcatc gccggcagtt
     2461 cgatcgtgcc atgcatggtg tcgtccttac gtagaggtga gccctggatc gggctgcatc
     2521 acgccggtct tggtgacccg gcgtggatcg ctgtcgaaaa tgagtccgag cgcatcgatt
     2581 ttggcgttca tcgccgcgat ctcggccagc accgcatcgg gattgtgacc ctgccgggcg
     2641 atcgcttgtg ccaacgtcat ggtgccggac cgcatcgcca gaaggtcggc catggcatcc
     2701 ttcaacggat cgaccgcctc gaagcgcggc ggcgaccatt cgaccgggat gcgcggctgc
     2761 gggagcttgc ccgccgccca ggcctgttcg gtgaaccagt cccaagtggg ctggcagagc
     2821 atcggaatga cgatctgcca ctggaccgca tcgatcaggc ggcggaactc gacgagcccg
     2881 gcgcggatcg acgaatagtt gacctgactg agatcgccgg tcagaagctc gtaaggcatt
     2941 cggaaacccg ccgccacgat gtgcaactgg gcgcgcagcc attccgccac gccggccgtc
     3001 gttgctggct gattgaagcg gatatccttg ccgccgcgcg cataggcgat gaggccgggc
     3061 tcgaactgtt cgacccggtt gccatcggcg tccaccacgg acggtgctac tccctgctcg
     3121 gcctcatcgg cgccaagcac gatgccgacc acgcaggctt cggtcttctt gcgaaccagc
     3181 tcggcttgcg tccagtcgtc gagatcgcgc agtgcgcgca tcaccggcgt gccccacggc
     3241 acgccgcgta cctgcatgcg ctgcttctca tatagatgca cgatgtcggc agccgggatc
     3301 gcgagactgt cgagacgccg ccgcgtcgtg accacgctgt cgcccgggtg ctgcgcgaac
     3361 agccagaagg cacgccgctg tccgaggctt gagaactcga tcccctggac gacacgaccg
     3421 ccatccgcga gatcgccatt gcgcccggca tcgagcatgt cggcttcgag caactgcagc
     3481 tgcagcggca catcgagccc gtcactcgca cgacgtggcc tgcgccggat cagcacttcg
     3541 cctgcttcga tcatctggcg acaggccaag gtctgcagac ccaggaagtc gagttggccg
     3601 tcggcatcgc aaagcgctga ccatgcctcc cagagcgcat tcacttgcga gtcgagtttg
     3661 tcgtttccgc tcgccgcacg cgcgatgatg cccgagccga tgatgttatt gaccagaacc
     3721 gacaccgcct tggcggcatg cggattgttg cgcacgagat cgcgcatacg gtcgcgcagc
     3781 agcccgcccg cgacagcgat ctcgctgtcg gccgatgttc cggccacgcg ccagccatcg
     3841 gttcgccgcc ccttggcagc accgtcatag ccgcgcgcca aggcatcgaa gctctcgcgc
     3901 gcctgcacac ggcgcagtgc cgcccggggg gcgaccgagc cgatcgcgcg gtcaagccag
     3961 gagacgcggc gcatcagcgc tccccgcgcg agaacccggc gaaaccagcg acgggtggac
     4021 ggttttcggc agccccggcg atctcacgct cgatcgtgcg gatccgcgac aagaggtcgg
     4081 cggcggagcc gtattcgacg gtcttgccgt cgtagctgac ccgcaacgtg ccagacgcgt
     4141 aggcgcgccg cagcgcatcg agttcggctg tcgtccagct catcgcaacc atcctttgtc
     4201 cacgcccgag agccagtcgg agcgacgttt gccgccggca acgggagcac gcgccagcac
     4261 accggcggcg atcgacgcgt ccgctttcac atcgacgatt ccttcaggca caggtccgac
     4321 ctgatcttcc agatcgcgcc atttctcatc ggtccagcga tccgcgcccg cgatccaggc
     4381 agccgcccgg gcgtagaccc ggcagtcgag gacctcgttg cgttcgcgaa gcttctgcca
     4441 ttcgagccgc tggaacccgc gacgggtctt cgcgctcacg agttgctcgg cgacgagctg
     4501 cttgacccat tccgcttcga gcccgcgcgg cagatgcacg aagcccgctg gatattgcgc
     4561 gccctcggcg atttcctcgt cggtcgggcg cgtgagacgc agatgccgat aggtttcgct
     4621 tttgaacgtc gccaccgcga ccgtccaaag cctggcaccg cgccgcagct tgcggccggc
     4681 ttccgtgaca tcgacgaaac ttgggcccac gacgggtgcg gcccgattga agccttcgac
     4741 acccttcagg ggtacgacct gcgcatggcc cgccgatcgt gcccaggcat agacggctgg
     4801 cgactcgtag ccgctatcga tgccaagttt cgcgagacca agccgcgcgc catgggcatg
     4861 cggccacgtc cggtcgagca ggcctgagag ttggctccaa gtctcggcat gctcgggccc
     4921 gccatcgatg acgatatggt cgacgaacca gctggtcagt ccccgccccc aggcccagac
     4981 cgagacctcg atccggtcct tctggacgtc ggcgccagcg gtcaggaaca aaccaccgct
     5041 cggcacggtg ccgatctgcc agtcctcgcg gcgctcatag agtcgttgcc agtcgggcgc
     5101 ttcgccggtt tcgatccagg tctcgcccaa cacgccgttc ttgaaactgc gcttggcttc
     5161 attggtcgcc agcgacgctt cccacatgcg cgcgatcatc gcccagctca tccagccgac
     5221 cggcgagtaa agcgccgaca gatggaaccc gatcgtgccg ggatcggcgg cttccgccgt
     5281 cggacgccag tcgccggcat ggagcatttc gatcttgtgg tgctcttcga tctcgccgtc
     5341 gcaggcttcg cattggtaat gcgcggaatc gggctttccc ttctcccagc gcagccgctc
     5401 gaagcgcagc cactgccgat gctggcaatg ggggcaaggc acgaagaatc gccgctgatc
     5461 ggacgcatcg aactcgcgct cgatccgcga cacgccatag atcgtcggcg tcgaagcgag
     5521 aagcactttc gaacgccacg agaaggtgcg cgtgcgcgcc tcggcgagcg cgaccggatc
     5581 gccttcctcg tcggccgagg gcggataggc gtcgacctcg tcgagaaaga gatagcgcgc
     5641 cggcatcgag cgcaggccga ccgcactgtt cgcaccggtg atgaccagaa gaccggccgg
     5701 aaactccttc gacaggaccg tgttgccggc atcccgcgcg cgcgccggtt tgacccgctc
     5761 gcgcaacgcc gggctttcgg cgatcaaggg ctcgatgcgc tggcgcgaaa agcgcttggc
     5821 aagctcgacg gtcggctgca ccgagagcat cggacccggc gcatgatgga tgacgtagcc
     5881 gatccagttg ttgccggcct cggtcgcccc gacctgtgct gccttcatga acacgatgcg
     5941 ccgcgcgggg tgcgacggcg acagcgcatc catgatcgcg cgcatatagg gcgtgcgatc
     6001 ggttcgatag cgccccggtt cggccgaggc gcgcgggctc aggaagcgat gccggtccgc
     6061 ccattcggaa acagtgagca gcggatccgg ggtgagaccg tctcgccagg attgccatag
     6121 ctcttcagca ccctcgaagc cgaacagatc aacggaactc tggtcggacc tcggcaagtt
     6181 cggcgaggtg ggcgcggaca tgtgtctcta aagccttctg catcggatgc gcctccacgc
     6241 caagctcggc cgcgatcagc gcagcgacac gggcgggcca attgacccag gtgtcccgct
     6301 cctcgcgcgc catgcggaag acgagtgcgg ttgcgcgtgc ccggtcgatc agctcgcctt
     6361 tcatgcgctg gagccgcagc cgcgcgagat gcgccttggc gatctcatgg gcggtccgcg
     6421 cctggacgaa ggtcacgttg ccgctcgccg gcaggccctg ctccttgagg gtctcccgaa
     6481 ccgaccccat ggcggcctcg gctacgggct tgagcttttc gggcttggcc ctggaccgcc
     6541 cgggttcggt cgaacgctcc caggaaatgt cggccttggc cgggtcgatc gtgccatccg
     6601 gctcaaggga tacgcgcccc gccttggcag cgcgcagcac cgcgacgtgg ctcacgccgc
     6661 gatgccgggc ataggcgcgg attgataatc ccatgatcgt cctggctcga agaaagcaat
     6721 caaatgctgc gcttaagagc ttggctccgg gccaaagcag cgcgtgtatg gcgtcaccat
     6781 caaggaacgg agagcgccat gaccaagacc ccgcttaagc aaaccgccct cgacgccttc
     6841 atcgctcgca aggccgaaat cgacgcgatg ctcgaccggc tgaagagcct gagcgacgac
     6901 catttcgact acgcacccga cgacatcgat tggggccatg tcgggacgct cgcccattac
     6961 gccgagcttc tgaagcgcat caccgacagc gcgttcaagg aaggcgaaca cgccgaatag
     7021 gcgaccctca tcccgcttat gccccgaccg gccaggccgg cggggcttgg gctcgtagga
     7081 gggccgcgat ggtcgcggct cgctaggcaa ggagccaaca ccatggcgaa actttccgat
     7141 tcccaactcg tcgttctttc gaccgcctgc caacgccctg atggctgcgt cttcccggtc
     7201 accctgaaac tcaagggcaa tgccgtcggc aacgtcctga agagcctcct caccaaggga
     7261 tttctcgaag aagtccctgg ccgcgcagac gataccgtct ggcgctacga cgatggcgcg
     7321 ccgctgacgc tacaagcgtc gcgcgccgcc tatgacgaac ttggtatcga gcccgagacg
     7381 gatcccgcca ccgagggacg ccatgctgcc gccatcgacg attgcggact ggtgagcggc
     7441 agcccagccg agcaaggcat cgtcgcggcc aagatcgaaa cccgcgcgcg caagaccgat
     7501 caggcgccgc gcacgcgcgg agacagcaag caggcgaagc tcatcgagat gctcaagcgc
     7561 cccgagggcg ccagcatcga ggagatcgtc gcggcattcg actggcaagc ccacacggtc
     7621 cggggtgcga ttgccggcgc cctgaagaaa aagctcggtc tcgacgtgac gtcggagaag
     7681 atcgacggac gcggccgggt ctatcggatc gcctgatcgc gccaaccatc agcgccgtcg
     7741 ggcatcaaag cctgacggcg cttttgcgtt cggcgcgcac ccggaattcc tcgaacacgc
     7801 ggcgcagcgc aaaggatcgg gcaatcgata cgaacgtgaa gaccaagccg atgaccagat
     7861 tgtcggcgag cgaaacctgc agaccgaaga tcgggaagac cgcgatctgc gttagaacgg
     7921 cgatgccgaa gccgaccacg acattagcga tcgattcgac cagcgacatc cttcgggatt
     7981 gcttgttcat gcgccgcgct cggatcgtac atcttcgacg gaccgaccat cggtctcgag
     8041 ttgcgccttg gagccggtga aggattgcca gcgctcgatg atcacatcgc agtatcgcgg
     8101 atcgatctcc atcgcgaagc agcggcgctc gatgctctcc gccgcgatga gcgtcgtgcc
     8161 gctaccggca aaaggctcat agacggcatc gccgcgctca ctgttattga ccatggggcg
     8221 acgcatgcat tcgaccggct tctgcgtgcc gtggacggtc gccgcatcct catcaccacc
     8281 gccgccgatc gtccagaccg tcgattgatc gcgcgcgccc tgccaatggc cggtcgcgcc
     8341 cttccgcacc gcgtagaaac agggctcgtg ctgccagtga tagtcgcccc ggccgagcac
     8401 gaagcgcgac ttcgaccaga tgatctgcga gcggatcgga aacccgctgg catcgagact
     8461 ttcggccacc gtccgggcgt gaacacccgc gtgccagaca taggcgacat cgccggggaa
     8521 gagcgcccaa gcctcgcgcc agtccgcacg atcgtcgttg gatacccggc ccgtgcgggc
     8581 ggtggccgat acaccagcat cgttgcgcca ggacgggtcg tagtcgacgc cgtagggcgg
     8641 atcggtgacc atcagatgcg ggcgaacatc gccaacgagt cgcgcgacat ccgaagcaca
     8701 agtcgcgtcg ccgcaaagga ggcgatgacg cccgaggatc cagagatcgc cgggccgtga
     8761 gatcggcgtc tccggtggct ccgggacgac atcctcgtcc tcgttgccgc ccttctcgcc
     8821 gtccagccca gcgagcagcc gatcgagctc atcctcggga aaaccgagaa gctccagatc
     8881 gaccccatct tccttgagcc gcgcaacctc ggccgacaac agcgcatcgt cccatccgga
     8941 attgagcgcg atctgattgt cggcgatgcg ataggcacgc gcctgcgcgt cggtcagatg
     9001 gtcgagccga atgaccggga cctgtgaaag tccgagacgc tgggcggcga gcaggcggcc
     9061 atgaccggca accagcaccc cgcgctcgtc gacgaggcac gggacgttga agccgaattc
     9121 cgcgatcgat ccagcgatct gggcaacctg ctcatccgga tgcgttcggg cgttggccgc
     9181 atagggcaat agccgatcca aaggccactg ctcgatttgg atatggtcac gctgcatcgt
     9241 cgatctcgat gccccgttcg gccgcgaccg cttcgaacga ttgcccatcg ccatcgagca
     9301 cgactgcgga agacggaaaa agctgacgcc aacgcagcaa ggcgacgtcg acatattcgg
     9361 gcgccaactc gatcgcgtgc acgcgacgac ccgcgcgctc gcccgccacc agcgtcgtgc
     9421 cggatcccgc aaatggctcg aagacgatgt cgttccggtc gcagtaggtg ttcatcacga
     9481 actcaggcag cgctaccggg aataccgccg ggtgctcggt ctcgatgccg cgcgccttgt
     9541 gccgggtgat gcgcaggaca ttgtcgggga tgcgggtttc ctgaacgccc tggccggcat
     9601 gggtccactc gccgaccgtt ccgtccttgc cgcgcatgcc gccatggctg tcgttgatgt
     9661 ggccggccca tttgcagggc acgatcttgt tcggcttgcg catttgccga ttgaagtgaa
     9721 agagcagctc gaatgccggc gcgagccgac cattccagtc gccggggagc ccggggccct
     9781 gatcccaaac gtagaagccg aagcgtcgcc atccggacgc gcgcatccag tcgagccagc
     9841 cattccaata gggctgccac tcgttgtcgc gatggatcat gccgagattg accagaacct
     9901 ggccgtcttg cgccatgacc tcgtcgagcc gaccgaacac gccgcgcatc aaggcttccc
     9961 aatcgccgat cccgccggtc gtgtagttgc gctgattgcc atagggcggg ctggtgaaga
    10021 gcagtgcggc gcgctcgctt tccatgagac gcgcgatcgc agcaccgtcc gtgctgtcgc
    10081 cgcaaagcag gcgatgctcg cccatgcgcc agagatcgcc cgatcggctg accggattgc
    10141 ggggcggctc gggcgcctcg tcggcatcgt cggtagcatc gccgccagca ccagaatcgt
    10201 cgtcgagcgg cgccatcagg cgctcgagtt cggcttcgtc gaagccggtg agttcgagat
    10261 tgaagccgtc gccgttcagc acatggagct cggcggcgag caattcatcg ttccagcccg
    10321 cgttcagtgc cagcttgttg tcggcgatca cataggcgcg gcgctgcgcc tcgcttagat
    10381 gatcgagcac aagcacgggg accatgctca atccgagacg ccgcgcggca tccaggcggc
    10441 catggccggc aatcaccgtc ccgtcgccag cgaccaggac cgggttggtc cagccgaatt
    10501 cgacgatgct ggccgcgatc cgggcgacct gcgcgtcgga atgcgtgcgc ggattgcgcg
    10561 cataggcacg caggcggtcg agcggccaat gctcgacgct gtcgggcaag cgaggttgca
    10621 tgacgggatg gcgttaccag gggttcgatg ttcggggtgg taaccggctt tgccggttac
    10681 cggatcgagt taccagtccc gcttgcccgc aaggtcgcgc catatcaaag gcttgaagcg
    10741 gctgatcgcg ccggggccgt ggtaactggt aactcggatt tcggggctgt cggaagcgaa
    10801 cttccgcgcc actgccgcca gcatacggtt tcggccagga aggacccgcg atatcagtgg
    10861 cttaggcgat ttgggtgtaa atgggcgtcg aattgggtat ttgcgcccta aagaaaggat
    10921 atcggcgtgt cttttaatta gagcaggggc caaataaagg atgcgaaccc tagggtccgc
    10981 attgcatcgg tccatgcgct ccttgcccga gcatggtcag aatctagcgg aaatccggcc
    11041 gttccgtctc gtctgggggt gtctcacgga aagatgtctc acgttccgct ttcgggttga
    11101 cagccggtag cactccgctt ggctccgtct attatatgtg ataaaacctc tgcactaagc
    11161 tgccttagcc aaggaatcat cacatggtcg caagcgggtc gcagcaacct cagaaaccct
    11221 ctcaagccga gcgtgcccag gcactgctcg ctgagcgtgg catgctgcgc ctccgcgaac
    11281 tcaccgaggc cggaatcggg gaagaaactc ttgcccggct tgttcgccaa ggccgagtca
    11341 tccgatcatc ccgcgggcta taccaactat ccgataccga gatcgatgca gcgcatagcc
    11401 tcgccgaggt ggcgaagctc gtgccaaagg gtgtgatctg cctgatctcg gctctccaat
    11461 atcatgggct cacgacgcag ctaccatcgc aagtctggat agccatcgac cgcacggcat
    11521 ggaagccagc aatatcgtac ccgccgatcc gaatcgtacg atttggcgac cggtcgatga
    11581 acacgggcgt caagacacat acgatcgacg gcgttcccgt ccgcatcacc agcgtcgcca
    11641 agaccgtggt tgactgcttc cgctaccgca acaagatcgg gacggatgtc gcgatcgagg
    11701 ctcttcgtga aagtcttcaa aaacgacgct gctccgctag cgagctgtgg aagctcgcgc
    11761 gcgacctccg aatcctgagc gtcatgcggc cctatctcga atcggcggcg aacgatggcg
    11821 cgtgaaatca aaaacgtcgc cgcttccatc cgcgcccggc tgctcaatca cgcgcgggaa
    11881 acaaagcggg atttccagct ggtgctcaca cattatgccc tggaaaggct gctctatcgc
    11941 ctcagcgtat cgcctttcaa ggaccgtttc gtactcaaag gagcattgct cttcacgatc
    12001 tggctggatg atcctttccg ccccactcgc gatctcgacc tgctcgggca cggtagtccc
    12061 gatgaggaag agatggctgc ggtcttccga aagattctat ctctgaccat cgatgatgat
    12121 ggcctcacct ttgacatcga cggcctccgc gccgcagcca tccgcgagga cgcccgctat
    12181 gggggcatcc gcatcgaaac caccgcgacg atcgagcggg ctcgggtgcc cattcaggtc
    12241 gatatcggct tcggcgacgc gatcacgccg ggtgcagacg aaatcgacta tccgacgctg
    12301 ctcagcgcgg agggggttcg cttgcgcgcc tatccgcgcg agacggtggt cgccgaaaag
    12361 ctcgaagctc ttgtatccct cggcttcatc aatagccgca tgaaggattt ctacgacctt
    12421 gtcatgatgg cgcggcactt cgattttgac gggccaagcc tggttcaggc ggttcgatcc
    12481 acatttgaac gacgccggac agaaattccc acaacagtcc ccgatgggct cagcgacgcc
    12541 tttacgtcag agcctgcggt taccgcgaga tggcgcgcat tcacaacgcg cgaccgcctg
    12601 gccgcagatg tcgttgactt cagcgaggtc atcttgaagc tccgcgactt cctcatgccc
    12661 gtgctgtccg ccgcgagcgg cgctggcccg aatctggatc attggccggc cgaggggcct
    12721 tggcggacat agcactgact caccgatcat ccgtaaacga atctcacccg ttcgacgaga
    12781 tgctcgcgtc ccagttttgt cgaaggcggg cgccgtccat tcagccgcca cgtgatcagg
    12841 caaaggctgt attcccagcg gcggcacgcc gtggcgcggg agatcccgaa gcgccagcag
    12901 atcggcttcc acggcgtgcc ctcggcgcgc gcccagacaa gccgtgcatc gtcggcttcc
    12961 agccatcgca tccagggcag cgtcgcctcc atgcggctga tggcgtccgg caagggcggc
    13021 ggacgcttca tgcgcggcgg ctcctgaccg acgagatcgg cgaactcatg cacgatcttc
    13081 ggccataccg agaaatagcc ctgcacccga acctcgggca ggcgcttaat gacgtcggca
    13141 gcttcgatca accgctcctc gacctgttca cgagtccaat cagccatgcc gatgctccgt
    13201 cggctggcgc cgtccgccat agagcttctc gcccagctgg cgaacgagtt ctcgctcggg
    13261 ccaggtgagg cgcggatcgg tggggctgac gacgaggagg ccctgctcgc tccagccctc
    13321 ccgcttgacc tcttcggccg actggcgttc gccgccatat cctttcggca gccatctcat
    13381 cgtccgacct cctgcagcac cgctgcatag ccggcgatgt cgaggatcga atcctgatgc
    13441 ttcggatcgt gcccgagccg cgccagcttg agatcgatga ggcagagcac gacctccgcc
    13501 gacgtgacgg ggcggccaag tgtgatcgac cagcgtctgg cgaccagggc catcgcggcg
    13561 gccggatcgc catatatctc gcggcgctcg gcgacgaccg acgcggcgtg cctgagcatc
    13621 gtctccccgc tcatcgcaca cctccctcgg tctcgatggc ccagagcagg atggcgatgg
    13681 cgtcggcttc attatcgtcc gagggcgaaa agccgcgggc gcgaacggca gccatgactg
    13741 cggccttgtc ggcgttgccc ttggcggcga tgtgccgctt gatggtgccg acgggaacgc
    13801 cctgatgggc gatcgcgtgg ctctcgcacc aggcgctcag cgtcgccagg aaaccgccat
    13861 agagatgggc ggcatcggtg ccgacatggc ggcgaacctc ctcgaaataa agcgccgcga
    13921 gaccgccgcc atcggcggcg atctggtcca gccagcttcg gaaacgcagg tagcgcatgc
    13981 cgcccccgtc atagcggctc ggccggaacg agaccgtgcc gcttgtgatc aggccgtcat
    14041 ggctgcgcag tgcccagccg gtcgtggtgc cgagatcgag gccgagaatg gcgcgatgcg
    14101 catgagccgg atgcggacgg aatgcgatgg cccctgctgc ggcggggccg gtttcgatgg
    14161 tcgagatcat gattgtctcc aaggagcgcg agcaagggtc gggtttcgga tcgaagaccc
    14221 atcgcggcgg accggtcgct gccgccagga gacggtcgag atgacggagc gcgctcatca
    14281 gagcacctcc ttcagccaat cgggcgcagc gccgtccggg gaacgtgagg gaggacgttc
    14341 cccgccaggt tccccatcat aagcgtttga agaaccgacg ctttgggaag gtggggaaca
    14401 tggggaacgt tgttcccgat cctctatcgc gcgggtgcag ccgcgcgcat gcgttagtat
    14461 tgagaaacgt tccccatgtt ccccacgttc cccagagcct tttgtttcaa ggggttgggc
    14521 cggggaacgt tggttttcga cgttcccctc ttggccacaa cgttccccgg cttgagcctc
    14581 gacaccctgc ggaaaacgtt ccccacgttc cccttcgatc gtgagctgcc agcgcttggc
    14641 ctgatgggag acgcccagcg tgcgcacgcg catcttgcgg ccgtcgatat cgaacacccg
    14701 gtcccgcatg cgggcgagcg ccttgccgag ccgtgtgcgc tgtgaccggt cgcccccgga
    14761 gcccagcggc agcgggggct cgcaggccaa cgccacctca tagaggtcgc cggtgccgac
    14821 ctccgccgtc ccgaagcggt cccaccaggc ggcgacgaag ctgcgccaga tcgcgccctc
    14881 accatcggcg gcggccagca tctcgtcgag attggcgaga aacccctcaa tcccggcgac
    14941 ctcgagcacg ccgcccatga tgcgcgacca ggtctcgtaa ctcccga
//
LOCUS       DAORNH010000080        14922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3038_length_14922_cov_2.463106, whole genome shotgun sequence.
ACCESSION   DAORNH010000080 DAORNH010000000
VERSION     DAORNH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14922
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3038_length_14922_cov_2.463106"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1698)
                     /locus_tag="PK264_10095"
     CDS             complement(<1..>1698)
                     /locus_tag="PK264_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76277.1"
                     /translation="AMLIATNSGIYRSTNYRDQNAEAVTFTRAGQPHAQVHMPQVECS
                     RSVAQHCVAGSILGTAMVSRDAGSTWTPATGFPAVPPNARSGMRIELAMSAANPTIVY
                     ASVDRNLGELYRSDDGGQTFTLRSTGANWLGSIRNGVPNSQGWYANAIWAGDPTRPDL
                     VVVGGLDIFRSLDGGRTFTWIADWTISPRSPHADHHVLAAHPGYNGTTNRTIYNGNDG
                     GIYRHDNIIVAEPTRGWILLNTNYGVTQFYSVAGDPKTGRIVGGTQDNGTLAYDPPAS
                     GPPADRFRRIYGGDGSYAAVDPTDPNFVYGATQFLGLVRSRDGGFATERISEGVFDSG
                     AASGSNFIAPFILDPNNPNVLFAGGESLWRTREAKGPRPAWQQVKMAGEGSGNHVSAI
                     EAARSGKAAGPGSDIVWVGHNMGDLYVSRNASAREPEWTKLDVKGLKLPARAVTRIRV
                     SSHNPAIVYVSYAGYEKTNLWRTRDDGKSWENVHGALPEISVRDVAVHPVNPRWLYAA
                     THVGLFASADEGVTWTASNRGPANVAVHQLIWMGERLVAATHGRGIYEYSFATQQFVY
                     "
     assembly_gap    1699..1785
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1786..2629)
                     /locus_tag="PK264_10100"
     CDS             complement(<1786..2629)
                     /locus_tag="PK264_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76278.1"
                     /translation="MQSRSLIVTIALSTAVAISMAAAQAPTEVDVAKRLMISVEEVRL
                     IREKRGIRLEQLALMTRDRAIKILGRIQAPLPENPLGAGEYMALMETGRKDGKRRVDA
                     IERAVSQTAIGRARALSAVTRPVGAEVRTTAAGQVIEIAGIPVADTGPRVAATGDARA
                     AGMDTLSFEPILPLTAMRNREPGDLSPRRWNWIGPTNIGGRTRAIVINPQDPNVMYAA
                     GVSGGVWKSINAGQSWAPLADFMASINVTTLALDRSNPNIIYAGTGEFWGRSEFGPGL
                     GVFRS"
     gene            complement(2818..3504)
                     /locus_tag="PK264_10105"
     CDS             complement(2818..3504)
                     /locus_tag="PK264_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD76279.1"
                     /translation="MPNYVIAPPAQAALPIRGTDKLFPVHRIYCIGRNYAEHAREMGH
                     DPNKEPPFFFLKNPDNIRLDGRFPYPAGTKDVHFEIEMLVALKSGGTNIKAEDALNHV
                     WGYGVGLDMTRRDLQSEAKKLGRPWEVGKAFEHSAPATPLVPASEIGHPSKGAIWLEV
                     NGQKRQTADLSMMIWSVPQQIAILSSLFEMRAGDIIMSGTPAGVGATVKGDKLKGYVE
                     GVDTLEVTVV"
     gene            3666..4931
                     /locus_tag="PK264_10110"
     CDS             3666..4931
                     /locus_tag="PK264_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRD76280.1"
                     /translation="MARAAAKKSKCLDHQERLDRLAHIAVRVGLNLKRGQDVIVTAPV
                     EGVDLARLVVKHAYEAGAGLVTVLYDDEEATLLRYKHAANASFDKAPGWLHDGVAKAY
                     EQGAGRLAIRGGNPSLLAGQDPAKVSRVNRAQSIAAKPMMERITGFDINWTIVPYASA
                     AWARAVFPEASETTAVRKLWNALFMTTRVDADDPVAAWSEHNKQLARRSKWLNQRSFA
                     SLHFKGPGTDLTVGLGDGHVWCGGAERAKNGIVCNPNMPTEEVFTAPHKDRVEGTVTA
                     TKPLSYMGTLIEEIAVRFEKGAIVDAKAKKGGDVLHKVLETDDGARRLGEVALVPYTS
                     PISKSGLLFYNTLFDENAASHIALGQAYTSTFKDGAAESPARLAGRGANRSLIHIDWM
                     IGSNKVDVDGIAADGSAEPVMRGGEWVSA"
     gene            4948..6118
                     /locus_tag="PK264_10115"
                     /pseudo
     CDS             4948..6118
                     /locus_tag="PK264_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            6115..7014
                     /locus_tag="PK264_10120"
     CDS             6115..7014
                     /locus_tag="PK264_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD76281.1"
                     /translation="MIGRIGRAIYDNAYFLLAATSLFWAGNQIVGRMVAGHVPPVTLG
                     FSRWLLASLIITPLAWPHLTRDWSIVRANLPLLAFLGVIGGGTLNTLQYIGLNYTTAL
                     NALVLNSAAPVLIAVACTLIFRDRLTRLQSLGIAISLAGVLAIIARGDPAGLLALSFN
                     RGDAIIFVGMVSWAVYTAFLRKRPALHPFAFVAVMFWAAALVNLPFAALELAGGQRLH
                     PDLKTTAAILYVGLFAGVLAYLAYVRGVELIGGPRAGAFTHMIPFFGAILAMLFLNEA
                     LASYHLYGLALILFGVWLAARPK"
     gene            7104..8126
                     /locus_tag="PK264_10125"
     CDS             7104..8126
                     /locus_tag="PK264_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HRD76282.1"
                     /translation="MTADDRAFDLGRADFVSTAPPAAGQIDLRLIAAVAAVRDDEPMI
                     LTVRGADGDQLPAGGFTPSMDRTLEAGVRSTVSRDAGLDLGHVEQLVTLSDHRASPYA
                     PPASSHRISVAYLALARMGESSEGHGASRSWKSWYTYLPWEDWRQGRPTILDEQIIPR
                     LAAWAQEPDFGRSNATALGRGERVKIAFATGESAWDEERVLERYELLFESGLLEEARA
                     EGRRAPLGPKMSALGRPMQFDHRRILATAISRVRGKIKSRPIVFEMMAAEFTLFELQR
                     TVEALLGPHLHKQNFRRLIEAGRLVEPTGSVRSRTGGRPAKLFRFRSEVVVERAAPGV
                     RVRPPR"
     gene            complement(8140..>8431)
                     /locus_tag="PK264_10130"
     CDS             complement(8140..>8431)
                     /locus_tag="PK264_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRD76283.1"
                     /translation="PHLALIDGNRAPHLACPTRTIVKGDARCLSIAAASIIAKVTRDR
                     LMIALARELPGYGFERHKGYGTPEHQDALRRLGVTEQHRRSFKPVQLALEIA"
     assembly_gap    8432..8560
                     /estimated_length=129
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8561..8751)
                     /locus_tag="PK264_10135"
     CDS             complement(<8561..8751)
                     /locus_tag="PK264_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949752.1"
                     /note="cleaves the the RNA-DNA junction of a RNA-DNA/DNA
                     heteroduplex; does not have RNase H activity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRD76284.1"
                     /translation="MIKATFELEEAELRLGRGPIAGIDEAGRGPWAGPVVAAAVILDP
                     ARIPAGIADSKAIDADDREA"
     gene            complement(8822..10096)
                     /gene="purD"
                     /locus_tag="PK264_10140"
     CDS             complement(8822..10096)
                     /gene="purD"
                     /locus_tag="PK264_10140"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRD76285.1"
                     /translation="MNVLVIGSGGREHALAWAIVASPLATRVYVAPGNAGIAEVATCV
                     PLNVSKHDEIIRFCRETRIDFVVIGPEAPLVAGLADDLAAAGIKHFGPSKAAAALEGS
                     KGFTKDLCAEVGIPTGAYGRFSDKAAAMAYLARQPVPIVVKADGLAAGKGVVVATTRD
                     MAEAAVEACFAGAFGSAGAEVVIEEFLEGEEVSFFALCDGTSVLSLATAQDHKRVGDG
                     DTGPNTGGMGAYSPAPVVTPAIHKRIMAEIIRPTVAAMARRGTPFKGVLFAGIMITPA
                     GPKIYEYNCRFGDPECQVLMMRLKSDVLAALLATADGVLGTFDLRWHDEPALVVVMAA
                     EGYPGEPRRGTEIRGLAEATTVPDVEVFHAGTKRDGDRILANGGRVLGVAARGKTVAE
                     AREKAYAAVDRIQWPEGFCRRDIGWRAVGRST"
     gene            complement(10117..10998)
                     /locus_tag="PK264_10145"
     CDS             complement(10117..10998)
                     /locus_tag="PK264_10145"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRD76286.1"
                     /translation="MTKSVLGIIGGSGLYDLPAFENPRWQAVASPWGEPSDAILFAEV
                     AGLPIRFLPRHGRGHGTPPSAINYRANIDALKRAGVTDLISISACGSLKEEFPPGHFV
                     LVDQFIDRTFAREKSFFGTGCVAHVALADPVSPGLVDLIEKAANAEGIAATRGGTYIV
                     MEGPQFSTRAESNLYRSWGCSVIGMTNMPEAKLAREAELPYATVAMVTDYDCWHEDHD
                     DVDVAAVIKVLTANVAKAQRLVTRLAREFPREHPKCPVGSDHALDVAIITAPDKRDPA
                     LVAKLDAVAGRVLGKKA"
     gene            complement(11092..11880)
                     /locus_tag="PK264_10150"
     CDS             complement(11092..11880)
                     /locus_tag="PK264_10150"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HRD76287.1"
                     /translation="MRLKSFALAGLALAIGTTTAVLAQRGPGPGPAPFGAPKSHVAGQ
                     FDYYALVLSWSPTHCATATPGRDDMQCRPRNNKRYSFVLHGLWPQYERGFPESCQTRE
                     RPFVPDALITRMLDIMPARGLVIHEYRKHGTCSGLSPEGYFETSRKLFSKVKIPDRFD
                     EPAAAQFVSPAELIAAFVAANPGLKTESIAVQCSRPGDRLKEVRICFTRDGAYRACGN
                     NENQSRLCSAARMYVPPVRPGAPGAVPNPKAPNTPPPLPGDRKI"
     gene            12115..14130
                     /locus_tag="PK264_10155"
     CDS             12115..14130
                     /locus_tag="PK264_10155"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HRD76288.1"
                     /translation="MPVPSLIELDISERFAFADGHAFGDAGSYERLKGRARFAVDPTA
                     QAQAGIVDLDKAPRGADGRVTFTADISILKPSDLARSNRRLFFDWGNRGNIRCLQFFN
                     DAPGSNDPRTRAHAGNGFLLRRGYSLVWCAWQADLLAGDHRFLLDVPVATDNGRPVTG
                     PVRVEYIADQAGITTFPLSSRASTRSHPAVSLDTRKATLTRRRYPGDPRQPIPHDQWM
                     FARVEGGTGLDNQGAEFSVIPSDTHIHIPKGFETGWIYELVYEGRDPLVLGLGHVAVR
                     DLVSFLRHGETGNPLRAGSVAMEKAYGWGRSQTGRCIRDFIHRGFNADAAGRRVFDGL
                     LPHVSGAGLMWLSRFANVIVAAGQQYEDHYNPADQFPFTYAPTTNHNTGKSDAIMKRP
                     ATDPLVLHTQTATEYWQRRGSLVHTDTKGNDVALPENVRAYLWSSSQHFADPRLAKPA
                     RGVCQNVVNVVWTSMLFRAMLDALDAWATKGTPPPASRIPRRSDGTLVTLEEWRKAFP
                     AIPGVMTPRSPSEAPLLDWGPEASRGILIEPPQVLAEQGYAVLVPQVDKDGNDIAGVR
                     APMVEAPLATYTGWNLRTRGVGHGAMHEFTGSTLPFPGTAEEARATGDPRRSIAERYG
                     TQRGYAEAIVAAARRLVADRLMLEEDVERCAASAANWHAPRHDVGLP"
     gene            14130..>14922
                     /locus_tag="PK264_10160"
     CDS             14130..>14922
                     /locus_tag="PK264_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HRD76289.1"
                     /translation="MLNPPVIEGARDLLARYDAVFCDVWGVLHDGRDAFKSACSALIA
                     FKAQGGAVVLVSNAPQPKERVGEILDERNVPREAWDAIVSSGDLALEHVAKSGFRRTA
                     RIGPLPRSLPFFARLSGEAAPIETAEAIVCTGLVDDHNETAKAYRPMLAKALARRLPF
                     ICANPDLIVDVGGRLYLCAGAVAKLYEEMGGEVFWAGKPHLPAYHRARSEAERLLARS
                     IPKSSILAIGDAMRTDIAGAAGFGIDSLFVANGIHRHEVTDNGHID"
BASE COUNT         2404 a         4984 c         4853 g         2465 t
ORIGIN      
        1 atacacaaac tgttgcgtcg caaacgaata ctcatagatg ccgcgcccgt gcgtcgccgc
       61 gaccagccgc tcgcccatcc agatcaactg atgtacggcg acgttggccg gaccacggtt
      121 ggacgcggtc caggtcacgc cctcgtcagc gctggcgaac aggccgacgt gcgttgccgc
      181 gtaaagccag cgtgggttca cggggtgcac ggcaacatcg cggaccgaaa tttccggaag
      241 cgcgccgtgg acgttctccc aggacttgcc atcgtcgcgc gtgcgccaga ggttggtctt
      301 ctcgtaaccg gcgtagctca catagacaat cgcgggattg tgcgagctca cgcgaatgcg
      361 ggtgacggcg cgcgctggca gcttcaggcc cttcacatcg agctttgtcc actcgggctc
      421 gcgagcgctg gcattgcgtg agacgtagag gtcgcccatg ttgtgaccga cccacacgat
      481 atccgaaccg ggtccggctg ccttgccact gcgcgcagcc tcgatagcgc tgacatgatt
      541 gccggaaccc tctccggcca tcttcacctg ttgccaggcg ggacgcggcc ctttcgcctc
      601 gcgcgtgcgc cacaggctct caccgccggc gaacaggacg ttcggattgt tgggatcaag
      661 tatgaacggc gcaatgaagt tcgagccgga tgcggcaccc gaatcgaaca cgccctcgct
      721 gatccgctcg gtggcgaagc cgccatcgcg gctacggaca agacccagga actgcgtcgc
      781 cccgtagacg aaattggggt ccgtagggtc gacggcggca tagctaccgt cgccgccata
      841 gatgcgacgg aaccgatcgg ccggcggacc gctcgccggc gggtcgtagg cgagcgtgcc
      901 gttatcttgc gtaccgccga cgatgcgccc ggtcttcggg tcgccggcaa cgctgtagaa
      961 ctgcgtcacg ccatagttgg tgttgagcag tatccagccg cgcgtcggct ccgccacgat
     1021 gatgttgtcg tgccgataga tgccgccgtc gttgccattg tagattgtgc ggttggtcgt
     1081 accattgtag cccggatgcg ccgcaagcac atggtggtcg gcatgcggcg accgtggcga
     1141 gatggtccag tcggcgatcc aggtaaatgt tcgcccgcca tcgaggctgc ggaaaatatc
     1201 gagaccaccc acgacgacga gatcgggccg ggtcggatcg ccggcccaga tagcgttggc
     1261 ataccagccc tggctgttcg gcacgccatt tcggatcgag ccgagccagt tggcccccgt
     1321 gctgcgcagc gtgaacgtct ggccgccgtc gtcggaacga tagagctcac cgagattgcg
     1381 atcgacgctc gcgtagacga tcgtcgggtt ggcggccgac atggcgagct cgatgcgcat
     1441 gccggaacgc gcgttcggcg ggacagcagg aaagcccgtt gcgggggtcc acgtgctgcc
     1501 tgcatcacgt gacaccatcg cagtgccgag gatgcttccg gccacacaat gctgggcgac
     1561 gctccggctg cactcgacct ggggcatgtg cacctgcgcg tgcggttggc ccgcccgcgt
     1621 gaaggtgacg gcctctgcgt tctgatcgcg atagttggtc gagcggtaga tcccggaatt
     1681 ggtcgcgatc agcatcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcgag cggaagacgc
     1801 cgaggccggg cccgaactcg ctccggcccc agaactcgcc cgtgcccgca tagatgatat
     1861 tcgggttgga cctgtcgagc gcgagcgtcg tcacattgat gctcgccatg aagtcggcga
     1921 gcggcgccca gctctggccc gcattgatgg atttccatac gccacccgag acgcccgccg
     1981 catacatgac attgggatct tgcgggttga tcacgatagc gcgcgtccgc ccgcctatgt
     2041 tcgtcggacc gatccagttc cagcgccgcg gcgagagatc accgggctcc cggttgcgca
     2101 tagcggtgag cgggaggatc ggctcgaacg agagcgtgtc catgccagcg gcgcgcgcat
     2161 ctccggtcgc agccacccgc ggcccggtat ctgcgacggg tatgccggcg atctcgatca
     2221 cctgtcctgc ggcagtcgtc cgcacttcgg cgccgaccgg ccgcgtcacc gccgaaagcg
     2281 cacgtgcacg gccgatcgcc gtctggctca cggcgcgctc gatggcgtcg acgcgccgct
     2341 tgccatcctt cctgccggtt tccatgagcg ccatgtactc gccggcgccg agcgggttct
     2401 cgggcagtgg agcctggatg cgtccgagaa tcttgatcgc ccggtcgcgg gtcatcagcg
     2461 cgagctgttc gagcctgatg ccgcgcttct cccggatgag tcggacctcc tcgacggaga
     2521 tcatcaggcg cttggcaacg tctacctcgg tcggggcttg agccgcggcc atcgaaatgg
     2581 caacggcggt ggaaagcgca atcgtgacaa tgagggagcg cgactgcatg atctcgactc
     2641 cgaacatgga tagaatagtc cgagcttagg ctccggaagc gtccatcagc aatcgatatt
     2701 gtgtttcagc tttgcattcg aactctcacc gagatcaagt gggccgaaag aaatgggccc
     2761 gacgtccacg cgtcgagccc ctccattctg cgcgctgagt ttagagtgtc agctccttca
     2821 gacgaccgtc acctcgagcg tgtcgacgcc ctcgacgtaa cccttgagct tgtcgccctt
     2881 tacggtcgcg cccacgcctg ccggtgtccc cgacatgatg atgtcgccgg ctcgcatctc
     2941 gaacagcgag gagagaatgg cgatctgctg cggcacgctc cagatcatca tcgagaggtc
     3001 ggccgtctgg cgcttctgcc cattgacctc gagccagatc gcgcccttgg acggatgccc
     3061 gatctcggac gcgggcacca gcggcgtcgc cggcgcggag tgctcgaagg ccttgccgac
     3121 ctcccagggc cggccgagct tcttggcttc gctctgcagg tcgcgccgcg tcatgtcgag
     3181 gccaacgccg tagccccaga cgtgattgag cgcatcctcg gccttgatgt ttgtgccgcc
     3241 tgacttcagg gccaccagca tctcgatctc gaaatgcacg tccttggtgc cggcgggata
     3301 gggaaagcgt ccgtcgaggc ggatgttgtc cgggttcttc aggaagaaga acggtggctc
     3361 cttgttgggg tcgtggccca tctcgcgggc gtgctcggca tagttgcggc caatgcagta
     3421 gatgcggtgc actggaaaga gcttgtcggt gccgcggatc ggcagagcgg cctgtgctgg
     3481 cggggcaatc acgtagttcg gcatggctgg ctcctggctc ggctgcatcg gttattgtcg
     3541 atgtcgatcg tctaccagat cgcccgaagc gctgccaccc ggagcccgca atcaacgatt
     3601 gtcggcggcg agcggcatgg tatcgaaggc gttgaacctc aatcctgaac gcagggtcac
     3661 agcacatggc acgcgcggcg gcaaagaaaa gcaaatgcct cgatcaccag gaacggcttg
     3721 accggctcgc tcacatcgcg gttcgcgtcg gcctcaatct gaagcgcggg caggacgtca
     3781 tcgtcacggc gccggtcgag ggtgtggatc tcgcgagact ggtcgtgaag catgcctatg
     3841 aggcaggcgc cggcctcgtc accgtcctgt acgacgatga agaagcaacg ctgctccgct
     3901 acaagcatgc cgccaacgcc agtttcgaca aagcgccggg ctggctccat gacggtgtcg
     3961 ccaaggctta cgagcagggc gccggacgtc tcgccatccg cggcggcaat ccctcgctgc
     4021 tcgcggggca ggacccggcc aaggtctctc gcgtcaaccg cgcccagtcg attgccgcga
     4081 agccgatgat ggagcgcatc accggcttcg acatcaactg gacgatcgtc ccctatgctt
     4141 cggcggcgtg ggcccgcgcg gtattcccag aggccagcga gacgaccgca gtcaggaagc
     4201 tctggaacgc gctcttcatg acgacacgtg tcgacgcgga cgatccggtc gcggcctggt
     4261 cggagcacaa caagcagctc gcgcgccggt cgaaatggct caatcagagg agcttcgcga
     4321 gcctccactt caaggggcct ggcaccgacc tcacggtcgg cctcggcgat ggtcatgtct
     4381 ggtgcggcgg cgccgagcgg gcgaagaacg gcatcgtctg caatccgaac atgccgacgg
     4441 aggaggtctt cactgcgccg cacaaggatc gggtcgaggg gacggtcacg gcaacgaagc
     4501 cgctctccta catgggcacg ctcatcgagg agatcgcggt gcgtttcgag aagggcgcca
     4561 tcgtcgatgc caaggccaag aagggcggcg atgtcctgca caaggttctc gagaccgatg
     4621 atggcgcgcg ccgcctcggt gaggtggcgc tggtccccta cacctcgccg atctcgaaga
     4681 gcgggctcct gttctacaac acgctcttcg acgagaacgc cgcgagccac atcgcactcg
     4741 gccaggctta cacgtcgacg ttcaaggatg gtgccgccga aagcccggcc agactggccg
     4801 gtcgcggcgc caaccgcagc ctcatccaca tcgactggat gatcggctcc aacaaggtgg
     4861 acgtcgacgg catcgcagcg gacggctctg ccgagccggt gatgcgcggc ggcgaatggg
     4921 tttcggcctg atcgatcgga acgaccaatg ctgagcgcgc tcgcccgcag cgatttcccg
     4981 gcctacctcg cgcgcctccg cgcaatcgtg cgcgaggagc tgatcccagc cgagagccgg
     5041 ctttcggacg atgccggttg cccggaagac atggccgagc gcctccgcga gctcggcctc
     5101 ttcggcatga cgatcccgga acgctttggc gggatcgggc tcacaatgga gcagcaggtc
     5161 gaggcaacgc tcgccctcac ctatgcctcg gcatgctacc gttcgcgcgt ctcgacgacg
     5221 ctcgggctct gctcgcaggc gatcctcttc aacggtactg aagcgcaatg cgcgcgctat
     5281 ttgcctgcga tggcgagcgg ccggctgacg gccgccttcg cgctgaccga ggagggcgcg
     5341 ggctccgatg cgggtgccgt cgcgacccga gctgacaagg acggggccga ctacgtcctc
     5401 accggccgga agcgctacat caccaatgcc aacatcgcgg ggctgttcgt cgtgatcgcc
     5461 cgcgtcgatg ccgcgcgcgc gggcccggca gggctggcgg ccttcctcgt cgagcgcggc
     5521 acgccgggtc tttccaccgg tccggtcgac cgcaagatgg ggcaagccgg cgcgcccacg
     5581 gcggaggtct acctcgagcg ctgccgcgtg cccgcgacgg ccctcctcgg cggcaaggag
     5641 ggtgacggct tcaagaacgc gatggccggc atcaacaccg cacggctcca cgtcgccgcg
     5701 acctgcgtcg gccaatccga gcggctgctc gacgaggcgc tggcctatgc ccgctcgcgg
     5761 cgccagttcg gccagccgat cgccgagttc cagtcggtcg gcaacatgct tgcaacgatg
     5821 cgcgccgaga gccttgccgg ccgtgccatg gtggtcgaca cggcgcgccg attccgatcg
     5881 ccgcccgacg caggcgtcgt tgccgatatc gccgcctgca aactctacgc gtccgagatg
     5941 gtctgccgtg tcgccgatca ggcggtgcag atccacggcg gcgcgggcta catggagcca
     6001 tccgatcgag cggctttatc gcgacgtgcg cctgttccgc ctgttcgagg ggacgagcca
     6061 gatccagcag ctcgtcatcg cccgtcatct cgtcgcccat ggccggccgc cggcatgatc
     6121 ggccgcattg ggcgagcgat ctatgacaac gcttacttcc tgctcgcggc cacatcgctg
     6181 ttctgggcgg gcaaccagat cgtcggtcgc atggtcgccg ggcacgtgcc gccggtgacg
     6241 ctcggctttt cgcgctggct gctggcatcg ctgatcatca cgccgctcgc ctggccccat
     6301 ttgacgcgtg actggtcgat cgtgcgcgca aacctgccgc tgctggcctt cctcggcgtc
     6361 atcggcggcg gtacgctcaa cacgctccag tacatcggcc tcaactacac gaccgcgctc
     6421 aacgccctcg tcctcaactc ggcggcgccg gtactcatcg ccgtcgcctg cacgctgatc
     6481 tttcgcgacc ggctgacgcg cctgcagtcg ctcggcatcg ccatctcgct cgccggcgtg
     6541 ctggcgatca tcgcacgcgg cgaccccgcc ggcctgctcg ccctctcgtt caatcgcggg
     6601 gacgcgatca tcttcgtcgg catggtaagc tgggcggtct acacggcgtt cctgcgcaag
     6661 cggcccgcac tgcatccttt tgccttcgtc gccgtcatgt tctgggcggc ggcactggtc
     6721 aacctgccgt tcgcggcact cgagctcgca gggggccagc gcctgcatcc cgatctgaag
     6781 acgacggccg ctatccttta cgtcggcctc ttcgcgggcg tgctcgccta tctcgcctat
     6841 gtgcgcggcg tggaactgat cggcggcccg cgcgccggcg ccttcacgca catgatcccc
     6901 ttcttcgggg caatattggc catgctgttc ctgaacgaag ccttggccag ctaccacttg
     6961 tacggacttg ccctgatcct cttcggcgta tggcttgcag cacggcccaa gtgaagatcg
     7021 ttcaatactg tccacgccca ggagaaacta tttccctgat tttcgcccaa ttctggaggc
     7081 atcaagacgg aaggaggatt gtcatgaccg ctgacgatcg ggccttcgat ctgggccgag
     7141 ccgatttcgt gtcgactgca ccgcctgccg cggggcaaat cgaccttcgg ctcatcgccg
     7201 ccgttgctgc agtgcgcgac gacgagccga tgatactgac agtgcgagga gccgacggcg
     7261 atcagcttcc tgcaggaggc ttcacgccgt cgatggaccg aacgctcgag gcgggcgtcc
     7321 gttcaaccgt ttctcgcgat gccggtctcg atctcgggca cgtcgagcaa ctggtcacgc
     7381 tgagcgatca ccgagcatcg ccgtacgcgc cgccggcatc gagccatcgc atctctgttg
     7441 cctaccttgc tcttgcacga atgggtgaga gcagcgaggg acacggggcg agcaggagct
     7501 ggaagagctg gtatacctat ctcccctggg aagactggcg gcagggacgg ccgacaatcc
     7561 tcgacgagca aatcattccg cgcctggccg cctgggcgca ggagccggac tttggccgca
     7621 gcaatgcaac ggcgctcggc cgcggcgagc gagtcaagat cgccttcgcg acgggtgagt
     7681 cggcctggga cgaggagcgc gtcctcgagc gctacgagct gctctttgaa agcggtctcc
     7741 tcgaggaggc acgcgccgag gggcggcgcg ccccgctcgg accgaaaatg tcggcgctcg
     7801 ggcgcccgat gcagttcgat catcgccgca tactggcgac cgccatctcg cgcgtgcgag
     7861 gcaagatcaa gtcgcggccg atcgtatttg agatgatggc cgccgaattc acgctgttcg
     7921 agctgcagcg cacggtggaa gcactgctcg gcccccacct tcacaagcag aacttccgac
     7981 gcctcatcga ggccggccgc ctcgtcgagc cgaccggatc ggtgcgcagc cgcaccggag
     8041 gccggcccgc caagcttttc cgcttccgca gcgaggtcgt cgtcgagcgc gccgctccgg
     8101 gcgtacgcgt gcgaccgccg cgctgagagg cctttgcttt caggcgatct cgagtgccag
     8161 ctgcacgggc ttgaacgagc gccggtgctg ctccgtcaca ccgagccggc ggagcgcatc
     8221 ctggtgctcg ggtgtgccgt agcccttgtg gcgctcgaag ccgtagcctg gcagctcgcg
     8281 ggcaagcgca atcatcagcc ggtcgcgcgt caccttggcg atgatcgacg ccgccgcgat
     8341 cgacaggcaa cgggcatcac ctttgacgat cgtgcgtgtc ggacaggcga gatgcggcgc
     8401 gcgattgccg tcgatcagcg cgaggtgggg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcctcgcggt cgtcggcatc
     8581 gatcgccttg ctgtcggcga tgccggccgg aatgcgggcc ggatcgagga tcaccgctgc
     8641 cgcaactacg ggcccggccc acggtccacg gcctgcctca tcgatgccgg cgatcgggcc
     8701 acgaccgagc cgcaactctg cctcttcgag ctcgaatgtc gccttgatca tcgcgaatca
     8761 cccccgcgtt ggggcgaatg atcgagccaa ctggcacgcg tgtcaaagtc ggcagttcag
     8821 ctcatgttga ccgccctacc gcccgccagc cgatatcgcg gcggcagaag ccttcgggcc
     8881 actggatgcg gtcgaccgcg gcataggcct tctcgcgggc ctcggcgacc gtcttgccgc
     8941 gcgccgcgac gccgagcacg cgaccgccgt tggcgaggat gcggtcgccg tcgcgcttcg
     9001 tgccggcatg gaaaacctcg acgtcgggca ccgttgtagc ctcggcaagg ccgcgaatct
     9061 ccgtgccacg ccgcggctcg cccgggtagc cctcagccgc catgaccacg acaagtgcgg
     9121 gctcgtcatg ccagcgcaga tcgaacgtgc cgagcacccc gtcggcggtc gcgagcagcg
     9181 ccgccagaac gtcggacttg agccgcatca tcaggacctg gcactccgga tcgccgaagc
     9241 ggcagttgta ctcgtagatc ttcgggccgg ccggcgtgat catgatgccc gcgaaaagca
     9301 cgcccttgaa cggcgtgccg cgccgcgcca tggccgcgac cgtcggccgg atgatctcgg
     9361 ccatgatgcg cttgtggatg gccggcgtca cgacgggcgc cggcgagtag gcgcccatgc
     9421 cgcccgtgtt ggggcccgtg tcgccgtcgc cgacgcgctt gtgatcctgg gctgtcgcga
     9481 gcgacagcac gctcgtcccg tcgcagagcg cgaagaagct cacctcctcg ccctcgagaa
     9541 actcctcgat cacgacctcc gcgcccgccg aaccgaaagc gcctgcgaag caggcctcga
     9601 cggccgcctc ggccatgtcg cgtgtcgtcg cgactaccac gcccttgccg gccgcgagcc
     9661 catctgcctt gacgacgatc ggaaccggtt gtcgggcgag gtaggccatg gcggcagcct
     9721 tgtcggagaa gcggccgtag gcgcccgtcg ggatgccgac ctcggcgcag agatccttgg
     9781 tgaagccctt cgagccctcg agtgctgccg cggccttcga tgggccgaag tgtttgatgc
     9841 ctgccgccgc gagatcgtcg gcgagaccgg cgacgagcgg cgcctcaggt ccgatcacca
     9901 cgaagtcaat gcgcgtctcc cggcagaagc ggatgatctc gtcgtgcttc gagacgttga
     9961 gcggcacgca ggtcgcgacc tcggcgatgc cggcgttgcc cggcgccaca tagacgcggg
    10021 tcgcgagcgg actagcgaca atcgcccagg cgagcgcatg ctcccggccg ccggagccga
    10081 tgaccagcac attcatgacg agcctccgcg gttgcctcag gcctttttgc cgagcacccg
    10141 cccggcgacg gcgtcgagct tggcgaccag cgccggatcg cgcttgtcgg gggccgtaat
    10201 gatggcgacg tcgagcgcat gatccgagcc gacggggcac ttcgggtgct cgcgcgggaa
    10261 ctcgcgggca agccgcgtga cgaggcgctg cgccttggcg acattggcgg tcagcacctt
    10321 tatgacggcg gcgacgtcca catcatcgtg gtcctcgtgc cagcagtcgt agtcggtgac
    10381 catggcgacg gtcgcataag ggagctctgc ctcgcgcgcg agcttggctt ccggcatgtt
    10441 ggtcatgccg atcacggagc agccccagct ccggtagagg ttcgattccg cgcgcgtcga
    10501 gaactgcggg ccttccatga cgatgtaggt gccgccgcgc gtcgcggcga tgccctcggc
    10561 attcgcggct ttctcgatga gatcgacgag gccggggctc acgggatcgg cgagcgccac
    10621 gtgggcaacg caacccgtgc cgaagaagct cttctcgcgc gcgaacgtgc gatcgatgaa
    10681 ctggtcgacg agcacgaaat ggccgggcgg gaactcctcc ttgagggagc cgcaagccga
    10741 gatcgagatg agatcggtga caccggcccg cttcagggcg tcgatgttgg cgcgatagtt
    10801 gatcgcggag ggaggtgtcc cgtggccgcg tccatgtcgc ggcaggaaac ggatcggcag
    10861 gccagcaacc tcggcaaaga ggatcgcatc cgagggctcg ccccacgggc tcgcgaccgc
    10921 ctgccagcgt ggattttcga aggccggcag atcatagaga ccgctgccac cgatgatgcc
    10981 gagcaccgat ttggtcatgg tgtccttcca gagcgcgaga accgagctgc caaaactgat
    11041 gtgggccgca cggcggcggc ccacaagcga aatctgtggc tcgcgggggg atcagatctt
    11101 gcgatcgccg gggagcggcg gtggcgtgtt cggcgccttc gggttcggca ccgcacccgg
    11161 cgcacccggc cgcaccggcg gcacatacat gcgcgcggcc gagcagaggc gcgactggtt
    11221 ctcgttgttg ccgcaggcac gataggcgcc atcgcgcgtg aagcagatgc gcacctcttt
    11281 caggcggtcg cccggacgcg agcactgcac ggcaatcgat tcggtcttca ggcccggatt
    11341 cgctgcgaca aaggccgcga tcagctcggc ggggctcacg aactgcgccg cagcaggctc
    11401 atcgaaccgg tcggggatct tcaccttgga aaagagtttg cggctcgtct cgaagtagcc
    11461 ttccggcgag aggcccgagc aggtgccgtg cttcctgtac tcgtggatca cgagcccgcg
    11521 cgccggcatg atgtcgagca tgcgggtgat gagcgcatcc ggcacgaacg gccgctcgcg
    11581 cgtctggcac gactcgggga agccgcgctc gtactgcggc cagaggccat gcagcacgaa
    11641 ggagtagcgc ttgttgttgc gcggccggca ctgcatgtcg tcgcgccccg gggtcgcggt
    11701 cgcgcagtgc gtcgggctcc aggagaggac gagcgcgtag tagtcgaact ggccagcgac
    11761 gtgggacttg ggcgcgccga acggcgccgg accaggacca ggtccgcgct gcgccagcac
    11821 ggcggtcgtg gtgccaatcg cgagcgcgag gcccgccaag gcgaaacttt tcaatctcat
    11881 gaggcgcgtc catccgctgg ttgttggcgt cgagcgtgac cccgttccgg cgcggccgga
    11941 cgattgcggc ggatcatagc ggccattgag gccgaggcaa ggcccgtcgg tgaatgcatc
    12001 tcctggccca atgattggga gaacgcggcg cgagccctcg aggcctgatg ccccctacac
    12061 acactcccga tcaaggccta agctcgcaaa catcgcaccc ggagacccgc cgccatgccc
    12121 gtgccgtccc tcatcgagct cgacatctcc gagcgcttcg cgtttgccga tggacatgcg
    12181 ttcggcgacg ccggttccta tgagcggctc aaggggcgcg cgcgctttgc cgtcgatccg
    12241 acggcacagg cgcaagccgg catcgtcgac ctcgacaagg cgccgcgcgg cgccgacggc
    12301 cgcgtcacct tcacggcgga catctcgatc ctgaagccct ccgatctcgc tcgctccaac
    12361 cggcggctct tcttcgactg gggcaaccgc ggcaacatcc gctgcctcca gttcttcaat
    12421 gatgcgccgg gctcgaacga cccgcgcacg cgtgcgcacg ccggcaacgg cttcctgctc
    12481 cgccgcggtt actcgctcgt ctggtgcgcc tggcaggctg acctcctcgc cggcgaccat
    12541 cgcttcctgc tcgacgtgcc ggtggcgacg gacaatggcc gcccggtcac tggccccgtg
    12601 cgcgtcgagt acatcgccga tcaagcgggc atcacgacgt tcccgctctc ctcgcgcgcc
    12661 tcgacgcgga gccacccggc cgtctcgctc gacacgcgca aggcaacgtt gacgcgtcgg
    12721 cgctatcccg gcgatccgcg tcagccaatc ccgcacgacc agtggatgtt cgcccgagtc
    12781 gaaggcggca ccggcctcga caatcagggc gccgagttct cggtgatccc atccgataca
    12841 cacatccaca ttccgaaagg tttcgagacc ggctggatct acgagctcgt ctacgagggc
    12901 cgcgatccgc tcgtgctcgg cctcggccat gtcgcggttc gcgatctggt gagcttcctc
    12961 cggcatggcg agacgggcaa tccgctccgc gccggcagcg tcgccatgga gaaggcctac
    13021 ggctggggcc gttcgcagac cgggcgctgc atccgcgact tcatccaccg cggcttcaat
    13081 gcggatgccg ccggccgtcg cgtcttcgac ggcctgctgc cgcacgtctc cggcgccggc
    13141 ctgatgtggc tgagccgctt cgccaacgtg atcgtcgccg ctggccagca gtacgaggac
    13201 cactacaatc cggccgacca gttcccgttc acctacgcgc cgacgacgaa ccacaacaca
    13261 ggcaagtccg acgcaatcat gaagcggccg gcaaccgatc ccctcgtgct gcacacccag
    13321 accgcgacgg aatattggca gcggcggggc tcgctcgtcc acaccgacac gaagggcaac
    13381 gacgtcgcgc tccccgagaa tgtgcgcgcc tacctctggt cgagctcgca gcacttcgcc
    13441 gatccacggc ttgcgaagcc cgcgcgcggc gtctgccaga acgtcgtcaa cgtggtttgg
    13501 acgtcgatgc tgttccgcgc catgctcgac gcactcgatg cctgggcgac caagggcacg
    13561 ccgccgcctg cgagccggat cccacgccgc agcgacggca cgctcgtcac gttggaggag
    13621 tggcggaaag ccttcccggc gatccccggc gtgatgacgc cgcgctcgcc gagcgaggcg
    13681 ccattgctcg actgggggcc ggaggcgagc cgcggcatcc tcatcgagcc gccacaggtg
    13741 ctcgccgagc aaggctatgc cgtgctcgtg ccgcaggtcg acaaggacgg caacgacatc
    13801 gccggcgtgc gcgcgcctat ggtcgaggcg ccactcgcga cctacacagg ctggaatctc
    13861 cgcacacgcg gcgtcggcca tggcgccatg cacgagttca cgggctcgac actgccgttc
    13921 cccgggacgg cggaggaggc gcgcgccacg ggcgatccgc gccgctccat cgccgagcgc
    13981 tacggcacgc agcgcggcta tgccgaggca atcgtcgcgg ccgcccgacg tctcgtggcc
    14041 gatcgcctga tgctcgagga ggatgtcgag cggtgtgcag cctcggctgc gaactggcac
    14101 gcgccgcgcc acgacgtcgg cctgccgtga tgctgaaccc acctgtgatc gagggtgcgc
    14161 gcgacctgct cgcgcgctac gacgccgtct tctgcgacgt gtggggcgtg ctgcatgacg
    14221 gtcgcgatgc gttcaaatcg gcatgttcgg cgctgatcgc cttcaaggcg cagggtggcg
    14281 ctgtcgtgct cgtgtcgaat gcgccccaac ccaaggagcg cgtcggcgag atcctcgatg
    14341 aacgcaacgt gccgcgcgag gcctgggacg cgatcgtgag ctccggcgac ctggcgctcg
    14401 agcatgtcgc gaaaagcggc tttcggcgga ccgcgcgcat cggcccgctg ccgcgaagct
    14461 tgccgttctt cgcgcgcctt tccggcgagg ccgccccgat cgagacggcc gaggcgatcg
    14521 tctgcacggg cctcgtcgat gatcacaacg agacggccaa agcctatcgg ccgatgctcg
    14581 ccaaggccct ggcgcgccgc ctgccgttca tttgcgccaa cccggatctg atcgtcgacg
    14641 tcggcggccg gttgtatctc tgcgccggcg ccgtcgcgaa actctacgag gagatgggcg
    14701 gcgaggtctt ctgggcgggc aagccgcatc tgccggccta tcaccgggca cgctccgagg
    14761 ccgagcggct gctcgcgcgg tcgataccca agtcatcgat actggcgatc ggcgacgcga
    14821 tgcggaccga tatcgccggc gccgccggct ttggtatcga ttccctgttc gtcgccaacg
    14881 gaatacacag gcacgaggtc accgacaacg gccacatcga tc
//
LOCUS       DAORNH010000081        14777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3083_length_14777_cov_2.203097, whole genome shotgun sequence.
ACCESSION   DAORNH010000081 DAORNH010000000
VERSION     DAORNH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3083_length_14777_cov_2.203097"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>288)
                     /locus_tag="PK264_10165"
     CDS             complement(<1..>288)
                     /locus_tag="PK264_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD76290.1"
                     /translation="VTVPGGPGSGLDQAARGVEDALKAENLAGAIRIANVTGGGGMIA
                     LSQFVTTKEANKTAVVFQGAGTVFFPLTNKTQVSLADVRPLARLAGEYEVMA"
     assembly_gap    289..362
                     /estimated_length=74
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(430..>1656)
                     /locus_tag="PK264_10170"
     CDS             complement(430..>1656)
                     /locus_tag="PK264_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD76291.1"
                     /translation="IDGNAMARKGRGAQALATAAIGSFVAGTIATIGLTFAAPLLVAM
                     AITLTAPDYFALMVLSLATATAVISRPSGGGFIGYGLASLFFGLLLGTVGLDPQTGQA
                     RFAFGVPELLDGIDTVVVAVGLFAVGETLYVAATDGGKPDAMHQMKGSLWLSREDWAR
                     SWKPWLRGTFIGFPIGAIPAGGSEIPTILSYTLEKKLSKNPGEFGQGAIEGVAGPEAA
                     NNASAAGVLSPLLALGLPTSTTAAVLLAAFQQYGLRPGPMLFESNATLVWGLIASLYI
                     GNVFLLVLNLPLVGLWVRLLSIPKPWLYAGILVLASLGTYSLNSNAVDLVLLWGFGII
                     GFAMRVLGVPVVPCILGLILGPLIEQQLRRSLAISQGDWTVFLTQPVSALLLSAAAIL
                     VLWPVFKTFKPRRAPA"
     assembly_gap    1657..1828
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1951..>2296)
                     /locus_tag="PK264_10175"
     CDS             complement(1951..>2296)
                     /locus_tag="PK264_10175"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRD76292.1"
                     /translation="VAGAGLVLSALVILGEAVLRPGAADGDFTGVDVWPVALMSAALL
                     AEAVLIARIGWVPVVTILFATGAWAFGDRRVMSNLLIGFSVGLVVLVAFRFGLGIDLP
                     LGPLAPLLPVTS"
     assembly_gap    2297..2341
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2342..2555)
                     /locus_tag="PK264_10180"
     CDS             complement(<2342..2555)
                     /locus_tag="PK264_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76293.1"
                     /translation="MFKTLELWAVVKSIKSILLTYMRAAVSADSTGRETVPERREGGM
                     TRNMPALLFGSFVLVLGAAVLVVAGSI"
     assembly_gap    2665..2885
                     /estimated_length=221
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2886..>3532
                     /locus_tag="PK264_10185"
     CDS             <2886..>3532
                     /locus_tag="PK264_10185"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HRD76294.1"
                     /translation="VTIPVIADCETGFGNVVNTTRAVHEFISAGVAGLFLEDQVFPKR
                     CGFTKGVEVIGIDEAAGKYRAALAERDRLDPDVVVIARTDARAAVGGGMDEVMQRCEA
                     YLKAGVDMLMVMALQSREEMRRVTEAFPNVPIYINASSVRPLIGHAEYGDVGCALYNI
                     SVAKVAQIMMERFLADCRARGAEAFNDFMASGAATGKSTFGYLNLTAFSAVVAIE"
     assembly_gap    3533..3645
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3646..>3889)
                     /locus_tag="PK264_10190"
     CDS             complement(<3646..>3889)
                     /locus_tag="PK264_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HRD76295.1"
                     /translation="DADARFHHLLIERCENFRFQTIVGTVNAQAHRVRTLTVNLRHSL
                     KDSVTEHRRIIAAVAAGDAGQASGEARGHRARARLEI"
     assembly_gap    3890..3909
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3910..4312)
                     /locus_tag="PK264_10195"
     CDS             complement(<3910..4312)
                     /locus_tag="PK264_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD76296.1"
                     /translation="MASVKTMRSNRSWLVDDAYAALKAAILANDYTSGQHISAQDIAA
                     RLGMSRTPVQEAALRLQQEGLLDILPKRGIRIRSLTHEDISEIYDVLIALEGHAAAML
                     AAQPEEKRRNACARLAEDTDNMAGAIARGDRA"
     gene            4425..5498
                     /locus_tag="PK264_10200"
     CDS             4425..5498
                     /locus_tag="PK264_10200"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRD76297.1"
                     /translation="MKTYRIAAIPGDGIGTEVIDAGVEVLKALAMREAFDLQFDRFDW
                     GGEYYKKHGRMMPEDGRAQIRTHDAILFGSAGHPEIADHITLWGLRLAICQPFDQYAN
                     VRPTRILPGITSPLRKVSGPELDWVIVRENSEGEYSGVGGRVHQGLPEEAATDVSIMT
                     RAGVTRIMRFAFKLARSRPRKLLTVVTKSNAQRHAMVMWDEIAVEVAKEFPDVKWDKM
                     LVDAMTMRMVGKPASIDTVVATNLHADILSDLAAALAGSLGIAPTANLNPERAFPSMF
                     EPIHGSAFDIMGKGVANPIGTFWSAVMLLDHLGETAAGARLMAAIERVTADPRNHTPD
                     LGGTARTKDVTAAVIAAIERGNT"
     gene            complement(5540..6175)
                     /locus_tag="PK264_10205"
     CDS             complement(5540..6175)
                     /locus_tag="PK264_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD76298.1"
                     /translation="MAHVIDWRSLEAASPLVAHMPERLRQAARTVQIPGGGSIFRRGD
                     RPSAMYFVVSGEAHLLRRTPVGHDVILQRARSGFLAEASLDQSAYHCDALAIVDCTLM
                     CLPQKRFSEALSVENFRQSWIAHLSRELRRVRAQAERLSLKTARERVIHYLETEGERA
                     TIVLAHSKKQWAAELGLTHEALYRTLACLTRERLIALEGSTIRITRRGESV"
     gene            6269..6751
                     /locus_tag="PK264_10210"
     CDS             6269..6751
                     /locus_tag="PK264_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76299.1"
                     /translation="MRNSVLAALVAGLIVLVSGLASAQHRGSGDHKGLPSVADARVVV
                     NVPPAMAAHTLANMRDHLLALQEITEALAKGLHDKAARIAEQRLGMSSLGSHGAHEIA
                     KFMPKGMQDAGSAMHRAASRFAIEAQNVGVTGDVRPALATLGEVMSACVGCHAGYRFK
                     "
     gene            complement(6787..8151)
                     /locus_tag="PK264_10215"
     CDS             complement(6787..8151)
                     /locus_tag="PK264_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD76300.1"
                     /translation="MKAAKADQVTTASGAAMQPSLSVETWPSGVEPTSEYAAAIAHVR
                     EGAGNLFITGRAGTGKSTLLRSIVDMVPEERAIVAPTGLAAVNVGGQTIHSFFGLPPR
                     LIRSEDIRRSRNGSVMRRLKHLIIDEVSMVRSDLMWAIDQSLRLNRGRPREPFGGVRL
                     ILFGDLHQLPPVVQEAEVAAHLEATYGGPFFFSVSTLREGAGTALLELTQVFRQRDEA
                     LLRVLNRIRDGDADEADLEALNERVHPIRTLSEGDPYVILTPTNAAANRINLAFLDAL
                     PGKAMSYEAGVTGDYSESAHPTDATLVLKPGAKVILLRNDSDRRWVNGTIARISRLEE
                     KRVFVEIKGSEHEVEQVAWEARRYAFDQAQGKIVETIAGTFRQLPLRLAWALTIHKSQ
                     GLTLDKVYIDLGRGTFAHGQTYVALSRCRTLAGLALARRMTPRDILFDRSAMGYRDLF
                     PTLA"
     gene            complement(8258..8452)
                     /locus_tag="PK264_10220"
     CDS             complement(8258..8452)
                     /locus_tag="PK264_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76301.1"
                     /translation="MLLKAWLLLGLAVVTGWFIWNFVPVLIPMAAVLAGLGAIAFAMV
                     RLARWIERARGLPPRMDQHE"
     gene            complement(8459..9277)
                     /locus_tag="PK264_10225"
     CDS             complement(8459..9277)
                     /locus_tag="PK264_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HRD76302.1"
                     /translation="MTQNTLTLAIAGLGAIGLAVARKVDQGTVPGLKLVAVAARDRAK
                     AERNLASFRAFPENVTLPELAERAEVILECIPARHFAEVAEAAVARGRIFMPLSVGQL
                     LGNMHLVDKAKTTGARIIVPTGALLGLDAVRAVAEGNVESVTISTRKPPKGLIGAPHL
                     IENAISVEGLTAAKKVFEGNAREAIKGFPANVNVAVALSLAGIGPERTRVEVWADPAV
                     TRNTHTIRVKSDSSDLTMTIENIPSAENPATGKVTALSAIAALRRLTAPLVVGT"
     gene            9467..11119
                     /locus_tag="PK264_10230"
     CDS             9467..11119
                     /locus_tag="PK264_10230"
                     /inference="COORDINATES: protein motif:HMM:NF006122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD76303.1"
                     /translation="MAQTNLAKLNAAEVVVKTLIANGIKELYCLPGVQNDDFFDALAR
                     IGGAIRPIHTRHEQATAYMALGAALATGKPQAYCVVPGIGFLNASTPLATAQSLTQPV
                     LCISGHIVERYIGKGYGYLHEIPNQLGIMKTLTKWAEHIGGAEDAGPKIREAFRQLNS
                     GVPGPVGISCGWDRWKHAGEVAFDDEAATRRTPAIDLDQVKAAAKLLGKAKKPLIVVG
                     GGAQGASAEVTALANMLEAPVAANRTGQGVLDSRHHLAIRNVYAHRIWKDVDVVLAIG
                     TRLQQHQIDWGLDDDIKVIHLDIDPTRIGRITKPEVGIIADAAEGVRALIDALAKHNM
                     KRASRKEEMAELKARVGKELEVLAPQKAWVDAIRRTVPEDGIVVEELTQISYASRFLY
                     PVYKPRTYISSGYQGTLGWGLSTALGVKVAMPDRAVLSINGDGGFMFGVQELATAVRH
                     NIPLVSVVFTDDAYGNVRRMQAANYEGRVIASDLKNPDFVKLGESFGVRSERAKTPDA
                     LAKALGTAFKAKEPSLIEVPVGEFPDPWKLVFLGRVRGTKPG"
     gene            complement(11097..11990)
                     /locus_tag="PK264_10235"
     CDS             complement(11097..11990)
                     /locus_tag="PK264_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD76304.1"
                     /translation="MTRFRADLILLGCAVIWGFAFLFQKTAMEHVGPLTFVASRAALA
                     AVVLAPLAYSELRRKGLALDRDILCHGAFCGLLFFSGAAFQQLGLTTATVTNAGFLTA
                     LYVILVPFIAWVTLGKLPASVVWPAAAMSFAGTWLLGGGTLGHFSQGDLWIIVCAFFW
                     AGHVVSLSRPTALARPLTLMCVQFATVFALAATGALALETVAFAGVMAATPQILYLGV
                     LAGAITFTAFTYAMRATPAAEAAVIVSLETLFAAIAGYMFLNERLGLVGWLGAALIVS
                     AGLLVQLSPLVSRHLSQASSP"
     gene            complement(11987..>12513)
                     /locus_tag="PK264_10240"
     CDS             complement(11987..>12513)
                     /locus_tag="PK264_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HRD76305.1"
                     /translation="NPPHVVPIVELCGAPWTSPDVVARARAIYQSAGQVPITVKKEVD
                     GFILNRLQVALLTEAFRLVQEGYVSPRDLDHTIADGLGLRWAFMGPFETIELNAPEGI
                     ADYCTRYVEWFRRYMKDMPPASVWDDASWKKAAESWGPAPPREAIRRKSQWRDERLAA
                     LVAHKRAQKRHQDR"
     assembly_gap    12514..12523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12524..12883)
                     /locus_tag="PK264_10245"
     CDS             complement(<12524..12883)
                     /locus_tag="PK264_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRD76306.1"
                     /translation="MFARSGFDVRLWDPHEATLREARDLIAASLDELARHGLVADPAG
                     AAKRITTCTSMSEAVAGAEIVQENGPERLDAKLAIFAELDRLAPATAILASSSSAIVA
                     SKFTEGLAGRSRCLIAHP"
     assembly_gap    12888..12923
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13282..13316
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13317..13924
                     /locus_tag="PK264_10250"
     CDS             <13317..13924
                     /locus_tag="PK264_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76307.1"
                     /translation="DAVDHQIRAKAMPPPVVMTRWDDNSVVFPITGFDKAGKRASFNV
                     LLLTKEYAWVRGSTGELVKGGRVLAAGDVLKEIFSERVRQGLKPSKQLIAVGIASQEG
                     DPDVELKRAGDRAKQTADWTRAALGAEVELWTLNLGQYRIACTGCETGDTNWQRPFVL
                     IAVRDQEAAVVIAEALANAMTDRANVPSPESYSAFRMERLK"
     gene            14001..14549
                     /locus_tag="PK264_10255"
     CDS             14001..14549
                     /locus_tag="PK264_10255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD76308.1"
                     /translation="MSTVITILGVAALAIAGILMVASRRPDTFRVERSAQVAAPIANV
                     YPRIASLRAMNEWNPFVSPDPDIKITYSGPDLGRGAAHTWDGNRHVGAGHIEVIEAEA
                     PAKVVMRLVMVKPIAADNRVEFTLEPSGDGTKVTWAMSGRQPLLAKCMTLFYDCDRMV
                     GGRFEAGLAGLKRIAEGGSRAS"
     gene            complement(14669..>14777)
                     /locus_tag="PK264_10260"
     CDS             complement(14669..>14777)
                     /locus_tag="PK264_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRD76309.1"
                     /translation="RETGESKGYNASFTKADRSRFLQALEEAMRATKRS"
BASE COUNT         2660 a         4625 c         4539 g         2227 t
ORIGIN      
        1 cgccatcacc tcgtattcac cggcaagacg ggcaaggggg cgcacatcgg ccagtgaaac
       61 ctgcgtcttg ttggtgagcg gaaagaacac tgtccccgca ccctggaaga cgacagcagt
      121 cttgttcgcc tctttggtcg tcacgaactg cgatagcgcg atcatgcctc cgccgcccgt
      181 gacattggcg atccggattg caccggcaag gttttcggcc ttcagggcat cctcaacccc
      241 acgcgcggcc tgatcgaggc cactgcccgg cccgcccggc accgtgacnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nngaccggca agggtctcgc gcctgtcaat catcatggta tcctcccgaa gatggtcttg
      421 ttgtgcgcct catgccgggg cgcgacgggg tttgaacgtc ttgaagaccg gccagagcac
      481 cagaatcgcc gcagcggaca acagcagagc ggagaccggt tgcgtcagga acaccgtcca
      541 gtcaccctgg ctgatcgcca gcgagcggcg cagttgctgt tcaatgaggg gaccaagaat
      601 aagcccgaga atgcagggca ccaccggcac accgagcacg cgcatcgcaa acccgatgat
      661 accgaaaccc cacagcagca cgagatcaac ggcattggag ttgaggctgt acgtcccgag
      721 cgaggccagc accagaatcc ctgcataaag ccagggtttc gggatggaca acagccgcac
      781 ccacagcccg accagcggca ggttgaggac cagcagaaag acattgccaa tgtagagaga
      841 ggcgatcaga ccccagacaa gcgtggcgtt ggattcgaac agcatcggtc cgggacgcag
      901 accatattgc tggaatgcgg caagcagaac cgctgctgtg gtcgacgttg gcaggccaag
      961 cgccagcaac ggggacagaa cgccggcagc ggaggcattg ttggcagcct ccggaccagc
     1021 cacgccctcg atggcgccct gcccgaactc gcccgggttc ttcgacagct tcttttcaag
     1081 cgtataggac aggatcgtcg ggatttccga cccaccggcc ggaatagccc cgatgggaaa
     1141 gccgataaac gtaccccgca gccacggctt ccacgaccgc gcccagtctt cacgactgag
     1201 ccatagcgaa cccttcatct gatgcatggc atcgggcttg ccaccgtccg tggccgcaac
     1261 atagagcgtc tctccgacag caaacagccc gaccgccacg accaccgtat cgatgccgtc
     1321 cagcagttcc gggaccccga aagcaaaacg tgcctgcccg gtttgcggat caagccccac
     1381 tgtccccagc agcaatccaa aaaacagcga agccagccca tagccgatga acccgccgcc
     1441 ggacggacgg ctgatcacgg ctgtggctgt ggccagcgac agcaccatca gggcgaaata
     1501 gtcgggcgcc gtcagcgtga tcgccatggc aaccagcagc ggcgcggcaa aggtcagtcc
     1561 gattgtggcg atcgttccgg caacaaacga cccgatcgca gcggtcgcca gagcctgcgc
     1621 gccgcgcccc ttgcgcgcca tcgcattgcc atcgatnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ccggcaggac accgatcgcg gtgccaagcg
     1861 tgacaccgac catcgaccac acaaggttgg ctggcgtgag tgcgaccgca aagccggcca
     1921 gaagatgcgt cagcgtatcc atgccgcacc tcagctcgtt accgggagca ggggagccag
     1981 cggccccaga ggcaggtcaa tgccaagccc gaaacggaaa gcgaccagaa ccaccaggcc
     2041 gacgctgaat cctatcagga gattggacat cacccgcctg tcaccgaacg cccaggcacc
     2101 cgtcgcgaac agtatcgtca caaccggaac ccacccgatc cgtgcgatca ggacagcttc
     2161 ggccagcagg gctgccgaca tcagcgccac aggccagacg tcgacacccg tgaaatcacc
     2221 atcagccgca cccggacgaa ggacggcctc gccaaggatg accagcgccg agagtaccag
     2281 accggcaccg gcaaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 ngaatactgc ctgccaccac gagcacggct gcaccaagca caagaacaaa gctgccaaag
     2401 agcaacgctg gcatgttccg cgtcatcccg ccctcccggc gctccggcac ggtctcccgg
     2461 cctgtgctgt cagcgctaac agcggcgcgc atatatgtca acaaaattga ctttatagat
     2521 ttcacaaccg cccataattc aagggttttg aacatttctg ttgacactta gtctgtaccg
     2581 tcgttccctg aagctccgaa acagtcgcca gacgaacgag gagacgcccc gtgcatctca
     2641 gacccaccgt gtgcgagggc cgtannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnccgtg accattccgg tcattgccga ctgcgagacc gggttcggca atgtcgtcaa
     2941 cacaacacgt gccgtgcatg agttcatcag cgccggcgtc gccggattat tccttgagga
     3001 tcaggtgttt cccaaacgtt gcggcttcac caaaggtgtc gaggtcatcg gcattgacga
     3061 ggcggcagga aagtaccgcg ctgcgctggc cgagcgagac agacttgatc ctgatgtcgt
     3121 ggtcatcgca aggaccgacg cgcgcgccgc tgtcggaggc ggaatggacg aggtgatgca
     3181 acgctgtgag gcctatctca aggccggtgt cgacatgctg atggtgatgg ccctgcagag
     3241 ccgcgaggaa atgcgccgtg tcacggaggc attcccgaat gtcccgatct acatcaacgc
     3301 cagttctgtg cgtccgctga tcggccacgc tgagtatggt gacgttggtt gcgccctcta
     3361 caatatctct gtcgccaagg tggcacagat catgatggaa cggtttcttg ccgattgccg
     3421 cgcacgcggc gccgaggctt tcaacgactt catggcgtct ggcgccgcca ccggcaaatc
     3481 caccttcggc tacctcaatc tcaccgcctt ttccgcggtt gtcgcgatcg agnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggatt tcaagccggg
     3661 cgcgggcccg gtgccctcgc gcctcccccg aagcctgccc ggcatcgccg gccgcaacag
     3721 ccgcaatgat ccggcgatgc tccgtgacgg agtccttgag gctgtgacgc aggttcacag
     3781 tgagcgtacg gacgcgatgg gcctgcgcat tgacggttcc cacgatggtc tggaacctga
     3841 agttttcgca tcgttcgatg agtagatgat gaaaccgtgc gtcagcatcn nnnnnnnnnn
     3901 nnnnnnnnnc ggcccggtca ccacgggcaa tggcaccggc catgttgtcg gtgtcttccg
     3961 caagacgggc gcaagcgtta cggcgcttct cctctggctg tgcggccagc atcgcggcgg
     4021 catggccttc cagcgcgatc agaacatcat aaatctcgga tatatcctcg tgtgtcaggc
     4081 tgcggattcg aatgccacgc ttgggcagga tatcgagcag cccctcctgc tgcaatcgca
     4141 gcgccgcttc ctgaacgggc gtccggctca tccccagccg cgcggcaatg tcctgagccg
     4201 agatgtgctg gcctgacgtg tagtcattgg ccagaatggc ggccttgagc gcagcatagg
     4261 cgtcatcaac aagccaggac cggtttgaac gcatcgtttt cacgctggcc acaccctccg
     4321 gacctccgtt cccgcttcta ccagcctgcc acaaccttga ccagtcagcc tgcgttgaca
     4381 tcttccatgt aatgttccat ggaagaaagg aataaggaat gatcatgaaa acctatcgta
     4441 ttgctgccat tcccggcgat ggcattggca ctgaagtgat cgacgcaggc gtcgaagtcc
     4501 tgaaggctct cgccatgcgc gaagcattcg atctgcaatt cgaccgtttt gactggggcg
     4561 gcgagtatta taagaaacac ggacgcatga tgcccgagga tggccgtgca cagatcagga
     4621 ctcacgacgc gattctgttc ggctcggccg ggcatcccga aattgccgat cacatcaccc
     4681 tgtggggctt gcggctggcg atctgccagc ccttcgacca atacgcgaat gtacggccga
     4741 cgcggatcct tcctggaatt acatcgccgc tgcgcaaggt gagtggtccg gaactggact
     4801 gggtgatcgt gcgggaaaat tccgaaggcg aatattccgg agtcggtggc cgtgtgcatc
     4861 agggattgcc ggaagaagcg gcaaccgatg tatcgatcat gacccgcgca ggcgtgaccc
     4921 gcatcatgcg cttcgcattc aagctcgcgc gctcacggcc gcgcaagctg ctgacggtcg
     4981 tgacgaagtc gaacgcgcag cgccacgcta tggtcatgtg ggacgagatc gccgtcgagg
     5041 tcgccaaaga gttcccggac gtcaagtggg acaagatgct ggtcgatgcc atgaccatgc
     5101 gcatggtggg caagcccgcc tcgatcgaca ctgtcgtcgc caccaacctg cacgccgaca
     5161 tcctctcgga ccttgcggcg gcactcgccg gctcgctcgg catcgcaccg acggcaaacc
     5221 tcaatcccga gcgcgccttt ccctcgatgt tcgagccgat ccatggctcc gccttcgaca
     5281 tcatgggcaa gggcgtcgcc aatccgatcg gcacattctg gtcggcggtg atgctgctcg
     5341 atcacctcgg cgagacggca gccggtgcgc gactcatggc cgcgatcgag cgcgtgacgg
     5401 cggacccgcg caatcacacg ccagatctag gcggcacggc gcgcaccaag gacgtcaccg
     5461 cggccgtgat cgccgcaatc gagcgcggca atacctgaaa cgctgcgacg aggggttgcc
     5521 attgcggagc aaggcgggct cagacactct cgcctcggcg agtgatccgg atcgtcgagc
     5581 cctccagcgc gataaggcgc tcgcgcgtca ggcacgctag tgtccgatag agcgcctcgt
     5641 gggtcaggcc aagctcagcg gcccactgct tcttcgaatg cgcaagcacg atagtggcac
     5701 gctcgccctc ggtctcgaga tagtggatga cgcgctcgcg tgcggtcttg aggctcaggc
     5761 gctccgcttg agcgcgcacg cgccgcaact cccgggacag atgggcaatc cagctctgac
     5821 ggaagttttc gacggacaga gcctcgctga accgcttctg cggcaggcac atgagtgtgc
     5881 agtcgacaat tgccagtgca tcacaatggt aggccgactg gtcgagacta gcctccgcca
     5941 gaaagcctga tcgcgcacgc tgcaggatca cgtcatgacc gaccggtgtc cggcggagga
     6001 ggtgcgcctc gcccgatacg acgaagtaca tggcggaagg cctgtcgccg cgcctgaaaa
     6061 tgctgccgcc gcccgggatc tgaaccgtgc gagccgcctg ccgaagccgc tcgggcatgt
     6121 gagcgacgag aggcgaggct gcctcgagcg agcgccagtc gataacatgt gccatgtccg
     6181 ttgccgcgcg gctgcttatg atctcaatca tatggtgacg gtacgcccgc ggtctatgga
     6241 tcggcccttg ccatcaagga gcctgcacat gcgcaattcc gttcttgccg cgctcgtcgc
     6301 cggactgatc gttctggtga gcggacttgc ctcggcccag catcgcggct ccggcgacca
     6361 caaaggactt ccgtcggttg cggacgcgcg agtggtcgtc aatgtcccgc cggccatggc
     6421 ggcgcacacc ctcgccaaca tgcgcgacca cctcctcgcc cttcaggaga tcacggaagc
     6481 attggccaag ggcctgcacg acaaggcggc cagaatcgca gagcagcgcc taggcatgag
     6541 ttcgctcgga tcgcacggcg cgcatgagat cgcgaagttc atgccgaagg gtatgcagga
     6601 cgccggcagc gcaatgcacc gcgctgccag ccgcttcgcg atcgaggccc agaacgtcgg
     6661 ggtgacgggc gacgtccgac ctgcattggc gaccctcggc gaggtcatga gcgcgtgcgt
     6721 cggctgtcat gccggctatc gcttcaagta ggccgacagc ggcagctccc gtgctacgca
     6781 cccgggctac gcaagtgtcg ggaacagatc gcgatagccc atggccgagc ggtcgaagag
     6841 gatgtcgcgc ggcgtcatgc ggcgggcgag tgcgaggccc gcaagcgtgc ggcaacgcga
     6901 aagcgccaca taggtctggc cgtgcgcaaa ggtgccgcgg ccgagatcga tatagacctt
     6961 gtcgagcgtc agcccctggc tcttgtggat ggtcagcgcc caggcgaggc gcagcggcag
     7021 ctgacggaac gtcccggcga tcgtctcgac gatcttccct tgcgcctggt cgaaggcata
     7081 gcgccgcgcc tcccaggcga cctgctcgac ttcgtgctcg ctccctttga tctcgacgaa
     7141 gacccgcttc tcctcgagcc gcgagatgcg cgcgatggtg ccgttcaccc agcgccggtc
     7201 gctgtcgttg cgcagcagga tgaccttggc accaggcttc aggacgagtg tcgcgtcggt
     7261 cgggtgcgcg ctctcagagt agtcgccggt gacgcccgcc tcgtagctca tcgctttgcc
     7321 cggcagtgca tcgaggaaag cgagattgat gcggttcgcc gcggcatttg tcggcgtcag
     7381 gatcacgtaa gggtcgccct cggagagcgt gcggatcgga tgcacacgct cgttcagggc
     7441 ctcgagatcg gcctcgtccg cgtcgccgtc gcggatgcga ttgaggacgc ggagcaacgc
     7501 ctcgtcacgc tggcggaaga cctgcgtcag ctcgaggagc gcggtgccgg cgccttcacg
     7561 cagcgtcgac acggagaaga agaacggccc gccgtaggtc gcctcgagat gggccgcgac
     7621 ctccgcctcc tgcacgaccg gcggcagctg gtggagatcg ccgaagagaa tgaggcgcac
     7681 gccgccaaag ggctcgcgcg gccgcccgcg attgagccgc agcgactggt cgatcgccca
     7741 catcaggtcg gagcgcacca tcgacacctc gtcgatgatg agatgcttca ggcggcgcat
     7801 cacgctgccg ttacgactgc ggcggatgtc ctcgctccgg atgaggcgcg gcggcaggcc
     7861 gaagaacgag tggatcgtct gcccgccgac attgacggcc gcgagcccgg tcggcgcgac
     7921 gatggcacgc tcctcgggca ccatgtcgac gattgacctg agaagcgtgg atttgcccgt
     7981 gccggcgcgg cctgtgatga agaggttgcc cgcgccctcg cgcacatggg cgatcgccgc
     8041 cgcatactcc gatgtcggct cgacgccgct cggccatgtc tcgacactga gcgagggttg
     8101 cattgccgcc ccgctcgccg tcgtgacctg atcggccttt gccgccttca atcgccgctc
     8161 ccgatgagcc ttaccgacac caccgactca agacgtcgcc gccgaacacc ctgccagatc
     8221 tcggggctga ccgacagtgc agccgggacg cgaccactca ttcgtgctgg tccatgcgag
     8281 ggggaaggcc gcgcgcccgc tcgatccagc gggcgagccg gaccatggcg aaggcgatcg
     8341 cgccgaggcc cgcgagcacg gccgccatcg gaatgagcac gggcacgaag ttccagatga
     8401 accagcccgt caccacagca agcccgagga gcagccacgc cttcagcagc atagcggctc
     8461 aggtgccgac gacgagcggc gccgtcaggc ggcggagtgc tgcaatcgcc gacagcgccg
     8521 tcaccttgcc cgtggccggg ttctcggcgg aggggatgtt ctcgatagtc atggtgaggt
     8581 cggagctgtc ggacttgacg cggatcgtat gcgtgttgcg cgtcaccgcc ggatcggccc
     8641 acacctctac acgcgtgcgc tccggcccga tgccggcgag cgaaagggcg accgccacgt
     8701 tcacattcgc cgggaagccc ttgatggcct cgcgggcatt gccctcgaac accttcttgg
     8761 cggccgtgag cccctcgacg gagatggcgt tctcgatgag gtgcggcgcg ccaatgaggc
     8821 ctttcggcgg cttacgcgtc gagatcgtga cactctcgac gttcccttcc gcaaccgcgc
     8881 gcacggcatc gaggccgagc agcgcgccgg tcggcacgat gatgcgcgcg cccgtcgtct
     8941 ttgccttgtc gacgagatgc atgttgccaa gcagctggcc gacggagagc ggcatgaaga
     9001 tgcggccgcg cgcgaccgcc gcctcggcga cctcggcgaa gtgccgcgct ggaatgcatt
     9061 cgaggatgac ctcggcccgc tcggcaagct cgggcagggt cacgttctcc gggaaagcgc
     9121 ggaaactggc gaggttgcgc tcggccttcg cgcgatccct ggcagcgaca gcgacgagct
     9181 tcagccctgg aacagttccc tgatcgacct tccgcgcaac cgcgaggccg atggcaccga
     9241 gcccggcgat ggccagcgtg agcgtattct gcgtcatccc cgctccaaca cgtctcgatg
     9301 accgcactcg ggcggccgat ggagagacga ttgcggcgat cgggcggaga ggtcaagcgt
     9361 cgggcaccga gcaccgcaat cggccgagcc cgatgatcac ccttgtgatc gcaccacttc
     9421 acggcccatg atgtcacgaa cacaaatgcg cgggacgaga ccagccatgg cccagacgaa
     9481 cctagcaaag ctcaatgccg ccgaagtggt ggtgaagacg ctgatcgcca acggtatcaa
     9541 agagctttac tgccttcccg gcgtgcagaa cgacgacttc ttcgatgcgc tcgcccgcat
     9601 cggtggcgcc atccggccga tccacacgcg ccacgagcag gcgactgcct acatggcgct
     9661 cggcgcggcg ctggcgacag gcaagccgca ggcgtactgc gttgtccccg gcatcggctt
     9721 tctcaatgcc tcgacgccac tcgccaccgc ccaatcgctg acccaacccg tgctctgcat
     9781 ctcgggccac atcgtcgagc gctacatcgg caagggctac ggctatctcc acgagatccc
     9841 gaaccagctc ggcatcatga agacgctcac caagtgggcc gagcacatcg gcggcgcgga
     9901 ggatgccggg ccgaagatcc gcgaggcctt ccgacagctg aactcgggcg tgccgggtcc
     9961 cgtcggcatc tcgtgcggct gggaccggtg gaagcatgcg ggcgaggtcg ccttcgacga
    10021 cgaggcagcg acgcgcagga cgccggcgat cgacctcgac caggtgaagg ctgcggcaaa
    10081 gctgctcggc aaggcgaaga agccgctgat cgtggtcgga ggcggcgcgc agggcgcaag
    10141 tgcggaggtg acggcactcg ccaacatgct ggaagcacct gttgccgcca atcgcaccgg
    10201 gcaaggcgtc ctcgacagcc gccaccacct cgcgattcgc aacgtctatg cgcaccggat
    10261 ctggaaggac gtcgacgtcg tgcttgcgat cggcacgcgg ctccagcagc accagatcga
    10321 ctggggcctc gacgacgaca tcaaggtgat ccatctcgat atcgatccca cccgcatcgg
    10381 acgcatcacc aagcccgaag tcggcatcat cgcggacgcc gcggagggtg tgcgggcgct
    10441 catcgacgcg cttgccaagc acaacatgaa gcgcgcgagc cgcaaggagg agatggcgga
    10501 gctcaaggcc cgcgtcggca aggagctcga ggtgctcgcc ccccagaagg cctgggtgga
    10561 cgccatccgc cgcacggttc ccgaggacgg catcgtcgtc gaggagctga cccaaatcag
    10621 ctatgcgagc cgcttcctct atcccgtgta caagccgcgc acctatatct cgagcggcta
    10681 tcagggcacg ctcggctggg gcctctcgac cgcgctcggc gtgaaggtcg cgatgcccga
    10741 tcgcgcggtg ctctcgatca acggcgacgg cggcttcatg ttcggcgtgc aggaattggc
    10801 gacggccgtc cgtcacaaca tcccgctcgt cagcgtcgtc ttcaccgatg acgcctacgg
    10861 caacgtgcgg cgcatgcagg cggcgaacta cgaaggccgc gtcatcgcga gcgacttgaa
    10921 aaaccccgat ttcgtgaaac tcggcgaaag cttcggcgtg cgctcagagc gcgccaagac
    10981 gccggacgcc ctcgccaagg cactcggcac ggccttcaaa gcgaaggagc cgagcctcat
    11041 cgaggtgccg gtcggcgagt tccctgatcc ctggaagctc gtcttcctcg gccgcgtcag
    11101 ggggacgaag cctggctgag gtggcgcgag acgagtggcg agagctgtac gaggaggccg
    11161 gctgagacga tgagcgcggc gccgagccag ccgacgagcc cgagccgctc gttcaggaac
    11221 atgtagccgg cgatggctgc aaagagcgtc tcgaggctga cgatcacggc ggcctcggcg
    11281 gccggcgtcg cgcgcatcgc ataagtgaaa gccgtgaagg tgatcgcacc tgcgagtacg
    11341 ccgagataga ggatctgcgg cgtggcggcc atcaccccgg caaacgcgac cgtctcgagc
    11401 gcgagtgcgc ccgtggccgc cagcgcgaac acggtcgcga actggacgca catgagcgtc
    11461 agcggccggg cgagcgccgt cggccggctc agtgacacca catggccggc ccaaaagaac
    11521 gcgcagacga tgatccagag atcgccctgg ctgaaatgac cgagcgtgcc gccgccaaga
    11581 agccacgtcc cggcaaacga catggcggct gcgggccaca cgacgctcgc cggcagcttg
    11641 ccgagcgtca cccaggcgat gaacggcacg aggattacgt agagcgccgt caagaagccc
    11701 gcgttcgtga ccgtcgccgt ggtgaggccc agctgctgga acgcggcgcc tgaaaagaaa
    11761 aggagcccgc agaaggcacc atggcaaagg atgtcgcgat cgagcgcgag ccccttgcgg
    11821 cggagctctg aataggcgag cggagcaagc acgacggctg ccagcgccgc gcgcgaggcg
    11881 acgaaggtca gcggcccgac gtgctccatc gccgtcttct ggaacaggaa ggcaaagccc
    11941 cagatcacgg cacagccgag caggatgaga tcagcacgga agcgggtcat cgatcctgat
    12001 ggcgcttctg ggcgcgcttg tgggcgacga gggcggcgag gcgctcgtcc cgccactggc
    12061 tcttgcggcg gatggcctcg cgcggcggcg ccgggcccca gctctctgcc gccttcttcc
    12121 agctcgcatc atcccatacg ctcgccggcg gcatgtcctt catgtagcgg cggaaccact
    12181 cgacatagcg cgtgcagtag tcggcgatgc cctcgggcgc gttgagctcg atcgtctcga
    12241 aaggccccat gaaggcccag cgcaggccga ggccgtcggc gatggtgtga tcgaggtccc
    12301 tcggcgagac atagccctcc tggaccaacc ggaaggcctc ggtgaggagg gccacctgca
    12361 gccggttgag gatgaagccg tcgacctcct tcttcaccgt gatcggcacc tgccccgcgc
    12421 tctggtagat ggcgcgggca cgggcgacga catccggact cgtccagggc gcaccgcaga
    12481 gctcgacgat cggcacgacg tgcggcgggt tcannnnnnn nnngggatgg gcgatcaggc
    12541 agcggctccg tccggcgagc ccttcggtaa acttcgaggc gacgatcgcc gacgacgacg
    12601 aggcgaggat cgcagtcgcc ggcgcgaggc gatcgagctc ggcgaagatc gcgagcttgg
    12661 cgtcgaggcg ctccggcccg ttctcctgca cgatctcggc gccggcgacc gcttcgctca
    12721 tcgaggtgca ggtggtgatc cgctttgcgg cgccggccgg gtcggcgacg aggccgtgcc
    12781 gggcaagctc gtcgagcgag gccgcgatca ggtcccgcgc ctcgcgcagc gtcgcctcat
    12841 gcggatccca gaggcggacg tcgaagccgc tccgcgcgaa cacgttgnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnggttgcc atggggcctc cctcgcttgg ccgccgacca
    12961 taggggcaag ccgcccgcag aaccagcggc ttcgccgtca gtcccgtgac aggaggcttg
    13021 cccacgtggt gacgaggcga gcttttcccc accggcaaca gcgtgcttga ccggtgcgtc
    13081 cgcggcgggt ttggttccgc ctgcaacttg ggattcgcga ggttcggagg tcgccatggc
    13141 tgagacaggc actgaggggc ggacggcgct cgaccatgcc aagcgcgcct atcgaacggg
    13201 ctcgttcttc tggcgggcgt acaactggct tggcttctcg agcatcagca ccggcaacgc
    13261 cgtcgccggc ctcacggcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacga
    13321 cgcggtcgac caccagatcc gggcgaaggc gatgccgcca ccggtcgtca tgacccgatg
    13381 ggacgacaac tcggtcgtct tcccgatcac cggcttcgac aaggccggca aacgggcgag
    13441 cttcaacgtg ctgctgctga cgaaggagta tgcctgggtg cgcggcagca cgggcgaact
    13501 cgtcaagggc gggcgggtct tggcagctgg cgacgttctg aaggagatct tcagcgagcg
    13561 cgtacggcag ggcctgaagc cgtcgaagca gctgatcgcc gtcggtatcg cctcgcagga
    13621 aggtgacccg gatgtcgagc tgaaacgtgc cggcgaccgc gcgaagcaga ccgccgattg
    13681 gacacgcgca gcgctcggcg cggaggtgga actgtggacg ctgaacctcg gccagtaccg
    13741 gatcgcctgc accggctgcg agaccggtga cacgaactgg cagcgcccgt tcgtgctgat
    13801 tgcggtgcgt gaccaggaag cagccgtcgt gatcgccgag gcgctcgcca atgccatgac
    13861 cgaccgcgcg aacgtgcctt cgcccgagag ctactcggcg ttcagaatgg agcggctgaa
    13921 atagtccaga accgatgtgg gcccggctgg tgccagcccg tcttcgagtc agcagtccaa
    13981 ttcacaaaga gaaacggcag atgtcgacag tgatcactat cctcggcgtc gcggcgctcg
    14041 cgatcgccgg cattctcatg gtcgcctcgc ggcgccccga tacgttccgc gtcgagcgct
    14101 cggctcaagt tgcagcgccg atcgcaaacg tctacccgcg catcgcgagc ctccgcgcga
    14161 tgaacgagtg gaacccgttc gtctcgccgg acccggacat caagatcacc tacagcggcc
    14221 ccgacctcgg ccgcggcgcc gcgcacacct gggacggcaa ccgccacgtc ggcgcgggcc
    14281 atatcgaggt gatcgaggcc gaggcacccg caaaggtcgt catgcggctc gtcatggtga
    14341 agccgattgc cgctgacaac cgggtggaat tcacactcga gccgagcggc gacggcacaa
    14401 aggtgacctg ggccatgagc ggccgccagc cgctgctcgc caagtgcatg acgctcttct
    14461 acgactgcga tcgcatggtg ggcgggcgtt tcgaggcggg cctcgcgggc ttgaagcgca
    14521 tagcggaggg cgggtcacgc gcgagctgag agaccgttgc ttcaggtctt gaagggctct
    14581 gctccgcgcc ccgtcaatgg cagtcatcgc ggagcgatgc tcgcatcgat ggccttcgga
    14641 accctttcgc gccgctcggt cggtgaattc aggaccgctt ggtcgcccgc atcgcctcct
    14701 cgagcgcctg caggaagcga gagcggtcgg ccttcgtgaa gctcgcgttg tagcccttgc
    14761 tctccccggt ttcacgc
//
LOCUS       DAORNH010000082        14676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3106_length_14676_cov_2.776759, whole genome shotgun sequence.
ACCESSION   DAORNH010000082 DAORNH010000000
VERSION     DAORNH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14676
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3106_length_14676_cov_2.776759"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..528
                     /locus_tag="PK264_10265"
     CDS             76..528
                     /locus_tag="PK264_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76310.1"
                     /translation="MKLLGEGMYWQDMKVGDSFRTFGRTITEPDIVAFIGVTGMLEVL
                     FTNEVYRQTESAIKGRLAPGALVFSFAEGLTIAGTIQGTGLAFLGMQLDIKAPSFAGD
                     TIHVEIEVVEARATSKGGRGIVRTRNVVVNQRGETVMIYTPTRMMKGR"
     gene            complement(941..2173)
                     /locus_tag="PK264_10270"
     CDS             complement(941..2173)
                     /locus_tag="PK264_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76311.1"
                     /translation="MPVSSAAIQSFLVRRGTVAPEPIRHGLDVQSRPSRFRSPRALRN
                     LRLGLTQLGVGPEIIAFSYCWRADGILLYAVEYDGEPDLWIRDLDRTRIADHIAQKIL
                     PFGYLPSRGYAAIQARERGLWALGTIAHIPSRSENLRSADLHRRLNDLKISIDRQAPL
                     IDLLTAFATSDKALCEVRRLPRDDKANETDVGSVGTSRDKHFALLHLRGRMGTRKNEL
                     ERVLDVVNGRERSSHYLNVPWLPICIVAACIALASVLDYMLPENDPGLVSDSLGSWAA
                     QQWSLLGSSRFRSFLIGGLTLGAIGHFYFWYLNFYRKRLALLTSGVVYFNNTVTYSAT
                     VNALYAKYARDLETSLAGAARPTDFPELATVTRSFEGVIDELKARVQHEQMKCRDRSI
                     GYAVVAAAAAFVGKLFVG"
     gene            complement(2252..2557)
                     /locus_tag="PK264_10275"
     CDS             complement(2252..2557)
                     /locus_tag="PK264_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76312.1"
                     /translation="MRHGDIVKYSLPVQLQGDADLKRKLQSRQAVILDQLPERVRREI
                     FRHAVLGYFRVKHHGGLTVVYDNRRLLDASRWGFSKSIRLKEILELNNVEIAEPALT"
     gene            complement(2660..3019)
                     /gene="rpsP"
                     /locus_tag="PK264_10280"
     CDS             complement(2660..3019)
                     /gene="rpsP"
                     /locus_tag="PK264_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRD76313.1"
                     /translation="MALKIRLSRGGAKKRPYYYIVIANSASPRDGRYIEQIGTHNPIL
                     PKDHADRVKLDLDRAKYWLGVGAQPTDRVARFLDAAGLMKREASNNPEKSKPKKKRQE
                     REAAAAKAAEGGEAKSA"
     gene            complement(3067..3579)
                     /locus_tag="PK264_10285"
     CDS             complement(3067..3579)
                     /locus_tag="PK264_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD76314.1"
                     /translation="MSLGLETERLRLRPWRESDLPAYAEFCASDAAEFVGGRCSAEDA
                     WRRIATFLGHWALRGFGPWIMVDKSSGRPAGYTGLWMPSGWPEGEILWSLFDGFRGRG
                     FATEAATHVRRHAYEELGWATAASFIHPKNAASLAVAERLGCAREGAIDLRGATIEIW
                     RHPPPSRLLN"
     gene            complement(3583..5211)
                     /gene="ffh"
                     /locus_tag="PK264_10290"
     CDS             complement(3583..5211)
                     /gene="ffh"
                     /locus_tag="PK264_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRD76315.1"
                     /translation="MFQSLSERLSGVLDRLTKRGALTDSDVAEAMREVRRALLEADVA
                     LDVVRDFTEKVKAKAVGQEVIKSVTPGQMVVKIVHDELVSMLGSTAEPIDLAAPAPIP
                     IMLVGLQGSGKTTTCAKISKRLTDRDRKRVLMASLDTRRPAAQEQLRVLGTPTGVDTL
                     AIVSGQTPQQIARRAMDAARLGGYDVVMLDTAGRIHIDEELMLESAEIRDIAEPHETI
                     LVADALTGQDAVNLAKSFNERIGITGIVLTRVDGDGRGGAALSMRAVTGKPIKLVGVG
                     EKWDALEEFHPSRIASRILGMGDVVSLVEKAAQTIDVEKAQKIAQKMRKGELDLEDLA
                     EQLRQMLRIGGMSGMMGMLPGVGKLKAQMADANLDDKVVKRQIAIIQSMTPRERRNPK
                     LLDGKRKRRVASGSGTRPEDVNKLLKMHRQMADMMKAMGKNPGMMRRMAGALGMGGPM
                     GMGAAPSAAEIERMQTELARLDPKALEQLPKEVRDQLAAAAPKAPPAALPISGAKPSA
                     EPPKLGGLPGLPGLGGRFKLPGLPGLPGLPAKKK"
     gene            complement(5211..5855)
                     /locus_tag="PK264_10295"
     CDS             complement(5211..5855)
                     /locus_tag="PK264_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD76316.1"
                     /translation="MLKVWGRKTSINVQKVMWTVGELGLKHERVDLGGPFGGLDTPGY
                     GKLNPNRLVPTIEDDGVIVWESNAIVRHLAQKYGRGSLSPSDEATYARADSWMDWTLT
                     SLYADVISTCFLGLIRTPASERNAPAVEAAARRAADKLALLNAQLADRPFIMGDQLTI
                     ADIPVGSLMYRYFNLAIPRPKMPHVEAWYARLMARPAFQTHVMIEFNSMKVPGA"
     gene            6343..6675
                     /locus_tag="PK264_10300"
     CDS             6343..6675
                     /locus_tag="PK264_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76317.1"
                     /translation="MRSISCFIVGAALGLVSSAALAFQENKATPAPGSTPPGAATVAP
                     GAAAQPSGPAGTEIRIPGLGRIGTLPRLELGLDLLYGVGDAPATPGRPEDRTDDGVQI
                     HGRIKHRF"
     gene            6815..7525
                     /locus_tag="PK264_10305"
     CDS             6815..7525
                     /locus_tag="PK264_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine synthase"
                     /protein_id="HRD76318.1"
                     /translation="MNGRIAAAAAVHVFTALGIVCALMAIRAVWSGTYEAAFAWLGLA
                     FIIDGIDGTFARAAKVTTYLPRFSGERLDMVIDYITYVLIPVLALLQAGFLAGTLGLL
                     LGAAILMSSLYHFADHGNKSDDNCFVGFPAIWNIVAFYFFAFGAKPWLVALIVIVCVA
                     LTFVPMRWVHPMRVKRLWLPTALACVAWSVAAAWTVWRGFPAEVWAKVVLLLVLAYGV
                     GLSLWWPWSNPNDQVHEG"
     gene            7547..8440
                     /locus_tag="PK264_10310"
     CDS             7547..8440
                     /locus_tag="PK264_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD76319.1"
                     /translation="MRWFGGLATLLAVALGGPATAEQVVHEHAGLELVGNLVVPAGRS
                     LEKDGAVLILHGTLAFHGQETVASLQKALGAKGKPSLAITLSLGLNARKSMFDCAIEQ
                     DHRNVDAVEELVAWTAWLKGRGVKRITVAGHSRGAAQVALFARDVLDPAIGAVVLIAP
                     VAQSRATVTTRYRAAFKADLDGVLAEARRLVEAGDDDKLMDVAFLTCPRTRVTAAAFL
                     DYYDDERTLELAKLLPELKLPVHVVLAEKDEVAAETPAALDRLGRLAHVTRATIPGAD
                     HFFRDLSSDDLADAVVSAAAR"
     gene            8595..8993
                     /locus_tag="PK264_10315"
     CDS             8595..8993
                     /locus_tag="PK264_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76320.1"
                     /translation="MRYKSLTACCAVAAFAGVAISGAAVHAEPSILTGDAIKATISGR
                     TVNMHTPVGTIPVVYRANGTMIGIAKDMSLYTGQERDKGTWWISGDKLCQRWQAWLDG
                     RQHCVSIKMTGQTFHWVSSDGRKGSATISH"
     gene            complement(9003..9812)
                     /locus_tag="PK264_10320"
     CDS             complement(9003..9812)
                     /locus_tag="PK264_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD76321.1"
                     /translation="MAADEPQFLTVGDGVAERRIAYLAQDASTPGRPGLVWLSGFRSE
                     MTSTKATALAGYGREHGLAVTRFDYSGHGQSGGTFEEGTVSRWLDEADAVFSGATAGP
                     QVLVGSSMGGYIALLLLLRDSARSAERRRVAGLVLIAPAWDMTERLMWARFPESVRRE
                     IETTGVYNRPSAYGDGPYAITRGLIEDGRRHLIADTPFNPGCPVRILHGLLDPDVPWE
                     HGLDLEALLLGDWTRVTAVPDGEHRLSRPEDLELLFELVGEVVDLATPSRA"
     gene            9866..>10999
                     /locus_tag="PK264_10325"
     CDS             9866..>10999
                     /locus_tag="PK264_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD76322.1"
                     /translation="MSAKRTTILQIIPELDTGGAEISTIEIAGGIVAAGGRAIVLSEG
                     GRLAPRLAEVGAELMLFPAATKNPVKLFANARSIARLVNSEGVHLLHARSRAPAWSAL
                     LASRRAGVPFVATYHGAYAETNALKRAYNSVMVRGDAVIANSEYTGRLIRERYGVGGD
                     RLIVIHRGVDPSRFDPAKITPERIAALRARFDVEADQPVIVNAARLTGWKGQSVLIAA
                     AGELLKSGRLGRAVIVLAGDAQGRDGYRERLETDIAALGLREHVRLAGHVEDIPAAFA
                     LAHLCVIASTEAEAFGRTATEAQAMGVPVIATAIGAPPETVLAGAPDMRTGWHVPPGD
                     APALAAAIGEALALSSEARRAMGARGIARVRAHFTLDRMRLDTL"
     assembly_gap    11000..11096
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11108..12019)
                     /locus_tag="PK264_10330"
     CDS             complement(11108..12019)
                     /locus_tag="PK264_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76323.1"
                     /translation="MRAKLRRMLWKYWTYRWQVSVDGEVYDVRHEVGWTSNVLTVSRG
                     GAQLASEALNYHREPFRLQEVAVDTPGGPLTFQTAPRTWLSYGLRVLRGETIVHQSHA
                     DPFALLPTMLKVTGFGTSEVGKAQVAASKQYYPAMAVDFAMGVILYFAAGFMSLRDVA
                     ILGAALIFAVQFAEWILDRLLGRKTNLTGGLSVLGVFFLLLSAGFAWLVDNDLAIQLK
                     SSILGLISAAVLAVDAIYDGRYIGVRIARFFTFIEIDPRRFSGLSALVAAGQSTLSAA
                     IALFLSKNAWLFYRHWIGPALGLALGL"
     gene            12177..13004
                     /locus_tag="PK264_10335"
     CDS             12177..13004
                     /locus_tag="PK264_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRD76324.1"
                     /translation="MLPEYEIHAIRYATRDALRRDHFIGGDPHELPMAMDYYVWVAIG
                     GAGAYVIDTGFTAEMAKARKRTYLRCPIDSLKLVGVEAGDVKDVILTHMHYDHVGNFD
                     RFAGARFHLQAREMAYATGKYMRYPRIGHSYHVEDVVGMVRLNFKGRVEMHEGEVEVA
                     PGIILTPTPGHSDGLQVVRVHTRRGWVVLASDATHFYENMWTNRPFTTAFHIGQMLDA
                     YRTLERLAPSPRHIIPGHDPHVMREYPASTPGLEGIAVRLDRDPTGAPYRLPGTAGH"
     gene            complement(13024..14229)
                     /locus_tag="PK264_10340"
     CDS             complement(13024..14229)
                     /locus_tag="PK264_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD76325.1"
                     /translation="MVALLSAAIAGGCASLVPRNGLPEGAIVEKAVVADNPGVRYFAD
                     HVPHDLAEEMRRRLPSLTRLSTSGTTADGRPIVDILALSGGGSDGAFGAGLLAGWSAR
                     GDRPKFDVVTGVSAGAIIAPFAFLGSGYDKELREIWTQYETSELVVSKIVPGLLGGPA
                     LSDTAPLRALIEKYVDRRLLSGIAREYGRGRILLIGTTNLDAQRPVVWNMGEIALRAS
                     RGDKSAAALFRDVILASAAIPGAFPPVSVKVEVDGKSYEEMHVDGGVMREVFVSPVEV
                     PYAAFDRFYGSAKKPLRRIFVVKNGKLVPEYTVVPAKAVSIAARSIATLIKAQHRADI
                     YRIYRLSRDDSAEFRLAAVPPAFNVKAKEAFDPAYLEALFEEGFKFGRIGTGWMRAPP
                     ELAVTRGPH"
     gene            complement(14456..>14676)
                     /locus_tag="PK264_10345"
     CDS             complement(14456..>14676)
                     /locus_tag="PK264_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRD76326.1"
                     /translation="ICGVTDDGKAGLAVGVTDDLTKAWSAVDLVKVGAEALGGKGGGG
                     RPDMAQAGGPDGSKAEAAVAAIEAKLAG"
BASE COUNT         2504 a         4623 c         4926 g         2526 t
ORIGIN      
        1 aaggcgttct tcgaaggcct gcgcacggac gtggtcgcag cgaacatcgg atcggaacag
       61 aaagggagtg ggcggatgaa attgctcggc gagggcatgt actggcagga catgaaggtc
      121 ggcgacagct tccgcacgtt cggacgcacg atcacggagc ccgacatcgt cgccttcatc
      181 ggagtgacgg ggatgctcga ggtgctgttc accaacgagg tctaccggca gaccgagagc
      241 gcgatcaagg ggcgcctcgc gccgggcgcg ctcgtcttct cattcgcgga gggattgacg
      301 atcgccggca cgatccaggg gacgggcctc gccttcctcg gtatgcagct cgacatcaag
      361 gcgccgtcgt tcgcgggcga cacgattcat gtcgagatcg aggtcgtcga ggcgcgtgcc
      421 acgtcgaaag gaggacgcgg gatcgtccga acaaggaatg tggttgtcaa tcagcgcggc
      481 gagacagtca tgatctacac gccgacgcgc atgatgaagg gacgctgagg cgccggcgag
      541 gacggggagg gatcgtcagt gcgccgatga cggcgagaaa tgaggagatc gagcaacact
      601 ttggggtcaa tgcacgactt ttcccagtta agatcggtga tcaggatcaa cattcgctga
      661 cgactcccgc atagttttct tgttcgaatg attcgaacac tttggaggca gaaggagacg
      721 acaatggcta aggacgcagc gaagaagccg gcagcgaaga agcctgcagc ggcagcgaag
      781 aagcctgcag cagccgcgaa gaagccggcc gccaagaaga agtgatgcgt cggtcctgag
      841 cgcggccagc agctcagcga ccccatacct cctgtaagtt tcgagccgag gagccaagct
      901 cctcggcttc gttgtggtgt ggcggcatcg cgacggtcgc ctatccgacg aacaatttgc
      961 cgacgaacgc ggctgccgcg gcgaccacag cgtagccgat cgaacgatcc cgacacttca
     1021 tctgctcgtg ctggacgcgt gccttcagct catcgatgac gccttcgaag gatcgagtaa
     1081 cggtcgccag ctctgggaag tccgtcggcc gtgcggcacc agccagcgaa gtttcaaggt
     1141 cacgagcgta cttggcgtag agcgcgttga ccgtcgcgct gtaggtgacg gtgttgttga
     1201 agtagacgac gccgctcgtg aggagtgcca ggcgctttcg atagaaattc aggtaccaga
     1261 agtagaagtg gccgatcgcg ccgagggtga gcccgccgat cagaaagctc cggaaccggc
     1321 tgcttccgag cagggaccac tgctgcgctg cccagcttcc aaggctgtcg gaaaccaggc
     1381 cagggtcatt ctccggcagc atgtagtcca agacgctcgc cagtgcgatg caggctgcaa
     1441 caatacaaat cggaagccaa ggcacgttca ggtagtgcga cgatcgctcg cgaccgttga
     1501 cgacatcgag gacacgttcc aactcgttct tgcgggtgcc catgcgccct cggaggtgaa
     1561 gcagagcaaa atgcttgtcg cgcgaggttc caaccgagcc aacatcggtt tcattggcct
     1621 tgtcgtcgcg agggaggcga cgcacctcgc agagtgcctt atcggaggtg gcgaaggcgg
     1681 tcagcaggtc gatcagcggc gcttgccgat cgatgctgat cttgaggtcg ttgaggcgcc
     1741 tgtggaggtc ggcgctgcgc aggttctccg agcgggaggg gatgtgagcg atggtgccga
     1801 gagcccataa gcctcgttcg cgcgcctgta tggcggcata acctctcgaa gggagatagc
     1861 cgaaagggag aatcttttgc gcgatgtggt cggcgatgcg cgtgcgatcc aagtcgcgga
     1921 tccacaagtc cggctcgcca tcgtattcca ccgcgtagag caggatccca tcagcacgcc
     1981 agcaatagga aaaggcaatg atctcgggtc cgacgccgag ttgggtgagg cccaatctca
     2041 gattgcgcag tgctcgcggc gatcgaaaac gggagggacg gctctgcaca tcaagaccgt
     2101 gccttatcgg ttcgggcgcg acggttccgc gcctcaccag aaatgattgg attgcggcgc
     2161 tcgagaccgg catccagtcg aggtagaaat actcgtgacg agccatggac gaacatccac
     2221 acgcaaaacg cgaccaaaga ctgcttggcg atcaggtcag cgccggctct gcgatctcga
     2281 cattgttgag ctctagaatc tctttcagcc ttatggactt ggagaaaccc cacctactgg
     2341 cgtccaggag ccttcggttg tcgtagacga ccgtgaggcc tccatgatgc ttgacgcgga
     2401 aatagccaag cacggcgtgg cggaaaattt cgcgccggac cctttccggc agctggtcga
     2461 ggatcacggc ctgtctggac tgcagctttc tcttcagatc cgcgtcgcct tggagctgga
     2521 ccggtagact gtatttcaca atgtcaccgt ggcgcatgct tgtctccatc ggggctgacg
     2581 ccacttggac tacgcctgct gcacgagcgt gcatcgagtc gcggtccaag tggtcgcagt
     2641 tccgccacgt ggttccgaat tacgccgact tcgcctcgcc accctcggcg gcctttgcgg
     2701 cggcggcctc gcgctcctgg cgcttcttct tcggctttga cttctcgggg ttgttggagg
     2761 cctcgcgctt catgaggccg gcggcatcga ggaagcgggc aacgcggtcg gtcggctgcg
     2821 cgccgacgcc gagccaatac ttggcgcgat cgaggtcgag cttcacgcgg tcggcatgat
     2881 ccttgggcag gatcggattg tgcgtgccga tctgctcgat gtagcggccg tcgcggggcg
     2941 aggccgagtt ggcgatgacg atgtagtagt aggggcgctt cttggcgccg ccgcgggaga
     3001 ggcgaatctt caaggccatg taagtctcca aggttcttgg ttgggtatgt ctgttcacgt
     3061 cttacgctag ttcagaaggc ggctcggcgg cgggtgccgc cagatctcga tcgtggcgcc
     3121 gcggagatcg atggcgcctt cgcgggcgca gccgaggcgc tcggcgacgg cgagggacgc
     3181 ggcgttcttc gggtggatga agctcgcagc cgttgcccag ccgagctcct cgtaggcgtg
     3241 gcggcggaca tgggtggccg cctcggttgc aaagccgcgc ccgcggaagc cgtcgaagag
     3301 cgaccagagg atctcgcctt caggccagcc ggacggcatc cacagccccg tgtagccggc
     3361 cggccgtccg ctcgacttgt cgaccatgat ccagggaccg aagccccgca gcgcccagtg
     3421 gccgagaaag gtcgcgatgc gccgccaggc gtcctcggcc gagcagcgcc cgccgacgaa
     3481 ctcggcagcg tcggacgcgc agaactcggc gtaggctgga aggtcgctct ccctccatgg
     3541 cctgaggcgc agccgctctg tctcgagacc gagggacatg actcacttct tcttcgcagg
     3601 caggccgggg aggcctggaa ggcccggcag cttgaagcga ccgccgaggc ctgggaggcc
     3661 cgggagcccg ccgagcttcg gcggttcggc cgagggcttt gctcccgaga tcggcagcgc
     3721 ggccggcggc gccttcggcg cggcggcggc gagctgatcg cgcacctcct tcggcagctg
     3781 ttcgagcgcc ttcggatcga ggcgggcgag ctccgtctgc atacgctcga tctcggcggc
     3841 gctgggcgcc gctcccatgc ccatcggtcc gcccatgccg agcgcgccgg ccatgcggcg
     3901 catcatgccg gggttcttgc ccatggcctt catcatgtcg gccatctggc gatgcatctt
     3961 gaggagcttg ttgacgtcct cgggtcgcgt gccggagccc gaggcgacgc gccgcttgcg
     4021 cttcccgtcg agcagcttcg gattgcggcg ctcacgcggc gtcatcgact ggatgatggc
     4081 gatctggcgc ttgacgacct tgtcgtcgag attggcgtcg gccatctgcg ccttgagctt
     4141 gccgacgccc ggcagcatgc ccatcatccc ggacatgccg ccgatgcgca gcatctggcg
     4201 gagctgctcg gcgaggtctt cgaggtcgag ctcgcccttg cgcatcttct gcgcgatctt
     4261 ctgggccttc tcgacatcga ttgtctgcgc ggccttctcg acgaggctca cgacatcgcc
     4321 cattccgagg atgcgcgagg cgatgcgcga gggatggaac tcctcgagcg cgtcccactt
     4381 ctcgccgacg ccgacgagct tgatgggctt ccccgtcacc gcgcgcatgg agagtgcggc
     4441 gccgccgcgg ccgtcgccgt cgacgcgggt gagcacgatc cccgtgatgc cgatgcgctc
     4501 gttgaaggac ttggcgaggt tcactgcgtc ctggcccgtc agcgcgtcgg caaccaggat
     4561 cgtctcgtgc ggctcggcga tgtcgcggat ctcggcggat tcgagcatca gctcttcgtc
     4621 gatgtggatg cggccggccg tgtcgagcat cacgacgtcg tagccgccga ggcgcgctgc
     4681 atccatggcg cggcgcgcga tctgctgcgg cgtctggccg gaaacaatcg cgagcgtgtc
     4741 gacacccgtc ggcgtgccga gcacgcgcag ctgctcctga gctgccggcc ggcgtgtatc
     4801 gagcgaggcc atcaggacgc gcttcctgtc gcggtccgtg aggcgcttcg agatcttggc
     4861 gcaggtcgtc gtcttgccgg agccctgcag gccgaccagc atgatcggga tcggcgccgg
     4921 tgcggcgaga tcgatcggct cggcggtcga gccgagcatg ctgacgagct cgtcgtggac
     4981 gatcttgacg accatctggc ccggcgtgac cgacttgatc acctcctggc cgacggcctt
     5041 ggccttcacc ttctcggtga agtcgcggac gacatccagc gcgacatcgg cttcgagcag
     5101 cgcccggcgg acctcgcgca tcgcctcggc gacgtcgctg tcggtcagcg cgccacgttt
     5161 ggtgaggcgg tcgaggacgc cggaaagccg ctcggagagg gactggaaca tcaggcgccc
     5221 ggaaccttca tggagttgaa ctcgatcatc acgtgcgtct ggaaggcggg ccgcgccatc
     5281 agtcgcgcat accaggcctc gacatgcggc atcttcggcc gcgggatggc gaggttgaag
     5341 tagcggtaca tcagcgagcc gacagggatg tcggcgatcg tcagctggtc gcccatgatg
     5401 aacggccggt cggcgagctg ggcgttgagg agcgcgagct tgtctgccgc ccgacgggcg
     5461 gcggcctcga cggccggcgc attgcgctcg ctcgcgggtg tgcggatgag gccgagaaag
     5521 caggtcgaga tgacgtcggc gtagagcgag gtcagcgtcc agtccatcca gctgtcggcg
     5581 cgggcatagg tcgcctcgtc cgagggcgag agtgagccgc ggccgtattt ttgcgccaga
     5641 tggcgcacga tcgcgttgct ttcccagacg atcacgccgt catcctcgat ggtcggcacg
     5701 agacgattag ggttgagctt tccgtagccc ggcgtatcga gcccgccgaa cgggccgccg
     5761 agatcgacgc gctcatgctt caagccgagc tcgccgaccg tccacatgac cttctggaca
     5821 ttgatcgagg tcttgcgacc ccacactttc aacatggctc cactccctcg ccacccaccc
     5881 caccgcaggc gaacctagcg cagcgttgcg aggcgcgcca atggcccggg cgccatgatc
     5941 gggcgaaata aatgctcacc gcgccgtcgc cgacgcctca cggtcgaaga tgtccaaaca
     6001 ccggcggcgt ccgtgagcga aattcgccga cggacgttgg ccaccgacct ccgagcgccg
     6061 gcagccccga tgtgggctgg cgtcgccgtg tgtcggttgg tgtcgtggag tgacgacctg
     6121 ccgagaagtt cgccgtcgat acgcggaaag gcagaagctg tcaaacgcct tgccgccgcg
     6181 atgtgctcgc caggatgtag ctgatcagcc acatggccaa tcgacaacag ttccgtcggg
     6241 aaacgttcag cactccttaa ctgcgttgga aacatgcttg tcaggataga gagtcgcagc
     6301 caatgtaacg ccaaaccgta cgactgcggg gtcgtgaaag tcatgagatc gatcagctgt
     6361 ttcatagttg gtgcagcgct ggggctcgta agctctgcag cgctggcgtt ccaggagaac
     6421 aaggccacgc cggcgccggg aagcactccg ccgggcgcgg cgacggtggc gcctggtgct
     6481 gcggcacagc cgtcggggcc agccggcacc gagattcgca ttccgggcct cggccgcatc
     6541 ggcacactgc ctcgactgga gctcggcctc gatctcctct atggcgtggg tgacgcgccg
     6601 gcaacgcctg gccgcccgga ggaccgcacg gatgacggcg tgcagattca tggccgcatc
     6661 aagcataggt tctgatgccg aggctggtgc agaccgagca tacatgaaat atgaagggag
     6721 cgccgagcgc tccctttgac gcttctgggg ctcggttgcg gcaagcccgc gggcgcatgt
     6781 cgggcggcaa catctggcga gagttcagcg gacgatgaac gggcggatag cggcggcagc
     6841 agcggtgcat gtcttcactg cgctcggcat agtgtgcgcc ctcatggcca ttcgcgcggt
     6901 ctggtcgggc acctatgagg ccgcgttcgc ctggctcgga ctggcattca tcatcgatgg
     6961 catcgacggt actttcgccc gtgccgcgaa agtgacgaca tatctcccgc gcttttccgg
     7021 tgagcggctc gacatggtga tcgactacat cacttatgtc ctgatcccgg tgctcgcgct
     7081 gctgcaggcc ggcttcctcg cgggtacact cgggctcctg ctcggcgccg ccatcctgat
     7141 gtcgtcgctc tatcatttcg ccgatcacgg taataagtcc gacgacaatt gctttgtagg
     7201 ttttccagcc atttggaaca tcgtagcatt ctatttcttc gccttcgggg cgaagccctg
     7261 gctcgtcgcg ctgatcgtca tcgtctgcgt ggcgctgacg ttcgtgccga tgcggtgggt
     7321 gcatccgatg cgggtgaagc gcctctggct gccgacggcg ctggcgtgcg ttgcctggtc
     7381 tgtcgcggct gcctggaccg tgtggcgggg ctttccggca gaggtctggg caaaggtcgt
     7441 gctgctgttg gtgctcgcct acggtgtcgg gttgtcgctc tggtggccgt ggtcgaaccc
     7501 caatgaccag gtgcatgagg gataggctcg atcgatgatg cagtgtttgc gttggtttgg
     7561 cggtcttgca actcttttag cggttgctct cggtggtccg gctactgcgg aacaggtcgt
     7621 ccatgagcat gccggtctcg agcttgttgg caacctcgtc gtgccggccg gacgctcgct
     7681 cgagaaggac ggcgcggttc tgatcctgca cggtactctt gcgtttcacg gacaggagac
     7741 ggttgcctcg ctgcagaagg cgctcggggc caaaggcaag ccgagcctcg ccatcacgct
     7801 gagcctcggc cttaacgccc gcaagagtat gttcgactgc gccatcgagc aggatcatcg
     7861 caacgtggat gccgtcgagg agctcgttgc ctggacggcc tggctcaagg gtcggggagt
     7921 gaagcggatc accgttgccg gccattcgcg gggcgcggcc caggttgccc tgtttgcgcg
     7981 ggacgttctg gacccggcca tcggtgcggt tgtgctgatc gcgccggtag cccagtcgcg
     8041 agcgaccgtc acgacgcgct atagggccgc gttcaaggcc gacctcgacg gcgtgctcgc
     8101 cgaggcgagg cggctcgtcg aggccggcga tgacgataag ctgatggacg tagcctttct
     8161 cacctgcccg cgaacgcggg tcacggcggc ggccttcctc gactactacg acgacgagcg
     8221 cacgctggag ctggcgaagc tcttgcccga gctcaagctt cccgtgcatg tggtgctggc
     8281 tgaaaaggac gaggtcgcag ccgagacgcc ggcagcgctc gatcgcctcg gtcggctggc
     8341 gcacgtcacg cgtgcgacaa tacccggcgc cgaccatttt ttccgcgacc tctcgagcga
     8401 cgatctcgct gacgctgttg tgtctgccgc cgccaggtga tcgcgcgtta acgatcgcgg
     8461 cgatatgctg cattgcacaa tagccgatgc tctatgctag ccagcggggg tcgaggtcaa
     8521 attatcgtca tgttcggcat taacaagtct taacacagaa gaagccggac agaactgtca
     8581 gacggggaaa agtcatgcgt tacaagagcc tcacggcctg ctgtgccgtg gcagccttcg
     8641 caggcgttgc tatttcggga gccgccgtcc acgccgagcc gtcgattctc acaggcgatg
     8701 ccatcaaggc gacgatcagc ggccgcaccg tgaacatgca cacgccggtc gggacgatcc
     8761 ctgtcgtcta tcgtgcgaac ggtacgatga tcggcattgc caaggacatg tcgctctaca
     8821 ccggccagga gcgcgacaag ggcacctggt ggatcagcgg cgacaagctc tgccagcgct
     8881 ggcaggcttg gctcgacggg cgccagcact gcgtcagcat caagatgacc gggcagacct
     8941 tccactgggt gagctcggac ggccgcaagg gctcggcgac catctcgcac tgagcaccgt
     9001 tctcacgccc gggacggcgt cgcgaggtcg acgacctcgc cgaccaattc gaagagcagc
     9061 tccagatcct caggccgcga caggcggtgc tcgccgtcag ggaccgccgt gacccgcgtc
     9121 cagtcgccga gcagcaacgc ctcgaggtca aggccgtgct cccaaggcac gtccggatcg
     9181 aggaggccgt gcaagatgcg cacggggcag cctggattga acggcgtatc ggcgatgaga
     9241 tggcgcctgc cgtcctcgat gagtccccgt gtgatggcgt aggggccgtc accgtaggcc
     9301 gagggtcgat tgtagacgcc ggttgtctcg atttcgcgcc tgacactctc gggaaagcgc
     9361 gcccacatca gccgctccgt catgtcccag gccggtgcga tgagtacgag cccggcaacg
     9421 cggcgccgct cggccgatcg ggcactgtcg cgcagcagca ggaggagggc gatgtagccg
     9481 cccatgctcg agccaacgag cacttgcgga ccggctgtcg cgccggagaa cacggcgtcg
     9541 gcctcatcga gccagcggct gacggttccc tcctcgaacg ttccgcccga ttgaccatgc
     9601 cccgagtagt cgaagcgcgt gaccgcgagg ccatgctcgc ggccgtagcc ggcgagcgcc
     9661 gtcgccttgg tgctggtcat ctcggacctg aagccggaga gccagacgag tcctggtcgg
     9721 ccgggtgtac tggcatcctg cgccagatac gcgatgcggc gctcggccac accgtcgccg
     9781 acagtgagga attgcggctc gtctgctgcc accctaagcc tcccgaccac tgcagcgacg
     9841 cccatcactc gcggagccat cgcggatgtc tgccaagcgg acaaccatac tccagatcat
     9901 cccggagctc gatacgggcg gcgccgagat ctcgacgatc gagatcgcgg gcggcatcgt
     9961 tgccgcgggt gggcgggcga tcgtgctctc ggagggcggg aggctcgccc caaggctcgc
    10021 ggaggtcggc gccgagctga tgttgtttcc ggcagcaacg aagaacccgg tgaagctgtt
    10081 cgcgaatgcc cgatcgatcg cgcggctcgt caacagtgag ggtgtccacc tcctgcacgc
    10141 gcggagccgt gcgcctgcct ggagcgcgct gctggcgtcg cggcgcgcgg gcgtcccctt
    10201 tgttgcgacc tatcacggag cctacgccga gaccaacgcg ctgaagcggg cctacaactc
    10261 ggtgatggtg cgcggcgacg cggtgatcgc caactcggag tacacaggga ggctcatccg
    10321 cgagcgctac ggcgtcggcg gcgatcggct catcgtcatc catcgcggcg tcgacccatc
    10381 ccgcttcgat ccggcaaaga tcacgcccga gcggattgcc gcgctgcggg ctcggttcga
    10441 tgtagaggcg gaccagcccg tcatcgtcaa cgcagcgcgc ctcacaggtt ggaagggcca
    10501 atccgtgctg atcgcggcgg ccggcgaact cctcaagtcc ggccggctcg gccgcgctgt
    10561 gatcgtgctc gcgggtgatg cgcagggccg cgacggctac agggagcggc tcgagaccga
    10621 catcgcggcc ctcggcctac gcgagcacgt gcgcctcgcg gggcatgtcg aagacattcc
    10681 cgcagccttt gcgctcgcgc atctatgtgt catcgcgtcg accgaggccg aggccttcgg
    10741 acggacggca accgaggcgc aggccatggg cgtgcccgtg atcgcgacgg cgatcggcgc
    10801 gccgcccgag acggtcctcg ccggggcgcc tgatatgcgg accggctggc atgtgccgcc
    10861 gggtgatgcg ccggcgctgg ctgcggcgat cggcgaggcg ctggcgcttt cgtccgaggc
    10921 gcgccgggcg atgggcgcgc gcgggatcgc ccgcgtcagg gcgcatttca cgctcgaccg
    10981 catgcggctc gatacgctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgca
    11101 acagcaccta taggccgagc gcgagcccga gcgccggtcc gatccagtgc ctgtagaaga
    11161 gccaggcgtt cttggagagg aagagggcga ttgccgcgct gagcgtcgat tgacctgctg
    11221 ccacgagtgc cgaaagaccc gagaagcgcc tgggatcaat ctcgatgaag gtgaagaagc
    11281 gggcgatgcg cacgccgatg taccggccgt catagatggc atcgaccgcc agcacggccg
    11341 cgctgatgag cccgaggatg ctcgatttga gctggatagc gaggtcgtta tcgacgagcc
    11401 aggcaaagcc cgcggagagc agcaggaaaa agacgccaag cacggagagc ccgccggtga
    11461 ggttcgtctt tcgtccgagt aggcggtcga ggatccattc cgcaaactgg accgcgaaaa
    11521 tcagcgcagc cccgagtatc gcgacgtccc tcaggctcat gaagccggcc gcgaaataca
    11581 ggatcacgcc catagcgaaa tcgacggcca tggccggata gtactgcttg cttgccgcaa
    11641 cctgcgcctt gccgacctcc gatgtcccga agcccgtcac cttcagcatg gtcggcagca
    11701 gcgcgaaggg atcggcgtgc gactgatgga caattgtctc gccccgcagg acgcgtaggc
    11761 cgtaggagag ccacgtacgc ggcgcggtct ggaacgtgag cgggccgccg ggtgtgtcga
    11821 cggcgacctc ctgcagccgg aagggctcac gatggtagtt gagcgcctcg ctcgcgagct
    11881 gggctccgcc ccgcgacacc gttaagacgt tactcgtcca gccgacctcg tggcggacgt
    11941 cgtagacctc gccgtccacg ctgacttgcc agcggtacgt ccagtacttc cagagcatcc
    12001 tgcgaagctt ggcacgcatg accggcgatc ctcaaggctc gtgttgtgat aggacggctc
    12061 gattgcacgg cgatgggtct cgctggctgc gattcacgcg agggcaatag agcgtagtct
    12121 acacgattgt ctgcggctac cgtcgccttg agcattgtat tgatctggag gctgccgtgc
    12181 tgcccgaata cgaaatccac gccatccgct atgcaacccg cgatgccctc cgccgcgacc
    12241 atttcatcgg cggcgacccg cacgagctgc cgatggcgat ggattactat gtctgggtcg
    12301 cgatcggcgg ggcgggggcc tatgtcatcg acacgggctt cacggccgag atggcgaagg
    12361 cgcgaaagcg cacctatctg cgctgcccga tcgacagcct gaagctcgtc ggtgtcgagg
    12421 cgggcgacgt caaggatgtg atcctcaccc acatgcacta cgaccatgtc ggcaatttcg
    12481 acaggttcgc gggcgcgcgc ttccacctcc aggcgcgcga gatggcgtac gcgacgggca
    12541 agtacatgcg ctatccgcgc atcggccaca gctatcacgt cgaggacgtg gtcggcatgg
    12601 tgcggttgaa cttcaagggc cgcgtcgaga tgcacgaggg cgaggtcgag gtggcgccgg
    12661 gcatcatcct gacgccgacg cccggccact cggacgggct ccaggtggtg cgcgtccaca
    12721 cgcgccgcgg ctgggtggtg ctcgcgtccg acgcgacgca cttctacgag aacatgtgga
    12781 ccaaccgccc cttcacgacg gccttccaca tcggccagat gctcgacgcc taccgcacac
    12841 tcgagcggct ggcgccgagc ccgcgccaca tcatcccggg ccatgacccg cacgtgatga
    12901 gggagtatcc ggcctcgacg ccgggcctcg aagggatcgc cgtccgactc gaccgcgatc
    12961 cgacgggggc tccctaccga ctgccgggca cagctggtca ctgatcgagc cggtgttccg
    13021 gcgtcagtgc ggcccgcggg tgacggcgag ttccggcggc gctctcatcc agcccgtgcc
    13081 gatcctgcca aacttgaagc cctcttcgaa gagcgcctcg aggtaggccg gatcgaaggc
    13141 ttccttggcc ttcacattga aggcgggtgg aacggccgca aggcggaact cggcgctgtc
    13201 gtcgcgcgac agtcggtaga tgcggtagat gtccgctcga tgctgcgctt tgatcagcgt
    13261 cgcgatcgag cgcgccgcga tcgagacggc cttcgccggc acgaccgtgt attccgggac
    13321 gagcttgccg ttcttgacga cgaaaatgcg ccggagcggc ttcttcgccg atccatagaa
    13381 gcggtcgaag gcggcgtacg gcacctcgac cggcgagacg aacacctctc gcatcacgcc
    13441 gccgtcgacg tgcatctcct cgtaggattt gccgtcgacc tcgaccttga cggagaccgg
    13501 tgggaaggca cccgggatcg cagcggaggc gaggatcacg tcgcggaaca gcgccgccgc
    13561 cgatttgtcg ccgcggcttg cccggagcgc gatctcgccc atgttccaca cgacggggcg
    13621 ctgggcgtcg agattggtcg tgccgatcag caggatgcgg ccgcggccgt attcgcgcgc
    13681 aatccccgat agcaaccgcc ggtcgacata cttctcgatg agcgcacgca ggggcgcggt
    13741 atcgctgagc gccgggccac cgagcagtcc cggcacgatc ttggagacca cgagctctga
    13801 cgtctcgtac tgcgtccaga tctcgcgcag ctccttgtcg taaccggacc cgaggaacgc
    13861 gaacggcgcg atgatcgcgc cggcgctgac accggtcacg acatcgaact tcggccggtc
    13921 gccgcgcgcc gaccagccgg cgaggaggcc cgcgccaaag gcgccatccg agccgccgcc
    13981 ggagagcgcc aggatatcga cgatcgggcg gccatcggcc gtcgtgcccg acgtcgacag
    14041 ccgtgtgagg ctcgggaggc gccggcgcat ctcctcggcg agatcatggg ggacatggtc
    14101 ggcgaagtag cgaacgccgg ggttgtcggc gacaaccgcc ttctcgacga ttgcgccctc
    14161 cgggaggccg ttgcggggaa cgaggctcgc gcagccgccc gcgatcgccg cgctgagaag
    14221 cgcgaccatg ccgagcgccg ttcgggagct gatcgcggcg agggccttca taggattgca
    14281 agttgctccg gacagggcaa tggagaatgc cagatcgcgc tccgagacgg tgagtagcag
    14341 tcagaagagc ttatagctca gttccatctg acgacctgtg gctggccagc aacgaatccc
    14401 gtaccatgag ccgatacggc tcgtcggtag ctgggcgctc cgacgtccag acggctcaac
    14461 ccgcgagctt cgcctcgatg gcggcaacag cagcctccgc cttgctgcca tccgggccgc
    14521 ctgcctgcgc catgtcgggc cggccgccgc cacccttgcc gccgagcgcc tctgcgccga
    14581 ccttgacgag atcgacggcg ctccacgcct tggtgagatc gtccgtgacg ccgacggcaa
    14641 gcccggcctt gccgtcgtcg gtgacgccgc agatca
//
LOCUS       DAORNH010000083        14623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3120_length_14623_cov_2.866625, whole genome shotgun sequence.
ACCESSION   DAORNH010000083 DAORNH010000000
VERSION     DAORNH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14623
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_14623_cov_2.866625"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PK264_10350"
     CDS             complement(<1..732)
                     /locus_tag="PK264_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD76327.1"
                     /translation="MIWKPELDELRRREAFVREMGGADKVRRQHDGGRLTVRERIDKL
                     ADAGSFHEIGAIAGKAAYDADGQLKNLTPSNCVMGRAKVEGRPVIICGDDFTVRGGSA
                     DATISQKAIMMENMAHDMRLPIIRVIEGSGGGGSVKTIETTGRSNLPGGINDNTPTYH
                     ICSVNMGVVPVVGLGLGSVAGLGAARLAAAHYSVMTKSSAMFVAGPPVVERIGQKLTK
                     QELGGWEIQCRAGGVDHAVETEEEAF"
     gene            782..1423
                     /locus_tag="PK264_10355"
     CDS             782..1423
                     /locus_tag="PK264_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HRD76328.1"
                     /translation="MSLSLMMWRRHYASFDARLAQRAAFEAEASDMPPPFRPMSKQRF
                     AAEVAGFSWQRRVWRVDVHHTFIPDHARFREIGGLGCQEGMCRHHVVERRFDDIAQHV
                     TIAPDGVIWSGRDWNKMPASVGFGLNRGAFMLEMIGNFDVGHDRLDGAQLETTFAVVA
                     AVQHHFKLPAEALLFHREVPVTEKTCPGTGVEKRTFLAGLSEHRARLALLTSA"
     gene            complement(1410..2195)
                     /locus_tag="PK264_10360"
     CDS             complement(1410..2195)
                     /locus_tag="PK264_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRD76329.1"
                     /translation="MFIVNETRRRLEAGELALGIGMRQARTADFPQIAKTCGFDWLFI
                     DMEHSSYDVDDAALFCAAGLGVGITPIVRVPGFEHYHSSRVLDSGAQGIVAPHISTPE
                     EAKKIVSACRFPPIGKRSMTGTQPQARFATMSMPDIVAGANKETLVVCMIETPEGLAN
                     VDKIAATPGVDVLLIGASDMSTELGIAGQIGHAKIEDAVGKIVAACKSHKKYPGLGGV
                     YDPALMEKYIGMGMRFILSGSDVSFLMAGARARSEMLRKIKLK"
     gene            complement(2297..3076)
                     /locus_tag="PK264_10365"
     CDS             complement(2297..3076)
                     /locus_tag="PK264_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76330.1"
                     /translation="MLRRTFITGLVGVTLATATLGALPALASPNPSWVHLGTHRVSHH
                     LDRDIIQVGREEGRFERIFFRVHGNGLNVQDVRVIYANGTDEHLAVSEFIPEGGDSRQ
                     LDLRGWRRSIDRIELVYESSNRHRPAATVHVYGLKSASFEPDPMPAPAHDRGWEVIGT
                     ARAGHIVDHDTIDVGRSRGRFRALMLRSLDRDIQVYSVRVTFANGETQDLAYAGLLRA
                     GSNSGVIDMSGHRRGIERVDLVYRTRGLLRSRANVELLGLH"
     gene            3255..4148
                     /locus_tag="PK264_10370"
     CDS             3255..4148
                     /locus_tag="PK264_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD76331.1"
                     /translation="MTASSTSRRAKILVSTEELAGMLGDPSLRIFDCTVHLHASADNS
                     TYRAEPGRADYDAGHIPGSAFLDLQVDLCDSAKLPIKFVVPTADAFAAGAGRLGIGEG
                     TRVVLYDRTRNQWAARVWWMLRMFGFDDVRVLDGGMVKWAKEGRPVAREATRYPAARF
                     IARPRSGMIVSKEEVLTAVETRQPGLTVLNALTSEQHRGAGGVHYGRPGRIPGSACVA
                     ARAIIDPETQAYLPDEQLRAMFAAAGALEADRVIAYCGGGIAASSAALALTFLGQDNV
                     AIYTNSLQEWALDPNLPMEVG"
     gene            4537..5946
                     /locus_tag="PK264_10375"
     CDS             4537..5946
                     /locus_tag="PK264_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRD76332.1"
                     /translation="MVRRRLAFGPAGVATLTFLGEIAVITLTAIAACMAYHWLALGTI
                     GSVRSFALVGGLTGILFAIPFLSRNEYRVASFLDGRRGPARIVGAWTYAFLALAGLAF
                     LSKTTASFSRGAVLLFAMTGLIMVFIYEGVTGALIKRLLRSGRVAPRRLMLVGKDDDI
                     RRYSERRGLPDVAARVVAYVSLPTVDPDAAGMARLSSALDRAAEQARAVGVDDILLLG
                     ALDDAAVSRCIDIFHRLPVGIHRDAGPALDQASEVRIARVGAVAAVSLTEPPLSPFGA
                     VLKRLFDIVVASIGLVLLAPLFLAVAIMIRRESAGPAFFRQRRQGFNQREFRIFKFRT
                     MTTLDDGDVIRQAKAGDVRITSFGAKLRRWNIDELPQLINVLRGEMSIVGPRPHAIAH
                     DRIFEKQIDVYPRRLNVKPGITGWAQVNGYRGETDTDEKLRGRVEHDLYYIDRWSVWF
                     DVYIILLTIVSPKAYRNAL"
     gene            6011..7282
                     /locus_tag="PK264_10380"
     CDS             6011..7282
                     /locus_tag="PK264_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRD76333.1"
                     /translation="MMRRSVAITRALLGLAKAALVTLLAAQCLVGAPPRAMADAAFTA
                     FIEALWPDAQALGISRATFDAAFKGVTPDLALPDLALPGKTETRGQAEFVRPPRDYLA
                     SAFIARLADQGRKLSLQHAQVLAEIERRIGVERHVVLAIWGRETAYGGHKSQHYAIRV
                     LATQAYTGRRKEMFRIELLQALKMLDEKVITIEAMRSSWAGAMGLTQFMPSEYFALAY
                     DLDGDGRRDIWGSVGDALASAANQLKGKGWLTGQPWGIEVRLALGGADCSYEGPDRTQ
                     PIAAWAKLGVLPVSGRAFSASELQLPAYLMMPAGAYGPAFLVTENFLVIKRYNMSDLY
                     ALFVGNLSDRIAGGGEFRTAFRDIAQLPTRDIEEVQRLLASAGVPMAKIDGKIGSNTR
                     GEIGRFQRARQIKVDCWPTGELLAHLRARTK"
     gene            complement(7283..7657)
                     /locus_tag="PK264_10385"
     CDS             complement(7283..7657)
                     /locus_tag="PK264_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76334.1"
                     /translation="MREFVAIDTAPEPIRIKRTRIQTLLQIANIAITAVVLGAWTFIA
                     LFVVEPIGWATWAPVTRGWRPGLFEYPFTMLWGLPLCAVALAWLARRANFSGLNYALL
                     LLPLALLGMIFGWYYLAPSDWL"
     gene            7916..9199
                     /locus_tag="PK264_10390"
     CDS             7916..9199
                     /locus_tag="PK264_10390"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRD76335.1"
                     /translation="MNAPATRDFLAATMATLGREARAAASVVALASNEERNEALGAAA
                     ARIRATTAEILAANQRDVEAARQRGITAAFLDRLTLSPARIEAMAKGLEEIAALDNPV
                     GRVSAEWSRPNGLKISKVHVPLGVIGIIYESRPNVTADAGGLCLKSGNAAILRGGSES
                     FHSSRAIHAALVHGLEMAGLPAAAISLVPTTDREAVGLMLAGLDRAIDVIVPRGGKSL
                     VARVQAEARVPVFAHLEGICHTYVDRGADLDVAIPLVLNAKLLRTGICGATECLLVDK
                     GAHEDYLTPLVKALIDAGCEVRGDAATLAADPRAKAAAPDDYGREFLDKIIAVKVVDG
                     VDEAIAHIRRYGSEHTDCIVTECNGTAERFLKRVDSAIVLHNASTQFADGGEFGFGAE
                     IGISTGRMHARGPVGVEQLTTFKYIVRGTGQVRSG"
     gene            complement(9206..9883)
                     /locus_tag="PK264_10395"
     CDS             complement(9206..9883)
                     /locus_tag="PK264_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76336.1"
                     /translation="MVDPSEAMDSDYGRATIDHAVKIVNASGSAACRAERKLDDAAIS
                     AKLRELFIRSATISAHYDRIIMQTPEYAEAFDLAIGRGAFETWQAYLKMADLKPYYAL
                     SRPMRLRDMIEDVAFTISKALVIERIDLKEPLSVLASGNGAMLAKSIEVETAVLEAME
                     RWRKTHPALDLDKFDEIQLQVAMAKFKVIETPMRSPELKVWLTMGPHQFAPGAVTVFT
                     SLCVGPQ"
     gene            10157..11932
                     /gene="xsc"
                     /locus_tag="PK264_10400"
     CDS             10157..11932
                     /gene="xsc"
                     /locus_tag="PK264_10400"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HRD76337.1"
                     /translation="MKMTTEEAFVKVLQMHGIEHSFGIIGSAFMPISDLFPKAGITFW
                     DVAHECNGGFMADGFARSSGKMAMVIAQNGPGITNFVTAIKTAYWNHTPMLLVTPQAA
                     NRTIGQGGFQEVEQMALFKDMVCYQEEVRDPVRIAEVLNRVIVKSKRLSAPAQINVPR
                     DYWTQVVDISLPQVVEFERPAGGELAIAAAAKLLSDAKFPVILSGAGVVLAGAIPECA
                     ALAEKLDAPVCSNYQHNDSFPGSHRLACGPLGYNGSKAAMEIIAKADVVLALGTRLNP
                     FSTLPGYGIDYWPKGAKVIQVDINADRIGLTKPVAVGICGDAKLVAKQILGKLTATAG
                     NAGREERKALVHKTKFAWLQTLSGMDHEDDDPGTTWNARARKAHPERLSPRMAWRAIQ
                     AGLPKEAIISSDIGNNCAIGNAYPTFEQGRKYLAPGLFGPCGYGFPAIIGAKIACPDM
                     PVVGFAGDGAFGISMSEMSSIGRKEWPGITMVVFRNYQWGAEKRNTTLWYDDNFVGTE
                     LHPELSYAKVAVGCGLKGVVVKTQAELTAALKESCEAQKKGLTTFIEVILNQELGEPF
                     RRDAMKKPVPVAGIDPKDMRPQKGA"
     gene            12579..12797
                     /locus_tag="PK264_10405"
     CDS             12579..12797
                     /locus_tag="PK264_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76338.1"
                     /translation="MKILISAIAGALIAISGTASAKSAFDTLAETAPKHPIFAELDRT
                     APRANIFDTLNQSAPKHPIFIELDRNAP"
     gene            complement(12889..13428)
                     /locus_tag="PK264_10410"
     CDS             complement(12889..13428)
                     /locus_tag="PK264_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD76339.1"
                     /translation="MKRRNGALVVPGLGSPIWDLMDAGDSPEFAYSWGGMGLAVPAAL
                     GLALAQPSRRVLAMTGDGEIMMGIGSLAVVADQAPKNLAILVLDNESFSETGKQRGLT
                     GSHTDLITVAKGFGIRETRLVTEAGDVSTLADFLFKSDGPVLAVAKVAITSDPFKLPE
                     KDGAAIAKRFRVALGLDKP"
     gene            complement(13500..14021)
                     /locus_tag="PK264_10415"
     CDS             complement(13500..14021)
                     /locus_tag="PK264_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD76340.1"
                     /translation="MPERHWSEDVHREMKSRSIKTVCTIPDGGLTQLLKLVEADRDMR
                     LVTLSTEEEGMGIVTGQHLGGERAMIAMQSSGVGNCINALGFPSAYRAPCLMLVTMRG
                     QWGEFNPWQVQMGSAVKPVLEAVGVKCFPVERAEEVGETFAAAADIAFYSSLSAAVLV
                     SQRVIGAKGFGLK"
     gene            14182..>14623
                     /locus_tag="PK264_10420"
     CDS             14182..>14623
                     /locus_tag="PK264_10420"
                     /inference="COORDINATES: protein motif:HMM:NF017950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HRD76341.1"
                     /translation="MARSGKIAAAVTALLVVTLAQVGTRAQAPGPRVEIGVLTCTVAG
                     GPGLILGSQKDMGCIFKRSDGSQERYDGRLTRVGVDIGITTTTVISWGVLAPTNLPAG
                     ALSGTYTGVTAEATIGAGLGANVLLGGSSRSIALQPVSVQAQQGL"
BASE COUNT         2516 a         4813 c         4749 g         2545 t
ORIGIN      
        1 gaaggcctcc tcttccgtct cgacggcatg atcgacgccg cctgcgcggc attggatctc
       61 ccagccgccg agttcctgct tcgtgagctt ctgcccgatg cgctcgacga cgggcggtcc
      121 tgcgacgaac atcgccgaag acttggtcat caccgagtag tgcgccgcgg cgagccgtgc
      181 cgcaccgagg cccgcgaccg agccgaggcc gagcccgacc acgggcacca cgcccatgtt
      241 taccgagcag atgtgatagg tcggtgtgtt gtcgttgatg ccgcccggca ggttggagcg
      301 ccccgtcgtc tcgatcgtct tcaccgagcc gccgccgccc gagccctcga tcactcggat
      361 gatgggaagg cgcatgtcgt gcgccatgtt ctccatcatg atcgccttct gcgagatggt
      421 cgcatcggcc gatccgccgc gcacggtgaa atcatcgccg cagatgatca ccggccggcc
      481 ctccaccttg gcgcgaccca tcacgcagtt cgaaggcgtg aggttcttga gttgtccgtc
      541 ggcatcgtac gcggccttgc cggcgatggc gccgatctcg tggaaggagc cggcatcggc
      601 gagcttgtcg atccgctcgc gcaccgtgag gcgtccgccg tcatgctggc ggcgcacctt
      661 gtcagcgccg cccatctcgc gcacaaaggc ctcgcggcgg cgcaactcgt cgagttcggg
      721 cttccagatc atcggcgtcc cttcacggca gcatggttct ctgcgagtat cggaagaacc
      781 gatgtcgctg tcactcatga tgtggaggcg gcactatgcg agtttcgatg cgcgccttgc
      841 acagcgcgca gctttcgagg cagaggcgag tgacatgccc ccgccattcc ggccgatgtc
      901 aaagcaaagg tttgccgcgg aggtagcggg cttctcatgg cagcgccgcg tgtggcgcgt
      961 cgacgtccac cacaccttca ttcccgatca cgctcgtttc cgcgagatcg gcggcctcgg
     1021 ctgccaagag ggcatgtgcc gccaccatgt cgtcgaacgg cgcttcgacg acatcgccca
     1081 gcacgtgacg atcgcgcccg acggtgtgat ctggagcggg cgggactgga acaagatgcc
     1141 ggcaagcgtc ggcttcggcc tgaaccgcgg cgccttcatg ctcgagatga tcggcaattt
     1201 cgatgttggc cacgatcgcc tggacggcgc tcagctcgag acaacatttg ccgtagtcgc
     1261 cgccgtgcag catcacttca agctgccggc tgaggcacta ctcttccatc gcgaggtgcc
     1321 ggtgacggag aagacctgtc ccggcaccgg cgtcgagaag cgcaccttcc tggcagggct
     1381 cagcgagcac cgcgccaggc tggccttgct cacttcagct taatcttgcg cagcatctcg
     1441 ctccgcgcgc gggcccccgc catcaggaag gagacgtcgg agcccgacag aatgaagcgc
     1501 atgcccatgc cgatgtactt ctccatcagc gccggatcat agacgccgcc gaggcccgga
     1561 tacttcttgt gcgacttgca ggcggcgacg atcttcccga ccgcatcctc gatcttggcg
     1621 tgaccgatct ggccggcgat gccgagctcg gtcgacatgt cgctcgcccc gatgaggagc
     1681 acgtcgacgc ctggcgtcgc ggcgatcttg tcgacgttgg cgaggccttc cggcgtctcg
     1741 atcatgcaga cgaccagcgt ctccttgttg gcgccagcga cgatatccgg catcgacatg
     1801 gtggcaaaac gcgcctgcgg ttgcgtgccc gtcatcgagc gcttgccgat cggcgggaag
     1861 cggcaggccg agacgatctt cttcgcttcc tcgggcgtcg agatgtgcgg ggcgacgatg
     1921 ccctgggcgc ccgaatcgag gacgcgtgag gagtggtagt gctcgaagcc cggcacgcgc
     1981 acgatcggtg tgatgccgac gccaagccct gccgcgcaga agagggccgc atcgtcgaca
     2041 tcgtaggagg agtgctccat gtcgatgaac agccagtcga agccgcaggt cttggcgatc
     2101 tgtgggaagt cggccgtgcg cgcctggcgc atgccgatgc cgagggcgag ctcgccggcc
     2161 tcgaggcggc gacgggtttc gttgacgatg aacatatggc ctccgagccg gatgggtgga
     2221 ggcccgattt gccgtcagtt tcctccgcca cgcaagcgcc gcctcgccta ttggcgtagc
     2281 gacgccgcat gggagatcag tggaggccga gcagctcgac gttggcccgg ctgcgcagga
     2341 ggccgcgcgt ccggtagacg agatcgaccc gctcgatgcc gcggcgatga ccggacatat
     2401 cgatcacgcc cgagttcgat ccggcgcgca agagcccggc ataggcgagg tcctgtgtct
     2461 cgccgttcgc gaacgtgacg cggacgctgt agacctggat gtcgcgatcg agcgagcgca
     2521 gcatgagggc acggaagcgg ccgcgcgacc ggccgacgtc gatcgtatcg tggtcgacga
     2581 tgtggccggc gcgtgccgtg ccgatcacct cccagccccg gtcatgggcg ggcgcgggca
     2641 tcggatcggg ttcgaaggag gccgacttca gcccgtagac atggaccgtc gccgcgggtc
     2701 gatggcgatt actcgattca tagacgagct cgatccgatc gatcgagcgg cgccagccgc
     2761 ggagatcgag ctggcgcgag tcgccgcctt ccggaatgaa ctccgagacc gcgagatgct
     2821 cgtccgtgcc attcgcatag atgacgcgca cgtcctggac attgaggccg ttgccgtgca
     2881 cgcggaagaa gatgcgctcg aagcggccct cttcgcgtcc tacctggatg atgtcgcggt
     2941 cgaggtggtg cgagacgcgg tgcgtgccga gatgcaccca gctcgggttg ggagaagcga
     3001 gcgccggcaa ggcgccgagc gtggcggtgg caagcgtgac tccaacgagc cctgtgatga
     3061 atgtgcggcg aagcatgggt ccgtctccaa tatcggcggc acgtcgcgcg ccgagaatga
     3121 ttggagattg gcggccggtg actgaacacg atctgagcgc gcgtttcaga ctgcattcat
     3181 cggaataact gttttccgtc acggtcggcg acggatatcc tgcctgccgg gttccttgcc
     3241 gggagaagcg tgccatgaca gcctcatcga catcgcgccg cgcgaaaatt ctcgtatcga
     3301 ccgaagagct tgccggtatg ctcggcgatc cctcgctgcg catcttcgat tgcacggtcc
     3361 acctccacgc cagtgcagac aactcgacct atcgcgccga gcccggccgc gccgactacg
     3421 acgccgggca catcccgggg agcgccttcc tcgacctcca ggtagacctc tgcgatagcg
     3481 ccaagctgcc gatcaagttc gtagtcccga ccgcggacgc tttcgcggcc ggcgccggac
     3541 gcctcggcat cggcgagggc acgcgtgtcg tgctttatga tcgcacacgc aaccagtggg
     3601 ccgcccgcgt gtggtggatg ctgcgcatgt tcggcttcga tgacgtgcgc gtcctcgacg
     3661 gcggcatggt gaaatgggcg aaggaaggcc gtcctgtcgc gcgcgaggca acgcgctatc
     3721 cggccgcgcg cttcatcgcg cgcccacgaa gcggcatgat cgtctccaag gaggaggttc
     3781 tcacggccgt cgagacgagg cagccggggc tcaccgtact caacgcgctt acaagcgagc
     3841 agcaccgcgg cgccggtggc gtgcactacg gccggcctgg gcgcattcca gggtcggcgt
     3901 gcgtggcggc gcgcgcgatc atcgatcccg agacgcaggc ctaccttccc gacgagcagc
     3961 tgagggccat gtttgccgcg gccggcgccc tcgaggccga tcgcgtgatc gcctattgcg
     4021 gcggcggaat agccgcctcc agtgccgcgc tggcgctcac cttcctcggt caggacaacg
     4081 ttgcgatcta cacgaactcg ctgcaggagt gggccctcga cccgaatctg ccgatggagg
     4141 ttggttgaga ggtgccgccc tgcaatccgg acaccggcgg cgcgtgctcc tgacgcacgc
     4201 gaaatggcgg aggcgtgctg aacaccctcg cgccgatcat catcttccgg aacaatgccc
     4261 attggagcac gctgcacccc cgccggcagg gcctttgccg cgtgcagcaa agtgaatttc
     4321 atttaggtat ggcggagact taagcacgtc agcagcaatt tgatgcgaat tcgcttgatt
     4381 ggggattaca agtcgttaca agcgtcaggc gtgcgggcac gctgcagtgc agcataggcc
     4441 cggtcggaag ctctgatccg accggcggcg cccgaaagat gtgggtagcg ggctggtcgg
     4501 atgagcggca ctcagcgtat tgcgttccag cgatccatgg ttcggaggcg acttgccttc
     4561 gggccggcag gcgtcgcaac actcactttc ctcggcgaaa tcgcggtcat cactctgacg
     4621 gcgatcgcgg cctgcatggc ctatcactgg ctcgccctcg gcaccatcgg ctcggtgcgc
     4681 agctttgcac tcgtcggcgg cctcaccggc atcctgttcg cgatcccctt cctgtcgcgc
     4741 aacgagtacc gtgtggcgag cttccttgat ggccgccgcg gcccagcgcg catcgttggt
     4801 gcctggacct acgctttcct cgcgctcgcc ggcctcgctt tcctgtcgaa gacgacggcg
     4861 agcttctctc gcggcgccgt actgctattc gccatgaccg ggctcatcat ggttttcatc
     4921 tacgagggcg tcaccggtgc gctgatcaag cgcctcttgc gctccgggcg cgtcgcgccg
     4981 cgacgcctca tgctggtcgg caaggatgac gacatccgcc gctactcgga gcgccgcggg
     5041 ttgcccgacg tcgccgcgcg cgtcgtcgca tatgtctccc tcccgacagt cgatccggac
     5101 gcggcgggca tggcccgctt gtcgagcgcg cttgatcgcg ccgccgagca ggcgcgcgcg
     5161 gtcggcgtcg atgacatcct cctgctcgga gcgctcgacg atgccgcggt cagccgctgc
     5221 atcgacatct tccatcgcct gcctgtcggc atccaccgcg atgccgggcc ggcactcgac
     5281 caggcgtccg aggtgcgcat cgcccgggtc ggcgccgtcg cggcggtgtc gctcaccgag
     5341 cccccgctga gcccgttcgg cgcggtgctc aagcggctgt tcgatatcgt cgtcgccagc
     5401 atcggccttg tattgctcgc gccgctgttc ctggccgtgg cgattatgat ccgccgcgag
     5461 agcgcgggcc ctgcattctt ccgccagcgc cgccagggct tcaatcagcg cgagttccgc
     5521 atcttcaagt tccgcacgat gaccactctc gacgacggcg atgtcatcag gcaggccaag
     5581 gctggcgacg tgcgcatcac gtctttcggc gccaagctcc ggcgctggaa catcgatgag
     5641 ctgccgcagc tgatcaacgt gctgcgcggc gagatgtcga tcgttggccc acgcccgcat
     5701 gcgatcgccc acgatcgaat cttcgagaag cagatcgacg tctacccgcg ccgcctgaac
     5761 gtgaagccag gcatcaccgg ctgggcgcag gtgaacggct atcgcggcga gactgacacc
     5821 gacgagaagc tgcgcggccg cgtcgagcac gacctctact acatcgatcg ctggtcagtc
     5881 tggttcgacg tctacatcat tctgctgacg atcgtttcgc ccaaggccta ccgaaacgcg
     5941 ctctgaccgt ccgctgctat cttgaaatcg cctggaaccc ggcgccttat ctcgccggga
     6001 ggatgcaggc atgatgcgac ggtcagtggc gatcacgcga gcattgctcg gattggcgaa
     6061 ggcggcgctc gtcacgcttc tcgccgcgca atgccttgtc ggcgccccgc cccgggccat
     6121 ggccgacgcg gccttcaccg ccttcatcga ggcactctgg cctgacgcac aggcgctcgg
     6181 catctcacgg gcgacgttcg acgccgcctt caagggcgtc acgcccgatc ttgccctgcc
     6241 cgatctggcg ctcccaggta agacggagac gcgcggccag gcggagttcg tgcgcccgcc
     6301 gcgtgactat ctcgcgtccg ccttcatcgc gcgtctcgcc gatcaggggc gcaagctctc
     6361 cctccagcac gcccaggtcc tcgccgagat cgagcgccgc atcggcgtcg agcgccacgt
     6421 cgtgctcgcc atctggggcc gcgagacggc gtacggcgga cacaaatcgc agcattacgc
     6481 gatccgcgtg ctcgcgacgc aggcctacac cggccggcgc aaggagatgt tccggatcga
     6541 actgctgcag gccctcaaga tgctcgacga gaaggtgatc accatcgagg cgatgcggtc
     6601 atcgtgggcc ggcgccatgg ggctcacgca gttcatgccg tcggagtatt tcgcgctcgc
     6661 ctacgatctt gacggcgacg ggcggcgcga catctggggc tcggtcgggg atgcgctcgc
     6721 ctccgccgcg aaccagctca agggcaaggg gtggctcacc ggccagcctt gggggatcga
     6781 ggtgcgtctg gcattaggtg gcgcggactg cagctacgag ggccccgatc gcacacagcc
     6841 cattgccgca tgggccaagc tcggcgtgct gccggtctcg ggacgcgcct tcagcgcgag
     6901 cgagttgcag ctgccggcct atctgatgat gcccgccggc gcctatgggc ccgcgttcct
     6961 ggtgacggag aactttctcg tcatcaagcg ctacaacatg tcggatctct acgccctttt
     7021 cgttggcaac ctgtcggacc ggatcgcagg cggcggcgag ttcaggacag cgttccgcga
     7081 cattgcacag ttgccgacgc gggacatcga ggaggtgcag cgcctgctcg cttcggcggg
     7141 ggtgccgatg gcgaagatcg atggcaagat cggctcgaac acgcgtggcg aaatcggcag
     7201 gttccagcgg gcgcggcaga tcaaggtcga ttgctggccc acaggggagc tactcgcgca
     7261 tctgcgcgcg aggacaaagt aatcaaagcc agtcggaggg tgccagatag taccagccga
     7321 agatcatgcc gaggagggcg agcggcagca gcagcagtgc gtagttgagg ccgctgaagt
     7381 ttgcgcgccg cgcgagccat gcaagagcga cggcgcagag cggcagtccc cagagcatcg
     7441 taaaggggta ctcgaagagc cccggccgcc agccgcgcgt gaccggagcc caggtggccc
     7501 agccgatcgg ctcgaccacg aacagtgcaa taaacgtcca ggcgccaagc acgactgccg
     7561 tgatcgcgat attggcaatc tgcaatagcg tctggatccg tgtccgcttg atgcggatcg
     7621 gctccggagc tgtgtcgatg gccacgaatt cgcgcacggc gagagaacct caagaatgct
     7681 tagacgtcga cgatactcaa aattgcttaa cgaagtgctg agacgggacc agccgttctc
     7741 gtgatgaacc agcacttggc acgcgtgaca gcgctgcttt ccagttccgg cgacattggc
     7801 aggcgtgcta gaagccgtac gacaagcgct tggcggcgcg tgcagagccg tggccatcgc
     7861 atttcaccgt catgcgttca ctgtaatggt tgcccgccag caaggatgag acgcgatgaa
     7921 tgctccagca acgcgcgact ttcttgccgc gactatggcg accctcggcc gcgaagcgcg
     7981 cgcagctgcc agcgtcgtgg cgctcgcatc gaacgaggag cgcaacgagg ctctcggcgc
     8041 cgcggcggcg cggatcaggg cgacgactgc tgaaatactc gccgccaatc agcgcgacgt
     8101 cgaggctgcc cgtcagcgcg gaatcaccgc cgcgttcctc gaccgcctca ccctcagccc
     8161 ggcgcgcatc gaggcgatgg cgaagggcct cgaggagatc gcggccctcg acaatccggt
     8221 gggccgcgtc agcgccgagt ggtccagacc gaacggcctc aagatcagca aggtgcatgt
     8281 gcccctcggc gtcataggca tcatctacga gagccgccca aacgtgacgg cggatgcggg
     8341 cggcctctgt ctcaagtcgg ggaatgcggc catcctgcgc ggtggctcgg aaagctttca
     8401 ttcctcgcgc gccatccatg cggccctcgt ccacggcctc gagatggccg ggttgcccgc
     8461 tgccgccatc tcgctcgtgc cgaccacgga tcgtgaggcc gtcggcctga tgctcgccgg
     8521 cctcgaccgc gcgatcgacg tgatcgtgcc gcgcggcggc aagagtctcg tcgcgcgcgt
     8581 tcaggcggag gcccgcgttc ccgtgttcgc gcatctcgag ggcatctgcc acacctatgt
     8641 cgaccgcggt gccgatctcg atgtggcgat ccccctcgtg ctcaacgcga agctgctccg
     8701 caccggaatc tgcggggcga ccgagtgcct gctcgtcgac aagggcgcgc acgaggatta
     8761 tctgacgccg ctcgtcaagg cgctcatcga tgccggctgc gaagtgaggg gcgacgcggc
     8821 gacactggcc gccgatccgc gcgccaaggc cgcggccccc gacgactacg gccgcgagtt
     8881 cctcgacaag atcatcgcgg tcaaggtcgt cgacggcgtc gacgaggcga tcgcccacat
     8941 ccgccgctac ggatccgagc acactgactg catcgtcacc gagtgcaacg gcacggccga
     9001 gcgattcctg aagcgggtcg acagtgccat cgtgctccac aacgcatcga ctcagttcgc
     9061 cgatggcggc gagttcggct tcggcgccga gatcggcatc tcgaccgggc gcatgcacgc
     9121 gcgcggcccc gtcggcgtcg agcagctcac gactttcaag tacatcgtgc gcggcaccgg
     9181 acaggtgcgc tccggttgat ggtgctcact gcggtccgac gcacaagctc gtgaacaccg
     9241 tcactgctcc aggcgcgaat tggtgtgggc ccatggtcaa ccacactttg agctcgggcg
     9301 agcgcatcgg ggtctcgatc actttgaact tcgccattgc gacctggagc tggatctcat
     9361 cgaacttgtc gaggtccaag gccgggtggg tcttccgcca gcgctccatg gcctccaaga
     9421 ccgcggtctc gacctcgatt gacttggcga gcatcgcacc gttgcccgag gcgagcacgc
     9481 tcaagggctc cttgagatcg atacgctcga tgacgagcgc cttcgaaatt gtgaacgcca
     9541 catcctcgat catgtcgcgc aggcgcatgg gcctgctcaa cgcgtagtac ggcttcaagt
     9601 cggccatttt cagataggct tgccaggtct cgaaggcccc gcgtcctatg gcgaggtcga
     9661 acgcctcggc gtactcgggc gtctgcatga tgatgcggtc atagtgagcg ctgatcgtgg
     9721 ctgaacggat gaacaattcg cgaagcttgg cggagattgc agcgtcgtcg agcttgcgct
     9781 cggcgcggca tgcggcactc ccgctcgcat tcacgatctt cacggcatga tcgatcgtgg
     9841 ctcggccata gtcgctgtcc atggcctcgg acggatcgac catttggagc tcgaccagat
     9901 ctgcaggcat ggccgcgtgc gacacttcgg gacgtacgat caagccgcac cagaccgcaa
     9961 aagcagtcgc gacaaatcga aaggcgaagg ctcgcatggc gaatccccag agccgaccac
    10021 gctcgaatgg tagcggcgcg gcggcccgct gcaaggccaa tccggtcatg cactccgatc
    10081 aggctgacgc acgcgcgtcg gcgtgcaata gtccgcccat cacacggcac aagaccaaga
    10141 cgcgaggaga cggctcatga agatgacgac cgaagaagcc ttcgtgaagg tgctgcagat
    10201 gcacggcatc gagcactcgt tcggcatcat cggctcggcc ttcatgccga tttccgatct
    10261 ctttcccaag gccggcatca ccttctggga cgtcgcccac gagtgcaacg gcggcttcat
    10321 ggccgacggc ttcgcgcgct cgtccggcaa gatggccatg gtgatcgctc agaacgggcc
    10381 cggcatcacc aacttcgtga ccgccatcaa gaccgcctac tggaaccaca cgccgatgct
    10441 gctcgtcacg ccgcaggccg cgaaccgcac catcggccag ggcggcttcc aggaagtgga
    10501 gcagatggcg ctcttcaagg acatggtctg ctaccaggag gaagtgcgcg atcccgtccg
    10561 catcgccgag gtccttaacc gcgtgatcgt gaagtcgaag cggctctcgg cgcccgcgca
    10621 gatcaatgtg ccgcgtgatt actggacgca agttgtcgac atcagcttgc cgcaggtcgt
    10681 cgagttcgag cggccggcgg gcggcgagct cgccatcgcg gctgcagcaa agctgctctc
    10741 ggacgccaag tttccggtga tcctgtccgg cgcgggcgtc gtgcttgcgg gcgcaattcc
    10801 ggagtgtgcg gccctcgccg agaagctcga cgcgcccgtc tgctcgaact accagcacaa
    10861 cgacagtttc cccggaagcc atcgcctcgc ctgcggcccg ctcggctaca acggctcgaa
    10921 ggctgccatg gagatcattg ccaaggccga cgtggtgctc gccctcggta cgcggctcaa
    10981 tccgttctcg accctccctg gctacggcat cgactactgg ccgaagggtg ccaaggtcat
    11041 ccaggtcgac atcaacgccg atcgcatcgg cctgacgaag cccgtcgccg tcggcatatg
    11101 tggcgatgcc aagctcgttg caaagcaaat cctcgggaag ctcacggcga ccgccggcaa
    11161 tgccggccgc gaggagcgca aggccctcgt ccacaagacc aagttcgcgt ggctccagac
    11221 gctctccggc atggatcacg aggacgacga tcctggcacg acctggaacg cgcgcgctcg
    11281 caaggcccac ccggagcgcc tgtcgccgcg catggcctgg cgcgccatcc aggccgggct
    11341 gccgaaagag gcgatcatat cctccgacat cggcaacaac tgcgccatcg gcaacgccta
    11401 cccgaccttc gagcagggtc ggaaatatct ggcgccgggc ctcttcggcc cctgcggcta
    11461 cggcttcccg gcgatcatcg gtgccaagat cgcctgcccg gatatgcccg tcgtcggctt
    11521 tgcgggtgac ggcgccttcg gcatctcgat gagcgagatg agctcgatcg gccgcaagga
    11581 gtggcccggc atcacgatgg tcgtgtttcg caactatcag tggggcgcgg aaaagcgcaa
    11641 caccacgctc tggtacgacg acaatttcgt cggcaccgag ctgcacccgg agctctcgta
    11701 cgccaaggtc gccgtcggct gcgggctgaa gggcgtcgtc gtgaagactc aggcggagct
    11761 gaccgcggcg ctcaaggaga gctgcgaggc gcagaagaag ggcttgacca ccttcatcga
    11821 agtgatcctc aaccaggagc tcggtgagcc attccggcgc gacgccatga agaagcccgt
    11881 cccggtcgcc ggcatcgacc cgaaggacat gcgcccgcag aagggggcat gaagtcgctt
    11941 ccatttgaca cgaaatcgac cgctgcgtcc ttgggcagag gtcggcggtc gtgtgctccg
    12001 cggtagttgg tcgcctccgg gacaggaggc gatcagtcca aagagtgcaa caaggccgtc
    12061 gtaatcgagg ctggatccct caagcatccc aatcccaata gttccggcgc aatctcgtgc
    12121 gatactgagc gccgacaagg gatggagtca tgactgcgcg tatccacagt gactgcggtg
    12181 tgattttcca agggcctcga tggaatttca gacgcggttc gcgaccgagc tggatcgccg
    12241 cacgcgtgat cgaaagcctc ctcgcccagc caccgttcaa aatgcgcaag ctgttcggcg
    12301 gcccgcgatg atgccgatgg ttcgggtcag ttcgcgcggc gctggcctga gcctacgggc
    12361 aggcatgccc catgttccca tcggatgcgg tcgagattgg gggtatttcg tttgtcactc
    12421 gttcagggga cgcacgcaac ttgattttgc aatagatttt tcagcggcgg atagtcgccg
    12481 atcgaattcg ggccgtcgat tggatggtcc ctcatttcac tgacgtgtcg attgtggcga
    12541 ccagcaccgc cgatactaca atccgaaagg accaagccat gaagatcttg atttccgcca
    12601 ttgcgggcgc cctcattgcg atctccggca ccgcgtccgc caagagtgcc ttcgatacgc
    12661 tcgccgagac ggcgccgaag cacccgatct tcgccgaact cgaccgcact gcgccccgcg
    12721 ccaatatttt cgacaccttg aaccagtcgg caccgaagca cccaattttc atcgagctcg
    12781 accgcaacgc gccgtgactg cactcggctg gccgcttttc ctcccacgta ttccggtcag
    12841 catcggggcc cggcacaccg ccgggcccca tttcgtagcc cagcgagttc agggcttgtc
    12901 gagtccgagt gcgacacgga agcgcttggc gatggcggcg ccatccttct cgggcaattt
    12961 gaacggatcg ctggtgatcg cgaccttggc gacggctagc accggcccgt ccgacttgaa
    13021 caggaagtcc gcgagggtac tcacgtcgcc ggcctcggtg acgagccgcg tctcgcggat
    13081 gccgaaacct ttggcgacgg tgatgaggtc ggtatgcgag cccgtgaggc cgcgctgctt
    13141 acctgtttcc gagaagctct cgttgtctag aacgaggatc gcgaggttct tcggcgcctg
    13201 gtcggcgaca accgcaagcg aaccgatgcc catcatgatt tcgccgtcac cggtcatggc
    13261 gaggacacgt cggctcggct gcgcgagtgc gaggccgagt gctgccggca ctgcgaggcc
    13321 catgccgccc caggaatagg cgaactcggg gctgtcgccg gcgtccatca ggtcccagat
    13381 cgggctgccg aggcccggca ccacgagcgc gccattgcgc cttttcatga gttcggccac
    13441 aaagggtcgg cggtggatct tattcggtgt tgcctgaggg gcgggtttcg ctgatttgct
    13501 catttgaggc cgaatccctt ggcgccgatg acgcgctggc tgacgagcac cgcggccgac
    13561 agcgagctgt agaaggcgat gtcggcggcg gcagcgaagg tctcgccgac ctcctcggcc
    13621 cgctccacgg ggaagcattt cacgccgacg gcttcgagca cgggcttcac cgcgctcccc
    13681 atctgcacct gccagggatt gaactcgccc cactggcctc gcatcgtgac gagcatcagg
    13741 cagggtgcgc gataggccga cggaaatccc aacgcgttga tgcagttgcc gacgcccgag
    13801 gattgcatgg cgatcatcgc gcgctccccg ccgagatgct ggcccgtgac gattcccatg
    13861 ccctcttcct cggtcgagag ggtgacgagg cgcatatcgc ggtcggcctc gacgagcttc
    13921 aggagctgcg tgagcccgcc gtcgggaatg gtgcagaccg tcttgatgct ccgcgatttc
    13981 atctcgcggt ggacgtcctc ggaccagtgg cgctcaggca tggggcttct ctctgatagt
    14041 gacgtgctcg tccgcgaaat gttcggccga ccgcatgcga gcgtcaaggc tcgcccgcac
    14101 tggaggcaag cgtgctcgcc atgctaaact cgcatgcgaa cgggcattgt cgcatcgccg
    14161 gcatggatgg aggtcgaagc catggcacga tcgggcaaaa tcgcagctgc cgtgacggcg
    14221 ctgctggtcg tcaccctcgc gcaggttggc acgcgcgctc aggcgcccgg cccgcgcgta
    14281 gagatcggcg tcctcacctg cacggtcgcc ggcgggccag gcctgatcct cggctcgcag
    14341 aaggacatgg gttgcatctt caagcgctcc gacggcagcc aggaacgcta cgacggtcgc
    14401 ctcacacgcg tcggtgtcga catcggcatc accacaacga cggtcatcag ctggggcgtg
    14461 ctggcgccga ccaatctgcc agcaggcgcg ctgtcgggca cctacacggg cgtgacggcc
    14521 gaggcgacga tcggagccgg gctcggggcg aatgtgctgc tcggcgggtc gagccgctcg
    14581 atcgcgctgc agccggtgag cgtgcaggcg cagcagggac tga
//
LOCUS       DAORNH010000084        14563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3131_length_14563_cov_2.694858, whole genome shotgun sequence.
ACCESSION   DAORNH010000084 DAORNH010000000
VERSION     DAORNH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3131_length_14563_cov_2.694858"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..963)
                     /locus_tag="PK264_10425"
     CDS             complement(28..963)
                     /locus_tag="PK264_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76342.1"
                     /translation="MKKLALGLIALVALLYGVALLRPKLTMAELGIDAADAARCEAPQ
                     LPVYVRPGYDRRVAANDYLPYAIAALNVYPDGRPKGFTLDKHSPEWRKIDTRTPSSSL
                     AMDVYHRDTPARLEVLVAFRGTQSLDASDWLANMSWALAWAPIATRYDEARAEFREVR
                     KAAFKTAGTKKVAFIAAGHSLGGGLAQHIAYAYPCVSAAIFNSSFVTNQFRLAEPNTK
                     AQIIHVFEDLDELTRLRRLLFIDKESETYKHYRQDAVNDRVEFQHSMVGLGVGMARQV
                     LRCQEKRAECPVPASETRVRSLYCSSWGKGGPLCK"
     gene            complement(1033..>1757)
                     /locus_tag="PK264_10430"
     CDS             complement(1033..>1757)
                     /locus_tag="PK264_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HRD76343.1"
                     /translation="ESLVERIPPPAGAVRETLAPGSFGAWLRGLPLKPKGAPVLLFDG
                     RAKPRQDVHIAVIDIDVGTRDLQQCADAIMRLRAEWLLASGRVGEIAFNFTGGGRVSF
                     ARWADGWRPRAERGGISWSQSAKADATYPSFRRYMDRIFSYAGTASLERELKPVAEPS
                     VGDVIIKGGFPGHAVLIVDAAILPSGARRFLLLQSFMPAQEMHILTNPSAADGSPWYD
                     LARGSAIVTPEWTFPAGALRRF"
     assembly_gap    1758..1807
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2247..2534
                     /locus_tag="PK264_10435"
     CDS             2247..2534
                     /locus_tag="PK264_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76344.1"
                     /translation="MTTLSTTVATASHGVAADANFEGKQHVGRPILTRIGDGFVGLYR
                     LLTAGQEARANRFVRPHLARQDDATLKTLGLSDAEIAKLRHEAADMPRIWL"
     gene            2620..2841
                     /locus_tag="PK264_10440"
     CDS             2620..2841
                     /locus_tag="PK264_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76345.1"
                     /translation="MKTTTIDHAFPSLSATTEASSAVRRPILEKLRAYVEALREGAAM
                     SRRYEALRSNGVPHSQAIERVFGSGSIGA"
     gene            2902..3156
                     /locus_tag="PK264_10445"
     CDS             2902..3156
                     /locus_tag="PK264_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76346.1"
                     /translation="MTNFSQTLDAPPPAIEGRASDTSRRYRGGLSLTGLLTRLERALY
                     RRLQSERESEIAEFIAANGGDLTDDLERRISQRFGSWAGR"
     gene            3422..5701
                     /locus_tag="PK264_10450"
     CDS             3422..5701
                     /locus_tag="PK264_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRD76347.1"
                     /translation="MTIPTNGPDTLIGTEGPDIIDGLKGNDFIDGLGGDDQLFGNEGN
                     DQVYGRLGNDVLDGGLDDDQVFGGDGNDTITGDLGNDLLEGENGNDTVSGGYGADTIY
                     GGLGDDQLFGNEDADEIYGGDGIDAANGGSGDDRIVGGIGNDILNGGLDKDLVEGGDG
                     DDTVDGELGEDTLYGGLGNDLIRGGEDIDTMYGGDGVDAIYGGLGADIAFGGIGNDTM
                     FGEQDADNLSGEDGDDVLDGGEGDDRLSGGTGNDRLIGGIGNDFMDGGGDTDTVDYRA
                     VTEAISIQLNNNVTDASSAGFGVDTLAFIENAIGGRGNDLIWGTIGVNVLDGFEGNDT
                     IHGLDGNDTLIGGLGNDLLIGGAGGDLHDGGAGTDTVSYAEATARVAAFMTAASLNVG
                     EAAGDTYRSIENMVGSEFADTLMADDAANAVSGGGSHDVLFGLGGADILNGDAGDDSL
                     FGGLGADVHNGGSGFDYARYDFAASGVAVFMPSVYTSFSTGEAAGDTFNSVEGLIGSA
                     FGDTLYGDDNFNILSGLDSNDVLFGNGGGDALYGGNGDDSLFGGIGADALIGGLGGDY
                     ARYDFASGGLTINFANPALNTGEAAGDSYNSVEHVTGSAFADTITGDAGSNVLSGLDG
                     GDTLRGGGSGDALFGLNGDDVLFGESGFDGLFGGAGADQFRFARFENVDQVGDFASGL
                     DKIGLNAASFGGGLVAGGSLAGRFAIDGPTAAVGLFVYATATRTLSWDADGTGGLAAE
                     AVAVLQVGASLVESDLFLF"
     gene            5807..6304
                     /locus_tag="PK264_10455"
     CDS             5807..6304
                     /locus_tag="PK264_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HRD76348.1"
                     /translation="MSDAAASQLAMLERRRIEATILKHVYDTLKASHGIEVAQRAIAD
                     AVRRSSIEQGAEMARSEGRPTSMQTFVDRMQLWTKGNALDMDVKEATATTYRFNVTRC
                     EYANMYKAMGLGEIGHLLSCQRDGTFCEGYDPKLKFKRTQTIMQGAGHCDFEYRYEAG
                     DEDKA"
     gene            complement(6329..7804)
                     /locus_tag="PK264_10460"
     CDS             complement(6329..7804)
                     /locus_tag="PK264_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD76349.1"
                     /translation="MLGRVIVGFIACIVASALPAMASAPKKVALIIGNSGYRNAPPLK
                     NPKSDAELLARTLSDVGFDVTHKTDLTYNDLRIAIRDFGPKAAQSEIAIIHFAGHGME
                     LGGENYVLPIDARLRSDVDLEYEAVTLTSLMRATIGARRLRLVILDACRDNPIGDRMS
                     VTTGATRSFKRGLTRVEPAGDQLVAFAARAGSVALDGDGAISPYTSALAKHLLTPGLD
                     VRLVFGRVRDDVLASTKGQQEPFIYGSLRGDVIPLVASASAETEEAQRERLRAAAEAT
                     AKAKAAELEAKRLIEEAERKRREAEDAAKRVTLELEEAEKRRREAVAEAERRRRELAE
                     EAERLKRESEEEAERRRVAAAEEADRRRREAQEEEERRKAEQLARTFRFRVCNDSRHK
                     AHVAISFYSSEQDGFFVEGWWTVNSGECAYLKRARKGWFYFYADGSNRGWWGGDHSTC
                     VSNRVFLRLHTPNYTCSNNERLVKFKALNIQDDEYTWRLGD"
     gene            complement(7937..8587)
                     /locus_tag="PK264_10465"
     CDS             complement(7937..8587)
                     /locus_tag="PK264_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRD76350.1"
                     /translation="MDAPAQTGAHAGRDARPASAARSRDGRIAVWDPFVRIFHWSLLT
                     LFVVAYLTHDLSETIHQGAGYGIAVLVVLRVVWGLVGSRHARFRDFVYRPTAVLRFLI
                     ATARFKAPRHVGHNPAGGAMVLALLLNIAVICGSGIMMTMDRFWGQKWIEEVHETAVH
                     VCIGLIVLHVLGVIAASLEHRENLVRSMVTGWKRGPAAPAGEQRSIEPTGPSEPAP"
     gene            complement(8587..8862)
                     /locus_tag="PK264_10470"
     CDS             complement(8587..8862)
                     /locus_tag="PK264_10470"
                     /inference="COORDINATES: protein motif:HMM:NF025051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HRD76351.1"
                     /translation="MIPRMITTAVALALLAPAAGAGSYPPGCTTEPRTTWKAESTSAA
                     KATGLDYVVSKTKISGTCHEVYATKGGQKFELFFNPVTNVLVHTTSK"
     gene            complement(8951..10336)
                     /locus_tag="PK264_10475"
     CDS             complement(8951..10336)
                     /locus_tag="PK264_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD76352.1"
                     /translation="MAATGSLRLRLLGAALLAIVLALLASGVLLSRLFALHVERRAAA
                     EMVVHLDQLLAGLEVEAGGAIQVTGALADPRFAKPYGGLYWQIDSPPAARQRSRSLWD
                     FELTLPHDGLATGETHRHNLTGPAGSALFAIERAFSMGPGAAPISFRLTLATDRREID
                     QAAAEFRDTLATSLLVLGVSLLAAFAAMLHFGLAPFRRLGGALQDVHSGKRPKVEGAY
                     PVEVAALVVDVNRLLDKERETIARAGERAGDLAHGFKTPLAVLGAVARDLERDGKSGA
                     ATEIEQQVETMSRHVRRELARARFVGRSGVNPTGIAVGPVVERVIAALERISGERPIA
                     WTVTGPDDCRFAGDETDLFEVIGNLCENASKWARRRADVSIGQAAALLTISVSDDGPG
                     IPEGAEAEALLRGRRIDESGSGSGLGLSIVAKTVDAYGGSISLARGPLGGLMARVVLP
                     AADAAGDITAI"
     gene            complement(10337..10996)
                     /locus_tag="PK264_10480"
     CDS             complement(10337..10996)
                     /locus_tag="PK264_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD76353.1"
                     /translation="MRVLIVEDEARIAEDLRIALDEAGYVSERAADGEAAWFKGDTED
                     FYAVILDLGLPKLDGMTVLRRWREGGRNMPVLVLTARDSWRDKVDGIDAGADDYLAKP
                     FQIEEVIARIRALIRRSNGQASTLLTVDYVSLDLRQRDARIDNRKIALTALEFRCLSY
                     LMHNSGRVVSQSELLEHVYGRDFPHESNALEVLISRLRRKLGLAFIRTKRSEGYVVGG
                     N"
     gene            complement(10999..11340)
                     /locus_tag="PK264_10485"
     CDS             complement(10999..11340)
                     /locus_tag="PK264_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76354.1"
                     /translation="MTKSPFLSALAIIIAALLTVAPGFGDDKPRRTERYGDIEIDQDQ
                     ALALLKSKDFRPLDEILAAAVKVIPGRVIRIKAKRVNGRIAYELKIVSQSGALREIYI
                     DPRNLDILKVE"
     gene            complement(11421..>11752)
                     /locus_tag="PK264_10490"
     CDS             complement(11421..>11752)
                     /locus_tag="PK264_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76355.1"
                     /translation="AFADVVIDTVTAHAPNFKRAVIARQVLSPLDLEERFGLVDGDIF
                     HGQLGLDQLFSMRPVLGHANYRMPLPGLYLCGSGAHPGGGVTGAPGHNAAREIIRDAR
                     RRRWRQH"
     assembly_gap    11753..11805
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11806..>12059)
                     /locus_tag="PK264_10495"
     CDS             complement(<11806..>12059)
                     /locus_tag="PK264_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76356.1"
                     /translation="KTGSGTFRMNVALSELPDFTCRPGNERADHHASGIVIGPTIQYL
                     EDAYLDARRYGWSVKPVVEMLIPSTLDATLAPTGRHVASL"
     assembly_gap    12060..12123
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12124..13027)
                     /locus_tag="PK264_10500"
                     /pseudo
     CDS             complement(12124..13027)
                     /locus_tag="PK264_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046031.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            13221..13598
                     /locus_tag="PK264_10505"
     CDS             13221..13598
                     /locus_tag="PK264_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRD76357.1"
                     /translation="MAKKLLIIMANTDPRNGEELGAPLFQASVAAAMEYEVEVICTAT
                     SGRLMKKGVAEKLVVKPGSPKTVYDFIKDAHEAGVKFLCCSPNLDLFDMTKDELIPEC
                     SGIVGGAYLVEEVMEGDAKVMTY"
     gene            complement(13755..>14563)
                     /locus_tag="PK264_10510"
     CDS             complement(13755..>14563)
                     /locus_tag="PK264_10510"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRD76358.1"
                     /translation="PAGTLANLAPVTRKMTVQLGDGPLLAERTGVDVVYDLRAADAEA
                     GGEGAPLVPVYHRALIAKLRERPVAVLNIGGVANVTWIGRGGELIAFDTGPGNALIDD
                     WMALYTGTSQDKDGAAAARGRIDEDVLAELLSSPYFGAVPPKSLDRNAFSWQPAGKLG
                     VEDGAATLTAFTAASIGRAREHMPEEPKLWIICGGGRRNRTLMSMIAERVENAVVPAE
                     AASLDGDTLEAEAWAYLAVRSLEAQPISYPGTTGVPTPMSGGVLARARSG"
BASE COUNT         2536 a         4838 c         4738 g         2284 t
ORIGIN      
        1 cgcggccagt cctgatatcc gcgcggccta cttgcagagc ggcccgccct tcccccagct
       61 cgagcagtag agcgagcgga cgcgcgtctc cgacgccggc accgggcatt ccgcgcgctt
      121 ttcctggcag cgcagcactt ggcgcgccat tccgacgccg aggcccacca tcgagtgctg
      181 aaactcgacg cggtcgttca cggcatcctg tcgatagtgc ttgtaggtct cgctctcctt
      241 gtcgatgaag aggagacggc gcagacgcgt cagctcgtcg agatcctcga acacgtggat
      301 gatctgcgcc ttagtgttcg gctcggcgag gcggaactgg ttcgtcacaa acgacgagtt
      361 gaagatcgcc gccgagacgc aggggtaagc gtaggcgatg tgctgggcga gaccaccgcc
      421 gagcgagtga ccggcggcga taaaggcgac cttcttcgtg cccgccgtct tgaaggcggc
      481 tttgcgcacc tcgcggaact ccgcgcgcgc ctcgtcgtag cgtgtcgcga tcggggccca
      541 ggccagcgcc caagacatgt tggcgagcca atcagaggca tcgagcgact gcgtgccgcg
      601 gaaggcgacc agcacctcga ggcgcgcggg cgtgtcacgg tggtagacgt ccatcgcgag
      661 cgacgacgac ggcgtgcgcg tatcgatctt ccgccattcc ggcgagtgct tgtcgagcgt
      721 gaagcccttc ggcctgccat ccggatagac gttcagtgcg gcgatggcat acggcaggta
      781 gtcgttggcg gcgacgcggc gatcgtagcc aggccgcaca taaacgggaa gctgtggcgc
      841 ctcgcagcgc gccgcgtcgg cagcgtcgat gccgagttcg gccatggtga gcttcggtcg
      901 gaggagtgcc accccataga ggagggcgac caaggcgatg agccccagcg caagcttctt
      961 catgacgggt ccctcgatgc tgcttcgttg cgaagcgtct ttccgagtcg ccggactggg
     1021 tggcggcggc cctcagaagc gcctcagcgc tccggcaggg aacgtccatt ccggcgtaac
     1081 gattgcgctc ccgcgcgcga ggtcatacca cggtgatccg tccgcggcgg atggatttgt
     1141 gaggatgtgc atttcctgtg caggcatgaa gctctgaagc agcaggaagc ggcgcgcccc
     1201 gctcggcagg atcgccgcgt cgacgatcag caccgcgtgg cccgggaatc cccctttgat
     1261 gatgacgtcg ccgacggacg gctccgccac gggcttcagc tcgcgctcga gcgaggccgt
     1321 gccggcatag gagaagatcc gatccatgta gcggcggaac gagggatagg tggcgtcggc
     1381 cttggccgat tgcgaccagc tgatgccgcc tcgctccgcc cgtggccgcc agccgtcggc
     1441 ccagcgggca aaagagacac gtccgccgcc tgtgaaattg aaggcgatct cgccgacacg
     1501 cccgctcgcg agcagccatt ccgcgcggag cctcatgatg gcatcagcgc actgctgcag
     1561 atcgcgcgtg ccgacgtcga tgtcgatcac ggcaatgtga acgtcctggc gcggcttcgc
     1621 ccgtccgtcg aagagaagca cgggcgctcc tttcggcttc agcggcaggc cacgcagcca
     1681 ggccccgaag ctgccaggcg ccagcgtctc gcgcacggct cccgcaggcg gcgggatgcg
     1741 ctcgacaagg ctctcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnctc ggcgacggcc ggcgccacat gtccaatgat cagaccggcc gaaactgtgc
     1861 cagccaggac agcgacggtg ggttgtcgaa actgcatgcg tggcctcact tcggcatgcg
     1921 gaccgtggaa aactgggtcg cgcggatcga gtgggacttg gaattgagac gcggcgaagg
     1981 ccaatctggc gccaatgcgt ttcctcgtcg agcgatttgc tgcaacgcga tagaaaatga
     2041 tcaacattca ggcaaagtga tgccgcccga gcggcgtgat tcggaatggt attccaaaaa
     2101 atcaagacgg acctgcagct atgcgtcttc cgcatgtcat gttgcgatgc gatattcacc
     2161 tcaacacgaa gaaccgaacc aatcggtcgc agcagggagg tgccgcccag ggctcgatgt
     2221 gaagctcgat ttgcggagac ctgaccatga ccaccctcag cacgactgtc gccaccgcct
     2281 ctcacggtgt tgccgcggac gcgaatttcg aggggaaaca gcacgtcggc cgccccatcc
     2341 tcacccggat cggtgacggc ttcgtcggac tgtaccgcct cctcacggcc ggccaagagg
     2401 cgcgtgcgaa ccgcttcgtc cgcccccatc tcgcgcgcca ggacgacgcg accctgaaga
     2461 ccctcggcct cagcgacgcc gagatcgcca agctgcgcca cgaggcagcc gatatgccgc
     2521 gcatctggct ctgatcgggc gacggcccga tccagcctga gtgccaacca tttcccgaag
     2581 cggcacggca atggccggcc gcaacagaaa gctcaagcca tgaagacaac gaccattgat
     2641 catgccttcc cctcgctctc ggcgacgacg gaggctagca gcgcggtgcg caggcccatc
     2701 ctcgagaagc tgcgcgccta tgtcgaggcg ctccgcgaag gagccgcgat gagccggcgc
     2761 tacgaggcgc tccgcagcaa cggagtgcca cactctcagg cgatcgagcg cgtgttcggc
     2821 agcggttcga tcggggcctg aaacaacgac ggcggagcat tctgccgccc acgcatcacc
     2881 tcgagcagcg aggaacagac catgacgaac ttctcgcaga ccttggatgc gcctcccccc
     2941 gcgatcgagg gccgcgcatc ggacacatca cgccggtatc gcggaggcct gagcctcacc
     3001 ggcctcctca cgaggctcga acgggcactt tatcgtcgcc tgcaatccga gcgcgagagt
     3061 gagatcgccg agttcatcgc cgccaacggt ggcgatctca ccgacgacct ggagcggcgc
     3121 atcagccagc gcttcggctc atgggccggc cggtgacacc gtgaaccgcg gagccccctc
     3181 ggggtgacct gacgggcgcg cgcttgaatt ttcctcgcga tactccgggc accgtcacat
     3241 ggccgtcatc tcggcaggtg tgacggtgcc cgctcaaatt caagtcgaga cgcactccta
     3301 tcaaggcctg gaatagctcc caaccgcagt cgttctcccg gcaacaccgt cgccgccctg
     3361 ccggaacagt tccggcgctc caggccgcga ccgtgagcat gagccaagcc agggagctgc
     3421 catgaccatc ccgacaaacg gaccggacac cctcatcggc actgaaggcc ccgacatcat
     3481 cgacggcctc aagggcaacg acttcatcga cggcctcggc ggcgacgatc agctgttcgg
     3541 caacgagggt aacgaccagg tctacggccg cctcggcaat gatgtcctcg acggcggcct
     3601 cgacgacgac caggttttcg gcggcgacgg caacgacacc ataaccggcg acctcggcaa
     3661 cgaccttctc gagggcgaga acggcaacga cacggtgagc ggcggctacg gcgccgacac
     3721 gatctacggc gggctcggcg acgatcagct cttcggcaac gaggatgccg acgagatcta
     3781 cggcggcgac ggtatcgacg ccgcaaacgg cggctcgggc gacgaccgca tcgtcggcgg
     3841 catcggcaac gacatcctga acggcggcct cgacaaggat ctcgtcgaag gcggcgacgg
     3901 cgacgatacc gtcgacggcg aactcggcga ggacacgctc tacggcggcc tcggcaatga
     3961 cctcatccgc ggcggcgagg acatcgacac gatgtacggc ggcgacggcg tcgacgccat
     4021 ctatggcggg ctcggcgccg acatcgcttt cggcggcatc ggcaacgaca ccatgttcgg
     4081 cgagcaggat gctgacaacc tcagtggcga ggacggcgat gacgtcctcg acggcggtga
     4141 aggcgacgat cggctctccg gcggcacggg caacgaccgg ctgatcggcg gcatcggaaa
     4201 cgacttcatg gacggcggcg gtgatactga cacggtcgac tatcgcgcag tgaccgaagc
     4261 gatcagcatc cagctcaaca ataatgtcac cgacgcctcg agcgccggct tcggcgtcga
     4321 tacgctcgcc ttcatcgaga acgccatcgg cgggcgcggc aacgatctga tctggggcac
     4381 gatcggcgtc aatgttctcg acggcttcga aggcaacgat accatccacg gactcgacgg
     4441 caacgacacg ctgatcggcg gcctcggcaa tgatctgctc atcggtggcg ccggtggcga
     4501 cctgcacgac ggcggcgccg gcaccgatac cgtaagctac gccgaggcga ccgcccgcgt
     4561 cgcggccttc atgacagcgg catctctcaa cgttggcgag gccgccggcg atacctaccg
     4621 ctcgatcgag aacatggtcg gctccgagtt cgccgacacg ctgatggcgg acgacgctgc
     4681 caacgcggtc tccggcggcg gctcgcacga cgtgctcttc ggtctcggcg gcgccgatat
     4741 cctcaacggc gacgccggtg atgacagcct gttcggcggc ctcggcgccg atgtccacaa
     4801 cggcggctcc ggcttcgact acgcgcgcta cgacttcgct gcttccggcg tcgccgtgtt
     4861 catgccgtcg gtctatacga gtttcagcac gggcgaggcg gctggagata ccttcaacag
     4921 cgtggagggg ctgatcggct ccgccttcgg cgacacgctc tacggcgacg acaacttcaa
     4981 catactctcc gggctcgact cgaatgatgt gctgttcggc aacggcggcg gcgatgcgct
     5041 ttacggcggc aacggcgacg acagcctctt cggtgggatc ggcgccgacg cgctgatcgg
     5101 cggcctcggc ggcgactacg cccgctacga cttcgcctcg ggcggcctca ccatcaactt
     5161 cgcgaaccct gccctcaaca cgggcgaggc cgcgggcgac agctacaatt cggtcgagca
     5221 cgtcaccggc tcggccttcg ccgacacgat cacgggcgat gccggctcaa acgtgctctc
     5281 gggcctcgat ggcggcgaca cgctgcgcgg aggcggcagc ggcgacgccc tcttcgggct
     5341 caacggcgac gacgttctct tcggcgagag cggcttcgac ggccttttcg gtggcgcggg
     5401 tgcggaccag ttccgcttcg ccaggttcga gaacgtcgac caggtcggtg acttcgcctc
     5461 tggcctcgac aagatcgggc tgaacgccgc gagcttcggc ggcgggctcg ttgccggcgg
     5521 gtcgctcgca ggtcgcttcg ccatcgacgg accgacggcc gcagtcggcc tgttcgtcta
     5581 cgcgacggcg acgcggaccc tctcatggga cgcggacggc acgggtggcc tggcggccga
     5641 ggcggtcgca gtgctccagg tcggcgcaag cctcgtcgag agcgacctct tcctgttttg
     5701 agccagcgaa gcaaaccagc atctcggtgg cgacgccgag tgactacccc gaggcggcgg
     5761 ttgtgggtta gggtccacac ccatgccgcc gccaagggag cccatcatgt ccgacgccgc
     5821 cgccagccag ctcgccatgc tcgagcgccg tcgtatcgag gcgacgatcc tgaagcacgt
     5881 ctacgacacg ctgaaagcga gccacggcat agaggtcgcc cagcgcgcga ttgccgacgc
     5941 cgtgcgccgc tcctcgatcg aacagggcgc cgagatggcg cggagcgaag gccggcctac
     6001 cagcatgcag accttcgtcg atcgcatgca actctggacc aagggcaacg cgctcgacat
     6061 ggacgtcaag gaggcgaccg ccaccaccta ccgcttcaat gtcacgcgct gcgagtacgc
     6121 caacatgtac aaggcgatgg gcctcggcga gatcggccat ctcctctcct gccagcgcga
     6181 cggcacgttc tgcgagggtt acgacccgaa gctcaagttc aagcgcacgc agacgatcat
     6241 gcagggcgcc gggcactgcg acttcgagta caggtatgag gcgggcgacg aggacaaggc
     6301 atgacgtgac aggcaggtgg cgattggttc agtctcccag tcgccacgtg tactcgtcat
     6361 cttgaatgtt gagggccttg aatttcacca gccgctcatt attgctgcac gtgtagttcg
     6421 gtgtgtgcag cctgaggaac acacgattgc tgacacaggt cgagtgatcg ccgccccacc
     6481 agcctcgatt gctgccgtcc gcgtaaaagt agaaccagcc ctttcgagcc cgcttcaggt
     6541 aagcgcattc gccgctgttg accgtccacc agccttcgac gaagaagcca tcctgctcac
     6601 tcgaatagaa agagatggcg acgtgggctt tgtgacgtga atcattgcag acccggaaac
     6661 gaaacgtcct ggcgagttgt tccgccttcc gccgctcttc ttcctcctgg gcttcccgcc
     6721 gccgacgatc tgcctcttcc gccgccgcga cccgacgccg ctctgcctcc tcttcgctct
     6781 cgcgtttcaa gcgctccgcc tcctccgcga gttcgcgacg gcggcgctcc gcctctgcca
     6841 cagcctcgcg ccggcgcttc tccgcctcct cgagctcgag cgtgacgcgc ttggcggcgt
     6901 cctcggcctc gcgccgcttc ctctccgcct cctcgatcag gcgcttggcc tcgagctcgg
     6961 cggccttggc cttagccgtc gcctcggctg cggcccggag ccgctcgcgc tgcgcttcct
     7021 cggtctcggc gctcgccgag gcgacgagcg ggatcacgtc gccgcgcagc gacccataga
     7081 tgaacggctc ctgctggccc ttcgtcgacg cgagcacgtc gtcgcggaca cggccgaaga
     7141 cgaggcgcac atcgagaccg ggcgtcagca ggtgctttgc caaagccgag gtgtaaggac
     7201 tgatcgcgcc gtccccatcg agggcgacgc tgcctgcgcg cgcagcgaac gcgaccagct
     7261 gatcgcccgc cggctcgacg cgcgtcaggc cgcgcttgaa gctccgcgtc gcccccgtcg
     7321 tcacgctcat gcgatcgccg atcgggttgt cgcggcaggc gtcgagaatg acgaggcgga
     7381 gccgccgtgc gccgatcgtc gcgcgcatca gcgaggtgag agtcaccgct tcatactcga
     7441 ggtcgacgtc gctcctgagg cgcgcatcga tcggcagcac gtagttctcg ccgccgagct
     7501 ccatgccatg gccggcgaag tggatgatgg cgatctcgct ctgcgcagcc ttcggtccga
     7561 aatcgcggat ggcgatgcgc aggtcattgt aggtgaggtc ggtcttgtgc gtgacgtcga
     7621 agccgacatc ggagagcgtg cgggccagca gttcggcgtc actcttcggg ttctttaacg
     7681 gcggcgcatt gcgataaccc gagttgccga tgatcagcgc gaccttcttc ggagcgctgg
     7741 ccatcgcggg cagcgcgctc gcgacgatac acgcgatgaa cccgacgatc acccgaccca
     7801 gcatgtcgac cacccgcgca attgaccggg gccgaggtta ttccaggccc tagtggggac
     7861 gcaacggaca tcccggagtg agaggtcccg cggaaggcgg cgtgcgacga gcaccgcggt
     7921 gctggtcgca ccccgatcat ggcgcgggtt cggaaggacc tgtcggctcg atcgaccgct
     7981 gctcgcctgc cggcgcagcg ggaccgcgct tccagccggt gaccatggag cggacgaggt
     8041 tctcgcgatg ctcgaggctg gccgcgatca cgccgagcac gtggagaacg atgaggccga
     8101 tacagacatg cacggccgtt tcgtgcacct cctcgatcca cttctgcccc cagaaccgat
     8161 ccatcgtcat catgatgccg gagccgcaga tcacggcgat gttcaggagg agcgctagca
     8221 ccatggcacc gccggccgga ttgtgcccga cgtgacgggg cgccttgaag cgcgcagtcg
     8281 caatcaggaa gcgcagtacg gccgtcggcc gatacacgaa atccctgaag cgcgcgtggc
     8341 ggctgccgac gagcccccag acgacgcgca gaacgacgag cacggcgatt ccgtagcccg
     8401 cgccctgatg aatggtctcg gagagatcat gcgtcaggta ggcgacgacg aagagcgtga
     8461 gcagcgacca atggaaaatg cggacgaaag gatcccagac ggcgatcctc ccgtcgcgtg
     8521 accgggcggc cgaggccggc cgcgcatcgc ggccggcgtg ggcaccggtt tgtgctgggg
     8581 catccatcac ttcgacgtcg tgtggacgag cacgttcgtg accgggttga agaacagctc
     8641 gaacttctgc ccgcccttcg tggcatagac ctcgtggcat gtgccggaaa tcttcgtctt
     8701 ggagacgacg tagtcgaggc ccgtcgcctt ggcagcagac gtgctctccg ctttccaggt
     8761 cgtgcgtggc tcagtcgtgc accctggcgg ataggagccg gcgccggcgg ccggagcaag
     8821 aagggcgagt gcaacggcag tggtgatcat tcgcgggatc atggtggtca tctcctggtg
     8881 ttgagccggc ctctcgagcc gacgaggaca ccttaggaga gcggcccgta cctcttgctg
     8941 actccgatcg tcagatagcg gtaatgtcgc cggccgcgtc cgccgcaggt agcacgaccc
     9001 gagccatcag cccgccgagc ggcccccgcg cgagcgagat gctgccaccg taggcatcga
     9061 ccgtcttggc gacgatcgac agcccgaggc cgctgccgct gccgctctcg tcgatacgtc
     9121 ggccgcgcag gagcgcctcg gcttcggcac cctccgggat gccgggacca tcgtccgaca
     9181 ccgagattgt cagcaacgcc gcggcttgcc caatcgagac gtctgcgcgc ctgcgggccc
     9241 acttgctcgc gttctcgcac aagttgccga tcacctcaaa aaggtcagtc tcgtcacccg
     9301 cgaaccggca atcgtccggt ccggtcacgg tccaggcgat cggccgctcg ccgctgatgc
     9361 gctcgagggc cgcgatgacg cgctcgacca cgggcccaac ggcgattcct gtcgggttca
     9421 cccccgagcg gccgacaaag cgagcccgtg cgagctcgcg gcgcacgtgg cggctcatcg
     9481 tctccacctg ctgctcgatc tcggtggcgg cgcctgactt tccgtcccgc tcgagatcac
     9541 gcgcgaccgc gccgagcacg gcgagcggcg tcttgaagcc atgagccagg tcaccggccc
     9601 tctcgccggc gcgcgcgatg gtctcacgtt ccttgtcgag gaggcggttg acgtcaacga
     9661 cgagcgccgc cacctcgacc ggatacgctc cctcgacctt cgggcgcttc cccgagtgga
     9721 catcctggag tgcgcctccg agccgacgga agggcgcgag cccgaagtgg agcatggcgg
     9781 cgaaagcagc cagcagcgag acgccgagca ccagaagcga tgtcgccaga gtgtcacgga
     9841 actccgccgc cgcttggtcg atttcgcggc gatcggttgc gagcgtcagg cggaagctga
     9901 tcggcgcagc acccgggccc atagagaatg ctcgctcgat cgcgaagaga gccgagccgg
     9961 cgggccccgt gagattgtga cggtgcgttt cgccggtcgc caagccgtcg tggggtagag
    10021 tgagctcgaa atcccagagc gacctcgatc gctgccgggc ggcaggaggg ctgtcgattt
    10081 gccagtagag accgccatag ggcttcgcaa accgcggatc ggccagcgct cccgtcacct
    10141 ggatcgcgcc gcctgcctcg acctccaggc cggcgagcag ctgatcgaga tgcaccacca
    10201 tctcggcggc ggcacgccgc tcgacgtgca aggcaaagag gcgcgacagc aacacgcccg
    10261 aggccagcag cgcgagcacg attgcgagca gggcagcgcc cagcagacgc agtcgaagcg
    10321 acccggtcgc ggccaatcaa ttccctccga ccacgtagcc ttcactgcgc ttggtgcgga
    10381 tgaaggcaag cccgagtttg cgccggagac gcgagatgag cacctcgagc gcattgctct
    10441 cgtgcgggaa atcgcggccg tagacgtgct cgagaagctc gctctggctc accacccggc
    10501 cggagttgtg catgagatag ctcaggcagc ggaattccag cgccgtgagc gcgatcttgc
    10561 ggttgtcgat ccgcgcgtct cgctggcgga gatcgagcga cacgtagtcg accgtgagaa
    10621 gcgtcgaggc ctggccgttc gagcgtcgga tcagtgcccg gattcgcgcg atcacctcct
    10681 cgatctggaa gggcttggca aggtagtcat cggcaccggc atcgatgccg tcgaccttgt
    10741 cgcgccagct gtcgcgcgcg gtcaggacca ggaccggcat gttgcggccg ccttcgcgcc
    10801 agcggcgcag cacggtcatg ccgtcgagct tcggcagccc gagatcgagg atcactgcgt
    10861 agaagtcctc ggtgtcgccc ttgaaccacg ccgcctcgcc gtccgcagcc cgttccgaga
    10921 cgtagcccgc ctcgtcaagc gcgatgcgca ggtcctctgc aatccgggcc tcatcctcga
    10981 cgatcaggac gcgcatgcct attcgacctt caggatatcg aggttgcgcg gatcgatgta
    11041 gatctcgcgc aacgcgccgg attgcgaaac gattttcagc tcgtaggcaa tgcgcccgtt
    11101 gacgcgcttc gccttgatcc gaatcacacg acccggaatg accttgaccg ccgcggcgag
    11161 gatctcgtcg agcggcctga aatccttgct cttgaggagt gccagcgcct gatcctgatc
    11221 aatctcgata tcgccgtatc gctcggttcg ccgtggcttg tcgtcgccaa agccgggcgc
    11281 gaccgtgagt agcgccgcga tgatgatcgc aagagcggac aagaacggcg acttggtcat
    11341 ggggcggccg ccaatattgg ggtccaggtg cgaggtagca tggcatggcg cagctgacga
    11401 caagctgaca aggctactgg tcaatgctgt cgccaccggc gacggcgcgc atcgcggatg
    11461 atctcgcggg cggcgttgtg gccgggagcg cccgtgacgc cgccgcccgg gtgcgcaccc
    11521 gagccgcaga gatagagccc gggcagcggc atgcggtaat tggcatggcc gaggaccggt
    11581 cgcatcgaga agagctgatc gaggccgagc tggccgtgga agatgtcgcc gtcgacgagg
    11641 ccgaagcgct cctcgaggtc gagcggcgag agcacctgac gggcgatcac cgcgcgcttg
    11701 aagttcggcg cgtgcgcagt gacggtatcg atcacgacgt cggcgaaggc ctnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacag gctcgccacg
    11821 tgacggccgg tgggagcgag cgtcgcatcg agcgtgctcg ggatcagcat ctcgacgacc
    11881 ggcttcaccg accagccgta ccggcgcgcg tcgagatagg cgtcctcgag atactggatg
    11941 gtcggcccta tgacgatgcc cgaggcgtga tgatcggcgc gctcgttgcc gggcctgcac
    12001 gtgaaatccg ggagctccga tagcgcgaca ttcatgcgga acgtgcccga cccggtcttn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnngcttcgg gccgacgttg gcggcaacgg cgcgggcgcg gatcacctcg ccgccggcga
    12181 gcctgacgcc gcaggcgcga ccgctctcca ccaaaacctc gcggacggga cattgcgtgc
    12241 ggatcgaggc gccgcgtgcg atcgcggatc tcgccatggc ctccgtgatc gcccccatgc
    12301 cgccgatcgc gtggccccac actcctgcct tgccgttcac ctcgccgaag cagtgatgca
    12361 ggaggacgta ggcagtgccg ggcgtcgatg gcgccgcata ggccccaacg atgccgtcga
    12421 aggcgaacga ggccttcact gtctcgttct cgaaccactg gtcgagatag tcactcgcgc
    12481 ttttcgtgaa gagatcggcg atgatgcgct ggtcggcgag cgacagccca aagaggcggc
    12541 gtccgagccc cgcctgccgc gcgatttcgg cgatcccgcc acccgcatta ggcggagtgc
    12601 gcagcacgag atcgcgcagc aacgccgccg cccgctcgag ggctgcatcg taggccggga
    12661 gccgctcggc atcagagggc gaaaacgctg caatggcgcg ctgccggttg gcgagcccgt
    12721 agggcatgac gaggccgcgc gttgcgtcga ccggccaaaa attggcgacc ggccgctcga
    12781 cgatctcgag accgtggcgg tgaagctcga ggtcacggat caccttcggg ttgagcagac
    12841 tcaccgtgta cgaggcgacg gagttgcgaa agcccggatg aaactcctcc gtcaccgcgg
    12901 cgccgccgac gatctggttc ttctcgatga cgaccaccga cagtccggcc gccgccacgt
    12961 agccggccgt cacgaggccg ttgtggcccg caccgatgat ggcgagatcc cagaccgtct
    13021 ccgccatgct gcaacgttct ccagcttcgt tcgacgcgca gatatgcgcc acgcgcgcgc
    13081 tccgggaaag atgcgatgcg gccgctgccc gtccgctgac gcctacggcg cgcgaccttt
    13141 ggatattcgc gcccgcgaat attgccttgc cgcgccggca acgggagcgc tagggttctc
    13201 gcgatacgga ggagaccacc atggcaaaga agctgttgat catcatggcc aacaccgacc
    13261 cgcgcaacgg cgaggagctc ggcgcaccgc tcttccaggc ctcggtggcg gcggcgatgg
    13321 agtacgaggt cgaggtgatc tgcacggcga cctcggggcg cctcatgaag aagggcgtcg
    13381 ccgagaagct cgtcgtcaag cccggcagcc cgaaaaccgt ctacgacttc atcaaggacg
    13441 cgcacgaggc gggcgtgaag ttcctctgct gttcacctaa cctcgacctc ttcgatatga
    13501 ccaaggatga gctcatcccg gagtgctccg gcatcgtcgg cggcgcctac ctcgtcgagg
    13561 aagtgatgga gggcgacgcc aaggtgatga cctattgagg ctgccgaagc cgccgacaca
    13621 ggtgggctca ggtagagctc ctcggtcatg gaccgaagcc gacactcgac ggcgcaacgg
    13681 gcgctgccgt cacgatggac gtgtgaattc agcacgcccc ttcgttcgcc tgggacgagg
    13741 ccctgcacac cggattatcc tgaccgagct cgcgccagca cgcctccgct catcggagtc
    13801 ggcacgccgg tagtgcccgg atacgagatc ggctgagcct cgaggctgcg gaccgcaaga
    13861 tatgcccagg cctccgcctc gagggtatcc ccgtcgaggc tcgccgcctc cgccggcacg
    13921 acggcattct cgacccgctc ggcaatcatg gacatgagag tccggttgcg ccggccgccc
    13981 ccgcagatga tccagagctt tggctcctcc ggcatatgct cgcgcgcccg gccgatcgag
    14041 gccgcggtga aggccgtgag cgtcgcggcg ccatcctcaa cgccaagctt gcccgccggc
    14101 tgccagctga aagcattgcg atccagcgac ttcggcggca cggcgccgaa atagggcgag
    14161 gagagcagct cggcgagcac gtcctcatcg atccgcccgc gtgcggcggc ggcgccgtcc
    14221 ttgtcctggc tcgtgccagt atagagcgcc atccagtcgt cgatcagcgc attccccggg
    14281 ccggtgtcga aggcgatgag ctcgccgccg cgcccgatcc aggtgacatt cgcgacgccg
    14341 ccgatattca agacggcaac gggtcgctcg cggagcttgg cgatcagcgc ccgatgatag
    14401 acgggaacga gcggcgcgcc ctcgcccccc gcctccgcgt cggccgcgcg gagatcgtag
    14461 acgacgtcga cgcccgtccg ctcggcgagc aacggaccgt cgccgagttg cacagtcatt
    14521 ttgcgcgtga ccggcgccag attggcgagc gtgccggcgg gca
//
LOCUS       DAORNH010000085        14558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3133_length_14558_cov_2.695098, whole genome shotgun sequence.
ACCESSION   DAORNH010000085 DAORNH010000000
VERSION     DAORNH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14558
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3133_length_14558_cov_2.695098"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PK264_10515"
     CDS             <1..716
                     /locus_tag="PK264_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD76359.1"
                     /translation="PDLFNILLQVMDHGKLTDHNGKQVNFRNTILIMTTNAGASDLAK
                     AAIGFNRTKREGDDTEAINRMFTPEFRNRLDAVVPFSSLPPEVIAKVVEKFIFQLEAQ
                     LADRNVTIEISDAATAWLAETGYDEKFGARPLARVIQERVKKPLAEELLFGKLEFGGT
                     ARISLVTEPEKKLGFEYVPLDPSKRPKARDEDDDEDTPDGELLVGAAARKALPSPKEK
                     REKPKSGPTGNGSTKRREE"
     gene            complement(945..>1281)
                     /locus_tag="PK264_10520"
     CDS             complement(945..>1281)
                     /locus_tag="PK264_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD76360.1"
                     /translation="FISHNLAVVEHISHRIAVMYLGRIVEYAEKTTLFTRPGHPYTVA
                     LLSAVPVPDPKIKRQKLVLEGDVPSPVRPPPGCHFHTRCPLAQDRCKQETPKLRDIGG
                     GQLVACHLK"
     assembly_gap    1282..1345
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1346..1983)
                     /locus_tag="PK264_10525"
                     /pseudo
     CDS             complement(1346..1983)
                     /locus_tag="PK264_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924256.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
     gene            complement(1976..>2693)
                     /locus_tag="PK264_10530"
     CDS             complement(1976..>2693)
                     /locus_tag="PK264_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76361.1"
                     /translation="TSLNPVMTIGDQIGEALILHQAMSREQATARAIEMLKLVRIPEA
                     EQRVREYPHQLSGGMRQRAMIAMALACNPKVLIADEPTTALDVTIQAQILQLILELQQ
                     RLGTSVVLITHDLGVVAETARRVIVMYAGRKVEEASVEELFGNALHPYTHGLMASIPR
                     LGLMRGETTATAERLQEISGIVPALTDLPKGCAFAPRCKFADERCRNERPGYEEKRPG
                     HWAACFNSDTLYGGKAVTHG"
     assembly_gap    2694..2821
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2822..2991)
                     /locus_tag="PK264_10535"
     CDS             complement(<2822..2991)
                     /locus_tag="PK264_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD76362.1"
                     /translation="MLVAEGLKTYFYTRLGIVKAVDDVSFSVMPGETLAIVGESGCGK
                     SITALTLMRLIP"
     gene            complement(3144..4088)
                     /locus_tag="PK264_10540"
     CDS             complement(3144..4088)
                     /locus_tag="PK264_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76363.1"
                     /translation="MAESRQDSELLARAGANVNGPLGQLLFFVRRYPLGAAGGLIVAA
                     IIFIALFAGVLAKHDPTAIVAKLPLLPPGGEYSLGTDDKARDVWARIAHGARISLMVG
                     IGATLLGCLIGTVIGLISGYFGGWLDLLVQRVMDIMQALPLLVMALLMAVSIGPKMTE
                     ILLAMKVPAIVAGIMPTIIAIAIPTVPNVARIIRSNTLALREMPYVEAARAVGMNEVV
                     IAFRHVLPNTLAPLIVLATAQLGSAILVEASLSFLGLGIPEPHSSWGRMLSESSADYM
                     RQAPWLVIFPGIAISLTVFGMNLLGDALRDLLDPRQRS"
     gene            complement(4093..>4391)
                     /locus_tag="PK264_10545"
     CDS             complement(4093..>4391)
                     /locus_tag="PK264_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76364.1"
                     /translation="AKGAAERTVNYKHALRNALLPVVTIIGIEAAFLLGGLIVTETVF
                     NIPGIARFVVEGIKTRNYPIVQNLIMFIAFVVVVVNFLVDITYTALDPRIKIEE"
     assembly_gap    4392..4521
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4522..5043)
                     /locus_tag="PK264_10550"
     CDS             complement(<4522..5043)
                     /locus_tag="PK264_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76365.1"
                     /translation="MLTYIARRFGLMVLTLVGMSILIFALMRMMPANIADIMMDAAGL
                     VDPKQKVKIEEEFGLNKPYVTQYWQWVSGLMRGDFGYSFTSEGPAIDEILPRIPVTAK
                     LAGLALFFSVLFGVPLGVLSAVRQNSIFDYILRVISLSGLSLPSFWLGLLILTAWVNW
                     LGWIPIYSDKQGLL"
     gene            complement(5154..6767)
                     /locus_tag="PK264_10555"
     CDS             complement(5154..6767)
                     /locus_tag="PK264_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76366.1"
                     /translation="MKRFSAAAFAAVSFGIGVGALTIPTPAAAQKAGGILKIYGRDNP
                     PSASIHEEATISVNMPFMAIFNNLVMFDPFKRRENLESVIPELATKWAWSADNLSLTF
                     ELREGVKWHDGKPFTAADVVCTFDMLTGKGPLAKDMRKNPRGVWYFNLEETKAEGDLK
                     VTFKLKAPQPSFLVLLASGYSPIYPCHVAQNIMRTKPIGTGPFKFAEFKRNESIRVVK
                     NADYWKKGQPYLDEIQWRIIENRSTRVLAFQAGEFDLTFDSDIVLTLIDDMRAKAPKA
                     VCDMRPTGVMTNLIINRAAPPFDNAKLREAFTLALDRSQFNDIIGKGKLKVGAAMLPV
                     SEGAWGLPPDEIAKLPGYSPNLTANIEKAKKIMEGLGYSAAKPLKLKVSTRNISIYRD
                     PAVILIDQLKKIYIEGELDVVDTTIWHAKVTRGDYAVGLNLTGIGVDDPDVNFVENYT
                     CKSERNLTKYCNPKADELIFLQSRTVDLAKRRTIIAEIERILVEDGARPIIYHDQGAT
                     CMQPHLKGLQMKENGIYSTWRFDTVWLDK"
     gene            complement(6890..8191)
                     /locus_tag="PK264_10560"
     CDS             complement(6890..8191)
                     /locus_tag="PK264_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76367.1"
                     /translation="MTERTGQDALKPAHAPERVSLTIMAMALLPFSLGYFLSYLYRAV
                     NAVVAPDLITEFKLSAGELGLLTAAYLLAFALFQLPLGILLDRYGPRRVQAALVAIGG
                     LGALMFAYAPNVYVLTVARAIIGLGFAGGLMSGFKAVVLWVPEQRRALANSLVMSIGA
                     LGLLVSTSPTEWAVQTYGWRNAFLGVVSFTALVAAMIYLVVPERRQQAEPAPLSAQLA
                     DVGQIYRDRVFLGLAPLLATTAGAQIGLQTLWAGPWLKDVAGLDRTGVANVLFISAAG
                     FFAGILISGAVADWLSRRGVSLITVMLGFLAVYVLAQIAIILPIRGYDAWLWSLFGMC
                     GQVAVLAYPWLSSYFGVKLSGRSNTAANLLMFLAAFAIQYVVGVVIDLWPRTASGGYP
                     VEAYSAAFAGTLAIQLLAFFWYLANRRRIAAAEAQMRAERL"
     gene            complement(8374..9030)
                     /locus_tag="PK264_10565"
     CDS             complement(8374..9030)
                     /locus_tag="PK264_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRD76368.1"
                     /translation="MHFYSTERVALFIDGANLYATAKALAFDIDYKRLLGYFRSKGVL
                     VRALYYTALAEDQEYSSIRPLIDWLDYNGYSMVTKPTKEYTDSLGRRKIKGNMDIELT
                     VDAMELCDRLDHIVLFSGDGDFRCLVAALQARGRRVSVVSTLQTQPPMIADELRRQAD
                     QFIDLADLEKEICRDPSTRAPREPREARGAYRERPARGERSEPYPAYQSAGPEDPLDI
                     "
     gene            9259..9807
                     /gene="folK"
                     /locus_tag="PK264_10570"
     CDS             9259..9807
                     /gene="folK"
                     /locus_tag="PK264_10570"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRD76369.1"
                     /translation="MRGETALLAFGANVPGRFGMPTAAIGGALRRLVRRGFTVEAVSK
                     TYTTAPLGPGPQPAYVNAAAVGHWPGGTLALLLAAKRIEREAGRRRGRVWGPRPLDID
                     IIMHGRRSVGWPVRARHEGRLTVPHPHASERSFVITPLLEIAASWRHPISGLRPAELA
                     KRLKPIKGRRNRPRTRPHRAQS"
     gene            complement(9798..10007)
                     /locus_tag="PK264_10575"
     CDS             complement(9798..10007)
                     /locus_tag="PK264_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76370.1"
                     /translation="MKYIVIWGVVAPLSGLAAGILAAARGRDHSYWAAWCFVAPPLLL
                     VLALLPRATFRYTPRRSIDDDDNHD"
     gene            10331..12652
                     /locus_tag="PK264_10580"
     CDS             10331..12652
                     /locus_tag="PK264_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76371.1"
                     /translation="MMLRRDSTYRQPGLSAEIAARLMQAIAALVICALACLPAIALDI
                     EFGGRRIKREILALYDGRHGPLNETRIHKFAEMPLNHLGFKVIYHDISKGLPPIAEMG
                     RYRGVLTWFLEPLPQPDQLVFWLDQVTRTELSYVMIGEVAPPSPEALEPTVARILARL
                     GLHTSGQFIGLTHRSKVVFADPDMLGFERRIDAVLPGYLVVTAKANEATAHLTLRHSS
                     PRGEVSSDVVATSPRGGYAPHDFTIFYEPNTNRVRWILNPFAFFKKAFGNELFPVPDT
                     TTLAGRRLYFSHIDGDGWNNLTDVEEYRVQNMLSAEVIARDAILPFPDLPVSVGLIAG
                     EADRTLGGLSSSGRIARLLYALPHVEVASHTYSHPYSWRFFEAYERTKELDLIEKAAR
                     PEQPVRERLAEGLMRAVGREPPPSRYDKFFAGSDELPRTFMKRPFDLKTEVAGALAMS
                     ESFAPKGKKAKLYLWSGDTTPFEGAIREVRLAGARNINGGDSRLDIEYPSVAYVPPIS
                     RVVGSERHIYAVNSNENTYTNDWTGPYYGFFMLEHTLRNTDAPRRLKAFNLYYHMYSG
                     EKSAALASVRHFLAMARRQPVIPIAASHYTMIAEDFFAAEIEQVNLSAWAVTARGTMQ
                     TVRFDDAEGFGIDWAQSIGVIGFTRHQKALYISLDAQVTRAIVALQARSDPDRRDEAT
                     PRLVLEGSRWQIRAISQTTSEVCDLIATVEGFGPGDMVWRAPPGKSITATIARGGEPL
                     AAQTATVRPDGLVELRLAAAVIEPAMLRLKCHG"
     gene            12645..14141
                     /locus_tag="PK264_10585"
     CDS             12645..14141
                     /locus_tag="PK264_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76372.1"
                     /translation="MADVATGGHRGLELRPNVIVITALSLAALVLAIFMLPGDTERIA
                     VLERDGHYRQALTALEERFAKGDRRQPTLFQLFKLHERHGDIANARRMLEMLVELRPN
                     DHHLQRQLAEFYKITQDEDSYVRALARQLDIRYSEPACRNLIGIWRRRGEADTEQKSI
                     ELCRSKGYRRTEDIIRLGFLSAAGGNLVAAADILRAVDDRRRLKLDEERHLLFAALVE
                     AGQEKEANRRAGRWLRGQRNDAFALTLIDTLVESRKFDAAVDLAREVGTVGDSVSLVI
                     ASLMTEQGQQVAARAYLRGWLEKAVLETEDITTRFLASALDAEDSELAYIGAQRYGLP
                     KLGQSHLAELAEALGADGRRKEFRSVRSHLAEETVRANPLLAAAIEAERGATVPARVL
                     LSRVNVGELEEWRLALWSRLMETTGRRASPTAVPRPGTGEPTGIGPQSLGPPRIVRRT
                     KDRTRTIRRITRGKAPPPTAPLGPGAVPGPAPGVAPKPFAPPSFSSGG"
     gene            14275..>14558
                     /locus_tag="PK264_10590"
     CDS             14275..>14558
                     /locus_tag="PK264_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76373.1"
                     /translation="MQTRGPESGTPGGMGRHGLPVLPPLVAWLEIALLILPITVYDYL
                     STGFTITELHPHPFWLPVLLLSLQYGTPSGLAAAGVAILATFALGWPEQD"
BASE COUNT         2559 a         4620 c         4686 g         2371 t
ORIGIN      
        1 atccggacct gttcaacatc ctcctgcagg tgatggacca cggcaagctc accgaccaca
       61 acggcaagca ggtgaacttc cgcaacacca tactcatcat gacgacgaat gcgggtgcat
      121 ccgaccttgc caaggcggcg atcggcttca accgcacgaa gcgcgaaggc gacgacacgg
      181 aagcaatcaa ccggatgttc acgccggagt tccgcaaccg gctcgacgcc gtggtgccgt
      241 tctcgtcgct gccgccggag gtgatcgcca aggtcgtcga gaagttcatc ttccagctcg
      301 aggcgcagct cgccgacagg aacgtcacga tcgagatctc ggacgccgcc acggcttggc
      361 tcgccgagac cggctacgac gagaagttcg gcgcgcgccc gctcgcccgc gtcatccagg
      421 agcgcgtgaa gaagccgctc gccgaggagc tgctcttcgg caagctcgag ttcggcggca
      481 cggcgcgcat ctcgctcgtc acggagcccg agaagaagct cggcttcgag tacgtgcctc
      541 tcgatccctc gaagcggccg aaggcacgag acgaggatga cgacgaggac acgccggacg
      601 gcgagctcct cgtcggcgca gccgcccgca aggcgctgcc gagccctaag gagaagcgcg
      661 agaagccgaa gtccggcccc actggtaacg gcagcaccaa gcgacgcgag gagtgagggg
      721 cgggcgctgc cggcgccgtt gcgatccaag tcgcggcagt gttcactgcg cgaagcgggg
      781 ctcccgcccc acgccccggt cggagggaca ccctcccaac cacccgttct tgcattcttg
      841 aaatcaaagc gggtggggtt cgggggcggc ctgagccccc gagcgggtct gggcggcagc
      901 ccggtcgcaa agcgaagttg cgaaaatcga caccgccccc ggcgtcactt caaatggcac
      961 gccaccagct gcccgcctcc gatgtcacgg agcttcggcg tctcctgctt gcagcggtct
     1021 tgcgcgaggg ggcagcgggt gtggaagtga cagccgggcg gcgggcgtac ggggcttggg
     1081 acgtcgccct cgagtacgag cttctggcgc ttgattttcg ggtccggtac gggcaccgcg
     1141 gagaggagcg cgaccgtata agggtggccc gggcgcgtga agagcgtggt cttctcggca
     1201 tactcgacga tgcgcccgag atacatgacc gcgatacggt gcgagatgtg ctccaccacg
     1261 gccaggttgt gcgagatgaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnctgcg cctggatcga cacgtcgagc gcggagacgg
     1381 gctcgtcgcc gatgatgagc ctcgggttca atgcgagggc gcgcgcgatg ccgatgcgct
     1441 ggcgctggcc gcccgagaac tggtggggaa agttccccat ctgcgcgcgc cggagcccga
     1501 cgcgatcgaa gagcttggca acggcctccg tacgctccgc tgacgagccg acgccgtgca
     1561 ccttgagcgg ctcgccgacg atgtcgcccg ccgtcatgcg cggatcgagc gaggagaacg
     1621 ggtcctggaa gatgatctgc atgtgccggc ggtgcgggcg cagctcggat ttcggcagcg
     1681 tggagatgtc ctgaccaagc aggcggatcg tgccgctcgt cggctcgacg agacgcagga
     1741 tcggccaacc gtggtcttgc cgcagcccga ttcgccgacg aggccgactg tctcgccctc
     1801 gccgaccgag aacgacacgc cgtcgaccgc gtggacatga ccgacggtct tgcgcagcag
     1861 gcccttcttc accgggaagt gcttcttgag gtcgacgacc tcgagcacgg ggcggcgctg
     1921 agtgctctcg gcggacggcg cggcgccact tgccgcctgt ccgggcgttg caccgtcagc
     1981 catgggtcac cgccttgccg ccatagagcg tatcggaatt gaagcaggcc gcccaatgcc
     2041 ctggccgctt ctcctcgtag cccggccgct cgttgcggca gcgctcgtcg gcgaacttgc
     2101 agcgcggcgc gaacgcgcag cccttgggaa gatcggtaag cgcgggcacg atcccagaga
     2161 tctcctgcag ccgctcggcc gtcgccgtgg tctcgccgcg catcaggccg aggcgcggga
     2221 tcgaggccat cagaccgtgc gtgtaggggt ggagcgcatt gccgaagagc tcctcgaccg
     2281 atgcctcctc gaccttgcga ccggcgtaca tgacgatcac gcgccgcgcc gtctctgcga
     2341 ccacgccgag atcgtgcgtg atgagcacga cggaggtgcc gagccgctgc tgcagctcga
     2401 ggatcagctg gaggatctgc gcctggatgg tgacgtcgag cgcggtcgtc ggctcatcgg
     2461 cgatgagcac cttcggattg caggcgagag ccatggcgat catggcgcgc tggcgcatgc
     2521 cgccggaaag ctggtgagga tactcgcgca cccgctgttc ggcctcgggg atgcgcacga
     2581 gcttcagcat ctcgatggcc cgcgccgtcg cctgctcgcg cgacatggcc tggtgcagga
     2641 tcagcgcctc gccgatctgg tcgccgatgg tcatcaccgg attgagcgat gtcnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nttcgggatg aggcgcatca aggtgagcgc cgtgatgctc ttgccgcagc ccgattcgcc
     2881 gacgatcgcc agcgtctcgc cgggcatgac cgagaacgag acgtcgtcga cggccttgac
     2941 gatgccgagc cgcgtataga agtaggtctt gagaccctcg gcgacgagca acggcgctgt
     3001 tcccgaagtg ctggccgcgg gtcgcggcgc gctcggccca ggggccgtag aggtcgcgct
     3061 gccgctgccg ggcacacctg ccggaggcat gggcggagct gcggccgcgc gggtcgatgg
     3121 ctgtccccaa tcgctcatct cggtcagctc cgctggcggg gatcgaggag atcgcggagc
     3181 gcgtccccga gcaggttcat gccgaacact gtgagcgaga tggcaatgcc cgggaagatg
     3241 acgagccagg gcgcctggcg catgtagtcg gcggagcttt cggacagcat gcgcccccac
     3301 gaggagtgcg gctcgggaat gccgaggccg aggaacgaga gcgaggcctc gaccaggatc
     3361 gccgagccga gctgggcggt ggcgagcacg atgagtggcg cgagcgtgtt cggcagcacg
     3421 tggcggaagg cgatcacgac ctcgttcatg ccgacggcgc gcgccgcctc cacgtagggc
     3481 atctcgcgca gcgcgagcgt gttcgagcgg atgatgcgcg ccacattcgg caccgtcggg
     3541 atggcgatgg cgatgatcgt cggcatgatg ccggcgacga tcgccggaac cttcatggcg
     3601 agcaggatct cggtcatctt cgggccaatc gagacggcca tcagcagcgc catcacgagc
     3661 agcggcagcg cctgcatgat gtccatgacg cgctgcacga ggagatcgag ccagccgccg
     3721 aaatagcccg agatgagacc gatcaccgtg ccgatcaggc agccgagcag cgtcgcgccg
     3781 atgccgacca tcagcgagat gcgcgcaccg tgcgcgatgc gcgcccacac gtcgcgtgcc
     3841 ttgtcgtcgg tgccgagcga atactcgccg ccgggcggca acagcggcag cttggcgacg
     3901 atggcggtcg gatcgtgctt tgccagcacg cccgcgaaga gcgcgatgaa gatgatggcg
     3961 gcgacgatca ggccgccggc cgcgccgagc ggatagcggc gtacaaagaa gaggagctgc
     4021 ccgagcgggc cgttgacatt ggcgcccgcc ctggcgagga gctcgctgtc ctgtcggctt
     4081 tcagccacgc gcctactcct cgatcttgat gcgcgggtcg agcgccgtgt aggtgatgtc
     4141 gaccaggaag ttcaccacga cgacgacgaa ggcgatgaac atgatcaggt tctgcacgat
     4201 cgggtagttg cgcgtcttga tgccctcgac aacgaaacgg gcgatgcccg gaatgttgaa
     4261 caccgtctcc gtcacgatga gcccgccgag caggaaggcg gcctcgatgc cgatgatcgt
     4321 cacgaccggc agcagcgcat tgcggagtgc gtgcttgtag ttgacggtac gctcggccgc
     4381 gcccttggct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn ncaggagtcc ttgcttgtcg gagtatatcg ggatccagcc
     4561 gagccagttc acccaggccg tgaggatcag gagcccgagc cagaacgacg gcagcgagag
     4621 gcccgatagc gagatgacac ggaggatgta gtcgaagatc gaattctggc ggacggcgct
     4681 cagcacgccg agcggcacgc cgaacagcac cgaaaagaag agcgcgaggc cagcgagctt
     4741 tgctgtcacg gggatacgcg gcaggatctc gtcgatcgcc ggcccctccg aggtgaacga
     4801 gtagccgaag tcgccgcgca tcagcccaga aacccactgc cagtactggg tgacataggg
     4861 cttgttgagg ccgaactcct cctcgatctt caccttctgc ttcggatcga cgagaccggc
     4921 tgcatccatc atgatgtcgg cgatgttggc cggcatcatg cgcatgagtg cgaagatgag
     4981 gatcgacatc ccgaccagcg tcaggaccat cagaccgaag cggcgcgcga tgtacgtgag
     5041 cacttaggac gactttggag agttgacgcg aactcgagat gccggtgcgt gcgaggtgac
     5101 gggcgcggct gccacctccg gcgaccgcgc ccgcatttcg tgggcaccct agctcacttg
     5161 tcgagccaga cggtgtcgaa acgccaagtg ctgtaaatgc cgttctcctt catctgcaga
     5221 cccttcaggt ggggctgcat gcaggtggcg ccctggtcgt ggtagatgat cgggcgcgcg
     5281 ccatcctcga cgaggatgcg ctcgatttcg gcaatgatcg tgcggcgctt ggcaaggtcg
     5341 acggtccgcg actgcaggaa gatgagctcg tcggccttcg ggttgcagta cttcgtcagg
     5401 ttgcgctccg acttgcaggt gtagttctct acgaagttca cgtccgggtc gtcgacgccg
     5461 atccccgtca ggttgaggcc gacggcatag tcgccgcgtg tcaccttggc atgccagatt
     5521 gtcgtatcga cgacgtcgag ctcgccttcg atgtagatct tcttcagctg gtcgatgagg
     5581 atcaccgccg ggtcgcggta gatcgagatg ttgcgtgtcg acaccttcag cttcaggggc
     5641 ttggctgcgc tgtagccgag cccctccatg atcttcttcg ccttctcgat gttggcggtg
     5701 aggttcgggc tgtagccggg cagcttggcg atctcatcgg gcggcaggcc ccatgcgccc
     5761 tcggacaccg gaagcatggc cgcgccgacc ttgagtttgc ccttgccgat gatgtcgttg
     5821 aactggctgc gatcgagcgc cagtgtgaag gcctcgcgca gcttcgcgtt gtcgaagggc
     5881 ggcgccgcgc gattgatgat gaggttcgtc atcacgcccg tcgggcgcat gtcgcaaacc
     5941 gccttcggcg ccttcgccct catgtcgtcg atcaacgtca gcacgatgtc actgtcaaag
     6001 gtgagatcga actcgccggc ctggaaggcg agcacgcgtg tcgagcggtt ctcgatgatg
     6061 cgccactgga tctcgtcgag atagggctgc ccctttttcc agtagtcggc attcttcacg
     6121 acgcggatcg actcgttccg cttgaactcg gcgaacttga acggtccggt gccgatcggc
     6181 ttcgtgcgca tgatgttctg cgcgacgtgg cagggataga tcggcgaata gccggaagcg
     6241 agcagcacca ggaacgacgg ctgcggcgcc ttcagcttga aggtgacctt gagatcgcct
     6301 tccgccttcg tctcctcgag attgaagtac cagacgccgc gcgggttctt gcgcatgtcc
     6361 ttggcgagcg gacccttacc cgtcagcatg tcgaaggtgc agaccacgtc agccgcggtg
     6421 aaaggcttgc cgtcgtgcca cttcacgcct tcgcgcagct cgaaggtgag cgagagattg
     6481 tcggcgctcc aggcccactt ggtcgcgagc tcggggatga cgctctcgag gttctcgcgc
     6541 cgcttgaagg gatcgaacat gacgagattg ttgaatatcg ccatgaacgg catgttcacg
     6601 gagatcgtcg cttcctcgtg gatcgaggcg ctcggcgggt tgtcgcgtcc gtagatcttg
     6661 aggataccgc cagccttctg cgcggcggcc ggcgtcggaa ttgtcaaggc gcccacgcca
     6721 ataccgaagg aaaccgccgc aaacgcggca gcagagaagc gcttcatgca gtcctcccga
     6781 gacttgtggc cggtggcctc actttcttat gcgagcacgc tagcatgccc gctcggttga
     6841 gcaacccgga ttcgcgtgga actgttgtcc gcctgacggc aggcagcggt cagaggcgct
     6901 cagcgcgcat ctgagcctcg gcggcggcga tgcggcgccg gtttgcgagg taccagaaga
     6961 aggccaggag ctggatggcg agcgtgccgg cgaaggctgc cgagtaggcc tcgaccggat
     7021 agccgccaga ggcggtccgc ggccacagat cgatgacgac gccgacgaca tactggatcg
     7081 cgaaggccgc aaggaacatg aggaggttgg ctgccgtatt ggagcggcct gacagtttca
     7141 cgccgaagta gctcgagagc catggatagg cgaggacggc cacctgcccg cacatgccga
     7201 acagcgacca gagccaggca tcgtagccgc gaatgggcag gatgatcgcg atctgggcga
     7261 ggacgtagac ggcaaggaat ccgagcatca ccgtaatcag gctcactccg cgccggctca
     7321 gccagtcggc aacggcaccg ctgatcagga tgccggcgaa gaagccagcc gcactgataa
     7381 agaggacgtt ggcaacacct gtgcggtcga ggccggcgac atccttgagc caggggcccg
     7441 cccacagagt ctgcaggccg atctgtgctc cggcagtggt tgcgagcagc ggcgcgaggc
     7501 cgaggaagac gcggtcgcgg tagatctggc cgacgtcggc gagttgcgcg ctgaggggag
     7561 cgggctccgc ctgctggcgg cgctccggca cgacgaggta gatcatcgcg gcgacaagtg
     7621 ccgtgaagga aacgacgccg agaaatgcat tccgccagcc gtaggtctgc accgcccatt
     7681 cggtcggcga cgtcgagacg aggaggccga gcgccccgat cgacatcacc agcgagttcg
     7741 ccagcgcacg gcgctgctca ggaacccaga gcacgacggc cttgaagccg ctcatcaggc
     7801 cgccggcaaa gccgagcccg atgatcgcgc gggcaaccgt caggacgtag acgttcggcg
     7861 cataggcgaa catcagcgcg ccgagcccgc cgatcgcaac cagcgcggcc tgcacgcgcc
     7921 gcggcccata gcgatcgagc aggatgccga gcggcagttg gaagagggcg aaggccagaa
     7981 gataggcggc tgtcagcagg ccgagctcac ccgcggagag cttgaactcc gtgatgaggt
     8041 cgggggcgac gaccgcattc acggcacgat acagataaga caggaagtaa ccgagactga
     8101 acggaaggag cgccatagcc ataatggtga gcgagacgcg ctccggggcg tgcgccggct
     8161 tcagcgcgtc ctgtcccgtc cgttccgtca ttctctcacc tcaatcgacc tggcgcgccc
     8221 gcggctcacg aggttggacc gtacgaagca gctcggcatt cgccaatgcg gatggtgccg
     8281 catgcagggc tggcatgcag agaagggccc gccgccatgt cgcggccgag cccttcggta
     8341 ggtcgacagt tccaggtcgt cgtacgtctc ggatcagata tcgagcggat cttccggtcc
     8401 ggcggactgg tacgccggat acggttcgct acgctcaccg cgcgcgggcc gctcacgata
     8461 ggcgccacgc gcctcgcgag gctcgcgcgg agcgcgggtg gacggatcgc ggcagatttc
     8521 cttctcgaga tcggcgagat cgatgaactg gtcggcctgg cgccgcagct cgtctgcaat
     8581 catggggggc tgcgtctgca gggtggacac cacgctgacg cggcgtccgc gggcctggag
     8641 cgctgcgaca aggcagcgga agtctccgtc acccgagaac aggacgatgt ggtcgaggcg
     8701 atcacacagc tccatggcat cgacagtcaa ctcgatgtcc atattgcctt tgatcttccg
     8761 gcggccaagt gaatccgtgt attccttggt cggcttcgtc accatcgagt agccgttata
     8821 gtcaagccag tcgatgagag ggcgaatcga cgaatattct tggtcttcag caagagctgt
     8881 atagtataag gctcgcacca gaacgccctt agatcgaaaa taaccaagca atctcttgta
     8941 atctatgtca aaggccaagg cttttgctgt ggcgtataga tttgcgccgt ctatgaacaa
     9001 tgctactctt tctgttgagt agaaatgcat gttgttcttc cctgtattta cccgtcaata
     9061 cttctgtaat attttcgaag ttgctacggg tcgggctggg ccctgggttt atcctaatgt
     9121 atcggccgca cgccgcaaat cggcaggacg cacggttgcc agcctataac taggcgttct
     9181 cgcagcgatt gtgtagcacc gatgtaacaa ttcagctttt ctgcacgccg gcaaaccgaa
     9241 cgggaacagc ccacgaaaat gagaggcgaa acagcactgc tggccttcgg cgccaacgtt
     9301 cccgggcggt tcgggatgcc gacagctgcg attggcggag ccttgcggcg gcttgtgcgc
     9361 cgcgggttca cagtcgaagc cgtgtcgaaa acctacacga ctgcgccgct cgggccagga
     9421 ccacagcccg cctacgtaaa cgcagcagcc gtcggtcact ggccgggcgg aacgctcgcc
     9481 ctgctgctcg ccgccaagcg catcgagcgc gaggctggcc gccgccgagg ccgggtctgg
     9541 gggcctcgcc cgctcgacat cgacatcatc atgcatggcc gacgcagtgt cgggtggccg
     9601 gttcgggcgc gccacgaggg gcgactgacg gtgccacacc cccacgccag cgaacgatct
     9661 ttcgtgatta cgccgctcct cgaaatcgcc gcctcctggc gacatccgat atccggacta
     9721 cggcccgccg agctcgccaa gcgcctgaag ccgatcaagg ggcgccgcaa ccggccacgc
     9781 acgcggcctc atcgcgctca gtcgtgatta tcatcgtcgt cgatggagcg cctcggcgtg
     9841 tagcggaagg tcgcccgcgg cagcagcgcg aggacgagca gcaggggcgg ggccacgaag
     9901 caccatgccg cccaatacga gtggtcacgg ccgcgtgcgg cggcgagtat cccggcggcg
     9961 aggcctgaaa ggggcgcaac cacaccccag attacaatgt acttcatcgc aaagccctct
    10021 gctctcgttc gtggttgtcc cgagtaggtt ccacacctga gaagttgttg tcgtaatagt
    10081 tgcggtcgcg agagctccta tcgatcgctc gccgggtggc gagctgggag agaatatgag
    10141 gattcaagga gttgtaatca agcaccctgc cgcaatgatg gtcgaaatcg atagcggcgc
    10201 attttcgctc ccgtcgcgcc ctccgcccac tcttgcgcat cccgagagtg tgctatgcac
    10261 gtccggtcag cggctcgacg ttgcgcccgt tccgcacgcg acgccttgga aaaccggctc
    10321 aagatcgcac gtgatgctcc gacgcgacag cacctatcga cagcccgggc tatcggcgga
    10381 gattgccgcg cgtcttatgc aggccatcgc ggcgctcgtc atctgtgccc tggcgtgcct
    10441 ccccgcgatc gccctcgata tcgagttcgg cggcaggcgg atcaagcgcg agatccttgc
    10501 cctctacgat ggccggcatg gaccgttgaa cgagacgcgt atccacaagt tcgccgagat
    10561 gccgctgaac cacctcggct tcaaggtaat ctaccacgac atctcgaaag gcctgccgcc
    10621 gattgccgag atggggcgct atcggggcgt cctcacgtgg ttcctcgagc ccttgccgca
    10681 gcccgaccag ctcgtcttct ggctcgatca ggtcacgcgc accgaactca gctacgtcat
    10741 gatcggcgag gtcgccccgc cttctcccga ggcactggag ccgacggtcg cccgcatcct
    10801 cgcgcggctc ggcctgcata cgagcggcca gttcatcggc ctcacgcatc gctcaaaggt
    10861 cgtgttcgcc gacccggata tgctcggctt cgagcggcgc atcgacgccg tgctgcccgg
    10921 ctacctcgtg gtgacggcca aggcgaacga ggcgacggct catctcacac tacgccacag
    10981 cagcccgcgc ggcgaggtct cctccgacgt tgtcgcgacg agcccgcggg gtggctatgc
    11041 gccgcacgac ttcacgatct tctacgagcc caacaccaat cgggtgcgct ggatcctcaa
    11101 cccgttcgcc ttcttcaaga aggcctttgg caacgagctg ttccccgtcc cggatacgac
    11161 cacgcttgcc ggccggcggc tctatttcag ccacatcgac ggagacggct ggaacaacct
    11221 caccgacgtc gaagagtatc gcgtccagaa catgctgtcg gctgaggtca tcgcgcgcga
    11281 tgccatcctg ccgtttccgg accttcctgt cagtgtcggc ctcatagccg gcgaggccga
    11341 ccgcactctc ggcggcctgt cctcgagcgg tcgcatcgcc cgcctcctct acgcgctgcc
    11401 gcacgtcgag gtggcgagtc acacctactc gcatccctac tcgtggcgct tcttcgaggc
    11461 ctacgagcgg accaaggagc tcgatctgat cgagaaggcc gcgcgccccg agcagccggt
    11521 ccgcgagcgc ctcgccgagg ggctcatgcg ggcagtcggt cgcgagccgc cgccatcgcg
    11581 ctacgacaag ttcttcgccg ggtcggacga actgccaaga acgttcatga agcggccctt
    11641 cgacctcaag accgaagtcg ctggcgcgct cgccatgtcc gaaagctttg cgccgaaggg
    11701 caagaaggca aagctctatc tttggagcgg cgatacgacc cccttcgagg gcgcgatccg
    11761 cgaggtgcgg ctcgccggcg cccgaaacat caacggcggc gattcacgcc tcgatatcga
    11821 gtatccctcg gtcgcctatg tgccgccgat cagccgcgtc gtcgggtcag agcgtcacat
    11881 ctacgccgtc aacagcaacg aaaataccta caccaacgac tggaccgggc cctactacgg
    11941 cttcttcatg ctcgagcaca cgctgcgcaa taccgacgcg ccgcggcgcc tcaaggcctt
    12001 caatctctac tatcacatgt actccggcga aaaatccgcg gcattggcct ccgtgcgcca
    12061 cttcctcgcg atggcgcgtc gtcagcccgt catcccgatc gccgcctcgc actacacgat
    12121 gattgcggag gacttttttg ccgcagaaat cgagcaggtg aacctgtcgg cctgggccgt
    12181 cacggcgcgt ggcacgatgc agaccgtccg cttcgacgac gccgaggggt tcggcattga
    12241 ctgggcgcag agcatcggcg tgatcggctt cacacgccac cagaaggcgc tctacatcag
    12301 cctcgatgcg caggtgacgc gcgcaatcgt ggccctgcag gcccggtccg atccggaccg
    12361 gcgcgacgag gcgaccccga ggctcgtgct cgagggcagc cgttggcaga tccgcgccat
    12421 ctcgcagacg acatcggagg tctgcgacct gatcgcgact gtcgaaggat tcggccccgg
    12481 cgacatggtg tggcgcgcgc cccccggaaa gtcgatcacg gcaacgatcg cacgaggcgg
    12541 cgagccgctg gcagcacaga cggcgaccgt caggcccgac ggcctcgtcg agctccgtct
    12601 cgccgctgcc gtcatcgagc ccgccatgct gaggctcaag tgccatggct gatgtcgcga
    12661 ccggcgggca ccgtggcctg gagctcagac ccaacgtcat cgtcataacc gcgctctcgc
    12721 tcgcagcgct tgtgctcgca atcttcatgc tgccgggcga cacggagcgc attgccgtgc
    12781 tcgagcgcga tggtcactat cgccaggcac tgaccgcgct cgaggagcgc ttcgcgaagg
    12841 gcgaccggcg gcagccgacg ctgttccagc tcttcaagct ccacgagcgc catggcgaca
    12901 tcgccaatgc ccgccgcatg ctggagatgc tggtcgaact caggcccaat gaccaccatc
    12961 tccagcgcca gctggccgag ttctacaaga tcacgcagga cgaggactcc tacgtgcgcg
    13021 ccctcgcccg ccagctggat atccgctatt cggagccggc ttgccgcaac ctgatcggca
    13081 tctggcggcg gcgaggcgaa gccgataccg agcagaagtc gatcgaactc tgccggtcga
    13141 agggctaccg gcgcaccgag gacatcatca ggctcggctt cctgtcggcc gccggcggca
    13201 atcttgtcgc cgccgccgac atcctgaggg ctgtcgacga ccgccgccgg ctgaagctgg
    13261 acgaggagcg ccatctcctg ttcgccgcgc tcgtcgaggc cggccaggag aaggaggcca
    13321 atcggcgggc cggacgctgg ctgcgaggcc agcgcaacga cgctttcgcg ctcacgctca
    13381 tcgacaccct cgtcgagtcc cgcaagttcg atgctgcggt cgacctcgcg cgcgaggtcg
    13441 gcaccgtcgg cgacagcgtt tcgctggtca ttgcaagcct catgacggag cagggccagc
    13501 aggtcgcggc gcgggcctat ctgcgcggct ggctcgagaa agccgtgctc gagacggagg
    13561 acatcaccac gcgcttccta gcctccgcgc tcgacgcgga agacagcgag ctcgcctaca
    13621 tcggcgcaca gcgatacggc ctcccgaaac tcggccagtc gcacctcgca gagctcgccg
    13681 aggcgctcgg tgcggacgga cggcgcaagg agttccgatc cgtcagatcg catcttgccg
    13741 aggagacggt gcgcgccaac ccgctgcttg cggccgccat cgaggccgag cgcggtgcaa
    13801 ccgtgccggc gcgcgtgctc ctctcgcgcg tcaatgtcgg cgagctcgag gaatggcggc
    13861 tcgccctatg gtcgcggctc atggaaacta ccggccgccg ggccagcccg acggcggtcc
    13921 cgcgcccagg aaccggcgag cccaccggca tcggtcccca gtccctcggg ccgccgcgaa
    13981 tcgttcggcg gaccaaggac cgaacccgaa ccatccgccg gatcacacgg ggcaaggcgc
    14041 cgccgcctac ggcgccgctc ggaccgggcg cagtccccgg gccggcaccg ggtgtggctc
    14101 cgaagccatt tgcgccgcca tcgttcagca gcggcgggta ggcgaactct ttgggcggat
    14161 gcttattgat cgccggatgc gaccctccag tcattgcgga aatgcgcaac cgccgatagc
    14221 gtgccgggct ggcgcgacag atctccgcca tcaatcgatc attggagtgg acgagtgcag
    14281 acgagaggtc cagagagcgg cactcctggt ggaatggggc gccatgggtt gcccgtgctg
    14341 ccgccgctcg tcgcctggtt ggaaatcgcg ctgctgatcc tgccgatcac cgtctacgac
    14401 tacttgtcca ccggcttcac gataacggag ttgcacccgc atccattctg gctgcccgtg
    14461 ctgcttctga gcctccagta cggcaccccg tccgggctcg ccgctgcagg tgtcgccatc
    14521 ctcgcgacct tcgcgctcgg ttggccggag caggacat
//
LOCUS       DAORNH010000086        14552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3137_length_14552_cov_2.328413, whole genome shotgun sequence.
ACCESSION   DAORNH010000086 DAORNH010000000
VERSION     DAORNH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14552
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3137_length_14552_cov_2.328413"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>209)
                     /locus_tag="PK264_10595"
     CDS             complement(<1..>209)
                     /locus_tag="PK264_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRD76374.1"
                     /translation="GSHGVPYHVIVDNAGGHYMQRGEVDLVIVGTDRVTRRGDVANKI
                     GTYLKALAAKDTGVPFYVALPHSTI"
     assembly_gap    210..248
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<249..836)
                     /locus_tag="PK264_10600"
     CDS             complement(<249..836)
                     /locus_tag="PK264_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRD76375.1"
                     /translation="MRIAGRHYRTIWPTADGRAVEIIDQTRLPHQFVTKVLATADEAA
                     RAIKTMEVRGAPLIGATAAYGLAMALRRDPSDRALEASIEHLASQRPTAVNLRWALDE
                     MRKAVAPLAPTARANAAWLKAAAICDDDVETCRRIGEHGLGLIKAAAKRKAGKPVNIL
                     THCNPGWLATVDYGTATAPIYLAQDEGLPVHVWVDE"
     gene            complement(836..1366)
                     /locus_tag="PK264_10605"
     CDS             complement(836..1366)
                     /locus_tag="PK264_10605"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HRD76376.1"
                     /translation="MQDLRSLVRTIPDYPKPGIMFRDVTTLLADPQGFKAAIARMAES
                     YRTEPVDAVAGIEARGFILGGAVADRLGCGFIPVRKKGKLPWKTISQSYQLEYGEDTI
                     EIHVDAISKGARILIVDDLIATGGTAEAAVKLVQRAGGTVLGASFVIDLPELGGVKRL
                     ARLGVESKALMAFEGH"
     gene            complement(1414..3222)
                     /locus_tag="PK264_10610"
     CDS             complement(1414..3222)
                     /locus_tag="PK264_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD76377.1"
                     /translation="MKRTLSKPAISALCALLALVGTAVTAFARGGVADLPDSKSLLGS
                     YLAGRYARALHETDAAAALYRRALAQDPANEVLMESALLMEATEGHWEKALPLARDLA
                     ALNKGHRMARLILGLAEFKEGRHDAALEHVKASSSGPLGELTGTIASAWIRAAKGEAA
                     AAADALGQTRAQEWAQFHIRYHHALLLDVAGRPQDARAAYRRLFEDARSRALRFMLAY
                     GQHLAASGETKQARTLLDDYAKRAGPQAHPEARALAERLKGPAPVGLLIETPVDGLAE
                     LFYGLGAALASDGGVSLGAIYLQLSLYLKPSAPFALTEIAGYYEATKRYEDANDVYER
                     ISKDSSMFGAIQIRKAQNLDRLKKVEEAKTLLEAMAKADPTDIRPLDALGLLMRGHKR
                     YEEAIGYYTKAIALIGRPEKRHWTYFYARGTSYERIKRWPAAEADLLKAMQLAPDQHL
                     VLNYLGYTWVDQNRNLKQGLALIEKAVSLKRDDADIVDSLGWAHFRLGNFKDAAKHLE
                     RAVELRPEDPTLNDHFGDALWRVGREREARFQWGLALSLNPEPQDAEKIKLKLEKGLP
                     ALRRTVPRGKDRMTRSDTTKRPIEPRAGLPSRPAPQ"
     gene            3485..4054
                     /locus_tag="PK264_10615"
     CDS             3485..4054
                     /locus_tag="PK264_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD76378.1"
                     /translation="MSILTLVMRDAHARSMKVISRWQRDASGVTAIEFAMVAMPFLML
                     LFGIIGIGLYYFTTFSMENAVEQAARLIRTGQAQQTGMNAAQFKAEICSRAPGFIDCT
                     NKLRVSVKTYNEWTAITPATQPKCLDEGNLITATEYSPGGSGEIVLVWVCLEWDFAAK
                     IPFLNLADMSNGSRLIQVATTFRTEPYQN"
     gene            4072..>4475
                     /locus_tag="PK264_10620"
     CDS             4072..>4475
                     /locus_tag="PK264_10620"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD76379.1"
                     /translation="MSILKKARRAHIAFLAKARNLRRSESGIAAIEFAMVVPIMFFLF
                     VGAVEFSQALTVDRRVTQSASATADLVARTDRMTLAAVDNTLKIVEQLIQPYDANALT
                     VAVVSVKASASGGPVPNVTVDWSRNNKGATPYS"
     assembly_gap    4476..4485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4486..4699
                     /locus_tag="PK264_10625"
     CDS             <4486..4699
                     /locus_tag="PK264_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76380.1"
                     /translation="AGAAYPHVPAGLLVAGEWVIVGEAVYNYTPLIFNYFITSAFNME
                     ERFFLKPRQKSCVSLEPINCATGNPF"
     gene            complement(4832..5791)
                     /gene="nudC"
                     /locus_tag="PK264_10630"
     CDS             complement(4832..5791)
                     /gene="nudC"
                     /locus_tag="PK264_10630"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HRD76381.1"
                     /translation="MPTVATTTLLDRMGPKRSDKDWLAGQRSSSAARFMVFTDLKPVI
                     NGSADRSSASIRWLSTSEIADLGLQTEDAMFLGLSRDAAVPHFAIAMTEHFARTVRGG
                     VEHLRPAVDLRSLAMQGLLTPEEQSLAGQARALAQWHENARCCGRCGSQTRVKDGGWR
                     RKCWSCGTDWFPRTDPVVIMLVTDGTRCLLAHEHRYQNSMWSTLAGFLEPGEDIEHAV
                     RREVHEETGIRVGKVAYHSSQPWPFPHSLMMGCIGFAETTELKIDKSELADARWFSLD
                     EVRSMAERRHPEGLTVPGKHAIASILIRSFLEGKVEAMRSTAR"
     gene            6002..7102
                     /locus_tag="PK264_10635"
     CDS             6002..7102
                     /locus_tag="PK264_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD76382.1"
                     /translation="MKRAFKAAAVAAAALAVVGMSGASAQQPKVLKMQASWPASLTLY
                     ENFTQFAKRVEQLTAGRIKIETMPAGQVVPPFEVLEATHKKVIDGHHSWAGYWVGKNK
                     AAILFSGGPGGPLGMDHMDYMGWMWHGGGDALYNKFYTETLKLNVVVISILPSAPQAL
                     GWFKKKIENLADFKGQKCRQTGVAAEIFNEMGMRTVNMAGGEIMPAAERGTIDCAEWV
                     GGIEDLRFGFQKIWKWHYAPSLHESVSVGELVINGDAWKSLSAMDQEIIRAVAVETMF
                     TWWAKWQRQNADALKELRDTHKVNIELTPPEIMTEFLAAWDRIAAREAEKNPVFKTIW
                     ESQKAWASVVVPAKAFYYPAYSLGANHYWGKK"
     assembly_gap    7124..7140
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7284..7703
                     /locus_tag="PK264_10640"
     CDS             7284..7703
                     /locus_tag="PK264_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD76383.1"
                     /translation="MANSDTIRPSAAYLGVIHFIDTITDWSSKLFMLLIVPLIFANVV
                     EVVSRYFFGDPTVWALDMTTMAFGAMFMLGSAFALLKGAHVRTDMLWDKFSERKKGII
                     DSCAYIFFFLPSMAAIAYIGWEGFAYSYSLGEYQREV"
     gene            7660..7860
                     /locus_tag="PK264_10645"
     CDS             7660..7860
                     /locus_tag="PK264_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76384.1"
                     /translation="MPTPTRSANINEKSNSGAWQPIIWPLRGAIPLTAALLFFQGISE
                     LMKSLWAARTGQVLVKHDKIEV"
     assembly_gap    7981..8036
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8037..9574
                     /locus_tag="PK264_10650"
     CDS             <8037..9574
                     /locus_tag="PK264_10650"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD76385.1"
                     /translation="TMKSDILPAVPLFIFMGYMTEQAGLMERLFVAMRSIMAPVRGAL
                     YLAVILTATIFAMATGIVGAAVTVLGIMAGPIMIKSGYDARLSSGAIAAGGTLGILIP
                     PSVMLVVMGPTMGVPVNLLYKASIGPGLLLAGMYIAYCLIRSFINPSLGPPMSPEERR
                     VADDTMMNEQVSLPVRTLGFAATVLLAPAVVALVTMQFLGWEAPIAIVKNKFVYDPKG
                     LLVLVLLVVSAIGAGAGYIYNAYFRAICIGIAPLAALIATTLGTIVGGLATPTEAASC
                     GAFGASLLALVYGKLTLGDTSRPLFVAASLAAAVVGGIIGWTYVGGVSGTAFGAIFGG
                     TLLGFILSPPLYNAGMGTMTTSAMVLFLAVASNIFGAVFAKLGTASVITNGLIALPLP
                     DLGKLALIMLIIFLLGWPFEWPAIILVFLPIFLPVFDKLQIAGLTKLEMLLWFGALVA
                     VNLQTAFLSPPVAMSAYYLRNVVPQWSLATIYRGMADYMVIQIICLLLLFFFPALALF
                     GVR"
     gene            9959..10381
                     /gene="ndk"
                     /locus_tag="PK264_10655"
     CDS             9959..10381
                     /gene="ndk"
                     /locus_tag="PK264_10655"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRD76386.1"
                     /translation="MAIQRTFSIIKPDATRRNLTGSINAVIEKSGLRVVAQKRVRWSL
                     EHAQKFYEEHKARPFYGELCTFMTSAPIVVQVLEGEDAIQNYRDIMGATDPKEAKDGT
                     IRKLFAVSKGENSTHGSDSPASAAREIALNFAEFEIVG"
     gene            complement(10560..>10999)
                     /locus_tag="PK264_10660"
     CDS             complement(10560..>10999)
                     /locus_tag="PK264_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRD76387.1"
                     /translation="ADALTALAPLGSRTFPAERVILTPHEGEFRRLFPEAAGSKLDCA
                     RQAAERSGAVVLLKGADTVIAAPDGCAAINENAPPWLATAGSGDVLAGIITGLAALGL
                     PTFEAAAAGAWLHGRCGALAGPALIAEDLASHIGAALVAGGHP"
     assembly_gap    11000..11019
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11020..>11378)
                     /locus_tag="PK264_10665"
     CDS             complement(<11020..>11378)
                     /locus_tag="PK264_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRD76388.1"
                     /translation="FANHPGLWGGAYRSAARGLASHKYGHGHAIVVSGPAESASAARL
                     AAVAALRAGAGLVTLATPRAAFPVNAAHMTSVMVAPFDVPDGLTKVLADPRRNAVLLG
                     PGGGTGEAMRRMVEIALA"
     assembly_gap    11379..11430
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11431..11992)
                     /locus_tag="PK264_10670"
                     /pseudo
     CDS             complement(11431..11992)
                     /locus_tag="PK264_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
     gene            complement(12038..12691)
                     /locus_tag="PK264_10675"
     CDS             complement(12038..12691)
                     /locus_tag="PK264_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76389.1"
                     /translation="MRHMIVRSIRCGAPLALLVLALFSGAPAAAQLARHNILVIEHDG
                     GQGAVQRGYGIIEDVTATLKRELNARFGVYDITAFSNVFQLQTFRRQGYDVPPAEVHQ
                     LTTTIKTPPIDMLLTYKVLLVFPTPPAPVPGMPHPGLRPGQPAADTMPVLRVTATLIR
                     TPSGEMVAPIQLPDMVMRNLTTPCLRDQRCLTATLKAAAEPIGVALSNEVADRLASN"
     gene            complement(12965..14134)
                     /locus_tag="PK264_10680"
     CDS             complement(12965..14134)
                     /locus_tag="PK264_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD76390.1"
                     /translation="MDHAVAQRVGHEDCVPTGNPNVAWTDEDYRRCHSETNPDIIIKG
                     CNCMLGTSGLNSGPGTWIGGELTRFKYESMYLFRRGMAYLEIGNLELAHRDVGEALRM
                     VSRRSEDPNSEPWRLSLVEAKAGFERVLAAQRDQVAKLAEREARQRAESARLAKLAEE
                     AQALELLRKEAELKERIAREKAAALELKRRHEMHAADEARKSAAVASSAGAPVSARPL
                     QPILDPSRVGRRVALLIGNARYANAPPLVNPPNDVRAVAAALRRLGFSDVVERHDQTL
                     AGFKEALKSFGDTATNADWALIYFAGHGIQVGQVNYVLPTDARLSRVGHVEDEAVALQ
                     SLLSKVEEARGMRMVIIDACRENPFLPRSRLCALARARTGAGRSSKRYPGRVLGA"
BASE COUNT         2383 a         4742 c         4668 g         2565 t
ORIGIN      
        1 cgatggtgga atgagggagc gcaacgtaga acggcacccc cgtatccttg gcggcgagcg
       61 ctttcaggta ggtgccgatc ttgttggcga cgtcgccgcg gcgcgtgact cgatccgtgc
      121 cgacgatgac gaggtcgact tcgccgcgct gcatgtagtg cccgccggca ttgtcgacga
      181 tgacgtgata gggcacgccg tggctgccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnntt cgtccaccca tacatgcacg ggcagcccct cgtcctgcgc cagatagatg
      301 ggtgcggtcg cggtgccgta atcgacagtg gcgagccagc cgggattgca gtgggtgagg
      361 atgttgacgg gctttccggc cttgcgcttg gcggcggcct tgatgagacc gaggccgtgc
      421 tcgccgatac gccggcaggt ctcgacgtcg tcgtcgcaga tggccgcggc cttcaaccag
      481 gcggcattcg cgcgggccgt cggggcgagc ggcgcgacgg ccttgcgcat ctcatcgagc
      541 gcccagcgca ggttgacggc ggtcggccgc tggctggcga gatgctcgat tgacgcctcg
      601 agggcccggt cggaggggtc acgacgcaag gccatcgcga ggccataggc cgctgtcgcg
      661 ccgatcagag gcgcgccgcg cacctccatg gtcttgatgg cgcgtgcagc ctcgtcggcg
      721 gtggcgagca ccttggtgac gaactggtgc gggagcctcg tctgatcgat gatctcgacg
      781 gcgcggccgt cggctgtcgg ccagatcgtc cggtaatgtc ggccggcaat ccgcatcagt
      841 ggccctcaaa ggccatcagc gccttgctct cgacgccgag ccgggcgagc cgcttcacgc
      901 cgccgagctc ggggaggtcg atcacgaacg aggcgccgag cacggtgccg ccggcgcgct
      961 ggaccagctt gacggcggcc tcggcggtgc cgcccgtcgc gatcaggtca tcgacgatca
     1021 gtatgcgcgc gcccttgctg atggcgtcga catgaatctc gatcgtgtcc tcgccgtact
     1081 cgagctggta gctctgcgag atcgtcttcc agggcagctt gcccttcttg cggaccggaa
     1141 tgaagccgca gccgaggcgg tcggcgactg ctccgccgag gatgaagccg cgcgcctcga
     1201 tgccggcgac ggcatcgacg ggctccgttc gataagactc ggccatacgc gcgatcgctg
     1261 ccttgaagcc ttgcggatcg gcgagcagcg tcgtcacgtc gcggaacatg atgccgggct
     1321 tggggtagtc cggaatggtc cggacgaggc tcctgagatc ctgcatctgg cgaacctcta
     1381 caatggtttt tcgggtgatg gcggcgagca gcgttactgc ggcgcggggc gcgacggcag
     1441 tcccgcgcga ggctcgatgg gtcgcttcgt ggtatcgctg cgggtcatgc gatccttccc
     1501 gcgcggcaca gtgcggcgga gcgccggcag acccttctcg agcttcagct tgatcttttc
     1561 ggcgtcctgc ggctcgggat tgagcgacag cgcaagtccc cactggaagc gcgcctcgcg
     1621 ttctcggccg acccgccaga gcgcatcgcc gaagtgatcg ttgagcgtcg ggtcctcggg
     1681 cctgagctcc acggcgcgct cgaggtgctt tgccgcgtcc ttgaagttgc caagtcggaa
     1741 atgggcccag ccgaggctgt cgacgatgtc ggcgtcgtcg cgcttcaggc tcacggcctt
     1801 ttcgatgagg gcgaggccct gcttcaagtt gcggttctgg tccacccagg tgtagccgag
     1861 gtaattgagg acgaggtgct ggtcgggcgc aagttgcatc gccttcagca gatcggcctc
     1921 tgccgccggc caacgcttga tgcgctcata ggaggtgccg cgcgcgtaga aataggtcca
     1981 atgacgcttc tccggccggc caatgagcgc gatcgccttt gtgtagtagc cgatagcctc
     2041 ctcgtatcgc ttgtggccgc gcatgagcag cccaagcgcg tcgagcggcc gaatgtcagt
     2101 cggatcggcc ttggccatcg cttcgaggag tgtcttcgcc tcctcgacct tcttcaggcg
     2161 atcgagattc tgcgccttgc ggatctggat ggccccgaac atggagctgt ccttggaaat
     2221 gcgctcgtag acgtcgtttg cgtcctcgta gcgcttggtg gcctcatagt agccggcgat
     2281 ctcagtgagc gcgaagggag cgctgggctt caggtagagc gaaagttgca ggtagatcgc
     2341 accgaggctg accccgccgt cgctggcaag ggctgctccg aggccataga agagctcggc
     2401 gagcccgtcc accggggtct cgatgaggag accgacgggg gccgggccct tgaggcgctc
     2461 ggcgagcgcg cgggcctcag ggtgcgcctg cggaccagcc cgtttggcgt agtcgtcgag
     2521 gagagtgcgc gcctgcttgg tttcgccgga cgcggcgaga tgctgcccgt aggcgagcat
     2581 gaagcggagc gcgcgcgagc gggcatcctc gaacagccgc cgataagccg cgcgggcatc
     2641 ctgcggccgg ccggcgacgt ccagcaacag cgcgtgatgg tagcggatgt ggaactgcgc
     2701 ccactcctgc gcgcgggtct gcccgagggc gtcggccgca gccgcagcct cgcctttggc
     2761 agcacgaatc caggcggagg caatcgtacc cgtcagctcg ccgaggggac cgctcgatga
     2821 ggccttgacg tgctcgaggg cggcatcgtg gcgaccctcc ttgaactcgg cgaggcccag
     2881 gatcagtcgg gccatgcggt gacctttgtt gagggcggcg agatcacggg cgaggggaag
     2941 cgccttttcc caatgcccct ctgtcgcctc catgaggagc gcactctcca tcaggacctc
     3001 gttggcgggg tcctgagcga gggcgcgacg atagagggcc gctgccgcgt cggtctcgtg
     3061 gagcgcgcgg gcgtagcggc ccgccaggta gctgccgagc agcgacttgc tatcggggag
     3121 gtctgcgacc ccgccgcggg caaaggccgt aacagcggtg ccgaccagcg ccaacagggc
     3181 gcagagagcc gatattgcag gttttgaaag cgtgcgcttc acgtcacgtc gcctcgtcgg
     3241 tcacgtcatg ggcctcgagc agctcgggag acgctcgact cgccaccgac cttagcaaag
     3301 gttgcatgag cttggccatc gcgagcgttc ggctctccct acgtgccaat tcgagacggc
     3361 ggatcagtcg atcttcacct tgaggcggac atctggtgcc gccatcgcca ggggtaagct
     3421 gtcatttatg tttctcgcct tttattcaat caaaccagaa gtctgtacgg gcgaggcgtc
     3481 ggtcatgagt atccttacgc tggtaatgcg cgatgcacat gcgcgctcca tgaaagtgat
     3541 ttcgcgctgg caacgcgatg cctcgggcgt gacggccatc gagttcgcaa tggtcgccat
     3601 gccgttcctg atgctgctgt tcggcatcat cggcatcggg ctctactatt tcacgacatt
     3661 ctcgatggag aacgccgtcg agcaggcggc gcgcctcatc cgcacgggcc aggcgcagca
     3721 gactggcatg aatgccgcgc agttcaaggc cgaaatctgc tcgcgcgcgc cgggcttcat
     3781 cgactgcacc aacaagctcc gcgtcagcgt caagacctac aacgagtgga ccgcgatcac
     3841 gccggcgacg cagccgaagt gcctcgacga ggggaacctc atcacggcca ccgagtattc
     3901 tccaggcggc tccggcgaga tcgtgctcgt gtgggtctgc ctcgagtggg acttcgctgc
     3961 gaagatcccc ttcctgaacc tcgccgacat gtcgaacggc tcgcgcctca tccaggtggc
     4021 gacgacgttc cgcaccgagc cctatcagaa ctgagcgtcg ggagaccggt catgagcatt
     4081 ctcaagaagg cccggcgagc ccatatcgcc ttcctcgcca aggcccgcaa tctgaggcgg
     4141 agcgagtccg gcatcgctgc catcgagttc gcgatggtcg tgccgatcat gttcttcctg
     4201 ttcgtcggcg ccgtcgagtt cagccaggca ctgacggtcg accgccgcgt cacgcagtcg
     4261 gccagcgcca cggccgatct cgttgcccgt accgaccgca tgacgcttgc cgccgtcgac
     4321 aacaccttga agatcgtcga gcagctgatc cagccctacg atgccaacgc gctgaccgtt
     4381 gccgtggtct cggtgaaggc ctccgcatcg ggtggcccgg tgccgaacgt gacggtcgac
     4441 tggtcgcgca acaacaaggg tgcgacgccc tattcnnnnn nnnnnggccg gtgctgccta
     4501 tccgcacgtc ccggccggtc tcctggtcgc cggcgagtgg gtgatcgtcg gcgaggccgt
     4561 ctacaattac actccgctca tcttcaatta cttcatcacc tccgccttca acatggagga
     4621 gcgcttcttc ctgaagccgc gccagaagag ttgcgtgagc ctcgagccga tcaactgcgc
     4681 gacaggcaat cccttttgag tcgaagcagc ccagaattca agtgatcgcc cgcccggacc
     4741 ctaaggtccg cggcgggcga tcactttttg acgcgatccc cccccggctc aactggccgg
     4801 acacaaaggt tgtatgtcgc cgcaacgacc ctcagcgcgc tgtcgaccgc atggcctcga
     4861 ccttaccctc gagaaagctc ctgatgagga tgctggcgat ggcgtgcttg ccgggcacgg
     4921 tcaggccttc gggatgccgc cgctcggcca tcgaccgtac ctcgtccagc gagaaccagc
     4981 gcgcatcggc gagctcggac ttgtcgatct tcagctcggt cgtctcggcg aagccaatgc
     5041 agcccatcat cagcgaatgc gggaacggcc agggctggct cgagtgatag gcgaccttgc
     5101 ctaccctgat cccggtttcc tcgtgcacct cacggcgcac cgcatgctcg atatcctcgc
     5161 cgggttcgag gaagcccgcg agcgtcgacc acatgctatt ctggtagcgg tgctcgtggg
     5221 caagcaggca gcgtgtaccg tccgtgacga gcatgatgac gacggggtcg gtgcggggaa
     5281 accaatcggt gccgcacgac cagcacttgc gccgccagcc gccatccttc acgcgcgtct
     5341 gtgagccgca gcggccgcag cagcgggcgt tctcgtgcca ctgcgcgagc gccctcgcct
     5401 gccccgcgag cgattgctcc tcgggcgtca gcaggccttg catggcgagc gagcgcaggt
     5461 cgacggcggg cctcagatgc tcgacaccac ccctcacggt gcgcgcgaag tgctccgtca
     5521 tggcgatcgc gaaatggggc actgccgcgt cgcggctgag ccccaggaac atcgcgtcct
     5581 cggtctgcag gccgagatcc gcaatctcgc tggtggagag ccatcggatc gatgcgctgc
     5641 tgcgatcggc cgagccgttg atcacgggct tcaggtcggt gaacaccatg aatcgggccg
     5701 ccgacgatga ccgctgcccg gcgagccagt ccttgtcgct gcgcttcggc cccatgcggt
     5761 cgagaagcgt cgtcgtggca actgtcggca tctgcgtcca ctcccgcgtc aagcgtgacc
     5821 aaccacccga gctgtcacta gcgtccgggg ctcgctccac gttgcctgta gtgacgaggc
     5881 gtcgcaacag tccaatcgca aaaaagcatc atgcccgccc ggaaaaccgt gctacggtcc
     5941 gccgcccttg ggcccaacct gcaggtgggc gaggagcaag aaaacgtcgt ggaggatact
     6001 gatgaagcgt gcgttcaagg ctgcggccgt cgcagcagcc gcgctggcgg ttgtcggcat
     6061 gtcgggcgcg tcggcccaac agcccaaagt tctgaagatg caggcgtcgt ggcctgcctc
     6121 tctgacgctc tatgagaact tcacccagtt cgcgaagcgc gtcgagcagc tcacagccgg
     6181 tcgaatcaag atcgagacca tgcccgcagg ccaggtcgtg ccgcccttcg aggtgctcga
     6241 ggcgacacac aagaaggtca tcgacggcca tcactcgtgg gccggctact gggtcggcaa
     6301 gaacaaggcc gcgatcctgt tctccggtgg tcccggcggt ccgctcggca tggaccacat
     6361 ggactacatg ggctggatgt ggcacggcgg tggcgatgca ctttacaaca agttctacac
     6421 tgagacgctg aagctcaacg tcgtcgtcat ctcgatcctg ccgtcagcac cgcaggcgct
     6481 cggctggttc aagaagaaga tcgagaacct cgcagacttc aagggccaga agtgccgcca
     6541 gaccggcgtc gcggccgaga tcttcaacga aatgggcatg cgcacggtca acatggccgg
     6601 tggcgaaatc atgccggccg ccgagcgtgg caccatcgat tgcgccgagt gggtcggcgg
     6661 catcgaggac ctgcgcttcg gtttccagaa gatctggaag tggcactatg cgccgtcgct
     6721 ccatgagtcc gtctcggtcg gcgaactcgt gatcaacggt gacgcctgga aaagcctctc
     6781 ggccatggat caggaaatca tccgcgcggt ggccgtcgag accatgttca cctggtgggc
     6841 caagtggcag cgccagaacg ccgatgccct caaggagctg cgggacacgc acaaggtcaa
     6901 catcgagctg acaccgcccg agatcatgac cgagttcctt gccgcctggg atcgcatcgc
     6961 cgcccgcgag gccgagaaga acccggtctt caagacgatc tgggaatcgc agaaggcctg
     7021 ggcctccgtg gtcgtgccgg cgaaggcctt ctactatccg gcctactcgc tcggcgccaa
     7081 ccactactgg ggcaagaagt gagctgaaac gacacgaggg cggnnnnnnn nnnnnnnnnn
     7141 cttctctacg tctcgagggc cgcctcgaga cgaattggaa cgagcattcg ggggaatgtc
     7201 atcgttcacg aattccggcg aaatcgcgcg acaagacgtg ccgcctctgc tgaagaactc
     7261 gtctgcacca cttgggggaa gccatggcca attccgatac gatacgccca tcggcggcgt
     7321 atctcggcgt catccatttc atcgatacga tcaccgactg gtcgtcgaag ctgttcatgc
     7381 tgctgattgt gccgctgatc tttgccaacg tggtcgaggt cgtctcgcgc tacttcttcg
     7441 gtgatccgac ggtctgggcc ctcgacatga cgacgatggc gttcggcgcc atgttcatgc
     7501 tcggctcggc cttcgccctg ctcaagggcg cgcacgtgcg cacggacatg ctctgggaca
     7561 agttttcaga gcgcaagaag ggtataatcg acagctgcgc ctacatcttc tttttcctgc
     7621 cctccatggc ggcgatcgcc tacatcggtt gggaggggtt tgcctactcc tactcgctcg
     7681 gcgaatatca acgagaagtc taactcgggt gcctggcagc cgatcatctg gccgctccgt
     7741 ggcgccattc ccctcaccgc cgccctcctg ttcttccagg gcatctccga gctcatgaag
     7801 agcctttggg ccgcacgcac gggtcaggtt ctcgtcaagc acgacaagat cgaggtctga
     7861 tcagatgaac attctcgcga cgctcggcat ctcgaacgag ctgtggggcg gtctcatgct
     7921 gttgagcatg atcaccgtca tcttcatcgg cttcccgatc tccttcacgc tgctgttcct
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacac
     8041 gatgaagagc gacatcctgc ccgcggttcc gctcttcatc ttcatgggct acatgacgga
     8101 gcaggccggc ctcatggagc gcctgttcgt cgccatgcga tcgatcatgg cgccggtgcg
     8161 tggcgcgctc tatctcgccg tcatcctcac cgcgaccatc ttcgccatgg caaccggtat
     8221 cgtgggcgcg gccgtcaccg tgctcggcat catggccggc ccgatcatga tcaagtcggg
     8281 ctatgacgca cgcctctcct ccggcgccat cgcggcgggt ggcacgctcg gcatcctcat
     8341 tccgccctcg gtcatgctcg tcgtcatggg accgacgatg ggtgtaccgg tgaacctgct
     8401 ctacaaggcc tcgatcgggc ctggcttgct gctcgccggc atgtatatcg cctactgcct
     8461 cattcgcagc ttcataaatc cctcgctcgg tccgccgatg tcgcccgagg agcggcgcgt
     8521 cgcggacgac acaatgatga acgagcaggt gagccttccg gttcgcacgc tcgggttcgc
     8581 tgcaaccgtc ctgctggccc ccgccgtcgt cgcgctggtg acgatgcagt tcctcggctg
     8641 ggaagcaccg atcgcgattg tcaagaacaa gttcgtctac gatccgaagg gcctactcgt
     8701 ccttgttctg ctcgtcgtct ccgcgatcgg cgccggcgcc ggctacatct ataacgccta
     8761 tttccgcgcc atctgcatcg gcatcgcgcc gctggcggcc ctgatcgcca cgacgctcgg
     8821 caccatcgtc ggcggtctgg cgacgccgac cgaggccgcc tcgtgcggcg cttttggcgc
     8881 gagccttctc gccctcgtat acggaaagct cacgttaggc gacacgtcgc ggccgttgtt
     8941 cgtcgcggcg agccttgctg cggcggtcgt cggcggcatc atcggttgga cgtatgtcgg
     9001 cggcgtttcg ggcacggcct tcggcgcgat attcggcggt acgctgctcg gcttcatcct
     9061 gagcccgccg ctctataatg ccggcatggg cacgatgacg acgtcggcga tggtgctctt
     9121 cctcgccgtc gcctcgaaca tctttggcgc cgtgttcgcc aagctcggca cggcctccgt
     9181 gatcaccaac gggctcatcg cactgccatt gcccgatctc ggcaagctcg cgctgatcat
     9241 gctcatcatc ttcctgctcg gctggccgtt cgagtggccg gcgatcatcc tcgtcttcct
     9301 gccgatcttc ctgcccgtgt tcgacaagtt gcagatcgca ggcctcacga agctcgagat
     9361 gctgctctgg ttcggcgcgc tcgtcgctgt gaacctgcag acggccttcc tcagtccacc
     9421 cgtcgccatg tcggcctact acctccgaaa cgtcgtaccg cagtggtcgc tcgcaacaat
     9481 ctatcgcggc atggcggact acatggtcat ccagatcatc tgcctgctgc tccttttctt
     9541 cttcccggcc ctcgccctct tcggggtacg ctgaggcgaa gcccagagag ttgcagctcg
     9601 ctgtaaatgt ccgggccgcg gcggggcaat cccctccgcg gcccgtcccg cttcaggtcg
     9661 caggccacgc cgaccatggc agccactttc attttgccga acgtgttcgc ttcgcgagaa
     9721 gggctgccgc ccttcaaccc gctcggggag accccgaacc ctcttccttt tggctttcaa
     9781 agaagggtgg gtttggaagg tcccctcccg agcaggtctg ggcggcagcc cagtcgcgca
     9841 gcgaatacgg cggtcgttga ggttggcttg ctgccatccg accctgccgc tataagcgcg
     9901 ccgagcgtgg cgcgctctgc ccgacgcgac agcttcccat cttcaacgag gaccactcat
     9961 ggcaatccaa cgcaccttct cgatcatcaa gccggacgca acccgccgca atctgaccgg
    10021 ctcgatcaac gccgtcatcg agaagtcggg gctgcgcgtc gtcgcccaga agcgcgtgcg
    10081 ctggtcgctt gagcatgcgc agaagttcta cgaggagcac aaggcgcggc cgttctatgg
    10141 cgagctgtgc acgttcatga cctcggcgcc gatcgtcgtc caggtgctcg agggcgagga
    10201 cgccatccag aattaccgcg acatcatggg cgcgaccgac cccaaggaag ccaaggacgg
    10261 caccatccgc aagctcttcg ccgtctcgaa gggcgagaac tcgacgcacg gctccgacag
    10321 cccggctagc gccgcccgcg agatcgccct caacttcgcc gagttcgaga tcgtcgggtg
    10381 aaggggggag gtcgggaagg tcgggtgtca gacccatggg tctgacaccg tcactcgcaa
    10441 ctgacccggg ccagaccgtg aatgctgagc ctcgctcagc aagtgggctg ccgcccacac
    10501 ccgcccgggg gaggcttccc cccggccccc ctctttgttc ggaccaatac gtgggaaggt
    10561 caggggtgcc ccccggccac aagcgccgct ccgatgtggc tcgcgaggtc ctccgcgatg
    10621 agcgccggtc cggcaagcgc gccgcatctc ccgtgcagcc aggcgcctgc cgccgcggct
    10681 tcgaaggttg gcagccccag cgctgcgagc cccgtgatga tgccggcgag cacgtcgccc
    10741 gacccggcgg tcgcgagcca cggcggcgcg ttctcgttga tcgctgcgca tccatccggc
    10801 gcggcgatta ccgtgtcggc gcccttgagc agcacgacgg cgccggagcg ctccgcggcc
    10861 tgccgcgcgc agtcgagctt ggagcccgca gcctcgggaa agagccgacg gaactcaccc
    10921 tcgtgcgggg tgaggatgac gcgctccgct ggaaacgtgc gcgacccgag cggcgccagc
    10981 gcggtcagcg catccgcatn nnnnnnnnnn nnnnnnnnng caagcgcgat ctcgaccatg
    11041 cgacgcatgg cctcgccagt gccgccgccg gggccgagca gcacggcatt gcggcgcgga
    11101 tcggcgagca ccttggtgag gccgtccggc acgtcgaagg gcgcgaccat cacgctcgtc
    11161 atgtgcgcgg cattgaccgg gaacgccgcg cgtggcgtgg cgagcgtcac aagtccggca
    11221 cctgcccgca aggccgcgac cgccgcaagc cgtgcggcac tcgcgctctc cgctgggccc
    11281 gagacgacaa tcgcgtgacc gtggccgtac ttgtggctcg cgagcccgcg cgccgcactc
    11341 cgataggcac cgccccacag gcccggatga ttggcgaann nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cacgcactgc gccgcagagt ccgcgtcccg
    11461 gcagcagcag gtggccgggc ttacgcctga agaacgtgac ggtttcggtc gcctcgatta
    11521 cggagtcgcc gacgaccgcg cccgaggtgc cgtcgaggcc gctcggcacg tcgaccgcga
    11581 caacgggggc cgcacccgcg ttgcaggcgg cgacgagatc gcgggcgagc ccctcgagcg
    11641 gccgcgcaag gcctgcgccg tagagcgcgt cgatcacggt ctcggcgccg tcgagcatct
    11701 cggtgcgggc gcgctcgtag ttgccccagc gcgccgcgac tgcggcggcg tcgcctttca
    11761 ttgcgcctgc ttcgacgaga cacccgagcg tcacgcgata gccgcgctca cgcagaatgc
    11821 tcccggcgac aaagccgtcg ccgccgttgt tgccgggccc gcagagaatc gtgacgcgcc
    11881 cgccgaccga ggcatgctcg gcagcggcgt ccgcgacggc gagcccggcg cgctccataa
    11941 ggagaagcga gggcgtgccc cgcgacactg cggcacgatc agcttcggcc atctcgcgcg
    12001 tcgtcaggag ctcgaggcca ctcgacacgg gcggtcctca gttcgacgcg agccggtcgg
    12061 cgacctcgtt ggagagtgct actccgatcg gctcggcggc ggccttgagc gtggccgtca
    12121 ggcagcgctg atcgcggagg cacggcgtcg tcagattgcg catcaccatg tcggggagct
    12181 ggatcggcgc caccatctcg ccgctcggcg tgcggatgag cgtcgccgtg acgcgcagga
    12241 cgggcatcgt gtcggcagcc ggttgcccgg gccgtaatcc aggatgcggc atgccaggca
    12301 cgggcgccgg cggcgtcggg aagacgagca aaaccttgta ggtcaacagc atgtcgatcg
    12361 gcggcgtctt gatggtggtc gtcagctggt gcacctcggc cggcggcacg tcatagccct
    12421 gccgcctgaa ggtctggagc tggaacacgt tcgagaacgc cgtgatgtcg tagacgccga
    12481 agcgcgcatt gagctcgcgc ttcaaggtgg cggtcacgtc ctcgatgatg ccgtagccgc
    12541 gctgcaccgc gccctgcccg ccgtcatgct cgatgacgag gatgttgtgg cgggcgagct
    12601 gggcggcggc gggtgcacca ctgaacaatg cgagaacgag gagggccagc ggcgctccgc
    12661 atcggattga gcggacgatc atgtggcgca tgttgtgctc cggtctgcag gcttggggaa
    12721 agtttgatca gtgcttgatc gggggccaag gggtcgaacc tacttcgctg gccggaacga
    12781 gatgctcgtg cccggaagcg acccgtaggt gaacggctcc tgagcaccgt tggtgcgttt
    12841 caggacatta tcccgcactt ttcggaacaa gagaccgata tcgagccccg gctcgtcgat
    12901 ttcgccaagc agagcctctg caaaggggct gagtgcgccg gctccatcct cggcaagctg
    12961 cccgtcacgc gccgagtacg cgaccaggat accgcttgga ggagcgaccg gcgccagtcc
    13021 gcgcccgagc gagcgcgcag agccggctgc gcggcaggaa tgggttttcc cggcaggcat
    13081 cgatgatgac cattcgcatg ccccttgcct cctccacttt ggagagcaaa ctctgaagcg
    13141 ccacggcctc gtcctcgacg tgaccgacgc gggacagtcg cgcgtccgtc ggcaggacgt
    13201 agttgacctg cccgacctgg atgccgtggc cggcgaagta gatcagcgcc caatcggcat
    13261 tggttgcagt gtcgccgaac gatttgaggg cctctttgaa acctgccagt gtctggtcat
    13321 gccgctcgac gacgtcagaa aaacccagtc ggcgcaatgc cgccgcgacc gcgcggacat
    13381 cgttgggcgg attgacgagg ggtggtgcgt tggcatagcg ggcattgccg atgagcaggg
    13441 cgacccggcg gccgacccga ctgggatcca ggatcggctg cagcgggcgt gctgatacag
    13501 gggcaccggc cgaactcgcg accgcggcgc tcttgcgggc ctcgtcggcc gcgtgcatct
    13561 cgtgccgtcg tttgagttca agcgccgccg ccttctcgcg ggcaatccgc tccttcagct
    13621 ccgcctcctt gcgaagcagc tcgagtgctt gcgcctcctc agcgagcttg gcgagccgag
    13681 cgctctcggc acgctgccgg gcctcacgct ccgcgagttt cgcgacttgg tcgcgttgag
    13741 cggcgagaac acgctcgaaa ccggccttcg cctcgacgag gctcagccgc cacggttcag
    13801 agtttggatc ctcggaccgc cgagagacca ttcgcagcgc ctcgcccacg tcacgatgag
    13861 cgagctcgag attgccaatc tcgagatagg ccatgccccg ccggaacaga tacatggact
    13921 catacttgaa gcgcgtgagc tccccgccga tccaggtacc gggaccggag ttcaatccgc
    13981 tcgtcccgag catgcaattg cagcccttga tgatgatgtc ggggttggtt tccgaatggc
    14041 agcgccggta atcctcgtcg gtccatgcca cgttggggtt cccggtcgga acgcaatcct
    14101 catgcccgac gcgctgggca acggcgtggt ccatgcccga cgccaccgcc aaaaccatcg
    14161 tggctgccag caggcgctgt gctaccgatg aagccatcat accaaagagc gagcgcttcc
    14221 gaagccttcg ggttggccat tcaatcggcg cgaacatggc gagttcctgg tgttgcatgc
    14281 cagcgtgttt ccgcaggagc gtgccagaag cgcgccagcg gaaccagccc atgcatacag
    14341 ggctgaaaca tgagaggcca acttcgcgga gcacccacga caaagggcac caggatctcg
    14401 cctgatgccc ttgaagaccg gagagcccgc gcggacctca gcccttcttg cccttcatcg
    14461 ccttcagcac cttgggcggt gacatcggca gctcgaaaaa gcggatgccg gtggcgccct
    14521 tcatggcgtt ggcgatcgct cccatgggcg gc
//
LOCUS       DAORNH010000087        14505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3153_length_14505_cov_2.626920, whole genome shotgun sequence.
ACCESSION   DAORNH010000087 DAORNH010000000
VERSION     DAORNH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14505
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3153_length_14505_cov_2.626920"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="nuoL"
                     /locus_tag="PK264_10685"
     CDS             complement(<1..1334)
                     /gene="nuoL"
                     /locus_tag="PK264_10685"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRD76391.1"
                     /translation="MIYQAIVFLPLLGALIAGLFGRAIGDRASEIITSTFLVIAATLS
                     WVVLVQVGLGHTTAKVRIMSWVVSGELDVSWGIRVDTLTAVMLVVVNTVSALVHIYSI
                     GYMHEDESRARFFSYLSLFTFAMLMLVTSDNFLQLFFGWECVGLASYLLIGFWYHKPE
                     ANAAAIKAFVVNRVGDFGFALGIFGLFFVFGSISFDTVFEAVKTYAAKPAGERTIAFL
                     GYQVDTLTCLCLLLFLGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMV
                     ARLSPIFEFAPVALQVVTLVGTVTAFFAATVGLVQNDIKRVIAYSTCSQLGYMFAACG
                     MGAYAAGIFHLFTHAFFKALLFLGAGSVIHAMHHEQDMRYMGGLREKIPVTWAVMLIG
                     TLALTGVGIPHLIGFAGFHSKDAIIEAAYAAATPGNYVFWMLVAAAFMTSFYS"
     gene            complement(1510..1818)
                     /gene="nuoK"
                     /locus_tag="PK264_10690"
     CDS             complement(1510..1818)
                     /gene="nuoK"
                     /locus_tag="PK264_10690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRD76392.1"
                     /translation="MTIGLGHYLTVAAILFTLGILCIFLNRKNVIVILMSVELMLLAV
                     NINLVAFSSFMGDLVGQVFALFVLTVAAAEAAIGLAIVVVYFRNRGSIAVEDINMMKG
                     "
     gene            complement(2150..>2662)
                     /locus_tag="PK264_10695"
     CDS             complement(2150..>2662)
                     /locus_tag="PK264_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76393.1"
                     /translation="GPNPAVAWRPSRERVKPISGATRVGYEDSGDNDFNDAVTVFRVA
                     PPSGLSVTGTRVCVPAGGLVAFMVTFSAAWENGIKIVNERSGQPVRTFNNYFGRGAGG
                     EWNQGAGVWRTRVAGPTCFQVLGQHKTSPPNPAGAWIPSRHRTTGTRIGFEDAGDNDF
                     NDAVVVVSPN"
     assembly_gap    2663..2739
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2740..2950)
                     /locus_tag="PK264_10700"
     CDS             complement(<2740..2950)
                     /locus_tag="PK264_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76394.1"
                     /translation="MKLKLLAAAVVLAAGLGEAQAHSLCVPVGSRIDFDVSFNAAWEN
                     AVIISSSSGARLRTFNNAFGKGAGGE"
     gene            complement(3097..3528)
                     /locus_tag="PK264_10705"
     CDS             complement(3097..3528)
                     /locus_tag="PK264_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76395.1"
                     /translation="MRLVDITLKVLGISEAIRKWQSVARDLDGNRRDRIARYAEEIAG
                     TIARIADAMGRLAELPGDRASLMVITRELGRLSGYIETIVACLEGRIDGRGLNGVKRR
                     LESLREGAVMGTAAQPVDAGQIDRLVAAEGYFRALADSLRT"
     gene            complement(3594..4358)
                     /locus_tag="PK264_10710"
     CDS             complement(3594..4358)
                     /locus_tag="PK264_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRD76396.1"
                     /translation="MSRATILQIIRYPVKGLSAEPLERVTVTAGETLPGDRIYAIENG
                     PGRFDPEAPRHLPKVSFLMRARNGRLATLSTRFDPATHVLTIERGGRPVASGDLSSPI
                     GRRMIEQFMAAFMQGELRGAPKVVSAAGHSFTDVAVKCLHIVNLASVRDLERVSGRAI
                     DPLRFRANIYIDGLAPWEEFRWLDMDLAVGTARLSVIDRTHRCAATEVDPASGERNVA
                     VTSELRRHFGHEDFGIYAKVITGGEIGVGDGLGVVG"
     assembly_gap    4545..4590
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4591..5054
                     /locus_tag="PK264_10715"
     CDS             <4591..5054
                     /locus_tag="PK264_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD76397.1"
                     /translation="IVYRRGVPVAGVAQPEAVIDRLFARNGWGNSWVNGIYNFQHYHA
                     TVHEVLGIARGRGSVQFGGPEGRILEIEAGDVVIIPAGVGHCRLASKGLSVVGAYPQG
                     SSSIDLRRANAADLAGAAELIARVPLPPSDPVHGRDGPLPKIWRSILSAPP"
     gene            5127..6194
                     /gene="rfbB"
                     /locus_tag="PK264_10720"
     CDS             5127..6194
                     /gene="rfbB"
                     /locus_tag="PK264_10720"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRD76398.1"
                     /translation="MRVFVTGGAGFIGSAVCRHLVSETEASVLNVDKLTYAANLTSLA
                     SIATSPRYAFRHGDICNRRLIDALLDAFEPTAIIHLAAESHVDRSISGPAAFIETNIV
                     GTYTLLEATRRYWSELPKAKQERFRFLHVSTDEVYGSLGPTGLFTEETAFDPSSPYSA
                     SKAASDHLVMAWHRTYGLPAMISNCSNNYGPYQFPEKLIPLMILNALDGKALPVYGKG
                     DNVRDWLHVEDHARALALIVAKGRPGETYNVGGRNEATNLEVVERIAELMDKFRPEAA
                     PHRNLIRYVADRPGHDHRYAIDATKLESELGWRAEETFRTGLARTVRWYLANQKWWQP
                     LRERVYSGERLGLGAEQRAAG"
     gene            6465..6734
                     /locus_tag="PK264_10725"
     CDS             6465..6734
                     /locus_tag="PK264_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76399.1"
                     /translation="MRYFALSAAAIAAGLSMLGPTASTPAEAAHCRYKGVTVEGRALD
                     WVQGNGYAFKQDNACDRARRECHRDLERHYRRGDLPRGVVCKRTA"
     gene            complement(6789..7703)
                     /locus_tag="PK264_10730"
     CDS             complement(6789..7703)
                     /locus_tag="PK264_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRD76400.1"
                     /translation="MRIFCTGASGYIGGSVGVALVAAGHVVSGLVRSEASAVAVAAVG
                     IAPVRGTLDDLDMLRAEATRADAVLNAASADHEPSALAMLEALAGTGKAFVHTSCSSI
                     VGSAAEGRAVDDVFDEATPWTPSPGRAARVALNRRILGYAQKNVRAVIVCPSLIYGPG
                     TGPGKESIQVPWLIALARKSGIARHYGPGENIWSNVHIADLVDLYRLALERAPSGAFY
                     FAENGECSMKEVCRAINRRLGIPGEPVTLSREEALREWGEGATDNTMGSNSRVRAVRA
                     RNELDWRPHRPSLVDEIMTGSHAASLVC"
     gene            complement(7800..8861)
                     /locus_tag="PK264_10735"
                     /pseudo
     CDS             complement(7800..8861)
                     /locus_tag="PK264_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
     gene            complement(8921..>9560)
                     /locus_tag="PK264_10740"
     CDS             complement(8921..>9560)
                     /locus_tag="PK264_10740"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRD76401.1"
                     /translation="DYWAAGVRHIVALRGDMPAGPGIKFEPHPGGYKNAADLVAGLRR
                     LAKFEISVAAYPEKHPDSPSVAADIDNLKAKIDAGANRAITQFFFDNAHYLRYMEKVH
                     AAGIKVPVVPGIVPVHNFKQVASFSARTGASVPDWLARRFDGLENDPVTAPLIAAAVA
                     AEQVMDLVDHGIRQFHIYTLNRADLAYAICHLLGIRPVATANDGATARGNAA"
     assembly_gap    9561..9580
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9581..9898)
                     /locus_tag="PK264_10745"
     CDS             complement(<9581..9898)
                     /locus_tag="PK264_10745"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HRD76402.1"
                     /translation="MITFPEGSSSTKPAADRSPAERKSRLLGNGQIEVSFEFFPPKNV
                     KMEEALWSAIRRLEPMHPNFVSVTYGAGGSTRERTHATVSRIVKETMVVPAAHLTCVQ
                     ATKG"
     gene            complement(9895..10926)
                     /locus_tag="PK264_10750"
     CDS             complement(9895..10926)
                     /locus_tag="PK264_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD76403.1"
                     /translation="MTTPPRLAPPTMLEALKAAADPTRLRLLALLAGGELNVSDLTTI
                     VGQSQPRVSRHLKILAEAGLIERFRDGSWVFLSLASDGPHAAIARSVLALMETESGEL
                     ARDRERLHRLVSERERAAQAYFDTHAAEWDRIRALHVAEGEVEDAMRRALGSGPFKLL
                     VDLGTGTGRMLELFADRYERAVGFDINAAMLAYAQSRLQAAALTHAQVRHGDLRSVRL
                     PDGSASVVVMHQVLHFLADPSVALREAARILAPGGRLLVVDFAPHDLEFLREDQAHVR
                     LGFSDEVMEGWLADCGLETTHRERLIPRGRRKNDAGELTVSLWLAAPVDTTSTPAKKR
                     KRNLEATSP"
     gene            complement(11004..11780)
                     /locus_tag="PK264_10755"
     CDS             complement(11004..11780)
                     /locus_tag="PK264_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRD76404.1"
                     /translation="MLPFAESSAAIQGRRSYQEDSCAVAKVAETIGGSTVRLLAVLAD
                     GMGGHAGGARASRTICGTMIASFGAQAEGDDSVATRLLGCLAASNKAIGDIVEAEAGL
                     AGMGATVVGVAVEDDRLHWISVGDSPLYLVRGGEIARLNEDHSLAPMLDKMAAEGRIS
                     REAAASDPRRHYLRSAVTGEELDLVDQPAKPLPLEAGDCILVMSDGIHTLEESEIAAI
                     VAAEQPAGPERIVARLLASVEAAHDTHQDNTTVIAIVFGG"
     gene            complement(11780..12325)
                     /locus_tag="PK264_10760"
     CDS             complement(11780..12325)
                     /locus_tag="PK264_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRD76405.1"
                     /translation="MTNKKEPEPRPSAAEAAREARGARPAEDVTTRVLRASAPAVPAR
                     TQLIRGKIAVKRTEFHQDPVVGWLVVVGGPGLGAYRPVFEGNNTLGRASSQRVPIDFG
                     DDTISSEEQAYIRYDGSERKFLLVPNLAKTNVVSVNDKRPTAAVELAAMDLITIGRTQ
                     LVFMPFCGPDFDWSELGELKS"
     gene            complement(12709..13335)
                     /locus_tag="PK264_10765"
     CDS             complement(12709..13335)
                     /locus_tag="PK264_10765"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRD76406.1"
                     /translation="MVFQTARLVLGTCLFLGLAALGAAIVLSPELGVVAEELALEAWI
                     GARATVSAAYAKSPAMVISLSVALALPVAAVSADIAHRMTRRRQLRGASLRRSPRGDI
                     LTSRVPRGAWIEVDGEQGRRHLIEREILSIGREIDCDVTLDDPALAETHLVIRRDVDG
                     RFLAVDLSGQGIGINGKLQHRAEIGDGDRISLGACRLTMVVSAQPQGR"
     gene            13665..>14505
                     /locus_tag="PK264_10770"
     CDS             13665..>14505
                     /locus_tag="PK264_10770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRD76407.1"
                     /translation="MSNLLALRNGTELAGEYRIERVLGAGGFGITYLAEETSLARLVT
                     IKEYFPSDFAARDGSVDAMPRSLGCAGDYKWGLDRFIAEAQTLARFEHPNIVRVYRYF
                     RANNTGYIVLRYEDGQSLKDWLKSLGRAPRQPELDRILGPLLDALETMHAADYLHRDI
                     APDNIMIRKDGSPVLIDFGSARGEIAQHSKTLSALVKPGYSPYEQYATTGRQQGPWSD
                     IYSLGATLYQAVTGKRPPDAPTRVLGDELKPARDGALSAYRPKFLEGIDRALSLDVEA
                     RPQS"
BASE COUNT         2438 a         4692 c         4858 g         2374 t
ORIGIN      
        1 gagtagaacg acgtcatgaa ggcagcggcg accagcatcc agaacacgta gttgcccggc
       61 gtcgcggcgg cataagcggc ctcgatgatc gcgtccttcg aatggaagcc ggcaaagccg
      121 atgagatggg ggatgccgac gccggtcaat gccagcgtgc ctatcagcat caccgcccag
      181 gtgaccggga tcttctcgcg gagcccgccc atgtagcgca tgtcctgctc atggtgcatc
      241 gcgtggatga cggagcccgc gccgaggaac agcaacgcct tgaagaaggc atgcgtgaag
      301 aggtggaaga taccggcggc ataggcgccc atgccgcagg ccgcaaacat gtagccgagc
      361 tgcgagcagg tcgaataggc gatcacgcgc ttgatgtcgt tctggacgag gccgacagtc
      421 gccgcgaaga aggcggttac cgtgccgacg agcgtcacga cctgcagcgc gacgggcgca
      481 aactcgaaga tcggcgacag gcgcgcgacc atgaacacgc cggccgtcac catggtcgcc
      541 gcatggatga gagcggagac aggcgtcggg ccctccatgg cgtccggcag ccaggtgtgc
      601 aggaggaact gcgccgactt acccatggcg ccgaggaaca gcagcaggca gaggcaggtg
      661 agcgtgtcca cctggtagcc gaggaaggcg atggtgcgct cgccggccgg cttggcggca
      721 taggtcttca cggcctcgaa gacggtgtcg aagctgattg agccgaacac gaagaagagg
      781 ccgaagatgc cgagcgcgaa gccgaagtcg ccgacccggt tgacgacgaa ggccttgatc
      841 gcggccgcgt tcgcctcggg cttgtgatac cagaagccga tcagcaggta gctcgcgagg
      901 ccgacgcatt cccaaccgaa gaagagctgc aggaagttgt cgctcgtcac cagcatcagc
      961 atcgcgaaag tgaagagcga gaggtaggag aagaagcgcg cccggctctc gtcctcgtgc
     1021 atgtagccga tcgagtagat gtggaccagc gctgacacgg tgttgacgac gaccagcatc
     1081 acggccgtga gcgtgtcgac cctgatcccc cacgagacat cgagctcgcc agagaccacc
     1141 cacgacatga tgcggacttt tgccgtcgtg tgcccgagtc cgacctggac cagcaccacc
     1201 cacgacagcg tagctgcaat gaccaggaac gtcgaggtga tgatctcgct cgcacggtca
     1261 ccaatggcgc gcccgaagag gccggcgatc aaggcaccga ggaggggaag gaagacgatg
     1321 gcttggtaga tcatgaggat gcctggagac gtggggcgca gtgctcttgg tgcttagact
     1381 gtggagcttg tcggagcatg tcggaggagt gctgtggagg gtcagcagaa acgtcgtgtt
     1441 cgattgatca ccgagtgttc cgcaccacga accgcgtagt cggttcccag cactcgcccc
     1501 actgctgtct caccctttca tcatgttgat atcctcgacc gcgatcgagc cacggttgcg
     1561 gaagtagacg acgacgatgg cgaggccgat ggcggcttcc gcggcggcaa ctgtcagcac
     1621 gaagagtgcg aacacctggc cgacgaggtc gcccatgaag ctcgagaagg cgacgaggtt
     1681 gatgttcacg gcgagcagca tgagctcgac cgacatcaag atgacgatca cattcttgcg
     1741 gttgagaaag atgcagagaa tgccgagcgt gaacaggatc gccgcaacgg tcagatagtg
     1801 gccaagcccg atggtcatgt gcgcacgccc ctcccccaga gcctcgcgag agcctcgctc
     1861 ggaggcatcg cccgctgact acgaaaaatc gttggtcctg tccatgctcg atgttgcgag
     1921 gcaggagggc gatctggttt gactttggac gggtgcgaag aggccgatca gaccgagatc
     1981 ggcagccact caagctgcga cttcggttcg gtcgcgcggt aggtactcag gccgcacgat
     2041 tgtgcgtcag gcagcattcg tgtgctcccc ctcggccgct acggttgcgc gagccgataa
     2101 tcggctatgc cgacgcgggt cttgcggacc cgcgccgcaa cacaacccgt caattgggtg
     2161 acaccacgac gaccgcatcg ttgaagtcgt tatctccagc gtcctcgaag ccgatgcggg
     2221 tgccggtggt gcgatgccgg ctcgggatcc aggcgcccgc agggttgggc gggctcgtct
     2281 tgtgttgtcc caagacctgg aagcaggtcg gcccggcgac acgcgtccgc cagacgcccg
     2341 cgccctggtt ccattcgccg ccggcaccgc ggccgaaata gttgttgaac gtgcggaccg
     2401 gctgcccgga cctctcgttg acgatcttga tgccgttctc ccaggccgcc gagaacgtca
     2461 ccatgaaggc gacgaggccg cccgccggca cgcagacccg cgtgccggtc accgacaggc
     2521 ccgaaggcgg agcgacgcga aacacggtga ccgcgtcgtt gaaatcgttg tctccgctat
     2581 cctcatagcc gactcgcgtc gcgcccgaga ttggcttgac gcgctcccgg ctcggccgcc
     2641 aagcgacggc ggggttgggc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna ttcgccgcca gcgcccttgc
     2761 cgaaggcgtt gttgaacgtg cgcagccggg cgccgctgct ggaggagatg ataaccgcgt
     2821 tttcccaggc agcgttgaag ctcacgtcga agtcgatcct cgacccgacg ggcacgcaca
     2881 gactgtgagc ctgggcctca ccaagtccgg cggcgagcac cacggcagca gcaagaagct
     2941 tcaatttcat tcgcatggct cctcgaaatc caaccggctg gccgatcagg caaacgcatc
     3001 caacgaaatg ctatcgggcg tggtctgcac ttacaagctg aatgagctgc gcgcgatcca
     3061 tcggccaaag gacgggcggg cacgcgggcg ccgcaatcag gttctcagac tgtcggcaag
     3121 ggccctgaag taaccctctg cggcgaccag ccggtcgatc tgtccggcgt cgaccggctg
     3181 cgccgccgtg cccatgacgg cgccttcacg gaggctctcg agccggcgct tgacgccgtt
     3241 gagcccgcgg ccatcgatgc ggccctcgag gcaggcgacg atcgtctcga tatagccgct
     3301 gagccggccg agttcgcgcg tgatcaccat gaggctcgcc cggtcgcccg gcagctcggc
     3361 gagccgcccc atcgcgtccg cgatccgcgc aatggtgccc gcgatctcct cggcgtagcg
     3421 ggcgatacgg tcgcggcggt tgccgtcgag atcgcgggcg accgactgcc atttgcggat
     3481 cgcctcggag atgccgagga ccttgagcgt gatgtcgacg agacgcatgg aaccgttcga
     3541 ggaatccctg cgcccgatgc tacctgcagt gctggtgagc ggggataatc tggtcagccc
     3601 acgacgccaa gcccgtcgcc gacgccgatc tcgccgcctg taatgacctt ggcatagatg
     3661 ccgaagtcct catggccgaa gtggcggcga agctccgatg tgacggccac attgcgctcg
     3721 cccgacgccg gatcgacctc tgtcgcggcg cagcgatgcg tgcgatctat gacggagagg
     3781 cgcgccgtgc ccacggcgag atccatgtcg agccagcgga actcctccca gggcgcgagg
     3841 ccgtcaatgt agatattggc acggaagcgc agcggatcga tggcgcggcc ggacacgcgc
     3901 tcgaggtcgc gcacgcttgc gaggttgacg atgtggaggc atttcaccgc gacgtcggtg
     3961 aagctgtgcc cggccgccga cacgactttc ggcgcgccgc gcagctcgcc ctgcatgaag
     4021 gccgccatga actgctcgat catgcgccgc ccgatcggcg aggagagatc gccgctcgct
     4081 accggccgtc cgccccgctc gatcgtcagt acgtgcgtcg ccggatcgaa gcgcgtcgag
     4141 agcgtcgcga ggcggccgtt gcgcgcgcgc atcagaaacg agaccttggg cagatggcgc
     4201 ggcgcctccg gatcgaatcg gccggggccg ttctcgatcg catagatgcg gtcgccgggg
     4261 agcgtctcgc cggctgtcac ggtgacgcgt tcgaggggct ccgcgctcaa acctttcacc
     4321 ggatagcgga tgatctgcag gattgttgca cgcgacatgt ggggccttgt tcgtcgtcgt
     4381 tggcttggcg atgatgaacc acgcctgcgc ctgcctgcaa tcgccgaatg tgtatcaccg
     4441 agtgcgtcga gccccaacgc gatgcctacc gcatggtagg gagtttgctc ggccgccaag
     4501 acgtggggat ggtcgcgtga cagacgttgg agcgcaggag ccgannnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgatcgtcta ccgccgaggc gttccggtcg
     4621 ccggcgtcgc tcagccggag gcggtcatcg accggctgtt cgcccgcaac ggctggggga
     4681 atagctgggt caacggcatt tacaacttcc agcactacca cgcgaccgtc cacgaggtgc
     4741 tcggcatcgc acgaggccgg gggagcgtcc agttcggcgg ccccgagggc cggatcctcg
     4801 agatcgaggc cggcgatgtc gtgatcatcc cggccggcgt cggccattgc cgcctcgcct
     4861 ccaaggggtt gtccgtggtc ggggcctatc cgcagggaag cagctcgatc gaccttcgtc
     4921 gggcaaacgc ggccgacctc gctggagctg cggagttgat cgcccgggtg ccactccccc
     4981 catccgatcc cgtccacggc cgcgacggac cgctgccgaa gatctggcgc tcgatcctgt
     5041 ccgccccgcc ttaagggatc gctagggcat cggcgctacg gtccagcctc gacacgtgtg
     5101 ctgtgccagt cgggttggag cgttgaatgc gcgtatttgt gaccggggga gcgggcttca
     5161 tcggctcggc cgtctgccgc cacctcgtct cggagaccga ggcctcggtc ctcaatgtcg
     5221 acaagctcac ttatgccgcc aacctcacct cgctcgcctc gatcgcgaca agcccgcgct
     5281 acgccttccg ccatggtgac atctgcaacc gtcgcctgat cgacgcgctc ctcgatgcct
     5341 tcgagccgac cgcgatcatt catctcgccg ccgagagcca tgtcgatcgc tcgatcagcg
     5401 ggccggcggc cttcatcgag acgaacatcg tcggcaccta cacgctgctc gaggcgacgc
     5461 gccgctactg gtcggagctg cccaaggcga agcaggagcg cttccgcttc ctgcacgtct
     5521 cgactgatga ggtgtacggc tcactcggac cgaccggcct cttcaccgaa gagacggcgt
     5581 tcgacccgtc gtcaccctac tcggcctcga aggccgcgtc cgatcacctc gtgatggcct
     5641 ggcaccgcac ttacgggctg ccggcgatga tctcgaactg ctcgaacaac tacgggccct
     5701 atcagttccc ggaaaagctc atcccgctga tgatcctgaa tgcgctcgac ggcaaggcgc
     5761 ttccggtcta cggcaaaggc gacaacgtgc gcgactggct gcacgtcgag gatcacgcgc
     5821 gcgcgctcgc cctgatcgtc gccaagggcc gccccggcga gacctacaat gtcggcggcc
     5881 gcaacgaagc gaccaatctc gaggtcgtcg agcggattgc cgagctcatg gacaagtttc
     5941 gccccgaggc ggcgccgcac cgaaacctga tccgctacgt tgccgaccgg ccagggcacg
     6001 accaccgcta tgcgatcgat gcgacgaagc tcgagagcga actcggctgg cgtgccgaag
     6061 agacgtttcg caccggcctc gcacgcaccg tccgctggta tctcgccaat cagaagtggt
     6121 ggcagccact gcgcgagcga gtctactcag gcgagcggct cggtctcggc gctgaacagc
     6181 gcgccgcggg ttaagccccg acacaagcgt cgctgcgcga gcgggctgcc gaccgcaacc
     6241 cgcttggggg atgctccccc aaaccccctc cttttcagtt tcgacaagca gtgagttcgg
     6301 aacgcttcgc cccgatgggg cgcggggttc atcgacgctt gcgcgcggcg ccccgcaagc
     6361 gaacgagtgg cctatcagct cgttcatttg aaccatttgc ggcctggcag cgacttaccg
     6421 ctcggaactg ctcgattgat tctcaccctg aaggaacatt ccccatgagg tacttcgcac
     6481 tctctgccgc ggcgatcgcc gcaggcctct cgatgctcgg tccgaccgcc tccacgccgg
     6541 ccgaggccgc ccactgccgc tataagggcg tcacggtcga gggccgtgcc ctcgattggg
     6601 tccaaggcaa cggctacgcc ttcaagcagg acaacgcctg cgaccgcgca cgccgcgaat
     6661 gccatcgcga cctcgaacgg cactatcgcc gtggcgacct gccgaggggt gtcgtctgca
     6721 agcggacagc gtagcaacga atgggccggc cggtgtggct tcaccggccg gccctgaact
     6781 cccgagactc agcagacgag gctcgccgca tgcgagccgg tcatgatctc gtcgacgagc
     6841 gacggccgat gcggtcgcca atcgagctcg ttcctggcgc ggacggcacg cacacggctg
     6901 ttcgagccca tcgtgttgtc ggtcgccccc tcgccccact cgcgcaaggc ctcctcgcgc
     6961 gacaaggtga cgggctcgcc agggatcccg agccgccgat tgatcgcccg gcagacctct
     7021 ttcatcgagc actcgccgtt ctcggcgaag tagaaggcac cggacggagc ccgctctagc
     7081 gccaggcgat agaggtcgac gagatcggcg atgtgcacgt tggaccagat gttctcgccc
     7141 ggtccgtagt ggcgggcgat cccgcttttc ctcgcgagcg cgatcagcca ggggacctgg
     7201 atgctctcct tgcccggtcc cgtgccgggc ccgtagatca ggctcgggca gacgatgacg
     7261 gcacggacgt tcttctgcgc atatccgaga atgcgccgat tgagcgcgac acgtgcggct
     7321 cgccctggcg acggcgtcca tggcgtcgcc tcgtcgaaga cgtcgtcgac ggcgcgtccc
     7381 tcggcggccg atccgacgat gctcgagcag ctcgtgtgaa cgaacgcctt gccggtcccg
     7441 gcaagagctt cgagcatggc gagggcagac ggctcgtggt cggcgcttgc cgcgttgagc
     7501 acggcatcgg cgcgtgtcgc ttcggcgcgc aacatgtcga ggtcgtcgag cgtacctctg
     7561 acgggcgcaa tcccaaccgc ggcgaccgct accgcgctcg cctctgagcg gacgagccct
     7621 gaaacgacat gacccgcggc gacgagcgcc actccgaccg agccgccgat gtaacccgat
     7681 gcccccgtgc aaaagatccg catgactggc tctccgtcgt cctcgatcga ttcattcgat
     7741 ctcgaaccat gcaccaaacc gagtcgcggc ggaggcgaat tcgggggcta cccgtgcacg
     7801 aacggctcga ggccggagag gcgcatgcgg cgggcgagcg ccggcagctt tcgcggcggg
     7861 tacttgagga cgtgctcgcg gatgtgggcg atgtgatcgg gcgtcgagcc gcagcagccg
     7921 ccgacgatgt tgacgaggcc gtcgcgcgcc cagggctcga tctggcaggc catgtcgtgc
     7981 ggggtctcgt cgtagccgcc catctcgttc ggcaggcccg cgttgggata ggccgagacg
     8041 agggtgtcgg cgacggcggc gatctcggcg atgtaggggc gcatcagctc ggcgccgagc
     8101 gcacagttga gcccgatcgc gaacggcttc aggtgccgca tggagtacca gaaggcttct
     8161 gccgtctggc cggagagagt ccgtcccgag cgatcggtga tggtgccgga gatcatgatc
     8221 ggcagagtaa cacccagtgc cttgaacaca tcgagcgtcg cgaagcccgc agccttggca
     8281 ttgagcgtgt cgaagatcgt ctcgatcagg atgatgtccg agccgccctc gatcaagccc
     8341 cgcacctgct cggtataggc ggtgacgagc tcgtcgaagg agacattgcg gaagcccgga
     8401 ttattgacgt cgggcgagag cgaggcggtg cggttggtcg ggccgacggc gcccgcgacg
     8461 aagcgcggcc tgtcgggcgt cttctcgttc cacacgtctg cggcctcccg cgcgatgcgg
     8521 gccgcggcga cgttgatctc gtgggcgagg ctctccatgc cgtagtcggc catcgagatc
     8581 gtctgcgcgt tgaacgtgtt cgtctcgatg atgtcggcgc cggccgcgag ataggcgtcg
     8641 tggatctcgc cgatgatctt cggttgcgtc agcacgagga ggtcgttgtt ccctttgacg
     8701 tcgcggttcc aggaatggaa gcgctcgccg cggtaatcgc cctcctcgag cttgtggcgc
     8761 tggatcatgg tgcccattgc gccgtcgatg atcaggatgc gctcgcgggc ggcggcctcg
     8821 agctctttgg tggtgcgggc gatttcggcg gcgttacgca tggtggcttt ccgggcggaa
     8881 gaggaaggct ttcgaggcat cgcgatgact cgctcggcgg tcaggccgcg ttgccgcgtg
     8941 ccgttgcgcc atcattcgcc gttgcaacgg gtctgatccc gagcaggtgg cagatcgcgt
     9001 aggcgaggtc ggcgcggttc agcgtgtaga tgtggaactg ccggatgcca tgatcgacga
     9061 ggtccatcac ctgctcggcc gcaaccgcgg cggctatcag gggcgcggtg acgggatcgt
     9121 tctcgaggcc gtcgaagcgg cgggcgagcc agtcaggcac gctcgcgcct gtgcgcgccg
     9181 agaagctcgc cacttgcttg aagttgtgaa cgggcacgat gcccggcacc accggcacct
     9241 tgatgccggc cgcgtgcacc ttctccatgt agcgcagata gtgtgcatta tcgaagaaga
     9301 actgcgtgat ggcgcggttg gcgcctgcat cgatcttggc cttcaggttg tcgatgtcgg
     9361 cggcgacgct cgggctgtcg ggatgcttct ccgggtaggc cgcgactgag atctcgaact
     9421 tggcgagacg acggaggccc gcgacgagat cggccgcatt cttgtagccg ccgggatgcg
     9481 gttcgaactt gatgccgggg cccgccggca tgtcgccgcg cagcgccacg atgtggcgca
     9541 cgcccgccgc ccagtagtcg nnnnnnnnnn nnnnnnnnnn gcccttggtc gcctgcacgc
     9601 aggtgaggtg cgctgccggc acgaccatgg tctccttgac gatgcgcgag acggttgcgt
     9661 gcgtgcgctc gcgcgtcgag ccgccggcgc cgtaggtcac ggagacgaag ttcggatgca
     9721 tcggctcgag tctgcggatc gcggaccaga gcgcctcctc catcttcacg ttcttaggcg
     9781 gaaagaactc gaacgagacc tcgatctggc cattgccgag gaggcggctc ttgcgctcgg
     9841 cgggcgagcg gtcggcggcc ggcttggtgg aggacgagcc ttcggggaag gtgatcatgg
     9901 tgaggtggcc tccaggttgc gcttgcgctt cttcgcgggc gtggatgtgg tgtcgaccgg
     9961 cgcggcgagc cacaggctca cggtgagttc gcccgcgtca ttctttcggc gaccgcgagg
    10021 gatgagccgc tcgcgatgcg tggtctcgag accgcagtcg gcgagccagc cctccatgac
    10081 ctcgtccgag aacccgaggc ggacatgcgc ctggtcctcg cgcaggaact cgaggtcgtg
    10141 cggggcgaag tcgacgacga gcaggcggcc gcctggggcg aggatgcggg ccgcctcacg
    10201 cagcgccacg ctcgggtcgg cgaggaaatg cagcacctgg tgcatgacga cgacggaggc
    10261 cgagccgtcc ggcaggcgca ccgagcgcag gtcgccatgg cgcacctggg cgtgggtgag
    10321 tgctgctgcc tgcaagcggc tctgcgcata ggcgagcatg gcggcgttga tgtcgaagcc
    10381 gactgcgcgc tcgtagcggt ccgcaaacag ctccagcata cggccggtgc cggtgccgag
    10441 atcgacgagc agcttgaagg gcccactgcc gagcgcgcgg cgcatggcat cctcgacctc
    10501 accctcggcg acatgcaggg cgcggatgcg atcccactcg gcagcgtggg tgtcgaaata
    10561 ggcctgagcc gcccgctcgc gctcactgac gagccggtgg aggcgctccc ggtcgcgcgc
    10621 aagctccccg ctctccgttt ccatgagcgc aaggaccgag cgggcaatgg ctgcgtgcgg
    10681 gccgtcgctc gcgagactca ggaagaccca gctcccatcc cggaagcgct cgatcagccc
    10741 cgcttcggcg agtatcttca ggtgtcggct cacgcgcggc tggctctgtc cgacgatggt
    10801 ggtgaggtcg ctcacgttga gttcgccacc ggcaagcagg gccagcagcc gcagccgggt
    10861 cggatcggcg gcggccttca aggcctcgag catggtcggc ggagcgagcc gcggtggcgt
    10921 ggtcatgcgc cggacaatat aaagatatct ttatatgtca agtgcgccca cgtgagcctc
    10981 acaggaaatc gaagacgctt gcgtcagccg ccgaacacga tggcgatgac ggtcgtgttg
    11041 tcctggtgcg tgtcgtgtgc cgcctcgacg ctcgcgagca ggcgggcgac gatccgctca
    11101 ggccccgcgg gttgctctgc ggcgacgatc gccgcgatct cgctctcctc gagcgtgtgg
    11161 atgccgtcgc tcatgacgag aatgcagtca ccggcctcga gcggcagcgg cttcgccggc
    11221 tggtcgacga gatcgagctc ctcgcccgtc actgcggagc gaagatagtg gcgccgcggg
    11281 tctgatgcgg cagcctccct ggagatccgt ccttctgccg ccatcttgtc gagcatcggc
    11341 gcgagcgagt gatcctcgtt gagccgggcg atctctccgc cgcgcacgag atagagcggg
    11401 ctgtcgccga cgctgatcca gtgcagccgg tcgtcctcga ccgcgacgcc gacgacggtc
    11461 gcacccatcc cggcgagccc cgcctccgcc tcgacgatgt cgccgatggc cttgttcgat
    11521 gccgcgaggc agcccagcag gcgcgtggcg acgctgtcat cgccctcggc ttgcgcaccg
    11581 aacgaggcga tcattgtgcc gcaaatggtg cggctcgcac gggcgccacc ggcgtgcccg
    11641 cccatgccgt cggcaaggac cgcgagaagg cgcaccgtcg agccgccgat cgtctcggcg
    11701 accttggcga cggcgcagga atcctcctga tagctgcgcc gcccctggat ggcagcgctc
    11761 gactcggcga aggggagcat caggatttca gctctccgag ctcggaccag tcgaagtcgg
    11821 gcccacagaa cggcatgaac accagttgcg tccgcccgat cgtgatgagg tccatggcag
    11881 cgagttcgac ggcagcggtc ggccgcttgt cgttcacgga gacgacattc gtcttcgcca
    11941 ggttcggcac cagcaggaat ttgcgctcgg agccgtcgta gcggatgtag gcctgctcct
    12001 cggacgagat cgtatcatcg ccgaagtcga tcggaacccg ctggctcgag gcgcggccaa
    12061 gtgtgttgtt gccttcgaac acgggccggt aggcgccgag cccgggaccg ccgacgacga
    12121 cgagccagcc gaccacggga tcctggtgga actccgtgcg cttgaccgcg atcttgccgc
    12181 ggatgagctg cgtgcgggcg ggcaccgcgg gcgcgctcgc gcggagcacc cgcgtcgtca
    12241 catcctcggc cgggcgtgcg ccccgagcct ccctggcggc ttcagcggcg ctgggacggg
    12301 gctctggctc tttcttgttg gtcacgatca cgggctccgg cttcgttggg atcggtgctg
    12361 gaagtgatgc cttcggatcg gccacgttgg caaccgggat cgcgaccggc tctcccgacg
    12421 ctggtgcggc cggcggtccc ggtggggtcg gtcgtcgctc atcgagaccg gccgcggcga
    12481 gggcatcgcg gagcgacccc atgatgcgat gcgatggccg ctgcgccgga cgctgcggta
    12541 tagccgtgtc tctcggcccg gaatgattgt ccgacatact ctccccctcc agaatagcgc
    12601 cccctcgggc gcatgcgcgc gcctcgagca cgcctcgcca actcacctaa atcgagtgag
    12661 cgtcgagatt tgggcttgaa tgagactgag cgactgtcgc tggagccgtc acctgccttg
    12721 cggctgggcc gacaccacca tcgtgagccg gcacgcgccg agcgagatcc tgtctccgtc
    12781 gccgatctcg gcacggtgtt gaagcttgcc attgatcccg atgccctgcc cgctgagatc
    12841 gacggcgagg aagcggccgt cgacgtcgcg ccgaatgacg agatgcgtct cggcgagggc
    12901 cggatcatcg agtgttacgt cgcaatcgat ctcgcggccg atgctcagga tttcccgctc
    12961 gatgaggtgt cggcgtccct gctcgccgtc aacctcgatc caggcaccgc ggggtacgcg
    13021 agacgtcaga atgtcgccgc gcggggagcg gcgaagagag gcgccgcgca gttgacgccg
    13081 acgcgtcatt cgatgcgcaa tatcggccga aaccgcggcg accggcagcg ccagcgcgac
    13141 cgagagactg atcaccatcg ccggcgactt tgcataggcc gccgagactg tggcgcgcgc
    13201 accgatccaa gcctcgaggg cgagctcttc ggcgacaaca cccagctcgg ggctgagtac
    13261 gattgctgcg cccaatgccg cgagaccgag aaacaggcag gtcccgagca cgaggcgcgc
    13321 tgtctggaaa accatcgcgg gctccatctg ccccccggct aaagctcgtt gtcgaagtcg
    13381 gcagcaagaa ggcgcgggct ccccgcaggc gcatgctgag ccggagctga cggctggatg
    13441 cacgtcgacg aggtgcaccc catcgttccg ccacattgtt gagggcacct ccccgatcat
    13501 gtcgctggcg ccgattcggc cggcgacgag gtggatcact gcgggctgga ggccatgcag
    13561 gcgcggtgat cggggccggt ttcgggtgga tgaggcgcgg cgacgtcgcg tgctccgcct
    13621 ggtgcggcgg cagcacgccg ccgcgtcagc cgcgacgggg agacatgagc aacctgcttg
    13681 ccttgcgaaa tgggaccgag cttgccggcg agtaccggat cgagcgcgtt cttggcgcgg
    13741 gtggattcgg catcacctac ctcgctgaag agacgtcgct cgcgcggctc gtcaccatca
    13801 aggagtactt cccgtcggat ttcgcggcgc gtgacggcag cgtcgatgcc atgccccgct
    13861 cgctcggctg cgccggcgac tacaaatggg gcctcgaccg cttcatcgcc gaggcgcaga
    13921 cgcttgcccg cttcgagcat ccaaatatcg ttcgcgtcta tcgttacttc cgcgccaaca
    13981 acacaggcta catcgtcctg cgctacgagg acgggcaaag cctcaaggac tggctgaaga
    14041 gcctcggtcg tgcgccgcgc cagcccgagc tcgatcgcat cctcggcccg ctgctcgacg
    14101 cacttgagac gatgcacgcc gccgactacc tgcaccgcga catcgcgccg gacaacatca
    14161 tgatccgcaa ggacggatcg ccggtgctca tcgacttcgg ctcggcgcgc ggcgagatcg
    14221 cgcagcattc gaagacgctg agcgcgctcg tgaagccagg ctacagcccc tacgagcagt
    14281 acgcgacgac cggacgacag caggggccgt ggtcggacat ctactcgctc ggcgccacac
    14341 tctatcaggc ggtgacgggc aagcgccccc ccgatgctcc gacgcgcgtg ctcggcgacg
    14401 agctgaagcc cgcccgggac ggggcgctca gcgcctaccg tccgaaattt ctcgaaggca
    14461 tcgatcgggc gctctctctc gatgtcgagg cgcgtccaca gtcga
//
LOCUS       DAORNH010000088        14455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3164_length_14455_cov_2.792431, whole genome shotgun sequence.
ACCESSION   DAORNH010000088 DAORNH010000000
VERSION     DAORNH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_14455_cov_2.792431"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PK264_10775"
     CDS             <1..402
                     /locus_tag="PK264_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HRD76408.1"
                     /translation="DKWRDGPHTYLGVMTAGFPNLFMITGPQSPGVKSQMIFSCEQHV
                     DFIADCIAHVEGRQARRIEPTRMSEMAWVTHNNEVADRTLYPLANSWYMGANIPGKPR
                     VFMPYVGGVTAYVQKCREVAAKGYEGFEFSA"
     gene            539..907
                     /locus_tag="PK264_10780"
     CDS             539..907
                     /locus_tag="PK264_10780"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD76409.1"
                     /translation="MPSQISVAESPSDAEPVQMIEPMRLAAEEAAKVLRSIGSPYRLM
                     ILCLLVESEKTVSEISDAIGARQSLTSQHLTRLRLDGLVKAERRGHFAYYSLNDTVAK
                     EIVAILYRHYCPKVPGAPGA"
     gene            904..2388
                     /locus_tag="PK264_10785"
     CDS             904..2388
                     /locus_tag="PK264_10785"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HRD76410.1"
                     /translation="MRPPPAVRSWQEPHKLLWAWGLVSGLLVAGAAALAWGSPRFAYE
                     IEVIETPAAAFGVGLALASLVWLAIVPLVRASERQDVTSQRAALTAILVTGLLMRAFA
                     AASTPILEDDWYRYLWEGGLWANGLSPYAIAPADAAEVLDPAVNGLAESAGQILARVN
                     HPHLKTIYPPVAQTAFAIAHLIEPWSFAAWRAVSLLLDLSIALVLLGILDALGRSRLW
                     VALYWLNPVVVKELVNTAHMEGVLMLPVVGALLLALRGRHVAATLVMGLAVGAKVWPL
                     LLLPLILRPLLGDWSRLALALVLLALLGAGIAWPIIVGGLDETSGFLAYAEHWQSNSA
                     LFPALQFIMAQWIGLLGLTEEALASLASRLARAAMGGFALLIALALSLRPIAGPRDLT
                     HRMSMIVFAIVLLSPAQFPWYMVWMIPFLAIMPIPGLLVAVVTTPLYYTYFHYSSRDL
                     GDLFRTRMVWVIWLPVWLVLLADAWRAWRNRAIDSASSEPTPHA"
     assembly_gap    2503..2552
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2553..3121
                     /locus_tag="PK264_10790"
     CDS             <2553..3121
                     /locus_tag="PK264_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD76411.1"
                     /translation="LVVAEPEKGYGAACLAGIAALPPVDIVAFIDGDHSDFPEDLASL
                     VDPIIAGSADFMLASRAVGEREQGSLTPQQVFGNWLATTLIRLFWRARYTDLGPFRAI
                     AKPALERLAMADRNYGWTVEMQIKAAEHGLRHGEVPARYRRRIGVSKVSGTIKGTLMA
                     GYKILSIIGRHALRRWLGRAPQAGRASG"
     gene            3189..3938
                     /locus_tag="PK264_10795"
     CDS             3189..3938
                     /locus_tag="PK264_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRD76412.1"
                     /translation="MADGSGASRVQDFIGDPISEKPPLPRAKLEAIFRDIQENVRFDS
                     ELNGCLNCGICTATCPAAHYYDFSPREIVQLLWTENLEGIYDAMQEKIWSCAQCYTCA
                     ARCPFRNSPGGLVMLMREVAIKHQMQSAKDVLRPFSRVMLKLISTGNQLAPDMINPQH
                     FADWGPNISKVDAPLKLLRKAIPMATLNTTTTAWEVNLKTSVELYTIWEMTGVLDQLE
                     GIDENLFDVIKDIMEEKRDEWDEFLEEQKDD"
     gene            3964..4410
                     /locus_tag="PK264_10800"
     CDS             3964..4410
                     /locus_tag="PK264_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD76413.1"
                     /translation="MGNTVRLHRVIKAPPERVYKAFTDAAAMAKWLSPRGFTCTVHQM
                     EARVGGSFRMSFTNFDSGHTHAFGGSYLELKPFEKLRYTDKFDDPNLPGEIIVTVLLK
                     AVTCGTDLSITQENIPDVIPVEMCYLGWQDSLNQLIDLVETAPPSN"
     gene            4451..4936
                     /locus_tag="PK264_10805"
     CDS             4451..4936
                     /locus_tag="PK264_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD76414.1"
                     /translation="MTTKPSAVPPGYNAVMPYLRVRDASAAMEFYKTAFGARERFRLT
                     MSGKIGHAEVEIAGAVVMLSDAFPDMGLDAPAGSTEAPLPPMMLSVYVDDVDATLARA
                     VQHGAKTVRPAKTEFYGERVAKLVDPFGHVWSLQQRIEDVSPAEMQRRLDAMMASDKK
                     G"
     gene            4940..5218
                     /locus_tag="PK264_10810"
     CDS             4940..5218
                     /locus_tag="PK264_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76415.1"
                     /translation="MRRLPGIVSFILRSAAIGAGLGAGLLALFLATNTGGMRALILES
                     SDPMAPILLLLLGFMTLVGSLYSGAAVMTLPRDERDAIWTHIRKEPSF"
     gene            5233..6567
                     /locus_tag="PK264_10815"
     CDS             5233..6567
                     /locus_tag="PK264_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HRD76416.1"
                     /translation="MTSSNSGDRFTGHGSEWVPAKLTAAEAHTATAWVEQKIDRRSML
                     TNKGRVEDVRDAMWQLEKDGQIVVHRITEANKPIEVKTLYGWTKRIPTDRLWHHKSCG
                     QCGNIPGYPVSLLWLQNQMGIDYLDETDQTSCTAWNYHGSGIGNLESLAAVFLRNFHQ
                     AYVSAKAHGGAIGHYYPLVHCGTSFGNYKEVRHYLLHSAKLRESVTKILGKLGRLVDG
                     KLLIPEEIVHYSEWVHVMRHEIAKRQVVDASKVRATIHPACHVYKMVPEDAIYDDSIL
                     EGNRVAVSTGIIQALGANVIDYKTWYDCCGFGFRHIISEREFTRSFAIDRKIRVAVEE
                     AKSDVMIGHDTGCITTLDKNQWISKAGGEGKGVELPVMADCQFAALVCGADPYKIVQT
                     HWHASPIESPPWRANSATRSKAATRPSTRPCRPSSKSSASTGRKRRSSSRPT"
     assembly_gap    6905..7037
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7205..7279)
                     /locus_tag="PK264_10820"
     tRNA            complement(7205..7279)
                     /locus_tag="PK264_10820"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7245..7247),aa:Val,seq:tac)
     gene            complement(7347..>8330)
                     /locus_tag="PK264_10825"
     CDS             complement(7347..>8330)
                     /locus_tag="PK264_10825"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRD76417.1"
                     /translation="NVLAERRFPANEVIAIASRRSVGIEVSYGEKTLKCQDLATYDFS
                     KVDICLMSAGSGPSKEYSPQIGAKGCIVIDNSSAWRYDQDVPLVVPEVNAAVLEPYMK
                     RNTRKNIIANPNCSTAQMVVALKPLHDAATIKRIVVSTYQSVSGAGKEAMDELWAQTK
                     SKYVPGEEVEPKKFPKQIAFNCIPHIDVFMEDGYTKEEWKMLAETKKILDPKIRVTAT
                     CVRVPVFVGHSEAINVEFEKPITPEYARSILREAPGIMVVDKREAGGYVTPVEAAGEY
                     ATYVSRIRQDATVENGLVLWVVSDNLLKGAALNAVQIAETMINRKLLAKAA"
     assembly_gap    8331..8340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8671..9135
                     /locus_tag="PK264_10830"
     CDS             8671..9135
                     /locus_tag="PK264_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRD76418.1"
                     /translation="MKLLAALTIAALGLSTAATSLRAEAPMTVVESKVPVKAAMDALA
                     KAVEGRGIKVVARIDHAAGAKSAGMELRPTEMLMFGNPKLGTPIIAADPRMGIELPMR
                     VVAWQDAAGKTWIGYTSPDVYKGRYGLKGVDDPLTAMAKALEGLVKGAASGQ"
     gene            complement(9271..9447)
                     /locus_tag="PK264_10835"
     CDS             complement(9271..9447)
                     /locus_tag="PK264_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HRD76419.1"
                     /translation="MSLPAHLAELSEKHRLLERRIEEEMARPSSDLSKVQRLKMEKLK
                     LKDEIARLDKHTRH"
     gene            complement(9915..11621)
                     /locus_tag="PK264_10840"
     CDS             complement(9915..11621)
                     /locus_tag="PK264_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD76420.1"
                     /translation="MFGWFSRRKRPGEAAQVASHPISTGRAGLGPSGHTDTFTRDNLP
                     PDDQWPDLLLERPEFRYPEYLNAAVELTDRMVEKGFGDHIALIGNGRQRTYKELTDWS
                     NRLANALVDNYGVVPGNRVLIRSGNNPAFVAAWLAVTKAGGVAVNTMPMLRAGEIAKI
                     VDKAEVCLALSDSRIADEVVACAKDSRFLKKVVCFDGTSNHDAELDRLALSKPVRFEA
                     VRTGRDDVALLGFTSGTTGVPKATMHFHRDLLIIADGYAREVLGVKPGDIFVGSPPLA
                     FTFGLGGLAVFPLRYGATSALLENASPANLIEIIQTYKATICFTAPTAYRVMLAAMDK
                     GADLSSLRVAVSAGETLPAPVFEDWIRKTGKPMLDGIGSTEMLHIFISNRLGDAKAGS
                     TGRPVTGYEARIVDDDMNDLAPGVPGRLAVRGPTGCRYLNDERQKGYARNGWNLTGDT
                     FVRDADGRFSFAARTDDMIISAGYNIAGPEVEAALLQHPDVAECAVIGVPDVERGQIV
                     EAHIVLKPGLSGDDACAKRLQDHVKAVIAPYKYPRSVRFREGLPKTETGKVQRFRLKQ
                     AV"
     gene            complement(11687..12181)
                     /locus_tag="PK264_10845"
     CDS             complement(11687..12181)
                     /locus_tag="PK264_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD76421.1"
                     /translation="MARVSTDIEAPLSKVRSGAKLELRLWLRMLACVNLVSAELRRRL
                     RSRFDVTLPQFDVLAQLEREPDGLKLVELSRRLMVTKGNLTGLVDTLVEAGLVAREAV
                     PGDRRAQNIRLTRGGTDQFSRMAAAHEAWLAELLGELDRKTLVSLIDELDQVKRSFHL
                     HARR"
     gene            12362..>14455
                     /locus_tag="PK264_10850"
     CDS             12362..>14455
                     /locus_tag="PK264_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HRD76422.1"
                     /translation="MRIVSIGGGPAGLYFALLMKRLHPAHSVTVVERNKPYDTFGWGV
                     VFSDATMDAMRVWDPETAASIEDAFNHWDDIEVWHKGRRMRTTGHGFVGIGRKQLLNI
                     LQARCEALGVELVFERDVESDLEFPDADLIIASDGVNSKIRNRYRGVFRPDVVVRPNR
                     YIWLGTDKLYDAFTFDFRRTEHGWFQAHIYKFDGKTSTFIVETTEDVFTKHGLDTLDQ
                     SQSIAFCEKLFAEVLDGARLMTNARHLRGSAWLNFGRLICGTWSHFNGRSHVVLMGDA
                     AHTAHFAIGSGTKLAIDDAIELANQFERHGHDAGQIPACLAAYEAVRRVDVARIQNAA
                     RNAMEWFEVVGERYADTLEPEQFMYSLLTRSQRSHENLRLRDKAWLEGYERWFGARAG
                     APASTASTSRPLPPMLAPFKLRGTTLANRIVVSPMAMYSAGNGIASDLHLVHLGARAM
                     GGAGLVFAEMTCVAPDARITPGCLGLWSRAHAEALARIVRFVHSSTPAKIGIQLGHAG
                     RKGSTRVGWDGIDQPLEAGNWPLISASALPYQGVNQVPRAMTADDMARVTAEFVEAAR
                     LAVEAGFDVYELHCAHGYLLSSFLSPLTNRRTDAYGGDHAGRARYPLEVFKAIRSVIP
                     ADRPLAVRLSCHDWASGGNTPEDSVIFARIFKDAGADLIDCSSGQVVPDDAPVYGRMY
                     QTPFSDLVRNTVGIPT"
BASE COUNT         2461 a         4740 c         4561 g         2500 t
ORIGIN      
        1 gacaagtggc gggacgggcc gcacacctac ctcggcgtga tgaccgcggg cttccccaat
       61 ttgttcatga tcacgggccc gcagagcccg ggcgtgaaga gccagatgat cttctcctgc
      121 gagcagcacg tcgacttcat cgccgactgc atcgcgcacg tagagggccg ccaggcacgg
      181 cgcatcgagc cgacgcgcat gtccgagatg gcctgggtca cccacaacaa cgaggttgcc
      241 gaccgcacgc tctatccgct cgccaacagc tggtacatgg gtgccaatat cccgggcaag
      301 ccgcgcgtgt tcatgccata cgtcggcggc gtgacggcct atgtgcagaa atgccgggag
      361 gtcgccgcca agggctacga gggcttcgag ttctcagcgt gatcacggtt gcagcgagcc
      421 cttgcacctc ggttcagtcg tgctcatcgc cagacggcac gtccaatgct gcgtttgcgt
      481 aatgagcgcg aattgtctaa catcgaccgt tgcgaccgtc gcggatccgg acccatcgat
      541 gccaagccaa atttccgtcg ctgagagccc atccgacgct gaacccgtcc agatgatcga
      601 gccgatgcgc ctcgcggccg aggaggcagc caaggtgctc cgctcgatcg gcagccccta
      661 ccgcttgatg atcctctgcc tgctcgtcga gagcgagaag acggtctctg agatctcaga
      721 cgcgatcggg gcgcgtcaga gcctcacgtc gcagcacctg acgcggctcc ggctcgacgg
      781 tctcgtcaag gccgagcggc gcgggcactt cgcgtactat tcgctgaacg acaccgtcgc
      841 caaggagatc gtggcgatcc tctaccggca ctactgcccg aaggttcccg gggcgcccgg
      901 cgcgtgaggc cgcctccggc cgtccggtcg tggcaggagc cgcataagct cctctgggcc
      961 tgggggctcg tcagcggcct gctggttgcg ggagcggcgg cacttgcctg gggttccccg
     1021 cgctttgcct acgagatcga ggtgatcgag acgccggccg cggccttcgg cgtcgggctc
     1081 gcgctcgcct cgctcgtgtg gttggcgatc gtgccgctgg tccgcgccag cgagcgccag
     1141 gatgtcacat cgcagcgggc cgcgctgacg gcgatcctcg ttaccgggtt gctgatgcgc
     1201 gccttcgcgg cagcaagcac gccgatcctc gaggacgact ggtatcgcta tctctgggag
     1261 ggcgggctgt gggccaacgg cctgagcccc tatgccatcg cccctgccga tgccgccgag
     1321 gtgctcgatc cggccgtcaa tgggctcgcc gagagcgccg ggcagatact ggcccgcgtc
     1381 aatcatccgc acctcaagac gatctacccg ccggtggcgc agacggcgtt cgcaatcgcg
     1441 catctgatcg agccgtggag ctttgccgca tggcgcgcgg tgtcgctgct gctcgacctc
     1501 agcattgcgc tcgtgctgct cggcattctc gatgcgctcg gccggtcgcg actctgggtg
     1561 gcgctctact ggctcaaccc ggtcgtcgtg aaggagcttg tcaacaccgc ccatatggag
     1621 ggcgtgctga tgttgcccgt ggtcggcgcc ctgctcctcg cgctgcgcgg ccgtcatgtc
     1681 gccgcgaccc tggtgatggg tctcgccgtc ggtgccaaag tctggccgct gctgctgctg
     1741 ccgctgatcc tgcgtcccct cctcggcgac tggtcgcggc tcgccctcgc cctcgtcctg
     1801 ctcgccctgc tcggcgccgg tatcgcttgg ccgatcatcg tcggcgggct cgacgagacc
     1861 tcggggttcc tcgcctatgc cgagcactgg caatcgaaca gtgcgctgtt cccggccctc
     1921 cagttcatta tggcccaatg gattggcctc ctcggcctga ccgaagaagc gctcgcaagc
     1981 ctcgcctcgc gcctcgctcg tgccgcgatg ggcggcttcg cattgctgat cgcgctcgcc
     2041 ctgtcgctcc ggccgattgc cggaccgcgc gatctgacgc accggatgag catgatcgtg
     2101 ttcgccatcg tcctgctgag ccctgcgcag tttccctggt acatggtatg gatgatcccc
     2161 ttcctggcca tcatgccgat cccggggctc ctcgttgccg tggtgacgac gccgctctac
     2221 tacacctact tccactactc ctcgcgcgat ctcggcgatc tcttccgaac acggatggtc
     2281 tgggtgatct ggctgccggt ctggctggtg ctcttggccg atgcctggcg cgcgtggaga
     2341 aacagggcca tcgacagcgc cagcagcgag ccgacacctc atgcgtaacg gacagcgaat
     2401 tggcgtcgtc atcccggcgc tcaacgagga gggtgcaatc gggctcgtcg tcgccgagat
     2461 cccatcctgg gtcgacgcca tcgtggtcgc cgacaacggc tcnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctagtc gtcgccgagc ctgagaaagg
     2581 ctacggcgcc gcctgcctcg ccggcatcgc agccttgccc cccgtcgaca tcgtcgcctt
     2641 catcgacggc gatcacagcg acttccccga ggacctcgcg agcctcgtcg atccgatcat
     2701 cgcgggctcc gccgacttca tgctcgcctc ccgcgccgtg ggcgagcgtg agcagggctc
     2761 gctcacgccg cagcaagtgt tcgggaactg gcttgccacg acgctgatcc gcctcttctg
     2821 gcgcgcgcgc tataccgacc tcgggccatt ccgggcgatc gccaagccgg cgctggaaag
     2881 gctcgccatg gccgatcgca actacggctg gaccgtcgaa atgcagatca aggccgcaga
     2941 gcacggactc cgccacggcg aggtgcccgc ccgctaccgg cgccgcatcg gcgtctccaa
     3001 ggtctcgggc acgatcaagg gcaccctgat ggccggctac aagatcctct ccatcatcgg
     3061 ccggcacgcg ctgcgccgct ggctcgggcg agcaccgcaa gcagggcgcg catccggata
     3121 agcgcttgcc gggaggaccg acagacgttt atattcgctc ttgcgaatat tcgttgctgg
     3181 agctggccat ggccgacgga tcaggagcat cgcgcgtgca ggacttcatc ggggatccga
     3241 tctccgagaa gccgccactt ccgcgcgcca aactcgaggc gatcttccgc gacatccagg
     3301 agaacgtgcg cttcgacagc gagctcaacg gctgcctcaa ctgcggcatc tgcacggcga
     3361 cctgcccagc ggcgcattac tacgacttct cgccgcgcga gatcgtgcag ctactatgga
     3421 ccgagaatct cgagggcatc tacgatgcca tgcaggagaa gatctggtcc tgcgcccagt
     3481 gctacacgtg cgccgcgcgc tgcccgttcc gcaactcgcc gggcgggctc gtgatgctga
     3541 tgcgcgaggt ggcgatcaag caccagatgc agtccgcaaa ggacgtgctg cgacccttca
     3601 gccgtgtcat gctgaagctc atctcgacgg gcaaccagct cgcgcccgac atgatcaatc
     3661 cccagcattt cgccgactgg ggccccaaca tctcgaaggt cgatgcgccg ctgaagctcc
     3721 tgcgcaaggc gatcccgatg gcgacgctca acacgacgac caccgcttgg gaggtgaacc
     3781 tcaagacctc ggtcgagctc tacacgatct gggagatgac cggcgtcctc gaccagctcg
     3841 aggggatcga tgaaaacctc ttcgacgtca tcaaggacat catggaggag aagcgcgacg
     3901 agtgggacga gttcctggag gagcagaagg acgactgagc cagtcaagcg aggagaaccg
     3961 cccatgggta acactgtcag gctgcatcgc gtgatcaagg cgccgccgga gcgcgtctac
     4021 aaagcgttca cggacgcggc cgccatggcc aagtggctct cgccgcgcgg attcacctgc
     4081 accgtgcatc agatggaggc gagggtcggg ggcagcttcc gcatgtcctt caccaacttc
     4141 gacagcggcc acacacacgc attcgggggc tcgtatctcg agctgaagcc cttcgagaaa
     4201 ctccgttaca cggacaagtt cgatgatccg aacctgccgg gcgagatcat cgtcaccgtc
     4261 ctgctgaagg ccgtgacttg cggcactgac ctctcgatca cgcaggagaa catcccggac
     4321 gtgatcccgg tcgagatgtg ctacctcggc tggcaggatt ccttgaacca gctgatcgat
     4381 ctcgtcgaaa ccgcgccacc gtcgaactga tgctccgacc gagccgattc caggcccgag
     4441 gagggcgatc atgaccacga agccgtctgc ggtaccgccc ggctacaatg ccgtgatgcc
     4501 atacctgcgc gtgcgtgatg cgtccgcagc gatggagttc tacaagacgg ccttcggcgc
     4561 ccgcgagcgt ttccggctca ccatgagcgg aaagatcggc cacgccgaag tcgagattgc
     4621 gggcgcagtc gtcatgctgt cggatgcgtt ccccgatatg gggctcgatg ctcccgccgg
     4681 ctccaccgag gcgccgctgc cgcccatgat gctgtccgtg tacgtcgacg acgtcgacgc
     4741 gacgctcgca cgcgcagttc agcacggcgc caagaccgtg cggcccgcca agaccgagtt
     4801 ctacggcgag cgggtcgcaa agctcgtcga tcccttcggc catgtctggt cgctgcagca
     4861 gcgcatcgag gacgtatcgc ccgccgagat gcagcgccgg ctcgacgcca tgatggcgtc
     4921 ggacaagaag ggctgaggca tgcgcaggct accgggcatc gtcagcttca ttctccgctc
     4981 cgccgcgatc ggcgccggct tgggcgccgg gctattggca cttttccttg ccaccaacac
     5041 gggcggcatg cgtgcgctga tcctcgagag ctccgacccg atggcgccga tcctcctcct
     5101 gctgctcggc ttcatgacgc tcgtcggcag cctctactcg ggtgccgccg tcatgacgct
     5161 gccgcgcgac gagcgcgatg ccatctggac tcatatccgc aaagaaccaa gcttctaggg
     5221 agagacgacc ccatgaccag ctccaactca ggcgatcgct tcaccggcca cggttccgag
     5281 tgggttccgg ccaagctcac cgcggcggaa gcccacactg cgacggcctg ggtcgagcag
     5341 aagatcgacc ggcgctcaat gctcaccaac aagggccgcg tcgaggacgt gcgcgatgcc
     5401 atgtggcagc tcgagaagga cggccagatc gtcgtccatc gcatcaccga ggccaacaag
     5461 ccgatcgagg tgaagaccct ttacggctgg acgaagcgca ttccgaccga ccgcctctgg
     5521 catcacaaga gctgcggcca gtgcggcaac atcccgggct atccggtctc cctcctctgg
     5581 ctgcagaacc agatgggcat cgactatctc gacgagaccg accagacgag ctgcaccgca
     5641 tggaactacc acggctcggg catcggcaat ctcgagagcc ttgccgcggt gttcctgcgg
     5701 aacttccacc aggcatacgt ttcggcgaag gcgcatggcg gagccatcgg ccactactat
     5761 ccgctcgtcc actgcggcac ctcgttcggc aactacaagg aggtgcgcca ctacctgctc
     5821 cactccgcga agctccgcga gagcgtcacg aagatcctcg gcaagctcgg ccgcctcgtc
     5881 gacggcaagc tgctcatccc cgaggagatc gtgcactact ccgagtgggt gcacgtgatg
     5941 cgccacgaga tcgcgaagcg ccaggtggtc gacgcctcca aggtgcgcgc caccatccat
     6001 cccgcctgcc acgtctacaa gatggtgccc gaggacgcga tctacgacga cagcatcctc
     6061 gaggggaacc gcgtcgccgt ctcgaccggc atcattcagg cgctcggcgc caacgtgatc
     6121 gactacaaga cctggtacga ctgctgcggc ttcggcttcc gccacatcat ctcggaacgc
     6181 gagttcacgc gctcgttcgc gatcgaccgg aagatccgcg tcgcggtcga ggaggcgaag
     6241 tcggatgtca tgatcggcca cgacacaggc tgcatcacca ccctcgacaa gaaccagtgg
     6301 atctccaagg cgggcggcga aggcaagggc gtcgaactcc ccgtgatggc cgactgccag
     6361 ttcgcagcgc tcgtctgcgg tgccgacccc tacaagatcg tccagaccca ctggcacgcc
     6421 tcgccgatcg agtcgccgcc ctggcgcgcg aactcggcca cacggtcgaa ggcagcgacc
     6481 aggccatcta cccgcccatg tcgacccagc tcgaaaagct cggcatcgac tggcagaaaa
     6541 agaaggtcga gttcgaggcc tacctgaaga ccatccaaac cggcgccgcc gaccagcttt
     6601 acgacccgcg cctgcgcctt acgagcggcc ccggtttcaa gcctgtgaag cgcgaagtaa
     6661 tccctccgcc gcctccagca gagtgagcca acgcagcggc ggcaatgctt aatgcaacgc
     6721 ggccgccctc acgcgtcgat gacgacatac tcgcccgcga cctcgacggg gaaagtctcc
     6781 gccttgtagg gaccttcgac gagcgcggcg ccgggcgcga tctcgacggg gaagctcatc
     6841 aggcggagcc gcttcgggtc gcagtaggag cggcccgtgc ggacatcgaa ctcccagccg
     6901 tgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnagg gcgaacagct cgcccgcaac gttcaagacc aggatggcct
     7081 tgccttcgac cgagaccagt ttgcgcgtgc ctggcggcag ctcatcggca cgggcgacga
     7141 catggcgcgg catggttcac ctcgggatcg atgctatggc catcgtagcg atggtgggca
     7201 gcgatggtgg gcggtgagag ggtcgaactc ccgacatctt cggtgtaaac gaagcgctct
     7261 gccactgagc taaccgccct gcacctgtgc aaacgaggtc tggctcgacc caactcagga
     7321 acgagccgac tgtgatagcg ctcccatcaa gccgctttgg cgagcagttt tcggttgatc
     7381 atggtctcgg cgatctggac ggcgttgagg gcggcgcctt tcaggaggtt gtccgagacg
     7441 acccagagga cgaggccgtt ctcaacggtc gcgtcctggc ggatgcgcga gacgtaggtc
     7501 gcgtactcgc cggccgcctc gacaggcgtc acgtagccgc cggcctcgcg cttgtcgacg
     7561 accatgatgc ccggtgcctc gcgcaggatc gagcgggcgt actcgggcgt gatcggcttc
     7621 tcgaactcga cgttgattgc ctcggaatgg ccgacgaaga ccggaactcg cacgcacgtc
     7681 gcggtgaccc tgatcttggg atcgaggatc ttcttcgtct cggcgagcat cttccattcc
     7741 tccttcgtgt agccgtcctc catgaagacg tcgatgtgag gaatgcagtt gaaggcgatc
     7801 tgcttcggga acttcttggg ctcgacctcc tcgcctggca catacttgct cttggtttgc
     7861 gcccagagct cgtccatggc ctccttgccg gcgccggaaa cggactggta ggtcgacacg
     7921 acgatccgct tgatggtggc ggcatcgtgc agcggcttca gggcgacgac catctgcgcc
     7981 gtcgagcagt tcgggttggc gatgatgttc ttgcgcgtat tgcgcttcat gtagggctcg
     8041 agcaccgcgg cgttgacctc tgggacaacg aggggcacgt cctgatcata gcgccaggcc
     8101 gacgagttat cgatgacgat gcagcccttg gcgccgatct gcgggctgta ttccttcgag
     8161 gggcccgagc cggcgctcat gaggcagatg tcgaccttcg agaaatcgta ggtcgcgagg
     8221 tcctggcatt tgagcgtctt ctcgccgtag ctgacctcga tcccgaccga ccggcgggag
     8281 gcgatggcga tgacctcgtt ggcgggaaag cgccgctccg cgaggacgtt nnnnnnnnnn
     8341 cagcatctca cggcccacat tgcccgtggc gccgacgaca gcgaccctgt agcccatggc
     8401 gttaatccag gtcctctcga ggaagttcca caatggatgg agcagcgccg atcgccgccc
     8461 gctcacgtcc tcgatagccg attagtctca atgctccgtg ccttcaagca gcaatttggc
     8521 cgtgcgcacg aattgcgcgg cgccgtggcg ccacccgatc acggaggatc acggaggatc
     8581 aaaagcaagt gccaggaaac gaatgggcgg gcggcgcggg cgaactgccg ttacggtcgc
     8641 aggcccacac gtttcgcaag gagaccgcac atgaagctct tggccgcgct gacaattgcc
     8701 gcccttggcc tgtcgacggc cgccacaagc ctcagggccg aggcgccgat gacggtcgtc
     8761 gagagcaagg tgccggtcaa ggccgccatg gacgcgcttg ccaaggcggt cgagggccgt
     8821 ggcatcaagg tcgtggcgcg gatcgaccac gctgcaggcg ccaagtccgc cggcatggag
     8881 ctgcggccaa cggaaatgct catgttcggc aacccgaagc tcggcacgcc gatcatcgcg
     8941 gccgaccctc ggatgggaat cgagttgccg atgcgcgtcg tcgcctggca ggacgccgcc
     9001 gggaaaacgt ggatcggcta tacctcgccg gacgtctaca agggccgata cggcctcaag
     9061 ggcgtcgatg acccgctgac tgcgatggcc aaggccctcg aagggctcgt gaaaggtgcc
     9121 gcaagcggtc aatgatccaa gcgctgcgca gacggcactg aactcgcacc ttgaaatagc
     9181 aagccccgcg gtcggttacg gccggcgggg catcattacg tgctattact ggctcggccg
     9241 ttgtccggcc tgtcgacaat cccggctcga ctagtgccgg gtgtgcttgt cgagacgagc
     9301 gatctcgtcc ttcagcttca gtttttccat cttcaggcgt tgcactttgg agaggtcgga
     9361 ggacggtcgg gccatctcct cctcgatgcg ccgttcgagc aggcggtgct tctcgctcaa
     9421 ctctgcaagg tgtgccggca gggacatccg gtgctccgta tcgcagttga cgatgacgtg
     9481 agtgtgacag agccgcgtgc cgatgtccat cggatgaggc cgcgcctgtg gtgatttttc
     9541 cgcgcgcgca ccgatctcgc agcgcggtcc cggaattagg cttgatgcgc gatgaacaca
     9601 atggcgccaa tacagcccgg atgtttcggc aagtgccgac cattcacact cctcgagagg
     9661 atgattcatg ggtagcagcg gggattgctg acggcggggt ttggggcggc ggcacatcct
     9721 ggggttgccc gccggcctcg atactgcggc gcatgagcaa catgctccaa tcgacgacaa
     9781 tatcgttcga ttggtcgacc caggagcgtt cgacgaccaa ctggccgaag gcatgcggag
     9841 cagggcgcgt gccctgaccg ggctggcggc cgaggcgatc cgcgccgtgc gctgcatcga
     9901 gcaggttcgc cctgctacac ggcctgcttg agcctgaagc gctgcacctt gccggtctcg
     9961 gttttcggca ggccttcgcg gaaccggacc gatctcggat acttgtaggg cgcgatcacg
    10021 gccttcacgt gatcctgcaa tctcttggcg caggcatcgt caccgctgag accgggtttc
    10081 aagacgatgt gcgcttcgac gatctggccg cgctcgacat ccggcacacc gatgacggcg
    10141 cactcggcga catccggatg ctggagcagt gcagcctcca cctcgggacc cgcgatgtta
    10201 tagccggccg agatgatcat gtcgtcggtc cgcgccgcga acgagaaccg gccgtcggcg
    10261 tcacggacga aggtgtcgcc ggtcaggttc cagccattgc gcgcatagcc cttctgacgc
    10321 tcgtcgttca ggtagcggca gccggtcgga ccacgcacgg ctaggcgtcc aggcacgccc
    10381 ggggccaggt cgttcatgtc gtcgtcgacg atgcgggcct cgtagcccgt caccggccgt
    10441 ccggtggagc cggctttcgc gtcgccgaga cggttggaaa tgaaaatgtg cagcatctcg
    10501 gtcgagccga ttccgtcgag catcggcttc cccgtcttcc tgatccaatc ctcgaacacg
    10561 ggcgcaggca gcgtctcgcc ggctgagacc gcaacgcgca gcgacgaaag gtcggcgccc
    10621 ttgtccatcg cggcgagcat gacgcgatag gcggtgggcg ccgtgaagca gatcgtcgcc
    10681 ttgtacgtct ggatgatctc gatgaggttt gccggagacg cgttctcgag cagcgccgac
    10741 gttgcgccgt agcgcagcgg gaacacggcg agccctccga ggccgaacgt gaaggcgagc
    10801 ggaggcgagc cgacgaaaat gtctccgggt ttcacgccga gcacttcgcg cgcatagccg
    10861 tcggcgatga tcaggagatc gcgatggaag tgcattgtcg ctttcggcac gccggtggtg
    10921 ccggacgtga agccgagcag ggcgacgtca tcgcggccgg tcctcacggc ctcgaacctt
    10981 accggcttgc tgagcgcgag ccgatcgagc tcggcgtcgt ggttcgaagt gccgtcgaaa
    11041 catacgacct tcttcaggaa acggctgtcc ttggcgcagg cgacgacctc gtcggcgatc
    11101 cggctgtcgc tgagcgcgag gcagacctcg gccttgtcga cgatcttggc gatctcgcct
    11161 gcccggagca tcggcatagt gttgacggcg acaccaccgg ccttggtcac ggcgagccag
    11221 gcggcgacga aggccgggtt gttgcccgat ctgatcagca cgcgattgcc ggggacgacg
    11281 ccgtagttgt cgacgagcgc gttggcgagg cgatttgacc agtcggtcag ctccttgtag
    11341 gtgcgctggc ggccgttgcc gatgagcgcg atgtggtcgc cgaagccctt ctcgaccatg
    11401 cgatcggtga gctcgacggc tgcattcaga tactccggat agcggaactc cggccgctcg
    11461 aggaggaggt ccggccattg gtcgtcgggc gggagattgt cgcgcgtgaa cgtatcggtg
    11521 tgtccgctag gcccgaggcc ggcgcggccg gtgcttatcg gatgcgaggc aacctgagcg
    11581 gcctcgccgg ggcgtttgcg tcgcgagaac catccgaaca ttcctggcct cctgttcgct
    11641 gcgaccttgc ctcagcgcct atggtccgac cgcacgggcc tctcactcag cggcgagcgt
    11701 gcagatggaa cgagcgcttg acctgatcga gttcatcgat cagtgaaacg agtgtcttcc
    11761 tgtcgagctc gccgagaagc tcagccagcc aagcctcgtg agccgcggcc atcctcgaga
    11821 actggtctgt gccgccacgg gtcagtctga tgttctgtgc acgccggtcg ccgggtacag
    11881 cttccctggc gacgaggccc gcctcgacga gtgtgtcgac gaggccggtc agattgcctt
    11941 tcgtgaccat gagccggcgc gacaattcga cgagcttcag gccgtccggc tcgcgctcga
    12001 gctgagccag cacgtcgaac tgcggcaacg tcacgtcgaa ccgggatctc aggcgtcgcc
    12061 tcaactcggc ggagacgagg ttgacacacg cgagcatgcg cagccagagg cgcagctcga
    12121 gcttggcgcc ggatcgcacc ttgctgagcg gcgcctcgat gtctgtcgat acgcgcgcca
    12181 tcttcgtcac ctccgcgggc tcggcacctc ggccggcccg gccagcccgg cacttccgcg
    12241 ggccggcacg ttcgtgggcc agcccgacat cgatcaggaa agtttagatt tgaacaatct
    12301 aggtttcaac tataaagttg ccgtcctgag cagcgcctgc ctccagcctg gggagcgtgt
    12361 catgcggatc gtgtcaatcg ggggcgggcc ggccggcctc tacttcgcgc ttctgatgaa
    12421 gcgcctgcat ccggcgcact cggtcaccgt cgtcgaacgc aacaagccct acgacacgtt
    12481 cggctggggc gttgtgttct cggacgcgac catggacgcg atgcgagtct gggatccgga
    12541 gactgccgcg agcatcgagg acgcgttcaa tcactgggac gacatcgagg tctggcacaa
    12601 gggccgccgc atgcgtacga cgggccacgg cttcgtcggc atcggccgca agcaactgct
    12661 gaacatcctg caggcgcgct gcgaggcgct cggcgtcgag ctcgtcttcg aaagggatgt
    12721 cgagagcgat ctcgagtttc cggatgccga cctgatcatc gcctccgacg gggtcaattc
    12781 gaagatccgc aaccgctacc gtggtgtgtt ccggcccgac gtggtggtcc gccccaaccg
    12841 ctacatctgg ctcggcaccg acaagctcta tgacgccttc accttcgact tccgccggac
    12901 cgagcacggc tggttccagg cccacatcta caagttcgac ggcaagacct cgaccttcat
    12961 cgtcgagacg accgaagatg tgttcacgaa gcacggcctc gacacgctag accagagcca
    13021 gtcgatcgcg ttctgcgaaa agctgttcgc cgaggttctc gacggcgcgc gcctcatgac
    13081 caatgcgcgg catctgcggg gctcggcgtg gctcaacttc ggccgcctga tctgcgggac
    13141 ctggtcgcac ttcaatggca gatcgcatgt cgtgctgatg ggggacgccg cccacaccgc
    13201 ccatttcgcg atcggctccg gcaccaagct tgccatcgac gacgccatcg agctcgccaa
    13261 ccagttcgag cgccacggcc acgacgccgg gcagatcccg gcgtgccttg cggcgtacga
    13321 ggccgtgcgc cgggttgacg tggcgcgcat ccagaacgcg gcgcgcaatg ccatggagtg
    13381 gttcgaggtc gtgggcgagc gctatgccga cacgctcgag ccggagcagt tcatgtactc
    13441 gctgctgacc cgctcgcagc gcagccacga gaacctgcgc ctgcgcgaca aggcctggct
    13501 cgagggctac gagcgctggt tcggcgcgcg cgccggcgcg cccgcctcga cagcgagcac
    13561 aagccgcccc ctgccgccga tgctcgcgcc gttcaagttg cgcggcacga cgctcgccaa
    13621 ccggatcgtc gtctcgccga tggcgatgta ttcggccgga aacgggatcg caagcgacct
    13681 ccatcttgta caccttggcg cacgcgccat gggcggagcg gggctcgtgt tcgcggaaat
    13741 gacatgcgtc gccccggatg cccgcatcac gcccggctgc ctcgggctct ggagccgggc
    13801 gcacgccgag gcactcgcgc ggatcgtgcg tttcgtccat tcgagcacgc cggcgaagat
    13861 cggcatccag ctcggtcatg caggccgcaa ggggtcgacg cgggtcggct gggacggcat
    13921 cgaccagccg ctcgaggcag gcaactggcc gctgatctcg gcatcggcac tgccctacca
    13981 aggcgtaaac caggtgccgc gtgccatgac tgcggacgac atggcgcgcg ttacggcgga
    14041 attcgtggag gcagcgcgtc ttgccgtcga ggcgggcttc gacgtctatg aactgcactg
    14101 cgcccacggc tacctgctct cgagcttcct gtcgccgctc accaatcggc gcaccgacgc
    14161 ctatggcggg gatcacgccg gccgcgcgcg ctatccgctc gaggtgttca aggcgatccg
    14221 ttccgtcatc cccgccgacc ggccgctcgc ggtgcgcctc tcctgtcacg attgggcaag
    14281 cggcggaaac actccggagg attccgttat cttcgcccgc attttcaagg atgccggtgc
    14341 cgatctcatc gattgctcat cgggacaggt ggtaccagac gacgcacccg tctacggacg
    14401 catgtaccag acgccgttct cggacctcgt ccgtaacacg gtcggcattc cgacc
//
LOCUS       DAORNH010000089        14436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3167_length_14436_cov_2.481747, whole genome shotgun sequence.
ACCESSION   DAORNH010000089 DAORNH010000000
VERSION     DAORNH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3167_length_14436_cov_2.481747"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1213
                     /gene="cysK"
                     /locus_tag="PK264_10855"
     CDS             230..1213
                     /gene="cysK"
                     /locus_tag="PK264_10855"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRD76423.1"
                     /translation="MTDHNRQPAVKLDAPGRGRIYNSIVETIGNTPLVRVPGLTAEKG
                     LKADLCLKLEFFNPIASVKDRIGVNMILELEAAGRIKPGATLIEPTSGNTGIGLAFVA
                     ASRGYKLILTMPDSMSIERRKMLAYLGARLELTPREKGIKGSIARAEELLAEIPGSVI
                     PSQFSNPANPDIHKRTTAEEIWRDTGGEVDAIISGVGTGGTITGLGQVLKPRRAGLKI
                     IAVEPELSPVLSGGTHSPHPIQGIGAGFVPEILDRSVIDEVLTVSGKDAIDTARLLAA
                     LDGIPVGISSGAAVAVALRVAGRPEMAGKRIVTIIPSFAERYLSTVLFEGI"
     gene            complement(1232..2758)
                     /locus_tag="PK264_10860"
     CDS             complement(1232..2758)
                     /locus_tag="PK264_10860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRD76424.1"
                     /translation="MTEVQRRARAAAPAPTETAADGLPFRVAPHNIEAEQALLGAIFI
                     NNESHDRVSSFLTAEHFFDPLHARIFETAAKLIAAGKQATPITLRTFFENEEPIEPGL
                     TVPLYLGKLAASATTIINARDYGQTIYDLAIRRQLITIGEEMVNQAFDTPVDHPPKVQ
                     IEEAESRLFSLAETGKFGQGFLGFREALTGAIEMANNAFKRAGGLSGISTGLADLDNK
                     MGGLQSSDLIVLAGRPSMGKTALATNIAFNIAKAFKTEKQTDGTEKVVDGAVVGFFSL
                     EMSAEQLATRILSEQSEISSEKIRRGMIDEADFGRLVRASQEMSSVPLYIDQTGGITI
                     AQLAARARKLKRQRGLGLLIIDYLQLLSGSGKRSSDSRVQEVSEITTGLKALAKELNV
                     PIIALSQLSRQVETREDKRPQLSDLRESGSIEQDADVVMFVFREEYYVERTKPAEGTP
                     EFLTWQANMDRVHGKAEVIIGKQRHGPIGTVTLAFEGNFTRFSNLAYDAHSQSLPPQY
                     "
     gene            3021..4205
                     /locus_tag="PK264_10865"
     CDS             3021..4205
                     /locus_tag="PK264_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HRD76425.1"
                     /translation="MERFSNAVTYLGSHRPERPVFGMRPEAAGRAARWFLQNFPGDVA
                     YAYKANSHPLIIGSLYGAGIRHFDVASLPELEDAATIPGAILHFMHPVKSRNAIRRAY
                     SLGVRSFSLDSEEELDKIVEETNGARDLALSVRLAVPAKNSRLPLERKFGIAMSKAAP
                     LLLRTRQAAAELGLTFHVGSQTVTPDAYTLALAEIGKLIKRAGVVVDKIDVGGGFPSR
                     YSEEAPTPLQHFVDAIKLGFERLPVKETCKLMCEPGRALVAEAESLIVRIDARRGNEL
                     FINDGGYGALFDAAHLGFAFPVRLVTGGPRDAADLMPFELWGPTCDSVDRMKGPFMLP
                     DNVREGDYLELGNLGAYGRAIAGKFNGYGQYDEVLLGDEPMYSMYRGSNATAVPDPEG
                     VR"
     gene            complement(4219..4965)
                     /locus_tag="PK264_10870"
     CDS             complement(4219..4965)
                     /locus_tag="PK264_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRD76426.1"
                     /translation="MSNLMTLAQDPAAWAALATLIAMEVVLGIDNLIFIAILTNKLPE
                     QYRAKARRIGISLALIMRLVLLGTIAWIVQLTAPLFTLFGHGISWRDIILIAGGLFLV
                     WKATKEIHHRVDPHPNQDVFDTGQASLTFASAITQILLLDLVFSIDSIITAVGMTEHV
                     PIMVIAVLVAVAVMMLAATPLANFVNANPTIVMLALGFLLMIGAVLIAEGFGAKIPKG
                     YIYAAMLFSAFVEGLNMLSRRRRANPRTKS"
     gene            5071..5289
                     /locus_tag="PK264_10875"
     CDS             5071..5289
                     /locus_tag="PK264_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76427.1"
                     /translation="MIRIEHVEPLNASRSKFRPSGIGFYVLPEQVTLIRPTRDPNVSE
                     VRLQGGAVYFAGASPAALEQALLDNARR"
     gene            5546..6706
                     /locus_tag="PK264_10880"
     CDS             5546..6706
                     /locus_tag="PK264_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76428.1"
                     /translation="MRAINAVGLVACSLATTLPMLVSARVLQGVGAALTFACGPAIAT
                     ALGGEGRRAEMLSFHAMVIAIGSAAGPLVGGMLVDLWGWPAVFWARAPLPLAALLLSS
                     AMSPLSQAPPRGGMDWLNGMLVVLAMAGAGSTLGFAAGTATPLVALLAAVAIVAWWVQ
                     GQRSAAPVLDPAHFRQTEFTVLNLSTIAINLVAFAVLLIAPYYLVRMVGLSNRDAGLV
                     LSLPFFGMLAGAWLVPRLTTSLNAGRLAFAGLCSVAVMQGAIGALEATSPMALIMAAF
                     GLHGMGLGVFQVAAATIVTGTLPLEQRGVAGSLGIVMRTIGVVAGAFVLATLFDTMSG
                     TDAVGERERIEAGVAAFKATFIYSSLALAIFILATLAWPRTWHWELDDAARR"
     gene            6772..>7704
                     /locus_tag="PK264_10885"
     CDS             6772..>7704
                     /locus_tag="PK264_10885"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HRD76429.1"
                     /translation="MNSPVCASGPNMVDRTVLIMRKCRSAALGRRLAGSAATLALVLG
                     SAAVAQQPHAGLNGPPPAASGPGPSGPSSGATVTYQVRSKNGHFYVPLTIASRAVTML
                     VDTGATTVVLSAADAERLGIDVRRLRYDTATATAAGRLMVASIELHDVRIGRIYVGRV
                     AATVLPENSGKQSLLGMSVIQKLGRVTVEGESLTIVGVIIPGALVMFSVAGVPPASNA
                     RTPPLRPAPPPTSVSGARLGWGALALSADAYGWSRGYATRSEAERVALDNCRKHAKRD
                     QCDVRAFDGSLCAAVATWSFMEGSERRFGNIGNFG"
     assembly_gap    7705..7803
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7810..8238
                     /gene="mutT"
                     /locus_tag="PK264_10890"
     CDS             7810..8238
                     /gene="mutT"
                     /locus_tag="PK264_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HRD76430.1"
                     /translation="MPSAVCITPRRLVLVAAVALIDVDNRVLIAQRPPGKAMAGLWEF
                     PGGKVDPGETPEEALVREITEELGIEVCEPCLAPLTFASHAYPDFHLLMPLFICRNWD
                     GEISAREGQQLKWVRAMRLSDYPMPPADLPLLPALRDLLG"
     gene            complement(8262..8897)
                     /locus_tag="PK264_10895"
     CDS             complement(8262..8897)
                     /locus_tag="PK264_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRD76431.1"
                     /translation="MGLEDLWLFILAGLLLNITPGPDIALILSRSTQHGTRVGMGAAL
                     GVGAGAFAHIAAAAIGLSAVLVTSATAFTIVKWVGALYLVYLGVQMVRATFKGTAGSA
                     GLEREEPVTFWQAFLQGALTNALNPKVAIFFLAFLPQFVDPATPSKVLAFVTLGLLFN
                     AVGTAWNLAVAWFAGQLAESRAYVRIKVWLERAIGGLFIVVGVRLALSERP"
     gene            9111..10046
                     /locus_tag="PK264_10900"
     CDS             9111..10046
                     /locus_tag="PK264_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD76432.1"
                     /translation="MSAKASTTIAASDWGLLAILSVLWGGSFFFAGVAVRELPPLSIV
                     LARVAIAALLLIPLHWLLIGALPRARSAWLAFLVMAVLNNVIPFSLIVTGQKEIASGL
                     ASVLNATTPLFAVIVMAAFGDEKLIARKVAGVIVGLAGVAILRGTTPMSLGDGATLGV
                     ALCLAGALSYGFAGLWGRRHLAGVPPLTSATCQLICSTAVMAALASLVDAPWRLAVPS
                     VATLWSLLGLAALSTALAYVVFFQILVRAGATNVMLVTLLIPLTSILLGHLVLDEAIR
                     PREIVGALVIASSLLIVDGRIFSALRRDRDTSAAP"
     gene            complement(10059..11282)
                     /locus_tag="PK264_10905"
     CDS             complement(10059..11282)
                     /locus_tag="PK264_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD76433.1"
                     /translation="MLAIDFADPATNADPFAIFARLRAEDPAHWSEAMKAWVVTRYDD
                     VKAISLNNAEVSANRLAPFFAAAPEARRSTYANLMTYLGGWMVFRDPPDHTRLRRLFT
                     KAFTSRSLDSLKPRISDLVDMLFDDMAAKARAGDTADWIADFAYALPATVIMDLLGVP
                     RGDLKRVKLWSDDIALFIGTARATPDKYDRAERGARAMADYFRGIVADRTAGPRDDII
                     SQMVIARDEKEALTSDEVIATSILLLFAGHETTTNLLGNGLYHAMRFRGQWERLVADT
                     GLIEPAIEEWLRYDGPSSALARVVVDDIEFGGRLLKKGQRVFAFTNAANRDGAQFPDP
                     DRFDIARTPNAHLTFGHGIHFCLGAPLARLEAQIAVRRLAERFPRVRLAGSGAPEWSD
                     SLILRGIKSLPVVLG"
     gene            complement(11327..12676)
                     /gene="argG"
                     /locus_tag="PK264_10910"
     CDS             complement(11327..12676)
                     /gene="argG"
                     /locus_tag="PK264_10910"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRD76434.1"
                     /translation="MSTILQSLPKGQKVGIAFSGGLDTSAALLWMKLKGARPFAYTAN
                     LGQPDETDYEAIPRKAMDYGAEKARLVDCRAQLVHEGIAAIQAGAFHISTGGITYFNT
                     TPLGRAVTGTMLVTAMREDDVNIWGDGSTFKGNDIERFYRYGLITNPSLRIYKPWLDQ
                     AFIDELGGRKEMSEFMTKHGFGYKMSAEKAYSTDSNLLGATHEAKDLEELSSSIRIVN
                     PIMGVAFWREDCDVKPETVKVRFHEGLPVALNGQEFSDPVVLMTEANAIGGRHGLGMC
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRISGMKLGRLLYQG
                     RWFDSQALMLRETAQRWVARAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSMERV
                     EDAAFSPADRIGQLTMRNLDISDTRAKLSIYRRSGLLNASEGPAVLQIEGEKDGKKG"
     gene            12876..13724
                     /locus_tag="PK264_10915"
     CDS             12876..13724
                     /locus_tag="PK264_10915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD76435.1"
                     /translation="MNGVRLYFDVDGLELVPDGPNMRERPTIILLHGGPGFDHTLYKP
                     AFSALTDVARIVYLDHRGNGRSDGRDPADWTLAQWGDDVKGFCDVLGIEKPIVYGASF
                     GGMVALSYATRHPSHPGKLILVSTEAAGYSHIDRRIAMFERLGGPEVGALARRRFREG
                     HADPAVTDAWMRLALPHYTRTPADPDAMARGIRNVDVLRWFTRPGGEAQSFNFFPDLS
                     RIICPTLVLGGDDDPMLPIECQADIAAALPSHLVRFERFANCGHSVVPDMAEKAFSVI
                     RQFIRH"
     gene            13819..>14436
                     /locus_tag="PK264_10920"
     CDS             13819..>14436
                     /locus_tag="PK264_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76436.1"
                     /translation="MGGKFELMKIARIPVLVDVTFIILIVLWGSGYLQAGNTQMLSAG
                     VVVVIGLALSILIHEFAHAFSGRYFGTRVAAVELNGLGGLCYWASSLPRNAWSRIAIS
                     LAGPASNLALWFIFDQLFKLAAASTVRNPLLLHVLSMLSIANWYLFLFNMLPSFPLDG
                     GRALEDFLGQYIGGYKATRFVASLGLLVAFGVGAWALMNNYGLWTL"
BASE COUNT         2241 a         4538 c         4895 g         2663 t
ORIGIN      
        1 acattgcgag tgtccttatc gatcgcgaag ttcgtccgag ctctcggcac gtggtgatga
       61 acttcgcgat cgggacacta gccgccacga gcaagcgttc cttcgcggac gtcgatgagg
      121 gcacaaatcg ttcctggtac ggtccgatca cgccacatgg ttccacgacg ccgcgtcgcg
      181 cctttgctat gcttccggcg agatgctgac aattcgggag ggtgcgacca tgactgatca
      241 caatcgccag ccggccgtga agctcgacgc gccgggccgc ggccggatct acaacagtat
      301 cgtcgagacg atcggcaata cgccgctcgt gcgcgtgccc gggctcacgg ccgagaaggg
      361 cctcaaggcg gatctttgcc tgaagctcga gttcttcaat ccgatcgcga gcgtgaagga
      421 ccgcatcggc gtgaacatga tactcgagct cgaggcggcg ggccgcatca agcccggcgc
      481 cacgctgatc gagccaacat cgggcaatac gggcatcggc cttgctttcg tcgccgcctc
      541 gcggggctac aagctcatcc tgacgatgcc cgattcgatg tcgatcgagc ggcgcaagat
      601 gctcgcttac ctcggcgccc ggctcgagct cacgccgcgt gagaagggca tcaagggctc
      661 catcgcgcgg gcggaggaac ttctggcgga aatcccgggc tccgtcatcc cgagccagtt
      721 ctcgaacccg gccaatcccg acatccacaa gcgcacgact gcggaggaga tctggcgcga
      781 cacgggcggc gaggtcgacg ccatcatctc gggtgtcggt accggcggca ccatcaccgg
      841 cctcgggcag gtcctgaagc cacgccgggc gggcctcaag atcatcgccg tcgagccgga
      901 gctgtcgccg gtgctctcgg gtggcacgca cagcccgcac ccgatccagg gaatcggcgc
      961 gggcttcgtc cccgagatcc tcgaccgctc ggtgatcgac gaagtcctga ccgtctcggg
     1021 caaggacgcg atcgacacgg cgcggctact cgccgccctg gacggcattc ctgtcggcat
     1081 ctcgtcgggt gcggcggtcg cagtcgcgct gcgcgtcgcg ggccgcccgg agatggcggg
     1141 gaagcgcatt gtcacgatca ttccctcgtt tgccgagcgc tacctctcga cggtgctatt
     1201 cgaagggatc tgacggccgc ctgccggcgc atcagtactg cggcggcagg ctctgggagt
     1261 gtgcgtcgta ggcgaggttc gagaagcgcg tgaagttgcc ctcgaaggcg agcgtgacgg
     1321 tgccgatggg gccgtgacgc tgcttgccga tgatgacctc ggccttgccg tgcacgcggt
     1381 ccatgtttgc ctgccaggtg aggaactcgg gagtgccttc ggccggcttg gtgcgctcga
     1441 cgtagtactc ctcgcggaac acgaacatca cgacgtcggc gtcctgctcg atcgagcccg
     1501 attcgcgaag gtcggaaagc tgcgggcgct tgtcctcgcg cgtctcgacc tgtcgcgaga
     1561 gctgcgagag agcgatgatc ggcacgttga gttccttggc gagcgccttc aagccggtcg
     1621 tgatctccga cacttcctgc acgcggctgt cggaggagcg cttgcccgag cccgagagca
     1681 gctgaaggta gtcgatgatg aggaggccga gcccgcgctg gcgtttgagc ttgcgggcgc
     1741 gggcggcgag ctgcgctata gtgatgccgc cggtctggtc gatgtagagc ggcacgctcg
     1801 acatctcctg agaggcgcgc acgaggcggc cgaagtcggc ctcgtcgatc atgccgcggc
     1861 ggatcttctc cgacgagatc tcggattgct cggaaagaat gcgggtggcg agctgctcgg
     1921 ccgacatttc gagcgagaag aagccgacca ccgcgccgtc gacgaccttc tccgtgccgt
     1981 cggtctgctt ttctgtcttg aaggccttgg cgatgttgaa ggcgatgttg gtcgcgagcg
     2041 ccgtcttgcc catggaagga cggccagcga ggacgatgag gtccgacgac tggagcccgc
     2101 ccatcttgtt gtcgaggtcg gcgaggccgg tcgagatgcc ggagaggccg cccgcccgct
     2161 tgaaggcgtt gttcgccatc tcgatagcgc cggtcagcgc ctcgcggaag ccgaggaagc
     2221 cctggccgaa cttgccggtc tcggcaaggg aaaagaggcg cgactcggcc tcctcgatct
     2281 gcaccttggg cggatggtcg acaggggtgt cgaacgcctg gttcaccatc tcctcgccga
     2341 tggtgatgag ctggcgtcgg atcgccagat catagatcgt ctggccgtag tcgcgggcgt
     2401 tgatgatcgt cgtcgcactc gccgcgagct tgccgaggta gagcggcacc gtgaggcccg
     2461 gctcgatcgg ctcctcgttc tcgaaaaagg tgcgcagcgt gatcggcgtc gcctgcttgc
     2521 cggccgcgat cagcttggcg gcggtctcga agatgcgggc gtgcagggga tcgaagaagt
     2581 gctcggcggt gaggaacgag gacacgcgat cgtgcgactc gttgttgatg aagatcgcac
     2641 cgaggagcgc ctgctcggcc tcgatgttgt gcggggcaac gcggaacggc aggccgtcag
     2701 cggcggtctc ggtgggtgct ggggcggcgg cgcgcgcgcg gcgctggact tcggtcatgg
     2761 tctgccatcg ctgtgtgtga cgtgatcggc ggaacataat cgatgtgacc tgtcgccgtg
     2821 gcgcgggtca ttggtgttat ccgacctgtc cacatgcgac atcggcgagc ggtgccaact
     2881 tgcgcttgcc ttgcggcgtg tcggccgcgt cggtgattga ttcgcgcgtc tggtctcttt
     2941 acgcagcggg acaggatgct tatttcacat tgctttgttg ccgcacgccg ctgcggcggg
     3001 ggccgcctgg agggaacgac atggagcgct tctcgaacgc agtgacgtat ctcggcagcc
     3061 accggcccga gcgccccgtg ttcggcatgc ggccggaggc ggcagggcgt gccgcgcgct
     3121 ggttcctcca gaactttcca ggcgatgtcg cctatgccta caaggcgaac agccatccac
     3181 tgatcatcgg atcactctac ggggctggca tccggcattt cgacgtcgct tcgctgcctg
     3241 agctcgagga cgcggcgacc attccgggcg cgatcctgca tttcatgcac ccggttaagt
     3301 cgcgcaatgc catccggcgt gcctactcgc tcggcgtgcg cagcttctca ctcgacagcg
     3361 aggaggagct cgacaagatc gtcgaggaga cgaatggggc ccgcgatctc gccctctcgg
     3421 tgcgtctggc cgtgccagcg aagaacagcc gactgccgct cgagcgcaag ttcggcatcg
     3481 ccatgagcaa ggcggcgccg ctgctgctgc gcacgcgaca ggccgcggcc gagctcggcc
     3541 tcacctttca cgtcggctcg cagaccgtga cgccggacgc ctacacgctg gcgctcgccg
     3601 agatcggcaa gctcatcaag cgggccggcg tcgtcgtcga caagatcgac gtcggcggcg
     3661 gatttccgtc gcgctactcc gaggaggcgc cgacgccgct tcagcacttc gtcgatgcga
     3721 tcaagctggg attcgagcgg ctccccgtga aggagacctg caagctcatg tgcgagccgg
     3781 gccgcgcgct ggtcgccgag gccgagagtc tgatcgtgcg catcgatgcg cgccgcggca
     3841 acgagctctt catcaacgat ggcggctacg gcgcgctgtt cgacgcggcg cacctaggct
     3901 ttgccttccc ggtgcgactg gtgaccggcg gcccgcgcga tgcggccgac ctgatgccgt
     3961 ttgagctctg ggggccgaca tgtgattcgg tcgaccgcat gaaggggccc ttcatgctcc
     4021 ccgataacgt gcgcgagggt gactacctcg agctcggcaa tcttggcgcc tacggacgcg
     4081 ccattgcggg caagttcaac ggctacggcc agtacgacga ggtgttgctc ggcgacgagc
     4141 cgatgtactc catgtatcgc ggctcgaatg cgactgccgt cccggacccc gagggggtcc
     4201 ggtagcggcg acatcgcatc acgacttggt cctcggattg gcccgccggc gtcgcgacag
     4261 catattgagg ccctcgacga aggccgagaa cagcatggcg gcgtagatgt agcccttcgg
     4321 gatcttggcg ccgaagcctt ccgcgatcag cacggcgccg atcatcagca ggaagccgag
     4381 cgccagcatg acaatggtcg ggttggcatt cacgaagttc gcgagagggg ttgccgcaag
     4441 catcatgacg gcgacggcga cgagcacggc gattaccatg atcggcacat gctcggtcat
     4501 gccgacggcc gtgatgatgc tgtcgatcga gaacacgagg tcgagcagca ggatctgcgt
     4561 gatcgcggag gcaaacgtca ggctcgcctg accggtatcg aacacatcct ggttggggtg
     4621 cgggtcgaca cggtggtgga tctccttcgt cgctttccag acgaggaaca gcccgcccgc
     4681 aatcaggatg atgtcgcgcc acgagatacc gtgcccgaac agtgtgaata gcggcgccgt
     4741 cagctggacg atccatgcga tcgtcccgag caggacgagc cgcatgatga gcgcgaggct
     4801 gatgccgatg cgccgcgcct tggcgcgata ctgctcggga agcttgttcg tcaggatggc
     4861 gatgaagatc agattgtcga tgccgagcac cacctccatc gcgatcagcg tggcgagggc
     4921 cgcccaggcg gcggggtctt gggcaagcgt catcaggttc gacatggagc cttgcaagat
     4981 agctggtggc ctgagctgtg gttttggcct agttgtcgct ccgagcaaag cgctttcgcg
     5041 cactggacgg ggacaggggg aacgggtgga atgattcgga tcgagcatgt cgaaccgctc
     5101 aatgcgtcac gatcgaaatt ccggccgtcc ggcatcggct tttacgtact gcccgaacag
     5161 gtgacgctca ttcgcccgac ccgcgacccg aatgtctcgg aggtgcggct ccagggaggc
     5221 gccgtctact tcgcgggcgc gtcccccgca gcgctcgagc aggcgctgct cgacaacgcc
     5281 cgacgctaga ggccatggcg agcgacacat cgggacagcg gccgcgccag ggcctgctgc
     5341 tcggcctttc cgtattggcg ataggaacgt tggcggcgcc cctcgatacg tcactcaaca
     5401 ttgcgctgcc cgatatctcg cgagcgttcg caagcggtgt tgccgatgtg cgctgggtcg
     5461 tcatcggcta cgtaatgact tatgccagcc tgatgctggt attcggccgc ctcggcgacg
     5521 tcgccggatt catgatcgtg ttccgatgcg ggcgatcaac gcggtgggtc tggtggcctg
     5581 ctcgcttgcg acgacgctgc cgatgctggt cagcgcccgc gtccttcagg gggtcggagc
     5641 ggccctgacg tttgcctgtg ggccggcaat cgcgacggcc ctcggcggag aggggcgccg
     5701 ggcagaaatg ctctcattcc atgcgatggt aatcgcgatt ggctcggccg ccgggccgct
     5761 cgtcggcgga atgctggtcg acttatgggg ctggccggct gtgttttggg cgagggcgcc
     5821 attgccgttg gcagccttgc tgctttcttc ggcgatgtcg ccgttatcgc aagctccgcc
     5881 acgtggtggc atggattggc tgaacggcat gcttgtcgtg ttggccatgg ctggtgccgg
     5941 atcgacgctc gggttcgctg cgggtacggc gacgccgctc gtcgcactcc tggccgctgt
     6001 ggcaattgtt gcctggtggg tgcaaggcca aagatctgcc gcgcccgtgc tcgacccggc
     6061 tcattttcgg cagacggagt tcactgtatt gaacctgtcg accatcgcga tcaacctcgt
     6121 cgcctttgcc gtgctcctga tcgcccccta ttacctcgtg cgcatggttg gactgtcgaa
     6181 ccgcgatgcc ggcctcgtcc tctcgttgcc attttttggc atgctcgcgg gagcttggct
     6241 cgttccacgg ctgacgacct ccttgaacgc gggccgattg gcgttcgccg gcctttgctc
     6301 ggttgccgtc atgcagggcg cgattggtgc cctcgaggcg acaagcccga tggcattgat
     6361 catggcggca ttcgggctgc atggcatggg ccttggcgta tttcaggtag cggctgcgac
     6421 catcgttaca gggactctgc cactggagca gcgcggcgtg gctggaagcc tcggcatcgt
     6481 catgcggaca ataggcgtgg ttgcgggtgc attcgtgctg gccacccttt ttgatacgat
     6541 gagtggcacc gacgccgtcg gcgagaggga gcggatcgag gccggtgtcg cggccttcaa
     6601 ggcaacattc atctattcca gccttgcatt ggccatcttc attctggcga cactggcctg
     6661 gccgcggacg tggcactggg agctggacga cgcggcgcgt cggtgacacg cgtccaaagg
     6721 cgaaacattt gcgcggagtg cctgtggctc tgcgaggacg aggcgtggta gatgaatagt
     6781 ccagtctgcg cttcagggcc gaacatggtc gatcggacag tgctgatcat gcggaagtgt
     6841 cgctcggcag cgctgggccg acgtcttgcg gggtctgccg cgacgttggc gctcgtcctc
     6901 ggctcggcag cggttgcgca gcagccccac gccggtttga acgggccgcc acccgcggca
     6961 tccggcccgg gtcccagcgg gcccagctcc ggcgcgacgg tcacctatca ggtgcgctcg
     7021 aagaacgggc acttctacgt gccgctgacg attgccagtc gtgccgtcac catgctggtc
     7081 gatacaggcg cgacgacagt cgtgctgtcc gccgccgatg ccgagcggct cggcatcgat
     7141 gtccgccggc tgcgctacga tacggcaacg gcgactgcgg ccggccgcct gatggtcgcc
     7201 agcatcgagc tccacgatgt gcgcatcggg cgcatctacg tcggccgggt cgcggcgacc
     7261 gtgctgccgg agaactccgg caagcagtcc ctgcttggca tgagcgtcat ccagaagctc
     7321 ggccgcgtga cggtcgaggg cgaatcgctg accatcgtcg gcgtcatcat ccccggcgcg
     7381 ctggtcatgt tcagtgtcgc gggcgtaccg ccggcgtcga atgcaaggac gccgccgctg
     7441 cgtccggcgc cgcccccgac atcggtcagc ggggcacgtc tcggatgggg cgcgctcgcc
     7501 ctgtcggcgg acgcctatgg ctggtcgcgc ggttatgcca cccgttccga ggccgaacgc
     7561 gtggccctcg acaactgccg caagcatgcc aagcgcgacc aatgcgacgt ccgcgctttc
     7621 gacggctcgt tgtgtgcggc agtcgccacc tggtcgttca tggagggctc ggagcgccgt
     7681 tttggcaaca tcggcaactt cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnncaccgaa tgccgtccgc cgtatgcatc acgccgcggc gactcgtact cgtcgcggcc
     7861 gtcgccctca tcgatgtcga taatcgcgtg ctgatcgcgc agcgcccacc gggaaaggcg
     7921 atggcgggcc tctgggagtt cccgggcggc aaggtcgatc cgggcgagac gccggaagag
     7981 gcactcgtgc gcgagatcac cgaggagctt ggcatcgaag tttgcgagcc ctgcctcgcg
     8041 ccgctcacgt ttgcgagcca tgcctatccc gatttccacc tcctgatgcc gctcttcatc
     8101 tgccgcaact gggacggcga gatcagtgcg cgcgaaggcc agcaactcaa gtgggtgcgg
     8161 gccatgcgcc tgtccgacta cccgatgccg cccgccgacc tgccgctgct gccggcgctg
     8221 cgcgacctgc tcggctgagg cgcagatcga gtgagccggc ctcagggccg ctccgagagc
     8281 gcaaggcgga cgccgacgac gatgaacagg ccgccgatcg cccgctcgag ccagaccttg
     8341 atgcggacgt aggcacgcga ttccgcgagc tgcccggcaa accacgcgac cgcgaggttc
     8401 caagccgttc cgaccgcgtt gaacagcaac ccgagcgtga cgaaggccag caccttggac
     8461 ggcgtggccg gatcgacgaa ctggggaagg aacgccagga agaaaatcgc aaccttcgga
     8521 ttgagcgcat tggtgagcgc gccctgcaag aatgcctgcc aaaacgttac cggttcctct
     8581 cgctccagac cggcactgcc cgccgtccct ttgaaggtcg cgcgcaccat ctggacgccg
     8641 agatagacga gatagagggc gccgacccac ttcacgatgg tgaaggccgt cgccgaggtc
     8701 acgagcacgg ccgaaagccc gatcgccgcg gcggcgatgt gggcgaacgc gccggcgccg
     8761 acgcccaggg cagcgcccat accgacacgc gtcccatgtt gcgtgctgcg cgagaggatc
     8821 agcgcaatgt ccggcccagg cgtgatgttg agcagcagcc ccgcgaggat gaaaagccag
     8881 agatcctcta gtcccatggg tcgcgcccgc cgccgagaag ggttgccgtg tctggcgcga
     8941 gtaacagcgc ggcgcagtcg tgtcgcctgg acaaagtgcg ccgcggtgcg gtcggttcat
     9001 ggaggaggcc cagcggaccc tcgatggacc gcagcggtgg ccgacacgaa acgggtcgca
     9061 tccgcgagga gcagtcgcta acgtcccggc acttctaagc gggggcgagc atgtcggcca
     9121 aggcatcgac gacgattgcg gcgagcgact ggggcctgct cgcgatcctc tccgtgctct
     9181 ggggcggctc gttcttcttc gccggcgtcg cggtgcgcga gctgccgccg ctctcgatcg
     9241 tgctcgctcg tgtcgcgatc gcagcccttc tgctcatccc cctgcactgg ctgctcatcg
     9301 gcgccctacc tcgtgcgcgg tccgcctggc tcgcatttct cgtgatggcg gtactcaaca
     9361 acgtgatccc gttctcgctg atcgtgacgg ggcagaagga gatcgcgagc gggcttgcct
     9421 cggtgctcaa tgcgacgacg ccgctcttcg cggtcatcgt tatggcggcg ttcggggacg
     9481 agaagctcat cgcgcggaaa gtagcgggcg tgattgtcgg cctcgccggc gtcgcgatcc
     9541 tgcgcgggac gacgcccatg tcgctcggcg atggcgcgac gctcggcgtg gcgctctgcc
     9601 tcgccggggc gctctcgtac gggtttgccg ggctctgggg gcggcggcat cttgccggcg
     9661 tgccgccgct cacctccgcg acgtgccagc tcatctgctc gaccgcggtt atggctgcgc
     9721 tcgcgagcct ggtcgacgcg ccgtggcgtc ttgccgtccc aagtgtcgcg acgctctggt
     9781 cgctcctcgg gctcgcggcg ctctcgacgg cactcgccta tgtcgtcttc tttcagatcc
     9841 tcgtgcgcgc gggcgcgacg aatgttatgc tcgtcacgct gctcatcccg ctgacgtcga
     9901 tcctgctcgg ccacctcgtg ctggacgagg cgatcaggcc tcgcgagatc gtcggcgcgc
     9961 tggtcattgc gagctcgctg ctgatcgtgg acggccggat tttcagcgcg ctgcggcgcg
    10021 accgcgatac gtccgcggcg ccgtgatgcc acgccttttc agccgaggac gaccggcagc
    10081 gacttgatcc cccgcaggat gagcgagtca ctccactcgg gcgcgcccga gccggcaagc
    10141 ctgacgcgcg ggaagcgctc ggcgaggcgc ctcaccgcga tctgcgcctc gaggcgggcg
    10201 agcggcgcgc cgaggcagaa atggatgccg tgtccgaacg tcaggtgagc gttgggcgta
    10261 cgggcgatgt cgaagcggtc agggtcgggg aactgggcgc cgtcgcggtt ggcggcgttg
    10321 gtgaaggcga agacgcgctg gcccttcttc agcaagcgcc cgccgaactc gatgtcgtcg
    10381 acgacgacgc gggcgagggc actgctcggc ccgtcgtagc gcagccactc ctcgatcgcc
    10441 ggctcgatca gccccgtatc ggcaacgagg cgctcccact gcccgcgaaa tcgcatggcg
    10501 tggtagaggc cgttgccgag caggttcgtc gtcgtctcgt gcccggcgaa gagtaggagg
    10561 atcgatgtgg cgatcacctc atccgaggtc aaagcctcct tctcgtcgcg ggcgatcacc
    10621 atctggctga tgatgtcgtc acgcggcccg gccgtgcgat cggcgacgat gccgcggaag
    10681 tagtcagcca tggcgcgggc gccccgctcg gcccgatcgt atttgtcggg cgtcgcgcgc
    10741 gccgtgccga tgaaaagggc gatatcgtcc gaccagagct tgacgcgctt aaggtcgccg
    10801 cgtggcacgc cgaggaggtc catgatcacg gtcgcgggga gcgcgtatgc gaagtcggcg
    10861 atccagtcgg ctgtgtcgcc cgcacgcgcc ttggccgcca tgtcgtcgaa gagcatgtcg
    10921 acgaggtcgc tgatgcgcgg ctttagagag tcgagcgagc gtgaggtgaa ggccttcgtg
    10981 aagaggcggc ggaggcgcgt gtggtctggt ggatcgcgga acaccatcca gccgccgagg
    11041 taggtcatca ggttggcgta ggtcgagcgg cgggcctcgg gggccgcggc gaagaacgga
    11101 gcgagccggt tggccgatac ctcggcgttg ttgagcgaga tcgcctttac gtcgtcatag
    11161 cgcgtgacga cccacgcctt catcgcctcc gaccagtgcg ccgggtcctc cgcgcggagc
    11221 cgggcaaaga tcgcaaacgg gtcggcattg gtggcagggt cggcgaagtc gatcgcgagc
    11281 atcggggcgt ctcgtcgaag tgctttgttg cggccgggag cgctaatcag cccttcttgc
    11341 cgtccttctc gccctcgatc tgaaggacgg cggggccttc actcgcgttg agcagaccgg
    11401 accggcggta gatcgagagc ttggcgcgcg tatccgagat gtcgaggttg cgcatggtga
    11461 gctggccgat gcggtcggcc ggcgagaagg ccgcgtcctc gacgcgctcc atcgagaggc
    11521 gctcgggctg gtacgtgagg ttcgggctct cggtgttgag gatcgagtag tcgttgccgc
    11581 ggcggagctc gagcgtcacc tcgcccgtca ccgcgcgcgc gacccagcgt tgcgccgtct
    11641 cgcgtagcat cagcgcctgg ctgtcgaacc agcggccctg gtagaggagg cggccgagct
    11701 tcatgccgga gatgcggtac tgctcgatcg tgtcctcgtt gtggatgccg gtgacgagcc
    11761 gctcgtaggc gatgtgcagg agcgccatgc cgggcgcctc atagatgccg cggctcttcg
    11821 cctcgatgat gcggttctcg atctggtcgc acatgccgag gccatggcgg ccgccgatgg
    11881 cattggcctc ggtcatcagc acgacgggat cggaaaattc ctggccgttg agcgcgaccg
    11941 gcaagccttc gtgaaagcgc actttcacag tctcgggctt cacgtcgcag tcctcgcgcc
    12001 agaaggcgac gcccatgatc ggatttacga tgcggatcga gctcgagagc tcctcgaggt
    12061 ccttcgcctc gtgggtggcg ccaagcaggt tgctgtcggt cgagtaggcc ttctccgcgc
    12121 tcatcttgta gccgaagccg tgcttcgtca tgaactcgga catctccttg cgcccgccga
    12181 gctcgtcgat gaaggcctgg tcgagccagg gcttgtagat cctgaggctc ggattggtga
    12241 tgaggccgta gcggtagaag cgctcgatgt cgttcccctt gaaggtcgag ccgtcgcccc
    12301 agatgttgac gtcgtcctcg cgcatcgcgg tgacgagcat ggtgccggtg acggcacggc
    12361 cgagcggcgt cgtattgaaa taggtgatgc cgcccgtcga gatgtggaag gctccggcct
    12421 ggatcgcggc gatgccctca tgcacgagct gtgcacggca atcgacgagc cgggccttct
    12481 cggcgccgta gtccatggct ttccgcggga tcgcctcgta gtcggtctcg tcgggctggc
    12541 cgaggttcgc cgtgtaggcg aatgggcgcg cacccttgag tttcatccac agcagtgccg
    12601 cgctggtatc gaggccgccg gagaaggcga tgccgacctt ctgccccttg ggcaggctct
    12661 gcaggatggt gctcatgggt gttctctggc ttgatcttag cgcgcgttcc ggagttgatc
    12721 tcaaattttc cgcgccggcg caaacgatgc gcgtgcaagc catacggtgt caggactgcc
    12781 gcactcggat ggacggcggg ctgggactgt cgctacactt gtgtcacaag atgggcgaca
    12841 atgcctgcaa gaggccgtaa tcgatgcatg tccatgtgaa cggagtcagg ctctacttcg
    12901 acgtggacgg gctcgagttg gtgccagacg ggcccaacat gcgggagagg ccgacgatca
    12961 tcctgctgca cggagggccc ggcttcgatc atacgctcta caagcctgcc ttctcggctc
    13021 tcaccgacgt cgcccgcatt gtctatctcg accatcgcgg taatggccgc agcgatggaa
    13081 gggatccggc agactggacc cttgctcaat ggggtgatga cgtcaaaggt ttctgtgatg
    13141 tgctcggcat cgagaagccg atcgtctatg gggcctcgtt tggcggcatg gtcgcgctgt
    13201 cctatgcaac gcggcatccg agtcatcccg gcaagctgat cctggtcagc acagaggccg
    13261 ccggttactc gcacatcgac cgccgcatcg cgatgttcga gcggctcggc gggcccgagg
    13321 tcggggcgct cgcccggcgc cggttccgcg aaggccacgc cgaccctgcc gtcacggacg
    13381 cctggatgag gctcgccctc ccccactaca cacggacgcc cgcggatccg gatgccatgg
    13441 cgcgaggcat ccgcaacgtg gacgtcctgc ggtggttcac gcggcccggc ggcgaagccc
    13501 agtcgttcaa tttttttcca gacctctcga ggatcatctg tccgacactg gtgctcggcg
    13561 gagacgacga cccgatgctg ccgatcgaat gccaggcaga catcgccgcc gctctgccga
    13621 gccacctcgt gcgcttcgag cgctttgcga actgcggcca ttccgttgtt cccgacatgg
    13681 ccgagaaggc attttccgtg atcaggcagt tcatcaggca ttgacccggc ttcatgtgga
    13741 tgctcgaagt cgctctactc gggcgcgatc gctgagccgg cggtcgatcg catcgggtcc
    13801 gaaagcacgg ggacgggaat gggcggcaag ttcgagctga tgaagatcgc gcgcatcccg
    13861 gtgctcgtcg acgtgacgtt tatcatcctc atcgtgctct ggggctcggg ctacctgcag
    13921 gccggcaata cgcagatgtt gtcggcgggt gtcgtggtgg tgatcggtct cgcgctctcg
    13981 atcctcatcc acgagttcgc gcacgccttc tctggtcgct atttcgggac gcgcgtcgca
    14041 gccgtggagc tcaatggcct cggcggcctc tgctattggg catccagcct gccgcgcaat
    14101 gcctggtccc ggatcgccat ctcgctggcg ggtccggcct ccaacctcgc gctctggttc
    14161 atcttcgatc agctcttcaa gctggccgcc gcgtccactg tccgcaatcc gcttctgctg
    14221 cacgttcttt cgatgttgtc gatcgccaac tggtatctct tcctcttcaa tatgctgccg
    14281 tcgtttccgc tcgacggcgg acgtgcgctc gaggatttcc ttggccagta catcggcggc
    14341 tacaaggcga cgcggtttgt cgcctcgctt ggccttctgg tcgcattcgg cgtcggcgcc
    14401 tgggccttga tgaacaacta cggcttgtgg acgttg
//
LOCUS       DAORNH010000090        14322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3201_length_14322_cov_2.699797, whole genome shotgun sequence.
ACCESSION   DAORNH010000090 DAORNH010000000
VERSION     DAORNH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3201_length_14322_cov_2.699797"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..162)
                     /locus_tag="PK264_10925"
     tRNA            complement(86..162)
                     /locus_tag="PK264_10925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Pro,seq:tgg)
     gene            382..456
                     /locus_tag="PK264_10930"
     tRNA            382..456
                     /locus_tag="PK264_10930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Glu,seq:ttc)
     assembly_gap    1274..1286
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1494..>1661)
                     /locus_tag="PK264_10935"
     CDS             complement(1494..>1661)
                     /locus_tag="PK264_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD76437.1"
                     /translation="AKGLRHAFGLHAIRCGVPLNLVQRWLGHASITTTVIYLQALGPE
                     EREFAARMWTN"
     assembly_gap    1662..1706
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1707..2072)
                     /locus_tag="PK264_10940"
     CDS             complement(<1707..2072)
                     /locus_tag="PK264_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRD76438.1"
                     /translation="MTATLYTHDGCRKYLNAVERARFLDVAAGEPASIHALCQTLAHT
                     GCRLSEALALTPRAIEADSGFVAIRSLKKRSRNFIIREVPVPPAMIEQIDRTFHLADH
                     DPAARLWAMSRITAWSVVKD"
     gene            2389..3189
                     /locus_tag="PK264_10945"
     CDS             2389..3189
                     /locus_tag="PK264_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76439.1"
                     /translation="MAMRSGTAVLVATILAVSLSGIRTAHAQAKPDVVRPEITDIRGQ
                     LRMHDGAVLVGQHGLWGSREIQPDQYRLNLLLILTANPNLLTDRNWTSRFVGILPDAE
                     AKRYADGRIDRWSWRGGDEFQREAMRRAFIEKEGERLKRLIPPIPFELLDVRGADLES
                     YNFDKGGYILGNGLQSETLVGLKLPFRVPSFWAVPPDKAQAILAQVQATAPHKNHSIW
                     RQAAFASRLRVTGVRWERDNLLIDTALVKIEMYAGPDLKTKLGDVPTP"
     gene            3265..4158
                     /locus_tag="PK264_10950"
     CDS             3265..4158
                     /locus_tag="PK264_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76440.1"
                     /translation="MYQLDFAAFMIGAVVGLALAIIAILKIRTNMVESTIEGRPDKSV
                     GRVVEIVWTISLLLAFAHVVHHLLLFFCSFNPSYLLRLQPDPSQPWILPEAWQMPFYL
                     LVLTWLMSPLAVASIVGQISCGVRLAPETPHHASVGRKVAFAVLTLLAVVALLPLLLT
                     VPVIWEQVLLPVIRAVLGIALLIIVAAAFSGKKTDGQPASGGKSSAVAAKAPPKKQAS
                     DKWERCTTCHGRGSIDCNVCGNMGKISCMSCGGSGSQWRSSGAVTCGTCSGSGRQGCG
                     SCGGYRRCSSCSGHGGRYVRR"
     gene            complement(4215..4565)
                     /locus_tag="PK264_10955"
     CDS             complement(4215..4565)
                     /locus_tag="PK264_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76441.1"
                     /translation="MVRTARKSSSKPARRTRAKAAPKTATHKLVWRHASCRVRHTRDY
                     ISKGWSHIELTVISPRGAPLPITSTGYLSHFLDEADLAAAGGPFLAWLDREAASKAWH
                     RAEFAWRQGDLFAT"
     gene            complement(4571..5470)
                     /locus_tag="PK264_10960"
     CDS             complement(4571..5470)
                     /locus_tag="PK264_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HRD76442.1"
                     /translation="MTAPSLKPRRDLHAEITGRIIAAIEKDPGHPQMPWRRSGAPLWL
                     PENAHTSRLYNGINVVALWAAAEMRGLTVPVWATYRQWAEKGCQVRKGAKAELVIFYK
                     EYDAEPDPDDAEDDGKRRVARASYVFNAADVDGFTAPAAPERLGPIARLESADRFISA
                     TGARIEHGGERAFYRPSADLITMPDEALFCGTETMTRDESWYAVVLHELTHWTAPKHR
                     CDRDLSRRFDKCEVAAEELVAEIGSALLCAELGVTQDTRPDHAQYIAHWLELLKRDPK
                     AIFTAAAKASQAVAFLKSLQPKE"
     gene            6039..6368
                     /locus_tag="PK264_10965"
     CDS             6039..6368
                     /locus_tag="PK264_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76443.1"
                     /translation="MIDVELSEDADDEDFDPMSKAEMRIEDAIHAFESTPLNLRRIRS
                     WLRGRVSAAAHEQLLKSDFRALMSEEEYASVMASARKNFCPTITRQWLGETKPESLRA
                     TTAPKAK"
     gene            6421..6798
                     /locus_tag="PK264_10970"
     CDS             6421..6798
                     /locus_tag="PK264_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRD76444.1"
                     /translation="MSEAMTRRTLIAGSTAIGAALTCGIEPTNEVARVLAALPVVTGR
                     IKWFDASKGYGFIVPDKAGADILLHVVCVRASGFENVREGSRVRVKVIERPKGRQAAQ
                     ILAMDTSTAVANPEIRTHRIISA"
     gene            complement(6788..7870)
                     /locus_tag="PK264_10975"
     CDS             complement(6788..7870)
                     /locus_tag="PK264_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRD76445.1"
                     /translation="MAEALRMTLIPWLGVCLGIGAMLFTWWLITYFRRPSFADLHEVA
                     VPVACGAVFICLFVMWYTTWRAGPASAKSCQLGYLLTGRWNEIDTQLLSQEALAWLVR
                     VFFLPLNFSESARVMSGFRIQPEHYFDRPVMALHADLLMFIYGMIVTAIIPGYLFASR
                     LFSNEVRLVDRTLSAWVVTLWSYAPFLVNGAFFYYRAENWQSQFSANGLVLCVVGFCI
                     LLFELVHYWGEAQMGIRASNLSHRGIITTGPFRYTRHPIYLSKCIGWFFIWMPVTAGE
                     NWVECVRLTVLFVMACSVYFARSWVEERLLASDKDYVTYALWVDRHGLLARLGRHIPA
                     LTFEWRYRRWLKDGAIISDGLSPATR"
     gene            complement(8522..9304)
                     /gene="istB"
                     /locus_tag="PK264_10980"
     CDS             complement(8522..9304)
                     /gene="istB"
                     /locus_tag="PK264_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HRD76446.1"
                     /translation="MSDTPQVLLAHHLKALKLPTFLREYDRQARLCAAEGVDYPRYLL
                     RLAEQELIDRDRRMVDRRIKAAKFPAVKTLDSFDFEALPSLNKALVMELARSEYVERR
                     DNIIALGNSGTGKSHVAIGLGLAACQRGLSVGFTAAAALVHEMLEARDEKRLLRIQRQ
                     LAAYKLLIIDELGYVPLSQTGAELLFEVFSQRYERASTIVTSNLPFEEWTSVFGSERL
                     TGALLDRLTHHVHILEMNGESYRLAHSKRRRSKAKSDEPSQK"
     gene            complement(9301..10851)
                     /gene="istA"
                     /locus_tag="PK264_10985"
     CDS             complement(9301..10851)
                     /gene="istA"
                     /locus_tag="PK264_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043767605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRD76447.1"
                     /translation="MKRVELYAQVRYAVQIEGLSHREAARRYGIDPKTVAKMMRFSVP
                     PGYRRTKPAVKPKLDPFIGFIDKILADDKDRPTKQQHTSKRIFERLRDEHGYSGGLTI
                     VKDYVAGSRQRSREMFVPLAHPPGHAQADFGEALAVIGGTKRKVHFFAFDLPHSDACF
                     VIAYPAETTEAFCDGHVKAFEFFGGVPRSILYDNTRIAVARILGDGTRQRTRTFSELQ
                     SHYLFQDRFGRPGKGNDKGKVEGLVGFARRNFMVPVPAFDCFETFNADLAGRCRKRLS
                     ERLRGHSETIGERLARDLDALRKPLPPPYDACEKVPTTVSSLSLVRYRGNDYSVPTEF
                     GHAQVLVRGYVHEVVIACGTEVIARHPRSYEREDFIFDPLHYLALIEKKINALDQAAP
                     LANWQLPEEFATLRRLLEARMGKPGKREFVQVLRLMETFRIDDVAAAVRDGIDRGAVG
                     FDAIKHLLLCRIERRPPRLDLSIYPYLPRATVRTTSARDYMGLLRAGCEPGCEWASEG
                     VSESGGAP"
     gene            complement(11420..13114)
                     /locus_tag="PK264_10990"
     CDS             complement(11420..13114)
                     /locus_tag="PK264_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017273616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRD76448.1"
                     /translation="MAPITPFVSLTRDDLLGVGVPEDWIGDIISATEDGFLELATHLP
                     GEASEALLEYVATGSLKKPAPIVVADPFAHPDALRRFRVVENVEELEQALSYPWDRWI
                     VFLHPSQRDLVERSFAGPARVAGSAGTGKTVVAIHRARRLARDSCSRVLLTTFSEPLA
                     FALERKLQILAGETSEVVPRITVASFDGIARELFQLVFGGKPAIASEELIRGQLVKAA
                     QAQAGSTLSERFLASEWAYVVDAWQIKSAEAYAEVPRLGRKNRMGAKQRERLWPIFQS
                     VLNDLAARGFETTASMFQKLTDHYGARSDKPFTHVVVDEAQDLGVPELRFLKSIVPSG
                     PDALFFAGDLGQRIFQQPFSWKGLGIDVQGRSLTLKVNYRTSHQIREAADRLFSGPVA
                     DVDGHQEDRRGTVSVFNGPVPTISLHDSEEGEISAVASFIEAAMRDGTKPEEIGLFVR
                     TREVLSRARAAAERLGLAVAELTPRKEGPVSAIRIGIMHLAKGLEFKAVAVMACDEAI
                     LPLQDRIAAVADEIELDDVYATERQLFYVACTRARDRLLISGLNPGSEFIGDLRNS"
     gene            13256..13585
                     /locus_tag="PK264_10995"
     CDS             13256..13585
                     /locus_tag="PK264_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76449.1"
                     /translation="MRLDASALGPLVGIVVVIDVGEQKALRCLMDDDTDVAVDAHGPE
                     VRVLRGIDPMKLKPWIGRIGLQIERRRFDRLLLVAREPAEGEREGIGDAKFHTCASLS
                     SLPRLGV"
     gene            complement(13908..>14322)
                     /locus_tag="PK264_11000"
     CDS             complement(13908..>14322)
                     /locus_tag="PK264_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD76450.1"
                     /translation="ADELKNAGKGNLFVIFGEPDIDILDQGDETIVVKVNGIDVFDPN
                     TGQIRSNDVKGIAAWFIDTDYNEESFFVRHAYFLGANDPYKSLKTALRAEINPDAWET
                     LYRDTSRPFPRPSTGRIAIKVINHFGDEVMKVFQV"
BASE COUNT         2783 a         4252 c         4571 g         2658 t
ORIGIN      
        1 caaacccctt cgaggtccgc aatgtattac ccatgtctcg ggaataaacc gtaacggatg
       61 tgtccggaac ggaccctaaa tttgttggtc ggggcagcag gattcgaacc tgcggccccc
      121 tgctcccaaa gcaggtgctc taccaggctg agctatgccc cggaccgcgc gccgcggcgc
      181 tgcacgttct taggtgatga cgcccctcga tgcaaggcga agtccggcgc ctcgggctgt
      241 gggccggtgc tgatcggctg cacggccgca gccggtcacc gcatccggcg agaactgcga
      301 gggcaacagt ttgcgggccg cctcgcgacc tgctagaagg acgccccggc cgcccatcgg
      361 ctggacatcc acggcgcgtc cgctcccttc gtctatcggt taggacgtca gattttcaat
      421 ctgaaaagac gggttcgact cccgtaggga gcgccaatcc tatgatgtga ctgaataaat
      481 cgattccaag tgagacactc ctcgtgacag gctcggcgcg acgcgggtgt ccttctgggc
      541 ggtggtttcg tgcgaagcgg cggcggtcgt cattgcggta ttcgcagtgt gatcgataag
      601 cggccgcgtc gcttccttgc ttcggccgat gggccggcgg gccccgcgca ggtcagcaaa
      661 aatcggaggt cgatcaggct gcccaaggac tcgcggtatt cggggtgctt gccggtcggc
      721 gtcttgcctc catcgatgca gtcctcctgc tcgcggtcct agggatcggc gtcggccttg
      781 aggcggacgg tgcggggcag cgcatgtcgg ctgcgggaaa tctggacccg ccacgtgccg
      841 aggggtagcg tctggaccga cgccacgaat gccttccaaa ggatcgcgcg ggttctatga
      901 acgttcacga ctttcggatg cgtgtggccg gtgccgtgaa caaagcgcgc gcattgtgcc
      961 caaaaagtgc cgagggagct tgcgcgcgtc tctatgcaca cgagtgcgca cgattgtgtc
     1021 acacccttgg tcgccaccaa agtatccatg caaaatcaaa tgcataagtt ctgcgtcgcg
     1081 ccgatgatgg attggagatt taatcaggcg atatcatgat cttgcgagtg cacatgtgca
     1141 attcgtgtgc agtgagccgc gcgaaacgat gcgaattttt gggtgacgat gaggagccaa
     1201 gtgcacacgc ccgcgatcga gcccaacgct gctatcgtct gctagcggat caatggattc
     1261 gcgcgaatga tcgnnnnnnn nnnnnngagc ctctcgatca tctgtacgcg atgcgacacg
     1321 cgaaaacggc gataccgacg ttgccaatcg cgaagccgcg cgtgtgcatg gaattcgaca
     1381 agcgatacag gtgcttgcag gagtaaaatc cgcgctacgg cgggcggatc ggccaacagg
     1441 tcgtactgac gccaggaact cagagtggtg ttaggctggc ttccccggct acatcaattg
     1501 gtccacatgc gcgcggcgaa ctcgcgctcc tccgggccga gcgcctgcag gtagatcacc
     1561 gtcgtagtga tgctggcgtg gccgagccat ctctgcacga ggttgagcgg tacgccgcac
     1621 cggatggcgt gaaggccgaa cgcatggcga agccctttgg cnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnatcc ttgaccacgc tccaggccgt gatccgcgac
     1741 atcgcccaca accgcgcggc gggatcgtgg tcggccagat gaaacgtccg gtcgatttgc
     1801 tcgatcatcg cgggcggcac gggcacctcg cgaatgatga agttgcggga tcgcttcttg
     1861 agcgaccgga tggcaacgaa gccggaatcg gcctcaatcg cgcgcggggt cagggcaaga
     1921 gcctcggaca gtcggcatcc ggtgtgcgcc aacgtctggc agagcgcatg gatagatgcc
     1981 ggttccccgg cggccacatc gaggaagcgc gcgcgctcga ccgcgttcag gtacttgcga
     2041 cagccgtcgt gtgtgtagag cgtggccgtc acgggaagag caggcatccg caggctccgg
     2101 gatgagttcc tgtgagccgt cacgacaacg gaattgccta ttctgtttcc tccggtcatc
     2161 ccctcaagcg gacaggcctt ttggaccgca tccataggca ggaagctaga agatcacacc
     2221 gccggatgtc aatgttttcc ctaacatcat caatgccttg aacagaatag gcaagactgt
     2281 tcggggcgga ccggcctgct tgtgaagctc ccgttggagc gaccgccgcc tgtcacccgg
     2341 ccatcctgcc gatctgtcct acccggttaa atcgggaggt attcagacat ggcgatgcgt
     2401 tccggcactg cggtgcttgt ggcgacgata ctggccgtca gcctctcggg cattcggacc
     2461 gcgcacgctc aggcaaagcc cgatgttgtg cgcccggaga tcaccgacat ccgtggccaa
     2521 ctgcgaatgc acgacggcgc agtgctggta ggccaacatg gcctttgggg aagccgcgaa
     2581 atccagccgg atcaataccg gctaaatctg ctgttgattc ttacggccaa cccaaacctc
     2641 ctgactgaca ggaactggac ctcaagattc gtgggaatac tacctgatgc cgaggccaag
     2701 cgatacgcgg acggccgcat tgatcgctgg tcctggcgcg gtggcgacga attccagcgc
     2761 gaagcgatgc gacgcgcctt catcgagaag gaaggggagc ggctgaagcg gctcatccca
     2821 cctattccat tcgagcttct cgatgttcgg ggggcagatc ttgaatcata caatttcgat
     2881 aagggtggat acatacttgg taatggcctg caatcggaga cgctagtcgg tctgaaactg
     2941 ccattccgtg tgccgtcgtt ctgggcggtt ccgccagaca aggcgcaggc aattctggcc
     3001 caggtgcagg cgaccgcccc tcacaagaac cattcgatct ggcgacaggc ggcattcgcg
     3061 tcgcgactgc gggtcacggg ggtccggtgg gagcgggaca acctcctgat cgacaccgcc
     3121 cttgtgaaga tcgagatgta cgcgggcccg gacctgaaaa caaaacttgg cgacgtgcca
     3181 acgccttgat tggcgcatcc gcgcgcgccc aggacctgaa cacgttcagc catttctcgg
     3241 tcaacgccaa aaccgatcgc ccccatgtac cagttggact tcgccgcatt catgattggc
     3301 gcggttgtcg gcctcgcgct ggccattatt gcgatcctga agatcagaac aaacatggtg
     3361 gaaagcacca tcgagggccg acctgataaa agcgtcggcc gtgtggtcga gatcgtctgg
     3421 acgatttcac tccttcttgc ctttgctcat gtcgttcacc acctgttgct cttcttttgt
     3481 tccttcaacc ccagctacct gttgaggctt cagccagacc cgtctcagcc ctggatattg
     3541 ccggaagctt ggcagatgcc gttttatctg ctggtgctga cctggctgat gagccctctg
     3601 gcagttgcgt ccatcgtggg tcagatctcc tgcggggtgc gactcgcgcc ggaaacaccc
     3661 catcacgcga gcgtggggcg caaggttgcc ttcgcagttc tgaccctgct ggcagtcgtg
     3721 gcgttgctcc cgttgctctt gacggtcccc gtcatctggg agcaggtgct actgccagtc
     3781 atccgagctg tgctcggcat tgcactattg atcatcgtag ccgcggcttt ttctggaaag
     3841 aaaacggacg gtcagccagc atccggtggc aagtcgagtg cggttgccgc aaaagcaccg
     3901 ccgaagaaac aggcatccga caaatgggag cgatgcacga cctgccacgg gcgcggttct
     3961 atcgactgca atgtctgcgg aaatatggga aaaatctctt gcatgagctg tggcggcagt
     4021 ggctcgcagt ggcgttccag cggagccgta acctgcggca catgctccgg ttcaggccgc
     4081 cagggttgcg gttcctgcgg cggttatcga cgttgctcaa gctgtagcgg ccatggcggg
     4141 cgatacgtac gccggtgagc gaggcgctaa ctctggaacg gcatgacctc attgcacgag
     4201 tgagcgttgc gagactacgt ggcgaacaag tcgccctgac gccacgcgaa ctcggcacga
     4261 tgccaggctt tcgacgccgc ctcgcgatcg agccaggcga ggaagggccc gccggcggcg
     4321 gcgagatcgg cctcgtcgag gaagtggctg aggtagccgg tcgaggtgat cggcaggggc
     4381 gcgcccctgg gcgagatcac cgtcagctcg atgtgcgacc agcccttgga gatgtaatcg
     4441 cgcgtgtgac ggacgcggca ggaagcgtga cgccagacga gcttgtgcgt ggcggtcttg
     4501 ggtgccgcct tggcgcgcgt acgacgcgcc ggcttcgacg acgatttgcg agcggtgcga
     4561 accatggcgg tcactccttc ggctggagtg acttgaggaa ggccacggcc tgggaggcct
     4621 tggcggcggc cgtgaagatc gccttcggat cgcgcttcag gagttcgagc cagtgggcga
     4681 tgtactgggc gtggtcggga cgggtgtcct gcgtgacgcc aagctcggcg cagagcaggg
     4741 cggagccgat ttccgcgacc agttcctcgg ccgcgacctc gcatttgtcg aagcgcctgg
     4801 agagatcgcg gtcgcaccgg tgcttcggag ccgtccagtg ggtcagctcg tggaggacca
     4861 cggcgtacca gctctcgtcg cgggtcatgg tctcggtgcc gcagaagagc gcctcgtccg
     4921 gcatggtgat gaggtcagcc gacggccggt agaaagcgcg ctcgccgcca tgctcgatgc
     4981 gggcgccggt ggcgctgatg aagcggtcag cggattccag acgggcgatg ggtccgaggc
     5041 gctcgggtgc ggcgggagcg gtgaagccgt cgacgtcggc ggcgttgaag acataggagg
     5101 cccgggcgac acggcgcttg ccgtcgtcct cggcatcgtc gggatcgggc tcggcgtcgt
     5161 actccttata gaaaatgacc agctcggcct tggcgccctt gcggacctgg catcccttct
     5221 cggcccactg gcggtaggtg gcccagacgg ggacggtgag gccgcgcatc tcggcggcgg
     5281 cccagagcgc gacgacgttg atgccgttgt agagcctgga ggtgtgcgcg ttctcgggga
     5341 gccagagcgg ggcaccggag cggcgccagg gcatctgcgg atggccgggg tccttctcga
     5401 tggcggcgat gatcctgccc gtgatctcgg cgtggaggtc gcggcgcggc ttgagggatg
     5461 gcgcagtcat ggaagacggg tcctttcgct gatgaagccg ccccgatggc ggtttcgatg
     5521 ccgggcgaaa ggcgacggcg gttgagcggg cgcggaaccg gagcgcggaa cgcgcgaggc
     5581 ggcccggcgg agccgaaggc cgagggcttg cccttgaagc gaccgcgcgc cggcgcacgc
     5641 tctggcatca tgaaaaaacc gcctcgggcg gctcctgcga acaggccgcc cccgacggcg
     5701 accatgagct tcagcgccgc gaattctcac ggacagggga gggatgatcg cctgcggcat
     5761 cgcttccggg ccctgcacag aagccgggcg cgatcgggaa cacgggctca accggaagag
     5821 ctgccggcgc tcaacggcat ggtaagatgg cacgcggggc gacgaggcct gggcgcaccg
     5881 gcaaccgcgg gctcggcgaa gccgtcatcc gggatgcggg gtagggccgc aggcgtcagg
     5941 tgcggagcgg gtcgatggat atggaaatct gggacgcccg gatcgccgat tgggtgagag
     6001 ggccgtcgga cgacctcacc gatctcttcg agcggctggt gatcgatgtc gaattgagcg
     6061 aagacgccga cgacgaggac ttcgacccca tgtcgaaggc cgagatgcgc attgaggacg
     6121 ccatccacgc cttcgagtcg acgccgctga atctccggcg gatacggagc tggctacggg
     6181 gcagggtctc tgccgcggct catgaacagc ttctcaagag cgacttccgg gcgctgatga
     6241 gcgaggagga gtacgcgagt gtgatggcat cggcgcggaa gaatttctgc ccgaccatca
     6301 cgaggcagtg gcttggagag actaagccgg aatcattgcg cgccacgacc gcaccgaagg
     6361 cgaaataagg caggcgcaaa tggccgaagt gcaatctcaa gagccaggag gaggggacag
     6421 atgtcagaag cgatgacaag acggacgctg attgcaggat caacggcaat cggagccgcg
     6481 ctcacgtgcg gcatcgagcc gacgaacgag gtcgcgcggg tcctggcggc gctcccggtc
     6541 gtcactggac gtatcaagtg gttcgacgcc tcgaaaggct atggcttcat cgtgccggat
     6601 aaggcgggag cggacatcct gctccacgtc gtgtgcgtgc gggcctcggg tttcgagaac
     6661 gtgcgagagg gctcccgtgt gagagtgaag gtgattgaac ggccaaaggg tcggcaggcg
     6721 gctcagatcc tcgccatgga tacgagcacg gcggtggcga atccggaaat tcgcacacat
     6781 cggattatca gcgcgtagca ggtgacagtc catcgctgat tattgcacca tcctttagcc
     6841 agcgccggta acgccactca aaagtcaacg cgggtatgtg gcggcccagc cgcgccagca
     6901 gaccatgccg gtccacccac agggcatagg tcacatagtc cttgtccgat gccagtagac
     6961 gttcttccac ccatgaacgg gcgaagtaaa ccgagcaggc catgacaaac aggacggtca
     7021 gccgcacaca ctcaacccag ttctctcccg cggttaccgg catccagatg aaaaaccacc
     7081 cgatgcactt tgacaaatag atcggatgac gggtatatcg aaacggaccg gtggttatga
     7141 tgccgcgatg actgaggttg gaggcgcgaa tccccatctg tgcctcgccc cagtaatgga
     7201 caagctcaaa cagaagtatg cagaagccga caacgcagag aaccagcccg tttgccgaaa
     7261 actggctctg ccagttttca gcccggtagt aaaagaacgc tccattgacc agaaatggcg
     7321 cataggacca caatgtaacc acccatgcag acaacgtcct gtcgaccagt cgcacttcat
     7381 ttgaaaacag gcggctggca aacaggtatc ctggtatgat agccgtgacg atcataccgt
     7441 agatgaacat cagcagatcg gcgtgcaacg ccatgacggg tcggtcgaaa taatgttcgg
     7501 gttggatgcg aaaaccgctc atgacccgtg ccgattcaga aaaattcagt ggcagaaaga
     7561 aaacgcggac aagccatgca agggcctcct gcgataagag ctgcgtgtcg atctcgttcc
     7621 atctaccggt cagcagatat ccaagctggc aggatttggc agacgcaggc cccgcgcgcc
     7681 atgtcgtata ccacatgaca aaaaggcaga tgaaaacggc gccgcacgcc actggtacgg
     7741 cgacttcgtg caggtccgca aagctgggac ggcggaaata ggtgatgagc caccaggtaa
     7801 acagcatggc cccgatgcca agacagaccc ctagccatgg aataagcgtc atgcgcaggg
     7861 cttctgccac ggaactcttg acacggggaa cggcgcgcca cggcgctctg gacagttcga
     7921 gtgccacggt gacaacgaca accgacccgc agagcgccac gacaaaccat gcatcgctga
     7981 tgggcaggaa acgcagggag aagaagcccg caaagagggc gagggttacc cagaccggat
     8041 cactccagtg cccgaccgat ctgggaggtc cctggcaatc cactctcgtc gggagtctct
     8101 tggccaaacc tatcacaaat agaagagttg tttacggagc gccgccgtca ccactgctcg
     8161 aggacgtgcc gcagtactga atacagtcat tgtctttgtc tattgttgcc agaaaagtgc
     8221 tggatccatt attgcattga tacatgctgt ccgcgtctcc actgcacgca cagccagtac
     8281 tatcgacaaa atatacctgc atgtgggaat agccaccgca tgatgcgccc ccaccgccag
     8341 gcgtggccca gacaccgtca ccgcggagat aggtcgaact gcttggcgtt ccgctggcac
     8401 tgatccgcgc tgtgggaact gtcaacgccg gagcgatttt cctgcggcgg ccggagtaat
     8461 tttccctcac ggttgaacgc atgaaggccc ccgcgcgcgg gggccttcat gcgtttgcgc
     8521 gtcacttttg cgatggctcg tccgacttgg ccttggaccg gcggcgcttg gagtgggcga
     8581 ggcgatagct ctcgccgttc atctccagga tgtggacatg gtgggtcagg cggtcgagaa
     8641 gggcgcccgt gagccgttcg gagccgaaca cgctcgtcca ttcctcgaac ggcagattgc
     8701 tcgtgacgat ggtcgaggcg cgctcgtagc gctgcgagaa cacctcgaac agcagctcgg
     8761 cgccggtctg cgatagtggc acgtagccga gctcgtcgat gatcagcagc ttgtaggcgg
     8821 cgagctgacg ctggatcctc aggagccgct tctcgtcgcg ggcttcgagc atctcgtgca
     8881 cgagggccgc agcggccgtg aagccgactg acagcccgcg ctggcaagcg gcaaggccga
     8941 gcccgatcgc gacatggctc ttgccggtgc cggagttgcc gagcgcgatg atattgtccc
     9001 gccgctcgac atattcggat cgggcgagct ccatgacgag cgccttgttg agcgacggca
     9061 acgcctcgaa gtcgaagctg tccagcgtct tcacggccgg gaacttcgca gccttgatgc
     9121 ggcggtcgac catgcgcctg tccctgtcga tcagttcctg ttcggccagc cgcagcagat
     9181 agcgtggata gtcgacgcct tcggccgcgc acaggcgcgc ctgtcggtcg tactcgcgca
     9241 ggaacgtcgg cagcttgagg gctttgagat gatgggcgag caagacctgg ggcgtatcgc
     9301 tcatggcgcc cctccactct cgcttacgcc ctcggatgcc cactcgcagc cgggctcgca
     9361 tccggcgcgg agaagcccca tgtaatcgcg cgccgacgtg gtcctgaccg tcgccctcgg
     9421 cagatacgga tagatcgaca gatcgaggcg tgggggccgc cgctcgatcc ggcagagcag
     9481 gagatgcttg atggcatcga acccgacggc cccgcgatcg atgccatcgc gcacggcggc
     9541 ggccacatcg tcgatgcgga acgtctccat cagccgcagc acctgcacga actcgcgctt
     9601 gcccggtttg cccatccgcg cctccagcaa acggcgcagg gtcgcgaact cctctggcag
     9661 ctgccagttg gcgagcggtg cggcctggtc gagcgcattg atcttcttct cgatcaacgc
     9721 gaggtagtgc agcggatcga agatgaagtc ctcgcgctca taggagcgtg gatgacgcgc
     9781 gatcacctcc gtaccgcagg cgatgaccac ctcgtgcacg tagccacgca cgagcacttg
     9841 cgcgtggccg aactcggtcg ggaccgagta gtcattgccc cgatagcgca cgagcgacag
     9901 cgaggagacc gtcgtcggaa ccttctcgca ggcgtcgtac ggtgggggca acggcttgcg
     9961 cagggcatcg agatcgcgtg ccaatcgttc gccgatcgtc tcggaatgcc cccgcagccg
    10021 ttcagagagt cgcttgcggc agcgccctgc cagatcggcg ttgaacgtct cgaagcagtc
    10081 gaacgccggt accggcacca tgaagttgcg gcgcgcgaag ccgacgagcc cttcgacctt
    10141 ccccttgtcg ttgcccttgc ccggccgccc gaaccggtcc tggaacaagt agtgcgattg
    10201 cagctccgag aatgtcctcg tgcgctggcg cgtgccatcg cccaggatgc gggcaacggc
    10261 gatcctggtg ttgtcgtaga ggattgatcg aggcacgccc ccgaagaact cgaaggcctt
    10321 cacatggccg tcgcaaaagg cctccgtcgt ctctgctgga taggcgatca cgaagcaggc
    10381 gtccgagtgc ggcagatcga acgcgaagaa gtggaccttg cgcttggttc ccccaatgac
    10441 cgccagcgcc tccccgaagt cggcctgggc atgaccgggc ggatgagcga gcggcacaaa
    10501 catctcccgt gaccgctgcc gcgagcccgc cacgtaatcc ttcacgatcg taaggccgcc
    10561 gctatagcca tgctcgtccc ggagccgttc gaagatgcgc ttggacgtgt gctgctgctt
    10621 cgtcggccgg tccttatcgt cggccaggat cttgtcgatg aacccgatga agggatcgag
    10681 cttgggcttc accgccggct tcgtgcgccg gtatcccggc ggtaccgaga acctcatcat
    10741 cttggcgacc gtcttcggat cgatcccgta tcgacgagcc gcctcccgat ggctcaaacc
    10801 ttcgatctga accgcgtagc gaacctgtgc gtagagctcc accctcttca tcccccgccc
    10861 tctgccggac gcggcacagg gctcgtcagt gccggaattt tactccggcg caaccgaccg
    10921 actcggctgc ttcactgagg gatttttgct ccggcgctta cacccgtttt gcttcatcga
    10981 gctttttctt tagtgccttg ctcaattccg gagcgacgag tactgtcaga ggatcggtag
    11041 cgcttaggac tgaccgcttc aagtggtcga cgcggtactg cctactgaag gttctgccga
    11101 agcatccgga ttttgtgtct acgatgtgct cgatcaaatc atgcaagcag agcccatctc
    11161 gggcataggc agattgccat tctacaagca ggcttgttac tttcttattt ttgtgacgca
    11221 ttacgttagc gagcgttcta attgtcgtcg tggctaatag tcgttcatat aatacgaaat
    11281 tgaaattacc atattagtga aattacttgc aagtcttgtt atcagttgag ctctgccgat
    11341 cttgagtatt cgatggggag cgggatattt tctataatgt gataatgtta cttatgggaa
    11401 ataatgccgg ttatcgaaat cagctgttgc gcaaatctcc gatgaattcc gagcccggat
    11461 tgaggccgga gatgagcagt cggtcgcgtg ccctggtgca ggcaacgtag aacagctggc
    11521 gctcggtggc gtagacgtcg tcaagctcga tctcgtccgc gacggccgcg atgcgatcct
    11581 gaagcggcaa aatagcctcg tcgcaggcca tgacggccac ggccttgaac tccaggccct
    11641 tggccaaatg catgatgccg atgcgaatgg cgctgacggg accttcttta cgcggcgtga
    11701 gctcagcgac cgccagtcca agccgctcgg cggcggcgcg cgctcgcgac aatacttcgc
    11761 gggttctgac gaacagcccg atctcctcgg gcttggtgcc gtcgcgcatg gcggcttcga
    11821 tgaagctcgc cacggcggag atttcaccct cctcgctgtc gtgtagagat atcgtaggaa
    11881 ccggaccatt gaagacggag accgtcccgc ggcgatcctc ctgatgcccg tcgacatccg
    11941 cgacaggacc tgagaacaac cgatcagcgg cctcacgaat ttggtgtgat gtccggtagt
    12001 tgactttgag cgtcagggag cggccttgca cgtctatgcc gaggcctttc caggagaagg
    12061 gttgctggaa gatgcgctga ccgagatcac cggcgaagaa caaagcgtcc gggccggacg
    12121 gcacgatgga cttcaggaaa cgcaactcgg ggacgccaag gtcctgcgct tcgtcgacga
    12181 ccacgtgcgt gaacggcttg tccgatctcg cgccatagtg gtcggtgagc ttctggaaca
    12241 tgctggccgt ggtttcgaac ccgcgtgcgg cgagatcatt cagaacactc tggaaaatcg
    12301 gccagagccg ttcgcgctgt ttggcgccca tgcggttctt gcggccgagg cggggcacct
    12361 cggcataggc ctcggcactc ttgatctgcc aggcatcgac gacgtaggcc cactccgagg
    12421 cgaggaagcg ctccgagagc gttgatccgg cctgtgcctg agcggccttg acgagttggc
    12481 cacggatcag ctcctcgctc gcgatcgccg gcttgccacc aaacacgagc tgaaacagct
    12541 cgcgcgctat gccgtcgaat gaggctactg tgatccgcgg caccacttcg gatgtctctc
    12601 cggccagaat ctggagtttg cgctcgagcg cgaatgccaa cggctcggag aatgtcgtca
    12661 atagcacccg agagcaagaa tcgcgcgcca gtcgccgtgc gcgatggatg gcgacgactg
    12721 tcttgccagt gccggccgac cccgcgaccc gcgcggggcc ggcgaacgac ctctcgacca
    12781 gatcgcgttg cgaggggtgg agaaagacga tccatcggtc ccagggatag gagagcgcct
    12841 gttccagttc ctcgacgttc tcgacgacgc gaaaccgccg tagggcatcg gggtgcgcga
    12901 acgggtcggc cactacgatc ggcgccggct tcttcagcga gcctgtggcg acgtactcca
    12961 gtagagcttc ggatgcctct ccgggcaggt gtgttgccag ctccaggaat ccatcctcgg
    13021 tcgcgctgat gatgtcgccg atccagtctt ccggcacccc caccccgaga agatcgtcgc
    13081 gcgtcagcga gacgaagggc gtgatcggcg ccacgggcgc ccggcccttg cctttgggca
    13141 aaggcagttc acccggacgc ggcaggtcga gcggctcctg ctggggccgg acatagatgg
    13201 gcaattcctc gacccgctcg cggacctcga cgatctgaat ggcgccggtc ttggggtgcg
    13261 cctcgatgcg tcggcgctcg gcccacttgt aggcatcgtc gtggtgatcg acgtaggcga
    13321 gcagaaagct ctgcgctgtc ttatggacga tgatacggat gtcgcggttg acgcgcacgg
    13381 accagaagtt cgggtccttc gaggtatcga tccgatgaag ctgaagccct ggattggtcg
    13441 gattggtctg cagatcgaac gccgacgttt tgaccgcctt ctgctcgtcg cgcgtgagcc
    13501 tgccgagggc gaacgtgaag gtatcggcga tgcgaaattc catacgtgtg cctccctcag
    13561 ctccttgcct cgcctcggag tatgaggcca agcagaaacc caagtgcgaa tgcgcccgcg
    13621 acccatgccc agaagtacgt gcctgctact ccgaagaaat cgacaaacca tttcgtgccg
    13681 cttactgcgt acgacagtcc agcaattgcc gggagaagtg cagcaatgac caagccgacc
    13741 gccgcttgat tgggcacatg cttctcgtat gcggaacccc gcagaagcgt gagaacctgg
    13801 ccaaggtgct gtggccggaa tgcgtagatg gcaagcacga agccaagtat agctccgcca
    13861 cataccagca cagccacaag gccgaagaag aatatcaaga tcatgattta tacctgaaac
    13921 accttcatca cctcgtcgcc gaagtgattg atgaccttga tggcgatgcg gccagtcgac
    13981 gggcgcggga acggccgcga ggtgtcgcgg tagagcgtct cccaggcatc gggattgatc
    14041 tcggcgcgga gcgcggtctt gagcgacttg tagggatcgt tggcgccgag gaagtaggcg
    14101 tgacggacga agaagctctc ctcgttgtag tccgtgtcga tgaaccaggc ggcgatgccc
    14161 ttgacgtcgt tcgaacggat ctggccggtg ttcggatcga acacgtcgat gccgttgacc
    14221 ttgacgacaa tggtctcgtc gccctgatcg agtatgtcga tgtccggctc gccgaagatg
    14281 acgaagaggt tgcccttgcc cgcgttcttc agctcgtcgg cc
//
LOCUS       DAORNH010000091        14103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3254_length_14103_cov_2.990390, whole genome shotgun sequence.
ACCESSION   DAORNH010000091 DAORNH010000000
VERSION     DAORNH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_14103_cov_2.990390"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK264_11005"
     CDS             <1..188
                     /locus_tag="PK264_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD76451.1"
                     /translation="GAGAESRRQIGWVIVGGMSIGTLLTLFVVPIVYSLIGRRHVLAE
                     EAAPMVRGKEITAAPAE"
     gene            complement(194..1438)
                     /locus_tag="PK264_11010"
     CDS             complement(194..1438)
                     /locus_tag="PK264_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD76452.1"
                     /translation="MRVLSRGRAEAVVAAFDLTRLNPAFYADPYPVYHALRELRPVHR
                     MASGSVLLTRHADLEAIYKDTTLYSSDKRIEFRPKFGDGPLFEHHTTSLVFNDPPLHT
                     RVRRIIAGALSPRAVTEMEGPVTALVDGLLDQMAGRGEVDLIEDFAAAIPVEVIGNLL
                     DVPREERGPLRDWSLAILGALEPVLTSEQLARGNRAVTEFLAYLRILVATRRAKPGDP
                     EKDVLTRLIGGEGDGAQLTEMELMQNCIFILNAGHETTTNLIGNGLVLLTQWPEERRR
                     LIGDPSLIKSAVEEMLRFESSNQLGNRMTTAPVTIGGEALAAGTSITLGIGAANRDPA
                     VFSAPDRFDITRRANRHLAFASGPHQCVGLAVARLEGRIAIERFLGRFPRYALAGRPR
                     RGGRARFRGFLSVPARLEFARH"
     gene            1675..1872
                     /locus_tag="PK264_11015"
                     /pseudo
     CDS             1675..1872
                     /locus_tag="PK264_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085938460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1947..3095)
                     /gene="proB"
                     /locus_tag="PK264_11020"
     CDS             complement(1947..3095)
                     /gene="proB"
                     /locus_tag="PK264_11020"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRD76453.1"
                     /translation="MTASIVPRTPRPEWAAARRIVIKIGSALLVDRGNGRLRADWLNS
                     IADDIAALAAEGREVIVVSSGAIALGRHLIKLPKGPLALEQSQAAAAVGQISLARAYQ
                     ETFAARGLTTAQVLLTLGDTEERRRYLNARRTIDTLLAHKAIPVVNENDTVATSEIRY
                     GDNDRLSARVASMMSTDCLVLLSDIDGLYTAPPGKGVDARRLDVVRAITAEIEAMAGD
                     AGSELSRGGMVTKIEAGKIALAAGSHMIITSGKVMHPLRAIADGAPCTWFLAPSDPVA
                     ARKRWIAGQLEPKGTVEIDQGAERALAQGKSLLPAGVKRVDGAFDRGDAVIIRAPDGR
                     EVGRGLIAYPIDEARRIVGRKSSEIADILGHEGRAELIHRDDMALSRG"
     gene            complement(3202..5295)
                     /locus_tag="PK264_11025"
     CDS             complement(3202..5295)
                     /locus_tag="PK264_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD76454.1"
                     /translation="MNDAPSPLRTVTSACPHDCPSTCALEVEMLAPDRIGRVRGAKGN
                     SYTAGVICGKVARYAERIHHPDRLLYPLKRKGPKGGSAFERIGWDDALDIVGEQFLAA
                     ERLHGSEAVWPYWFAGTMGLVMRDGIERLRNVKRYSRQLATICTTPAWSGFVAGTGKL
                     AGPDPREMAKSDLVVIWGTNPVNTQVNVMTHAVRARKERGAKIVAIDIYQNGTMEQAD
                     VALCLRPGTDGALACAVMHILFRDGHANWPYLEKYTDCPRELEAHLKSRTPEWASAIT
                     GLSVSEIETFARLVGTTPRTYLRLGYGFSRQRNGAHNMHAASCIASVTGAWLHEGGGA
                     FHNNGAIFHWNKTLIEGTDVADPAIRMLDMSRIGPVLTGDRSDLGEGPPVTAMLIQNM
                     NPAQVAPEQLKVRAGLMRDDLFVVVQEQFMTATARLADIVLPATMFLEHDDVYQGGGH
                     QHILLGPKVMEPPGECRSNHEALVGIARRVGAHHPGFEMSPRELIDWTLRQSGWGTLA
                     ELEKVKWIDCQPEFATAHYTSGFAYPDGRFKFKPDWPAVPAARKWDWGIAQGMPRFPD
                     HWDVIEKADARHPFRLATSPSRGYLNSSFNEMPSVRKREGKPRAKMHPEDMRALGIAD
                     GERIEMGNERGTIGLTAEVFAGLQRGVVVVEGIHPNEDFEGGRGINTLTSADRIAPFG
                     GAAFHDNHVWIRRAA"
     gene            5603..5679
                     /locus_tag="PK264_11030"
     tRNA            5603..5679
                     /locus_tag="PK264_11030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5637..5639,aa:Pro,seq:cgg)
     gene            complement(5802..6896)
                     /gene="ugpC"
                     /locus_tag="PK264_11035"
     CDS             complement(5802..6896)
                     /gene="ugpC"
                     /locus_tag="PK264_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRD76455.1"
                     /translation="MSNVSIRKIHKRYDDGFHAVKGIDLEIRDKEFVVLVGPSGCGKT
                     TTLRMVAGLESVTEGQVLIGPQVVNDLPPMDRDIAMVFQNYALYPHMSVYDNMAFGLK
                     MRKFDKAEIRKRVANAAEILGIKDHLERKPRQLSGGQRQRVALGRAIVRNPQVFLFDE
                     PLSNLDAKLRVQMRIEIKKLHQRLATTSMYVTHDQVEAMTLGDRVVVMNHGVIQQVGE
                     PLELYNNPANKYVAGFIGSPPMNFIEVTVANEGGALYATSPTVRIRVPDDIAARMGPH
                     VGKRAFLGIRPEDLRMANGAEPDGTTLDAVVEVAEQLGSEIILDLKAGGGAMVASVEP
                     TVRVKPQEKIRLALNPVGLRFFDGTTEARI"
     gene            complement(6935..7294)
                     /locus_tag="PK264_11040"
     CDS             complement(6935..7294)
                     /locus_tag="PK264_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRD76456.1"
                     /translation="MAGVVNFRDLQIWQKSMDQCVATYSVSKLLPKDELCGLASQMRR
                     SSVSVPSNIAEGFARQGATFAQHLKIAQGSLKELETQLLICQRLDLLSEGKFVPLHQL
                     CDEIGRMVRSFSRTIAT"
     gene            complement(7334..8185)
                     /locus_tag="PK264_11045"
     CDS             complement(7334..8185)
                     /locus_tag="PK264_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRD76457.1"
                     /translation="MIEGAAWRRWVFFRIPLLVFLLFLLFPFYWMVITTVRPDNELYI
                     PWMRPQYKPLWTWNPTLAHLKYLHGETTFWLWMWNTTFIAVVATAVSLVCGVLAGYAL
                     SRLKFSMAGLLGTGIFVTYLVPQTLLFIPLAEIIRAYNIGDTPWSLILTYPTFLIPFV
                     TWLMMGYFKAIPKELEECARIDGASRWQAMLYIIIPVAIPGILSAMIFAFTLSWNEFI
                     YALVFLSSPEQKTVPVGVISELVRGDVFFWGPLMAGALLGSIPVAIAYSFFVEHYVSG
                     LTGSVKG"
     gene            complement(8185..9114)
                     /locus_tag="PK264_11050"
     CDS             complement(8185..9114)
                     /locus_tag="PK264_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRD76458.1"
                     /translation="MAVPAAPSGDVIVAKPKWRPLESEGNLALLLLLPTLVLLGLFIA
                     YPFLRGIMLAVTDTKVGEPGKWVGFENFGRLLADPIFHAVVKNTFLYTFVTTIFKLAL
                     GLWLAILLNRNFKFKAFTRAFILLPFIVPTVLACFAWKWMFDPTFSVVNWFLWKAGVI
                     TNSINWLGDGDLAMLSIIIVNVWRGVPFFAISLMAGLQTISPDLQEAAAIDGAKPWQR
                     FIYITWPLLLPVTMVVVLFSVIQTFADFQIVYVLTGGGPANATHLFATYAYQLGIGTG
                     LLSQGAAVSLAMFPLLFLIVIFQLMYIRRVETR"
     gene            complement(9257..>9987)
                     /locus_tag="PK264_11055"
     CDS             complement(9257..>9987)
                     /locus_tag="PK264_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76459.1"
                     /translation="GQSFGDPPTFAYPLLWSYGGAEIDDKGKVVINSKETVDAVKWMA
                     SAWKESFDEGGLAWDDASNNRAFLAGTISATLNGASIYIETLRKPDQYKTAEAKPLKD
                     DVFHGPLPAGPKGQFGMHTFTSHVMPSYSKNQKAAKELLRFFHKKENYDQWFSTGMGF
                     YTPATTGWESHPIWTKDPIMAPFAVAGKLGATPGHPGEPNAKAAEVLTKYLVGNMFAA
                     AIKGQAAEEAVKACEAQLKTIYGA"
     assembly_gap    9988..10082
                     /estimated_length=95
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10083..10549)
                     /locus_tag="PK264_11060"
     CDS             complement(<10083..10549)
                     /locus_tag="PK264_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76460.1"
                     /translation="MSRTTRRTFLKKSAAAAGGMAGILATGRAPAFAQTAEVHWLRWN
                     DFIPACDQLLRTKLLPEAEKALGIKVKYETVNGNDLQPRITSSIQSGAGPDLIMLFNN
                     NPHLYAKSLVDLSDVADEVGKAQSGYYKVSSANCGTGGKFFSMPAAIIGAMNI"
     gene            10814..11383
                     /gene="efp"
                     /locus_tag="PK264_11065"
     CDS             10814..11383
                     /gene="efp"
                     /locus_tag="PK264_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRD76461.1"
                     /translation="MAKVIASSIRKGNVLEIDGKLCAVMKAENIHPGKGTPVTHLEMR
                     RISDGIKVVERYRTTEQVERVYLEEAQYNYLFEDDNGFTFMHPENFEQVQVNKDIIGD
                     QSVYLQENMVCTLQIYNGVPVSCELPPRVTLEIIEADPVVKGQTAASSYKPAILSNGV
                     RVMVPPHISSGTRIVVSTADGTYVERSKE"
     gene            complement(11393..13588)
                     /locus_tag="PK264_11070"
     CDS             complement(11393..13588)
                     /locus_tag="PK264_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD76462.1"
                     /translation="MPSEHRDTVVPEPDQPRAGRASAFARAGQTVEQLGLTPHGLVAI
                     AGYAMLAASVTRLAGPIGIAHVAIILSGSLLALALALAARARTVEVERPAAPLPDSET
                     VRDAEWSLRESEARLRALLDHHPDAILRRDPSGNLTYANRTAERLLGEAGQLPETVRD
                     GGDCCRPIGPPDEPRWLVWSRKELPDVDGLPGGIEIIGRDITETRAAEIALARRRDEA
                     QSASEAKSRFLASMSHEIRTPMNGILGMATLLRDTSLSADQATYVDAVDRSAKALLKL
                     VNGILDLSKVEAGRMELLAEPVSLSDCVGGVIELLAPNAAEKGLELAWTMSADLPATI
                     PTDEMRVRQILLNLIGNAIKFTDAGGVAVRIERRGEGIAVRIADTGPGIAPAVLASLF
                     AEFVQADTAIQRRIDGSGLGLAISRSLARALGGEIEVVSKLGEGSEFTMLLPVPGMPA
                     ASALPVKALASEIIVVELSGTIEQAMTTATIARAGGRVIDVRVADHVTMSPIKAVVLD
                     TGRGEEKAAAILKRVREVSLGLRPRGIIVIDASERDRLPHLRTLGFDAYLVRPVRPQS
                     LVTQIVVDAAPLPIGPVPDPSGPVRPDCRLPDCRVLLVEDNDINALLTCRLLERLGVV
                     VIREREGLGAVTAAEQHFDLVLLDIHMPGIDGHETRARLASLYRSSGRIAPPIIAVTA
                     GAFPEDRKHCLDSGFDDYLAKPFSADEVAAMLAKWGNGMRSRRAGGIVC"
     gene            13728..>14103
                     /locus_tag="PK264_11075"
     CDS             13728..>14103
                     /locus_tag="PK264_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD76463.1"
                     /translation="MGPLKGFKIIEMAGIGPAPFCGMVLADMGAEVIRVDRREGADLG
                     LPGREPKYDVLHRGRQSIAVDVRTEGGREVVKALVAKADALIEGFRPGVMERLGLGPD
                     DLAKVNPKLVFGRMTGFGQTGPM"
BASE COUNT         2432 a         4454 c         4734 g         2388 t
ORIGIN      
        1 ccggcgcggg cgcggagagc cgccgccaga tcggctgggt gatcgttggc ggaatgagta
       61 tcggcacgtt gctcacgctg ttcgtggtac cgatcgtgta cagcctgatc gggcgccgtc
      121 acgtcctggc ggaggaggcg gcaccgatgg tacgcggcaa ggagatcact gcagcaccgg
      181 cggagtaacg gcgctaatgc cgcgcgaact cgaggcgtgc aggcaccgag agaaagccgc
      241 gaaaccgcgc ccgtccgccg cgccgtggcc gaccggcgag cgcgtagcgt gggaagcggc
      301 cgaggaaccg ctctatggcg atccgccctt caagccgcgc gaccgcgagg ccgacgcact
      361 ggtgcggccc tgaggcgaag gcgagatgcc ggttcgcccg gcgcgtgatg tcgaagcggt
      421 cgggggctga aaacactgcc ggatcgcggt tcgccgcgcc gatgccgagc gtgatcgacg
      481 tgcccgcggc gagcgcctcg ccgccgatcg tgaccggcgc cgtggtcatg cggttgccga
      541 gctggttcga gctctcgaag cgcagcatct cctcgaccgc actcttgatg agcgatggat
      601 cgccgatgag gcgccggcgc tcctccggcc actgcgtgag cagcacgagg ccgttgccga
      661 tgaggttggt cgtcgtctcg tggccggcgt tgagaatgaa gatgcagttc tgcatcagct
      721 ccatttcggt gagttgcgcg ccgtcgccct cgccgccgat gaggcgcgtc agcacgtcct
      781 tctcggggtc gccgggcttt gcccgtcgcg tggcgacgag gatgcggaga taggcgagga
      841 actcggtcac ggcgcgattg ccgcgcgcga gctgctccga ggtcaacacc ggctctagcg
      901 cgccgaggat ggcgagcgac cagtcgcgga gcggtccgcg ttcctcccgc ggcacatcga
      961 ggagattgcc gatcacctcg acggggatcg ccgccgcgaa gtcctcgatg agatcgacct
     1021 cgccgcgccc ggccatctga tcgagaagcc cgtcgaccag cgccgtgacc ggcccttcca
     1081 tctcggtgac cgcccgcggc gagagcgcgc ccgcgatgat ccggcgcaca cgcgtgtgca
     1141 gcggcggatc gttgaagacg agcgaggtgg tatggtgctc aaagagcggg ccgtcgccga
     1201 acttcgggcg gaactcgatc cgcttgtcag aggagtagag cgtcgtatcc ttgtagatcg
     1261 cctcgagatc ggcgtggcgc gtgaggagca cgctccccga cgccatgcgg tgcaccggcc
     1321 ggagctcgcg gagcgcgtga tagacgggat agggatcggc gtagaaggcc ggattgagcc
     1381 gtgtgaggtc gaaggccgca accacggcct cggcgcgccc acgcgagagg acgcgcatcc
     1441 cgcggtgatc catcggcagg ctgccggggt aggcatcggt cgacggctga tcggacatga
     1501 cggctccgcg aaagcgcagg tggccggaca gcttataccg aaacgctgga tgataaggcg
     1561 ttcgggcacg atcacggccg ccgaatcgtc atgttccgcc agcgtcgaac cccatttccg
     1621 tcagtgaacc gccccggaat tgccggaggc tccaacgctt gagagactgg agccatgaca
     1681 cgcggatcaa ggtcttcgaa gttcgcgcag gaagtgcgca atcgcgcggt gcggatggtg
     1741 gccgaccacc gtgacgacca cgcctccgag tggccggcga tctcgtcgat cgacggcaaa
     1801 ttcgactgca agcccgagag gctgcggctg tgggggcggc agactcagcg cgaccagtac
     1861 aagcggcctg gcaccgcccc gcgaataaga actggtccca gccctcgctc ctcggtcgac
     1921 gcgcttatcg cgccttagac gccacactag ccgcggctca gggccatgtc gtcgcgatgg
     1981 atgagttcgg cgcgaccctc gtgcccgaga atgtcggcga tctcgctgct tttccgaccg
     2041 acgatgcggc gcgcctcgtc gatcggatac gcgatcaagc cacggccgac ctcgcggccg
     2101 tcgggcgcgc ggatgatgac ggcatcacca cgatcgaaag cgccgtcgac gcgcttcacg
     2161 cctgcgggca gcaggctctt tccctgcgcg agcgcgcgct cggcgccctg gtcgatctcg
     2221 accgtaccct tcggctcgag ctggccggcg atccagcgct tcctcgccgc cacgggatcg
     2281 gacggtgcga ggaaccaggt gcacggcgcg ccgtcggcga tcgcccgcag tgggtgcatc
     2341 accttgcccg aagtgatgat catgtggctg ccggccgcga gcgcgatctt gccggcctcg
     2401 atcttcgtca ccatgccgcc gcgcgagagc tcagagcccg cgtcccccgc catcgcctcg
     2461 atctcggccg tgatcgcgcg cacgacatcg aggcggcgcg catcgacacc cttccccggc
     2521 ggcgccgtgt aaagaccgtc gatatccgag agcagcacga ggcagtcggt gctcatcatg
     2581 ctggccacgc gcgccgaaag ccgatcattg tcgccgtagc ggatctccga ggtcgcgacc
     2641 gtatcgttct cgttcacgac ggggatcgcc ttgtgggcga gcagcgtgtc gatcgtgcgc
     2701 cgtgcattga ggtagcgccg ccgctcctcc gtatcgccga gggtcagcag cacctgcgcg
     2761 gtcgtcaggc cgcgcgccgc gaacgtttcc tggtaggcgc gcgcgaggct gatctgcccg
     2821 acggccgccg ccgcctgact ctgctcgagc gcgagcggcc ccttcggcag cttgatcagg
     2881 tggcgcccga gcgcgatcgc gcccgaggac acgacgatca cctcgcggcc ctcggcggcg
     2941 agtgcggcga tgtcgtccgc gatcgaattg agccagtcag cgcggagcct gccgttgccg
     3001 cgatcgacga gcagcgccga gccgatcttg atgacgatgc ggcgcgcggc ggcccactcg
     3061 gggcgcggcg ttctgggcac gatgcttgct gtcatgggat ttgaggtcgc cgtcattaat
     3121 caaaagatgg tgcgggccga tccgcgccgc cgctgatagc aggtcgcctc gggcttgggc
     3181 agtcgcattg agcgaatggc cctacgctgc ccggcgaatc cagacgtgat tgtcgtggaa
     3241 ggccgcgccg ccgaagggtg cgatgcgatc ggcactcgtc agcgtgttga tgccgcggcc
     3301 accttcgaag tcctcgttgg gatgaatgcc ctcgacgacg acgacgccgc gttgcagccc
     3361 cgcgaacacc tcggccgtaa ggccgatcgt gccgcgctcg ttccccatct cgatgcgctc
     3421 tccgtcggcg atgccgagcg cccgcatatc ttccgggtgc atcttggcgc gcggcttgcc
     3481 ctcgcgcttc ctgaccgatg gcatctcgtt gaacgaagag ttgagatagc cgcgcgacgg
     3541 cgatgtcgcg agccggaagg gatggcgcgc gtcggccttc tcgatcacat cccaatgatc
     3601 ggggaaacgc ggcatgccct gcgcgatacc ccagtcccac ttgcgggccg cgggcacggc
     3661 cggccagtcc ggcttgaact tgaagcggcc gtccggatag gcgaagccgg acgtataatg
     3721 cgccgtcgcg aactccggct ggcagtcgat ccatttcact ttctcgagct cggcgagcgt
     3781 gccccagccc gattggcgca gcgtccagtc gatgagttcg cgcgggctca tttcgaagcc
     3841 cggatggtgt gcgccgacgc gcctggcgat gccgaccagg gcctcgtggt tcgagcggca
     3901 ttcgcccggc ggctccatga ctttcggccc gagcagaatg tgctggtggc cgccgccctg
     3961 gtagacgtcg tcgtgctcga gaaacatcgt cgccggcagt acgatgtcgg cgaggcgcgc
     4021 cgtcgccgtc atgaactgct cctggacgac gacgaagaga tcgtcgcgca tgaggccggc
     4081 gcgcaccttc agctgctcgg gcgcgacctg ggccggattc atgttctgga tcagcatcgc
     4141 cgtgacaggt ggcccctcgc cgaggtcgct tcggtcgccg gtgagcaccg ggccgatgcg
     4201 gctcatgtcg agcatgcgga tcgcagggtc ggcgacatcc gtcccctcga tcagcgtctt
     4261 gttccagtgg aagatcgcgc cgttgttgtg gaaggcgccc ccgccctcgt ggagccaggc
     4321 gcccgtcacc gaggcgatgc agctcgcggc atgcatgttg tgggcgccgt tgcgctgacg
     4381 cgagaagccg tagcctagtc gcaaatacgt ccgcggcgtg gtgccgacca gccgtgcgaa
     4441 cgtctcgatc tcgctgactg agagtccggt gatggcgctc gcccactcgg gcgtcctgga
     4501 tttcaagtgc gcctcgagct cgcgcggaca atcggtgtac ttctcgaggt aaggccagtt
     4561 ggcgtgccca tcacggaaca ggatgtgcat cacggcgcag gctagcgcgc cgtcggtgcc
     4621 gggcctgagg cagagcgcga cgtccgcctg ctccatcgtg ccgttctgat agatgtcgat
     4681 cgcgacgatc ttcgcgcccc gctccttcct cgcacgcacc gcatgcgtca tcacattgac
     4741 ctgcgtgttg accgggttcg ttccccagat cacgacgaga tcggactttg ccatctcgcg
     4801 cgggtcgggt cccgcgagct tgcccgtgcc ggcgacgaaa ccggaccagg ccggcgtcgt
     4861 gcagatggtg gcgagctggc gcgagtagcg cttgacgttg cggagccgct cgatgccgtc
     4921 gcgcatgacg aggcccatgg tgcctgcgaa ccaatagggc cagacggcct cgctgccgtg
     4981 caggcgctcg gctgcaagga actgctcgcc gacgatgtcg agggcatcgt cccagccgat
     5041 cctctcgaag gcgctgccgc ccttcgggcc cttgcgcttc agaggataaa gcaggcgatc
     5101 tggatggtgg atgcgctcgg catagcgcgc gaccttcccg cagattacac ccgcagtgta
     5161 gctgttgccc ttggcgcccc gcacccgccc gatgcggtcc ggcgccagca tctcgacttc
     5221 gagtgcgcat gtcgatggac aatcgtgcgg gcaggcgctg gtgacggtgc gcaatggcga
     5281 gggtgcgtcg ttcatggccg gctctcgctc ggttggcgcg ttcaggaaca cgccatcgac
     5341 cttagcggta ccgacgcgaa cgagaaagcc gctccttgcg cggttctcgc cctgcgccac
     5401 ggacacttcg cccggctgca ccggcgacgt agggaaggcg gcgaggaagc acttgctctc
     5461 cgccgcgaac ctcggttata agccggcgtc ccgacaccgc gatctgcggt gactacggcg
     5521 gcttcactca ttgacgtgct ggttcatcca ggacgcgctg gcccggccag aaatcgatgg
     5581 gacgaggccg caatcgactt ggcggagcgt ggcgcagcct ggtagcgcac ttgcttcggg
     5641 agcaaggggt cggaggttcg aatcctctcg ctccgaccat ttctcaactg aaaatgaaat
     5701 atttgatcat gcagctgcaa tcgcgtccgt tgacgcgcac gacaagcggt cgtgatcaag
     5761 gcggaaattg cccgccgcca cgcctgtgat cgcgactgct gtcagatccg cgcttctgtt
     5821 gtgccgtcga agaagcggag accgacggga ttgagggcca ggcggatctt ttcctgcggt
     5881 ttcacgcgta cggtcggctc gacgctcgcg accatggcgc cgccgccggc cttgaggtcc
     5941 aggatgatct cggagccgag ctgctcggcg acctcgacca cggcgtcgag cgtcgtgccg
     6001 tcgggctccg cgccattcgc catgcgcaga tcctcgggcc ggatgccgag gaaggcgcgc
     6061 ttgccgacgt gcggccccat gcgcgcggcg atgtcgtcgg gcacgcggat gcgaaccgtc
     6121 ggcgacgttg catagagggc tccaccctcg ttcgcgaccg tcacctcgat gaagttcatc
     6181 ggcggcgagc cgatgaagcc cgcgacatac ttgttcgccg gattgttgta gagttcgagc
     6241 ggttcgccga cctgctggat gacgccgtgg ttcatgacca cgacgcggtc accgagcgtc
     6301 atcgcctcga cctggtcgtg cgtgacgtac atcgaagtcg tggcgaggcg ctggtgcagc
     6361 ttcttgatct cgatgcgcat ctgcacgcgg agtttcgcgt cgagattcga caagggctcg
     6421 tcgaacagga agacctgtgg gttgcgcacg atggcgcgac cgagagcgac gcgctggcgc
     6481 tggccgcctg aaagctggcg gggcttgcgc tcgaggtgat ccttgatgcc gaggatctcg
     6541 gcagcgttgg cgacgcgctt cctgatctcg gccttgtcga atttccgcat cttcaggcca
     6601 aaggccatgt tgtcgtagac cgacatgtgc gggtagagcg cgtaattctg gaacaccatg
     6661 gcgatgtcgc ggtccatggg cggcagatca ttgacgacct gcgggccgat caggacctgc
     6721 ccctccgtca cgctctcgag accggcgacc atgcgcaagg tcgtcgtctt accgcacccc
     6781 gaggggccga caagcaccac gaattccttg tccctgatct cgaggtcgat gcccttcacg
     6841 gcatggaacc catcgtcgta gcgtttgtgg atcttgcgga tggagacgtt tgacatgtga
     6901 agtccttagt acctggtact tagtacttag ctgactatgt ggcgatggtg cgggagaacg
     6961 atcgaaccat tcggccgatc tcgtcgcaga gttgatgcag aggcacgaac ttaccctcgg
     7021 acaagagatc gagccgctgg cagatgagca gttgggtttc gagttccttc agcgagccct
     7081 gagcgatctt gagatgttgt gcgaaggtcg cgccttgtcg cgcaaaaccc tcggctatgt
     7141 tcgacggaac agacactgac gagcgccgca tctgagatgc gagaccacag agttcgtcct
     7201 tgggcagcag tttcgaaacg ctatacgtcg ctacgcactg gtccatggac ttttgccaga
     7261 tttgcaaatc cctgaagttg accacgcccg ccatctgcct gcccctaagg actaagtact
     7321 aggtaccaag cactcaccct ttgaccgaac ccgtcagccc cgacacatag tgctcgacga
     7381 agaagctgta cgcgatcgcg actgggatgc ttccgagcaa ggcgccggcc atcagcggcc
     7441 cccagaagaa gacgtcgccg cgcacgagct cggagatgac gccgacaggc acggttttct
     7501 gctcgggcga ggagaggaac acgagcgcgt agatgaactc gttccagctc agggtgaagg
     7561 cgaagatcat ggcggagagg atgcccggga tcgccacggg gatgatgatg tagagcatcg
     7621 cctgccagcg cgaggcgccg tcgatgcgcg cacactcctc gagttccttc gggatcgcct
     7681 tgaagtagcc catcatcagc cacgtgacga acgggatgag gaaggtcggg taagtgagga
     7741 tcagcgacca gggcgtatca ccgatgttgt aggcgcggat gatttcggcg agagggatga
     7801 agagcagcgt ctgtggcacg aggtaggtca cgaaaatgcc ggtgccgagg aggcccgcca
     7861 tcgagaactt gagccgcgag agcgcatagc ccgccagaac gccacagacg agcgaaactg
     7921 cggtcgcaac gaccgcgatg aaggtcgtgt tccacatcca gagccagaac gtcgtttcgc
     7981 cgtgcagata cttgagatgc gcgagcgtcg ggttccaggt ccacagcggc ttgtactgcg
     8041 gccgcatcca cggaatgtag agctcattgt ccggccggac agtggtgata accatccaat
     8101 agaacggaaa gagcaggaac aggaggaata cgagcagggg gattcggaag aacacccacc
     8161 ggcgccatgc ggctccttcg atcatcagcg ggtctccacg cggcggatgt acatgagctg
     8221 gaagatgacg atcaggaaga gcagcgggaa catcgcgagc gagacggccg caccctgcga
     8281 caggaggccg gtgccgatgc cgagctgata ggcgtaggtc gcaaagaggt gcgtcgcatt
     8341 cgccggaccg ccgccggtca gcacgtagac gatctggaaa tcggcaaagg tctggatgac
     8401 cgagaacagc acgacgacca tcgtaacggg gagcagcagc ggccaggtga tatagatgaa
     8461 gcgctgccac ggcttggcgc cgtcgatcgc cgcggcttcc tgcaggtccg gcgagatcgt
     8521 ctgcaggccg gccatgagcg agatcgcgaa aaagggcacg ccgcgccaga cgttgacgat
     8581 gatgatcgag agcatggcga ggtcgccatc accgagccag ttgatcgagt tcgtgatgac
     8641 gccggccttc cagagaaacc agttcacaac cgagaacgtc ggatcgaaca tccacttcca
     8701 cgcgaagcag gcaagcacgg ttggcacgat gaacgggagc aggatgaagg cgcgcgtgaa
     8761 ggccttgaac ttgaagttcc ggttgagaag gatcgcgagc cagaggccga gtgccagctt
     8821 gaagatggtc gtcacgaatg tgtagaggaa cgtgttcttg acgacggcat ggaagatcgg
     8881 atcggcgagc aatcgcccga aattctcgaa gccgacccat ttgccgggct cgcccacctt
     8941 ggtgtcggtc accgcgagca tgatgccgcg caggaacgga taggcgatga agaggccgag
     9001 cagcaccagc gtcggcaaca gcaggagcag cgcaagattg ccctcgctct ccagcggtcg
     9061 ccatttgggc ttcgcgacga tgacatcgcc ggatggggct gcgggcacgg ccatgaggac
     9121 agctttcccg taagacttcg agtcgacagg ttcaagcgga gcgcgacgca cgcctggcgc
     9181 cacgcgcggc attcacccac gcacaatgaa accctcggcc ggtgacgtcc accggccgag
     9241 ggctccgtgg ttacgatcag gcgccgtaga tggtcttcag ctgagcctcg caggccttga
     9301 cagcctcctc ggccgcctgc cccttgatgg cggcagcgaa catgttgccg accagatact
     9361 tggtcagcac ctctgcggcc ttcgcattcg gctcgcccgg gtggccgggg gtggcgccga
     9421 gcttaccggc cacggcgaac ggtgccataa tcgggtcctt cgtccagatt ggatgagact
     9481 cccaacccgt ggtcgccggc gtgtagaagc ccatgccagt gctgaaccac tggtcgtagt
     9541 tctccttctt atggaagaag cgcagcagct ccttggcggc cttctggttc ttggagtagc
     9601 tcggcatgac gtgcgacgtg aaggtgtgca tgccgaactg gcctttcggg ccggcgggca
     9661 gcggcccgtg gaacacatcg tccttcaaag gcttcgcctc ggccgtcttg tactggtcgg
     9721 gcttgcgaag cgtctcgatg tagatcgagg cgccgttgag cgtcgccgag atcgtgccgg
     9781 cgaggaaggc gcggttgttg gaggcgtcgt cccaggcgag gccgccctcg tcgaacgact
     9841 ccttccaggc cgatgccatc cacttcacgg cgtcgaccgt ctccttagag ttgatgacga
     9901 ccttgccctt gtcgtcgatc tcggcgccgc cgtacgacca cagcagcggg taggcgaacg
     9961 tcggcgggtc gccgaacgac tggccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nntagatgtt catggcgccg atgatggccg ccggcatcga gaagaacttg ccgccagtgc
    10141 cgcagttcgc gctcgacacc ttgtagtagc cgctctgcgc cttgcccacc tcgtcggcga
    10201 cgtcggagag atcgacgagg ctctttgcgt agaggtgcgg gttgttgttg aagagcatga
    10261 tcaggtcggg accggcgccc gactggatcg aggaggtgat gcgcggctgg agatcgttgc
    10321 cgttgacggt ctcgtacttc accttgatgc cgagcgcctt ctcggcttct ggcagcagct
    10381 tcgtacgcag cagctggtcg caggccggga tgaagtcgtt ccagcgcagc cagtgaacct
    10441 ccgccgtctg ggcgaaggcc ggcgcgcggc cggtggccag aatgccggcc atgccgcccg
    10501 cggcggccgc cgatttcttc agaaaagttc tgcgggtcgt ccggctcatg gtttcctccc
    10561 ttgagtgccg aagcgttggg cggttgttgc ccgtattgcg ggcggacctt acgcgcccca
    10621 aatcaattca acaacggtct tgttgcgatc gtgaggcgaa ctcggggcca agtcccggca
    10681 agttccggct tggccccagc ggcaaacgag gctagaagac cggcgagatt tcggccggac
    10741 ggcgccctta gggcggcgca agtgccggca catggcgttc ctgaccgggg cgcatcacga
    10801 caggagtgtc cgcttggcca aggtgatcgc aagctccatc cgcaagggca atgtcctcga
    10861 gatcgacggc aagctctgcg ccgtcatgaa ggccgagaac atccaccccg gcaagggcac
    10921 gcccgtcacc cacctcgaga tgcgccgcat ctcggacggc atcaaggtgg tcgagcgcta
    10981 ccgcacgacc gagcaggtcg agcgcgtcta tctcgaggag gcgcaataca actacctgtt
    11041 cgaggacgac aacggcttca ccttcatgca tcctgagaac ttcgagcagg tgcaggtgaa
    11101 caaggacatc atcggcgacc agtccgtcta cctgcaggag aacatggtct gcacgctgca
    11161 gatctataac ggcgtgccag tctcctgcga attgccgccg cgcgtgacgc tcgaaattat
    11221 cgaggccgat ccggtcgtga aggggcaaac ggcggcctcg tcctacaagc cggccatcct
    11281 ctcgaacggt gtgcgcgtca tggtgccgcc gcacatctct tcggggacgc gcatcgtcgt
    11341 ctcgacggcc gacggcacct acgtcgagcg ctcgaaagag tgagggcagt cgtcagcaga
    11401 cgatgccgcc cgcgcggcgc gagcgcatac cgttgcccca cttggcgagc atggccgcga
    11461 cctcgtcggc cgaaaacggc ttggcgaggt agtcgtcgaa gccgctatcg aggcagtgct
    11521 ttcggtcctc ggggaatgcg ccggcggtca cggcgatgat cggcggcgcg atgcgcccgc
    11581 tcgatcggta aaggcttgcc agccgcgcgc gcgtctcgtg gccgtcgatc cctggcatgt
    11641 gaatgtcgag cagcacgagg tcgaaatgct gctcggccgc cgttacggcg ccgagccctt
    11701 cccgctcgcg aatgacaacg acgccgagcc gctcgagcaa ccggcacgtc agcagcgcgt
    11761 tgatgtcgtt gtcctcgacg agtagcacac ggcaatccgg aagcctgcag tccggacgta
    11821 ccggcccact cgggtccggc acgggtccga tcggcagggg cgcggcatcg accacgatct
    11881 gcgtgacgag cgactgcggg cgcaccggcc ggacgaggta ggcatcgaag ccgagcgtgc
    11941 gcagatgcgg caggcgatcc cgttcgctgg catcgatcac gatgatgccg cggggtcgca
    12001 atccgagcga gacttcccgg acgcgcttca ggatcgccgc cgccttctcc tcgccacggc
    12061 ccgtatcgag caccacggcc ttgatcggcg acatcgtcac gtggtcggcg acacgcacgt
    12121 cgatgacgcg cccgcccgcc cgcgcgatcg tcgccgtggt catggcctgc tcgatcgtcc
    12181 cggagagctc gacgacgatg atctcggagg ccagcgcctt cactggcaag gcggacgccg
    12241 ccggcatgcc cggcaccggg agcagcatcg tgaactcgga gccttcgccg agcttgctga
    12301 cgacctcgat ctcgccgccg agcgcccgtg cgaggctcct cgaaattgcg aggccgaggc
    12361 ccgagccgtc gatgcgacgc tggatggcgg tatctgcctg cacgaactcc gcaaacagcg
    12421 acgcgagcac ggcgggggcg atccccggtc ccgtgtcggc gatgcggaca gcgatcccct
    12481 cgccgcgccg ctcgatgcgc accgcgacgc cgcccgcgtc ggtgaacttt atcgcgttgc
    12541 caatgagatt gagcaggatc tggcggaccc gcatctcgtc ggtcgggatg gtcgccggca
    12601 ggtccgcgga catggtccag gcaagctcga ggcccttctc ggcggcattc ggggccagca
    12661 gctcgatcac accgccgacg cagtccgaga gcgagacggg ctcggcaagg agctccatac
    12721 ggcccgcttc aaccttggag agatcgagga tgccgttgac gagcttcagg agcgccttgg
    12781 cggagcggtc gacggcgtcg acataggtcg cctggtccgc gctgagcgag gtgtcgcgca
    12841 gcagcgtcgc catgccgagg ataccgttca tgggcgtgcg gatctcgtga ctcatcgagg
    12901 cgaggaagcg tgatttcgcc tcgctcgcgc tctgtgcctc gtcccgccgc cgcgcgagag
    12961 caatttcggc cgcgcgtgtc tcggtgatgt cgcggccgat gatctcgatg cctccgggga
    13021 ggccgtcgac atcgggcagc tccttgcggg accagaccag ccagcgcggc tcatccggcg
    13081 gtccgatggg tcggcagcaa tcgccaccgt cgcgcacggt ctcgggcaac tgtcccgcct
    13141 cgccgagcag gcgctcggcg gtgcggttgg catatgtgag attgccggag ggatcgcgtc
    13201 gcaggatggc atcgggatga tgatcgagca acgctcggag gcgggcctca ctttcgcgca
    13261 gcgaccactc ggcatcacga actgtctcgc tatccggcaa tggggcggcc ggtcgctcga
    13321 cctctacggt tcgagcccgg gccgcaagtg ccagggccag agccagaagg ctgcccgaga
    13381 ggataatcgc cacgtgcgcg atgccgatcg gcccggcgag ccgcgtcacc gaggccgcaa
    13441 gcattgcgta gcccgcaatc gcgacgaggc catgcggcgt gagacccaac tgctcgacag
    13501 tctggccggc gcgcgcaaac gcgcttgcgc gaccagcacg gggctgatcg ggctccggca
    13561 cgacggtgtc gcggtgctcg ctcggcatga acatcctgtc tgcgggcctt ggtgctcgct
    13621 tgccctgcac cctacgctcc gcatggttgc aattgccttc agcgggttgg ttagcgattg
    13681 atcctcggct aggctcattt cgagacacgc tgggaacgag gatggccatg ggaccgctga
    13741 agggcttcaa gatcatcgag atggccggga ttggcccggc gccgttctgc ggtatggtgc
    13801 tcgccgacat gggcgctgag gtgatccggg tcgaccggcg cgagggcgcg gatctggggc
    13861 tcccgggccg cgagccgaaa tacgatgtcc tccatcgcgg ccgccaatcg attgccgtcg
    13921 atgtccgcac ggaaggcggg cgcgaggtcg tgaaggcgct cgtcgcgaag gccgacgccc
    13981 tgatcgaggg gttccgcccg ggcgtgatgg agcgtctcgg gctcgggccc gacgatctcg
    14041 ccaaggtcaa tccgaagctc gtgttcggcc gcatgacggg attcggccag acaggcccaa
    14101 tgg
//
LOCUS       DAORNH010000092        14096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3256_length_14096_cov_2.640481, whole genome shotgun sequence.
ACCESSION   DAORNH010000092 DAORNH010000000
VERSION     DAORNH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3256_length_14096_cov_2.640481"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PK264_11080"
     CDS             complement(<1..217)
                     /locus_tag="PK264_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76464.1"
                     /translation="MRRTLQDQIRALDELSTLSRSEAVHRDIVAPMTAPAAAVEASPA
                     PLAPSRAAPAVRTAAAVGRLDGIAARPP"
     assembly_gap    254..273
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<274..>1002)
                     /locus_tag="PK264_11085"
     CDS             complement(<274..>1002)
                     /locus_tag="PK264_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76465.1"
                     /translation="DDAFQARLANFDDAILKSTLAIDSAVSEKARALTTALETHAKTI
                     SETLGRQANDFDESIVHGIQAVRRTSESVTRQSLKAIEGLVGQADVIKNVSENLLAQI
                     SSVANRFDKQGQTILHAAGALEAANYKIDSTLQTRQSELSRTLERLSTTADTVGDKLA
                     LTTETRSRAAIAELERFRTEADQSADGTIDLVRQKFDRLTTDFDQRIGSLSDRFSTTT
                     SDMRDRASRTLGEIEAEQNRLRIEL"
     assembly_gap    1003..1170
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1171..2350)
                     /locus_tag="PK264_11090"
     CDS             complement(<1171..2350)
                     /locus_tag="PK264_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76466.1"
                     /translation="MTSRHDSRGNLAIDDMATALASSAPEPVSQTSGPPPVPAPPNIL
                     STVSDDTLMPTDAPTSEAEAPVASPPALPRKAAGPRTRVAANDDAPSIGGLIHALQSR
                     PSRKPLTYAAAASGVWALLTVVLGGAMLGGELAKAASWLEVLARPTALILAATLLIPI
                     ALFWLLANLVMRTQELKSMSSAMTEVAVRLAEPDRMAEQQIASLGQSIRRQVSYMNNA
                     VSHALGRANELEALVHNEVAALERAYTDNEARIQRLIKELSTERHALLSTGDNVSETL
                     RNLGTEIPSMIDRLGEQQIRLARFIEGAGQNLVALESTLASATDRIEGSLGARVTTLT
                     DALEKHQGWIDTEIGQKTATLQTMLDDHSGKVQTALADRTGQLQSVFSEFTEALNHAL
                     G"
     gene            2675..3046
                     /locus_tag="PK264_11095"
     CDS             2675..3046
                     /locus_tag="PK264_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRD76467.1"
                     /translation="MTSAAAALRDMPTIDLEHLRRFTRGDEPLICEVLQLFANQAPHY
                     LKEMRATASDRGWFEAAHALKGCSRAVGAWELARLAEMAERLRDGASEHCQGQRRALM
                     PEIERTLAAAIDFARATTRRR"
     gene            complement(3053..3919)
                     /locus_tag="PK264_11100"
     CDS             complement(3053..3919)
                     /locus_tag="PK264_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HRD76468.1"
                     /translation="MLDRSPTPPILASIAHLASGYHAWISDIWGVLHNGIARFPRASE
                     ACVTFRRSGGRIVLVTNAPKPAWFVERMLDRLEVPREAYDAIITSGDVTRDLIAPIAA
                     RGIYHLGPDYDRPIFEGLPVRFAAVEDAEVVVCSGLFDDDRETPADYAGMLDRLKARN
                     LLMICVNPDLVVERGERIVYCAGALAEAYEKIGGRVAYAGKPHRAIYETAMRSLADLA
                     GAQMPPDRILAIGDGIRTDMRGAATMGLDSLFIASQLHVPAGRTMDEALLGDLFKAEA
                     TKPVAAMSGLAW"
     regulatory      3997..4075
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            4251..5369
                     /locus_tag="PK264_11105"
     CDS             4251..5369
                     /locus_tag="PK264_11105"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRD76469.1"
                     /translation="MTFGADEALLLDCKQLLAPFQVAYMTYGTLNANKSNAILVCHAL
                     TGDQYVASPHPVTGKPGWWTTMIGPGKPVDTNKYFVICSNVIGGCMGSTGPASINPKT
                     GKPWGLDFPVITIGDMVDAQVRLIDRLGIGQLFAVIGGSMGGMQVLEWAARHKERVYT
                     AVPIATAAWHSAQNIAFHEVGRQAVMADPEWAGGAYLEKSRVPRNGLAVARMAAHITY
                     LSEAALQRKFGRNLQDRAAKTFSFAPDFQVESYLRHQGSTFVDRFDANSYLYITRALD
                     YFDLAAEHGDVLANAFRGTETRFCVVSFTSDWLYPTHESKEIVQALNAVAANVSFVEV
                     QSDKGHDAFLLEEPEFFATITGFLNAAAAKKGIAVRRG"
     gene            5504..7855
                     /locus_tag="PK264_11110"
     CDS             5504..7855
                     /locus_tag="PK264_11110"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRD76470.1"
                     /translation="MASYTGTTGNDTVTGTADADTFNMDAGKDVVRSGAGNDTIMLGP
                     GDDWANGEIGDDVLHGEDGNDTLTGDAGNDTIYGGAGNDGVYGGGNNDLVYGGDGNDN
                     LNGDGANDVVFGEAGDDKVNGGTGDDRLDGGFGNDAIDGGSGNDTIVVRAGEGVDTIN
                     GGQGVDAIELRLTSADLTPALRTDLRGLVTWMDGNLAAAGGSMTTLASAATGATFTVG
                     ALGVTVSTIETINVYLDGNPVPLADILNRAPSVLPEQMLAGSEDTVISGGVGASDPDG
                     DALSYVIASGPAHGAIALDPATGLFTYTPDANWSGNDSFSVVVADPLGLSATQTVSLS
                     VAATADVPSLAVVTPVIVPAGEIKLGLGSDDHLAGGAGSDFLHGGDGNDVVDGGAAVA
                     IKTQLDITAALGDLDGSELLSVTLSNLPAGSVLSAGAANADGSWTVGADDLAGLTLSA
                     TVDRAFSISVTATASEASGSTASAAATIDVVLGRDTLIGGKGSDTVLGGAADDALYGG
                     NRPGGTPSLPRAPTAADNDVVRGGDGNDMVYGNHGDDELFGDAGNDKLYGGKGNDLVD
                     GGDGDDLLHGNSGEDTLRDGAGNDIVSGHSGNDLMVAGEGDDSYSGGSGFDTLDFSAA
                     LGGVNIDISKKVASGMGNDKFSSVESFVGSVHDDTFKGSSAVDTIDGGAGNDLIRGLG
                     GADVLSGGEGADHFFWEKLDVSASKGFDRITDFEVGDVLDFTKLVSVGSKPIDDFVRV
                     TDTSAGSLVTVKLGGAMVTVALLEDVHGATAASLAQDGQLLLG"
     gene            7967..8845
                     /locus_tag="PK264_11115"
     CDS             7967..8845
                     /locus_tag="PK264_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76471.1"
                     /translation="MADSSKGLGIVPVVAAAVIAAAAGAGGYHWLMRPSAPPAPASAT
                     RSTAAVAKPDPAPTPAPAVAPTPEKPKVSEAEALRLKVFAAHGGEKAAAKRALSAAFA
                     SETRDGKVFARGRIWWRAPDARAIEFDVPAGPAGGAATKHRIVFDGKRAGFQGGKTVT
                     PELTELMRLDTRFFEPADWATLYRSVELGGEETLGSEPALKVIKVSTGSMRVIDYYAT
                     RTSLLVARDTAEYDAGSKSFKALHLRYSDHRPVDGIPVPHTITTLDDSKSVLTVMRLT
                     GFSWDGPIPDGTFTMP"
     gene            8881..10272
                     /locus_tag="PK264_11120"
     CDS             8881..10272
                     /locus_tag="PK264_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HRD76472.1"
                     /translation="MAPIRHTIGATRFVFRDLAELMAKATPRRSGDELAGVAAESDEQ
                     RVAAQMALADLPLRFLAEPLVPPETCEVSRLIADSHNAEAFAPVAHLTVGGFRDWLLS
                     REASEAALAALAPGITPEMAAAVSKIMRNQDLILAARKCRVVTLFRNTIGLSGRIATR
                     LQPNHPTDDPAGIAASIFDGLAYGNGDAVIGINPATDNVAAVMTLLEMLDRVISEFAI
                     PTQACVLAHATTTLECLRRGAPVDLVFQSIAGTEAANRSFGVSLALLAEAREAALALK
                     RGTIGENVMYFETGQGSALSANAHHGLDQQTAEARAYAVARQFSPLLVNTVVGFIGPE
                     YLYDGKQIIRAGLEDHFCGKLMGVPMGVDICYTNHAEADQNDMDTLLTLLGVAGCSYI
                     MGIPGSDDVMLNYQTTSFHDALYLREVLGLRPAPEFEAWLQANGIFEAGERPRLADRL
                     PSLLAAPLSKVEA"
     gene            10312..11145
                     /gene="eutC"
                     /locus_tag="PK264_11125"
     CDS             10312..11145
                     /gene="eutC"
                     /locus_tag="PK264_11125"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HRD76473.1"
                     /translation="MAGAAPGPSVIADPWTALRRLTTARIALGRAGVSLPTRAHLEFQ
                     LAHARARDAVHEALAVAPLAAAVEALGLTTLALRSAAPDRTLYLQRPDLGRTLDPASR
                     TLLERRAAARPDTPRRRRPSADLAIVIADGLSALAVARNAAPLISAVLPHATREGWTL
                     APVAIVEQGRVAIGDEIGALIGARAAVVLIGERPGLSSPDSLGVYLTFDPKPGRTDAE
                     RNCISNIRKGGLAPASAARTLAYLVREALARKFTGVELKDESAPPPSAIRKAPRNFLL
                     E"
     gene            11200..12000
                     /locus_tag="PK264_11130"
     CDS             11200..12000
                     /locus_tag="PK264_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRD76474.1"
                     /translation="MPQDIAPPSALAFPPLDRSVTKAEWAARVDLAACYRLVDHFDMS
                     DMIYTHISVRHPERPDRFLLNQHGLLFGEMTASSFLEVDVTGKIHRHPDPSRGYGLHA
                     AGFVIHSAIYKARPDVGAVLHTHTIAGMALSSLACGLTPLTQTSHRFYGRVAYHDFNG
                     PERDPAEREKLAKSLGSLDVMILRNHGLLTVGPTVPEAFNRMYGLERAAKAQLAAMAC
                     NTPLEMPSDDVIAKSTAMYAPGATRSYGILEWPGLLRLLDRNDPSYRS"
     gene            12130..13344
                     /locus_tag="PK264_11135"
     CDS             12130..13344
                     /locus_tag="PK264_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76475.1"
                     /translation="MVTDVSRPAEVRDRRELATLSSVCLAHWVSHVHILVLALLIPLL
                     NKELGVGFLDLGVAITIFSIVSGLTQAPVGFLVDRLGPRRILTLGLCLGGLAFIVLGL
                     ALSYPALLACAAIAGLANSVYHPCDYAILAAGIDDKRMGRAFSIHTFSGFLGSAMAPG
                     MVLFAVSWFGIKAALVLMGLLGPLAAAILLLVPDVVAKPAATATGKAAGSLASVLSPA
                     IIALMVFFTLLSLSTSGISNFAVSALNLAYDVSFSAANVALTGFLGAGALGVLAGGYL
                     ADRTTRHGQVAAACFTANAVIVLLIGLLPMHIIVLVPTLTLAGFLWGIIAPSRDMLVR
                     KAAPPGAAGRAFGIVSTGFNIGGIIGPLLYGWIMDMKMPRTVFLVAAAFMIATVLLAL
                     VTDHRQSRATAN"
     gene            13505..>14096
                     /locus_tag="PK264_11140"
     CDS             13505..>14096
                     /locus_tag="PK264_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRD76476.1"
                     /translation="MPAKRIICVGHAALDRIYRISEFPKAPTKVRALEHVEVGGGMAA
                     NAAVAIARLGGKSELWSRVGDDAAGQTIRAGLRAEKVDIRYVHAFEDARSSTSAIIVD
                     DHGERLIVGQRDITTPTSTSWLPLERVTEADAVLGDVRWLEALRAVFTRARDAGVPTI
                     LDGDVGAREALPEILPLVDYAVFSATALRELEAGGTD"
BASE COUNT         2289 a         4834 c         4475 g         2310 t
ORIGIN      
        1 tcggcgggcg ggcggcgatc ccatccagcc gccccacggc agccgcagtc cggaccgctg
       61 gagccgcacg cgaaggtgcc agtggcgccg gcgatgcctc gacagccgcc gcaggtgccg
      121 tcatcggcgc gacgatatcg cgatgcacgg cctcgctgcg cgaaagggtc gacaactcgt
      181 cgagcgcgcg gatctggtcc tgcagcgtgc gtcgcattcc ctccgtgctc gcgcgcgtcg
      241 cctccggcag gcgnnnnnnn nnnnnnnnnn nnnagctcga tgcgcagccg gttctgctcc
      301 gcctcgatct cgccgagcgt gcgcgacgcc cgatcacgca tgtcgctagt ggtcgtggag
      361 aagcgatcag agagcgagcc gatgcgctgg tcgaagtcgg tggtcaggcg gtcgaatttc
      421 tgcctgacga gatcgatggt gccgtccgcg gactgatcgg cctcggtacg gaagcgctcg
      481 agctcggcga tcgccgcgcg cgagcgcgtt tcggtggtga gcgcgagctt gtcaccgacg
      541 gtgtcggccg ttgtcgacaa gcgttcgagc gtgcgactga gctcgctctg gcgggtctgc
      601 agggtcgaat cgatcttgta gttggcggcc tcgagggcgc ctgcagcatg caggatcgtc
      661 tggccctgct tgtcgaagcg gttggcgacc gagctgatct gggcgagcag gttctcggac
      721 acgtttttga tgacgtcggc ctggccgacg aggccttcga tcgccttgag cgactggcgg
      781 gtgacgctct cgctcgtccg ccgcacggcc tggatgccat gcacgatcga ttcgtcgaag
      841 tcgttcgcct gacggccgag tgtctcgctg atggtcttgg cgtgtgtctc gagcgcggtc
      901 gtgagtgcgc gcgccttctc gctgacggcg ctgtcgatcg cgagcgtcga tttcagaatg
      961 gcatcgtcga agttggcgag ccgcgcctgg aaagcgtcgt cgnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgccgagcgc gtggttgagc gcctcagtga
     1201 actcggagaa gaccgattgc agctggccgg tgcgatcggc aagcgctgtc tggaccttgc
     1261 ccgagtggtc atcgagcatg gtctgcagcg tcgcggtctt ctggccgatc tcggtgtcga
     1321 tccagccctg gtgcttctcg agtgcgtcag tcaaggtcgt gacacgggcg ccgagcgagc
     1381 cttcgatgcg gtcggtcgcg ctcgcaagcg tcgactcgag tgcgacgagg ttttgcccgg
     1441 caccctcgat gaagcgggcg agccggatct gctgctcgcc gagccggtcg atcatcgagg
     1501 ggatctcggt gccgagattg cgcagcgtct cgctgacatt gtcgccggtc gagagcagcg
     1561 catgccgctc ggtcgatagc tccttgatga ggcgctggat gcgcgcctcg ttgtcggtat
     1621 aggcgcgctc gagggcggcg acctcgttgt gaaccagcgc ctcgagctcg ttcgcccggc
     1681 cgagcgcgtg cgagacggca ttgttcatgt aggaaacctg gcgccggatc gactgcccga
     1741 gcgaggcaat ctgctgctcg gccatgcgat cgggctcggc gagccgcacc gcgacctcag
     1801 tcatcgccga ggacatgctt ttcagctcct gggtgcgcat gacgaggttt gcgagcagcc
     1861 agaagagggc gatgggaatg agcagcgtcg ccgccaggat gagcgccgtc ggccgcgcga
     1921 gcacctcgag ccagctcgcc gccttggcaa gctcgccgcc gagcatggca ccgccgagaa
     1981 caacggtcag aagggcccag accccgctgg cagcggcggc atacgtcaac ggcttgcgcg
     2041 acggtcgcga ctgcagcgcg tggatgaggc cgccgatgga cggcgcatcg tcgttggcgg
     2101 caacccgtgt ccgtggcccc gccgccttgc gaggcaaagc aggcggcgat gccactggtg
     2161 cctcggcctc ggaggtcggc gcatcggtcg gcatcagcgt gtcgtctgac acggtcgaca
     2221 gaatattcgg tggagccgga acaggtggcg ggcccgatgt ctggctcaca ggctcgggcg
     2281 cgctcgacgc gagtgcggtc gccatatcgt cgatggcgag gttgccgcgt gaatcatgcc
     2341 tactcgtcat tcagagccgc ccagcactcg aggggtcgat ctcgcggtcg agacagaccg
     2401 ccccacatcg cccagcctta gagcatagca tgcccttagc cgagctgccg gcactggaat
     2461 agtgtgaata agcatccccc atccacccag tagagctgtc gttcttctcc caccgatcat
     2521 cccgccgcca gagaagggtg cggtccgagt acggcaaaag caagtcctgc gaaggtagtt
     2581 gccggatcaa cttagagaaa gattaacacc agacgagcat aatgccgctc caagaccact
     2641 gtatgtgacg acgacaagct cgtggagagg catcatgacg agtgctgctg cggccctccg
     2701 ggacatgccg acgatcgatc tcgagcacct ccggcgcttc acgcgcggcg acgagccgct
     2761 gatctgcgag gttctgcaac tttttgcaaa tcaggccccc cactacctga aagagatgcg
     2821 cgcgacggca tcagatcgcg gctggttcga ggccgcccat gccttgaagg gctgctcgcg
     2881 tgccgtcggc gcctgggagc ttgcccgtct cgcagagatg gcggagcggc tccgcgacgg
     2941 tgcctccgag cattgccaag gtcagcgtcg cgccctcatg cccgagatcg agcgcacgct
     3001 ggcagcggcg atcgactttg ccagggcaac cacgcggcgc aggtaaccga actcaccaag
     3061 cgagcccgct cattgcagcg acaggcttcg tcgcctcggc cttgaagagg tcaccgagca
     3121 gcgcctcgtc catcgtccgg cctgccggca catgcagctg gctcgcgatg aacaggctgt
     3181 cgagacccat cgtcgccgcg ccgcgcatgt cggtgcggat gccgtcgccg atggcgagaa
     3241 ttcgatcggg cggcatctgc gcgcccgcca gatctgcgag agatctcatg gccgtctcgt
     3301 agatcgcccg atgcggcttg ccggcatagg cgacacgccc gccgatcttc tcgtatgcct
     3361 cggcaagggc ccccgcgcag tagacgatcc tttcgccgcg ctcgaccacc agatcagggt
     3421 tcacgcaaat catcaggagg ttgcgggcct tcaggcgatc cagcatgccc gcatagtcgg
     3481 ccggcgtctc acgatcgtcg tcgaagaggc ccgaacaaac caccacctcg gcgtcttcca
     3541 ccgccgcaaa gcgcacaggc agcccctcga agatgggtcg atcgtaatcg gggccgagat
     3601 ggtagatgcc cctggcagca attggcgcga tgaggtcgcg ggtgacgtcg cccgacgtga
     3661 tgatcgcgtc ataggcctcg cgcggcacct cgaggcggtc cagcatgcgc tcgacgaacc
     3721 aggcaggctt cggggcattg gtgacgagga cgatgcgtcc gccggaccgg cggaaggtga
     3781 cgcaggcctc gctcgcccgc gggaaacggg cgatgccgtt gtgcagcacg ccccagatgt
     3841 cagaaatcca ggcgtgatag ccggacgcaa ggtgagcgat cgaggcgagg atcgggggcg
     3901 tcggtgatct gtcgagcatg ccgcggggta acgcctttgc gccggttgtt caagcgtgcg
     3961 ggtgaaactt gacggggggg ctcacttatt attacgtgcc gccatcgtgg tcatttggcc
     4021 ggccggcttg cagccacgtt aaacaactcg ctaaagaggc cgtgcgattg agaccctcga
     4081 cgggatcgcc ggctccgatg gaggcagcta tggcgtcgga tcgcacacat caggtcgacc
     4141 gcacggatcg agacgtacgc gctgcggccg acagcgatgt cgtgctcgag actgccgccg
     4201 cccgcccgct cgcccgctcg ccggaggtcg acacgccgtc gagcccggtg atgaccttcg
     4261 gcgcggacga ggcgctgctg ctcgattgca agcagctgct cgcgcccttc caggtcgcct
     4321 acatgaccta cggcacgctg aacgccaaca agtcgaacgc gatcctagtc tgtcatgcgc
     4381 tgaccggcga ccagtatgtg gcgagccctc atccggtgac gggaaagcca ggctggtgga
     4441 cgaccatgat cgggccgggc aagcccgtcg ataccaacaa gtacttcgtc atctgttcga
     4501 acgtgatcgg cggctgcatg ggctcgaccg gccccgcctc gatcaacccg aagaccggga
     4561 agccctgggg cctcgatttt ccggtgatca ccatcggcga catggtggac gcgcaagtgc
     4621 gcctcatcga ccggctcggc atcggccagc tgttcgccgt gatcggcggc tcgatgggcg
     4681 gcatgcaggt actcgagtgg gcggcgcgtc acaaggagcg cgtctatacc gccgtgccga
     4741 tcgcgaccgc cgcctggcat tccgcgcaga acatcgcttt tcacgaggtc gggcgtcagg
     4801 ccgtgatggc cgatcccgaa tgggccggcg gcgcctatct cgagaagagc cgcgtgccgc
     4861 ggaacggtct cgcggttgcc cgcatggccg cccacatcac ctatctctcg gaagccgcgt
     4921 tgcagcgcaa attcggccgc aacctgcagg accgggccgc gaagacattc tcgttcgcgc
     4981 ccgatttcca ggtcgaaagc tacctgcgcc accagggctc caccttcgtc gaccggttcg
     5041 acgccaacag ctacctctac atcacgaggg cgctcgacta cttcgatctg gcggccgagc
     5101 atggcgacgt cctcgccaac gccttccgcg gcaccgagac gcgcttctgc gtcgtctcgt
     5161 tcacgtccga ctggctctat ccgacgcacg aatcgaagga gatcgtgcag gcactgaacg
     5221 cggtcgccgc caacgtctct ttcgtcgagg tgcagagcga caagggccac gatgcattcc
     5281 tgctcgagga gcccgagttt ttcgcgacca tcaccggctt cctcaacgcg gccgccgcaa
     5341 agaaggggat tgcagtgcgg cgcggctgag accgcgccgg gctccgacga ttgataaaat
     5401 tcgaacgaat ttcgcagcga atacccttgc cgcttcgaac ataatctttt gatttctcgt
     5461 ctagcgtggc ctgatcggaa ctctgccgag gggctgctct gtcatggcga gctacacggg
     5521 aacgactggc aacgacaccg tcacggggac ggccgatgcc gacacgttta atatggatgc
     5581 cggtaaggat gtcgtgcgct cgggcgccgg caacgacacg atcatgctcg gccccggcga
     5641 cgactgggcc aacggcgaga tcggcgacga cgttctccat ggcgaggacg gcaacgatac
     5701 gctgacgggc gacgccggca acgacacgat ttacggcggc gccgggaacg acggcgtcta
     5761 cggtggcggc aacaacgacc ttgtctacgg cggcgacggc aacgacaacc tcaacggcga
     5821 cggcgccaac gacgtcgtct tcggcgaagc cggtgacgac aaggtcaacg gcggcacggg
     5881 cgacgatcgc ctcgacggcg gcttcggcaa cgacgccatc gacggcggat cgggcaacga
     5941 cacgatcgtg gttcgcgcgg gtgaaggcgt cgacacgatc aacggcggac agggcgtcga
     6001 tgcgatcgag ctcaggctga cgagcgccga cctgacaccg gcgctgcgca ccgatctccg
     6061 cggcctcgtc acgtggatgg acggcaacct cgccgccgcc ggcggcagca tgaccacgct
     6121 cgcctcggcc gcgaccggcg caacattcac ggtcggcgca ctcggcgtta ccgtctccac
     6181 catcgagacg atcaacgtct acctcgacgg caacccggta ccgcttgccg acatcctcaa
     6241 ccgcgcgccc tccgtgctgc ctgagcagat gctcgccggc agcgaggata cagtgatctc
     6301 gggtggcgtc ggcgcctcgg accccgacgg cgacgcgctg agctatgtga tcgcctccgg
     6361 gcccgcccat ggcgcgattg ccctcgaccc ggcgaccggc ctcttcactt acacgccaga
     6421 tgccaactgg tcgggcaacg acagcttctc cgttgtcgtc gccgacccgc tcggcctcag
     6481 cgcgacgcag actgtctccc tctctgtcgc cgcaaccgca gacgtcccgt cactggccgt
     6541 ggtcacgcct gtgatcgtgc ccgcaggcga gatcaagctc ggcctcggca gcgacgatca
     6601 cctcgcaggt ggtgccggca gcgacttcct gcacggcggc gacggcaacg acgtcgtgga
     6661 cggcggagca gcagtcgcca tcaagaccca gctcgacatc acggcagccc tcggcgatct
     6721 cgacggctcg gagctgctct ccgtcacgct ctcgaacctt ccggcgggct ccgtgctgtc
     6781 cgccggagcc gccaacgcgg acggcagctg gactgtcggc gcggacgacc ttgcgggcct
     6841 gacgctcagc gccactgtcg atcgcgcctt ctcgatctcc gtcacggcga cggcgagcga
     6901 ggcgagcgga tctaccgcat cggccgcagc caccatcgac gtcgtgctcg gccgcgacac
     6961 gctcatcggc ggcaagggca gcgataccgt gctcggcggt gccgcagacg atgcgctcta
     7021 cggcggcaac cgcccgggcg gcacgccgtc cctgcctcgc gccccgaccg ccgccgataa
     7081 tgacgtcgtc cgtggcggcg acggcaacga tatggtctac ggtaatcacg gcgacgacga
     7141 actcttcgga gatgccggaa acgacaagct ctacggcggc aaaggcaacg acctcgtcga
     7201 cggcggtgac ggcgacgacc tcctgcacgg caactcgggg gaggacacgc tccgcgacgg
     7261 cgccggcaac gacatcgtca gcggccacag cggcaacgac ctcatggtgg ccggcgaggg
     7321 cgacgacagc tattccggcg gcagcggctt cgacacgctc gatttctcgg ctgccctcgg
     7381 cggcgtcaac atcgacatct cgaagaaggt cgcgtccggc atgggcaacg acaagttctc
     7441 gagcgtcgag agcttcgtcg gatcggttca cgacgacacc ttcaaggggt cgagcgcggt
     7501 cgataccatc gacggcggcg ccggcaatga tctcatccgc ggcctcggcg gcgctgacgt
     7561 gctctcgggc ggcgagggtg ccgaccactt cttctgggag aaactcgatg tcagcgcctc
     7621 caagggcttc gatcgcatca cggacttcga ggtcggagac gtcctcgact tcaccaagct
     7681 cgtgagcgtc ggctcgaagc cgatcgacga tttcgtccgc gtcacagata cgtccgcggg
     7741 ctcgctcgtc acggtgaagc tcggcggcgc gatggtgacc gtcgccctcc tcgaggatgt
     7801 ccacggcgcc acggctgcga gcctcgccca ggacggccag ctgctcctcg gctgacggct
     7861 ccggcagcac actccgcctc gtcatcgctc ggcactccac cgttcgggcg attgctcttc
     7921 gctgcgcctc atgtactctc gcgccgaggc gggccgggag gcgagaatgg cggatagcag
     7981 caaggggctc ggcatagttc cagtggtggc ggcagcggtg atcgcggccg cggctggcgc
     8041 cggcggttac cattggctga tgcggccgag tgccccacca gcaccagcat cggcgacgcg
     8101 ctcgactgct gcggtcgcga agccggaccc ggctcctact ccggctccgg cagttgcgcc
     8161 gacaccagag aagccaaagg tgagcgaagc cgaggcgctc cgcctcaagg tctttgcagc
     8221 gcacggcggc gagaaggctg ccgcgaagcg cgcgctctcg gcagccttcg cctccgagac
     8281 gcgcgacggc aaagtattcg cgcgcggccg catctggtgg cgtgcgcccg atgcccgcgc
     8341 gatcgagttc gatgtcccgg ccggacctgc cggcggcgcg gcgacgaagc atcgcatcgt
     8401 cttcgacggc aagcgtgcgg gcttccaggg cggcaagacg gttacgcccg agctgaccga
     8461 gctgatgcgc ctcgataccc gcttcttcga gcccgcggac tgggcgacgc tttaccggtc
     8521 ggtcgagctc ggcggagagg agacacttgg cagcgagccg gcgctcaagg tgatcaaggt
     8581 ctcgacgggt agcatgcgcg tgatcgacta ttacgcgacc aggacgagtc tgctcgttgc
     8641 ccgcgatacg gccgagtacg atgcaggatc gaagtcgttc aaagcgctgc atctgcgcta
     8701 ctccgaccat cggccggtgg acggtatccc tgtgcctcat acgatcacca cactcgacga
     8761 tagcaagagt gtgctcacgg tcatgcggct cacgggtttc agctgggatg gccccattcc
     8821 ggatggcacg ttcacgatgc cctgaggctg ctaactgtcg acgctctgcc gggaggagcc
     8881 atggccccca tccgccatac gatcggcgca acgcgcttcg tctttcggga cctcgcggag
     8941 ctgatggcca aagcgacacc ccgccgatcg ggtgacgagc tcgccggcgt cgccgccgag
     9001 agcgacgagc agcgcgtcgc cgcgcagatg gcgctcgccg acctgccgct ccgtttcctc
     9061 gccgagccgc tcgttccacc ggagacatgc gaagtctcgc gcctcatcgc cgacagccac
     9121 aatgccgaag ccttcgcgcc ggttgcacac ctgaccgtcg gcggcttccg cgactggctg
     9181 ctctcgcgcg aggcgagcga ggccgcactt gcagcactcg cacccggcat cacgcccgag
     9241 atggccgccg ccgtctccaa gatcatgcgc aaccaggatc tgatcctcgc cgcccgcaag
     9301 tgccgcgtgg tgaccctatt ccgcaacacc atcggcctct ccggccgcat cgccacgcgg
     9361 ctccagccca atcacccgac ggacgatccg gccggcatcg ccgcctccat ctttgacgga
     9421 ctcgcctacg gcaacggcga tgccgtgatc ggtatcaatc cggcgaccga caatgtggcc
     9481 gcggtgatga cgctgctcga gatgctagat cgtgtgatca gcgagttcgc gatcccgacg
     9541 caggcgtgtg tgctcgcgca tgcgacgaca acgctcgaat gcttgcgccg cggcgcgccg
     9601 gtcgatctcg tcttccaatc catcgccggc accgaagccg cgaaccggtc gttcggcgta
     9661 agcctcgcgc tgctcgccga ggcgcgcgag gcagcccttg cattgaagcg tggcacgatc
     9721 ggcgagaatg tcatgtattt cgagacgggg caggggagcg cgctctcggc caacgcgcat
     9781 cacggcctcg accagcagac cgcggaggcg cgcgcctacg ccgtcgcccg gcagttctcg
     9841 ccgctgctcg tcaacaccgt cgtcggcttc atcggtcctg agtacctcta cgacggcaag
     9901 cagatcatcc gcgccggcct cgaggaccac ttctgtggca agctcatggg cgtgccgatg
     9961 ggcgtcgaca tctgctacac caaccacgcc gaggccgacc agaacgacat ggacacgctg
    10021 ttgaccctcc tcggtgtcgc cggctgctcc tatatcatgg gcattcccgg ctcggacgac
    10081 gtcatgctca actatcagac gacgtcgttc catgatgcgc tgtatcttcg tgaagtgctc
    10141 ggcctgaggc ccgcgccgga gttcgaggcc tggctgcagg cgaacggaat cttcgaggca
    10201 ggcgaacggc cgcggctcgc cgaccgtctg ccgagcctgc tcgcggcgcc actcagcaag
    10261 gtcgaggcct gagccgtggc gcgcgctccg aaatcgcggc agtcgacgcc cgtggcaggc
    10321 gccgcgccgg gaccatccgt catagccgat ccctggacgg cgctccgccg cctgacgacc
    10381 gcacgcatcg ccctcggccg ggccggcgtc agcctaccga cacgcgcgca cctcgagttc
    10441 cagctcgccc acgcccgcgc ccgtgatgca gtgcatgagg cactcgccgt cgcgccactc
    10501 gctgccgcag tcgaggcgct cggactcacg acccttgcgc tcaggagtgc tgcgcctgac
    10561 cgcactctct atctgcagcg gcccgatctc ggacgcacgc tcgatccggc gtcgcgtacg
    10621 ctccttgaac gacgtgcggc tgcgcgccca gacacaccca ggcggcggag gccctccgct
    10681 gacctcgcca tcgtcatcgc cgacggcctc tccgcgctcg cggtcgcccg caatgccgca
    10741 ccgctgatct ccgcggtcct gccgcatgcg acacgcgaag gctggacgct cgcgccggtc
    10801 gcgatcgtcg agcaggggcg cgtcgccatc ggcgacgaga tcggtgcgct gatcggtgcc
    10861 cgggccgccg tcgtactgat cggcgagcgt ccgggcctct cctcgcccga cagcctcggc
    10921 gtctacctca ccttcgaccc gaagcccggc cgcacggatg ccgagcgcaa ctgcatctcc
    10981 aatatccgca agggcggcct cgcgcccgcg tccgccgccc gaacgctcgc ctacctcgtc
    11041 cgcgaggcgc tcgcccgcaa gttcaccggc gttgagctca aggacgagag cgcgccgcca
    11101 ccgtccgcca tccgcaaggc gccgcgcaac tttcttctcg agtgacggca cgccgccgca
    11161 gagttagcct gcggcgacta cagtgggccg aggaccgcca tgccgcagga tatcgcgccg
    11221 ccgagcgcac tcgcattccc gccgctcgat cggagcgtca ccaaggccga gtgggcggcc
    11281 cgcgtcgacc tcgccgcctg ctatcggctc gtcgatcatt tcgacatgtc ggacatgatc
    11341 tacacccaca tctccgtgcg ccatcccgag cggccggacc gtttcctcct caatcagcac
    11401 ggcctcctgt tcggcgagat gaccgcctcc tcgttcctcg aggtggatgt caccggcaag
    11461 atccaccgcc accccgaccc atcgcgcggc tacggcttgc acgccgccgg attcgtcatt
    11521 cacagcgcga tctacaaggc acggccggac gtcggcgccg tgctgcacac gcacaccatt
    11581 gccggcatgg cgctttcctc gctcgcctgc ggtctgacac cactgactca gacctcgcac
    11641 cgcttctatg gccgtgtcgc ctaccacgac ttcaacggcc ccgagcgcga tcccgccgag
    11701 cgcgaaaagc tcgccaagag cctgggcagc ctcgacgtga tgatcctgcg caaccacggc
    11761 ctcctgaccg ttggaccaac tgtccccgag gccttcaatc gcatgtacgg cctcgagcgc
    11821 gccgccaaag cgcagctcgc cgccatggcc tgcaatacac ccctcgaaat gccctcggac
    11881 gacgtcatcg ccaagtcgac ggccatgtac gctcccggcg ccacacgatc ctatggcatt
    11941 ctcgagtggc ccggcttgtt gcgcctcctc gaccggaatg atccctccta ccggagctga
    12001 ccggcgcttc agccgacgca gcgccgtcgg cgtgactgca cgtcgcctat gcgccagcgc
    12061 acctttacag ccgcggcgtc gcatccgatg acagacgctc gattccctcc gagctgaggc
    12121 cgccacgcga tggtcacgga cgtctctcgc cccgccgaag tccgcgaccg tcgggagctc
    12181 gcaacgctga gctccgtctg cctcgcgcac tgggtgagcc atgtgcacat cctggtgctg
    12241 gcactgctca tcccgctcct caacaaggaa ctcggcgtcg gcttcctcga tctcggtgtc
    12301 gcgatcacga tcttcagcat cgtctcggga ctgacacagg cgccggtcgg cttcctcgtc
    12361 gaccggctcg gaccgcggcg catcctcacc ctcggcctct gcctcggtgg gcttgccttc
    12421 atcgttctcg gcctcgcatt gagctatccg gcgctgctcg cctgcgccgc catcgccggc
    12481 ctcgccaaca gtgtctacca cccctgcgac tacgccatcc tcgccgcggg catcgacgac
    12541 aagcgcatgg gccgagcatt ctcgatccac accttctcgg gtttcctcgg cagcgctatg
    12601 gcgccaggta tggtgctctt cgccgtgtcg tggttcggga tcaaggcggc attggtgctc
    12661 atgggtctgc tcggcccgct cgccgccgcg atcctgctgc tcgtgccgga cgtggtcgca
    12721 aagccggcag ccactgcgac aggcaaggcg gccggctcgc tcgcgagcgt gctgtcgcct
    12781 gcgatcatcg cgctcatggt gttcttcacg ctgctgagcc tatcgaccag cggcatctcg
    12841 aactttgccg tatcagcgct gaaccttgcc tatgacgtct cattctcggc tgccaatgtg
    12901 gcactgactg gctttctcgg cgccggagcg ctcggcgtac tcgcgggcgg ctatctcgcc
    12961 gacaggacga cgcggcatgg ccaggtcgct gccgcctgct tcaccgccaa cgccgtcatc
    13021 gtgcttctca tcggcctgct gccgatgcac atcatcgtgc tcgttccgac gctgacgctc
    13081 gccggattcc tgtggggcat catcgcgccc tcgcgcgata tgctcgtgcg caaggcggcg
    13141 ccgcctggag ccgcaggtcg ggccttcggc atcgtctcga ccggcttcaa tatcggcggc
    13201 atcatcggcc cgctgctcta cggctggatc atggacatga agatgccgcg gacagtgttt
    13261 ctggtggccg ctgcattcat gattgcgacg gtgctgcttg cgctcgtcac agaccatcga
    13321 cagtctcggg caaccgcgaa ctgagcatgc ttcctcaggc cctgccacat ccaggcctgg
    13381 ggaacagctg ccacacacgt tgcgcccaag caccaccaga gtgctcaggt tattggcatt
    13441 tcgggaaaaa tccgtgtaga cacggcgaac cggattgcca cgcacggctc gtgagacaag
    13501 gcccatgccc gcgaagcgca tcatctgtgt cggccacgcg gcgctcgacc ggatctatcg
    13561 catcagcgag tttcctaaag ccccgaccaa ggtgcgcgcg ctcgagcacg tcgaggtcgg
    13621 cggcggcatg gccgcgaacg cggcggtcgc gattgcccgc ctcggcggca agtcggagct
    13681 gtggagccgt gtcggcgacg acgccgccgg tcagacgatc cgcgcagggc tccgcgccga
    13741 gaaggtcgac atccgctacg tgcacgcctt cgaggacgcc cgctcgtcca cctcggcgat
    13801 catcgtcgac gaccacggcg agcgcctcat cgtcggccag cgcgacatca ccactccgac
    13861 gagcacgagc tggttgccgc tcgagcgcgt caccgaggcc gacgcggtgc tcggcgacgt
    13921 gcgctggctc gaggcgctga gagcggtctt cacgcgcgca cgcgacgccg gcgtcccgac
    13981 catcctcgac ggcgatgtcg gagcccgcga ggcgcttccc gagatcctgc cgctggtcga
    14041 ctatgcggtg ttctcggcaa cggcgctgcg cgaactcgag gcgggcggca cggacg
//
LOCUS       DAORNH010000093        14079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3262_length_14079_cov_4.740302, whole genome shotgun sequence.
ACCESSION   DAORNH010000093 DAORNH010000000
VERSION     DAORNH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_14079_cov_4.740302"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1136)
                     /locus_tag="PK264_11145"
     CDS             complement(114..1136)
                     /locus_tag="PK264_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HRD76477.1"
                     /translation="MVAMINPFDAGGYSLAEMTQAINILPNVYTRLGQMGLFRFEGVT
                     QRSVVIEQAEGVLNLLPTVPLGGPATVANRDTRSMRSFTVPWIPHDDVITPQDIQGVR
                     GFGVADAADPLATVMERKLTRMRVKHAQTREYMEVNALRGIVKDGAGTTLYNYFTEFG
                     LAQLETDFVLGTAGTQVQGKVRDVLRKVETELKGETMTGVLAMVSPEFFDKLIGHAKV
                     EEAYKYYSSTGAQPLREDTRRRFPFAGILFEEYNATVTLSTGATETLIPSGEGIAFPL
                     GTLDTFVTHGAPANLIETVNTVGLPIYARQIARPDGSAIEVKTEASILPVNKRPRLGV
                     RIFSSN"
     gene            complement(1231..1620)
                     /locus_tag="PK264_11150"
     CDS             complement(1231..1620)
                     /locus_tag="PK264_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HRD76478.1"
                     /translation="MTVLTMSSTLGDLLKYELNASYCREAVTLKAGTNYALGSVLGRI
                     TASGKYRLSPDAEVVGDEGAEVATAVLIEAVDATAGDRTGLVVARGPAIVSKAALVFD
                     ASVDDAAKTAVKHAELSSAGIVPRDTA"
     gene            complement(1638..2957)
                     /locus_tag="PK264_11155"
     CDS             complement(1638..2957)
                     /locus_tag="PK264_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRD76479.1"
                     /translation="MTRLNPLLTRLGGQPLAIAPRALDGLLAAGPMLDTRPAMLPARD
                     APPVASHSVTGPGIAVVPILGPLVTRGDWLTSLLGASDYGEIASAVEAALADPAARAV
                     LLELDSPGGEVGGLFDLVDRLVSLREAAQKPLWAVASESALSAAFAIASVADRLYVTR
                     TAEVGSIGVVAIHVDESVADVMAGLKWTLVHAGDRKIEGNAHEPLSDTALSAIQADVD
                     ALHADLVTLVARNRNMSPDAVRATEAAIYRGQRGIDAGLADKLGTVDFALVDLARALD
                     PPRLITGASQRARAHQPSRRKTAMTVEPDLNPAAEDAAVSETCAPDPETPEAPLSAPP
                     AGAPEATDTPNTDQTAKRLRAEYAEIAAIAAQGARLGVAIDAADAMAKGVAPHALRSS
                     ILDALAARAEASSVVAVAPSLAGSPASNGGESPIVRRARERASANRS"
     gene            complement(2965..4443)
                     /locus_tag="PK264_11160"
     CDS             complement(2965..4443)
                     /locus_tag="PK264_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRD76480.1"
                     /translation="MLESITRWRRRIGALVGGFEAGQGSRRLRHFQPSRAHLNTLIAA
                     AGADITARARWLVRNNGYAANAIESWAGNVVGDGIKPSSLIADADLKARVQRLWLDWT
                     DDSDAEGFTDFYGQQRRAAREVFIAGEVFFRFRPRRPDDGLTVPLQLQMIPSEMLPLS
                     RNEQVAGGNVIRQGIEFDRIGRRVAYHFLRRHPGDVTDPGLSGETVRVPASEVIHVID
                     PVDAGQLRGISRFAPGIVKLFLLDQYDDAELDRKKVAAMHALFITTPAPAEPFDVAES
                     DEGGERTMDLQPGQIVMLEPGEEVQTSAPADVGQTYEPFQYRTLLQVSAALGIPYAYL
                     SNDMLKANYSNSRLALLEFRRRIEAYQHSVMVWQICRRVWARWLDTAVMASAIALPDY
                     EQKRRIYLGCSWLPPKWDWVDPLKDARAEIEQIEAGLKSRTQALAERGYDADQVDAEI
                     AADRARERQLGLSFGSASSDPTLLIDAQEAASADNQANVAAD"
     gene            complement(4447..4659)
                     /locus_tag="PK264_11165"
     CDS             complement(4447..4659)
                     /locus_tag="PK264_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76481.1"
                     /translation="MTLEDMIARRDALLAARWRGVRTVEVEGRRITYASDAEMAAALG
                     DLERRIAEEQTGARRRIVRTTASKGL"
     gene            complement(4670..6646)
                     /locus_tag="PK264_11170"
     CDS             complement(4670..6646)
                     /locus_tag="PK264_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRD76482.1"
                     /translation="MLKGPARLAQATRKALTILAPPPTLTISEWADARRRLSSEASAE
                     PGRWRTERAIYQRGIMDAISDPAVESVVVMSSSQTGKTEVLLNTVAFHIDQDPAPVMV
                     VMPTERDAETLSKDRFSPMARDTPCLHGRISDPKSRDGSNKILHKKFPGGHLTIVGAN
                     APSGLASRPIRILLCDEVDRYPFSAGAEGDPVNLAKKRTVTFWNRKIVLVSTPTIRGA
                     SRIETAYAESDRRRFFVPCPECGEHQTLVWEQVRWDREADGAHRPETARYRCRHCGAH
                     WSDAERWAAVRRGEWRAEAPFDGIAGFHLNEVYSSWVRLEAMVRTFLSAKDHGDEAMK
                     TFVNTSLGETWVETGEAPDWQRLYDRRENWPAGTVPMGGLFLTAGADVQKDRIEVDVW
                     AWGRGLESWLIDHIIIEGGPEHAAAWSALDGLLGRNWPHASGVAMGLSRLAIDTGFEA
                     PSVYGWARRAGFAQVAPVKGVEGFNRASPVSGPTYVDATAGGKRLRRGARLWSVAVST
                     FKAETYRYLRLERPTDEERAGGARFPAGTIHLPAWADSEWCKQFVAEQLVTVKTRRGF
                     QRLEWQKLRERNEALDCRVYARAAAWIAGADRWSEEKWRDLERQVGSLDRRDTAAPET
                     TASDPGAPEIASAGLVRRAPARRGRRVFTPSYLS"
     gene            complement(6561..7130)
                     /locus_tag="PK264_11175"
     CDS             complement(6561..7130)
                     /locus_tag="PK264_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76483.1"
                     /translation="MATNTQPIAVIARLLDLTERRVQQLARDGIIPASVRAGPERGRY
                     DLVGTVRGYVRYLRELATRSQTGAADFGVERARLIKAKADLAEMDAQVRRADLLPAAQ
                     VEEAWIAVLARLRARLLVLPDRLAPLVHEESTIAGTRAQIRDAITEALAELASLPAIA
                     VDAEGTGAAGAGDTQGADDPGATTDPDDQ"
     gene            7277..7498
                     /locus_tag="PK264_11180"
     CDS             7277..7498
                     /locus_tag="PK264_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76484.1"
                     /translation="MTKSRNTASALDAFIAKKAEIDTMLERIKVLSDDHFDTNPDEIN
                     WGHVGTLAHYAELLKRITDAAFKEGEHAD"
     gene            7601..8146
                     /locus_tag="PK264_11185"
     CDS             7601..8146
                     /locus_tag="PK264_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3489 domain-containing protein"
                     /protein_id="HRD76485.1"
                     /translation="MTKLSDTQTIVLSAAAQRANMLALPLPKNLKGGAAQKVIASLLK
                     QGLLEEIDADTRIGEHIWRETGDGHGVTLAITEHGLAAIGIEPEASRDSAEPTQSGHA
                     AVKTPSKPNAREGSKQAQLIAMLQGADGATIAEITAAFGWQPHTVRGAIAGALKKKLG
                     LDVTSEKIEGRGRVYRLSREG"
     gene            8151..8396
                     /locus_tag="PK264_11190"
     CDS             8151..8396
                     /locus_tag="PK264_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76486.1"
                     /translation="MARITIHDRLVAALQHRGEAIIADARSTRYTVLTRTRRETGEQV
                     GFYFVGRAGALRAGRTIGESRSVGADFRAKLLGTTTR"
     gene            complement(8449..8670)
                     /locus_tag="PK264_11195"
     CDS             complement(8449..8670)
                     /locus_tag="PK264_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76487.1"
                     /translation="MMQSRRMSLIEALSNVAIGYGVAVLTQIAVFPLFGLQVSLSDNF
                     LIGALFTLVSVARSYAVRRMFERMRSWTA"
     gene            complement(8667..9989)
                     /locus_tag="PK264_11200"
     CDS             complement(8667..9989)
                     /locus_tag="PK264_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HRD76488.1"
                     /translation="MDVVETPIDKLVPYARNPRRNEEAVATVAASLAEFGWRQPIVVD
                     EDMVIVVGHTRYEAAKRLGMTSVPVHVAHGLTPAQLRAYRLMDNRSHQNASWDDELLK
                     LELADLKLDEFDLALTGFEDDELARLLAEAPVEGLVDEDEVPEPPATPVTRRGDLWIL
                     GDHRLLCGDSTSAEDVTRLMNGERAALFATDPPYLVDYDGTNHPTKKNASARAKKIAN
                     KDWSDDYIEQKHWDDSSQGPQFYEAFMQVAIDCAIKEGAAWYCWHASRRQAMLEACWS
                     KFDVLHHQQIIWAKSRPVLTRSIMLWAHEPCLFGWRSGNKPRVNREGFENWPTTVWSI
                     PSSEIETREHPTSKPVRVFTLPMELHTVPGEICYEPFSGSGSQLIAGERTGRRVFGLE
                     LSETFCDVIVNRWQAFTGKSARLEGENRSFDEVKTERVGTHDSDQDAA"
     gene            complement(10363..10509)
                     /locus_tag="PK264_11205"
     CDS             complement(10363..10509)
                     /locus_tag="PK264_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76489.1"
                     /translation="MMIAIMIMMIVIAMFANLWLWKKVKPIRARETSFNETFPGDTER
                     RDFG"
     gene            complement(10534..11718)
                     /locus_tag="PK264_11210"
     CDS             complement(10534..11718)
                     /locus_tag="PK264_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRD76490.1"
                     /translation="MENVPGTSSSRLGEYVETSVGGEKVRAFVPAPLPPVPALDVSQF
                     LSLYDRARGAVGRLDGVTTILPSTPLFLFMYVRKEALLSSQIEGTQSSLSDLLLFEND
                     EIPQVPIDDVTEVSNYVAAIEHGMRRMRGGFPLSLRLLREMHEILLKSGRGASKQPGE
                     FRRSQNWIGGTRPGNALFVPPPPNRLDECLAAFERFLHMDDPAMPPLIRAGLAHVQFE
                     TIHPFLDGNGRLGRLLITLMLCDAGALREPILYLSLFFKSRRDDYYRLLQEVRLAGGW
                     EAWMEFFLTGVAETAEQAADTARELMALFEADRLAIREHGRVAASVLRVHELLQRRPM
                     LTIQSAAKELKLSVPTVGKSLELMAKAGIVREVTGKQRGRLFAYTKYLALLDKGTEPL
                     PA"
     gene            complement(11827..12297)
                     /locus_tag="PK264_11215"
     CDS             complement(11827..12297)
                     /locus_tag="PK264_11215"
                     /inference="COORDINATES: protein motif:HMM:NF040389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6362 family protein"
                     /protein_id="HRD76491.1"
                     /translation="MAEWTRERVEERLIEAADVMKRLPPVRVQGYFSVWPAILPDFGD
                     LVGREPPRLRRPAPAPDAIDRMEAAMPWLRWLEPEDAKLVWARAEGTPWKPICWRFGI
                     SRATACRRWEYSLSLITWRLNGGTGKLKRSRAFLVDRVRSVNAPPWSAWSENRS"
     gene            complement(12290..12490)
                     /locus_tag="PK264_11220"
     CDS             complement(12290..12490)
                     /locus_tag="PK264_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76492.1"
                     /translation="MRWHPKGYGGERRSPEEVKREGWREQGLLVVSPADPRLTWPERE
                     LIRQLGEKLYGERLQATERRYG"
     gene            complement(12487..12744)
                     /locus_tag="PK264_11225"
     CDS             complement(12487..12744)
                     /locus_tag="PK264_11225"
                     /inference="COORDINATES: protein motif:HMM:NF040395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HRD76493.1"
                     /translation="MSGETMLRHAASVVAERRKVYGEPAAAMAVVARRWSITLGRPIT
                     PAEVVLCLIDLKLARLGHDPKHQDSILDVAGYAAVLQEIGR"
     gene            complement(12741..13211)
                     /locus_tag="PK264_11230"
     CDS             complement(12741..13211)
                     /locus_tag="PK264_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76494.1"
                     /translation="MHRAILTLDLGTTTGWAVRGADGHITSGTVSFRPSRYDGGGMRY
                     LRFRAWLDSIVADAGGLTAIYFEEVRRHVGTDAAHLYGGFLATLTAWCEQQAISYQGV
                     PVGTVKRHIAAKGNADKAAVMTAVRARGFSPTDDNEADAIAILLWAIETEGGVR"
     gene            complement(13370..>14079)
                     /locus_tag="PK264_11235"
     CDS             complement(13370..>14079)
                     /locus_tag="PK264_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76495.1"
                     /translation="GSYETWSRIMGGVLEVAGIEGFLANLDEMLAAADGEGAIWRSFV
                     GAWWDRFGTAEVGTGDLYEVALTCEPPLPLGTGGDRSQRTRLGKALARMRDRVFDIDG
                     RKMRVRTLGVSHQAKRWQLMIEGERGERFPQGVEVQGGERAGPEGNVENQRSPAQPLE
                     TKGSRERGEHGERFSILTHARGCTRAIEDREQRSPCSPPSQSVGSSNAYDGEPGGERP
                     PSRSPDGAAPDWLKEVL"
BASE COUNT         2356 a         4700 c         4674 g         2349 t
ORIGIN      
        1 gacggcgcgg atcgccacgc cggatccggc gcctgccacc cgccagatcg catcgtgcgc
       61 aagattggga tcggcgaaga ggtcgtcgat cgcctctgcg aagatgccca tgctcagttg
      121 ctggagaaga tgcgcacgcc gagacgcgga cgcttgttga ccggtaggat cgaggcctcg
      181 gtcttgacct cgatggcgct gccgtcgggg cgggcgatct ggcgcgcgta gatcggcagg
      241 ccgaccgtgt tgaccgtctc gatcaagttg gccggcgcgc cgtgggtgac gaaggtatcg
      301 agcgtgccga gcgggaaggc gatgccctcg ccggagggga tcagggtttc cgttgcgccg
      361 gtcgagagcg tgacggtggc gttgtactcc tcgaacagga tgccggcgaa gggaaagcgc
      421 cggcgggtgt cctcgcgcag cggctgcgcc ccggtcgagg aatagtactt gtaggcctcc
      481 tcgaccttgg cgtgaccgat cagcttgtcg aagaactcgg ggctcaccat cgccagcaca
      541 ccggtcatgg tctccccctt gagctcggtc tcgaccttgc ggagcacgtc gcgcaccttg
      601 ccctggacct gcgtgccggc ggtgccgagc acgaagtcgg tctccagctg cgccagaccg
      661 aactcggtga agtagttgta gagcgtggtg ccggcgccgt ccttgacgat gccgcggagc
      721 gcattgacct ccatgtactc gcgggtctgg gcgtgcttca cccgcatgcg ggtgagcttg
      781 cgctccatga cggtggcgag cgggtcggcg gcatcggcca cgccgaagcc gcgcacgccc
      841 tggatgtcct ggggcgtgat cacgtcgtcg tggggaatcc acggcaccgt gaaggagcgc
      901 atggagcgcg tgtcgcggtt ggcgacggtg gcgggaccgc cgagcggcac ggtcggcagg
      961 aggttcagca cgccttccgc ctgctcgatg acgacggagc gctgtgtaac gccctcgaag
     1021 cggaacaggc ccatctgccc gagccgggtg tagacgttgg gcaggatgtt gatggcctgg
     1081 gtcatctcgg cgagcgagta gccgcccgcg tcgaacgggt tgatcatggc gaccatgatg
     1141 tcgggtctcc ttgggatgga acgggcatga aaaaggcccc gaaggcggac gcctcggagc
     1201 cggtgacgga ttggatccga cgagcgcgga tcaggcggtg tcgcgtggta cgatgccggc
     1261 agagctcagc tcggcgtgct tgacggccgt cttggccgcg tcatcgacgg aggcgtcgaa
     1321 gacgagcgcc gccttggaga cgatcgccgg accgcgggca accacgaggc cggtcctgtc
     1381 gccggccgtc gcgtcgaccg cctcgatcag gacggccgtt gcgacctccg caccctcgtc
     1441 tccgacgacc tcggcgtccg gcgacaggcg gtacttgccc gaggcggtga tccggccgag
     1501 gacggatccg agcgcgtagt tcgtgccagc cttgagggtc acagcctcgc ggcagtagct
     1561 cgcattgagc tcgtacttga gcaggtcgcc aagggtcgac gacatggtga gaacagtcat
     1621 gatgatcctc cttgtcgtca gctgcggttg gccgaggcgc gctcacgcgc acgacgcacg
     1681 atggggcttt cgccgccgtt cgaggccggc gagccggcca gtgacggcgc cacggcgaca
     1741 acagagctcg cctcggcgcg tgccgcgagg gcgtcgagga tggagcttcg cagcgcatgc
     1801 ggcgccactc ccttcgccat ggcgtcggcg gcgtcgatgg cgacgcccag ccgggcccct
     1861 tgggcggcga tggcggcgat ctccgcatac tcggcgcgca gccgcttggc cgtttgatcg
     1921 gtattgggcg tgtcggttgc ttccggtgcg ccagcaggcg gtgccgacag cggagcctcg
     1981 ggtgtttccg gatcgggtgc gcaggtctcg ctgacggcgg cgtcttcggc agccgggttg
     2041 aggtcgggtt ctactgtcat tgcggtcttt ctccttgaag gttgatgagc gcgggcgcgt
     2101 tgcgatgcgc ccgtgatgag gcgtggcggg tccagcgccc gggcgagatc cacaagagcg
     2161 aaatcgacag tgccgagctt gtcggccagg ccggcgtcta tgccgcgctg accccgatag
     2221 atcgcggctt cggtggcgcg cacggcgtcg ggactcatgt tccgattgcg cgccaccagg
     2281 gtgacgaggt cggcatggag cgcgtcgaca tccgcctgga tcgccgacaa cgccgtatcc
     2341 gagagcggct cgtgggcatt gccctcgatc ttgcgatcgc ccgcgtgaac gagcgtccat
     2401 ttgaggccgg ccatgacgtc ggcgacgctc tcgtcgacat ggatggcgac gacgccgatg
     2461 gatccgacct ccgccgtccg ggtgacgtag aggcggtccg ccacgctggc gatggcaaag
     2521 gcagccgaca gcgcgctttc gctcgcgacg gcccagagcg gtttctgcgc ggcttcgcgc
     2581 agggacacga ggcgatcgac caggtcgaac agaccgccga cttcaccgcc tggcgagtcc
     2641 agctccagca acacggcccg cgcggctgga tcggccagcg cggcttccac ggcggaggcg
     2701 atctcgccat agtcgctggc gcccagaaga ctggtgagcc agtcgccacg cgtcaccaac
     2761 ggtccgagga tcggcaccac ggcgatgccg ggaccggtaa cggaatggct cgccaccggc
     2821 ggcgcatcgc gggccggaag catggccggc cgtgtatcga gcattgggcc ggcggcgagc
     2881 aggccgtcga gcgcccgtgg ggcgatcgcc aagggctggc cgccgagccg ggtgagcagc
     2941 ggattcaatc gcgtcatgga aacctcagtc ggcggcgacg ttcgcctggt tgtcggccga
     3001 tgctgcctct tgagcatcga tcagcagcgt cgggtcggat gaggcgctgc cgaaggagag
     3061 gccaagctga cgctctcgcg cacggtccgc agcaatctcg gcatcgacct gatcggcgtc
     3121 atagccgcgc tcggcgagcg cctgcgtccg gctcttcagc cccgcctcga tctgttcgat
     3181 ctcggcgcgc gcgtccttca acggatcgac ccagtcccat ttgggcggca accaggaaca
     3241 gccgagataa atgcgcctct tctgttcgta atcgggcaag gcaatcgcac tcgccatgac
     3301 cgccgtatcg agccagcgcg cccagacccg tcgacagatc tgccagacca tgaccgaatg
     3361 ctgataggcc tcgatgcggc ggcgaaactc gaggagcgcg agccgcgagt tcgagtagtt
     3421 cgccttcagc atgtcgttcg acagatacgc gtacggaatg ccgagcgccg ccgagacctg
     3481 cagcagcgtg cggtactgga acggttcgta ggtctggccg acatcggcag gcgccgaggt
     3541 ctgcacttcc tcgcccggct ccagcatgac gatctggccg ggctgcagat ccatcgtccg
     3601 ctcgccgcct tcgtcgctct cggcgacgtc gaagggctcc gccggcgccg gcgtggtgat
     3661 gaagagcgcg tgcatcgccg cgaccttctt ccggtcgagc tccgcatcgt cgtactgatc
     3721 gagcaggaac agcttcacga tgcccggcgc aaagcgggaa atcccgcgca actgccccgc
     3781 atcgaccgga tcgatcacgt ggatgacctc ggacgccggc acccgcaccg tctcgccgga
     3841 gaggcccgga tcggtcacgt cgccgggatg gcggcgcagg aagtggtagg ccacacgcct
     3901 gccgatccgg tcgaattcga tgccctggcg aatgacattg ccgcccgcca cctgctcatt
     3961 gcgcgagagt ggcagcatct cggaggggat catctgcagc tgcagcggca ccgtgagccc
     4021 gtcgtcgggc cggcgcggac ggaagcggaa gaacacctcg ccggcaatga atacctcgcg
     4081 cgcggcgcgc cgctgctgac catagaaatc ggtaaagcct tcggcgtcgc tgtcgtcggt
     4141 ccagtcgagc cagagtcgct gcacgcgcgc cttgagatcg gcatctgcga tcagggatga
     4201 cggcttgatg ccgtcgccca ccacattgcc ggcccagctc tcgatggcgt tcgccgcata
     4261 gccgttgttg cgcacgagcc agcgggcgcg tgcggtgatg tcggcgccgg cagcggcgat
     4321 cagcgtgttg agatgcgccc ggctcggctg gaagtgccgc agccttcggc ttccctgtcc
     4381 cgcttcaaag ccgcccacca gagcgccgat gcggcggcgc caccgtgtga tcgattccag
     4441 cacgggtcag agccccttgc ttgccgtcgt gcgaacgatg cgacggcgcg cgccggtttg
     4501 ctcctcggcg atccgccgtt cgaggtcacc gagggcggcc gccatttcgg catcgctcgc
     4561 ataggtgatg cggcgcccct cgacctcgac ggtgcgcacg ccgcgccatc gggcggcgag
     4621 cagcgcatcg cggcgcgcga tcatgtcctc aagcgtcatg gtcctggtct caactcagat
     4681 agctcggcgt gaacacccgt cggccgcgcc gggctggtgc tcgtcgcacc agacccgcgg
     4741 aggcgatctc tggcgcgccg gggtcagagg ccgtggtctc tggcgccgct gtgtcgcgtc
     4801 gatcgagcga gccgacctgg cgttcgagat cacgccattt ctcctcgctc cagcggtcgg
     4861 cgccggcgat ccaggcggcg gctcgcgcat agacccggca atccagcgcc tcgttgcgtt
     4921 cgcgcagctt ctgccattcg agccgctgaa acccgcgcct ggtcttcacc gtcaccagct
     4981 gctcggcgac gaattgcttg caccactcgc tgtcggccca agccggcagg tggatggtcc
     5041 ctgcgggaaa gcgcgcgcct ccggcacgct cctcgtcggt cggccgttcg agccggaggt
     5101 agcgataggt ctcggctttg aaagtcgaga ccgcgaccga ccacagccgc gcgccgcgcc
     5161 gcagtcgctt gccgccggcg gtcgcatcga cataggtcgg tccggagacc gggctcgccc
     5221 gattgaaacc ctcgacaccc ttgaccggcg ccacctgcgc gaagccggcg cgccgggccc
     5281 agccgtagac cgacggcgcc tcgaagccgg tgtcgatcgc gaggcgcgac aggcccatcg
     5341 ccaccccaga ggcgtgcggc cagttgcggc ccaagagacc gtccaacgct gaccaggcgg
     5401 cggcatgttc ggggccgccc tcgatgatga tgtggtcgat gagccagctc tccagcccgc
     5461 gaccccatgc ccagacatcg acctcgatgc gatccttctg gacgtcggcg ccggcagtga
     5521 gaaacaggcc gcccatcggc accgtgccgg ccggccagtt ctccctgcga tcgtagagcc
     5581 gctgccagtc cggcgcttcg cccgtctcga cccatgtctc gcccagcgat gtattgacga
     5641 aggtcttcat cgcctcgtcg ccgtgatcct tcgccgacag aaaggtgcgc accatggctt
     5701 ccaagcggac ccaggaggaa tagacctcgt tcaggtgaaa gccggcgatg ccgtcgaacg
     5761 gggcctcggc ccgccattcg cccctgcgca cggcggccca gcgttcggcg tcgctccaat
     5821 gcgcgccgca atggcggcat cggtatcgcg cggtctccgg ccggtgggcg ccgtccgcct
     5881 cgcggtccca gcgaacctgc tcccagacca gcgtctgatg ctcgccgcat tccgggcacg
     5941 gcacgaagaa gcggcgcctg tcgctttctg cataggcggt ctcgatccgg ctcgcgccac
     6001 ggatggtcgg cgtcgagacc aggacgatct tgcggttcca gaaggtgacg gtgcgcttct
     6061 tcgccagatt gaccggatcg ccctcggcgc cggcgctgaa cggatagcgg tcgacctcgt
     6121 cgcacaggag gatgcggatc ggccggctgg ccaggcccga cggcgcattc gcgccgacga
     6181 tggtcagatg cccgccggga aacttcttgt gcaggatctt gttcgaaccg tcccgcgact
     6241 tcggatccga gatgcgccca tgcagacagg gcgtatcgcg cgccatgggc gagaaacggt
     6301 ccttcgacaa ggtctcggca tcccgttccg tcggcatcac caccatcacc ggcgccgggt
     6361 cctggtcgat gtggaatgcg acggtgttga gcagcacctc cgtcttgccg gtctggctcg
     6421 acgacatcac gacgacgctt tcgaccgccg gatcggagat cgcgtccatg atgccgcgct
     6481 ggtagatggc ccgctcggtg cgccagcggc cgggctcggc gctggcttcg gaactcaggc
     6541 gacgcctggc gtcggcccac tcactgatcg tcagggtcgg tggtggcgcc aggatcgtca
     6601 gcgccttgcg tgtcgcctgc gccagccgcg ccggtccctt cagcatcaac ggcgatggcc
     6661 gggaggctgg cgagttccgc gagcgcttcg gtgatcgcgt cgcggatctg cgcgcgcgtg
     6721 ccggcaatgg tggactcctc gtggaccagc ggcgccagcc tgtcgggcag gaccagcaaa
     6781 cgtgcgcgca ggcgggccag cacggcgatc cacgcctcct cgacctgagc ggccggcaag
     6841 aggtcggcgc gccggacttg cgcgtccatt tcggcgagat cggccttcgc cttgatcaac
     6901 cgggcgcgct cgacaccgaa atccgcggcg cccgtctgcg accgcgtcgc cagttcgcgc
     6961 aggtagcgca catagccgcg caccgtgccg acgagatcgt agcgcccgcg ttcggggccg
     7021 gcccggaccg acgccgggat gatcccgtcg cgcgccagct gctggacccg cctttcggtc
     7081 aggtccagga gccgagcgat caccgcgatc ggttgagtat tggtcgccat aaacggggcc
     7141 gctcccgggc aagatcaggt catgtcggga gccccgccat cactgcagaa aaagcaatga
     7201 aatgatgcac ttatcgactt gatgagggtg ccgatcagag cctgtatggg gtcaccatca
     7261 agcgctggag accgccatga ccaagtcccg aaacaccgct tccgccctcg acgctttcat
     7321 cgccaagaag gcggaaatcg acacgatgct ggagcgcatc aaggtgctga gcgacgacca
     7381 tttcgacacc aaccccgacg agatcaactg gggccatgtc ggaacgctcg cgcactacgc
     7441 cgagctcctg aagcgcatca ccgacgcagc cttcaaggag ggcgagcacg ccgattaggc
     7501 gcgccgcttc ccgccttcgc cccgatgggc tcgccctcgg ggctcggggc agtagaaggt
     7561 ccgcgatggt cgcgcgcctc tcctgaagaa ggattgcccc atgaccaaac tctccgacac
     7621 acagacgatc gtcctcagcg ccgccgcgca gcgcgcgaac atgctggcgc taccgctacc
     7681 gaagaacctc aagggcggcg cagcgcagaa agtgatcgct tcgctcctca agcagggcct
     7741 gctcgaagag atcgatgccg acacgcgcat cggcgaacac atctggcgcg agaccggcga
     7801 cggccacggc gtcacactcg cgatcaccga gcacgggctc gccgccatcg gcatcgagcc
     7861 ggaggcctcg cgtgactctg cggagccgac gcaaagcggt cacgctgccg tcaagacgcc
     7921 atcgaagcca aatgcccgcg aaggcagcaa gcaggcccag ctgatcgcca tgctgcaggg
     7981 cgccgatgga gcaaccatcg ccgaaatcac tgccgcattc gggtggcagc cgcacaccgt
     8041 gcgcggcgcc atcgccgggg cgctcaagaa gaagctcggg ctcgacgtga cctccgagaa
     8101 gatcgaggga cgcggtcggg tctaccgcct cagccgggag ggttgaggcc atggcgagga
     8161 tcaccatcca cgaccgtctc gtcgccgccc tgcagcaccg aggcgaagcg atcatcgctg
     8221 atgcacgctc gacccgctac acggtcctca cgcgaacgcg ccgggaaacc ggcgaacagg
     8281 tcggcttcta ttttgtcggc cgtgccggcg cgctccgggc cggccgcacc attggcgaga
     8341 gccggtcggt gggcgccgac ttccgggcga agctgctcgg aacgaccacc cgctgacacg
     8401 ccatcatcac tgagccgccg ctgcccgcca tgggcggcgg cgttttgctt atgcagtcca
     8461 agagcgcatt ctctcgaaca tgcgccgcac ggcatagctg cgcgccacgg ataccagcgt
     8521 gaatagagcg ccgatcaaaa aattgtcgct cagggacacc tgcagaccga acagcgggaa
     8581 gactgcgatc tgggtcagca cggccacgcc gtaaccgatc gcgacattgc tcaacgcctc
     8641 gatcagggac attcggcgcg actgcatcat gcggcgtcct gatcgctgtc atgagtgccg
     8701 acgcgctcgg tcttcacctc gtcgaagctg cgattctcgc cctccagcct tgccgatttc
     8761 ccggtgaagg cctgccagcg gttgacgatc acgtcacaaa aggtctcgga gagctcaagc
     8821 ccgaagacgc gtctccccgt ccgctcgcct gcgatgagtt gcgagcccga gccggagaac
     8881 ggctcgtagc agatttcgcc tggcaccgtg tgcagttcca tcggcagcgt gaatacgcgc
     8941 accggtttcg aggtcgggtg ctcccgcgtc tcgatctcgc tcgacgggat ggaccacacc
     9001 gtcgtcggcc agttctcgaa gccttcgcgg ttgacgcgcg gcttgttgcc cgagcgccag
     9061 ccgaacaggc agggctcgtg cgcccacagc atgatcgagc gcgtgagcac cggacggctc
     9121 ttggcccaga tgatctgctg gtgatgcaga acgtcgaatt tggaccagca ggcttccagc
     9181 atcgcctggc gccgcgaggc atgccagcaa taccaggccg cgccctcctt gatggcgcag
     9241 tcgatggcga cctgcatgaa ggcctcgtag aactgcggcc cttgggatga atcgtcccag
     9301 tgcttctgct cgatgtagtc gtcggaccag tccttgttcg cgatcttctt ggcgcgagcg
     9361 gacgcgttct tcttcgtcgg atggttggtg ccgtcatagt cgacgagata gggcggatcg
     9421 gtcgcgaaca gcgcggcgcg ctcgccgttc atcaggcggg tgacgtcctc agccgaggtc
     9481 gagtctccgc agagcaggcg gtggtccccg aggatccaga gatcgccgcg gcgggtgacg
     9541 ggcgtggcgg gcggctccgg aacctcgtct tcgtccacca gcccctcgac gggcgcctcg
     9601 gccaggagac gggccagttc gtcgtcctcg aaaccggtca gcgccaggtc gaactcgtcg
     9661 agcttcagat cggccagttc gagcttgagc agctcgtcgt cccagctcgc attctgatgc
     9721 gagcggttgt ccatcagccg gtaggcgcgc agctgcgccg gcgtcaggcc atgcgcgaca
     9781 tgcaccggca cactcgtcat gccgaggcgc ttggccgcct cgtaccgggt gtggccgacg
     9841 acgatcacca tgtcctcgtc gacgacgatc ggctgtcgcc agccgaactc ggccagcgag
     9901 gcggcgaccg tggcgacagc ctcctcgttg cggcgcgggt tgcgcgcata gggcacaagc
     9961 ttgtcgatcg gcgtttcgac gacgtccatg gtcggtccgg tgcgatgggt gaaggatctg
    10021 atccggcggt cgcgaagccc gccgatgcgc tgtgccggcg aaaccagtgc cgcttcgcct
    10081 atgcgctcga aacgaaacgc ccccgaccgg cggcttcgcc agcgccgggg tcagcagacg
    10141 cccaagtgct tggattcacg aggccaagga tgcgggcgaa acgaaatggc ctatttcggc
    10201 gccgtcactg ggcaagcgtc gcgccattgc cgccagcata cgaaattggc caggaaggac
    10261 ccgtgatatc agcggcttag gaaatttggg cgtaaatggg tgtcgatttg ggtgccgggg
    10321 cggagtggct ttgcgcgcgc ctcgcccgag cgtgatcgaa acctaaccaa aatcgcgccg
    10381 ctctgtatcg ccgggaaatg tctcattgaa acttgtctca cgcgcccgaa taggcttgac
    10441 ctttttccaa agccagaggt tggcgaacat ggcgatgacg atcatcatga tcatgatcgc
    10501 gatcatcatc cgctcgcgcg atttgcggac ggctcaggct ggtagcggct cggtgccttt
    10561 gtccagcagc gcaaggtatt tcgtgtaggc gaacagccga ccgcgctgct tcccggtcac
    10621 ctcccgaacg atgcctgcct ttgccatcag ctcgagcgac ttgccgacgg tcgggaccga
    10681 gagcttcagc tccttggctg cggactggat cgtcagcatg ggacgccgtt gcagcagctc
    10741 gtgaaccctc agcacggagg ccgcgacgcg tccgtgttcg cgaatggcga gccggtcggc
    10801 ctcgaacaac gccatcaact cgcgcgctgt gtcggcagcc tgttccgccg tctcggcgac
    10861 gcctgtcagg aagaactcca tccacgcttc ccagcctccc gccagacgca cttcctggag
    10921 caggcggtaa tagtcgtctc gacggctctt gaagaaaaga ctgaggtaga ggatcggctc
    10981 gcgcagcgcg ccagcgtcgc acagcatcag agtgatcaag agacgtccaa ggcgtccgtt
    11041 tccatcgagg aagggatgga tcgtttcgaa ctgcacatgg gcaagccccg cccgaatgag
    11101 tggcggcatc gccgggtcgt ccatatgcag aaaccgttcg aatgcggcga ggcattcatc
    11161 gagccgattg ggcggtggcg gcacgaacag ggcattgcca ggcctcgtcc ccccgatcca
    11221 gttctgcgac cggcgaaact cgcccggctg cttgctggca ccgcgaccgg acttgagcag
    11281 gatctcgtgc atctcccgca ggagacgaag cgatagcgga aagccgccac gcatgcgccg
    11341 catgccatgc tcgatcgcgg cgacatagtt ggacacctcc gtcacgtcat cgatgggaac
    11401 ctgggggatt tcgtcgttct cgaacaggag cagatcggac agggaggatt gcgttccctc
    11461 gatctgcgag gacaggagcg cttccttgcg cacatacatg aagaggaaca gcggcgtgga
    11521 aggcaggatg gtggtgacgc cgtcgagccg cccgacggcg ccgcgggccc ggtcgtagag
    11581 cgacaggaac tggctgacat ccagcgctgg aacggggggc aagggcgcgg ggacgaaggc
    11641 ccgcaccttc tcaccgccga ccgatgtttc gacgtattct ccgagcctgc tgctcgatgt
    11701 tcctggcacg ttctccatat cctttaatag cacacgtcca ttaataaagg aaagtggctt
    11761 ttcgtttact taacggacgc gccaaggaaa ggatcgcgtg tgcgcggcct gatcggggat
    11821 tgggcctcat gagcgattct ccgaccaggc gctccatggc ggcgcgttga ccgaacgaac
    11881 gcggtctacc aggaacgcgc gcgagcgctt gagcttccca gtgccgccat tcagccgcca
    11941 cgtgatcagg ctgaggctgt attcccagcg ccggcaggcc gtcgcccgcg agatcccaaa
    12001 gcgccagcag atcggcttcc acggcgtccc ctcggcgcgg gcccagacga gtttcgcgtc
    12061 ctcaggttcg agccagcgaa gccacggcat ggccgcctcc atccggtcga tcgcgtccgg
    12121 tgccggggcg gggcgccgaa gccgcggcgg ctcgcgccca accagatcgc caaaatcagg
    12181 caggattgcc ggccacacgg agaagtaccc ctgcacgcgc acgggcggca gacgcttcat
    12241 cacgtcggcg gcctcgatca ggcgctcctc gacccgctcg cgtgtccact cagccatacc
    12301 gtcgctccgt cgcctgcagc ctctctccgt agagcttctc gccaagctgg cggatgagtt
    12361 cgcgttcggg ccatgtgagg cgcgggtcgg ctggactgac gaccagcaat ccttgctcac
    12421 gccagccctc gcgcttcacc tcctcgggcg accggcgttc gccgccatag cctttcggat
    12481 gccacctcat cgcccgatct cctgcagcac cgctgcatag ccagcgacgt cgaggatcga
    12541 gtcctgatgc ttgggatcat gcccgagccg cgctagcttc agatcgatga ggcagagcac
    12601 gacctccgcc ggcgtgatgg ggcggccgag cgtgatcgac cagcgtctgg cgaccacggc
    12661 catcgcggcg gcgggttctc catagacctt gcggcgctcg gcgacgaccg atgcggcatg
    12721 cctcagcatc gtctccccgc tcatcgcacg cctccctcgg tctcgatggc ccagagcagg
    12781 atggcgattg catccgcttc attgtcatcc gtcggggaaa agccccgggc gcgaacggcg
    12841 gtcatgacgg cagccttgtc ggcgttgccc ttcgccgcga tgtgccgctt gacggtgccg
    12901 accggcacgc cctgatagga aatcgcctgc tgctcgcacc aggcggtcag cgtcgccagg
    12961 aagccgccat agagatgcgc ggcgtcggtt ccgacgtgac gccgcacttc ctcgaagtag
    13021 attgcggtga ggcccccggc atcggccacg atcgagtcga gccatgctcg gaagcgcaga
    13081 tagcgcatcc cgccgccatc gtagcgactg ggccggaacg aaacggtccc gctggtgata
    13141 tggccatccg cgccgcggac cgcccatcct gtcgtcgtcc cgagatcgag cgtcaggatg
    13201 gcgcgatgca catctgtcgg tctcggtttg aattgggtcg cccccgcgtc aacgggtgcg
    13261 gtttcggtca tcgcgtgcat gatgctctcc cagaagcgtg agaaatggtc ggtccagatt
    13321 tgggaggcca cttgcggcgg accggtcgcc gcttgtcgga gcgcgctcat cagagcacct
    13381 ccttcagcca atcgggcgca gcgccgtccg gggaacgtga gggaggacgt tccccgccag
    13441 gttccccatc ataagcgttt gaagaaccga cgctttggga aggtggggaa catggggaac
    13501 gttgttcccg atcctctatc gcgcgggtgc agccgcgcgc atgcgttagt attgaaaaac
    13561 gttccccatg ttccccacgt tccctggagc cttttgtttc aaggggttgg gccggggaac
    13621 gttgattttc gacgttcccc tccgggcccg cacgttcccc gccttgaacc tcgacacctt
    13681 gcggaaaacg ttccccacgt tccccttcga tcatgagctg ccagcgctta gcctgatgag
    13741 agacacccag ggtgcgcacg cgcatcttgc ggccgtcgat gtcgaagacc cgatcacgca
    13801 tgcgggcgag cgccttgccg agccgcgtgc gctgcgaacg atcaccgccc gtcccgagcg
    13861 gcaacggcgg ctcgcaggtc agcgccacct catagaggtc gccggtgccg acctctgcgg
    13921 tcccgaagcg atcccaccag gcgccgacga agctgcgcca gatcgcgccc tcgccatcgg
    13981 cggcggccag catctcatcg agattggcga gaaacccttc gatgccggcg acctcgagca
    14041 cgccgcccat gatgcgcgac caggtctcgt aactcccga
//
LOCUS       DAORNH010000094        14079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3263_length_14079_cov_2.769110, whole genome shotgun sequence.
ACCESSION   DAORNH010000094 DAORNH010000000
VERSION     DAORNH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_14079_cov_2.769110"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2406
                     /locus_tag="PK264_11240"
     CDS             <1..2406
                     /locus_tag="PK264_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD76496.1"
                     /translation="SFELGILVSVIAVVGILPYISLQLKAISASFSILVQYPEVRMPA
                     STAASILSDTELYVTLAMATFAILFGTRHIDASERHEGMVAAIAFESGVKLIAFLAVG
                     LFVTYGVFNGFGDIFGRAASDARLQRLFTIDGMAGHVSWLTVLLLAMAAIVVLPRQFQ
                     VLVVENVNEGHLRQAMWLFPLYLLLINIFVLPIAFAGLLRLPATGVDADTFVLTIAMQ
                     ERAPMLALLAFIGGLSAATGMIIVETVALATMVSNDLVMPLLLRIGRLHLSERRDLTG
                     ILLAIRRGTILLVLLLGYVFASTVGGSYALVTIGLVSFVAALQFAPPILAGLYWKGAT
                     KAGALAALSGGFAVWLYTLLLPSFAKSGWLPSAFVDAGLFGSVLLKPYALLGLEGLDP
                     ISHATIWTMLVNVGLLIGVSLFTSQSMIERSQAVQFADVFKSASSATRVWRGRVTKAE
                     LFALLGRFVGDARSDTALARYEQEIGRTLDRTAAADAALVAYVERQLAGAIGAASARI
                     MMASVLREEMQDIDAVTRMLDEASQLVLYSRRLEEKSQELEAASAELRSANERLKELD
                     KLKDDFMATVSHEIRTPLTSIRSFSEILRDNPDLDNEQRQEFVTIIVQESERLSRLIN
                     DILDLAKMEAGTTDWQIELQDAKPIFEQALAATSGLFAKAPGIRLGIEIAPDLPKVSV
                     DRDRLIQVVVNLISNAVKFSDKGKGEIRFKAWRHDNTLRVDVADNGIGIAPADQARVF
                     ERFQQAGNILTDKPEGTGLGLPITRQILQRFGGDIWLQSAVGKGSVFSFSIPLAAAGD
                     ARG"
     gene            2576..4267
                     /locus_tag="PK264_11245"
     CDS             2576..4267
                     /locus_tag="PK264_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76497.1"
                     /translation="MATEATLHNPEARSRPMTGEERKVILASSLGTVFEWYDFYLYGA
                     LAVIIGAQFFSAFDVTTRNVFALLAFAAGFLVRPFGALFFGRLGDLIGRKATFLITIL
                     LMGTTTFMVGLLPSYDQIGWVAPVVLIALRMGQGLALGGEYGGAAVYVAEHAPQGRRG
                     FYTSWIQTTATLGLLLSLVVILSIRLYMGEEGFSKGTGYPIAGWRIPFLASIILLAIS
                     VWIRMQMHESPAFKKMKEEGSLSKAPLSEAFGQWKNLKVVIFALLGLVAGQAVVWYTG
                     QFYALFFLQSILKVDLFTANVLIAWSLILGTGGFIVFGTLSDKIGRKPVILAGCLIAA
                     LTYFPLFKMMTATANPKLAEAIEKVKVQVTADPAKCGSVFDPVGIRSFSEPCDVARVS
                     LARAAITYNLVPGPAGSPAKVTFNGKDVPIEAAIPKNISGFTAAAIAAGYPKAGDTSI
                     VKTTSVIPSGAQAWTIIGILTLLVVYVTMVYGPIAAALVEFFPTRIRYTAMSLPYHIG
                     NGWFGGFLPPTSFAMVAATGDIYYGLWYPVVVALATFVIGLFFVPETYKRDIFKG"
     gene            4400..5140
                     /locus_tag="PK264_11250"
     CDS             4400..5140
                     /locus_tag="PK264_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRD76498.1"
                     /translation="MLMALLADVHANREAFEACLVDARRSGAERFILLGDLVGYGPDP
                     VWAVETAARLLDDGALAVQGNHDAAASGAATSLNPVARAAIRWTAEQLGPAHKQFLAD
                     LPLDLNEDDRLYVHASASSPEDWIYVLSPREAFASFRATAQRITFCGHTHLPALFNES
                     ASSLPQQHTPADGRPVPLLVQRRWLAVLGAVGQPRDHNPAACYALFDDETRRLTYIRV
                     PYDADVTARKVRAAGLPDALAVRLVSGS"
     gene            5144..6562
                     /locus_tag="PK264_11255"
     CDS             5144..6562
                     /locus_tag="PK264_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRD76499.1"
                     /translation="MAKASLSIGMVLDGFRLEERLHRGGMATIWRVTRPDIDDPIVMK
                     VPFLDHEGDLSLLVGFEVEQMIMAELAGPHVPRFIANGDFTVQPYIVMERLPGNNLHK
                     MLFDTPRLEIEDAISKGTAIAEALGDLHNQHVLHLDLKPANVLFRPSGEAVLIDFGLS
                     RHLLLPDLLEEQFHRPTGTTEYMAPEQLARVRSDKRSDLFALGAIIYQMATGELPFGA
                     PGRIKQVRQRVWRDPVPPRALRPELPAPLQEVIIKCLEPLPDKRYRDAEELVFDLRHL
                     DLVELTERAGRQARADARTAFGRWMRAKSTLREIQSTATKPPPRTPIILVAVDLRPRF
                     VELRNSLLEAAVSVLANMPGARLACLNVMETSLISIDENVDAAGDNIHVQRMAELRQW
                     AAPLQLPRGKITYHLVEARNIAGAILDFARSNKVDHLVIGAPTAGGAAASRLTAQITA
                     ESPCSVSVVRASADRADLVPVR"
     gene            6701..7657
                     /locus_tag="PK264_11260"
     CDS             6701..7657
                     /locus_tag="PK264_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76500.1"
                     /translation="MGWELSGRRLIVTGGAGFIGSHLSERLLAKGAEVLSVDNYFTGR
                     RANIAHLLQNPSFEAMRHDITFPLYVEADGILNLACPASPVHYQHDPVQTTKVSVHGA
                     INMLGLAKRLRCRILQASTSEVYGDPTVHPQPEEYWGNVNPIGPRSCYDEGKRCAETL
                     FFDYRRQHNLPIKIARIFNTYGPRMQPNDGRVVSNFIVQALQGEPITIYGDGAQTRSF
                     CYVDDLVDGLVRLLQTGEDMTGPINLGNPSEFTIRALAEKVIEMTGARSKLEFRPLPH
                     DDPRQRQPDITKAKSILGWRPTVELEDGLKETIGYFRRLLAQ"
     gene            7654..8844
                     /locus_tag="PK264_11265"
     CDS             7654..8844
                     /locus_tag="PK264_11265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD76501.1"
                     /translation="MSGAPSQPEARTAARPLHIVQVSFDASLLDGGEASDSIARQKLY
                     SRLLAERRPGSRMTIVVLNAPATAVACSWDNLHVVPISGRWASLRRLPAALRALDREA
                     AIGVIAAQSPFEDAWAALMFARRRIPVVVQVHFDVLAAEALPRGSMIRRLAGKARQRL
                     ALRSLRRFASVRVVAAAMKRRLEAAGARDVRAIPVPVLDLETLVAVAEAPRHEPNILY
                     VGRLAPEKRLDLWLEAARRIRDEVSAATFDIVGSGGERGTLERQAAELGLASAVTFHG
                     HARREELPEHFARARVLMLSSDHEGFGRVLLEAMAAGVAVVSTATAGAREIIAEGSGA
                     GALVPVGDAAALAAAASPLLTDDGHHCRMVDAARAHIRGRYDPMQLAVGWVEMLIDVA
                     ERGP"
     gene            8900..>10008
                     /locus_tag="PK264_11270"
     CDS             8900..>10008
                     /locus_tag="PK264_11270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD76502.1"
                     /translation="MTNVFPPLIGGPATFADRLAHVLTGRGHKVSVVCSSQSAKDPGD
                     AARPFRVVRVSLANRYAYEAKVRAVLVREMLAHRRILVAGLESYVLDAARVAPRRYIL
                     RVPGDTVWESARNYGETTLGFDEFQDAASPPAVVAGIIARRRQYLSRAVTVVTPSHYL
                     EGVVRRWPGCPADLRTIINGVSLSDYPDREPSPRNGRALKALFVGRLTNWKGVETLLL
                     ASVGLSGVEITIVGDGPELPLLKALNAQLGGAGGGRSVRFLGRQSPDAVKASMLEHDV
                     LVLPSNYEGMSHTLLEGCAAGLVPIVSAIGGNLEVITDGQHGLAVPYGDPDALRRALA
                     ALAADDVLRQRLALAARHRAGDFPFAATVDGYANL"
     assembly_gap    10009..10020
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10021..>10382
                     /locus_tag="PK264_11275"
     CDS             <10021..>10382
                     /locus_tag="PK264_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76503.1"
                     /translation="TDLSSSPQRLAVTHVSPFGDYEGRIAFSGAENHLFQLMAGQRAA
                     GLDVELLMLVVHDGPRLEAKARDLTAAGIPVTRLVYDRSMAGAIGRAAWVAQLPRLIR
                     IMGARSDRIIHTHQPHAS"
     assembly_gap    10383..10432
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10433..11210
                     /locus_tag="PK264_11280"
     CDS             <10433..11210
                     /locus_tag="PK264_11280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD76504.1"
                     /translation="IHNDEPHFANATWRARLKALERITGATIAISDRVKSHLVDNVGL
                     DTDRITVIPYGIEPPSTIDRAGARAALGLDPAAFVIGFVGRFVPQKDLGILLDAFGRV
                     EGAELCLVGAGDEEAALRAHALRLGQKRVHFAGARANGADLMAAFDVFALSSKWEGLG
                     LVLLEAMSRGVPIAATRGGAIPEVLDSGRLGLLSDVGDAGALAANMQRLRDDPGLARE
                     FIERGHAAIRERYSVAAMVNATTAVYERAFGSAATRGGPS"
     gene            11207..12025
                     /locus_tag="PK264_11285"
     CDS             11207..12025
                     /locus_tag="PK264_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRD76505.1"
                     /translation="MSVTRLLSAARETLAIAVRLGQATGRPDRSTVYAARRLVDRRRP
                     VDLTWRGMQLAARGMDWAALQEVLIENEYGALTPLLKSTPEPVVLDLGANIGTFSLFV
                     LAHAPRARVFSYEPSAATFAVLAATAAKNPTYRWTAVQAAAWRADESVTFANAAASTA
                     GRISGEGDERVAGRSFASILETCGGTADIAKLDIEGAEEAVLADQPAALLDRIGTIVV
                     ELHPGRCDTDRVARTLMNSYGTVYAIPGRRSSKPLLLATRSAIALGLPVYGAAA"
     gene            12066..12833
                     /locus_tag="PK264_11290"
     CDS             12066..12833
                     /locus_tag="PK264_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76506.1"
                     /translation="MLARLNAAVRSRMNRILGRDFRSELLRRFSGRMGARKVIEHDGV
                     RRLDIDYADPAARATYREIQTFGNRQKLGVVFASEPNIAAMARLAAARVGPVGLVICH
                     GTRNGAEQRWFKANLPGTPRVIGTEISDTATEFPDTIQWDFHEQNPEWIGRADIVYSN
                     SWDHSIDPERMFRNWMECLSENGIMLIEHTSYHDGREPDLLDPFGATRDGLVRLLDRV
                     GKSEFRVVEIFTDLPDTSAGRCAVVVARADREQRTRQ"
     gene            12830..>13471
                     /locus_tag="PK264_11295"
     CDS             12830..>13471
                     /locus_tag="PK264_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD76507.1"
                     /translation="MIVSPRPPSRNRLSAFSDKRLSYLRALEHELAPTIPLVGRVLDF
                     GGGQDSSYNSRLTSGVTIDSLNVDTARSPTYLHDANTPFPIADATYDTIVSFNTLEHV
                     RRDEFALGEIVRILKPGGTLHIIVPFLYRVHASPSDYHRHTAFWWEAALADLGIGPEN
                     SRIEPMMWGRLTSSFSFLEYTRLRFLRRLPLWADIWLKGMGDDEYPLGYYVSGK"
     assembly_gap    13472..13510
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13511..>14079)
                     /locus_tag="PK264_11300"
     CDS             complement(<13511..>14079)
                     /locus_tag="PK264_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76508.1"
                     /translation="VSDTRDLSTDRSGDTLVVAAFGGDTTTGLYRVARQAYDNLAGLT
                     DAVHQFYTPTIVDALARGRTDAYRRHRRRLMAIGTVAAVVSVLASWLLLRPIAAAGWP
                     QYLPALPAFDIFAALLVATLGVHGWLWPAVVSAGRVGQFGILALSGAAAQVAAIAVLG
                     ATGGLSPASAAATAWLAALINYGPLLAAG"
BASE COUNT         2220 a         4840 c         4503 g         2415 t
ORIGIN      
        1 agcttcgagc tcggcatcct cgtcagtgtg atcgcggtcg tcggcatcct gccctacatc
       61 tcgcttcagc tgaaggcgat ctcggcgagc ttctcgatcc tcgtgcagta tcccgaggtc
      121 aggatgccgg ccagcacggc cgcctcgatc ctcagcgata ccgagctcta cgtcacgctg
      181 gcgatggcga cgttcgcgat cctgttcggc acgcgccata tcgatgcgag cgagcgtcac
      241 gagggcatgg tcgcggccat cgccttcgaa tctggcgtga agctcatcgc cttcctcgcc
      301 gtagggctgt tcgtcaccta cggcgtcttc aacggcttcg gcgacatttt cggccgggcg
      361 gcgtccgacg cgcgactgca gcgcctgttc acgatcgatg gcatggccgg ccacgtgagc
      421 tggctcaccg tgctgctcct ggcgatggcg gcgatcgtgg tgctgccgcg ccagttccag
      481 gtgctcgtcg tcgagaatgt caacgagggc cacctgcggc aggcgatgtg gctgttcccc
      541 ctttatctgc tgctgatcaa catcttcgtg ctgccgatcg ccttcgccgg cctgctgcgg
      601 ctcccggcga cgggcgtcga tgccgatacg ttcgtgctca ccatcgccat gcaggagcgc
      661 gcgccgatgc tggcgctgct cgcattcatc ggcggcttgt cggccgcgac cggcatgatc
      721 atcgtcgaga ccgtcgcatt ggcgaccatg gtttcgaacg atctcgtgat gccgctgctg
      781 ctcaggatcg gccggctcca cctctccgag cggcgcgatt tgacgggcat cctgctcgcg
      841 atccgccgcg gcacgatcct gctggtcctg ctgctcggct acgtcttcgc aagcaccgtc
      901 ggcggctcct atgctctggt gacgatcggg ctcgtgtcgt ttgtggcggc gcttcagttc
      961 gcgccaccga tccttgcggg tctctactgg aagggcgcga cgaaggcggg cgcgctcgcg
     1021 gcgctctcgg gcgggtttgc ggtctggctc tatacgctgc tgctgccgtc gttcgcgaag
     1081 tcgggttggc tgccgtccgc gttcgtcgac gccgggctct tcggctcggt cctgttgaaa
     1141 ccctacgcgc tcctcggtct cgaggggctc gaccccatct cgcacgcgac gatttggacg
     1201 atgctggtca atgtcggcct gctgattggc gtctcgctgt tcacttccca gtcaatgatc
     1261 gagcgcagcc aggccgtgca gttcgccgac gtgttcaagt cggcctcgag cgccacgcgc
     1321 gtctggcgcg ggcgcgtgac gaaggcggag ctctttgcgc tgctcggccg cttcgtcggc
     1381 gacgccaggt ccgatacggc gctcgcacgc tatgagcagg agatcggacg cacgctcgat
     1441 cgcaccgcgg ccgcagatgc cgcgctcgtc gcctacgtcg agcgtcagct cgccggcgcc
     1501 atcggcgcgg cctcggcgcg catcatgatg gcctcggtgc tgcgcgagga gatgcaggac
     1561 atcgatgcgg tgacgcgcat gctcgacgag gcgtcgcagc tcgtcctcta ctcgcgccgg
     1621 ctcgaggaga agtcgcagga gctcgaggcg gcgtccgctg agctgcgctc ggccaacgag
     1681 cgcctcaagg agctcgacaa gctcaaggac gacttcatgg cgaccgtcag ccacgagatc
     1741 cgcacgccgc tgacgtcgat ccgctcgttc agcgagatcc tgcgtgacaa ccccgacctc
     1801 gacaacgagc agcgccagga gttcgtcacc atcatcgtgc aggaaagcga gcggctgagc
     1861 cgcctcatca acgacatcct cgacctcgcc aagatggagg cggggacgac cgactggcag
     1921 atcgagctgc aggacgcgaa gccgatcttc gagcaggcgc tcgcagcgac cagcggtctc
     1981 ttcgccaagg cgcccggaat taggctcggc atcgagatcg cgccggacct gccgaaagtg
     2041 agcgtcgacc gagatcgcct catccaggtc gtcgtcaacc tcatctcgaa cgcagtcaaa
     2101 ttctctgaca agggcaaggg cgaaatccgt ttcaaggcct ggagacacga caacacgctg
     2161 cgtgtcgacg tcgcagacaa cggcatcggc attgcgccgg cagatcaggc gcgcgttttc
     2221 gagcgcttcc agcaggccgg gaatatcctc accgacaagc ccgaaggcac gggccttggc
     2281 ctgcccatca cgcgccagat cctgcaacgc ttcggcggag acatttggct ccagagcgct
     2341 gtcggcaaag gctccgtctt ctcgttcagc atccccctcg cggccgccgg cgacgcgcgc
     2401 ggctgagtgc ccaagtcctc ccgatatcga ccatcgttct aagcggagtg gctaactggt
     2461 gctgcatcgc gcgcatacct atggtgcccg ccagtgttgg ctgtcggatc gggatcgagc
     2521 cgcactcaga aagcgaccaa gtccgcgaac tcgtaccatt ggaggagatt gatcgatggc
     2581 gaccgaagcc acactgcata accccgaagc acgcagtcga ccgatgactg gcgaggagcg
     2641 aaaggttatc ctcgcctcct cgctcggcac cgtcttcgaa tggtatgatt tctacctcta
     2701 cggcgccttg gccgtgatca tcggcgcgca gttcttctcc gcctttgatg tgacgacgcg
     2761 caacgtcttt gcgctgctcg ccttcgccgc cggcttcctc gtccgtcctt tcggcgcgct
     2821 cttcttcggc cgcctcggtg acctcatcgg tcgcaaggcc acgttcctca tcaccatcct
     2881 gctcatgggc acgacgacct tcatggtcgg cctgctgccg agctacgacc agatcggctg
     2941 ggtcgcgccg gtcgtgctga tcgctctgcg catgggacag ggcctcgcgc tcggcggcga
     3001 gtacggcggc gccgcggttt atgtggccga gcatgcccca cagggccggc gcggcttcta
     3061 cacctcgtgg atccagacga cggcaaccct gggcctgctg ctctcgctcg tcgtcatcct
     3121 ctcgatccgg ctctatatgg gcgaggaggg tttcagcaag ggcacgggct atccgattgc
     3181 cggctggcgc attccgttcc tggcgtcgat catcctgctc gcgatctcag tgtggatccg
     3241 catgcagatg cacgagagcc cggccttcaa gaagatgaag gaggaaggct cgctttccaa
     3301 ggcaccgctc tcggaagcct tcggacagtg gaagaacctg aaggtcgtga ttttcgccct
     3361 cctcggtctc gtcgctggcc aggcggtcgt ctggtacacg ggccagttct atgccctgtt
     3421 cttcctgcag agcatcctca aagtcgacct gttcacggcc aacgtgctga tcgcctggtc
     3481 gctgatcttg ggaaccggtg gcttcatcgt gttcggcact ctgtcggaca agatcggccg
     3541 caagccggtg atcctcgcag gctgcctgat cgcagcactc acctacttcc cgctcttcaa
     3601 gatgatgacg gcgacggcca acccgaagct cgccgaggcc atcgagaagg tgaaggtgca
     3661 ggtgaccgct gatccggcga agtgcggctc ggtgttcgac cccgtcggca tccgctcctt
     3721 ctctgagccc tgcgacgtcg cccgagtgtc gctggcccgc gccgccatca cctataatct
     3781 ggtgcccggc cccgccggat cgccagccaa ggttacgttc aacggcaagg acgtgccgat
     3841 cgaggccgcc atcccgaaga acatctcggg cttcacagcg gcggccatcg ctgccggcta
     3901 cccgaaggcc ggcgacacca gcatcgtcaa gaccacgtcg gtcatcccga gcggcgcaca
     3961 ggcctggacg atcatcggca tcctgacgct gctcgttgtc tacgtgacga tggtgtacgg
     4021 gccgatcgcg gcggcactcg tcgagttctt cccgacccgc atccgctaca cggccatgtc
     4081 cctgccctat catatcggca acggctggtt cggcgggttc ctgccgccga cctccttcgc
     4141 tatggtggcc gcgaccggcg acatctacta cggcctctgg tatccggtcg tcgtcgcact
     4201 cgccacattc gtgatcggcc tcttcttcgt gccagagacc tacaagcgag acatcttcaa
     4261 gggttgatcc ctcgccgcag cagtgatcga ctgttacaga ccttctacct gccggggcgc
     4321 gcaacgcccc ggcagaattg ttctcccggg ttatctgcct atctcagtag gcacgacatt
     4381 ttcggctcgg aacctgcaca tgttgatggc gctactggca gacgtgcatg ccaaccgtga
     4441 ggccttcgag gcttgcctcg tggacgcccg gcgctcgggc gccgaacgct tcatcctgct
     4501 cggcgatctc gtcggctacg ggcccgatcc ggtttgggcc gtcgagacgg cggcacgcct
     4561 tctcgatgac ggcgcgctcg ccgtgcaagg caatcatgat gccgccgcgt ccggcgctgc
     4621 gacctcgctc aaccccgtcg cccgggcagc gatcaggtgg acggccgagc agctcggccc
     4681 cgcgcataag cagttcctcg ccgacctgcc gctcgatctc aacgaggacg accgcctcta
     4741 cgtgcacgcc agcgcctcgt cacctgagga ctggatctac gtgctctcgc cgcgcgaggc
     4801 cttcgcgagc ttccgcgcga cggcccagcg catcaccttc tgcggccata cgcacctccc
     4861 cgcgctcttc aacgagagcg cgagcagcct cccccagcag cacacgcccg cggacggccg
     4921 gcccgtgccc ctcctcgtgc agcgccgctg gctcgccgtt ctcggcgccg tcggccagcc
     4981 gcgggatcac aatcccgcag cgtgctacgc gctcttcgac gacgagacgc gccgcctcac
     5041 ctacatcagg gtgccctacg atgccgatgt gacggcgcgc aaggtgcgcg cggccggatt
     5101 gccggatgct ctcgccgtcc gattggtctc gggaagctga cgtatggcga aggcatcgct
     5161 cagcatcggc atggtcctcg acggcttcag gctcgaggag cgcctgcacc gcggcgggat
     5221 ggcgacgatc tggcgcgtga cgcgcccgga catcgacgat cccatcgtca tgaaggtccc
     5281 attcctcgac cacgagggcg acctttctct gctcgtcggg ttcgaggtcg agcagatgat
     5341 catggccgag ctggccgggc cgcacgtccc gcgcttcatc gccaatggcg acttcaccgt
     5401 gcagccttac attgtcatgg agcgcctgcc gggcaacaac ctgcacaaga tgctgttcga
     5461 tacgccgcgt ctcgagatcg aggacgcgat cagcaagggc accgccattg ccgaggccct
     5521 gggggacctt cacaaccagc acgtcctgca tctcgacctg aagccggcga acgtgctgtt
     5581 ccgaccgtcg ggagaggccg tactcattga ttttggcctg tcgcggcacc tgctgctgcc
     5641 cgatcttctc gaggagcagt tccatcgccc gaccgggacg acggagtaca tggcgcccga
     5701 gcaactcgcg cgcgtgcgct cggacaagcg gagcgacctc ttcgcgctcg gtgcgatcat
     5761 ctaccagatg gcgaccggtg agctgccctt cggtgcgccg ggccgcatca agcaggtgcg
     5821 tcagcgcgtg tggcgcgatc cggtgccgcc gcgggcgctc cgacccgagc tgccggcgcc
     5881 gctgcaggag gtcatcatca agtgcctcga gccgcttccc gacaagcgct accgcgacgc
     5941 cgaggaactc gtcttcgatc tacgccatct cgatctcgtc gaactcaccg agcgcgccgg
     6001 gcggcaggcg cgcgctgatg cccgcaccgc cttcgggcgc tggatgcgcg ccaagtcgac
     6061 gctgcgcgag atccagagca cggcgacgaa gccgccgccg cgcacgccga tcatcctcgt
     6121 cgccgtcgac ctccgaccca ggttcgtcga gcttcgcaat tcgctgctcg aggccgccgt
     6181 ctcggttctc gccaacatgc ctggcgcccg gctcgcttgt ctcaacgtga tggagacctc
     6241 gctcatctcg atcgacgaga atgtcgatgc cgcgggcgac aacatccacg tccagcgcat
     6301 ggcggagctc cgacagtggg cagcgccgct gcagttgcca cgaggcaaga tcacttatca
     6361 cctggtcgag gctcgcaaca ttgcaggcgc gatcctggat ttcgcacgct ccaacaaggt
     6421 ggatcacctg gtgatcggtg cgccgaccgc aggcggggcg gcagccagcc ggctcacggc
     6481 ccagatcacg gccgaatcgc cctgctccgt ctctgtcgtc cgggcgagcg cggaccgggc
     6541 ggacctggtg ccggtccgtt gaggcccggc atacgggccc gcaatttccg caagagcaac
     6601 ttccgcaaaa gttgccaagc cagcgtcttt atactaatcg ctgcgtctcg ccgggaccat
     6661 caggcgcccc aacgtccaca agaattcgcc aggaatttcc atgggatggg agctttcagg
     6721 acggcgcttg atcgtcacgg gcggcgccgg gttcatcggc tcgcacctct ctgagcggct
     6781 gctcgcgaag ggcgccgagg tcttgtcggt cgacaactac ttcaccggcc ggcgcgccaa
     6841 catcgcgcat ctcctgcaaa atccctcctt cgaggcgatg cgccacgaca tcacttttcc
     6901 actctacgtc gaggcggacg gcatcctgaa cctcgcctgt ccggcctcgc ccgtgcacta
     6961 ccagcacgat cccgtgcaga cgacgaaggt gagtgtgcac ggcgcgatca acatgctggg
     7021 actggcgaag cgcctgcgct gccgcatcct ccaggcctcg acgagcgagg tctacggcga
     7081 tccgaccgtc cacccgcagc ccgaggagta ctggggcaac gtgaacccga tcggcccgcg
     7141 ctcctgctac gacgaaggca agcgctgcgc cgagacactc ttcttcgact accgccgcca
     7201 gcacaacctg ccgatcaaga ttgcgcgcat cttcaacacc tacggcccgc gcatgcagcc
     7261 gaacgacggg cgcgtcgtct cgaatttcat cgtgcaggcg ctgcagggcg agccgatcac
     7321 gatctacggc gacggggctc agacgcgctc gttctgctac gtcgacgacc tcgtcgatgg
     7381 cctcgtccgc ttgctgcaga cgggcgagga catgaccggc ccgatcaatc tcggcaatcc
     7441 gagcgagttc acgatccggg cgctcgccga gaaggtgatc gagatgaccg gcgcgcgctc
     7501 caagctcgag ttccgcccgc tgccgcacga cgatccccgc cagcgccagc cggacatcac
     7561 caaggcaaaa tccatcctcg gctggcgtcc gaccgtcgag ctcgaggacg gcttgaaaga
     7621 gacgatcggc tacttccggc ggcttctggc gcagtgagcg gcgctccgtc gcaacctgag
     7681 gcacgcacgg cagcacgccc gctgcacatc gtccaggtga gtttcgatgc ctcgctgctc
     7741 gacggcggcg aagcgagcga ttcgatcgcg cggcagaagc tctattcgcg cctgcttgcc
     7801 gagcgtcggc cgggctcacg catgaccatc gtcgtgctga acgcgccagc gacggcagtg
     7861 gcctgctcgt gggacaatct ccacgtcgtg ccgatctccg ggcgctgggc gagcctccgg
     7921 cgcctcccgg cagcccttcg cgcgctcgac cgcgaggcgg cgatcggcgt gatagcggcg
     7981 cagtcgccct tcgaggatgc ctgggcggcg ctcatgttcg cgcgtcgcag gattccggtg
     8041 gtcgtccagg tgcacttcga cgtcctcgcc gccgaggcgc tgccacgggg cagcatgatt
     8101 cgccgactgg cggggaaggc gcggcagcgg ctggccttgc gatcacttcg acggttcgcg
     8161 tccgtccgcg tcgtcgccgc cgcgatgaag cggcgcctcg aggcggccgg cgctcgcgat
     8221 gttcgcgcga tccccgtgcc cgtgctcgat ctcgaaacgt tggtcgccgt cgctgaagcg
     8281 ccgaggcacg agccgaatat cctctacgtc ggccgtctcg cccctgagaa gcgtctcgat
     8341 ctctggctcg aggctgcgcg gcggatcagg gacgaagtat cggcagcgac gttcgatatc
     8401 gtcggctcgg gaggagagcg gggaacactg gagcgtcaag ccgccgagct cggcctcgcc
     8461 tcggccgtga cgtttcacgg ccacgcacga cgcgaggagc taccggagca cttcgcacgc
     8521 gcccgcgtcc tcatgctgtc gtccgatcac gagggcttcg gtcgcgtcct gctggaggcc
     8581 atggcggcag gtgttgccgt ggtatcgacc gcgacggccg gggcgcgcga gatcatcgcg
     8641 gagggaagtg gcgccggagc gctcgtacca gtcggcgatg cggcagcact ggccgccgcg
     8701 gcgtcgccgc tcctcacgga cgatggccac cattgccgca tggtcgacgc cgcacgcgcg
     8761 cacattcggg ggcgctatga ccccatgcaa ctcgctgtgg gctgggtcga gatgttgatc
     8821 gacgttgccg agcggggacc ctaaagagag cctggacgat tgcttgtgaa aggcgcccgc
     8881 attggctcga gtgctcgtcg tgacgaacgt gtttcccccg ctgatcggcg ggccggcgac
     8941 attcgccgac cggctcgcgc acgttcttac aggccgcggc cacaaggtaa gcgtcgtctg
     9001 ctcgtcgcag tcggccaagg acccgggcga tgccgcgcgg ccgttccgcg tcgtccgcgt
     9061 ctcgctcgcg aaccggtatg cctatgaggc caaggtgcga gcggtgctcg tacgcgagat
     9121 gctcgcccat cggcgcatcc tcgtcgcggg cctcgaaagc tacgtgctcg acgccgcgcg
     9181 ggtcgcgcca cgccgctaca tcctgcgcgt tcccggcgac acggtgtggg agagcgcccg
     9241 caactacggc gagacgacgc tcggcttcga cgagttccag gacgcggcct cgccccccgc
     9301 ggtcgtcgcc ggcatcatcg cccgacgccg gcagtatctc tcgcgtgccg tcaccgtcgt
     9361 cacgccatcg cactatctcg agggcgtggt gcgccgctgg ccgggttgtc cggcggacct
     9421 gcgcaccatc atcaacgggg tatcgctgtc ggactatccg gatcgcgagc cgtcgccgcg
     9481 caacggccgc gcgctgaagg ccctgttcgt cggccgcctc accaactgga agggcgtcga
     9541 gacgctgctg ctcgcatccg ttggcctgag cggagtcgag atcacgatcg tcggcgatgg
     9601 gccggagctg ccgttgctca aggctttgaa cgctcaactc ggcggagcag gcggcgggcg
     9661 cagcgtgcgc tttctcggcc ggcagtcccc cgacgccgtg aaagccagca tgctcgagca
     9721 tgacgtgctc gtgctgccgt cgaactatga gggcatgtcg cacacgctgc tcgaagggtg
     9781 cgccgcaggg ctcgtgccca tcgtctctgc catcggcggc aatctcgagg tgatcacgga
     9841 cggccagcac gggctggcgg taccctatgg cgatccggat gcgctgcggc gagcccttgc
     9901 cgcactcgcc gccgacgatg tgctgcggca acggctcgcg ctcgccgccc ggcaccgtgc
     9961 gggcgacttt cccttcgcgg caacggtcga cggctacgcc aatctcatnn nnnnnnnnnn
    10021 accgatctct ccagctcacc gcagcgactc gcggtcaccc atgtatcgcc gttcggcgac
    10081 tacgaggggc gcatcgcctt ctcgggcgcc gagaaccatc tcttccagct gatggcgggc
    10141 cagcgggctg cgggcctcga tgtcgagctg ctcatgctgg tggtccacga cgggccgcgg
    10201 ctcgaggcga aggcgcgcga tctgacggcg gccggcattc cggtgacgcg gctcgtctat
    10261 gatcgcagca tggcgggcgc gatcggccgg gcggcctggg tcgcacagct tccgcgcctg
    10321 atccgcatca tgggcgcgcg cagcgatcgc atcatccaca cgcatcagcc gcacgcctcc
    10381 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncatccaca
    10441 acgacgagcc gcattttgcg aatgccactt ggcgggcgcg gctcaaggcg ctcgagagga
    10501 tcacgggtgc gacgatcgcc atttccgacc gggtgaagtc ccacctcgtc gacaacgtcg
    10561 ggctcgacac ggaccggatc accgtcatcc cctacggcat cgagccgccg tcaacgatcg
    10621 accgggcggg cgcacgcgcg gcgcttggcc tcgatccggc ggccttcgtc atcggcttcg
    10681 tcggccggtt cgtgccgcag aaggatctcg gcattcttct cgacgcattc ggcagggtcg
    10741 agggcgcgga gctctgcctc gtcggcgcgg gcgacgagga ggcagcactg cgcgcgcatg
    10801 ctttgcggct cgggcaaaag cgcgtgcact tcgccggagc gcgggcgaat ggcgccgacc
    10861 tgatggcggc cttcgacgtg ttcgcgctct cctcgaagtg ggaagggctc ggcctcgtgc
    10921 tgcttgaggc gatgtcgcgg ggcgtgccga ttgcggcgac ccgcggcggc gccattcccg
    10981 aagtgctcga tagcggccgg ctcggtctgc tttccgacgt tggggacgcg ggcgcactcg
    11041 ccgcgaacat gcagcggctc cgcgatgatc caggactcgc gcgcgagttc atagagcggg
    11101 gccacgcggc catccgcgag cgctacagcg ttgccgccat ggtcaacgca acgacggcag
    11161 tgtacgagcg agcgttcgga tcggccgcaa cacgaggggg cccgtcatga gcgtgacacg
    11221 cctgctcagc gccgcgcgcg agacgctggc gattgcggtc aggctcggcc aggcgaccgg
    11281 caggccggac cgctccacgg tctacgctgc gcgccgcctc gtcgatcgac gccggccggt
    11341 cgatctcacc tggcgcggca tgcagctcgc agcgcgcggg atggactggg cggcgctgca
    11401 ggaggtgcta atcgagaacg agtacggcgc actcacgccg cttctcaaga gcacgccaga
    11461 gcccgtcgtc ctcgatctcg gcgccaacat cggtacattc tcgctctttg tgctggcgca
    11521 tgcgccgcgg gcgcgcgtct tctcctacga gccgtcggct gcaaccttcg ccgttctggc
    11581 ggcaaccgcg gcgaagaacc ccacctatcg ctggacggcc gtccaggcgg cggcctggcg
    11641 ggccgacgag agcgtgacgt tcgccaacgc cgccgcgagc accgcaggac gcatttcggg
    11701 cgagggagac gagcgcgtcg cgggccgctc gttcgcctcg atcctcgaaa cctgcggcgg
    11761 caccgcggac atcgccaagc tcgacatcga gggcgccgag gaggccgtgc tcgccgacca
    11821 gcctgcggcg ctgctcgatc gcatcggcac gatcgtcgtc gagttgcacc ccggccgatg
    11881 cgacacagat cgcgtcgcca ggacgcttat gaatagctat ggaaccgtct atgccattcc
    11941 ggggcggcgg tcctcgaagc cgctgctgct cgcgacccgc tcggcaatcg ccctcggctt
    12001 gccggtgtat ggcgcggcag catgagatgg gcgtggcacg tcgtcgtgtt gatcgggagg
    12061 agcatgtgct cgcaagactg aatgcagccg tgcgcagccg gatgaaccgg atcctcggcc
    12121 gtgatttccg ttccgagctg ctgcgccggt tctccggccg catgggcgca cgcaaggtca
    12181 ttgagcacga tggggtccgc cggctcgaca tcgactatgc ggacccggca gcgcgcgcga
    12241 cctatcgcga gatccagacg ttcggcaaca ggcagaagct cggcgtcgtc ttcgcctcgg
    12301 agccaaacat cgcggcgatg gcgcgacttg cggcggcccg cgtcggtcct gtcggcctcg
    12361 tcatctgcca cggcacacgc aacggcgctg agcagcgctg gttcaaggcc aatctgccgg
    12421 gcacgccgcg cgtcatcggc accgagatct cggatacggc gactgagttt cccgacacga
    12481 tccagtggga cttccatgag cagaacccgg agtggatcgg acgcgccgac atcgtctact
    12541 caaacagctg ggatcacagc atcgatcccg agcgcatgtt ccgcaactgg atggagtgcc
    12601 tgtcggagaa tggcatcatg ctgatcgagc acacgagcta tcatgacggg cgcgagcccg
    12661 atctgctcga cccgtttggc gcaacgcgcg acggcctcgt ccggctcctc gaccgcgtcg
    12721 gcaagtccga gtttcgcgtg gtcgaaatct tcactgatct gcccgatacg agcgccggcc
    12781 gctgtgcggt cgtggttgca cgagccgacc gggagcagcg caccagacaa tgatcgtttc
    12841 tccgaggcca ccgtcccgca accggctctc cgcattctcc gacaagcggc tttcctacct
    12901 gcgcgcactt gagcacgaac tcgccccgac cataccgctc gtcggccgcg tactcgattt
    12961 cggcggcgga caggattcga gctataattc acgcctcacc agcggcgtga cgatcgacag
    13021 cctgaacgtg gatacggcac gctctccgac ctacctccac gacgccaaca ctcctttccc
    13081 gatcgcagac gcgacctacg acacgatcgt ctcgttcaac acgctcgagc acgtccgccg
    13141 cgacgagttc gcgctcggcg aaatcgtgcg catcctgaag cctggcggca cgctgcacat
    13201 catcgtgccg ttcctctacc gggtgcatgc ctcgccctcg gactatcatc gccacacggc
    13261 attctggtgg gaggcagcgc tcgctgatct cggcatcggc ccagagaact cgcgcatcga
    13321 gccgatgatg tgggggcggc tcacatcctc gttctcgttc ctcgagtata cgcggctgcg
    13381 cttcctgcga cgcctgccgc tctgggccga catctggctg aaaggcatgg gcgacgacga
    13441 gtatccgctc ggctactacg tctcgggcaa gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn gccggctgcg agcaacggcc cgtagttgat cagagcggcg agccaggcgg
    13561 tcgccgccgc cgatgctggt gagagcccgc cggtggcacc gagcacggcg atcgccgcca
    13621 cctgcgccgc ggcacccgag agagccagaa tgccgaattg gcccacccgc cccgccgaga
    13681 cgaccgctgg ccatagccag ccgtgcacgc cgagagtcgc gacgagcagc gccgcgaaga
    13741 tatcgaaggc cggcagcgcc ggcaaatact ggggccagcc ggctgcagcg atcggccgga
    13801 gcagtaacca ggacgccaga acggagacca ccgccgcgac ggtgccgatc gccatcaggc
    13861 gccgccggtg ccggcggtag gcgtcggtcc ggcctcgcgc gagagcgtcg actatggtcg
    13921 gcgtgtagaa ctggtggacg gcgtcggtga gcccggcgag gttgtcgtag gcctgccgcg
    13981 cgacgcgata gaggcccgtg gtcgtgtcgc caccgaacgc cgcgacgacg agcgtgtcgc
    14041 ccgatctatc ggtggagagg tcgcgggtgt ccgacacgg
//
LOCUS       DAORNH010000095        14063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3271_length_14063_cov_2.302970, whole genome shotgun sequence.
ACCESSION   DAORNH010000095 DAORNH010000000
VERSION     DAORNH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_14063_cov_2.302970"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    275..306
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(376..1140)
                     /locus_tag="PK264_11305"
     CDS             complement(376..1140)
                     /locus_tag="PK264_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HRD76509.1"
                     /translation="MRYERVPLHTDAGLGARARIGLLVLRTDQTFESDLRQVIQRLPD
                     VAVYAARLWNDTEITRETLLAMKPLIADTARLLPAEWSFKSIGFACTSGAIVIGEAEV
                     ARLVRSVHSAAAVTDPVTAVIAALRALGVRRPALITPYARPVNDTIISGLAARGIEVP
                     ALASFEEPDDGVVARITQQAIADTARRVGAASDVDSVFVSCTSLRTLDVIGAIEQAIG
                     MPATSSNHALIWHMLRLAGIQEPLDGLGRLYRVAPG"
     gene            1374..2174
                     /locus_tag="PK264_11310"
     CDS             1374..2174
                     /locus_tag="PK264_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRD76510.1"
                     /translation="MLGELWLYAGVMLAGLVSFLSPCVLPLVPPYLGYIGGTTIEQMT
                     ADHGLDRSASRKVIIAALVFVLGFTTVFVSLGAAASMAGQFLATWKRELGWVAGGVII
                     LFGLHFLGIFKIPLLYREARYHGRVEGASLAGAYLMGLAFAFGWTPCIGPVLSAVLGL
                     AMSENSLGAGVKLLFAYSLGLGIPFVLAAVAIRPFLGFMGRFRRHLGRVEKIMGALLV
                     LTGVLFIFGGIGWMGQWLIDNVPILARIEEMFTSDKLRIEIMKKGAEK"
     gene            2196..3032
                     /gene="thiD"
                     /locus_tag="PK264_11315"
     CDS             2196..3032
                     /gene="thiD"
                     /locus_tag="PK264_11315"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRD76511.1"
                     /translation="MTTARIPTALTIAGSDSSGGAGIQADLKTFTALGIYGASVITAL
                     TAQNTLGVQAVAAVEPGFVRRQIASVMDDLDVRAIKTGMLANAGVVAAVVDGLAARQT
                     NRNAIPLVVDPVMVATSGDSLLQADAIGIITRRLFPLASVVTPNLPEAARLLGTSEAR
                     TTDEAIDQARAILQLGPGAVLVKGGHGRGSEAVDVLVNHSGIELLTAERIATHNTHGT
                     GCTLSAALAAGLADGLGLIDACRCAKAFVSDALRSGAVRPVGHGHGPVDHIFAIRNRR
                     RQ"
     gene            3597..4280
                     /locus_tag="PK264_11320"
     CDS             3597..4280
                     /locus_tag="PK264_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HRD76512.1"
                     /translation="MALTFRCSARSGAETRSFEPTALVVAGWTGRDEKSLQHHIEELA
                     AIGVPRPSSVPVFYRNAIANVLQADRIEVLGPDTSGEVEPVILAMPDGLWLTVGSDHT
                     DRKAETMGIALSKQLCPKVLGREAWRLDEIETHFDEIRIRAFAVIGGKRVAYQDGTLA
                     TLRAPSDLIQRYGKGKPLAPGTVMMGGTFGAIGGIRPADRFEMELDDPRTGRVIRAAY
                     AIETLPVVT"
     gene            4305..>4632
                     /locus_tag="PK264_11325"
     CDS             4305..>4632
                     /locus_tag="PK264_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRD76513.1"
                     /translation="MTAAMPTIADVRAARARLAPHIMPTPLIEHPALNALAKGRVLLK
                     CETMMRVGAFKFRGAYNAASQVDRVRFPGGIVACSSGNHAQGAAAAATLLGMHSAIVM
                     PADAPAL"
     assembly_gap    4633..4779
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4780..5294
                     /locus_tag="PK264_11330"
     CDS             <4780..5294
                     /locus_tag="PK264_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRD76514.1"
                     /translation="GLEIMEEAKARGLVPDAVLAPCSGGGLIGGIAIAVKDASPKTEV
                     MSVEPAGFDDLARSLASGKPEKNAKLSGSICDALLAPSPGSVTFPVAKALLTRGLVVT
                     DDEARVAIRFAFHELKLVLEPGGAVALAAVLARKLPTEGRTVAVVLSGGNIDPEMFAE
                     IIRNDDKLDS"
     assembly_gap    5343..5426
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5594..6412
                     /locus_tag="PK264_11335"
     CDS             5594..6412
                     /locus_tag="PK264_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76515.1"
                     /translation="MKRVLVTGAAGGVCTWLRKLLPEVYPDIVWSDRVKPKDLASNET
                     FIQAELSKPAEIEAACKGIDGIVHLGGYSVEGPWETILEANIVGCYNLFEAARKAGVK
                     RVIFASSNHAMGFHPRARPIDTDVTGRPDSRYGVSKLFGEGLGAMYAYKHGIGVTCLR
                     IGNVADQPIDVRRLSIWLKPSDLVELIRIGLERPGIVFEVMYGVSDNEASWYDNERAF
                     RLGYRPKGRAEDFRAAAEAAQKKIAADPVADFYQGGSFCSAEYSADFEDLKNKS"
     assembly_gap    6473..6495
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6496..7200
                     /locus_tag="PK264_11340"
     CDS             <6496..7200
                     /locus_tag="PK264_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76516.1"
                     /translation="GGAIAGRLAEEGAIVGIFDKNGYSAEAAAEKLGQGSIAVACDIT
                     DHAWVVKAVAEFEAKAGPTHALVNNAGWDKFAFFLDTKPELWDQLIAINLKGPINMHH
                     AVLKGMAQRRAGRVVNIASDAGRVGSSMEAVYSACKGGIISFTKTMAREMARSGVTLN
                     AVCPGPTDTPLLADFMGEGEKGKKIHDALVSAIPMKRLGRPEDLAGIVTFLASDEAAY
                     ITGQVISASGGLTMVG"
     gene            7234..8430
                     /locus_tag="PK264_11345"
     CDS             7234..8430
                     /locus_tag="PK264_11345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD76517.1"
                     /translation="MYPILKGYRIVDVTTIVLGPYGTQILGDLGAEVIKVEAIEGDLN
                     RWTLPAAEPGVGAVFANNNRNKRSLAVDLKQDAGKEVLRRLVGTSHAFVHNMRASAIE
                     RLGFSPEAVRALNPKIVYCAALGYGSDGPYSGRPAYDDIMQCSAGLAGLALMRDGEPS
                     YHPTVTADKVAGLHVVYAVMAGLLHRERTGEGLNVEMPMFEALAAFSMNEHLMGATFE
                     AEGSLGYGRTMSRNRRPFKTSDGWIGVLPYTREQWTRVLKMLGREDVIAEPWFPDNGK
                     RNARSGELYQLLIDALPGRTSKEWLAAFEAADVPCGPMNSARELLEDPHLKAVGFFDP
                     HFEGNAPIKRTLRQPVLFRDMAAEPDRAPPALGADTAVLLAELGYGAAELADLTAKRA
                     IAGQPS"
     gene            8546..>9864
                     /locus_tag="PK264_11350"
     CDS             8546..>9864
                     /locus_tag="PK264_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRD76518.1"
                     /translation="MTTHDPDKAVYDLTTFGRVIVLISVILSSTCYTATILVATTLLP
                     QMQGGLSATQDEISWVMTFNILATAVATPMTGWLVSRLGRRRTMMWCLLGFTATTFMC
                     GASASLESLVFWRVMQGALGAPLIPLSQTLILDSFPRRQHSFVISLFGMSNIIGPVIG
                     PVFGGYIGEVLGWQWGFFMVLPVSMLAYTLFRFFLPADPGEATPAPLDWIGFLSLSTA
                     IASIQLLLSRGQRLDWYESTEIWIETGTAVIAFYIYLTHSLTTDRPYLNLSLFRDRNY
                     AIGCIFVTLYGMLHFAPVVLLPSLLQQHAGFPDAAIGAFVGWRGVGNGLGFFSAMWMQ
                     RLDPRISLLMGATIQTLSGYWLMHINLNVDQTTLALNSLMQGFSVGILWVPLTVATFA
                     TLDPKYRPEGSSVFHLLRNLGSSLFISIAVAEIIRTTGANYSRMTEFV"
     assembly_gap    9865..9874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9875..10083
                     /locus_tag="PK264_11355"
     CDS             <9875..10083
                     /locus_tag="PK264_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76519.1"
                     /translation="NRALQLPWVTGAWTTETLTGLARISREINRQATMIGFSNAFLLY
                     TALSAVAIPLSFLIQKQRATAAKA"
     gene            complement(10099..10812)
                     /locus_tag="PK264_11360"
     CDS             complement(10099..10812)
                     /locus_tag="PK264_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD76520.1"
                     /translation="MSTLFSWGDLPGHIAYVILAISYWLTSMFWLRTLACIGLFFEIA
                     YYTSVGSRMWVGIAWDLVFISINLFQLYLIFKDRFSLKLAEAEGAVVRRAFGGLDDAQ
                     LARLLRAGSWSSFEPGTELTTAASPVNALSLIQSGRAEVIVDGHTIACLRPGDFIGEI
                     AFLTGVNATATVKATEPLRALVLERAKLAKLCASDGEIATAVYQILGSGLAQKFAVTH
                     DKLISLVPPPAPSSAPAKA"
     gene            11020..>11393
                     /locus_tag="PK264_11365"
     CDS             11020..>11393
                     /locus_tag="PK264_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76521.1"
                     /translation="MLVRAVLMSLLVMAGSPGSARAQEIAGSAYVMSQSPAGVCPATV
                     SIEGTIRSNMAGQIRYQWVRSDGTESPEETAFLNEPGSVSVMHHWRLAPTAGRFAGWV
                     KMRLSFENVSAESEPVSVTVQCA"
     assembly_gap    11394..11421
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11422..12473
                     /locus_tag="PK264_11370"
     CDS             <11422..12473
                     /locus_tag="PK264_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76522.1"
                     /translation="ASHAGTCPTRLTFDGQIRSDQPGNVRYVWVRSDGVISPEATVTF
                     AGPGVQPVRTTWELGTPGFSGQVSMVLRVLTRGRAISSTPAAAVLRCAAPPPVALPPK
                     VSPEVRVALFVSPVRHKGPCPVTIKFEAVIKSNVAGPVRYVWERSDGARSPERTVTFA
                     APGEQVVAEKWSLGAANVKTKAGQVLRILSPVANVSATFAAIVECDGPAVTPPPVTPP
                     RVTPPPVTPPPVLPKAAVTVSASVEPARHDGACPVTVRFTGVIGSNRAGPVRYRWISG
                     GFRDNRTETGPDQTITFDAPGSRTVTDTWAHERRFKGWRKLELLAPATATSAEAAFEV
                     ICSGDDAKKKRKPKE"
     gene            12609..13235
                     /locus_tag="PK264_11375"
     CDS             12609..13235
                     /locus_tag="PK264_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76523.1"
                     /translation="MRLFNLSISLITFGGSAYALIRADRIPGWAKGVAIAGVVISGLH
                     LAMTLVIQSPEIGHGISYIRTQLTGRLGEPVRQDPSWTRTATPPVAVAYKPCVALAMS
                     LTGQWAVDGGASGCEAHGATAARACAQKAGGPCSTHASGGNWVAGVTCAVQTVKGIYS
                     RSFASNGDSEEDAYARAFASAERQGLQRPFCRPAIASPLDASLARQPR"
     gene            13326..14006
                     /locus_tag="PK264_11380"
     CDS             13326..14006
                     /locus_tag="PK264_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRD76524.1"
                     /translation="MKLVIFDCDGTLVDSQNAIFAAMQHAFGREGLPVPPRHRVLEVV
                     GLSLPEAFAVLAGEHARPVQTRLAEAYRTDFPRAREEVGERDPLFEGTAELIAALFGA
                     GDVMLGIATGKSRRGVARLLAQEGWEGHFATIQTADTHPSKPHPSMIETAMRETGVGP
                     ERTVMIGDTSFDMEMARAAGVGAIGVSWGYHPVERLESAGAHRIVAAWPDLHRAIAGC
                     LDAAGARR"
BASE COUNT         2289 a         4493 c         4634 g         2323 t
ORIGIN      
        1 ctccggacaa gacgagcccg gaccggacca gcctgccgcc tggctgaatc ggctctcgcc
       61 gccccaatct ccgatggcat tcaacgggtt ccctatacag gggccatgcc cctggagatt
      121 ggccgcctgc catgcgtgcg tcgctcacag ttcctgagat cgtggtcgca ggctggctgc
      181 tggcgggtct tttcctgctg cttctcggtg gcatggacat tgttcgacgt ggcaatgacg
      241 acccgtcagg gcgtactccg gcctattggc tcccnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnncatc tggtacgtcg tcggcacgac gcccatgtcc ggcccgagtg cggcaccgac
      361 acgcaagagc gagggtcaac cgggcgccac ccggtatagg cgtccgagac catcaagtgg
      421 ctcctgaatg ccggccagtc gcagcatgtg ccagatcagg gcgtggttac tagacgtggc
      481 cggcatgcct atcgcctgct cgatcgcgcc gatcacatcg agtgtccgca ggctcgtgca
      541 cgacacaaac acgctgtcga catcgctggc ggcgccgaca cgccgcgctg tgtcggcaat
      601 cgcctgctgc gtgatgcggg caacgacgcc gtcgtccggt tcctcaaagg aggcgagcgc
      661 cggcacttcg atgccgcgtg ccgcaaggcc ggagatgatc gtgtcgttga caggacgggc
      721 atacggcgtg atcagggccg gccggcgaac gcccaaggcc cgcaaagccg cgatgactgc
      781 cgttaccgga tcggtgaccg cggcggcgct atgcaccgat ctcacgagcc gcgcgacctc
      841 ggcctcgccg atcacgatcg cgccggacgt gcaggcgaag ccgatcgatt tgaagctcca
      901 ttccgctggt aacagccgtg ccgtatccgc aatgagcggc ttcatggcga gcagcgtctc
      961 gcgggtgatc tccgtgtcgt tccaaagccg ggccgcatag acggcaacgt ccggcagccg
     1021 ctgaatgacc tgtcggaggt cgctctcgaa cgtctggtcg gtgcgcagca cgaggagccc
     1081 gatccgcgcg cgagccccga ggccggcgtc agtgtgcagc ggcacacgct cgtagcgcat
     1141 ggttccctct cgaagacctg cagcagcacc cgtgcggaca tcatgcgtgg tgcagtcacg
     1201 gagggcaagg ctgacgacaa gcgatggaag gagtgcggat ggctcggtgg ctctatgggg
     1261 gccgcgaggt tgcatatgag cgcctgacgc gcacgtgact tgccggtgat cccgcttcgc
     1321 tctagctagg tttcgggcta ggcgtacctt gaaatcgcga tcggtggacc ttcatgcttg
     1381 gcgaactctg gctctatgcg ggcgtcatgc ttgccggctt ggtgagcttc ctttcgcctt
     1441 gtgttctgcc gctagtgccg ccgtatctcg gctacatcgg tggcaccacc atcgagcaga
     1501 tgaccgccga tcatggcctt gaccgctcgg cgtcgcgcaa ggtcatcatc gcggcattgg
     1561 tgttcgttct cgggttcacg acagtattcg tttcgctcgg cgcggccgcg tccatggccg
     1621 gacaattcct cgcgacgtgg aagcgagagc tcggctgggt cgccggcggc gtgatcatcc
     1681 tgtttggcct gcatttcctc gggattttca agatcccgct cctatatcgc gaggcgcgct
     1741 atcatggccg cgtggagggg gcgagcctcg ccggcgccta tctcatgggc ctcgcgttcg
     1801 ctttcggctg gacgccttgc atcggcccgg tcctgtcggc ggtcctcggt ctcgccatga
     1861 gcgaaaacag ccttggagca ggcgtcaagc tgcttttcgc ctactcgctc ggcctcggaa
     1921 taccgttcgt gctcgcggcc gtggcgatcc ggccattcct cggcttcatg ggccgattca
     1981 ggcgccatct cggtcgggtg gaaaagatca tgggggcgct gcttgtcctc accggcgtcc
     2041 tgttcatctt cggcggcatc ggctggatgg ggcagtggct catagacaac gtgccgattc
     2101 tcgcgcgcat cgaggagatg ttcacctccg acaagctgcg catcgagatc atgaagaagg
     2161 gcgccgagaa gtaggcgcga acgcaagggc tgcgcatgac caccgcccgc attccgaccg
     2221 ctctcacgat cgccggatcg gacagctccg gcggcgccgg catccaggcc gatctcaaga
     2281 cctttacggc gctcgggatc tatggcgcct cggtcatcac ggcgctcacc gcgcagaaca
     2341 ccttgggcgt gcaggccgtg gcggctgtcg agccgggatt cgtgcgacga cagatcgcct
     2401 cggtgatgga cgacctcgac gtccgcgcca tcaagacggg gatgctcgcc aacgccggcg
     2461 tggttgcggc ggtcgtcgat gggctcgcgg cgaggcagac gaaccgcaat gccatcccgc
     2521 tcgtcgtcga tccggtcatg gttgcgacca gcggcgacag cctgctccag gccgacgcga
     2581 tcggcatcat cacacggcgg ctattcccgc tcgcgagcgt cgtcaccccg aaccttccag
     2641 aggcggcacg gctcctcggc acaagcgagg cccgcaccac ggacgaggcc atcgaccagg
     2701 ccagggccat cctgcagctc ggccccggcg ccgtgctcgt caaggggggc catggcaggg
     2761 gcagcgaggc ggtcgatgtg ctcgtcaatc actccggaat cgagctgctc accgccgagc
     2821 gcatcgccac gcacaacaca cacggcaccg gctgcaccct ctccgccgcg ctcgctgcag
     2881 gccttgccga cgggctaggt ctgatcgacg cctgccggtg cgccaaggcg ttcgtcagcg
     2941 atgccttgag atccggcgcc gtccgccccg tcgggcacgg gcacgggccg gttgatcaca
     3001 tcttcgcaat ccgcaatcgc aggcggcaat agctctgcaa gctgcacctt gttgctgcgc
     3061 cgccacggtc gtgtcgcaat atccggcgcc ctgttgcgct cacctgggca atcggccaag
     3121 ttgatccggc gaacacgctc cggggggatg agtggcatcg tgacggtggc gaaagccact
     3181 tggtatcgtc agtcggggga cgcgcgatac cagactgacc ggctgtcggg ggacggccgg
     3241 tgttgctgag agtgcctcga gggaggcgcg ctttcagatc caaagggccg gtccggttta
     3301 tccgggccgg ccctttcgat tcctacctcc gatcctgcag cggcgaggca tccaatcaac
     3361 ccttgtcctt tagcgtagaa tagggccgga cctgggcccc gcgcgcctct gtgcccaagc
     3421 agcagaccgg cctggattgc atcggaccag gcgttcccgg gctggtaagg tcaaggtcgg
     3481 cacgtcgcag cgtcgtcgtg tgacgtgggt tgacgtctgg tcagctgaag cggcttgcca
     3541 atcccgaggc ttggtaccta ctgtcgggcg cctgcaactg cggcgaggag cgacgcatgg
     3601 ccctgacctt cagatgctcg gcgcgctccg gcgccgaaac gcgctccttc gagccgaccg
     3661 cgctcgtcgt tgccggctgg acagggcgtg atgagaaaag tctccagcat catatcgagg
     3721 agctggccgc gatcggcgtg ccccggccct cgtcggtgcc ggtgttctat cgcaatgcca
     3781 tagccaacgt gctgcaggct gaccgcatcg aagtgctcgg cccggatacc tctggcgagg
     3841 tcgagccggt gatcctcgcc atgcctgatg ggctttggct cacggtgggc tccgaccaca
     3901 ccgaccgcaa agccgagacg atgggcattg cgctttcaaa gcaactctgc ccgaaagtgc
     3961 ttggccgcga ggcgtggcgg ctcgacgaaa tcgagacgca tttcgacgag atcaggatcc
     4021 gcgcgttcgc ggtcatcggc ggcaagcgcg tcgcctacca ggacgggacg ctcgcgacgt
     4081 tgcgggcgcc gtccgacctc atccagcgct acggcaaggg gaagccgctc gcgccgggca
     4141 cggtgatgat gggcgggacg ttcggcgcga tcggcggcat caggccggcg gatcgcttcg
     4201 agatggagct cgatgacccg agaactggga gggtcatccg cgcggcctac gctatcgaga
     4261 cacttcccgt cgtgacgtga cgaccgacaa tcgagagcag gagcatgacc gccgcaatgc
     4321 caacaatcgc cgacgtgcgc gccgcacgag cgcgcctcgc gccgcatatc atgccgactc
     4381 cgcttatcga acatcccgcg ttgaacgcgc tcgccaaggg ccgcgtgctg ctcaaatgcg
     4441 agaccatgat gcgtgtcggc gccttcaagt tccgcggcgc ctacaatgcg gcctcgcagg
     4501 tcgacagggt acgattcccg ggcggcattg tcgcctgctc gtcgggcaat cacgcgcaag
     4561 gagcggcggc agcagcgaca ctactcggca tgcactcggc gatcgtcatg ccggcggacg
     4621 cgcccgcgct gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cggcctcgag atcatggagg
     4801 aggcgaaggc gcgggggctg gtgccggacg cagtgctcgc cccttgctcg ggcggcggcc
     4861 tcatcggcgg gattgccatc gcggtgaagg acgcgagccc gaagacggag gtgatgagtg
     4921 tcgagcccgc gggcttcgat gaccttgccc gctcgctcgc ctcgggaaag cctgagaaga
     4981 acgccaagct ctccggctcc atctgcgacg cgctgctggc gccgtcgccg ggctcggtga
     5041 cgtttccggt ggcgaaggcg ctgctcacgc gaggcctcgt cgtcacggat gatgaggcgc
     5101 gtgtggcgat ccgcttcgcc ttccacgagc tgaaactggt gctcgagccg ggtggagcag
     5161 tggcgctcgc cgcggtgctc gcgaggaagc tgccgaccga aggccgcacc gtcgcggtcg
     5221 tgctctcggg cggaaatatc gatccggaga tgtttgcgga gatcatccgg aatgacgaca
     5281 agctcgatag ctgagcaggc cccactcctt gcttccctcc ccccttgtgg gggagggacc
     5341 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnccgg tcctgcacca cccccatccg gtcctgcacc
     5461 acccccatcc cctgcccttc ccccgtctag ggggaaggga gcagttttct gccccatgac
     5521 ctagcgtcca agtatcagct accattcgtg acgcgtggcc accgcgacga ggagaagcga
     5581 aatgacggtc gagatgaagc gcgtgctggt tacgggggct gcgggcggcg tttgcacgtg
     5641 gctccggaag ctgctgccgg aggtctaccc cgacatcgtc tggtcggatc gggtgaagcc
     5701 gaaggatctc gcgtcgaacg agacattcat ccaggccgag ctttcgaagc ccgccgagat
     5761 cgaagcggcg tgcaagggca tcgacggcat cgtgcatctc ggcggctact cggtcgaggg
     5821 cccatgggag acgatcctcg aggcgaatat cgttggttgc tacaatctct tcgaggcggc
     5881 gcggaaggcc ggcgtgaagc gcgtgatctt cgcctcgtcg aaccacgcca tgggcttcca
     5941 cccgcgcgcg aggcctatcg acacggacgt gacgggccgg ccggacagcc gctatggcgt
     6001 ctcgaagctc ttcggcgaag ggctcggcgc catgtatgcc tacaagcacg gcatcggcgt
     6061 cacctgcctc aggatcggca atgtcgctga ccagccgatc gacgtccgcc ggctgtcgat
     6121 ctggctcaag ccctccgacc ttgtggagct gatccgtatc gggctcgagc ggccaggcat
     6181 cgtcttcgag gtgatgtacg gcgtctccga caacgaggcg agctggtacg acaacgagcg
     6241 cgcgttccgg ctcggctatc ggccgaaggg acgggccgag gatttccgcg cggcggccga
     6301 agcggcacag aagaagattg cggccgatcc ggtcgccgac ttctaccagg gcggcagctt
     6361 ctgctcggcc gagtattccg ccgatttcga ggacctgaag aacaagagct gaggcgtcgg
     6421 gtccagcgga gacgggaggc gaaacatgcg tggactgaag ggcaaggccg tgnnnnnnnn
     6481 nnnnnnnnnn nnnnnggcgg ggcgattgcc ggccggctcg cggaagaagg cgcgatcgtc
     6541 ggcatcttcg acaagaacgg ctattccgcg gaggctgccg cggagaagct cggccagggt
     6601 tcgatcgcgg tcgcctgtga catcacggat cacgcctggg tcgtgaaggc ggttgcagaa
     6661 ttcgaggcga aggctgggcc aacgcacgcc ctcgtcaaca acgcgggctg ggacaaattc
     6721 gccttcttcc tagacaccaa acccgagctc tgggaccagc tcatcgccat caatctgaag
     6781 ggcccgatca acatgcacca tgcggtgctg aagggcatgg cgcagcgccg cgctggccgc
     6841 gtcgtcaaca tcgcctctga tgcgggccgc gtcggctcgt cgatggaggc cgtctattcg
     6901 gcgtgcaagg gcggcatcat ctcgttcacg aagaccatgg cgcgcgagat ggcgcgctca
     6961 ggcgtgacac tcaatgcggt ctgccccggc ccgaccgaca cgccgctgct cgccgacttc
     7021 atgggcgaag gcgagaaggg caagaagatc cacgatgcgc tcgtctcggc gatcccgatg
     7081 aagcggctcg gccggccgga ggatctcgcc ggcatcgtca cgttcctggc gagcgacgag
     7141 gcggcctaca taaccgggca ggtgatcagc gcctcgggcg gtctcacgat ggtcggctga
     7201 gcaagcgacg acagagccag aagagcgagc gcgatgtacc cgatcctcaa aggctaccgc
     7261 atcgtcgacg tgacgacgat cgtgctcggc ccctacggca cgcagattct cggcgatctc
     7321 ggcgcggaag tcatcaaggt cgaggcgatc gagggtgacc tcaatcgctg gacgctgccg
     7381 gcggcggaac cgggcgtcgg cgctgtcttc gccaacaaca accgcaacaa gcgatcgctc
     7441 gccgtcgacc tgaagcagga tgccgggaag gaggttctgc gtcgactggt cggcacgtcg
     7501 cacgctttcg tccacaacat gcgcgcctcg gcgattgagc ggctcgggtt cagtccggag
     7561 gccgtgcgcg cgctgaaccc caagatcgtc tattgcgcgg ccctcggata cggcagcgat
     7621 ggcccctact cgggtaggcc agcctacgac gacatcatgc agtgctcggc gggcctcgcg
     7681 gggctcgcgc tcatgcgcga cggcgagccg agttatcacc cgacggtgac ggccgacaag
     7741 gtcgcggggc tgcacgtcgt ctacgccgtg atggctggcc tcctgcatcg cgagcgcacg
     7801 ggcgaaggcc tcaacgtcga gatgccaatg ttcgaggctc tcgcggcgtt ctcgatgaac
     7861 gagcacctga tgggtgccac cttcgaggcg gaaggctcgc tcggctacgg acgcacgatg
     7921 tcgcggaacc gccggccgtt caagacctcc gacggctgga tcggcgtgct gccctatacg
     7981 cgcgagcagt ggacgcgcgt cctcaagatg ctcggccgcg aggacgtgat cgcggagccg
     8041 tggtttccgg acaacggcaa gcgcaacgcg cgttcgggcg aactctacca gctgctcatc
     8101 gacgcgctgc ccggccgcac gagcaaggag tggctcgccg ccttcgaggc agcggacgtg
     8161 ccgtgcgggc caatgaattc ggcgcgcgag ctgctcgagg acccgcacct gaaagccgtc
     8221 ggcttctttg acccgcattt cgaaggcaac gcgccgatca agcggacgct gcgccagccc
     8281 gtgctgttcc gtgacatggc ggccgagccg gacagggcgc cgcccgcgct cggggccgac
     8341 acggccgtcc tcctcgcgga actcggctac ggcgcggcgg agctcgccga cctcacggcg
     8401 aagcgtgcga tcgccggaca accgagctga aggccacgcc tgaggattgc gcacagccgc
     8461 cctgcagctg gcgcgtgcgg tggcgggcgc gcggcggctg cgggcctgct aagcctcagg
     8521 gaccgaccat gcgcgagaca cgccggtgac gacccacgac ccggacaagg ccgtctacga
     8581 tctcacgacc ttcgggcgcg tgatcgtgct catctcggtg atcctgtcgt cgacctgcta
     8641 tacggcgacc atcctcgtcg cgacgacgct gctgccgcag atgcagggcg gcctctcggc
     8701 gacgcaggac gagatctcgt gggtgatgac tttcaacatc ctggcgacgg cggtggcgac
     8761 ccccatgacg ggctggctcg tctcgcgact cggccggcga cgcaccatga tgtggtgcct
     8821 gctcggcttc acggcgacga ccttcatgtg cggcgcctcg gcgtcgctcg agagcctcgt
     8881 cttctggcgc gtgatgcaag gcgcgctcgg cgccccgctc atcccgctca gccagacgct
     8941 gatcctcgac agcttcccgc gccggcagca ctcgttcgtc atttcgctgt tcggcatgtc
     9001 gaacatcatc gggccggtga tcggcccggt gttcggcggc tacatcggcg aggtgctcgg
     9061 ctggcagtgg ggcttcttca tggtgctccc ggtcagcatg ctggcctaca cgctcttccg
     9121 cttcttcctg ccggccgacc ccggggaggc gacgcccgcg ccgctcgact ggatcggctt
     9181 cctttcgctc tcgacggcga tcgcctccat ccagcttctg ctctcgcgcg ggcagcgcct
     9241 cgactggtat gaatcgaccg agatctggat cgagaccggc accgcggtga tcgcgttcta
     9301 catctacctg acccacagcc tgaccaccga ccggccgtac ctaaacctga gcctcttccg
     9361 cgatcgcaat tacgcgatcg gctgcatttt cgtgacactc tacggcatgc tccacttcgc
     9421 gcctgtggtg ctgctgccct cgctcctcca gcagcacgcc ggattccccg atgccgcgat
     9481 cggtgccttc gtcggctggc ggggtgtcgg caacgggctc ggcttcttct cggccatgtg
     9541 gatgcagcgg ctcgacccgc ggataagcct gctgatgggc gcgaccatcc agacgctctc
     9601 cggttactgg ctgatgcaca tcaacctcaa cgtcgaccag acgacgctcg cattgaacag
     9661 cctgatgcag ggcttctcgg tcggcatact ctgggtgccg ctgacggtcg cgacgttcgc
     9721 gacgctcgac ccgaagtatc gcccggaggg ctcgagcgtg ttccatctcc tgcgcaacct
     9781 cggctcgagc ctcttcatct cgatcgccgt cgccgaaatc atccgcacga ccggtgccaa
     9841 ctactcgcgc atgaccgagt tcgtnnnnnn nnnnacaatc gtgcgctcca gctcccatgg
     9901 gtgacgggcg catggaccac ggagacgctt accgggctcg cccgcatctc gcgtgagatc
     9961 aaccgccagg cgaccatgat cggcttctcg aacgccttcc ttctctatac ggcgctgtcg
    10021 gcggtcgcca ttccgctgag tttcctcatc cagaagcagc gggcgacagc cgccaaggcg
    10081 tgacgaggcg atcctcgctc aggccttggc cggagcggag cttggcgccg gcggggggac
    10141 gaggctgatc agcttgtcgt gcgtcacggc gaacttctgg gcgaggccgc tgccgagtat
    10201 ctggtagacg gcagtcgcga tctcgccgtc gctggcacag agcttggcga gctttgcccg
    10261 ctcgaggaca agagcacgaa gcggctcggt cgccttcacc gtcgcggtcg cgttgactcc
    10321 ggtcaggaag gcgatctcgc cgatgaagtc gccggggcgg aggcaggcga tggtgtggcc
    10381 atcgacgatc acctctgccc gcccggactg gatgagcgag agggcattga cggggctcgc
    10441 ggccgtggtg agctcggtgc cgggctcgaa agacgaccat gatccggcgc ggagcaggcg
    10501 ggcgagctgc gcgtcgtcga gcccaccgaa ggcacgccgg accacggctc cttccgcctc
    10561 ggcgagtttc agcgagaagc ggtccttgaa gatcaggtaa agctggaaga ggttgatcga
    10621 gatgaagacc aggtcccagg cgatgccgac ccacatgcgc gagccgacgg acgtgtaata
    10681 ggcgatctcg aagaacagcc cgatgcaggc gagcgtacgc agccagaaca tgctggtgag
    10741 ccaataggat atggcgagta tgacgtaggc gatgtgcccg ggcaaatcgc cccagctgaa
    10801 tagcgtgctc atatgtcgtc tctcccaacc cgcacccctc tcgaaaagac aatgccacag
    10861 tcgcctggct gcgcctgtgc ccggaagcac acgatttggg aagcctgcgg tctcgggatt
    10921 tcgagcaccg ccggcctggc tcggcgtcga cggacgattc tttttgcgct caagtccgga
    10981 caccgcctaa tgtcgcaaac gatgccaagg gagatcgcga tgctggttcg agcggtgctg
    11041 atgtcgctgt tggtgatggc cggatccccg gggagtgcga gagcacagga aatcgccggg
    11101 tctgcctacg tgatgtcgca aagcccggcc ggggtttgcc cggcgaccgt gtcgatcgaa
    11161 gggacgatcc ggtcgaacat ggccggtcag atccgctacc agtgggtgcg atcggacggg
    11221 acggaaagcc cggaggaaac agcgttcctc aacgagcccg gatcggtcag cgtgatgcat
    11281 cattggcgcc ttgcgccaac tgcaggccgc ttcgccggtt gggtgaagat gcggctgtcg
    11341 ttcgagaatg tttcggccga gtccgagccg gtctcggtga cagtccagtg cgcnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn ncggcgagcc atgccggcac gtgcccgacg cgcctcacat
    11461 tcgacggcca gatccgctcg gaccagccag ggaacgtccg ctatgtctgg gtgaggtcgg
    11521 acggcgtcat ctcaccggag gcgacggtca cgtttgcagg gccgggcgta cagccggtgc
    11581 gcacgacctg ggagctcggt acgcccgggt tcagcgggca ggtctcgatg gtgctgcgcg
    11641 tgctcacgcg cgggcgcgcg atcagctcga cgcccgctgc ggcggttttg cggtgcgcgg
    11701 ccccgccacc ggtcgcgctg ccgccgaagg tgtcgcccga ggtgagggtc gccctgttcg
    11761 tgagccctgt ccgacacaag gggccatgcc ccgtgacgat caagttcgag gccgtgatca
    11821 agagtaacgt cgccggcccg gtccggtatg tgtgggagcg cagcgacggc gcgcgctctc
    11881 cggagcgcac cgtcacgttc gcggcgccgg gcgagcaggt cgtggccgaa aagtggtcgc
    11941 tcggggcggc caacgtaaag acgaaggccg ggcaagtgct ccgaatcctg agccccgttg
    12001 ccaacgtctc agcgaccttt gcggccatcg tcgaatgcga cgggcctgcc gtgactccgc
    12061 caccggtcac tccaccacgc gtgacgccgc cgccggtgac gccgccacct gtgctgccga
    12121 aagctgcagt gaccgtgtct gcgtcggttg agccggcacg gcacgacggt gcatgtcccg
    12181 tcacggtgcg attcacagga gtgatcggat caaaccgagc agggccagtg cgctaccgct
    12241 ggatctcggg cggcttccgc gacaaccgaa cggagaccgg acccgaccag acgatcacgt
    12301 ttgatgcgcc tggctcgcgg acggttaccg acacatgggc ccacgagcgg cgcttcaagg
    12361 gttggcgcaa gctcgagttg ctggcacccg caacggcgac gtccgcggaa gccgcgttcg
    12421 aggtgatctg ttcaggcgat gacgcgaaga agaagcgcaa gccgaaggaa taggcggatc
    12481 ggatcgtgcg cgcctgcgtc aaccggcgct cggtgcggcg ggcggccctt gaccttgggg
    12541 cgcgcatgcc taaatccgac tatcgggtcg atcgggcttg gcgatcgccg ccgacgtcat
    12601 gagggatcat gcgtcttttc aatttgagca tcagcctcat cacgttcgga ggcagcgcgt
    12661 acgccctcat cagggcggac cggattcccg gctgggcgaa aggtgtggcg atcgcgggcg
    12721 tggtgatctc ggggctgcat ctcgcgatga cactcgtgat ccaatcgccg gagatcggcc
    12781 acggcatcag ctacattcgg acacagctca cgggacgcct cggcgagccg gtccgccagg
    12841 acccgtcgtg gacgcgcacc gccacgccgc cggttgcggt tgcctacaag ccgtgcgtcg
    12901 cactcgcgat gtcgcttacc ggacagtggg cggtcgatgg cggcgcatcg ggctgtgagg
    12961 cgcacggggc gaccgccgcc agggcatgtg cccagaaggc cggcgggccc tgctccacgc
    13021 atgcgagcgg ggggaactgg gtcgcgggcg tcacgtgcgc ggtccagacc gtgaagggca
    13081 tctactcgcg ctcgttcgcg agcaatggcg acagcgagga ggacgcctat gcgcgcgcct
    13141 ttgcgagcgc agaacggcaa ggcctgcagc ggccattctg ccgcccggcc atcgcctcgc
    13201 cgctcgatgc ctcgcttgcg cgacagccgc gttaggtcca ggagagtgcg gtcgcaagac
    13261 atgaccgcgg ctggtcgctg gcagcgtgcc aagcaccgga gtcacccgag gagcagaaat
    13321 ctcccatgaa gctcgtgatc ttcgattgcg atggcacgct ggtcgacagc cagaacgcga
    13381 tattcgccgc catgcagcac gcattcggcc gggaggggct gccggtgccg ccccgccacc
    13441 gcgtgctgga agtcgtcggc ctctcgctgc ccgaggcgtt tgccgtgctt gcgggcgagc
    13501 acgcccggcc cgtgcagacc cggctcgcag aagcctatcg caccgatttt ccccgggcgc
    13561 gtgaagaagt cggcgagcga gatccgctgt tcgaggggac cgccgagctg atcgccgcac
    13621 tcttcggtgc aggtgacgtg atgctcggca tcgccaccgg aaagtcgcgg cgcggcgttg
    13681 cgcgcctttt ggcacaggaa ggctgggaag ggcacttcgc caccatccag acggccgaca
    13741 cgcacccctc gaagccgcat ccctccatga tcgagacggc gatgcgcgag acgggggttg
    13801 gacctgagcg caccgtcatg atcggcgata cgtcgttcga catggagatg gcgcgggcgg
    13861 caggcgttgg cgcgatcggc gtcagctggg gttatcaccc ggtcgagcgg ctcgagagtg
    13921 ccggcgcgca tcgcattgtc gcggcatggc cggaccttca tcgcgcgatc gccggctgcc
    13981 tcgacgctgc aggggcgcgc cgatgagcgg cgcgaaggag gctcaaccga cacgcaagcg
    14041 cttctacaag caggcggggt tcg
//
LOCUS       DAORNH010000096        13893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3333_length_13893_cov_2.661295, whole genome shotgun sequence.
ACCESSION   DAORNH010000096 DAORNH010000000
VERSION     DAORNH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13893
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3333_length_13893_cov_2.661295"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PK264_11385"
     CDS             <1..768
                     /locus_tag="PK264_11385"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD76525.1"
                     /translation="EAVRLYRLAADKGVAEAMNSLGLMYAKGHGGLAKDEVEAVRLYR
                     LAADKGVAEAMSNLGAMYANGRGGLAKDEVEAVRLYRLAANEGIAEAIHNLAWMYANG
                     HGGLTKDEVEAVRLFRLAADQGIAEALYSLGAMYWDGRGQLSKDEAIAAQFYRLAAEK
                     GDSAAMHNLGVLYALGRGGLNRDVRLAARWLVAAIVAGNQVTIDQSMGSVDPFGADLR
                     RAIIERLKSEGHYSGPVTDTFTPAVFAAMERLVARAK"
     gene            complement(1199..1987)
                     /locus_tag="PK264_11390"
     CDS             complement(1199..1987)
                     /locus_tag="PK264_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRD76526.1"
                     /translation="MRKNKVREIVGKGGRIINGWLAIPSSYSAELIAHSGFDSVTIDL
                     QHGPVDFTSAVPMLQAISTTDSVPMARVPWNEPGIIMKMLDAGAYGIICPMINTKEEA
                     EAFASYGRYAPRGQRSMGPNRAVQYGGADYAVNANDEILLFAMIETTKALANLDAILS
                     VPGIDGVYVGPSDLSISLGKPATLDPSDKEVLAAIATICKKTRAKGLIAGVHTDGVKT
                     ATKRFDEGFQLCTILNDARLMAMAAQQAVRELKGQAPAAAAKTY"
     gene            complement(2149..3468)
                     /locus_tag="PK264_11395"
     CDS             complement(2149..3468)
                     /locus_tag="PK264_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD76527.1"
                     /translation="MKTTSRYALAAAALMASASMANAADLGGNCCADLEERVAELEAT
                     TVRKGNRKVSLTISGFVAHNVMFWDDGTQSDMYIADGGNYGSRFRFVGTAKITPNVTA
                     GFLYEFGIANNAVNAVNQLNGGDDLGGSVALRDTTVWLRHSQLGMIKVGHGSTATDNL
                     VLIDLGGLGGAATMDASLYNGGFILRGPGGLASSTATNWAAAMRGHESFDTSRRNHVL
                     YETPSLHGFTLQAAVAEDNYWDVALRYAGEHHGFRVAFGIGYSEDTEFNAPNQLLNVA
                     GALCTTNCDTKTTDLKGALSILHVSSGLFVTTAYAKRELEGGLPAPGAYVGPDITNWY
                     IAGGIRQNWTGLGATVLFAEYGEFKGGLAQSAHLGLIATHDTVSNNTVDMWGLGILQN
                     VDAAAMELFLVYKNFSLDATGFNAANAHLNTSASDFSTVIIGTRIFF"
     assembly_gap    4054..4218
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4219..4902)
                     /locus_tag="PK264_11400"
     CDS             complement(<4219..4902)
                     /locus_tag="PK264_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76528.1"
                     /translation="MALSNFKEGMIVEIDPRTMPKAAGPQYVRELQALGARVSIYLVG
                     GHCDLGRDCASLGPDAVLETTGSYNWDKSERRVINILSAASRRRLEAGIENGWRIGAN
                     YIRIDNLHWPAGSGHPRDATVMKTIVDWAHDIEDRLRRDGVIPADRPTGVVAHNNLIV
                     WKELIERKRIRRLPAFLTSERSAQLYPARTDAKGRRLYEGDLKLQRGTLSPTELTEIK
                     AGGEIALAYG"
     gene            5373..5807
                     /locus_tag="PK264_11405"
     CDS             5373..5807
                     /locus_tag="PK264_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD76529.1"
                     /translation="MTVVTRKSYAFEDLQLGMEASVVRNVSESDIVTFAEISGDKNPL
                     HLDAAFAAKTMFKQRIAHGMLSASYISAVFGMEMPGPGAVYISQTLNFKAPVHIGARV
                     VAKVKVVELIAAKKRARFECVCLVDGKPILEGEAVLMVPSRA"
     gene            5916..6872
                     /locus_tag="PK264_11410"
     CDS             5916..6872
                     /locus_tag="PK264_11410"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRD76530.1"
                     /translation="MLKVIHGFSSVPAEARGAVLAIGNFDGVHRGHQALIREAKRIAA
                     ATPAPGAPSGVIIFDPHPREHFHPDRPHFRLTALDRKLELFDAYGARIAVVLTFDASL
                     AGMTAEAFVSRVLVEGLAVRHVIVGYDFHFGRNRGGDVRFLESAGLRNGFGVTVVPPV
                     AEGGEVFSSSAIRAELAQGDVESAAEALGHWWRVSGIVIGGAHLGTGFGFPTANIDLP
                     KCAGLAHGIYAVRVILADGRRFSGAAYLGTRPTVDDGPPKLETFLLDFSGNLYGQVIT
                     LEFVARIRGDKRFADFEELKAQMARDCEEARRRLAAAPETPV"
     gene            6960..8639
                     /locus_tag="PK264_11415"
     CDS             6960..8639
                     /locus_tag="PK264_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD76531.1"
                     /translation="MPNMQGKRAFLELLRQEGVDKIFGNPGTTELPLMDALAVETGID
                     YVLGLNESAVMAMADGYAQASGKVAVCNVHVAPGLGNAMGMLYDAKKAGSPVLVTAGQ
                     HDQAFTFQEPILWDDLATMARPLVKWSVEVTRLVDLPRAVHRAIKTALAPPMGPVFLS
                     LPVDVLQDTAEIDLGRPTRVARNLRGDPEAVAAAAKLLARAKSPVLIAGDAVAQSDAH
                     AEMVALAELLGAPVYAEGIPSRATFPSTHPLFQSNMQRLSPQIRAALEPHDVLFSVGG
                     DLFTQSLPTKADPVPPTTAIIHLDTDPWELGKNHPEEIAILGDPKSTLPDIIAALKAE
                     LGAEGLKRGAVRLDAAKKSAATRLAALHKEADALASATPVKPLALMKAIGDLVPKDAV
                     IVEEALSSADRMRFLIPSLDSKSWYGLRGGGIGWGVPATIGIKLAQPSRPVVGIIGDG
                     SSMYAIQALWSAAHHRIGCAVFVILNNGSYRILKQRMNAMREYAAQADHYPAMDLVDP
                     AIDFVGLAASLGVKGARAGSVAEFRDIFKRAVAGDAPFLIDVAMDRAFKPV"
     gene            complement(8739..>9029)
                     /locus_tag="PK264_11420"
     CDS             complement(8739..>9029)
                     /locus_tag="PK264_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HRD76532.1"
                     /translation="QQRNGFSAGNDTFVWEREDIGTGLDRIRDFMVGDRLDFSSLFCG
                     RDAASSMLRVTDTAAGTIVSADMGDAGFVDVVLLEGVHLDLDALLSNACVIN"
     assembly_gap    9030..9053
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9054..9839)
                     /locus_tag="PK264_11425"
     CDS             complement(<9054..9839)
                     /locus_tag="PK264_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRD76533.1"
                     /translation="MATISGTSGNNVIVGTRSSDRINGLGGSDIIDGAAGNDTIDAGD
                     GDDQVVGSTGNDKVYGGNGNDILRGDAGNDLLDGGAGNDQLSGGEGSDELLGGDGDDI
                     LSGGNGVDWLYGGSGNDQLDGGEGNDFMYGEAGNDTLRGGNGNDRLEGGDGDDQLYGD
                     GGNDHLDGGAGRDRLFGGEGNDRMTGGAGNDHLDGQNGDDTLDGGDGDDFLNGGLGFD
                     LIYGGTGNDGIYAGGLNDIVFGGDGNDTIYGDGGDDRIAGGRGS"
     gene            complement(10025..12067)
                     /gene="uvrC"
                     /locus_tag="PK264_11430"
     CDS             complement(10025..12067)
                     /gene="uvrC"
                     /locus_tag="PK264_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRD76534.1"
                     /translation="MATASATGPEVIAAYLKTLPGSPGVYRMLNAEAEVIYVGKARSL
                     KARVSNYARLGGHSNRIARMIAATASMEFVTTRTESEALLLEANLIKRFRPRFNVLLR
                     DDKSFPFILIARDHAAPQILKHRGARSRKGDYFGPFASAGAVARAINTLQRAFLLRTC
                     NDSVYESRTRPCLLHQIKRCSAPCTGEISREDYDRLVAEAIRFLKGGSQDVRAQLQAM
                     MMEAADRLEFERAAALRNRLQALAHVTGDQAINPGNVEEADVFAAHQEGGQTCVQVFF
                     FRTGQNWGNRAYFPRADRSFAAGEVLESFIAQFYDDKPVPRLILVSEDFESRELMAEA
                     LGSKAERRVEIRVPERGTKSEIVEHALTNAREALGRKLAETSSQRRLLEALTERFSLP
                     RVPRRIEVFDNSHIMGTNAVGAMIVAGVDGFVKGQYRKFNIKSDATTPGDDYAMMREV
                     LTRRFKRLAELATADGGTERAADGDAHATAEAPARRDEVAPPASGNNSQGEGVDGADE
                     ASQSTEADLDADAALMEDLPERPDLVLIDGGPGQLAVAREVAAELGLADLTLIGVAKG
                     PDRDAGRELFHIPGRERSLMLEPRDPVLYFVQRLRDEAHRFAIGTHRARRAKALSANP
                     LDEIAGIGPTRKRALLGHFGSAKAVSRAGVADLQAVSGISAEMAQKVYDFFHDRGE"
     gene            complement(12156..12674)
                     /locus_tag="PK264_11435"
     CDS             complement(12156..12674)
                     /locus_tag="PK264_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRD76535.1"
                     /translation="MDLNGEYRIAAPRDVVWAALNDPDVLRQCIPGCETLEQKSPTEM
                     AARVRLKIGPVSAAFNGAVTLSNISAPESYTIAGQGQGGAAGFAKGHADVRLAEDGPE
                     ATVLSYAARADVGGKLATLGGRLITATSQKLADEFFGNFVKVVSGEDAADAGAAGGEK
                     KKGFWKRWLGKS"
     gene            complement(12764..13216)
                     /locus_tag="PK264_11440"
     CDS             complement(12764..13216)
                     /locus_tag="PK264_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD76536.1"
                     /translation="MAHGLYFEEFEIGRVFKHAITRTVTEMDNTLFCCLTHNPQPLHI
                     DHHFAAQTEWKRPLVNSLFTLGVVIGISVNDTTLGTTVANLGMSDVKFPKPVFHGDTL
                     RCETEVVARRESKSRPDAGIVEFVHRGFNQTGDLVAECRRQAFMRKRP"
     assembly_gap    13267..13296
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13587..>13893
                     /gene="ssb"
                     /locus_tag="PK264_11445"
     CDS             13587..>13893
                     /gene="ssb"
                     /locus_tag="PK264_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRD76537.1"
                     /translation="MAGSVNKVILVGNVGKDPEIRRTQDGRPIANLRIATSETWRDKN
                     SGERREKTEWHSVVVFNEGLCKVVEQYVKKGAKLYIEGQLQTRKWQDKDGQDRYSTEV
                     "
BASE COUNT         2330 a         4597 c         4397 g         2350 t
ORIGIN      
        1 gaggccgtgc ggctctatcg cctcgccgcc gacaagggcg ttgccgaggc gatgaacagc
       61 ctcggcttga tgtacgcgaa gggccacggc ggactcgcca aggacgaggt cgaggccgtg
      121 cggctctatc gcctcgccgc cgacaagggc gttgccgagg cgatgagcaa cctcggcgca
      181 atgtatgcga acggccgcgg cggacttgcc aaggacgagg tcgaggccgt gcggctctat
      241 cgacttgccg ccaacgaggg cattgctgag gcgattcaca acctcgcctg gatgtacgcg
      301 aacggccacg gcgggctcac caaggacgag gtcgaggccg tgcggctctt ccgcctcgcc
      361 gcagatcaag gcattgctga ggcactatac agcctcggcg caatgtactg ggacggtcgc
      421 ggccaacttt ccaaggacga ggcgatcgca gcgcagtttt atcggctggc cgccgaaaag
      481 ggcgatagtg cagccatgca caacctcggc gtcttgtatg cattgggccg tggtggcctg
      541 aaccgcgacg tgcgcttggc tgcgcggtgg ctggtagccg ctatcgtagc cggaaaccaa
      601 gtaaccatcg accagtcgat gggcagcgtc gacccctttg gtgccgacct ccgccgagcg
      661 atcatagagc ggctgaagtc cgaggggcac tactcgggac cggtcaccga caccttcacg
      721 ccggccgtgt ttgcggcaat ggagcggctc gtcgcgcgcg ccaagtgagg cggcgtcggt
      781 ggaaacgggt gtcaacccca aggatctgaa acccccgatc acgcatctgc agcccaccgc
      841 caaattgacg aaagagctcg gaaaacagct ggagcttcag ttgaccgcta cggcatgccc
      901 gtcgcgtgcc agctgccgta cgcggagcgt ggacactgtc gcgccaaaca cctgccacgc
      961 gcggcgacac gcctccatca cgtccgtcag gaccgcgaag gcggtcccat acggctccga
     1021 gcggcccttg ttgacatgac gcgcgccaca cctcgacaga gcccgttgca catcccccca
     1081 tgtgtcgcac ggggccgcct gcgcggccct gacgagggga cacgtgtcgc gtgaccacgc
     1141 cgtgggcact gtcacgcccg acacctgccg cgcgccacca agaaaccccg cgccactact
     1201 aatacgtctt cgccgccgcc gccggcgcct ggcccttgag ctcgcgcacg gcctgctgcg
     1261 ctgccatggc catgaggcgg gcgtcgttca ggatcgtgca gagctggaag ccctcgtcga
     1321 agcgcttcgt cgcggtcttc acgccatccg tgtggacgcc ggcgatgagg cccttggcgc
     1381 gggttttctt gcagatggtc gcaatggcgg cgagcacctc cttgtcggac gggtcgagcg
     1441 tcgcgggctt gccgagcgag atcgagagat cggagggacc gacgtagacg ccgtcgatgc
     1501 cgggcacgga gagaatcgcg tcgagattgg cgagcgcctt ggtcgtctcg atcatggcga
     1561 agagcaggat ctcatcgttg gcgttcaccg cgtagtcggc gccgccgtac tgcaccgcgc
     1621 gattggggcc catggagcgc tggccacgcg gggcatagcg gccgtagctc gcgaacgcct
     1681 ccgcctcctc cttggtgttg atcatcgggc agatgatgcc gtaggcgccg gcgtcgagca
     1741 tcttcatgat gatgccgggc tcgttccagg gcacgcgcgc catgggaacc gagtccgtgg
     1801 tcgagattgc ctgcagcatc ggcacggcgg aggtgaaatc gaccggacca tgctggagat
     1861 cgatcgtcac cgaatcgaag cccgagtggg cgatgagctc tgccgagtac gagctcggaa
     1921 tggcgagcca gccgttgatg atgcgaccgc ccttgccgac gatctcgcgg accttgttct
     1981 tgcgcatgcg cttcctcgct tggagccagt tcctgtcttg gctcgacaaa ctagcgccac
     2041 gcgacccgct cttgccagct catttccggc atgggtgcac tccaataagc agaaaggccg
     2101 ccttgcggcg gcctttcgag ttccactctc gcaggtctca gaccagcgtc agaagaagat
     2161 gcgggtgccg atgatgaccg tcgagaagtc ggaggcgctg gtgttcaggt gcgcgttggc
     2221 agcgttgaag ccggtcgcgt ccagggagaa gttcttgtag accaggaaca gctccatggc
     2281 ggcggcgtca acgttctgca ggatgcccag gccccacatg tcgacggtat tgttgctcac
     2341 cgtgtcatgc gtcgcgatca ggccgaggtg cgccgactgt gcgagaccgc ccttgaactc
     2401 gccgtactcg gcgaagagca ccgtcgcacc gagccccgtc cagttctgac ggataccgcc
     2461 ggcgatgtac cagttggtga tgtccggacc gacgtaggcg ccgggcgcgg gcagaccacc
     2521 ttcgagctca cgcttggcgt aggccgtcgt cacgaacagg ccgctgctga catgcaggat
     2581 agacagagcg cccttgaggt cggtcgtctt ggtatcgcag ttcgtcgtgc aaagcgcgcc
     2641 agcaacgttc agcagctggt tcggcgcatt gaactcggtg tcctcgctgt agccgatgcc
     2701 aaaggcaacg cggaagccgt gatgctcgcc agcgtagcgg agcgcgacat cccagtagtt
     2761 gtcttcagcg accgcggcct gcagcgtgaa gccatgcagc gacggcgtct cgtagaggac
     2821 gtggttacgg cgcgaagtat cgaacgactc gtggccgcgc atcgcagcag cccagttggt
     2881 ggcggtcgag gaggcgagcc cgccggggcc acggagaata aagccaccgt tgtaaaggct
     2941 ggcgtccatc gtcgccgcgc cgccgaggcc acccagatcg atgaggacca ggttgtcggt
     3001 cgcggtcgaa ccgtgaccaa ccttgatcat gccgagctga ctgtgacgca accagacagt
     3061 cgtgtcacgg agagcaacgg agccaccgag gtcgtcgccg ccattcagct ggttgaccgc
     3121 gttgacggcg ttgttggcaa tgccgaactc gtagaggaag ccggccgtga cgttcggcgt
     3181 gatcttcgcg gtaccgacga agcggaagcg cgagccgtag ttgccgccgt cggcgatgta
     3241 catgtccgac tgcgtgccgt cgtcccagaa catcacgttg tgggcaacga agccggagat
     3301 cgtcagcgac accttgcggt tgcccttgcg gacggtcgtg gcctcgagct cggcaacgcg
     3361 ctcctcgaga tcggcgcagc agttcccgcc aaggtcggcg gcgttcgcca tcgaggcgga
     3421 agccatcagg gctgccgcgg cgagggcata tcgactggtc gtcttcatta gtcccccaat
     3481 ctttccaggg tcgtccatcg ttgagttcgg gaagcattac gcgcagcccg gcgatctgtg
     3541 gcaccgtgtt cgctctgagc ggcacgcacg cgcgtcagcg cacgcggctc agaccgaacg
     3601 cggttactcg aacaacactg acgcggaact agatgagcgt gcggctctta ctcgcggcgc
     3661 cgggatgctt cccgtcgggt caccatggac gattgcctga tgtccctgag gtcttcttgg
     3721 accccactcc gcacgactcg actgggatga ttgatagaag agtgggccgg ggcttggaaa
     3781 cagaaagcgt cggctttccc gtggttcgat tgccacctgt ggctgcatcg acacaaaaga
     3841 gttggtggat agggcgcgta tgtcgcatgg cttttgtcga ggtcagcgat tcgtgttgcc
     3901 tggtccgcgt cgcgcgttgt tgcgccgccc tttttcgaag gcttgccgat tcgcaggtcg
     3961 tcactcgagg cgaatgagtg ctacttcggt aggcatgcct tcttgatacc gggcccggca
     4021 atcgctgctg tcggaggaag gctgcgagga cctnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnngc cgtaggcgag agcgatctct cctccggctt tgatctccgt
     4261 aagctcggtc ggcgaaagcg taccccgctg cagcttgagg tcgccctcat acaacctcct
     4321 gcccttcgcg tctgttcggg ccgggtagag ttgggccgat cgctcggagg tcaggaatgc
     4381 cggcaggcgc cgtattcgct tacgctcgat gagctccttc cagacgatca ggttgttgtg
     4441 ggcgacgacg ccagtcggcc gatccgccgg aatgacgcca tctcggcgca gtcggtcctc
     4501 gatgtcgtgc gcccaatcga cgatcgtctt catgacggtc gcatcgcgcg ggtgtcccga
     4561 gcccgcgggc caatgcaggt tgtcgatacg gatgtagttc gcgccgatcc gccagccgtt
     4621 ctcgatgccg gcctcgagac ggcgccgcga cgccgcggag aggatgttga tgacgcgccg
     4681 ctcgctcttg tcccagttgt agctgccggt ggtctcgagc acggcgtcgg ggccgaggct
     4741 cgcgcaatcg cggccgagat cgcagtgacc gccgaccaga tagatcgaga cgcgcgcacc
     4801 gagcgcctgc agctctctga cgtattgcgg acctgccgcc tttggcattg tccgcggatc
     4861 gatctcgacg atcatccctt ccttgaagtt cgagagcgcc atccgctcct cgatggctgg
     4921 gaagagcccc gcgaatgcaa acggcgcaaa gccgggcgct ggcggcgggc agctctgagg
     4981 cgcaccctgc gctaccgctg caccagcgaa cagccgagtt gccagagcaa ttgctgcgca
     5041 acaagaaacg actcgacttg ggcgcacgca acacctccga tagcgcagga acgcaccata
     5101 ccctgtagca tctatcatgc cgtgccgcga tcgtgttgcc cctccgcgac gcccccgagg
     5161 tcaggcgtgc agagacaagc acccctgcgt gctgctccaa ttcggtcaac gggcagagtg
     5221 cgtgcgcgac tgtggtatat ctgccgcgca tccacaccaa gacggaagta tttgttgcac
     5281 cgcaaccttt tgctgctagt gtgcattgcg gcaagtgggg tgtgcatcgc gccatcgagt
     5341 gcgcaacatc atagcgacgg ggaaagacgg acatgacagt cgtcacacgc aagagctacg
     5401 ctttcgagga tcttcagctc ggcatggaag cgagcgtggt tcgcaacgtg agcgagtccg
     5461 acatcgtcac gttcgccgag atctccggcg acaagaatcc gctccacctc gatgccgcct
     5521 tcgccgccaa gaccatgttc aagcagcgca tcgcccatgg catgctctcg gcgagctaca
     5581 tctctgccgt gttcggcatg gagatgcccg gcccaggcgc cgtctacatc tcgcagacgc
     5641 tgaacttcaa ggcgcccgtg cacatcggcg cccgcgtcgt cgccaaggta aaggtcgtcg
     5701 agctgatcgc cgccaagaag cgcgcccgct tcgagtgcgt ttgcctcgtc gatggcaagc
     5761 cgatcctcga gggcgaggct gtcctcatgg tgccctcgcg cgcctgacga cgagccggca
     5821 aacagccctt cacgatcatt acggcgcggc ctatctagtc ccgggactcc cgctgtcggg
     5881 acacctcggt tcaagatgag gccgcgcagt cgttcatgct caaggtcatc cacggatttt
     5941 caagcgtgcc cgccgaggcg cgcggcgctg ttctggccat cggcaacttc gacggcgtgc
     6001 atcgcggcca tcaggcgttg atccgcgagg ccaagcgcat cgccgccgcg acgcccgcgc
     6061 caggcgcacc gagcggcgtg atcatcttcg atcctcatcc acgtgagcac ttccatcccg
     6121 accggccaca tttccggctt acggcactcg atcgcaaact cgagcttttc gacgcctatg
     6181 gcgcaaggat tgctgttgtc ctcacattcg acgcatcact cgccggcatg accgccgagg
     6241 cgttcgtcag ccgcgtgctc gtcgaggggc tcgcagtccg ccacgtcatc gtcggctacg
     6301 acttccattt cgggcggaac cgcggcggcg atgtgcgctt tctcgagagc gcgggcctca
     6361 ggaacggctt cggcgtgacc gtggtgccgc ccgtcgccga gggcggcgag gtgttctcct
     6421 cgagcgcgat ccgcgcggag ctcgcgcaag gcgatgtcga gagtgccgcc gaggcgctcg
     6481 gccactggtg gcgcgtctcg ggcatcgtga tcggcggagc ccacctcggc accggcttcg
     6541 gcttcccgac cgccaacatc gatcttccca agtgcgccgg cctcgcacat ggcatctacg
     6601 cagtgcgcgt gatcctcgcc gatggccgcc gcttcagcgg cgcggcctac ctcggcaccc
     6661 gcccgaccgt cgacgacggc ccgccgaagc tcgagacctt cctcctcgat ttctcgggca
     6721 acctctacgg ccaagtcatc actctcgagt tcgtcgcgcg catccgtggc gacaagcgct
     6781 tcgccgactt cgaagagctg aaggcgcaga tggcgcgcga ctgcgaggag gcccgccggc
     6841 gtctcgccgc cgcgcccgaa acccccgtct gaggcgcaaa gcgctcctgc acgcatcgca
     6901 gatgacctgc agcgcaagtg ccgctacgct cgttcccgtc ggccgcggag ggagagccaa
     6961 tgcccaacat gcaaggaaag cgcgcgttcc tggagctgct gcgtcaggag ggcgtcgaca
     7021 agatcttcgg caatccaggc accaccgagc tgccgctcat ggacgcgctc gccgtcgaga
     7081 cgggcataga ctatgtccta ggcctcaacg aatccgccgt catggcgatg gcggacggct
     7141 atgcgcaggc ctccggaaag gtcgcggtct gcaacgtgca cgttgcgccc gggctcggca
     7201 acgcgatggg catgctctac gacgccaaga aggcgggctc gcccgtgctc gtcaccgctg
     7261 gccagcacga ccaggccttc accttccagg agccgatcct ctgggacgat ctcgcgacga
     7321 tggcgcggcc gctcgtcaaa tggtcggtcg aggtgacgcg cctcgtcgat cttccgcgcg
     7381 cggtgcatcg cgccatcaag actgcgctcg cgccgccgat gggaccggtg ttcctgtcgc
     7441 tgcccgtcga cgtgctccag gataccgcag agatcgacct tggccggccg acgcgcgtcg
     7501 cccgcaacct gcgcggcgac cccgaagccg tcgccgccgc ggcgaagcta ttggcccggg
     7561 cgaagtcgcc cgtgctgatc gcaggtgatg ccgttgccca atccgatgcc cacgccgaga
     7621 tggtggcgct cgccgagctg ctcggcgcgc ccgtctacgc cgaaggcatc ccgagccgcg
     7681 caacgttccc gtcgactcat ccgctcttcc agagcaacat gcagcgtcta tcgccgcaga
     7741 tccgcgcggc cctcgagccg catgacgtgc tcttctccgt cggcggcgac ctgttcaccc
     7801 agtcgctgcc gaccaaggcc gatccggtgc cgccgacgac ggccatcatc cacctcgaca
     7861 ccgatccctg ggagctcggc aagaaccacc cggaggagat cgccatcctc ggcgatccga
     7921 agtcgacgct gcccgacatc atcgcggcct tgaaggccga gctcggcgcc gaaggtctca
     7981 aacgcggcgc ggtccggctc gatgccgcaa aaaagtctgc cgcgacgcgt ctcgccgcac
     8041 tacacaagga ggccgacgcg ctcgcatccg caacgcccgt gaagccgctc gcactcatga
     8101 aggcgatcgg cgacctcgta ccgaaggacg ccgtgatcgt cgaggaggcg ctctcctccg
     8161 ccgatcgcat gcgcttcctc atcccctcgc tcgattcgaa gagctggtat gggctccgcg
     8221 gaggcggcat cggctggggc gtgccagcga cgatcggcat caagctcgcg cagccgagcc
     8281 ggcccgtcgt cggcatcatc ggcgacggca gctcgatgta tgcgatccag gcgctctgga
     8341 gcgccgcgca tcacaggatc ggctgcgccg tgttcgtgat cctcaacaac ggcagctatc
     8401 gcatcctgaa gcagcgcatg aacgccatgc gagagtacgc ggcgcaggcc gaccactatc
     8461 cggccatgga cctcgtcgac ccggccatcg atttcgtcgg actcgccgca tcgctcggcg
     8521 tgaagggcgc ccgcgccggc tccgtcgcag agttccgcga catcttcaag cgcgccgtcg
     8581 cgggagatgc gcccttcctc atcgacgttg cgatggatcg cgccttcaag ccggtctgag
     8641 aaacgctcac aacaagcggc cccagcctcg tgggtggagg ccggggccag tccagagtat
     8701 gggccccgaa agccctggac gatcaggaaa cccgccgctc agttgatcac gcaggcgttg
     8761 ctcagcagcg cgtcgagatc gaggtgcacg ccctcgagca ggacgacgtc gacgaaaccg
     8821 gcgtccccca tgtcggccga gacgatcgtt ccggccgccg tatcggtgac gcgcagcatc
     8881 gacgaggccg catccctgcc gcagaacagc gacgagaagt cgagccgatc gcccaccatg
     8941 aagtcgcgga tgcgatcgag gcccgtgccg atgtcctcgc gctcccacac gaacgtgtcg
     9001 ttgccggcag agaagccgtt gcgctgctgn nnnnnnnnnn nnnnnnnnnn nnnggagccg
     9061 cgcccgcccg cgatccgatc gtcgccgccg tcgccgtaga tcgtgtcgtt gccgtcgccg
     9121 ccgaacacga tgtcgttgag gcctccggca tagatgccgt cgttgcccgt gccgccgtag
     9181 atgagatcga agccgagacc gccgttgagg aagtcgtcac cgtcgccacc gtcaagcgtg
     9241 tcgtcgccgt tctggccgtc taggtgatcg ttgccggcac cacccgtcat ccggtcgttg
     9301 ccctcgccgc cgaatagacg atcgcggccg gcgccgccgt cgaggtgatc gttgccgccg
     9361 tcgccgtaga gctggtcgtc gccgtcgccg ccctcgagac ggtcgttgcc gttgccgccg
     9421 cgcagcgtgt cgttgccggc ctcgccgtac atgaagtcgt tgccctcgcc gccgtccagc
     9481 tgatcattgc cgctgccgcc gtagagccag tcgacgccgt tgccgccgga caggatgtcg
     9541 tcgccgtcgc cgccgaggag ttcgtcgctg ccttcgccgc cggaaagctg gtcgttgccg
     9601 gcgccaccgt cgaggagatc gttgcccgca tcccctctga ggatgtcgtt gccgttgccg
     9661 ccgtagacct tgtcgttgcc ggtgctgccg acgacctggt cgtcgccgtc gcccgcgtcg
     9721 atcgtgtcgt tgcccgcggc gccatcgatg atgtcgctgc cgccaagacc attgatccgg
     9781 tcggaagacc gcgtcccgac gatgacgttg ttgccactgg tgccagaaat cgtcgccatg
     9841 tgctgctccc tggaacgccg gcctgccgat ctcgcgccgg cgcgccgggt tcttcccgac
     9901 tcggatacat ctactcacga tcgacagtag atgagagagc ttgcggcacg gttcgtgcgc
     9961 ggcgcacaaa ttgcgccgaa gtcgagctga aattgccgag aattagatcg atcgcggctg
    10021 cagctcactc gccgcggtcg tgaaagaaat cgtaaacctt ctgcgccatc tcggcggaaa
    10081 tcccggaaac ggcctggagg tcggcgacgc ccgcccgcga cacggccttc gccgagccga
    10141 agtggccgag cagcgcgcgt ttgcgcgtcg ggccgatgcc ggcgatctcg tcgagcggat
    10201 tggcgctcag cgccttggcg cgcctggcgc ggtgcgtgcc gatcgcaaag cgatgcgcct
    10261 cgtcgcgcag ccgctgcacg aaatagagga cgggatcgcg cggctcgagc atcagcgagc
    10321 gctcgcgtcc ggggatatgg aagagctcgc gccccgcgtc gcgatccggc cccttcgcga
    10381 cgccgatcag tgtaaggtcg gcgagcccga gttctgctgc aacctcacgg gcaacggcta
    10441 gctgacccgg tccgccatcg atcagcacga ggtcgggccg ctcggggaga tcctccatga
    10501 gggctgcatc cgcgtcgaga tcggcctcgg tggactgcga tgcctcgtcg gcgccgtcca
    10561 ctccctcgcc ctgcgaattg ttaccactgg ctggaggcgc gacctcgtcc cggcgtgccg
    10621 gcgcctccgc cgtcgcatgc gcatccccat cggcggcacg ctccgttccg ccatctgctg
    10681 tcgccaactc cgcgagccgc ttgaaacgcc gcgtcagcac ctcgcgcatc atcgcatagt
    10741 cgtcgccggg ggtcgtcgca tcagacttga tgttgaactt gcggtactgg cccttcacga
    10801 agccgtcgac accggcaacg atcatcgcgc cgacagcatt ggttcccatg atgtggctgt
    10861 tgtcgaaaac ctcgatgcgg cgcggcacgc gcgggaggct gaagcgctcc gtcagcgcct
    10921 cgagcagacg gcgttgcgag gaggtctcgg cgagcttgcg tccgagcgcc tcgcgcgcat
    10981 tcgtcagcgc gtgctcgacg atctcggact tggtgccgcg ctcgggcacg cggatctcga
    11041 cgcgccgctc tgccttgctg ccgagcgcct cggccatcag ctcgcggctc tcgaagtcct
    11101 cgctgacgag aatgagacgc ggcaccggct tgtcgtcata aaactgagca atgaagctct
    11161 ccagcacctc gcccgccgca aacgagcggt ccgcgcgcgg aaaatacgcg cggttgcccc
    11221 agttctgccc ggtgcggaag aagaacacct ggacgcacgt ctgaccgccc tcctggtggg
    11281 ccgcgaacac atccgcctcc tcgacattgc caggattgat ggcctggtcg ccggtcacgt
    11341 gcgcgagcgc ctgcaggcga ttgcgcagcg ccgccgcccg ctcgaactcg agcctgtcgg
    11401 cggcctccat catcatcgcc tgcagctgcg cgcgcacatc ctggctgccg cccttcagga
    11461 agcggatagc ctcggcgacc agccggtcgt agtcctcgcg tgagatctcg cccgtgcagg
    11521 gcgcagaaca gcgcttgatc tggtgcagga ggcacggccg cgtgcggctt tcgtagacac
    11581 tgtcgttgca ggtcctgagg aggaatgcac gctgcagggt attgatcgcg cgcgcaaccg
    11641 cgcccgccga ggcgaagggg ccgaaatagt cgcccttgcg gctccgggcg ccgcgatgct
    11701 tcaggatctg cggcgccgcg tgatcgcgcg cgatcaggat gaagggaaac gacttatcgt
    11761 cgcgcagcag cacgttgaag cgcggccgga agcgcttgat gaggttcgcc tcgaggagca
    11821 gcgcctccga ctcggtccgc gtcgtcacga actccatcga agccgtcgcc gcgatcatgc
    11881 gggcgatgcg gttcgagtgg ccgccgaggc gcgcgtaatt cgagacgcgc gccttcagcg
    11941 agcgcgcctt gccgacgtag atgacctcgg cctcggcatt gagcatacga tagacgcccg
    12001 gcgagccggg gagcgtcttc agataggcgg caatgacctc gggacccgtc gccgaagccg
    12061 ttgccatcga cgacgcccca tcggccggca cgccgccgct ctcagcggga tcgctggcgc
    12121 tcgtcatcgc atgtgctcgt tcgatgggcg ccgactcaac tcttgccgag ccagcgcttc
    12181 cagaagccct tcttcttctc gcctccggca gcaccggcat cggcggcatc ctcgccggag
    12241 acgaccttca cgaagttgcc gaagaactcg tcggcgagct tctggctcgt cgccgtgatc
    12301 agccggccgc cgagcgtcgc gagcttgccc ccgacatcgg ccctggcggc gtaactgaga
    12361 accgtcgcct cggggccatc ctcggcgaga cgcacgtcgg cgtgcccctt cgcgaacccg
    12421 gcagccccgc cctgcccctg ccccgcgatc gtatagctct cgggcgcgct gatgttgctc
    12481 agtgtgactg cgccgttgaa ggccgccgag accggcccga tcttcagccg cacacgcgcc
    12541 gccatctcag tcggcgactt ctgctcgagc gtctcgcagc ccgggatgca ctggcgcagc
    12601 acgtccgggt cgttgagcgc ggcccagacg acatcgcgcg gggcggcgat ccgatattcg
    12661 ccgttgagat ccatgccact gctccctgat cacaggccga cgagcctcag cccttctgac
    12721 cgagcaaagc cccaacgtca attcggctgg tgtgtcctca cgctcagggc cgcttgcgca
    12781 tgaaggcctg acgccggcac tcggcgacca agtcgcccgt ctggttgaag ccgcgatgca
    12841 cgaactcgac aataccagcg tcgggccgcg acttgctctc gcggcgcgcg accacctccg
    12901 tctcgcagcg cagcgtgtcg ccatggaaca ccggcttcgg aaacttcacg tcgctcatgc
    12961 cgaggttggc aacggtcgtc ccgagcgtcg tgtcgttcac cgagatgccg atgaccacgc
    13021 cgagcgtgaa cagcgagttg acgagagggc gcttccactc cgtctgagcc gcgaaatgat
    13081 ggtcgatgtg cagcggctgc gggttatggg tgagacagca gaagagcgtg ttgtccattt
    13141 ccgtcactgt ccgggtgatg gcgtgcttga acacgcggcc aatctcgaac tcctcgaagt
    13201 agaggccatg tgccatgtcg atcctcctgt tgcggctcgc cggactgtcg cccgcccgcc
    13261 cgccgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg cggaggtatt cacctacgag
    13321 attgaccagc gcgccttgag ccgtgcgcga ttcgcactat cgcgatttgt acttgcacat
    13381 ctgagggaga tggattagaa caatacggga acttcgaaga ggcgcccggc acgcacgaag
    13441 tgcggttgca gtcgaagtac gacaattcgg tggagaaccc gcaagcactg cgacttcggc
    13501 cctgccgacg gcaaacgatg acgctacggt ccgcgcggaa cgatagcgtt ggctatccga
    13561 aacacttggt ctgaggagcg acagccatgg ccggttccgt caacaaggtc atcctggtcg
    13621 gcaatgtcgg caaggacccc gagatccgcc gcacgcagga cggacggcca atcgcgaacc
    13681 tacgcatcgc cacgtccgaa acctggcgcg acaagaacag cggcgagcgc cgcgagaaga
    13741 ccgagtggca ctccgtcgtc gtattcaacg agggcctctg caaggtcgtc gaacagtacg
    13801 tcaagaaggg cgcaaagctt tacatcgaag gccagctgca gacgcgcaag tggcaggaca
    13861 aggacggcca ggaccgctac tcgaccgagg tcg
//
LOCUS       DAORNH010000097        13884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3337_length_13884_cov_2.411527, whole genome shotgun sequence.
ACCESSION   DAORNH010000097 DAORNH010000000
VERSION     DAORNH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_13884_cov_2.411527"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1348
                     /locus_tag="PK264_11450"
     CDS             149..1348
                     /locus_tag="PK264_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76538.1"
                     /translation="MGRMRAGETKLNERMNSGDRARYYKVTTTDTAILSASVARTTGD
                     IELVILDSTGRELDKTTRSNRDKVWTADIGRPAGTYYVAVINSGRSSTPFDLTFNIVM
                     PAPAHSHIDLAEKLSLGSGDTTFRSSFRDETKFRYAEFEVRRGGLYKLTLETQRADRD
                     ADIDIRLYKANPGWKEIARAEKGPGESEVLVEALEPGRYLIQLYRDAGREAEVLVTIS
                     QTNDMVDPEVEGQVIATHQDWTTVMRRRDGKKQCVIYTYAKSVSPAGWRGPRPVLQLR
                     IDEDEDGVFHTFDKVRFYGTNLRWDANVTGASANFKVPVMVKDDESDLKSLEPCIQNK
                     NELCVADDGLIALTLGRELTLKGTTADGRSSTVVFSLLGYQKSVDEMNRECDNERRTG
                     WLKKRKR"
     gene            1553..2455
                     /locus_tag="PK264_11455"
     CDS             1553..2455
                     /locus_tag="PK264_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76539.1"
                     /translation="MPDRDAPARGSPKIQSFEEQRSATAGPLAELAEVDAPPAIKAIY
                     ADIKRLTGVPMVALIFRHLATRPGALEDAWRVLSPAFVSGRIQSVAWWLAEDAECGPI
                     VPALAPATRLALGIDAAGEAAVSMTLDAYNRANPVNMLAMLSLMERHRLGAAPARPLA
                     SEAAMWSAPAPITGPLPRMTPVGEIAPDLRHLINDLGFGDRSELDSVVPSLYRHFTPW
                     PALLGLLHATAGPAFRDGSLAFKVETLHDQMRAKAAEIAPTLAPLEHGDGIAAALPTL
                     RRFTSSVIPQMVLVGFALKTALAG"
     gene            2572..>3929
                     /gene="pyk"
                     /locus_tag="PK264_11460"
     CDS             2572..>3929
                     /gene="pyk"
                     /locus_tag="PK264_11460"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRD76540.1"
                     /translation="MRRDRKAKIIATLGPASSEPAAIRRLFEAGADVFRLNFSHGTQA
                     DHKRRFDIIRALEKEVGRPIGILQDLQGPKIRIGTLKNGPVKLERDETVTFTLGSEPG
                     DAKRVSLPHPEVFAAVLPKHRLMIDDGKLQLEVERCLDGAFEARVVVGGMLSDRKGVN
                     LPSSELAISPLTAKDRADLTFGLELGVDWVALSFVQRPSDVIEAQEIIKGRAGIMAKI
                     EKPAALERITDILALVDAVMVARGDLGVEIPPEDVPGRQKELVRLCRNAGKPVVIATQ
                     MLESMINSPSPTRAEASDVATAIYDSADAVMLSAESASGSYPFDAVAMMERIISHTET
                     HRLYRSIIEALQPSPEQSPAHAVAEAAASIADQIGAAAIVSFTASGTTATRVARKRPR
                     VPILSLSPRLETSRRLALLWGVHCVTSDEIATYDEMVARARAHAIDEGFATVADLYDG
                     CK"
     assembly_gap    3930..4124
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4125..4433)
                     /locus_tag="PK264_11465"
     CDS             complement(<4125..4433)
                     /locus_tag="PK264_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76541.1"
                     /translation="MSEPTSATTADASDDLPRTLRREREARYREVMDRVSRETSPAGP
                     VPSAGPSPTFGAPDGGNAHAHLAAMDLDDGRGVTVTALQVPFMRLVLFFLKAALAAIP
                     A"
     gene            4741..4825
                     /locus_tag="PK264_11470"
     tRNA            4741..4825
                     /locus_tag="PK264_11470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4775..4777,aa:Leu,seq:tag)
     gene            4912..5454
                     /locus_tag="PK264_11475"
     CDS             4912..5454
                     /locus_tag="PK264_11475"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRD76542.1"
                     /translation="MQVTEASVDGLRRNLKIVVGAQELTDRLSKRIDELKDQIQLKGF
                     RRGKVPPGHIRKVYGRSIMAEMIQQTVEESSRKAISDRGERPAQTPEIDLTEDKAEIE
                     KVIDGKSDLAYLLKYEALPKIDLGDFGALKLERLVADVPADAVEKALGELQERNLRYE
                     AEEGRTRSRSPRSLTTSPRA"
     gene            5451..6482
                     /gene="tig"
                     /locus_tag="PK264_11480"
     CDS             5451..6482
                     /gene="tig"
                     /locus_tag="PK264_11480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRD76543.1"
                     /translation="MTTFEDQAGRKAEQGDRVTIDFVGRIDGVEFEGGKAEGVPLVLG
                     SGGFIPGFEAGLEGAKAGEERKVSVTFPADYPKAELAGKQAEFEVKVHAVGKPVKPAI
                     DDDFAKGFGAESLADLRKRVEEQIGREYGDVARQKLKRALFDEFDKTQSFALPQTLVD
                     SEFDGIWKEVTGNLERTKRTLADEGKTEESARAEYRKIAERRVRLGLLVGEIGDRHKI
                     EVTEEELKAALVGQARRFPGRTRELMEFYQKNPGALAHLRAPIFEDKVVDFVLGKATV
                     SDRKVSSEELLQPLPDDEAAVTAQDDSHAHHGHDHHGHDHGHDHAHDHAHDHKHDHGH
                     GHDHGRKPA"
     gene            6591..7223
                     /locus_tag="PK264_11485"
     CDS             6591..7223
                     /locus_tag="PK264_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRD76544.1"
                     /translation="MAHPVETALDLVPYVIEQSARGERGMDIFSRLLRERIVFVTGPV
                     HDGMAAVVTAQLLLLESENPKKEISMYINSPGGVVTSGMAIYDTMQFIRCPVATLCIG
                     QAASMGSLLLCAGNPGMRFALPNARIMVHQPSGGFSGQASDIERHAEDIIKMKRRLNE
                     VYVKHTNQKYETIERTLDRDYFMTSEQAQEFGIIDRVLTKRDDIDPAKKV"
     assembly_gap    7521..7719
                     /estimated_length=199
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7797..9047
                     /gene="clpX"
                     /locus_tag="PK264_11490"
     CDS             7797..9047
                     /gene="clpX"
                     /locus_tag="PK264_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRD76545.1"
                     /translation="MPQEKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELCMDIIR
                     EENKNTLVKSRDGVPTPQEICGVLDSYVIGQFHAKRVLSVAVHNHYKRLNHAAKNNDV
                     ELAKSNILLVGPTGCGKTLLAQTLARMLDVPFTMADATTLTEAGYVGEDVENIILKLL
                     QNADYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTVASVPP
                     QGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIISRRTIGGSVGFGAKVVSPEERRTG
                     DILREVEPEDLLKFGLIPEFIGRLPVIATLEDLDEKALMQILTEPKNALVKQYQRLFE
                     LEDVRLTLSQDALKAISRKAIERKTGARGLRSIMEGILLDTMFDLPAMKGVEEIVIGP
                     EVVEGGARPLRIYSERAEEKGTTA"
     gene            9334..>11716
                     /gene="lon"
                     /locus_tag="PK264_11495"
     CDS             9334..>11716
                     /gene="lon"
                     /locus_tag="PK264_11495"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRD76546.1"
                     /translation="MSDDKTPKDKSARTRLPVLPLRDIVVFPYMIVPLFVGREKSINA
                     LEEVMRTDKQILLVAQKNATDDDPTTEGIYQVGTLASVLQLLKLPDGTVKVLVEGSVR
                     AKIVRYVENPEYFEADIERIAETVGNTTEIEALARSTSSQFESYIKLNKKIPPEVLAA
                     VGQIDDHSKLGDTIASHLAVKIADKQEVLEMADVSARLERVFALMENEISVLQVEKKI
                     RSRVKRQMEKTQREYYLNEQMKAIQKELGDGDERDDIAEFEEKIENTKLTKEAKDKAL
                     AELKKLRQMSPMSAEATVVRNYLDWLLSIPWGVRGKIKKDLKEAQEVLDKEHYGLEKV
                     KERIVEYLAVQARTNKLKGPILCLVGPPGVGKTSLGKSIATSTGREFVRMSLGGVRDE
                     AEVRGHRRTYIGSMPGKIIQSMKKAKRTNPLFLLDEIDKMGQDFRGDPAAALLEVLDP
                     EQNHTFNDHYLEVDYDLSGVMFVTTANTLNIPPALLDRMEIIRLAGYTEDEKIEIAKR
                     HLIPKSRVDHGLEEGEWVVEDDALTDVIRRYTREAGVRSLEREISKLARKAVKEILSD
                     GKAKVVATRDNLADYLGVPKYRFGEAELEDQVGIVTGLAWTEVGGEILTIEGVMMPGK
                     GKMSVTGNLRDVMKESIQAANAYVRSRSYEFGISAAMFEKKDIHVHVPEGATPKDGPS
                     AGVAMATAIISVMTGIPVRKDVAMTGEITLRGRVLPIGGLKEKLLAALRAGIKTVLIP
                     EENVKDLADIPDNVKGALKIVSASRLDEVLKVALVRMPQAIPMEPEVVPGPNDK"
     assembly_gap    11717..11741
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12055..>13474
                     /locus_tag="PK264_11500"
     CDS             12055..>13474
                     /locus_tag="PK264_11500"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HRD76547.1"
                     /translation="MNNILMYLGGLVVLVLGALFAVPLFVDWNSYRGAFEEEASRLIG
                     REVRVGGRVSLRLLPTPTVRLEKVRIADSSGQTGEPFFRADTFTLYLAIAPLVQGTIE
                     ANDIELTRPVLHLAVDESGQGNWRAIKRSEGRAGFSAQDIALPSVKVVDGTIALHGLK
                     GEERLRFEKVTGEVGTPALEGPYRVRLTFNHGGVERELRMSTSRPETDGSVRLKANLR
                     ETGTPTSYLVDGKLKDFLGATTVDGEISAKLPLGGPARRAAGKSEADDLVDLKSTFKA
                     NASEFSLDDLAMSFESGGRPQLVSGTIKGQWREALSLDATLGSRWLDLDRLGGTEKAE
                     IGPLARSLGNRMAALFPSAARASLRVAVDQATINGDILSQIIIALDRTATAVTVRELR
                     ATMPGGSRLDLRGVAGLDGSKPARFDGEVTVRGSSISRMLAWSLGKSAPALRRDSAFG
                     LRGKLSIESGLVLVNDMMAELAG"
     assembly_gap    13475..13576
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13577..>13884
                     /locus_tag="PK264_11505"
     CDS             <13577..>13884
                     /locus_tag="PK264_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76548.1"
                     /translation="GTLGMDEVIAELAPRLAAPRSAAAAAAKPADGLAGLDIVVRLNA
                     GRVSIADRKFRNVVADISREAGRLRIGTLRLATDDGIAIEMQGDVAGLATLAAWTGLT
                     "
BASE COUNT         2500 a         4424 c         4378 g         2061 t
ORIGIN      
        1 cccggtctcg acacccaaag tgacaacgag cccgccgcca ccgccgccgc cgccaccgcg
       61 ggtcacgcca ccgccgccgc cgtccaagac cgtgacgacg ccgcccccgc cggcagcggc
      121 cggtgattcg atcgccacgg cgcgcgacat gggtcgaatg cgcgcgggcg agacgaagct
      181 taacgagcgc atgaattccg gcgatcgcgc gcgctactac aaggtgacga cgaccgacac
      241 ggcgatcctc tcggcaagcg ttgcccgcac cacgggcgac atcgagctcg tgatcctcga
      301 ttcgacaggc cgtgagctcg acaagacgac gcgctcgaac cgggacaagg tgtggacggc
      361 cgatatcggc cgccccgcag gcacgtacta cgtcgccgtg atcaactccg gccgctcgag
      421 cacgcccttc gacctcacct tcaacatcgt gatgccggcg ccggcgcaca gccatatcga
      481 cctcgcagag aagctgtcgc tcggctcagg cgatacgacg ttccggtcct cgttccgcga
      541 cgaaacgaag ttccgctatg ccgagtttga ggtgcgccgg ggcgggctct acaagctcac
      601 cctcgagacg cagcgcgccg accgcgatgc cgacatcgac atccggctct acaaggccaa
      661 cccgggctgg aaggagatcg cgcgcgccga gaagggcccg ggagaatcgg aagtgctggt
      721 cgaggcgctc gagcccggtc gctacctcat ccagctctac cgtgacgccg gccgtgaggc
      781 cgaggtgttg gtgacaatct cgcaaaccaa cgacatggtc gatcccgaag tcgaaggaca
      841 ggtgatcgcc acgcaccagg actggacgac cgtcatgcgc cgccgcgatg gcaagaagca
      901 gtgcgtgatc tacacctacg ccaagtcggt ctcgccggcc ggctggcgcg ggccgcgccc
      961 tgtcctccag ctgcgcatcg acgaggacga ggacggcgtg ttccacacct tcgacaaggt
     1021 gcgcttctac ggcacgaact tgcgctggga cgcgaacgtc acgggcgcct cggcgaactt
     1081 caaggttccc gtgatggtga aggacgacga gtccgatctc aagtcgctcg agccctgcat
     1141 ccagaacaag aacgagctct gcgtcgccga cgacggcctg atcgcactca ccctcggccg
     1201 tgagctgacg ctcaagggca ccaccgccga cggtcgctcc tcgaccgtgg tcttctcgct
     1261 gctcggctac cagaagtcgg tcgacgagat gaaccgcgaa tgtgacaacg agcgccggac
     1321 gggctggctc aagaagcgca agcgctgagc tgcagcctca cggcaactgc gatcgagggc
     1381 ggcgccgaca agggttggcg ccgctcgtct atcgagagag gccgcgatcg ggaagggccg
     1441 cgagtgtcgc gcagtcaaaa tcgattgctg ttgccggggc cggtccgacg acgcggcctt
     1501 ggtactgcgg gacggcagcc gtccaggcga tcatcgagac gaggaccagc gcatgccaga
     1561 tcgggacgcc cccgcccgcg gttcgcccaa gattcaatcg ttcgaggagc agagatcggc
     1621 cacggcaggg ccgctcgccg aactcgcaga ggtcgacgcc ccgccggcca ttaaggcgat
     1681 ctacgcggac atcaagcgcc tgacgggtgt gccgatggtg gcgctgatct tccgccatct
     1741 cgcgactcga ccgggcgcgc tcgaggacgc ctggcgcgtg ctttcgccgg ccttcgtgtc
     1801 gggacgcatc cagtcggtcg cctggtggct cgccgaggac gccgaatgcg ggccaatcgt
     1861 cccggcactt gcccctgcca cgcgactggc gctcggcatc gatgcggcgg gagaggccgc
     1921 cgtctcgatg acgctcgacg cctacaatcg cgccaatccg gtcaacatgc tcgccatgct
     1981 ctcactgatg gagcggcacc gcctcggcgc cgcgccggcg cgtcccctcg cctccgaagc
     2041 cgccatgtgg agtgcccccg cgccgatcac tggtcccctg ccgcgcatga cgccggtcgg
     2101 cgagatcgcg cccgacctcc gtcatctcat caacgatctc ggtttcggcg accgctccga
     2161 gctggacagc gttgtcccga gcctttatcg acacttcacg ccctggccgg cactcctcgg
     2221 cctcctgcat gcgacggcag gcccggcatt ccgcgatggc agtctcgcct tcaaggttga
     2281 gaccctgcac gatcagatgc gggccaaggc cgccgagatc gcgccgacgc tcgccccgct
     2341 cgagcacggc gatggcatcg ctgccgccct gccgacgctc cggcgcttca cctcgagcgt
     2401 cattccgcag atggtgctcg tcggatttgc gctgaagacg gcgctcgccg gctgaggtcg
     2461 gggcgcaaga ggatcgagac ggccgcaaat ctgttgcatg gcctcgcacg ccctgtaagt
     2521 tctccactcg cgaaacactc aagagaggcg gcccgagggc cgagggtcga catgcgccgt
     2581 gaccgcaagg ccaagatcat cgccactctc gggccggcga gctctgagcc cgccgccatc
     2641 cgccgcctgt tcgaggccgg cgccgacgtc ttccggctga acttcagcca cggcacgcag
     2701 gccgaccaca agcgccgctt cgacatcatc cgcgccctcg agaaggaggt cggccggccg
     2761 atcggcatcc ttcaggacct gcagggaccg aagatcagga tcggcacgct gaagaacggg
     2821 ccggtgaagc ttgagcgcga cgagaccgtc accttcacgc tcggctcgga gccgggtgac
     2881 gcgaagcgcg tctcgctacc ccatcccgaa gtcttcgcgg ccgtcctccc gaagcaccgg
     2941 ttgatgatcg acgacggcaa gctgcagctc gaggtggagc gctgtctcga cggcgcgttc
     3001 gaggcgcgcg tcgtcgtcgg cggcatgctg agcgacagga agggcgtgaa cctgccctcc
     3061 tccgagctcg cgatctcgcc gctgacggcg aaagaccgcg ccgatctcac cttcggactc
     3121 gagctcggtg tcgactgggt cgccctctcc ttcgtgcagc gcccctccga cgtgatcgag
     3181 gcgcaggaga tcatcaaggg ccgcgccggc atcatggcca agatcgagaa gcctgcggcc
     3241 ctcgagcgca tcaccgacat cctcgcgctc gtcgatgccg tcatggtggc acgcggcgac
     3301 ctcggcgtcg agattccgcc cgaggacgtg cccggccgcc agaaggaact cgtgcgtctc
     3361 tgccgcaacg ccggcaaacc cgtggtgatc gcgacgcaga tgctcgaatc gatgatcaac
     3421 tcgccctcgc cgacgcgcgc cgaggcctcc gacgtcgcaa ccgccatcta cgacagcgcc
     3481 gacgccgtga tgctgtccgc cgagtccgcc tcgggtagct atcccttcga tgcggtcgcc
     3541 atgatggagc gcatcatcag ccatacggag acacaccgtc tctatcgctc gatcatcgag
     3601 gcgctgcagc cctcgcctga gcagtcgccg gctcacgccg ttgccgaggc cgccgcatcg
     3661 atcgccgacc agatcggggc tgccgcgatc gtctcgttca cggcgagcgg cacgaccgcg
     3721 acgcgggttg cccgcaagcg cccacgcgtg ccgatcctct cgctctcgcc gcgcctcgag
     3781 acctcgcgca ggcttgcgct cttgtggggc gtgcactgcg tcacgtcgga cgagatcgcg
     3841 acctacgatg agatggtggc acgggcgcgc gcgcacgcca tcgacgaggg gttcgccacc
     3901 gtcgccgacc tctacgacgg ctgcaagttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgccgg gatggcggca
     4141 agcgcggcct tcaggaagaa caggacgagc cgcatgaacg gcacctgaag cgccgtgacg
     4201 gtcacaccac ggccgtcgtc gaggtccatg gcggcgaggt gcgcgtgggc gttgcctccg
     4261 tccggcgcgc cgaaggtcgg cgacggcccc gcggacggca ccggccctgc gggcgaggtc
     4321 tcccgcgaca cgcggtccat gacctcgcga tagcgggcct cccgctcgcg ccgcaaggtg
     4381 cgcggcagat cgtcagaagc gtcggcggtc gtggcggatg tcggctccga catgatcgtc
     4441 tcctcggcaa tggcactacg ccaggcatga agcggcgcgg ccgcaggacg agcggcgctg
     4501 cagctgtcgc cggatacggc gaccctggcg tgattcgggg attgtccgtc aacctcccgc
     4561 gacccgcaca ctggacaacc gcgcctcgtc caatcgacga ggcatgacgc atgaatttga
     4621 ccttattggg aagcccctca ttccggtcga gaacgggtgc caaaactcgt gccaaaactc
     4681 gtgattgccg gatacgccct cgtgctctaa aagccggact tcggtcgagg ctgacgggcc
     4741 gcgggcgtgg tggaactggt agacacgcca gatttaggtt ctggtggctc acgccgtggg
     4801 ggttcaaatc cctccgcccg caccaacggt ttccggcgcc gcttgcgacc aacggatgaa
     4861 gatgcgacgt gacatgagat caacggctta tcgggacagg tgctgagcgc catgcaggtg
     4921 acggaagcga gtgtggacgg gttgcggcgc aacctcaaga tcgtggtcgg cgcccaggag
     4981 ctgacggacc ggctctcgaa gcggatcgac gagctcaagg accagattca gctcaagggc
     5041 ttccgcaggg gcaaggtgcc tcccggccac atccgcaagg tgtatggccg ctcgatcatg
     5101 gccgagatga tccagcagac cgtcgaggaa tcgagccgca aggcgatcag cgaccgcggc
     5161 gagcggccgg cccagacgcc cgagatcgat ctcaccgagg acaaggccga gattgagaag
     5221 gtgatcgacg gcaagagcga cctcgcctac cttttgaagt acgaggcgct gccgaagatc
     5281 gacctcggcg atttcggcgc gctgaagctc gagcggctcg tcgccgatgt gcccgccgac
     5341 gcagtcgaga aggcactcgg cgagctgcag gagcgcaacc tgcgctatga ggcggaggag
     5401 gggcggacga ggagcagatc gccaaggtca ttgacgacct cgccgcgcgc atgacgacgt
     5461 tcgaggacca ggccggccgc aaggccgagc agggtgaccg cgtcaccatc gacttcgtcg
     5521 gccgcatcga tggcgtggag ttcgagggcg gcaaggccga gggcgtgcca ttggtgctcg
     5581 gctccggcgg cttcatcccg ggcttcgagg cgggtctcga gggtgccaag gccggcgagg
     5641 agcgcaaggt ctccgtcacc ttcccggccg actatccgaa ggccgaactc gccggcaagc
     5701 aggccgagtt cgaggtcaag gtgcacgccg tcggcaaacc cgtgaagcca gcgatcgacg
     5761 acgacttcgc caagggattc ggcgccgagt cactcgctga tctgcgcaag cgcgtcgagg
     5821 agcagatcgg ccgcgagtac ggcgacgtcg cgcgccagaa gctcaagcga gcgcttttcg
     5881 acgagttcga caagacgcag tccttcgcct tgccgcagac gctcgtcgac agcgagttcg
     5941 acggcatctg gaaggaagtg actggcaacc tcgagcgcac caagcgcacc cttgccgatg
     6001 aaggcaagac cgaggagagc gcccgcgccg agtaccgcaa gatcgccgag cgccgcgtgc
     6061 gtctcggtct gctcgtcgga gagatcggcg accgccacaa gatcgaggtg accgaggagg
     6121 agctgaaggc ggccctcgtt ggccaggcgc gccgcttccc gggccgcacg cgcgagctca
     6181 tggagttcta ccagaagaac ccgggggcgc tcgcgcatct gcgcgcgccg atattcgagg
     6241 ataaggtcgt cgatttcgtg ctcggcaagg ccaccgtttc ggatcgaaag gtttcgagcg
     6301 aggagctcct gcagcccctc cccgacgacg aggcagcggt cacggcacag gatgacagcc
     6361 acgctcacca cggtcatgac caccatggtc acgaccacgg ccatgatcat gcccacgatc
     6421 acgcgcatga tcacaagcac gaccacgggc acgggcacga ccacggtcgc aagcctgcct
     6481 gaaccgatcg tcttgcgtcg ttgcgctgcc aggccacgcc tgtgcgacga cgccggttcc
     6541 gattggcaca tgccgcgcaa tcaaggtttg ccgagaaaca gcagggtccc atggcacatc
     6601 ccgtcgaaac cgcacttgat ctcgtcccct acgtcatcga gcagtccgcc cgcggcgagc
     6661 gcggcatgga catcttctcg cggcttctgc gcgagcggat cgtgttcgtg accggccccg
     6721 tgcatgacgg catggccgcc gtggtgacgg cacagttgct gctcctcgaa tcggagaacc
     6781 cgaagaagga gatctcgatg tacatcaact cgccgggcgg cgtggtgaca tcgggcatgg
     6841 cgatctacga caccatgcaa ttcatccgct gccccgtcgc cacgctgtgc atcgggcagg
     6901 cggcctcgat gggctccctg ctcctatgcg cgggcaaccc cggtatgcga ttcgcgctgc
     6961 cgaatgcccg gatcatggtt caccagccat cgggcggctt ctcgggccag gcctcggaca
     7021 tcgagcggca cgctgaagac atcatcaaga tgaaacgtcg cctgaacgaa gtctacgtca
     7081 agcataccaa ccagaaatac gagaccatcg agcgcacgct cgatcgcgac tacttcatga
     7141 cgtcggagca ggcacaagaa ttcggcatca tcgaccgtgt cctcaccaag cgcgacgata
     7201 tcgaccccgc caagaaggtg tgatcgagcc cgcaaggact caactagccg cgatcttgat
     7261 gccgccgaaa ttcggttccg gctgcgtgac ctgtgacgtc cggtcggggc ctggtcacgg
     7321 ctggtagtgg cgacctcgtg cccgatgcgg tcggccggga ccaggaaacc cacgtgtggc
     7381 gctggcggtc ggacctggca tcgcgctccg gtgtggccgg tcgggcgaca ggtggggcga
     7441 ggcggtcaca atgctgttgc aaacgtctcg cgccatcccc agttagttgg tgagttgtta
     7501 gtggaaactt gatttccccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcgcctgact tctcggatct
     7741 gacgtgccga ccgacaaatc ccgacgcaca atccctgggt gttgagagac cgcctgatgc
     7801 cccaagaaaa gaacaccctc tattgctcgt tctgcggcaa gagccagcat gaggtgcgca
     7861 agctcatcgc cggaccaacc gtgttcatct gcgatgaatg cgtcgagctc tgcatggaca
     7921 tcatccgcga ggagaacaag aacacactcg tcaagtcgcg ggacggcgtg ccgaccccgc
     7981 aggagatttg cggcgttctc gacagctacg tgatcggaca gttccatgcc aagcgcgtac
     8041 tctcagtcgc cgtccacaat cactacaagc gcctgaacca cgcagccaag aacaacgacg
     8101 tcgagctggc gaagtcgaac atcctgctcg tcggcccgac cggttgcggc aagacgctgc
     8161 tcgcccagac gcttgcgcgc atgctggacg tgcccttcac gatggccgac gccacgacac
     8221 ttaccgaggc gggctacgtc ggcgaggacg tcgagaacat catcctgaag ctcctgcaga
     8281 atgccgacta caatgtcgag cgggcgcagc gcggcatcgt ctacatcgac gagatcgaca
     8341 agatcagccg aaagtccgat aacccctcca tcacgcgtga cgtgtccggc gaaggcgtgc
     8401 agcaggccct cctgaaaatc atggaaggca ccgtggcgag cgtgccgccg cagggcggac
     8461 gcaagcatcc gcagcaagag tttctgcagg tcgacacgac caacatcctg ttcatctgcg
     8521 gcggcgcctt cgccggcctc gagaagatca tctcgcggcg cacgatcggc ggctcggtgg
     8581 gctttggtgc caaggtggtg tcacccgagg agcgccggac cggcgatatc ctgcgcgagg
     8641 tggaacccga ggacctgctg aagttcgggc tcatccccga gttcatcggc cgcctccccg
     8701 tgatcgccac gctcgaggat ctcgacgaga aggcgctgat gcagatcctg accgagccga
     8761 agaacgcgct cgtcaagcag taccagcgtc tgttcgagct cgaggacgtg cgcctcacac
     8821 tttcgcagga tgcgctcaag gcgatctcgc gcaaggccat cgagcgcaag accggcgcgc
     8881 gcgggctgcg ctcgattatg gaaggcatcc tgctcgacac gatgttcgac ctccccgcca
     8941 tgaagggcgt cgaggagatc gtcatcggac cggaggtcgt cgaaggcggc gcgcggccac
     9001 ttcgcatcta ctccgagcgc gccgaggaga agggcacgac ggcctgagcc gcgggtcgtc
     9061 cgcggcgggt ccttgcacgg attgccatcg atcgccatct gacgcgatgc gtaacgcgag
     9121 gtggctgggc tcgatgaagc atgaactgat gagcatgagc cttatgtgag tcggtttgag
     9181 gcgcgggtgg tgcggggcac gacagcctcg catcaggccg cccgactgtg atcatcgacc
     9241 cggtgcatga gcatcgcgga tacctcggaa agcatgggcg ccgtcatggc ccgcgatcgg
     9301 gcatgacggg ttgccgcagt caggaaggac cgcatgagcg acgacaagac accgaaggac
     9361 aagtccgcca ggacacggct ccccgtgctg cccctgcgcg acatcgtcgt cttcccctac
     9421 atgatcgtgc cgctgttcgt cggtcgggag aagtcgatca acgcgctcga ggaggtaatg
     9481 cggaccgaca agcagatcct gctcgtagcc cagaagaacg cgaccgacga cgatccgacg
     9541 accgagggga tctatcaggt cggcacgctc gcctccgtcc tccagctcct gaagctgccc
     9601 gacggcacgg tgaaagtgct ggttgagggc tcggtgcgcg cgaagatcgt ccgctacgtc
     9661 gagaatccag agtacttcga ggccgacatc gagcggatcg ccgagactgt cggcaatacg
     9721 accgagatcg aagctctggc gcgctccacg agttcgcagt tcgagagcta catcaagctc
     9781 aacaagaaga tcccgcccga ggtgctcgcc gccgtcggcc agatcgacga ccactccaag
     9841 ctcggcgaca cgatcgcgag ccacctggcc gtgaagatcg ccgacaagca ggaagtgctc
     9901 gagatggcgg acgtctcggc ccgcctcgag cgcgtgttcg cgctcatgga gaacgagatc
     9961 tccgtcctgc aggtcgagaa gaagatccgc tcgcgcgtga agcggcagat ggagaagacc
    10021 cagcgcgagt actatctcaa cgagcagatg aaggcgatcc agaaggagct cggcgacggc
    10081 gacgagcgcg acgacattgc cgagttcgag gagaagatcg agaatacgaa actcaccaag
    10141 gaagccaagg acaaggcgct cgccgagctg aagaagctcc ggcagatgag cccgatgtcg
    10201 gccgaggcga ccgttgtccg caactacctc gactggctct tgtcgatccc ctggggtgtc
    10261 cgcggcaaga tcaagaagga cctgaaggag gcgcaggagg tgctcgacaa ggagcactac
    10321 ggcctcgaga aggtgaaaga gcgcatcgtc gagtatctcg cggtgcaggc gcgcaccaac
    10381 aaactgaagg gcccgatcct ttgtctcgtc ggcccgcccg gcgtcggcaa gacgtcgctc
    10441 ggcaagtcga tcgccacctc gacgggccgc gagttcgtgc gcatgtcgct cggcggcgtg
    10501 cgtgacgagg cggaggtgcg cggccaccgg cgcacctaca tcggctccat gccgggaaag
    10561 atcatccaat cgatgaagaa ggcgaagcgg acgaacccgc tcttcctcct cgatgagatc
    10621 gacaagatgg gccaggactt ccgcggagat ccggcagcgg ccctcctcga ggtgctggac
    10681 cccgagcaga accatacctt caacgaccat tacctcgagg tcgactacga tctctcgggc
    10741 gtgatgttcg tgacgacggc gaacacgctc aacatcccgc cggccctcct cgaccgcatg
    10801 gagatcatcc gtctcgccgg ctacaccgag gacgagaaga tcgagatcgc caagcgccac
    10861 ctgatcccga agtcgcgcgt cgaccacggg ctcgaggaag gcgagtgggt ggtcgaggac
    10921 gacgctctga cggacgttat ccgtcgctac acgcgcgagg cgggcgtgcg ctcgctcgag
    10981 cgtgagatct ccaagctcgc ccgcaaggcg gtgaaggaga ttctctcgga cggcaaggca
    11041 aaggtcgtcg ccacgcgcga caacctcgcc gattacctcg gcgtgccgaa gtatcgcttt
    11101 ggcgaggccg agctcgagga ccaggtcggt atcgtcaccg ggctcgcctg gaccgaggtc
    11161 ggcggcgaga tccttacgat cgaaggcgtc atgatgccgg gcaagggcaa gatgtccgtc
    11221 acgggcaacc tgcgcgacgt catgaaggaa tcgatccagg cggccaatgc ctacgtgcgc
    11281 tcgcgctcct acgagttcgg aatctccgcc gccatgttcg agaagaagga catccacgta
    11341 catgtgcccg agggcgcgac gccgaaggat ggcccctcgg caggcgtggc aatggcgacc
    11401 gcgatcatct ccgtgatgac cggcattccg gtccgcaagg atgtcgccat gaccggcgag
    11461 atcacgctgc gcgggcgcgt cctgccgatt ggcggcctga aggaaaagct gctcgcggcg
    11521 ctccgggccg gcatcaagac ggtgctgatc cccgaggaga acgtgaagga tctggccgac
    11581 atcccggaca acgtgaaggg cgcgctgaag atcgtttcgg catcgcgtct cgatgaggtc
    11641 ctcaaggtcg cactggtgcg catgccgcag gccattccga tggagcccga ggtggtgcca
    11701 ggccccaatg acaaggnnnn nnnnnnnnnn nnnnnnnnnn nccagcggcc gtgaggccgc
    11761 ccgtcaatgc aatccggaag agcggcgccg ccgagaggct ggcgccgttc attttgttgc
    11821 ccgggtgacg gtttgcccga ttatcgccgt tttgaagaat gagatcagat gaaggcaacg
    11881 acggtcggtc gtaccgggag attgaggttc tcctgctgct cacggcatgc taaccggagc
    11941 ctatgtcgat agccggcgga gagcgttgcg atctggttgg cgtttgctgg tgaagctcga
    12001 cgggtttgtc ccgttcccgc aaaggggaag tgagcgggcg aggatcggcg cacgatgaac
    12061 aacatcctca tgtacctcgg cggtctggtc gtgctggtgc tcggcgcgct gttcgccgtg
    12121 ccgctgtttg tcgactggaa cagttatcgc ggcgcgttcg aggaagaggc ctcgcgcctc
    12181 attggtcgcg aagtgcgggt cggcgggcgt gtcagcctgc gcctgctgcc gacgccgacc
    12241 gttcggctag agaaggtgcg catcgccgac agcagcggcc agacgggcga gccgttcttc
    12301 cgtgccgata ccttcacgct ctatcttgcg atcgccccac tcgtgcaggg cacgatcgaa
    12361 gcgaacgaca tcgagctcac tcggcccgtt ctccacctcg ccgtcgacga gagcgggcag
    12421 ggcaactggc gcgccatcaa gcggagcgaa ggccgcgcgg gcttctcggc gcaggacatt
    12481 gcgctgccct ctgtgaaggt ggtggatggc acgatcgcgc tgcatggcct gaagggcgag
    12541 gagcggctca ggttcgagaa ggtcaccggc gaggtcggca cgccggcgct cgaaggaccc
    12601 tatcgtgtcc gactgacctt caatcatggc ggcgtcgagc gcgaactgcg aatgtcgaca
    12661 tcgcgccccg agaccgacgg gtccgtgcgg ctcaaggcaa acctgcgcga gaccgggacg
    12721 ccgacgagct atctcgtcga cggcaagctc aaggacttcc tcggcgcaac gaccgtcgac
    12781 ggcgagattt ccgccaaatt gccgctcggc gggccggcgc ggcgcgccgc cggtaaatcc
    12841 gaggccgatg acctcgtcga tctcaagtcg acattcaagg caaacgcttc cgagttctcg
    12901 ctcgacgacc tcgccatgtc gttcgaaagc ggcggccggc cgcagcttgt ctcggggacc
    12961 atcaagggac aatggcgcga ggcactgagc ctcgatgcga cgctgggctc gcgctggctc
    13021 gatctcgacc ggctcggtgg cacagagaaa gccgagatcg gaccgctggc gcgctccctc
    13081 ggcaaccgga tggccgcatt gttcccgagc gcggcgcggg cctcactccg cgtcgccgtg
    13141 gaccaggcca cgatcaacgg cgacatcctg agccagatca tcatcgcgct cgacaggacg
    13201 gcaaccgccg tgacggtccg cgagctgagg gccaccatgc ccggcggcag ccggctcgac
    13261 ctcaggggcg tcgcgggcct cgacggcagc aagccggccc gcttcgacgg cgaggtgacg
    13321 gtgcgcggct cgagcatctc gcgcatgctg gcctggtcgc tcggcaagtc ggcgcccgcg
    13381 ctccgccgcg acagcgcctt cgggctccgc ggcaagctct cgatcgagag cggcctcgtg
    13441 ctcgtcaacg acatgatggc cgagctcgca ggcannnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnggca cgctcgggat ggacgaagtc atcgccgagt tggcgccgcg
    13621 cctcgctgcc cctcggtcgg cggccgccgc cgcggcgaag cccgctgacg gccttgccgg
    13681 cctcgatatc gtcgtccgcc tcaatgcggg ccgcgtcagc atcgccgaca ggaagttccg
    13741 caatgtggtc gccgacatct cgcgggaggc gggccgtctc aggatcggca cgctccgact
    13801 cgcgaccgac gacggcatcg ccatcgaaat gcagggcgac gtggccgggc tggcgacgct
    13861 cgccgcctgg accgggctga ccta
//
LOCUS       DAORNH010000098        13852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3344_length_13852_cov_3.016235, whole genome shotgun sequence.
ACCESSION   DAORNH010000098 DAORNH010000000
VERSION     DAORNH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3344_length_13852_cov_3.016235"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PK264_11510"
     CDS             complement(<1..686)
                     /locus_tag="PK264_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD76549.1"
                     /translation="MPKSSLDLFAVVLLTVLCILLGIGQVAMKVGNEGISPFLQAALR
                     SWGALVLLGGWCLVRGIPVLLTGRQWLPGVMAALCFTIEFALLYPGLERTTAARGVTF
                     LYTSPLFVAVGAHFFIPGDRLSVVKVLGLLVALGGIAVVLSDRSAGAGSLTGDFLCLG
                     GAVAWGSLTVIARGSALATARAETIIVTYLWISGLLLTVLSFVIGERGIFNPTLVVVG
                     SLAYTIVFVG"
     gene            complement(689..1438)
                     /locus_tag="PK264_11515"
     CDS             complement(689..1438)
                     /locus_tag="PK264_11515"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HRD76550.1"
                     /translation="MMRQSQESQDLSRRVVLAGIAGAGLLAERTTAAAEPRLTIAVVG
                     DSLADGMWGGLYRMLQRDKRVLIVRGAKNSVGFAASDLTDMLDRAVAQGGKPDAFVMM
                     IGANDRRSFFIDGRPKALFKAPRWIELYTERVGRFMDHAGRHKVPVVWLLLPIMRASD
                     ATADAELINDIIVKAAASRPHVRLMPTRSITADDRGVYQAYFKDLKGQVRLMRAGDGV
                     HFTDPAYELMADRVLALLRAASPRFQIIGGA"
     gene            1633..2043
                     /locus_tag="PK264_11520"
     CDS             1633..2043
                     /locus_tag="PK264_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76551.1"
                     /translation="MTRTLIAASMFALLALFAPPFLGGEVAGLLSERAEAQGVPYCAG
                     PTRRCADGSVPFCDRGACRLAHSPTVVIGCLRYSCPPPKIVGPITTPIGSTCAARPLC
                     PTGTIVACRQFGRCSANVFTRPTQGCLRYECRRL"
     gene            2297..3166
                     /locus_tag="PK264_11525"
     CDS             2297..3166
                     /locus_tag="PK264_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76552.1"
                     /translation="MLRVVGRLACALALSTPVMTGQAALALDCGPLKHIEIELPSGEP
                     SMIERLSDRLVKAQCAWSLKARASAPGRLAIAYGTGAEIDTLIESVLEDFLPGYSNRG
                     YVTYPIGNSIRLFRLPEFGTTSTASTHLPQPAGGPVLLFSVGLRTNGQRIGIPKLNHL
                     AATALARKGLRIWRYLDPNPDVSPATRVGADALQFVRSIPERPRLVVLTSAARAPATS
                     PNGDPEALDLSAHLLDEVSGEWSQIWKASERISPVSECTSRIHECEAKFLMTTIWPKL
                     EGAWADEIAGKLK"
     gene            3309..>3751
                     /locus_tag="PK264_11530"
     CDS             3309..>3751
                     /locus_tag="PK264_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD76553.1"
                     /translation="MSEPALKVATAGGILRLTLNRPEKLNSMTKALGRDLAAALDAAA
                     SDDSCRVIVITGAGRAFCAGQDLSDGVYVKGGPQPDLGDTVDAYNPIILKLRQIPKPV
                     IAAVNGIAAGAGASIALACDIVLAARSASFLQAFAKIGIIPDCGST"
     assembly_gap    3752..3803
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3804..4095
                     /locus_tag="PK264_11535"
     CDS             <3804..4095
                     /locus_tag="PK264_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD76554.1"
                     /translation="PLPAEKAEAWGLIWRAIDDDKLAAEVDALAARLAIAPTYGLGLT
                     KQALDAAADNSLAEQLALERRLQSEAGRTPDYAEGVGAFLEKRKPTFTGRKA"
     gene            4092..4520
                     /gene="paaI"
                     /locus_tag="PK264_11540"
     CDS             4092..4520
                     /gene="paaI"
                     /locus_tag="PK264_11540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HRD76555.1"
                     /translation="MSRSAEETARVSADAMWAGDRATQTLGMRLESVGPGTARMSLLV
                     TEAMVNGHGLCHGGYIFLLADSAFAYACNSYNVRNVAQNCQITFIAPGKLGMRLTAEA
                     RERHRAERSGIYDATVRNEAGEVIAEFRGWSRSIGGPLVA"
     gene            complement(4528..4995)
                     /locus_tag="PK264_11545"
     CDS             complement(4528..4995)
                     /locus_tag="PK264_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76556.1"
                     /translation="MLPTDAYRSKLHDTIAALRYWVPTLRHAADVEDVETETYWRIAV
                     RPRVRAACPFELILHSDQTYDLLIAGEAFERQPVESLASFQPLLEAISSGRAVTRIWR
                     APTTGSVKAVETIVTLADGTIWQRLRTLVGGRGSSAGDWIATDRRYVPYAREA"
     gene            complement(5177..5821)
                     /locus_tag="PK264_11550"
     CDS             complement(5177..5821)
                     /locus_tag="PK264_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRD76557.1"
                     /translation="MYVPPANAMTEPAALAAHLRARPFATLVSSGGQGLVATSLPTVY
                     KGDGSGGVIECHFARANVHWKELAGAGEVLLIYPGIDAYVTPNWYPSKAENHKAVPTW
                     NYSVVHAYGRAELIEDKAQLHAHVTDLTNQHEQGAAAPWAVSDAPESYIDVMLRGIVG
                     VRITVTRLIGKAKLSQNRDQRDMAGVAKGLAERGGGDDRIMANEIADLAALKRG"
     gene            complement(5895..>7863)
                     /gene="acnA"
                     /locus_tag="PK264_11555"
     CDS             complement(5895..>7863)
                     /gene="acnA"
                     /locus_tag="PK264_11555"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRD76558.1"
                     /translation="QPVAMVIPEVIGFRLKGKLREGITATDLVLTCTQMLRKRGVVEK
                     FVEYFGDGLDNLSLEDRATIANMAPEYGATCGFFPVDGDTVKFLKATGRSAERVALVE
                     AYAKAQGMWREAGAEPIYTDILELDMATVEPSMAGPKRPQDRVTLKDAKAGFAKSVGE
                     IVKGATVGAKKAKVDGMNAEIGHGDVVIAAITSCTNTSNPSVLIAAGLVARNARAKGL
                     KVKPWVKTSFAPGSQVVTDYMTKAGLQAELDAMGFNLTGYGCTVCIGNTGPLPEPVSK
                     AIADGNLVAAAVLSGNRNFEGRVHAEVKANYLASPPLVVAYALAGTMNLDLATEPLGT
                     GSDGKPVFLKDIWPSSNEIAKLIRETVTPDTFAKRYADVFKGDAKWAGMGKARASLTY
                     GWNSVSTYVANPPYFDDIKMEPAPVGDVSKARILGMFGDSITTDHISPAGAIKKDGPA
                     GTYLIERQVRPADFNSFGARRGNHEVMMRGTFANGRIKNMILGGKEGGNTRHYPSGKE
                     MSIYDAAMQYKAEGVPLVIFAGREYGTGSSRDWAAKGTRLLGVQAVVAQSFERIHRSN
                     LVGMGVLPLIFEEGTSWAGLGLKGDEVVSIAGLTTLKPRQKLEVVIVSGDGKDTLKVP
                     VLCRIDTEDEMSYWRHGGILPYVLRNLARAA"
     assembly_gap    7864..7890
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7891..8594)
                     /locus_tag="PK264_11560"
     CDS             complement(<7891..8594)
                     /locus_tag="PK264_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HRD76559.1"
                     /translation="MSLTPTSIDTFKCRKTLKVGAKSYEYFSLPDAEKNGLPGISKLP
                     YSLKVLLENLLRHEDGRSVKAGDIKAMVTWLSAKTSTQEIAYRPARVLLQDFTGVPCV
                     VDLAAMRDAMKTMGGDPSKINPLVPVDLVIDHSVMVDHFGTADAFKKNVELEYERNKE
                     RYEFLRWGSSAFTNFQVVPPGTGICHQVNLEYLAQTIWTKQSGSTTQAFPDTCIGTDS
                     HTTMVNGLGVLGWGVGG"
     gene            complement(8767..9999)
                     /locus_tag="PK264_11565"
     CDS             complement(8767..9999)
                     /locus_tag="PK264_11565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD76560.1"
                     /translation="MAGEFPTHARIVIVGAGAIGSSIAMHLAALGEREVVVLEKSGIT
                     HGSTWHAAGLVGQYRTRLDLTRLMQASVAVYDRLQQTIPIDWRPVGSLRLASSRARLD
                     EFKAALPKARERGLDFRIVTPAEARDLFPLIDLDGVEGAAHVAGDGTIDPAAVGQGLA
                     TRARELGARILEGVLVTGYEMTGGRPSAVLTDRGRITCEHVVLAPGVWARAVGRMLGL
                     DLPVAALRHQFAVTEAIPELAKGLPGLRDPDLNFYLKPEVGRFAIGGWEANTVPAFDG
                     ETPFEFGQELFPDEIDRLEPILAAAMRRLPMLARVGLKRIINGPIPFSPDGEPILGPA
                     PGMPGVWLAVGFSAGIAAAGGAGKAMAEWIISGKPEFPLPSLDPGRFRGYPTDLGALN
                     KAGVAIYGAYYALPAATS"
     gene            complement(9989..11245)
                     /locus_tag="PK264_11570"
     CDS             complement(9989..11245)
                     /locus_tag="PK264_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HRD76561.1"
                     /translation="MTVAQAYEYDAARIAALKAAYGASGPLERAFYTSPEVLRADLDR
                     IWRRHWLYVAHGCELAEAGAWKTYAVGDDRIVLVRGRDGVVRAFHNVCRHRGARVCSG
                     ERGKSALLVCPYHAWTYELDGRLRTATEREFGLASDMLGLRPVAIKDLGGLLFVALGP
                     EPVDFEAGAGEIAAGMRHQGFCDAKLAHTIRYTVKANWKLIFENNRECYHCDTAHPEY
                     VSGTYDTARFDPRRAAEVARETELAEARFTSLGLGSAVASSAMTGGWWRVSRAPLMTG
                     WQTQSLDGRPIAPLMGRMREAKAWSQGTLRATVFPNFWQHASDDHAVATRLTPIDATT
                     TEVVVSWFVHRDAIEGRDYEVSRLLPFWQRTSEQDWSICEANQEGVSSPAYTPGPYSR
                     TREPNLQHFIDWYLGALGNKGSSDGR"
     gene            11405..12382
                     /locus_tag="PK264_11575"
     CDS             11405..12382
                     /locus_tag="PK264_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRD76562.1"
                     /translation="MSSKPFKLVMLPPQTDDYRRWAAELAALAFGVHVVVSETAADAL
                     VDIVDADAAFGPLPPEVLDRAERLRWLQAPQAAPPAGFYYPALVAHPVIVTNFREIFN
                     DHIGAHIMAYVLAFARGLHYYLPDHLARRWQPRPLDTGVVHLPETTALIVGVGGIGTE
                     AARLCRAFGMTVLGIDGRRSEPSEHLAELHTPDRLDALLPRADFVIMTVPHTPATEGL
                     IDSRRLALMKRSAYLINIGRGMTVRLDDLVRALTSGTIAGAALDVFEVEPLPPAHPLW
                     TAPNVLITPHTAGYGPYLQQRRLDILVDNCRRFAEGRPLRNVVDKAAWF"
     gene            12482..13174
                     /locus_tag="PK264_11580"
     CDS             12482..13174
                     /locus_tag="PK264_11580"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRD76563.1"
                     /translation="MKARDVMSKTVVTVTPDTTIGDVARLMSEKRISGMPVVTADGKV
                     VGVISESDLLHRSELGTEKQRNWWLRVFAEPDRLAREYAKAHGNKVADVMTSNVLSVS
                     ADDDLAKVAEILERHKVKRLPVVDGGKLVGVITRGDLVRALVGAGVAKPAAISDSAAV
                     QHAIETKLREQRWLDASMIRVIATDIEVQLWGFVTSDDQRKALRVLASEHAGGRKLED
                     RLSVSRLPVSGT"
     gene            13282..>13520
                     /locus_tag="PK264_11585"
     CDS             13282..>13520
                     /locus_tag="PK264_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRD76564.1"
                     /translation="MSRSTHSTPLVALDVVVLDTETTGLDAAAARIVQVGAVRMRAGA
                     IVAGDTFDRLVNPGVPIPKATTAIHGLADEDVQDAP"
     assembly_gap    13521..13614
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13620..>13852)
                     /locus_tag="PK264_11590"
     CDS             complement(13620..>13852)
                     /locus_tag="PK264_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76565.1"
                     /translation="EVRLLAQAANALAEQRDEAREDSSRGPGVAECGVAPDDLDPERG
                     GDAIEVVLGERRERDLGEGPDIEGGGAWPGEA"
BASE COUNT         2300 a         4745 c         4399 g         2235 t
ORIGIN      
        1 aagccgacga acacgatcgt gtaggcgagc gagccgacca cgaccagcgt cgggttgaag
       61 atcccccgtt cgccgatcac gaacgaaagg accgtgagca gcagccccga aatccacaaa
      121 tacgtgacga tgatcgtctc ggccctggcc gttgcgagcg cgctcccccg cgcgatcacg
      181 gtgaggctgc cccaggcgac cgccccgccg aggcagagga agtcgcccgt gagcgagccg
      241 gcacccgcgc tccgatccga caacacgacc gcgatccccc cgagcgccac cagcagaccc
      301 agcaccttca cgaccgagag gcggtccccc ggaatgaaga aatgcgcccc gaccgcgacg
      361 aacaggggcg acgtgtagag gaacgtcaca ccgcgcgccg ccgtcgtccg ctcgaggccc
      421 ggatacagca gcgcaaactc gatcgtgaag cagagagcgg ccatcacgcc cggcagccac
      481 tggcgacccg tgagcaacac cgggatgcca cgcacgaggc accatccacc gagcagcacc
      541 agtgcacccc acgagcgcaa ggccgcctgc aggaacggtg agatgccctc gttgccgacc
      601 ttcatggcca cctgcccgat gccgagcagg atgcacagca ccgtcaacag cacgaccgcg
      661 aagagatcga gggaggactt cggcaccctc acgcgccacc gatgatctgg aaccgcggcg
      721 aggccgcgcg caacagggcg agtaccctgt cggccatgag ctcgtaagcg ggatcggtga
      781 aatggacgcc gtcacccgca cgcatcagcc gcacttgtcc cttgaggtcc ttaaaatagg
      841 cctggtagac gccgcgatcg tcggccgtga tggagcgcgt cggcatgagc cgcacatgcg
      901 ggcggcttgc cgcagccttg acgatgatgt cgttgatgag ctcggcatcg gccgtcgcat
      961 cgctcgcgcg cattatcggc agcagcagcc agacgacggg cactttgtgg cgacccgcgt
     1021 gatccatgaa gcggccgacc cgctccgtgt agagctcgat ccagcggggc gccttgaaga
     1081 gtgccttcgg gcgcccatcg atgaagaacg agcggcggtc attcgccccg atcatcatca
     1141 cgaatgcgtc gggcttgccg ccctgcgcga cggcgcgatc gagcatgtcg gtgaggtcgc
     1201 tcgcggcaaa gccgaccgag ttcttggcgc cgcgcacgat caggacgcgc ttgtcccgtt
     1261 gcagcatgcg atagagcccg ccccacatgc catccgcgag gctgtcgccg acgacggcga
     1321 tcgtgaggcg gggctcggct gcagcggtcg tccgctcagc aagcagaccg gcgcccgcaa
     1381 taccggcaag cacgacccgc cgcgacagat cctggctttc ctgcgattgc cgcatcatgc
     1441 ccccccggct cgcacggcct tacttcccat cgtccatccc tttagcagca gtgggatggc
     1501 ctggccacgc ggcattcgac cctgcgagcc tcgcggcgcc aggatgtggt cattgagcat
     1561 gcgggcggaa atctctatgc tggaccgggg aaggctctcg gcaagcgacg ctgtcagttc
     1621 cggagcaatc gcatgacccg cactctcatt gccgcatcga tgttcgcact tctcgcgctc
     1681 ttcgcgccgc cgtttctcgg cggcgaagtc gccggcctac tcagcgagcg cgcagaggcg
     1741 caaggcgtgc cctactgcgc cggcccgacg cgaagatgcg cggacggcag cgttcctttc
     1801 tgcgaccgcg gcgcctgccg gcttgcacac tcaccaaccg tcgtgatcgg ctgcctgcgc
     1861 tactcctgcc cgccgccgaa gatcgtcgga ccgatcacca cgccgatcgg cagcacctgc
     1921 gcggcccggc ccttgtgtcc gacaggaacg atcgtggcct gccggcagtt cgggcgatgc
     1981 tctgccaacg tcttcacgcg gccgacgcag ggctgcctcc gctacgagtg ccgccggctt
     2041 tgacggccga attgacgggc gaatcgaggg acgcggcgcg caggctgtta ctctctgtgc
     2101 gaatgacgca atcccagtcc tcgctccggc gcctgcaggc cgaccctgca gttcggctga
     2161 accccgacga tggcgacagt ggccctctgc tgcctgtagt ggaccaacga ttgcgccatt
     2221 gccctgcgga gtgcacgaga aagcgctcta ctagggccaa gaattaacat ccgttcatat
     2281 tgagatggag gccaccatgt tgcgagttgt cggacggtta gcctgtgctc tagctctgtc
     2341 caccccggtg atgactggtc aggcggccct tgcgctcgac tgcggaccgc tcaagcacat
     2401 cgagatcgag ctgccatccg gcgaaccatc catgatcgag cgcctctcag accgcctcgt
     2461 gaaggcgcaa tgcgcatggt ccctcaaagc gcgagcgagt gcacccggca ggctggcgat
     2521 agcttacgga acaggcgcgg aaatcgacac gctgatcgag tccgttctcg aagactttct
     2581 gccaggttac agtaaccgtg gctacgtcac ctacccgatc ggcaattcga tccgcctttt
     2641 cagacttcct gagttcggga ccacgtccac cgcgtcgacg catctgccac agcctgcggg
     2701 cggtcccgtg ctgctcttct cagtaggcct gcgcacgaac ggccagcgga tcggcatccc
     2761 gaagctcaat catctcgcag ccacggccct cgctcggaag ggcctcagaa tctggaggta
     2821 cctcgacccc aatcctgatg tcagtccggc gacgcgggtc ggtgccgatg ccttgcaatt
     2881 cgtccgctcg atccccgagc gtccgcggct cgtggtcctg acgagcgcgg cgcgagcccc
     2941 tgcgaccagt cccaatggcg atccggaggc tctggacctc agcgcccatc tccttgatga
     3001 ggtgagtggc gaatggtccc aaatctggaa ggcctcggaa agaatttcgc ccgtgagcga
     3061 gtgcacgtcg agaatacatg aatgcgaagc caaattcttg atgacgacga tctggccgaa
     3121 gctcgaaggc gcatgggccg atgagatcgc cggcaaactg aagtgactcc gacttttcga
     3181 gcagggctcg gcgcacgccg ggcggtctcg ctcgttgcac atcgacgacg ttcgcccgtc
     3241 ctgcgattga cctcgacgcg gcagcgcggg caaacttcgc accccagccg cagcgaggat
     3301 cgcgcgccat gtccgaacca gccctgaaag tcgcaaccgc gggcggcatc ctgcgcctca
     3361 cactcaaccg gcccgagaag ctcaacagca tgacgaaggc gctgggccgc gatctcgccg
     3421 cggctctcga cgcagccgct tcggatgaca gctgccgcgt catcgtgatc acgggagcag
     3481 gccgcgcctt ctgcgccggc caggacctct cagacggcgt ctacgtgaaa ggcgggccgc
     3541 aacccgacct tggcgacacg gtcgatgcct ataacccgat catcctgaag ctccgccaga
     3601 tcccgaagcc ggtcatcgcc gcggtcaacg gaatcgcggc cggtgccggc gcctcgatcg
     3661 cgctcgcctg cgatattgtg ctcgccgccc gctcggcgag cttcctgcaa gccttcgcca
     3721 agatcggcat tattcccgac tgcggcagta cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnngccgctc cccgccgaga aggcggaggc ctggggcctc
     3841 atctggcggg cgatcgacga tgacaagctc gccgccgagg tcgacgccct cgccgccagg
     3901 ctcgccatcg caccgaccta cggcctcggc ctcaccaagc aggcgcttga cgccgcggct
     3961 gacaactcgc tcgcggagca gctggcgctc gagcgccggc tccagtcgga ggccggccgc
     4021 acgcccgact atgccgaggg cgtcggcgcc tttctcgaga agcgcaagcc cacgttcacc
     4081 gggagaaaag catgagccgc tcggccgaag agacagcccg cgtcagtgcg gatgcgatgt
     4141 gggccggcga cagggcgacg caaacgctcg gcatgcgact ggagagcgtc ggtccaggca
     4201 cggcccgcat gtcgctgctc gtcaccgagg cgatggtcaa cggccacgga ctctgccatg
     4261 gcggctacat cttcctgctc gccgacagcg ccttcgccta cgcttgcaac tcctacaacg
     4321 tgcgcaacgt cgcccagaac tgccagatca ccttcatcgc gccggggaag ctcggcatgc
     4381 gactcaccgc ggaggcacgc gagcgccacc gcgccgagcg gagcggcatc tacgatgcga
     4441 cggtgcgcaa cgaggcgggc gaggtgatcg ccgagttccg aggctggagc cgctcgatcg
     4501 gcgggccgct ggtagcgtga gcgcgcctca ggcttctcgg gcgtagggga cgtagcgccg
     4561 gtctgttgcg atccagtcgc cagccgagga gccgcgcccg ccgacgagcg tgcgcagtct
     4621 ctgccagatc gtgccgtcgg cgagtgtcac gatcgtctcg accgccttga cgctccccgt
     4681 tgtcggtgca cgccatattc gcgtcacggc gcggcccgag gagatcgcct cgagaagcgg
     4741 ctggaaagag gcgaggctct cgaccggctg gcgctcaaag gcttccccgg cgatcagcag
     4801 atcataggtc tggtcggaat gcaggatcag ctcgaacggg caggccgcgc ggactcgcgg
     4861 gcgcacggca atccgccagt aggtctccgt ctccacgtct tcgacatccg ccgcatggcg
     4921 caacgtcggc acccagtagc ggagggccgc gatggtgtcg tgcagcttgg agcggtaggc
     4981 atcggtcggc aacatggtcc gtcggcatcc tcatggcggc cggcgcacca tgcggacgcg
     5041 cctgtcactt tgacaagccc cttcggcgct gatccacgcc aaaaggcgaa ggtcgcgctt
     5101 tcacgccggc cagcagtcat tcctggtgaa tgcgggccta acagcaggcg agatccggcc
     5161 atatggccga gcccgctcag cctcgcttca gtgccgcgag gtccgcgatc tcgttcgcca
     5221 tgatccgatc gtcgccgccg ccccgctcgg caaggccttt cgcgacgcct gccatgtcgc
     5281 gttgatcccg gttctggctg agcttcgcct tgccgatcag gcgcgtcacc gtgatcctga
     5341 cgccgacgat gccgcgcagc atcacgtcga tgtagctttc cggcgcatcg cttacggccc
     5401 agggcgctgc tgccccttgc tcgtgctggt tggtgagatc ggtcacgtgc gcgtggagct
     5461 gcgccttatc ctcgatcagc tctgcgcgtc cataggcgtg cacgacactg tagttccacg
     5521 tcggcacggc cttgtggttc tccgccttgc tcggatacca gttcggcgtc acgtaggcgt
     5581 cgatgcccgg atagatgagg agcacctcgc cagcgcccgc gagctccttc cagtgcacat
     5641 tggcgcgcgc gaagtggcac tcgatcacgc cgccgctgcc atcgcccttg tagaccgtcg
     5701 gcaggctcgt ggcgacgagg ccttggcctc ccgacgacac gagcgtcgca aatggacgtg
     5761 ctcgcaggtg cgcggcgagc gccgcaggct cggtcatggc atttgccggc gggacgtaca
     5821 ttgcacttcc tcaaatgaat cgcgggccgc agcaagcgcc gcgacccgca tgtgagcccg
     5881 gctcgggtct gtcgtcaggc ggcgcgcgcg aggttgcgca gcacgtatgg caggatgccg
     5941 ccgtggcgcc agtagctcat ctcgtcctcg gtatcgatgc ggcagaggac gggcaccttg
     6001 agcgtatcct tgccgtcacc ggagacgatt acgacctcga gcttctggcg cggcttcaag
     6061 gttgtaagtc ccgcgatcga gacaacctcg tcacccttga ggccgaggcc ggcccagctc
     6121 gtcccctcct caaagatcag cggcagcacg cccatgccga cgaggttcga gcggtggatg
     6181 cgctcgaagg actgcgcgac gaccgcctgc acgccaagga ggcgcgtgcc tttcgctgcc
     6241 cagtcgcggc tcgagcccgt gccgtactcg cggccggcga agatgacgag cggcacgccc
     6301 tccgccttgt actgcatggc cgcatcgtag atcgacatct ccttgcctga agggtagtgg
     6361 cgcgtgttgc cgccctcctt gccgccaagg atcatgttct tgatgcggcc gttggcgaag
     6421 gtgccgcgca tcatcacctc gtgattgccg cggcgcgcgc cgaacgaatt gaagtcggcc
     6481 ggacgcacct gccgctcgat gagatacgtg ccggcgggcc cgtccttctt gatcgcgccg
     6541 gctggcgaaa tgtggtccgt cgtgatgctg tccccgaaca taccaaggat gcgcgccttg
     6601 gacacatcgc cgaccggggc cggctccatc ttgatgtcgt cgaagtaggg cgggtttgcg
     6661 acgtaggtcg agaccgagtt ccacccgtac gtgaggcttg cgcgcgcctt gcccatgccc
     6721 gcccacttgg cgtcgccctt gaagacgtcg gcgtagcgct tggcaaaggt gtccggcgtc
     6781 accgtctcgc ggatgagctt ggcgatctcg ttcgacgacg gccagatgtc cttcaagaac
     6841 accggcttgc cgtcgcttcc cgtgccgaga ggctcggtcg cgaggtcgag gttcatggtg
     6901 ccggcgagcg cgtaggcgac gaccagcggc ggcgaggcca gatagttcgc cttcacctcg
     6961 gcgtgcacgc ggccctcgaa gttgcggttg cccgagagca cggccgccgc gacgaggttg
     7021 ccgtcggcaa tcgctttcga caccggctcc ggcagcggcc ccgtgttgcc gatgcagacg
     7081 gtgcagccgt agccggtcag gttgaagccc atggcgtcga gctcggcttg cagcccagcc
     7141 ttcgtcatgt agtcggtgac gacttgcgag cccggcgcga agctcgtctt gacccacggc
     7201 ttgaccttga ggcccttggc gcgggcattg cgcgcaacga ggcctgccgc aatcagcacg
     7261 ctcgggttcg acgtgttggt gcacgaagtg atggccgcga tcaccacgtc accgtggccg
     7321 atctcggcat tcatgccatc gaccttcgcc ttcttggcgc cgacggtcgc gcccttgacg
     7381 atctcaccga ccgacttcgc gaagccggcc ttggcatctt tcaaggtgac gcggtcctga
     7441 ggacgcttcg ggccggccat ggacggctcg acggtcgcca tgtcgagctc gaggatgtcg
     7501 gtgtagatcg gctcggcgcc ggcctcgcgc cacatgccct gcgccttggc gtaggcctcg
     7561 acgagagcga cgcgctcggc ggagcggccg gtcgccttga ggaatttcac ggtgtcgcca
     7621 tcgacgggga agaagccgca cgtcgcgccg tattctggcg ccatgttggc gatcgtcgcc
     7681 cggtcctcga gcgaaagatt gtcgaggccg tcgccgaaat actcgacgaa cttctcgacg
     7741 acgccgcgct tccggagcat ctgcgtgcag gtgagtacca gatcggtcgc ggtgatgcct
     7801 tcgcgcagct tgcccttcaa gcggaagccg atcacttccg ggatcaccat ggccaccggc
     7861 tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgccgacgc cccagccgag cacgccgagg
     7921 ccgttcacca tcgtcgtatg gctgtcggtg ccgatgcagg tatcagggaa ggcctgcgtg
     7981 gtcgagcccg actgcttcgt ccagatggtc tgcgccagat actcgaggtt cacctgatgg
     8041 cagatgccgg tgccgggcgg cacgacctgg aagttcgtga aggcggagct gccccagcgc
     8101 aggaactcgt agcgctcctt gttgcgctcg tactcgagct cgacgttctt cttgaaggcg
     8161 tcggccgtgc cgaagtgatc gaccatcacc gagtggtcga tgacgagatc gacgggcacc
     8221 aacgggttga tcttggaggg gtcgccaccc atcgtcttca tggcatcacg catcgccgcg
     8281 aggtcgacca cgcagggcac gcccgtgaag tcctggagca gcacgcgcgc cgggcggtag
     8341 gcgatctcct gtgtcgaggt cttcgccgag agccacgtca ccatcgcctt gatgtcaccg
     8401 gccttcacgg agcggccgtc ctcgtggcgg agcaggttct cgagcagcac tttgagcgag
     8461 tatggcagct tagagatgcc cgggaggccg ttcttctcgg cgtcgggaag ggagaagtac
     8521 tcgtagctct tggcgccgac cttcagcgtc ttgcggcact tgaacgtgtc gatcgaggtg
     8581 ggcgtgaggc tcatcgcgcg tctccagaga gggcgccggc cgtccggcgc gacagaacat
     8641 ggcgaggtgg cggcgacccc gaggccccgc gtccttcccc gcgcgttata tagaagccca
     8701 gacagccttc aagccgaata ggcttccact ttcggctggc cggatatgac gggctcatgc
     8761 cggcgatcag ctcgttgcgg ccggcagcgc atagtaggcc ccgtagatgg cgacgcccgc
     8821 cttgttgagg gcgccgagat cggtcgggta gccccggaac cggcctggat cgaggctcgg
     8881 aagcggaaac tcgggcttcc cggagatgat ccactcggcc atggccttcc cggccccgcc
     8941 tgccgcggcg atgcccgcgc tgaagccgac cgccagccaa acgcctggca ttccgggtgc
     9001 cggcccgagg atcggctcgc catcaggcga gaaggggatc ggaccgttga tgatgcgttt
     9061 gaggcccaca cgcgccagca tcggcaggcg ccgcatggcg gccgcgagaa tgggctcgag
     9121 gcgatcgatc tcgtccggga acagctcctg cccgaactcg aacggcgtct cgccgtcgaa
     9181 ggccgggacg gtattggcct cccagccgcc gatggcaaag cggccgacct ccggcttcag
     9241 gtagaaattg aggtcgggat ccctgaggcc cggcagcccc tttgccagct cgggtatggc
     9301 ctcggtcacc gcgaactgat ggcggagtgc tgccaccggc aggtcgagcc cgagcatgcg
     9361 gccgacggcg cgcgcccaca cgcccggcgc cagcacgaca tgctcgcagg tgatccgccc
     9421 ccgatcggtg agaaccgccg acggccgccc gcctgtcatc tcgtagccgg tcaccaggac
     9481 gccttcgaga atgcgcgcgc cgagctcacg cgcccgggtc gctagcccct gcccgaccgc
     9541 ggccgggtcg atcgtcccgt cgcccgcgac gtgtgccgcc ccttcgacgc catcaaggtc
     9601 gatcagcgga aacaggtccc gcgcctcggc cggtgtcacg atccggaagt cgaggccacg
     9661 ctcgcgcgcc ttggggagcg ccgccttgaa ctcgtcgagc cgcgccctcg aggaggcgag
     9721 ccgcagcgat ccgacgggcc gccagtcgat cggaattgtc tgctgcagcc ggtcgtagac
     9781 cgcgaccgag gcctgcatca gccgcgtgag gtcgagccgg gtccggtact gcccgacgag
     9841 gcctgcggcg tgccaggtcg aaccatgcgt gatgccgctc ttttcgagca cgaccacctc
     9901 gcgctctcca agtgcggcca ggtgcatcgc aatcgatgag ccgatcgcgc cggcgccgac
     9961 gatcacgatg cgcgcgtggg tgggaaactc accggccatc gctcgaccct ttattgccca
    10021 gtgcacccag gtaccaatcg atgaaatgct gcaggttcgg ctcgcgcgtc ctcgagtatg
    10081 gtcccggagt gtaggccggt gacgagacgc cctcctgatt ggcctcgcag atcgaccagt
    10141 cctgttcgct cgtccgctgc cagaacggca ggagccgtga gacctcgtaa tcccgccctt
    10201 cgatggcgtc ccggtggacg aaccacgaga cgaccacctc ggtggtggtc gcgtcgatgg
    10261 gcgtcaaccg cgtcgcgacg gcatgatcgt cggaggcatg ctgccagaag ttcgggaaca
    10321 cggtcgcgcg cagcgtgccc tggctccagg ccttcgcctc gcgcatgcgg cccatcaacg
    10381 gcgcgatcgg ccgaccgtcg aggctctgcg tctgccatcc cgtcatcaac ggagcacgtg
    10441 ataccctcca ccagccgccc gtcatcgccg acgaggcgac tgcgcttccg agcccgagcg
    10501 atgtgaagcg cgcctcggcg agctcggtct cacgcgcgac ctcggcggcc cggcgcggat
    10561 cgaagcgggc cgtatcgtag gtgcccgaga catattcggg gtgcgccgta tcgcagtgat
    10621 agcactcgcg gttgttctcg aagatgagct tccagttggc cttcacggtg tagcggatcg
    10681 tatgtgcgag cttggcatcg cagaagccct gatgacgcat cccggcggcg atctcgcccg
    10741 cccccgcctc gaagtccacg ggctcgggtc cgagcgccac gaacaggagg ccaccgagat
    10801 ccttgatcgc gactggccgc agaccgagca tgtccgaggc aaggccgaac tcgcgctcag
    10861 tcgcggtcct gagccgaccg tcgagctcgt aggtccaggc atgatagggg cagacgagca
    10921 gcgcggactt cccccgctcg cccgagcaga cgcgtgctcc gcgatgccgg cagacattgt
    10981 ggaaggctcg gaccacacca tctcgcccgc gcaccaaaac gatgcgatcg tcacccaccg
    11041 catacgtctt ccatgcgccg gcctcggcaa gctcgcagcc gtgcgccacg tatagccaat
    11101 gccgccgcca gatgcgatcg aggtccgctc gcagcacctc gggcgacgtg tagaacgcgc
    11161 gctcgagcgg gccactagcc ccgtacgcgg ccttgagtgc agcgatgcgc gcggcatcgt
    11221 attcgtaggc ctgcgcgacg gtcatgactg acgatcctgg cggcaaagga cgggatactg
    11281 ttccacgaac ccgccgcgcc ttgcaacgat gccgtcctcc ccggccgtcc tccccggctt
    11341 gtcgccgccg ggacaacgcg cttcacttgc cgagagatca ccgccaaacg ggagcactga
    11401 attcatgtca tcgaagccgt tcaagctggt catgctgccg ccccagaccg acgattatcg
    11461 ccgctgggcc gcagagctcg ccgcgctcgc ctttggcgtg catgtcgtcg tcagtgagac
    11521 ggccgccgat gcgctcgtcg acatcgtcga cgccgacgcg gccttcggac cgttgccgcc
    11581 cgaggtgctc gacagggccg aacgcttgcg ctggctgcag gcgccgcagg ctgccccgcc
    11641 cgcggggttc tactacccgg cccttgtcgc gcatcccgtg atcgtcacca acttccgcga
    11701 gatcttcaac gatcacatcg gcgcgcacat catggcctac gtgctcgcgt tcgcccgtgg
    11761 cctccactac tacctgcccg accacctggc caggcgatgg cagccgcggc cgctcgacac
    11821 cggcgtcgtg catctccctg aaacgaccgc cctcatcgtc ggcgtcggcg gcatcggcac
    11881 cgaggcggct cgcctctgcc gcgccttcgg catgacagtg ctcggcatcg acggccgtcg
    11941 gagcgagccg agcgagcacc tggccgagct ccacacccct gaccggctcg acgcgcttct
    12001 cccgcgtgcc gatttcgtca tcatgaccgt gccgcacaca ccggcaaccg aagggctcat
    12061 cgacagccgg cgccttgcac tgatgaagcg cagtgcctac ttgatcaaca ttggtcgagg
    12121 catgacggtc cgcctcgatg atctcgttcg ggcactcaca tccggaacga tcgctggtgc
    12181 tgcgctcgac gtattcgagg tcgagccgct gccgcccgct catcctctct ggactgcgcc
    12241 caacgtgctg atcacgccgc acactgccgg ctatggtccc tacctgcagc agcggcgcct
    12301 ggacatcctc gtcgacaact gccggcgctt cgccgagggc cggccgctgc gcaatgtcgt
    12361 cgacaaggcc gcgtggttct gacgtcgcga gttgccgcct cgaggcttga tcgcgatcaa
    12421 cgcaggcagc gccggtgggg gccagtgttc tgccaaggcc aagcacccga ggagaacggc
    12481 catgaaagca cgcgatgtga tgagcaagac cgtcgtcacg gtcactcccg acacaaccat
    12541 cggcgacgtt gcccgtctga tgagcgagaa gcgcatcagc ggtatgccgg tcgtcactgc
    12601 ggacgggaaa gtcgtcggcg tcatcagcga gagcgacctc ctgcaccgct ccgagctcgg
    12661 cacagagaaa cagcgcaact ggtggctgcg cgtgttcgcc gagcccgaca gactggcccg
    12721 cgagtacgcc aaggcccacg gcaacaaggt cgccgacgta atgacgagca atgtcctgag
    12781 cgtctccgcc gacgatgatc tcgccaaggt tgcggaaatc ctcgagcggc acaaggtgaa
    12841 gcgcctgccc gtcgtcgatg gcggcaagct cgtcggcgtc atcaccaggg gcgacctcgt
    12901 ccgtgctctg gtcggcgccg gcgtggcgaa gcccgctgcg atctccgaca gcgccgcggt
    12961 gcagcacgcc atcgagacga agctgcgcga gcagcgctgg ctcgatgcca gcatgattcg
    13021 cgtgatcgcg acggacattg aggtgcagct ctggggcttc gtcacgtcag acgaccagcg
    13081 caaggcgctg cgcgtgctcg cctccgagca tgccggcggc cgcaagctag aggatcgtct
    13141 gagcgtgagc cggctccccg tcagcggtac gtgaggtcga cccgacacag ctcggcaatc
    13201 gtcagacttc gggcggattg acagtacagg cgtgaccacc gcgagatgaa tgctgacgtg
    13261 actgatcccg cgaggccgcc catgagccga tcgacccact cgacgcccct cgtcgccttg
    13321 gatgtcgtcg tgctcgacac tgagacgact gggctcgatg cggcagcagc gcggatcgtc
    13381 caggtcggcg ccgtccggat gcgtgcaggc gccatcgttg ccggcgacac cttcgaccgg
    13441 ctcgtcaatc cgggcgtgcc gatcccgaaa gccacgaccg cgatccacgg cctcgccgac
    13501 gaagacgtgc aggatgcccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccgtt
    13621 caggcctcgc ctggccacgc cccgcctccc tcgatgtccg gcccctcgcc gagatcgcgc
    13681 tcccgacgct cgcccaatac gacctcgatc gcatcgccgc cgcgctcggg gtcgaggtcg
    13741 tcgggcgcca caccgcactc ggcgacgcca ggaccacggc tggaatcttc gcgggcctca
    13801 tcccgctgct ccgccagcgc attggccgcc tgggccagca ggcggacttc ag
//
LOCUS       DAORNH010000099        13648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3403_length_13648_cov_2.974693, whole genome shotgun sequence.
ACCESSION   DAORNH010000099 DAORNH010000000
VERSION     DAORNH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13648
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_13648_cov_2.974693"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PK264_11595"
     CDS             complement(<1..324)
                     /locus_tag="PK264_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76566.1"
                     /translation="MTTAALLAAALFDLVAAIGLVVQPFAAGRSPRALGIDAANRAVQ
                     RMQNLALAFLTAAGGILALAACWRGSLDAGHRLALGLMAGYWLFRALLHVLYWGAVTA
                     RALGWL"
     gene            complement(332..1285)
                     /locus_tag="PK264_11600"
     CDS             complement(332..1285)
                     /locus_tag="PK264_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HRD76567.1"
                     /translation="MVAIAILDDYQNVALKMADWSRLKDLHRIQVFDKPFASEAEAAR
                     ALVGFDCLGIMRERTPMSRSLLANLPNLRLIVTTGMRNAAIDLAAAAEQKITVCGTES
                     GQSATAELAFGLILSLARSIPYEAIAMRQGAWQTTLGLELRGRTLGLLGLGKLGSQVA
                     RFGQAFGMPVIAWSENLTDEKAKAAGVERVTKDELFRRTDVLSIHTVLSPRTRGIVGV
                     RELGLMKPSALLVNTSRGPIIDTDALLATLKAGKILGAALDVYDVEPLPANHPLRSEP
                     RALLVPHIGYVTEETYRLFYGGMVEALEGWLAGKPVRVLKA"
     gene            1430..3961
                     /locus_tag="PK264_11605"
     CDS             1430..3961
                     /locus_tag="PK264_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HRD76568.1"
                     /translation="MGSEFGRRVPGAQTASPVSDLIAATRGRDDASNLARWWRGAVQL
                     AASLLIGFGIILWIAANWDDIPKFYRFAIVGGALALMVLAAFLARIRVPALVGAFLAA
                     GGLFALIGQTYQTGADPWQLFAVWAVLTLPWALAARHDLLWCCWVVVALVALVLWHAT
                     YAGGFMSSRNLGVTLSVWALATVIAVMLSPWSLIRQWIGESSWAFRIAYVLAGSIILQ
                     GALSGAFAGRSIGSAYFLGLIALGGAAAALYALKPFEFFLLALTALGIDALLITGLAR
                     GILVASRWDMGSTLFIGLMAAAIVSGTGSLLVRIARQNASEVPGVGQLSQSMWPVALL
                     SGFGALLAAIPLISFLGFIVSGLLMRGPGTYIVGAAVLAAGCYAIRSGSQTSFLHQLG
                     VIGLVVGLLLLGFGVYRDLGSHGAIASLIMAAITMGLAAVIGRTWVQAILGMATAIFI
                     GAFAVQLGISDGRASPFRYLRFATGLIATLASAAIVLPVIAKDFAAPIATALEHFREF
                     IGGWAAAALLASILLAGPTFLIGGALGWRAPVTGTPVIGTTSLATLNVGSALLAAIGV
                     GLLHAFRPWMRTILGIGAAVIAVIMAAVIDGLGGLILLGACAILAGRTAISIGAVIGS
                     AWVIGAFYYWLGWPLIDKAKFMFAAGILLAAIAWVSGLVHAPKRLAFPHVEARRSAAP
                     LIAAGAVAIAALAGHSVWSKEAIITTGRQIYLALAPVDPRSLMQGDYMALRFVLPSAK
                     ELRERPEPGSVLYAVARLDDRGVAEIARVSDAYPSLAAGEVAILLRAKGGHWIVSTDA
                     WYFKEGTGKKWEAARFGVFRVGSDGTALLINLVDKDLQAIKPEIK"
     gene            complement(3995..5161)
                     /locus_tag="PK264_11610"
     CDS             complement(3995..5161)
                     /locus_tag="PK264_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRD76569.1"
                     /translation="MSKPLLISPITLRETTAKNRIMISPMATYSAVDGLANDWHLGHI
                     ARLAMGGAGIVCIEATAVTPEGRITNGDTGLWSTAHVAPMKKLADFIRSWGGIPAIQL
                     GHAGRKGAMQRPWHGNGAITPADIARGDRIWSIVAPTSDPMAPGHLVPSALSVADIAR
                     LKQAWADAAKRALDAGFDIIEIHNAHGYLVHQFLSPLSNKRNDAYGGDLQGRMRFPVE
                     LAAAVRAVVPMSKPLFLRISAVDGLDGGWTIEDSVTYARALKDVGIDVIACSSGGLTG
                     SATAARVKRTWGYNVPYAAKVRKEAEIATVAVGLIIEPEHAERILQAGEADIIAIGRE
                     ALVNPSWPHMAELALGRPAMEQLGDLPEKYGWWLKLREKQMQDIRAEDARAAGR"
     gene            5396..>5869
                     /locus_tag="PK264_11615"
     CDS             5396..>5869
                     /locus_tag="PK264_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD76570.1"
                     /translation="MSAPRLRTETADGISWIIADSPGRLNAYTRDMWEALPGAIRTAE
                     LDPAVRVIVLRGAGDQAFSAGADISEFAINRVGAAARRYDEINHEAFTAVARCGKPTI
                     AMVHGFCFGGGCELAICCDMRWVAEDAVFSIPAAKLSIGYNPRWIKPMLSVLSAAK"
     assembly_gap    5870..5905
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5906..6184
                     /locus_tag="PK264_11620"
     CDS             <5906..6184
                     /locus_tag="PK264_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD76571.1"
                     /translation="SAAEALRMGIASEVVGKAALEAETRKLAAEISANAPLSIVAAKR
                     SIDAMVDQPESVDMAMLDKLVEACFESADYTEGTRAFLEKRKPRFEGR"
     gene            6188..7084
                     /locus_tag="PK264_11625"
     CDS             6188..7084
                     /locus_tag="PK264_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD76572.1"
                     /translation="MAAPPVIFDRNLIRHRRDRAARLGPSDFLLKEAASDLADRLVFI
                     KRRFERGLVLGGYNGELARRLAGHPSIGSLATVELSPRLIEACPPPRIRADEETMPLA
                     PASLDLVVSALSLQLVNDLPGTLLHVREALRPDGLFIAALLGGRSLHELRAAFIEAES
                     ELRGGASPRVAPFADVRDLGALLQRAGFALPVADSDTLTVAYETPLHLMRDLRAMGAS
                     NPLAERSRVPVTRTLVFRAADIYQRRFARADGKVLATFEIVALTAWAPDVSQQKPLAP
                     GSARTRLADALGTAEERLPRRT"
     gene            complement(7166..7432)
                     /locus_tag="PK264_11630"
     CDS             complement(7166..7432)
                     /locus_tag="PK264_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76573.1"
                     /translation="MAMTHGSQLNELWTTTLTRLAGDASIATLARDTKAFVRPCEIKS
                     AVDLLRIILAYCLGGMGLRSTSTWAASVGLADLSDLLRAVLAQA"
     gene            complement(7501..8373)
                     /locus_tag="PK264_11635"
     CDS             complement(7501..8373)
                     /locus_tag="PK264_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD76574.1"
                     /translation="MLTPEQVDFYNEHGYVMVPDVLSPREVDELRRTVEGIVAGAASV
                     TSHTDVYDLEDGHTAAQPKVRRIKTPHKHFDYFKRLVRHQKITSALAALLGPNIRLHG
                     SKLNMKSAGYGAPVEWHQDWAFYPHTNDDVTATGILLDDCKEENGPLLVIPGSHRGRT
                     YDHHSDGAFCGAITGELNQIDFSKAVPLTGKAGSMSIHHARLLHGSAQNTSDKQRRLL
                     LFEYTAADAWPLLGVTNFQEFNERMVLGEPTVAPRIQPVPVRMPFPPASSQGSIYENQ
                     RGAKVKYFGTQKVA"
     gene            complement(8454..9743)
                     /locus_tag="PK264_11640"
     CDS             complement(8454..9743)
                     /locus_tag="PK264_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD76575.1"
                     /translation="MDLTILIMFAVFGVLIALEVPIAFALAGSALLYLVWNPVVPLSI
                     VVQRMAPGIDSFPLLAVPLFILAGQLLNTSGIATRIFRFAHAVVGHIRGGLAHCNIVA
                     SMIFSAMSGVAQADAAGLGLIEIKAMRERGFDPAFAAAVSASSAIIGPIIPPSVIMVI
                     YGLLAQVSVADLFLAGILPGIMMGLTLMGMVWLLAVTGRVHCPVEPRATYPELKAAFF
                     GGIPAFLAPIVLVGGLVFGVATPTELGALTVVYAIALGFWYGELTWRQVFEAASETLI
                     FYGVLVFIISAAVPFGWLISFTRLPAELAGVVLAYTKDPMVVLAIINVLLLFLGCFME
                     TTAILLIVTPTLLPVILAVGIDPVHFGIVMCLNLIIGAITPPFGVILFILKDIAQVSF
                     GQLVRAIMPFYIPLAVTLLIITYWPAFVLFVPKLLKG"
     gene            complement(9755..10318)
                     /locus_tag="PK264_11645"
     CDS             complement(9755..10318)
                     /locus_tag="PK264_11645"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD76576.1"
                     /translation="MLAVLDLYRGLLRLVGKLERMLGVACIAVMVSTITTQVFTRYVL
                     SRPIIWVEELATYSFIWGAFLGASLGLKHGRHVRIETFVDHLPERARSALRLLVNLVI
                     LAVLWHLVREVGKVINIESRSSSVSLPWPVPRAWFYSIPLAVSCVSMTATCAYFALVE
                     TCGLLGVSGAGQRRHVMGAPPPAGVQT"
     gene            complement(10418..11392)
                     /locus_tag="PK264_11650"
     CDS             complement(10418..11392)
                     /locus_tag="PK264_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD76577.1"
                     /translation="MKFFRGLALALGILALPAAANAQQTLTFGGSDAIGSLLDRQNAL
                     FAKLVNERAAGKLKINFIQGEQLGNDQQVIEQMIAGSVHIYGDVLDWYANWVKDFAIM
                     GWGFTFRDTDHLQKFTESAAYQKLAEELRAKQGIRILAAAATQPRVLFANKKIAGLAD
                     ISGIKMRVPEIKVYVKLWETLGARPSRLAWAEVFLGLKNGVVDAAEGPVSAAFAAKFH
                     EAVKHVMRTDHIYSSAHITINDKAFMALSPELQKIVIDAAREAVAWSRAQAARETDEV
                     LAKMAAAGATIHKIDAAAIRAKSEAAVAEMEKDGAWSAGLWKAIQDIK"
     gene            11841..12110
                     /locus_tag="PK264_11655"
     CDS             11841..12110
                     /locus_tag="PK264_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HRD76578.1"
                     /translation="MARFEVSSREMVEVAAQGGQPDALFELGMMYCSGRDVTMNLVEA
                     HKWFNLAALRGNDAAKRYRLEISSEMSKAEIAEAQRLARDWMRKH"
     gene            complement(12135..12761)
                     /locus_tag="PK264_11660"
     CDS             complement(12135..12761)
                     /locus_tag="PK264_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76579.1"
                     /translation="MLEIYGRANSINVRKVLWMCAELGLPYTRSDWGRGYRATSEAEF
                     RQVSDFGVVPVVVDDGFVLRESNTIVRYLAAKHGRADLYPADLKSRATIEQWMDWAST
                     DLYQSVRPVFLGLVVKMAPFKDQPDVIQSAGRDWNGQMQRLDRHLADSGPYVAGATFT
                     IADIPVGLVVNRWASIAFDKPKLEAVSAYYERLSQRTAYREHGRNGTP"
     gene            12831..>13648
                     /locus_tag="PK264_11665"
     CDS             12831..>13648
                     /locus_tag="PK264_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRD76580.1"
                     /translation="MPPRLRSTLVLAGFLALTVPLMPVQWLLLHISPRAARQLPSLYH
                     RALCRLIGLRRRVTGGITPGAPVLIVANHVSWLDIPLLSAIGPVTFVAKREVSAWPMV
                     GTLARLQGVIFVDRQRRTDAERTIDEIAERLIAGGVVVLFAEGTSSDGASVRPFLSAL
                     FKVVDAVRIGGAGDRVMLQTLGLAYRRVHGLAVTRAERPLVSFYGDMDMRTNALAVLG
                     TGPIEAHVSIGPPRALEALGDRKRVAALTEAEVRAAVVALNRETATLEIDEVAAP"
BASE COUNT         2243 a         4456 c         4597 g         2316 t
ORIGIN      
        1 aagccaaccg agtgcgcgcg ccgttacggc cccccaatag agcacatgca ggagtgctcg
       61 gaacagccag tatccagcca tgaggccgag tgcgagccgg tgaccggcat cgagcgagcc
      121 tcgccagcag gccgcgagtg ccaggatccc gccagcggcc gtcaggaacg caagcgcgag
      181 gttctgcatc cgctgcaccg cccggtttgc cgcatcgatg cctaaggcac gcggggatcg
      241 ccccgcggcg aacggctgga cgacgagtcc gatcgcggcg acgagatcga acagtgccgc
      301 cgccagcagc gccgcggtcg tcatgtgcgg gtcacgcctt gaggacgcgg acaggcttgc
      361 ccgcaagcca cccttccaat gcctcgacca tgccgccgta gaacaggcgg taggtctcct
      421 cggtgacgta gccgatgtgg gggacaagga gggcgcgcgg ctcgctccgc agcgggtggt
      481 tcgccggcag cggctcgaca tcgtagacgt cgagggcggc cccgagaatc tttccggcct
      541 tcagcgtggc gagcagcgcg tcggtgtcga tgatcggccc gcgcgaggtg ttgaccagca
      601 gcgccgaggg cttcatgagg ccgagctcgc ggacgccaac gatgccgcgc gtgcgtggcg
      661 agaggacggt gtggatcgag agcacgtcgg tgcgtctgaa gagctcatcc ttggtcacac
      721 gctcgacgcc cgcggccttc gccttctcgt ccgtgaggtt ctcgctccag gcgatcaccg
      781 gcatgccgaa tgcctggccg aagcgggcga cctgcgagcc gagcttgccg agcccgagga
      841 gcccgagcgt gcgccctctg agttcgagac cgagcgtcgt ctgccaggcg ccctggcgca
      901 tggcgatcgc ctcgtaaggg atcgagcggg cgagcgagag gatgaggccg aaggcgagtt
      961 cggccgtcgc cgattggcca ctctccgtgc cgcagaccgt gattttctgc tcggctgcgg
     1021 cagcgagatc gatggcagcg ttgcgcatgc cggtcgtgac gatcaggcgc aggttcggca
     1081 gattcgcgag gagcgaccgg ctcatcggcg tgcgctcgcg catgatgccg aggcagtcga
     1141 agccgacgag cgcgcgggca gcctcggcct ccgaggcgaa cggcttgtcg aagacctgta
     1201 tgcgatgcaa gtccttgagg cgcgaccagt ccgccatctt gagggcgaca ttctggtagt
     1261 cgtcgaggat ggcgatggcg accatgcggc gtggctccgt aaattaatca ttgacgcgta
     1321 cactcgcggg ctcggcctcg cggcatgcga ccatcatagc gcaaggcttc ttttgcgcct
     1381 tgtgcggtaa cgtcgccgtc cgggcagcat gcgtatcgaa gggagcacca tgggaagcga
     1441 atttggacgg agagtgcccg gggcacagac cgcatccccg gtttccgacc tgatcgcggc
     1501 aacccgcggc agggatgacg cgtccaacct cgcccgctgg tggcgagggg cggtacaact
     1561 cgccgcgtcg ctgctgatcg gcttcggcat cattctgtgg attgcggcca actgggacga
     1621 catcccgaaa ttctaccgct tcgcgattgt cggcggggcg ctggcactca tggtactcgc
     1681 cgcgttcctc gctcgcatac gggtgccggc gctggtcggc gcctttctcg ctgcaggcgg
     1741 cctcttcgcg cttatcggcc agacctatca gacgggtgcc gacccctggc aactgttcgc
     1801 tgtctgggcc gtgctgacgc tgccctgggc gctcgccgcc cggcacgatc tcctctggtg
     1861 ctgctgggtc gtcgtggcgc tcgtcgcgtt ggtgctctgg catgcgacct acgccggcgg
     1921 cttcatgagc agccgcaacc tcggcgtgac actctcggtc tgggccctgg cgacggtgat
     1981 cgcggtgatg ctcagcccct ggtcgctgat ccgccagtgg atcggggaga gcagctgggc
     2041 gttccggatc gcctatgtcc tcgcgggcag catcatcctt cagggggcgc tttctggcgc
     2101 cttcgccgga cgttcgatcg gctccgccta cttcctgggg ctcatcgcgc tcggcggtgc
     2161 cgcggcggcg ctctatgccc tgaagccgtt tgagttcttc ctgctggcgc tgaccgcact
     2221 cgggatcgat gcgctcctga tcactggact tgctcgcggc atcctcgtgg cgagccgctg
     2281 ggacatgggc agcacgctgt ttatcggcct catggcggcg gcaatcgtat caggcaccgg
     2341 ctcgctgctc gtgcgcattg cgcggcagaa tgcgagcgag gtgccgggcg tcggtcaatt
     2401 gtcgcagagc atgtggccgg tggccctgct ctcgggcttc ggcgcccttc tcgccgcaat
     2461 ccccctgatc tccttcctcg gcttcatcgt gtcggggctg ctcatgcgcg ggccgggtac
     2521 ctacatcgtc ggcgccgccg tgctcgccgc cggctgctac gcgatccgct ccggctcgca
     2581 aacgagcttc cttcatcagc tcggcgtgat cggcctcgtt gtcggcctgc tgctcctagg
     2641 cttcggcgtt tatcgcgacc tcgggagcca cggtgcgatc gcctcgctca tcatggcagc
     2701 gatcaccatg ggcttggccg cagtgatcgg gcgaacttgg gtgcaggcta tccttggaat
     2761 ggcgacagcg attttcatcg gagccttcgc cgtgcaactc ggcatctctg atggccgggc
     2821 gtcgccattc cggtatctga ggtttgcaac cgggcttatt gctacgctcg cctcggcggc
     2881 gatcgtgctg cccgtgatcg ccaaggattt tgcagcgcct atcgcgaccg cgctcgagca
     2941 tttccgcgag ttcatcggcg gttgggccgc ggcggcgctg ctcgcctcga ttctgctggc
     3001 gggcccgacg ttcctcatcg gcggtgcgct cgggtggcgc gcgcccgtga ccggcacgcc
     3061 cgtcatcggc acaacgtcac tcgcaaccct caatgtcggg tcggcactgc tggcggcaat
     3121 cggcgtcggt ctgctgcatg cgttcaggcc ttggatgcgc acgatcctcg gcatcggcgc
     3181 tgcagtcatt gcggtgatca tggcggccgt catcgacgga ctcggtgggc tcatcctgct
     3241 cggcgcatgc gcgatcctcg ccggccgcac ggcgatctcc atcggcgcgg tgattggatc
     3301 ggcctgggtg atcggggcct tctactactg gctcggctgg ccgctcatcg acaaggcgaa
     3361 gttcatgttt gcggccggca ttctgcttgc ggcgatcgcc tgggtctcgg gcctcgtcca
     3421 tgcgccgaag aggctggcgt tcccgcatgt cgaggcgcgg cggagcgctg ccccgctgat
     3481 cgctgccggc gccgtcgcca tcgcagctct tgccggccac tcggtctggt cgaaagaggc
     3541 catcatcacc acaggccgcc agatctacct cgcgcttgcc cctgtcgatc cgcgctcgct
     3601 catgcagggc gactacatgg cactgcggtt cgtcctgccg agcgccaagg agctgcgcga
     3661 gcgacccgag cccggcagtg tgctctatgc ggtcgcccgc ctcgatgatc gcggcgtcgc
     3721 cgagatcgcg cgcgtgtccg acgcctatcc aagtctggca gcaggcgagg tggcaatcct
     3781 gctccgcgcc aagggcggac attggatcgt cagcacggac gcctggtact tcaaggaagg
     3841 cacgggcaag aagtgggaag cggcgcgctt cggcgtattc cgcgtcggca gcgacggcac
     3901 ggccctgctc atcaatctcg tcgacaagga tctccaggca atcaagccgg agatcaagtg
     3961 aggcgcggcc cgctcgggcc gccgcccacc tccatcatcg cccggccgcc cgcgcgtcct
     4021 cggcgcggat atcctgcatc tgcttctcgc gcagtttcag ccaccagccg tacttctccg
     4081 ggagatcacc gagctgctcc atggcgggcc ggccgagggc gagctcggcc atgtgcggcc
     4141 agctcggatt gacgagcgcc tcgcggccga tggcgatgat gtcggcctcc cccgcctgca
     4201 ggatgcgctc ggcgtgctcg ggctcgatga tgagaccgac ggcgacggtg gcgatctcgg
     4261 cttccttccg cacctttgct gcatagggca cgttgtagcc ccaggtgcgc ttcacccgcg
     4321 ccgccgttgc cgagcctgtg agcccacccg aggagcaggc gatcacatcg atgccgacgt
     4381 ccttcaatgc acgggcatac gtgaccgagt cctcgatcgt ccagccgccg tcgagaccgt
     4441 cgacagcgga aatccgaagg aacagcggct tcgacatcgg caccacggcg cgcacggccg
     4501 cggcaagctc caccgggaac cgcatgcggc cctgcagatc gccaccatag gcgtcgttgc
     4561 gcttgttcga gagcggcgaa aggaactgat gtacgagata gccgtgcgcg ttgtggatct
     4621 cgatgatgtc gaagccggca tcgagcgcgc gcttggccgc atccgcccag gcctgcttga
     4681 gccgggcgat gtcggcgacg gagagggctg acggcacgag gtggccgggc gccatcggat
     4741 cggacgtcgg ggcgacgatc gaccagatcc tgtcgccgcg ggcgatgtcg gccggcgtga
     4801 tggcgccatt gccgtgccag ggacgctgca tcgcgccctt gcggccggca tgaccgagct
     4861 ggatcgccgg gatgccgccc caggagcgga tgaagtcggc gagcttcttc atcggcgcga
     4921 cgtgggcagt ggaccagagc ccggtatcac cattggtgat gcggccctcg ggcgtcacgg
     4981 ccgtcgcctc gatgcacacg atgccggcgc cgcccatggc gaggcgcgcg atgtgtccaa
     5041 gatgccagtc gttggcgagc ccgtcgacag ccgagtaggt cgccatgggc gagatcatga
     5101 tgcggttctt ggcggtcgtc tcgcggagcg tgattggcga aatgagcagc ggcttcgaca
     5161 tgcctggtcc cttatgcggt gtcgtggtct cgtccgagta ggccagaaca gacctgtggc
     5221 tggcaatggg gcgcggcgcg aaggcgtcat gcagcagaac tggagcccgc tggcaactcg
     5281 accggacgtc gcccgcgttt ccggaaggcg ttcatgcagc gggctcctct tgtcgcacgc
     5341 ctgccgaacc ctgtatcggt tcgaaacgtc cgaccgatga cccgggaagc tcctcatgtc
     5401 cgctccacgc ctcagaaccg agaccgccga cggcatctca tggatcatcg ccgacagccc
     5461 gggccgcctc aacgcctaca cgcgcgacat gtgggaagcg ctgccgggcg ccatccggac
     5521 cgcggagctg gatccggccg tccgcgtcat cgtgctccgg ggcgcaggcg atcaggcctt
     5581 ctcggccggc gccgacatct ccgagttcgc gattaaccga gtcggtgccg cggcccgccg
     5641 ctacgacgag atcaaccacg aggcgttcac cgcagttgcc cgatgcggca agccgaccat
     5701 cgccatggtg cacggcttct gcttcggtgg gggctgcgag ctcgcaatct gctgcgacat
     5761 gcgctgggtc gccgaggacg ccgtcttctc gatccctgcg gccaagctgt cgatcggcta
     5821 caacccgcgc tggatcaagc cgatgctctc ggtcctctcc gcggccaagn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnntccgc cgccgaggcg ctgcgtatgg gaattgcgag
     5941 cgaggtcgtc gggaaagccg ccctcgaggc cgagacgagg aagctcgccg ccgagatctc
     6001 ggccaacgct ccgctctcga tcgtcgccgc caagcgctcg atcgacgcca tggtcgacca
     6061 gcccgagagc gtcgacatgg ccatgctcga taagctcgtc gaggcctgct tcgagagcgc
     6121 cgactacacc gagggaacac gcgccttcct cgagaagcgc aagccccgct tcgaaggacg
     6181 ctgaacagtg gcggcgccgc ccgtcatctt cgatcgcaat ctcatccggc accggcgcga
     6241 ccgcgcggcc cgcctcggcc cctcggattt tctcctgaag gaagcggcct cggacctcgc
     6301 cgaccggctc gtgttcatca agcgccgctt cgagcgcggc cttgtgctcg gcggttataa
     6361 cggcgagctc gcgcgtcggc tcgcggggca tccttcgatc ggctccctcg cgaccgtcga
     6421 gctgtcgccg cgcctcatcg aggcatgccc gccacccagg atccgcgccg acgaggagac
     6481 gatgccgctc gcgccggcct cgctcgatct cgtcgtctcg gctctctcgc tgcaactcgt
     6541 gaacgacctt ccaggtacgc tgctgcacgt gcgcgaggcc ttgcggccgg acggcctctt
     6601 tatcgccgcg ctgctcggcg gacgctccct gcacgagctc cgcgcggcct tcatcgaggc
     6661 cgagagcgaa ctccgcggcg gcgcaagccc gcgcgtcgcg ccgttcgctg acgtgcgcga
     6721 tctcggcgcg ctcctgcagc gggcgggctt cgcgcttccc gtcgccgaca gcgatacgtt
     6781 gacagtcgcc tacgagacgc cgctgcacct gatgcgcgac ttgcgcgcca tgggcgcgag
     6841 caaccccctc gccgagcgct cgcgcgtgcc ggtgacgcgc acactcgtct tccgcgccgc
     6901 cgacatctac cagcgccgat tcgcccgcgc cgacggaaag gtgctcgcca ccttcgagat
     6961 cgtagcgctg accgcctggg cgccggatgt gagccagcag aagccgcttg cgccgggcag
     7021 cgcgcgcacg cgcctcgcag atgcgctcgg cacagcggag gagcgcttgc cgcggcggac
     7081 gtgaactcgc tcaacgagcg gactgccgcc cgcgcccgct cgggggatgc ctcccccgaa
     7141 ccccgtgcgt ctgcgtgttg cgtcgtcagg cttgtgccag cactgctcgt aggaggtcac
     7201 tcagatccgc caacccgacc gacgccgccc acgtgcttgt tgagcgcagc cccattccgc
     7261 cgaggcaata ggcgagtatg atccgcagca aatcgactgc cgacttgatt tcgcacggac
     7321 gcacgaacgc tttggtgtcg cgtgccagcg tcgctatcga agcgtcgcca gccaaacggg
     7381 tcagcgtcgt cgtccatagc tcattgagct gcgatccatg tgtcatcgcc atgctagaaa
     7441 ttgattgggt tgccgcaggc aaggtccatc ttggcgcgta tggggcagcg cccatcgatc
     7501 ttacgcgact ttctgcgtcc cgaaatactt caccttcgcg ccgcgctggt tctcatagat
     7561 cgagccctga gacgaggccg gcgggaacgg catgcgcacg ggcacgggct ggatgcgcgg
     7621 cgcgaccgtc ggctcgccga gcaccatgcg ctcgttgaac tcctggaagt tcgtcacgcc
     7681 gagcagcggc caggcatcgg ccgcggtgta ttcgaacagc agcaggcggc gttgcttgtc
     7741 cgatgtgttc tgtgccgagc cgtgtagcag gcgcgcatga tggatcgaca tcgagccggc
     7801 tttgcccgtc agcggcaccg ccttcgagaa atcgatctgg ttgagctcac ccgtgatggc
     7861 accgcagaag gcgccgtcgg agtggtgatc ataggtgcgg ccacggtgcg agcccgggat
     7921 gacgagaagc ggcccgttct cctccttgca gtcgtcgagc aggattccgg tcgccgtcac
     7981 gtcgtcgttg gtgtgcgggt agaatgccca gtcctggtgc cactcgaccg gggcgccgta
     8041 gcccgcggac ttcatgttga gcttcgagcc gtgcaggcgg atgttgggcc cgagtagggc
     8101 cgcgagcgcc gaggtgatct tctggtggcg gacgaggcgc ttgaaatagt cgaagtgctt
     8161 gtggggcgtc ttgatgcgcc ggaccttcgg ctgagccgcg gtatgcccgt cctcgagatc
     8221 gtaaacgtcg gtgtggctcg taaccgaggc cgcacccgcg acgatgccct cgaccgtgcg
     8281 gcgaagctcg tccacctctc tcggcgagag cacgtcgggt accatcacgt agccgtgctc
     8341 gttgtagaaa tcgacctgtt ctggtgtcag catcgtcggg ttctctgctg cgatggatga
     8401 ggtgatatat cagatggcgt gaggcagcgc cagcgtcgtt tggcgcaatt gcgtcatccc
     8461 ttgagcagtt tcgggacgaa gagcacgaac gccggccagt aggtgatgat cagcagcgtc
     8521 acggcgaggg gaatgtagaa cggcatgatc gcccgcacga gctggccgaa tgagacctgg
     8581 gcgatatcct tcaggatgaa caggatgacg ccaaagggcg gcgtgatggc gccgatgatc
     8641 aggttcaagc acatgacgat gccgaaatgc acgggatcga tgccaacggc gaggatcacc
     8701 gggagcagcg tcggcgtgac gatcagcagg atcgcggtcg tctccatgaa gcagccgagg
     8761 aagaggagca acacgttgat gatggcgagc accaccatcg ggtccttcgt gtaggcgagc
     8821 acgacgccgg cgagctcggc cggcagtcgc gtgaaggaga tgagccagcc gaatggaacg
     8881 gccgccgaga tgatgaagac gaggacgccg tagaagatga gcgtctcgct cgcagcctcg
     8941 aaaacttgtc gccaggtgag ctcgccgtac cagaagccga gcgcaatcgc gtagacgacg
     9001 gtcagcgcgc cgagttcggt cggcgtcgcg acgccgaaga cgagaccgcc gacgaggacg
     9061 atcggcgcga gaaacgccgg gatgccgcca aagaaggccg ccttcagctc gggataagtg
     9121 gcgcgcggct cgacagggca atgcacgcgc cccgtcacgg cgagcagcca caccatgccc
     9181 atgagggtga ggcccatcat gatcccgggc aggatgccgg cgaggaagag atctgcgacg
     9241 gagacctgcg cgagcagccc gtaaatgacc atgatcacgc tcggcgggat gatcgggccg
     9301 atgatcgccg aggaggcgct cacggccgcg gcgaaggccg gatcgaagcc gcgctcgcgc
     9361 atggccttga tctcaatgag gccgaggccg gccgcatcgg cctgagcgac gcccgacatg
     9421 gccgagaaga tcatgctggc gacgatgttg cagtgggcga gcccgccgcg aatgtggccg
     9481 acaacggcat gcgcaaagcg gaagatgcgc gtcgcgatgc ccgacgtatt gaggagctga
     9541 ccggcgagaa taaagagcgg gacagcgagc agcgggaagc tgtcgatgcc gggggccatg
     9601 cgctgcacca cgatcgacag cgggacgacc gggttccaga cgagatagag gagcgcgctg
     9661 ccggcgagcg cgaaggcgat cggcacctcg agggcgatca ggacgccgaa gaccgcgaac
     9721 atgatgagaa tggtgaggtc caaggggctg atcctcaggt ctggacgccg gccgggggag
     9781 gagcgcccat cacgtgtcgg cgctgtcctg cacctgaaac tccaagtagg ccgcaggtct
     9841 cgacgagcgc gaagtaggcg cacgtcgccg tcatcgagac gcacgagacg gcaagcggga
     9901 tcgagtagaa ccaggcgcgc ggcaccggcc agggcagcga gacggacgag gaccggctct
     9961 cgatgttgat caccttgccg acctcacgga cgaggtgcca gagcacggcg agaatgacca
    10021 gattgacgag gagccgcaac gccgatctgg cccgttccgg cagatgatcg acgaaggttt
    10081 cgatcctgac gtggcgaccg tgcttgaggc cgaggctcgc gccgaggaac gcgccccaga
    10141 tgaaggaata ggtcgcaagc tcctcgaccc aaatgatcgg ccggctcagc acatagcgag
    10201 tgaagacctg cgtcgtgatc gtgctcacca tcaccgcgat gcaggcgacg ccgagcatgc
    10261 gctcgagctt gccgacgagg cggaggaggc cgcgatagag gtcgaggacg gcgagcatcg
    10321 cggccggaac ccttgcaatc gagtggcgcg cccacctccg gccggtcccc gagcatgggg
    10381 acggcccgag cggcggagtt gacccaggct agtccgctca cttgatgtcc tgaatggcct
    10441 tccagagacc ggcggaccag gcgccgtcct tctccatctc ggcgacagcc gcctcggact
    10501 tcgcgcggat ggccgcggca tcgatcttgt ggatggtggc gccggccgcc gccatcttgg
    10561 cgagcacctc gtcggtctcc ctggcggcct gcgcccgcga ccaggcgacg gcctcgcgcg
    10621 cggcgtcgat gacgatcttc tgcaactcag gcgagagggc catgaaggcc ttgtcgttga
    10681 tcgtgatgtg cgccgaggaa tagatgtggt cggtccgcat gacgtgcttc acggcctcgt
    10741 ggaacttggc cgcgaaggcg gcggacacag gtccttcagc cgcatcgacc acaccgttct
    10801 tcagcccgag gaacacctcc gcccaggcga gccggctcgg tcgcgcgccg agcgtctccc
    10861 agagcttcac gtagaccttg atctccggga cgcgcatctt gatgccggag atgtcggcga
    10921 ggcccgcgat cttcttgttg gcgaagagga cgcggggctg cgtggcggcg gccgccaaaa
    10981 tgcggatgcc ctgcttggcg cggagctcct cggcgagctt ctggtaggca gcgctttcgg
    11041 tgaacttctg caggtggtcg gtatcgcgga aggtgaagcc ccagcccatg atggcgaagt
    11101 ccttcaccca gttcgcatac cagtcgagta cgtcgccata gatgtgcacg gagccggcga
    11161 tcatctgctc gatgacctgc tggtcgttgc cgagctgctc gccctggatg aagttgatct
    11221 tgagcttgcc ggcggcgcgc tcgttgacga gcttcgcaaa cagcgcattc tgccggtcga
    11281 gaagcgagcc gatggcgtcc gagccgccga aggtcagcgt ctgctgggca tttgccgcgg
    11341 ccggcagcgc caggatgccg agtgccagag ccagtcctct gaaaaacttc atagcgcgtt
    11401 cctcctcggc gccgcctcag ggtcgggcga cgttccatgg tcaagatata tcagatcgag
    11461 agtggtcgcc agcgaatggc gcgggactgt cggcaggccg cgtgagagca gcctgcggcg
    11521 gtcgaaatgc cggttgggtg aacgagcggc aaacgccgtg tgaccgaacg gctaatgcgt
    11581 gcgccgtgat gggccgatcg tgccgagacg gaacactcaa tagattggtt tgtaacgaaa
    11641 aaaccggcga atgtcgggcg gattctgagt tgaatttacc agttctcatc cgttggcaga
    11701 caagcatggg ggcgacgagg gtgctctcca gcacagccgg actgaacgac atctggagag
    11761 ctcaaagcgc gtctcgaaag tgcaacgtat cgacgaaggg gcagcgatgc aggccccgag
    11821 ggaaacaggg agagagtgtc atggcccgtt tcgaagtatc gagccgagag atggtcgagg
    11881 tcgcagccca gggcggccag cccgacgcgc tgttcgaact cggcatgatg tactgcagcg
    11941 gccgcgacgt gacgatgaac ctcgtcgagg ctcacaagtg gttcaatctc gctgcgctcc
    12001 gcggcaatga cgcagccaag cgctatcgcc tggaaatttc ctcggagatg tccaaggccg
    12061 agatcgccga ggcccagcgc ctcgcccgcg actggatgcg aaagcactga gccgctcgcc
    12121 gtccatttcc cgcatcaagg tgtgccgttg cgcccgtgct cgcgataggc ggtccgctga
    12181 ctgagccgct cgtagtaggc cgacacggcc tcgagcttcg gcttgtcgaa ggcgatcgac
    12241 gcccagcgat tgacgacgag gccgacgggg atgtcggcaa tcgtgaacgt cgcgccggca
    12301 acgtatggcc ccgagtccgc gagatggcga tcgagacgct gcatctgccc gttccaatcc
    12361 cgccccgcgc tctggatcac atcaggctga tccttgaacg gcgccatctt gacgacgaga
    12421 ccgaggaaca ccgggcgcac gctctggtag aggtccgtcg acgcccaatc catccactgc
    12481 tcaatcgtcg cgcgtgactt gaggtcggcc ggatagagat cggcgcggcc gtgcttcgcg
    12541 gcgagatagc ggacgatcgt gttcgattcc cgcaggacga aaccatcgtc gacaacaacc
    12601 ggcactacgc cgaagtccga gacctgcctg aactccgcct cgctcgtcgc gcgatagccg
    12661 cggccccagt cgcttcgcgt gtaggggagg ccgagttcgg cgcacatcca gagcaccttg
    12721 cggacgttga tcgaattggc gcggccgtag atttcgagca tgtcgagcct ccttgctgcc
    12781 gcaggcatga tagacattgg cgccatgggt cagcaaagcc caatcagacc atgccgccgc
    12841 gtctcagatc gacactcgtc ctcgcgggct tcctcgcgct caccgtgccc ttgatgccgg
    12901 tgcagtggct gcttttgcac atctctccgc gtgccgctcg tcagctgccg agcctctacc
    12961 accgcgcgct ctgccgcctg atcggcctcc gccgccgcgt cacgggtggg attacgcccg
    13021 gcgcgccggt cctcatcgtc gccaaccacg tttcctggct ggatatcccg ctgctctcgg
    13081 cgatcgggcc ggtcaccttc gtcgccaagc gcgaggtgtc cgcctggccg atggtcggta
    13141 cgctcgcgcg cctccagggt gtgatcttcg tcgaccggca gcgtcgcacc gacgccgagc
    13201 ggaccatcga cgaaattgcc gagcggctga tcgccggcgg cgtggtcgtg ctgttcgcgg
    13261 aagggacctc atcggacggc gcgagcgtcc gtccgtttct cagcgctctc ttcaaggtgg
    13321 tcgacgccgt ccgcatcgga ggcgccggcg atcgcgtcat gctccagaca ctcggtctcg
    13381 cctatcgccg cgtgcacggc ctcgcggtga cgcgcgccga gcggcccctc gtctcgttct
    13441 atggcgacat ggacatgcgc acgaacgccc tcgccgtgct cggcacgggg ccgatcgagg
    13501 cacatgtcag catcgggccg ccccgcgcgc tcgaagctct cggcgaccgg aagcgcgtgg
    13561 cggctctgac agaggccgag gtgcgcgccg cggtcgtggc actcaatcgg gagacggcaa
    13621 cgctcgagat cgacgaggtc gcggcacc
//
LOCUS       DAORNH010000100        13622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3412_length_13622_cov_2.576546, whole genome shotgun sequence.
ACCESSION   DAORNH010000100 DAORNH010000000
VERSION     DAORNH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3412_length_13622_cov_2.576546"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PK264_11670"
     CDS             <1..948
                     /locus_tag="PK264_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD76581.1"
                     /translation="GHLRRYCEYGSRLCIRLLRDGRALLVTDHRIQGSDENRVAIERI
                     GEAAPRKRTLGERPMSGIRVLDLTRVIAGPVCGRTLAAHGADVLAISGPTLPFMGTLI
                     MDVGRGKRTAHIELTDPAGRETLAGLARDADVFVQGFRPGAIAGKGFSPERVAALSPG
                     IIAVSLCAYGYSGPWSGRRGFDSLVQTASGINHAEASAAGVTGPKELPCQALDHATGY
                     FMAFGAMMALIRQMREGGSWHVRVSLAATGHWLSGLGRQLAAIKGTDVPREAIGDLLE
                     ESASGFGPMSAVRHAGLLSRTPPHWTRPSMPLGSHEARW"
     gene            988..1617
                     /locus_tag="PK264_11675"
     CDS             988..1617
                     /locus_tag="PK264_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRD76582.1"
                     /translation="MSIEVIAAFIGTCVILAITPGPNMSLIIANTLAGGFRAGLATLL
                     GTLTGLSILVTIAALGMTSIMVLMSEWFDVIRWAGAIYLIVLGILQLRRWWRVRSGAI
                     EPGAVIASSGGRYVTGVLVALSNPKVLLFLGAFFPQFLNPAAPPGPQVATLAVLFVAT
                     LALADLSYTLLVAKARATIDRRRLNVLDGLAGGLLIVGGAVLAAARRPG"
     gene            1688..2290
                     /locus_tag="PK264_11680"
     CDS             1688..2290
                     /locus_tag="PK264_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76583.1"
                     /translation="MATTANLPAALKAAVEKETRGEAIRWIGRPRPTSAFAHAAPIWA
                     MGIPWSALTFSIFGVLLLAIFAGKPPKRIIPVWEYWAMGAAVVFAGSFALIGAAMLLS
                     PFHAAWTARRTAHIITDRRLITLTSGRSTSVRSFGPAEVLLIERRQHADGSGTLHVVT
                     GFKRDSDGDKIEQRETLVGVADVAKAEVLLRSLRSERRVA"
     gene            complement(2300..3190)
                     /locus_tag="PK264_11685"
     CDS             complement(2300..3190)
                     /locus_tag="PK264_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD76584.1"
                     /translation="MSRTIDWQGQRVETSELSINLARVGKGPPVVFLHGWPEFNRTWL
                     HNLPALADRFECFAPDLRGFGRTVSKVPRPAEGTPPHLLARDLKDLADALGLRRFAIV
                     SHDVGSFAAQAFALAYPERVSALFFFNCAYPGVGRRWGEFSHFGETWYQQFHQKDFAA
                     HLVGSSREACRVYFRHFLTHWCHQKAAFEPLLEDWVDNFMLPGNLQGGFDWYVGVSKF
                     RARMMRDGALPVPKITTPTYVLWGRHDPVLRHDFADKLDDYFADHALEFAEEAGHFVH
                     VEVPELANARLRAHLARHAA"
     gene            complement(3262..3819)
                     /locus_tag="PK264_11690"
     CDS             complement(3262..3819)
                     /locus_tag="PK264_11690"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD76585.1"
                     /translation="MHLSTKPILAAAAAIALGLSGALVSATDGLAKGEPEPKPKPAST
                     VDCSLAKNKNKPACRRKTSGLSDDQLYAAGYWLARSGDYTGALMYLERAENPNDPRFL
                     TYIGFSLRKLGEHDRAMAYYGRALAIDPNFTVARAYLGEAYLTKGDVVAARRELAEVA
                     SRCGTHCPEHAELVDAFTAAGVSLH"
     gene            4572..5390
                     /locus_tag="PK264_11695"
     CDS             4572..5390
                     /locus_tag="PK264_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRD76586.1"
                     /translation="MSAAVLLVLAWVPRVAIGDKPRLIAPVFALPRRAQNSVAAVAAT
                     GLAALVIWPALPASTARISLPSLPFAGSPPIEGKAQVLAGGTLRVGGQLVRLAGIEAP
                     ERDQKCALTGGRTWSCGRGAEVELSRLARGKAVKCDVKGKDEAGATLAACDAGGVDLG
                     AALVRGGHVFAVEGMFAPYTADEADAKARKVGLWKGNPERPAVARAKAWDAAKAKAPD
                     GCPIKGHVVSGARVYVLPWDPEYGRRSVQKSRGERWFCSEEEAQGAGWKLVGKG"
     gene            5574..7907
                     /locus_tag="PK264_11700"
                     /pseudo
     CDS             5574..7907
                     /locus_tag="PK264_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
     gene            7997..9433
                     /locus_tag="PK264_11705"
     CDS             7997..9433
                     /locus_tag="PK264_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD76587.1"
                     /translation="MAILADGLVVVVKRDCPTCVMVEPVLAELRRVGTPLEVITQDDP
                     AFPEGIPDRVYDEDLRLSFDLDIETVPTLLRLSGGRETARRIGWNRAEWAETAGVSSL
                     GNGLPEQRPGCGAKNVDPGVADELAIRFGRVTFKARTVEIGSFEDDIEACFDRGWSDG
                     LPVVPPTPVRVKRMLEGTRRDPKEVLGDMPPDYRPCTVEKVAINAVMAGCRPEYMPVL
                     LAAVEAVLDDAFCLHGVIATTMYIGPIVIVNGPVRHALDMNYGVNALGQGNRANATIG
                     RALQLTIRNVGGGKPGGIDRATLGSPGKLGFCFAENEEGSVWEPLSVERGVAPGRSAV
                     TLYAGYGMHGIVDQQARTAEQLTESFAAGLNAIYHPKSFPGPDAVLVVSPEHMRLYRE
                     AGWSKRRFLDEIDRLTTVDADRILTGLGGMLPGPPLSAKGTRVKKFRPNGFFVVHAGG
                     TAGLFSAILPGWSPGGANSQLVTKVVGD"
     gene            complement(9542..>9823)
                     /locus_tag="PK264_11710"
     CDS             complement(9542..>9823)
                     /locus_tag="PK264_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76588.1"
                     /translation="IVLDDMLHPGYPTLMVAVHDYLARHPEMVVLAVIDRETIVAATK
                     FVLCEASWFKRYEERFLEEFKANIWPLGADFEPHWCLVLSLDTRLAAII"
     assembly_gap    9824..9909
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9910..10310)
                     /locus_tag="PK264_11715"
     CDS             complement(<9910..10310)
                     /locus_tag="PK264_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76589.1"
                     /translation="MAEPITYPTSGIAAVDRYLESGYETVTGMSSRFAAAISSAMLRV
                     QSELGVGGPIAEIGTFEGRFFIAMAHALRDGEVALGIDLFDWPNAEVIDRFEANCRKH
                     GVPSEKRITWKADSRSMRPAELLEKLGGRRPR"
     gene            10523..12187
                     /locus_tag="PK264_11720"
     CDS             10523..12187
                     /locus_tag="PK264_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006016501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76590.1"
                     /translation="MPARSTDDANAFDYVVIGAGTAGCLLANRLSADPNARVLLLEAG
                     GSDNYHWVHIPVGYLYLMGNPRADWGFKTAVEPGLGNRALNYPRGKLLGGCSSINGMI
                     YMRGQARDYDLWRQMGNPGWAWEDVLPYFRAHEDQWAVEPSAFDGLHQRGGEWRVEQP
                     RIRWAILDAFREAAAEAGIPKTDDFNRGDNEGCGYFHVNQKSGVRWNAAKGFLKPIRH
                     RRNLVVETHALVDRIVLEPANGAGGAKLRASALVVRQKGHVRTFTARREIILAAGAIG
                     SPQIMQLSGIGAGEHLQTCGIRLIHELAAVGENLQDHLQIRCAYKVSGVPTMNEQYRS
                     LTRRAGFALEYALRRRGPMTMAPSQLGAFTRSDPSRETPNLQYHIQPLTLPKFGEPLD
                     PFPAFTASVCNLRPTSRGHVRITSPEPGAEPEIRANYLATAEDRRVAADSVRVTRRIV
                     SMPSLQKFSPVEFRPGPEIDGDEALARSAGEIATTIFHPVGTARMGSDDQAVVDPRLR
                     VRGIAGLRVIDASVMPTITSGNTNSPTLMIAEKGAAMMLEDFRKGA"
     gene            complement(12184..12573)
                     /locus_tag="PK264_11725"
     CDS             complement(12184..12573)
                     /locus_tag="PK264_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76591.1"
                     /translation="MIIWLADAIRDQTFLKVYLCVGLAIMLLPVIVLTVMYNVGIRRT
                     PGGRRLLKEQNRPGTSRSSGTAIVMARDITRGVYGEDARRLQNRTYWLFGAWLLAVVL
                     VFGALIWAQDINKRRDAQPTPATTRER"
     gene            complement(12670..>13622)
                     /locus_tag="PK264_11730"
     CDS             complement(12670..>13622)
                     /locus_tag="PK264_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRD76592.1"
                     /translation="EKARSIAADRLAAAELAHRKAAQALREAQSGVTKEREERARIEA
                     RLENARTRRVEETGRIREHLGIEPEGCLAIADVESGAAMPSLAEADRTVTRLKGDRER
                     LGGVNLQAEQELDELAEQFTKMETERADLETAINKFRGAIAQLNGEGRRRLQAAFETV
                     NGHFQRLFTTLFGGGEARLEMIESAEDPLEGGLEIIAKPPGKKPATLSLLSGGEQTLT
                     ALSLIFAVFLTNPSPICVLDEVDAPLDDSNVDRFCTLMEKMAVDTETRFLVITHHPMT
                     MSRMTRLFGVTMAEKGVSQLVSVDLQMARSIVGEEGEAAA"
BASE COUNT         2076 a         4503 c         4640 g         2317 t
ORIGIN      
        1 ggtcatctcc gccgctactg cgagtatggg tctcgcctct gcatacgcct tcttcgcgat
       61 ggccgcgccc ttctcgtaac cgatcaccgg attcagggca gtgacgagaa tcgggttgcg
      121 atcgagcgca tcggcgaggc ggcacctcgc aagcgcacac tcggtgagcg gccgatgtca
      181 ggcattcgcg ttctcgacct cacgcgcgtg atcgccgggc cggtctgcgg gcgcacgctt
      241 gcagcccacg gagcggacgt gcttgcgatc tcgggcccga cgctcccgtt catggggacg
      301 ctcatcatgg acgtcggccg cggcaagcgg accgcgcaca tcgagctcac cgacccggcc
      361 gggcgcgaga cgctcgccgg ccttgcgaga gacgccgacg tcttcgtgca aggcttccgc
      421 cccggcgcga tcgcgggcaa gggcttctcg ccggagcggg tcgccgcgct ctcgccgggc
      481 atcatcgccg tctcgctctg cgcctacggc tattcgggtc cctggtccgg ccggcgcggc
      541 ttcgacagcc tcgtgcagac ggcgagcggc atcaaccacg ccgaggcgtc agctgccggc
      601 gtcaccggcc cgaaggagct gccatgccag gcgctcgacc atgccacggg ctacttcatg
      661 gcctttgggg cgatgatggc gctcatccgc cagatgcgcg aagggggctc gtggcatgtg
      721 cgcgtgtcgc tcgccgcgac cggtcactgg ctctcggggc ttggccggca gctagcagcc
      781 atcaagggca ctgatgtgcc gcgtgaggcg atcggcgatc tcctcgagga gagcgcaagc
      841 ggcttcgggc ccatgtcggc cgtgcgtcat gcaggcctgc tctcgcgcac tcccccgcac
      901 tggacccggc cgtcgatgcc gctcggcagt cacgaggcgc gctggtagtc tcggcacgtt
      961 tgcagccatc gaagcaggca gatcgacatg agcatcgaag tcatcgccgc cttcatcggc
     1021 acgtgcgtga tcctcgccat cacgccgggg ccgaacatga gcctcatcat cgccaacact
     1081 ctcgccggcg gatttcgcgc gggcctggcc acgctgctcg gcacgctcac cggcctttcg
     1141 atcctggtca cgatcgcggc cctcggcatg acctcgatca tggtgctgat gtcggagtgg
     1201 ttcgacgtga tccggtgggc cggcgccata tacctgatcg tgctcggcat cctgcagctt
     1261 cgccggtggt ggcgcgtccg gtcgggcgcg atcgagcctg gcgccgtaat cgcgagctcg
     1321 ggcggccgct acgtcacggg agtgctcgtc gccctctcga accccaaggt gctgctgttc
     1381 cttggcgcct tctttcccca gttcctgaac ccggcagcgc caccgggacc gcaggtcgca
     1441 acgctcgccg tgctgttcgt tgcgactttg gcgctcgcgg atctaagcta cacgctgctg
     1501 gtcgccaagg ctcgcgcgac gatcgaccgg cgccggctca acgtgctcga cgggctcgcc
     1561 ggcggcctcc tcattgtcgg cggcgccgta ctcgcggcgg cccgccgtcc ggggtaacgg
     1621 cgcgtcaacc acgtgtcagg cgtgcggcat tcacagtccg cacatgtctt tgatgcaatc
     1681 tcacggcatg gcgacaactg caaatctgcc ggccgcgctc aaggccgcgg tggagaagga
     1741 gacgcggggc gaggcgatcc gctggatcgg acgcccgcgt ccgacgagcg ccttcgcgca
     1801 tgcggcaccc atctgggcaa tgggcatccc atggtccgcg ctcaccttct cgatcttcgg
     1861 cgtcctgctg ctggcgatct tcgccggcaa gccgccgaag cgcatcattc cggtctggga
     1921 gtactgggcg atgggtgcgg cagttgtttt tgccggctcc tttgcgctga tcggcgccgc
     1981 gatgctgctg tcacccttcc atgctgcctg gacggcacgc cggacggcac acatcatcac
     2041 tgaccgtcgg ctgatcaccc tcacatccgg ccggtcgacc agcgtgcgct ccttcggacc
     2101 cgccgaggtg ctgctgatcg agcgccgcca acacgccgac gggagcggca cgctgcacgt
     2161 cgtcaccggt ttcaagcgtg acagcgacgg cgacaagatc gagcagcgcg agaccttggt
     2221 cggtgtcgcg gatgtcgcca aggcggaagt gctgctcagg tccttgaggt cggagcggcg
     2281 ggtcgcctga gctgcggcct tacgcggcat gccgggcgag atgcgcgcga aggcgtgcgt
     2341 tggcgagctc cggcacctcg acgtgcacga agtggccagc ctcctccgcg aactccagag
     2401 cgtggtcggc aaagtagtcg tcgagcttgt cggcgaagtc gtgccggagc accggatcgt
     2461 gccggcccca gagcacgtac gtcggggtcg tgatcttcgg cacggggagc gcgccatcgc
     2521 gcatcatgcg ggcgcggaac ttggatacgc cgacgtacca gtcgaagccg ccctgcaggt
     2581 tgcccggcag catgaaattg tcgacccagt cttcgaggag cggctcgaag gccgccttct
     2641 gatgacacca gtgcgtcagg aaatggcgga aatagacgcg gcaagcttcg cgcgacgagc
     2701 caacgagatg ggcggcgaag tccttctggt ggaattgctg gtaccaggtc tcgccgaagt
     2761 ggctgaactc gccccaacgt ctgccgacgc ccgggtaggc gcaattgaag aagaagagcg
     2821 ccgagacgcg ctcgggatag gcaagcgcga acgcctgcgc cgcaaacgag ccgacgtcgt
     2881 gcgagacgat cgcgaagcgg cggagaccaa gcgcgtcggc gagatccttc agatcgcggg
     2941 cgaggaggtg gggcggcgtg ccctctgccg ggcggggcac tttgcttacc gtgcgaccga
     3001 agccgcgcag gtccggcgcg aagcactcga agcggtcggc gagcgcgggc aggttgtgga
     3061 gccaggtgcg attgaactcg ggccagccgt gcaggaacac gacgggcgga ccctttccga
     3121 cccgcgcgag attgatcgag agctcgctcg tttcgacgcg ttggccctgc cagtcgatcg
     3181 tccggctcat gaatcatcct cccgggaaaa gcaaaggcgg cgcaaggtgc gccgccaggc
     3241 tcagtgtcga gagtgacacc gtcagtggag gctgacgccg gcagccgtga aggcatcgac
     3301 gagctcggca tgctcggggc agtgcgtgcc gcagcggctc gcgacctcgg caagctcgcg
     3361 gcgtgcggcg acgacgtcgc ccttcgtgag atacgcttcg ccgagatagg cccgcgccac
     3421 cgtgaagttc ggatcaatgg cgagcgcgcg gccgtaatag gccatggcgc gatcatgctc
     3481 gccgagcttg cgcagcgaga agccgatgta ggtgaggaag cgcgggtcgt tcgggttctc
     3541 ggcccgctcg aggtacatga gggcgcccgt gtagtcgccg gagcgggcga gccaatagcc
     3601 ggcggcgtag agctggtcgt cgctgaggcc gcttgtcttg cgccggcagg cgggcttgtt
     3661 cttgttcttg gcgagcgagc aatcgacggt ggacgcgggt ttcggcttcg gctcgggctc
     3721 acccttggcg aggccgtcgg tcgccgagac aagggcgccg gaaaggccga gcgcgatcgc
     3781 tgcggcagct gcgaggatcg gcttcgtcga gagatgcatg cgggcactcc ttcttgacgc
     3841 tgggtactgg ctcacggggg ccggcggttc gagcggcgca ccggccgaag tactcgatgt
     3901 tcggattttc cgcgatcccc ggacgttgtg caaggcgtcg aactccgctc tgacggagaa
     3961 gtgaacttcc ccaggtgttg aaagctggcg acaaagcatc gccagaattc gtgctaagcg
     4021 tccccgtgga ctatgattac ggacgaatcg ctgcggaggc ccccggcagc aaagccagcc
     4081 cgtgccgtcg tcgcaccgtg tggcatcgac atgcccatgc gcgtgacggc ggtcccgagc
     4141 gaggagggac accatgcgtt tctggcgcaa gaagagcgaa ggcttcgagt ggcgtaagta
     4201 cgtccagacc acgatcctga tgcggcgcga gcagcgcaag gcgcaagtgc gcggcgccgt
     4261 cgccggtgcg gccgaaggca tcaaggccgc tggcaatgcc gccgcggtcc gcgccgatcg
     4321 tgggctcaag gtgtctggga gcctcgcgcg agattggctc ggcgcgagct gggagcgcct
     4381 tctagccgcg ggctcgattg ccggatccct gtcgaggcgc ggcctagaga tggcagccgt
     4441 cggtctcagg ccggttctcg acttctgtgg ccgtcctgtc gtgtcagacc ggctggtcgc
     4501 cgctgcgatc ctggtgctga tgctcggcgt cgctcgtctc atgcagctcg gcggggacca
     4561 gcctcgcatt cttgtcggca gcggttctac ttgttcttgc ctgggttccg cgtgtcgcga
     4621 tcggagacaa gccgcgcctc atcgccccgg tcttcgcctt gcctcgccgc gcacagaaca
     4681 gcgttgcagc ggtcgccgcg actggcctcg cggcactcgt catctggccg gcgctgcccg
     4741 catcgacagc gcggatatcg ctgccgtcac tgccgttcgc cgggtctccg ccgatcgagg
     4801 gcaaggcgca ggtgctcgca ggcggcacgc tgcgcgttgg cggccagctc gtgcgtctcg
     4861 ccgggatcga ggcgcctgag cgcgaccaga aatgcgcgct cacgggcggc aggacgtgga
     4921 gctgcggccg cggtgccgag gtcgagctct cccgcctcgc ccgaggcaag gccgtgaagt
     4981 gcgacgtgaa gggcaaggac gaggccggcg ccacactcgc cgcttgcgat gccggcggcg
     5041 tggacctcgg agcggctctg gtgcgaggcg ggcacgtctt cgccgtcgag ggcatgttcg
     5101 cgccctacac cgcggacgag gccgacgcca aggctcgcaa ggtcggcctc tggaagggca
     5161 atccggagcg acccgccgtt gcccgggcca aggcctggga cgccgccaag gcgaaggccc
     5221 cggatggctg cccgatcaag ggccatgtcg tctccggggc gcgcgtctac gtgcttccct
     5281 gggatcccga gtacggccgc cgcagcgtcc agaagagccg cggagagcgc tggttctgca
     5341 gcgaggagga ggcccagggc gccggctgga agctcgtcgg caagggctga gagcccctca
     5401 gtacgacgtc gtcgcgattt gtgaccggct cgccatgcag cgacgggcct tccagagcgg
     5461 cccggcaccg cctaatgtcc tccccgacac gcggagggga cgacatggca gcgccgggaa
     5521 cgacagggcc agcgccgggg agcgcaggcc cgctcaacca ggccgacatc gacaagctcg
     5581 agcaggaact cgattcggaa gtgcgcttcc gcccgctcgg gcgtatcgcc gaaatcatcg
     5641 tggccggcct gctgctcgcc ctctcgattt tccactacta cacggcgggc ttcggcctgc
     5701 ttcccgagac gacgcatcgc ggcatccaca tcgccttcgt gctcgggctg atctttcttg
     5761 tgttcccggc ccgtaccagc acgacctcct ttgccggtct ctcgagcctg ggacggaagc
     5821 aggcgatcac tgccgctgtg ctcgtggggc tcctgtacct cagcccgatc tcggtccgcg
     5881 acttctccct catgacgcgc gagcactggt ggctggttgg ggcagcgatc gtgctgcttg
     5941 gcggcagcta tctgacctcc catggtggca agcctgatgc gagcccgatc gcgcccttcg
     6001 ggatcagtct cttcgactgg gtgctgatgg ccggtgccgt ggcttcgagc ctctacgtgc
     6061 cctgggtgtt ccatgatctc caattccgcg tcggcaaccc gctgacgatc gactgggtga
     6121 tgggcacgat catggtcgtc gtgctgctcg aggcctgccg gcggagcgtc ggctggccgc
     6181 tccccatcat cgcgatcgtg ctgatcctgt atgcgctcta cgggcgctcg atgccgggca
     6241 tcctccagca tcccggcaac cagtggaaag gcgtcgtcaa ccatctctat ctgacgagcc
     6301 aatcgatctt cggcgtggcc ctcggcgtgg tggcgacgtt cgtcttccac tacgtgctgt
     6361 tcggcgtgct cgcgacgcgc atgggcctcg gccgcttctt catcgatctc gcgaccgcgc
     6421 tgacggggcg cttctcgggc ggcccggcga aagtctcgat cttcggctcg ggcctcttcg
     6481 gcatgatctc cggctcctcg atcgccaaca cggtaacggt cggatcgctc acaatcccgg
     6541 cgatgatccg catcggctat ccgcgccact tcgcaggcgc tgtcgaatcg acagcagcga
     6601 cgggcggcca gatcacgccg ccgatcatgg gggctgccgc cttcctgatg gtcgagtact
     6661 tgaacatgcc ttaccagtcg atcgttctgg ccgcgatcgt gccggcggcg atgcacttct
     6721 tcggcgtctt ctgtcaggtg cacttcgagg cgaagaagca cggcctcaag ggtctgccgc
     6781 cggaggagct gccgcgcgcc ggcgaggtga tccgacgtga ctggccgacg tcgatcccgc
     6841 tgctcgtgct gctgatggtg ctgttttccg gcttcacgcc ctaccttgcc gccttctggg
     6901 gcatcacggc ctgcatcctc atctggctga ccgggcggga cccgctgatt gccgtcgggc
     6961 ttctggtgtt ggtcggcgtc gagatcgccc tcgacctgct gacggggtgg ccgggcattg
     7021 ccgtcctctt cgcgctgatc ctcgcggcga tcgccatgaa gctctcgaag gcgccagacg
     7081 ccaagcgtca gctcctcgag ctggccgacg ctttcgtagt cggtgccaag tacgcgatct
     7141 cggtcggtgc agcggcggcg accgtcggca tcatcatcgg cgtcgtgacg ctgacgggtg
     7201 tcggcttcaa gctctcgaac atcgtgacca gcacggcggc cgagctcgcc tcgttttttg
     7261 ccacgctgct gccggcctcc ctcgccgacg tgaagggcat gacgctcttc ttcacgctcg
     7321 tcatgacggc gatcgtatgc atcctgctcg gttgcggcgt gccgacaacg gccaactacc
     7381 tcatcatggt gacgatcgcg gcgccggccc tcgccatgct cggcgtgccc cagctcgtct
     7441 cgcacttctt cgtcttctac tacggcgtgc tcgccgacat cacgccgccg gtggcgcttg
     7501 ccgcctatgc gggtgccagc atggcgggcg ccgacccgtt caagaccggc aatacagcct
     7561 tccggctcgg gctcgcaaag gcgctcgtcc cgttcgtgtt cgtctattcg ccctccatgc
     7621 tgctcgtcgt gaaggggttc acgtgggccg cattctggga gacgaccatc ggctgcatgg
     7681 tcggcattgt catgctcgcg gcggcattga ccggctacgg cctcgcgcgg ctgacgcgcc
     7741 tcgagcaggt ctggctcgca atcgcctccg tgctcgtcat cacgccgagc cgcaccgcaa
     7801 ccttcgttgg cctcgccatg gccgccccca tcctcgtccg ccagatcatg gcgtggcgga
     7861 gcggggcggc gcaggcggcg agcagcgctg ccgggacctc ggtttgagtg caatccggca
     7921 gccgcagcct ccgtattgcg atgccggcgc caatccacca tggtgggcgt cggtgagctc
     7981 aaccggaggg gcggacatgg cgatcctggc cgacggtctc gtggtggtcg taaagcgcga
     8041 ctgcccgacg tgcgtgatgg tcgagcccgt gctcgccgag ctgcggcgcg tgggcacgcc
     8101 gctcgaggtg atcacgcagg acgatccggc gtttccggag ggcattccgg atcgcgtgta
     8161 cgacgaggat ttgcgcctct cgttcgatct cgacatcgag acggtgccga cgcttctgcg
     8221 cctttcgggc ggtcgcgaga cggcgcggcg gatcggctgg aaccgcgcgg agtgggccga
     8281 gacggccggc gtgtcgagcc taggcaatgg gctccccgag cagcggccgg ggtgcggtgc
     8341 gaagaatgtc gatccgggtg tcgccgacga actcgcgatc cgtttcggcc gcgtcacgtt
     8401 caaggcccga acagtcgaga tcggcagctt cgaggacgac atcgaggcct gcttcgaccg
     8461 cggctggagc gatggcctgc ccgtcgtgcc gccgacaccc gtccgcgtga agcgcatgct
     8521 cgaggggacg cggcgcgatc cgaaggaggt cctcggcgac atgccgccgg actatcgacc
     8581 atgcacggtc gagaaggtcg cgatcaacgc cgtgatggcc ggctgccggc cggagtatat
     8641 gccggtgctg ctcgccgccg tcgaggccgt gctcgacgat gccttctgcc tccacggcgt
     8701 gatcgcgacg accatgtaca tcggcccgat cgtgatcgtg aacgggcctg tacggcacgc
     8761 gctcgatatg aactacggcg tcaatgcgct cggccagggc aaccgcgcca acgcgacgat
     8821 cgggcgggcg ctgcaactca ccatccgcaa cgtcggcggc ggcaaacccg gcggcatcga
     8881 ccgcgcgacg ctcggcagcc cggggaaact cggcttctgc ttcgccgaga acgaggaggg
     8941 ctcggtctgg gagccgctct cagtcgagcg cggcgtggca ccgggccgtt cggcagtgac
     9001 gctttacgcc ggctacggca tgcacggcat cgtcgatcag caggcgcgga cggccgagca
     9061 gttgacggag agcttcgcgg ccggcctcaa cgcgatctac catccgaaga gcttcccggg
     9121 gccggacgct gtcctcgtcg tttcgccaga gcacatgcgt ctctatcgcg aggccggctg
     9181 gtcgaagcgc cgcttcctcg acgaaatcga ccggctgacg accgtcgacg ccgaccgcat
     9241 cctgaccggt ctcggcggca tgctgcccgg gccgccgctt tcggccaagg gaacgcgcgt
     9301 gaagaagttc cgcccgaacg gcttcttcgt cgtgcacgcg ggcggcactg cgggtctctt
     9361 ctcggcgatc ctgcccggct ggagccctgg tggcgccaac tcgcagctgg tgacgaaggt
     9421 ggtcggggat tgagccggac acacatgccc gcgcggccca atctatgcgg cgaaggcgag
     9481 cacagagggc ggcaggcgaa ctggagcttt gcctcgaggg gttcagggcg gctgacgggt
     9541 atcaaatgat cgccgcgagc cgtgtgtcga gcgagagaac caggcaccag tggggctcga
     9601 agtcggcgcc gagcggccag atgttggcct tgaactcctc gaggaagcgc tcctcgtagc
     9661 gcttgaacca ggacgcctcg cagaggacga acttggtggc ggcgacgatc gtctcgcggt
     9721 cgatgacggc gagcaccacc atctccgggt gccgtgcgag gtagtcgtgc acggcgacca
     9781 tcagcgtcgg gtagcccgga tgcagcatgt cgtcgagcac gatnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnc ggggccggcg gccaccgagt ttctccaaca gctccgcagg cctcatgctg
     9961 cggctgtcgg ccttccaggt gatgcgcttc tctgaaggta cgccgtgctt gcggcaattc
    10021 gcctcgaagc ggtcgatcac ctccgcgttc ggccagtcga agagatcgat gccgagcgcg
    10081 acttcgccgt cgcgaagcgc gtgcgccatg gcaatgaaga agcggccttc gaaggtgccg
    10141 atctcggcga tcggtccgcc aacaccgagt tcgctctgca cgcgcagcat ggccgagctg
    10201 attgcagctg cgaagcgcga cgacatgccg gtcacggtct catagccgct ctcgaggtag
    10261 cggtcgacgg cggcaattcc ggaggtcgga tatgtgatcg gctcggccat ggcgatcctg
    10321 cggaggcaac gattgacgga gcgcccttag accgggtgaa ccatggcgcg gtcaaggcgc
    10381 gttttcgagc gaagcacggc atcaaccatg ctcgctacgt cacacgcgag cgcctttgcc
    10441 agcgcggcgg gttcggatat cagttgaggc tctaaatttc agccgggcca ggcgagcgcc
    10501 ggcgccggca ccggagcttc tcatgcccgc acgatccacg gatgacgcga acgcattcga
    10561 ctatgtggtg atcggcgccg gcacggccgg atgcctgctc gccaaccggc tctcggccga
    10621 tccgaacgcg cgcgtcctcc tgctcgaggc cggcggcagc gacaactacc actgggtgca
    10681 catccctgtc ggctacctct atctcatggg caatccgcgc gccgactggg gcttcaagac
    10741 cgctgtcgag ccgggcctcg gcaaccgcgc cctcaactat ccgcgcggca agctgctcgg
    10801 cggctgctcg tcgatcaacg gcatgatcta catgcgcggc caggcgcgcg attacgacct
    10861 ctggcggcag atgggcaatc ccggttgggc ctgggaggat gtgctcccct acttccgcgc
    10921 acacgaagac cagtgggccg tggaaccgag tgcgttcgac ggtctgcatc agcgcggcgg
    10981 cgaatggcgg gtggagcagc cgcgcatccg ctgggcgatc ctcgacgcct tccgtgaggc
    11041 ggccgccgaa gccggcatcc cgaaaacgga cgatttcaat cgcggcgaca acgaaggctg
    11101 cggctatttc cacgtgaacc aaaagagcgg cgtgcgctgg aacgcggcga agggcttcct
    11161 gaagccgatc cgtcatcggc gcaacctcgt cgtggagacg cacgcgctcg tcgaccggat
    11221 cgtcctggag ccggcaaacg gcgcgggtgg cgcaaagttg cgtgcgagtg ccctcgtcgt
    11281 gcgccagaag ggccacgtgc gcaccttcac tgcgcggcgc gagatcatcc tcgcggccgg
    11341 cgcgatcggc agtccacaga tcatgcagct ttccgggatc ggcgcgggag agcacttgca
    11401 gacgtgcggc atccggctca tccacgagct tgcggctgtc ggcgagaacc tgcaggacca
    11461 cctgcagatc cgctgcgcct acaaggtctc gggtgtgccg accatgaacg agcagtacag
    11521 aagcctcacg cggcgcgcgg gcttcgcgct cgaatatgcg ctccgccggc gcgggccgat
    11581 gaccatggcg ccttcgcagc tcggcgcctt cacgcgctct gacccgagcc gcgagacacc
    11641 gaacctccag tatcatatcc agccgctgac gctgccgaag ttcggcgagc cgctcgaccc
    11701 gttcccggcc ttcacggcga gcgtgtgcaa tctgcgcccg acgagccgcg ggcatgtgcg
    11761 catcacctcg ccggagccgg gcgccgagcc ggagatccgc gcaaactacc tggcgaccgc
    11821 cgaggatcgc cgggtcgcgg ccgattccgt gcgcgtcacg cggcggatcg tctcgatgcc
    11881 gtcgctgcag aaattctctc ccgtcgagtt tcgccccggt ccggagatcg acggcgacga
    11941 ggctctggcg cgctcggccg gcgagatcgc gacgacgatc ttccatcctg tcggcacggc
    12001 gcgcatgggc tcggacgatc aggccgtggt cgacccgagg ctgcgcgtgc gcggcatcgc
    12061 ggggctgcgc gtcatcgacg cctcggtgat gccaaccatc acctccggca ataccaactc
    12121 gccgacgctg atgatcgccg agaagggtgc tgccatgatg ctcgaggatt tccggaaggg
    12181 cgcctaacgc tcgcgcgtcg tcgcgggcgt cggctgcgca tcgcggcgct tgttgatgtc
    12241 ctgagcccag atcagcgcgc cgaacacgag gacgacggcg agcaaccagg cgccgaacag
    12301 ccagtatgtg cggttctgaa ggcggcgtgc atcctcgcca tagacgccgc gtgtgatgtc
    12361 gcgcgccatg acgatggcag tgcccgagct cctgctggtc ccaggccggt tctgctcttt
    12421 cagaaggcgg cgaccgcccg gcgtgcgtcg gatgccgacg ttgtacatga cagtcagcac
    12481 aatgaccggc agcagcatga tggcgaggcc gacgcagagg tagaccttca gaaatgtctg
    12541 atccctgatg gcatcggcaa gccaaatgat catgggcggc ggccccggtt ctggggtggc
    12601 gggcggagca tgggcattga gcacgctgaa ttgccaagtg gagacggagg agtgcgacta
    12661 accgcccagt tacgccgccg cctcgccctc ctcgccgacg atgctgcgcg ccatctgcag
    12721 gtcgacggag acgagctgcg agacgccttt ctcggccatg gtcacgccga acaggcgggt
    12781 catgcgcgac atggtcatcg ggtgatgcgt gatgacgagg aagcgcgtct cggtgtcgac
    12841 ggccatcttt tccatcagcg tgcagaagcg gtccacgttg ctgtcgtcga gcggcgcgtc
    12901 gacctcgtcg agcacgcaga tcggtgacgg attggtgagg aacactgcga agatcagcga
    12961 gagcgccgtc agcgtctgct cgccgcccga gaggagcgag agcgtcgccg gcttcttgcc
    13021 cggcggcttg gcgatgatct cgaggccgcc ttcgagcgga tcctcggcgc tctcgatcat
    13081 ctcgaggcgg gcttcgccgc cgccgaacag cgtcgtgaag aggcgctgga aatggccgtt
    13141 gacggtctcg aaggctgctt gaagccgccg ccgaccctcg ccgttcagct gcgcgatggc
    13201 accgcggaac ttgttgatcg cggtctcgag gtcggcccgc tcggtctcca tcttggtgaa
    13261 ctgctcggcg agctcgtcaa gctcctgctc ggcctgcagg ttgacgccgc cgaggcgctc
    13321 gcggtcgccc ttgaggcgcg tcacggtcct gtcggcttcg gcgagcgagg gcatggccgc
    13381 gcccgactcg acgtcggcga tggcgaggca gccttcgggc tcgatgccga gatgctcgcg
    13441 gatgcggccg gtttcctcga cgcgcctcgt gcgggcattc tcgagacggg cctcgatgcg
    13501 ggcgcgctcc tcgcgttcct tggtgacacc gctctgcgcc tcgcgaagcg cctgcgcggc
    13561 cttgcgatgc gcgagctcgg ccgcggcaag ccggtcggca gcgatcgagc gcgccttctc
    13621 gg
//
LOCUS       DAORNH010000101        13608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3417_length_13608_cov_2.830739, whole genome shotgun sequence.
ACCESSION   DAORNH010000101 DAORNH010000000
VERSION     DAORNH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13608
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3417_length_13608_cov_2.830739"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PK264_11735"
     CDS             <1..1046
                     /locus_tag="PK264_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRD76593.1"
                     /translation="RNTQLIIQQESGTCRTIDPWGGSYYVERLTHDLAAKALAHIEEV
                     ERLGGMAKAIAAGIPKMRIEEAAARTQARIDSGRQTIVGVNKFKVEGDAAIEVLRVDN
                     KAVREAQVAKLERLRGERDPAAVKAALAALTAAATGGGNLLEAAVNAARLRATVGEIS
                     DACEAVYGRHRAEIRAVSGVYRSESGAMSKEIERVQAMVKAFETNDGRRPRILIAKIG
                     QDGHDRGQKVIATAFADLGFDVDIGPLFSTPDEVARQAVENDVHVIGVSSLAAGHLTL
                     VPELKAALDKEGRGDILIVVGGVIPPADYDALLAAGAKAVFGPGTNIPAAAADLIAKL
                     NAALGYQPKAAAE"
     gene            1072..1647
                     /locus_tag="PK264_11740"
     CDS             1072..1647
                     /locus_tag="PK264_11740"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD76594.1"
                     /translation="MNARPSLAVDKATFWRFVERDPEGRYEYSRGLIVQQMPGGTLRH
                     SLIAKRIAALVEGQLDPTAWVVIENNRAVETAESIRYPDVVVERLPADLDSRQTLVPL
                     MIVEVLSPTSRVIDLGDKVDEYLQIAALQVYVVADQSLARVRVWLRGADGHFPSEPTI
                     IEGLESALTVSSLGIVVPLAAIYDGLLTGRG"
     gene            complement(1680..2270)
                     /locus_tag="PK264_11745"
     CDS             complement(1680..2270)
                     /locus_tag="PK264_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76595.1"
                     /translation="MTRSLTYLALLAVATVALALSGCGESTPTAAKDKAAGSTDALAL
                     EPKEVRLTEADVRRYMAHAKEEIEVRRPYTAMKPGQIDEFMANLKAVQKKHGFDTADG
                     YSISWTIASIFGGIDHQGSKRYVSRQDRLKKQLADIRGSPYMDADSKKAAAQAIEDEL
                     AEIAAGKGQPEPRMPENIALVERFAPEILAVIVGGK"
     gene            2494..3060
                     /locus_tag="PK264_11750"
     CDS             2494..3060
                     /locus_tag="PK264_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76596.1"
                     /translation="MLDNAGRQIGYRLSAKTGSKFELRRAVTILYPSGQLMVTCPNCP
                     AERAPMIETLLFDSAAKDFNAPEKHPPAYRFELLNDEAKAANTVTISEPLTVEIGPLK
                     GWAAAIELVHKTTAGQRSAFVYSYVHDNCLAGFFMFTFDIDAKSHWLPQSELIAEFKA
                     ALASFSIETFDPASDPAVAAELKKKLGK"
     gene            complement(3077..4324)
                     /locus_tag="PK264_11755"
     CDS             complement(3077..4324)
                     /locus_tag="PK264_11755"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRD76597.1"
                     /translation="MWAPTRLSLPTLESLGAWWPRVAALARYGTDGYPPAVRRRLMIL
                     NLFAYLIAIFTFGYSLQHLFVDYRTFAPVIWINFALAASAALVPFMHRFGEIAGALLI
                     SAAELLGLLLLGAYLGSPSGVHIQYIIGAAAPFFVFGLSRRWLSLALVAICLVMHLAV
                     WVLFPAPQPEFAAHPAFIRSIYVSAIVTTFAITAAIVHYAFRLAEAAEAETDALLRNI
                     LPEPIVERLKANPGVTIADSFSDASVLFADMRGFVPVALSLGPARTVELLNRLVRELD
                     ALAAAHGVEKIKTIGDAYMAAAGVPEPQPEHALRLARFGLAMHGVIGRLAAEVGKPLE
                     LRVGIASGPLLAGVIGVRRLTYDVWGDTVNLAARLESNGVPGRVQVSARTRELIEAHF
                     KLEARGEIEVKGLGAQPVWLIAA"
     gene            complement(4401..5789)
                     /locus_tag="PK264_11760"
     CDS             complement(4401..5789)
                     /locus_tag="PK264_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRD76598.1"
                     /translation="MGNLILWLVIGYILVQFAIGVLVSRSIRNETDYILAGRSLGVGL
                     AGFSVFATWFGAETVTGSAGSVFKSGLSGAQGEPFAYAVGIILMGLFVAGRLWRAGIT
                     TFGDFFRNRFSPTVEVVVAVLLVPGSVLWAAAQIYGFGQIVASVANIDTRTAITIGAV
                     TVVAYTVVGGLLADAWTDVIQGIVIIVGLLMLLFFVLAKAGGFSAGLAAIEPARWSFM
                     VADQPWLEFIEQWAVPICGSLVAVELISRILGARSAGVASTAAISGGVLYLLVALIPV
                     VFGLLGPKLLPSLPAEQSEQLVAELAKAYLPAVLSAVFIGALISAILSTVDSALLAAG
                     SLVSHNILLRLNPDTSDRGKVVTTRVTVAVLGATAYVIALAATSIKELVELASAFASA
                     GVFVALIFGLFTRFGGPQSALAAILAGAVIWGWGRFVFELKTPYLAGLGAAVLAYVVV
                     AVLERRDQRLEA"
     assembly_gap    6194..6203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6204..7940
                     /locus_tag="PK264_11765"
     CDS             <6204..7940
                     /locus_tag="PK264_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76599.1"
                     /translation="YEACQARDEAAFRTAIEGVTVRGLRKGLAGLDFPAVVLHEWRRN
                     GMDAIMDGEVEKVAAQLRSESGWFDMWGTLFSKDKATRIATEAAERVYRSDAVRKGFE
                     TLANAIGREVGERMEIASADAAEPATQCLVAFVGPRYGSTIARIVGRDATKDMSGASD
                     KGKAAVSTGSLMMEGAGGIAGAIVLLVRRQLSNMGARIGQRVVGAVLGRVIAVVAGGV
                     GLVLIAKDVWDFRHGVIPIVANEMTSAETKEKVRVEIARASYEQISEHMQEIGSKAAD
                     RIIEIWHEFRRAHAKVLEIAERSPAFKAFLESIRPDRLSRLDEMVGILLLAEGEAGVV
                     KRLENGTLNRAVNQLEPAAFEIARDTKSLDAALQWADLAPEQLARVVENELHRKADPK
                     EFTKAALTRILALPDRLAISRLAGLKTKARDPLMELDDQRLVGLARAFDTGELDTLSR
                     YLTGLDREAGQRVLIAVANTPAKMALLSRPSVRDAILSSRNQSAAIGMMLRADGIFDV
                     GMFVEDLSLVRHGLVSPWLIWERYPYALPGLGVLGLVLLLAIWRALFGRRRAATVIVQ
                     APAGSSDKRGAA"
     gene            7937..8251
                     /locus_tag="PK264_11770"
     CDS             7937..8251
                     /locus_tag="PK264_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HRD76600.1"
                     /translation="MRIVSTEHVEDRLIEKGEFVYAVAISGANIIRDMRETITNTIGG
                     HMTRYEDLCNQTLDRALASLKEKAAATGYDGVLGVRIEHPAITQGAVSVVVFGTGFRY
                     RD"
     gene            8335..9126
                     /gene="ccmC"
                     /locus_tag="PK264_11775"
     CDS             8335..9126
                     /gene="ccmC"
                     /locus_tag="PK264_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HRD76601.1"
                     /translation="MTQQTFTQSLANPTRFMALSAALLPWITGAAGLVLAVGLVWAFG
                     FTPPDYQHKETVKIMFVHVPAAWIGMGAYTLIAVSSVGLLVWRHPLADVSAKAAAPIG
                     AAFTLVCLVTGSLWGMPGWGTYWEWDARLTSVLLLFFLYLGLIALRTSIEDESLASRL
                     TAILGIVGVVLLPIIKFSVDWWWTLHQKASVMTLGEQKVHWSIRAPLLVMALGFLLAF
                     IAMHLKAMQNEVLRRRIKAIRIARVAAETRRGASIDAASGRQPAE"
     gene            9131..9319
                     /gene="ccmD"
                     /locus_tag="PK264_11780"
     CDS             9131..9319
                     /gene="ccmD"
                     /locus_tag="PK264_11780"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HRD76602.1"
                     /translation="MASLGQHESYIIASYAVTFIILCAVVAWLVIDGRRQRELLARLE
                     ASGGRRGGRTSVGDKGAA"
     gene            9337..9930
                     /locus_tag="PK264_11785"
     CDS             9337..9930
                     /locus_tag="PK264_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HRD76603.1"
                     /translation="MGERGTPAPRRRVPIAVVPVAIFALMAMLFAFALGKGDSSRLPS
                     ALIGKAAPVMQLAPVDGLSDGVRPVPGIAPADLAKGDVVVVNFWASWCPPCIDEHPLL
                     VELASETKVRMLGINHKDTAAGARRFLGRYGNPFAGVGTDPDGRFAIEWGVYGMPETF
                     IVDGKGRVVYKHVGPITRQSLREKIIPAIEVARRAGS"
     gene            complement(9927..10277)
                     /locus_tag="PK264_11790"
     CDS             complement(9927..10277)
                     /locus_tag="PK264_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76604.1"
                     /translation="MRKMLFVLLGLVLIGGAIGNFAYRSLYSTERARTALSAPSAGAP
                     ADRGRSYIPERPPTSRTTTGTPGSGGGLMSGLWGNFEMVINILNVLVGILGIWMTMIG
                     MRLQRQALRQDQRR"
     gene            complement(10427..10888)
                     /locus_tag="PK264_11795"
     CDS             complement(10427..10888)
                     /locus_tag="PK264_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRD76605.1"
                     /translation="MARSTKDGDDDAEADGSPAGTQRLDKWLWYTRLIKSRTLAASLV
                     SEGKIRVNKERITKPAQAIRPGDVITASIQRTVRIVKVVALGARRGPASEAQTLYQEL
                     DTSGQPLAAQEAEPREKSEAPPMLPPERPRGMGRPTKRDRRLLDTFHGRRR"
     gene            complement(10903..11934)
                     /locus_tag="PK264_11800"
     CDS             complement(10903..11934)
                     /locus_tag="PK264_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRD76606.1"
                     /translation="MATERMTAAALEDLVTRALVASRTSEANARSVARALVAAEIDGQ
                     GGHGLSRVQSYAAQSRSGKVDGFATPLLTRTRSGSAMVDVAGGFAYPAFDLAIEALPV
                     MVHEAGIAAAGFVRSHHFGVVGRHVERLAEQGLVALAFGNTPGAMAAAGGRRPVFGTN
                     PIAIAAPQQEGAPLVVDLALSEVARGRIMAAAQRGEAIPEGWAFDAEGRPTTDAKAAL
                     AGSLNPSGGTKGAALALMVEVLAVALTGANPSHAASSFFDGDGPPPGVGQLLVAIDPD
                     AFGARVTFLERLSSITGAMLADPGVRLPGMRRLERRAAHAAAGTVGVEVKMLEALKSL
                     ASADRHASR"
     gene            complement(11934..12710)
                     /locus_tag="PK264_11805"
     CDS             complement(11934..12710)
                     /locus_tag="PK264_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76607.1"
                     /translation="MELGIKGLRVLVTAGAGGIGLEITKAFLSEGARVHVCDVDTAAM
                     ATLAKSHPAVTQSVCDVANRGEVAALFKDALAKLGGLDCLVNNAGIAGPTGPVHEINP
                     EDWDRCLEVCITSQFNCTRLAVEPLKASKNGSIINLSSAAGKFGFPNRSPYAAAKWGV
                     IGFTKTIAMELGGFGIRCNAILPGTVEGDRIRRVFEAKAQVAGKSMQEIKTAALANVS
                     LKTMIAPEQLADMIVYLASPRGRTISGQAISIDGDMQALV"
     gene            13332..>13608
                     /locus_tag="PK264_11810"
     CDS             13332..>13608
                     /locus_tag="PK264_11810"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD76608.1"
                     /translation="MTRRKKPEELRSHRWFGVSDLRSFGHRSRLRQMGFDADDWQGRP
                     VIGIINTWSDMNACHAHLRARVEDVKRGVLQAGGFPVELPAISLAEPM"
BASE COUNT         2349 a         4507 c         4541 g         2201 t
ORIGIN      
        1 cacgcaacac gcagctcatc atccagcagg aaagcggcac ctgccgcacc atcgatccct
       61 ggggcggctc ctactacgtc gagcggctga cgcacgatct cgcggcgaaa gcgctcgccc
      121 acatcgagga ggtcgagcgc ctcggcggca tggcgaaggc gatcgcggcg ggcatcccga
      181 agatgcgcat cgaggaagcg gcggcccgca cgcaggcgcg catcgacagt ggccggcaga
      241 ccatcgtcgg cgtcaacaag ttcaaggtcg aaggcgatgc cgcgatcgag gtgctgaggg
      301 tcgacaacaa ggccgtacgc gaagcgcagg tcgccaagct cgagcggctg cgcggggagc
      361 gcgatccggc agccgtgaaa gcggcactcg cagcattgac tgcagcggcg acgggcggcg
      421 gcaatctcct cgaagctgct gtgaacgccg cgcgccttcg cgcaaccgtc ggcgagatct
      481 cggatgcctg cgaggcggtc tacggccgac atcgcgccga aatccgcgct gtgagtggcg
      541 tctatcgatc ggagagcgga gccatgtcga aggagatcga gcgcgtccag gcgatggtga
      601 aggcgttcga aaccaacgat ggtcgccgcc cgcgtatcct catcgccaag atcggccagg
      661 acggccacga ccgcgggcag aaggtgatcg ccaccgcctt cgccgatctc ggcttcgacg
      721 tcgacatcgg cccgctcttc tcgacgccgg acgaggtggc acggcaggcc gtggagaacg
      781 acgtgcacgt gatcggcgtc tcctcgctcg ccgccggaca cctgacgctc gttcccgagc
      841 tcaaggccgc actcgacaag gaaggccgcg gcgacatcct gatcgtcgtc ggcggcgtca
      901 ttccgccggc ggactacgac gctctgctcg cggccggcgc gaaggccgtg ttcgggcccg
      961 ggaccaacat cccggcggca gcagccgacc tcatcgccaa gctcaatgcc gcacttggct
     1021 atcagccgaa ggccgccgcg gagtaaaggc ggccacggag aggagtaacc catgaacgcg
     1081 cgtccgagcc tcgccgtcga caaggccacc ttctggcgct tcgtcgagcg cgatccggag
     1141 ggacgatacg agtacagcag ggggctcatc gtgcagcaga tgccgggtgg gaccctccgc
     1201 cattcgttga tcgcaaagcg cattgccgcg ttggtcgagg ggcagctcga tcctacggcc
     1261 tgggtggtga tcgagaataa ccgggccgtc gagactgccg agagcatccg ctaccccgac
     1321 gtggtcgtcg agcgcctacc cgccgacctc gactcgcgtc agacgcttgt cccgctcatg
     1381 atcgtcgagg tgttgtcgcc gacatcgcgt gtcatcgacc tcggcgacaa ggtggacgag
     1441 tatctccaga tcgccgcgct tcaggtctac gtcgtggctg atcagtctct ggcgcgcgtc
     1501 agggtctggc tgcgcggcgc cgacggtcat ttcccgagcg agccgacgat catcgaaggc
     1561 ctcgagagcg cgctcaccgt ctcgagcctc ggcatcgtcg tgccgctcgc agccatctac
     1621 gacgggcttc tgacggggcg aggctgaggc gcgagcgccg aaacctgcaa tctttccagc
     1681 tatttgccgc cgacgatcac agccaggatc tccggcgcga agcgctcgac gagggcaatg
     1741 ttctccggca tgcggggctc tggctggccc tttccagctg cgatctcggc aagctcgtcc
     1801 tcgatcgctt gcgcggcggc cttcttggag tcggcgtcca tgtacggcga gccgcgaatg
     1861 tcggcgagct gcttcttcag tcgatcctgg cgcgaaacat atcgcttgga gccttggtga
     1921 tcgatcccgc cgaagatcga ggcgatggtc cagctgatgc tgtagccgtc cgcggtgtcg
     1981 aagccatgct tcttctgcac ggccttgagg ttcgccatga actcgtcgat ctgaccgggt
     2041 ttcatggcgg tgtagggccg ccgaacttcg atctcctctt tcgcgtgcgc catgtatctg
     2101 cgcacgtccg cctcggtgag acgcacttcc ttgggctcga gggcgagggc atcggtgctg
     2161 cctgcagctt tgtccttggc cgctgtgggc gtactctctc cgcagccgct cagggccaag
     2221 gcgaccgtcg cgacggcaag caaggcgagg taggtcagcg agcgcgtcat ggatcggttc
     2281 ccgtttatgg cagtatcgtc atacttcgca aatttgcgac gctcagagac aattcgcact
     2341 cgagttcgaa atcgatagag gcttacgatg cacaagcgac attcatggac ctgcgccgtc
     2401 gccgtggccc tgctgtggct ttcgggcgcg aacagcgcgc ttgcggccgg ttcctgtgca
     2461 ttggtctatc agagcccgga caacgggatt tgggtgttgg acaacgcagg gcgccagatc
     2521 ggctaccggc tgtcggcgaa gaccggcagc aagttcgagc tcagacgtgc ggtcaccatt
     2581 ctctacccca gcggccaact catggtgacc tgcccgaact gcccggccga gcgcgcaccg
     2641 atgatcgaga cgcttctgtt cgactcggcc gccaaggact tcaacgcgcc ggaaaagcac
     2701 ccgccggcct atcgcttcga gctcctcaac gacgaggcca aagccgccaa caccgtgacg
     2761 atttcggagc cgctcacggt cgagatcgga ccgctgaaag gctgggccgc cgccatcgag
     2821 ctcgttcaca agacgacggc cggccagcgc tcggccttcg tctacagcta tgtgcacgac
     2881 aactgcctcg ccggcttctt catgttcacg ttcgacatcg acgccaagag ccattggctg
     2941 ccgcagagcg aactcattgc cgagttcaag gctgcgctgg ccagcttctc gatcgagacg
     3001 ttcgatccgg ccagtgaccc cgcggtcgct gccgaactga agaagaagct cggcaagtag
     3061 tgctcgctcg tccgggtcac gccgcaatca gccagacggg ttgcgcgccg aggcccttca
     3121 cctcgatctc gccgcgggcc tcgagcttga agtgcgcctc gatcagctcg cgcgtgcgcg
     3181 ccgaaacctg gacgcggccg ggcacaccgt tgctctcgag gcgggcggcg aggttcaccg
     3241 tatcgcccca cacgtcgtag gtgagacgcc gcactccgat cacgcccgca agcagcggcc
     3301 ccgaggcgat gccgacacgc agctcgagcg gcttcccgac ctctgccgcc agccggccga
     3361 tcacgccatg catggcgagc ccgaagcggg cgaggcggag cgcgtgttcc ggctgcggtt
     3421 cgggaacgcc tgccgccgcc atgtaggcat cgccgatcgt cttgattttt tcgacgccgt
     3481 gggcggcggc aagcgcatcg agttcgcgga cgaggcggtt gagcagctcg accgtgcggg
     3541 ccggcccgag cgagagggcg accggtacga agccgcgcat gtcggcgaac agcaccgagg
     3601 cgtccgagaa gctgtccgcg atggtgacgc ccggattggc cttcagccgc tcgacgatcg
     3661 gctcggggag gatgttgcgc agaagcgcgt cggtctcggc ttcggcagcc tcggcgagac
     3721 ggaacgcgta gtgcacgatc gccgccgtga tcgcaaaggt cgtcacgatc gccgagacgt
     3781 agatcgagcg gatgaaggcc ggatgagcgg cgaactcggg ctggggcgcc gggaacagca
     3841 cccagacggc cagatgcatg acgaggcaga tcgcgaccag ggccagcgaa agccaccgtc
     3901 ggctgaggcc gaacacgaag aacggcgccg ccgcaccgat gatgtactgg atgtgcacgc
     3961 cggacgggct gccaagatag gccccgagca gtaacaggcc gaggagctct gccgctgaga
     4021 tgaggagggc tccggcgatc tcgccgaagc gatgcatgaa ggggacgagc gcggccgacg
     4081 ccgcgagcgc gaagttgatc cagatgacgg gcgcaaacgt gcggtagtcg acgaacaggt
     4141 gctggagcga gtagccgaac gtgaagatcg ctatgaggta ggcgaagaga ttgaggatca
     4201 ttagccgacg gcggacggca ggcggatagc cgtcggtgcc gtagcgtgcg agcgcggcga
     4261 cgcgcggcca ccaggcaccg agcgactcga gcgttggcag cgacaatcgc gtcggcgccc
     4321 acatcgcgtc tcgtctcccc acgtctccat tcgcccaggg cgccgagtgt cgcccatcca
     4381 cgccggatcg ggcaatggct tcaagcttcg agacgctgat cgcggcgctc gagcactgcg
     4441 acgacgacat aggccaggac tgccgcacca agccctgcaa gatacggcgt cttcagctcg
     4501 aagacgaagc gtccccagcc ccagatgacc gcgcctgcaa ggatcgcggc gagtgcactc
     4561 tgcggaccgc caaagcgcgt gaagaggccg aagatcagcg caacgaacac gccggcgctg
     4621 gcgaaggccg aggcgagctc gacgagctcc ttgatgctcg ttgcggcaag cgcgatgacg
     4681 taggccgtcg cgccgagaac tgcgacggtg acgcgcgtgg tcaccacctt gccccggtcg
     4741 gaggtgtccg gattgagtcg aagcaggata ttgtgcgaga cgagcgagcc ggcagcgagg
     4801 agcgctgaat cgaccgtcga caggatcgcc gagatcagtg cgccgatgaa gacggccgac
     4861 agaaccgcgg gcagataagc cttggcgagc tcggcgacga gctgctcgct ctgctcggcc
     4921 ggcagcgacg gcaaaagttt cgggccgagc aggccgaaga cgaccgggat cagcgcgacc
     4981 agcagataga gcacgccgcc cgagatggcc gccgtcgagg caacgccggc cgagcgcgcg
     5041 ccaaggattc gcgaaatgag ctcgacggcg acgagcgagc cgcagatcgg caccgcccac
     5101 tgctcgatga actcgagcca cggctgatcg gcaaccatga agctccagcg cgccggctcg
     5161 atggcggcga ggcccgcgga aaagccaccg gctttcgcga gcacgaagaa gaggagcatc
     5221 aggagcccga cgatgatgac gatgccctgg ataacgtcgg tccaggcatc ggcgagcagt
     5281 ccgccgacga cggtgtaggc gacgaccgtt acggcgccga tggtgatcgc cgtgcgcgta
     5341 tcaatattgg cgaccgaggc gacgatctgg ccgaagccat agatctgggc cgcggcccac
     5401 aagactgagc ccggcaccag cagcacggcg accaccacct cgacagtcgg cgagaagcga
     5461 ttgcggaaga agtcgccaaa cgttgtgatg ccggcgcgcc agagccgccc cgccacgaaa
     5521 aggcccatga ggatgatgcc gacggcatag gcgaagggct cgccctgcgc tccactcaag
     5581 ccgctcttga agacgctgcc ggcgcttccc gtgaccgttt cggcgccgaa ccaggtggcg
     5641 aagaccgaga agccagcgag tccgacgccg agcgagcggc cggcaagaat gtagtcggtt
     5701 tcgttcctaa tgctgcgtga gacgaggacg ccgatggcga actgcaccag gatgtagccg
     5761 atcaccagcc acaggatcag gttgcccatt gcacgcgctc caagccgacc gacagtatcg
     5821 atctgtgaac cagtgacgat ccgtgaacca gcattctgaa tgacgtccat accaatagcc
     5881 cagggccggc gacgcgggtc aatcttgaac gtgccagaac gataaattcg ccgacagggc
     5941 atgcaatcag gcctcgttca tcgtcgaaca gtgatgtatt gacggcgcac ataccggtga
     6001 cgaaggcttg acgtagtggt cctctggcca ttgcggctga aaaaggggtc gaaccttgca
     6061 gacgccgaaa agaccctcgc gagtagtggc attcggggtg tcgctggcac tcgcggcggc
     6121 gcacgtgtcg gcaccaggcg cggcgcgtgc ccagtcgccc gccggaatga cccgcgccga
     6181 ctacgaggcc gccnnnnnnn nnntacgagg cttgtcaggc gcgtgacgag gccgctttcc
     6241 gcacggcgat cgagggcgtg accgtgcgcg ggttgcgcaa aggcctggcc ggcctcgact
     6301 ttccggccgt cgtcctgcac gagtggcgtc gcaacggcat ggacgcgatc atggacggcg
     6361 aggtcgagaa ggtcgccgcc cagctgcgca gcgagagcgg ctggttcgac atgtggggca
     6421 cgctcttctc caaggacaag gcgacccgca tcgctacgga ggccgccgag cgcgtctacc
     6481 gctccgatgc tgtccgcaag ggcttcgaga cgctcgccaa cgccatcggc cgggaagtcg
     6541 gcgagcgcat ggagatcgcc tctgccgacg cagccgagcc ggcgacgcag tgcctcgtcg
     6601 ccttcgtcgg cccgcgctac ggctcgacga ttgcccgcat cgtcggtcgc gatgcgacca
     6661 aggacatgtc gggcgcttcc gacaagggaa aggccgccgt cagcacgggc tcgctgatga
     6721 tggaaggcgc cggcggcatt gccggcgcca tcgtgctgct ggtccgccgt caactttcga
     6781 acatgggcgc ccgcatcggc cagcgtgtcg tcggcgccgt gctcgggcgt gtgatcgccg
     6841 tggtcgcggg cggcgtcggg ctcgtgctga tcgccaagga cgtctgggat ttccggcatg
     6901 gcgtgatccc gatcgtcgcg aacgagatga cctcggccga gacgaaggag aaggtccgcg
     6961 tcgagattgc gcgggcgagc tacgagcaga tctccgagca catgcaggag atcggcagca
     7021 aggccgccga ccgcatcatc gagatctggc acgagttccg ccgcgcgcat gcgaaggtgc
     7081 tcgagatcgc ggagcgcagt ccggccttca aggcgtttct cgaatcgatc cgccccgacc
     7141 gcctctcgcg actcgacgag atggtcggca tcctcctcct ggccgaagga gaggccggtg
     7201 tcgtgaagcg cctcgagaac ggcacgctca accgcgcggt gaaccagctc gagccagcgg
     7261 ccttcgagat cgcgcgcgac acgaaatcgc tcgatgccgc cctgcaatgg gccgatctcg
     7321 cacccgagca gctcgcccgc gtggtcgaga acgagctcca ccgcaaagca gacccgaagg
     7381 agttcacgaa ggcggcgctc acgcgcatcc tcgccctgcc ggaccggctg gcgatcagcc
     7441 ggctcgcagg cctcaagacc aaggcccgcg acccgctgat ggagctcgac gaccagcgcc
     7501 ttgttggtct cgcgcgcgcc ttcgatacgg gcgagctcga tacgctctcg cgctacctca
     7561 ccggtctcga ccgtgaggcc ggccagcgcg tgctgatcgc cgtcgccaat acaccagcga
     7621 agatggcact tctttccaga ccgtccgtgc gtgacgccat cctctcgagc cgcaatcaat
     7681 ctgcggccat cggcatgatg ctgcgcgcgg acggcatctt cgatgtcggg atgttcgtcg
     7741 aggacctgag cctcgtccgc catggcctgg tcagtccatg gctgatctgg gagcgatatc
     7801 cgtatgcact cccaggcctc ggtgtcctcg gcctcgttct gctgctcgcc atctggcgcg
     7861 ccctcttcgg tcggcgccgg gcggcaacgg tcatcgtgca ggcgcccgcg ggctcctccg
     7921 acaagcgggg ggcggcatga ggatcgtatc gacggagcat gtcgaggatc gcctcatcga
     7981 gaagggcgag ttcgtttatg ccgtcgccat cagcggcgcg aatatcattc gcgacatgcg
     8041 cgagacgatc acgaacacga tcggcggcca catgacgcgc tatgaggacc tctgcaacca
     8101 gacgctcgat cgcgcgctgg caagcctcaa ggagaaggcg gcggcgacag gctatgacgg
     8161 cgtgctcggc gtgcgcatcg agcatccggc gataacgcag ggcgcggtgt cggtcgtggt
     8221 tttcggcacc ggcttccgct accgcgacta agtggccgag ccccgcggtc gtgtctggca
     8281 gaaccggccc cggtccctca tattgagtgc gagaaccacg ggcacattgg actgatgacc
     8341 cagcagacgt tcacccaatc tctcgccaac ccgacccgat tcatggcgct gtcggcggca
     8401 ctgctgcctt ggatcaccgg ggcggcgggg ctcgtgctcg ccgtcgggct cgtctgggct
     8461 ttcggcttca cccctccgga ctaccagcac aaagagacgg tgaagatcat gttcgtgcat
     8521 gtgccggccg cctggatcgg catgggcgcc tacacgctga ttgcggtctc cagcgtagga
     8581 ctcctcgtct ggcgccatcc gctcgccgac gtatccgcca aggccgcggc acccatcggg
     8641 gccgccttca cgctcgtctg cctcgtcacc gggtcgctct ggggcatgcc cggatggggc
     8701 acctactggg agtgggacgc gcggctgacg tccgtgctcc tcctcttctt cctctatctc
     8761 gggttgatcg cacttcgcac ctcgatcgag gacgagagcc tcgcctcgcg cctcaccgcc
     8821 attctcggca tcgtcggagt cgtactgctg ccgatcatca agttttccgt cgactggtgg
     8881 tggacactgc accagaaggc gagcgtgatg acgcttggcg agcagaaggt gcactggtcg
     8941 atccgggcac cgctcctggt gatggcgctg ggcttcctgc tcgcctttat cgccatgcac
     9001 ctgaaggcca tgcagaacga ggtgctgcgt cggcgcatca aggccatccg gatcgcgcgt
     9061 gtcgcggccg agacccggcg tggcgcgagc atcgatgcgg cctcgggccg gcaaccggcg
     9121 gagtaggcgc atggcgagcc tcggacagca cgaaagctac atcatcgcgt cctatgccgt
     9181 gaccttcatc atcctctgcg ccgtcgtcgc ttggctcgtg atcgacggcc gccggcagcg
     9241 cgaattgctc gccaggctcg aggcaagcgg cggccggcgc ggcgggagga cgtcagtcgg
     9301 cgacaagggc gcggcatgag cgtcggcggc agcgaaatgg gcgagcgcgg aactccggca
     9361 ccgcgccggc gcgtgccgat cgccgtcgtt ccggttgcga tcttcgcctt gatggccatg
     9421 ctgttcgcgt tcgcgctcgg caagggtgac tcgagccgcc tgccatcggc gctgatcggc
     9481 aaagcggcgc cggtgatgca gctcgccccc gtggacgggc tctcggatgg agtgcgcccg
     9541 gttcccggca ttgcaccggc agatctcgcc aagggtgacg tcgtcgttgt caatttctgg
     9601 gcgtcctggt gtccgccctg tatcgacgag cacccgctgc tcgtggaact cgcgagtgag
     9661 acgaaagtgc gcatgctcgg catcaaccac aaggacacgg ctgcaggcgc acggcgcttc
     9721 ctcggtcgct acggcaaccc gtttgcgggc gtcggcaccg acccggacgg tcgcttcgcc
     9781 atcgaatggg gcgtctacgg catgccggag acgttcatcg tcgatggcaa gggccgcgtc
     9841 gtctacaagc atgtcgggcc aatcacgcgg cagagcctgc gggagaagat catcccggcc
     9901 atcgaggtag cgcgtcgcgc tggaagttag cgcctctggt cctgacggag tgcctggcgc
     9961 tgcaggcgca tgccgatcat ggtcatccag atgccgagga tgccgacgag cacgttcagg
    10021 atgttgatga ccatctcgaa gttgccccag agcccgctca tgagcccgcc gcccgatccg
    10081 ggcgtgccgg tcgtcgtgcg gctggtcggg gggcgctccg gtatgtagga gcgcccgcgg
    10141 tcggcgggtg cgcccgcact cggggcggag agtgccgtgc gtgcccgttc ggtcgaatag
    10201 agcgagcgat aagcgaagtt tccgatcgcg ccaccaatga ggacgagccc caacaggacg
    10261 aaaagcatct tgcgcatctg tgcctcgact gatcgatcaa gcgaattctt cggttgtggg
    10321 tagcttaccc cacgcgcgcg ggccggtcat atcagaccgg acctcgctcg tcatcgccgg
    10381 caggtcaaga ctgccggaag gcggctgatt gatggctcga cgggcgttat cgacgccggc
    10441 cgtggaacgt gtcgagcagc cggcggtcgc gcttcgtcgg ccggcccatg ccgcgcggac
    10501 gctctggcgg cagcatgggc ggcgcttcgg atttctcgcg cggctcggcc tcttgcgccg
    10561 cgagcggctg gccggatgtg tcgagctcct ggtagagcgt ctgtgcctcg ctcgccggtc
    10621 cgcgacgcgc gccgagtgcg acgaccttga cgatgcgcac cgtcctttgg atcgaggccg
    10681 tgatcacgtc gcccggacgg atcgcctgcg ccggcttggt gatacgctcc ttgttgacgc
    10741 ggatcttccc ctcgctcacg agcgacgcag cgagcgtgcg cgacttgatg agtcgcgtgt
    10801 accagagcca cttgtcgaga cgctgcgtgc ccgccggcga gccatctgcc tcggcgtcgt
    10861 cgtcgccatc cttcgtcgac ctggccatgg cctcacgtca cctcatctgc tcgcgtgtcg
    10921 gtccgcggac gcaagcgact tcaacgcttc gagcatcttg acctcgaccc caaccgtgcc
    10981 cgccgctgca tgggcggcgc gcctttccaa ccgtcgcatg ccgggcagtc ggacaccagg
    11041 gtctgccagc atggcgccag tgatcgagga gaggcgctcg aggaacgtca cgcgggcacc
    11101 gaaggcatca ggatcgatcg ccacgagcag ctggccgaca cccggcgggg gaccgtcgcc
    11161 gtcgaagaac gagctcgccg cgtgggacgg attggcacca gtgagcgcca cggcgagcac
    11221 ctcgaccatc aaggcgagcg ccgcgccctt ggtgccgcct gaggggttca acgagccggc
    11281 aagtgcggcc ttggcatcgg tcgtcggacg cccttccgca tcgaacgccc agccttcggg
    11341 aatggcctcg ccccgctgtg ccgccgccat gatcctgccg cgcgcgacct cggacaaggc
    11401 gaggtcgacg acgagcggcg cgccctcctg ctgcggcgcc gcgatggcga tcgggttggt
    11461 tccgaagaca ggccggcgtc cgcccgccgc ggccatcgcg cccggcgtat tgccgaacgc
    11521 gagcgcgacg agtccttgct ccgcgagcct ctccacatgt cgtccgacca caccgaaatg
    11581 atgcgagcgc acgaaccccg ccgcggcaat cccggcctcg tgcaccatga cgggcagcgc
    11641 ctcgatggcg aggtcaaatg ccggataggc gaagccgccc gccacgtcca ccattgctga
    11701 tccggacctc gtgcgcgtga ggagcggcgt cgcgaagccg tcgaccttgc cgctccgcga
    11761 ttgcgccgcg tagctctgca cccgggatag gccgtgcccg ccctgaccgt cgatctcggc
    11821 ggcaacgagc gcgcgtgcaa ccgagcgcgc gttggcctcg ctcgtccggc tggcgacgag
    11881 cgcccgtgtc acgaggtcct cgagcgctgc ggccgtcatg cgctccgtcg ccatcagacg
    11941 agcgcctgca tgtcgccgtc gatgctgatc gcctggccgg agatggtgcg cccgcgcggg
    12001 ctcgcgaggt agacgatcat gtcggcgagc tgctcgggtg cgatcatcgt cttcagtgag
    12061 acgttggcga gggcggccgt cttgatctcc tgcatggact tgccggcgac ctgcgccttt
    12121 gcctcgaaga cgcggcggat gcggtcgcct tcgaccgttc ccggaaggat cgcgttgcag
    12181 cggatgccga agccgccgag ctccatggcg atcgtcttcg tgaagccgat cacgccccac
    12241 tttgcggcgg catagggcga gcggttcggg aatccgaatt tgccggccgc cgatgagaga
    12301 ttgatgatcg agccgttctt cgaggccttc agaggctcga ccgcgagccg cgtacagttg
    12361 aactggctgg tgatgcagac ctcgaggcag cggtcccagt cctccgggtt gatctcgtgg
    12421 actggccccg tcggaccggc aatgcccgca ttgttgacga gacagtccag cccgccgagc
    12481 ttcgcaagcg catccttgaa gagggccgcg acctcgccgc ggtttgcaac gtcgcacaca
    12541 ctctgcgtca ccgcagggtg cgacttggcg agcgtcgcca tcgccgccgt gtcgacatca
    12601 cagacgtgga ccctggcgcc ctccgacaaa aacgccttgg tgatttcgag gccgatgccg
    12661 ccggcgcctg ccgtcaccag aacacgcaat cccttgatgc cgagctccat tggcgctgct
    12721 cccattttgc tctgtgttcg cgagtgttcg cgccaaacta atgacgaccg cttgcattgt
    12781 caccatccgg ggtcacacgc ggggtcaggg cgtgacctcg ggtgttgtgc cttaggcaag
    12841 cgccgagctc agcgttgcaa tgccccgggc gaaaccttgg tgcgggacgt cgcaagggct
    12901 caccccaccc aacatctccg cctcacacgc atcgcccacg atctgttatg gtcgggcttg
    12961 ccccgaccag ccatggggcc gcgccatcag gtgtccgccg atggatgact cctcgggaca
    13021 agcacgagga tgacactcgc ggaggctgca tcgccactac cgcccagcca cctatatcgt
    13081 catcccgccg gaaggcgaga cccggggcac gtcggagtgc ccgccatagt tcccccgcat
    13141 ggtgacctgg gtcgtcgccc tcgcgaggat gacgagagtg ggacgagagt cgcgtgcctg
    13201 ccgaagtcgt gctcttcgct gacaatcctg ttgatccatc cacgcgcact gcatcgaccg
    13261 ccgcgcgaat tcccttgcct cgctcgcggc gccatcgcag tttttcaggc cgtcgccagg
    13321 aggagagcca catgacacgc cgcaagaagc cggaggagct gcgctcgcat cgttggttcg
    13381 gtgtgtccga cctccgctcg ttcgggcacc gctcgcggct ccgtcagatg ggcttcgacg
    13441 cggacgactg gcagggccgg ccggtgatcg gcatcatcaa cacctggagc gacatgaatg
    13501 cctgccatgc gcacctgcgt gcgcgcgtcg aggacgtgaa gcgcggcgtg ctgcaggccg
    13561 gcggcttccc ggtcgagctg ccggccatct cgctcgccga gccgatgg
//
LOCUS       DAORNH010000102        13601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3420_length_13601_cov_2.689355, whole genome shotgun sequence.
ACCESSION   DAORNH010000102 DAORNH010000000
VERSION     DAORNH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3420_length_13601_cov_2.689355"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>163
                     /locus_tag="PK264_11815"
     CDS             <1..>163
                     /locus_tag="PK264_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD76609.1"
                     /translation="LAANQLIQKKLADMQTEIAIGLQGVLRLGRMMDEGSAAPELVSL
                     MKRNNCGKAL"
     assembly_gap    164..328
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    563..581
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <582..1615
                     /locus_tag="PK264_11820"
     CDS             <582..1615
                     /locus_tag="PK264_11820"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD76610.1"
                     /translation="ERLRALGAPGVIIFVRRGSERWMATTGVADTRTKRSMTGKEVWR
                     IASVTKLITAVAVQMLAAEKRLTLTDPVSKFLPRIVPLASRITIKHLLSQTSGIPDYL
                     AGDNVPVNVSAAALARNLVRHRPRAALLADANRQKRRFAPGARHEYSNTNFLLLEMII
                     EKVTRQSFQKLLTAELIKPIGLTRTGFPDRDGNLPTPHIRGYVPLDTRKGPFTHSSRT
                     LDVTVHDYFSGADGGLYSNIDDLGKMMDALWSGAIIAPMQLRAMTTDLKSDHEGMYRY
                     GLGIVALPTRCGRKAFGHEGRDIGIFTTMLATEDGSRQLIVVVNTAIDYHPQMEKVLA
                     DLRDRVFCD"
     gene            complement(1629..3425)
                     /gene="ggt"
                     /locus_tag="PK264_11825"
     CDS             complement(1629..3425)
                     /gene="ggt"
                     /locus_tag="PK264_11825"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD76611.1"
                     /translation="MLNTSKGPAARVLLRPLRAFAALGLIAALVGGSALAQRAAAPGD
                     PEGATGVTDRRLAVAKRHMVAAANPLAASAGREMLRAGGSAVDAAIATQLVLNLVEPQ
                     SSGLGGGAFIVVWDVKSKQLRSYDGRETAPAATTANRFVVNGGVLPFDTAVGSGLSVG
                     VPGLARVMELAHQKHGRLPWAKLFEPALKLADNGFQVSPRLSRLLNSQGAQSFAPAAR
                     AHYFDEGGRARPPGYLLKSPEFAATLRALAGGGADAFYRGPIAEAIVRAVDQAPFAKG
                     RMTLADIAAYKARERSAVCFPYRTYKVCGMAPPSSGGLTVGMVLRLIEPLEIGKGRGA
                     AMSGAALHLIAEAERLAYADRDRYMADPDHVPVPRGLMDDAYLAERRKLIDPARAAAK
                     VEAGTPPGVGTNVFGKDATRENPGTSHLSIVDKDGNVVAMTTTIEAGFGSRLWAAGFL
                     LNNELTDFSMRPIDKDGKPAANAPGPGKRPRSSMAPTIVLDASGKPVIATGSPGGSRI
                     ILYVLKSLVGIIDWELDPQAAAALPNFGSRGESFEMEARFMAHPAIAALKAKGHRIST
                     DAMTSGTHTIVIRPGRLEGGADPRREGVALGD"
     gene            complement(3460..4062)
                     /gene="leuD"
                     /locus_tag="PK264_11830"
     CDS             complement(3460..4062)
                     /gene="leuD"
                     /locus_tag="PK264_11830"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRD76612.1"
                     /translation="MKPLTHLSAVAMPLDQPNIDTDQIIPARFLGRERDEQVEGLFRD
                     LRFTPEGKPRPEFVLNDPAYREAQIIVADRNFACGSSRENAVTALLDNGFKVFIAPSF
                     GDIFANNCFQNGCLAIVLPEERCRKMREHLRRNPGARITVDIEAQTVTGPDNEFARFE
                     IDSFRKDGLMKGLDDISLTLAYGDDIARFEARQKCDAGWL"
     gene            complement(4135..>4883)
                     /locus_tag="PK264_11835"
     CDS             complement(4135..>4883)
                     /locus_tag="PK264_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76613.1"
                     /translation="ECIIASAIALVICGIPASFQQHAYQPISKDGVAGATVDWARVFI
                     VFFILIAAIVANVTANLKAKWLLDTLPVLGFAVVAAILLTAPLRRPDWHLLGENLKGT
                     LFLLALVLCASLMPVEKLPPASWQSAMGLGFLSAVFDNIPLTALALKQGGYDWGFLAF
                     AVGFGGSMIWFGSSAGVAITNLFPEGKSVVRWLMAGWHVALAYVVAYLVMLMLLGWKP
                     DPPHRQRVEAPHPAAVVALWSRQPARDCLA"
     assembly_gap    4884..4966
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4967..5573)
                     /locus_tag="PK264_11840"
     CDS             complement(<4967..5573)
                     /locus_tag="PK264_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76614.1"
                     /translation="MRDVLSGRAGTGSAGVGHAGTLVARGATKLLLAAIAISIPTVAL
                     AASGSGGDPMLIGPVPVDFILFGLILLGIALFHHHTFYVAVAGLAAIVAYKLGFTGFK
                     TGAGLGGLVEHLKHEWITLANLFCLLMGFALLAKHFEDSRLPEVMPRFMPDDWKGAFM
                     LLAIVWFMSSFLDNIAAALIGGTMAKQVFRNKVHIGFIAAIV"
     gene            complement(5883..7361)
                     /locus_tag="PK264_11845"
     CDS             complement(5883..7361)
                     /locus_tag="PK264_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76615.1"
                     /translation="MKRGEIEEHGCDMVVVGSGIAGLSAAVTARHRGASVCILERAPP
                     EDRGGNTRWTEAFLRMKSEHAVAEDFISHFAKHAGHHLDPELVAETAGDFASRSAIAR
                     TLGFSDPDLIARFADEAPAAVQWLKGFGVRFGFLPMYFITTSTTRLGTVGGGRAMIEA
                     LGAWADANGIATHYRTTARALMQDETGAVVGVEAVADRNRPVRFRAPAVVLACGGFEG
                     NPEMLARYLGPQSRYIRPVARGGYYNRGEGLAMALAIGAAPSGDWARFHAEPLDPRSG
                     APEPVVMVFNYGILVDTRGRRFTDEAPSSADATYEAVTRIIGEQPEGMAWLILDARID
                     RIAGWQRAVRSDQPAIEAPSLADLALRIGVPPDELAATVKAFNAAVVSPEAAFDPERP
                     DGVRTRAGYEPRKSNWAFPLAVAPWRAYPIICGNCFTFGGLKVDPDARVLNMDGEAIP
                     GLYAAGETIGLYWGTYTGATSVLRGAVFGRIAGAAASPGATT"
     gene            complement(7488..8717)
                     /locus_tag="PK264_11850"
     CDS             complement(7488..8717)
                     /locus_tag="PK264_11850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD76616.1"
                     /translation="MDRPALRFAEFVLDLDAAELRKNGTPVRMEPQVFDLIAFLASHP
                     GRVLSKEDIVNGVWHGRAISDSAISTRINAARRALGDDGTAQRFIKTVHGRGFRFEVG
                     TRVLPSSEPAEHEGAEQLGPVEVRYCRTRDGINVAYGETGNGAPLVKAASFVTHIQYD
                     SEGILFRDWIRSFTRFSRYIRYDERGNGLSDWSIDDFSFDKMVTDLEAVVDELALGKF
                     VLLGCSQGASVSIAYANRHPERVAGLIIFGGYAAGWRRSSDMKHRERREALLQLARVC
                     WGDDNPTARQAYAALFYPDGTPEQHAEFMKLQRDTVTPENAFRIMSTFAEIDVRGLLP
                     SIKVPTLVMHCKDDAVVPVSAGKEIASGIKGARFVLLEGRNHILQASDPGWQRFTSAI
                     REFVHRHFGRGVRANEP"
     assembly_gap    8957..8971
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8972..10258
                     /locus_tag="PK264_11855"
     CDS             <8972..10258
                     /locus_tag="PK264_11855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD76617.1"
                     /translation="YNGMIDKRPLLIAKCADVADVMTAVNLGRDNGLRIAIRCGGHNG
                     PGLGSVTDGLVIDMSAINGVRVDPAARTVRVGAGCTQGDVDHATHAFGLAVPAGIIST
                     TGIAGLTLSGGHGYLTRKFGLTIDNLIEADVVLADGSFVTASQDKNADLLWALRGGGG
                     NFGVVTSFLFRAHPVKNVFAGPIAFDQKHAPTIMRKFHNFLGRAPEDLGIFVGLKTVL
                     SCDPFPKEHWGKRICLLMCCFNGSEADGKSALAPLLGELPEPWFNWTGMMPYPAVQAM
                     FDGLYPKGMQWYWRGDFVKSLPDAAIDAHLAQAAKAPSELSLMHLYPIDGAVHRANKG
                     DTAWNCRDATYSMVIAGIDPDPKKAAAVTQWTKAYWDAVHPFNLGGAYPNFMMDDEGD
                     ARIRASFGDNYARLAATKAKYDPNNLFRVNQNIKPA"
     gene            complement(10702..10791)
                     /locus_tag="PK264_11860"
     tRNA            complement(10702..10791)
                     /locus_tag="PK264_11860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10755..10757),aa:Ser,seq:tga)
     gene            complement(10831..10968)
                     /locus_tag="PK264_11865"
     CDS             complement(10831..10968)
                     /locus_tag="PK264_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76618.1"
                     /translation="MMPRGVRRLVMLALGTLIVGALVLVAVRGEAMLLDLTAAIAAFC
                     L"
     gene            complement(11111..12340)
                     /locus_tag="PK264_11870"
     CDS             complement(11111..12340)
                     /locus_tag="PK264_11870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76619.1"
                     /translation="MSAQSARPLWLVILSAALVVGVAMGLRNVMGLYQVPLVSEMASK
                     FDPTIGRETFSLAMAIANIVWGIGSPFAGAISDKFGTGRVIVGGALSTMGGLYLMYIA
                     TTKLELMLSGFLLGIGVSGTGVTALVGAVGRMAPPEKRMSAMSAVGMGSGLGILIALP
                     YTHFLIENLGWRGSLLVVLFTTAIIIPFAFPLAGKPQQLGGEKPQTLGEALSEAFAHP
                     SFWLLIAGFFVCGFHVTFVGVHLPAFAKDQQLPAWVGSWALMGVGAANVLGTWLAGQS
                     AKIVEKRISLSLIYFARAIIFLGFIYLPMTEVTVIALAAALGLFWLSTIPLTSGLVAT
                     FFGPAWMSMLYGIVFLSHQIGSFLGVWLAGYLYDLTKSYTAMWWISVGLGLFAAIVHW
                     PIKERPVERSPVLATAA"
     gene            12548..13132
                     /locus_tag="PK264_11875"
     CDS             12548..13132
                     /locus_tag="PK264_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HRD76620.1"
                     /translation="MDIGATPASENEFRTRAISIYIGIAAVLLAICTMAGGNATKDAM
                     RANIEAANTWSFFQARVIRRSLLELSSEDLALMQKSQPGLPETAQKLIAERIARNQSI
                     AKAYTSNPERRDGADELFKKARELEAERDLALRRDPYFDYAQAMLQISIVLASVAIIA
                     GGSALIQVSIGLGMIGTLLMLNGFTLLTRIPGIG"
     gene            complement(13179..13598)
                     /locus_tag="PK264_11880"
     CDS             complement(13179..13598)
                     /locus_tag="PK264_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRD76621.1"
                     /translation="MLDVADQITALGFQAWSPQSFVEGISSFYLVLQVAFGSMGAIAL
                     LVAAIGIANTMTMAILERRGVIHRHSFEGAPARYETADDKHHDHIIDIDTGAVIEFRS
                     AKIEELQAKIAAELGFEVVHHRLELYCRKARPKPDEG"
BASE COUNT         2459 a         4400 c         4320 g         2140 t
ORIGIN      
        1 gctcgccgcc aaccagctca tccagaagaa gctcgccgac atgcagaccg agatcgccat
       61 cggtctccag ggcgtactcc gcctcggccg catgatggac gagggcagcg ccgcgcccga
      121 actcgtctcg ctcatgaagc gcaacaattg cggcaaggcg ctcnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aggcgttttt ctgagccgta acctcgttct
      361 gagcttgtgg ggagggggca gtcacggatg ctgctaccgt gcgaccgcgc tccgacgacc
      421 tgccacgcaa cgccgccccg ctgctaatcc gatcgagaac cgcattgatg ccgctgctca
      481 aaattatgct caccagaaca ctctcggcgc tcgttgccgt ggctctcatc ctcgcgaccg
      541 tcgagaggcc ggcccgtgct acnnnnnnnn nnnnnnnnnn nccgagcggc tccgcgcact
      601 cggcgcaccg ggcgtcatca tattcgtgcg ccgcggctcg gagcgctgga tggccaccac
      661 cggcgttgcc gatacacgca ccaagcggtc gatgaccggc aaagaggtct ggcggattgc
      721 gagcgtcacc aagctcatta ccgcggtcgc cgttcagatg ctcgccgccg agaagcgtct
      781 gacgctcacg gaccccgtct cgaaattcct gccgcgcatc gtgccgctcg cgagccgcat
      841 cacgatcaag cacctcctca gccaaacctc cggcatccct gactatctcg ccggcgacaa
      901 cgttcctgtg aacgtgtcgg cggcggcgct cgcccgcaat ctcgtccgcc accgcccgcg
      961 ggcagcgctc ctcgccgatg cgaaccgtca gaagcgccgc ttcgctccgg gcgcccggca
     1021 cgaatactcg aacacgaact tcctcttgct cgagatgatc atcgagaagg tgacgcggca
     1081 gagctttcag aagctgctca cggcagaact catcaaaccg atcggcctaa ctcgcacggg
     1141 ctttcccgat cgcgatggca acctgccgac cccgcacatc cgcggctacg tccctcttga
     1201 tacgagaaag ggcccgttca cgcactcgag ccgcacactc gatgttaccg tgcacgacta
     1261 tttctcgggc gccgatggcg gcctctactc gaacatcgac gacctcggca agatgatgga
     1321 tgcgctctgg tcaggggcga tcatcgcgcc catgcaactg cgtgccatga cgaccgacct
     1381 gaagtcggat cacgagggca tgtaccgcta tggccttggc atcgtcgcgc tgccgacgcg
     1441 ctgcgggcgc aaggccttcg gccacgaggg tcgcgacatc ggcatcttta cgaccatgct
     1501 cgcgactgag gacggctcgc gccagctgat cgtcgtcgtc aacacggcaa tcgactacca
     1561 tccccagatg gagaaggtcc ttgccgacct ccgcgatcgc gtcttctgcg attgaggact
     1621 cggccgcctc aatccccgag ggccacgcct tcgcggcgcg ggtcggcgcc gccctcgagg
     1681 cggcccggcc tgatgacgat cgtgtgtgtg cccgaggtca tcgcgtcggt cgagatgcga
     1741 tgacccttgg ccttcagggc cgcaatcgcc gggtgcgcca tgaacctcgc ctccatctcg
     1801 aagctctcgc cgcgcgagcc gaagttcggc agtgccgccg cagcctgcgg gtcgagctcc
     1861 caatcgatga tgccaacgag cgacttcaaa acgtagagga tgatgcggct gccgcccggc
     1921 gagcccgtgg cgatgacggg cttgccgctc gcgtcgagca cgatcgtcgg cgccatggac
     1981 gagcgcggcc gcttgccggg tcccggcgcg ttggccgcgg gcttgccgtc cttgtcgatc
     2041 ggacgcatcg agaagtcggt gagttcattg ttgagcagga agcctgcagc ccacagcctc
     2101 gagccgaagc ccgcctcgat cgtcgtcgtc atcgcgacca cattgccgtc cttgtcgacg
     2161 atcgacaggt ggctcgtgcc gggattctcg cgcgtcgcgt ccttgccgaa aacgttggtg
     2221 ccgacgcctg gcggcgttcc cgcctcgacc ttggcggcgg cgcgcgcagg gtcgatgagt
     2281 ttgcgacgct cggcaagata cgcgtcgtcc atcaggccgc gcggcacggg cacatgatcg
     2341 ggatcggcca tgtagcggtc acggtcggca taggcgaggc gctcggcctc cgcgatcaga
     2401 tgcaatgccg ccccgctcat agcggcaccc cggcccttac cgatctcgag cggttcgatc
     2461 agacgcagca ccatgccgac ggtgagtccg cctgacgacg gtggagccat gccgcacacc
     2521 ttgtaggtgc gataggggaa gcagactgcc gagcgctcgc gcgccttgta agcggcaatg
     2581 tcggcaagcg tcatgcggcc cttggcgaag ggcgcctgat ccacggcgcg cacgatcgcc
     2641 tcggcgatcg gaccgcggta gaaggcatcg gcaccgccac cagcgagcgc gcgcagtgtg
     2701 gccgcgaact cggggctctt cagaaggtag ccaggcggac gagcacgtcc gccctcgtcg
     2761 aaatagtgcg cgcgcgcggc cggcgcgaac gactgggcgc cctgagaatt gagaaggcgg
     2821 gagaggcgcg gcgacacctg aaaaccgttg tccgcgagct tcagtgccgg ctcgaagagc
     2881 tttgcccagg gcaaccgccc gtgcttttgg tgcgccagct ccatgacgcg ggcgagcccc
     2941 ggcacgccga cgctgagccc gctgccgacc gcggtatcga acggcagtac gccgccattg
     3001 acgacgaacc ggttcgccgt tgtcgctgcc ggtgccgtct cgcggccgtc gtaggacctg
     3061 agctgcttcg acttcacgtc ccagacgacg ataaaggcgc cgccgccgag cccggaagac
     3121 tgcggctcga cgaggttgag gacgagctgc gtcgcaatcg cggcatcgac ggcggagccg
     3181 cccgcgcgca gcatctcgcg ccccgcgctt gcagcaagcg ggttggccgc cgccaccatg
     3241 tggcgcttgg cgaccgcaag ccggcgatcg gtgacgcctg tcgcaccctc cggatcgccc
     3301 ggtgcagcag cgcgctgcgc gagtgccgat ccgccaacca gcgcggcgat cagaccgagg
     3361 gccgcaaatg ctcgcagcgg acgcaacagg acacgcgctg cgggaccctt cgacgtgttg
     3421 agcatcccgg tccttcctgt cattgcgacc ccgaagtcat cagagccagc cggcatcgca
     3481 cttctggcgc gcctcgaagc gggcgatgtc gtcaccgtag gcgagcgtca gcgaaatgtc
     3541 gtcgaggccc ttcatgagac cgtccttgcg gaagctgtcg atctcgaagc gcgcgaactc
     3601 gttgtcgggg cccgtcacgg tctgtgcttc gatatcgacc gtgatgcgcg cgcccggatt
     3661 gcggcggaga tgctcgcgca tcttccggca gcgctcctcg ggcagcacga tcgcgaggca
     3721 accgttctgg aagcagttgt tggcgaaaat atcgccgaag ctcggcgcga tgaacacctt
     3781 gaagccattg tcgagtagcg ccgtcacagc gttctctcgg ctcgagccgc aggcgaagtt
     3841 gcggtcagcg acgatgatct gggcctctcg gtaggccgga tcattgagaa cgaactcggg
     3901 gcgcggcttc ccctcgggcg tgaagcgcag atcacggaag aggccctcga cctgctcgtc
     3961 ccgctcgcgg ccgagaaaac gcgcggggat gatctggtcg gtgtcaatgt tcggctggtc
     4021 gagtggcatg gcgacagccg agaggtgcgt gagtggcttc atcgagtgct ccattcgccg
     4081 cgccccgacg ttgcgcggca catcgaacga gtccggcgcc cgaacgccgg gcgatcaagc
     4141 gaggcaatcc cgcgccggct gtcgcgacca gagtgccacg acggccgccg ggtgtggcgc
     4201 ctcgacccgc tgcctatgcg gcggatcggg cttccaccca agcagcatca gcatcaccag
     4261 ataggctacg acgtaggcca gcgccacgtg ccaaccggcc atgagccagc gtacgaccga
     4321 cttgccctcg gggaacaggt tggttatggc aacgcccgcc gacgagccga accagatcat
     4381 cgagccgccg aagccgacgg caaaggcgag gaagccccaa tcgtagccgc cttgcttcaa
     4441 ggcgagcgcc gtcaacggga tattgtcgaa cacggcggag aggaagccga gccccatggc
     4501 cgactgccat gatgctggcg gcagcttctc gaccggcatc agcgacgcgc agaggacgag
     4561 cgccagcagg aacagggtgc ctttgaggtt ttcgccgagc aggtgccagt ccggccgccg
     4621 cagcggcgcg gtgagcagga tcgccgcgac caccgcaaat ccgagtaccg ggagcgtatc
     4681 caacagccac ttggccttga ggttcgcggt aacgttggcg acgatggcgg cgatgaggat
     4741 gaagaagacg atgaacaccc tcgcccaatc gaccgtggca ccagcgacgc catccttcga
     4801 gatgggctga taggcatgct gctggaacga ggcgggaatg ccgcagataa ccagcgcgat
     4861 cgcggaggcg atgatacact cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgac gatggccgcg
     4981 atgaagccga tgtgcacctt gttgcggaac acctgcttcg ccatggtgcc gccgataagg
     5041 gccgctgcga tattgtcgag gaagctcgac atgaaccaca cgatggcgag cagcatgaag
     5101 gcgcccttcc agtcgtccgg catgaacctc ggcatcacct cgggcagcct gctgtcctcg
     5161 aaatgcttgg cgagcagcgc gaagcccatc agcaggcaga acagattggc gagcgtgatc
     5221 cactcgtgct tcaggtgctc gactaagccg ccaagcccgg caccggtctt gaacccggtg
     5281 aagccgagct tgtaggcgac gatcgccgcg aggcccgcga ccgccacata gaacgtgtgg
     5341 tgatggaaga gcgcgatacc gagcaggatg aggccgaaca ggatgaagtc gacgggaacc
     5401 gggccgatca gcatgggatc gccgcccgac ccgcttgcgg cgagggccac agtcgggatc
     5461 gaaatagcga tggctgcgag caggagcttc gttgcgccac gcgccaccag agtgccggcg
     5521 tggccgacgc ctgcgctgcc ggtgccggcg cgcccgctca atacatctcg cattcgtccc
     5581 cccgtttccc agtccacatg ccgccggcca cgaataccga tcgctcgcct tcggcgtcga
     5641 tcgcggcaca gcaggtctgc tgcgcccggg cagcgatgac tgacactgca gtctctgcgc
     5701 tcgccccggc cttgtgccaa atcgtatcgg ggcaggactt gatcggcaac agtggatcgc
     5761 agacacaacg gacgcaggca cgcacgctca acgctcgggc atggcctctg caacgatcgg
     5821 ttcggctcga gttggggcga tgctacagcg tcgagcggcc ggctactcct cgccgcgctg
     5881 gcctacgtgg tcgctccggg gctcgccgcg gcgccggcaa tgcgaccgaa cacggcgccc
     5941 cgcagaacgc tcgttgcgcc cgtataggtg ccccaataga gaccgatcgt ctcgccggcc
     6001 gcgtagaggc cgggaatggc ctcgccatcc atgttgagca cgcgggcatc ggggtcgacc
     6061 ttgaggccgc cgaacgtgaa gcaattgccg cagatgatcg gataggcccg ccacggtgcc
     6121 acggcgagtg ggaatgccca attcgatttg cgcggctcat agccggccct cgtgcggaca
     6181 ccgtcgggcc gctctggatc gaatgccgct tcgggggaca cgactgctgc attgaaggcc
     6241 ttgactgttg ccgcaagctc gtcgggcgga acgccgatcc ggagggcgag atcggcaagc
     6301 gacggcgcct cgatcgcggg ctggtccgag cgcacggccc gctgccatcc cgcgatccgg
     6361 tcgatgcgcg cgtccaggat cagccaagcc atgccctcgg gctgctcgcc gatgatgcgc
     6421 gtcaccgcct cgtaggtcgc atctgccgat gatggcgcct catccgtgaa gcgccggccg
     6481 cgcgtatcga cgagaatacc gtagttgaat accatcacga caggctcggg cgcaccggag
     6541 cgcgggtcga gcggctcggc gtggaagcgt gcccagtcgc cggacggcgc cgcgccgatg
     6601 gcgagcgcca tggcaaggcc ctcgccgcga ttgtagtagc cgccgcgggc gacgggccgt
     6661 atgtagcgcg actggggtcc gaggtagcgc gcgagcatct cggggttgcc ttcgaatccg
     6721 ccgcaggcga gcacgacggc cggcgcgcgg aagcggacag gcctgttgcg atcggcgaca
     6781 gcttcgacac cgacgacggc gccggtctcg tcctgcatca gggctcgggc cgtcgtgcgg
     6841 tagtgtgtag cgatcccgtt ggcgtccgcc caggcaccga gcgcctcgat catcgcacgg
     6901 ccgccgccga ccgtcccgag gcgtgtcgtc gacgtcgtga tgaaatacat cggtaggaat
     6961 ccgaaacgga cgccgaaccc cttcagccac tgcacggcgg cgggcgcctc gtcggcaaag
     7021 cgcgcgatca gatcggggtc gctgaagccg agtgtgcggg caatggccga gcggctcgcg
     7081 aagtcgcccg ccgtctcggc gacgagctcg ggatcgagat ggtggccggc atgcttcgcg
     7141 aagtggctga tgaagtcctc agcgaccgcg tgctccgact tcatgcgcag gaatgcctcg
     7201 gtccagcgcg tgttgccgcc gcggtcttcc ggcggggccc gctcgaggat gcacacactg
     7261 gctccacgat gacgcgccgt gaccgcggcc gagaggccgg cgatcccgct gccgaccacg
     7321 accatgtcgc agccgtgctc ctcgatctca ccacgcttca tgaccggacc tttcgaggtg
     7381 acgcgcggcg cggctctcac acccgcgacc ggagcgtatc ccgtacagcg gagcgagcgc
     7441 tacgcccgcc cggctgtccc caatgtcaac tgtcgacacg aaagacttca tggctcgttg
     7501 gcccggactc cacgaccaaa atggcggtgg acgaattctc tgatcgcact cgtaaatcgc
     7561 tgccagcctg gatccgaggc ctgcaagatg tggttgcggc cttcgagcag gacgaaacgt
     7621 gcgcccttga tcccggatgc gatctccttg ccggccgaca cgggaacgac cgcgtcatcc
     7681 ttgcagtgca tgaccagtgt cggcaccttg atggagggca gtaggccgcg aacgtcgatc
     7741 tcggcgaatg tactcatgat ccggaacgcg ttctccggag taaccgtatc cctctgcagt
     7801 ttcatgaatt cggcgtgctg ctccggcgtc ccatccggat agaacagcgc ggcataggct
     7861 tgccgcgcgg tcggattgtc atctccccag cagacacgcg ccaactgcaa caaggcctcg
     7921 cgtcgttcgc gatgcttcat atcggaagag cggcgccaac cggccgcgta cccgccaaaa
     7981 ataatcaggc ctgcgacacg ttcgggatgc ctgttggcgt aggcgatgga tactgaagcg
     8041 ccctgcgagc agccaagcag gacgaatttc ccgagtgcca gctcatcgac caccgcctcg
     8101 aggtcggtca ccatcttgtc gaaggaaaaa tcatcgatcg accagtctga caggccgttg
     8161 ccgcgctcgt cgtaacgaat gtaacggctg aacctcgtga atgacctgat ccaatcccgg
     8221 aagaggatac cttcggaatc gtactggata tgggtcacga aactcgcggc cttgacgagc
     8281 ggagcgccgt tccctgtctc gccataggca acgttgatcc catcgcgcgt ccgacagtag
     8341 cgaacttcga ccggcccaag ctgttcggcg ccttcgtgct cggctggttc cgacgagggc
     8401 agaactcgcg tgccgacctc gaaccgaaac cctcgaccgt gcaccgtctt gatgaagcgc
     8461 tgagcggtgc cgtcatcacc gagcgcgcgc cgtgccgcat tgatccgcgt cgagatggcc
     8521 gaatccgaga tcgcgcggcc atgccagacg ccgttgacga tgtcctcctt ggagaggacg
     8581 cggccagggt gggaggccag aaaggcgatc aaatcgaaga cttgcggctc catcctgacg
     8641 ggcgtaccgt tcttccgcag ctcggctgca tccaggtcga ggacgaattc cgcgaacctc
     8701 aaggctggtc ggtccatcgc tcagtatccc tggtcgcgtt aggacgcctt cggacgaagt
     8761 atggcataga aaaatagcat cgatctggac aatttctgaa gacggcattc aagacgggac
     8821 ctggacgaaa acgccagtct cgggggggca ccaccacact gtgaggtact ccaatgcacg
     8881 ccaacgccat tgctatactg aagcagtcca tgcgcggcac ggtcatcacg cgcgacgacg
     8941 cccgatacga tgaggcnnnn nnnnnnnnnn ntacaacggt atgatcgaca agcggccgtt
     9001 gctcatcgcc aagtgcgccg acgtcgccga cgtcatgacg gccgtcaacc tcggacgtga
     9061 caacgggctg cgcatcgcca tcaggtgcgg cgggcataac ggtccggggc tcggcagcgt
     9121 gaccgacggc ctcgtcatcg acatgtcggc gatcaacggc gttcgcgtcg atccggcggc
     9181 tcgcaccgtg cgggtcggcg cgggctgcac gcaaggcgac gtcgaccacg cgacgcacgc
     9241 cttcgggctc gcagtgccgg ccggaatcat ctcgacgaca ggcatcgccg gcctcacgct
     9301 cagcggcggc cacggctacc tcacgcgcaa attcggcctc accatcgaca acctgatcga
     9361 ggccgatgtc gtgctggccg atggcagctt cgtcacggcg agccaagaca agaatgcgga
     9421 ccttctctgg gccctgcgcg gcggcggcgg caatttcggc gtggtcacga gcttcctgtt
     9481 ccgcgcacat ccggtcaaga acgtgttcgc gggcccgatc gccttcgatc agaagcacgc
     9541 acccacgatc atgcggaagt tccacaactt cctcggacgc gcaccggagg acctcggcat
     9601 cttcgtcggg ctgaagaccg tgctctcttg cgatccattc ccgaaggagc attggggcaa
     9661 gcgaatctgc ctcctgatgt gctgcttcaa cggctcggaa gcggacggca aatcggcgct
     9721 ggcgccgctg ctcggagagc tgcccgagcc ttggttcaac tggacgggca tgatgcccta
     9781 tcccgccgtg caggccatgt tcgacggcct ctatccgaag ggcatgcagt ggtactggcg
     9841 cggcgatttc gtgaagtcgc tgcccgacgc cgccatcgac gcgcatctcg cacaggcggc
     9901 caaggccccg tcggagctgt cgctgatgca tctctatccg atcgacggcg cggtgcatcg
     9961 cgcgaacaag ggcgacacgg cctggaactg ccgcgacgcc acctactcga tggtgatcgc
    10021 cgggatcgat ccggatccga agaaggcggc ggccgtcacg caatggacca aggcctactg
    10081 ggacgcggtg catcccttca acctcggcgg cgcctacccg aacttcatga tggacgacga
    10141 gggagacgcg cggatcaggg cgagtttcgg cgacaactac gcgcgcctcg cggccacgaa
    10201 ggcgaagtac gacccgaaca acctgttcag ggtcaaccag aacatcaaac cggcgtaggt
    10261 cttgctccca gaatgtctcg tgcacgatcc ggggcaccgc aggcgccaag cctgcaccat
    10321 caccgggcga gcatcatcgc gcacgagcaa ctagcccgaa ctatgcacca gcatgtggtc
    10381 gcgacggagg cgaaattgac gaatggccag gagcgccgcg cagtccgatg cagggacatc
    10441 ggtcaagcgg cgagacatcc cggaggcttg cttccagcaa gacgccattc gtcaattgcg
    10501 ccctgccctc cgtgtcgctc agggcgggcc gcccggtgcg tcgggtggct cgagcgcagg
    10561 ccaactctac ggttttcgcc atcctcctgc cggatcagtc ccgccctcag cgatcgcgag
    10621 ccgcgcggtg gtcaatagtt cgggctagtg tgtgcgtccc gagaaatagg tgacgaagcg
    10681 ttccggagac atgggtgacg ctggcggatg gggagggatt cgaacccccg gtggagttgc
    10741 ccccacgccg gttttcaaga ccggtgcctt aaaccactcg gccacccatc cgtggcggct
    10801 cttttgtcat gaatggcggg tggtgtgaag tcaaaggcag aaggcggcga tcgccgctgt
    10861 caggtcgagc agcatcgcct cgccgcgcac ggcgaccaat acgagcgcgc cgacgatcag
    10921 cgtgccgagc gccaacatga cgaggcggcg cacgccgcgc ggcatcatcg ccgcgctcgt
    10981 ggaatcgcca gccctcgtac tgtgctcgct cacccttcgc gtcctgccac cgaaagtcat
    11041 tgccagccga ccggccggct ggcgtcaggt atatggcggg cccgccgtcg cggctcaaga
    11101 gcctccgtgg tcacgccgct gtcgccagca ccgggctacg ctcgaccggt cgctccttga
    11161 tcggccagtg aacaatcgcc gcgaacagac cgagcccgac cgagatccac cacatcgccg
    11221 tataggactt ggtgagatcg tagaggtagc cggcgagcca cacgcccagg aacgagccga
    11281 tctggtgcga gaggaatacg atgccgtaga gcatcgacat ccacgcgggg ccgaagaacg
    11341 tcgcgacgag tccgctcgtc agcgggatgg tcgacagcca gaagagcccg agcgccgccg
    11401 cgagcgcgat cacagtcact tctgtcatcg gaagatagat gaagccgaga aaaatgatcg
    11461 cgcgggcgaa atagatgagc gagaggctga tccgtttctc gacgatcttt gcggattgcc
    11521 ccgcgagcca cgtaccgagc acgttcgccg caccaactcc catcagcgcc cacgagccaa
    11581 cccaggccgg cagctgctgg tccttggcaa atgcgggaag gtgcacgccg acgaacgtga
    11641 cgtggaagcc gcatacaaaa aagcccgcga tcagcagcca gaagctcggg tgcgcgaagg
    11701 cctcagaaag tgcctctccg agcgtctggg gcttctcgcc gccgagctgc tggggcttgc
    11761 cggcaagcgg gaaggcgaac gggatgatga tcgccgtagt gaagaggacg acgagtaggc
    11821 tgccgcgcca gccgaggttc tcgatgagga agtgggtgta gggaagcgca atcaggatgc
    11881 cgaggccgct gcccatgccg accgccgaca tcgcgctcat gcgcttctcg ggaggcgcca
    11941 tgcggccaac ggcgccgaca agggcagtga cccccgtgcc cgacacgcca atgccgagca
    12001 ggaagcccga aagcatcaac tcgagcttgg tcgtcgcgat gtacatgagg tagaggccgc
    12061 ccatggtcga gagcgcgccg ccgacgatca cgcggcccgt gccgaacttg tcggagatgg
    12121 cgccggcgaa cggcgagccg atgccccaga cgatgttggc gatcgccatg gcgagactga
    12181 aggtctcgcg gccgattgtc gggtcgaatt tcgaggccat ctcggagaca aggggcacct
    12241 gatagagccc catgacgttg cgcaggccca tggccacgcc gacgacgagt gctgccgaga
    12301 ggatgacgag ccaaagcggc cgcgcggatt gggcggacat cgtgctgatc cctttctgcc
    12361 ccaggaagcg ggccggattg ctgtttgcag caagtgttat ccggtcttgg tctgccgggg
    12421 aagcgccgcc tcggcaaagg agatcgggcc ggctggcgca ggtcgcggca ccggcgggct
    12481 ccgcagttgc aacagtgcgt tgcgccgatg tagctctctc ccggcattcc aacgacagga
    12541 gagtcccatg gacatcggcg caacgcccgc cagcgagaat gagttccgca cgcgcgcgat
    12601 cagcatctac atcggcatcg cggcggtgct gctcgccatc tgcacgatgg ccggcggcaa
    12661 cgccacgaag gacgccatgc gggccaacat cgaggccgca aacacctgga gcttcttcca
    12721 ggcgcgggtc atccgacgca gcctgctcga gctctcgtcg gaagacctcg ccctcatgca
    12781 gaaatcacag cccgggcttc cggagaccgc gcagaagttg attgctgagc ggatcgccag
    12841 gaaccagtct atcgccaagg cctacacctc caatcccgaa cgccgggatg gcgccgatga
    12901 gctgttcaag aaggctcgtg agctcgaggc ggagcgcgat ctcgcgctga ggcgcgaccc
    12961 ctatttcgac tacgcgcagg cgatgctgca gatctcgatc gtactcgcct cggtggcgat
    13021 catcgcaggc ggcagcgcac tcattcaggt gagcatcggg ctcggcatga tcggtacact
    13081 gctgatgctg aacggcttca ctctgctcac caggataccg gggatcggct gaggccggcg
    13141 ggcgggcggc ctatcgagtg gtggacttgc tcgccgtctc agccctcgtc aggcttcggc
    13201 cgtgccttgc ggcagtagag ctcgaggcga tggtgcacga cctcgaagcc gagctcggcg
    13261 gcgatcttcg cttgcagctc ctcgatcttt gccgaacgga actcgatcac ggcgcccgta
    13321 tcgatatcga tgatgtgatc gtggtgcttg tcgtcagccg tctcgtagcg ggcgggtgcc
    13381 ccttcgaagc tgtggcggtg gatgacgccc cgccgctcca ggatcgccat ggtcatggtg
    13441 ttggcgatgc cgatggccgc caccagcagg gcgatggctc ccatactgcc gaaagccact
    13501 tgcagcacca ggtagaagct gctgatgccc tcgacgaacg attgcggcga ccaggcctgg
    13561 aagcccaggg cggtgatctg gtcggccacg tcgagcacct g
//
LOCUS       DAORNH010000103        13263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3528_length_13263_cov_2.783995, whole genome shotgun sequence.
ACCESSION   DAORNH010000103 DAORNH010000000
VERSION     DAORNH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3528_length_13263_cov_2.783995"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    344..430
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <431..1008
                     /locus_tag="PK264_11885"
     CDS             <431..1008
                     /locus_tag="PK264_11885"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HRD76622.1"
                     /translation="GAKDNVVTLPRRDRPAPADATAPADNSATVEAKLRGLAGGNAAL
                     GNGLVEVWRADRSFDPEAFVRGAKQAYEMVVTAFAEGNRNILKGLLSRDVFESFSSVI
                     TEREAAGHAVEQKFGISEAELAEAGVRQGVAQVAIRFVSDLISVTRDKSGAVVAGDAK
                     RTQQVIDIWTFARETSSPDPNWKLVATESPG"
     gene            1294..2445
                     /locus_tag="PK264_11890"
     CDS             1294..2445
                     /locus_tag="PK264_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HRD76623.1"
                     /translation="MTKPAGTVSLTPATFADLPGWASDDHLAAYRTFQRSCVRLIAAA
                     RTGNLAGKVATPLPLFAICQDALVKPLPKTRGAAREFFELSFAPHRVIHAGGQGLLTG
                     YYEPLLEGSRKPTDRFTAPIYKRPADLINLVHEAERGGVGANFTHARKTLKGVVPFAT
                     RAEIEKGALKGLGLELLWLADPVDVFFLQIQGSGRIRLPDGSMVRVHYDGKNGHPYSS
                     IGRYLIDRGILPADKLSMQALGRWLRSDPERAKPVMWHNASYVFFRELTGSEAESTLG
                     VLEIPLTAGRSLAVDTSHHAIGSPVYVTSPTLTHARTPNGFHRLMIAQDVGSAIRGPE
                     RGDIFFGSGDRAGGIAGITKHPGNFYVLLPRPVPSATAPATPAPPVAKR"
     gene            2613..3068
                     /locus_tag="PK264_11895"
     CDS             2613..3068
                     /locus_tag="PK264_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HRD76624.1"
                     /translation="MPAVRPAAEASAPKRTEVTVTRSAAKPPAPLPHFERREVRKLAT
                     GRREIEARLDLHGLKQVEAHARLRGFVAACARQGMSLVLVITGKGGERSGDAGDEVIR
                     SDRRGVLKRLVPLWLAEPDLRAHIVSYTEAHVRHGGGGALYVRLRKLAR"
     gene            3154..3570
                     /locus_tag="PK264_11900"
     CDS             3154..3570
                     /locus_tag="PK264_11900"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRD76625.1"
                     /translation="MPGRHLVFVYGTLKRSFPNHGYLDGQRFIGEARTVEAWPLIVQG
                     RYFSPVVLPEPGVGHRVGGEIWEVDDACLTRLDELESIGKPTGYTRDQVKVVLTNGDI
                     VEAGIYFKARDRVTIPHTGYLTDYRDCRYVASEDRE"
     gene            3567..3914
                     /locus_tag="PK264_11905"
     CDS             3567..3914
                     /locus_tag="PK264_11905"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRD76626.1"
                     /translation="MTEPPDSTDRILTALATTIRERRDASAGQSYTRSLLDGGPRRCA
                     KKFGEEAVELVIAATGEDDAAVRAEAADVLYHLLVLIEARAVPLAEVLGELARRTGVS
                     GHEEKARRPQSST"
     gene            3958..4908
                     /gene="coaA"
                     /locus_tag="PK264_11910"
     CDS             3958..4908
                     /gene="coaA"
                     /locus_tag="PK264_11910"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HRD76627.1"
                     /translation="MPASKVQFSPYDTFTRADWARLRADTPMTLVKRDLEQLSGFMEE
                     LLLDEVEQIYLPLSRLLNFYVAGMQELHSTTNKFFGRQNARMPFIVGIAGSVAVGKST
                     TARALKALLARWPDHPRVDLITTDGFLWPNKELETRGLMQRKGFPESFDTARLLNFLA
                     DVKAGRSSVAAPVYSHFHYDIVTGQSTIVDRPDILIVEGLNVFQPARMPKGGVEIPFV
                     SDFFDFSIYIDADPAIIERWYVERFMRLRRTAFRDPAAYFHRYSGLTDEEAVAKALEI
                     WRSINLKNLEENILPTRQRARLILTKGHSHRVESVAVRKL"
     gene            5089..>5378
                     /locus_tag="PK264_11915"
     CDS             5089..>5378
                     /locus_tag="PK264_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76628.1"
                     /translation="MNFRAGIGASVRDLGSVGLTPIRQAWRKFAFLDIEHGADDGSVR
                     ARQLREIVILTPWMMLGALLNCGILLYHFWRPGDRLAIGLWSVLIAGAAGYTL"
     assembly_gap    5379..5438
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5439..7871
                     /locus_tag="PK264_11920"
     CDS             <5439..7871
                     /locus_tag="PK264_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD76629.1"
                     /translation="SHAIAHATMLGGIWGSLPFCISSHADPMDHGLIIVMIVGMSASG
                     AFALATLPAAAIAHLAAMVLPFIAAMLLGVVKTSAEMPILFVNYFVVLLIIVYARYRE
                     FSQRVRDELRIARQSETISVLLKDFESTASDWLWESDSEGRLTYVSERLAQVSGIERT
                     SLLGRSIALAAGDAADAAEWRDLLAQLRLGRSIREQVVPAICSGEAAWWSLTAGPVKS
                     ASGTIVGYRGVGSDVTERRRAQAVLEMTNSKLASFNERLAREVDERTAEARSAAAAAA
                     EASQAKSRFLANMSHEIRTPINGVLGMGELLAKTELGPRQQRLLGTLNQSAKALLSII
                     NDVLDISRIEAGQMVLDRSDFDPTHAIESAIELCAGTAYLKGLEINLVFASALPDVIV
                     GDSGRLRQLLINLVGNAVKFTDQGNVTVRVSATDMGIGRAELRIKVSDTGIGISEAAK
                     SKLFAPFAQADASISRRYGGTGLGLAISHHLVSMMGGAISLESELDHGTTVTLSLPVT
                     VASWQSASGEEILDGRNLLVLDDRPQVREALASCFLGTGARVAIAGGPDDARRELHSR
                     AASLDPVEVLIVDRIAPQTDSVEFCRTLRAEDQSSSLRIVRLLSMRNVSEEARALGPD
                     GKTELLKPARRADLCAAVRSCLSTPGGLPAAREARSSAPAAPEPSRSGLKVLLAEDNP
                     VNQEVAREYLAELGCTVTVAENGRAALDALARESFDVILMDCQMPVLDGLSATRLIRE
                     GERRAGTAPIPIIAVTANAFDSDKLATRDAGMDDHLAKPFTSEELAAVLGAWCRLQPQ
                     QRSGASRQPAAA"
     gene            complement(7887..8516)
                     /locus_tag="PK264_11925"
     CDS             complement(7887..8516)
                     /locus_tag="PK264_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRD76630.1"
                     /translation="MRALGVRQVPIVSKGKAHANGQVLRDVARLCGIPYGEQKILPVA
                     ELGRRQSIILAAAQRYLAQLSEEQLKLMLPNRPRSYGQLVWHIFNVADAFLEHERGIP
                     LTAAAYNRNPAPGQDDKASIGAYGREVQAGVAKYFADVAATRDWSAKADVYYGAQTRH
                     EYLERTTWHFAQHVRQVLWRLDSLSITADGPLPADVLAGLPMPEKVWDE"
     gene            complement(8493..8591)
                     /locus_tag="PK264_11930"
                     /pseudo
     CDS             complement(8493..8591)
                     /locus_tag="PK264_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8742..9359)
                     /locus_tag="PK264_11935"
     CDS             complement(8742..9359)
                     /locus_tag="PK264_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD76631.1"
                     /translation="MSAPATLYIDYRSPFSYLIKDPVEALEREIGVNLRWLPYRVDLE
                     GAHGHNVHGRSEREWRKIRYLYADVRRIANRRSPPLVVRGTVKIFDPTLAHIAMLVAQ
                     DAGDDVFRRYHTDVQVRLWRREIDIEDRPHMRAALVAAGGDAAALDHALDSGLGEERL
                     AELQSAANAEGVFGVPMLRIHATGELFWGTDRFELFREALLAYRA"
     gene            complement(9356..10303)
                     /locus_tag="PK264_11940"
     CDS             complement(9356..10303)
                     /locus_tag="PK264_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HRD76632.1"
                     /translation="MSRHHHIRATAESNHWGAFDAAVPSILTVESGDRVTIETINGAP
                     DRLPKSGFAILPEYAEIHAHVRPRLPGHICTGPVAIRGAKPGHVLEVGIEAVELRQDW
                     GFNIVRPLAGALPDDFDNTVLTHLPLDVKRGVARLPWGDEVPLKPFFGVMGVAPPPGW
                     GAVSTVEPRRNGGNMDNKELVAGTTLYLPVHADGALFSCGDGHGAQGDGEVCITAIET
                     ALTGTFRFTLRPDMRLDWPMAETPTHAITMAFDPDLDRAARIALREMIKLIVARKGIS
                     REHAYMLCSLAADLRVTQCVNRNNGIHVMLERRYLEPRA"
     gene            complement(10326..10583)
                     /locus_tag="PK264_11945"
     CDS             complement(10326..10583)
                     /locus_tag="PK264_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76633.1"
                     /translation="MLKRMTLAVGALLAASVIAGTPAMDRAVANPPLQCKWQSFTVEN
                     GKTFLDKKCSWKYGCDCRARTCLVNGRWRARGPANCINLPG"
     gene            complement(10696..11688)
                     /locus_tag="PK264_11950"
     CDS             complement(10696..11688)
                     /locus_tag="PK264_11950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD76634.1"
                     /translation="MAERAGTRTGRDGPRAASRDSKASRSRSAEKPPAARATASKAGG
                     ERFPKRTIALVYDFDGTLSPKPMQEYTFLPKIGEDPEAFWAETNRIAREQEADGLITY
                     MHLMYKKAKAKGVRIDREDLVALGRDVELYPGVEAWFDDIAAYVRLAAESHGIELRHY
                     LVSSGLTEIIEGTRIYDRFHNVFASEYWFDPYDLPYPKRVITDTGKTQYLFRINKGVE
                     NLGHSINDHMPETARPIPFANMIYFGDGDTDVPSMAVMRKNGGHAIAVHGPGKAREKC
                     VNLFKAGRCDFFAPADYRRGSDLFKRTCLLLDRILADIRVQEEVWALGKTIDRK"
     gene            complement(11793..12437)
                     /locus_tag="PK264_11955"
     CDS             complement(11793..12437)
                     /locus_tag="PK264_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRD76635.1"
                     /translation="MKLTDLSPILVCIDLVAVAVFAVTGALVASRKQMDIVGFCWLGL
                     VTGVGGGTVRDLLIGAPVFWVRESSYVLVCIAASVAVFLTAHVPTSRYRLILWLDAIG
                     LALVTVAGAAKGLDAGAGAANAVMMGVVTATVGGIIRDIIGQEPSIILRREIYVTASV
                     AGGVALVALDRASIGRELALAAGFLTALVIRCLAIRYAWSLPTYRARPGRVPEE"
     gene            12701..13015
                     /locus_tag="PK264_11960"
     CDS             12701..13015
                     /locus_tag="PK264_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRD76636.1"
                     /translation="MKFRPLHDRVAVRRLEEETKSAGGIIIPDTAKEKPMQGEIIAAG
                     PGARDEAGKLVPLDVKKGDRVLFGKWSGTEVKIDGQDLLIMKESDILGIIEGKMSVRA
                     AA"
     gene            13057..>13263
                     /locus_tag="PK264_11965"
     CDS             13057..>13263
                     /locus_tag="PK264_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HRD76637.1"
                     /translation="MAAKEVKFSQEARERMLRGVDILANAVRVTLGPKGRNVVIEKSF
                     GAPRVTKDGVTVAKEVELEDKFENM"
BASE COUNT         2305 a         4224 c         4280 g         2307 t
ORIGIN      
        1 cggcgtgccg tcctctatgg ggtccaacga cacgaaaggc gttggcagct cggcgcggtt
       61 ggcctaattt gttcaacgtg gcatcgagat gtgatctgag gcctgcggcg ccatggtaag
      121 atttgtctcg ccgccctata tccgatgcgg tgtcgttggc gcagatcaag gcgcccgtgt
      181 cgtcgcgtga ggaagccttt ggtccggtgt tgcgtctcag acgcggctcc gaatcacagt
      241 gttccgaccc tattgggaag cccgctaccc atggacgcat tcacgatcat attcctggtc
      301 gccgccgtgg ccatcttcct gaaactcagg agttcgctcg gcannnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn ccggcgccaa agacaacgtc gtgacactgc cccggcggga tcgtccggcg
      481 cctgccgatg cgacagcgcc tgccgataac tctgccacgg tcgaggcgaa gctcaggggg
      541 ctcgcagggg gcaatgcggc actcggcaac ggcctcgtcg aggtctggcg cgccgatcgt
      601 agcttcgatc cggaggcctt cgtgaggggc gcgaagcagg cctacgaaat ggtcgtgacg
      661 gcattcgcgg aaggcaatcg caacattctg aaggggttgc tcagtcgcga cgtgttcgaa
      721 agcttcagca gcgtcatcac cgagcgcgag gcggcaggac acgccgtcga gcagaaattc
      781 ggcatcagcg aggccgaact cgccgaggct ggtgttcgcc agggtgtcgc ccaagtggcc
      841 atcaggttcg taagcgacct gatctcggtc acgcgcgaca agtcgggcgc cgtggtcgcc
      901 ggcgacgcca agcgcacgca gcaggtgatc gacatctgga ccttcgctcg cgagacgagc
      961 tcgcccgatc ccaactggaa gctcgtcgcg accgagtctc cgggctgacg ccataaacgc
     1021 cacgcaatgc tgctgcacag aagatgatgg ggtgggcatt caggctcctg ccgcgcagtg
     1081 atctggaccg gatgatgatc ggaaccgggc gatgatcaag gattgccgct cgattgcagg
     1141 cggcccgatg cgacggcaac cgtcatgtgt agggggactg tcgtgcgatt gttgcaccgg
     1201 atggcgtctg ctgctgttgt cgcgacgatt gccgctttcg gcagtctgct cgtcaccggc
     1261 ttggcggcca atcctccgtc tggagggcgg cccgtgacga aaccggctgg caccgtgtcg
     1321 ctgacgcctg ccaccttcgc cgacctgccg ggctgggcga gtgacgacca tctcgccgcc
     1381 tatcgcacgt tccagcgctc ctgcgtccgc ctcatcgccg cggcccggac tggaaatctt
     1441 gccgggaagg tagcgacgcc actgccgctg ttcgcgatct gccaggatgc actggtgaag
     1501 ccgctgccga agacgagggg cgccgctcgc gaattcttcg aattgtcgtt cgcaccgcac
     1561 cgtgtgatcc atgcgggcgg ccaagggctg ctgaccggct actacgaacc gctactcgaa
     1621 ggttcacgta agccgaccga tcggttcact gcgccgatct acaagcgccc ggccgatctc
     1681 atcaatctgg tgcacgaggc ggagcgcggc ggcgtcggtg ccaactttac gcatgcgcgc
     1741 aagaccttga aaggtgtcgt tcccttcgcg acccgtgccg agatcgagaa aggcgcgctc
     1801 aagggcctcg gcctcgagtt gctctggctc gccgacccgg tcgacgtgtt cttcctgcag
     1861 atccagggct cgggtcgcat ccgccttcct gacggcagca tggtgcgcgt ccattacgac
     1921 ggcaaaaacg ggcaccccta ctcctcgatc ggccgctacc tgatcgatcg tggtatcctg
     1981 ccggccgaca agctctcgat gcaggcgctt ggccgctggc tgcgttcgga tcccgagcgg
     2041 gccaagcctg tcatgtggca caacgcgtcc tatgtgttct tccgcgagct cacgggctcc
     2101 gaggcggaaa gcactctcgg cgtgctcgag atcccgttga cggcggggcg gagcctcgca
     2161 gtcgatacct cgcatcacgc gatcgggtca cccgtctatg tcacgtcacc gacgctcacg
     2221 catgcccgca cgccgaacgg cttccaccgc ctgatgatcg ctcaggatgt cggctccgcc
     2281 atccgcggcc ccgagcgggg cgacatcttc ttcggttccg gtgatcgggc cggcggcata
     2341 gcgggcataa ctaagcatcc gggcaacttc tacgtgctgc tgccacggcc cgtgccgagc
     2401 gcgactgcgc cggccacccc cgctccgccc gtcgccaagc gatgatgggc ggcgaccgca
     2461 aggacagacg gcagaggcag cgctccctca gcgatgcgga ggccgcactc tggtcctatg
     2521 cgacgcgcgc aacgaggccg gtcagagtca agccgcgggt taccgatgtc gaggagccgc
     2581 cccggccggc tgccgctccc ccgatctcga gcgtgccggc tgtacggccg gcggcggagg
     2641 cgtctgcacc gaagcgaacc gaggtcacgg tcaccaggtc ggcggccaag ccgcccgcac
     2701 ccctgccaca cttcgagcgc cgtgaggtcc gcaagctcgc aactggccga cgcgagatcg
     2761 aggcgcggct cgatcttcac ggcctgaagc aagttgaggc acatgcgcgc ctcaggggtt
     2821 tcgtcgccgc ctgcgcacgg cagggcatga gcctcgtgct ggtgatcact gggaagggcg
     2881 gagaacggag cggggacgcc ggcgacgagg tgatccgttc cgatcggcgc ggtgtcctca
     2941 agaggctggt gccgctctgg ctcgcggagc cggacctcag ggcccatatc gtgagctaca
     3001 cggaagctca cgtccgccat ggcggcggcg gggcgctcta cgtgaggcta cgcaagcttg
     3061 cccgctaatg gtcccgtgag ctttcccgtg gacaagaccg cgccaagggc tgaggatgcg
     3121 cgccgcgctt gccgcgataa caggggtgcg gacatgcccg gacgccatct cgtcttcgtc
     3181 tacggaactt tgaagcggtc gtttccgaac cacggctacc tcgacgggca gcgcttcatt
     3241 ggtgaagcgc gcaccgtgga ggcctggccg ctcatcgtac aaggccgcta cttttctcct
     3301 gtcgtgctac cggagcccgg cgtcggacat cgcgtcggcg gcgagatctg ggaggtggat
     3361 gacgcctgcc tcactcggct cgacgagctc gagagcatcg gcaagcctac gggctatacg
     3421 cgcgaccagg tgaaggtcgt cctgaccaat ggcgacatcg tcgaggccgg catctacttc
     3481 aaggcgagag accgcgtgac gatccctcac accggttacc tcaccgacta ccgcgactgc
     3541 cgatacgtcg cctccgagga ccgcgaatga ccgagccgcc ggattcgacc gaccgcatcc
     3601 tgaccgctct ggccacgacc ataagggagc gccgcgatgc ctccgccggg caatcctata
     3661 cccgctcact gctcgatggc ggaccgagga ggtgcgccaa gaagttcggc gaggaggcgg
     3721 tggagctcgt catcgcagcg acgggcgagg acgatgcggc ggtcagggcc gaggcagcag
     3781 acgtgcttta tcacctcctc gttctcatcg aggcgcgagc cgtcccgctc gcagaggttc
     3841 tgggcgagct cgcccggcgg accggcgttt caggccacga ggagaaggcc cgccgtccgc
     3901 aatcgagcac ctgaccctgg tcagcggacc tccccacgac gcgcagagat cagagcgatg
     3961 ccggcctcga aagtccagtt ctcaccctac gacaccttca cgcgggccga ctgggcgcgc
     4021 ctgcgcgccg atacgccgat gacgctcgtg aagcgcgatc tcgagcaact gtcggggttc
     4081 atggaggaat tgctgctcga tgaggtcgag cagatctacc tgccgctgtc ccgcctcctg
     4141 aatttctacg tggctggcat gcaggagctc cattcgacga ccaacaagtt cttcggccga
     4201 cagaatgcgc gcatgccgtt catcgtcggc attgcagggt cagtcgccgt cggcaagagc
     4261 acgaccgcgc gcgctctcaa ggctctgctc gcacgctggc cggatcatcc gcgcgtcgac
     4321 ctcatcacga ccgacggctt cctgtggccg aacaaggagc tcgaaacgcg cgggctcatg
     4381 caacgcaagg gcttccccga gagcttcgat accgcgcggc ttctcaattt cctggctgat
     4441 gtgaaggccg ggcgatcatc cgtggccgcg ccggtctatt cccacttcca ttacgacatc
     4501 gtcaccggcc aatcgaccat cgtcgaccgc cccgacattc tgattgtcga ggggctcaat
     4561 gtctttcagc cggcacgaat gccgaaggga ggggtcgaga ttccattcgt ttcggatttc
     4621 ttcgacttct cgatctatat cgatgccgat cccgccatca tcgaacgctg gtacgtcgag
     4681 cgcttcatgc gcctcaggcg gacggcattc cgcgatccgg ccgcctattt ccaccgctac
     4741 tccggcctga cggatgaaga ggcggtggcc aaggcgctcg agatctggcg ctcgatcaat
     4801 ctcaaaaatc tggaggagaa tatactaccg acacgtcaac gggcacgcct catcctcacg
     4861 aaaggtcatt ctcatcgtgt cgagagcgtt gcggttcgga aattgtgaag gattatcgag
     4921 cgttggacat gaacgcgggc ttaccgcccc attttcagtt gtttcggcca attgctctca
     4981 ttaagtcaat caattgtaaa gtatagccgc ctacgctcga tgccagccga tcttccttga
     5041 tgtgatcggt cgctgcaggc cgcagctgcg gttcgatcgg gggtgcgtgt gaactttcga
     5101 gccggaattg gagcgtccgt tcgcgacctc ggctccgtcg ggctgacgcc gatcaggcag
     5161 gcctggcgaa aattcgcgtt cctcgatatc gagcatggcg ccgatgacgg ctcggtgagg
     5221 gcacggcagc tccgtgagat cgtaatcctg acgccgtgga tgatgctcgg cgcactcctg
     5281 aactgcggca ttctactcta tcacttctgg aggccagggg accgtctggc gatcggcctg
     5341 tggtccgtcc tcattgccgg cgcggccggc tacacactnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ccacgccatt gcccacgcga
     5461 ccatgctcgg cggcatctgg ggcagcctgc cgttctgcat atcctcgcat gccgatccga
     5521 tggatcatgg actcatcatc gtgatgattg tcggcatgtc ggcgagcggc gcgtttgcgc
     5581 tcgcgacact gccggccgct gccattgcgc acttggccgc gatggtgctg ccattcattg
     5641 cggccatgct gcttggcgtc gtgaagacgt cggcggagat gccaatcctc ttcgtcaact
     5701 acttcgtggt cctgctgatc atcgtctatg cgcgctaccg cgaattcagc cagagagtgc
     5761 gtgatgagct gaggatcgcg cggcagagcg agacgatctc tgtgctgctc aaggacttcg
     5821 agagcactgc cagtgactgg ctctgggaga gtgattcgga aggccggctg acgtacgttt
     5881 cggagcggct ggcgcaggtt tccggaattg aacgaacgtc gcttctaggt cgctcgatcg
     5941 ctctggccgc gggtgacgcc gctgatgccg ccgagtggcg cgacctcctc gcccagcttc
     6001 ggctcgggcg ctcgatccgg gagcaggtcg tgccggcgat ctgcagcgga gaggccgcct
     6061 ggtggtcgtt gaccgcgggc ccggtgaagt cagcgtccgg caccatcgtc ggctaccgtg
     6121 gtgtcggttc tgacgtcacc gagcggcgac gggcgcaggc cgttctcgag atgacgaact
     6181 caaagctcgc ctccttcaac gagcggctcg cgcgcgaggt ggacgagcgc acagccgagg
     6241 cgcggagcgc cgcggcggcg gcagccgagg ccagccaggc gaagtcgcgc ttcctcgcca
     6301 acatgagtca cgagattcgc acgccgatca atggcgtgct cggtatgggc gagttgcttg
     6361 ccaagaccga actcggcccg cgccagcaac gcctgctcgg cacgctcaat cagtcggcga
     6421 aggcgctgct ctcgatcatc aatgacgtac tcgacatctc gcggatcgag gccgggcaga
     6481 tggtgctcga tcggtccgac ttcgatccga cgcacgcgat cgagagcgcc atcgagctgt
     6541 gtgccggcac agcgtacctc aaggggctcg agatcaacct cgtcttcgcc agtgccttgc
     6601 ctgacgtgat cgtcggtgac agcggacgac tgaggcaact gctcatcaat ctcgtcggca
     6661 atgccgtcaa attcaccgac cagggcaatg tgaccgttcg tgtctcggcg accgacatgg
     6721 gcatcgggcg ggccgagctt cgcatcaagg tctcggacac cgggatcggc atcagcgagg
     6781 cggcgaagtc gaagttgttc gcgcccttcg cgcaggccga cgcatcgatc agccgccgct
     6841 acggtggtac ggggctcggc ctcgccatct cgcaccatct cgtgtcgatg atgggcggag
     6901 cgatctcgct cgagagcgag ctcgatcacg gaaccacggt gacgctgtcg ctgcctgtga
     6961 cggtggcgtc ctggcaatcc gcgtcgggcg aggagatact cgacggccgc aacctcctcg
     7021 tactcgacga tcgccctcag gtgcgcgagg cgctggcatc atgttttctc ggcacgggtg
     7081 cgcgcgttgc gatcgcgggc ggaccagacg acgcgcgccg tgagctccat tcccgcgccg
     7141 cgtccctcga cccggtcgag gtgctgatag tcgaccggat cgcgccccag acggacagcg
     7201 tcgaattctg caggacactg cgcgcggagg atcagtcgag cagtttgcgc atcgtgcgcc
     7261 tcctgtcgat gaggaacgtc tccgaagagg cacgggcgct agggccggac ggcaagaccg
     7321 agcttctgaa gccggcgcgc cgcgccgatc tgtgtgccgc ggttcggagc tgtctctcga
     7381 cgccgggcgg cttgcctgcg gcgcgtgagg cccgatcctc cgcgccggcc gcccccgagc
     7441 cgagccgttc agggctcaaa gtgctgctcg ccgaggacaa tcccgtcaat caggaggttg
     7501 cgcgtgaata cctcgccgag ctcggctgca cggtgacggt cgccgagaat ggccgcgcgg
     7561 cgctcgacgc gttggcgcgg gagtcgttcg acgtgatcct gatggactgc cagatgcccg
     7621 ttctggatgg tctctccgcc acgcgtctca ttcgcgaggg tgagcgacgc gccggaacgg
     7681 cgccgatccc gatcatcgcg gtgacggcca acgccttcga cagcgacaag ctggcgacgc
     7741 gagacgccgg catggacgat cacctcgcca agccgttcac gtcagaggag ttggcagccg
     7801 tactcggcgc atggtgccgg cttcaaccgc agcaacggtc gggtgcatcg cgacagccgg
     7861 cagcggcctg aggtctgcag gcgtcgctac tcgtcccaga ccttctcagg catcggcagc
     7921 cctgcgagaa catcggccgg cagcggaccg tcggccgtga tgctgaggct gtccaatcgc
     7981 cagagcacct ggcgcacgtg ctgcgcgaag tgccatgtcg tgcgctcgag gtactcgtgg
     8041 cgcgtctgcg caccatagta gacgtccgcc ttcgccgacc aatcccgcgt cgcggccacg
     8101 tctgcgaagt acttggccac gcccgcctgg acttcgcggc cataagcgcc gatcgaggcc
     8161 ttgtcgtctt ggccgggggc gggattgcgg ttgtaggccg ccgctgtcaa tggaatgccc
     8221 cgctcgtgct cgaggaaggc gtcggcgacg ttgaagatgt gccagacgag ttggccgtag
     8281 ctccgcggcc ggttcgggag catcagcttg agctgctcct cggaaagctg ggcgaggtag
     8341 cgctgtgctg ctgcgagaat gatcgactgg cggcggccga gctcggcgac gggcaagatc
     8401 ttctgctcac catacgggat gccgcagagc cgcgccacgt cgcgcagcac ttgcccgttg
     8461 gcgtgcgcct tgcccttgct gacgattggc acctgccgga cgccgagcgc gcgcatctcc
     8521 tcaagcgcgc cctcatccgc gaggacgttg cgagaaacga acggtatccc atgccgccca
     8581 aggaactcct tggttctaag acagctcgtt caaccaggct gccagtagac gcggacctcg
     8641 agtgcagcgt cgctcacgct cgtctccatg acttgatgag gcggatcgta gctcggcccc
     8701 acgcattccg caacgcgttg ccgacacgcg ttgccagcat atcaggcgcg gtacgcaagc
     8761 agtgcctcgc ggaagagctc gaagcggtcg gtgccccaga agagctcgcc cgttgcgtgg
     8821 atgcgcagca ttggcacgcc gaacacgcct tcggcattag cggcggactg caactcggcg
     8881 aggcgctctt cgccgagacc gctgtcgagc gcatgatcga gggcggcggc atcgccaccc
     8941 gccgcgacga gtgcagcccg catgtgcggg cgatcctcga tatcgatctc gcgtcgccag
     9001 aggcgcacct ggacgtcagt gtggtagcgg cggaagacat cgtccccggc gtcttgagca
     9061 acaagcatcg cgatatgcgc cagcgtcgga tcgaagatct ttaccgtgcc gcggaccacc
     9121 agtggcggcg agcggcgatt ggcgattcgg cgcacgtcgg catagaggta gcggatcttg
     9181 cgccattcgc gctcgctgcg gccatggacg ttgtggccgt gcgcgccttc gagatcgacg
     9241 cgatagggca gccagcggag attcacgccg atctcgcgct cgagtgcctc gacggggtcc
     9301 ttgatcaggt aggaaaaggg gctgcgatag tcgatataga gcgtcgctgg cgcactcatg
     9361 cacgaggctc gaggtagcgg cgctcgagca tcacgtggat gccgttgttg cgattgacgc
     9421 attgcgtgac gcgcagatcc gcggcaagcg agcagagcat gtaggcgtgc tcgcgcgaga
     9481 tgcccttgcg cgcaacgatc agcttgatca tctcgcggag cgcgatacgt gccgcgcgat
     9541 cgaggtcggg atcgaaggcc atggtgatgg cgtgcgtcgg cgtctcggcc atcggccagt
     9601 cgaggcgcat gtcaggccgc agcgtgaagc ggaatgtgcc ggtgagcgcg gtctcgatcg
     9661 ccgtgatgca gacttccccg tcgccttgcg caccgtggcc gtcgccgcac gagaagagcg
     9721 cgccgtcggc atggaccggc agatagagtg tggtgccggc gacgagctcc ttgttgtcca
     9781 tgttgccgcc attgcggcgc ggctctaccg tagagaccgc gccccatccg ggcggcgggg
     9841 cgacgcccat tacgccgaag aagggcttga gaggcacttc gtcaccccag gggagccgcg
     9901 cgacgcctcg cttcacgtcg agcggcaggt gggtgaggac cgtgttatcg aagtcgtcag
     9961 ggagcgcgcc ggcaagcggg cggactatgt tgaaacccca atcctgccgg agctcgaccg
    10021 cctcgatgcc gacctcgagc acgtgtcccg gcttggcgcc cctgatcgcc acgggaccag
    10081 tgcagatatg tccggggaga cgcggccgga catgcgcgtg gatctcggca tactcgggca
    10141 ggatcgcgaa gccggatttc ggcagccgat ccggcgcgcc attgatggtc tcgatcgtca
    10201 cgcggtcgcc gctctcgaca gtgaggatcg acggcaccgc tgcgtcgaac gcaccccaat
    10261 ggttcgactc agccgttgcg cggatgtggt gatggcggct catgtcttgc gatccaaggc
    10321 gctgctcatc ccggaaggtt tatgcagttc gcaggccccc tcgcccgcca gcggccattc
    10381 acgaggcacg tccgcgcgcg gcagtcgcag ccatacttcc aagagcactt cttatcgagg
    10441 aacgtcttgc cgttctcgac ggtgaacgac tgccatttgc actgcagcgg cggattggcg
    10501 acggcacgat ccatcgcggg cgttccggcg atgaccgagg cggctagcaa ggcgccaacg
    10561 gcaagtgtca ttcgcttcag catcgagaca atctccaaac tcggtttgat cacgacccag
    10621 cagaggaaga ggctagaact ccgggccgat cgggtcaaac tcgatccagc atcaggcttg
    10681 ccgcggccac ccgcatcact tcctgtcgat cgtcttccca agcgcccaaa cctcctcctg
    10741 gacacggatg tcggcgagga tgcggtcgag caataggcag gtgcgcttga agaggtcgga
    10801 gccgcgtcga taatcggcgg gcgcgaagaa gtcgcagcgg cctgctttga agagattgac
    10861 gcacttctcg cgcgccttgc cgggtccatg gaccgcgatg gcatgaccgc cgttcttgcg
    10921 catcacggcc atgctcggca cgtcggtgtc gccgtcgccg aagtagatca tgttggcgaa
    10981 ggggatcggc cgcgcggtct ccggcatgtg atcgttgatg ctgtgaccga ggttctcgac
    11041 gcccttgttg atgcggaaca ggtactgcgt cttgccggtg tcggtgatga cccgcttcgg
    11101 atacggcaag tcgtaggggt cgaaccagta ttccgacgca aacacattgt ggaagcgatc
    11161 gtagatgcgc gtcccctcga tgatctcggt caggcccgaa gagacgaggt agtggcggag
    11221 ctcgatgccg tggctctccg cggcgaggcg gacatacgcg gcgatgtcgt cgaaccaggc
    11281 ctcgacgccc ggatagagct cgacatcgcg gccgagggcg acgaggtctt cccggtcgat
    11341 gcgcacgccc ttggccttcg ccttcttata catgagatgc atataggtga tgaggccgtc
    11401 tgcctcttgc tcgcgcgcga tgcggttggt ctcggcccag aaggcctccg ggtcctcgcc
    11461 gatcttgggc aggaaggtgt actcctgcat cggcttcggc gagagcgtgc cgtcaaagtc
    11521 gtagacgagc gcgatggtgc gcttgggaaa gcgctcgccg cctgccttgc tcgctgttgc
    11581 ccttgcagcc ggcggcttct ccgcgcttcg gctccgcgaa gccttgctgt ctctcgatgc
    11641 tgcgcgcggg ccatcacgac ccgtgcgtgt tcccgcccgc tcggccatgg cgcgatcctc
    11701 gttcctcaac ctggatatca tcagcaactc aatcacgatt cgcgaggcag aacggtggcc
    11761 aagcacgagc tatcaacgga aacccggtcc gatcactcct ccggtacgcg gcctggacgc
    11821 gcgcgatagg tcggcagtga ccaggcgtag cggatcgcga ggcagcggat caccaaggcc
    11881 gtcaaaaatc ccgcggccag cgccagctcg cgcccgatgc tggcgcggtc cagggcgacg
    11941 agcgcgacgc cacccgcgac cgacgcggta acatagatct cgcggcgcag gatgatcgac
    12001 ggctcctggc cgatgatatc gcggatgatg ccgccgacgg tcgccgtcac cacgcccatc
    12061 atcacggcat tggccgcgcc ggcgccggcg tcgaggcctt tcgccgcgcc ggcgacggtg
    12121 acgagcgcaa gcccgatcgc gtcgagccag agtatgagcc gatagcgaga ggtcgggacg
    12181 tgcgccgtga gaaagacggc aaccgaggcc gcgatgcaca cgagtacata gctcgattca
    12241 cgcacccaga agaccggcgc gccgatcagg aggtcccgga cggtgccgcc gccgacgccg
    12301 gtgacgaggc cgagccagca gaagccgacg atatccatct gcttgcgcga ggcgacgagc
    12361 gcacctgtca ccgcaaagac ggcgacggcg acgagatcga tgcagacgag gatcggcgac
    12421 aggtcagtca acttcatggt ttcccccaca ggcccgacac acatgtccgg cgcccgtgga
    12481 tggcttatcg ctaccacgat cggtcgtcac catcggcgcg acaggcactg tcagactggc
    12541 actcgaccct tgacagtgct agcagatcac gacatataac ccgtcaaggc cggcagcgat
    12601 ccgtcctgat gggcgttaac ttattgatcg cacctgcttt atccttctgc gcactccgtg
    12661 cacaggcagc tgacaaccag gcaattctcg gaggagaaac atgaagtttc ggcccctgca
    12721 cgaccgcgtc gccgtgcgcc gcctcgaaga agagaccaag tccgccggtg gcatcatcat
    12781 tcctgacacc gccaaggaaa agccgatgca gggggagatt atcgctgccg gcccgggcgc
    12841 ccgcgacgag gccggcaagc ttgtgccgct cgatgtgaag aagggcgatc gcgtcctctt
    12901 cggcaagtgg tccggcaccg aggtgaagat cgacggccag gatctcctca tcatgaagga
    12961 gagcgacatc ctcggcatca tcgaaggcaa gatgtcggtt cgcgccgcgg cctgatcgca
    13021 ggctgagttg caattcaaga tccaggaaac cgcacaatgg cagccaaaga agtcaagttc
    13081 tcccaggaag ctcgcgagcg catgctgcgc ggcgtcgaca tcctcgctaa cgctgtccgc
    13141 gtgacgctcg ggccaaaggg ccgcaacgtc gtcatcgaga agagcttcgg cgccccccgc
    13201 gtgaccaagg acggcgtgac tgtcgccaag gaagtcgaac tcgaggacaa gttcgagaac
    13261 atg
//
LOCUS       DAORNH010000104        13214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3542_length_13214_cov_2.580363, whole genome shotgun sequence.
ACCESSION   DAORNH010000104 DAORNH010000000
VERSION     DAORNH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13214
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3542_length_13214_cov_2.580363"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PK264_11970"
     CDS             <1..1058
                     /locus_tag="PK264_11970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD76638.1"
                     /translation="VGIIVDSVASDPLTERRLAEIAPALSLGILAVPMSREIGLSDYR
                     AFHLIRRRALALQCDVLHGHGAKGGAFARLVAQSLIRSGRPVIAAYTPHGGSLHFHPS
                     TLKGRVYMALERYLAGLTHGIVFESAYSAETFASHVGTPHCPARVIPNGLADTDFEPV
                     PHDSDAADVLFVGELRHLKGVDILLEAVARLPEARAVIVGAGPDRAQFEADVLRLGIS
                     SRVTFAGAMPAREAFRRGRILVMPSRAESFPYIALEAAAAALPLVATSVGGVPEIVAG
                     TDTRLVPPGDASALAEALRIAIAEPDLMRARAERLRASVASRFTVARMADGVGALYAA
                     TGRTPLKGAPRSIPRPA"
     assembly_gap    1134..1154
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1169..>2414
                     /locus_tag="PK264_11975"
     CDS             1169..>2414
                     /locus_tag="PK264_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRD76639.1"
                     /translation="MQISPAASGPIVDLPRLADLRAFDKSRLISPAVVSGLVRFGEFV
                     VIGLLGLAIAFGYLGRVEVLESARYLAAVVLTGAVTIVAAHALETYATPLLSSPVRAM
                     PRLLLAWMSAFGVLLTVMFLLKTGLEFSRVWLMVWFVAGAIGLAGWRFAVSAAIRSLM
                     RQGRLNRRAVIYGGGPACEALIKALETDQNGDILIAGIFDDRGERVASKVAGYPKLGS
                     VEDLINFCRFTRVDLLLVSLPVTAEERLLQILRKLWILPVDIRLTAHTTKLRFRPRAY
                     SYIGSVPMIDLFDKPIADWDNVIKSAFDRVVAAVALVALAPVMALVGLAVRLDSKGPV
                     LFRQKRYGFNNELIEVWKFRSMYVEQSDVDAARLVTRGDPRVTRVGRFIRKTSLDELP
                     QLFNVLGGTLSLVGPRPHALQAK"
     assembly_gap    2415..2455
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2456..2651
                     /locus_tag="PK264_11980"
     CDS             <2456..2651
                     /locus_tag="PK264_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRD76640.1"
                     /translation="RHKVKPGITGWAQINGWRGETDTPEKIQKRVEHDLYYIENWSLV
                     LDVYILLKTPLSLLSAKNAY"
     gene            2749..4005
                     /locus_tag="PK264_11985"
     CDS             2749..4005
                     /locus_tag="PK264_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76641.1"
                     /translation="MRSMTSSALPAMGLSDRIAAVDLTRRQLGAAGVAFAIGTSAVVF
                     SEPAPTDLVMMALIPALPLLGLVRVTPALLAYLALWLVCGAGAVVAATFSQDVSRSSV
                     HSAVSIYLYLASFVLAGYIAADPVRRTELVLRAYVVAAGVAAAAALAGYFALVPWAYD
                     LFTKFGRASGTFKDPNVLGPFLVPAVLYLVHRAVSEPAQKMAGPLAMALVLVVVVVLS
                     FSRGAWLNLAVALLVFGYLHFVTARSDRHRLRLVVLAFTGLVAIAAIIVVALQFDGVG
                     RLLAERASLTQSYDVGPDGRFGGHDKARALLLENPLGIGALQFAARFHHEDVHNAYLN
                     MFLSAGWIGGLLFIIILVGTMLLALRHAIGRAEVSPLFLIAVAALAGNVVEGFVIDID
                     HWRHLYLLLAVAWGFMTARTGERASG"
     gene            4091..5497
                     /locus_tag="PK264_11990"
     CDS             4091..5497
                     /locus_tag="PK264_11990"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRD76642.1"
                     /translation="MSDIRLHETLRSPGPVRRLALLLVWVTVASGAVVFAEPAPADFL
                     MMGLIVLLPVIGLVAIRREHIAYLSLWLIVAACGFAAAFIAENVERATTHTAITLYLA
                     IASFMVAAFVARNPARHARLILGAYTFAATLTSLFAIIGYFGLIPEATAWFTLHQRAS
                     GTFKDPNVFGPFLVPAIIYALHRWIERPARRAMLALLVLPVLIVAMLLTFSRGAWLAL
                     ALSLAVFGYASFITAATNRHRLKLMVLAAFGVAALAGVVASALQIDGVARLFDQRAAL
                     TQSYDIGPEGRFGGQDKAIALILAHPFGIGAREFGGRMHPEDPHNVYLSMLLNAGWLG
                     GLTYALIIVLTIAFLARALMRATATRPELVVVLASLAGMASLGFFVDQDHWRHLYLLL
                     GVGWGLMTGDRRLLRPARVMVARRLVPEHLGLQISRRRARIIGPTLRPMPTLVIAPTV
                     TRRTARRLRRSARILGPA"
     gene            5576..6532
                     /locus_tag="PK264_11995"
     CDS             5576..6532
                     /locus_tag="PK264_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRD76643.1"
                     /translation="MSQSCRFPRTASTIAAAALWLAVAGTARGAPPEVIATVKPVHSI
                     AAAVMDGVGVPRLLIDGAASPHTYALKPSDAKALNGARLIIRVSPGLETFLDSTLKSL
                     PKSVRVLSLDRAEGMVLLAVRTGANFDGDDHGHGHGHGKSKSAAMDAHIWLDPRNAAA
                     IAGAVAGALSDIDPANAEAYRTNAKRASDKFANLDRELESLARPLAGKPFVVFHDAYQ
                     YAEKRYGLTAVGAITVSPEIQPSAKRLQALRRTIQRLHAHCVFVEPQFPPKLIDTIIE
                     GSKARRGTVDPLGVAIPAGPDHYATLMRTLIADFAACLAPRS"
     gene            complement(6614..6853)
                     /locus_tag="PK264_12000"
     CDS             complement(6614..6853)
                     /locus_tag="PK264_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76644.1"
                     /translation="MKRWILGLGLGVALSFAVPFGMSVSDSEAKPKYRNKMCMYKGPI
                     SGKASNWKCKIDEVCCWSALENKGSCRPKGTICAL"
     gene            complement(7014..8186)
                     /locus_tag="PK264_12005"
     CDS             complement(7014..8186)
                     /locus_tag="PK264_12005"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HRD76645.1"
                     /translation="MSTSSTRPAAAQGKAQRGVSETGDPLLLTPGPLTTSKRVKEVMV
                     HDWGSRDAAFLAINKAVLERLVDIVGGKEHGFVTVPVQGSGTYAVEAMLTSFVPADGG
                     CLVLVNGAYGQRAKRILDIARRHAVVHETAEDCPPDLAAVEKLLAADKRLTHVFAVHC
                     ETTSGILNPIREIGAIAERHGRRFLLDSMSAFGALPLDVTAGFDAVAASSNKCIEGIP
                     GLGFVIASESALARTKGNATSLTLDLHDQWQNFAKTGQYRFTPPIHVIVSFHQALEEF
                     FAEGGQAGRGGRYVANGRVLLEGMRALGFRPLLSDNLQAPIIYTFHMPTNAAFKFQAF
                     YDRLKERGYVIYPGKLTVADSFRIGCVGRIDEGHMRAFIANVREVLAEMGVGRIDA"
     gene            complement(8229..10328)
                     /locus_tag="PK264_12010"
     CDS             complement(8229..10328)
                     /locus_tag="PK264_12010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRD76646.1"
                     /translation="MPRKSSDRSAGKAATSPRAADNPLLGAWSAPFAMPPFEAVRLEN
                     YKPAFAAAMREHLKEIAAITKSESRPTIANTIDALERSGRLLSRVGGIFYNLTGTVST
                     PELRAIERELAPLMAQHRNRIMLDAKLFQRIDDLHSRRARLKLDDEQHRVLERTHRNL
                     VRAGAALPPAKKKRVAAINERLATLATSFAQNVLADEQAFKLILDGESDLAGLPDFAR
                     AAAKRLAADLGHEGKHAVTLARSSIETFLQFSARADLRAKAFDAWSRRGEMGGASDNR
                     EIITETLKLRAERAALLGYPTHAAFSLDDTMAKTPESVDGLLGEVWTAAKRKVGEERE
                     ALLAEARKEGSNAPLRAADWRYWAEKVRKARYDFDESELKPYLQLERMIEAAFDVAGR
                     LFGLKFRELPDAPRYHPDVRVYEVRDKRGRHIGVFMGDYFARPTKRSGAWKSAIRSQW
                     KLDGEVRPIILNVLNCAPPGEGQPALLSYDDARTLFHEFGHALHGLLSDVTYPSIAGT
                     AVARDFVELPSQLYEHWLGTREVLEKHAVHVTTGQPMPAKLLAKLKAARAFNQGFSTT
                     EFIASAMADMELHKCKPDELPADLGRFEAELFARIGMPAEVTMRHRLPHFQHIFSGGY
                     ASGYYSYMWSEVLDADAFSAFEEAGDVFDAKTAKKLKDHIYSAGNRRPPEEAYVKFRG
                     RLPTPDAMLAKRGLKTT"
     gene            complement(10383..12062)
                     /locus_tag="PK264_12015"
     CDS             complement(10383..12062)
                     /locus_tag="PK264_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRD76647.1"
                     /translation="MGGSEVLLTLLGAVALLLWGVRMVRTGLTRAFSGPLRALLGEAS
                     RNRMTAFLAGLGVTAVLQSATATSLLLSSFAGRGLITLPLALAVMLGADVGSTLVAQL
                     FSLDIRWLWPVLMFGGVVAFNAARGERAKSVGRILIGLGLMLLALGVIANVSGAIRES
                     QAMAVLLSALGSELIVAAVVAALVTWLAHSSLAIILFVMSLAASGVIKPALALALVVG
                     ANVGGAIAPLTALTGAPTAARRVPVGNLIARVVIAVVMLPFVQRLVPLLEYASAVPSQ
                     LVLNFHCLFNVAVAAAFLPLLRPLAEGVERLLPDPKLAPDPRLPQHLDPSALDSPSEA
                     MGCALRETLAVGNIVHDMLRRSLTAIETNDTKLIKEIERTDDHVDALHEAIKLYMIRA
                     SKTDMDEGESRRYVEILTFTTNLEHIGDIIDKNLMELAQKKAKKGYAFSSEGLAELKT
                     FHAQVLENMRLALNVFALRDVALARRLLLAKTDMRASEFDAANRHFARLKAGRQESIE
                     TSSIHLDIVRDLKRINSHLTSVAYPILEAAGELNETRLKLERGVAAAPETA"
     gene            12316..>13214
                     /locus_tag="PK264_12020"
     CDS             12316..>13214
                     /locus_tag="PK264_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD76648.1"
                     /translation="MRLVAALFFLLSVLAAEPAHAQGKRVALVIGNEAYAHASALKNP
                     VRDAAAVAAALKRIGFELVEVQRNLGVDALRRALLAFEGHATGADIALVFFAGHGIEV
                     DGVNYLVPTDARLTRANGVELEAVALPTVTAAISGARKLRLVILDSCRNNPFQPRLAA
                     DSGRKRSIGRGLARVEPGDNELIAFAAAAGTTADDGEAGNSPFTTALLKHLERPGLEV
                     QQLFRNVRDEVIASTARGQVPHVYGTLGAEPVFFRAALASVDTVKANEAEIARRVAAE
                     RARLEAEMAARLEAEKRRLAAATP"
BASE COUNT         2032 a         4498 c         4293 g         2329 t
ORIGIN      
        1 aagtcggcat catcgttgat tccgttgcga gtgacccgtt gacggagcgc cgcctcgccg
       61 agatcgctcc cgcgctgagc ctcggcatcc ttgcggttcc catgagccgc gagatcgggc
      121 tcagcgacta ccgcgccttc cacctgatcc gccgccgcgc cctcgctctg caatgcgacg
      181 tgttgcatgg ccacggtgcg aagggcggcg ccttcgcacg tctcgtcgcg cagtcactca
      241 tccgctcagg acggcccgtg atcgcggcct ataccccgca cgggggcagc ctgcatttcc
      301 acccttcgac cctcaaaggg cgcgtctaca tggcgctgga gcgctatctc gccggcctca
      361 cccacggcat cgtgttcgaa agcgcctact cggccgaaac cttcgcgtcc catgtcggga
      421 caccccattg cccggcccgc gtgatcccga acggcctcgc ggacaccgac ttcgagccgg
      481 tcccacacga cagcgatgcc gccgacgtgc tgttcgtcgg cgaactccgc cacctcaagg
      541 gcgtcgacat cctgctcgag gccgtcgcgc ggcttcccga ggcgcgcgcc gttatcgtcg
      601 gtgccggccc cgaccgggcg cagttcgagg ccgacgtcct gcgtctcggg atttcctcgc
      661 gcgtcacctt cgcgggcgcg atgcccgcca gggaggcctt ccgccgcggc cgcatcctgg
      721 tgatgccgtc gcgcgcggag agtttcccct atatcgcgct cgaggcggcc gccgccgcgc
      781 tgccgctggt cgcgacctcg gtcggcggcg tccccgagat cgttgccggg acggatacca
      841 ggctcgtccc cccgggagac gcgagtgccc ttgccgaggc gctgcggatt gcaatcgcag
      901 agccggacct gatgcgcgcc cgtgccgaac ggctccgtgc ctcggtcgcc tcccgcttca
      961 cggtcgcccg gatggccgat ggcgtcggcg cgctctatgc ggcgaccggg cgcacgcccc
     1021 tcaagggtgc cccgcgctcc atcccgcggc cggcctgatc gattgcgatc gtggacaccg
     1081 cttcctaacg gcacacttca taatatctgt cttggcacgt ctcctgcttg agcnnnnnnn
     1141 nnnnnnnnnn nnnnggttga ccgctgacat gcagatctcg cccgccgcct ccggaccaat
     1201 cgtcgacctt ccccgcctcg cggatctccg cgccttcgac aagtcgcgcc tcatctctcc
     1261 ggcggtcgtc tcagggctcg tgcgcttcgg cgaattcgtc gtgatcggcc tgctaggcct
     1321 cgccatcgcc ttcggctatc tcggccgcgt cgaggtgctt gagagtgcgc gctacctcgc
     1381 ggccgttgtc ctgaccggcg ccgtcaccat cgtcgccgcc cacgccctag agacctacgc
     1441 cacccccctc ctttcctcgc ccgttcgcgc gatgccgcgg ctcctgctcg cctggatgtc
     1501 ggcgttcggt gtcctcctca ccgtgatgtt cctcttgaag accggcctcg agttctcgcg
     1561 cgtctggctc atggtctggt tcgtcgcagg cgccatcggt ctcgccggct ggcgcttcgc
     1621 agtctccgcc gcgatccgct cgctgatgcg ccagggccgc ctcaatcgcc gcgccgtgat
     1681 ctacggcggc ggcccggctt gcgaagcgct gatcaaggcg ctcgagaccg accagaacgg
     1741 agatatcctc atcgccggca tcttcgatga ccgcggcgag cgcgtcgcga gcaaagtcgc
     1801 gggctatccg aagctcggca gcgttgagga cctgatcaat ttctgccgct tcacgcgcgt
     1861 cgatctgctg ctcgtgtcgc tgcccgtcac ggccgaggag cggctcctgc agatcttgcg
     1921 caagctctgg atcctgccgg ttgacatccg tctgactgcg cacacgacca agctgcgctt
     1981 ccggccgcgc gcctactcct atatcggctc ggtgccgatg atcgacctct tcgacaagcc
     2041 gatcgcggat tgggacaacg tcatcaagtc ggcattcgac cgtgtcgtgg cggccgtcgc
     2101 cctcgtggcc ctagccccgg tgatggcact cgtcggcctt gccgtccgcc tcgacagcaa
     2161 ggggccggtg ctcttccgcc agaagcgcta cggcttcaac aacgagctga tcgaggtctg
     2221 gaagttccga tcgatgtacg tcgagcagtc ggatgtcgac gccgcgaggc tcgtcacccg
     2281 cggcgacccg cgcgtcaccc gcgtcggccg cttcatccgc aagacgagtc tcgatgaatt
     2341 gccgcagctg ttcaacgtgc ttgggggcac gctgtcgctc gtcggccctc gcccccacgc
     2401 actccaggcc aaggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgcc
     2461 acaaggtgaa gcctggtatc acgggttggg cgcagatcaa cggctggcgc ggcgagaccg
     2521 atacgccgga gaagatccag aagcgcgtcg agcacgacct ctactacatc gagaactggt
     2581 cgctggtgct cgatgtctac attctgctga agaccccgct gtcgctcctc tcggcaaaga
     2641 acgcctattg acgggcctgc tccggcacga gacctgcaga tgctaccaga gcacaacatg
     2701 taagcacgag gccgctggca cagccggtcc gcggagggac aagcgttcat gcgatcgatg
     2761 acttccagcg cgctgccggc aatgggtcta tcggacagga tcgcggccgt cgacctcacg
     2821 cgccgtcagc tcggtgcagc gggtgtcgcc ttcgcgatcg gcacgagcgc ggtcgtcttc
     2881 agcgagccgg ccccgaccga tctcgtgatg atggcgctca tcccggcgtt gccgctgctc
     2941 ggcctcgtgc gcgtcacgcc ggcactcctc gcctacctcg cgctctggct cgtgtgcggc
     3001 gccggcgccg ttgtcgccgc gaccttctcg caagacgttt cccgctcgag cgtgcactcg
     3061 gcggtctcga tctacctcta cctcgcctcg ttcgtgcttg ccggctacat agccgccgac
     3121 ccggtccggc gcaccgaact cgtgctccgc gcctacgtgg tcgctgctgg cgttgccgcg
     3181 gctgcggcgc ttgccggcta cttcgcgctt gttccttggg cctacgacct gttcacgaag
     3241 ttcggtcgag ccagcggtac cttcaaggac ccgaacgtgc tcgggccctt cctcgtgccg
     3301 gcagtcctct atctcgtgca tcgggcggta tccgagccgg cgcagaaaat ggccggcccg
     3361 ctcgcaatgg cgctggtgct cgtcgtggtc gtggtgctga gcttctcgcg cggcgcctgg
     3421 ctcaatctcg ctgtggcgct cctcgtcttc ggctacctcc acttcgtgac agcgcgctcc
     3481 gaccgccatc gcctccgtct cgtcgtgctc gcgttcacgg gtctcgtcgc catcgccgcc
     3541 atcatcgtcg tagccctcca gttcgatggc gtcggccgcc tgctcgccga gcgcgcgagc
     3601 ctgacgcaga gctacgatgt cgggccggat ggacgtttcg gcggccacga taaggcgcgc
     3661 gcgctgctgc tcgagaaccc gctcgggatc ggtgcgctcc agttcgcggc ccgcttccat
     3721 cacgaggacg tgcacaacgc ctacctcaac atgttccttt cggcgggctg gatcggcggc
     3781 ctcctgttca tcatcatcct tgtcgggacg atgctgctgg ctctccgcca tgctattggt
     3841 cgagctgagg tttcgccgct cttcctgatc gctgttgccg cgcttgccgg caatgtcgtc
     3901 gagggcttcg tgatcgacat cgatcactgg cgtcacctct atctgctgct tgccgtcgct
     3961 tggggcttca tgaccgcaag gaccggagag cgcgcatcag gctgacggat cggcggatca
     4021 cctttcgccg cgaacccctc ggaaccggat cgcggggcca cgctgccccg cgtgttgctc
     4081 agtgatcggc atgtcagaca ttagactcca cgaaactctg agatcgccgg ggcctgtgcg
     4141 ccgcctggcg ctgctgctcg tatgggtcac cgtcgcttcg ggtgccgtgg tcttcgccga
     4201 gccggcaccg gcagacttcc tgatgatggg cttgatcgtg ctgctgccgg tgatcgggct
     4261 cgtggcgatc cggcgcgagc acatcgccta tctctcgctc tggctcatcg tcgccgcctg
     4321 cggtttcgcc gcggccttca tcgccgagaa cgtcgagcgc gcgacgacgc atacggcgat
     4381 cacactctat ctcgcgatcg cctcgttcat ggtggcggcg ttcgtcgcgc gaaaccccgc
     4441 gcgccatgcg cgactgatcc tcggcgccta cacgtttgcc gccactctga cgagcctctt
     4501 cgccatcatc ggctacttcg ggctcattcc ggaggcgacg gcatggttca cgctccacca
     4561 gcgcgccagc ggcacgttca aggacccgaa cgtgttcggc ccgttcctgg tgccggcaat
     4621 catctacgcg ctgcatcgct ggatcgagcg gccggcgcgc cgggcaatgc tcgcgctcct
     4681 cgtgctaccg gtcctcatcg tcgccatgct gctgacattc tcgcgtggcg cctggctcgc
     4741 gctcgcactg tcgctcgcag ttttcggcta tgcctcattc atcacagcgg caacgaaccg
     4801 gcacaggctg aaactcatgg tgctcgcagc tttcggcgtc gcggcactcg ccggcgtcgt
     4861 tgcgagtgcg ctgcagatcg acggggtcgc aaggctcttc gatcagcgcg ccgcactcac
     4921 gcagagctac gacatcggtc cggaggggcg cttcggcgga caggacaagg cgatcgcgct
     4981 catcctcgcc catccgtttg gcatcggcgc gcgcgagttc ggcggccgca tgcacccgga
     5041 ggacccacac aacgtctacc tcagcatgct gctcaatgcg ggctggctcg gcggactcac
     5101 ctacgccctc atcattgtgc tgacgatcgc cttcttggcc cgcgcactca tgcgggcgac
     5161 tgcgacacgg ccggagctcg tcgtcgtcct tgcgtcgctc gccggcatgg cctcgctcgg
     5221 cttcttcgtc gaccaggacc actggcgcca cctttacctc ctgctcggcg tcggctgggg
     5281 cctcatgacg ggtgatcggc gtctgctccg acccgcgcgg gtgatggtgg cgaggcggct
     5341 cgtgcccgag cacctaggtc tccagatctc gcgtcgccgc gcgcggatca tcgggccgac
     5401 gctgaggccg atgcctactc tcgtcatcgc cccgacggtc acgcggcgga ccgcgcggcg
     5461 cctgcgccgg tccgcccgga tcctcggccc cgcctgaggg acgcgcgacg gttgaccggc
     5521 gcaatgttac aatataacaa tcatgcatcg tttccattgg atcgaggcat caggcatgtc
     5581 gcagagttgt cggttcccgc gcactgcaag cacgatcgca gccgccgccc tttggctagc
     5641 ggtagccgga acggcgcgcg gtgccccgcc cgaggtgatc gcgaccgtga agcccgtgca
     5701 ctcgatcgcg gccgccgtca tggacggcgt cggcgtgccc cgcctgctga tcgatggcgc
     5761 cgcctcgccg catacctacg cgctgaagcc gtccgatgcg aaggccctga acggcgcccg
     5821 cctcatcatc cgcgtcagcc cggggctcga gacgtttctc gacagcacgc tgaaatcgct
     5881 gccgaagtcg gttcgcgtcc ttagcctcga tcgcgccgag ggcatggtcc tgcttgccgt
     5941 gcgcacgggc gcgaacttcg atggcgatga ccacggtcat gggcatggcc acggcaagtc
     6001 gaaatctgca gcgatggatg cgcacatctg gctcgacccg cgcaatgcag ccgcgatcgc
     6061 gggcgcggtc gcgggcgccc tctccgacat cgatcctgcc aatgccgagg cctataggac
     6121 caacgccaag cgagccagcg acaagttcgc caacctcgac cgcgaactcg agtcactggc
     6181 ccgcccgctt gccggcaaac cgtttgtcgt cttccacgac gcctaccagt acgccgagaa
     6241 gcgctatggc ctcacggccg tgggcgcaat cacggtgagc cccgaaatcc agccgagcgc
     6301 caagcgcctg caggccctga ggcgcaccat tcagcggctg cacgcccact gcgtgttcgt
     6361 ggagccgcag ttcccgccga agctcatcga tacgatcatc gagggcagca aggcgcgccg
     6421 tggcacggtc gatccgctcg gcgtcgccat tccggcggga cccgaccact acgcgacgct
     6481 catgcgcact ctcatcgccg acttcgcggc atgcctcgcg ccgaggtcgt gagccgcatc
     6541 atgaaaatgg ccgccgctcg atcatcgagc cggcggccat ctcaggcgct gattagtccg
     6601 tcgccgtgag gcgtcagagc gcgcaaatcg tgcccttcgg gcggcagctg cctttattct
     6661 caagtgcgct ccagcagcag acctcgtcga tcttgcactt ccagttgctg gccttgcccg
     6721 agatcgggcc cttatacatg cacatcttgt tgcggtactt gggcttcgcc tcgctgtccg
     6781 agacggacat gccgaacggc acggcaaacg agagggcgac gccaagaccg agaccaagta
     6841 tccagcgctt catgcggact tcccccgtgt tttttgtggc atttacaagc gatagtttgc
     6901 ccgatttgcg cgtcgctcgc caacgcaatt ctacggaggg cgaacgagca atctcgcacc
     6961 tgcgttcagc gagtgctcag ggtgagcatc gctctgcagg ccacccgctt ggatcaggca
     7021 tcgatacgtc cgacgcccat ctcggccagc acctcgcgca cgttcgcgat gaaggcgcgc
     7081 atgtggccct cgtcgatgcg gccgacgcag ccgatccgga agctgtcggc gaccgtgagc
     7141 ttgcccggat agatgacata gccgcgctcc ttcaggcgat cgtagaaggc ctggaacttg
     7201 aacgctgcat tcgtcggcat gtggaacgtg tagatgatcg gcgcctgcag gttgtcggag
     7261 aggagcggcc ggaaacccaa cgcgcgcata ccttcgagga ggacgcgccc gttcgcgacg
     7321 tagcgccccc cgcgccccgc ctgccctcct tccgcgaaga actcctcgag tgcctggtgg
     7381 aacgagacga tgacatgaat cggcggcgtg aagcggtatt gcccggtctt cgcgaagttc
     7441 tgccactggt cgtgaaggtc gagggtgagc gacgtcgcgt tccccttggt acgcgccagc
     7501 gcgctttccg aggcgatcac gaagccgagc ccgggaatgc cctcgatgca cttgttggat
     7561 gacgcggcaa cggcatcgaa gcccgcggtg acatcgagcg gcagcgcgcc gaacgccgac
     7621 atggaatcga gcaggaaccg ccggccgtgg cgctcagcga tcgccccgat ctcgcggatc
     7681 ggattgagga tgccggaggt cgtctcgcaa tggacggcga agacgtgcgt gaggcgcttg
     7741 tccgcggcga gcagcttttc gacggcggcg aggtcaggcg gacaatcctc cgccgtctcg
     7801 tggacgacgg catgccgtcg cgcgatgtcg agaatacgct tggcgcgctg cccgtaggca
     7861 ccgttgacga ggacgaggca gccgccatcc gccggaacga aggaggtcag catcgcctcg
     7921 acggcatagg tccccgagcc ctgcaccggc accgtgacaa agccgtgctc cttaccgccg
     7981 acgatgtcga cgaggcgctc gagcacggcc ttgttgatcg cgaggaacgc ggcatcgcgc
     8041 gaaccccagt cgtgcaccat cacctccttc acgcgcttcg aggtggtgag cggaccgggt
     8101 gtcagcagca gcggatcgcc cgtttcggag acacctcgtt gtgccttgcc ctgtgccgct
     8161 gccggccgcg tgctcgaagt gctcacgatc gtgctcctga tcctatggtg cgaaggtcgt
     8221 ccggagtctc aagtcgtctt caggccgcgc ttggcgagca tggcgtccgg cgtcggcagg
     8281 cggccacgga acttcacgta ggcctcctcg ggcgggcggc ggttgccggc tgagtatatg
     8341 tggtccttga gcttcttggc cgtcttggcg tcgaatacat cgcccgcctc ctcgaaggcc
     8401 gagaacgcgt cggcatcgag cacctcggac cacatgtagc tgtagtagcc ggaggcatag
     8461 ccgcccgaga agatgtgctg gaagtggggc agccggtggc gcatcgtcac ctcggcgggc
     8521 atgccgatgc gggcgaacaa ctcggcctcg aagcggccga ggtccgccgg caactcgtcc
     8581 ggcttgcact tgtggagctc catgtcggcc attgccgagg cgatgaactc ggtcgtcgag
     8641 aaaccctggt tgaaggcgcg cgcggccttc agtttggcga gcagcttcgc cggcatgggc
     8701 tgccccgtcg tgacgtgcac ggcatgcttc tcgagcacct cgcgcgtgcc gagccaatgc
     8761 tcgtagagct gggatggcaa ctcgacgaaa tcgcgcgcga cggccgtgcc ggcaatcgag
     8821 ggataggtga catccgagag gaggccgtgc agcgcatggc cgaactcgtg gaagagcgtg
     8881 cgcgcatcgt cgtaggagag gagcgccggc tgcccctcgc ccgggggcgc gcaattcagc
     8941 acgttgagaa tgatcgggcg tacctcgccg tcgagcttcc actgcgagcg gatcgcactc
     9001 ttccaggcgc ccgaccgctt cgtcggccgc gcgaagtagt cgcccatgaa gacgccgatg
     9061 tggcggccgc gcttgtcgcg cacctcgtag acgcgcacat ccgggtggta gcgcggtgca
     9121 tcgggcagct cgcggaactt caggccgaac aggcggcccg ccacgtcgaa cgcggcctcg
     9181 atcatgcgct cgagctggag gtagggcttc agctcgctct cgtcgaagtc gtatcgcgcc
     9241 ttgcgcacct tctcggccca gtagcgccag tcggcggcgc gcaggggtgc attgctgccc
     9301 tccttgcgcg cctcggccag caatgcctca cgctcctcgc cgaccttgcg cttggcggcc
     9361 gtccagacct cgccgagcag cccatcgacg gactctggcg tcttggccat ggtgtcgtcg
     9421 agactgaagg ctgcatgcgt cggatagccg agcagcgcgg cccgctccgc ccggagcttc
     9481 agcgtttcgg taataatctc gcgattgtcg ctggcgccac ccatctcgcc gcgcctggac
     9541 cacgcgtcga aggctttggc gcggagatcc gcgcgggcgg agaactggag gaacgtctcg
     9601 atgctcgacc gcgccagtgt cacggcgtgc tttccttcat ggccgaggtc tgcggcgagc
     9661 cgcttggcgg ccgcccgcgc gaaatcgggg aggccggcaa gatcgctctc accatcgagg
     9721 atcagcttga aggcctgctc gtcggccagc acgttctggg cgaaggacgt cgcgagcgtg
     9781 gcgagccgct cgttgatcgc ggcgacgcgc ttcttcttcg caggcgggag ggcggcgccg
     9841 gcgcggacga gattgcgatg cgtgcgctcg agcacgcggt gctgctcgtc gtcgagcttc
     9901 agccgcgctc ggcgcgagtg gaggtcatcg atacgctgaa agagcttggc atcgagcatg
     9961 atacggttgc gatgctgcgc catgagcgga gcgagttcgc gctcgatcgc gcgcagctcc
    10021 ggcgtcgaga cggttccggt aaggttgtag aagatgccgc cgacgcgcga caggagccgg
    10081 ccggagcgtt cgagcgcgtc gatcgtattg gcgatcgttg gccgggactc ggacttcgtg
    10141 atcgccgcga tctccttcag gtgctcacgc atggcggccg cgaatgcggg cttgtagttc
    10201 tccaggcgga cggcctcgaa tggcggcatc gcgaagggcg ctgaccaggc accgaggagt
    10261 gggttgtccg cagcgcgcgg agacgtcgcc gctttgccgg ctgatcggtc gctggacttg
    10321 cgcggcattg ggcgctccgc ttggctatcg gatttcggga ggtgggctgg ctcgccaccc
    10381 ggttacgccg tctcgggggc ggcggcaaca ccgcgctcga gcttcagccg cgtctcgttg
    10441 agctcgcccg cggcctcgag gatcggatag gcgaccgagg tcagatgcga gttgattcgt
    10501 ttgaggtcgc gcacgatgtc gagatgtatg gagctcgtct cgatcgattc ctgccggccg
    10561 gccttcaggc gcgcgaaatg ccggttggcg gcatcgaact cgctcgcgcg catatcggtc
    10621 ttggcgagca ggaggcggcg ggccagcgcc acgtcacgca gagcgaacac gttgagggcg
    10681 agccgcatgt tctcgagcac ctgcgcgtgg aacgtcttca gctcggcgag accctccgag
    10741 gaaaaggcgt agcccttctt cgccttcttc tgggcgagct ccatcaggtt cttgtcgatg
    10801 atgtcgccga tgtgctcgag gttggtcgtg aaggtgagga tctcgacgta gcggcggctt
    10861 tcgccctcgt ccatgtcggt ctttgaggcg cggatcatgt agagcttgat ggcctcgtgg
    10921 agcgcatcga cgtggtcgtc cgtacgctcg atctccttga tgagtttcgt atcgttcgtc
    10981 tcgatcgcgg tcagcgagcg gcgcagcatg tcgtgcacga tgttgccgac ggccagtgtt
    11041 tcgcggagcg cacagcccat cgcctcggag ggactgtcga gcgcactcgg gtcgagatgc
    11101 tgcggcaggc gcggatcggg cgcaagcttg gggtcgggta gcagccgctc gaccccctca
    11161 gcgagcggcc tcaacagcgg caggaacgcc gcagcgaccg caacgttgaa caggcaatgg
    11221 aaattgagaa cgagttgcga gggtacggct gaagcgtact cgagcaacgg cacgagcctt
    11281 tggacgaacg gcaacatcac aacggcgatc acgacccggg cgatgaggtt gccgacaggc
    11341 acgcggcgcg ccgcggtcgg tgcgccggtg agcgcggtca acggtgcgat agccccaccc
    11401 acattggcgc cgacgacgag agcaagcgcg agcgcaggct tgatcacgcc gctcgccgcg
    11461 agcgacatga cgaagagaat gatggcgagg ctcgaatgcg cgagccaggt caccaaggcg
    11521 gcaacgacgg ccgcgacgat cagctccgag ccgagagccg aaagcagcac ggccatcgcc
    11581 tggctctcgc ggatggcgcc ggagacgttg gcgatgacgc cgagggccag cagcatgagg
    11641 ccgagcccga tcaggatgcg gccgacgctc ttcgcccgtt cgccgcgtgc cgcattgaag
    11701 gcaacgacgc cgccgaacat gaggacgggc cagagccagc ggatatcgag cgagaagagc
    11761 tgggcgacca gcgtcgaccc cacgtccgcg ccgagcatca cggcgagcgc gagtggcagc
    11821 gtgatgagcc cgcggccggc gaacgaggag agcagcagcg acgtcgccgt ggcactctgc
    11881 agcaccgcag tcacgcccaa tccggcgaga aaagcggtca tgcgattgcg cgaggcttcc
    11941 ccgagcagcg cccgaagcgg ccccgaaaag gcgcgggtga gccccgtccg caccatgcgc
    12001 acaccccaaa gcagcagtgc gacggcaccg agcagcgtca ggagcacctc gctgcctccc
    12061 atggtcttcg gctccttgct cgttcggtga ctattggacc cgcacgagag cggggtccgg
    12121 ccgactgtca caatcgcccc cgacggggcg aagcgcaact gacaacacca cttcggcaga
    12181 agatggatcg caggatctgg ttgagaacgg cgcagcttcg cttgagcgcc ttgacatccc
    12241 aattggcggc atcgacgtga cagatctgga acgtgtcgct aatctcggcg aattcatcat
    12301 taccggaggg gtctgatgcg gctcgtagca gcgctgttct tcctgctgag cgtcctcgct
    12361 gccgagccgg cgcacgcaca gggcaagcgc gtcgccctcg ttatcgggaa cgaggcctat
    12421 gcgcatgcct ccgcgctgaa gaaccccgtc cgggatgcgg cagcggtcgc cgcggccttg
    12481 aagcggatcg gcttcgagct agtggaggtt cagcgtaacc tcggcgtcga tgcgctgagg
    12541 cgcgccctcc tcgccttcga ggggcatgcc accggcgccg atatcgcgct cgtcttcttc
    12601 gcgggccacg gcatcgaggt ggacggcgtc aactatctgg ttccgacgga cgcacgcctt
    12661 acccgcgcca atggcgtcga gttggaggcg gtggccctgc cgacggtcac cgccgcgatc
    12721 tccggcgcgc ggaagctgcg cctcgtgatc ctcgattcct gtcgcaacaa tccgttccag
    12781 ccgcggctcg cggcggatag cggccgcaag cgctcgatcg gccgtggact ggcacgcgtc
    12841 gagcccggcg acaacgagct catcgccttc gctgcggctg ccggcaccac cgcggacgac
    12901 ggcgaggcgg gaaacagccc gttcacgacg gcactgttga aacatctgga acggccgggc
    12961 ctcgaggtgc agcagctctt ccgcaacgtg cgtgacgaag tgatcgcgtc gacggcgcga
    13021 ggccaggtgc cacacgtcta cggcacgctc ggcgcggagc ccgtgttctt ccgggccgcg
    13081 ttggcaagcg tcgacaccgt caaggccaac gaagccgaga tcgcgcgccg cgtcgcggcc
    13141 gagcgcgccc ggctcgaggc cgagatggcc gcgcggctcg aggcggaaaa gaggcggctg
    13201 gcggcggcga cgcc
//
LOCUS       DAORNH010000105        13177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3558_length_13177_cov_2.720317, whole genome shotgun sequence.
ACCESSION   DAORNH010000105 DAORNH010000000
VERSION     DAORNH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13177
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3558_length_13177_cov_2.720317"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1255)
                     /locus_tag="PK264_12025"
     CDS             complement(80..1255)
                     /locus_tag="PK264_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HRD76649.1"
                     /translation="MRTRLTESLTLPSDGTSGTLVGRVWRPEVGGPSVVAVRADGVVD
                     ISAHAATMRDLCERADAVEIARSGKGELLGSFASILANTSEQHRDDAKPWLLAPTDLQ
                     AIKAAGVTFARSMVERVIEEQAKGAPEKAEAIRAEVERQLGGDLRRLKPGSPQALALK
                     AALIKAGAWSQYLEVGIGEDAEIFTKAQPMSAVGHGMEAGLHPRSTWNNPEPEVVLVC
                     SSKGEIVGATLGNDVNLRDFEGRSALLLSKAKDNNASAAVGPLIRLFDGTFTLDTVRR
                     IEVSLTVDGEDGFRMTGKSSMSEIARDPADLVGELFGKTHMYPDGAVLYLGTMFAPVE
                     DRGAKGMGFTHKVGDTVTIFASELGSLTNRMVHADKAAPWSFGTADLMRNLARRRLI"
     gene            complement(1286..2563)
                     /locus_tag="PK264_12030"
     CDS             complement(1286..2563)
                     /locus_tag="PK264_12030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD76650.1"
                     /translation="MSKTVIEDGPPAKAASPPARAAVSLIVMLTLTTLLSQFYRSSST
                     VIAPELIRDLRLTPEMLGFANACFFLALLAVQIPVGLLFDRIGARLTVVGLSVAAVGG
                     ALLHAWVESGAGLAMARFLLGIGHGASFMATIFLISRWWPRERWSQTISWVFASSMLG
                     VMLAGTPLAIASEWIGWRGAFLVMSLLSLTTGILFVIAVSDDPPGRAVPLRQHERFST
                     ALYGFIEVMQLPGLWRILGLQFVAYAVMASIMGLWAGPYLNDVHGFTVRERGDTLIAM
                     ALAQTIGTLAFGPLDRLFNTRKWVAVAGALATIATLLALAASPRPPTWFAVALMVVLC
                     AVSSYGVMVVTHARSLYPDHLAGRGTTTANIAQLIGCALIPLGTGFIPSLFPVTAAGY
                     APAAYQLIFAAIAGSLALGLVIYLTAKDVRPKS"
     gene            complement(2621..3091)
                     /locus_tag="PK264_12035"
     CDS             complement(2621..3091)
                     /locus_tag="PK264_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76651.1"
                     /translation="MMNGSILRTSLATVAAIVYAFLGVSGHAAASDSPRWLTVAEVRG
                     ALLGRPAAGIYPDGTPWAEIITANGRADYSERGERRGGSWSFNLEGELCFRYDDGRGG
                     GCFRYVQISSNCYEHFYQVFGPKTDSTGPDDRFLTNGRLWRTDEPQTCDEKPAV"
     gene            3277..3747
                     /locus_tag="PK264_12040"
     CDS             3277..3747
                     /locus_tag="PK264_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76652.1"
                     /translation="MNNVNNDTGRTAFCGPYVLAAITGFPISRIEEEIHKFRETPVER
                     RPIIRGTNAGEVSAALAHFGYRMDLKEGYMHLERKARPTLWSWMQKPRSAWSHYIIAI
                     HKGKEGHWVLIKGVKLCDTFSAGKWQFVCDGPHKGARIMEVFEVKRDHEHGLAR"
     gene            3883..4071
                     /gene="rpmF"
                     /locus_tag="PK264_12045"
     CDS             3883..4071
                     /gene="rpmF"
                     /locus_tag="PK264_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRD76653.1"
                     /translation="MAVPKKKTSPSRKGMRQSADALTAPTYIADAKTGDLRRPHHVDP
                     KTGLYRGRQILPAPKEEE"
     gene            4239..4658
                     /locus_tag="PK264_12050"
     CDS             4239..4658
                     /locus_tag="PK264_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76654.1"
                     /translation="MMSLRSLPLMIIPFIIYNILAYTFSGTEADAVFRDRLFDLPLGG
                     GRKWTFSKGDLVMLLVMLCLFFELIKSTWTGSSSLIDHGLSMLVFIFCLIEFILVPRA
                     ATSVFFFIMVAALIDVIAGYTIGIRVARRDVSFGGDA"
     gene            4835..6592
                     /locus_tag="PK264_12055"
     CDS             4835..6592
                     /locus_tag="PK264_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HRD76655.1"
                     /translation="MSGPREARIADHAIDTPVASPLDDGSPEAGIDLLGLLSSVQETA
                     YLWDIRSDRIDWESNALEVLGLHSAGQVGTGADFQFLIAPEHVKRRQDAILKLQPVEG
                     ASGAPYRVQYRFTPAGRRSDAALWLEDHGRCWFDQARQPVRARGVIRVINERYWEEQR
                     LLYRSDHDELTGQLNRIRLTEALGAVVTRAERTKHTCAFLMIAVNNLAVINETFGFDV
                     GDEVIASVAKTVRKLLRGGDSIGRYSSNKFGIILNDCSPVAMRTAADRFMKAVRELPI
                     KTAATQLSATVSVGGVIVPDQAQTVHQALSHALQALDLAKARRSDSFMAFEPSAVKET
                     ARLRNITIADEVIAAIDENRMRLALQPIVDTATREPALYECLLRMEKPNGLVVSAGEF
                     IVVAEQLGLSRLIDRRTLELAVELLKKHPTLALSLNVSSLTSSDHDWLVALHRLTGGR
                     RQLTSRLTIEITETSAIHDIDQTVAFVDTLKELGCRVAIDDFGAGYTSFKNLKHLKVD
                     MVKIDGSFVKNLTEDPTNRVFIRALVDIARTFGMATVAEWVADEPTVEALKDAGITHL
                     QGFLFGQPLDPSELYAARR"
     assembly_gap    6678..6691
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6825..7445
                     /locus_tag="PK264_12060"
     CDS             6825..7445
                     /locus_tag="PK264_12060"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRD76656.1"
                     /translation="MAVWAVLATAALAYLAVFVHRPDLIHRFAVRPQRDGVELQRLVE
                     RMASENAALRGEMKGLKADLDDLRSVVAERQARDQAIADRMAAMSPPPSGASAEKGVT
                     TGAIGPAVLAVSPAPAAPAAEPKALQLTTASSLEALRLAWSLLTDQNKALFRGLEPRY
                     VEVEGGGSYQLLAGPLKADADALKACNTLKQRRLPCSVVPFRGAAL"
     gene            7581..7979
                     /locus_tag="PK264_12065"
     CDS             7581..7979
                     /locus_tag="PK264_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HRD76657.1"
                     /translation="MGVFSEIFSWWGGNTWSNRIYTAFRGRLVGVDEQGNRYYEQSRG
                     VGPLGVPRRWVIYKDLAEASKITPDWHGWMHHTVDTPPTKESYTPRPWQKPHRMNMTG
                     TSEAYRPEGSILAKGVRPKATGDYKPWRPE"
     gene            7964..8974
                     /locus_tag="PK264_12070"
     CDS             7964..8974
                     /locus_tag="PK264_12070"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRD76658.1"
                     /translation="MAARIGASVMPRPSLGTAAKLLTGVALLAVLVWQVDIAKVGALL
                     RRTDPLLFIGATCAAGIGLAAQALRWSIVARELTPVLSLRDALIGVYEGQFVNQIMPA
                     MIGGDAVRALRAYDAGATAGRAILGVLLDRAFGLLFIALIVIATFALSAAPVVAAPVF
                     QFITIAAGLVVAGALALAIVGPLVATLSLPHWLAALASVIAGFATIARRPIAMLQLAA
                     AMLVAAAGLAAGIALSARAIGVTLSPVDAIVVLTGIVIAGAIPLSVGGWGLREGATVL
                     LLKAAGVEAAAAVSLAILFGLALTVLGIAGGAVWLTSGYRRVDRATGLGELKRAATGA
                     PP"
     gene            8971..10440
                     /locus_tag="PK264_12075"
     CDS             8971..10440
                     /locus_tag="PK264_12075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD76659.1"
                     /translation="MITVPGEGTRAGPELTPRKQAFRDNADRMAPERRKWVQRNRGYY
                     GDDRRYMRFLIPRGARVLDLGCGNGDLLAALHPSRGLGIDFSPRMIEEARRSHPNLEF
                     MLADAENASEMAAIEGPFDFIILSDTIGTFEDIEEALRLLHRLCTPSTRIVIAYYSPS
                     WDPLLRLGTMLGKRMPQPKPNLISMVDFLNILDLAEFEPIRIERKQIVPLWLFGLGRL
                     INRFIQPLPGIRSFGLRMYVVARSKRAAPKPAPSTSVVIPCRNEKGNIERAVRELPRF
                     GRHQEIVFVEGNSSDGTYEECLRVKEAYQGQWDIKVLKQDGRGKGDAVRKGFTHASGD
                     ILMILDADLTMPAGALPKFYDAIATGKGEFINGTRLIYPMEDEAMRFLNHAANRTFAA
                     LFSYLLNQRFTDTLCGTKVLWRTDYDQIVANRAYFGDFDPFGDFDLIFGAAKRNLRIV
                     EVPIHYKARTYGETQISRFRDGWLLLRMVVFAFKKLKAI"
     gene            complement(10865..12356)
                     /locus_tag="PK264_12080"
                     /pseudo
     CDS             complement(10865..12356)
                     /locus_tag="PK264_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(12454..>12783)
                     /locus_tag="PK264_12085"
     CDS             complement(12454..>12783)
                     /locus_tag="PK264_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRD76660.1"
                     /translation="SDAPTHYEAPTGQRHPIEYEGPGAPILAIRVQQLFGLTVHPAIA
                     RGRLPLTLHLLSPAGRPIQITRDLPGFWKGSWAAVRAEMRGRYPKHPWPEDPATAPPT
                     TRAKPRS"
     assembly_gap    12784..12925
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12926..>13177)
                     /locus_tag="PK264_12090"
     CDS             complement(<12926..>13177)
                     /locus_tag="PK264_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76661.1"
                     /translation="SERDEVFFDAEAKAVRARRTRSLDAITLESTPAAVAIDAASALA
                     LAKGAATLGIDKLPWSRAQRQLRDRVGFVAAAEPRLGLPD"
BASE COUNT         2189 a         4330 c         4252 g         2250 t
ORIGIN      
        1 gaggcgcgca gctccacccc cctcggcatc gtcgtcgttc cccttctggc cgtggcagtg
       61 acgtcgccgc atctgcagct cagatcagcc gccgccgcgc cagattgcgc atgaggtcag
      121 cggtaccgaa gctccagggc gcggccttgt cggcgtgcac catgcgattg gtcagtgagc
      181 cgagctcgga tgcgaagatc gtcaccgtat cgccgacctt gtgcgtgaag cccatcccct
      241 tggcgccgcg atcctcgacg ggcgcaaaca tcgtgccgag gtagagcacg gcgccgtcag
      301 ggtacatgtg cgtcttgccg aagagttcgc cgaccagatc ggcgggatcg cgcgcgatct
      361 ccgacatcga cgacttgccg gtcatgcgga agccgtcctc gccgtcgacc gtgaggctca
      421 cctcgatgcg gcgcaccgta tcgagcgtga aggtgccgtc gaagaggcgg atcagcgggc
      481 cgacggcggc gctcgcgttg ttgtccttgg ccttcgagag cagcagcgcc gaacggccct
      541 cgaaatcgcg gagattgacg tcgttgccga gcgtggcgcc gacgatctct cccttcgagg
      601 agcagacgag caccacctcg ggctcaggat tgttccaggt ggatcgagga tgcagtccgg
      661 cctccatgcc atgcccgacg gccgacatcg gctgcgcctt cgtaaatatc tcggcgtcct
      721 cgccgatgcc gacctcgagg tactggctcc aggcgccagc cttgatgagt gcggccttga
      781 gtgcgagcgc ctgcggcgag ccgggcttca ggcggcggag gtcgccgccg agctggcgct
      841 cgacctcggc gcgaattgcc tcggccttct cgggggcacc cttcgcctgt tcctcgatca
      901 cgcgctcgac catggagcgt gcgaaggtga cgccggccgc tttgatcgcc tgcaggtcag
      961 tgggcgcgag cagccacggc ttggcgtcat cacggtgctg ttcgctcgtg ttggcaagga
     1021 tcgatgcgaa ggagccgagc aactcgccct tgccggatcg tgcgatctcg acagcatctg
     1081 cgcgttcgca cagatcccgc atggtcgccg cgtgcgcgga gatgtcaacg acgccatcgg
     1141 cccggacggc gaccacggac gggcctccga cctccggccg ccagacacgc ccgacgaggg
     1201 tgccgctcgt gccgtcgctc ggcaaggtca ggctttcggt caatcgcgtg cgcatgctgt
     1261 cctccctcat ttggcgggac gttgctcagc tcttcggccg gacgtccttg gcagtcagat
     1321 agatgacgag gccgagcgcg agcgagcccg cgatcgccgc aaatatgagc tggtaggccg
     1381 ccggagcgta gcctgcggcg gtgaccggga acaggctcgg gatgaacccg gtgccgagcg
     1441 gaatgagcgc gcagccgatc agctgcgcga tattggccgt cgtagtgccg cggccggcaa
     1501 ggtggtccgg atagaggctg cgggcgtgcg ttaccaccat gacgccgtac gaggagaccg
     1561 cgcacagcac gaccataagc gcgacggcga accaggttgg tggacggggg gacgcggcga
     1621 gcgcgagcag cgtcgcgatc gtagccaggg cgccggcgac ggcgacccat ttccgggtgt
     1681 tgaacaggcg atcgagcggg ccgaacgcga gcgtgccgat ggtctgcgca agggccatgg
     1741 cgatcagtgt gtcgccgcgt tcgcgcactg tgaagccgtg cacgtcgttg agatagggac
     1801 ccgcccacag ccccatgatc gaggccatca cggcatacgc gacgaactgc aagccgagga
     1861 tgcgccagag gcccggcaac tgcatcacct cgatgaagcc atagagcgcc gtcgaaaagc
     1921 gctcatgctg ccgcagcggt actgcccggc cgggcggatc gtcgctcacg gcgatcacga
     1981 acaggatgcc ggttgtgagc gacagaaggc tcatgacgag gaacgcgccg cgccagccga
     2041 tccactcgct ggcgatggcg agcggcgttc cggctagcat cacgccgagc atgctcgacg
     2101 cgaacaccca gctgatggtc tggctccagc gctcgcgagg ccaccagcgc gagatgagga
     2161 agatcgtcgc catgaagctc gcaccatggc cgatgccgag caggaagcgg gccatggcga
     2221 ggccggcgcc actctcgacc catgcatgga gcagcgcgcc gccgaccgct gcgacgctga
     2281 gaccgaccac ggtgagcctg gcgccgatgc ggtcgaacag gaggccgacg gggatctgca
     2341 ccgccaaaag tgcgaggaaa aagcaggcat tcgcgaatcc gagcatctcg ggagtgagtc
     2401 tgaggtcgcg gatcagctcg ggcgcgatca ccgtgctcga cgaccggtag aactgcgaga
     2461 gcagcgttgt cagtgtgagc atcacgatca gggataccgc cgcgcgcgcc ggaggactcg
     2521 cggccttagc cggcggcccg tcctcgatca cggtcttgct caccccggac ctccgcctgc
     2581 ctcgacgccg gcagcgacgg cctctgccgt cttacgcggc tcagacagcc ggcttctcgt
     2641 cgcaagtctg cggctcatcg gtccgccaga ggcgaccgtt ggtgagaaag cggtcatcgg
     2701 ggcctgtgct gtcggtcttc gggccgaaca cctgatagaa gtgctcatag cagttgctgc
     2761 tgatctggac gtagcgaaaa cagccgccac cccggccgtc gtcatagcgg aagcagagct
     2821 cgccctcgag gttgaagctc catgagccgc cgcgccgctc gccgcgttca ctgtagtcgg
     2881 cccggccgtt ggcggtgatg atctctgccc atggcgtgcc atcagggtag atgccggcag
     2941 ctggtcggcc gagcagggcg cctcggacct cggcgaccgt gagccagcgc gggctgtcgg
     3001 aggcggcggc gtgcccactc acaccaagaa acgcgtagac gattgccgcc accgtcgcca
     3061 gagacgtgcg tagaattgat ccgttcatca tggtggctcc gcaacaatcc gaaacaaatg
     3121 gcaagtgttt gttaacccca cggcgttaga tcggtctccc ccgctctctg gtcaagaggt
     3181 gctgccatgc tgccagagct cgacgccccc gtgtttgatg agacgaacat tgcgaacgtc
     3241 gcttcaacga tggaggcccg acgaacgacg tcggccttga acaatgtcaa caacgacacc
     3301 ggccgaacgg cgttctgcgg cccttacgtg ctcgcggcca tcacgggctt cccgatcagc
     3361 cggatcgagg aggagatcca taaattccgc gagacgccgg tcgagcgacg cccgatcatc
     3421 cgcggcacca atgccggcga agtgagcgca gcgctcgctc atttcggata tcgcatggac
     3481 ctgaaggaag ggtatatgca cctcgagcgc aaggcgcgtc cgacgctctg gtcgtggatg
     3541 cagaagccgc gctcggcctg gtcgcactac atcatcgcga tccacaaggg caaggaaggc
     3601 cactgggtgc tgatcaaggg cgtgaagctc tgcgacacgt tctccgcagg taagtggcag
     3661 tttgtctgcg atggccccca caagggcgcc aggatcatgg aagtcttcga ggtgaagcgc
     3721 gatcacgagc atggcctcgc acgctagtcg ggcgatggcg agcgcgtccg gattgccgct
     3781 ggtcattgcc atggattgtg agtataaggc gggcgatttc gtgctccgcc tgccgcgccg
     3841 acgcgcggcg acggggccgt tgcgcgcctc gaggagcttg ttatggccgt acccaagaag
     3901 aagaccagcc cgtcacgtaa gggcatgcgc cagtcggcag atgcgctgac agcgccgacc
     3961 tatatcgccg acgccaagac cggtgatctg cgccgccccc accacgtcga tccgaagacc
     4021 gggctctacc gcgggcgtca gattctcccg gcaccgaaag aggaagagtg accctttgct
     4081 ccaactagcg cgcgtgccca ttccgggatg atcacgtgca gcagggggag cgcgcctcgg
     4141 gccaaactgc cgccacgcca tggcgacgtc gtagcatgct gcctgccgac aactccatag
     4201 tccaggacgc aacaccaaga gcgaacggag atcactcaat gatgtcgctt cgatcactgc
     4261 cgctgatgat catcccgttc atcatctaca atatcctggc ctatacgttc tcgggcaccg
     4321 aggccgatgc ggtgttccgc gatcgcctgt tcgatctgcc gctcggcggt ggtcgcaagt
     4381 ggacgttctc caagggcgat ctggtcatgc tcctggtgat gctgtgcctg ttcttcgagc
     4441 tcatcaagtc gacctggacc ggctcgtctt cgctgatcga ccacggtctc tcgatgctgg
     4501 tgttcatttt ctgcttgatc gagttcatcc tggtgccgcg agccgccacg tccgtgttct
     4561 ttttcatcat ggtcgcggcg ttgatcgacg tgatcgccgg atacacgatc ggcattcgcg
     4621 tcgcccggcg tgacgtgtcg ttcggcggcg acgcctgatc gttcctcgga tctgcagcga
     4681 atacgttcga tggctggccc agcgccggcc atcgcgcgtt ttggggcgac caatcggcaa
     4741 ccgacggagg atatcgggac cgcaattcag cgattcttag gatttgtggc catactttcg
     4801 gggttcatgc ggcaccgatc agggaccatc gattgtgagc ggacctcgcg aagctcgaat
     4861 tgccgatcac gcgatcgata cgccggtcgc ctcgccgctc gacgacggca gtcccgaggc
     4921 cggcatcgac ctcctcgggt tgctctcctc ggtgcaagag acggcctatc tctgggacat
     4981 ccggtcggac cgcatcgact gggagagcaa cgccctcgag gtgctgggcc tccactcggc
     5041 tggccaggtc ggcaccggcg ccgacttcca gttcctgatc gcgcccgagc acgtcaagcg
     5101 acgccaggat gcaatcctga agctccagcc ggtcgaaggg gcgagcggcg ctccctatcg
     5161 cgtgcagtat cgcttcactc ccgccgggcg acgcagcgat gccgccctct ggctcgagga
     5221 ccatggccgc tgctggttcg atcaggcccg ccagccggtc cgcgcgcggg gcgtcattcg
     5281 cgtcatcaat gagcgctatt gggaggagca gcgcctcctc tatcgctcgg accatgacga
     5341 actgaccggc cagctcaacc gcattcgcct gaccgaagct ctcggtgccg tggtgacgcg
     5401 cgccgagcgc acgaagcata cctgcgcctt cctgatgatt gccgtaaaca atctggccgt
     5461 catcaacgag accttcggct tcgacgttgg cgacgaggtg atcgcctcgg tcgccaagac
     5521 cgtgcgcaag ctgctgcgcg gcggcgacag catcgggcgc tactcgtcga acaaatttgg
     5581 catcattctg aatgactgca gccccgttgc catgcggacc gccgccgacc gcttcatgaa
     5641 ggcggtccgc gagctgccga tcaagacggc cgccacccaa ctttccgcga ccgtttccgt
     5701 cggcggcgtc atcgtgcccg accaggcgca gaccgtgcac caagccttga gccacgcgct
     5761 gcaggcgctt gacctcgcga aggccaggcg ctcagacagt ttcatggcct tcgagccgag
     5821 cgccgtgaag gagacggcgc ggctccgcaa catcactatc gccgacgagg tgatcgcggc
     5881 aatcgacgag aaccgcatgc ggctcgcgct gcagccgatc gtcgacacgg cgacgcgcga
     5941 gcccgcgctc tacgagtgcc tgttgcgcat ggagaagccg aacggcctcg tcgtctcggc
     6001 cggcgagttc atcgtcgtcg ccgagcagct cggactctcc cgcctcatcg acagacgcac
     6061 gctcgagctc gccgtcgagc tcctgaagaa gcatccgacg ctggcattgt cactcaacgt
     6121 gtcgagcctc acctcgagcg accacgactg gctcgtcgcc ctgcatcgtc tcaccggcgg
     6181 gcgacggcag ctgacgagcc gtctcaccat cgagatcacc gagacctcgg cgatccacga
     6241 catcgaccag accgtggcct tcgttgatac cttgaaggag ctcggctgcc gcgtcgctat
     6301 cgacgacttc ggcgcggggt acacctcgtt caagaacctg aagcacctca aggtcgacat
     6361 ggtgaagatc gacggctcgt tcgtgaagaa cctgaccgag gatccgacca acagggtgtt
     6421 catccgcgcg ctcgtcgata tcgccaggac cttcggcatg gcgacggtcg ccgagtgggt
     6481 cgccgacgag ccgacggtcg aggcgctgaa ggatgccggc atcacgcatc tccaaggctt
     6541 cctgttcggt cagccgctag atccttcaga gctgtacgca gcacgccgct gagctcgctc
     6601 acgtccacag ctggatccct gccgtgcatt gacggtcttc cgcgccttcg ggtctgatgc
     6661 ctcgcgtggc cagtgtcnnn nnnnnnnnnn ngattgactt ggccctccgc tccgccgtgc
     6721 cggcctcggt tgccggcaac gggagagcgg ccggaccaga acggacaagg ggcatcgcga
     6781 tgtcgcaatc gtcgggaact gtgcgaccat cccgatcgct ctacatggca gtatgggcgg
     6841 tgctcgcgac agcggcgctt gcttatctcg ccgtattcgt ccaccggccg gacctcatcc
     6901 accgcttcgc cgtgcgccct cagcgcgatg gcgtggagct gcagcgcctc gttgagcgca
     6961 tggcgtccga gaacgcagcg ctccgaggcg agatgaaggg cctcaaggct gatctcgacg
     7021 acctgcgcag cgtcgtcgcc gagagacagg cccgcgacca ggctatcgcc gaccgcatgg
     7081 ccgcgatgtc gccgccgccc agcggcgcgt cggccgagaa gggcgtaacg acgggcgcga
     7141 tcgggcccgc ggtgctcgcc gtcagtcccg cccccgctgc tccagctgcc gagccgaagg
     7201 cactccaact gacgacggcc tcgagtctcg aagctttgcg cctcgcctgg agcctgctca
     7261 cggaccagaa taaggcgctc ttccgcgggt tggagccgcg ctatgtcgag gtcgagggcg
     7321 gcggcagcta ccagctgctc gccgggccgc tcaaggccga cgccgatgct ctcaaggcct
     7381 gcaacaccct gaaacaacgg cgcctcccct gcagcgtcgt tccattccga ggcgcggctc
     7441 tctagggccg gacgccgccg gagcggcgag gccatgtgtc aatcgtggtg tggacagtcg
     7501 actgcccgag tgcgatatcg cgaatgataa tggacgagag acgacgcgcg acggcgtctc
     7561 agtaacggcg ccgaggcatc atgggtgtgt tcagcgagat cttcagctgg tggggtggca
     7621 acacctggtc gaaccgcatc tacaccgcat tccgaggccg ccttgtcggc gtcgatgagc
     7681 agggcaatcg atactacgag cagtcgcggg gtgtcgggcc gctcggcgtg ccacggcgct
     7741 gggtgatcta caaggacctc gccgaggcgt ccaagatcac gcccgactgg cacggctgga
     7801 tgcaccatac ggtcgacacg ccgccaacca aggaatcata caccccgcgt ccctggcaga
     7861 agccgcatcg catgaacatg accgggacca gcgaggcgta cagaccggag gggtcgatcc
     7921 tcgcgaaggg tgtccggccc aaggcgacag gcgactacaa gccatggcgg cccgaatagg
     7981 cgcctcggtg atgccaagac cgtcgctcgg cacggccgcg aaactgctga ccggcgtcgc
     8041 gcttctggca gtcctcgtct ggcaggtcga tatcgccaag gtcggggcgc tgctgcgccg
     8101 gacagacccg ctactgttca tcggcgcaac gtgcgcggcc ggcatcggcc tcgccgcgca
     8161 ggcactgcgc tggtcgatcg tcgcacgcga gctgaccccg gtgctgagcc tgcgtgacgc
     8221 gctcatcggt gtctacgaag gacagttcgt caaccagatc atgccagcga tgatcggcgg
     8281 cgacgccgtc agggccttgc gcgcctacga tgccggggcg acagcagggc gtgcgatcct
     8341 cggcgtgctg ctcgatcgcg ctttcggcct cctcttcatc gcgctcatcg tgatcgccac
     8401 cttcgctctg tcggcggcgc ccgttgtcgc agcgccggtc ttccaattca tcaccatcgc
     8461 cgcgggcctc gttgtcgccg gcgcgcttgc gctcgcaatc gtcggaccac tcgtcgccac
     8521 gctgtcgctg ccgcactggc ttgccgcact cgcaagcgtg atcgccggat tcgcgacgat
     8581 cgcgcggcgg cccatcgcca tgctgcaact cgctgccgcc atgctggtcg cagcagctgg
     8641 acttgcagcc ggcatcgcac tctcggcgcg ggcgatcggc gtcacgctct cgcccgtcga
     8701 tgcgatcgtc gtgctgaccg gcattgtcat cgctggggcc ataccgttgt cggtcggagg
     8761 ctgggggctc cgagaagggg caaccgtgct cctcctgaag gctgccggcg tcgaggcggc
     8821 ggcggcagta agtctcgcga tactgtttgg cctcgcgctc accgtcctcg gcatcgccgg
     8881 cggcgccgta tggctgacgt cgggctaccg tcgcgtcgat cgcgcgacgg ggcttggcga
     8941 gctgaaacgt gctgcgacag gagcgccgcc atgatcaccg ttccgggcga aggcacgcgc
     9001 gccgggccag agctcacccc ccgtaagcag gccttccgcg acaatgccga ccgcatggcg
     9061 ccggagcgcc gcaagtgggt gcagcgcaat cgcggctact acggcgacga ccgtcgctac
     9121 atgcgcttcc tgatcccacg cggcgcgcgc gtgctcgatc tcggctgcgg caacggcgac
     9181 ctcctcgcgg cacttcaccc ctcccgcggc ctcggtatcg acttttcgcc acgcatgatc
     9241 gaagaggcgc gccgctcgca tccgaacctc gagttcatgc tggcggatgc cgagaatgcg
     9301 agcgagatgg cggcaatcga agggccgttc gacttcatca tcctgtccga cacgatcggc
     9361 acctttgagg atatcgagga ggcgctccgg ctgctgcacc gactctgcac gccctcgacg
     9421 cgtatcgtca tcgcctacta ctcgccctcc tgggacccgc tccttcgcct cggcacgatg
     9481 ctcggcaagc gcatgccgca accgaagccg aacctgatct ccatggttga cttcctcaac
     9541 atcctcgatc tcgccgagtt cgagccgatc cgcatcgaga ggaagcagat cgtgccgctg
     9601 tggctgttcg ggctcgggcg cctcatcaac cggttcatcc agccgctgcc cggaatccgg
     9661 tctttcgggt tgcgcatgta cgtcgtcgcc cgctcgaagc gggctgcacc gaagcccgcg
     9721 ccctcgacgt cggtcgtcat tccctgccgc aacgagaagg gcaacatcga gcgcgccgtc
     9781 cgtgagctgc cgcgatttgg ccgccaccag gagatcgtgt tcgtcgaggg caactcgagt
     9841 gacggcacct atgaggagtg cctgcgcgtc aaggaggctt accagggcca atgggacatc
     9901 aaggtcttga agcaggacgg ccgcggcaag ggcgacgccg tgcgcaaggg cttcacccat
     9961 gcctcgggcg acatcctcat gatcctcgat gccgatctca cgatgccggc gggcgccctg
    10021 ccgaagttct acgatgcgat cgcgacaggg aagggcgagt tcatcaacgg aacgcgcctc
    10081 atctatccca tggaggacga ggcgatgcgc ttcctcaacc atgcggccaa ccgcacgttt
    10141 gcggcgctct tctcctattt gctgaaccag cgctttacgg acacgctctg cggcaccaag
    10201 gtgctgtggc ggacggacta cgaccagatc gtcgccaacc gcgcctattt cggcgacttc
    10261 gatcccttcg gcgacttcga cctcatcttc ggcgcggcaa aacgcaacct gcggatcgtc
    10321 gaggtgccga tccactacaa ggcgcgcacc tacggcgaga cgcagatctc gcgcttccgt
    10381 gacggttggc tcttgctgcg catggtggtt ttcgccttca agaagctgaa ggcgatctga
    10441 ccgtcgccac ctgactttcg agacggccca tcaaatcttc tatccgatgc ttcccgacgg
    10501 ttggccggaa tcgcaccttt cgtgcgcgcg ggcggcaccg cggggctctt ctccgcgatc
    10561 ctgcccggct ggagccccgg cggcgccaac tcgcaggtgg tgaccccggc ctctcgggat
    10621 tgagcagcgg gcgccgaccc gctgacggtg tcggcgaggc gcttgctgcc tcgcgtactt
    10681 gccaaggcat gtgttgaggg ttcgctccgc ctctcactcg tcagggccgc gcaggcggcc
    10741 gcatgcggct cctggtgtag cgcgcgcgat ccgatgccag accgattatc ctggcacgac
    10801 atcgcccgct gtcatggatg cgagcgcatt cgcgcgctct ccatccatcg gtcgccctcg
    10861 atgttcagct cggagccgcc gtcttcatga cgaaggggtt gccgccggcg ccgacctcga
    10921 ggttcatcca cacgctcttg gtctgcaagt actcgtcgat tgccgtcgcg ccgttcgagg
    10981 cccgagctct tgtagccgcc gaacggcatc gtgaaggaga cggcgcggta ggtgttcacc
    11041 cacacagtgc ccgcgcggat gcgccgcgac atgcggattg cgcggttgat gttctgcgtc
    11101 cacacgcccg agccgaggcc gtagatcacg tcattggcga tctggcccgc ctcctcctcg
    11161 tccttgaact tgatgacgga gagcacgggg ccgaacacct cctcctgcgc gatccgcatc
    11221 ttgttgtgga cgccggtgaa gatcgtaggc ttgacgaagt acttgccgcc gccgtcagcc
    11281 gcagtcgccg ggcccccgcc gagcacacac gtggcgcctt cgcccttggc gatgtcgatg
    11341 tagccgagca ccttctcgta ctgcggcggc gtcgtcaccg ggccgacctg cgtctcgaag
    11401 agttgcggat tgccgagctt ggccgtgccc gcgattttga cgagcttctc catgaacttg
    11461 tcgtggatgc tctcctgcac gaggaggcgc gagcccgcga tgcaggtctg ccccgaggcc
    11521 gcgaagatcc ccgagatggc gccattcacg gcgtcgtcga gcacgcaatc gtcgaacacg
    11581 atgttcggcg acttgccgcc gagctcgagc gacaccttct tgaaggcggc agacgcaagc
    11641 gaggcaacgc gcgaacctgt cgccgagccg ccggtgaagg cgaccttggc gaccttagga
    11701 tgcgtgacga gcgcttcgcc cgcaaccgcg ccatagcccg tgaccacgtt gacgacgccg
    11761 ggcggtaggc ccagcggctc gatgacgagc ttcatgaatt cgagaagcga ggcgctcgtg
    11821 tactcggagg gcttgatgac gaccgtgcag ccagtcgcga gcgccggggc aagcttccag
    11881 gcgacgagca ggaggggcga attccacggc accacgaaag cgcacacgcc cacaggctcg
    11941 cgcaacgtga aggtgaagtg gttcgccttg tcgaccggca ccaccgagcc cgtgattttg
    12001 tcggcgagcc cgccgaagta atagtaccac tcgggcatgt acttcgtctg tgcctgcatc
    12061 tccgagatga gcttgccgtt gtcgcgcacc tcgatcttgg cgaggtgctc cgcatgcttc
    12121 tcgatgtttt cggcaagttt cctcaggatt ttgccgcgcg ccgtctgcgt catgctggcc
    12181 caagggccgc tttcgaatgc gcggtgtgcg gcgaggcagg cgcggtcgac atcgcgggcg
    12241 tcgcattctg ggatctctgc ccagggctct cccgaataag ggtccgacgt gagaaagcgc
    12301 ttgcccgagg ccgcgtccgt ccacttgcca tcgatgtagg tctggtaggt ctgcagcttg
    12361 gtggggcgat cctgcatggc atgtctccgg tgctggggat ttgagccgat ccaacgactg
    12421 tcgcggccgg acgtcaagcg ccgcggctcc tgatcagctg cgtggcttgg cgcgtgtggt
    12481 tggcggcgct gtggccgggt cttcgggcca ggggtgcttc ggatagcggc cccgcatctc
    12541 ggcgcgcacc gccgcccacg aacccttcca gaagccgggg agatcgcggg tgatctggat
    12601 cgggcgcccc gccggcgaga ggagatgcaa cgtgaggggc aagcggccgc gcgcgatcgc
    12661 agggtggacg gtcaacccga aaagctgctg cacgcggatc gccaggatgg gcgctcccgg
    12721 cccctcgtac tcgatcgggt gacgctggcc ggtcggcgcc tcgtagtggg tcggcgcatc
    12781 gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnatcgg gcaggccgag gcgcggctcg gcggcggcga
    12961 caaagccgac gcggtcacgg agttggcgct gggcgcggct ccaggggagc ttgtcgatgc
    13021 cgagagtggc ggcgcctttt gccagcgcca gggcggaggc ggcatcgatc gcgacggcgg
    13081 ccggagtgct ctcgagcgtg atggcatcga gcgaccgggt gcggcgcgcg cggaccgctt
    13141 tcgcctcggc gtcgaagaat acttcgtcgc gctcgct
//
LOCUS       DAORNH010000106        13158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3575_length_13158_cov_2.622606, whole genome shotgun sequence.
ACCESSION   DAORNH010000106 DAORNH010000000
VERSION     DAORNH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_13158_cov_2.622606"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>231)
                     /locus_tag="PK264_12095"
     CDS             complement(<1..>231)
                     /locus_tag="PK264_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRD76662.1"
                     /translation="PVTKRDRAIVLQLALGELLIRGGERIGLLGVGPPSASRRATTRM
                     AERIAQNATRPELTAGLPPKARLSRFSGTVLF"
     assembly_gap    232..422
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(618..1625)
                     /locus_tag="PK264_12100"
     CDS             complement(618..1625)
                     /locus_tag="PK264_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRD76663.1"
                     /translation="MSTVTQTDDNIVERVEAAGERIHRVHKAVSAVIFGQDQVIERTL
                     VTLLSGGHALLVGVPGLAKTLLVETLGKALGLDAKRIQFTPDLMPSDIIGAEVLEENQ
                     TGSGGRRSFRFIKGPIFAQLLMADEINRASPKTQSALLQAMQEHHVTVAGQRHDTPQP
                     FHVLATQNPLEQEGTYPLPEAQLDRFLLQIDVGYPDQAAERRMLFSTTGVEDRKVETV
                     FTGPELMAVQRLVRQIPVPDKVVEAILALVRSARPGSGAGEETDRLIAWGPGPRASQA
                     LMLAVRAKALIDGRLAPSIDDVISLADPILKHRMALTFAARAEGETVGGVIARLAGRV
                     S"
     gene            1774..2337
                     /locus_tag="PK264_12105"
     CDS             1774..2337
                     /locus_tag="PK264_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HRD76664.1"
                     /translation="MAQSDEKAGGLAGLEALLKAQGTLPPVERWNPPYCGDIGMRIRS
                     DGVWLYQSSPIGRMPLVKLFARVLRRDADGRHYLVTPVEKVDVAVDDAPFLAVEMEIR
                     GDGEAREFLFRTNVDDVVALGREHPLRFVEEAGSGGLKPYLHVRGRLEALVTRALYYD
                     LVELAEPRADGTLVLTSGGVEFPLVAG"
     gene            complement(2351..3142)
                     /locus_tag="PK264_12110"
     CDS             complement(2351..3142)
                     /locus_tag="PK264_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD76665.1"
                     /translation="MPVYETLEVAIGADRVGLITLNRPDALNAMNTQMMRELRDLFMT
                     FYVDESQAACLVLTGSGTRGFCTGADLKERKGMSDSTWRQQHAIVEQMVRAIMECPIP
                     VIAAVNGHAYAGGMEIALACDFVYAAEHARFALTEVTLGIMPGAAGTQNLPRAVGVRR
                     AKEIILTGTPFTAADALGYGMVNAVHPGDRLLEAALATARKIASNAPISVRQAKKSID
                     KGVEMDRAMGYAFEIEAYNRTVPTEDRQEGINAFNEKRKPQYKGR"
     gene            3702..5033
                     /locus_tag="PK264_12115"
     CDS             3702..5033
                     /locus_tag="PK264_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD76666.1"
                     /translation="MNGRSPTTVTRANPIDRRTLLRQGAATAATAGLMSSLKAAFPAG
                     AFAQAAGPEVKGTRLGYIALTDAAPLIIAKEKGFYARHGLPDMDIAKQASWGATRDNM
                     ALGFKANGIDGGHILRPKVHLYATGAVMQNKQPLAMYTLLNLNEDCQGLSVANEYKAL
                     PVGTDASPLKEAFARKKAAGKEVKVAMTFPGGTHDLWIRYWLAAGGIDPDKDVQTIVV
                     PPPQMVANMKVGTMDAFCVGEPWNEQLVNQDIGFTAATTGEIWFKHPEKVLGMRADFV
                     DANPKATLAILAATMEAQMWCEKMENKEEMAAIIGKRQWFNVPVADIIGRIKGDINYG
                     RGKVAKGTNLLMKFWGDGGAVSYPWKSLDTWFVAENIRWGKFAPDTDIKALVNKTNRS
                     DLWLAAAKSLGVAGAPSGDSRGVEKFFDGKTFDPADPAAYLKSLAIKRATV"
     gene            5193..6017
                     /gene="ntrB"
                     /locus_tag="PK264_12120"
     CDS             5193..6017
                     /gene="ntrB"
                     /locus_tag="PK264_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007634577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HRD76667.1"
                     /translation="MEPRKGARKRQPMKHLAAIIVAVLPPLIVVALALLVWEVAASGP
                     KSSLPSPSRIWVEARDLIVNPFFVNGTQDIGLGWRVLTSLQRVAIGFGLAAVVGILLG
                     ALVGQSVWAMRGLDPVFQVLRTVPPLAWLPISLAAFRDANPSAIFVIFITSIWPIIIN
                     TAVGIRNIPQDYRNVADVLRLNQAEFFWRIMIPSAAPYMFTGLRIGIGLSWLAIVAAE
                     MLTGGVGIGFFIWDAWNSSRLSDIVVALAYIGIVGFVLDRIVALVGRIATRGTATA"
     gene            6074..>6608
                     /locus_tag="PK264_12125"
     CDS             6074..>6608
                     /locus_tag="PK264_12125"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76668.1"
                     /translation="MGAGRPLLKIDHVDKTFSRGGTDTEVLRDVSLAIERGEFVSIIG
                     HSGCGKSTLLNLIAGLTRVSRGAVLLEDQEVNAPGPDRAVVFQNHSLLPWLTVYENVR
                     MAVDKVFSGHKSRTERHEWTLHNLGLVQMGHAKDKRPGEISGGMKQRVGIARALAMEP
                     KVLLMDEPFGALDALTRA"
     assembly_gap    6609..6676
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6677..6885
                     /locus_tag="PK264_12130"
     CDS             <6677..6885
                     /locus_tag="PK264_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76669.1"
                     /translation="DVDEAVLLSDRIVMMTNGPAATIGEVLDVDLPRPRRRLELVSDP
                     TYIAAREAVLRFLYERHRYVEAAA"
     gene            6894..9376
                     /gene="nirB"
                     /locus_tag="PK264_12135"
                     /pseudo
     CDS             6894..9376
                     /gene="nirB"
                     /locus_tag="PK264_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
     gene            9451..9780
                     /gene="nirD"
                     /locus_tag="PK264_12140"
     CDS             9451..9780
                     /gene="nirD"
                     /locus_tag="PK264_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HRD76670.1"
                     /translation="MWVDIGALDSVPVRGARVVKTAQGCVAVFRTGESEVYAIDDRCP
                     HKAGPLSQGIVHDKAVTCPLHNWVISLESGEATGADTGRVRTYALKVEAGRILLDTSA
                     LKPCKVT"
     gene            9806..10285
                     /locus_tag="PK264_12145"
     CDS             9806..10285
                     /locus_tag="PK264_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD76671.1"
                     /translation="MGTLRIENLEFRLDDLTGAPIRALIARHLRGMHENSPPESVHAF
                     DIDKLRAPDVTFWSAWSGGEIAGCGALKMLSATEGEIKSMRVADSYLGQGVGRAILEH
                     IVSEARLRSLASLWLETGSAPAFAPAIGLYESAGFTRCPPFADYAPDPFSVFMTRAL"
     gene            10298..12982
                     /locus_tag="PK264_12150"
     CDS             10298..12982
                     /locus_tag="PK264_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HRD76672.1"
                     /translation="MTAVTRTTCPYCGVGCGILATRTAAGGVEVRGDPDHPANFGRLC
                     SKGSALAETLSLDDRLLYPLVDGVRASWDTALDRVATRFAEAIAVHGPEAVAFYVSGQ
                     ILTEDYYVANKLMKGFIGAANIDTNSRLCMASSVAGHKRAFGADAVPGCYEDLEAADL
                     VVLVGSNLAWCHPVLYQRIVAAREKRGGLPKVVVIDPRRTATSEIADMHLALKPDSDV
                     ALFNGLLLALAGGNATDRDWISRCTSGFRAAIDAARAMSVSDISRASGLAKGELLAFY
                     DMFVRTERVVTVYSQGVNQSASGTDKVNAIINCHLATGRIGKPGMGPFSVTGQPNAMG
                     GREVGGLANMLAAHMDIENAAHRAIVQDFWRAPTIATKPGLKAVDMFRAVGDGRIKAI
                     WIMATNPVDSMPEADAVRAALAACPFVVVSDVAHHTDTTALGHVLLPSLAWGEKDGTV
                     TNTERCISRQRAFLKPPGDARADWWQLAEVGKRMGFGDAFAWETAAAIFDDYARLTAL
                     ANDGTRDLDLEGLAGLTAADYDALAPVQWPVARTRCQTHGSDTAPAKRLFANGQFYTP
                     DGRARFVPTPYRAPAASEELSFVLNSGRIRDQWHTMTRTSKTARLMSHIGEPFVEVHP
                     VDAARLGMAPAEIARIASADRAMLARVVVTSRQQPGSVFVPMHWTDQLTNGGRVDALV
                     AAVVDPVSGQPALKQARVTVAAFPARWYAVAVTRERPANIAVDYWAIAPVAGGWRIEM
                     ADGGALGGSAVVLADLLPSEASNPADVVSYVDRASGQSRSAAFAGDRLQAILYVAAEP
                     VAVSRVWLAEHLARDIASPADRLRLLAGRPGAGERDAGPIVCACFAVGANDIAAAVAR
                     GSTSIAAVGAATSAGTNCGSCRAEIGRLIHAARVAKAG"
     gene            12954..>13158
                     /locus_tag="PK264_12155"
     CDS             12954..>13158
                     /locus_tag="PK264_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76673.1"
                     /translation="MQLVSRKPVDVAPPRMGALAKLPVFLDLDGKRAVVAGGTAGAAW
                     KAELLAAAGADVHVYAGGPGEEMA"
BASE COUNT         2120 a         4190 c         4465 g         2124 t
ORIGIN      
        1 agaatagcac ggtgccggaa aagcgcgaga ggcgcgcttt cggcggcagg ccggcggtga
       61 gctcgggccg cgtcgcgttt tgcgcgatgc gctcggccat gcgcgttgtc gcgcggcggc
      121 tggcggaggg cgggccgacg ccgagcaagc cgatgcgctc gccgccgcgg atgaggagct
      181 cgccgagcgc gagctggagc acgatcgcac gatcgcgctt ggtgaccggg gnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnccggcgcg gcggcggccg tggatgccgt gcgtcaccgt catggagacg cgcatcgcct
      481 ccatgacgag atcggggagg cgatcggcga cgccatgtgc ctcgccctcg aggcgcagca
      541 cgctggtgtc cgcagcgccg gccgcagcac tcgtcgtccc tccgcgtgtc gccgccatca
      601 tcgcctccgc gcgctcttca gctgacgcgg ccggcgaggc gcgcgatgac gccgccgacc
      661 gtctcgccct ccgcacgggc ggcgaacgtc agggccatgc ggtgtttcag gatcgggtcg
      721 gcgagagaga tcacatcgtc gatggagggg gcgaggcggc cgtcgatcag cgccttggcg
      781 cggacggcga gcatgagagc ctggctcgca cgggggcccg gaccccaggc gatcagacgg
      841 tcggtttcct cgcctgcgcc agagccggga cgggcagagc ggacgagggc aaggatcgcc
      901 tcgacgacct tgtcggggac ggggatctgg cgcacgagcc gctgcacggc catcagctcg
      961 ggacccgtga agacggtctc gaccttgcgg tcttcgacgc ccgtcgtcga gaacagcatg
     1021 cgtcgctcgg cggcctgatc gggatagccg acgtcgatct ggagaaggaa gcggtcgagc
     1081 tgggcttcgg gcagcgggta ggtgccttcc tgctcgagcg ggttctgcgt ggcgagcacg
     1141 tggaagggct gcggggtgtc gtggcgctgg ccggcgacgg tgacgtgatg ctcctgcatg
     1201 gcctgcagca gcgccgactg cgtcttgggg ctcgcgcggt tgatctcgtc ggccataagg
     1261 agctgcgcga agatcggtcc cttgatgaag cggaagctgc ggcgcccgcc gctgcccgtc
     1321 tggttctcct cgagcacctc cgcgccgatg atgtcggagg gcatcaagtc aggcgtgaac
     1381 tggatgcgtt tggcatcgag gccgagcgcc ttgccgagcg tttcgacaag cagcgtcttg
     1441 gcaaggccgg ggacaccgac gagcagcgcg tgaccgcccg aaagcagcgt gaccagcgtg
     1501 cgctcgatca cttgatcctg gccgaagatg acggctgaca ccgccttgtg gacgcgatgg
     1561 atgcgctcgc cggcggcttc gacgcgctcg acgatgttgt cgtcggtctg tgtgacggtg
     1621 ctcatgggat ttgggcgacc tgcggttggt tgaccgggga gcttgctgcc ccgaacatgg
     1681 tgatactatg gactatcggc gaccttagca gcagggtgca agggcgtcgt cttaacaacc
     1741 cgtaatctgg cgaaatcgtg aagagtggcc ttgatggcgc agtcggatga aaaggcgggc
     1801 gggctcgccg ggctcgaggc gctcttgaag gcgcaaggca ctctgccgcc cgtcgagcgc
     1861 tggaacccgc cctattgcgg cgacatcggc atgcgcatcc gctccgacgg cgtgtggctc
     1921 taccagagct cgccgatcgg gcggatgccg ctggtcaagc tgttcgcgcg cgtgctcagg
     1981 cgtgacgcgg acgggcggca ctatctcgtg acgcccgtcg agaaggtaga cgttgctgtg
     2041 gatgacgcac cgttcctcgc ggtcgagatg gaaatacgcg gggatggcga agcgcgcgag
     2101 ttccttttcc gcaccaacgt ggacgacgtg gtggcgctcg gccgcgagca tcccctgcgc
     2161 ttcgtcgaag aggccgggag cggcgggctg aagccctatc tccacgtccg tggccgcctc
     2221 gaggccctcg tcacgcgcgc gctctattac gacctcgtcg agctcgccga gccgcgcgcg
     2281 gacgggacac tcgtgctcac cagtggcggc gtcgagttcc cgctggtcgc gggctgagac
     2341 cccggccggg tcagcggcct ttgtattgcg gcttcctctt ctcattgaag gcgttgatgc
     2401 cctcctggcg atcctccgtc ggcaccgtgc gattgtaggc ctcgatctcg aaggcgtagc
     2461 ccatggcgcg atccatctcg acgcccttgt cgatggactt cttggcctgg cgcacggaga
     2521 tcggcgcatt cgaggcgatc ttgcgggccg tcgccagcgc ggcctcgagg agcctgtcgc
     2581 cggggtggac tgcattcacc atgccgtagc cgagcgcgtc ggcggccgtg aacggcgtgc
     2641 ccgtgaggat gatctccttg gcgcggcgca cgccgacggc gcgcggcagg ttctgcgtgc
     2701 ctgcggcgcc cggcatgatg ccgagcgtca cctcggtcag cgcgaagcgg gcatgctcgg
     2761 ccgcgtagac gaaatcgcag gcaagcgcga tctccatgcc gcccgcgtag gcgtgaccgt
     2821 tgacggcggc gatgacgggg atcgggcact ccatgatggc gcgcaccatc tgctcgacga
     2881 tggcgtgctg ctggcgccag gtcgaatcgc tcatgccctt gcgctccttg aggtcggcgc
     2941 cggtgcagaa gccgcgggtg cctgatcccg tgaggacgag gcaggccgcc tggctctcgt
     3001 cgacgtaaaa ggtcatgaag agatcgcgca gctcccgcat catctgcgtg ttcatggcat
     3061 tgagcgcgtc gggccgattg agggtgatga ggccgacgcg gtcggcgccg atcgcgacct
     3121 cgagcgtctc gtaaacgggc atggggcatc ctttgcgggt catgacaagg cgccactctg
     3181 cccgcagtgc agggcaggat caatccgatg cggcaggtga gtagggagtg ggcgaactat
     3241 gcgaggagga gcctgctagc tcgacggtga gggggaggtt cgggcacgtg gaccggccaa
     3301 cgctcgtcag gcgcgtggtc ggtcgtgcac gaatttcagg ctgacctatt gcgctgcaca
     3361 attcattggc gaagcgctca taaagccggc gacctccacg ccgaaatgta cgcgtttcgg
     3421 tgcctggtag atcgtcatag aggtttgaaa tataaagtaa ttcagtcgaa attttaaaac
     3481 tggcacggat cctgccttaa ccattgccga cgcgccggct cccggcgtct cggtgggatg
     3541 aatgctgtgc cttgcggcag cgctgctgcc agcggtcgct gcgttgcaca caagcgtgcc
     3601 ggcctcgatc cggcagcgcg gtggtcgcgg agcgccggca ctcgctccct caccgggatg
     3661 ccccgtcggc ccgagctgac aacgcgggac agaggtggga catgaacgga cgatccccga
     3721 cgacggtgac gcgggccaat cctattgatc ggcgcacgtt gcttcggcag ggtgccgcca
     3781 cggcggcgac tgcggggctg atgtcgtcgc ttaaggcggc gtttcctgct ggcgcgtttg
     3841 cacaagcggc tgggcctgag gtgaagggga cgcggctcgg ctacattgcg ctcaccgatg
     3901 ccgcgccgct gatcatcgcc aaggagaagg gcttctacgc ccggcacggc ctccccgaca
     3961 tggacatcgc caagcaggcc tcgtgggggg cgacgcggga caacatggcg ctcggcttca
     4021 aggccaacgg catcgacggc ggccacatcc tgcgacccaa ggtgcacctc tacgcgaccg
     4081 gtgcagtgat gcagaacaag caaccgctcg ccatgtacac gcttctcaac ctcaacgagg
     4141 actgccaggg cctctctgtc gccaacgagt acaaggcact gcccgtcggc acggatgcct
     4201 cgcccttgaa ggaggccttc gccaggaaga aggccgcggg caaggaggta aaggtcgcca
     4261 tgaccttccc tggcggcacg cacgacctgt ggatccgcta ctggctcgcg gccggcggca
     4321 tcgatcccga caaggacgtg cagacgatcg tggtgccgcc gcctcagatg gtcgccaaca
     4381 tgaaggtcgg caccatggat gccttctgtg tcggcgagcc ctggaacgag caactcgtca
     4441 accaggacat cggcttcacg gcggcgacca cgggcgagat ctggttcaag caccccgaaa
     4501 aggtgctcgg catgcgggcc gatttcgtcg acgcgaaccc gaaggcgacg ctcgctattc
     4561 tcgcggccac gatggaagcg cagatgtggt gcgagaagat ggagaacaag gaggagatgg
     4621 ccgcgatcat cggcaagcgg cagtggttca acgtgccggt cgccgacatc atcggacgca
     4681 tcaagggcga catcaactac ggccgcggca aggtcgccaa ggggaccaac ctgctcatga
     4741 aattctgggg cgacggcggc gccgtctcct atccctggaa gagcctcgat acctggttcg
     4801 tcgcagagaa catccgctgg ggcaagttcg cgccggacac cgatatcaag gcgctcgtca
     4861 acaagaccaa tcgctcggat ttgtggctcg cggcggcgaa gtcgcttgga gttgcgggcg
     4921 cgccctcggg cgacagccgc ggagtcgaga agttcttcga cggcaagacg ttcgatccag
     4981 ccgacccggc ggcctacctc aagtcgctgg ccatcaagcg cgcgacggtg tgagcgctgg
     5041 agcggtgccc tcgatacggg cgccttccac cggcctctca ttgcatgatc aatcggagct
     5101 tctgaatgcc gtcacacgcc gtcaagcttg caacgacagg ttcagccgcc gccatacccg
     5161 tgagcgccgc accgcacgcc gagatcatcg ccatggagcc gcgcaagggc gctcgcaagc
     5221 gccagcccat gaagcacctc gctgcgatca tcgtagcggt gctgccgccg ctgatcgtcg
     5281 tcgcgctggc gctgctcgtc tgggaggtcg cggcatcggg tcctaagtcg agtctgccgt
     5341 cgccgagcag gatttgggtc gaagcgcgtg acctgatcgt caatccgttc ttcgtcaatg
     5401 gcacgcagga catcggcctc ggctggcgcg tgctgacctc gttgcagcgc gtcgcgatcg
     5461 gcttcggtct agcggcggtc gtcggcatcc tgctcggcgc gctcgtcggg cagagcgtgt
     5521 gggcgatgcg cgggctcgat cctgtgttcc aggtgctgcg aaccgtgccg ccgctcgcct
     5581 ggctgccgat ctcgctcgcg gcgttccgcg atgccaaccc ttcagcgata ttcgtgatct
     5641 tcatcacctc gatctggccg atcatcatca acacggcggt cggcatccgc aacattccgc
     5701 aggactaccg caacgtggcc gacgtcttgc ggctcaatca ggcggagttc ttctggcgga
     5761 tcatgatccc gtcggcggca ccctacatgt tcacgggcct caggatcggc atcggccttt
     5821 cctggctcgc gatcgtcgcg gccgagatgc tgaccggcgg cgtcggcatc ggcttcttca
     5881 tctgggatgc ctggaattcc tcccgtctct ccgacatcgt cgtcgcactc gcctacatcg
     5941 gaatcgtcgg cttcgtgctc gaccgcatcg tcgcgctcgt cgggcgtatc gcgacgcgtg
     6001 gcacggccac ggcttgaagg gacacatcat gatcgccaag ctcgagccga gtttgcatac
     6061 gacgtcccgc ccgatgggcg cgggccgacc gctcctcaag atcgaccatg tcgacaagac
     6121 cttctcgcgc ggcggcacgg acaccgaggt gctgcgtgac gtctcgctcg cgatcgagcg
     6181 gggcgagttc gtctcgatta ttggccactc gggatgtggg aagtcgacgc tgctcaatct
     6241 gatcgcgggg ctgactcggg tctcgcgcgg cgccgtgctg ctcgaggacc aggaggtgaa
     6301 tgcgccggga cccgatcgcg ccgtcgtctt ccagaaccac tcgctgctgc cgtggctcac
     6361 ggtctacgag aacgtgcgca tggccgtcga caaggtgttc tccggccaca agtcgcgcac
     6421 cgagcgccac gagtggacgc tgcacaacct cggcctcgtg cagatggggc acgccaagga
     6481 caagcgtccg ggcgaaatct ccggcggcat gaagcagcgc gtcggcatcg cccgcgcgct
     6541 cgcgatggaa ccgaaggtgc tgctgatgga cgagcccttc ggcgcgctcg atgcgctgac
     6601 gcgggcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnacga cgtcgacgag gccgtgctgc tctccgaccg catcgtgatg
     6721 atgaccaacg gtccggcggc gacgatcggc gaggtcctcg atgtcgatct gcctcggccg
     6781 cgccggcgcc tcgagctcgt ctcggacccg acctacatcg ccgcgcgcga ggcggtgctc
     6841 cgcttcctct acgagcggca ccgatacgtg gaggcggcgg catgagcacg gagatgctgg
     6901 aaaggtcgac gccgcgcgtc gcgccgcctg agcggcagcg gctcgtcgtg atcggcaacg
     6961 gcatggcgcc ggggcgcgcg ctagagaagc tcttcgagct cgcgccggac gcctatgacg
     7021 tcacgatctt caacgccgag ccgagggtca actacgatcg catcatgctc tcgcctgtgc
     7081 tctcgggcga gaagagcttc gagcagatcg tcatccacgg cgacggctgg tacgtgaagc
     7141 acggcgtgac gctgcacaaa ggcgtcagcg tgaccggaat cgatcgcgcg acgcggacgg
     7201 tcaccacggc gcgcggcatg acggtgccgt acgaccggct catcatcgcg accggctgcc
     7261 gatcgtcatt cccgtaccgg gccacaagct cgcgggcgtg ctctcctacc gcgatctcga
     7321 cgacgtgcag gcgatgctga tcgccgccaa gtcgcgcggc aaggcgatcg tcatcggcgg
     7381 cgggctcctc ggcctcgagg cggccgtcgg cctcaaggag cagggtatgg atgtcaccgt
     7441 cctgcacctc gcgccgacgc tgatggagcg ccagctcgac acgccggcag gtcatctcct
     7501 ggagaaggcg atcgaggcgc gcggcataag cgtgatcacg cgcgcgaaca ccaaggctat
     7561 cctcggcgag aagaaggtcg aggccgtgct gctcgaggac ggcacgcggc ttgccgccga
     7621 tctcgtcgtc atggccgtcg gcatcaagcc caacgcggcg ctcgccaagg aggcggggct
     7681 cacggtcaac cgcggcatcg tcgtcgatgc ggcgatgcga acgtcggacg cggacatctt
     7741 cgccgtcggc gaatgtgccg agagccacgg acaggtgttc gggctcgtgg cacccctcta
     7801 cgagatggcg agcgtgatcg ccggtcagct cgcgggcgat gccaacgccg cgttcagtcc
     7861 gagcgcggtc gcgaccaagc tgaaggtcac gggcatcaac ctcttctcgg cgggcgactt
     7921 cgcagaggcg aaggaccgcg aggagatcgt gctccgcgat gcggcgcgcg gtgtctacaa
     7981 gcggctcgtc atcaaggaca acaagctcat cggcgccgtg ctctacggag agacgggcga
     8041 cggcgcctgg ttcttcgacc tgataaaaaa aggcacgccg atcaaggagt tgcgtgagac
     8101 gttgatcttc ggcccgagct atgccggagg cagccctctg gaccctacgg cggccgttgc
     8161 agcactgccg gatgaggcag agatctgcgg ctgcaacggc gtctgcaagg gcaggataac
     8221 gggcgcgatc acatcgctga agctctcgtc gctcgacgac gtgcgcgccc acacgaaggc
     8281 ctcggcgagc tgcggctcgt gcacgggcct cgtcgagcag ctgatcaagc tgacactcgg
     8341 cgacggctac aatccgtcgg ccgtgcagcc gatgtgcaag tgcacgaccc tcggccacga
     8401 cgacgtgcgc cggctgatcg tcgcgaaggg cctgaagacg atccctgacc tgatgcaggc
     8461 gctcgagtgg acgacgtcgt gcggctgcgc caagtgccgg cccgcgctca actactacct
     8521 gctcgcgacc tggcccggcg agtatcgcga cgacaatcaa tcgcgcttca tcaacgagcg
     8581 cgtgcacgcc aatatccaga aggacggcac gtactccgtc gtcccgcgca tgtggggcgg
     8641 gatgacgagt gccactgagc tccgcgccat cgccgacgtg gtcgagaaat tcaagatccc
     8701 ggcggtcaag tgcaccggcg gccagcgcat cgacatgctc ggcgtgaaga aggaggacct
     8761 gcccgcggtc tgggccgatc tcaacaaggc gggcatggtg tcgggagcgg cgtacgccaa
     8821 ggggctgcgc acagtgaaga cctgcgtcgg cacggactgg tgccgcttcg gcacgcagga
     8881 ttcgactggc ctcggcgtga agctcgagaa gttcctctgg ggctcgtgga ctccggccaa
     8941 gctgaaactc gccgtatccg gctgcccgag gaactgcgcc gaggcaacct gcaaggacat
     9001 cggcgtcatc tgcatcgaga gcggctacga catccacttc gcaggcgcgg ccggcctcga
     9061 catcaaaggc acggagctgc tcggccatgt ggcgacggag gccgagacaa tcgaggtgat
     9121 cgcggcgctg acgcagctct accgcgagca gggccgctat ctcgagcgca tctacaagtg
     9181 ggcaaagcgc gtcggcgtcg agacgatccg ccgccaggtg atggacgaca cggagaagcg
     9241 cgcggcgctg cattcgcgct tcgttcattc gcagaagttc gcacagatcg atccttgggg
     9301 ccagcgcgtc gcggggaagg actcgcacga gttctccccg ctcgcggatt tgacgttcaa
     9361 ggaggctgcg gaatgacatc ggcagcagtc agggtcgcgt cggcggcttc cgggcgggtt
     9421 cccgcaggag agccggacgg cgccacccga atgtgggtgg acatcggcgc gctcgactcg
     9481 gtcccggttc gcggcgcgcg cgtcgtgaag acggcgcaag gctgcgtcgc cgtgttccga
     9541 acgggcgaga gcgaagtcta cgcgatcgac gacagatgcc cgcacaaggc tgggccgctc
     9601 tcgcaaggta tcgtgcacga caaggccgtg acctgcccgc tacacaactg ggtgatcagc
     9661 ctcgagagcg gcgaggcgac aggcgcggac acggggcgtg tgcgcacata tgcgctgaaa
     9721 gtggaggcag gccgcatcct gctggatacg tcagcgctca agccgtgcaa agtcacctga
     9781 gagtcgacac atcaggggca gcagcatggg cacgttgaga attgagaacc tggagtttcg
     9841 ccttgacgac ctcacgggcg cgccgatccg cgccctgatc gcgcgtcacc tgcgcggtat
     9901 gcatgaaaat tcgccgccgg aaagcgtcca cgccttcgac atcgacaagc tgcgcgcgcc
     9961 ggacgtgacc ttctggtcgg cctggagcgg cggcgagatc gccggttgcg gcgcactcaa
    10021 gatgctgagc gcgaccgagg gcgagatcaa gtccatgcgc gtcgccgatt cctatcttgg
    10081 ccagggcgtg gggcgcgcga tcctcgagca catcgtgtcc gaggcgcggc tgcgctctct
    10141 cgcgtcgctc tggctcgaaa cgggttcggc gccagccttc gcacctgcca tcgggctcta
    10201 cgagagcgcc ggctttaccc gctgcccgcc ctttgcagac tacgcccccg accccttcag
    10261 cgtcttcatg acgcgcgcgc tctgaccgag gacccgcatg acagccgtga cgcgcaccac
    10321 ctgcccctac tgcggtgtcg gctgcggcat cctcgcgacc cgcacggcag cgggaggtgt
    10381 cgaggtgagg ggcgatccgg atcacccggc gaatttcggg cggctctgtt cgaaggggtc
    10441 ggcactcgcc gagacgctct cgctcgacga ccggctgctg tatccgctcg tcgacggcgt
    10501 acgcgcgagc tgggatacgg cactcgatcg cgtcgcgacg cgctttgcag aggcgattgc
    10561 ggtgcacggc cccgaagcgg ttgccttcta cgtctcgggg cagatcctga ccgaggatta
    10621 ctacgtcgcc aacaagctga tgaagggctt catcggcgcg gccaacatcg acaccaactc
    10681 gcggctctgc atggcgtcgt cggtcgcggg gcacaagcgt gcgttcggcg ctgatgccgt
    10741 gcccggctgc tacgaggatc tagaggcggc cgacctcgtc gtgctggtcg gctcgaatct
    10801 cgcctggtgc catccggtgc tctaccagcg catcgtcgcg gcgcgggaaa aacgtggcgg
    10861 gctgccgaag gtcgtcgtga tcgatccgcg gcggacggcg acatctgaga tcgccgacat
    10921 gcatctggcg ctgaagccgg acagcgacgt ggcgctcttc aacggattgc tcttggcgct
    10981 cgccggcggc aacgcgacgg accgcgactg gatctcgcgc tgcaccagcg gcttccgcgc
    11041 cgcgatcgat gcggctcggg ccatgtcggt gagcgacatc tcacgggcat ccggccttgc
    11101 caagggcgag ctgctggcct tctacgacat gttcgttcgg acagagcgcg tcgtcacggt
    11161 ctacagccag ggcgtcaacc agtcggcgtc gggtaccgac aaggtcaacg cgatcatcaa
    11221 ctgccacctc gcgaccgggc gcatcggcaa gcccggcatg ggaccgttct cggtcacggg
    11281 tcagccgaac gccatgggcg gccgcgaggt cggcgggctc gcaaacatgc tcgccgcgca
    11341 catggacatc gaaaacgcgg cgcatcgcgc gattgtccag gacttctggc gtgcgccgac
    11401 gatcgcgacg aagcccggcc tcaaagccgt cgacatgttc cgcgcggtcg gcgatgggcg
    11461 catcaaggcg atctggatca tggcgaccaa tccggttgac agcatgccgg aggcagacgc
    11521 ggtgcgggcg gcgctcgcgg catgcccgtt cgtcgtcgtc tcggatgtcg cgcatcacac
    11581 ggatacgacg gcgctcggtc acgtgctgct gccgtcgctc gcctggggcg agaaggacgg
    11641 cacagtcacc aacacggagc ggtgcatctc gcgccagcgc gcgtttctca agccaccggg
    11701 cgacgcccgg gccgactggt ggcagctcgc cgaggtcgga aagcgtatgg gctttggcga
    11761 tgcattcgcg tgggaaacgg cggcggcgat attcgacgac tacgcgcggc tcacggcact
    11821 cgcgaatgac ggcacgcgcg atctcgatct cgaaggcctc gcggggttga ctgcggcgga
    11881 ttacgacgcg ctcgcccccg tgcagtggcc ggtggcgaga acgcggtgtc aaacccatgg
    11941 gtctgacacc gcgcccgcga aacgcctctt cgcgaatggg cagttctaca cgcccgatgg
    12001 ccgcgcccgc tttgtgccga cgccgtatcg cgcgccggca gcatccgaag aattgtcctt
    12061 cgtcctcaac tccggtcgca tccgcgacca gtggcacaca atgacgcgca cgtcgaagac
    12121 ggcgcgcctg atgagtcaca tcggcgagcc gttcgtcgag gtgcatccag tggatgcggc
    12181 gcgcctcggc atggcgccgg ctgagatcgc gcgcatcgcg agcgccgacc gcgccatgct
    12241 cgcccgcgtc gtggtgacca gccgccagca gccgggctcg gtcttcgtgc ccatgcactg
    12301 gacggatcag ctgacgaatg gcgggcgcgt cgatgcactg gttgcagctg tggtcgatcc
    12361 ggtttcgggg cagccggcac tcaagcaagc gcgcgtcacg gtcgcggcat ttcctgcgcg
    12421 ctggtacgcg gtggccgtca cgcgcgagcg gccggcgaac atcgcggttg actactgggc
    12481 gatcgcgccc gttgctggcg gctggcgcat cgagatggct gacggaggcg cgctcggcgg
    12541 gtccgcagtt gtgctcgctg atctgctccc gtccgaggcg agcaaccctg ccgatgtcgt
    12601 ctcctacgtc gatcgcgcca gcgggcaatc gcggtctgcc gcgttcgccg gcgatcggct
    12661 ccaggccatc ctctatgtcg ctgccgagcc ggtcgcggtg tcgcgcgtct ggcttgccga
    12721 gcatctcgcc cgcgacattg cctcacccgc cgaccgcctt cgcctcctcg ctggacgtcc
    12781 cggcgcgggc gagcgcgatg ccggtccgat cgtgtgcgcg tgcttcgcgg tcggcgccaa
    12841 cgatatcgct gctgctgtcg cgagaggctc gacgagcatc gctgccgtcg gcgcggcgac
    12901 ctctgccgga accaactgcg gctcctgccg cgccgagatc gggaggctca tccatgcagc
    12961 tcgtgtcgcg aaagccggtt gatgtggcgc cgccccgcat gggtgcgctc gccaagctcc
    13021 ccgtattcct cgatctcgac gggaagcgcg cggtcgtcgc gggtgggacg gcgggtgccg
    13081 cctggaaggc ggagctgctc gccgcggccg gcgccgacgt gcacgtctac gcaggcgggc
    13141 cgggcgagga gatggcgg
//
LOCUS       DAORNH010000107        13078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3607_length_13078_cov_2.504262, whole genome shotgun sequence.
ACCESSION   DAORNH010000107 DAORNH010000000
VERSION     DAORNH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13078
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3607_length_13078_cov_2.504262"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..675)
                     /locus_tag="PK264_12160"
     CDS             complement(265..675)
                     /locus_tag="PK264_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76674.1"
                     /translation="MSSNLATRMAEAVPEAWKRWRKARALKAELLALPAGERARVLQE
                     NGLSEFDLERIDVNHEGPQELLPRRLEAIGLDKVTIAAAEPAVARDLERVCSRCGQPG
                     RCAFDLEQEDAAERLARYCPNTGTIDALKLEKSR"
     gene            821..2170
                     /gene="hemN"
                     /locus_tag="PK264_12165"
     CDS             821..2170
                     /gene="hemN"
                     /locus_tag="PK264_12165"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HRD76675.1"
                     /translation="MHTPLTPEAIERLAAPVPRYTSYPTAPHFSGAIDARAYAGWLES
                     LPADASLSLYLHIPYCQTLCWYCGCNTKATQRYQPVSTYLQALRREIGAVARLLPAVH
                     GVAHIHFGGGSPNIVGAADFETLVAQLRREFTVAADAEIAVEIDPRHCNDGYAAALQR
                     AGITRVSIGVQDFDPEVQAAINRIQSFEATARVVRDLRAHGIGSLNIDLVYGLPRQTF
                     ERLDRTLSDVLKLAPDRIAVFGYAHLPERLTHQRLIDSTTVPGAVERHAFAAHIADRI
                     EAGGYQRIGLDHFARQHDALAKQPVKRNFQGYTIDAADALIGLGASAIGRLPQGYVQN
                     ATATGDYQRRVHETGLAAVRGIALSADDRLRASVIERLMCSFELKTSELEREFGSSAR
                     PILAEAEALLARDGDGIVERADDGFRITPAGRPFVRSVCAHFDAYLGRGSTRFSTGI"
     gene            2277..2957
                     /locus_tag="PK264_12170"
     CDS             2277..2957
                     /locus_tag="PK264_12170"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD76676.1"
                     /translation="MIDLNAVRAAPVAREPFTYFACRNTLHPADLTRISRDFPRIVGT
                     GIHPLHELTFGPAFQSLVDDLRSAEFGQLMSEKLGVALEGRPLMITVRGYCHRKDGKI
                     HTDSKDKIATILLYLNEQWGNDGANASGCLRLLRSGTDLEDKLAEIPPHGGTMVAFRR
                     SENSYHGFVPCEAKRSDNSWHGHHPFEGQRRYLMCNWLVSDAAHVRHSGRHTLSSKIK
                     RLDPRTWF"
     gene            2966..3616
                     /locus_tag="PK264_12175"
     CDS             2966..3616
                     /locus_tag="PK264_12175"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD76677.1"
                     /translation="MDQLIQPNATARAFLAALAAAEHVTRPYDYWLFENALPGDICDG
                     IASLPYAPPAAPIHSGRRESNNSTRVYFSPEVQAEHRVCREVADAFRHPMVVRAIEAR
                     TRCRAGDGHLRIEYCQDVDGFWLEPHVDIPVKLFTMLIYLSRDPALADAGTDVYDASD
                     KHTLVDSAPYRWNSGMIFIPGKDTWHGFTPRPIRGVRKSIIVNYVSSEWRAKHELA"
     gene            complement(3642..4625)
                     /locus_tag="PK264_12180"
     CDS             complement(3642..4625)
                     /locus_tag="PK264_12180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HRD76678.1"
                     /translation="MDKFKALVVRKADKAQTVSMEEFGPADLMEGDVTVRVSHSTINY
                     KDGLALTGKAPVVRRFPMIPGIDFAGTVTASSHPRWKAGDEVVLNGWGCGETHLGGYA
                     SVARVKGDWLVRRPAAFTPAQTMAIGTAGYTAMLAVLAIEAQGVTPASGPVLVTGAAG
                     GVGSVAIAVFSRLGYEVIASTGRMEEGDYLKGLGAKEIINRADLAGPAKPLGRERWAG
                     AVDAVGSHTLANVLSMTRYGGAVAACGLAQGMDLPSSVAPFILRGVSLHGIDSVMAPI
                     AKREIAWARLAKDLDVKKLDAMTVARPLADAPSLGGEILAGKIRGRVVLTV"
     gene            4853..>5258
                     /locus_tag="PK264_12185"
     CDS             4853..>5258
                     /locus_tag="PK264_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HRD76679.1"
                     /translation="MKICMIGAGYVGLVSAVCFAEFGWTVACIDKDRVRVDGLKSGKV
                     PIFEPGLDEVLARTLKSGRLSFSSELGPAVSEADIIFLAVGTPMRRGDGYADLTFVYK
                     AVEDLAPHLKNFTVVTTKSTVPVGTSREIARRL"
     assembly_gap    5259..5406
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5407..6179
                     /locus_tag="PK264_12190"
     CDS             <5407..6179
                     /locus_tag="PK264_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRD76680.1"
                     /translation="KPLALRNAPVMFVSPESAELAKYAANAFLAMKISFINEVADLCE
                     KTGADVQQVATAIGKDRRIGDKFLHPGPGYGGSCFPKDVSALIRIGREAKAPISLVEQ
                     VQRVNDERKIAMAHRVIEALGGTIDGKIIAVLGVTFKPNTDDMRDAPSLTIVPMLQER
                     GAKVRVYDPQGRQHGEELLAGVTWSANATDAADGADGLVVITEWNEFRAIDLKSLAKR
                     MRGRILIDLRNIFQSREARAAGLAYIGIGRRPSEAQTA"
     gene            6765..7112
                     /locus_tag="PK264_12195"
     CDS             6765..7112
                     /locus_tag="PK264_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD76681.1"
                     /translation="MINHAFMPADRSADALAGWKTALQRAASAPQVTIKISGIGLRGR
                     PWSLDDNRRIILEAIDAFGPSRAMFASNFPVDGLCGSFDAIYSGFLVATSGLALADRQ
                     SLFAGTAKRVYRL"
     gene            complement(7155..7349)
                     /locus_tag="PK264_12200"
     CDS             complement(7155..7349)
                     /locus_tag="PK264_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HRD76682.1"
                     /translation="MRSVLRIAALAAAAGLMLGGCGVRGALEAPPEAKKDAEEAKAAR
                     SKDPNYKPEHKGFILDGLLR"
     gene            complement(7438..>7719)
                     /locus_tag="PK264_12205"
     CDS             complement(7438..>7719)
                     /locus_tag="PK264_12205"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018804.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRD76683.1"
                     /translation="DLADWLVRTLGMPFRDAHHVTGAVVKLAESKGLDLEQVPLSEMQ
                     AIEPRITKDVFTVLSVEASVTSRTSFGGTAPQSVGKMAGTWIKRLETAK"
     assembly_gap    7720..7759
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7760..8812)
                     /gene="argH"
                     /locus_tag="PK264_12210"
                     /pseudo
     CDS             complement(7760..8812)
                     /gene="argH"
                     /locus_tag="PK264_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491650.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            8928..9563
                     /locus_tag="PK264_12215"
     CDS             8928..9563
                     /locus_tag="PK264_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRD76684.1"
                     /translation="MSSPDMNSGSGSRLCIVVAAAAAFMLIGFAAVYVTFRPADNAAP
                     VKAAAPPATTSDKLNRGEMATFVFKKAAEDLPAVTFQDAAGKERTLADWKGKVVLLNL
                     WATWCAPCRKEMPGLDRLQKALGSDKFEVVALAVDRAGPEKAKKFLEDTKVEKLALFI
                     DATARSGAALRAVGMPTTILIDASGREIGRLVGPTEWDSEDALRLIKAQLK"
     gene            complement(9577..10545)
                     /locus_tag="PK264_12220"
     CDS             complement(9577..10545)
                     /locus_tag="PK264_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76685.1"
                     /translation="MTKRTAVIGAGMAGLACARVLRRAGCYVEVFDKERIIGGRISTA
                     RLGVHAFDTGAQYITARSDVFKTYIDEVVGTGYASRWQPKFSNGHVGNQLTPWYVGTP
                     GMHSLVRPLTESVRLHTERTVHTIQRSEKGWHLWFEDETSAGPFHAVAVAIPAAQAQL
                     LLGRIDALANPLSRVRMSPCWSLMARLDDRLLPDQDVYSDMSEVIRWICRNNAKPGRQ
                     PRGDQIIVHASQSWSRETEDADPEAVADELWAEVGNVLGLPPARPTQMAATLWRHGLV
                     DQSLGETFLFSSEHMVGVAGDWCLGRLAEHAYESGVSLGRAIVNSL"
     regulatory      10735..10831
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11033..12184
                     /gene="gcvT"
                     /locus_tag="PK264_12225"
     CDS             11033..12184
                     /gene="gcvT"
                     /locus_tag="PK264_12225"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRD76686.1"
                     /translation="MTDNSGSPLRRTPLYDEHVRLGAKMVPFAGYEMPVQYPLGVLKE
                     HLWTRDKAGLFDVSHMGIAYVHAADHRHETAARALEALCPADFVNLAPGQQRYSQLLA
                     ADGGILDDLMVARPVDPAHAGTLCLVVNAGCKVADYAHIAAHLPSGVTLAPSDDFALL
                     ALQGPAAVAVLSDHDRRVAGMGFMTTGAASIAGVDVGLSRSGYTGEDGCEIFVPVEKA
                     AGFWRSLAGDARVQPIGLGARDSLRLEAGLCLYGHDIDTTTSPIEAGLAWSIQKRRRV
                     EGGFPGAARVQRELRDGPSRLRVGIRPDGRAPAREGTEIRAKDGRVIGKITSGGFGPT
                     ANAPVAMGYVERASAALGTYIDLMVRGKPLPATIAAMPFTPHRYARKTT"
     gene            12203..12580
                     /gene="gcvH"
                     /locus_tag="PK264_12230"
     CDS             12203..12580
                     /gene="gcvH"
                     /locus_tag="PK264_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRD76687.1"
                     /translation="MAKDYFTKDHEWIRVEGDVGTVGITAYATGQLGDVVFVEVPEVG
                     RKVKAHEACAVVESVKAASDIYAPVSGEVVDVNGELTTTPAMVNESPEARAWFYKLKI
                     ADPKELAGLMDKATYDAHCETGA"
     gene            12586..>13078
                     /locus_tag="PK264_12235"
     CDS             12586..>13078
                     /locus_tag="PK264_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRD76688.1"
                     /translation="MRYLPLTDADRASMLAKIGVKAVDDLFVAVPPGKLMTALPNLPK
                     AKGEMEVEREMGRLASRNTPAGSVPFFVGGGAYRHHVPASVDHLIQRSEFLTSYTPYQ
                     PEITQGTLQYLFEFQTQVALLTGMDVANASMYDGSTSAAEAVLMAHRVTRRKKALLSS
                     GLHP"
BASE COUNT         2146 a         4311 c         4226 g         2207 t
ORIGIN      
        1 caaggccggc ctcaagttct cgatgctcgg cagcgaggcc atgtggtggc tggctgccgc
       61 catcgcactc gtcgtggccc tggtcatcac gctgaagctg acgcgccgcc tcgcgcggcg
      121 ctgaccccag gacccggtcc attgcagacg gtagctggtt tccagaggcg gtcgattcga
      181 agcatcgctc cgcgatggcg accattggcg gggctcgggg cctagccccg accaacctga
      241 ggtatctcgc tcgcttgtga tgcctcagcg cgacttttcg agcttcaatg cgtcgatcgt
      301 ccccgtgttg gggcaatatc gcgcgaggcg ctcggcggcg tcctcctgct cgaggtcgaa
      361 tgcgcagcgc ccaggttgcc cgcatcgact gcatacgcgc tcgaggtcgc gggcgacggc
      421 cggctccgcg gcggcaatgg tcaccttgtc gagcccgatc gcctcgagcc gcctcggcag
      481 cagctcctgc ggtccctcat gattgacgtc gatgcgctcg agatcgaact cgctgagccc
      541 gttctcttgc agcacgcgtg cccgctcgcc cgcgggcagt gcgagcagct cggccttgag
      601 ggcgcgagcc ttgcgccagc gcttccatgc ctcgggaact gcttcggcca tgcgtgtcgc
      661 gagattcgat gacatgtgcc gctctcctcg tgtcgccaat ctgagttcgg gctgatccca
      721 gcatgtcatg gctgccgcgg caccacttga tcgaggtcaa gcgcggatgc ggcagagcgc
      781 gcaaatcttg cgccgtctgc aaccattgga cccggcggcc atgcacaccc cattgacgcc
      841 cgaggccatc gagcgcctcg ccgcgcccgt cccgcgctac acgagctatc cgaccgcgcc
      901 acacttttcc ggcgcgatcg acgcgcgggc ctatgccggg tggctcgaga gcctgccggc
      961 agacgcctcg ctatcgctct atctgcacat tccctactgc cagacgctct gctggtactg
     1021 cggctgcaac accaaggcga cgcagcgcta tcagccggtc tcgacgtacc tccaagcctt
     1081 gaggcgtgag atcggcgccg tggctcgtct gctgccggcg gtacatggtg tggcccacat
     1141 tcatttcggc ggcggctcgc cgaacattgt cggagctgcg gatttcgaaa cgctcgtggc
     1201 ccagctgcgc agggagttca ctgtcgccgc agatgccgag atcgccgtcg agatcgatcc
     1261 gcgtcattgc aatgacggct acgccgcggc gctccagcgt gccggcatca cgcgtgtctc
     1321 tatcggcgtt caggatttcg atcctgaggt gcaggcggcc atcaaccgga tacagagctt
     1381 cgaggcgacg gcgcgcgtgg tgagggatct gcgggcgcac ggcatcggct cgctcaacat
     1441 cgatctcgtc tatggcctgc cgcgccagac gttcgagagg ctcgatcgca ccctatccga
     1501 tgtcctgaag ctcgcgcctg atcgcatcgc cgttttcggc tatgcgcacc tccccgagcg
     1561 cctcacgcac cagcgcctga tcgacagcac gactgtcccg ggtgccgtcg agcggcacgc
     1621 gtttgcagca cacatcgcgg atcgtatcga ggccggcggc taccagcgca tcgggctcga
     1681 tcatttcgcg cgccagcacg atgcgctcgc gaagcaaccg gtgaaacgca attttcaggg
     1741 ttacacgatc gatgccgccg acgcgctgat cgggctcggc gcttcggcga tcggccgcct
     1801 gccgcagggc tatgtgcaga atgcgacggc gacgggcgac taccagcgcc gagtgcatga
     1861 aaccggcctc gccgccgtgc gcggcattgc gctctcggca gacgatcggc tgcgtgcgag
     1921 cgtgatcgag cgattgatgt gctccttcga actcaagacg tccgagctcg agcgtgagtt
     1981 cggatcgagc gcgcgaccaa tcctcgccga ggcggaagcg ttgctggcgc gggacggcga
     2041 cggcatcgtc gagcgcgctg acgacggctt ccgcatcacg cccgcgggcc ggccgttcgt
     2101 gcgctccgtc tgcgcgcatt tcgacgccta cctcggacgc ggcagcacgc gattctccac
     2161 cggaatttga cgtgcctgcg acgatgcggc tcgcaaatct tgacctggat caacgtagag
     2221 cacgggcgaa gcaccgaggc tcgatccact gatggccatc atcgaagggg ccccccgtga
     2281 tcgacctcaa tgctgttcgg gcagcacccg tcgcccgtga gccgttcacc tacttcgcct
     2341 gccgcaacac gctgcaccct gcggacctca ctcggatcag tcgcgatttc ccgcgcatcg
     2401 tcggcacggg catccacccg ctccacgagc tgacgttcgg gcctgctttc cagtctctcg
     2461 tcgacgacct gcgctcggcc gagttcgggc agctgatgtc cgagaagctc ggtgtcgccc
     2521 tcgaaggccg gccgctgatg atcacggtgc gcggctactg ccaccgcaag gacggcaaga
     2581 tccataccga cagcaaggac aagatcgcga ccatcctgct ctacctgaat gagcaatggg
     2641 gcaacgacgg tgccaacgcg agcggctgcc tgaggctgct caggtccggg actgatctcg
     2701 aggacaagct cgccgagatc ccgccgcacg gcggcacgat ggtcgccttc cgccgctcgg
     2761 agaactccta tcacggcttc gttccctgcg aggccaagcg gtccgacaat tcctggcacg
     2821 ggcatcaccc cttcgagggc cagcgccgtt acctgatgtg caactggctc gtgtccgatg
     2881 ccgcccacgt ccgtcacagc ggtcgtcaca ccttgtcgtc gaaaatcaag cggctcgatc
     2941 cgcgcacctg gttttgaggc ccgacatgga ccagttgatc caaccgaatg cgaccgcacg
     3001 agccttcctc gcagctcttg ccgcggccga gcacgtgacc cggccctatg attactggct
     3061 cttcgagaat gcgctgccag gcgacatctg cgacggtatc gcgagcctgc cctacgcgcc
     3121 gcccgcagcg cccatccaca gcgggcgccg cgagagcaac aactcgacgc gggtttattt
     3181 ctcgccggag gttcaggccg agcatcgggt ctgccgagag gtcgccgatg cgttccgcca
     3241 tcccatggtc gtgagggcga tcgaggcgcg cacgcgatgc cgcgcgggcg acgggcacct
     3301 gcgcatcgag tactgccagg atgtcgacgg cttctggctc gagccgcacg tggacatccc
     3361 ggtgaagctc ttcacgatgc tgatctatct ctcgcgcgat cctgccctcg ccgatgccgg
     3421 caccgatgtc tacgacgcga gcgataagca cacgcttgtc gactccgcgc cctaccggtg
     3481 gaactccgga atgatcttca ttccgggcaa ggacacatgg cacgggttta cgccgcgtcc
     3541 gatccgcggc gtgcggaaat cgatcatcgt caactacgtg tcgtccgagt ggcgggcgaa
     3601 gcacgagctc gcctgagccc gcaccttact gcgcgacccg ctcacaccgt cagcacgaca
     3661 cgtccgcgta tctttccggc caggatctcg cccccgagcg agggcgcatc ggcaagcggg
     3721 cgcgccacgg tcatcgcgtc gagcttcttc acgtcgaggt ccttggcgag ccgcgcccag
     3781 gcgatctctc gcttggcgat cggcgccatc acgctgtcga tgccgtggag cgagacgccg
     3841 cgcaggatga agggggcgac gctcgacggc aggtccatac cttgcgcaag gccgcacgcc
     3901 gcgaccgcac cgccgtacct ggtcatgctg agcacattgg ccagcgtgtg gctgccgacc
     3961 gcatccacgg cgcctgccca gcgctcccgc ccgagcggct tggccggccc agcgagatcg
     4021 gcgcgattga tgatctcctt ggcgccgagg cccttaaggt agtcgccttc ctccatccgg
     4081 ccggtcgagg cgatgacctc gtagccgagc cgcgagaaca ctgcgatcgc cacgctgccg
     4141 acgccgccgg ctgcccccgt caccagcact gggccggagg ccggagtaac gccctgcgcc
     4201 tcgatcgcga gcacggccag catggccgta taccctgccg tgccgattgc catcgtctgc
     4261 gccggcgtga aagcggccgg ccggcgcacc agccagtcgc ccttgacgcg cgcgacactc
     4321 gcatagccgc cgagatgcgt ctcgccgcag ccccaaccgt tgagcaccac ttcgtccccc
     4381 gccttccagc gcggatggct tgaagcggtc acagtcccag caaagtcgat ccccgggatc
     4441 atcggaaagc gccgcacgac cggcgctttc cctgtcagtg cgaggccgtc cttgtaattg
     4501 attgtcgaat ggctgacgcg gaccgtgaca tcgccctcca tgagatcggc cgggccgaac
     4561 tcctccatgg agacggtctg ggccttatcc gccttgcgga cgacgagcgc tttgaacttg
     4621 tccattgccg ggctcctcca ggggttcctc cggaattaag ccttccgccg tggcctgtcg
     4681 agcggccgac agcggctgat ccgtgcgtgg gggcggatcg cctcaccgcc atccactgag
     4741 gtgggcgcgc aaatccggtc tctgttgaag ctgacacggc catgggctag ggcctgtggg
     4801 acgacttgcc ggttatgcgg cgcgcatcaa ccagccgaaa cgggagggcc aaatgaagat
     4861 ctgcatgatc ggcgccggct atgtgggcct cgtctcggcg gtctgcttcg ccgagttcgg
     4921 ctggacggtc gcctgcatcg acaaggaccg ggtgcgtgtt gacggcctca agtccggaaa
     4981 ggtgccgatc ttcgagccgg ggctcgacga ggtgctcgcc cgcacgctca agtcgggacg
     5041 gctctcgttc tcgagcgaac tcggacctgc agtgtcggaa gccgacataa tctttctcgc
     5101 cgtcggcacg ccgatgcgcc gcggtgacgg ctacgccgat ctcactttcg tatacaaggc
     5161 ggtcgaggac ctggcgccgc atctaaagaa cttcaccgtc gtgacgacca agtcgaccgt
     5221 cccggtcggc acgagccgtg agatcgcgcg tcgcctgann nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnacaa gccgctggcg ctccgcaatg cgcccgtgat gttcgtatcc cccgaatcgg
     5461 ccgagctcgc gaaatatgcg gcaaacgcct tcctcgccat gaagatctcg ttcatcaacg
     5521 aggtcgccga tctctgcgag aagaccggcg ccgacgtaca gcaggttgcg accgccatcg
     5581 gtaaggaccg gcgcatcggc gacaagttcc tgcatcccgg gcccggctac ggcggttcgt
     5641 gcttcccgaa ggacgtctcg gcgctcatcc gcatcggccg tgaggcgaag gcgccgatct
     5701 cgctcgtcga gcaggtccag cgcgtcaatg acgagcgcaa gatcgccatg gcgcaccgcg
     5761 tgatcgaggc gctgggcggc acgattgacg gcaagatcat cgctgtgctg ggtgtcactt
     5821 tcaagccgaa taccgacgac atgcgcgatg cgccgagcct cacgatcgtg ccgatgctgc
     5881 aggagcgcgg tgccaaggtg cgcgtctacg atccgcaggg ccgccagcac ggtgaggagt
     5941 tgctggctgg cgtgacgtgg tcggcaaacg cgaccgacgc ggctgacggc gccgacgggc
     6001 tcgtcgtcat cacggagtgg aacgagttcc gggcgatcga tcttaagtcg cttgcgaagc
     6061 gcatgcgcgg caggattctc attgatctcc gcaacatctt ccagtcgcgc gaggcgcggg
     6121 ccgcgggcct cgcctatatc ggcatcggcc ggcgaccgag cgaggcgcag acggcatgac
     6181 ccacgcggac atcgagatcg tcgacgcgca tcaccattat tgggacctgt cgctcaaggc
     6241 caatccgtgg ctggtcgaga tgccgcgcat ccccttccgc tacggcgact atggcgacat
     6301 ttgccgcgat ttcctgcccg aagactacga tcgcgcgact gcccgctaca aagtcgccgc
     6361 gaccgtcacc atggaaggcg agtggcggga ggacgacctc gtcggcgaaa gccgctggat
     6421 ggctgatctc aggcattgca ccgggcgtcc cgcggcgcat gtcgcccgca cgttcctgca
     6481 tcgcgtcaca gccgaggcgg agcttgccgc ccaggcgtca ttcccgttcg tgcgaggcat
     6541 ccggcacaag ccgacggcgg cagcccggcc ggacgcgatc gagcgcggtg cggggggcgg
     6601 catgaccgat ccccattggc ggcgcgggta tgcacgtctc gcgggccatg gccttcactt
     6661 tgagctgcag gcaccgtggt ggcacgtgga cgagctcatc gacctgatcg ggcggctcat
     6721 cggcctcctg gggttagcca cgcccggatc ccgagacgcc ggtcgtgatc aatcacgcgt
     6781 tcatgccggc cgatcgaagc gcggatgcgc tggctggctg gaagaccgcg ttgcagcgcg
     6841 ccgcgagcgc gccgcaggtg accatcaaga tttccgggat cggccttcgc gggcggccct
     6901 ggtcgctcga cgacaatcgc cgcatcatcc tcgaggcgat cgacgcattc ggtccgtcgc
     6961 gagcgatgtt cgcatcgaat tttccggtcg acggcctctg cggttcgttc gacgcgatct
     7021 attcgggctt tctggtggct acgtccgggt tagcgctggc ggacaggcag tcattgtttg
     7081 ccggcaccgc gaagcgggtc tatcggctct aggactctac acgcgaaaca gcggtcagcc
     7141 gccggtagcg ctgatcagcg cagcagcccg tcgaggatga agcccttgtg ctcgggcttg
     7201 tagttcggat ctttggagcg ggcggctttg gcctcctccg cgtccttctt ggcctcgggc
     7261 ggagcctcga gcgcgccacg cacgccgcag ccgccgagca tgagcccagc tgccgctgcc
     7321 agcgctgcaa tgcgcaatac cgatctcatc gaggcaagcc ctgtaaggtt cgtcgctcac
     7381 tcaattttcg agccgagcga tagtccaaca cacgaatttg gaggaagtct agcccgatca
     7441 cttcgctgtc tccaagcgct tgatccaggt tccggccatt ttgccgacgc tctgtggcgc
     7501 ggttccacca aagctggtgc ggcttgtgac cgaggcctcg acggaaagaa ccgtgaacac
     7561 gtccttcgtg atgcgcggct cgatcgcctg catctcggag agtggcacct gctcgagatc
     7621 gaggcctttg ctctcggcga gctttacaac ggcgcccgtc acatggtgcg cgtcgcggaa
     7681 cggcatgccg agcgtgcgca ccagccagtc ggcgaggtcn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnc gcgctcttgg gctccatgac ctccaccata cgcgtcattg
     7801 ccgtgagcgc gagggccagc gcatcgagcg cgtcgaaggt gacctccttg tcctcctgca
     7861 tgtccttcga ataggtgagc ggcagcccct tcatgacgag aacgaggctc tggaacgcgc
     7921 cggcgatgcg gccgaccttc gcgcgcacga gttcggccgc gtccgggttc cgcttttgcg
     7981 gcatgatcga ggagccggtc gtcaggctgt cagggagctt gatgaagccg aactgcggcg
     8041 tcatccagag cacgatctcc tcggcgaagc gggaaaggtg cacggccgtg atggtggccg
     8101 cggccagcgt ctccatcacg aaatcgcggt ccgcgaccga atcgagcgag tttgccgtcg
     8161 ggcggtcgaa gccgagatcg tgcgccgtcg cctcgcgatc gatcgggaac gatgtaccgg
     8221 cgagcgccgc tgcgccgagc gggcactcgt tgagccggcg gcgggcgtcg gcaaagcggc
     8281 tgcggtcacg cgccaacatc tcgacgtagg cgaggcaatg gtgtccgaat gtcacgggtt
     8341 gcgccggctg gagatgcgtg aagcccggca tcacgacggc ggcgtgggtc tcggccttcc
     8401 gggcaagcgc cagcatgagg gcggcgatcg cgccatcgaa gccgtcgatg cgatcacgaa
     8461 cccagagccg gaaatcgagc gccacctgat cgtttcgcga gcgggccgtg tgcagacggc
     8521 cggcgggcga gccgatcagg tccttgaggc ggctttcgac gttggtgtga acgtcctcga
     8581 gggctcgcga gaatgtgaac gcgcctccct ccatctccgc gcgcaccttt gcgaggccct
     8641 cgagaatggc cttgccatcg ccatccgaga cgatgccctg ccgcgcgagc atgcgggcat
     8701 gggcctcgga acctcgaatg tcctgtgagg ccagaacctt gtcgaagccg atcgaggcgt
     8761 ttatctcctc catgatggcg gcaggcggtg cattgaagcg tccgccccac attcgattgg
     8821 tcggtttgtc tgtcacgatc tcgtccttgc ggctctcggt ttgcggcact ggcaggggtc
     8881 gaatttcacg gtcacggcga cacccccgtc gtcatgaggt cccgcacatg agctccccgg
     8941 atatgaattc aggcagcggc tctcgcctct gtatcgtcgt ggccgctgct gcggccttta
     9001 tgctgatcgg cttcgcagcg gtatacgtca cgttccgccc cgctgacaac gccgcgccgg
     9061 tcaaggcggc ggcccccccg gcaaccacct ccgacaagct caatcgcggt gagatggcga
     9121 cctttgtgtt caagaaggct gcagaggatc tccccgcagt gaccttccag gacgcggccg
     9181 gcaaggagcg cacgctcgcc gactggaagg gaaaggtcgt gctgctcaac ctttgggcga
     9241 catggtgcgc gccatgtagg aaggagatgc cgggcctcga caggctgcaa aaggcgctgg
     9301 gatcggacaa attcgaggtg gtggcgctgg ccgttgaccg ggccggaccg gagaaggcga
     9361 agaagttcct cgaagacacc aaggtcgaga agctcgctct cttcatcgac gcaaccgcgc
     9421 gctcaggcgc ggcgctgcgc gcagtcggca tgcccacgac gatcctgatc gatgcgagcg
     9481 gccgcgagat cggccgcctg gtcggcccaa ccgaatggga cagcgaggac gccttgcgcc
     9541 tcatcaaggc gcaactgaaa taggatttcg agcgagctat agcgaattga ctatggcgcg
     9601 gccgagggag acgccgctct cgtaggcgtg ctcggcgagg cggccgaggc accagtctcc
     9661 ggcgacgccg accatgtgct cggaagagaa caggaaggtt tcaccgagcg attgatcgac
     9721 gaggccgtgg cgccagagcg tcgccgccat ctgcgtcggg cgagcgggcg gcaggccgag
     9781 cacgttgccg acctcggccc acagctcgtc cgcgaccgct tccgggtcgg cgtcctccgt
     9841 ctcgcggctc cacgactggg acgcgtgcac gatgatctgg tcgccgcgcg gctgccgtcc
     9901 gggctttgca ttgttgcggc agatccagcg gatcacctcg gacatgtccg aatagacgtc
     9961 ctggtcgggc aagaggcggt cgtcgaggcg cgccatcagc gaccagcacg gcgacatgcg
    10021 cacgcgcgag agggggttgg cgagcgcgtc gatgcgcccg agcagcagct gcgcctgcgc
    10081 cgcggggatc gcgaccgcga ccgcatggaa cgggccggcc gacgtctcgt cctcgaacca
    10141 gaggtgccag cccttctcgc tgcgctggat cgtgtggacg gtgcgttcgg tgtggaggcg
    10201 gacactttcg gtgagagggc ggacgaggga gtgcatcccc ggcgtgccga cgtaccaggg
    10261 ggtcagctgg ttgccgacgt ggccgttcga gaatttgggc tgccagcgcg aagcatagcc
    10321 ggtgccgacg acctcgtcga tgtaggtctt gaagacgtcg ctgcgcgccg tgatgtactg
    10381 ggcgccagtg tcgaaggcat ggacgccaag ccgggcggtc gaaatgcggc cgccgatgat
    10441 gcgctccttg tcaaaaacct cgacatagca gccggcacgg cgcaagacgc gcgcgcaggc
    10501 aaggcccgcc attcccgcgc cgatgactgc cgtccgcttg gtcaaaacac gctccttggg
    10561 ccgtgcacga tgacgctgct ctggcccgct ccatgcccga ccttagtgcg atagccctaa
    10621 tttcgagttt accctgagta attgtgctgt gcccccatgc gaagcggtcg acaacgaagc
    10681 gttcacgatc gttgacttcg agggggattg ccttaaggtt gcggccttcg ccagtcgacg
    10741 cctgcggaag agagccgtgc cagtgttcgg cgcggccgcc gaaggagcaa ccgccccgga
    10801 aactctcagg cagaaggacc gcaggtcgat ggcactctgg aaagcagccg gcgctcgcgc
    10861 gcctggctca ccgaaggggt aaggtgcggc ctcggttgcc ggacggtggc cggatcgccc
    10921 gaaatctctc aggtcccgtg acagaggggg cttcactctg cgtcccgatg ggacgcggcg
    10981 ccgaagccgc ttcgaacgtc agacattcgc acgtcagaca cacgggaccc tgatgaccga
    11041 caactccggt tcacccctcc gcaggacgcc gctctacgat gagcacgtgc ggctcggcgc
    11101 caagatggtg ccgttcgccg gctacgagat gccggtccag tatccgctcg gcgtgctgaa
    11161 ggagcacctg tggacccgcg acaaggcggg gctcttcgac gtgagccaca tgggcatcgc
    11221 ctacgtgcat gcggcagacc accgccacga gacggccgcg cgagcactcg aggcgctctg
    11281 cccggctgat ttcgtcaatc tggcgccggg tcagcagcgc tacagccagc tgctcgcggc
    11341 cgacggcggc attctcgatg acctcatggt ggcccggccg gtcgatccgg cccacgccgg
    11401 gaccctttgc ctcgtggtca atgccggctg caaggtggcc gactatgccc atatcgcggc
    11461 gcaccttccg agcggtgtga cgctcgcgcc gtccgacgat ttcgcgcttc tggcgctgca
    11521 gggacccgcg gcagttgccg tgctttccga tcatgaccgc cgggtcgcag gcatgggctt
    11581 catgaccacc ggagcggcct cgatcgccgg cgtcgatgtc ggcctctcgc ggtccggcta
    11641 cacgggcgag gatggctgtg agatcttcgt gccggtggag aaggcggccg gcttctggag
    11701 gtcgctcgct ggagacgcgc gggtccagcc gatcggcctc ggggcccgcg attcgctccg
    11761 cctggaggcg ggcctctgcc tttatggcca cgacatcgac acgaccacgt cgccgatcga
    11821 ggcgggcctc gcctggtcga tccagaagcg gcggcgtgtc gagggcggct tcccgggagc
    11881 ggcgcgcgtc cagcgagagc ttcgtgatgg gccatcgcgc ctgcgcgtcg gtatccggcc
    11941 tgatggccgc gcgccggccc gcgagggcac cgagatccgc gccaaggatg gtcgcgtcat
    12001 cggcaagatc acgtcgggcg gcttcggtcc cactgcgaac gcgccggtcg ccatgggcta
    12061 cgtcgagcga gcctccgccg cgctcggcac atacattgat ctcatggtgc gggggaagcc
    12121 gctgcctgca accattgccg cgatgccgtt cacgccgcat cgctacgcac gcaagacaac
    12181 ctgaacaacg agggaggcag acatggccaa ggactatttc acgaaggacc acgagtggat
    12241 tcgcgtcgaa ggtgacgtcg gcacggtcgg catcacggct tatgccaccg gccagctcgg
    12301 cgacgtcgtg ttcgtcgagg tgccggaggt cggccgcaag gtgaaggctc acgaagcgtg
    12361 cgccgtggtc gagagtgtca aggcggcgag cgacatctat gcgcccgtct cgggcgaagt
    12421 cgtcgatgtc aacggcgaac tcaccacgac acccgccatg gtgaacgaga gccccgaggc
    12481 ccgcgcctgg ttctacaagc tgaagatcgc cgatccgaag gaactcgccg gcctcatgga
    12541 caaggcgacc tacgacgccc attgcgagac gggggcgtga ggggcatgcg ctatctccca
    12601 ctgacggatg ccgatcgcgc gagcatgctc gccaagatcg gggtgaaggc ggtcgacgac
    12661 ctgttcgtcg ccgtgccgcc tggcaagttg atgacggcgc tccccaacct gccgaaagcc
    12721 aagggcgaga tggaggtcga gcgcgagatg gggcggctcg cgtcccgcaa cacgccggcc
    12781 ggcagcgtcc ccttcttcgt tggtggtggc gcctaccgcc accatgtgcc ggcgagcgtc
    12841 gaccacctga tccagcgctc cgagttcctg acgagctata cgccttatca gcccgagatc
    12901 acccagggca cgctgcaata tctgttcgag ttccagacac aggtcgcact gctcaccggc
    12961 atggatgtcg ccaacgcctc gatgtacgac ggctcgacct cggccgcaga agccgtgctg
    13021 atggcgcatc gcgtaactcg gcggaagaag gcgctgctct ccagcgggct ccaccccc
//
LOCUS       DAORNH010000108        13033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3616_length_13033_cov_2.716366, whole genome shotgun sequence.
ACCESSION   DAORNH010000108 DAORNH010000000
VERSION     DAORNH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13033
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_13033_cov_2.716366"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..601)
                     /locus_tag="PK264_12240"
                     /pseudo
     CDS             complement(1..601)
                     /locus_tag="PK264_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008046.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
     gene            complement(979..1527)
                     /locus_tag="PK264_12245"
                     /pseudo
     CDS             complement(979..1527)
                     /locus_tag="PK264_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
     gene            1856..2806
                     /locus_tag="PK264_12250"
     CDS             1856..2806
                     /locus_tag="PK264_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76689.1"
                     /translation="MAAARRSGSVDISTCSGRPWLELISRLAASLLLIASMLPNLVYM
                     AGRDEATAAARRAGGASETSTRVGEYHIGGYLGAPHTHPSDVYIRKPPGTEVLIKDVP
                     WEGRPFRHPIYYGIRGLAWRSDAFAAMLDFTHSKTIPAKDARLTLSGTRDGKAVPKSA
                     TVGETFRHFEFSHGHNMLAASAVLRMPAVMPRVRPYFGAGPGVNLPHTEVQFIDDPVR
                     TYMYQLTGLMAQALVGLEVELPGGTVFLEYKFTFSKYDAPLMNVTTTLAPTDLYQQLR
                     RWMAGRAPEGGWLETMLASHQVVAGMTGRVAGGHRMLGSR"
     gene            2931..3455
                     /locus_tag="PK264_12255"
     CDS             2931..3455
                     /locus_tag="PK264_12255"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD76690.1"
                     /translation="MQDTGAGADAPPADAVMRPLAGPIAMLAERIALIGGLISLSVAG
                     LVVTSVLGRWLFAQPVTGDFDLVQMGTAVSVFAFLPLAQARRSNIMVDTFTSWLPYRV
                     QAAIDVIWDIVYAGMAALLTYCLVNGTIGEWRTGTTAQMIALPTWPAVAACALMCAIL
                     AIVALATACARRRP"
     gene            3452..4969
                     /locus_tag="PK264_12260"
     CDS             3452..4969
                     /locus_tag="PK264_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD76691.1"
                     /translation="MSGTAAALVGFGAMLALIAIRMPVGLAMLVTGSIGFISYTSLPA
                     YLSFMKTRPYTEFASFTLSVIPLFILMGALAEKSGLSTALFNAAAQAFARVRGGLAMA
                     VIGACTAFGAICGSSVATTATFGRAALPELLRFGYNGGFATGTIAVGGTLGILIPPSV
                     ILVVYAITTEQNIAKLFMAALIPGLLAATFYCIAIALIARASPEKAPLVETRSVGGRH
                     LALSDMLVALTGVATIAAVLWFKPVPRLLLARLPLGPLGTFEIALSTGLVALAAVVLL
                     LAWLARRSDPLRSVLPVIAIAFVVVGGIYGGVFTPTEGAAVGAIAMLLLGLVQGTLDW
                     PKIRGSLLQTAETTGMVFIILLGADLFDAFLARTQMPEHAAEWITGLGLSPYTVLVAL
                     LVFYIVLGAVMDELAMLLLTLPVFFPIIQKLNFGLPPEEVAIWFGILVLIVVGIGLTA
                     PPIGLNVFVISSIARGVPMPETYRGVLPFILMDILRLALVVLFPGLALWLVRLLS"
     gene            4982..5281
                     /locus_tag="PK264_12265"
     CDS             4982..5281
                     /locus_tag="PK264_12265"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD76692.1"
                     /translation="MTRWVAIFEDDPDADWVRKRHDQDHFAYLDEHAGKIRLGGGLRP
                     APGEWFVGGLWVLEVESREEAVRLIEGDPYFQKGLRRRYRLLVWGKAPCYGEVIL"
     gene            5302..6912
                     /gene="ggt"
                     /locus_tag="PK264_12270"
     CDS             5302..6912
                     /gene="ggt"
                     /locus_tag="PK264_12270"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD76693.1"
                     /translation="MTHGMVCAAQPEAVEAGAEVLQNGGNAVDAAIATALVQTAVDPQ
                     MCGIAGFGSLQVYDPAKGRHACLDFYGRAPAAVRPDMWEHLLIGEAEDGFGFLLEGRV
                     NEIGYGAITTPMTLAGFAEALKTYGSGRISLGDLIEPAARYAEGGFNVRPHVHAYWTQ
                     MPTEGRAPHSDFLTKLPATRRIYQKADGRLHGVGELLRNPDMGRTLRRVQTAGVAEFY
                     SGSIAREITADMAANGGLITAADLAGAKPETHAPLQGTYRGLTLTSNHPPGGGVMLVE
                     MLNILEGFDLKGMGHNSADYIATVSEAMKIATVDKDQKVGDPRFVDVPMAELTSKAYA
                     ADKAALIRRGVKTHVPRLKSKESADTTHVTVVDEHGVCASMTHTLGTPSGVVTEGLGF
                     MYNGAMAVFDPRPGRAGSLAPGKSRFSSMAPTIIFKDGKPMLVLGAPGGTYIAMGILQ
                     VILNVVEFGMSALEAVSAARFCATSDTIQLTNRILRSTEAELVRRGYPVRRWPVNYHF
                     AGVHAIRIMDGKLDGGADPGRDGMALAV"
     gene            7485..>7711
                     /locus_tag="PK264_12275"
     CDS             7485..>7711
                     /locus_tag="PK264_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRD76694.1"
                     /translation="MDLRAIEAQGIRPDEIASLDVFEIAGTIKWFDISKGYGFIVPDN
                     GLPDVLLHVTCLRAGGYPTAYEGARVHAQVLR"
     assembly_gap    7712..7753
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7754..8056
                     /locus_tag="PK264_12280"
     CDS             <7754..8056
                     /locus_tag="PK264_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRD76695.1"
                     /translation="ESTATHPSQLPQRTHVVVEAESDWERAMVKWFNRVRGFGFLTRG
                     DNTPDIFVHMETLRRHGFAELRPGQIVQVRWGMGLKGCMAADLRPDGAPSNVHNAH"
     assembly_gap    8071..8080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8088..8579
                     /locus_tag="PK264_12285"
     CDS             8088..8579
                     /locus_tag="PK264_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRD76696.1"
                     /translation="MRSSPKAARCAALVALVALLASLLAVTGVDAEMRRESLTLVTAK
                     GERLINIEVAETSEEKSLGLMFRTSLADDAGMLFPYAPAQEIQMWMRNTYIPLDMVFI
                     RADGVIHRIEVRTEPLSERVISSDGLVAGVLELAGGNAERLGLRPGDRVVHPHFKGKP
                     KGR"
     gene            8628..9947
                     /locus_tag="PK264_12290"
     CDS             8628..9947
                     /locus_tag="PK264_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRD76697.1"
                     /translation="MSDLFSAAGLADAVARPLADRLRPARLADVSGQDHLVGPEGTLT
                     TMLKAGRLPSLVLWGPPGCGKTTIARLIAAETRLELEQMSAIFSGVQDLRKAFERARA
                     RRETGRGTLLFIDEIHRFNRAQQDSFLPLMEDGTITLIGATTENPSFELNAALLSRAI
                     VLTLNRLGPAALEALLARAEGVMKRRLPLVPDAREALLAMADGDGRSLLNMAEEVFAA
                     VTGDRAPLDRAALTHIVQRRAPIYDKSREGHYNLISALHKSVRGSDPDAALYWLARML
                     DGGEDRLFLARRLVRMAVEDIGLADPQALVQAIAAKDTYDFLGSPEGELALAQVTVYL
                     ATAPKSNGVYVAYKAAMRAAKESGSVAPPKHILNAPTELMANEGYGAGYAYDHDAEDG
                     FSGQNYFPESFAARPTYYDPPERGFEREIRKRLAYWEGLRRKRNEGR"
     gene            10560..10823
                     /locus_tag="PK264_12295"
     CDS             10560..10823
                     /locus_tag="PK264_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76698.1"
                     /translation="MRPGLQFGFLGVVPAQTNRPFEDAEQLPAQQSPLTEQVAPAGLH
                     FAWAGSAPNVATVPRATTLAQMMPNTRFGIFRNFMPIFLHGFL"
     gene            complement(12505..>13033)
                     /gene="yihA"
                     /locus_tag="PK264_12300"
     CDS             complement(12505..>13033)
                     /gene="yihA"
                     /locus_tag="PK264_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRD76699.1"
                     /translation="VAFAGRSNVGKSSLINALVRQKGLARTSNTPGRTQELNFFVADE
                     VALAIVDLPGYGFAEAPKEKVKAWTGLIKDYLRGRQALARVYLLIDSRHGVKSVDEEI
                     MALLDEAAVSYQIVLTKIDKIAPKALDAVLAATRAAIARHAAAFPEIIATSSETGAGI
                     DALRDAAAQLFGEMR"
BASE COUNT         2164 a         4271 c         4261 g         2285 t
ORIGIN      
        1 cgcttggcga tctcctgtcc cttgacgtcg gcgagatcgg cgagcggtcc cgccttgtcg
       61 tcgagatctg ggacgggcgg cgccagaacc tgcaatccct tccagtggtt gatgagggcc
      121 aggaggtggg gtggcgccaa caccgacatc tgatcgccgg cccaggccgc ctcgggacct
      181 gtgtcagcgg gacagacgag acctttcccc atggcgtggg cgccgatggc ggccggcaag
      241 ccgccaggca cggggcggat cgaaccgtcg agcgccaact cgcgatcgcc gcgtagccct
      301 cgacggcatc gggggcgagc gcacccatag cgaccatcag cgcgagcgcg atcggcaggt
      361 cgaaatggct cccttccttc ggaatgtcgg ccggcgagag attgaccgtg atgtgcttga
      421 acggcagccc cagcccgacg gcatgcagcg cattgcgcac gcgctcgcgg ctttccgcca
      481 cggccttgtc cggcaggcca acaatcgcga aatgcggctt gcccccgctg atgcggacct
      541 gcacgtcgat cggccgtgcc tcgatcccct ggaatgcaat cgtcgcgatc tgcccgtaca
      601 tctgcccctc ggaccgttac gctgagcggg gtctgcaaat aagcacacat ttcggatcaa
      661 aacaagaaca ttaatcgaac caacgcgggt catcgcatgt ggttcggcct ccgggccagc
      721 gccagcgcga gcaggcgggc atcgccgccg atcagcgcgc gctcgacggc aagcgcgcgg
      781 cgtacgccac gtgccagcgc gacgccgacg cgcgccgcga tcgccgccga tccaggggtg
      841 gccgtcgtcg atcttgcggg cgagcagcgg cgccgtagtt gtgaagcggc ggacatagag
      901 gtgataccag ccggatccgg gctccgcctt cggcgcaaca gggacgccgg cgaggcgctc
      961 gaagaggccc ggatcgcgga cgccctcgag tgtgaccgcg gcgaggcggt gcacggcgcc
     1021 atcgccgagg ctgtaccagt cttcgccgcg cgcggcgccg agctcggcca gcatcacgac
     1081 gggcatgacc gcgaagccgt gatagtggag ggcgcgtgcg ccgcgcgaga gttccttggc
     1141 gagcgtgcca tctggtccta tgtcgcgtat ggcgtcggcc atgatcgccc gtgccgcgct
     1201 ccagagcgcc tcgtcgtcgg tgccgagggc ggtcgcggca acggcgaggc ccgtccagta
     1261 ccaatggttg ttgcgctggt tgcgtcggtc gtcgaagaag gcgagtgagc ccgcggcgag
     1321 tgaccggagc cagggctcga tcgcgcggcg ctgaggctcg ctcgcgctgt cacgcacctt
     1381 gagccaggcg agcgcgtagc ccgcgaggtt ccacttgcgc tcgtactcgg cctgcgcgct
     1441 ctgcatctgc ccggtccagg cgccggcccg ggcccaggcc gctagccacg agcgagcgca
     1501 ctcaccggac gcctcgctgc cgcgcatggc tcggtcggca tcgtcgacga ccctttgcag
     1561 gaacacgcgg aaaggctcca tggcggcatc gcgccggcgc cgcttctcgg catcgatgct
     1621 ggatcccttg gcgtcggcgt agaagacgtc gacttcgatc gggtagggcg gcttggccgg
     1681 gcccgggcag tcggacgcct ggacgagggg cgtcgggctc aggactagca cgagggcgag
     1741 gactgctgtg cgcgcgtgcc cgaagaggcg cgacatcgtc gcggcactcc tgccgttacg
     1801 gtcagcgcgg cactcgacgg gagacagtgt tggcgcgagt gccgaggagg cgggaatggc
     1861 agcggcgcgc cgaagcggat cggtcgacat cagcacatgc agcgggcgtc cttggctcga
     1921 gctcatttcg cgcttggcgg cctccctgct gctcatcgcg agcatgctgc ccaatctagt
     1981 atacatggcg ggccgtgacg aggcgaccgc tgcagcccgc cgagcgggcg gggcgtcgga
     2041 gacgagtacg cgcgtcggcg agtatcacat cggcggctat ctcggcgcgc cccatacgca
     2101 tccgagcgat gtctacatca ggaagccgcc cggcaccgag gtgctcatca aggatgtacc
     2161 ctgggaaggg cgcccattcc gccacccgat ctactacggc atccgcgggc tcgcctggcg
     2221 cagcgacgcg ttcgccgcca tgctcgactt cacgcattcg aagacgattc cggccaagga
     2281 cgcgcgactg accctttcgg ggacgcgcga cggcaaggcg gttccaaaat ccgcaactgt
     2341 cggcgagacg ttccgccatt tcgagttcag ccacgggcac aatatgctgg cggcgagcgc
     2401 cgtgctccgt atgccagcag tcatgccgcg tgtacgtccc tacttcggcg ccggtcctgg
     2461 cgtgaacctc ccgcacaccg aggtgcagtt catcgacgat cccgtccgca cctacatgta
     2521 ccagctgacc ggcctcatgg cgcaggcgct ggtcggcctc gaggtcgagc tgccgggcgg
     2581 caccgtgttt ctcgagtaca agttcacctt ctcgaaatac gacgcgccat tgatgaatgt
     2641 gacgaccacg ttggccccga ccgatctcta ccagcagctg cggcgttgga tggcgggccg
     2701 tgcgcccgag ggcggctggc tcgagacgat gctcgcgagc caccaggtcg tcgccggcat
     2761 gacagggagg gtcgccggcg ggcatcgcat gctcggctcg cgatagggtc gaggcaccct
     2821 cggggaccgg cacaatggca acagcagtgt tgtgaagtag tggcgacacg tgccatcgtt
     2881 gccgtgcgtc gaccggagcg ctcgaaccgc cggcacgtgg agggatgccc atgcaggaca
     2941 ccggggcagg cgcggacgcg ccgccggccg atgccgtcat gcgcccgctg gcgggcccga
     3001 tcgcgatgct cgccgagagg atcgcgctga tcggcggcct gatctcgctc tccgtcgcag
     3061 gcctcgtcgt gacgagcgtg ctcggccgct ggctgttcgc gcagccggtg accggcgact
     3121 tcgacctcgt gcagatgggg acggcggtct cggtgttcgc cttcctgccg ctcgcccagg
     3181 cgcggcgcag caacatcatg gtcgacacgt tcacctcgtg gcttccgtac cgtgtgcagg
     3241 cggcgatcga tgtcatctgg gatatcgtct acgctggcat ggcggcgctg ctgacctact
     3301 gcctcgtcaa tggcaccatc ggcgaatggc ggacgggcac gacggcgcaa atgatcgcct
     3361 tgccgacgtg gcctgcggtc gcggcctgtg ccctgatgtg cgcgatcctt gcgattgtcg
     3421 cgctggcgac ggcctgcgcc cggcggcggc catgagcggc accgcggcgg ccctcgtcgg
     3481 cttcggcgcc atgctggcac tcattgcgat ccgcatgccg gtcggcctcg ccatgctggt
     3541 gacgggcagc atcggcttca tctcgtacac gagcttaccg gcctatctct cattcatgaa
     3601 gacgcgaccc tacacggagt tcgcgagctt cacgctgtcg gtgataccgc tcttcatctt
     3661 gatgggggcg ctcgccgaga agtccgggct ctcgacggcc ctcttcaacg cagcggcgca
     3721 ggctttcgcg cgcgttcgag gcgggctcgc catggcggtg atcggggcct gtaccgcgtt
     3781 cggcgcgatc tgcggctcgt ccgtcgcgac gacggcgacg ttcgggcggg cggcgctgcc
     3841 cgagctcctg cgctttggct acaacggcgg ctttgcgacg ggtacgatcg cggtcggcgg
     3901 cacgctcggc atcctgattc cgccctccgt gatcctcgtc gtctacgcga tcacgaccga
     3961 gcagaatatt gccaagctct tcatggcggc gctgatccct ggcctgctcg ccgccacctt
     4021 ctactgcatt gccattgcgc tgatcgcgcg cgcgtcgccg gagaaagcgc cgctcgtcga
     4081 aacccgcagc gtcggcgggc ggcacctcgc gctgagcgat atgctcgtgg cgctgaccgg
     4141 cgtggccacc atcgcggcgg tgctctggtt caagccggtg ccgcgcctgc tcctggcgcg
     4201 ccttccgcta ggcccgctcg gcacgttcga gatcgcgctc agcacgggcc tcgtcgcgct
     4261 cgccgctgtc gtcctgctgc tcgcctggct cgcgcggcgc agcgatcccc tccgctccgt
     4321 gctgcctgtg atcgcgatcg ccttcgtcgt ggtcggcggc atctacggcg gcgtcttcac
     4381 gccaaccgag ggggccgcgg tcggcgccat tgcgatgctg ctgctcgggc tcgtgcaagg
     4441 cacgctcgac tggccgaaga ttcggggaag cctgctgcaa acggccgaga cgacaggcat
     4501 ggtgttcatc atactgctgg gtgcggacct gttcgatgcc ttcctcgccc gcacgcagat
     4561 gcccgagcac gccgccgaat ggatcacagg gctcggcctc tcgccctaca ccgtgctggt
     4621 cgctctgctc gtcttctaca tcgtgctcgg cgcggtgatg gacgagcttg ccatgctgct
     4681 gctgacgctg ccggtcttct tcccgatcat ccagaagctc aatttcggct tgccgcccga
     4741 ggaggtggcg atctggttcg gtatcctcgt gctgatcgtc gtcggcatcg ggctcacggc
     4801 gccgccgatc ggcctcaacg tgttcgtcat ttcctcgatc gcgcgcggtg tgccgatgcc
     4861 cgagacgtat cggggtgtgc tgccgttcat tctgatggac atcctgcggc tggcgctcgt
     4921 cgtgctcttc ccgggcctgg cgctctggct cgtcaggctg ctgagctgaa gggagacggc
     4981 cgtgaccaga tgggttgcaa tattcgagga tgatcccgat gcagattggg tgcgcaagcg
     5041 ccacgaccag gatcatttcg cctacctcga cgagcacgcc ggcaagatca gactcggcgg
     5101 cgggctccga cccgcacccg gcgagtggtt cgtcggaggc ttgtgggtgc tcgaggtcga
     5161 gagccgcgag gaggccgtgc ggctgatcga gggcgacccc tatttccaga agggcctccg
     5221 gaggcgctat cgcctgctcg tctggggcaa ggctccctgc tacggtgagg tcatcttgta
     5281 gctcgagagg cgaggcttcc gatgacccac ggcatggtct gcgcggcgca acccgaggcc
     5341 gtcgaggccg gcgccgaggt gctccagaac ggcggtaacg ccgtcgacgc ggcaatcgcg
     5401 accgcgctcg tacagaccgc tgtcgatccg cagatgtgcg gcatcgcggg cttcggctcg
     5461 ctgcaggtgt acgatccggc aaaaggccgt catgcctgtc tggacttcta tggccgcgcg
     5521 cccgcggcgg tccgtcccga catgtgggag catctgctga tcggcgaggc ggaggatggc
     5581 ttcggcttcc tcctcgaagg ccgcgtcaac gagatcggct atggcgcgat cacgacgccg
     5641 atgacgctcg cgggctttgc cgaggcgctc aagacgtatg gcagcgggcg catatcgctc
     5701 ggcgacctca tcgagccggc ggcccgctac gccgagggcg ggttcaatgt gcgcccgcac
     5761 gtccacgcct actggacgca gatgccgacc gagggccgcg cgccgcattc cgatttcctg
     5821 accaagctcc ccgcgacgcg gcgcatctat cagaaggcgg atggtcgcct gcatggggtc
     5881 ggcgagctcc tcaggaaccc cgacatggga cgcaccttgc gccgtgtgca gacggcgggc
     5941 gttgccgagt tctattcggg cagcatcgcg cgcgagatca cggcagacat ggcggcaaat
     6001 ggcgggctca tcacggcagc cgacctcgcc ggcgcgaagc cagagacgca cgccccgcta
     6061 caaggcacct accgcggcct gacgctcacc tccaaccatc cgccgggcgg cggcgtgatg
     6121 ctcgtcgaga tgctgaacat cctcgagggc ttcgatctca agggcatggg gcacaactcc
     6181 gccgactaca ttgcgacggt ctcggaggcg atgaagatcg cgaccgtcga caaggatcag
     6241 aaggtcggcg atccgcgctt cgtcgacgtc ccgatggccg agctgacctc gaaggcctac
     6301 gcggccgaca aggcggcgct catacgccgt ggcgtgaaga cgcacgtccc acgcctcaaa
     6361 tcgaaggaga gcgcagatac gacccacgtc acggtggtcg acgagcatgg cgtctgcgca
     6421 tcgatgacgc acacgctcgg cacgccgtcg ggcgtcgtca ccgaagggct cggcttcatg
     6481 tacaatggtg ccatggccgt gttcgatccg cgcccaggcc gcgcgggctc gctggcaccg
     6541 gggaagtcgc ggttctcgtc catggcgccg acgatcatct tcaaggacgg caagcccatg
     6601 ctcgtgctcg gcgcgccggg cggcacctac atcgcgatgg gcatcctgca ggttatcctg
     6661 aacgtggtcg agtttggcat gagcgcactc gaggcggtct cggccgcgcg cttctgcgcg
     6721 acctcggaca cgatccagct cacgaaccgc atcctgcgct cgacggaggc cgagctcgtc
     6781 cgacgcggct acccggtccg ccgctggccg gtgaactatc acttcgccgg tgtccacgcg
     6841 attcgcatta tggacggaaa gctcgacggc ggcgccgatc cgggtcgcga tggcatggcg
     6901 ctcgccgtgt gaggatcagc gagtgcggat cgacgcgtgc cccgctcgcc ggggtgcgtt
     6961 gattgccggg cagggtggaa tacgacaacc gtcggttgcg tccgtgttcc tagctggcaa
     7021 ttgaccgacg cgccgagtcg aaactcgctg tcgttgcaga ttcgcaactg ttcgctccgg
     7081 cgcgtcccaa tttcaatgac cgaaaagtgg cggcgagccg gcaccctcgt ggcaacccac
     7141 aggatagtca tagattgggt gtaagcggac ggcgtctcag aatatatttt gaaatcagaa
     7201 ttataaaagt cctgtgctcg caagtgcgat aaaccgcagc agcgcatgcg gcagcgcgaa
     7261 gcggaacaaa agccgttgct cggcaactgg tcagtggtat ctttttatgg tcttcgattc
     7321 gcctcgaatt tgagcggctc ccagagggca aatactgggc atccgcagca agtcagacga
     7381 agcgaagaca gcttatagtg gtaatgttga gcaggggtac tgaaatgggg gactcgaaac
     7441 ttcctccgga gtcatccgga ggcgagcggg ggctcgtgcg cggcctggat ctcagagcga
     7501 tcgaggcaca aggcatcaga ccggacgaga tcgcaagtct cgacgtgttc gagatcgccg
     7561 gcaccatcaa gtggttcgac atttcaaagg gctacggctt tatcgtgcca gacaatgggt
     7621 tgcccgacgt gctcctgcac gtgacctgcc tccgggccgg tggctacccg accgcctatg
     7681 agggtgcacg ggttcacgcc caagtgcttc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnngagagca ccgctaccca tccctcgcag cttccacagc gcacgcacgt
     7801 cgtggtagaa gcggagagcg actgggaacg ggcgatggtc aagtggttca accgcgtgcg
     7861 gggcttcggt ttcctgaccc gcggtgataa cactccggac atcttcgtcc acatggaaac
     7921 gttgaggcgt catggcttcg ctgagcttcg gcctggccag atcgttcagg tccgctgggg
     7981 catgggcctc aagggctgca tggctgctga cctgcgcccg gacggcgcgc cgagcaacgt
     8041 gcacaacgcg cactgagtct ggtcaagcca nnnnnnnnnn tgcgacaatt cgatcttcac
     8101 ccaaggccgc gcgatgcgcg gccttggttg cgttggtggc cctcctcgca agcttgcttg
     8161 ccgtcacggg cgtggatgcc gagatgcgcc gcgaaagcct gacgctcgtc accgcaaaag
     8221 gcgaacgcct gatcaatatc gaggtggcgg agacgtccga ggagaagtcg ctcgggctca
     8281 tgttccgcac gagtctcgcc gacgatgcgg gcatgctgtt cccttacgca cccgcccagg
     8341 aaatccagat gtggatgcgg aacacctaca tcccgctgga catggtgttc attcgtgcgg
     8401 atggcgtgat tcaccgcatc gaggtgcgca cggagccgct ctccgagcgt gtcatctcct
     8461 cggacgggct cgtcgcaggc gtgctggaac ttgccggcgg taacgccgag cggctcggtt
     8521 tgaggcccgg tgatcgcgtc gtccatccgc acttcaaggg caaacccaaa ggacgctgac
     8581 ggtcggctgc ccggcgccct cggctctcgc cacgagaggc aggctcgatg tcagacttgt
     8641 tctcagccgc gggtctcgcc gatgccgtgg ctcgtccact cgccgaccgc ctgagacccg
     8701 cgcggctcgc cgacgtttcg ggtcaggatc atctggtcgg tcccgagggc acgctcacga
     8761 ccatgctgaa ggcaggccgg ctaccgagcc tcgtgctctg gggcccgccc ggttgcggca
     8821 agaccacgat cgctcgcctg atcgccgccg agacgcggct cgagctcgag cagatgtcgg
     8881 cgatcttttc gggcgtgcag gacctccgca aggccttcga gcgggcccgc gcgcgccgcg
     8941 agacggggcg cggcacgctg ctcttcatcg atgaaatcca tcgcttcaac cgcgcccagc
     9001 aggacagctt cctgccgctc atggaggacg gcacgatcac gctgatcggc gccaccaccg
     9061 agaacccgtc cttcgagctc aacgcggcgc tgctctctcg cgcgatcgtg ctgacgctca
     9121 accggctcgg gcccgcggcg ctcgaggcgc tgctcgcccg cgccgagggc gtgatgaagc
     9181 ggcggctgcc tctcgtgccg gacgcccgcg aggcgctgct cgccatggcc gatggtgacg
     9241 ggcgctcact cctcaacatg gccgaggagg tgttcgcggc cgtaacgggc gatcgcgcgc
     9301 ccctcgaccg ggcggcgctc acacatatcg tgcagcggcg cgcgccgatc tacgacaagt
     9361 cgcgcgaggg ccactacaac ctcatcagcg cgttgcacaa atccgtgcgc ggctccgatc
     9421 ccgacgcggc actctattgg ctggcccgca tgctcgacgg cggcgaggac cgcctgttcc
     9481 tcgcgcgccg cctcgtgcgc atggcggtcg aggatatcgg cctcgccgac ccgcaggcgc
     9541 tcgtgcaagc gatcgcggcg aaggacacct acgacttcct gggttcgccc gaaggcgagc
     9601 tcgcgctcgc gcaggtgacc gtctatctcg cgaccgcgcc taagtcgaac ggggtctatg
     9661 tggcctacaa ggcggcgatg cgggcggcga aggagagcgg cagcgtcgcc ccgccgaagc
     9721 acattctgaa cgccccgacc gaactcatgg ccaacgaagg ctatggcgcc ggctacgcct
     9781 acgatcacga cgccgaggat ggcttttccg gccagaacta cttccccgag agcttcgcgg
     9841 cgcgccccac ctactacgac cctcccgagc gcggcttcga gcgcgagatc aggaagcggc
     9901 tcgcctattg ggaggggctc cggcggaaga ggaacgaggg gcggtgacgg gcagggtggg
     9961 cggcgtagcg gcagtggcgg gccgggcgcc gatgtcgcgt gtacttgcac ccatggcgtt
    10021 ggcgtgagaa gtggccgctt tgggccaata gggtgcatgg ccgggacggc gagcactggc
    10081 ttgccgcgcg tccggtccct ggtaggtgag cggaaagaca cgaagacgct gagacggggc
    10141 tcttggccca tgcactaagt cgctgcgcga cagcccgctg atgcgggcgc ttgagccgac
    10201 aggccggaaa ccagttgaga tgacggcggt aacggggccg ggagccccac cctgagcatc
    10261 gaggcgctag caaccctcac tcaggacaga actccccatg accgacacca ccaccatcag
    10321 ccctctagac cattctcaaa ccggcgcaac agggctggct gcttgtgacg ccgggcgcag
    10381 caatagccct gcggcaggca cgcgtgtgcg gctgccgtgt tgagcggcag tcaagacctg
    10441 cccctcgggc caaccgccat ttctattggc cgccggagca gatcaacgcc ccgctgctcg
    10501 gcactttccc gcaataacct gagcaacact cattgttcgg cccttcacgg gagcaggtcg
    10561 tgcgtcccgg tttgcagttc ggattcctcg gcgtagtgcc ggcgcagaca aaccgtcctt
    10621 tcgaagatgc cgagcaattg cctgcgcagc aatccccgct aacagagcag gtcgcgcccg
    10681 ccggtttgca cttcgcctgg gccggttcgg ccccaaatgt tgcgacggtg ccgagggcca
    10741 ccacgctcgc gcagatgatg ccgaacacgc gtttcgggat tttcaggaat ttcatgccaa
    10801 ttttcctcca cgggtttctt taagcttcag actaggatat tcagatcatt gccatctcac
    10861 gccttgcctc cttcccactc ggtaggctgc ctgccaagag agttgggcgc tgtcgcagac
    10921 tgctcaccgc caaaaccacc tctcgacccg cacggctctc atccggccga tcgatgcctc
    10981 atgcgacgat cgccccaaca agcggacgag cttcaagccc gccgtcgcct aagttgaggg
    11041 gataggattg catcctgcgt gagccccaca catacggcac gattatgccg gaactacgaa
    11101 aacaacatgt ctcagcgggc tatcgcgtgg ttatcgcaag gcatttcccg tcgaccgaaa
    11161 cgaagccctt ctcgcacact ctttctcttt tgacggctcc gaccggactg cactttttca
    11221 tgcaagccgc gcgcgatttc gcgcctttgg ctttgtcagg gatattgtca catttttcaa
    11281 agcaactggc ttgcgccacg acaggccccg cgcccaagga gaggacgcag gcgcaggcga
    11341 ttgccgttac gataaacagg ccagttgttt tgtgatattt tctcgacagc atgctcccct
    11401 cccattgcac aggcgttaaa ctgacgacgt gcactgcaat ctcgtccagt ggcagaccta
    11461 cacgacgtcc gccttgcgcc cctcgtcttt gctcttgacg acggtgtcgt ggggccgctc
    11521 gccgaggctc ctcgtcctgt agccggcttg ccaatcggtg acgaaacgaa cggcgcgccg
    11581 ttcgcgccgc ccacttccac tccacgccct ggaatgccgc cctcccgtat tgagtggggc
    11641 agtcaaatga aggccccttc ggccacccca gccattgaga gacggcgaga cgggcttcaa
    11701 ttcggaccac cgaatgtcat ggctgacgtg acattgatga atatgatcgt tcttactggc
    11761 aagtcttgac gctgcctttg gtcgttgccc tgcaacgcaa gccaagacaa cagggctggg
    11821 cgcccggcac gctgtcgcag tcatagccat tctgcaggca acggctctga acggtccgga
    11881 gaagagactg ggttttccca gcgtccggaa cattcagctc attaaaccca acgaccaggg
    11941 cctgggcggc gacactcagc tcgctggtcg ccgatgtggc gctattggcg cccattgtta
    12001 cgacgccgcc aagggccacc acgcccgcgc agacgaaacc aagtacgcgt catgggattt
    12061 tcattgtgac gaatttacct ccacgcaatt tgaccaagtt tggtcatgat ggcatagggg
    12121 gggggggagg caagcatttc ccaattattt ttgaagcgca ccaaaatgtc gtgagcacgt
    12181 agttcgtgag gtgtcgcgtc cgagcgcaag atgggccgat gggcgtgaca cgcgtgttgc
    12241 cgcgatgctc atcggcgcag gtcatgcatt tgcgaccagt cgtcgggctt agcgctcagc
    12301 gtcgactgac ccagcttggt gacagtagtt gggttcatgc aggggccatg aactcgaaaa
    12361 ctctgggctg aggtcagcgt cagctttaag gcgtaccact gccttccaaa gcacacacca
    12421 agaacagcag catcggctca aaaccgacca gtcactcgct cagtatcgtc ggttcgacgc
    12481 accgctgccg cacctcacct cacctcacct catctcgcca aacagctgcg ctgccgcgtc
    12541 gcgcagcgcg tcgatgccgg cgccggtctc ggatgatgtc gcgatgatct cggggaatgc
    12601 ggcggcgtgg cgggcgatcg cggcgcgcgt tgcggccagg acggcgtcga gggctttcgg
    12661 cgcgatcttg tcgatcttgg tcagcacgat ctgataggag acggccgcct cgtcgagcag
    12721 cgccatgatc tcctcgtcta ctgacttcac gccgtggcga ctgtcgatca gcagatagac
    12781 gcgggcgagc gcctgtcggc cgcgcaggta gtccttgatg aggccggtcc aggccttcac
    12841 cttttccttg ggcgcctcgg cgaagccgta gcccggcagg tcgacgatcg cgagcgccac
    12901 ctcgtcggcg acgaagaagt tgagctcctg cgtgcggccg ggcgtgttgg aggtgcgggc
    12961 gagccccttc tggcgcacga gtgcgttgat gaggctcgac ttgccgacat tcgagcgccc
    13021 ggcgaaggcg acc
//
LOCUS       DAORNH010000109        12827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3687_length_12827_cov_2.804338, whole genome shotgun sequence.
ACCESSION   DAORNH010000109 DAORNH010000000
VERSION     DAORNH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3687_length_12827_cov_2.804338"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PK264_12305"
     CDS             complement(<1..510)
                     /locus_tag="PK264_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRD76700.1"
                     /translation="MASRLTALQRRRQHLKERLIKAALFAAAASSVLVTLGIVGVLLY
                     ESFEFFRSVPILTFLTDTEWTPLFDNPRFGMLPLLVGTLLSTLVALAIAAPLGLTAAL
                     WLSEFAHPRTREILKPILELLSGVPTVVYGFFALLFVTPLLQKTFLPNLPGFNILSAG
                     IVIGIMILPY"
     gene            complement(645..1655)
                     /locus_tag="PK264_12310"
     CDS             complement(645..1655)
                     /locus_tag="PK264_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD76701.1"
                     /translation="MTSIARHTMRTAGLAGVALAALAAPLHAQPKTITLDGSSTVFPI
                     MEAVAEEFQKSTKGANRVTVGISGTGGGFKKFCRGETDMSNGSRPILKEEIEACKKAG
                     IGFIELPLAFDALTVVVHPSNPLTCIKVSELKTMWEPGAKGRITNWKQINPSFPDLAL
                     KLFGAGADSGTFDYFTEAAVGKAKSSRGDYTASEDDNVLVKGVQGDKGGIGYFGFAYY
                     AANPKALKALEIDWGKGQGCVAPSLENVLAGKYNPMSRPLFVYVSSKAVEKPEVQSFV
                     EFVLTKGPALIREVKYLPLPEAIYPTVLERFKARTPGSAFGGVPEVGVPIADLMKRKP
                     TM"
     gene            complement(1790..3067)
                     /locus_tag="PK264_12315"
     CDS             complement(1790..3067)
                     /locus_tag="PK264_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD76702.1"
                     /translation="MTNHHRQVSDLIRGRLSQSREAIAVAALTSAILVWIGGLTWWLA
                     ALGPAIGAIWVVVAAFVTPTTAPSLTRHDSSAADPGRPRPSGPGWLAVVDMLPDPVLT
                     LDRSGRVTHANTAALALFPGAPGRHVAQLHRSPELFAAIDRAWNERGTHTFAIHDVVP
                     HERHLSGIVATLGDGAVGLPEPVQVVILRDRTEAELAAEMRADFVANASHELRTPLAA
                     LKGFIETLQGAAKDDPVARDQFLGIMQEQAARMSRLIDDLLSLSRIEMREHVAPTGRV
                     DLNAAAAEAVALLRPLAESGTITLTLAPVATTAIAAGDHDEIVQVAQNLIQNAIKYGR
                     RGGKVDVSVLREQAHVSLRVSDDGIGIAPEHLPRLTERFYRVSARESRERGGTGLGLA
                     IVKHIVNRHRGELEIDSQPGRGSIFTVRLLAAT"
     gene            3658..5235
                     /locus_tag="PK264_12320"
     CDS             3658..5235
                     /locus_tag="PK264_12320"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRD76703.1"
                     /translation="MTDIAQPQKPRSDTDRVMIFDTTLRDGEQSPGAAMTLDNKLSVA
                     RLLDEMGVDIIEAGFPASSNGDFEAVAEIAKVVKRAVVCGLARSSLGDIDRAGEAVKH
                     AVRPRVHTFISTSPVHRKYKLNMSAEQVIDAVAKSVERARRYTDNVEWSAEDATRTER
                     DVLLKCCEVAIKAGATTINIPDTVGYSVPEEYFALIKMLRETVPGGDTVVWSTHCHND
                     LGMAVANSLAGVRAGARQIECTINGLGERAGNAALEEIVMAMNVRNDVLPYWNEVDTT
                     KFVRASKLVAAVSAFPVQYNKAIVGRNAFSHESGIHQDGMLKNAETYEIMRPEAVGLS
                     KSHLSLGKLSGRAAFRDKLKALGYELGDNAFQDAFRRFKDLADKKKLVFDEDIEALVD
                     QEVASQSDRIKVVALTVIAGTGGPQKATITLEVDGRHETREASGDGPVDATFKAIKAI
                     VPHEVKLPLYQVMAVTEGTDAQAEVMVRLEEAGRTVTGRGADTDTMVASARAYVSALN
                     KLLMKRQRGPSAQSMAS"
     gene            5338..6228
                     /gene="fdhD"
                     /locus_tag="PK264_12325"
     CDS             5338..6228
                     /gene="fdhD"
                     /locus_tag="PK264_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HRD76704.1"
                     /translation="MTDEPRAPEFLIRPDPDDTRLYSVVGGLDHEGRRIESSVVAERP
                     LTLFLNAREIVTMMTIGDHPELLAVGYLLNQGMLRPDDEITGVEHAEDIGTIVVRTHR
                     QTDFEEKLRKKVQTSGCAQGTVFGDLMDQLEAVNLDPARRLRTSWLYTISKKINLAPS
                     LYLKTGAIHGCALCEEDRPLVYMEDVGRHNAVDKIAGYMFRNHVDPASKIFYTTGRLT
                     SEMVIKTVRMGIPILVSRSGFTAWGVELARKSNLTLIGRVRGRRFVCLSGEERLVFDA
                     DPKAVDEEERRHRRKGADDM"
     gene            6336..7241
                     /locus_tag="PK264_12330"
     CDS             6336..7241
                     /locus_tag="PK264_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD76705.1"
                     /translation="MEQFLQQMAAGLANGAIYACVALALVMIFVSTDHINFAQGEMAM
                     FSTYVAWTLIQAGTGFWLALLLSVAASFIVGVLIERAILRPLHNAPVLSIVVVFIGLL
                     AIFHSMAGSIWTHTLKTVASPFPAVKFPGSSIIGPHQIGMIAVTVALLFVLFAFFRFT
                     PLGLAMRAAAQNPASARLVGVSVDWMLALGWGLAAAIGAVAGAMVAPAVNLDPNMMAG
                     ILLYAFAGALVGGISSPGGAVAGGFIVGLLENFVAFIGDRIETWTGYYIIGNGEKLTV
                     ALIIVITVLTLKPSGLFGRVDVKRV"
     gene            7243..8298
                     /locus_tag="PK264_12335"
     CDS             7243..8298
                     /locus_tag="PK264_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD76706.1"
                     /translation="MAEQTASPAVVAAPAARSSFNFAPLVVWPLVAVAVALPFISNAG
                     GGLISNFSFLQLSLVIVYSIAVLGLNLLTGFNGQISLGHGAFFAVGAYVAAILIKHFD
                     IPYWSVLPVAALACFVVGYLFGLPALRLEGHYLALATFALAIAVPQFLKYRHFEAWTF
                     GVQGITLRRVRGPEWTGLNTDQWLYLVVLLIAVLMFWLARNLINSRAGRAMMAIRDHH
                     MAADTMGIDTARTKAITFGISALYTGLAGALHAIIFKFVSPDSFPFPLSISFLVGGVV
                     GGIASLPGAVIGGAFVQVIEKYADALTKTISQMVHLPIELQPWTIYGIVLIILIYVMP
                     SGIAGGIASLWARLRGR"
     gene            8427..9590
                     /locus_tag="PK264_12340"
     CDS             8427..9590
                     /locus_tag="PK264_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76707.1"
                     /translation="MPAIAQAKKYDVGATDTEIKIGNTNPYSGNASSYGNIGRTLDAV
                     FKMVNDQGGIGGRKINFVTYDDGYSPPKTVEMVRKLVEVDKVLLLFQTLGTPPNTAIH
                     KYMNDEKVPHLFVATGASKWGQPKKFPWTMGWQPDYATEGAIYAKHILATKPGAKIAV
                     LMQNDDYGRDYYNGFLAGLTKANEKMIVSRATYEVTDPTVDSQMIQLKNSGADVFFNI
                     TIPKFATQAIKKAHELGWKPVHYLNNVSSSFSTTMRPAGIEACQGIILALYRKDVADA
                     QFADAADVKAFRAFMAKYMTASDYKDDLHNYGYSVAHTLIEVLKKCGNELTRENVMRQ
                     AANLKGFEAPLLLPGIKINTSPTDFYPIQAVRLARVKGEGFELFGDVLASEST"
     gene            complement(9660..10235)
                     /gene="pyrE"
                     /locus_tag="PK264_12345"
     CDS             complement(9660..10235)
                     /gene="pyrE"
                     /locus_tag="PK264_12345"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRD76708.1"
                     /translation="MLPTASRARLIEIITRRSYGTGVEIKLASGRTSNFYFNMKPTML
                     DPEGAYLIGALLSDAITPGEADMIGGLEVGAIPLAAAVAAVSHAKGQPMPAFFIRKQA
                     KGHGTQSLVEGLPRGETLKGRRVIILEDVTTTGGSALKAIEAVRADGAEVVRVITIVD
                     REEGAAEAFAAAGITFSALLQLRDFPPPRDK"
     gene            complement(10467..10772)
                     /locus_tag="PK264_12350"
     CDS             complement(10467..10772)
                     /locus_tag="PK264_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008532553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HRD76709.1"
                     /translation="MSQDDDYVYDDVSGEWLPASELAKKQAAASRVDVRDAVGNVLAD
                     GDQVTLIKDLAVKGAGQTLRRGTVIKSIRLTGDPQEIDCKFDGIKGLVLRAEFVRKR"
     gene            11122..11907
                     /locus_tag="PK264_12355"
     CDS             11122..11907
                     /locus_tag="PK264_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRD76710.1"
                     /translation="MFNPERPPELAVTRWFNAKEPVSLAALKGRVVVLVAFQMLCPGC
                     IEHGLPQAKRLRERFRESEVAVIGLHTVFEHHKVMTVEALAAFMHEYKWPFPVGADEP
                     TGDGIPKTMAAYEMRGTPTMLLFDRQGRLRRHYLGRPDDMQVAAEVMGLVIEEKTASR
                     EQSVAIERALAAGLRGQGEHDHGHDHHHHHHHHDHAHGDACGCGHDHAHDHGHHHDHD
                     HGHHHHAHDGSCCHDHAHEPAPTTKPARKAEAKAETPRPRGSK"
     gene            11904..>12446
                     /locus_tag="PK264_12360"
     CDS             11904..>12446
                     /locus_tag="PK264_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRD76711.1"
                     /translation="MTGGGQRHGHDPRFPGAAYIDPTARITGRVTIGEGASVWPYTVI
                     RAESLEVRIGRRTNLQDHVMVHIGYTTPTLIGDNCSITHRVVLHGCTIGDDCLIGIGA
                     TIMDGAVIGAGSIVAGHAFVREGQVIPPHSIVMGTPGKVVRTIDSRAANRLNAWLYHR
                     NAEHYARGLHRAWDGADFEAA"
     assembly_gap    12447..12474
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12598..>12827
                     /locus_tag="PK264_12365"
     CDS             12598..>12827
                     /locus_tag="PK264_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76712.1"
                     /translation="MLNRLSLAGAAAAFALMLSAPASQISGALLIGSAEAQQPPRCAT
                     APRRCPTGQTARCQREGRCRQGTRVVNACLQFR"
BASE COUNT         2225 a         4140 c         4234 g         2200 t
ORIGIN      
        1 gtagggcagg atcatgatgc cgatgacgat gccggccgag agaatgttga agccgggcag
       61 gttcggcaag aatgtcttct gaaggagcgg cgtcacgaac agcagcgcga aaaatccgta
      121 gaccacggtc ggcacgccgg acaggagctc gaggatcggc ttcaggatct cgcgcgtgcg
      181 cggatgggcg aactcgctga gccagagcgc cgccgtgagg ccgagtgggg cagcgatcgc
      241 gagtgccacc agcgtcgaga gcaaggttcc gacgagaagc ggcagcatgc cgaagcgtgg
      301 attgtcgaag agcggcgtcc actcggtgtc ggtgaggaac gttagtatcg gcacgctcct
      361 gaagaattcg aaagactcgt acaacagcac gccgacgatg ccgagtgtca ccagcacgga
      421 cgaggctgcc gcggcgaaca gcgcggcctt gatcaggcgc tccttcaagt gctggcgccg
      481 ccgctgcaat gccgtgagac gagatgccat gcgtcaatct ccacgtgacc gagcagagat
      541 acggccgggc cgtatccctg ctctcgccgt ccccagaaca acgcgcgggg tgcgatgctg
      601 ttccctagag ggacggatct tacggccgac tacgcagtac agcgtcacat cgtcggcttt
      661 ctcttcatca ggtcggcaat ggggacgccg acctcgggca cacctccgaa ggccgatccg
      721 ggggtgcgcg ccttgaagcg ctcgagcacc gtcggataga tcgcctcggg cagtggcaga
      781 tacttcacct cgcggatgag ggcaggtcct ttggtgagca cgaactcgac gaagctctgc
      841 acctcgggct tctcgacggc cttcgaggag acgtagacga atagcgggcg cgacatcgga
      901 ttgtacttgc cggcgagaac gttttcgagc gagggggcga cgcagccctg acccttgccc
      961 caatcgatct cgagggcctt cagcgccttc gggttggcgg cgtagtaggc aaacccgaag
     1021 tagccgatgc cgcccttgtc gccctgcacg cccttgacga gcacgttgtc gtcctcgctc
     1081 gcggtatagt cgccgcggct cgacttggcc ttgccgacgg cagcctcggt gaaatagtcg
     1141 aaggtgccgc tatccgcccc ggcgccgaag agctttaggg ccaaatccgg gaagctcggg
     1201 ttgatctgct tccagttggt gattcttccc ttggcgcccg gctcccacat ggtcttgagc
     1261 tcggacactt tgatgcaggt tagcgggttc gacgggtgca cgacgacggt cagcgcgtcg
     1321 aatgccaggg gcagctcgat gaagccaatg ccggccttct tgcatgcctc gatctcctct
     1381 ttgaggatcg gacgcgagcc gttcgacatg tcggtctcgc cgcggcagaa cttcttgaag
     1441 ccgccgccag tccccgagat gccgactgtg acgcggttgg cacccttggt cgacttctga
     1501 aactcttcgg caactgcctc catgatcggg aacacggtcg atgagccatc aagcgtgatc
     1561 gtcttcggct gggcatggag cggcgccgca agtgcggcga gcgcgacgcc ggcgaggccg
     1621 gctgtgcgca tggtatggcg tgcgatggac gtcatggtcc tcgtcctgtt ggtgtgccgc
     1681 agcccatctg cggcagcgtc gtcgtgggtc ccttcgacgc tgcccttcta gccggcgcct
     1741 gcaacaggcg tgtgacagga caaaatattg gaaaggatag gtaaaattgc tacgtggccg
     1801 ccagaagccg cactgtgaat atcgagcctc gccccggctg cgagtcgatc tcgagctcgc
     1861 ctcgatgccg gttgacgatg tgcttgacga tggcgaggcc gaggccggtg ccgccccgct
     1921 cgcggctctc acgagcgctc acgcgataga agcgctcggt gagacggggc aggtgctcgg
     1981 gagcgatgcc gatgccgtcg tcgctcacgc ggaggctgac gtgcgcctgc tcccgcagca
     2041 ccgagacgtc aaccttgccg ccgcgccggc catacttgat ggcgttctgg atgaggttct
     2101 gcgcgacctg cacgatctcg tcatgatcgc ccgcggcgat cgcggtcgtg gcgacgggcg
     2161 ccagcgtcaa ggtgattgtg ccactttccg cgagggggcg aagcagggcg acggcctcgg
     2221 cggcggcggc attgagatcg actctccccg tcggcgccac gtgctcgcgc atctcgatgc
     2281 ggctcagcga gaggaggtcg tcgatgaggc gcgacatgcg cgccgcctgt tcctgcatga
     2341 tgccgaggaa ctggtcgcgg gcgaccggat cgtcctttgc ggcaccctgc agcgtctcga
     2401 tgaagccttt gagggccgct agtggcgtgc gcaactcgtg actggcgttg gcgacgaaat
     2461 cggcgcgcat ctcggcggcg agctctgcct ccgtgcggtc acgcaggatg acgacctgga
     2521 ccggttcggg caacccgacc gcgccatcgc cgagcgtggc gacaattccg gagagatgcc
     2581 gctcgtgcgg gaccacatcg tgaatagcga aggtatgcgt gccacgctcg ttccaggccc
     2641 gatcgatcgc cgcgaagagc tcgggcgatc gatgcagctg cgcgacgtgc cggccggggg
     2701 cgcccggaaa caaggctagc gcagcggtgt tcgcgtgcgt gaccctcccc gagcgatcga
     2761 gggtgaggac cgggtcgggg agcatgtcga cgactgcaag ccagccaggt ccgctcggtc
     2821 tcggcctgcc cgggtcggcg gcgctgctgt cgtgacgtgt gagcgaggga gcggtggttg
     2881 gcgtcacgaa cgccgcaacg acgacccaga ttgcgccgat ggccggtccg agggctgcga
     2941 gccaccatgt gaggcccccg atccatacga ggatggccga cgtcagcgcc gcgacggcga
     3001 tcgcctcgcg ggattgtgac agccggcctc tgatcaggtc tgagacttgg cggtgatggt
     3061 tggtcatcca ggtccgtcgg aggtcgcagc tacgcgttga ggctgcctcg ccgtggccgc
     3121 gcgtccggta agagttgacg ccgtccgtct gcctcagggt gatttagttc acgctctgac
     3181 aataggaggt ggcacacgtc ccttccctct cgatggggga agggccgggg attgggttgg
     3241 tgaggaactg caaacgttgt gcacccccga ccccaaacct cccccgcaag gggggaggga
     3301 gccccctact gccgatccaa aaacgaaact accctgccgc ctcgctacca cgtcgacata
     3361 aacgcttgca aatcgccgac tgctctggct ctatgcgcgg gcgagcggca ggaccgcgcg
     3421 aagcaggtat tggcaaaaac gccatcatga ccagggacca cgagcccagc acctctttgg
     3481 ggctgctccg aacggagccg gccggtgcgg gcctgtccct taccctcctc cttaccagcc
     3541 cctgagccat tcgtggtctc ggtaacgaga cgtgcgctca ggggacccga agacttcgag
     3601 ccggaagcgc aagccgcctc ccgatttccc atgccatcct tggaacggac ctgaacgatg
     3661 accgatatcg cccagccgca aaagcctcgc agcgacaccg atcgcgtgat gatctttgac
     3721 acgacgctcc gcgacggcga gcagtcgccc ggcgccgcca tgacgctgga taacaagctg
     3781 tcggtcgcgc gcctcctcga cgagatgggc gtcgacatca tcgaggcggg ctttccggcc
     3841 tcctccaatg gcgacttcga ggcggtcgcg gagatcgcca aggtcgtgaa gcgggcggtt
     3901 gtgtgcgggt tggcgcgctc gagcctcggc gatatcgatc gcgcgggtga agccgtgaag
     3961 catgccgtgc gcccgcgcgt ccataccttc atctcgacga gcccggtgca ccgcaaatac
     4021 aagctcaaca tgagcgccga gcaggtgatc gacgcggtgg cgaagagcgt cgaacgcgcg
     4081 cgccgctaca ccgacaacgt cgagtggagt gccgaggacg cgacgcgcac cgagcgtgat
     4141 gtgctcctca agtgctgcga ggtcgcgatc aaggccggcg cgaccacgat caacatcccc
     4201 gatacggtcg gctactcggt gccggaggag tatttcgcgc tcatcaagat gctgcgcgag
     4261 acggtgcccg gcggcgacac ggtggtgtgg tcgacgcatt gccacaacga cctcggcatg
     4321 gcggtcgcca attcgctcgc cggcgtgcgt gcaggcgcgc ggcagatcga gtgcacgatc
     4381 aacggtcttg gcgagcgggc gggcaatgcc gcgctcgagg agatcgtcat ggcgatgaac
     4441 gtgcgcaacg acgtgctgcc ctactggaac gaggtcgaca cgacgaagtt cgtgcgcgct
     4501 tcgaagctcg ttgccgcggt ctcggccttt cctgtccagt acaacaaggc gatcgtcggc
     4561 cggaacgcct tcagccacga gagcggcatc catcaggacg gcatgctgaa gaatgccgag
     4621 acctatgaga tcatgcggcc cgaggccgtc ggtctctcga aatcgcacct gagcctcggc
     4681 aagctgtcgg ggcgcgcggc cttccgcgac aagctgaagg cgctcggcta cgagctcggc
     4741 gacaacgcct tccaggacgc cttccgccgc ttcaaggacc tcgccgacaa gaagaagctc
     4801 gtgttcgatg aggacatcga ggcgctggtc gaccaggagg tcgcctcgca gtcggaccga
     4861 atcaaggtgg tggcgctcac cgtgatcgcc gggaccggcg gcccgcagaa ggcaacgatc
     4921 acgctcgagg tcgacggccg ccacgagacg cgcgaggcat cgggcgatgg tccggtcgat
     4981 gcaaccttca aggccatcaa ggccatcgtg ccgcatgagg tgaagctgcc gctctaccag
     5041 gtgatggcgg taacggaagg caccgacgcg caggccgagg tgatggtgcg cctcgaggag
     5101 gcgggccgca ccgtgacggg acgcggcgcc gatacggaca cgatggtggc ttcggcgcgg
     5161 gcctacgtga gcgcgctcaa caagcttctc atgaagcgcc agcgcgggcc cagcgcgcag
     5221 tcgatggcgt cctgagtcgt accggcacgg caacgaaacg ctcgaggggc gctgcccctc
     5281 gagcaccccg ccagggactg ttccctggac cccggcccaa ccagtgcatg agcagccatg
     5341 acggatgagc cgcgcgcacc tgagtttctt atccgacccg acccggacga cacgcggctc
     5401 tattccgtcg tgggtgggct cgaccatgaa ggccggcgga tcgagagctc ggtcgtcgcc
     5461 gagcggcccc tcacgctctt cctcaatgcc cgcgagatcg tgacgatgat gaccatcggc
     5521 gatcatcccg agctgctcgc tgtcggatat ctgctcaacc agggcatgct gcggcccgat
     5581 gacgagatca ccggcgtgga gcatgccgag gacatcggca cgatcgtcgt gcgcacacac
     5641 cggcagaccg actttgagga gaagctccga aagaaagtgc agacctcggg ctgtgcccag
     5701 gggaccgtgt tcggcgatct catggatcag ctcgaggccg tgaacctcga tcccgcccgg
     5761 cgtctcagga cctcgtggct ctatacgatc tcgaagaaga tcaacctcgc gccgagcctc
     5821 tatctgaaga ccggcgccat tcacggttgc gcgctctgcg aggaggatcg cccgcttgtc
     5881 tacatggagg acgtcggccg gcacaacgcg gtcgacaaga tcgccggcta catgttccgc
     5941 aatcatgtcg atccggcctc gaagatcttc tatacgaccg ggcgcctcac ctccgagatg
     6001 gtgatcaaga ccgtgcgcat gggcatcccg atcctcgtct cgcggtctgg ctttacggcc
     6061 tggggggtgg aactcgccag gaaatcgaac ctcacgctca tcgggcgggt gcgcgggcgc
     6121 cgcttcgtct gtctctcggg cgaggagcgg ctcgtgttcg atgccgatcc gaaggcggtc
     6181 gacgaggagg agcggcgcca tcgccgcaaa ggtgccgacg acatgtaagg gccgccgtca
     6241 tcccgcagtg ccgcagcgca ggtctggtgc gcggcctccg gccatgtcat cgatagggac
     6301 gatcacggtc cggttgccgg gggagagtgc cgcggatgga gcaattcctg cagcagatgg
     6361 ccgcgggcct cgccaatggc gcgatctatg cctgcgtggc gctcgccctg gtcatgattt
     6421 tcgtctcgac ggatcacatc aatttcgccc agggcgagat ggcgatgttc tcgacctatg
     6481 tcgcctggac actgatccag gcagggacgg ggttctggct ggcactgctg ctctcggtcg
     6541 ccgcctcctt catcgtcggc gtgctgatcg agcgagccat tctgaggccg ctgcacaatg
     6601 ccccggtcct ctcgatcgtc gtcgtattca tcggcctcct ggcgatcttc cattcgatgg
     6661 cgggctcgat ctggacgcac accctgaaga cggtcgcctc gccgtttcca gcggtgaagt
     6721 ttccgggttc cagcatcatc ggcccgcatc agatcggtat gatcgccgtc acagtggcgc
     6781 tgctgttcgt gctgttcgcc ttcttccgtt tcacgccgct cgggcttgcc atgcgggcgg
     6841 cggcgcagaa tccggcctcg gcccgcctgg tcggcgtcag cgtcgactgg atgctggcgc
     6901 tcggctgggg gctcgccgca gcgatcggcg ctgtcgcagg cgccatggtg gcgccggccg
     6961 tcaacctcga ccccaatatg atggcgggca tcctgctcta tgcgttcgcg ggtgccttgg
     7021 tcggcggcat ctcgagcccc gggggggcgg ttgccggcgg cttcatcgtc ggtctcctcg
     7081 agaacttcgt cgccttcatc ggcgaccgca tcgagacctg gaccggctac tacatcatcg
     7141 gcaatggcga gaagctcacc gttgccctca tcatcgtcat cacggtgctg accctgaagc
     7201 cctccggcct cttcggccgc gtcgacgtga agagggtctg agatggcgga gcagacggca
     7261 agccctgcag tcgtggccgc gcctgccgcg cgctcatcct tcaatttcgc gccgctcgtt
     7321 gtctggccgc ttgtcgcggt tgcggtcgcg ctgcctttca tctcgaacgc gggcggcggg
     7381 cttatctcga acttctcgtt cctgcagctg tcgctggtga tcgtctactc gatcgcggtg
     7441 ctcggcttga acctgctcac gggcttcaac ggccagatct cgctcggcca tggcgccttt
     7501 ttcgcggtcg gggcctatgt ggccgcgatc ctcatcaagc acttcgacat tccgtactgg
     7561 agcgtgctgc cggtcgcggc gctcgcctgc tttgtggtcg ggtatctctt cgggctgccc
     7621 gcgctgcggc tcgaggggca ttatctggcg ctcgccacgt ttgcgctcgc gatcgcggtg
     7681 ccgcagttcc tgaaataccg ccatttcgag gcctggactt ttggcgtaca aggcatcacc
     7741 ctccgccgtg tcaggggccc ggaatggacg ggcctcaaca ccgatcagtg gctctatctc
     7801 gtggtcctgc tgatcgccgt cctgatgttc tggcttgcgc gcaacctgat caacagtcgc
     7861 gcggggcgtg ccatgatggc gatacgcgac caccacatgg cggccgacac catgggcatc
     7921 gataccgcgc gcacgaaggc aatcaccttc ggcatcagcg cgctctacac gggccttgcc
     7981 ggcgcgctgc acgccatcat cttcaagttc gtctcgccgg acagcttccc gtttccgctg
     8041 tcgatctcgt tcctggtcgg cggcgtggtc ggtggcatcg cctcgctgcc cggcgccgtg
     8101 atcgggggcg ccttcgtgca ggtgatcgag aagtatgccg acgcgctgac caagaccatc
     8161 tcgcagatgg tgcacctgcc gatcgagctg cagccttgga cgatctatgg catcgtgctg
     8221 atcatcctca tctatgtcat gccgtccggg atcgccggcg gcattgcctc gctttgggca
     8281 cgattgcggg gacgataacg tctcgaacgt gtgtgccatc caacgacgaa cagaagacgg
     8341 tcactcggga ggaaccaatg accaagacca cacgccgcag cgtcatcatc ggcggctcga
     8401 cgctcgccgc gggccaggtg atcggcatgc cggccatcgc ccaggcgaag aagtacgacg
     8461 ttggcgccac cgataccgag atcaagatcg gcaacaccaa cccctactcg ggcaacgcct
     8521 cgagctacgg caacatcggc cgcacgctcg atgccgtctt caagatggtc aacgatcagg
     8581 gcggcatcgg cggccgcaag atcaacttcg tcacctacga cgacggctac agcccgccga
     8641 agacggtcga gatggtgagg aagctcgtcg aagtggacaa ggtgctcctc ctcttccaga
     8701 cgctcggcac gccgcccaac acggcgatcc acaagtacat gaacgacgag aaggtgccgc
     8761 atctcttcgt cgcgaccggg gcctcgaagt ggggccagcc gaagaagttc ccctggacga
     8821 tgggctggca gcccgactat gcgaccgagg gcgcgatcta tgcgaagcac atcctcgcga
     8881 ccaagcccgg cgccaagatc gccgtgctga tgcagaacga cgactatggc cgcgactact
     8941 acaacggctt cctcgccggg ctcaccaagg cgaacgagaa gatgatcgtc tcgcgggcca
     9001 cctacgaggt gaccgacccg accgtcgaca gccagatgat ccagctgaag aactcgggcg
     9061 cggacgtgtt cttcaacatc accatcccga agttcgcgac gcaggccatc aagaaagcgc
     9121 acgagctcgg ttggaagccg gtgcactatc tcaacaacgt ctcgtcgtcg ttctcgacca
     9181 cgatgcgccc cgcgggcatc gaggcctgcc agggcatcat cctcgcgctc taccgcaagg
     9241 acgtcgccga cgcacagttc gccgatgccg cggacgtgaa ggccttccgc gccttcatgg
     9301 cgaagtacat gacggcctcc gactacaagg acgacctgca caattacggc tactcggtcg
     9361 cccacacgct gatcgaggtg ctgaagaagt gcggcaacga gttgacgcgc gagaacgtca
     9421 tgcgccaggc cgcgaacctc aagggcttcg aggcgccact gctgctgccc gggatcaaga
     9481 tcaacacgag cccgacggac ttctatccga tccaggccgt gcggctcgcg cgcgtgaagg
     9541 gcgaaggctt cgagctcttc ggcgacgtgc tcgcgagcga gagcacctga ggggcgggtg
     9601 tcgaacgcta tcgatcggtg tcagacccat gggtctgaca ccgatcgcgt agggcgtcgt
     9661 cacttgtcac gcggcggcgg gaagtcgcgg agctggagga gtgccgagaa tgtgatgccg
     9721 gccgcggcga acgcctcggc ggcaccctcc tcgcggtcga cgatggtgat gacgcgcacg
     9781 acctcggcgc cgtcagcgcg gacggcctcg atcgccttca gcgccgagcc gccggtcgtc
     9841 gttacgtcct cgagtatgat gacgcggcgg cctttgagcg tctcgccgcg cgggagccct
     9901 tcgacgaggc tttgtgtgcc gtggcccttg gcctgcttcc ggatgaagaa ggccggcatt
     9961 ggctgtccct tggcatggct gacggcagca acggcggcgg cgagcgggat ggcgcctact
    10021 tcgagcccgc cgatcatgtc ggcttcgccc ggcgtgattg catcagagag cagcgcgccg
    10081 atcaggtagg cgccctccgg atcgagcatc gtcggcttca tgttgaagta gaagttcgag
    10141 gtgcggcccg aggcgagctt gatctcgacg ccggtgccat aggagcgccg tgtgatgatc
    10201 tcgatcaggc gggcgcggga tgcggtcggg agcatggggc ctcgctgctg gattgtctcg
    10261 gcggcgagtg agcctgcggc gggcgccatg tcaaggcttg gccttgcccc tgcacccgaa
    10321 caaggagcgg atctgaaggc cgtcatcctg tagcgatgct ccgcatcgac ggcctttggc
    10381 tgggttcggg gcagcgcccc gaaatgccaa gcgcgatcct cccgcgtcag tgagtttgct
    10441 cagcacgccg cacggcatgg cccccgctac cgcttgcgca cgaactcggc acgcagcacg
    10501 agccccttga tgccgtcgaa tttgcaatcg atctcttgcg ggtcgcccgt caggcggatc
    10561 gacttgatga cggttccgcg tcgcaacgtc tggcccgcac ccttgaccgc gaggtccttg
    10621 atcagcgtca cctgatcgcc gtctgcaagc acattgccga cggcatcgcg cacgtcgacc
    10681 cggctcgcgg ccgcctgctt cttggcaagc tcggaggctg gcagccactc gccgctgacg
    10741 tcgtcataga cgtagtcgtc gtcctggctc atgtcctagc tcctgatcga aggcgatcct
    10801 accgagtttg ctgccaggat tgagagccaa atcgatggtg ttcggtccgc aacccgggat
    10861 acgggtccct tagaccggcg tgagcgagcc tgtccagggg ctgcgggtga gaccccgagg
    10921 gggcggcacc tgcaattccc actcgcaacg gtggggagca catggccgac gagcaagctg
    10981 atcagctgga ccgggcgtat ggcgcggcgc gaagtttcgg gccactcact tgtgacgcct
    11041 gagtgagagg ccaacgcttg agcaggccct ttgcgtcggc tactcatggc gcaccgcaac
    11101 agtcgccggc aaggagccgc catgttcaat cccgagcgcc cacccgagct tgccgtcacg
    11161 cgctggttca acgcgaagga gccggtctcg ctcgccgccc tcaagggccg ggtcgtggtg
    11221 ctggtcgcct tccagatgct ctgcccgggg tgcatcgagc acgggctgcc gcaggcgaag
    11281 cggctccgcg agcgcttccg cgagagcgag gtcgcggtga tcggcctgca cacggtattc
    11341 gagcatcaca aggtaatgac ggtcgaggcg ctggccgcct tcatgcacga gtacaagtgg
    11401 ccgttcccgg tcggcgccga cgagccgacg ggcgatggca tcccgaagac gatggcggcc
    11461 tacgagatgc gcggcacgcc gacaatgctg ctgttcgacc ggcagggccg cctgcggcgc
    11521 cactatctcg ggcgcccgga cgacatgcag gtggcggccg aggtgatggg cctcgtcatc
    11581 gaggagaaga cggcgagccg cgagcagtcc gtcgccatcg agcgggcgct ggcagcgggc
    11641 ctccgcggcc agggcgagca tgatcacgga cacgatcatc atcaccatca ccatcaccac
    11701 gatcatgcgc acggcgacgc ctgcggctgc ggacatgacc acgcgcacga tcatggccac
    11761 catcacgacc atgatcacgg ccatcaccat catgcccatg atggctcgtg ctgccacgac
    11821 cacgcgcacg agcctgcgcc aacgaccaaa ccagcccgca aggcggaagc gaaggccgag
    11881 acgccgaggc cgcggggcag caaatgacgg gcggcggcca gcgccacggc cacgacccgc
    11941 gctttcccgg cgccgcctac atcgatccga cggcgcgcat cacgggtcgc gtcaccatcg
    12001 gcgagggcgc gagtgtgtgg ccctacactg tgatccgcgc cgagagcctg gaggtgagga
    12061 tcggccggcg gaccaacctg caggaccatg tgatggtcca catcggctac acgactccga
    12121 cactgatcgg cgacaactgc tcgatcaccc atcgcgtggt gctgcacggc tgcacgatcg
    12181 gcgacgattg cctgatcggt atcggtgcca cgatcatgga cggcgcggtg atcggcgcag
    12241 gctcgatcgt tgccgggcat gcgttcgtgc gcgaggggca ggtgatcccg ccgcactcga
    12301 tcgtcatggg cacgcccggc aaggtcgtgc gaacgatcga cagccgcgcg gcgaaccggc
    12361 tcaatgcctg gctctatcat cgcaacgccg agcactacgc gcgggggctg catcgcgcct
    12421 gggacggggc tgatttcgag gcggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcagg
    12481 tgtctgaggc cggcgcctga aacacgatcg gaacagacgg caggcgatcg gcgctgtatg
    12541 gtcaggcttg ccttgggttc ttgccattca ttggaacgac gcgacaagga gatggtcatg
    12601 ctcaatcgat tgtcattggc cggagcggcc gcagcatttg ccctcatgct ttcggcgcca
    12661 gcgagccaga tcagcggcgc actgctgatc gggtccgcgg aggcacagca gccgccccgg
    12721 tgcgcgacgg cgccgcgccg ctgcccgaca ggccagactg cgcgctgcca gcgtgaaggc
    12781 cgctgccgcc agggtacccg cgtcgtcaac gcctgcctcc agttccg
//
LOCUS       DAORNH010000110        12793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3701_length_12793_cov_2.718873, whole genome shotgun sequence.
ACCESSION   DAORNH010000110 DAORNH010000000
VERSION     DAORNH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3701_length_12793_cov_2.718873"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK264_12370"
     CDS             complement(<1..317)
                     /locus_tag="PK264_12370"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HRD76713.1"
                     /translation="MVIATLAILAGSLPAEAEGVLVTAIGLRGRSDGFAYAWSANHSS
                     ARSATEAALAECRKQAERVRVPTSACTVTLNFTDQCVSVVFDPKSGAAGIGWATGPDR
                     VSA"
     gene            695..1474
                     /locus_tag="PK264_12375"
     CDS             695..1474
                     /locus_tag="PK264_12375"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HRD76714.1"
                     /translation="MLTRQLVAAGAGALILMATPQPVAAHPHVWVKVDTTVVYERGTI
                     VALKQRWVFDEVYSSFAIDGLDKNKDGVFSREELQELAKANMDGVGQFGYFTSAKLDN
                     APLKFGAPTEFYLEHIIDPNPRPKGAGPATKTEPKSSSMLGGIWNWMVGDDDARKNEP
                     AKVLALTFTLPLSQPVLAEAEGFSFLTRDPTSYIAFGLAAAEPVRLSAGAPKECKARI
                     DAADLDPETKRLEEAFSGAGDGIRWSQLLGSSITIVCDGKS"
     gene            1471..2547
                     /locus_tag="PK264_12380"
     CDS             1471..2547
                     /locus_tag="PK264_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HRD76715.1"
                     /translation="MKIVTLGRGAAAFGAAGLLALVLSSEVAAQLASTPPVAKPGGIW
                     ATILSAQQDVQTALGRALERIKTTEPLKATAFLALLSFVYGVLHAVGPGHGKAVISSY
                     VLANERTMRRGIVLSFLSAFAQAIVAILLVVVLRLVLRLTQLETRLIEPWLDVISWGL
                     VAAVGAWLLWREVRRMLRVRAARGVGEHAHDHHAHSHHAHSHHDHAHHHDHTHPAAHG
                     RIPHGAPGHVHDEHCGHAHMPGPEALEGEWSWRRAFPIILAVGLRPCTGAIVVLVAAS
                     GLGLFWAGVISTFVMSLGTAITVSALAALAVGSRELAIRLFGGDGRRIGALRSIATIA
                     GAVTLLAFGTLFLLASLTTGSRAF"
     gene            complement(2813..3556)
                     /locus_tag="PK264_12385"
     CDS             complement(2813..3556)
                     /locus_tag="PK264_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76716.1"
                     /translation="MARAPRTALVTGSGKNIGRASALVLARDGYNVVLNGSRDKLACE
                     TVAREVRQLGVDAYVAMGDVGDRIAIGRIIDEALTTFGTIDVLVNNAAVREDGAFLAM
                     SEQAWRRAMAINFDSAYWLSRAFLPGMIAKGFGRIINFAGMNAIHGYNGRAHVSASKH
                     AAWGLTKALAKEFGGRGVTANIISPGPIMGEAQEAHQAAHIEGMRSRVPVGRLGTPDE
                     VAAVVALLASERGAFINGQLIQVNGGAET"
     gene            3879..>4998
                     /locus_tag="PK264_12390"
     CDS             3879..>4998
                     /locus_tag="PK264_12390"
                     /inference="COORDINATES: protein motif:HMM:NF023990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM143 family protein"
                     /protein_id="HRD76717.1"
                     /translation="MTTATARLEPASAGASPAGTAESTAPEMSEVIRKAPSDGDMREK
                     FIPVTRHALIERLTRPHVWAAGEAAHARRFLGHLSYWRGQSYNKVLLDLEQTYEPFSP
                     DSDLLVTRKFTDAERLEMQHRVVDQMAKLLRKANFVQVDTTKIKEILTNETHYGLDLE
                     IDLADFEELLVFYRGATSRKASKRTLRKLFIYKEEYDVPIFQRLFILFKLKSFDARVG
                     EVMREANLERKDAEKRVKKARSSLPGEVKTDFVYMKLFKSLPQSDLEMVFPNGRVKFR
                     MFDKLKLGVTAGGGLGMGVFGTAGKIAVATNPIALAGAVAGLGGIAARQVFAFLNQRT
                     KYMVTLAQNLYFHSMADNRGVMTLLCDRASEEDVKEEAL"
     assembly_gap    4999..5027
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5028..5354
                     /locus_tag="PK264_12395"
     CDS             <5028..5354
                     /locus_tag="PK264_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76718.1"
                     /translation="HRSELHHVDEAIEQYLVNSFGIDANYDIYDALDRLMRDGIVSED
                     AEGYLVALPPSGAAKQIDILWDAYLDELPDVAGAEGEEMDTMIDPRSATLEAAPQSED
                     QWLTTS"
     gene            5336..6214
                     /locus_tag="PK264_12400"
     CDS             5336..6214
                     /locus_tag="PK264_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRD76719.1"
                     /translation="MADHKLSFHTSDRDAAWKIGGMLGEALDPAPGAVAVFETQGPAG
                     WVVNCYFEAEPDLVAIRGLLAVDPDLATLEPRLEAIPDYNWVAISQAALPPVEAGRFI
                     VRGSHDRARTRHRRNAIEIDAGEAFGTAHHATTYGCVLAIDRHARRRPPRRILDLGCG
                     SGVLAIAAAKAWPAALIEASDIDATSVEVARANAAINGVAPRIRFRTGAGLPATAGSP
                     ARQYDLLIANILAGPLKRLSAPIALAVRRGGTVILSGLLVREAATVAASYVSKGFELC
                     SRRDHTGWTVLELVRR"
     gene            6345..7352
                     /gene="hemH"
                     /locus_tag="PK264_12405"
     CDS             6345..7352
                     /gene="hemH"
                     /locus_tag="PK264_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HRD76720.1"
                     /translation="MGHPDVRLGKIGVLLLNLGTPDATDYWSMRRYLKEFLSDRRVID
                     LPRWKWWPILNLIILTVRPSRKGRDYAAIWNKERNEGPLKTITRAQAEKLATRLAGSA
                     KIVVDWAMRYANPTTASRLEALRAEGCDRILIVPLYPHYAGATTGTAADQAFRALMSM
                     PWQPAIRLAPAWGDDPVYIRALAQSIRHHLGTLDFEPERLVVTYHGMPQRYLLQGDPY
                     HCQCLKSSRLLAAELGWPEGRMLTSFQSRFGTEEWLRPYTDETITGLAKSGVKRIAVV
                     APGFTADCLETLEELDVENRGFFTSNGGTHFSYIPCLNDSDPGLDVIEAVVRRELAGW
                     A"
     gene            complement(7386..8663)
                     /locus_tag="PK264_12410"
     CDS             complement(7386..8663)
                     /locus_tag="PK264_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HRD76721.1"
                     /translation="MAAPRPSKTHIGNHVLKPETLMLGYGFDPMLSEGAVKPPVFLTS
                     TFVFGTAEEGRDFFDYVSGRKTPPAGAGAGLVYSRFNHPNSEIVEDRLAIYEGAEAAI
                     LFSSGMSAIATTLMAYARPGDVIVHSQPLYGGTETLLTRTFAEFGVRSVPFSDGVSET
                     TIRKALDRAKQAGRIAVVLVETPSNPTNTLVDIALVRRLTDDLGASQAHRPIIACDNT
                     LLGPVFQHPLDFGADLSIYSLTKYVGGHSDLIAGAALGKKSAIGPVKALRSAIGTQLD
                     PHSCWMLGRSLETLSIRMEKANANAKIAAEFLRDHPRVGKVHYLPFLGAGAERATYER
                     QCTGAGSTFSFDIKGGQAEAFAFLNALQVFKLAVSLGGTESLASHPATMTHSGIPVDV
                     RQRIGVLETTIRLSVGIEHPDDLVADLAQALAR"
     gene            8803..9249
                     /locus_tag="PK264_12415"
     CDS             8803..9249
                     /locus_tag="PK264_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRD76722.1"
                     /translation="MILDDVDRRILHVLAGNGRITNQELADKVGLSPSPCLRRLRRLE
                     DIGVIKGYTVLVDPKAHGWFMSAIATIRLTRQNEDDIEMFERAVQAWPEVSECYLVTG
                     SRDYILKIMASDLPDYERFIKGRIARLKCVASIETSFIMNTIKGRG"
     gene            9324..10409
                     /locus_tag="PK264_12420"
     CDS             9324..10409
                     /locus_tag="PK264_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD76723.1"
                     /translation="MNLAHTLQVAALQYPDRPAVSEEGRTLDYGAMEVLVGRIAGGLR
                     ARHGLKPGDRVALWMDNTLEVLPLLYGIWRAGLTAVPVNAKLHAKELAWIVADSGAQL
                     LFVTDALGAKLSELPSGASLPRVIAATAKEIAALATADPLTRGPTDPSDEAWLFYTSG
                     TTGRPKGAVLTHRNLLFAVHAYYADIDFLGPSDTILHAAPLSHGSGLYGLAFVARGGH
                     NVIIPGSFEPDRIYRAIAEHSDVSFFAAPTMVSRLINHPLAGSTDTQNLKTISYGGAP
                     MYVADLERALELFGPKLFQLYGQGESPMTITGLGKAAHAARHRATASGSARQVSRAPA
                     SLSAWWATTGPSCRRARSARSSHEATA"
     gene            10337..10846
                     /locus_tag="PK264_12425"
     CDS             10337..10846
                     /locus_tag="PK264_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD76724.1"
                     /translation="MVGDDGAELPPGEIGEIVTRSDCVMKGYWNNPEANAKAIRDGWL
                     WTGDMGAADSDGFLTIKDRSKDMIISGGSNIYPREIEEVLARHPAVLECAVVGRIHPE
                     WGEEVVAFVVARPGTRITAPELDRLCLDNIARFKRPKDYRFVDALPKNNYGKILKTEL
                     REVFPEKKS"
     gene            10916..11368
                     /locus_tag="PK264_12430"
     CDS             10916..11368
                     /locus_tag="PK264_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76725.1"
                     /translation="MTNTSIGNLAAGFIAAALSVLLIHQGMQWVLTQTGLVKLEPWSF
                     KPVGPFSVPTLVNSIFWGGLWGLLFALVWDKVPGSAMWIKGLIYGFVILLISNWTLLP
                     LIKGKIFGQANQALFAGFVPARMLAGLLILGAFGAGTGAIYSLLRSRD"
     gene            11545..11737
                     /locus_tag="PK264_12435"
                     /pseudo
     CDS             11545..11737
                     /locus_tag="PK264_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711103.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     assembly_gap    11738..11747
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11748..12232
                     /locus_tag="PK264_12440"
     CDS             <11748..12232
                     /locus_tag="PK264_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76726.1"
                     /translation="NAGIAGPTAKAWEYPLAELRRTMAVNLDGPFNCCHAVVPGMVSR
                     NYGRIVNIASIAGKEGNPNASAYSASKAALIGFTKSLAKELATHDIAVNAIAPAVART
                     AILGQVTQAHIDYMISKIPRGRTVEPSEIAALIAWAASADCSFTTGSVLDISGGRATY
                     "
     gene            complement(12251..>12793)
                     /gene="recR"
                     /locus_tag="PK264_12445"
     CDS             complement(12251..>12793)
                     /gene="recR"
                     /locus_tag="PK264_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRD76727.1"
                     /translation="GLGPRSARKAALTLIKRRDDLLRPLAAALASAADRLTICAECGN
                     VDTSAPCTVCADPRRDQSLIVVVEEVGDLWALERAAVLNARYHVLGGHLSPLDGIGPE
                     RLNIAKLIERARPPVVREVLLALNQTVEGQSTSHFIAEQLSGNGVIVSRLAQGVPIGG
                     ELDYLDEGTLAAAIKARRQG"
BASE COUNT         2272 a         4278 c         4033 g         2171 t
ORIGIN      
        1 tcggcgctga cccgatcggg gccggtggcc cagccgatgc cggcggcgcc gctcttcgga
       61 tcgaagacga cggatacgca ctgatccgtg aagttcaacg tgaccgtgca ggcagaggtc
      121 ggtacgcgca cccgctccgc ctgcttgcgg cactcggcaa gtgccgcctc cgttgctgag
      181 cgcgccgagc tatgattggc ggaccaggca taggcaaagc cgtccgaacg gccacgtaga
      241 ccgatcgcgg tcacgaggac gccctcggct tcggcgggca gcgatccggc cagaatggcg
      301 agtgtcgcga tcaccacggc tgttgcggct cgcgacctcg tgccacgagc gcgggaggcc
      361 cgcaggtccc cctgtacgtc tccccgcaga tccccatgtg cacgcatgtc cgatgtctcc
      421 atccgtcacc gatcaggacg cacgcccgcg ttttacacgt catgccgtcg caagaccagc
      481 cgagcgggcc gaagcttacc agattgtcga gcctcggact gaggacatgc tacagcccga
      541 gcggaaaaag gcgatcgccc gcatcaaccg aaaattcgga actgacacat ggcacttcgc
      601 catcatcttg tgataatcgc ttgatctcaa acatcaagcc ggtcgcggtg gtcgccaagc
      661 cgggtgaccg gccaggatcg caggagcgtt ctcgatgttg acacgccaac tcgtcgccgc
      721 aggcgctggc gccttgatcc tgatggcgac gccgcaacct gtcgccgcac acccccatgt
      781 ctgggtaaag gtcgacacga ccgtggtcta cgagcgcggc acgatcgtcg cgttgaagca
      841 gcgatgggtg ttcgacgagg tctattcctc gttcgccatc gacggcctcg acaagaacaa
      901 ggacggtgtc ttttcccgcg aggagctgca ggagctcgcc aaggccaaca tggatggcgt
      961 cggccagttc ggctatttca cctcggcgaa gctcgacaac gcccccctca agttcggcgc
     1021 accaaccgag ttctatctcg agcacatcat cgatcccaat ccgcgaccga agggtgccgg
     1081 ccccgcgacg aagaccgagc cgaagtcctc ctcgatgctc ggcggcatct ggaactggat
     1141 ggtcggcgat gacgacgcca ggaagaacga gccggccaag gtgctggcgc tcaccttcac
     1201 cctgccgttg agccaaccgg tgctggccga ggccgaggga ttctcgttct tgacgcgcga
     1261 cccgacgtcg tacatcgcct tcggtcttgc cgccgccgag cccgtgaggc tcagcgccgg
     1321 cgcgccgaag gagtgcaagg cccgcatcga cgccgccgac ttggacccgg agaccaagcg
     1381 cctcgaggag gcgttctcgg gcgccggcga cggcatccgc tggagccagt tgctcggctc
     1441 gagcatcacc atcgtgtgcg acggcaaatc atgaagatcg tcacgcttgg tcgaggcgcg
     1501 gccgcatttg gcgccgcggg cctccttgcg ctcgtactgt cgagcgaggt ggctgcgcag
     1561 ctggcatcga ctccgcctgt cgccaagcct ggcgggatct gggcaaccat cctctcggcg
     1621 cagcaggacg tccagaccgc gctcgggcgc gcactcgagc gcatcaagac caccgagccg
     1681 ttgaaggcga cggctttcct cgcactcttg agcttcgtct acggggtgct ccatgccgta
     1741 gggcccggcc acggcaaggc ggtcatctcg tcctacgtgc tcgccaacga acgcacgatg
     1801 cgccgcggca tcgtgctctc cttcctgtct gcgttcgcgc aggcgatcgt cgcaatcctg
     1861 ctcgtcgtcg tgctgcggct cgtcctgcgc ttgacccaac tcgagacccg gctcatcgag
     1921 ccctggctcg acgtcatcag ctggggtctc gttgccgccg tcggcgcgtg gctcctctgg
     1981 cgtgaggtgc ggcggatgct acgggtgcgc gcggcccgcg gagttggcga gcatgctcac
     2041 gaccaccatg ctcacagcca ccatgctcac agccaccacg atcacgccca tcatcacgac
     2101 cacacccacc cggcggcgca cgggcggata ccccatgggg cgcccggaca cgtgcatgat
     2161 gagcattgcg gccacgccca catgcccggc cccgaggcgc tcgagggcga atggagctgg
     2221 cggcgcgcct tcccgatcat actcgcggtt ggcctcagac cctgcaccgg tgcgatcgtg
     2281 gtgttggttg cagcttcggg cctcgggttg ttctgggccg gcgttatctc gacatttgtg
     2341 atgtcgctcg gtaccgcgat cacggtgtca gcgcttgcgg ccttggccgt cggctcgcgg
     2401 gagctggcca ttcgcctttt cggtggcgat gggcgccgca tcggcgcatt gcggtcgatc
     2461 gcgaccatcg ccggtgccgt caccttgttg gcgtttggaa ccctattcct gctggcatca
     2521 ctcacgacgg gctcaagggc cttctgagca gcaccggacg tcgcaaacca aaatgccccc
     2581 gctttcgggg ccgctcccga gttcgcgcct gatcaacgcg tgggagctac cctccgaatg
     2641 cggaggcctg acgcatccat gagaccagcg acttacacct gctggaatac tcgaacttta
     2701 cgcggacaca tcgtgcagtc gtacatacca caatcgctgt gtgcggagca tagattcgga
     2761 cagcgcacag gcgagcctgt ggacaacaag tgccgcgttg ccgcaggtca gctcaggtct
     2821 ccgcgccacc gttcacctgg atgagctggc cgttgatgaa ggctccacgc tccgaggcga
     2881 gcagcgccac gaccgccgca acctcgtccg gcgtgccgag ccggcctacc ggcacgcggc
     2941 tccgcatgcc ctcgatgtgc gcggcctgat gcgcttcctg cgcctcgccc atgatcgggc
     3001 cgggcgagat gatgttggcg gtcacgccgc gcccgccgaa ctccttcgcc aatgccttcg
     3061 tgagccccca ggccgcatgc ttcgaggccg agacgtgcgc gcggccgtta tagccgtgta
     3121 tggcgttcat gccggcaaaa ttgatgatgc ggccgaagcc cttggcgatc atgcctggca
     3181 ggaacgcgcg cgaaagccaa taggcggaat cgaagttgat cgccatcgcg cgccgccacg
     3241 cctgctcgct catggccagg aacgcgccgt cctcgcgcac ggcggcgttg ttcacgagaa
     3301 cgtcgatcgt gccgaacgtc gtcagcgcct cgtcgatgat gcggccgatg gcgatcctgt
     3361 cgccgacgtc gcccatcgcg acataggcat cgacgcctag ctggcgcact tcccgggcaa
     3421 ccgtctcgca ggcgagcttg tcgcgcgatc cgttcagcac cacgttgtag ccatcacgcg
     3481 ccagcacaag ggctgaggct cggccgatgt ttttgcctga gccggtgacg agtgcagtcc
     3541 gcggggctct tgccaacgtc atactccgga cagaatgagg cggggtgcat gccatttgaa
     3601 gtttagagcg tcaagccgcc acacggaagg tacacctctg ctgcggtgag cggttgcagc
     3661 ccgattttca atgggatcgc atgagcgctt tagccaggga tgccgaggtc tcgggctttt
     3721 gttcgacggt gtcgtcattt agtatctgca tccgacggag cctggagcca agcggccgag
     3781 gcacaaggtc tcgcgctcac gggccgctcc gtcaccgccg gcacaaggcc tgaacaagcc
     3841 gcgacggcgc caaagcgcat gcacaggggg agggcgcaat gacgactgca accgccaggc
     3901 tcgagcctgc atcagcgggc gcgtcacccg ccggcacagc ggagagcacc gcccccgaga
     3961 tgtccgaggt gattcgcaag gcgccgagcg acggcgacat gcgcgagaag ttcattcccg
     4021 tcacgcggca cgcgctgatc gagcgcctga cccggccgca cgtgtgggcc gcgggagagg
     4081 ccgcgcacgc gcgccggttt ctcggccatc tctcgtattg gcgcggccag agctacaaca
     4141 aggtgctcct cgaccttgag cagacctacg agcccttcag cccggacagt gatcttctcg
     4201 tcacgcgcaa gttcaccgat gccgagcggc tcgagatgca acaccgcgtc gtcgatcaga
     4261 tggcgaagct gctgcgcaaa gcgaacttcg tccaggtcga tacgacgaag atcaaggaga
     4321 tcctcaccaa cgagacgcac tatggcctcg acctcgagat tgatctcgcc gacttcgagg
     4381 aactgctcgt cttctacagg ggggccacat cacgcaaggc gtccaagcgc acactgcgca
     4441 agctcttcat ctacaaggag gagtacgatg tcccgatctt ccagcgcctg ttcatcctgt
     4501 tcaagctgaa gtcgttcgac gcccgcgtcg gcgaggtgat gcgcgaggcg aacctcgagc
     4561 gcaaggacgc cgagaaacgc gtcaagaagg cgaggagcag cctgccgggc gaagtgaaga
     4621 ccgatttcgt ctacatgaag ctcttcaaga gcctgccgca gtccgatctc gagatggtgt
     4681 tcccgaacgg gcgggtcaag ttccgcatgt ttgacaagct caagctcggt gtcactgccg
     4741 gtggcggtct cggcatgggc gtattcggca cggccggcaa gattgcagtc gcaaccaacc
     4801 cgatcgcact tgcgggcgcc gtcgccggtc tcggcggcat cgcggcgcgt caggttttcg
     4861 cgttcctcaa tcagcgcacc aagtacatgg tgacgctcgc gcagaacctc tatttccact
     4921 ccatggccga caaccgaggc gtgatgacgc tgctctgcga ccgcgcatcg gaggaggacg
     4981 taaaggagga ggcgctccnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac aggagcgagc
     5041 tgcatcacgt cgacgaggcg atcgagcagt acctcgtcaa cagcttcggc atcgacgcca
     5101 actacgatat ctacgacgcg ctcgatcggc tgatgcgtga tggcattgtc agcgaggacg
     5161 cggaggggta cctggtggca ctccccccga gcggggcagc caagcagatc gacatcctct
     5221 gggacgccta cctcgacgag ctccccgacg tagcgggcgc cgaaggcgag gagatggaca
     5281 cgatgattga tccgcgctcg gctaccctgg aggctgcccc acaaagcgag gatcagtggc
     5341 tgaccacaag ctgagcttcc acacgagcga tcgagatgca gcctggaaga tcgggggcat
     5401 gctcggggag gcgcttgatc ccgcccccgg cgcagtcgcc gttttcgaga cgcagggtcc
     5461 cgcaggttgg gtcgtcaatt gctacttcga ggcggaaccc gatctcgtcg caatccgcgg
     5521 cctgctggca gtggatccgg atctcgccac tctcgagccg aggctcgagg cgatcccgga
     5581 ctacaattgg gtcgccataa gtcaggcggc gctgcccccg gtcgaggcgg gccgcttcat
     5641 cgtccgcggc agccatgatc gcgcacgcac tcggcatcgg cggaatgcga tcgagatcga
     5701 tgccggcgaa gcctttggca ctgcgcacca tgcgacgacg tacggctgcg tgctcgcaat
     5761 cgacaggcac gcccggcgga ggccgccccg tcgcattctc gacctcggct gcggctcggg
     5821 cgtgctcgcc atcgctgccg ccaaggcctg gccggccgcg ctgatcgagg cgagcgacat
     5881 agatgcgaca tcagtcgagg tcgcccgagc caacgccgca atcaacggtg tcgccccgcg
     5941 catccgcttc aggacgggcg caggcctacc cgccactgcg ggatcaccgg cccgtcagta
     6001 cgatctcctc atcgccaata tcctggccgg accgctgaag aggctctcgg caccgatcgc
     6061 cctcgctgtg cgccgaggtg gaacggtcat cctctcgggc ctgcttgtcc gcgaggccgc
     6121 gacagtggcc gcctcctacg tctcgaaggg gttcgagctc tgctcgcgtc gagaccatac
     6181 cggctggacc gtgctcgagc tggtccggcg ttgagcatgg cggcaaacga tccagacacc
     6241 tcgaatttgc gttagagtct ctcgacccga gttcaggacg agccatgccc cgcgatatga
     6301 ttgatgctgc ccctcctgcc gcgcccgacc ggagcgcctg gccaatgggc catcccgacg
     6361 tcaggctcgg caagatcggc gtgctgctcc tgaacctcgg cacgcccgat gcgacggact
     6421 actggtccat gcggcgctac ctgaaggagt tcctctccga ccgccgcgtc atcgacttgc
     6481 cgcggtggaa gtggtggccg atcctgaacc tcatcatcct gaccgtccgg ccgagccgca
     6541 agggccgcga ctacgccgcg atctggaaca aggagcgcaa cgaaggaccg ctgaagacga
     6601 tcacgcgcgc gcaggccgag aagctcgcga cgcgcctggc aggctccgcg aagatcgtcg
     6661 tcgactgggc catgcgctac gcgaacccga cgacggcgag ccggctcgag gcgcttcggg
     6721 ccgagggttg cgaccgcatc ctcatcgtgc cgctctatcc gcactacgcg ggcgccacca
     6781 cgggaacagc cgcggaccag gccttccgcg cgctcatgtc gatgccctgg caaccggcga
     6841 tccggctcgc acccgcctgg ggcgacgatc ccgtgtacat ccgtgcgctt gcccaatcga
     6901 tccgccacca tctcggcacg cttgatttcg agcccgagcg gctcgtcgtc acctatcacg
     6961 gcatgccgca gcgctatctc ttgcagggtg acccctacca ttgccagtgc ctgaaatcct
     7021 cgcgcctgct agcggccgag ctcggctggc cggagggccg catgttgacc tcgttccagt
     7081 cccgcttcgg caccgaggag tggctgcgac cctacaccga cgagacgatc accggcctcg
     7141 ccaaatcggg cgtgaagcgc atcgcagtcg tagcccccgg cttcaccgcc gactgcctcg
     7201 agacactcga ggagctcgac gtcgaaaacc gcgggttctt cacctcgaac ggcggcaccc
     7261 acttcagcta catcccctgc ctcaacgaca gcgaccccgg tctcgacgtg atcgaggcgg
     7321 tggtcaggcg agagctcgcg ggctgggctt gagggggcga tcgagccgcc tcgcaagtgc
     7381 atccgctacc gcgcgagcgc ctgcgcgaga tcagcaacga ggtcgtcggg atgctcgatt
     7441 ccgaccgaca ggcggatcgt cgtctccagg acaccgatgc gctggcggac gtcgaccgga
     7501 atgcccgaat gcgtcatcgt cgccggatgg ctcgcaagcg actcggtacc gccgagactg
     7561 acagcgagct tgaacacctg cagcgcattg aggaaggcga aagcctccgc ctgcccgccc
     7621 ttgatgtcga acgagaacgt cgaaccggcg cccgtacatt ggcgctcgta ggtcgcgcgc
     7681 tcggcaccgg cgccaaggaa cggcagatag tgcacctttc cgacgcgcgg atgatcgcgc
     7741 aggaactccg cggcgatttt cgcgttggcg ttcgccttct ccatccggat gctgagcgtt
     7801 tcgagcgagc ggccgagcat ccagcacgaa tgcggatcga gctgtgtacc gatcgcgctg
     7861 cgcagcgcct tcacggggcc gattgccgat ttcttcccga gcgcggcgcc tgcaatcaga
     7921 tcagagtgcc cgccgacgta tttcgtcagc gaatagatcg acaagtcggc gccgaaatcg
     7981 agcgggtgct ggaacacggg gccgaggaga gtgttgtcgc acgcgatgat cggtcgatgc
     8041 gcttgacttg cgccgaggtc gtcggtcaga cggcgcacga gtgcgatatc gaccagcgtg
     8101 ttggtcggat tggaaggcgt ttcaacgaga accacggcaa tgcggcccgc ctgctttgcc
     8161 cgatcgagtg ccttgcggat cgttgtttca ctgacgccat cggagaacgg caccgatctg
     8221 acgccgaact cggcaaacgt tctggtcagc agagtttccg tgccgccata gagaggttgt
     8281 gagtgtacga tcacgtcgcc cggccgcgcg taggccatca gcgtcgtcgc tatggccgac
     8341 atacccgacg agaacaggat ggcagcctcc gcgccttcat agattgcaag ccgatcctcg
     8401 acgatttcgc tgttcggatg gttgaacctt gaatagacga gaccggcgcc cgcacccgca
     8461 ggcggcgtct tgcgccctga cacatagtcg aagaaatcac gcccttcctc cgccgttccg
     8521 aacacgaatg tcgaggtgag gaacaccggc ggcttgacgg ccccttccga cagcatcgga
     8581 tcgaagccgt agccgagcat cagcgtctca ggtttcagca cgtggttgcc gatgtgcgtt
     8641 ttcgaagggc gtggggcggc catggccatg atcctgcgat tggtttgcga taccggcaat
     8701 ttagctgcga ctctgccgca tcaatgtgct attttctgcg gcaatagccc aatggtcagc
     8761 agataaattt acaagatcga cgtgagccag cagaaactgc ctatgattct tgacgacgtc
     8821 gacaggcgca tcctgcatgt gctcgccggc aatggccgca tcacgaacca agagctcgcc
     8881 gataaagtcg gcctgtcccc ctcaccttgc ctccgacggc tgcgccgtct cgaagacatc
     8941 ggtgtcatca aggggtacac agtcctcgtc gatccgaaag ctcacggttg gttcatgtcc
     9001 gcgatcgcca cgatccgctt gacccgtcag aacgaggacg acatcgagat gttcgagcgg
     9061 gcggtgcagg cgtggcccga agtgtccgag tgctatctcg tgaccggctc acgcgactac
     9121 atcctgaaaa tcatggcctc cgatctcccg gactacgagc gcttcatcaa gggccgcatt
     9181 gcacggctga agtgcgttgc ttcgatcgag accagcttca tcatgaacac gatcaagggc
     9241 cgcggttgat cgggcgcaca gccccgttgt cttggtcggg caaaattgct taggactggc
     9301 agtccagtct cgagagaccg cccatgaacc tcgcgcacac cctgcaggtt gcagccctcc
     9361 agtatcccga ccgccccgcc gtatcggagg aggggcgcac gctcgactac ggggccatgg
     9421 aggtgctcgt cgggcgcatc gcgggcgggc tcagggcgcg gcatggactc aagcctggcg
     9481 accgcgtcgc cttgtggatg gacaacacgc tcgaggtcct gccgctgctc tacggcatct
     9541 ggcgcgcagg cctcacggcc gtgccggtca acgcgaagct gcacgccaag gaactcgctt
     9601 ggatcgtcgc cgactcgggt gcgcagctcc tcttcgtcac cgacgccctt ggtgccaagc
     9661 tctccgagct gccgtccggt gcgagcctgc cccgcgtgat tgccgcgact gccaaagaaa
     9721 tcgccgcact tgcgacggcg gacccattga cgcgcgggcc gaccgacccc tcggacgagg
     9781 cctggctttt ctatacgagc ggcacgacag gacggccgaa gggcgccgtg ctcacgcacc
     9841 gcaatctcct cttcgccgtg catgcctact acgccgacat cgacttccta ggaccgagcg
     9901 acaccatcct gcatgcagcc cccctatcac acggctcggg gctctacggc ctcgcctttg
     9961 tcgcgcgcgg cggccacaac gtgatcatcc ccggatcgtt cgagccggat cgcatctaca
    10021 gggcaatagc agagcactcg gacgtctcgt tcttcgccgc gccgaccatg gtctcgcgac
    10081 tgatcaacca tccgctcgcc ggcagcaccg acacgcagaa cctgaagacg atcagctatg
    10141 gcggcgcgcc gatgtacgtg gccgacctcg agcgcgcgct cgagctcttc ggaccgaagc
    10201 tcttccagct ctacggccag ggcgagagcc cgatgacgat cacggggctc ggcaaggcgg
    10261 cgcacgccgc tcgccaccgc gctaccgcga gcggctcggc tcgacaggtg tcgcgcgcac
    10321 cggcgtcgct ttccgcgtgg tgggcgacga cggggccgag ctgccgccgg gcgagatcgg
    10381 cgagatcgtc acacgaagcg actgcgtgat gaaaggctac tggaacaacc cggaggcgaa
    10441 tgcgaaggcg atccgcgatg gctggctctg gaccggtgac atgggcgccg ccgacagcga
    10501 cggcttcctc accatcaagg accgctccaa ggacatgatc atctccggcg gctcgaacat
    10561 ctatccgcgc gagatcgagg aggttctggc ccgccacccc gccgtgctcg agtgcgcggt
    10621 cgtcgggcga atacatcccg aatggggcga ggaggtcgtg gcgttcgtgg tggcgcgccc
    10681 tggcacgagg atcacagccc cggagctcga ccggctctgc ctcgacaata tcgcgcgctt
    10741 caagcggccg aaggactacc gcttcgtcga cgccctgccg aagaacaact acggaaagat
    10801 cctgaaaact gagcttcgcg aagtctttcc ggaaaagaaa tcctgacttg gatcagatga
    10861 cgctgatatg gttcggtcgt caactcatca ctgaatgcca gtccgggagg acgacttgac
    10921 caatacatcg atcggaaatc tggccgccgg cttcatcgcg gctgcgctct ctgtcctgct
    10981 catccaccag ggcatgcaat gggtgctgac gcagacgggc ctcgtcaaac tcgagccgtg
    11041 gtcgttcaag ccggtggggc cgttctccgt gccgacgctt gtcaactcga tcttctgggg
    11101 cggtctgtgg ggtctcctgt tcgcgctcgt ctgggacaag gtcccgggct cggcgatgtg
    11161 gatcaagggc ctcatctacg ggttcgtcat tctgctgatc tcgaactgga cgctgcttcc
    11221 gctcatcaag ggcaagatct tcggccaggc caatcaggcg ctctttgcgg gcttcgtgcc
    11281 ggcccgcatg ctggcaggcc tcctcattct cggcgcattc ggcgctggca ccggcgccat
    11341 ctacagcctg ctcaggagcc gcgactgacg ggcgcccgga gagcgcttgc gagtatctga
    11401 cccgcgtgct ctccgcttga cggattggcc ctgaccggcg ccaagtcact gcatcaagag
    11461 accagatcag tggagcaggc acgcatggcc aacaagattg atctcgccgg caagatcggc
    11521 gtcgtgaccg gcggtgcgca aggcatcggc cgtgccgtcg tcgagcggtt catcgcctcg
    11581 ggcgccaagg tcgcgatctg ggatttcgac aaggcgctcg ctgagaaatt cgccaaggag
    11641 gcgggcggcg gcacgcttgc cgttgcttgc gacgtcactg atcccgcgag cgtggacgcc
    11701 gcggccaagg cgaccgaaaa ggccttcggg cgcatcgnnn nnnnnnnaca acgccggcat
    11761 cgccgggccg acggccaagg cctgggagta tcctctcgct gagctccggc gaacgatggc
    11821 cgtgaacctc gacggcccgt tcaactgctg ccacgccgtc gtgccgggca tggtctcacg
    11881 caactacggc cgcatcgtca acatcgcctc gatcgccggc aaggaaggca accccaacgc
    11941 gtcggcctac tcggcctcaa aggccgcact catcggcttc acgaagtcgc tcgccaagga
    12001 gcttgcgacg cacgacatcg ccgtcaatgc catcgccccg gcggttgccc gcacagcaat
    12061 actcggacag gtgacgcagg cgcacatcga ctacatgatc tcgaaaatcc cccgcggccg
    12121 cacggtcgag ccgagcgaga tcgcggcgct gatcgcctgg gccgcctccg ccgactgctc
    12181 gttcaccacg ggcagcgtcc tcgacatctc gggcggccgc gcgacgtact gataccgggc
    12241 gcgcccgccg tcagccttgg cggcgggcct tgatggcagc ggcgagcgtt ccctcgtcca
    12301 gatagtcgag ctcgccgccg atcggcacac cttgcgcaag ccgcgagacg attacgccgt
    12361 tgccggagag ctgttcggca atgaagtggc tcgtcgactg accttccacc gtctggttga
    12421 gcgccagcag cacctcgcgg acgaccggcg gtcgcgcgcg ctcgatgagc ttcgcgatgt
    12481 tcagacgttc gggcccgatg ccatcgagcg gcgagaggtg gccgccgagc acgtggtagc
    12541 gggcattgag cacggccgcg cgctcgagcg cccacagatc gccgacctcc tcgacgacga
    12601 cgatgagact ctggtcgcgg cgcgggtcgg cgcagacagt gcagggtgca ctcgtatcca
    12661 cattgccgca ttcggcgcag atggtgagcc ggtcggcggc cgaggcaagc gccgccgcga
    12721 gcgggcgcag gaggtcgtcg cgccgcttga tgagggtcag cgcggccttg cgcgccgagc
    12781 ggggcccgag ccc
//
LOCUS       DAORNH010000111        12736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3721_length_12736_cov_2.634414, whole genome shotgun sequence.
ACCESSION   DAORNH010000111 DAORNH010000000
VERSION     DAORNH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_12736_cov_2.634414"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..616)
                     /locus_tag="PK264_12450"
     CDS             complement(26..616)
                     /locus_tag="PK264_12450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD76728.1"
                     /translation="MSPTVEPLTGDALASALPALARLRIAVFRDWPYLYDGTLAYEEG
                     YLRKFSSSKDAVIVAARDQGEIVGVATGSPLRDHAAEFAKPFEAAGHDIDRIFYFGES
                     VLLPSYRGRGIGHRFFDAREAHARALDRFAHTTFCGVVRPPEHPAKPAGYRPLDAFWE
                     SRGYRRIEGLTAEFTWKDLGAEEPTAKHMQFWMRAL"
     gene            complement(630..1046)
                     /locus_tag="PK264_12455"
     CDS             complement(630..1046)
                     /locus_tag="PK264_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HRD76729.1"
                     /translation="MSAETLSLIRRYYDAFNRGDATGMLACVSDDVVHDVNQGMRRTG
                     REAFAAFCKHMERCYKEELLDIVLFATPDGRRAASEFNVRGTYLETDEGLPPAKGQTY
                     LLPAGTFFAVRDGRISRITTYYNFTDWLLQVGVPER"
     gene            complement(1137..2390)
                     /locus_tag="PK264_12460"
     CDS             complement(1137..2390)
                     /locus_tag="PK264_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD76730.1"
                     /translation="MRNTHVVWLSAAALPLLAATAVAQTKGPAPSGPPPVVAPAGAPT
                     APAAAPKGPTAVSYEEALLSAANNLLSKAVLPPGDKKLQVVIDPLIDGQTGHVSPTTQ
                     RIGRRITDLIKTNYPRLEVVAFTPENVAKKPLLLIGTFTAINLAGKADAPKDAYRLCL
                     ALGDLNTGKLVSKGVGFMKAEQISVAPTPSFADAPVWTKDKSIEGYVKTCQGTKVDDP
                     INAAYVDRIATAAMIADAMAAYDAKRWPDSLKRFQAALAAPGGDQLRVHNGIYLANWK
                     LGRKKEAAEAFAKVIDHGLTTDRLAVLYLFRPGSTSWAAVKGGLPPYAMWTDQIAKRT
                     EAKKACLEIVGHSSRSGNEDMNVRLSGRRADLIRDRLVRVIPALNTRVIASGVGSKEA
                     VVGTMPDSAANNIDRRVEFKVAKCS"
     gene            complement(2489..3289)
                     /locus_tag="PK264_12465"
     CDS             complement(2489..3289)
                     /locus_tag="PK264_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HRD76731.1"
                     /translation="MSSISGRPDPVVRTITRSDIVEALARGLRDFQAAPRFGLIFGGL
                     YALGGIAILLCLSLLQMTYFAYPIAAGFAILGPSIALGLYQVSRDLESGTATSFGRIW
                     SVVKSRREVGWMAFVTGFIFVIWMYQVRLLLAVVLSNAGSFTSLREFIEIVLTTNEGL
                     VFLLLGNVIGAVLSLLLFSLTVVSFPLILDRDVDFVTAMITSVRAVAASPLPMIGWAA
                     VIVMLLVVSALPLFLGLVITLPILGHATWHLYRRIVLPAEPAVATATK"
     gene            complement(3368..4566)
                     /locus_tag="PK264_12470"
                     /pseudo
     CDS             complement(3368..4566)
                     /locus_tag="PK264_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            complement(4600..>5744)
                     /locus_tag="PK264_12475"
     CDS             complement(4600..>5744)
                     /locus_tag="PK264_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HRD76732.1"
                     /translation="NSAPALQQGSAMKITDVTIRIFAWDGIPATQYGRHTGAFSGKSD
                     LGLVTIATDQGLEGHAFLGSSSRPASNDAITIIRALKPLLIGEDPLDRERLFERMWTR
                     MRVGSLRCLGACDVALWDLAGKIAGLPVHKLMGSYRRSVPAYASSAVLGEIEAYCEEA
                     KSFKAAGWPAYKIHPPTLWEKDIKVCEAVRKAVGDDYRLMLDSTWAYRYPEALRVGQA
                     AERLGFYWYEDPLAEDDLTNCIKLRQKLAIPLMATEYSPGAHTAYAPWLAFQATDYLR
                     GDVAVKGGLTACLKTAHIAEGFHMNFEVHHGGNSLNNFANLHLIMAIRNCEYFEVLLP
                     SGAQKYGILNDIEVDAHGMVHAVDTPGLGAMIDFDLIKRKEVAILA"
     assembly_gap    5745..5757
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5758..6184)
                     /locus_tag="PK264_12480"
     CDS             complement(<5758..6184)
                     /locus_tag="PK264_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76733.1"
                     /translation="MRLSSASVAVVLALAGLSAVPPASAQTLSCPRADFETVVEAASA
                     ALRDLNAKNKPRFQSELRELMKKRGWAPDQLMREAAPIVQDDKVAAYDKRTSELLDKI
                     NAMATGTSSDCVALAELKGHMAALVETQQAKWAYLFERLA"
     gene            complement(6285..>6724)
                     /locus_tag="PK264_12485"
     CDS             complement(6285..>6724)
                     /locus_tag="PK264_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HRD76734.1"
                     /translation="GLYQAAMASRFVDELPADHVDVIEAKSPFGGVYANLGRSNPFGR
                     SRFDEQPQASYQTPGWQRAQQRWSSPSGQSQRPSRGPLTIDGELVASSTGREASYVVG
                     DRVLHQKFGPGRVTEVDGNKLTIEFERAGRKRVVDSFVERMAG"
     assembly_gap    6725..6734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6735..8682)
                     /locus_tag="PK264_12490"
     CDS             complement(<6735..8682)
                     /locus_tag="PK264_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRD76735.1"
                     /translation="MITNRQSDADWTAADGPQGPDGHQALSISARALARTAPSYLDGL
                     NPEQRKAVETLDGPVLVLAGAGTGKTRVLTTRIAHILASGRCRGSEILAVTFTNKAAR
                     EMKERIGKLIGGAVEGMPWLGTFHSIGVKILRRHAELVGLKSGFTILDTDDQIRLVKQ
                     VIEADGLDKDRWPGRQLAALIDSWKNRGLTPDKVPSGENFAFGNGRGAKLYAAYQTRM
                     KELNAVDFGDLLLESLRLFAGHPDILADYQRRFRYILVDEYQDTNVVQHLWLRLLAQG
                     SPNICCVGDDDQSIYGWRGAEVDNILKFERDYPGATVIRLERNYRSTGHILGAASRLI
                     AANKSRLGKTLHTDDQLGQKVQVTGVWDDEEEARQIGEEIERLGKSGHRLNQIAVLVR
                     ASFQMRAIEDRFVTLGLPYRVVGGPRFYERQEIKDAIAYLEITLNPANDLKFERIINV
                     PKRGLGDTTVARIHDLARARQVPMAIAAREIAETEELAAKARRSLRDLMLSFERWRGH
                     VPTMKHTDLAELILDESGYTAMWQADKSPQAQSRLENLKELIRFMHQFETLPGFLEHV
                     SLVMDTETGDDGDRVSLMTLHAAKGLEFDTVFLPGWEEGLFPHQRSLDENGVAGLEEE
                     RRLAYVGLTRARRRAIVSFTQNRRN"
     gene            8819..9064
                     /locus_tag="PK264_12495"
     CDS             8819..9064
                     /locus_tag="PK264_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HRD76736.1"
                     /translation="MLKLRPNCECCDRDLPPESAEAVICSFECTFCRDCASTRLPGGR
                     CPNCGGELVKRPVRPADRLAKFPASSERVKKAHAFCA"
     gene            9179..10237
                     /gene="gmd"
                     /locus_tag="PK264_12500"
     CDS             9179..10237
                     /gene="gmd"
                     /locus_tag="PK264_12500"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRD76737.1"
                     /translation="MTRRALITGVTGQDGAYLAELLLAKGYEVHGIKRRSSSFNTQRV
                     DHLYQDPHEGDVPFLMHYGDMTDSTNLIRLVQQIRPSEIYNLAAQSHVAVSFESPEYT
                     ANADAVGVLRLLEAIRILGMEKDCRFYQASTSELYGLVQEVPQRETTPFYPRSPYAVA
                     KLYGYWITVNYREAYAMHASNGILFNHESPIRGETFVTRKITRAVARIEVGLDQQLYL
                     GNLDAKRDWGHARDYVEGMWRILQQETPDDYVLATGETRSVREFVEIAFAHVDKRIVW
                     RGKGVEESGVDQRTGKVLVKIDPRYFRPTEVDLLIGDPAKAREKLGWTPQTSFAGLVK
                     EMMDGDLAAARREASNVR"
     assembly_gap    10313..10460
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10461..11175
                     /locus_tag="PK264_12505"
     CDS             <10461..11175
                     /locus_tag="PK264_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRD76738.1"
                     /translation="NNTLRAEFLYDNLAIATNIIHASHRQGVEKLMFLGSTCIYPRLA
                     PQPMNEDALLTGPLEPTNEPYAIAKIAGIKMCEAYRAQYGSDFISVMPTNLYGPGDNY
                     HPEHSHVVAALIRRFHEAKLSGAGEVVVWGTGTPRREFLYVDDMADASVFAMKRYSGA
                     GILNIGTGEDIAISEFARLVAKVIGYTGRIAYDTSRPDGTPRKLVDVARMSALGWRAS
                     TGLDAGLARAYQSFLESLK"
     gene            complement(11206..11970)
                     /locus_tag="PK264_12510"
     CDS             complement(11206..11970)
                     /locus_tag="PK264_12510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76739.1"
                     /translation="MPTKLFPGERALVTGSASNIGRAIATALAREGAHVILADVDPKR
                     NAEVGAEIKAAGGSASEITCDLSKPDGWKPLLEVARAPVVSMLVHSASPARREADHTL
                     AVTEEVWDGMINTNLRSGFFLARGIGLAMRDAGIKGRLLLITSLHAETPRNLPHYSAS
                     KAGMTMVVKELARALGPSGIRVNALAPGAVPGGGFNASAFSFDGMIPLGRLGAAADMA
                     ATATALLSDRFSAYVTGTTVEVDGGIKGHNWIAPTR"
     gene            complement(12094..>12736)
                     /locus_tag="PK264_12515"
     CDS             complement(12094..>12736)
                     /locus_tag="PK264_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76740.1"
                     /translation="IADDAGPAPAHAHPAPTLPAPASPAPWAMPPADSPRVLTFASWP
                     SEPTVTVLLVMQPGSRGIRRHNKTADPILCADHGCYISRGSDTPATLRSGRKAFGFLN
                     TFGGRAGACRDQLGCVFRGVRLDGPEATLQPVDMRLVHHDRREAQSITSDSACRMERG
                     ALACARAIASADYVMWIVPEALAENAGAERLEAAVAAGLAATSSTARADVSRR"
BASE COUNT         2093 a         4109 c         4201 g         2162 t
ORIGIN      
        1 gatgcgaacg cggtgctcac tcatctcaca gcgccctcat ccagaactgc atgtgcttgg
       61 cggtcggctc ctcggcgccg agatctttcc acgtgaactc ggccgtgagg ccctcgatgc
      121 ggcggtagcc gcgggactcc caaaaggcat cgagcggccg gtatccggcg ggttttgctg
      181 ggtgttccgg tggccgaacc acaccgcaaa aagtcgtgtg tgcaaaccgg tccagcgcac
      241 gcgcatgcgc ctcgcgcgcg tcgaagaagc ggtgtccgat gcctcgccca cgatagctcg
      301 gcagcagaac cgactccccg aagtagaaga tgcggtcgat gtcgtggccg gctgcctcga
      361 acggcttggc aaactcggcc gcgtggtccc ggagcggcga tcccgtcgcg acgccaacga
      421 tctctccctg gtcgcgtgcg gcgacgatga ccgcgtcctt ggagctcgag aacttcctga
      481 gatagccttc ctcgtaggcg agagtgccgt cgtagaggta cggccagtca cggaagacgg
      541 cgatcctgag ccttgccagc gctggcagtg cagaagcgag ggcgtcaccg gtcagcggtt
      601 cgacggttgg cgacatgaaa gcaatcccat tacctctcgg ggacgccgac ttgcagcagc
      661 cagtcggtga agttgtagta ggtcgtaatc cggcttatgc ggccatcgcg cactgcgaaa
      721 aacgtgccgg cgggcagcag atacgtctgg ccttttgcgg gcggcaggcc ctcgtcggtc
      781 tcgagatacg tgccgcgcac gttgaactcg ctcgcagcgc gtctgccatc cggagtcgcg
      841 aacagcacga tatcgagcag ctcctccttg tagcagcgct ccatatgctt gcagaacgcc
      901 gcgaaggcct ccctgccggt gcgccgcatg ccctggttga cgtcgtggac gacgtcgtcg
      961 ctgacgcacg ccagcatgcc ggtcgcgtcg ccgcgattga aggcgtcgta gtagcggcgg
     1021 atgaggctca gggtctcggc ggacatgcat gcggctccga aagtgatcag gggccgtctt
     1081 gcgacagccc ccggtgaaaa tcaacactga cgtggtgcta tcgttggtgc gccggatcag
     1141 ctgcacttcg cgaccttgaa ctcgacgcgt cggtcgatgt tgttggccgc gctgtccggc
     1201 atcgtgccga ccacggcttc cttggagccg acgcccgacg cgatgacgcg cgtgttgagc
     1261 gccgggatga cgcgcacgag gcgatcgcga atgaggtcgg cgcggcggcc cgagaggcgc
     1321 acgttcatgt cctcgttacc cgatcggctc gaatggccga cgatctcgag gcaggccttc
     1381 tttgcctccg tgcgcttggc gatctggtcc gtccacatgg cgtagggcgg caagccgccc
     1441 ttcacggcgg cccaagatgt cgatccgggc cggaagagat agagcactgc gagacgatcg
     1501 gtggtcaggc cgtgatcgat gaccttggcg aaggcctcgg ccgcttcctt cttgcggccg
     1561 agcttccagt tggcgaggta gatgccgttg tggacgcgga gctgatcgcc gcccggcgcc
     1621 gccagagcgg cctggaagcg cttcagcgaa tccggccagc gcttggcatc gtaggcggcc
     1681 atggcgtcgg cgatcatcgc ggcggtggcg atgcgatcga cataggcggc attgatcgga
     1741 tcatcgacct tcgtgccctg gcatgtcttc acgtagcctt cgatgctctt gtccttggtc
     1801 cagaccggcg catcggcgaa cgagggcgtc ggcgccacgc tgatctgctc ggccttcatg
     1861 aagccgacgc ccttggagac gagcttgccg gtattgagat cgccgagtgc gaggcagagg
     1921 cgataggcgt ccttcggcgc atcggccttt ccggcgaggt tgatcgccgt gaaggtgccg
     1981 atgagcagga gcggcttctt ggcgacgttc tccggcgtga aggcgacgac ctcgaggcgc
     2041 ggatagttgg tcttgatgag atcggtgatg cggcggccga tgcgctgcgt cgtcggcgag
     2101 acgtggccgg tctggccgtc gatcaacggg tcgatcacca cctgcagttt cttgtcgcct
     2161 ggtggcagga cggccttcga gagcagattg ttcgcggcgc tgagcagcgc ttcctcatat
     2221 gacaccgccg tcggcccctt gggggctgca gccggtgcag ttggtgctcc cgctggcgcc
     2281 actacaggtg gtggcccgct cggcgcaggc cctttggtct gcgcgacggc tgttgcggcg
     2341 agcaacggca gtgccgccgc cgacagccaa acgacatgag tattccgcat ctccagtgcc
     2401 tccgtatgaa atcccagtag gcacttcctt agcctaaaat gcggttgccg gcaaaatgct
     2461 ccgtaaccgc cgcgatacct gttggccatc attttgtggc agtagcgacc gcgggttcgg
     2521 ccggcaaaac gatccggcga tagaggtgcc acgtcgcatg gccgaggatc ggcagcgtga
     2581 tgacgaggcc gagaaatagc ggcaacgccg agacgaccag cagcatcacg atcaccgccg
     2641 cccagccgat catcggcagc gggctcgcag ccacggcccg cacgctcgtg atcatcgccg
     2701 tgacgaagtc gacgtcgcgg tcgagaatca aggggaagga gaccacggta agcgagaaca
     2761 gtaggagcga cagcaccgcg ccgatcacgt tgccgagcag caggaagacg agaccctcgt
     2821 tggtcgtgag cacgatctca atgaactcgc gcagcgatgt gaaggagccc gcgttgctca
     2881 gcacgacggc gagcagcagt cgcacctggt acatccagat gacgaagatg aaaccggtga
     2941 cgaaggccat ccagccgacc tcgcgccggc tcttcacgac cgaccagatg cggccgaacg
     3001 aggtcgcagt accgctctcg agatcgcggc tcacctgata gaggccgagc gcgatggacg
     3061 gtccgaggat cgcgaagccg gcggcgatcg gataggcgaa gtaggtcatc tgcagcagcg
     3121 acaggcagag aaggatcgca attccgccga gcgcgtaaag cccgccgaag atcagcccga
     3181 agcggggtgc ggcctggaag tcccgaaggc cccgggccag cgcctcgaca atgtcgctgc
     3241 gagtgatcgt ccgtacgacg ggatccggcc tgcctgaaat tgacgacatc gtgcctgctc
     3301 cccggagttg ccgccgcaaa acaggccgac gctctcacaa ctccgccttg atacaggtca
     3361 atgccgctca gccgatgccc tcctcccacg gcacgatcgt gctcttcacg gtcttcatgg
     3421 ccatggcgtc ggcgacggcg cgcttcacgc cgtcgagcga gaagggatag agcgtctgca
     3481 tcctgcgcca ggggtactta ttgcgtgcct tgtcgagcat ctcgacgccg agcacgaggt
     3541 cgttcgcggt gaaggcccac gagccgagca gattgatatc cttcgtgcag atgcggtgcc
     3601 agctggtcat gatggagccg gcgtcggtga actggcccat ctccacgtac gtgccaccgt
     3661 cgcgcaggaa ctcgatgccc tccgggccag cggtcgggtg tcccgagcag tccatgacga
     3721 gatcggcgcc atagccgccg acgatgtcgc gcacggcctt gatgcgcgcc tccgccgtgc
     3781 ggtgctcgtc gatgttgacg gttgcctcgg cgccgaactc gcgggcgagc ttcaggcgcg
     3841 gctcctcggg cgcgccgacc gcaatcacgc gcccatttcc tgcgcggcgg cgatggcgag
     3901 gatgccgatc ggcccggtcc cctggatgac gaccgtatcg ttccacttga agccgcccgc
     3961 tcgcgccgca cggttgaagg cccggatgca ggaggtgagc ggctccgaca gcgtgccgag
     4021 caggagactc atgtcgtcgg ggagcttgta gatcttcgtc cctggcagct cgccgagatc
     4081 gacgtagaca tactcggccc atccgcccca cagatgcggg ggcttctcga agccgagata
     4141 gcggccgtag tagaccggag tcaggcactt gttcgactgc tcgggatact tcttacacca
     4201 gtcgcagaag ccgcacggca tgagcggcgg cagcatcacc ttggagccga ccttcagggg
     4261 cttccccgag tgatccgcct tcagctcggc gccgatctcg acgatgacgc cggcaagctc
     4321 gtggccgagc gtgaagggcc agggcagcgg cttcggccaa tggcccttga ggatgtgctg
     4381 gtcggtaccg cacacgccgc aggcgccgat ccggatgagc gcggccttgg gcggcacatt
     4441 cggccaaggc acctcgcgga tcaccggctc agcgcccggc ccgtcatagg tcgcgacgcg
     4501 gatcttctgc ttgccggcag gcaccggctt cgcggctttc gacgcagctt ttgttttggt
     4561 ggccatgctt gagctcagct gttttcggtc tctctggggt caggcgagga tggcgacttc
     4621 cttgcgcttg atgaggtcga aatcgatcat ggcgccgagg cccggcgtgt caacggcatg
     4681 caccatgccg tgggcgtcca cctcgatatc gttgaggatg ccatatttct gcgcgcctga
     4741 gggcagcagc acctcgaaat actcgcagtt gcggatcgcc atgatgagat ggaggttcgc
     4801 gaagttgttg agcgagttgc cgccgtggtg cacctcgaag ttcatgtgga agccctcggc
     4861 gatgtgcgcc gtcttgaggc acgctgtgag cccgcccttc acggcgacgt cgccacgcag
     4921 gtagtcggtc gcctggaagg cgagccacgg cgcgtaggcg gtatgagcgc ccggcgagta
     4981 ctccgtcgcc atcagcggga tcgcgagctt ctggcggagc ttgatgcagt tggtgagatc
     5041 gtcctcggcg agcgggtcct cgtaccagta gaagccgagc cgctctgccg cctggccgac
     5101 gcgcagcgcc tccggatagc gataggccca ggtcgaatcg agcatcaggc gatagtcgtc
     5161 gccgaccgcc ttgcgcaccg cctcgcacac cttgatgtcc ttctcccaga gcgtcggcgg
     5221 gtggatcttg taggccggcc agcctgccgc cttgaaggac tttgcctcct cgcagtaggc
     5281 ctcgatctcg ccgagcacgg ccgaactcgc gtaggccggc acactgcggc ggtagctgcc
     5341 cataagcttg tgaacgggga gacccgcgat cttgccggca aggtcccaga gcgccacgtc
     5401 gcaggcgccg agacagcgca gcgagccgac ccgcatgcgc gtccacatgc gctcgaacag
     5461 ccgctcgcgg tcgagcgggt cctcgccgat gagcagcggc ttcagggcac ggatgatggt
     5521 gatggcatcg ttgctcgccg ggcggctcga cgagccgaga aaggcatgcc cctcgaggcc
     5581 ctggtcggtc gcgatcgtga cgaggccgag gtcgcttttc cccgagaacg cgccggtgtg
     5641 ccggccgtac tgcgttgccg ggataccgtc ccaggcaaaa atgcggatcg tgacgtcggt
     5701 gatcttcatg gcgctcccct gctgcagggc cggagcgcta ttttnnnnnn nnnnnnnccg
     5761 cgagcctctc gaaaagatag gcccatttgg cctgctgtgt ctcgacgagt gcggccatgt
     5821 gcccctttag ttcggcaagc gccacgcaat ccgagctcgt ccccgtcgcc atggcattga
     5881 tcttgtcgag gagttcgctc gtgcgcttgt cgtaggccgc gaccttgtcg tcttggacga
     5941 tcggcgccgc ctcgcgcatc agctggtcgg gtgcccagcc gcgctttttc atgagttcgc
     6001 gcaactcgct ctggaagcgc ggcttattct tggcgttgag gtcgcggagt gctgccgatg
     6061 ccgcctcgac caccgtctcg aagtcggcgc gcgggcagga cagcgtctgg gccgatgcgg
     6121 gggggactgc agagaggccg gcgagcgcga ggacaaccgc cacgctcgcg ctgctcaatc
     6181 gcatcagccg aattctcgtg cccgtcctca tgccgcccgg ttccaccctc tacgccgcca
     6241 ccaagccgta tgctcctaca gcagaagatc acgttccacg ccgatcagcc agccatgcgc
     6301 tcgacgaagc tgtcgacgac gcgtttgcgt cctgccctct cgaattcgat cgtcagcttg
     6361 ttgccgtcaa cctcggtcac gcggccgggg ccgaatttct ggtgcagcac ccggtcgccg
     6421 acgacatagc tcgcctcgcg gcccgtagac gacgcaacga gctccccatc gatcgtgagt
     6481 ggcccccgcg acgggcgctg ggactggccc gagggcgacg accagcgctg ttgcgcccgc
     6541 tgccacccgg gcgtctggta gctcgcctgt ggctgctcgt cgaagcggct gcggccgaag
     6601 ggattgcttc ggccgaggtt cgcatagacg ccgccgaacg ggctcttcgc ctcgatcacg
     6661 tcgacgtggt cggccggcag ctcgtcgacg aagcgcgagg ccattgccgc ctgatagagc
     6721 ccgcnnnnnn nnnngattgc gccggttctg cgtaaaggag acgatcgccc gccggcgggc
     6781 gcgcgtgaga ccgacatagg cgaggcggcg ctcctcctcg aggccggcga cgccgttttc
     6841 gtcgaggctc ctctggtgcg ggaagagccc ctcctcccag ccgggcagga acaccgtatc
     6901 gaactcgagc cccttcgctg cgtgcagcgt catcagcgag acgcggtcgc cgtcgtcgcc
     6961 tgtctccgta tccatcacca acgagacgtg ctcgaggaag cccggcagcg tctcgaactg
     7021 gtgcatgaag cggatgagct ccttaaggtt ctcgagccgc gactgcgcct gcgggctctt
     7081 gtcggcctgc cacatggccg tatagcccga ctcatcgagg atcagctcgg cgaggtccgt
     7141 gtgcttcatg gtcggcacgt ggccgcgcca gcgctcgaag gagagcatca gatcgcgcag
     7201 cgaccggcgg gccttggcgg cgagctcctc cgtctccgcg atctctcgtg cggcgatcgc
     7261 catcggcacc tgccgggcac gggcgagatc gtggatgcgc gcaaccgtcg tgtcaccgag
     7321 gccgcgcttc ggcacattga tgatgcgctc aaacttgagg tcgttcgccg gattgagcgt
     7381 gatctcgagg taggcgatcg cgtccttgat ctcctggcgc tcgtagaagc gtgggccgcc
     7441 gacaacgcga tagggaaggc caagcgtcac gaagcgatcc tcgatcgcgc gcatctgaaa
     7501 ggaagcccgc accagcactg caatctgatt gagcctgtgc ccgctcttgc ccagccgctc
     7561 gatctcctcg ccgatctggc gcgcctcctc ctcgtcgtcc cacacacctg tgacctgcac
     7621 tttctggccg agttggtcgt cggtgtgcag cgtcttgccg agccggctct tgttggcggc
     7681 gatcaggcgc gaggcggcgc cgagaatgtg gccggtcgag cgatagttgc gctcgaggcg
     7741 gatcacggtt gcgcccggat agtcgcgctc gaatttcagg atgttgtcga cctccgcgcc
     7801 gcgccagccg tagatcgact ggtcgtcgtc gccgacgcag cagatgttgg gcgagccctg
     7861 cgcaaggagg cggagccaga gatgctggac gacattggtg tcctggtact cgtcgaccag
     7921 aatgtagcgg aaacggcgct ggtagtcggc gagaatgtcc gggtgaccgg cgaagaggcg
     7981 caggctctcc agcagcagat cgccgaaatc gaccgcattc agctctttca tgcgcgtctg
     8041 gtaggccgca tagagcttgg caccgcggcc gttgccgaag gcgaagttct cgccgctcgg
     8101 caccttgtcg ggcgtgaggc cgcggttctt ccaggaatcg atgagggccg cgagttgccg
     8161 tcccggccag cggtccttgt cgagcccgtc ggcctcgatg acctgcttga cgaggcggat
     8221 ctggtcgtcg gtatcgagga tggtgaagcc ggatttcagg ccgacgagct cggcgtggcg
     8281 ccgcaggatc ttcacgccaa ttgaatggaa ggtgccgagc cacggcatgc cctcgacggc
     8341 gccgccgatc agcttgccga tccgctcctt catctcgcgg gcggccttgt tcgtgaacgt
     8401 gacggcgagg atctccgagc cgcggcagcg gcccgacgcc aggatgtggg cgatgcgggt
     8461 agtgagcacg cgcgtcttgc ccgttccggc gccggcgagc accagcacgg gaccgtcgag
     8521 cgtctcgacg gccttgcgct gctccggatt gaggccgtcg agatatgatg gcgcggtccg
     8581 ggcgagcgca cgtgcggaga tcgacaatgc ctggtgccca tccggcccct gcgggccgtc
     8641 ggcagcggtc cagtcggcgt cactctggcg attcgttatc atggtgggcg cttataccac
     8701 gcgagcgtgc cggacaatcg ccagagcgcc gttcggccca tcgcatcccc atcttccggc
     8761 gcgcgacagg catgctgcgg tgcagtatgc tccggccacc cgcacgagga gacgcgctat
     8821 gctgaaactg aggcccaatt gcgagtgctg cgaccgcgac ctgccgccgg aatccgccga
     8881 agcggtcatc tgttcgttcg agtgcacctt ctgtcgcgac tgcgcctcga cccggctgcc
     8941 cggcggccgc tgcccgaact gcggcggcga actcgtgaag cgcccggtgc gacccgccga
     9001 tcgtctcgcg aagtttccgg cgtctagcga gcgagtgaag aaggcgcacg ccttttgtgc
     9061 ctgaggcgcc tcgccggtca ggcggccggg cccgcgttgg cgcgacactt gcgtcggcgt
     9121 ccccgatcat gacagctgcg gccggggcga cgccgccacg gcctggagac atgccaaaat
     9181 gacacgacga gcactcatca ccggtgtcac gggccaggac ggcgcctatc tcgcggagct
     9241 attgctcgcc aaaggttacg aggtgcacgg catcaagcgg cgctcctcat ccttcaacac
     9301 gcagcgcgtc gatcacctct atcaggaccc gcacgagggc gacgttccct tcctcatgca
     9361 ctatggtgac atgacggatt cgaccaatct catccgtctc gtgcagcaga tccggccgtc
     9421 cgaaatctac aacctcgcgg cccagagcca cgttgcggtt tccttcgaga gcccggaata
     9481 tacggccaat gccgatgccg tcggcgtgct ccgcctgctg gaagccatcc gcatcctcgg
     9541 catggagaag gattgccgct tctaccaggc atcgacatcg gaactctatg gcctcgtcca
     9601 ggaggtgccg cagcgcgaga cgacgccgtt ctacccgcgt tcgccctatg ccgtcgccaa
     9661 gctctacggc tactggatca cggtgaacta ccgcgaggcc tacgccatgc atgcctcgaa
     9721 cggcatcctc ttcaaccacg agagcccgat ccgcggcgag acgttcgtca cgcgcaagat
     9781 cacgcgcgcg gtcgcccgca tcgaggttgg cctcgaccag cagctctacc tcggcaacct
     9841 cgatgccaag cgcgactggg gccatgcccg cgactacgtc gaggggatgt ggcgcattct
     9901 ccagcaggag acgcccgacg actacgtgct cgcgaccggc gagacgaggt ctgtccgcga
     9961 gttcgtcgag atcgccttcg cccacgtcga caagcgtatc gtctggcggg gcaagggtgt
    10021 cgaggagagc ggggtcgacc agcggacagg caaggttctc gtcaagatcg acccgcgcta
    10081 cttccgcccg accgaggtcg atctgctgat cggcgatccg gcgaaagcgc gcgagaagct
    10141 cggctggacg ccgcaaacct cgtttgccgg cctggtgaag gaaatgatgg acggcgatct
    10201 ggcggccgcg cgacgcgagg catccaatgt ccgctgagat ctacagcctt gctggcaagc
    10261 gcgtgtgggt cgcggggcac cgcggcatgg tcggctcggc gatcgtgcgc cgnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn caacaatacg ctgcgtgccg agttcctcta cgacaacctc
    10501 gccatcgcga cgaacatcat acacgcctca caccgccagg gcgtcgagaa gctcatgttc
    10561 ctcggctcga cctgcatcta tccgcgcctc gcgccgcagc ccatgaacga agacgcgctg
    10621 ttgaccggtc cgctcgagcc gaccaacgaa ccatacgcta tagccaagat cgccggcatc
    10681 aagatgtgcg aggcctatcg cgcgcagtac ggctcggact tcatctccgt gatgccgacg
    10741 aacctttacg gaccgggcga caactaccat cccgagcaca gccacgtcgt ggcagcgctc
    10801 atccgccgtt tccacgaggc gaagctttcc ggtgctggcg aggtcgttgt ctggggcaca
    10861 ggcacgccgc gccgcgaatt cctctacgtc gacgacatgg cggacgcctc ggtctttgcc
    10921 atgaaacgct actcgggcgc cggcattctc aacatcggca ccggcgagga catcgcgatc
    10981 tctgagttcg cgcgcctcgt cgccaaggtg atcggctaca cgggccgcat cgcctacgat
    11041 acttcgcgac ccgatggcac gccgcgcaag ctcgtcgacg tcgcgcgcat gtctgcgctc
    11101 ggctggcgcg cctcgaccgg gctcgatgcc ggcctcgccc gcgcctacca aagtttcctc
    11161 gagagcctaa agtaggcagg ccgctccgcc tggagcggct ccacgctatc gcgtaggcgc
    11221 gatccagttg tggcccttga tgccgccgtc cacctcgacc gttgtccccg tcacataggc
    11281 cgagaagcga tcggacagca gtgccgtcgc ggttgcggcc atgtcggcgg cagcgccgag
    11341 gcgaccgagc gggatcatcc cgtcgaagga gaaggcggac gcattgaagc cgccgcccgg
    11401 caccgcgccc ggagcgagcg cattgacgcg gatgccgctc ggcccgagcg cacgggcaag
    11461 ctccttcacg accatggtca tgccggcctt cgatgccgag tagtgtggca ggttgcgcgg
    11521 tgtctcggcg tggagcgagg tgatcagcag caggcgcccc ttgatgccgg catcccgcat
    11581 ggcgaggccg atgccccgtg ccaggaagaa gcccgagcgc aggttcgtat tgatcatgcc
    11641 gtcccacacc tcctccgtca cggcgagcgt gtgatcggcc tcgcggcgcg ccggcgaggc
    11701 cgagtgcacc agcatcgaga cgacgggcgc gcgcgcaacc tcgaggagcg gcttccagcc
    11761 gtcgggcttg gagaggtcgc aggtgatctc gctggcactt ccgccggcag ccttgatctc
    11821 ggccccgacc tccgcattcc gcttcgggtc gacgtcggca aggatgacgt gcgcgccctc
    11881 gcgggcgagt gcggtcgcga tggcgcgacc gatgtttgag gccgagcccg tgacgagcgc
    11941 gcgctcgcca gggaagagct tggtcggcat gggagtaccc tcgcagtgca caggtggatt
    12001 gctgcggcaa tgctaggcgc ttgcggcgcc aactggaagg ggcgcagcgg cagctgatac
    12061 atgaagaggt cgatacaggt agtcgcgcac ccctcaccgc cgcgagacgt cggcgcgggc
    12121 ggtgctcgag gtggcggcga ggccggcggc cactgcggct tcgaggcgct cggcaccggc
    12181 gttctcggca agcgcttccg ggacgatcca catcacgtag tcggcgctcg cgatggcccg
    12241 ggcgcaggca agcgcgcccc gctccatgcg gcaggctgaa tccgatgtga tcgactgcgc
    12301 ctcgcggcgg tcgtggtgaa cgaggcgcat gtcaacgggt tggagcgtcg cctccgggcc
    12361 atccaggcgg acaccgcgga agacgcagcc gagctggtcg cggcaggcgc cggcccggcc
    12421 gccgaacgta ttgaggaagc cgaaagcctt gcggccgctg cgcagcgtcg cgggcgtatc
    12481 actgcctcgg ctgatgtagc agccatggtc ggcacacagg atgggatcgg ccgtcttgtt
    12541 gtgccgccgg atgccgcggg agcccggctg catcaccagg agcacggtga cggtcggctc
    12601 gctcggccaa ctcgcgaatg tcaggacacg cggcgaatcg gccggcggca tggcccacgg
    12661 tgccggcgag gctggagcgg gcagggtcgg cgcgggatgc gcgtgcgcgg gagccggacc
    12721 cgcgtcgtcg gcgata
//
LOCUS       DAORNH010000112        12622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3762_length_12622_cov_8.062226, whole genome shotgun sequence.
ACCESSION   DAORNH010000112 DAORNH010000000
VERSION     DAORNH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3762_length_12622_cov_8.062226"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>904)
                     /locus_tag="PK264_12520"
     CDS             complement(<1..>904)
                     /locus_tag="PK264_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD76741.1"
                     /translation="GQVLKIALPITLASISTPLVGIVDTAVVGQLGNEALIGGIAAAN
                     VIINLVFFTFNFLRSSTTAMVSQAFGAGHGIEMLAHALRAICLAFAAGLIAVALQWPI
                     AYAGFSLLGVGEDVRAAGMVYFGIRVWSAPLLLVNYALVGWLFGQGSFLPGLALQAVL
                     NLVNAILSIAFVLHFDWGVAGAALATVAAETATALAGGILIARALHGKARAVLSALPD
                     MRQLKRILSINADILLRSMIRFFSIALFTRESAAFGTAMLAANVVLMNLFYFSGAMIQ
                     GLAVAAQHLAGRAVGGCDRVMFETS"
     assembly_gap    905..928
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1300..1423
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1424..2411
                     /locus_tag="PK264_12525"
     CDS             <1424..2411
                     /locus_tag="PK264_12525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76742.1"
                     /translation="GLVWVQSKGDGMSEYARWTRRSADLWPEFAGELAGETGVSPHYY
                     KAGGVHTCLSEEELQARTNLIERMRQVHGPERYEARVLDRRELAEMLPGLGEAVIGGS
                     WSPHDGHASPLHLLRALHAGLLQGRGRYHADARVTAIARDGSGYRVTTAAGVFAAERV
                     VLAAGLANRALAPMVGLSAPVSPLKGQILVTERAATVLPMPTAFVRQTEEGTFLMGDS
                     YEDSGFDTASRPNVMAEIARRAARTFPFLRKLQVVRAWAALRVMSGDGFPIYEQSRTH
                     PGAFVVNCHSGVTLAGAHALALAPMIARGRLDEEFAAFSAGRFDVQAHRDVA"
     gene            2383..2682
                     /locus_tag="PK264_12530"
     CDS             2383..2682
                     /locus_tag="PK264_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD76743.1"
                     /translation="MFKRIATSRKARLSFDFEGHRLEAEDGDTVAAALLLAGERVLRS
                     TAVSASERGPFCLMGVCFECLVEIDGIPNRQACMVKVLPGMRVRRQRGAARVGST"
     gene            2679..4064
                     /locus_tag="PK264_12535"
     CDS             2679..4064
                     /locus_tag="PK264_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76744.1"
                     /translation="MSAYDVAIIGAGPAGMAAAVEASRAGFSVVVFDEQDAPGGQIYR
                     AIERADAGRLALMGEDYARGRALAEAFRTSSAVYMPGTIVWNVDASLRINYSGATGSG
                     EVTAGSVIIATGAMERATPLPGWTLPGVMTAGALQILLKAHELVSDDTVLVGCGPLLF
                     LVAQQMTEAGSPPRAVVETTAPGALRRALPHLFGALRAPAYLRKGLAMIRQLARAGVP
                     VYRHAQAVAIEGREAAEAVSFTIKGQRHRISAGTVGLHQGVIPNQQITRLLGCEQDWS
                     ASQRCFVPRLDPFGETRIQNVYVAGDGAGIGGAVSAALGGRLAALRIAARAGKATTGE
                     ARSVMARLARDRAIRPFLEALYAPSDEVLVPADDTLVCRCEEVTAGQIREAVALGAPG
                     PNQAKAFLRCGMGPCQGRICGPVVTEVIAAARRTPQDAVGYYRIRPPLKPLSVAEIAG
                     LAPAAEGQPLD"
     gene            4124..4474
                     /locus_tag="PK264_12540"
     CDS             4124..4474
                     /locus_tag="PK264_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003592696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD76745.1"
                     /translation="MIKRHMQTRINHRVVEHNRVLYFGGLIADDLSVDMKGQTQQICR
                     KIDELLQKVGSSKAHLLTAMIYISDFSQKEGMNEAWLEWLPGELLPTRATIGVADLGE
                     NVLIEVVVSAAKTA"
     gene            4754..6445
                     /locus_tag="PK264_12545"
     CDS             4754..6445
                     /locus_tag="PK264_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HRD76746.1"
                     /translation="MNIRLHGLTTILSALVVLTAPTAVLAQDNLVTGELITTVNSGTP
                     GKGGEVTFTVTRDITNWNVTSALGNNAVVRTVTLPIVPSAFMVQPDFTLKMNTDLLES
                     AELTSTDPQTVVYRIREDAVWSDGVPITGDDFIYFWKTQNRRDCPECLINASYGHDFI
                     ETLEQDETGKVVTATFSEPFVGWQGLFMFLYPAHLAEKHGDIAESYNNFLSNEVPAWS
                     GGPYMVESFDPGQLVTLVPNPKWYGEKGPYLDKLKFRIITDSTQQLTALENGEVDVIY
                     PQGATQDMVEQAAGLDYLGIDFQMNPAANWYFMGLNSKAGPMSDIALRKAVLTAIDAG
                     DLKAKTADPYLRNWPHMGSVMFLPNEAGYADRRGARGYGTGDVEKAKGILSEAGYKLS
                     GGSLLDPSGKPVSTLRLGFPPGYPAANDMARLITGYIAPLGLKTDLLTGPNAHADYLL
                     SGNFDLYLNYFSQQVFPAVKAGQIFLRDALQNYFGFNDPKIEEIIGKALAASSIEESA
                     AILSEADELAMDYAALFPIYQLPTALIYKEAILNLRDNPNQLGPAYNTAEWGLAE"
     gene            6537..7517
                     /locus_tag="PK264_12550"
     CDS             6537..7517
                     /locus_tag="PK264_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76747.1"
                     /translation="MLAYLVRRLLVMIPMMLIASFLMFVVISFSGDPLAELKTMQPPP
                     PPEVLHAMAVKLRLDQPLLDRYWMWLSGLFVGDFGPSIHNIDIGAELVKRLGVSLRLL
                     ALGIVLAFLMAVAASVIGAVLQFSWADHIISFVAVAMISMPVFWLALQLKRLAISINQ
                     ELGFRLLYTSGDGAGMTASTPWDRVLLLIGVLALPTIALSMQAFGTWSRYGRVALIDA
                     LNSEYVKLARAKGLSETRVVLAHGLRTSLAPMLTVVAAGGAGILGESVVTETVFQWRG
                     MGDLLVTGIQNNDVYVVLGWLLVAGLIIMVFNLIADILYAVLDPRIRYER"
     gene            7522..8406
                     /locus_tag="PK264_12555"
     CDS             7522..8406
                     /locus_tag="PK264_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76748.1"
                     /translation="MEIGQAPAQEQVYRPWRAIARKFFSYKASVVSLVILILIVLFAF
                     AGGPLWKYSYAVSTSDLSAPPSWEHPFGTDHLGYDLMAGVMRGTQRSLSIAGTVAVLA
                     TLIGSIWGAVAGYVGGWADSLLMRIVDLIMIFPLLAVAAFLARQSQGGEHGWLLVALV
                     LATLSWTVLARVVRSVVLSLRTREFVIAAKIAGAGPVRIIFRHLLPNTVGPIIVAATV
                     LVASAILSETALSYLGFGVQPPDTSLGLLINQAQGAISTRPWLFYFPGLFIILIALCV
                     AFIGDGLRAALDPKQQRK"
     gene            8415..10235
                     /locus_tag="PK264_12560"
     CDS             8415..10235
                     /locus_tag="PK264_12560"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76749.1"
                     /translation="MSESVHIAGGTGAPLVSVSGLTVSFDRHREEPIEAVRDVSWSIG
                     SGEVLALVGESGSGKSVSALAVMGLLPRNARVAGSIRWRGEELLGASEKRRRALRGSR
                     IGLVPQDPMTSLNPVYTIGAQIREGIRAHQKLSEREMAERTLELLETMGIPHARERMN
                     SYPHELSGGMRQRVVIAMAMANNPDLIIADEATTALDVTVQAQVLDALKKAQALTGAA
                     LLLITHDLGVVAGRADRVAVMQRGEVVEQGTVDEIFYQPASSYTRKLLHSIPRLNHTP
                     DLSAPSDVAPDAEVIHSAFERKVGAEGAPILSMRNVGKHFPVYSRGVIRGRVGVIKAV
                     AGVDLDVEAGTTLGIVGESGSGKTTLIRSLFNLEPITHGTILFDGQDVHRMPPRDRRR
                     MRKCVQMVFQDPYASLDPMMTVRDILMEPAVINRMDRKLAERRVMELLERVHLKPEHA
                     ARYPNEFSGGQRQRIAIARALMLYPRLLVLDEPVSSLDVSIQAEVLTLLKELQKDLNL
                     SYLFVSHDLSVVAEIAHSVIVMYRGRIVERGRVDELFRNPKHPYTRALLSAVPIPDPR
                     TERRRERIVFNSDTLASPITPVERTGMWRRLFGGRTQEAS"
     gene            10235..11527
                     /gene="emoA"
                     /locus_tag="PK264_12565"
     CDS             10235..11527
                     /gene="emoA"
                     /locus_tag="PK264_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH-dependent EDTA monooxygenase EmoA"
                     /protein_id="HRD76750.1"
                     /translation="MRKRRMYLVSWLNSSGVLPNSWNEGRGNRARIFDLENYIRSAEI
                     ARRGRIDAFFLADQPQLTPNPKVRPEYPFDPIVLAAAITGRVPDIGGIVTASTSFSLP
                     YTLARQIASVNLLSGGRIGWNAVTTANPAVAANYGAAIATHDNRYERAEEFLEVVHGL
                     WNSWKFPWDEAIGPNPNPFGEVMPINHEGKYFKVAGPLNVPLPPYGPPVVVQAGGSDQ
                     GKRLASRFGEIIYAFLGSKPAGRRFVAEARAAARAQGRPEGSTLVLPSFVPLIGSTEA
                     EVKRLVAEYEAGLDPAEQRIEALSKQLGIDLERINVDQVLQEKDFNLPKESATPIGIL
                     KSMVDVALDEKLSLRQLALRMRLIAGTPDQVADRLIDWWQDEAADGFVINAPLLPDAL
                     EIFVDQVVPILQSRGVFPRSYTESTLRERLGLPRNPLG"
     gene            11640..12233
                     /gene="emoB"
                     /locus_tag="PK264_12570"
     CDS             11640..12233
                     /gene="emoB"
                     /locus_tag="PK264_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN oxidoreductase EmoB"
                     /protein_id="HRD76751.1"
                     /translation="MTYSIVAISGSPSRNSTTAKLAEYALAHVLARSDSQGRHIHVID
                     LDPKALLRGDLSNAKLKEAVDATCNADGLIVATPIYKASYTGLLKAFLDILPQFALAG
                     KAALPLATGGSPAHVLALDYGLRPVLHSMGVRHVVQSFFLVQSQFSVVDGKLAVEDDV
                     ASQLNNAIDHFRLSLSSEPSTRHLGHPRPSLDATRAA"
     gene            complement(12264..12539)
                     /locus_tag="PK264_12575"
                     /pseudo
     CDS             complement(12264..12539)
                     /locus_tag="PK264_12575"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
BASE COUNT         2304 a         3828 c         3930 g         2412 t
ORIGIN      
        1 ccgatgtttc gaacatgacg cggtcacacc cgcccaccgc ccttccggca aggtgctggg
       61 cggcgacggc aagcccctgg atcatcgcgc cggaaaaata gaagaggttc atcagcacga
      121 cgttggctgc gagcatcgcc gtgccaaaag cggcactctc ccgcgtgaag agagcgatgc
      181 tgaaaaagcg aatcatggat cgcagaagaa tgtctgcatt gatgctgagt atccgcttca
      241 gttggcgcat gtccggcagg gctgaaagca ctgcccgtgc ctttccatga agagcgcgcg
      301 ctatcagaat gccgccggcg agagcggtgg ccgtctctgc ggccaccgtc gccagcgccg
      361 cgccggcaac accccaatcg aaatggagga cgaaagcgat actgagtatt gcgttgacga
      421 ggttgagcac tgcctggagg gcaaggcccg gcaggaaaga gccctggccg aagagccagc
      481 ccaccagcgc ataattgacg agcagcagcg gcgctgacca gacgcggatg ccgaaataga
      541 ccattccggc tgcccttaca tcctcgccga cgccgagcag ggaaaagccg gcataggcaa
      601 tcggccactg tagcgccact gcgatgaggc ctgccgcgaa agccaagcat atggcgcgga
      661 gcgcatgcgc gagcatctct atcccgtgtc ccgccccgaa cgcttgcgaa accatggcag
      721 tggtggagga acgcaggaag ttgaaggtga agaagacgag gttgatgatc acgttggcgg
      781 cagcgatgcc gccgatcagg gcttcgttcc ccaactgccc gacaactgca gtgtcaacga
      841 tgccgaccag gggtgtcgat attgatgcca gggtgattgg caacgcaatc ttcagcacct
      901 ggccnnnnnn nnnnnnnnnn nnnnnnnngt aacggtggtg gcaggcatct gcgaaccagg
      961 ggaagattcc tggtcaagcc gtggctggtc aattgtcatc gggcggcgct cccgcttctt
     1021 ttgaacgggc gcaccctgcc gcacccagcg cagcaggatg cggtcatacg atcagtcgca
     1081 ctcgctgccg ttctgcccat tcactcagga ttggaccact ttgcacggtg tgtcgaaggc
     1141 ggcgcgatct gctcccgcat cgcgggaatc cttgcagcgc gctatcgact gcgctgttga
     1201 ttgagtttag ccggtttctg acatgcgccg tgccaaccgg ccggtgccag cggaggtggc
     1261 ttcaccatgg cggttcagca tgccgatacg atcgtcgtcn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgggctc gtgtgggtgc
     1441 agtcgaaagg cgacggcatg tccgaatatg cgcgttggac acgccgctcg gccgacctct
     1501 ggccggaatt cgccggagag ctcgccgggg agaccggcgt atcgccgcat tattacaagg
     1561 ctggaggagt gcatacctgc ctttctgagg aggaactgca ggcccgtacc aatctcatcg
     1621 aacggatgcg gcaagtgcat gggccggagc gctatgaagc aagggtcctc gaccgccgcg
     1681 agcttgccga gatgcttccc ggcttgggtg aagcagtcat tggcggctcg tggtccccgc
     1741 acgacggcca tgcgagcccg cttcacctct tgcgagcgct gcatgctggg ctcctgcaag
     1801 ggcgcggacg ctatcatgcc gacgcccgtg tgaccgcgat agcaagggac ggctcgggtt
     1861 atcgcgtcac gaccgccgcc ggggtgttcg cggcggagag agtggtgctc gccgctggcc
     1921 ttgctaaccg cgcgctcgcg ccaatggtgg ggctcagcgc gccggtctct ccgctcaagg
     1981 ggcagattct tgtcacggag cgcgcagcga cggtcctgcc tatgccaacc gccttcgttc
     2041 gccagacgga ggaaggcaca ttcctcatgg gagacagcta cgaagactcg ggcttcgata
     2101 ccgcgtcgcg gccaaacgtc atggcggaga ttgccagacg cgccgcccgc acctttccat
     2161 ttctcagaaa gctgcaggtc gtgcgggcct gggccgcgct ccgcgtcatg tccggcgacg
     2221 ggtttcccat ctacgagcaa tcgcggactc atccgggcgc cttcgtggtg aactgccatt
     2281 ccggcgtcac tctggcaggc gctcatgcgc tggcgctcgc gccgatgatc gcgcgcggcc
     2341 gccttgatga agaattcgcc gccttcagcg cagggaggtt tgatgttcaa gcgcatcgcg
     2401 acgtcgcgta aggcaaggct ctctttcgat ttcgaagggc acagactcga ggcggaggac
     2461 ggcgataccg tggcggccgc gctgctgctt gccggcgaaa gggtcctgcg cagcacagcg
     2521 gtttcagcgt ccgaacgcgg gcccttctgc ctcatgggcg tgtgcttcga atgcctggtc
     2581 gagatcgacg ggatccctaa ccggcaggcc tgcatggtga aggtgctgcc aggtatgcgg
     2641 gtgcgccgcc agcgcggcgc ggcgcgggtg ggaagcacat gagcgcatat gatgtcgcga
     2701 taatcggcgc cggccccgcg ggtatggccg cggcggtgga ggcatctcgc gccggctttt
     2761 ccgtagtcgt cttcgatgag caggacgcgc ctggcgggca gatctatcgc gccatcgagc
     2821 gggcggatgc ggggcggctg gcgctgatgg gtgaagatta tgccaggggt agagctcttg
     2881 ccgaggcttt caggacgagt tccgcggtct acatgccagg cacgattgtc tggaatgtcg
     2941 atgcgtcgct ccgtatcaac tacagcggcg cgactggcag tggcgaagtc actgcgggat
     3001 cggtgatcat agccacgggt gccatggagc gcgccacgcc tttgccgggc tggactctgc
     3061 cgggcgtgat gacggccggc gcgttgcaga tcctgcttaa ggcgcacgag ctcgtcagtg
     3121 acgatacggt attggtcggc tgtggccctc tccttttcct ggtggcacag caaatgacgg
     3181 aggctggatc gcctcccaga gcggtcgtcg agaccacagc gcctggcgcg ctccgccggg
     3241 cgctgccgca tctcttcggg gcgttgcgcg ctccggccta tctgaggaaa ggcttggcca
     3301 tgatccggca gctcgcccgc gcgggcgtgc cggtctatcg ccatgcgcag gcggttgcga
     3361 tcgagggccg cgaggcggcg gaagccgtca gcttcacgat caaaggccag cgccatcgca
     3421 tttcggcggg caccgtgggc ctgcaccagg gcgtgatacc caaccagcag atcacgcgcc
     3481 tcctcggttg cgagcaggat tggagcgcaa gtcagcgctg tttcgtgcct aggctggatc
     3541 cgttcggcga aacgagaatc cagaatgtct acgtcgccgg cgacggagca ggaatcggcg
     3601 gcgccgtttc cgccgctctc ggagggaggc tcgcggccct gcgcatcgcc gccagggcgg
     3661 gcaaggctac tacgggcgaa gcccggtccg taatggcgcg gctcgcgcgc gatcgggcga
     3721 tccgcccctt cctggaggcg ctctatgcgc cgtccgatga agtgcttgtg cccgcggatg
     3781 atacgctcgt gtgccgctgt gaagaagtaa ccgccggaca gatccgcgaa gccgtggcgc
     3841 ttggggcgcc cgggccaaat caggcgaagg ccttcctgcg gtgcggcatg gggccgtgcc
     3901 aaggccgcat ctgcggcccc gtggtcacgg aagtcatcgc agcggcgcgc cggaccccgc
     3961 aggatgctgt tgggtactac cgcatccgcc ccccgcttaa gccactctcc gtagccgaga
     4021 tagcgggcct ggcgccggct gcggagggcc agcccctgga ctagggccgt gctgctgaat
     4081 ctgcaatttc catcaacacc agcgaacaat gaaggagaag gttatgatca agcgtcacat
     4141 gcagacacgc atcaaccatc gggtggtcga gcacaacagg gtgctctatt ttggcggcct
     4201 tattgccgat gacctgagcg tcgacatgaa gggccagaca cagcagatct gccgcaagat
     4261 cgacgaactg ctgcaaaagg tgggctcctc aaaggcgcat ctgctcacgg cgatgatcta
     4321 catctccgat ttctcgcaga aggaaggaat gaacgaggcc tggctcgaat ggctgcccgg
     4381 tgagcttttg cccacccgtg cgaccatcgg cgtcgctgac ctcggcgaaa acgtcctgat
     4441 cgaggtggtc gtgtccgcag ccaagactgc gtaggaatcc gggtccgccg gtcaatggct
     4501 gcctggtcct acgatgcggg aatccagccg tggctccatt gacagggcgg tcatctcacg
     4561 aaactctgct tgccgtgctg caaaacaagc aacagtaaaa acgcagcgaa gaacgtattg
     4621 tgttgaaaaa caatacgggg aacactcgca gtcgtgatcc ggtattgaga agtcgtacaa
     4681 caaaaaatct caattcgggt ccgatttgca tcgggttaat agaaatatgt actcgtatgg
     4741 atgggagtaa tacatgaaca tccgactaca tggtctaaca acaattctat ctgcattggt
     4801 agtgttgacg gcgccgaccg ctgtccttgc tcaggacaat ttggtcaccg gggagttgat
     4861 cacgaccgtc aattcgggca cgcccggaaa gggcggtgag gttaccttca cagtcacgcg
     4921 cgacatcacc aactggaacg tcacctcggc cctcggcaac aacgccgtgg tccgcacggt
     4981 aacgctgcca atcgttccgt cggctttcat ggtgcagccg gacttcaccc tgaagatgaa
     5041 caccgacctc ctggagagcg ccgagcttac atcgaccgat ccccaaaccg tggtctacag
     5101 gatcagggaa gatgcggtgt ggagcgacgg cgtcccgata acgggcgacg acttcatcta
     5161 tttctggaag acgcaaaacc gccgcgactg cccggaatgc ctgatcaatg cgagctatgg
     5221 ccacgacttc atcgaaacgc tcgagcagga cgagaccgga aaagtcgtga cggcgacatt
     5281 cagcgagccg ttcgtgggct ggcaaggact tttcatgttc ctctatccgg ctcatctagc
     5341 cgagaagcac ggcgacatcg cggagagcta caacaacttc ctctccaatg aggtgccggc
     5401 ctggtcgggc ggaccctaca tggtcgagtc gttcgatccg gggcaactcg tcacgcttgt
     5461 gccaaatccg aaatggtatg gcgaaaaagg gccttatctg gacaagctga aattccggat
     5521 catcaccgat tcaactcagc agctgacggc gctcgaaaac ggcgaggtcg atgtgatcta
     5581 tccgcagggc gccacccagg acatggtcga gcaggcggcg ggcctcgatt atctcggcat
     5641 cgatttccag atgaatccgg ccgccaactg gtatttcatg ggcctcaact ccaaagccgg
     5701 cccgatgtcc gacatcgcgc tgcgcaaggc ggttctgacg gctattgacg ccggtgacct
     5761 gaaggccaag accgctgatc cgtatttgcg caactggccc catatgggca gcgtgatgtt
     5821 cctgcccaac gaggcaggtt atgccgaccg caggggagcg cgcggctacg gtacggggga
     5881 cgtggagaag gcgaagggga tcctctccga ggcgggctac aagctcagcg gaggcagtct
     5941 gctggatccc tccggcaagc ctgtatccac gcttcggctt ggctttccgc ccggctatcc
     6001 ggcggccaac gacatggcac gactgatcac aggctatatc gccccgctcg gcctcaagac
     6061 ggatttgttg accggcccga acgcccacgc cgactatctc ctgagcggca atttcgacct
     6121 ttacctcaac tatttctcgc agcaggtctt tcccgcggtg aaggcggggc agattttcct
     6181 cagggatgcc cttcagaact atttcggctt caatgatccg aaaatcgagg agatcatcgg
     6241 caaggctttg gcagcctcgt caatagagga atctgccgca atcctgtccg aggccgacga
     6301 gctcgcgatg gattacgcgg cgctattccc gatctatcag ttgccgacgg cgctgatcta
     6361 caaggaagcg atcctcaacc tgcgcgacaa tccgaaccaa cttggtccgg cctacaacac
     6421 ggccgaatgg ggtttagcgg agtgaatggg cggaaggggc cgggcagctt ccggcatgac
     6481 ggccccttca cccaaatcgt tccgccgatg attttccaag ctgcagggag ttgagcttgc
     6541 tagcctatct ggtcaggcga ctgcttgtca tgatcccgat gatgctgatc gccagctttc
     6601 tcatgttcgt cgtcatcagc ttctccggcg atccgctcgc ggaattgaag accatgcagc
     6661 cgcctccgcc gcccgaggtg ctgcacgcca tggcggtcaa gttgaggctc gatcagccac
     6721 ttctcgaccg atactggatg tggctctccg gcctgttcgt cggggatttc gggccgagca
     6781 ttcacaatat cgatatcggt gcggagctcg tgaagcgcct tggtgtttcg ctgcggctcc
     6841 tggcgctcgg catcgtgctc gcattcttga tggctgtcgc ggcgtcggtt atcggcgcag
     6901 tgctgcagtt ttcctgggcc gaccatatta tcagcttcgt cgccgtcgcg atgatatcga
     6961 tgccggtgtt ctggcttgcg ctgcagctca agcggctggc gatcagcatc aatcaggagc
     7021 ttgggttcag gctgctctat acgtccgggg atggcgcggg catgacggcg agcacaccgt
     7081 gggatcgggt gctgcttctc atcggtgtgc tggcgctccc cacgatcgcg ctttccatgc
     7141 aggctttcgg gacatggagc cgctacggcc gtgtggccct aatcgacgcg ttaaacagcg
     7201 aatatgtgaa gcttgccagg gccaagggcc tttcggagac gcgcgtggtg ctggcgcacg
     7261 gcctgcgaac ctcgcttgcg cctatgctta ccgttgtggc tgcgggcggc gccggcattc
     7321 tcggcgagtc cgtcgtcacc gagacggtct tccaatggcg cgggatggga gacctcctcg
     7381 tcaccggaat ccagaacaac gacgtctatg tcgttctcgg ctggctgctg gtggccggtc
     7441 tcatcatcat ggtcttcaat ctgattgcgg atatcctcta tgccgtcctc gacccccgca
     7501 tccgctatga gcgatagagc agtggaaata ggacaggcgc ctgcgcagga gcaggtctac
     7561 cgtccctggc gtgccattgc gcgcaagttc tttagctaca aggcatcagt cgtctcgctc
     7621 gtcatcctga tcctgatcgt gctcttcgcc ttcgccggcg ggcccttgtg gaaatattcc
     7681 tatgccgtat cgacgtccga cctgtcggcg ccgccgagct gggagcatcc cttcgggacc
     7741 gatcatctgg gctacgatct catggcgggc gtgatgcggg gcacgcagcg ctcgctctcc
     7801 atcgctggaa cggtggcggt gctcgccacg ctgatcggct cgatctgggg tgcggtcgcc
     7861 ggctatgtcg gcggctgggc ggactcgctc ctgatgcgga tcgtcgatct catcatgatt
     7921 ttcccactcc tggcggtcgc cgccttcctc gcccggcagt ctcagggcgg ggagcacggc
     7981 tggctgctgg tggcgctcgt gctggcgacc ctctcatgga cggtgctggc gcgtgtcgtg
     8041 cgctcggtag tgctctcgct tagaacgcgc gaattcgtca tcgcggcgaa gatcgccgga
     8101 gcggggccgg tgcgcatcat cttccgccac ctgcttccga acacggtggg gccgatcatc
     8161 gtcgcggcca cggtgctggt ggcgagcgcc atattgtcgg agaccgctct ttcctacctc
     8221 ggcttcggcg tgcagcctcc ggacacctcg ctcgggctcc tgatcaacca ggcccaaggt
     8281 gcgatttcta cccgcccctg gctcttctac ttcccggggc tcttcatcat cctgatcgcg
     8341 ctctgcgtgg ccttcatcgg agacggcttg cgggccgccc tcgatcccaa gcagcagagg
     8401 aaatgaggcc ccatatgagc gagagcgtcc atatcgcggg cggcaccggc gcgccgctgg
     8461 tcagcgtctc cggccttacc gtcagcttcg accggcacag ggaagagccg atcgaggcag
     8521 tgcgcgacgt aagctggtct atcggatcgg gcgaggtgct ggcgctggtc ggcgaatccg
     8581 gctcgggcaa gtcggtctcg gcgcttgccg tcatgggcct ccttcccagg aacgcgcgcg
     8641 tggccggctc catacgctgg cgcggggagg aactgctcgg tgcctccgag aagcggcgcc
     8701 gggccctgcg cggcagccgc atcggcctcg tgccgcagga tccgatgacg tcgctcaacc
     8761 ccgtctacac catcggcgca cagatcaggg aaggcattag ggcccaccag aagctgagcg
     8821 agagggagat ggccgagcgc acgctggagc ttctcgaaac gatgggcatc ccgcatgcgc
     8881 gcgagcgtat gaacagctat ccgcacgagc tttcaggcgg catgcgccag cgcgtagtca
     8941 tcgccatggc gatggcgaac aaccccgatt tgatcattgc cgacgaagcg accaccgcgc
     9001 tcgacgtgac cgtgcaggcc caggtgctgg atgcgctcaa gaaagcacag gcgctcaccg
     9061 gggcggcgct gcttctcata acgcacgacc ttggtgtggt ggcgggccgt gcagatcgcg
     9121 tcgcggtcat gcagcgcggc gaagtggtcg agcaaggcac ggtggatgag attttctacc
     9181 agccagcgtc atcttataca cgcaagctgc tccacagcat cccccgattg aaccacacgc
     9241 ctgacctgag tgcccccagc gatgtcgcgc ccgatgccga ggtgatccat tcagcctttg
     9301 aaaggaaggt cggtgctgag ggagctccga tcctatcgat gcggaatgtc gggaagcatt
     9361 ttcccgtcta ttcgcgcggc gttattcggg gcagggtggg ggtgatcaag gccgtcgccg
     9421 gcgtcgatct cgacgtggag gcgggcacca cgctcggcat cgtcggcgag tcgggttcgg
     9481 gaaagacgac gctcatccga tcgcttttca acctggagcc aatcacccac ggcactattc
     9541 tgttcgatgg ccaggacgtt caccgaatgc cgccgcgcga taggcggcgg atgcgcaaat
     9601 gcgtgcagat ggtcttccag gatccatatg cgtcgctaga cccgatgatg acggtcaggg
     9661 acatcctgat ggagccggcg gtcatcaacc gaatggatcg caagctcgct gagcgccgcg
     9721 tcatggagct gctcgagcgg gtgcacctca agccggagca cgccgcgcgc tatcccaacg
     9781 aattctccgg cgggcagcgc cagcgcatcg ccatcgcgcg tgcgctgatg ctgtatccgc
     9841 ggctcctggt gctcgacgag cccgtctcct ctctcgacgt gtctattcag gcagaggtgc
     9901 tgacgcttct caaagaactg cagaaggacc tcaacctttc ctacctcttc gtctcgcacg
     9961 atctttcggt ggtcgctgag atcgcccatt ccgtgatcgt gatgtatcgc ggccgcatcg
    10021 tcgagcgcgg ccgggtcgac gagctcttcc gcaatccgaa gcacccctac acacgcgcgc
    10081 tgctttcggc ggttccgatc ccagacccga ggacggagcg tcggcgcgag cggatcgtct
    10141 tcaatagcga cacccttgcc agcccgatca cgccggtcga acggaccgga atgtggcggc
    10201 gtctttttgg cggcagaacc caggaggcct cttaatgcgc aaacgcagga tgtatcttgt
    10261 cagctggctg aactcctcgg gggttctccc caattcctgg aatgagggcc ggggaaaccg
    10321 ggcgaggatc tttgacctgg agaattatat ccgcagcgcc gagatcgccc ggcgcggccg
    10381 gatcgatgcc ttctttctag ccgaccagcc gcaactaacc cccaacccga aggttcgtcc
    10441 tgagtatccg ttcgacccta tcgtgcttgc ggcagcgatc acaggccgcg tgccagacat
    10501 cggcgggatt gtgactgcct ccaccagctt cagcctgccc tacacgctcg ccaggcaaat
    10561 cgcctcggtg aacctgcttt cgggcggccg catcggctgg aacgcggtca caaccgccaa
    10621 ccccgccgtc gcggccaact acggcgccgc gattgccaca catgacaatc gctatgagcg
    10681 ggcggaagag ttcctggagg ttgtccatgg cctttggaac agctggaaat tcccgtggga
    10741 tgaggcgatc ggccccaatc caaatccgtt cggcgaagtg atgccgatca accatgaggg
    10801 gaaatatttc aaggttgcgg gtcccctcaa cgtgcctttg ccgccttacg gcccgccggt
    10861 ggtcgtgcag gcaggcggct cggaccaggg caaaaggctc gccagccgtt tcggcgagat
    10921 catctatgcg ttcctgggga gcaagccggc cggccggcgc ttcgtcgccg aggcgcgagc
    10981 ggccgctcgc gcgcagggcc gccccgaggg ctccacgctg gtcctgccga gctttgtgcc
    11041 gctgatcggg tcgaccgagg cagaggtcaa gcggctcgtc gccgagtacg aagccgggct
    11101 cgatccggcc gagcagcgga tcgaggcgct gtcgaagcaa ttgggcattg atcttgaaag
    11161 gatcaacgtt gaccaggtgc tgcaggagaa ggacttcaat cttccgaagg agtccgccac
    11221 gccgatcggc atcctgaaat ccatggtcga cgtcgctctc gacgagaagc tttcactgcg
    11281 gcaactggct ttgcgcatgc ggctgattgc gggcacgccg gatcaggtcg ccgaccgtct
    11341 catcgattgg tggcaagacg aggcggcgga tggatttgtg atcaatgccc cgctgctacc
    11401 cgacgctctg gagatttttg tcgatcaggt cgtaccgatc ctgcagtcgc gaggcgtttt
    11461 tccccgcagc tacaccgagt cgaccttgcg tgagaggttg ggtttgcctc gaaatccgct
    11521 aggttgacca agcgaaaatc atggtttgtt ccacatctgc atacatttac aaatacaaaa
    11581 ttgtatgctt tatttgggct gtgagtggat gccacccatg cgtttaagag gaagcggaaa
    11641 tgacctactc catcgtggct atctccggca gtccatcgcg caactcaacc actgctaagc
    11701 tggcagagta tgctctggca cacgttttgg cgcgaagcga ctcgcaaggc aggcatattc
    11761 acgtcatcga cctcgatccg aaggcgttgc tccgcggcga cttgagcaat gccaagctca
    11821 aggaagcagt ggatgccacc tgcaacgcgg acgggctcat cgtggcgacg ccgatctaca
    11881 aggcttctta tacgggtctg ctgaaggctt ttctcgacat cctcccgcag ttcgctcttg
    11941 ccgggaaggc tgcgctgccg cttgccacag gcggcagccc tgcgcatgtg ctcgccctag
    12001 attacggatt gcggcccgtc ctgcactcga tgggcgtacg ccatgtggtg cagagctttt
    12061 tcctcgtgca gtcccagttt tccgtcgtcg atggaaaact ggccgttgag gacgacgtgg
    12121 cgtcgcagct caacaacgcc atcgatcatt tccgtctttc gctgtcgtcg gagccttcga
    12181 cgcggcatct gggtcaccca cgcccctcgc tcgatgcgac gcgcgcggcc tgacatcact
    12241 tgaactgcgc caaatcttgc gcgttagaat tcggctccaa catagatgtc ggcgatgccg
    12301 gctcgaataa cctcgacaat ctcgtcatag cggcggttga tcatggtggc gatacgcttt
    12361 gcacaggcgt ccgcatcgcg ccgcatcagc atatcgatga attcgccgtt gtgacggaca
    12421 ccgggaggag gggggcgcag gcgtaagtcg atccagcgca caaaatgaat gcgcccattg
    12481 atctgctcga gtgccttcac caactctgcg ttgcccgtca ttctcgcaat gcgcaagtga
    12541 aactcctcgt catcgcgggg gttctgtcaa gggtcctcga ccgccgcgag cttgccgaga
    12601 tgcttcccgg cttgggtgaa gc
//
LOCUS       DAORNH010000113        12529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3799_length_12529_cov_2.395463, whole genome shotgun sequence.
ACCESSION   DAORNH010000113 DAORNH010000000
VERSION     DAORNH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_12529_cov_2.395463"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PK264_12580"
     CDS             complement(<1..432)
                     /locus_tag="PK264_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD76752.1"
                     /translation="MLVAAFAPVAAESVAAAGHAPPAQNRTTPRLASQAKSDTLAERF
                     ETAPIRTLGAKEHLFCEGDPASHVYRVEAGHVVLYRTMPDGRRQVIDFAYPGDVIGLG
                     ALATHGHNAQATGTAVLRCLPVALLQALAGCDPRLGLKLYEA"
     gene            599..1387
                     /locus_tag="PK264_12585"
     CDS             599..1387
                     /locus_tag="PK264_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD76753.1"
                     /translation="MPDRQPKHILVVDDEAPVRTLLRDCFETEGYVVAEAADAAAAKA
                     HISAAPPSLVTLDLKLGGDDGLVLAREIRAICNVPIVMISGKGDTIDRVVGLELGADD
                     YISKPFHVREVLARVKAVLRRYETGTTALPGQLAPPPAAAGTTLTSSPVIGGAADERY
                     ELAGLVLDIPRRELRDSDGQIIELTTAEFNMLAMFAPRPHRVLSRDNIMDLLKGHEWS
                     PFDRSIDSLVVRLRRKIEPSPDTPRIIKTVRGVGYVLASDVKRL"
     gene            complement(1391..1735)
                     /locus_tag="PK264_12590"
     CDS             complement(1391..1735)
                     /locus_tag="PK264_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76754.1"
                     /translation="MTGQRQARNARQLQLRSIGFAALVAALNLTASTAAAQSAGDAAY
                     ERRCSKCHGALAEFAKSDLEVRDGVLVGRQSHTAVAEYLKNHGRARASEISALVERLR
                     QVLEAGAGVPKT"
     gene            2537..>3294
                     /locus_tag="PK264_12595"
     CDS             2537..>3294
                     /locus_tag="PK264_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRD76755.1"
                     /translation="MHWVASAGESDLRRLLVAYVILGLVAGLAAYGLGQPRVAHALWI
                     VATLPVVTTLGASIVRDFAAGRAGVDAIAFVSMTTALVLGEPLAATVVAVMYSGGMLL
                     EDLAVARANRDLRLLIDRAPRIAHLLAGGTVRDVGIDAVVIGDTLLVRSGEVIPVDGK
                     LLADRALIDEAALTGEALPLARLRGDPLRSGTLNAGPTFEMRATAAADSSTYAGIIRL
                     VTAAQTAKAPFMRLADRFALILLPDNSMKLLTPAL"
     assembly_gap    3295..3336
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3337..>3630
                     /locus_tag="PK264_12600"
     CDS             <3337..>3630
                     /locus_tag="PK264_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD76756.1"
                     /translation="PCPLILAAPIAFISGISRAARRGILVKGSAAMERLAGATTLLFD
                     KTGTLTIGGARLAAVETAPGVTGDEALALAASLELGSPNVVAASIVAAARERGL"
     assembly_gap    3631..3752
                     /estimated_length=122
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3753..>4108
                     /locus_tag="PK264_12605"
     CDS             <3753..>4108
                     /locus_tag="PK264_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRD76757.1"
                     /translation="WAKRALQRATWRNALTVFIAVDGRPAAALLLADELRRDAPRAIR
                     ALREAGISRLLMVTGDRPDAAEAVGTALGLDAVLAERTPSEKVEAVASEQSRARTIMV
                     GDGINDAPALAAADVG"
     assembly_gap    4109..4264
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4265..4840
                     /locus_tag="PK264_12610"
     CDS             <4265..4840
                     /locus_tag="PK264_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HRD76758.1"
                     /translation="LSGLAMGLAAFGYLSPIAGALAQEGIDLAVILNALRALAPGRRT
                     AVNALTADAARAIAAEHSDLTATLDRLRVITVALDTTSPGESRVLLDEADRLIKGTIV
                     RHEQADERELYPRLRRHLADNHGLAAMSVAHREILHLSRLLTRMVASSTHELLDPPTI
                     REMQRVIVTLEALVRLHTAEEDEIVAAATHA"
     gene            5039..6655
                     /gene="ccoN"
                     /locus_tag="PK264_12615"
     CDS             5039..6655
                     /gene="ccoN"
                     /locus_tag="PK264_12615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HRD76759.1"
                     /translation="MSVAAAIGSGILFGFVGLAWMFAGLDLPIRFHGFVLFLGALIGI
                     GYVLYNADKPINRDAYFDGPIRFATLAAVFWGVAGFIVGDVIAWQLAVPALNLDLPWT
                     SFGRLRPLHTSAVIFAFGGNVLLATSFYVVQRTCHTRIAGRFAPWFVIWGYNVFIVIA
                     GTGYLLGVTQGKEYAEPEWYADLFLTIVWVVYLLVFLGTLMKRKEPHIYVANWFYLAF
                     IVTVAMLHLVNNATLPVSLLGSQSYIIYAGVQDAMTQWWYGHNAVGFFLTAGFLGIMY
                     YFVPKRVERPVYSYRLSIVHFWTLIFLYIWAGPHHLHYTALPDWAQTLGMTFSIMLWM
                     PSWGGMINGLMTLSGAWDRLRTDAVVRLLVVSVAFYGMSTFEGPMMSIKAVNSLSHYT
                     DWTIGHVHSGALGWVAMVSFGAIYCLVPWLWGRKGLYSMRLVEWHFWVSTIGILLYIT
                     AMWVSGITQGLMWRAYDRLGLLQYSFIETVEAMKPYYVIRAIGGLLFVLGALFMVFNI
                     WKTVRGDEPVDLLDQPRNAAAPELRPVAAE"
     gene            6673..7401
                     /gene="ccoO"
                     /locus_tag="PK264_12620"
     CDS             6673..7401
                     /gene="ccoO"
                     /locus_tag="PK264_12620"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HRD76760.1"
                     /translation="MNHEPFEKNSIILLIGILIMVSIGGIVQIAPLYWVDSTIEKVKG
                     MRPYTPLEQTGRDIYIREGCYGCHSQMVRTLRDEVERYGHYSLAAESMYDHPFQWGSK
                     RTGPDLARVGGRYSDLWHAEHMINPRAVVPESIMPGYPWLATTLVDVDKVEARIKTLR
                     TVGVPYTDEMIASVKADVVAQVRPDTRPSRDLLKRYPKAKVAKFDGKAGTAPTELDAL
                     IAYLQVLGTLVDFASFDASGPGRR"
     gene            7412..7588
                     /locus_tag="PK264_12625"
     CDS             7412..7588
                     /locus_tag="PK264_12625"
                     /inference="COORDINATES: protein motif:HMM:NF017366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HRD76761.1"
                     /translation="MSYDTIATWSQVTSLLMFVAMFLAVAAYVLWPRNRERLEAAHRR
                     ALDLPASRNEKGRK"
     gene            7588..8460
                     /gene="ccoP"
                     /locus_tag="PK264_12630"
     CDS             7588..8460
                     /gene="ccoP"
                     /locus_tag="PK264_12630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HRD76762.1"
                     /translation="MAKSTREIDTVTGVETTGHEWDGIKELDKPLPKWWLWTFYACCI
                     WAFGYWIVYPAWPTLSGYTPGIWNYSQRHVVAREVEAGKAATKGVRDKIAAMPVADIA
                     KSDDTARFAMAAGAAAFQANCAACHGRSATGFVGYPNLVDDEWIWGGTIGDIYTTILY
                     GIRSEHKQTRLSQMPRYGIEKLLDEAKIGEVADYVLSLSGRAADAASVAAGAKTFAAQ
                     CADCHGADGKGKADRGAPNLTDPIWLYGSDREAILESIRTGRGGQMPLWVGRLDDVVI
                     KSLAVYIHSRGGGK"
     gene            8796..>9755
                     /gene="ccoG"
                     /locus_tag="PK264_12635"
     CDS             8796..>9755
                     /gene="ccoG"
                     /locus_tag="PK264_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HRD76763.1"
                     /translation="MAVENARAVNRKADRELYASREKIHPRLARGTFRRLKWLVMLVT
                     LGIYYALPWLRWNRGPDLPSQAVLVDMANNRYFFFFLEIWPQEFYFITGLLILAALAL
                     FMMTSMAGRVWCGYTCPQTVWTDLMIAVERFWQGDRNARIRLDASPWSASKVFKKVAT
                     HASWLLIGLATGGAFVFYFRDAPTLARELLTGDAPYIAYLFLVIFALTTYVLGGIARE
                     QVCIYMCPWPRIQGAMVDHETLLVTYKSERGEPRGPHKKGQPWEGRGDCIDCKACVAV
                     CPAGIDIRDGAQLECIQCALCIDACNEIMTKVGRPRGLVAYSTI"
     assembly_gap    9756..9773
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9774..10240
                     /locus_tag="PK264_12640"
                     /pseudo
     CDS             9774..10240
                     /locus_tag="PK264_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110433.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
     gene            10256..10774
                     /locus_tag="PK264_12645"
     CDS             10256..10774
                     /locus_tag="PK264_12645"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HRD76764.1"
                     /translation="MSPTVLPSAERRGGLQGRHVLITFLAFFAAIFAVNGALLYFALK
                     THSGLVAVEPYRKGLAYNDRIAADATQSALGWKAEAALSSGGALTVAMADRDGKPVAG
                     LKVTGLFGRPSTSKDERRLALIETAPGRYSADLGALSTGAWRIDIEALRPATDLAPDA
                     LYRARRRLWLTP"
     gene            10852..12225
                     /locus_tag="PK264_12650"
                     /pseudo
     CDS             10852..12225
                     /locus_tag="PK264_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(12349..12529)
                     /gene="eno"
                     /locus_tag="PK264_12655"
                     /pseudo
     CDS             complement(12349..12529)
                     /gene="eno"
                     /locus_tag="PK264_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589557.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; incomplete; partial
                     in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
BASE COUNT         1904 a         4353 c         3861 g         2073 t
ORIGIN      
        1 ggcctcgtag agcttgaggc cgaggcgcgg gtcgcagccc gcgagtgctt gcaagagggc
       61 gaccggcagg cagcgcagca cggccgtgcc ggtcgcctgc gcgttgtggc cgtgcgtcgc
      121 gagcgcgccg aggccgatca cgtcgcccgg gtaggcgaag tcgatcacct ggcggcggcc
      181 gtcgggcatg gtgcggtaga ggacgacgtg gccagcctcg acccggtaga cgtggctcgc
      241 cggatcgccc tcgcagaaga ggtgctcctt ggcgccgagc gtgcggatcg gtgccgtctc
      301 gaagcgctcg gcgagcgtgt cggatttcgc ctgcgacgcc aggcgcggcg tggtccggtt
      361 ctgcgcgggc ggcgcatgcc cggcagcggc gacactctcg gcggcgacag gcgcgaaggc
      421 agcgaccagc atggtgtccc catcgttctt ggcgtttgac cgatggggtg acatgacggc
      481 ccggccgggt taggcgtatg ccgtccaggt gagactgtgt gagagtgtgt gagagctgca
      541 gcgatcgcgc gaagccgacc ttcacccctg ccgatcaagc aacgagagtg agccgtcaat
      601 gcccgaccgg cagccgaagc acatcctggt cgtcgacgac gaggcgcctg tccgcacgct
      661 gctgcgcgac tgcttcgaga ccgagggcta tgtggtcgca gaagccgccg acgccgcagc
      721 agccaaggcg cacatctcgg cggctccgcc gagtctcgtc accctcgatc ttaagctcgg
      781 cggcgacgac ggcctggttc tcgcccgcga gatccgcgcc atctgcaatg tgccgatcgt
      841 catgatctcc gggaaaggtg acaccatcga ccgtgtcgtc ggcctcgagc tcggtgccga
      901 cgactacatc tccaagccgt tccatgttcg cgaggtgctc gcacgggtga aggccgtact
      961 gcgccgctac gagacgggca ccacagcgct gccgggacaa ctggcaccgc cgccggctgc
     1021 tgccggcacg accttgactt cgtctcccgt gatcggcggc gcggcggacg agcgctacga
     1081 gctcgccggc ctcgtcctgg acatcccgcg acgcgagctc cgcgacagcg atggccagat
     1141 catcgagctg acgacggccg agttcaacat gcttgcgatg ttcgcgccgc gcccgcaccg
     1201 cgtcctctcg cgcgacaaca tcatggacct cctcaagggc cacgagtggt cgccgttcga
     1261 tagatcgatc gacagcctcg tcgtccgcct cagacgcaag atcgagccat ctcccgacac
     1321 gccccgcatc atcaagacgg tgcgcggcgt cggctatgtg ctcgcctcgg atgtgaaaag
     1381 gctctgacgg tcaggtcttc ggcacgccgg ctccggcctc gagcacctga cgcaggcgct
     1441 cgacaagagc cgagatctcg ctcgcccgtg cacgcccgtg attcttcaga tactcggcta
     1501 ccgctgtgtg gctctgccga ccgaccagca cgccgtcgcg cacctcgaga tcggacttgg
     1561 caaactcggc aagcgcgccg tggcacttgc tgcagcggcg ctcgtaggcc gcatcaccgg
     1621 ccgattgggc agccgcagtc gaggcagtaa gattcagcgc ggcgaccagt gcagcgaagc
     1681 cgatgctccg caactgaagc tgccgcgcat tgcgtgcttg gcgttgtccc gtcatctcgt
     1741 tcccgtctgg gtatcgtatg cggtcatcgc gcacgaccgc ccgcttcgcg tgatgtgtcc
     1801 cacagcttgc gcgacccgcg cctgcggcca cagctcgcac ctcggtcaga cactttcctt
     1861 gacctcggtc aagcggttgc ttgcaaatac ggtcttcact cgatcgaccc gttcatggga
     1921 ggtggcgatg ccgcgtttcc gcttggtgcc ctggctccgc tcatcatacg cgcgcgcggc
     1981 tggccgcctg actccggcct tccccggcct cagcgacgaa cagatcgagc gactgctcgc
     2041 cgatatcgac gcaacccttg ccgacatgcg gcacatgacg cgcactcatg cggggccgaa
     2101 catcctgatg cgcgagcggc tcgccgcgcg cggcgccgat gtcgacgccg tggcacgagc
     2161 cgcgccatca ctcatcgcaa ctctcgaggc cacttgcgac acctgtgccc actggcgtcg
     2221 ctgtgcccgc gaccttgccc gatcgccgag ccgaacagat ggcgaggact actgcccgaa
     2281 ctcgccgcat atcgacggaa tcgagccccg tcgacgtggc gagcccactt ccgggtgttg
     2341 accgttgtca aggctcactc ctcgtgatca accacaccgt cccattcgtg cacaacgtgg
     2401 gaagtgagtg cagacgatcg cgctcgacat ttcgggcgag cgaggagcgc ttgtacgcat
     2461 gaccgatgcc tcacgacaag gccatgtgga tccggctcga acggctgtcc gccaggtctc
     2521 gtggagtacc cgtctgatgc attgggtagc ctcggcgggc gaatccgatc tccgccgcct
     2581 gctcgtcgcc tacgtcatcc tcgggctggt cgctggcctc gccgcatacg gtctcggtca
     2641 gccccgggtt gcgcatgccc tgtggatcgt cgcgacgctc ccggtcgtga cgactctcgg
     2701 cgcctcgatc gtccgcgatt tcgcagccgg gcgcgccggc gtcgatgcca tcgccttcgt
     2761 ctcgatgacg acggcgctcg tcctcggcga gccgctggcc gcgaccgtgg tcgccgtcat
     2821 gtactcgggc ggcatgctgc tcgaggatct cgccgtcgcg cgcgccaacc gcgacctccg
     2881 tctcctcatc gaccgcgcgc cgcgaattgc gcacctcctg gccggcggga cggtccgcga
     2941 cgtcggcatc gatgccgtcg tcatcggcga cacgctgctc gtgcgctccg gcgaggtgat
     3001 ccccgtcgac ggcaagctgc tcgccgaccg cgccctgatt gacgaggcag cattgacggg
     3061 cgaagcgctg ccgctcgcgc gccttcgggg tgatcctctc cgcagcggca ctctcaatgc
     3121 gggaccgaca ttcgagatgc gcgcgactgc cgccgcagac tcgagcacct acgcggggat
     3181 catccgcctc gtcacggcgg cgcagactgc caaggcgccc ttcatgcggc tcgccgaccg
     3241 gttcgcgctc atcctgctgc ccgacaactc catgaagctg ttgacccccg cgctnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncccc tgtccgctga tccttgcggc
     3361 gccgatcgcc ttcatttccg gaatctcgcg ggcggcgcgg cgcggcatcc tcgtcaaggg
     3421 cagcgccgcc atggagcggc tcgcaggcgc caccacgctg ctcttcgaca agaccggcac
     3481 gctcaccatc ggcggcgccc ggctcgccgc agtcgaaacc gcgcccgggg tgacgggcga
     3541 tgaggcactg gcgctcgccg cctccctcga gctcggctcg cccaatgtgg tcgctgcgtc
     3601 aatcgtcgcg gcggcccgcg agcgcgggct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncctgggcg aaacgcgccc tccagcgcgc
     3781 gacgtggcgc aatgccctca ccgtcttcat tgccgtcgac gggcggccgg cagcggccct
     3841 cctgcttgcc gacgagcttc gccgcgacgc cccccgcgcc atccgcgcgc tgcgcgaggc
     3901 cggaatctcg cgcctcctga tggtcacagg cgaccggccc gatgctgccg aggccgtcgg
     3961 cacggccctc gggctcgatg ccgtgctcgc cgagcgcacg ccgtcggaga aggtcgaggc
     4021 ggtggcctcc gagcagagcc gggcccgcac gatcatggtc ggtgacggca tcaacgatgc
     4081 gccggcactg gccgccgccg acgtcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnctttcg ggcctcgcga tggggctcgc ggcattcggc tacctctcgc cgatcgccgg
     4321 cgcgctcgcc caggaaggca tcgatctcgc cgtcatcctc aatgcgctcc gcgccctcgc
     4381 gcccggacgc cgtacagccg tgaatgctct cactgccgat gcggcgcgag ccattgctgc
     4441 cgagcattca gacctcactg ccacactcga ccgcctgcgc gtgatcactg tggcgctcga
     4501 caccacttca cccggggagt cacgcgtact tcttgacgaa gcggatcgcc tgatcaaagg
     4561 gacgatcgtg cgtcacgagc aggccgacga gcgtgaactc tacccgcgac tgcggcgcca
     4621 tcttgccgac aaccatggtc tcgcggccat gagcgtcgca caccgcgaga tcctccacct
     4681 ctcgcgattg ctgacccgga tggtggcaag ctcgacccat gagttactcg acccgccgac
     4741 gatccgggag atgcagcgcg tcatagtgac actcgaggcg cttgtgcggc tgcataccgc
     4801 cgaagaggac gagattgtcg ccgcggctac ccacgcctga gggcgcaaca gtgagctcct
     4861 tcgggtccgg ccgttacgtc gcagcgcgac caaaggcgcc gtgccacagc cgctggcgtt
     4921 gacaaagatc aatgagtggc cggccccggg gcctgaatgt gcactgcacc atcagcccag
     4981 actctcgggg ggcccccaca tgcaccagcc tgcgaaaaca agcgccgagt ggcacgcaat
     5041 gagcgttgcc gcggcgattg gcagcggcat cctgttcggc tttgtcggct tggcatggat
     5101 gtttgccggc ctcgacttgc cgatccgctt ccacggtttc gtcctcttcc tcggcgcgct
     5161 gatcgggatt ggctacgtcc tctacaatgc cgacaagccc atcaatcgcg acgcctactt
     5221 cgacgggccg atccggttcg cgacgcttgc cgccgtcttc tggggcgttg ccggcttcat
     5281 cgttggcgac gtgatcgcct ggcaactcgc cgtgccggcc ctcaacctcg atctcccctg
     5341 gacgagcttc gggcgtctcc gtccgctgca cacctcggcc gtgatcttcg ccttcggcgg
     5401 caacgtgctg ctggcgacgt cgttctacgt cgtgcagcgt acctgtcaca cgcggatcgc
     5461 aggtcgcttc gcaccctggt tcgtgatctg gggctacaac gttttcatcg tcatcgccgg
     5521 cacgggctat ctgctcggcg tcacgcaggg caaggagtac gccgaacccg agtggtacgc
     5581 ggacctgttc ctcaccatcg tctgggttgt ctacctgctc gtcttcctcg gcacgctgat
     5641 gaagcggaag gagccgcaca tctacgtggc aaactggttc tacctcgcct tcatcgtcac
     5701 cgtggcgatg ctgcacctcg tcaacaacgc gacgctaccg gtgagcctgc tcggctcgca
     5761 gagctacatc atctacgcgg gcgtgcagga tgcgatgacg cagtggtggt acggccacaa
     5821 cgcggtcggc ttcttcctga ctgccggctt cctcggcatc atgtactact tcgtgccgaa
     5881 gcgggtcgag cggccggtct actcctaccg cctgtcgatc gtgcacttct ggaccctgat
     5941 cttcctctac atctgggccg gtccgcacca cctgcactac accgcgctgc ccgactgggc
     6001 gcagacgctc ggcatgacgt tctcgatcat gctgtggatg ccgtcgtggg gcggcatgat
     6061 caacggcctc atgacgctct cgggtgcctg ggaccggctc cgcaccgacg ctgtcgttcg
     6121 cctcctcgtc gtctcggtcg cgttctacgg catgagcacg ttcgagggcc cgatgatgtc
     6181 catcaaggcc gtcaactcgc tctcccacta cacggactgg acgatcggcc acgtgcactc
     6241 cggcgccctc ggctgggtcg ccatggtgtc gttcggcgcg atctactgcc tcgtcccgtg
     6301 gctgtggggt cgcaagggtc tctactccat gcgcctcgtc gaatggcact tctgggtctc
     6361 gacgatcggc atcctgctct acatcacggc gatgtgggtg tcggggatca cgcaaggcct
     6421 gatgtggcgc gcgtacgacc gcctcggtct cctccagtac tcgttcatcg agaccgtgga
     6481 ggcgatgaag ccctactacg tgatccgcgc catcggcgga ctgctcttcg tgctcggcgc
     6541 cttgttcatg gttttcaata tctggaagac cgtccgcggc gatgagcccg tcgatctcct
     6601 cgatcagccg cgtaatgccg ccgcacctga gctgcgccct gtcgccgccg aataaccgaa
     6661 agcacccgag caatgaacca cgaacccttc gagaagaact cgatcatcct cctgatcggg
     6721 attctcatca tggtgtcgat cggcggcatc gtgcagatcg cgccgctcta ttgggtcgac
     6781 agcaccatcg agaaggtgaa gggcatgcgc ccctacaccc ccctcgagca gaccggccgc
     6841 gacatctaca tccgcgaggg ctgttacggc tgccacagcc agatggtgcg cacgctccgc
     6901 gacgaggtcg agcgctacgg ccactacagc ctcgccgcgg agagcatgta cgaccatccg
     6961 ttccaatggg gatcgaagcg cacggggcca gacctcgcgc gcgtcggcgg tcgctactcc
     7021 gacctctggc atgccgagca catgatcaac ccgcgcgcgg tcgtgcctga gagcatcatg
     7081 ccgggctatc cctggctcgc cacgaccctc gtcgatgtcg acaaggtgga ggcgaggatc
     7141 aagacgctgc gcacggtcgg cgtgccctac acggacgaga tgatcgcatc tgtgaaggcc
     7201 gacgtcgtcg cccaggtccg ccccgacacg cggccctcgc gtgatctcct gaagcgctac
     7261 ccgaaggcga aggtcgccaa gttcgacggc aaggccggca ccgcgccgac cgagctcgac
     7321 gcgctcatcg cctatctgca ggtgctcggc acgctcgtcg acttcgcgag cttcgacgcc
     7381 agcggcccag ggcggcggtg aggaggcagc catgtcctat gacaccatcg caacctggag
     7441 ccaggtcacc tcgctcctga tgttcgtcgc catgttcctg gccgtcgcag cctacgtgct
     7501 ctggccacgc aaccgggagc gcctcgaggc ggcgcaccgg cgcgcgctcg atcttccggc
     7561 ctcacgcaac gagaaggggc ggaagtgatg gccaagagca cgcgcgaaat cgacaccgtc
     7621 actggcgtcg agaccaccgg acacgaatgg gacggcatca aggagctcga caagccgctg
     7681 ccgaagtggt ggctgtggac cttctatgcc tgttgcatct gggccttcgg ctactggatc
     7741 gtctatccgg cctggccgac gctctccggc tatactcccg gcatctggaa ctacagccag
     7801 cgccatgtcg tcgcccgcga ggtcgaggcc ggcaaggcgg caacgaaagg cgtgcgcgac
     7861 aagatcgcgg ccatgccggt cgccgacatt gcgaagagcg acgatacggc gcgcttcgcc
     7921 atggcggccg gagccgcggc ctttcaggcg aactgcgcag cctgccatgg ccgctcggcg
     7981 accggattcg tcggatatcc gaacctggtc gacgacgagt ggatctgggg cggcacgatc
     8041 ggcgacatct acacgacgat cctctacggc atccgctcgg agcacaagca gacgcgcctc
     8101 tcacagatgc cgcgctacgg catcgagaag ctgctcgacg aggcgaagat cggcgaggtc
     8161 gctgactatg tcttgagtct ctcgggtcgc gcggccgatg ccgcgtcggt ggctgccggc
     8221 gccaagacgt tcgccgcgca atgcgccgac tgccatggcg ccgacggcaa gggcaaggcg
     8281 gaccggggag ctccaaacct caccgatccg atctggctgt acggcagcga ccgtgaggcg
     8341 attctcgaga gcatccgcac cggacgtggc ggccagatgc cgctctgggt cggccgcctc
     8401 gacgacgtcg tcatcaagtc gctcgccgtc tacattcaca gtcgcggcgg cggcaagtag
     8461 ccgaattggg aggtcgatgc atgtgctgct gtggttgcca gcacgaaagc ctcgctgaga
     8521 tcgaagctcg catggccggc cacgaaccgg ccctgcgatc agacgaggcg atcctgactg
     8581 gcaccacggc ttccgagcga gccggcggcg gcctcatccg ccgcatgctt cgcgccttga
     8641 gggatggtct cggccgcgtc ggcgccttat cggttgccac gacagcgcgc cggtgacggc
     8701 agagagataa cgagaaggcg gagcacgtga gccggcccga ggatttcatc atcgccagca
     8761 gtgcggcggc gccggcggca gcagcggccg agggcatcgc cgtcgagaac gcacgcgcgg
     8821 tcaacaggaa ggccgaccgc gagctctatg cctcccgcga gaagatccat ccgcgcctcg
     8881 cccgtggcac gttccgccgt ctcaagtggc tcgtgatgct ggttaccctc ggcatctact
     8941 atgcgctgcc atggctgcgc tggaaccgcg gccccgacct gccgagccag gccgtgctcg
     9001 tcgacatggc gaacaaccga tatttcttct tcttcctcga gatctggccg caggagttct
     9061 atttcatcac cgggctcctg atcctcgcag cccttgccct cttcatgatg acgtcgatgg
     9121 ccggacgcgt gtggtgcgga tacacctgcc cacagacggt ctggaccgat ctgatgatcg
     9181 cggtcgagcg cttctggcag ggcgaccgca acgcgcgcat ccgcctcgat gcctcaccgt
     9241 ggagcgcatc gaaggtcttc aagaaggtcg cgacacatgc ctcgtggctg ctgatcggtc
     9301 tagcgacggg cggcgcgttc gttttctatt ttcgcgatgc gccgacgctc gcccgcgagc
     9361 tcctgaccgg cgacgccccc tacatcgcct acctcttcct cgtcattttc gcgctcacga
     9421 cctacgtgct cggcggcatc gcccgcgagc aggtctgcat ctacatgtgc ccttggccgc
     9481 gcatccaggg cgccatggtc gaccacgaga cgctgctcgt cacctacaag agcgagcgcg
     9541 gcgagccgcg cggtccgcac aagaagggac agccctggga aggccgcggc gactgcatcg
     9601 actgcaaggc ctgcgtcgcc gtctgccccg ccggcatcga catccgcgac ggcgcacagc
     9661 tcgagtgcat ccagtgcgcc ctctgcatcg atgcctgcaa cgagatcatg accaaggtcg
     9721 gccggccgcg cgggctcgtc gcctattcga cgatcnnnnn nnnnnnnnnn nnncctgcgg
     9781 caagaacgcg ccactccgcc tcgtgcgccc gcgcaccatg ctctatgcgg gcctcgtcac
     9841 gctggtgagc gccattctgc tcggcgcctg gttcatgcgc accgtgctcg agatcaacgt
     9901 gctggccgat cgcaacccgc cctacgtgcg cctctcggac ggcagcatcc gcaacggcta
     9961 caccgtgaag atcctgaaca agctgcacga gccgcacacc ttcgagatca cgatccgcgg
    10021 gctcgatggc gccgtgctct cagcggtcgg cctcgagggc ggcgcgaagc cgcgtgtcac
    10081 cgtcccgacc gacgaccttc gcgagctgcg cgtcctcgtc acgctaccgg ctgcggccgc
    10141 gagccgcctc gccgaggcga cgaccgacta ccatgtcgtc atcaccgacg tcaccgagcg
    10201 acgcgaagcg acccggcgcg tcaagttcca gcggccctga tcagaggagg cgcacatgag
    10261 tccgactgtt cttccctcgg ccgagcgccg gggcggcctg cagggccggc acgtgctgat
    10321 caccttcctc gcgttcttcg cggcgatatt cgccgtcaac ggcgcgctcc tttacttcgc
    10381 gctgaagacg cacagcggac tcgtggccgt cgagccctat cgcaagggcc tcgcctacaa
    10441 cgaccgcatt gccgccgacg cgacacagag cgcgctcggc tggaaggcgg aggcggcgct
    10501 ttcgagcggt ggcgcgctca ccgtcgcaat ggccgaccgc gacggcaagc ccgtcgccgg
    10561 cttgaaggtc accggcctct tcggccgccc gtccaccagc aaggacgagc gccggctcgc
    10621 ccttatcgaa acggcgcccg gccgctactc agccgacctc ggcgcactct cgaccggcgc
    10681 ctggcgtatc gacatcgagg cactgcgccc ggcgaccgac ctcgcccccg acgccctcta
    10741 tcgcgcacgg aggcggctat ggctcacgcc gtgacgaccg agacgacgct ggtcgtcgag
    10801 aacatgcact gcggcggatg cctctcgaag gtcgagcgcg cgctcggcgg aatgcgcggc
    10861 gtcgccaagg cccgcgccaa cctctcgacg aagcgcgtga cagtggagca cgatccggaa
    10921 agagcatcga ccccgagcct cgtcgatgca cttgccgccg ccggcttcaa ggccgccgcc
    10981 ctcgcaggcg ccgcaaccgc ccctgccgac aatgccggtc gcgacctgat gcgccgcctc
    11041 gcggtcgcgg gttttgcggc agcaaacatc atgctgctgt cggtctcggt ctggtccggc
    11101 gcggacatgt cgtcgagcct gaaggcgctg ttccactggc tctcggcgct gatcgccatg
    11161 ccagcgatcg cctatgcggg ccagccgttc ttccgctcgg cggcaacggc gctcaaggcg
    11221 cgctccgtca acatggacgt gccgatctcg ctcggtgtga cactcgcgac ggcaatgagc
    11281 gtctaccaga cgatccgcgg cagcgatcag gtctacttcg acgccgccgt gacgctgctc
    11341 ttcttcctgc tcgtcgggcg cgcactcgac aaccacgtgc gcgcgcgagc gacgagtgcc
    11401 gcgcagaacc tgctcggcct gcgtgcgagc ttcgcgaccg tactagaggc ggatggcggc
    11461 agccggcgga tcgcggcgac cgaactcgcg cccggcatgc gcatcctgac ggccgccggc
    11521 gaacgatttg ccgtcgacgg tcgcgtcgtc acgggctcga gcgagatcga cgagagccta
    11581 atcaccggcg agacgaagcc ccgcgcgata acggccggcg cgtcggtcta tgccggtacc
    11641 ctcaacctcg gccagcccgt caccacggaa gcgctcgcca ccaacgagcg caccctcgtc
    11701 gccgagattg cacgcctgat gtcggcggcc gagcagggcc gcgcgcgcta tgtgcgcctc
    11761 gccgaccgcg ccgcgcggct ctatgcgccg gccgtgcacg tactgagcct tgggacgttt
    11821 ctcggttggc tcttcctcgg cttcggctgg gaatcggcac tcaccgccgc catcgcggtc
    11881 ctcatcatca cttgcccgtg cgcgctcgcg ctcgccgtgc ctgccgtgca ggtcgcggca
    11941 acaagccgcc tctttgccaa gggcgtgctc gtcaagagcc ccgatgcgct cgagcgcatc
    12001 gcccagatcg acatggtcgc cttcgacaag accggcacgc tgaccctcgg tgagccgcgc
    12061 ctgtccgaag cctcggcgat cacggacgag gttctcgcgc gcgccgccgc gctcgggttg
    12121 cagagccgcc acccctatgc gcgtgccgtc gtgcaggccg cccgtcagcg cggcctgacg
    12181 ctcccggcgc ttgccgacgt caaggaaaca ccggggctcg gcctctctca tgccagtccg
    12241 cacggcgagg aacgcctcgg ctctgccgcg tggtgcggcg ctgccgacgg ggccggcgcc
    12301 tcgctctggt accgccgcgg tgacgcggca ccgatcgcca tcccgctcga ggatcgcgtt
    12361 cgcgccgaga ttgctcttgt gcgccgttcc gtcgagcgcg agcatcttct cgtcgatggc
    12421 tttctggttg agcgcatact cgccgatcag ggcttcggcg attgctccgt ttaccgcgct
    12481 gaccgccttc aaaacgcctt tgccgccata ccggctctta tcgccgtcg
//
LOCUS       DAORNH010000114        12510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3810_length_12510_cov_2.567965, whole genome shotgun sequence.
ACCESSION   DAORNH010000114 DAORNH010000000
VERSION     DAORNH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_12510_cov_2.567965"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PK264_12660"
     CDS             complement(<1..857)
                     /locus_tag="PK264_12660"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76765.1"
                     /translation="MRIARRPIAAAALVLAVLVAGPLGNDRVRAQTPGRPPGSDIVLP
                     NASTDRHVKRCLFLALRRQNPKLDAMTTTVENAGAILDIPIVLDAVATCRLAAAKFPA
                     DKDVIIATYTATEVLNVLLFGITDHPGTAAEALAKVRRMDVRPDDPLFGRLVMFALGS
                     AYHHGIGVTANISEAIEWYRKAGAGGDRVSMRELARIFMTGSGVAVNVELAARLLLAA
                     IIAGDEHERDELVASQGAALTKEMRTAIQNELKRLGNDSVAPTGTFDKETLAALSKLT
                     VKKEAAPQD"
     gene            complement(950..1693)
                     /locus_tag="PK264_12665"
     CDS             complement(950..1693)
                     /locus_tag="PK264_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD76766.1"
                     /translation="MISLSLWQIGLIAVTAFGAQVVGGLAGYGTGLLMPLVLVPMIGA
                     EAVVPIISVSSIITNVTRAIVFRESVDYRRAAIITLAALPTTLAGAWCYTLLSSRGAT
                     LVIAGVLLAVVILRRWIAALDMRISDRGTAAAGVAYGFLTGGSTGVGVILLSILMAAG
                     LSGTQVIATDALVTIVLGIAKSGVFAAAGVLSRDILLIAVLVGAMATPGTLLARYLAK
                     EMSLKLHDLMIEIAIVVGALVLIWRALMS"
     gene            complement(1690..2691)
                     /locus_tag="PK264_12670"
     CDS             complement(1690..2691)
                     /locus_tag="PK264_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76767.1"
                     /translation="MPTLSVIVTNGDALAATLRIHCVDADIIPWRDVLWEGPVPANFD
                     DRELAEVRARHLASAFSSRFRDDLAMLLERDDQFATARWTDSITLLFEPDLTDQLQLL
                     QCLDRIARWSDTGAAVCLAECRAPLRTAADTRAALLGARALTTGILALGRAAFEAFRA
                     PDPIALLALLAADTSPLPDLRNALIDLLGDLPAPGSGLSASEAAILTCLEQGACTPAK
                     LFAELARRGDPLAQRGDWCVFAIISRLASTPPAAIAGLDGCFPIDGSTNERGAFLDSR
                     ISLTDCGKALLGDELDLTQLAPPHRWLGGMQLTPENLWRWSRTDMHLIPPDRLLAAP"
     gene            complement(2770..3087)
                     /locus_tag="PK264_12675"
     CDS             complement(2770..3087)
                     /locus_tag="PK264_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HRD76768.1"
                     /translation="MDVDHEADLKKYAPSADSKHVKAVIKHLGIALKSFDSSMVSCSS
                     KDELERVREQWLKKKLKLTASDADLDKSIKAVCEKMKGDKNKSRVAFYYLLADHHKKL
                     DAL"
     gene            3336..3815
                     /locus_tag="PK264_12680"
     CDS             3336..3815
                     /locus_tag="PK264_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76769.1"
                     /translation="MAILLSGQAMTWWLWIKLVMLSGGAVAIAAFGARAQDQSGQSMS
                     GAEIQAEFVGRRLAGIYPHGTVWSETVVKDGTTDYLERGVRNAGRWRIEGNLFCFSYA
                     RLLAGGCFEVIRLSPNCFDLYAWPSLAAPTDAPRRKSYNGKMWLADQPATCDDKPIV"
     gene            4089..5261
                     /locus_tag="PK264_12685"
     CDS             4089..5261
                     /locus_tag="PK264_12685"
                     /inference="COORDINATES: protein motif:HMM:NF021633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HRD76770.1"
                     /translation="MLAHGQDSPAVARSQDRDLRIDFFRGLALLFIFIDHIPGNTLGH
                     FTLRNFAFADAAEAFVLIAGISAAFAYGKGLDRPGVSGMKPLASRLVKIYQSHLLLVA
                     LGVALIMTAAWYFENPLYIEHINLTPFWYDPFGALWKTALLYHQIGYLNILPLYIVLL
                     LWLPILWKLLRIHLALGLATSVGIWLLARQLRLNLPAYPDAFGWYFNPLTWQLLFTLG
                     VTVGLLARRGVALPRSRWLIGVAALYAAAALIVMAPWVHISGLEKARLVPYDAFGHFW
                     KTEMPLQRLLSILAVAYLIAVTVRPNAGWFSAWPARIVIACGRNALDIFFVGTLLSFA
                     GFVVLMEAGRSWGVQLIVNAAGIAVLGWLGWYLTECKQRVVKSPAPASTVVPAVDR"
     gene            5354..6040
                     /locus_tag="PK264_12690"
     CDS             5354..6040
                     /locus_tag="PK264_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRD76771.1"
                     /translation="MARFKQPLPAITRVAKDGSYDIKIVALGSSSTAGSGASNPNASY
                     PARLDAELDRLFPGRDFHVANFGTGGQLASDMLERIDRDIIPLLPSLVIWQTGVNDAI
                     QDVGLENFRNTLRTGIAHLKSAGIDVILVDMQYYPRSERVAVYKDYLRAMRDVAEDLK
                     VALFRRHTIMHHLIVSGQYTSAELLAPDNFHLNDLSYGCLAKLIAEAITDQIRAGRRS
                     QSTLLPNDPY"
     gene            6084..>6863
                     /locus_tag="PK264_12695"
     CDS             6084..>6863
                     /locus_tag="PK264_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD76772.1"
                     /translation="MSDASVCDVLIAGSGAAAFSAAISARHKGLDVLMVEKAPVFGGT
                     TAYSAGVIWIPGTSHARRAGLDEPDGAALTYLESAIGNRLDKAKAAAFIAAGPEALDF
                     LEANTHVRYQVQTTWADYEPDLPGGSQGGRSLLPEPFDGRRLGARFNQLRAPISTMML
                     FGGMMVGRDDIPHMFNVTRSARSAVHVAGLVSRYARDRLRYRRGTRIANGNALIAALA
                     LSAEEKSIPLWLSSPLVRLVIEKGRVVGGVIAREGREVEVRA"
     assembly_gap    6864..6880
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6881..7770
                     /locus_tag="PK264_12700"
     CDS             <6881..7770
                     /locus_tag="PK264_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD76773.1"
                     /translation="AEDMRKRLYPHVARGAEHRALPPDSNTGDGIRLGLSAGGGFIED
                     VHHAAAWTPVSLVPQAGGGVVPFPHFIDRGKPGYIAVDRRGRRFVNEAKSYHVFVPAM
                     IEACRQDSAVECWLITDHRGIRRYGLGAAAPSPMPLGPHLRSGYIVRGGTLRELAERL
                     GIGPDGLAATVDAYNGPAREGRDVQFGKGGDAYQRFNGAAGHTPNPCVAPIEHPPYYA
                     VRLVPGDIGTFAGLKTDPQTRVLDGDGRAVAGLYAVGNDMASVMGGTYPGAGITIGPA
                     LTFGYIAGRTIAADAAGRS"
     gene            8658..9239
                     /locus_tag="PK264_12705"
     CDS             8658..9239
                     /locus_tag="PK264_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76774.1"
                     /translation="MPRSSATSVESAVDQVLPFCVVERRPRVAVFGKIILLSLAGIAG
                     AAPIVMVTALSASTPDAILRVADNPLVGLQFATGLLFWILLLGMPFWRFVRSAGIERK
                     VAIDAACVTIAERSLIGAREKVVPLTSYRGIAHRVRASLSGTRHELVLVGPSQRSNVV
                     IATAPRMSEGDIERMCNLLGLKPVPAREVITRR"
     gene            9328..9873
                     /gene="hpt"
                     /locus_tag="PK264_12710"
     CDS             9328..9873
                     /gene="hpt"
                     /locus_tag="PK264_12710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRD76775.1"
                     /translation="MNAKPAPDIEVLIPAEELSTRVDALALDIARLERDNLLVVSVLT
                     GSFVFAADLLRALNRSGLSPEVDFLTLSSYQRGVRSLGQVDILRDVSVDVRGRNILLI
                     DDILDTGRTLAFAKDLLTARGAASITTCVLLDKDVPRVVAIAADFAAFKCPDVFVVGY
                     GMDLSHRYRELPFVGRVVVRA"
     gene            9964..11391
                     /locus_tag="PK264_12715"
     CDS             9964..11391
                     /locus_tag="PK264_12715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRD76776.1"
                     /translation="MVFPTLAFLIFYCLVWPLSWGLVALRRHGAHKLSIIAASYIFYA
                     FWSWRFAFLLLSSVLLNWSVGRAIGSSTSPRLRRAHLAFGVLMNLAVLGFFKYYGWFA
                     EEMGVLLRLAGLSRDIPLFEVVLPVGISFFTFQGISYIVDLYRGDLDRARPLVDVMFF
                     ISFFPHLVAGPIVRAASFLPQLERPPRPDRVFVGLGMMLIVWGVWKKAVVANWLAVLL
                     VDKAFFDPAAYGALDLAAAIYGYAVQIYCDFSAYSDIAIGVAALLGYRFQRNFNQPYR
                     AVTLADFWRRWHMSLSSWLRDYLYVPLGGNKGGKVRTLRNLLLTMLLGGIWHGAAWKF
                     VAWGFLHGGALAIERLLGFRPREPHEPAPPAWKRVLPILIVFHFVCFAWIFFRADDMA
                     KALAVLAGLADWQRAPTLLTPLPLALIAFGLAIQFTPANTIERLDRIYQRLPTIVVGA
                     LVGAALLLIEAVGGDGSAPFIYFQF"
     gene            11403..11681
                     /locus_tag="PK264_12720"
     CDS             11403..11681
                     /locus_tag="PK264_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76777.1"
                     /translation="MSDWLPDPGLGTEDLEKGMRAGKARHVIPIALIAIAMLATFNSG
                     GLVKWSERLPSHEASLWVAEKAAAWHELMLELGPARVFEDVKVRLRIE"
     gene            complement(11668..>12510)
                     /locus_tag="PK264_12725"
     CDS             complement(11668..>12510)
                     /locus_tag="PK264_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76778.1"
                     /translation="LASIPVRPRIDKATKQSQADIVDPDRIAGALSALRGQTALIVAR
                     RDGERLVIQPQRGGERAILIKDVVGAATTGDVNLMVLASAAPRQPSGRNWLWLKVEVP
                     GLDHAAARPTVGDMLAALAGKSDTLAVAVTPVDADRIILKAQRIGDLGAEPSRVGAFL
                     DEMATAITGNVGIDAIEGWMTSRSRAGELRWRLVPGVPSIVQWGWLALIAAGLVALPV
                     ARRWFAALWPPEERSEYAAWRGYAAAVAMRCTLLVALFLPLAGLPALLAGALALSKRI
                     VTRS"
BASE COUNT         2028 a         4040 c         4070 g         2355 t
ORIGIN      
        1 caatcctgtg gcgcggcctc cttcttgacg gtgagctttg agagtgccgc gagcgtctcc
       61 ttatcgaaag tgcccgtcgg cgcgacgctg tcatttccga gccgcttgag ctcgttctgg
      121 attgccgttc gcatttcttt ggtgagggct gcgccctggc tggcgacaag ctcgtctcgc
      181 tcgtgctcgt caccagcgat gatcgcggca aggagcagcc gcgcggccaa ctcgacattc
      241 acagcgacgc cggagccggt catgaagatg cgggcaagct cgcgcatcga gacgcgatcg
      301 ccgccagcgc cagccttgcg ataccactcg atcgcctcac tgatgtttgc tgtaacgccg
      361 atgccgtgat gataggcaga gccgagtgcg aacatgacga ggcggccgaa caggggatca
      421 tcgggccgca cgtccatacg ccttaccttg gccaaggcct ctgcagctgt gcccgggtgg
      481 tctgtgatgc cgaagagcag gacattcagc acctctgtgg ccgtgtaggt cgcgatgatc
      541 acatccttgt cggccgggaa ctttgcggcc gccaatcggc aggttgcgac cgcatcgaga
      601 acgattggta tgtcgagtat cgcgcccgcg ttttcgaccg tcgtcgtcat ggcgtcgagc
      661 ttggggttct ggcgtcgcaa cgccaggaac aggcagcgtt tgacgtgacg gtcagtgctg
      721 gcgttgggga gcacgatgtc cgagcctggc ggccggccgg gtgtttgggc ccgcacgcga
      781 tcgttgccga gtggcccagc cacaagtacg gcgagaacga gtgcagctgc tgcgatcggc
      841 cttcgcgcaa tgcgcatggg ctcctcccgc tatgcatttg cgacatccac agcagtctag
      901 gccggagtgc gtgccaatag aagtccgcca cgccggtctt tggcgggcat cagctcatga
      961 gcgcccgcca gatgagcacg agcgctccga cgacgatagc gatctcgatc atgaggtcgt
     1021 ggagcttcag ggacatctcc ttggcgagat agcgcgcgag cagtgtgccg ggcgtcgcca
     1081 tggcgccgac caggaccgcg atcagcagga tgtcgcgcga gagcacgccc gctgccgcga
     1141 acacgcccga cttggcgatg ccgagtacga tggtgacgag cgcgtcggtc gcgattacct
     1201 gcgttcccga caggccggcc gccatcagga tagagagcag gatcacgccg acgcctgtac
     1261 tgccgccagt gaggaagccg taggcgacgc cggctgccgc cgtgccgcga tcgctgattc
     1321 gcatgtcgag cgccgcaatc cagcggcgca ggatcacgac agcgagcagc acgccggcaa
     1381 tcaccagggt tgcgccgcgg ctcgacagga gcgtgtagca ccaggcgcct gcgagcgtcg
     1441 tcgggagtgc ggcaagcgtg atgatggcgg cgcgccgata atcgacgctc tcgcggaaga
     1501 cgattgcccg tgtcacgttg gtgatgatgg agctgaccga gatgatcggc actacggcct
     1561 cggcgccgat catcgggacg agcaccagcg gcatcaggag acccgtgccg tatccggcga
     1621 gcccgccgac cacctgagcg ccgaaggccg tgaccgcgat cagtccgatc tgccagaggg
     1681 agaggctgat catggcgcag ccagcaggcg atcgggcggg atcaggtgca tgtcggtccg
     1741 gctccagcgc cagaggtttt cgggtgtcag ctgcatgccg ccgagccaac ggtgcggcgg
     1801 ggcgagctgt gtgaggtcga gctcatcgcc gaggagagcc ttgccgcaat ccgtaagcga
     1861 aatcctgctg tcgaggaagg cgccgcgctc gttggtcgag ccatcgatgg ggaagcaccc
     1921 gtcgagcccg gcaatggccg caggcggcgt agaggcaagc cgcgagatga tcgcaaagac
     1981 gcaccagtcg ccgcgctggg cgagcggatc gccgcgccgg gcgagctcgg cgaaaagctt
     2041 ggcgggtgtg cacgcgccct gctcgaggca ggtgaggatc gcagcctccg aagccgagag
     2101 accggagccg ggcgccggca gatcgccgag gaggtcgata agcgcatttc gaagatccgg
     2161 caggggagag gtgtccgccg caagcagggc gaggagggca ataggatcag gtgcgcgaaa
     2221 agcttcgaaa gccgccctgc ccagcgccag aatcccggtt gtgagggcgc gtgcgccgag
     2281 cagcgcggct cgggtatctg cggcggtgcg gagcggcgcg cgacattccg ccaagcacac
     2341 ggcggcgccg gtatcgctcc agcgagcgat ccgatccaga cactgcaaca gctgcagctg
     2401 gtcggtgagg tccggctcga agagcagcgt gatcgagtcc gtccatcggg ccgtggcgaa
     2461 ttgatcgtcc cgctcgagga gcatggcgag atcgtcgcgg aacctggagg aaaaggccga
     2521 tgccagatgc ctggcgcgga cctccgcgag ttcccggtcg tcaaaattgg ctggaactgg
     2581 accttcccac agcacgtcac gccaggggat gatgtcggcg tcgacgcaat ggatgcgcag
     2641 cgttgccgcg agggcatcgc cgttcgtgac gatcacggag agcgtgggca ttcttctgct
     2701 ctcgaataga acaaccctgg ccggctacgt tccgaccagg gtcgcatggc actcgcaggc
     2761 ctagccgtct cagagggcgt cgagcttctt gtggtggtcg gcgaggagat aatagaaggc
     2821 cacgcgcgac ttgttcttgt cgcctttcat cttctcgcag acggccttga tggacttgtc
     2881 gaggtcggca tcggaggctg tcagcttcag cttcttcttc agccactgct cgcgaacgcg
     2941 ctcgagctca tccttgctgg agcaggagac catcgagctg tcgaagctct tcagcgcgat
     3001 gccgaggtgc ttgatgacag ccttcacatg cttgctgtcg gcactcggag catacttctt
     3061 gagatcggct tcgtgatcga catccatgta tcgtctccct tgagctcagc gacggtcgat
     3121 tgtactcggc attgcgcact tcgacggatg atcattatcc gtcattcggc tgtccaactg
     3181 gaatccatcc aggtgaggag tgattccagg ctgacgccag cctacacctt cgaaggggga
     3241 aatcgagggc gaattatggc gcattggcta agttgtgggg cgtggatgca actcggacat
     3301 cggcgtgcca caatcgggcg ctgccagatg tcggcatggc gatccttctc tcggggcagg
     3361 caatgacgtg gtggctgtgg atcaagctcg tgatgctctc cggtggtgct gtcgccatcg
     3421 cggcgttcgg ggcccgggcg caggatcagt ctggccagtc catgagcggc gccgagatcc
     3481 aggcggagtt tgtcggtcgc aggcttgccg gcatctaccc gcatggcacc gtttggagcg
     3541 agacggtcgt caaggacgga acgaccgact atctcgagcg cggcgtcagg aatgccggcc
     3601 gctggcggat cgaggggaac ctcttctgct tttcctacgc gcgcctgctg gcgggcggtt
     3661 gcttcgaggt gatccggctc agccccaatt gcttcgacct ttatgcctgg ccgtcacttg
     3721 ccgcaccgac ggacgcaccc cgtcggaaaa gctacaacgg aaagatgtgg ctcgccgacc
     3781 aacctgcaac ttgcgatgac aagcctatcg tctgagacct gcataccgca cgagtccggc
     3841 gtccactgcg actgcgaacg gcatcgcgcc cgataccaat cgacatttcc gattgaacgc
     3901 gccaaatccc ccaaaatggc tccttcacgt cgcatcacag cttggtgaat ggggctcacc
     3961 cgtcttgttg cgtcgcaacg gatcgcatag ctcggtaacg ttggttcatt gcgatgttcg
     4021 agtttcagcc gctcgtcgag cggtcaaggg tgcgctcagt gcggcgcagg atgcttgagg
     4081 gggcgacgat gctggctcat ggtcaggaca gtccggcggt ggcgcgaagt caggaccgcg
     4141 accttcgcat cgacttcttc agggggctcg ccctcctgtt catcttcatc gatcacatcc
     4201 ccggcaacac gctcggtcat ttcacgctgc ggaacttcgc cttcgccgat gcggccgagg
     4261 ccttcgtgct gatcgctggc atctcggcag cgttcgccta tgggaagggg ctcgatcgac
     4321 ctggggtctc gggcatgaag ccgctcgcat ctcgcctcgt caagatctac cagtcgcacc
     4381 tgctgctcgt tgcgctgggc gtggcactca tcatgacggc ggcctggtac ttcgagaatc
     4441 cgctgtatat cgagcacatc aacctgacgc cgttctggta cgatccgttc ggcgcactct
     4501 ggaagacagc gctgctctat caccagatcg gctacctcaa tatcctgccg ctttacatcg
     4561 tgttgctgct ctggctgccg atactctgga agctgctgcg catccatctg gcgctcggcc
     4621 tcgcgacgtc agtcggcatc tggctcctgg cgcgtcagct ccggctcaat ctgccggcct
     4681 atccggacgc tttcggctgg tatttcaatc cattgacctg gcagctgctg tttacgctcg
     4741 gtgtcacggt gggtctgctt gcgcgacgtg gtgtggcgct gccgcgctcg cgctggttaa
     4801 tcggagttgc ggccctctac gcggctgcag cgctcatcgt catggcgcca tgggtgcaca
     4861 tctcgggcct cgagaaggcg cggctcgtgc cctatgacgc gttcggccac ttctggaaga
     4921 ccgagatgcc gctccagcgg ctcctcagca tccttgcggt tgcctacctg atcgccgtca
     4981 cggtgcgacc gaatgccgga tggttctcgg cctggccggc acgaatcgtc atcgcatgcg
     5041 gtcgcaatgc actcgacatt ttcttcgtcg gcaccctact gtccttcgct ggcttcgtcg
     5101 tgctaatgga ggccggacgt agttggggag ttcagctgat cgtcaatgcc gcaggcattg
     5161 cggtcctcgg gtggctggga tggtatctca cagagtgcaa gcagcgcgtc gtcaaatctc
     5221 cggcgccggc atccactgtc gtgccagcgg tcgatcgttg atgaggggtg tcgctggact
     5281 tgttcttgtt gcgtcgtcga tcgtgctcgg gagtgcaccg tcctttgcga aggggtgcac
     5341 cgtcccgccg gagatggcac gcttcaagca gccgttgccg gccatcacgc gggttgccaa
     5401 ggacggaagc tacgacatca agatcgtcgc gctcggctcg tcatcaacgg ccggcagcgg
     5461 cgcctcgaac ccaaacgcgt cctatcccgc gcggcttgat gccgagctcg accgtctctt
     5521 tccaggccgt gacttccacg tcgcgaattt cgggacgggt ggtcagctcg cctccgacat
     5581 gctcgagcgg atcgatcgcg acatcatccc gctgctgccg tccctcgtca tctggcagac
     5641 cggcgtcaat gatgcgattc aggacgtcgg cctcgagaat ttccggaaca cgctgaggac
     5701 cgggatcgcg cacctgaagt cggccgggat cgatgtcatc ctcgtcgata tgcagtacta
     5761 cccgcgttcc gagcgcgtcg ccgtctacaa ggactacctg cgggcgatgc gggatgtcgc
     5821 cgaggatctg aaagttgcgc tcttccggcg gcacaccatc atgcatcacc tgatcgtcag
     5881 cggtcagtat acgagcgccg agcttctggc tccggacaac ttccacctga acgatctctc
     5941 ctacggctgc ctcgccaagc tgatcgccga ggcgatcacc gaccagatcc gcgccggacg
     6001 ccggtcgcag agcacactac tcccgaacga tccctactga gcggcccggg actgtcctca
     6061 acggcaaaat caagggccgc gtcatgagcg acgccagcgt ctgcgacgtt ctcatcgcgg
     6121 gttcgggcgc cgccgccttc tccgcggcga tcagcgcgcg ccacaagggt ctcgacgtgc
     6181 tgatggtcga gaaggcaccg gtcttcggag gaacgaccgc ctactcggcc ggtgtcatct
     6241 ggattccggg gacgagccac gcgcgccgtg ccggcctcga tgaacctgat ggtgccgcgc
     6301 ttacctatct cgaaagtgcc atcggcaatc ggctcgacaa ggcgaaggcg gcggccttca
     6361 tcgcggcagg ccccgaggcg ctggacttcc tagaggccaa cacgcatgtc cgctatcagg
     6421 tgcagacgac ctgggcggac tacgagccgg acctgcccgg cggctcgcaa ggcggccgct
     6481 cgctgctgcc tgagcctttc gacgggcgtc gactcggcgc gcggttcaac cagctgcggg
     6541 cgcccatctc gacgatgatg ctgttcggcg gcatgatggt cggccgcgac gacatcccgc
     6601 acatgttcaa cgtgacacgc tcggcccgat ccgcggtcca cgtggcgggc ctcgtctcgc
     6661 gctacgcgcg ggatcgcctg cgctaccgtc gcggcacgcg gatcgctaac gggaacgccc
     6721 tgatcgcggc actcgcgctc tcggcagagg aaaagagcat cccgctctgg ctctcttcgc
     6781 cacttgtccg gctcgtcata gagaaggggc gcgttgtcgg cggtgtgatt gcgcgggaag
     6841 gacgcgaggt cgaagttcgg gcgnnnnnnn nnnnnnnnnn cggccgagga catgcgcaag
     6901 cggctctatc cgcacgtcgc ccgcggcgcg gagcatcgag cactgccccc ggatagcaat
     6961 acgggtgacg gcatcaggct cggactgtcg gccggtggcg gcttcatcga ggatgtccac
     7021 cacgcggctg cctggacgcc ggtctcgctc gtgccgcaag cgggcggcgg ggtcgtgccg
     7081 tttccgcatt tcatcgatcg cggcaagccc ggctacattg ccgtcgaccg gcggggccgc
     7141 cgcttcgtca atgaggcgaa gtcctatcac gtgttcgtgc cagcgatgat cgaggcctgc
     7201 cgccaggatt cggctgtcga gtgctggctc atcacagatc atagaggtat ccgccgctat
     7261 ggtctcggag cggctgcgcc gagcccgatg ccgctcgggc cgcacttgag atccggctac
     7321 atcgtacgcg gcggcacgct acgagagctc gccgaaaggc taggcatcgg tcccgatggc
     7381 cttgctgcca ctgtcgatgc ctacaacgga ccggcccgcg aaggccgtga cgtgcaattc
     7441 ggcaagggcg gagacgccta ccagcgcttc aatggcgctg cggggcacac acctaacccc
     7501 tgcgtcgcgc cgatcgaaca tcctccctac tatgccgtgc gcctcgtgcc gggcgatatc
     7561 ggcacgttcg ccggtctgaa gaccgatccc cagacgcggg tgctcgatgg cgacgggaga
     7621 gcggtcgcgg ggctctacgc ggtcggcaat gatatggcga gtgtcatggg cggaacatac
     7681 ccaggcgccg gcatcacaat cggcccggcg ctgaccttcg gctacatcgc gggcaggacg
     7741 attgcagccg atgcggccgg gaggagctga gcgtctcgac gaaatgaaaa gggaccggca
     7801 tgacgagccg atccctgatc tctggccggc agcctcgcac cgcggccggc atggctcacg
     7861 cgtcaaagac ttggcggatg cttctgccag tgttgatcgt cgtcacggca tcctggtggg
     7921 caacttcctg gcgggcggcg attgccctcg gcgaattggc cggtcggatc gaagagggac
     7981 agggtggatg gtcgcatgag cttcggcatg ggtgcgctcc tgtcttgcgc ggccaacacg
     8041 tgagccaacc ggccggcacc atgatggagc cggagcacgt cgacgtcgcg ttggcaggat
     8101 ggtggacggg ttcgccgata cgctgcaacg tacgcgaacc aagttggcag gagagcggcc
     8161 ccgtccacgt ccgcttccgc caggcgctgc ctgcactttt gtgatgctgc agttccgaat
     8221 gccgtcacgg ggaccccgat tggctcggac gcgcactgat gctcggatta tctccgagac
     8281 ttcaatagaa taagcgattc atggccgatc gccaacatcg tcgtcaaacc aatagtggcg
     8341 gaccggcacg gggatagcct gacgcgactt gagccggtgt cctgacgtac atgtgtttcg
     8401 ccgcgcaaca gtaacgaatt caggggattc cctctgttcg ttcggcaaga aaatgaatat
     8461 gtcctggcac gtcccaacgg ccgcgtcgag aaaccggtgc ggctcgagcc gggagcaccg
     8521 gatcaccgtc cgccctggac catcggccat ggggcgtgcg cgagccggtt cgcccgatcc
     8581 ttcgggttcg ataggtgatt gtttaccatg tgcggctagc gtccggcgtg gtttcgtgtg
     8641 gttcggggga atctcgcatg cctcgctcat cggcgacgtc ggtcgaatcg gccgtcgacc
     8701 aagtcctgcc gttctgcgtc gtggagcggc gcccgcgggt ggcggtgttc ggcaagatca
     8761 ttctgctctc gctggccggt attgccggcg ccgcaccgat cgtcatggtc acggcgcttt
     8821 cggcctcgac gccggatgcc atcctgcgcg ttgccgacaa ccccctcgtc ggcctgcagt
     8881 tcgcgaccgg actgctgttc tggatcctgc tgctcggcat gccgttctgg cgcttcgtcc
     8941 gctcggctgg catcgagcgc aaggtcgcca tcgacgctgc ctgcgtcacc atcgccgagc
     9001 gcagcctgat cggcgcgcgc gaaaaggtcg tcccgctcac ctcctaccgc ggcatcgctc
     9061 accgcgtccg cgcctcgctt tctgggacac gccacgaact cgtcctcgtc ggtccgagcc
     9121 agcgctcgaa tgtggtcatc gccacggcgc cgcgcatgtc ggaaggagat atcgagcgga
     9181 tgtgcaatct gcttggcctc aaacccgtac ccgcacgtga ggtgatcacg agacgttgag
     9241 gcgtgcggcg ctgcggcggc ttttgaaggg ctgcggcaac ctgctacact cccacccggt
     9301 tcatatacat ccgggtcgct caggagcatg aacgccaagc ccgccccaga catcgaggtc
     9361 ctcatccccg cggaggagct ctcgacgcgt gtcgacgcgc tcgcactcga tattgcgcgg
     9421 ctcgagcggg acaaccttct cgtcgtctcg gtgctgaccg ggagcttcgt cttcgcggcc
     9481 gacctcctgc gcgccctcaa tcgttccggg ctctcgcccg aggtcgattt cctgacactc
     9541 tcgagctacc agcgcggcgt gcgctcgctc gggcaggtcg acatcctgcg cgacgtttcc
     9601 gtggacgtgc gcggccgcaa catcctcctc atcgacgaca ttctcgacac cggccgcacg
     9661 ctcgccttcg ccaaggatct gctcacagcg cgcggggccg ccagcattac gacctgcgtg
     9721 ctcctcgaca aggacgtgcc gcgcgtcgtc gccatcgcgg ccgatttcgc agcgttcaaa
     9781 tgtcccgacg tgttcgtcgt cggatacggt atggatctgt cgcatcgcta tcgtgaactg
     9841 ccattcgtcg gcagggtggt cgtcagggcg tgagcccgcg aactggggga actggcgttg
     9901 ttctgcgttg ccgctttcgc gagcggcggg ggccgctcgc gcgtgacggc gtgagggcgg
     9961 cgcatggtct tcccgacgct cgcgttcctc atcttctatt gcctcgtctg gccgctctcc
    10021 tggggcctcg tcgccttgcg gcggcatggc gcgcacaaac tgtcgatcat cgcggcgagc
    10081 tacatcttct acgccttctg gagctggcgg tttgcgttcc tgttgctctc gagcgtgctg
    10141 ctcaactgga gcgtcggccg agcgatcggc tccagcacct ccccgcgact gcggcgcgct
    10201 cacctcgcct tcggcgtatt gatgaacctc gccgtcctcg gtttcttcaa gtattacggc
    10261 tggttcgccg aagagatggg cgtgctgctg cgcctcgccg gcctctcgcg cgacatcccg
    10321 ctcttcgagg tggtgctgcc agtcggcatc tctttcttca cgttccaggg catctcctac
    10381 atcgtcgatc tctatcgcgg cgacctcgac cgggcgcgtc ccctcgtcga tgtgatgttc
    10441 ttcatctcgt tctttcccca cctcgttgcc ggaccgatcg tgcgtgctgc gagcttcctg
    10501 ccgcagctcg aacggccgcc gcgcccggac cgcgtcttcg ttggcctcgg catgatgctg
    10561 atcgtgtggg gcgtgtggaa gaaggcggtc gtcgccaatt ggctcgcggt gttgctggtt
    10621 gacaaggcat tcttcgatcc cgcggcctat ggcgccctcg atctggccgc cgcgatctac
    10681 ggctatgccg ttcagatcta ctgcgacttc tcggcctaca gcgatatcgc gatcggcgtc
    10741 gcagcgctgc tcggctaccg ctttcaacgc aatttcaatc agccctatcg cgccgtgacg
    10801 ctcgccgact tctggcgtcg ctggcacatg tcgctgtcga gttggctgcg cgactatctt
    10861 tacgtgccgc tcggcggcaa caagggtggg aaggtacgca ccttgcgcaa cctgctgctc
    10921 accatgctgc tcggcggcat ctggcatggg gcggcgtgga agttcgtcgc ctgggggttc
    10981 ctccacggcg gcgcgctcgc gatcgagcgg ctcctcggct tccgtccacg cgagccacat
    11041 gagccggcac cgccagcctg gaagcgggtc ctgccgatcc tgatcgtctt ccacttcgtc
    11101 tgctttgcct ggatcttctt ccgcgccgac gacatggcga aggcgctcgc cgtactggca
    11161 ggcctcgcgg attggcagcg tgcgccgacg ctgctgacgc ccctgcccct cgccctcatc
    11221 gccttcggcc tcgcgatcca gttcacgcct gcaaatacga tcgagcggct cgaccgcatc
    11281 taccagcgac tgccgaccat cgtggtgggc gcactcgtcg gcgcggccct gctgctcatc
    11341 gaggcggtcg gaggcgacgg cagcgcgcca ttcatctact tccagttctg aggaggcacg
    11401 agatgtccga ctggctgcct gatcccggcc tcggcacaga ggacctcgag aaggggatgc
    11461 gtgccggcaa ggcgcgtcac gtgatcccga tcgcgctgat cgcgatcgcg atgcttgcca
    11521 cgttcaattc gggcgggctc gtcaagtggt cggagcggct gccctcgcac gaggcgagcc
    11581 tctgggttgc cgagaaggcc gctgcatggc acgagctgat gctggagctc ggacccgccc
    11641 gcgtcttcga ggacgtgaag gtgcgcctca ggatcgagtg acgatgcgct tggagagggc
    11701 gagcgcaccg gcgagaagcg ccggaaggcc ggcgagcggc aggaacagcg ccacaaggag
    11761 cgtgcagcgc atggccacag ctgcggcata gccgcgccag gcggcgtact cgctccgctc
    11821 ctcgggcggc caaagcgctg caaaccagcg tcgcgcgaca ggcagtgcga cgagccccgc
    11881 ggcgatgagt gcgagccagc cccattgcac gatcgagggg acgccgggaa cgagccgcca
    11941 tctgagctcg cccgcgcgac ttcgactcgt catccagccc tcgatggcat cgatgccgac
    12001 atttcccgtg attgcagtcg ccatctcgtc taggaaggcg ccgacgcgtg atggctccgc
    12061 gccgagatcg ccgatgcgct gtgccttcag gatgatacgg tcggcgtcga ctggcgtgac
    12121 ggcaactgcg agcgtgtcgc tctttccggc gagcgccgcc aacatgtcgc cgaccgtcgg
    12181 ccgcgccgca gcgtggtcga gacccggcac ctcgaccttg agccacagcc agttgcggcc
    12241 ggacggctgg cggggcgcgg cactcgcgag caccatgaga ttgacgtcgc ccgtggtcgc
    12301 cgcgccaacg acatccttga tgaggatcgc gcgctcgccg ccgcgctgcg gctggatcac
    12361 gagacgctcg ccgtcgcggc gggccacgat caacgccgtc tgacccctga gcgccgacag
    12421 cgccccggcg atcctgtcgg gatcgacaat gtcggcctgg ctctgcttcg ttgccttgtc
    12481 gatcctggga cggaccggga tcgacgcgag
//
LOCUS       DAORNH010000115        12493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3819_length_12493_cov_2.551294, whole genome shotgun sequence.
ACCESSION   DAORNH010000115 DAORNH010000000
VERSION     DAORNH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3819_length_12493_cov_2.551294"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PK264_12730"
     CDS             <1..146
                     /locus_tag="PK264_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD76779.1"
                     /translation="KKLEHTVPLGHRGADPAEIAAAILWLASDEAAYCTGTVLDVHGG
                     RSL"
     gene            complement(170..1747)
                     /locus_tag="PK264_12735"
     CDS             complement(170..1747)
                     /locus_tag="PK264_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HRD76780.1"
                     /translation="MTEIVLPKVRNAGVKPLFTGPEWNFELLREAYDAIAEVGLGEMG
                     LDIYKNQIEVITSEQMLDAYSSIGMPMMYRHWSFGKRFAQDESAYRKGYRSLAFEIVI
                     NSDPCINYIMEENTMTMQALVMAHAALGHNHFFKNNYLFRQWTRADMILEYLAFAKRF
                     VTECEERFGVPAVESVLDAAHAIMNQGVSRHARARRRPDPGRDRDREIRRREHEEQTY
                     NELWRTLPVYADTKPEEKDKEKPEGSAEERRRLALPEENLLYFLEKNAPKAEGWKREL
                     LRIVRNLSQYFYPQRQTKVMNEGCATFVHYEIMNRLYERGRITDGSMLEFLHMHAAVV
                     NQPGFSDRNFGGINPYALGFAMMRDIQRICENPTDEDYAWFPDFAGNRDAMGTLRTAW
                     ADYRDESFILQYLSPKVMRDFRLFAIRDNAEAPTVRVEAIHDERGYKDVRRRLARNYD
                     ISVIDPDIQVTDADLAGSRRLVLTHYVRNGILLDKEECDRTLMHVARLWGYRVRLVEV
                     DAQSGKRLREHETLPMP"
     gene            complement(1780..3048)
                     /locus_tag="PK264_12740"
     CDS             complement(1780..3048)
                     /locus_tag="PK264_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010916149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HRD76781.1"
                     /translation="MNVIDRRLNPKKKSLGNRQRFVRRAKADIREAVKDAIKKRKVSE
                     VEGSEQIRIRSKGVGEPTFGHGRDSGIRDAVLPGNKDFNVGDVIPKPQGGGGGRGGKA
                     SADGEGDDDFVFTLSKDEFLDLFFEDLKLPNLVKTKLKDVKSFVPMRAGYTSDGPPAK
                     LNRVRTMRRSIARRIALKRPKTEDVEELERLLAAARAEVPQDEEKIRSLETRLRHIQQ
                     LRRVIAYVDPLDLQYNRTEKRPKPATQAVMFCLMDASASMTEPLKDLAKRFFMLLHVF
                     LSRHYKEVDIVFVRHTSTASEVDEDTFFRGVETGGTVISTALEEMDRIVKDRYSPADW
                     NIYAAQASDGDNIPDDMGHCMELLTEKLLPACQYYAYIEVSERPGPGETVVWQGYKDV
                     AEAAEHFAMRRVSNPGEIFPVFHDLFSEQG"
     gene            complement(3159..5105)
                     /locus_tag="PK264_12745"
     CDS             complement(3159..5105)
                     /locus_tag="PK264_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HRD76782.1"
                     /translation="MATQGEIFDIYSKAYDRQKQVVMPIREYLEGCKKDPTMYSTPAE
                     RMLTAIGEPQIVNTATDQRLGRIFMNRTIKVYPAFADFYGLEETIERIVGYFRHAAQG
                     LEERKQILYLLGPVGGGKSSLAERLKDLMEKCPIYVLKAGDEVSPIFESPLGLFDPDT
                     MGPTLEEKYGIPRRILPGFISPWAVKRLDEFGGDISKFSVVKLYPSKLRQICIAKTEP
                     GDENNQDISALVGKVDIRKLEHFGQNDADAYSYSGGLNRTTQGLLEFVEMFKAPLKML
                     HPLLTATQDRTYVGTENVGAMPYHGIIVAHSNESEWQSFRSNRTNEAFLDRICVIKVP
                     YCLRVTEERRIYDKLLKASELANSPCAPGTLEMLAQFSVLSRLKDHENSQLFSKMRVY
                     DGESLKDTDPQARTLQEYKDAAGVTEGMDGASTRFAYKILSETFNYDSHEIGADPVHL
                     MFVLEQAIKREQLGEDTESRYVEFIKEELAPRYAEFIGNEIQKAYLESYHDYGQNLFD
                     RYVAYADAWIEDHDFKDPDTGQLMNRELLDQELSKIEKPAGIANPKDFRFEVVKFSLR
                     ARARNGGKNPDWTSYEKIRDVIERRMFSQVEDLLPVINFGAKKDGETEKKHHEFVERM
                     VARKYTQRQVRRLVEWYMRVKKAS"
     gene            complement(5641..7452)
                     /locus_tag="PK264_12750"
     CDS             complement(5641..7452)
                     /locus_tag="PK264_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRD76783.1"
                     /translation="MPPVINPNPFTTRPEIDGTFGTVATTHWIGTAVAMGILERGGNA
                     FDAGVAAAFTLQVVEPHLCGPGGDVPVMIVDTRAGGRPEVICGQGPAPKGATIAHYRD
                     RLGLDIIPGTGLLPACIPGTFETFMMVLRDHGTMRLRDVLEAAIGYAEHGHPLVERAN
                     ATIATVADLFRKHWPTSAAVYLPGGQPAPTGKLFTNPQHAATYRRILNEAEAGGGTRE
                     QEIDRARRAWSQGFVAEAIGEFCRSNEVFDVSGRCHKGVLTADDMAGWLPTREAPVGL
                     DYGSYRVLKPGPWTQGPVMLQILALLKGTDFASLAPEGPDFIHLWIEATKLAFADREA
                     FYGDPKFVEVPMESLLSEAYNAERRRLIGPSASLEQRPGRIAGFGGKVIVKATGDKRV
                     SVSSAGAGEPTVGRLGEARGDTVHIDVIDRQGNMFTATPSGGWLQSSPVIPALGFPLG
                     SRGQMFWLEEGQPASLAPGKRPRSTLSVGIALRDGKPYMSWGSPGGDQQDQWNSLLFL
                     RHVHFGMNLQEAIDAPTWHIEHFPASFWPRAARPGVAVLEGRVPKEAIESLRARGHKI
                     EVGADWSEGRLTAASQDGPRRRAAANPRGMQGYAAGR"
     gene            7641..7714
                     /locus_tag="PK264_12755"
     tRNA            7641..7714
                     /locus_tag="PK264_12755"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7673..7675,aa:Gln,seq:ctg)
     gene            complement(7737..>8383)
                     /locus_tag="PK264_12760"
     CDS             complement(7737..>8383)
                     /locus_tag="PK264_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76784.1"
                     /translation="EKVIPWREVQRVEVRDHTFSTGRARVTLKAATVVFVSQVYYDKV
                     LYIGSFFSRGPYWSDKFVDDGTSRGIVIAPEFLSVSAAELSEAITLRWKGSAGGSNWS
                     ITDLGEQSAASGRPVPAHAGSPTGSALPVDGVETLKLALPMVVMAAVAANAVGLWETS
                     GQARDRQRREAWAAEERQRAEERRMHDEKWKTFWDDFDKSMSRIGSGKPEPPQK"
     assembly_gap    8384..8393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8394..8647)
                     /locus_tag="PK264_12765"
     CDS             complement(<8394..8647)
                     /locus_tag="PK264_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76785.1"
                     /translation="MHPDVRNTARPLAYSGARYLRRALMVPLWMLAICVVVIRFASGK
                     AAFAGYIGIVASLVAMAYYAYEIWGRPRPLLQLTSQGMVYR"
     gene            8741..9817
                     /locus_tag="PK264_12770"
     CDS             8741..9817
                     /locus_tag="PK264_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRD76786.1"
                     /translation="MPTVTGRRHFFRLPIEPRRRFIAGNAHVNTLQVLPMAFPPKEKL
                     NVLFAHVAYQFAEPFAARKSGIACEIATSAAELEAKIGSADVLSISGLWRNELIALAP
                     RLKLIQSISAGTDQYGKEQLKAAGIRLASAHGVNANAVAEHAISLVLSLSRQLHLLRD
                     KQAKREWRPMQGNRSIREDEVQGKTVLIVGMGRIGARLAELCKAFGMTVLATKRNPAT
                     GKGAADELHANSSLHTLLPRADYVVLTCPLTPETEKLIGARELAAMKPSASLVNVARG
                     KVCDEPALIAALEKKQIAGAGIDVTVEEPLPAASPLWAMPHVIVTPHTGGETRRYEDR
                     VIDLLLENVARLGRGDAALVNQIV"
     gene            9892..10662
                     /locus_tag="PK264_12775"
     CDS             9892..10662
                     /locus_tag="PK264_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76787.1"
                     /translation="MTAVAQRRASVSEGSLAAFGIDAAEAAALQDAVAFLSPSPLIFE
                     PTGVFDYGRMHEIPEAAFDLYRRSARLSLSHWEQEAIRVSLNRPDGALRHLLDAKASR
                     EGVTDRRTTYLRVDCFGKRIQSPCLLEIWPGGHYSPIHEHAESVGLMKGVFGRVDIML
                     YEGLSSDPKASALLNVTPAHCSWMSPAHFQAHKVYCPMDAHDFAATFHIYTETKSDVF
                     KYREERAPHRIAEFVTESDISWGQFYKALTEAVPYYEA"
     gene            10702..>12493
                     /locus_tag="PK264_12780"
     CDS             10702..>12493
                     /locus_tag="PK264_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76788.1"
                     /translation="MAKIAVVGAGAAGMAAAITLVKAGVDVALFEAMPRLGGECHAAT
                     VALWDGRSIRVDAGISEMNPAMSPALAGLIADLDLRLEPVNADVAFATPDRTPLWFTR
                     VGKPLFRHRPEDANKLLAEIGRFHETSVEVLSDATFASWTAAHYIGQRGFSDDFRKLY
                     LEPRARSLLSVRDAALELLPIRALIATWQMQGIVGHASEPRMTITGGMHAYADAVERL
                     LKSRGVAVHLSTRVAGMTRLGDSVRLRVTGPEGATRNHAFDHVIVAIGAEHVIGILED
                     AQEDEVRALAPVETQRLRQVVHFDNRVLPADRATWGAWNFTTQQGEAHPTSTVYVNRH
                     QNLSPSVPDVFVSINPGIEPEPGKIVLDRWLEHPVIGATSLDNLTRLDLLQGRRRAWL
                     AGGYLREPFSHEQAYQSGIDIAERVLTAIADAARRFEAGLPVSQGGFDTFLREVPMLA
                     HLDPDALAELQLSARPLQAEAGRVLFKQGDAADGLYLVKRGAIDITRRVPGNRAVAIA
                     RRGSGSIVGEMSLLDDSRRSANAIAAEPTSGYFVSAEAFAALRADLRSPAVAVMNAFR
                     REVAARARAAIEEVVARAAVEAVVLRPAVEG"
BASE COUNT         2027 a         4088 c         4114 g         2254 t
ORIGIN      
        1 tgaagaagct cgagcacacg gtgccgctcg gccaccgtgg cgccgatccg gcggagatcg
       61 cggcagcgat cctctggctc gcctcggacg aggccgcgta ctgcacggga accgtgctcg
      121 acgtgcacgg cgggcgcagc ctctgagcga tcgccgagct gtcgcgacgt cacggcatcg
      181 gcagcgtctc gtgctcgcgg aggcgctttc cggattgcgc gtcgacctcg acgaggcgca
      241 cgcggtagcc ccagagccgt gcgacgtgca tcagagtgcg gtcgcactcc tccttgtcga
      301 ggaggatgcc gttgcgcacg tagtgggtga ggaccaggcg gcggctcccc gcgagatcgg
      361 cgtcggtgac ctggatgtcg ggatcgatca ccgagatgtc gtagttgcgc gcgaggcggc
      421 ggcgcacgtc cttgtagccg cgctcgtcgt ggatcgcctc gacacggacg gtcggcgcct
      481 cggcgttgtc acggatggcg aatagacgga aatcgcgcat caccttgggc gagaggtact
      541 ggaggatgaa gctttcgtcg cggtagtcgg cccaggcggt gcgtaacgtc cccatggcat
      601 cacggttgcc cgcgaagtcg gggaaccagg cgtagtcctc gtcggtcggg ttctcgcaga
      661 tgcgctggat gtcgcgcatc atggcgaagc cgagcgcata cgggttgatg ccgccgaagt
      721 tgcgatcgga gaagcccggc tgattcacca cggcggcgtg catatgcagg aactcgagca
      781 tcgagccgtc cgtgatgcga ccgcgctcgt agagccggtt catgatctcg tagtggacaa
      841 acgtcgcgca gccctcgttc atcaccttgg tctggcgctg cgggtagaag tactgtgaga
      901 ggttgcgcac gatgcgcagg agctcacgct tccagccctc ggccttggga gcgttcttct
      961 cgaggaagta gaggagattc tcctcaggca acgcaagccg gcggcgctcc tcggcagagc
     1021 cctccggttt ttccttgtcc ttctcctcag gcttggtgtc ggcatagacg ggtagcgtgc
     1081 gccagagctc gttgtaggtc tgctcctcgt gctcgcgccg gcggatctca cggtcccggt
     1141 cacggccggg atcagggcgg cggcgcgcgc gcgcatgccg gctgacgccc tggttcatga
     1201 tggcgtgggc ggcatcgagc acgctctcga cggcgggcac accgaagcgc tcctcgcact
     1261 cggtgacgaa gcgcttggcg aaggccaagt actcgaggat catgtccgcc cgggtccact
     1321 ggcggaacag gtagttgttc ttgaagaagt ggttgtggcc gagggctgcg tgcgccatca
     1381 cgagagcctg catcgtcatg gtgttctctt ccatgatgta gttgatgcag ggatcggaat
     1441 tgatgacgat ctcgaaggcg agcgagcgat agcccttgcg ataggcgctc tcatcctggg
     1501 cgaagcgctt cccgaacgac cagtgccggt acatcatcgg catgccgatc gaggagtagg
     1561 catcgagcat ctgctcggag gtgatgacct cgatctggtt cttgtagatg tcgaggccca
     1621 tctcgccgag gccgacctct gcgatggcat cataggcctc gcgcagcagt tcgaaattcc
     1681 attcggggcc ggtgaacagg ggcttcaccc ccgcgttgcg aaccttcggc agcacaatct
     1741 cggtcatggg gacctcgcgc ctttctctgt gacccgcggt cagccctgct cggagaagag
     1801 atcgtggaac acggggaaga tctcgcccgg attcgagacg cgccgcatgg caaaatgctc
     1861 ggccgcctcg gcgacgtcct tgtagccctg ccagacgacc gtctcaccgg gtcccggccg
     1921 ctcggacact tcgatatagg cgtagtactg gcaggccggc agaagcttct cggtcaggag
     1981 ctccatgcag tgacccatgt cgtcggggat gttgtcaccg tccgacgctt gcgcggcgta
     2041 gatgttccag tcggcgggcg aatagcggtc cttgacgatc cggtccatct cctcgagcgc
     2101 ggtcgagatc accgttccgc ccgtctcgac gccacggaag aaggtatcct cgtccacctc
     2161 gctcgccgtc gacgtgtgac gcacgaagac gatgtcgacc tccttgtagt gccgcgacag
     2221 gaagacgtgg agcagcatga agaagcgctt ggcgagatcc ttcaagggct ccgtcatcga
     2281 ggcggaggcg tccatgaggc agaacatgac cgcctgcgtt gcaggcttcg ggcgcttctc
     2341 cgtgcgatta tactgtagat cgagcgggtc gacgtaggcg atcactcggc ggagctgctg
     2401 gatgtgccgg agccgcgttt cgagcgatct gatcttttcc tcgtcctgcg gcacctccgc
     2461 gcgagccgcc gcgagaagcc gctcgagctc ctcgacgtcc tcggtcttcg ggcgcttcaa
     2521 agcgatgcgg cgcgcgatcg agcggcgcat cgttcgcacg cgattgagct ttgccggcgg
     2581 cccatcgctc gtatagcccg cacgcatcgg cacgaacgac ttcacgtcct tgagcttggt
     2641 cttgacgagg ttcgggagct tcaggtcctc gaagaagaga tcgaggaatt cgtccttcga
     2701 gagcgtgaag acgaaatcgt catcgccctc gccgtcggcg cttgccttgc cgccgcggcc
     2761 gcctccgccg ccctgcggtt tcggaatgac gtcgccgacg ttgaaatcct tgttgccggg
     2821 aaggacggca tcgcggatgc cgctgtcgcg gccatggccg aaagtcggct cgccgacgcc
     2881 tttcgaacgg atgcggatct gctcggagcc ttcgacctcg gacaccttgc gtttcttgat
     2941 cgcgtccttc acggcctcgc ggatatcggc cttggcacgg cgcacaaagc gctgccggtt
     3001 cccgagactc ttcttcttcg ggttgagccg gcggtcgatg acgttcatgg acacgggagg
     3061 cacactccgg gctcgagcca ccatagcctg tgcatgacgc gacctgctcc gagggcggtg
     3121 cccgcgatct cacgcgagca cgccccggac acactcgctc agctcgcctt cttcacgcgc
     3181 atgtaccatt cgacgaggcg gcgcacctgg cgctgcgtgt acttgcgggc gaccatgcgc
     3241 tccacgaact cgtggtgctt cttctcggtc tcgccgtcct tcttggcgcc gaagttgatg
     3301 accggcagca gatcctcgac ctgggagaac atgcgccgct cgatgacgtc gcggatcttc
     3361 tcgtagctcg tccagtcggg attcttgccg ccgttcctgg ctcgcgcccg gagcgagaac
     3421 ttcacgacct cgaaccggaa gtccttcggg ttggcgatgc cggcgggctt ctcaatcttc
     3481 gagagctcct ggtcgagcag ctcgcggttc atcagctggc cggtgtccgg gtccttgaag
     3541 tcgtggtcct cgatccaggc gtcagcgtag gcgacgtaac gatcgaacag gttctggccg
     3601 tagtcgtgat agctctcgag gtaggccttc tggatctcgt tgccgatgaa ctcggcatag
     3661 cggggcgcca gctcctcctt gatgaactcg acgtagcggc tctccgtgtc ctcgccgagc
     3721 tgctcgcgct tgatcgcctg ctcgagcacg aacatcaggt gcacggggtc tgcaccgatc
     3781 tcgtggctgt cgtagttgaa ggtttccgag aggatcttgt aggcaaatcg tgtcgaggcg
     3841 ccgtccatgc cctcggtgac gccggccgcg tccttgtact cctgcagcgt gcgcgcctgc
     3901 ggatcggtgt ccttgaggct ctcgccgtca tagacgcgca tcttcgagaa gagttgcgag
     3961 ttctcgtggt ccttgaggcg cgaaagcacc gagaactgcg cgagcatctc gagcgtgccc
     4021 ggcgcgcagg gtgaattggc gagctcgctc gccttgagga gcttgtcgta gatgcgcctc
     4081 tcctccgtga cgcggaggca atagggcacc ttgatcacgc agatgcggtc gaggaaggcc
     4141 tcgttcgtcc ggttcgagcg gaagctctgc cactcactct cgttcgagtg ggcgacgatg
     4201 atgccgtgat agggcattgc gccgacgttc tcggtaccga cgtaggtgcg gtcctgcgtc
     4261 gccgtgagca gcgggtgcag catcttcagc ggcgccttga acatctcgac gaactcgagc
     4321 aggccctgcg tcgtccggtt gaggccgccg gagtaggagt aggcatcggc atcgttctgg
     4381 ccgaagtgct cgagcttccg gatgtcgacc ttaccgacga gggcagagat gtcctggttg
     4441 ttctcgtcgc cgggctcggt cttggcgatg cagatctggc ggagtttcga ggggtagagc
     4501 ttgacgaccg agaacttcga gatgtcgccg ccgaactcgt cgagtcgctt caccgcccag
     4561 ggcgaaatga agcccggcag gatacgacgc gggatgccgt acttctcctc gagcgttggc
     4621 cccatcgtgt ccggatcgaa gaggccgagc gggctctcga agatcgggct cacttcgtcg
     4681 cccgccttga ggacgtagat cgggcacttc tccatgaggt ccttgaggcg ctcggcgagc
     4741 gaggacttgc cgccgccgac ggggccgagg aggtagagga tctgcttcct ctcctcgagg
     4801 ccttgcgccg catgacggaa atagccgacg atccgctcga tggtctcctc gaggccgtag
     4861 aagtcggcga aggccgggta gaccttgatc gtgcggttca tgaagatgcg gccgaggcgc
     4921 tggtcggtcg cggtgttgac gatctgcggc tcgccgatgg cggtgagcat gcgctccgcc
     4981 ggggtcgagt acatcgtcgg gtccttcttg cacccttcga ggtactcccg aatcggcatc
     5041 accacctgct tctggcggtc gtacgccttg gagtagatgt cgaagatctc gccttgcgtc
     5101 gccatgtctg tgccctccat ttgcttgggc gatttggctg gtactgacgg accgatccct
     5161 ccagaccggt cattcggatt ttggtcgagc gctacctctg atctcagcgc gaagtgtacc
     5221 tcgaaaacgg gtgaatttga tggcggaacg accagcaggc cgatcaaatc tcgggcaatt
     5281 cggtcggagc atggttcatc tgccacacgg accgggtccg gagggcgagc cctgcccttg
     5341 cctcggccgg gttctgatcc gcgacggagg ctctcggata ttttctcttc ctctcagtgc
     5401 gttgtgacgc cgacttcata ttcatgcctc acttcgcgcg acgctcacgc tcgcgcatcc
     5461 tgcgcaccgg ctcgaagtgc ggggaaaaat aatgacgctc gcgtgttgcc cgcatgcgtc
     5521 atccggcctg ccacgccacg ggcgagggtg tgcccgtgat tcgagcgcac ccgcatgagg
     5581 cgggcgacac tttgaaagtg gccgcgcgag acgcgttttc aagaggccgc ctcagcgaga
     5641 tcagcgaccc gccgcatagc cctgcatgcc acgcggattg gcggcggcac gacgcctcgg
     5701 gccatcctgc gaggcagccg tcaggcggcc ctcggaccag tcggcgccga cctcaatctt
     5761 atggccgcgc gcccgcagcg actcgattgc ctctttcggt acacgcccct cgagaacggc
     5821 gacacccgga cgggccgcgc gtggccagaa cgaggccggg aaatgctcga tatgccaggt
     5881 gggcgcgtca atcgcctcct gcaggttcat gccgaaatga acgtggcgga ggaacaggag
     5941 gctgttccac tggtcctgct ggtctccacc aggcgaaccc cagctcatgt agggcttgcc
     6001 atcgcgcaac gcgatgccga ccgagagcgt cgagcgcggt cgcttgccgg gggcgagcga
     6061 ggctggctgg ccttcctcga gccagaacat ctggcctctc gagccgagcg ggaagccgag
     6121 cgccgggatc accggcgagg actgtagcca gccgcccgac ggcgtcgccg tgaacatgtt
     6181 gccctggcga tcgatgacat cgatatggac ggtatcgccg cgcgcttctc cgaggcggcc
     6241 gacagtcggc tctccggcgc cagcggacga tacagagacc cgcttgtcgc cggtcgcctt
     6301 gacgatcact ttgccgccga agcccgcgat ccgccccggc cgctgctcga gcgaggcgga
     6361 ggggccgatg agccggcgtc gctcggcatt gtaggcctcc gagagcagcg actccatcgg
     6421 cacctcgacg aatttcgggt cgccgtaaaa ggcctcgcgg tcggcgaagg cgagtttcgt
     6481 cgcctcgatc cagaggtgaa tgaagtccgg gccttccggg gcgaggcttg cgaaatccgt
     6541 tcctttgagg agcgccagga tctggagcat caccgggccc tgcgtccacg gtccgggttt
     6601 caacacgcga tagctgccgt agtcgaggcc gaccggggcc tcgcgagtcg gcagccagcc
     6661 cgccatgtcg tcggccgtca gcacgccctt gtggcaacgg cccgagacgt cgaacacctc
     6721 gttcgagcgg cagaactcgc cgatcgcctc cgcgacgaag ccttggctcc aggcgcgccg
     6781 ggcgcggtcg atctcctgct cgcgcgtgcc gccgccggcc tcggcttcat tgagaatgcg
     6841 cctgtaggtc gccgcgtgct gcgggttcgt gaatagcttg ccagtcggcg ccggttggcc
     6901 acccggcaga tagacggcgg ccgacgtcgg ccaatgcttg cggaaaaggt cggcgacggt
     6961 tgcgatggtg gcattggcgc gctcgacgag ggggtggccg tgctccgcgt agccgatcgc
     7021 cgcctcgagc acatcacgga gccgcatggt gccatggtcg cgcaacacca tcatgaaggt
     7081 ctcgaatgtg cccgggatgc aggccggcag cagccccgtt cccgggatga tgtcgaggcc
     7141 gaggcggtcg cggtagtgtg caatcgtggc gcccttgggc gccggaccct ggccgcagat
     7201 aacctcgggc cggccgccgg cgcgcgtgtc gacgatcatc accgggacgt cgccgcccgg
     7261 accgcagaga tgcggctcga cgacctgcag cgtgaaggcg gcagcgaccc cagcatcgaa
     7321 ggcattgccg ccgcgctcca ggatacccat tgcaaccgcg gtgccgatcc agtgcgtcgt
     7381 cgcgacggtg ccgaatgtgc cgtcgatctc gggtcgcgtc gtgaaggggt tcggattgat
     7441 gaccggcggc atggcgggct ccgagagaaa gtggcgtctt cccgagggct agcatgcgtg
     7501 cggccggacc gacaggcccg atcgctatcg ggctcatcgc tgctggccgg ccgacgtccc
     7561 atgacgacgg tttctgattt ggacacggtc cttgcgcggg cgccgttcga ttgctagata
     7621 gcgcgcctgc gatccggtca tgggggatcg tctaatggta ggactgcaga ctctgactct
     7681 gcctgtctag gttcgaatcc tagtccccca gccaaccgca gcgcgggcag cgcctttcat
     7741 ttctgtggcg gctccggctt gcccgaccct atgcggctca tgctcttgtc gaagtcgtcc
     7801 cagaatgtct tccatttctc gtcgtgcatg cggcgctctt cggcgcgctg acgctcctcg
     7861 gcggcccatg cctcgcgcct ctggcggtcg cgcgcctgac ccgatgtctc ccagaggccg
     7921 acggcattcg cggcgaccgc ggccatgacg accatgggga gagcgagctt gagggtctcc
     7981 acgccatcga cgggcagagc cgaacctgtc ggggaccctg catgggccgg cactgggcga
     8041 ccggatgcgg cgctctgctc tccgaggtct gtgatcgacc aattcgaacc gccggcggaa
     8101 cccttccagc gcagtgtgat agcttcgcta agttcggcgg cgctgaccga caagaactcg
     8161 ggcgcgatca cgatgcccct ggatgtgccg tcatcgacga acttgtcgct ccaatagggc
     8221 ccgcgcgaga agaacgagcc gatatagagc accttgtcgt agtagacctg cgacacgaac
     8281 acgacggttg cggccttgag cgtcacgcgc gcacgtcccg tcgagaaggt gtgatcgcgc
     8341 acctcgacgc gttgcacctc ccgccagggt atgaccttct cggnnnnnnn nnncgataga
     8401 ccatgccctg cgacgtgagc tgcaggagcg gtctcggccg cccccagatc tcgtaggcgt
     8461 agtaggccat ggcgaccagt gaggcgacaa tgccgatgta gcctgcgaac gcggccttgc
     8521 cgctggcgaa gcggataacc acgacgcaga tggccagcat ccagagcggc accataaggg
     8581 cgcgcctcag atagcgcgca cccgagtacg cgagcgggcg cgccgtgttc ctgacgtcgg
     8641 ggtgcatcaa actgcctcgt cgccatcgac ctgcttcgat cgatccatac aagtccgccc
     8701 cccgtgagcc tggcaaggcc tacctcgagc catgcttgcc atgcccaccg tgacgggtcg
     8761 ccggcacttt ttccgcttgc cgatcgagcc gcggcgacgc ttcattgcgg gcaacgccca
     8821 cgtcaacacc ctccaggtgc tccccatggc cttcccgccc aaagaaaagc tcaacgtcct
     8881 tttcgcccac gtcgcctacc agttcgccga gccgttcgcg gcgcgcaagt cgggcatcgc
     8941 ctgtgagatc gcgaccagcg ccgccgagct cgaggcgaag atcggaagcg ccgatgtgct
     9001 ttcgatctcc gggctctgga ggaacgagct gatagccttg gcgccgcgcc tgaagctcat
     9061 ccagtcgatc agcgccggca ccgaccagta cggcaaggag cagctgaagg cggctggcat
     9121 ccgcctcgcc tcggcccatg gcgtcaacgc caacgccgtc gccgagcacg cgatcagcct
     9181 ggttctatca ctgtcgcgcc agctgcacct ccttcgcgac aagcaggcaa agcgcgagtg
     9241 gcgccccatg caaggcaacc gcagcattcg agaggacgag gtgcagggca agaccgtgct
     9301 aatcgtcggc atggggcgca tcggcgcgcg gctcgctgaa ctgtgcaagg cgttcggcat
     9361 gacggtgctg gcgacgaagc gcaatccggc aactggcaag ggcgccgcag acgagttgca
     9421 cgccaacagt tccctgcata cgcttctgcc tcgcgccgac tacgtcgtcc tgacctgccc
     9481 gctgaccccc gaaaccgaaa agctgatcgg cgcgcgcgag cttgccgcga tgaagccctc
     9541 ggcctccctc gtcaacgtcg cccgcggcaa ggtctgcgac gagccggcat tgattgcggc
     9601 cctcgaaaag aagcagatcg ctggtgccgg catcgacgtg acggtcgagg agccgctgcc
     9661 ggcggcgtcc ccgctctggg ccatgccgca cgtgatcgtc accccgcaca cgggcggcga
     9721 gacgcggcgc tatgaggacc gcgtgatcga cctcctcctc gagaacgtgg cgcggctcgg
     9781 ccgcggcgat gcggccctcg tcaaccagat cgtgtgatca ccggcacagg ttggcgcgat
     9841 cgacagcatt atgccggtgg ccacaaaccg gatcgagcgc gggatggcga tgtgacagca
     9901 gtagcccaac gacgagcgag cgtctcggaa ggaagtcttg ccgcattcgg catcgacgcc
     9961 gccgaggcgg cggcgctgca ggatgcggtc gcatttctct cgccctcgcc gctgatcttc
    10021 gagccgacgg gcgtcttcga ctacggccgc atgcacgaga tcccggaagc ggccttcgac
    10081 ctctaccgcc gctcggcgcg cctctcactc tcgcactggg agcaggaggc gatccgcgtg
    10141 agcctcaatc gacctgacgg ggcacttcgc cacctgctcg acgccaaggc atctcgcgag
    10201 ggcgtcaccg accggcgcac gacctatctg cgcgtcgatt gctttggcaa gcgcatccag
    10261 tctccgtgcc tgctcgagat ctggccgggc gggcactact cgccgatcca cgagcacgcc
    10321 gagagcgtcg gcctgatgaa gggtgtgttc ggccgggtcg acatcatgct ctacgagggc
    10381 ctctcgagcg atccgaaggc gtcggcgctg ctcaacgtca cgccggcgca ttgctcctgg
    10441 atgtcgcccg cacacttcca ggcgcacaag gtctattgcc cgatggacgc gcacgacttt
    10501 gccgcgactt tccacatcta cacggagacg aagagcgatg tgttcaagta tcgcgaggag
    10561 cgcgcgccgc accgcatcgc cgagttcgtg acggaatccg acatcagctg gggccagttc
    10621 tacaaggcgc tgaccgaggc cgtgccctac tacgaggcct gaggcagcgt gagccgcgac
    10681 aggaaccgag gagacgtgga gttggccaag attgccgtcg tgggtgccgg ggccgccgga
    10741 atggccgccg ccatcacgct cgtaaaggcc ggtgtcgatg tcgcgctgtt cgaggcgatg
    10801 ccgcggctcg gcggcgaatg tcacgcagcg accgtcgcac tgtgggacgg gcgctccatc
    10861 cgcgtcgatg ccggcatcag cgagatgaac ccggcaatgt cgcctgccct cgcgggtctc
    10921 atcgccgacc tcgatctccg gctcgagccg gtcaatgcgg acgtggcctt cgcgacgccg
    10981 gaccgcacgc ccctctggtt cacgcgtgtc ggcaagccct tgttccgaca ccggcccgag
    11041 gacgccaaca agctgctcgc agaaatcggg cgcttccacg agacctcggt cgaggtgctt
    11101 tcggacgcaa ctttcgcaag ctggacggct gcgcactaca tcgggcagcg cggattttcg
    11161 gatgatttcc gcaagctcta tcttgagccg cgcgctcgct cgctcctctc ggtccgcgat
    11221 gccgccctcg agctcctgcc tatccgggcc ctgatcgcga cctggcagat gcaagggatc
    11281 gtcggtcatg cgagtgagcc gcgcatgacg atcacgggcg gcatgcatgc ctatgccgat
    11341 gccgtcgagc gccttctcaa gtcgcgcggc gtcgccgtcc atctttcgac gcgggtcgcc
    11401 ggcatgactc gcctcggcga cagcgtacgc ctccgcgtca ccgggccgga gggcgcgacc
    11461 cgtaaccacg ccttcgacca cgtcatcgtc gccattggcg ccgagcatgt gattggcatc
    11521 ctggaggacg cgcaggagga cgaggtgcgt gcgctggctc ccgtcgagac gcagcgcctc
    11581 cgccaggtcg tgcatttcga caaccgcgtc ctgccggcgg atcgtgcgac ctggggtgcc
    11641 tggaacttca cgacacagca gggcgaggcg catccgacgt cgacggtata tgtcaaccgc
    11701 caccaaaacc tctcgccctc ggtgccggac gtgttcgtct cgatcaatcc cggtatcgag
    11761 ccggagccgg gaaaaatcgt cctcgatcgc tggctcgagc accccgtcat cggggctacg
    11821 agcctcgaca acctgacgcg cctcgatctc ctgcagggcc ggcgccgcgc ctggctcgcg
    11881 ggcggctacc tgcgcgagcc tttcagccac gagcaggcct accagtcggg gatcgacatc
    11941 gccgagcgcg tcctgacggc gattgcggat gccgcgcgcc ggttcgaggc cggcctcccg
    12001 gtgagccagg gcggcttcga cacattcttg cgcgaggtgc cgatgctcgc ccacctagat
    12061 cccgatgcgc ttgccgagct gcagctttcc gcgcggccgc tccaggcgga ggcaggacgg
    12121 gtgcttttca agcagggtga cgcggcggac ggcctctatc tcgtgaagcg cggcgccatc
    12181 gacatcacgc ggcgcgtgcc gggcaatcgc gccgtcgcca tcgcgcgtcg gggctccgga
    12241 tcgatcgtcg gcgagatgag cctgctcgac gactcgcgcc ggtcggccaa cgcgatcgcg
    12301 gccgagccga ccagcggcta cttcgtctcc gccgaggcct tcgcggcgct gcgggcggac
    12361 ttgcggtcac ccgcggtcgc cgtcatgaac gccttcaggc gcgaggtcgc cgcacgtgcc
    12421 cgcgccgcca tcgaggaggt cgtcgcccgc gccgccgtcg aggctgtcgt cctgcgtccc
    12481 gccgtcgagg gtg
//
LOCUS       DAORNH010000116        12455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3835_length_12455_cov_2.543710, whole genome shotgun sequence.
ACCESSION   DAORNH010000116 DAORNH010000000
VERSION     DAORNH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3835_length_12455_cov_2.543710"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PK264_12785"
     CDS             <1..887
                     /locus_tag="PK264_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRD76789.1"
                     /translation="PITFGHKCAIWLDEMLRHLERLSALEARVFVGSLVGAVGSQAAL
                     GPKAREIETRLMQRLGLDVPHISFAPARDRFVEYATVLGLFGGTLAKIANEMFNLGRN
                     EVGEVEELFTAGKIGSSTMPHKRNPVIAENLIGLGRALRHNVAMMLEAMVQEGERDAA
                     SWKTEWKALPEACMITGAMLAQTKRLLEGVKVDEAAMRRNLDILAGYLLSERVMLALG
                     ERVGKQTAHQWVYEASMRGIEDQLPFAAALRGHARIRDAFDDDEITRLTDPATYTGEC
                     EAAIDRVLVAAKAARVIN"
     gene            927..2528
                     /locus_tag="PK264_12790"
     CDS             927..2528
                     /locus_tag="PK264_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76790.1"
                     /translation="MTLKRSFLTALSVAALGGTIMLPDATPALGQAPKKGGTLKFGVV
                     AEPPTIDCHAATTFAMVHPVAPQYSTLLKFVGRHDSMKIVGDLAESYEMAKDGLTYTF
                     KLRKGVKFHDGTDFTAADIKATYERIARPPEGVVSVRKAVHGDLGDIETPDAHTVVFK
                     MKKVNASMLLHFASPFNCVYSAARLKADPQFPAKTVMGTGAFQFVEYQKGAAWKAKRF
                     DGYFNKDRPYLDGYIAYFIKTPGIVPGLQGGQIDAEFRGRTPKERDTLKAAMGDKISI
                     EEGPWITNIQLVFNTQKKPFDDVRVRKAITHAIDRWGGSENMGKVSLIKGVSGVFRPG
                     APYSLSNAELEKLPGFWKDITKARAEAKKLLAEAGVSDLKFKLLNRQIGEPFVPAGIY
                     FVDQMRIIGVPVEHLLLETKLYFDRLHAGDFDMAVNNVSDFVDDPNAQFNTLLSAKRS
                     SIAYSRHADTKIDELYDRQSGTVDEKARLAIVHELEKYIHETSYSIPLLWYQRIVVNH
                     KKVKGWELHPSHFSGQSLVDVWLDE"
     gene            2598..4196
                     /locus_tag="PK264_12795"
     CDS             2598..4196
                     /locus_tag="PK264_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76791.1"
                     /translation="MKRYVILASAATLAASAAAVVMPGDTLGQGGPKKGGTLNFAVVA
                     EPPTIDCHAVSTFAFAHPGRPHYSTLLKFAGDYKNLKIVGDLAESYEMAKDGLTYTFK
                     LHKGVKFHDGADMTSADIKASYDRIVNPPAGVTSLRKALHADIGSVETPDAHTVVFKM
                     KRANASMLTHFASPWNCIYRAALLKEDPNFPAKKIVGTGPFVHVEYVKGSHWSGKRFD
                     GYFRKDRPYLDGYKALFVKSAAVAPGMVGGQFDAEFRGRTPKERDQIMAGLKDKAVTI
                     EGPWVTNLLFTFNTTKKPFDDERVRRALSMAIDRHGGSEPLSKISMLKYVSGLLRPGF
                     DMGLPPAELEKLPGFSKDINKSREEAKKLLAEAGVPKLKVRLVNRNIDEPYKPGALFV
                     IDQWRQIGVETEHVVLETKLFFDAMKDGNFDVLVEFISDFADDPSAQFDKLLTGQKSS
                     QSASRHTDKRLDELFDLQAAAVDPAERRKLVHEFERHALTKAYSVPLLWWQRIITMHK
                     KVKGWEHQANHFTGQDLGDVWLDQ"
     gene            4262..5269
                     /locus_tag="PK264_12800"
     CDS             4262..5269
                     /locus_tag="PK264_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76792.1"
                     /translation="MLGFIVRRLLLMIPTLVGVAIAVFFMLRLGGGDIVAMKFRAEGA
                     QVTEDVIQAERKRLGLDKPNFYVSCAKYVPPSGCTVSMDNQFADYMGGLLRFDLGNSL
                     WTGDPVIKDIAIRFPVSLQVAIMAMLIAVLIAVPLGTISALYRDTWIDYVVRVFSVSG
                     LAIPSFWLGMLMILGLLHFFQWLPKIGYVSIFEDPVANIAALIWPALSVGYRYAAVAT
                     RMMRSSLLEVMREDYIRTARAKGVWSKLITRRHALRNAMLPVVTVIGLEFAFLIGGLV
                     VTEQVFNLNGIGRLFVDASSRGDFVLIQGLVLMIAVIFIMINLLVDIFYGFLDPRIRL
                     N"
     gene            5299..6198
                     /locus_tag="PK264_12805"
     CDS             5299..6198
                     /locus_tag="PK264_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD76793.1"
                     /translation="MADITASSSTAGAIPKRPNPVMLFIKSQPLGFAGFLIVLLYVVL
                     AVGRAWIATHEPTAMSLGDMEQPPSLKNFFGTDQMGRDVFSRVVYGARTALLIGILSA
                     LFGGTIGALLGATSGYFGGRIDTFLQRIVDIMLSFPIIILAVVVVAVLGKWPVFDVDM
                     NLIIAIALPMIPKMARIARSSTLSIVQMPFIDAARAAGFGHTRIILRHIMPNIIAPYL
                     IMVTAFIAQAILLEASLSFLGLGVTEPTPAWGLMLSGSSADFARTAPWMIIFPGLAIT
                     LAVFAFNLLGDSLRDWLDPKMKG"
     gene            6319..>7114
                     /locus_tag="PK264_12810"
     CDS             6319..>7114
                     /locus_tag="PK264_12810"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD76794.1"
                     /translation="MDFKGKVAVVTGAANGIGRATSFAFAKAGAKVVLVDRDEAGAKA
                     AAGAIVQQGGTAHGVKADVTRSADVQAYVKAALETYGRIDAFFNNAGIEGKVTPIVEY
                     DEEMFDQVIGINVKGVFLGLRYVLPVMLKQGSGAIVNTASVAGIVGTPGIAPYVASKH
                     AVIGLTKSVSGEVARQGVRVNAVCPGPVDTRMIHSLEEQINPADPGSVGQRYKGAIPL
                     GRYATVDEIANMVLFLCSDLASAMTGGHYVVDGGRTATGGAVTNVAM"
     assembly_gap    7115..7165
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7166..>7468
                     /locus_tag="PK264_12815"
     CDS             <7166..>7468
                     /locus_tag="PK264_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HRD76795.1"
                     /translation="NSKKVVVPNTMCKAGIEILRSRPEVTVVEFDNFVSSADFQKFLA
                     AQGGVHAGILGATPWSKGECEAAKTLEVVARIGVGFDAVDVPELTRRKIPLMVVGT"
     assembly_gap    7469..7629
                     /estimated_length=161
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7630..8145
                     /locus_tag="PK264_12820"
     CDS             <7630..8145
                     /locus_tag="PK264_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76796.1"
                     /translation="RIGTRTAKRAAAMEMTVQIYDPYVKPEVIKAAGYEPVKDLDAAL
                     PRADFVSIHCPKNAETKGMFDGKRLALLKPTAYLVNTARGGLIDEIALHKALTTGKLA
                     GAGLDVFDSEPVKPDNPLLKLDNVITAPHMAGVTLESVDRMGQQAARNVLSVFDRAII
                     RENVVNQEILK"
     gene            8323..9375
                     /gene="plsX"
                     /locus_tag="PK264_12825"
     CDS             8323..9375
                     /gene="plsX"
                     /locus_tag="PK264_12825"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRD76797.1"
                     /translation="MPARQTIAIDAMGGDFGPEVTIPGAALAAERHPNATFVFYGNQA
                     RIDAALVQHGALKGRSRVVHTDLVVAMDDKPSQAMRRGKGSSMWLAIEAVQKGEAHAA
                     VSAGNTGALMAMAKLILRTLPGIERPAIAALWPTVNGECVVLDVGANIGASARQLADF
                     ALMGAAMARAIFHIERPTVGLLNVGVEEIKGAEEVKEAHALLKTPSLEPPFTYKGFVE
                     GNEIGQGAADVVVVEGFAGNIALKTAEGTARQIGHWLKSALTQSIISKLGAIMAQGAL
                     RAFKRKMDPGRVNGGTFLGLNGIVIKSHGSADAPSFAGAVDLGYDMAESALLERLATD
                     IDALHGKIETEAQVTA"
     gene            9507..10484
                     /locus_tag="PK264_12830"
     CDS             9507..10484
                     /locus_tag="PK264_12830"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRD76798.1"
                     /translation="MAVIRSVIRGVGAYLPKRVMTNDDISRIVDTDNDWIVERTGIKS
                     RHIAAEGELTSDLGIMASKQALIRAGIDPVDIDLVICATATPDRTFPATAVKIQSALG
                     VTKGAAFDIQAVCSGFVYALATADNFLRTGQFNRALVVGAETFSRILDWSDRGTCVLF
                     GDGAGAVVLEAQPQRGQKTDRGILHSIIRSDGRYEDLLYVDGGPGSTKTVGHLRMNGR
                     EVFRHAVQKISGVIEETLVATGYSAEEIDLFVPHQANKRILDGIAKRLNVAPGKIVMS
                     LDQHGNTSAASIPLALNQAFEAHKVKEGGLIMMEAMGGGFTWGAVLLRW"
     gene            10625..10936
                     /locus_tag="PK264_12835"
     CDS             10625..10936
                     /locus_tag="PK264_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HRD76799.1"
                     /translation="MGHRTLTRADLAEAVVQKVGLPRNESQDLVELVLGEISTSLAEG
                     KAVKLSSFGSFGIRSKGERVGRNPKTGEEVPITPRRVLVFRPSNIMKDRINAGRLRRK
                     P"
     gene            11301..11822
                     /locus_tag="PK264_12840"
     CDS             11301..11822
                     /locus_tag="PK264_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD76800.1"
                     /translation="MARAAVTADKSTDAFRTISEVAESLDVPKHVLRFWELKFTHIRP
                     MKRGGGRRFYRPEDVELLRGIQYLLHGEGYTIRGVQKILRRHGIELVKSCWRDGAAQL
                     RPEGFEREEPGARTQKAGATAAKAGKRRDTALLSEDGGQALIASAIRELTACRELLTG
                     LLRAEGSAKGRPG"
     gene            11826..>12455
                     /locus_tag="PK264_12845"
     CDS             11826..>12455
                     /locus_tag="PK264_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76801.1"
                     /translation="MPAQRQGEVWRVDCEEVARHGLDGARGIDIVPADEIERSRTFAD
                     RDRRRHWLALRIALRSALVPWIGRTDAAGPFRLGAAGRPALNGHGPSFSISHTGPLGL
                     VAIATDSIGVDIEPERAAAIAAWRRSAIEAAAIMLAPGLALPDAPEPRFIQCWVRLEA
                     YAKASGWGIGRTLVELGAAGARQGEPAGGDQPSSPGGPAEASPNWSVVDL"
BASE COUNT         2233 a         3955 c         3944 g         2111 t
ORIGIN      
        1 tgccgatcac cttcggccac aagtgtgcga tctggctcga cgagatgctg cgtcacctcg
       61 agcggctgag cgcactcgag gcgcgcgtct tcgtcggcag cctcgtcggc gcggtcggct
      121 cgcaggcggc cctcggtccc aaggcgcgcg agatcgaaac ccgactcatg cagcggctcg
      181 ggcttgatgt accgcacatc tccttcgccc ccgcgcgcga tcgcttcgtc gagtatgcga
      241 cggtgctcgg cctcttcggc ggcacgctcg cgaagatcgc caacgagatg ttcaacctcg
      301 gccgcaacga ggtcggcgag gtggaggagc tgttcaccgc cggcaagatc ggctcctcga
      361 ccatgccgca caagcgcaac cccgtgatcg ccgaaaatct catcggcctc ggccgtgcgc
      421 tccgccacaa cgtcgcgatg atgctcgagg cgatggtgca ggagggtgag cgcgatgcgg
      481 cgtcctggaa gacggagtgg aaggcgctcc cagaggcctg catgatcaca ggcgccatgc
      541 tcgcgcagac gaagcgcctc ctcgagggcg ttaaggtcga cgaggccgcc atgcgccgca
      601 acctcgacat cctcgcgggc tatctgctgt ccgaacgtgt catgctggcg ctcggcgagc
      661 gcgtcggaaa gcagacggcg caccagtggg tttacgaggc gtccatgcgc ggcatcgagg
      721 accagctgcc gttcgccgcg gcgcttcgcg ggcacgctcg catccgcgat gcattcgacg
      781 acgacgaaat cacgcggctt accgatcccg cgacctatac gggggagtgc gaggcagcga
      841 tcgatcgagt tctggttgca gccaaggccg cccgcgtgat caactgaggt tacgactgaa
      901 aaagaatggc tgaggaggaa agccgaatga cactgaagcg aagtttcctg acggcgctgt
      961 ccgttgccgc cctcggcggc acgatcatgt tgccggacgc gacgccggcg ctcggccagg
     1021 cgccgaagaa gggtggcacg ctcaagttcg gcgttgtcgc cgagccgccg accatcgact
     1081 gtcacgcggc gacgacgttc gccatggtgc accccgttgc cccgcagtac tcgacgctct
     1141 tgaagttcgt cggccgccat gattcgatga agatcgtcgg cgatctcgcc gagagctacg
     1201 agatggcgaa ggacggcctc acctacacct tcaagctccg caagggggtg aagttccacg
     1261 acggcacgga tttcactgcc gccgacatca aggcgaccta cgagcgcatc gcgaggccgc
     1321 ccgagggcgt ggtctcggtc cgcaaggccg tgcacggcga cctcggagat atcgagacgc
     1381 ccgatgcgca caccgtcgtc ttcaagatga agaaggtgaa cgcgtcgatg ctcctgcact
     1441 tcgcgtcgcc cttcaactgc gtctacagcg ccgcccgcct caaggccgac ccgcagttcc
     1501 cggccaagac cgtcatggga accggcgcct tccagttcgt cgaataccag aagggtgcgg
     1561 cctggaaagc caagcgcttc gacggctact tcaacaagga ccggccgtat ctcgacggct
     1621 acatcgccta cttcatcaag acgccgggca tcgtgccggg cttgcaaggc gggcagatcg
     1681 acgccgagtt ccgtggccgc acgcccaagg agcgcgatac gctcaaggcg gccatgggcg
     1741 acaagatctc gatcgaggaa ggcccctgga tcaccaacat ccagctcgtc ttcaatacgc
     1801 agaagaagcc cttcgacgat gttcgcgtgc gaaaggccat tacgcatgcc atcgaccgct
     1861 ggggtggctc ggagaacatg ggcaaggtgt cgctcatcaa gggtgtcagc ggcgtgttcc
     1921 ggccgggtgc gccctactcg ctgtccaatg ccgagctcga gaagctgccc ggcttctgga
     1981 aggacatcac caaggcgcgc gccgaggcga agaagctact ggccgaggcc ggcgtgtcgg
     2041 acctcaaatt caagcttctg aatcgccaga tcggtgagcc gttcgtgccc gccggcatct
     2101 acttcgtcga tcagatgcgc atcatcggcg tgccggtgga gcacctgctg ctcgagacga
     2161 agctctactt cgaccgcctg cacgccggtg acttcgacat ggcggtcaac aatgtgtcgg
     2221 atttcgtcga cgacccgaat gcccagttca atacgctgct gagcgccaag cgctcatcga
     2281 tcgcgtactc gcgtcacgcc gacacgaaga tcgacgaact ctatgatcgc cagtccggta
     2341 cggtggatga gaaggcacgc ctcgcgatcg tgcatgaact tgagaaatac atccacgaga
     2401 catcctacag catcccgctg ctctggtatc agcggatcgt ggtgaaccat aagaaggtga
     2461 agggctggga gctgcatccg agccacttct cgggccaatc gctcgtcgat gtctggctcg
     2521 acgagtgacg acggaatgcg gcggcgccac cgatgccgcc gccgacctca atcgaagttc
     2581 cagggaggac tacagacgtg aaacgctatg tcattctggc atcggccgcg acgctggcag
     2641 cgagtgctgc tgcggtcgtc atgcctggcg ataccctagg ccagggcggc cccaagaagg
     2701 gcggaacgct caacttcgcc gtcgtcgccg agccgccgac catcgactgc catgccgtgt
     2761 cgaccttcgc cttcgcccat ccgggccggc cgcattactc gacgctgctc aagttcgccg
     2821 gcgactacaa gaacctgaaa atcgtcggcg acctcgccga gagttacgag atggccaaag
     2881 acggcctcac ctacaccttc aagctgcaca aaggcgtcaa gtttcacgac ggcgccgaca
     2941 tgacgtcagc agacataaag gcgagctacg accgcatcgt caatccgccg gcgggcgtca
     3001 cctcgctcag gaaggcgctg cacgccgaca tcggctcggt cgagacgccg gacgcgcaca
     3061 ccgtcgtctt caagatgaag cgggcgaatg cgtcgatgct cacgcacttc gcctcgccct
     3121 ggaactgcat ctaccgcgcc gccctcctca aagaggaccc gaatttcccg gcaaagaaaa
     3181 tcgtcggcac cggcccgttc gtgcacgtcg agtacgtgaa gggctcgcac tggtcgggca
     3241 agcgcttcga cggctacttc cgcaaggatc gtccgtatct cgacgggtac aaggcgctgt
     3301 tcgtgaagtc ggcggccgtt gcccccggca tggtcggcgg ccagttcgac gccgaattcc
     3361 gcggtcgcac gccgaaagag cgcgaccaga tcatggccgg cctcaaggac aaggccgtca
     3421 ccatcgaagg tccgtgggtg acgaacctgc tgttcacctt caacacgacc aagaagccct
     3481 tcgacgacga gcgggtgcgc cgcgcgctgt cgatggccat cgaccgccac ggcggctcgg
     3541 agccgctctc caagatctcc atgctgaagt acgtgagcgg gcttctgcgc ccaggcttcg
     3601 acatgggact accgccggct gagctcgaga agctccctgg gttctcgaag gacatcaaca
     3661 agtcgcgcga ggaggcgaag aagctgctcg ccgaagccgg cgtgccgaag ctcaaggtgc
     3721 gtctcgtcaa ccgcaacatc gacgagccct acaagccggg tgcgctgttc gtcatcgacc
     3781 agtggcgcca gatcggcgtc gagaccgagc acgtcgtgct cgagacgaag ctcttcttcg
     3841 acgccatgaa ggacggcaac ttcgacgtgc tcgtcgagtt catctccgac ttcgcggatg
     3901 atccctcggc gcagttcgac aagctgctca cgggccagaa atcgtcgcag tcggcatcgc
     3961 gacacaccga caagcggctc gatgagctgt tcgacctgca ggcggcggcc gttgatccgg
     4021 cagagcggcg caagctcgtt cacgagtttg agcggcacgc attgacgaag gcctactcgg
     4081 tgccgctcct gtggtggcag cgcatcatca ccatgcacaa gaaggtgaag ggctgggagc
     4141 accaggccaa tcacttcacc ggccaggacc tgggtgacgt ctggctcgac cagtgaacgt
     4201 gtcaagaaac atggggcagg ccgccggcct gcccctccgt ccttttcgat aggtttcgaa
     4261 tatgctcggc ttcatcgtcc gccgcctcct cctgatgatc ccgacgctcg tcggtgtcgc
     4321 gatcgccgtg tttttcatgc tgcgtctcgg cggcggcgac attgtcgcca tgaagttccg
     4381 tgccgaaggc gcgcaggtga cggaggacgt catccaggcg gagcggaagc gcctcggcct
     4441 cgacaagccg aacttctacg tttcgtgcgc gaagtacgtg ccgccgtcag gctgcactgt
     4501 ctctatggac aaccagttcg ctgactacat gggcggcctc ttgcgtttcg acctcggcaa
     4561 ctcgctgtgg acgggcgacc cggtgatcaa ggacatcgcc atccgctttc ccgtgtcgct
     4621 gcaggtcgcc atcatggcga tgctgatcgc cgtgctgatc gcggtcccgc tcgggacgat
     4681 ctcggcgctc taccgcgaca cctggatcga ctacgtggtg cgcgtattct ccgtgtccgg
     4741 cctcgcgatc ccctcgttct ggctcggcat gctgatgatc ctcgggctcc tgcacttttt
     4801 tcagtggctc cccaagatcg gctacgtgtc gatcttcgag gatccggtcg ccaacatcgc
     4861 ggcgctcatc tggccggcac tttccgtcgg ctatcgctac gcagctgttg cgacgcgcat
     4921 gatgcgctca tcgctgctcg aggtgatgcg ggaggactat atccgcacgg cacgcgccaa
     4981 gggtgtgtgg tcgaagctca tcacgcgccg acatgcgctg cgcaacgcga tgctgccggt
     5041 cgtcacagtg atcggactcg agttcgcctt cctgatcggc ggtctcgtcg tcaccgagca
     5101 ggtgttcaac ctcaacggca tcggcaggct cttcgtcgat gcctcgagcc gcggcgactt
     5161 cgtgctgatc cagggcctcg tgctgatgat cgctgtgatc ttcatcatga tcaacctgct
     5221 cgtcgatatc ttctacggct tcctcgatcc gcgcatccgc ctcaactgac ccgcgctgac
     5281 ctcaaagagt gacggaccat ggctgacatc acggcatctt ccagcaccgc cggcgcaatt
     5341 ccgaagcgcc cgaacccggt catgctgttc atcaagtcgc agccgctcgg cttcgccggc
     5401 ttcctaatcg tgctcctcta tgtcgtcctg gcggttgggc gcgcctggat cgccacgcac
     5461 gagccaacgg cgatgagcct cggcgacatg gagcagccgc cctcgctcaa gaacttcttc
     5521 ggcaccgacc agatgggccg cgacgtcttc tcgcgcgtcg tctacggagc gcgcacggct
     5581 ctgctgatcg gcatcctgtc ggccctcttc ggcggcacga tcggcgcgct gctcggcgcg
     5641 acctcgggct atttcggcgg acgcatcgac acgtttctgc agcgcatcgt cgacatcatg
     5701 ctgtcgttcc cgatcatcat ccttgcagtg gtcgtcgtcg ccgtgctcgg caagtggccg
     5761 gtcttcgacg tcgacatgaa cctcatcatc gccatagcgc tgcccatgat cccgaagatg
     5821 gcacgcatcg cgcgctcgtc caccttgtcg atcgtgcaga tgccgttcat cgatgcggcg
     5881 agggcagcag gcttcgggca tacgcgcatc atcctgcgcc acatcatgcc gaacatcatc
     5941 gcgccctacc tcatcatggt gacggcgttc atcgcgcaag ccatcctgct cgaggcgagc
     6001 ctctcgttcc tcggcctcgg cgttaccgag ccgacgccgg cctggggcct catgctgtcg
     6061 ggaagctcag ccgacttcgc ccgcacggcg ccctggatga tcatcttccc gggcctcgcc
     6121 atcacgctcg ccgtgttcgc cttcaacctc ctcggtgaca gcctccgtga ttggctcgac
     6181 ccgaagatga agggctgaag gcagcgatcc ctgcaggcat cgcgtagctg atctgaggat
     6241 gctgcgcgca tcagtgcgcg ggcctgtgat agcgtcacgc ctcgatggct tgagcagtgt
     6301 cgcagcagga ggacgccgat ggacttcaag ggcaaggtcg ccgtcgtgac cggggcggcg
     6361 aacggcatcg gacgggcaac ctcgtttgcg ttcgccaagg ccggcgccaa ggtcgtgctc
     6421 gtcgaccgcg atgaggccgg cgccaaggct gcggccggcg ccatcgtcca gcagggcggc
     6481 acagcccacg gcgtcaaggc ggatgtcacc cgctccgccg atgtccaggc ctacgtgaag
     6541 gccgccctcg aaacgtacgg ccggatcgac gccttcttca acaacgccgg catcgaaggc
     6601 aaggtcacgc cgatcgtcga gtacgacgag gagatgttcg atcaggtgat cggcatcaat
     6661 gtgaaaggcg tgttcctcgg gctgcgctac gtgctcccgg tgatgctgaa gcagggcagc
     6721 ggcgcgatcg tcaatacggc ctcggtcgcc ggcatcgtcg gcacgccggg catcgcgccc
     6781 tatgtcgcct ccaagcacgc cgtgatcggc ctcaccaaat cggtttccgg cgaagtggcg
     6841 cgccagggcg tacgcgtcaa tgcggtctgc ccgggccccg tcgacacgcg catgatccat
     6901 tcgctcgagg agcagatcaa tccggccgat cccggcagcg tcggccagcg ctacaagggt
     6961 gcgatcccgc ttgggcgcta tgcgaccgtc gacgagatcg ccaacatggt gctgttcctt
     7021 tgctccgatc tcgcctcggc catgaccggc ggccattacg ttgtcgacgg tggacgcacc
     7081 gccacgggcg gcgctgtgac caacgtcgcc atgannnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnncaaac tccaaaaagg tcgtcgtccc gaacaccatg
     7201 tgcaaggccg gcatcgagat cctgcggtcg cggcccgagg tgacggtcgt cgagttcgac
     7261 aacttcgtct cctcggcgga cttccagaag tttcttgccg cacaaggcgg cgtgcacgcc
     7321 ggcattctcg gcgcgacgcc gtggagcaag ggtgagtgcg aagcggccaa gacactcgag
     7381 gtcgttgccc gcatcggcgt cggcttcgat gccgtcgatg taccggagct cacgcggcgg
     7441 aagatcccgc tgatggtggt cggtaccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnc gcatcggcac gcggacggcg aagcgcgcgg cggccatgga gatgaccgtc
     7681 cagatctacg acccgtacgt gaagccggag gtcatcaagg ccgccggcta cgagccggta
     7741 aaggacctcg acgcagcact gccgcgcgcc gatttcgtct ccatccactg cccgaagaat
     7801 gccgagacga agggcatgtt cgacgggaag cgcctggcgc tcctgaagcc gacggcgtac
     7861 ctcgtcaaca ccgcgcgcgg cggcctcatc gacgaaatcg cgctgcacaa ggcgctgacc
     7921 acgggcaagc tcgcaggggc gggccttgat gtgttcgaca gcgagccggt gaaacccgac
     7981 aacccgctcc tgaagctcga caatgtgatc acggcgccac acatggcggg cgttacgctc
     8041 gaatccgtcg atcgcatggg ccagcaggcg gctcgcaatg tcctttccgt gttcgaccgc
     8101 gcgatcatcc gtgagaatgt ggtcaaccag gagattctga agtaggattc ttgaacatcg
     8161 tgccacggct cgcagccctc actcgccttt ccgctacgtg tgccaaggcg gaatacgggg
     8221 aagcatgggc cggcatggtg tgcggagctg gttgcgccgg tctggtacat cgggccggcg
     8281 cgctcaagtg cacgggtccg atcgctaggg ggtgttgagc ccatgcctgc acgacagacc
     8341 attgcgatcg acgcgatggg cggagacttc ggtcccgagg tgaccatccc gggtgctgcg
     8401 ctggccgccg agcgccaccc caacgcgacc ttcgtcttct acggcaacca ggcgcggatc
     8461 gatgccgccc tggtgcaaca cggtgcgctc aagggacgct cgcgcgtggt ccacacggac
     8521 ctggtcgttg ccatggacga caagccgagt caggccatgc gccgcggcaa gggctcgagc
     8581 atgtggctcg cgatcgaggc ggtgcagaag ggcgaagcgc acgcggcggt atctgccggg
     8641 aacaccggcg cgctgatggc catggcgaag ctcatcctgc gcacgctgcc tggcatagag
     8701 cgcccggcga ttgcagcgct ctggccgacg gtcaacggcg aatgcgtcgt gctcgacgtc
     8761 ggtgcgaaca tcggcgctag tgcgcgacag cttgccgatt tcgccttaat gggcgccgcc
     8821 atggcccgtg ccatcttcca catcgaacgt ccgaccgtcg gcctcctcaa tgtcggagtc
     8881 gaggagatca agggcgccga ggaggtgaag gaggcgcatg cgctcctgaa gacgccgagc
     8941 ctcgagccgc cgttcacata caagggcttc gtcgaaggca acgagatcgg ccagggagcc
     9001 gcggacgtgg ttgtcgtcga agggtttgcc ggcaacatcg cgttgaagac cgccgagggc
     9061 acagcgcgcc aaatcgggca ttggctcaag tccgcgctca cacaatcgat catctcgaag
     9121 cttggcgcga tcatggcgca gggcgcgctg cgcgccttca aacgcaagat ggaccccggt
     9181 cgggtcaatg gcggcacgtt ccttggcctc aatggcatcg ttatcaagag ccatggcagc
     9241 gccgacgcgc cgagctttgc gggcgcagtc gatctcggct acgacatggc cgaaagcgcc
     9301 ttgctcgagc ggcttgccac cgacatcgac gcactgcacg ggaaaattga gaccgaagcg
     9361 caggtgaccg cataggctgg ccgttcacca cacggcgaaa cgcggtccgg cagcgggccg
     9421 ggctcggagt agacagcagc ggagcttgtc ggcgaggcaa cctgccttgc cggcagtgga
     9481 gcgctgcagc aggaacgagg tgagacgtgg ccgtcattcg atccgtgatc aggggcgtcg
     9541 gcgcctatct gccgaagcgc gtgatgacca acgacgacat ctcgcggatc gtcgacacgg
     9601 acaacgactg gatcgtcgag cgcacgggca tcaagtcgcg ccacattgcg gccgagggag
     9661 agttgacctc ggacctcggc atcatggcct cgaagcaggc gcttatccgg gctggcatcg
     9721 atcccgtgga tatcgacctc gtcatctgtg cgacggccac acccgaccgc acgtttccgg
     9781 cgactgccgt caagattcag tcggcgctcg gcgtgaccaa gggggctgca ttcgacatcc
     9841 aggccgtctg ctcgggcttc gtctatgccc tcgcgaccgc cgacaacttc ctgcggaccg
     9901 ggcagttcaa ccgtgctctg gtcgtcggcg ccgagacgtt ctcgcgtatc ctcgactggt
     9961 ctgaccgtgg cacctgcgtt ctgttcggtg atggcgcagg cgcggtggtg ctcgaggcgc
    10021 agccacagcg aggccagaag acggaccgcg gtatcctgca ctcgatcatc cgctccgacg
    10081 ggcgctacga ggaccttctt tacgtcgacg ggggaccggg ctcaacgaag acggtcggcc
    10141 acctgcgcat gaacggccgt gaggtattcc gccacgccgt gcagaagatc tcgggcgtga
    10201 tcgaggagac gctcgttgca accggctatt ccgccgagga gattgatctc ttcgtgccgc
    10261 atcaggcgaa caagcgcatt ctcgacggca tcgccaagcg gctcaatgtt gccccgggca
    10321 agatcgtcat gtcgctcgat cagcatggca acacctcggc cgcgtccatc ccacttgcct
    10381 tgaaccaggc cttcgaggcg cacaaagtca aggaaggcgg cctcatcatg atggaggcga
    10441 tgggcggcgg cttcacttgg ggcgccgtgc ttctccgctg gtagttgcga cgatgcacag
    10501 gcccggagag ttctgtcgta atttcagata tttagtgcgg cccgatcttg acccgccaag
    10561 tcgtggtcac tagcatccgg tgaaacaacg aggggaggcg aacgaaatgg ggaggccagt
    10621 acccatgggg catcgcacat tgacgcgggc cgatcttgcc gaggcggtgg tgcagaaggt
    10681 cggcttgccc aggaacgaga gccaggatct cgtcgagctg gtgctcggcg agatttcgac
    10741 gtcgctcgcg gaagggaagg ccgtcaagct ctcctcgttc ggatcgttcg gcatccgctc
    10801 gaagggggag cgcgtcggcc gcaatccgaa gaccggcgag gaagtgccga tcacaccgcg
    10861 gcgtgtgctt gtgttccgac cgagcaacat catgaaggac cgcatcaacg cgggcaggtt
    10921 gcggcggaag ccatagccgc attggttggt cggagcgcgt cgcagacctg ggaacagcga
    10981 tcgttgcgtc ggtcggtatg tctctgggag caaaagaagc gtcgcgaact ggggggcggc
    11041 acgtcggtaa gcggcacgtc ggtaaagagc aatcgtcgga cgcagccgaa tggtcgtgtc
    11101 gacccgcatc ggcttgcact ttggatgaca tagcgctgca ggtcgcccag acgaggagcg
    11161 atcaagacga agtgcggcca agacgaagtg cggccaagac ccagtgtccc gggctcggct
    11221 gtgtcgggag cccgcacaat ttcgcgttgc aagtcctcgc gttgcaagtc attgaacgat
    11281 ctcatgcgaa ggagcccgcc atggcccgcg ccgcagtgac tgccgacaag agcactgacg
    11341 cctttcgcac gatcagcgag gtggcagaga gcctcgacgt gccgaagcac gtcctgcgct
    11401 tctgggagct caaattcacg cacatccgcc cgatgaagcg aggcggcggc cggcgcttct
    11461 atcggcccga ggatgtcgag ctactgcgcg gcatccagta cctgctgcac ggtgagggct
    11521 acacaatccg cggcgtgcag aagatattgc gccggcacgg tatcgagctc gtcaagtcgt
    11581 gctggcgcga tggtgcggcg caactgcgcc ccgagggctt cgaacgtgag gagcccgggg
    11641 cgcggacgca gaaggccggc gcaactgccg ccaaggcggg aaagaggcga gacacggcat
    11701 tgctgagcga ggatggcggc caagcgctca tcgcctcggc gattcgcgaa ctcaccgcat
    11761 gtcgtgagct gctcacgggc ctgctccgtg ccgaaggcag cgccaaggga cggccgggct
    11821 gagacatgcc ggcgcaacgg caaggtgaag tttggcgcgt cgattgcgag gaggtcgcgc
    11881 gacacggcct cgatggcgcg cgcggcatcg atatcgttcc ggcggacgag atcgagcgca
    11941 gccggacgtt tgccgatcgt gaccggcgac gccactggct cgcgctccgg attgcactcc
    12001 gctcggctct ggtgccctgg atcggcagaa ccgatgccgc ggggccgttc cgtctgggcg
    12061 cagcggggcg gccggctctc aacggacatg ggccgagctt ttcgatctcg cataccggcc
    12121 cactcgggct cgttgcgatc gccacggaca gcatcggcgt cgacatcgag ccggagcggg
    12181 ccgctgccat tgcagcctgg cgacgctccg cgatcgaggc ggcggcgatc atgctcgccc
    12241 cgggtttggc tctgcccgat gctcccgagc cgcgcttcat ccaatgctgg gtacggctcg
    12301 aggcctacgc caaggcgagc ggctggggga tcggccgcac gctcgtcgaa cttggtgccg
    12361 cgggagctcg gcaaggggag cctgcggggg gtgaccagcc gagcagccca ggtggcccgg
    12421 ctgaggcctc gcccaattgg agcgtcgtgg acttg
//
LOCUS       DAORNH010000117        12455 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3836_length_12455_cov_2.468710, whole genome shotgun sequence.
ACCESSION   DAORNH010000117 DAORNH010000000
VERSION     DAORNH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12455
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_12455_cov_2.468710"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1753
                     /locus_tag="PK264_12850"
     CDS             113..1753
                     /locus_tag="PK264_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD76802.1"
                     /translation="MRDDHLFGIGRRQRYAEMRRDFRWSVPEHFNFATDVVDAWARER
                     DGIAVIAEDAQGEVRTLRFSDVSRASMKLADALRSRGVEKGDRVIVMLPRMPEWQIAL
                     VACLRIGAVAIPCIEMLTAKDIAYRVANSGAKAAICRADHAVKFEEVAEKVPVRIAIG
                     GAPGWIDYHAATGAAREDVAAAVVAADDPAVMYYTSGSTGHPKGVLHASRGLYTWRMS
                     AFYWLDLTPEDRIWCTADTGWSKAGTSIIWGPWSCGAAAFIYDGPFSPAERLRLLAKH
                     KITVFCAASTELFRLVDEDPKRHDLSSLRHAITAGEALNAVVARRFEEVFGVKVAEAY
                     GQTEALMMTLNFPSEPLRYGSMGLPGPGIHLDIVDDEGRRVADGVEGHVAILTPCPQM
                     MLGYWQDPARTAECFIDGPDGRWYISGDRAHRDRDGYLWYDGRSDDVINSAGYRIGPL
                     EVENVLLDHPSVQECAVVASPDVERGEVVKAFVVLRKGVSGTRELIAELQSHAKAATA
                     PYKYPRRIEFITELPKTITGKIRRRALRDLEVERGRKG"
     assembly_gap    2060..2069
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2070..2821)
                     /gene="dnaJ"
                     /locus_tag="PK264_12855"
                     /pseudo
     CDS             complement(2070..2821)
                     /gene="dnaJ"
                     /locus_tag="PK264_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933603.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
     assembly_gap    2822..2945
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2946..3112)
                     /locus_tag="PK264_12860"
     CDS             complement(<2946..3112)
                     /locus_tag="PK264_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012559503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRD76803.1"
                     /translation="MAKRDYYEILGVTKSANEQDLKSAFRKLAKELHPDRNPDDKDAE
                     RKFKEAAEAYEV"
     gene            complement(3171..>3436)
                     /locus_tag="PK264_12865"
     CDS             complement(3171..>3436)
                     /locus_tag="PK264_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76804.1"
                     /translation="AAITELREALKGENVETIKAKTQAVAQAAMKLGEAIYAPGGAGA
                     PNGDGDGSASGAAPGGPGAKKAGDNVVDADFEEVKDDPKKRSA"
     assembly_gap    3437..3487
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3488..5107)
                     /gene="dnaK"
                     /locus_tag="PK264_12870"
     CDS             complement(<3488..5107)
                     /gene="dnaK"
                     /locus_tag="PK264_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRD76805.1"
                     /translation="MSKVIGIDLGTTNSCVAIMEGGQPKVIPNAEGHNTTPSIVAFTD
                     DGERLVGIAAKRQAVTNPTHTLYAIKRLIGRRFNDGEVEKDKKLAPYKIVNSTNGDAW
                     VESRGKQYSPSQISAFILMKMKETAEAYLGQPVTQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKRESKTIAVYDLGGGTFDISILEISDGVFEVKSTN
                     GDTHLGGEDFDQRIVDYLLEEFKKANGIDLAKDKFALQRLKEAAEKAKIELSSSIQTE
                     INLPYITADQSGPKHLALKLTRAKLESLVDALIERTKEPCRKALTDAGLKAAEIDEVV
                     LVGGMTRMPKVQQIVKEVFGKEPHKGVNPDEVVAVGAAIQGGVLKGEVKDVLLLDVTP
                     LSLGIETLGGVFTRLIDRNTTIPTKKGQVFSTAEDNQQAVTIRVFQGEREMANDNKLL
                     GQFDLVGLPPAPRGVPQIEVTFDIDANGIVNVQARDKGTGKEQSIRIQANGGLSEAEI
                     KKMAEDAERFKDEDKKKREIVEARNQGEALIH"
     gene            5426..5644
                     /locus_tag="PK264_12875"
     CDS             5426..5644
                     /locus_tag="PK264_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76806.1"
                     /translation="MRHVKFIAVTVIGLALIFKALFLTGKIGLKYWYVARHDWTTLLF
                     VIGGIIVVAVLWKLFRVWWVSRNVKVVR"
     assembly_gap    5807..5816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6067..6855
                     /locus_tag="PK264_12880"
     CDS             6067..6855
                     /locus_tag="PK264_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76807.1"
                     /translation="MLQSVLEAWKLSLHHRGSMLKITMPLVGITFAYFAANAVAHHAQ
                     RRLADPVVLFWAFATLYSTITAAVSWHRLLLLGEPIKAIRKWHSADTAYLKGSLLIAL
                     IATAVFIPIVLIMALIAPAPTKTPWGAQPAAPVKTMLALGLATMLTVAIISARTMVLL
                     PGRAIGNKMRATEAFNYTRGNTFRIVLAMGAAIAPLIALTQLIQLGAAYAFLFGMIAE
                     AYQLPAVILLILVIMTIQVIYSFVALTMASVLYGKLKPVTAPVA"
     gene            7008..8132
                     /locus_tag="PK264_12885"
     CDS             7008..8132
                     /locus_tag="PK264_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRD76808.1"
                     /translation="MPLDPVRLTQELIRFDTVNPPGQERACAEHLGRLLEAAGYACAY
                     VEMGEGRASLIARIGGSNDRLPIAMTGHIDVVPLGTRPWSVDPFGGEIGQGRVYGRGA
                     SDMKAGVAAIIAAAVDRASELAKGPGAVLIITAGEETGCTGAHHIVTTARDLLGRAGA
                     LLVAEPTSNRPCVGHKGALRLKVTSRGATAHASMPEKGINAVYKAARAIAKLEAFDFN
                     VARHPVMGGPTLNVGWVHGGQNINSVPDLAEVGVDIRTIPSQSHARIEEEIRSYVGTE
                     ASVETLDEAVPVWTEPDVAWVRDVFAVTSNVTGEPTSVAAAPYFTDASALTPALGGIP
                     TVILGPGPPEMAHQTDEYAEVRRIEEATEITRQLVSSWRG"
     gene            complement(8143..11616)
                     /locus_tag="PK264_12890"
     CDS             complement(8143..11616)
                     /locus_tag="PK264_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HRD76809.1"
                     /translation="MFTKLMSARRFAPLFWCQFFSALNDNILKNALGMLILFGIGTTA
                     AQSPETASILITLSGVIMLAPFFILSALGGEIADRYDKGTVARWLKLAEIPVAVLAAI
                     GFVMHSIPVLFVALAGFGIIGALFGPLKYGLLPEKLAENELAAGNALVEGATFLAILT
                     GTILGGLAITTLKSAELVIGVVLVLAVLCWLSARWIPVTGPAAPDLVINRNIAASTFK
                     LLGELKADRRLWTGANIVSWFWLLGFVVLSLLPSMIKDIAGGNELAVTTALALFTIGI
                     ATGSALAAKASHDRPNLSLVPMGALLMAFFCGWLAWLVFAMKGDPTSVITRAKLGPMD
                     LFTTVSGAQLGIALFGLAASGGLFIVPAFAAFQSWAPADKRARVIASNNVLNAAYMVS
                     AGAVVAGLQFMGVGAAILLTLMSFGCLVSMVLVLRAWGKEGVRDLARGMFQALLKLEV
                     KGAEHLPKPGTRSIIAPNHVSLIDGPIMHAILPGHAAFAVDSGIAETWWAKPFLRLIN
                     AFTLDPTKPLAARSLVNLVREGQSLVIFPEGRITVTGGLMKVYDGTAMIADKADAVIV
                     PVRIEGPERSPLGYMKKGEIRKALFPKTTVTVLPPRKLAIGADLKGKARRQAAGAALQ
                     DIMVDTAVETARLDRSLFEALVEARDTKATKKTLVEDPLGTKLTAKKLVVGAQVLGAK
                     LAPLAAEGASVGVLLPNAAGVAVTFFALQSIGRVPAMLNFTAGAANIVSACRTARVEV
                     VLTSRAFIAKGRLEPLIAKLGEVVKIVYLEDIRPTITTGDKFRGLLAGGKPQVARKPD
                     DPAVVLFTSGSEGAPKGVVLSHRNLLANVAQCLSRISANGEDKVFNALPVFHSFGLTG
                     GLLMPILGGIPVYLYPSPLHYRIVSELIYGTNSTIVFGTDTFLSGYARVAHPYDFHNV
                     RLIMAGAEAVKDRTRQAYMERFGVRILEGYGVTETAPVLAMNTPLANKSGTVGRLSPL
                     MEARLDPVPGITEGGRLFVRGPNVMLGYYRAENPGVIEPPAGGWHDTGDIVTFDAEGY
                     IKIRGRAKRFAKIAGEMVSLSAIEALAAEVWPQAISVVVAEPDARKGERLVLMTQEAK
                     ATREAFQRHARARGATELMVPAEIMAGAAIPLLGSGKPDYVAAAEKARSRVRPAAPAE
                     AEADDTADAAA"
BASE COUNT         1997 a         4094 c         3999 g         2170 t
ORIGIN      
        1 ggcctctttc gcagccggct ctggcggtgg ctggctcttg agctgcgcag tcgtcttggc
       61 catgatgacg cctcgcggcg cgcaaggcgc cctgatgtcg cggaggcgag gcgtgaggga
      121 cgatcatctg ttcggcatcg gccggcggca acggtatgcc gagatgcgcc gtgacttccg
      181 ctggtcggtc ccggagcact tcaacttcgc gaccgacgtg gtggacgcct gggcgcggga
      241 gcgcgacggc atcgccgtaa tcgccgagga cgcccaaggc gaggtgcgca cgctgcgctt
      301 ctcggatgtc tcgcgtgcct ccatgaagct cgccgatgcg cttcgctcgc gcggcgtcga
      361 aaagggcgat cgcgtcatcg tcatgctgcc gcgcatgccg gagtggcaga tcgcgctcgt
      421 cgcctgtttg aggatcggcg cggtcgccat cccctgcatc gagatgctga cagcgaagga
      481 catcgcctac cgtgtagcga actcaggcgc caaggccgcg atctgccggg ccgaccatgc
      541 cgtcaagttc gaggaggtgg ccgagaaagt gccggtccgc atcgccatcg gtggggcacc
      601 gggctggatc gactatcatg cggcaaccgg cgccgcgcgg gaggatgtgg cagccgccgt
      661 cgttgccgcc gacgaccctg ccgtgatgta ctacacgtcc ggctccaccg ggcacccgaa
      721 gggcgtgctc cacgcctcgc ggggcctcta cacgtggcgc atgtcggcct tctactggct
      781 cgacctcacg cccgaggacc gcatctggtg caccgccgat accggctgga gcaaggccgg
      841 gacgagcatc atctgggggc cgtggagctg cggcgcagca gccttcatct acgacgggcc
      901 attctcgccg gctgagcgcc tccgccttct cgccaagcac aagatcacgg tgttctgcgc
      961 tgcaagcacg gaactcttcc gcctcgttga tgaggatccc aagcgtcatg atctctcgtc
     1021 gctccggcac gccatcaccg caggcgaagc cctgaacgcg gtcgtcgcgc gccgcttcga
     1081 ggaggtgttc ggcgtcaagg tcgcggaggc ctacgggcag accgaggcgc tgatgatgac
     1141 gctgaacttt ccctccgagc cactgcgcta cggctccatg ggccttcccg ggcccgggat
     1201 ccatctcgat atcgtcgacg atgaaggacg ccgtgtcgcc gacggcgtcg agggtcacgt
     1261 cgcgatcctc acgccatgcc cacagatgat gctcggctac tggcaggatc cggcgcgcac
     1321 cgctgagtgt ttcatcgatg ggccggatgg ccgctggtac atctcgggcg accgcgcaca
     1381 ccgtgatcgt gacggctacc tctggtacga tggccgctcc gacgacgtca tcaactcggc
     1441 cggctaccgc atcggcccac tcgaggtcga gaacgtgctg ctcgatcacc cgagcgtgca
     1501 ggagtgtgca gtggtcgcga gccccgatgt cgagcgcggc gaggtcgtca aggcgttcgt
     1561 cgtgctgcga aagggggtct cgggcacgcg cgagctcatc gccgagttgc aatcccacgc
     1621 aaaggcggca accgcgccct acaaataccc gcgccgcatc gagttcatca ccgagctgcc
     1681 gaaaaccatc accggtaaga tccgccgccg tgcgctgcgc gatctcgagg tcgagcgggg
     1741 acggaagggg tgagaggcgt gcgccagtga agcccctcca ctgtcacccc ggacgccgaa
     1801 gcggcccttc cgccgcgcag gcgatccggg acccagcgcg aggagcagcg aagctgccgt
     1861 aacgctccca cccgcaatat ctcgcgctgc gcgcgcttct tgtgctgtgt cccggctcgg
     1921 cgcgttcgcg ggcgctcacg ctaggccggg atgacagcac cgccgtctta cgcccccttg
     1981 ccgtcgaaga actcgcggac gcgggcgaag aagccggtgg tcgtcgggct gttctcggcc
     2041 ttgctctcct tgtcgaactn nnnnnnnnng ctggcggcgc gtcaggttct tcggcgtctc
     2101 gacctcgacc tgaatataca tgtcgccgtg gttcgaggtg cgcagaacgg gcatgccctt
     2161 gcccttgagg cggaactgct tccccgtctc cgagccctcg ggcaccttca cgcgcgtgcg
     2221 accgccatcg atggtcggca cctcgatctg gccgccgagg gccgcggtca ccatggagat
     2281 cggtacgcgg cagaagatat cggcgccgtc gcgctggaag aactcgtgcg gcttgatcgc
     2341 gaggaagatg tagagatcgc cggccgggcc gccgcgcagg ccggcctcac cctcgccggc
     2401 aaggcggatg cgcgtgccgt cctcgacacc cgccgggatc ggcaccgaga gcgtgcgctc
     2461 cttggtgacc cggccgatgc cgttgcacga ggcacacgga tcgtcgatca cctcgccgcg
     2521 gccctggcag gaggggcatg tccgctcgat cgtgaagaaa ccctggctcg cgcgcacctt
     2581 gcccatgccg gcgcaggtgc cgcaggtctt gggcttggtg ccggccttgg cgccagtgcc
     2641 tgtgcagggc tcgcaagtga cgctcgtcgg cacgcgaacc tgtgccgtgc ggccggtgaa
     2701 ggcctcggtc agcgtgatgt ccatatggta gcggatgtcg gcgccgcgct cgcggcccga
     2761 gcgctggcga ccgcggcggc ctcccatgaa ctcaccgaac agatcgtcga agatgtccga
     2821 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnacctc gtaggcctcc gccgcctcct tgaacttgcg ctcggcgtcc ttgtcgtccg
     3001 gattgcgatc cgggtgcagc tccttggcga gcttgcggaa tgccgacttc aggtcctgct
     3061 cgttcgcgga cttggtgacg ccgaggatct cgtagtagtc gcgcttggcc atgcgatggg
     3121 tctttcaatc gctggagtgg acgcgccccg gaggaccggg gcgcaccgca tcaggccgaa
     3181 cgctttttcg ggtcgtcctt tacttcctcg aagtcggcgt cgacgacgtt gtcaccggcc
     3241 ttcttggcgc cggggccgcc cggagccgct cccgaagcag acccgtcacc gtcaccattt
     3301 ggggccccgg ctccgcccgg cgcgtagatc gcctcgccga gcttcatcgc cgcctgcgcg
     3361 acagcctgcg tcttcgcctt gatcgtctcg acgttctcgc ccttcagcgc ctcgcgcagc
     3421 tccgtgatcg ccgcctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnngtg gatgagcgcc tcaccctggt tgcgcgcctc gacgatctcg cgcttcttct
     3541 tgtcctcgtc cttgaagcgc tccgcgtcct cggccatctt cttgatctcg gcctcgctga
     3601 gaccgccatt ggcctggatg cggatcgact gctccttgcc cgtgcccttg tcgcgcgcct
     3661 gcacgttgac gatgccgttc gcgtcgatgt cgaacgtcac ctcgatctgc ggcacgccac
     3721 gcggcgccgg cggcagaccg acgagatcga actggccgag cagcttgttg tcgttggcca
     3781 tctcgcgctc gccctggaag acgcggatcg tcaccgcctg ctgattgtct tcagcggtgg
     3841 agaagacctg tcccttcttg gtcgggatcg tggtgttgcg gtcgatgagg cgcgtgaaca
     3901 cgccgccgag cgtctcgatg ccgagcgaca gcggcgtcac gtcgaggagc aggacgtcct
     3961 tcacctcgcc cttgagaacg ccgccctgga tggcggcgcc gacagcgacg acctcgtcag
     4021 ggttgacacc cttgtgcggc tccttgccga acacttcctt gacgatctgc tgcaccttcg
     4081 gcatgcgcgt catgccgccg acgagcacga cctcgtcgat ctcggcggcc ttgaggccgg
     4141 cgtccgtgag cgccttgcgg cagggctcct tggtgcgctc gatgagtgcg tcgacgaggc
     4201 tctcgagctt tgcccgcgtg agcttcagcg cgaggtgctt cggacccgac tggtcggccg
     4261 tgatgtaggg caggttgatc tcggtctgga tcgaggagga gagctcgatc ttcgccttct
     4321 cggccgcttc cttcaggcgc tggagcgcga acttgtcttt ggcaaggtcg atgccgttcg
     4381 ccttcttgaa ctcctcgagg aggtagtcga cgatgcgctg gtcgaagtcc tcgccgccga
     4441 ggtgcgtgtc gccgttggtc gacttcacct cgaacacgcc gtcgctgatc tcaaggatcg
     4501 agatgtcgaa cgtgccgccg ccgagatcgt agaccgcgat ggtcttggat tcccgtttct
     4561 tgtcgaggcc gtaggcgagc gctgccgccg tcggctcgtt gatgatgcgc agaacttcga
     4621 ggccggcgat cttgccggcg tccttggtcg cctgacgctg cgcgtcattg aaataggcgg
     4681 gcaccgtgat cacggcctga gtgacgggct gtccgagata ggcctccgcc gtttccttca
     4741 tcttcatcag gatgaaggcc gagatctgcg agggcgaata ctgcttgccg cgcgattcca
     4801 cccacgcgtc accgttcgtc gagttgacga tcttgtaggg cgcgagcttc ttgtccttct
     4861 cgacctcgcc atcgttgaag cggcggccga tcaggcgctt tatggcgtag agcgtgtggg
     4921 tcgggttggt taccgcctgg cgcttggccg cgatgccgac gagccgctcc ccgtcgtccg
     4981 tgaacgcgac gatcgacggc gtcgtgttgt gaccctcggc gttcgggatc accttcggct
     5041 gaccaccctc catgatcgcg acgcacgagt tggtcgtgcc gagatcgatg ccgatcacct
     5101 tggacatatg cttgacctct tgagttgcgg cggaccaacc aggccctggc gctcaggacg
     5161 cttacggcac ccgctatgac ggcagccctc ccgggctccg tgccggtccc cttcagtgag
     5221 ttggccgtga tataggcttg ggccatcacc gctgcaacgg atgaggaagc cgcaacggtg
     5281 agtttttgtg acccgattgc agaggctgtg gggcaggcgg gggacgagct gcggcatcac
     5341 gggccggctc gatcgcgcag cgcgcagcga attcgatttc tggtcggccc aaggcatgat
     5401 cagatttggg gcggaggagc cggccgtgcg ccatgtcaag ttcatcgccg tcacagtgat
     5461 cggtttagcc ctaattttca aggcattgtt cctgaccggg aagatcggcc tcaagtactg
     5521 gtacgtggcg cgccatgact ggacgacgct gctcttcgtc atcggcggca tcatcgtcgt
     5581 cgccgttctc tggaaactgt ttcgcgtctg gtgggtgagc cgcaacgtca aggtcgtccg
     5641 gtaacggacg agcatcgccg ctccttcgag cctgcggcca aattgtcaca gcatgacgca
     5701 ccgaaacggt ggcacccgga tgggcgccgc cgcgaatcgt ttgtcgcgcg cttcgcgacg
     5761 cccgctcgag cctgcagatg gcgcggggcc cgcctgtgcc gggattnnnn nnnnnnagac
     5821 gggccccggg tgggccggcc attgctggcc gggtgatcca caccgttccc ttgcgcccgc
     5881 cagaaactaa ttgatcaaag ttcacagatt ctcggcagcg cacgctcgtc gagcatctcg
     5941 ccaaggttga ttcgcgtgcc ggcatcgtga tcattgttca aaattggctc tggtctgatc
     6001 attcatctct cgttaaccca gtcgaggaga gacgacgatg acaaccaacg cccccagaag
     6061 cttcccgtgc tgcagagcgt gctcgaggcg tggaagctca gcctccacca tcgcggcagc
     6121 atgctgaaga tcacgatgcc acttgttgga ataacattcg cctacttcgc tgcaaacgcg
     6181 gtcgcccacc atgctcaacg tcgcctcgca gatccggttg tgctgttctg ggccttcgca
     6241 accctctact ctacgatcac agcagcggtt agctggcacc ggctcctgct cctcggcgag
     6301 ccgatcaagg ccatcaggaa gtggcatagc gccgacacgg cctacttgaa gggatcgctg
     6361 ctgatcgccc tgatcgcgac ggctgtgttc atcccgatcg tactgatcat ggcgctgatt
     6421 gctcctgccc cgacgaagac cccttggggc gcccagccgg cagcaccggt gaaaacaatg
     6481 ctcgcattgg gcctcgccac aatgttgacc gtcgcaatca tttctgcgcg caccatggtc
     6541 ctcctgcctg gccgcgccat cggcaacaag atgcgcgcca ccgaggcctt caactacacc
     6601 agagggaata cattccgcat tgtccttgcg atgggcgccg ctattgcacc cctcatcgct
     6661 ctcacccaac tgatccagct tggcgctgcc tacgcattcc tgtttggaat gattgccgaa
     6721 gcataccaac tccccgccgt cattctgctg atactcgtga tcatgaccat ccaggtgatc
     6781 tactcgttcg tcgctctcac gatggcctcg gtgctctacg gcaagctgaa accggtcacc
     6841 gcgccagtcg cctgaagtgc acgacctgga catcgcactg acaagaacca tctcgcccgg
     6901 atcacatggt ccgggcggta tcgtttcagg ccaaggacga cggcaccgct tgtcagcctc
     6961 gccgcgccgc gccacggttg gcggacccgc gatccccgag gaccaccatg ccgctcgacc
     7021 ccgtccgcct cacgcaagag ctgatccgct tcgacaccgt gaacccgccc ggtcaggagc
     7081 gcgcctgcgc cgagcatctg gggcgcctcc tcgaggccgc gggctatgct tgcgcctacg
     7141 tcgagatggg tgagggtcgc gccagcctga ttgcccgcat cggcgggtcg aacgaccgcc
     7201 tccccatcgc catgaccggc cacatcgacg tcgtgcccct cggcacacgg ccctggagcg
     7261 tcgacccgtt cggcggcgag atcgggcaag gccgggtcta tggccggggc gcctccgaca
     7321 tgaaggctgg cgtcgctgcg atcatcgctg cagccgtcga tagggcgtca gagctcgcca
     7381 aggggcccgg cgccgtcctc atcatcacgg ccggcgagga gaccggctgt acgggcgcgc
     7441 atcatatcgt caccacggcc cgcgatcttc tcggccgtgc cggcgcgctg ctcgtcgccg
     7501 agccgacctc gaaccggccc tgcgtcggcc acaagggcgc gctgcggctc aaagtgacgt
     7561 cacgcggcgc gacggcgcac gcctccatgc cggagaaggg catcaacgcc gtctacaagg
     7621 ctgcgcgcgc catcgccaag ctcgaggcct tcgacttcaa cgtggcccgc caccccgtca
     7681 tgggcggccc gacgctgaat gtcggctggg tgcatggcgg gcagaacatc aactcggttc
     7741 ccgatctcgc cgaggtcggc gtcgacatcc gcacgattcc ctcccagtcg cacgcccgca
     7801 tcgaggagga aatccgcagc tacgtcggca ccgaggcgag cgtcgagacg ctcgacgagg
     7861 cggtgccggt gtggacggag cccgatgttg cctgggtgcg cgatgtgttc gccgtgacga
     7921 gcaacgtcac cggcgagccg acatcggttg ccgccgcacc ctacttcacg gacgcctccg
     7981 cgctgacacc cgcgctcggc ggcatcccga ccgtgatcct cggccccggc ccgcccgaaa
     8041 tggcgcacca gacggacgag tacgcggagg tgcgccgcat cgaggaagcc accgagatca
     8101 cccgccagct cgtctcgagc tggcgggggt aggccacacc ctttaggcgg cggcgtcggc
     8161 ggtgtcgtcg gcctcggctt cggcaggggc tgcgggccgc acgcgcgagc gcgccttctc
     8221 tgccgcggcg acatagtcgg gcttgcccga tccgagcagc gggattgcgg cacctgccat
     8281 gatctcggcc ggcaccatga gctcggtcgc accgcgggcg cgggcgtggc gctggaatgc
     8341 ctcgcgcgtc gccttggcct cctgcgtcat aaggacgagc cgctcgccct tcctcgcatc
     8401 cggctcggcg acgacgaccg agatcgcctg cggccagacc tcggctgcga gcgcctcgat
     8461 cgccgacagc gacaccatct cgcccgcgat cttggcgaag cgcttcgctc gcccgcggat
     8521 tttgatgtag ccctcggcat cgaaggtgac gatatcgccg gtatcgtgcc agccgccggc
     8581 cggaggctcg attacgcccg gattctcggc gcggtaatag ccgagcatca cattggggcc
     8641 gcgtacgaac aggcgcccgc cctccgtgat gccgggtacg ggatcgagcc ttgcctccat
     8701 cagcggcgag agccggccga cggttcccga cttgttggcg agcggcgtgt tcatggcgag
     8761 gacgggcgca gtctcggtga cgccgtagcc ttccagaatg cgcacgccga agcgctccat
     8821 gtaggcctgc cgcgtacggt ccttcacggc ttccgcaccc gccatgatga ggcggacgtt
     8881 gtggaagtcg tatggatggg cgacgcgcgc gtagccgctg aggaacgtgt ccgtgccgaa
     8941 cacgatcgtc gaattcgtgc cgtagatcag ctccgagacg atgcggtagt ggagcggcga
     9001 gggatagaga tagacgggga tgccgccgag gatcggcatc aacaggccac ccgtcagccc
     9061 gaacgagtgg aacacgggga gtgcgttgaa caccttgtcc tcgccgttcg ccgagatgcg
     9121 cgagaggcac tgcgcgacgt tggcgagcag attgcggtgc gagagcacga cgcccttcgg
     9181 cgctccctcc gagcccgacg tgaacagcac gaccgcggga tcatccggct tccgcgcgac
     9241 ctgcggcttg ccgccggcga ggagcccgcg gaacttgtcg ccggtcgtga tcgtcgggcg
     9301 gatgtcctca aggtagacga tcttgaccac ctcgccgagc ttggcgatga gcggctcgag
     9361 gcgtcctttg gcgatgaagg cgcgtgacgt cagcacgacc tcgaccctgg ccgtccggca
     9421 tgccgagacg atgttggcgg caccggccgt gaagttcagc atcgccggca cgcggccgat
     9481 cgactgcagc gcaaagaacg tgacggcaac gcccgccgca ttcggcagca gcacgccgac
     9541 gcttgcccct tcggccgcga gtggggcgag cttggcgccg agcacctgtg cgccgacgac
     9601 gagcttcttc gcggtgagct ttgtgccgag cgggtcctcg accagcgtct tcttcgtcgc
     9661 tttcgtgtcg cgcgcctcga ccagcgcctc gaacagcgag cggtcgagcc gcgccgtctc
     9721 gacggcggta tcgaccatga tgtcctggag tgcggcgccc gccgcctgcc ggcgcgcctt
     9781 gcctttgaga tcggccccaa tcgccagttt gcgcggcggc agcacggtga ccgtcgtttt
     9841 cgggaagagc gccttccgga tctcgccctt cttcatgtag ccgagcggcg agcgctccgg
     9901 accctcgatg cgcaccggca cgatcaccgc gtccgccttg tcggcgatca tggccgtgcc
     9961 atcgtagacc ttcatgaggc cgccggtcac cgtgatgcgc ccttcaggga agatgacgag
    10021 cgattggccc tccctcacga gattgacgag agagcgcgcg gcaagcggct tcgtcggatc
    10081 gagcgtgaac gcgttgatca ggcggaggaa gggcttcgcc caccacgtct ccgcaatccc
    10141 cgaatcgacg gcgaaggctg cgtgacccgg caggatcgcg tgcatgatcg gcccgtcgat
    10201 gaggctcacg tgattgggcg cgatgatcga acgcgtgccg ggcttcggca ggtgctcggc
    10261 gcccttcacc tcgagtttca ggagcgcttg aaacatcccg cgcgcgaggt ctcgcacgcc
    10321 ctccttcccc caggcgcgaa ggacgagcac catggaaacg agacagccga aggacatgag
    10381 cgtgagcagt atcgcggcac cgacgcccat gaactggaga ccggcgacga ccgcgccggc
    10441 gctcaccatg taggcggcat tgagcacgtt gttggaggcg atcacgcggg cgcgcttgtc
    10501 ggccggcgcc caggattgga acgccgcgaa ggccggcacg atgaagaggc cgcccgaggc
    10561 cgccaacccg aaaagtgcga tgccgagctg agcacccgac accgtggtga aaagatccat
    10621 cgggccaagc ttggcgcgcg tgatcacgct cgtcggatca cctttcatcg caaacaccag
    10681 ccaggcaagc cagccgcaga agaacgccat cagcagtgcg cccatgggca ccagcgatag
    10741 attcggccgg tcgtgactgg ccttggcggc gagcgccgag ccggtggcga tgccgattgt
    10801 gaacagcgcg agcgccgtgg ttacggcaag ctcgttgccg cccgcgatat ccttgatcat
    10861 ggacggcagc agggacaaga ccacgaagcc gagcagccag aaccacgaga cgatgttggc
    10921 gccggtccac agccgccggt cggccttgag ctcgccgagc agcttgaacg tcgaggccgc
    10981 aatgttgcgg ttgatgacca ggtcgggcgc tgccgggccg gtgaccggga tccagcgcgc
    11041 cgagagccag cagagcacgg cgagcacgag gacgacgccg atgacgagct cggcgctctt
    11101 cagcgtggtg atggcgagcc cgccgaggat ggtgccggtc aggattgcga ggaacgtcgc
    11161 gccctcgacg agtgcattgc cggcggcgag ttcgttttca gcgagttttt cggggaggag
    11221 gccatacttc aacggcccga acagcgcccc gatgatgccg aagcccgcga gcgccacgaa
    11281 caggaccgga atcgagtgca tcacgaagcc gatcgcggcc agcacggcga ccgggatttc
    11341 ggcgagcttc agccagcgcg cgacggtgcc cttgtcgtag cggtcggcga tctcgccgcc
    11401 gagggccgag aggatgaaga agggggcgag catgatgacg cccgacaacg tgatcaggat
    11461 cgacgcggtt tcgggtgatt gggctgccgt cgtgccgatc ccgaacagga tcagcatacc
    11521 gagtgcgttc ttcaggatgt tgtcgttgag cgccgagaag aactggcacc agaacagcgg
    11581 cgcgaatcgc cgggcggaca tcaacttcgt gaacatgagg acccggggct ccttgctcga
    11641 ccgcatcgac cactcagccg gcggcgctaa cattttgaaa ctacgaacta cttctcgcgc
    11701 gtctccctta acgaagcgta cccgggcgtc ccgggagcgg acgcccgtcg gttggcgccg
    11761 ccatggaccg aagtacgact gtatcagcaa cgagccgtgg tggcagcgcg agcaacgcct
    11821 cctcgtcggc gtcgatttcg cacgcgcgga ccgtcgggcc gatcagccac cgggaaagcc
    11881 ggtcacgcag ctggacccgg ggaaccttgg agagagttcg aggcatgtgg tgtctccagt
    11941 tatgaccatc gttcacgata taatgttgaa ttgaactaag ttcaagggct cgcgatcgaa
    12001 agtgatttgc cggtggcacg cggaggtgat tttcggcgcg gagcgagacg ccattccgca
    12061 tcgcatcggg cgcggcagac aatcccgact ggtgggctcg gccgaggcac agtccgcttg
    12121 cccgagcgct ctaccgcctt ccctggccgt gcgcgctgat gcagcctgcg gcccgcgata
    12181 gacacacttt ggcaatactt tccgcccaat tctctgagca aggtctcatt taccgtcgcg
    12241 cgcgaaagct ctgtctcgcc acctcgacga gcgacgttca tcaggcacgg atcgatcaat
    12301 ggccatctcg atgtcgaaac tttccgcatg gctcgtgcgc cccacgtcag gccggacggg
    12361 ccgcggaatt gcagcgctcg gactgctcgc gatcgcggcg ccgatcctcc tctcggtcgg
    12421 cagcgtctcg ggccaggagc gactcccttg gaacc
//
LOCUS       DAORNH010000118        12453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3837_length_12453_cov_2.596951, whole genome shotgun sequence.
ACCESSION   DAORNH010000118 DAORNH010000000
VERSION     DAORNH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3837_length_12453_cov_2.596951"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PK264_12895"
     CDS             complement(<1..140)
                     /locus_tag="PK264_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HRD76810.1"
                     /translation="MRLLFVGDVVGRAGRRVVLDTLPGLRTRLALDFVVLTHAHIDHS
                     GLL"
     gene            complement(147..749)
                     /locus_tag="PK264_12900"
     CDS             complement(147..749)
                     /locus_tag="PK264_12900"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRD76811.1"
                     /translation="MSDDEILAAKKALRVKAEEARRAVLAREGTEIARRIAAHGLDCL
                     GSDARPGPETIVSGFMSMPGEIDTAPLMRWLRAEGYRLAMPAMVGKGAPLTFRSWSPD
                     DVMGKGRWGIAEPLPDKPVVFPAILIVPLLAFDRRGGRLGYGGGYYDRTLASLRRRGR
                     ITAIGLGLDELEVDAVPRLDYDEPIDWVLTESGPRRLDQG"
     gene            complement(1056..1433)
                     /locus_tag="PK264_12905"
     CDS             complement(1056..1433)
                     /locus_tag="PK264_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HRD76812.1"
                     /translation="MDASNARTAIEKGGKALGEALTARNAKAAAALYTEDAMLLPPDS
                     PIVKGRAAIEQFWGAAVAALDLKSASLSTLDVTSVGDSAIEVGTATLLLPSGPANVKF
                     VVAWKRGADGAWRLHRDIWNNGT"
     gene            complement(1658..3460)
                     /locus_tag="PK264_12910"
     CDS             complement(1658..3460)
                     /locus_tag="PK264_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD76813.1"
                     /translation="MAGKGSDKGRSRSGSGAKGGAEEKRARVKPRAERGFLRGLQNYG
                     DPDFSIYLRRAFAKSMGYSHDELKRPIIGIADTYSRFNACHRHFPELVEAVKRGITAA
                     GGLPFEFPTISVHESTLAPNSMKFRNLMAMDTEEMIRAQPMDAVVMLGACDKTVPAQL
                     MGAASAGKPAIQLIGGPQMTGRYREHRVGACTDCRKFWVEYRAGRISQAEIEAVEGRL
                     VTTAGSCAVMGTASTMAAIAETLGMTLPGTAAIPAVHADRLRAAEASGAAAVRMIAGN
                     LTPDRIITAKSVENATRVLLALGGSTNGVIHLAAIAGRLGIKLDLRRFNELSDTTPVL
                     AAVKPVGSEFYMEDFYAAGGIGAVLRELKPLLHLDCMTVTGETLGERLAAEEGAYVDR
                     RVIKPASSPVEREGGLVALFGSLAPDGCIIKRSAATPALFERTGRAVVFSSIADLSAR
                     IDSPDLDVTADDFLILQNAGPASSASMPEAGFLPIPKKLLAAGVKDMVRISDCRMSGT
                     AYGTIVLHVAPEAAIGGPLGLVRTGDRISLSVKERRIDLMVDAAELEKRRAAQPRGTD
                     DAALRGYDKLFRKSVLQAPDGCDFDFLRPASSAR"
     gene            complement(3589..>3796)
                     /locus_tag="PK264_12915"
     CDS             complement(3589..>3796)
                     /locus_tag="PK264_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76814.1"
                     /translation="ALILAALFAGHELISGELSLATSYQRSVMMLILATFALIVTFNF
                     ALWRTLRRAQASPRRRSERGRITR"
     assembly_gap    3797..3809
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4466..5260
                     /locus_tag="PK264_12920"
     CDS             4466..5260
                     /locus_tag="PK264_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76815.1"
                     /translation="MQPLTPLGIARGLLAPPILRLTGLPLRQPAPVLALIALSTAAQL
                     LSMIAEPGEPAPALDEIERHLARLEAAHMTSDATLRAAAFDLKTWVLAWRGLADGDVV
                     RLEDARTRLVTMQARACDTWSGCNRSGMLALVSLALGGEVGGAERLAEAELAASEALS
                     LAIELSEPAIIATCCALAGRTSLALGRARTDVRLLESARVRFDEAQRLSAGAWSDGAC
                     GLGDIAAEIGDVEAAIQELDAPLKRTAAPLTRDPPTLSLVRLTSRS"
     gene            complement(5267..5467)
                     /locus_tag="PK264_12925"
     CDS             complement(5267..5467)
                     /locus_tag="PK264_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76816.1"
                     /translation="MAEPTKAQQIENRAAVDAAITSEALRRRSAAAGPMKAKSATSTD
                     WIVIGVIMVVALASAFLLGRLA"
     gene            complement(5647..6102)
                     /locus_tag="PK264_12930"
     CDS             complement(5647..6102)
                     /locus_tag="PK264_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76817.1"
                     /translation="MTLPKWLAFVIAFVIATAILTVVVWAAVWAWTKISDPRKVSDRQ
                     KHGPISRITAIAMLLMIVAGLAHVGGFTTVSGVAGLSSAGLLAVSTTGLVGLVPRLKQ
                     GMMVNAHAPPRPILRWVLVPTGLLFAALSWGPGSIATIGSGLRRLCWLC"
     gene            complement(6099..6503)
                     /locus_tag="PK264_12935"
     CDS             complement(6099..6503)
                     /locus_tag="PK264_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76818.1"
                     /translation="MAYAASLFGLGVIWLAKLAADPGSDSGVGALAALVSADVFVVLG
                     AINPSCFDLGLIHITFGVDHRTVAFVLATATIFSAVVPLTCQREAKLAVGRGDVMTYI
                     RFTAFSVVATSTLSWLHLVWITRQINKWELFQ"
     gene            6769..7455
                     /locus_tag="PK264_12940"
     CDS             6769..7455
                     /locus_tag="PK264_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76819.1"
                     /translation="MAKISVTGPLAEISASCEGFGGVVTKQLADRLIDLVSAELLSFS
                     KLAVEIDALNQAFLAELNATKFYAITPAMAKLVERPQERFSDDVLRVFPEAASDIDEA
                     GRCYAFGRHTACVFHLMRVLEVPLQHLAKTLLPNDRKPNWAPIIEKIDTELKLPYKQR
                     SIIGPESFWADVSARMHAVKIAWRNRVMHVDSIVTAERASSIFDATCGLMNFLSDHAE
                     HFLHVDQPPS"
     gene            complement(7536..8486)
                     /locus_tag="PK264_12945"
     CDS             complement(7536..8486)
                     /locus_tag="PK264_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041357515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HRD76820.1"
                     /translation="MSLLSQKHLQDEEAAYQWVEAHIWPNGPVCAHCGETKRVSKMQG
                     KATRIGLYKCYACRKQFNVKIGTIFGSSHVPMHLWLQAFYLLAGSKKGISSNQLHRTL
                     GVTLKTAWFMSHRIREAMRTGTLAAPMGGNGSSGVVEADETFIGRKKGQPKRRAYHHK
                     HAVLSLVERGGEVRSFHVEKADAETIAPIVNENIAKEARLMTDEAKYYRRVGREFAGH
                     ETVNHGAGEYVRGDAHTNTLENYYSIFKRGMKGVYQHCGEQHLHRYVSEFDFRYNNRV
                     AHGVDDSERAARAVKGVVGKRLTYRPTRGEVTDAQEEIPF"
     gene            complement(8661..8876)
                     /locus_tag="PK264_12950"
     CDS             complement(8661..8876)
                     /locus_tag="PK264_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76821.1"
                     /translation="MLTPKILGALVVFVPAALFALVALRRYRPVFYFAMILIGVATGY
                     LWATGATERIWTETARYLPASVTAAVK"
     gene            complement(8933..9796)
                     /gene="fabI"
                     /locus_tag="PK264_12955"
     CDS             complement(8933..9796)
                     /gene="fabI"
                     /locus_tag="PK264_12955"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HRD76822.1"
                     /translation="MLTPGPLLAGKRGLIMGVANDRSIAWGIARAAAAHGAKLAFTYQ
                     GDAFGRRTIPLAESVGSEIIQSCDVTDLASVDAVCAEIGRKWGSLDFVVHALAFSDRR
                     ELHGRYSDTTRDNFTNTMVISCFSFTEIARRTAPLMKNGGSMLTLTYGGATRVVPSYN
                     VMGVAKAALEASVRYLAADFGQQGIRVNALSAGPMRTLAGAGISDARIIFNFQGDNAP
                     LKRTPHLDEIGGAALYLLSGLSGAVTGEVHYVDCGYNVVSMPTLQVLKEQEARLEAAS
                     QALAGKAKAAE"
     assembly_gap    9801..9810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9963..11195)
                     /gene="fabB"
                     /locus_tag="PK264_12960"
     CDS             complement(9963..11195)
                     /gene="fabB"
                     /locus_tag="PK264_12960"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HRD76823.1"
                     /translation="MRRVVVTGMGIVSSIGNSTQEVMASLREGRSGIARAEKYAELGF
                     KCQVHGEPKLDWSAAVPRKPKRFMESGLAWNYIAMDQAIRDAGLGATEVSNERTGIIM
                     GSGGPSTYAIVSAADTVRQSGGPKKMGPFEVPKAMCSGPSAVLATAFQVKGTNYSISS
                     ACATSAHCIGNAAELIQWGKQDVMFAGGCEELEWTLSSLFDAMGAMSSRFNETPTKAS
                     RAYDKNRDGFVIAGGAGVVVLEEYQRAKARGAKIYGELCGYGATSDGFDMVAPSGEGA
                     VRCMQLALKGVDGKGTPKIDYINPHGTGTPIGDQREIEAIRAVFKGEIPTISATKSLT
                     GHSLGAIGVQEAIFSLLMLNNGFVCESANIEELDPAFADVPIARKRIDGAAINCVLSN
                     SFGFGGTNATLVFKRHDG"
     gene            complement(11258..11797)
                     /gene="fabA"
                     /locus_tag="PK264_12965"
     CDS             complement(11258..11797)
                     /gene="fabA"
                     /locus_tag="PK264_12965"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HRD76824.1"
                     /translation="MAERRSSFDYQDLLACGRGELFGAGNAQLPLPPMLMFDRISQVN
                     EAGGAHGKGQIVAELKVAGNPALDWFFACHFKGDPVMPGCLGLDALWQLTGFYLGWLG
                     APGRGRALGVGEVKFTGMVLPSVGRLEYVVDLKRVILRRLKLGIGDGVMKADGKVIYE
                     ATDLKVGLFSQGEDTGVGA"
     assembly_gap    12104..12132
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12133..12358)
                     /locus_tag="PK264_12970"
     CDS             complement(<12133..12358)
                     /locus_tag="PK264_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76825.1"
                     /translation="MAPSTLSDQAARMARGAPATHVAAPPPPPPTYNLKGPLPAAPAA
                     KRSTAGGMAIQIGAYASEAEAQRQLQLAVSK"
BASE COUNT         2215 a         4142 c         3890 g         2154 t
ORIGIN      
        1 agcagcccgg agtggtcgat gtgcgcgtgg gtgaggacga cgaagtcgag cgcgagcctt
       61 gtccgcagtc ccggcagcgt gtcgagcacc acccggcgcc cggcccgccc aaccacatcg
      121 ccaacgaaca gaagccgcat ccgcacctat ccctgatcga gccgccgtgg cccgctctca
      181 gtgagcaccc agtcgatcgg ctcgtcatag tcgagacgcg gcacggcgtc gacctcgagc
      241 tcgtcgaggc cgagaccgat cgccgtgatg cggccgcgcc gccggaggct cgcaagcgtg
      301 cgatcgtagt agccaccgcc gtagccgagg cgtccgccgc gccggtcaaa ggcgagcaac
      361 ggcacgatca ggatcgccgg gaagacgacg ggcttgtcgg ggagcggctc ggcgatgccc
      421 cagcgcccct tccccatgac atcgtcgggc gaccagctcc ggaacgtgag cggcgcccct
      481 ttgccgacca tcgccggcat cgccagccgg tagccctcgg ctcgcagcca gcgcatcagc
      541 ggcgcagtgt cgatctctcc cggcatcgac atgaagccgg aaacgatcgt ctccggtccc
      601 ggtcgcgcgt ccgagccgag gcagtcgagg ccgtgcgccg cgatgcgccg ggcgatctcg
      661 gtgccctcgc gcgcaagcac agcccgccgg gcctcctccg ccttcacgcg gagcgctttc
      721 ttggccgcga ggatctcgtc atcgctcatg ccgaagtttg agcagagccc gattggcggc
      781 gcaagtgtgc gttgaccacc tgtgcgcaca gcgtgtcggt ttgagccgtc gtaggtcggc
      841 gccgatcgca gcgaagccca acggtccctt gcatcgccgt agctttgagg cgcgcgagca
      901 gttcgcttcg cgagcgaggg cccgccctcg cgctcccgcc ggcggggacg ccccgccgga
      961 ctaccccgaa tttagacgct gctcgggtct gacggacgga aactggccgg gtccgggcgg
     1021 cagcccagtt gcgcagcaat cagcgaacgc agtcgtcagg tcccgttgtt ccagatgtcg
     1081 cgatgcagcc gccaggcgcc atcggcgccg cgcttccagg caacgacgaa ctttacgttt
     1141 gcagggcccg acggcagcaa cagcgtcgcc gtgccgactt cgatagcgct atcgcccacg
     1201 gatgtcacat cgagcgtcga gagcgacgcg gacttaagat cgagggccgc gacggcagca
     1261 ccccagaact gctcgattgc cgcgcggccc ttcacgatcg ggctgtccgg cggaagcagc
     1321 atcgcatcct cagtatagag cgcggcggcc gcctttgcat tgcgggccgt cagcgcctcg
     1381 ccgagcgcct tgccgccctt ctcgatggcc gttcgggcgt tactggcatc catggcaacc
     1441 tccaaggttg gatggcaaca atcgttgagt agcacataag gatatacgcg ggatgccgat
     1501 ggccagatgg cgccggcttg gcgccaaccc actgccaacg ccaaaggagc gcgggtccgg
     1561 ggcgcgcagc cccgggcggc ctgggcctgc ggcacagctt cgacgcaagc agcacgcgtc
     1621 caactcatgc tcaattcgtc cgattgtcgc cttccaatca gcgcgccgac gacgccggcc
     1681 tcaggaaatc gaagtcgcaa ccatcgggcg cctgcagcac gctcttgcgg aagagcttgt
     1741 cgtagcccct gagcgccgcg tcgtccgtcc cgcgcggctg tgctgcccga cgcttctcga
     1801 gctccgccgc atcgaccatg agatcaatcc gccgctcctt caccgagagg ctgatccggt
     1861 cgcccgtgcg cacgaggccg agcggcccgc cgatcgcggc ctccggcgcg acgtgtagca
     1921 cgatcgttcc gtaagccgtg cccgacatgc ggcaatcgga gatgcgcacc atgtccttga
     1981 cgccggcggc gagcagcttc ttcgggatcg gcaggaagcc cgcctcgggc atcgacgcgg
     2041 acgatgccgg ccccgcgttc tggaggatca ggaaatcgtc cgccgtcacg tcgagatcgg
     2101 ggctgtcgat gcgcgccgag agatcggcga tcgaggagaa gaccaccgcc cgccccgtcc
     2161 gctcgaagag cgcgggcgtc gccgccgagc gcttgatgat gcagccgtcc ggcgcgagcg
     2221 agccgaagag ggcgacgagg ccgccttccc gctccacagg cgacgaggcc ggcttgatca
     2281 cgcggcgatc gacataggca ccctcttctg ccgcgagccg ctcgccgagc gtttcgcccg
     2341 tcaccgtcat gcagtcgagg tggaggagcg gcttcagttc gcgcagcacc gcaccgatgc
     2401 cgcctgccgc atagaagtcc tccatgtaga actcggagcc aaccggcttc accgccgcga
     2461 gcaccggcgt cgtatccgaa agctcgttga agcggcggag atcgagcttg atcccgagcc
     2521 ggcccgcgat cgctgccaga tggatcacgc cattcgtcga accgccgagg gcgagcagta
     2581 cgcgcgtcgc gttctcgacc gactttgccg tgatgatgcg gtcgggcgtc aggttgcccg
     2641 cgatcatccg cacggccgcg gcacccgagg cctccgctgc gcgcagccga tcagcgtgca
     2701 ccgcagggat cgccgcagtc cccggcagcg tcatgccgag cgtctcggcg attgcggcca
     2761 tggtggaggc cgtccccatc accgcgcacg agccggctgt cgtcacgagc cggccctcga
     2821 cagcctcgat ctccgcctgg ctgatccggc cggcgcgata ctcgacccag aatttccggc
     2881 agtccgtgca ggcgccgacg cgatgctcac ggtagcggcc cgtcatctgc ggtccgccga
     2941 tgagttgtat tgccggtttt cctgcgctcg ccgcccccat cagttgtgcc ggtacggtct
     3001 tgtcgcaggc gccgagcatc acgacagcat ccatcggctg ggcgcggatc atctcctcgg
     3061 tgtccatcgc catgaggttg cggaacttca tggagtttgg cgcgagcgtc gattcgtgca
     3121 ccgagatggt cgggaactcg aacggcagcc cgcccgccgc cgtgatgccg cgcttcacag
     3181 cctcgacgag ctcggggaag tggcgatggc aggcattgaa gcggctgtag gtgtcggcaa
     3241 ttccgatgat cggccgcttc agttcgtcgt gcgagtagcc catcgacttg gcaaaggcgc
     3301 ggcgcagata gatcgagaaa tccgggtcgc cgtagttctg gaggccgcgc agaaagccgc
     3361 gctcggcacg tggctttact cgcgcacgct tctcctcggc gccccccttg gccccagacc
     3421 cgctcctcga ccggcctttg tcactgccct tccctgccat gcgccctgct cctcgcgatc
     3481 cacatctctg agctaagcag gtttgcgccc agaggccaat cgctgcgacg ccgcctgcag
     3541 ctcggcgaaa ggtcgacgag ccgggtacga agcgaggccg cgatcacctc atcgggtgat
     3601 gcgacctcgc tccgacctgc ggcgcgggga ggcttgcgca cgccgcaggg ttcgccagag
     3661 ggcaaaattg aacgtcacga tgagggcgaa agtcgccagg atcagcatca ttaccgagcg
     3721 ctgataggac gtcgcgagtg agagttcccc tgatatgagt tcgtgcccgg caaacaaggc
     3781 cgcgaggatc agggccnnnn nnnnnnnnng tgcgcccgat cccgcgcaga accttctggt
     3841 tggagatcgg cgctcgctcg accctgcgca gccggttgac gagttcccgc atgatgacgt
     3901 ccttcaacga ttgacctcga ttgacgtcca tctacgactt acgtcttggg cgaccgcggg
     3961 ccgtaaatgt gaccgtccgg cgtcagaggc ggtgccggcg cgcaatcatg ccagtttgtt
     4021 gcgcccatgc atcacttcgc ccccgtgctc gaggcgaagc atcactcgaa cgcgctccgc
     4081 gcacacctat tcgcgacctg gaattcgtaa taatatcagc tctgtcatga cgcatcctgt
     4141 taaacgactt ctcgccacgc tccgcggtcg gcgctcgcca catactgctg gcgccgcccc
     4201 gcactgggtt gtcgccgcca tggatccgtc ggcggcgagc taggacgtcg ccgtagcact
     4261 cgctcgccgt agtgcggctt ccatcgcccc cctccaactt ccccagctca ccgagggcgg
     4321 ttatcggctg gcgcgcgcgc tccgcaaccg gccgctggcc gcccgcgact tcgcatccgc
     4381 cgacgccctc cagcgctccg ggcatcgccg cggcgatgtc gccctcctcg tcgagcagac
     4441 tgcgaggagc gagatcgccg tcgaggtgca gccgctaact ccgctcggta tcgcgcgtgg
     4501 gctcctcgcg ccgccgatcc tgcggctgac gggcctcccc ttgagacagc ccgcaccggt
     4561 cctcgcgctg atcgctctct cgaccgctgc tcagttgctc tccatgatcg cggagcccgg
     4621 tgaacccgcg cccgccctcg acgaaatcga gcgccatctc gcgcgcctcg aggccgcgca
     4681 catgacgtca gacgcgacgc ttcgagcggc ggccttcgat ctgaagacat gggtgctcgc
     4741 ctggcgcggg cttgccgatg gtgatgtcgt gcggctcgag gacgcgcgga cccgcctcgt
     4801 cacaatgcag gcgcgagctt gcgacacctg gtccggatgc aaccgctcgg gcatgttggc
     4861 gctcgtgagc ctcgcgcttg gcggcgaggt gggcggagcc gagcgtctcg ctgaagccga
     4921 gttggccgca agcgaagcgc tctccctcgc aatcgagctg tcggagccgg caatcatcgc
     4981 gacttgctgc gccttggccg gtcgcacgtc gctggcactc ggccgtgcgc ggactgacgt
     5041 ccggcttctc gaaagtgcac gtgtccgttt cgacgaagcg cagcgccttt ccgccggggc
     5101 ctggagcgac ggcgcctgcg ggctcggcga catcgccgct gagatcggag acgtcgaagc
     5161 cgcgatccag gagttggacg cgccgctcaa gcggaccgcg gccccgctca cgcgggaccc
     5221 gccgacgctg tcgctggtcc gattgacgtc gcgcagctga tgccgctcag gctagtcggc
     5281 cgagcaggaa cgccgaggca agcgcgacca ccatgatgac cccgatcacg atccaatccg
     5341 tgctggtcgc ggatttcgcc ttcatggggc ccgccgccgc cgatctgcgc cgcaaggcct
     5401 cggacgtgat ggcagcgtcg acggccgcac ggttctcgat ctgctgcgct ttggttggct
     5461 ctgccatgga acgtgctcgc cgattgatca cggagcggaa ggctctcgcc tccgcgatca
     5521 catcgaagcg tgagaggtgc cgccagtcaa gccgatagcg tcagaccatt cggccaatca
     5581 agtgtcgcct tcaggcagtg ccgcaatcat ccgagggcgc tcccaaacgc gattggctcc
     5641 gtttgctcaa cagagccagc acaggcgccg caatccgctc ccgatcgtag cgatgctacc
     5701 aggcccccaa ctgagagctg cgaacaacaa cccagtcggc accagaaccc accgcaagat
     5761 tggcctggga ggggcgtgcg cgtttaccat catgccttgc ttcagccggg gtacgagccc
     5821 caccagaccc gtcgtcgata cggcaagcaa tccagcgctc gaaagtccgg caactccgct
     5881 gaccgtcgta aagcctccga catgtgcgag gcccgcgacg atcatcagca gcatcgcgat
     5941 cgcggttatc cgactgatcg gcccgtgctt ctgcctgtcc gacaccttcc tcgggtcgga
     6001 aatcttggtc cacgcccaca cggctgccca gacgacaacc gtcagaattg ccgtcgcgat
     6061 gacgaacgca atgacgaatg ccagccactt cggaagcgtc attggaagag ctcccacttg
     6121 tttatctggc gtgtgatcca gaccaaatgt agccaggaca gggtggatgt tgccacaacg
     6181 gaaaacgcgg taaacctgat atatgtcatg acatcgccgc gacccacagc caatttggcc
     6241 tcgcgctggc atgtcagagg aacaactgcg ctaaaaatgg tggcggtggc cagcacgaag
     6301 gcgaccgtgc ggtggtcgac accgaatgtg atgtggatca accccagatc gaagcagctc
     6361 ggattgattg caccaagcac gacgaacacg tccgcgctga cgagtgccgc cagcgctccg
     6421 acaccggaat cgctacccgg atccgcagcg agcttggcca gccagatcac gccgagccca
     6481 aacaagctcg ctgcataggc catgagcatc agccacagca aatcgattgg cgatgttata
     6541 cttgccaaac ccggcagaga gggctagctt atgcaacagc tagcgcccag cccgtcgcag
     6601 caggaatggc cgacacacgg gctcgtgagc ctgcgggata tgttcgagat ccgagcgcgg
     6661 ctttttattg cggcaagtca agcgcttgct aagctatctg ccagagttga agagcggcag
     6721 aggctatccg gggtgcatag cagtgcggat ggcgttccac gagaccctat ggccaagatc
     6781 agtgtcaccg gcccccttgc cgagatatcc gcctcttgcg aggggtttgg cggagttgtc
     6841 acaaaacagc tggcggaccg actgatcgac ttggtgtcgg cggaacttct cagcttcagc
     6901 aagttggcgg tagagataga cgccctcaat caagcctttc ttgcggaatt aaacgcaaca
     6961 aaattttatg cgataacgcc ggccatggcc aaattagtag aacggcccca agagcgcttc
     7021 tccgacgatg tccttcgagt ttttcccgag gcagcctctg acatcgatga agcggggcgc
     7081 tgctatgcgt ttggccgcca caccgcttgc gtgtttcatt tgatgagagt tcttgaggtt
     7141 cctcttcaac atcttgccaa aacgctattg cccaacgacc ggaagccgaa ctgggcacca
     7201 ataatagaaa aaatagacac tgaattgaag cttccataca aacagcggtc aattattggt
     7261 cccgaaagtt tttgggcaga cgtatctgca agaatgcacg ccgtaaaaat agcttggaga
     7321 aatagagtaa tgcacgtgga ctcgattgtg acagcagagc gcgcgagttc tattttcgac
     7381 gcaacctgtg ggcttatgaa ttttctgtcg gatcacgccg aacatttttt gcacgtggat
     7441 cagccacctt cttgaaagcc cgttcggcag cttcaggatc gacaatggaa cgtgacgcaa
     7501 catctacgaa tctcttgaac tgctccgcgg gtctctcaga aggggatttc ttcttgggca
     7561 tcggtcactt ccccacgagt cggtcgatag gtcagacgct tgccgacgac gcccttgacg
     7621 gcgcgggccg cccgctcgct gtcgtcaacg ccatgcgcga cgcgattgtt ataccggaaa
     7681 tcgaactcac tgacatagcg gtgaaggtgc tgctccccac agtgctggta gacgcccttc
     7741 atgccgcgct tgaagatcga gtagtagttc tcaagggtgt tggtgtgggc atcgccgcga
     7801 acgtactccc ccgccccgtg gttcacggtc tcatgacccg cgaactcccg gccgacacgg
     7861 cggtagtact tcgcctcatc ggtcatgagg cgcgcttcct tggcgatgtt ctcgttgacg
     7921 atcggcgcga tggtctccgc atcggccttc tcgacgtgga agctgcggac ctcgccgcca
     7981 cgctccacga gcgagagaac ggcgtgcttg tggtgatagg cgcgccgctt gggctggccc
     8041 ttcttgcggc cgatgaaggt ctcatccgcc tcgaccacgc cgctcgatcc gttgccaccc
     8101 ataggtgcgg caagggtgcc ggtccgcatc gcttcgcgga tacggtgcga catgaaccac
     8161 gcggtcttga gggtcacgcc caaggtgcgg tggagctggt tcgacgaaat gcccttcttg
     8221 ctcccggcga gaaggtagaa cgcctgaagc cagagatgca tgggaacgtg gctcgacccg
     8281 aagatggtgc cgatcttcac gttgaactgc ttgcggcagg catagcactt gtagagaccg
     8341 atccgggtgg ccttcccctg catcttcgaa acgcgcttgg tttcgccgca atgagcgcaa
     8401 acggggccat tcggccagat gtgagcctcg acccactgat aggccgcttc ctcatcctga
     8461 aggtgctttt gagagagcaa cgacatggcg aatcatctcc gatctgatgc cactatagcc
     8521 gatcgaagtg ggtttggcaa gtataacatc gccaatcgat tacatcggcc acaatccaac
     8581 ccccgcccaa acgtggcgga aagtctgggt ggaaacggat agattggtca acgcaaaacc
     8641 ggctggcgac cggcggcccc tcacttcacc gcggcggtca cgctcgcggg caggtagcgt
     8701 gcggtctcgg tccagatgcg ctcggttgcc ccggttgccc agagatagcc cgtcgcaacg
     8761 ccgatcagga tcatcgcaaa atagaacacg gggcgatagc gcctgagagc caccagcgcg
     8821 aacagcgccg caggcacgaa cacgacgagg gctccgagaa tcttgggcgt cagcatgcga
     8881 ttggttcctc gaaattccgg cagcgatgaa aactagtgca gcaacacggc agctattcgg
     8941 cggccttggc tttgcctgcg agtgcctggg atgccgcctc gaggcgggct tcctgctcct
     9001 tcagcacctg cagcgtcggc atcgagacga cattgtagcc gcagtccacg taatggacct
     9061 cgcccgtcac ggcgcctgac aagcccgaca gcagataaag cgccgcgccg ccgatctcgt
     9121 cgagatgggg cgtgcgcttc aagggcgcgt tgtcgccctg gaagttgaag atgatccgcg
     9181 catccgagat cccggcgccg gcgagcgtgc gcatggggcc ggccgagagc gcgttcacgc
     9241 ggatgccttg ctggccgaaa tccgccgcaa gatagcgcac actggcctca agtgccgcct
     9301 tggcaacccc catgacgttg taggacggca caacacgcgt cgctccgccg taggtcagcg
     9361 tcagcatcga gccgccgttc ttcatcagtg gcgccgtacg ccgcgcgatc tccgtgaacg
     9421 agaagcagga gatcaccatc gtgttggtga agttgtcccg ggtcgtgtcc gagtagcggc
     9481 cgtgcaattc gcgccgatcc gagaaggcga gcgcgtgcac cacgaaatcg aggctgcccc
     9541 acttgcggcc gatttctgcg cagacggcat ccacgctcgc gaggtcggtg acgtcgcacg
     9601 actggatgat ctcggagcct accgactcgg cgagcggaat ggtgcgccgg ccgaaggcgt
     9661 cgccctgata ggtgaacgcg agcttggcgc cgtgggccgc ggctgcgcga gcgatacccc
     9721 aggcgatcga gcggtcgttg gccacaccca tgatcaggcc gcgcttgccg gcgagcaatg
     9781 gaccaggcgt cagaatatcg nnnnnnnnnn catggcttcc tcgtcatgta ctccgtccaa
     9841 cagcgcgtcg ctgccacgac gtaacgtcga tggtgtgcca cccatcgcgc gaacgccgag
     9901 cgtagcggac attccgcccg ctgcattgga catacggcgt cacgacgaca ggcggcagcc
     9961 cgtcacccgt catggcgctt gaacaccaaa gtcgcattcg tgccgccgaa gccgaagctg
    10021 ttcgagagca cacagttaat tgcggcgccg tctatccgct tccgggcgat cggcacgtcc
    10081 gcaaaagccg gatcgagttc ctcgatattg gcactctcgc agacgaagcc gttgttgagc
    10141 atgaggaggg aaaagatcgc ctcctgcacg ccgatcgcac cgagcgaatg gccggtcagc
    10201 gacttggttg cggagatcgt cgggatctcg cccttgaaca cggccctgat ggcctcgatc
    10261 tcgcgctgat cgccgatcgg cgtgccggtg ccgtgcggat tgatgtaatc gattttcggg
    10321 gtgcccttac cgtcaactcc cttcaaggcg agctgcatgc agcgcaccgc cccctcgccc
    10381 gacggcgcca ccatgtcaaa gccgtccgag gtcgcgccat agccgcacag ttcgccgtag
    10441 atcttcgcgc cgcgcgcctt cgcccgctga tactcctcga ggacgacgac gcccgccccg
    10501 ccagcgatca cgaagccgtc gcggttcttg tcgtaggcgc ggctcgcctt ggtcggcgtc
    10561 tcgttgaaac gcgacgacat cgcccccatc gcgtcgaaga gcgaagagag cgtccactcg
    10621 agttcttcgc agccgcccgc gaacatgacg tcctgcttgc cccactggat gagttcggca
    10681 gcattgccga tgcagtgggc gctcgtcgcg caggccgacg agatcgaata attcgtcccc
    10741 ttgacctgaa aggcggtcgc gagcaccgcc gaggggcccg agcacatcgc cttgggcacc
    10801 tcgaacggcc ccatcttctt cggaccgcca gactggcgca ccgtatcggc cgccgagacg
    10861 atcgcatagg tcgagggccc gcccgagccc atgatgatgc cagtgcgctc gttcgagacc
    10921 tccgtcgctc cgaggcccgc gtcgcggatc gcctggtcca tggcgatata gttccaggcg
    10981 aggcccgact ccatgaatcg cttcggcttt cgcggcaccg cggcggacca atcgagtttc
    11041 ggctcgccgt gcacctggca cttgaaacca agctcggcgt acttctcggc gcgggctatc
    11101 ccggaccgcc cctcgcgcag cgatgccatc acctcctgcg tgctgttgcc gatggaggag
    11161 acgataccca tcccggtgac gacgacgcgt ctcattgcat agcccctgct gatgtgtacg
    11221 acgcgacccc gcgctggctt tgcgccagca tgtccgatca ggcgccgacg ccggtgtcct
    11281 cgccttgcga gaacaggccg accttgagat cggtcgcctc gtagatgacc ttgccgtcgg
    11341 ccttcataac gccgtcgccg ataccgagct tcaggcggcg gaggatgact cgcttcaggt
    11401 cgacgacgta ctcgagccgg ccgacgctcg gcagcaccat gcccgtgaac tttacctcgc
    11461 cgacgccgag cgcgcgccct cgtccgggcg caccgagcca gccgaggtag aagcccgtga
    11521 gttgccagag cgcgtcgagg ccgaggcagc caggcataac gggatcgccc ttgaagtggc
    11581 acgcaaaaaa ccagtcgaga gcgggatttc cggcaacctt cagctcggcg acgatctggc
    11641 ctttgccgtg cgcgccgccg gcctcgttca cctggctgat gcggtcgaac atcagcatcg
    11701 gcggcaacgg cagctgcgca ttcccggcgc cgaacagctc tccgcgtccg caggcgagca
    11761 aatcctggta atcgaagctc gagcgacgct ctgccatggt ttgggccggt ctccgccgaa
    11821 tgcatccgag cgaccgagcc acgcgcggcc gttgttcctc gcgcgttggt aacacactcg
    11881 atcgtcgttt caaacggtcg cgtttaccct gttggcggac cgagcaccgc ggctcgtgat
    11941 cgccgcgcgc cgtgcgttgg atcaatcaga ccggacgacg agacaatcga tctgcagccg
    12001 gcgcagttcg aggcacgtcg atgcggccgt ggcagcatcg aagcccgcga agcgcgcacg
    12061 cacgaaggcc tttccggccg actgcacggg ctgtgtatag gtcnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnccttcgac acggcgagct gcagctggcg ttgcgcttcg gcctcgcttg
    12181 cataggcgcc gatctggatt gccatgccgc cggcggtgga cctcttggct gccggggctg
    12241 cgggcagcgg tcccttcagg ttgtaggtcg gcggaggcgg cggaggcgcg gcgacgtggg
    12301 tcgcgggagc acctcgcgcc atgcgcgcgg cctgatccga gagagtcgag ggcgccaagc
    12361 cgggcgccgc gtccgaggtg gcactggctg ctgctacgcg cttcggcact tcgcggcggg
    12421 gacctggcac ggccgcggcc ggtgggggga tcg
//
LOCUS       DAORNH010000119        12450 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3839_length_12450_cov_2.679710, whole genome shotgun sequence.
ACCESSION   DAORNH010000119 DAORNH010000000
VERSION     DAORNH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12450
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3839_length_12450_cov_2.679710"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    137..167
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     repeat_region   168..591
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=168..195
                     /rpt_unit_seq="cggcccgcgcggcgatctctgctagaac"
     gene            complement(707..1048)
                     /gene="cas2"
                     /locus_tag="PK264_12975"
     CDS             complement(707..1048)
                     /gene="cas2"
                     /locus_tag="PK264_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRD76826.1"
                     /translation="MAQFTALSGYRIMWVMALFDLPVLTKEERSRATKFREYLLDQGF
                     VMMQLSCYIKFTPGKEKADALAAKVGAEVPPGGKVDILFFTDAQYGAIKSFRGKGCSA
                     RPGKPDQLQLF"
     gene            complement(1048..1980)
                     /gene="cas1"
                     /locus_tag="PK264_12980"
     CDS             complement(1048..1980)
                     /gene="cas1"
                     /locus_tag="PK264_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HRD76827.1"
                     /translation="MLGRIVEISGQGRRLSLHRGFLEISGPEGILGQIPLDDIEAVIA
                     STPAVSYTSQALTALAERGAPVVICGPNFTPSAFLLPVDGHHAQGERFEAQAGARLPV
                     RKRLWAEIVKAKVSAQAAALERVGANPLPVRHLVSRIRSGDIGNVEAQAAQKYLPAMF
                     GATFKRDREADGVNAALNYGYTVLRAATARSIVAAGLHPSLGLHHVSKGSSLRLADDL
                     MEPFRPAVDLLVLGMARHGAIGLDPPTKRTLAGVLHADYPTAEGASPLSNALTRLAQS
                     LAQVFLGERRYLAWPLELVPLAPTALAVSDAGGA"
     gene            complement(2477..5758)
                     /gene="cas9"
                     /locus_tag="PK264_12985"
     CDS             complement(2477..5758)
                     /gene="cas9"
                     /locus_tag="PK264_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRD76828.1"
                     /translation="MQRWLRYAFDLGTNSIGWAVYRLSGDPATSSTTVCELLGAGVRV
                     MSQGVAEAGRDVKGASLAEARRVPRSARKRRDRFVMRRATLIKQLCTLGLLPGDHEAR
                     RTLADLDPYRLRAEGLDRRLEPHEIGRALLHINQRRGFRSNRRADRKAKDDDKGKIAT
                     GVNRLSSELAVSRSRTFGEYLWRRHGGTDGKATPRTRQAVRIRMEGEGTKSLYAVYPS
                     RQMLKEEFDSLLAAQTTHHSELLKPEVIADIRETIFHQRPLKPVEVGRCTFVPEERRL
                     PKALPSVEARVIYETLNALRFGTGLKLEQRLSTEHRDALAVTLMRGENVTFRQLRKKI
                     GVRDDVRISLEEGGTKDMDDYRACSAALAWRKLKGKERQELFGERWFAMSLVERDEIV
                     NCLIDAEDEKEVVDWLTSGYKLSDSAARGVAAWMPPDGHARLGRTANDAVLAELKASD
                     LPTYAEAVKRAGWHHSDERDGVIELPLPYYGQVLERHVLFGSGKPEQDPIQRYGRFPN
                     PTAHIALNQLRRIINLLARTYGAPSQIVLELARDLKMNKEQKDEAKKRNRDNRTIRDR
                     HRAILTEHHQHETADNFLRLRLFEEQERAGGGVARCPFSLKPIEIAQLFSPEVEIEHV
                     LPWSRTFDDSAANKVVCFREVNRSKGKRTPHSAFGDDTRWSDIAANALALPRNKRWRF
                     APDAMERFENEERNFLARQLNETRHLSRMARIYLSRACDPDRIYVTTGQMTAMLRRRW
                     GLNSLFRDHNRRPDEAGEEAGTLTAKARDDHRHHAVDACVIGAIDRSLLQEMSRRAGQ
                     AETEGRERVVMEAADEAGPFPGFRDAVRAAIEKVVVSIKPEHGKGGALHEETAYGLVT
                     NEVEAKEIGNLVYRKLLKDLTAAEINRIRDVRLRAEVQAATANTRNDEKALAAALAQF
                     ADQKDQPGREKGVRRVRIGKVKAGEVPIRDRRTGAVYKALLPGENHHIDIVQMREGSW
                     KGFAATVFEVNQKGWRPRWERDKLGGKLVMRLHKGDAIEVDDPDGVRRIKTVHRIEIS
                     ANRVRLATHHEGGKLGDRHDNKDDPFRWDFANIPGLRDRRARKVKVDAVGRVHPARSN
                     TQNSR"
     gene            6516..7073
                     /locus_tag="PK264_12990"
     CDS             6516..7073
                     /locus_tag="PK264_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76829.1"
                     /translation="MSPLRFQWTGDGIARITTAVETLAGSRKEAALRRAINHTGDKTL
                     TRVRRTLAKQMGLASQKLLSRGRTLVPRRATGSHLEYQIVSRGKAVRAKEFRHRVGKK
                     GITFYPWGVAHNIRSAFVIGRWGGNFYRRRTSARFPLEALYGPNINKELVKDATAAAF
                     FSIVARDLPARVEHEVRVITNGIVS"
     gene            7203..7712
                     /locus_tag="PK264_12995"
     CDS             7203..7712
                     /locus_tag="PK264_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76830.1"
                     /translation="MAKNAVREEISVKRLAEVIGVGERWLQQLETQGVIAKTRHGRYL
                     LAESVQAYIKFKVDSEVARVTPSETNAGERVKEERARKLKLENDEKELRLVQTPDAVA
                     ALDAIVGPLVADLAGVPAKVTSDVAERRRIEDAIDTVLRGLADRFAKAGSDLRAGRDP
                     LAADDEDDA"
     gene            7750..>9583
                     /locus_tag="PK264_13000"
     CDS             7750..>9583
                     /locus_tag="PK264_13000"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRD76831.1"
                     /translation="MDRPGPRDPNLTPYVIAFERAFDAVAYEIACLVCGAQMGKSETA
                     LDVIGHRFDQRPTPTLYVGPSQDFIVDEIEPRVMELINSAASLARKLSRGKKNKRFRK
                     VIGGVPLKFAWAGSATQLSGTTAGLVMVDEYDRMMEDVAGEGDPLELTKARGFTFRDR
                     KYGVTSTPKKGTVDIEADAASGLEFWKRMPPEDIESPVWKLWQSGTMFHFAWPCPECG
                     EFFIPRFKQLAWPKDATPDDALRTAHCTCPRCGGVIEERHKAEMNARGVYVAPGQSVT
                     PDGIVIGEPPSSTTASFWVSGLCSPFVTFGERARDYLAAKASGDARKLQTVINTGFGE
                     LWAEGGGDAPEVAEVARLRQPYRLGDLPAGTVHLTTGVDVQKNRLVYSVRAWGPRATS
                     WLVTRGEMHGETHEEEVWGDLADLLETPIHGHLIRRALIDSGFRPGKPFLVPVNRVYE
                     FCRRHSRVASPSKGRATQAVPVRKSSIEVTAKGDKRKYSLDLMLIDTDWAKSSVHEHM
                     RYPIDALGAWLLPEDIDDAYIASMLSESRVRRPNGRHAWVQRSRENHFFDCEALNWAA
                     GHLLNVQRIREGRGLKTAAVAETVEDAPLADDAVDVEVKPPQQPP"
     assembly_gap    9584..9783
                     /estimated_length=200
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9784..11380
                     /locus_tag="PK264_13005"
     CDS             <9784..11380
                     /locus_tag="PK264_13005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRD76832.1"
                     /translation="GLGQRLTSFARSLMPTRGVPSRPSPKAGFMRDGSAGLLSAWRPT
                     LRDSHDDVRQAWGMAAARAIDSLHNSGWLAGAIEQCSADTIGDGLRLNATPDIDALGI
                     SEDEAHELCRRIERRWRAWSRNPLECDARGKRTIGDMTDAAIRHHYAFGEAVALIPRI
                     ARPSSTSSTKVLMVSPHRLKQDSDERLGLITGVQLDGNGMPTAYRFSRRVAGFEQDLD
                     LKARARDGRPQVVHVFDGAPDQVRGITPLAPVLKVLRQYDQLADATLTTALIQTIIAA
                     TIKSPAFDGQAFEGFSAVDSELPDEVAGSLSARASWWDGRKLDLGAHGKIAHLFPGEE
                     FGLHAAKTPGDQYLPFSKNLLREVARCIGTTYEAMTGDYEGATYSSVRMGTSSIWFVT
                     LRRRVRIGVPFVQPIYAAWLDEEVSTGRIAWPGDYQAFAANRAAATQAEWFGPAKPTA
                     DDLKTAKAQSERLANGTTSLAFECAEYGLDPETVMEQRQQDRRMAAKFGLPDPYQPRG
                     GRAHEDDHDDGDEPLVKSKRRLS"
     gene            11380..11649
                     /locus_tag="PK264_13010"
     CDS             11380..11649
                     /locus_tag="PK264_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76833.1"
                     /translation="MAVDWDALDYDDPCALLTKLRPAYYRLLAGDAEEEIEGTDRRRV
                     RFQRSDLKGLAAVIADLEAKCAAKSGKRRRFAMSGQARPRKDTYS"
     gene            11646..>12450
                     /locus_tag="PK264_13015"
     CDS             11646..>12450
                     /locus_tag="PK264_13015"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRD76834.1"
                     /translation="MTTLPRIASLVFNRPHYITRDAGLAIANAVTRGGLGSLAPEASR
                     FVGEEQVERDEDGRPITDAWGNKIRRGFRRQGSTAIVNVLGELVNRGAWLDARSGLVS
                     YEGFAQQLRAVSSEVAAGRVRALILDVNSPGGEAAGMTDAADLVRALAAEIPVVAVAN
                     SMMCSAAYGICSGATRIVTTELGVVGSIGVVMMHADYSGALGKSGIDVTLIHEGAHKV
                     DGNPFEALPADVRDAFQAECAKYYAAFVGCVAAGRRSLSPAGIRATEART"
BASE COUNT         2098 a         4056 c         3945 g         2120 t
ORIGIN      
        1 ccggctagcc gatcgaatca cgccgcaagg ttccggtcgg cccgcgcggc gatctctgct
       61 agaacccaga tcataccgac atgcacggcg ccgctgttcc ggtcggcccg cgcggcgatc
      121 tctgctagaa cttgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg cccgcgcggc
      181 gatctctgct agaaccttcg acgagatcag gcggctcggc ggatcgttcc ggtcggcccg
      241 cgcggcgatc tctgctagaa ccctaccccg tcccaccccg ccaccgccgg cgttccggtc
      301 ggcccgcgcg gcgatctctg ctagaaccgc cagggcgagc accccgagga tcacgttgtt
      361 ccggtcggcc cgcgcggcga tctctgctag aactgcgcgc ggcggggctg ccatgagcaa
      421 tgagttccgg tcggcccgcg cggcgatctc tgctagaacc gccgacaagg cagttcaggg
      481 cctcgtctag ttccggtcgg cccgcgcggc gatctctgct agaaccgtca cctggtacta
      541 cctcggcgag ctcaagttcc ggtcggcccg cgcggcgatc tctgctagaa cagatcctcg
      601 gcaaccttct ggtttccgag ggcttttagt gcctggccgc cgaaaaaaag gaccctgcgg
      661 tttccggtca ggagaaccga agcaccattt ttcacgagag gtcgcctcag aagagttgca
      721 actggtctgg cttacccggc cgcgccgagc atcctttgcc gcggaaagac ttgatggccc
      781 cgtactgagc atcagtgaag aagagaatat cgaccttgcc gccaggcggc acctcggctc
      841 ctaccttagc cgcaagcgca tcggccttct ccttgccggg cgtgaacttg atgtagcagg
      901 agagctgcat catcacaaag ccctggtcga gcagatattc ccggaacttt gtggcgcgcg
      961 agcgctcctc cttggtcagc accggcaaat cgaacagcgc catcacccac atgatgcgat
     1021 accccgaaag cgcggtgaac tgagccatta cgcaccgccc gcgtcgctga ccgcgagagc
     1081 cgtcggggcg agcggaacca actcgagcgg ccaggcgaga tatcgccgct cacccaaaaa
     1141 gacctgggcc agcgactgtg ccagccgcgt cagcgcgttg gacagcgggg aggctccctc
     1201 cgccgtcggg taatcggcat ggaggacccc ggcgagcgtg cgtttagtcg gtgggtcgag
     1261 cccgatggcg ccgtgcctag ccatgccgag cacgagaagg tcgacggcgg gcctgaacgg
     1321 ctccatcagg tcgtcggcga gcctgaggct cgagcccttg ctgacgtggt gcaggccgag
     1381 cgaaggatgc aagcccgcgg ccacgatgga gcgcgctgta gctgcccgca gcactgtgta
     1441 gccatagttg agggcggcgt tgacgccgtc agcctcccga tccctcttga aggtcgcgcc
     1501 gaacatcgcc ggcagatatt tctgggccgc ctgcgcctcg acgttaccaa tatcgccgga
     1561 gcgaatgcgc gagacgagat ggcggaccgg aagaggattt gcgccgacgc gctcgagcgc
     1621 cgctgcctgg gctgagacct tggccttcac gatctcggcc cataggcgtt tgcggacggg
     1681 gagccgggcg ccggcctggg cctcgaagcg ctcgccctgg gcgtgatggc cgtcgaccgg
     1741 gagcagaaag gcagaaggtg tgaagttggg accgcagatg acgacgggcg cgccgcgctc
     1801 tgcgagagcc gtcaacgcct ggctcgtata ggagacggcc ggcgtcgagg cgatgaccgc
     1861 ctcgatgtcg tcgagcggaa tttgtcccag aatgccctcg ggccccgaga tctccaggaa
     1921 gccgcggtgg agcgagagcc ggcggccctg acccgatatc tcgacgatcc gccccagcat
     1981 gggctgctcc tgctcgccgg tctaggagta gtggagctgg aacagtgaaa gaacgccagg
     2041 ttttgtagtg ggcaaggtga acaggcggag ggatgtcctc cggctgccgg tggcccgttg
     2101 agggccggtt ctagcggaga tcacaagtgg tccaaccgga acacccgcct atgcacacat
     2161 tagacatggg aatcacgggc aggtagatgc ggcgaccctg acgcgtatgc aatgaggatc
     2221 catacaaccg cggcgcctcc gtaaggggtc atccggttgc ctctagcgat tggagtggtc
     2281 gtggtcggat atcctgatac ttgccatgtg ccggattccg gacacgactc tcctgttcgg
     2341 gcggttgtcc cgatgggtga tcttggcagc tttctcacct cgttttcaca gctataggag
     2401 ggtaggcaca gcgccggccc ttatcgcgca gttctgattc ctgctcctag aaggaagcaa
     2461 gagctgtgca ccactattaa cgtgaattct gtgtattact tcgggcagga tgtacgcgac
     2521 ccacggcatc gactttcacc ttcctggccc gacggtctct caggccagga atattggcga
     2581 agtcccagcg gaaagggtcg tccttattgt cgtgccgatc gccgagcttt ccgccttcat
     2641 gatgtgtggc gaggcggacg cgattggcgg aaatctcgat gcgatgcacg gtcttgatcc
     2701 ggcgtacgcc gtccggatcg tcgacctcga tcgcatcacc cttgtgcagg cgcatgacca
     2761 gcttgccccc gagcttgtcc ctctcccatc ggggtctcca gcccttctga ttgacctcga
     2821 acacggtcgc cgcgaatccc ttccatgaac cttcgcgcat ctggacgatg tcgatgtggt
     2881 ggttctcgcc gggaaggagc gccttgtaaa cggcgccggt gcgccgatcg cgtatgggca
     2941 cctcgccggc cttgaccttg ccgatgcgca cgcggcggac gcccttctcg cggcccggct
     3001 gatctttctg atcggcaaat tgagcgagtg cagctgccaa ggccttttcg tcatttctgg
     3061 tattggcggt cgcggcttgc acctcggcac gcaatcggac atcgcgaatg cggttgatct
     3121 cggccgccgt gaggtccttg agaagcttcc tgtagacgag gttgccgatc tctttcgcct
     3181 cgacctcgtt agtcaccagg ccataagccg tctcctcgtg cagtgcgccg cccttgccgt
     3241 gctctggctt gatggacacg acgaccttct cgattgcggc gcgcaccgcg tcgcggaagc
     3301 ccgggaacgg cccggcctcg tcggccgctt ccatcaccac gcgctcgcgg ccctcggtct
     3361 ccgcctggcc tgcgcggcgg gacatctcct gcagcagaga tcggtcgatg gcgccgatca
     3421 cgcacgcgtc cacggcatgg tggcggtggt cgtcgcgcgc cttcgcggtc aatgttcccg
     3481 cctcctctcc tgcctcgtcg ggccggcgat tgtggtcacg gaacagtgag ttcaagcccc
     3541 agcgacggcg cagcatcgcc gtcatctggc cggtcgtgac gtagatgcgg tcggggtcgc
     3601 aggcccgcga gagatagatg cgcgccatgc gtgagagatg gcgcgtctcg ttcagctggc
     3661 gggcgaggaa attgcgctcc tcgttctcga accgctccat ggcgtcggga gcgaaccgcc
     3721 agcgcttgtt gcgcggcaat gcgagtgcat tggcggcgat gtcagaccat ctcgtgtcgt
     3781 cgccgaacgc gctgtgtggc gtccgcttgc ccttgctgcg gttcacctca cggaagcaga
     3841 cgaccttgtt ggcggcgctg tcgtcaaagg tgcgagacca gggcagaacg tgctcgatct
     3901 ccacctcggg tgagaaaagc tgggcaatct cgatcggctt caaggaaaac ggacacctgg
     3961 cgacgccccc accggcgcgc tcctgctcct cgaagagcct gaggcgcagg aaattgtcgg
     4021 ccgtttcgtg ctgatggtgc tccgtgagaa tcgcacggtg acgatcgcgg atggtgcggt
     4081 tgtcgcgatt tctcttcttt gcctcgtctt tctgctcttt gttcatcttc aggtcgcggg
     4141 ccagctcgag cacgatctgg gatggcgccc cgtaggtgcg ggcaagcaga ttgatgatgc
     4201 ggcgtagctg gttgagcgcg atgtgcgccg tgggattggg gaaacggccg tagcgctgaa
     4261 tcggatcctg ctcgggcttg ccggagccaa acaggacgtg ccgctcgagc acctggccat
     4321 aataaggcag cggcagctcg atcacgccgt cccgctcatc ggagtggtgc cagccagcac
     4381 gtttgactgc ctctgcgtag gtgggcaggt ctgacgcctt caactccgcg agcacagcgt
     4441 cattcgccgt ccggcccagc cgggcatgac cgtccggcgg catccaagca gcgactccgc
     4501 gcgctgctga atcagacagc ttgtagcccg aggtcagcca atcgacgacc tccttctcat
     4561 cctcggcatc aatcaggcaa ttgacgatct cgtcgcgctc gacgagtgac atcgcgaacc
     4621 agcgctcccc gaacagctcc tggcgctctt tccctttgag tttgcgccag gcgagcgcgg
     4681 ccgaacatgc ccggtagtcg tccatatcct tcgtgccgcc ttcctcaagt gaaatgcgga
     4741 catcgtcgcg gacgccaatt ttcttgcgga gctggcggaa cgttacgttt tcgccgcgca
     4801 tgagcgtcac ggcgagggca tcgcgatgct cggttgacaa tctctgctca agcttcaggc
     4861 ctgtgccgaa ccgaagcgca ttgagcgtct cgtagattac acgcgcttcg acagacggca
     4921 atgccttcgg caagcgccgt tcctccggca cgaatgtgca gcggccaacc tcgaccggct
     4981 tgagtggccg ttggtggaag atcgtctcgc ggatatcggc gatcacttca ggcttcaaaa
     5041 gctccgaatg atgcgtggtc tgcgcagcga gcaaggagtc gaattcctcc ttgagcatct
     5101 ggcgggaggg atagacggca tagagcgact tcgtgccctc accctccata cggatgcgca
     5161 ccgcttgtcg ggtccgcggc gttgcctttc cgtcagtgcc gccgtggcgt cgccacaaat
     5221 actcgccaaa cgtgcgcgaa cgagagactg ctagctcgga cgagagccga ttcacgcccg
     5281 tcgcgatctt gcccttgtcg tcatccttgg ccttgcggtc ggctctgcga ttggagcgga
     5341 accctcggcg ctgattgatg tgcagcagtg cccgtccaat ctcgtgaggc tcgagccggc
     5401 gatcgaggcc ttctgcgcgc agtctgtaag ggtcgaggtc ggcgagtgta cgtcgggcct
     5461 catggtcccc tggcagcaga ccgagcgtgc agagctgctt gatgagtgtc gcccgccgca
     5521 tgacgaagcg atcgcgacgt ttgcgcgccg atcgtggaac ccgtcgcgcc tcggccagtg
     5581 aagccccttt cacgtcgcgg cctgcctcgg caacgccttg gctcatcacc ctgacgccgg
     5641 cgccgagcag ttcgcaaacg gttgtgctgg aggtcgcggg atcgccgctg agccggtata
     5701 ccgcccaacc aatcgagttg gtgccgagat cgaaggcgta gcgcagccat cgttgcatgg
     5761 aaatccccgc ggtactctgg cgcaaagtga agcagatgcc ttgcagggga gcaagtagtg
     5821 cgctaccgaa tgtgcgttct agcagagatc gcggcgtggg ccctccggta acaaggagtt
     5881 ccactccacc agatcaaggg cgggctccgg tccgcccttt tttgcgtttg aaggataagc
     5941 gcggacgcaa ttccaattgt tgagcgcact ggacgtgatc agtcggagag cggaccaagg
     6001 cattacgccg cctaggatac tcggagcgcc agaaccgaat gtctccttgt gggacaactg
     6061 aactctttcg cggcacgcga cgtgttgcgc agccagagcc ttcgtcttgg cccgagaagg
     6121 catgcaccat tttacttgcc ccagaggcca atccgaaaat ttcttgttcg attggcaccg
     6181 gcagtgtctt caggttatcc gcacatgtta ttcctataag tcaggtactc tgaaagcgct
     6241 tacctgagtt cccgacctcg atggttggca atcaaacgcc cttgcaaccc ggaaccagaa
     6301 cgtcgaaagt ctgttgacag caaaccccga ctcatggcga atcgttgcca tgctcgcgag
     6361 agctgtgatc ggttggcaaa tgtcagccgt tttttgttgt tccgggggtg cggtaaggcc
     6421 gccagcgtgt cgccaacggg cgcctcgcat cgaggcaggc ccatcagtcg caatccggca
     6481 aaccaggccc gccgccccgc attccaggac catcgatgtc gccactccgg ttccaatgga
     6541 cgggcgatgg catcgcgcgc atcacaacgg ccgtcgagac gctcgccggg tcgcgcaagg
     6601 aggcggcgct ccgccgcgcc atcaatcaca ccggcgacaa gacgctgacg cgcgtgcggc
     6661 gcaccttggc gaagcagatg ggtctcgctt cgcagaagct gctctcgcga ggacgcacgc
     6721 tcgtcccgcg ccgtgccacg ggctcgcatc tcgagtacca gatcgtgtcg cgcggcaagg
     6781 cggtgcgggc caaggagttc cgtcatcgcg tcggcaagaa gggcatcacc ttctatccgt
     6841 ggggtgtggc acacaacatc cgctcggcct tcgtgattgg acgctggggt ggcaacttct
     6901 atcgccgccg cacctcggca cgcttcccgc tcgaggcgct ctatgggccg aacatcaaca
     6961 aggagctggt gaaggacgcc acggccgctg cgttcttctc gatcgtggca cgcgacctgc
     7021 ccgcccgcgt cgagcacgag gtgcgcgtca tcaccaacgg catcgtctcc tagccgtggc
     7081 cagccgcggc cagggtgtgc cgcccttagg gaccgtaccg ccccccgccc cgagcgctcg
     7141 cgcgccagcc cgggctttgt acatttttga gggccgaaaa ttgcggcttc ggactcggga
     7201 aaatggctaa gaacgccgtt cgtgaagaaa tctcggtcaa gaggctggcc gaggtgatcg
     7261 gcgtcggtga gcgctggctg caacaacttg agacgcaagg cgtaatcgcg aagacgaggc
     7321 acggcagata tctgctcgcc gagtcggtgc aagcctatat caaattcaag gtcgatagcg
     7381 aagtcgcgcg cgtgacgccg tccgagacca acgccggtga gcgggtcaag gaggagcggg
     7441 cgcgcaagct gaagctcgag aacgacgaaa aggagctgcg gctcgtgcag acgccggatg
     7501 cggtggcggc cctcgacgcc atcgtcggcc cccttgtcgc tgacctcgcc ggcgttccgg
     7561 caaaagtgac aagcgacgtt gcagagcgcc ggcgcatcga ggatgccatc gacaccgttc
     7621 tccgaggtct cgccgaccga tttgccaaag ctggctcgga tcttcgagcg ggccgcgatc
     7681 ctcttgcggc cgacgacgag gacgacgcct gatgtctggg cgcgggaaaa ccgcgtctac
     7741 ccaccgagca tggaccgccc cgggccgcgc gatccgaacc tcacacccta cgtcatcgct
     7801 ttcgagcgag ccttcgacgc cgtcgcctac gagatcgcct gcctcgtctg cggcgcgcag
     7861 atgggcaaga gcgagaccgc cctcgacgtc atcggccacc gcttcgacca acgcccgacg
     7921 ccgacactct acgtcgggcc gtcgcaagac ttcatcgtcg acgagatcga gccccgcgtc
     7981 atggagctca tcaacagcgc ggcgagcctc gcccgcaagc tctcgcgcgg caagaagaac
     8041 aagcggttca ggaaggtgat cggcggcgtc ccgctgaagt tcgcctgggc cggctcggcg
     8101 acacagctct cgggcacgac ggccggcctc gtgatggtcg acgagtacga ccgcatgatg
     8161 gaagacgtcg ccggagaagg cgatccgctc gagctcacga aggctcgcgg gttcaccttc
     8221 cgtgatcgca aatacggtgt cacctcgacg ccgaagaaag gcaccgtcga catcgaggcc
     8281 gacgccgcct cgggcttgga gttctggaaa cggatgccgc ccgaggatat cgagagcccg
     8341 gtctggaagc tctggcagtc gggcacgatg ttccacttcg cgtggccgtg tcccgagtgc
     8401 ggcgaattct tcattccgcg cttcaagcag ctcgcctggc cgaaggacgc gacaccggac
     8461 gacgccctga ggacggccca ctgcacctgc ccgcggtgcg gtggcgtcat cgaggagcgc
     8521 cacaaggccg agatgaacgc gcgcggcgtc tacgtcgctc ccggccaatc ggtgacgccc
     8581 gatggcatcg tcatcggcga gccgcccagc tcgacgacgg cgtcgttctg ggtctcgggc
     8641 ctctgctcgc ccttcgtcac cttcggcgag cgcgcccgcg actacctagc tgccaaggca
     8701 agcggcgatg cccgcaagct gcagacggtg atcaacaccg gcttcggcga actctgggcg
     8761 gagggcggtg gcgatgcccc cgaggtcgcc gaggttgcgc ggctccgcca gccctaccgg
     8821 ctcggcgacc tgcccgccgg cacggtgcac ctcactaccg gcgtcgacgt gcagaagaac
     8881 cgcctcgtct acagtgtccg cgcctggggg cctcgcgcca cgagctggct cgtcacccgc
     8941 ggcgagatgc acggggagac gcacgaggaa gaggtgtggg gcgatctcgc cgatctcctc
     9001 gagacaccga tccacggaca cctgatccgc cgcgcgctga tcgactcggg atttcgtccg
     9061 ggcaagccgt tcctcgtgcc ggtcaaccgg gtctacgaat tctgccgccg acattcccgc
     9121 gtcgcctcgc cgtccaaggg gcgcgccacg caggccgtgc cggtgcggaa atcgtcgatc
     9181 gaagtcacgg ccaagggcga caagcgcaag tattcgctcg atctcatgct catcgacacg
     9241 gattgggcca agtcgagcgt gcacgagcac atgcgctacc cgatcgatgc cctcggcgcc
     9301 tggctgctgc ccgaggacat cgacgacgcc tacatcgcat cgatgctatc ggaatcgcgc
     9361 gtgcgccggc caaacggccg gcacgcctgg gtgcagcgct cgcgcgagaa ccacttcttc
     9421 gactgcgagg ccctcaactg ggccgccggc catctgctca acgtgcagcg catccgggag
     9481 ggccgtggcc tgaagaccgc cgccgtcgcc gagacggtcg aggatgcgcc gctcgccgac
     9541 gatgccgttg acgtcgaggt caagccgcca cagcagccgc cagnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnntggcctt ggtcagcgcc tcacctcctt cgcccgctcg ctcatgccga cgcgcggcgt
     9841 cccgtcccgg ccgtcgccga aggccggctt catgcgcgac ggctcggcag gcctcctctc
     9901 cgcctggcgc ccgacactgc gtgacagcca cgacgacgtg cggcaggcgt ggggcatggc
     9961 ggcggcccgc gccatcgact cgcttcacaa ttccggctgg ctcgccggtg ccatcgagca
    10021 gtgctcggcc gacacgatcg gcgatgggct caggctcaac gcgacgcccg atatcgacgc
    10081 cctcggcatc tctgaggacg aggcgcacga actctgccgc aggatcgaga ggcgctggcg
    10141 cgcctggtcc cgcaacccgc tcgagtgtga cgcgcgcggc aagcgcacga tcggcgacat
    10201 gaccgacgcc gccatccggc atcactacgc cttcggcgaa gccgtggcgc tgatcccgcg
    10261 catcgcacgc ccctcgtcga cctcgtcgac caaggtgctg atggtctcgc cgcaccgcct
    10321 caagcaggac agcgacgagc gcctcgggct catcacgggc gtgcagctcg acggcaacgg
    10381 gatgccgacc gcctaccgct tctcgcgccg cgtcgccggc ttcgagcagg acctcgacct
    10441 caaggcgcgg gcgcgcgacg gacgcccgca ggtcgtgcac gtcttcgacg gcgcgcccga
    10501 tcaagttcgc ggcatcacgc cgctggcccc ggtcctcaag gtgcttcgcc agtacgacca
    10561 gctcgccgac gcgacgctga cgacggcgct catccagacg atcattgccg cgacgatcaa
    10621 gtcgccggcc ttcgacggtc aggccttcga aggtttctcg gcagtcgaca gcgagctgcc
    10681 cgacgaggtg gccggctcct tgtcggcgcg cgcgtcgtgg tgggacggcc gaaagctcga
    10741 tctcggcgcg catggcaaga tcgcgcacct cttcccgggc gaggagttcg gactccatgc
    10801 ggcgaagacg ccgggagatc aatacctgcc gttctcgaag aatctgctgc gcgaagtcgc
    10861 ccgctgcatc ggcacgacct acgaggccat gacgggggac tacgagggcg cgacctattc
    10921 gagcgtgcgg atggggacga gctcgatctg gttcgtgacg ctgcggcgcc gtgtacggat
    10981 tggcgtgccg ttcgtgcagc cgatctatgc agcctggctc gacgaggaag tgtcgaccgg
    11041 gcgcatcgcc tggcctggcg actatcaggc cttcgcggcc aaccgcgccg ccgcgacgca
    11101 agccgagtgg ttcggtccgg cgaagccgac ggccgacgat ctgaagacgg ccaaggcgca
    11161 gtccgagcgg ctcgccaacg ggacgacgtc gctcgccttc gagtgcgccg agtacggact
    11221 cgaccccgag accgtgatgg agcagcgcca gcaggatcgg cgcatggccg ccaagttcgg
    11281 cctgcctgac ccttatcaac cgcgcggcgg tcgagcccac gaagacgatc acgacgacgg
    11341 cgacgagccg ctcgtgaaat cgaagcggag gctcagctga tggccgtcga ttgggacgcg
    11401 ctcgactacg acgatccgtg cgcgctgctc acgaagctcc ggccagccta ctaccgcctg
    11461 ctcgccggtg atgccgagga ggagatcgag ggcacggacc gacgccgcgt gcgcttccag
    11521 agaagtgatc tcaaaggcct cgccgcggtg atcgccgatc tcgaggcgaa gtgcgcggcc
    11581 aagtcgggaa agcgccgccg gttcgccatg tccggccagg cccggccgcg taaggacacc
    11641 tactcatgac gaccctgccg cgcattgcga gcctcgtctt caatcgcccg cactacatca
    11701 cgcgcgatgc cggcctcgcc atcgcgaatg ccgtgacacg cggcgggctc ggatcgttgg
    11761 cacccgaggc gagccgcttc gtcggcgagg agcaggtcga gcgcgacgag gacggtcgtc
    11821 cgatcacgga tgcctggggc aacaagatca ggcgcggctt ccgccgacag ggctcgacgg
    11881 ccatcgtcaa cgtgctcggc gagctcgtca atcgcggcgc ctggctcgac gcgcgctcgg
    11941 gcctcgtctc ctacgaaggg ttcgcgcaac agctgcgcgc cgtcagctcc gaggtggctg
    12001 ccggccgcgt ccgggctctg atcctcgacg tcaattcgcc gggcggcgag gccgccggca
    12061 tgacggatgc tgcggacctg gtcagggcgc tcgccgccga gatccccgtc gtcgccgtgg
    12121 ccaacagcat gatgtgctcg gccgcctacg gcatctgctc gggcgctacc cgcatcgtca
    12181 cgaccgagct cggcgtggtc ggctcgattg gtgtcgtcat gatgcatgcc gactattcgg
    12241 gagcgctcgg caaatccggc atcgacgtca cgctgatcca cgaaggtgcc cacaaggtgg
    12301 acggcaaccc gttcgaggcg ctgccggccg acgtgcgcga cgccttccag gcggagtgcg
    12361 ccaaatacta cgcggccttc gtcggctgcg tcgccgccgg gcgccgctcg ctctcgcccg
    12421 ccggcatccg cgccacggag gcgcgcacct
//
LOCUS       DAORNH010000120        12264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3902_length_12264_cov_2.683266, whole genome shotgun sequence.
ACCESSION   DAORNH010000120 DAORNH010000000
VERSION     DAORNH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12264
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_12264_cov_2.683266"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1042
                     /locus_tag="PK264_13020"
     CDS             131..1042
                     /locus_tag="PK264_13020"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76835.1"
                     /translation="MTATSSAIGFVGLGVMGEAMCRNLATKSKARVIAYDRRAEPLER
                     LAAVGVTRAGSLAALAADADTILMCLPGGPEVEAVALGKDGLVAHARRGQTLVDMSTA
                     PPRLMREIERQLVPRGARFADAPVARTRQAAVDGTLSIMVGGAADLFAELKPILATMG
                     SEVTHCGAVGAGQIVKLMNNMVVFETTMALAEAISIAEASGVDGKLLFETMAKGSADS
                     FVLRNHGMKNLVPQSYPEQAFSVRYAQKDLSYARDLAREAGVDARAADLVARLFDEAI
                     GRGDGDRYTPVIRRGMRKPDSPKSGKG"
     gene            complement(1086..>1969)
                     /locus_tag="PK264_13025"
     CDS             complement(1086..>1969)
                     /locus_tag="PK264_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HRD76836.1"
                     /translation="TNVGGSETKVTRYYYDRVWAEGRINSSSFKNQSGHENPEPRLKA
                     LTVTASDAALGAFKPGEDVISRVSAGAKLPVPTAAVEAAKSRFGAAHLTNDAVCICAD
                     PGAPRIGDLRVSYRILPAGPLSIIGAQSGSDFAPYQTKAGDKLLMVETGLKPADEMFK
                     GAQRSNTILTWVIRAVAVLVMWIGFSMLFGPFVVLADVIPFLGSLVGIGAGLAAAVAT
                     LVVAPVSIAIAWFAYRPIVSAIVLGVGLVLAFLLKRASPKRTPQPVPGTLQPAGMPAA
                     GVASTPPKSFLPPRAKR"
     assembly_gap    1970..1979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1980..2400)
                     /locus_tag="PK264_13030"
     CDS             complement(<1980..2400)
                     /locus_tag="PK264_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76837.1"
                     /translation="MADNDDGSFQASSDAGGDVYTETEHTSWFERIKNALVGILVGLA
                     LVPASIVGIFWNEGRAVTTSRSLTEGAGLVVNVDAAKVDPANQGKLVHVAGPVKAASM
                     LKDVDLGVSADALRLVRVIEMYQWKERRETETVKHYGG"
     gene            complement(2527..3222)
                     /gene="hisG"
                     /locus_tag="PK264_13035"
     CDS             complement(2527..3222)
                     /gene="hisG"
                     /locus_tag="PK264_13035"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRD76838.1"
                     /translation="MTERLVLALPSKGRLMEETEKLFADAGLPVRRVGHARGYRGEIE
                     GLPNVEVAFVSASEIAERIKSGKAHLGVTGEDLVAELVPAHRAAVTFLKALSFGHANV
                     IVGIPAAWIDVVSMASLERAAVAFRRQHGRHMRVATKYGRLTRSYFADMGVSTYRIVP
                     SLGATEGAPAMGTAELIVDITTTGKTLEANGLKVLETILKSEANLVASKAATWPPEVR
                     EAENILMKRLGIM"
     gene            complement(3215..4402)
                     /locus_tag="PK264_13040"
     CDS             complement(3215..4402)
                     /locus_tag="PK264_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRD76839.1"
                     /translation="MAQRIAEKLGRLDRQSATILNIFAGAGYEQVAPPIIQPASLFLD
                     VVGEQLRNRTYVFNDPDGEELCLRPDLTVPTCRLHLERHGLKGDVSAKYCYSGPVFRY
                     QPGGGDATHLREFRQEGLEAFSPLDRETTEVEVLTKTLEALKASGLKGSQLRVGDISI
                     FLALLKAIDMPPRWRDRLRQSFWRPEAFRAELKRLTSMPKAALTGLPEDLIARLDPND
                     PKAAETEVASYLADKALEPIGERSIAEISDSLLTAAVDARTPALPASVADLIERYLAI
                     AAPVRAATARLKDLAREKGLDIGTALDAFIRRNELLTAAGVDLGKAEFSAEFGRNFEY
                     YSGFVFEVVVPSLGPKSPVAGGGRYDGLMKAVGAAGSIAAVGAAVYSDRLALAIEEAS
                     KGD"
     gene            complement(4410..5903)
                     /gene="hisS"
                     /locus_tag="PK264_13045"
     CDS             complement(4410..5903)
                     /gene="hisS"
                     /locus_tag="PK264_13045"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRD76840.1"
                     /translation="MMTKHNQPKARLPKGFQDTFAEDLRTTARLVETIREVYELYGFE
                     PLDTSIVEYTDALGKFLPDQDRPNAGVFSFQDEDEQWMSLRYDLTAPLARHVAENFDK
                     LPKPFRRYAVGPVFRNEKPGPGRFRQFMQFDADTVGSESIAADAEICMLAADAMERVG
                     VPRGKYRIKVSNRKVLNGLMEAAGLAGEAMAGQRLTVLRAIDKLDKFGPDGVRLLLGP
                     GRRDESGDFTKGAGLEASAIDTILAFTAAGSGATSSNEAVLDGFRRAVGSTDVGAEGV
                     AELAAIAAITKAAGYDETRVVIDSSIVRGLEYYTGPVFEAELTFEVKNEDGQTVRFGS
                     VGGGGRYDDLVARFTGQRVPATGFSIGVSRIEAAFRAISKSDSKAGSGPVVVLVMDKG
                     ELPRFQRHVQALRGAGIRAELYLGASGMKAQMKYADRRNAPCVVIQGGDEIARGEVQV
                     KDLVEGARAAASIKDSKAWREGRPAQMSVPEARLVEAVREILARHAG"
     gene            6185..>6556
                     /locus_tag="PK264_13050"
     CDS             6185..>6556
                     /locus_tag="PK264_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HRD76841.1"
                     /translation="MLKATQAELTHRVIGFIDRNTTELAAAVHRVPVGTYADPGHLKR
                     EQQRLFRETPQLFCLSGELHKPGDWKAEDLSGVPILVVRGQDRSINAFLNVCRHRGAR
                     LVEGSGSGKRLFVCPYHAWSYK"
     assembly_gap    6557..6581
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6582..7336
                     /locus_tag="PK264_13055"
     CDS             <6582..7336
                     /locus_tag="PK264_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HRD76842.1"
                     /translation="DEGFEGLECKDHGLTRLPAAEKHGLVWVTGTPGPEIDVDALLGG
                     LAPEMAAYGIASHIPFKTARLEKQMNWKLAIDTFLETWHVATLHVATVAPIFQPNVNA
                     FDAFGRNGRLIIPRRTIVEMRGGKGPENGFLKHTAIVYQMFPNAALVWQADHFELWRA
                     FPAGDDPARCITEATLYTPEPATSDKAKAYWEKNFDLLMRTVEAEDFPVCEGIQRGLL
                     SGAQEHITFGRNEPALGHYHASLAAALGAQHG"
     gene            7329..7631
                     /locus_tag="PK264_13060"
     CDS             7329..7631
                     /locus_tag="PK264_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76843.1"
                     /translation="MAEPARKRLTFVLIARIPLEGIGAFQAYESAVLPVLAEHGARLE
                     RRLRSETGTIELHIVSFASEAAFEAYRADPRRTALAPDLARSNAQLELLPLEDVTA"
     gene            7777..8745
                     /locus_tag="PK264_13065"
     CDS             7777..8745
                     /locus_tag="PK264_13065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD76844.1"
                     /translation="MWSAEHHFDGYTMCPNVMQFLTYMAGRTKHARLGSMVTVLPWHE
                     PVRIAEEVSVLDHLSGGRVILGIGRGLGRTEFDGFRIPMGESRERFVAYSEAILGALE
                     TGVIKSDVPAYRQPPTSIRPTPWKSFKGRVYAAAVSPESAKIMARLGVGLLVIAQKPW
                     DKTIAELEAYRALYREMNGTEAPRPIVVSWVACHEDASEAQRMYERYIRRYSRSATDH
                     YEFYNPGLADIKGYEYYGALAKNIEKQGLDKFVEFLASLQIWATPDAVHDRIAEYCRL
                     LDAESFVGVFGYGGMPADIAERNLRLFARTVLPRLKARRVGADRAA"
     gene            8847..9434
                     /locus_tag="PK264_13070"
     CDS             8847..9434
                     /locus_tag="PK264_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76845.1"
                     /translation="MTARFPSLLKTVAFAAIAVSAVVAPAAAFDPKSTHVVIVIDDSR
                     ADSMSARNRAAKRLLNAAASVFADKGFTVSIEPVFDAGTRIAQPSKSVRDYVTGLKAA
                     KTVVVTLDLGLTVNKSAAPNATTMTTRYALNMNILDGATAKFLGKDDSSGALADIAGA
                     CAKDAHCLLEETGSGMLPIVSARASAMADRLIAMK"
     gene            complement(10107..10487)
                     /locus_tag="PK264_13075"
     CDS             complement(10107..10487)
                     /locus_tag="PK264_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HRD76846.1"
                     /translation="MSARAELSASLFGRVREHHRFLVRQHLSLIEQLEAMIVAFDARI
                     EAALAPFRDSIERLKEIPGLSDTSAQILVAEIGLDMSLFPRAGHLLSWAGLVPRLDES
                     AGRRRSTRIRKGAPRLKPVLVQCG"
     gene            complement(10730..>12264)
                     /locus_tag="PK264_13080"
     CDS             complement(10730..>12264)
                     /locus_tag="PK264_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRD76847.1"
                     /translation="KLSLVAWERSVAAAEPAARTFIRTDIVDRAGRLIATDVPISSLW
                     ADPAVILDRDEVLDRLSEIFPDLDTAETRRLIADRSKRFVWLRRHLRPIDAQRVHELG
                     LPGLALKREPKRAYPLGRLAGHIVGTVNIDNKGTAGAERAIDEAGLAEPAYGPGRPRA
                     ESVRLAIDMGAQHLLSAGLEVERQRYKAKGAGAIVMDIVTGEILAAVSLPGIDPALPV
                     ETLDPERLDKLAGGVFELGSINKIFAVAMAFEQGFATPQRIYDVRQPIVIGRHTIRDL
                     HPQYRPLTVREIFITSSNIGAGMLALEAGAEHQRAFLDGLGLISPLRTEIGAIAPPLL
                     PTRWDKIETVTIAYGHGLAVAPVQLLASAATLFNGGYRVTPHFHAAKPGTATERVRIL
                     SAETSARMRELMRQNVTIANGTGRRAETAGVDIAALELGGKTGTAEQPGAGGYREKAV
                     ISSFLAITPASDPRHIALVMLFEPAGTEETKGQITAGVTAAPATARILARLAPALGLG
                     RW"
BASE COUNT         2031 a         4015 c         4216 g         1967 t
ORIGIN      
        1 aaaggcagag aagggtggca aggggcagaa aggagacgac acagagtgac gtcgcgcgtc
       61 cagaatagtt cgcccggagc tgcctgactt cttgcagctc cacgtgatcc cagcagtgac
      121 agggtgcgcc atgaccgcga ctagctcggc cataggtttc gtcggcctcg gcgtgatggg
      181 cgaggcaatg tgccggaacc tcgcgacgaa atcgaaagcg cgggtcatcg cctacgatcg
      241 ccgcgccgag ccgctcgagc gtctcgcggc cgtgggcgtc acccgggcgg gctcgctcgc
      301 agcacttgcg gccgacgccg atacgatcct gatgtgtctc cccggcgggc ccgaagtcga
      361 ggcggtggcc cttggcaagg acggcctcgt cgcgcatgca cggcgcggtc agacgctcgt
      421 cgacatgtcg acggccccgc cgcgcctgat gcgcgagatc gagcggcagc tcgtcccgcg
      481 cggcgcgcgc tttgccgatg cgcctgtcgc gcgaacccgg caggcggccg tcgacggaac
      541 gctctcgatc atggtcggtg gtgcagccga tctcttcgcc gagctgaagc cgatcctcgc
      601 cacgatgggg agcgaggtca cgcactgcgg cgcggtcggc gcaggccaga tcgtcaagct
      661 catgaacaac atggtcgtct tcgaaacgac gatggcactc gccgaggcga tctcgatcgc
      721 ggaggcctcg ggcgtcgacg gcaagctcct gttcgagacc atggcgaagg gctcggccga
      781 cagcttcgtc ctgcgcaatc acggcatgaa gaatctggtg ccgcagagct atccggagca
      841 ggccttctcg gtgcgctacg cgcagaagga cctttcctat gcgcgtgacc tcgcgcgcga
      901 ggcgggtgtc gatgcgcgtg cggccgatct cgtcgccagg ctcttcgacg aggcgatcgg
      961 acgcggcgac ggcgaccgct acacgcccgt catccgccgc ggcatgagga agcccgacag
     1021 ccccaagtct gggaagggct gatcacgacg cctgctcgcg cgtcgcgatc agggcacatc
     1081 gccggtcagc gcttggcgcg cggtggcagg aagctcttcg gcggcgtcga cgcgacaccg
     1141 gcagccggca tgccggcggg ctgcagtgtg ccgggcacag gttgcggggt gcgcttcggc
     1201 gacgctcgct tcagcaggaa ggcgagcacc agcccgacgc caagcacgat cgcggagacg
     1261 atcggacgat aggcgaacca ggcgatggcg atcgacacgg gtgcgacaac cagcgtcgca
     1321 acggctgccg cgaggccggc gccgatgccg acgaggctgc cgaggaacgg gatcacatcc
     1381 gccaggacga cgaatggccc gaacagcatc gagaatccga tccacatcac gaggacggcg
     1441 accgcccgga tgacccaggt caggatcgtg ttggagcgct gcgcaccctt gaacatctcg
     1501 tccgccggtt tgaggccggt ctcgaccatc aggagcttgt cgccggcttt cgtctggtag
     1561 ggtgcgaaat cgctccccga ctgagcgccg atgatgctga gcgggcctgc gggcaggatg
     1621 cggtaggaga cgcgcaggtc gccgatgcgc ggggcgccgg gatcggcgca gatgcagacg
     1681 gcatcgttcg tcaggtgggc agccccgaag cgggacttgg ccgcctcgac cgcggctgtc
     1741 ggaacgggaa gcttcgctcc ggccgaaacg cgcgagatca cgtcttcccc tggcttgaag
     1801 gcgccgagcg cggcgtcgct cgccgtcacg gtgagcgcct tgagccgcgg ctcggggttc
     1861 tcgtggccgg actggttctt gaacgagctc gagttgatgc ggccctcggc ccagacgcga
     1921 tcatagtagt agcgggtgac cttcgtttcc gagccgccga cgttggtctn nnnnnnnnna
     1981 gccgccgtag tgcttcacgg tctcggtctc gcgccgctcc ttccactggt acatctcgat
     2041 cacgcgcacc aggcgcagcg cgtccgccga tacgccgagg tcgacgtctt tcagcatgga
     2101 tgccgctttg acggggccag cgacatgtac gagcttgccc tgattggcag gatcgacctt
     2161 ggcagcgtcc acgttgacga cgaggcccgc gccctcggtc agcgagcgtg acgtcgtcac
     2221 ggcgcgtccc tcgttccaga agatgccgac gatcgaggcg ggaacgagcg cgaggccgac
     2281 cagaatgccg acgagcgcgt tcttgattcg ctcgaaccag gaggtgtgct cggtctcggt
     2341 atagacgtcg ccacctgcat cgctcgacgc ttggaacgag ccatcgtcgt tgtcggccat
     2401 cggtgtctcc tttgcattga acttggctcg gcggaatgcg gggcgccctt gcccgatatc
     2461 gcgggaggcg agcgcctcaa cctaatccaa gctgtgagga aaagcacctt cctcgaaacg
     2521 gaagggttac atgatgccga gccgcttcat gaggatgttc tcggcctcac gcacctcggg
     2581 cggccaggtg gcggccttgg acgccacgag attggcctcg gatttcagaa tggtctcgag
     2641 caccttgagg ccgttggcct cgagcgtctt ccccgtcgtg gtgatatcga cgatcaactc
     2701 ggccgtgccc atggccgggg ccccctcggt tgcaccgagg ctcggcacga tgcgataggt
     2761 cgacacgccc atgtcggcga agtagctgcg tgtcaggcgg ccgtacttgg tcgcaacgcg
     2821 catatggcgg ccatgctggc gccggaatgc gaccgcggca cgctcgagac tcgccatcga
     2881 caccacgtcg atccaggccg ccgggatgcc gacgatgaca ttcgcgtggc cgaaactgag
     2941 tgccttcagg aatgtgacgg ccgcacgatg cgccggcacg agctcggcca cgagatcctc
     3001 gcccgtcacg ccgagatgcg ccttcccgga cttgatgcgc tcggcgatct cggaggccga
     3061 cacaaatgca acttcgacgt tgggaaggcc ttcgatctcg ccgcgatagc cgcgggcatg
     3121 cccgacccgg cgcaccggca gtccggcatc ggcgaagagc ttctccgtct cctccatgag
     3181 gcggcccttc gagggcagcg cgagtaccag ccgctcagtc acctttggag gcctcctcga
     3241 tggcgagcgc cagccggtcc gaatagaccg cggcgccgac cgcggcgatg ctgcccgcgg
     3301 caccgacggc cttcatcagc ccatcgtagc gtccgccgcc ggcgacgggg ctcttaggac
     3361 cgagcgaggg caccacgacc tcgaacacga agcccgagta gtactcgaag ttgcgcccga
     3421 actcggccga gaactcggcc ttgccgagat cgactccggc tgccgtcagc agctcgttgc
     3481 gacgtatgaa ggcatcaagg gccgtaccga tgtcgagacc cttctcgcgc gcgagatcct
     3541 tgaggcgcgc tgttgccgca cgaaccggcg ccgcgatcgc gaggtagcgc tcgatgagat
     3601 cggcgacgct cgccggcagc gcgggcgtgc gtgcgtccac ggccgccgtc agcaggctat
     3661 cggaaatctc ggcaatcgag cgctcgccga tgggctcgag cgccttgtcg gcgagatagc
     3721 tcgcaacttc ggtctcggcc gccttggggt cgttcgggtc gagccgcgcg atgagatcct
     3781 cggggaggcc ggtgagggcg gccttcggca tcgaggtcag ccgcttcaac tcggcgcgga
     3841 acgcctccgg gcgccagaag ctctgccgca gccggtcgcg ccagcgcggc ggcatgtcga
     3901 tcgccttcaa gagggcgagg aagatgctga tatcgccgac gcgcagctga ctgcccttca
     3961 ggcccgaggc cttgagcgcc tcgagcgtct tcgtgagcac ctcgacctcg gtcgtctccc
     4021 ggtcgagcgg ggagaaggcc tcgagaccct cctggcggaa ctcgcgcagg tgggtagcgt
     4081 cgccgccgcc cggctggtag cggaacacgg ggccgctgta gcaatacttg gcgctgacat
     4141 cgcccttgag gccgtgccgc tccaggtgca gccggcaggt cggtacggtg agatcggggc
     4201 ggaggcagag ctcctcgccg tccggatcat tgaagacgta tgtgcggttg cgcagctgct
     4261 cgccgacgac atcgaggaac agcgaggcag gctgaattat cggcggggcg acctgctcgt
     4321 agccggcgcc ggcaaagatg ttgaggatcg tcgccgactg ccggtcgagg cggccgagct
     4381 tctcggcaat gcgctgagcc atggccggat cagcccgcgt gccgcgcgag gatttcgcgc
     4441 acggcttcga cgagacgcgc ctcgggcacg gacatttggg ccggccggcc ctcgcgccac
     4501 gccttgctgt ccttgatcga cgccgctgcg cgggcgccct cgaccagatc cttcacctgc
     4561 acctcgccgc gtgcgatctc gtcgccgccc tgaatgacga cgcacggtgc gttgcgccgg
     4621 tcggcatact tcatctgcgc cttcatgcct gaggcgccga ggtagagctc ggcgcgtatg
     4681 ccggcgccgc gcagcgcctg cacgtggcgc tggaagcgcg gcagctcgcc cttgtccatg
     4741 acgagcacga cgacggggcc gctgccggcc ttgctgtcgg acttcgaaat tgcgcggaaa
     4801 gctgcctcga tgcgcgagac gccgatcgag aatccggtcg cgggcacacg ctggcccgtg
     4861 aagcgcgcga cgagatcgtc gtagcgcccg ccgccgccga ccgagccgaa gcggacggtc
     4921 tggccgtcct cgttcttcac ctcgaaggtg agctcggctt cgaacacggg gcccgtatag
     4981 tattcgaggc cgcggacaat tgagctgtcg atgacgacgc gcgtttcatc gtagcccgcg
     5041 gcctttgtga tcgccgcaat cgcggcgagc tcggcgacgc cctcggcgcc aacatcggtc
     5101 gagccaacgg cgcggcggaa tccgtcgagg accgcctcgt tcgaagacgt cgcgccgctg
     5161 ccggcggccg tgaaggctag aatggtgtcg atggcactgg cctcgagccc cgcacccttc
     5221 gtgaagtcgc cgctttcgtc gcgccgtcct gggccgagca ggaggcgcac gccgtcgggg
     5281 ccgaacttgt cgagcttgtc gatagcgcga agcacggtaa gcctctgccc tgccatggcc
     5341 tcgcccgcga ggccggcggc ctccatgagg ccgttcagga ccttgcgatt tgagaccttg
     5401 atgcggtact tgccgcgcgg cacgccgacc cgctccatgg catcggcggc gagcatgcag
     5461 atctcggcat ccgccgcgat actctcagag ccgaccgtat cggcatcgaa ctgcatgaac
     5521 tggcggaagc ggccggggcc gggcttctcg ttgcggaaca cggggccgac cgcatagcgg
     5581 cgaaacggct tcggcagctt gtcgaagttc tcggcgacgt gccgggcgag cggggcggtc
     5641 aggtcgtagc ggagactcat ccactgctcg tcctcgtcct ggaacgagaa gacgcccgcg
     5701 ttcggccggt cctggtcggg caggaacttg ccgagcgcgt ccgtgtactc gacgatcgag
     5761 gtgtcgaggg gctcgaagcc gtaaagctcg tagacctcgc ggatggtctc cacgaggcgg
     5821 gcggtcgtgc gcaggtcctc ggcgaaggta tcctgaaatc ctttgggcag ccgcgccttg
     5881 ggctggttgt gctttgtcat caggaaccgg gttcgtatgg ccagattggc ggccagattg
     5941 gcggactgtc ggcgcccgct ccgccgcatt cccgcgagga acggtgcggg ccgcggcgag
     6001 gcattggact acgggcgggc ttatagccga agcgggcgag gttaggaagc tgcgaccaaa
     6061 gggcatggcg aacggctcgc gttgccattt acctcgcgcg acctgcccgg tagtgttcac
     6121 atatatcagg cctagacggt cgcgcggcgg ccggcgcggg tcgcacaacg ttccagggcg
     6181 gccgatgctg aaagcgaccc aagccgagct cacccaccgc gtcatcggct tcatcgacag
     6241 gaacacgacc gagctcgccg cagcggtgca tcgcgtgccg gtcgggacct atgccgatcc
     6301 cggccacttg aagcgcgagc agcagaggct ctttcgcgag acgccgcaac tcttctgcct
     6361 gtcgggggag ctgcacaaac ccggcgactg gaaggcggaa gacctatcgg gtgtgccgat
     6421 cctcgtcgtc aggggccagg accgcagcat caacgccttc ctgaacgtgt gccggcatcg
     6481 cggcgccagg ctcgtcgaag gcagtggctc ggggaagcgg ctgttcgtgt gcccgtatca
     6541 cgcctggagc tacaagnnnn nnnnnnnnnn nnnnnnnnnn ncggacgagg gcttcgaggg
     6601 cctcgagtgc aaggaccatg gcctgacgcg gctgccagcg gcggaaaagc acggccttgt
     6661 gtgggtcacg gggacacctg ggccggaaat cgacgtcgat gctcttctcg gcggcctcgc
     6721 cccggagatg gccgcctacg gcatcgcgag ccatatcccg ttcaagacgg cgcggctcga
     6781 gaagcaaatg aactggaagc tcgcgatcga tacctttctc gagacgtggc acgtcgcgac
     6841 gctgcacgtc gcgaccgtcg caccgatctt ccagccgaac gtcaacgcct tcgacgcttt
     6901 cgggcgcaac ggccggctca tcatcccgcg tcggacgatc gtcgagatgc gcggcggcaa
     6961 gggtcccgag aacggcttcc tgaagcacac cgccatcgtc taccagatgt tcccgaacgc
     7021 ggccctcgtc tggcaggccg atcacttcga actctggcgc gcatttccgg ccggcgacga
     7081 tccggcgcgc tgcatcaccg aggcgacgct ctatacgccg gagccggcaa ccagcgacaa
     7141 ggcgaaggcc tactgggaaa agaatttcga cctcctgatg cgcaccgtcg aagcggagga
     7201 cttccccgtc tgcgaaggca tccagcgagg cctcctctcg ggcgcgcagg agcacatcac
     7261 cttcgggcgc aacgagccgg cactcgggca ctatcacgcg agccttgccg cggcactggg
     7321 ggctcagcat ggctgaacct gctcgcaagc ggctcacctt tgtgctgatc gcacgcattc
     7381 ctctcgaggg cattggcgca ttccaggcct acgagagcgc cgtgctgccg gtcctcgcgg
     7441 agcatggcgc tcgcctcgaa cgccggttgc gttcggagac tggaacgatc gagctgcata
     7501 tcgtcagttt tgcctcggag gcggccttcg aggcctaccg ggccgacccg cgacggacag
     7561 cactcgcgcc agatctagcg cggtcgaatg cccagctcga gcttttgccc ctcgaggacg
     7621 tgaccgcata agcccgccga ccgcgccgga ggaaccccca tggacgtcgg aatgagcgcc
     7681 ttcttccaga atttcgacga gcccgcgagg gaccgcgaca tctggcgcga gcaggtggcg
     7741 ctcgccgacc ttgccgagcc gctcggcttc taatcgatct ggtcggccga gcatcatttc
     7801 gacggctaca ccatgtgtcc gaacgtgatg cagttcctca cctatatggc gggacggacg
     7861 aagcacgcgc ggctcggctc gatggtgacg gtgctgccct ggcatgagcc ggtgcgcatc
     7921 gccgaggaag tctcggtcct cgatcacctc tcgggtggcc gtgttatcct cggcatcggc
     7981 cgcggtctcg ggcgcacgga attcgacggc ttccgcattc cgatggggga atcgcgcgag
     8041 cgcttcgtcg cctactccga ggcgatcctc ggcgcgctcg agacgggcgt catcaagagc
     8101 gacgtgccgg cctatcgcca gccgccgacc tctatccggc cgacgccctg gaaaagcttc
     8161 aagggccgcg tctatgcggc agcggtctcg cccgagtcgg cgaagatcat ggcgcggctc
     8221 ggcgtcggac tcctcgtcat cgcgcagaag ccctgggata agacgatcgc ggaactcgag
     8281 gcttatcgtg cgctgtatcg cgagatgaac ggcacggagg cgccacggcc gatcgtcgtc
     8341 tcctgggtcg cctgccacga ggacgcgagc gaggcgcagc gcatgtacga gcgctacatc
     8401 cgccgctaca gccgctcggc gaccgaccac tacgagttct acaatccggg cctcgccgac
     8461 atcaaaggct acgagtacta cggtgccctc gcgaagaaca tcgagaagca gggcctcgac
     8521 aagttcgtcg agttcctggc gagcctgcag atctgggcca caccggacgc cgtgcacgac
     8581 agaatagccg aatactgccg cctcctcgat gccgagagtt tcgttggcgt gttcggctac
     8641 ggcggcatgc cggccgacat cgccgagcgg aacctgcggc tcttcgcaag aacggtgctg
     8701 ccgcggctca aggcgcgcag ggtcggcgcg gacagggcgg cgtgaggacc gccgccattg
     8761 gccggatccg acagccggtc gcttgatcaa aagcggctct ggctggcgca ccagctgatg
     8821 gtcccctcac caccaggagc tttcctatga cggcgagatt tcccagcctg ctgaagaccg
     8881 ttgcctttgc agccatcgca gtgagcgcgg tcgtggcacc ggccgcggca ttcgatccca
     8941 agagcacgca tgtggtgatt gtcatcgacg actcgagggc cgactcgatg tcggctcgca
     9001 atcgtgccgc aaagcgactt ctgaacgccg cggcctctgt gtttgccgat aaggggttca
     9061 cggtgagcat cgagcccgtt ttcgatgccg gaacccggat cgcgcagccg agcaagtccg
     9121 tgcgggacta tgtgaccggc ctcaaggccg ccaagaccgt cgtcgtgacg ctcgacctcg
     9181 ggctcaccgt caacaagagc gctgcgccca atgccacgac catgacaacg aggtacgcgc
     9241 tgaatatgaa catcctcgac ggagcaaccg cgaagttcct cggcaaggat gacagcagcg
     9301 gtgcactcgc cgacattgcc ggcgcttgcg ccaaagacgc gcactgcctc ctcgaggaga
     9361 caggaagcgg catgcttccc atcgtttcgg cccgagccag cgccatggcc gatcgactga
     9421 tcgccatgaa gtgatcgctg cgacggcgga aatgcgccgc gcgtcccacg ctcgccatcc
     9481 tcgcgaaggc gaggacccag tgcaccgtgc gtgggaacgg tgcgagctgc ctcgagggga
     9541 attaggtgcc gagcttcgcc gccgtgacgg tggggtgagg aggcggcatg actgaggagg
     9601 gcggggcggg aagagttcgg gtgctgcgcc gcccgtcgcg ctggatcccg gagcgctttc
     9661 gcggcgaaag ggccgctccg gcgcccggga tgaacgtggt gaggcgctat catcgcgcgc
     9721 cgcggctgtg gctatctttg acgacccaat tgtcgatcga cgacggtaac gcgtgctggg
     9781 catgatcggg accgggggcg atgtcggagg ggctgtcctc tacagaagct gatcttgctc
     9841 tgatgcggtc atgcggcggc gcggatgtcg acggcaaagc cgagagaacg gatgcgcttg
     9901 gcgagcttcc cggcttcccg agccgggttg cgacgggcga agtggtcggc gccgagatcc
     9961 tggtagaggg tgccgtcctt gaggatatga taggcggttt ccaggatcga cgcggcgacg
    10021 gcgacagccg ccttcttctg gccgcagcgc gccttgtggc gcaggaactg cgcctggaag
    10081 tagctgctct tcttgcgcgc cgcggctcag ccacactgga cgagcaccgg cttcagccgg
    10141 ggtgccccct tcctgatgcg cgtcgagcgg cgccggcccg cactctcgtc gaggcgtggc
    10201 acgaggcccg cccacgacag gagatgcccc gccctcggaa acaggctcat gtcgaggccg
    10261 atctcggcaa caaggatctg cgccgacgtg tcggacagcc ctgggatctc cttcaagcgc
    10321 tcgatgctgt cacgaaaggg tgcgagcgcg gcctcgatcc gggcgtcgaa ggccacgatc
    10381 atcgcctcca gctgctcgat gagcgagagg tgctggcgga caaggaagcg gtgatgctcg
    10441 cgaacgcggc caaagagcga ggctgagagc tctgcccgcg ccgacacgcg tcacagcgcg
    10501 ccggccggcc aaaactcgga acgggttgca tgggaccgcc ggagtttggg ccgtgcctga
    10561 cacgggccgg ccctgacggg ggcaattgat gcggttctct cgccgggcgc cggtgctccc
    10621 tcctcacgtc aggatcgcca cggcgctcca atagggatcg atgaccgcgg ctgaactttt
    10681 cccgagcaga ggacctcggc gcgagcatca cgccgcgcgc tcagtgccat caccaccggc
    10741 cgagtccgag cgccggcgct aggcgggcga ggatgcgggc ggtcgccggc gccgcggtga
    10801 cgcccgcggt gatctggccc ttggtctcct cggtgccggc cggctcgaac agcatcacga
    10861 gggcgatgtg gcgcggatcg cttgccggcg tgatcgcgag gaaggaggag atcacggcct
    10921 tctcgcgata gccgccggcg cctggctgct cggccgttcc cgtcttgccg ccgagctcga
    10981 gcgccgcgat gtcgacgcct gccgtctccg cgcgacggcc cgtgccgttg gcaatcgtaa
    11041 cgttctggcg catcagctcg cgcatgcggg cgctggtctc ggcggagagg atgcgcacgc
    11101 gctccgttgc ggttccgggc ttggccgcat ggaagtgcgg cgtcacacga tagccgccat
    11161 tgaagagcgt cgcggccgaa gcgagcagct ggaccggcgc caccgcgagc ccgtgaccgt
    11221 aggcgatcgt cacggtctct atcttgtccc agcgcgtggg gagcagcggc ggcgcaatgg
    11281 cgccgatctc ggtgcgcagc ggcgagatga ggccgagccc gtcgaggaag gcgcgctgat
    11341 gctcggcgcc ggcctcgagc gccaacattc cagcgccgat attggacgac gtaatgaaga
    11401 tttcgcgcac ggtgagcggg cggtactgcg gatggagatc gcggatggtg tggcggccga
    11461 tcacaatcgg ctggcgcacg tcgtagatgc gctgaggcgt cgcgaagccc tgctcgaagg
    11521 ccatcgccac cgcgaagatc ttgttgatcg agccgagctc gaacaccccg cccgcgagct
    11581 tgtcgagccg ctcgggatcg agtgtctcga ccggcagcgc gggatcgatg cccggcagcg
    11641 acaccgccgc cagtatctcg cccgtcacga tatccatgac gatcgcgccg gcacccttgg
    11701 ccttgtagcg ctgccgctcc acctcgaggc cggcggaaag caggtgctgc gcgcccatat
    11761 cgattgcgag cctgacagac tccgcccgcg gccgaccagg tccgtaggca ggctcggcga
    11821 gtccggcctc gtcgatcgcg cgctcggcgc cggcggtccc tttgttgtcg atgttgaccg
    11881 taccgacgat atgcccggcg aggcggccga gcggataggc gcgcttcggc tcgcgcttca
    11941 gggcgaggcc cggcaggccg agctcgtgga cgcgctgcgc atcgatcggg cggaggtgcc
    12001 ggcggagcca gacgaagcgc ttcgaacgat ccgcgatgag ccggcgcgtc tcggcggtgt
    12061 cgaggtcggg gaagatctcg ctgagccggt cgaggacctc gtctcggtcg aggatcacgg
    12121 ccggatcggc ccagagcgag gagatcggca cgtcggttgc gatgagccgc ccggcacgat
    12181 cgacgatgtc ggtgcggatg aacgtgcgcg cggccggctc ggcggcggcg acggaacgct
    12241 cccaggcgac cagcgagagc ttgg
//
LOCUS       DAORNH010000121        12240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3908_length_12240_cov_2.547230, whole genome shotgun sequence.
ACCESSION   DAORNH010000121 DAORNH010000000
VERSION     DAORNH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3908_length_12240_cov_2.547230"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..3049
                     /locus_tag="PK264_13085"
     CDS             416..3049
                     /locus_tag="PK264_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76848.1"
                     /translation="MASTSGDTARGRSADGWDAIVKDERPKRDWKKLLLVVGLGVLSW
                     VATYVGMLELIEANMGELPLVHKGIIGFSVAMLMVMVVWLLDQIFAPGAGFVAKLSYI
                     FGYVFLTIISVGFGFGFYWKVLESRSEGTRSAESAIGQVQNSLHAATTRLEQLVATLE
                     SLTLLSTQKAEFERANGTSCPASRPGDGPRRKMREDDAQRFTFASDFVKGRVANVKAE
                     MKALDVDLAKIVRDDKSAIDAKSGTRNEFMRALGRKLELTVTGFNAFRGDPQLRQIRQ
                     DLAERGEQSSFPDGKGKTFACPDGQLQVALRGVVRAIDQLPELERPKIAVVEGSEAVI
                     EAFRRLTATFYGALAFKLPPSPEELRELQKKAIAAAESGATGAAARIPSLEQAGLSKR
                     DYIPLAIAVFVDLCLLLVSMGRPSNRLNSLVPKMRAAERGPVYQILSRFNQIHHDGEM
                     RQHFELFRHVVFDYLNDYYVAVPLDAPKVVFDWLPVPKDKVRTTMEHRQRMLSTAERA
                     ELQREAHLLANLFASFEQEKIFFRIDDRWLGRMTLQVPTSEQARKKLRRQGSKFAEAE
                     AFRIYKFSDGAWSEIILGAVMGAARRVEARRVASGEPEPVLPMPSARTQPMPVAEPGD
                     VLAHDPLSAVRPFAMRPPVRPASEKIEPTLASVAATGTRRQPRTVVPDERHDAQFGPY
                     ARYAEPAFEDAYVGESANNNTAPNPKPVVGVTADGARAVQPSPLPPADILPASVPTAA
                     SVSATTAGAAVGSLLRRLADTPRATRTGAVEPDTMTVELVERTATVHVPKEGTLSDVV
                     QRALKTAKAAHVETVAPTVIAHQPDVAPGPSETVHDQMLAVIKAAEAAPVFEVEAPHA
                     GDTSDPEMARLSARFAARVAP"
     gene            complement(3278..4513)
                     /locus_tag="PK264_13090"
     CDS             complement(3278..4513)
                     /locus_tag="PK264_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD76849.1"
                     /translation="MKIQEITSPGGIKGWLVEERKVPLLAFRFVFEGGGSEDPIGKEG
                     VAELLATLLDEGAGDLDAQKYQERMEELAMRMSFEATDDAFYGTFQSLTANRDASVEM
                     LRLALAKPRLDAEAIERMRKQKLANLAYALKNPSTIAGREWAMLAYPNHLYSRLVTEK
                     SVAAITRDDIETVRRKVFAREKLVAVGDISAKDFGLLLDKVFGELAVKANITPIPPIT
                     IPSGAKEKIVEFDVPQSVVMFGMAGIPRNDPDFIPAFVMNEILGGGGFHTRLMEEVRE
                     KRGLAYGVSSYLQTLRASNSFGGRVATKNESVGQSIDIIRAELKRMAEGPTEKELADI
                     KSYLIGSYPLRFDTNAKIANVLLGVLYDDLGIDYVDKRNKLIEAVTVADIKRVAARLL
                     KPENMITVIVGKPKLPGRG"
     gene            complement(4640..6058)
                     /locus_tag="PK264_13095"
     CDS             complement(4640..6058)
                     /locus_tag="PK264_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD76850.1"
                     /translation="MRRLHPRSNLSRTMGLVFLMTLLATLTGPGSAWSQVKATEMRLA
                     NGMQIIVIPDNRAPVVTHMVWYRVGRADEPAGMSGVAHFLEHLMFKSTEKIAVGEFSK
                     IVSRLGGNDNAFTGQDVTAYFQRVHKDRLEKVMGMEADRMVNLRLTEKEVLTERDVIL
                     EERRTTVESKPGSILGEQMDAALYLVHPYGQPVIGWENEIARLTPQNAIDFYKRFYAP
                     NNAILVVSGDVTPEHVKALAEKTYGLVPANPAVMARPRAPEPLHRVARRVELRDPRAG
                     RYSLHRYYLAPSYASAEAGEAEALELLMKIMASGGTSRLYKKLVADERIAANAGGGYG
                     GYGRDSGKISFTVVAADGVPLSRAEASLDELIADIRAKGITEAELARAKTLYMADYVY
                     LSDNQYSLAQMYGWAITVGRTLKDVEEWPARINRVTLDDIKKVAAKYLDIRRSVTGTM
                     LPEPASPDARRVDPAVPPKNRS"
     gene            6363..7589
                     /locus_tag="PK264_13100"
     CDS             6363..7589
                     /locus_tag="PK264_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76851.1"
                     /translation="MDDPLKVTPAQPDRPRARRWGARLLIRLFLMVLAIEGLSYALLR
                     FGPQALIQTVYHPPVIGRAAFETFMAERHPILSWPTRGRDGRDREIPLDERGARTSMT
                     NVVFSNGDTCLSAYGDGAVRADGVADHHAWSDLLARRLACRVDNFGISGYGADQAVLR
                     LLLNPADAAKAMVLGVSPAALLRMGSQWPFLLDGPSAGLAILTDMTGRLDLVPAHDLT
                     YEGFLGLARDPALYLGHERYLPGSPYGPEPFAFPYSASLARVGIRFWRTLEPGRMLAG
                     APMRLWRHPSWFEGASGPAPELLARSDRLIGIFHRACTARGARCAVLVLPDVRSLFEI
                     QTSGRNLLEVIYRRLPPPMTVWDPLPELSAAMAEKGVCHYVGNGRDCRGHFNADGNRL
                     IADFIARQLARLTGAP"
     gene            7740..8792
                     /locus_tag="PK264_13105"
     CDS             7740..8792
                     /locus_tag="PK264_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76852.1"
                     /translation="MTITRRTFGAGTAAAAAGLVLGSRAEAQGLAKDAETQLYEAAKK
                     EGQLTWYDAHHAVEQAEAYGKAFTAKYPGVKVNVVRTTANVAYQRFKQEVKAGGPQCD
                     VLSSTDISHPIEMKKLAQVEKFIPANAATLIPAVRNVDPDGMYHVTSIGTVGITLNSG
                     KVKEADAPKKWIDLVDPKWKNQVSVGHPGFSGYVSIWVWEMNRLYGWSYFEKLKANNP
                     QIGRSIQDTLTMLRAGERTVAAGSFATALEAKAKGEPIDAVYPEEGTIIIVAPSCIVK
                     GAKSPNAAKLFMEYMTSPASSEITVSYFGESIHDAVKAKVGKSLASVKTMIAPPEALS
                     KDLAGLKEKWRDTFGV"
     gene            8867..10591
                     /locus_tag="PK264_13110"
     CDS             8867..10591
                     /locus_tag="PK264_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRD76853.1"
                     /translation="MSDAAIPTSVGRAASRPRRSFDWTMLVWIAFAILLAVIVINPIL
                     RLVWESVATESGGITFDHYLGAYGRWRYIQSLLNTLVMGLGTATLATLIGVPLAWACV
                     RTDMPGRGFIKLCVLAAFIIPPYLAAVGWVLLAGPNAGWLNRGWMWLTGAKTGIFNIY
                     SVWGLIVVMGLHLFFFIFVFTSSALELVSSEMEDAANILGAGPVKTAFRVTLPLIMPA
                     ILGGFIIIFLQSIALFGVPAIIAIPARYPVVTTQLQEFFSSNNRVDLAAAYAIPLLLI
                     TMGMLGLQRWFLGRRGFTVVGGKGGERRVVRLGRWRWVMFAFALFVATLAVFMPLLVL
                     LQAAFAKAWGAGLSLDNLTLDHVRFILFKHETAVWSLLRSIIYAGAAAFSALTLALVI
                     GYVVKRGLMRFGNVLAFLCMAPFVIPGIILAIGFYAAYAPPPLMLAGTSLIIIVAFTT
                     RLLPIAYQNASAGIAAIHVEMEEAVRILGGNRLTAIRRVVAPLLSRSLIGAWILIFVP
                     ACQELSTAIFLSGPGNRVLSVLIYDFSEEGRFEQLAVLGGLLLIVTVAIVAIGFRIVG
                     RDFMIRRQ"
     gene            10591..11673
                     /locus_tag="PK264_13115"
     CDS             10591..11673
                     /locus_tag="PK264_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76854.1"
                     /translation="MPVVHLKGLTRTFGDFNAVDNVDLEIRDGEFLSLLGPSGCGKTT
                     TLRMIAGFIDPSGGTIDVDGEVLSSESGSLPPEKRGMSMIFQSYAIWPNMTVAQNVGF
                     GLDVRRVPRDEARRRVGEVLEVVRMGHLAGRYPAELSGGQQQRVALARAIVVNPSVLL
                     LDEPLSNLDANLREEMRFEIRRLHEQIKITTVYVTHDQSEAMATSDRIAVMNKGRIEQ
                     VDAPYTVYTRPRTRFVAGFIGRTNFMTAERRGDAFAFDGFAVPTARFSADDLARVSGR
                     SVTFSVRPQSVYLVRTPLAPGSGFVEVEGEIAHRTFLGEHWDYQFKPQGSEQRIKVAA
                     APADVFEVGTSARLALDPALMIPIVD"
     gene            complement(11746..>12013)
                     /locus_tag="PK264_13120"
     CDS             complement(11746..>12013)
                     /locus_tag="PK264_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76855.1"
                     /translation="FERALWFVFFGFLIFFFITFAFALTFVFGAILADFFFAIAGIAV
                     SLSVLRFRVSPRQSAQTNRYCLPESTISLIWLMSGKRNKKHGKK"
     assembly_gap    12014..12023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2064 a         3973 c         4003 g         2190 t
ORIGIN      
        1 cggctacacc aagctctaca tcgaccacgt caactcggcg agcgagggcg ccgacctcga
       61 cttcctggtc ggcaagtcgg gccgcgacgt gccgcgggac aatcattgag ctgatactgg
      121 aagcgaaatt gagtttcgag cggcggtcgc acaacagcgg ctgccgtttc gcttgcgtga
      181 gatcccgccg actgtaccgt tcatctgcca gtcagcatcc gtacacgccg ccgccccaat
      241 ctcaccccaa gcgtgcctaa gaggcggcct acagcctgtg gtcgaggccc gactgccgtc
      301 atgcggggtg gcaggacatg ggccgggcgg tgccggggtg ggatgagcgc agcgcaggcc
      361 gcgctgtggc gtccaggctc gacggcaacg cgagatccgg agaggcgggc tcgtcatggc
      421 gtcgacgtcg ggtgatacgg ctaggggcag gtcggcggac ggctgggacg cgatcgtcaa
      481 ggacgagcgg cccaagcgcg actggaagaa gctcctcctc gtcgtcggcc tcggcgtgct
      541 ctcgtgggtc gcgacctacg tcggcatgct cgaactgatc gaggccaaca tgggcgagct
      601 gccgctcgtc cataaaggca tcatcggctt ctcggtcgcc atgctgatgg tgatggtcgt
      661 gtggctgctc gaccagatct tcgcgccggg cgcgggtttc gtcgccaagc tctcgtatat
      721 tttcggctac gtcttcctga cgatcatctc ggtcggcttc ggcttcggct tctactggaa
      781 ggtgctcgag agccgctcgg agggcacgcg ctcggcggaa agcgccatcg gccaggtgca
      841 gaactcgttg catgcggcaa cgacgcgtct ggagcagctc gtcgcgacgc tcgaaagcct
      901 gacgctcctc tccacgcaga aggccgagtt cgagcgcgcg aatggcacgt catgtcccgc
      961 cagccggccc ggtgacggcc cgcgccgcaa gatgcgcgag gacgatgcgc agcgcttcac
     1021 gtttgcctcg gatttcgtca agggtcgcgt cgccaacgtg aaggccgaga tgaaggccct
     1081 cgacgtcgac ctcgccaaga tcgtccgcga cgacaagagc gcgatcgatg cgaagtcggg
     1141 aacgcgcaac gagttcatgc gtgcactcgg ccgcaagctc gagctgacgg tcacgggctt
     1201 caacgcgttt cgcggcgatc cacagctgcg ccagatccgg caggacctcg ccgaacgggg
     1261 cgagcagtcg tcctttccgg atggcaaggg gaagacgttt gcctgccccg atggccaatt
     1321 gcaggtggcg ctgcgcggcg tggtgcgcgc gatcgaccag ctccccgagc tcgagcggcc
     1381 gaagatcgcg gtcgtcgaag gttcggaagc ggtgatcgag gcgttccggc ggctgacggc
     1441 gacgttctac ggcgcgctcg ccttcaagct gccgccgagc cccgaggagt tgcgcgagct
     1501 gcagaagaaa gcgatcgctg ccgcagagag tggggcgacg ggcgccgccg cgcgcatccc
     1561 gagcctcgag caggcggggc tctcgaagcg cgactacatc ccgctcgcga tcgcggtgtt
     1621 cgtcgacctc tgcctgctgc tcgtctcgat gggccggccg agcaaccggt tgaacagcct
     1681 cgttcccaag atgcgggcgg ctgagcgcgg gccggtttat cagatcctct cgcgcttcaa
     1741 ccagatccat cacgacggcg aaatgcgcca gcacttcgag ctcttccgcc acgtcgtgtt
     1801 cgactacttg aacgactatt acgtcgcggt gccgctcgac gcgccgaagg tggtcttcga
     1861 ctggctgccg gtgccgaagg acaaggtgcg caccacgatg gagcatcgcc agcgcatgct
     1921 ctcgactgcc gagcgcgccg agctgcagcg cgaggcgcat cttctcgcca acctgttcgc
     1981 gagcttcgag caggagaaga tcttcttccg catcgatgat cgctggctcg gccgcatgac
     2041 gctgcaggtg ccgacctcag aacaggcgcg caagaagctc cgccgccaag gctcgaagtt
     2101 cgccgaggcg gaagctttcc gcatctacaa gttctcggat ggcgcctggt ccgagatcat
     2161 tctcggcgcc gtgatgggag ctgcccgccg tgtcgaggcg cgccgcgtcg ccagcggcga
     2221 gccggagccg gtgctgccga tgccgtccgc gcgtacacag ccgatgccgg tcgcggaacc
     2281 gggggacgtt ctggcgcacg atccgctctc agccgtccgt cctttcgcga tgcgcccgcc
     2341 ggtgcggccc gcgagcgaga agatcgagcc gacgctcgcc tcagttgccg ccaccgggac
     2401 gcgtcgccag ccacggaccg tcgtccccga cgagcgccat gatgcccagt tcggacccta
     2461 cgcgcgctat gccgagcctg ccttcgagga cgcgtatgtc ggcgagtccg cgaacaacaa
     2521 cacggcgcca aatccgaagc cagtcgtcgg cgtgacggcc gatggcgcgc gcgctgtgca
     2581 gccaagcccg ctgccaccag ccgatatcct tccggcgagc gtgccgacgg ctgcgagcgt
     2641 gtcagcaacg acggccggcg cggctgtcgg ctcgctgctc cgccgcctcg cagacacccc
     2701 tcgagcgacc cgcacaggag cggtcgaacc ggacacgatg accgtcgagc tcgtcgagcg
     2761 gactgcgacg gtgcatgtcc cgaaggaagg cacgctctcg gacgtcgtgc agcgcgcact
     2821 gaagacggcg aaagcggcac atgtcgagac cgttgcgccg acggtgatcg cgcatcagcc
     2881 ggatgtcgcg cccggaccgt cagagaccgt gcatgaccag atgctggccg tgatcaaggc
     2941 cgctgaggcg gccccggttt tcgaggtcga ggcgcctcac gccggcgata cgagtgaccc
     3001 ggaaatggcg cggctctcgg cgcggtttgc ggcgcgggtg gcgccgtagg ctcggaccgt
     3061 caatgaattg ggccgaggat cggcgggctc aaggcccgcc gatgtcgatt gcgatcactc
     3121 gctccggacc gccaggagat ggcggagatg gtatcagacc catgcgtctg acaccttgca
     3181 gcgacacctg gcatctgccg tcgaggtgaa cgggtgatga gatcgaagca accgcgtcgc
     3241 ggcagagcaa acagtcggaa tcgaaatgtc ctggccgtca gccgcggccg ggcagcttcg
     3301 gcttgccgac gataacggtg atcatgttct cgggcttcag gaggcgcgcc gcgacacgct
     3361 tgatgtcggc gacggtgacg gcctcgatca gcttgttgcg cttatcgacg tagtcgatgc
     3421 cgagatcgtc gtagaggaca ccgagcagaa cgttggcgat cttggcattg gtgtcgaagc
     3481 gcagcgggta ggagccgatc agatagctct tgatgtcggc gagttccttc tcggtcgggc
     3541 cttcggccat gcgcttcaac tcggcgcgga tgatgtcgat cgactgcccg acggattcgt
     3601 tcttggtcgc aacacggccg ccgaatgagt tcgaggcgcg cagcgtctgc agatagctgc
     3661 tgacgccgta ggcgaggccg cgcttctcgc gcacctcctc catgaggcgg gtgtggaagc
     3721 caccgccgcc gaggatctcg ttcatgacga acgccgggat gaagtccggg tcgttgcgcg
     3781 ggatgcccgc catgccgaac atcacgaccg actgcggcac gtcgaactcg acgatcttct
     3841 ccttggcgcc ggagggaatg gtgatcggcg gaatgggcgt gatgttggcc ttgacggcaa
     3901 gctcgccgaa caccttgtcg aggagcaacc cgaagtcctt ggcactgata tcgcctacgg
     3961 cgaccagctt ctcacgcgcg aacaccttgc ggcgcaccgt ctcgatgtcg tcgcgggtga
     4021 tggcggcgac cgacttctcg gtgacgaggc ggctgtagag gtggttcgga taggcgagca
     4081 tcgcccactc tcggccagcg atcgtgctcg ggttcttcag ggcgtaggcg aggttcgcga
     4141 gcttctgctt gcgcatgcgc tcgatcgcct cggcatcgag ccggggcttt gcgagggcga
     4201 gccgcagcat ctcgaccgag gcgtcgcgat tggcggtgag cgactggaag gtgccgtaga
     4261 aggcatcgtc cgtcgcttcg aaagacatgc gcatggcaag ctcttccatg cgctcctggt
     4321 acttctgggc atctagatcg ccggcacctt cgtcgagcag cgtcgccaga agctcggcga
     4381 cgccttcctt gccgatcggg tcctccgagc ctccgccctc gaacacgaag cggaatgcaa
     4441 ggagcggcac cttccgctcc tcgacgagcc agcccttgat gccgccgggg cttgtgatct
     4501 cttggatctt catcgggctc gccagcgttg tcgtgggggg aaggaggaga gaggccagag
     4561 ccgcggcggc gatcgcgaga gcgccacgac gggtcgcttg ggtcttgacg tgcatgggtc
     4621 tatgtctccg acgacaagct caactgcggt tcttgggtgg gaccgccggg tcgacgcgcc
     4681 gcgcatccgg gctcgccggc tcgggcagca tggtgccggt gacggagcgg cggatatcga
     4741 ggtacttcgc cgccaccttc ttgatgtcgt cgagtgtcac gcggttgatc cgcgctggcc
     4801 attcctcgac gtccttcaat gtgcgcccga cggtgattgc ccagccgtac atctgtgcga
     4861 gggagtactg attgtcgctg aggtacacgt aatcggccat gtagagcgtc ttggcgcgtg
     4921 caagctcggc ctcggtgatg cccttggcgc ggatgtcggc gatgagctcg tcgaggctcg
     4981 cctcggcgcg tgacaacggc acaccgtcag cggcaacgac cgtgaacgag atcttgccgc
     5041 tgtcgcggcc atagccgccg tagccgccgc cggcattggc cgcgatacgc tcgtcggcga
     5101 cgagcttttt ataaagacgg ctggtgccgc cggacgccat gatcttcatg agcagctcga
     5161 gcgcctcggc ctcacctgcc tcggccgagg cgtagcttgg ggcgaggtaa tagcgatgca
     5221 gcgagtagcg accggcgcgc ggatcgcgga gttcgacgcg gcgggcaacg cggtgcaacg
     5281 gctcgggcgc gcgggggcgg gccatgacgg cggggttcgc cggcacgagg ccatacgtct
     5341 tctcggcgag cgctttcacg tgctcgggcg tgacgtcacc agagacgacc aggatcgcgt
     5401 tgttcggcgc gtagaagcgc ttgtagaagt cgatcgcgtt ctgcggcgtc agcctcgcga
     5461 tctcgttctc ccagccgatc acgggctgac catagggatg gacaaggtag agggcggcgt
     5521 ccatctgctc gccgaggatg gagccgggct tagactcgac cgtcgtgcgc cgctcctcga
     5581 ggatgacatc gcgctcggtc agcacttcct tctcggtcag gcggagattg accatgcggt
     5641 cggcctccat gcccattacc ttctccaggc gatccttgtg cacgcgctgg aaataggccg
     5701 tgacgtcctg gcccgtgaag gcattgtcgt tgccgccgag ccgcgaaaca atcttcgaga
     5761 actcgccgac ggcgatcttc tcggtcgact tgaacatcag gtgctcgagg aagtgggcga
     5821 cgccagacat gccggccggc tcatcggccc gaccgacgcg ataccagacc atgtgcgtta
     5881 cgaccggcgc ccggttgtcc ggaatgacga tgatctgcat gccgttggcg agccgcatct
     5941 cggtcgcctt gacttgactc caagcggagc cgggtccggt gagtgtggcg agaagtgtca
     6001 tcaggaagac gagccccatg gttcggctga ggttcgagcg tggatgcagt cggcgcatag
     6061 agcatcctca ggtcgtggag ggattggcgg gcaacggtcg gtgacatccg acgcgacgcc
     6121 gatggcatgg tggtgaccac actgtggcaa ggcgcgcaaa cgtccggagg cgaatgttgg
     6181 gtgttcgcct tgccaagtca aatgacaagc aatccatctg ccgatccatc tgctccaggt
     6241 gatcaatgat cacattttta ccatttacca ttaagagcac ttgcgcctct tgttccggat
     6301 cggagagtgc cgagcttgag cggttccgcc gcacctcatg tatcgcaggc gcccatcgca
     6361 tcatggacga tcccctcaag gtcacgccgg ctcagccgga tcgcccccga gcccggcggt
     6421 ggggtgcgcg tctgctgatc cggctttttc tgatggtcct cgcgatcgaa gggctaagct
     6481 acgcgctcct ccgcttcggc ccgcaagccc tgattcagac cgtctatcat ccgccggtga
     6541 tcggacgggc agcgttcgag acgttcatgg cggagcggca cccgatcctg tcttggccga
     6601 cccgggggcg cgacggccgc gaccgggaga ttccgctcga cgagcgtggc gcgcgcacat
     6661 cgatgaccaa tgtggtcttc tcgaatggcg atacctgcct ctcggcctac ggcgacgggg
     6721 ctgtgcgggc ggacggcgtc gccgaccatc acgcctggag cgatcttctt gcgcggcgcc
     6781 tcgcttgccg cgtcgacaac ttcggcatca gcggctacgg cgccgatcag gctgtgctgc
     6841 gtctcctgct aaaccccgca gatgcggcca aggccatggt tctcggcgtc agtccagcgg
     6901 cgctgctgcg gatgggcagc cagtggccgt tcctgctcga tgggccgagc gccggcctcg
     6961 cgatcctgac cgacatgacg ggacgcctcg acctcgtccc ggcacatgat ctcacctacg
     7021 agggctttct cggacttgcg cgcgatccgg cgctctatct cggccatgag cgctatctgc
     7081 cgggctcgcc gtatgggccg gagccgttcg cgtttccgta ttccgcaagc ctcgcgcgcg
     7141 tcggcatcag gttctggcgc acgctcgagc ctggccgcat gctggcaggc gcaccgatgc
     7201 gcctctggcg gcatccgagc tggttcgagg gcgcctcggg tccggcacca gagctgctcg
     7261 cgcggagcga caggctgatc ggcatctttc atcgcgcctg caccgctcgc ggcgcgcgct
     7321 gcgcagtgtt ggtgctcccg gatgtccgtt cgctcttcga gatccaaacg agcggtcgca
     7381 acctgctcga ggtcatctac aggcggctgc cgccgcccat gaccgtctgg gacccgctgc
     7441 ccgagctctc cgccgccatg gccgagaagg gggtctgcca ctacgtcggc aatggtcgcg
     7501 actgccgcgg ccatttcaat gccgacggca accgcctgat cgccgatttc atcgcgcgac
     7561 agctcgccag actgacgggc gcgccctgat cctaccgccg gtgaggatcg cgtggcagcg
     7621 atggttctgc cgaagccgac actgcttgta cggtcgggtg tccgtgctaa gctttcgcgg
     7681 tgctaccggt cgaaagctgc caatcacaga acaatcgttc ctcggggaga ggcgccacca
     7741 tgacaatcac acgcaggacg ttcggggccg gcacggccgc agccgctgcg gggctggtgc
     7801 tcggctcgcg ggcggaggcg cagggcctcg ccaaggatgc cgagacgcag ctctacgagg
     7861 cagcgaagaa ggaaggccag ctcacctggt acgacgccca ccacgccgtc gagcaggcgg
     7921 aggcctacgg gaaggcgttc accgccaagt accccggcgt gaaggtcaac gtggtacgca
     7981 ccactgcgaa cgtcgcctac cagcgcttca agcaggaggt gaaggcgggc ggcccacagt
     8041 gcgacgtgct ctcctcgacc gacatcagtc atccgatcga gatgaagaag ctcgcccagg
     8101 tcgagaaatt catccctgcg aatgccgcga cgctcattcc ggcagtgcgc aacgtcgacc
     8161 cggacggcat gtatcacgtg acctccatcg gcacggtcgg catcacgctc aacagcggca
     8221 aggtgaagga ggccgacgcg cccaagaagt ggatcgacct cgtcgacccg aagtggaaga
     8281 atcaggtctc ggtcggccat cccggcttct cgggctatgt cagcatctgg gtgtgggaga
     8341 tgaaccggct ctacggctgg agctacttcg agaagctcaa ggccaacaat ccgcagatcg
     8401 gccgttccat tcaggacacg ctcaccatgc tgcgcgccgg cgagcggacc gtggcggcag
     8461 gctcatttgc gacagcgcta gaggccaagg ccaagggtga gccgatcgat gccgtctacc
     8521 ccgaggaggg gacgatcatc atcgttgcgc cgtcctgcat cgtgaagggg gcaaagagcc
     8581 cgaacgccgc gaagctcttc atggagtaca tgacctcgcc cgcctcgagc gagatcaccg
     8641 tctcctattt cggcgagtcg atccacgatg cggtgaaggc caaggtcggc aagtcgctcg
     8701 cctcggtgaa gaccatgatc gcgccgcccg aggcgctgtc gaaggacctc gccggcctga
     8761 aggagaaatg gcgcgatacc ttcggcgtgt gagccgagac gcatgaaggg cgctcaccgt
     8821 gcgcccttcg aggttcggcc gtccaggtct cgagcagggc tttcccatga gcgacgccgc
     8881 catcccaacg agcgtcggtc gcgctgcatc gcggccgagg cgcagcttcg actggaccat
     8941 gctcgtgtgg atcgcgtttg cgatcctgct cgccgtcatc gtcatcaatc cgatcctgag
     9001 gctggtctgg gagagcgtgg cgaccgagtc gggcggtatc acctttgatc actacctcgg
     9061 cgcctatggc cgctggcgct acatccagtc gctgctcaat acgctcgtca tggggcttgg
     9121 cacggcaacc ctcgccacgc tcattggcgt gccgctcgcc tgggcatgcg tgcgcacgga
     9181 catgcccggc cgcggcttca tcaagctctg cgtgctcgcg gcctttatca tcccgcctta
     9241 cctcgcggcc gtcggctggg tgctgctcgc cggtcccaac gccggctggc tcaaccgcgg
     9301 ctggatgtgg ctcaccggcg ccaagaccgg catattcaac atctatagtg tgtggggcct
     9361 catcgtcgtt atggggctgc acctcttctt cttcatattc gtgttcacga gctccgccct
     9421 cgagctcgtc tcctccgaga tggaggatgc ggcgaacatc ctcggtgcgg gccctgtcaa
     9481 gacggcattc cgcgtaacgc tcccgctgat catgccggcg atcctcggcg gcttcatcat
     9541 catcttcctg cagtcgatcg cgctcttcgg cgtgccggcg atcatcgcca tcccggcgcg
     9601 ctaccctgtc gtgacgacgc agctccagga gttcttctcg agcaacaatc gcgtcgatct
     9661 cgccgcagcc tacgccattc cgctgctgct catcaccatg gggatgctcg gcctgcagcg
     9721 ctggttcctc ggccggcgcg gcttcacggt ggtcggaggt aaaggcggcg agcgtcgcgt
     9781 ggtgcggctc gggcgctggc gctgggtgat gttcgccttc gcgctgttcg tggcgaccct
     9841 tgccgtcttc atgccgctcc tggtcctgct gcaagcggcc ttcgcgaagg cctggggcgc
     9901 cggtctctcg ctcgacaacc tcacgctcga tcacgtgcgc ttcatcctct tcaagcacga
     9961 gacggccgtc tggtcgctgc tgcgctcgat catctatgcg ggtgccgccg cattctcagc
    10021 cctgaccctc gcgctggtga tcggctacgt cgtcaagcgc ggcctcatgc gcttcggcaa
    10081 tgtcctcgcc ttcctgtgca tggcgccgtt cgtcattccc gggatcatcc tggctatcgg
    10141 cttctatgca gcctacgcgc cgccaccact gatgctcgcc ggcacgtcgc tcatcatcat
    10201 cgtcgccttc acgacgcggc tcctgccgat cgcctaccag aacgcctcag ccgggattgc
    10261 cgcgatccac gtcgagatgg aggaggcagt gcgcatcctc ggcggcaacc ggctgacagc
    10321 gatccggcgc gttgtagcgc cgctactcag ccgctcgctg atcggcgcct ggatcctcat
    10381 cttcgtgccg gcctgccagg agctctcgac ggcgatcttc ctgtcggggc ccggcaatcg
    10441 cgtcctctcg gtgctgatct acgacttctc cgaggagggg cgcttcgagc agctcgcggt
    10501 gctcggcggc ctgctgctca tcgtcaccgt tgccatcgtc gccatcggct tccggatcgt
    10561 cggccgcgat ttcatgatca ggagacagta atgccggtcg tgcatctcaa gggcctgacg
    10621 cgcacgttcg gcgacttcaa tgcggtcgac aacgtggatc tcgagatccg cgacggcgag
    10681 ttcctgtcgc tgctcggtcc ctcgggctgc ggcaaaacga cgacgctgcg catgatcgca
    10741 ggcttcatcg acccctcggg cggcaccatc gacgtggacg gagaggtgct ctcctcggag
    10801 agcggctcgt tgccgcccga gaagcgcggc atgtccatga tcttccagag ctatgcgatc
    10861 tggccgaata tgaccgtcgc ccagaacgtc ggcttcggcc tcgacgtgcg cagggtgccg
    10921 cgcgatgagg cccgccggcg ggtcggcgag gtgctcgagg tcgtccgcat gggacatctc
    10981 gccggccgct acccggcgga gctctccggc ggccagcagc agcgcgtggc gctcgctcga
    11041 gcgatcgtcg tcaatccgtc ggtactgctg ctcgacgagc ccctgtccaa cctcgatgcc
    11101 aacctgcgcg aagagatgcg cttcgagatc cgccggctgc acgagcagat caagatcacc
    11161 accgtctacg tgacgcacga ccagtccgag gcgatggcga catcggaccg gatcgcggtc
    11221 atgaacaagg gccgcatcga gcaggtggac gcgccctaca ccgtctacac gcggccgcgg
    11281 acgcgcttcg tcgcgggctt catcggccgc acgaacttca tgacggcgga gcgtcgcggg
    11341 gacgcattcg ccttcgacgg gttcgcagtt ccgacggccc gcttttcggc cgacgatctc
    11401 gcgcgcgtct caggccggag cgtcacgttt tcggtccgcc cgcaaagcgt ctacctcgtg
    11461 cgcacgccgc tcgcaccggg atcgggcttc gtcgaagtcg agggcgaaat cgctcatcgc
    11521 acgttccttg gcgagcactg ggattatcaa ttcaagccgc aaggaagcga gcagcggatc
    11581 aaggtcgcgg cggcacccgc ggatgtgttc gaggttggaa cgtcggcacg cctcgcgctc
    11641 gatcccgcac tgatgattcc gatcgtcgac tgagatggcg acggaaaaag cgttgcattt
    11701 tcagcgcagt ccagccgtcc gatctcatga aaatgagcgc gaggatcatt ttttcccgtg
    11761 ttttttattg cgtttaccgc tcattaacca gattaatgaa atcgtagatt cgggtaggca
    11821 ataccgattc gtctgggcag actgacgggg gcttacccga aaacgcaaaa ccgagaggga
    11881 gactgcaatg cctgcaatcg cgaagaagaa gtccgccaag atcgctccga agacgaaggt
    11941 caaggcaaag gcgaaggtga taaaaaagaa gatcagaaag ccgaagaaaa caaaccagag
    12001 agccctttca aacnnnnnnn nnnggcagcg aagcgctcag gcgccgcccg ccgctcgacg
    12061 gcgaaggccc gcaccacgac gaaggcccgt cccgccgcca agcgccgcac gaaggcctga
    12121 tcgaaactcg aaaccatccg atcggcaatc ggattacggc cggcagctct ccaggagcgc
    12181 cggccgaaat cttttgcggg tcctgcgtgg cactccatga taccggccaa ctgaaggttc
//
LOCUS       DAORNH010000122        12196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3933_length_12196_cov_2.521209, whole genome shotgun sequence.
ACCESSION   DAORNH010000122 DAORNH010000000
VERSION     DAORNH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_12196_cov_2.521209"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>361
                     /locus_tag="PK264_13125"
     CDS             <1..>361
                     /locus_tag="PK264_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HRD76856.1"
                     /translation="EGFDTVGVADLDALDARTAERLRAFLAEGRHGTMDWLAVRAEER
                     GHPRRLWPAARMAIMLGLNYGPDADPLARLGEPTRGVVSIYAKRRDYHDVVKGKLKQL
                     AQWLARESGADVKVFVDT"
     assembly_gap    362..382
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            383..1083
                     /gene="queG"
                     /locus_tag="PK264_13130"
                     /pseudo
     CDS             383..1083
                     /gene="queG"
                     /locus_tag="PK264_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041672.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
     gene            1177..1365
                     /locus_tag="PK264_13135"
     CDS             1177..1365
                     /locus_tag="PK264_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HRD76857.1"
                     /translation="MDWDDLKPKPRPAITLGQSLVDLSIAELEARVLALNEEIERVRA
                     EIAAKKAHATAAGSIFKS"
     gene            1717..2250
                     /locus_tag="PK264_13140"
     CDS             1717..2250
                     /locus_tag="PK264_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HRD76858.1"
                     /translation="MTVSFAERFASSDQFEQLFKDGMGLVEATASYLDGQGRRDARQL
                     TPGIALIYATESMRLTTRLLEIASWLLLRRALRDGEINESEARVRRRTIRLGTIGRPS
                     HVTGWAELPPELRRLVDASFAVNDRIVQVDKVLNGSASIEPTADQNPVAHRIAAIEQA
                     FGDQKVTIRKVSGGQRG"
     gene            complement(2369..2956)
                     /locus_tag="PK264_13145"
     CDS             complement(2369..2956)
                     /locus_tag="PK264_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRD76859.1"
                     /translation="MALPNFKSSEPKPLFDIASLAGASSAVSAGVVPPPLPASDSAAL
                     PVMMSEPAREAARSSAPAIKPKSVIGADLKIIGQQLTITSKAPLQIDGQIQGDVRGTE
                     VVIGAQGRVDGSIAAETIIVHGSASGIVRGVNVKLASGSRVEGDVHHMLLGVEQGAVF
                     EGRARRHGERRDLVPDLDQTGEIVPMPRVVGAARR"
     gene            complement(3127..4083)
                     /locus_tag="PK264_13150"
     CDS             complement(3127..4083)
                     /locus_tag="PK264_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76860.1"
                     /translation="MWRMRWPPVPQLVAAIGVVASVGAAECAVRFKRNSGMVAPPPAT
                     AAECERGGRVHVVVSEEAECVAYHAGAPLYGARKVVLFLDGDLPLPYQRSASLQRDYL
                     KRMLEILQILSRRSSGEVPFVLLARPGTFGSTGDHGKRRTEREIDVLNAAIDRLKARY
                     RVVSWVIAGQSGGATSLAGLLIRGRRDIECAIPGSGAFDYFGALAAWNELHGDEPLTS
                     DKFVAYRDRFHAMDNLDRIPRDPRRRIFVVGDPEDRVTPFRFQETFQKSVAAAGHHAV
                     LVPTLGAGEERHGVTYTVLRLAALCADGASDGTIERAAPRPR"
     gene            4275..9125
                     /locus_tag="PK264_13155"
     CDS             4275..9125
                     /locus_tag="PK264_13155"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HRD76861.1"
                     /translation="MAERPETMSSALTRAMEASPAGTTGGVELFARLLLASTDAQNLD
                     GIGEDGLAEIVQHGLRMIAEKPAHTHAVSISHVDARPGDVSRRGVTIIDVLNDDMPFL
                     VDSVMGELHARGPVVRLVLHPIFKTRRDGAGRLEEIVRPGDRNWNDGNQESYISVHVD
                     RLTDAAAADVRNALSSILADVRRAVTDWRAMIDRLGLEIARLEKRAGTQAGDHGEAIA
                     FLRWLQEGQFTFLGMRDLRLVGGQGESELETVADTGLGILRDPTIEVLRRGRDLVSMT
                     PEIRRFFLSPDPLIITKANVVARIHRRAHMDYIGIKSYDESGRPVGELRVVGLFTSRA
                     YTHSPSGIPLLRRKVEHILGALGYPPESHAGKALINTLETFPRDELFQVEAERLVEVA
                     RGILDLDLRPRVRVFTRVDRFDRFVSALVYVPRDRYTTSTREAIGELLARRFNGRVSA
                     SYPHVPGGPLARVHVIIGRSDGATPVVLERELEADIIALVRTWSDKLGELIASGEPGS
                     TGALAAKYGRAFSVGYAETFPPERAIEDIARIERLGAERPVAIDFYHEPSSASAAEGV
                     HRTHAAIYRFDQPIPLSERVPVLENLGFRVIDERTYRITPSFEAGQRVVALHDMVLEP
                     VGGAPMALDAHDQRLEDAFLAVLKGEADNDGYNRLIPLAGAGWQEAGLLRAYGAYLRQ
                     LAVPFGQRYLADVMTLHPGIARLLLDLFRARLDPDLAMTGDARRSEVARIEAALQQAL
                     GKVASLDEDRILRLFLSAITATVRTNVYLRGPDGRAPETIALKLKPREIEAAPQPRPF
                     AEIWVFSPRLDALHLRFAPIARGGIRWSDRPQDFRTEVLGLCKAQQVKNAVIVPAGAK
                     GGFVPKLIPRNATRDVIQREGVAVYETFVRAALSITDNIVDGKVLPPERVVRHDGDDP
                     YFVVAADKGTATFSDMANAISLERGFWLGDAFASGGSAGYDHKKMAITARGGWECVKR
                     HFREMDRDIQREPFTAAGVGDMSGDVFGNAMLLSPATRLIAAFDHRDIFVDPDPDAAV
                     SFAERKRLFDLPRSSWADYNRKLISKGGGVYSRAAKSIELAPEARQRLGIAATAVTPA
                     ELMRHILLLDVDLVWFGGIGTYVRATSENDLDVGDRGNDAIRITGRELRAKVIGEGAN
                     LAMTQRGRIEYALRGGRLNTDFIDNSAGVNSSDQEVNIKIALGPVTRSGRLDPEARKA
                     FLAGMTDSVAAACLRNNYQQSLALSLAERNSAADMAFNARLIRALAARGMLERRLESL
                     PTDLELGERQKAGQGLTRPELAILLSYSKIALSHDLLASGAPDEAMVSNLLEGYPPRA
                     LANAYHAELRVHPLRREIIATGLTNLVVNRCGPSAPVRLADETGRSSVDVALAIFAAR
                     SILDLPSLWERIDALDAKVAGNAQLDLYARVQEALIRAATTLLRQGDASTLGARIETH
                     HAAASELAGLERGELPTGTRTSLEKDVREMTTVGLPVELAERVAALRLSSEASAVAEL
                     ARTAGASIAETARVRLEISDHLQLGAILDKARSVPAPDYYDRLAVASAEAALLDAQRM
                     MTIAAIDAGRKPGLTGSAFAAWVASGGKGLEGTRGRLAEIASGSGLSVSRLTVAAAQA
                     REAATG"
     gene            complement(9177..9782)
                     /locus_tag="PK264_13160"
     CDS             complement(9177..9782)
                     /locus_tag="PK264_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD76862.1"
                     /translation="MKLLSSPLSPYGRKVKMTALMKGVMGDIELVPADTNKGDNTLNA
                     QNPLGKIPCLIPAGGSAIYDSHVICEYLDSLKPAPALFPKSGTERWKTLTMGALGDGI
                     LDAALLLVYEKRFRPETMLVQAWVDRQQAKIDRALGQLEKAPPAYGNSPDYGHVTIAA
                     ALGYLDFRHEGKWRKSHPKLVAWLDGFAKVVPAFGETKPVA"
     gene            9956..11140
                     /locus_tag="PK264_13165"
     CDS             9956..11140
                     /locus_tag="PK264_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRD76863.1"
                     /translation="MTSLASVSKGTPPFKTDELDRQMVAAGLDIIVATSRHNVQYLLG
                     GYRFFFFETMEAMGTSRYLPAVIYRCGRPAETVYVGCGMESFERDNGRLWPQTLDLGA
                     TGSEATARQVVGHIERLGGEGASIGLELPFIPADAAHVIRQGLARSTVLDATVPLERL
                     RAVKTPAEIGMLRHASEQVVDAILATFRAVKPGQTKSEISDRLRREEVQRGLVFDYCL
                     ITAGTSLNRAPSPQAIKAGDIISLDSGANNNGYIGDLCRMGILGRPDSELVDLLALVE
                     EVQQAARRVVRAGTRGGEVVAVGEAMVKRSPHAAYTHYMAHGMGLVSHEAPRLATMPR
                     FSYSGDDASKPLAAGMVLSIETTMAHPRCGFIKLEDTILVTKDGHEALGDGARGWNVI
                     PL"
     gene            11233..11604
                     /locus_tag="PK264_13170"
     CDS             11233..11604
                     /locus_tag="PK264_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76864.1"
                     /translation="MLDQIEHAASVSIARACGFAMLGIATFLIGLSAVETTLMLKTGG
                     ILTLIVCLALVLKASLAAGQPYKSTEVWIMLPPEHRPGAAIAQQVIGTVLRETYLRFA
                     IYASLLSAALFAAALVSGLAQ"
     assembly_gap    11799..11926
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11927..>12196
                     /locus_tag="PK264_13175"
     CDS             <11927..>12196
                     /locus_tag="PK264_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRD76865.1"
                     /translation="HMTIQTAVLIETLAALGADIRWASCNIYSTQDHAASAIAAAGIP
                     VFAVKGESLVEYWDYTRKILEWSDGGTPNMILDDGGDATMLVHYG"
BASE COUNT         1990 a         4033 c         4003 g         2021 t
ORIGIN      
        1 ccgagggctt cgataccgtc ggcgtcgcgg atctcgacgc cctcgacgca cgaacggcgg
       61 agcggctccg cgcgttcctc gccgagggac gccacggcac gatggattgg ctcgcggtgc
      121 gggcggagga gcgcggccac ccgcgccggc tctggcccgc ggcgcgcatg gccatcatgc
      181 tcggcctcaa ctacgggccc gacgccgatc cccttgcgcg actgggcgag ccgacgcgcg
      241 gcgtcgtctc gatctatgcg aagcggcgcg actatcacga tgtcgtcaaa ggcaaactga
      301 agcagctcgc gcagtggctc gcgcgcgaga gtggcgctga cgtcaaagtg ttcgtcgata
      361 cnnnnnnnnn nnnnnnnnnn nnccgagcgc gccgggatcg gctggcaggg caagcacacg
      421 aacctcgtct cgcgcgcgct cggctcgtgg ctgttcctcg gcgcaatcct gacgacggcc
      481 gagatcgagc ccgatctgcc cgaatccgat cactgcggca actgccggcg ctgcctcgac
      541 atctgcccga cagatgcgtt cccggcgccc ttccagctcg acgcgcgccg ctgcatctcc
      601 tatctcacca tcgagcatcg aggctcgata cccgtcccgc tcaggtccgg catggggaac
      661 cggatattcg gctgcgacga ctgcctcgcc gtctgtccgt ggaacaagtt cgcggcggcg
      721 gcgcgcgaga gccgactcgc cctcaatgcg ctcgccgatg atcccccgct cgccgagctg
      781 ctcgccctcg acgaccaggc cttcagggcg aggttcgcgg ggacgcccat aaggcgctcc
      841 ggccgcgagc ggatcatgcg aaattgcctg atcgcggccg ggaactcagg cgaccccgct
      901 cttgcccggc acgtcgagcc gctgcttgcc gatcccgcgc cggtgatccg gggggcggct
      961 gtgtgggcga tcgcgcggct tctcagtgct cctcgcgttc gcgaccatgc cgaccgattg
     1021 cggccgggag agcgcgatcc ggaagtgctc gccgaatggg acctggcgct cgccgcgatg
     1081 taagcggcga ccgttcagcc gctcagcgcg cttcccgagc ggcgcaccat gtggcagctt
     1141 cggcaacgtc tccggagggc atgcaggagg agcgccatgg actgggacga cctcaagccg
     1201 aagccgcggc cggcgatcac gctcggccag agccttgtcg atctctcgat tgcggagctc
     1261 gaggcgcgcg ttctcgcgct caacgaggag atcgagcggg tcagggccga gattgccgcc
     1321 aagaaagcac atgcaaccgc cgccggctcg attttcaaga gttgaggggt ggccgaatag
     1381 ttcgggctag gtaaatcgtg aattcgataa agagcctggc cgaattaact tggcgttaac
     1441 caattcgacc taaattgaca cgtatccagt catctggatg cgagtggctc ctgtccactc
     1501 tgtttgacgc ctccctgtca actcttgagc cgctgttcgc agcggctctt tttttggccg
     1561 gtggccacaa aatgcgctca ttatgggtcg aggtattaag cctgatcggc aatggttaat
     1621 tggcctgatc attgacgggg tattgtcgcc cgtgcgttgt cggtacagcg gggttgtggg
     1681 atgtctggag ttgtgagtgc aatggggtcg cgtggcgtga ctgtctcatt tgccgagcgc
     1741 tttgcgtcgt cggatcagtt cgagcagctg ttcaaggacg gcatggggct cgtcgaggcg
     1801 accgccagct atctcgacgg tcagggccgt cgtgacgcgc gtcagttgac gcccggcatc
     1861 gcgttgatct atgcaaccga gagcatgcgg ctgacgacgc gcctcctcga gattgcctcg
     1921 tggctgctgc tgcggcgggc cctgcgggat ggcgagatta acgaatccga ggcgcgcgtg
     1981 cgccgccgca cgatccgcct cgggacgatc gggcgcccct cacacgtgac cgggtgggca
     2041 gagttgccgc ccgaactccg gcgtctggtc gacgcgagct tcgccgtcaa tgatcgcatt
     2101 gtccaggtcg acaaagtgct gaacgggtca gcgagcatag agccgactgc tgatcagaac
     2161 ccggtcgccc acaggattgc tgctatcgag caggccttcg gcgaccagaa ggtcaccatc
     2221 cgcaaggtat caggcgggca acgcggctga ggcgacaccg ccctgcatta ccctgaaagc
     2281 cccgagttcc gaatccaaca gaaaacgccc gcgaggttca cgggcgtttt ctgtttcgca
     2341 gcgggatgcg attgattgcg accggaaatc aacgacgtgc ggcgccgacc acacgcggca
     2401 tcggcacgat ttcgcccgtc tggtcgagat cgggaacgag gtcacggcgc tcgccgtggc
     2461 ggcgcgcgcg gccctcgaag acggcgccct gctcgacgcc gagcagcatg tgatggacat
     2521 cgccttcgac gcggctgccg gacgcgagct tcacgttgac gccgcggaca atgccgctcg
     2581 ccgagccatg cacgatgatc gtttcggcgg cgatgctgcc gtcaacgcgg ccctgcgcgc
     2641 cgattactac ttccgtgcca cgcacatcgc cctggatttg gccatcgatc tgcagcggcg
     2701 ccttcgacgt gatggtcagc tgctggccga tgatcttgag atcggcgccg atgacggact
     2761 tcggcttgat cgccggcgcg ctgctgcggg ctgcctccct tgctggctcg ctcatcatga
     2821 cgggcagtgc agccgagtcg gacgccggca gcggcggcgg cacaacgccc gcgctgaccg
     2881 ccgatgatgc tcccgcaagc gaagcgatgt cgaacagggg cttcggctcg gacgacttga
     2941 agttcggtaa cgccatactg acacctcttg actgggggac gcatccgtaa ggcgccactc
     3001 cgcgatggtc tcggcatggt cgcaatgtga cagaactaag tttttccacg aattggtgtt
     3061 cccacgacat agatggcctc gcccacagca caagtgatgt gcgagttgct gccctatccc
     3121 acaacctcaa cgcggtctcg gagcggcccg ctcgatggtg ccatcgctcg cgccatccgc
     3181 acagagcgct gcgagccgga gcaccgtgta cgtgacgccg tggcgctcct cgccggcccc
     3241 gagcgtcggc acgaggacgg cgtgatggcc cgccgcggcg acacttttct ggaatgtttc
     3301 ctggaagcgg aacggcgtca cgcgatcctc aggatcgccg acgacgaaga tgcgccgcct
     3361 ggggtcgcga gggatccggt cgagattgtc catggcgtgg aatcggtcgc gataggcaac
     3421 gaacttatcg ctggtcagcg gctcgtcacc atggagctcg ttccacgcgg cgagggcgcc
     3481 gaagtagtcg aaggcgccgg agcctggaat ggcgcactcg atgtcgcggc gtccgcggat
     3541 gagaaggccg gccagcgatg tggcgccacc ggactgtcct gcgatcaccc agctcacgac
     3601 gcggtagcgc gccttcagcc gatcgatggc tgcattcagt acgtcgattt cgcgctctgt
     3661 ccgccgcttg ccgtgatcgc ctgtcgagcc gaaggtcccg ggccgcgcca gcagcacgaa
     3721 gggcacctcg ccgctgctgc gccgcgagag gatttgcagg atctccaaca tgcgcttcag
     3781 gtagtcgcgc tgcaggctcg cggagcgctg gtagggaagc ggcaggtcgc cgtcgaggaa
     3841 cagcacgact tttcgggcgc cgtagagcgg ggcgccggcg tggtacgcga cgcactcggc
     3901 ttcttccgat acgacgacgt gtacgcgacc tccgcgctcg cactcggcgg ctgtcgccgg
     3961 tggcggggcg accataccgc tgttgcgctt gaaccggacc gcgcactcgg cggcgcccac
     4021 agaggcaacc accccgatgg cggcgaccag ctgcggaacc ggcggccatc gcatgcgcca
     4081 catatcagag ctccgatccg gatctgatgt cccgttcacc atgcagcacg catcccctgt
     4141 cgtcgagatc gcgtgcaatc gtcggcgatc cgggttgccg acaagctacc aagacgcaag
     4201 ggatacatta ccgtgtggcg atgaataggc tacgctccga cgagccgttc atcgttcgag
     4261 agaggcaggt ggccatggca gaacgccccg agaccatgtc gagtgcgctc acccgcgcga
     4321 tggaggcaag cccggccggt acgacaggcg gagtcgagct gtttgctcgg ctgcttctcg
     4381 cgagcacgga tgcccagaac ctcgacggca ttggcgagga cgggctcgcg gagatcgtcc
     4441 agcacggcct ccggatgatc gccgagaagc ccgcgcacac ccatgccgta tcgatctcgc
     4501 acgtcgatgc gcgccccggt gacgtgagcc ggcgcggcgt cacgatcatc gacgtgctca
     4561 acgacgacat gccgttcctg gtcgattccg tgatgggcga gctgcacgcc cgtggcccag
     4621 tcgtccgcct cgtactgcat ccgatcttca agacgcgccg cgatggcgcc ggccggctcg
     4681 aggagatcgt caggccgggt gaccgcaact ggaacgacgg gaaccaggaa agctacatct
     4741 cggtgcatgt cgaccggctc actgatgcgg cggccgcgga tgtgcgcaac gcgctctcct
     4801 cgatcctcgc cgacgtgcgc cgcgccgtca ccgactggcg cgcgatgatc gatcggctcg
     4861 gtctcgagat cgcgaggctc gagaagcgtg ccggtacgca ggcgggcgac cacggcgagg
     4921 cgatcgcctt cctgcgctgg ctgcaggagg gtcagttcac gtttctgggc atgcgcgacc
     4981 tgcgcctcgt cggcgggcag ggcgagagcg agctcgagac cgtcgccgac acgggacttg
     5041 gcattctgcg cgatccgacc atcgaggtcc tgcggcgcgg gcgcgacctc gtctcgatga
     5101 cgccggagat ccgccgcttc ttcctctcgc ccgacccgct gatcatcacc aaggccaatg
     5161 tcgtcgcgcg catccatcgc cgcgcgcaca tggattacat cggcatcaag agctacgacg
     5221 agagcggtcg gccggtcggc gagttgcggg tcgtcgggct gtttacctcg cgcgcctata
     5281 cgcattcgcc gtcgggcatc ccgctcctgc gacgcaaggt cgagcatatc ctcggcgcgc
     5341 tcggctatcc gccggagagc cacgccggca aggcgctcat caacacgctc gagacgtttc
     5401 cccgcgacga gctgttccag gtcgaggccg agcggctcgt cgaggtcgcg cgcggcattc
     5461 tcgacctcga tctgcgcccg cgcgtgcgcg tcttcacgcg tgtcgatcgc ttcgatcgct
     5521 tcgtctcggc gctcgtctac gtgccccgcg accgctacac gacgtcgacg cgcgaggcga
     5581 tcggcgagct cctggcccgc cgcttcaatg gacgcgtctc cgcatcctat ccgcatgttc
     5641 ccggcggtcc gctcgcgcgc gtgcacgtga tcatcggccg ctcggacggg gcgacgcccg
     5701 tggtcttaga gcgcgagctc gaggccgata ttatagccct cgtccgcacc tggtcagaca
     5761 agctcggcga gctcatcgcc tcgggcgaac cgggcagcac gggtgcgctt gccgcgaagt
     5821 acgggcgcgc gttcagtgtg ggatatgccg agacgttccc gcccgagcgg gcgatcgagg
     5881 acatcgcgcg gatcgagcga ctgggggccg agcgtcccgt cgccatcgac ttctaccatg
     5941 agccgtcgag cgcgtcggcg gcggaagggg tgcaccgcac gcacgccgcg atctaccgct
     6001 tcgatcagcc gatccccctc tccgaacgtg tgccggtgct cgagaacctc ggcttccgcg
     6061 tcatcgacga gcgcacctat cggatcacgc catcgttcga ggccggccaa cgggtggtcg
     6121 cgctccacga catggtgctc gagcctgtcg gcggcgcgcc catggccctc gatgcgcacg
     6181 atcagcggct cgaggatgcg tttctcgccg tgctgaaggg cgaggccgac aacgatggct
     6241 acaaccggct catcccgctc gcgggcgcgg gttggcagga ggcgggactg ttgcgcgcct
     6301 atggtgccta tctccggcag ctcgcggtgc ctttcgggca gcgatatctc gccgatgtga
     6361 tgaccttgca cccgggcatc gcccggcttc tcctcgatct cttccgcgcc cggctcgatc
     6421 ccgatctcgc catgacgggc gacgcgcggc ggagcgaggt ggcccgcatc gaggccgcac
     6481 tccagcaggc tctcggcaag gtcgcgagcc tcgacgagga ccggatcctc cgcctcttcc
     6541 tgtcagcgat taccgcgacg gtgcgcacaa acgtctatct tcgcgggccg gacggccgcg
     6601 ctccggaaac catcgcactc aagctcaagc cgcgcgagat cgaggccgcg ccccagccga
     6661 ggcctttcgc ggagatctgg gtgttctcgc cgcgcctcga tgcgctgcat ctgaggttcg
     6721 cgccgatcgc ccgcggcggc atccgctggt ccgatcgtcc gcaggatttc cgtaccgagg
     6781 tgctcggtct ctgcaaggcg cagcaggtca agaacgcggt gatcgtgccg gccggcgcca
     6841 agggcggatt cgtgccgaag ctcatcccgc gcaacgccac acgcgatgtg atccagaggg
     6901 agggcgtcgc ggtctacgag acgttcgtgc gcgccgcgct ctcgatcaca gacaatatcg
     6961 tcgacggcaa ggtgcttccg cccgagcgcg tcgtgcgcca cgacggcgac gacccctact
     7021 tcgtcgtggc cgccgacaag ggcaccgcaa ccttttccga catggcgaat gcgatctcgc
     7081 tcgagcgtgg cttctggctc ggcgatgctt tcgcgtcggg aggatcggca ggctacgacc
     7141 acaagaagat ggcgatcacc gcccgcggcg gctgggagtg cgtgaagcgg cacttccgcg
     7201 agatggatcg cgacatccag cgcgagccgt tcacggcggc gggtgtgggc gacatgtcag
     7261 gcgacgtgtt cggcaacgcc atgctgctct cgccagcaac gcgcctaatc gccgccttcg
     7321 accatcgcga catcttcgtc gatcccgacc cggatgcggc cgtatcgttt gccgagagga
     7381 agcgcctgtt cgatcttccg cgctcgagct gggcagacta taaccgcaag ctcatctcaa
     7441 agggcggcgg tgtctactcg cgggctgcca aatcgatcga gcttgcaccg gaagcgcgcc
     7501 agcggctcgg catcgcggcc accgccgtga cgcccgccga gctgatgcgc cacatcctgc
     7561 tcctcgacgt cgacctcgtc tggttcggcg gcatcggcac ctatgtgcgt gcgactagcg
     7621 aaaacgatct cgatgtcggc gaccgcggca acgatgcgat ccgcatcacg gggcgcgagc
     7681 tgcgcgcaaa agtcatcggc gaaggcgcga acctcgcgat gacgcagcgc ggccggatcg
     7741 agtatgcgct ccgcggcggg cggctcaata cggacttcat cgacaactcg gcgggcgtga
     7801 actcctccga ccaggaggtc aacatcaaga tcgcgctcgg acccgtcacg agatcggggc
     7861 gcctcgaccc cgaggcgcga aaggcattcc tcgcggggat gacggactcg gtcgcagcgg
     7921 cgtgcctgcg caacaactac cagcagtcgc tggcgctgag cctcgcggag cggaacagcg
     7981 ccgccgacat ggccttcaac gcacgcctca ttcgcgcgct cgcggctcgc ggtatgctcg
     8041 agcgccggct cgagtcctta ccgaccgacc tcgagctagg cgagcggcag aaggccggcc
     8101 aagggctcac ccggcccgag ctcgccatcc tgctcagcta ttcgaagatc gcgctttcgc
     8161 acgatctgct cgcgagcggg gcgcctgacg aggccatggt ctcgaacctc cttgaaggct
     8221 acccgccgcg cgccctcgcc aacgcgtatc acgctgagct gcgcgtgcac ccgcttcgcc
     8281 gcgaaatcat cgcgaccggc ctcacgaacc tcgtcgtaaa ccggtgcggt ccttcggcgc
     8341 cggtgcggct cgccgacgag accggccggt cgagtgtgga cgtggcgctc gcgatcttcg
     8401 cggcgcgctc gatcctggac ctgccgtcgc tctgggagcg gatcgacgcg ctcgatgcga
     8461 aagtcgccgg caatgcgcag ctcgatctct atgcgcgcgt ccaggaggca ctgatccgcg
     8521 cggcaacgac gctgctgcgg caaggcgatg cgagcacgct cggcgcccga atcgagacgc
     8581 atcacgccgc ggcgagcgag cttgccgggc tcgagcgcgg cgagctgccg acggggacgc
     8641 gcacgagcct cgagaaggac gtacgcgaaa tgacgacggt cggcctgccc gtggagcttg
     8701 ccgaacgcgt ggcggctctc cgcctgtcat cggaagcatc ggcggtcgcc gagctggccc
     8761 ggacggcggg ggcttcgatt gccgagaccg cgcgggttcg gctcgagatc agcgatcatc
     8821 tccagctcgg cgcgatcctc gacaaggcgc gcagcgttcc ggcgcccgac tactacgacc
     8881 ggctcgcggt cgcgtcggcg gaggcggcgc tcctcgacgc acaacgcatg atgacgatcg
     8941 cggcgatcga cgccgggcgc aaaccaggcc tcacgggtag cgcgttcgct gcatgggtcg
     9001 cctcgggcgg caagggcctc gaagggaccc gcggccggct cgccgagatc gcttccggat
     9061 cgggcttgtc ggtctcgcgg ctcaccgtcg ccgcggcgca ggcgcgcgag gccgccacgg
     9121 gctgagcgcg caacaaaagg cttgcggcgc ggccatcgct ggccgcgccg atggtctcag
     9181 gcgacgggct tcgtctcgcc gaacgccggc acgaccttgg cgaagccgtc gagccaggcg
     9241 acgagcttcg gatgggattt gcgccatttg ccctcgtgtc ggaagtcgag atagccgagg
     9301 gcggcggcga tcgtcacgtg gccgtaatcg ggcgagttgc cataggccgg cggcgccttc
     9361 tcgagctggc ctagggcgcg gtcgatcttc gcctgctggc ggtcgaccca ggcctgcacg
     9421 agcatggttt cggggcggaa gcgcttctcg tagacgagca ggagggccgc atcgaggatg
     9481 ccgtcgccga gcgcgcccat ggtgagcgtc ttccaacgct ccgtcccgga cttcgggaag
     9541 agcgccggcg cgggcttcag gctgtcgaga tactcacaga tcacgtggct gtcatagatc
     9601 gcgctgccgc cggcggggat gaggcagggg atcttgccga gcgggttctg ggcgttcagc
     9661 gtgttgtcgc ccttgttcgt gtccgcgggg accagctcga tgtcacccat cacgcccttc
     9721 atcagggcgg tcatcttgac tttgcgtccg tagggcgaaa gcggcgagga gagcagcttc
     9781 atctttgcca tggtggcctc cgatcgttga gcgacggcat ggtgccccgc gcggcgcgcg
     9841 ccggcaacgc tttccgcgcc gacacactcc ccggttcggc attgttggga tgcaattgag
     9901 cccatagact tcgagggacc cggagatctg ccgtcaacgt gagaaagggc gcgccatgac
     9961 cagcctcgca agcgtcagca aaggcacgcc gccgttcaaa acggacgaac tggaccgaca
    10021 gatggtggcg gcgggcctcg acatcatcgt cgcaacgtcc cgccacaacg tgcagtacct
    10081 gctcggcggc taccgcttct tcttcttcga gacgatggag gcgatgggca cgagccgcta
    10141 cctgccggcg gtgatctaca ggtgcggccg acccgcggag accgtgtatg tcggctgcgg
    10201 aatggagagc ttcgagcgcg acaacgggcg gctctggccg cagacgctgg acctcggcgc
    10261 gaccggcagc gaggcgacgg cccgccaggt tgtcggccac atcgagcggc tcggcggcga
    10321 gggcgccagt atcggcctcg aactgccgtt catcccggcc gatgccgctc acgtcattcg
    10381 tcaagggctc gcacgcagca ccgttctcga tgcgaccgta ccgctggaac ggctccgcgc
    10441 tgtcaagacg ccggccgaga tcggcatgct gcgccatgcc tcggagcagg tggtcgacgc
    10501 gatactggcg acgtttcgcg ccgtaaagcc ggggcagacc aagtccgaga tttcagacag
    10561 gctgaggcgc gaagaagtgc agcgcggtct cgtcttcgat tattgcctca tcacggcggg
    10621 aacgagcctc aaccgggcgc cgtcacccca ggcgatcaag gccggcgaca tcatctcgct
    10681 cgactccggc gcgaacaaca atggctacat cggcgatctt tgccgcatgg ggatactcgg
    10741 ccggccagac agcgaactcg tcgatctcct cgctcttgtc gaggaggtgc agcaggcggc
    10801 gcggcgtgtg gtacgggcgg gcacccgcgg cggcgaggta gtcgctgtcg gcgaggccat
    10861 ggtcaagcgc tcgccgcatg ccgcctacac gcactacatg gcgcatggca tgggcctcgt
    10921 cagccatgag gcgccacggc tcgcgacgat gccccgcttc tcctacagcg gcgacgatgc
    10981 cagcaaaccg ctcgccgccg gcatggtcct ttccatcgag acgaccatgg cccacccacg
    11041 ctgcgggttc atcaagctcg aggacacgat cctcgtgacg aaagacggcc atgaggcgct
    11101 aggcgatggc gcacgcggct ggaacgtcat tccactctga acagcgtatt cgcccgcatg
    11161 catgatcgtc gcagccgtgt tagactatag tctagcccga tgggtcagca tgcgatcaga
    11221 ggaggatgtg tgatgctgga ccagatcgaa catgcagcgt cggtctccat tgcacgcgcc
    11281 tgcggcttcg cgatgctcgg aatcgccacg ttcctcattg gtctctccgc cgtcgagacg
    11341 accttgatgc tcaagaccgg agggattctc acgctgatcg tctgcctcgc gctcgtcctc
    11401 aaggcaagcc ttgcggccgg acagccgtac aagagcaccg aggtctggat catgcttccg
    11461 ccggagcacc gcccgggcgc agccatcgcg cagcaggtga tcggcacggt cctgcgggaa
    11521 acgtatcttc gctttgccat ctatgcgtcg ctgctgtcgg cagctctctt tgctgcggct
    11581 ctcgtctccg gtctggcgca ataggtgcgg acgtctcgcg tcgtccgcag gccgaaaatg
    11641 acataaggat ttctttatat catcgttgcg ctcgtggtgt gtcggggcta taggaggccg
    11701 cgacgggcaa ggccggcgcg actgccgcgc gcttgccggc cagatatctg cgagcccgca
    11761 aagggacagg agcgcgacca atgagcaaga agaacgacnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccac atgaccatcc
    11941 agaccgcggt gctgatcgag acgctcgcgg ccctcggcgc ggacattcgc tgggcctcct
    12001 gcaacatcta ctcgacgcag gaccatgccg cctccgccat tgccgccgcc gggatcccgg
    12061 tcttcgccgt gaagggcgag agcctcgtgg agtattggga ctacacccgc aagatcctcg
    12121 aatggtccga cggcggcacg cccaacatga tcctcgacga cggcggcgac gcgaccatgc
    12181 tcgtccacta tggctt
//
LOCUS       DAORNH010000123        12156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3953_length_12156_cov_2.953888, whole genome shotgun sequence.
ACCESSION   DAORNH010000123 DAORNH010000000
VERSION     DAORNH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3953_length_12156_cov_2.953888"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1989)
                     /locus_tag="PK264_13180"
     CDS             complement(139..1989)
                     /locus_tag="PK264_13180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD76866.1"
                     /translation="MDADPQYRFLIGAHLDVATLAEASRAAIEAGTMTHEIILASGWV
                     GEQAYVDTLAAALGLERFETGVPGPSDRRLGDRVALVERDGAALLVVDAMARGPEQLA
                     RELDMSGWPPTRTALATRNEFDRLIATMNRKPWMAAAVEGLHEAQPHLSALHGPAAWQ
                     IAAGAMTFTIMAAGLFVAREATFALASVVVMAGFLSVAGLRLAALGQSWGQSKRHAAE
                     PRLHEADLPVYSLLVPLFREGAMVRELVGRLASLDYPSVKLDILLILEESDEETIAAI
                     GAIELPSSMRVVLVPKGGPQTKPKALNFALHLARGAYIVVYDAEDFPERDQLRRAVWA
                     FKSGDAELACVQACLNVYNPRASWLTRQFTLEYTAQFDAIFPALVSLGLPVPLGGTSN
                     HFPADVLRRLGAWDPYNVTEDADLGLRIARAGLRVEMLASTTWEEAPETFGPWLRQRT
                     RWLKGWFTTWLVHMRRPRSLLRDLGLRRFLGWQLLMAGIALSVLTHGLFSAVLLFEVV
                     FGGLRDGQTTWLGQVLGWAATVTMIVGLASAMVLGAVSARRRGRSYLARSLIWTPLYW
                     IAISLAAYRALWQLFRSPHHWEKTPHGIARHRDIPHRPPPGEAANRGPPA"
     gene            2453..3160
                     /locus_tag="PK264_13185"
     CDS             2453..3160
                     /locus_tag="PK264_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRD76867.1"
                     /translation="MRRVAIRFLTDNDFPPFNYLDEEGQLVGFNVDLARAICLELSVT
                     CDVQPKAWAELLPALGKGQADAVIAGHTPTAGTLAFADFTDAYMHTPARFVGRRGSGE
                     LRVAPDGLTGKTIGVAKGTAHEAYLRTFFHDSVIRTFENQELARDALVASQVDLIFDD
                     GISLVFWLNGTLSKECCELKGGPFFETRFFGDGMAIAVPKTDAQLKSLINRALRQIQA
                     SGRYDELILRYFPSRVY"
     gene            3168..3668
                     /locus_tag="PK264_13190"
     CDS             3168..3668
                     /locus_tag="PK264_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HRD76868.1"
                     /translation="MASDASRLRPEAYPAFSFAVDPAVAPFPTDRPLIVFDGVCVLCS
                     GFAKFVVRRDKRRRFLFTHAQSPLGAALFRHYGLDPLEFETNIVIIDGRAHGRLGAFA
                     AVMGELGWPWRAFTIANFLPTRLADWLYDRIAKNRYRLFGLTGVCMLPDASWRERLIG
                     DHATRD"
     gene            3701..4423
                     /locus_tag="PK264_13195"
     CDS             3701..4423
                     /locus_tag="PK264_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRD76869.1"
                     /translation="MREKDQVKAILFDVFGTVVDWRGGVIRELTAFGNGRGLKQDWAQ
                     FADDWRGLYQPSMEEVRAGRRPWVILDTLHRESLVKLLERHGIRGLSANDIEHLTTIW
                     HRLDPWPDVIAGLSRLRKRYIIGTLSNGNIGLLTRMAKRAGLPWDVVLCAEIARAYKP
                     MAECYVRNAEALNLRPEEVMLAAAHNGDLAAAAKAGLRTGFIVRPTEYGPNQRLDLKA
                     EQAWDVVTDSFLGLADAMGCPR"
     gene            4589..4828
                     /locus_tag="PK264_13200"
     CDS             4589..4828
                     /locus_tag="PK264_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76870.1"
                     /translation="MKAIAGLAVAGALLAMGVTTASAAPLARTEPAVTSSTPNIERVH
                     GWHRSCQWGPIRRWWHRHTSYGPQACRPYRRGYGY"
     gene            5112..5426
                     /locus_tag="PK264_13205"
     CDS             5112..5426
                     /locus_tag="PK264_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76871.1"
                     /translation="MKYSLTLALAGAGLAIMTSTAGAAPLPRTEAPAVVTSAVEPVHY
                     GSRGGYRRYYSGSYNYYWNTRPYYYNHYYYNPRPYYYNHYYYNPRPYFYFNYGARRHR
                     WY"
     gene            complement(5506..7632)
                     /locus_tag="PK264_13210"
     CDS             complement(5506..7632)
                     /locus_tag="PK264_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD76872.1"
                     /translation="MSSSSKSKSSGRSAGIAPPRAAKRPATQVHHGVTLRDDYGWLRD
                     PRWQDVMRDPSLLQPEIRTHIEAENAYTDAMLADTKALQEVLFQEMKGRIKEDDSSVP
                     APDGPFDYYSSYVTGGQYPRLCRRPRGGTGETILLDGNREAEGKPFWQIGATAHSPDH
                     KLLAYAVDDKGSELYVIRVRDLATGKDLADEIADTRGGIEWARDGRTFFYVRVDEMHR
                     PLIVCRHKIGTPASADVVVHEEKDPGFFAGVSQTHSGRYIMVGAHGTETDDFRLIDAE
                     RPESAPIVVAGRKSGHEYHVEHSGERLYILTNFADAEDYCIVETSTATPTPEHWRTLV
                     PHKPGRMIIEATAYRDHLVRLEREDGLPRIVIRRLKDGAEHAIAFEEEAYSLGISAGY
                     EFDTTTLRFTYSSMTSPTAVYDYDMETRARTLRKVQEVPSGHDPSDYITRRVLAPSHD
                     GELVPVSLLYRKSTPLDGSAPVFLYGYGAYGIAMPASFSTARLSLVDRGFIYALAHIR
                     GGRDRGTRWYRDGKLAKKTNTFRDFIAAGEHLVNLGLTRRGRIVANGGSAGGMLMGAV
                     ANMAPDLFLGIIADVPFVDVLNTMLDDTLPLTPPEFPEWGNPITDRAAFEAIRAYSPY
                     DNVAAKAYPHIFAYAGLTDPRVTYWEPAKWVARLRELKTDDNLLLLKTNMGAGHGGAS
                     GRFEQLVEIAVDYAFALKIAGLGKLS"
     gene            7955..8031
                     /locus_tag="PK264_13215"
     tRNA            7955..8031
                     /locus_tag="PK264_13215"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7989..7991,aa:Met,seq:cat)
     gene            complement(8286..8360)
                     /locus_tag="PK264_13220"
     tRNA            complement(8286..8360)
                     /locus_tag="PK264_13220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8326..8328),aa:Gly,seq:gcc)
     gene            complement(8578..9048)
                     /locus_tag="PK264_13225"
     CDS             complement(8578..9048)
                     /locus_tag="PK264_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRD76873.1"
                     /translation="MDAIDLKILAILQRDATLPVAEIGKEIGLSTTPCWRRIQRLEEQ
                     GVVQRRVAVLDPVAVNCGVTVFVSIKTDAHSLDWLEKFNSAVVDFPEVVEFYRMSGDV
                     DYLLRVVVPDIAAYDAFYKKLISRIEIAKVSSAFAMEQIKCTTELPLIFVAKRE"
     gene            9273..9770
                     /locus_tag="PK264_13230"
     CDS             9273..9770
                     /locus_tag="PK264_13230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HRD76874.1"
                     /translation="MADLKFITPTFAVTSALTEADFADLAARGVAAVISNRPDGEDGA
                     TLSARAEAVSAWRAGLKFRHLPVAKHEVFEEDAVHAMAEAIKSLDGVIVAHCKSGWRS
                     AILWAAASARTSPADCVAAAAKRAGFDLSEVREEIAAQAGKSRILSREAASALDCSES
                     VAAAA"
     gene            complement(9812..10513)
                     /locus_tag="PK264_13235"
     CDS             complement(9812..10513)
                     /locus_tag="PK264_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRD76875.1"
                     /translation="MSMQSPDQGGSRSREAARGANSWQARDRNAMAARLAADIPEGWS
                     VNLGIGIPLLVGDFVPAEREVIFHSENGIIGMGPLQKGPKLDTWIVNAGKQNVSLRTG
                     ASMVGHADSFAMIRGGHLDLCVLGAFEVAENGDFANWTLSLKSVPAVGGAMDLAAGAK
                     RLWLLMDHTSKDGKAKVKRSCELPLTAVKAVKRVYTDLAVLDVTPKGFVVLDIVAGLS
                     FEELQARTEAPLQRS"
     gene            complement(10537..11235)
                     /locus_tag="PK264_13240"
     CDS             complement(10537..11235)
                     /locus_tag="PK264_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRD76876.1"
                     /translation="MIDKRVASIAAAMAGVKDGSAVMIGGFGAVGHPRELVDGLIEQG
                     ARDLTVIANNAGSGRVEPLVRLLSAGRVRKIICSFPRSKGSVVFDELHKAGKLELELV
                     PQGTLAERIRAAGAGIPAFYTPTAFGTALAAGKETREIDGRGYVLEHALKADVALIEA
                     WQADRWGNLTYRTSARNFNPVMATAAGLVIAQSQHLVELGEIGPDMIVTPGVYVDRVV
                     HVPYGDPPPAIAND"
     gene            complement(11321..12046)
                     /locus_tag="PK264_13245"
     CDS             complement(11321..12046)
                     /locus_tag="PK264_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76877.1"
                     /translation="MTDHPLDGRVALVTGASRGIGKAVAKALAGAGAHVVLVARTVGG
                     LEETDDEIRALGGQATLVKLDLRNGEQLDALGPSLYQRWQRLDILVANAGILGPLTPV
                     PHVKTKDWAEVIEINLSANFRLIRTLDPLLRASDAGRAIFVSSGAALARNAYWGPYAV
                     SKSGLEALVKTYAQEMLATSVKANIVNPGPIRTGMRAKAFPGEDPETLKTADDLAPLF
                     LRLASPTCADTGRIFDFKTGDVR"
BASE COUNT         2080 a         3952 c         3988 g         2136 t
ORIGIN      
        1 cagctgccgc atctcttaca atcgcaacat cgagagcttc gcgccctcgt tccgccgggt
       61 cccggcgcgg cgcgctactg agacgtcgcg gtagccacgt tcgcgaagcg gcacgatccc
      121 gctcgagggg cggcgaactc aggccggcgg tccgcggttt gcggcctcgc ccggcggcgg
      181 ccggtgcggg atgtcacgat gccgggcaat gccgtggggc gtcttctccc aatggtgggg
      241 cgaccggaag agctgccaga gcgcccgata ggccgcgagc gaaatcgcga tccagtagag
      301 gggtgtccag atgagggatc gcgcaagata gctgcggccg cgccgccggg cggagaccgc
      361 accgagcacc atcgccgacg cgagaccgac gatcatggtg acggttgcag cccacccgag
      421 gacctggccg agccatgtgg tctgtccgtc gcggagcccg ccgaagacga cctcgaaaag
      481 caggacggca ctgaacaggc cgtgcgtgag caccgacaag gcgatcccgg ccatgaggag
      541 ctgccagccg aggaagcgcc gcaagcctag gtcgcggagt agcgaccggg gccgacgcat
      601 gtgcacgagc catgtcgtga accagccctt gagccagcgc gtccgctgcc tgagccacgg
      661 cccgaacgtc tctggcgcct cctcccacgt cgtcgacgcc agcatctcga cgcggagccc
      721 agcacgcgcg atcctgaggc cgaggtcggc gtcctcggtg acattgtagg gatcccatgc
      781 gccgagccgc ctcaggacgt ctgccggaaa gtggttggag gtgccgccga gcggcaccgg
      841 cagcccgagg ctaacgagcg ccgggaagat cgcgtcgaac tgagcggtgt actcgagcgt
      901 gaattgtcgc gtgagccagc tcgcccgcgg attgtagacg ttgaggcagg cttgcacaca
      961 ggcgagttca gcgtccccgc tcttgaaggc ccagacggcg cgccgcagct ggtcgcgttc
     1021 gggaaagtcc tcggcgtcgt agacaacgat ataggcgcca cgcgccagat ggagggcaaa
     1081 attgagcgcc ttcggcttcg tctgcgggcc gcccttcggc accaggacga cacgcatcga
     1141 cgacggcagc tcgatggcac cgattgccgc aatcgtctcc tcgtcgctct cctcgagaat
     1201 gaggaggata tcgagcttca cgctcggata gtcgagcgag gcaaggcgcc cgacgagctc
     1261 gcgcaccatc gcgccttcgc ggaagagcgg gacaagcagc gaatagaccg gcaagtcagc
     1321 ctcgtgcagc cgcggctcgg ccgcgtggcg tttcgattgc ccccacgact gtccgagtgc
     1381 cgcaagccgc agtccggcga ctgagaggaa gcccgccatc acgacgaccg aggcgagcgc
     1441 gaatgttgcc tctcgtgcca cgaagagtcc tgccgccatg attgtgaaag tcatagcgcc
     1501 ggccgcgatc tgccaggccg ccgggccatg aagggcgctg aggtgtggct gcgcctcgtg
     1561 caagccctcg accgcggccg ccatccaagg cttgcggttc atcgttgcga tcagccgatc
     1621 gaactcgttc cgcgtcgcca aggctgtccg cgtcggcggc cagccgctca tgtcgagttc
     1681 tcgcgcaagc tgctcgggtc cgcgcgccat cgcatcgacc accagaagtg ccgcgccgtc
     1741 gcgctcgacg agtgccaccc tgtcgccgag cctgcggtcg ctggggcccg gcacaccagt
     1801 ctcgaagcgc tcgagcccga gcgctgcggc gagcgtgtcg acgtaggctt gctcgccgac
     1861 ccagccactc gcgagtatga tctcgtgcgt catggtgccg gcctcgatcg ctgcacgcga
     1921 ggcctctgcc agcgttgcga catcgagatg tgcgccgatc aggaagcgat actgcgggtc
     1981 ggcgtccacc ggaaacgcat gctccaggcg ctcggagatg cgacgcaatc gagctagact
     2041 gtcgtgatga gtcgtcgcat cacgttcaat aggtcgcggg gctcgtcgat gctggtttgt
     2101 cagcgccgcc ccctacgcgt gcgtgttgcc tgcgccggtc gggatcggac attgctctgc
     2161 tggggcggac tgaatcttgg aactccttcg gtcgaaactc tgctcgctca caaattcgct
     2221 ccgagaggct cgccttgcgc gtgccgcgat cgtgtcggac tggaaaagat catctgaggc
     2281 aggcctatcg actatgccat atcacaatca gggttcaaga cctcgcggcg actcagcccc
     2341 gggcacatcg ttcgtgtggt gccttgccct cctgatcgcg ctagccgcag tctgtttcgc
     2401 cccgcccgcc accgggcagg gccgcacgcc gccccgcgcc gacgacgatg tcgtgaggcg
     2461 cgttgcgatc cgttttctca ccgacaacga ctttcctccg ttcaactatc tcgacgagga
     2521 aggacagttg gtcggcttca atgtcgatct cgcccgcgcc atctgcctcg agctgtcggt
     2581 gacgtgcgat gtccagccga aggcctgggc cgagctcctg cccgcgctcg gcaagggcca
     2641 ggcggacgcg gtgatcgctg ggcacacgcc gacggcaggc acgctcgcct tcgcagactt
     2701 cactgatgct tacatgcata cgcccgcgcg cttcgtcggc cggcgtggct ctggcgagct
     2761 gcgcgtcgcc cccgacgggc tcaccggcaa gacgatcggc gtcgccaagg gcaccgcgca
     2821 cgaggcctac ctgcgcacct tcttccacga cagcgtgatc aggacgttcg agaaccagga
     2881 gcttgcccgt gacgcgctgg tggcaagcca ggtcgatctc atcttcgacg acggcatcag
     2941 cctcgtcttc tggctgaatg gcacgctctc gaaggagtgc tgcgagctga agggcggccc
     3001 cttcttcgag acacgattct tcggcgatgg catggcgatc gccgtgccga agacggatgc
     3061 acaattgaag agcctcatca accgcgcgct gcgccagatc caggcaagcg gccgctacga
     3121 cgagctgatc ctcaggtatt ttcccagtcg cgtctattga gctcggcatg gcaagcgacg
     3181 catcaagact gcggccggag gcgtacccag cattcagctt cgccgtcgat ccggccgtgg
     3241 ccccattccc gacggaccgc ccgctcatcg tcttcgacgg ggtgtgcgtt ctctgctcgg
     3301 gctttgcgaa attcgtcgta cgtcgcgaca agcgtcgccg gtttctgttc acccacgccc
     3361 agtcgccgct cggcgccgcg ctctttcgcc actacggcct cgacccgctc gagttcgaga
     3421 ccaatatcgt catcatcgat gggcgcgcgc atggccggct cggcgccttc gccgcggtca
     3481 tgggcgagct cggctggccg tggcgggcat tcactatcgc caattttctg ccgacacggc
     3541 tcgccgattg gctctacgat cggatcgcca agaaccgcta tcgtctgttt ggactcaccg
     3601 gggtttgcat gctccccgat gcgagctggc gcgagcgatt gatcggcgat cacgcgaccc
     3661 gggattaggc tagatcggca tctgcaggag cgacatcgac atgcgtgaga aggaccaagt
     3721 caaagccatc ctgttcgacg ttttcggaac ggtcgtcgac tggcgcggcg gcgtcattcg
     3781 cgagctcacg gccttcggca acggccgtgg cttgaagcag gactgggccc agttcgccga
     3841 tgactggcgc ggcctctacc agccgtcaat ggaagaggtc cgcgcaggac gccgcccctg
     3901 ggtgatcctc gatacgctgc atcgcgaaag cctcgtgaag ctcctcgagc ggcacggcat
     3961 ccgcggcctc agcgccaacg acatcgagca cctgacgacc atctggcatc ggctcgaccc
     4021 ctggcccgat gtcatcgcgg ggctctcccg gctcaggaag cgttacatca ttggtacatt
     4081 gtccaacggc aatatcgggc ttctgacgcg catggcgaag cgcgccgggt tgccatggga
     4141 cgtggtactc tgcgccgaga tcgcacgtgc ctacaagccg atggccgagt gctacgtgcg
     4201 caatgccgag gcgctgaacc tcaggccaga ggaggtgatg ctcgccgcag cccacaacgg
     4261 cgatctcgcc gccgcggcga aggcgggtct gaggaccggc ttcatcgtgc gcccgaccga
     4321 gtacgggccg aaccagcgtc tcgatctcaa ggccgagcag gcctgggatg tcgtcacaga
     4381 cagcttcctc gggctggccg acgccatggg atgcccgcga taagtcgtcg gagcgctgct
     4441 gatccaggct gacaaagcca tacctattca ggtagcgttc agatgggcac gccaaaactg
     4501 gcgccatact ggattgcgaa gtccggtcaa ggggcgatcg gtggccccgg tatcccccga
     4561 acaccgaagc gaaaatggag cactgatcat gaaggctatc gcaggtctcg cagttgccgg
     4621 cgcgctgttg gccatgggcg tgacgaccgc atcggcagcg ccgctcgcgc ggacagagcc
     4681 ggccgtcacc agctcgaccc cgaacatcga gcgcgtacat ggttggcacc gcagctgcca
     4741 gtggggcccg atccgccgct ggtggcaccg ccacacgagc tacggtcctc aggcctgccg
     4801 tccgtatcgc cgcggctacg gctactgagg tcggtcgacg tctcgatcgg tcgtaggccc
     4861 cgtgcgctgc gcggggcctt ttcacaacca cgatttcaca tccacgtttt catgtctcga
     4921 tcggtcgtgc acaacgcctt tcgtgtatcg caatgtttcc atcaatcgga cattcatccg
     4981 tcattcatgt ggccggcgtc acgcttcggc catgctcgat cggggagtcc ggcgagcgag
     5041 actaagtaat gccgaacgtg gcggttcggg atgccgggaa cctgtcgaac cagttctttg
     5101 gagcaatccc catgaagtac tcactgacac tcgcgctggc cggtgccggc ctcgccatca
     5161 tgacatcgac tgcaggtgcg gcccccctgc ctcgcaccga ggctccggcc gtcgtcacga
     5221 gcgccgttga gcctgtgcac tatggctcga gaggcggcta tcgccgatat tactcgggct
     5281 cgtataacta ctattggaat acgcggccct actactacaa tcactactat tacaacccac
     5341 ggccgtacta ctacaatcac tactactaca acccgcggcc ctacttctat ttcaactacg
     5401 gtgcccggcg ccatcgctgg tactgagttg tcatagccga gcgccatcta tcgaggcgcg
     5461 ttcccccgcg gcatgccgcg ggggatttgc ttttcgaggc cggcctcaag agagcttgcc
     5521 gagccccgcg atcttcaagg cgaacgcgta gtcgacggcg atctcgacga gctgctcgaa
     5581 gcggcccgac gcgccgccat gccccgcgcc catgttggtc ttgaggagca gcaggttgtc
     5641 gtcggtcttc agttcgcgaa gccgcgccac ccacttcgcc ggctcccagt aggtgacgcg
     5701 cggatcggtg aggcctgcgt atgcaaagat gtgcgggtag gccttcgccg cgacgttgtc
     5761 atagggactg taggcgcgga tcgcctcgaa ggctgcccgg tccgtgatcg ggttgcccca
     5821 ctccgggaac tcgggcggcg tcaacggcag cgtgtcgtcg agcatcgtgt tcagaacatc
     5881 gacgaacggc acgtcggcaa tgatgccgag aaagagatcc ggtgccatgt tcgcgacagc
     5941 gcccatcagc atgccgccgg ccgagccacc gttggcgacg atccgcccac gtcgtgtgag
     6001 gccgaggttc acgaggtgct cacccgccgc gatgaaatcg cggaacgtgt tcgtcttctt
     6061 cgcgagcttc ccgtcgcgat accagcgcgt gccgcgatcg cggccgccgc gaatgtgcgc
     6121 cagcgcatag atgaagcccc gatcgacgag cgagaggcgc gccgtcgaga acgaggccgg
     6181 catggcgatg ccgtaggcgc cgtagccgta gagaaacaca ggcgccgagc cgtcgagcgg
     6241 cgtcgacttg cgatagagca gcgagaccgg aaccagttcg ccgtcgtgcg agggcgccag
     6301 aacgcgccgc gtgatgtagt cactcggatc gtgcccgctc ggcacttcct ggaccttgcg
     6361 caacgtgcgc gcgcgcgtct ccatgtcgta gtcgtagacc gcggtcggcg acgtcatcga
     6421 cgagtatgtg aatcgcagcg tcgtcgtgtc gaactcgtag ccggcgctga taccgagcga
     6481 ataggcctcc tcctcgaagg cgatggcgtg ctccgcgcca tccttgagcc ggcgaatgac
     6541 gatgcgcggg aggccgtcct cgcgttcgag gcgcacgagg tggtcgcggt acgccgtcgc
     6601 ctcgatgatc atgcggccgg gcttgtgcgg cacgagcgtg cgccagtgct cgggcgtcgg
     6661 cgtcgccgtc gaggtctcga cgatgcaata gtcctcggcg tcggcgaagt tcgtgaggat
     6721 gtagagccgc tcacccgaat gctcgacatg atactcgtgg ccgctcttcc tgccggcgac
     6781 gacgatcggc gcgctctccg gccgttctgc atcgatcagg cgaaagtcgt cggtctcggt
     6841 gccgtgagcg ccgaccatga tgtagcggcc gctgtgcgtc tggctgacgc cagcgaagaa
     6901 gcccgggtcc ttctcctcgt gaaccaccac atccgcgctc gccggcgtgc cgatcttgtg
     6961 gcggcagacg atcaacgggc ggtgcatctc gtccacgcgg acgtagaaga acgtgcgccc
     7021 gtccctcgcc cattcgatgc cgccgcgcgt atccgcgatc tcgtcggcga ggtccttccc
     7081 cgtggcaaga tcgcgaacgc ggatgacata gagctccgat cccttgtcgt cgaccgcgta
     7141 ggccagcagt ttgtggtcgg ggctatgggc ggttgcgccg atctgccaga agggcttgcc
     7201 ctcggcctcg cggttgccgt cgagcaggat cgtctcaccg gtgccgccac gcgggcgccg
     7261 gcacaggcgc ggatactgcc ctccggtcac atagctcgaa tagtagtcga acgggccatc
     7321 gggcgccgga accgagctat cgtcctcctt gatgcgcccc ttcatctcct ggaacagcac
     7381 ctcctgcagc gccttcgtat cggcaagcat ggcgtcggta tatgcgttct cggcctcgat
     7441 gtgtgtgcgg atctcgggct gcagcaatga cggatcgcgc atcacgtcct gccagcgcgg
     7501 atcgcggagc cagccgtagt cgtcgcgcaa tgtgacgccg tgatggacct gcgtcgccgg
     7561 gcgcttggcg gcgcgcggtg gagcaatgcc cgccgaacgg ccggaagact tcgatttgga
     7621 actcgatgac atggggaccc tcctcgtcga ggaacaaaag cagtctgatt cgggcgcggg
     7681 gatggctcgc ggacggcgcc aattcggcgg gtaaaggcac gagcgagcta caggctcctc
     7741 gggtgtcgag ctaaggttgc cgctacgctt agcatcgcgg cgacgccctc gccattgtgc
     7801 tcgccgaaag cagtgggcag gctgaagatg gagccagcct gaaccattca ccacggcgcg
     7861 gaacgcgatc gctcagggcg ggaccgatcc ggcaggagga tgcgggacac gaccttgaca
     7921 cttcggccgt ctcttcccta taagccgcga gcgcggcgga gtagctcagc tggttagagc
     7981 gacggaatca taatccgcag gtcgggggtt caagtccctc ctccgctacc aaacgtcatc
     8041 tcgatggctc attggacagg caattctcgc cgtcagcgcg attgccatcg gtgggcgcag
     8101 aatggtgggg cgccgccaag cgagatgatg ctggccggcc cgctcaattt tctgggcacg
     8161 tacaatcgtt gcgtttggat cggacgcact cgggctgcca gcaatgaggg tgcgcgtacg
     8221 cctttgccag ccgcaacttt cacgccaagc tgcagatggc gctagccgaa cgtctatgaa
     8281 caaattggag cgggcgaagg gatttgaacc ctcgaccctc acgttggcaa cgtgatgctc
     8341 tacccctgag ctacacccgc ttccgatgtg tcatgccgca ttggcgacag ggggctatat
     8401 ggcgcaaagc gatctgaatt gcaagcgacg tcaaatgggc tcagcgctca gtggcagggt
     8461 gtttaagctc tccacttctg gtgtccggcc gagcagagca agagcagggc gccatacccg
     8521 ttcaagggtc aaggacctct gccctcagct tgttcgtact cagcggccgg ggctccgtca
     8581 ctcgcgtttg gcgacgaaga tcaggggcag ctcggtcgtg cacttgattt gctccatggc
     8641 gaaggccgag gagaccttgg cgatctcgat gcgcgagatg agcttcttgt agaaggcgtc
     8701 gtaggcagcg atgtcgggta ctacgacgcg cagcagatag tcgacgtcgc cggacatgcg
     8761 ataaaattcc acgacctcgg ggaagtccac gacggctgag ttgaattttt cgagccagtc
     8821 gaggctgtga gcgtcggtct tgatggagac gaagaccgtg acgccgcagt tgaccgcgac
     8881 cgggtcgagc acggcgacgc ggcgctggac cacgccctgc tcctcgagcc gctgaatgcg
     8941 ccgccaacag ggcgtcgtcg agaggccgat ctccttgccg atctcggcga cgggaagggt
     9001 cgcgtcgcgc tgcaggatgg cgaggatctt gaggtcgatt gcgtccagca tgggggcctt
     9061 ggaacgtcgt tctcttcaaa gtcgataata gcaaaaaaat ccccattgtc tacggttcga
     9121 ggctgatatt tggtattttt ttcttcagcc ctcttgagtg tgtcctccga tcccctcata
     9181 ctgtgctgca agacacgctc tcgtcggagc atgggtgtta gcaagtgcga aacatcgcct
     9241 aacacctgag ccgtccatca gaagtgagtg ccatggccga cctgaagttc atcaccccga
     9301 cctttgctgt gacctcggcg ctgaccgagg ccgacttcgc tgatctcgcg gcgcggggcg
     9361 ttgcggccgt gatctccaac cgaccggatg gcgaggacgg ggcaacgctg tcggcgcgcg
     9421 ccgaagccgt ctccgcatgg cgcgcgggtc tcaagttccg ccatctgccc gttgccaagc
     9481 atgaggtctt cgaggaggac gcggtgcacg ccatggccga ggcgatcaag agcctcgatg
     9541 gcgtgatcgt cgctcattgc aagtcgggct ggcgctcggc gatcctttgg gccgctgcga
     9601 gtgcccgcac atcgcccgcc gattgcgttg ccgcagcggc gaagcgcgcc ggcttcgacc
     9661 tctccgaggt gcgcgaggag atcgcggccc aggccggcaa gagccgcatc ctctcgcgcg
     9721 aggctgcctc ggccctcgac tgcagcgaaa gcgtcgccgc agccgcgtga gcgcgtgcag
     9781 cccggccgct aacgctgcga tttcagtggt ctcaggatcg ctgcaggggc gcctcggtgc
     9841 gggcctgcag ctcctcgaac gatagcccgg cgacgatatc gagcacgacg aaacccttcg
     9901 gcgtcacgtc gagcacggcg aggtccgtgt agacgcgctt caccgccttg accgccgtca
     9961 gcggaagctc acagctgcgc ttcaccttcg ccttgccgtc cttcgaggtg tggtccatca
    10021 gcagccagag ccgtttggca ccggcagcga ggtccattgc gccaccaacc gcggggacag
    10081 acttgagcga gagcgtccag ttggcaaaat cgccattctc cgcgacctcg aaggcgccga
    10141 gcacgcagag atcgagatgc cccccgcgga tcatggcgaa gctgtcggcg tggccgacca
    10201 tcgacgcgcc cgtgcgcagc gacacgttct gcttgccggc gttgacgatc caggtatcga
    10261 gcttcggccc cttctgcagc ggccccatgc cgatgatgcc gttctccgaa tggaagatca
    10321 cctcgcgctc cgccggcacg aaatcgccga ccagcagggg gatgccgatg ccgagattca
    10381 ctgaccagcc ctcaggaatg tccgcagcga ggcgcgccgc catggcgttg cgatcgcggg
    10441 cctgccagct gttggcgccg cgcgcggctt cacggctgcg gctgccgcct tgatcaggcg
    10501 attgcatcga catggaggct gcctcctggt tgagggtcaa tcgttggcga tggccggcgg
    10561 cgggtcgcca tatggaacat gcacgacacg atcgacgtag acgccgggcg tcacgatcat
    10621 atcggggccg atctcgccga gctcgacgag gtgctggctc tgggcgatga caaggccggc
    10681 ggcggtcgcc atgacgggat tgaagttgcg cgccgaggtc cggtacgtga ggttgcccca
    10741 gcgatctgcc tgccaggcct cgatcaaggc gacgtcggcc ttgagcgcgt gttcgagcac
    10801 gtaaccgcgt ccgtcgatct cgcgcgtttc cttgccggcg gcgagcgcgg taccgaatgc
    10861 agtcggggtg tagaaggccg ggatgccggc gccggccgcc ctgatgcgct ccgcgagcgt
    10921 gccctgagga acgagctcca gctcgagctt gccggccttg tgcagctcat cgaacacgac
    10981 ggagcccttc gagcgcggaa agctgcagat gatcttgcgc acgcgccctg ccgataggag
    11041 gcgcacgagc ggctcgacgc ggccggagcc cgcattgttg gcgatgaccg tgaggtcgcg
    11101 cgcgccctgt tcgatcaggc cgtcgacgag ttcgcgcgga tggccgacgg cgccgaaacc
    11161 gccgatcatc actgcggagc catccttcac tccggccatc gcggcggcga tgctcgccac
    11221 cctcttgtcg atcatgtctc actccgcctc gcccgatcgc atcggggcgc cgttcgtcgg
    11281 gcgtgatctt ggcgcaatcg tccggcggcg acaaatgcga ttagcgcacg tcgccggtct
    11341 tgaaatcgaa gatccggccc gtatccgcgc aggtcggact ggcgaggcgg aggaacagag
    11401 gcgcgaggtc atcggcagtc ttcagcgtct cagggtcctc cccggggaac gccttggcgc
    11461 gcatgccggt gcggatcggc cccggattca caatgttcgc cttgaccgag gtcgcgagca
    11521 tctcctgggc gtaggtcttg accagggcct cgaggccgga cttcgaaacg gcgtatggcc
    11581 cccagtaggc attgcgggcc agcgccgccc cggaagagac gaagatcgcg cggccggcgt
    11641 ccgaggcgcg cagcagcgga tcgagagtgc ggatcagtcg gaaattggcg ctgaggttga
    11701 tctcgatgac ttccgcccag tcctttgtct tgacatgcgg caccggcgtc agcggcccga
    11761 ggatgccggc gttggcgaca aggatgtcga ggcgctgcca acgctgatag agcgatggcc
    11821 cgagcgcgtc gagctgctcg ccgttgcgca ggtcgagctt gacgagcgtc gcctggccgc
    11881 caagggcgcg aatctcgtcg tcggtctcct cgagaccgcc gaccgtgcgg gccaccagga
    11941 cgacatgggc gcccgcgccg gcgagcgctt tggcgacagc cttgccgatg ccacgcgatg
    12001 cgcccgtgac gagtgctacg cggccgtcca gtgggtgatc agtcatcgtg cctctctgcg
    12061 ggccgaggag tggggcaagc gagacatcag ccgcctcttt tcttgggcgc ggcgggcggc
    12121 gccggagcgg caggtgcgac aggggcggga ggcgcg
//
LOCUS       DAORNH010000124        12136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_3963_length_12136_cov_2.723036, whole genome shotgun sequence.
ACCESSION   DAORNH010000124 DAORNH010000000
VERSION     DAORNH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3963_length_12136_cov_2.723036"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PK264_13250"
     CDS             <1..494
                     /locus_tag="PK264_13250"
                     /inference="COORDINATES: protein motif:HMM:NF025135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HRD76878.1"
                     /translation="PPLRRLHAFEGACRYSGTASVEGAGNSVAWAIARLFGFPKATPR
                     IEVEVQFTRVNGVETWRRRFAGAAFASTQEIGRGRNEGLLIERFGPLAFGLAVTEQGG
                     RLGLEVRRWTLLGVPMPRRLAPWTRASEHDAGDRFNFDVEIGLPIVGRLVRYRGHLEP
                     VAD"
     gene            807..1070
                     /locus_tag="PK264_13255"
     CDS             807..1070
                     /locus_tag="PK264_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76879.1"
                     /translation="MRINPKAAADPPPPSAPAKPKVVKAAPPAVDATVKAPPAKTAAA
                     SPSGFITRARCVQMIRNKYKFIYAATDWQTKIDLCVTTKGQTF"
     gene            complement(1172..1453)
                     /locus_tag="PK264_13260"
     CDS             complement(1172..1453)
                     /locus_tag="PK264_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HRD76880.1"
                     /translation="MTEQQFRPRTRYVIGPDGSPLTIADLPPKDTKRWVIRRKAEVVA
                     AVRGGLLSLEEACERYKLTVDEFLSWQRSIDKHGLPGLRATRLQDYRGG"
     gene            complement(1781..2467)
                     /locus_tag="PK264_13265"
     CDS             complement(1781..2467)
                     /locus_tag="PK264_13265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRD76881.1"
                     /translation="MFVPIHDENPLRHIRLAWVTWAIIAINVVVLALQVTGNRATYAA
                     SFAVVPTELLGVRIFGGPANGPNDALAVPEGLTLLTYMFLHGDPLHLVGNMLFLWVFG
                     DNVEDALGHLKFAVFYLLCGIAGGLAHAYVVPTSNLPLVGASGAVAGVIGAYLLLHPK
                     VRLWVLVMRVVPLNISAAWALGAWILTQLVMVLLPQVGPTAWWAHIGGLAAGAALVLV
                     LRRPGVPILD"
     gene            complement(2505..>3071)
                     /locus_tag="PK264_13270"
     CDS             complement(2505..>3071)
                     /locus_tag="PK264_13270"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215690.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRD76882.1"
                     /translation="PDSGVPAAIGFAEFAHIPFELGIIRNHYVGRTFIEPEQRIRAFG
                     VKLKHSANKGVIKGARVVLIDDSVVRGTTSMKIVALLKDAGAREVHMRIASPPITHPD
                     FYGIDTPSKQELLAANKSIEEMRRYMGADSLAFISVAGIYRAMGQDRRDNRNPQFTDH
                     CFTGDYPTPLADAEAPDIRQLSLLTETI"
     assembly_gap    3072..3081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3082..4044)
                     /locus_tag="PK264_13275"
     CDS             complement(<3082..4044)
                     /locus_tag="PK264_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRD76883.1"
                     /translation="MAVRAASTRLSAQALPPAPQPNALPPAPTFNRHGDDRLREECGV
                     FGIFGHDDAAALTVLGLHALQHRGQEGCGIVTYDGTQFKQERRLGLVGDNFNKADVIA
                     KLKGHIAIGHNRYSTTGDVVLRNVQPFFADIDGTGLAIGHNGNLTNARTLRQELSSEG
                     ALFQATSDTEVILHLTARSRERAITDRLIEALRQIEGAYALTCMTSDMLIGVRDPVGI
                     RPLVIGRIGKAYVLASETCALDIVGAEYERDVENGEMVVITSAGMRSIRAFPKRQARP
                     CIFEYIYFARPDSIVGGRSVYGIRKLMGAELARESGVSADVVVPV"
     gene            complement(4250..5245)
                     /locus_tag="PK264_13280"
     CDS             complement(4250..5245)
                     /locus_tag="PK264_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HRD76884.1"
                     /translation="MTGLVTRAWSEVWPISGQFVISRGARTEARVVVAEAEIDGVTGR
                     GECTPYPRYGETIEGTLSAIEALDGRLYGPPKVARARLASILPPSAARNAVDCAIWDL
                     EARLSGQPVHILAGLPAPRPVLTCYTLSLGTPEAMADAARRHADKPLLKLKLGGAGDA
                     ERMRAVREARPDARLVADANEAWQAADLEPLLAAAADAGLELIEQPLPQGADDALARI
                     KRIVPVCADESAHTTDSLDALRGRYDAVNIKLDKTGGLTEAIRMHAEAHRLGFRIMVG
                     CMLATSLSMAPAALIATEGDFADIDGPLLLARDRAPGISYANGQASLPATGLWGS"
     gene            complement(5214..6887)
                     /locus_tag="PK264_13285"
     CDS             complement(5214..6887)
                     /locus_tag="PK264_13285"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD76885.1"
                     /translation="MVRAHSDRADAQLSFDVVIVGAGFAGLYAIHKLRGMGLSVRAIE
                     ANAGLGGTWYANRYPGCRCDFESLDYSYSFSEELQQSWNWPERFSSRADIHRYVEHVA
                     DRFDLRRDIELSTRVSSARFDRSAARWLITTDRGTRHHARFLVMATGNLSVPLIPEVL
                     DPVRFKGPAHHTAGWPDEGVDVSGKVVGVIGTGASAIQAIPELAREARHLYVFQRTPN
                     FVLPSRNAPADPEETRRHKANYRTKREQARLSPFGLSGYVNPTHCAMDVTPEVRKARF
                     EAAWQAGDTVAFQFAFTDILINKGANNAAAEFAREKIREVVRDPAVAERLTPRGYPIG
                     ARRLLIGSNYYETFNRQNVTLVDVESDPIVGIEAEGVQTSDRLYPLDVLCLATGFDAV
                     TGALDRIAIEGGDGVSLKAAWADGPRTFLGLMTARFPNLFMLMGPQSPGVRSQMILSA
                     EHHVELVATLLAEIRRRNATIIEADPTAENDWVTHSRSVADKTLYPLAKSWYLRPDPS
                     GKRSTVLPYVGGVHVYRRLCQQMIDRGFQGFRISGREALHDRVGHKSMV"
     gene            complement(7083..8411)
                     /locus_tag="PK264_13290"
     CDS             complement(7083..8411)
                     /locus_tag="PK264_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD76886.1"
                     /translation="MAPLDMKPNELEPYWLPFTPNRSFKKKPRLFSGAKDMHYLTPDG
                     RKVIDATSGLFCVNAGHGRKTIVEAIQKTAAELDFSPPFQYAHPKVFELSSKLTLLAP
                     GDLDYCLFANSGSEAVDTALKVALAYHRSRGEGSRTRFIGRERGYHGVGFGGISVGGM
                     VANRKMFGNMLAGVDHLPTTYNRAKQAFSNQEPEWGSETADELTRLIGLHDASTIAAV
                     IVEPVAGSTGCIPPPKGYLEKLRAITEKHGILLIFDEVITGFGRLGSCFAAERYNIVP
                     DMITFAKGVTNAAVPMSGVLVRKGIYEAHMSGPEHMVELFHGYTYSGHPLAVAAGLAT
                     LDIYKNEGLFERAKSLEKTFADAMLSLKGLPHVADIRPIGLMCGIDLDPVDGKVGLRG
                     YNAIESMYHDHDLYVRVTADTLIVAPPLIVSEAQIGEIRDRIATVLKAVA"
     gene            complement(8563..11205)
                     /locus_tag="PK264_13295"
     CDS             complement(8563..11205)
                     /locus_tag="PK264_13295"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRD76887.1"
                     /translation="MNKPPIDLMRALEEFSGVGDPGALEAMFDGRPGAGPRPAQRSSA
                     SAAAPGLTTLLEAYRHRGFLRARLDPLGLAPRPEIPELDPAAHGLARRDAAELAERLE
                     RAYCGSIGWEIGHIHDGARRRWLEAEAEQADPVTPDATERRRILALLARVDAFESGLN
                     LRIPAGKHFGIGGAETFLVALETLIAESARLGVEEVVVGGMHRGRFAMLATIMEKPLT
                     ALIAEIQGKPAIPEGMKASSDVSYHLGYSGDRQIAGSTVHFSVSPHPSHLQLAPIITQ
                     GRARAKQTLLGPDGARRVLPLLLHTDASFSGQGLVAEMLQLSGLAPFRLGGTVHVLIN
                     NQLGFTTTPEEGRTARGASDIARLIEAPVLHVNGDDPEAVWRVALIAANWRARFQSDI
                     IIDLVCYRRPGHNEIDEPRFTQPRMYEAIDARPPVRALYADELAARGLDTDAADSATD
                     AMRASLGSAFAAAKSWKINQADWFEGRWTGLVQGDEAAMLAPVVTGVPAARLTDIGRA
                     ITSLPDGTTLDPKVARFLDERRRSIEAGEGINWATGEALALASLLSDGTSVRLGGQDS
                     VRGAFTQRHLEVHDQASGERHLTLAPAARAFAKAEIHNTPLIEHAVLTYEYGISLADP
                     ARLVIWEAQFGEFLNYAQPVFDQCIACGEDRWLRSSGLVILLPHGLDGGGPDHSTGRP
                     ERLLAACAGANLQVVNASTPANFFHALRRQMARPFRKPLVVLTPKALLRHKACVSTLA
                     DFAEGTGFRHVIDDTGVAGASRVLLCTGKIFYDLAAERAARGLGQKVAIVRIEQLHPL
                     PADEIRTAMTRHSGAETIWCEEEPENMGYFTHLDRPLEAITGRRVRRAGRPAVATPAV
                     GVKYWHEAEIKAVLDTAFADLTSA"
     gene            complement(11223..12122)
                     /locus_tag="PK264_13300"
     CDS             complement(11223..12122)
                     /locus_tag="PK264_13300"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76888.1"
                     /translation="MGFVGVGKMGGPMVKRLLAAGYDVVVSDVDEKAIADVVAKGARD
                     GGTPEQVASTVETIFVSLPTPPIVKAVMLGPGGIAAGKKRRVVVDLSTTGPSMAKEIQ
                     AELDKKGVIQIDAPVSGGVTGAEKGTLACMVSGPKAECDKLEPVFGVFGKYFYIGPKP
                     GTGQMMKLANNLLSATNMVAAMEVAIMGVKFGLDANVMIDVINASTGRNTATEDKFPR
                     RILPRTFDHGFSMGLMTKDVNLAIEEAEKLGIPMFVGNAVKGMWNLGLNKGGFDTDFT
                     TLITHLEQWSDVQVGQSKPKSKS"
BASE COUNT         2020 a         3701 c         4365 g         2040 t
ORIGIN      
        1 cgccgccgct gagacggctg catgccttcg agggggcgtg ccgatacagc ggcacggcga
       61 gcgtcgaggg cgccggcaat agcgtcgcgt gggccatcgc tcgcctgttc ggctttccga
      121 aggcgacacc tagaatcgag gtcgaggtgc aattcacgcg ggtcaacggt gtcgagacgt
      181 ggcgtcggag atttgcaggc gcggccttcg caagcacgca ggaaatcggg cgcggacgca
      241 acgaggggct gctcatcgag cgattcggtc cgcttgcgtt cggtctcgca gtcaccgagc
      301 agggcggccg gctcggcctc gaggtccggc gctggacctt gctcggggtg ccaatgccac
      361 gccggttggc gccttggacg cgcgcgagcg agcacgatgc cggtgaccgc ttcaattttg
      421 acgtcgagat cgggctgccg atcgtcggtc gactggtccg gtatcgcggc cacctggagc
      481 cagtggcgga ctgaggagag gtccagtgcg gttggccgtc gcctgctcag ggatgcaagt
      541 tcgaagggga tctgccatat tcgtccggga tttcaggtgt tgtctggcaa atcgagcggg
      601 aggatgcccc gatgcgacgc ggtctcacga tgggccttgc acttctcctg gcgaccactg
      661 ccctcgatga tcgagccatg gggcagggcc aaggtggctc tccggggacc gaaggcgcgg
      721 cgcccgctgc ggccaaaccc cggagcgacg ggccgaatgt cggcaggtcg gctggtcccg
      781 acgttcgccc caccgcgacg ccacccgtga ggatcaaccc caaggcggcc gctgatccgc
      841 caccaccaag cgcgcccgca aagccgaagg tcgtgaaggc ggcgccgcct gctgtggacg
      901 ccaccgtgaa ggcgccgccg gccaagacgg ctgccgcgtc gccgagcggt ttcatcacgc
      961 gtgcgcgctg cgttcagatg attcgcaaca agtacaagtt catctacgcg gcgaccgact
     1021 ggcagacgaa gatcgatctc tgcgtcacga ccaagggcca gacgttctga tcctggctgg
     1081 tccaccagat cgcccgccga aacgcacgaa aatcccgcgc acagaggcgt gcgcgggcca
     1141 atggatcaac atgggacgtc gctcacgggg ctcagccgcc gcgatagtcc tgcaggcgcg
     1201 tggcgcgcag tccgggcaag ccatgcttgt cgatggagcg ctgccaggac aggaactcgt
     1261 cgactgtcag cttatagcgc tcacaggcct cctcgaggct gaggagcccg ccgcgcacgg
     1321 ccgccacgac ctcggccttg cgccggatca cccaccgctt ggtgtccttc ggcggcaggt
     1381 ccgcgatcgt cagcggactg ccgtctgggc caatgacata ccgcgtgcgc ggcctgaact
     1441 gttgctcggt catgatacgc gaaactctcg ttgacctcac cgacgttacc aaactgcgct
     1501 taaggctggg ctaaacggtc ccacgaatag cgagtgtcgt ttttacgagt gttttcattg
     1561 agtttaacgg gtaagctaaa ctgcaatgaa cgctcgcgtc aatatgggac agagagcctt
     1621 cggcgcggaa ctcacggggc ccggccgatc ctgcatcgat gaggatgacg aattcgaccg
     1681 gctcgaaccc cacgggacgc acgattgcaa tcgccatgcc gggcgcacgc gagctgaata
     1741 tccgaagttc tgagcctggc gaggacgcgc gctcccgcgc tcagtccagg attgggacgc
     1801 ccgggcgtct caggacgagg acgagggcgg cacccgcggc gaggccgccg atatgcgccc
     1861 accaggccgt cgggccgacc tgcggcagaa gcaccatgac gagttgcgtg agaatccagg
     1921 cgccaagcgc ccatgccgcc gagatgttga ggggcacgac gcgcatcacc agcacccaca
     1981 accggacctt ggggtgcagc aggagatagg cgccgatgac gcccgcgacg gcaccggagg
     2041 cgccgaccag cggcagattg gaggtcggca cgacgtaggc gtgagcgagg ccaccggcga
     2101 tgccacagag caggtagaag accgcgaatt tgagatgccc cagggcgtcc tcgacgttgt
     2161 cgccgaacac ccacaggaac agcatgttgc cgacaaggtg gagcggatcg ccgtgcagga
     2221 acatgtaggt gaggagggta agtccctccg ggacggcgag ggcgtcgttc ggcccgttgg
     2281 cggggccgcc gaagatgcgc acgcctaaga gctccgtcgg caccacggcg aagctcgcgg
     2341 cgtaggtggc gcgattgccg gtcacttgca gtgccagcac cacgacgttg atggcgatga
     2401 tggcccaggt cacccaggcg aggcggatat gacggagcgg gttctcgtca tgaatcggga
     2461 cgaagatcgg catgcagttt ccccgcgtat gggcctcggg ccgatcagat cgtttcggtg
     2521 agcagcgaaa gctggcggat atcaggggcc tcggcgtcgg cgagcggtgt cggatagtcg
     2581 ccggtgaagc agtggtcggt gaactgggga ttgcggttgt cgcgacgatc ctggcccatg
     2641 gcgcggtaga tgccagcgac ggagatgaag gcgaggctgt cggcgcccat gtagcggcgc
     2701 atttcctcga tcgatttgtt ggcggcgagc agctcctgct tgcttggcgt gtcgatgccg
     2761 tagaaatcgg ggtgcgtgat gggcgggctc gcaatgcgca tatgcacctc acgtgccccg
     2821 gcgtccttga ggagcgcgac gatcttcatg gaggttgtgc cgcgcacgac gctgtcgtcg
     2881 atcagcacca cgcgagcgcc cttgataacg cccttgttgg ccgagtgctt gagcttcacg
     2941 ccgaaggcgc ggatgcgctg ctcgggctcg atgaaggtgc gaccgacata atgattgcgg
     3001 atgatgccga gctcgaaggg gatgtgcgcg aactcggcga agccgatcgc ggcggggacg
     3061 cctgaatcag gnnnnnnnnn naaccggcac gacgacgtcg gccgagacgc cggattcgcg
     3121 ggcgagctcg gcgcccatga gcttcctgat gccgtagacg ctgcgcccgc cgacgatcga
     3181 atcggggcgg gcgaagtaga tgtactcgaa aatgcacggc cgcgcctggc gcttgggaaa
     3241 cgcgcggatg ctgcgcatgc ccgcggacgt gatcacgacc atctcgccgt tctcgacatc
     3301 gcgctcgtat tcggcgccga cgatgtcgag tgcgcacgtc tcggaggcga gcacgtaggc
     3361 tttgcctatg cggccgatga cgagcgggcg gatgccgacg gggtcgcgca cgccgatcag
     3421 catgtcgctg gtcatgcacg tgagcgcata tgcgccttcg atctggcgga gagcctcgat
     3481 caggcgatcg gtgattgcgc gctcgcgtga gcgtgcagtg aggtggagga tcacctcggt
     3541 gtcggaagtc gcctggaaga gcgctccttc cgagctcagc tcctggcgga gcgtgcgggc
     3601 gttggtgagg ttgccgttgt ggccgatggc aaggcctgtg ccgtcgatgt cggcaaagaa
     3661 gggctggacg ttgcgcagca cgacgtcgcc ggtcgtcgag tagcggttgt ggccgatggc
     3721 gatatggcct ttcagcttgg cgatgacgtc ggccttgttg aagttgtcac cgacgaggcc
     3781 gaggcggcgc tcctgcttga actgcgtacc gtcataggtg acgatgccgc agccttcctg
     3841 gccgcgatgc tggagcgcgt gcagcccgag gacggtgagt gctgcagcgt cgtcgtggcc
     3901 gaagatgccg aatacgccgc actcctcgcg aaggcgatcg tcgccgtggc ggttgaacgt
     3961 aggcgctgga gggagggcat tcggttgcgg ggccggaggg agagcttgag cactcagtcg
     4021 cgtggacgct gcccgaaccg ccatggcttt cctccggctc aacgggacgg ctgccgatcg
     4081 ccgcaccgaa agagtacgtg gccctcggaa gcaggcttcc aaggcatcta tgtggccgtc
     4141 gctatagccg agaatttcga gggccgccag tcacgtcgct gacattgagg tgtgcaagcc
     4201 gtaaagacgg accggcaact cgttcaagtg gtgcggcgat gcatcggcat cacgatcccc
     4261 acaatccggt tgccggcagg ctcgcctggc cgttcgcgta ggagatgccc ggcgcgcgat
     4321 ccctggcgag cagcagcggt ccatcgatgt cggcgaaatc gccttccgtg gcgatgagtg
     4381 cggccggcgc catcgacaag gatgtcgcga gcatgcagcc gaccatgatc ctgaagccga
     4441 gccggtgggc ctcggcgtgc atgcggatcg cctcggtcag tccgcctgtc ttgtcgagct
     4501 tgatgttgac ggcatcgtag cgcccgcgga gcgcgtcgag actatccgtc gtgtgggccg
     4561 attcgtccgc gcaaacgggc acgatgcgct tgatccgggc cagcgcgtcg tcggcaccct
     4621 gtggcagcgg ctgctcaatg agctcgaggc ccgcgtcggc agcagcggcg agcagcggct
     4681 cgagatcagc ggcttgccac gcttcgttgg catctgcaac gaggcgcgca tcaggccgcg
     4741 cctcacggac ggcgcgcata cgctcggcgt caccggcgcc gccgagcttc agcttgagga
     4801 gcggcttgtc ggcatgccgc cgtgccgcat cagccatcgc ctcaggcgtg ccaaggctca
     4861 gtgtgtagca ggtcaggacg ggtcgcggcg cgggaaggcc ggcgaggatg tggacgggct
     4921 gcccggagag gcgcgcctcg aggtcccaga tcgcacagtc gacggcattg cgggcggcgg
     4981 aaggcgggag aattgacgca aggcgggctc tcgcgacctt gggagggccg tacagccggc
     5041 catccagggc ttcaattgcc gatagcgtcc cctcgatcgt ttcgccatag cgcggatacg
     5101 gcgtgcactc gccgcggccc gtcactccat cgatttcggc ctcagcgacg acgacgcggg
     5161 cttcggtgcg ggcgccgcgc gagatcacga attggccgga gatcggccag acctcagacc
     5221 atgctcttgt gaccaaccct gtcatgcaga gcctcccgcc ccgagatgcg gaagccctgg
     5281 aagccgcggt cgatcatctg ctggcagagg cgccgataga catggacgcc gccgacataa
     5341 gggaggaccg tgctgcgctt tccgctcgga tcggggcgta gataccacga cttggcgagc
     5401 ggatagaggg tcttgtcggc gaccgagcgg gagtgtgtca cccagtcgtt ttcggccgtg
     5461 ggatcggctt cgatgatggt cgcgttgcgc cgccgtattt cggcgagaag ggtggcgacg
     5521 agttcgacgt ggtgctcggc tgagaggatc atctggctgc gcacaccggg gctctgcggc
     5581 cccatgagca tgaagaggtt ggggaagcgt gcggtcatca gtccgaggaa tgtgcgcggg
     5641 ccatcggccc aggcggcctt gagcgagacg ccgtcgccgc cctcgatcgc gatgcggtcg
     5701 agcgcaccgg tcacggcgtc gaatccggtg gcgaggcaga ggacgtcgag aggatagagc
     5761 cggtccgagg tctgtacgcc ctcggcctcg atgccgacga tgggatcgga ctcgacatcc
     5821 acgagcgtga cgttctgccg gttgaaggtt tcatagtagt tgctgccgat gagcagccga
     5881 cgcgcgccga tcgggtagcc gcgcggcgtc aggcgctcgg cgaccgcggg gtcccggacc
     5941 acctcgcgaa tcttctcgcg tgcgaactcg gcagcggcgt tgttcgctcc cttgttgatg
     6001 aggatgtcgg tgaacgcgaa ctgaaacgcg accgtgtcgc cggcttgcca ggcagcttcg
     6061 aagcgcgcct ttcgcacctc gggggtgacg tccatggcgc aatgcgtggg gttcacgtac
     6121 ccggagaggc cgaaaggcga aagccgcgcc tgctcgcgct ttgtgcgata gttcgccttg
     6181 tgccggcggg tttcctccgg atcggcgggg gcattccgcg agggaagcac gaaattgggc
     6241 gtgcgctgga agacatagag gtggcgggcc tcgcgcgcga gctcggggat cgcctggatg
     6301 gcggaggcgc cggtgccaat gacgccgacg accttgcccg atacgtccac gccctcgtcg
     6361 ggccagccgg cggtgtgatg ggcggggccc ttgaagcgga ccgggtcgag cacctccggg
     6421 atgagcggga cggacaggtt gcccgtcgcc atgaccagga agcgagcgtg gtggcgcgta
     6481 ccgcggtcgg tggtgatcag ccagcgtgcg gcgctgcgat cgaaccgcgc cgaagagacg
     6541 cgcgtcgaca gctcgatatc gcgacggagg tcgaagcggt ctgcgacgtg ctcgacatag
     6601 cggtggatgt cggcgcggct cgagaagcgc tcgggccagt tccaggactg ctgcagctcc
     6661 tcggagaagg agtacgagta atcgaggctc tcgaagtcgc agcggcagcc agggtagcgg
     6721 ttggcgtacc aggtgccgcc gaggccggcg ttggcctcga tcgcgcgcac gctgagcccc
     6781 atgccgcgga gcttgtggat cgcgtagagg ccggcgaatc cggcgcccac gatcacgacg
     6841 tcgaatgaca gttgggcgtc cgctcgatcg gaatgcgccc gaaccatgaa ttacccctgt
     6901 caacgcaaca cccgacgatt gcaggccaat ctagtcgagc agggcaggtg tcgcggtgat
     6961 cagtttgccg catttggttt cgtggttaac cggcgttgca caggcgccac aaagaaccgg
     7021 gccgaggcgc accgccacga cccggaatgt tacgtcaggg gttcggttgc gtccgtcgct
     7081 cgtcaggcga cggccttcag gacggtcgcg atgcggtcgc ggatctcgcc gatctgcgcc
     7141 tcggagacga tcagcggcgg cgccacgatc agcgtgtcgg cagttacgcg cacgtagagg
     7201 tcgtgatcgt ggtacatgct ctcgatcgcg ttgtagccgc gcaggcccac cttgccgtcg
     7261 acaggatcga ggtcgatacc gcacatgagg ccgatcgggc ggatgtcggc gacgtgcggc
     7321 agccccttca ggctcagcat ggcgtcggcg aacgtcttct cgagcgactt agcccgctcg
     7381 aagagccctt cgttcttata gatgtcgagc gtagcgaggc ccgctgcgac ggccagcgga
     7441 tggccagagt atgtgtagcc gtggaagagc tccaccatat gctcgggccc gctcatgtgg
     7501 gcttcataga tgcccttcct gacgagcacg cccgacatcg gcacggcggc gttggtgacg
     7561 cccttcgcga acgtgatcat gtcggggacg atattgtagc gctcggcggc aaaacatgag
     7621 ccgaggcggc cgaagccggt gatcacctcg tcgaagatca gcaatatgcc gtgcttttcg
     7681 gtaatggcgc gtagcttctc gaggtagccc ttcggcggcg ggatgcagcc cgtggagcct
     7741 gcgaccggct cgacgatcac ggcggcgatg gtcgaggcat cgtggaggcc gatgaggcgg
     7801 gtgagctcgt ccgccgtctc ggaaccccac tccggctcct ggttggagaa ggcctgcttg
     7861 gcgcgattgt aggtcgtcgg caggtgatcg acgccggcga gcatgttgcc gaacatcttg
     7921 cggttggcga ccatgccgcc gaccgatatg ccgccgaaac cgacgccgtg atagccccgc
     7981 tcacggccga tgaatcgcgt gcggctccct tcaccgcggc tgcggtgata ggcgagcgcc
     8041 accttcagcg ccgtgtcgac agcctccgag cccgagttcg cgaacaggca atagtcgaga
     8101 tcgccggggg cgagcagcgt gagcttcgag gagagctcga acaccttggg atgcgcgtac
     8161 tggaagggcg gcgagaaatc gagctcggca gcggtcttct ggatcgcctc gacgatggtc
     8221 ttgcggccgt ggccggcgtt cacgcagaac aggcccgacg tcgcgtcgat caccttgcgg
     8281 ccatcgggcg tcaggtagtg catgtccttg gcgccggaaa agaggcgcgg cttcttcttg
     8341 aacgagcggt tcggcgtgaa cgggagccag tagggctcta gctcgttcgg cttcatgtcg
     8401 agcggtgcca tgggacctcc ttgggtgcgg ggttcaggta tctaaagcag acttggagcg
     8461 gcgggccaag cgcggcagcg gcaaaatcct gcctcggagg ccggacatgc cggtttggca
     8521 ttgcgcggct tggcgcggca gcagcatgca ggttccaact gctcaggctg acgtcaggtc
     8581 agcgaacgcg gtgtcgagca ctgccttgat ttcggcctcg tgccagtact tgactccgac
     8641 ggccggtgtg gcgacggccg gccggccggc gcggcggaca cgtcgaccag tgatcgcttc
     8701 aagtggccgg tcgaggtgtg tgaagtagcc catgttctcg ggctcctcct cgcaccagat
     8761 ggtctcggcg cccgagtgtc gggtcatggc tgtgcgaatc tcgtctgccg gcagcgggtg
     8821 cagttgctcg atccggacga tggcaacttt ttggcccaat ccccgtgccg cgcgctcggc
     8881 ggcgaggtcg tagaagatct tgccagtgca cagaaggacg cggctcgctc cggcgacacc
     8941 ggtgtcgtca atcacgtgac ggaagcccgt accttcggca aagtcggcga gtgtcgagac
     9001 gcacgccttg tgccggagta gtgccttggg tgtcagcacg acgagcggct tcctgaaggg
     9061 ccgagccatc tgccggcgga gcgcgtggaa gaaatttgca ggtgtcgagg cattgacgac
     9121 ctggagattg gcgccggcgc aggcggcgag caggcgctcg ggccggccgg tcgagtgatc
     9181 cgggccgccg ccgtcgaggc cgtgcgggag gaggatgacg agcccgctcg agcgcagcca
     9241 gcggtcctcg ccgcaagcga tgcactggtc gaagacgggt tgcgcgtagt tcaggaactc
     9301 gccgaactgc gcctcccaga tgacgaggcg cgcgggatcg gcgagcgaga tgccgtactc
     9361 gtaggtgagt acggcgtgct cgatcagcgg ggtgttgtgg atctcggcct tggcgaaggc
     9421 gcgggccgcg ggtgccaagg tcagatggcg ctcgccgctc gcctggtcgt gcacctcgag
     9481 atggcgctgg gtgaaagcac cgcgcacgct gtcctgcccg ccgaggcgga ccgaggtgcc
     9541 gtccgagaga aggctcgcga gggcgagcgc ctcgccggtt gcccagttga tgccttcacc
     9601 cgcctcgatc gagcgccgcc gctcgtcgag gaaacgggcg accttgggat cgagcgtggt
     9661 tccgtcgggg agcgatgtga tggcgcggcc gatgtcggtg aggcgggcgg cgggaacgcc
     9721 ggtcaccacg ggggcaagca tggccgcttc gtccccttgg acgaggcctg tccagcggcc
     9781 ttcgaaccaa tcggcttgat tgatcttcca ggatttggcc gcggcgaagg ccgagccgag
     9841 gctcgcgcgc atcgcgtccg tggcgctgtc ggcggcgtcg gtatcgaggc cacgggctgc
     9901 aagctcgtcg gcatagagcg cgcggacggg cggacgcgcg tcgatcgcct cgtacatgcg
     9961 cggctgcgtg aagcgcggct cgtcgatctc gttgtgcccg ggccggcgat agcagacgag
    10021 gtcgatgatg atgtcgctct gaaagcgcgc gcgccagttg gcggcgatga gggcaacacg
    10081 ccagaccgcc tcagggtcgt cgccgttgac gtgtagcacc ggcgcctcga tcaggcgggc
    10141 gatgtcggag gcgccgcggg ccgtgcggcc ttcctcaggc gtagtcgtga aaccgagctg
    10201 gttgttgatg agtacatgga cggtgccgcc gaggcggaag ggcgcgagcc ccgagagctg
    10261 cagcatctcg gcgacgagac cctgtccgga aaagctggcg tcggtgtgga gcaggagcgg
    10321 gaggacgcgg cgggcgccgt cgggaccgag cagcgtctgt ttcgcgcggg cgcggccctg
    10381 cgtgatgatc ggcgcaagct gcagatgcga ggggtgcggc gagacggaga agtgtacggt
    10441 ggagccggcg atctgccggt cgccggagta gccgaggtgg taggagacgt cagaggacgc
    10501 cttcatgcct tcggggatcg cgggtttgcc ctggatctcg gcgatgagcg cggtgagcgg
    10561 cttctccatg atcgtcgcga gcatggcgaa gcggccgcga tgcatgccgc cgacgacgac
    10621 ttcctcgacg ccgagccgcg cactctcggc aatcagtgtc tcgagggcga ccaggaaggt
    10681 ctcggcgcca ccgatgccga agtgcttccc ggccggaatg cgcagattga ggccggactc
    10741 gaaggcatcg acccgagcaa ggagggccag gatgcgccgg cgctcggtgg catcgggtgt
    10801 gacggggtcg gcctgctcgg cctcggcttc gagccagcgg cgccgcgcgc catcgtggat
    10861 gtggccgatc tcccagccga tgctgccaca ataggcccgc tccaggcgct ctgcgagttc
    10921 ggccgcgtcg cggcgggcga ggccgtgggc tgccgggtcg agttcgggga tttcgggacg
    10981 cggcgcgaga ccgagcggat cgaggcgggc acgcaggaag ccgcgatggc gataggcctc
    11041 gaggagagtg gtgagccccg gcgctgccgc cgaggcactg gagcgctgcg ccggccgcgg
    11101 tccggccccg gggcggccat cgaacattgc ttcgagggcg cccggatcgc cgacgcccga
    11161 gaactcctcg agcgcccgca tcaggtcgat cggcggcttg ttcatcggca acgcgcttcc
    11221 gctcaggact tcgacttcgg cttcgactgg ccgacctgga cgtcggacca ctgctcgaga
    11281 tgcgtgatga gcgtggtgaa gtcggtgtcg aagccgccct tgttgaggcc gaggttccac
    11341 atgcccttca ctgcattgcc gacaaacatc gggatgccga gcttctcggc ctcctcgatg
    11401 gcgaggttca cgtccttggt catcaggccc atggagaagc cgtgatcgaa ggtgcgcggc
    11461 agaatgcgcc gcgggaactt gtcttcggtc gccgtattgc ggccggtgct cgcgttgatg
    11521 acgtcgatca tcacgttggc atcgaggccg aacttcacgc ccatgatggc gacctccatg
    11581 gcggcaacca tgttggtcgc tgagaggagg ttgttggcga gcttcatcat ctgcccggtg
    11641 ccgggcttcg ggccgatgta gaaatacttg ccgaacacgc cgaagacggg ctccagcttg
    11701 tcgcactcgg ccttggggcc ggagaccatg caggcgagcg tacccttctc ggcgcccgtg
    11761 acgccgccgg agaccggcgc atcaatctgg atcacgccct tcttgtcgag ctcggcctgg
    11821 atctccttcg ccatgctcgg cccggtcgtc gagaggtcaa cgacgacgcg gcgcttctta
    11881 ccggccgcaa tgccgccggg gccaagcatc acggccttga cgatcggcgg ggtcggcagg
    11941 ctgacgaaga tcgtctcgac ggtcgaggcg acctgctcgg gcgtaccgcc atcgcgggcg
    12001 cctttggcga caacgtcggc gatggccttc tcgtcgacgt cggagacgac gacgtcgtag
    12061 cccgcggcga gtagacgctt caccatcgga ccgcccatct tgccgacgcc gacaaagccg
    12121 atggtgccct ttttct
//
LOCUS       DAORNH010000125        12024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4011_length_12024_cov_2.174785, whole genome shotgun sequence.
ACCESSION   DAORNH010000125 DAORNH010000000
VERSION     DAORNH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4011_length_12024_cov_2.174785"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>368)
                     /locus_tag="PK264_13305"
     CDS             complement(<1..>368)
                     /locus_tag="PK264_13305"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HRD76889.1"
                     /translation="SDVFRQDPVRLIRVFRHCQQLNAKPDFALQALIREGLPLLTRRV
                     QQSPDANVSFRSILEEAGAVYPILSQMHELGVLGRFIPEFDALTCLVQHEFYHRYTAD
                     IHTLHAIRELDNIFTDASPL"
     assembly_gap    369..386
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<387..>1416)
                     /locus_tag="PK264_13310"
     CDS             complement(<387..>1416)
                     /locus_tag="PK264_13310"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76890.1"
                     /translation="NKNFNLIRTYHDAAVGTDDPTTPSIDSTQDQVIDWIVANPGTEL
                     VMGTNNNALAQGGYGRAELSPLSDIDLLFLFPPKAKPLVIKPLQEHLSNEILYILWDC
                     GLKVGHATRTVDETFAEAKNDIQTKTALLESRLIAGAVALYEAFATAYRAFYTEEDPR
                     SYIAARLADQQQRREKYGNTVFLQEPDIKNGVGGLRDYQNAIWMARVKLGISALDELG
                     TQSYLRRNEVRDFQRAYTFLHRVRNELHFQSKRPTDVLALDNQPRIALGLGYTHHDAL
                     GRVEQFMHDYYRAAQTIYRISKLIENRLALTLETPDGSKVSFRDAIRARRMERTKRID
                     GFILRSGEL"
     assembly_gap    1417..1426
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1765..1866
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1867..2724
                     /locus_tag="PK264_13315"
     CDS             <1867..2724
                     /locus_tag="PK264_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRD76891.1"
                     /translation="PNVTGILHMGHVLNNTLQDALIRRARLEGTAACWIPGTDHAGIA
                     TQTMVEKHLKKSEGKGRRDLGREAFLERVWAWRGEKGDIILKQLRELGCSCDWDRTHF
                     TMDPGYSRAVLTSFVHLFRKGNIYRGKRMVNWCPASLTALSDEEVEMRPTKGFIYKLK
                     YDLVTPSGETTHLVVETTRPETIPADVALAVHPDDERYRHLIGQHVWRPLGERVRLPI
                     VADTAVDPVFAAGVLKVTPAHDKVDFEIGQRHRLPVIDALNADGTLNEAAGPELAGLE
                     RFAGRKKAD"
     gene            2755..>2973
                     /locus_tag="PK264_13320"
     CDS             2755..>2973
                     /locus_tag="PK264_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRD76892.1"
                     /translation="MTKTSLDVVAIGNAIVDIIGRCDEGFLAKHGATKGHMRLVDQPT
                     IAKIYDTMGPAVEVSGGSAANTMVGIASF"
     assembly_gap    2974..2983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2984..3749
                     /locus_tag="PK264_13325"
     CDS             <2984..3749
                     /locus_tag="PK264_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HRD76893.1"
                     /translation="GFIGRIADDEFGRIFTHDIRSAGVEFDSKPVAGKAPTARSLILV
                     TPDGERTMNTFLGISPELDQGAVDPAMIARARVVYLEGYLFDRPEAKAAFRQAADLAG
                     KAGAEVALTLSDSFCVDRHRAEFLDLIKTRVDILFANESEITSLYQTASFDEAARRAR
                     TDAKVAVLTRSGKGSLICSGDKAVTIAAVPGIKVVDTTGAGDLYAAGYLYGHATGKSL
                     EMCGRLGSLAAAEVIGHLGARPEVPLARLAADNGLA"
     gene            complement(3883..4740)
                     /locus_tag="PK264_13330"
     CDS             complement(3883..4740)
                     /locus_tag="PK264_13330"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD76894.1"
                     /translation="MADGRRFGWRGWGPDGAPVITALHGTPGSSLKFSVLGTLAAERG
                     YRLIALDRWGYGRTSRHPMPSLSAFAADVAEVADRLRVRRFSIAGVSGGGPYAAAVAA
                     VLGDRIEAAALISPVGPIAGSPATAGLSPFHKLCFRVLPRTPGVLFLAFRGFRRATQL
                     SPWLARRLATGRAAAIDIRTISQPEVGDRLIATFREGLRPGVAGPLTDMSLFSRDWGV
                     RLDAVTAPAVVFIGSDDRNVPIPAAERLARNIRGASLVTLPGEGHLWLATNAPAVLDW
                     IDATRRSGD"
     gene            5025..5735
                     /locus_tag="PK264_13335"
     CDS             5025..5735
                     /locus_tag="PK264_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRD76895.1"
                     /translation="MPSTLGHIKAMAFDTGGTILDWHGGLSRAIAAASASAGLTADWT
                     AVTNDYRRRSLGAIVNQLGPRFNFDDVHRDQLDRVLEAHGLGAIGKSARDEIWRAWFK
                     LDAWPDFPAALERLRTRYPVVSFTLLTLALVVAVSRRNAIVWDGIISCEMIGVYKVRP
                     EAYQTAAKLLQLKPEEILMVACHNFDLDAARGQGFKTCFVRRPLEWGPPGPPDPNPNP
                     GCDLVVDTFEELARISGC"
     gene            5745..6044
                     /locus_tag="PK264_13340"
     CDS             5745..6044
                     /locus_tag="PK264_13340"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD76896.1"
                     /translation="MKERDTLRIAGTRRGGRTVIIVDGRPAAAHEGEMLAAALLAAGI
                     RELRPSPTERTPRGAFCYMGVCQECAVEVDGVRRQACMVRVTPRMSVVLGGGFKT"
     gene            6041..7489
                     /locus_tag="PK264_13345"
     CDS             6041..7489
                     /locus_tag="PK264_13345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76897.1"
                     /translation="MTEGYDLIVAGAGPAGANAAIAAVEQGLSVALIDEADAAGGQVY
                     RRPRVAREARGAGAEAGWRGHDGAAGEELRLRLARSGVVFHASRRIASVSGRFRVDVV
                     GPRGHDALEAPYLIAATGAYERVIPFPGWTTPGVTGLAAATILIESEGMLPGRRVAVV
                     GLGPLLAAVAARIVESGGEVAVVADLASRSDWVKAAWRLTAERPMLIDGWAWLKVIRR
                     ARIPVLHRTGVRRVEGERSVRRLVVGPVGADGAPVPGPHAAFDVDAVVVGHGLVPSGD
                     IPRLLGADLRHDPAGGGWCTLVDGEGRTTLRGLYAVGDGARIAGARLAPLTGRLAGLA
                     VAREIGMISAERFATLAGDTQAEIARLRPVAAAMARLMILKPGLVDGITGGTIVCRCE
                     DIPRSEIDKAIDEGALSLNALKHFTRCGMGPCQGRMCGESVAELLARRSGRSRESLGI
                     WTPRPPLRPIAIDELIGTFSYADIPIPPPSAL"
     gene            7486..8607
                     /locus_tag="PK264_13350"
     CDS             7486..8607
                     /locus_tag="PK264_13350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76898.1"
                     /translation="MRAYDLAVIGGGVHGASAALFAARAGLKVVLIERGALCREASGV
                     NAGTLTIQVTRLALLPHAIGGHQMWATARDWLGHDVGVVVTDGLQLAFSKAEAALLEE
                     RTARRREAGAPLRLVTAKEARAIEPGLSESVLLASHSATDGFAAPYLAGRAFRRALIE
                     TGVAVFEGVYAEGIDRQGAAYAIRLSELSGERVIAATRLVLAGGAWIEEMLGWLGLAV
                     PIAVKINQVAVTERLRPVMRSQIGIASGLLSLRQYPNGTVVIGGGWQGRGDRQKGAHE
                     IEPANLIGNVRLACHAIPALRSGRIVRSWAGIEGKTADELPIIGRLPGHENAWAVGGL
                     HSGFTCGPYVGRELARLITGAESNMPLFPPDRLVARAVR"
     gene            8697..9962
                     /locus_tag="PK264_13355"
     CDS             8697..9962
                     /locus_tag="PK264_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD76899.1"
                     /translation="MLADRYGNALSTRSEAARDAYVTAVDLSLAMLPGAEAGFRAAIA
                     ADEGFALAHAGLARTLQIVAKVPEARAAMARAVELQPGTTARERSHIGVYQEIISGKP
                     PVALEKSKAHIAEWPRDAFALSPSCSVFGLIGFSGLKGREVEQLALLDPLARHYGDDW
                     WFRCVHGFAEVEVGLVDKAKKNLDRSLEMKPDNPNGAHIRGHVYYEAGEPGAGLDYLE
                     RFAASLDKQSPIHCHISWHVALWSLQQGNAERAWAIYSRDLHPGGAWGPPINVLTDSC
                     SFLFRAEMMGERRRPELWREMSEYTSKIFAGTGVAFAAVHGALAHAMAGNGDELEKSI
                     AAARGPAGEVVAALSKGFKAFAAEDYEGAAREFRSTLDRHEQIGGSRAQRDLIEHGLY
                     VALARSGRSEEAHKLLAFRRPIADQARLS"
     gene            9984..>10979
                     /locus_tag="PK264_13360"
     CDS             9984..>10979
                     /locus_tag="PK264_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HRD76900.1"
                     /translation="MASDRITTTHVGSLPRTEMVCDVILAREQGNGDQEGLFDAVLTE
                     AVDAMVERQVQLGIDIVSDGEFSKISYATYIKDRLDGFSGSSPPIMGADLEDFPEYAQ
                     SLLAANKSPIKIVRPLCTGPVRVKNRKPLDDDLRRLRAAVDRHRPTGAFMPAASPGVI
                     AVFQANRYYPTHAAYLEALARAMHEEYKAILAAGFDLQIDCPDLGVGRHLVFKDQSDA
                     AFLRHAEEQVEALNAALVGLQAERMRMHVCWGNYEGPHHKDVPLEHIIGLVLKCKPQK
                     LLIESANPRHAHEWRVFERVKLPDDKVLVPGVIDSTVNYIEHPDLVADRIETFARI"
     assembly_gap    10980..10989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11139..>11390
                     /locus_tag="PK264_13365"
     CDS             11139..>11390
                     /locus_tag="PK264_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD76901.1"
                     /translation="MTSAALEQRLTEWAREVEDFLDRRLSAGSSIPDRLLAAMRHAML
                     GGGKRIRPFLVIESAALFGLDHTVSLIPAAGVECLHGYSL"
     assembly_gap    11391..11411
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11412..>11767
                     /locus_tag="PK264_13370"
     CDS             <11412..>11767
                     /locus_tag="PK264_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD76902.1"
                     /translation="MDDDDMRRGKPTVHLAFDDATAILAGDALQALAFELVASAGVPV
                     ITEQRAELVIGLAKAAGAAGMCGGQQLDLEAETPRGRALSTIEGVSRIQALKTGALIA
                     FSAEAGAILGSASTTE"
     assembly_gap    11768..11777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11778..>12024
                     /locus_tag="PK264_13375"
     CDS             <11778..>12024
                     /locus_tag="PK264_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD76903.1"
                     /translation="GASASDRAALTAYGRALGAAFQIADDLLDVEGDATVVGKATGKD
                     AAAGKATFVSLLGIGGARQRLAEEEAKAVAALAPFGRR"
BASE COUNT         1898 a         3860 c         4125 g         1960 t
ORIGIN      
        1 gaggggagag gcgtccgtga aaatattgtc caattctcgg atggcatgca gcgtatgaat
       61 gtcggcggtg taccgatggt aaaattcgtg ctgcacgagg caggtgagcg cgtcgaactc
      121 cgggatgaag cgtccgagca cgcccaattc gtgcatttgg gacagaatgg ggtagacggc
      181 gcccgcctcc tcgaggatgg atcggaacga aacgttggcg tccggagact gctgcacccg
      241 tcgggtgagc aacgggagac cctcccggat cagggcctgc aaggcaaaat cgggtttggc
      301 gttgagctgc tggcagtgac ggaagacgcg gatgagtcgc accggatcct gtcggaacac
      361 gtcggacgnn nnnnnnnnnn nnnnnngagc tcgccggagc ggagaatgaa tccgtcgatt
      421 cgcttcgtgc gttccatccg gcgggcccgg atggcgtcgc gaaacgagac cttgctgccg
      481 tcgggggttt cgagcgtcag ggcgaggcgg ttttcaatca gcttggagat ccggtaaatg
      541 gtctgagccg cacggtagta gtcgtgcatg aactgctcga cgcgccccag ggcatcgtga
      601 tgcgtgtagc cgaggccgag cgcgatgcgc ggctgattgt cgagggcgag cacgtcggtc
      661 gggcgcttgc tctggaaatg cagctcattc cgcacccgat ggagaaaggt gtaggcgcgc
      721 tggaagtcgc gcacctcgtt gcgtcggagg taggactggg tgcccagttc gtcgagcgcg
      781 ctgatcccga gtttcacccg ggccatccag atcgcgttct ggtagtcgcg caggcctccc
      841 accccgttct tgatgtcagg ctcctggaga aaaaccgtgt tgccgtactt ctcccggcgt
      901 tgctgctggt cggcgagacg ggccgcgatg taggagcggg ggtcctcctc ggtataaaag
      961 gcacggtagg cggtggcaaa ggcctcgtag agggccacgg cgccggcgat cagacgcgac
     1021 tccagcagtg ccgtcttggt ttggatgtcg ttctttgcct cggcaaaggt ctcatccacc
     1081 gtccgggtcg catggcccac cttcaggccg cagtcccaga ggatgtagag gatctcgttg
     1141 gacaggtgtt cctgaagcgg cttgatgacc agcggtttgg ccttgggcgg aaagaggaaa
     1201 agcagatcga tgtcgctcag cggactcaat tccgcccggc cgtagccgcc ctgggcgagc
     1261 gcgttgttgt tggtgcccat caccagctcc gtccccggat tggcgacgat ccagtcgatc
     1321 acctgatcct gtgtcgagtc gatcgacggc gtggtgggat cgtcggtgcc gacggcggcg
     1381 tcgtggtagg tgcggatgag gttgaagttc ttgttgnnnn nnnnnnccct ccagccgcag
     1441 gaaggtcttg caggcggcca gccgctcggc gacgggcaca tcgccgacga agttgaggcg
     1501 ttcgcgcgcg tgtttttgga ggcgaccggt catgaaggga ggggctgcac gtggacgttg
     1561 attcgggagc atcgcccggg ggacgggtgg cattcaacgt ccaagttgcg ggtgggtccg
     1621 gtgggcttcc cacggtgttc gccacgatcc ctcgccctga aaagcccgga tccgccgatt
     1681 ggaagccgct tgcctcgacc gaggtcgggc ggttgcctcg ggggttcaac gtaaccgacc
     1741 atgcccgaga tcaccaaaag ctacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnccga atgtgaccgg gatcctgcac atggggcatg tgctaaacaa caccctgcag
     1921 gacgcgctga ttcgtcgcgc ccggctggag ggcacggcgg cttgctggat tcccgggacc
     1981 gatcacgccg gtattgccac gcagacgatg gtcgagaagc acttgaagaa atccgagggc
     2041 aagggccgtc gcgatctagg ccgggaagcc ttcctcgaac gcgtgtgggc ctggcggggt
     2101 gagaagggtg acatcatcct caagcagctg cgtgagctgg ggtgttcctg cgattgggac
     2161 cgcacgcact tcaccatgga tccgggctac agccgggcgg tgctgacgtc gtttgtgcac
     2221 ctgttccgga aggggaacat ctatcgcggc aaacgcatgg tgaactggtg cccggcgtcg
     2281 ttgacggcgc tctcggatga agaggtcgaa atgcgtccga ccaaggggtt catttacaag
     2341 ctcaagtacg acttggtgac tccgtcgggc gagaccacgc acctggtggt ggaaaccacc
     2401 cgcccggaaa cgattccagc cgacgtcgcg ctggccgtcc atcccgacga cgagcgttac
     2461 cggcacctga ttggccagca cgtctggcgt ccgttgggcg agcgcgtgcg actgccgatc
     2521 gtggccgata cggcggtgga tccggtgttt gccgcaggcg tgctcaaggt cactccggcc
     2581 catgacaagg tggactttga aatcggccag cgccaccgcc tgccggtgat cgacgcgttg
     2641 aacgccgacg gcaccctcaa cgaggcggcg ggtcccgaac tggccggact ggagcgtttc
     2701 gccggccgca agaaggccga ctgagccgac gcttcaccac tcagggggta tcgcatgacc
     2761 aagacttcgc ttgacgtcgt cgccatcggc aacgccatcg tcgacatcat cggccgctgc
     2821 gacgagggct ttcttgccaa gcacggcgcc accaaggggc acatgcgcct cgtcgaccag
     2881 ccgacgatcg ccaagatcta cgacacgatg gggccggccg ttgaggtctc cggcggctcg
     2941 gccgcgaaca ccatggtggg gatcgcatcc ttcnnnnnnn nnngggcttc atcggccgca
     3001 tcgccgacga cgagttcggg cgcatcttca cgcacgacat ccgctcggcc ggggtcgagt
     3061 tcgacagcaa gccggtcgcc ggcaaagcgc cgaccgcgcg ctcgctgatc cttgtcacgc
     3121 ccgatggcga gcgcaccatg aacactttcc tcggcatcag tcccgagctc gaccagggcg
     3181 ccgtcgatcc ggcgatgatc gcgcgcgcgc gcgtcgtcta cctcgagggt tatctgttcg
     3241 atcggcctga ggcgaaggct gcgttccggc aagcggccga tctcgcgggg aaggccggcg
     3301 ccgaggtggc gcttacgctc tccgacagct tctgcgtcga tcgccaccgc gccgagttcc
     3361 tcgacctcat caagacgcgt gtcgacatcc tgttcgccaa cgagagcgag atcacctcgc
     3421 tctaccagac cgcctcgttt gacgaggccg cccgccgcgc ccgcaccgac gcgaaggttg
     3481 cggtcctcac gcggagcggc aaaggctcgc tgatctgctc gggcgacaag gcggtgacga
     3541 tcgcggccgt gccgggcatc aaggtcgtcg atacgaccgg tgcgggtgac ctctatgcgg
     3601 ccggctacct ctatggccac gcgacgggaa agagcctcga gatgtgcggc cgtctcggca
     3661 gccttgccgc cgccgaggtc atcggccacc tcggtgcccg gcctgaagtc cccctcgccc
     3721 gcctcgccgc cgacaacggg ctcgcgtgag ggcgctcgcc ggaggcaggt gtcttcttgc
     3781 ggtagtgtgg ctgtcgccgc tcccgcatcg aacggtgaag cggtgtcacc tcggcccgac
     3841 atcgcctcaa gtcaagacgc ggggcggccc ggtcgcgtgg agttagtccc cactccggcg
     3901 cgtcgcgtcg atccagtcga gcaccgccgg cgcgttggtg gcgagccaga ggtggccttc
     3961 gccggggagc gtcacgagcg acgcgccgcg gatgttccgg gccaggcgct ccgccgccgg
     4021 aatgggcacg ttccggtcgt cgctgccgat gaagaccacg gccggtgccg tgacggcatc
     4081 gaggcgcaca ccccagtccc ggctgaatag ggacatgtcg gtgagcgggc cggcaacgcc
     4141 cggccggagc ccttcgcgga aggtcgcaat gagacgatca ccgacctccg gctgactgat
     4201 cgtgcggatg tcgattgcgg cggcccgtcc ggtggcgagc cggcgcgcga gccacggact
     4261 cagctgggtc gcgcgcctga agccgcggaa ggctaggaac agcacgcccg gcgtccgcgg
     4321 cagcacgcga aagcagagct tgtggaaggg actcaatccg gcagtagcgg gcgatccggc
     4381 gatcggaccg acggggctga tcagcgccgc ggcttcaatg cgatctccta gcaccgctgc
     4441 aacggccgcc gcatagggcc cgccacccga gacgccggcg atcgagaagc ggcgaactcg
     4501 gaggcggtcc gcgacctcgg cgacgtccgc ggcaaaagcg gagagcgagg gcatcgggtg
     4561 gcggctggtc cttccgtatc cccagcggtc gagggcgatc aggcgatagc ccctctcggc
     4621 ggcgagcgta ccgagcaccg agaatttcag cgacgacccc ggcgtgccgt ggagcgcggt
     4681 gatgaccggc gcgccgtcag gtccccagcc gcgccatccg aaccgacggc cgtccgccaa
     4741 tgcgatttcc tggaccggtg gaagctcgcc ggccgctcgc gggtgcgtcc cgtcagacat
     4801 agtgcatggc gagaacgtag agaacgaggg ccacaagcgc gcagcgccga tagaagcggc
     4861 aggtggagca tgcccaggct gcagccagtc gctcgcggag cgtcgaggag ggggtgtgat
     4921 tgatggtcgc cgtccgtgct cgccatgtgg tccgatccgc gagcccgcgt tgacagtctg
     4981 ccggcacggg tccgatatgg ttggagtaag ttgaggagtg aaagatgccg tcaactctcg
     5041 gccatatcaa ggccatggcc ttcgacacgg gcgggaccat cctcgattgg cacggcggcc
     5101 taagccgggc gatcgccgcg gccagcgcgt ccgcgggcct cacggccgat tggaccgccg
     5161 tcaccaatga ctaccgccgc cgctcgctcg gtgcgatcgt caatcagctc ggcccgcgct
     5221 tcaattttga cgacgtgcac cgcgaccagc tcgatcgcgt gctcgaggcg cacgggctcg
     5281 gcgcgatcgg caaatcggcg cgcgacgaga tctggcgggc ctggttcaag cttgacgcct
     5341 ggcccgattt tcccgccgcg ctcgaacggc tgcgaacacg ttatccggtc gtctccttca
     5401 cgctcctaac gctcgcgctc gtggttgccg tctcgaggcg caacgcgatc gtctgggacg
     5461 gcatcatcag ctgcgagatg atcggcgtct acaaggtgcg ccccgaagcc taccagaccg
     5521 cggcgaagct gctccagctg aagcccgaag aaatactgat ggtggcctgc cacaatttcg
     5581 atctcgacgc ggcgcgtggc caaggcttca agacctgctt cgtgcgccgt ccgctcgaat
     5641 gggggccacc cggcccgccc gacccgaacc ccaatcctgg ctgcgatctg gttgtcgaca
     5701 cgttcgaaga gctcgctcgg atctctggtt gctgagggcg ccccatgaag gagcgcgaca
     5761 cactcaggat cgccggcact cgccgcggcg ggcggacggt gatcattgtc gacggccggc
     5821 cggctgcggc gcatgagggc gaaatgctgg cggcggcgct gctggcggct ggcatccgcg
     5881 agttgcgccc gagcccgacg gaacggacgc cgcgcggcgc cttctgctac atgggtgtct
     5941 gccaagagtg tgccgtcgag gtggacgggg tgaggcggca ggcctgcatg gtgcgtgtga
     6001 cgccgcgcat gagcgtggtg ctcgggggcg ggttcaagac gtgacggaag gctacgatct
     6061 gatcgttgcc ggcgcaggcc cggctggtgc aaatgcagcg atcgctgccg tcgagcaggg
     6121 gctgagcgtc gccctgatcg acgaggctga cgcagcgggt gggcaggtct atcggagacc
     6181 gcgcgtcgct cgcgaggctc gaggggccgg cgccgaggcc ggatggcgag gccatgacgg
     6241 cgccgccggc gaggagctcc gactgaggct tgcccgctcc ggtgtcgtct tccacgcgag
     6301 ccgccgcatc gcgagtgttt caggccggtt ccgcgtcgat gtcgtcggac cgcggggcca
     6361 cgatgcgctc gaggctcctt acctcatcgc cgcgacgggc gcctatgagc gcgtcatccc
     6421 ctttccgggc tggaccacac ccggcgtgac tggtctcgcc gctgcgacga ttctcatcga
     6481 gagcgagggg atgctgcccg gccggcgcgt cgcggtcgtg ggccttggcc cgcttctggc
     6541 ggcggttgct gcgcgcatcg tcgagagcgg cggtgaggtc gcggttgtcg ccgaccttgc
     6601 gagccgcagc gattgggtga aggccgcgtg gcgcctcact gccgagcggc ccatgctcat
     6661 cgacggctgg gcgtggctga aggtgatccg ccgcgcccgg atcccggtgc tgcatcggac
     6721 cggcgtgcgg cgcgtggaag gcgagcgctc cgtgcgccgc ctcgtcgtcg gcccggtcgg
     6781 ggcggacggt gcgccggtgc cgggaccaca tgcggcattc gatgtcgatg ccgtcgttgt
     6841 cggccatggg ctcgtgccct cgggcgacat tccgcgcctg ctcggtgccg atcttcgcca
     6901 tgatccggca ggcggcggct ggtgtacgct tgtcgacggc gaggggcgga ccacgctgcg
     6961 cggtctctat gccgtcggcg acggtgcgcg gatcgcgggc gctcgtctag cgccgctcac
     7021 cggtcgcctc gccgggctcg ccgtcgcccg tgagatcgga atgatctcgg ccgagcgctt
     7081 tgccacgctc gcaggcgaca cccaggctga gatcgcgcgt ctgaggccgg tcgcggccgc
     7141 aatggcgcgc ctcatgatcc tgaagccggg cctcgtggat ggcataaccg gaggcactat
     7201 cgtctgccgc tgtgaggaca tcccgcggag cgagatcgac aaggcgatcg acgagggcgc
     7261 tctcagcctc aacgctctga agcacttcac gcgctgcggc atggggccgt gccaggggcg
     7321 catgtgcggc gagtcggtcg ccgagcttct ggcgcggcgc tcgggcagat cgcgcgagag
     7381 cctcgggatc tggacgccgc gcccgccgct ccgcccaatc gccatcgacg agctcatcgg
     7441 cacattcagc tacgccgaca tcccgatccc gccgccgagc gcgctatgag ggcctacgat
     7501 cttgcagtga tcggaggcgg cgtgcacggc gcatcggcgg cgctgtttgc cgcgcgagcc
     7561 ggcctgaaag tcgtgctgat cgagcgtgga gccctctgtc gcgaagcctc gggcgtcaac
     7621 gccggcaccc tgacgatcca agtgacgcgg ctcgccctgc tcccgcacgc gatcggcggg
     7681 catcagatgt gggcgacagc ccgcgactgg ctcggccacg atgtcggcgt cgtcgtgacg
     7741 gacggcctgc agctcgcctt ctcgaaggcc gaggcagcgt tgctcgagga gcgcaccgct
     7801 cgccgacgcg aggccggcgc gccgcttcgc ctcgtgacgg cgaaggaggc gcgcgccatc
     7861 gagccggggc tctccgagag cgtgctgctc gcctcacact ccgcgacaga cgggttcgcg
     7921 gcgccctatc ttgccggacg cgcgttccgg cgggcactga tcgaaactgg cgtcgccgtg
     7981 ttcgaaggcg tgtatgccga gggcatcgat cggcagggcg cggcctatgc gatccgcctc
     8041 agcgagctaa gcggggagcg cgtgatcgcc gccacgcgcc tcgtcctcgc gggcggtgcc
     8101 tggatcgagg agatgctcgg ctggctcggc ctggccgttc cgattgccgt gaaaatcaac
     8161 caggtggctg tgacggagcg gctcaggccg gtcatgcgca gccagatcgg catcgcctcg
     8221 gggctgctgt cgctccgaca atacccgaac ggcacggtgg tgatcggcgg cggctggcag
     8281 ggccgcggcg atcgccagaa gggggcccac gagatcgagc ccgcgaacct catcggcaac
     8341 gtgcggctcg cctgtcatgc gatccctgcg ctgcggtcgg gccggatcgt gcgctcctgg
     8401 gccggcatcg agggcaagac cgcggacgag cttcccatca tcgggcggtt gcctggccac
     8461 gagaacgcct gggcggtcgg cggcctgcac tcgggcttca cctgcggtcc ctacgttggc
     8521 cgcgagcttg cccgcctcat caccggtgcc gaaagcaaca tgccgctttt tccaccggac
     8581 cggctcgtcg cgcgtgcggt gaggtgaccg catgccacaa tcggcgcgat cgcgtcattg
     8641 cccgcgccgc tcgaaccgga atagcatgtt gctggacgag gacatcggag aaggccatgc
     8701 tcgcggaccg ttatggaaat gcgctgtcga cacgctccga ggcggcgcgc gatgcctatg
     8761 tcacggctgt cgacctgtcg ctcgcgatgc tgccgggcgc tgaagccggg ttccgcgcag
     8821 cgatcgctgc ggatgaggga tttgcgctcg cacacgccgg cctcgcgcgc acactgcaaa
     8881 tcgttgccaa ggtccccgag gcgcgggcgg ccatggcgcg cgccgtcgag cttcaacccg
     8941 gcaccacggc gcgcgagcgg agccatatcg gtgtctatca ggagatcatc tcggggaagc
     9001 cgcccgtcgc cctcgaaaag tcgaaagctc acatcgccga gtggccgcgg gacgccttcg
     9061 cgctgtcgcc gagctgcagc gtgttcgggc tgatcggctt ttccggtctc aagggtcgcg
     9121 aggtcgagca gctcgccctc ctcgatcccc ttgcacggca ctatggcgac gattggtggt
     9181 tccgctgcgt ccacggcttt gcggaggtgg aggtcgggct cgtcgacaag gccaagaaga
     9241 acctagatcg atctctcgag atgaagcccg acaatccgaa cggtgcccac atccgcggcc
     9301 acgtctacta tgaggcggga gaacccggcg ccgggctcga ctatctcgag cggttcgcgg
     9361 cctcactcga caagcagtcg cccatccatt gccacatcag ctggcatgtg gcgctctggt
     9421 ccctgcagca ggggaacgcc gaacgggcgt gggcgattta cagccgcgat ctgcaccccg
     9481 gcggcgcctg gggaccgccg atcaatgtgc tcacagattc ctgctcgttc ctattccgcg
     9541 ctgagatgat gggtgagcgg cgccggcccg aactctggcg cgagatgagc gagtacacct
     9601 ctaagatctt cgccggcacc ggcgtcgcct tcgccgcggt ccacggcgcg ctcgcccatg
     9661 cgatggcagg aaacggcgac gaactggaga aatcgatcgc ggctgcgcgg ggaccggccg
     9721 gcgaggtcgt cgcggcgctt tccaaaggtt tcaaggcgtt tgcggccgag gactacgagg
     9781 gtgctgcgcg cgagttccgc tcgacgctcg atcgccatga gcagatcggc ggcagccgcg
     9841 cccagcgtga cctgatcgag catggcctct acgtggcgct cgcccgatcc ggccgaagcg
     9901 aggaggccca caagctcctt gcattccgcc ggccgatcgc cgatcaggct cggctttcct
     9961 gacatcaccc cgcgaggcga tccatggcca gcgaccgcat caccacgacc catgtcggca
    10021 gcctgccacg caccgagatg gtgtgcgacg tgatcctggc ccgcgagcag ggcaacggcg
    10081 accaggaggg cctcttcgac gcggttctca ccgaggccgt cgatgccatg gtcgagcggc
    10141 aggtgcagct cggaattgat atcgtctcgg acggtgagtt ctcaaagatc agctacgcga
    10201 cctacatcaa ggaccgcctc gacggcttct cgggctcgag cccgccgatc atgggcgccg
    10261 atctcgagga cttccccgag tacgcgcaga gcctgctcgc cgccaacaag tcgccgatca
    10321 aaatcgtccg cccgctctgc acgggaccgg tgcgcgtcaa gaaccggaag cctctcgacg
    10381 acgatctccg ccgccttcgg gcggccgtcg acaggcaccg cccgacgggc gcgttcatgc
    10441 cggccgcctc gccgggcgtg atcgccgtgt tccaagcgaa ccgctactat ccgacacatg
    10501 cagcctacct cgaggcgctc gcccgcgcca tgcatgagga gtacaaggcg atcctcgcag
    10561 ccggcttcga cctgcagatc gattgcccgg atcttggtgt cggccgccac ctcgtcttca
    10621 aggaccagag cgatgcggca ttcctgcgcc atgccgagga gcaggtggag gcgctgaacg
    10681 cggcgctcgt cggcctgcaa gccgagcgca tgcgcatgca cgtgtgctgg ggcaactatg
    10741 aaggcccgca ccacaaggat gtgccgctcg agcacatcat cggacttgtc ttgaagtgca
    10801 agccgcagaa gctgctgatc gaatccgcca atccgcgcca tgcacatgag tggcgcgtgt
    10861 tcgagcgggt gaagctgccg gacgacaagg tactcgtgcc gggtgtgatc gattcgaccg
    10921 tcaactacat cgagcacccc gacctcgtcg ccgatcgcat cgagacattt gcgcggatcn
    10981 nnnnnnnnng tcggccgcga gcgcgtgata gccggcacgg attgcggctt ctcgaccttt
    11041 gccggtcgca gcctcgtcga cccgaagatc tgctttgcga agctcgaggc gctggtgcaa
    11101 ggcgccgaga tcgcgacgcg gcgcctgtgg gggcggggat gacaagcgcc gcgctcgagc
    11161 agcgtctcac tgagtgggcg cgtgaggtcg aggattttct cgatcgccgt ctttccgcag
    11221 ggagcagcat tcctgaccgg ctgctcgccg ccatgcgtca cgccatgctc ggtggcggca
    11281 agcgcatccg cccgttcctc gtcatcgaga gcgcggcgct gttcggcctg gaccataccg
    11341 tctcgcttat tcctgcagcg ggcgtcgaat gcctgcacgg ctattctctc nnnnnnnnnn
    11401 nnnnnnnnnn ngatggacga cgacgacatg cgccgcggca agccgacggt gcatttggcc
    11461 ttcgacgatg cgacggcgat cctggcgggt gatgcccttc aggcactcgc cttcgagctc
    11521 gtcgcatcgg ccggagtgcc cgtaatcacg gagcagcgcg cggaactcgt gatcggcctg
    11581 gcgaaggccg caggtgccgc cgggatgtgc ggcggccagc agctcgatct cgaggccgaa
    11641 acgccgcgcg ggcgtgcgct ctcgaccatc gagggtgtgt cgcggatcca agccttgaag
    11701 acgggcgcgc tgatcgcctt ctcagctgaa gcgggcgcga ttctcgggtc ggcgtcgacg
    11761 accgagcnnn nnnnnnnggt gcatccgcgt cggaccgggc ggcgctcacc gcctatggca
    11821 gggcgctcgg tgccgcgttc cagatcgccg acgatctcct cgacgtcgaa ggcgatgcga
    11881 ccgtcgtcgg caaggcgaca ggcaaggatg ccgcggccgg caaggcgaca ttcgtctcgc
    11941 tgctcggcat cggtggggcg cggcagcggc ttgccgagga ggaggcgaag gcggtcgcgg
    12001 cgctggcacc tttcggccga cgcg
//
LOCUS       DAORNH010000126        11919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4057_length_11919_cov_2.758092, whole genome shotgun sequence.
ACCESSION   DAORNH010000126 DAORNH010000000
VERSION     DAORNH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4057_length_11919_cov_2.758092"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            628..1680
                     /locus_tag="PK264_13380"
     CDS             628..1680
                     /locus_tag="PK264_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD76904.1"
                     /translation="MAQATGQSMTASALEEAITLQQAKKLIQCMAHEQSFLLLSPPGV
                     GKSEMVYQAAREARLPCRSLLGTQIAPEDVSGVPRIVGERSVFCPPRILLPESPEPFC
                     LFLDELPAAAPDVQKAFYSLLLERRLGEHELPKGTWVVAAGNRVQDRALVRSLSSALV
                     NRVTILHVKVDAEEWLAWGRANGVRADILNVITFMPDALMRPVPVEPQPFSTPRAWAL
                     LSRSLDMAEASGILTRETRRALAFGRLSPEDAAVYCALAEEAIGAMHPLEHYIEEPGA
                     LPKGDAARWFVLNCIRQRVQAGTLTGLTPRQVNKFLRTLPSEHQLTLVGDLVGRWSDL
                     GADPAMLDLLKTVTKL"
     gene            1677..>2835
                     /locus_tag="PK264_13385"
     CDS             1677..>2835
                     /locus_tag="PK264_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76905.1"
                     /translation="MSATPEHLPRPVPAKGPSSDDQKQLQRIREGLRLVTAPLPYLSG
                     LAAAVRVSLDDRVPTMGVFASGRMIANAAFAKKLSERELVFVLAHELLHLALRTHDRA
                     RGSERLEFNYAHDYIINDILREELGFTKVPAGGLDMAGARHKSAEEIVLDMRRNADRI
                     RSKTQVFDGSDTTVGRMFGRGQDRSGKGQGKPGKGAGKGSEAEKGDTPPDLDAAGDVL
                     DERRERELFPNEAGEIKAREEAMKELAAKGLAIARASGALKGTRGLSPGDQTEMVSAL
                     RGLYQTPWRVILQRWMEAVAPGERTFSRPSRRGEMGHDIVLPGRRREGWMLNLVLDTS
                     GSMSDEIPRALGAIADFCDAVGVDQVRIIQCDVGVTVDELVEPGTLATYRVS"
     assembly_gap    2836..2861
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2862..3054
                     /locus_tag="PK264_13390"
     CDS             <2862..3054
                     /locus_tag="PK264_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76906.1"
                     /translation="SDMSPAMDRLADDPLVRAAIVITDGDITYPTDAMPYDVLWVLPM
                     GASRAFNPPYGRTILMLPG"
     gene            3074..3460
                     /locus_tag="PK264_13395"
     CDS             3074..3460
                     /locus_tag="PK264_13395"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HRD76907.1"
                     /translation="MKVIQELVAHFERQQKLSSRQVRALLDQGYLANDAPPTIMELCE
                     TVGAVYYFRVTGAVTGMVWGVDTYTGDSAVAAASVHAGAVKDGETAIIKVTVLAPLNQ
                     YQGSVRNGVTSHEFGRFGTAYKVERV"
     gene            3564..5369
                     /locus_tag="PK264_13400"
     CDS             3564..5369
                     /locus_tag="PK264_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD76908.1"
                     /translation="MTTNSLRWMLLDRAFTTLSVLLALAMPVSAEPTFGIAMHGPPKH
                     GPATGHLPYVNPDAPKGGRLTLGQLGAFDSLNPLIIKGQAATGIRDFVIESLLGRALD
                     EPFSLYGLLAESIDVPADRSAITFKLRPEARFSDGTPVTADDVIASHTMLSAKGLPYM
                     RAYYRKVARVVRLGERDVRFEFDAKGDREIPLIIGLMPILPRHKFTLDTIEQTTLEPI
                     VGSGPYLIAAVDRGRAITFRRNPDYWGRDLWLMKGRFNFDEVRYTYYSDAEVMFQDFK
                     AGHIDFRLEDDPKRWATDYEFPAVRSGEIVRESVPIALPAGMSAFVFNTRRPMFADRN
                     VRRALILAFDAERINRTFYHGLFARTESYFDRSTLASRGRPADARERALLAPWIDRLE
                     PDILEGRFRIPGSGGQSRDNLRQAIALLEKAGYVARGSRLFSTRNGQPLTIEILTRSR
                     AQDRLAGAYVEDLRRLGITVAIRQVDATQYQERLRTYDFDMIQTIWHTSLSPGNEQTN
                     RFGSSSAREERTLNYAGVADPAVDAMIGALLEAVEPADFTSAVRALDRLLLSGDYVVP
                     LFHLERQWVAYWRRLAHPAASSLYGYGLDTWWIRR"
     gene            5474..5806
                     /gene="trxA"
                     /locus_tag="PK264_13405"
     CDS             5474..5806
                     /gene="trxA"
                     /locus_tag="PK264_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRD76909.1"
                     /translation="MTAMVNDKNFDAEVLKSTQPVLVDFYAEWCPPCKAMAPALEKVA
                     QEMKAVVKIVKLDVDASPQTTQKYNIQAMPTMIMFKGGKPAATQVGALIQKSQLETWI
                     KKQLGAGA"
     gene            complement(5885..6047)
                     /gene="ssrS"
                     /locus_tag="PK264_13410"
     ncRNA           complement(5885..6047)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK264_13410"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6130..6753)
                     /locus_tag="PK264_13415"
     CDS             complement(6130..6753)
                     /locus_tag="PK264_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRD76910.1"
                     /translation="MSSVAAVLLAAGSARRFAGGAKLFAEIDGEPLVLHAARALAASR
                     CERLVVVTGPEADRVKAALAALPRLTPELVHNDRHAEGIGGSIAAGVRALPRTATAVL
                     IVQADMPGLTSTLVDGLIGLQEERAALIGIAPVVFPTLADGRQRSPVLWPARFRDKLE
                     ALTGDQGAKGLIAAARGETAPLLVDDPRLIADIDTIEELEAYRAGKA"
     gene            complement(6750..7787)
                     /locus_tag="PK264_13420"
     CDS             complement(6750..7787)
                     /locus_tag="PK264_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRD76911.1"
                     /translation="MSEPGHAPDAKAEIDVLKAAATWLKRDGKVALATVIDTWGSAPV
                     PVGGQMAIAADGRFQGSVSGGCIEGEVIVEAGEILSGGKPTTMKFGVADETAWRVGLP
                     CGGEVAVYLERIEGERGAGLIDTAISARETRRGLVVKTDLATGDKQLFERGGNLPDEI
                     AGRFKSAKSGLVEEAGRQAFVQALVPPARLLIVGATHIAQLLCGIARIAGYDLIVVDP
                     RTAFAAADRFEGVRLETEWPQDAIPKIGLDPYTAVIALSHVGHIDDEALKLALRSDCL
                     YVGALGSKRNHAKRTERLTAAGFSKDEIALIHCPIGLDIGAQSPAEIAVSIMAEVVRA
                     VRGTKRQTAAA"
     gene            complement(7874..9100)
                     /gene="carA"
                     /locus_tag="PK264_13425"
     CDS             complement(7874..9100)
                     /gene="carA"
                     /locus_tag="PK264_13425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRD76912.1"
                     /translation="MSTANLAKPRAWVAEAKTARLVLADGTVIEGQGLGATGTAVGEV
                     CFNTAMTGYQEILTDPSYAGQIITFTFPHIGNVGTNAEDTETSNLAARSGVRGAVLRA
                     NVTGPSNYRSVERLDRWLAARGIIAIAGVDTRALTARIRSEGMPNGVIAHDPGRHFDI
                     DRLKAEAKAWPGLDGMDLVPDVTAGQSYSWDESRWTWGKGYGRQSTPEFHVVAIDYGL
                     KRNILRCLVSAGCRVTVVPAKTSAADILARKPDGVFLSNGPGDPAATGEYAVPVIKEL
                     VASGLPVFGICLGHQMLAIALGGRTHKMHQGHHGANHPVKDLTTGKVEITSMNHGFTV
                     DRASLPKGVEETHVSLFDKTNCGLSLAGKPVFSVQYHPEASPGPEDSHYLFVRFVNLM
                     RTRAGRPPVKEGKALT"
     gene            9330..10160
                     /locus_tag="PK264_13430"
     CDS             9330..10160
                     /locus_tag="PK264_13430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD76913.1"
                     /translation="MLRQRANQAEQQARIEVELANRVKSEFLSNMSHELRTPLNTIIG
                     FSKLIGEHDRRKIGDKDIVEYARLIQDAAAHLLTIINDILDISKLHAGRFSLDAREVD
                     LEELIGTLIVPFSSQAEAAGVKIEHHLDRDVVPVRGDAGKLRQALSNILSNAIKFTKP
                     GGSVFIELAATPENGAMVSIRDTGIGMSEEEVRIALTPFGQADASHSRWREGTGLGLP
                     IAKALIELHGGGLQIQSAKSMGTEVLVELPSRDFVGLVGTNVALLGQVNSPAETGIRS
                     "
     gene            10180..>11919
                     /locus_tag="PK264_13435"
     CDS             10180..>11919
                     /locus_tag="PK264_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRD76914.1"
                     /translation="MTTERTTDGMGRIVSVNGSKAIVLLDSRSDGAQRGPSQRPEMGT
                     LLGIDTANTVVLAIVSALSVPVPAQRDGDAELWIAELGLVGELKKGPDGKPFTFNRGV
                     TTYPTLGDRARVATKGELEIAFVGDARNSVRVGAIRQDSTIPAMIRVDDLLGKHFAIL
                     GTTGTGKSCTTALILRSILMANPAAHIVLLDPHNEYATAFSEFAEVVSPRNMQLPYWL
                     LTFEELVEVLIGGANIADKKPEIELLQELIPIAKARYGSTRARESQAVRTRVLDAGRF
                     TVDTPVPYRMSDLTSLIDERMGKLENKKDLSPYRNIKSRIDMLSHDPRYAFMFGSLTV
                     FDGMAQILGRIFRVPVNNKPITILELTGLPTEIINVVVSVICRLTFDFALWGEGKVPV
                     TVVCEEAHRYVPVNASLGFEPCKRAIAKIAKEGRKYGASLCIVTQRPAEIDPTILSQC
                     NTVFALRMSNDRDQEIVQSAISDTGSGLLEFLSALGQREAIAFGDGVTLPVRIRFDEL
                     PKTAMPRSSTAKFSEQWKESIGDEAFLEQIVERWRNSASGAGGDHGRQLAMLADAMGI
                     ADDLYTGILTSGEA"
BASE COUNT         2029 a         4127 c         3778 g         1959 t
ORIGIN      
        1 ggcgctctct catcctgatt gtcgctgagt tctcaccttg attgtcgccg cccagctgtc
       61 cccgtcgctg acgtccatcg ctcgagcccg cgcatgatat cggcgtcgaa ccaaacctga
      121 attagatcga ggacccggcc tttgccgacc ttctcctcga cccgggctgc cagctgcgca
      181 agcgggatcg tgtatcgcgg taggggcggc cgttgctggc cgccccccgc atggatccgg
      241 acgtgccgat ttcctgtatc cggcaactgt attcttttga gcccgggtcc gtgaaggaca
      301 agccctcacg ggcgtcaata cgttcgcttc gcgacatcgt tcgctttgcg cccgggcctc
      361 gctgtgagat tgcgcgcctt ccgcgcgcgg gcccagaccc gctcggtgaa agcttctccc
      421 gaacccctat ttttcaatac caagaagggg aggctcggag gatctcctcc gggcagagcg
      481 tgaggcggca gcctcgctcg cgaagcgagt gacgcgcaca agcgcgagtg gcactgttca
      541 gaaacgcgcc gcatttttgc ttcactcgag cataagcgtg aagcgtatcg aatccgggtc
      601 tccacccgcc gcagcaggga gcgcgccatg gcgcaggcaa cagggcagtc gatgacggcg
      661 agcgcgctcg aggaggcgat caccctccag caggcgaaga agctcatcca gtgcatggcg
      721 cacgagcaga gctttctcct tctctccccg cccggcgtcg gaaaatcgga gatggtctac
      781 caggccgccc gcgaggcgcg cctcccctgc cgctcgctgc ttggcacgca gatcgcgccc
      841 gaggacgtct cgggcgtgcc gcgcatcgtc ggcgagcgga gcgtgttctg cccgccgcgc
      901 atcctgctgc cagagagccc cgagccgttc tgcctgttcc tcgacgagct gcccgccgct
      961 gcccccgatg tgcagaaggc attctattcg ctgctactcg agcgccgcct cggcgagcac
     1021 gagttgccga aaggaacgtg ggtcgtcgcc gccggcaacc gcgtacaaga ccgcgcgctg
     1081 gtgcgctcgc tctcttccgc cctcgtcaac cgcgtgacca tcctgcacgt gaaagtcgat
     1141 gccgaggagt ggctcgcctg gggccgcgcc aatggcgtgc gcgccgacat cctgaacgtc
     1201 atcaccttca tgccggacgc gctgatgcgc ccggtgccgg tcgagccgca gcccttctcg
     1261 acgccgcgcg cctgggcgct cctctcacga tcgctcgaca tggccgaggc gagcggcatc
     1321 ctcacgcgtg agacacgccg cgcgctcgcc ttcggccgcc tgtcgccgga ggacgccgcc
     1381 gtttactgcg cgctcgccga ggaggcgatc ggcgccatgc acccgctcga gcactacatc
     1441 gaggagccgg gtgcgctgcc gaagggcgat gccgcgcgct ggttcgtgct caattgcatc
     1501 cgccagcgcg tgcaggcggg tacgctcacg ggcctcacgc cgcgccaggt caacaagttc
     1561 ctgcgcacgc tgccgagcga gcatcagctg acgcttgtcg gcgacctcgt cggccgctgg
     1621 agcgacctcg gtgccgaccc ggccatgctc gacctcctga agacggtgac aaagctgtga
     1681 gtgccacgcc tgagcatctc ccgcggcccg tgccggcgaa aggcccgagt tccgacgacc
     1741 agaagcagct gcagcgcatc cgcgaggggc tgcggctcgt cacggcaccg ctcccgtatc
     1801 tttctgggct tgccgcagcc gtgcgcgtct cgctcgacga ccgcgtgccg accatgggcg
     1861 tgttcgcctc gggccgcatg atcgcgaatg cggcgttcgc caagaagctc agcgagcgcg
     1921 aactcgtctt tgtgctggcc cacgagctgc tgcatctggc actgcgcacc cacgaccgtg
     1981 cgcgcggctc cgagcggctc gagttcaact acgcgcacga ctacatcatc aatgacatcc
     2041 tgcgcgagga gctcggcttc accaaggtgc cggcgggcgg cctcgacatg gcgggcgcgc
     2101 ggcacaagtc ggccgaggag atcgtcctcg acatgcgccg gaacgccgac cgcatccgct
     2161 cgaagacgca ggtcttcgat ggctcggaca cgaccgtcgg acgcatgttc ggccgcggcc
     2221 aggaccggag tggcaaaggc cagggcaagc ccgggaaggg tgctggcaag gggagtgaag
     2281 cggagaaggg cgacacgccc cctgatctcg acgcggccgg agacgtcctc gacgagcgcc
     2341 gcgagcgcga gctcttcccg aacgaggccg gcgaaatcaa agcccgcgag gaggcgatga
     2401 aggagctcgc cgccaagggc ctcgccatcg cgcgggcctc tggcgcgctg aagggcacgc
     2461 gcggcctttc gcccggcgat cagacggaga tggtgtcggc gctgcgcggg ctctaccaga
     2521 cgccgtggcg cgtcatactg cagcgctgga tggaggccgt ggcgcccggc gagcgcacct
     2581 tcagccggcc atcgcgccgg ggcgagatgg gccatgacat cgtgctcccc ggccggcgcc
     2641 gcgagggctg gatgctcaac ctcgtgctcg atacctcggg ctcgatgagc gacgagatcc
     2701 cgcgcgcgct cggcgcgatc gcagactttt gtgatgccgt tggagtcgat caggtgcgca
     2761 tcatccaatg cgacgtcggc gtcaccgtcg acgagcttgt cgagcctggc acgctcgcca
     2821 cttaccgcgt ctccgnnnnn nnnnnnnnnn nnnnnnnnnn ncagcgacat gtcgcccgcg
     2881 atggatcgcc tcgccgacga cccgctggtg cgtgcagcga tcgtcatcac cgacggtgac
     2941 atcacctacc ccacggacgc aatgccctac gacgtactct gggtgctgcc gatgggcgcc
     3001 tcgcgcgcgt tcaacccgcc ctacgggcga accattctga tgctgcccgg ctgagccggc
     3061 gaaggagtga acgatgaagg tcatccagga gctggtcgcc catttcgagc gccagcaaaa
     3121 gctatcctcc cgccaggtcc gcgcgctgct tgaccagggc tatctcgcca acgatgcgcc
     3181 gccgacgatc atggagctct gcgagacggt cggcgcggtc tactatttcc gcgtgacggg
     3241 cgccgtcacg ggcatggtgt ggggtgtcga cacatacacg ggcgacagcg cggtcgcggc
     3301 ggcatcggtt catgcgggcg cggtcaagga cggcgagacg gccatcatca aggtgaccgt
     3361 gctcgcgccg ctcaaccagt accagggctc ggtgcgcaac ggcgtgacga gccacgagtt
     3421 cggccgcttc ggcacggcct ataaggtcga gcgggtgtga ggtcactccg gtacgcccca
     3481 ctgcggccga tttgctcggc actctctcgc cagctgagag accccgcgct atggctcggg
     3541 tcgaacgctg cgcgagaggc ccgatgacga cgaactcact tcggtggatg ctgctcgatc
     3601 gggcattcac gacactctcc gtcctgctgg ccctcgcaat gccggtaagc gccgagccga
     3661 ccttcggcat cgccatgcat gggccgccga agcacggacc ggcgacgggg catctccctt
     3721 acgtcaatcc ggatgcgccg aagggcgggc ggctcaccct cggtcagctc ggcgccttcg
     3781 acagcctcaa tccgctgatc atcaagggtc aggccgcgac cggcatccgc gatttcgtga
     3841 tcgagagcct gctcggccgc gctctagatg aacccttctc gctctatggc ctgctcgccg
     3901 agagcatcga tgtgccggcc gaccgcagtg ccatcacgtt caagctccgc cccgaggcgc
     3961 gcttctcgga cggcaccccg gtcaccgccg acgacgtgat cgcgtcccac acgatgctgt
     4021 cggccaaggg cctcccctac atgcgcgcct actaccgcaa ggtggcgcgc gtcgtgcggc
     4081 tcggcgagcg cgacgtgcgc ttcgagttcg acgccaaggg cgaccgcgag atcccgctca
     4141 tcatcgggct gatgcccatc ctgccgcgcc acaaattcac gctcgacaca atcgaacaga
     4201 cgacgctcga gccgatcgtc ggcagcgggc cgtacctgat cgccgccgtc gatcgcggcc
     4261 gcgcgatcac gttccggcgc aatcccgatt actggggccg cgatctctgg ctgatgaagg
     4321 gccgcttcaa cttcgacgag gtgcgctaca cctactattc agatgccgag gtgatgttcc
     4381 aggacttcaa ggcaggtcac atcgatttcc gcctcgagga tgatccgaaa cgctgggcaa
     4441 ccgactacga gttcccggcc gtgcgtagcg gcgagatcgt gcgcgagagt gtgccgatcg
     4501 cgctgccggc cggaatgtcg gccttcgtct tcaatacccg ccggccgatg tttgccgacc
     4561 ggaacgtgcg ccgcgcattg atcctcgcct tcgacgccga gcgcatcaac cgcaccttct
     4621 atcatggcct cttcgcgcgg actgaaagct acttcgaccg ctcgaccctc gcctcgcgcg
     4681 gccggcctgc tgatgcgcgc gagcgcgccc tcttggcacc ctggatcgat cggctcgagc
     4741 ccgacatcct ggagggccgc tttcgcattc ccgggagtgg cgggcagagc cgcgacaacc
     4801 tccgccaggc gatcgccctc ctcgagaagg ccggatatgt cgccaggggc agtcgcctct
     4861 tctccacccg gaacgggcag ccgctcacga tcgagatact gacgcggagc cgcgcccagg
     4921 accggctcgc cggcgcctat gttgaggacc tccgtcgcct cggcatcacg gtcgccatcc
     4981 gccaggtcga cgccacccag taccaggagc gtctgcgaac ttacgacttc gacatgatcc
     5041 agacgatctg gcacacgtcg ctttcgcccg ggaacgagca gaccaaccgc ttcggttcat
     5101 cctccgcccg cgaggagcgc acgctcaact atgccggcgt cgccgatccc gctgtcgatg
     5161 caatgatcgg cgcgctgctc gaggccgtgg agccggctga tttcacctcg gccgtgcgcg
     5221 cgctcgaccg gctgctgctc tcgggcgact atgtcgtgcc cctcttccat ctcgagcgcc
     5281 aatgggtcgc ctattggcgc cgcctcgcac accctgccgc cagctcgctc tacggctatg
     5341 gcctcgatac ctggtggata cgccgttagc agccctggct tgccttgacg gggtgcgggc
     5401 gcgcacctaa ttacccgtga gctttctccc gtccgatcga tggcgatctc acgggcaaac
     5461 tcaggagatc gtcatgacgg cgatggtgaa cgacaagaat ttcgacgccg aggtgctgaa
     5521 atcgacccag cccgtgctgg tagatttcta tgccgagtgg tgcccgccct gcaaagcgat
     5581 ggcgccagcc ctcgaaaagg tcgcgcagga gatgaaggcg gtcgtcaaga tcgtcaagct
     5641 cgacgtcgac gcgagcccgc agacgacgca gaagtacaac atccaggcca tgccgacgat
     5701 gatcatgttc aagggcggca agccggccgc gacccaggtt ggcgcgctca tccagaagtc
     5761 gcagctcgag acctggatca agaagcagct cggcgccgga gcctgatgag ccgcctcagc
     5821 gctgaacgac atcagggccc gcgagggccc tgaattgttt gtggcgaacg caacgattgc
     5881 atagtgcagg gccgcatgag ccgttggagc ggctgatccc gctgggtccc tacaaaagta
     5941 ggtgggcgcc gtgtgtccgg aaccacggtt ccggccaggg acagctccct cagcgaaatt
     6001 tataggcccc ggggttcaaa agcccctgac gcactccgca gccctgcgag tcgcaaagta
     6061 gtcgatcgcc gttgcttttc caatacggtc atacggccct cggacacggc cctgggccac
     6121 catcccgttt caggccttgc cggcgcggta ggcctcgagc tcctcgatcg tgtcgatgtc
     6181 ggcgatgagg cgcgggtcgt cgacgaggag tggcgccgtc tcgccgcgcg ccgcagcaat
     6241 caggcccttc gccccctggt cgcccgtaag ggcctcgagc ttgtcgcgga agcgcgcggg
     6301 ccagaggacc gggctccgct ggcggccatc ggcaagggtc gggaaaacga caggcgcaat
     6361 cccgatcagc gcggcccgct cctcctgaag cccgatcagg ccatcgacga gggtggatgt
     6421 gagccccggc atgtcggcct gcacgatcag gactgccgta gccgtgcggg gaagcgcgcg
     6481 cacgcccgcg gcgatggagc cgccgatccc ctcggcatgg cgatcgttgt ggacaagttc
     6541 aggggtcaac cgtggcagcg cggcgagcgc ggccttcacg cgatccgcct ccggtcccgt
     6601 cacaacgacg aggcgctcgc agcggctcgc ggcaagggcc cgcgcggcgt gaagtaccag
     6661 cggttcaccg tcgatctcgg cgaagagctt ggcgccgccg gcaaagcgtc gcgccgagcc
     6721 tgcggcaaga aggacagccg cgacgctgct catgccgccg cagtctgccg cttggtgccg
     6781 cgcacggcgc gcacgacctc ggccatgatt gagactgcga tctcggccgg cgattgcgcc
     6841 ccgatatcga gaccgatcgg acagtggatg agggcaatct cgtccttgct gaagcctgcc
     6901 gccgtgagcc gctcggtgcg cttcgcgtga tttctcttgg agccgagcgc gccgacgtag
     6961 agacaatcag accgtagcgc aagcttcaac gcctcgtcgt cgatgtgacc gacatgggag
     7021 agcgcaatca cggcggtgta cgggtcgagt ccgatcttcg ggatggcgtc ctgcggccac
     7081 tcggtctcga ggcgcacgcc ttcgaagcga tcggcggcgg caaaggccgt gcgcggatcg
     7141 actacaatga gatcgtagcc ggcgatgcgg gcaataccgc agagcagctg cgcgatatgc
     7201 gtcgcgccga cgatcagcag ccgcgccggc ggcaccagcg cctgcacgaa ggcctgccgc
     7261 cccgcctcct cgacgaggcc gctcttggca ctcttgaagc ggccagcgat ctcgtccggc
     7321 aggttgccgc cgcgctcgaa gagttgctta tcccccgttg cgagatcggt cttgacgacg
     7381 aggccgcgcc gcgtctcgcg cgcactgatc gccgtatcga tgaggcccgc gccgcgttcg
     7441 ccttcgatac gctcgaggta gacggcgacc tcgccaccgc aagggagccc gacccgccag
     7501 gcggtctcat cggcgacgcc gaacttcatg gtcgtcggct tgccacccga gaggatctcg
     7561 cccgcctcga cgatcacctc gccctcgatg cagcctcccg agaccgagcc ctggaagcgg
     7621 ccgtcggcgg caatcgccat ttgcccgccg accggcacgg gtgccgagcc ccaagtgtcg
     7681 atgacggtgg cgagcgcgac cttgccgtcg cgcttcagcc aggtcgccgc agccttcaga
     7741 acgtcgatct cggctttggc gtcgggtgcg tgtccaggct cggacatggg ggcctcgcag
     7801 gcgtgatacg ggtttgcgcg aaagctagcg agcgcggctc acccaagcaa gcgaggccag
     7861 cgcactgctt gacctacgtc aacgccttgc cttccttgac gggcggccgg cctgcgcgcg
     7921 tgcgcatcaa attgacgaag cgcacgaaga gatagtggct gtcctccggg cccggcgacg
     7981 cctcgggatg gtactgcacc gagaacacgg gcttgccggc gaggctcaac ccgcagttgg
     8041 tcttgtcgaa gagagaaacg tgcgtctcct cgacgccctt cggcaggctc gcccgatcga
     8101 ccgtgaagcc gtggttcatc gaggtgatct cgaccttgcc ggtcgtcagg tcctttacgg
     8161 gatgattggc gccatggtgg ccctgatgca tcttgtgcgt ccgcccgccg agcgcgatcg
     8221 ccagcatctg gtggccgagg cagatgccga acacgggcag acccgaagcg acaagctcct
     8281 tgatcacggg aacggcatac tcgcccgtcg ctgccggatc gcccggacca ttcgacagga
     8341 aaacgccgtc gggctttctc gccaggatgt cggctgcgga ggtcttcgcc ggcacgaccg
     8401 tcacccggca gccggcgctc acgagacagc gcaggatgtt gcgcttcaac ccatagtcga
     8461 tggcgacgac gtggaactcg ggcgtcgact ggcggccata acccttgccc catgtccaac
     8521 gtgattcgtc ccagctgtag ctctggcccg ccgtcacgtc gggaacgaga tccatgccgt
     8581 cgaggcccgg ccaggctttc gcctctgcct tcaagcggtc gatgtcaaag tgccgtcccg
     8641 gatcatgggc aatgacaccg ttcggcatac cttcggagcg gatacgcgcc gttagcgccc
     8701 gcgtatcaac acctgcaatc gcgatgatgc cgcgcgcggc gagccagcga tcgagacgct
     8761 cgaccgagcg atagttcgac ggtcccgtga cattggcccg caggaccgcc ccgcgcacgc
     8821 ccgaccgggc ggcgaggttg gaggtctcgg tatcctccgc attggtgccg acgttgccga
     8881 tgtgcgggaa ggtgaaggta atgatctgcc cggcatagga ggggtccgtc aggatctcct
     8941 gatagcccgt catcgcggta ttgaagcaca cctcacccac ggccgtacca gtcgcgccga
     9001 gcccctgccc ctcgatgacg gtgccatccg cgagcacgag gcgtgcggtc ttggcctccg
     9061 ccacccaggc gcgtggcttg gccaggtttg ctgtcgacat ggctcgctcc ttgatcgggc
     9121 gggaagctag tcgtgacgcc ccgaccggtc aacgcctctt ggccaccaaa atgtctcaag
     9181 cgtcaagccc ggttaaccct gtgttatccg tccgcaacta gtcttgctgc gtcgtaaggg
     9241 cgtgcggcga gcactcgatg aacggtccga tcagcactgg caggcaggcc accctgatgg
     9301 gtgaataatc ggcgaagctg ggtgatgccg tgctgcgcca gcgcgccaat caggccgagc
     9361 agcaggctcg catcgaggtg gagctcgcga accgggtcaa atccgagttc ctttccaaca
     9421 tgagccacga gctgcgcacg ccgctcaata cgatcatcgg cttttccaag ctcatcggcg
     9481 agcacgaccg acgcaagatc ggcgacaagg acatcgtcga gtacgcccgc ctcatccagg
     9541 acgcggcagc ccacctcctc accatcatca acgacatcct cgatatctcg aagctgcacg
     9601 cgggacgctt ttcgcttgat gcgcgcgagg tcgatctcga ggagctcatc gggacactca
     9661 tcgttccctt ctcctcccag gcggaggctg cgggcgtgaa gatcgagcat cacctcgatc
     9721 gcgacgtcgt gcccgtgcga ggcgatgccg gtaaactccg ccaggcgctc tcgaacatcc
     9781 tctccaacgc catcaagttc acgaagcccg gcggctcggt cttcatcgag ctggccgcca
     9841 cccccgagaa cggcgccatg gtctccatac gcgacactgg tatcggcatg agcgaggagg
     9901 aggtgcgaat tgccctcacc ccgttcggcc aggctgatgc cagtcactcc cgctggcgcg
     9961 aaggtacggg cctcggcctc cccatcgcca aggcgctgat cgaactccac ggcggcggcc
    10021 tgcagatcca gagcgccaag agcatgggaa ccgaagttct cgtcgaactg ccgagccgcg
    10081 acttcgtcgg gctggtcggg accaacgtag cacttctcgg gcaggtgaat tcgcctgccg
    10141 agacgggtat ccggagctga tcgagcgatg caacccaaga tgaccacaga gcgcaccacc
    10201 gatggcatgg gccgcatcgt ctccgtgaac gggtcgaaag cgatcgtcct gctcgacagc
    10261 cgcagcgacg gggcccagcg cggtccctcg caacgacccg aaatgggcac gctgctcggc
    10321 atcgacacgg cgaacaccgt cgtgctggcg atcgtctcgg cgctctcggt accggtgccg
    10381 gcacagcgtg atggcgacgc cgagctctgg atcgccgaac tcggcctcgt cggcgaattg
    10441 aagaaggggc cggacggcaa gcccttcacc ttcaaccgcg gcgtcactac ctacccgacc
    10501 ctcggtgacc gagcccgcgt cgcgaccaag ggcgaactcg aaatcgcctt cgtcggcgat
    10561 gcccgcaact ctgtccgggt cggcgcgatc cgccaggatt cgacgatccc cgcaatgatc
    10621 cgcgtcgacg atctcctcgg caagcacttt gccatcctcg gcaccacggg caccggcaag
    10681 tcgtgcacga ccgcactcat cctgcgctcg atcctgatgg cgaaccccgc cgcccacatc
    10741 gtgctgctcg acccgcacaa cgagtacgca accgccttca gcgagttcgc cgaggtcgtc
    10801 tcgccgcgca acatgcagct cccctactgg ctcctcacct tcgaggagct ggtggaggtg
    10861 ctgatcggcg gggccaacat cgccgacaaa aagcccgaga tcgagcttct ccaggagttg
    10921 atcccgatcg ccaaggcgcg ctacggctcg acccgtgcgc gggagtcgca ggcggtgcgc
    10981 acgcgcgtcc tcgacgccgg ccgctttacc gtcgacacgc ccgtccccta ccgcatgtcg
    11041 gatctgacga gcctcatcga cgagcgcatg ggcaagctcg agaacaagaa ggacctctcg
    11101 ccctaccgca acatcaagag ccggatcgac atgctctcgc atgatccgcg ctacgccttc
    11161 atgttcggct cgctcaccgt gttcgacggc atggcgcaga tcctcggccg catcttccgc
    11221 gtgcccgtca acaacaagcc gatcaccatc ctcgagctca ccgggctgcc gaccgagatc
    11281 atcaacgtcg tcgtctcggt gatctgccgc ctgaccttcg acttcgcgct ctggggcgag
    11341 ggcaaggtcc ccgtgacagt cgtctgcgag gaggcgcacc gctacgtgcc ggtgaacgcc
    11401 agcctcggct tcgagccctg caagcgggcc atcgccaaga ttgccaagga aggccgcaag
    11461 tacggcgcct cgctctgcat cgtgacgcag cgcccggccg agatcgaccc gaccatcctc
    11521 tcccagtgca acaccgtgtt cgcgctgcgc atgtccaacg accgtgacca ggagatcgtg
    11581 cagtcggcga tctcggatac gggctcgggc ctcctcgagt tcctctcggc actcggccag
    11641 cgcgaggcga tcgccttcgg agatggcgtg acgctgccgg tgcgcatccg cttcgacgag
    11701 ctgccgaaga cggcgatgcc gcgctcctcc actgcgaaat tttccgagca gtggaaggaa
    11761 tcgatcggcg acgaagcctt cctcgagcag atcgtcgagc gctggcggaa ctcggcgagc
    11821 ggcgctggcg gcgatcacgg ccggcagctc gccatgcttg cagatgccat gggcatcgcc
    11881 gacgacctct acaccggcat cctgaccagc ggcgaggcc
//
LOCUS       DAORNH010000127        11777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4117_length_11777_cov_2.695786, whole genome shotgun sequence.
ACCESSION   DAORNH010000127 DAORNH010000000
VERSION     DAORNH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4117_length_11777_cov_2.695786"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PK264_13440"
     CDS             complement(<1..1080)
                     /locus_tag="PK264_13440"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD76915.1"
                     /translation="MLRALVAALAAIFFLAGPAAAGPNDIVLIFANETYVGSVPKAGF
                     AHRDGEEMRRAARDVLAVPESRIIVIKDATSGTFQDWVESGGGVGPVLAQLPIRRDST
                     IYVYYSGHGMPARKSGASEAESFLLPVDTPPAQVARRGLAVSDLRAALAAVQKLKAPD
                     GRVVLWLDACFTGSSGGGDLVSDSRSVRISTKAIMAAEPKLVEIAAASFEQEALWDAK
                     RGHGVFTDTLLDGLYGAADADKDGHVTADELHRFLVDHVEERVARLFPSPRRFQTPML
                     LGDRSIRIASVTGAVRDPALRAEMQIRCSTTKASSDISEIRAFLSDCGPACPCRADLQ
                     KRIGELERSSSTCIGEGRELARLEKL"
     gene            complement(1227..2504)
                     /locus_tag="PK264_13445"
     CDS             complement(1227..2504)
                     /locus_tag="PK264_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76916.1"
                     /translation="MAIVNLVVQSAATSSPDERARYVEELQRIADGEVSRDTAATAAD
                     AMDVVKRLPRRPKRLDKLRVVVIRPWHLYAAAAGIAVAIPFSLVSVASVILSLWHYLV
                     SDLWRTAVQPLLAQSVRSAFWVFCAACLAPGLAGLYASLRLTRDRKLRFDPAMGSIWV
                     WQDKQFAHGIQQRIHLIDAELKARRGPPKGFERGSPLRYSLKFFGVIFASLLAIAAVV
                     IGFIILEASWQRNANTISEMTRTIETGSLTGRHLADAHFCRAFAWERTRQDELARNDL
                     DRALKADSTHLAALLARASLSAKEWRRGSPYANRSEALSASFQDLSRAIELDPTLFAA
                     RYNRALLRLSKMDRMGAEADFKAAEEILLSATSASSGWTISRARHRFDQARTKPLRRL
                     HVAPQLPASCPLARRPDDPIFGFFWWIGEWLDG"
     gene            complement(2714..3574)
                     /locus_tag="PK264_13450"
     CDS             complement(2714..3574)
                     /locus_tag="PK264_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76917.1"
                     /translation="MTVDIDAIRRHIGTKMIEEDIATSSPPRCMIATFNRYEPAPKAG
                     ERIAPGWHLGYFMQATPPAVLAEDGLPTTWGVLPKMPFPRRMYAGSSIHFHDDLRVGD
                     AITRETEFTGADVRKGSTGPLIFTTQARRIYTRRGLAIVEESHTVFREDVKAGEKNAT
                     PKRDAPPAGLPWSRTITADPVSLFRYSALTFNPHRIHYDRTYAMTVEGYPGLVVHGPY
                     SQQCLIDLARDNAGGRRITRYSQRARAPLFDIAPFSVVGGPSEDGKGAKLWAVTPEGT
                     IAMEATVAYG"
     gene            complement(3588..4007)
                     /locus_tag="PK264_13455"
     CDS             complement(3588..4007)
                     /locus_tag="PK264_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRD76918.1"
                     /translation="MSAETIAKPIPQPSQESKPFWDGLLAGKLLLQTCGSCGKVRHYP
                     RPICPSCYSFEVRWSEAKGRGLVHSWTVTHHPFHPGFKGEVPFTLVTVDLPEGVRMQA
                     PLRGAEAASLKLGAPVRLVIERPTAELALPAFVLDKA"
     gene            complement(4009..>4254)
                     /locus_tag="PK264_13460"
     CDS             complement(4009..>4254)
                     /locus_tag="PK264_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRD76919.1"
                     /translation="KKGEGGAFVASGAIRLGGKLPINTHGGLLSQSHMVGMNHVVELV
                     RQLRGDAGKAQVKDAEVGLVTGYGDMGDGSIAIMRRG"
     assembly_gap    4255..4330
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4331..5152)
                     /locus_tag="PK264_13465"
     CDS             complement(<4331..5152)
                     /locus_tag="PK264_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HRD76920.1"
                     /translation="MTLKEAVAVTGVGETAYTRNSGKSVAALQLEASLAAIRDAGLSP
                     KDIDGIIPYSNSPVVAEDFITNFGLDDLRFSATTPMGGASAVAAIQCAALAISAGLCR
                     HVLLPIGRNGASQGRIGLRVAEMPQFRLIGEHEMPVGAIGPAQLYAPMARRHMELYGT
                     TSRQFGEIAVSTRANAILNGRAMMMKPIAIEDHQASRMISDPLRLLDCSLESDGGAAV
                     VVSAASLARDLKQRPVYVMGMAEGHPDSPSAITQRPDLTRLGIAKAAPKAFAMAGV"
     gene            complement(5518..5689)
                     /locus_tag="PK264_13470"
                     /pseudo
     CDS             complement(5518..5689)
                     /locus_tag="PK264_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031943491.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5763..6086
                     /locus_tag="PK264_13475"
     CDS             5763..6086
                     /locus_tag="PK264_13475"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRD76921.1"
                     /translation="MGTTPPVRSPVMIAAAGWLVWYKEGLGAAFITWVAALGLNAAWS
                     WPFFGRQWNGRALVDILALLAAIAIFIAMTWTPARTAAMLLVPYLLRVAFASGQNLEI
                     WQLNR"
     gene            6165..>7212
                     /locus_tag="PK264_13480"
     CDS             6165..>7212
                     /locus_tag="PK264_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76922.1"
                     /translation="MEGGKEGGKRIAVIGSGISGLSAAWLLAKRHHVSLIEADMRPGG
                     HSNTVDACLGRCTIPVDTGFIVYNTASYPNLIALFEHLGVPTVATDMSFAVSLDGGRY
                     EYSGSSLGSLFGQRSNLFRPSHWRMLADTIRFFREAQGAAALNASPAVTLGAFLAGRG
                     YSEAFIKRHILPMAAAIWSTPSRAVLDFPLAAFVRFFANHGLLQVANRPEWRTVVGGS
                     REYVTRLLADFDGEVLLGAGARRITRHGDGVTVETARGEQPYDGCVIATHADDALSLL
                     ADPSKDERDLLGAFRYIANRAVLHTDRSQMPRRTGLWSSWNYLGSGAGLHGNLAVTYW
                     MNKLQPLGGSAPDLF"
     assembly_gap    7213..7222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7223..7541
                     /locus_tag="PK264_13485"
     CDS             <7223..7541
                     /locus_tag="PK264_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD/FAD-binding protein"
                     /protein_id="HRD76923.1"
                     /translation="PPPTFEPSSIVSAFDYSHPMFDARAMQAQRRLWDLQGCRSTWFA
                     GSYFGFGFHEDGIQAGLAAAEAIGGVRRPWSVAEESGRIHITPRAPHEDASACARYRE
                     AAE"
     gene            7538..8335
                     /locus_tag="PK264_13490"
     CDS             7538..8335
                     /locus_tag="PK264_13490"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HRD76924.1"
                     /translation="MTASGSAIFEGTVVHKRLSPKPHAFAYRVFTLCLDLDEIASLDR
                     ELRLFSVNRRNVVSFWDSDLGPGGPEAAAEKARALLDHCGLGVYGARIELVCYPRLLG
                     YVFNPLSVYFCRNSDGRLGAVVYEVSNTFSERKSYVIPIEGESGIISQQCVKDMYVSP
                     FTKAAGSYGFHCVAPSERVVIGVNFREAGRPVLKTHFAGVRRPLSDRALLSAVTRHPL
                     MTMKVMAAIHFEAARLWAKGVPVVTRHASPAYSYTRVNSSRGTPAHV"
     gene            8328..9575
                     /locus_tag="PK264_13495"
     CDS             8328..9575
                     /locus_tag="PK264_13495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HRD76925.1"
                     /translation="MSDEPSVGRPAAAPAATAPLGGLASVGTLPLRLLDGLLRRRGIG
                     RLRVRLPGGAEGVIGEAGSPVEASLEVRAYAAVWKVWRRGALGFAESYMDGDFETADL
                     RRLFELYLANEPALTRAFPRLNLTRGLDRRFHASRQNTYSGSRRNIAEHYDLGNAFYR
                     LWLDPSMLYSSGIYTSDTASLEEAQAEKIGRILAALELLPEQSVLEVGCGWGALAAAM
                     AERGARVRAITISAEQLAEARARIGRAGLSEVVETVFEDYRDTVGGFDRLVSIEMIEA
                     VGEENWPLFFGTIADRLNPGGTAVIQAITIREDAFASYRANPDFIQRYIFPGGMLPTV
                     KLMHQRASEAGLSFEIVERFGHSYALTLAEWRRRFEHAWPQIAALGFDERFRRMWRYY
                     LTYCEIGFERGLIDVGLYRLRKA"
     gene            9632..10123
                     /locus_tag="PK264_13500"
     CDS             9632..10123
                     /locus_tag="PK264_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 family protein"
                     /protein_id="HRD76926.1"
                     /translation="MPQDMAPASIQRVPRFFDLTSFLHGRTRAWGVFEDRFGNVRRRL
                     TVDMLGRWEGGEFVLEEQFVYDGVSSEQRTWRVVPEANGRFRAACSDCVGEAIGQCAQ
                     DSVRMSYRFRLKLDARDLVVTLDDRLYQIGPDHAINRARMSKWGVKLGELSLLFERIP
                     DRK"
     gene            complement(10127..10870)
                     /locus_tag="PK264_13505"
     CDS             complement(10127..10870)
                     /locus_tag="PK264_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD76927.1"
                     /translation="MPSPWRTAWITGASTGIGRELTLKLAGRGVRVAASARSEARLAE
                     LAALHPSLLPVPLDVTDRSRTAAAHARISDELGAIDLAVLNAGVWQPMPASALDATRI
                     AESMNVNYVAIANALELLIPAMTKAGKGHIALVASMAGYRGLPNAVAYAPTKAAVISL
                     AEVLRLELPRHGIKVSLVNPGFVETPMTSINTFPMPFIIKADDAAERILKGLERGRFE
                     IVFPWQLATIMKLLRILPNALFLRLAGRL"
     gene            complement(10887..11537)
                     /locus_tag="PK264_13510"
     CDS             complement(10887..11537)
                     /locus_tag="PK264_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HRD76928.1"
                     /translation="MMIPITHHPDEATLVSYAAATLSEPLAAVVAAHLAMCPRCRSEV
                     ADLERLGAALMFADTASPSETPLAPRRRPPDTASFVGKPVQPIDERLPAPLALAYGLA
                     WDEIPWRRLGPGVWHHKLPLCQPAEGDLRLLRIAPGRAMPEHGHGGSELTLVLEGSYS
                     DVTGSYGRGDVQDVDSGVDHQPIVGKEAECICLIASERPARFKSLIGRLTQPWTGL"
     gene            complement(11534..>11777)
                     /locus_tag="PK264_13515"
     CDS             complement(11534..>11777)
                     /locus_tag="PK264_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRD76929.1"
                     /translation="ARVLSYRDRPDLVAERQRQLRVQAILKELPPEQVEVVTLAFVDG
                     LAHSEIADRLSLPLGTVKSRIRLAYQKLRAALEDIR"
BASE COUNT         1968 a         3765 c         3915 g         2043 t
ORIGIN      
        1 gagcttctcc aggcgggcga gctcgcggcc ttcgccgatg caggtcgagg aggagcgctc
       61 gagttcgccg atgcgcttct gcaggtctgc gcggcatggg caggctggcc cgcagtcgga
      121 aaggaaggct cgaatttctg agatatcgct cgatgccttc gtcgtcgagc agcggatctg
      181 catctcggct ctcaatgccg gatcgcgcac cgcgccggtg acgctcgcga tgcggatcga
      241 gcggtcaccg agcagcatcg gcgtctggaa gcggcgcggg ctcgggaaga ggcgcgcgac
      301 gcgctcctcg acgtgatcga ctaggaagcg atgcaactcg tccgccgtga cgtgaccgtc
      361 cttgtcggcg tctgcagcgc catagagccc gtcgagcagc gtgtcggtga acacgccgtg
      421 gccgcgtttg gcgtcccaga gcgcctcctg ctcgaagctt gccgcggcga tctcgacgag
      481 cttcggttct gccgccatga tcgccttcgt cgagatgcgg accgagcgcg aatccgagac
      541 gaggtcgcct ccgcccgacg agcccgtgaa gcaggcgtcg agccagagga cgacgcggcc
      601 gtcgggcgct ttcaatttct gtacggccgc gagtgccgcg cgaagatcgc tcaccgcgag
      661 tccgcgccgc gccacctgcg cgggtggtgt gtcgaccggg aggaggaacg attccgcctc
      721 gctcgcgccg ctctttcggg cgggcatgcc gtggcccgag tagtagacgt agatcgtgct
      781 gtcgcgtcga attgggagct gagcgagcac cgggccgaca ccgccgccgc tctccaccca
      841 gtcctggaag gtcccagacg tcgcgtcctt gatgacgatg atgcggcttt cggggacggc
      901 gagcacgtcg cgggcggcgc ggcgcatctc ctcgccgtcg cggtgcgcaa agccggcttt
      961 cggcacgctg ccgacgtaag tctcgttggc gaagatgagc acgatgtcat tcgggcccgc
     1021 agcagcagga ccggcaagga agaagatcgc ggcaagagcc gcgacaagcg cgcggagcat
     1081 ggcaagcctc ccagacagaa tgccggatcg taacagcgtc taccgatgat tgtcaccggg
     1141 ggtgccgcgc cggatcgccg ataattgatc tcgggcgcgt aacggggacc ggcaccgcga
     1201 gtaaaatgag aggtcggggc gtcggcctaa ccgtcgagcc attctccgat ccaccagaag
     1261 aagccgaata tcggatcgtc gggccgtcgt gcaagcgggc aggatgcggg aagttgcgga
     1321 gcgacatgga gccggcggag aggcttcgtc cgagcttgat caaaacgatg gcgcgctctg
     1381 gagatggtcc acccggacga agcactcgta gcgctcagca gaatttcctc tgctgctttg
     1441 aaatcggcct cggcacccat tcgatccatt tttgacaatc gcaacaaggc tcgattgtag
     1501 cgcgccgcga acagcgtcgg atcgagttcg atggcacgcg acaggtcctg aaatgaggct
     1561 gagagtgctt cactgcggtt cgcatacggg cttccacgtc gccattcttt agcactgagc
     1621 gaagccctcg cgagaagggc tgctaggtgc gtagaatcgg ccttcaaggc ccgatccaag
     1681 tcgttcctcg ccagctcgtc ttggcgggtc ctttcccaag cgaaggcacg gcaaaagtgc
     1741 gcatccgcca gatggcggcc cgtcagcgat ccggtctcga tggtccttgt catttccgaa
     1801 atggtattgg catttcgttg ccagcttgct tccaggatga tgaagccaat gacgacggca
     1861 gcaattgcaa gaaggctggc aaatatgacg ccgaagaact tgagcgaata gcggagaggg
     1921 ctgcctcgct cgaagccctt tggcgggccg cgccgcgcct tcaattcggc atcgataagg
     1981 tgaattctct gctgaatccc atgtgcgaat tgcttgtctt gccagaccca aatggagccc
     2041 atggccggat cgaaccggag cttccggtca cgtgtcaacc gtagggaagc gtagaggccc
     2101 gcaaggcctg gggcgaggca ggcagcgcag aagacccaga atgcgctgcg aacactctgg
     2161 gcgagcagtg gctgaacggc tgtcctccag agatccgaga cgaggtagtg ccaaaggctc
     2221 aagatcactg aggcaacgga caccaggctg aacggaatgg ccaccgcgat gccggccgcc
     2281 gcggcgtaga gatgccacgg gcggatgacg acgactctca acttgtccag ccgtttcggt
     2341 cgtcgcggca ggcgcttgac gacgtccatt gcatctgccg cggtcgcagc tgtgtcgcga
     2401 cttacttcac cgtcggcgat cctttgcagc tcttcgacgt atcgtgcccg ctcatccggc
     2461 gacgaggtgg cggcggattg cacgacgagg ttgacgattg ccaaatgatg gtcggtgaac
     2521 tggtacgacg tccttggtga tgactgttga gacatgacca cccccaagcc ccgcagtaga
     2581 tcatcgagaa gcttctgtcg cagcggccac gtgacatcga atccaaagac agcttgtggg
     2641 cggtgttagg ccggcttcga ccgaccgagt ggccggcctc tcgatcgtcc atgcgagcca
     2701 taatgcaatc cactcacccg taggcgaccg tcgcctccat ggcgatcgtg ccctcgggcg
     2761 tgacggccca gagcttggca cccttcccgt cctcgctcgg gcctccgacg acagagaagg
     2821 gggcgatgtc gaagagcggc gcgcgggcgc gctgggagta gcgcgtgata cgccggccac
     2881 ccgcgttgtc gcgcgcgagg tcgatcaggc actgctgcga gtagggaccg tgcacgacga
     2941 ggcccgggta gccctcgacc gtcatcgcgt aggtgcggtc gtaatggatg cggtgcgggt
     3001 tgaaggtcag cgccgaatag cggaagagcg agaccggatc agcggtgatg gtgcggctcc
     3061 aggggaggcc cgcgggcggc gcgtcgcgct tcggggtcgc gttcttctcg cccgccttca
     3121 cgtcctcgcg gaagaccgtg tggctttcct cgacgatggc gaggccgcgc cgcgtgtaga
     3181 tgcgccgcgc ctgcgtcgtg aagatcaggg gaccggtcga acccttgcgt acgtcggcgc
     3241 cggtgaactc ggtctcgcgc gtgatggcgt cgccgacgcg gaggtcgtcg tggaagtgga
     3301 tgctcgaacc cgcatacatg cggcgcggaa atggcatctt cggcagcacg ccccaggtgg
     3361 tcggcagccc gtcctcggcg agcacggcgg ggggcgtcgc ctgcatgaag tagccgagat
     3421 gccagccggg cgcgatgcgc tcgccggctt tcggcgcggg ctcatagcga ttgaaggtcg
     3481 cgatcatgca gcgtggaggc gacgatgtcg cgatatcctc ctcgatcatc ttcgtgccga
     3541 tgtggcggcg gatggcgtcg atgtcgacgg tcatggctgg ctccggatca ggctttgtcg
     3601 agaacgaagg cggggagcgc gagctcggct gtcggccgct cgatgacgag gcggacaggc
     3661 gcgccgagct tcaggctcgc cgcctcggcg ccccgcagcg gcgcctgcat gcgcacgccc
     3721 tcgggaagat cgacggtcac gagagtgaac ggcacctcgc ccttgaaacc gggatggaac
     3781 gggtgatgtg tgaccgtcca gctgtgcacc agcccgcggc ccttggcctc gctccagcgc
     3841 acctcgaagg agtagcagga ggggcagatc ggccgcgggt agtgccggac cttgccgcag
     3901 ctgccgcagg tctggaggag cagctttccc gcgaggagac catcccagaa cggtttcgat
     3961 tcctgcgacg gctgggggat cggctttgcg atcgtctcag cgctcatgtc agcccctccg
     4021 catgatggcg atcgagccgt cgcccatgtc accgtagccg gtgacgaggc cgacctcggc
     4081 gtccttcacc tgcgccttgc cggcgtcgcc gcgcagctgc cggacgagtt ccacgacgtg
     4141 gttcatgccg accatgtgcg attgcgagag gagcccgcca tgtgtgttga tcggcagctt
     4201 gccgccaagc cggatggcgc cagaggcgac gaacgctccg ccctcgccct tcttnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn gacgcccgcc atggcgaagg ccttcggcgc ggccttggcg atgccgaggc
     4381 gggtgaggtc ggggcgctgg gtgatggcgc tcgggctgtc gggatgcccc tcggccatgc
     4441 ccatcacgta gacggggcgc tgcttcaggt ctcgcgcgag gctcgcggcg ctcaccacaa
     4501 ctgcggcgcc gccatcgctc tcgaggctgc agtcgaggag acggagcggg tcggagatca
     4561 tgcgcgaggc ctggtgatcc tctattgcga tcggcttcat catcatggcg cggccgttga
     4621 ggatcgcgtt cgcgcgcgtc gagactgcga tctcgccgaa ctggcgcgag gtggtgccgt
     4681 acagttccat gtggcggcgg gccatcggcg catagagctg tgccgggccg atggcgccca
     4741 ccggcatctc gtgctcgccg atcaagcgga actggggcat ctcggcgacg cggagcccga
     4801 tgcggccctg cgaggcgccg ttgcggccga tcggcaggag gacgtggcgg cagagaccgg
     4861 ccgaaatcgc gagagccgcg cattggatgg ccgcgaccgc gcttgcgccg cccatcggcg
     4921 tggttgccga gaatcgaagg tcgtcgaggc cgaagttggt gatgaagtcc tcggcgacga
     4981 ccggggaatt cgagtagggg atgatgccgt cgatgtcctt cgggctgaga cccgcgtcgc
     5041 ggatcgcggc gagtgacgct tcgagctgaa gcgcggcgac actcttgcct gagttcctcg
     5101 tataggcggt ctcgccgacg cccgtgacgg cgaccgcttc cttgagcgtc atcgcgtgcc
     5161 tccctgattg atccggaacg ctaacacgcg caagcgccac gatgtaaggg acgcggcggt
     5221 ccttcgcttt gaataccgga cccgccgaaa tcagggagat cgcaggcgcg atcgctggta
     5281 cgcccatcgc gagggagacc gtgcttcgtc agtgtcagcc ccaagggagc cagttgcaaa
     5341 actcttggcg ggctgacggg gtggtgccag ctgcctcgtg gatggcgaca gcggcgagcg
     5401 actttgtatg cgttgccgtc cggcattgtc cgatacgaat gagggcggac gcaagagtgc
     5461 caaggggcgc atggaccatg cacctccgga tgctgccgga ccggctccgc gagcctctca
     5521 gacaaacagg cgcatgagtt ggtcgacggt gagcgcgagc actccaaaca gcagatggta
     5581 catcacattg gcgtggccgc gcacgcgcac gaagcgtccg ccgaacccat ccttgaggcg
     5641 ggcgttgacg ctccacagcc gtgcgctcgc ggtagccgat gccctcagct gtcggcatgc
     5701 agatgttgcg gcgagcaagc gcctcggcgg cggcctctcc cgtgaacccc cggcgcggct
     5761 gcttgggaac gaccccaccc gtccgctctc ctgtgatgat tgcagctgcg ggctggctcg
     5821 tatggtacaa ggaggggctc ggcgccgcct tcatcacttg ggtcgcggca ctcggcctca
     5881 acgcggcatg gtcgtggccc ttctttggcc gccaatggaa cggccgcgcc cttgtcgaca
     5941 ttctcgcctt gctggctgcc atcgcaatct tcattgcgat gacctggact cccgcccgga
     6001 cggcggcaat gctccttgtc ccgtatctgc ttcgggtcgc gttcgcctcc ggccagaacc
     6061 tcgagatctg gcaactgaac cggtgacgtt cgagccgttc cccgaccaat ccaaatcgcc
     6121 tgccacgtcg ttcaaggacc aagggcggcg agcgggagat cgcaatggag ggtggcaagg
     6181 agggcggcaa acgcattgcc gtgatcggca gcggcatctc cggcctctcg gctgcgtggc
     6241 tgctcgcaaa gcgtcatcat gtgtcgctca tcgaggccga catgcggccc ggcgggcaca
     6301 gcaacacggt cgatgcctgc ctcggacgct gcacgatccc cgtcgacacc ggcttcatcg
     6361 tctacaatac ggcgagctat ccgaacctta ttgcgctgtt cgagcacctc ggcgtcccaa
     6421 cggtcgcgac cgacatgagc ttcgccgtct cgctcgatgg cggacgctac gagtactcgg
     6481 gatcaagtct cggctcgctc ttcgggcagc gctcaaacct tttccggcca tcgcactggc
     6541 gcatgcttgc cgatacgatc cgcttctttc gcgaggctca gggcgcggcc gcactgaacg
     6601 cgagcccggc tgtgacactc ggcgccttcc tcgccgggcg cggctactcc gaggccttca
     6661 tcaagcggca tatcctgccg atggccgctg ccatctggtc cacgccgtcg cgcgctgtgc
     6721 tcgacttccc gctcgcagca ttcgtgcgct tcttcgcgaa ccacggcctg ctgcaagtcg
     6781 cgaaccgtcc ggagtggcgc acggtcgtcg gcggtagccg ggagtacgtg acgaggttgc
     6841 tcgctgattt cgacggcgag gtgctgctcg gcgccggcgc acgacgcatc acgcggcacg
     6901 gcgacggtgt cacggtcgag acggctcgtg gcgaacagcc ttatgatggc tgcgtgatcg
     6961 cgacgcatgc cgacgacgca ctcagcctgc ttgccgatcc gagcaaggac gagcgcgatc
     7021 tactcggggc ctttcgctac atcgccaacc gcgccgtcct gcataccgac cgttcgcaga
     7081 tgccgcgacg caccggcctc tggtcgagct ggaactatct cggttccggg gcgggcctgc
     7141 atggcaacct cgctgtgacc tactggatga acaagctgca gccgctcggc ggcagcgcgc
     7201 cggacctctt cgnnnnnnnn nncccgccgc cgacgttcga gccgtcttcg atcgtctccg
     7261 cgttcgacta cagccatccg atgttcgatg cgcgcgcgat gcaggcccag cgtcggctct
     7321 gggatctcca gggctgccga agcacctggt ttgccggcag ctatttcggc ttcggcttcc
     7381 acgaggacgg aattcaagcc gggctcgctg cggccgaggc catcggcggt gtgcgacggc
     7441 cgtggtccgt tgccgaggag tcgggccgca tccatatcac cccacgtgcg ccgcatgagg
     7501 acgcgtccgc gtgtgcccga tatcgcgagg ccgccgaatg accgcgtccg gctctgccat
     7561 cttcgagggc acggtcgtgc acaagcggct ttcgccgaag ccgcacgcct tcgcctaccg
     7621 cgtcttcacg ctctgcctcg acctcgacga gatcgcgtcg ctcgaccgcg agctgcgcct
     7681 gttctcggtg aaccggcgga atgtcgtgag cttctgggac agcgatctcg ggcccggcgg
     7741 tccggaagcg gcggccgaga aggcccgcgc cctcctcgat cattgcggcc tcggcgtgta
     7801 tggcgcacgc atcgaactcg tctgctatcc gcgcctcctc ggctacgtct tcaatccgct
     7861 gagcgtctac ttctgccgca acagtgatgg ccgcctcggc gccgtggtgt acgaggtgag
     7921 caacaccttc agcgagcgca agagctacgt catccccatc gagggagaga gcggcatcat
     7981 ctcgcagcaa tgcgtgaagg atatgtacgt gtcgcccttc acgaaggcgg cgggcagcta
     8041 cggcttccat tgcgtggcgc cgtcagagcg ggtcgtgatc ggagtgaact tccgcgaagc
     8101 cggtcgtccc gtgctcaaga cgcacttcgc cggtgtgcgc cgaccgctct ccgatcgcgc
     8161 gctcctgtcc gctgtcacgc gccacccgct catgaccatg aaggtcatgg ccgccatcca
     8221 cttcgaggcg gcaaggcttt gggccaaggg cgtcccggtc gtcacgcggc acgcctcgcc
     8281 ggcctattct tacacccgcg tcaacagctc ccgagggacg cccgcccatg tctgatgagc
     8341 cttcagtcgg aagacccgcc gcagcgccag ccgccacggc tccgctcggc ggtctggcgt
     8401 ccgtcggcac gctgcccttg cgactgctcg acggactctt gcgtcggcgg ggaatcggcc
     8461 ggctccgtgt tcgcctgcca ggcggcgccg aaggggtgat cggcgaagcg ggttcgcccg
     8521 tcgaggcgag cctggaggtc cgcgcctacg ctgccgtctg gaaagtctgg cgtcgcggcg
     8581 cgctgggttt cgcggaatcg tacatggacg gcgatttcga aaccgccgac ctcaggcgat
     8641 tgttcgagct ctatctcgcg aacgagccgg cactcacacg tgcttttccc cgcctcaacc
     8701 tgacgcgcgg gctcgaccgc cgcttccacg cgagccggca gaacacgtac tcaggcagcc
     8761 ggcgaaatat cgccgagcac tacgatctcg gaaatgcctt ctaccggctc tggctcgacc
     8821 cgagcatgct ctactcgagc ggtatctaca cgagcgacac tgcgtccctc gaggaggcgc
     8881 aggcggagaa aatcggccgc atcctcgccg ccctcgagct gttgccggag caatccgtgc
     8941 tggaggtcgg atgcggctgg ggtgcgctcg cggcggcaat ggccgagcgg ggtgcccgcg
     9001 tgcgcgccat cacgatctcg gcggagcagc tcgccgaagc ccgcgcccgt attggccggg
     9061 ccggactttc agaagtcgtc gaaacggtct tcgaggacta tcgcgatacg gttggcggtt
     9121 tcgatcgcct cgtctccatc gagatgatcg aggccgtcgg agaggagaac tggccacttt
     9181 tcttcggcac gatcgcagac aggctcaatc cgggcggaac ggccgtcatc caggcaatca
     9241 cgatccgcga agatgcgttc gcctcctatc gcgcaaatcc ggatttcatc cagcgctaca
     9301 tctttcccgg cggaatgctc ccgaccgtca aattgatgca ccaacgcgcc agcgaggcgg
     9361 ggctctcctt cgagatcgtc gagcgcttcg ggcactccta cgcgcttacg ctcgcggaat
     9421 ggcgccggcg cttcgaacac gcttggccgc agattgccgc gctcggattc gacgagcgtt
     9481 tccgccgcat gtggcgctat tacctgacct actgcgaaat agggttcgaa cgagggctga
     9541 tcgatgtcgg cctctaccgg cttaggaaag cgtgagagcc ggaaacgcgc cgcgaaaatt
     9601 gtccaactga tcaacgcaac gagatctcgt catgccacag gatatggcgc cggccagcat
     9661 tcagagagtg ccccggtttt tcgacctcac gtccttcctg cacggacgaa cgcgcgcctg
     9721 gggcgttttc gaggatcgct tcggcaacgt gagacgtcgg ctcaccgtcg acatgctcgg
     9781 ccgctgggag ggtggcgagt tcgtgctcga agagcagttc gtctacgacg gcgtctcgtc
     9841 cgagcagcgc acctggcgcg tcgtccccga ggcgaatgga cgcttccgcg ccgcatgcag
     9901 tgattgcgtc ggggaggcga tcgggcagtg cgcgcaggac agcgtgcgga tgtcgtaccg
     9961 gttccgcttg aagctcgacg cacgcgatct cgtggtcaca ctcgacgacc ggctctacca
    10021 gatcggcccg gaccacgcga tcaaccgcgc gcgcatgagt aagtggggcg tcaagcttgg
    10081 cgagctttct ctcttattcg agcgcattcc tgacaggaag tgatcctcaa agccgacccg
    10141 cgagacgcag aaagagcgcg ttcggcagga tgcgcaaaag cttcataatc gtggcgagct
    10201 gccatgggaa gacgatctcg aaccggccgc gttcgagccc cttcaggatg cgctctgccg
    10261 catcgtcggc cttgatgatg aagggcatcg gaaaggtgtt gattgaggtc atcggcgttt
    10321 cgacgaagcc gggattgacg aggctcacct taatgccgtg gcgcggcagc tccagccgca
    10381 acacctccgc gagcgagatg acggccgcct tggtcggcgc gtaggcaacg gcattgggca
    10441 agccgcgata gccagccatc gatgcgacga gtgcgatgtg acccttgccc gctttcgtca
    10501 ttgccgggat gagaagctcg agcgcattgg caatggcgac gtagttgacg ttcatggact
    10561 ctgcgatccg cgtcgcgtcg agggcactgg ccggcatcgg ctgccagacg ccggcattca
    10621 acacggcgag gtcgatcgca cccagctcat cggagatgcg ggcatgcgcc gccgccgtcc
    10681 tgctgcgatc ggtgacatcg agcgggaccg gcagcaacga cggatgcagc gccgcgagct
    10741 cagcgagccg ggcttcactt cgcgccgatg cagcgacgcg cacgcctcgt ccagcgagct
    10801 tcagcgtgag ctctcggccg atgcctgtac tggcgccggt gatccatgcg gtgcgccagg
    10861 ggctcggcat cacgacctcg ccgcagtcag agaccggtcc acggctgcgt caagcggccg
    10921 atcagcgact tgaagcgcgc gggccgctcg ctcgcgatga ggcagatgca ttcggcctcc
    10981 ttgccgacaa ttggctgatg atcgacgccg ctgtcgacat cttgcacatc accacgcccg
    11041 taggagcccg tcacgtcgct gtaggaccct tcgagaacga gtgtcagctc gctgccgccg
    11101 tgtccgtgct ccggcatcgc ccggcccggt gcgatgcgca gcagtcgaag gtccccctct
    11161 gcgggctggc acaacggcaa tttgtgatgc catacgcccg gaccaaggcg gcgccatggg
    11221 atctcgtccc acgccaagcc gtaggcgaga gccagcggcg cggggaggcg ttcgtcgatc
    11281 ggttgcacgg gcttgccgac gaagctcgcc gtgtccggcg gccgccgccg tggcgcaagc
    11341 ggcgtctccg acggcgaggc cgtgtcggcg aacatgagag cagcgccgag gcgctcgagg
    11401 tcagcgacct cggaccggca gcgcgggcac atggcgaggt gcgcggcgac cacggccgcg
    11461 agcggctctg agagcgttgc cgcggcgtag ctcacaagcg ttgcctcatc gggatgatgg
    11521 gtaatcggga tcatcatcgg atatcctcga gtgcggctcg caatttctgg taggcaagtc
    11581 ggatacgtga tttcaccgtc cccaacggca aggagagacg atcggctatc tcgctgtggg
    11641 caaggccatc cacgaaggcg agggtcacga cctcgacctg ctcgggcggc agctccttca
    11701 agatggcttg cacgcggagc tgacgttgcc gctcggcgac gaggtccggg cggtcgcggt
    11761 acgacagcac gcgcgcg
//
LOCUS       DAORNH010000128        11748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4131_length_11748_cov_2.819892, whole genome shotgun sequence.
ACCESSION   DAORNH010000128 DAORNH010000000
VERSION     DAORNH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_11748_cov_2.819892"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PK264_13520"
     CDS             complement(<1..674)
                     /locus_tag="PK264_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HRD76930.1"
                     /translation="MSWRGGGLALPLFAFLLLPVHGSAQGVDTVKKQLEETKRKLEEA
                     KRRQDATAAGVADVARQREALNKQLLETAGLIQASEARLSELDNRIGGLEELERQLRG
                     SLAQRHDQIARILAVMQRMGHNPPPVMITRREDALRMVRSAMLLAATFPETQKHALQL
                     KKDIDELLKVVGDIKAERDKARSESERLAASQLKLAALMEEKRLTLAEQQEQLERIRR
                     EAEAIRS"
     gene            complement(837..1793)
                     /gene="gshB"
                     /locus_tag="PK264_13525"
     CDS             complement(837..1793)
                     /gene="gshB"
                     /locus_tag="PK264_13525"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HRD76931.1"
                     /translation="MSLKVACQMDPIDRIDIRGDSTFAMLFEAQKRGHELFVYTPDKL
                     ALDGPKLLARGTTVVLRDKIGDHVTVSNPRAEDLTSWDVVLLRQDPPFDMGYITTTHL
                     LERIHPKTLVVNDPAHVRNAPEKIFVLNFPELMPPTLVTRNPEEIKDFRARYKDIIIK
                     PLYGNGGAAVFRLKPEDTNLNSLIEIFHILNREPVMIQEYRPEVRAGDKRIILVDGEA
                     VGAINRVPAADETRSNMHVGGVARPTELTPRDLEICRRIGPELKARGMIFVGIDVIGP
                     FLTEINVTSPTGIRQVKAFGGADIAALIWDAIERKTSAGRRA"
     gene            1974..>4368
                     /locus_tag="PK264_13530"
     CDS             1974..>4368
                     /locus_tag="PK264_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HRD76932.1"
                     /translation="MTDRARRAFRTLLAAVSALIVVATAVVAQSPPTPPGPAAPPAAQ
                     PAPAPIPSKPQLPSEVADTIDRLAAVMDSVEKSVAKLTDAEQDLGRLRDDVERIITQS
                     TETADKLRPRLSALRSQIERLGPLPGKDAPAEAAAITAERARLTAEATNLDGAIKTLE
                     LTWVRARQMIDKITDLRLTLFTKSLMERMSSPLLPSLWRSVMNDFPPLSWLIGYVARD
                     WWSWAQRRIVPLSGVLGGAALLYLALRWLAAWLVRKWDRAADAPAFTFFERAADAAWV
                     AILRALPGMAAALAIYVGLDLLDLLYYPTDRIGGAVLKAVLLFVGISALIRALFAPRS
                     PHRRLLPIDDQAASRVCRLLQTMAGVTALDTSLTAVGRALYAPLSISVAQSFLMSMLF
                     AALLIGLLLTPFVPRGSPPDVAQTWPKSHPRWLKGPLWLVALAIIAASVLGYLALGRF
                     VTQQLMVTGVLIVVATLLYLAIRAFTRESETAMPVNAVLEGRFGLDPPRRQQIVKLVE
                     LALTLTLVILALPILLLQWGFSGADIRDWLKSAIFGFEIGQFKISLARILIGVVLFTA
                     LLFATRLFQRWMRESVLVTPRVDPGIAHSIDTAIGYVGIAVSAIIAVSYAGFDITSLA
                     IVAGALSVGIGFGLQSIVNNFVSGLILLIERPIKVGAWIVVGDQQGNVRRISVSSTEI
                     ETFDRASLIVPNSELITGRVLNWTHRNQLGRLIVKITVGSTADPEKVLAVLRDCAGKH
                     ARVLGTPEPRASFDNFGANGLDFSVRMVLDDVNRSLEVQTDLRTAILKRFREEGIEIP
                     "
     assembly_gap    4369..4538
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4567..4641
                     /locus_tag="PK264_13535"
     tRNA            4567..4641
                     /locus_tag="PK264_13535"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4599..4601,aa:Val,seq:gac)
     gene            4715..4927
                     /locus_tag="PK264_13540"
     CDS             4715..4927
                     /locus_tag="PK264_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HRD76933.1"
                     /translation="MTAEIIRFRRVRKEKARQEREAEAALNRARFGRSREERERDEAR
                     AELERRRHEGNRLRHKPSNDDEPTSA"
     gene            4947..5159
                     /locus_tag="PK264_13545"
     CDS             4947..5159
                     /locus_tag="PK264_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HRD76934.1"
                     /translation="MRPVKHSFTIRGHRTSVSLEAPFWDALRAAATRDGRSLSALVSS
                     IDAERGCANLSSAIRVWLLRDLQVRL"
     gene            complement(5163..5516)
                     /locus_tag="PK264_13550"
     CDS             complement(5163..5516)
                     /locus_tag="PK264_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76935.1"
                     /translation="MPIRPRSRFCLAASGLAVLCHLLIAWLAPASAQGEACSTLACPK
                     CEQLCRGYCEIENQKCAAKGRRGCPGAYRACTRGCTAELCRQCVPIQHVEKDKTFHAG
                     TTNLCQTPGYTSGKK"
     gene            5668..6660
                     /locus_tag="PK264_13555"
     CDS             5668..6660
                     /locus_tag="PK264_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HRD76936.1"
                     /translation="MRAVAMRGHGGADVLAVIDAPVPEPGPGEALVKVSFAGINFIDI
                     YQRSGTYSAASKPTPQAPIILGLEGAGEIVKVGTGVSDLKPGERVAWSGSPGSYAEFA
                     RVPAWKLVPVPADVPLDIACAVQLQGGTAHYLATSTFPLKSGDVALVHAAAGGVGQLL
                     VQLAKVCGATVIATVGSDAKAELAKARGADHVIVYTREDFLARVLEITANQGCHVVYD
                     SVGKATLAKSIAATRRRGLVISYGNASGVPDAPSPMALADAGSLFFTRPRLGDYMQDA
                     TEIRSRIGDLLQGVRSGALKVTIDRIWPLAEARQAHETLEARGSHGKLLLKAIG"
     gene            6721..7614
                     /locus_tag="PK264_13560"
     CDS             6721..7614
                     /locus_tag="PK264_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRD76937.1"
                     /translation="MEAFHGALTRLIVPGRSTVADIGTGSGVLAMLAARLGARKIYAY
                     EMAEIGAVAERLVRHNKFRSIELIPGRSTDIIDPPRVDIVVSETLGNYALEENIIETM
                     NDAVARHLAPGGTIIPARLEQFVAPVVAPRLRDELAAWDRVGLGLDLGPATAFSLNNA
                     YVRTLVPDELLDRGKSAQVWDRIDLSKKNRLSRKGRASWLMEAQATIHGLAVWWCATL
                     VDPIALSTGPFDPATHWEQLFFPALDPISLSRGETLAIELSSRSSDEGGTDLAWTLVR
                     VNAKGREAERQALSLDQGFIP"
     gene            7811..>8363
                     /locus_tag="PK264_13565"
     CDS             7811..>8363
                     /locus_tag="PK264_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A oxidase"
                     /protein_id="HRD76938.1"
                     /translation="MQRIERLGRIAAIALLAGSMADLWINETSGPTDPPAEEFRAASA
                     ALAGHDLTDRETTVSGYVGAPFYYRSDVRMSRNDDTDVVLKRLGWDGDALYFPIDGGV
                     RSVSWNGSFGFMIDFLHNKAIARLGRGAHGRRLANPIVEEVEATGRLKGAPAPARLKL
                     TDVFERLEFTHGHNVLLLNGMMRL"
     assembly_gap    8364..8375
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8376..8771
                     /locus_tag="PK264_13570"
     CDS             <8376..8771
                     /locus_tag="PK264_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A oxidase"
                     /protein_id="HRD76939.1"
                     /translation="PRVRPYIGIGGGIAVPHVEVWFPGANAVERTNEYQYAGPAAQAV
                     AGFEISAGRVTYFVEYKFTYAWISSTLTADQSWKNFNMPGDLFRQFRRWLAGSSPKIG
                     TISTTLGAHQIVAGAGYTWRAPKADPSSR"
     gene            9289..9606
                     /locus_tag="PK264_13575"
     CDS             9289..9606
                     /locus_tag="PK264_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HRD76940.1"
                     /translation="MKDSQLEKMSVAELRDLVTRANAIISNKAQQEKAQLRERWLREA
                     KEAGVPVEIRFTDSRKGVRKGSGADVKFQHPDDPSLTWSGRGRKPKWLSEAGGNIERF
                     RIA"
     gene            9903..10652
                     /locus_tag="PK264_13580"
     CDS             9903..10652
                     /locus_tag="PK264_13580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRD76941.1"
                     /translation="MARHARSASSHGTIDVAGIPTPVEIRRHPSARRLTLRVSRTDRS
                     VVLTVPNGSDLGEAGRFAGRHADWVRKRLESVPSSVAFVDGAVIPVRGIAHSLRFSGE
                     TRAEGVVVANPTDQGVPAALLVSGRVEHAPRRLLEWLMREACRDLDASVLHHAGRLGL
                     RPRRITVRDQRSRWGSCSSTGRLAFSWRLILAPTFVLDYVAAHEVAHLAEMNHGPRFW
                     SLVEQTCPATQQAKAWLSAHGTTLHGYGPPS"
     gene            10783..10858
                     /locus_tag="PK264_13585"
     tRNA            10783..10858
                     /locus_tag="PK264_13585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10816..10818,aa:Lys,seq:ctt)
     gene            complement(11082..11175)
                     /locus_tag="PK264_13590"
     tRNA            complement(11082..11175)
                     /locus_tag="PK264_13590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11139..11141),aa:Ser,seq:gct)
     gene            complement(11286..>11748)
                     /locus_tag="PK264_13595"
     CDS             complement(11286..>11748)
                     /locus_tag="PK264_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRD76942.1"
                     /translation="IAKTDDYGRIMLKALADRLAEALAERMHERVRRELWGYQPTEKL
                     SNEELIAEKYTGIRPAPGYPAQPDHTEKATLFRLLDAEAQAGIELTENYAMWPGASVS
                     GLYFSHADSHYFGVGKVERDQVEDYAGRKGWTLAEAERWLAPVLNYDTKAS"
BASE COUNT         1935 a         3857 c         3678 g         2096 t
ORIGIN      
        1 gagcggatcg cctccgcctc gcgccggatg cgctcgagct gctcctgctg ctcggcgagc
       61 gtcaaccgct tctcctccat gagcgcggcg agcttcagct gcgaggcggc aaggcgctcg
      121 ctttccgagc gcgccttgtc gcgctctgcc ttgatgtcgc cgacgacctt aaggagttcg
      181 tcgatgtcct tcttgagctg aagcgcgtgc ttttgcgtct ctgggaatgt cgccgccagc
      241 agcatggcgc tgcggaccat gcggagcgcg tcctctcggc gcgtgatcat gaccggcggc
      301 gggttgtggc ccatgcgctg catgacggcg aggatgcggg cgatctggtc gtgacgttgg
      361 gcaagcgagc cgcgcaactg ccgctcgagc tcctcgaggc cgccgatgcg gttgtcgagc
      421 tcgctcaagc gagcctcgct cgcctggatg agtccggcgg tctcgaggag ctgcttgttc
      481 aatgcctcgc gctggcgggc cacatccgcg acgcccgcgg ccgtcgcatc ctggcggcgc
      541 ttggcctctt cgagcttgcg cttggtctcc tcgagctgct tcttgacggt atcgacacct
      601 tgcgcggagc cgtgcacagg cagcagcagg aacgcaaaca gtggcagggc aaggccgccg
      661 ccgcgccacg acatcacgcc tgcattcgat cgcccgcttg acggcaaagc tctgtcccac
      721 ggaaatcctg cgcccgacgc acgtccgggc tgagcccggt tagtatgtcc gcattgagtg
      781 cacaataggg cccttggcga acggccaagg tcagcgtgca aatgtggccg gccgcctcaa
      841 gcccttcgcc cggccgacgt cttccgctca atggcatccc agatcagcgc tgcgatatcg
      901 gcgccgccga aagccttcac ttgccggatt ccggtcggcg aggtgacatt gatctcggtc
      961 aggaaaggtc cgatgacatc gatcccgacg aagatcatgc cgcgcgcctt gagctccggc
     1021 ccaatccgcc tgcagatctc gaggtcgcgc ggggtgagct cggtcggacg tgcgacgccg
     1081 ccgacgtgca tgttggagcg cgtctcgtcg gccgccggca cgcgattgat cgcgccgacc
     1141 gcctcgccgt cgaccaggat gatgcgcttg tcgccagcac gcacctcggg gcgatactcc
     1201 tggatcatca ccggctcgcg attgaggatg tggaaaatct cgatcagcga gttgagattg
     1261 gtgtcctctg gcttcagccg gaacacggcc gcgccgccat tcccgtagag cggcttgatg
     1321 atgatgtcct tgtagcgggc ccggaagtcc ttgatctcct cgggattacg cgtgacgagc
     1381 gtcggcggca tcagctcggg aaagttgagg acgaagatct tctccggcgc attgcggacg
     1441 tgcgccggat cgttgacgac gagcgtcttc gggtgaatgc gttcgaggag atgtgtcgtt
     1501 gtgatgtagc ccatgtcgaa gggcggatcc tgacgcagca gcacgacatc ccagctcgtg
     1561 aggtcctcag cgcgcgggtt cgacaccgtg acgtgatcgc cgatcttgtc gcgcaaaacg
     1621 acggtcgtgc cgcgtgccag cagcttcgga ccgtcgagcg cgagcttgtc gggcgtgtaa
     1681 acgaagagct catgcccgcg cttttgcgcc tcgaagagca tggcaaacgt cgaatccccg
     1741 cgaatgtcga tgcggtcgat cgggtccatc tggcaggcga ccttgaggct catgggctct
     1801 ccgacgtctc gtgtccgcga tgatgtcgca tgcaaatgcg cggtcgtgcg tgctgtcaac
     1861 gttttccttt gggagaagag gccggcgata ccggatcgcg ccgttaaccg ctcttgatcg
     1921 gccggatttt tccgggcaca tggctcaccg cccaagcgat gagcgaccag gacatgaccg
     1981 atagagcccg cagagcattc cgaacgctgc tcgccgccgt gagcgcgctc atcgttgtgg
     2041 caacggctgt cgttgcccag tcgcccccga cgccgcccgg ccctgccgcg ccgcctgcag
     2101 cccagcccgc gccagcaccc ataccgagca agccgcagct gccgagcgag gtcgccgata
     2161 ccatcgatcg cctcgccgcc gtgatggaca gtgtcgagaa gtcggtcgcc aagctcacgg
     2221 atgccgagca ggatctcggc cgtctccgcg acgatgtcga gcgcatcatc acgcaatcga
     2281 cggaaacggc tgacaagctg cggccgcggc tctcggcgct cagaagccag atcgagcggt
     2341 tgggaccgct gccaggcaag gacgcgcccg ccgaggctgc cgcaatcacc gcagaacggg
     2401 cccgtctgac ggccgaggcg accaacctcg acggcgccat caagacgctc gaactcacct
     2461 gggtgcgcgc gcgccagatg atcgacaaga tcaccgatct gcggctcacc ttgttcacga
     2521 agagcctcat ggaacggatg tcgagcccgc tgctgccctc gctctggcgc agcgtgatga
     2581 acgacttccc gccgctctcc tggctcatcg gctacgtcgc tcgcgactgg tggtcatggg
     2641 cgcagcgtcg catcgtgccg ctgagcggcg tccttggcgg cgccgccctc ctctacctgg
     2701 cgctcaggtg gctggccgca tggctcgtcc gcaagtggga tcgggccgcc gacgctccgg
     2761 ccttcacctt tttcgagcga gcggccgatg ccgcctgggt cgccatactg cgcgcgctgc
     2821 ccggcatggc cgcggcactc gccatttacg tcggtctcga tctgctcgat ctcctctact
     2881 atccgaccga ccggattgga ggcgcggtgc tcaaggcggt gctgctgttc gtcggcatct
     2941 cggccttgat ccgtgccctg tttgcgcccc gttcgccgca tcgccggctg ctgccgatcg
     3001 acgatcaggc agcgagtcgt gtctgtcgcc tgctgcagac catggccggc gtgacggcgc
     3061 tcgatacgtc actcaccgcc gtgggccgcg ccctctatgc gccgctctcg ataagtgtcg
     3121 cccagtcctt cttgatgtcg atgctgttcg cggccctgct gatcgggctc ttgctgacgc
     3181 cgttcgtgcc gcgcgggtcg ccccccgatg tcgctcagac ctggccgaag tcccatccgc
     3241 gttggctcaa ggggccgctc tggctggttg ccttggcgat catcgcggcc tccgtgctcg
     3301 gctatctggc gctcggccgc ttcgtcacgc agcagttgat ggtgaccggc gtgctgatcg
     3361 tcgtcgccac gctcctctac ctcgccattc gcgccttcac acgcgaaagc gagacggcga
     3421 tgccggtcaa tgcggtgctc gaggggcgat tcggcctcga tccgccgcgc cgccagcaga
     3481 tcgtcaagct cgtcgagctc gcgttgacgc tgacgctcgt catcctggcg ctgccgatcc
     3541 tgctgctgca gtggggcttc tccggcgccg acatccgaga ctggctgaag tcggcgatct
     3601 tcggcttcga gatcggccag ttcaagattt cgctcgcccg catcctgatc ggcgtcgtgc
     3661 tgttcacggc actgctgttc gcaacccgcc ttttccagcg ctggatgcgc gaaagcgtgc
     3721 tggtgacgcc gcgcgtcgat cccggcatcg cccattccat cgacacggcc atcggctatg
     3781 tcggcatcgc ggtgtccgcc atcattgcag tctcctatgc cggcttcgac atcacgagcc
     3841 ttgccatcgt cgccggcgca ctctcggtcg gcatcggctt cggcctgcag tcgatcgtca
     3901 acaatttcgt ctcgggcctc atcctgctca tcgagcggcc aatcaaggtc ggcgcctgga
     3961 tcgtggtcgg cgatcagcag ggcaacgtgc ggcgcatctc ggtgagctcg acggagatcg
     4021 agacgttcga tcgcgcgagc ctcatcgttc cgaactcgga gctgatcacc ggccgcgtgc
     4081 tcaactggac gcaccgcaac cagctcggcc gcctcatcgt caaaatcacc gtcggcagca
     4141 ccgcagaccc cgagaaggtc ctcgccgtgc tgcgcgactg cgccggcaag catgcccgcg
     4201 ttctcggcac acccgagccc cgcgcctcgt tcgacaattt cggcgccaat ggcctcgatt
     4261 tctcggtccg catggtcctc gacgacgtca accgctcgct cgaggtacag accgatctcc
     4321 gtaccgcgat cctgaagcgc ttccgcgagg aaggcatcga gattccgann nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gatacatccg gccgggtctg
     4561 gatgacgggt gcgtagctca gcggtagagc actaccttga catggtaggg gtcccaggtt
     4621 cgatccctgg cgcacccacc agacgcctca gctcatgacg tcgggcgcac tgtctcgcgc
     4681 gacgtccgca atgatcactt gtgaggagcc gaccgtgact gccgagatca tacgtttccg
     4741 gcgcgtccgc aaggagaagg cccggcagga acgcgaggcc gaggccgcgc tcaatcgcgc
     4801 ccgcttcggc cgcagtcgcg aggagcgcga gcgcgatgag gcgcgcgcag agctggagcg
     4861 gcgccggcac gagggcaatc gcctccggca taagccatcg aacgacgatg agccgacctc
     4921 ggcctgagac gccgccgctc ggcgatatgc gtcccgtcaa gcattcgttc acgatccgcg
     4981 gccacaggac ctccgtatcg ctcgaggcgc cattttggga tgccctcagg gcggcggcga
     5041 cacgggacgg ccgctcgctg tcggcgctcg tctcttcgat cgacgccgaa cgcggctgcg
     5101 caaacctgtc gagcgcgatc cgcgtctggc tgctccgcga tctgcaagtg cgactttgaa
     5161 gcctattttt tgccgctcgt gtagcccggg gtctggcaga gattcgtcgt gcctgcgtgg
     5221 aaggtcttgt ccttctcgac gtgctgaatc ggcacgcact gccggcagag ctcggccgtg
     5281 cagccgcgcg tgcaggcgcg ataggccccc gggcagccgc gccgaccctt cgctgcacac
     5341 ttctggttct cgatctcgca gtagccccgg cagagctgct cgcacttggg gcacgcaagc
     5401 gtcgagcagg cttcgccctg ggcacttgca ggcgccagcc aggcgatgag caggtggcag
     5461 aggactgcga gcccggaggc agcgaggcag aagcgagagc gaggacggat tggcatgtcg
     5521 cgggtcctta tgcgagggag cgctgaggac cgtgccaagg ccccgtcttg tcggccagac
     5581 gcacgaaggg cgtcccggcg acgcactata ccgccggctc ggatcgtcgt aagttgttca
     5641 aagggtgggt caaccgggga tgtcagtatg cgtgccgttg cgatgcgggg ccatggcggc
     5701 gccgacgtgc ttgccgtgat cgatgcgccc gtcccggagc ccggtcccgg cgaggccctc
     5761 gtcaaggtct ccttcgccgg catcaatttc atcgacatct atcagcgcag cggcacctat
     5821 tccgccgcat ccaagcccac accgcaagca ccgatcatcc tcggtctcga gggcgccggc
     5881 gagatcgtca aggtcggcac aggtgtgagc gacctaaagc ccggcgagcg cgtcgcatgg
     5941 agcggctccc ccggcagcta cgccgagttc gcgcgtgttc cggcctggaa gctcgtgccg
     6001 gttccggctg atgtgccgct cgacatcgcc tgcgccgtac aattgcaggg cggcacggca
     6061 cactatctcg ccacgtccac ctttccgttg aagtccggtg acgtcgcgct cgtccatgcc
     6121 gcagccggcg gcgtgggcca gctcctcgtg cagctcgcca aggtctgcgg cgcaacggtc
     6181 atcgccacgg tcggcagcga tgccaaggcc gagctcgcca aggcgcgtgg cgccgaccac
     6241 gtgatcgtct acacacgcga ggatttcctg gcccgcgtgc tcgagatcac ggcgaaccag
     6301 ggctgccacg tcgtctatga ctcggtcggc aaagcgaccc tcgccaagag catcgcggca
     6361 acgcgccgcc gcggcctggt catcagctac ggcaatgcct ccggagttcc ggacgctccc
     6421 tcgcccatgg cgctcgccga cgcgggttcg ctgttcttca cgcggccacg cctcggcgac
     6481 tacatgcagg acgccaccga gatccgctcc cgcatcggcg atctgctgca gggcgtgagg
     6541 agcggtgcgc tcaaggtcac catcgaccgg atctggccgc tcgccgaggc gcggcaggca
     6601 cacgagacgc tcgaagccag gggaagccat ggcaagctgt tgctcaaggc gatcggctga
     6661 gagcgacgca gcccgatgcg gatcgagtat caccggactc tcatcgctga ccgcgtgcgg
     6721 atggaggcat ttcatggcgc cctcacgcgc ctgatcgttc cggggcggtc cacggttgcc
     6781 gacatcggca caggcagcgg cgtgctggcc atgctcgcgg cccggctcgg cgcccgcaag
     6841 atctacgcct atgagatggc ggagatcggc gccgtggccg aacggctcgt caggcacaac
     6901 aagttcaggt cgatcgagct gattccgggg cggtcgacgg acatcatcga cccgccgcgc
     6961 gtcgacatcg tcgtgtccga gacgctcggc aactatgccc tcgaggaaaa catcatcgag
     7021 acgatgaacg atgccgtggc ccgccatctc gctcctggcg gcacgatcat tccggcacgt
     7081 ctcgagcagt tcgtcgcccc ggtcgtcgcg ccgcggctcc gagacgagct cgccgcctgg
     7141 gatcgcgtcg ggctcggcct cgatctcggt ccggcgacgg ccttcagcct caacaatgcc
     7201 tatgtgcgca cgctcgtgcc agacgagctt ctcgaccgcg gaaagagcgc tcaggtctgg
     7261 gaccgcatcg atctttcgaa gaagaacagg ctttcgagga agggccgcgc gagctggttg
     7321 atggaagcgc aggcaacgat ccacggcctc gccgtctggt ggtgcgccac cctcgtcgac
     7381 ccgattgcac tgtcgaccgg accgttcgat cctgcaacgc actgggagca gctgtttttt
     7441 cccgcgctcg atccgatctc gctcagtcgc ggcgagacgc tggcgatcga gctctcgtca
     7501 cgctcatcgg acgaaggcgg gacggacctc gcctggacgc tcgtgcgcgt caatgccaag
     7561 ggccgcgagg ccgagcgcca ggcgttgagc ctcgaccagg gcttcattcc ttgacctgcc
     7621 tggcctgaac aaccagacac ccctccggcg cgagccggat ttgatcacgg tcattgtatg
     7681 agcgcagcgt gaccatgcgt gctgcggtaa gcacgacttc ccctcgtggg accgacctgg
     7741 gaaaccactc gacatgtcga caacgcagat ctcgccggac cgagcagcga tccaacggcc
     7801 ggcgatccac atgcaacgca tcgagaggct tgggcgcatc gcggcgattg cgctgcttgc
     7861 cggaagcatg gccgacctat ggatcaacga gacttcaggt ccgacggacc cgcccgctga
     7921 agagtttcga gccgcctcag cggcactcgc cggccacgac ctcactgatc gcgagacgac
     7981 cgtttccggc tatgtcgggg ctccattcta ctaccgcagt gacgtgcgca tgtcgcgcaa
     8041 tgacgacacc gatgtcgttc tgaagcgcct cggctgggat ggtgacgctc tctatttccc
     8101 gatcgacggc ggcgtgcgca gcgtgagctg gaatggctca ttcggcttca tgatcgattt
     8161 tctccacaat aaggcgatcg cccggctcgg tcgtggtgct catggccgcc ggctcgccaa
     8221 tcccatcgta gaggaggtgg aggcgacggg acggctgaaa ggcgccccgg ctcccgcacg
     8281 tctgaagctc accgacgtat tcgagcggct cgagttcacg cacgggcaca acgtgctgtt
     8341 gctgaacggc atgatgcggc tcgnnnnnnn nnnnnccgag agtccgcccc tatatcggca
     8401 tcggcggggg aattgccgtt ccccatgtcg aggtctggtt cccaggtgcg aatgccgtcg
     8461 aacgcacgaa cgagtaccaa tatgcggggc cggccgcaca ggcggtcgcc ggcttcgaaa
     8521 tcagtgccgg ccgggtgacc tacttcgtcg agtacaagtt cacctacgct tggatatcga
     8581 gcactctgac agccgaccaa tcctggaaga acttcaatat gccgggcgat cttttccgcc
     8641 aattccgcag gtggcttgcc ggtagctcgc caaagatcgg cacgatcagc acgacgctcg
     8701 gagcgcacca gatcgtggcc ggtgccggat acacgtggcg ggcgccgaaa gctgatccat
     8761 ctagcaggta aggcccgcac cgccgggcac gcccgagccc tttgcgacgt cgtcagaaag
     8821 ggctgaatgc tcgattttgc ccatcgcgcg aagccttttg tcgatcttcc aggccgaaac
     8881 agcaccccac acgcgttttc aatggggcac aaatggtcgc attgccgtcg tcaaccaact
     8941 attgaatcac ccatcgacaa ggctttcgat ccgaaataat tctttaattc caatatcgca
     9001 ggcttgcgcg ttatgatgga tgccgcggca atcggctcgg gagcgtcgca aggtactcgg
     9061 accaaatccg gcaacaccgg tcggcctgat tgcggtagta cccgttgcat ttttcggtta
     9121 ggacttccgc atgaactcga gaacgaagat gcgcggaaat ttggcccggg cgttctaaac
     9181 ccgttcgaac agcccaggtc ggcttcatcg caccgcctat cctttcgctg cggctccccg
     9241 gccgcagcgg gttgtgacaa caacggcttc accagaaaag gcagatcaat gaaggactcc
     9301 caactcgaaa agatgagcgt cgcagagctt cgcgacctcg tgacgcgcgc caatgcgatc
     9361 atctccaaca aggcccagca ggagaaggca caactccgcg agcgctggct gcgcgaggcg
     9421 aaggaggcag gcgtcccggt ggaaatccgc ttcactgata gccgcaaggg cgtccggaag
     9481 ggttccggcg cagacgtcaa gttccagcat cccgatgatc cctcgctcac ctggagcggt
     9541 cgcggtcgca agcccaagtg gctctctgag gccggcggca atatcgagcg tttccgcatc
     9601 gcctgacacc gcccgacgta atcgagcgat cgagaacgaa gcgccagccg gttcgccgtg
     9661 ctggcgcttc gtctttgtgc gcgacgtgtc cggcacgatg cacggcatcg tggcgctaag
     9721 gccacgccat caggcgtcat ctcacgcttg gcacgtgacg ccgcaccggc aagggtgagg
     9781 caacatgcta caagtatttc gctcgccggt gcctcacggc tgtccgattc caaggcgacg
     9841 ttcgagcggc aggcggcgca tttctctgag cggccggcga gagagggtca gcgaggttcg
     9901 agatggcacg acacgcgcgc tccgcgtcgt cgcacggcac gatcgacgtt gccggaattc
     9961 cgacgccggt cgagatccgc cggcatccgt cggcgcgacg gctgacgctg cgggtgagtc
    10021 ggacggaccg ctcggtagtc ctgactgttc cgaacggctc cgatctcggc gaagcgggcc
    10081 ggttcgcagg ccgccacgcc gattgggttc gaaagcgtct cgaatctgtg ccaagctccg
    10141 tcgcgtttgt ggacggcgcg gtcatccccg tccggggcat cgcgcactcg ttgaggttca
    10201 gcggagagac cagggccgaa ggcgtcgtcg tcgccaatcc aaccgaccag ggcgtgccag
    10261 ctgccctgct cgtatcgggc cgggtcgagc acgcgccaag gcgccttctc gaatggctga
    10321 tgcgcgaagc atgtcgcgac ctcgatgcat ccgtgcttca tcacgccggc cgcctcgggc
    10381 tcaggccgcg ccgcatcaca gtccgcgacc agcgcagccg atggggctcc tgctcgagca
    10441 caggccggct cgcattctcc tggcggctca ttctcgcacc gacgttcgtg ctcgactatg
    10501 ttgccgcgca cgaggtcgct cacctcgccg agatgaacca cgggcctcgg ttctggtcgc
    10561 tcgtcgagca gacgtgcccc gccacgcagc aggccaaggc ctggctcagc gcgcacggca
    10621 cgacactgca cggctacggc ccgccgagtt gaggatggct gcagtgggtg tcccgaccaa
    10681 tcgagcgact ggacaagcgc cgcgacagct gttatcggac gcgcattggg gccacacggc
    10741 cccttttcag tgacggcgct ccagaagtgg tggcgttgcg gcgggtgatt agctcagttg
    10801 gtagagcagc tgactcttaa tcagcgggtc gaaggttcga gtccttcatc acccaccact
    10861 tcgatcgggg cattgccccc gcgacgggcc ctcgatccgc attcgaagac ctgacaatga
    10921 accgattttt ctctggatca ccgctgctgc ggtcgcctgt cgtcattgca attccgatga
    10981 cgaacatgca ggtcgcgaac ggacgtcacg ctcgtcggat ccgacgtcga tcgaaccgtc
    11041 atgcaaatcc attgatttgg gcgatggtgg agtttgggcg atggcggaga cggagggatt
    11101 cgaaccctcg atacccgttt acacaagtat aacggtttag caaaccgccg ccttcagcca
    11161 ctcggccacg tctcctaagg ccccatgcgt ataggcgcgg ggccggcgaa ttgcaaccct
    11221 tgctgacacc cttgccagca catctacggc tcgactttgg aagattgtcg gcctgctcag
    11281 gccgcctagc ttgccttggt gtcgtagttg aggacgggcg ccagccagcg ctcggcttcg
    11341 gcgagcgtcc agcccttgcg gccggcgtag tcctcgacct gatcgcgctc gaccttgccg
    11401 acaccgaagt agtggctgtc ggcatgcgag aaatagagcc cggacaccga cgcacccggc
    11461 cacatggcgt agttctcggt gagttcgatg ccggcctgcg cttcggcatc gagcaggcgg
    11521 aacagggtcg ccttctccgt gtggtcgggc tgggccggat agccgggcgc cggccggatg
    11581 cccgtatact tctcggcgat cagctcctcg ttcgagagct tctcggtcgg ctgatagccc
    11641 cacagctcgc ggcggacccg ctcgtgcata cgctcggcga gcgcctcagc gagccgatcg
    11701 gccagcgcct tcagcatgat gcggccgtag tcgtcggtct tggcgatg
//
LOCUS       DAORNH010000129        11656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4174_length_11656_cov_2.771571, whole genome shotgun sequence.
ACCESSION   DAORNH010000129 DAORNH010000000
VERSION     DAORNH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4174_length_11656_cov_2.771571"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK264_13600"
     CDS             complement(<1..354)
                     /locus_tag="PK264_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD76943.1"
                     /translation="MVQTFDFIVIGAGVAGASAAAALCRRGKVCLIEQEAQAGYHSTS
                     RSAAVSSDAYGTRSWQILTSASRRFFLDPPAGFAERGLGWKLAQDGALVLNGTLPIST
                     FGGLKARGNPVGATGV"
     gene            complement(368..841)
                     /locus_tag="PK264_13605"
     CDS             complement(368..841)
                     /locus_tag="PK264_13605"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HRD76944.1"
                     /translation="MKPATAPAALRVQEALGDRYTVLEFDASTRTAADAAAAVGCSVA
                     QIAKSLIFRAAASGQAVLVIASGTNRVDERRVADLIGEPIARADADFVRGTTGYAIGG
                     VPPVAHRSPSIVLIDEALLAFDEIWAAAGTPNAVFKLKPGDLAGLTGGRVAIVAK"
     gene            complement(867..1226)
                     /gene="hisI"
                     /locus_tag="PK264_13610"
     CDS             complement(867..1226)
                     /gene="hisI"
                     /locus_tag="PK264_13610"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRD76945.1"
                     /translation="MEHGRAFAPKFDAAGVLAAVVTDAANGAVLMLAWMNAEALRLSI
                     ETGTAHFWSRSRNRLWRKGEESGNTLEIVEMRTDCDQDAIWLKVRVTGAGVACHTGAR
                     GCFYRAIDRAGVLHPAE"
     assembly_gap    1229..1238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1395..1856
                     /locus_tag="PK264_13615"
     CDS             1395..1856
                     /locus_tag="PK264_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRD76946.1"
                     /translation="MTALNDIYSTRILELAGNIPPAPRLAAPDATATAHSKLCGSTVT
                     VDLKVADGKVAAFGQTVKACLLGQSSASVMGRNIVGATPAELREVGRVMRDMLKRGGP
                     PPSGRWSDLAVLEPVREVRGRHASTLLVFDAVEAALDEIEAREGQGTATRA"
     gene            1856..2221
                     /gene="yidD"
                     /locus_tag="PK264_13620"
     CDS             1856..2221
                     /gene="yidD"
                     /locus_tag="PK264_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRD76947.1"
                     /translation="MPPAGSASPGRRLAAGIAKLPIHVYRYTLKPLIGYECRHLPSCS
                     AYGLDAIERNGAWRGLWLTVSRIWRCSPWGTSGHDEAPDIRAERHLLAPWRYGRWTRA
                     AIARQRNCNEQARETSVSD"
     gene            2281..2643
                     /locus_tag="PK264_13625"
     CDS             2281..2643
                     /locus_tag="PK264_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76948.1"
                     /translation="MRMLVLVLAILAGLKVAYQEYVYRSVTAEVIITAYRDRALEACR
                     THAWGKAVTADAWANPLSMTLSVGRSTLGSDAFQLDHAQWNARFKNPYLLITAGDGTA
                     EITCEFDIVNSVAAAQRR"
     gene            2787..3374
                     /locus_tag="PK264_13630"
     CDS             2787..3374
                     /locus_tag="PK264_13630"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRD76949.1"
                     /translation="MRRSAAIGMMALIAATGMMAGCANEAPRLDQSEVGRRMAGAEPR
                     LYRLGVGDKVKLTVFGEGDLSGTFEVNGAGQLPVPLVGDISARDKTIGELRDAIAARL
                     AKGYLKNPKVSIEILSYRPIYVHGEVRSGGEFAFKNGLRIRDAVAMAGGYSYRANQTF
                     VLVSRSGGAEEFRIDLPSDLAVMPGDNIRIPERFF"
     gene            3461..4777
                     /locus_tag="PK264_13635"
     CDS             3461..4777
                     /locus_tag="PK264_13635"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD76950.1"
                     /translation="MRVVLGLWITALVPGASAVRAFDVNPDPDRIDQTIAFRERLKDY
                     DSIKLYGAERVSDRTRTAYQPDGIRVGNLLIFPSIATAMTYDDNIHASAGPRIADMRF
                     DVTPSIRVRSSLPRHALDLSLGGRIVTYAENEELNHADFHVKAASAFHFDHAHTLSVS
                     MVSELLHLDRLDPTSPVSGRLPVSTWHSRASVGFTRDVGRLYGTVAVSADLWDNQDRI
                     LSSGAILDQDARDQRIFEGQVRLGYRISPGFELLGRFRGIRYLTPGDGARDWDGAGYE
                     LAVGLAMETSPLLRWRLLGGYGIRDFDQRGMAAFQTGLIEGEVQWLPTELMTVTASVS
                     RAMVQTLGDGAGDNGGRVDSAVIARIQYEVWRNLVLTVGGELRDYDYIGSDRNDRSAS
                     GRVGLEYLLNKQWKLSLGYEHQRRWSNLEEFNLTRNLFTFGAKLSF"
     gene            4786..7035
                     /locus_tag="PK264_13640"
     CDS             4786..7035
                     /locus_tag="PK264_13640"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRD76951.1"
                     /translation="MSTRPSESDTAADDGPGFGPKDAIAIIKRRGVLIAVVWTVLALI
                     AVGFAAALPSRYEAAATVQIDPRKRLIVNLEPVQSDLRPDTPTVESEVEVIRSRAIVT
                     RVIDKLGLRTDPEFAGPGPIRRVLIALGISKAPPPPLSVENLSAPIDPDRDEVAAGLE
                     KMLKVSRVRNTLLIEIRVTCGDPAKAARIANALAEAYLQEQLDAKAKATEQATGLIEA
                     RLDGLRQKLAHAERQVAMFKADNNIFDAEGQLLSEKKLARLMEQTVLVRSAAAEARAK
                     HEQVQQLLRKGADKGAVADVLASHTVRMLKDQLVKATRREAELATKYGSLHPEMLKIR
                     AEVADIRGQIADETRQIINNLATELKVAEDRERRLQDELARVMEQQNAAKEASVRLRE
                     LEREATSSRQVYEAFLARYKQTAETQGLELPDARIIEPAAVPLGPSSPKRKQLMAVGI
                     VGAFVLGLIAAFAVEFFTFGFGKPEQIEQSLGVEHLASLPFLPGARRDADRLRLARMV
                     LAEPTGAYTEAIRSLRHGLDARRRQAGSRIILVVSPQEADGKSMVAANLALHYALAGV
                     RTLLVDGDLRRKTLSATLGVDARAGLFDAIEFGLVPQSVALNDRSTGLALMPAGGQLA
                     RLAPPEALASERMVDVLGQLRQAFDVIVMDSPALLPVVDARIAAAHADQVLFVVRWGG
                     LDIADARRAIKALGVNARKIAGGVVNGIDLATADGVYDGEPAAAPITSRRTEPALGTA
                     AKTGGREAA"
     gene            7891..8850
                     /locus_tag="PK264_13645"
     CDS             7891..8850
                     /locus_tag="PK264_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRD76952.1"
                     /translation="MAAPGMTGMFESPRLPPAPASNWFATMLRTLGIVPPGPLLFIVS
                     GALMALGAMLLVRRRAAASRRDGSTDQPPAHSASPIIEAASTVRGVLLQLPAPQMAVA
                     ETVIAAAQELQPRAGERVAAAAASEPVASAPQPQAPATDTEALPTPDGDAGEACRELD
                     ATAAALSQIVGQMVADLGSDGELAAVLKEDLQGIDAELAAPKLRADIAAKRWSAARVP
                     LDAIIADLQRIRTLARIESERRIAAMPREPQLPVTRAEACEALGVNPQSDMKAIKKVV
                     DALRLTWHPDLAVDDAERRSREDRMKQINAAWDVLIQESRRLT"
     gene            8906..>9258
                     /locus_tag="PK264_13650"
     CDS             8906..>9258
                     /locus_tag="PK264_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76953.1"
                     /translation="MSYLIALRRYLRIPISLAHAIVARFGHLGYVVLATLLPIGLMIL
                     AYSSMFDDFSGAKPRPGDTWRILLLIVPTALAIVLAIALPFIDELGRIEDADRAKARR
                     GEVWAHWRLPSDAWA"
     assembly_gap    9259..9280
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9281..9808
                     /locus_tag="PK264_13655"
     CDS             <9281..9808
                     /locus_tag="PK264_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76954.1"
                     /translation="ELQREAGNTLLFGLGFAAIFGLVIGYASGPTFGLMVFAMIGLIA
                     LATSGRELLLGSSSAAGRMVDVYVSGDFVLANNQYSRLHHADRFGSGWNIASIALLPT
                     TPAILEITSHGFRSRTLGAMAGVRGGIEGVIAGAVNTSGGPPIIVRVPVPPDRKAEAE
                     TLIARFIKDRGAKAS"
     gene            complement(9812..10270)
                     /locus_tag="PK264_13660"
     CDS             complement(9812..10270)
                     /locus_tag="PK264_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRD76955.1"
                     /translation="MSQFLDIILAYGPWAWFIFAVILILLEMVVPGVHFLWYGIAAIL
                     VGLFSLATGIGWQTQLITFGILSVLTLFIVRRFGSPATAPTDEPTLNNRGEQFVGRTF
                     FLDEAIAAGRGKVRAGDTLWTVEGPDMPKGARVRVTAASGTVLTVERDRG"
     gene            complement(10458..>11656)
                     /locus_tag="PK264_13665"
     CDS             complement(10458..>11656)
                     /locus_tag="PK264_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRD76956.1"
                     /translation="PGTPAERRNGARVKDANVPPEGVYLPNNNRLAPHMRSPDYVQMS
                     TAAAITLGLMPGKMHGCGCTRCLNLLLTYPEGCRANCAYCGLARHREADRDYADRNFI
                     RVDWPAVPMAEIVEKVARDGEASPFHRMCISMITHPNSDADTVSVLKAWTSRIDPATI
                     PVSILSNPTTMTRADVARLKDLGADIFTVALDAVTPEIFDLTRGKGVQSPHTWAKYWE
                     ILEDARDIFGPERFGAHLIVGMGETESETLHLVQKLVDMGGHSHMFCFFPERGSLMDH
                     LPATPRDQWRRVQLARYLIDYCDVRVEHMRFDEEGRVADFGMGGADLDEIIQQGLAFR
                     TSGCPGKVREDLSACDRPYGDSPPSNIASYPFQPTGAHLRKIRHQLGRERPGESYVEG
                     EEIEAS"
BASE COUNT         1919 a         3911 c         3870 g         1924 t
ORIGIN      
        1 cacacccgtc gcaccaacgg gattgccacg cgctttcagc ccgccaaagg tgctgatggg
       61 cagtgtgcca ttcagcacta aagcgccatc ctgtgccagt ttccaaccca gtccccgttc
      121 ggcaaagccc gccggcgggt cgaggaagaa gcgacggctc gccgaggtga ggatctgcca
      181 ggatcgcgtg ccataggcat ccgagctgac cgccgccgag cggcttgtgc tgtggtagcc
      241 agcctgagct tcctgctcga tgaggcaaac cttgccgcgc cgacagagag cggctgccgc
      301 cgacgctccg gcgacgccgg cgccgatgac gatgaaatcg aacgtctgca ccatggctag
      361 cgccctgcta cttggcgacg attgcaacgc gcccgccggt gaggcctgca agatcgccag
      421 gcttcagctt gaagacggcg ttcggcgtgc cggccgcggc ccagatctcg tcgaacgcca
      481 gcagcgcctc gtcgatcagc acgatcgacg gactcctatg ggcaaccggg ggtacgccgc
      541 cgatggcata gcccgtggtg ccgcgcacga agtcggcgtc ggctcgcgca atcggttcgc
      601 cgatcaggtc ggcgacgcga cgctcgtcca cccggttggt gcccgaggcg atcaccagaa
      661 cggcctgccc cgaggccgcg gcgcggaaga tcagcgactt ggcgatctgg gcgactgagc
      721 agccgacggc ggcggccgca tcggcggccg tgcgcgtgct cgcatcgaac tcgagcaccg
      781 tgtagcggtc gccaagcgcc tcttggacac gcagggccgc aggggccgtc gctggtttca
      841 tgttcgctgg tcctgggtct ggttactcat tcggcgggat gcagaacgcc cgcccgatcg
      901 atggcacgat aaaagcagcc gcgcgcgccg gtatggcagg cgacgccagc gccggtgacg
      961 cgcaccttga gccagatcgc gtcctggtcg cagtcggtgc gcatctcgac gatctcgagc
     1021 gtattgccgc tctcctcgcc cttcctccag aggcgattgc gcgagcggct ccagaaatgg
     1081 gctgttccgg tctcgatcga gaggcgcaac gcctcggcgt tcatccaggc aagcatgagg
     1141 acggcgccgt tcgcggcatc ggtcacgacg gccgccagca cacccgcggc atcgaatttc
     1201 ggcgcgaagg cccggccatg ctcgatctnn nnnnnnnngt ggcgccgcgg ggcgcaaagg
     1261 tcactttgtt cggctcggac atggtctgat ctcgaggagc tgcccctcgg ctaccgccac
     1321 ctatgacagc agctatgcag ccggctcggc ccgcgcatct tgcattccgg ggcgcgaggc
     1381 gcaatatcgc tgcgatgacc gccctcaacg acatctacag cacccgcatc ctcgagcttg
     1441 ccgggaacat tccgccggcg ccgcgacttg cagcacccga tgcgacggcg acggcacact
     1501 cgaagctgtg cggctcgacc gtgacggtcg acctcaaggt tgcagatggc aaggttgcgg
     1561 ccttcggcca gacggtgaag gcctgccttc tcgggcaatc gtcggcctcg gtgatgggac
     1621 gcaacatcgt cggagcgacg ccggccgagc tgcgcgaggt cggccgcgtg atgcgcgaca
     1681 tgctgaagcg gggcggcccg ccgccatccg gtcgctggtc ggacctcgcc gtgctcgagc
     1741 ccgtgcgcga agtgcggggc cggcatgcct cgacactcct cgtcttcgat gcggtcgagg
     1801 ccgctctcga cgagatcgaa gcgcgcgagg ggcagggcac tgccacacga gcctgatgcc
     1861 accggccgga tcagcaagtc cggggcggcg cctcgctgcc gggatcgcga agctgccgat
     1921 ccatgtctac cgctacaccc tgaagccgct catcggctat gagtgccgcc acctgccgag
     1981 ctgctcggcc tacggcctcg atgcgatcga gcgcaacggc gcctggcgcg gcctctggct
     2041 caccgtctcg cggatctggc ggtgtagtcc gtggggaacg tcgggacacg acgaggctcc
     2101 ggatatccgg gccgagcggc acctgctcgc cccttggcgg tacggccgct ggacgagggc
     2161 ggccattgca cgacagcgca actgcaatga gcaggcgcgc gagacaagcg ttagcgattg
     2221 attaaccagg gcccggcaag ctgcacgttc ccttccactg agaggtgtgc ggcgggtgcc
     2281 atgcgcatgc tcgtcctcgt tctggcgatc ctggccggcc tcaaggtcgc ctaccaggag
     2341 tacgtctatc gaagcgtgac ggcggaggtg atcatcacgg cctaccgcga ccgtgctctc
     2401 gaagcctgcc ggacacacgc gtgggggaag gcggtgacgg cggacgcctg ggccaacccc
     2461 ttgtcgatga cgctctccgt cggccgctcg acgctgggct cggacgcgtt ccagctcgat
     2521 cacgcgcagt ggaacgcccg cttcaagaat ccctacctgc tcattaccgc gggcgacggc
     2581 acggccgaga tcacctgcga gttcgacatc gtcaacagcg ttgcggctgc acaacggcgc
     2641 taattggtgg ccaaacacct cgaagtgaac aattttcggc ttgtttggcc gagttcctaa
     2701 acctggattt tacctaagtt ggatcatctg gacggtgtgg gcagaggcgt ttctgccccg
     2761 ctcggttgaa aacgagaccg tccgtcatgc gtaggtcggc agcaatagga atgatggcgc
     2821 tcatcgcggc gaccggcatg atggccggtt gcgcgaacga ggcgccgcgc ctggatcaat
     2881 ccgaggtcgg ccgccgcatg gcgggtgccg agccgcgcct ctacaggctt ggcgtcggtg
     2941 acaaggtgaa actcaccgtc ttcggtgaag gcgacctctc cggcaccttc gaggtgaacg
     3001 gcgcggggca gctgcccgtg ccgctcgtcg gggacatctc cgcccgcgac aagacgatcg
     3061 gcgagctccg tgatgcgatc gcggcccgcc tcgccaaggg ctacctcaag aacccgaagg
     3121 tctcgatcga gatcctttcc taccggccga tctacgtgca cggcgaggtg aggagtggcg
     3181 gcgagttcgc cttcaagaac ggcttgcgca tccgtgacgc cgtcgccatg gccggcggtt
     3241 actcctaccg cgccaaccag accttcgtac tcgtctcgcg gagcggcggc gcagaggagt
     3301 tccgaataga cctgccgagc gacctcgcag tcatgcccgg cgacaacatc cgcatcccgg
     3361 agcgcttctt ctgatgctcc tcgcggtcca cagacctgcc gaccggttgc gccggcgcct
     3421 cgatgccttc cggcatcgcc tggcgcagga gtggcggacc atgcgcgtgg tgctgggtct
     3481 ctggatcacg gccctcgttc cgggcgcgag tgccgtacgc gccttcgatg tcaatcccga
     3541 tccggaccgc atcgaccaga cgatcgcctt ccgcgagcgg ctcaaggact acgacagcat
     3601 caagctctac ggcgccgagc gcgtcagcga ccgcacgcgc acagcctacc agcctgacgg
     3661 catccgcgtc ggcaacctgc tgatcttccc gtcgatcgcg acggcgatga cctacgacga
     3721 caacatccac gcgtccgccg gcccgcgcat cgcagacatg cgcttcgacg tcacgccctc
     3781 gatccgcgtg cgctcgtcgc tgccgcgcca tgcgctcgat ctctcgcttg gcggccgcat
     3841 cgtcacctat gccgagaacg aggagctgaa ccacgccgac ttccacgtga aggcggcgag
     3901 cgcgttccat ttcgaccatg cgcacacctt gtcggtgagc atggtgtcgg agctccttca
     3961 tctcgaccgt ctcgatccga cctcgccggt gagcggccgg ctcccggtca gcacgtggca
     4021 ctcgcgcgcg agcgtcggct tcacgcgcga tgtcggccgc ctctacggca ccgtggccgt
     4081 ttcggccgat ctctgggaca accaggatcg catcctttcc tccggcgcca tcctcgacca
     4141 ggacgcccgc gaccagcgca tcttcgaagg ccaggtgcgc cttggctatc gtatatcgcc
     4201 cggtttcgag ctgctcggcc gcttccgcgg catccgctac ttgacgcctg gtgacggcgc
     4261 ccgcgactgg gacggcgccg gctacgaact cgccgtcggc ctcgccatgg agacgagccc
     4321 gctgctgcgc tggcgtctcc tcggcggcta cggcatccgc gacttcgacc agcgcggcat
     4381 ggcggcattc cagaccggcc tcatcgaggg cgaggtgcag tggctgccga ccgagttgat
     4441 gaccgtcacg gcgagcgtca gccgcgcgat ggtgcagaca ctcggtgacg gcgccggcga
     4501 caacggcggc cgcgtcgata gcgccgtcat cgcccgcatc caatacgaag tgtggcgcaa
     4561 tctcgtgctg acggtcggcg gcgagctgcg cgactacgac tacatcggct cggatcgcaa
     4621 cgaccggagc gcgagcggcc gcgtcgggct cgagtacctg ctcaacaagc agtggaagct
     4681 gtcgctcggc tacgagcatc agcgccgctg gtccaacctg gaggagttca acctcacacg
     4741 gaacctgttc acgttcggcg cgaagctgag cttctgaggc aggcgatgtc cacgagaccc
     4801 tcagagagcg atacggcggc cgacgacggc ccgggcttcg gcccgaagga tgcgatcgcc
     4861 atcatcaagc ggcgcggcgt gctcattgcc gtcgtatgga ccgtgctggc cctgatcgcg
     4921 gtcgggttcg ccgcagccct gccgtcgcgc tacgaggcgg cggcgaccgt gcagatcgat
     4981 ccgcggaagc gcctcatcgt caatctcgag ccggtacagt ccgatctgcg ccccgatacg
     5041 ccgaccgtcg aaagcgaggt cgaggtgatc cgctcgaggg cgatcgtcac gcgcgtgatc
     5101 gacaagctgg gtctgcgcac cgatcccgag tttgccggac ccggacccat ccgccgcgtc
     5161 ttgatcgcgc tcggcatctc gaaggcgccg ccaccgccgc tctcggtgga aaacctttcg
     5221 gcgccgatcg atcctgaccg ggacgaggtc gcagccgggc tcgagaagat gctgaaggtc
     5281 agccgtgtgc gcaacacgct gctgatcgag attcgtgtaa cctgcggcga tcctgcgaaa
     5341 gccgcacgca tcgccaatgc ccttgccgag gcctacctcc aggagcagct cgacgccaag
     5401 gccaaagcca cggagcaggc gaccggcctc atcgaggcgc gcctcgacgg cctcaggcag
     5461 aagctcgcgc atgccgagcg gcaagtcgcc atgttcaagg ccgacaacaa catcttcgat
     5521 gccgagggcc agctcctctc ggaaaagaag ctcgcccgcc tcatggagca gacggtgctc
     5581 gtccgctcag cagccgccga ggcgcgcgcg aagcacgagc aggtgcagca actcctcagg
     5641 aagggcgcag acaagggagc ggtcgccgac gtgctcgcga gccacaccgt gcgcatgctg
     5701 aaggaccagc tcgtcaaggc gacccgccgc gaggcggagc ttgcgacgaa gtacggatca
     5761 ctccatcccg agatgctgaa aatccgcgcg gaggtcgccg acatccgcgg ccagatcgcc
     5821 gacgagacgc gccagatcat caacaacctc gcaaccgagc tcaaggtcgc cgaggatcgc
     5881 gagcgacgcc tgcaggacga gctcgcccga gtcatggaac agcagaacgc ggcgaaggag
     5941 gcgagcgtcc gcctgcgcga gctcgaacgc gaggcgacat cgagccgtca ggtctatgag
     6001 gcgttcctcg cccgctacaa gcagactgcc gaaacgcagg gcctcgagct cccagatgcc
     6061 cgcatcatcg agccggccgc ggtgccgctc ggaccgagct cgccgaagcg caagcagctg
     6121 atggccgtcg gcatcgtcgg cgccttcgtg ctcggcctca tcgcggcctt tgccgtcgag
     6181 ttcttcacgt tcggcttcgg caagcctgag cagatcgagc agtcgctcgg cgtcgagcat
     6241 ctggcctcgc tcccgttcct gccgggcgcc cggcgggacg cagaccggct ccggctcgcg
     6301 cgcatggtgc ttgccgagcc gacaggcgcc tataccgagg cgatccgctc gctccggcac
     6361 ggcctcgatg cgcgccgccg ccaggcgggt tcacgcatca tcctggtggt ctcgccgcag
     6421 gaggcggacg gcaagtcgat ggtagcggcg aaccttgcgc tccactatgc gctcgccggc
     6481 gtgcgcacgc tgctggtcga cggcgatctc cgccgcaaga cgctttcggc gacgctcggc
     6541 gtcgacgcgc gcgcggggct gttcgatgcg atcgagttcg gcttggttcc gcagtcggtc
     6601 gcgctcaacg atcgatcgac ggggttggcg ctcatgccgg cgggcggcca gcttgcgcgg
     6661 ctcgccccgc ccgaggctct ggccagcgag cggatggtcg acgtgctcgg tcagctgcgg
     6721 caggccttcg acgtgatcgt catggattct ccggccctcc tgccggtagt cgatgcccgc
     6781 attgctgcag cgcacgccga tcaggttctc tttgtcgtgc gctggggcgg gctcgacatc
     6841 gcggatgccc gccgagccat taaggcgctc ggcgtgaacg cgaggaagat tgcgggcggt
     6901 gtcgtcaacg gcatcgatct cgcgacggcc gacggtgtct acgacggcga gcccgccgcc
     6961 gcaccgatca caagccgccg aactgagccg gctctcggga cggcagccaa gactgggggg
     7021 cgcgaggcgg cgtaactggg agcgacacct tatcgccgag gaaaaggact ttctgccgca
     7081 ttgtggccaa tctccctgcc gatggtcgga aaacatcgac gatcttcggg ggacggcgtg
     7141 gcgaggggta ccggggcgaa gtccagcctc cgtgactgta gtcttatgct ggccgcggca
     7201 ttcgggctcg gtgccgggct cggcgccgac gtgcgcgccc aagagagggc ctcggagcag
     7261 gcctcgctgc cgttccattg cgaatacgac ggccatcgcc ctgtcgtgac gcccgccgga
     7321 cctcaggccc acgacatcac aagtgaacgc gaaaatctcc ctttcacggc ctgtcgcggc
     7381 ccgggcagcg aggggtgccg cacctggatg gtgcatcgct tcgagctgcg gtgccgcacg
     7441 gcggtcgtgc cgtgggttgc cattgtcgcc cgcgcgccgc accttccggg tcgagtcacg
     7501 atctctgacg gcgcgctcac tctcaggctt gatgcgtcag gcaatgcgcc gtacctcggg
     7561 ccgtgccccc ggccgggcga taagccgctc ggtcatgcga caagcgctcc atggcctcga
     7621 ttgcccctcg ataggcgctg tttcgagccg cgctcgtcct ggaccgacgg ctggtccgtc
     7681 acctttccga aggggtatgc gcctgttgca gggatcggcg cgcgcttcat caccacgtcg
     7741 acgcacgggg ccggatccct cgattcgccg acggacgctg ccggcaagca tcgcagcggc
     7801 ggcatgccgg taacctgggt gcgacggaca ccgaccggat cagagcctca accggctcgc
     7861 gcggcaggca cgagcgatca cgatccgttg atggcagcgc caggcatgac ggggatgttc
     7921 gaatctccac gcttgccgcc cgcaccggcc tcgaactggt tcgctaccat gctgcgcacg
     7981 ctcggaatcg tgccaccagg gccgctcttg ttcatcgtct cgggtgcctt gatggctctc
     8041 ggtgcaatgc tgctcgtccg gcgccgggcg gccgcctcgc gtcgcgacgg atcgacagac
     8101 cagccacctg cacattcggc atcgccgatt atcgaggctg cgtcgaccgt acgcggcgtg
     8161 ctcttacagt tgccggcgcc gcagatggcg gtcgccgaga ccgtaattgc ggctgctcaa
     8221 gagctccagc cgcgcgccgg cgagcgtgtt gcagctgccg ccgcatcgga gccggttgcc
     8281 tcggcacctc agccgcaggc tccagcgacg gatacggagg cgctgccgac gcctgatggc
     8341 gatgcaggcg aggcttgccg tgagctcgac gcaacggctg ccgcattgag ccagatcgtc
     8401 ggccaaatgg tcgccgatct cggttccgac ggggagctcg cggccgtgct caaggaggac
     8461 ctgcagggta tcgacgccga gcttgcggca ccaaagctgc gcgcggacat cgcggccaaa
     8521 cgctggtccg cggcgcgcgt cccactcgac gccatcatcg ccgatctgca gcgcattcgc
     8581 acgctcgcac ggatcgagag tgagcgtcgt atcgcggcga tgccgcgtga gccgcagctt
     8641 ccggtaactc gcgccgaggc gtgcgaggct ctcggcgtca atccgcagtc cgacatgaag
     8701 gcgatcaaga aggtcgtcga tgcgctgcgc ttgacgtggc accctgatct cgctgtggac
     8761 gatgctgagc gacgttcgcg cgaggatcgc atgaagcaga tcaatgccgc ctgggatgtc
     8821 ctcatccagg agagccgccg tttgacctga cggcatcagc tgcattctga tctgaacttg
     8881 gacccgatga tggtggaggc tgcgtgtgag ctacctgatc gcactccgtc gatatctgcg
     8941 gatcccgatc tcactggcac acgcgatcgt cgcccggttc ggccacctcg gctacgttgt
     9001 gctggcgacg ctcctgccta tcggcctgat gatcctcgcc tacagttcga tgttcgacga
     9061 tttctccgga gccaagcctc gccccggtga cacgtggcga atcctgctgc tcatcgtgcc
     9121 gactgcgctc gccatcgtac tggcgatcgc gcttccgttc atcgacgagc tcggtcgcat
     9181 cgaggacgcc gatcgggcga aggcgcgacg cggcgaggtc tgggcacact ggcgcctgcc
     9241 gagcgacgct tgggcacann nnnnnnnnnn nnnnnnnnnn gagctccaga gggaggcagg
     9301 caacacgctg cttttcgggc tcggcttcgc cgccattttc ggtcttgtca ttggctatgc
     9361 ctcgggcccg acgttcggcc tcatggtgtt cgcgatgatc gggctgatcg cgctcgcgac
     9421 ctcgggtcgc gagctactgc tcggctcgtc cagcgccgcc gggcgcatgg ttgatgtcta
     9481 tgtctcgggc gattttgtcc ttgctaacaa ccagtactcg cgcctccacc atgccgacag
     9541 gttcggcagc ggatggaaca tcgcttccat cgcgctgctg ccgacgacac ccgcaattct
     9601 cgagatcacg agccacggct tccgctcgcg gacactcggc gcaatggccg gcgtgcgggg
     9661 cggcatcgag ggcgtgatcg caggggcggt gaacacgtcg ggcggtcccc cgatcattgt
     9721 ccgcgtgccg gtgcccccag atagaaaggc cgaggccgaa accttgatag cgcgcttcat
     9781 caaggaccgc ggcgcgaaag cgtcgtgacg ctcagccgcg atcgcgctcg accgtcaata
     9841 cagtgccact cgccgcagtg accctgacgc gggcgccctt cggcatgtcg gggccttcga
     9901 cggtccagag cgtgtcgccg gcccgcacct tgccgcggcc ggcggcaatc gcctcatcga
     9961 ggaagaaggt ccgcccgacg aactgctcgc cgcgattgtt gagggtcggc tcgtcggtcg
    10021 gcgcagttgc aggcgagccg aagcggcgca cgatgaagag ggtgagcaca gagaggatgc
    10081 caaaggtgat gagctgcgtc tgccagccga tgcccgtcgc cagcgagaac agcccgacga
    10141 ggatcgcggc gatgccgtac caaagaaagt gcacccccgg gacgaccatc tcgagcagga
    10201 tcaggatcac ggcgaaaatg aaccatgccc acgggccgta ggccaggatg atgtccagaa
    10261 attgcgacat ggggtccctc atcttctgct cgaatgcaac taagtcacta gcagaaatcg
    10321 attgtgaagc ggtaagggtc ccatggccga ttggacggga gcatgatggc gaattggaag
    10381 agggccggcg gcctgtccag gaattcgctc atacaaccta tcagtgcgta tcggaccgag
    10441 gaagtgcgcc gcgagcgtca cgacgcctcg atttcctcgc cctcgacgta gctctcgccg
    10501 ggccgctcgc ggccgagctg gtggcggatt ttgcgcaaat gcgcgcccgt cggctggaag
    10561 gggtagctcg cgatgttcga aggcgggctg tcgccgtagg gccggtcgca ggcggagagg
    10621 tcctcgcgga ctttgcccgg gcagcccgag gtgcggaagg cgaggccctg ctggatgatc
    10681 tcgtcgagat cggccccccc cataccgaag tcggcgacgc ggccctcctc gtcgaagcgc
    10741 atgtgctcga cgcggacgtc gcagtagtcg atgaggtaac gggcaagctg cacacgccgc
    10801 cactgatcgc gtggcgtcgc ggggaggtgg tccatcagcg agccgcgctc tggaaagaag
    10861 cagaacatgt ggctatggcc gcccatgtcg acgagcttct ggacgagatg caacgtctcg
    10921 gactctgtct cgcccatgcc gacaatgagg tgcgcgccga acctctcggg cccgaagatg
    10981 tcgcgcgcat cctcgaggat ctcccagtac ttggcccagg tgtgcggcga ctgcacaccc
    11041 ttgccgcgcg tcagatcgaa gatttccggc gtcacggcat cgagcgctac ggtgaagatg
    11101 tcggcgccga ggtccttgag gcgcgcgacg tcggcgcgcg tcatggtcgt ggggttcgag
    11161 aggatcgaga ccgggatcgt cgccggatcg atgcggctcg tccaggcctt cagcaccgag
    11221 accgtatcgg catccgagtt cggatgcgtg atcatcgaga tgcacatgcg gtggaagggg
    11281 ctcgcctcgc cgtccctggc gaccttctcg acgatctcgg ccatcggcac ggccggccag
    11341 tcgacgcgga tgaagtttcg atcggcgtag tcgcggtcgg cctcgcgatg gcgtgcaagc
    11401 ccgcagtagg cgcagttggc gcgacagcct tcggggtagg tgagcagcag gttgaggcag
    11461 cgcgtgcagc cgcagccgtg catctttccc ggcatgaggc cgagcgtgat ggccgccgcc
    11521 gtcgacatct gcacatagtc cggcgagcgc atgtgcgggg cgagccggtt gttgttcggc
    11581 aggtacacgc cctcgggcgg cacgttggcg tccttcacgc gcgcgccgtt gcgccgctcg
    11641 gccggtgtgc ccggcg
//
LOCUS       DAORNH010000130        11625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4188_length_11625_cov_2.780380, whole genome shotgun sequence.
ACCESSION   DAORNH010000130 DAORNH010000000
VERSION     DAORNH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4188_length_11625_cov_2.780380"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PK264_13670"
     CDS             complement(<1..531)
                     /locus_tag="PK264_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRD76957.1"
                     /translation="MSGPAYQRPETIEEALGLLARDGARVLAGGTDLIVQMKEGRRTP
                     KAIVDVKRVGQLTAISERPDGSLAIGAAASATSIARHQRVRNDYPALAASVGLIGSLQ
                     IQSRASLGGNIANAAPSGDAIPAAMVLGAEVEILGGAGVRSVPLASIFTGPGQTTIAP
                     GEMLVAIVLPPRRPRSA"
     gene            complement(537..1547)
                     /locus_tag="PK264_13675"
     CDS             complement(537..1547)
                     /locus_tag="PK264_13675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD76958.1"
                     /translation="MPRHASIWRSLRPAALVAAIAVMVSLAAALRVPAQEKWPTKPIT
                     IVMGFPAGSGVDVIARLLQSSMEKQLGTTLVFDYKVGAGGNNASALVAKAAPDGYTIL
                     LGTSATHGVNAALYKRLPFDVEADFTPIAPLNDVSNVLTINPKVIDAKNIPEFIAKVK
                     AMPGKLNFASTGNGTGTHLAFATFVKAAGLDMVHIPYKGGPEALQSVVTGETCCIMNQ
                     VQTVLGQVKAGKVRMLGVTTAKRVAAVPDVPTIDESGVKGYESFIWFGLFGPKGLDPA
                     IAEKINAAVRTSLANPDIQKRMAELGNTPRSESVAEFRVTVKADRAKWAEVVKASGAS
                     ID"
     gene            1718..2719
                     /locus_tag="PK264_13680"
     CDS             1718..2719
                     /locus_tag="PK264_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD76959.1"
                     /translation="MMSRSRRALMLGALAIAAACAAPTAGVAQSKWDLSTVWPDGNFH
                     TKNAVRFAADVKAATKGAVDIAVKSGGQLGFKGPEHLRAVRDGLVPIADILNIQQIGD
                     EPLLGIESIAFLVSSPAELKLLHKHIRPIYEQIAARNNQKILYMVPWPTQYLHLKVKS
                     NTLDGLKGVKIRVPDKGAADMLTLVGMAPVLIPWGETIPALASGVVAGVSTSAVSGVD
                     GKFWEFLKYVYPTNHVWSSQMMNVNLDAWKKLSPEHQKAIEDLARKLEPEFWDVSVKA
                     DADSLAKLKAQGMEVVELPPAMMADLRAKTANILNEYMKRVPASQPAIKAYLAEVKR"
     gene            2756..3895
                     /locus_tag="PK264_13685"
     CDS             2756..3895
                     /locus_tag="PK264_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HRD76960.1"
                     /translation="MSMPPPARVGDPVAEIDTPALVIDLDAMERNIARLAAFAAKTGV
                     KLRPHAKTHKSPDIGRRQIAAGAVGLCCQKVSEAEAMVAGGIGDVLVSNEVFGARKLD
                     RLASLSRRATVAVCADNVEAIEAIAAAAGRQGVRITTLVEIDVGANRCGVAPGEPAVV
                     LARLIASKPNLKFGGLQAYQGRAQHLRTIAERRAAIDHAIEVTRRTVELLEATGLPPA
                     IVGGAGTGTYDLEGSSGVYTELQAGSYVFMDADYARNKRADGRPYDTFEHALFVIATV
                     MSLPVAERAVIDAGLKTFATDAGMPEPRGLDGASYIRPSDEHGIIDLTKSNHRLALGD
                     RVALIPGHCDPTVNLHDWYVGVRGYGTPNAHVECLWPVAARGAIF"
     gene            4455..4904
                     /locus_tag="PK264_13690"
     CDS             4455..4904
                     /locus_tag="PK264_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76961.1"
                     /translation="MHRLVTGPNIGTPYIGEALFSAPASFNLGKIADQQNWMAAAAQY
                     FTPLLEKQRVRDNYVSPPPPPRPDNSALVMVGLGAAGALGILFIMLKTSRGGVGSPSI
                     LERSALASMIAALTSSILVAFGVADGVKLVTATLAAFSVGIVVSMAT"
     gene            5058..5690
                     /locus_tag="PK264_13695"
     CDS             5058..5690
                     /locus_tag="PK264_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76962.1"
                     /translation="MPKRLVSDRRISRIELDWKGGWARQSCPPEAVSVFVVTRPELCR
                     AVATNSSDQQAVVHGTGLIEVRAWPRSGNPETLFRGNISFNLGQMVGAQGQSVSGQAR
                     AFVTQITAKLEDFLLRHAAASHKIDLQNQIVLTAGLIAAAAFGLFLYLTSKGSAEGDG
                     GRILRKLLFSLAIGATSAAIAAHLGHGEDLKVAAVSVGAFMVTLVVSAAF"
     gene            complement(6311..8572)
                     /locus_tag="PK264_13700"
     CDS             complement(6311..8572)
                     /locus_tag="PK264_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76963.1"
                     /translation="MSNKTPYEQLRDGELPGMVSWYDPRVLARIGVRTIVSSVFGQYA
                     DQRLLQAATDQSTVTRLIERYDYSDPSSSDPERRLTTDENGAIWIDYVADTGDGFEST
                     YTMAYLLAKPTLDMGEVGTLKAGEILVMGGDQCYPQATREEYKKRLQVPFGWAYNVSE
                     PQRKLFAIPGNHDWYDGLTAFDSLFCSARDRLSSGRGNVIGGWQCQQHRSYWALKLPH
                     KWWIWGTDIQFSSYLDNAQVNYFQKIAEEMGPDDKLILCIAEPSWMIADLEGGDEEQN
                     FFKITSIARQRGVRICAVVAGDWHHYNRYYQPDLDVHFFTAGGGSFLHPTHTLKNEIK
                     ITWPERVQWVEQSPQPTVAGPSGDWSPAEYSARLKRPDGPVGSVREAVREALRPGSPR
                     TATAPRQRDTKCYPDKSTSWWLSLRNLFFPLYNIPFGIGIGLIYWLITWQFHTVVRAH
                     DISSGEIDHISFATIPAQLAWMPVYILQATLSIGFVAMVFGLAFVLLSYVQVHERRGI
                     RAGLFRTFVGGGHFLAHMIAAFALFLTFTAFNNWLAPHAARGASAMLSADWMPTIVRP
                     VVQKTLEPISTYRTETRTRIAAEEGRSRSAPSSVSSVPPPPGAALPPLQPITATQTSV
                     DPKAARELIGLLYPFQMVLLGGLLGGFIWGLYWVITGVLWRMHTEDAFGALRIADYKN
                     FLRLKLEPDQVTIYPLGVDRIVRASGWAEPAKDKAPLPNNPALVPADGKQPAVRLIEA
                     PIVIRASQQGPTS"
     gene            complement(8690..9649)
                     /locus_tag="PK264_13705"
     CDS             complement(8690..9649)
                     /locus_tag="PK264_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD76964.1"
                     /translation="MTAGVANAEVGELNVAKQFGIAYLPLMIMEADGLVEKHAKAAGV
                     ELKVTWRTLAGGAPMNDAILSGSLHFASGGVGPLTTLWARTKGSIDVKAVAAMNSMPI
                     YLNVRNPAAKSLKDLTEKDRIALPAVKVSIQAITLMMAAEKEFGVGNHAKFDALTLAM
                     SHPDAQAALLSGASEVTGHFSSPPFQYQQLKTPGIRTILNSYDVLGGPGTFNLLWTTK
                     KFHDENPKVYAAFVAALGEAIETINKGKPKAALRYLEISRDKKSTVEEILAMLDDPAI
                     EFTMTPKGVMRYAEFMHKIGLIKVKPGDWKDLFFPNAHGLKGS"
     gene            9870..>11625
                     /locus_tag="PK264_13710"
     CDS             9870..>11625
                     /locus_tag="PK264_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76965.1"
                     /translation="MFRWFETRIDPFPEEPIRQPPATLLAFFWHYLRPVAWAFAALTA
                     MTLIMAVIEVAIMAFIGRVVDLMKEAKTPHTFFADHGLELALMAGIALIVRPLVSALA
                     DFTKQQMIQGPVNIRTRWLAHRYVLRQSISYFQNDFAGRVATRVIQVGPAVRDVAGNT
                     CDVVVWVVVHWIGAFALFFAADWRLVIPLTLWLIGYIIALTYFVPRIERASHDMSEAR
                     SMLTGRIVDSYTNIATVKLFAHTDHEDRYAREAQRQFLERYMAQLRLNTLMEIVLWSL
                     NGLVLVATLGLAVWLWTGNLITIGAITVVSTLVIRLMNMSGWFMWTLAGIFDNIGVVQ
                     ESMQTIARPLQVVDSPGAKPIDVPKGAIRYEAIGFHYEKAAVENVESAGRKSFWDSRK
                     AARAKADPGEERAEPAKLAGMPSLPRRSVIERLTLDIAPGEKVGLVGRSGAGKSTLVN
                     LLLRFYDLEKGRILIDGQNIAEVTQDSLRANIGVVTQDTSLLHRSVRDNIRYGRTEAT
                     EAEIVEAAKRAEAHEFILGLEDGRGRRGYDAHVGERGVKLSGGQRQRIAIARVLLKNA
                     PILVLDEATSALDSEAEAA"
BASE COUNT         2006 a         3763 c         3762 g         2094 t
ORIGIN      
        1 cgccgagcgc ggccgccgcg gcggaaggac gatggcgacg agcatctcgc cgggtgcgat
       61 cgtcgtctgg ccggggcctg taaagatgct cgcgagaggt acgctccgga cgcccgcgcc
      121 acccagaatc tcgacctcgg cgccgagcac catggcggca gggatggcgt caccggaggg
      181 cgctgcattg gcgatgttgc cgccgaggct cgcgcgtgac tggatctgca ggctgccgat
      241 caggccgacg gaggctgcaa gtgccggata gtcgttacgg acgcgctgat gccgcgcgat
      301 gctggtcgcc gaggccgcag cgccgatcgc gaggctgcca tccggccgct cggaaatggc
      361 cgtgagttgc ccgacgcgct tcacgtcgac gatcgccttg ggggtgcgcc ggccctcctt
      421 catctgcacg ataagatcgg tgccgccggc gagcacgcgc gcgccgtcgc gggcgaggag
      481 gccgagcgcc tcctcgatcg tctcgggtcg ctgatatgcg ggtccgctca cgggcatcaa
      541 tcgatcgagg cgccggaggc cttcacgacc tcggcccatt tggcgcgatc ggccttcacg
      601 gttacccgga actcggcgac gctctcagag cgcggcgtgt tgccgagctc cgccatgcgc
      661 ttctggatgt ccggattggc gagcgaggtg cgcaccgccg catttatctt ctcggcaatc
      721 gcgggatcga gccccttcgg accgaagagg ccgaaccaga tgaagctctc gtagcccttg
      781 acgccggact cgtcgatcgt cggcacgtcg ggcacggccg cgacacgctt cgccgtcgtc
      841 acgccgagca tgcgcacctt gccggccttc acctgtccga gcacggtctg cacctggttc
      901 atgatgcagc acgtctcgcc cgtgacgacg gactgcagcg cctcgggccc gcccttgtaa
      961 ggaatgtgca ccatgtcgag gccggccgcc ttcacgaagg tcgcgaaagc gaggtgcgtg
     1021 cccgtgccgt taccggtcga ggcgaagttg agttttcccg gcatggcctt caccttggcg
     1081 atgaactcgg gaatgttctt ggcatcgatg acctttgggt tgatcgtcag cacgttcgag
     1141 acgtcgttga gcggcgcgat cggcgtgaag tcggcctcga cgtcgaaggg caggcgcttg
     1201 tagagcgcgg cgttgacgcc gtgcgtggcc gaggtgccga ggaggatcgt gtagccgtcg
     1261 ggcgcggcct tcgcgacgag tgccgaggcg ttgttgccgc ctgcgccgac cttgtagtcg
     1321 aacaccagag tggtgccgag ctgcttctcc atcgagctct gcagaagccg ggcaatgaca
     1381 tcgacgccgg agccggccgg gaagcccatg acgatcgtga tcggcttcgt cggccacttc
     1441 tcctgtgcag gcaccctcag cgcagcagca agagagacca tgacagctat agcggccacg
     1501 agcgcagcag gacgaagact gcgccagatc gatgcgtgac gaggcatgat agagtagtcc
     1561 ctcgaagtgg gtttcgtccg gccacaatgc cccgtcctca gcagccttgc aacgcgctcg
     1621 atcggggaac atgccccggc cttgagccat cgggctgtcc gtggcaccat ctttgcggcg
     1681 cgcgggccgg atgcccgccg cgacagggag ctcttacatg atgtctcgat cacgacgcgc
     1741 gctgatgctc ggcgcgctgg ccatcgcagc cgcatgcgcc gcacctactg caggcgtcgc
     1801 ccagtccaag tgggacctct cgacagtctg gccggacggc aacttccaca ccaagaacgc
     1861 cgtgcgcttc gctgccgatg tgaaggccgc gaccaagggt gccgtcgaca tcgccgtcaa
     1921 gtcgggcggc cagctcggct tcaagggtcc ggagcatctg cgcgccgtgc gcgacggcct
     1981 cgtgccgatc gccgatatcc tcaacattca gcagatcggc gacgagcctc tgctcggcat
     2041 cgagagcatc gcgttcctgg tgtcgtcgcc cgccgagctg aagctcctcc acaagcacat
     2101 ccggccgatc tacgagcaga tcgcggcccg caacaaccag aagatcctct acatggtgcc
     2161 gtggccgaca cagtacctgc acctgaaggt caagtcgaac acgctcgacg gcctcaaggg
     2221 cgtgaagatt cgcgtgcccg acaagggtgc ggccgacatg ctgacgctcg tcggcatggc
     2281 gcccgtgctg atcccctggg gtgagacgat cccggcgctc gcctcgggcg tcgttgccgg
     2341 tgtctcgacc tccgccgtct ccggcgtcga cggcaagttc tgggagttcc tgaagtacgt
     2401 ctatccgacc aaccacgtct ggtcgtcgca gatgatgaac gtcaatctcg atgcctggaa
     2461 aaagctctcc ccggagcacc agaaggcgat cgaggacctc gcccgcaagc tcgagcccga
     2521 gttctgggac gtctccgtga aggccgacgc cgacagcctc gccaagctca aggcgcaggg
     2581 catggaggtc gtggagctgc cacctgcaat gatggccgac ctacgggcga agacggcgaa
     2641 tatcttgaac gagtacatga agcgcgtgcc ggcatcgcag ccggccatca aggcctatct
     2701 cgcggaagtg aagcgctgag cgcgcttctc ctgcaggaaa gcgaagggat cgcccatgtc
     2761 catgccaccg cccgcccgcg tgggcgatcc cgtcgccgag atcgatactc cggcgctcgt
     2821 catcgatctc gatgccatgg agcgcaacat cgcccggctc gcggcgttcg cagcgaagac
     2881 cggcgtgaag ctccgcccgc acgctaagac ccacaaatct cctgatatcg gccgccgcca
     2941 gatcgccgcg ggcgccgtcg ggctctgctg ccagaaggtc tccgaggccg aggctatggt
     3001 ggccggcggc atcggcgacg tgctggtctc gaacgaggtg ttcggtgccc gcaagctcga
     3061 ccggctcgcg agcctcagcc gccgcgcgac ggtcgcggtc tgcgccgaca atgtcgaggc
     3121 gatcgaggcg atcgcggcag cggccggacg ccagggcgtg cgcatcacga cgcttgtcga
     3181 gattgatgtc ggtgccaacc gctgcggcgt cgcgcccggc gagcccgccg tggtgctcgc
     3241 ccgcctcatc gcctcgaagc ccaacctgaa gttcggcggc ttgcaggcct atcaggggcg
     3301 cgcgcagcat ctgcgcacga tcgcagagcg gcgtgccgcc atcgaccacg ccatcgaggt
     3361 cacacggcgg acggtcgagc tcctcgaggc cacgggactg ccacccgcga tcgtcggcgg
     3421 cgcaggcacc ggcacctacg atctcgaggg ctcgagcggc gtctacaccg agctgcaggc
     3481 cggctcctac gtcttcatgg atgccgacta cgcccgcaac aagcgcgccg acggccggcc
     3541 ctacgacacc ttcgagcacg cgctcttcgt catcgccacg gtgatgagcc tgcctgtcgc
     3601 cgagcgcgcg gtgatcgacg cgggcctcaa gaccttcgcg acagacgccg gcatgcccga
     3661 gccgcgcggg ctcgacggcg ccagctacat ccgcccgtcc gacgagcacg gcatcataga
     3721 tctcaccaag tcgaaccacc gcctcgcgct cggtgatcgc gtggccctca ttcccggcca
     3781 ctgcgacccg acggtcaacc tccacgactg gtacgtcggc gtgcgcggct acggcacgcc
     3841 caacgcacac gtcgaatgcc tctggcccgt cgccgcccgc ggcgcgattt tctaggccgc
     3901 gccgtcaagc tgcggtgggg cgattggttc ggagccgttg caacgaagtg aaagatttcg
     3961 tggcgcaccg ccgagcgcgc tactgaccct gagcggaagt ggcagggccg gaacttgcgc
     4021 acggccgctg cgtccgctta cgataatcgc gtccaagaag gcgccgacgt tatgcgctcg
     4081 gtgccgcctc gtccgcaacc gaaggtatga cgctttaggg gcagaaaatg gtgtcctcgt
     4141 tacgaaggca agtggaagac tacgttgggc tcctgatttt cacgattgcg atgacggtct
     4201 ggctggcaat ttctgcgtgg gctcaaggtc accagtggcg cttttccctt gagaacatgc
     4261 aagtatctgc tcgggctgac aggaccaatc cttggaagtc ttggggtgtc caggacgatc
     4321 cgtcggctcg gaggcactgt tggggcgaga ttcccgtcat tcagacaccg agggactggg
     4381 gccaatacgc gtactcctgg agcggtatat gtgaaacgag cacccccagg ctgcatgggt
     4441 cgggaacact aaccatgcat cgcctggtga cagggcccaa cattggtact ccttacatcg
     4501 gcgaggcact cttcagcgcc ccggcgtcat tcaatctcgg aaaaattgct gaccagcaga
     4561 actggatggc agctgcagcc cagtacttta cacctttgct tgaaaagcag agggtacgcg
     4621 acaattacgt gtcgccgccg ccaccgcccc gccccgacaa ctcagcgctt gtaatggttg
     4681 gcctgggagc tgctggtgcg cttggcatcc tcttcatcat gttgaagact tcgagaggcg
     4741 gcgttggctc gccttcgatt ctagagaggt ctgctctggc atcaatgatc gcggctttaa
     4801 catcgtcaat cctcgtggca ttcggcgtcg cggacggcgt gaagctagtc actgcgacat
     4861 tggcggcatt cagtgtcggt atcgtggttt cgatggctac ttgaagggcg cggatgatgc
     4921 gagtagtcag cgccattgtc atgttcgccc ttgcgacaat tgcgtacgtt tctcagctgt
     4981 ctgctcaacg gcttgaggaa ctagttccct actacatccg cgcagcgggg gccgactcat
     5041 accggagcag cgtgctgatg cctaaacggt tggtcagtga ccgccgcatc agtcggatcg
     5101 aattggattg gaaaggagga tgggcgcggc agtcctgccc gcccgaagcc gtgagcgtct
     5161 ttgtcgtgac aaggcccgag ttgtgtcggg cagtcgcgac caattcttcg gaccagcagg
     5221 ccgttgtaca cggtactggg ctgatcgaag ttcgcgcttg gccaagaagc ggcaatcccg
     5281 agacgctgtt tcgcgggaat atatcgttca acttggggca aatggtcgga gcccaggggc
     5341 agtctgtatc aggtcaggcg cgggcgttcg ttacccaaat cactgcgaaa ctggaagact
     5401 tcttgttgag gcacgctgcc gccagccaca agattgattt acagaaccaa atagtactga
     5461 cggcgggctt gattgcagct gccgcgttcg gcctgttcct ctacctcaca agcaagggct
     5521 cggccgaagg cgatggcgga cggatactcc gcaagctcct gttctcactc gcaattggag
     5581 ccacaagcgc tgcgattgca gcccacctcg gtcacggtga agacttgaag gtcgccgcgg
     5641 tctccgttgg agcgttcatg gtgacgcttg tggtgtccgc tgcgttttga tccatgtttc
     5701 gtccatggag tttattctga ccgcgctgct gcactcaact gctccatgcg tctatctggc
     5761 caataatacg tgtgtcgtcg tcgggcgctt cgcacacagg gcatgggtaa aggttacctg
     5821 tcgggtagtc gcccttgcaa ttgtcacaaa atatgtggcg gcaattttcg cagacaataa
     5881 tagcagtatt aagatcttct tgcccttcgt atccgcacga attgcagcgc ctgtaagatc
     5941 tcgtactcac ttgtggctcc ctagctagtt tccctcgcgc cagcttctag ttggggccta
     6001 cggccaactt tccgctgttc tagtagggag cagacatagt tcaagtattg gcctacgtcg
     6061 agacgacttc cccttatggc ccatcgcgtc gggcggagcg tgcagcacga tgggtcctcg
     6121 ggacgagccc gaggatgaca gtcgatggcg tggcgactgt gaagtccaac gcccgcacct
     6181 gccccaaatc cgacctcttc cggcgccaag catcagagtc aatcgcgcac gcgatccagc
     6241 gtcgccaacg cgaatagcgc gacctcgtgc cgaggtttcg gacgaactcc cctaccggca
     6301 cctcgccgca ctacgacgtc ggcccctgct ggctcgcccg gatgacgatc ggcgcctcga
     6361 tcaggcggac ggccggctgc ttgccgtcgg ccggcacgag cgctggattg tttgggagcg
     6421 gcgccttgtc cttcgcaggc tcggcccaac cggacgcccg cacgatcctg tcgacgccga
     6481 gcgggtagat cgtcacctgg tcgggctcga gcttcaggcg caggaagttc ttgtagtcgg
     6541 cgatgcggag cgcgccgaag gcgtcctccg tgtgcatgcg ccagagcact ccggtgatga
     6601 cccaatagag gccccagatg aagccgccga ggagcccgcc gagcagcacc atctggaagg
     6661 ggtagaggag gccgatgagc tctcgcgcag ctttcggatc gacgctggtc tgcgtcgcgg
     6721 taatgggttg cagcggcggc agcgcggccc cgggcggcgg aggcacgctc gatacgctcg
     6781 agggcgcgga ccggctgcgg ccttcctcgg cggcgatccg ggtccgggtc tccgtgcggt
     6841 aggtcgagat cggctcaagc gtcttctgca cgaccggcct gacgatcgtc ggcatccagt
     6901 ccgcactgag catcgcggac gcgccgcgcg cggcatgcgg cgcgagccaa ttgttgaagg
     6961 ccgtgaacgt caggaacagg gcgaacgcgg cgatcatgtg cgcgaggaag tgcccgccgc
     7021 cgacaaaggt ccgaaacagc cctgcccgga tgccgcggcg ctcgtgcacc tgcacatagc
     7081 tcaggagcac gaatgccagt ccgaagacca tggcgacgaa gccgatgctg agcgtcgcct
     7141 gcaggatgta gaccggcatc caggcgagct gtgccgggat ggtcgcaaac gagatgtgat
     7201 cgatctcgcc ggacgagatg tcgtgggcgc ggaccaccgt gtggaactgc caggtgatca
     7261 gccagtagat gaggccaatg ccgatgccga aggggatgtt gtagagcggg aagaacaggt
     7321 tcctgaggct cagccaccag ctcgtgctct tgtcgggata gcacttggtg tcgcgctggc
     7381 ggggcgcggt agcagtgcgg gggctgcccg gccggagcgc ctcgcgcacg gcctctcgaa
     7441 cacttccgac cgggccatcg ggccgcttga gccgcgcgct gtactctgca ggcgaccagt
     7501 ctcccgacgg gcccgcgacc gtcggctgcg ggctctgctc gacccactgc acgcgctccg
     7561 gccaggtgat cttgatctcg ttcttcaagg tgtgcgtcgg atgcaggaac gagccgccgc
     7621 ccgccgtgaa gaagtgcaca tcgagatcgg gctggtagta gcggttgtag tggtgccagt
     7681 cgcccgcgac gaccgcgcag atgcgcacgc cgcgctggcg cgcgatcgag gtgatcttga
     7741 agaagttctg ctcctcgtcg ccgccctcga ggtcggcgat catccacgac ggctcggcga
     7801 tgcacaggat gagcttgtcg tccgggccca tctcctcggc gatcttctga aagtagttca
     7861 cctgcgcgtt gtcgagatag ctcgagaact ggatgtcggt gccccagatc caccacttgt
     7921 gcggcagctt cagcgcccag tagctgcgat gctgctggca ctgccagccg ccgatgacgt
     7981 tgccgcgtcc cgacgagagc cggtcccgcg ccgagcagaa caggctgtcg aaggcggtaa
     8041 ggccgtcgta ccagtcgtga ttgccgggaa tggcgaacag cttccgctgc ggctcggaga
     8101 cgttgtaggc ccagccgaac ggcacctgga gccgcttctt gtattcctca cgcgtcgcct
     8161 gcgggtagca ctggtcgccg cccatgacga ggatctcgcc ggcctttagc gtgcccacct
     8221 cgcccatgtc gagcgtcggc ttcgccagca gataggccat cgtgtaggtc gattcgaacc
     8281 cgtcgcccgt gtcggcgaca tagtcgatcc agatggcgcc gttctcgtcc gtcgtgaggc
     8341 gccgctcggg atcggaggag gaggggtcgg aatagtcgta gcgctcgatc agccgcgtga
     8401 cggtgctctg gtcggtcgcg gcctggagga gacgctgatc ggcgtactgg ccgaacaccg
     8461 acgagacgat cgtgcgaacg ccgatgcggg cgagaacgcg cggatcgtac cagctgacca
     8521 ttcccggcag ctcgccgtca cgcagctgct catagggcgt cttgttcgac atggcgccac
     8581 ctcagtgtcc gaagctgaca gaaagccttc accgtgggtc atccacggtg ggtcggcgca
     8641 gtccctcgcg ccggccgcgc tctggccggc gtgcaatccg gcgcgggcct cagctcccct
     8701 tcaggccgtg ggcgttcggg aaaaacaggt ctttccaatc gccgggcttt accttgatca
     8761 ggccgatctt gtgcatgaac tcggcatagc gcatcacgcc cttgggcgtc atcgtgaact
     8821 cgatcgccgg atcgtccagc atggcgagaa tctcctcgac cgtgcttttc ttgtcgcggc
     8881 tgatctcgag atagcgcagc gccgccttcg gcttgccctt gttgatcgtc tcgattgcct
     8941 cgccgagcgc cgccacgaaa gccgcgtaga ccttggggtt ctcgtcatgg aacttcttgg
     9001 tcgtccagag cagattgaaa gtgcctgggc cgccgagcac atcgtaggag ttgaggatgg
     9061 tgcggatgcc gggcgtcttc agctgctgat actggaaggg cggcgaggag aaatgcccag
     9121 tcacctccga ggcgcccgag agcagagccg cctgcgcatc cgggtgcgac atggcgagcg
     9181 tcaatgcatc gaacttggcg tgattgccga cgccgaactc cttctcggcc gccatcatca
     9241 gcgtgatggc ctggatcgac acctttaccg cgggcagcgc gatcctgtct ttctcggtga
     9301 gatccttgag tgacttcgca gcaggattgc gcacattgag gtagatcggc atcgagttca
     9361 tggccgcaac agccttgaca tcgatcgatc ccttggtgcg ggcccacagc gtcgtaagcg
     9421 gcccgacgcc gcccgaggcg aagtgcaggc tgccggagag tatggcgtcg ttcatgggcg
     9481 ccccgcccgc gagcgtccgc cacgtgacct tcaattcgac tccggccgcc ttggcgtgct
     9541 tctcgacgag gccgtcggcc tccatgatca tgagcggcag ataggcgatg ccgaactgct
     9601 tggcgacatt cagttcgccg acctccgcgt ttgcaacgcc tgcagtcatg gcaacggcac
     9661 acgccgctgc tagcgccgaa cggcgcatcg tgctcgacaa cattgcacgg tcctccctga
     9721 gcgacggcct cggctcttgt cggccgtcat agctgtagaa cctgtctagc acggcgagcg
     9781 cctctgctgt cagggcgctg catcgtgcaa aactggctct ttcggtcgca acccactatt
     9841 tcatccaaat gacaggcaag gagcgcggca tgttccgctg gttcgaaacg cgcatcgacc
     9901 cgttccccga ggagccgatc cggcagccgc cggcaacgct gctcgcattc ttctggcact
     9961 atctgaggcc ggttgcgtgg gcattcgcag cgctgaccgc gatgaccctc atcatggccg
    10021 tgatcgaggt cgccatcatg gcgttcatcg gccgtgtggt cgaccttatg aaggaggcga
    10081 agaccccgca cacgttcttc gccgatcacg gcctcgagct cgcgctcatg gccggcatcg
    10141 cgctcatcgt gcgcccgctc gtctcggcgc tcgccgactt caccaagcag cagatgatcc
    10201 aggggcctgt gaacatccgc acgcgctggc tggcgcaccg ctatgtgctc cgccagagca
    10261 tcagctactt ccagaacgac ttcgccggtc gcgtggcgac gcgggtcatt caggtcggcc
    10321 cggccgtacg cgatgtcgcc ggcaacacct gcgacgtcgt cgtgtgggtc gtcgtccact
    10381 ggatcggcgc gttcgcgctg ttcttcgcgg ccgactggcg cctcgtcatc ccgctgacgc
    10441 tgtggctcat cggctacatc atcgcgctca cctacttcgt gccgcgcatc gagcgggcct
    10501 cgcacgacat gtcggaggcg cgctcgatgc tgaccggtcg catcgtcgac agctacacca
    10561 acattgcgac ggtgaagctc tttgcccaca ccgaccacga ggaccgctat gcccgcgagg
    10621 cacagcgcca gttcctcgag cgctacatgg cgcagctccg cctcaacacg ctgatggaga
    10681 tcgtgctctg gtcgctgaac ggcctcgttc tggttgccac gctcggcctt gccgtgtggc
    10741 tgtggacggg caatctcatc accatcggcg ccatcacggt cgtttcgacg ttggtcatcc
    10801 ggctcatgaa catgtccggc tggttcatgt ggacgctcgc cggcatcttc gacaacatcg
    10861 gcgttgtgca ggagagcatg cagacgatcg cgcgtccgct gcaggtcgtg gacagccccg
    10921 gcgccaagcc gatcgacgtg ccgaagggcg cgatccgcta cgaggcgatc ggcttccact
    10981 acgagaaggc cgcggtcgag aacgtggaaa gcgccggccg gaagagcttc tgggacagcc
    11041 gcaaggcggc acgggcgaag gccgatccgg gcgaggagcg cgcggagcct gcgaagctcg
    11101 ccggcatgcc gagcctgccc cggcgctccg tcatcgagcg cctgacgctc gacatcgcgc
    11161 ccggcgagaa ggtcggcctc gtcggtcgct cgggcgcggg caagtcgacg ctcgtcaacc
    11221 tgctgctgcg cttctacgac ctcgagaagg ggcgcatcct gatcgacggg cagaacatcg
    11281 cggaagtgac gcaggacagc ctgcgcgcca acatcggcgt cgtgacgcag gacacctctc
    11341 tcctgcaccg ctctgtgcgc gacaacatcc gctacggccg caccgaggcc accgaggcgg
    11401 agatcgtcga ggcggcgaag cgggccgaag cgcacgagtt catcctcggt ctcgaggatg
    11461 ggcgcgggcg gcgtggctac gacgcccacg tcggcgagcg cggcgtgaag ctttcgggcg
    11521 gccagcgcca gcgcatcgcc atcgcccgtg tcctcctcaa gaacgcgccg atcctggtgc
    11581 tcgacgaggc gacgagcgca ctcgacagcg aggcggaggc agcga
//
LOCUS       DAORNH010000131        11461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4258_length_11461_cov_2.560319, whole genome shotgun sequence.
ACCESSION   DAORNH010000131 DAORNH010000000
VERSION     DAORNH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4258_length_11461_cov_2.560319"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PK264_13715"
     CDS             <1..630
                     /locus_tag="PK264_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76966.1"
                     /translation="LAYKVGETSLPGCPMTWITASARNALLTGLGAAAAILLVWKLVI
                     GWDLLGLLGAVTRFTHVGAVIVWIGMVWFVNFIQIAAIASADDTGRRTLLQGVVPRVA
                     WTFRMASNLTVASGLLLLYFAGYLSRSVVSQGIPPMRHALLWLAVVGGFAMWSFVHHV
                     IWPSLQIVLREGDPAAKAAARERVHTYARINLALAVPVTLVMVAAGHLY"
     gene            638..1495
                     /locus_tag="PK264_13720"
     CDS             638..1495
                     /locus_tag="PK264_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRD76967.1"
                     /translation="MAKATHPSSAELVRERAREGEIDRYLAATRSPAAARADLITLAA
                     FAAEIARIPATVREPMMGAIRLQWWRDAIALPAGEASGHPVANAIRETITRHRLPPAL
                     LIGTIDAREGDVTGNLLPDARSRRAWLAKREGALFELAWRILGLPPSTEADEACADAG
                     HAYGLARALSHLPAAIQHGGLLLSGEELSAAGVDVERLTSADAAAQLEPLIEALRVEA
                     ERAVGRARELIRQLPRGAALALLPAASVTGYLAAQRRGPRPSLHAITEPLPLARVTRI
                     WLASVTGRL"
     gene            complement(1514..2761)
                     /locus_tag="PK264_13725"
     CDS             complement(1514..2761)
                     /locus_tag="PK264_13725"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRD76968.1"
                     /translation="MPTSFELLSERAADAPLPEVAVPVKMWTRGVPVDAASIGQLART
                     AGMPFIYKWLAVMPDVHYGKGSTIGSVIPTIGAIIPAAVGVDIGCGMMAVRTSLGAGH
                     LPDSLAKVRSALEAAVPHGRVAQKKKRDPGAWGEPPDAAAEAWRQLGPGFEVIADKHP
                     VLRKANTIVHLGTLGTGNHFLEVCLDEADSVWVMLHSGSRGVGNAIGSYFIERARERI
                     IAEGIDIQDRDLAWLAEGSPLFADYVEAVEWAQTFAMLNRQIMMRHAIEALRRVLHRR
                     FEAHLEAINCHHNYVAREEHFGQHCFVTRKGAVRAAEGVMGIIPGSMGAKSFIVRGKG
                     NLESFCSCSHGAGRVMSRGEAKRRFTLEDHARDTAGVECRKDAAVIDETPKAYKGIDA
                     VMAAQSDLVEIVHTLRQVVCVKG"
     gene            complement(2801..>4149)
                     /locus_tag="PK264_13730"
     CDS             complement(2801..>4149)
                     /locus_tag="PK264_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76969.1"
                     /translation="LHICDTTTDLLYKLEAVRLLCRETGCAQRYLTHDGLNALYQATK
                     RIDANHKADRHARFLAYLHRVQDEDLTLAIAMTDAKGDRSKRPGAQSNRDVYVHVKER
                     RPGGIVIRGTKAIVTAGPYVHELLVMPCRTMTEDDKDFALCCAVPVDAPGVTIISRPA
                     GRPGEAAARFSAKYGQSTAVVQFDDVFVPHERVFVDGEFEEGGFLTTSYATHHRHSCI
                     GARAGFGDLLIGAGALMIEANGLDPERHGHIRDAMVELIKIVEGFFACGVAASVYGQK
                     DPDGSIMPDTVFSNIGKLLLATQIFDMHRLANYVSGGLVVALPGPDEDHNPETAASLS
                     AVLAGRPDVPASARLEVARLMEDLTASYTGGWMSAISLHGGGSPEAMKREIWRNYPVG
                     EKAEVVDQLLGRGVLADGRRVSKQPGRCCATGCEVPEIAAPRVIGSDRNDKKDAAE"
     assembly_gap    4150..4164
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4165..4361)
                     /locus_tag="PK264_13735"
     CDS             complement(<4165..4361)
                     /locus_tag="PK264_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HRD76970.1"
                     /translation="MMMSSVEFRESLRRLRPRVFVNGRRVESVADEPLLAPGVAAIGV
                     TYDMALREEHKLLMTARQGTSG"
     gene            complement(4517..5869)
                     /locus_tag="PK264_13740"
     CDS             complement(4517..5869)
                     /locus_tag="PK264_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrocarbon degradation protein"
                     /protein_id="HRD76971.1"
                     /translation="MGTALRLATAGILTATAILASNGSAEATEGYFRNAYGARHGGLA
                     GAGVADGRDATAAALNPAGIIRTSTELVIAATVFSPRREYDHISGAGFLPAFDIESDK
                     NYFLIPNIAIGWRTPGLGFADAVALTMYGNGGMNTTYPATSGGAFCPFPGMGPFCGGR
                     AGVNLEQMMLSLAFAKQVAPGVSVGIAPILMAQKFDADGLAAFGGLSSDPAHLTNLRP
                     DWAFGGGLRAGVEWTVMPGVRVAVAGTTPIWSQKFDGYRGLFAEGGSFDIPASIQAGF
                     AVDMTKNVTLMADWRRIFYSGVPAINNPSTNIGNCALGQPQFCLGASSGAGFGWEDIN
                     VYKVGLEMRHVMPNLDLRFGYAFAEQPYGSRDVMFGTLAPGIVQHHFTAGGLLHEVLP
                     NLDLEFAAAYVPAARVTGFELPLVAAGTGHTIASEMYQFEVTMGFKYRFGGPAHEPLK
                     "
     gene            complement(6224..6727)
                     /locus_tag="PK264_13745"
     CDS             complement(6224..6727)
                     /locus_tag="PK264_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRD76972.1"
                     /translation="MSDASAAPNSKSMSIIVTKGTLDWAYPPFILATTAAAMDVKVTM
                     FFTFYGLPLLKKKLDLQISPLGKPDMAMPMMGMHMKMPNLVAMLPGVNAGATTMMKNL
                     IKKKGVASIEELRSTAVEAEVELIACQMTMDLFEFKTSDMIDGISLGGAATYMENALK
                     SDINLFI"
     gene            complement(6771..7109)
                     /locus_tag="PK264_13750"
     CDS             complement(6771..7109)
                     /locus_tag="PK264_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76973.1"
                     /translation="MKQQMIDMMAMMMPYMRPLAQAAGAAALFAIAARLIGQTSIARL
                     LAALVLVIGVFFIACEVAGRFLGFEPTILFAAPADRALYRNQWPFWCIGLTGLVIGLL
                     IRAGTSSKSA"
     gene            complement(7205..7588)
                     /locus_tag="PK264_13755"
     CDS             complement(7205..7588)
                     /locus_tag="PK264_13755"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HRD76974.1"
                     /translation="MRQRSKITLAGLAVLALGATGAMAVELKDMIGRWKWTDYTVEVK
                     ECTTNPSGAGFCATVVDGPKNKGMEMMRSKLEKKGEAIVGKVAHPATGDIYDTKMTYD
                     GKDKWTMDGCTEKGVCAKGDFVRVK"
     gene            7782..>8402
                     /locus_tag="PK264_13760"
     CDS             7782..>8402
                     /locus_tag="PK264_13760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663939.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRD76975.1"
                     /translation="MPETDIRNFNINFGPQHPAAHGVLRLVLELDGEVVKRVDPHIGL
                     LHRGTEKLLEHKTYLQAIGYFDRLDYVAPMNQEHAFCLAAEKLLGLSVPKRGQLIRVL
                     FSEIGRILSHILNVTTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMHAKYFRIGG
                     VHQDLPRKLIEDILAWCKAYPKVIDDIEGLLTNNRIFKQRNVDIGVV"
     assembly_gap    8403..8444
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8445..8981
                     /locus_tag="PK264_13765"
     CDS             <8445..8981
                     /locus_tag="PK264_13765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663939.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HRD76976.1"
                     /translation="GVMVRGSGAAWDLRRAQPYECYNELAFDIPIGKNGDCYDRYCLR
                     VEEMRQSVKIMGQCCQKLLSAEGEGPVSADDKKVVPPKRGEMKRSMEALIHHFKLYTE
                     GFHVPAGEVYACVEAPKGEFGVYLVADGSNKPYRCKIRAPGFPHLQAMDFMNRGHMLA
                     DVSAILGSLDIVFGEIDR"
     gene            complement(9098..9172)
                     /locus_tag="PK264_13770"
     tRNA            complement(9098..9172)
                     /locus_tag="PK264_13770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9138..9140),aa:Gln,seq:ttg)
     gene            complement(9295..9678)
                     /locus_tag="PK264_13775"
     CDS             complement(9295..9678)
                     /locus_tag="PK264_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76977.1"
                     /translation="MTTMLLLASALTIALGIAHSWIGEVRLIGPLLDAGTRSGILARS
                     RFARQVLRFAWHITSLAWLGFAGMLIGFAIWPDADARRIALAVIGATMLLSGLIVLVM
                     SRGRHLAWPLFLAIAGICAAATVQR"
     gene            9859..10410
                     /locus_tag="PK264_13780"
     CDS             9859..10410
                     /locus_tag="PK264_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HRD76978.1"
                     /translation="MADRLEPDAWINAGLRALRSGGVEAVRVEKLAATLGVTKGSFYW
                     HFKDRPALLQALLQGWRERATSSVIAAVEEGGGDARLLLRRLLAIVTADDGRLERAIR
                     AWGDADPAVGRAVAKIDQERMAYVEALFRKLGFGERVARDRARFAYQALVGQYALSRR
                     VRSADEREHRIELDEIVAMLLAR"
     gene            10608..>11461
                     /locus_tag="PK264_13785"
     CDS             10608..>11461
                     /locus_tag="PK264_13785"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HRD76979.1"
                     /translation="MRLPILLAPMAGACPPALSAAVATAGGMGACGALLMQPAAIKSW
                     AGELRAKTNGAFQLNLWIPDPPPARDPVREAAVRGFLSGFGPEVPATAGDTRLIDFDA
                     QCEAILEVGPAVVSSIMGVYPPAFVARLKERGIRWFATATTVSEAIAAEVAGADAIIA
                     QGMEAGGHRGSFDATRPDGHLVGLFALLPAVADAVKVPVIATGGIGDARGIAAALLLG
                     ASAVQIGTGFLRSTEAAIAPVWADAIGRAAPEDTIATRAFSGRLGRSIATEYARAASA
                     PEAPAPAP"
BASE COUNT         1874 a         3800 c         3759 g         1971 t
ORIGIN      
        1 ctcgcctata aggtcggcga aacctctttg cccggatgcc cgatgacctg gatcacggcc
       61 agcgcccgca atgcccttct caccggtctt ggtgcggcgg cggcgatcct gctcgtctgg
      121 aagctggtga tcggctggga cctgctcgga ctgctcggcg ccgtgacgcg gttcactcac
      181 gtcggcgctg tgatcgtttg gatcggcatg gtctggttcg tgaatttcat ccagatcgcc
      241 gcgatcgcct ctgccgatga caccgggcgg cgcactcttc tccaaggcgt ggtgccgcgc
      301 gtcgcctgga cgttccggat ggcgtcgaac ctgacggtcg cgtcgggcct gctgctcctg
      361 tatttcgcag gctatctctc gcgctccgtc gtgagccagg gcatcccgcc gatgcggcat
      421 gcactcctct ggctcgcggt cgtcgggggc ttcgcaatgt ggtcgttcgt ccatcacgtg
      481 atctggccat cgctccagat cgtgctccgg gagggcgatc cggcggccaa agccgcggcg
      541 cgagagcgcg ttcacaccta tgcgcgcatc aatctggcgc tggccgtacc cgtgacgctc
      601 gtcatggtgg cggcgggcca cctctattga gggccgcgtg gcgaaggcga cgcatccgag
      661 cagcgccgag ctggtccgcg agcgggcgcg cgaaggcgag atcgatcgct atctcgcggc
      721 aacgagatcc ccggcagccg cgcgggccga tctcatcacg cttgccgctt ttgcggccga
      781 gatcgcgcgc atccccgcga ccgtgcgcga gcccatgatg ggcgcgatcc gcctgcagtg
      841 gtggcgcgac gccattgctc tgccggcggg cgaggcgagc gggcacccgg tcgccaatgc
      901 gatccgcgag accatcaccc gccaccgtct gccgccggcc ttgctgatcg gcacgatcga
      961 tgcgcgcgaa ggcgacgtca ctggcaacct gttgcctgac gcgcgatccc gtcgcgcctg
     1021 gctcgccaag cgcgaagggg ccctctttga gctcgcctgg cgcatcctcg gcttgccgcc
     1081 ctcgaccgag gccgacgaag cctgtgccga cgccggtcat gcctacggtc tcgcccgcgc
     1141 gctctcgcac ctgccggcgg cgatccagca cggcggtctg ttgctttcgg gcgaggaact
     1201 ctctgcggcc ggagtcgatg tcgagagact gacgagcgcg gacgctgcag cccagctcga
     1261 gccgttgatc gaggcgctca gggtcgaggc tgagcgcgcg gttggacgtg cccgcgaact
     1321 catcagacag ctgccgcgcg gcgcggcact cgcactgctt cccgcggcat cggtcaccgg
     1381 ctatctcgcc gcgcagcgcc gcggaccgcg tccgagcctg cacgcgatca cggagccgct
     1441 gccgctcgcg cgcgtcaccc ggatctggct cgcgagcgtg accgggcggc tctgacggcg
     1501 tggatcggtg gcctcatccc ttcacgcaca caacctgtcg cagcgtgtgc acgatctcga
     1561 cgaggtccga ctgcgccgcc atcacggcgt cgatgccctt gtaggccttg ggcgtctcat
     1621 cgatgacggc ggcgtccttc cggcactcga cgccggcggt atcgcgcgcg tgatcctcga
     1681 gcgtgaagcg ccgcttcgcc tcgcctcggc tcatgacgcg gccggcgccg tggctgcatg
     1741 agcagaagct ctcgagattg cccttgccgc gcacgatgaa gctcttcgca cccatcgagc
     1801 ccgggatgat gcccatcacg ccttcggccg cgcgcaccgc gcctttcctc gtcacgaagc
     1861 agtgctggcc gaaatgctct tcccgcgcca cgtagttatg atggcagttg atcgcctcga
     1921 ggtgcgcctc gaagcgccgg tggaggacgc gccgcagtgc ctctatcgcg tggcgcatca
     1981 tgatttggcg gttcagcatg gcgaaggtct gcgcccactc gaccgcctcg acgtagtcgg
     2041 cgaacagcgg gctcccctcg gcgagccagg cgagatcgcg atcctggatg tcgatccctt
     2101 ccgcaatgat gcgctcccgg gcgcgctcga tgaagtagct gccgatcgca ttgccgacac
     2161 cgcgggagcc cgaatgcagc atcacccaca cgctgtccgc ctcgtcgagg cagacctcga
     2221 ggaagtggtt gccggtgccg agcgtgccga gatgtacgat ggtgttcgcc ttgcgcagca
     2281 cgggatgctt gtcggcgatc acctcgaagc ccggtccgag ctgccgccac gcctcggcgg
     2341 cagcgtcggg cggttcgccc caggcacccg gatcgcgctt cttcttctgc gcgacacggc
     2401 cgtgcgggac ggcggcttcg agcgccgagc gcaccttggc gaggctgtcc ggcagatggc
     2461 ccgcgccgag cgatgtcctc accgccatca tgccgcagcc gatgtcgacg ccaacggccg
     2521 ccgggatgat cgcgccgatg gtcgggatca ccgagccgat ggtcgagccc ttgccgtagt
     2581 gcacgtccgg catcacggcg agccacttgt agatgaaggg catgccggcg gtgcgcgcga
     2641 gctggccgat cgaggccgca tcgaccggca caccgcgcgt ccacatcttc accggtacgg
     2701 ccacctcggg aagaggcgcg tccgccgcac gctcgctcag gagctcgaat gaggtcggca
     2761 tgatcgatct gacttgctga tgcgaacagg gaagagggcc ctactccgcc gcgtccttct
     2821 tgtcattgcg atccgatccg atcacgcgtg gggctgcgat ctccggcacc tcgcaaccgg
     2881 tggcgcagca gcggccgggc tgcttcgaga cgcggcggcc atcggcgagg acgccgcggc
     2941 cgagcagttg gtcgacgacc tcggccttct cgccgacggg atagttgcgc cagatctcgc
     3001 gtttcatggc ctcgggcgag ccgccgccat gcagcgagat cgccgacatc caccctcctg
     3061 tgtaggaggc cgtgagatcc tccatgagac gggcgacttc gagccgcgcg gatgccggca
     3121 catcgggccg gcccgcgagc acggccgaga gcgaggctgc cgtttccgga ttgtggtcct
     3181 cgtccgggcc gggcagcgcc acgacgagcc cgcccgagac gtagttggca aggcggtgca
     3241 tgtcgaagat ctgtgtcgcc agcagcaact tgccgatgtt cgagaacacc gtgtccggca
     3301 tgatcgagcc gtccgggtcc ttctggccgt agacggaggc cgcgacaccg caggcgaaga
     3361 acccctcgac gatcttgatc agctccacca tggcatctcg gatgtggcca tgccgctcgg
     3421 ggtcgaggcc gttggcctcg atcatgagcg cgccggcgcc gatcagcagg tcgccgaagc
     3481 cggccctggc gccgatgcag gaatggcgat ggtgcgtcgc gtagctcgtg gtgaggaagc
     3541 cgccttcctc gaactcgccg tcgacgaaga cgcgctcgtg cggcacgaac acgtcgtcga
     3601 actgcacgac cgcggttgat tgaccgtact tcgccgagaa cctggcggcg gcctctccag
     3661 gccgtcccgc ggggcgcgag atgatcgtga cgcccggcgc gtcgacaggc accgcacagc
     3721 agagggcaaa gtccttgtcg tcctccgtca tggtgcggca gggcatgaca agcagctcgt
     3781 gcacgtaggg gccagcagtc acgatcgctt tggtaccacg gatgacgatg ccgccggggc
     3841 ggcgctcctt cacgtgcaca tagacatccc ggttcgactg cgcgcccggc cgcttcgagc
     3901 ggtcaccctt ggcatccgtc atggcgatgg cgagtgtgag gtcctcgtcc tgcacgcggt
     3961 gcagataggc caggaagcgc gcgtgccggt cggccttgtg attggcgtcg atgcgctttg
     4021 tcgcctggta gagcgcgttt aggccgtcgt gcgtgagata gcgctgcgcg caacccgtct
     4081 cacggcaaag aagacgcacc gcctcgagct tgtagaggag gtcggttgtg gtgtcgcaga
     4141 tgtgcagcan nnnnnnnnnn nnnncccgac gtcccttgcc gcgcggtcat gaggagcttg
     4201 tgctcctcgc ggagcgccat gtcgtacgtg acgccgatcg ccgccacgcc cggtgcgagc
     4261 agcggctcgt cggcaacact ctcgacgcgc cgtccgttca cgaagacacg cggcctcagg
     4321 cggcggaggc tctcgcggaa ctcgacagag gacatcatca ttgcggaacc ccaattgatc
     4381 tcaggctcga cccgggacgt tatggattcc gcgattgcgg cgcgcaagac acacgacact
     4441 gcacgcgaca aggcaaaggg ggcactcggc cccctttgtc agtttactgt atttttcctt
     4501 cgagccttgt ggcggctcat ttgagcggct catgcgccgg gccgccaaag cggtacttga
     4561 agcccatggt gacctcgaat tggtacatct cgctggcaat ggtatggccg gttccggcgg
     4621 ctacaagagg cagttcgaag cccgtgacgc gtgcggcggg cacataagcc gcggcgaact
     4681 cgagatcgag gttcggcaac acctcatgca ggagaccgcc tgcagtgaag tgatgctgca
     4741 cgatgccagg cgcgagagta ccgaacatca cgtcgcggct gccgtagggc tgctcggcaa
     4801 aggcatagcc gaaacggagg tcgaggttcg gcatgacgtg ccgcatctcg agaccgacct
     4861 tgtagacatt gatgtcctcc caaccgaatc cggcgcctga cgaggcaccg aggcagaact
     4921 gcggctggcc gagcgcgcag ttcccgatgt tggtggaggg attgttgatc gccggcacgc
     4981 ccgagtagaa gatgcggcgc cagtcggcca tcagcgtgac attcttcgtc atgtcgacag
     5041 caaagccggc ctggatggat gccgggatgt cgaacgagcc gccctcggcg aagagaccgc
     5101 ggtagccgtc gaacttctgc gaccagatcg gcgttgtgcc ggcgacggcg acgcgaacgc
     5161 cgggcatgac cgtccactcg acgccggccc ttaggccacc gccgaaagcc cagtccgggc
     5221 gcagattggt gaggtgcgcc ggatcggagg agaggccgcc gaaggccgcg agcccgtcgg
     5281 catcgaattt ctgtgccatc aggatcggtg caatgccaac cgagacgcca ggagccacct
     5341 gcttggcgaa ggcgagcgac agcatcatct gctcgagatt gacgccggcg cggccaccgc
     5401 agaatggccc catgccgggg aacggacaga aggcgccacc gctcgtcgca ggatacgtcg
     5461 tgttcatgcc gccattgccg tacatcgtca gcgcgaccgc gtccgcgaag ccgagcccag
     5521 gcgtgcgcca gccgatggcg atgttgggga tcaggaagta gttcttatcg ctttcgatgt
     5581 cgaaggcagg caggaatccg gccccgctga tgtgatcgta ctcgcggcgc gggctgaaga
     5641 ccgttgccgc aatcacgagt tcggttgacg tacggatgat gccggcaggg ttgagagccg
     5701 ccgctgtggc atcgcgcccg tcggcaacac cggcgccggc aaggccaccg tggcgcgcgc
     5761 cataggcgtt gcggaagtag ccctcggtcg cctcggcgga gccatttgat gccaagatcg
     5821 ccgtcgccgt cagtatgccc gccgttgcga gccggagtgc cgttcccatc tgcccgtctc
     5881 ctccacctgc ggcgcgcagc aggtgggtca tcccgcccgg ccacgccgcc cgctgcccca
     5941 tgccgacttc gggttgctgt cccgaggctt cgtgttcttg gcgacgtgcc gcgcttggcg
     6001 gacgcgcaaa ttcaaaaatg cgaatattcg actctaagaa tattgtcaga tgttcggagc
     6061 gttgactgta gccaatgggg cacattggcg ccgattcgcc ggccttccta ttcgatgagc
     6121 gatggacgca gtacggcgcg cggcaccagg cgcgccggca gcggtacgcg cgagtgatcc
     6181 gcggccctca cggcgcgaca agcgctcggt ctacgccaga gcctcagatg aacaggttga
     6241 tgtcggactt cagcgcgttt tccatgtagg tcgcggcgcc accgagactg atgccgtcga
     6301 tcatatcgct ggtcttgaac tcgaagaggt ccattgtcat ctggcaggcg atcagctcga
     6361 cctcggcttc aacggccgtg cttctcaact cttcgatcga ggcgacgccc ttcttcttga
     6421 tgaggttctt catcattgtc gtcgcaccgg cgttgacgcc cggaagcatg gcaacgaggt
     6481 tcggcatctt catgtgcatg cccatcatcg gcatggccat gtccggcttg ccgagcggcg
     6541 aaatctgcag gtcgagcttc ttcttcagga gcggcaggcc ataaaacgtg aagaacatcg
     6601 tcaccttgac atccatggca gcggccgtgg tcgccaggat gaacggcggg taggcccagt
     6661 cgagcgtacc cttggtgacg atgatggaca tgctcttgga attcggcgca gcagatgcat
     6721 cagacaaggg aacctccatt cataaacaat tggatgcgcg gccgagcccc tcaggccgac
     6781 ttggacgatg tgcctgccct gataagcaga ccgatcacca gcccggtgag cccgatgcac
     6841 cagaacggcc attgattgcg gtagagcgcc cgatccgccg gcgcggcgaa gaggatagtc
     6901 ggttcgaagc cgaggaagcg cccggcgacc tcgcaggcga tgaagaacac gccaatcacc
     6961 agtacgagcg ctgccagaag tcgggcaatc gatgtctggc cgatcaggcg ggccgcgatg
     7021 gcaaacaggg ctgcagcgcc tgccgcctga gcgagcggcc gcatgtaggg catcatcatc
     7081 gccatcatat cgatcatctg ctgcttcatc ggagcctcct gaggcatcag gcagacggcg
     7141 cgcaggttcc agatatcgga gccgtcggga cgcgccgccg ccctaggact ttccaggtcg
     7201 cgagctactt tacgcggacg aaatcgccct tggcgcacac gcccttctcc gtgcagccat
     7261 ccatggtcca cttgtccttg ccgtcatagg tcatcttggt atcgtagatg tcgcctgtcg
     7321 ccggatgagc gaccttgccg acgatcgcct cacccttctt ctcgagcttg gagcgcatca
     7381 tctccatgcc cttgttcttc ggcccgtcca cgactgtcgc gcagaacccc gcgcccgacg
     7441 gattggtcgt gcactccttc acctcgacgg tgtagtcggt ccacttccag cggccgatca
     7501 tgtccttgag ctccacggcc atcgcacctg tcgcgccgag cgccagaacc gccaatcctg
     7561 cgagtgtgat cttgcttcgc tgccgcatgc gtcactccct gagaacggag cccgaattcg
     7621 gtgtcggcca cgcgcctgcg atgctcacgg gccctacatt cgaacattcg caagcattaa
     7681 tatgattaat tggggcgggc gccacttgtc aaagcacatc gccttgcgta tcggcctaag
     7741 tgagagctga gtgagcggac tgaccgccaa ggaccgatgc gatgcccgag accgacatca
     7801 ggaacttcaa catcaatttc ggtccccagc atccggcggc acacggcgtg ctgcgacttg
     7861 tgctcgagct cgacggcgag gtcgtgaagc gcgtcgaccc gcacatcggc ctccttcacc
     7921 gcggcaccga gaagctgctg gagcacaaga cttatctgca ggccatcggc tatttcgacc
     7981 ggctcgacta tgttgcgccg atgaaccagg agcacgcctt ctgccttgcc gccgagaagc
     8041 tgcttgggct cagcgtgccg aagcgcggcc agctcatccg ggtgctgttc tccgagatcg
     8101 gccgcatcct ttcgcacatc ctgaacgtca cgacgcaggc gatggacgtc ggcgcgctga
     8161 caccgccgct ctggggtttc gaggagcgcg agaagctcat ggtcttctac gagcgcgcct
     8221 cgggaagccg catgcacgca aagtatttcc gcatcggcgg cgtgcaccag gacctgccgc
     8281 ggaagctgat cgaggacatc ctcgcctggt gcaaggccta tccgaaggtc atcgacgaca
     8341 tcgaggggct gctcaccaac aaccgcatct tcaagcagcg caatgtggat atcggcgtag
     8401 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcgtc atggtgcgcg
     8461 gctccggcgc cgcctgggac ctgcgccgcg cgcagcccta cgagtgctac aacgagctcg
     8521 ccttcgatat ccccattggc aagaacggcg actgctacga tcgctactgc ctgcgcgtcg
     8581 aggagatgcg ccaatcggtc aagatcatgg ggcagtgctg ccagaagctg ctctcggccg
     8641 agggcgaggg gccggtctcc gcggacgaca agaaggtcgt gccgccgaaa cgcggcgaga
     8701 tgaagcgctc gatggaggcg ctgatccacc acttcaagct ctacaccgaa ggcttccacg
     8761 tgccggcggg cgaggtctat gcctgcgtcg aggcgccgaa aggagaattc ggcgtctatc
     8821 tcgtcgctga cggctcgaac aagccctacc gctgcaagat ccgcgcaccc ggcttcccgc
     8881 atctccaggc gatggatttc atgaaccgcg gccacatgct cgccgacgtc tccgcgatcc
     8941 tcggctcgct cgacatcgtg ttcggcgaga tcgacaggta gcgtcgtagc ggtgcggccg
     9001 caggcggaca ggcgccgatc gggacgccgc ccgttcatgt cgtcggccct tgagcatccg
     9061 gcggacgccg tagcggccca gggtttgcct cagttgctgg ctggggcgcc aggattcgaa
     9121 cctgggaatg gcggaatcaa aatccgctgc cttaccactt ggcgacgccc caatgcctcg
     9181 gccggggccg agagcgggcg gacactagcg gcttcgatcc tgatcggcaa tcgtggaaat
     9241 cgaacccgga attcgagcca agcaagctga tcgagccggg cgccgcggcc gtgctcagcg
     9301 ctggacggtg gcggcagcgc aaatgccggc gatcgcgagg aaaaggggcc aggcgagatg
     9361 ccggccgcgc gacatcacga ggacgatgag gccgctcagc agcatcgtcg ccccgatcac
     9421 ggcaagcgca atgcgccggg cgtcggcgtc cggccagatc gcgaagccga tcagcatgcc
     9481 ggcgaagccc aaccaggcaa gcgacgtgat gtgccaggcg aagcgcagca cctgccgcgc
     9541 gaaccggctt cgcgccagga tgccgctccg cgtcccggca tcgagcagcg ggccgatgag
     9601 gcgcacctcg ccgatccagg aatgggcgat gccgagggcg atggtcagcg ccgaggcaag
     9661 cagtagcatc gttgtcatgc gcgctccttg ggttgcgctg tcgctgtgcc gtccgcgtcc
     9721 cggcgacctt gtccgccccg gcgctcgact gcgcaccggg gaggcttcgc tcaggggcga
     9781 ctgcaccata cgcttgcgta tggtactgtt ggtgccccat cgatgtcgcc ctgtcaatac
     9841 gcaagcgtat ggtgagccat ggccgaccga ctcgagcccg atgcctggat caatgccgga
     9901 ctgagggcgc tcaggtcggg cggcgtcgag gccgttcgcg tcgagaagct cgccgcgacc
     9961 ctcggcgtga cgaaggggag cttctactgg catttcaagg atcggccggc gttgctgcag
    10021 gccctccttc aggggtggcg cgagcgtgcg acgagcagcg tcatcgccgc cgtcgaggaa
    10081 ggtggaggcg atgcgcgcct gcttctgcgc cggctgctcg cgatcgtgac ggccgacgac
    10141 ggccggctcg agcgcgcgat cagggcctgg ggagacgccg atcccgctgt cgggcgcgcg
    10201 gtggcgaaga tcgaccagga gcggatggcg tacgtcgagg cgcttttccg taaactcgga
    10261 tttggcgagc gggtcgcacg tgaccgcgcc cgcttcgcct atcaagcgct ggtcggccag
    10321 tatgccttga gccgccgggt gcgaagcgcg gacgagcggg agcatcgcat cgagctcgat
    10381 gagatcgtcg ccatgctgct ggcgcggtga ggcccatacg aagccacgac atgcttcttc
    10441 ctgtcgtggc ttcgtaccgc gcgcgggtgg tcgcgcttgc aattgacccc cgcgcgtgag
    10501 ccgctacact tgtctccctg ccgacccgat cgcttggagc caggatcgcg atgccgtctc
    10561 gtcacccagc cttgacgcgc gcgacggcat tctgcgacgc atacgacttg cgcctgccca
    10621 tcctgctcgc gcctatggcc ggtgcctgcc cgcctgcgct ctcggccgcc gttgccacgg
    10681 ccggcggcat gggggcctgc ggcgcgcttc tcatgcagcc cgcggcaatc aagtcctggg
    10741 ccggcgagct ccgtgcgaag accaacggag ccttccagtt gaacctctgg attccggatc
    10801 cgccgcccgc gcgtgatccg gtccgcgagg ctgccgtacg tgggtttctc tcgggcttcg
    10861 gccccgaggt tcctgccacg gccggcgata cgcggctcat cgatttcgat gcccagtgcg
    10921 aggccattct cgaggtaggc cccgccgtcg tctcctcgat catgggtgtc tatccgccgg
    10981 ccttcgtcgc ccgcctcaag gagcgtggca tccgctggtt cgcgaccgcg acgaccgttt
    11041 ccgaggcgat cgccgctgag gtggccggcg ctgacgcgat catcgcacaa ggcatggagg
    11101 cgggtgggca ccgcggctcg ttcgacgcaa ccaggccgga cggccacctc gtcggcctgt
    11161 tcgcgctgct gccggccgtc gccgatgcgg tcaaggttcc ggtgatcgcg accggcggca
    11221 tcggtgacgc acgcggcatc gcagcggcac tgctgctcgg cgcgtccgcc gtgcagattg
    11281 gcacgggctt cctgagatcg accgaagcgg ccattgcacc tgtctgggcc gatgcgatcg
    11341 gccgggccgc gcccgaggac accatcgcga cgcgcgcctt ttccggccgg ctcggacgct
    11401 ccattgcaac cgagtacgcc cgcgccgcca gcgctcccga ggctcctgcg ccggcgccct
    11461 a
//
LOCUS       DAORNH010000132        11048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4438_length_11048_cov_2.621759, whole genome shotgun sequence.
ACCESSION   DAORNH010000132 DAORNH010000000
VERSION     DAORNH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4438_length_11048_cov_2.621759"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    119..155
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<156..1032)
                     /locus_tag="PK264_13790"
     CDS             complement(<156..1032)
                     /locus_tag="PK264_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HRD76980.1"
                     /translation="MSVRLGSGEHTYRVVENWAKLPDGWEFMDVGSVACDSRDRVYVF
                     NRGEHPMIVFDRDGNFIKSWGEGKFPRAHGLHIDENDHLYCTDDGGHFVRKCTTDGKV
                     LLEIGVPGKPAPFMSGKPFHRCTHTALSPKGEIYVSDGYGNSCVHKFSPDGKLLKTWG
                     ETGSGPGQFNLVHNIVTDKDGWVYVADRENHRVQVFDGNGRFETEWHNLHRPCGLYCS
                     CPPGAKSPTFIVGELGPTQPVNRAYKNLGPRLSIVDAKGRLVARLGGEDGPGTETGRF
                     IAPHGLAMDSHGDIYV"
     assembly_gap    1226..1235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1294..2232
                     /locus_tag="PK264_13795"
     CDS             1294..2232
                     /locus_tag="PK264_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471258.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HRD76981.1"
                     /translation="MEVFLQQVINGITLGSIYGLIAIGYTMVFGIIGMVNFAHGDVFM
                     LATFFVLILLLLIIQAIGAAGQVLAGATLVIALLLVLVGAMLLTSLWNWVIERLAYRP
                     LRGSFRLAPLISAIGMSIFLVNFVQLVQGPRNKALPPMMRETINLKSLIGYDVTLSHK
                     QISIILITGILLAIFWYLVQHTSLGRAQRACEQDGKMAALLGVNVDRTISLTFVIAAA
                     LAAVAGVMYFIQYGVVKYDDGFVPGVKAFTAAVLGGIGSLPGAVLGGLLIGLIEVLWS
                     QYVSIDYKDVAAFSILAITLIFLPSGLLGRPEVEKV"
     gene            2238..>2694
                     /locus_tag="PK264_13800"
     CDS             2238..>2694
                     /locus_tag="PK264_13800"
                     /inference="COORDINATES: protein motif:HMM:NF023290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3382 domain-containing protein"
                     /protein_id="HRD76982.1"
                     /translation="MADTTTETSSVDFGKALREALITAALTLGLAVPIVLLRTEQDMN
                     NTLILQWRWGLVAALVGIAFAARLGWELVGPHLRGGGSSGKAQRHAAMPRSAQTALAI
                     FGVALLVAYPIFVLWRLGPSGSIKWIDNFGIQILIYVMLGWGLNIVVGLA"
     assembly_gap    2695..2791
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2792..3673
                     /locus_tag="PK264_13805"
     CDS             <2792..3673
                     /locus_tag="PK264_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD76983.1"
                     /translation="VGNLLGPNFWPLWAFWICLPVAGILAAFWGVILGFPVLRLRGDY
                     LAIVTLAFGEIIRLILINWVPVTGGYQGIGHTQPIPRPSFFGIPFNASDEGFSARFGL
                     EFSPIYRTIFLFYVIAALALLTNWVTLRLRRLPVGRAWEALREDEIACRSLGINTTNT
                     KLTAFAIGAGFGGIAGSFFAARQAFISPESFTFIESALILWIVVLGGMGSQIGVAIAA
                     VIMIGGTELLRELDYLKVLFGKDFDPTHYRMLLFGLAGVLLVRWKPRGLVATRSPTIV
                     LKERRSISADLVKEGHG"
     gene            3673..4563
                     /locus_tag="PK264_13810"
     CDS             3673..4563
                     /locus_tag="PK264_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76984.1"
                     /translation="MASAPLLTVEHMTMRFGGLVAINDLSFTAKRGEITAIIGPNGAG
                     KTTVFNCITGFYKPTVGRMALAISAAEAGAMEALTKTGRRFDAASGIFLLERMPDFEI
                     AQRAHIARTFQNIRLFSGLTVLENLIVAQHNRLMSASGWTIKGLLGSRRYKTAELAAV
                     ERAKHWLERVGLIERADDPAGDLPYGAQRRLEIARAMCTEPVLLCLDEPAAGLNPRES
                     AELNALLRSIRDKDHVSILLIEHDMSVVMEISDHIVVLEYGAKISDGTPDFVRNDPRV
                     IQAYLGVDDEEVPAALGAAR"
     gene            4578..5297
                     /locus_tag="PK264_13815"
     CDS             4578..5297
                     /locus_tag="PK264_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD76985.1"
                     /translation="MPLLTVRGVETFYGKIQALKGVDIDVNRGEIVTLIGANGAGKST
                     LMMTIFGKPRARAGRIVYDGQDITTLETHDIARLRIAQSPEGRRIFPRMTVRENLQMG
                     AEIDGLAHFDEDLKRVTTMFPRLRERLDQRGGTLSGGEQQMLAIARALMSRPRLLLLD
                     EPSLGLAPIIVKQIFEVIRELNRSDRMTIFLVEQNAFHALRLAHRGYVMVNGVITMSG
                     TGQELLARPEVRAAYLEGGRH"
     gene            5338..5658
                     /locus_tag="PK264_13820"
     CDS             5338..5658
                     /locus_tag="PK264_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76986.1"
                     /translation="MPPALYEAGPNGVWTFLLATVLLGGLAAWVTGRSIAETWRPYWQ
                     IPVYMVMLAASVRFIHYAIFQAKLLSWRSFLVDLLVLLALAALSHVLARRRQMALQYG
                     WKTS"
     gene            complement(5659..>6516)
                     /locus_tag="PK264_13825"
     CDS             complement(5659..>6516)
                     /locus_tag="PK264_13825"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRD76987.1"
                     /translation="AARRLGIACSQGGEFAFVLLTAAVAGALVAGPTAELLAVVVTLS
                     MIVTPLALAIDDRFRRTDTAPSRAYDPLPDDASGVVIAGFGRVGQIVSRILRARRIPF
                     TALDSSAEHVDFVREFGSKIYYGDAARPDILRAAQLEKARVFVLAIDDVEQSLRTAEM
                     VRRQYPRLPIIARARNRQHAYRLLDLGIEQVHRETFEAALGISRDVLRGLGLRESELR
                     ATVERFKDYDERRLKEDYPLRSDMEKLRMRARNAAEELEQMFREDAASGAATPAALRP
                     AEPNSKSGA"
     assembly_gap    6517..6526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6527..7465)
                     /locus_tag="PK264_13830"
     CDS             complement(<6527..7465)
                     /locus_tag="PK264_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HRD76988.1"
                     /translation="MHDFLVQAAIFLLAAAVAAPLARRVGIGSVLGYLFAGVLIGPYG
                     LGRIYPVYSAESVLRYAELGVVLLLFVIGLELQPSRLKAMRAAVFGAGALQVVGTGLV
                     LTLGALIAGMSWGAAFYVGLALSLSSTAFTLQALGERKELATRHGRLAFAILLFQDLA
                     ALPILALVPLLAGAVSTAFSPMSVAKALAVVAAVLLLGRPILRHVYRLIARAGLHEAM
                     IATALLTVIVTALAMEAAGLSAALGAFVAGVLLADSEYRHEVEADIEPFKGLLLGLFF
                     TAIGMALNLGLLASRPVTILGLVVAVVAAKAAVLVAI"
     gene            7799..8914
                     /locus_tag="PK264_13835"
     CDS             7799..8914
                     /locus_tag="PK264_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD76989.1"
                     /translation="MKRFVLSGAALIAGLTLSGAANAQIKVGVAGPFTGPNAAFGAQL
                     KNGAEMAAADINAKGGMNGQKIELVFGDDASDPKQGVSVANKMVGDGVKFVIGHFNSG
                     VTMPASLVYAENGVLMITPSATNPQITERGLWNVFRTCGRDDQQGEVAGKYIASALKG
                     KKIAVIHDKTTYGKGLADETKKAMNALGVTEVLYEGVNVGEKDMSALVGKIKSSGADY
                     IYWGGLHTEGGLLVRQMRDGGLKTVLISGDGITTDEFATIGGPGVEGTLMTFPPDPQK
                     RPEAAAIVAKFLEKKFKPEAYTLYSYAALQIVADAANASKSNDAKKVAATMREGKPYA
                     TVIGAIAFNKKGDITRPDYVLYTWKKGADGKITYVQN"
     gene            complement(8998..9930)
                     /locus_tag="PK264_13840"
     CDS             complement(8998..9930)
                     /locus_tag="PK264_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRD76990.1"
                     /translation="MAKSRKVIITCAVTGSIHTPSMSPHLPVTAEEIADAAIGAAEAG
                     AAVVHLHARDPKTGKPDQSTEAFAPFLKVIKQRSNCVVNITTGGAMTMTIEERVRPAA
                     TFKPEVASLNMGTMNFGLYPMLERQKEFKHDWERPYLEGSRSGFFKNTFADIEYILTT
                     CAANGSRFEVECYDIGHLYTLRHFADRGIIKPPYFIQSVFGILGGIGPHYEDVNMMKR
                     TADRLFGDQYRWSVLGAGRNQLPIAAMAAAMGGNVRVGMEDSLWIGPGKLAKSNAEQV
                     TKVRQIIEGLGLEIASADEAREILALKGGDKVAF"
     gene            10107..10526
                     /locus_tag="PK264_13845"
     CDS             10107..10526
                     /locus_tag="PK264_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD76991.1"
                     /translation="MPRKRSISRRATLAGLSGLAAGMATKAHAAPRAELIMFDATWCG
                     YCKKFKREVAPTYSSTRAGGIFPLRIIDIERETPDFSLKEKVRGTPTFVIVADGIEIA
                     RFSGYGGPDHFYETMDRIISAWEQARQKWDRGGGDRA"
BASE COUNT         1880 a         3688 c         3443 g         1883 t
ORIGIN      
        1 ctgcctactg cctactgcct actgcctact gcctactgcc tactgcctac tgcctcttat
       61 tacacccgtt cgagcttctg cagcgatcgc agcggtcgcg gcatgggctt ctccggccnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgacg tagatgtcgc cgtggctgtc
      181 catcgcgagg ccatgaggcg cgatgaagcg tcccgtctcg gtgccgggcc cgtcctcgcc
      241 gccgaggcgc gcgacgagcc ggcccttggc atcgacgatc gagagtcgcg gcccgaggtt
      301 cttgtaggcg cggttgaccg gctgcgtcgg cccgagctcg ccgacgatga aggtcggcga
      361 cttggctccc ggcgggcacg agcagtagag gccgcatggc ctgtgcaagt tgtgccactc
      421 cgtctcgaag cggccgttgc cgtcgaacac ctgtacgcga tggttctcgc ggtcggcgac
      481 gtagacccag ccgtccttgt cggtgacgat gttgtggacg aggttgaatt gccccggccc
      541 cgagccggtc tcgccccagg tcttcaagag tttgccgtcc ggcgagaact tgtggacgca
      601 ggagttgccg tagccgtccg aaacgtagat ctcgcccttg ggtgagaggg ccgtgtgcgt
      661 gcagcggtgg aagggcttac ccgacatgaa cggagcaggc tttcccggca cgccgatctc
      721 gagcagcacc ttgccgtcgg tcgtgcactt cctgacgaaa tgtccgccgt cgtcggtgca
      781 gtagagatgg tcgttctcgt cgatgtgcag gccatgggcg cgcgggaact tgccctcgcc
      841 ccagctcttg atgaagttgc cgtcgcggtc gaacacgatc atcgggtgct cgccgcgatt
      901 gaacacgtag acccgatccc ggctgtcgca ggcgaccgag ccgacgtcca tgaactccca
      961 gccgtccggc agcttcgccc agttctcgac gacgcgataa gtgtgctctc ccgagccgag
     1021 gcggactgac atggcggtct cccgaatgct gcttggcaat ggcatctttc caaaagtcgg
     1081 caccaggcgc gaccacgaaa ttcgctcgtg gcgcatcttg atcgctggcc acaggctggg
     1141 gccggccggc cacggcccga acgtcaagct aactctgact gtgacagctg cgttgcactt
     1201 tttacatgcc gtctgctagc ttggcnnnnn nnnnnggtcg ggcggcgcgc ggaccggtca
     1261 acggaaggcg cctcgagggg gtacagtcat ccgatggaag tcttcctgca gcaggtgatc
     1321 aacgggatca cgctcggctc gatctacggc ctcatcgcca tcggctacac gatggtcttc
     1381 ggcatcatcg gcatggtgaa tttcgcccat ggcgacgtgt tcatgctggc gaccttcttc
     1441 gtgctgatcc tgctcctcct gatcatccag gcgataggcg ccgcggggca ggtgctcgct
     1501 ggcgcgacgc tcgtcattgc tctgctgctc gtgctggtcg gcgccatgtt gctcacctcg
     1561 ctctggaact gggtgatcga gcgcctcgcc tatcgcccgc tgcgcggctc cttccgcctc
     1621 gccccgctga tctcggcgat cggcatgtcg attttcctcg tcaacttcgt gcagctcgtc
     1681 cagggcccgc gcaacaaggc gctgccgccg atgatgcgcg agacgatcaa cctcaagtca
     1741 ctgatcggct acgacgtgac gctcagccac aagcagatct cgatcatcct gatcaccggc
     1801 atccttctcg ccatcttctg gtatctcgtg cagcacacct cgctcggccg cgctcagcgc
     1861 gcctgcgagc aggacggcaa gatggcggca ctgctcggcg tcaacgtcga ccgcacgatc
     1921 tcgctcactt tcgtgatcgc ggcagcactc gcggctgtcg cgggcgtcat gtacttcatc
     1981 cagtacggcg tggtgaaata cgatgacggc ttcgtgccgg gcgtgaaggc cttcaccgcc
     2041 gccgtgctcg gcggcatcgg ctcgttgccc ggagccgttc tcggcgggct tctcatcggc
     2101 ctcatcgagg tcttgtggtc gcagtatgtg tcgatcgact acaaggatgt cgcggccttc
     2161 tccatcctcg ccatcacgct gattttcctg ccgtccggac tgctcgggcg gcccgaggtg
     2221 gaaaaggtct gagcgccatg gcggatacga ccacggagac ctcgtcagtc gacttcggca
     2281 aagccttgcg cgaagcgctg atcacggccg cactcacact cgggctcgcg gtgccgatcg
     2341 tcctgctgcg caccgagcag gatatgaaca acacgctgat cctgcaatgg cggtggggtc
     2401 tcgttgcggc actcgttggc atcgcattcg cggctcgcct cggctgggag ctcgtcgggc
     2461 cccacttgag gggtggcggc tcaagcggca aggcgcagcg ccacgccgca atgccgcgat
     2521 ccgcccagac ggctctggcg attttcggcg ttgccttact cgtcgcctat ccaatcttcg
     2581 tactgtggcg ccttggtccc tccggctcga tcaagtggat cgacaacttc ggcatccaga
     2641 tcctgatcta cgtgatgctc ggctgggggc tcaacatcgt cgtcggcctc gccgnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcggcaac ctgctcggtc cgaacttctg
     2821 gccgctctgg gccttctgga tatgcttgcc cgtcgccggc atcctcgccg cgttctgggg
     2881 cgtgattctc ggatttccgg tgctccgcct gcgcggcgac taccttgcca tcgtcacgct
     2941 cgcgttcggc gaaatcatcc ggctgatcct catcaactgg gtgcccgtca ccggcggcta
     3001 ccagggcatc gggcacacgc agccgatccc gcgaccgagc ttcttcggca tcccgttcaa
     3061 cgcgtcggat gagggctttt cggcccgttt cggtctcgag ttctcgccga tctaccgcac
     3121 catcttcctc ttctacgtca tcgcagcgct ggcgctgctg acaaactggg tgacgctgcg
     3181 gctccgccgg ctccctgtcg ggcgcgcctg ggaggcgctc cgcgaggacg agatcgcctg
     3241 ccgctcgctc ggcatcaaca cgaccaatac gaagctcacg gccttcgcga tcggtgccgg
     3301 ctttggcggc atcgccggct cgttcttcgc cgcccgccag gcgttcatct cgcccgaaag
     3361 cttcaccttc atcgaaagcg cgctcatcct gtggatcgtc gtgcttggcg gcatgggctc
     3421 gcagatcggc gtcgcgatcg ccgccgtgat catgatcggc ggcacggagc tcttgcgcga
     3481 actcgactac ctcaaagtgc tgttcggcaa ggacttcgat ccgacccact accgcatgct
     3541 gctgttcggg ctcgcaggcg tcctgctcgt gcgctggaag ccgcgcgggc ttgtcgcgac
     3601 ccgctcgccg acgatcgttc tcaaggagcg ccgcagcata tcggccgacc tcgtcaagga
     3661 agggcacggt tgatggcgtc ggcacccctc ctcactgtcg agcacatgac gatgcgcttc
     3721 ggcggcctcg tcgcgatcaa tgatctctcg ttcacggcca agcgcggcga gatcaccgcc
     3781 atcatcggcc cgaacggcgc cggcaagacc accgtcttca actgcatcac cggcttctac
     3841 aagccgaccg tcggccggat ggcactggca atcagcgcgg ctgaggccgg cgcgatggag
     3901 gcgctgacca agaccggtcg tcgcttcgat gccgcgagcg gcatcttcct gctcgagcgc
     3961 atgccggatt tcgagatcgc ccagcgtgcc cacatcgcgc gcacattcca gaacatccgc
     4021 ctgttctcgg gcctgacggt cctcgagaac ctgatcgtcg cgcagcacaa ccgcctgatg
     4081 agcgcttcgg gctggacgat caagggcctc ctcggctcgc gacgctacaa gacagccgaa
     4141 cttgcggccg tcgagcgcgc caagcattgg ctcgaacgcg tcgggcttat cgagcgcgcc
     4201 gacgatccgg ccggcgacct cccctatggc gcgcagcgcc ggctcgagat cgcccgcgcc
     4261 atgtgcaccg aacccgtgct cctctgcctc gatgagccgg cagccggact caacccgcgc
     4321 gaatccgccg agctcaacgc gctgctgcgc tcgatccgcg acaaggacca cgtctccatc
     4381 ctgctcatcg agcacgacat gagcgtcgtc atggagatct cggaccacat cgtcgtgctc
     4441 gagtacggcg cgaagatctc tgacggcact cccgacttcg tgcgcaacga cccgcgcgtc
     4501 atccaggcct atctcggcgt cgacgacgag gaagtgcccg ccgccttggg agccgcacga
     4561 tgagctcgga gagagcaatg ccactcctca ccgtgcgcgg cgtcgagacc ttctacggca
     4621 agatccaggc cttgaagggc gtcgacatcg acgtcaaccg cggcgagatc gtcacgctga
     4681 ttggcgccaa cggcgcgggt aagtccacac tcatgatgac gatcttcggc aagcccaggg
     4741 cgcgcgccgg ccggatcgtc tacgacgggc aggacatcac gacgctcgag acgcacgaca
     4801 tcgcgcgcct caggatcgcg caatcgcccg aaggccgccg catcttcccg cgcatgacgg
     4861 tgcgcgagaa tctgcagatg ggagccgaga tcgacggcct tgcccacttt gacgaggacc
     4921 tgaagcgcgt cacaacgatg tttccgagac tgcgcgagcg cctcgaccag cgcggcggta
     4981 cgctctccgg cggcgagcag cagatgctcg ccatcgcccg cgcgctgatg agccggccgc
     5041 ggctcctcct tctcgacgag ccgtcgctcg gtctcgcgcc gatcatcgtg aagcagatct
     5101 tcgaggtgat ccgcgaactc aatcgctccg atcgcatgac gatcttcctc gtcgagcaga
     5161 atgccttcca cgcgctccgc ctcgcgcatc gcggctacgt gatggtcaac ggcgtgatca
     5221 ccatgtctgg gacggggcag gaactcctcg cccgcccgga ggttcgagcc gcctatctcg
     5281 aaggcggacg gcattaggag acagacgggc atgctcgcgt cattcacgcc ttggccgatg
     5341 ccgccggcac tctacgaggc tggcccgaac ggagtgtgga ccttcctcct cgcgaccgtg
     5401 ctgctcggcg ggcttgccgc ctgggtgacc ggccgctcga tcgccgagac gtggcggccc
     5461 tactggcaga tcccggtcta catggtgatg ctcgcggcga gcgtgcgctt catccactat
     5521 gcgatcttcc aggctaagct gctctcgtgg cgaagcttcc tcgtcgatct gctcgtcctg
     5581 ctggcgctgg ccgcgctctc gcatgtgctc gcacggcggc ggcagatggc cctgcagtac
     5641 ggctggaaaa cgagttgatc acgcgcccga cttgctgttc ggctcggccg gcctaagcgc
     5701 tgcaggcgtc gcggccccgc tcgcggcatc ctcgcgaaac atctgctcga gttcctcggc
     5761 ggcattgcga gcgcgcatgc gcagcttctc catgtcagag cgcagcggat agtcctcctt
     5821 gaggcggcgc tcgtcatagt ccttgaagcg ctcgactgtt gcccggagct cgctctccct
     5881 gaggccgaga ccgcgcagca cgtcgcgtga aatgccgagt gccgcctcga atgtctcgcg
     5941 gtgaacctgc tcgataccga ggtcgagcag ccgataggcg tgctggcgat tgcgggcacg
     6001 cgcgatgatc ggaagccttg ggtactggcg acgcaccatc tcggccgtcc tcagcgactg
     6061 ctcgacgtcg tcgatggcga gcacgaagac ccgggccttc tccagttggg ccgcccgcag
     6121 gatgtcgggg cgcgccgcat cgccgtagta gatcttcgag ccgaactcgc gcacgaaatc
     6181 gacatgctcg gcgctgctgt cgagggcggt gaacgggatc cggcgggctc tcaggatgcg
     6241 cgagacgatc tgcccgacgc gcccgaagcc agcgatgacg acacccgagg catcgtcggg
     6301 cagcggatca taggcccgac tcggtgctgt gtcggtccgc cggaagcggt cgtcgatcgc
     6361 cagtgccagt ggcgtcacga tcatcgacag cgtgacgacg acagcgagga gctcggccgt
     6421 cggcccggca accagcgcac cggccaccgc cgccgtcagc agcacgaacg cgaactcgcc
     6481 gccctgcgag catgcgatgc cgagccgccg cgctgcnnnn nnnnnngatc gcgacgagga
     6541 ccgcggcctt tgcagcaacg acggccacga cgaggccgag aatggtcacc ggcctcgagg
     6601 ccagcagacc gagattgagc gccatgccga tcgccgtgaa gaagagcccg agcaggaggc
     6661 ccttgaacgg ctcgatatcg gcctcgacct cgtggcggta ctcgctgtcg gcgagcagca
     6721 cgccggcaac gaacgcgccg agcgcggccg atagccctgc cgcctccatc gccaaggccg
     6781 tgacaatcac cgtcagcagt gcggtcgcaa tcatggcctc gtgcaggccg gcacgtgcaa
     6841 tcagccgata gacatgccgc aggatcggcc gaccaaggag gagcacggcg gcaacgaccg
     6901 ccaatgcctt cgcaacggac atcggcgaga aggcggtcga taccgcgccc gccagcagcg
     6961 gcacgagtgc caggatcggc agggcggcga ggtcctggaa gagcaggatt gcgaacgcga
     7021 gccggccgtg ccgcgtcgca agctccttgc gctcgccgag ggcctgcaac gtgaaggccg
     7081 tcgacgacaa cgacaacgcg agccccacgt agaacgccgc accccacgac atgccggcaa
     7141 tcaacgcgcc gagcgtcagg accagtccgg tgccgacgac ctgcagcgct cccgcaccga
     7201 atacggcggc gcgcatcgcc ttcaagcgcg acggctgcaa ctcgagcccg atgacgaaga
     7261 gcagcaggac gacgccgagt tcggcataac gcagcacgct ttcggccgaa tacacgggat
     7321 agatccgccc gaggccataa ggcccgatca gcacgcccgc gaacaggtag ccgagaacgg
     7381 acccgatacc gacacgccgc gcgagcgggg ccgcgacagc tgccgccaac agaaaaatgg
     7441 ccgcctggac cagaaagtcg tgcatgattc attccctttc ccgcgctcga cggctccatc
     7501 ccgcccgcgg cgcgcgccca caacgatgac ccaatccctg gcccttgtcc ggctgccggc
     7561 caaccgacat gaggacaccc gagaactcgg gcgaattcgg atgactcttg ccacaatctt
     7621 ggttcataag cgcgctaggc gggcttgcca cgaagggtcg ggcgctaacg gcggccgaaa
     7681 cggggttgct cgcgatcgtg tctgccgccc ggcgacgggt ggtcggccgc agttggacta
     7741 cgcaagttgg actacgaatg atctttcagt tcgcatgaac ctaatgggag tgaggtccat
     7801 gaaacgtttc gtgctgtcgg gtgccgcgct gatcgcgggc ttgacactgt cgggcgccgc
     7861 aaatgcgcag atcaaggtcg gcgtcgccgg tccgttcacc ggcccgaacg ccgctttcgg
     7921 cgcgcagctg aagaacggcg ccgagatggc cgcggccgac atcaatgcca agggtggcat
     7981 gaacggccag aagatcgagc tggttttcgg tgacgatgcg tcggatccga agcagggcgt
     8041 gtcggtcgcc aacaagatgg tcggcgacgg cgtgaagttc gtgatcggtc acttcaactc
     8101 cggcgtcacg atgccggcgt cgctcgtcta tgccgagaac ggcgtcctga tgatcacgcc
     8161 atctgcgacg aaccctcaga tcaccgagcg cggcctctgg aacgtgttcc gcacctgcgg
     8221 ccgtgacgat cagcagggcg aggttgccgg caagtacatc gcctcggcgc tgaagggcaa
     8281 gaagatcgcc gtcatccacg acaagacgac ctacggcaag ggcctcgccg acgagaccaa
     8341 gaaggcgatg aatgccctcg gcgtaaccga ggtgctctat gagggcgtca acgtcggtga
     8401 aaaggacatg tccgcgctcg tcggcaagat caagtcttcc ggcgccgact acatctactg
     8461 gggcggcctc cacaccgagg gcggactgct cgttcgccag atgcgcgacg gcggtctcaa
     8521 gacagtgctg atatcgggcg acggcatcac gacggacgag ttcgctacca tcggcggccc
     8581 gggcgtcgag ggcacgctca tgacgttccc gcctgacccg cagaagcgcc ccgaggccgc
     8641 ggccatcgtc gcgaagttcc tcgagaagaa gttcaagccc gaagcctata cgctgtacag
     8701 ctacgcagcc ctccagatcg tcgccgacgc ggccaacgcc tcgaagtcga acgatgccaa
     8761 gaaggttgca gccacgatgc gcgagggcaa gccctatgcg acggtgatcg gcgccatcgc
     8821 cttcaacaag aagggtgaca tcacccgtcc ggactacgtg ctctacacct ggaagaaggg
     8881 cgcggacggc aagatcacct acgtccagaa ctgaactgat cgacccgtga aaaggccgat
     8941 gggcgagggg ccaccgcagc tgcggcggcc ccttctgctt tcacacgacg tgcacgctca
     9001 aaacgcgacc ttgtcgccgc ccttcaaggc caggatctcg cgggcctcat ccgcgctcgc
     9061 gatctcgagg ccgaggcctt cgatgatctg gcgcaccttc gtcacctgct cggcattcga
     9121 cttcgcgagc ttgccaggtc cgatccagag gctgtcctcc attccgacgc ggacgttgcc
     9181 gcccatggcc gccgccattg ccgcgatggg cagctggttg cgcccggcgc cgagcacgct
     9241 ccagcggtac tggtcgccga acaggcggtc agccgtacgc ttcatcatgt tgacgtcctc
     9301 gtagtgcggg ccgatgccgc cgagtatgcc gaacaccgac tggatgaagt acggcggctt
     9361 gatgatgccg cgatccgcga agtggcgaag cgtatagaga tgcccgatgt cgtagcactc
     9421 cacctcgaag cgcgagccgt tggcggcgca ggtcgtcagg atgtactcga tatcggcgaa
     9481 ggtgttcttg aagaagcctg accgcgagcc ttcgagatag ggccgctccc aatcgtgctt
     9541 gaattccttc tggcgctcga gcatcgggta gaggccgaaa ttcatggtcc ccatgttgag
     9601 cgaggcgacc tccggcttga aggtcgcagc ggggcgcacg cgctcctcga ttgtcatggt
     9661 catggcgccg cccgtcgtga tgttcacgac gcagttggag cgctgcttga ttaccttgag
     9721 gaacggcgcg aaggcctccg tcgactggtc gggcttcccc gtcttgggat cgcgggcatg
     9781 caagtgcacg acggcggcac cggcctcggc ggctccgatt gcggcgtccg cgatctcctc
     9841 agccgtcacc ggcagatgcg gcgacatcga cggcgtgtgg atcgagcccg tgaccgcaca
     9901 ggtaatgatg actttgcgtg attttgccat gatcgtcctc ttcctgaagt cttgaagccg
     9961 ccggccatcc ggaccgcatg aaaagcctat cgcgaagcac gggacggcga aacaacgttg
    10021 ctgctatagt gccccccgtg gccggatgcc tcgaattggt cacgagccgc atccaaagtc
    10081 gctgcgcatt caactggagc cgacgcatgc cgcgcaaacg ttcgatcagc cgcagggcca
    10141 cacttgccgg cctatcgggc ctcgccgcag gcatggccac caaggcccat gcggcgccgc
    10201 gcgcagagct catcatgttc gatgcaacct ggtgcggcta ttgcaagaaa ttcaagcgcg
    10261 aggttgcgcc cacctactcg agcacgcgcg ccggcggaat atttccgctg cgcatcatcg
    10321 acatcgaacg cgagacgccg gacttctcct tgaaggagaa ggtgcgcggc acgccaacct
    10381 tcgtcatcgt cgccgacggc atcgagatcg cgcgcttctc aggctatggt ggcccagacc
    10441 acttctacga aaccatggac aggatcattt cggcctggga gcaggcgcga cagaagtggg
    10501 accgaggcgg aggcgatcgc gcctgaggca ggccggctgc tgtgtggatc aagagcaacc
    10561 attgaacgtc gccgcgggcg ttgccgcgac agagggattg tgtgatgggc gagcatgcaa
    10621 ggatgggccg gccgggggca tcgcggctga cagcgtacgc actggcagtc atgctcggcg
    10681 cgggcgccgc aggagtggcc atggcgcagg atacgccgag gccgagcggt gacccgtcgt
    10741 cgcctcctgc cccggcctcg agtccgacca atccgtcatc tccgaccacg agcaagccgc
    10801 cgcgggacgg cgatgcaccc gtgtccggcg cgcacactcc atcgccgaat gccgcgcctc
    10861 caccggtcga tcggccggct gcagccgctc cggcatcgac aggcacagcc tcgggcggcc
    10921 cggcggcagc tgctgctccc ccggcgccga gatccgacac tccggcgaca gcgccatcga
    10981 ctccccccgc gccggctccg tcatcgccgc cggtcgttgc cgcaccagcg cccttgccac
    11041 caccagcg
//
LOCUS       DAORNH010000133        11039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4446_length_11039_cov_2.401038, whole genome shotgun sequence.
ACCESSION   DAORNH010000133 DAORNH010000000
VERSION     DAORNH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4446_length_11039_cov_2.401038"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..641)
                     /locus_tag="PK264_13850"
     CDS             complement(294..641)
                     /locus_tag="PK264_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76992.1"
                     /translation="MILEQEGTLKHVIRRIVALSIIAGTGMLVPASAHDWYPHDCCND
                     MDCAPVESAEWLAPTAGGLPQLVVTSKHGRARVRKGASIRASKDNRMHVCMKRYDPLG
                     EMEVVCLFLPTDA"
     gene            1072..2559
                     /locus_tag="PK264_13855"
     CDS             1072..2559
                     /locus_tag="PK264_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRD76993.1"
                     /translation="MLLALVERRGQVVSKDTLMAEGWPDRVVEENNLQGQISALRKVL
                     AADSDGERYLQTVPGRGYRFVAPVEHERSAAGTIFEVSARRDALAWCAPLHSKPAIAV
                     IPFINLSSDPQQEYFSDGITDDIVTELSRSSELHVIASNSSFKLKGRDVDVRQVGSRL
                     GVSYLLEGSVRKVGQHVRITAKLVDAINGAHCWAERYDRELMDVFAVQDEVARTIVSI
                     LTAHVGKLEAARVLARPPQTWQAYDYYLQGRESFASFLTTYRAADLYEARRHLQSALA
                     IDPNYARAHAVLSNIHLCGYIHRLDDDYLTDAALERAHEIARKAVQLDPNLTVAREHL
                     GLVLSHMGRHAEALAEFEKVTALNPNFSNWRYGLALVYGGEAEKAIKVLRAITHLDPF
                     YPAAAAGFFGVAYYTLKRYAEAQPWLLECTSRAPNFRAGHSWRAANHAQLGQLDEARR
                     DVSEVLRLDPTFTIDHTVRRLAQFRRPEDADHFFDGLRKAGLPVQ"
     gene            complement(2801..4093)
                     /locus_tag="PK264_13860"
     CDS             complement(2801..4093)
                     /locus_tag="PK264_13860"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HRD76994.1"
                     /translation="MSDESPRFDTLSLHAGQRPDPTTGARAVPIYQTTSYVFRDTEHA
                     AGLFNLERAGHIYSRISNPTVAVFEERMAALEGGVGAVATASGQAALHLAIVTLMGAG
                     GHIVASARVYGGSHNMFTHTLPRFGITTTQVDPRDPAAFARAIRPETRLVFTETLGNP
                     GLEVSHIPVIANLAHEAGVPLMVDATFSTPYLCRPITFGADIVMHSATKFIGGHGVAI
                     GGVIVDGGRFDWRRSGRFPTMTEPYAGYHGIVFAEEYGPSAFIMRARAEGLRDFGAAM
                     SPTTAFHLIQGLETLPVRMARHVANAETIARALEKHSAVAWVKYPGLESHPDHELAKR
                     LLPKGAGAIMSFGVKGGRAAGAKLIERLRVFSHLANVGDAKSLVIHPASTTHQQMSAE
                     ELTRAGVGEELVRLSIGLEDVADLVEDLDQALKASQKG"
     gene            complement(4136..4405)
                     /locus_tag="PK264_13865"
     CDS             complement(4136..4405)
                     /locus_tag="PK264_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD76995.1"
                     /translation="MTELQTVLLFGFANPIAVAMAFWLGRSADQPQKSVIGGFLGGLI
                     AVLLAAAAASVGWIKLLPRSYSGVFVCAFAVGMLAAWLGYRTRRR"
     gene            complement(4415..6016)
                     /locus_tag="PK264_13870"
     CDS             complement(4415..6016)
                     /locus_tag="PK264_13870"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD76996.1"
                     /translation="MYGRRPTITSVHGMVAAAHPLAAQAGARLLASGGNAFDAAVATA
                     AALNVVEPYMSSLAGQGLATVYSAKDKRVRCLDFVPRMPRKFPVDKLSSREQLQRGAI
                     GTGVPGNFGGWAELVKAYGTKPLSEILAPAVALARDGYPLIEFNIYETNEARAQLAAH
                     PDLYPEWVKTYTFGRGSVELGELLKQDDLAKTLDALGKEGPGLLYGGTLGKAIVARLT
                     ALGGVMEMADLEAFKPIWREPLTVAYRGLDISCPPPPCEGFQFSLTMRILEGFDLARL
                     PRNGVEHLDIVWRAIRLAAGVRLAHNGPSPERLAEMLSDANVAKLRARVEDGKPIVGP
                     TEQWTAPASTGAADQHTTSFSIADKAGNLVCITQSIGSPFGSGVIVPGTGLCLNNFLY
                     WSDVNPKSPNRATPGGDLHMCVTPTISMRGKDAVLALGTPGSYGILQTQVQALVQHLD
                     YGLPLQDAIEAPRARLWDGAIVEPEARFEQPVLDQLRRRGHAVTPGEPWTMRVGGMQA
                     VSRDPKTGILVGACDPRRDGYVATP"
     gene            complement(6139..6792)
                     /locus_tag="PK264_13875"
     CDS             complement(6139..6792)
                     /locus_tag="PK264_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRD76997.1"
                     /translation="MPVNIDLAVLGAFVLLWAVIVPTPGANSLMVTHVALTRSAADVA
                     MAIAGNMVGNVLLAGAALLGMAALLATFPWLRLAMHVLGGAYLIYFGVRLLLRSRNAP
                     EGEPLPDTAPAPGVPLWRTFSLGFGTALSNAQAIVFLTSIFAATRVLDASLATGIACI
                     AAIVAMNASYLSLLGWLFQRAAVRRGYARMRSGAEAVVGGLFVAFGLRLIWRELAAR"
     gene            complement(6825..>7830)
                     /gene="trpD"
                     /locus_tag="PK264_13880"
     CDS             complement(6825..>7830)
                     /gene="trpD"
                     /locus_tag="PK264_13880"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRD76998.1"
                     /translation="LRDDFFDVQGADGLPARAAFQLMTEGVATQAQMGAFLMALRVRG
                     ETVSELTGAAELMRSKMLRVHAPPGAVDIVGTGGDAHGTYNVSTCASLVAAGAGLKIA
                     KHGNRSVSSISGASDVLAALGVKLDVAPPVVERAIVEAGVGFMWAPMHHPAMKHWAPV
                     RAELGIRTIFNLLGPISNPAGVKRQIVGVFSWQWVEPIALVLRNLGAEHVWVVHGHDG
                     LDELTTTGSTDVAEVKGGEIRVFEVTPADAGIAPAKLSDLKGGDAKTNAAAIRDVLAG
                     KKGPFRDIVLLNSAAALIVGGAAANLTDGAALAAHSIESGAAMRALERLVAVSNATSG
                     "
     assembly_gap    7831..8025
                     /estimated_length=195
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8035..9224
                     /locus_tag="PK264_13885"
                     /pseudo
     CDS             8035..9224
                     /locus_tag="PK264_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
     gene            9237..10187
                     /locus_tag="PK264_13890"
     CDS             9237..10187
                     /locus_tag="PK264_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6384 family protein"
                     /protein_id="HRD76999.1"
                     /translation="MTTEAAGEPAPGAAKSPGTLDDVMIAMDVVDTVRHREDLVRREL
                     NEAGREADLIARLREIYRQQGIEVPDHVLAEGVKALRESRFTYTPPPKGLKRTLLTLW
                     TRRRTVGIGAGAAAAAVVGYQLAIALPAREEARRQETEITVTLPKALKDAYADVLAVS
                     TDPGAKGRADALLADGERALRERSAAAARESNARLKALRDELAQEYTLTIVARKGEAM
                     GVWRTPPKSQTGRNYYLIVEAITPDGRALPLPVRNEETGKTETVTKLGVRVPEATFSA
                     AAADRRDDGIVQNNRFAIKRRGVLAVEYLMPFEGGMITAW"
     gene            10200..>10627
                     /locus_tag="PK264_13895"
     CDS             10200..>10627
                     /locus_tag="PK264_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77000.1"
                     /translation="MPSGRQTLRTLETSISGINREQETLEEAVRAASAEVARLRRERI
                     AALRELARVKLDEMAAGRLTRDLDLAERRVTEMMAARERALTESAEEQKRRVAAVEAA
                     EATRDEAAKRVEALVKEVEDTRLAAETAVEREPAWLQANAA"
     assembly_gap    10628..10703
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10704..>11039
                     /locus_tag="PK264_13900"
     CDS             <10704..>11039
                     /locus_tag="PK264_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77001.1"
                     /translation="KPYDEDPLFAYLWGRGYLTPRYHAGNVARMMDGLVAEFIGYSNA
                     RPNYVMLSEIPVRLGAHATSRRAAADELKAARGAIERQRMVALGIEPKERALAAARAE
                     LKTADNALEA"
BASE COUNT         1904 a         3495 c         3678 g         1691 t
ORIGIN      
        1 gtgcgccggg ggagggcggg cagcgctgcg ggtggagacg gtcatgccct gaacgggcgt
       61 cgtgcgtccc agccgccaca tgctccgacg ttgtacttgg gttcgtcgcg agtaccgagt
      121 gtgccgcagc ggtgatccgt cgtgatgggc cactttcgac cgacgtgcag cgagcttcct
      181 tcggctttca tctcggcagc acttggatgg gcaggcagac cgaagaacta cctggatcga
      241 ctagacgatc agcgcgccgc gtcgggggcg agcgcagccg cgcattgtcg gactcacgca
      301 tccgttggca agaagaggca aaccacttcc atctctccga gcggatcata gcgcttcatg
      361 cagacatgca tgcggttgtc tttcgatgct cggatagatg ctcctttgcg aactctagct
      421 ctgccgtgct tcgacgtcac aacgagttgc ggcaggcctc ctgccgtcgg cgcgagccac
      481 tccgcactct cgaccggcgc gcaatccatg tcgttacaac agtcatgcgg ataccagtca
      541 tgggcgcttg ccggcacaag cattcctgtg ccagcgatga tcgacaaggc cacaatccga
      601 cgaatgacgt gcttcaacgt tccctcctgc tcgaggatca ctcggagttc cggactggcg
      661 cataaggctg ccaggttccg tggacgcctg aattgccctg cgtcccggaa tcatccgtgc
      721 tacgaaggct cttgacggac tgaacggtgg ccgagcgggc tcggtacgtg agcttgttgt
      781 cgttcgagaa ccgcgaggcc gcttttcttt tgccgtcgat ggggacactg tgaggccgat
      841 gggcctgtaa ggtcattgcc tcggatctga attgtcctga attctcctgc agtcgggtgc
      901 caagggtgac aagcgacgcc aatcacacgt ctcgagccac gacgggtctg cgatcccttc
      961 cgtgcgacgc tggggagtac cggtttggtc tcttccgcct gttgatcgag cggcgggagc
     1021 tgctgttgcg cgacgtgccg gttcacccgc acgtgctcgc gcgcgctcga tgtgctcctg
     1081 gcgctcgtcg agcggagagg tcaagtggtg agcaaggata cgctcatggc cgagggctgg
     1141 cccgacagag tcgtagagga gaacaacctt caggggcaga tttcggctct acgcaaggtg
     1201 ttggccgccg acagcgatgg cgagcgctat ctgcagacag tgcccggccg cggctaccgg
     1261 ttcgtcgccc cggttgagca tgagaggtcc gccgccggca caatcttcga ggtgagtgcg
     1321 cggcgcgatg cgcttgcctg gtgtgcgcct ttgcacagca agccagcgat cgcggtgatc
     1381 cccttcatca atctgagcag cgatccgcag caggagtatt tcagcgacgg catcaccgac
     1441 gacatcgtca ccgagctgtc gcggtcctca gagttgcacg tcatcgccag caactcgagt
     1501 ttcaaactga agggccgcga tgtcgacgtc cggcaagtcg gaagtcgact aggcgtcagc
     1561 tatttgctgg aaggcagtgt ccgaaaggtt ggacagcacg ttcgcatcac agccaagctc
     1621 gtcgatgcca tcaacggcgc tcattgttgg gcggaacgct acgatcgtga gctgatggac
     1681 gtattcgcgg tgcaggacga agtcgcgcga accatagttt cgatcctgac tgcgcatgtg
     1741 gggaaattag aagccgcgcg cgtcttggca cggccgcccc agacctggca ggcatatgat
     1801 tactacttgc aggggagaga gtcgtttgcc tcgttcttga cgacgtatcg agcggccgac
     1861 ttgtacgagg cccgacggca cctgcagagc gccttggcta tcgacccgaa ttatgcccgc
     1921 gcccatgccg tgctttcgaa cattcatttg tgcggctaca tccatcggtt ggacgacgac
     1981 tatctgacgg atgccgctct ggagcgagca cacgagatcg ccaggaaggc cgtgcagctc
     2041 gatcccaacc tgacagttgc gcgcgaacac ctggggcttg tcttgagtca catgggccgg
     2101 cacgccgagg cgcttgccga gttcgagaag gttacagccc tgaacccaaa tttttccaac
     2161 tggcgttatg gtctggcgct cgtttacgga ggcgaagccg agaaggccat caaggtcttg
     2221 cgcgcgatta cgcacctcga ccccttctac ccggcagcgg cggccggttt cttcggtgtt
     2281 gcctactaca cactcaagcg gtacgccgag gcgcagccgt ggctgctgga atgcacctcc
     2341 cgggcaccga actttcgagc tgggcatagc tggcgggcag ccaaccacgc gcaattgggc
     2401 caactcgacg aggcgcgcag agacgtgtcc gaagtcctcc gtctcgaccc gacgttcacg
     2461 atcgaccaca ccgtacggcg acttgcgcag ttccggcgcc cggaagacgc cgatcatttt
     2521 ttcgacggtt tgcgcaaggc gggattgccg gtgcagtaga tatgatcgct gcgaatttga
     2581 tcaggtggag cagcaggtct tggctgcgtc agcatctgca acaagcggac acgatagaga
     2641 ctttcatcgt acgacagctt cctgttcagc aacggggcat tcacgactca gcgtaggaag
     2701 tcgcgccagc ggacttcatt acatgcccga ggatggctgc tttgagcgtt gctgctcagg
     2761 cgcaagcagt ttctcttgcg cccatccgcc accagccccc tcaccccttc tgcgacgcct
     2821 tcagcgcctg atctaggtcc tcaacgagat cggcgacatc ttcgaggccg atcgagaggc
     2881 ggacgagttc ctcgccgacg cccgcgcggg tgagctcctc cgcgctcatc tgctgatgcg
     2941 tcgtcgaggc cggatggatg acgagcgact tcgcatcgcc gacgttggcg agatgggaga
     3001 agacgcggag ccgctcgatc agcttggcgc cggccgcccg cccgcccttc acgccgaagc
     3061 tcatgatcgc gccggccccc ttcggcaaaa ggcgcttggc gagctcgtga tcggggtggc
     3121 tttcgaggcc cggatacttc acccaggcga cggctgaatg cttctcgagc gcgcgcgcga
     3181 tcgtctcggc gttggccaca tgccgcgcca tgcgcacggg cagcgtctcg agcccttgga
     3241 tcaggtggaa cgccgtcgtc gggctcatcg ccgcgccgaa gtcgcgcagg ccctccgcgc
     3301 gcgcccgcat gatgaaggct gaggggccgt actcctccgc aaagacgatg ccgtgatagc
     3361 cggcgtaggg ctccgtcatg gtcgggaagc ggcccgagcg gcgccagtcg aagcggccgc
     3421 cgtcgacgat cacgccgcca atcgcgacgc catgcccgcc gatgaacttc gtcgcggaat
     3481 gcatcacgat gtcggcgccg aacgtgatcg gccggcagag gtagggcgtc gagaaggtgg
     3541 catcgaccat cagcggtacg cccgcctcgt gagccagatt ggcgatcacc gggatgtgcg
     3601 agacttcgag gccaggattg ccgagcgtct cggtgaagac gaggcgcgtc tcggggcgga
     3661 tggcgcgcgc gaaggctgcc ggatcgcgcg ggtccacctg cgtcgtggtg atgccgaagc
     3721 gcggcagcgt gtgcgtgaac atgttgtgcg agccgccgta gacgcgcgcg gaggcgacga
     3781 tgtgcccgcc ggcgcccatc agcgtcacga tggcgagatg gagggccgcc tgaccgctcg
     3841 ccgtcgcgac tgcgccgacg ccaccctcca aggccgccat gcgctcctcg aacacggcga
     3901 ctgtcgggtt cgagatgcgc gagtagatat gccccgctcg ctccaggttg aagagacctg
     3961 cggcgtgctc cgtatcgcgg aagacatacg aggtcgtctg gtagatcggc accgcgcgcg
     4021 cgccggtcgt gggatcaggg cgctggccag cgtgcagcga cagcgtgtcg aagcgggggc
     4081 tctcgtcgga catggtcgct cctcgggacg gcgtcgatgc ccgggagagt acccgctatc
     4141 gtcgacgcgt gcgatacccg agccaggcag cgagcatgcc gacggcgaag gcgcacacga
     4201 acacacccga ataggagcgc ggcagcagct tgatccagcc aacgctcgct gctgccgccg
     4261 cgagcagcac ggcgattaaa ccgccgagga agccgccgat cacggatttc tgcggttgat
     4321 cggccgagcg gcccagccag aaggccatgg cgacggcgat cggattggcg aacccgaaga
     4381 ggagaacggt ctgcagctcg gtcacgggtg tgcctcaggg tgtcgcgacg tagccgtcgc
     4441 ggcggggatc gcacgcgccg acgaggatac cggtcttcgg atcacgcgag acggcctgca
     4501 ttccgccgac gcgcatcgtc cagggctccc ctggcgtgac ggcatggccg cggcggcgca
     4561 gctgatcgag gacaggctgc tcaaatcgcg cctcgggctc gacgatggcg ccgtcccaga
     4621 gacgggctcg cggcgcctcg atcgcgtcct ggagggggag gccatagtcg agatgctgaa
     4681 caagagcctg cacctgcgtc tgcaggatgc cgtagctccc cggcgtgccg agggcgagga
     4741 cggcatcctt gccacgcatg gagatggtcg gcgtcacgca catgtggagg tcgccgcccg
     4801 gcgtcgcgcg gttcgggctc ttcgggttga cgtcggacca gtaaaggaag ttgttgaggc
     4861 agaggcccgt gcccggaacg atcacgccag agccgaaggg gctgccgatg ctctgcgtga
     4921 tgcagacgag gttgccggcc ttgtccgcga tcgagaacga ggttgtgtgc tggtcggcgg
     4981 cgcctgtcga tgcgggcgcg gtccactgct cggtcgggcc gacgatcggt ttcccgtcct
     5041 ccacgcgggc gcggagcttc gcgacgttgg cgtccgagag catttcggcg agccgctcgg
     5101 gcgatgggcc gttatgggcc aggcggacgc cggccgcgag gcggatcgcc cgccaaacga
     5161 tatcgaggtg ctcgacgccg ttgcgcggca gccgcgcgag atcgaagccc tcgaggatgc
     5221 gcatggtcag cgagaactgg aagccttcgc agggtggcgg cggacaggag atgtcgaggc
     5281 cgcgataggc gacggtcaga ggctcgcgcc agatcggctt gaatgcctcg aggtcggcca
     5341 tctccatgac gccgcctagc gccgtgagac gggcgacgat cgccttgccg agggtgccac
     5401 cgtagaggag gccggggccc tccttgccga gggcatcgag cgtcttcgca aggtcgtcct
     5461 gcttgagcaa ttcgccgagc tccaccgagc cgcggccgaa ggtgtaggtc ttcacccact
     5521 ccggatagag gtcggggtgg gccgcaagct gcgcgcgagc ctcgttcgtc tcgtagatgt
     5581 tgaactcaat cagcgggtag ccgtcacggg cgagcgcgac ggccggcgcc aggatctcgg
     5641 aaagcggctt cgtgccgtag gctttgacga gctccgccca gccgccgaag ttgccgggga
     5701 cgcccgtgcc gatggcaccg cgctgcaatt gctcgcggct cgagagcttg tcgaccggga
     5761 acttgcgcgg catgcgcggc acgaagtcga ggcagcggac gcgcttgtcc ttggcggaat
     5821 agaccgtcgc gaggccctgg cctgcaaggc tcgacatgta gggctcgacc acgttcagcg
     5881 ccgctgcggt cgcgaccgca gcgtcgaagg cgttgccccc gctcgcgagg agccgcgcac
     5941 ccgcctgcgc ggcgagcgga tgcgccgccg ccaccatgcc gtgcacggaa gtgattgttg
     6001 gtcgccggcc gtacatgttg cctcccgcgt cgctcccggt cccgaacccc caggcgtaca
     6061 cgcccgggac ccgtggcggc aaggcatgtc ggcgcatgac tgcggggcgc cggtcacgga
     6121 gtggctgacg ggcgaggctc agcgggccgc gagctcgcgc cagatcagcc ggagaccgaa
     6181 ggcgacgaag aggccgccga cgactgcctc tgcgccggac cgcatgcggg catagccccg
     6241 gcgcacggcc gcgcgctgga agagccagcc gagcagcgag aggtagctcg cgttcatggc
     6301 gacgatcgca gcgatacagg cgatccctgt cgcgaggctc gcatcgagca cgcgggtcgc
     6361 ggcgaagatg gaggtcagga acacgatcgc ctgcgcgttc gagagcgccg tgccgaagcc
     6421 gaggctgaat gtccgccaga ggggtacgcc cggggcgggt gcagtgtccg gcagaggctc
     6481 gccctctggc gcgttgcggc tccgcagcag gaggcgcacg ccgaaataga tgagataggc
     6541 gccgccgagc acgtgcatgg cgagacgcaa ccaggggaat gtcgcgagca gcgccgccat
     6601 gccgaggagg gccgcgccgg caagcagcac gttgccgacc atgttgccgg cgatcgccat
     6661 ggcgacgtca gcggcgctgc gtgtgagcgc gacgtgcgtc accatcagcg agttggcacc
     6721 aggcgtcggc acaatcacgg cccagagcag gacgaatgcg ccgagcactg caagatcaat
     6781 gttgacgggc atctctgcaa tcgtcccttg gcggttcttc tcgctcagcc cgaggtggca
     6841 ttcgacacgg caacgagcct ttcgagcgcg cgcatggccg cgcccgattc gatcgagtgc
     6901 gcggcgagcg ccgccccgtc cgtgaggttc gccgcggcgc cgccgacgat cagggctgcc
     6961 gccgaattga ggagcacgat gtcgcggaac ggtcccttct tccccgccag cacgtcgcgg
     7021 atcgcggccg cgttcgtctt ggcgtcgcct cccttgagat ccgagagctt ggcgggcgcg
     7081 atgccggcat cggcgggcgt cacctcgaag acgcggatct cgccgccctt cacctcggca
     7141 acgtcggtcg aaccggtcgt cgtcagctcg tcgaggccgt catggccgtg caccacccag
     7201 acatgctcgg cgccgaggtt gcgcaacacg agagcgatcg gctcgaccca ctgccaggag
     7261 aacacgccga cgatctggcg cttcacgccg gccggattgg agatcggtcc gagcaggttg
     7321 aagatcgtgc ggatgccgag ctcggcgcgg accggcgccc agtgcttcat ggccggatga
     7381 tgcatcggcg cccacatgaa gccgacaccg gcctccacga ttgcccgctc gacaacgggc
     7441 ggcgcgacat cgagcttcac gccgagggct gcgagcacgt ccgaggcgcc tgagatcgag
     7501 gagacggagc ggttgccgtg cttggcgatt ttgaggcccg cacctgccgc gacgaggctc
     7561 gcgcaggtcg agacattgta agtgccgtgc gcgtcgccgc cagtcccgac gatgtcaacg
     7621 gcgcccggcg gcgcatgcac gcgcagcatc ttggagcgca tcagctcggc cgccccggtg
     7681 agctcgctca ccgtctcgcc cctcacgcgc agcgccatca ggaaagcgcc catctgcgcc
     7741 tgtgtggcga cgccctccgt catcagctgg aaggcggcgc gggccggaag accatccgcc
     7801 ccctggacat cgaaaaaatc atcgcggaga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactt cgtgatggcc
     8041 gcccccgcac cccgccagcc gcttcagtac ctcgacaagg cggcatccac gcttcgcgat
     8101 ctcggcctga tgccggacaa agtggagccg gcaccgatca atgcgctcct cgagaagatc
     8161 tccgacctcg acaaggacaa gatcaccatc atcgcccgca cgctcgggca ggcggaagtg
     8221 ttcaatgcgg tcgtgcgcga ccagacggcg aagatggagg tcgggcagcg ctaccagcag
     8281 atcacggagg gcttcaactc cattcgcgac gacgccaagc gcctcgtcga ccaggtctcg
     8341 gacggcaagg tcgatctcct cgagcgcggt acaaacatct ggatgaagat cgtacgcggc
     8401 gacatcgccg cccgcttcga cgcgatccgc gccatctatc tctccgtcac caaggagacg
     8461 aagaaccaga tcgagcggga ggggaagatc ctcgacgcct accgcgatta ccgtggcgcc
     8521 ctcaagcact ccgaggtgct ggcgcttgag gtgctgaaga aggccgaggc caagctcgag
     8581 gaagcgaagt cggcactcgg cgaggcttcg cagaaggtcg cgggcttcgc cggcagcgag
     8641 cccgccgaac gcgccaagct cgagctcgca cgcgatgaag tcctgcgccg cacgcaggac
     8701 gaggagaagc gctaccagat cgccaaggac ctctccgaca acctcaccat ctcctacaac
     8761 acgagcgagg tggtgatggc gcgactcatg cagacgacct cggccaagga gcgcatctac
     8821 agccagtcgg tctccttctt ctcgaccaac gacacggtgc tgaccgcgct aaaggcctcg
     8881 ttcaccggca tgttcgggct gcatgagtca acgaagacac tcgatgccat gaaggaaggc
     8941 atgtcgaaga gcatcgagac gatctccgag atcggcgaca aggtgcagga cgccgcgatc
     9001 cgctcgggct acggcccgac gattcgcgcg acatcaaggc cgacgccgtg aagaagctcg
     9061 tcgacagcgt ggtcaatttc caggagcgct cggtcaccat catcgccgag atgcgcaagc
     9121 tcgcgaccga gaacagccgc gagatccgcg acgccgtcga ggatggcaag aagcgcatcg
     9181 ccaagctggt tgcggcagga gacgcgctgc cggggccagg gtgaggtgcg agctagatga
     9241 cgacggaggc cgcgggcgag cccgctccgg gtgccgccaa gtcgcccggc acgctcgacg
     9301 acgtgatgat cgcgatggac gtggtggata cggtccgcca tcgggaggat ctcgtccgcc
     9361 gcgagctcaa cgaggcgggg cgcgaggctg atctcatcgc gcggctccgc gagatctacc
     9421 gccagcaggg catcgaggtg ccggatcacg tgctcgccga aggcgtgaaa gcgctgcgcg
     9481 agagccgctt cacgtacaca cctccgccga agggcctgaa gcgcacgctg ctcacactct
     9541 ggacgcggcg ccgcaccgtc ggtatcggtg cgggcgccgc tgctgcggcg gtggtcggct
     9601 atcagctcgc cattgcgttg ccggcgcgcg aggaagcgag gcggcaggag acggagatca
     9661 cggtcacgct gcccaaggca ttgaaggacg cctacgccga cgtcctcgcc gtctcgaccg
     9721 atccgggcgc caaggggcgc gccgacgcgc tcctcgccga cggcgagcgg gccttgcgcg
     9781 agcgcagtgc cgcggccgcc cgcgagagca acgccaggtt gaaggcgctg cgcgacgagc
     9841 ttgcccagga atacacgctg acgatcgtcg cccgcaaagg cgaggccatg ggcgtctggc
     9901 ggacgcctcc caagagccag acggggcgca actactatct gatcgtcgag gccatcacgc
     9961 cggacggccg cgcgcttccc ctgcccgtgc gcaatgagga gaccggcaag accgagacgg
    10021 tcaccaagct cggcgtgcgc gtcccggagg cgacattcag tgccgcggcg gccgaccgtc
    10081 gcgacgacgg catcgtccag aacaaccgct tcgccatcaa gcggcgcggt gttctcgccg
    10141 tcgagtatct catgcccttc gagggcggca tgatcaccgc ctggtaggag ccctgcgaga
    10201 tgccatccgg ccgccagacg ttgcgcacgc tcgagacctc gatctcgggc atcaaccgcg
    10261 agcaggagac gctcgaggag gcggtgcgcg ccgcctccgc cgaggttgcg cggctccggc
    10321 gcgagcgcat cgcggcgctg cgtgagctcg cgcgggtgaa actcgacgag atggcggccg
    10381 gccggctcac gcgcgatctc gacctcgccg agcggcgggt caccgagatg atggcggcgc
    10441 gcgagcgtgc actcacggag tctgccgagg agcagaagcg ccgcgttgcc gccgtcgaag
    10501 ctgcggaggc gacgcgcgac gaggcggcaa agcgcgtcga ggcgctcgtc aaggaggtgg
    10561 aggacacgcg cctcgccgcc gagacggccg ttgagcgcga gcccgcctgg ctgcaggcga
    10621 acgcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnaagccct atgacgagga tccgctcttc gcctatctct
    10741 ggggacgagg atacctgacg ccgcgctatc atgccggcaa cgtcgcgcgc atgatggatg
    10801 gcctcgtcgc cgagttcatc ggctactcga atgcgcgccc gaactacgtg atgctgagcg
    10861 agatccccgt tcgcctcggc gcccatgcga cctcgcgacg ggccgccgcc gacgagctca
    10921 aagcggcccg cggcgcgatc gaacgccaac gcatggtggc gctcggcatc gagccgaagg
    10981 agcgggcgct tgccgcggca cgggccgagc tgaagacggc cgacaacgcg ctcgaggcc
//
LOCUS       DAORNH010000134        11019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4463_length_11019_cov_2.735316, whole genome shotgun sequence.
ACCESSION   DAORNH010000134 DAORNH010000000
VERSION     DAORNH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11019
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4463_length_11019_cov_2.735316"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PK264_13905"
     CDS             <1..655
                     /locus_tag="PK264_13905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77002.1"
                     /translation="DRTASDETVAAVAAAGGKALGAVVDVASEASTAAMAALAKDRFG
                     RIDGLVNNAALYGSLKGGRFDAISEADWDATMAVNVKGIWNCCKAVVPVMKSSGGGSI
                     VNIASLAATYGMPFALHYTTSKAAVIGLTRGLARELGRDGVRVNAVAPSAVLTEGTRE
                     FFGAKHDKALEVIRGNQTIPRNLESRDVAGAVLWLLSDESAFTTGQTISVDGGTVML"
     gene            779..1813
                     /locus_tag="PK264_13910"
     CDS             779..1813
                     /locus_tag="PK264_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HRD77003.1"
                     /translation="MAKPRPQDAAGQRLHRTLSSPAVYLWRMLIFLMLVGFVVVIIAK
                     PLWTALLTNPFLNGGIVLVLLVGALYAFRQVIRLYPEIRWVNAFRIADPGLAIAHRPV
                     LLTPMATMLRDRTGVLSLSASSMRSIMDSIAGRLDEARDTGRYMVGLLVFLGLLGTFW
                     GLLETIQSVGKAIATLDTKGGDSVTVFDDLKNGLAAPLRGMGTAFSSSLLGLAGSLIL
                     GFLELQASHAHNRFYNELEEWLSGITELTPGSGSSEALNRQLYAAMFDVQRAIAEMSQ
                     RVALPATNTANGAEASVRELAKGVDQLVRQMRAEQKVVREWVDEQAARQSELSTVLRD
                     LSATISRRGG"
     gene            1817..2848
                     /locus_tag="PK264_13915"
     CDS             1817..2848
                     /locus_tag="PK264_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HRD77004.1"
                     /translation="MAGRSRRGRSGDYGEFWPGYVDVLSTLLLTVTFLMSVFMIAQYF
                     VSQESSGKDTALRRLTRQIAELTSLLSLEKGKGRTLEDEIAALQSSLASLKADNARLA
                     GAAGIGDEKAAAAGSRIATLTKDLEEQKLLSGEALAKVDLLNQQLLALRRQMLALQEA
                     LGAAEAKDKDAKLKIDDLDKRLNVALARKVQELQRYRSDFFGRLSELLKDRKDIRIVE
                     DRFVFQSEVLFASGQAQLSPEGLAAMTALAAAIRDLEAQIPPEINWALQVDGHTDKRP
                     IASSAFASNWELSTARAISVVRFFVSQGVSPKRLVAAGYGEFQPLDESDGDLAYQRNR
                     RIELKLTNR"
     gene            3248..3490
                     /gene="purS"
                     /locus_tag="PK264_13920"
     CDS             3248..3490
                     /gene="purS"
                     /locus_tag="PK264_13920"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HRD77005.1"
                     /translation="MKARIKITLKNGVLDPQGKAIQSALGGLGFGGINDVRQGKYIEV
                     DLVETSEAKARDAVDRMCKQLLANMVIENYSYELVS"
     assembly_gap    3558..3567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3947..5107)
                     /locus_tag="PK264_13925"
     CDS             complement(3947..5107)
                     /locus_tag="PK264_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD77006.1"
                     /translation="MPGSSRNPLLGEEHEMLRAQVRRFVETEVKPVAERWETDGFVPR
                     PILRRAGELGFLGLRYPTEYGGSAMDPLASVILGEELGRSTFGGFAITILDHTDMAAV
                     HILHSGSKAQKERWLPRVVDGSAWSAVAITEPDAGSDVKGIRTRARRDGDHWVIDGTK
                     CFITNGVLADVYCVAVKTNPEGGSRSVSMLLVEKGTPGFRVARKFDKMGWRSSDMAEL
                     VFEGARVPAENVIGDEGRGFYALMQNFQNERLVLGAQAVGEMLAALELTLDYVTTRKA
                     FGATLFEKQVVRNRLALLATKVEAARALVYTTAAAYGQGQDCTREVSMVKALCGELVN
                     EVMYQCLQLHGGSGYMTGSVIERMARDARAHPLGGGATDVMLEEVAKRLVRT"
     assembly_gap    5112..5162
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5355..6359
                     /locus_tag="PK264_13930"
                     /pseudo
     CDS             5355..6359
                     /locus_tag="PK264_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772259.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            6444..7205
                     /locus_tag="PK264_13935"
     CDS             6444..7205
                     /locus_tag="PK264_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD77007.1"
                     /translation="MVAARSPVIPPAPLVHARNLPTLRLLVTSARSNLDIWPDYAFDV
                     PFNGRRVLGVESVPLNDPEAIRHVFVTHAANYGRPPLMARVLRGPLGRGIILSEGAEW
                     RRQRRVLAPTFTPASVSGLIPHFQIAAEALLRRLDGKPEADLSVEFQETALEAALLAM
                     FSLPDSEVRVQLGGFVRQFVAGPGRVSMLDCIAPSETSYPLLLRSRRAFQKIWFAARR
                     RSPSAAISSMRCSPPAIPRRAKAPRARRSAIRQRR"
     gene            7130..7780
                     /locus_tag="PK264_13940"
     CDS             7130..7780
                     /locus_tag="PK264_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD77008.1"
                     /translation="MLAARDPETGEGPASEKVRDQAATLIFGGFETTARLLFCASNML
                     ALDPEEQARVRAEVRALRPGSLARLDELDAWPRLRMALLEALRPYPPVPHLVREPRTA
                     DIIKGEAIPAKCQVWASPWVMHRHRAHWRHPTAFMPDRFAGQISPWTTNGAYFPFGAG
                     PRICIAAVFAMAEAEIVLAWLSERYRMSLDDARPALPVGRLTIEPSRAPAFRLERA"
     gene            complement(7806..9452)
                     /locus_tag="PK264_13945"
     CDS             complement(7806..9452)
                     /locus_tag="PK264_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRD77009.1"
                     /translation="MADQKATEDALRESEARLAGILAIAADSIITVDSHHRITLFNDG
                     AEAMFGYRRAEVIGKPLDILLPERARELHRLHVQRFGQSTTTARRMGERQQVYGLRKS
                     GDEFPAEASISRHDFGGRRTYTVILRDITDRRRSEEMLARSRAELEQRVVERTAELSA
                     EIRRREETEAQLVRTQRMEAFGQLTGGIAHDFNNLLTVIIGNLELLEMRLKDERQLAL
                     LSRAREASEMGARLTSRLLTFARRRKYESQVLDLNDQILGMVELLTRSLGDHINLTTR
                     LAPSLWAVRADPSEIENAILNLAINARDAMPKGGELSIETRNVSAEANQIGAITKLAA
                     GDYVRISVSDSGTGMTPDVLRHAFEPFFTTKTPGKGTGLGLSTIYGLVEALGGTATIY
                     SEVDHGTTVSLYLPRDHAEAPAPVSRNVASIPVSAGETVLLVEDNTEVREVTRERLAE
                     LGYIVLEAESGPDAVEQLRSQAAIDVVLSDVVMAGGMSGFDVARAVARDRPGLKTLLM
                     SGYAEDVLRDEGGGSGGLRILRKPFGRAELATALREVLDA"
     gene            complement(9543..>11019)
                     /locus_tag="PK264_13950"
     CDS             complement(9543..>11019)
                     /locus_tag="PK264_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HRD77010.1"
                     /translation="AAPAAPPASDTARALARIDSFPYRHTVGDVMSAPPLWAEPTASV
                     ADALRLLLERRVSSVLVRDQSGAPGIATERDLIRAIARDGAAALTRSVSEVATRPLQS
                     IPVETYVYRAIGRLDRLDFRHLAVADQGGEIIGIVTTRNLLRHRARTAIALGDEIDAA
                     ESVGALAAAWAKVPAVARALIDEDVDPRIISNVISTEVCAMTRRAAEIAANRMRDVGR
                     GEAPCRYAVLVLGSGGRGESLLAADQDNAIVFEHGDPDGSEDRWFAELATVMAEYLDT
                     AGIPFCKGGVMAKSAKWRQSVAGWHATVDTWVRRQKPEDLLNVDIFFDAAVVAGDEGL
                     GDEVIAYAFARAHDARDFQMQMTELARRWQPPFTLFGNFKTGPEGRVDLKLGGLLPIF
                     TAARVLALKHAIRARSTPERLRNFAATTGASETDIETLIGAQGVILKAMISQQLVDIE
                     TGIPPSPRVDLKRLDAGQRAQLKSAVQGVRIATSLVGEGLS"
BASE COUNT         1704 a         3587 c         3812 g         1855 t
ORIGIN      
        1 cgaccgcacg gcgagcgacg agaccgtcgc ggcggtggcg gctgccggcg gcaaggcgct
       61 tggagccgtg gtcgacgtgg cgagcgaggc gtcgaccgca gcgatggctg cgctggccaa
      121 ggaccggttc gggcgcatcg acgggctcgt caacaatgca gccctctatg gctcgctcaa
      181 gggcggtcgc ttcgatgcga tcagcgaggc agattgggac gccaccatgg ccgtcaacgt
      241 caagggcatc tggaactgct gcaaggcggt ggtgccggtg atgaagtcgt cgggcggagg
      301 ctcgatcgtc aacatcgcct cgctggctgc gacctacggc atgcccttcg ccctccacta
      361 tacaacctcc aaggccgccg tgatcggcct cactcgcgga ttggcgcgcg agcttggccg
      421 tgatggcgtc cgcgtcaacg ccgtggcgcc ctccgcggtc ctcaccgagg gcacgcgcga
      481 attcttcggg gccaagcacg acaaggcgct ggaggtcatc cgcggcaatc agacgatccc
      541 gcgcaatctc gaatcgcgtg atgtcgccgg cgccgtcctc tggctgctct cggatgaaag
      601 tgccttcacg accggccaga cgatctcggt cgatggcggc acagtgatgc tctgaacgac
      661 acggcctcat tgccgccgct cgcgggaggt ccgttggtcg cggcgcggca tgcgagcctg
      721 cacttgctcc gccttgttcg ttatgtatgg tcccgcggac gacgggcgga gtgaacgaat
      781 ggcaaaaccg agaccacagg atgcggcggg tcagcggctc caccgtacgt tgagctcgcc
      841 agcggtctat ctgtggcgca tgctcatctt cctgatgctc gtcggctttg tcgtcgtgat
      901 catcgcgaag ccgctctgga cggcgctgct cacgaacccg ttcctcaacg gtggcattgt
      961 gctcgtactg ctggtcggcg ccctctatgc cttccgccag gtgatccggc tctatcccga
     1021 aatccgctgg gtcaatgcct tccgcatcgc cgatccaggc ctcgcgatcg cccacaggcc
     1081 ggtgctgctg acgccgatgg cgaccatgct gcgcgatcgt acgggcgtgc tctcgctgtc
     1141 ggcctcgtcg atgcgctcga tcatggattc gatcgctggc cggctcgacg aggcgcgcga
     1201 caccggacgc tacatggtcg gcctcctcgt cttcctcggc ctgctcggca cgttctgggg
     1261 tctgctcgag acgatccagt ccgtcggcaa ggcgattgcg acactcgata cgaagggcgg
     1321 cgacagcgtc acggtcttcg atgacctgaa gaatggcctt gccgcaccgc tgcgcggcat
     1381 gggcacggcc ttctcgtcgt cgctgctggg cctcgccggt tcgctgatcc tcggcttcct
     1441 cgagctgcag gcgagccacg cgcacaaccg cttctataat gagctcgagg aatggctctc
     1501 tggaattacg gagctgacgc ccggatccgg gtcctcggaa gcactcaacc gtcagcttta
     1561 tgccgcgatg ttcgacgtgc agcgcgccat cgccgagatg tcgcagcgcg tggcactccc
     1621 ggcaaccaat acagcgaacg gcgccgaagc ctccgtgcgc gagctcgcca agggcgtcga
     1681 ccagctcgtc cgccagatgc gcgccgagca gaaggtcgtg cgcgagtggg tcgatgagca
     1741 ggcagcccgc cagtcggagc tctcgaccgt gctgcgcgac ctctccgcga cgatctcgcg
     1801 tcgaggaggc tgagccatgg cgggccggag ccgtcgcggg cgcagcggag actacggcga
     1861 gttctggccg ggctacgtcg acgtgctttc gacgctgctg ctgacggtca ccttcctcat
     1921 gtcggtcttc atgatcgcgc agtatttcgt gagccaggag agcagcggca aggatacggc
     1981 gctgcgccgt ctgacgcgcc aaatcgccga gctcacgagc ctcctgtcgc tcgagaaggg
     2041 caaagggcgt acgctcgagg acgagattgc ggcgctgcaa tcctcgctcg catcgcttaa
     2101 ggccgataat gcgcgccttg ccggggccgc tgggatcggc gatgaaaagg cagcggcggc
     2161 gggctcgcgg attgccacgc tcaccaagga cctcgaggag cagaagcttc tctcgggtga
     2221 ggcgctcgcc aaggtcgacc tgctcaatca gcagctgctc gcgctgaggc gccagatgct
     2281 ggctctccag gaggcgctcg gagctgctga ggccaaggac aaggacgcca agctcaagat
     2341 cgacgatctc gacaagcgcc tcaacgtggc gctcgcccga aaagtgcagg agctgcagcg
     2401 ctatcgctcc gacttctttg gtcggctgag cgagctcctg aaggaccgga aggacatccg
     2461 catcgtcgag gatcgcttcg tcttccagtc cgaggtgctg ttcgcctcgg ggcaggcaca
     2521 gctctcgccg gaagggctcg cggcgatgac cgcgctcgcc gccgccatca gagacctcga
     2581 ggcgcagatc ccacctgaga tcaattgggc gctccaggtg gacggccaca ccgacaagcg
     2641 tccgatcgca tcctctgcct tcgcctcgaa ctgggagctc tcgacggccc gcgctatttc
     2701 ggtcgtccgc tttttcgtgt cgcagggcgt gtcgccgaag cggctcgtcg ccgcaggcta
     2761 cggcgagttc cagccgctcg atgaaagcga cggcgacttg gcctaccagc gcaaccgccg
     2821 catcgagctg aagctcacga accgctagcc tgaccggcca accgagcttt tcgcccgaga
     2881 gcgctcgacg tccgagatgc tgccagcccg cgccgaccgg cgcgcggtta cgcgtggcgg
     2941 cgtgggcaat gtagagacgt gcggagcggc ggtgcctgct cggatgaggc attcttttgg
     3001 gggcgcggca caatttgccg ctccccgtga tgtcgcatcg cggcctgcca gtgtccgtct
     3061 cgacgccgcg acggacactg gcctccgctt cgtggaggag ctgctgcgtc ttctggtgct
     3121 cggcagcggc aagtaggggc aggaccggcc gggtgcggga tggactggca atcgatagtt
     3181 gactgatgct aaagggctgc tctcggaatt cttgtgcagc ccgtttcgtt cgagggagcg
     3241 acaggtcatg aaggcacgca tcaagatcac cctgaagaac ggcgtgctcg acccgcaggg
     3301 gaaagccatc cagagcgcgc tcggcgggct cgggttcggc ggcatcaacg acgtgcgcca
     3361 ggggaagtac atcgaggtcg atctcgtcga gacgagcgag gccaaggccc gcgacgcggt
     3421 cgaccgcatg tgcaagcagc tgctcgccaa catggtgatc gagaactaca gctacgagct
     3481 ggtgagttga gcggcaggcc gcgacacgga acgtcgtctc gagtcaagaa ctgtgggtcc
     3541 gggaggctgt ggctcccnnn nnnnnnnggc gggagcgcga gggcatcccc tcgcccgggg
     3601 aggagctgtg cgacccgatc gagctgctgc tcttgatcgg agcacaacat tactgctttg
     3661 gctcttcggc gaatgggact gtatgaacag ctggaccatg tgccatcggg tcacgtcgat
     3721 tctggcgctc gctccccgcg cgttccggct ggatgcatgg ccccgcctta cggacatttg
     3781 atgcagatgg cccgaatacg agcaaactcg tcccaggccc cgagagggtg tctgccctcg
     3841 ccctcaggat ggaagccacg agtagtaacc cgtgcagcgg agcggatgga cgcgggcgaa
     3901 ccagagttca gtagcttggg gggcggatct ggtcggaccg cgttcctcac gtccggacga
     3961 gccgcttcgc cacctcctcg agcatgacgt cggtcgcgcc gccgccgagg gggtgggcgc
     4021 gggcgtcgcg ggccatgcgc tcgatcactg aaccagtcat gtaacccgag ccgccgtgca
     4081 gctggaggca ctggtacatg acctcgttga cgagctcgcc gcagagcgcc ttcaccatcg
     4141 agacctcccg cgtgcagtcc tgcccctggc cgtaggcggc ggcggtcgtg tacacgagcg
     4201 cgcgcgccgc ctcgaccttg gtcgcaagga gggcgagcct gttgcgcacg acctgcttct
     4261 cgaagagcgt cgcgccgaag gctttgcgcg tcgtcacgta gtcgagggtc agttccagcg
     4321 ccgcgagcat ctcgccgacc gcctgtgcgc cgagcaccag ccgctcgttc tgaaaattct
     4381 gcatgagggc gtagaagccg cggccttcat cgccgataac gttctcggcc ggaacgcgcg
     4441 cgccttcgaa gacgagctcg gccatgtcgg aggagcgcca gcccatcttg tcgaacttgc
     4501 gcgcgacgcg gaagccgggc gtgcccttct cgacgagcag catggagacg gagcggctgc
     4561 cgccttcggg attggttttc accgcgacgc agtagacgtc tgcgagcacg ccattggtga
     4621 tgaagcactt ggtgccgtcg atcacccagt ggtcgccgtc gcggcgcgca cgcgtgcgga
     4681 tgcctttcac gtccgagccg gcgtcgggct ccgtgatcgc gaccgcgctc catgccgacc
     4741 cgtcgacgac gcgcggcagc cagcgctcct tctgcgcctt gctgcctgag tgaaggatgt
     4801 gcaccgcggc catgtcggtg tgatcgagga tggtgatcgc aaagccgccg aaggtcgagc
     4861 ggccgagctc ctcgccgagg atgacactcg cgagcggatc catcgccgaa ccgccgtact
     4921 cggtcggata gcgcaggccc aggaagccga gctcgcccgc gcgcctgagg atcggccgcg
     4981 gcacgaagcc atcggtctcc cagcgctcgg cgaccggctt cacctcagtc tcgacgaagc
     5041 ggcgcacctg cgcgcgcagc atctcatgct cctcgccgag aagcggattg cggctggaac
     5101 ccggcatggc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nngtgcgagg ggtcacccct cgcgcgggtc gaagggcagc agcccttctc gcgaagcgag
     5221 tgccgcgtgg tagggttcgc ttcgcgacgc ggggggcggc gtcgtgccgg aggcgcgctt
     5281 cctgcgcgac gcgaccccgc tggcgggaca gccgccagac cgcccgcctt cttggacatt
     5341 ttgcggagga cgatgtggca gcgctgagag tagggctcgt cgggctcggg atggcagttg
     5401 gcccgcatgc gaaggggctt gccgatctcg cgcgataagg tctgatagag gtggccgcga
     5461 cgttctcgcc gagcgaggcg cggcgcaccg cgttccacaa ggcctacggt tttcctgtgg
     5521 tcgacgatct gcggcgcatt ctcgaagaca gttccatcaa ggccgtgggc atcctgaccc
     5581 cgccgaacac gcatctcgaa ctcgtcacgg ctgctgccgc ggcggggaag cacatcctgc
     5641 tggagaagcc gctcgacatt acgattgggc gggccgaggc gctcgtgaag gccgcggagg
     5701 ccgcggggtg cacgatgggc gtcgtgctgc agcatcgctt ccggccgtcg ggcgagcggg
     5761 cggcggagat cgtccgctcc ggcgcgctcg ggcgcatcgt caactgctcg acctcgatcc
     5821 ggctgtggcg gccacagagc tactacgacg agcccgggcg cggcacgcgg gcgcgcgatg
     5881 gcggcggtgt cctgctcacg caaggcatcc acacgctcga cctgatgctg tcgctggcgg
     5941 ggccaatcgc cgaggcgcgg ggcttcgtca caacgtcgcc ggtgcatcgc atggagacgg
     6001 aggacatggt ggcggctgca gtgcgctacg agagcggcgc catcggcgtc atcgatgcga
     6061 cgacggcggc ctaccctggg gcggccgagc gtatcgatct cgtcggcgag aatggttccc
     6121 tctcgctcgc aggcacgctc ctcgacgtgg cattccacga tggccggcgc gagcagatcg
     6181 cgcccgatgc gacgcccggc ggcacgggcg ccgatccgat ggcgttcccg cacgactatc
     6241 accgcagcgt gtggcgcgat ttcgtgctgg cggtacgaga cggccgcccg ccgcgcgtga
     6301 cgggccgcga ggcgctcaag gtgcaccggc tgatcgagat gctgatcgcc atgcggtgac
     6361 ggccggcgac gttccgaacg tcgcccgtct atgcgacgca gatccgtccc aatgaggacc
     6421 gctggccgtc gaagggatat cccatggttg cagcgcgttc acccgtcatt ccgccggcgc
     6481 cgcttgttca cgcgcgcaat ctcccgacgc ttcgtctgct cgtcacttcg gctcggagca
     6541 acctcgacat ctggcccgac tacgctttcg acgtgccgtt caatggacgc cgcgtgctcg
     6601 gcgtcgagag cgtgccgctg aacgatcccg aggcaatccg gcacgtcttc gtgacgcatg
     6661 ctgccaacta tggccgtccg cctctcatgg cgcgggtgct gcgtggcccg ctcgggcgtg
     6721 gcatcattct ctcggagggc gcggagtggc gccggcagcg acgagtgctg gcaccgactt
     6781 tcacgccggc aagcgtcagt ggcctcattc ctcatttcca gattgcggcc gaggctctac
     6841 tgcgccgcct cgacgggaaa ccggaggccg acctctcggt cgagttccag gaaacggcgc
     6901 tggaggcggc attgctggcc atgttctcgc tgccggacag cgaggtgcgg gtgcagctcg
     6961 gcgggttcgt tcgccagttc gtcgccgggc cggggcgggt cagcatgctc gactgcatcg
     7021 cgccgagcga aacctcctat ccgcttctgt tgcgctcgcg ccgcgcgttc cagaaaatct
     7081 ggttcgccgc gcgtcgcagg tccccgagcg ccgcgatctc ctcgatgcgc tgctcgccgc
     7141 ccgcgatccc gagacgggcg aaggccccgc gagcgagaag gtccgcgatc aggcagcgac
     7201 gttgatcttc ggcggcttcg agaccacggc gcggctcctc ttctgtgcga gcaatatgct
     7261 ggccctcgat cccgaggagc aggcgcgggt gcgagcggag gtcagagcgt tacgtccggg
     7321 ctcgctggcg cggctcgatg agctcgatgc ctggccgcgt ctcaggatgg ccttgctcga
     7381 ggcgctgcgt ccctaccctc cggtgccgca cctcgtccgc gagccgcgca cggccgatat
     7441 catcaagggt gaagcgatcc ctgcgaagtg tcaggtgtgg gcctcgccct gggtgatgca
     7501 tcgccaccgc gcgcactggc ggcatcctac ggccttcatg ccggatcgct tcgcggggca
     7561 aatatcgccg tggaccacga acggggccta tttcccgttc ggcgccgggc cgcgcatctg
     7621 catcgctgcg gtgttcgcga tggccgaggc cgagatcgtg ctggcgtggc tcagcgagcg
     7681 gtatcgcatg tcacttgacg acgcgcgtcc cgctctcccg gtcggccgcc tgacgatcga
     7741 gccgagccgg gcgccggcat ttcggctgga gcgggcgtag cgacgcccga gatgccgact
     7801 gggcatcagg catcgagcac ctctcgtagc gcggtcgcga gctcggcccg gccgaagggc
     7861 ttgcgcagga tgcgcagacc gcccgacccg ccgccctcat cgcgcagcac atcctccgcg
     7921 tagcccgaca tcaggagcgt cttcagtcca ggcctgtcgc gggcgacggc ccgggcgacg
     7981 tcgaaccccg acatgccgcc cgccatcacg acgtcgctca ggacgacatc gattgcggcc
     8041 tgcgatcgca gctgctcgac ggcatcggga ccgctctcgg cctcgagcac gatgtagccg
     8101 agttccgcga ggcgctcacg cgtcacctcg cgcacctcgg tgttgtcctc gacgaggagg
     8161 actgtctcgc cggcggacac gggaatgctc gcgacattac gtgatacggg ggctggtgcc
     8221 tccgcatgat cgcgcggtag atacaggctg acggtggtgc cgtggtcgac ctcgctgtag
     8281 atggtcgccg tgccgccgag cgcctcgacg agaccgtaga tggtggagag cccgaggccg
     8341 gtccccttgc caggtgtctt ggtcgtgaag aacggctcga acgcgtgtcg aagcacgtcg
     8401 ggcgtcatgc cggtaccgct gtcggagacg gaaatgcgca catagtcgcc ggcggcaagc
     8461 ttggtgatgg cgccgatctg gttggcctcg gcgctgacgt tgcgcgtctc gatgctgagc
     8521 tcgccgccct ttggcatggc gtcgcgcgcg ttgatggcga ggttcaggat ggcgttctcg
     8581 atctcgctgg ggtcggcccg cacggcccac aggctcggtg cgagacgggt cgtcagattg
     8641 atgtgatcgc cgagcgagcg cgtcagcaac tcgaccatac cgaggatctg gtcattgagg
     8701 tcgagcacct ggctctcgta tttgcgccga cgggcgaagg tgagcaggcg cgaggtgagg
     8761 cgggccccca tctcgctcgc ctcccgcgcg cggctgagca gcgccagctg cctctcgtct
     8821 ttgaggcgca tctcgagcag ctcgaggttg ccgatgatga ctgtgagtag gttgttgaag
     8881 tcgtgcgcga tgccgccggt gagctgaccg aaggcctcca tgcgctgggt gcgcacgagc
     8941 tgggcctcag tctcctcgcg gcggcggatc tcggcgctga gctcggccgt gcgctcgacg
     9001 acgcgctgct cgagctcggc gcggctgcgg gcgagcatct cctcggagcg gcggcggtcc
     9061 gtgatgtcgc gaaggatcac ggtgtaggtg cgccggccgc cgaagtcatg ccgggaaatg
     9121 gaggcttcag ccgggaattc gtcgccgctc ttgcgtagcc catagacctg ctggcgctcg
     9181 cccatgcggc gggccgtcgt cgtcgactgg ccgaagcgct gcacgtgcag gcgatgcaac
     9241 tcgcgtgcgc gctcaggaag caggatgtcg agcggctttc cgatgacctc ggcgcgccgg
     9301 tatccgaaca tcgcctcggc gccatcgttg aagagcgtga tgcgatggtg gctgtcgacc
     9361 gtgatgatgg aatcggcggc aatggcgaga atgccggcga ggcgcgcctc ggattctctt
     9421 agtgcgtcct cggttgcctt ctgatcggcc aagcgcggct cccggactgt gcggaccgtg
     9481 aggtgtactc taatcagggt tgcgccgaat gctcaaccgc agcggcacac ctcaattccg
     9541 tatcagctca agccctcacc gacgagcgag gtggcgatgc gcacaccctg gactgcgctc
     9601 ttcaactgcg cacgctggcc ggcatcgagg cgtttgagat cgacgcgcgg ggagggcggg
     9661 atgccggtct cgatgtcgac gagctgctgc gagatcatgg ccttgaggat cacgccctga
     9721 gcgccgatca gggtctcgat atcggtctcc gaggccccgg tcgtggcggc aaaattgcgg
     9781 agccgctcgg gtgtgctgcg ggcgcgaatg gcatgtttga gggcgaggac gcgggccgcc
     9841 gtgaagatcg gcaggaggcc cccgagcttg aggtcgacgc ggccttctgg gcctgtcttg
     9901 aagttgccga agagcgtgaa gggtggctgc cagcgccggg cgagctccgt catctgcatc
     9961 tggaagtcgc gggcgtcgtg agcgcgagcg aaggcatagg cgatcacctc gtcgccgagc
    10021 ccctcgtcgc cggccacgac cgcggcgtcg aagaagatgt cgacgttgag gaggtcttcg
    10081 ggcttctggc ggcgcaccca agtgtcgacc gtcgcgtgcc agccagcgac gctctgccgc
    10141 cacttcgcgg acttggccat cacgccaccc ttgcagaagg gaatgccggc cgtgtcgaga
    10201 tactcagcca tgacggtcgc gagctcggcg aaccagcggt cctccgagcc atccggatcg
    10261 ccgtgctcga agacgatggc gttatcctgg tcagcggcaa gcaggctttc gccgcgacca
    10321 cccgagccga ggacgagcac ggcgtagcgg cagggcgcct cgccgcgccc gacgtcgcgc
    10381 atgcggttcg ccgcaatctc ggcggcccgc cgcgtcatgg cgcacacctc ggtgctgatg
    10441 acgttggaga tgatgcgcgg gtcgacgtcc tcgtcgatga gtgcgcgggc gacggccggc
    10501 accttggccc aagcagcggc gagcgcgccc acgctctcgg cggcgtcgat ctcgtcgccg
    10561 agcgcgattg ccgtgcgagc ccgatggcgg agcaggttgc gcgtcgtcac gatgccgatg
    10621 atctcaccac cctgatccgc gacggcgaga tggcggaagt cgagccggtc gaggcggccg
    10681 atggcccggt aaacgtaggt ctcgacggga atgctctgca gcggccgggt tgccacctcc
    10741 gagacggatc gcgtgagcgc cgccgcgcca tcgcgggcaa tggcacggat gaggtcacgc
    10801 tcggtcgcga tccccggtgc gccggactga tcgcggacga gaaccgagct gacgcggcgc
    10861 tcgagcagga ggcggagagc gtcggcgaca ctcgccgtcg gctcggccca gagtggcggc
    10921 gcgctcatca catcgccgac cgtgtggcgg taggggaagc tgtcgatgcg ggcgagggcg
    10981 cgcgccgtat ctgaggccgg tggcgccgcg ggggcggcg
//
LOCUS       DAORNH010000135        10953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4487_length_10953_cov_2.744907, whole genome shotgun sequence.
ACCESSION   DAORNH010000135 DAORNH010000000
VERSION     DAORNH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4487_length_10953_cov_2.744907"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /gene="rnr"
                     /locus_tag="PK264_13955"
     CDS             <1..1785
                     /gene="rnr"
                     /locus_tag="PK264_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRD77011.1"
                     /translation="ALGIFRAHARGGGEIAPIDRRALRSISVRVGNTGGAEDGDLVRY
                     DLTRTGRFGTSDAIVAERLGNPGDQRQVSLIAIHANGIPDTFPGAVLREADALSAALL
                     DGRVDLRRTPLITIDPADARDHDDAVYAEADTDAANPGGHVVIVAIADVAHYVRTGTR
                     LDREAELRGNSVYFPDRVVPMLPERISNDLCSLREGEDRACLAVRMTFSARGRKLRST
                     FMRGLMRSRAKLAYEEAQAAIDGRPSEKGAALLDDVLKPLWAAYKALLMAREERAPLD
                     LDLPERKILIGKDGRVARVVVPERLVAHRLIEEMMIQANVAAAEALEERRTPCVYRVH
                     DKPDRERLDALRDFLASLDLTLPKASELTPAHINTMLARAREMPVADLVNEVVLRSQA
                     QAVYGTENIGHFGLNLSRYAHFTSPIRRYADLLVHRALIRALKFGSDGLAVESEPRLP
                     DIARAISMTERRAMAAERETADRLIAAYLADRIGAEFEARVSGLTRGGLFVRLKETGA
                     DGFIPAETIGGDYWRHDEALHALIGDRTGLAFRLGDDVSVRLVEVVATAGAMRFEMLS
                     DGRRLREGGYATRRRGHRLPGRQRSRRA"
     gene            1952..2275
                     /locus_tag="PK264_13960"
                     /pseudo
     CDS             1952..2275
                     /locus_tag="PK264_13960"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
     gene            2322..2981
                     /locus_tag="PK264_13965"
     CDS             2322..2981
                     /locus_tag="PK264_13965"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRD77012.1"
                     /translation="MIPPYGRRVLLVEEPIAASLGAGMAIDEPAGSMVLDIGGGTSDI
                     GVMSLGDVVQARSLRCAGNAMDEAIVRHVRRKHELKIGIANAERIKIEAGTASVTPAV
                     EQAEIHIRGRDLREGRPKTVVLGPADIAEALDAPIEEIADFVQRTLEDLPPEVSTDIC
                     ERGITLTGGGALLDQLSFELERRVGVPFTVPDNPMQCVVRGAAKLLDSLKIREHLLIK
                     P"
     gene            3131..4063
                     /gene="mreC"
                     /locus_tag="PK264_13970"
     CDS             3131..4063
                     /gene="mreC"
                     /locus_tag="PK264_13970"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRD77013.1"
                     /translation="MSSLAKDRFARRAVQDKPRSARTSVMVFMLVAAALLASSQIDHP
                     LLAAARRHASDLLTPALTAANALTEPVRALGRQLRSHSSLVAEVERLKGEVQRLSAWE
                     ARAKELERRIGHLTALARVVDEPAVGFVTVRVIVEARSPFVRTALVGAGRNHGLRPGY
                     PVINGDGLVGRIVDAGDRSARLLLLSDPASRIPVIVGAASVDAILLGGGGSSARLTLF
                     ANGAKVSDGDEVVTSGVGGLFPRGLRIGVARQVAADLTVELYAHLDQLDYVSVLQFQS
                     PLLDIIDRDRVAASALPRNFASGAVGGPGAEPGK"
     gene            4089..4616
                     /locus_tag="PK264_13975"
     CDS             4089..4616
                     /locus_tag="PK264_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77014.1"
                     /translation="MIARAAAAIAFLSITLLSAVSWGVPSAWHLVPVMIPFAYIHAAA
                     HEPRAALGAAATFAGGVLTDTLTDGPLGFWALLYLVGYLASTQSATLVSDWRGNRPMS
                     PLVAMLTAVPVASVVVAAIAAAATLRDPGTMSSGGALRSIIAMALASGLIGVGMNAAL
                     SRRRNDQAPGHPERA"
     gene            4621..6441
                     /gene="mrdA"
                     /locus_tag="PK264_13980"
     CDS             4621..6441
                     /gene="mrdA"
                     /locus_tag="PK264_13980"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRD77015.1"
                     /translation="MADSPRDEATSRRTFTRRALMLGLGQTGALGLLGARLYHLQVTE
                     REHYARLAEANRTRTLAIPALRGRILDRTGRVLAGNETSIRVVVVPALAGRLQDVLRA
                     LARLVPLGEDEQAAIVDRARRQPPNLPVVVADEIAFEDAARINLLSSDLPGVLTEHFA
                     RRVYHGGAAVNHIVGYVGAPERPALGDDPVLRHPDVRVGKTGAELGLETTLRGHAGRI
                     RREVDARGRIVRSFDDGRAITGTDVTLTIDLEVQKRVMQRMSQERRAALVALDCREGE
                     IVAMASTPGYEHSDLSSPGRGTTEKSNSRLADEPFIDRTIRGLYPPGSTYKIVTALAA
                     LESGAVAPDEPLPCSGAFELAGHTFNCWKRSGHGSSNLVRALAQSCDCYFYEAARRTG
                     IERLAATARLLGFGRTYDCGLALQRSGIVPDPDWKRGRYGRSWVTGETVLAGIGQGFV
                     LTNPLQLAVMIARAASGLAIEPTLVIARPEDARGDALPLPVERRHLDLVRRGLVAAVN
                     EDGGTGASARLDQEMGELAGKTGTAQVKRFSAATRWTERDHSLFVGYAPVAAPRYAIA
                     AIVEHAGSGGAVAAPLVREVMQFLFDRDAPPGKPVGSRRS"
     gene            6445..7596
                     /gene="rodA"
                     /locus_tag="PK264_13985"
     CDS             6445..7596
                     /gene="rodA"
                     /locus_tag="PK264_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRD77016.1"
                     /translation="MGQHSSGGQLPATIAERLVAVNWPLLALVALIAIVGIAQLQSAA
                     GGSMSPWADKQAWRLVFGLALVILLAILPIRLWIGLAYPAYAAGLVLLVLVVVAGNEA
                     LGARRWLKLGELSLQPSEVMKIGLVLALARYYLWLPPERVSQPLWVAIPLVLIVIPVA
                     LVLTQPDLGTATLFAAVGFALMFLAGVSMLYFLGGAMGLAIASPLIWMGLHDYQRRRV
                     TTFLSPDADPLGAGYHITQSKIAVGSGGWSGLGHLGGLQSHLDFLPEKHTDFAFAMFA
                     EEWGFVGAASLLALYGVLIAMLIAMALACRSGFSRIVIAGTCISISIYVVINVAMVTG
                     LVPVVGVPLPLVSYGGTAMITVMISLGLAMCAWVNRRAVIRPDELRALI"
     gene            complement(7629..8141)
                     /gene="lspA"
                     /locus_tag="PK264_13990"
     CDS             complement(7629..8141)
                     /gene="lspA"
                     /locus_tag="PK264_13990"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRD77017.1"
                     /translation="MSSSTNAQPPSAYLIYGLAIALAALFVDQAHKWAMIKGLALPEG
                     QRFQLAPFLDIIYVRNTGISYGWLSQSSTGGQWALAGFAVLAAIGLSLWLARGVSNRL
                     LAASVGLIIGGAMGNAIDRVVLGGVADFFSLHAFGYYWYVFNIADVAIVAGVVGMLWE
                     SIIGPRMPGR"
     gene            8319..8723
                     /locus_tag="PK264_13995"
     CDS             8319..8723
                     /locus_tag="PK264_13995"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HRD77018.1"
                     /translation="MTTAFLSALLAFLGYLVLACALMSVFALTYMRSTPHDELRHIRE
                     GNLAATLGFVGALIAYAIVLSRAITVSHGLLETLVWGLIGLVVQVAAHFILSKVMPQL
                     YSSLEQGQMSAGLMQGSLAISLGLINAASMTP"
     gene            8720..9154
                     /locus_tag="PK264_14000"
     CDS             8720..9154
                     /locus_tag="PK264_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77019.1"
                     /translation="MNEATPRRRSTQVKLVLVGTGSLLAVGAGGCGDKGTFHRNVYAS
                     LEDCARDYRPDQCERGGAASLATGVVTPGAAVKGPVYRMLAGRPSACTSTDPGAGRSW
                     NGFAGAGRIGVEAVPRGGFGVACRSSRRTSSTHSSSSWRWGS"
     gene            9154..>9353
                     /locus_tag="PK264_14005"
     CDS             9154..>9353
                     /locus_tag="PK264_14005"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HRD77020.1"
                     /translation="MHRVACPERADWQARIGALGVDLAASPQSPYWREDAYYRFSPSE
                     IDALHDAAAEIERLICAAVDHVV"
     assembly_gap    9354..9492
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9493..10267
                     /locus_tag="PK264_14010"
     CDS             <9493..10267
                     /locus_tag="PK264_14010"
                     /inference="COORDINATES: protein motif:HMM:NF015683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HRD77021.1"
                     /translation="TSLFEAAVVQWHWLEEGHAELDQYNSIHEALIETWRNWRRDGRM
                     SRVHFACFPEDDDDLLTTAYLMDTALQAGLDGVMLEVPEIGWDGRNFVDLEGRRIDAL
                     FKLYPWDWMAADPFFAHIAASGVRMVEPAWRVIASSKALLAVLWERFPDHPNLLAASM
                     RHSLIERPRVLKPILGREGANVVIETAAGPVTRAEGPYAEMAAIAQTLAPLPEYGGWH
                     PVVGVWMVGGVPHGAGVREDRSAITGRGARFVPHVIAGG"
BASE COUNT         1634 a         3634 c         3653 g         1893 t
ORIGIN      
        1 gcgctcggca tcttccgcgc gcatgcccgc ggcggcggcg agatcgcgcc gatcgaccgg
       61 cgggcactca ggagcatctc ggtgcgcgtc ggcaacacgg ggggcgcgga agacggcgat
      121 ctcgtgcgct atgacctcac gcgcactggc cgcttcggga cgagcgacgc gatcgtcgct
      181 gaacggctcg gcaatccggg cgatcagcgc caggtgagcc tgatcgccat ccatgcgaac
      241 ggcattccgg acacgttccc tggagccgtg ctccgcgagg cggacgcgtt gtcggcggcg
      301 ctgctggacg gccgcgtcga cctccgccgc acgccgctca tcacgatcga tccggccgac
      361 gcgcgcgacc acgacgatgc cgtctacgcc gaagccgaca cggacgcagc caatcccggc
      421 ggacatgtcg tgatcgtcgc catcgccgac gtcgcgcact atgtgcggac aggaacgcgc
      481 ctcgaccggg aggctgagct gcgcggcaac tcggtctatt ttcccgatcg cgtcgtgccg
      541 atgctgccgg agcgcatctc gaacgacctc tgctcgctcc gcgaggggga ggaccgcgcc
      601 tgccttgccg tgcgtatgac gttcagcgcg cgcggtcgca agctcaggtc gaccttcatg
      661 cgcggcctga tgcgctctcg cgccaagctc gcctacgagg aggcgcaggc ggcgatcgac
      721 gggcggccgt cggagaaggg cgcggccctc ctggacgatg tcctgaagcc gctgtgggcc
      781 gcctacaagg cgctgctcat ggcccgcgag gagcgtgcgc ccctggacct cgatcttccg
      841 gagcgcaaga tcctgatcgg caaggacggc cgcgtcgccc gcgtcgtcgt ccccgaacgc
      901 ctcgttgccc atcgcctcat tgaggagatg atgatccagg cgaatgtcgc ggcagcggag
      961 gcactcgagg agcggcgtac gccctgcgtc taccgcgtac acgacaagcc cgaccgcgag
     1021 cggctcgacg cgctccgtga tttccttgcg agcctcgatc tgacactgcc gaaggcgtcc
     1081 gagctgacac cagcgcacat caacaccatg ctggcgcggg cccgcgagat gccggttgcc
     1141 gatctcgtca acgaggtggt gctgcgatcg caggcgcagg ccgtctatgg aaccgagaac
     1201 atcggccact tcgggctcaa cctcagccgc tacgcccatt tcacctcgcc gatccgccgc
     1261 tatgcggatc tactcgtgca tcgcgcgctc atccgggcgc tgaagttcgg cagcgacggc
     1321 ctcgccgttg agagcgagcc gcggctcccc gacatcgcca gggcgatctc gatgaccgaa
     1381 cggcgcgcca tggccgccga gcgcgagacc gccgaccggt tgatcgcggc atacctcgcc
     1441 gaccggatcg gcgccgagtt cgaggcgcgt gtctcgggcc tcacgcgcgg cggcctgttc
     1501 gtcaggctga aggagaccgg tgcggacggc ttcattccag ccgagaccat cggcggcgac
     1561 tactggcgcc acgacgaggc gcttcacgcc ctgatcggcg accggacagg gctcgcgttc
     1621 cgcctcggcg acgacgtcag tgtgcgcctt gtcgaggtgg tggcgaccgc gggcgcgatg
     1681 cgtttcgaga tgctgagcga cggccgccgg ctccgcgagg gcggctatgc cacgcgccgg
     1741 cgtggtcatc gcctgcctgg acggcagcgt tcgcgccgcg cctgaccggc ggcatgctcc
     1801 ggacgacgat gatccttgtc cgaccggtga cggtgtgccg tcccggctcg cttgcctgtg
     1861 aattaagaat ttcgcgcaga gccgagttgc agccgaattc ttgctgctat aacgacccct
     1921 tccggctcgg ccggcgactg gggcaaggtc aatgcttgag cgtgtcatcg gcgcgttctc
     1981 ggacgatatc gcgatcgatc tcggcaccgc gaacacgctg gtctatgtga agggccaggg
     2041 catcgtcatc gacgagccgt cggtcgtggc gatccgcgcc cgtggcggca cgcgcgaggt
     2101 cctggcggtc ggccagaagg ccaagatgat gattggccgg acgccggaat cgattgagac
     2161 gatccggccg ctccgcgacg gcgtcatcgc cgatttcatc gcgaccgagg agatgctgcg
     2221 ccagttcatc cgccgcacgc gctcgctcat gggctttcgc cgtccgcgca ttctcatttg
     2281 cgtgccggcg ggcgcgacgc ccgtcgagcg gcgcgccgtc tatgataccg ccgtatgggc
     2341 ggcgcgtgct gctcgtcgaa gagccgatcg ccgcctccct cggcgccggc atggcgatcg
     2401 acgagccggc gggctccatg gtgctggaca tcgggggcgg caccagcgac atcggcgtca
     2461 tgtcgctcgg cgacgtggtg caggcccgct cgctgcgctg cgccggcaac gccatggacg
     2521 aggcgattgt gcgccatgtc cggcgcaagc acgagttgaa gatcggcatt gccaatgccg
     2581 agcgcatcaa gatcgaggcc ggcacggcat cggtgacgcc cgccgtggag caggccgaga
     2641 tccacatccg cggtcgcgac ctgagggagg gccggcccaa gacagtcgtg ctcggcccgg
     2701 ccgacatcgc cgaggcgctc gatgccccga tcgaggagat cgccgacttc gtccagcgca
     2761 ccctcgagga cctgccgccc gaggtctcga ccgacatctg cgagcgcggc atcacgctga
     2821 ccggcggcgg cgcgctgctc gaccagctga gcttcgagct cgagcgccgc gtcggagtac
     2881 cgttcacggt gcccgacaat ccgatgcagt gcgtggtccg gggggccgcc aagctcctgg
     2941 atagcttgaa gatacgcgag catctgctta tcaagccata ggattggttc tcgcaccttg
     3001 gtcggcctcg gtgccgtggg gccaatcaga gttgtgaggg tcgccgcagg ccggcggcaa
     3061 tcgtcgtgcc gtagagcgaa cggatcgatg ccgcgagtgt gggatcgacg ccggagaagg
     3121 tagcagcacc atgtccagcc tggcgaaaga tcgctttgcc cgccgggcag tgcaagacaa
     3181 accgcggtcc gcgcgtacga gcgtgatggt gttcatgctc gtcgctgccg cactcctggc
     3241 ctcgagccag atcgaccacc cgctcctcgc ggcggcgcgg cggcatgcca gcgatctgct
     3301 gacgccggcg ctcaccgccg ccaatgcgct cacggagccg gtacgagcgc tcggccggca
     3361 actcaggagc cattcctcgc tcgttgcaga ggtcgagcgg ctcaagggtg aggtccagag
     3421 gctcagcgcc tgggaggcgc gcgccaagga gctcgagcgc cggatcgggc atctcacggc
     3481 gctcgcgcgc gtcgtcgacg agccggcagt cggctttgtc accgtccgcg tgatcgtcga
     3541 ggcccgtagc ccgttcgtgc ggaccgcact cgttggggcc gggcgcaatc acgggctcag
     3601 gcccggctat ccggtcatca acggtgacgg gttggtcggg cggatagtcg atgctggcga
     3661 caggtcggcg cgcctcctcc tcctcagcga ccccgcgagc cgcattccgg tgattgtcgg
     3721 cgccgcgagc gtcgacgcga tcctgctcgg tggcggtggc agctccgcgc gcctgaccct
     3781 tttcgccaat ggcgcaaagg tctccgacgg cgacgaggtc gtgacgtcgg gggtcggcgg
     3841 attgttcccg aggggcctgc gcatcggcgt ggcgcgacag gtcgcagctg acctcaccgt
     3901 cgagctttac gcgcatctcg atcagctcga ctatgtgagc gtcctccagt tccagtcgcc
     3961 gctgctcgac atcatcgatc gcgaccgcgt cgcggcgagc gcgctcccgc gcaacttcgc
     4021 gagcggagct gtgggcggac cgggcgccga accgggcaaa tgaaccgccg agatgagagc
     4081 gccaggacat gattgcccga gcggcagctg ccatagcctt tctgtcgatc acattgctct
     4141 cggcggtgag ctggggcgtg ccctctgcct ggcaccttgt gccggtgatg atcccgttcg
     4201 cctacattca tgcggcggcc cacgagcctc gcgccgcact cggcgccgcc gcgacttttg
     4261 cgggcggggt gctcaccgat accctgaccg acggcccgct cggcttctgg gcgctcctct
     4321 acctcgtcgg ctacctcgcc tcaacgcaga gcgcgacact tgtctcggat tggcgcggca
     4381 atcgaccgat gagcccgctc gtcgccatgc tcaccgccgt gccggtcgcc tccgtggtgg
     4441 tcgccgcgat cgctgcggcg gcaacgctcc gcgaccctgg caccatgtcg tccggcgggg
     4501 cactccggtc cattatcgcg atggcgctcg caagcgggct cataggagtc ggtatgaacg
     4561 ccgcgctttc acggcgccgc aatgaccagg cgcccggtca tccggagcga gcctgacgcc
     4621 atggccgaca gtccacgaga cgaggcaaca agccgccgca ccttcacgag gcgcgccctg
     4681 atgctcgggc tggggcagac cggcgccctc gggctgctcg gcgcgcggct ctatcatctc
     4741 caggtcacgg agcgcgagca ctatgcgcgg cttgccgagg cgaaccgaac gcgcacactc
     4801 gccattccgg cgttgcgtgg tcgtattctc gatcgcacgg ggcgagtcct cgccggcaac
     4861 gagacttcca tccgcgtcgt tgtcgttccg gcgttggccg gcaggctcca ggacgtgctt
     4921 cgcgcgctgg cgcggctcgt gccgctcggc gaggacgagc aggccgctat cgtcgatcgt
     4981 gcgcgccgcc agccgccgaa cctgccggtc gtcgtcgcgg atgagatcgc cttcgaggac
     5041 gcggcccgca tcaacctgct gagcagcgac ctgcctggcg tgctgaccga gcactttgct
     5101 cgccgcgtgt atcatggcgg cgccgctgtc aatcatatcg tcggctacgt cggtgccccc
     5161 gagcggcctg ctctcggcga cgatccagtg ttgcgccatc ccgatgtgcg tgtaggcaag
     5221 acaggggccg agctcggcct cgagacgacg ctcaggggcc atgcggggcg catccgtcgc
     5281 gaggtcgatg cccgcggccg catcgtaagg agcttcgacg acggccgcgc catcaccggc
     5341 acggacgtca cgctgacgat cgatctcgag gtgcagaagc gtgtgatgca gcgcatgtcg
     5401 caggaacggc gggcggcatt ggtcgcactc gactgccggg agggcgagat cgttgccatg
     5461 gcctcgacgc ccgggtacga gcacagtgac ctgtcgagcc ccggccgtgg cacgacggag
     5521 aaatccaatt ccaggctagc cgacgagcca ttcatcgatc ggacgatccg cggcctctat
     5581 ccgcccggct cgacctacaa gatcgtgacg gcgctggcgg cactcgagtc gggtgccgtc
     5641 gcgcccgacg agccgcttcc ctgttcgggt gccttcgaac tcgcgggcca caccttcaat
     5701 tgctggaagc gcagcggtca cggctcgagc aacttggtgc gcgcgctcgc ccaatcctgc
     5761 gactgctatt tctacgaagc ggcccgacgg accggtatcg agcgcctggc cgctacggcg
     5821 cgcctcctcg gcttcggccg cacctacgac tgcgggctcg cgctgcaacg gtccggtatc
     5881 gtccccgatc ccgattggaa gcgtggccgc tatgggcgat cctgggtgac aggcgagacc
     5941 gttctcgctg gcatcgggca gggcttcgtg ctgaccaacc cgctgcaact cgccgtcatg
     6001 attgcgcggg cagcgagcgg cctggcgatc gagccgacac tcgtgatcgc gcgcccggaa
     6061 gatgctcggg gcgatgccct gccgctcccg gtcgagcgtc gccatctcga tctcgtccgt
     6121 cgcggtctgg tggcggccgt caacgaggac ggcggtacgg gtgcatcggc gaggctcgat
     6181 caggagatgg gagagcttgc gggaaaaacc ggcacggcgc aggtcaaacg gttctccgcc
     6241 gcgacccgct ggaccgagcg tgaccactcg ctcttcgtcg gctatgcgcc ggtcgcggcg
     6301 ccgcgatacg cgattgcggc gatcgtcgag catgcgggca gcggcggggc tgttgccgcc
     6361 ccgcttgtgc gcgaggtgat gcagtttctc ttcgatcggg atgcgccgcc cggaaagccg
     6421 gtcggatcgc ggaggagctg agtgatgggc cagcattcgt cagggggcca gctgccggcg
     6481 actatcgccg agcgcctcgt cgccgtgaac tggccgctgt tggcgctcgt cgccctgatc
     6541 gcgatcgtcg gcattgcgca gcttcagtca gctgccggcg gctccatgtc gccctgggcc
     6601 gacaagcagg cctggcggct cgtcttcggc ctcgcccttg tcatcctgct ggcgatcctg
     6661 ccgatacggc tgtggatcgg gctcgcctac cccgcgtatg cggctggcct cgtcctcctc
     6721 gtgctcgtcg tcgtcgcggg caacgaggcg ctcggtgccc gccgttggct caagctcgga
     6781 gagctcagtc tgcagccgtc ggaggtcatg aagatcggcc tcgtcctggc gctggcgcgc
     6841 tactatctct ggctgccgcc cgagcgggtg tcgcagccgc tctgggtcgc gatcccgctg
     6901 gtcctgatcg tgattccggt ggcgctcgtg ctgacgcaac cggacctcgg cacggcgacg
     6961 ctgtttgcgg cggtcggatt tgcgctgatg tttctcgcgg gcgtgagcat gctctatttt
     7021 ctcggcggcg cgatggggct cgcgatcgcc tccccgctga tctggatggg tctccacgac
     7081 taccagcggc gccgcgtcac cacgtttctt agtcccgatg ccgacccact cggcgcgggc
     7141 taccacatca cgcagtctaa gattgcggtc gggtcgggcg gctggtcggg gcttggccat
     7201 ctgggtggcc tccagagcca cctcgacttc ctcccggaga agcacaccga ctttgcattc
     7261 gccatgttcg ccgaggaatg gggattcgtc ggcgcggcga gcctgctcgc cctctacggg
     7321 gtgctgattg ccatgctgat agccatggcg ctcgcctgcc gctccggctt ctcgcgaatc
     7381 gtgattgcag gcacgtgcat ctcgatctcg atctacgtcg tcatcaacgt cgctatggtg
     7441 acgggcctcg tgccggtggt cggggtgccg ctgccgctcg tctcctatgg cggtacggcg
     7501 atgatcaccg tcatgatatc gctcgggctc gcgatgtgtg cctgggtgaa ccggcgcgcg
     7561 gtcatccggc ccgacgagct ccgcgcgctc atctgagcct tgcggaggtg cgtgccggcg
     7621 tcatgaaact accggcccgg catccgtggc ccgatgatgc tttcccataa catgccgacc
     7681 acgccagcga cgatcgccac gtcggcaatg ttgaacacgt accagtagta tccgaacgcg
     7741 tgcagcgaga agaagtccgc gacaccgccc agcacgacgc ggtcgatggc attgcccatg
     7801 gctccgccga tgatgaggcc gacgcttgcc gcaagcaggc gattgctgac accacgcgca
     7861 agccacagcg agaggccgat cgctgccaga acggcgaagc ccgcaagcgc ccactgcccg
     7921 cccgtcgagc tttgcgagag ccagccgtag ctgatcccgg tattgcgcac gtagatgatg
     7981 tcgaggaacg gggcgagctg gaagcgctga ccctctggca gcgccaaccc cttaatcatg
     8041 gcccacttgt gcgcctgatc gacgaacagc gcggccaacg cgatggcgag gccgtagatc
     8101 aagtaggccg acggcggctg ggcgtttgtc gaggagctca tggcgacgca tgtaggctat
     8161 ccagcgttgc cgtcaagcat gccgatgcaa gccatcgcgc tcatgtcgcg cggtttgtcg
     8221 agaaacctcg accgacgctt tggcttgaat ccattcacct ttgccggata ccctcaaaga
     8281 cggcgtgtgc cgaagagtgc ttttcatccg gagatcccat gacgacggcc ttcctcagcg
     8341 cactccttgc cttcctcggc tacctcgtcc tggcatgtgc gctcatgagt gtttttgccc
     8401 tgacctacat gcgttcaacg ccgcatgacg agctgcgcca catccgtgaa ggcaatctcg
     8461 ccgcaacgct cggcttcgtc ggggccttga tcgcctatgc gatcgtgctg agccgcgcca
     8521 tcaccgtttc gcacgggttg ctcgagacgc tggtgtgggg cctgatcggc ctcgttgtgc
     8581 aggttgcggc gcatttcatc ctcagcaagg tgatgccgca actctattcc tcgctcgagc
     8641 aaggtcagat gtcggctggg ctgatgcagg gtagccttgc catttcgctc ggcctcatca
     8701 atgcggcgag catgacgcca tgaacgaggc gacgccccgc cggcgcagca cacaggtgaa
     8761 gctcgtcctc gtcgggacgg gttcactgct ggcggtcggc gccggcggtt gtggcgacaa
     8821 gggcaccttc catcgcaacg tctatgcctc gctcgaggat tgtgcccgcg actatcggcc
     8881 ggaccagtgt gaacgcggcg gagctgcgtc gttggcaacg ggcgtcgtca cccccggcgc
     8941 ggccgtcaag ggtccggtgt atcgtatgct tgcaggccga ccgtcggcct gcacgagcac
     9001 ggacccgggt gccggacgta gctggaacgg cttcgccggg gcgggacgaa ttggcgtgga
     9061 ggccgttccc cgcggcggct tcggcgtcgc ctgcaggtcg tcgcggcgca cgtcgtccac
     9121 gcactcctcg tcgagctggc gctggggcag ctgatgcatc gagtcgcctg ccccgagcgc
     9181 gccgattggc aggcccgcat cggggcgctc ggcgtcgacc tcgccgcctc gccgcaatcg
     9241 ccctactggc gtgaggacgc ctactatcgg ttctccccga gtgagatcga cgcgcttcac
     9301 gatgcggcgg ccgagatcga gcggctcatc tgtgctgccg tcgatcatgt ggtnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nngacgtctc tcttcgaggc tgcggtggtg cagtggcatt ggttggaaga
     9541 ggggcatgcg gaactcgacc agtacaactc gatccacgag gcgctgatcg agacctggcg
     9601 caattggcgc cgcgatggcc ggatgtcgcg cgtgcatttc gcgtgcttcc ccgaggacga
     9661 tgacgatctg ctgaccaccg cctacctgat ggacaccgca ctccaggcgg gcctcgatgg
     9721 cgtcatgctg gaggtgccgg agattggctg ggacggccgc aacttcgtcg acctcgaggg
     9781 ccgacggatc gacgccctgt tcaagctcta cccgtgggac tggatggccg ccgatccgtt
     9841 ctttgctcac atcgcggcaa gcggcgtgcg catggtcgag ccggcctggc gcgtgatcgc
     9901 ctcgagcaag gcgctgctcg cggtgctgtg ggagcggttt cccgaccacc cgaacctgct
     9961 ggccgccagc atgcggcatt cattgatcga gcggccgcgg gtgctgaagc ccatccttgg
    10021 tcgtgagggc gccaacgtgg tgatcgagac ggctgccggg cccgtgaccc gtgcagaggg
    10081 tccctatgcc gagatggcgg cgatcgccca gacccttgcc ccgctgcccg agtacggcgg
    10141 ctggcacccc gttgtcggcg tctggatggt cggcggcgtg cctcacgggg cgggcgtgcg
    10201 ggaggatcgg agcgccatca ccggacgcgg cgcgcgcttc gtgccgcacg tgatcgcagg
    10261 gggctgaatg cgaaaggcac cattaacgcg ctggattggc tggcggatca ccggcagcta
    10321 attatccact gcaaccccga tgcgacaatc tggggcgact tcgcacttgc attttgtgca
    10381 atgcacactt aactattgca ttgcgaaggc gatgagccag cctcgtcgtc gacgcagccc
    10441 tcctttgggc gtttcctccc tagacttggg ccgttcggca acgaacggcc cctttttttg
    10501 tcgtagcgat ctcattgcca catgcagcga ccgctggcgg ctggctcaca agttgcactc
    10561 gaccgcctcg tggcccgctc ggccatccgc cacggcccac gccgcttaag ccaatgtcgc
    10621 ccacgcgcaa gcctggcgcc gatcatccgc ggccaaccat ccacagcggt cgcgatgccg
    10681 tatttgcgcc atggacgtgc cgcgatctgc caataccact cacggaccgt ggcatgaaag
    10741 tgttagtcta cagaaatgag ttgcgatgac ggcacggggc gttgtcggta gaagttaatc
    10801 agtgtaccaa tgtgagtgca tggtcggggg gccgtgcgcc gtcgcgtaca cgtgctccgg
    10861 gtcgttggag ctgatgtggc agggagtagc agccatggcg agattgctcg ggctgatgtt
    10921 gatcggctgc ggcgctggag cggcggccta tgc
//
LOCUS       DAORNH010000136        10927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4501_length_10927_cov_2.669886, whole genome shotgun sequence.
ACCESSION   DAORNH010000136 DAORNH010000000
VERSION     DAORNH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_10927_cov_2.669886"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>584)
                     /locus_tag="PK264_14015"
     CDS             complement(<1..>584)
                     /locus_tag="PK264_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD77022.1"
                     /translation="SIMNVMTTDNRTHRTVIAAVNPIVGGGGGMPTRDGTNGSGADSA
                     YLKNTPIEITEAEVPIEIVRYGLLPDSGGAGRWRGGLATSLDFKVFAPNSRITARNRD
                     RSRFRPWGTLGGKAGAASNFVVNPGTARERILGNTDFVVAEPGDIIHIHSPGGGGRGD
                     PFERESERVAADVARGYVSAAAAERDYGVVIRDGA"
     assembly_gap    585..683
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<684..1683)
                     /locus_tag="PK264_14020"
     CDS             complement(<684..1683)
                     /locus_tag="PK264_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD77023.1"
                     /translation="MSIDKVTLQVLANHTRAAAENMAYTLHRTAHSTFVKETQDFTVM
                     LLDPEGRTIAVPMDLGATWYPGISYTRAMAMVNGYEPEDIAFTNDPYSGFLATHTPDL
                     HLWKPIFHEGKIAAFTGGHIHNTDMGGAIPASLSRSLTEIHQEGIRFPPMKLYRKGKL
                     DEQALAIMMLNVRKPEQNLGDLKALVGALRTGERKVRAMIAKFGPEVFSAGLAGLLDY
                     AEHQAREILAGIPDGDYVFSDFADEDVAGGHPCRLALNLKIRGDSAILDFTGTDPQVT
                     ASLNVPTGNDPRHTLLLVGVYYVLFTLNPNLLLNQGLTRPFTCIAPEGSILNPVFPA"
     gene            complement(1692..3734)
                     /locus_tag="PK264_14025"
     CDS             complement(1692..3734)
                     /locus_tag="PK264_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD77024.1"
                     /translation="MSWRIGVDIGGTFADFCALDSASGTLHTLKVLTTPDRPGAEIGQ
                     GLDQLARQYGIAAADVAGFVHGTTVGINTVIQRRGARLGLITTAGFEDVLELARLQMP
                     DMYSLFCSRPDPLITRDCIIGVGERVRADGSIQIPLDERDVARAVSSLRSMGCAGIVV
                     SYLNGYRNSAHERRTVTLLKQLAPDLFAFSAAEVWPVVREYERTTTAILNAYVHPRVA
                     GYLDALEGELSSRGAPAAAMITKSNGGMMSARLGRRDCVGMLLSGTASGVMGAAYVAK
                     LAGVRNALTLDIGGTSADVAIIVDGEPQFGSGEKVGDFPLNIPSVSVTSIGDGGGSIA
                     SADSYGMLRVGPESAGSTPGPASYGRGGTRATLTDALVVTGYLGHAPLAYSAIKLDRA
                     AAEHAIAPLAMAIGRTPVATADAIVEIAVSNMFVEVNKLIARFGVDPRDLTLVPFGGA
                     GPMLGCLLAREIGIGQVMIPRRPGVVSALGALIADVKCDFIATVFSPVTAEAMPGLQA
                     ALAQLRQDGERWLFEQQGFKGAALVTLNADMRYVGQSFEIEVPIEERWIAEGDIEAIR
                     LAFDRRHKEIYDFADEATDVAIVNLRLVIAGPTTKPELPSVGDGSGPPPPAATIEVWS
                     EGAFHTIPLFHREALRHGHILPSPAVIAQEDTTSIIPAGFEGRIDRIGNIHLKRVN"
     gene            3923..4426
                     /locus_tag="PK264_14030"
     CDS             3923..4426
                     /locus_tag="PK264_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77025.1"
                     /translation="MAYTLEALSADIRNVLTADSGDAGKRKICEFVAKALRDKEFVEK
                     HLTAEQCRPRKVLFEDPKLGFCVCGHVYGEANTGAPHDHGSSWAIYGLATGTTVMTDW
                     KIVKQGSGDQPSLVERVRDYELKPGDAHYYPKGAVHSPMRNGPTKLIRIEGANLDHIK
                     RSNIKAA"
     gene            4441..5211
                     /locus_tag="PK264_14035"
     CDS             4441..5211
                     /locus_tag="PK264_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD77026.1"
                     /translation="MKFEYVSIERRGPVAVVGFDRKSNLNAFNEQLVDELTTAARSFH
                     DDLETHAVVLAGAAQAFSAGFDLKATESWPSESDELKRRHRAYGGVRLCKAWEEMPQI
                     TIAAMERLAVGGGVALAIACDWRVLGRSAYLYVPEVKIGLNLQWGALPRLIALVGPAR
                     AKRITILCERMGAEQALDWGLVDEVADDGETVSKALRMAEVAAAMPPATARMVKQAIN
                     ATAGALNTATSFGDADQSQLTAAYSAAAASRRSFRDKR"
     gene            complement(5208..6140)
                     /locus_tag="PK264_14040"
     CDS             complement(5208..6140)
                     /locus_tag="PK264_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD77027.1"
                     /translation="MLDRFTGMQVFVRVATLGSLSAAARALAMSQTMATKHVAALEVR
                     LGVKLLHRTTRRMTLTEAGRQYLEAAERIVADVAAADARASVQTVEVTGNLRLNTPVS
                     FGIRQIAPLLPELSRRHPGLTINLGLNDRYIDLIEEGWDLVVRIGTLAPSSLIARRLA
                     PARTVLCAAPDYLRRRGTPRTVRDLAAHDCLGYTLSRSNSAGRWNFGAGGKVGVDVTG
                     PLLANNGDALAKAAIAGLGIIYQPTFMVADEIARGELTVITLDHPPTELGGVYAVYPS
                     DRRPPAKVRAAIDYFAEVLGPVPPWERSAQPQAN"
     gene            6278..6529
                     /locus_tag="PK264_14045"
     CDS             6278..6529
                     /locus_tag="PK264_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77028.1"
                     /translation="MKTLFAALAAAAVLVGPANAKGVFETLAETAPKHPNFVELDRTA
                     PRSVLETLNETAPRTIFDGIRDTAPRSDGVFGDLERSAP"
     gene            6619..7044
                     /locus_tag="PK264_14050"
     CDS             6619..7044
                     /locus_tag="PK264_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRD77029.1"
                     /translation="MIDQRTAPYAALLLRVSLGVLLLAHGLLLKVFTFTIPGTVGYFQ
                     SIGYPGVFAYLVILGEIGGGIALILGLWTRAISLLLLPILIGATIQHLGNGWVFSAAK
                     GGWEFPAFWTVMLVVQALLGDGAWPWRPAIGAARLAEAR"
     gene            7077..7604
                     /locus_tag="PK264_14055"
     CDS             7077..7604
                     /locus_tag="PK264_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD77030.1"
                     /translation="MSNTSTLPRIEPHLNPKPIHPGTRIGHVHLKVADLDRALAFYCG
                     VLGFDLKQRYGSQAAFLAAGGYHHHLGLNTWESKGGSPPPRGTTGLFHTAILYPTRAA
                     LGDALKRLIAAGIALDGASDHGVSEALYLRDPDGNGVELYRDRDEAEWPRTASGELAM
                     VTKPLDLEALLKDAA"
     gene            7655..8209
                     /locus_tag="PK264_14060"
     CDS             7655..8209
                     /locus_tag="PK264_14060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRD77031.1"
                     /translation="MSTLKIGVVVGSLRRDSFNRKLALALAKLAPADVTFEILEIGDL
                     PLYSQDDDAAQAASVKRFKEKVKASHGILFATPEYNRSIPGVLKNAIDHASRPYGQSA
                     WAGKPAGIIGISVGAIGTALAQQHLRNITAYLDMPTLGQPEAFLQVKDGLFEADGSIG
                     AGSRQFLQAWVDRHVAWVRRHATT"
     gene            8436..9083
                     /locus_tag="PK264_14065"
     CDS             8436..9083
                     /locus_tag="PK264_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77032.1"
                     /translation="MDAGTIEDLRPLPAADVGGGTPMIKAILKNVLAVLVVGGHWYAI
                     QTGYFSPDRPTRDQWERLEQVKKLPAGKLVYQSNFATETPGGGRSEVGSWGYYNGATV
                     STVTYDPAGVTVKYGGTPWIGAKFDLAQFEAQKIYRVTIDRLVEGEPAAVIIRNRLMD
                     LERAQIPVGSGPFKVEFVAPRGGRDQIVIALIPDNRSKPQGTLRVTSLKIERLED"
     gene            9087..>10927
                     /locus_tag="PK264_14070"
     CDS             9087..>10927
                     /locus_tag="PK264_14070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRD77033.1"
                     /translation="MAARHMDMTTATAAGEKERPFALFGLMSLEAIGAAVLVGLGILY
                     IVLDNDSFFNFWTDRDMVRSEMLLRDFQWMGAELSYGSAARVPGGALHYLWVLPTLVS
                     KDPDLSYKFCVALGMLSLIPFYLAMRRSFGGVAAITATIVLMASPVLFGTLTRLWNPS
                     FQAPFIILSFAFMVRLLGERHTPSFKWLIASLVLGMQMHLSTYLLVVCVALALMVTRT
                     RIPLREVVASILLTLLLLAPYILGEIATGWDNIRQMLGSQGKGAVRSFSLTRGLLYNP
                     DNVGDVWRWYMLAFGMNDNLPSVPLRTGLSIALNLPILTGGVYTLFSIAHWLEWGKPI
                     TRTIGLPCGGAHGRVLVGAAIPVLVGFLYFSYSPQVELVIYGSSRYLMFAMPGLAIIA
                     GLGVGALLAMARGSMIARAALLVPAIVAIAMPAWALADSLRALDKPWDKPGREFMGGL
                     NQVSNEMRWDLSTLVARTAILRRHDAGRDRWRFESIFGIGYELHRTGTPVPFSQTGQC
                     AAFLTGGARVYGDKGMSPEALARSFEQPLLKLSIVRQKRFANDLLVVYERDGGQGYCF
                     TAITNRYVFSAEERAMFDRYGKIAVGAAEPVAATIPAGSKGFMTNLG"
BASE COUNT         1789 a         3591 c         3580 g         1868 t
ORIGIN      
        1 gcgccgtcgc ggatgacgac gccgtagtcg cgctcggcgg cggcggctga gacgtagccg
       61 cgtgccacgt ccgcggcaac gcgctcgctc tcgcgctcga aagggtcgcc gcgaccgcca
      121 ccgcccggcg agtggatgtg gatgatgtcg cccggctccg cgaccacgaa gtcggtgttg
      181 ccgaggatgc gctcgcgcgc tgtgcccgga ttgacgacga agttagaagc cgcgcccgcc
      241 ttgccgccga gcgtgcccca gggccgaaag cgcgagcggt cgcggttccg cgccgtgatg
      301 cgcgaattgg gcgcgaacac cttgaagtcg agcgaggtcg cgagcccgcc gcgccagcga
      361 cctgccccgc ccgaatcggg gaggagccca tagcgcacga tctcgatcgg cacctcggcc
      421 tcggtgatct cgatcggcgt gttcttgaga taggcgctgt cggcgcctga gccgttggtg
      481 ccgtcgcgcg tcggcatgcc gccgcctccg ccaacaatcg ggttcaccgc tgcgatcacc
      541 gtgcgatgcg tgcggttgtc ggtcgtcatc acgttcatga tcgannnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnncagccgg gaacacgggg ttcaggatcg agccttcggg
      721 cgcaatgcag gtgaagggac gcgtcaggcc ctggttgagg aggaggttcg ggttcaaagt
      781 gaagagcacg tagtagacgc cgacgagcag cagcgtgtgg cgcgggtcgt tcccggtcgg
      841 cacgttgagc gaggccgtca cctgcgggtc ggtgccggtg aagtcgagaa tcgcgctgtc
      901 gccccggatc ttcaggttga gcgcgagccg gcagggatgg ccaccggcca cgtcctcgtc
      961 cgcgaaatcg gagaagacgt aatcgccgtc cgggatgccg gcgagtatct cgcgcgcctg
     1021 gtgctcggca tagtcgagga ggcctgctag gcccgccgag aatacctcgg gcccgaactt
     1081 ggcgatcatg gcgcgcacct tgcgctcgcc cgtacgcagc gcgccgacaa gcgccttcag
     1141 gtcgccgagg ttctgctcgg gcttcctcac gttgagcatc atgatcgcca gcgcctgctc
     1201 gtcgagcttg cccttgcgat agagcttcat tggcggaaag cggatgcctt cctgatggat
     1261 ctccgtgagc gagcgcgaga gggacgccgg gatcgcgccg cccatgtcgg tattgtggat
     1321 gtgaccgccg gtgaaggctg caattttccc ctcgtggaag atcggcttcc acagatggag
     1381 atcgggcgta tgcgtcgcga gaaagccgct gtaggggtcg ttcgtgaagg cgatgtcctc
     1441 gggctcgtag ccattgacca tcgccattgc acgcgtgtag ctgatgccgg gataccaggt
     1501 ggcgccgagg tccatcggca cggcgatggt gcggccttcg gggtcgagca gcatcaccgt
     1561 gaagtcctgg gtctccttca cgaatgtcga gtgcgccgtg cggtgcagcg tgtaggccat
     1621 gttctcggcg gccgcgcgcg tgtgattggc gagaacctgc agcgtcacct tgtcgatgct
     1681 catggtgggc ctcaattaac gcgcttcagg tggatgttgc cgatccggtc gatgcgacct
     1741 tcgaagccgg cggggatgat cgaggtcgtg tcctcctgcg cgatcacggc cgggctcggc
     1801 aggatgtggc cgtgacggag cgcctcacga tggaatagcg gaatggtgtg gaaagcgccc
     1861 tcggaccaga cctctatcgt tgcggcggga ggcggtggac cggaaccgtc gccgacggag
     1921 ggcagctccg gcttcgtggt cggtccggcg atcacgagcc tcaggttgac gatcgcgacg
     1981 tcggtcgcct cgtcggcgaa atcgtagatc tccttgtggc ggcggtcgaa ggccagccgg
     2041 atcgcctcga tatcaccctc agcgatccag cgctcctcga tcggcacctc gatctcgaag
     2101 gactggccga cgtagcgcat gtcggcgttg agtgtcacca atgccgcgcc cttgaagccc
     2161 tgctgctcga acagccagcg ctcgccgtcc tggcgcagct gcgcgagagc ggcctggagg
     2221 ccgggcatgg cctcggcggt caccggcgag aatacggtcg cgatgaagtc gcacttcacg
     2281 tcggcgatga gggcgccgag cgcgcttacg acgccggggc gcctcgggat catcacctgg
     2341 ccgatgccga tctcgcgggc aaggagacaa ccaagcatcg ggccggcgcc gccgaacggc
     2401 acgagggtga ggtcgcgcgg gtcgacgccg aagcgagcga tcagcttgtt cacctcgacg
     2461 aacatgttcg agacggcgat ctcgacgatg gcatcggcgg ttgctaccgg cgtgcgaccg
     2521 atggccattg cgagcggcgc gatggcgtgc tcggcggcag ccctgtcgag cttgatcgcg
     2581 ctgtaggcga gcggcgcgtg gccgaggtaa cctgtgacga ccagcgcgtc agtgagcgtc
     2641 gcccgcgtgc cgccgcgccc gtatgaggcg gggcccggcg tcgagcccgc gctctcggga
     2701 ccgaccctga gcatgccgta gctgtcggcc gaggcgatcg agccgccgcc gtcgccgatc
     2761 gaggtcacgg agacgctcgg gatgttgagc gggaaatcgc cgaccttttc gccggagccg
     2821 aactgtggct cgccgtcgac gatgatcgcg acgtccgcgc tcgtcccacc gatgtcgagg
     2881 gtgagggcgt tgcgcacgcc tgcaagcttc gccacgtagg cggctcccat cacgccggac
     2941 gccgtccctg agagcagcat gccgacgcag tcccgacggc cgaggcgggc gctcatcatg
     3001 ccgccgttcg atttggtgat catggctgcc gcgggagcgc cccgcgagga gagctcgcct
     3061 tcgagggcat caaggtagcc ggcaacgcgc gggtgcacgt aggcattgag gattgcggtc
     3121 gtcgtccgct cgtattcgcg caccacaggc cagacttcgg cggcggagaa tgcgaaaaga
     3181 tcgggtgcca attgcttgag cagcgtgacg gtacgccgct cgtgcgcgct gttccgataa
     3241 ccgttgaggt aggacaccac gatcccggcg cagcccattg aacgaaggct cgacaccgcg
     3301 cgcgcgacgt ccctttcgtc tagcggtatc tgtatcgagc cgtcggcccg cacgcgctcg
     3361 ccgacaccga tgatgcagtc gcgcgtgatg agcgggtcgg ggcggctgca gaacaggctg
     3421 tacatgtccg gcatctgcag gcgggcgagt tcaaggacgt cctcgaagcc ggccgtggtg
     3481 atgaggccga gccgcgcgcc gcgccgctgg atcaccgtat tgattccgac ggtcgtgccg
     3541 tgcacgaagc cggcgacgtc ggcggcggca atgccatact ggcgggcgag ctggtcgagg
     3601 ccctggccga tctcggcgcc gggccgatcc ggcgtcgtca ggaccttcaa ggtgtggagc
     3661 gtgccgcttg cgctgtcgag cgcgcagaag tcggcaaatg tgccgccgat atcgacgccg
     3721 atgcgccagc tcatcgcgcg cctcccaggc tcatgccgtt gatccccgag gcttgcctag
     3781 catgctcccc ggacggaagg cattcgccac gtgcattcga gcccagcagc aagggctgat
     3841 ctcgattgac gcaaggggtg ttgccggcaa cgttcgcatg cataatgctg ccacatcagg
     3901 gagagccagc cggaggaacg ccatggccta cacgctcgaa gcgctcagcg ccgatatccg
     3961 caacgtgctc acggccgaca gtggtgacgc cggcaagcgc aagatctgcg agttcgtcgc
     4021 caaggcgctg cgcgacaagg agttcgtcga gaagcacctg actgctgagc agtgccggcc
     4081 gcgcaaggtg ctcttcgagg acccgaagct cggcttctgc gtctgcggcc atgtctacgg
     4141 cgaggccaac acgggcgccc cccacgatca cggctcgtcc tgggccatct acggcctcgc
     4201 caccggtacg accgtgatga cagactggaa gatcgtcaag cagggctcgg gcgatcagcc
     4261 ttcgctggtc gagcgggtgc gcgactacga gctgaagccc ggcgatgcgc actactatcc
     4321 gaagggcgca gtgcattcgc cgatgcgcaa cgggccgacc aagctcatcc gcatcgaggg
     4381 cgccaacctc gaccacatca agcgctcgaa catcaaggct gcataggcgg aacacggcgg
     4441 atgaagttcg agtacgtttc tatcgagcgc cgtggccccg ttgctgtcgt agggttcgat
     4501 cgcaagtcca atctcaacgc cttcaacgag caactcgtcg acgagctcac cacggcagcg
     4561 cggagctttc atgacgatct cgaaacccac gccgtcgtac tcgctggcgc ggcccaagcc
     4621 ttttcggccg gctttgatct caaggcgaca gagagctggc cgagcgagag cgatgagttg
     4681 aagcgccgcc accgcgccta tggcggcgtg aggctgtgca aagcatggga agagatgccg
     4741 cagatcacca ttgccgccat ggaacgtctg gctgtcggtg gcggcgtggc acttgccatc
     4801 gcctgtgact ggcgtgtact cggtcgcagc gcctacctct atgttccaga agtgaaaatt
     4861 ggactgaacc tgcaatgggg agctttgccg cggctgattg ccttggtcgg cccggcccgc
     4921 gccaagcgga tcaccatctt gtgcgagcgg atgggggccg agcaggcgct cgattggggg
     4981 cttgtcgacg aggtcgcgga tgatggcgag accgtttcca aggccctccg aatggcagag
     5041 gtggccgccg ccatgccgcc tgccacggcg agaatggtca aacaggccat caacgccaca
     5101 gccggtgcac tcaataccgc gacctcgttt ggcgatgcgg atcagagcca gttgacggct
     5161 gcctattctg ccgcagccgc ctcccgccgt tcgttccggg acaaacgcta gttggcttgc
     5221 ggctgagcgc tccgctccca gggcggcacc gggccaagca cttcggcaaa gtagtcgatc
     5281 gccgcccgca ctttggccgg cgggcggcga tcggagggat agaccgcata gacgccgccg
     5341 agctccgtcg gcggatggtc gagcgtgatc acggtgagtt cgccgcgcgc gatctcgtcg
     5401 gccaccatga aggtcggctg gtagatgatg ccgaggccgg caatcgcggc cttcgcgagt
     5461 gcatcgccat tgttggcgag caggggtccc gtcacgtcga cgccgacctt gccgcctgct
     5521 ccgaaattcc agcggccggc actgttggac cgcgaaagtg tgtagccgag gcagtcatgc
     5581 gcggcgaggt cgcggactgt gcgcggcgta ccccgacggc ggagatagtc aggcgcggcg
     5641 catagaaccg tgcgggccgg cgccagccgc cgcgcgatga ggctcgacgg cgccagcgta
     5701 ccgatcctca cgaccaggtc ccagccctcc tcgatcaggt cgatgtagcg atcgttgagg
     5761 ccgaggttga tggtgaggcc cgggtggcgc cgcgacagct cgggcagcag cggcgcgatc
     5821 tggcggatgc cgaacgagac gggcgtgttg agcctgaggt tccccgtaac ctcgaccgtc
     5881 tgaacggagg cgcgggcgtc cgccgccgcg acgtcggcaa cgatccgctc cgccgcctcg
     5941 agatactgac ggcccgcctc ggtgagcgtc atccggcgcg tcgtgcgatg caggagcttt
     6001 acgccgagcc gcacttcgag tgcggccacg tgtttcgtcg ccatggtttg cgacatggcg
     6061 agcgcgcgcg ctgccgccga aaggctgccg agcgttgcaa cccgaacgaa cacctgcatt
     6121 ccggtgaagc ggtccagcat attctgtctc acacctatgg tgtgatataa tatccagttt
     6181 ctgctaatta tcaaactatt ggatcgaggg catcttcggc tcatcaacac ggtccgaccc
     6241 gaacggtggt tcgggccgca aacaaggaga tgatcccatg aagaccctgt tcgccgctct
     6301 cgccgccgcc gccgtcctcg tcggccccgc caatgccaag ggcgtgttcg agacgctcgc
     6361 cgagacggcg ccgaagcatc ccaacttcgt cgagctcgac cgcacggccc cgcgctcggt
     6421 gttggagacg ctcaacgaga ccgcgccgcg caccatcttc gatggcatcc gcgacacagc
     6481 tccccgctcc gacggcgtgt tcggcgacct cgagcgctcg gcgccctgac ctcgagcgtc
     6541 gcgctttcgc tgtgcccctg cggcacggcg accgccaccc tggacttcga cccgctaccc
     6601 accgcaaagg cttccaccat gatcgaccaa cgcaccgcgc cctatgccgc cctactcctc
     6661 cgcgtgagcc tcggcgtgct ccttcttgcg cacggccttc ttctgaaggt cttcacgttc
     6721 accatccccg gcaccgtcgg ctacttccag agcatcggct atccgggcgt cttcgcctac
     6781 ctcgtgatcc tcggcgagat cggtggcggc atcgccctga tcctcggtct gtggacacgc
     6841 gccatctccc tcctgctgct gccgatcctg atcggcgcga ccatccagca tctcggcaac
     6901 ggctgggtgt tttcggcggc gaagggcggt tgggagtttc cggccttctg gacagtcatg
     6961 ctagtcgtgc aggcactgct cggtgatggc gcctggccct ggcggcccgc gatcggcgct
     7021 gcccgccttg ccgaagcccg ctgaagtaac gccgcgctca tcgaggtaaa atcgccatgt
     7081 cgaacaccag cacgctgccg cggatcgagc cacatctcaa tccgaagccc atccatccgg
     7141 gcacccggat cgggcacgtc cacctgaaag tcgccgacct cgaccgcgct cttgccttct
     7201 actgcggcgt cctcggcttc gacctgaagc agcgctatgg cagccaagcc gctttcctcg
     7261 cggcgggtgg ctaccaccat cacctcgggc tcaatacctg ggagagcaag ggcggctcgc
     7321 cgccgccgcg gggaacgacc gggctattcc acaccgcgat cctctacccg accagggcgg
     7381 cactcggcga tgccctgaag cggctcatcg ccgccggcat cgccctcgat ggcgcgagcg
     7441 accacggcgt cagcgaggcg ctctacctcc gcgatccgga tggcaatggg gtcgagctct
     7501 atcgagaccg agacgaggcc gagtggccgc ggacggcgag cggcgagctt gccatggtga
     7561 cgaagccgct cgacctcgag gccctcttga aggatgccgc gtagacccgc ggcaacctta
     7621 cccacgcaat cgaacacgta gtcaggatca catcatgagc acattgaaaa tcggtgtcgt
     7681 cgtcggcagc ctccgccgcg acagcttcaa ccgcaagctt gctctggcgc tcgccaagct
     7741 ggcgccggcc gacgtcacct tcgaaatcct cgagatcggc gatctgccgc tctactcgca
     7801 ggacgacgac gcggcgcaag cggcctccgt caagcgcttc aaggagaagg tcaaggcctc
     7861 gcacggcatc ctgttcgcga cgcccgagta caaccgctcg atcccgggag ttctcaagaa
     7921 cgcgatcgat catgcctctc ggccctatgg ccagagcgcc tgggccggca agcccgcggg
     7981 catcattggc atctcggtcg gcgccatcgg caccgcgctt gcccagcagc atctgcgcaa
     8041 catcacggcc tacctcgaca tgccgaccct cggccagccc gaggcgttcc tccaggtcaa
     8101 ggacggcctc ttcgaggccg acggcagcat cggcgcggga agccgccagt tcctccaggc
     8161 gtgggtcgat cgccacgtcg cctgggtgcg ccggcacgcg acaacctgac ctccacaccc
     8221 cggacttgcg cggcgccgag gcccgcgctc gttgccggcg aagcacagtg gcatcattca
     8281 aggggagcgg ccgggttgcc gctccctttc aatttgcggg gactgccgtc aggatggacc
     8341 ggcgcgctcc aagtcagtgg aactacggat tgcacgcgcg tatgcctcta gccgaagtct
     8401 cgggcaaatt tggggggacg agtgggcagt cgaccatgga cgcgggaacc atcgaggatt
     8461 tgcggccgct gcccgctgcg gacgtcgggg gcggcacgcc gatgatcaag gccatcctga
     8521 agaatgtcct ggcggtactg gtggttggcg gccactggta cgcgatccag accggctatt
     8581 tttcgccgga tcgaccgacg cgcgaccagt gggagcgcct cgagcaggtg aagaagctgc
     8641 cggcgggcaa gctcgtctac cagtccaact tcgcgaccga gacgcctgga ggcggccgca
     8701 gcgaggtcgg cagctggggc tactacaacg gcgcgaccgt atcgacggtc acctacgatc
     8761 cggccggtgt caccgtgaag tacggcggca cgccctggat cggcgccaag ttcgacctgg
     8821 cccagttcga agcgcagaag atctaccgcg tcaccatcga tcgcctggtc gagggcgagc
     8881 ctgcagcggt catcatccgc aatcggttga tggacctcga gcgtgcccag atcccggtcg
     8941 gcagcggtcc cttcaaggtc gagttcgtgg cgccaagagg cggccgcgac cagatcgtca
     9001 tcgccctgat cccagacaat cggtcgaaac cgcagggcac acttcgcgtg acgtcgttga
     9061 agatcgagcg gctggaggat tgagccatgg ccgcacgtca catggatatg acgacagcga
     9121 cggccgcggg cgagaaagag cgcccgttcg cactcttcgg cctcatgtcg ctcgaggcga
     9181 tcggtgccgc ggtgctggtc ggcctcggca tcctctacat cgtgctcgac aacgactcct
     9241 tcttcaactt ctggactgac cgcgacatgg tccgctcgga gatgctgctc cgtgacttcc
     9301 agtggatggg agcggagctc tcctacggtt cggcggcgcg tgtccccggc ggcgcactgc
     9361 actatctctg ggtgctcccg acgctcgtca gcaaggatcc cgacctcagc tacaagttct
     9421 gcgtggcgct cggcatgctg tcgctgatcc ctttctatct cgccatgcgc cggagcttcg
     9481 gcggggtcgc ggcgatcacg gcgacgatcg tgctgatggc ctcgcccgtg ctcttcggca
     9541 cgctgacgcg gctgtggaac ccgagcttcc aggcgccgtt catcatcctg agcttcgcct
     9601 tcatggtgcg gctgctcggc gaacggcaca cgccgagctt caagtggctc atcgcttcgc
     9661 tcgtactcgg catgcagatg cacctgtcga cctatctcct ggtcgtctgc gtagcgctcg
     9721 ccctcatggt cacgcgcacg cgcatccctc tgcgtgaggt agtcgcttcg atactgctga
     9781 cactcctgct gctggctcct tacattctcg gcgagatcgc cacgggctgg gacaacatcc
     9841 gccagatgct cggcagccag ggcaagggcg ccgtgcgctc ctttagcctc acgcgcggcc
     9901 ttctctacaa tcccgacaac gtcggcgacg tgtggcgctg gtacatgctg gccttcggca
     9961 tgaatgacaa cttgccgtcc gtgccgctgc gcaccgggct gtcgattgca ttgaatctcc
    10021 ccatcctgac cggcggcgtc tacacgctgt tttcgatcgc gcactggctc gaatggggaa
    10081 agccgatcac ccgcaccatc ggattgccgt gcggcggcgc ccacggccgg gtgctcgtcg
    10141 gtgcggcgat ccctgtgctg gtcggttttc tatacttcag ctattcgccg caggtcgagc
    10201 tcgtgatcta cggctcgtcc cgctacctca tgttcgcgat gccgggcctc gccatcatcg
    10261 ccgggctcgg cgtcggcgcg ctgctcgcca tggcgcgcgg ctcgatgatc gcgcgggcgg
    10321 cgctgctcgt cccggcaatc gtcgccatcg ccatgccggc ctgggcgctc gccgattcac
    10381 tgcgcgcgct cgacaagcct tgggacaagc ctggccgcga attcatgggc ggcctcaacc
    10441 aggtcagcaa cgagatgcgc tgggacctgt cgacgctcgt cgccaggacc gcgatcctgc
    10501 gacgccatga tgctggcaga gatcgctggc ggttcgaatc gatcttcggc atcggctacg
    10561 aattgcaccg tacgggcacg cccgtcccct tctcgcagac gggccagtgt gcggccttcc
    10621 tgacgggcgg cgcgcgcgtc tacggcgaca agggcatgag ccctgaggcg ctcgcccgct
    10681 cgttcgagca gccgctgctg aagctctcga tcgttcgcca gaagcgcttc gcgaacgacc
    10741 tcctcgtcgt ctacgagcgc gacggcggac agggctactg cttcactgcc atcacgaacc
    10801 gatacgtctt ctcggccgag gagcgggcga tgttcgatcg ctacgggaag atcgcggtcg
    10861 gggccgccga acccgttgcg gcgacgatcc cggccggcag caaaggtttc atgacgaacc
    10921 tcgggag
//
LOCUS       DAORNH010000137        10878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4526_length_10878_cov_2.551418, whole genome shotgun sequence.
ACCESSION   DAORNH010000137 DAORNH010000000
VERSION     DAORNH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4526_length_10878_cov_2.551418"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..978)
                     /locus_tag="PK264_14075"
     CDS             complement(37..978)
                     /locus_tag="PK264_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77034.1"
                     /translation="MSRRKRLLSIAGLLALVATPGPARGEDMTGTWIAVHGLMGDVIG
                     HIEELTVADDGAAVVNIYKVPIDQPCIAPGRLGRPECISAADYVGGRIAVDSSARKLI
                     LKGALSGKAALPGIDGVLGQIYWLAPDIEYFLFRKDRILDISAAPANAPDGAPHPALR
                     LPPTKRFVRAEPGLAAALIIYAQLAEEPVIDLACVLPLAAETEDGFAKFRALVIDGER
                     LGRFLDATEARAEAGESIAAKGAAREQLREITGLASDDPGYAAKRLRQAALIGISRAN
                     LELYLRPKAANLPAGLPLPPGLPKLRDIAECRSSLRR"
     gene            1369..1791
                     /gene="folB"
                     /locus_tag="PK264_14080"
     CDS             1369..1791
                     /gene="folB"
                     /locus_tag="PK264_14080"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRD77035.1"
                     /translation="MQHDRELELRRGVSDTRGSAASGRRRVFIRDLELVASVGVFEVE
                     RRYQQRIVVSVSLDVIDSYDGVSDRLDDVLDYAEIVRRITRIADADHTNLIETLAERF
                     AAACLADTRVRAVTVRIEKPDIMPSCKSVGVEITRTSG"
     gene            complement(1861..3060)
                     /gene="sucC"
                     /locus_tag="PK264_14085"
     CDS             complement(1861..3060)
                     /gene="sucC"
                     /locus_tag="PK264_14085"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRD77036.1"
                     /translation="MNIHEYQAKELYRQYGVPTATGYAAMSIDEAVAGARKLPGPVWV
                     VKAQIHAGGRGKGKFKEAAAGDKGGVRLAKSIDEVKAFAGQMLGNTLVTAQTGAAGRK
                     VQRLYVEDGSQIARELYLSALVDRNTSRIAFIVSTEGGMDIEKVAHDTPEKIHTFTID
                     PASGYSGHHGRKIALALGLSGDLMNQCVAMVGGLYRMFVEKDMSLLEINPLVVTKDGK
                     LLALDGKMNFDSNALFRHKEIVALRDLAEEDAAEVEASKYDLNYVKLDGQIGCMVNGA
                     GLAMATMDIIKLYGLEPANFLDVGGGATKEKVTAAFKIILSDPNVKGILVNIFGGIMR
                     CDIIAEGVVAAAKEMAVKVPLVVRLEGTNVELGKQILAQSGLKIVPADNLADAAKKIT
                     DEVKKAT"
     gene            3233..4960
                     /locus_tag="PK264_14090"
     CDS             3233..4960
                     /locus_tag="PK264_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77037.1"
                     /translation="MSMLTVRERVSGRLMWQARRCAGFSIAGLIGALAIVGAARAETP
                     ACTLADLAAGVATCSCPDGARREGPVCAAVPDPRFKGPRRHVLLRVARLQFCARELGH
                     YEGPVTGKMTTPTLRALGKIRVAAKLRYPRDASKDGPLHAEVWRLCRVVWASRIALAK
                     KSAPAAPLVTPRIDPPVQPAPQIGIPAPQPTMRPKDGLPGAAICLPKELAELIAGNAS
                     GRAAVPVCELPCIARPVSLSGEDARALERRHGVRWCGTCVTIATPLPVEEALRIESAG
                     NITLCPRSLPRPIEILSSGRIVQDALQGVRAAFGRADQRPATRHDHVALVVTVARSAL
                     SGGGKPIAERDGAAMKALLLERLGYRGDQVLHLANPTRADLYRALAPGAENPIGRRLG
                     AVGGANLFVYVSARGLSEDEGESPLILLADAVAGRETQGAIAVDAFVQALARLTPSGL
                     TVVLEVPFLGRAAGALVPPNASMTGTSALPPPVRGLVVMTSSERDQHALEDVQLRLSL
                     FTRHLVEAMAGAADAAPIGNGDRVIDTAEAFVLASFRTQLAARKTWGLLQRPVLSRGP
                     ALPLVHLPP"
     gene            complement(4970..>5806)
                     /locus_tag="PK264_14095"
     CDS             complement(4970..>5806)
                     /locus_tag="PK264_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRD77038.1"
                     /translation="GQARDAGKSSALEARYGREATPEAGPWNSVLDSLLAHRTVRGYK
                     PDALPSGTLETLIAAAQSAATSSNLQTWSVVAVTDPAKKGTFAKIAGGQKHIEQCPLF
                     LAFLADLSRLGRLSDAEGRNLELAGLPYLETFLVAAIDAALAAQNAVVAAESLGLATV
                     YIGAMRNDPVRVAEELGLPPGAACVFGLCIGYALPTHTGEVKPRLAQPVVLHRERYAS
                     SEDAANRAAYDRLLEDFSRRNEMSAYTWTERVLNRIGKVTALSGRDRLKEQLRRLGFP
                     LR"
     assembly_gap    5807..5816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5854..>6384)
                     /locus_tag="PK264_14100"
     CDS             complement(5854..>6384)
                     /locus_tag="PK264_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77039.1"
                     /translation="AILSAITPPVALAVFAAAGLAKADLWKSGWAAMKIGAAGFVVPF
                     MFVYEPALLMFDPLVKSKDLITANWLDIARATATASLGIMLLAAGLHGFIWGRLDLWQ
                     RALACAGAACLILPTLVTDIAGVSAAAALFVSQKMLGRSGKDRPAAPPPPAEDEVATS
                     SGGAPVASAAARGPLT"
     assembly_gap    6385..6482
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6483..7974)
                     /locus_tag="PK264_14105"
     CDS             complement(<6483..7974)
                     /locus_tag="PK264_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRD77040.1"
                     /translation="MSIDPGHRGIVRLVAGLIAIAMSAFHMWVVAFGAPEPVIFRATH
                     LLFAMLLVFLWFRMSGDSTEQPSLLDYALLIAGAIAVGYQLYSYEYIIGRMYYVDDLK
                     PIDWVLAIVLIATLLEATRRTIGPALPITALVFIAYALLVARVEPQRFLDQAYLTTEG
                     IFGIPLSVSASYVMIFVLFGSFMERTGTGQLFMDFALSLTGHTAGGPGKVSVVSSSLF
                     GTISGSAVANVMVDGPISIPLMKRTGFKAHFAAAVEAVASTGGQIMPPIMGAAAFVMA
                     EFLRVGYLQVVVWALIPSILYYVACFYAVHFEAKRQGIVGLPRSELPRLGQTLKERGH
                     LFIPVLTILAIMYSGYSAPMAALGGTLLCFPAAWFGPIVLLLLPLLILAPHLAPKIFP
                     ALVPTFTASGFIEAAIITALVLLWTYRTWGRIVLLPIQGWPIIESMIDAAKNGLGVAM
                     ACACAGIIIGIISLTGVGLTFTQFVVGLAQNTLLLALVLTMVAGIIL"
     gene            complement(8083..9027)
                     /locus_tag="PK264_14110"
     CDS             complement(8083..9027)
                     /locus_tag="PK264_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRD77041.1"
                     /translation="MHKALIRASIGAAALFAVTGTVAAQELRFMTGPQGGIWVPLGGA
                     LKDLWEKNIPGTKVAALPGAGVVNVRGIEENKADIGFGNTITTVDALAGNAPFPKPHT
                     SVCNLATLYPQYFQVVVLANSGMSKVTDFKGKTLTTLPRGNTTEAANMHMLKANGITY
                     SDLKVTFGSITDSVTQMQDGQATVWAVATGIPAAGIMDLAAGREVRLIDLGGTVDAMK
                     KINPGYSLGVIPAGTYPKQTTEIKVPTFGAHLVINCKQPEDLVYKMTKAIAENVPQIA
                     LVHKGMAKLTVAEMGTDIGVPMHKGALKYYTEKGIKTK"
     gene            9248..10051
                     /locus_tag="PK264_14115"
     CDS             9248..10051
                     /locus_tag="PK264_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77042.1"
                     /translation="MASFAFADNLILIFKVTVTPLLVALASLAARRWGPRVGGILIGF
                     PVMTGPIALFLALEQGVAFAARASVGILLALIAVAGWIVIYAWLAPRARWPLALALAS
                     VGYVLPGYWLAGLETRPAVAAALAIAAILAAIVAIGHPRGARITGPVPWWDIWLRMLV
                     SATLVTGITLAAERLGPMASGIVGTFPAISTVVVTFTHHRWGAGAATAIMRGLLLSLL
                     SFASCFLVIGELIISHGIAVSFLLGVLAGGVTSLAVLSADGWLRRSSES"
     gene            10158..>10467
                     /locus_tag="PK264_14120"
     CDS             10158..>10467
                     /locus_tag="PK264_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRD77043.1"
                     /translation="MMKSKALLAVAVIAGAAIGASALVNAQDVIAQRKALMKATGGAF
                     KTATDMAKGEAPFDAAKASAAMKKIADDWPVFMKLFPDNSKTGGETTAHGDIWTKRAE
                     F"
     assembly_gap    10468..10477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1779 a         3375 c         3716 g         1890 t
ORIGIN      
        1 aggccggcgg gcgatggctg cggcggattc ggcacgtcat cgcctcagcg agctgcggca
       61 ttcggcaatg tcgcggagct ttggcagccc cggtggcagc ggcaggccag ccggcaggtt
      121 cgcagccttt ggccgcaagt agagctctag attggccctg ctgatgccga tcagcgcggc
      181 ctgcctcagg cgcttggcag cgtagccagg gtcgtcactt gcgagaccgg tgatttcgcg
      241 aagctgttcg cgcgccgcgc ctttggccgc gatcgactcg cccgcttctg cgcgtgcttc
      301 cgtcgcgtcc agaaagcggc caagacgctc tccatcaatc accagggcgc ggaatttcgc
      361 aaatccgtcc tcggtttcgg ctgcgagcgg caggacacag gctagatcga tcaccggctc
      421 ttcggcaagt tgggcataga tgatgagcgc ggccgcaaga ccgggctcgg cacgcacgaa
      481 gcgtttcgtc ggggggaggc ggagggccgg atgaggcgcg ccatcgggag cgttcgccgg
      541 tgcggcgctg atgtcgagga ttcggtcctt ccggaacagg aaatactcga tgtctggcgc
      601 aagccaatag atctgcccaa ggacaccgtc gatacccggc agtgcggcct tccccgagag
      661 ggcgcctttc aggatcaatt tcctggcgga actgtcgacc gcgatgcggc cgccgacata
      721 atcagcggcc gaaatgcact cgggcctgcc gagccgccct ggtgcaatgc agggttgatc
      781 gatgggaacc ttgtatatgt tgacgacagc ggcgccgtcg tctgcgacgg tcaactcctc
      841 gatatgcccg atcacgtcgc ccatgagccc atgcaccgcg atccaggtgc cggtcatgtc
      901 ctcgccgcga gccgggcctg gggttgcgac aagggccaga agaccggcga tcgacagcaa
      961 ccgcttgcgc ctggacatga tgacctcgat gaatacggcc gcctgcatgc acaacatttg
     1021 gagccgatgc aggagtttat tcgcggcgcc gccaatggtg tagcaggccc gcggtttcat
     1081 tgcccgcggt ttcatgcccg aggcgtgatg cgggcgcccc gaccttcgag acgggaagcc
     1141 ctcggcctca ccggccgcct tagtcttcgt cgtcgaggct atcgccgaac gtcgagcggc
     1201 tcgcacatca atttcaatcg cctcgccaaa gatggcatag aggtcgtccg cttcctgcac
     1261 gggcgcatgg acgctcacgc cacatttgac ctgccctcag tccaatactg cattgattgc
     1321 gccagggtat gaagtagatc agtctatgac tgacaggagc gcgtccgaat gcaacatgac
     1381 agagaactcg agttaagaag gggggtaagc gatacgcgcg gctctgcagc ctccggtcgg
     1441 cgccgcgtct tcatccgcga tctcgagctt gtcgcgagtg tcggcgtttt cgaggtggag
     1501 cggcgctacc agcagcggat cgtcgtctcg gtttcgctcg acgtgatcga ttcctacgac
     1561 ggcgtgtccg accggctcga cgacgtgctc gactatgccg aaatcgtgcg ccggatcacc
     1621 cgcatcgccg atgccgacca caccaacctc atcgagacgc tggccgagcg ctttgccgct
     1681 gcttgcctcg ccgacaccag agtgagagcc gtcaccgtca ggatcgaaaa gccagacatc
     1741 atgccgtcat gcaagtccgt cggtgtggag atcacgcgga cgagcggctg aggtctcgcc
     1801 ggttggcgag gcctcaggtg gcgtgcgggg caggggcccc gctgttatcg ttcggcctcc
     1861 tcacgtcgcc ttcttcacct cgtcggtgat cttcttggcg gcgtcggcga ggttgtcggc
     1921 ggggacgatc ttcaggccgg actgggcgag gatctgcttg ccgagctcga cgttggtgcc
     1981 ctcgaggcgg acgacgagag gcactttcac ggccatctcc ttggccgccg ccaccacgcc
     2041 ttcggcgatg atgtcgcagc gcatgatgcc gccgaagatg ttgacgagaa tgcccttcac
     2101 gttcgggtcg gaaaggatga tcttgaacgc tgccgtcacc ttctccttgg tggcgccgcc
     2161 gccgacgtcg aggaagttgg cgggttccag gccgtagagc ttgataatgt ccatggtcgc
     2221 catggcgagg ccggcgccgt tcaccatgca gccgatctgg ccgtcgagct tcacgtagtt
     2281 gaggtcgtac ttggaggcct ccacctcggc cgcgtcctcc tcggcgaggt cgcgcagcgc
     2341 cacgatctcc ttgtgacgga agagggcgtt cgaatcgaag ttcatcttgc cgtcgagggc
     2401 gagcagcttg ccatccttgg tgacgaccag cgggttgatc tcgaggaggc tcatatcctt
     2461 ctcgacgaac atccggtaga ggccgccgac catggcgacg cactggttca tgaggtcgcc
     2521 cgagaggccg agggcgagcg cgatcttgcg gccgtggtgg cccgagtagc cggatgcggg
     2581 atcaatcgtg aacgtgtgga tcttctccgg cgtatcgtgg gcgactttct cgatgtccat
     2641 gccgccttcg gtcgagacga tgaaggcgat gcggctcgta ttgcggtcga ccagggcgga
     2701 gaggtacagt tcgcgcgcga tctggctgcc gtcctcgacg tagaggcgct gcaccttgcg
     2761 gccggcggca cccgtctggg cggtgacgag cgtattgccg agcatctggc cggcaaaagc
     2821 cttcacctcg tcgatcgact tggcgagccg gacgccgccc ttgtcgccgg cggcggcttc
     2881 cttgaacttg cccttgccgc ggccgcccgc atgaatctga gccttgacca cccagaccgg
     2941 gcccgggagc ttcctggcgc cggccaccgc ctcgtcgatc gacatggccg catagcccgt
     3001 ggccgtcggc acgccgtact ggcgatagag ctctttggcc tgatattcat ggatgttcat
     3061 gggcgcgtcc cgtgcttggc gtgcattcgg agccgtcata gccgctcggg gcggctttgc
     3121 aaagtttggc tttcgacggg ggccaaagtc aggaaagtgc ggttggcatg cgcctcgcat
     3181 ggcgcaccgg ccgcccaccg tcacggcgtg gctggcgcgt catggggcaa acatgtcgat
     3241 gttgaccgtc cgggagagag tctcgggtcg cctgatgtgg caggctcgca gatgcgcggg
     3301 cttcagtatt gccgggctga tcggggcgct tgcgattgtc ggcgcggccc gggccgagac
     3361 gccggcgtgt acgctggccg acctcgcggc cggcgtcgcg acctgctcat gcccggacgg
     3421 cgcgcggcgc gaggggcccg tctgtgccgc ggtgcccgat ccgcgcttca aaggcccgcg
     3481 ccgccatgtc ctgctgaggg tcgcccgcct ccaattctgt gcgcgagagc ttggccacta
     3541 cgaaggcccc gtcacgggga agatgacgac accgacgctg cgtgcgctcg gcaagatccg
     3601 cgtcgcagcg aagctgcgct atccgaggga tgcgtcgaag gacggtccgc tgcatgcgga
     3661 ggtgtggcgc ctgtgcaggg tcgtatgggc gagccggatc gcgctggcaa aaaagtccgc
     3721 tccggcagcg cccttggtaa cgccgcggat cgatcctcct gtgcagcccg cgccccaaat
     3781 tggaataccg gcgccgcagc caacgatgag gccaaaggat ggcctgccag gcgcggcgat
     3841 ctgcctgccg aaagagcttg ccgagctcat cgcagggaat gcctcaggcc gcgccgccgt
     3901 gcccgtgtgc gagctgcctt gcattgcccg cccggtgagc ctctcgggtg aggatgcccg
     3961 agcgctcgag cgtcggcacg gcgtgcgctg gtgtggtacg tgcgtgacca ttgccacgcc
     4021 gcttcccgtc gaggaggcct tgcgcatcga gagcgcgggc aacatcacgc tctgcccgcg
     4081 ttcgctgccg cggccgatcg agatcctgag ctcgggccgg atcgtccagg atgcgctgca
     4141 aggcgtgcgc gcggcgttcg gccgggcgga tcagcggccg gcaacgcggc atgatcatgt
     4201 ggcgctagtt gtgaccgtcg cgcgctcggc actgtcgggt ggcggaaaac caatcgccga
     4261 gcgcgacggc gccgctatga aagcgttgct gctcgagcgg ctcggctatc gcggcgacca
     4321 ggtgctgcat ctcgctaacc cgacgcgcgc cgatctctat cgcgcgctgg caccgggcgc
     4381 cgaaaacccg atcggcaggc gcctcggtgc tgtcggcggg gccaacctgt tcgtgtacgt
     4441 gtcggcgcgc ggcctatcgg aggacgaggg cgagagcccg ctcatcctgc tcgctgatgc
     4501 cgtcgccgga cgcgagaccc agggcgcaat cgctgtcgac gcgttcgtcc aggcattggc
     4561 tcggctcacc ccgagcggtc tcacggtggt gctggaggtg ccctttctcg ggcgtgccgc
     4621 gggcgcgctg gttccgccta atgcctcaat gacggggacg tcagcactgc cgccgccggt
     4681 tcgggggctc gtggtcatga catcatccga acgcgaccag catgcgctcg aagatgtgca
     4741 gctgcggctc tcgctgttca cgcggcacct tgtcgaggcc atggcgggtg cagccgacgc
     4801 agccccgatc ggcaacggcg accgggtcat tgacacagcc gaggcctttg tgctcgcttc
     4861 gtttcgcacg caactcgcgg cgcgcaagac gtggggtctc ctgcagcgac ccgtgctcag
     4921 ccggggaccg gccctgccgc tcgtgcacct gccgccgtga ggcgggcggc tatcggagag
     4981 gaaagccgag gcgccgaagc tgctctttga gccggtcgcg gccggagagc gcggtgacct
     5041 tgccgatacg gtttaggacg cgctcggtcc aggtgtaggc ggacatctcg ttgcggcgcg
     5101 agaagtcctc gagcaggcga tcgtaggcgg ctcggtttgc agcatcctcg cttgaggcgt
     5161 agcgctcgcg atgcaggacg acgggctgcg cgaggcgtgg ctttacttca cccgtatgtg
     5221 tcggcaaggc atagccgatg cacaacccga aaacgcaggc ggcaccaggt gggagtccaa
     5281 gctcctcggc aacgcgcacg gggtcgttgc gcatggcacc gatatagaca gtcgcgaggc
     5341 cgagcgattc ggcggccacg acggcgttct gggccgccag cgcggcatcg atcgccgcga
     5401 cgaggaacgt ctcgagatag gggaggccgg caagctcgag gttgcgccct tcggcgtccg
     5461 acagccggcc gagcctcgag agatcggcga ggaaagcgag gaacagcggg cactgctcga
     5521 tgtgcttctg cccgccggcg atcttcgcga acgtcccctt cttggcggga tccgtgacgg
     5581 cgacgaccga ccaggtctgc aggttcgagc tggtggcggc cgactgggcg gcggcgatca
     5641 gcgtctcgag cgtgccggag ggtagcgcgt ccggcttgta gccgcgtacg gtgcggtgcg
     5701 cgaggaggct gtcgaggacg ctgttccacg gtcctgcctc gggcgtggcc tcccggccat
     5761 aacgcgcttc gagcgcactc gatttccccg cgtcgcgggc ttgtccnnnn nnnnnntgca
     5821 tgcgcttggt cctgcgttct tgtgattgcc gcgtcaggtg agcgggcccc tcgcggcagc
     5881 tgatgccacc ggggcgccgc ccgatgatgt cgccacttcg tcctcggccg gaggtggcgg
     5941 agcggcaggc ctgtccttgc cgctgcggcc gagcattttc tgcgaaacga acaaggctgc
     6001 tgctgcgctg acgccggcga tgtcggtgac gagtgtcggg aggatgaggc aggccgcacc
     6061 ggcgcaggca agggcacgct gccacaagtc gaggcggccc cagatgaagc cgtgcaggcc
     6121 tgccgccagc aacatgatgc cgagcgaggc tgtggccgtg gccctggcga tatcgagcca
     6181 attggcggtg atgaggtcct tcgacttcac gagcggatcg aacatcagaa gcgcgggctc
     6241 atacacgaac atgaagggca cgacgaagcc ggcggcgccg attttcatgg cggcccagcc
     6301 gctcttccac agatccgcct tggcgagacc cgctgcggcg aacactgcaa gcgccaccgg
     6361 cggcgtgatc gccgacagga tcgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nncgaggatg atgccggcca ccatggtcag gacgagggcg aggagcagcg tgttctgggc
     6541 gaggccgacg acgaactgcg tgaaggtgag cccgacgccg gtcagcgata tgatgccgat
     6601 gatgataccg gcgcaggcgc aggccatggc gacgccgagg ccgttcttgg cggcgtcgat
     6661 catggattcg atgatcggcc atccctggat cggcagcagc acgatgcggc cccaggtgcg
     6721 gtaggtccag agcagcacga gagcggtgat gatggcggct tcgatgaaac cgctggcggt
     6781 aaaggtcggc acgagcgccg ggaagatctt cggcgcgagg tgcggggcga ggatcagcag
     6841 cggcagcagc aggagcacga tcgggccgaa ccaggccgcg gggaagcaga gcagcgttcc
     6901 accgagcgcg gccatgggcg cgctgtagcc ggaatacatg atggcgagga tggtgagcac
     6961 cgggatgaag agatgcccgc gctccttgag tgtctgtccg aggcgcggca gctcggatcg
     7021 cggcaggccg acgatgccct ggcgcttcgc ctcgaagtgg acggcataga agcaggcgac
     7081 atagtagagg atcgagggga tcaaggccca cacgacgacc tgcaggtagc cgacgcgcag
     7141 gaactccgcc atcacgaaag cggcagcgcc catgatcggc ggcatgatct gcccgccggt
     7201 cgaagcgaca gcctcgacgg cagccgcgaa gtgcgccttg aacccagtcc gcttcatcag
     7261 ggggatcgag atcggcccgt cgaccatcac gttggcgacg gcggagcccg agatcgtgcc
     7321 gaagagcgag gaggagacga ccgagacctt gccgggaccg ccggccgtat gcccggtcag
     7381 gctcagtgcg aagtccatga acagctgccc cgtgccggtc cgctccatga agctgccgaa
     7441 cagcacgaag atcatcacgt aggaggccga gaccgagagc gggatgccga agatgccctc
     7501 ggtggtgaga taagcctgat ccagaaagcg ctgcggctcg acgcgggcga cgagcagggc
     7561 gtaggcgatg aagacgagcg cggtgattgg cagcgccgga ccgatcgtgc gccgtgtcgc
     7621 ttcgagcagg gtggcgatca ggacgatggc cagcacccag tcgatcggct tcaggtcgtc
     7681 gacatagtac atccgcccga tgatgtactc gtaggagtag agctggtagc cgaccgcgat
     7741 ggcaccggcg atcagcaagg catagtcgag gagggagggc tgctcggtcg aatcccccga
     7801 catgcgaaac cagaggaaga cgagcagcat cgcgaacagg agatgcgtcg cgcggaagat
     7861 gaccggttca ggtgcgccga acgcgacgac ccacatgtgg aacgcggaca tcgcgatggc
     7921 gatgaggccg gcgaccaagc gcacaatgcc acggtgacca ggatcgatgg acacgtttct
     7981 tcccccgtac acaggactaa gcggacggca tcacccccgt gaagccgtcc gccaggtgaa
     8041 tttcgtgcgg gccagtgcgc gaggcaccgc gcccgcgcgc gatcacttcg tcttgatgcc
     8101 cttctcggtg tagtacttga gggcgccctt gtgcatcggc acgcctatgt cggtgcccat
     8161 ctcggcaacc gtcagcttcg ccatgccctt atgaacgagc gcgatctgcg ggacgttctc
     8221 ggcgatcgcc ttcgtcatct tgtagacgag gtcttcgggc tgcttgcagt tgatgacgag
     8281 gtgcgcgccg aaggtcggca ccttgatctc ggttgtctgc ttcggatagg tgccggcggg
     8341 aatgacgccg agagaatagc cgggattgat cttcttcatc gcgtcgaccg tgccgccgag
     8401 atcgatcaac ctaacctcgc ggcccgcggc gagatccatg atgccggccg ccggaatacc
     8461 ggtcgcaacg gcccacacgg tcgcctgtcc gtcctgcatc tgcgtaacgg aatccgtgat
     8521 cgagccgaac gttaccttga ggtcactgta cgtgatgccg tttgccttca gcatgtgcat
     8581 gttggcggct tcggtggtgt tgccgcgcgg cagcgtggtg agcgtcttgc ccttgaagtc
     8641 ggtaactttg ctcatgccgc tgttggcgag cacgacgacc tggaaatatt gcgggtagag
     8701 cgttgccagg ttgcagacgc tcgtgtgcgg cttcgggaac ggcgcattgc cggcgagcgc
     8761 atcgaccgta gtaatggtgt tgccgaagcc gatgtcggcc ttgttctcct cgatgccgcg
     8821 aacattgacc acgccggcgc cggggagagc cgcgaccttg gtgccgggaa tattcttctc
     8881 ccagaggtcc ttcagcgcgc cgccgagtgg cacccagatg ccgccttgcg gcccggtcat
     8941 gaaccgcaat tcctgggcgg cgacggtgcc ggtgacggcg aacagggcgg cagccccgat
     9001 cgatgcgcga atgagcgcct tatgcatggt gttcctctcc caaatcgggg cgttctaacg
     9061 gccccctagg ccaactggca catcgcgcgc catcggcgag ccccgcgaac gggacggctc
     9121 gagaccgtcc gttgaaggcg tcaatttgtc aagtaaaccg acgcaaatag cgtcacctcc
     9181 gacaaagcgg tcgtgcgcgg atgtagcatc gctatagtcg ggatcggatc acggctgcgg
     9241 gatcgacatg gcctcctttg cctttgcgga taatctcatc ctcatcttca aggtgacggt
     9301 gacgcccctc ctcgtggcgc tcgcatcgct ggctgcccgg cgctggggac cgcgggtcgg
     9361 cggcatcctg atcggcttcc cggtcatgac cgggccgatc gccctcttcc tggccctcga
     9421 gcagggcgtg gcgtttgcgg cacgagcgtc ggtcggcatc ctgctcgcgc tgatcgctgt
     9481 cgccggctgg atcgtcatct acgcctggct ggcgccgcgc gcgcgctggc ctcttgcact
     9541 cgccctcgcc tcggttggct atgtgctccc gggatactgg ctggccgggc tcgagacgcg
     9601 gccggcggtt gcggcggcgc tcgcgattgc agccattctg gcggcgatcg tcgcgatcgg
     9661 tcacccgcgc ggtgccagga tcaccggacc agtgccctgg tgggacatct ggctgcgcat
     9721 gctcgtgagc gccacgctcg tcaccggcat cacgctggct gccgagcgcc tcgggccgat
     9781 ggcgagcggc atcgtcggca cattccccgc gatctctacg gtcgtcgtga ccttcacgca
     9841 ccatcgctgg ggtgcggggg ctgcgacggc catcatgcgc ggcctgctgc tgtcgctcct
     9901 gtcgtttgcg agctgcttcc tggtgatcgg cgagttgatc atctcgcacg gcatcgccgt
     9961 atcgttcctg ctcggcgtgc tcgccggtgg tgtgacgagc cttgcggtgc tttctgcgga
    10021 cggctggctg cgcagatcga gtgagtcatg acagatcacg gatcgatcac gaaaagttgc
    10081 ggagcacttc tgccgcaatc atcgtctata attcatcctg gccgaatgcg gccgacgtgg
    10141 ttggctagga gatgcgaatg atgaagtcga aggctttgtt ggccgttgcg gtgattgccg
    10201 gcgcggcgat cggtgcgagt gcactcgtca acgcacaaga cgtgatcgcg cagcgcaagg
    10261 cgctcatgaa ggcgaccggt ggcgcgttca agacggcgac cgacatggcc aagggcgagg
    10321 cgcccttcga cgccgccaag gcttcggcag ccatgaagaa gatcgccgat gattggcccg
    10381 tcttcatgaa gctcttcccg gacaactcga agaccggcgg tgaaacaacg gcgcacggcg
    10441 acatctggac gaagcgcgcc gaattcgnnn nnnnnnnatg ccaagggcgc ggcgatggtg
    10501 aaggcggtga cggccgccgg tgaagctgcg gccaagggcc tcgacgcgtt caagacggcg
    10561 catgccgaga tcggcaagaa ctgcggcggc tgccacacgc ccttccgcgt caagaagaac
    10621 tgacgagctt tcctcgtcgt gaaccgcagg cggaggaggc gagtgtctcc tccgcctttt
    10681 ttgtgcgtcg catgttgcga gacttgagcg ggccaccggc gacgctcggc cgcgctgcga
    10741 tcatcagtcc tgcgcgcgct tctcgagcgc ttccttgagg gcgctgagct tggcgaaggg
    10801 cgaatcgggg tcgaggccct ggcgctttgg cggcgccgcc gtgcgcgtct gagacggcgg
    10861 ccggtcgtcg cggccccg
//
LOCUS       DAORNH010000138        10809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4564_length_10809_cov_2.806119, whole genome shotgun sequence.
ACCESSION   DAORNH010000138 DAORNH010000000
VERSION     DAORNH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4564_length_10809_cov_2.806119"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK264_14125"
     CDS             <1..505
                     /locus_tag="PK264_14125"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRD77044.1"
                     /translation="GVQVPHVNTADDARAAVAAVKFGPGAARGLAAGTRPDRWGLGAR
                     MPDFVAAANAQSFVCVQIEHAEAVANAAEIAAVPGVDVVFIGPSDLSQSMGHPGNPKA
                     PAVAAAIRQTLAVIRDSGKAPGMPATSDTLGEVLGEGCRYIYTHLPRILGAGARLFLD
                     AGKSRKS"
     gene            517..1176
                     /locus_tag="PK264_14130"
     CDS             517..1176
                     /locus_tag="PK264_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77045.1"
                     /translation="MSKPDASGGLLKAAVVPVTPFQQNCTLIWNHGTKVGVVIDPGGD
                     LDIIEDAIRQVGMTVEKIILTHGHIDHAGGADELRAKLKVKIEGPHKADKFLLDGLAE
                     QAKRFGVDSVRPVTPDRWLDEGDSVTIGGETFEIFHCPGHSPGSVVFFHRRQRLALVG
                     DVLFRGSIGRTDFPYGDHDALISAIKTKLLPLGDDVAFICGHGPTSSFGRERETNPFI
                     Q"
     gene            1747..5460
                     /locus_tag="PK264_14135"
     CDS             1747..5460
                     /locus_tag="PK264_14135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HRD77046.1"
                     /translation="MKISRRFTEAGKSPYDKIPFRRATSEIKNPDGSIVFRLEGFAVP
                     EHWSQVAADIIAQKYFRKAGVPRILRRVEETQVPSWLWRSVADERANAQLPEGERATG
                     ERDARQVFDRLAGTWTYWGWKGGYFSSEEDARSFFDELCYMLAMQYGAPNSPQWFNTG
                     LHWAYGIDGPGQGHFFVDPVTKELTASTSAYEHPQPHACFIQSIEDDLVNENGIMDLW
                     VREARLFKYGSGTGTNFSKLRSENERLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVVVDVDHPDIEDYIDWKVREEQKVAALVTGSLICQKRLKAVMSACVNCEADGDA
                     CFDPAKNPALKRAIRDAKRDQVPQNYVLRVIQFAKQGFKDISFDTYDTDWDSEAYRTV
                     SGQNSNNSVRVTDEFLSAVETGGDWNLTARTSGKVTKTLKARELWEKIGHAAWASADP
                     GIQFHTTINDWHTCPKSGPIRASNPCSEYMFLDDTACNLASLNLMKFRQADGSFDVAA
                     YEHACRIWTIVLEISVMMAQFPSRQIAELSYRYRTLGLGFANIGGLLMASGIPYDSAE
                     GRAICGAISAVMTGVAYATSAEMAAELGAFPGYPANADDMLRVMRNHRLAAYGQREGY
                     EALANAPVPLDHAALADKGLLAAATRAWDRALELGMAHGYRNAQATVVAPTGTIGLVM
                     DCDTTGIEPDFALVKFKKLAGGGYFKIINQAVPEALRTLGYRASEIAEIEAYAVGHGN
                     LKQAPHINASSLKRKGFTDEIIAKIDRALGPAFDIKFVFNRWTVGDEFLTKTLGIPAE
                     KLAEPTFDLLTHLGFTKAEIDAANDHICGSMTVEGAPFLRKEHLAVFDCANPCGRTGK
                     RFLSVESHIRMMAASQPFISGAISKTINMPNEATVEDCKSAYMLSWRLALKANALYRD
                     GSKLSQPLNSQILGSDADEQEAAAEKLAADKPATQRATVAAERIVERIIEKARERDKL
                     PGRRKGYTQKASVGGHKVYLRTGEYEDGRLGEIFIDMHKEGAAFRSLMNNFAIGISLG
                     LQYGVPLEEYVDAFTFTRFEPAGLVHGNEAIKNATSILDYIFRELAVSYLGRNDLAHV
                     DPSEIVGATSLGSSDEALEDEAEAAAAQPPAAKFLSHGLVRGIVKPTAMKLAVGAEGG
                     PVGLLQTEVATAFVRTETETGPSLYSSRVVAEETSTQSLLTLKRETKAERAAAAKIQG
                     YEGEACGQCGNFTLVRNGTCLKCNTCGGTSGCS"
     gene            5632..5862
                     /locus_tag="PK264_14140"
     CDS             5632..5862
                     /locus_tag="PK264_14140"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HRD77047.1"
                     /translation="MALDRYEIRHNDGHWAFIKVGGQRATRTFEKKTDAVKYASEFGK
                     THDFSLRIKDESGKIQEERTYPRSKDPKSSKG"
     gene            6023..6373
                     /locus_tag="PK264_14145"
     CDS             6023..6373
                     /locus_tag="PK264_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77048.1"
                     /translation="MASRLPRHEAALNDIARPAATGFLRMGRRPQSAHGDREPLVAAE
                     LVGTVRARLGLDSAIPDREIEACIGAIMQRSDARGRWLAAFSSDLGRSLIAAGRDLNT
                     MLRVLIALAPRARS"
     gene            6384..7001
                     /locus_tag="PK264_14150"
     CDS             6384..7001
                     /locus_tag="PK264_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77049.1"
                     /translation="MSNATRPAAPPEIHFLIDHFDVIVAAGAELLSCSYSAQSAAVGE
                     AGERARVQGAGRSRFLADVARLEAIIVGRLVQARRWASEAGGADVNARSVVRLFLAAT
                     AEIGEQRATGQRPGLNVGNDPFVYMRSRALIARDAAGTSLLGRIDVTPAFAIWGRIPL
                     GALMEVVSAARNALAMAYGLASARGEEETAEPTFDVLLAELRKAS"
     gene            complement(7011..8276)
                     /locus_tag="PK264_14155"
     CDS             complement(7011..8276)
                     /locus_tag="PK264_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD77050.1"
                     /translation="MAVKAKSKTTETAPSDVAGTTALAGVKVVDLTQFEAGTSITETL
                     AWLGADVIKIENPIGGEQGRGASTDRKGVDSYYFMLLNANKRSVTLNLKDPRGKEMLR
                     QLIAKADIFAENFGPGVIEKLGFSYEEVARINPRIIYATVKGFGRGSPFEKYLSFDMI
                     GQATGGVMSITGEADGKPIKPGVTLGDTGTGLHACIGVLAALYQREATGRGQKIEVAM
                     QDAMVNYCRIAYAAQLLHDKPCPRMGNQVVLGTNAPSDVFKCEPGGPNDYVYIYTSRA
                     NNVQWERLLDVIGRSDLKNDPRMATPQLRVKIVDEINGHIQAWVKNYSKHEAMRRLGE
                     AGVPCGAVLDTEELMNDRSMREREIFVEVDHPVRGKVVIPGWPVKMTGSHVPVTCSPL
                     LGADNGAVYADWLGLSEADLAKLRGDGVI"
     gene            8405..8911
                     /locus_tag="PK264_14160"
     CDS             8405..8911
                     /locus_tag="PK264_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRD77051.1"
                     /translation="MISVEHVRTMARYNRWQNRSLYAAADSLTDAQRRESRGAFFGSV
                     HGTLSHLMWGDRLWMARFEGKPRPHTLPVTESPTYISDWAAMKAERASEDQRIIAWAD
                     GIDPASLYRDLTWTSGIAGSERTRPLWLLITHFYNHQTHHRGQVHALLTGLGAKPDDT
                     DLIIMPAA"
     gene            8949..9452
                     /gene="coaD"
                     /locus_tag="PK264_14165"
     CDS             8949..9452
                     /gene="coaD"
                     /locus_tag="PK264_14165"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRD77052.1"
                     /translation="MQRIGFYSGSFDPVTLGHIDVIRRALKLVDSLVIGIGVHPGKAP
                     TFSVDERAEILERETALARAANAKVKVVTFAGLTVDAARAAGAAAIFRGLRDSTDFDY
                     EMQMSGMNGVMADDIDTVFLPASPQVRHITATLVRQIAFMGGDVSGFVTKDVARRLKA
                     KVKAAQK"
     gene            9476..10144
                     /gene="pdxH"
                     /locus_tag="PK264_14170"
     CDS             9476..10144
                     /gene="pdxH"
                     /locus_tag="PK264_14170"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRD77053.1"
                     /translation="MPNDPTGETKKPFYSGPEAGDFTSADEPFTLFEAWLAEAEKHEP
                     NDPNAMAVATADGDGLPNVRMVLLKGLDPAGHAARGFVFYTNFESAKGRELLAARKAA
                     LLFHWKSLRRQVRVRGLVSVVSDGEADQYFASRHRSSRIGAWASQQSRPLESRFALEK
                     AVAYYGARHVVGEVPRPAYWSGFRITPLEIEFWHDRPFRLHDRVVMRRADSGEPWSRT
                     RLYP"
     gene            10152..>10809
                     /locus_tag="PK264_14175"
     CDS             10152..>10809
                     /locus_tag="PK264_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRD77054.1"
                     /translation="MRIGIDLGGTKIEGVALGADGTERHRIRIASPRGSYEATIAAIR
                     EVVEGLEATAGAAASIGVGMPGSISPRTGRVQNANSTWLNGRTFSVDLPAALGRPVAF
                     ANDANCFALSEAVDGAGAGARIVLGIILGTGCGSGIVVDGRILDGPRGIGGEWGHNPL
                     PWAAADEHPGTRCWCGRLGCMETWVSGPALTDDHRRVTGGAATGEEIVARAAGGDPAA
                     K"
BASE COUNT         1837 a         3586 c         3568 g         1818 t
ORIGIN      
        1 gggcgtacag gtgccgcacg tgaacacggc cgacgacgcc cgcgcggccg tcgcggcggt
       61 gaagttcggc cccggtgccg cgcgcggcct tgctgccggc acacgccccg accgctgggg
      121 acttggcgcg cgcatgccgg acttcgtcgc ggcggccaat gcccagtcgt tcgtctgcgt
      181 gcagatcgaa cacgccgagg cggtggcgaa tgctgccgag atcgcggccg tgccgggcgt
      241 ggacgtcgtg ttcatcgggc cttccgatct ctcgcagtcg atgggccacc ccggcaatcc
      301 gaaggcgccc gcggtcgccg cggcgatccg ccagacgctc gctgtcatcc gcgactcggg
      361 caaggcaccc ggcatgccgg caacgtccga cacgctcggc gaggtgctgg gcgagggctg
      421 ccgctatatc tacacacacc tgccgcgcat cctcggtgcc ggcgcacgcc tgttcctcga
      481 cgcgggcaag tcccgtaaat cctgaaggat cacgtcatgt cgaagccaga cgcatcgggt
      541 gggctcctca aggcggccgt cgtaccggtg acgccgttcc agcagaactg cacgctcatc
      601 tggaatcacg gcaccaaggt cggcgtcgtg atcgatccgg gtggcgatct cgacatcatc
      661 gaggatgcca tcaggcaggt cgggatgacg gtcgagaaaa tcatcctgac gcatggccat
      721 atcgatcacg cgggcggcgc cgatgagctg cgcgcgaagc tgaaggtcaa gatcgagggg
      781 ccgcacaagg ccgacaagtt cctgctcgat ggcctcgccg aacaggccaa gcgcttcggc
      841 gtcgacagcg tgcgacccgt gacgccggat cgctggctcg acgagggcga cagcgtgacg
      901 atcggcggcg agaccttcga gatctttcat tgcccgggcc actcgccagg ctcggtcgtg
      961 ttcttccatc gccgccagcg gctggcgctc gtcggcgacg tactcttccg cggctctatc
     1021 gggcggaccg attttcccta cggcgatcac gacgcgctca tcagcgcgat caagaccaag
     1081 ctattgccgc tcggcgacga tgtggccttc atttgcgggc acggcccgac aagcagcttc
     1141 ggccgcgagc gcgagaccaa tccgttcatt cagtgatgtg gagcttcggt tggccggcac
     1201 gacagaaggc gctgccgccg caggtcttgc gatgctcatc ggcaacacct atcgcgcgag
     1261 gccccttggc ataccgcaaa ccggctcgaa atcttcctcg cgagcacacc gctcacccac
     1321 acatcgccgg tcgggatgtg aattctcgcg tgggttccgg cggatgccga gttgaccggg
     1381 tgagcaacgt tttgccccac aggatttcca cgggtcgtcc acgtttagac cgcaacatct
     1441 agtattgcgt tgaacgtaca cccccaagag ttcgtggcgg ggcggctcga atcgctgtag
     1501 ctttttcctt gtcgggagtg aggccggccg ctcgctgaaa agcagctgcc gcccaaggcg
     1561 gatagccggt ttcggccgga gttccccata cccttgtgag tagcgttcgg tcgtgcggcc
     1621 agtcgcccag tcggggactg cgcgagcatg gcactgttgc gtcgagtcat caccgaaaat
     1681 ccaagaaacg gcaatgcgcg cgcccttgcg gggtgacgcg tgtgcgatca cgcgggggac
     1741 tgacagatga aaatttcgcg ccgcttcacc gaggccggca agtcgccgta cgacaagatc
     1801 ccgttccgcc gcgcgaccag cgagatcaag aaccccgacg gctcgatcgt cttccgcctc
     1861 gagggcttcg ccgtgcccga gcactggagt caggtggcgg ccgacatcat cgcgcagaag
     1921 tatttccgca aggccggcgt gccgcgcatc ctgcgccgcg tcgaggaaac gcaggtgccg
     1981 tcctggctct ggcgctccgt cgccgacgag cgcgccaacg cgcagctccc cgagggcgag
     2041 cgcgcgaccg gcgagcgcga tgcgcgccag gtcttcgacc gcctcgcagg cacgtggacg
     2101 tactggggct ggaagggcgg ctacttctcg agcgaggagg atgcccgcag cttcttcgac
     2161 gagctatgct acatgctcgc catgcagtac ggcgcgccga actcgccgca gtggttcaac
     2221 acggggctcc actgggccta cggcatcgac ggccccggcc agggccactt cttcgtggac
     2281 ccggtgacga aggagctcac tgcctcgacg agtgcctacg agcatcccca gccgcatgcc
     2341 tgcttcatcc agtccatcga ggacgatctc gtcaacgaga acggcatcat ggacctgtgg
     2401 gtgcgcgagg cgcgcctttt caagtacggc tcgggcacgg gcacgaactt ttccaagctc
     2461 cgctccgaga acgagcggct ctcgggtggc gggcgctcgt cgggcctcat gagcttcctg
     2521 aagatcggtg accgcgcggc tggcgccatc aaatcgggcg gcaccacacg ccgcgccgcg
     2581 aagatggtgg tcgtcgacgt cgatcatccg gatatcgagg actacatcga ctggaaggtg
     2641 cgtgaggagc agaaggtggc ggccctcgtc acgggctcgc tcatctgcca gaagcgcctg
     2701 aaggccgtca tgtcggcctg cgtcaactgc gaggcggacg gcgacgcctg cttcgatccg
     2761 gccaagaacc cggcactcaa acgcgccatc cgcgatgcca agcgcgatca ggttccgcag
     2821 aactacgtcc tgcgcgtcat ccagttcgcc aagcagggct tcaaggacat ctccttcgac
     2881 acctacgaca ccgactggga cagcgaggcg tatcgcaccg tatcgggtca gaactcgaac
     2941 aactccgtgc gcgtgacgga cgagttcctc tccgcggtcg agacgggcgg cgactggaac
     3001 ctcacggccc gcacgagcgg caaggtgacg aagacgctga aggcgcgcga actctgggag
     3061 aagatcggcc acgccgcttg ggcgtccgcc gatcccggca tccagttcca cacgaccatc
     3121 aacgattggc acacctgccc gaagagtggc ccgatccgcg cctcgaaccc gtgctccgag
     3181 tacatgttcc tcgacgacac ggcctgcaac ctcgcctcgc tgaacctcat gaagttccgc
     3241 caggcggacg gtagcttcga cgttgcagcc tacgagcacg cctgccgcat ctggacgatc
     3301 gtgctcgaga tctcggtgat gatggcgcag ttcccgagcc gccagatcgc cgagctctcc
     3361 tatcgctacc gcacactcgg gctcggcttc gccaacatcg gcggcctcct gatggccagc
     3421 ggcattccct acgatagcgc cgaaggccgc gcgatctgcg gcgcgatctc ggccgtcatg
     3481 accggtgtcg cctacgcgac gtcggccgag atggcggccg aactcggtgc gttccccggc
     3541 tacccggcga acgcggacga catgctgcgg gtcatgcgca atcacaggct tgcggcctac
     3601 ggccagcgtg agggctacga ggcgctcgca aatgctccgg tgccgctcga tcacgcagcg
     3661 ctcgccgaca aggggctgct tgccgccgcg acccgcgcct gggatcgggc cctcgagctc
     3721 ggcatggcgc acggctaccg caatgcgcag gcgacggtgg tcgcgccgac cggtacgatc
     3781 ggcctcgtca tggactgcga tacgaccggg atcgagcccg acttcgcgct cgtgaagttc
     3841 aagaagctgg ccggcggcgg ctacttcaag atcatcaacc aggccgttcc cgaggcgctg
     3901 cggacactcg gctatcgcgc cagcgagatc gccgagatcg aagcctacgc tgtcgggcat
     3961 ggcaacctga agcaggcgcc gcacatcaac gcatcgtcct tgaagcgcaa gggcttcacc
     4021 gacgagatca tcgccaagat cgaccgcgcg ctcggcccgg ccttcgacat caagttcgtc
     4081 ttcaaccgct ggacggtcgg cgacgagttc ctgacgaaga cgctcggcat tccggcggag
     4141 aagctcgccg agccgacatt cgatctgctc acacatctcg gcttcacgaa ggccgagatc
     4201 gacgcggcca acgatcacat ctgcgggtcg atgactgtcg aaggcgcgcc gttcctcagg
     4261 aaggagcacc tcgctgtgtt cgattgcgcc aacccttgcg gccgcacggg caagcgcttt
     4321 ctctccgtcg agagccacat ccgcatgatg gcggcgtcac agccgttcat ctcgggtgcc
     4381 atctcgaaga cgatcaacat gccgaacgag gcgacggtcg aggactgcaa gtcggcctac
     4441 atgctctcct ggcggctcgc cctcaaggcg aatgccctct accgcgacgg ctcgaagctc
     4501 tcccagccgc tcaactcgca gatcctcggc agcgatgccg acgagcagga ggctgcggcc
     4561 gagaagctcg cggccgacaa gccggcgacg cagcgtgcga cggtggcggc cgagcggatc
     4621 gtcgagcgca tcatcgagaa ggcacgcgag cgcgacaagc tgccgggcag gcgcaagggc
     4681 tatacgcaga aggcctccgt cggcgggcac aaggtgtacc tgcgcacggg cgagtacgag
     4741 gacgggcggc tcggcgagat tttcatcgac atgcacaagg agggcgccgc cttccgcagc
     4801 ctgatgaaca acttcgccat cggcatctcg ctcggcctcc agtacggcgt gccgctcgag
     4861 gagtacgtgg acgccttcac gttcacgcgc ttcgagccgg cagggctcgt tcacggcaac
     4921 gaagcgatca agaacgccac gtcgatcctc gactacatct tccgcgagct cgccgtctcc
     4981 tatctcggcc gtaacgacct ggcccacgtc gatccctccg agatcgtcgg cgcgacctcg
     5041 ctcgggtcct cggacgaagc actcgaggac gaggccgagg cagcagctgc gcaaccgccg
     5101 gccgccaagt tcctctcgca cggccttgtc cgcggcatcg tcaaaccgac ggcgatgaag
     5161 ctcgcggttg gtgccgaggg tggtcccgtg ggtctgctgc agaccgaggt cgcgaccgcg
     5221 ttcgtgcgca ccgagacgga aacgggtccg agcctctatt cgtcgcgcgt cgtcgccgag
     5281 gagacatcga cgcagtcgct cctcaccttg aagcgcgaga cgaaagccga gcgcgcggca
     5341 gcggcgaaaa tccagggcta cgagggcgag gcctgcggcc agtgcggcaa cttcacgctc
     5401 gtgcgtaacg gcacctgcct gaagtgcaac acctgtggcg ggacgagcgg ctgcagctga
     5461 gttcgatcag tcatagtcag ctcggccggc agcccctcga cgctgccggc cgaatcattc
     5521 gaagctcttt ccgtatcgac gagtatggcc tgcctctggc actgtgcgcc ttacatgtcg
     5581 ggacgcgtac tgcatagtgc ggtcgcccgg catgacgaag aggagatgac gatggcactg
     5641 gaccgttacg agatccgcca caacgatggc cactgggcct tcatcaaggt cggcgggcag
     5701 cgcgcaactc gcaccttcga gaagaagacc gacgccgtga agtatgcgtc cgagttcggc
     5761 aagacgcatg atttctcgct ccgcatcaag gacgagagcg gcaagatcca ggaggagcgg
     5821 acctatccgc gcagcaagga tccgaagagc agcaagggtt gagtgacggc cgccatcccg
     5881 tgcgtcagcg aagggttccg cggcgctgcc tgcggcggcg atatgtccgc cgacgactgt
     5941 ttaagtgatc agttgcttcg ttgcgttgcg tttctggtcg ccgcgatccc aattcggggt
     6001 gcggcagtgg agacaaggcg ccatggcttc tcgattgcct cgccacgagg cggcactgaa
     6061 tgacatcgcc cgtcccgccg cgacgggctt cctgcgtatg ggtcgtcgac cgcagtcggc
     6121 acatggcgac cgcgaaccgc tcgtcgcagc cgagctcgtc ggcacggtga gggcgcggct
     6181 cggcctcgac tcggcgattc ctgatcgcga gatcgaggcc tgcatcggcg ccatcatgca
     6241 acgctccgat gcgcgcggcc gttggctcgc ggccttttcc tccgatctcg gccgctcgct
     6301 gatcgcagcc ggacgtgacc tcaacacgat gttgcgcgtg ctgatcgccc tcgctccgcg
     6361 cgcgcgctcg tgaggggacg gccatgtcga acgccacgcg ccctgcagcg ccgcccgaga
     6421 tccatttcct catcgaccac ttcgacgtga tcgtcgccgc tggcgccgag ttgctctcct
     6481 gctcctactc ggcacagtct gcggcagtgg gcgaagcggg ggagcgcgcg cgcgtccaag
     6541 gggccggtcg atcgcgcttc ctcgccgatg ttgcgcggct cgaagccatc atcgtcggca
     6601 ggctcgtgca ggctcgacgc tgggcgagcg aggcgggcgg cgctgacgtg aatgcacggt
     6661 ccgtcgttcg cctcttcctt gccgcgaccg cagagattgg tgagcagcgt gccaccggac
     6721 agcgccccgg gctcaatgtc ggcaacgatc ccttcgtgta catgcgcagc cgcgccctca
     6781 tcgcgcgtga tgcggccggt accagtctcc ttggtcggat cgacgtgacg ccggccttcg
     6841 cgatctgggg acgcatcccg ctcggggcgc tgatggaggt ggtcagcgcg gcgcgcaatg
     6901 ccctcgcaat ggcatacggt cttgcgtccg cccgtggcga ggaagagacc gcggagccga
     6961 ccttcgatgt cctgctcgcg gagctccgca aggcctcgtg acggtcggtg tcagatcaca
     7021 ccgtcgcctc tgagcttcgc gaggtcggcc tcggagaggc cgagccagtc cgcgtagacc
     7081 gctccgttgt cggcaccgag gagaggcgag cacgtcacgg gaacgtggct gccggtcatc
     7141 ttcaccggcc agccggggat tacgaccttg ccgcgcacag ggtgatccac ctcgacgaag
     7201 atttcgcgct cgcgcatcga gcgatcgttc atgagctctt cggtatcgag cacggcgccg
     7261 cagggaacac ccgcctcgcc gaggcggcgc atcgcctcgt gcttggagta gttcttcacc
     7321 cacgcctgga tgtggccgtt gatctcatcg acgatcttga cgcgcaattg cggtgtcgcc
     7381 atgcgcgggt cgttcttcag gtccgaccga ccgatgacgt cgagcagccg ctcccactgc
     7441 acgttgttgg cgcggctcgt gtagatgtag acgtagtcgt tcggcccgcc cggctcgcac
     7501 ttgaagacgt cggagggcgc gttggtaccg agcacgacct ggttgcccat gcgcgggcag
     7561 ggcttgtcgt gtaggagctg cgccgcatag gcgatgcggc agtagttcac catggcgtcc
     7621 tgcatggcga cctcgatctt ctgcccgcgt ccggttgcct cacgctgata gagcgccgca
     7681 agcacgccga tgcaggcatg aagcccggtg ccggtgtcgc cgagcgtgac gcccggtttg
     7741 atcggcttgc cgtcggcttc gcccgtgatg ctcatgacgc cgccagtcgc ctgaccgatc
     7801 atgtcgaacg acaagtactt ctcgaaaggg ctgccgcggc cgaagccctt cactgtcgca
     7861 tagatgatgc gcgggttgat gcgtgcgacc tcttcgtagg aaaagccgag cttctcgatt
     7921 acgcccggcc cgaagttctc ggcaaagatg tcggccttgg cgatcagctg gcgtagcatc
     7981 tccttgccgc gcgggtcctt cagattgagc gtcaccgaac gcttgttggc gttgagcagc
     8041 atgaaatagt agctgtcgac gccctttctg tcagttgagg cgccgcggcc ctgctcgccg
     8101 ccgatcgggt tctcgatctt gatgacgtcg gcgcctagcc aggcgagcgt ctcggtgatc
     8161 gaggtgccgg cctcgaactg ggtgagatcg acgaccttga cgccggcaag tgccgtggtg
     8221 ccggccacat ccgagggcgc ggtctcggtc gttttggatt tcgccttcac ggccatggac
     8281 gtctccacct tcggactttt tcctttccgc cattgagcat tgtctttgcc tccgccacaa
     8341 tgggggagag atgcagacga ggcgggcgcg cgatgcagag cggctcggcc gggagtaggt
     8401 gccaatgatc tccgtcgagc acgtgcgcac gatggcgcgc tacaatcgct ggcagaaccg
     8461 atcgctctat gctgccgcgg acagccttac cgacgctcag cgccgcgaga gccgcggcgc
     8521 cttcttcggc tcggtccacg gcacattgag ccacctgatg tggggtgatc gcctctggat
     8581 ggcgcgcttc gagggtaagc cgcggccgca caccctgcca gtgaccgaga gcccgacata
     8641 catcagtgac tgggcggcca tgaaggccga gcgcgcgagc gaggaccagc gcatcattgc
     8701 ctgggcggac gggatcgatc cggcctcgct gtaccgcgat ctcacctgga cttccgggat
     8761 cgccggaagc gagagaacgc gcccactctg gctgctcatc acccacttct acaatcatca
     8821 gacgcatcat cgcgggcagg tgcatgctct attgaccggt ctgggcgcca agcccgacga
     8881 taccgatctc atcatcatgc ccgccgcctg aggcacgggc acgctgacca cgcgaggctc
     8941 agagagacat gcaacgcatc ggcttctact ccggctcctt cgatccggtg acgctcggcc
     9001 atatcgacgt gatccggcgg gctctgaagc tcgtcgacag ccttgtcatc ggcatcggcg
     9061 tgcatcccgg caaggcaccg acgttctcgg tcgatgagcg cgccgagatc ctggagcgcg
     9121 agacggcgtt ggcccgggcg gcgaatgcca aggtgaaggt cgtcacgttc gcggggctca
     9181 ccgtcgacgc tgcccgcgcg gcgggcgccg ccgctatctt tcgcggtctt cgcgacagca
     9241 ccgacttcga ctacgagatg cagatgtcag gcatgaacgg cgtcatggcc gatgacatcg
     9301 atactgtctt tctgccggcg agtccgcagg tgcggcacat cacggcgacg ctcgtccgac
     9361 agatcgcatt catgggcggc gacgtatcgg gcttcgtcac caaggatgtg gcgcggcgcc
     9421 tgaaggccaa ggtcaaggcc gcgcagaagt gagcgccgcg agaaggaact gacgcatgcc
     9481 gaacgatccg acgggcgaga cgaagaagcc gttctactcg gggccggaag ccggagattt
     9541 cacaagcgcg gacgagccat tcacgctgtt cgaagcatgg ctcgccgagg ccgagaagca
     9601 cgagccgaac gatccaaacg ccatggcggt cgccaccgcc gacggggacg gattgccgaa
     9661 cgttcgcatg gtgctcttga agggcctcga tccggccggt catgccgcgc ggggcttcgt
     9721 cttctatacg aacttcgaga gcgccaaggg gcgcgagctg ctggccgcgc ggaaggcagc
     9781 actcctcttc cactggaaga gcctgcggcg gcaggttcgc gtgcgagggc tcgtgagcgt
     9841 cgtctcggac ggcgaggccg accagtattt cgcctctcgt caccgctcga gccgcatcgg
     9901 cgcctgggcc tcgcagcagt cgcgcccgct cgaaagccgc ttcgcgcttg agaaggccgt
     9961 tgcctactac ggtgcgcggc acgtggtcgg cgaggttccg cggccggcct actggagcgg
    10021 gttccgcatc acgccgctcg aaatcgagtt ctggcacgac cggcctttcc gcctgcacga
    10081 ccgcgtggtg atgcggcggg cggattctgg cgagccctgg tcgcggacgc ggctctatcc
    10141 ttgagtgcgc catgcggatc ggcatcgacc tcggcggaac gaaaatcgag ggcgtggcgc
    10201 tcggagctga tggcacggag cggcatcgca tccggatcgc ctcgccgcgg gggagctacg
    10261 aggcgacgat cgccgccatc cgcgaggtgg tcgagggact agaggccacc gcgggtgcgg
    10321 cggcctcgat cggcgtcggc atgccaggct cgatctcgcc gcgaacgggc cgcgttcaga
    10381 atgccaactc gacgtggctc aacggtcgca ctttctcggt tgatctgccc gcggccctcg
    10441 gccggccggt cgccttcgcc aatgatgcca actgtttcgc gctctcggag gcggtcgacg
    10501 gggccggcgc cggcgcgcgc atcgtgctcg gcatcatcct cggcacgggc tgcggcagcg
    10561 gcattgtcgt cgacgggcgc atcctcgacg gaccgcgcgg catcggcggc gagtggggac
    10621 acaatccgct tccctgggcg gcggcggacg agcatccggg cacgcgctgc tggtgcggta
    10681 ggctcggctg catggagacg tgggtgtcgg gcccggcgct caccgacgac caccggcggg
    10741 tcacgggcgg agccgcgacg ggtgaggaga tcgtcgcgcg tgccgcaggc ggcgatccgg
    10801 cagcgaagg
//
LOCUS       DAORNH010000139        10799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4567_length_10799_cov_2.491623, whole genome shotgun sequence.
ACCESSION   DAORNH010000139 DAORNH010000000
VERSION     DAORNH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4567_length_10799_cov_2.491623"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2178)
                     /locus_tag="PK264_14180"
     CDS             complement(<1..2178)
                     /locus_tag="PK264_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRD77055.1"
                     /translation="MTDADGYGSSGRWSRSPSSGRVEPLLVDDIVLAVANHAYDVALA
                     HGAGEVRLDHLVHALTRVDAAAQILERRGIREGQLRRDSAALIANDAPIGMSGDRLPP
                     RRSEDIEDVLRRASSSAARRGEVTSVEDILWVLLTHPRDNAAISLLRVHAPDWHRSDW
                     VRIRDRQPRIELRNEPRLEYRPEPRPAPRLETVVVQDPFGSRLDGVEGSLRSLHGELA
                     IDRKTIGDLIRELQRDIAAHRTDVAALRGDIGDRMQALERTALARPEPARMPTQLSER
                     MQGIEVKLADLMRLWSLSSERLQSVERAVDTRVAEAQRVPTMVAERIETLESNITARL
                     ASLPDPAASLAGHMTQLSDRLASVEVALNERTAAAQDLVMVAERVQTLEKSVHSGLGE
                     GARNWSALAQRLQSIEAAVARGGDTAPLGDRLTAIERALADRLAGTERLVDQRTAEAQ
                     RGTALIADRLLAIEKSIRTGAGGDATTLWPQLGARLQTLERMIETRASEATSHSRVLG
                     DRLKTIEDQVAAQRAENVQTRATIGAQLKSFEAGLGSFTPAGIDVSGLQSTLAERFQV
                     LKRDLDAERSAVTAPVIQRLAQVEAQTAARHNELANAVGLFVQRVGGLEQSVQTTLKG
                     LAEGSTTQARDLAEVQEVVLQLTEHQNAVTQSLAEWREETRGDLSIINNQLRELITHR
                     GTIAVAPTASPSVAPSPPPAVVAASAIVAPDAAPQPAAKRNGSG"
     gene            complement(2518..>2761)
                     /locus_tag="PK264_14185"
     CDS             complement(2518..>2761)
                     /locus_tag="PK264_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HRD77056.1"
                     /translation="ILTTAANATVGRIHDRMPVILAPEAFERWLDCRKVPPAEVADLL
                     VPAPEDLLTITEIDRRIGNPRHEGPDLILPQKQTLL"
     assembly_gap    2762..2771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2772..3227)
                     /locus_tag="PK264_14190"
     CDS             complement(<2772..3227)
                     /locus_tag="PK264_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HRD77057.1"
                     /translation="MCSRYNLTSPHEAVRAYFGYRTDAEFPPRYNIAPSQPVAIVRLS
                     ADNVARELALVRWGLIPSWVKDPREFSTLINARSETAAAKPSFRGSMRHRRCLIPANG
                     FYEWTGPAGHKQPHLLARHDGGILAFAGLHEHWLGADGSELETACIVTTT"
     gene            complement(3236..4054)
                     /locus_tag="PK264_14195"
     CDS             complement(3236..4054)
                     /locus_tag="PK264_14195"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77058.1"
                     /translation="MRILLRMVVALLAAASCALSADVALAAERCIGMAEAPGRGGTPA
                     IIRTSFREVNLKRTEVRITYVGHSTFVIESAAGIRAATDYNDYVRPAELPDVVTMNRA
                     HNTHYTDVPDPRIKHVLRGWDPKGGAAAHDVTVSDMHVRNVATNIRYGGETYEFGNSI
                     FIFEVADLCIGHLGHLHHTLTTQQLAQIGQLDIVLVPVDGSFTMDLDGMMETLKTLKA
                     RLMLPMHYFSPYTLERFLTRARAEFEVEIEARPTIVISRATLPKRPTLRVLPDR"
     gene            complement(4147..4788)
                     /locus_tag="PK264_14200"
     CDS             complement(4147..4788)
                     /locus_tag="PK264_14200"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRD77059.1"
                     /translation="MRHRAISIGLLLVALLALPRALSLAAEPLPPPIGPAEPKVAPLK
                     EGSLYTQTWFVNSFLDLRDDYEQARKAGKRFAIVFEQNGCPYCVKMHTEVLAQRYIND
                     YVRTHFDVIQLDLFGAREVTDFDGRKMTEKKLAERWGVIFTPTIIFLKHDLTGLEGKF
                     GRVLEASERMPAGMGAGTFYDMFVWVRLRLYEQDPNFQRFHIARHQEREALKK"
     gene            4992..>5550
                     /locus_tag="PK264_14205"
     CDS             4992..>5550
                     /locus_tag="PK264_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD77060.1"
                     /translation="MSPRLAIVGAGALGGHVGGYLAKARRDVTLIDAWPEHVEAMRAK
                     GLTLAGLTADENFTTKVRALHITDVQGEAKRPFDVVFIAVKSYDTLWAAAMMLDYVAP
                     GGFFVSLQNGINEERLASVVGWGRTLGCIASTISVELTGPAQIQRNVMKGGAKHTVFR
                     VGEPHGRITARAKLVTELVSAADSAK"
     assembly_gap    5551..5575
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5576..6050
                     /locus_tag="PK264_14210"
     CDS             <5576..6050
                     /locus_tag="PK264_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRD77061.1"
                     /translation="GERWSKLIVNAMRNPVSAASGRGGNANDRDPVTRRAAIRIAAEA
                     IKVGLAHGYVLEDVYKMPPAQIVAAADGDTEMLAKTDAMLLEGTKSRNDDQRPSMGQD
                     IRKGRRTEIDHINGLVVAKAREAGIAVPANEAIIAAVKRVERGEAKPSPELVAGI"
     gene            6061..6405
                     /locus_tag="PK264_14215"
     CDS             6061..6405
                     /locus_tag="PK264_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77062.1"
                     /translation="MRSTHLIAALLAGLAVLAGGAGQALADAIDGDWCRDGASFRIRG
                     PSIVTPGGNAITGDYDRHGFRYVVPPSERHAGSEINMSLMNDDLVVVRRRDGATKSDM
                     EPETWRRCKVTS"
     gene            complement(6466..6804)
                     /locus_tag="PK264_14220"
     CDS             complement(6466..6804)
                     /locus_tag="PK264_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HRD77063.1"
                     /translation="MSVLNCPVCQGAMREITRNGVLIDTCTQCRGVWLDRGELEKLAS
                     IVTEPEPRSFLSGAMPARPRDDRPVERRDEHRGHWRDDDDDDDRRERRGGYGDRGPGE
                     RRRSFLDFFD"
     gene            7292..8227
                     /locus_tag="PK264_14225"
     CDS             7292..8227
                     /locus_tag="PK264_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRD77064.1"
                     /translation="MWLFFLGTVSVLLIFDLGVLHKEQREIPVSESLVLSAVYIGLGI
                     AFGAWVWWALGAASGMEYLTGFAVEKALAMDNVFVIAMIFAFFAIPSRYQHRVLFWGI
                     LGVIILRAIMIGIGATIVSQFSWVLYVFALFLIATGVKMLIVSGHGPSVEDNHVLRFM
                     RRRFNVTDQLHGEKFFVTAIDPASGKSARFMTPLFLALVLIEVADLIFAVDSVPAIFA
                     ITTDPFIVYTSNIFAILGLRALYFALAAMVHRFQHLKYALALVLIFIGSKIFIADLLG
                     IEKFPPVVALVVTFGILAAGVLFSMIKTRSPRATP"
     gene            8255..8719
                     /locus_tag="PK264_14230"
     CDS             8255..8719
                     /locus_tag="PK264_14230"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRD77065.1"
                     /translation="MAISIEDPHADSTGPHVDLPPAVTRAGHAIGAWVRIILLTVIGA
                     MTLVATVAGLIDIARAGKVTISDVLLMFIYLEIWAMIVIEATTRRLPVNFIIYIAITV
                     LTRHLVGVAGDKSTSDIGLLIDAGAILVLAPAAVLLELRFFGRQQRGEPAAR"
     gene            complement(8805..10109)
                     /locus_tag="PK264_14235"
     CDS             complement(8805..10109)
                     /locus_tag="PK264_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HRD77066.1"
                     /translation="MSTPALPRVLRFACTLAAVGVTAVSLAACSSGSTSATPEYQGLK
                     TGSVASRPDDPKAITRKDGYGSEPVRKVALLLPLSGPQQALGRQLRQAGELALFERDG
                     ARIELLVKDDRGTPDGARVAAEDAIRAGAELILGPVFSGSVAAVGPVAERNGVPVIAF
                     SNDHRVAGKGVHLLSFLPSQDVSRLVEVAARQGRKRFAVAAPDDAFGRYVEPLIEAAV
                     MRHGGVIAARSRHAGTSAGATTCAATLRQQIDEAERAGGPIDAIVVPGGPAVLQRMAQ
                     ALFAAGVDTRRIKLMGTGAWEGLSGATHPTSLGAWYAAPDVAGWRDFVGRYTKAYGMA
                     PPRLATLTFDAVSAAVVIASAPSGRRLTASDLMRPQGFPGTEGAFRLTSSGLSERALA
                     IIELTREGPQLVEAAAFPAEAKPAGPTASLYGALPYASAPSR"
     gene            10280..>10799
                     /locus_tag="PK264_14240"
     CDS             10280..>10799
                     /locus_tag="PK264_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD77067.1"
                     /translation="MSEAHSNRAALQGTVAGELDRWLDASPAVGLSLIATPIGNLGDI
                     TLRALAALATADVIYAEDTRRTLTLLNHYAIRRPVRSHHEHSSATEFAAVLADLEAGK
                     AVALVTDAGMPLISDPGFPLVREAIAKGHRVTVLPGPSAVLAALAVGGLPADTFLFAG
                     FLPPRTAGRRAAL"
BASE COUNT         1712 a         3545 c         3666 g         1841 t
ORIGIN      
        1 gccgctgccg ttgcgcttgg cggccggctg cggcgccgcg tcaggcgcaa cgatggcgct
       61 ggccgcgacc acggcggggg gcggcgacgg agccaccgag gggctcgcgg tcggggcaac
      121 ggcaatcgtg ccgcggtgcg tgatcagctc cctgagctgg ttgttgatga tggatagatc
      181 gccgcgcgtc tcctcgcgcc actcggcgag gctctgcgtc acggcgttct ggtgttcggt
      241 gagttgcagc acgacctcct ggacctcggc cagatcgcgc gcctgggtgg tgctgccctc
      301 ggcgagacct ttgagcgtcg tctgcacaga ctgctcgaga ccgccgacac gctgcacgaa
      361 gaggccgaca gcgttggcaa gctcgttgtg gcgcgccgcc gtctgcgcct cgacctgcgc
      421 gaggcgctgg atcacggggg ccgtgacggc agatcgctcg gcatctagat cgcgcttcag
      481 gacctggaag cgctcagcga gcgtcgactg gaggccgctg acgtcgatgc ccgcgggcgt
      541 gaaggagccg agccctgcct cgaagctttt caactgggcg ccgatggtgg cgcgcgtctg
      601 cacgttctcg gcacgctgtg cggcaacctg gtcctcgatc gtcttcaggc gatcaccgag
      661 cacgcgcgag tggctcgtcg cctcactggc gcgcgtctcg atcatgcgct cgagcgtctg
      721 cagtcgcgcg ccgagctgcg gccagagcgt cgtggcgtca ccgccggctc ccgtccggat
      781 cgacttctcg atggcaagca ggcggtccgc aatgagcgcg gtaccgcgct gtgcctcggc
      841 cgtgcgctga tcgacgagcc gctcggtccc cgcgaggcga tccgcgagcg cgcgctcgat
      901 cgcagtgagg cgatcgccga ggggggccgt atcaccgccg cgtgcgacgg cggcttcgat
      961 ggactgcagg cgctgcgcaa gcgctgacca gttccgcgcc ccctcgccga ggccgctgtg
     1021 cacggacttc tcgagtgtct ggacgcgctc ggcgaccatg acgaggtcct gcgccgcagc
     1081 agtccgctcg ttcaaggcca cctcgacgct ggcgaggcga tccgagagct gggtcatgtg
     1141 cccggcgagg ctcgctgccg gatcagggag gctcgcgagc ctggcagtga tgttcgactc
     1201 gagcgtctcg atgcgctcgg caaccatggt gggcacccgc tgtgcctcgg ccacgcgtgt
     1261 atcgacggcg cgctcgaccg actggaggcg ctcgctcgac agcgaccaga gccgcatcag
     1321 gtcggcgagc ttcacctcga tgccctgcat gcgctccgag agctgggtcg gcatgcgcgc
     1381 cggctctgga cgcgcgagcg cggtccgctc cagtgcctgc atgcgatcgc cgatgtcgcc
     1441 gcggagcgcc gcgacgtcgg tgcggtgcgc cgcgatatcg cgctgcaact cgcggatgag
     1501 gtcgccgatc gtcttgcggt cgatcgcgag ctcgccgtgc agcgagcgaa gcgacccctc
     1561 gacgccgtcg aggcgcgagc cgaacggatc ctggaccacg acggtctcca ggcgcggggc
     1621 gggccgcggc tcgggccggt actcgaggcg cggttcattg cgcagctcga tgcgcggctg
     1681 gcgatcacgg atccgcaccc agtcggagcg gtgccagtca ggtgcgtgaa cgcgcagcag
     1741 cgagattgcc gcgttgtcgc gcggatgcgt cagcagcacc cagagaatat cctcgaccga
     1801 cgtcacctcg ccgcggcggg ccgcggacga cgacgcgcgc cgcagcacgt cctcgatgtc
     1861 ctcggaccgg cgcggcggca ggcggtcgcc cgacatgccg atcggggcgt cattggcgat
     1921 gagggcggcg ctgtcgcggc ggagctgacc ttcacggatt ccgcggcgct cgaggatctg
     1981 ggccgcggca tcgacgcggg tgagcgcgtg cacgagatgg tcgaggcgga cctcgccggc
     2041 accgtgggcc agcgccacgt cgtaggcgtg attggcgacc gcgagcacga tgtcgtcgac
     2101 gagcagcggc tcgacgcgcc cgctcgaggg ggaacgcgac caacgccctg agctgccata
     2161 gccatcggcg tcggtcatcg catctcgatg atggtccgcg cgcggggggg tgcggagatc
     2221 gaggtcgttg cccttgtact gcatggctct gctcccaacc ggctcctgcg ccggcataac
     2281 ctgctcgagg ccgccgcggg gcgctcccac gaccgcggcc agctgacggt ccggacagcc
     2341 cggccgttgc cccttcgggg tgccgggccg ccggtgcatg acgggcgaag attatccgct
     2401 ttcggccgcg aattcgagtt tcggccgtgg cgatccctcg cgatggtcaa tttcccgact
     2461 gacgcctagt tggcgcaacc gggacttcgc cactctgcaa cctctacgta cgcatagtta
     2521 aagaagcgtc tgtttttgtg gcaaaatgag atctggcccc tcgtggcgcg ggttgccgat
     2581 gcgccggtcg atttcggtga tcgtcagcag gtcctcggga gccggcacga ggaggtcggc
     2641 cacctcagcg ggcgggactt tgcggcaatc gagccagcgc tcgaaggcct cgggcgcgag
     2701 gatcacgggc atgcggtcat gaatgcgacc gaccgtggca ttggcagccg tggtcaggat
     2761 tnnnnnnnnn ncgtagtggt gacgatgcag gcggtttcga gttcgctgcc gtcggcgccg
     2821 agccaatgct cgtggagacc ggcgaaggcg aggatacccc cgtcgtggcg ggcgagaaga
     2881 tggggctgct tgtggccggc ggggcccgtc cactcgtaga acccgttcgc ggggatgagg
     2941 cagcggcgat ggcgcatcga accgcggaag gatggctttg ccgcggccgt ttcggatcgc
     3001 gcattgatga gcgtcgagaa ctcccgcggg tccttgaccc agctcgggat cagcccccag
     3061 cgcacgaggg caagctcgcg ggcgacattg tcggccgaga gccgcacgat cgccaccggc
     3121 tgggacggcg caatgttgta gcgcggcgga aactcggcgt ccgtgcggta gccgaagtag
     3181 gcgcgcaccg cctcgtgcgg cgaggtcagg ttgtagcgcg agcacatggc cggacctacc
     3241 gatcggggag tacgcgcagc gtcgggcgct tcggcagggt cgcccgcgag atgacgatcg
     3301 tcggtcgtgc ctcgatttct acctcgaact cggcgcgggc gcgcgtcagg aaccgctcga
     3361 gcgtgtacgg cgaaaaatag tgcatcggca gcatcaggcg cgctttcagc gtcttcaagg
     3421 tctccatcat gccgtcgagg tccatcgtga agctgccgtc gacgggtacg agcacgatgt
     3481 cgagctggcc gatctgggcg agctgctgcg tcgtcagcgt gtgatggagg tggccgagat
     3541 gcccgatgca aaggtcagcc acctcgaaga tgaagatcga gttgccgaac tcgtaggtct
     3601 cgccgccgta gcggatgttg gtcgcaacgt tgcgcacgtg catgtcggag actgtgacgt
     3661 cgtgggccgc agcgccgccc ttcgggtccc agccgcgcag gacgtgcttg atgcgcggat
     3721 cggggacgtc ggtgtagtgg gtgttgtgcg cccggttcat ggtgacgacg tcgggcagct
     3781 cggccggccg cacatagtcg ttgtagtctg tcgccgcgcg aatgccggca gcactctcga
     3841 tcacgaacgt ggagtggccg acataggtga tgcgcacctc cgtccgcttg aggttcacct
     3901 cgcgaaagct cgtacgaatg atcgcgggcg tcccacctct ccccggcgcc tcggccatgc
     3961 caatgcaccg ttcggcggca agcgcaacgt cggccgacag cgcgcaactt gcagccgcca
     4021 gcagggcaac gaccattcgg agcagaatac gcatgctgcc tccgcaacaa ttccgagttc
     4081 tggcggccat agtgtccgtg cgagcgccga tcggcaagca agcgccgcga cgccggcgcc
     4141 gatcgatcac ttcttgagcg cctcgcgctc ctggtggcgg gcgatatgga agcgctggaa
     4201 attcgggtcc tgctcgtaga ggcgcagccg cacccatacg aacatgtcgt aaaaagtgcc
     4261 ggcgcccatg ccggccggca ttcgctcact cgcctcgagc acgcgcccga acttgccctc
     4321 gagaccggtg aggtcgtgct tcaggaagat gatcgtcggc gtgaagatga cgccccagcg
     4381 ctcggcgagc ttcttctctg tcattttgcg gccgtcgaag tcggtcacct cgcgcgcgcc
     4441 gaagagatca agctggatga cgtcgaagtg cgtgcgcaca taatcgttga tgtagcgctg
     4501 cgccagcacc tcggtgtgca tcttcacgca gtaggggcag ccgttctgct cgaagacgat
     4561 ggcgaagcgc ttgccggcct tgcgcgcctg ctcgtagtca tcgcggagat cgaggaatga
     4621 gttcacgaac caggtctgcg tgtagaggct cccttccttg aggggagcga ccttcggctc
     4681 ggccgggccg atcggcggcg gcaacggctc ggccgcgagc gagagcgctc gcggcagggc
     4741 cagcagcgcc acgagcagca gtccgatcga tatcgcgcgg tgtcgcatcg catcctcctc
     4801 ggagcagtct cctcagcata gactcggcgc cctttgcccg ggaagatcgc gcttgcacgg
     4861 cagaggtgca tttcgactgt tgcgcaaccg aactgcgctt cattgcctga gttcagtgcc
     4921 ggcgacccct ggtcgcgcca tgtttgcggg tgcatgatgt ctggagcgag atcggagatc
     4981 gggagcggga catgtcccca agacttgcca ttgtcggcgc cggtgccctc ggcggtcatg
     5041 tcggcggcta tctcgccaag gctcgccgcg atgtaacgct gatcgatgcg tggcccgagc
     5101 atgtcgaggc gatgcgcgca aagggcctca cgctcgccgg cctcacggcg gacgagaact
     5161 tcacgacgaa ggtgcgcgcc ttgcacatca ccgacgtcca gggggaggcg aagcgcccct
     5221 tcgatgtcgt cttcatcgcg gtcaaatcct acgacacgct ctgggccgcc gcgatgatgc
     5281 tcgactacgt ggcacccggc ggcttcttcg tctcgctgca gaacggcatc aacgaggagc
     5341 ggctcgcaag cgtcgtcggc tggggtcgca ccctcggctg catcgcgagc actatctcgg
     5401 tcgagctcac ggggccggcg cagatccagc gcaatgtgat gaagggtggc gccaagcaca
     5461 ccgtgtttcg cgtcggcgag ccgcatgggc ggatcacggc ccgcgcaaag ctcgtaaccg
     5521 agctcgtctc ggcggccgac agcgccaagg nnnnnnnnnn nnnnnnnnnn nnnnngggtg
     5581 agcgctggtc gaaactgatc gtcaacgcga tgcgcaatcc ggtttcagcc gcgagcgggc
     5641 gcggcggcaa tgccaacgac cgcgatccgg tgacgcgacg ggcggcgatc cgcatcgccg
     5701 cggaggcgat caaggtgggc ctcgcccacg gctacgttct ggaggacgtc tacaagatgc
     5761 cgccggcgca gatcgtcgcc gcggccgacg gcgatactga gatgctcgcg aagacggatg
     5821 ccatgctcct cgagggtacg aaatcccgta acgacgatca gcggccttcg atggggcagg
     5881 acatccgcaa aggccggcgg acggagatcg accacatcaa cggcctcgtc gttgcaaagg
     5941 cgcgcgaggc cgggattgcc gtgccggcca acgaggcgat catcgcagcg gtgaagcgcg
     6001 tcgaacgcgg cgaggccaag ccgtcacccg aactcgtcgc cggaatctga ggaggcgcgc
     6061 atgcgcagta cacacctgat cgcagcactt ctcgcaggct tggcagtgct cgccggcgga
     6121 gctgggcagg cgcttgccga cgccatcgac ggcgactggt gccgcgatgg cgcgagcttt
     6181 cgcatccgcg gcccctcaat cgtcacgccg ggcggcaatg ccatcacagg tgactacgac
     6241 cggcatggct ttcgctacgt cgtgccgccg agcgagcggc atgcgggctc ggagatcaac
     6301 atgtcgctga tgaacgacga cttggtggtc gtgcgccgcc gcgacggcgc caccaagtcc
     6361 gacatggagc ccgagacctg gcggcgctgc aaggtcacga gctgaaccgg gcgcgctatc
     6421 tgcgcgccca gctctgcggc atcggctatc attcgacgat cgacatcagt cgaagaagtc
     6481 aaggaacgag cggcgtcgct cgccagggcc acggtcaccg tagccgccac gacgctcgcg
     6541 acgatcgtcg tcatcatcgt cgtcgcgcca atggccgcga tgctcgtcgc ggcgctccac
     6601 tggacggtca tcgcgcggtc gtgccggcat ggcgcccgag aggaagctgc gcggctccgg
     6661 ttcggtgacg atcgaggcga gcttctcgag ctcaccgcgg tcgagccaca cgccgcgaca
     6721 ttgggtgcag gtgtcgatca gaacgccatt cctggtgatc tcgcgcatag caccctgaca
     6781 gactggacaa ttgaggacgc tcatgaattg cctccatttc gagagctgat cggggcacgc
     6841 ccagaaaggc gattttcgga tcggcgaacg cggacgagac cgcgagagga catagacaag
     6901 ctccgttcga attctgcgac cttgcctctg gcccaagcga gccaggcaga aagtgcaagc
     6961 atcggtcacc tccatcgagt gcgcttcgcc gcttgggtta ggccacggcc gaaacccggc
     7021 ttgctgcagt ggagtcggat gtggcgacgg tggcggccat cgcaattgcc aaagggtcca
     7081 gccagctcat cggcattttc gatcgcgctt gtttccgtga gcggctgcgc cacttctggg
     7141 gcgtgacgtc tttgcggcac cgtgcacgca tgcgccgagc aacgcttcga tcagctcgca
     7201 cgcaggcgcg ccgccgcgtg ctcatcctga ggggcccgtg gaaattttcg acgccttgca
     7261 gactgcccag ttcctgggca agacgatctg gatgtggcta ttcttcctcg gcaccgtgtc
     7321 ggttctgctg atcttcgatc tgggcgtgct gcacaaggag cagcgcgaaa ttcccgtgag
     7381 cgagagcctc gtgctttcag cggtttacat cggccttggc atcgcgttcg gcgcgtgggt
     7441 gtggtgggcc ctcggcgcag cgtcgggcat ggaatatctc accgggttcg cggtcgagaa
     7501 agcgttggcg atggacaacg tgttcgtcat cgccatgatc ttcgctttct tcgcgatacc
     7561 gtctcgctac cagcatcgcg tgctgttctg gggcattctc ggtgtcatca tcctgcgcgc
     7621 gatcatgatc ggcatcggcg cgaccatcgt cagccagttc tcttgggtgc tctacgtttt
     7681 cgcactcttc ctgatcgcga ccggcgtcaa gatgctgatc gtctcgggtc atgggccgag
     7741 cgtcgaggac aatcacgtgc ttcgcttcat gcgccgccgc ttcaacgtca ccgaccagct
     7801 gcacggcgaa aagttcttcg tcactgcgat cgatcctgcg agtggcaagt ctgcgcgctt
     7861 catgacgccc ttgtttctgg cgctggtgct gatcgaggtc gccgacctca tcttcgctgt
     7921 cgacagcgta cctgcgatct tcgcgatcac gaccgatccg ttcatcgtct acacgtcgaa
     7981 catcttcgcg atcctgggtc tgagggcgct ctattttgcg ctcgccgcga tggtgcatcg
     8041 cttccagcac ttgaagtacg cgctcgctct tgtcctgatc ttcataggct cgaagatatt
     8101 catcgccgat cttctcggta tcgaaaagtt cccgcccgtg gtcgcgctcg tcgtgacgtt
     8161 cggaatactg gccgcaggcg tgctgttctc gatgatcaaa acccgcagcc cgcgcgccac
     8221 gccctgaaac tcgcaaaatg gagtccggca tcccatggcc atctcaatcg aagaccctca
     8281 cgccgacagc accggcccgc atgtcgacct tccgcctgcg gtgacccggg ccggtcatgc
     8341 gatcggcgct tgggtacgca tcatcctgct caccgtgatc ggcgccatga cgctggtcgc
     8401 gaccgtcgcc ggcctcatcg atattgccag ggccggcaag gtgacgatct ccgacgtgct
     8461 cttgatgttc atctacctcg agatctgggc gatgatcgtg atcgaggcga cgacgcggcg
     8521 gttgccggtc aacttcatca tctacatcgc catcacggtg ctcacgcgcc acctcgtcgg
     8581 cgtcgcgggc gacaaatcga cgagcgacat cggcttgctg atagatgcgg gtgctatcct
     8641 cgttctggca cccgccgccg tgctgctcga gctgcgcttc ttcgggcgtc agcagcgcgg
     8701 cgagccggcg gcgcgctgag gaggatgatc aggcgacgcg tgccatcggc gcgacccgac
     8761 ggtggtcgcg ccgatggaac catggccagc tcccgagata cagcttaccg cgacggcgcg
     8821 ctcgcataag gcagcgcgcc atagagggac gccgtcgggc cggccggctt tgcctcggcc
     8881 gggaatgcgg ctgcctcgac gagctgtggt ccttctcggg tgagctcgat gatggcgagc
     8941 gcccgctcgg agaggccgga gctcgtgagg cggaacgctc cctcagtgcc cggaaatcct
     9001 tgcgggcgca tgagatcgct cgccgtgagc cgccggccgc tcggcgccga ggcgatcacc
     9061 acggcggcac tgacggcatc gaatgtgagt gtcgcgagcc gcgggggcgc catcccgtag
     9121 gccttcgtgt agcggccgac aaaatcgcgc cagccggcga cgtctggggc agcgtaccag
     9181 gcgccgagcg aggtcggatg cgtcgcgccg gagaggcctt cccacgcgcc cgttcccatg
     9241 agcttgatgc gacgcgtgtc gacgccggcc gcaaacaggg cctgcgccat gcgttgcagc
     9301 accgcaggcc cgcccgggac cacgattgca tcgatcgggc cacctgcccg ctccgcctcg
     9361 tcgatctgct ggcgcagcgt cgcggcgcat gtcgtcgcgc cggcactcgt ccccgcatgg
     9421 cgcgagcgcg ccgcgatgac accgccatgc cgcatgacag cggcctcgat caacggctcg
     9481 acgtaacgcc cgaacgcgtc gtccggcgcg gcgaccgcga agcgcttgcg accttggcgt
     9541 gcggccacct cgaccagtcg cgagacatcc tgcgagggca ggaagctcag aaggtgcacg
     9601 cccttgccgg cgacgcgatg gtcgttcgaa aaggcaatga cgggaacccc gttgcgctcg
     9661 gcgaccggcc cgacggccgc gaccgagccc gagaacaccg gcccgaggat cagctcggca
     9721 ccggcacgga tggcatcctc ggccgccacg cgcgctccgt cgggcgtgcc ccggtcgtct
     9781 ttcacgagga gctcgatgcg cgcgccgtcc cgctcgaaga gcgcgagttc gccggcctgg
     9841 cggagctggc gtccgagcgc ctgctgcggg ccggagagcg gcagcagcaa cgccactttc
     9901 cgtacgggct cgctgccgta gccgtccttg cgtgtgatcg ccttcggatc gtcgggccgc
     9961 gatgcgaccg agcctgtctt caggccctgg tactcgggcg tcgcgctcgt cgatcccgag
    10021 gagcaggccg cgagcgacac ggcggtgacg ccgacggcgg caagcgtgca cgcaaagcgg
    10081 agcacgcgcg gaagtgctgg ggtcgacatt cgccctcgtc ccgttgcgaa tcctattggg
    10141 gcgggtacga cctgacgctc cccgcaaccg accgggacga gtcactttgc tccgtggtcg
    10201 acctcaccta ggccgacgac ggcaattccg gtaagtgctc gggcgccact gtgcacaaca
    10261 ttcgcggcga ggaggctcga tgagcgaagc ccattccaat cgtgcggcac tccaaggcac
    10321 tgttgccggc gagctcgacc gctggctcga cgcgtcacct gccgtggggc tgtcgctcat
    10381 cgccacgccg atcggcaatc tcggcgacat cactcttcga gcgctcgcgg cgctggcgac
    10441 ggccgacgtc atctatgccg aggatacacg gcgcacgctg acgctgctta atcactatgc
    10501 gatccgccgc cccgtgcgca gtcatcacga gcacagcagc gcgacagaat ttgctgccgt
    10561 gcttgccgac ctcgaagccg ggaaggccgt ggcgctcgtg acagatgccg gcatgccgct
    10621 catctccgat ccgggctttc cgctggtccg cgaggccatc gccaagggcc atcgcgtgac
    10681 ggtcctgccg ggaccatcag ccgtgctcgc cgcactggcg gtcgggggct tgccggcgga
    10741 tacattcctg ttcgccggct tcctgccgcc caggacggcc ggacgccgcg ccgccctgg
//
LOCUS       DAORNH010000140        10660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4632_length_10660_cov_2.459595, whole genome shotgun sequence.
ACCESSION   DAORNH010000140 DAORNH010000000
VERSION     DAORNH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4632_length_10660_cov_2.459595"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK264_14245"
     CDS             <1..218
                     /locus_tag="PK264_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRD77068.1"
                     /translation="AVAGWLASIVVGGLKYGLIGYIVAGLLGSVVGGWLLNALKIGIN
                     LGHPLVNTIITSAIGAIVVILIARMIG"
     gene            complement(235..537)
                     /locus_tag="PK264_14250"
     CDS             complement(235..537)
                     /locus_tag="PK264_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD77069.1"
                     /translation="MTTEASPTETVQNCTVTAFGLTDSEAAALDMLVHGRTLSEAGKA
                     LAIVRTTAKTHLQHIYSKTRLSRQTDLVALVLKHAPPVRIGVKDEKHSTEACSPFS"
     gene            complement(527..979)
                     /locus_tag="PK264_14255"
     CDS             complement(527..979)
                     /locus_tag="PK264_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77070.1"
                     /translation="MSEEQTATVKLLAPHFRRVVAISNILNLAHVKSTDFATAPDAIA
                     VGIVPVDDEDRVLHAKTLANLMMARGASILARHGRITCRKIAARAELMSAIRLEPRNR
                     SGFAGNGNCISLASQDEVASVAHADADERWNAKPILKLGCNGGGFRDH"
     gene            complement(983..1192)
                     /locus_tag="PK264_14260"
     CDS             complement(983..1192)
                     /locus_tag="PK264_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77071.1"
                     /translation="MFKQSLLQQGDDVEPLTLSRGPFDWETYLRSPMHLEWAKPQGIK
                     DATNALVLMSSGRLGSRALITSYEQ"
     gene            1750..3297
                     /locus_tag="PK264_14265"
     CDS             1750..3297
                     /locus_tag="PK264_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HRD77072.1"
                     /translation="MNGAESLVRTLLAGGVDVCFTNPGTSEMHFVAALDKVPGMRCVL
                     GLFEGVVTGAADGYYRMLNKPASTLLHLGPGLANGLANLHNAKKANSGIVNIVGEHAT
                     YHIAHDAPLTSDIEGVARPMSHWVKTSPNSTTIAADGALAIEAARQTPGQIATLILPA
                     DTAWGPAHAIAETRTPAPPSTVAPDAVKASARMLTSGNRATLLLGGAALTPACLELAG
                     RIAAKTGCGILTEGANTRLARGAGRVQVDRIPYVVENALKVLQAAGDLVLVGSRRPVA
                     FFAYPNKPSLLMPEGARSTKLAGLEDDIEGALRALADELGASKLAPARVAQANRPALP
                     TGPITPASIAQVLGAKIPENAIVVDESVTTGREFFPSTAGAPPHDWINNRGGSIGYAM
                     PVAVGAAIACPDRKVIAMIGDGSAMYTVQAIWTMVRESLDITMLIFANGTYNILRGEL
                     TNVGVRNPGPRAVDMLSLGRPDLDWVSLARGMGCEGARVKTSEQLAKAIDAGLGSGGP
                     YLIQVDL"
     gene            complement(3317..4552)
                     /locus_tag="PK264_14270"
     CDS             complement(3317..4552)
                     /locus_tag="PK264_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD77073.1"
                     /translation="MGQFLFRNLELLDPDWDEPRGGYEVLVEGDRIKEVSAKPIKAGK
                     ATVVDCGRRTLMPGLIDSHVHVMLSEVYIRQLENVPLTLMTARAIDLMRGMIDRGFTT
                     VRDTGGCDWGLKTAVEQGIVSGPRLFIAGRAIGPTGGHSDGRRRTDRGGGCFCCNALK
                     FTMEVSDGADEVRKSAREQMRQGADQVKIMMSGGVASPYDPLYSLQFSPAEVAAAVEE
                     AHAFGRYVCAHAYTPEAITRAAMGGVRTIEHGNLIDDASAKLMAEKGMFLVANLVAYY
                     AMRERAREFGMTGEMLEKNDLVIDGGLKSLEICKRAGVPVAYGSDLLGQLQVDQSREF
                     LLRSEVLSPIEIIRASTTVGAKLLRQEGKLGCLKPGAFADLIVVDGNPVKNLGLFQEQ
                     GRHIPAIMQGGKFYRNRLA"
     gene            complement(4561..5355)
                     /locus_tag="PK264_14275"
     CDS             complement(4561..5355)
                     /locus_tag="PK264_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD77074.1"
                     /translation="MQLGTTKMIARKDGGIGWMIFNQPEKRNAVSYEMWAAIPKIIAD
                     FEADPAVRVIVLTGAGDKAFVSGADISEFDKRRDREETIKVYDAASEAASAAIAEATK
                     PTISMIRGLCVGGGVAIMLNTDIRICGHDSYFAVPAARLGLGYRPSGIKKLVDVVGPA
                     FAKEIFFTANRFTAEDARIMGLVQRVVPAEGLEAYVREYAAKIADNAPLTIKAAKLAI
                     DACIKDAEKRDMSRVNDAVAACFASADYIEGRRAFMEKRRAGFKGR"
     gene            5465..5707
                     /gene="thiS"
                     /locus_tag="PK264_14280"
     CDS             5465..5707
                     /gene="thiS"
                     /locus_tag="PK264_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRD77075.1"
                     /translation="MQEKTTRSPPAAPATLAIFVNGEPTASHAGTLAELVASLGHGGA
                     RVATALNGDFVPEKRRPDTRLSPGDRVEIVSPRQGG"
     gene            5812..5887
                     /locus_tag="PK264_14285"
     tRNA            5812..5887
                     /locus_tag="PK264_14285"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5845..5847,aa:Phe,seq:gaa)
     gene            6012..7643
                     /locus_tag="PK264_14290"
     CDS             6012..7643
                     /locus_tag="PK264_14290"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HRD77076.1"
                     /translation="MAKTADGDIFDYVIVGAGSAGCALASRLSEDGRTRVALVEAGSR
                     DTDPWIHIPAGYFRTMFNPAVTWQYGSGPEPFCDNRIVAWPRGRVLGGSSAINGLLYV
                     RGQSQDYDMWRQLGNPGWAFKDVLPYFRRSEDQERGESELHGAGGPLGVSDVRMQNPL
                     CEAYIAACVEAGIPATRDFNGVSQEGAGYYQLTVRNGRRCSSAVAYLNPARRRHNLRI
                     ITDAEVQAITFAGRRANGIRFVAGGAEKTVTATREVILCAGAIGSPQLLEVSGIGAPA
                     VLKAAGVDVRHELPGVGENMQDHYQVRFVYEMNVSESLNDVWNKPLKQLKAGVEYATK
                     RTGILTIGAGVVGVFTQSRQGLDLPDIQFHMMPLSAERPGQGLHPFSGVTASVCVLRP
                     DARGHIHIRAPEFRVPPEIISNYLGTESDRAATLGGLKLARRIAAQPSFARLVKREVL
                     PGIEAASEDALMAFARAMGTTVFHPCGTCKMGQDGKSVVDERLRVRGIGGLRVVDASI
                     MPTLISGNTNAPSIMIAEKASDMIREDARTAAAAA"
     gene            7746..>8000
                     /locus_tag="PK264_14295"
     CDS             7746..>8000
                     /locus_tag="PK264_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77077.1"
                     /translation="MTPPLTIAPSIDLAARPREACFLSLDLKAAVGADGAFEGYASLF
                     DREDLGRDVVTRGAFRDSLAERGAAGVRMLVVPGSVRIKRE"
     assembly_gap    8001..8184
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8268..8426
                     /locus_tag="PK264_14300"
     CDS             8268..8426
                     /locus_tag="PK264_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77078.1"
                     /translation="MRDAGLTRSEARALMRSGFKGLAATQDAGPCPDQSRRLAATIRD
                     AARALAPR"
     gene            8444..9688
                     /locus_tag="PK264_14305"
     CDS             8444..9688
                     /locus_tag="PK264_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRD77079.1"
                     /translation="MTDTPHAPEIKSSDADVGAAFEDFMRSFEAFKDGNDQRLAEIER
                     RSAADPVTIEKLSRIERALDESRRVVDALALKAARPHLGGATAITGAALQHKSAFEHY
                     VRKGETGNLRSIEQKALSVGSDPDGGYLVPDETERTVMRALRTVSPIRAIATVRQVSG
                     AVYKKPFSISGAGAGWVGETAARTETTTPTLAELSFPTMELYAMPAATSAVLDDSAVS
                     IDEWLADEVRDAFAAQEGNAFVIGNGTNKPRGFLDYPKVDNATWSWGNVGFIATGAAG
                     AFPASNPTDKLIDLLYAVKSGYRANASFVMNRATQSAIRKFKDADGNYIWQPSPRAGE
                     PPILLGHPVAECEDMPTIATDSTSVAFGDFRRGYLIVDRIGLRVLRDPFSSKPYVLFY
                     TTKRVGGGIQDFDAIKLLKFAA"
     gene            9792..10367
                     /locus_tag="PK264_14310"
     CDS             9792..10367
                     /locus_tag="PK264_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HRD77080.1"
                     /translation="MSLVLASGPATEPVSVAEAKAHLRVDHTAEDPFIGSLIVTSRLH
                     IEAALGLALITQSWSYFIDRFPRSGELVLPMRPVQSVSAVRIWQSDDTSAAAPASAYL
                     LDGTALPPRLVWCNAPFPTAGRRAKGIEVAMTVGYGPAPADVPAPISHALLLLIAHWY
                     EHREPIEVGEALAPIPVAVSELLAPYRARHL"
     gene            10364..>10660
                     /locus_tag="PK264_14315"
     CDS             10364..>10660
                     /locus_tag="PK264_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HRD77081.1"
                     /translation="MSAFAIRLLRHRLDIQAPVRTPDGGGGATETWSSTATVWAAIRP
                     TGAGEPIVADAHRGEVSHEIWIRFRNDVLPGMRFAQGSRVFEIVGVIDVGERHRR"
BASE COUNT         1719 a         3817 c         3178 g         1762 t
ORIGIN      
        1 gcgccgttgc cggctggctc gcgagcattg tcgtcggtgg actgaagtac ggcctgatcg
       61 gctacatcgt tgccggactg ctcggcagcg tggtcggcgg ctggctgttg aacgcgttga
      121 aaataggcat caatctcggc cacccgctcg tcaacacgat catcacgtcg gccatcggcg
      181 cgatcgtggt catcctgata gcgagaatga tcgggtagcg tcagggttgt cagctcagga
      241 gaagggcgag caagcctcgg tggagtgctt ttcgtccttc accccgatgc gaacaggcgg
      301 ggcatgcttc agcaccaggg ccaccagatc ggtctggcga gagagccggg tcttggagta
      361 gatgtgctga agatgtgtct tggctgtcgt gcgtacaatt gccagagctt tgccggcctc
      421 cgatagtgtg cgcccatgca cgagcatatc gagcgcagca gcctccgaat cggtcagccc
      481 gaatgcagta accgtgcagt tttgcaccgt ctcggtcgga ctggcctcag tggtcacgaa
      541 agccgccgcc gttgcacccg agtttgagta tcggtttcgc attccaccgc tcatcggcat
      601 cagcatgtgc caccgaagcc acctcgtctt gcgaagcgag cgagatgcag tttccgtttc
      661 cagcgaaccc cgaacgattt cgtggctcca gtcgaatcgc gctcatcaac tcggctcgtg
      721 ctgcaatctt gcggcatgtt atgcgaccgt gccgagctag aattgacgcg ccacgcgcca
      781 tcatgaggtt tgccagggtc ttcgcgtgca gtactcggtc ttcgtcgtcg actggcacga
      841 tgccgacagc gattgcatca ggtgccgtgg cgaagtccgt ggacttcacg tgcgccagat
      901 tgaggatgtt gctgatcgca accacccttc ggaaatgtgg cgccagcagc ttgaccgtcg
      961 cagtttgctc ctcgctcacg gctcactgct cgtagctcgt aatcagggca cgtgagccga
     1021 gacggccaga cgacatcaga acaagcgcgt tcgtcgcatc cttgatgccc tgcggcttgg
     1081 cccattccag atgcattggc gagcgaaggt aggtctccca gtcgaatggc cccctcgaca
     1141 gggttagcgg ctccacatcg tcaccctgct gcagaagact ctgcttgaac attccggccg
     1201 atgcctcggc cagcggtcgg accgaggcca tgtcgagaaa gcgtcgacca atgttccagt
     1261 gcgcaagatg tcattggcgg agaaagtcga ggtcgatcat caggagagcg ctagcagtga
     1321 agcctagtcg ctggcagagc atccccgaga cccatggcca tctgtcgggg tcgagagcgc
     1381 gatctagagt gcccccaaca gcatggagag ccgtgccagt tcggaggcca tacaatcctc
     1441 cttctgctcg cccgagctag tcagtgtttt gtgggtcgtg aacgctcaaa ttccaatgtg
     1501 cacacgcatc gacgggctcg ctaacgtcgg cctcaggctc ccaacattcg tcgattttgg
     1561 gcaatgaaca tccctccagg tcaataagaa tcttcgtgcc ttcggaagcc acttgcaggt
     1621 ccgatccacc cttgccgcac tgatcgcatt gaattgccgg ggcgggcggt ctagcgtctg
     1681 ctcaatggcc ggggtcgctt gtcgctccgg ccttcgccac gccatgggag cccgccgatg
     1741 accgagccga tgaacggcgc cgagagcctt gtccgcacgt tgcttgccgg cggcgtcgac
     1801 gtctgcttca cgaacccggg cacctccgag atgcacttcg tcgcggcgct cgacaaggtg
     1861 ccgggcatgc gctgcgtgct cggcctgttc gagggcgtcg taacgggtgc cgccgacggc
     1921 tactaccgga tgctcaacaa gcctgcttcc acgctgctcc atctcggacc cggcctcgcc
     1981 aacggccttg caaacctgca caacgccaag aaggcgaact ccggcatcgt caacatcgtc
     2041 ggcgagcacg cgacctatca catcgcccac gatgcgcccc tcacctccga catcgaaggt
     2101 gtcgcgcggc ccatgtcgca ctgggtcaag acctcgccca actccaccac gatcgccgcc
     2161 gatggagcac tcgccatcga agccgcgcgc caaacgcccg gccagatcgc cacactgata
     2221 ctgcccgccg acacggcttg gggtcccgcc cacgcgattg ccgagacgcg cacccccgcc
     2281 ccaccgagca ccgtcgcccc tgatgccgtg aaggcttcag cgcgcatgct gacaagtggc
     2341 aatcgcgcga cgcttctcct cggcggcgcg gcattgacac cagcctgcct ggagctcgcc
     2401 ggacgcatcg ccgccaagac gggctgcggc attctcacag agggcgccaa tacgcgcctt
     2461 gcccgcggcg ccggacgcgt ccaggtcgat cgcattcctt atgtcgtcga aaacgcgttg
     2521 aaggtgctgc aggccgcggg tgatctcgtg ctcgtcggct cgcgccgccc cgtcgccttc
     2581 ttcgcctatc cgaacaagcc gagcctgctg atgcccgaag gcgccaggtc gacaaagctc
     2641 gccggcctcg aggacgatat cgaaggcgcg ctccgggcgc tcgccgatga gcttggcgca
     2701 tcgaagctcg ccccggctcg agtagcgcaa gctaaccgtc ctgccctccc gacgggcccg
     2761 atcacgccgg cctccatcgc ccaggtgctc ggcgccaaga tccccgagaa cgccatcgtc
     2821 gtcgatgaaa gcgtcaccac cggccgcgag ttcttcccct cgaccgcggg tgccccaccg
     2881 cacgattgga tcaacaatcg cggcggctcg atcggctacg ccatgcccgt cgctgtcggt
     2941 gccgccatcg cctgccctga ccgcaaggtc atcgccatga tcggcgacgg cagcgccatg
     3001 tacacggtgc aggcgatctg gacgatggtg cgcgagagcc tcgacatcac catgctgatc
     3061 ttcgccaacg gcacttacaa tatcctacgc ggcgagctca ccaacgtcgg cgtccgcaac
     3121 cccggaccgc gcgctgtcga catgctctcg ctcggccgcc ccgacctcga ctgggtgtcg
     3181 ctcgcccgcg gcatgggctg cgagggcgcg cgcgtgaaaa cctccgagca actcgccaaa
     3241 gcgatcgacg ccggcctcgg ctccggcggc ccctacctca tccaggtcga cctctgagtc
     3301 gggcggcggc gcctcctcag gcgagcctgt tccgatagaa cttcccgccc tgcatgatgg
     3361 cgggaatgtg ccgcccctgc tcctggaaga gcccgaggtt cttcacaggg ttgccgtcga
     3421 cgacgatgag gtcggcgaag gcgcccggtt tcaggcagcc gagcttccct tcctggcgaa
     3481 ggagcttggc gccgaccgtc gtcgacgcgc ggatgatctc gatcggcgac agcacctccg
     3541 agcgcagcag gaactcgcgc gactggtcca cctgcaattg cccgaggaga tcgcttccgt
     3601 aggcgacagg cacaccggcc cgcttgcaga tctcgagcga tttcaagccg ccgtcgatga
     3661 cgaggtcgtt cttctcgagc atctcgcccg tcatgccgaa ctcgcgcgcc cgctcgcgca
     3721 tcgcgtagta ggcgacgaga ttggcaacga ggaacatgcc cttctcggcc atcagcttgg
     3781 ccgaggcatc gtcgatcagg ttgccgtgct cgatggtgcg cacgccaccc atcgccgccc
     3841 gcgtgatcgc ctcgggcgtg taggcatgag cgcacacgta acgcccgaag gcgtgcgcct
     3901 cctccaccgc tgcagcgacc tccgcgggcg agaactgcag gctgtagagc gggtcgtagg
     3961 gcgaggcgac gccgcccgac atcataatct tcacctggtc ggcgccctgg cgcatctgct
     4021 cgcgcgccga cttcctcacc tcgtccgccc cgtccgacac ctccatcgtg aatttcaagg
     4081 cgttgcagca gaagcatccg ccgccccggt ccgtgcgccg ccgcccgtcg ctgtggccgc
     4141 ccgtcggccc gatcgcgcgc cccgcgatga aaagcctcgg acccgagacg atgccctgct
     4201 cgaccgcggt cttgaggccc cagtcgcagc cgcccgtatc gcgcaccgtc gtgaagccgc
     4261 gatcgatcat gccgcgcatc aggtcgatcg cccgcgccgt catcagggtg agcggcacgt
     4321 tctcgagctg gcggatgtag acctcggaga gcatcacgtg cacgtggctg tcgatgaggc
     4381 ccggcatcag cgtgcgcctg ccgcaatcga cgacggttgc cttgcccgcc ttgatcggct
     4441 ttgccgacac ctccttgatg cggtcgccct cgaccagcac ctcatacccg ccgcgcggct
     4501 cgtcccagtc cggatcgagc agctcgaggt tcctgaacag aaactgaccc atggaccgcc
     4561 ttacctccct ttgaatcccg cccgccgctt ctccatgaag gcgcggcggc cctcgatgta
     4621 gtcggcactc gcgaagcagg cggcaacggc atcgttcaca cgggacatgt cgcgcttctc
     4681 ggcgtccttg atgcaggcat cgatggcgag cttcgctgcc ttgatcgtga gcggcgcgtt
     4741 gtcggcgatc ttcgccgcgt actcgcgcac gtaggcctca agcccctcgg ccggcaccac
     4801 gcgctgcacg agccccatga tgcgcgcgtc ctccgccgtg aagcggttcg ccgtgaagaa
     4861 gatctccttg gcgaacgcag gtcccaccac gtcgacgagc ttcttgatgc ccgaaggccg
     4921 gtagccgagg cccaagcgcg ccgccggcac cgcgaaatag ctgtcgtggc cgcagatccg
     4981 gatatccgtg ttgagcatga tcgcgacacc gccgccgacg cagagcccgc ggatcatcga
     5041 gatcgtcggc ttcgtcgcct ccgcaatcgc ggccgaggcc gcctcgctcg ccgcgtcgta
     5101 gaccttgatc gtctcctcgc ggtcgcgccg cttgtcgaat tcggagatgt cggcgcccga
     5161 gacgaacgcc ttgtcacccg cccccgtcag cacgatcacg cgcaccgccg gatcggcctc
     5221 gaagtcggca atgatcttcg ggatcgcggc ccacatctcg taggacacgg cattccgctt
     5281 ctccggctga ttgaagatca tccagccgat gccgccatcc ttgcgggcaa tcatcttggt
     5341 cgtgccgagt tgcatggcgc tctccgttgg ccgtaccggg gccacgcgtg gcctccgctc
     5401 gaaggccatg ctagacggaa gtgagcgctc ccgactacac gatcgaccga aattcatgcg
     5461 ggccatgcaa gagaagacga ccagatcacc ccccgccgcc ccggccacgc tcgccatctt
     5521 cgtcaacggc gagccgacgg cgagccatgc gggaacactc gctgagctcg tcgcgagtct
     5581 cggccacggc ggagcgcggg tggcaacggc gctcaacggc gatttcgtcc cggagaagcg
     5641 tcgccccgac acgcgcttga gccccggcga ccgcgtcgag atcgtctcgc cgcgccaggg
     5701 cggctgagcc gatccggatg caaccggcac tcccacattc gcgctggaca cacctcgtgc
     5761 cggaccttat ataacccgag cttcgcggcc ggccgtgcct cgccgcgggc agcccaggta
     5821 gctcagctgg tagagcacag cactgaaaat gctggtgtcg gcagttcaac tctgcccctg
     5881 ggcaccacct gcctcccctt cgatcggcac tctcgccaca tttggcgttg gcatcgctcg
     5941 cccgcccccc taacatttgc gcgatccaat cggcgcatgc gtggcccgcg acggccggca
     6001 aaggggatgt catggcgaaa actgctgacg gcgatatttt cgactacgtg atcgtcggcg
     6061 cgggctccgc cggctgtgcg ctcgcaagcc gcctgtcgga ggatggccgc acccgcgtcg
     6121 cgctcgtcga agccggcagc cgcgacaccg atccctggat ccacatcccg gccggctact
     6181 tccgcacaat gttcaacccg gctgtcacct ggcagtacgg ctccggcccc gagccattct
     6241 gcgacaatcg cattgtcgcc tggccgcgcg gccgcgtcct cggcggctcg agcgccatca
     6301 acgggctcct ctacgtccgc ggccagtcgc aggactacga catgtggcgc cagctcggta
     6361 atcctggctg ggccttcaag gacgtgctcc cctatttcag gcgctccgag gaccaggagc
     6421 gcggcgagag cgagctgcat ggtgccggcg gcccgctcgg cgtctcggac gtgcgcatgc
     6481 agaacccgct ctgcgaggcc tacatcgccg cctgtgtcga ggccggcatc ccggcaacgc
     6541 gcgacttcaa cggcgtctcg caggaggggg ccggctacta tcagctgacc gtccgcaacg
     6601 gccgccgctg ctcctccgcc gtcgcgtatc tcaatcctgc ccgccgccgg cacaacctgc
     6661 gcatcatcac cgatgccgag gtacaggcga tcaccttcgc cggccggcgc gccaacggca
     6721 tccggttcgt tgcaggcggc gcggaaaaaa cggtcaccgc cacacgcgag gtgatcctat
     6781 gcgccggcgc cataggttcg ccgcaactcc tcgaggtctc cggtatcggt gcgcccgccg
     6841 ttctcaaggc cgccggcgtc gatgtgcgcc atgaacttcc gggcgttggc gagaacatgc
     6901 aggaccacta ccaggtacgc tttgtctacg agatgaacgt ctccgaaagc ttgaacgacg
     6961 tctggaacaa gccgctgaag cagctgaagg ccggcgtcga atacgcaacg aagcgcaccg
     7021 gcatcctcac catcggcgcg ggcgtcgtcg gcgtcttcac gcagtcgcgc caaggcctcg
     7081 atctacccga catccagttc cacatgatgc cgctctccgc cgagcggcca gggcagggcc
     7141 tccacccctt ctcgggcgtg acagcctccg tctgcgtgct gcgtcccgat gcccgcggcc
     7201 acatccacat ccgcgcgccc gagttccgcg tgccgcccga gatcatctcg aactacctcg
     7261 gcaccgagag cgaccgcgcc gcaacgctcg gcgggctgaa gctcgcgcgg cgcatcgcgg
     7321 cgcagccctc gttcgcccgt ctcgtcaagc gcgaggtgct ccccggcatc gaggccgcct
     7381 ccgaggacgc cctgatggcc ttcgcccgcg ccatggggac gacagtgttc catccctgcg
     7441 gcacctgcaa gatgggccag gacggcaagt ccgtcgtcga cgagcggctg cgcgtccgcg
     7501 gcatcggcgg cctgcgcgtc gtcgacgcct cgatcatgcc gacgctcatc tcggggaaca
     7561 ccaacgcgcc ttccatcatg atcgccgaga aggcatccga catgatccgc gaggatgccc
     7621 ggactgctgc ggccgccgcc tgacaccggt cgcgcgcgga ctgaccgtct gccgataatc
     7681 cccgcttctg ccgcttgagg cattctcagc gcatgcaatc gctgcgcttc gggactgcct
     7741 caggcatgac acctcccctc accatcgcac cgtcaatcga tctcgccgca cgccctcgcg
     7801 aggcgtgctt cctctcgctc gacctcaagg ccgccgtcgg agcagacggt gccttcgaag
     7861 gctacgcgag cctcttcgat cgcgaggacc tcggccgcga cgtcgtcacg cgcggcgcct
     7921 tccgtgacag cctcgccgag cggggtgcgg ccggcgtgcg catgctcgtc gtgccgggtt
     7981 cggtgcggat caagcgcgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnagcgag tacaagcgac ggcaggctcc ggtcggcatc
     8221 cgtgtcacgc agcgcggtcg tcggagagag agttcgagcg ctggctcatg cgggatgctg
     8281 ggctcacgcg ctccgaggcc cgcgcactca tgcgctcggg cttcaaaggg ctcgccgcca
     8341 cgcaggacgc tggcccgtgc cccgaccaga gccgccggct cgccgccacc attcgtgacg
     8401 ctgcccgcgc gcttgccccc cgttgatcca acccaggacc accatgacag acactcccca
     8461 cgcccccgag atcaagtcgt ccgacgccga cgtcggcgcc gccttcgagg acttcatgcg
     8521 ctctttcgag gccttcaagg acggcaatga tcagcgtctc gccgagatcg agcgccgctc
     8581 cgcggccgac cccgtcacga tcgagaagct ctcccgcatc gaacgcgcgc tcgacgagag
     8641 ccgccgcgtc gtcgacgccc tcgcgctcaa ggccgcccgc ccgcatctcg gcggcgcaac
     8701 ggccatcact ggcgcagccc tccagcacaa gtccgccttc gagcactacg tccgcaaggg
     8761 tgagaccggc aacctccgct cgatcgagca gaaggcgctc tccgtcggct ccgatcccga
     8821 cggcggctac ctcgtccccg atgagaccga gcgcaccgtg atgcgcgcgc tgcgcaccgt
     8881 ctcgccgatc cgcgccattg cgactgtccg gcaggtctcc ggcgccgtct acaagaagcc
     8941 gttctcgatc tccggtgccg gcgccggctg ggtcggcgag accgcggccc gcaccgagac
     9001 cacgacgccg acgctcgccg agctctcctt cccgacgatg gagctctacg cgatgccggc
     9061 cgcgacctcg gccgtcctcg acgacagcgc cgtctcgatc gacgagtggc tcgccgacga
     9121 agtccgagac gccttcgccg cccaggaagg caacgcattc gtcatcggca atggtaccaa
     9181 caagccgcgc ggcttcctcg actatcctaa ggtcgacaat gcgacctggt cgtggggcaa
     9241 tgtcggcttc atcgccaccg gtgccgccgg cgccttcccg gcctccaacc cgaccgacaa
     9301 gctgatcgat ctcctctacg cagtgaagtc gggctatcgc gccaacgcct ccttcgtcat
     9361 gaaccgcgcg acgcagtccg cgatccgcaa gttcaaggat gccgacggca actacatctg
     9421 gcagccgagc ccgcgggcgg gtgagccgcc gatactgctc ggccacccgg tcgccgagtg
     9481 cgaggacatg ccgacaatcg ccaccgacag cacctcggtc gccttcggcg atttccgccg
     9541 cggctacctg attgtcgacc ggatcggcct ccgcgtcctc cgcgatccct tctcgtcgaa
     9601 gccgtacgtg ctcttctaca cgacgaagcg cgtcggcggc ggcatccagg acttcgatgc
     9661 gatcaagctt ctgaaattcg ccgcctgatc gcgtcgagcc cggccgggtg gtccctcccg
     9721 ccacccggcc ggcacttgcg gtggagccag tccccggtgc cgccgccacc acaacccccg
     9781 aggaattccc gatgtcactc gtgcttgcga gtggcccggc aaccgagccc gtttcggtcg
     9841 ccgaggccaa ggcccacctg cgcgtcgatc acaccgccga agacccgttc atcggcagcc
     9901 tcatcgtcac ctcgagattg cacatcgagg ccgcgctcgg cctcgcgctc atcacccaga
     9961 gctggagcta cttcatcgat cgcttcccgc gctcgggcga gctcgttctg ccgatgcgcc
    10021 cggtccagtc ggtgagcgcc gtccgcatct ggcagtccga cgatacctcg gccgcagcgc
    10081 cggcctcggc ctacctcctc gacggcaccg cgctgccgcc acgtctcgtc tggtgcaacg
    10141 cgccctttcc gaccgccggc cgccgcgcca agggcatcga ggtcgccatg accgtcggct
    10201 acgggccggc gcccgccgac gtgcctgcgc ccattagcca cgccctcctg ctcctgattg
    10261 cccactggta cgagcaccgc gagccgatcg aggtcggcga ggcgctcgcg ccgatcccgg
    10321 tcgccgtctc cgagctactc gccccctatc gcgcgaggca cttgtgagcg ccttcgccat
    10381 ccgcctcctc cgccaccgcc tcgacatcca agcgccggtc cgcacgcccg acggcggcgg
    10441 cggggcaacc gagacctggt cgtcgacggc gacagtgtgg gcggcgatcc gtccaaccgg
    10501 cgccggcgag cccatcgttg ccgatgccca tcgcggggag gtttcgcacg agatctggat
    10561 ccgcttccgc aatgatgtgc tgccaggcat gcgcttcgcc caagggagcc gcgtcttcga
    10621 gatcgtcggc gtcatcgatg tcggcgagcg tcaccggcgg
//
LOCUS       DAORNH010000141        10656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4633_length_10656_cov_2.766437, whole genome shotgun sequence.
ACCESSION   DAORNH010000141 DAORNH010000000
VERSION     DAORNH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_10656_cov_2.766437"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PK264_14320"
     CDS             complement(<1..817)
                     /locus_tag="PK264_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRD77082.1"
                     /translation="MAAAEATPGSEAGGGAKPVGKGSHVYLIDGSGYIFRAFHALPPL
                     TRPSDGLPVGAVHGFCQMLWKLIQEAKGKDGPSHIAVIFDTSRDTFRSKIYEAYKANR
                     PPPPEELVPQFPLIREAVTAFNVTLVEKDGYEADDLIATYARETVAAGGDVTIVSSDK
                     DLMQLVGPNVAMLDTMKNRRIGHAEVIEKFGVPPEKVVDVQSLAGDSVDNVPGVPGIG
                     IKTAAELITQYGDLDTLLAKAGEIKQPKRRERLIEFADQARVSRDLVRLKDDVP"
     gene            1033..1539
                     /locus_tag="PK264_14325"
     CDS             1033..1539
                     /locus_tag="PK264_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HRD77083.1"
                     /translation="MRAILSRPNALLLSAVAGGGALAMSLSVVPGAAQSPGVVSYNKK
                     AAYDDVKFELNNAIVGRGLTIDYNGQLGRMLERTGADVGSTKPIYKNAEYFAFCSAKL
                     SRAAMEADPANIAGCPFIVFIYETAAAPGTVHVGYRRQPSHGAAASVKAHADIDALLD
                     GIAKAAVK"
     gene            complement(1567..2433)
                     /locus_tag="PK264_14330"
     CDS             complement(1567..2433)
                     /locus_tag="PK264_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77084.1"
                     /translation="MKMTPTAYAFNAIGALVAIGTLAVIVRSTLMPATEAPCGERYAE
                     GVLFAFEGASGAPFTTEDFEARLAGDDWGIKENFRVVPAKGLEHKHAMRIALPKGSLD
                     PAEKSALRGGVAFRWEPRTMTPKAAACLGYRVRLPADFDFAGGGRLPGLMGGERQDSA
                     AVPEKARAFTARPMWRAAGVVELNLETPEGLDYGPARVSMPKGQWVQIEQEIVLNTPG
                     KRDGILRLWVDGRLASERRNVAFRAHASHLVTGVAAEVTSAARGLLNAPPKEQAIEIS
                     PFELRWGPIEGN"
     gene            complement(2446..3399)
                     /locus_tag="PK264_14335"
     CDS             complement(2446..3399)
                     /locus_tag="PK264_14335"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRD77085.1"
                     /translation="MERLPGTSRSLPRLDIRGPVLLGAAVLAIGVGGALAAAAVAPID
                     KGASLSGTLVVETRTRAVQHDRGGVVAEVHVKEGAEIDAGALIVTLDTSALGEQIAAL
                     RAQQQAASRQMELVRQEVQAIGELVAKQLIQRSRLLALERQLSELEKEAASLIARISI
                     AEQDARKAQVRAPVSGRLLMLAVHGTGGVLTPGQTVAEIVPKDDRLVIEGRLSLALID
                     LVKPGMPAKVWLTGTTWREQRPLAARVAWISPDSVEDKRSGVAFFSARIELADPKSEL
                     VKEQRIHPGQRAEILVVTGARTLLDQLLDPIMRNVNRAFRS"
     gene            complement(3409..5139)
                     /locus_tag="PK264_14340"
     CDS             complement(3409..5139)
                     /locus_tag="PK264_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77086.1"
                     /translation="MRTSRQILKQARRALVAALIFSGGINVLMLAMPIYTLQVFETVV
                     PSGSIETLILLTLMIGGATLALALIEIVRDRILLRAGLWLDHVLGSFLIETGLRSGSS
                     AIDLKHDARALQGLRTFISSGAIGTLFDAPWVPIFLVTLALLHPLLGVAGAVAAVLLF
                     GTGLLQCLLAARVAPEAQKGQERAERWWAMLAERGQIAGALGLATGATSQWEAHNRTH
                     VANAYSLGKRTSVLKAVARAIRLAAQVSIYALGAFLVIRGDVTPGVLVAAAILLGRAL
                     APLEQSVTSFRAAGSAWTAYKRLRAIADTVRPPGIAEAGHSPEGRLTIAGVTYYYPTR
                     RTPALRNLTFELEPSMCLAIVGPNGAGKSSLSGLIAGALLPTQGAVQLDGVAVGRWQR
                     SDTLPPIGYMPEPILIEGTVHDNIARFSDASHMGVARAAMRAGVHDMLSALPNGYDTA
                     VGAGGEALSLRERRAVALARALHGEPRIVVLDEPELGLDAVGVRRLRNVLAGLKAEGV
                     GIVIATQDQRLLSLADRMAILANGAVEDYGPADQVAKRLMAGKPVAAVQSHPHAAPTE
                     AAPPRLQAAH"
     gene            5316..6119
                     /locus_tag="PK264_14345"
     CDS             5316..6119
                     /locus_tag="PK264_14345"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD77087.1"
                     /translation="MNRLDNKVALVSGAARGIGAATARLMASVGARVVIGDILEERGR
                     ETVKEIQSEGGKAAFARLDVTSEASWNGAIGHATREFGGLDILVNNAGIFLGKDFEEA
                     SLDDWHRLVAINMTGVFLGTKLAVPALKESGARSKHGSAIVNLASIAGLVGSQLDPLY
                     SMTKGGVTLFTKSTALAFGRKRYPIRVNSIHPGVIDTDMGAQTFVSRARNAGTNDVSS
                     ARAASTSMHPIGRLGTADDIARGIVFLASDDSAFMTGSGMVVDGGITAQ"
     gene            6249..6998
                     /locus_tag="PK264_14350"
     CDS             6249..6998
                     /locus_tag="PK264_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77088.1"
                     /translation="MTDQDHALDAAKVRRFGIMAAIAIALISAAGIAWFTIGGGKAFV
                     TGIVTALDRQVSANFMPAVATFLVIAFILQLFVLPTGTIAMLTGGFLFGAPLAAALYY
                     GAQILAAPVVYGAVRLGFGSFADEKLDGFVKRYLPARFSSIVEIARSEGLLAAISLRL
                     APVITSPVVPILAAATGIKLAALIAGTILVGWVRPLFWASVGATAHSLAEVTSSSEFL
                     SKVNLTPLLLAFAAAAAIFVLRLVLRLRTDK"
     gene            7026..7577
                     /locus_tag="PK264_14355"
     CDS             7026..7577
                     /locus_tag="PK264_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HRD77089.1"
                     /translation="MSLETFSNRTTTRTPAYAAGAMVLLAALAVIIAALAFEHIGGHV
                     PCELCLRQRWPYYVGIPLAFLALVLLSAGQAKAAALLFLAAALGFLLNAGFGVHQVGV
                     EWGYWPGPATCSGNAMQPLGLPAGGDLLKSLQTVRIARCDAPTWHFLGLSFAGWNVPI
                     SLALAVGALKAAYDALGRSAVRL"
     gene            7642..8754
                     /locus_tag="PK264_14360"
     CDS             7642..8754
                     /locus_tag="PK264_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HRD77090.1"
                     /translation="MSAKAGHEFDAEFQARPGNASRRARVKFDVRGLIIAADDRAEPH
                     IWPYPTLATPGAPSAGAPVHVTSTVEPGATLSVRDDLFRRALAQSVPGLAGSKPQRGP
                     ASTSMVLSILLTFALIGGIIYAAIAFLPYKTMARWIPEGTRETLGTLAKSSLTKGRKT
                     CEAPAGKAALDALVKRLTASLPATGSGKPVAFKVAVVDWDLVNAFAVMGNQVVLTKGL
                     LNTATSPDEVAGVLGHEMGHSLEMHPEAGVLRAVGTIAAIKILLGGWSPDIMTDMGAQ
                     VVLLRYSRSAETEADDVALKMLRDAKIAPKGFSNFFERLADKEKKGAAPSVPDIFSTH
                     PSSPERAKRAASQPAYPATPALSDAEWGALRNICGS"
     gene            complement(8770..9015)
                     /locus_tag="PK264_14365"
     CDS             complement(8770..9015)
                     /locus_tag="PK264_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77091.1"
                     /translation="MTTESHMRRLMDRIDQARERAAEAERLQAGAVTQLDTVDYELAA
                     IASELAAAGLIAKRPVAEAESKVAPESEAVPPVRRTA"
     gene            complement(9160..9921)
                     /locus_tag="PK264_14370"
     CDS             complement(9160..9921)
                     /locus_tag="PK264_14370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77092.1"
                     /translation="MDLKGAVAIVTGGNGGLGQRICLKLAEAGADIAVVCARSVKDAE
                     RVADDLARGKGGNQVRARAFAADLADPKAITKLVDDVMAHFGRIDILVNDAAYNIAIP
                     FPDLDRLTLEEWQKIMSINLTGPMLITKSVAPVMKRQGRGRIVNIASVAGLQPTGSSI
                     AYAVSKAGLIHLTRCMAVALAPEVLVNCVAPGLLEGTRATANLMPAQIERSASSSLLK
                     KAADKDDCADQVVAMCRTETMTGQTVVIDAGRVFH"
     gene            complement(9954..>10656)
                     /locus_tag="PK264_14375"
     CDS             complement(9954..>10656)
                     /locus_tag="PK264_14375"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRD77093.1"
                     /translation="GGRRPSGPEARMTSREKVLGRVKSALGPVTAERKAAVEARLKER
                     PRHLIPERAKHEAGKLKSQFKAFLEGQSATVLEVKSDADVPGAIAGFLRTANLPQRVK
                     HGADPYLAGLAWSKEPQLERLSGPANAKDEVSLSRVVTGVAETGTLVLASGAENPVTL
                     TYLPETHIAVLSAKDIVGPYEDAWQRVRAKFGEGVMPRTVNFVSGPSRTGDIGGQLVM
                     GAHGPRRMCVIIVDA"
BASE COUNT         1656 a         3625 c         3606 g         1769 t
ORIGIN      
        1 ccggcacgtc gtccttcagg cgcacgaggt cacggctcac gcgagcctgg tcggcgaact
       61 cgatgaggcg ctcgcgccgc ttcggctgct tgatctcgcc cgccttggcg agcagcgtgt
      121 cgaggtcgcc atactgcgtg atgagctcgg ccgcggtctt gatgccgatc cctggcacgc
      181 ccggcacgtt gtcgacggaa tcgcccgcaa gcgactgcac atccaccacc ttctcgggcg
      241 gcacgccgaa tttctcgatc acctctgcat gcccgatgcg gcggttcttc atggtgtcga
      301 gcatggcgac gttgggcccg acgagctgca tcaggtcctt gtcggaagag acgatcgtca
      361 cgtcgccgcc ggctgccacg gtctcgcggg cgtaggtcgc gatgaggtcg tcggcctcgt
      421 agccgtcctt ctcgacgagt gtcacgttga acgccgtcac cgcctcgcgg atcaaaggga
      481 actgcggcac gagttcctcc ggcggcggcg gccggttcgc cttgtacgcc tcgtagatct
      541 tggagcggaa ggtatcgcgc gaggtatcga agatgaccgc gatatggctc ggcccatcct
      601 tgccctttgc ctcctggatg agtttccaga gcatctggca gaagccgtgc acggcgccga
      661 ccggtaggcc gtccgagggc cgcgtcaggg ggggaagcgc atgaaaggcg cgaaagatat
      721 agcccgagcc gtcgatcagg tagacgtgcg agcccttgcc gaccggcttc gcgccgccgc
      781 ctgcctccga acccggtgtc gcctctgccg ctgccatcgt cttctcctcg ctgccgggac
      841 cttagcccga actcgtggcg cctgcacgag gctcgcgtcg ttgtcccccg cagccggcag
      901 cgatcgttcg cgggagggta ccaacgcttg atggcgatca aggcgcggca tgctacgcat
      961 gttcgaaggt gacattgccg gtcgcgatac tgcgatcaat ccgactcact gcggacagag
     1021 tggaggctcg acatgcgcgc gatactttcg aggccgaacg cccttctgct gagtgctgtc
     1081 gccgggggcg gcgccctggc gatgtcactg tccgtcgtcc cgggggccgc gcagagcccc
     1141 ggcgtcgtca gctacaacaa gaaggctgcc tacgacgacg tgaagtttga actcaacaac
     1201 gcgatcgtcg gccgtggcct cacgatcgat tacaacggtc agcttggccg gatgctcgaa
     1261 aggacgggcg ccgacgtcgg cagcaccaag ccgatctaca agaacgccga gtactttgcg
     1321 ttctgctcgg cgaagctctc ccgcgccgca atggaggccg acccggccaa catcgccggc
     1381 tgcccgttca tcgtcttcat ctatgagacg gccgcggcgc ccggcaccgt gcatgtcggt
     1441 taccgacgcc agccgtcgca tggtgcggca gcctccgtga aggcgcatgc ggatatcgat
     1501 gcgcttctcg atggcatcgc caaggctgcc gtgaagtagc cggatcgaac gtcgccgcgg
     1561 gcgcgatcag ttgccttcga tcggacccca gcgaagctcg aaaggcgaaa tctcgattgc
     1621 ctgctccttc ggcggcgcat tgaggaggcc gcgcgcggca gacgtcacct cggcggcaac
     1681 gcccgtgacg agatggctcg catgggcccg gaaggcgacg ttgcggcgct cgctcgcgag
     1741 ccggccatcg acccagagtc gcaagatccc gtctcgcttg ccgggcgtgt tgagcacgat
     1801 ctcctgctcg atctgcaccc attgcccctt cggcatcgag acgcgagccg ggccatagtc
     1861 gaggccttcg ggcgtctcga ggttgagctc gacgacgccg gccgcgcgcc acatcggccg
     1921 cgctgtgaaa gcgcgtgctt tctccggaac ggccgcgctg tcctgacgct cgccgcccat
     1981 gagtcccggg agccgtccgc cgcccgcgaa gtcgaaatct gccggcaggc gcacgcgata
     2041 gccgaggcag gccgcggcct tcggtgtcat ggtgcgcggc tcccagcgga aggcgacacc
     2101 gccacgcagc gcgctcttct cggctggatc gagcgagccc ttcggcaggg cgatgcgcat
     2161 tgcgtgcttg tgctcgaggc ccttcgccgg aacgaccctg aagttctcct tgatgcccca
     2221 atcgtcgcca gccaggcgag cctcgaaatc ctcggtcgtg aagggcgcgc cgctggcccc
     2281 ctcgaatgcg aagagcacgc cttccgcata gcgctcgccg cagggcgcct cggttgccgg
     2341 catcagggtc gagcgtacga tcacggcgag cgtgccgatg gcgacgagcg cgccgatcgc
     2401 attgaaggca taagccgttg gcgtcatctt catggggggt ctcgctcagc tgcggaaggc
     2461 gcggttgaca ttgcgcatga tgggatcgag cagctggtcg agcagcgtgc gcgcgcccgt
     2521 cacgaccagg atctcggcac gctggccggg gtggatgcgc tgctccttca caagctcgct
     2581 cttcggatcg gcaagctcga tgcgcgccga gaagaaggcg acgcccgagc gcttgtcctc
     2641 gacgctgtcg ggcgaaatcc aggcgacgcg ggcggccagc ggacgctgct cgcgccaggt
     2701 cgttcccgtg agccagactt tcgccggcat gcccggcttc acgaggtcga tgagggcgag
     2761 cgacaggcgg ccctcgatca cgagccggtc gtccttcggc acgatctcgg caaccgtctg
     2821 gcctggagtg agaacgccgc ccgtcccatg cacggcgagc atgaggaggc gccccgagac
     2881 gggggctcgg acctgcgcct tgcgggcgtc ctgctcggcg atcgagatgc gcgcaatgag
     2941 cgaggccgcc tctttttcca gctcagagag ctggcgctcg agcgcgagca gccgcgagcg
     3001 ctggatgagc tgcttggcga cgagttcgcc gatcgcctga acttcctggc ggacgagctc
     3061 catctggcgg ctcgccgcct gttgctgcgc acgcagggcc gcgatctgct cgccaagcgc
     3121 ggaggtatcg agggtgacga tcagcgcgcc ggcgtcgatc tcggcgcctt ccttcacgtg
     3181 aacctcggcg acgacgccgc cgcgatcatg ctggacggcg cgcgtccgtg tctcgacgac
     3241 gagcgtgcca gagaggctcg cgcccttgtc gatcggcgcg acggccgcgg cagcaagtgc
     3301 cccgccgacg ccgatggcca ggacagccgc gccgagcagc acgggcccgc ggatgtcgag
     3361 acggggcagt gatcgggagg tgccgggcag acgctccatg ggcgggcctc aatgggcggc
     3421 ctgaagccgc ggcggtgcgg cttcggtcgg ggctgcgtgg gggtgggact gaacggcggc
     3481 gacgggcttt cccgccatga ggcgcttggc aacctggtcg gccggtccgt agtcctcgac
     3541 ggcgccgttg gcgagaattg ccatgcgatc ggcgagcgag aggagacgct ggtcctgcgt
     3601 cgcgatgacg atgccgacgc cttcagcctt caagccggcg agcacattgc gcaagcggcg
     3661 cacgccgacc gcgtccaggc cgagctcggg ttcgtccagc acgacaatgc gcggttcgcc
     3721 atgcagggcc cgcgcgagtg ccacggcgcg ccgctccctg agcgacagcg cctcgccgcc
     3781 ggcaccgacc gcggtatcat agccattggg cagcgctgag agcatgtcgt gaacgccggc
     3841 gcgcatggca gcgcgcgcga cgcccatatg gcttgcgtcg ctgaagcggg cgatgttgtc
     3901 gtgcacggtc ccctcgatga ggatcggctc gggcatgtag ccgatcggcg gcagtgtgtc
     3961 gctccgctgc cagcgcccga cggcgacgcc gtcgagctgc acggcgccct gcgtcggcag
     4021 cagtgcgccc gcgatgaggc cggagaggct cgatttgcct gcgccgttcg gcccgacgat
     4081 ggcgaggcac atcgacggct caagttcgaa ggtgaggttg cgcagcgcag gtgtccgccg
     4141 cgtcgggtag tagtaggtga cgcccgcgat cgtgagccgc ccctcgggcg aatgtcctgc
     4201 ctcggcgatg ccggggggcc tgacggtatc ggcaatggcg cgcagccgct tgtaggcggt
     4261 ccaggcactg ccggcggcgc ggaacgatgt gaccgactgc tcgaggggcg cgagcgcccg
     4321 cccgagcagg atcgcggcgg cgaccagcac gcctggcgtg acgtcgccgc gaatgacgag
     4381 gaacgcgccg agcgcgtaga tggaaacctg cgccgcaagg cggatcgcgc gggcgacggc
     4441 cttgaggacg ctcgtgcgct tgccgagcga ataggcgttg gcgacgtgcg tccggttgtg
     4501 ggcctcccac tggctcgtgg cgcccgtcgc caagccgagg gcacccgcga tctggccgcg
     4561 ctcggcgagc atcgcccacc agcgctcggc ccgctcctgg cccttttggg cttcaggcgc
     4621 gacacgcgcc gcaagcaggc attgcaggag cccggtcccg aacaggagca ctgccgcaac
     4681 cgcgccggcc acacccagca gtggatgcag caacgccagc gtgacgagga agatcggcac
     4741 ccacggcgcg tcgaacagcg tgccgatggc accgctcgag atgaaggtcc tgaggccctg
     4801 cagcgcgcgt gcatcgtgct tcaggtcgat cgccgagctg cccgatcgca gccccgtctc
     4861 aatgaggaag ctgccgagca cgtggtcgag ccacaggccc gcgcggagca ggatgcggtc
     4921 gcgcacgatc tcgatgaggg cgagcgccag tgtcgcgccg ccgatcatga gcgtgagcag
     4981 gatgagcgtc tcgatcgagc ccgacggcac gaccgtctcg aacacctgca gcgtatagat
     5041 cggcatggcg agcatcagca cgttaatgcc gccgctgaag atcagcgccg ccaccagcgc
     5101 ccgacgcgcc tgcttcagga tctgccgcga ggtccgcatc cgatacgccc aacaccgccg
     5161 gaccggctgg accgtgcgtc ttccgacaat gcgtgaactt gctgaacgaa gattgaagag
     5221 cggtcattcg tgccccgcgc cgcccgcaat ggcgcggctg caaaggattg gttagggtcg
     5281 cggccaacca taccgaatcc aggaggacca cgctcatgaa ccgcctcgac aacaaggtcg
     5341 cactcgtctc cggcgccgcg cgcggcattg gcgctgcaac tgcgaggctc atggcgagcg
     5401 tcggcgcgcg cgtggtgatc ggcgatattc tcgaggagcg cggccgggag acggtgaagg
     5461 aaatccagtc ggagggcggc aaggcggcct tcgcccggct cgacgtaacg agcgaggcaa
     5521 gctggaacgg tgccatcggt cacgcgacgc gagagttcgg cggcctcgac atcctggtca
     5581 acaacgccgg catcttcctc ggcaaggact tcgaggaggc gagcctcgac gattggcacc
     5641 ggctcgtcgc catcaacatg acgggcgtgt tcctcggaac caagctcgcc gtgccggccc
     5701 tcaaggaaag tggcgccagg agcaagcacg gcagcgcaat cgtcaatctc gcctcgatcg
     5761 cggggcttgt cggctcgcag ctcgacccgc tctactcgat gaccaagggc ggcgtaacgc
     5821 tgttcacgaa atcgaccgcg ctcgccttcg gccgcaagcg ctatccgatc cgcgtgaact
     5881 cgatccaccc gggcgtgatc gacactgaca tgggcgcgca gacgttcgtc tcgcgggcgc
     5941 gcaatgccgg gacgaacgat gtttcctcgg cgcgggccgc ctcgaccagc atgcacccga
     6001 tcggccgcct cggcacggcc gacgacatcg ctcgcggcat cgtcttcctg gcgtccgacg
     6061 attcggcctt catgaccgga tcgggcatgg tcgtcgacgg cggcatcacg gcgcaatagg
     6121 cgcgatcggg cccgggcctg cgccaccgag gccgcgggta gggtgctgcc ttccggcaag
     6181 aagcttgcta gaggttccca cgcaagtgat gcctgcgggg gacgcgaggc gcttgccggg
     6241 cccgcttcat gactgatcaa gaccacgcgc tcgacgccgc caaggtgcgc cgcttcggca
     6301 tcatggcggc gatcgccatc gcgctcattt cggcggcggg catcgcctgg ttcacgatcg
     6361 gcggcgggaa ggcattcgtc accgggatcg tgaccgcgct cgaccggcaa gtgtcagcca
     6421 atttcatgcc ggccgtcgcg acattcctgg tcatcgcctt catcctgcag ctcttcgtgc
     6481 tgccgacggg cacgatcgcc atgctgacgg gcggcttcct gttcggcgcg ccgcttgccg
     6541 cggctctcta ctacggcgcc cagatactgg cagctcccgt ggtctacggc gcggtccgcc
     6601 tcggctttgg cagcttcgcc gacgagaagc tcgacggctt cgtgaagcgc tacctgcccg
     6661 ctcgcttcag ctccatcgtc gagatcgcac gctctgaagg gctgctcgcg gcaatctcgc
     6721 tccgcctcgc gcccgtgatc acgagcccgg tcgtgccgat cctcgcggcg gcgaccggca
     6781 tcaagctagc ggcactcatc gccgggacaa tcctcgtcgg ctgggtgcgt cccctgttct
     6841 gggcgagtgt cggtgcgacg gcgcacagcc ttgcagaagt cacaagctcg tccgaattcc
     6901 tgagcaaggt caatttgacg cctctgctgt tggccttcgc cgcggcggcc gcgatattcg
     6961 tactccgact ggtattgcgg ctccgcactg acaagtgatc cgactggcac gtgcaaggta
     7021 gctccatgtc gctcgagacg ttttcgaaca ggacgaccac ccggacgccg gcctacgccg
     7081 ccggcgccat ggtactcctc gccgcgcttg ctgtgatcat tgccgcactc gccttcgagc
     7141 acattggcgg acacgttccg tgcgagctct gtctcaggca gcgctggccg tactatgtcg
     7201 gcatcccgct ggccttcctg gccctggtgc tgctgtcggc cggccaggcg aaggcggccg
     7261 ccctcctctt cctcgcggca gcactcggct tcctgctcaa cgcgggcttc ggcgtgcatc
     7321 aggtcggcgt cgaatggggc tactggccgg gccctgcgac ctgctccgga aatgcgatgc
     7381 aaccgctcgg tctgcccgcc ggcggcgatc tcttgaaatc gctgcagacg gtccggattg
     7441 cgcgctgtga tgcgccgaca tggcatttcc tcggcctgtc gttcgccggc tggaacgtgc
     7501 cgatctcact cgcgctggca gtcggcgccc tcaaggccgc ctatgatgcg ctcggccggt
     7561 ccgcggtccg actttgaggg tacgagcaac ttcggctatg gctcgggcga cgtccgcacc
     7621 gagtttccag gggtgatctg catgtctgcc aaagctggtc atgagttcga cgccgagttc
     7681 caggcgcggc ccggcaacgc ctcgcggcgc gcgcgcgtca aattcgatgt gcgcggcctg
     7741 atcattgccg ccgacgaccg ggccgagccg cacatctggc cctacccgac gctggcgacg
     7801 ccgggcgctc cgtccgctgg tgcgcctgtc cacgtgacct cgacggtcga gcccggcgcc
     7861 accctgagcg tgcgcgacga cctcttccgc cgggcactcg cccagtccgt accgggcctt
     7921 gccggtagca agccgcagcg cggacccgcc tcgacaagca tggtgctctc gatcctgctg
     7981 accttcgccc tcatcggcgg catcatctac gcggcgatcg cattcctgcc ctacaagacc
     8041 atggcgcgct ggattcccga aggcacgcgc gagacgctcg gcacgctcgc caagtcgagc
     8101 ctcaccaagg gccgcaagac gtgcgaggcg ccggccggca aagcggcact cgatgcgctc
     8161 gtgaagcggt tgaccgcgag cctgccggcg accgggtccg gaaagcctgt cgcgttcaag
     8221 gttgcggtcg tcgactggga cctcgtcaat gcctttgcgg tgatgggcaa tcaggtcgtg
     8281 ctgaccaagg gcctcctcaa cacggcaacc tcgccggacg aggtcgcggg cgtgctcggc
     8341 cacgagatgg ggcactcgct cgagatgcac cctgaagccg gcgtgctgcg cgccgtcggc
     8401 acgatcgcgg caatcaagat cctgctcggc ggctggtcgc ccgacatcat gacggacatg
     8461 ggcgcacagg ttgtgctgct gcgctactcg cggagcgccg agacggaggc ggacgacgtc
     8521 gcgctcaaga tgctgcgcga cgccaagatc gcgccgaaag gcttctccaa tttctttgag
     8581 cggctcgccg acaaggagaa gaagggcgca gccccctcgg tgccggacat cttctcgacg
     8641 catccctcta gcccggagcg agccaagcgc gcggcttcgc agccggccta tccggctacg
     8701 ccggcgctca gcgatgccga gtggggcgcg ctccgcaaca tctgcggctc ctgaacgagc
     8761 gatcgacgct caggcggtgc ggcgaaccgg cggaaccgcc tcgctctcgg gcgcgacctt
     8821 gctctcggcc tcagcgaccg gacgcttcgc gatcagtccg gcggcggcca gttcgctcgc
     8881 gattgccgca agctcgtagt cgacggtatc gagctgcgtc acggctccgg cctgcaggcg
     8941 ctcggcctcg gcggcgcgct cgcgcgcctg gtcgatgcga tccatcaggc gacgcatgtg
     9001 cgattcggtc gtcatcctca cctcgccggc cgtcaattgc ccgactgggg ataacgcgga
     9061 accacctgtg gaaagattaa actgattcgc cgcatgccgc gatcccggat gcgggcaaag
     9121 gctcgcgcgc tccggaggcg ttgagaagct accgacgcgt cagtggaaga cgcggcctgc
     9181 gtcgatgacg acggtctggc ccgtcatggt ctcggtgcgg cacatggcga cgacctgatc
     9241 agcgcagtcg tccttgtcgg cggccttctt caagagtgag gagctcgccg agcgctcgat
     9301 ctgggccggc atcaggttcg ccgttgcgcg cgtcccttcg agcagccccg gcgccacgca
     9361 attgacgagc acctccgggg cgagcgccac cgccatacag cgcgtcagat ggatgaggcc
     9421 cgccttcgag accgcgtagg cgatcgacga gccggtcggc tgcagccccg cgaccgaggc
     9481 gatgttgacg atgcggccgc gcccctggcg cttcatgacg ggcgccacgg atttcgtgat
     9541 cagcatcggc ccggtgaggt tgatcgacat gatcttctgc cactcctcga gagtcagtcg
     9601 atcgaggtcg ggaaagggaa tggcaatgtt gtaggcggcg tcgttgacga ggatgtcgat
     9661 ccggccgaag tgcgccatca cgtcgtcgac gagcttagtg atggctttcg ggtcggcgag
     9721 gtcggcggca aatgcccggg cccgcacctg atttccgcct ttgccgcggg cgaggtcgtc
     9781 ggcgacccgc tcggcgtcct tcacgcttcg cgcgcagacg acggcgatgt ctgcgccagc
     9841 ctccgcgagc ttcaggcaga tgcgctggcc gagcccgcca ttgcctccgg tgacgattgc
     9901 gactgcgcct ttgaggtcca tggcatctgt cctttggctt tggctctgct gtctcaggcg
     9961 tcgacgatga tcacgcacat gcgtcgcgga ccatgcgcgc ccatgacgag ctggccgccg
    10021 atgtcaccgg tccgcgaggg gccggagacg aaattcaccg tgcgcggcat cacgccctcg
    10081 ccgaacttcg cgcgcacgcg ctgccaagca tcctcgtagg ggccgacgat gtcctttgcg
    10141 gacagaaccg cgatgtgcgt ctcggggaga taggtgagcg tcacggggtt ctcggcgccg
    10201 gacgcgagga ccagcgtgcc ggtctcggca actccagtca cgacgcggct gaggctcacc
    10261 tcgtccttgg cgttcgctgg gccggacaag cgctcgagct gcggctcctt cgaccaggca
    10321 agccccgcga gataggggtc tgcgccgtgc tttacgcgct gcggcaggtt ggctgtgcgc
    10381 aggaagcccg cgatcgcacc cggtacatcg gcgtccgatt tcacctcgag gacggtcgcc
    10441 gactggcctt cgaggaacgc cttgaactgc gacttgagct tccccgcctc gtgcttcgcg
    10501 cgctcgggga tgaggtggcg cggccgctcc ttcaagcgag cctcgacggc ggccttccgt
    10561 tcggctgtta ctggccccaa ggcggacttg acccgtccga gcaccttctc gcggctcgtc
    10621 atgcgggcct ccggtcctga tggtcgacgc ccgcct
//
LOCUS       DAORNH010000142        10637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4643_length_10637_cov_3.004347, whole genome shotgun sequence.
ACCESSION   DAORNH010000142 DAORNH010000000
VERSION     DAORNH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4643_length_10637_cov_3.004347"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /gene="soxX"
                     /locus_tag="PK264_14380"
     CDS             <1..424
                     /gene="soxX"
                     /locus_tag="PK264_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HRD77094.1"
                     /translation="PVRADALTIMAREKARIVYPADGKFMGDWKRGEALAQSGYGLRF
                     TDRDTKRANGGNCYACHQLAPAEVSYGTVGPTLREYGKIRKYDEREIRAVYEKIYNSH
                     AAFACSMMPRFGSSGVLTIDQIKDLVALLMDPESPVNK"
     gene            585..1091
                     /locus_tag="PK264_14385"
     CDS             585..1091
                     /locus_tag="PK264_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HRD77095.1"
                     /translation="MPVTMHSVSLPLFSRMLSNLSAILDKAEAYAAAKKIDPEVMLNA
                     RLAPDMFPLKRQVQLASDFAKGAAARLSGQDIPKWDDSEKSFAELKARLKRTQDYLAG
                     FGAAQIDGSEARDVSLTIAQKPAVLKGQAFLTNYAFPHFFFHVTTAYNILRHNGVEVG
                     KRDFMGPF"
     gene            1102..2172
                     /locus_tag="PK264_14390"
     CDS             1102..2172
                     /locus_tag="PK264_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77096.1"
                     /translation="MAAQADDGRRAQPTAAFAAVGDLYHAYITGLMLTLVTRRSAADA
                     AEWVFRLFRHQHHEKFLSSLEKLGIVALPDAVKCAAYHYLSNSVGGVTVEFIRESDRK
                     AWVHFVPPRWIYPGAAICGIPSEVSRAMLRGWYAENGVSLRNPRLGFVCTAQTTDGQH
                     GLAGYFLEHDRDLRAEERLRFNPGELAPPFDPALAPKLDGNAWSPERLAKAQRNYAME
                     YIRSGLPRLVELFGPAEARHLGRTTGQLIGAQLSRATAAALGIAGRDAASLAALLQGL
                     ARAEGDDAEVAREGVDILVSRDGWRLMRGISRPSPAVFDAWNGLNEGLAAVHNRDLRL
                     DVLARADEGDERFVWRIRARRA"
     gene            2221..2634
                     /locus_tag="PK264_14395"
     CDS             2221..2634
                     /locus_tag="PK264_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD77097.1"
                     /translation="MSIDAWSFVHNLAQKAEWTPGLREIFEYRDLGIKDGTKGDYVAH
                     LIRHNGKKVADQVQQWHVHDCTFQFVYVMSGWATFEYEGQGQRTIRKGDSILQTPGIK
                     HREIACSEDFEVLEIVAPADFKTRVVEAPKANAAE"
     gene            complement(2694..>2926)
                     /locus_tag="PK264_14400"
     CDS             complement(2694..>2926)
                     /locus_tag="PK264_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HRD77098.1"
                     /translation="PRHDDQSDIKNTFKLQRALGGLVETVVLDDSYHMVTLDRQRGLV
                     SERSISFAESIKKRVEAIAAMERARRGQVAAE"
     assembly_gap    2927..2954
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2955..3573)
                     /locus_tag="PK264_14405"
     CDS             complement(<2955..3573)
                     /locus_tag="PK264_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRD77099.1"
                     /translation="MKNKDNLSSSAGDLVLPGGRTGFLLFHGLGGSPVELRFLAQGLN
                     RAGYTVHAPLLSGHGGSSADLEASTWRDWYASAEAALVRLREHCDVVIAGGISVGAVL
                     ALRIAAHRTTDVDATALYAPTLWPNGWAIPRSMELYKLINDRFSASFFRFRERAPYGI
                     KDERIRKFVLDSLQANGTPIDEIFGRSGNVVLQFRWLVKDMMRTVG"
     gene            4122..4925
                     /locus_tag="PK264_14410"
     CDS             4122..4925
                     /locus_tag="PK264_14410"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HRD77100.1"
                     /translation="MTQLWALHRTGSLSLMFAGMASLLAVALARAEAPASPRVAIASV
                     VELFTSQGCPKCPPADALLKTYADRPGVLAVTYHVDYWDYLGWRDTFASAFFTERQRG
                     YAAIQGDGMIYTPQVVINGRAHTTGSKAPKIDAAITATTRAFADARISVDLVHRSKMI
                     VVKVGARTATAAPQPDTAKGVDMATVWLLAIQKSATVKIGHGDNSGRTAVYTNVVRRM
                     VPVGSWTGATLEVKLDSDAVKIPGVDACAILVQSGVTGPLLGATPIIDL"
     gene            complement(4948..5538)
                     /locus_tag="PK264_14415"
     CDS             complement(4948..5538)
                     /locus_tag="PK264_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77101.1"
                     /translation="MMRFLMIGSLAVAAMSALLLPQPGVALEEKKGEKEALKACETRT
                     CDIVAKKGGSDLVCSIGRTWKKDKIKAGFGARVAWRWGDAQCSGDINVPAALIADALT
                     RPEHKITVPLHTVTCLLENETGTVPLKITLAPTIQTRGGRARKVWINVKSIEGPGVIK
                     STIWTVVRLEDSIGIFHGDIVKAVNKFIDRCQAPKT"
     gene            5787..5930
                     /locus_tag="PK264_14420"
     CDS             5787..5930
                     /locus_tag="PK264_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HRD77102.1"
                     /translation="MSSDTTHGHPEMDYREHNRTYAGFIRGTQILVVLLALLLLGMAL
                     FLA"
     gene            5979..7118
                     /locus_tag="PK264_14425"
     CDS             5979..7118
                     /locus_tag="PK264_14425"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HRD77103.1"
                     /translation="MVTIAVAGEGADEPRVAISPETVKKFVGLGATVRVAAGAGRRAR
                     VSDDALKAAGAEIVAQPGAAASGADILLSVRRPSLESVKALKKGGLVFAMLDPYSERS
                     GLEELAAEGAGLFSMEFMPRITRAQSMDVLSSQANLAGYKAVVDAASMFEQAMPMMMT
                     AAGTVPAAKVFIMGVGVAGLQAIATARRLGAIVSATDVRPATKEQVASLGAKFIAVED
                     DEFKQAQTAAGYAKPMSEAYQKKQAELVATHIKGQDIAITTALIPGRPAPRLISRAMV
                     ESMKPGAVIIDLAAERGGNCELTQPGKVVDHNGVRISGPINLPGSVPVNASSLYAKNL
                     LTFLDTMFDKKAKSFAVNWEDEIVKGTLVARDGKIVHPNLQPKGA"
     gene            7168..7524
                     /locus_tag="PK264_14430"
     CDS             7168..7524
                     /locus_tag="PK264_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HRD77104.1"
                     /translation="MVAVAGLVDTALAAGGSDAVSPTVYQLMVFVIAIFVGYYVVWSV
                     TPALHTPLMSVTNAISSVIVVGALLAVGVSTLSSGSYIAKFFGFLALIMAAINIFGGF
                     LVTQRMLAMYKKKDGK"
     gene            7521..7775
                     /locus_tag="PK264_14435"
     CDS             7521..7775
                     /locus_tag="PK264_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRD77105.1"
                     /translation="MTVDGRLPTWTAPIIFGALLTCLMSLVVAGVATWKALGFSPDFF
                     ARWLAAWLPSWAVAFPTVLVVAPLVRRIVARVTIPPAASR"
     gene            7795..9207
                     /locus_tag="PK264_14440"
     CDS             7795..9207
                     /locus_tag="PK264_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HRD77106.1"
                     /translation="MSANVVALLYLASGVLFIQALRGLSSPATSRQGNYLGMIGMTIA
                     IVTTLALLGAPGDVLTWFLIILALGIGGGIGAYTARTIPMTMMPELVAAFHSLVGMAA
                     VLVAAGALYAPTAFGIADTSGRIGAASLLEMSLGVAIGAITFTGSVIAFLKLSGRMSG
                     KPIILPLRHLINGALAALIIYLIYIFCQSGGSAWLFWLITLLSLVFGVLIIIPIGGAD
                     MPVVVSMLNSYSGWAAAGIGFTLGNIALIITGALVGSSGAILSYIMCKGMNRAFLSVI
                     AGGFGGETAGPSGEKGEQKPVKLGAADDAAYILKNASKVIIVPGYGMAVAQAQHALRE
                     MADNLKKAGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFSQTDAVY
                     VIGANDVVNPAAEEDKSSPIFGMPVLQVWKAGTVLFNKRSLASGYAGIDNTVFYRDNT
                     VMVLGDAKKMTEEIGKALAH"
     gene            complement(9233..10000)
                     /locus_tag="PK264_14445"
     CDS             complement(9233..10000)
                     /locus_tag="PK264_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77107.1"
                     /translation="MLALGIRCHSMAPLHGQTLTGPSAGIEGIFMTGGNDGGMAHVSE
                     AAGHGGTWAATMISAAAFVFSGISFYMSALQRAELEVYVPPVLQYARDGGGDVDVFAI
                     PITIANNGANTGAVLALELVVENVKEGAEPKSKTFYSAFMGEHPRDPAASNKTFAAIA
                     IPGRGTFSDTVRFYPRGNPLPKIVQDAGEYRFTLRLVVAEPPAPGLVDRMLGTAAPAP
                     ITFTRTIPYISEQHLGFRRGTVIMHDKDWKPMSSAGK"
     gene            10044..>10637
                     /locus_tag="PK264_14450"
     CDS             10044..>10637
                     /locus_tag="PK264_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD77108.1"
                     /translation="MAEKFVTIERGLGPEGRIALVRFDRGDRINALSPEAMRQLRAAA
                     QSFDDDLETSVVVLTGNAHAFSAGFDLKDAERKAKAAQGRKSIRVGPAMCKAWAEMEP
                     VTICAIEGFAIGGGAALAVSLDFRILSRAAHLRIPELALGMNMSWGTIPRLLQLIGPA
                     RTKQAVICAEDRIEAREALDWRLVEELADPGAALAAAM"
BASE COUNT         1784 a         3510 c         3425 g         1890 t
ORIGIN      
        1 gcccgtgagg gcggacgcgc tcaccatcat ggcgcgcgaa aaggcgcgca tcgtctaccc
       61 ggccgacggc aagttcatgg gcgactggaa gcgcggtgag gcgctcgccc agagcggcta
      121 tggcctgcgc ttcacggacc gcgacacaaa gcgggcgaac ggcggcaact gctatgcctg
      181 ccaccagctg gcgccggccg aggtcagcta cggcacggtc ggcccgacgc tgcgcgagta
      241 tggcaaaatc cggaagtacg acgagcgtga gatccgcgcc gtctacgaga agatctacaa
      301 ctcgcacgcg gcgttcgcct gctcgatgat gccgcgcttc ggctcgagcg gtgtcctcac
      361 catcgaccag atcaaggatc tcgtagccct gctgatggac cccgagagcc cggtcaacaa
      421 gtagtgatcg ggcgttgccg cggcaggctt cacgtccgtc atcgggcgtg caaaggtcgc
      481 ggcgcgttca cgatcatggc gaggagcgcg tcggctgatc ggccggcgcg ctgtcgttgt
      541 cgccatgctc caatctcatc acgcagttca aactggagcc ccgcatgccc gtcacgatgc
      601 actccgtatc tcttccgctg ttctcgcgga tgctttcgaa cctctcggcg atcctcgaca
      661 aagccgaggc ttatgcggcg gcaaagaaga tcgatcccga ggtcatgctg aatgcgcgcc
      721 ttgcgccgga catgtttccg ctgaagcggc aggtgcagct cgcctctgac ttcgcgaagg
      781 gtgccgctgc gcgcctctcg ggtcaggaca ttccgaaatg ggacgacagc gagaagtcgt
      841 ttgctgagct caaggcacgt ctgaagcgca cgcaggacta cctcgcgggc ttcggggctg
      901 ctcagatcga cggttcggag gcgcgcgacg tcagcctgac gatcgcccag aaaccggcgg
      961 tgctgaaggg ccaggccttc ctcaccaact acgcgttccc gcacttcttc ttccacgtga
     1021 ccacggccta caacatcctg cgccacaacg gcgtcgaggt cggcaagcgc gatttcatgg
     1081 gaccgttctg agcgttccgt gatggctgcg caagccgatg acggacgacg tgcgcaaccg
     1141 acggcggcat tcgcggccgt cggcgacctc tatcacgcct acatcaccgg gctgatgctg
     1201 accctcgtca cccggcgctc ggcggcggat gccgccgagt gggtgttccg tctcttccgc
     1261 caccagcatc acgagaagtt cctctcgagt ctcgagaagc ttggcatcgt ggcacttccc
     1321 gatgccgtga agtgcgcggc ctaccactac ctctcgaact cggtcggcgg cgtcacggtc
     1381 gagttcatcc gcgagagcga ccgcaaggcc tgggtgcatt tcgtgccgcc gcgctggatc
     1441 tatccgggag ccgcgatctg cggcatcccg agcgaggttt cgcgcgcaat gctgcgcggc
     1501 tggtacgccg agaacggcgt ctcgctcagg aacccgcggc tcggtttcgt ctgcaccgcg
     1561 cagacgacgg acggtcagca cggcctcgcc ggctactttc tcgagcacga tcgcgatctc
     1621 agggccgagg aacgtcttcg cttcaaccca ggcgagctcg ccccgccctt tgatccggct
     1681 ttagcgccga agctcgacgg caacgcctgg tcgcccgagc ggctcgccaa ggctcagcgc
     1741 aactatgcga tggagtacat ccgttcaggc ctgccccggc tcgtcgagct cttcggtccc
     1801 gccgaggcgc gccacctcgg gcggacgacg ggccaactca tcggcgcgca gctttcgcgc
     1861 gcaacagccg cggcgctcgg cattgcgggc cgtgatgcgg cgagcctggc tgcgctcctg
     1921 cagggcctcg cacgcgccga gggcgacgac gccgaggtcg cgcgcgaggg cgtcgacata
     1981 ctcgtcagtc gtgacggctg gcggctcatg cgcggcatca gccgaccttc gcccgccgtc
     2041 ttcgatgcct ggaatggcct caacgagggt ctcgccgccg tgcacaatcg tgatttgcga
     2101 ttggatgtgc tcgcgcgcgc cgacgagggg gacgaacgct ttgtctggcg catccgggcc
     2161 aggcgggcgt aggatcgcga agggtttcgc ccatcgccat caccgcaggg agcaatcgcc
     2221 atgtcgatcg atgcctggtc attcgtccac aatctcgccc agaaggcgga gtggacgccc
     2281 ggattgcgcg agatcttcga gtaccgcgat ctcggcatca aggacggcac gaaaggcgac
     2341 tatgtcgccc atctcatccg ccacaacggc aagaaggtcg ccgatcaggt ccagcagtgg
     2401 cacgtgcacg actgcacgtt ccagttcgtc tacgtgatga gcggctgggc gacgttcgag
     2461 tacgaggggc agggccagcg cacgatcagg aagggcgaca gcatcctcca gacgcccggc
     2521 atcaagcatc gcgagatcgc ctgctcggag gacttcgagg tgctcgaaat cgtcgcgccc
     2581 gcagacttca agacacgcgt ggttgaggcg ccgaaggcca acgccgccga gtgaaaggag
     2641 ggacggcgcg cgagcagctc gcgcgccgtg gtcctcgctc gatcggcccg tgcctactcc
     2701 gcggcgacct gaccgcgccg cgcccgctcc atggcggcga tcgcctcgac gcgcttcttg
     2761 atgctctccg cgaagctgat agagcgctca ctgacgaggc cgcgctgccg gtcgagcgtc
     2821 accatgtgat agctgtcgtc gaggacgacg gtctcgacga ggccgccgag cgcgcgctgc
     2881 agcttgaacg tgttcttgat gtcgctctgg tcgtcatggc ggggatnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnntgccga ccgtgcgcat catatccttc acgagccagc ggaactgcag
     3001 cacgacgttg ccgctgcgcc cgaagatctc gtcgatgggc gtgccgttcg cctgcaggct
     3061 gtccagcacg aacttgcgga tgcgctcgtc cttgatgccg tagggcgcgc gctcgcggaa
     3121 gcggaagaag ctcgccgaga agcggtcgtt gatgagcttg tacaactcca tcgagcgcgg
     3181 gatggcccag ccgttgggcc acagcgtcgg agcgtagagg gcggtcgcat cgacatcggt
     3241 cgtccggtgg gccgcgatgc gcagcgccaa cacggcgccg accgagatgc cgccagcgat
     3301 gacgacgtcg caatgctcac gcagccgaac gagcgccgcc tcggccgacg cataccaatc
     3361 gcgccaggtc gatgcctcga ggtctgctga tgagccaccg tggccgctca gcaacggcgc
     3421 gtgaacagtg tatcccgctc tattcaatcc ttgcgccagg aaccgcagct ccaccgggct
     3481 tccgccaagc ccatggaaca acaggaaccc ggttcgcccg ccgggaagca cgagatcgcc
     3541 ggccgacgac gataaattat ctttattttt catactgata cgtggcctcg ttctagttat
     3601 tgcgcaggat tgatagagaa attaatcctg ttcgccaagc gaaaactgtc ttgcgcagac
     3661 taatggaggg ccgctccact gtcggaccgc gctgcaccgt tcgtcagcgg cgcgatgctc
     3721 tgccagctcc cgagcattcg atccacgagg catgatgcac atggtaaaag aacacgtttt
     3781 caccggctga tgccgccgcc atggacgggc tgccatcgcg cgcccacaat ctacaggccg
     3841 ccacggctcg ccacgtcaac gcatcgtgac tggcaattga gtacgcgagg aacgataagg
     3901 aggatgacgt tctgagtgtc gaccgttgta tcgagcgcga tgcaggcgcc tcgaccgacg
     3961 ctacagggcg aacattcaaa ttgtcttcgg accatcgcca atggcggtcc gttcagccgg
     4021 ggcaggtaag acacttgccg cggcggcccc gctacagaat gtcggggaag caggtggaat
     4081 gccggatgag accgcgtgag cgaaaggcga gcagtcagcg catgacacag ctttgggcac
     4141 tgcatcggac agggtcgctg tcgctcatgt tcgccgggat ggcgtcgctc ctcgccgtcg
     4201 cgctcgccag agccgaggca ccggcatcgc cccgcgtggc cattgcgtcg gtcgtcgagc
     4261 tgttcacgag ccagggctgc ccgaaatgcc cgccggccga tgcgctcctg aagacctatg
     4321 ccgaccggcc gggcgtactc gccgtcacct atcacgtgga ctactgggat tatctcggct
     4381 ggcgggacac gttcgcgagt gcattcttca ccgagcgcca acgtggctac gcggccattc
     4441 agggcgacgg catgatctat acgccgcagg tggtgatcaa cgggcgcgcg catacgaccg
     4501 gcagcaaggc gcccaagatc gacgccgcga tcaccgccac aactcgtgcc ttcgccgacg
     4561 ctcgcatctc cgtcgatctc gtacatcgca gcaagatgat cgtcgtcaag gtgggggccc
     4621 gcacggcgac ggccgccccg caaccggaca ccgcaaaagg tgtcgacatg gcgacggtct
     4681 ggctgctcgc catccagaag tcagcgaccg tcaagatcgg ccacggcgac aattcgggcc
     4741 ggacggccgt ctacaccaat gtcgtgcgcc gcatggttcc ggtcggctcg tggacgggtg
     4801 ccaccctcga ggtgaagctc gacagcgacg cggtgaagat ccccggggtc gatgcgtgcg
     4861 ccatcctcgt ccagtcgggc gtgaccggtc cgctcctcgg tgcgacgccg atcatcgacc
     4921 tgtgacggga aatcgccgtc gagcaggtca ggtcttgggc gcctgacaac gatcgatgaa
     4981 cttgttcacg gccttgacga tatcgccgtg aaagatgccg atcgaatcct cgagccggac
     5041 gaccgtccag atcgtgctct tgatgacgcc cggcccctcg atcgacttga cgttgatcca
     5101 tacctttcgg gcgcgccctc cgcgggtctg gatggtcggc gcaagtgtta tcttcagcgg
     5161 caccgtcccc gtctcgttct cgaggaggca ggtcaccgta tgcagcggca ccgtgatctt
     5221 gtgctccggc cgggtcagcg cgtcggcgat cagtgcggcc gggacattga tgtcgcccga
     5281 gcactgggca tcgccccaac gccacgcgac ccgcgcaccg aagcccgcct tgatcttgtc
     5341 cttcttccag gtccgcccga tgctgcagac gagatcgctg ccgcccttct tggcgacgat
     5401 gtcgcaagtc ctggtctcgc aggccttgag cgcttccttc tcccccttct tctcctcgag
     5461 cgcgacgccg ggttgcggca gcaggagtgc cgacatggcc gcgacggcca gcgagccgat
     5521 catcagaaag cgcatcattg agaccccaat caggttcact cgctggccga gggcacgctc
     5581 cctcggccac ccaggactaa cgcgaaggcg gcgtacctgc catcgcgacc atggcacaat
     5641 gacggcggag cgagcgagcg tcgcctcctt gccggagcgg ctcgcatgag ccactgttgt
     5701 cgcaccgcac cagtcggctt gctggcctga tgcgattgag ctacgaacgg gaaccatgga
     5761 tttgctttgg ctctgggagg cccttgatga gctcggacac aacgcacggt catcctgaga
     5821 tggactaccg cgagcacaac cgcacttatg ccgggttcat ccgcggcacg cagatcctgg
     5881 ttgtgctcct cgcgctgctg ctgctcggca tggcactctt cctggcttga tcgagacttc
     5941 cggtccgtgc gtcgggcatt gctctttggg gggccgtcat ggtgacgatc gccgttgcag
     6001 gcgaaggggc tgacgagcct cgggtggcga tatcgcccga gacggtgaag aagtttgtcg
     6061 ggcttggggc caccgtccgc gtggctgccg gggctgggag gcgcgcgcgc gtctctgatg
     6121 atgcgttgaa ggccgccggc gccgagatcg tcgcgcagcc gggggcggcc gcttccggtg
     6181 ccgatattct cttgagcgtg cgtcgcccct ccctcgaatc tgtgaaggcg ctgaagaagg
     6241 gcggcctcgt attcgcgatg ctcgatccct acagcgagcg ctccggcctc gaggagctcg
     6301 ccgcggaggg tgccgggctc ttctcgatgg agttcatgcc gcgcatcacg cgtgcgcagt
     6361 ctatggacgt actctcgagc caggcgaacc ttgccggcta caaggcagtc gtcgatgcgg
     6421 cctcgatgtt cgagcaggcg atgccgatga tgatgacggc cgccggaacg gtgcccgcgg
     6481 ccaaggtgtt catcatgggc gtcggcgtcg cgggtctgca ggcaatcgcc accgcgcgtc
     6541 gcctcggcgc catcgtctcc gcgaccgatg tccgtccggc tacgaaggag caggtcgcgt
     6601 ctctcggcgc aaagttcatc gccgtggagg acgacgagtt caagcaggca cagaccgcgg
     6661 cgggctatgc gaagcccatg tcggaggcct accagaagaa gcaggctgag ctcgtcgcaa
     6721 cccacatcaa gggccaggac atcgccatca ccacggcgct tattccgggc cggccggcgc
     6781 cgcgtctcat ctcgcgcgcg atggtcgaga gcatgaagcc cggcgccgtc atcatcgatc
     6841 ttgcggcaga gcgtggtggc aactgcgagc tgacccagcc cggcaaggtc gtcgaccaca
     6901 acggtgtgcg catctcgggg ccgattaacc tgccggggtc cgtcccggtc aatgcctcga
     6961 gcctctatgc caagaacctg ctgaccttcc tcgacaccat gttcgacaag aaggcgaagt
     7021 cgttcgcggt caactgggag gacgagatcg tcaagggcac gctcgtcgcc cgtgacggca
     7081 agatcgttca tcccaatctg cagccgaagg gagcgtgaga tgagggcaag cctgatccgc
     7141 cgcgctggac tgctcctggt tgcgttcatg gttgcagtgg ccggcctcgt cgacacggcg
     7201 ctcgccgcgg gcggctctga tgccgtgagc ccgaccgtct accagctgat ggtgttcgtg
     7261 atcgccatct ttgtcggcta ttacgtcgtg tggtcggtga cgcccgcgct gcacacgccc
     7321 ctgatgagcg tgaccaacgc catctcctcc gtgatcgtgg ttggcgcgct gctcgccgtc
     7381 ggcgtctcga cgctctcgtc cggctcctac atcgccaagt tcttcggctt cctcgcgctg
     7441 atcatggcgg cgatcaacat cttcggcggc ttccttgtca cgcaacgcat gctcgcgatg
     7501 tacaagaaga aagatggcaa atgaccgttg acgggcgctt gccgacctgg acggcaccca
     7561 tcattttcgg cgcgcttctg acgtgcctca tgtctcttgt ggtcgccggc gttgcgacat
     7621 ggaaggcgct cggcttctca cccgacttct tcgcgcgctg gttggcggca tggctgccgt
     7681 cctgggccgt tgccttcccg actgttctcg tggttgcccc cctcgttcgc cgcatcgttg
     7741 cccgcgtgac gataccgcca gccgcctccc gctgaggagc ctctgtctat gaccatgtcc
     7801 gccaacgttg tcgcgctcct ctaccttgcc tcgggcgttc tgttcatcca ggcgctgcgc
     7861 ggattgtcga gcccggcgac cagccgccag ggcaactatc tcggcatgat cggcatgacg
     7921 atcgccatcg tcacgacgct cgcgctgctc ggtgcgccag gtgatgttct cacctggttc
     7981 ctgatcatcc tggcgctcgg catcggcggc ggcatcggcg cctacaccgc ccgcaccatc
     8041 ccgatgacca tgatgcctga gctggtggcg gcgttccatt cgctggtcgg catggccgcc
     8101 gtgctggtcg ctgccggcgc gctttatgct ccgacggcgt tcgggattgc cgacacgagc
     8161 ggccgcatcg gtgccgcgag cctgctcgag atgagcctcg gcgttgcaat cggcgcgatc
     8221 accttcacgg gctccgtgat cgccttcttg aagctctccg gccgcatgtc agggaagccc
     8281 atcatcctgc cgctccgcca tctcatcaac ggcgcgctcg cggccctcat catctatctc
     8341 atctacatct tctgccagtc tggcggctcg gcgtggctct tctggctgat caccctcctc
     8401 tcgctagtct ttggcgtact catcatcatc ccgatcggcg gcgccgacat gccggtcgtc
     8461 gtgtcgatgc tcaactccta ttcgggctgg gccgccgccg gcatcggctt cacgctcggc
     8521 aatatcgcgc tcatcattac aggcgcgctg gttggctcgt cgggcgcgat cctctcctac
     8581 attatgtgta agggcatgaa ccgcgccttc ctttccgtga tcgccggggg gttcggtggc
     8641 gagacggcgg gaccctcggg cgagaagggc gagcagaagc ccgtgaagct cggcgcagcg
     8701 gacgacgccg cctacatcct gaagaacgcc tcgaaggtga tcatcgtgcc gggctacggc
     8761 atggcagtcg cccaggctca gcacgcgctt cgcgagatgg ccgacaacct gaagaaggcc
     8821 ggtgtcgagg taaagtacgc aatccatccc gtcgcgggcc gcatgccggg ccacatgaac
     8881 gtgctgctcg ccgaggcgaa cgtgccctac gacgaggtgt tcgagctcga ggacatcaac
     8941 tccgagttct cgcagacaga cgccgtctac gtgatcggcg ccaacgacgt cgtcaatccg
     9001 gcggcggagg aggacaagtc ctcgccgatc ttcggcatgc cggtgctgca ggtgtggaag
     9061 gccggcaccg tgctcttcaa caagcgcagc ctcgcatccg gctatgccgg catcgacaac
     9121 accgtcttct accgcgacaa cacagtgatg gtgctcggcg acgccaagaa gatgaccgag
     9181 gagatcggca aggcactcgc gcactgaccg gcggctcagc gggctcccgc gcttacttcc
     9241 cggcgctcga catcggcttc cagtccttgt cgtgcatgat cacggtgccg cggcggaagc
     9301 cgaggtgctg ctcggaaatg taaggaatcg tccgcgtgaa ggtgatcggc gccggggccg
     9361 ctgtcccgag catccggtcg accagcccgg gggcgggcgg ttccgccacc acgaggcgca
     9421 gcgtgaagcg gtactcgccg gcatcctgca cgatcttcgg cagaggattg ccgcgcgggt
     9481 agaagcggac cgtatccgag aacgtgccgc ggcccgggat ggcgattgcc gcgaacgtct
     9541 tgttcgaggc agccgggtcg cgggggtgct cgcccatgaa ggccgaatag aacgttttcg
     9601 acttcggctc cgcgccttcc ttcacgttct cgacgacgag ctcgagcgcg agcaccgcgc
     9661 cagtattggc gccgttgttg gcgatggtga tggggatcgc gaaaacgtcg acgtcgccgc
     9721 cgccgtcgcg cgcgtactgg agtaccggcg gtacatagac ctcgagctcg gcgcgttgca
     9781 gtgcactcat atagaagctg atccccgaga agacgaacgc cgccgccgag atcatggtcg
     9841 cggcccaggt gccgccgtgg ccagccgcct cgctcacgtg cgccattccg ccgtcgttgc
     9901 cgcccgtcat gaagatcccc tcgatgcctg ctgaaggtcc ggtcaaggtc tggccgtgca
     9961 atggggccat cgagtggcag cggatgccaa gcgcgagcat gcccgctaag ctccggtgtc
    10021 gcagcgaata cgcgaggagc gccatggccg aaaaattcgt gacgatcgag cgcgggctcg
    10081 gacctgaggg gcgcatcgcg ctcgtccgct tcgaccgggg cgacaggatc aacgcgctct
    10141 cgcccgaggc aatgcggcag ctgcgcgccg cggcgcaatc gttcgacgac gatctcgaaa
    10201 cgtccgtggt cgtgctgacg ggcaacgcgc atgccttctc cgccggcttc gacctgaagg
    10261 acgcggagcg caaggccaag gcggcgcaag ggcgcaagtc gatccgggtg ggaccggcca
    10321 tgtgcaaggc ctgggccgag atggagccgg tgacgatctg cgcgatcgag ggctttgcga
    10381 ttggcggcgg ggcagcactc gccgtctcgc tcgacttccg cattctgtct cgggcggcgc
    10441 acctgcgaat acccgagctg gcgctcggca tgaacatgag ctggggcacg atcccgcgcc
    10501 tcctccagct gatcggcccg gcgcgcacca agcaggcggt gatctgtgcc gaggaccgca
    10561 ttgaggccag ggaggcgctc gactggcggc tcgtcgagga gctcgccgat cccggcgcgg
    10621 cgctcgccgc cgccatg
//
LOCUS       DAORNH010000143        10584 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4667_length_10584_cov_2.669959, whole genome shotgun sequence.
ACCESSION   DAORNH010000143 DAORNH010000000
VERSION     DAORNH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10584
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4667_length_10584_cov_2.669959"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK264_14455"
     CDS             <1..196
                     /locus_tag="PK264_14455"
                     /inference="COORDINATES: protein motif:HMM:NF018067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HRD77109.1"
                     /translation="MVVFTLPSFPYVFKVIRDEILPPKETDRDTVKSIRKFQEAKGLK
                     VDGIIGRETLKKLFAPPATK"
     gene            complement(453..641)
                     /locus_tag="PK264_14460"
     CDS             complement(453..641)
                     /locus_tag="PK264_14460"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRD77110.1"
                     /translation="MLGLIFMSPKLGPLLMQVTAFTIAHSVTLALGIYGVMQVSPAIV
                     EPLKYYSKGDKGCISAAR"
     gene            651..962
                     /locus_tag="PK264_14465"
     CDS             651..962
                     /locus_tag="PK264_14465"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HRD77111.1"
                     /translation="MIEPLRQDMGKAEDGIFELMREGAAGLGASDQHGLAEFALLDPA
                     NGDEPTIRTVGLPLAIVAESNPHGLKAGESLPVRVLFRGKPIAGLPFKVFNLKGPKSP
                     R"
     gene            1194..2336
                     /locus_tag="PK264_14470"
     CDS             1194..2336
                     /locus_tag="PK264_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77112.1"
                     /translation="MSAITPRVGMKIYDNVWYLRHGLEPEQAADILAGMGINYVLTQS
                     KLLPMQDTAIESEVPAAERARFRRLDDLAFRRALAARGILYYHSILFGFDPSFLARHP
                     DLTPIDQHGRPAEKVDWYIGIPSERRSNTAHKIGALETVVRHLEPDGVHLGFIRWPGF
                     WEIWLDDVQRADMPDYCYAPATLSRFRAETGIDVPVHDARAAARLIAEQHKSAWSDWK
                     CGVTVATVGDVRRALAAIKPDIRIAINTLPFFLTDFDNAVEEVFGQSPARLSAVSDLF
                     EVMGYHQIMRRDASWPGAIAGDIKRRTGKTVVCTVQGSALYLDGIHAGRGRATTLDTD
                     EFILAVDGIERSEADGICVFTFTDFLDMRGTTDGERRIARLKAFRR"
     gene            2333..3199
                     /locus_tag="PK264_14475"
     CDS             2333..3199
                     /locus_tag="PK264_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRD77113.1"
                     /translation="MTASPGDSIGIDIGGTAIKAARVDGEGRILHAMTLPAGANLTAE
                     ALLALVRRALDDLSASGPVRRVGIAVAGVVRTDGLMPAGATNLPALADQHLPDLFSSV
                     LDRSASVINDAQAAMHGEAWSGAARGVANALMVTFGTGLGFGLLLDGRVHRGAHGASG
                     ELGAWVPHGAHGDLTIEAIAAPHRFEQRHGRRLGVHGFEHGADPETDAAIGAISRVLA
                     AAHLLLDLELIILAGGITAAGEPFRAVVEAAFRAATPGGLQHDVRLALSALGPYAGAV
                     GAVAPSVPETSA"
     gene            3196..3915
                     /locus_tag="PK264_14480"
     CDS             3196..3915
                     /locus_tag="PK264_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRD77114.1"
                     /translation="MNVIAARDYDDMSRRSAALVLDQLRAKPASVFVFPTGATPLGLF
                     RELVAAAKAGRADFSAASFAVLDEYAGLAPGDRRSLAGWLDRELIAPIGARAGQVITF
                     DPLGDGKAESGRVEAEIARRGGIDLAILGLGPNGHLGFNEPGTPFETRSGLVALAPAS
                     IASNSAYWGSEADVPRTGYTLGLGTLLGARKLGLIVSGAHKAAILKQTLAGPASIDLP
                     ATIIHVHSDATVIADAQALAG"
     gene            4155..4787
                     /locus_tag="PK264_14485"
     CDS             4155..4787
                     /locus_tag="PK264_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD77115.1"
                     /translation="MPRLKPDTQRARREHILDAAERCFARAGFHATTMQHICREAGVS
                     PGAFYVYFDSKEALIAGISERSRAELTERLIELGQAPDFIDALRMLGEKYFIDEPAHK
                     RLMCVEIGIEATRNRRVGEIFKETDSFVADSFERLFRRLKDDGRIAPELDIATLAKVF
                     LIIGDGMFWRRAVDPEFEPKSVMPAIAAVLAGLISPVPQMATAANKEMAP"
     gene            4784..6037
                     /locus_tag="PK264_14490"
     CDS             4784..6037
                     /locus_tag="PK264_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD77116.1"
                     /translation="MKRLALILLIAVGALAALAYSGHLPKPLADQVDRLLGKDRPKLL
                     ARAKGGPKTEIAPPIAVTVARAGKAKLTEAISATGSLVARTEIIVGPEIEGVRVVELY
                     ADEGDNVKKGQPLVRLVSDVLEAQLAQNDALRAKAEAAIAQARSQIAQAEARLAEAKS
                     AFDRARPLRSSGTISESIFEQREAAARTAEAQKAASLDGLKLAEAELAQIKAQRRELE
                     WRMSRTTVHSPADGVVARRLTKVGAMGVGAGEPMFRLISKGEIELEADVPEVQLGKIK
                     EGQTVRVSVAGVADVEGTIRLVAPEVDKSSRLGRVKVFLGSDPRLRVGAFARARIETA
                     QSEGLVVPTAAVLYSDDGAFVQLVVDSRIVTRKVKVGLASNGQTEITSGIAAGDTVVA
                     KSGTFLRDGDIVRPVVQTMTSASGG"
     gene            6041..>9159
                     /locus_tag="PK264_14495"
     CDS             6041..>9159
                     /locus_tag="PK264_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD77117.1"
                     /translation="MQLNVSAWAIRKPVPSLVLFLVLMVVGVFAFRQLPITRFPNIDV
                     PIVQVRVYQAGTAPTELEAQVTKKIEDAIAGVNGVKHMTSTVTEGASLTVIEFRLETN
                     ADRALNDVKDAIARVRSELPRTVEEPIVSRLDIEGLPIVTYGARAPGMTPEELSWFVD
                     DVVVRHLQGVKGVAAIERLGGVEREVRILLDPDRLAAYGITAGDVNRQVRATNIDLAG
                     GRGEIAGREQAIRTLAGKETVEGLAATMIALPRGRKVRLDELGTVTDGYAEPRTFATL
                     DKQPIVAFNITRSKGASDLVVAADVERAVAELAKRYPNVEMKIIDTTTTAVRGVYDST
                     MQTLIEGAVLAIIVVLIFLRDLRATIITAIALPLSIFPAYFMMDLMGFSLNLVSLLAI
                     TIATGILVDDAIVEIENIVRHMRMGKSAYKAALEAADEIGLAVIAITFTIIAVFTPVS
                     FMGGIAGQYFKQFGLVVAIAVFFSLLVARLVTPMLAAYFMRGAGHEEKETGWLRAYTR
                     LVRWSVRRRFTTVGIGLMIFAGSIGSFFLLPAAFIPPDDSGRILLAVELPPGSRLEDT
                     RRVTNNLIDRLKKRGDVESIIVNGGRVLGSGSEVRKATLIIRLVPKTERALSQIAITE
                     AISADIADVPDIRYWVLRDNGQRGFTVVVTGRDAAAVNRVAAELTSQSRRVTGKDGQP
                     LLLNVVSTAELDRPELRVVPKPDIAAELGVTTEAISEAVRIATIGDVGANLAKLTIDD
                     RQIPIRVQLAEKWRADRAILEDLKVVTATGASVPLSVVADFNLSQGATAIDRYDRQRR
                     VLIGGDLVGTTPLGLALTSVMALPAAKNLPAGVEIRQFGDAEIMKEVFESFAKAMGAG
                     IMMVYAVLVLLFGSFLQPITILFSLPLSIGGAIAALYVTGQPVSLPVVIGILMLMGIV
                     TKNAIMLADFAIEQMAKGMPRSEALVDAGRKRARPIIMTTIAMVGGMIPAAVNAGSGG
                     EFRVPMAVAVIGGLISSTALSLIFVPAVFAVMDDFGKGLWWLFGRFVGKGEEAELATA
                     AASSHGHGHA"
     assembly_gap    9160..9188
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9571..10293
                     /locus_tag="PK264_14500"
     CDS             9571..10293
                     /locus_tag="PK264_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD77118.1"
                     /translation="MPNAALLQNVDPRHIPAITAAFEPAKFAAGSQLFHEGDHGNDLF
                     LIDTGVVRLQQEREEIDGDAVLGFIDPGQIVGEVAVIDGGARSASAYAQTDVTARRLT
                     RAGLERLTSESPAAGAALLSALARVVAGKLRHTSDRLAAEIFTPTDPAIDALVARAVA
                     AQRVLAGLPDAQVDTALAGLSAAAAAAAEELLVFAAGAVKAPATCPTRSSRTALQRSA
                     STTRPSPDCRRRASSPRMRRTA"
     gene            10338..>10584
                     /locus_tag="PK264_14505"
     CDS             10338..>10584
                     /locus_tag="PK264_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77119.1"
                     /translation="MTNPAATAIFKALVCVKSRNALIMNFHRSTERLAVKTVGLFQKV
                     LMEHGLPADCIQSVQRRGSRKTTEMLMRSGLERAPVAG"
BASE COUNT         1723 a         3695 c         3431 g         1706 t
ORIGIN      
        1 gatggtggtt ttcacgctgc cgtcgttccc gtacgtcttc aaggtgatcc gggacgagat
       61 cctgccgccg aaggaaaccg accgcgacac ggtcaagtcc atccgcaaat tccaagaagc
      121 caagggcctc aaggtcgatg gcatcatcgg tcgcgaaaca ctcaagaagc tcttcgctcc
      181 gccggcgacg aaatagccca ctctggcagt gtgtctgcgt ctcgccctcc ctgctggcgc
      241 atgcccggat ccggcatgat gcaggcgagc ccgccggcag acatcggcac ctgcacgccc
      301 gtcttgccgg cgaggacgga ggcgcttcat gaggggctgg aagcgtaggc tgcatgaaac
      361 acggcgggcg gcgcgtcgcc ctgacccgcc gcccatggat gtcccggtca cagcctcggc
      421 gaaggcctcg cctgacgccg cgccggctcg agtcagcggg ccgcggagat gcagcccttg
      481 tcccccttgc tgtagtactt gagcggctcg acaatggccg gcgaaacctg catgaccccg
      541 tagatgccga gcgcaagcgt caccgagtgc gcaatcgtga acgcagtcac ctgcatcagg
      601 agcggcccca gtttcgggct catgaagatg agcccgagca cgaacaggat gtgatcgagc
      661 cccttcggca ggatatgggt aaggccgagg acggcatatt cgagctcatg cgcgagggtg
      721 ctgcgggcct aggcgcctcc gatcagcacg ggcttgctga attcgccctc cttgatccag
      781 ccaatggcga cgagccgacc attcgcacgg tcggtctccc cctcgcgatc gtcgccgaga
      841 gcaatcccca cggcctgaaa gccggcgagt cgctccctgt ccgcgtgctc ttcaggggca
      901 agccgattgc cgggctgccc ttcaaggtgt tcaacctgaa aggtccgaag tcgccgcgat
      961 aggcggtctt cgatccggat ggccgggtct ccatcgagtt gccggtgcga ggcgaatacc
     1021 tcgtgcacac cgtgcacatg ttcgagccgg aggcaggttt ggccgcgcac tggtcgagcc
     1081 cgtgagcctc gctgacgatt tttccgccga aggcgaccgg cccgtgaggt gcccgtttga
     1141 gcttcaccct gccgccgcgc tagtctccct gctcgccgga ggagtccgcc cacatgtccg
     1201 ccatcacgcc gcgcgtcggc atgaagatct acgacaacgt ctggtatctg cgtcacggcc
     1261 tcgagccgga gcaggcggcg gatatcctcg ccggcatggg catcaactac gtgctgaccc
     1321 agagcaagct cttgcccatg caggacaccg ccattgagag cgaggtcccg gccgccgagc
     1381 gcgcgcggtt ccgaagactc gacgatctcg ccttccgccg tgcgctcgcc gcgcgcggca
     1441 tcctctacta tcactcgatc ctgttcggct ttgatccgag ttttctcgcg cgccaccccg
     1501 acctgacgcc gatcgaccag catggacgcc cggccgaaaa ggtcgactgg tacatcggca
     1561 tccccagcga gcgacggagc aacaccgccc acaagatcgg cgcgctcgag acggtcgtcc
     1621 gccacctcga gccggacggc gtgcacctcg gcttcatccg ctggccgggc ttctgggaga
     1681 tctggctcga cgacgtgcag cgcgccgaca tgcccgacta ctgttatgcc cctgcgaccc
     1741 tctcccgctt ccgcgccgaa accgggatcg acgtccccgt gcatgacgcg cgcgccgcgg
     1801 cccgtctcat cgctgagcag cacaagtccg cctggagcga ctggaaatgc ggcgtgaccg
     1861 tcgcgactgt tggcgacgtg cgacgcgcgc tcgcagcgat caagcccgac atccggatcg
     1921 ccatcaacac gctgcctttc ttcctcacgg atttcgacaa cgctgtcgag gaggtattcg
     1981 gccagagccc cgcgcgcctc tcggccgtct ccgacctctt cgaggtgatg ggctatcacc
     2041 agatcatgcg ccgggacgcg agctggcccg gggccatcgc gggcgacatc aagcggcgca
     2101 cgggcaagac ggtggtgtgc acggttcaag gcagcgcgct ctatctcgac ggcatacacg
     2161 ccggccgcgg tcgtgcgacc accctcgaca ccgacgagtt cattcttgcc gtcgacggca
     2221 tcgagcggag cgaggccgac ggcatctgcg tcttcacatt caccgatttt ctcgacatgc
     2281 gcggtaccac ggacggtgag cggcgtatcg cgcggctgaa ggccttccgg cgatgaccgc
     2341 gtcgccggga gacagcatcg gcatcgacat cggcggcacg gcgatcaagg ccgcgcgcgt
     2401 cgacggcgag ggtcgcatac tccatgcaat gaccctcccg gccggcgcaa atttgaccgc
     2461 agaggcgctg ctcgccctcg tgcgccgcgc gctcgacgat ctctcagcga gcggacctgt
     2521 ccgccgggtc ggcatcgccg tcgccggtgt ggtgcggacg gacggtctca tgccggccgg
     2581 agcgacaaac ttgccggcgc tcgcggatca gcacctgccg gacctcttct cctcggtcct
     2641 cgaccggtcg gcgagcgtca tcaacgacgc ccaggccgcc atgcacggcg aagcctggtc
     2701 gggcgcggcg cgtggcgtcg cgaacgcgct gatggtgacc ttcggcacag ggctcggttt
     2761 cggactgctc ctcgacggcc gcgtccaccg tggcgcgcac ggggcctcgg gtgagctcgg
     2821 cgcctgggtg ccgcatggcg ctcacgggga tctgaccatc gaggcgatcg ccgcgccgca
     2881 ccgcttcgag cagcggcatg gccggcgcct cggcgtacat ggcttcgagc acggcgcgga
     2941 ccccgagacg gacgccgcca tcggcgcgat cagccgtgtg ctcgcggccg cgcacctcct
     3001 ccttgatctc gagctgatca tactcgcggg cggtataacc gccgcaggcg agccgtttcg
     3061 cgcggtcgtc gaggccgctt tccgcgccgc cacgccgggc ggcctccagc acgatgtccg
     3121 ccttgccctt agtgcactcg gaccgtatgc cggtgccgtc ggcgccgtcg ccccatcagt
     3181 tccggagacc tcggcttgaa cgtcatcgcc gctcgcgact acgacgacat gagccgacgc
     3241 tcggccgccc tcgtgctcga tcagcttcgt gccaagcccg cctcggtgtt cgtcttcccg
     3301 acgggggcga cgccgctcgg cctcttccgc gagttggtcg cagcggccaa ggcgggccgc
     3361 gccgatttct ccgctgcatc cttcgccgtg ctcgacgagt acgcgggcct cgcgcccggc
     3421 gatcgccgca gcctcgccgg ctggctcgac cgcgagctga tcgcccccat cggagcccgt
     3481 gccggtcagg tgataacctt cgatcccctc ggcgatggca aggcggaaag cggccgggtg
     3541 gaagcagaga tcgcgcgccg tggcggcatc gacctcgcca tcctggggct cgggccgaac
     3601 gggcatctcg gcttcaacga gccgggcacg ccgttcgaga cgcggtccgg gctcgtggca
     3661 ctcgcgccag cgtcgattgc ctccaactcc gcatattggg gcagcgaggc ggacgtgccg
     3721 cgcaccggat acacgctcgg tctcggcacg ctgctcggcg cgcgcaagct cgggctgatc
     3781 gtgagcggag cgcacaaggc tgcgatcctg aagcagacgc tcgccgggcc ggcgtcgatc
     3841 gacctgcctg ccacgatcat ccacgtgcat tcggacgcaa cagtgatcgc cgacgcacag
     3901 gcgttggccg gctgaccaga gaggtcgcac cccgagtcgt ttggggcggt gccccaaacc
     3961 ccgccgaaga cctcggcctt tggaaaccgt tcttagggtc tcgggcgtct ctgatccttt
     4021 cgcgcagacc acacgtaaaa ctcgttcatg cggcgcgtac ccatcagatt tcgttgaaac
     4081 atcagctcgc ctgttgacaa gtgagggcgt ttcagcaaat aaaacgatcg ttcgttttgt
     4141 gatcggaaac tcctatgccc agactgaaac cagacacgca gcgggcaagg cgcgagcaca
     4201 tcctcgatgc cgcggagcgc tgtttcgcgc gcgcggggtt ccatgcgacc accatgcagc
     4261 atatctgcag ggaggcgggg gtcagcccgg gcgccttcta cgtctatttc gacagcaaag
     4321 aggcgctgat cgccggcatc tcggagcgca gccgcgccga gctcaccgag cggctgatcg
     4381 agctcggcca ggcacccgat ttcatcgacg cgctgcgtat gctcggcgag aagtatttca
     4441 tcgacgagcc ggcacacaag cggctcatgt gcgtcgagat cggcatcgag gcgacgcgca
     4501 accgccgcgt cggtgagatc ttcaaggaga ccgacagctt cgtcgccgac agcttcgaaa
     4561 ggctgttccg acgtctcaag gacgatggcc gcatcgcacc agaactcgac atcgccacgc
     4621 tcgccaaggt gttcctgatc atcggcgacg gcatgttctg gcgtcgcgcc gtcgatcccg
     4681 aattcgaacc caagtccgtc atgccggcca tcgccgcggt gctcgccggt ctcatcagcc
     4741 ccgttccgca gatggccacc gctgcaaaca aggaaatggc gccatgaagc gtctcgccct
     4801 catcctgctg attgccgtcg gcgcgctcgc cgcgctcgcc tactcgggcc atttgccgaa
     4861 gccgctcgcc gatcaggtcg atcgcctcct cggcaaggac cggccgaagc tgctcgccag
     4921 ggcaaagggt ggcccgaaga cggagatcgc gccgccgatc gccgtgacgg tcgctcgcgc
     4981 cgggaaggca aagctcaccg aggcgatcag cgccaccggc tccctcgtcg cgcgcaccga
     5041 gatcatcgtc ggtcccgaga tcgagggcgt gcgcgtcgtc gagctctatg ccgatgaagg
     5101 cgacaacgtg aagaagggcc agccgctggt ccgcctcgtc agcgacgtcc tcgaggcgca
     5161 gctcgcccag aacgatgcgc tccgcgccaa ggccgaggcg gcaatcgccc aggcgagaag
     5221 ccagatcgcg caggccgagg cacggctcgc tgaagcgaag tcggcattcg accgcgcccg
     5281 cccgctgcgc agctccggca ccatctccga gagcatcttc gagcagcgcg aagccgctgc
     5341 tcgcaccgcc gaggctcaga aggccgcatc actcgacggc ttgaagctcg ccgaggcgga
     5401 actcgcgcag atcaaggcgc agcgccgcga gctcgaatgg cgcatgtcgc gcacgacggt
     5461 ccattcgccg gccgacggtg tcgtcgcccg ccgcctgaca aaggtcggcg ccatgggcgt
     5521 cggcgcgggc gagcccatgt tccgcctgat ctccaagggc gagatcgagc tcgaggcgga
     5581 tgtgcccgag gtgcaactcg gcaagatcaa ggaaggtcag accgtgcgcg tgagcgttgc
     5641 cggcgttgcc gacgtcgaag gcacgatccg cctcgtcgcg cccgaggtcg acaagtcgag
     5701 ccggctcggt cgcgtcaagg tgttcctcgg ctcggacccg cggctccgcg tcggcgcctt
     5761 cgcgcgcgcc cgcatcgaga ccgcccagtc cgaggggctg gtggtaccga cggccgctgt
     5821 tctctattcg gatgacggcg cattcgtgca actcgtcgtc gacagccgca tcgtgacacg
     5881 caaggtgaaa gtcgggctcg cctccaacgg tcagaccgaa atcacatccg ggatcgccgc
     5941 tggcgacaca gtcgtcgcca agtcgggcac attcctgcgc gacggcgaca tagtccgccc
     6001 tgttgtgcag accatgacct cggcgagcgg gggctgagcc atgcagctga atgtttccgc
     6061 ctgggccatc aggaagcctg tcccatcgct cgttctcttc ctcgtgctga tggtggtcgg
     6121 cgtgttcgcc ttcaggcagc tgccgatcac ccgcttcccg aacatcgacg tgccgatcgt
     6181 ccaggtgcgc gtctatcagg ccggcaccgc gccgaccgag ctcgaggcgc aggtcaccaa
     6241 gaagatcgag gacgccatcg ccggcgtgaa cggcgtgaag cacatgacct cgaccgtcac
     6301 ggagggcgcc tcgctcaccg tgatcgagtt ccgcctagag accaacgccg accgcgcgct
     6361 gaacgatgtc aaggacgcga ttgcccgcgt ccgctcggag ctgccacgca ccgtcgaaga
     6421 gccgatcgtc tcgcgcctcg acatcgaggg actgccgatc gtcacctacg gcgcgcgcgc
     6481 ccccggcatg acgccggagg agctctcctg gttcgtcgac gacgtggtcg tccgccatct
     6541 acagggtgtg aagggcgtcg ccgcgatcga acgtctcggc ggcgtcgagc gcgaggtgcg
     6601 cattctcctc gaccccgacc ggctcgccgc gtacggcatt actgcgggcg acgtgaaccg
     6661 gcaggtgcgc gcgaccaaca tcgacctcgc gggcggacgc ggcgagatcg cgggccgcga
     6721 gcaggcgatc cgcactcttg ccggcaagga gaccgtcgaa ggcctcgccg cgaccatgat
     6781 cgcgctgccg cgtggtcgca aggtccggct cgacgaactc ggcacggtca ccgatggcta
     6841 cgccgagccg cgcacctttg cgaccctcga caagcagccg atcgtcgcct tcaacatcac
     6901 gcgctcgaag ggggcgagcg atctcgtcgt cgcggccgac gtcgaaaggg cggtcgccga
     6961 gctcgcgaag cgctacccga acgtcgagat gaagatcatc gacacgacga cgacggctgt
     7021 ccggggcgtc tacgattcca caatgcagac gctgatcgag ggcgccgtgc tggcgatcat
     7081 cgtcgtgctg atcttcctgc gcgacctgcg cgccaccatc atcacggcga tcgcgctgcc
     7141 gctgtccatc ttcccggcct acttcatgat ggacctgatg ggcttctcgc tcaacctcgt
     7201 gagcctgctc gccatcacga tcgcgacagg catcctcgtc gacgacgcca tcgtcgagat
     7261 cgagaacatc gtgcgccaca tgcgcatggg gaaatccgcc tacaaggcgg cgctcgaggc
     7321 cgccgacgag atcggcctcg ccgtgatcgc catcaccttc acgatcatcg cggtgttcac
     7381 accggtgagt ttcatgggcg gcatcgcggg gcagtatttc aagcagttcg gtctcgtcgt
     7441 cgcgattgcc gtgttcttct cgctgctcgt cgcccggctc gtcacgccga tgctcgccgc
     7501 ctacttcatg cgcggcgccg gccacgagga gaaggagacc gggtggctgc gcgcctacac
     7561 acgcctcgtg cgctggtccg tccgccgccg cttcacgacg gtcggcatcg gcctcatgat
     7621 cttcgcaggc tcgatcggca gcttcttcct gctgccggcg gcgttcatcc cgccggacga
     7681 ctcgggccgc atcctgctcg ccgtggagct gccgccgggt tccaggctcg aggacacgcg
     7741 ccgcgtcacc aacaatctca tcgatcggct caagaagcgc ggtgacgtcg agagcatcat
     7801 cgtcaatggc ggccgcgtgc tcggctccgg ttccgaggtg cgcaaggcga cgctgatcat
     7861 ccgcctcgtg ccgaagacgg agcgggcgct gagccagatc gccatcaccg aagcgatcag
     7921 cgccgacatc gccgacgttc ccgacatccg ttactgggtg ctgcgcgaca acggccagcg
     7981 cggcttcact gtggtcgtca ccgggcgtga cgcggcggcc gtcaaccggg tcgccgccga
     8041 gctgacgagc caatcgcgcc gcgtcacggg gaaggacggg cagccgctgc tgctcaacgt
     8101 cgtctccacc gccgagctcg accgtcccga gctgcgggtc gtccccaagc cagacatcgc
     8161 cgccgagctc ggggtgacga ccgaggcgat ctcggaggct gtgcgcatcg caacgatcgg
     8221 cgacgtcggt gctaacctcg ccaagctcac catcgacgac cgccagatcc cgatccgcgt
     8281 gcagctcgcc gaaaagtggc gcgccgaccg cgccatcctc gaggacctga aggtcgtgac
     8341 cgcgacaggg gcatccgtgc cgctctccgt cgttgccgac tttaatctga gccagggtgc
     8401 gacggccatc gaccgctacg accggcagcg gcgcgtgctg atcggcggcg acctcgtcgg
     8461 cacgaccccg ctcggcctcg ccctcacgtc ggtgatggca ttgccggccg cgaagaacct
     8521 gccggccggc gtcgagatcc gccagttcgg cgatgccgag atcatgaagg aggtgttcga
     8581 gagcttcgcg aaggccatgg gcgccggcat catgatggtg tacgccgtcc tcgtcctgct
     8641 gttcggctcg ttcctgcagc cgatcacgat cctcttctcg ctgccgctgt cgatcggcgg
     8701 cgcgatcgcc gcgctctacg tcaccgggca gccggtgagc ctgcctgtgg tgatcggcat
     8761 cctcatgctg atgggcatcg tcacgaagaa cgcgatcatg ctcgccgatt tcgcgatcga
     8821 gcagatggcg aagggtatgc cccgttccga agccctcgtc gatgccggcc gaaagcgcgc
     8881 ccggccgatc atcatgacga ccatcgccat ggtcggcggc atgatcccgg ccgccgtcaa
     8941 cgcgggttcg ggcggcgaat tccgcgtacc gatggctgtc gctgtgatcg gcggcctgat
     9001 ctcctcgaca gcgctgtcgc tgatcttcgt gccggccgtg ttcgcggtga tggacgattt
     9061 cggcaagggc ctgtggtggc tgttcggccg cttcgtcgga aagggtgagg aggcggagct
     9121 cgcgaccgcg gcggccagca gccacggaca cggacacgcn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnct tccccgccac gggtcgtgtg gagcactcgg aagcgccgac cgggccgaaa
     9241 atgagcacat cagcggccac ccctgcggcg gcaatcgccg cagctgccgt cgccagagtt
     9301 gaagcgcccg cccagccggc cccgtcaacg ccgggacagg aggagccggc tcccccgcgc
     9361 cctctcgccg ccgagtaggg ccgcgggagg tgttgcggca cccactcggg cgtcaggcgt
     9421 cgccaaggct tgcgggtcgc ccccggagcg agtgcgcggc cgctggcaac cttgcatgat
     9481 gtggcggcgt gcagcgccag tgggcatctg gtaggctcgg tccaagtccg agccgggctt
     9541 ttccggcatg cagccgattg cgaggccccc gtgcccaatg ccgctcttct ccagaatgtc
     9601 gatccgcgcc atatcccggc cataacggcc gccttcgagc ccgcgaagtt tgcggcagga
     9661 agccagctct ttcacgaggg cgaccatggc aatgacctat tcctcatcga caccggcgtc
     9721 gtgcgactcc agcaggaacg cgaggagatc gacggcgatg ccgtgctcgg cttcatcgat
     9781 ccgggccaga ttgtcggcga ggtcgcggta atcgacggcg gcgcgcgctc agccagcgcc
     9841 tacgcacaga cggacgtgac ggcgcgccgg ctgacgcggg ccggcctcga gcggctgacg
     9901 tccgaaagcc ctgcggcagg tgccgccctt ctcagcgcgc tcgcccgcgt ggtggcgggc
     9961 aagctgcgcc acacaagcga tcggctggcc gccgagatct tcacgccgac tgatccggca
    10021 atcgatgccc tcgtcgcacg tgcggttgcc gcacagcgcg tcctcgccgg gctgccggat
    10081 gcccaggtgg atacggcact ggccgggctc tccgctgctg ccgccgcggc ggccgaggag
    10141 cttctggtgt tcgcggcagg tgcggtcaaa gcgccggcaa cgtgcccgac aagatcgtca
    10201 agaaccgcat tgcagcgctc ggcgtctacc acaagaccat cgccggattg ccggcgacgg
    10261 gcgtcatcgc cgagaatgcg gcgcacggcc tgaccgagct cgcggccccc gtcggtgtcg
    10321 tcttcggact gatcccgatg accaatccag ccgccacggc gatcttcaag gcgctggtgt
    10381 gcgtgaagag ccgcaatgcg ctgatcatga acttccaccg cagcaccgaa cggctcgcgg
    10441 tgaagaccgt cgggctgttt cagaaggtgc tcatggagca cggactgccg gccgactgca
    10501 tccagtccgt gcagcgtcgc ggctcgcgca agaccaccga gatgctgatg cgctcggggc
    10561 tcgagcgggc gcccgtcgcc ggct
//
LOCUS       DAORNH010000144        10528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4702_length_10528_cov_2.529552, whole genome shotgun sequence.
ACCESSION   DAORNH010000144 DAORNH010000000
VERSION     DAORNH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4702_length_10528_cov_2.529552"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK264_14510"
     CDS             <1..350
                     /locus_tag="PK264_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77120.1"
                     /translation="REHWRVAVTGATLSIEDSIFFAQASGPRPTRQIVLRGICAGETE
                     VSWAFEMLGDTGSDVQLAAALASIETAILGLPDEPVVPPPLPSDGAGAPDMLGGEKAS
                     GPPPLPDPNKDKS"
     gene            347..1939
                     /gene="purH"
                     /locus_tag="PK264_14515"
     CDS             347..1939
                     /gene="purH"
                     /locus_tag="PK264_14515"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRD77121.1"
                     /translation="MTATAAPRIRRALISVSDKAGIVDFARSLAAGGVEVISTGGTAV
                     ELKTAGIPVTDVAVHTGFPEMMDGRVKTLHPKVHGGLLAIRGNAGHEASAREHQIPPI
                     DLLVVSLYPFEATVAKGAAFDDCVENIDIGGPAMIRSAAKNHASVTVIVDPADYGRVR
                     DEMRANAGATTAALRRALALKAFARTAAYDAAISTWLAGEIGEREPKWMSFAGERSGS
                     LRYGENPHQTAAFYRTGARRPGVATLVQHQGKELSYNNLNDTDAAFELVAEFDPAMPA
                     VAIIKHANPCGVAVGASARDAYLAALRCDPVSAFGGIVALNCPIDGEAAAEIAKIFTE
                     VVIAPEATAEAKAIFAVKKNLRLLTTGGLPDPRAWGLMVRSLAGGLLVQSRDNAVVDD
                     MSLKAVTKRQPTPAELADLKFAFRVAKHVKSNAIVYVKGGATVGIGAGQMSRVDSSRI
                     AAWKAGEAAKAAGLAESLAKGSVVASDAFFPFADGLLAAAEAGATAVIQPGGSMRDDD
                     VIKAADERGLAMVLTGVRHFRH"
     assembly_gap    2082..2092
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2323..>2608)
                     /locus_tag="PK264_14520"
     CDS             complement(2323..>2608)
                     /locus_tag="PK264_14520"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD77122.1"
                     /translation="TASAAWPHDQKGERWQSRIPVNGKQVPTTDQLFWAGYSGVLYLP
                     STVGPVGLTAKSKLPVGYQAIARHGHDKTAIAFSRAVEREIGGFVPPPGY"
     assembly_gap    2609..2788
                     /estimated_length=180
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2789..3779)
                     /locus_tag="PK264_14525"
     CDS             complement(<2789..3779)
                     /locus_tag="PK264_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD77123.1"
                     /translation="METPLHFKSAVEIAALIRSKKLSAVDALDHFLKRVATHNPKLNA
                     IIWMDAERARKRAKAADAALAKGEVWGPLLGVPMTIKESYQVTGSPTTWGDPANKNNV
                     TETSALAVTRLEKAGVVLFGKTNVPLMLADWQSYNAIYGTTNNPWNTAMIPGGSSGGS
                     AAALAAGLTGVEAGSDIGSSIRNPAHYCGVFGLKPTYGVIAMRRHSLPGAFAPADISA
                     IGPLSRGAEDLDVMLDAMSGPDELDDQCWKLELPKARATAIKDMRIAVKLRDPNCEVD
                     GEYADRLQNLVDHLARKGAKVKEIEPAIDTRRLHEIYVLLLRAATSGRTPLADI"
     gene            complement(3789..4775)
                     /locus_tag="PK264_14530"
     CDS             complement(3789..4775)
                     /locus_tag="PK264_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77124.1"
                     /translation="MKVLIVGGAGMVGRKLAERLARDGTLGGKAISQLALYDVVAAEK
                     PAGAKFPVEISTGDLPAAGETDKLVAGKPDVIFHLAAIVSGEAEQDFDKGYRINLAGT
                     MNLLDSIRRAYDGAARKPRLVFTSSIAVFGAPFPEKIGDEFFTTPLTSYGTQKAIGEL
                     LLADYTRKGLLDGVGIRLPTICVRPGKPNKAASGFFSNIMREPLAGQEAVLPVSDDVM
                     HWHASPRAAVGFLLHAATIDGETMGPRRNLSMPGVAVTVGGQIEALRKVAGEKVVKRI
                     RREPDPFVQKIVAGWPRNFETKRAISLGFKADASFEEIVKIHIEDELKGKIA"
     assembly_gap    4846..4855
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5446..6396
                     /locus_tag="PK264_14535"
     CDS             5446..6396
                     /locus_tag="PK264_14535"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRD77125.1"
                     /translation="MSSHSILQSRLAGAQLALTLRIGLGLVFVIGGWAKLERLLTPSR
                     AKGIVDEYVGPLGYINQTFLDWLFQSPLGVVATPWNFLTLLSAFELVSGIVLIAGLMV
                     RPLAVFWALLLWSFVFSLPVVTTPGVKPPAATYMSPAEFVQIRDIALSGFFFVLYNLG
                     PGARSVDHDVFAVPAERPHDWEALGLLLRLSLGVVFLVGGLFAGYNKITTFGMPGLLL
                     TLIGAGLVAGLWTRTFGAAAAALLVYFMVSKLAGASGIVGYLNSVKREFALLAGAGVL
                     ALAGGGSRFAATRAIQSFNEWTHIYVGGQRVSEAIPARQK"
     assembly_gap    6447..6543
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6544..6866
                     /locus_tag="PK264_14540"
     CDS             <6544..6866
                     /locus_tag="PK264_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77126.1"
                     /translation="WSLVWFLVGGWVSTVAQILVIVGVIFLYKYGWQRAPLEIWSRSR
                     NLARFVWAWARQRELPAAENRRGEAARDVVRVVKVKEFGDVNLSTLLSLVMIGGIVLL
                     STGF"
     gene            6879..>7427
                     /locus_tag="PK264_14545"
     CDS             6879..>7427
                     /locus_tag="PK264_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD77127.1"
                     /translation="MTGPLDGKVAIVTGSARNIGRETASALAAMGAAVMLHAKSDRDG
                     LEEAKALLAARGARVAGHLADLSTEAGANGLVAAAVADLGRVDILINNAAIRRNTSFT
                     TITLAEWREVLGASLDAAFLTSRAAVPHMRGGKWGRIVNVGGITMFKGTPGRAHVAAA
                     KAGMMGMTRVLASELAPDGITVN"
     assembly_gap    7428..7437
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7438..7644
                     /locus_tag="PK264_14550"
     CDS             <7438..7644
                     /locus_tag="PK264_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77128.1"
                     /translation="MISPGIIDTKRGAAAGARPAATQTLAGVPLGREGTTAEIAHVVA
                     MLCHPNGGYTTGQVLEVNGGMHFS"
     gene            7950..8363
                     /locus_tag="PK264_14555"
     CDS             7950..8363
                     /locus_tag="PK264_14555"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRD77129.1"
                     /translation="MAFKAKYPIGAIVVVNDDRRLFYVLGDGRAIRYPIAVGSEDNLW
                     TGQSFVQSKAKDPKWIPPWEPEREVEAGPDNPLGPRAIYLGWTLYRIHGTNSPRSIGG
                     AASSGCFRMYNHHAKDLYERVHIGAPVYVVDSLDD"
     gene            8633..9346
                     /locus_tag="PK264_14560"
     CDS             8633..9346
                     /locus_tag="PK264_14560"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRD77130.1"
                     /translation="MIGRARVGFIALAFAAAAMISIPGEAKAQSGDWLWGGSFVSGGS
                     GREDVRLNTTYGRGTIIVSFSDRRLYLVTGPGTAISYPIAIPRDQSRWQGTTNVSMKK
                     INPPWVPTATMRAENPRLPNFVPGGHPLNPLGVRALYLGSSAYRIHGTDAPWTIGQNV
                     SKGCVRMYNQDVIDLYNRVPVGTRVIVTWQRFQAGAPMVSMAPISDDEARPTRRYSRS
                     TVPTRRAVRTKSASAAAAE"
     gene            9661..>10528
                     /locus_tag="PK264_14565"
     CDS             9661..>10528
                     /locus_tag="PK264_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HRD77131.1"
                     /translation="MPKSSPSLNDKFDLDEHWQLLNGSQAIVRLTLMQRARDVKAGLN
                     TAGFVSGYRGSPIAGLEGAFQRAEAITRENHIRFHPGLNEDLAATALWGAQQVGVIKE
                     EAKYDGVFGIWYGKGPGVDRTGDVFRHANHAGTAKHGGVLALTGDDHTCESSTSAHQS
                     EFAMVDYMIPVFNPAGVQELLDYGIYGFALSRYAGVWVALKCVKDNFESTATVDGRLD
                     RVQVVIPGEDVLRLPSDGLNIKPAMADTALAKEQRLHDYKRQAMLAFARANKLDKLVI
                     SGGDAPKIGIITT"
BASE COUNT         1635 a         3311 c         3507 g         1767 t
ORIGIN      
        1 cgcgcgagca ctggcgggtc gccgtgacag gcgccacatt gtcgatcgag gacagcatct
       61 tcttcgccca ggcgagcggg ccgcgaccga cgcgccagat cgtgttgcgc ggcatctgcg
      121 cgggcgagac cgaagtgtcg tgggccttcg agatgctcgg cgacacgggc tccgacgtgc
      181 aactcgcggc ggcacttgcc agtatcgaga cggccatcct cggtttgccg gacgagcccg
      241 tcgtgccgcc accgcttccg tcggatggag caggtgcccc ggacatgcta ggcggcgaga
      301 aagcgagcgg tccgccgccg cttccagatc cgaacaagga caagtcatga cagcgacggc
      361 tgctccgcgc atcaggcgcg ccctcatctc agtctcggac aaggccggga tcgtcgactt
      421 tgcaaggtca ctcgcggccg gtggtgtcga ggtcatttcg accggaggaa ccgctgtcga
      481 gctcaagacg gccggaattc cagtgacgga cgttgccgtg catacaggct tccccgagat
      541 gatggatggc cgcgtcaaga cgctgcatcc caaggtgcac ggcggcctgc tcgccatccg
      601 cggcaatgct ggtcacgagg cctcggcgcg cgagcatcag atcccgccga tcgacctgct
      661 ggtcgtctcg ctctatccct tcgaggcgac ggtcgctaag ggcgccgcat tcgatgactg
      721 tgtcgagaac atcgacatcg gaggcccggc gatgatccgc tcggccgcga agaaccacgc
      781 gtctgtgacg gtcatcgtcg atccggctga ctacggccgc gttcgcgacg agatgcgggc
      841 caatgccggc gctaccaccg cggcgctccg gcgcgccctc gccctgaagg ccttcgcgcg
      901 caccgcggcc tatgacgccg cgatctcgac ctggctcgcc ggcgagatcg gcgagcgcga
      961 gcccaaatgg atgagctttg ccggcgagcg gagcggctcg ctgcgctatg gcgagaaccc
     1021 gcatcagacg gcggccttct accggacggg cgcgcgccgg ccgggggtcg cgacactcgt
     1081 ccagcaccag gggaaggagc tctcctataa caacctgaac gatacggacg cggccttcga
     1141 gcttgttgcc gaattcgatc ccgcgatgcc ggcggtcgct atcatcaagc acgcgaaccc
     1201 gtgcggtgtc gcggtcgggg cgagcgcgag agatgcctac ctcgcagcgc tcagatgcga
     1261 tccggtgagc gcgttcggcg gtatcgtcgc gctcaattgc ccgatcgacg gggaggcggc
     1321 ggccgagatc gccaagatct tcaccgaggt cgtgatcgca cccgaggcaa cggccgaggc
     1381 gaaggcgatc tttgccgtga agaagaacct gcgcctgctc accacgggtg gcctccccga
     1441 tccgcgcgcc tgggggctca tggtgcgctc tctcgccggc ggtctcctcg tccagtcgcg
     1501 cgacaacgcc gtcgtcgacg acatgagcct caaggcggtg acgaagcgcc agccgacgcc
     1561 ggccgaactc gccgacctca agttcgcgtt ccgcgtggcc aagcatgtga agtccaacgc
     1621 catcgtctac gtgaagggcg gcgcgacggt cggcataggc gccgggcaga tgagccgcgt
     1681 tgacagctct cggatcgcgg cctggaaagc cggcgaagct gccaaggccg caggcctcgc
     1741 ggagagcctc gccaagggct ccgtcgtcgc ctccgatgcg ttcttcccgt ttgccgacgg
     1801 tctgctcgcg gcggccgagg caggggcgac ggctgtgatc cagccgggcg gctcgatgcg
     1861 cgacgacgac gtgatcaagg ccgccgacga gcgcggcctc gccatggtgc tgacgggcgt
     1921 ccgccatttc cgtcactgac gccggtgagg tcacgtccat cgccgacatg caggagcgtg
     1981 actctcatag ccgtggcggg taggggcagg gctcgcttcg cgaacgaggc tgccgcgtcg
     2041 tgttgggagc ccgcctcagg cgcgacgtgc tccggtcgga gnnnnnnnnn nncccctttt
     2101 gggacctcca gaagggccgg gtctggggcg gcaagcccag gcgggtctgg acccgcggcc
     2161 cagtcgcggt gcgagccggc cggaccgctc gtcccacgtc cgtcattctg gcgaaggcca
     2221 ggacccaggt ccccgggcga ggggactgtt gcgggcggct ccgaggtgac ctggggcccg
     2281 cacttcggcg gcataccggc gcgcgggcgc gccggggcgc catcaatacc cgggcggggg
     2341 gacgaagccg ccgatctcgc gctcgacggc acgcgagaag gcgatcgcgg tcttgtcgtg
     2401 gccgtgccgg gcgatcgcct gatagccgac tggcagcttc gatttcgccg tgaggccgac
     2461 cgggccaacg gtcgagggca gatagagcac gcccgagtag ccggcccaga agagctggtc
     2521 cgtggtcggc acctgcttgc cgttgacggg gatgcgactc tgccagcgct cgcccttttg
     2581 atcgtgcggc caggcggcag aggccgtcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg atgtcggcga gtggcgtgcg cccggaggtg
     2821 gccgcgcgaa gcaacaggac gtagatctcg tggaggcggc gcgtatcgat ggccggctcg
     2881 atctccttca ccttggcgcc cttgcgggct aggtgatcga cgaggttctg caggcgatcc
     2941 gcgtattcgc cgtcgacctc gcagttggga tcgcggagct tcacggcgat ccgcatatcc
     3001 ttgatcgcgg tcgcgcgcgc cttggggagc tcgagtttcc agcactggtc gtcgagctcg
     3061 tcggggccag acatggcgtc gagcatgacg tcgaggtcct cggcaccacg cgagagcggg
     3121 ccgatcgccg agatgtcggc gggcgcgaag gcaccgggta gcgaatggcg gcgcatggcg
     3181 atcacgccgt aggtgggctt caggccgaag acaccgcagt agtgcgccgg gttgcggatc
     3241 gaggagccga tgtcggagcc ggcctcgacg ccggtgaggc cggccgccag cgccgcagcc
     3301 gacccgccgg acgatccgcc cgggatcatc gccgtgttcc agggattatt ggtcgtgccg
     3361 tagatggcgt tgtagctctg ccaatcggcg agcatcagcg gcacgttggt tttgccaaag
     3421 aggacgacgc ccgccttttc gaggcgcgtc acggcgagcg cgctcgtctc ggtcacgttg
     3481 ttcttgttcg cgggatcgcc ccaggtcgtc ggcgagccag tgacctgata gctctccttg
     3541 atggtcatcg gcacgccgag gagcggtccc cagacctcgc ccttggccaa tgcggcgtcg
     3601 gcggccttcg cgcgcttgcg cgcccgctct gcatccatcc atatgatagc gttgagcttg
     3661 ggattgtgcg tggcgacgcg cttcaggaag tggtcgaggg cgtcgacggc cgagagtttc
     3721 ttcgagcgga tgagggccgc gatctcgacg gccgacttga aatgcaacgg cgtttccatg
     3781 gggccccctt aggcgatctt tcccttgagc tcgtcctcga tgtggatctt cacgatttcc
     3841 tcgaaggagg cgtcggcctt gaaaccgagc gagatcgcgc gcttcgtctc gaaattgcgc
     3901 ggccagccgg cgacgatctt ctggacgaac gggtcgggct cgcggcggat gcgtttcacg
     3961 accttctcgc cggcgacctt tcgtagggcc tcgatctggc cgccgaccgt gacggcgacg
     4021 cccggcatcg aaagattgcg gcgcgggccc atcgtctcgc cgtcgatggt cgctgcgtgc
     4081 agcagaaagc cgacagcggc ccggggcgag gcatgccagt gcatgacgtc gtcactgacc
     4141 ggaagcaccg cctcctggcc ggcgagcggc tcgcgcatga tgttcgagaa gaagcccgag
     4201 gccgccttgt tgggtttgcc cgggcggacg cagatggtcg gcaggcggat gccgacgccg
     4261 tcgagcaggc ccttgcgcgt atagtcggcg agcagcagct caccgatcgc tttctgcgtg
     4321 ccatagctgg tcaggggcgt cgtgaagaat tcatcgccga tcttctccgg gaacggcgca
     4381 ccgaagacgg cgattgacga ggtgaagacg aggcgcggct tccgggcggc gccgtcatag
     4441 gcgcggcgga tgctgtcgag gagattcatg gtgccggcaa ggttgatccg gtagcccttg
     4501 tcgaagtcct gctcggcctc gcccgacacg atggccgcga gatggaagat gacatcgggc
     4561 ttaccggcga cgagcttgtc ggtctcgccg gccgccggga gatcgcctgt cgatatctcg
     4621 accgggaact ttgcaccagc gggcttctcg gccgccacca catcgtagag cgcgagctgc
     4681 gagatcgcct tgccgccgag tgtgccgtcg cgggcgagac gctcggcgag cttgcggccg
     4741 accatgccgg cgccgccgac gatgagaacc ttcatgatgt gcctcccttg ccgacgagcg
     4801 cgccggcagt cgattgatgc cttgatggcc aaggatgcgc gccatnnnnn nnnnncaggc
     4861 ccacgacgca agggcaatcg aggcgcaaga gcacggcggt ccgcgcctgc cgctcgcgcg
     4921 cttgtgaagg cgtttgaatg gacgtgggcc gcagaggccg acacactcaa gtccatgcgg
     4981 gcacgaggca tcatcagact ggtcacggtc gcggtcctgc tattggcagg tgcgctcgcc
     5041 gtcacgagcg ccgaggcgtg gggtcatctc ggtcacgaca gcggtatcga gagcgtctca
     5101 agcggcgatc tgctgcatgg ccctgatgca ccgcgcctcg agttgcggga ggcatcgaga
     5161 gtgccctgca agcgttgccc acgtccgcga tccgccgcga cctgtggtgt ggcgacgtgc
     5221 gtgacgccgg caatcgtcgc gccggagagc gtcgtcattg tggcgcccgc gggcgacttg
     5281 ctgcgcccga cgacattcac tgcgctcacg gatcatcgtg agccgcccga gccaccgcct
     5341 cccaaatcct gaacgagcac gcttggcctt ccagcgcgtc cgccgcatcg gctcgccgac
     5401 gaatggacgc ggcatgtcca attgcctcat tcaggatcaa ctctcatgtc gtctcacagc
     5461 attctgcagt cgcgccttgc gggcgcgcaa ctcgccttga ccctacgcat tggtctcggc
     5521 ctcgtcttcg tcatcggtgg atgggccaag ctcgagcggc tgctcacgcc gtcgcgggcg
     5581 aagggcatcg tcgacgagta cgtcggcccg ctcggctaca tcaatcagac gttcctcgac
     5641 tggctatttc agagcccgct cggggtggtc gcgacgccgt ggaattttct gacactgctc
     5701 tcggcgttcg aactggtctc gggaattgtg ctgatcgccg gcctgatggt gaggccgctt
     5761 gcggtgttct gggcgttgct gctctggagc ttcgtgttct ccctccctgt cgtcacgaca
     5821 ccgggcgtga agccgccggc cgcgacctac atgtcaccgg ccgagttcgt ccagatccgg
     5881 gacatcgcgc tctcggggtt cttcttcgtc ctctacaatc tcgggccggg cgcgcgcagc
     5941 gtcgatcacg acgttttcgc tgtcccggcg gagcgtccgc atgactggga agcgctcggg
     6001 ctgctcctcc gcctgtcgct cggcgtcgtg ttcctcgtcg ggggcctctt cgctggctac
     6061 aacaagatca cgactttcgg catgccgggc ctgcttctga cgttgatcgg cgcgggcctc
     6121 gtcgcgggcc tgtggacgcg tacgttcggc gcggctgcgg cggcgctgct cgtgtacttc
     6181 atggtctcga agcttgcggg tgccagtggc atcgtcggct atctgaactc ggtgaagcgc
     6241 gaattcgccc tgctcgccgg cgcaggtgtg cttgctcttg ctggtggcgg ctcgcggttc
     6301 gcggcgaccc gcgccattca gtcgttcaat gagtggacgc acatctacgt gggcggccag
     6361 cgcgtgagcg aggcaattcc cgcgcgacag aagtaacgac cggcgcccgg ggcggacctc
     6421 tccccgggcg ccgcgacttc gcctcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnncctggag cctcgtctgg tttctggttg gcggctgggt ctccaccgtt gcgcagatcc
     6601 tggtgatcgt cggggtgatc tttctctaca agtacggctg gcagcgcgcg ccgctcgaga
     6661 tctggtcgcg gagccgcaat ctcgcgcgtt tcgtgtgggc gtgggcgcgt cagcgcgagc
     6721 tgccggcggc agagaaccgg cgcggcgagg cagcgcggga tgtcgtccgt gtcgtaaagg
     6781 tgaaggaatt cggtgacgtc aacctgtcga cgctgctgtc gctggtcatg atcggcggca
     6841 tcgtgctgct gtcgacgggg ttctaacggg aacggatgat gacaggtcca ctcgacggca
     6901 aggtcgcaat cgtcacgggc tcggcccgca acatcggccg cgagacggca tcggcgctcg
     6961 ccgcaatggg ggcggccgtg atgctgcacg ccaagagcga tcgagacggg ctcgaggagg
     7021 cgaaggcctt gctcgccgcg cgcggggctc gcgtggcggg gcatctcgcc gacctctcga
     7081 cggaggccgg cgccaatggc ctcgtcgcgg cggcggttgc ggatctcggg cgggtggaca
     7141 tcctgatcaa caacgcggca atccggcgca atacctcgtt cacgacgatc acgctcgcgg
     7201 aatggcgtga ggtgctcgga gcgagccttg acgcagcctt cctgacgagc cgcgcggcag
     7261 tgccgcacat gcggggcggc aagtggggcc gcatcgtcaa cgtcggtggc atcaccatgt
     7321 tcaaggggac gcccgggcgt gcgcacgtcg ccgccgccaa ggccggcatg atgggcatga
     7381 cgcgcgtgct cgcgagcgag ctcgcgccgg acggcatcac cgtcaacnnn nnnnnnnatg
     7441 atctcgcccg gcatcatcga tacgaagcgc ggcgcggcgg ctggtgcgcg acccgccgcg
     7501 acgcagacgc tggccggtgt gccgcttggc cgcgagggga caacggccga gatcgcccat
     7561 gtcgtcgcga tgctgtgcca tccgaacggc ggctacacca cgggccaggt gctcgaagtg
     7621 aacggcggca tgcacttctc gtgaggtgca cctgtcggcg gtcgcattgg tcattgaatg
     7681 atctgcggga ccccagtaga gacgagacgg agcacgctca gttggcggat caggataggt
     7741 cgggttctct catggtacct cgtcgcgcgt ctcaagcagg tctggttcgc gccgccagca
     7801 ttgcggcgat tgccggcacg atgttgcttg gcgttgttga cggcgcgttc gccgagccga
     7861 ggccatcgcg caacagcggc acggccaagt ccgctggccc tgctgtgacc gtcgcgccac
     7921 aggacgacgt cggccggcct ggcgatgtcg tggccttcaa ggcgaagtat ccgatcggcg
     7981 ccatcgtcgt cgtgaacgac gatcgccgcc tcttctatgt gctgggcgac ggccgagcga
     8041 tccgctatcc gattgccgtc ggctccgagg acaatctgtg gacaggccag tccttcgtgc
     8101 agtccaaggc caaggatccc aagtggattc cgccctggga gccggaacgc gaagtggaag
     8161 ctggtcctga caatcctctt gggccacgcg ccatctatct cgggtggacg ctttaccgca
     8221 ttcacgggac taattctccg cgctctattg gtggcgctgc gtcgagtggc tgttttcgca
     8281 tgtacaacca ccacgccaag gatctctacg agcgcgtgca tatcggcgcg ccggtgtatg
     8341 tggtcgattc actcgacgat taggtgagtg gtactggggc tgccgggggg cgggacctca
     8401 gccgaacaag acaaccgaca atagttgccc gcgaacaacc accgcgcggc gcgattgtcg
     8461 taacaacgtc ggacggagtg aacgatttgt tcactaacga acgacaccat gtcggctcga
     8521 gagttaagtg gcggttccgg gctgtccgga tccggtacgg cgcagtcgac ctttgtgaac
     8581 tcgtcagttc gagtgcacgc ttggacaacg cgccgataat caggggacga ctatgatcgg
     8641 acgtgcaaga gttgggttta tcgcgctcgc ctttgcggcg gcggcaatga tttccatccc
     8701 gggcgaggcg aaagctcaga gcggcgattg gctctggggc ggctcgttcg tgtccggagg
     8761 cagcggccgc gaggacgtgc gtctcaacac cacctacggg cgcggcacga tcatcgtcag
     8821 cttcagcgat cgtcggctct acctcgtcac cggtcccggc accgcgatca gctatccgat
     8881 cgccatcccg cgcgaccaga gccgctggca gggtacaacc aacgtctcca tgaagaagat
     8941 caatccgccg tgggttccga ccgcgaccat gcgtgccgag aacccgcgcc tgccgaactt
     9001 cgtgcccggc ggccatccgc tgaacccgct cggcgtgcgt gcgctttatc tcggctcgag
     9061 cgcttaccgc atccacggca ccgacgcccc ctggaccatc ggccagaacg tgtcgaaggg
     9121 ctgcgtgcgc atgtacaatc aggacgtgat cgacctctac aatcgcgtgc cagtcggcac
     9181 ccgagtcatc gttacctggc agcgcttcca ggccggcgcg ccgatggtgt cgatggcacc
     9241 tatctccgac gacgaggcgc gcccgacgcg ccgctactcg cggtccaccg tgccgacgcg
     9301 tcgcgccgtg cgtacgaaga gcgcctcggc cgccgcggcg gagtgaggac gccgggttct
     9361 gcagtctgat ctgcgaaagg ggggcggctc gaccgcccct ttgtcgttgc tgcgcccggc
     9421 acgggcgcgg agcggacacc acggagcgga tactggcctt gcgaaggttg cgggccatgc
     9481 ttttatttgg ttggtagaag tgctagacca aagtcggtca gcattgctga ccttttgacg
     9541 tcgagccaac gatacagtgc ggatcagcgc tgcggcggtc gcgggatgct ccctcgagca
     9601 tcgcgaccgc aggacgcacg aaggtcctga agtcagacga cgcaaccgac gaggtgcgcc
     9661 atgccgaaaa gcagcccgag cctcaacgac aagttcgacc tcgacgagca ctggcagctt
     9721 ctcaacgggt cccaggcgat cgtccgtctg accttgatgc agcgcgcgcg cgatgtgaaa
     9781 gcaggcctca atacggcggg cttcgtgtcg ggttaccgcg gctcgccgat cgcagggctc
     9841 gaaggcgcct tccagcgcgc cgaggccatc acgcgcgaga atcacatccg cttccatccg
     9901 ggcctcaacg aagacctcgc ggcaaccgcg ctctggggcg cgcagcaggt cggcgtcata
     9961 aaggaggagg cgaagtacga cggcgtcttc ggtatctggt acggcaaggg tccaggcgtc
    10021 gaccgcacgg gcgacgtgtt ccgccatgcg aaccacgccg gcaccgcgaa gcacggcggc
    10081 gtgctggcgc tcaccggcga tgatcacacc tgcgagagct cgacctcggc gcaccagtcc
    10141 gagttcgcca tggtcgacta catgatcccg gtcttcaatc ctgcgggcgt gcaggagctg
    10201 ctcgactacg gcatctacgg cttcgcgctc tcccgctatg cgggcgtgtg ggtcgcactc
    10261 aagtgcgtaa aggacaattt cgagagcacc gcgaccgtcg acggccgcct cgaccgcgtg
    10321 caggtggtga tccccggcga ggacgtgctg cggctgccgt cggacggcct caacatcaag
    10381 cccgccatgg cggatacggc gctcgccaag gagcagcggc tccatgacta caagcgtcag
    10441 gcgatgctgg cgtttgctcg cgccaacaag ctcgacaagc ttgtcatcag cggcggggac
    10501 gcgccgaaga tcggcatcat caccaccg
//
LOCUS       DAORNH010000145        10525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4704_length_10525_cov_2.619866, whole genome shotgun sequence.
ACCESSION   DAORNH010000145 DAORNH010000000
VERSION     DAORNH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10525
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4704_length_10525_cov_2.619866"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..223)
                     /locus_tag="PK264_14570"
                     /pseudo
     CDS             complement(1..223)
                     /locus_tag="PK264_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
     assembly_gap    255..382
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(383..548)
                     /locus_tag="PK264_14575"
                     /pseudo
     CDS             complement(383..548)
                     /locus_tag="PK264_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
     gene            522..2078
                     /locus_tag="PK264_14580"
     CDS             522..2078
                     /locus_tag="PK264_14580"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HRD77132.1"
                     /translation="MGAPEGAQIKWQFLGYDLILAKVDKLSRLFALVFAIMAFAGGLF
                     ALNQRRTLERSAAFAYAGSAIGAVMAGDLISLFMFWELMAIGSTIVILCGAKGAEHAA
                     FRYAALHLLGGVLLMTGIAGEIQATGSIAFVKIDTSTLSRWLILAGFLINAGAPPLSA
                     WLPDAYPQASWSGMVFLSAFTTKSAVYVLIRGFPGTELLIWVGLFMVFYGIIYAILEN
                     DMRRILAYSIVNQVGFMVAGIGIGTEMALNGASAHAFTHIIYKALLLMSAGSVLLMTG
                     KSKCTDLGGLFRTMPLTCLCGIIGALAISSFPLTSGFISKSMIAEGAASRHLMWVWLA
                     LAAASAGVFLHAGIKFPWFVFFQKDSGLRPAEPPINMLAAMILLAALCIGLGVLPGPL
                     YALLPFKVEYVPYTAAHVLFQLQLLLFSGLAFFLLLPLLKRTLTITLDTDWFWRRAGR
                     WLLGGLDDVSSRAWDSIVRRALSGARTLAHGLHATHGPQGILGRPWPTGRMALWTMLM
                     LGAYLVVAYL"
     gene            2089..2430
                     /locus_tag="PK264_14585"
     CDS             2089..2430
                     /locus_tag="PK264_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77133.1"
                     /translation="MQSIQALRQTLDRWLYPDLSKVAPHHRERAMQRARSEAFDFLEI
                     CGLAAAIAAVTYFTRYALKAPSVSDRLSAAIANFIVAIPQLVLFAGPLLVRRNRRGLR
                     AFLDELNPPKT"
     gene            2482..2775
                     /locus_tag="PK264_14590"
     CDS             2482..2775
                     /locus_tag="PK264_14590"
                     /inference="COORDINATES: protein motif:HMM:NF022833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRD77134.1"
                     /translation="MLGLFSRRYAPFIYGVIQSAVTTGVATAIATYQLTGVGMEFVKR
                     WLLVWAIAWLTMLPIVLLVAPVIQRAVFALTGQPAQSSASPRKRHRRPHRRNR"
     gene            complement(2974..4191)
                     /locus_tag="PK264_14595"
     CDS             complement(2974..4191)
                     /locus_tag="PK264_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRD77135.1"
                     /translation="MTIGVGGSTAEQELARLTPMRGSVPPIGVDERLARVARAQALMR
                     EKGIDALWLDTSTSLTYFTGLKMRRTERLHGAVLPAKGEIVYLSPTFEVEKLKTMLSF
                     GDRIAAWQEDEDPTALVTETIRSLGIARGTIAIDEATPFFTFDGLRRSGNAYTFVNAI
                     DITGACRAVKSEHEVALMQTAKNITLEVHKAAARIMREGITTTEVQAFNVAAHRKLGA
                     EGDPPFNIVLFGEATAYPHGVPHPQTLREGDMVLIDVGAPIDGYLSDVTRSYVFGEPS
                     SRQRQVWNLEKAAQAAGFAAAKPGNRCEDIDIAARGVITAGGFGPGYATPGLPHRTGH
                     GIGLDVHEWPYLVKGDRTTLRPGMTFSNEPTICIYGEFGVRLEDHMLITETGARWFTE
                     PAHSVDDPFGYEV"
     gene            complement(4188..>4668)
                     /locus_tag="PK264_14600"
     CDS             complement(4188..>4668)
                     /locus_tag="PK264_14600"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRD77136.1"
                     /translation="ADVIKRVRPLMERLGRLTHMGPVGAGQATKLVNQTLVLTNYCVM
                     AEAFRLAEAYSVDAAKVPEALASGHAGSNLLPILFERMNARDYAPRGYARQVLKDLEM
                     LNDAAHEKGLAMPMSAQALTLYRLLVANGRSELDGAAIATLWPERDGRGPGTKGDKS"
     assembly_gap    4669..4732
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4733..5011)
                     /locus_tag="PK264_14605"
     CDS             complement(<4733..5011)
                     /locus_tag="PK264_14605"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD77137.1"
                     /translation="MVGCDLVPERREAARAAGIVIAATAAEVAAEANAICLCLTNTGA
                     VAQVVEGASGIVSAGHRDSLVVIDHSTTEIEATKRLAAVLRERAGAAFI"
     assembly_gap    5017..5112
                     /estimated_length=96
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5204..6370
                     /locus_tag="PK264_14610"
     CDS             5204..6370
                     /locus_tag="PK264_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HRD77138.1"
                     /translation="MLKDGQNIHVTRDELLSRQRATIAEMERRGLSALLVFRQESMYW
                     LTGYDTFGYVHFQCLVLTADGRTVILTRSADKLQARFTSIVPDIRIWVDRQGANPALE
                     LRAILAEHGLSGRRLGIEMEAYGLTARKWKHVEAALDGFSLLDDASDLVTALRCVKSP
                     AELAHIRRAAALADEALAAAEATTRPGAFEGDILAAMQGTIYRGGGDDPANEQIIGSG
                     PGALMCRYYSGRRHLDADDLLTLEFAGVYRHYHACLMRTFVTGRLDPRIAEMHKAAVE
                     ALHASEQALVPGRPISEVFDTHARTLDHLGYAAQRLNACGYSLGATFAPNWMDWPMFY
                     AGNDYEARPGNVFFVHMILFDEREGLAMTLARTSIVTERGAEPISKASLELVRR"
     gene            complement(6524..6934)
                     /locus_tag="PK264_14615"
     CDS             complement(6524..6934)
                     /locus_tag="PK264_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77139.1"
                     /translation="MIGNGDIDRFVIGVCEIGIILEVLVAVARGAAVDAAGMLLLVAV
                     GLGAAAVDIHALEEDDVLGMRLEGRDRRASKHGDGGWVGAARIEGAALEEMDVARGLG
                     ERRRRGISHAAQHRLEEQGRKSQCLDEISPRHGF"
     gene            6915..7934
                     /locus_tag="PK264_14620"
     CDS             6915..7934
                     /locus_tag="PK264_14620"
                     /inference="COORDINATES: protein motif:HMM:NF019222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HRD77140.1"
                     /translation="MSPLPITMPAAPIRSLHIGGGAHSDADHVGVGNRFISFRKRQPG
                     DARYGNAIEPIQDAGQVFDLLIKAAGNACSAHARQPGRATEDSAALASRRASVVDFIL
                     SDVKDAKRRFGMDTEHARLLDGMLENWSQTEKAARAASAPTAPKTAPVTTAACALPTR
                     PTARGNNKHSLDDLSPVHDQMIAMVKLAFELDLTRVVAFTLSGGSSGQTWPSRGVHRA
                     HHSLEHSGDVESLVKIDTYYAEKFAKLLAALKSIDDGGGRSALYNSSVFLGMECWSNS
                     SSGHYLTNIPFVFAGQGGGRFETGRIVNAGGRNNNDIHISCLNAAGLATSTFGLANLC
                     RGPII"
     gene            7946..9583
                     /locus_tag="PK264_14625"
     CDS             7946..9583
                     /locus_tag="PK264_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HRD77141.1"
                     /translation="MTGALSRLFDIRIAGLLASVAVGAALVATPAPQAETAPTKSVAK
                     AAPGSEAFLSEAAEKYFPAEDAAPSPKRVFRLTRDQIDATVRALLPGYVTASIKETMP
                     RDPLQTNYEYAEILNFNASNLGPLTGWIGGIAARVRASPKGVIDCAASGNAPDCLSVS
                     ARRFVVQAFRADAPDEKIESIVAFFVKGVSANGPAQATGDLVEVTLNSPYFLFRRELE
                     TSRRGRLGAVQHLQAITFTLADTPPEALGLSSGDALQYLQTASDARASVDNVLASKPA
                     REKLLRFFRSWLEVREPGDFLISRETFKEFTPEFAAAMVAGTDALLREALATPAPSLK
                     DITTLTRRDTGTDPSRRFGIFTDPAVLASHSGPTNTRFVKRGVFWVRKVMCMEMATPS
                     KEVLQAIEQSYAPLVTTERARIEGMTKPPECAGCHKLIDPFGAILESYDALGRWRTSD
                     NGHPIDQTVAIDFLDEGPVTVRSPVEALKALTGSVMFKQCFVRQAFRYYMGRNEEPSD
                     EPLLRAMFLAFAKDDKQDILGLIRSLALSETVSRRQM"
     gene            complement(9602..>10525)
                     /locus_tag="PK264_14630"
     CDS             complement(9602..>10525)
                     /locus_tag="PK264_14630"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD77142.1"
                     /translation="DSQAFLDLIKKLGRPPINSLPPLQAREVFRRGRLVTQPDLPQVD
                     RVKEYTFDGPGGPVPVRVYRGAGTADLGALPVHVYYHGGGWVLGDLDSHDWCCRKIAN
                     GALCAVVSVDYRMAPDAVFPAAFEDSLAAVKWTKANAAMLKVDPARISVGGDSAGGNL
                     AAAVAIALRDEGDIKLKAQILVYPAVDLSMSGDYYGRHTKDLVLTDEVMRWFIDLYTP
                     DAAMRKDWRASPMLAPSHKGLPPALILLAGFDPLYPEGERYAAMLEGAGVPVTKKVYP
                     GQIHAFFSNGKLLPKANQAIEDVVAALKAHH"
BASE COUNT         1630 a         3567 c         3202 g         1838 t
ORIGIN      
        1 cctgttcctc ggccgacagc gtctcgtcga cgacgaggcc atggccctcg ccctcgacca
       61 cggtcagcca catctccggg tgcttttcgg tcgcctgcgc gcgcctgagg cgcccggcgc
      121 cgatgcggcc atgcgcgccg gggccgacgc cgacatagtc gccatagcgc cagtagacga
      181 gattgtgccg gcattcctcg ccgcgccgcg cgtggttcga caccgcgcgt caagccctgg
      241 tcacggctgt gatcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nncggcgcgg ccgagcgcgg tcttcagctc ctcgcgccag
      421 cgctgcggcg tctggccggg gcgggcgtag atcaggtcga aggaaaagcg cgagaaggcc
      481 gagctcgcca cgtcgagcgc cgccagcgcc tcggcgacgg aatgggtgcg cccgagggcg
      541 cgcagatcaa gtggcagttc ctgggctacg acctcatact cgcgaaggtc gacaaactct
      601 cgcgcctgtt tgctttggtg ttcgcgatca tggcgttcgc gggtggtctg tttgcgctca
      661 atcagcggcg gacgctcgag cgttctgccg cgtttgccta tgccggcagc gccatcggcg
      721 cggtcatggc cggcgacctg atctcacttt tcatgttctg ggagctgatg gcgatcggct
      781 cgaccatcgt catactgtgc ggggctaagg gtgccgagca tgcggcgttc cgctacgcag
      841 cattgcatct cctcggtggc gttctgctga tgacgggcat cgcaggcgag atccaggcga
      901 ccggctccat cgcattcgtc aagattgaca cgagcacgct gtcgcgctgg ctgatcctcg
      961 ccggcttcct gatcaatgcc ggcgcaccgc cgctgtcggc atggctgccc gatgcctacc
     1021 cgcaggcgtc ctggtccggc atggtgttcc tctcggcatt caccaccaaa tctgccgtct
     1081 atgtgctcat tcgcggcttc ccaggcaccg agctgctgat ctgggtcggc ctcttcatgg
     1141 tgttctatgg gatcatatac gcgatccttg agaatgacat gcggcgcatc ctcgcctact
     1201 cgatcgtcaa tcaggtgggc ttcatggtgg ccggcattgg tatcggcacc gagatggcgc
     1261 tcaacggcgc ctcggcgcac gccttcacgc acatcatcta caaggcactg ctgttgatgt
     1321 cggcaggctc ggtgctgttg atgaccggca aatcgaagtg caccgatctc ggtgggctct
     1381 tccgcaccat gccgctgacg tgcctgtgcg gcattatcgg cgcgctcgcc atctcatctt
     1441 ttcccctgac ctccggcttc atctcgaaat cgatgatcgc cgaaggcgct gcgagccggc
     1501 acctgatgtg ggtctggctt gcactcgcgg cggcctccgc cggcgtcttc ctgcatgccg
     1561 gcatcaagtt cccctggttc gtgttcttcc agaaggattc tggcctcagg cccgccgagc
     1621 cgccgatcaa catgcttgcc gctatgatcc tgctggccgc gctctgcatc ggcctcggcg
     1681 tgctgccggg accgctctat gccctgttgc cgttcaaggt cgagtacgta ccctacacgg
     1741 cggcgcacgt gctgtttcag ttgcagctgc tgctgttctc cggcctcgcc ttcttcctgc
     1801 tgctgccgct gctgaagcgc acgctcacca tcacgctcga caccgactgg ttctggcgcc
     1861 gcgccggccg ctggctcctg ggcggcctcg acgatgtgag cagtcgcgcc tgggacagca
     1921 ttgttcgacg cgcgctcagc ggggctcgca cgctcgcgca cgggcttcac gccacgcatg
     1981 gcccacaggg cattctcggc aggccctggc cgaccggccg catggcgttg tggaccatgc
     2041 tgatgcttgg cgcctatctc gtcgttgcat atctgtagga ggcgcgctgt gcagagcatc
     2101 caggcgcttc gccagacgct cgatcgatgg ctctatcccg acctcagcaa ggtcgcgccg
     2161 catcaccgcg agcgtgccat gcagcgggct cgctccgagg cgttcgattt cctggaaatc
     2221 tgcggtctgg ccgccgcaat cgcagccgtg acctacttca cgcgatatgc actcaaggcc
     2281 ccctcggttt ccgatcggct gtcagcagcg atcgccaact tcatcgtcgc catccctcaa
     2341 ctggtactgt tcgccggccc gctgctcgtg cgccgaaatc ggcggggttt gcgcgcgttc
     2401 ttggatgagc taaatcctcc aaaaacgtag ccgatctcgc ggccatttca gatcgatgcc
     2461 gactggcgga atatggaagt catgctgggt ctattttcga gacgatacgc accgttcatc
     2521 tacggtgtca ttcaatcggc cgttacgacg ggcgtcgcga cagccatcgc cacgtaccag
     2581 ctcacgggcg tcggcatgga attcgtgaag cgatggctct tagtctgggc gatcgcctgg
     2641 ctcacgatgc ttcctatcgt gttgctcgtc gccccagtca tccagcgggc agtctttgcc
     2701 ctgactgggc agcccgctca atcctctgcc agcccccgaa aacggcaccg tcgcccacac
     2761 cgccgaaatc ggtaacgctg gatcttgtgc atcccgatgc gtggcgttcg aaggcgaagc
     2821 tacaacggca gctgatcccc cagatgcgcc cgtccgaagg cgtatccatt ggcaatggcg
     2881 atgcaccctt ctggaccatt gcgttcgcca tgttcttccg gtgtcgagga agcctcttcc
     2941 ccgcttatcg atgccgcccc tgccccgtcc gtttcacacc tcatagccaa aaggatcgtc
     3001 gacggaatgc gcgggttcgg tgaaccagcg ggcgccggtc tcggtgatca gcatgtggtc
     3061 ctcgaggcgg acgccgaact cgccgtagat gcagatcgtc ggctcgttgg agaaggtcat
     3121 gccgggtctc agggtagtcc ggtcgccctt cacgagatag ggccactcgt gcacgtcaag
     3181 cccgatgccg tggcctgtcc gatgcggcag gcctggcgtt gcatagcccg gcccgaagcc
     3241 gcccgccgta atcacgcccc tcgccgcaat gtcgatgtcc tcgcagcggt ttcctggttt
     3301 cgcggccgca aaacccgcgg cctgcgcggc cttctcgagg ttccacacct ggcgctggcg
     3361 gctcgatggc tcgccgaaca catatgagcg cgtgacgtcc gagaggtagc cgtcgatcgg
     3421 cgcgccgacg tcgatcagca ccatgtcgcc ctcgcgcaag gtctgcggat gcggcacgcc
     3481 gtgcgggtag gccgtcgcct cgccgaagag cacgatgttg aacggcggat cgccctccgc
     3541 gccgagcttc cggtgcgccg ccacgttgaa ggcctgtacc tccgtcgtcg tgatgccctc
     3601 gcgcatgatc cgcgccgccg ccttgtgcac ctcgagcgtg atgttctttg cggtctgcat
     3661 aagcgcgacc tcgtgctccg acttcaccgc gcggcaggcg cccgtgatgt cgatcgcgtt
     3721 gacgaaggtg taggcattgc cggagcggcg gagtccgtcg aaggtgaaaa acggcgtcgc
     3781 ctcgtcgatg gcgatcgtgc cgcgggcgat gccgagcgag cggatcgtct cggtgacgag
     3841 cgccgtcggg tcctcgtcct cctgccaggc ggcgatgcga tcgccgaaac tcaacatggt
     3901 tttgagtttt tcgacctcaa acgtcggcga gagatagacg atttcgccct tggcgggcag
     3961 caccgcgccg tgcagccgtt cggtgcggcg catcttcaac ccggtgaaat aggtgaggct
     4021 cgtcgaggta tcgagccaga gcgcgtcgat gcccttctca cgcatcagag cctgcgcccg
     4081 ggcaacgcgc gcgaggcgct cgtccacacc gatcgggggc acgctgccgc gcatgggtgt
     4141 gaggcgcgcg agttcctgct cggccgtcga gccgccgaca ccaatggtca cgacttgtct
     4201 cccttggtcc ccggtccacg accatcgcgc tccggccaaa gcgtggcaat ggcggccccg
     4261 tcgagctcgc tgcggccgtt cgccaccagc agccgataga gcgtcagcgc ctgggccgac
     4321 atcggcatcg cgaggccctt ctcgtgcgcg gcatcgttga gcatctcgag atccttcagc
     4381 acctgtcgcg catacccgcg cggggcgtag tcgcgggcgt tcatgcgctc gaacaggatc
     4441 ggcagcaaat tcgaaccggc atgaccgctc gccagcgcct cgggtacttt cgccgcatcg
     4501 acgctatagg cctcggcgag gcggaaggct tcggccatga cgcagtaatt ggtcagcacg
     4561 agcgtctggt tgacgagctt cgtcgcctgt cccgcgccga cggggcccat gtgcgtgagg
     4621 cggccgaggc gctccatcag cgggcggacg cgcttgatca cgtcggctnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngatgaatg
     4741 ctgcccccgc gcgctcccgc agaaccgcgg cgaggcgctt ggtcgcctcg atctcggtcg
     4801 tcgagtgatc gatcacgacg aggctgtcgc gatggccggc cgagactatc ccgctcgcac
     4861 cctcgacgac ttgcgccacg gcgcctgtgt tggtgaggca caggcagatc gcattcgcct
     4921 cggccgcgac ctccgcagct gtcgcggcga tgacgattcc ggcagcgcgc gcagcctccc
     4981 tccgctccgg cacaagatcg catccgacca cctgatnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nncgttccgc ctcgccgctt cgccgcccgc caccgtcctg ttgcatgcta
     5161 ggatgccatg caaaacgcgg cgttccaaca caacggtacg gtgatgctca aggacggcca
     5221 gaacattcac gtcacccgcg acgagcttct ttcccgccag cgcgcgacga tcgctgaaat
     5281 ggagcggcgc ggcctctccg cgctcctcgt gttccgccag gagagcatgt actggctcac
     5341 cggctacgac accttcggct acgtgcactt ccagtgcctt gtgctgaccg ccgatggccg
     5401 caccgtcatt ctcacgcgct cggccgacaa gctgcaggcg cgctttactt cgatcgttcc
     5461 ggacatccgc atctgggtgg atcgtcaggg cgccaatccg gcgcttgagc tgcgtgcgat
     5521 cctcgccgag catggcctct ccggccgccg gctcggcatc gagatggagg cctatggcct
     5581 caccgcgcgg aagtggaagc atgtcgaggc ggcgctcgac ggcttctcgc tcctcgacga
     5641 cgcctccgat ctcgtgaccg cgcttcgctg cgtgaagagc cctgccgagc ttgcccatat
     5701 ccgccgcgcg gccgccctcg ccgatgaggc gctcgccgcg gcggaggcaa cgacgcgccc
     5761 aggcgccttc gagggcgaca tactcgcggc gatgcaaggc accatctacc gcggcggcgg
     5821 cgatgatccc gcgaacgagc agatcatcgg ctcgggtccc ggcgcactca tgtgccggta
     5881 ttactccgga cgccgccatc tcgacgccga tgatctgctg acgctcgagt tcgccggtgt
     5941 ctaccggcac taccacgcct gcctgatgcg cacgttcgtc accggccgcc tcgaccctcg
     6001 catcgccgag atgcacaaag ccgccgtcga ggcgcttcac gcgagcgagc aggccctcgt
     6061 ccccggccgg ccgatctcgg aggtgttcga cacccacgcc cggacactcg accacctcgg
     6121 gtacgcggcc cagcgcctca atgcctgcgg ctacagcctc ggcgccacct tcgcgccgaa
     6181 ctggatggac tggccgatgt tctatgccgg caacgactac gaggcgcggc cgggaaacgt
     6241 cttcttcgtc cacatgatcc tcttcgacga gcgcgaaggt ctcgccatga cactcgcccg
     6301 cacgtcgatc gtcacggagc gcggcgccga gccgatctcg aaggcgtctc tggagcttgt
     6361 gaggcgctga gcaagcggcg tctgacggtt cgccgcaatt cgtccctgat tcgggcgggg
     6421 actgccacat gcgcgccctg ctgccgcccg accggctggt gaagcatccc gacggggagc
     6481 ttcgagcagt gttggctgcg agggaaatgt catgcgtcgt ccgtcagaac ccatgtcgcg
     6541 gcgagatctc atcaaggcac tgggactttc ggccctgttc ctccagccgg tgctgcgcag
     6601 catggcttat gccgcggcga cgccgttcgc caaggccccg cgctacatcc atttcttcaa
     6661 gggcggcgcc ttctatccga gccgcaccaa cccatccgcc atcaccgtgc ttgctggcac
     6721 gccgatcgcg cccctcgagg cgcatgccaa ggacatcgtc ctcttcaagg gcatgaatat
     6781 ccacggcggc agccccaaga ccgacggcta caaggaggag catgccggcg gcgtctacgg
     6841 ctgcgccacg ggcaacagct acaagtactt caagaatgat gcctatttcg catacaccga
     6901 taacgaatcg atcgatgtcg ccattgccga tcactatgcc ggccgcgccg atcaggagcc
     6961 tgcacatcgg cggcggcgcg cactcggacg ccgatcacgt cggtgtcggc aaccgtttca
     7021 tctcgttccg gaagcgccag ccgggggacg cgcgctacgg caatgccatc gagccgattc
     7081 aggatgccgg ccaggtgttc gacctgctta tcaaagcggc cggcaacgcc tgctcggccc
     7141 atgcccgcca gccgggccgc gccaccgagg acagcgccgc cctcgcctcg cgccgcgcga
     7201 gcgtcgtcga tttcatcctc tccgacgtga aggacgccaa gcgccgcttc ggcatggaca
     7261 ccgagcacgc gcgcctcctc gatggcatgc tcgaaaactg gagccaaacc gagaaggccg
     7321 cccgtgccgc ctccgccccg accgccccga aaacggctcc cgtcacgacc gccgcctgcg
     7381 ccttgccgac acgcccgacc gcgcgcggca acaacaagca cagcctcgat gacctctctc
     7441 ccgtgcacga ccagatgatc gcgatggtga agctcgcctt cgagcttgat ctcacccgcg
     7501 tcgtcgcgtt cacgctttcg gggggatcca gcgggcagac ctggccgagc cgcggtgtcc
     7561 accgcgccca ccattcgctc gagcacagcg gcgatgtcga gagcctcgtt aagatcgaca
     7621 cctactatgc cgaaaagttc gcgaagctcc ttgcggcgct gaaatccatc gacgacggtg
     7681 gcggcagatc cgcgctctac aacagctccg tcttcctcgg catggagtgc tggtcgaaca
     7741 gctcgagcgg ccactacctc accaacatcc cgttcgtttt cgctggccag ggcggcgggc
     7801 gcttcgagac gggccgcatt gtcaacgcag gcgggcgcaa caacaacgac atccatattt
     7861 cctgcctcaa tgctgcgggc ctggcgacga gcactttcgg tctcgcgaac ctctgccgcg
     7921 gtccgatcat ctgaggagcg gcagcatgac aggagcactc agccgcctgt tcgacatccg
     7981 catcgccggt ctgctcgcct cggtcgcggt cggcgcggcg ctcgtcgcga cgcctgcgcc
     8041 gcaggcagag acggcgccga cgaagtcggt cgccaaagcg gctcccggct ccgaggcctt
     8101 cctcagcgaa gcggccgaga agtactttcc ggccgaggac gccgcgcctt cgccgaagcg
     8161 cgtcttccgc ctgacgcgcg accagatcga cgccaccgtc cgcgcgctcc tgccgggcta
     8221 tgtcacggct tcgatcaaag agacgatgcc gcgcgacccg ctgcagacga actatgagta
     8281 tgccgagatt ctcaacttca acgcgtcgaa cctcgggccg ctgacgggct ggatcggcgg
     8341 gatcgctgca cgcgtgcgcg caagtcccaa gggcgtgatc gactgtgcag cgtcgggtaa
     8401 cgcgcccgac tgcctctcgg tcagcgcccg acgcttcgtc gtccaggcct ttcgcgcgga
     8461 tgcgccggac gagaagatcg agagcatcgt cgccttcttc gtgaagggcg tctcggcgaa
     8521 cggccccgcg caggcgaccg gcgacctcgt cgaggtgacg ctcaactcgc cctacttcct
     8581 gttccgccgc gagctagaga cgagccggcg cgggcggctc ggcgccgtcc agcatctcca
     8641 ggccattacc ttcacgctcg ccgacacgcc gcccgaggcg cttggcctca gctcgggcga
     8701 cgcgctccag tatctgcaga ccgcgagcga tgcgagagca agcgtcgaca acgtactcgc
     8761 ctccaagccc gcccgcgaga agctccttcg ctttttccgc tcctggctcg aagtccgcga
     8821 gccgggtgac ttcctgatct ctcgcgaaac cttcaaggag ttcacgcccg agtttgccgc
     8881 cgccatggtc gcggggacgg acgcgctcct ccgcgaggca cttgccacgc ccgcgccgag
     8941 cctcaaggac atcaccacgc tgacgcgccg cgacacgggc actgatccgt cccgccgctt
     9001 cggcattttc accgatccgg ccgtgcttgc gtcacactcc ggaccgacga acacgcgctt
     9061 cgtaaagcgc ggcgtcttct gggtgcgcaa ggtgatgtgc atggagatgg cgactccgtc
     9121 gaaggaagtg ctccaggcga tcgagcagag ttacgcgccc ctcgtcacga cggagcgcgc
     9181 gcgtatcgag ggcatgacca agccgcccga gtgcgccggc tgccacaagc tcatcgatcc
     9241 gttcggcgcc atcctcgaga gctacgatgc cttgggtcgc tggcggacgt cggacaacgg
     9301 ccacccgatc gaccagacgg tcgcgatcga ctttctcgac gaggggccgg tcacggttcg
     9361 ctcgccggtc gaggcgctga aggcgctcac cggatcggtc atgttcaagc agtgcttcgt
     9421 gcgccaggcc ttccgctact acatgggccg caacgaggag ccgtcggacg aaccgttgct
     9481 ccgcgccatg ttcctcgcct tcgccaagga cgacaagcag gacatcctcg ggctgatccg
     9541 ctcgctggca ctgtccgaga ctgtcagccg ccggcagatg tgaggccggc cgcaccgccc
     9601 ctcaatggtg tgcctttaag gccgctacca cgtcctcgat cgcctggttg gccttgggca
     9661 gcagcttgcc gttcgagaag aaggcgtgga tctggccggg gtagaccttc ttcgtcacgg
     9721 gcacgcccgc gccttcgagc atggcggcat agcgctcgcc ctcgggatag agcgggtcga
     9781 agcccgcgag caggatgagc gcgggcggca ggcccttgtg gctcggcgcc agcatcggcg
     9841 aggcgcgcca gtccttccgc atcgccgcgt ccggcgtata gaggtcgatg aaccagcgca
     9901 tcacctcgtc ggtcagcacg aggtccttcg tatgccggcc gtagtagtcg ccactcatcg
     9961 aaaggtcgac ggccggatag acgaggatct gcgccttgag cttgatgtca ccctcgtcgc
    10021 ggagcgcgat cgccaccgcc gcggcgaggt tgccgccagc gctgtcgcca ccgacggaga
    10081 tgcgcgcggg atcgaccttc agcatcgccg cgttggcctt cgtccatttc acggcggcga
    10141 gcgaatcctc aaatgccgcc gggaacactg catccggcgc catgcggtag tcgaccgaga
    10201 cgacggcgca gagtgccccg ttggcgatct ttcggcagca ccagtcgtgg ctgtcgagat
    10261 cgccgagcac ccagccaccg ccgtgatagt agacgtggac gggaagcgcg ccgagatcgg
    10321 ccgtgccggc gccgcgatag acgcgcacgg gcaccggtcc gccggggccg tcgaacgtgt
    10381 attccttcac gcggtcgact tgcggcaggt ccggttgcgt cacgagccga ccgcggcgga
    10441 agacctcgcg cgcctgcaag gggggcagcg agttgatcgg cggtcggccg agcttcttga
    10501 tcaggtcgag aaaggcctgg ctgtc
//
LOCUS       DAORNH010000146        10518 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4709_length_10518_cov_2.969225, whole genome shotgun sequence.
ACCESSION   DAORNH010000146 DAORNH010000000
VERSION     DAORNH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4709_length_10518_cov_2.969225"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..2065
                     /gene="glnD"
                     /locus_tag="PK264_14635"
     CDS             35..2065
                     /gene="glnD"
                     /locus_tag="PK264_14635"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HRD77143.1"
                     /translation="MADMQPIKPPVFTKQEQATYDRCERFLWAVRCHLHFLTGRAEER
                     LSFDVQPELASRLGYATRRGSMPVERFMKHYFLIAKDVGDLTAVLCSALEVAQLKTSP
                     GLGSLLNPLTWRMRRRVRATSDFRVDNGRLNVADEKVFERDPANLLRIFAHAEHAQTF
                     LHPDAIRLIRRSVPTLGPRLRMSDDANRIFLELLTSKRGSPEAALRRMNEAGVLGRFM
                     PEFGKVVSMMQFNMYHHFTVDEHLVRTVGQLYEIEQGALAKELPLSTRIFSSIQGRRA
                     LFVAGFLHDIGKGRPEDHSILGARIARTICPRLGLTDAETDTIVWLIEQHLFMSNTAL
                     SRDLADPKTIRDFADVVQTPERLKLLLLLTVADIRAVGPGTWNGWKGQLLRTLYAETE
                     LQLTGGHTMSSHKARVGAAQDELRTALAAWPKDDVEAFIDRHYPDYWLKSDVRRQMDH
                     AELMRRAAKTGKSLATECRTDEFTAITELTVLAPNHPRLLSLFAGACAHAGANIVGAN
                     ISTTRDGLALDTFQLQRENMEADERRRADRIADTIGRLLEGKARIGQLMAKRRPVEKR
                     VAAFAVTPEVMIDNALSDRLTVIQVSGRDRTGLLYELTSKLSDLNLDIASAHIATFGE
                     KAVDVFYVTDLTGKKIMGEARQRLIIDGLASVLASHGGLDALPEPAAAPVQA"
     gene            2216..3031
                     /locus_tag="PK264_14640"
     CDS             2216..3031
                     /locus_tag="PK264_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HRD77144.1"
                     /translation="MSDFFISVDELSAQIATSIAPIIIDCRRRPKFDEDPRTLPTARW
                     RDHTEVSNWAAQVPHGASVVLACIHGHNVSQLAASLLRERGIDARALAGGIEAWKARG
                     LPTLLKSALPGRSEEQPSRWVTRIRPKIDRIACPWLIRRFIDADAEFLFVEPDFVADV
                     AKEVGGIPYDIDGVEFSHEGPLCTFDTMIKRFGLSDPALDALALIVRGADTARLDLAP
                     EAAGLLAVSLGNSSLAGGDDRRALTLGFPVYDALYAWARHAKGETHNWPAKSA"
     gene            3068..4378
                     /gene="chrA"
                     /locus_tag="PK264_14645"
     CDS             3068..4378
                     /gene="chrA"
                     /locus_tag="PK264_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HRD77145.1"
                     /translation="MSTTDSGATPTYAEAFRVWLKIGILSFGGPAGQIAMMHRELVQE
                     RRWIDEQRYLNALNFCMLLPGPEATQLATYVGWRLHGVIGGITAGLLFVLPGAILMLL
                     LSLLYVVYGKVPIGEALFVGIKAAVLAIVVEALLRIARRGLKGSADKWIAGLAFVAIF
                     FLAIPFPAIVAAAAIFGFWRARGAPASAAAAPVSVPLSRTFRTALLWLAIWIVPLVAV
                     AMIFGKTHVLSQLAWYFSYLACFTFGGAYAVLAWMADDAVRYYGWLSPGQMIDGLGLA
                     ETTPGPLILVTQFVGFLAGYQAAGHGPALVLAVAGALVTLWATFAPCYLWIFVGAPYV
                     ERLSAEPRLKSALAAVTAAVVGVILYLTVWFALHVLFKIVREPSIAAGPLKLRAFVPD
                     GATADVRAVILAAAAFVLVFALHRSLIATLAACGGAALAWHWIG"
     gene            complement(4398..5291)
                     /locus_tag="PK264_14650"
     CDS             complement(4398..5291)
                     /locus_tag="PK264_14650"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD77146.1"
                     /translation="MAIGFVGIGNMGLPMAEKLIDGGHALIIHDLHAERLAPLTARQA
                     RPAASPREVGDQCEIAIASLPTLPSLEAVCLGESGLIHGKAVRIVVNTCTVGIALVEK
                     IATGLAAKGITLVDCPISGGPPGARDGILSVMLSGDPAAVAKVRPIVSLWGKQVTVAG
                     DKPGAAQLLKLTNNILSGVALVASAEAFVMGAKGGLDPEVMLAAINAGSGRNSATLHK
                     FPISLMDRSFAFGATMDTILKDVELAIAQGEALGVPMWVSQAARLIFKHAVFKGAGQD
                     DLTTIVRHVEAAAGFEIPKTR"
     gene            5454..6329
                     /locus_tag="PK264_14655"
     CDS             5454..6329
                     /locus_tag="PK264_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77147.1"
                     /translation="MIRTRKIGDATVTSVIEYSGPTHAPEFLFEKDLDRARLKANERW
                     LAPNHYVPKMDRFIITIQLWILKAGGNTIIVDTGVGNLKPRAAERMNMLNTLVLPWLE
                     AAGAGPNEVTHVVHTHMHTDHVGWDTTLKAGTWTPTFPKARYLYPKRDFDDLASAAKA
                     GKPDPAFADSVMPVVEAGLADMIDPEKEAAGCLAVEPAPGHSHGMVTFRLRSRAEEAI
                     FSADVMHHPIQVAEPSWNSRYCVAPDQARKTRAELLARAADRNALIMPMHFGAPYCGY
                     IRRQGSGYRFEGAGW"
     gene            complement(6357..7451)
                     /locus_tag="PK264_14660"
     CDS             complement(6357..7451)
                     /locus_tag="PK264_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRD77148.1"
                     /translation="MATYPGAETEKRIPLALLATTVQDVFRAAGLAAGAAGTVAESLT
                     SADLRGIHSHGVLRVPDYVKKLLDEGVDPRGAPRLISDKGAALVVDGGNNLGQVAMRF
                     AMEQAIARAKEINVAFAAVRGSNHAGTMDYYVRMALAHDMIGIAGTNALPTMAPWGGR
                     DKIVGINPIGIAIPGSDGAAFVLDCAFGATAHGKIRVYAQKGEPIPAGWAYDAHGQPT
                     TDAVAALDGLIQPIGAFKGVGLGMAIGMLSSLLSGAAYGTESGNMVDGPVAGVDGQFA
                     LAINIAAFTDVDRFKSRVDRILEQIRKSSRAAGVDRLYSPGEIEADFEAAYRRDGIPL
                     NDETLRGVIASAGRVGANVAAFTAHVGPLA"
     gene            complement(7547..>8047)
                     /gene="gstA"
                     /locus_tag="PK264_14665"
     CDS             complement(7547..>8047)
                     /gene="gstA"
                     /locus_tag="PK264_14665"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HRD77149.1"
                     /translation="EGGGDYKAVNPLGYVPALELDDGTILTEGPAIVQYLADKAPEKR
                     LAPANGTIERYKLQSWLNFLSTEVHKGFSPLFTPGMPEEGKTNARERLATRFAHLDAH
                     LASRDYIMGAGFTVADAYLFTTLRWTVPTKIDLAPYPNLTAFMKRVQARPAVQEAMKA
                     EKLPMH"
     assembly_gap    8048..8080
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8081..8160)
                     /locus_tag="PK264_14670"
     CDS             complement(<8081..8160)
                     /locus_tag="PK264_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HRD77150.1"
                     /translation="MKLYYAPGVCSLSPHIVAREAGLAVTL"
     gene            8341..10461
                     /locus_tag="PK264_14675"
     CDS             8341..10461
                     /locus_tag="PK264_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRD77151.1"
                     /translation="MSDTEKPLVRYEARDGIGIITIDNPPVNALGPGVSDGIISAVEK
                     AEADPAVKAMVMIGAGRSFIAGADIRQFGKNRPTPKKRTYDVLDETKKPIVAAIHGYA
                     LGGGLENALACHYRVAVPSAKVGLPEVLIGILPGGGGTQRLPRLIGPKAALEMIVTGR
                     HVPAAEAKALGIIDAIVPGKDLLAEAIAFAKSIADKRPLPRVRDKTDKLAEAMADPGM
                     FEAMRKSIARRARGQIAPFNCIAAVEAATKLPFTEGIAEERRLFNELENSDQARGLRY
                     AFFAEREVARIPWLKKDLKLPKIASMAVVGAGTMGGGIAMSFADAGMPVKLMDLSKES
                     LERGLARIRDNYATSVKRGSLPQAEMDKRMALITPADSYEAIGDCDAVIEAVFEQMPV
                     KKEVFRSLDKAMKPGALLLTNTSALDIDEIAAVTKRPEAVAGTHYFVPANVMKLFEVV
                     NAAKTAPETLAAAMQLGRDIGKISVYAGNCDGFVANRSRIPFGVEQNLMIEEGALPEQ
                     IDKVFVDFGYPVGPFAVNDMSGLDIGYDTRKRRAAADPKFRKLPIPDKLVEMGRKGLK
                     TGAGWYRYEKGDRTPIADPEVARLIKEVAAEHGITQRAFSDEEIRNRILFSSINEACK
                     IIEEGKALRASDVDVMWLHGFGFPRWRGGLMFWGDEIGAREIYNQVAAWHQRYGARWK
                     PSELLREIAEAGGKLREAKAKAMG"
BASE COUNT         1744 a         3510 c         3449 g         1782 t
ORIGIN      
        1 gtgacgggct cgccagcgtg agcaacgcga ctcgatcgcc gacatgcagc cgatcaagcc
       61 gccggttttc acgaagcagg agcaggcaac ctacgatcgc tgcgagcgct tcctgtgggc
      121 cgtgcgctgc cacttgcact tcctgacggg ccgcgcggag gagcgcctct cgttcgatgt
      181 gcagccggag ctcgcgagcc gcctcggtta tgcgacgcgg cgcggctcaa tgcccgtcga
      241 gcgcttcatg aagcactact tcctgatcgc caaggacgtc ggcgacctta ccgccgtgct
      301 ctgctcggca ctcgaggtcg cccagctcaa gacgtcgccg ggactgggct cgctgctcaa
      361 tccgctcacc tggcgtatgc gccggcgcgt gcgtgcgacg agcgatttcc gggttgacaa
      421 cggccggctc aatgtcgccg acgagaaggt gttcgagcgc gatccggcga acctcctgcg
      481 catatttgcc catgccgagc atgcgcagac gtttctgcat ccggatgcga tcaggctcat
      541 ccgccgcagc gtgccgacgc ttggtccgcg cctgcgcatg agcgacgatg ccaaccgcat
      601 cttcctcgag ctcttgacga gcaagcgggg ctcgcccgag gctgcactcc ggcgcatgaa
      661 cgaggcgggc gtgctcggcc gcttcatgcc tgagttcggc aaggtcgtgt cgatgatgca
      721 attcaacatg tatcatcatt tcacggtgga cgagcacctc gttcgcacgg tcggccaact
      781 ctacgagatc gaacagggtg cgctcgccaa ggagctgccg ctctcgaccc ggatcttctc
      841 ctcgatccag ggccggcggg cgctgttcgt tgccggcttc ctgcatgaca tcggcaaggg
      901 gcggcccgag gaccactcga tcctcggtgc gcgtattgcc cgcacgatct gtcctcgcct
      961 cggcctcacg gacgcggaga cggataccat cgtctggctc atcgagcagc acctcttcat
     1021 gagcaacacg gcattgagcc gcgatctcgc cgacccgaag accattcgcg atttcgccga
     1081 cgtcgtgcag acgccggaaa gactgaagct cctcctcttg ctgaccgtcg ccgacatccg
     1141 tgcggtcggt cccggcacct ggaatggctg gaagggccag ctgctgcgca cgctctacgc
     1201 cgagaccgag ctgcagctca ccggcggcca cacgatgtcg agtcacaagg cgcgcgtcgg
     1261 cgccgcccag gacgaattgc gcaccgcgct cgcagcctgg ccgaaggatg atgtcgaggc
     1321 gttcatcgat cgccactatc cggactactg gctgaagtcg gacgtgcgac gccagatgga
     1381 ccacgccgaa ctcatgcggc gcgcggccaa gacgggaaaa tcgctggcga ccgaatgccg
     1441 gacggacgag ttcacggcga tcaccgagct cacggtgctc gcgccgaacc acccgcggct
     1501 cctctcgctc ttcgccggcg catgtgcgca cgccggcgca aacatcgtcg gcgccaacat
     1561 ctcgacgacg cgcgacgggc tggcgctcga tacgttccag ctgcagcgcg agaacatgga
     1621 ggccgacgag cgccgccgcg ccgaccgcat cgcggacacc atcggccgtc tgctcgaggg
     1681 caaggcgcgc atcggccagc tgatggcgaa gcgccggccg gtcgagaagc gtgtcgccgc
     1741 cttcgcagtg acgcccgagg tgatgatcga caacgcgctc tcggaccggc tgactgtgat
     1801 ccaggtctcg ggacgcgacc ggacggggct cctttacgag ctgacgagca agctctccga
     1861 cctcaatctc gacatcgcct cggcacacat cgcgaccttc ggcgagaagg cggtcgacgt
     1921 gttctacgtg accgacctca ccggaaagaa gatcatgggc gaggcgcgcc agcggctcat
     1981 catcgacggc ctcgcttcgg tgctcgcttc gcacggcggg ctcgacgcgc tgcccgagcc
     2041 tgcggccgcc ccggtgcagg cctgagcgtc acctgcgact gcgagcatgc cgatttcgag
     2101 cacactgcct tgcaacgtcg cgcgaacccg gctaaatgat gtgcggggca tccgccggcc
     2161 gccccgtaag gcacgtcgcc caagagccgc ctcccaatgg ttatggaggc ccgccttgag
     2221 cgactttttc atttctgtcg acgagctcag cgcacagatc gcgacgtcaa ttgcgccgat
     2281 catcatcgat tgccgccgtc gccccaagtt cgacgaggac ccccggaccc tgccgacggc
     2341 gcgttggcgc gaccacactg aagtctcaaa ctgggcggca caagttccgc acggcgcaag
     2401 cgtcgtcctc gcctgcattc atggtcacaa tgtcagccag ctcgcggcgt cgctgctgcg
     2461 tgagcgcggc atcgatgcgc gtgcgctcgc cggcggcatc gaggcgtgga aggcgcgcgg
     2521 cctgccgacg ctcttaaagt ccgcgctgcc tggccgctcg gaggagcagc cgagccgctg
     2581 ggtcacgcgc atccgcccca agatcgatcg tatcgcctgt ccctggctca tccgccgctt
     2641 catcgatgcc gacgccgaat tcctgttcgt cgagccggac tttgtcgccg atgtcgccaa
     2701 ggaggtcggc ggaattccct acgatatcga tggtgtcgaa ttcagccatg aaggtccgct
     2761 ctgcaccttc gatacgatga tcaagcgctt cgggctttca gatccggcgc tcgatgccct
     2821 ggccctcatc gtccgtggcg ccgatacggc gcggctcgat ctcgctcccg aggcggcggg
     2881 cttgctcgcc gtctcgctcg gcaattcgtc gctcgctggc ggcgatgatc gccgcgcgct
     2941 gacgctcggt ttcccggttt atgatgcgct ctatgcctgg gcgcggcatg cgaaaggcga
     3001 aacgcacaac tggccggcca aatcggcatg atcggggcca tgcgcaatct tggttttggg
     3061 ggagcacatg tcgacaactg acagcggcgc aacgccgacc tatgccgagg ccttccgcgt
     3121 ctggctgaag atcggcatcc tctcgttcgg cggacccgcc gggcagatcg ccatgatgca
     3181 ccgcgagctt gtccaagagc gccggtggat cgacgagcag cgctacctga acgcgctcaa
     3241 tttctgcatg ctgctgcccg ggcccgaggc aacgcagctt gccacctatg tcggctggcg
     3301 gctgcatggg gtgattggcg gcatcacggc ggggctcctg ttcgtgctgc ccggcgcgat
     3361 tctcatgctg ttgctgtcgc tactctatgt cgtctacggc aaggtgccca tcggagaagc
     3421 cttgttcgtc ggcatcaagg ccgccgtact cgccatcgtg gtcgaggcgc tcttgcggat
     3481 tgcaaggcgc ggcctcaagg gcagcgccga caaatggatc gcggggctcg ccttcgtcgc
     3541 gatcttcttc ctcgccatcc ctttcccggc gatcgtggcg gcggcggcga tcttcggctt
     3601 ctggcgggcg cgaggcgcgc cggcttcagc cgccgcggcg cccgtcagcg tgccgctctc
     3661 gcgcactttt cgcacggcac tcctctggct cgcgatctgg atcgtgccgc ttgttgccgt
     3721 cgccatgatc ttcgggaaga cgcacgtctt gagccaactc gcctggtact tctcctacct
     3781 tgcctgcttc accttcggcg gcgcctacgc agtgctcgcc tggatggcgg atgacgcagt
     3841 gcgctactac ggctggctgt cgcccggtca aatgatcgat ggcctcggcc tcgccgagac
     3901 gacgccgggt ccgctcattc tcgtcacgca gttcgtcggg tttctcgccg gctatcaggc
     3961 cgccgggcat ggccctgcgc tcgtgctcgc ggtcgccggc gcgctggtca cgctctgggc
     4021 gacgttcgcc ccctgttatc tctggatctt cgtcggcgcg ccctatgtcg agcgcctcag
     4081 tgccgagccg cggctcaagt cggcgctcgc cgccgtgacc gcggccgttg tcggcgtgat
     4141 cctctacctc acggtctggt tcgcgctgca cgtcctgttc aagattgtgc gcgagccatc
     4201 gattgccgcc ggacctctga agctgcgtgc gttcgtcccc gacggggcga cggccgacgt
     4261 ccgcgccgta attcttgccg cggccgcgtt cgtgctggtc tttgcgctcc accgcagcct
     4321 gatcgcgacg cttgccgcct gtggcggtgc ggcactcgcc tggcattgga tcgggtgagc
     4381 gccgcgcgtc gtggcgttca ccgtgtcttc gggatctcga agccggcggc cgcctcgacg
     4441 tgcctgacga tggtcgtgag atcgtcctgg ccggcgccct tgaagacggc gtgcttgaag
     4501 atgaggcgcg ccgcctgcga gacccacatc ggcacgccga gcgcctcgcc ctgcgcgatg
     4561 gcgagctcga cgtccttcag gatcgtgtcc atggtggcgc cgaaggcgaa gctgcgatcc
     4621 atgagtgaga ttgggaactt gtgcagcgtc gcgctgttgc ggcccgaccc tgcgttgatc
     4681 gcagcgagca tcacttccgg atcgagtccg cctttggctc ccatcacgaa cgcttccgcc
     4741 gaggcgacca gcgccacgcc cgagaggatg ttgttggtga gcttcaggag ctgcgccgcg
     4801 cctggcttgt cgccggcgac ggtcacctgc ttcccccaga gcgagacgat cgggcggacc
     4861 ttcgcgaccg ccgccggatc gcccgagagc atcaccgaga ggatgccgtc acgggcgccg
     4921 ggcgggccgc cgctgatcgg gcaatcgacg agcgtgatgc ctttggcggc cagccccgtg
     4981 gcgatcttct cgacgagcgc gatgccgacc gtgcaggtgt tgacgacgat gcgaacagcc
     5041 ttgccatgga tgagaccgct ctcgcccagg catacggcct cgaggctcgg cagcgtcggc
     5101 agcgaggcaa tcgcgatctc gcattgatcg ccgacctcgc gcgggctcgc cgccgggcgg
     5161 gcctgccgtg cggtcagagg agcaagccgc tcagcgtgga gatcgtggat gatcagcgca
     5221 tggccgccgt cgatgagttt ttccgccata ggcaagccca tgttgccgat cccgacgaac
     5281 ccgatggcca tgtctcgccc cttcagtgcg catgatctcg cggatgcccc tgattagcac
     5341 gcgcctgaca ttgcggtgcc gccattccgg gctggcaacc atgcacagac ggcaagcacc
     5401 ttgcgcgatc ggtcccggcc catcactctc agcccaaccg agggagggtg cctatgatca
     5461 gaacgcgcaa gatcggcgat gccacggtga cgagcgtcat cgagtactcg gggccgacgc
     5521 atgccccgga attcctgttc gagaaggatc tcgaccgggc gcggctcaag gcgaacgagc
     5581 gctggcttgc gccgaaccac tacgtgccga agatggaccg cttcatcatc accatacagc
     5641 tctggattct gaaggccggc ggcaacacga ttattgtcga tacgggcgtt ggcaacctga
     5701 agccgcgtgc ggccgagcgc atgaacatgc tcaatacgct ggtactgccc tggctcgagg
     5761 ccgccggcgc cgggccgaac gaggtgacgc acgtcgtcca cacccacatg cacacggacc
     5821 acgtgggctg ggacaccaca ctcaaagccg ggacatggac gccgacattc ccgaaggccc
     5881 gctatctcta tccgaagcgc gacttcgacg acctcgcctc agccgccaag gccggcaagc
     5941 ccgatcccgc gttcgctgac agcgtcatgc cggtggtcga ggcgggcctc gccgacatga
     6001 tcgacccgga gaaggaggcc gcgggctgcc tcgccgtcga gccggcgcca ggacacagcc
     6061 acggcatggt gacgttccgg ctgcgctcgc gggccgagga ggcgatcttc tcggccgacg
     6121 tcatgcatca cccgattcag gtcgccgagc ccagctggaa cagccgctat tgcgtggcgc
     6181 ccgatcaggc gcgcaaaacg cgggccgaac tcctcgcccg cgccgccgac cgcaacgcct
     6241 tgatcatgcc catgcacttc ggtgccccct actgcggcta catccgccgg cagggaagcg
     6301 ggtatcggtt cgagggggcc ggctggtaga ggcggtgtcg ttgaacgtgc tcgatatcag
     6361 gccaatggcc cgacgtgcgc ggtgaacgct gcgacgttgg cgccgaccct tcctgccgag
     6421 gcgatcacgc cgcgaagcgt ctcgtcgttc agaggtatgc catcacggcg gtaggccgcc
     6481 tcgaaatcgg cttcgatctc gccgggcgaa taaaggcggt caacgccggc cgcccgggag
     6541 ctctttcgaa tttgttccaa aatcctgtcg acgcggctct tgaagcgatc gacatcggtg
     6601 aaggctgcaa tgttgatcgc gagcgcaaac tggccatcga cgccggcgac cgggccgtcg
     6661 accatgtttc cgctttccgt gccataggct gcgccggaca ggaggctcga gagcatgccg
     6721 atggccatgc cgagcccgac gcccttgaac gcgccgatgg gctggatcag cccgtccagc
     6781 gcggcaacgg cgtcggtcgt cggctggcca tgggcgtcat aggcccagcc tgcgggaatg
     6841 ggctcgccct tctgcgcgta gaccctgatc ttgccgtgcg cggtcgcgcc gaaagcgcaa
     6901 tccagcacga aagcggcgcc gtcgctgccc ggaatggcga tgccgatcgg attgatgcca
     6961 acgatcttgt cgcgaccgcc ccagggagcc atggtcggca gcgcgttcgt gccggcgatg
     7021 ccgatcatgt cgtgcgccag cgccattcgt acgtagtagt ccatggttcc ggcgtggtta
     7081 gagccgcgca cggcggcgaa ggcgacattt atctccttcg cacgcgcgat tgcctgctcc
     7141 atcgcaaagc gcatggcgac ctggccgaga ttgttgccgc catcgacgac cagggccgcg
     7201 cccttgtcgg agatcagccg gggcgcgcca cgaggatcga caccttcgtc gaggagcttc
     7261 ttcacgtagt ccgggacgcg aaggacgccg tgagaatgaa ttccgcgcag atcggcactc
     7321 gtcaggctct cggcgactgt cccggcggca ccggcggcaa gtccggcagc gcgaaaaacg
     7381 tcctggacag tcgtcgccaa caacgcgagc gggattcgct tttctgtctc ggcacccgga
     7441 taggtcgcca tgcctgctgc tctttcctat tgtgcgcgaa acggcaggat tggacacgag
     7501 cgcgggcggc ataagaggtt gctcgcgatc caatccagcc tacgcctcag tgcatcggca
     7561 gcttctcggc cttcatggct tcctggacgg cggggcgggc ctggacgcgc ttcatgaagg
     7621 cggtgaggtt cggatagggc gccagatcga tcttcgtcgg cacggtccag cgcagcgtgg
     7681 tgaagagata agcgtcggcc acggtgaagc cagcgcccat gatgtagtcg cgcgaagcga
     7741 gatgagcgtc gagatgggcg aagcgcgtcg cgagacgctc gcgggcgttg gtcttgcctt
     7801 cctcgggcat gcccggcgtg aacagtggcg agaagccctt gtgcacctcg gtcgagagga
     7861 aattgagcca cgactgcagc ttgtagcgct cgatggtgcc gttcgccgga gcgaggcgct
     7921 tctccggtgc cttgtcggcg aggtactgta cgatcgccgg accctcggtc aggatggtgc
     7981 cgtcgtcgag ctcgagcgcc ggcacgtagc cgagcgggtt caccgccttg tagtcgccgc
     8041 cgccctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcgtgacgg cgagccccgc
     8101 ctcgcgcgcg acgatgtgcg gggagagcga gcacacaccg ggggcatagt agagcttcat
     8161 gatagcctcg ttgctttcta ttcgggcgcg accgtagtct cggatgaggc accgggcaag
     8221 gttcgtccgc ggaacgactg gtccgcagtc cactccgggc tggccgcgca ctttcccttg
     8281 ccgccgcgcg cgaaaatgtc caccctttcg tgacaagcat cgacagcccg gggaacgctc
     8341 atgtccgata ccgagaagcc gctcgtccgc tacgaggccc gcgatggcat cggcatcatc
     8401 acgattgaca atccgcccgt gaatgcgctc gggcccgggg tctcggacgg catcatctca
     8461 gccgtcgaga aggccgaggc cgacccggcc gtgaaagcga tggtgatgat cggcgcgggg
     8521 cggagcttca tcgcgggcgc cgacatccgc cagttcggca agaaccggcc gaccccgaag
     8581 aagcgcacct acgacgtgct cgacgagacg aagaagccga tcgtcgcggc gatccacggc
     8641 tatgcactcg gcggcggcct cgagaatgca ctcgcctgcc actatcgcgt cgcggtgccg
     8701 agcgcgaagg tcggcctccc cgaggtgctg atcggcatcc tgccgggcgg cggcggcacg
     8761 cagcgcctgc cgcgcctcat cggcccgaaa gccgcgctgg agatgatcgt cacgggccga
     8821 cacgttccag cggcggaagc gaaagctctc ggcatcattg atgcgatcgt gccggggaag
     8881 gacctgctcg ccgaggcgat cgccttcgcg aagtcgatcg cggacaagag accgctgccg
     8941 cgtgtgcgcg acaagaccga caagctcgcc gaggcgatgg ccgatcccgg catgttcgag
     9001 gcgatgcgca aatcgatcgc acggcgtgcc cgagggcaga tcgcgccctt caattgcatt
     9061 gcggcggtcg aggccgcgac caagctcccc ttcaccgagg gcatagccga ggagcgccgc
     9121 ctcttcaacg agctcgagaa ctcggaccag gcgcgcggcc tgcgctacgc tttcttcgcc
     9181 gagcgcgagg tcgctcgcat cccctggctc aagaaggacc tgaagcttcc gaagattgcc
     9241 agcatggccg tcgtcggcgc gggcaccatg ggcggcggca tcgccatgag ctttgcggac
     9301 gccggcatgc ccgtgaagct catggacctc tcgaaagagt cgctcgaacg tgggctggcc
     9361 cgcatccgcg acaactacgc gacgagcgtg aagcgcggct cgctcccgca ggccgagatg
     9421 gacaagcgca tggcgctcat cacgccggcc gacagctacg aggcgatcgg cgattgcgac
     9481 gctgtgatcg aggccgtgtt cgagcagatg ccggtgaaga aggaggtctt caggagcctc
     9541 gacaaggcca tgaagccggg ggcgctgctc ctcacgaaca cgagtgcgct cgacatcgac
     9601 gagatcgctg cggtgacgaa gcggccggag gcagtggcgg gtacgcacta tttcgtgccg
     9661 gcgaacgtca tgaagctctt cgaggtggtg aatgccgcga aaacggctcc cgagacgctc
     9721 gccgcagcca tgcagctcgg ccgcgacatc ggcaagatca gcgtctacgc cggaaactgc
     9781 gacggcttcg tcgcgaaccg gagccgcatc cccttcggtg tcgagcagaa cctgatgatc
     9841 gaggagggcg cgctgccgga gcagatcgac aaggtgttcg tcgacttcgg ctatccggtc
     9901 gggccgttcg cggtcaacga catgtccggc ctcgacatcg gctatgacac acggaagcgc
     9961 cgcgcggcgg cggacccgaa attccgcaag ctgccgatcc ccgacaagct cgtcgagatg
    10021 gggcgcaagg gcctcaagac cggcgccggc tggtacaggt acgagaaggg cgacaggacg
    10081 ccgatcgccg acccggaggt cgcgcgcctc atcaaggagg tggcggctga acacggcatc
    10141 acgcagcgcg ccttcagcga cgaggagatc cgcaaccgca tcctgttctc ctcgatcaac
    10201 gaggcctgca agatcatcga ggaggggaag gcgctgcgcg caagcgacgt cgatgtgatg
    10261 tggctgcacg gcttcggctt cccgcgctgg cgcggcggcc tcatgttctg gggcgacgag
    10321 atcggcgcgc gcgaaatcta caatcaggtc gccgcctggc atcagcgcta cggcgctcgc
    10381 tggaaaccct cggagctcct ccgcgagatc gccgaggccg gcggcaagct gcgcgaggcc
    10441 aaggcgaagg cgatggggtg aggggcctgc ctggcggcgc gcgtcgttcg cagcaggctc
    10501 tcaaaggagg ccgggttc
//
LOCUS       DAORNH010000147        10457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4736_length_10457_cov_2.572967, whole genome shotgun sequence.
ACCESSION   DAORNH010000147 DAORNH010000000
VERSION     DAORNH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4736_length_10457_cov_2.572967"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>314)
                     /locus_tag="PK264_14680"
     CDS             complement(<1..>314)
                     /locus_tag="PK264_14680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD77152.1"
                     /translation="VHAEGIDAEAEQSGWIQPVHSPGRMKIAERRVKQWGAVGAPVAL
                     VDREAMRSLLGSDAWHGGWTNPTGGHINPLALSRGLARVVAAKGGRIFGGTPAERYER
                     RG"
     assembly_gap    315..346
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<347..648)
                     /locus_tag="PK264_14685"
     CDS             complement(<347..648)
                     /locus_tag="PK264_14685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD77153.1"
                     /translation="MTTTQPAMPPSLWAAVTPAGAAFLPLEGELSVDVVVIGAGFTGL
                     STALHLRRMGVDVAVIEAAEPGFGASGRNNGQVIPTLSRPDPAEIVTDPARDPRLVR"
     gene            complement(798..>999)
                     /locus_tag="PK264_14690"
     CDS             complement(798..>999)
                     /locus_tag="PK264_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77154.1"
                     /translation="AVDQFDRNLAHARRFAGRHQGLARWLACRHEGFAGRLARWNPGS
                     EGHHASGSDSSAREEVSSRGAA"
     assembly_gap    1000..1009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1010..1430)
                     /locus_tag="PK264_14695"
     CDS             complement(<1010..1430)
                     /locus_tag="PK264_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77155.1"
                     /translation="MTKLIRWGMGASTALMAFTLALPGIAQTPPPAPTTPPPKVVPGP
                     AVKPAPATAPKSGTPAPTATTAGAPKPTSAASKPAAAAPKTAPKPKTACNKMADEAAC
                     KADTTCQWIAAEMLKDKTGAIVKGPDGTFWAFATSVVG"
     gene            2243..3256
                     /locus_tag="PK264_14700"
     CDS             2243..3256
                     /locus_tag="PK264_14700"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class I"
                     /protein_id="HRD77156.1"
                     /translation="MSMSLEQVAAAMVAPGKGILAADESSGTIKKRFDSIKTENTEPN
                     RRDYREMLFRSSDAMRNHISGVILFDETIRQKGKDGTPLTKIITDAGSLPGIKVDKGT
                     KPLQFCPGEVVTEGLDGLPDRVKEYTGLGARFAKWRAVIDIGAGIPSYTCINANSQAL
                     ARYAAICVEGGLVPIVDPEILMDGDHTIERCAEATEWVLKDVYQQLFYARVPLEKTVL
                     KPNMVIAGKKCAKQASREQVAAETVKILKRCVPTAVPGIAFLSGGQSDEDATAHLSYM
                     NALGPLPWKLSFSYGRALQAAALKAWGGENTAAGQRAFSHRAKMNGFASLGKWKPELE
                     KAA"
     gene            3256..4029
                     /locus_tag="PK264_14705"
     CDS             3256..4029
                     /locus_tag="PK264_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRD77157.1"
                     /translation="MAKVLVERKGPVTTVILNRPEVRNAVDNETAEGLAEAFRAFEAD
                     EEARVAVLWGAGGGFCAGADLKAVASGERLKRYKLDGDGPMGPSRLKLTKPTIAAVSG
                     YAVAGGLELAIWCDLRVVEEDATFGVFCRRWGVPLIDGGTVRLPRLIGQSRALDLILT
                     GRPVGAEEALAMGLANRVVARGKAREVAEALAAEIAVFPQLCMRLDRASVYRQWELPF
                     EHAMRAEFAAGVEAVKAEGQSGAARFVGGAGRGGTFERS"
     gene            4035..4910
                     /locus_tag="PK264_14710"
     CDS             4035..4910
                     /locus_tag="PK264_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD77158.1"
                     /translation="MSSASVPVEHHYADLGDVMLHYVTAGSGPPLVLIHGWPQTWYEW
                     RAVLPELSRHFTCIMPDMRGLGDSSRPLTGYDKKTVSNDIWRVVSERLGHKSFDLVGH
                     DWGGPTAFALASAHQDAVRRLAIIDVVIPGDGGDFSQGGKRWHHQFHITPDLPEALTA
                     GRERIYLGWFYRTFSYKPGWITEADIDEYVRTYSQPGAMRSGFNIYRALDQDAADNRA
                     FLAKEKLRMPVLAVGGACGIPHPFGRGRGTEPEESLRRCATNVTGLVIPECGHFIPEE
                     QPQALTEALLKFFKA"
     gene            4951..>5611
                     /locus_tag="PK264_14715"
     CDS             4951..>5611
                     /locus_tag="PK264_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRD77159.1"
                     /translation="MATPTLTALEIDRYKRHLVLREVGGQGQQKLKAARVLVIGAGGL
                     GSPLILYLAAAGVGTIGVVDDDQVSLDNLQRQIVHDTASVGVAKVASAAATVARINPH
                     VVIEPHAYRIDAGNALDLIGRYDIVADGSDNFATRYLVSDACHLARKPLVFAAVGPFD
                     GYLSTFKPYEAGSDGKPKPTYRCLFPEAPPAGTVANCAEVGVLGSVVGVVGTMQATEV
                     LK"
     assembly_gap    5612..5631
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5632..5787
                     /locus_tag="PK264_14720"
     CDS             <5632..5787
                     /locus_tag="PK264_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase"
                     /protein_id="HRD77160.1"
                     /translation="NGRLVMYDALATRFDQIRFAWDPENPLSGRNPTIRDLSIHAGAG
                     NAAICAA"
     gene            5816..6529
                     /locus_tag="PK264_14725"
     CDS             5816..6529
                     /locus_tag="PK264_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRD77161.1"
                     /translation="MGIRAILFDKDGTIIDYWRTWVPINREVALFAANGDTALAEELL
                     RAGGHDPATDRVTPGSVLAAGSVDDIAGAFRTRLGSRAPSRLEREIDRIFSEGGARHS
                     VLLDGAAAAIAALHARGFMLGIATNDTIGGLEASLPRHDVRRWMVFEAGCDSGHGGKP
                     APGMVTAFAAAAGVALAEIAVVGDAVHDLEMARAAGAGLKVAVLSGTSPRETLAPHAD
                     LVLVSIADMPGHPAFAAVG"
     gene            complement(6540..7754)
                     /locus_tag="PK264_14730"
     CDS             complement(6540..7754)
                     /locus_tag="PK264_14730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD77162.1"
                     /translation="MTTKTTAAASDGALSGVKVVDLSRVLGGPFGTQLLADHGADVIK
                     VEPPQGDEVRDWGPPFDEGDASYFIGINRNKRSIGLDLGKPEGRAVLMKLLDGADILV
                     ENFKPGSMEAWGIGYAELEKRFPRLIYCKISGFGAEGPLGGFPGYDGIIGAMTGHYSV
                     NGAPETGPIRLGLPVVDISTGLYSVIGILMALHERGRSGRGQFLDMTLYDSALSLMHP
                     HFPNYYLTGKVPGLTGNTHGNLAPYGMFPTKTCRVVIGAGNNRAYARLITELGHPELA
                     EDPRFKTNGDRVKNRAALNEIIVAALSKHDGVALSLKLLAAGLPCGPVLDTGQVLANE
                     HVKVRRMVVEKDGYKGTGMPIKMSRTPAKLRSRPPRFSEHARQVLAEHGFSEEQMAAL
                     AKAGVLVEKRRS"
     gene            complement(7775..8290)
                     /locus_tag="PK264_14735"
     CDS             complement(7775..8290)
                     /locus_tag="PK264_14735"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRD77163.1"
                     /translation="MPRVDDIPEGYRPCVGVMILNRAGLVWVGHRADDGFKLRDQENW
                     WQMPQGGIDAGETPRAAAMRELHEETGIASGTIIAETDGWLRYDLPPELVGHAWKGRW
                     KGQAQRWFAIRFTGADSEIDIAAKPGHTAEFDSWRWVPMADLVGLIVPFKRDVYREVV
                     SAFSHLAGTMA"
     gene            complement(8357..9475)
                     /locus_tag="PK264_14740"
     CDS             complement(8357..9475)
                     /locus_tag="PK264_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD77164.1"
                     /translation="MSLTKKLKRRQFLKGAAVVGAGVGTSAIVTPAISQGKVEWRMVT
                     TWPKNFPGQGTGAVRFAESVGRATDGRLTIKVFAAGEIVPPFESFDAITRGAADCMHA
                     TPYYWPNKSKGLMFFSTVPFGLTTSEQEAWMQFGGGQELWDKIYDGFGLIGWHAGSTG
                     VQMGGWFNKEITTVDSVKGMKMRIPGLGGEALKKLGMTVVNLPGGEIFAALQSGAIDA
                     TEWVGPYNDLAFGFYKVAKFYYWPGMHEPGTQIEFTADKKKFLALPKDVQDILRACIA
                     RENVTFTAEFNARNGQALETLIKTHNVTLKRFPNEFLTAYGAAAGEVIQELRDTGDAN
                     TKAVVESFLKARRELLAWNRIAEQGFLNARLLDYKFPG"
     gene            complement(9928..10152)
                     /locus_tag="PK264_14745"
     CDS             complement(9928..10152)
                     /locus_tag="PK264_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77165.1"
                     /translation="MTTNELIDALATRAKRLSEDRRREIAAALHEMLAEPYRLSADEI
                     AVLEPALKEALAGDALTAADADELLTKPWS"
     gene            complement(10230..>10457)
                     /locus_tag="PK264_14750"
     CDS             complement(10230..>10457)
                     /locus_tag="PK264_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD77166.1"
                     /translation="NLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSV
                     TRERIRQIEAKALRKLKHPSRSRKLRSFLDN"
BASE COUNT         1703 a         3344 c         3619 g         1729 t
ORIGIN      
        1 cgccgcgccg ctcgtagcgc tcggcgggtg tcccgccgaa gatgcggccg cccttggcgg
       61 cgacgacgcg ggcgagaccg cggctcaagg cgagcggatt gatgtggccg ccggtcgggt
      121 tggtccagcc gccgtgccag gcgtccgagc cgaggagtga ccgcatcgcc tcgcgatcga
      181 cgagggcgac cggtgcaccg accgcgcccc actgcttgac gcgccgctcg gcgatcttca
      241 tgcggcccgg cgaatggacc ggctggatcc atcccgattg ctcggcttcg gcatcgatgc
      301 cctcggcgtg cacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga ccagccgcgg
      361 gtcgcgggca ggatcggtga cgatctcggc gggatccggg cgtgagagcg tcgggatcac
      421 ctggccgttg ttgcggccgg aggcgccgaa gcccggctcg gcggcctcga tcacggcgac
      481 gtcgacgccc atgcgtctca gatggagcgc ggtcgagagg ccggtgaagc cggcgccgat
      541 cacgacgacg tcgacagaaa gttcaccctc gagcggcagg aatgccgcgc cggctggtgt
      601 gacggcggcc caaagcgagg gcggcatggc tggctgggtg gtggtcatga ggggccgggc
      661 atcggttgga gtgcgacgat tatagcgcac ggcggcggca ggagtgatcc atcgctgcgg
      721 cccaagcgat cgcggaatgg acagttgctc aaaatcaaac cgggccgcaa tcgtggcgat
      781 tgcgacccgg tcgacagtca cgcagccccg cgtgagctta cttcttcgcg ggcggagctg
      841 tcggagccgg aggcgtggtg gccttcggag ccggggttcc agcgggcgag ccggccggcg
      901 aacccttcgt ggcggcaggc gagccagcgg gcgagccctt ggtggcggcc ggcgaaccgg
      961 cgggcgtgcg cgagattccg atcgaactga tccaccgcan nnnnnnnnnt tccgacaacg
     1021 ctggtggcga atgcccagaa ggtgccgtcc ggacctttca cgatcgcgcc agtcttatcc
     1081 ttgagcattt cggcggcgat ccactggcag gtcgtgtctg ccttgcaagc ggcctcatcg
     1141 gccatcttgt tgcacgccgt cttcggcttc ggcgccgtct tgggggcggc ggcagccggc
     1201 tttgaggcgg cagaagtcgg cttcggagcc ccagccgtgg tcgccgtcgg tgcgggcgtg
     1261 ccggacttcg gcgcagtggc cggtgccggc ttgactgcag gacccggaac caccttcggg
     1321 ggcggtgtcg tcggggccgg aggcggcgtc tgtgcaatac cgggcagcgc gagcgtgaac
     1381 gccatcaagg cggtcgaggc gcccatgccc cagcgaatga gtttggtcac ggcagtcatc
     1441 tccaggtggt tgatgccacg gatcagtata gcacccatgc gtccgacaaa aacacggcgt
     1501 tgaagtgaac aagtgttcac aacgcgacag ttctttcggt aaggtgtgtc cggctggcac
     1561 cagcacagga catcgacaag gctggcgagc agagctgcac gacgcgcggc ctcagtccgt
     1621 gcagcccgcg ctggtgaaag aaccggttct gaaatcacga ttagaatccg gcacttcgat
     1681 ggcggccatc ggcatcgtcg cacgacatcc gggcagctgc cacgcgagcg cgaactcgat
     1741 atcggccaac ctcgggcgcg ggatgctctg ccgaagttgc ccgtcgtgcc gatcgtcgcc
     1801 gccgcagctc gctcgaattg tggaaaacgc agggcggaag tctggacggg gccagaaggg
     1861 cgtcgaatcg gttggttgcg aagaccggaa aaaaggaccg gtgacgagct cgcgccaggg
     1921 agcagggagg aacgcgcgaa ctcgtcaccg gcgcagtctc ggacgcatgt cgatcagtcg
     1981 ccggcggacg ggggaggccg ggacgagggg tccgagagtg ctcgagacac cagggccatg
     2041 acggggggca aggctccggt cttgttcctc tggcgcgggg acgccgaaga ggttcaagta
     2101 ccccagagat gcgcggggca gagccatttc ctccacgcac ggcaaccatt caccgccaat
     2161 ctcggctccg ggcctggaaa ggccgagcaa tgagcgctat ggctttgcca ccgcagaggc
     2221 aggtccaaac agggagaaga cgatgtcgat gtcgctcgag caggttgccg cggccatggt
     2281 ggcaccgggc aagggtattc ttgctgccga cgagagctcc ggcacgatca agaagcgttt
     2341 cgactcgatc aagaccgaga acaccgagcc caatcgccgc gactatcgtg agatgttgtt
     2401 ccgctcctcc gatgcgatgc gcaaccatat ctcgggcgtg attctgttcg acgagacgat
     2461 ccgccagaag gggaaggacg gcacgccgct caccaaaatc atcacggacg ccggctcgct
     2521 ccccggtatc aaggtcgaca aggggacgaa gcccctgcag ttctgtccgg gcgaggtcgt
     2581 cacagagggc ctcgacgggc tccccgaccg cgtgaaggag tacacgggcc tcggcgccag
     2641 gttcgccaag tggcgcgcgg tgatcgacat cggcgcgggc attccctcct acacctgcat
     2701 caacgcgaac tcgcaggcgc tcgcgcgcta tgccgcgatc tgcgtcgagg gcggcctcgt
     2761 gccgatcgtc gaccccgaga tcctgatgga cggcgaccac acgatcgagc gctgcgccga
     2821 ggcgaccgag tgggtgctga aggacgtgta ccagcagctc ttctatgcgc gcgtgccgct
     2881 cgagaagacg gtcttgaagc cgaacatggt gatcgccggc aagaagtgcg caaagcaggc
     2941 gagccgcgag caggtcgccg ccgagacggt gaagatcctg aagcgttgcg tgccgacggc
     3001 agtgccgggc atcgctttcc tctctggcgg ccagtccgac gaggatgcga ccgcgcacct
     3061 ctcctacatg aatgcactcg ggccgctccc ctggaagctc tccttcagct acggccgcgc
     3121 gctgcaggcg gcagcgctca aggcatgggg cggcgagaac accgcggccg gccagcgcgc
     3181 cttctcgcac cgcgccaaga tgaacgggtt cgcgagcctc ggcaaatgga agcccgagct
     3241 cgagaaggcg gcctgatggc gaaagtcctc gtcgagcgca aggggccggt gacgaccgtc
     3301 atcctcaacc ggcccgaggt gcgcaacgcc gtcgacaacg agacggcaga aggcctcgcg
     3361 gaggcgttcc gcgcgttcga ggccgacgag gaggcgcgtg tcgccgtgct ctggggtgct
     3421 ggcggcggct tctgcgccgg cgccgacttg aaggcggtcg cttcgggaga gcggctcaag
     3481 cgctacaaac tcgacggtga tggcccgatg gggccgtcgc gcctgaagct cacaaagccg
     3541 acgattgcgg ccgtctccgg ctacgcggtc gccggcgggc tcgagcttgc gatctggtgc
     3601 gacctgcgcg tcgtcgagga ggacgcgacg ttcggcgtct tctgccggcg ctggggtgtg
     3661 ccactgatcg acggcggcac ggtgcgcctg ccgcgcctca tcggccaaag ccgtgcgctc
     3721 gacctgatcc tcaccggtcg gccggtcggc gccgaggagg cgctcgccat gggcctcgcc
     3781 aatcgcgtgg tggcgagggg caaagcgcgc gaggtggcgg aggctttggc cgccgagatc
     3841 gcggtcttcc cgcagctctg catgcggctc gaccgcgcct cggtctatcg ccagtgggag
     3901 ctgccgttcg agcatgccat gcgggcggag ttcgcggccg gtgtcgaagc ggtgaaggcc
     3961 gaggggcagt cgggagcggc ccgcttcgtt ggcggtgccg ggcgcggtgg tacgttcgag
     4021 aggagctgag agacatgagc agcgcgagcg ttccggtcga gcaccactac gccgatctcg
     4081 gcgatgtcat gctccactat gtgacggcag gctccgggcc gccgctggtc ctcatccatg
     4141 gctggccgca gacctggtac gagtggcgcg cggtgctgcc agagctctcg cgtcacttca
     4201 cctgcatcat gccggacatg cgcggcctcg gcgacagctc gcggccgctc acgggctacg
     4261 acaagaagac ggtctcgaac gacatctggc gtgtcgtctc cgagaggctt ggccacaagt
     4321 cgttcgatct cgtcggccac gactggggcg gcccgacggc cttcgcgctg gcgtcggcac
     4381 accaggacgc cgtgcgccgc ctggcgatca tcgacgtggt gatccccggc gacggcggcg
     4441 acttctcaca gggcggcaag cgctggcacc atcagttcca catcacgccc gatctccccg
     4501 aggcgctgac ggcggggcgc gagcgcatct atctcggctg gttctatcgc accttcagct
     4561 acaagccggg ctggatcacc gaggccgata tcgacgagta cgtccgcacc tactcgcagc
     4621 cgggcgcgat gcggtcaggc ttcaacatct accgggcgct cgaccaggac gcggcggaca
     4681 accgggcctt cctcgccaag gagaagctgc gcatgcccgt gctcgccgtc ggcggcgcct
     4741 gcggcatccc gcaccccttc ggccgaggcc gcggcacgga gcccgaggaa agccttcgcc
     4801 gctgcgcaac gaacgtgaca ggccttgtca ttcccgagtg cggccacttt attccggaag
     4861 agcagccgca ggcgctcacc gaggcactgc tcaagttctt caaggcgtag ggcgaaagcc
     4921 gatccgccgc agagcgtgga ccctgacccg atggcgacgc cgacactgac tgccctcgaa
     4981 atcgaccgct acaagcggca tctggtgctg cgcgaggtcg gcgggcaggg gcagcagaag
     5041 ctgaaggccg cgcgcgttct ggtgatcggc gccggcgggc tcggcagccc gctgatcctc
     5101 tatctcgcgg cggcgggcgt cggtacgatc ggcgttgtcg acgatgatca ggtctcgctc
     5161 gacaacctgc agcgccagat cgtccatgac acggcgagtg tcggcgtggc caaggtcgcg
     5221 agtgccgcgg caacggttgc ccgcatcaac ccgcatgtcg tgatcgagcc gcatgcctac
     5281 cggatcgacg ccggcaatgc gctcgatctg atcgggcgct atgacatcgt cgccgacggc
     5341 tccgacaact tcgcgacgcg ctatctcgtg agcgatgcct gccacctcgc gaggaagccg
     5401 ctcgtcttcg cggccgtcgg ccccttcgac ggatatctct cgaccttcaa accctacgag
     5461 gcggggagcg acggaaagcc gaaaccaacg tatcgctgcc tcttccccga ggcgccgccc
     5521 gccggcaccg tcgccaattg cgccgaggtc ggcgtgctcg gctcggtggt cggcgtcgtc
     5581 ggcacgatgc aggcgacgga ggtgctgaag gnnnnnnnnn nnnnnnnnnn naacggtcgc
     5641 ctcgtcatgt atgacgcgct ggcaacgcgg ttcgatcaga tccgcttcgc ctgggatccg
     5701 gagaacccgc tctcgggccg caatccgacc atccgtgatc tctcgataca tgccggcgcg
     5761 gggaatgccg cgatctgcgc tgcctgaacc gatcctcacc tcccggagcc gacccttggg
     5821 cattcgcgcg atcctcttcg acaaggacgg cacgattatc gactactggc ggacgtgggt
     5881 accgatcaat cgtgaggtgg cgctgttcgc ggccaacggc gataccgcac tcgctgagga
     5941 gttgctccgc gctggcggcc acgaccccgc gacagatcgc gtgacgccgg gctccgtgct
     6001 cgccgccggc agcgtagacg acattgccgg cgcatttcgc acgcgactcg ggtcacgcgc
     6061 gccgagccgc cttgagcgcg agatcgatcg catcttcagc gagggtggcg cccgtcattc
     6121 ggtgctgctc gacggtgcgg cggcggcgat cgcggcgctc catgcccgcg gcttcatgct
     6181 cggcattgcg acgaacgaca cgatcggcgg cctcgaggcc tctctccccc gccacgatgt
     6241 ccgacgctgg atggtattcg aggccggctg cgactctggt cacggcggca agcccgcacc
     6301 tgggatggtg accgcgttcg ccgcggcggc cggcgtcgcg ctcgccgaga tcgccgtggt
     6361 cggggatgcc gtccacgatc tcgagatggc gcgggccgcg ggcgccggcc tcaaggtcgc
     6421 ggtcctctcc gggacgagcc cccgcgagac actcgccccg cacgccgatc tcgtgctcgt
     6481 ctcgattgcc gacatgccgg ggcatccggc cttcgcagcg gtcggctgag cggcgcgggt
     6541 cagctccgcc gcttctcgac gagcacgccg gccttggcga gcgccgccat ctgttcctcg
     6601 gagaagccgt gctcggcgag cacctggcgc gcatgctcgg aaaagcgcgg cgggcgcgag
     6661 cgcagcttgg ccggcgtgcg cgacatcttg atcggcatgc cggtgccctt gtagccgtcc
     6721 ttctcaacga ccatccggcg taccttgacg tgctcgttgg cgagcacctg gcccgtgtcg
     6781 agcacggggc cgcaggggag gcccgccgcg agcagcttca gcgagagtgc gacgccgtcg
     6841 tgcttggaca aggccgcgac gatgatctcg ttgagggccg cgcgattctt gacgcggtcg
     6901 ccgttggtct tgaagcgcgg gtcctcggcg agttcgggat ggccgagctc cgtgatgagg
     6961 cgggcgtacg cgcggttgtt gcctgcgccg atcacgaccc ggcaggtctt ggttgggaac
     7021 atgccgtagg gcgcgaggtt gccgtgcgtg ttgccggtga ggcccggcac tttccccgtc
     7081 agatagtagt tcgggaaatg cgggtgcatg agcgacaagg cgctgtcgta gagcgtcatg
     7141 tcgaggaact gcccgcggcc cgagcggcct cgctcgtgca aggccatcag gatgccgatc
     7201 acggagtaga gcccggtcga gatgtcgacg acagggaggc ccaagcggat cgggccggtc
     7261 tcgggcgcac cgttcacgct gtagtggccg gtcatggcgc cgatgatgcc gtcgtagccc
     7321 ggaaatccgc cgagcgggcc ctcggcgccg aagcccgaga tcttgcaata gatgagtcgg
     7381 ggaaagcgct tctcgagctc ggcatagccg atgccccagg cttccatgga gcccggcttg
     7441 aagttctcga cgaggatgtc ggcgccgtcg aggagcttca tgagcacggc gcggccctcg
     7501 ggcttgccga gatcgaggcc gatcgagcgc ttgttccggt tgatgccgat gaagtaggag
     7561 gcatcgccct cgtcgaaggg agggccccag tcgcgcacct cgtcgccctg cggcggctcg
     7621 accttgatga cgtcggcgcc gtgatcggca aggagctgcg tcccgaacgg gccgccgagc
     7681 acgcgcgaga ggtcgaccac cttgacgccc gagagcgccc catcgcttgc ggctgcagtc
     7741 gtcttcgtcg tcatgtcgat ctccggatgt ggcctcaggc catggtgccg gcgaggtgtg
     7801 agaaggcact caccacctcg cggtagacgt cgcgcttgaa gggcacgatg aggccgacga
     7861 gatcagccat tgggacccac cgccagctgt cgaactcggc cgtgtggccg ggtttggcgg
     7921 cgatgtcgat ctcgctgtcg gcgcctgtga agcggatcgc gaaccagcgc tgagcctggc
     7981 ctttccagcg gcctttccag gcatggccga cgagctcggg cgggaggtcg tagcggagcc
     8041 aaccatcggt ctcggcaatg atggtgccgc tggcaatgcc ggtttcctcg tgaagctctc
     8101 gcatggcggc tgcacgtggc gtctcaccgg catcgatgcc gccctgcggc atctgccacc
     8161 aattctcctg gtccctgagc ttgaagccgt cgtcggcgcg atgcccgacc cagacgaggc
     8221 ccgcccggtt caggatcatg acgccgacac agggacgata gccttcggga atgtcatcaa
     8281 cgcgcggcac cgggatctcc atgcggccgt ggcgcctacg cgaaaagccc gcggacggag
     8341 gtccgcgggc cgaggttcag ccggggaact tgtagtcgag aaggcgggcg ttgaggaagc
     8401 cctgctcggc aattctgttc caggcgagca gctcgcggcg cgccttgagg aagctctcga
     8461 cgacggcctt ggtattggca tcgcccgtat cgcggagctc ctggatcacc tcgccggcgg
     8521 cggcaccgta ggcggtcagg aactcgttcg ggaagcgctt caaggtgacg ttgtgcgtct
     8581 tgatgagcgt ctcgagcgcc tggccgttac gggcgttgaa ctcggccgtg aaggtgacgt
     8641 tctcgcgggc gatgcaggcg cgcaggatgt cctgcacgtc cttcggcagc gccaggaact
     8701 tcttcttgtc ggccgtgaac tcgatctgcg tgccgggctc gtgcataccg ggccagtagt
     8761 agaacttggc gaccttgtag aagccgaagg cgaggtcgtt gtaggggccg acccattccg
     8821 ttgcgtcgat ggcgcccgac tggagggctg cgaagatctc gccacccggc aggttcacga
     8881 ctgtcatgcc gagcttctta agggcttcac ccccgaggcc cgggatacgc atcttcatgc
     8941 ccttcacgct gtcgacggtg gtgatctcct tgttgaacca tccgcccatc tgcacgccgg
     9001 tcgagcctgc atgccagccg atgaggccga agccgtcgta gatcttatcc cagagctcct
     9061 gcccgcctcc gaactgcatc caggcctcct gctcggaggt cgtgaggccg aacggcacgg
     9121 tcgaaaagaa catcaggccc ttgctcttgt tgggccagta gtagggcgtc gcgtgcatgc
     9181 agtcggcagc accccgcgtg atggcatcga agctctcgaa cggaggcacg atctcgcctg
     9241 cggcaaatac tttgatcgtc aagcggccgt cggtcgcgcg tccgacgctc tcggcgaagc
     9301 ggacagcacc ggtgccctgg ccggggaagt tcttcggcca ggtggtcacc atcctccatt
     9361 cgaccttgcc ctgcgagatt gcgggcgtga cgatggcgga cgtaccgacg cccgcgccga
     9421 cgacggcggc gcccttcaag aactggcgac gcttgagttt cttcgtgaga gacatgcgga
     9481 aggctccctt gatcactggc gacagtgtag tgcggacggt atggcccgca cgctatgatg
     9541 tggcgccgtc tcgcggtcgc cacctgcaac cggcatggag agtaggccgg ggtggccgcg
     9601 ttttgcaatg cgtgtgcgcg agatcatgcc atcacggtgc gaggtccgtg aggtcaggga
     9661 gcttccggga tgtgtgccgg atatgcagca ccacgatcgc ggtctcggtg accgtgaagt
     9721 atatacggta gggatacctc gtaggagcga ggactcggat cgctgggtgg ctgctctgga
     9781 ccccgatttc gggaaagtgg gcaagtcgct cgagccgaga gcgcaggtgt tgcctcaccc
     9841 ggtcagccgc gagaggctcg ccactctcga gcaaatgacg gcggatgact tcgagatccg
     9901 aacgcgcccg ccggtgcaac agcaccttca tgaccagggt ttggtcagca actcgtcggc
     9961 atcggcggct gtgagggcat caccagcgag cgcctccttc agtgctggct caagaacggc
    10021 aatttcatcg gctgacagac gatatggctc tgccagcatc tcgtgcaggg ccgctgcgat
    10081 ctcgcgccgt cgatcttctg acagtcgttt ggcgcgagtg gcaagcgcat cgatgagttc
    10141 gttcgtggtc attcgacgat gatagtctcg ccggggccaa aggtgcaatg gcaagcgcgg
    10201 cgtacccgag ggatccgcaa acgccaccct caattgtcga ggaagctccg cagcttcctt
    10261 gagcggctcg ggtgcttgag cttgcgcagc gccttcgcct cgatctgacg gatgcgctcg
    10321 cgggtgaccg agaactgctg gccgacctct tcgagtgtgt ggtcagtatt cataccgatg
    10381 ccgaagcgca tgcggagcac gcgctcctcg cgcggcgtca gcgaggccag cacacgggtc
    10441 gtcgtctcgc gcaggtt
//
LOCUS       DAORNH010000148        10427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4754_length_10427_cov_2.689549, whole genome shotgun sequence.
ACCESSION   DAORNH010000148 DAORNH010000000
VERSION     DAORNH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4754_length_10427_cov_2.689549"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PK264_14755"
     CDS             <1..593
                     /locus_tag="PK264_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HRD77167.1"
                     /translation="NNGLTDFKDILGFPMQSERTRLVLYMVSVTTLLVLFLFAHAMVT
                     SSFGKVLIAIRDAESRTRFLGYRVEHYKLVVFVVSAMMAGLAGALYVPLVGIINPSEF
                     LPSASIEAVIWVAVGGRGTLVGAAVGAVVVNFMKTIFTTGALAAFWPFLLGAVFVVVT
                     LLLPKGIVGTLADLGSSLGRRNGSATPGGLVAKPAE"
     gene            601..1386
                     /gene="urtD"
                     /locus_tag="PK264_14760"
     CDS             601..1386
                     /gene="urtD"
                     /locus_tag="PK264_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HRD77168.1"
                     /translation="MSDKIETDLPAADHITEALLYLDGVSVSFDGFKAINSLSLVIEP
                     GEMRAIIGPNGAGKTTMMDIITGKTRPDVGDVLFDGGAVDLTRLDEAAIANLGIGRKF
                     QKPTVFESQTVADNILLAIKGPRGPFASLWGARNRIPASTIDAILETVRLLDHRARPA
                     GDLSHGQKQWLEIGMLLAQEPKLLLVDEPVAGMTDAETETTAELLREINKERSVIVVE
                     HDMAFVRALGVKVTCLHEGSVLAEGSIDAVSAEPRVVEVYLGR"
     gene            1614..2309
                     /gene="urtE"
                     /locus_tag="PK264_14765"
     CDS             1614..2309
                     /gene="urtE"
                     /locus_tag="PK264_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HRD77169.1"
                     /translation="MLSVEGIDLAYGAAQVLRNVSLAVPIGAVTTVLGRNGVGKTSLL
                     RAIAGHQPIRSGRIVWMGEDIHSLAPFERARRGIALVPQGREIFPLLSVQENLETGFA
                     PLARASRSIPAEIFDLFPVLQSMLGRRGGDLSGGQQQQLAIARALVTRPKLLILDEPT
                     EGIQPSIIKDIGRAILHLRGRGDMAVLLVEQYFDFAKELADHFAVMDRGQIVLAGTKA
                     AMDETDVRRRLAV"
     gene            2284..3162
                     /locus_tag="PK264_14770"
     CDS             2284..3162
                     /locus_tag="PK264_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HRD77170.1"
                     /translation="MSAVASLSESREVRDGAAAIALAPVRQIGGVDIRFARRGATTVA
                     MDRAEAGGYRVRTPSSEFGAEAVLMNTGGGLVGGDRAEQRIALHDDAVALVTTSAAER
                     VYRSLGPPARVAVDLTVAEHARLAWLPQETILYDGARLERTIDASIAGSGTLLAAEVT
                     ALGRRASGEQMRSGAIMDRWRIRRDGRLAYADSYVIDGALDAMMARRAVGAGASVFGT
                     LLYLAHDAETQLDAVRAGIGAWVATAGTCRAAASAWNGMLVMRLQGSNLASLRALIVR
                     VVPRLLGRPMPRNWWS"
     gene            3172..3474
                     /locus_tag="PK264_14775"
     CDS             3172..3474
                     /locus_tag="PK264_14775"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HRD77171.1"
                     /translation="MNLTPREKDKLLIAMAAIVARRRLQRGVKLNHPEAIALITDFVV
                     EGARDGCSVAELMRDGAKVLTREQVMPGIAEMIHDVQVEATFPDGTKLVTVHNPIR"
     gene            3501..4085
                     /locus_tag="PK264_14780"
     CDS             3501..4085
                     /locus_tag="PK264_14780"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HRD77172.1"
                     /translation="MTKHFLRAATPLVLITLLSDPANAHTGLETGFSLVDGILHPLSG
                     FDHVAAMVCVGLWAALAGGRAQWMLPASFFGAMVAGAVMARLGVVPPSIETGIAVSVI
                     GLGLAVAAGEKVPMSLAALVVAIFGLFHGAAHGAEAPSGSFTGYAVGFALTTMVLHAS
                     GFAIARLIERFTSQEPVRAIGALAAASGLVLVLR"
     gene            4088..4438
                     /locus_tag="PK264_14785"
     CDS             4088..4438
                     /locus_tag="PK264_14785"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HRD77173.1"
                     /translation="MIPGEIIAAQGEIELNAGRATLTIDVANTGDRPIQVGSHYHFFE
                     TNPALVFDREKTRGFRLDIAAGTAVRFEPGQTREVRLVALAGKREVYGFRQAVMGKLD
                     GKSRGTKASKRRSS"
     gene            4603..4887
                     /locus_tag="PK264_14790"
     CDS             4603..4887
                     /locus_tag="PK264_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRD77174.1"
                     /translation="MTKMATMTSKGQITVPAEVRADLQLETGDKILFERDEHGTYRIA
                     ARKRPSLVDSARQRPIRSNVSVDDLDRLIQDSVAEAMRGKSRRATRGARR"
     gene            4884..5279
                     /locus_tag="PK264_14795"
     CDS             4884..5279
                     /locus_tag="PK264_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRD77175.1"
                     /translation="MIGIATNVLLRLFLADVADQLRRAQAGFRTASEEGPVHINAIVL
                     SEFAWTLAKGFKLSRAKVAVHRQDVLEADDIDVMLRPAAEAALASYRKGKADFFDYNL
                     AGINAAVGCTTTLTFDRDALDHSQFSPVP"
     gene            5289..7004
                     /gene="ureC"
                     /locus_tag="PK264_14800"
     CDS             5289..7004
                     /gene="ureC"
                     /locus_tag="PK264_14800"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HRD77176.1"
                     /translation="MPAKISRAAYAQMFGPTTGDKVRLADTELVIEVERDLTIYGEEV
                     KFGGGKVIRDGMGQSQVTSAKGAVDTVITNALILDHTGIYKADIGIKAGRIARIGKAG
                     NPDIQPGVDIIVGPGTDVIAGEGKIVTAGGFDSHIHFICPQQIDDALMAGMTTLLGGG
                     TGPSHGTFATTCTPGPWHIQMMLRAIDAVPVNIGIAAKGNTSLPAPLIEMVEAGACAL
                     KLHEDWGTTPAAIDNCLSVADDHDVQVMLHSDTLNESGFVEDTIAAFKGRTIHAFHTE
                     GAGGGHAPDIIRIAGLANVLPSSTNPTRPFTVNTLDEHLDMLMVCHHLSPSIPEDLAF
                     AESRIRKETIAAEDILHDLGALSMMSSDSQAMGRIGEVITRTWQTAHKMKVQRGPLPE
                     DRGKGNDNFRARRYVAKYTINPAIAHGVARHIGSVEAGKLADLVVWSPAFFGVKPDCV
                     IKGGMIVAAPMGDPNASIPTPQPVHYRPMFGAYGGAVAATALTFTSQAAVSAGLSRKL
                     GLKKQLVAVENVRGGISKKSMILNDATPDISVDPETYEVRADGELLVCEPAKVLPMAQ
                     RYFLF"
     gene            7058..7354
                     /locus_tag="PK264_14805"
     CDS             7058..7354
                     /locus_tag="PK264_14805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HRD77177.1"
                     /translation="MIYVIATMTLKPGTREKVLAAAKPCIAATRKEKGCIFYDLNVST
                     DNPDLLTFVERWETREDLAAHAKSEHIKVWRAASGDHVASRMIEIITPSSVDKM"
     gene            7363..7929
                     /locus_tag="PK264_14810"
     CDS             7363..7929
                     /locus_tag="PK264_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HRD77178.1"
                     /translation="MPSVTRVAPRGSAAWGTAVDTITLDRQSRYRRRMAMTSDGGRRF
                     LLDLAEATYLEAGDRLLLDDGSAIEVRSADEQLLEITASDRHALMRIAWHIGNRHTPC
                     EITDAALFIQVDHVLAAMVEGLGGTARAVMRPFEPEGGAYGGKGALEHGHHHHDHGDG
                     HSHHHHEHEHAEANGSSRRPSVWRPDGA"
     gene            7919..8662
                     /locus_tag="PK264_14815"
     CDS             7919..8662
                     /locus_tag="PK264_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HRD77179.1"
                     /translation="MALDPPSASAPNSAVGHALPPEQLLNVWLSPAFPVGSFAYSHGL
                     ECAVETGLVADAAGLRDWLGDLVSHGSAWNDLVLASIAMRATSERAWPSLAGAAEIAL
                     ALQPSAERHLETSQQGGSFLDQILAAWRHPVIDQALMGLHDGDRREPIAYPIAFAIAA
                     GAHGVPIRASLIALGLAFAGNLVSAAIRLSVLGQTDGQRVIASLSEEIASAADRAASA
                     SEDELGSMTLVSDLMSIEHETQRTRLFRS"
     gene            8659..8883
                     /locus_tag="PK264_14820"
                     /pseudo
     CDS             8659..8883
                     /locus_tag="PK264_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927794.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     assembly_gap    8884..8896
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8897..9313
                     /gene="ureG"
                     /locus_tag="PK264_14825"
     CDS             <8897..9313
                     /gene="ureG"
                     /locus_tag="PK264_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HRD77180.1"
                     /translation="VETGGCPHTAIREDCSINLAAISTMRKRFPDLDLILIESGGDNL
                     AATFSPELADITIYVIDVAQGEKIPRKGGPGVTRSDLLVINKTDLAPHVGARLDVMAS
                     DAARQRGERPVVFACMQTGEGVVEIARFIEEAGGLA"
     gene            complement(9344..>10427)
                     /locus_tag="PK264_14830"
     CDS             complement(9344..>10427)
                     /locus_tag="PK264_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD77181.1"
                     /translation="QDMEFVQFHPTGIYGAGVLITEGSRGEGGYLTNANGERFMERYA
                     PHVKDLASRDVVSRSMTVEIREGRGVGPEKDHIYLHLDHLDPKILAERLPGITESAKV
                     FAGVDLRRQPIPVLPTVHYNMGGIPTNYHGEVLTLKNGNPDTVVPGLMAIGEAACVSV
                     HGANRLGSNSLIDLVVFGRAAGLRAASTTEKGKAHATLPSSSIDFALSRLDRFRHAKG
                     GTPTAALRLKMQKVMQTNCAVFRDGPVLTEGVNGIMEIWKGSGDIGVTDRSLIWNSDL
                     VETLEYDNLIAQAAVTMAGAENRKESRGAHAREDFPNRDDKEWMKHTLAWADTTAHGV
                     FIDYRPVHTNTLTNEVKYIEPKARVY"
BASE COUNT         1787 a         3414 c         3390 g         1823 t
ORIGIN      
        1 gcaacaacgg cctcaccgat ttcaaggaca tcctcggctt cccgatgcag tcggagcgca
       61 cgcgcctcgt gctctacatg gtgagcgtca cgacgctgct cgtgctcttc ctcttcgcgc
      121 acgccatggt gacgtcgtca ttcggcaagg tactgatcgc catccgagac gcggagagcc
      181 ggacgcgctt cctgggttac cgggtcgagc actacaagct cgttgtgttc gtcgtctcgg
      241 ccatgatggc gggcctcgcg ggcgcgctct acgtgccgct ggtcggaatc atcaatccgt
      301 cggagttcct gccgtcagcc tcgatcgagg cggtgatctg ggtcgcagtc ggcggccgcg
      361 gcacgctcgt cggcgcggcg gtcggcgcgg tcgtcgtcaa cttcatgaag acgatcttca
      421 cgacgggagc gctcgccgcg ttctggccgt tcctgctcgg tgccgtgttc gtggtcgtga
      481 cgctgctgct gccgaagggc atcgtcggca ctctcgcaga cttgggctcg agcctcggcc
      541 ggcgcaatgg atcagcgacg cccggcggtc tcgtcgccaa gcccgcggag tgaggtgaac
      601 atgagcgaca agatcgagac agacctgccg gccgccgacc acatcaccga ggcgcttctc
      661 tatctggacg gcgtgagcgt ctctttcgac ggcttcaagg cgatcaacag tctctcgctc
      721 gtcatcgagc cgggcgagat gcgtgccatc atcggcccga acggggccgg caagacgacc
      781 atgatggaca tcatcaccgg caagacacga cccgatgtcg gcgacgtcct cttcgacggc
      841 ggcgcggtcg acctcacacg cctcgacgaa gcagccatcg cgaaccttgg catcgggcgg
      901 aagttccaga aaccgaccgt cttcgagagc cagaccgtcg ctgacaacat cctcctcgca
      961 atcaaggggc cgcgcggacc attcgcatcg ctctggggcg ctcgcaaccg cattcccgcc
     1021 tcgaccatcg acgccattct tgagaccgtc cgccttctcg atcaccgcgc gcgccctgcg
     1081 ggcgatctca gccacggcca gaagcagtgg ctcgagatcg gcatgctgct cgcgcaggag
     1141 ccgaagctgc tactcgtcga cgagccggtc gccggtatga ccgacgcgga gaccgagacg
     1201 actgcggaac tgcttcgcga gatcaacaag gagcgctcgg tgatcgttgt cgagcacgac
     1261 atggccttcg tgcgcgcgct cggggtcaag gtgacgtgcc tgcacgaagg cagcgtgctc
     1321 gccgaaggct cgatcgatgc cgtctctgca gaaccgcggg tcgtcgaggt gtacctcggc
     1381 cgatgacccg cgcaagatcc atagcccgtc atatcgaaag tcccgtgcat gcgacgcttg
     1441 ccgccacatc tgctgcaccg accgacccgg gccgggcgcg cccacgtcat tccggcgtgc
     1501 cggtcgacga ccgtgacgaa ccctggtccg gtgtcgagcg cgcgcaagag cgttcgtggc
     1561 gcttcgagat cgctccgcaa agagctggtc gtcctggcta gggcgccgcg cgaatgctga
     1621 gcgtcgaggg tatcgatctc gcttacgggg cggcgcaagt gctgcgcaac gtgagcctcg
     1681 ccgtgccgat cggggccgtg acgaccgtgc tcggccgcaa cggcgtcggt aagaccagct
     1741 tactgcgcgc catcgccgga catcaaccga tcaggtcggg ccggatcgtg tggatgggcg
     1801 aggacatcca ctcgcttgcg cctttcgagc gggcgaggcg ggggatcgcg ctcgtgccgc
     1861 agggacgcga aatcttcccg ctgctctcgg tccaagagaa cctcgagacg ggttttgccc
     1921 cgctggcccg cgcaagccgc tccatccctg ccgagatttt cgatctcttc cctgtcctac
     1981 agagcatgct cggccggcgt ggcggcgacc tctccggcgg ccagcagcag cagctcgcga
     2041 tcgcccgggc cctcgtcacg cggccgaagc tcttgattct cgatgaaccg accgagggca
     2101 tccagccgtc catcatcaag gacatcgggc gggcaatcct gcacctgcgc ggccgtggcg
     2161 acatggccgt cctgctcgtc gagcagtact tcgattttgc caaggagctt gcggaccatt
     2221 tcgcggtgat ggatcgtggc cagatcgtgc tcgccggcac gaaggccgcc atggatgaaa
     2281 ccgatgtccg ccgtcgcctc gctgtctgag tcgcgagaag tgcgagatgg cgctgccgcc
     2341 atcgccctgg cgccggtccg ccagatcggc ggtgtcgaca tccgctttgc gcggcgcggc
     2401 gcgacgacgg ttgccatgga tcgtgccgaa gccggcggct atcgcgtgcg cacgccctcg
     2461 agcgagtttg gcgccgaggc cgtgctgatg aacaccggcg gcggcctcgt cggcggcgac
     2521 cgcgccgagc agcggatcgc gctacatgat gacgccgtgg cactggtgac cacgtcagcc
     2581 gccgagcgtg tctaccgatc gctcggtccc ccggcgcgcg ttgccgtcga tctcactgtc
     2641 gcggagcacg cgcgtctcgc ctggctgccg caggagacga tcctttacga tggcgcgcgt
     2701 ctcgagcgca cgatcgacgc tagcatcgcg ggatcaggaa cgctgctcgc ggccgaggtc
     2761 acggcgctcg ggcggcgcgc ctcgggcgag cagatgagat cgggcgcaat catggatcgc
     2821 tggcgcatcc gtcgggacgg ccgcctcgcc tacgccgact cgtacgtgat cgatggagcg
     2881 ttggatgcga tgatggcgcg cagggccgtc ggcgctggcg ccagcgtctt cggtactctc
     2941 ctatatctgg cgcacgacgc ggaaacgcag ctcgatgcag tccgtgccgg catcggggca
     3001 tgggtcgcga ctgcgggtac atgccgtgct gccgcgagcg cctggaatgg gatgctggtc
     3061 atgcgtctgc aaggctcgaa cctggcatct ctgcgcgcgc tgatcgttcg cgtggtgccc
     3121 cgcctgctcg gacgcccgat gccacgaaac tggtggagct gagtcgccgc tatgaacctc
     3181 acccctcgcg aaaaagacaa gctgctcatt gccatggccg cgatcgtggc gcggcgccgc
     3241 ctccagcgcg gcgtgaagct caatcatccc gaggcgatcg cgctgatcac cgatttcgtc
     3301 gtcgagggcg cgcgcgacgg ctgctcggtc gccgagctga tgcgcgacgg cgcaaaggtg
     3361 ctcacgcgcg agcaggtgat gccgggcatc gccgagatga tccacgatgt gcaggtcgag
     3421 gcgacattcc ccgacggcac caagctcgtc accgtccaca acccgatccg ctgacagtca
     3481 atggtaggca aggaaacgtc atgacaaagc acttcctccg ggcggcaacg ccgctcgtat
     3541 tgatcacgtt gctgagcgat cctgccaacg cgcacaccgg gcttgagacc ggcttttcgc
     3601 tggtcgatgg catcctgcat ccgctgagcg gcttcgacca tgtcgccgcc atggtgtgcg
     3661 tcggtctttg ggcagcactc gcaggcggtc gggcgcaatg gatgcttccg gcgtcatttt
     3721 tcggagccat ggtggccggg gcagtcatgg caagactagg tgtggtgccg ccaagtatcg
     3781 agacaggcat tgcagtgtcc gtgattggcc ttggactcgc tgtcgccgct ggcgaaaagg
     3841 tgccaatgtc gcttgccgcc ttagtcgttg ccatcttcgg cctattccac ggggcggctc
     3901 atggcgccga ggctccgagt ggcagtttca ctggatatgc tgtgggcttt gccctgacca
     3961 cgatggtcct tcatgcctct ggtttcgcca ttgcccgcct gatcgagcgc ttcacgagcc
     4021 aggagccggt gcgcgccatt ggcgccctcg ccgccgcttc cgggcttgtt ctcgtactga
     4081 ggtagtcatg atccccggag aaatcatcgc ggcgcagggt gagatcgagc tcaacgctgg
     4141 ccgggcgacg ctgacgattg acgtcgcgaa caccggtgac cggccgatcc aggtcggcag
     4201 ccattaccac ttcttcgaga ctaatccggc tctcgtattc gaccgcgaga agacccgcgg
     4261 tttccgcctc gacattgctg ctggcacagc ggtccgcttc gagccgggcc agacccgcga
     4321 ggtgcgcctg gtcgcactgg ctggaaagcg cgaggtctac ggcttccgcc aggccgtgat
     4381 gggcaagctc gatggcaagt cccgcggaac aaaagcatcc aagcggcggt cgtcctgacc
     4441 aaggtctatt cggacggctc tggtccgcgc tcgcagctgg cctcccttca tgcctcttcg
     4501 ccacaatgtg ggcctcggta tgatcgagca ctggcggaaa ttgatcctgg catatccttg
     4561 cgatggtctt tggtaaggca gacatccagg taagacactg tcatgaccaa gatggcgacg
     4621 atgaccagca agggccaaat cacggtgccg gccgaggtgc gagcggatct tcagctcgaa
     4681 acgggcgaca agatcttgtt cgagcgcgac gaacatggga cctatcggat cgctgcacgc
     4741 aagcgcccca gtctcgtcga cagcgcgagg caacgaccca tccggagcaa cgtgagcgtc
     4801 gacgacctcg atcgcctgat tcaagactct gtcgccgagg ccatgcgcgg caaatcgcgg
     4861 cgggcgacgc gtggggcgcg gcgatgatcg gcatcgcaac aaacgtgctg ctgaggctct
     4921 tcctcgcaga cgttgccgat cagctgcggc gtgcgcaggc gggtttcagg acggcctccg
     4981 aagaggggcc tgtacacatc aatgcgatcg tactgagcga gtttgcctgg actctcgcga
     5041 aagggttcaa gctttcgcgc gccaaggtcg cggtacaccg ccaggacgtg ctcgaggccg
     5101 acgatatcga tgtcatgctt cgacctgccg cggaggctgc attggcctcc taccgcaagg
     5161 gcaaggctga tttttttgac tacaatctcg ccggcatcaa cgcggcagtc ggctgcacaa
     5221 cgacactaac gttcgatcgg gatgcactcg accattccca gttctcaccc gtgccttgag
     5281 gttcgatcat gccggccaaa atctcccgtg ccgcctatgc gcagatgttc ggcccgacga
     5341 ccggcgataa ggtgcgcctc gccgataccg agctcgtcat cgaggtcgag cgtgacctca
     5401 cgatctacgg cgaggaggtg aagttcggcg gcggcaaggt gatccgcgac ggcatgggcc
     5461 agtcgcaggt gacgagcgcc aagggcgcgg tcgataccgt gatcaccaat gcgctcattc
     5521 tcgaccacac aggcatctac aaggccgaca tcggcatcaa ggcggggcgc atcgccagga
     5581 tcgggaaggc cggtaatcct gacatccagc cgggcgtcga catcatcgtc ggcccgggca
     5641 cggatgtcat cgcgggcgaa gggaagatcg tcaccgcggg cggattcgac agccacatcc
     5701 acttcatctg cccgcagcag atcgacgacg cgctgatggc cggcatgacg acgctgctcg
     5761 gtggcggcac cggcccctca cacggaacct tcgcgaccac ctgcactccg ggaccgtggc
     5821 acatccagat gatgttgcgt gcgatcgacg cggtgccggt caacatcggc atcgccgcaa
     5881 aaggcaacac gtcactcccg gccccgctca tcgagatggt cgaggcgggc gcctgtgcct
     5941 tgaagctgca cgaggactgg gggacgacgc cagccgcgat cgacaattgc ctctccgtcg
     6001 ccgacgacca tgacgtacag gtgatgctgc actccgatac gctgaacgag agcggcttcg
     6061 tcgaggacac gatcgcggcg ttcaagggcc gcaccatcca cgccttccac accgaaggtg
     6121 ctggcggcgg acacgcgccc gacatcatca ggatcgcggg gctcgcgaac gtgctgccat
     6181 cctcgaccaa cccgacacgg cccttcaccg tcaacacgct cgacgagcac ctcgacatgc
     6241 tgatggtctg ccatcacctg tcgccgtcga tcccggagga cctcgcgttc gcggaaagcc
     6301 gcatccgcaa ggaaacgatc gcagctgaag acatcctcca cgatctcggc gcgctctcga
     6361 tgatgtcgtc tgacagccag gccatggggc ggatcggcga ggtgatcacg cggacctggc
     6421 agaccgcgca caagatgaag gtgcaacggg gaccgttgcc cgaggatcgc ggcaaaggga
     6481 acgacaactt ccgcgcccgc cgctatgtcg cgaagtacac gatcaacccc gcgatagctc
     6541 acggtgtggc acgccacatc ggctcggtgg aggccggcaa gctcgccgat ctcgtcgtct
     6601 ggtcgccggc tttcttcggc gtgaaacctg actgcgtgat caagggcggc atgatcgtcg
     6661 cggcacccat gggtgatccg aatgcctcca tcccgacgcc gcagcccgtg cactaccggc
     6721 cgatgttcgg cgcatacggc ggcgcggtcg cggcgaccgc gcttacgttc acgtcgcagg
     6781 cggcggttag cgcgggcctc agccgcaagc tcggcctcaa gaaacagctc gtcgcggtcg
     6841 agaacgtgcg cggcggcatc tcgaagaaga gcatgatcct gaatgatgcg acgcccgaca
     6901 tctccgttga tcccgaaacc tacgaggtgc gggccgacgg cgagctgctc gtctgcgagc
     6961 cggccaaggt gctgccgatg gcgcagcgct acttcctgtt ctaggatcgg gcgaggctaa
     7021 cggcctgcac gcaatctcga aatgtgagga atgagccatg atctacgtca tcgccaccat
     7081 gaccctgaag cccggcacgc gcgagaaggt gctggccgcg gccaagcctt gcatcgcggc
     7141 gacgcgcaag gaaaagggct gcatcttcta tgatctgaac gtgagcacgg acaatcccga
     7201 tctcctgacc ttcgtcgagc gctgggagac gcgcgaggac ctcgccgcgc acgcgaagag
     7261 cgaacacatc aaggtgtggc gcgcggcctc gggtgatcac gtggcaagcc gcatgatcga
     7321 gatcatcacg ccgtcaagcg tcgataagat gtgagcatgc ggatgccgag tgtcacgaga
     7381 gttgcgcccc gcggctcggc tgcgtggggc accgccgtcg atacgatcac gctcgaccgg
     7441 caatcgcgct atcgccggcg catggcgatg acgagcgacg ggggccggcg tttcctgctc
     7501 gatcttgccg aggcgacgta tctcgaagcc ggtgaccggc tgttgctcga cgatggctcg
     7561 gcgatcgagg tgcgctcggc ggacgagcaa ctcctggaga tcaccgcttc ggatcgccac
     7621 gcgctcatgc gcatcgcttg gcacatcggc aatcggcaca cgccctgcga gatcacggac
     7681 gccgcgctct ttattcaggt ggaccatgtc ctggccgcga tggtcgaggg tctcggcggc
     7741 acagcacgcg ctgtgatgcg gccattcgag cccgagggcg gtgcctacgg cggcaagggc
     7801 gcgctcgagc acggtcatca ccatcatgat catggcgatg gccacagcca tcatcatcac
     7861 gagcatgagc atgccgaggc gaacggctcg agccgccgcc cgagcgtttg gcggcccgat
     7921 ggcgcttgat ccgccctctg catcagcacc gaactcggcg gtagggcacg cactgccccc
     7981 cgagcagctg cttaatgtct ggctttcgcc ggcttttccc gtcggaagct ttgcctacag
     8041 ccacggcctc gagtgcgctg tcgagaccgg gctcgtcgcc gatgcggcgg gtctgcggga
     8101 ctggctcggc gacctcgttt ctcatggatc ggcctggaac gacctggtgc tcgcctctat
     8161 cgcgatgcgc gccacgtcgg aacgcgcctg gccgtctctg gcgggcgccg cagagatcgc
     8221 cctcgcgctg cagccatcgg ccgagcgcca cctcgaaacg tcgcagcagg gcggctcgtt
     8281 tctcgaccag attctggccg cgtggcgaca cccggtcatt gatcaggcgc ttatggggtt
     8341 gcatgatggc gatcgtcgcg agccgatcgc ctacccgatc gcattcgcca tcgccgcagg
     8401 cgcgcacggc gtgccgattc gcgcttcact catcgcgctc ggacttgctt tcgctggaaa
     8461 tctcgtctcg gcggcgatcc gcctttcggt gctcggccag acggacggac agcgcgtcat
     8521 cgcatcgctg tcggaggaga tcgcctcggc ggccgaccgt gccgcaagcg cgagcgagga
     8581 cgaactcggc tccatgacgc tcgtttcgga cctgatgtcg atcgagcatg aaacccaacg
     8641 aacgaggctg ttccggtcat gacgacgata cctgcagagg cgagcgcaac ggtgcgccgc
     8701 acgcgatccg gtcacggccc gctgcgtgtc ggcatcggcg gtccggtcgg ctccggcaag
     8761 accacgctca ccgagaagct ctgccgcgcc ttccgcgaca agcacgacat atgcgtcatc
     8821 accaacgaca tctacaccaa ggaggatgcc gaaatcctga tgcgggcgca ggccctgccg
     8881 ctcnnnnnnn nnnnnngtcg agaccggcgg ctgcccgcat accgcgatcc gcgaggactg
     8941 ctcgatcaat cttgccgcga tctcgaccat gcgaaagcgc ttccccgatc ttgatctcat
     9001 cttgatcgaa tcgggcggcg acaatctggc ggcgacattc tcgcccgagc tcgccgatat
     9061 tacgatctat gtgatcgacg tcgcgcaggg cgagaagatc ccgcggaaag gcgggccggg
     9121 cgtcacgcgg tccgacctgc tggtcatcaa caagaccgat cttgcgcccc atgtcggcgc
     9181 gcgcctcgac gtgatggcgt ccgacgcagc ccgccaacgt ggcgagcgcc ccgtcgtctt
     9241 cgcctgcatg cagacgggcg agggcgtcgt cgagatcgcc cgcttcatcg aggaggccgg
     9301 cgggctcgcg tgagcgcccg cccgcacctc tcgaaacggg caatcagtac acgcgcgcct
     9361 tcggctcgat gtacttcacc tcgttggtga gcgtgttggt gtggacaggg cggtagtcga
     9421 tgaagacgcc gtgcgccgtg gtgtcggccc aggcgagcgt gtgcttcatc cactccttgt
     9481 cgtcgcgatt ggggaagtcc tcgcgagcgt gcgcgccgcg gctctctttc cggttctcgg
     9541 cgccggccat ggttacggcg gcctgcgcga tgaggttgtc atactcgagc gtctcgacga
     9601 ggtccgagtt ccagatcagc gagcggtctg tgacgccgat gtctcccgaa cccttccaga
     9661 tctccatgat gccgttcacg ccttcggtca gcaccgggcc atcgcggaag acggcgcagt
     9721 tcgtctgcat gaccttctgc atcttcaagc gcaaggcggc ggtcggcgtg ccgcccttgg
     9781 catggcggaa gcgatcgagt cgcgagaggg cgaaatcgat cgagctcgac ggcaaggtcg
     9841 cgtgggcctt tcctttttcg gtggtcgagg cggcgcggag ccccgcggcg cggccgaaca
     9901 cgacgaggtc gatgagcgag ttcgaaccga gccggttggc gccgtggacc gacacacagg
     9961 ccgcctcgcc gatcgccatg aggcccggca cgaccgtgtc cgggttgccg ttcttcagcg
    10021 tgagtacctc gccgtgatag ttcgtcggga tgccgcccat gttgtagtgg accgtcggca
    10081 gcacggggat cggctgacga cggaggtcga cgcccgcaaa caccttggcg ctctcggtga
    10141 tgccggggag ccgctcggcg agaattttcg ggtcaagatg gtcgaggtgg aggtaaatgt
    10201 ggtccttctc cggcccgacg ccgcggcctt cgcggatttc gaccgtcatt gagcgtgaga
    10261 cgacgtcgcg ggaggcgaga tccttcacgt gcggggcata gcgctccatg aagcgctcgc
    10321 cgttcgcgtt tgtgaggtag ccgccctcgc cgcgcgagcc ttcggtgatg agcacgcccg
    10381 cgccgtaaat gccggtcgga tggaactgga cgaattccat gtcctgc
//
LOCUS       DAORNH010000149        10394 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4769_length_10394_cov_2.622497, whole genome shotgun sequence.
ACCESSION   DAORNH010000149 DAORNH010000000
VERSION     DAORNH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4769_length_10394_cov_2.622497"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>301
                     /locus_tag="PK264_14835"
     CDS             <1..>301
                     /locus_tag="PK264_14835"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRD77182.1"
                     /translation="GSGIASIAARLAGAGSVTANDIDPLAGLAARSNAGLNGVAIDIS
                     GEDMLGAQPPAADLVLLADLVYDPEVMTRTTGFLEMARRTGLPVLIGDRTAARRPP"
     assembly_gap    302..353
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            477..1241
                     /gene="truA"
                     /locus_tag="PK264_14840"
     CDS             477..1241
                     /gene="truA"
                     /locus_tag="PK264_14840"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRD77183.1"
                     /translation="MPRYAVIIEYDGTPFVGWQAQAVGRSVQSCLIEAIGRLTGEHVK
                     LQGAGRTDSGVHASGQVAHFDLERAWPAEKIRDGLNYHLRPDPIGIVAAREVGVGFDA
                     RFSAIGRHYRYRILARKAPPILDLNRVWWVPRPLDASLMAEGARHLVGRHDFTTFRAA
                     GCQAASPVKSLDCLEIRRTGDEITIVAGARSFLHHQVRSIVGSLKHVGEGRWVADDIR
                     AALEAKDRTRCGALAPSRGLYLVGVDYPEVALKVAT"
     gene            complement(1234..1686)
                     /locus_tag="PK264_14845"
     CDS             complement(1234..1686)
                     /locus_tag="PK264_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD77184.1"
                     /translation="MPARVAHPLGPDEAFATALARLRAAMADVADGDVSAIKALHSDS
                     DDVTSFYGWGGYEKGAEAVARRWDWAGQQFKGGTVSYQNVSTVVGVDLAYVTDIETFR
                     VKVAGLAAPTEWTNRVTHIFRLENGRWRLLHRHGNRLERQYEPGTRLK"
     gene            complement(1686..2429)
                     /gene="phaR"
                     /locus_tag="PK264_14850"
     CDS             complement(1686..2429)
                     /gene="phaR"
                     /locus_tag="PK264_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HRD77185.1"
                     /translation="MLKRAEAQSDKKEPVVIKKYANRRLYNTETSTYVTLDDLAQMVK
                     GERDFVVYDAKTGDDLTHSVLTQIIMEQESRSEQTLLPVPFLRQLIRFYGGNIEKLVP
                     SYLQFSLDMFTREQERFRSQFSNTFGTSMFEAYQEQAKKNLALFEQAMSMFTPFGPLA
                     GRRPETSSTGSPPTGEPSATPASQPAGTPSAARAAVEFPPFPSVLTSIPTRPATPAAQ
                     PSAQTQELQELKAQLAAMQAKIEKMSGTN"
     assembly_gap    2610..2619
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2733..3920
                     /locus_tag="PK264_14855"
     CDS             2733..3920
                     /locus_tag="PK264_14855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRD77186.1"
                     /translation="MSNDDTTIVIASAARTPVGSFNGAFAALPAHELGKIAIKAALTR
                     AGVEPGDVSEVIMGQILAAGSGQNPARQASINAGIPVDAPAWGMNQLCGSGLRAVALG
                     AQQILDGAKIVVAGGQESMSQAPHVMHLRDGTKMGDTKLIDTMIKDGLWDAFNGYHMG
                     TTAENVARQWQITREEQDRFAVASQNKAEAAKKAGKFKDEIAPVTIKGRKGDTIIAED
                     EYIKDGVTLDGIAKLRPAFDPKEGTVTAANASGINDGAAAIVLMTAAEAKKRGIKPLA
                     RIVSWAHAGVDPSIMGTGPIPASKKALEKAGWTVKDLDLIEANEAFAAQAIAVNKGLG
                     WDTSKVNVNGGAIAIGHPIGASGARVLTTLLHEMKRRDAKKGLATLCIGGGMGVAMCV
                     ARD"
     gene            4031..4759
                     /gene="phbB"
                     /locus_tag="PK264_14860"
     CDS             4031..4759
                     /gene="phbB"
                     /locus_tag="PK264_14860"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HRD77187.1"
                     /translation="MGRVAVVTGGTRGIGQSISLALKAKGYRVAANYAGNDQAARQFQ
                     ADSGIPIYKFDVGDYDACEKAVGQIARDLGPVDVLVNNAGITRDTVLHRMAKHQWADV
                     IRTNLDSVYNMSRLVIDGMRSRNFGRIISISSINGRKGQFGQANYAAAKAGLVGFSKA
                     VALEGAAKGITCNVIAPGYINTEMVAAVPKDVLDTKILPLIPVGRLGTAEEIARCVVF
                     LAADEAGFITGACLDANGGQYMAT"
     assembly_gap    5008..5017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5018..5758
                     /locus_tag="PK264_14865"
     CDS             <5018..5758
                     /locus_tag="PK264_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77188.1"
                     /translation="LAPRHYDPKTGKIILCIQSYVVRTKHHTILVDTCVGNDKHRPHR
                     PFWHLQKAKTYEDGLKSVGLGFDDIDFVTCTHLHVDHVGWNTRLENGRWVPTFKNARY
                     LMSKTELDHWTEREKKDASTCPWITDSVLPIVAANKADLVTSKHVLNDIVHFIPTPGH
                     TIDHFSVRVGRKGADAVIAGDMIHSPIQARYPEIGMMSDYDSAQAGRSRRELFGSLCD
                     TSTLMCTGHFASPSTGHVTRWDDGFRIE"
     gene            5835..6266
                     /locus_tag="PK264_14870"
     CDS             5835..6266
                     /locus_tag="PK264_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77189.1"
                     /translation="MAEALRELAANLAGAEKRRVRFTSIQGLERGDLPLACEVWCDDL
                     YRSPWAERDTMKLATLFVRYICNPNPTAVLMQQIERQCQLARPELVRVLALMKSYGAV
                     DSFMIERDDLRLYLNLTRLQRLRVLEVKHRLAELQSSSGVV"
     gene            6370..6678
                     /locus_tag="PK264_14875"
     CDS             6370..6678
                     /locus_tag="PK264_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77190.1"
                     /translation="MRSMLIAAAVIPALMASSQSALACTPPTVALIESLASLPEPPIP
                     TKCGSDRNEFMRLAHLKDWKGALAAYERHLTSWFGRQEAGTADARATLDYLRRKAAGN
                     "
     gene            6864..8111
                     /locus_tag="PK264_14880"
     CDS             6864..8111
                     /locus_tag="PK264_14880"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRD77191.1"
                     /translation="MAAALPDIDLPTLADVEAARKLIAGHVLVTACSESRTLSEICGA
                     RVWLKQENMQFTAAFKERGAINRLSALTPEERKRGVIAMSAGNHAQGVAYHARRLSIP
                     STIVMPIGTPMVKVENTRRHGAKVIVTGDTLEDAAVFAREHGREKGLTFIHPYDDKLV
                     AAGQGTIALEMLGAAPEIDTLVIPIGGGGLISGMAVAAKALVPTIRIVGVQAQLYPSM
                     YNAIMGTSLPMRGDTLAEGIAVKTPGRLTTEIVRKHVDDIVLVTEADLERAVSLLINI
                     EKTVVEGAGAAGLAALIAHRAHFAGRNVGLVLCGGNIDTRLLASVLTRELARDGRLSQ
                     LAIDLVDRPGQLALVAQVIGEAGANVVEVSHQRVFSDLPAKGALLEVVIETRDRNHRD
                     DAIRLLKERGFNLEVVGNQGGRR"
     gene            8191..8267
                     /locus_tag="PK264_14885"
     tRNA            8191..8267
                     /locus_tag="PK264_14885"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8225..8227,aa:Arg,seq:tct)
     gene            complement(8630..8800)
                     /locus_tag="PK264_14890"
     CDS             complement(8630..8800)
                     /locus_tag="PK264_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRD77192.1"
                     /translation="MRVPAFPEIMTFADDETEALALAKEAIELAIEDRTARGEPIPAQ
                     DAMWLHSVALAA"
     gene            9079..>10017
                     /locus_tag="PK264_14895"
     CDS             9079..>10017
                     /locus_tag="PK264_14895"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRD77193.1"
                     /translation="MHHFDYRDGVLHAEDVSLERIAREVGTPVYVYSAATLERHYTLF
                     AEAVGAPRHKVFYAMKANGNLAVMATLARLGAGIDTVSEGEIRKARAAGVPAERIVFS
                     GVGKSRRELAFAVGEGIYQVNIETESELDMLDLVAQAAGKRQAAVFRVNPDVGDGGNA
                     DITTGSDANKFGVSFADIPRLYARAATLPGVRMLGLAVHIGSLIDRIDSLEAAFKRMA
                     ELVVALRQAGHHVERVDLGGGLAIPYRMSEPFEHGPDLIHEYARMVERVIGPLGVEHG
                     FEPGRIIVGNAGVLLSEVLHLNKRRTKTFAVLDAAMN"
     assembly_gap    10018..10167
                     /estimated_length=150
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10168..10365
                     /locus_tag="PK264_14900"
     CDS             <10168..10365
                     /locus_tag="PK264_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRD77194.1"
                     /translation="ERAPGDLVAFKSAGAYGAAMSSSYNARPLVAEVLVKGGAYSVVR
                     PRQTYEELIGQDRIPAWLDTR"
BASE COUNT         1741 a         3416 c         3338 g         1677 t
ORIGIN      
        1 cgggctcggg catcgcctcg atcgccgctc gccttgccgg tgccggctcc gtgaccgcca
       61 atgacatcga tcctctggcg ggcctcgcgg cgcgatcaaa cgcgggcctg aatggtgtgg
      121 cgatcgatat ctcgggcgag gacatgctcg gtgcgcagcc gcctgcagcc gatctcgtgc
      181 tgctcgccga tctcgtctac gatcccgagg tcatgacgcg gacgacgggg ttcctggaaa
      241 tggcgcgccg tacaggcctc cccgttctga tcggtgaccg cacggcggcg cgccggccgc
      301 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaccact
      361 tcgatcgcgc ccgggtctgg agcctcccga gagcatcgag atcgtgatac gagggcctgt
      421 ccgcacctcc cgctccccgc cagggaccag tccctggatc cgaactgaaa gagccgatgc
      481 cgcgctatgc cgtgatcatc gaatatgacg gcacgccgtt cgtcggctgg caggctcagg
      541 ccgtggggcg gagcgtgcag tcgtgcctga tcgaggcgat cggccgcctc acaggcgagc
      601 acgtgaagct gcagggggca gggcgcacgg actcaggcgt gcacgcgagc gggcaggtcg
      661 cccacttcga tctcgaacgg gcctggccgg cggagaagat ccgcgacggc ctcaattacc
      721 acctgcgccc ggacccgatc gggatcgtcg ccgcgcgcga ggtgggggtc gggttcgacg
      781 cgcgcttttc cgcgatcggc cggcactatc gctaccgtat cctggcgcga aaggcgccgc
      841 cgatcctcga tctgaaccgc gtctggtggg tgccgcgccc gctcgacgcg agcctgatgg
      901 ccgaaggcgc ccgccatctc gtcggccgcc acgacttcac gacgttcagg gcggcgggct
      961 gccaggcggc ctcaccggtg aagtcgctcg attgtctcga gatcaggcgg acaggtgatg
     1021 agatcactat cgtcgccggc gcgcgctcgt tcctccacca tcaggtgcgc tcgatcgtcg
     1081 gcagcctgaa gcacgtcggc gaagggcgct gggtggcgga cgacatccgt gcggcgctcg
     1141 aagcgaagga ccgtacgcgc tgcggcgcgc tcgccccctc tcgcgggcta tatctcgtcg
     1201 gtgtggacta tccggaagtg gcgctcaagg tcgctacttg agccgggttc cgggctcata
     1261 ctggcgctcg agccggttgc cgtggcgatg cagcaaccgc cagcggccgt tctcgagccg
     1321 gaagatgtgc gtcacccggt tcgtccactc ggtcggcgcg gcgaggccag cgaccttcac
     1381 gcggaatgtc tcgatgtcgg tgacgtaggc gaggtcgacg ccgacgacgg tcgacacgtt
     1441 ctgataggag acggtgccgc ccttgaattg ctgcccggcc caatcccagc ggcgcgcgac
     1501 ggcctccgcg cccttctcgt agccgcccca gccgtagaag ctcgtgacgt cgtcgctatc
     1561 ggaatgcaat gccttgatcg ctgagacatc gccgtcggcg acatcggcca tggctgcgcg
     1621 caagcgagcg agcgccgtgg cgaaggcctc atcggggccg agtgggtggg ccacccgggc
     1681 tggcatcagt tggtgccgct catcttctcg atcttcgcct gcatggcggc gagctgcgcc
     1741 ttgagctcct gcagctcctg cgtctgtgcc gagggctggg cggcgggcgt tgctggacgg
     1801 gtcgggatcg aggtcaggac tgagggaaac ggcgggaatt cgactgcagc gcgcgcggct
     1861 gagggggtgc ctgccggctg gctcgcgggc gtcgcagagg gctcgcccgt gggcggcgaa
     1921 ccggtggacg acgtttcggg gcggcgaccg gccagcggtc cgaacggcgt gaacatcgac
     1981 atggcctgct cgaagagcgc gaggttcttc ttggcctgct cctggtaggc ctcgaacatg
     2041 ctggtgccga acgtgttcga gaactggctg cggaagcgct cctgctcgcg cgtgaacatg
     2101 tcgaggctga actgcagata gctcgggacg agcttttcga tgttgccgcc gtagaagcgg
     2161 atgagctggc ggaggaacgg cacgggaagc agcgtctgct ccgagcggct ctcctgctcc
     2221 atgatgatct gggtcagcac cgaatgcgtg aggtcgtcac cggtcttcgc gtcgtagacg
     2281 acgaaatcgc gctcgccctt gaccatctgt gcgaggtcat cgagcgttac gtaggtactc
     2341 gtctcagtat tatagagccg cctgttggcg tatttcttga tgacgaccgg ctctttcttg
     2401 tccgattgag cttctgcccg cttcaacatg gccgtcgcag tcctcagttt tctgcatcgc
     2461 agcataacca atagcacgtg gcttcgccgc tgtgaacggg cgtcgtgctt catcattgag
     2521 cccgcttgat gaatgctcgg agtgcatggc ccactactgt tgttgcaatt gcgtggctga
     2581 tgggcaacga gtaagcgtcg tgccagcagn nnnnnnnnnc gacacacgca ctccgcgccc
     2641 taccagcggc ccatgtggcc gtgacgtcca gggtcccgcc cacggtcgat cgcaggagcg
     2701 atccgcccct cgagacaagg agccacagtc ctatgagcaa cgatgacacg acgatcgtga
     2761 ttgcctctgc cgcgcgcacg ccagtcggct cgttcaacgg cgcattcgct gcgctcccgg
     2821 cacacgagct cggcaagatc gccatcaagg cggcactgac acgcgccggc gtcgagccgg
     2881 gcgacgtgtc cgaggtgatc atgggccaga ttctggcggc gggcagcggc cagaacccgg
     2941 ctcgccaagc ctcgatcaat gccggtattc cggtcgacgc gccggcctgg ggcatgaacc
     3001 agctgtgcgg ctcgggcctt cgcgccgtcg ccctcggcgc ccagcagatc ctcgacggtg
     3061 ccaagatcgt cgtcgccggt ggtcaggaaa gcatgagcca ggcgccgcac gtcatgcatc
     3121 tgcgcgacgg cacaaagatg ggcgatacca agctcatcga cacgatgatc aaggacggcc
     3181 tctgggacgc cttcaacggc tatcacatgg ggacgaccgc cgagaacgtc gcccgccagt
     3241 ggcagatcac gcgcgaggag caggaccgct tcgccgtcgc ctcgcagaac aaggccgagg
     3301 ccgccaagaa ggccggcaag ttcaaggatg agatcgcacc ggtcaccatc aagggccgca
     3361 agggcgacac catcatcgcc gaggacgagt acatcaagga cggtgtgaca ctcgacggca
     3421 ttgcgaagct ccgcccggcc ttcgacccta aggagggcac cgtgacggcg gccaacgcct
     3481 ccggcatcaa cgacggcgcc gcggcgatcg tgctgatgac ggccgcggag gccaagaagc
     3541 gcggcatcaa gccgctcgcc cgcatcgtct cgtgggcgca tgccggcgtc gatccctcga
     3601 tcatgggcac cggtccaatt ccggcctcca agaaggcgct cgagaaagcc gggtggaccg
     3661 tgaaggacct cgacctgatc gaggcgaacg aggcctttgc cgcgcaggcc atcgccgtca
     3721 acaagggcct cggctgggac acgagcaaag tgaacgtcaa cggtggcgcc atcgccattg
     3781 gccatccgat cggcgcctcg ggtgcccgcg tgctgacgac gcttctccac gagatgaagc
     3841 gccgcgacgc caagaagggc ctcgccacgc tctgcatcgg tggcggcatg ggcgtggcca
     3901 tgtgcgtcgc ccgcgactga tcgccgtcat taactttgcc gcccggaagg tgattgccgg
     3961 ccgggcggtg tcgcgcaacg ctgaaagcaa aacgcttcaa tcgaaagagt gagcctgggg
     4021 agggcacacc atgggtcggg tcgcagtcgt cacaggcgga acgcgcggca tcgggcagtc
     4081 gatctcgctc gcactcaagg cgaagggcta tcgcgttgcc gcgaactatg ccggcaacga
     4141 ccaggccgcc cgccagttcc aggccgacag cggcatcccg atctacaagt tcgacgtcgg
     4201 cgactacgac gcctgcgaga aggcggtcgg ccagatcgcg cgcgatctcg gtccggtcga
     4261 cgtgctcgtc aataatgctg gcatcacgcg cgataccgtg ctccaccgca tggcgaagca
     4321 ccagtgggcg gacgtgatcc gcaccaacct cgacagcgtc tacaacatgt cgcgactggt
     4381 gatcgacggc atgcgctcgc gtaatttcgg ccgcatcatc tcgatctcct cgatcaacgg
     4441 ccgcaagggc cagttcggcc aggcgaacta cgcggcggcc aaggcgggcc tcgtcggctt
     4501 ctcgaaggcc gtcgctctcg aaggcgccgc caagggtatc acctgcaacg tcatcgcacc
     4561 cggctacatc aacaccgaga tggtggccgc ggtgccgaag gacgtgctcg acacgaagat
     4621 cctgccgctg atcccggtcg gccgcctcgg cacggccgag gagatcgcgc gctgcgtcgt
     4681 cttccttgcg gcggacgagg ccggcttcat caccggcgcc tgcctcgatg cgaacggcgg
     4741 ccagtacatg gcgacgtgat cgggcgccgc acgagcccga tcgatctctg acgcgcacct
     4801 gccatcgcat ggcgtcgctc gcatccccga cggcgccatg ccatcatggc cgtgagagcg
     4861 ccatagcagg gagcacgcca tgcctcagac catcgagatc ggggacatcc gcatcgaccg
     4921 ggtcatcgag atggaggcgc cgctcttcga cccgctgacg ttcttcccga gcctcggcaa
     4981 ggagcggctc gaggcgaacc tcgattgnnn nnnnnnnctc gcgccccgcc actacgaccc
     5041 gaagacgggc aagatcattc tgtgcatcca gagctacgtg gtgcgcacca agcaccacac
     5101 cattctggtg gatacctgcg tcggaaacga caagcaccgc ccgcaccgcc ccttctggca
     5161 cctccagaag gcgaagacct acgaggacgg cctcaagtcc gtcggcctcg gcttcgacga
     5221 catcgatttc gtcacttgca cgcacctcca cgtcgatcac gtcggctgga acacgcggct
     5281 cgaaaacggg cgctgggtcc cgaccttcaa gaacgcgcgc tacctcatgt cgaagaccga
     5341 gctcgatcat tggacggagc gcgagaagaa ggacgcctcg acctgtccgt ggatcaccga
     5401 ttccgtgctg ccgatcgtcg ccgccaacaa ggccgatctc gtcacgagca aacacgtcct
     5461 caacgatatc gtccacttca ttccgacgcc tggccacacc atcgatcact tctcggtgcg
     5521 cgtcggccgc aagggcgcgg atgccgtcat cgcaggcgac atgatccact cgccgatcca
     5581 ggcgcgctat cccgaaatag gcatgatgtc ggactatgat tcagctcagg ccggccggtc
     5641 gaggcgcgag ctcttcggca gtctctgcga tacctcgacc ttgatgtgca cgggtcactt
     5701 cgcctctccc tcgaccgggc acgtgacgcg ctgggacgac ggcttccgca tcgagtgagc
     5761 gcaaaaccgc cgcgttgaaa cacgatctta tccatcctct ggcatcgtcg agccggtgtg
     5821 gggaggggtg ggtcatggcg gaagcattgc gcgagctcgc ggcgaacctt gccggcgccg
     5881 aaaagcgtcg cgtgcgcttt acctcgatcc aggggctcga gcggggtgac ctgccgctcg
     5941 cctgcgaggt gtggtgcgac gatctctatc gctcgccctg ggccgagcgc gacaccatga
     6001 agctcgcgac gctgttcgtt cgttacatct gcaacccgaa tccaaccgcc gtgctcatgc
     6061 agcagatcga gcggcagtgc cagctcgcga ggccggaact cgtccgcgtg ctcgccctga
     6121 tgaaaagcta cggcgccgtg gacagcttca tgatcgagcg cgacgacctc cgcctctatc
     6181 tcaacctgac gcgcctccag cgcctgcgcg tcctcgaggt gaagcaccga ctggccgagc
     6241 ttcagtcgag ttccggtgtc gtctgagcgt gccgcgagct gtgccggaaa gtgctctcgc
     6301 gagcccaaat cgcatgtgag gcgcgcccga tcggcgtccc gacgtgcgct gcagcgctaa
     6361 gaggccccca tgcgatcgat gctcattgct gccgcagtga tccctgcact catggcgagt
     6421 tcgcagtcgg cactcgcctg cacgccacca accgtcgcgc tgatcgaaag ccttgctagc
     6481 ctgcccgagc cgccaatccc gacaaagtgc ggatcggatc gaaacgaatt catgcggctc
     6541 gcccacctca aggactggaa gggcgcgctg gccgcctacg agcgtcatct cacgtcctgg
     6601 tttggccggc aggaggccgg caccgccgac gcgcgggcga cgctcgacta tctgcgccgc
     6661 aaggcggccg gcaactgaat cgaggcagca cgccatgccc tccaccgccc gccgagggga
     6721 acgcgaggca tgatggcgac gaaatgcggg ttggcgcagc ttctcttcgt gtttacgttg
     6781 cggcgcacat cgtgcgcgtg acgcacccga ggaggcgtgc cttgaccgac ggcatcattg
     6841 agaaacgcgt cacggacggc aagctggccg ccgcgctgcc cgatatcgac ctgccgacgc
     6901 tcgccgacgt ggaggctgcg cggaagctca tcgccggcca tgtgctggtc acggcctgct
     6961 ccgagagccg cacgctctcc gagatatgcg gggcgcgcgt ctggctgaag caggaaaaca
     7021 tgcagttcac cgccgccttc aaggagcgcg gcgcgatcaa ccggctctcc gcattgaccc
     7081 ctgaggagag gaagcgcggc gttatcgcca tgtcggccgg caaccacgcg caaggcgtcg
     7141 cctatcacgc gcggcgcctc tcgatcccct cgaccatcgt gatgccgatc ggcacaccca
     7201 tggtgaaggt cgagaatacg cgccggcacg gcgccaaggt catcgtcaca ggcgacactc
     7261 tcgaggacgc ggcggtcttc gcgcgcgagc acggccgcga gaagggcctc accttcatcc
     7321 acccctacga cgacaagctc gtcgccgcgg gccagggcac gatcgcgctc gagatgctcg
     7381 gcgccgcgcc ggagatcgat acgctcgtca tcccgatcgg cggcggcggc ctcatctccg
     7441 gcatggcggt cgcggccaag gcgctggtgc cgacgatccg gatcgtcggc gtgcaggcgc
     7501 aactctatcc ctcgatgtac aacgcgatca tggggacgag cctgccgatg cgcggcgata
     7561 cgctcgccga aggaatcgcc gtgaagacgc ccgggcggct cacgaccgag atcgtgcgca
     7621 agcatgtcga cgacatcgtg ctcgtcacgg aggccgatct cgagcgcgcg gtgagcctcc
     7681 tcatcaacat cgagaagacg gtggtggagg gcgccggtgc cgcggggctt gcagcgctca
     7741 tcgcgcatcg cgcgcacttc gcgggccgca acgtcggtct cgtcctctgc ggcggcaaca
     7801 tcgatacgcg cctcctcgcg agcgtgctga cgcgcgagct cgcacgcgac gggcgcctct
     7861 cccagctcgc gatcgatctc gtcgaccggc cgggccagct cgcgctcgtc gcccaggtga
     7921 tcggggaggc cggcgccaac gtcgtcgagg tctcgcatca gcgcgtcttc tccgatctgc
     7981 cggcgaaggg cgcgctcctc gaggtcgtga tcgagacgcg cgaccgaaac caccgcgacg
     8041 acgccattcg cctcctcaag gagcgcggct tcaacctgga ggtcgtcggc aaccagggcg
     8101 gccggcgctg aggcgcgcgc gggcgggatg cctggcgcgc cgccgatatc gacgtgtccc
     8161 ttgtcgccgt gctagatcac ccgccggaat gcgcccgtag ctcagccgga tagagcaccc
     8221 gccttctaag cgggtggtcg caggttcgac tcctgccggg cgcgccacaa gcacgattgt
     8281 ccgatagaga ctaggatcgg gcggtgcccg gcgcacgggc aacgcaattc actcgggcct
     8341 gtggcatatt gaccggcgct gccttcgcca acgcgccgcc actcgtcaag gtagccgcca
     8401 caacagtgcg agaaactcat tctcggtaag ccctacctgc tcgatgacat cggagagtgt
     8461 ctcgcgcggg atgtccttag cccttatgca ccgagaccgt ggttgcgcgc gaaggatcgc
     8521 tgctatgcac gggtcgccga tggctccctt tgcagctggt gacgacgaac ccgggcttct
     8581 ccagtgcgcg aatgacctcc gggcctttag tgagggctgc gcctggcatt catgcggcaa
     8641 gcgccactga gtgcagccac atggcatctt gcgccggtat cggctcgccg cgggcggtgc
     8701 gatcctcgat agccagctcg atggcttcct ttgccaaagc gagggcttcg gtctcatcgt
     8761 cggcaaaggt catgatctcc gggaacgcgg gcacgcgcac ggtaaggccc cccttgggct
     8821 cctgcagacg cagaacgtcc aatgctcggg cgacggacgc gtcggagttc cttgagtggc
     8881 acaccgcatg tcgatcatcg tatcactccc cgcacgcttg gtccttttcc tcgcccctcc
     8941 atcgtccctt gccgctcgca gttagcccga ggcccagctg cgcgaggagc caacgggcgc
     9001 cgagattgac ggcgctcggc ggaacgtgtc aggcaagcgt cgcacgatct tcatccgcga
     9061 aaccggccgg gcgctcccat gcatcacttc gattatcgcg acggcgtcct ccacgccgaa
     9121 gacgtgagcc tcgagcggat cgcgcgggag gtcggcaccc ccgtctacgt ctactcggcg
     9181 gcgacgctag agcgccacta cacgcttttc gccgaggccg tcggcgcgcc acgccacaag
     9241 gtcttctacg cgatgaaggc caacggcaat ctcgccgtaa tggcgacgct cgcgcggctc
     9301 ggcgcgggca tcgacacggt gtcggagggc gagatccgca aggcgcgggc cgccggcgtg
     9361 ccggctgaga ggatcgtctt ctccggtgtt ggcaagagcc ggcgcgagct cgcctttgcg
     9421 gtcggcgaag gcatctacca ggtgaacatc gagaccgaga gcgagctcga catgctcgat
     9481 ctggtcgcgc aggcggccgg caagcggcaa gccgccgtat tccgcgtcaa tccggacgtg
     9541 ggcgacggcg gcaacgccga catcacgacg ggctcggatg ccaacaagtt cggcgtgtcg
     9601 ttcgcggata tcccgcgcct ctatgcgcgc gcggcgacct tgccgggcgt gcgcatgctg
     9661 gggctcgccg tgcatatcgg cagcctgatc gatcgcatcg acagcctcga ggctgcgttc
     9721 aagcgcatgg cggagcttgt ggtggcactg aggcaagcgg ggcatcacgt cgagcgcgtc
     9781 gatcttggcg gcggcctcgc gatcccttat cgcatgagcg agccgttcga gcacggtccc
     9841 gatctcatcc acgagtatgc gaggatggtc gagcgcgtca tcggtccatt gggtgtcgag
     9901 cacggcttcg agccgggccg catcatcgtc ggcaatgccg gcgttcttct gagcgaggtc
     9961 ttgcacctca acaagcgccg cacgaagacg tttgcagttc tcgatgcggc catgaacnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnngag cgcgcgccgg gcgatctcgt cgccttcaag
    10201 tcggcaggcg cctacggcgc cgcgatgtcg tcgtcgtaca atgcgcggcc gctcgttgcc
    10261 gaggtgctgg tgaagggcgg ggcgtactca gtcgtccgcc cgcgccagac ttatgaggaa
    10321 ctcatcggcc aggaccgcat tccggcctgg ctcgacacgc gctgaccgcg aggcggccta
    10381 ctgcggcacg gcgg
//
LOCUS       DAORNH010000150        10384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4776_length_10384_cov_2.549134, whole genome shotgun sequence.
ACCESSION   DAORNH010000150 DAORNH010000000
VERSION     DAORNH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_10384_cov_2.549134"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK264_14905"
     CDS             complement(<1..772)
                     /locus_tag="PK264_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77195.1"
                     /translation="MTAMLEARDVSRTFMVSRGFMRGKRPLRAVNGISLSVAQGEVVA
                     LVGESGCGKTTLARMLLGLLAPTSGEIQIDGKPIGSLPRHEVARLVQPVFQDPYSSLN
                     PRKSIGSIIALPLKVQGDPEPATWRRRVEEMMELVGLNRRLYDNYPSQLSGGQRQRVA
                     IARALINRPRMVICDEPTTALDVSVQSQILNLLEELRRSLGLTYLVISHNLAVVEHMA
                     SRVAVMYLGRIVEEAPTDTLFSNPKHPYTEALLSAVPIP"
     gene            complement(769..1785)
                     /locus_tag="PK264_14910"
     CDS             complement(769..1785)
                     /locus_tag="PK264_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD77196.1"
                     /translation="MTERILTVDGLDVKIPVPAGTLHPVQDVSFTVDRGETVCIVGES
                     GCGKSLTALAIMDLLPSRAKRSARRLEIADTDMTRLSERQMSDLRGNRMAMIFQEPMT
                     SLNPAYTIGNQLIEAMRRHKRVSTSDARARAINLLERVGITAAAERLGQYPHQLSGGL
                     RQRVMIAMALMCGPDLIVADEPTTALDVTIQAQILHLLAELQRELRMGLILITHDLGI
                     VARIATRVVVMYAGQVVETGTAEQIFRRPSHPYTRGLLACIPIPGRTKRGDALGTIPG
                     MVPSLVGAIQGCHFASRCPIGDAGCRAAPVALAPPRTTTTTASVHLVRCLKSGAMMPA
                     GATA"
     gene            complement(1880..2806)
                     /locus_tag="PK264_14915"
     CDS             complement(1880..2806)
                     /locus_tag="PK264_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77197.1"
                     /translation="MANVSIGAPAAGMPTAAEILAPSPGALMRRRIVRHWGLMIGSLV
                     LIVMILMALLADVIAPHDPLAQDLERKLIPPIWHASAKATWAHILGTDHLGRDYLSRL
                     IHGARVSLYIGFAAMLISGAIGTTLGVIAGYFGGRVDMVVNFIVTTRLSMPVVLVALA
                     VVSLVGSSLQVVIWVLGLLIWDRFAVVMRSATQQVRSREFITAAEAVGCSTTRILLTE
                     VAPNVMNHLIVVATLEMAHAVLLEAALSFLGLGVQPPTPSWGLMVSEAKNLMFFEAWL
                     IAIPGLALFLLVLSINLLGDGLRDVTAPENRA"
     gene            complement(2808..3758)
                     /locus_tag="PK264_14920"
     CDS             complement(2808..3758)
                     /locus_tag="PK264_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77198.1"
                     /translation="MLVYSLKRLGIAILVALTVSLITFSMIYVSGDPAIAMAGEGARP
                     EDIDNIRKFYGFDRPIPVQYFDWLKGAVTGDLGRSYNLRQPVADVIFSRLPVTMTLAL
                     CAITFALVLAIPLGVVAAVRPNSIVDRTALGIAVLGQAMPSFWFALTLMLWLGIYGLN
                     INLYWWSTRIQLPITGSDSWANFVMPAIALGYYLAPGVMRLTRAGMLDVLASDYIRTA
                     RAKGLRPITVLFKHALRNAIIPVVSLAAVQFGFMLGGSIVIESIFQINGLGYLAWESI
                     QRKDLPMMQAIVLILSMIYILLTFLADLLNAFLDPRIRVA"
     gene            3934..4704
                     /locus_tag="PK264_14925"
     CDS             3934..4704
                     /locus_tag="PK264_14925"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRD77199.1"
                     /translation="MSDMRRSDDSKHLRLGVNIDHVATVRNARGGAHPDPVRAAQAAI
                     EAGADGITAHLREDRRHIRDDDIRRLKAEITRPLNFEMAATEEMARIALATLPNACCL
                     VPEKREERTTEGGLDVVGQGNTLQGYIARLKAKGIRVSLFIEPDLRAVEASARLGADI
                     VELHTGTYCEKALAHDADRVRLEIERIARAATHAKAAGLEVHAGHGLTYLTVGPIARM
                     PEMVELNIGHFLIGEAIFVGLRGAIGEMRRLMDEARAR"
     gene            4701..5108
                     /gene="acpS"
                     /locus_tag="PK264_14930"
     CDS             4701..5108
                     /gene="acpS"
                     /locus_tag="PK264_14930"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRD77200.1"
                     /translation="MIIGIGNDMIDIRRIEETLERFGDRFVNRIFTPVEQRKSDRRNK
                     RAASYARRFAAKEACSKALGTGLRKGVFWRDMGVVNLPSGRPTIALTGGAAAQLERIT
                     PAGHRAQIDVTITDDFPWAQAIVIISAVPSSGA"
     gene            5141..5968
                     /gene="lepB"
                     /locus_tag="PK264_14935"
     CDS             5141..5968
                     /gene="lepB"
                     /locus_tag="PK264_14935"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRD77201.1"
                     /translation="MVTRKRAPETWPEAIWDFARFIIYFALIWYVIRCFLFQMFNIPS
                     GSMKETLLVGDYIVVSKTSYGYSRYSFPRIATLCIPRTDKCVSFRVLPDLVTGRVWAA
                     TPKRGDVAVFRLPSNTSTDYIKRVIGLPGETIQVKGGIVYIDGKAVPRQRVDDFVERD
                     AEGRIRRTPRYEEVLPNGVKFHVLDEMQDGPFDDTALFKVPEGHYFMMGDNRDNSLDS
                     RDQTPDGVGFVPLENFIGRANAVVISLALEEPWRFPLREPWRWPSDVRWGRFFSGIR"
     gene            6128..6958
                     /gene="lepB"
                     /locus_tag="PK264_14940"
     CDS             6128..6958
                     /gene="lepB"
                     /locus_tag="PK264_14940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRD77202.1"
                     /translation="MSSLQSHSTRTQHGGWREVVSIVVYALVLAFLVRVFFFQPFNIP
                     SGSMKGTLLVGDYLFVSKFSYGYSKYTFPRIVTLCVPFTENCVTFRVLPDLFAGRVMA
                     ERPKRGDVAVFKLPRDNSTDYIKRVIGLPGDVILMKDGVLHINGVAVPKRRVEDFIEY
                     DEAGNARRTARYEETLPEGVKYFVLDQYPNNSSDNAGPFKVPADHYFMMGDNRDNSTD
                     SRDQSRGGVGFVPFENFVGRALVIFFSAATEEAWQFRLTRPWRWPADIRWERFFTRIK
                     "
     gene            6976..7686
                     /gene="rnc"
                     /locus_tag="PK264_14945"
     CDS             6976..7686
                     /gene="rnc"
                     /locus_tag="PK264_14945"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRD77203.1"
                     /translation="MTGARKYKDLEAALGYRFKSRELLSRALTHASIRASKTERMDNE
                     RLEFLGDRVLGLAIAELMIERHPQAPEGELARRFNKHVRREACAAVARKLDLGRLLIL
                     SDSEAGSGGRDKDTILADAMEALLAGLFLEAGFEGARAVIRELWAGIGEIGPSSAMTD
                     PKSALQEWAQGRGLALPEYVELHRTGPDHQPRFTAEVRIAGVKPATGEGTSKRSAEQA
                     AATHLLSREGVWSIETNG"
     gene            7679..8590
                     /gene="era"
                     /locus_tag="PK264_14950"
     CDS             7679..8590
                     /gene="era"
                     /locus_tag="PK264_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRD77204.1"
                     /translation="MADEAETRRCAFVAIIGAPNAGKSTLVNALVGTKVTIVSHKVQT
                     TRMPVRGIVMAGASQIVLVDTPGIFQPKRRLDRAMVGAAWGRAGDADIVLLLIDAARG
                     IDEDTGRILTGLGRLKRPAILALNKVDRVEKESLLALAKELNERHTFDETFMISALDG
                     SGVAHLKARLAERAPPGPWHFPEDDVSDLPMRMLASEITRERIYKWLHDELPYSITVE
                     TQAWKELRDGSVRIEQTIFVERDGQKGIVLGKGGHTVKQISSEARSEIASVIEKPVHL
                     FLFVKVRERWGDDPERYREMGLDYPKD"
     gene            8614..>9215
                     /locus_tag="PK264_14955"
     CDS             8614..>9215
                     /locus_tag="PK264_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRD77205.1"
                     /translation="MTANSGAGHAQSRPDAVAAMLRPRSIAIVGATPDPIKLNGRPQF
                     FLERDGYAGEIWPVNPRYADIRGRKCYPDIAALPGAPDMAIIAVAAERAREIVAALGA
                     RGCPVAVLFSSGFGELGEAGKRLEAELVATARATGIRLCGPNTLGLINAFERMPATFS
                     QYADAPPLAGPCGFASQSGAFGTGISAMARSRGLGLGYFVS"
     assembly_gap    9216..9326
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9327..>10384
                     /locus_tag="PK264_14960"
     CDS             <9327..>10384
                     /locus_tag="PK264_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRD77206.1"
                     /translation="EKALDAGKPFVLTKVGRKAAGMRAAASHTGSLAGEDRVFDAVVR
                     QAGILRARNEEHMLDVMAALVTNPVPSGRGIAIITQSGGAGVLMADRAEEIGLSVPVL
                     AEATQAALRKVLPEFGAAANPIDVTGAFLADPRILTESVRIALEDPAVDVGVIWLQLM
                     HGHADKLVELFRQIRREVAKPFLVCWLEAPERSRAALMADGIAVFAATERTIDAAKGL
                     VDWGASLRRHRSRRQTSRPGAARHHTAAGAGGDVATVPSMVARDLLEAAGLRTVTTRL
                     AADEASAVAAANELGYPVAIKVESTGIPHKTEADGVILGAKTPEDVARAVARVMAGAR
                     RHQPDATVDGVLVQRMAA"
BASE COUNT         1791 a         3283 c         3570 g         1629 t
ORIGIN      
        1 cggggatcgg cacggccgag agcagcgcct cggtgtaggg gtgcttcggg ttggagaaca
       61 gcgtgtcggt cggcgcctcc tcgacgatgc ggccgagata catgacggcg acccggctcg
      121 ccatgtgctc gacgacggcg aggttgtggc tgatgacgag gtaggtgagg ccgagcgacc
      181 ggcgcagctc ctcgagcagg ttgaggatct gcgactgcac cgagacgtcg agcgcggtcg
      241 tcggttcgtc gcagatcacc atgcgcggcc ggttgatgag ggcgcgggcg atcgcgacgc
      301 gctggcgctg gccgccggag agctggctcg ggtagttgtc gtaaaggcgg cggttgaggc
      361 ctacgagctc catcatctct tcgacgcgac ggcgccacgt cgcgggctcg ggatcgccct
      421 gaaccttcaa cggaagcgcg atgatcgagc cgatcgactt tctcggattg agggacgagt
      481 acgggtcctg gaacacgggc tgcacgaggc gcgccacctc atggcgcggc aggctgccga
      541 tcggcttgcc gtcgatctgg atctcgcccg aggtcggcgc gaggagcccc aagagcatgc
      601 gcgcgagcgt ggtcttgccg caacccgatt ctccgacgag cgcgacaacc tcgccctgcg
      661 caaccgagag cgagatgcca ttgacggcgc ggagcggtcg cttgccgcgc atgaagccgc
      721 gcgaaaccat gaatgtgcgc gagacatcgc gtgcttcgag cattgccgtc atgctgtggc
      781 tcctgccggc atcatcgcgc cgctcttcag gcatcggacg agatggacgg acgccgtcgt
      841 cgtggtcgtc cgcggcggcg cgagggccac gggggctgcg cggcagccgg cgtccccgat
      901 cgggcagcgg ctcgcgaaat ggcagccctg gatggcgccg acgagcgagg gcaccatgcc
      961 ggggatagtg ccgagcgcgt cacctcgctt cgtgcggcct gggatcggga tgcaggcgag
     1021 gagcccgcgc gtgtaggggt ggcttgggcg gcggaagatc tgctcggccg tgcccgtctc
     1081 gaccacctgg cccgcgtaca tgacgacgac gcgcgtcgcg atccgggcaa cgatcccgag
     1141 atcgtgggtg atgagaatga gccccatgcg cagctcccgc tgcaactcgg cgagcaggtg
     1201 gaggatctgc gcctggatcg tcacgtcgag tgcggtcgtc ggctcgtcgg cgacgatcag
     1261 gtccggtccg cacatcagcg ccatggcgat catgacgcgt tggcggagcc cgcccgagag
     1321 ctggtgggga tactggccga ggcgctcggc tgccgccgtg atgccgaccc gctcgagcag
     1381 gttgatcgcg cgggcgcgcg cgtcactcgt cgagacgcgc ttgtgccggc gcatcgcctc
     1441 aatgagctgg ttaccgatcg tgtaggccgg attgagcgac gtcatcggct cctggaaaat
     1501 catcgccatg cggttgccac gcaggtccga catctgccgc tcggataggc gcgtcatgtc
     1561 ggtgtcggcg atctccaggc ggcgggcgct gcgcttagcg cgcgagggga ggaggtccat
     1621 gatggcgagc gcggtcagcg acttgccgca gccggattcg ccgacaatgc agaccgtctc
     1681 gccgcgatcg accgtgaagg acacatcctg cacgggatgc agcgtgcccg cggggacagg
     1741 gatctttacg tcgaggccgt cgacggtgag gatgcgctcg gtcatgcggg ctctgctgct
     1801 tcgatgaagt cttgcggtgc cacgatatcg atcccgcgaa aggggtggag atttaggaga
     1861 tcgtcgtcgc gggtgaggat catgcccggt tctccggcgc cgtcacatcg cgcaggccgt
     1921 cgccgagcag gttgatcgac aagacgagca agaagagtgc gaggccaggg atcgcaatca
     1981 gccacgcttc aaagaacatg aggttcttgg cttccgaaac catcagtccc catgacggcg
     2041 tcggcggctg cacgccgaga cccaggaacg agagcgcggc ctcgagcagc accgcatgag
     2101 ccatctcgag ggtggcgacg acgatcaggt ggttcatcac gttgggcgcg acctcggtca
     2161 gaaggatgcg cgtcgtcgag cagccgaccg cctccgccgc cgtgatgaac tcgcgcgagc
     2221 gcacctgctg cgtcgccgag cgcatgacga cggcgaagcg gtcccagatg agaagtccga
     2281 gcacccagat gacgacctgc agcgacgagc cgacgaggct cacgaccgcg agtgcaacca
     2341 gcacgacggg catcgagaga cgtgtcgtga cgatgaaatt cacgaccatg tcgacccggc
     2401 cgccgaaata gccggcgata acgccgagcg tggtgccgat ggcgccagag atgagcatcg
     2461 cggcaaagcc gatgtagagc gagacgcggg cgccgtggat gaggcgcgag agatagtcgc
     2521 ggccgaggtg gtcggtgccg aggatatgcg cccacgtcgc cttggcgctc gcgtgccaga
     2581 tgggagggat gagcttgcgc tccaagtcct gagcgagcgg atcgtgcggt gcgatgacgt
     2641 cggcgagcag cgccatcagg atcatgacga tcagtacgag cgagccgatc atcagccccc
     2701 agtgacggac gatgcgccgg cgcatgagcg cgccgggcga aggcgccagg atttcggcgg
     2761 cggtcggcat gccggcggcg ggagcgccga tcgagacgtt ggccatgtca tgccacccgg
     2821 atgcgagggt cgaggaaggc gttcaggaga tcggcgagaa aggtcaggag gatgtagatc
     2881 atcgaaagga tgagcacgat cgcctgcatc atcggcaggt ccttccgctg gatcgattcc
     2941 caggcgagat agccgaggcc attgatctgg aaaatcgatt cgatcacgat cgagccgccg
     3001 agcatgaagc cgaattggac cgcggcaagc gacacgaccg ggatgatggc gttcctcaac
     3061 gcatgcttga acagcacggt gatcggccgg aggcccttgg cgcgcgccgt gcggatgtag
     3121 tccgaggcga gcacgtcgag catgcctgcg cgggtcagcc gcatcacgcc cggcgctaga
     3181 tagtagccaa gcgcgattgc cggcatcacg aagttcgccc agctgtctga gccggtgata
     3241 gggagctgga tgcgcgtcga ccaccagtag agattgatgt tgaggccata gatgccgagc
     3301 cagagcatca aggtgagcgc gaaccaaaac gagggcattg cctggccgag cacggcgatg
     3361 ccgagcgcgg tgcggtcgac gatgctgttc gggcggacgg ccgcgacgac gccgagcggg
     3421 atcgcgagga caagcgcgaa ggtgatcgcg cagagcgcca aggtcatcgt gacggggagg
     3481 cgcgagaaga tgacgtcggc gaccggctgg cggagattgt aggagcggcc gagatctccg
     3541 gtcacggcgc ccttgagcca gtcgaagtac tgcaccggga taggccggtc gaagccgtag
     3601 aacttgcgga tgttgtcgat gtcctcgggc cgcgcgccct cgcccgccat ggcgatcgcg
     3661 ggatcgccgg agacatagat catcgagaag gtgatcagcg acacggtgag cgcgaccagg
     3721 atcgcgatgc cgagacgctt caacgagtag acgagcatgg gacctcatcg ccggacgagc
     3781 gcccacgata gaggcgagcg cgccccgcca caacggtttc actgagccgt ggttcaccag
     3841 tcccgatatc agcacacgcg aatgttggtg ccgcatgcac ggaggcgaca cctgcgcgac
     3901 ggtgtgctat cggcaactgc tcccgctcaa cggatgtccg acatgcgccg aagcgacgac
     3961 agcaagcatc tgagacttgg cgtcaacatc gatcacgtgg cgaccgtgcg caatgcccgc
     4021 ggcggcgcgc atcccgaccc cgtacgcgcg gcgcaggcgg cgatcgaggc gggcgccgac
     4081 ggcatcaccg ctcacctgcg cgaggatcgc cgtcacatcc gcgacgacga tatccgccgg
     4141 ttgaaggccg agatcacgcg cccgctcaac ttcgagatgg cggcgaccga ggagatggcg
     4201 cgcatcgcgc tcgcgacgct gccgaatgcc tgctgtctcg tcccggagaa gcgcgaggag
     4261 cgcacgaccg aaggtggcct cgatgtcgtc ggccagggga acacgctaca aggatatatc
     4321 gcgcggctaa aggcaaaggg tatccgcgtc tcgctgttca tcgagcccga cctgagggcg
     4381 gtcgaggcgt cggcgcgatt gggcgctgac atcgtcgagt tgcataccgg cacgtattgc
     4441 gagaaggcgc tcgcccatga tgccgaccgc gtgcgtctcg agatcgagcg catcgcgcgc
     4501 gcggcgaccc acgccaaggc cgcgggcctc gaggtgcacg ccggtcatgg cctcacctat
     4561 ctcaccgtcg gcccgatcgc gcgcatgccc gagatggtcg aactcaacat cggccacttt
     4621 ctcatcggcg aggcgatatt cgtcgggctc cgcggtgcca ttggcgagat gcgacgcctg
     4681 atggacgagg cgcgggcccg atgatcatcg gcatcggcaa cgacatgatc gacatccggc
     4741 gcatcgagga gacgctcgag cggttcggcg accgcttcgt caatcgcatc ttcacgccgg
     4801 tcgagcagcg caagtcggac cggcgcaaca agcgggcggc ctcctacgcc cgccgctttg
     4861 ccgccaagga agcgtgctcg aaggcgctcg gcaccgggct ccgaaaaggc gttttctggc
     4921 gcgatatggg cgtcgtcaat ctgccgtcgg ggcggccaac aatagccctc acgggagggg
     4981 ctgcagccca gctcgagcgg atcacgcccg cgggccacag ggcgcagatc gacgtgacga
     5041 tcaccgacga ttttccctgg gcgcaggcga tcgtcatcat ctcggccgtg ccgagttcgg
     5101 gcgcctgacg gctgagctca atcgagggga caagtctcgc atggtgactc gcaagcgcgc
     5161 accggaaacc tggccggaag ccatctggga tttcgcccgc ttcatcatct atttcgcgct
     5221 catctggtac gtgatccgct gcttcctgtt ccagatgttc aacatcccct cgggctcgat
     5281 gaaggagacg ctgctcgtcg gcgactacat cgtcgtctcg aagacgtcgt acggctacag
     5341 ccggtattcc tttccgcgca tcgccacgct ctgcatcccg aggaccgaca agtgcgtgtc
     5401 gttccgcgtg ctcccggacc tcgtgacggg acgggtgtgg gcggcgaccc cgaagcgtgg
     5461 cgatgtcgcc gtcttccgcc tgccgagcaa tacctcgacg gactacatca agcgcgtgat
     5521 cggcttgccc ggtgagacaa tccaggtgaa gggcggcatc gtctacatcg acggcaaggc
     5581 cgtgccgcgc cagcgagtcg acgatttcgt cgagcgggac gcggaggggc gcatccggcg
     5641 cacgccgcgc tacgaggaag tgctgccgaa cggcgtcaag ttccacgtcc tcgacgagat
     5701 gcaggacggt cccttcgacg ataccgcgct cttcaaggtg cccgaggggc actatttcat
     5761 gatgggcgac aatcgcgaca actcgctcga cagccgcgac cagacgccgg acggcgtcgg
     5821 cttcgtgccg ctcgagaact tcatcggccg tgccaatgca gtcgtgatct cgcttgcgct
     5881 cgaggagccg tggcggttcc cgttgcgcga gccatggcgc tggccctcgg acgtgcgctg
     5941 ggggcgcttc ttttccggga tcagatagtg tcaggccgtt gccgtttgcc cgattcgcgt
     6001 ggcgaaaccg caacatgaca ggattgcccg cgtcagtcgt gcaattgccc gggatcgccc
     6061 ttcgagctat aggagcgcct tgcgacccct ttcgcgcgcc gtagcggatg ggccgatcgg
     6121 aggcctgatg agcagcctgc aatcccattc gacgcgcacg caacatggcg gctggcgtga
     6181 ggtcgtcagc atcgtcgtct atgcgctcgt tctcgcgttc ctcgtgcgcg tgttcttctt
     6241 ccagcccttc aacattccgt cgggctccat gaagggcacg ctgctcgtcg gcgactacct
     6301 gttcgtctcg aaattctcct acggctacag caagtacacg ttcccgcgca tcgtgacgct
     6361 gtgcgtcccc ttcaccgaga actgcgtcac cttccgcgtg ctccctgatc tcttcgccgg
     6421 gcgcgtgatg gcggaacggc cgaagcgcgg cgatgtcgcg gtcttcaagc tgccacgcga
     6481 caattcgacc gactacataa agcgggtgat cggcctgccg ggagatgtca tcctgatgaa
     6541 ggacggcgtc ctgcacatca atggcgtcgc cgtgccgaag cggcgcgtcg aggacttcat
     6601 cgagtacgac gaggctggta atgcacggcg cactgcgcgc tacgaggaga ccttgccaga
     6661 gggcgtcaag tatttcgtcc tcgaccagta tcccaacaac tcctcggaca atgccggccc
     6721 cttcaaagtg ccggcggacc attacttcat gatgggggac aacagggaca attcgacgga
     6781 cagccgcgat cagagtcgcg gcggcgtggg cttcgttccg ttcgagaatt ttgtcggccg
     6841 cgccctcgtg atcttctttt ctgcggcgac cgaggaggcc tggcagttcc ggctgacgcg
     6901 gccctggcgc tggcccgccg atatccggtg ggagcgcttc ttcacgcgga tcaagtgact
     6961 cctcggagcg agcgtgtgac cggagcgcgc aaatacaagg acctcgaggc ggccctcgga
     7021 taccggttca agagccgcga gctgctgtcg cgggcgctca ctcatgcgag catccgcgcg
     7081 tccaagacgg agcgcatgga caacgagcgg ctcgagtttc tcggtgaccg cgtgctcggt
     7141 ctcgccatcg ccgagctaat gatcgagcgg catcctcaag cccctgaggg cgagttggcg
     7201 cgccgcttca acaagcatgt gcggcgcgag gcctgcgccg ctgtcgcgag gaagctcgac
     7261 ctcggccggc tgctgatcct gtccgactcc gaggccggca gcggcggacg cgacaaggat
     7321 accatcctgg ccgacgccat ggaggcgctg ctggccggac ttttcctcga ggcaggcttc
     7381 gagggggcgc gagccgtcat ccgtgagctg tgggcaggga tcggcgagat cgggccatcg
     7441 tccgcgatga cggatccgaa gtcggcgctg caggagtggg cgcaggggcg cgggctggcg
     7501 ttgccggagt acgtcgagct tcatcgcacc gggcctgacc accagccgcg ctttacggcc
     7561 gaggtgcgca ttgccggcgt caagcccgca acgggcgagg ggacgagcaa acggtcggcc
     7621 gagcaggccg ctgcaaccca cctcctctcc cgggaaggtg tttggagcat cgaaaccaat
     7681 ggctgacgag gccgagacgc ggcgttgcgc gttcgttgcc atcataggag cacccaacgc
     7741 cggcaagtcg acgctcgtca acgcgctcgt cggcacgaag gtgaccatcg tcagccacaa
     7801 ggtgcagacg acgcgcatgc cggtccgcgg catcgtcatg gcgggagcga gccagatcgt
     7861 tctcgtcgac acgccgggca tcttccagcc gaagcgccgg ctagaccgcg ccatggtggg
     7921 cgccgcctgg ggccgggccg gcgatgccga catagtcctg ctgctgattg atgcggcgcg
     7981 cggcatcgac gaggacaccg ggcgcatctt gaccggcctc ggccgactga agcgtccggc
     8041 gatactcgcc ctcaacaagg tcgaccgggt cgagaaggag agcctgctcg cgctcgccaa
     8101 ggagctcaac gagcgccaca cctttgacga gacattcatg atctcggcgc tcgacggcag
     8161 cggcgtcgca cacctgaagg cgcggctcgc cgagcgcgcg ccgccggggc cgtggcactt
     8221 ccccgaggat gacgtctccg acttgccgat gcgcatgctc gcctccgaga tcacgcgcga
     8281 gcgcatctac aaatggctgc acgacgagct gccgtactcg atcacggtcg agacgcaggc
     8341 ttggaaggag ctgcgagacg gctctgtccg tatcgagcag actatctttg tcgagcgtga
     8401 cgggcagaag gggatcgtgc tcggcaaggg cgggcatacg gtgaaacaga tctcgagcga
     8461 ggcacgctcg gagattgcct cagtcataga gaagcccgta catctcttcc tgttcgtgaa
     8521 ggtgcgcgag cgctggggcg acgaccccga gcgctaccgc gagatggggc tcgactaccc
     8581 caaggactga cgcgccagga tgcgagggat gaaatgaccg ccaacagcgg ggccgggcat
     8641 gcgcagagca ggcccgatgc agtcgcagcc atgctgcggc cgcgctcgat cgccatcgtc
     8701 ggcgcgacgc ccgacccgat caagctcaac ggccggccgc agttctttct ggagcgcgac
     8761 ggctatgcgg gcgagatctg gccggtgaac ccacgctatg ccgacatccg tgggcgcaag
     8821 tgctatccgg acatcgccgc tctccccggc gcgcccgaca tggcgatcat cgcggttgcg
     8881 gctgagcgcg cgagagagat cgtggcggcg cttggcgcca ggggatgtcc ggtcgcggta
     8941 ctcttctcgt ccgggtttgg cgagctcggc gaggcgggga agcgtctcga ggcggagctc
     9001 gttgcaacgg cgcgggcgac cggcatccgc ctctgcggcc ccaacacgct cggcctcatc
     9061 aatgccttcg agcgcatgcc cgcgaccttc agccagtatg ccgatgcccc gccgctcgct
     9121 ggcccctgcg gcttcgcgag ccaatcgggc gccttcggca ccggtatctc cgccatggcg
     9181 cggagccgcg gcctcggcct cggctatttc gtctcnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnccga aaaggcgctc gatgcgggaa agccgttcgt
     9361 cctcaccaag gttgggcgca aggcggccgg catgcgggcc gccgcgtcgc acacgggctc
     9421 gctcgctggt gaggatcgcg tgttcgatgc ggtcgtgcgg caggcgggca tcctcagggc
     9481 gcgcaacgag gagcacatgc tcgacgtcat ggcggcgctc gtcacgaacc cggtcccgag
     9541 cggccgcggc atcgccatca tcacgcagtc gggtggtgcc ggcgtgctga tggccgatcg
     9601 cgccgaggag atcggattgt cggtgcccgt gctggccgag gcgacgcagg cggcgctgcg
     9661 caaggtgctt cccgagttcg gcgcggcggc gaacccgatc gacgtcaccg gcgcgtttct
     9721 cgccgatccc cgtatcctga ccgagagcgt gcgcatcgcg ctcgaggatc cggccgtgga
     9781 cgtcggcgtg atctggctgc agctcatgca tgggcatgcc gacaagctcg tcgagctctt
     9841 ccgccagatc cgccgtgagg tggcgaagcc cttcctcgtc tgctggctcg aggcgccgga
     9901 gcgctcgcgg gcagccctga tggccgacgg catcgcggtc ttcgcagcga ccgagcgcac
     9961 gatcgatgcc gcaaaggggc tcgtcgactg gggggcttcg ctgaggcgac accggtcgcg
    10021 acggcagacg tcacgcccgg gagcagctcg gcaccacacg gccgccggag caggcgggga
    10081 tgtcgccacc gtcccgtcga tggttgcgcg cgatctcctc gaggccgcag gcctcaggac
    10141 cgtcacgacg cgcctcgcgg ccgacgaggc aagcgcggtc gccgcagcga acgagctcgg
    10201 ctatcccgtc gcgatcaagg tcgagtcgac cggcatcccc cacaagaccg aggccgacgg
    10261 cgtcatcctc ggcgccaaga cgccggagga cgtcgcgcga gccgtcgcgc gggtgatggc
    10321 cggcgcgcgg cgccatcagc ccgatgcgac cgtcgacggc gtgctcgtcc agcgcatggc
    10381 cgcg
//
LOCUS       DAORNH010000151        10381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4777_length_10381_cov_2.539221, whole genome shotgun sequence.
ACCESSION   DAORNH010000151 DAORNH010000000
VERSION     DAORNH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4777_length_10381_cov_2.539221"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK264_14965"
     CDS             <1..506
                     /locus_tag="PK264_14965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD77207.1"
                     /translation="ITAMPLEAAAALPRLSTFRREELRSLVAPLRQIGLRRGETAIGA
                     GRPAARCLLVLRGALALHFAHEGQQSVFAIIGPGGIAGEIALLDGAPEPLTALAREDT
                     LALEIDKLTFDILARGGTTVAHKFLAAVTTSATAVLRRASAHLARLEGAVQVADTITP
                     IERREAS"
     gene            507..1436
                     /locus_tag="PK264_14970"
     CDS             507..1436
                     /locus_tag="PK264_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HRD77208.1"
                     /translation="MCRVLIYKGEPVSLDALLFRPNNSLIRQSYEPQLLDALSLAGFG
                     MLAWDDRSPRPAMPFAYKTPGIPLFDDTLKSLAEKIDVTTLIAHVRGVAYEAAPTIGE
                     QNLHPFRYRGMSLALAHNGQLAGFERMRFALLAHIKPEIARLIRGNTDSEWIYALLLS
                     ALDDPTATQSSDAILAALERTLVVLRTVRHEHNIAISSPANLFVTDGTSIVATRFTFD
                     FGCYDPSDVGAIAAASHRYLDMWYTTGGTYAFEEGEWRMSSVPAGETRSLIISSEPLT
                     RDPSTWFEVPEYSALVVSIVSGQMSHRVKFLDA"
     gene            1463..2428
                     /locus_tag="PK264_14975"
     CDS             1463..2428
                     /locus_tag="PK264_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HRD77209.1"
                     /translation="MSHGTPLDVLTLSAGDVARLIDPKAAIDALAKGFEALSRGEVQV
                     PPRPMVESAGKGFAIAMLASAPGQNIALKLVSVFGDNARLGLPSHHAVINLLSPETGQ
                     PVATMDGGVITAVRTAAASMVTVRALARPEARIATVIGGGVQGRSHVELLSHVRDFAE
                     IRVASRRRESAEAIAAGIARTVVTTDIEGAVRASDVICLCSSSDRPVIEASWVKSGTH
                     ITSVGFVAKGGELPPALIDRARLFVESKAAFSPPPVGASELAGLDPARGAEIGEMLLG
                     LKCGRQSADEITIYKSMGNAMEDLVLANLVYAAAKERGIGRIVAL"
     gene            2425..3345
                     /locus_tag="PK264_14980"
     CDS             2425..3345
                     /locus_tag="PK264_14980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD77210.1"
                     /translation="MNTRQRNKRMPKTLAFIGLGAMGLPMAERLVGAQLRVVGFDLRP
                     EAMVTLEAKGGHGARSIAEAARGADTLITMVVNADQAEDVLFAKGAVEALAPGASVIV
                     MATCAPARVQAMAARVKATGRAFIDAPVSGGVAGASTGTLTIMAAAPEADFARVKPVL
                     EVLGNKLFHLGEAAGQGAAMKVVNQLLCGVHIAVAAEGLAFAESAGIDPAKALEILSG
                     SAAQSWMLNARGKRMVADDGQVTSAVDIFVKDLSLVLDAGRAMKAGLPLAAAAHQLFL
                     AASGMGHGSRDDSQVIETYRALAPKGAKRS"
     gene            3386..>4067
                     /locus_tag="PK264_14985"
     CDS             3386..>4067
                     /locus_tag="PK264_14985"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HRD77211.1"
                     /translation="MAKASAAQIEAFERDGVVLLPGLLADRVTALEAAVAENMASPFE
                     RTYKPDDGGAPFFQDFCNWARIPGYRSAVLESPMAEVAAALMRSGTARFFHDHVLVKE
                     PGNSMKTPWHQDQPYYLVEGEQSVSFWVPLDPVPREIALEYVGGSHRAGRMFRPERFD
                     GTSLYQGDDSERVPDVDGRRAEFNILAWPMQPGDAVAFNFRTLHGAPANTTKNRRRAI
                     SFRWVGDDA"
     assembly_gap    4068..4101
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4195..5712)
                     /gene="lnt"
                     /locus_tag="PK264_14990"
     CDS             complement(4195..5712)
                     /gene="lnt"
                     /locus_tag="PK264_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRD77212.1"
                     /translation="MAAMAPLFAWPVLLFTLPALTWLLDGDVNRANGNGWARLRAAFA
                     TGWWFGFGYFFGGLFWIGEAFLVEAEMFGWALPFAVTLMPAGLGVFYGLAATAATLAW
                     RPGLTRLVALALAIAIAEWLRGHVLSGFPWNTLGYALTAPLMLAQSASVLGIHGLTLI
                     VMLVLPAPLILLEDARAGRIGQSWRFAGILAALVMLTAMTSFGMLRLAAGLAPMLEGV
                     RLRLVQPSIAQREKWRPEHQERIFFEHLALSGASPAGLAGITAVIWPEAAMPFRPLDS
                     PEALDHVGRLLPIGVHLIAGGLRMDEEPATQSRRVYNSLLVFGVGGAPVAIYDKLHLV
                     PFGEYLPLQPLLEAIGLRQLTKLRGGFARGAAPRALLRIPGLPTIGPLICYEAIFPGS
                     LIQTPERPGLLVNVTNDGWFGETTGPHQHLHQTRVRAIEEGIPIARVANNGISAMIDP
                     YGRILQRLELNVKGVIDTGVPRAITPPLYARTGDFVFLGMLAGLTVVLVVLLRRT"
     gene            complement(5814..6737)
                     /locus_tag="PK264_14995"
     CDS             complement(5814..6737)
                     /locus_tag="PK264_14995"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRD77213.1"
                     /translation="MDARVKTRPAEGHAPVSQIRTVGIVGAGQMGNGISHVIALAGYD
                     VKLYDLKKEAIEKAIETIKRNMTRQVSRGLIKDGDMQAALGRIAPVTSVSGLANVDLV
                     IEAATEDEAIKRKIFAELCPVVKKGALLASNTSSISITRLAASTDRPERFIGMHFMNP
                     VPLMQLVELIRGIATEDDTFGVARSFVESLGKTIAVSEDFPAFIVNRILLPMINEAVY
                     TLYEGVGTVEAIDKAMRLGANHPMGPLELADFIGLDTCLSVMQVLYEGLADSKYRPCP
                     LLVKYVEAGWLGRKTQRGFYDYRGEKPVPTR"
     gene            complement(6807..7751)
                     /locus_tag="PK264_15000"
     CDS             complement(6807..7751)
                     /locus_tag="PK264_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD77214.1"
                     /translation="MAVLLVADHNNAAVGAQTLKAMSAAKALGGDVHVLVAGAGCNPA
                     AEAAAKIDGAAKVLLVDAPSLANGLAEEMAALIVPMMANYSAVVAPATASGKNVLPRV
                     AALLDVMQISDIVAVKSADTFDRPIYAGNAIQTVQSSDTKKVITVRTAAFPAVGTSGA
                     APIEAVAAPAAVGLSTFEKSELSKSDRPELASAKIVVSGGRGLQSGDNFKKYIEPVAD
                     RLGAAMGASRAAVDAGFVPNDYQVGQTGKVVAPELYVAVGISGAIQHLAGMKDSKVIV
                     AINKDGEAPIFQVADYGWVTDLFQALPELETELKKVGR"
     gene            complement(7751..8500)
                     /locus_tag="PK264_15005"
     CDS             complement(7751..8500)
                     /locus_tag="PK264_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRD77215.1"
                     /translation="MKILVPVKRVVDYNVKIRVKADGSGVELANVKMSMNPFDEIGVE
                     EAIRLKEKGVAKEIIAVSIGSAKTQETIRTALAMGADRGIQVETGDDGIEPLTVAKLL
                     KALVDAEKPDIVILGKQAIDDDCNQTGQMLAALLGWSQGTFASKIAVEGGALAVTREV
                     DGGLETLKLKLPAIITTDLRLNEPRYASLPNIMKAKKKPLDVKKPADLGVEVKARLKV
                     LKTTEPAGRKAGIKVASGAELVAKLKDRGII"
     gene            complement(8787..>9061)
                     /locus_tag="PK264_15010"
     CDS             complement(8787..>9061)
                     /locus_tag="PK264_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRD77216.1"
                     /translation="GRPMIVHNRGWGPQLEDALFADQITGHYRYTGVLPPAGGPRSRK
                     LGEPTLVRRPLCAGGRPEVSIGPNVICPASAAATGRSAALPGPRAR"
     assembly_gap    9062..9223
                     /estimated_length=162
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9224..>10082)
                     /locus_tag="PK264_15015"
     CDS             complement(<9224..>10082)
                     /locus_tag="PK264_15015"
                     /inference="COORDINATES: protein motif:HMM:NF018626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRD77217.1"
                     /translation="MGAETTIASIDIGRLPDVLRGEVPVAAIDVGLLPQPAPAEVAIG
                     GLDLGQLPPPLGEEVATATVDVGALPPPLPPEIATAALDASGFPTPDTPASADRRAQP
                     HDLILRHLPGFLMGPRPKHWPNHLPPLAKAPLPSAETPPVDLAAAAPIDAPPVPTVRP
                     PTSDAPRVDAPRVAVPADATLERCEPSPLVLSHLTGMKSGHARPAPSDGATFGMALAA
                     AAVAETREFGIYDARYVRISYPLGDVPRLYGACTDLVIRAYRGVGVDLQRLVHESRAG
                     SGDRSIDHRR"
     assembly_gap    10083..10092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10093..>10381)
                     /locus_tag="PK264_15020"
     CDS             complement(<10093..>10381)
                     /locus_tag="PK264_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77218.1"
                     /translation="SLGDRSRTGQPGGAASSPELGRGRERRLASLVALPAVIVTAFLA
                     LQPGPALEGARAAAVLDPERPTLSVAPPAIVPAPALGPETATASLEVNALPP"
BASE COUNT         1690 a         3411 c         3439 g         1635 t
ORIGIN      
        1 ccatcactgc gatgccgctc gaggccgccg ctgcgctgcc acgcctcagc acattccggc
       61 gcgaggaact ccgctcgctc gtcgccccgc ttcgccagat cggtctgcgg cgcggagaga
      121 cagcgatcgg ggcggggcgg cccgcggccc gctgcctcct cgtcctgcgc ggcgcgctcg
      181 cactacattt cgcgcacgaa gggcagcaat ccgtattcgc gatcatcggt ccgggcggca
      241 ttgccggcga gattgccctc ctggatggcg ctcctgagcc actcaccgcg ctcgcacgcg
      301 aggacacgct ggctctcgag atcgacaagc tgaccttcga catcctcgct cgcggcggca
      361 cgaccgtcgc acacaaattt ctggcggccg tgacaacaag cgcgacagcc gtattgcgcc
      421 gcgcgagcgc gcatctcgcc cggctcgaag gcgcggtgca ggtcgccgac accatcacac
      481 ccatcgaacg tcgcgaggcg agctgaatgt gccgtgtgct catctacaag ggagagccgg
      541 tcagcctcga tgccctgctt ttccgcccca acaattcgct gatccggcag agctacgagc
      601 cacagctcct cgacgcactg agcctggccg gcttcggcat gctggcctgg gacgatcgct
      661 ccccccggcc cgcgatgccc ttcgcctaca agacccccgg cattccgctc ttcgacgata
      721 cgctgaaatc gctcgctgaa aagatcgacg tgacgacgct gatcgcgcac gtgcgcggcg
      781 tcgcctatga ggctgcaccg accatcggcg agcagaatct acacccgttt cgctatcgcg
      841 gcatgagcct cgcgcttgcg cacaacggtc agctcgccgg gttcgagcgc atgcggtttg
      901 cgttgctcgc ccacatcaag ccggaaatcg cgcggctcat tcgcggcaac acggacagcg
      961 agtggatcta tgcgctgttg ctctcggcgc tcgacgatcc gaccgccacc caatcaagcg
     1021 atgcaatcct cgcggcactc gagcggacgc ttgtggtcct gcgtactgtc cgtcacgagc
     1081 acaacatcgc gatttcctcg cccgccaatc tcttcgtgac cgatggcacg agcatcgtcg
     1141 ccacccgctt cacgttcgat ttcggctgct atgatccgag cgacgtcggc gcgatcgccg
     1201 ccgcgagcca ccgctatctc gacatgtggt acacgacggg tggaacatat gccttcgagg
     1261 agggcgagtg gagaatgtcg tcggtgccgg ccggcgagac ccgctcgctc atcatctcgt
     1321 cggagccgct cacgcgcgac ccctcgacct ggttcgaggt gcccgagtac agcgcactcg
     1381 tggtatcgat cgtgagcgga cagatgagcc atcgcgtgaa gttcctcgac gcctgaggcg
     1441 cgagacgagc aggagtgaac ccatgtcgca cggaacgccc ctcgatgttc tcacgctgtc
     1501 ggcgggcgac gttgctcgcc tcattgaccc gaaggccgcg atcgatgcgc ttgccaaggg
     1561 cttcgaggcg ctgtcacgcg gcgaggtgca ggtgccccca cggccgatgg tcgaaagcgc
     1621 ggggaagggc ttcgcgattg ccatgctggc ttcggcgccg ggccagaaca tcgcgctgaa
     1681 actcgtcagc gtgttcggcg acaatgcccg cctcggcctg ccgtcgcatc atgccgtgat
     1741 caatcttctc tcgcccgaga cgggccagcc cgtcgccacc atggacggcg gcgtgatcac
     1801 ggcggtgcgc acggcggcgg catcgatggt gacggtgcgg gcactggctc gccccgaggc
     1861 ccgcatcgcg accgtgatcg gcggcggcgt gcagggccgc agccacgtcg agctcctgag
     1921 ccacgtccgc gacttcgcgg agatccgcgt cgcctcgcga cgccgggaga gcgccgaggc
     1981 gatcgccgcc ggcatcgccc ggacggtcgt caccaccgac atcgagggcg ccgttcgcgc
     2041 gtccgacgtc atctgcctct gctcctcgtc tgaccggccc gtgatcgagg catcctgggt
     2101 taaatccggt acgcacatca cctccgtggg cttcgtcgcg aagggcggcg aactcccgcc
     2161 cgcgctgatc gatcgggcgc ggctgttcgt tgaatcgaag gccgccttca gtccgccgcc
     2221 ggtcggcgcc tccgagctcg caggcctcga ccccgcgcgg ggcgccgaaa tcggcgagat
     2281 gctgctgggc ctgaaatgcg gccgccaatc agccgatgag atcaccatct acaaatccat
     2341 gggcaacgcc atggaggacc tcgtgctcgc caatctcgtc tatgccgccg ccaaggagcg
     2401 cggcattggc cggatcgtcg ctctctgaac acccgccagc ggaacaagcg catgccgaaa
     2461 acactcgcgt tcatcggact gggcgccatg gggctcccca tggcggagcg gctcgtcggg
     2521 gcgcagctgc gcgtcgtcgg cttcgatctc cggcccgagg ccatggtgac gctcgaggca
     2581 aagggcggcc acggcgcacg ctcgatcgcg gaggccgcac gtggcgcgga cacactgatc
     2641 accatggtcg tcaacgccga ccaggccgag gatgtgctgt ttgccaaggg ggccgtcgag
     2701 gcactggccc cgggcgcaag cgtcatcgtg atggcgacct gcgcgcctgc gcgtgtccag
     2761 gcgatggcgg cgcgggtcaa ggccaccggc cgcgccttca tcgacgcgcc ggtgtcgggc
     2821 ggcgtcgcgg gcgcttcgac cggaacgctg acgatcatgg ccgcggcacc agaggccgat
     2881 ttcgcgcgcg tgaagcctgt gctcgaggtg ctcggcaaca agctctttca tctcggcgag
     2941 gccgcaggcc agggagcggc gatgaaggtc gtcaaccagc ttctctgcgg ggtgcatatt
     3001 gccgttgccg ccgaggggct ggcatttgcg gagagcgccg gcatcgatcc ggccaaggca
     3061 ctcgagatcc tgtcgggaag tgcggcgcag agctggatgc tgaatgcgcg cggcaagcgc
     3121 atggtggctg acgacggcca ggtgacgagc gccgtcgaca ttttcgtgaa ggatctgtca
     3181 ctcgtgctcg acgcagggcg cgccatgaag gcgggcctgc cgctcgccgc agcagcgcac
     3241 cagctgttcc tcgccgcctc cggcatgggt cacggcagcc gggacgacag ccaggtcatc
     3301 gagacatatc gcgcgctcgc gcccaagggc gcaaagcggt cctgatcggc ggagcgcaga
     3361 cgactcagaa cgcaaggagg tcaacatggc taaggccagc gcagcgcaga ttgaggcttt
     3421 cgagagggac ggtgtggtgc tgctgccggg actgcttgcg gatcgcgtga ccgcgctcga
     3481 ggcagccgtc gccgagaata tggcgagccc gttcgagcgg acctacaagc ccgatgacgg
     3541 cggggcgccg ttcttccagg atttctgcaa ctgggcgcgc atcccgggct atcgctcggc
     3601 cgtactcgag agcccgatgg cggaggtcgc ggcagccctc atgcgatcag ggaccgcgcg
     3661 cttcttccac gatcacgtgc tcgtcaagga gcccggcaac agcatgaaga caccgtggca
     3721 tcaggaccag ccctactatc tcgtcgaggg tgagcagtcg gtgagtttct gggtgccgct
     3781 cgatcccgtg ccccgcgaga tcgcgctgga gtatgtcggc ggctcgcatc gcgcggggcg
     3841 catgttccgg cccgagcgct tcgacgggac gagcctctat caaggcgatg acagcgagcg
     3901 ggtgccggat gtggacgggc gccgcgccga gttcaatatc ctcgcctggc cgatgcagcc
     3961 gggcgacgcc gtcgcgttca attttcgcac tttgcacggc gcccccgcca acacgacgaa
     4021 gaaccgccgc cgcgccattt cgttccgctg ggtcggcgac gacgcgcnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nccgccgttc ccgcacatcg cggtggtgga tggcgcgccg
     4141 ttcgagggac ccgatttccc actcgtctgg ccgaaaagga cctgacgacg ttgttcaggt
     4201 ccgccgcagg agcacgacaa gcacgaccgt gagccccgcc aacatgccaa gaaagacgaa
     4261 gtcacccgtg cgggcgtaga gcggcggcgt gatggcgcgg ggcacgcccg tatcgatgac
     4321 gcctttcacg ttgagctcga gccgctgcag gatgcggccg taggggtcga tcatggccga
     4381 gataccgttg ttcgccacgc gcgcgatcgg gatgccttcc tcgatcgccc tgacacgcgt
     4441 ctgatggaga tgctggtgcg ggcccgtcgt ctcgccgaac cagccgtcgt tggtgacgtt
     4501 gacgaggagc ccgggccgtt ccggcgtctg aatcaacgag cccggaaaga tcgcctcgta
     4561 gcagatcagg gggccgatcg tcggcagacc cgggatgcgg agcagcgcgc gcggtgcggc
     4621 acctcgcgca aagccgccgc gcagcttcgt caactggcgg agcccgatcg cctcgaggag
     4681 cggctgcagc ggcagatact cgccgaacgg cacgaggtgc agcttgtcgt agatcgcgac
     4741 cggcgcgccg ccgacgccga agacgagcaa tgaattgtag acgcggcgcg actgtgtggc
     4801 cggctcctcg tccatcctga gcccgcccgc gatcaagtgc accccgatcg gcaggagccg
     4861 gccgacatgg tcgagcgcct cggggctgtc gagggggcgg aacggcatgg cggcctcggg
     4921 ccagatcacg gccgtgatgc ccgcgaggcc ggcgggcgag gcgccggaga gcgccagatg
     4981 ctcgaagaag atgcgctcct ggtgctccgg ccgccatttc tcgcgctgcg caatgctcgg
     5041 ctggacgagc ctcaggcgca cgccctcgag catcggcgca agccctgccg ccaatcgcaa
     5101 catcccgaac gacgtcatgg cggtcagcat gacgagagca gcgaggatgc cggcgaagcg
     5161 ccagctctgc ccgattcggc ccgcgcgggc atcctccagc agaatgagcg gcgccggcag
     5221 caccagcatc acgatgagcg tcaggccatg gatgccaagc acactcgccg actgggcaag
     5281 catcagcggc gcggtgagcg cgtatccgag cgtgttccac gggaaaccac tgagaacatg
     5341 ccccctcaac cactctgcga tcgcgattgc cagggcgagc gcgacgagtc gcgtcaggcc
     5401 cgggcgccac gcaagtgtcg ctgcagtcgc ggccaacccg tagaaaacgc caagaccagc
     5461 cggcatcagc gtgacagcga acggcagcgc ccaaccgaac atctcggctt cgaccaggaa
     5521 ggcctcgccg atccagaaga ggccgccgaa aaagtagccg aagccgaacc accaacccgt
     5581 ggcgaaggcg gcacgcagcc gcgcccaacc gttcccgttg gcgcggttga catcgccgtc
     5641 gagcagccaa gtcaacgccg gaagcgtaaa gagcagtacg ggccaagcga agagcggcgc
     5701 catcgctgcc acgctgacga gtcctacggc tgtcgccaca attcgatgcc gccagccgac
     5761 cgcgccggcg aagaacgcaa ctgcagccgg acccgcgcgc tcgctcacga ggctcagcgc
     5821 gtcggaacgg gcttctcacc gcggtagtcg tagaagccgc gctgcgtctt gcggccgagc
     5881 cagccggcct cgacatactt tacgagtagc gggcagggac ggtacttgct gtcggcgagg
     5941 ccctcgtaaa gcacctgcat gaccgagagg caggtatcga ggccaatgaa gtcggcgagc
     6001 tcgagcgggc ccatcgggtg attggcgccg aggcgcatgg ccttgtcgat cgcctccacg
     6061 gtgccgacgc cctcatagag cgtgtagacg gcctcgttga tcatcggcag caggatgcgg
     6121 ttgacgatga aggcggggaa gtcctcggaa acagcgatcg tcttgccgag actctcgacg
     6181 aacgagcgcg cgacgccgaa cgtgtcgtcc tcggtcgcga taccgcgaat gagctcgacg
     6241 agctgcatca gcggcaccgg attcatgaaa tgcatgccga tgaagcgttc gggccggtcg
     6301 gtgctggcgg cgaggcgcgt gatcgagatc gacgacgtgt tggacgcgag cagcgcgccc
     6361 ttcttcacga ccggacacag ctcggcgaag atcttgcgct tgatcgcctc gtcctcggtc
     6421 gccgcctcaa tgacgagatc gacgttggcg agacccgaga cggaggtgac gggtgcgatg
     6481 cggccgaggg ccgcctgcat gtcgccatcc ttgatgaggc cgcgcgagac ctggcgcgtc
     6541 atgttgcgct tgatggtctc gatcgccttc tcgatcgctt ccttcttgag gtcgtagagc
     6601 ttcacgtcat agccggcgag cgcgatcacg tggctgatgc cgttgcccat ctggcctgca
     6661 ccgacgatgc cgacggtgcg gatctgggac acgggagcgt gcccctcggc aggtctcgtc
     6721 ttgacgcgag cgtccatcgt caggctcctg atgtgttgca ggcggcagtg ccgatccgga
     6781 tcggacgggg cgctgccgcg gaagtgtcag cgaccaactt ttttcagctc ggtctcgagt
     6841 tcggggagtg cctggaacag atcggtgacc cacccgtagt cggcaacctg gaagatcggc
     6901 gcctcgccat ccttgttgat ggcgacgatc accttgctgt ccttcattcc ggcgagatgc
     6961 tggatcgcgc cggagatgcc gacggcaacg tagagctcgg gcgcgacaac cttgccggtc
     7021 tggccgacct ggtagtcgtt cggcacgaag ccggcatcga ccgcggcgcg cgaagcaccc
     7081 atggccgcgc cgaggcgatc ggcgaccggc tcgatgtact tcttgaagtt gtcgccggac
     7141 tggagcccgc gtccaccgga gacgacgatc tttgccgagg cgagctcggg gcggtccgac
     7201 ttcgaaagct cggatttctc gaaggttgag aggccgacgg ctgcaggtgc cgcaaccgcc
     7261 tcgatgggcg ccgcgccgct cgtcccgacc gcggggaacg ccgccgtgcg caccgtgatg
     7321 actttcttgg tgtcgctcga ttggacggtc tggatggcgt tgcccgcgta gatcggccgg
     7381 tcgaaagtgt cggcagactt caccgcgacg atgtcggaga tctgcatgac gtcgaggaga
     7441 gcggcaacgc gcggcagcac attcttgccc gacgcggtgg ccggcgcgac gacggccgag
     7501 taattggcca tcatcggcac gatgagggcg gccatctcct cggcaaggcc gttggcgagg
     7561 ctcggcgcat ccacgaggag aaccttggcg gcgccatcga tcttggcggc agcttccgcg
     7621 gcggggttgc aaccagcgcc ggcgacgagc acgtgaacgt cacccccgag ggccttggcg
     7681 gccgacatgg ccttgagcgt ctgggctccg acggcggcat tgttatggtc ggctacgaga
     7741 aggacggcca tcagatgatc ccccgatcct tgagcttcgc gacgagctcg gcgccgctcg
     7801 cgaccttgat accggccttg cgtcccgcgg gctccgtcgt cttgagaacc ttgagacggg
     7861 ccttgacctc gactccgagg tcggccggct tcttcacatc aagcggcttc ttcttcgcct
     7921 tcatgatgtt cggcagcgag gcatagcgcg gctcgttgag gcgcaggtcc gtggtgatta
     7981 tcgccggcag cttgagcttc agcgtctcga gaccgccatc cacttcgcgg gtcacggcga
     8041 gcgcgccgcc ttcgacagcg atcttcgagg cgaaggtgcc ctgtgaccag ccgaggagcg
     8101 cggccagcat ctggccggtc tggttgcagt cgtcgtcgat cgcctgcttg ccgaggatga
     8161 cgatgtccgg cttctcggca tcgacgaggg ccttgagcaa cttggcgacc gtgagcggct
     8221 caatgccgtc gtcgcccgtc tcgacctgga tgcctcggtc tgcgcccatg gcgagcgctg
     8281 tgcggatcgt ctcctgtgtc ttggcagagc cgatcgagac cgcaatgatc tccttggcga
     8341 cgcccttctc cttgagccgg atcgcctcct cgacgccgat ctcgtcgaac gggttcatcg
     8401 acatcttgac atttgcgagc tcgacgcccg agccatcggc cttcacccgg atcttcacgt
     8461 tgtagtcgac cacccgcttg acgggcacga ggatcttcat gactcgacag ctcccatgga
     8521 cgccgcgagg cgccgaaatg atctccgcac tgctgtcgcc gcccctggcg gcgcgcggcg
     8581 gcaccctaca gcacgctgcc ccttgttgca gcgcggcccc ttccacgcga gttgaggcaa
     8641 attgccccgt ttgctcccgg gtgtcatctc tttcgcagtt gcgagatgaa actatgcagc
     8701 tccaatccga gagtcaatca gctgattgct ggcccgtgac gcctgcatgt cggagcactc
     8761 gttccggcgg ctgcggtcgc gacgcctcac cgggcacgcg gccctggcag cgctgccgag
     8821 cgaccggtgg ctgccgccga cgctgggcag atgacgttcg gaccgatcga cacctcgggg
     8881 cgcccgcccg cgcagagcgg tcgccgcacg agggttggct cgccgagctt gcgactgcgc
     8941 ggtccaccag caggcggcag cacccccgta tagcgataat ggccggtgat ctgatcggca
     9001 aaaagagcgt cctcgagttg cgggccccag ccgcgattgt ggacgatcat cggccgaccc
     9061 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncccgccg gtgatcgatg
     9241 ctgcgatcgc cactgccggc ccggctctca tgaacgaggc gctgcagatc gacgccgacg
     9301 ccccggtaag ccctgatcac gaggtcggtg caggcgccgt ataggcgcgg cacgtcgccg
     9361 agcgggtatg aaatgcgcac atagcgagcg tcatagatgc cgaactcgcg tgtctccgca
     9421 acagccgctg ccgccagcgc cataccgaac gtggcgccat cggacggcgc gggtctggcg
     9481 tgccccgact tcatcccggt gagatgcgaa aggacaagcg ggctcggctc gcagcgctcc
     9541 aacgtggcat cggccggcac agccaccctt ggcgcatcga ctctgggcgc gtccgacgtc
     9601 ggcggtcgga cggtcggcac cggtggtgcg tcgatcggtg cggcggcggc gagatccacc
     9661 ggcggcgtct cagctgaagg cagcggggcc tttgcgagcg gcggcaaatg gttcggccag
     9721 tgcttcggcc gcggccccat cagaaatccc ggaaggtgcc gaagaataag gtcatgcggc
     9781 tgcgcccggc ggtcggcgga cgccggcgtg tcaggcgtcg ggaagccgct cgcatcgagg
     9841 gcggctgtcg cgatttccgg cggcagcggt ggcggcagcg cgccgacgtc gacggttgcg
     9901 gtcgcgacct cttccccaag cggcggcggc aattgtccga gatcgagccc gccaatcgcg
     9961 acctcggcgg gcgccggttg cggaagcagg ccgacgtcga tagctgcgac ggggacctcg
    10021 cccctcagga cgtccggcag ccggccgatg tcgatgctcg caatggtcgt ctcggctccc
    10081 atnnnnnnnn nnggcggcag cgcgttcacc tcgagactgg cagttgccgt ctcggggcca
    10141 agtgccgggg ctggaacgat agctggtggc gcgaccgaga gcgtcggccg ctctggatcg
    10201 aggacggcgg cggcgcgggc gccctccaag gcaggtcccg gttgcagggc caggaaggcc
    10261 gtgacgatga ctgccggcag agcgacaagg ctcgcaaggc gccgctcgcg accgcgcccg
    10321 agctcggggg agctggcggc gccacccggc tggccggtcc gcgaccgatc gccgaggctt
    10381 t
//
LOCUS       DAORNH010000152        10357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4791_length_10357_cov_2.599981, whole genome shotgun sequence.
ACCESSION   DAORNH010000152 DAORNH010000000
VERSION     DAORNH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4791_length_10357_cov_2.599981"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK264_15025"
     CDS             complement(<1..270)
                     /locus_tag="PK264_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77219.1"
                     /translation="MPSDILVVDDEADIRELVAGILEDEGHKTRLARDADEALRAIEE
                     RRPHLVVLDIWLQGSRLDGLEVLTHIKKQHPDLPVVIISGHGNIET"
     gene            complement(308..>511)
                     /locus_tag="PK264_15030"
     CDS             complement(308..>511)
                     /locus_tag="PK264_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77220.1"
                     /translation="TKGVKGTGLGLAIVQKITEHHGGRLDLEDAPAGPGRRRGALVRL
                     TLPAKRAQDAAPSAAPALAAETV"
     assembly_gap    512..552
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<553..2583)
                     /locus_tag="PK264_15035"
     CDS             complement(<553..2583)
                     /locus_tag="PK264_15035"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRD77221.1"
                     /translation="MRSDDKEIELDGTTQSGLGLPVMSAKTVRIGAALVIAALLSSLT
                     TFLVITGLTPVPTTDIVIYGLFFLNFVLICSIAGLLGWQAWGLARAWRNKVAGARIHV
                     RIVALFTVIAALPALLLAAAATTTFSRALDNAFNNRTRTIVNHSLDVARAYFVEHGQV
                     IRTDIVNMGKDIDDTAARITGDPSQSRPLIDLVTGQVGLRELSIAAIIDGKGTVVVQA
                     ASDTRVTYSPPSERIIAVAEGGNVPVLYPTDSNRVAAITKLANLKGLYLYGARSVDPR
                     VVAHVRAAQDGVAEYEFLRRNRGIHKFLHGIMYFTLSLSAVMAAIWVGLWFASRLVAP
                     IRRLIAAAQRVATGDLSVQLPIYRGEGDLRRLSKNFNNMTTELARQRRDIDEAREKIE
                     ERNRFMEAVLAGVSAGVIGIDAQRRITLVSQSAEKLLGRPLSPHVGAPLTTVVPELEP
                     ILAELGEGRIRPPDPIKIEVDGKERTIAVQVTSEADDGVSRGSVVTFDDVTELMTAQR
                     SSAWGDVARRIAHEIKNPLTPIQLSAERLKRKYAGAIREDRETFEKLTDTIVRQVEDL
                     KRMVNEFAEFARMPKPQIQLADLRDAVQEPVIQYRETHPAIDYALFLPKQPVLTACDQ
                     RMIAQAVTNLTKNATEAIETAMSTPDKDPDYKGRVETHLKVDGSRILIEVIDN"
     gene            2922..>3304
                     /locus_tag="PK264_15040"
     CDS             2922..>3304
                     /locus_tag="PK264_15040"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRD77222.1"
                     /translation="MLDRRIIETSVAAWLAEDVGMGDLTSELVIPAHATGHFQIEARV
                     DLIVAGMEVALAVFRQAEPRAVIEVVARDGERVTRSATLARVTGPARGLLTAERTALN
                     ILQRMSGIATLTAEYVARISHTRARL"
     assembly_gap    3305..3339
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3340..>3615
                     /locus_tag="PK264_15045"
     CDS             <3340..>3615
                     /locus_tag="PK264_15045"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRD77223.1"
                     /translation="EKHAVVLGGGSNHRLRLDNGIMIKDNHIAVCGGISAALARARAG
                     VPRLTKIEVECDRLDQVHEAVAAGADMIMLDNMTLADMRKAVGFVAG"
     assembly_gap    3616..3692
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3782..>4297
                     /locus_tag="PK264_15050"
     CDS             3782..>4297
                     /locus_tag="PK264_15050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRD77224.1"
                     /translation="MALFCSANDVAVRQFGDVAEAFGAAVATAGYTLVYGGGARGLMG
                     IAAKAAHAAGGLVHGVMSQALRDREGALEAIGTLEIAGTLMDRKARMIALANAFVALP
                     GGIGTLDEFTDVVTLNDIGTIAKPIIICNAKGFWTPLVGLMQHLDGHGMLRAGTRRAV
                     IVADGVATTIER"
     assembly_gap    4298..4345
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4377..5369
                     /locus_tag="PK264_15055"
     CDS             4377..5369
                     /locus_tag="PK264_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRD77225.1"
                     /translation="MIGRRLKALSAAVGGAAVLTVMSGALAQDKTLKVTLAGGSVGGA
                     WSAMGSAIGEAIRRQSPGAAFTYEPGRDAANVQLVAQKRVELAIAHAQIARRAAAGEA
                     PFAAKIGNVKGVAMLDKLATLQVIAAHAFGAASVEEIGAKKLKARVALNVRGSLMAIT
                     GEELLKAAGAPVKDLEAAGGKVHYVAYNDGLEMLKSGQVDIVVNMLEFPSRQLVNATR
                     DLKVRFLPMSAATAGALNAALGTSSMSIPAGTYPFQSSAIETISGHVVLLAADETSAE
                     VVATTVRAMLKNFDYLKAAHAALGRLTPADLPLTPGVELHPAAAAVYKEAGLLK"
     gene            5385..7307
                     /locus_tag="PK264_15060"
     CDS             5385..7307
                     /locus_tag="PK264_15060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HRD77226.1"
                     /translation="MLAITRHLGAGLSRELGTLERAIVVAIGVAVVGGMLAVVLGFEV
                     DRQRVMCAVLGLMMVVAFLTTNGSPSRPRRGSAAGCALALGSAIVAGYFTALSPTHQL
                     RLPMVTPLSALESGAAVVLLLLILEATRRTMGMSLLVVIAGFLAYAVFGHLIEGSFSH
                     RRLTGEEILDQLVFTPNGVFGSAMAVATFLVLIFVAFGSILERFGGGDFFFDLSAALV
                     GRQPGGPAKVAIISSGLYGTISGSPTADVVTTGAFTIPLMIRVGYSRVYAAAIEAVAS
                     TGGAILPPVMGTAAFLMSDVTGISYGEIAKASIIGALLYYFALFVATDAMARRGNIGA
                     FPSERIQAVGAVLRRDWVFFIPIAILIWFVFNGDRPTYGAGWAIIAMAPIALMRSANP
                     RALLVALGGALSDSVRRAAPVVVACAVAGIVVGSLSITDLSGKFSSMLFGFAPGDLIY
                     VLVIAALVTLILGMGMPTPAVYVLAAVLLAPAIINLGVSKLAAHLFIVYYASMSAITP
                     PVAVAAFAAASIAEDNPIRIGLASCRLALIGFILPFLFVYRPALLMQGSALEIASVVL
                     ATFAGVTALAAGWQGYFYGRLEAARSAVMALGGVALVWPGWRINALGAAPVALVIVGQ
                     ILLRRPGPIGDGGRHA"
     gene            7300..8205
                     /locus_tag="PK264_15065"
     CDS             7300..8205
                     /locus_tag="PK264_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HRD77227.1"
                     /translation="MHEVSELEERFGRAGAVRFSISPLGGPIVTLSAAESEVDIALKG
                     AQVLSWRRGGREMLWLSPAGRLDSAKPLRGGIPVCWPWFGPHPDDNMKPAHGFVRTAA
                     WRPAASSAGPTQAQISLVIATGAGEAGLWPHAARATLTVTLSEQLSLDLATTNRGTAP
                     LPLTAALHTYFAVGEIAESSIEGLSGHDYMDKLDGDRVKSQAGRIIIDREVDRIYLGP
                     APKIELSDRRLGRRIIVAGRGSASTVVWNPWIDKAARLGDLGPDGWRRMLCIETANAG
                     PDRRMVAPGESTMLGVTYSVIDALR"
     gene            8257..8625
                     /locus_tag="PK264_15070"
     CDS             8257..8625
                     /locus_tag="PK264_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HRD77228.1"
                     /translation="MNTEAKYVDGFVLPLPRKNIMAYKAFSEKYGRIWKEFGAIDYVE
                     CIADDVKPGKLTSFPQSVLLKDDEVVVFAWITYKSKADRDRVNAAVMSDERLEGMDSA
                     SMPFDGKRMFFGGFEVLVSL"
     gene            8736..>10357
                     /locus_tag="PK264_15075"
     CDS             8736..>10357
                     /locus_tag="PK264_15075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRD77229.1"
                     /translation="MAPSRQSVSLSTGSLGESAAQPNAIAAGGSSAGGADLGAMPEWD
                     LGDLYASPTDPRIAQDLADGARRARRLKDDYQGRIVALATDGARLAEPILEYERLADL
                     LGRLGSYAGLLYAANTADPERAKFYGDLQDKLTTISTDLTFLELELNQIPEDTLANAL
                     KSPQLARLAPWLLDLRKEKPYQLDEKLERLFLEKSATGASAWGRLFNETVTSLKFEIE
                     GEAAPLGLEPTLNFLMHPEEPRRAAAEALSKVFKDNVRLFTLITNTLAKDKEISDRWR
                     GFKDVADSRHLSNRVEAPVVAALVDAVRASYPALSHRYYAMKARWLGMKRLSFWDRNA
                     PLPNRPDQTISWPRAREIVLDAYRGFAPEMADIARPFFDRNWIDAPIRPGKSPGAFSA
                     STVPSAHPYILMNYQGKARDVMTLAHELGHGVHQVLAAKQGPLLAPTPLTLAETASVF
                     GEMLTFRALLAETRDPAARKAMIAQKVEDMINTVVRQIAFYVFERRVHEARREGELTA
                     EKLGEIWLDVQKESLGPAIDLGPGYENYWVYIG"
BASE COUNT         1577 a         3304 c         3480 g         1795 t
ORIGIN      
        1 ggtctcgatg ttgccgtggc ccgagatgat gaccacgggg agatcggggt gctgcttctt
       61 gatatgcgtc agcacctcta ggccgtcgag gcggctccct tgcagccaga tgtcgaggac
      121 gacgagatgc ggccggcgct cctcgatggc gcgcagcgcc tcgtccgcat cgcgggctag
      181 gcgcgtcttg tggccctcgt cctcgaggat gcccgcaacg agctcgcgga tgtcggcctc
      241 gtcatcgacg accagaatgt ctgacggcat tggtcactcc tcggaagcga tgccttggtt
      301 gcgattttca aacagtctcg gcggcgagcg cgggggcggc actaggtgcc gcgtcctgtg
      361 ccctcttggc cggcagcgtg aggcgaacga gcgcgccgcg ccggcgaccc ggtccggccg
      421 gcgcatcctc gaggtcgagc cgtccgccgt gatgctcggt gatcttctgg acgattgcga
      481 ggccgaggcc ggtgcccttg acgcccttgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nngttgtcga tcacctcgat gaggatgcgg ctgccatcca ctttgaggtg
      601 cgtctcaacg cggcccttgt agtcgggatc cttatccggc gtggacatgg ccgtctcgat
      661 cgcttccgtg gcgttcttcg tgagattggt cacggcctgc gcgatcatgc gctgatcgca
      721 ggcggtcagc accggctgct tcggcaggaa cagcgcgtag tcgatcgcgg ggtgcgtctc
      781 gcgatactgg atgaccggct cctgcacggc gtcgcgcaga tcggcgagct ggatctgcgg
      841 cttcggcatg cgtgcgaact cggcgaactc gttgaccatg cgtttcaggt cctcgacctg
      901 gcgcacgatc gtgtcggtga gcttctcgaa ggtctcgcgg tcctcgcgga tcgcgcccgc
      961 atacttgcgc ttcaggcgtt cggccgagag ctggatcggc gtcagcgggt tcttgatctc
     1021 gtgggcgatg cgacgcgcaa catcgcccca ggctgaagag cgctgcgccg tcatcagctc
     1081 ggttacgtcg tcgaaggtga cgacggagcc gcgactgacg ccatcgtccg cttcgctcgt
     1141 aacctggacg gcgatggtgc gctccttgcc gtcgacctcg atcttgatcg ggtcgggggg
     1201 gcggatgcgg ccttcaccga gctcggcgag gatcggctcg agctccggca cgacggtcgt
     1261 gagcggcgcg ccgacatggg gggagagcgg ccggccgagc agcttttcgg cgctctggct
     1321 gacgagcgtg atgcggcgct gggcatcgat gccgatgacg cccgccgaga cgcccgcgag
     1381 cacggcctcc atgaagcggt tgcgttcctc gatcttctcg cgcgcctcgt cgatgtcgcg
     1441 ccgctggcgg gcgagctcgg tcgtcatgtt gttgaagttt ttcgagaggc gtcggagatc
     1501 gccttcgccg cgatagatcg gcagctgcac ggagagatcg ccggtcgcga cgcgctgggc
     1561 ggcagcaatg aggcggcgga tcggcgccac gagcctgctc gcgaaccaga ggcccaccca
     1621 gatcgccgcc atcacggccg acagcgacag ggtgaaatac atgatgccgt gcaggaattt
     1681 gtggatgccg cggttgcgcc tcaggaactc gtactcggcg acgccgtcct gcgcggcacg
     1741 cacatgggcg acgacgcgtg ggtcgacgct gcgggcgccg tagagataga ggcccttgag
     1801 gttggcaagt ttggtgatcg cggcgacacg gttgctgtcg gtcgggtaga ggaccggcac
     1861 gttgccgcct tccgcgaccg cgatgatgcg ctcggagggc gggctgtagg tgacgcgcgt
     1921 gtcgctggca gcctggacga cgaccgtccc tttgccgtcg atgatggcgg cgatcgagag
     1981 ctcgcggagg ccaacttgcc cggtgacgag atcaataagc ggccgcgact ggctcggatc
     2041 gcccgtgatg cgcgccgccg tatcgtcgat gtccttcccc atgttgacga tgtcggtgcg
     2101 gatgacctgt ccgtgctcga cgaaataggc gcgcgcgaca tcgagcgagt ggttgacgat
     2161 ggtgcgcgtg cgattgttga aggcgttgtc gagcgcccgc gagaaggttg tcgtggccgc
     2221 cgcggcgagg agcagcgccg ggagcgcggc aatcaccgtg aacagcgcga cgatgcggac
     2281 atggattcgg gcgccggcga ccttgttgcg ccaggcgcgg gcgaggcccc aggcctgcca
     2341 gccgagcagg ccggcgatcg aacaaatgag aacgaagttc aggaagaaca ggccgtagat
     2401 cacgatgtcg gtcgtcggga ccggcgttag ccctgtgatc accaggaatg tggtgaggct
     2461 cgagagcagc gcggcgatga cgagcgcggc gccgatgcgc accgttttgg cgctcatgac
     2521 cggcaggccc agtccggact gggttgtgcc gtcgagctcg atctccttgt cgtcactcct
     2581 catcgtcgtg accagctcct gggctctcga acgccgaacg tgcaggccgc cgtcagatcc
     2641 ggcgcggcgg ggtgtgtcct ccgtgcaatg cctccctatc cgcaggaggc gtcgttcgta
     2701 acagcatcaa actgtgacat ttccgcgaca cattcacgaa gtaaccatgt ggcaccggag
     2761 cgtcaagtcg attccccgga gagttgcagc aaagctgtgc gccagtgagc caaccccgaa
     2821 aggcgtgggt cgacgcctgg tcagcccgat tgacagcgcc atgacgatcg cgcgagcttg
     2881 gcgtggatcg tgacctggcg tggtgcggcg cggaggcggg tgtgctggat cggcgaatca
     2941 tcgaaacgag tgttgcggcg tggctggccg aggacgtcgg catgggcgat ctgacgtcgg
     3001 agctcgtcat tccggcgcat gccaccgggc acttccagat cgaggcgcgt gtcgatctga
     3061 tcgttgctgg catggaggtt gcgctcgcgg ttttccgcca ggcagagccc cgtgccgtaa
     3121 tcgaggtcgt ggcgcgagat ggcgagcgcg tgaccagatc ggcgaccctt gcccgtgtga
     3181 ctgggccggc gcgcggcctg ctgacggccg agcgaacagc gctcaacatc ctgcagcgga
     3241 tgtcgggcat cgcgacgctg acggccgagt acgtggcgcg catcagccac acccgggcgc
     3301 ggctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt cgagaagcat gccgtcgtgc
     3361 ttggcggcgg cagcaatcac aggttgcggc tcgacaatgg catcatgatc aaggacaacc
     3421 acatcgccgt ctgcggcggc atatccgcag cactggcgcg tgcgagagcg ggcgtgccgc
     3481 ggctcaccaa gattgaagtg gagtgcgatc gcctcgacca ggtgcacgag gcggtggcgg
     3541 ccggtgccga catgatcatg ctcgacaaca tgacgctcgc cgacatgcgc aaggccgtgg
     3601 gcttcgtcgc agggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggatcac ccagtccgcg gccgccgtcg
     3721 acatcggcct cgacgtggcc atcgaggcat gagcggcgcg ccccagcccg cgaggttgtc
     3781 ggtggcgctc ttttgctcgg ccaacgatgt cgcggtacga cagttcggcg acgtggccga
     3841 ggccttcgga gcagcggtcg cgacggctgg ctacacgctc gtctacggcg ggggcgcccg
     3901 cggactgatg ggcattgccg ccaaggcggc acatgcggcc ggagggctgg tgcatggtgt
     3961 catgtcgcag gcgctgcgcg accgggaggg cgcgctcgag gcgatcggca cgctcgagat
     4021 tgccggcacg ctgatggacc gcaaggcccg catgattgcg ctcgcgaatg ctttcgtagc
     4081 gctgccgggc ggaatcggca cccttgacga gttcacggac gtcgtgacgc tcaacgatat
     4141 cggcacgatc gccaagccga tcatcatctg caacgccaag ggcttctgga caccgctcgt
     4201 cggtctgatg cagcatctcg acggtcacgg catgctgcgt gccggtactc ggcgcgccgt
     4261 gatcgtcgcc gacggcgtcg cgacaacgat cgagcggnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnntcgag ccaatccccg agggagggag gacgacatga
     4381 tcggacgacg gctgaaggct ctttcggcgg cagtcggcgg ggcggcagtg ctgacagtca
     4441 tgtcgggcgc tctcgcgcag gacaagacgc tcaaggtgac gcttgccggc ggctccgtcg
     4501 gcggcgcctg gagtgcgatg ggcagcgcta tcggcgaggc gatccggcgt cagtcgccgg
     4561 gtgcggcctt cacatacgag ccgggccgcg atgccgccaa cgtccagctc gtcgcccaga
     4621 agcgtgtcga gctcgccatc gcccatgccc agatcgcgcg ccgcgccgcg gctggcgaag
     4681 cgccctttgc ggccaagatc ggcaacgtga agggcgtcgc gatgctcgac aagctcgcga
     4741 cgctgcaggt gatcgcggcc catgctttcg gcgctgcgag cgtcgaggag atcggagcca
     4801 agaagctcaa ggcgcgtgtc gcactcaacg tgcgcggctc gctcatggcg atcacgggcg
     4861 aggagcttct taaagccgcc ggcgcgccgg tgaaggacct cgaggcggcg ggcggcaagg
     4921 tgcactacgt cgcctacaac gacgggctcg agatgctgaa gtcgggacag gtggacatcg
     4981 tcgtcaacat gctcgagttc ccctcgcgcc agctcgtcaa cgcgacccgc gatctgaagg
     5041 tccgcttcct gccgatgagc gctgcgacgg ctggggcact caatgcggct cttggcacct
     5101 cgtccatgtc gatcccggct ggcacctatc cgttccagag cagtgcgatc gagacaatct
     5161 cgggacacgt cgtgctgctc gccgccgacg agacgtcggc cgaggtcgtg gcgaccaccg
     5221 tgcgcgccat gctcaagaac ttcgactacc tgaaggcggc gcatgcagca ctcggccggc
     5281 tgacgcccgc cgatctgccc ctgacgcccg gcgtcgagct gcatccagcc gcggcggccg
     5341 tctacaagga ggccgggctc ctcaaataga ggtccggggg aggcatgctg gcgatcacgc
     5401 ggcatctcgg cgcgggactg tcgcgtgagc tcggcacgct cgagcgcgcc atcgtcgtcg
     5461 ccatcggcgt cgcggtggtc ggcggcatgc tcgccgtcgt gctcggcttc gaggtcgacc
     5521 gtcaacgcgt gatgtgcgcg gtcctcggcc tcatgatggt cgtcgcgttt ctcacgacca
     5581 acggctcgcc gtcgcggcca cgacgtggct cggctgccgg ctgtgcgctc gccctcggct
     5641 cggcaatcgt cgccggctat ttcacggcgc tgagcccgac gcaccagctg cgcctcccca
     5701 tggtgacgcc gctctcggcc ctcgagtcgg gcgcggccgt cgtgctgctc ctgctcattc
     5761 tcgaggcgac ccggcgcacg atgggcatga gcctgctcgt cgtcatcgcg gggtttctcg
     5821 cctatgccgt gttcggccat ctgatcgagg gctcgttctc gcaccgccgg ctcaccggtg
     5881 aggagatcct cgatcaactc gtcttcacac cgaacggcgt gttcggctcg gcgatggcgg
     5941 tcgcgacgtt cctggttctg attttcgtcg ccttcggctc gatcctcgag cgcttcggcg
     6001 gcggcgactt cttcttcgac ctgagtgcgg cgctcgtcgg ccgccagccg ggcggacccg
     6061 ccaaggtcgc gatcatctcg tcggggctct acggcacgat ctcgggctcg ccgaccgccg
     6121 acgtggtgac gaccggcgcc ttcacgatcc cgctcatgat ccgcgtcggc tacagccgcg
     6181 tctatgcggc ggcgatcgag gcggtcgcct cgacgggcgg cgcgatcctg ccgccggtaa
     6241 tgggcaccgc cgccttcctg atgtccgacg tcactgggat ttcctacgga gagatcgcca
     6301 aggcctcaat catcggcgcg ctgctctact atttcgcgtt gttcgtcgcg accgacgcca
     6361 tggcgcggcg cggcaacatc ggcgcctttc cgtcagagcg cattcaggcc gtcggcgcgg
     6421 tgctacggcg ggactgggtc ttcttcatcc cgatcgcgat cctcatctgg ttcgtcttca
     6481 acggcgaccg gccgacgtac ggcgcgggct gggccatcat cgccatggcg ccgatcgccc
     6541 tcatgcgcag cgcgaacccg agagcgctcc tcgtggcact cggcggcgcg ctctccgaca
     6601 gcgtccggcg cgcggctccc gtggtcgtcg cctgtgcggt cgcaggcatc gtcgtcgggt
     6661 cgctctcgat aaccgatctc tcgggcaagt tctcgtcgat gctgttcggc tttgccccgg
     6721 gcgacctcat ctatgtgctg gtcatcgcgg cgctggtgac gctgatcctc ggcatgggca
     6781 tgccgacgcc ggcggtctat gtgctcgccg ccgtcctgct cgccccagcc atcatcaatc
     6841 tcggcgtgtc gaagctcgcc gcccacctct tcatcgtcta ctacgcctcg atgtcggcca
     6901 tcacgccacc cgtggcagtg gcggcctttg cggcagcctc gatcgccgag gacaatccga
     6961 tccggatcgg gctcgcctcg tgccgattgg cgcttatcgg cttcattctg ccgttcctgt
     7021 tcgtctaccg gcctgcgctg ctcatgcagg gatcggccct cgagattgcg agtgttgtgc
     7081 tcgcgacgtt cgcgggcgtc acagcgctgg ctgcgggctg gcagggctac ttctacggcc
     7141 ggctggaggc cgcgaggtcg gcagtcatgg cactcggcgg ggtagcgctg gtttggccgg
     7201 gctggcggat caacgcgctc ggcgcggccc cggtcgcgct cgtcatcgtc gggcagatcc
     7261 tgttacgccg gccggggccg atcggggatg gagggcggca tgcatgaggt gtcggaactc
     7321 gaggagcgtt tcggtcgcgc cggggccgtg cgcttctcga tcagcccgct cggcggtccg
     7381 atcgtgacgc tctcggccgc ggagagcgag gtcgacatcg cgctcaaggg cgcgcaggtg
     7441 ctctcgtggc gccgaggcgg gcgcgagatg ctttggctgt caccggcggg tcggctcgat
     7501 tccgccaagc ctctgcgcgg cggcattccg gtctgctggc cgtggttcgg cccgcatccc
     7561 gacgacaaca tgaaacccgc gcatggcttc gtgcgcacgg ctgcctggcg gcctgccgcc
     7621 tcgtcggccg gtccgacgca agcgcagatt tccctcgtga ttgcgaccgg cgccggagag
     7681 gccggtctct ggccgcacgc cgcccgggcg acgttgacgg ttacactctc tgagcagctc
     7741 tcgctcgatc ttgcgaccac caatcgaggg accgcgccgc tcccgctgac cgccgcgctg
     7801 catacctatt tcgcggtcgg cgagattgcc gagtcgtcca tcgaaggcct ctcgggtcac
     7861 gactacatgg acaagctcga cggcgatcgc gtcaaatccc aggcaggtcg catcatcatc
     7921 gaccgcgagg tcgatcgcat ctaccttggg ccggcaccga agattgaact cagcgatcgc
     7981 cggctcggac ggcgcatcat cgtcgcgggc cgcgggagtg cctcgaccgt cgtgtggaat
     8041 ccgtggatcg acaaagccgc gcggctcggc gatctcgggc cggacggctg gcggcgcatg
     8101 ctctgcatcg agacggcgaa tgccggtccg gaccggcgga tggtcgctcc gggagagagc
     8161 acgatgctcg gtgtcacata ctcagtgatt gatgcgctgc gttgatcgtg ctccatgtgg
     8221 gggccacgtc gggcgtggga tacatggaga agacccatga acaccgaagc aaagtacgtt
     8281 gacggcttcg tgctgccact gccacgcaag aacatcatgg cctacaaggc gttctcggag
     8341 aagtacggcc gcatttggaa agaattcggc gcgatcgact acgtcgaatg cattgccgac
     8401 gacgtgaagc cgggcaagct cacctcgttt ccgcagtcgg tgctgctcaa ggacgacgag
     8461 gtcgtcgtgt tcgcctggat cacctacaag tcgaaggcgg accgggatcg cgtcaacgca
     8521 gcggtcatga gcgacgagcg cctcgagggc atggacagcg cgtcgatgcc cttcgacggc
     8581 aagcgcatgt tcttcggcgg cttcgaggtg ctggtgtcgc tttaggcggg actacgggtg
     8641 gccggtggcc aatcaagcct gcccagcctg gttgcagcat gcgcctccaa ccactagatg
     8701 aggtgcacgc ttcagattct ccaggaatgg cacccatggc accgagccgt cagagcgtct
     8761 cactgtccac cggttccctc ggcgagagtg ccgctcagcc gaatgccatt gcggcaggtg
     8821 gatccagcgc gggcggcgcc gatctcggag ccatgcccga gtgggatctc ggcgatctct
     8881 atgcctcgcc gaccgatccg agaatcgcgc aagacctcgc tgacggtgct cgccgggcga
     8941 ggaggctcaa ggacgattac cagggccgga tcgtcgcgct tgcgaccgat ggcgccaggc
     9001 tcgcggagcc catcctcgaa tacgagcggc tcgccgatct cctaggccgg ctcggctcct
     9061 atgccgggct tctctacgcg gccaacacgg ctgatccgga gcgggcgaag ttctatggcg
     9121 acctgcagga taagctcacg accatctcca cggacctgac gttcctcgaa ctcgagctca
     9181 accagatccc cgaggacacg ctcgccaacg cgctgaagtc gccgcagctt gcccggctgg
     9241 cgccttggct tctcgatctg cgcaaggaga agccctacca gctcgacgag aagctcgagc
     9301 ggctcttcct cgagaagtcc gcgacagggg catcggcctg gggccgcctc ttcaacgaga
     9361 ccgtgacgag cctcaaattc gagatcgagg gcgaggctgc tcctctcggg ctcgagccga
     9421 cactcaattt cctgatgcac cctgaggagc cgcggcgggc cgcggccgaa gcgctctcga
     9481 aggtgttcaa ggacaacgtc cgcctcttca cgctgatcac caatacgctc gccaaggaca
     9541 aggagatctc cgaccgctgg cgcggcttca aggacgttgc cgacagccgg cacctgagca
     9601 accgcgtcga ggcgcccgtg gtggcggccc tcgtcgacgc ggtgcgggca agttatccgg
     9661 cgctttcgca tcgctactac gcgatgaagg cgcgctggct cggcatgaag cggctgagct
     9721 tctgggatcg caatgcgccg ctgccgaatc gtcctgacca gacgatttcc tggccgcgtg
     9781 cgcgcgagat cgtgctcgac gcctatcgcg gcttcgcgcc ggaaatggcg gacatcgcga
     9841 ggccgttctt cgaccgcaac tggatcgatg cgccgatccg tcccggcaag tcgccgggcg
     9901 ccttctcggc gtccacggtg ccctcggcgc acccatatat tctgatgaac taccagggga
     9961 aagcccgcga tgtgatgacg ctcgcccacg agctcggcca cggcgtgcac caggtgctcg
    10021 cggcgaagca agggccgctg ctcgcaccga caccgttgac gctcgccgaa acggcctccg
    10081 tgttcggcga aatgctgacc ttccgcgcac tcctcgccga gacgcgcgat ccagcggccc
    10141 gcaaggcgat gatcgcgcag aaggtcgagg acatgatcaa tacggtcgtg cgccagatcg
    10201 ccttctacgt attcgagcgc cgcgtgcacg aggcgcgccg cgagggcgag ctaaccgccg
    10261 agaagctcgg ggagatctgg ctcgacgttc agaaggagag cctcggaccc gcgattgatc
    10321 tcggccccgg ctatgagaac tactgggtct acatcgg
//
LOCUS       DAORNH010000153        10316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4815_length_10316_cov_2.692038, whole genome shotgun sequence.
ACCESSION   DAORNH010000153 DAORNH010000000
VERSION     DAORNH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4815_length_10316_cov_2.692038"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..892
                     /locus_tag="PK264_15080"
     CDS             149..892
                     /locus_tag="PK264_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77230.1"
                     /translation="MTAEFDARTAEQRRVWDEKPTLRLIYADFHRRLEAACPPGRLLD
                     IGGSAHFKDYRPDVVSLDILHFPGIDVVADAHHLPFADGVFAGIVMLDVLHHLQRPVV
                     FLAEAARVLATGGRLAMIEPGMSRIARHVYSRFHHEPVDMRADPFAVDRRQSGDDPWD
                     SNQAIATLMFGSSERIARVEAAVPSLKVVSAEWLSLLAYPLSGGFQRWSLMPRRLTAP
                     MLAIEERLRHAVGTTLGFRQMVVIEKRRV"
     gene            1257..1751
                     /locus_tag="PK264_15085"
     CDS             1257..1751
                     /locus_tag="PK264_15085"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HRD77231.1"
                     /translation="MPPCDPRRVDNGPPPDLQAAETCDKGHGCFETRRIAVTAEAVEH
                     LDWPGLAQFARLTRTRQVAGKTSVETVYMVVNLPPGEADPVRLPALSLAHWGVENRLH
                     YVRDISMDEDRCRVRSGARALAGVRNTALSIIRRLGRSDREARESVREDRAEAIRAVT
                     GRNL"
     gene            1845..2132
                     /gene="paaB"
                     /locus_tag="PK264_15090"
     CDS             1845..2132
                     /gene="paaB"
                     /locus_tag="PK264_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HRD77232.1"
                     /translation="MTEKPMPLWEVFIRARAGVAHKHAGSIHAADATMALQAARDVYT
                     RRGEAVSIWVVPSAAITASDPADKAELFEPTGSKIYRHPTFYEIPDEVGHM"
     gene            2180..2857
                     /locus_tag="PK264_15095"
     CDS             2180..2857
                     /locus_tag="PK264_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD77233.1"
                     /translation="MADARTESETDAPAKVAGRQLVYFADPMCSWCYGFSPVIGEIAR
                     RFEGRMPVRIVMGGLRAGNERPMRDADRDYIRSAWTRVAAASGQPFDHAFFERESFVY
                     DTEPACRSVVTMRRRAPERALEFLTRVSRAFYAENRDTTKASELAKLAAEFGEDADAF
                     ELAMLDPEMRNATLRDFLLAQQSGVSGFPCLVAGREADGYALVTSGFATLDTLIEPIE
                     TWYAGQA"
     gene            2885..>3462
                     /locus_tag="PK264_15100"
     CDS             2885..>3462
                     /locus_tag="PK264_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD77234.1"
                     /translation="MSKAVLWSRDARGVATVTFNRPEVNNAYNGDLIQGVLDAMDDLG
                     GQPGLRVVVLKGNGKHFQAGADLKWINSVRPGSHEANVAVSRATAEAVDRLNRLPVPV
                     VALVQGGCFGGGTGIVAACDVVIAADNALFSIAEVRWGLHAGIIIPQLNDAMGVRQVR
                     RYALTGERFGADEARRLGLVHEVVALADLEATG"
     assembly_gap    3463..3472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3473..3677
                     /locus_tag="PK264_15105"
     CDS             <3473..3677
                     /locus_tag="PK264_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD77235.1"
                     /translation="VRQILENSPDAIAKTKSHILESAWGGFGQTTFNELVDSHAKARQ
                     SPAAAEGLASFAEKRSAKWAPKP"
     gene            3674..4375
                     /gene="pyrF"
                     /locus_tag="PK264_15110"
     CDS             3674..4375
                     /gene="pyrF"
                     /locus_tag="PK264_15110"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRD77236.1"
                     /translation="MRRGAERIFVALDVPTVEAARALVDTLGDSVGGLKIGLELVMGG
                     GLKLAEELAREGRRVFLDMKLLDIPNTVEGAAANAAEAGVAYLTVHGTDRKTLDAAVK
                     GRGNSKTKLLAITVLTSLGSADLREQGSTMAVEELVVHRARLANAAGFDGVVASPLEI
                     AAIRAAVGQSLEIVTPGVRPAGAARGDQTRVMTPAEAIRAGATALVVGRPITAVADPR
                     AAALAIGAEIEAALG"
     gene            4454..5104
                     /locus_tag="PK264_15115"
     CDS             4454..5104
                     /locus_tag="PK264_15115"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020049159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD77237.1"
                     /translation="MTSYRLHCFALSGSAYKVALYLNCAGLKWEAVPVDFLKGQTRDP
                     KWRDEINDMGEAPVLEADGKRYSQSGAILSWLAETTVKFVPNGADERYDALRWILFDN
                     HKFTSYFATHRFLNSIAPQAPDPAVLGFLKTRVDAAFAIVEKHLATRKFMLGAKPTIA
                     DFSLIGYMYYPSEETGYDLAKSHPAIYAWTQRMRFLPGWKGPYELMPGVRPELRKG"
     gene            complement(5140..5631)
                     /locus_tag="PK264_15120"
     CDS             complement(5140..5631)
                     /locus_tag="PK264_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77238.1"
                     /translation="MIARSKSSPWGFLVALPMAFAFLTVGTLAMNLLELPQRVGIFVA
                     PPSWIVGAVLAAFAVILFLVGIVELARWLSPAVEITVDANGISTFGLLGAERLSWNDV
                     NSLETYGPLVVVGTRRRRRNGTSQLVIDTNRLDASPDQISRIISAHRSDLVARPIAAE
                     RVS"
     gene            complement(5801..6565)
                     /locus_tag="PK264_15125"
     CDS             complement(5801..6565)
                     /locus_tag="PK264_15125"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD77239.1"
                     /translation="MSLFDLKGRVAVVTGGNGGIGLGMARGLAKAGANIMIAGRKAAK
                     SKGAADELAKLGVKTAVVEVDVANDASCKAMIAETVKTFGRLDILINNAGINNRKQPE
                     TYSPAEWREVIAINLDGAFFCSHAAHPHLKKAGGGKIINIGSMMSIFGAPFATPYAAS
                     KGGIVQMTKALATAWAKDNIQVNAILPGWIDTDLTKTAREVIPTLHESVLKRTPAARW
                     GGPNDFSGIAIFLSSHASDFITGAAIPVDGGFSVQG"
     gene            complement(6647..8032)
                     /gene="gltX"
                     /locus_tag="PK264_15130"
     CDS             complement(6647..8032)
                     /gene="gltX"
                     /locus_tag="PK264_15130"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRD77240.1"
                     /translation="MSVKVRFAPSPTGRLHVGNIRPAVLNWLFQRRHGGTFLLRLDDT
                     DQGRSTAAFADGIVEDMRWLGLSWDAFLRQSDRLERYAAAAERLKAAGRLYPCYETAD
                     ELDRRRKRQLARGLPPIYDRAALRLTGAETAKLAAEGRRPHWRFRLTNSSGPDDLAPR
                     PTPVAWDDLIRGHQTVDAGSLSDPVLVREDGTFLYTFTSVVDDIETAITHIIRGEDHV
                     TNTGVQIQLFEALGARPPAFAHHSLLVQADGSALSKRLGALSIEGLRKSGLEPEAVVC
                     HAALVGTSDAIEPRATLDDLAKSFDLGKISRAPGRFDENDLHALNARLLHMLPFERVS
                     ARLAALGVSGGAPFWLTVRGNLAVLADAKRWHDIVYGPIAPTVESPDVLKAAADRLPA
                     APWDADTWSPAGWSAYTAAIKAATGASGRALFHPLRIALTGEEKGPELRALMPLMGRE
                     RVAARLRVAQV"
     gene            complement(8095..9264)
                     /locus_tag="PK264_15135"
     CDS             complement(8095..9264)
                     /locus_tag="PK264_15135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD77241.1"
                     /translation="MHEALMTNEQREIRDAIQKICARFDDAYWLEKERKGGFPHDFHA
                     ALADAGWLGIAMPEAYGGAGLGITEAALMMQTIAESGACLSGASAVHMNIFGLNPVVV
                     FGSEEQKKRFLPPLIKGTDKSCFAVTEPNVGLDTTKLKTRARRDGNRYRIDGQKIWIS
                     TAQVANKVLILTRTTPAEEVKRPTEGLTLFYTDLDRKYVEVREIDKMGRKAVDSNQLF
                     IDGLPVPIEDRIGEEGRGFEYILHGLNPERILIASEAVGIGRAAIRKAAQYAKDRVVF
                     GRPIGENQGIQHPLARCWANLEAANLMIFRAAELYDKGLPCGIEANAGKYLAGEAGFE
                     ACETAILSHGGMGYSKEFHVERYLREVLIARIAPVSRELVLSFLAEKALGLPKSY"
     gene            complement(9398..>10316)
                     /locus_tag="PK264_15140"
     CDS             complement(9398..>10316)
                     /locus_tag="PK264_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HRD77242.1"
                     /translation="KADFLLENFRPGTMEKWGCGYEDLAKINPRLIMIRVSGFGQTGP
                     MAPRAGHDINYIALAGVLGAVGRKGAPPTMPLNLVGDFGGGGMLLAVGVLAALLEASR
                     SGKGQVVDAAMVDGAALLMAGIHGLYSQGTWKDERGVNILDTGAPWYEVYETSDGKWV
                     SIGSIEGRFYEDLLARLGLAGEKLPKQWDKSGWPMLRERFAAAFKTKSRDAWCQVFEG
                     SDACFAPVLTMAEVEAHPHNRERKTFLRVDGVMQAAPAPRFSRTAAKAGAASRGVGTD
                     TDAVLGAAGFAPERIKDLIARGVVGTAKS"
BASE COUNT         1661 a         3419 c         3508 g         1718 t
ORIGIN      
        1 agtgcgacga ggctgttcgc ctctttcggg cggcgtgagg cctcatgaat ccgaatcgca
       61 acccaatcag ctctcacctt cacccccaag gggcgaggga agtcgtgtgt cgatgctcga
      121 tgaccaatcg ccgtgttggt tgctccgcat gacagccgag ttcgacgcgc ggacggccga
      181 gcagcggcgg gtgtgggacg agaagccgac gctgcgcctg atctatgccg atttccatcg
      241 ccggctcgag gcggcctgtc cgcccgggcg cctcctcgat attggcggct cggcgcactt
      301 caaggactac cggcccgacg tcgtctcgct cgacatcctg cactttccgg ggatcgacgt
      361 cgtcgccgac gcgcaccatc tgccgttcgc cgacggcgtc ttcgcgggca tcgtcatgct
      421 ggacgtgctg caccacctgc agcggcccgt cgtctttctc gcggaagctg cgcgtgtcct
      481 cgcaaccggc gggcggctcg caatgatcga acctggcatg agtcgcatcg cgcgccacgt
      541 ctacagccgc ttccaccatg aacccgtcga tatgcgcgcc gacccgttcg cggtcgaccg
      601 gcgccagtcg ggcgacgatc cctgggacag caaccaggcg atcgccacgc tgatgttcgg
      661 ctcgtccgag cggatcgcga gggtcgaggc ggcggtgcca tctctcaagg tcgtcagcgc
      721 cgagtggctg agcctcctcg cctatccact ctccggcggc ttccagcgct ggtcgctgat
      781 gccgcgccgg ctgaccgcgc cgatgctagc gatcgaggag cgattgcgcc atgccgtcgg
      841 cacgaccctc ggattccggc agatggtggt gatcgagaag cgccgcgtgt gagaggagcg
      901 agggagcgcg gcgcgcgcgg gcgattgctg ctcgcccgtc ccacttcgat ggagctgggg
      961 caagacattg cgggtcagcc gtcgcctcga cgacaaggcg ctccatgtgt tctccgcgtt
     1021 ctcgaccgat ctcggtgctg ttgttggcga cctcgtcgtc gcccctgatc agaccgatta
     1081 cggctgaggt cgtgctgttg aagggtctgc ccatagaggc gccatcgtca caggacacgc
     1141 gatcttcacg caacgcgcga tctcccagca tatccgtgac cgcgatggcc acgacctgta
     1201 cgtggtcaag gacatccagc aggccctgaa agccgggatc gccgaaggct tcggtgatgc
     1261 ccccctgcga tccacgtcgc gtcgacaacg ggccgccgcc tgacctccag gctgccgaga
     1321 cctgcgacaa gggccacggc tgcttcgaga cgcggcgtat cgccgtcacc gccgaggccg
     1381 tcgagcacct cgactggccg ggcctcgccc agttcgcacg actgacgcgc acacggcagg
     1441 tcgcgggcaa aaccagcgtc gagacggtct acatggtcgt caacctgcca cccggcgagg
     1501 ccgatccggt acggctgccg gccctctctc ttgcgcactg gggggtcgag aaccgtctcc
     1561 actacgttcg cgacatctcc atggacgagg accgctgccg tgtccgctcc ggtgcacgcg
     1621 ccctcgcagg cgtccgcaat accgccctct ccatcatccg aaggctcggc cgctccgacc
     1681 gcgaagctcg ggagagcgtc cgcgaggacc gcgccgaagc tattcgcgcg gtcaccgggc
     1741 ggaatctttg aattgtgcta ccgcatgtgg gcgatgcgat tgcgcggacg cggtgccttc
     1801 gcctagtctc agacgccacg cactccggcc cgaaggtgtc agtcatgact gagaaaccga
     1861 tgccgctgtg ggaggtgttc atccgcgccc gcgcgggtgt cgcccacaag cacgccggca
     1921 gcatccatgc ggctgacgcc acgatggcgc tgcaggccgc ccgtgacgtc tacacgcgcc
     1981 gtggcgaggc ggtctcgatc tgggtggtgc catcggcagc gatcacggct tcggaccctg
     2041 ccgacaaggc ggaactcttc gagccgacgg gctcgaagat ctatcgccat ccgacctttt
     2101 acgagatccc tgacgaggtc ggtcacatgt gagcggccgg caaggcgact tggcgagaca
     2161 aacgttcgac agaggaccca tggctgacgc gaggactgaa tcagagacgg atgcgccggc
     2221 caaggtcgcc ggccgccagc tcgtctactt cgccgacccg atgtgctcgt ggtgctacgg
     2281 cttctcgccc gtgatcggcg agattgcgcg ccgcttcgag ggccgcatgc cggtgcgtat
     2341 cgtaatgggc gggcttcgcg ccggcaacga gcgccccatg cgcgacgctg atcgtgacta
     2401 tatccgcagc gcctggacgc gcgtcgcggc ggcctcgggc cagccattcg accacgcctt
     2461 cttcgagcgc gagagctttg tctacgacac cgagcccgcc tgccggtcgg tggtgaccat
     2521 gcgccgccgc gcgccggaga gggcgctgga gtttctcacg cgcgtcagcc gcgcctttta
     2581 cgccgagaac cgggatacga cgaaagcaag cgagctcgct aagcttgccg ccgagttcgg
     2641 cgaggatgcg gacgccttcg agctcgccat gctcgatccc gagatgcgca atgcgacgct
     2701 ccgcgatttc ctgctcgcgc agcagtccgg cgtctccggc ttcccctgcc tcgtcgccgg
     2761 gcgcgaggca gacggctatg cgctcgtcac gagtggcttt gccacgctcg atactctcat
     2821 cgagccgatt gagacgtggt atgcgggcca agcctgatcg acatcgagat gcgagacgaa
     2881 ccgcatgagc aaagccgtcc tctggtcgcg agatgcccgt ggcgtcgcga ccgtcacctt
     2941 caaccgtccc gaggtgaaca acgcctataa cggcgacctc atccagggcg tgctggacgc
     3001 catggacgat ctcggcgggc agcccggcct gcgcgtcgtg gtcctcaagg gcaacggcaa
     3061 acactttcag gccggcgccg atctcaagtg gatcaattcc gtgaggcccg gctcgcacga
     3121 agcgaatgtc gccgtctcgc gtgcgacggc cgaggccgtc gatcgtctca atcggctgcc
     3181 ggtgccagtc gtcgcgctcg tgcagggagg ctgcttcggc ggcggcacgg gcatcgtcgc
     3241 agcctgcgac gtggtgatcg cggccgacaa cgcgctcttc tcgatcgccg aggtgcgctg
     3301 gggcctgcac gccggcatca tcataccgca gctcaacgac gcgatgggcg tgcgccaggt
     3361 gcgccgctac gcgctgaccg gcgagcgctt cggggcggat gaggcgcgcc gtctcggcct
     3421 cgtccatgag gtggtggcgc tcgccgatct cgaggcgacg ggnnnnnnnn nncgtccgcc
     3481 agatcctcga gaactcgccg gacgccatcg ccaagacgaa gtcacatatt ctcgagagcg
     3541 cctggggcgg gttcgggcag acgacgttca acgagctcgt cgacagtcat gccaaggcgc
     3601 gccagtcgcc ggctgccgcc gaaggcctcg cctcgttcgc agagaagcgc tcagcgaaat
     3661 gggcgccgaa gccatgaggc gtggcgcaga gcgcatcttc gtggcactcg acgtgccgac
     3721 ggtcgaagcg gcgcgggctc tcgtggacac gctcggcgac agcgtcggcg gtctgaaaat
     3781 aggcctcgag ctcgtgatgg gcggcgggct gaagctcgcc gaggagctcg cgcgcgaggg
     3841 tcgccgcgtg ttcctcgaca tgaagctgct cgacattccg aacacggtcg aaggtgccgc
     3901 ggccaatgcg gccgaggccg gtgttgccta cctgaccgtt cacggcaccg atcgaaagac
     3961 gctcgatgcg gctgttaaag ggcgaggcaa ctcgaagacc aagctgctcg ccatcacggt
     4021 tctgaccagc ctcggctccg ccgatctccg cgagcagggc agcacgatgg cggtcgagga
     4081 gctggtcgtc caccgcgcgc ggctcgccaa cgcggccggc ttcgacggtg tcgtcgcctc
     4141 accgctggag atcgcggcga tccgggcggc ggtcggacag agcctcgaga tcgtgacgcc
     4201 gggcgtaagg cccgccggcg ctgccagggg cgatcagacg cgcgtcatga cgccagcaga
     4261 ggcgatccgc gcaggcgcca cggcgctcgt cgtcggccgt ccgatcacgg cggtcgccga
     4321 tccacgggcc gcagcgctcg ccatcggcgc cgagatcgag gcggcactcg gctgagttct
     4381 tcggaccgac gaattgcccg cggcgagcgg ctcgattaaa cttccgccgg tcgacaaaaa
     4441 gggattgcgg cccatgacca gctaccggct ccactgcttc gcgctgtcgg gaagcgccta
     4501 caaggtggcg ctctatctga actgcgcagg cctcaagtgg gaggcggtgc cggtcgattt
     4561 cctcaaaggc cagacgcggg atcccaaatg gcgcgacgag atcaacgaca tgggcgaggc
     4621 gcccgtgctc gaggccgacg gcaagcgcta ttcgcagtcg ggcgccattc tctcctggct
     4681 cgccgagacg acggtcaagt tcgtgccgaa cggggcggat gagcgctacg atgcgctgcg
     4741 ctggatcctg ttcgacaacc acaagttcac gagctatttc gcgacgcacc gcttcttgaa
     4801 ttcgatcgcg ccgcaggctc cggaccccgc cgtgcttggc ttcctgaaga cgcgcgttga
     4861 cgctgccttc gccatcgtcg agaagcatct cgcgacgcgg aagttcatgc tcggcgccaa
     4921 gccgacgatc gccgacttct cgctgatcgg gtacatgtac tacccgagcg aggaaacggg
     4981 ctacgatctc gccaagtcgc atccggcgat ctatgcctgg acacagagga tgcgattcct
     5041 gccgggttgg aaggggccgt acgagctgat gcccggtgtc cggccggaac tgcgcaaggg
     5101 ctgacgcggc ctaacggaca ttcgctgatg gcgccgtggt cagctgaccc gttccgccgc
     5161 gatggggcgt gcgacgagat ccgagcggtg cgccgagatg atccgggaga tctgatcggg
     5221 cgaggcatcg aggcggttcg tgtcgatgac gagctggctc gtgccgttgc gccgccggcg
     5281 ccgcgtgccg acgacgacga gtggcccata ggtctcgagc gagttcacat cgttccaaga
     5341 gagccgctcg gcgccgagca gcccgaaggt cgagatgccg ttcgcgtcga ccgtgatctc
     5401 gacggcgggg ctcagccacc gcgccagctc cacgatgccg acgaggaaca ggatcaccgc
     5461 gaaggccgcg agcaccgcgc cgacgatcca cgacggcggc gccacgaaga tgccgacgcg
     5521 ctgcggcagc tcgagcaggt tcatggcgag cgtgccgacg gtgaggaacg cgaacgccat
     5581 gggtagtgcc acgaggaagc cccagggcga tgatttcgag cgggcgatca aggcggtagc
     5641 tcccgagtat gcatgacagg tctgcgcgga cctgcctcaa ccgttagcag actgggtcaa
     5701 gccgtcagct ggcggcacgg gaattcctta atggcacttg gtgtcgcggc gaaggccgcc
     5761 gataccgcaa cgaatgcgag cgccgacggc cgtaccaacc tcatccctgg acggagaagc
     5821 cgccgtccac ggggattgcc gcgcccgtga tgaagtccga ggcatggctc gagaggaaga
     5881 tcgcgatccc ggagaaatca ttcggtccgc cccagcgggc cgccggcgtg cgtttcagca
     5941 cgctctcgtg cagcgtcggg atcacctcgc gcgcggtttt ggtgaggtcg gtgtcgatcc
     6001 agcccggcag gatggcgttc acctggatgt tgtccttggc ccaggccgtg gcgagtgcct
     6061 tggtcatctg cacgatgccg cccttggatg ccgcgtaggg cgtcgcgaac ggcgcgccga
     6121 agatcgacat catcgagccg atgttgatga tcttgccacc accggccttc ttgagatgcg
     6181 ggtgggcggc gtgcgagcag aagaaagcgc cgtcgagatt gatggcgatc acctcgcgcc
     6241 attcggcggg cgagtaggtc tcgggctgct tgcggttatt gatgccggca ttgttgatga
     6301 ggatgtcgag ccggccgaag gtcttcaccg tctcggcgat catcgccttg cacgaggcgt
     6361 cgttggcaac atcgacctcg acgaccgcgg tcttcacgcc gagctttgcg agctcgtctg
     6421 ccgcgccctt cgatttcgcg gccttgcggc cggcaatcat gatgttggcg ccggccttcg
     6481 cgaggccgcg cgccatgccg agaccgatgc cgccgttgcc gcccgtgacg acggcgacgc
     6541 ggcccttgag atcgaagagg gacatgcgac tttttcctcc ggtgtttccc cgccaacggg
     6601 taggaccatt ggcccgacgg atcaagcgcg cgctcatcct gtgcggtcag acctgcgcga
     6661 cccgaagccg cgccgcgaca cgctcacgtc ccatgagcgg catgagcgcg cggagctccg
     6721 ggcccttttc ctcaccggtc aaggcgatac gcagcgggtg gaagagcgcg cggcccgagg
     6781 cgccggtcgc ggccttgatc gcggcggtgt aggcggacca tccggccggc gaccaggtgt
     6841 ccgcgtccca cggcgcggcc ggcagtcggt cggctgcggc tttcaggacg tcggggctct
     6901 cgacggtcgg tgcgatcggg ccatacacga tgtcgtgcca gcgcttcgcg tcggcgagca
     6961 cggcaagatt gccacgaacg gtaagccaga acggcgcgcc cccgctgacg ccgagggcgg
     7021 cgaggcgcgc gctgacccgc tcgaacggca gcatgtggag caagcgcgca ttgagcgcgt
     7081 gcaggtcgtt ctcatcgaag cggccgggcg cccgcgaaat cttcccgagg tcgaaactct
     7141 tagccagatc gtcgagcgtc gcgcgcggct cgatggcatc cgaggtgccg acgagggctg
     7201 cgtggcagac cacggcttcc ggctcgagcc cggacttgcg caggccttcg atcgagagcg
     7261 cgccgagccg cttggagagc gcgctgccgt ccgcctggac gagaaggctg tgatgggcga
     7321 aggccggcgg ccgcgcgccg agcgcctcga agagctgaat ctgcacgccc gtgttggtga
     7381 cgtggtcctc accgcggatg atgtgcgtga tcgccgtctc gatatcgtcg acgacgctcg
     7441 tgaacgtata gaggaacgtg ccgtcctcgc gcacgagcac ggggtcggac agcgatcccg
     7501 catcgacggt ctgatgcccg cggatgaggt cgtcccaggc aaccggcgtc ggacgcggcg
     7561 cgagatcgtc cgggccgctc gaattggtta ggcggaagcg ccagtgcggc cggcggccct
     7621 ccgccgcaag cttcgccgtc tcggcgcccg tgaggcgcaa cgcggcgcga tcataaatcg
     7681 gcggcaggcc gcgcgcgagc tggcgcttgc gccgccggtc gagctcgtcg gcggtctcgt
     7741 agcagggata gaggcggccg gcggctttga ggcgctcggc ggcggcggcg tagcgctcga
     7801 gcctgtccga ctgccgcagg aacgcatccc agctgaggcc gagccagcgc atgtcctcga
     7861 cgatgccatc ggcgaatgcc gccgtcgagc gcccctgatc cgtatcatca aggcgcagca
     7921 ggaacgtgcc gccgtgccgc cgctggaaaa gccagttcag cacggccggg cggatgttgc
     7981 cgacgtggag cctgccggtc gggctcggcg cgaagcggac tttgacggac atggggctcc
     8041 agtcggctgg tgtgtgccgc cacagttact ccgggccggc cgcatgcacc agcctcagta
     8101 gctcttcggg agcccgagcg ccttttcggc gaggaaggag aggacgagct cgcggctcac
     8161 gggtgcgatg cgcgcgatca acacctcgcg gagataccgc tcgacgtgga actccttcga
     8221 gtagcccatg ccgccgtggg agaggatcgc ggtctcgcag gcctcgaacc cggcctcgcc
     8281 cgcgagatac ttgcccgcgt tcgcctcgat gccgcagggg aggcccttgt cgtagagctc
     8341 agccgcgcgg aagatcatca ggttcgcggc ctcgaggttc gcccagcagc gcgcgagcgg
     8401 atgctggatg ccctggttct cgccgatcgg ccgtccgaag acgacgcggt ccttcgcgta
     8461 ctgcgcggcc ttgcggatcg cggcccggcc gatgccgacg gcttccgagg cgatcaggat
     8521 gcgctccgga ttgagcccgt gcaggatgta ttcgaagccg cggccctcct cgccgatgcg
     8581 atcctcgatc ggaaccggca gcccgtcgat gaacagctgg ttgctgtcga cggccttgcg
     8641 gcccatcttg tcgatctcgc gcacctcgac gtatttgcgg tcgagatcgg tatagaagag
     8701 cgtgaggcct tcggtcggcc gcttcacctc ttcggcgggt gtcgtgcggg tcaggatcag
     8761 caccttgttc gcgacctgcg ccgtggaaat ccagatcttc tgcccatcga tgcgatagcg
     8821 gttgccgtcg cgtcgcgcgc gcgtcttgag cttggtcgtg tcgaggccga cgttcggctc
     8881 ggtgacggcg aagcaggatt tgtcggtgcc cttgatcagc ggcgggagga agcgcttctt
     8941 ctgctcctcg ctgccgaaca cgacgaccgg gttgagacca aagatgttca tgtgcacggc
     9001 cgaagcgccg gagaggcagg cgcccgactc ggcaatggtc tgcatcatca gcgccgcttc
     9061 cgtgatgccg agaccggcac cgccataggc ttccggcatg gcgatgccga gccatccggc
     9121 gtccgcgagc gctgcatgga agtcgtgcgg gaagccgccc ttgcgctcct tctcgagcca
     9181 gtaggcatca tcgaagcggg cgcagatctt ctggatggcg tcgcgaattt cgcgctgctc
     9241 gttcgtcatc agggcttcgt gcatggtcgc tctcgttatg cagttcgact gtcaagaaag
     9301 gccgggtcct ggtggcacct gcgcatctgg cgcgcgtcgt cagaaatgcc ccgatcgggc
     9361 gcgggcggaa gcccgttcgg caggcgaggc tgccgcatca cgactttgcc gtgccgacga
     9421 caccgcgggc gatcaggtcc ttgatgcgct ccggcgcgaa gcccgcggcc ccgagcacgg
     9481 cgtccgtatc tgtgccgacg ccgcgcgagg cagcacctgc tttggctgct gtcctcgaaa
     9541 agcgcggcgc gggtgctgcc tgcatgacgc cgtcgacgcg caagaacgtc ttccgctcgc
     9601 ggttgtgcgg atgagcttcc acttctgcca tcgtgagcac gggcgcgaag caggcgtcgc
     9661 tcccttcgaa cacctggcac cacgcatcgc gtgacttcgt cttgaaggcg gcggcgaagc
     9721 gctctcgcag catcggccag ccggacttgt cccactgctt cggcagcttc tcgccagcga
     9781 gcccgaggcg ggcaaggagg tcctcgtaga agcgaccctc gatcgagccg atcgacaccc
     9841 actttccgtc cgaggtctcg tagacctcgt accagggcgc gcccgtatcg agaatgttga
     9901 cgccgcgctc gtccttccag gtgccttgcg agtagaggcc gtgtatgccg gccatgagga
     9961 gcgcggcgcc atcgaccatg gcggcatcga caacctgacc ttttccggag cgcgaagctt
    10021 cgagcagggc cgcgagcacg ccgacggcga gcagcatgcc gccgccgccg aaatcgccga
    10081 cgaggttcag cggcatggtc ggcggtgctc ccttgcggcc gacggcgccg agcacgcccg
    10141 cgagcgcgat gtaatttatg tcgtggcccg cgcgcggcgc cattgggcct gtctggccga
    10201 atcccgatac gcggatcatg atgagccgcg gattgatctt cgcgaggtcc tcgtagccgc
    10261 agccccattt ctccatggtg ccgggacgga aattctcgag caggaagtcg gccttc
//
LOCUS       DAORNH010000154        10239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4855_length_10239_cov_2.304497, whole genome shotgun sequence.
ACCESSION   DAORNH010000154 DAORNH010000000
VERSION     DAORNH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_10239_cov_2.304497"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK264_15145"
     CDS             <1..349
                     /locus_tag="PK264_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77243.1"
                     /translation="THLGYRMRICCLGGDIEGALEAMPRASDVPAFPGWKCALLAHAD
                     RLEEARLELKRFYHLAAANWAGVDAWSETGTVRWFLHAFPLRRRGDWERLRDGLSLAG
                     APVHGLRHRFWTH"
     assembly_gap    494..522
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<523..1342)
                     /locus_tag="PK264_15150"
     CDS             complement(<523..1342)
                     /locus_tag="PK264_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD77244.1"
                     /translation="MKAAVVTDKGVEVREVPSPKPGPDQVLVRVRAAGLNRADLIVAS
                     GRAHGAVGGAGTIMGLEFSGEVAEVGANVSGITAGDRVMCSGSGGYAEYAVADKGRVS
                     PIPANNMGWVEAATLPVALQTMHNAVVTAGRLAPGETVLIQGASSGVGLMAMQIAKLK
                     GARLVIGTSTDAGRRGRLGEYGADVVLDSRDPGWADAAIAATGGKGLDLIVDQVSAGV
                     ANANMKAASVRGRIVNVGRLGGFTGEFDFDLHAMKRIDYIGVTFRTRSVEEVREI"
     gene            complement(1855..2205)
                     /locus_tag="PK264_15155"
     CDS             complement(1855..2205)
                     /locus_tag="PK264_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77245.1"
                     /translation="MNAGLEKHRQRILVPIRVVGEALGLVIGEHDTFQVGGRHVAHGD
                     IDRRSELVAWQSGRLEEHRTQLIVARRRLERPRRHEADELRLTGPGAMLGEYPRDVGQ
                     DAHTEALLGETRRF"
     gene            2342..3130
                     /locus_tag="PK264_15160"
     CDS             2342..3130
                     /locus_tag="PK264_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77246.1"
                     /translation="MAAIREPLDLLALDVPGSAVDKIVELSVGYPYLIQFFAKEMVDA
                     VLHAGAGALEGFPSEAVWRRLDSGIFAARWGRTTDKQREALKVMARLPAETAEFTPHD
                     VADGSAEFGIGAMTNAQAGQIMQGLVERGLIYRTRRGKYAFALPMSRVMILRRMLDDD
                     DQGLPGFADLSAPAEIVAHQPASDELEIEERPGPAIYASGAAVDDSEPAPMSDELVAA
                     FREVMRTVPVRPQREVLRGSPRADASAMAAPAASSFWSFRGMRS"
     assembly_gap    3368..3506
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3507..4133)
                     /locus_tag="PK264_15165"
     CDS             complement(<3507..4133)
                     /locus_tag="PK264_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77247.1"
                     /translation="MARNRLLAIVAGVAIVVAACPAMAQDDGHSPETVRFASADGKTD
                     LVGYVFRRPDAKAPMPGVVMMHGRGGPYSERAKGTFDATTLSLRHKAWGRLWAEAGYV
                     AILVDGFGPRGYPKGFPRGSYDSRPAELNEVTIRPLDAYGALAYLRSRGDVIADRIGL
                     HGWSNGGSAALSSIADTAPGIASPTPATGFRAALVFYPACGLKGHFDER"
     gene            4379..>6193
                     /gene="leuS"
                     /locus_tag="PK264_15170"
     CDS             4379..>6193
                     /gene="leuS"
                     /locus_tag="PK264_15170"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRD77248.1"
                     /translation="MASERYNAPEREKHWQKVWDAKGAFRAREDGGRPKYYVMEMFPY
                     PSGRLHVGHSRNYTIGDAVARFKRAQGFNVLHPMGFDAFGLPAENAALERKVHPASWT
                     RENIDFMRRQLKSLGLAIDWEREVVTCDPSYYCHQQKLFLDFYAKGLAYRKPSKVNWD
                     PVEQSVLANEQVEDGRGWRSGALIEQRELTQWFFKTTAFSDELLKALDTLEQWPEKVR
                     RMQANWIGRSEGLMVRWKLGKGAPKGHGELEVYTTRPDTLFGASFMAIAPDHPLAKAL
                     AEKDNKLAEFAEECRRMGTSVADLETAEKKGYDTGLKAIHPFDASWEVPVYVANFILM
                     DYGTGAIFGCPSGDQRDIEFARKYKLPVLPVVVPKAAAEQADVLTAIKHKGIAYAEDG
                     VMINSRFLDGMDTASAFNEVASRLEAERLGGKPVGKRTVNYRLRDWLLSRQRYWGCPI
                     PIIHCDDCGVVPVPDKDLPVQLPDDATFDVPGNPLDRHPTWKNVPCPTCAKPARRDTD
                     TMDTFVDSSWYFVRYTAPDAKTPVDPNRAGYWLAVDQYIGGIEHAILHLLYSRWFVRA
                     MSACGYLQLDEPFTRLFTQGMVVHETYRRLDGNKPGEWL"
     assembly_gap    6194..6410
                     /estimated_length=217
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6411..>6763
                     /locus_tag="PK264_15175"
     CDS             <6411..>6763
                     /locus_tag="PK264_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77249.1"
                     /translation="GARRFVQRIWRMIDDVAGKVPKGAKAAGEATPESLALRRAAHRA
                     LHAVGQGIEAFRFNVAVAQIYELSNVLSASAHKGGAALGPALREAAELLVTMIGPMMP
                     PAAIRLTPSRITSKAS"
     assembly_gap    6764..6773
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6774..7036
                     /locus_tag="PK264_15180"
     CDS             <6774..7036
                     /locus_tag="PK264_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRD77250.1"
                     /translation="ARLGYNTLLIDQPWPKADPALLVDDSVTIAVQINGKRRAEVTVA
                     RAASQREIEATVLALDSVQKALEGKTPRKVIIVPQRIVNVVV"
     gene            7023..7586
                     /gene="lptE"
                     /locus_tag="PK264_15185"
     CDS             7023..7586
                     /gene="lptE"
                     /locus_tag="PK264_15185"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRD77251.1"
                     /translation="MSWSDHKLKRARRPVGRLSALVLVPAMLIGTLVGGCGGGGFQPL
                     YGPTMSGVALDKRMAEVDVLQVPGRVGQRIRNELIFQTTGGADPVPHAYRLEIAIRES
                     ILSTLVRSTGDSAGQIYSLDAHFRLISIKDKKVLVQGVSHARASFERYDSIFSNVRAR
                     EDAENRAARTAASEIKMRIAAFLSGNT"
     gene            7605..>8408
                     /gene="holA"
                     /locus_tag="PK264_15190"
     CDS             7605..>8408
                     /gene="holA"
                     /locus_tag="PK264_15190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRD77252.1"
                     /translation="MVAVKAGQAEAFVKAADPKLQAFLLYGPDEGLVSERSQSLARRI
                     AERENPPGEIVRLGDADLEEGSERLEVELLTIPMFGGRKIVRATAGRRINAALLKPLV
                     EGGRLAGVLIVEAGNLRPDESLRATFEKGSLAAAIACFPDEARDIDALIREVLGEAKM
                     EIAPDARSHLASRLGADRVLSRSEIEKLALYAQGRGRIEVDDVDAVVGDASELALDKI
                     VNAAVSGRVADALSEFDRAVAAGENPQGIVMAVQRHVQRLHRIRATLDGG"
     assembly_gap    8409..8436
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8437..8641
                     /locus_tag="PK264_15195"
     CDS             <8437..8641
                     /locus_tag="PK264_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRD77253.1"
                     /translation="LRSIRPPLHFKQRDAVAAQLRQWTGDRLLAAAVRIGEGLKAARL
                     GGTLESAHAERLLLEIGRLATRR"
     gene            complement(8661..>9088)
                     /locus_tag="PK264_15200"
     CDS             complement(8661..>9088)
                     /locus_tag="PK264_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRD77254.1"
                     /translation="TMMPMGEIARLAALQGSEINDAKKILATEVTAMLHGRDKAEAAA
                     ATAAKTFEEGALGEGLPSIDVPKAELEAGIGVIALFVRAGLVKSNSDARRQIQGGGLR
                     VNDVAVSDDKAMVSTRDLSAGGAIKLSFGRKKHVLVKVG"
     assembly_gap    9089..9116
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9117..9929)
                     /gene="tyrS"
                     /locus_tag="PK264_15205"
     CDS             complement(<9117..9929)
                     /gene="tyrS"
                     /locus_tag="PK264_15205"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRD77255.1"
                     /translation="MAKYKSDFLNALDERGYIHQCSDFAGLDQLAAAGKAIGYIGFDC
                     TAPSFHVGNLIVLMMLRWMQETGNKPIVLMGGGTTRVGDPSGKDESRKILPIEQIEAN
                     KASMQRACAKLMRFGTGHSDAVMLDNAEWLTKLNWIEMLRDIGRHFSVNRMMALESVK
                     SRLDREQEMSFIEFNYQILQAYDFTVLAKSHGCNLQMGASDQWGNIVTGIDLGRRLGT
                     HQLYALTTPLLTTASGAKMGKSVAGAVWLNEEMLGAYEFWQFWRNVEDADVGR"
     gene            10026..>10239
                     /locus_tag="PK264_15210"
     CDS             10026..>10239
                     /locus_tag="PK264_15210"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HRD77256.1"
                     /translation="MGKIYRAIGLMSGTSLDGIDVAMIETDGEARVRRLGGVTVPYPD
                     AFRARLRQALGEAVAVQNRLERPGILA"
BASE COUNT         1622 a         3159 c         3361 g         1646 t
ORIGIN      
        1 cacgcacttg ggctatcgga tgcgcatctg ctgcctcggc ggcgacatcg agggcgcgct
       61 cgaggccatg ccgcgggcct cggatgtgcc ggcgtttccc ggctggaagt gcgcgctcct
      121 tgcccatgcc gatcggctcg aggaggcgcg tctcgagctg aagcgcttct atcatctggc
      181 ggcggccaac tgggcaggcg tagacgcgtg gagcgaaacg ggcaccgtgc gctggttcct
      241 gcacgcgttt ccgctgcgcc gccgcggaga ctgggagcgg ctgcgcgacg ggctcagtct
      301 cgcgggagcc ccggtgcacg ggctccgcca ccgcttctgg actcactaga tccgggtatc
      361 agacccatgg gtctgatacc atcacacctc gagcacgatc ttgccgaagt gcttgttggc
      421 cttcatgtgg gcgagggcgg cggcggcctc cgcgagcggg aaggtgcggt cgagcggcag
      481 gcggagcttg ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgatctcg cggacttctt
      541 ctaccgagcg cgtgcggaag gtgacaccga tgtagtcgat gcgcttcatc gcgtggagat
      601 cgaagtcgaa ctcgccggtg aagccgccga gccgtccaac gttgacgatg cgtccacgca
      661 cggaggcggc cttcatgttg gcgttggcga cgccggccga gacctgatcg acgatgagat
      721 cgagcccctt gccgccggta gcagcgatgg cggcatccgc ccagccggga tcgcggctgt
      781 cgagtacgac gtcggctcca tattcgccga ggcgcccgcg tcggcctgca tcggtcgatg
      841 tgccgatgac gaggcgagcg cccttgagct tggcgatctg catcgccatg agaccgacgc
      901 ccgagctcgc gccctggatg agcacggtct cgccgggtgc gaggcggccg gccgtcacca
      961 ccgcgttgtg catggtctgg agggcgacgg gcagcgttgc ggcctcgacc cagcccatgt
     1021 tgttcgccgg gatcggcgag acgcggccct tgtcggcgac cgcatactcg gcatagcccc
     1081 cgctgccgga gcacatgaca cggtcgccgg ccgtgatacc ggagacattg gcgccgacct
     1141 cggcgacttc gcccgaaaac tccagtccca tgatggtccc ggcgccgccg acggcgccgt
     1201 gggcacgacc ggaggcgacg atcaggtcgg cgcgattgag acccgctgcc cgtacgcgga
     1261 cgagcacctg gtcggggccg ggcttggggg acggaacttc gcggacttca acacctttgt
     1321 cagtgacgac ggcagctttc atgacccagc tcctttggtg tgcatgcagg gagcgtgaac
     1381 gatcagcggc taagaggcaa cgagcgttga aactatttct ccatgcgttg ccggatcgtc
     1441 cgtgagtaat gtttgtggag atactgtgga taactttccg gttcacgcag aggttgtgtc
     1501 ggatgtgctc gcgttcgccc taactgcggt gcggcgtgtg agcggtcgtg ctcgcgccgg
     1561 atcctgtctc gagttcagtg gtgtgtggga gtggcgggac acgtgagtgc tcagcggaat
     1621 gtgcgtcggt cgcagcaagt caagaatccc tatcgtccgg gcgcgggcca tgcgcctccg
     1681 tacctcgcag ggcgaggctt cgagcaggcc gagttccggc gcctgcttgc ccaggacacc
     1741 atcaccgaga acgtgctgat caccggcctg cgcggcatgg gcaagaccgt gctgctcgag
     1801 accttcgccg ggctcgcccg tgacacgcgc tggctctggg tcggcaatga cctgtcagaa
     1861 tcggcgggtc tctccgagga gcgcctcggt gtgcgcatcc tgaccgacat cgcgcgggta
     1921 ctcgccgagc atcgcaccgg gtccggtgag gcgcagctcg tcggcttcgt gccgacgggg
     1981 ccgctcgagc cgccgccgag ctacgatcag ttgtgtgcgg tgttcctcaa ggcgcccgga
     2041 ctgccaagcg acaagctccg agcggcgatc gatatcgcca tgcgctacgt ggcgaccacc
     2101 gacctgaaag gtatcgtgct cgcctatgac gaggcccagt gcctcgccga ccacgcggat
     2161 cggcacgagt atccgctgtc gatgcttctc gagacctgca ttcatctcca gaagcgctat
     2221 ccggaccagg gatttatgct ggtgctgacg ggcttgccaa cgctgctcga cagcctgacg
     2281 atgagccgga cctactcgga gcgcatgttc aacgtcatgg cgctcgaccg gctcggatac
     2341 cttggcggcg atccgcgagc cgctcgacct gctggcactc gatgtgccgg ggagtgccgt
     2401 cgacaagatc gtggagctct cggtcggcta tccctatctc atccagtttt tcgccaagga
     2461 gatggtcgac gcagtccttc atgcgggagc aggcgcgctc gaaggcttcc cgtcggaggc
     2521 tgtctggcgc cgcctcgaca gcggcatctt cgcggcgcgc tggggccgca ccacagacaa
     2581 gcagcgcgag gctctgaagg tgatggcccg gctcccggcc gaaactgcgg aattcacgcc
     2641 gcacgacgtc gcggatggct cggccgagtt cggcatcggc gcgatgacca acgcgcaggc
     2701 cgggcagatc atgcagggcc tcgtcgagcg cggcctcatc taccgcacac gtcgcggcaa
     2761 gtacgcgttt gcgctgccga tgtcgcgcgt gatgatcctc cggcgcatgc ttgatgacga
     2821 tgatcaaggg ttgccggggt tcgccgacct cagcgcgccg gccgagatcg ttgcccacca
     2881 gcccgcttcc gacgagctcg agatcgagga aagaccaggt ccggcgatct atgcatccgg
     2941 agcggccgtg gacgacagcg agccggcgcc gatgagcgac gaactggtcg ccgcattccg
     3001 cgaggtgatg cgcacggtgc cggtccgccc ccagcgcgag gtgctgcgcg gttcgccccg
     3061 ggccgacgca tcggccatgg ccgcgcccgc cgcgtcgtcg ttctggagct ttcgagggat
     3121 gcgctcctga acggcgagcg cgactgcgga attcctggtg cttcggcggc gcaagctgcg
     3181 gagtagcgaa cggcggcgga gcagcggggc cgccgccgtg cgcaattcat tcgagcaggc
     3241 gagctgggtc accggcctca tcggcggccg gccgtgtctg gatggacgtg ggatgcgaag
     3301 aatgcgagcg aacggcgcac ggcatcctcg gtcgcgctcg cattggcatc gaggctctgg
     3361 cgagcgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnngcgc tcgtcgaagt gtcctttgag accgcacgcc
     3541 ggataaaaca cgagcgccgc ccgaaatccc gtcgcgggcg tcggcgaggc gatgccgggc
     3601 gcggtgtcgg cgattgagga aagtgcagca ctgccgccgt tcgaccagcc gtggaggccg
     3661 atgcggtcgg cgatcacatc gccgcgcgag cgcagatacg caagggcgcc ataggcatcg
     3721 agcggacgga tcgtcacctc gttgagttcg gcggggcggc tgtcgtagct gccgcggggg
     3781 aagcctttcg ggtagccacg aggcccgaag ccatccacga gaatggccac ataccccgcc
     3841 tcggcccaga ggcggcccca ggccttgtgg cggagcgaga gcgtggttgc gtcgaaggtc
     3901 cccttggcgc gctccgagta gggtccgcct cggccatgca tcatcacgac accgggcatc
     3961 ggcgctttgg cgtcggggcg gcgaaagacg taaccgacga gatcggtttt tccatctgcg
     4021 ctcgcgaagc gcacggtctc tggggaatgg ccatcatcct gggccatggc cgggcatgcc
     4081 gcgacaacga tggcgacgcc ggcgacgatc gcgagcagac gattgcgggc catggccgga
     4141 tgctccatgc gacggcatcg attgataata gcctgacaca atcctacctg gcgactaatg
     4201 ctggcgatac gggggcgcaa ccgtttgctc gcatgtacca tttgcgccca ggactgaatt
     4261 cggccgaaac cagtcactgc aggcgtcgtc agggagcgac accccgctgc cggcttgacc
     4321 ccgatgcccc tttcgggctc aaaggcgccg agacattcga ttgatccgag agcccgtcat
     4381 ggcgagcgaa cgctacaacg cgcctgagcg cgagaagcac tggcagaagg tgtgggacgc
     4441 gaagggcgcc ttccgcgccc gcgaagatgg cggccggccc aagtactacg tcatggaaat
     4501 gttcccttac ccgtcagggc ggctccacgt cgggcactcg cgcaactata cgatcgggga
     4561 cgcagtcgct cgcttcaagc gcgcccaggg cttcaatgtg ctgcatccga tggggttcga
     4621 cgcgttcggg ctgccagccg agaacgcagc gctcgaaagg aaggtccacc cggcgagctg
     4681 gacgcgcgag aatatcgact tcatgcgccg ccagctcaag tcgctgggcc tcgctatcga
     4741 ttgggagcgc gaggtcgtca cctgcgatcc ctcctactat tgccaccagc agaagctgtt
     4801 cctcgatttc tacgcgaagg gcctcgccta ccggaagccg agcaaggtca actgggaccc
     4861 ggtcgaacag tcggtgctcg ccaacgagca ggtggaggac ggccgcggct ggcgttccgg
     4921 cgcgctcatc gaacagcgcg agctcacgca gtggttcttc aagacgacgg cgttctcgga
     4981 cgaactcttg aaggccctcg acacgctcga gcagtggccc gagaaagtgc gccgcatgca
     5041 ggcgaactgg atcggccgtt cggaaggcct gatggtgcgc tggaagctcg gcaagggcgc
     5101 gcccaaggga catggcgagc tcgaggtcta tacgacgcgg ccggacacgc tgttcggcgc
     5161 ctcgttcatg gcgatcgcgc ccgatcatcc gctcgccaag gcgctcgccg agaaggacaa
     5221 caaactcgcg gagttcgccg aggagtgccg ccgcatgggc acctccgtcg ccgatctcga
     5281 gaccgcagag aagaagggct acgacaccgg tctcaaggcc atccacccct tcgatgcgtc
     5341 gtgggaggtg ccggtctacg tcgcgaactt catcctgatg gactacggca cgggcgcgat
     5401 cttcggctgt ccgtcgggcg accagcgcga tatcgagttc gcgcggaaat acaagctgcc
     5461 cgttctgccg gtcgtcgtac cgaaggctgc ggccgagcag gcggacgtgc tgacggcaat
     5521 taagcacaag gggatcgcct acgccgagga cggcgtgatg atcaattcgc gcttcctcga
     5581 cggaatggac acggcgagcg ccttcaacga agtggcgagc cggctggagg cggagcggct
     5641 cggcgggaaa cccgttggca agcgcacggt caactatcgg ctccgtgact ggctgctgtc
     5701 gcgccagcgg tattggggct gcccgatccc gatcatccat tgcgacgatt gcggcgtcgt
     5761 tccggtgccc gacaaggatc taccggtgca attgccggac gatgcaacct tcgacgtgcc
     5821 gggcaatccg ctcgaccggc atccgacttg gaagaacgtg ccatgcccga cgtgcgcaaa
     5881 gcccgcgcgc cgcgacacgg acacgatgga caccttcgtc gacagctcct ggtactttgt
     5941 gcgctatacg gcgccggatg ccaagacgcc cgtcgatccg aaccgggccg gctactggct
     6001 cgcggtcgac caatacatcg gcggcatcga gcatgcgatc ctgcatctcc tctattcgcg
     6061 ttggttcgtg cgggcgatga gcgcctgcgg ctacctgcaa ctcgacgagc cattcacgcg
     6121 gctcttcacg caaggcatgg tggtgcacga gacgtaccgc cggctcgacg gcaacaagcc
     6181 gggtgaatgg ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcgcacgcc
     6421 gcttcgtgca gcgcatctgg cgcatgatcg acgacgttgc cggaaaggtc ccgaagggcg
     6481 ccaaggcggc cggcgaggcg acgcccgagt cgctcgcgct gcggcgggcg gcgcaccgcg
     6541 cgctgcacgc tgtcgggcag gggatcgagg ccttccgctt caatgtcgcg gttgcgcaaa
     6601 tctatgagct gagcaatgtt ctttcggcgt ctgcgcacaa gggcggcgcg gcgctgggcc
     6661 cggccctcag ggaggcggcc gagttgcttg tcactatgat cgggccgatg atgccgcccg
     6721 ccgcgatcag gctaacaccc tcaaggatca cgtcaaaagc atcnnnnnnn nnngggcgcg
     6781 gctcggatac aacactctgc tcatcgatca gccctggccc aaggccgatc cggccttgct
     6841 cgttgacgac agcgtgacca tcgccgtaca aatcaacggc aagcggcgtg ccgaggtcac
     6901 ggttgcgcgg gcggcgtcgc agcgcgagat cgaggcgacg gtgctggctc tggacagcgt
     6961 tcaaaaggcg ctcgaaggca agacgccgag aaaagtcatc attgtcccgc agaggatcgt
     7021 gaatgtcgtg gtctgatcac aagctgaagc gcgcccggcg gccggttggt cgcctctctg
     7081 cgctcgtgct tgtgccggcc atgctgatcg gaaccctcgt cggcggctgc ggcggtggcg
     7141 gcttccagcc gctctatggg ccgaccatgt cgggcgtcgc gctcgacaag cgcatggccg
     7201 aggtggatgt cctgcaggtg cccggccgtg tcggccagcg catccgcaat gagctgatct
     7261 tccagaccac gggtggcgcc gatccggtgc cacacgccta ccggctcgaa attgcgatcc
     7321 gcgagtcgat cctctcgacg ctggtgcgct cgacaggcga ttcggcaggc cagatctaca
     7381 gcctcgacgc gcacttccgg ctcatctcca tcaaggacaa gaaggtgctc gtgcagggcg
     7441 tcagccacgc gcgggcaagc ttcgagcgct acgactcgat cttctcgaac gtgcgcgccc
     7501 gcgaggacgc cgagaaccgc gcggcccgca cggcggcgag cgagatcaag atgcggatcg
     7561 ccgccttcct gtccggcaat acctgagacg gccgagggcc agtgatggtg gcggtgaagg
     7621 ccggtcaggc ggaggccttc gtcaaggccg cggatcccaa gctccaagcc ttcctgctct
     7681 atggtcccga tgagggcctc gtctccgagc ggagccagag cctcgcgcgg cgcatcgccg
     7741 agcgcgaaaa cccgccaggc gagatcgtgc ggttaggtga tgccgatctc gaggagggct
     7801 ccgagcgcct cgaggtcgag ctgctgacga tccccatgtt cggcggccgc aagatcgtgc
     7861 gcgcgacggc ggggcggcgc atcaatgcgg cactgttgaa gccgcttgtc gaaggggggc
     7921 ggcttgccgg cgttctgatc gtcgaagcgg gaaacctgcg tccggatgaa tcgctccgcg
     7981 cgaccttcga gaaggggtcg cttgccgccg ccatcgcctg ctttcccgac gaggcgcgtg
     8041 acatcgatgc cctcatccgc gaggtgctcg gtgaggcgaa gatggagatc gcgcccgatg
     8101 cgcggagcca tctcgcgagc cggctcgggg ccgaccgggt cctctcccgc tccgagatcg
     8161 agaagctcgc gctctatgcg cagggccgcg ggcgcatcga ggtcgacgat gtcgacgccg
     8221 tggtcggcga tgcctcggag ctggcgctcg acaagatcgt caatgccgcc gtgtcggggc
     8281 gcgtcgccga tgcgctctcc gagttcgatc gcgcggtcgc ggcgggcgag aacccgcaag
     8341 gcatcgtcat ggccgtgcag cgccacgtgc agcggctgca ccgcatccgg gcgacgctcg
     8401 atggcgggnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg aggtcgatcc gcccgccgct
     8461 gcatttcaag cagcgcgatg ccgtcgcggc ccagctccgg cagtggactg gggatcgcct
     8521 gctggccgca gccgtgagga tcggggaggg cctcaaggcc gcgcgcctcg gcggcacgct
     8581 cgaaagcgcg cacgccgagc ggctactgct cgagatcggg cggctcgcga cgcggcggtg
     8641 agggcccgcc cgaaggccga tcagccaacc ttcaccagca cgtgcttctt gcgaccgaac
     8701 gagagcttga tggctccgcc ggcggagagg tccctggtcg agaccatcgc cttgtcgtcg
     8761 ctgacggcga cgtcgttgac gcgcagtccc ccgccctgga tctggcggcg ggcgtcgcta
     8821 ttcgacttga cgaggcctgc acgcacgaac agggcgatga cgccgatgcc ggcttcgagc
     8881 tcggccttcg gcacgtcgat gctcggcagt ccctcgccga gggcgccctc ctcgaaggtc
     8941 ttggcggccg tcgctgccgc ggcctcggcc ttgtcgcggc cgtgcagcat ggccgtcacc
     9001 tcggtcgcga ggatcttctt ggcgtcgttg atctcggagc cctggagagc cgccaaccgg
     9061 gcgatctcgc ccatcggcat catggtgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg
     9121 ccgacgtcgg catcctcgac gttgcgccag aactgccaga actcgtaagc gccgagcatc
     9181 tcctcgttga gccagacagc accggcgacg ctcttcccca tcttggcgcc cgaggcagtc
     9241 gtaaggagag gggtcgtcag tgcgtagagc tggtgcgtgc cgagccggcg accaaggtcg
     9301 atgccggtga cgatgttgcc ccactggtcc gaggcgccca tctgcaggtt gcaaccgtgg
     9361 ctcttcgcga gcaccgtgaa gtcataagcc tgcaggatct ggtagttgaa ctcgatgaag
     9421 ctcatctcct gctcgcgatc gaggcgcgac ttcaccgatt cgagcgccat catgcggttg
     9481 accgagaagt ggcggccgat gtcgcgcagc atctcgatcc agttgagctt cgtcagccac
     9541 tcggcgttgt cgagcatcac ggcatcagag tgacccgtac cgaagcgcat gagcttggcg
     9601 caggcgcgct gcatggatgc cttgttcgcc tcgatctgct cgatcggcag gatcttgcgc
     9661 gattcgtcct tgccggaggg gtcgccgacg cgcgtcgtgc cgccgcccat gagcacgatc
     9721 ggcttgttgc ccgtctcctg catccagcgc aacatcatga gcacgatgag gttgccgacg
     9781 tggaacgagg gcgccgtgca atcgaagccg atatagccga tcgccttgcc ggcggccgca
     9841 agctggtcga gcccggcgaa gtccgagcac tggtggatat agccgcgctc gtcgagggcg
     9901 ttgaggaagt cggatttgta tttggccatg atcgtggctc cgtattcttg gctctcgctg
     9961 gcgggcgcga agccctgtag tctttcattg cgcaagtcgg actggaccaa gcacaggacg
    10021 gcacgatggg caagatctac agggcgatcg gcctcatgag cggcacctcg ctcgacggca
    10081 tcgacgttgc catgatcgag acggacgggg aggcgcgcgt gcgccggctc ggtggcgtca
    10141 cggtccctta ccctgatgcg ttccgggcgc ggctgcgcca ggcgctcggc gaggccgtgg
    10201 cggtccagaa ccggctcgag cggccgggca tccttgccg
//
LOCUS       DAORNH010000155        10220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4869_length_10220_cov_2.448303, whole genome shotgun sequence.
ACCESSION   DAORNH010000155 DAORNH010000000
VERSION     DAORNH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10220
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_10220_cov_2.448303"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>206
                     /locus_tag="PK264_15215"
     CDS             <1..>206
                     /locus_tag="PK264_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77257.1"
                     /translation="STDEADVELATALNLDREIAFRSSLLNARLVRYRIPDNRPIAEV
                     LAAIATRAGVISASPEYIYRPVQG"
     assembly_gap    207..371
                     /estimated_length=165
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <372..1038
                     /locus_tag="PK264_15220"
     CDS             <372..1038
                     /locus_tag="PK264_15220"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRD77258.1"
                     /translation="PVPEAHGTSIAGILVARNQMKGIAPEARVVSVRAFSSAGAGRSP
                     EATTLSVIKGLEWAFIGGTRLYNLSFTGPRDPLLEQLLERAEAQGAVFVAAAGNAGPG
                     APAAYPAAYKTVVAVTAVDANDRVYARASRGPYVAIAAPGVDILAPTLASGYGMSSGT
                     SMAAAHVTGAIALVMEKRPDLKPAQIREVLLRAVRPGDAASARDIGAGILDAAKLIEV
                     VAR"
     gene            1120..1899
                     /locus_tag="PK264_15225"
     CDS             1120..1899
                     /locus_tag="PK264_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77259.1"
                     /translation="MRLEGKIGIVTAAASGMGRAGALRFAREGASVGVVDIDAKGVES
                     VVAEIRRAGGSAHAIVADLTRDEDSRRIVKDTAKAFGGLDFVWNHVGHPGPASFEGLD
                     LEEWDNAVALNLRTVLVTSEAAIPEMRARGAGAMLYTASTSGISGSQFSPVYSAVKHG
                     VVGLVRGLAKRYAKERIRINAICPGPIDTPMLRTFVARPDQQSTLGMDKEELVRKRAY
                     QTPMGRPGQPEEVANAALFLLSDEASFVTGACLTVDGGATC"
     gene            complement(2052..3200)
                     /locus_tag="PK264_15230"
     CDS             complement(2052..3200)
                     /locus_tag="PK264_15230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD77260.1"
                     /translation="MSAATASSTPPETSFDWRATLAELAPRLEATERRCDQTKDYVAD
                     NMALLREHGFFALAVPSELGGAGLLPAEVAEFLRTLARTSSATALTMSMHTHPVAFAA
                     WRWRHQKAPTDALLRRIAAERLQILTSGGSDWLPGSGIATKVEGGYKIDGRKVFASGA
                     PSANLFMTMAVEQTPEGPMVMHIAVPLTSPGISIVETWDTMGMRGTASHDVVFENVFV
                     PDAAVSVRRPSGAWHPIMHTISMVAFPLVYAVYTGIAEAACEIAIAAAKRKGTPSVDL
                     VGELQTEWTATRVAHESMVAFAAVAQPGEATTSEIFTYRSLIARSALRTVELALEVAG
                     GASYFRKQGLERLFRDIQGARFHPLRPAEQRKLAGRVALGLPVDEMRA"
     gene            3263..4321
                     /locus_tag="PK264_15235"
     CDS             3263..4321
                     /locus_tag="PK264_15235"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRD77261.1"
                     /translation="MTEKPGLPAETPDVLSDMLRAVRLTGAVFFSGRYTAPYGIRSTK
                     RFDPSMPAAHLRHISVFHLLTAGTCMFESASGSQRRLTAGDLLLIPFAGEHQFWEGDD
                     VRIVPVENTVRQGHHPGLWTSDHGGSGPETRLVCGLLESSEFLAMPLFRTLPEFVIAR
                     PGDFKAGELVVATVRDIMALVDAASPGADIMLGRMMELLFIEMLRRHASSLPQDSRGL
                     LAAMNDPVVGQALKLVHAEPARHWTVDSLARKTGASRTVLAERFNAFLGRPPIDYVIG
                     WRIQLAAERLRNGRDPIATIAADVGYESEAAFGRAFKRVTGISPGRWRNGAGDSPDLM
                     PLQFKTPLGPVADDAAVE"
     gene            complement(4362..>4855)
                     /locus_tag="PK264_15240"
     CDS             complement(4362..>4855)
                     /locus_tag="PK264_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77262.1"
                     /translation="QILVLQAQGIGKGDAILIWMIVGPAQAAARFAELVFAHKHSIYT
                     TALASAVLTTASFLAFLPFGVSITTAIVFSFAIGFGHGLFAIARNTLPLTLFGARDYG
                     SYMGLLMVPQNIVNATAPVIFAWLIARVSPMAAMWFAAAAAFAGFLAVLALISACRRP
                     VSA"
     assembly_gap    4856..4865
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4866..>5198)
                     /locus_tag="PK264_15245"
     CDS             complement(<4866..>5198)
                     /locus_tag="PK264_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77263.1"
                     /translation="PNARRAITGLMLLSGLTSTIFLPLISKLADLFGWRGTYIVFAIL
                     HAGICLPVHALIMHRAEASGDGRSPARSGAAAPAEAPLPPEHRRRALFMLATWSCTEG
                     LITWGLYL"
     assembly_gap    5199..5340
                     /estimated_length=142
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5341..5604)
                     /locus_tag="PK264_15250"
     CDS             complement(<5341..5604)
                     /locus_tag="PK264_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD77264.1"
                     /translation="MISAQSLAIVRRPPVIVAALGVTQIIGWGSIFSPLTVLGTPIGH
                     DIQAPREVVFAGITVMLLVSALFAPRIGKRVDASGARRIMVLGS"
     gene            complement(5655..6545)
                     /locus_tag="PK264_15255"
     CDS             complement(5655..6545)
                     /locus_tag="PK264_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77265.1"
                     /translation="MPLDPSHADIISRLERIAAAIERLAPEKSPSPDLDAAEAFVWQS
                     SPEGFLPVPRVNRVPLDLLKGLDRSQELLLDNTMRFAKGLPANNALLWGARGMGKSSL
                     VKAVHAEVRRHLKANRARSDLKLVEIHREDIGALPQCLGHIRASRHRFIVFCDDLSFD
                     RDDTSYKSLKAVLEGGIEGRPENVVFYATSNRRHLMPRDMMENERSTAINPSEAVEEK
                     VSLSDRFGLWLGFHNGTQDQYLDMIRGYAAHHKLPIKDDELVREALEWSITRGGRSGR
                     VAWQYIQDLAGRLGKTLESE"
     gene            complement(7043..8329)
                     /locus_tag="PK264_15260"
     CDS             complement(7043..8329)
                     /locus_tag="PK264_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD77266.1"
                     /translation="MVSLVAVGLLLLFLLLGFPIYLSLGFLALLLFVHEGTPLISLPQ
                     LLVDHLNSETLLAVPLFVIAATFMQRGGVAGALIDCAQAWVGRSRGGLALVCVVACTI
                     FAAICGSSVATAVAIGSVLIPAMTDRKYEPHFALGTTAAAGTLGILIPPSLAMIVYAV
                     IAEESVPRLFLAGVIPGLMQAALFLIWIMVYARWKNYPVEPPIGRQKFVADNVRALPA
                     LLIPVIILGGIYSGLMTVTEAAGVAALVAIIEGVFVYRGVKMKEVAGVLAESIKLTAT
                     IVFIIVGALAFGHWITSAGIAEALVKFVKGMELKAWQFLLFINVLMLFLGMFLEVFAI
                     ILIVMPLILPILKALDINLVHFGIVLTINMEIALISPPVGLNLFVLASVSKQPISTVI
                     RGVMPFLLIMLALLALVTYVPIISTWLPDLVYGKPK"
     gene            complement(8354..8860)
                     /locus_tag="PK264_15265"
     CDS             complement(8354..8860)
                     /locus_tag="PK264_15265"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD77267.1"
                     /translation="MLERIENALMALFATCALCLVCYEVVVRYYFPKFLTDYGLELTI
                     YFTVWAIFIAGAPLVREGRHVRADLLLHMLPAQAQRWLEVLSLLVGLAFIGLLTYFGW
                     RMVHQAFTLGEKSESSLRMPLYLYYASLPVGTTLMIWPFIKRIYDYVFHFDPATMTVT
                     DEHVARDK"
     gene            complement(8943..9998)
                     /gene="dctP"
                     /locus_tag="PK264_15270"
     CDS             complement(8943..9998)
                     /gene="dctP"
                     /locus_tag="PK264_15270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRD77268.1"
                     /translation="MAKRFAPGRGAVESVVTKRTFLGLAAAGAAVGFPNIARAQTPLR
                     LEISLETGPNHIRNIGMTEYADELMKKAGGKLDVKIFHGAAKFKDTDVPKALTQGALD
                     MGLPGTWQLGKYVEDFDAPDLPIFYGAKRHAVYKVWDGPAGAHVVEKIEKKLGVKVIG
                     PWLDLGSGQTFTVSKAIKTHADFTNLKVRIPGGSANIARYQVLGANPIKIAWPDVPQA
                     LQRGTIDGLFTTFESTRSAKLWDSGVRHCYVNDQTFLQYLPMISAKAWASYPKEIQEL
                     IVGTWGGMVNTLRDRADERQNSARADAIKNGITVVDPSKEDIAESRKKLLAKQDALVA
                     ELKIDPELIKKVQAILT"
BASE COUNT         1765 a         3112 c         3337 g         1689 t
ORIGIN      
        1 ctcgaccgac gaggccgacg tcgagcttgc gaccgcgctc aacctcgatc gcgagatcgc
       61 cttccgctcg agcctgctca atgcgcggct cgtgcgctac cgcattccgg ataaccggcc
      121 tatcgcggag gtgctggccg cgatcgccac ccgcgccggc gtgatctcgg cgagccccga
      181 gtacatctat cggccggtgc agggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn ngcctgtccc ggaagcgcat ggcacgtcga tcgccggcat cctcgttgcc
      421 cgcaatcaga tgaagggcat cgcgccggaa gcccgcgtcg tctcggtccg ggcgttctcg
      481 tcggccggtg cgggtcgttc gccggaggcg accacgctct ccgtgatcaa gggcctggaa
      541 tgggccttca tcggcggcac gcgtctttac aacctgagct tcaccggccc gcgcgatccg
      601 ctgctcgagc agctcctcga gcgcgccgaa gcgcagggcg ccgtgttcgt cgcggcggcc
      661 ggtaacgcgg ggcccggcgc gcccgcggcc tatccggcgg cctacaagac ggtggtcgca
      721 gtgactgcgg tcgatgccaa cgatcgcgtc tatgcgcgcg cgagccgtgg gccctacgtc
      781 gcgatcgcgg cgcccggcgt cgacatcctc gcgccgacgc ttgcgagcgg ctacggcatg
      841 tcgtctggca cgtcgatggc cgccgcacac gtgaccgggg ccatcgctct cgtcatggag
      901 aagcggcccg atctgaagcc agcccagatc cgcgaggtgc tcctgcgcgc ggtgcgcccc
      961 ggcgatgcgg ccagtgcccg cgacatcggc gccggcattc ttgacgcagc gaagctgatc
     1021 gaggttgtcg cgaggtaact ccgcgagcga gttgcgggcg atcggcggaa tgtgttctct
     1081 cggggctcga cgttcagtgc gagcaccggg agaagttaca tgcgcctcga gggcaagatt
     1141 ggcatcgtga cggcggcggc ctccggcatg ggacgcgcgg gcgcgctgcg ctttgccagg
     1201 gaaggcgcct cagtcggcgt tgtcgatatc gatgcgaagg gcgtcgagag cgtggtcgcc
     1261 gagattcgcc gtgctggcgg cagcgcgcac gcgatcgtcg ccgacctcac gcgggacgag
     1321 gacagccgac gcatcgtcaa ggacaccgcc aaggccttcg gcgggctcga tttcgtctgg
     1381 aaccacgtcg gccacccggg cccggcctcc ttcgaaggtc tcgacctcga ggaatgggac
     1441 aacgccgtcg cactgaacct tcgcaccgtc ctcgtcacca gcgaggccgc cattcccgag
     1501 atgcgggcgc gcggcgccgg cgccatgctc tacacggcgt cgacgtcggg catctccggc
     1561 tcgcagttca gtccggtcta ctccgccgtc aagcacggcg tggtcggcct cgtgcgcggg
     1621 ctcgccaagc gttatgccaa ggagcgcatc cgcatcaacg cgatctgccc gggcccgatc
     1681 gacaccccca tgctgcgcac gttcgtcgct cgtcccgacc aacagtcgac cctcggcatg
     1741 gacaaggagg agctcgtcag gaagcgcgcc taccagacgc cgatgggccg gccgggacag
     1801 cccgaggagg tcgccaacgc cgcgctcttc ctcctctcgg acgaggcgtc gttcgtcacc
     1861 ggcgcctgcc tgaccgtcga tggcggcgcg acctgctgag gcagcaggca ggggtagccg
     1921 gttcggctga cgcggcccgc cgacgcgatg gctcgcgcaa caacactatg tttgctgctg
     1981 cacggatttt cgccattggc ggagcaatat tgttgcggag ctggctggat ttgcatccag
     2041 ccacggcact cttaggcccg catctcatcg accggcaacc ccaacgccac gcgcccggca
     2101 agcttgcgtt gttcggcggg gcgcagggga tggaaacgcg cgccctggat atctcggaac
     2161 agccgctcca gcccttgctt tcggaagtag ctcgcgccgc cagcgacctc gagggccaac
     2221 tcgacagtcc tgagcgcgct gcgggcaatc agactgcgat aggtgaagat ttcactggtc
     2281 gtcgcctcgc ccggttgcgc cactgccgcg aatgccacca tgctttcgtg ggcgacacgc
     2341 gtcgccgtcc actcggtctg cagttcgccg accagatcga cggagggcgt tccctttcgc
     2401 ttcgccgctg ctatggcgat ctcgcaggct gcctcggcga taccggtgta gacggcatag
     2461 accagcggga aggccaccat tgagatggtg tgcatgatag ggtgccacgc gccagacggc
     2521 cggcgaaccg atacagcagc atctggcacg aagacatttt cgaacaccac gtcatgcgag
     2581 gccgtgccgc gcatgcccat cgtgtcccag gtctcgacaa tgctgatgcc gggtgaagtg
     2641 aggggcacgg cgatgtgcat caccatcggt ccctccggcg tctgctccac cgccatcgtc
     2701 atgaacaggt tggctgacgg cgcaccgctg gcaaagacct tacgtccatc gattttatag
     2761 ccgccctcga cctttgtcgc gattcccgag ccgggcagcc aatccgagcc accggacgtc
     2821 aggatttgca gtcgctcggc tgcgatccgc ctcaaaaggg cgtcagtcgg tgctttctgg
     2881 tgtcgccacc gccaggccgc aaaggccacc ggatgtgtgt gcatcgacat ggtcagtgct
     2941 gtggcgctcg aggtacgcgc cagcgtgcgc aggaattcgg ccacctccgc aggcagcaat
     3001 ccggctccac ccaactcgct cggtacagca agggcaaaga acccgtgctc acgcagcagc
     3061 gccatgttgt ccgcgacgta gtccttggtc tggtcgcaac gcctttcggt ggcctccaat
     3121 cggggtgcca gctcagccaa ggtcgcgcgc cagtcgaagg acgtttcggg gggagtgctt
     3181 gatgcagttg cagcagacat gaaaatctcc tttgatgcgc attccttgta cggaaacttg
     3241 tgtcaatcga ggtgcttcgg caatgaccga aaagccgggt ttacctgccg agacgccgga
     3301 tgtcttgtcc gacatgctgc gcgccgtacg cctgaccggt gccgtgttct tcagcggtcg
     3361 ctacaccgcg ccatatggaa ttcggtcgac caaacggttc gacccgagca tgccggcagc
     3421 acatttgcgc catatcagcg tcttccacct tctcacggcg gggacctgca tgttcgagtc
     3481 ggcgagtggc agccaacgtc ggctcacggc cggcgacctc cttctgatcc cgtttgccgg
     3541 tgagcaccaa ttctgggagg gcgacgacgt ccgcatcgtg ccggtggaaa acaccgtccg
     3601 ccaaggccac catcccggtc tgtggacatc cgaccatggt ggcagcggtc cggagacacg
     3661 cctcgtttgc gggctcctcg agtcgtcgga attccttgcc atgccgctgt ttcgcacact
     3721 tcccgagttc gtcatcgcgc gaccaggtga cttcaaggcc ggggaactgg tggtcgccac
     3781 ggtgcgggac atcatggcac ttgtcgacgc cgcttcccct ggtgccgaca tcatgctcgg
     3841 ccgcatgatg gagctccttt tcatcgagat gctgcgccgc catgcctcga gcctgcctca
     3901 agacagcagg ggactattgg ccgcgatgaa cgaccccgtc gtcggccaag ccctgaagct
     3961 ggtccacgcg gaacctgcac gccactggac ggtcgacagc ctcgcgcgca agaccggtgc
     4021 atcgcgcacg gtcctggccg agcgcttcaa tgctttcctc gggcgcccgc cgatcgacta
     4081 cgtcatcggc tggcgcatcc agcttgcggc ggagcgcctg cgcaatggac gcgatccgat
     4141 cgcgaccatc gcggccgacg tcggctatga atcggaggca gccttcggtc gcgccttcaa
     4201 gagggtgact ggcatcagcc ccgggcgctg gcgcaatggc gccggtgaca gtcccgacct
     4261 gatgcctctt cagttcaaga cgccgttagg tccggttgcg gacgatgcag ccgtagaatg
     4321 agttgcgctt gtgtcgcgag ccatgcgcaa cgccgtcagc ctcaagcgct cacgggccgg
     4381 cggcaggcgg agatgagggc gagcacggcg aggaagccgg cgaaggcggc ggcggcagcg
     4441 aaccacatgg cagccatcgg tgagacgcgc gcgatgagcc aggcgaatat cacgggtgcg
     4501 gttgcgttga cgatgttctg cggcaccatg aggaggccca tgtagctgcc gtagtcgcgt
     4561 gcgccgaaga gcgtcagcgg cagcgtgttg cgggcgatgg cgaagaggcc gtgcccgaag
     4621 ccgatcgcga acgagaagac gatcgccgtc gtgatgctga cgccgaacgg cagaaaggcg
     4681 agaaaactcg ccgtcgtcag caccgcagac gcgagcgcgg tcgtgtagat cgaatgcttg
     4741 tgggcgaaga cgagctcggc gaagcgggcc gcggcctgcg ccggtccgac gatcatccag
     4801 atgaggatcg cgtcaccctt gccgatgccc tgggcctgca gtaccaggat ctggcnnnnn
     4861 nnnnnggaga tagagccccc acgtaatgag cccttccgtg cacgaccagg tggcgagcat
     4921 gaagagcgcg cgccggcgat gctcgggcgg caggggggcc tcggcaggcg cggccgctcc
     4981 ggagcgcgcc ggggaacggc cgtcgcccga ggcctcggcg cgatgcatga tgagggcatg
     5041 cacgggaagg cagatgccgg catgcaggat ggcgaacacg atgtaagttc cgcgccagcc
     5101 gaaaaggtcg gcgagcttcg agatgagcgg caggaagatc gtcgaggtga ggccgctcag
     5161 cagcatgagg ccggtgatgg cgcgccgcgc gttcggccnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 ggagcccaga accatgatgc ggcgcgcccc gctcgcatcg acgcgcttgc cgatgcgggg
     5401 ggcaaagagg gcgctcacca gcagcatcac tgtgatgccg gcgaacacga cctcgcgcgg
     5461 cgcctggata tcgtggccga tcggagtgcc gaggacggtg agcggcgaga agatgctgcc
     5521 ccagccgatg atctgcgtga cgccgagagc ggcaacgatt accggcgggc ggcggacgat
     5581 tgcgaggctc tgggcgctga tcacggtcgt ccccctccgc tgcccaggcc acgattgcgg
     5641 tcagatcgcg gtcatcactc gctttcgagc gtcttgccga gccggccggc gagatcctgg
     5701 atgtactgcc aggcaacgcg ccccgaccgg ccgccgcgcg tgatgctcca ctcgagcgcc
     5761 tcgcggacaa gctcgtcgtc cttgatcggc agcttatggt gtgccgcata gccacggatc
     5821 atgtcgagat attggtcctg ggttccgttg tggaagccaa gccagaggcc gaagcgatcc
     5881 gacagcgata ccttttcctc gaccgcctcg gagggattga tggcggtcga gcgctcattc
     5941 tccatcatgt cgcggggcat caggtgacgg cgattggagg tggcgtagaa aacgacgttt
     6001 tccggccggc cctcgatgcc gccctcgagc acggccttca gcgacttgta ggaggtgtcg
     6061 tcacggtcaa aggagaggtc gtcgcagaag acgatgaagc ggtgtcggct cgcgcggatg
     6121 tggccgaggc attggggcag cgcgccgatg tcttcgcggt ggatctcgac gagcttcaag
     6181 tcggacctgg cccggttggc cttcaggtgc cggcgcacct cggcgtgaac agctttcacg
     6241 agactggact tgcccatgcc gcgggcgccc cagagcagcg cgttgttggc gggcagcccc
     6301 ttggcgaagc gcatcgtgtt gtcgagcagc agttcctgcg agcggtcgag gcccttcagg
     6361 aggtccagtg gaacacggtt cacgcgggga acgggcagga atccttcggg gctcgactgc
     6421 cagacgaacg cctcggcggc gtcaagatcg ggggaaggtg atttctcggg ggcaaggcgc
     6481 tcgatggccg ccgcgatgcg ctcgaggcgc gagatgatgt cggcgtggct cggatcgagg
     6541 ggcattcgtc tctccggatg ggcggcgcgg gttcgcggcc ggttctatag acgaggccgc
     6601 tcgggcctgt caccatcgga gcatgcaggg gagggccgct ttgagcgagc cctcagttag
     6661 ctcaggaaca tcgcgacgta gctgccgccg gcgagcgtgg cggccatgac gacgaagcag
     6721 ctggtcctga cgatctctgc gaacatgaca ctctccctcg gcattctttc tggttttcga
     6781 tcaaggtagc gaaacttcgt gaaggtccgt tgaacagctt gttcaggatt gtgtgctcag
     6841 cgtcccgaca cgagacattt gcagccacca gagcggtttt gctgtgcccg tcggcacaag
     6901 cgccaaaaga agagattttc cagggttctg gagacgcggc cgcaagcccg ccccgaagac
     6961 gaggggcagc ctgcgccgag gccagggccg cggcagcgcc aaagggtgct tcgggatcga
     7021 tgagcggcgg gtctggaagc cgctacttgg gcttcccgta gacgagatcc gggagccagg
     7081 tcgagatgat cggcacatag gtgacgagcg ccagcagggc gagcatgatg agcaagaagg
     7141 gcatcacgcc gcggatgacc gtgctgatcg gctgcttgga gaccgaggcg aggacgaaca
     7201 ggttgagccc gaccggcggc gagatgagcg cgatttccat gttgatcgtc agcacgatgc
     7261 cgaagtgcac gaggtttatg tcgagcgcct tcaggatcgg caggatcagt ggcatgacga
     7321 tcaggatgat cgcgaacacc tcgaggaaca tgccgagaaa cagcatcagt acgttgatga
     7381 acagcaggaa ctgccaggcc ttcagctcca tgcccttcac gaacttgacg agcgcctcgg
     7441 ctatgccggc ggacgtgatc cagtgaccga aggcgagcgc gccgacgatg atgaagacga
     7501 tcgtcgccgt gagcttgatc gactcggcaa ggacaccggc gacctccttc atcttcacgc
     7561 cgcgatagac gaacacgccc tcgatgatgg cgacgagcgc ggccacgccc gccgcctccg
     7621 tcaccgtcat gaggccggaa tagatgccgc cgaggatgat cactggaatg agcagcgccg
     7681 ggagcgcgcg gacgttgtcc gcgacaaatt tctgccggcc gatcgggggc tccacgggat
     7741 agttcttcca gcgggcgtag accatgatcc agatgaggaa cagtgcagcc tgcatgaggc
     7801 cggggatcac gccggcaagg aacagccgcg gcaccgattc ttccgcgatc acggcataga
     7861 cgatcatggc gaggctcggc gggatcagga tgccgagtgt cccggcagcg gccgtcgtgc
     7921 cgagcgcgaa gtgcggctcg tacttgcggt cggtcatcgc gggaatgagc accgagccga
     7981 tcgccacggc cgttgccacg gacgagccgc agatggcggc aaagatggtg caggcgacga
     8041 cgcagacgag cgcgaggccg ccgcgcgagc ggccgaccca ggcctgtgcg cagtcgatga
     8101 gggcgcccgc gacgccaccg cgctgcatga aggtcgccgc aatcacgaac agcggcaccg
     8161 cgagcagcgt ctcggagttg aggtgatcga cgagcagctg cggcagcgag atgagcggcg
     8221 tcccctcgtg cacgaacagg agcagcgcca ggaagccgag cgacaggtag atcggaaagc
     8281 ccagcagcag aaagagcagc agcagcccga cggcgactaa ggaaaccatg gatgtagctt
     8341 ctcgctgtct ggattacttg tcgcgggcga cgtgctcgtc cgtgacggtc atcgtcgccg
     8401 gatcgaagtg gaagacgtag tcgtagatgc gcttgatgaa cggccagatc atcagtgtag
     8461 tgccgaccgg cagcgaggcg tagtagaggt aaagcggcat cctcagcgag ctctcgctct
     8521 tttcgccgag cgtgaaggcc tgatgaacca tgcgccagcc gaagtaggtg aggaggccga
     8581 tgaaggcgag gccaaccagg agcgaaagca cctcgagcca gcgttgcgcc tgcgcgggca
     8641 gcatgtggag aagaagatct gcgcgtacgt gccggccttc ccgaacgagc ggcgcgccgg
     8701 cgatgaagat tgcccatacg gtgaagtaga ttgtgagctc gagcccataa tctgtcagaa
     8761 attttgggaa ataatacctg accaccacct catagcagac cagacacaag gcacacgtag
     8821 cgaacagcgc catcagcgcg ttctcgatcc gctcgagcat ggcgggctcc gcaagattgg
     8881 caggtcctca tgcgccaaca gccggctcgg tgagccggct gtgcaaggca catatcgcaa
     8941 ggtcaggtga ggatggcctg aaccttcttg atgagttcag gatcgatctt cagctcggcg
     9001 acgagcgcgt cctgcttcgc caggagcttc ttgcgcgact cggcaatgtc ctccttgctc
     9061 gggtcgacca cggtgatgcc gttcttgatg gcatcggcgc gcgctgaatt ctgccgctcg
     9121 tcggcgcggt cgcgaagggt attcaccatg ccaccccagg tgccgacgat cagctcctgg
     9181 atctccttcg ggtagcttgc ccaggccttg gcgctgatca tcggtagata ctggaggaag
     9241 gtctgatcgt tcacgtagca atgcctgacg cccgaatccc agagctttgc gctgcgggtc
     9301 gactcgaagg tcgtgaacaa gccatcgatg gtgccgcgct gcagcgcttg cggcacgtcc
     9361 ggccaggcga tcttgatcgg gttggcgccg agcacctgat agcgggcgat gttggcagag
     9421 ccgccgggga tgcgcacctt gaggttggtg aagtcagcgt gcgtcttgat cgccttcgag
     9481 acggtgaagg tctggcctga accgaggtcg agccaggggc cgatcacctt gacgccgagc
     9541 ttcttttcaa tcttctcgac gacgtgggcg ccggctggac cgtcccacac cttgtagacg
     9601 gcgtgccgct tggcgccgta gaagatcggc aggtccggcg cgtcgaagtc ctcgacgtac
     9661 ttgccgagct gccaggtgcc cgggaggccc atgtcgagcg cgccctgcgt gagtgccttc
     9721 ggcacgtccg tgtccttgaa cttggcggcg ccgtggaaga tctttacatc gagcttgccg
     9781 cccgccttct tcatcagctc atcggcatac tccgtcatgc caatattgcg gatgtgattg
     9841 gggccggtct cgagcgatat ctcgagacgg agaggcgtct gcgcgcgcgc aatattcggg
     9901 aagccgaccg ccgcgccggc cgcagcaagc ccgaggaaag tccgcttcgt gaccacgctt
     9961 tcaacagccc cgcgacccgg agcaaacctc ttcgccatga catcctccct gtgcgcagcg
    10021 tcttcaagtg ccgcgtcttg gcggcgtttg cgagcgctga cggcacgtta gacatgatgc
    10081 tcgtggtgtc aatgccgcgc tcagggcgcg ggcgcgactt gccagcgcgg cgagatcggc
    10141 gccgtggagt tcgagatagt cgagcttgcg gtcgcgcggc atcgtcggtc tccccgtcgg
    10201 atgttcttgt tatgttctag
//
LOCUS       DAORNH010000156        10160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4901_length_10160_cov_2.625136, whole genome shotgun sequence.
ACCESSION   DAORNH010000156 DAORNH010000000
VERSION     DAORNH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4901_length_10160_cov_2.625136"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PK264_15275"
     CDS             complement(<1..813)
                     /locus_tag="PK264_15275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD77269.1"
                     /translation="MRRGGTRAASLQEGAGFAALLAIVALLTSLPLGRLATAALAPEG
                     RWSPANALAEISTKAAVQATWNTIEVGVLSGLGALVIGTLFAVLVGVTDVRGKRPLAF
                     LFVLSLLVAPQVAALAFKSLLGPGSPLLEALGLALPAGTANPMVGRGGIIAVLALHHA
                     PLVAVTLMPGLRAIPRTMIEAASLDGAGPILIARRILLPLLRHHLAAAGLLAFVAGAG
                     NFGIPALLGLPANYLTLPTLIYRRLSSYGPSVLADVAALAMLDPEFGDLTTDA"
     gene            complement(834..1787)
                     /locus_tag="PK264_15280"
     CDS             complement(834..1787)
                     /locus_tag="PK264_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD77270.1"
                     /translation="MLSALSVPAPAAAQEGRLVLYTSQPNTDAQQTIAAFKAKFPKVD
                     IVFVRDGTPRIMAKLRAEIAAGGSQADVLLIADSVTMEGLKAEGLLLAHPKADIAAYP
                     AGLHDKDRYWFATKLITTGIIYNRKAPKRVASWNDLKSAESKGQVVMPSPLASGAALI
                     HAASLVAALPAGWGYYEALKGNGAVAASGNGDVLKQVATGEKLYGVIVDFMALREKAK
                     GAPIEFVFPTEGVSAISEPVAVLKSTKNPAAALAFIDFILSTEGQTLARSQGYYAARP
                     GIAPPDGYPPISGVRILPLDPAAALASARENAKRFADIFGQ"
     assembly_gap    1789..1815
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2008..2412
                     /gene="yidD"
                     /locus_tag="PK264_15285"
     CDS             2008..2412
                     /gene="yidD"
                     /locus_tag="PK264_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRD77271.1"
                     /translation="MSSTLAVAAIHIYRCGISPFKGFRCAHDVLHRSGSCSDYGLEVF
                     RANGLRAGISLMRQRFADCRAAFGALQVAAASTSNPEGEGGQTKKKNSDACWCDIASG
                     AADIASCGGRSLRVGGDFGEACSSVDVCACSW"
     gene            complement(2422..3399)
                     /locus_tag="PK264_15290"
     CDS             complement(2422..3399)
                     /locus_tag="PK264_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRD77272.1"
                     /translation="MTSLQDRGRIGHQRFGVSPAGAMDPLALAVANALVGNPLDTAAI
                     EMAVFGAALKAEGGSVRVALAGAEMALEVGGEPVPPLASVTLREGERLTIRSARSGVF
                     AYLAVAGGFDVPPDLGSLSHHARSGLGPKRIESGTRLAVAGGEPSGPERQLVGGLLPA
                     STAPLAVILGPQDDYFSKAGIATFLSATYTISTEADRMGYRLTGPKIEHEKGFNIVSD
                     AIVTGSIQVPGSGEPIVLMTDRQTTGGYPKIAVVTRADLPRLAQMRPGSTVRFTAVSR
                     TEAVERLRAEARRLAEIVGRISAAGADLLASERLLGLNLAGDAAGVDHH"
     gene            complement(3437..>4526)
                     /locus_tag="PK264_15295"
     CDS             complement(3437..>4526)
                     /locus_tag="PK264_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HRD77273.1"
                     /translation="MSLINCPEIEVIIEATGDPATGIKLAMAAINAGKHVVMVNVEAD
                     SLAGPLIAKRAREKGVVYSLAWGDQPALVCEHVDWARSCGFEVIAAGKGTRYHPTYHQ
                     STPESVWAILDTYLKIHDKSHINPKMFNSFVDGTKSGIEMTAICNATGLVPQTDGLSF
                     PPASRFELSEVCKPKADGGVLEKKGVTEVTSSLYRDGKDVPHNLVMGTYVVISSDSDY
                     TQKCFAEYSMLPDKSGKYAALYRPTHMIGLELGLSVASAALRKEPTGSPFCFNSDVVA
                     TAKRALKKGEMLDGEGGFCVWGKQTPASMSLNEGYLPLGLAHNVKLKGDIAEGQRIKW
                     SDVAYDATDSAVKFRKEMEAVFGRPNVG"
     assembly_gap    4527..4649
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4650..4787)
                     /locus_tag="PK264_15300"
     CDS             complement(<4650..4787)
                     /locus_tag="PK264_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgA"
                     /protein_id="HRD77274.1"
                     /translation="MNLSFMLKDRAAANRPVRIGQVGAGKFGTMHLSQCRLTTGMHLT
                     GL"
     gene            5349..>5614
                     /locus_tag="PK264_15305"
     CDS             5349..>5614
                     /locus_tag="PK264_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilotriacetate monooxygenase"
                     /protein_id="HRD77275.1"
                     /translation="MSQPKMMSLAILVDQATGNHPASWLQPEVNAKASIDIGHYRHLA
                     RLAEAGKFDLFFIADTPAARTENFQAWSRFPMYMNALEPVTLLTA"
     assembly_gap    5615..5635
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5636..6688
                     /locus_tag="PK264_15310"
     CDS             <5636..6688
                     /locus_tag="PK264_15310"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855639.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HRD77276.1"
                     /translation="GLGGTVSTSFSEPYNVARQFASLDHISGGRAAWNVVTSANDYAA
                     RNFGHAKLAPHAERYERAGEFVDVVTKLWDSWDDDAFVRDRATSRYFDPARMHTVHHE
                     GRFFKVVGALNIERPPQGHPVISQAGASDTGRELAARTAEVVFASAAAPEPAKAAYDD
                     LKGRMAKYGRAPETLKVLAGLPVVIGQSRAEAEERFERLQGLLHPDVGLFRLAMDLEV
                     DLTGLPLDEPVPESRLPKGANFHKAFFDGIMASIRDEKPTLRQLYMRYERGRKTIKGT
                     PKDIADIMEHWLHSGACDGFMLQFHTLPQSLDEFVHLVVPELQRRGIYRRDYAGPTLR
                     DHLGLARPANPNTVRH"
     gene            6691..>6884
                     /locus_tag="PK264_15315"
     CDS             6691..>6884
                     /locus_tag="PK264_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77277.1"
                     /translation="MAVLFAQVLVATTGLAHALASWQYFARISSRGCWIWPLAWAIGT
                     LASLVLMQSAPLAGFTAFLAL"
     assembly_gap    6885..6914
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6915..7731
                     /locus_tag="PK264_15320"
     CDS             <6915..7731
                     /locus_tag="PK264_15320"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRD77278.1"
                     /translation="RASTTLEWVAENRLQATGRIIGNEVAITNVRNFDWIDKRTFEEK
                     WEARQYDIETLQAIDVFVSSWGNPRIAHIMVSFDFAGGPPLCVSVETRREAGERWSAL
                     AGFMKSYELIIIAGDERDLVRSRVNVRGEDVRLYRIYTTPEMRRKILAGTIAQMNRLS
                     ARPRFYNTIFHNCTIEIARIVWAAGHRFPFDWRLLVSGYVAEYLYDVGLLDRTLPFAG
                     LKTSADITQRSRTADRAPDYSQRIRAGLRDPTRDIAVTASPVSPARPQVGARP"
     gene            7728..8777
                     /locus_tag="PK264_15325"
     CDS             7728..8777
                     /locus_tag="PK264_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD77279.1"
                     /translation="MTSPRSLPPSHHLPQRPEWLAKVVEEIIEPDLPIIDPHHHLWER
                     EGNRYLLDDILEDTASGHRIASTVFMECRAMYRQGGDPELACLGETEFVNGIAAMSAS
                     GSYGPTRVAEAIIGRVNLMLGAGARRILEAQMAVAGGRFRGVRYITVSHPDPAARGAS
                     TNQEPGILRDPRFRASFAELAPLGLTFDAWMYHTQLDELADLARTYPATTIIVDHVGG
                     PLGLGPYQGKRAEVMAEWTKSLKGLASCPNVYMKLGGLGMRFPGFDFHEREMPPTSKQ
                     LAEAWSPYLETCISVFGASRCMFESNFPVDKPTCSYQVLWNTFKRVAAKATAEDKRAL
                     FSGTAAKVYRLQPAV"
     gene            complement(8784..9497)
                     /gene="aat"
                     /locus_tag="PK264_15330"
     CDS             complement(8784..9497)
                     /gene="aat"
                     /locus_tag="PK264_15330"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRD77280.1"
                     /translation="MASREDIAIEITPQVLLKAYACGIFPMAESADDPALYWIEPQHR
                     GILPLDHVHVPKRLARTVKAGTFEVRIDYDFRGVMDGCAASRPGRRTTWINDRIRHLY
                     GALYDLGHCHTVETWSGGQLVGGLYGVALGGAFFGESMFSTERDASKVALVHLAARLI
                     HGGFTLLDTQFVTDHLRQFGTVEVDRDAFHGLLEKAVHVKADFNRLRRDAPPARWLEI
                     IAAHHPAAIATPPDDDGSG"
     gene            complement(9661..>10160)
                     /locus_tag="PK264_15335"
     CDS             complement(9661..>10160)
                     /locus_tag="PK264_15335"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRD77281.1"
                     /translation="GPASVAFNSSYASGSVVIDTSGRALYLVTSSTTAYRYPISVGRE
                     GFTWTGTERISRVASWPDWHPPAEMRERDPRLPQKMTGGIRNPLGAKALYLGNSLYRI
                     HGTNDAASIGRAASSGCFRMMNGHVVDLADRIGVGTTVHVVSRLPANVAMDAGSAPRR
                     TASTR"
BASE COUNT         1691 a         3342 c         3291 g         1635 t
ORIGIN      
        1 cgcatcggtc gtgagatcac caaactccgg atcgagcatg gcgagggccg cgacgtcggc
       61 gagcacgctc ggcccatagc tcgagagccg ccgatagatg agcgtcggca aggtcaggta
      121 gttggccggc aggccgagca gcgccggaat gccgaagttc ccggccccgg cgacgaaggc
      181 gagcagcccg gcggccgcca gatgatggcg gagaagcggc agcaggatgc gccgcgcgat
      241 caggatgggc cccgccccgt cgagcgaggc ggcctcgatc atcgtccgcg gaatggcacg
      301 cagccccggc atcagcgtca cggcgacgag cggcgcatgg tggagcgcca gcacggcaat
      361 gatcccgccc cgcccgacca tgggatttgc cgtaccggcc ggcagggcca gtccgagcgc
      421 ctcgagcagc ggcgagccgg gaccgagcag ggacttgaag gcaagtgccg ccacctgcgg
      481 ggcgaccagc agagagagga cgaaaaggaa ggcgagcggc cgcttgccgc ggacgtccgt
      541 cacgccgacg agcaccgcaa agagcgtgcc gatgacgagc gcgccgaggc ccgagaggac
      601 gccgacctcg atcgtattcc aggtcgcctg aacggcggcc ttggtcgaga tctcggcgag
      661 cgcattcgcc gggctccagc ggccttcggg cgcaagggct gccgtggcga ggcggccgag
      721 cgggagcgat gtcaacagcg cgacgatggc gagcagtgct gcaaatcctg cgccctcctg
      781 tagcgacgca gctcttgtcc cgcccctcct catcgacctg acgcgatcct tcactattgt
      841 ccgaagatgt cagcgaagcg cttggcgttc tcacgcgccg aggcgagtgc tgcggccgga
      901 tcgagcggca ggatgcgcac tcccgagatc ggtgggtagc cgtcgggcgg cgcaattccg
      961 ggccgcgctg catagtagcc ctggctgcgg gccagtgtct gaccttcggt cgacaggatg
     1021 aagtcgatga aggcgagcgc tgccgcaggg ttcttggtgg atttcagcac cgcgaccggc
     1081 tcgctgatcg ccgacacgcc ttccgtgggg aagacgaact cgatcggtgc gcccttcgcc
     1141 ttctctcgga gagccatgaa gtcgacaatg acgccgtaga gcttttcgcc ggtcgcgacc
     1201 tgcttcagca cgtcgccgtt gcccgaggcc gcgacggcgc cgttgccctt caatgcttcg
     1261 tagtatcccc agccggcggg cagggctgcg acgagcgagg cggcatggat cagcgccgca
     1321 ccggacgcga gcggacttgg catcaccacc tggcccttgc tttcggcgct cttgaggtcg
     1381 ttccacgagg ccacccgctt cggtgcctta cggttgtaga tgattccggt cgtgatgagc
     1441 ttggtcgcga accagtaccg gtccttgtcg tggagcccgg cggggtaggc tgcgatatcg
     1501 gctttcgggt gggcgagcag cagcccctcg gccttgagcc cctccatggt gacgctgtcg
     1561 gcgatcagca gcacgtcggc ctgagacccg ccggcggcga tctcggcgcg cagctttgcc
     1621 atgatgcgcg gcgtaccgtc ccgcacgaag acgatatcga ccttcgggaa cttcgccttg
     1681 aaggccgcga tggtctgctg ggcgtcggta ttcggttggc tcgtatagag cacgaggcgg
     1741 ccttcctggg ccgcagcggg ggccgggacg gaaagggcgg acagcagcnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnncatca ggcgtagcgt catggttgaa ccatcttggc aacagtgatc
     1861 ggtcggcagc acggcggatc gcgcttcact acatttagta gatgcgaagc gcgacggcgg
     1921 tgcgacaaac tcggactgcg ggccgcaagg attcggttcg atgtattggg gcgggcccgg
     1981 gctgggactt ggggcaagga gccgggaatg agcagcacat tggcagttgc cgccattcat
     2041 atctatcggt gcggaatttc gcccttcaag ggcttccgct gtgcccacga cgtgctgcat
     2101 cgctccggaa gctgctcgga ctatggcctc gaggtttttc gcgccaacgg cctcagggcc
     2161 ggcatttcct tgatgcggca gcgctttgca gactgccgcg cggcatttgg cgcgctgcag
     2221 gtggcggcag cctccacgtc caaccccgag ggcgagggcg gtcagaccaa gaagaagaac
     2281 agcgacgcct gctggtgcga catcgccagc ggcgcagccg acattgccag ttgcggcggg
     2341 cgcagcctgc gcgtcggcgg cgatttcggt gaggcctgca gctcggtcga cgtgtgcgcg
     2401 tgctcgtggt gaccgtggcc gtcagtgatg atcgacgccc gcggcatcgc ccgcgaggtt
     2461 gaggccgagc aggcgctcgc tcgccagaag atcggcgccg gccgccgaga tgcgcccgac
     2521 gatctccgca agccgccgtg cctcggcccg tagccgctcg accgcctccg tccgcgatac
     2581 ggccgtgaac cgcaccgttg accccggtcg catctgcgcg agtcgcggca ggtcggcacg
     2641 cgtcacgacc gcgatcttgg gatagccgcc ggtcgtctgc cgatccgtca tcagcacgat
     2701 cggctcaccc gagcccggca cctggataga tcccgtgacg atcgcatccg agacgatatt
     2761 gaagcccttc tcgtgctcga tcttcgggcc ggtcagccgg tatcccatgc gatccgcctc
     2821 ggtcgagatc gtataggtcg ccgacaagaa tgttgcgatg ccggccttgc tgaaatagtc
     2881 gtcctgcggc ccgaggatca cggcgagcgg cgcagtgctg gctggcagga gcccgccgac
     2941 gagctggcgc tccggacccg aaggctcgcc gccggcaaca gcgaggcgcg tgccagactc
     3001 gatccgcttc ggcccaagac ccgagcgggc gtgatgcgaa aggctgccga gatcgggggg
     3061 cacatcgaag ccgccggcca cggcgaggta ggcgaacacg ccgctccgcg ccgacctaat
     3121 ggtgagccgc tcgccctcgc gcagcgtcac gctggcgagc ggcggcaccg gttcgccacc
     3181 cacctcgagc gccatctccg cacccgcgag cgccacacgg accgagccac cttcagcttt
     3241 cagcgctgcg ccgaacacgg ccatttcgat tgcggccgta tcgagcggat tgccgacaag
     3301 cgcgttggcg acggcgagcg cgagcggatc catggcgccc gccggcgaca cgccgaagcg
     3361 ctgatgcccg atccggccgc gatcctggag cgaggtcatc gctccagcgc tttcgaagac
     3421 gagagttgcc accggatcag ccgacgttcg ggcggccgaa cacggcctcc atctccttgc
     3481 ggaacttcac agcgctgtcg gtcgcgtcgt aggcgacgtc cgaccacttg atgcgctggc
     3541 cttctgcgat atcgcccttg agcttcacgt tgtgggcgag gccaagcggg aggtagcctt
     3601 cattcagcga catgctcgcg ggcgtctgct tgccccacac gcagaagccg ccctctccgt
     3661 cgagcatctc gcccttcttg agcgcgcgct tcgccgtcgc gacgacgtcc gagttgaaac
     3721 agaagggcga gcccgtcggc tccttcctga gcgctgcaga ggcgaccgag aggccgagtt
     3781 cgaggccgat catgtgggtc gggcgataga gcgccgcgta cttgccgctc ttgtccggca
     3841 gcatcgagta ctcggcgaag cacttctggg tgtagtcact gtccgacgaa atcacgacgt
     3901 aagtgcccat gacgaggttg tggggcacgt ccttcccatc gcggtagagc gaggacgtca
     3961 cctcggtgac ccctttcttc tcgagcaccc cgccgtccgc tttcggcttg cagacctcgg
     4021 aaagctcgaa gcgcgaggcc ggcgggaagg agaggccgtc cgtctgcggc acgaggccgg
     4081 tcgcgttgca gatcgccgtc atctcgatcc ccgacttggt gccgtcgacg aacgagttga
     4141 acatcttcgg gttgatgtgg ctcttgtcgt ggatcttcag atacgtgtcg aggatcgccc
     4201 acacgctttc gggtgtcgac tggtggtagg tcgggtggta gcgcgtgccc ttgccggccg
     4261 cgatcacctc gaacccgcac gagcgcgccc aatcgacatg ctcgcagacg agcgccggct
     4321 ggtcacccca ggcgagcgag tagacgacgc ccttctcgcg ggcgcgcttg gcgatcagcg
     4381 gaccggcgag gctgtccgcc tcgacgttca ccatcacgac gtgcttgccc gcattgatcg
     4441 cggccatggc gagcttgatg ccggtcgccg ggtcgcccgt cgcctcgatg atgacctcga
     4501 tctcggggca gttgatgagc gacatgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng agcccggtca gatgcatgcc cgtggtgagc
     4681 cggcactgcg aaagatgcat ggtgccgaac ttgccggcgc cgacctggcc gatcctgacc
     4741 gggcggttgg ccgcggcgcg atccttgagc atgaaagaga ggttcatcgc aggctcccga
     4801 gggtgaattt gcgcgcgacc atatacgagc gacctctcgg gagccaatga atgtgatgac
     4861 ggtcgcccat ccgaaaacga cggggcgagt gcactggcac ggacccgaaa gcaatgtcgt
     4921 tgcacgggtc gcggcgcgaa cctgccgtag tgctcgcccc aagctcgtca tcctcgcgaa
     4981 gacgaggacc cagtgcacct tgtatgggac cgcaatggcc actccgaggt gcactgtgtc
     5041 ccgccctccg gcgggaggac ggtgagtggc tgagaccggc ggcgaccttg ccgcaacgag
     5101 ccgcatgggg ccgccggagt ttagcccatg cctgacacgg gtcggccctg acggggaggg
     5161 gtagcgtgcc aatattgggt cgggtccggg ccgccccgcg cgcggatggc tcgcaaacct
     5221 agggcgaggc ccggtcgtgg agcgagcgtg gcgttgccaa gcccttgccg agggcatgag
     5281 cgaggcgcat gctgccgctc gatccgccgg ggaccgctcc ggccgcgcaa ccgctttaga
     5341 ggccagcctt gtcgcagccg aaaatgatgt cgctcgccat cctcgtcgat caggcgacgg
     5401 gcaatcatcc cgcgtcctgg ctgcagccgg aggtgaacgc caaggcctca attgatatcg
     5461 gccactaccg ccacctcgcg cggctcgcgg aggccggcaa gttcgacctc ttcttcattg
     5521 ccgatacccc agccgcgcgc acggagaatt tccaggcctg gagccggttc ccgatgtaca
     5581 tgaatgcgct cgagcccgtg acgctgctca ccgcnnnnnn nnnnnnnnnn nnnnnggcct
     5641 cggcggcacc gtctcgacga gcttttccga gccctacaac gtcgcccgcc aattcgcctc
     5701 gctcgaccac atctcaggag gccgcgccgc ctggaacgtc gtcacgtccg cgaacgacta
     5761 tgccgcccgc aacttcggcc atgccaaact cgcgccccac gctgaacgct acgaacgcgc
     5821 aggcgagttc gtcgacgtcg tcacgaagct ctgggacagc tgggacgacg acgccttcgt
     5881 gcgcgaccgg gcgacgtccc ggtatttcga tccggcccgc atgcacaccg tccaccacga
     5941 agggaggttc ttcaaggtag tcggcgccct caacatcgag cgcccgccgc agggccatcc
     6001 cgtgatcagc caggccggcg cctccgatac gggccgcgag ctggccgcgc gcacggccga
     6061 ggtcgtgttc gcgagtgcgg ctgctcccga gcccgcgaaa gccgcctatg atgacctgaa
     6121 gggccgcatg gccaagtatg gccgcgctcc cgagaccctg aaggttctcg cgggcctccc
     6181 cgtcgtcatc ggtcaatccc gagccgaggc cgaggagcgc ttcgagcgcc tgcagggcct
     6241 gctccacccc gacgtcggcc tcttccgcct cgccatggac ctcgaggtgg acctcaccgg
     6301 cctgcctctg gacgagccgg tccccgagag ccggctgccg aaaggcgcaa acttccacaa
     6361 ggctttcttc gacggcatca tggcctcgat ccgcgacgag aagcctacgc tgcgccagct
     6421 ctacatgcgc tacgagcgcg gccggaagac catcaagggc acgccgaagg acatcgccga
     6481 catcatggag cactggctgc actcaggcgc ttgcgacggc ttcatgctgc agttccacac
     6541 gcttccgcag agcctcgacg agttcgtcca cctcgtcgtg cccgagctgc agcgccgcgg
     6601 catttaccgt cgcgactatg cggggccgac cttgcgagac cacctgggcc tcgcccggcc
     6661 tgcaaaccca aacaccgtaa ggcactgagc ttggcggtcc tctttgcgca agtgctggtg
     6721 gcaacgacag ggctcgccca tgccctcgcc tcctggcagt acttcgcccg catttcgagc
     6781 cgcggctgct ggatctggcc gctcgcctgg gcgatcggca ccctcgcgag cctcgtcctg
     6841 atgcagagcg cgccgctggc gggcttcacg gccttcctcg ccctnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnccgcgc ctcgacgaca ctcgaatggg tcgcggagaa ccgcctccag
     6961 gcgacgggcc ggatcatcgg caacgaggtc gcgatcacaa acgtgcgcaa cttcgattgg
     7021 atcgacaagc ggacattcga ggaaaagtgg gaggcgcgac agtacgatat cgagacgctt
     7081 caggcgatcg atgtgttcgt ttcctcgtgg ggcaacccgc gcattgccca tatcatggtg
     7141 agcttcgact ttgcgggcgg gccgccgctc tgcgtatccg tcgagacccg ccgcgaggcc
     7201 ggcgagcgct ggtcggctct cgccggtttc atgaagagct acgagctgat cattatcgcg
     7261 ggcgatgagc gcgatctcgt gcgaagccgt gtcaatgtgc gcggcgagga cgtgcgcctc
     7321 tatcggatct acacgacgcc cgagatgcgg cggaaaatcc tggccgggac catcgcccag
     7381 atgaaccggc tttcggcgcg cccgcgcttc tacaacacca tcttccacaa ctgcactatc
     7441 gagatcgcgc ggatcgtgtg ggctgccggc caccgctttc ccttcgattg gcgcttgctc
     7501 gtctcgggct acgtggccga gtatctctac gacgtcggtc tactcgaccg aacgctgccg
     7561 tttgccggcc tgaagacatc ggccgacatc acgcagcgct cacgcactgc tgaccgcgca
     7621 ccggactact cgcagcgcat tcgcgcgggc ctccgtgacc cgacccgcga tatcgccgtc
     7681 acggcctcgc ctgtctctcc agcacgtcca caagtcggag cccgtccatg acctcgccga
     7741 gatcgcttcc cccgagccac cacttgcccc agcgtcccga gtggcttgcg aaggtcgtcg
     7801 aggagatcat cgagcccgac ctgccgatca tcgatcccca ccaccacctc tgggagcgtg
     7861 agggcaaccg ctacctcctc gacgacatcc tcgaggatac ggcgagcggc caccgcatcg
     7921 cctcgaccgt gttcatggaa tgccgcgcca tgtaccgcca gggcggcgac cccgagttgg
     7981 cctgcctcgg cgagacggag ttcgtgaacg gcatcgccgc catgagcgcc tcggggagct
     8041 acggcccgac gcgcgtcgcc gaggccatca tcggccgcgt gaacctgatg ctcggggccg
     8101 gcgcgcggcg catcctcgaa gctcagatgg cggtcgcggg cggacgcttc cgcggcgtgc
     8161 gctacatcac cgtctcgcat cccgatcccg cggcgcgcgg cgcgagcacc aatcaggagc
     8221 cgggcatcct gcgcgatccg cgcttccggg ctagttttgc cgagctcgcc cccctgggcc
     8281 tcacgttcga tgcctggatg taccacacgc agctcgacga actcgccgat ctcgcccgca
     8341 catatcccgc gacgacgatc atcgtcgatc atgtcggcgg accgcttggc ctcggcccct
     8401 atcaaggcaa gcgcgccgag gtgatggcgg agtggacaaa atcactgaag ggcctcgcct
     8461 cgtgtccgaa tgtctacatg aagcttggcg gcctcggcat gcgctttccg ggcttcgact
     8521 tccacgagcg ggagatgccg ccgacatcga agcagctcgc cgaggcctgg agcccctacc
     8581 ttgagacctg catctccgtc ttcggcgcaa gccggtgcat gttcgagagc aatttcccgg
     8641 tcgacaagcc gacatgcagc taccaggtgc tctggaacac gttcaagcgc gtcgcagcca
     8701 aggccactgc cgaagacaag cgcgccctat tctcaggcac cgccgcgaag gtctaccggc
     8761 tgcaacccgc tgtgtgacac ggttcagcca ctgccgtcat cgtccggggg tgtagcaatg
     8821 gccgccgggt ggtgcgctgc gatgatctcg agccagcggg caggcggtgc gtcgcgtctg
     8881 aggcggttga aatcggcctt cacgtggact gccttctcga ggaggccatg gaaggcgtcg
     8941 cggtcgacct cgaccgtgcc gaactgtcgc aggtgatcgg tgacgaattg cgtgtcgagc
     9001 agcgtgaagc cgccatggat gaggcgcgct gcaaggtgca cgagcgcaac cttgctcgca
     9061 tcgcgctcgg tgctgaacat gctttcgcca aagaaggcac caccgagcgc cacaccgtaa
     9121 agtccgccga ccagctggcc gcccgaccag gtctcgaccg tatggcagtg gccgagatca
     9181 tagagcgcgc cgtagagatg gcgaatgcga tcgttgatcc aggtcgtgcg tcgaccaggg
     9241 cgggaggcag cgcagccatc cataacacct cggaagtcgt aatcgatgcg gacctcgaag
     9301 gtgcccgctt tcacggtccg cgcgaggcgc ttcggcacat gaacgtgatc gagcggcagg
     9361 atgccgcgat gctgcggctc gatccagtag agggccggat catcggcgct ttccgccata
     9421 gggaagatgc cgcacgcata ggccttgagc aggacctgcg gcgtgatctc gatggcgatg
     9481 tcttcgcgcg atgccatagt tgcctgtgaa cgcgtttcga caacactact gcttcgcgac
     9541 gttgctggca aaaaattgtc tgaagattgt ccgcgcccgg tcaaaccgac cgcgaggcca
     9601 gattaaggcc ggccgtcgcc tctactggcg acgaccggcc cagaacgcga caactcccga
     9661 tcagcgcgtt gaagcggtcc gacgcggcgc gctcccggcg tccatcgcga cattggcggg
     9721 cagccggctc acgacatgaa cggttgtgcc gacgccgatg cggtccgcga ggtcgacgac
     9781 gtggccgttc atcatgcgga agcacccgga cgacgcggcg cggccgatcg aggccgcatc
     9841 gttcgtgcca tggatgcgat agagcgagtt gccgaggtag agcgccttgg caccgagagg
     9901 attgcggatg ccgcccgtca tcttctgcgg caggcgcgga tcacgctcgc gcatctcggc
     9961 cggcggatgc cagtcgggcc acgaggcgac gcgcgagatg cgctcggtgc cggtccaggt
    10021 gaagccctcg cgaccgaccg agatcggata gcggtaggcg gtcgtcgacg acgtgacgag
    10081 gtagagcgcg cggccgctcg tatcgatgac gacgcttccg gaggcataag agctgttgaa
    10141 ggcgaccgag gccggaccca
//
LOCUS       DAORNH010000157        10157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4903_length_10157_cov_2.466838, whole genome shotgun sequence.
ACCESSION   DAORNH010000157 DAORNH010000000
VERSION     DAORNH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10157
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4903_length_10157_cov_2.466838"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK264_15340"
     CDS             <1..322
                     /locus_tag="PK264_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179482.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HRD77282.1"
                     /translation="GMEVVYEGIRLTPAQIVRAALEESVHVVGLSILSGSHLPLVGAV
                     VAGMRKEGLAEIPVVVGGIIPPEDAMRLKADGVAAVYTPKDFDLTAIMRDIARLAAPG
                     ARAA"
     assembly_gap    383..587
                     /estimated_length=205
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<588..791)
                     /locus_tag="PK264_15345"
     CDS             complement(<588..791)
                     /locus_tag="PK264_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77283.1"
                     /translation="MFRVLLAVHGTLFLALLASGCASQTADSPPPLLSLVTPSAPAPP
                     ARETLVPVALAASQLEIVKAGLRP"
     gene            complement(836..2026)
                     /locus_tag="PK264_15350"
     CDS             complement(836..2026)
                     /locus_tag="PK264_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD77284.1"
                     /translation="MSNEPRRAGRHFLQIPGPSPVPDRILKAMAMPVIDHRGPDFAKL
                     GHRVLDGIKGIFKTKGPVIIYPASGTGAWEAALVNTLSPGDHVLMYETGHFATLWKNM
                     ALKLGVVPEFIASDWRSGADAAAIEARLREDRTHAIKAVCVVHNETSTGATSRIDEVR
                     QAIDRAGHPALLMVDTISSLASVDYRHDEWGVDVTVSGSQKGLMLPPGISFNAISPKA
                     IAAAKTAKLPKLFWSWDEMLPHNARGYFPYTPATNLLYGLAEAIDMLNEEGLDRVFSR
                     HARHGEATRRAVRAWGLEVLCRDEKYHSPVLTGVMMPTGHDADAYRKVVLDHFDLSLG
                     TGLSKVQGKVFRIGHLGDTNDLTILATLAGCEMGLALAGVPHKAEGVAAAMSYLRETA
                     RPTG"
     gene            2150..5080
                     /locus_tag="PK264_15355"
     CDS             2150..5080
                     /locus_tag="PK264_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD77285.1"
                     /translation="MTEAIGVNHALAERLRRETTAEVLVDAFSRGRYATDASIYQIMP
                     LGVIVPRTLEDVRTALAIAREEGVSVLPRGGGTSQSGQTVGRSLVIDYTKHLNRIVSA
                     DKDEATCVVEPGIVLDDLNRQLRASGLFFPVDVSTSARATIGGMTGNNSCGTRSLRYG
                     IMRDNVMAIDAVLPDGTPMRFDDGAESLVDGVAGRLASDLLALGTREADHIRTAFPTV
                     SRRVGGYLIDALLPSNRPVNLATLLCGAEGTLALSERITLKLSPVTRNRALGICHFPT
                     FRRAMEAAQHIVKLAPVAVEVVDRTLIELARDIAMFRPVMDAHVRGKPDALLLVEFAE
                     PEQAENVRRLKQLAELMGDLGTPDAVVEITDGLEQAAVWGVRAAGLNIMMSMKSDGKP
                     VSFIEDCAVPLENLADYTERLTEVFRRHGTTGTWYAHASVGCLHVRPVLNLKLKKDAA
                     TMRAIAEEAFALVKAYKGSHSGEHGDGLVRSEFHELMYGRRTVELFEEVKDRFDPKGL
                     MNPGKIVRPPRMNDRTLFRYAPTYAVPELRTALDWTPHGYAGAGGGLQGAVEMCNNNG
                     ECRKLVGGVMCPSYRATRNERDVTRGRANALRLAISGQLGPDAMASDEMLEAMKLCVS
                     CKACRRECPTGVDMAKMKIEVLAARHRRTGHVSLRDRLVAYLPRYAPSAARLAPLMNA
                     RNWLPGVAKLAERVTGFSAQRKLPVWRGKLFRLDAPVGEARAGEVALFADTFNTYFEP
                     ESLWESVEVLIALGYRVHPLSAADSRRPVCCGRTFLSAGLVDEARTELRRMLEAARPL
                     LDKGIAIVGLEPSCVLTFRDEATMLGMGDEVKRLAGATRLLDEFIAGEAAAGRIKGPI
                     GRREGRILLHGHCHQKSFGTMEATARTLRLIEGLEVETIETSCCGMAGAFGYGADTYN
                     VSISMAEASLLPAIRKAPEKDVVASGFSCRHQISDGTGRTPLHLARILAEAIKGGAAG
                     VAPP"
     gene            complement(5046..5570)
                     /locus_tag="PK264_15360"
     CDS             complement(5046..5570)
                     /locus_tag="PK264_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5289..5291),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HRD77286.1"
                     /translation="MTIRVLDPTNEATPAAIALAPRLAKLEGLTVGFISNGKEGTKGY
                     FSHLARMLKDEYGVRDVVLRVKSNYSAPADLHIVEEISQWHAAVSGLGDUGSCSSCSL
                     HDAIAAERLGVPAIGVMTTNFVSAARLMSRVLGMPDYAFAVIGHPVSSASDAELEAYA
                     RATVKEVPRLLLRP"
     gene            complement(5673..6461)
                     /locus_tag="PK264_15365"
     CDS             complement(5673..6461)
                     /locus_tag="PK264_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77287.1"
                     /translation="MAGLTPAEAYALLEPGSRKGNTAIKLTLMWLLSRRVIEIRKSAV
                     PGIFGKIRERDALFVREPLPADCPPYLRRLLAIIDNPSGVRVDRIPLMCGYVYGSQSH
                     SFLREDVSRALIAKGFITRTPRRALGIFPYEQYRRTKAGDQLASEINRTIVGLKSIPD
                     MLERNPRQAAALAFAAGGLIFLVPELVPHFTALAHAARRADETGGDSGVSVPSSSAAS
                     SDDDPWQQVDLDTFEALETTFSSFSEGFDSGGGDGGDGGGGGGD"
     gene            complement(6571..7038)
                     /locus_tag="PK264_15370"
     CDS             complement(6571..7038)
                     /locus_tag="PK264_15370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD77288.1"
                     /translation="MTDQPGAIDLSARQSFAFFTPVPIRFCDTDKMGHVNNCAITTFI
                     ESGRCDFVYGLIKAAGAAAEHVDFILARLAVDFRRELHYPGTVEVGTRLTRLGGKSVS
                     SGYGVFIGDTCYATAECVNVFYDPVGRRSMEPPPALRSLLERERATPGAVVRS"
     gene            7368..8543
                     /locus_tag="PK264_15375"
     CDS             7368..8543
                     /locus_tag="PK264_15375"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD77289.1"
                     /translation="MSQPKPLAGMTVIDISQGIAGPTCGAMFAEHGASVIKIEPPEGD
                     WVRKLGTQVAGTSTPGIVYNRGKRSIALDLKSPKAIAITKTLAKNCTVFIESGRPGAI
                     ERLGLGFKDIAAISPDVIYISVSGFGHVGPYRDRPMVDTVGQAWSGLMTRALGRDGSP
                     TRLTVPLIDVVTGFLSFQAATMALWGKKSGSGPKHIDASLMQAVATMQAPYIMEWAHV
                     GRAAGQLNPPAGIYRTKDRWIAVTLVTEAQYAGICRALGRPELASDPRFLTFAKRKEN
                     EAALREIIEVEVAKRPSTEWVAAFTKEGALAEPVATYGDWLADPHVKAADAAPPFEVA
                     PGVTLPLPRLPGQPPFNAKAPQVGENTRAVLAAAGLGPNDIEALIREGVAAEAGGRT"
     gene            8540..9031
                     /locus_tag="PK264_15380"
     CDS             8540..9031
                     /locus_tag="PK264_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HRD77290.1"
                     /translation="MSASTFDPAAHRLVPAQRWFEDFEVGESFALPSRTVTEAQFLAF
                     QAASGDNHPIHYDRPFCRRHGHKDLLAHGFQVLILTAAGAGVFPHMVEESLVAFIEQS
                     SRFLAPVYAGDTLYPRLTVAELKAQRTTGVLTLTSTIHNQDGKLVLEGRQVYLVRRRK
                     GDA"
     gene            9117..>10157
                     /locus_tag="PK264_15385"
     CDS             9117..>10157
                     /locus_tag="PK264_15385"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRD77291.1"
                     /translation="MASLDALLAQLKTLHPKLIDLSLDRSLALLEKLGRPQTRLPPVV
                     HVAGTNGKGSTTAYLASMLQAAGKRVHVYTSPHLVRFNERIAVAGPDGHTRPIDEDRL
                     AGLLLRVAKVNAGRPITFFEITTVAAFLAFSEIPADALILEVGLGGRFDATNVIDRPR
                     LSVITSISMYHAERLGDTVEKIAFEKAGILKPGVTAVVSQQPASVHEVIEECARALGS
                     PVVSFGRDFDAYAQGGRLVVQFADRLLDLPLPALVGRHQVINAGTAVAAASELGDLGL
                     TEAAIASGLTSAAWPARMQRLTHPPLLAALPAGSELWLDGGHNPGAGEMLAQTLADLE
                     EKSSKPLHLIVAM"
BASE COUNT         1637 a         3252 c         3416 g         1647 t
ORIGIN      
        1 cggcatggag gtcgtctacg agggtatccg gctgacgcca gcgcagatcg tgcgggcggc
       61 actcgaggag agcgtgcacg tcgtcggcct gtcgatcctc tcaggcagcc atctgccgct
      121 ggtcggcgcc gtcgtcgcgg gcatgcgcaa ggaaggcctc gccgagatcc ccgtcgtcgt
      181 cggcggcatc attccgcccg aggatgccat gcgtctcaag gctgacggcg tggcggcggt
      241 ctatacgccg aaggacttcg acctgacggc gatcatgcgt gatatcgcgc gtctcgcggc
      301 acccggcgcg cgtgcggcct gatcgacggt tgcacgggcg gaaacgtcag aagccgaggc
      361 cgtcctcggc gcacaccttg gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggg gcgcaagccg
      601 gccttcacga tctcgagctg gctcgccgct agcgccaccg gcacgagcgt ctcgcgggcc
      661 ggcggcgccg gggccgaggg cgtgacgagg ctcagcagcg gcggcgggct gtcggccgtt
      721 tgcgaggcgc aacccgatgc gagcagcgcc aaaaacagtg tgccatgaac ggccagcagt
      781 acccgaaaca catgcaactc ccgtttcgcc ggcgacaccc gccgacttgc cggggtcacc
      841 cggtcggtcg cgcggtttcc ctgaggtagc tcatggccgc ggcgaccccc tcggctttgt
      901 gcggcacgcc ggcaagcgcg aggcccattt cgcaaccggc cagcgtcgct aggatagtga
      961 ggtcgtttgt gtcgccgaga tggccgatgc ggaacacttt accttgcact ttggacaaac
     1021 cggtcccgag cgacagatcg aaatggtcga gcacgacctt tctgtaggcg tcggcatcgt
     1081 gcccggtggg catcatcacg cccgtcagca ctggcgaatg gtacttctcg tcgcggcaga
     1141 gcacctcgag cccccaggcg cggaccgcgc gccgcgtcgc ctcgccgtgg cgcgcatggc
     1201 gggaaaagac gcggtcgagc ccctcctcgt tcagcatgtc gatcgcctcg gcgaggccgt
     1261 agagcaggtt cgttgccggc gtgtagggga aatagccgcg cgcgttgtgt ggcagcatct
     1321 cgtcccacga ccagaagagc ttcggcagct tagcggtttt cgcggcggca atggctttcg
     1381 gcgagatcgc gttgaacgag atgccgggtg gcagcatcag ccccttctgg ctcccggaca
     1441 cggtcacgtc cacgccccac tcgtcgtggc ggtagtcgac cgaggcgaga gaggagatcg
     1501 tatcgaccat caggagggcc ggatggccgg cgcgatcgat cgcctgacgc acctcgtcga
     1561 tgcgcgacgt cgccccggta gatgtctcgt tgtggacgac gcagaccgcc ttgatcgcgt
     1621 gagtgcgatc ctcgcgaagc cgtgcctcga tcgccgcggc gtcagcgccc gagcgccagt
     1681 cggaggcgat gaattcgggc acgacgccga gtttcaaagc catgttcttc cagagtgtcg
     1741 cgaagtgccc ggtctcgtac atgagcacgt gatcgccggg cgagagcgtg ttgacgaggg
     1801 cggcctccca ggcgccggtg ccggacgcgg gatagatgat cacggggccc ttggtcttga
     1861 agatgccctt gatgccatcg agcacgcggt ggccgagctt ggcgaagtct gggccgcgat
     1921 ggtcgatcac cggcatcgcc atggccttca ggatgcgatc gggcaccggc gaagggcccg
     1981 ggatctggag gaaatgccgg ccggcgcggc ggggctcgtt ggacatgggg cttggctccc
     2041 tgagtctcgt gttgattggg gcttgcagcc cgcgaatgtg gcatgcaagg cgtaggttgc
     2101 gccctggcgg ggcgtcaagt gcaagtgtcg gctcaatggg cgcgagttca tgacagaagc
     2161 gattggcgtc aatcatgcac tggccgagcg gctccgccgc gagaccaccg ccgaagtgct
     2221 tgtcgacgcg ttctcgcgcg ggcgctatgc gacggacgcc tcgatttatc agatcatgcc
     2281 gctcggcgtg atcgtgccgc gcacgctcga ggatgtgcgc acggcgctcg cgatcgcacg
     2341 cgaggagggc gtttcggtcc tgccgcgcgg gggaggcacc tcgcagtccg ggcagaccgt
     2401 cggccgttcg ctcgtcatcg actacacgaa gcacctcaat cgcatcgtca gcgccgacaa
     2461 ggacgaggcg acatgcgtcg tcgagcccgg catcgtcctc gacgatctca accgtcagct
     2521 ccgcgcttcg ggcctcttct tcccggtcga tgtctcgacg tcggcccgcg ccaccattgg
     2581 cggcatgaca ggaaacaaca gctgcggcac gcgctcgctg cgctacggca tcatgcgcga
     2641 caacgtgatg gccatcgacg cggtgctccc cgacggcacg ccgatgcgct tcgatgacgg
     2701 cgccgagagc ctggtggatg gcgtcgccgg tcgtctcgcg tccgacctcc tcgcccttgg
     2761 cacgcgcgag gccgatcata tccgcaccgc cttcccgacc gtctcgcggc gtgtcggcgg
     2821 ctatctcatc gatgcgctcc tgccttcgaa ccggccggtc aacctcgcga cactgctctg
     2881 tggcgcggag gggacgctcg ctctaagcga gcgcatcacg ctgaagctct cgccggtcac
     2941 gcgcaaccgc gcgctcggca tctgccattt ccccaccttc cgccgcgcca tggaggcggc
     3001 gcagcacatc gtcaagctcg cgccggtcgc ggtcgaagtg gtcgaccgca cgctgatcga
     3061 gctcgcgcgc gacatcgcaa tgttccgccc ggtgatggat gcgcacgtgc gggggaagcc
     3121 cgacgcactg ctgctcgtgg agttcgccga gccggagcag gcggagaacg tgcggcgcct
     3181 gaagcaactc gcagagctga tgggcgatct cggcacgccc gatgctgtcg tcgaaatcac
     3241 ggacggcctc gagcaggcgg cggtatgggg tgtgcgcgct gccggcctca acatcatgat
     3301 gagcatgaag agcgacggga agcccgtctc cttcatcgag gactgcgcgg tgccgctcga
     3361 gaacctcgcc gactacacgg agcgcctgac cgaggtcttc aggcggcacg gcaccacagg
     3421 aacctggtat gcgcacgctt ccgtcggctg cctgcatgtg cgcccggtcc tgaacctgaa
     3481 gctcaagaag gatgcggcca caatgcgcgc gatcgccgag gaggcgttcg ccctcgtgaa
     3541 agcctacaag ggctcgcact cgggcgagca cggcgacggc ctcgtgcgct ccgagttcca
     3601 cgagctcatg tatggccggc gtacggtcga gcttttcgag gaggtgaagg accgcttcga
     3661 cccgaagggt ctcatgaacc cgggcaagat cgtccgcccg ccgcgcatga acgaccgcac
     3721 gctctttcgg tatgcgccga cctatgccgt tcccgagctc cgtaccgcgc tcgactggac
     3781 gccgcacggc tatgcaggcg ccggcggcgg cctgcagggc gccgtcgaga tgtgcaacaa
     3841 caacggcgag tgccgcaagc tcgtgggcgg cgtgatgtgc ccgagctaca gggcgacccg
     3901 caacgagcgc gacgtgacgc gcggacgcgc caatgctttg cgcctcgcga tctcgggcca
     3961 gctcggtccc gatgccatgg cgtcggacga gatgctcgag gcgatgaagc tctgtgtctc
     4021 gtgcaaggcc tgccgccgcg agtgtccgac cggcgtcgac atggcgaaga tgaagatcga
     4081 agtgctggcg gcacgccatc gccgcaccgg ccacgtctcg ctgcgcgacc ggctcgttgc
     4141 ctatctgccg cgctacgccc cgagcgcggc gcgactggct cccctgatga atgcgcgcaa
     4201 ctggctgccg ggcgttgcca agctcgccga gcgggtgacg ggtttttcag cgcagcgcaa
     4261 gctgcccgtt tggcgcggca agctcttccg tctcgacgcg cccgttggtg aggcgagggc
     4321 gggtgaggtt gcgctgtttg cggacacgtt caacacctac ttcgagcctg agagcctctg
     4381 ggaaagtgtc gaggtgctga tcgcgctcgg ctaccgggtg catccgctca gcgccgccga
     4441 cagccgccgg ccggtgtgct gcggccggac cttcctcagc gccggtctgg tcgatgaggc
     4501 gcgcacggag ctgcgtcgca tgctcgaggc cgcacggccg cttctcgaca agggcatcgc
     4561 gatcgtcggg ctggagccga gctgcgtgtt gacgttccgg gacgaggcga cgatgctcgg
     4621 gatgggtgat gaggtgaagc gtcttgccgg tgcgacgagg ctcctcgacg aattcatcgc
     4681 gggagaggcg gccgctggcc gcatcaaggg tccgatcggc cgccgtgagg ggcggatact
     4741 gctgcatggc cactgccacc agaagtcgtt cggcaccatg gaggcgacgg cgcgcacctt
     4801 gcggctgatc gaggggctcg aggtcgagac gatcgagacg agctgctgcg gcatggcggg
     4861 cgccttcggc tatggcgccg acacctacaa cgtttcgatt tccatggccg aggcgagcct
     4921 tttgccggcg atccggaagg caccggagaa ggacgtggtg gcatcagggt tctcctgccg
     4981 ccatcagatc agcgacggca cggggcgcac gcccttgcat ctcgcccgca ttctagcgga
     5041 agcaatcaag ggcggagcag caggcgtggc acctccttga cggttgcgcg ggcgtaggcc
     5101 tcgagctcgg cgtcgctcgc gctgctcact ggatggccga tcacggcgaa tgcgtagtcg
     5161 ggcatgccga ggacgcggct catcaggcgc gccgcggaga cgaagttcgt ggtcatgacg
     5221 ccaattgcgg ggacgccaag tcgttcggct gcgatcgcgt cgtgcaaact gcacgatgag
     5281 cagctccctc aatctccgag gccgctcacc gctgcatgcc actggctgat ctcttcgacg
     5341 atgtgcaggt ccgccggcgc gctgtagttg gatttgaccc gcagcacgac atcgcggacg
     5401 ccgtactcgt ccttgagcat ccgggccaga tgcgaaaagt agcccttggt gccttccttg
     5461 ccgttggaga tgaagccgac ggtcaggccc tcgagtttgg caaggcgcgg agcaagcgca
     5521 atggccgcgg gggtggcttc attggtcggg tcgaggacgc ggatggtcat gggcagggct
     5581 ccttcgctgc tcgcgccagg ataggcggct tcgcccgcct gctgcaacac gcccgctcgt
     5641 catggccgac cggcccggga cacaaggcgc gatcagtcgc cgccaccgcc tccgccgtcg
     5701 cctccatcgc cgccgccgct gtcgaagccc tccgagaacg acgagaacgt ggtttcgagc
     5761 gcctcgaacg tgtcgaggtc gacctgctgc cagggatcgt cgtccgaaga cgcggcactc
     5821 gaactcggta ccgaaacacc gctatcacct cccgtctcat cggcgcggcg ggcagcatgg
     5881 gcgagtgctg tgaagtgcgg cacgagttcc gggacaagga agatcagccc accagccgcg
     5941 aatgcgagag cggctgcctg gcgcgggttg cgctcgagca tgtcggggat cgacttcaga
     6001 ccgacgatgg tgcgattgat ttcactggca agctggtcgc cggccttcgt tcgccggtac
     6061 tgctcgtagg ggaagatgcc gagggcacgg cgtggcgtcc gtgtgatgaa gcccttggcg
     6121 atcagtgcgc ggctcacgtc ctcgcggagg aacgagtgcg actggctgcc gtacacgtat
     6181 ccgcacatca gcgggatgcg gtccacgcga acgccgctcg gattgtcgat gatggcgagc
     6241 aggcggcgga gatagggtgg gcagtccgcc ggaagaggtt cacggacgaa caatgcgtcg
     6301 cgctcacgaa ttttgccgaa gatccccggt acggccgact tgcggatctc gatcacacgt
     6361 cgcgacagca gccacatcaa agtgagcttg atcgccgtgt tgcccttgcg gctgcccggc
     6421 tcgagaagcg catacgcctc ggccggggtg agacctgcca tcaggcctcg tggttccaat
     6481 atcatcgtca tatctgagca ttgtgtagca cagatcgaca atcggcgcca ggcagcgcag
     6541 ccgggacggg taacggagtg ttacccgcac tcagctccgc acgacggcgc cgggcgtcgc
     6601 ccgctcgcgc tcgaggagcg accggagcgc tggcggcggc tccatggagc ggcggccgac
     6661 gggatcatag aagacgttca cgcactcggc cgtcgcatag caggtgtcgc cgatgaacac
     6721 gccgtagccg ctgctcaccg atttgccgcc gagccgcgtg agccgcgtgc cgacctcgac
     6781 cgtccccggg taatgcaact cgcgacggaa gtcgaccgcg aggcgggcga ggatgaagtc
     6841 aacgtgctcg gctgcagcgc cggcggcctt gattaggccg tagacgaagt cgcagcgccc
     6901 ggattcgatg aaggtcgtga tggcgcagtt gttgacgtgg cccatcttgt ccgtatcgca
     6961 gaagcggatc ggcacaggcg tgaagaatgc gaagctctga cgcgcgctga gatcgatcgc
     7021 gccgggctgg tcagtcatgt ccgatccgtc ctatcgttgg ctctatctaa gcccgaactc
     7081 ttcaccaccg cgttgttcgc gatcgcacga ggcgggctcg atccggcaga aggatggcga
     7141 aaagcgtagc tctggcttac gcttgagcca tccgacgcac cgggcggccc gcatcgtgcg
     7201 acccggaggg cggggcaaat ttgacgattg acggcgtcga accgcttgac cgatgtccct
     7261 gcatcgctct gcgcgtctct cctggtcaat cgtcaaattt gcctccgtcg cgaccacgtg
     7321 ctggtgaaga gttcgggcta gctcggctct taacagggaa gcggcacatg agccaaccga
     7381 agccactcgc cggcatgacg gtcatcgaca tttcgcaggg gatcgccgga ccgacgtgcg
     7441 gggcgatgtt cgccgagcac ggggcgagcg tgatcaagat cgaaccaccc gagggggact
     7501 gggtgaggaa gctcggcacg caggtcgccg gcacctcgac gccggggatc gtctacaacc
     7561 gcggcaagcg ctcgatcgcg ctcgacctca agtcgccgaa ggcgatcgcg atcacgaaga
     7621 cgctcgcgaa gaattgcacg gtcttcatcg agagcgggcg gccgggcgcg atcgagcggc
     7681 tcggtctcgg cttcaaggat atcgcggcga tctccccgga cgtgatctac atttcggtgt
     7741 caggcttcgg ccacgtcggc ccctaccgcg accggccgat ggtggatacc gttggccagg
     7801 cctggtcggg tctcatgacg cgcgcgctcg gccgcgatgg cagcccgacg cgcctcactg
     7861 tgccgctgat cgacgtcgtg acgggcttcc tgtcgttcca ggcggcgacc atggcgctct
     7921 ggggaaagaa gtcggggagt ggaccgaagc atatcgacgc gagcctgatg caggcggtcg
     7981 ccaccatgca ggcgccctac atcatggagt gggcgcacgt cggacgcgcg gcgggacagc
     8041 tcaatccgcc cgcgggcatc tatcggacca aggaccgctg gatcgccgtg acgctcgtca
     8101 cggaagcgca gtatgccggc atatgcaggg cgctcggcag acccgagctc gcgagcgacc
     8161 cgcgcttcct caccttcgcc aagcgcaagg agaacgaggc ggcactgcgc gagatcatcg
     8221 aagtcgaggt cgccaagcgc ccgtctactg aatgggtggc ggcctttacg aaggaaggcg
     8281 cgctcgctga gccggtcgcg acgtacggcg actggctcgc cgacccgcac gtgaaggcgg
     8341 ccgatgcagc gccgcccttc gaggtagcgc cgggtgtgac gctgccgttg ccgcgtctgc
     8401 cgggccagcc ccccttcaac gccaaggcac cgcaggtcgg cgagaacacg cgggccgtac
     8461 tcgcggcagc ggggctcggg ccgaacgaca tcgaggcact catcagggag ggtgtcgcag
     8521 ccgaagcggg agggcgcacg tgagcgcgtc gaccttcgat ccagcagcgc accgcctcgt
     8581 ccccgcgcaa cgctggttcg aggacttcga ggtaggcgag agcttcgcgc tgccgtcgcg
     8641 cacggtgacg gaggcacagt tcttggcctt ccaggcggcc tccggcgaca atcacccgat
     8701 ccactacgat cgcccgtttt gccgtcggca cggccacaag gacctgctcg cgcacggctt
     8761 ccaggtgctg atcctgacgg ctgcgggggc gggagtcttc ccgcacatgg tggaggagag
     8821 cctcgtcgcc ttcatcgaac agtcctcgcg tttcctggcg ccggtctatg ccggtgatac
     8881 gctttatccg cgcttgaccg tcgccgagct gaaggcgcag cggacgacgg gcgtgctcac
     8941 gctgacgtcg accatccaca accaggacgg caagctcgtg ctcgagggcc gacaggtcta
     9001 tctcgtgagg cggcggaagg gcgatgcttg acttttgcca ccggtgcgtg cggatcgcgg
     9061 tcgtagcaaa cggtccgcgc tgagacggca cccggccggc atgaaggctc ccgatcatgg
     9121 catcactcga cgcgctcctc gcacagctga agacgctgca cccgaagctc atcgatctct
     9181 cgctcgatcg ctcgctcgcg ttgctcgaga agctcggacg gccgcagacg cgcctgccac
     9241 cggtggttca cgttgccggc acaaacggca aaggctcgac aacggcctat ctcgcgtcga
     9301 tgctgcaggc ggcggggaag cgcgtccacg tctacacgtc gccccacctg gtgcgcttca
     9361 acgagcggat tgcggttgcc ggccccgatg gtcataccag accgatcgac gaggatcggc
     9421 tggccgggct cctgctgcgc gtcgccaagg tcaatgccgg ccggccgatc acgttcttcg
     9481 agatcacgac ggttgcggcg ttcctcgcct tctccgagat cccggccgac gcgctgatac
     9541 tcgaggtggg gctcggtggc cgtttcgatg cgaccaacgt gatcgaccgg ccgcggctct
     9601 cggtcatcac ctcgatctcg atgtatcatg ccgagcgact cggcgatacg gtcgagaaga
     9661 tcgccttcga gaaggccggc atcctgaagc cgggcgtgac ggccgtcgtg tcgcagcagc
     9721 ccgcgagcgt ccacgaggtc atcgaggagt gcgcgcgggc gctcggctcg cctgtcgtga
     9781 gcttcgggcg ggacttcgac gcctatgccc agggcggtcg tctcgtcgtg cagttcgcgg
     9841 accggctcct cgatctgccg ctgcccgccc ttgtcggccg gcaccaggtc atcaacgcgg
     9901 ggacggccgt tgccgcggcg agcgaactcg gcgatctcgg cttgaccgag gcggcgatcg
     9961 cgagcggctt gacgagcgcc gcctggcctg cccgcatgca gcggctcacc cacccgccgc
    10021 tgttggcggc cctgcccgcg ggctcggagc tgtggctcga cggcggccac aatccgggtg
    10081 ccggcgaaat gctcgcgcag acgctcgccg acctcgagga aaagagctcg aagcctttgc
    10141 atctgatcgt cgccatg
//
LOCUS       DAORNH010000158        10151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4908_length_10151_cov_2.484945, whole genome shotgun sequence.
ACCESSION   DAORNH010000158 DAORNH010000000
VERSION     DAORNH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4908_length_10151_cov_2.484945"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>226
                     /locus_tag="PK264_15390"
     CDS             <1..>226
                     /locus_tag="PK264_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRD77292.1"
                     /translation="SDLSALGINMNCVPVLDVPVAGAHDIIGDRAYGESVAPIVALGR
                     AVADGMMAGGVVPVMKHIPGHGRATADSHLA"
     assembly_gap    227..344
                     /estimated_length=118
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <345..683
                     /locus_tag="PK264_15395"
     CDS             <345..683
                     /locus_tag="PK264_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRD77293.1"
                     /translation="QSNVAVGGQFQPRTGYIGFDGLLMSDDLSMKALSGPFAARARAV
                     LDAGSDLALHCNGALDEMKAVAAAAPRIAGACEARLERALAVTRAPRAFDIVAAESAL
                     ATIMALNSVA"
     gene            796..1605
                     /locus_tag="PK264_15400"
     CDS             796..1605
                     /locus_tag="PK264_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HRD77294.1"
                     /translation="MTPDFNLEPVGEHDEDAGESLTVSLDGFEGPLDLLLALARTQKV
                     DLSALSMSALADQYLSFIAEARAVRLELAGDYLVMGAWLAFLKSRLLLPKEEVCDDVP
                     SGEELAARLAFRLMRLEAMRNAGAQLLTRKQLGRDVFPRGMQEPMRVVRERQYVAEIY
                     DLLKAYADQRKRTIKVQHVVKARTVWSIKEARRHLERLVGTAAGSWVQLDMFLAEYLP
                     APELGRTALASSFGASLELAREGRIELRQEQHFAPIYMRRREDGAAWERIG"
     gene            1681..2535
                     /gene="scpB"
                     /locus_tag="PK264_15405"
     CDS             1681..2535
                     /gene="scpB"
                     /locus_tag="PK264_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRD77295.1"
                     /translation="MNPPRLNVAHRDDYDRTDEHRADDAECPSEVPAAVRGPAGQHGL
                     PANIAVLPSADRREQLRILEALLFAAREPQDEASLARYFKPGQDVLALLEELKGAYAG
                     RGINLVRVAGKWIFRTAEDLSYLLERHAVEQRRLSRAALETLAIIAYHQPVTRAEIEE
                     IRGVQTSKGTLDVLLETGWVKPRGRRRAPGKPLTYGTTDRFLEQFGLDNLKELPGLAD
                     LKGAGLLDGTLPADFKVPSPGDLAAVLPDELPLDDTDGAEPETQSELDLDLPSDEEAD
                     NGPGGAAT"
     gene            2583..>2881
                     /locus_tag="PK264_15410"
     CDS             2583..>2881
                     /locus_tag="PK264_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77296.1"
                     /translation="MTWFWQRRRSLEAAQLQGVGERSRASAAFAGSLAFEGVERRYGD
                     KLALAGVSIEVGAGQILCLLGPSGCGKTTLLRIASGVEKPSAGRLLINGREIAGP"
     assembly_gap    2882..3065
                     /estimated_length=184
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3066..>3587
                     /locus_tag="PK264_15415"
     CDS             <3066..>3587
                     /locus_tag="PK264_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD77297.1"
                     /translation="LSGGEQQRVALARALVPRPAVVLMDEPFSGLDIQLRESMQEETL
                     ALLRETRATSLVVTHHAEEAMRLGDRIAIMRAGRIIQHGRANELYRDPADLFVARLFS
                     EINEVPWKVAGGALRTPIGVFSVPGLAEGEGAVLCIRQRAIRIVPAGKGQPGRVLHTR
                     FLGDAAESEEAVA"
     assembly_gap    3588..3597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3884..3993
                     /estimated_length=110
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3994..4213
                     /locus_tag="PK264_15420"
     CDS             <3994..4213
                     /locus_tag="PK264_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HRD77298.1"
                     /translation="RGRRAKKPEYEVVEAVAPEPAVEGEEALETVEGDAEPVAAADDE
                     TFLEEEEDQGSDVSGIISGGVAEEGEEQ"
     gene            4338..4646
                     /locus_tag="PK264_15425"
     CDS             4338..4646
                     /locus_tag="PK264_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77299.1"
                     /translation="MRLRGHLLPGVVGSTALVSALLAALPAAAQTPQPSVPGITVVPP
                     PAPRPARPSGDGQVAPPSRPSDQPDGAAPRRNEPDDDEGATSSPGGCPFQKKKLELIV
                     "
     gene            4713..5306
                     /locus_tag="PK264_15430"
     CDS             4713..5306
                     /locus_tag="PK264_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HRD77300.1"
                     /translation="MLSGRRLDLLDPSPADIEIDDIAHGLARVARWNGQTLGPHAFSV
                     AQHSLIVEEIVGALHQGEVSPSVRLAALLHDAPEYVIGDLISPFKAAIGLDYKAFEVR
                     LLGAIHERYGLPRVLAETDARMIKEADRIAAYFEATRLAGFADAEAREFFGTPPRLDR
                     ELSLALSMLSASSPTLAGAQFLERFAALTDAITRQQI"
     gene            5455..5688
                     /locus_tag="PK264_15435"
                     /pseudo
     CDS             5455..5688
                     /locus_tag="PK264_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
     gene            5804..6700
                     /locus_tag="PK264_15440"
     CDS             5804..6700
                     /locus_tag="PK264_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRD77301.1"
                     /translation="MISITAKDKLRQQLGKNMVMSRGDISFSSEKADKCTMAENGITN
                     VLELVGLPLPDMYSAELSLFVLEAGVKLLEREKPDLMYLSLTDYIQHKYAPDEGEALR
                     YMKNCDELFGKLDRLGAIVALTADHGMNDKTRDDGSYRVVWLQDLLDKAFGRGKTTVI
                     CPITDYFVAHHGALGGFVRVYSNDKAVTAKAIMEVARAAKGIEFVCDKATAVETFGLP
                     ADREGDVVVIGDVGTCIGTREQEHDLKGLEGHRLRSHGSTTEARVPLIINTPLNAEYA
                     AKAKRGGIRNFNVFEYAVNGTA"
     gene            complement(6707..7765)
                     /locus_tag="PK264_15445"
     CDS             complement(6707..7765)
                     /locus_tag="PK264_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRD77302.1"
                     /translation="MTTASVIEDRPWLDTLARIEALEDGAFDAALVCEMQRAAAEQGG
                     LAPIRFSTPTFKEYATSELKGCSKNSFPAFSVTAGACALDCDHCQAKILEPMIPATSP
                     EMLERKVRDLILLQDLQGFLLSGGSNRRNEIKYEKYLPVIERLKRDHPHLRIAIHSAL
                     LDRPRAKAMESAGVDTAMMDVIGAEETIREVYHLDRRVADFEETLAALCSTTMEIVPH
                     IVIGLHYGRIVGEAAALDICARQPIHSLVLVVVMPFYARPGTFVTPATVDVGRIFLEA
                     RRRLPGRPVLLGCARPAGMHKRVTDAYAVMAGLDGIAFPADGAVALSEAIGRPYEQQN
                     ACCSIKVGGSISAGGGCR"
     gene            complement(7762..>7863)
                     /locus_tag="PK264_15450"
     CDS             complement(7762..>7863)
                     /locus_tag="PK264_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77303.1"
                     /translation="GPPIWDVVGSYLKRKGTVKAGNVGEVKVVRSRT"
     assembly_gap    7864..7892
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7893..9483)
                     /gene="soxB"
                     /locus_tag="PK264_15455"
     CDS             complement(<7893..9483)
                     /gene="soxB"
                     /locus_tag="PK264_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HRD77304.1"
                     /translation="MLSRRDFIQVALAASALTAGSGSLARLSAQQKIGEADLLKFDAK
                     GQVTLLHMTDCHGQLLPHYFREPSVNLGVGEVYGEPPHLTGQEFLAKFGIPAGSHQCY
                     MLSDDDFSALAKTYGRVGGMDRMATLVKAIRAERGADRTLFLDGGDTLHGSYTVMKSN
                     GADMAAVLDMLGAEATTGHWEFTRGEKRVGELFGGSEKAGSVKASFLAANIRETEFDE
                     PAFKAVKHVVKGGVKIAIIGQAFPFTPIANPRWMMPKWSFGIREKDVRKQVTAARAAG
                     AELVILLSHNGFDVDRKLAARVSGIDVILTAHTHDALPVPIKVGRTLLIASGSHTKFL
                     SRLDIEMRDGRMADYRYALIPVLADAIRPDPEMAAHIAKVREPHAAMLATELARTEDL
                     LYRRGNFNGTLDNVICDAMLAERDAEICLSPGFRWGTSVLPGEGITWDTVYNATAISY
                     PKCYRLPMKGEMLKAILEDVADNLFNKDPYYQQGGDMVRVGGMGYTIDVDKPVGSRIS
                     GMTHLKTGTPIEPGRDYTVAGW"
     gene            complement(9638..>10151)
                     /locus_tag="PK264_15460"
     CDS             complement(9638..>10151)
                     /locus_tag="PK264_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRD77305.1"
                     /translation="EEFEQLKRMAPGRRLKVALPGPITFAGFLPPRHGQRVKRIKEIS
                     TVPATLVIYEAPGRVAEALADLSAVLGSRPAAIARELTKRFEEVRRGTLAELAAGAAD
                     EPPQGEVVLVIAPPVAVEIGDEAIDAALRVKLADSSLRDAADAVAAELGVARRRAYEI
                     GLAIKRSRRD"
BASE COUNT         1590 a         3194 c         3264 g         1652 t
ORIGIN      
        1 tccgatctca gcgcgctcgg catcaacatg aactgcgtgc ccgtgctcga tgtgccggtg
       61 gctggcgctc acgacatcat cggcgaccgg gcttatggcg agagcgtcgc gccgatcgtt
      121 gctctcggcc gcgccgtcgc ggacggtatg atggccggcg gcgtcgttcc ggtgatgaag
      181 cacattcccg gacatggccg agcaacggcc gacagccatc tcgcgcnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaatcg aatgtggctg
      361 tcggcgggca gttccaaccg agaaccggct acatcggctt cgatggcctg ctcatgagcg
      421 acgatctgtc gatgaaggcg ctctccggcc cgttcgccgc gcgcgcccgc gcggtactgg
      481 acgcgggctc cgatctggca cttcactgca atggcgcgct cgacgagatg aaggcggtgg
      541 ccgcagccgc gccgaggatt gccggcgcgt gcgaggcgcg tctcgagcgg gctctggctg
      601 tgacgagggc gccacgggcg tttgacatag tggccgccga atcggcactt gccaccatca
      661 tggccctgaa ttcagtggca tgaatgccgg tgaatcagtg taattctgtg gaaaagtaaa
      721 cgacccgtta acgcgtatgc tggccgcctg cccatcgcgt tcttgctgca cgcgccctga
      781 tcgtctggga gcttcatgac gccggatttc aatctagagc cagtgggcga gcacgacgag
      841 gacgccggcg agagcctcac cgtgtcgctc gacggcttcg aggggccgct cgatctgctg
      901 cttgccttgg cgcggacgca gaaggtcgac ctgtcggcgc tctcgatgtc ggcgctcgcc
      961 gaccagtatc tctcattcat cgccgaggca cgcgccgtgc gcctcgagct tgccggcgac
     1021 tatctcgtga tgggcgcctg gctcgcattc cttaagtcgc gcctccttct ccccaaggaa
     1081 gaggtctgcg acgacgtgcc ttccggcgag gagttggcgg cgcggctcgc cttccgcctg
     1141 atgcggctcg aggcgatgcg taacgccggc gcccagcttc tgacccgcaa gcagctcggt
     1201 cgcgacgtct ttccgcgcgg catgcaggag ccgatgcgcg tcgtccgtga gcgccagtac
     1261 gtcgccgaga tctacgatct cctcaaggcc tatgccgacc agcgtaagcg cacgatcaag
     1321 gtgcagcacg tcgtcaaggc gcgcactgtc tggtccatca aggaggcgag gcgccacctg
     1381 gagcgtctcg tcggcaccgc ggccggcagc tgggtgcagc tcgacatgtt cctcgcggaa
     1441 tacctgccgg cgcccgaact cggccgcacg gcgctcgcga gctccttcgg ggcgtcgctc
     1501 gagctcgcgc gcgagggccg gatcgagctc cggcaggagc agcattttgc gccgatttac
     1561 atgcggcggc gtgaggacgg cgcggcgtgg gagcgaatcg gttaatattc tctttcgagt
     1621 cagtggattg tcggcgttgg agtgtcagtg aaaccaccgc gaataacccc gggggcctcc
     1681 atgaacccgc cacggctcaa cgtcgcccac cgcgacgact acgaccgaac tgacgagcac
     1741 cgcgccgatg acgccgaatg cccgtccgag gtgccggctg ctgtgcgggg accggcgggc
     1801 cagcacggcc tccctgcaaa tatcgccgtc ctgccctcgg ctgaccgccg cgagcagctg
     1861 cgcatcctcg aagccctgct gttcgccgcg cgcgagccgc aggatgaagc ctcgctcgcc
     1921 cgctacttca agccggggca ggatgtgctc gcacttctcg aggagctgaa gggcgcctat
     1981 gcggggcgcg gcatcaacct cgtgcgggtc gctggaaagt ggatttttcg gaccgcggag
     2041 gacctctcct atcttctgga gcgccatgcg gtggagcagc gccggctgtc gcgggcagcg
     2101 ctcgagaccc tcgccatcat cgcctatcat cagccggtga cgcgggccga gatcgaggaa
     2161 atccgcggtg tccagacctc gaaaggcacg ctcgacgtgc ttctcgaaac cggctgggtg
     2221 aagccgcgcg gtcgccgccg ggccccgggc aagccactga cctacggcac cacggatcgc
     2281 ttcctcgagc agttcggtct cgacaacctg aaggagctgc cgggcctcgc cgacctgaag
     2341 ggcgccggcc ttctcgatgg tacgctaccg gccgacttca aggtgccgag ccccggtgat
     2401 ctcgcggccg tactccccga cgagctgccg ctcgatgata ccgacggcgc cgagccggag
     2461 acccagtccg agctcgacct cgacttgccg agcgacgagg aagccgataa tggccccggt
     2521 ggcgccgcga cctgagcggc caaggggtgc tcaatgcctg cgttgcggcg taatgtgctg
     2581 atatgacctg gttctggcaa aggcgccgca gtctggaggc tgcccagctg cagggtgtgg
     2641 gtgagcgttc ccgggcgagt gctgcttttg ctgggagcct ggctttcgag ggtgttgagc
     2701 ggcgctacgg cgacaagctc gcgcttgccg gcgtgtcgat cgaggtcggc gccggacaaa
     2761 ttctctgcct cctcggcccg tcgggatgcg gcaagaccac gctgctgcgt atcgcctccg
     2821 gcgtcgagaa gccgtccgcc ggccgcctcc tcatcaatgg ccgcgagatt gccgggccgc
     2881 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnatctc tccggcggcg agcagcagcg cgtggcgctc gcccgggcgc tcgtcccgcg
     3121 accggccgtc gtgctcatgg acgagccgtt ctcgggcctc gacatccagc tccgcgaaag
     3181 catgcaggag gagacgctcg ctctcctgcg cgagacgcgg gcgacctcgc tcgtcgtcac
     3241 ccatcatgcc gaggaggcca tgcgtctcgg cgatcgcata gcgatcatgc gcgccggcag
     3301 gatcatccag cacggccgcg ccaacgagct ctaccgcgat cctgcggatc tttttgtcgc
     3361 gaggctcttc tcggagatca acgaggttcc ctggaaggtc gctggcggcg ccttgcggac
     3421 ccccatcggc gtcttctcgg tgccgggcct cgcagagggc gagggggcgg tgctctgcat
     3481 tcgccagcgg gccatccgca tcgtgccggc cggcaagggg cagccggggc gcgtgctgca
     3541 cacccgcttt ctcggcgatg cggccgagtc ggaggaggcg gtcgctgnnn nnnnnnnatg
     3601 cggccatgat ggagatcgcg gtcgagggct tcgagcgacc actgaaagca atggtcgagg
     3661 agggtgcggt tcgcgacaag ggcgcggatg tggccatcgc ggtcacgcca gaaagcgtgc
     3721 tggtcttccc cggagaagcg aaggagtagg tcgcgaatag ctcaagcgat gatgctgccg
     3781 cgaatgactt tgacagcctg ggtggtgcgt ggcaatgacc ccgcctaaga gattgatcga
     3841 ggcttgaccc atgtcgaaga aagcagcgcg cggcaccaag cgcnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagaggg cgccgcgcca agaagcctga
     4021 gtacgaggtc gtggaagccg tggcaccgga gcctgccgtc gaaggcgagg aggcgctcga
     4081 aaccgtcgag ggcgatgccg agcccgtggc ggcggcagac gacgagacct tcctcgagga
     4141 ggaggaggac cagggctccg acgtctcggg catcatcagc ggtggtgtcg ccgaggaagg
     4201 cgaggagcag taaggccggc gcgccgacct gattggcgct tcgagcccgc tccatgctat
     4261 gaagagcgac tctcgggagc ggggctcccg atatttcgct cgctcacgct tgcgggcatt
     4321 gccgaaaggg acgtctcatg cgcttgcgtg gacacctcct gccgggcgtg gtcggttcaa
     4381 cggctctcgt ttccgcgctg ctcgctgcat tgccggctgc ggcccagaca ccgcagccgt
     4441 cggttccagg cattaccgtt gttccacctc cggctcctcg gcccgcacgg ccgtctggcg
     4501 acggtcaggt cgcgccgccg agcaggccgt cggaccagcc tgacggcgca gcaccgcgcc
     4561 gcaacgagcc ggacgacgac gaaggcgcga cgtcatctcc cggcggctgc cccttccaga
     4621 agaagaagct cgagctgatc gtctgatcca gcatggcggc gaccgacggc agaagcggcc
     4681 cgcatcggcc cggacctcgc gcgtggcagc gcatgctgtc cggtcggcgg ctcgacctgc
     4741 tcgacccctc gccggccgac atcgagatcg acgacatcgc gcacggactt gcgcgcgtcg
     4801 cgcgctggaa cgggcagacg ctcggcccgc acgcattctc ggtggcgcag cactccctga
     4861 tcgtcgagga gatcgtcggc gcgctgcatc agggcgaggt gtccccatcg gtgaggctgg
     4921 ccgctctgct ccacgatgcg ccggaatacg tgatcggcga cctgatcagc ccgttcaagg
     4981 cggcgatcgg tctcgactac aaggccttcg aggtccgcct gctcggtgcc atccacgagc
     5041 gctatggcct gccacgcgtg ctcgctgaaa cggacgcgcg gatgatcaag gaggccgata
     5101 ggatcgcggc gtacttcgag gcgacgcggc tcgccggctt cgccgacgcc gaggcgcgcg
     5161 agttctttgg gacgccgccg cggctcgatc gagagctgag cttggccttg agcatgcttt
     5221 cggcctcgag tccgacgctg gccggcgcac agtttctcga gcggttcgcc gcgctcacag
     5281 acgccatcac gcgccagcag atctgaagcg catcgcgagc gtgtggtgcc ctgcctggcg
     5341 ccgctttcga atttacaacc ctggccttcg gacctaatcg ttggcgcgag cgccatcgcg
     5401 agccgcagtg tcgtggctgc gatgcctggg caccggtcga caggggatca gacgatgagc
     5461 aaatcgaacg ttgctttgaa cgccgtcagc tatcgctggc cgaagcgccc ggtcgtggtc
     5521 gtgtgcgtcg acggcggcga cccggcctac ttcgatgcgg cgcgcgcgaa ggggatcatt
     5581 ccgaacaccg agcgcttcat gaagcagggc ttctcgggct tcgccgaggc cgtggtgccg
     5641 agcttcacct gtccgaacaa cgtttcgatc attaccggga gtccgcccgc cgtgcatggc
     5701 atctcgggga acttcttcct cgatcaggcg accggcaagg ccgttgtcat gaccgggccc
     5761 gagctgatgc gctcctcgac gatcctcgcc gagttcggcc aaggtgatct cgatcacggc
     5821 aaaggacaag ctccgccagc agctcggcaa gaacatggtc atgtcgcgcg gcgacatcag
     5881 cttttcgtcc gagaaggccg acaaatgcac gatggccgag aacggcatca cgaacgtgct
     5941 cgagctcgtg ggcctgccgc tccccgacat gtactcggcc gagctttcgc tgttcgtcct
     6001 cgaagccggc gtgaagctgc tcgagcgcga gaagcccgac ctcatgtacc tgtcgctcac
     6061 cgactatatc cagcacaagt acgcgcccga cgagggcgag gcgctccgct acatgaagaa
     6121 ttgcgacgag ctcttcggca agctcgaccg gctcggcgcg atcgtggcgc tgacggccga
     6181 ccacggcatg aacgacaaga cgcgcgatga cggcagctat cgcgtggtgt ggctgcagga
     6241 cctgctcgac aaggcattcg gccgaggcaa gacgacggtt atctgcccga tcaccgacta
     6301 tttcgtcgct catcacggcg cactcggcgg cttcgtgcgc gtctacagca acgacaaggc
     6361 cgtgaccgcc aaggccatca tggaggtggc gcgggccgcc aagggcatcg agttcgtctg
     6421 cgacaaggca accgccgtcg agaccttcgg cctccccgcc gaccgcgaag gcgatgtggt
     6481 ggtgatcggc gacgtcggca cctgcatcgg cacgcgcgag caggagcacg acttgaaggg
     6541 cctcgaaggg caccgcctgc gctcgcacgg ttcgacgacc gaggcccgag tgccgctcat
     6601 catcaatacg ccgctcaacg ccgagtatgc ggcgaaagcc aagcgcggcg gcatccgcaa
     6661 tttcaacgtc ttcgagtatg ccgtgaacgg gacggcgtag gccctctcag cggcagccgc
     6721 cgcccgccga gatcgagccg ccgaccttga tggagcagca ggcgttctgc tgctcgtagg
     6781 gccgcccgat cgcctcggag agtgccacgg cgccatcggc cgggaaggcg atgccgtcga
     6841 ggccggccat cacggcgtag gcgtcagtga cgcgcttgtg catgcccgcg ggtcgcgcgc
     6901 agccgagcag cacggggcgt cccggcaagc ggcggcgggc ttcgaggaag atgcggccga
     6961 catccaccgt ggccggcgtg acgaacgtgc ccggccgcgc atagaatggc atgacgacca
     7021 cgagcacgag cgagtgaatt ggctggcgcg cgcaaatgtc gagggccgcg gcctcgccga
     7081 cgatgcgccc gtaatgcagc ccgatgacga tgtgcggcac gatctccatc gtcgttgagc
     7141 aaagggccgc gagcgtttcc tcgaagtcgg cgacacgtcg gtccagatgg tagacctcac
     7201 ggatcgtctc ctcggcgccg atcacgtcca tcatggccgt gtcgacgccg gcactctcca
     7261 tcgccttcgc ccgcggccga tcgaggaggg ccgaatgaat tgcgatccgc aggtgcgggt
     7321 ggtcgcgctt cagccgctcg atgacgggga gatacttctc gtacttgatc tcgttgcgcc
     7381 ggttcgagcc gcccgagagc aggaagcctt gcagatcctg caggagaatg aggtcgcgca
     7441 ccttgcgctc gagcatctcg ggggaggtcg ccgggatcat cggctcgagg atcttcgcct
     7501 ggcagtgatc gcagtcgagg gcgcaggcgc cggccgtcac cgagaacgcg gggaatgagt
     7561 tcttggagca gcctttgagt tcggaggtcg cgtactcctt gaacgttggc gtcgagaagc
     7621 ggatcggcgc gaggccgcct tgctctgcgg ccgcacgctg catctcgcag acgagggccg
     7681 cgtcgaacgc gccgtcctcc agcgcctcga tccgcgcgag ggtatcgagc caggggcgat
     7741 cctcgatcac gctcgcagtc gtcatgtccg gctccgcacg accttcacct cgccgacgtt
     7801 gccggccttc accgtgccct tgcgtttgag gtacgagccg accacgtccc agatcggcgg
     7861 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccagccg gcgacggtgt agtcgcggcc
     7921 cggctcgatc ggcgtaccgg ttttcagatg cgtcatgccg gagatgcgcg agcccaccgg
     7981 cttgtcgacg tcgatcgtgt agcccatgcc gccgacgcgg accatgtcgc cgccctgctg
     8041 gtaatatggg tccttgttga agaggttgtc ggcgacatcc tcgagtatcg ctttcagcat
     8101 ctcgcccttc atggggagac gatagcactt cggatagctg atcgccgtcg cattgtagac
     8161 cgtgtcccag gtgatccctt cgcctggcag cacggaggtg ccccagcgga agcctggcga
     8221 taggcagatc tctgcatcac gctcggcgag catggcatcg cagatgacgt tgtcgagtgt
     8281 gccgttgaag ttgccacgcc ggtagaggag gtcctccgtg cgggcgagct cggtcgccag
     8341 catcgcggca tgtggctcgc gcaccttggc gatgtgtgcc gccatctccg gatcgggccg
     8401 gatggcatca gccagcaccg gaatcagcgc atagcggtaa tcggccatcc tgccgtcgcg
     8461 catctcgata tcgaggcgcg agaggaactt cgtgtgcgat ccggaggcga tgagtagcgt
     8521 cctgccgacc ttgatcggca cagggagtgc atcgtgcgtg tgggcggtca ggatcacgtc
     8581 gatgccggag acgcgcgccg cgagcttgcg gtcgacgtcg aagccgttgt gggaaagcag
     8641 gatgacgagc tcggcacctg cggcccgtgc cgccgtcacc tgcttccgca cgtccttttc
     8701 gcggatgccg aacgaccatt tcggcatcat ccagcgcggg ttggcgatcg gtgtgaacgg
     8761 gaaggcctga ccgatgatgg cgatcttcac cccgcccttc acgacatgct tcacggcctt
     8821 gaaagccggc tcgtcgaact cggtctcgcg gatgttggcg gcgaggaagc ttgccttgac
     8881 ggagcccgcc ttctcgctgc cgccgaacag ctcgccgacc cgcttctcgc cgcgcgtgaa
     8941 ctcccagtgc cccgtggtcg cttccgctcc gagcatgtcg agcacggccg ccatgtcggc
     9001 gccgttgctc ttcatgacgg tgtaggagcc gtgcagcgta tcgccgccgt cgaggaagag
     9061 cgtgcggtcg gcgccgcgct cggcgcggat cgccttcacg agcgtcgcca tgcgatccat
     9121 gccgccgacc cggccatagg tcttggcgag cgccgaaaaa tcatcgtccg agagcatgta
     9181 acactggtgc gagcctgccg ggatgccgaa cttcgcaaga aactcctggc ctgtgagatg
     9241 cggcggctct ccgtacactt cgccgacacc gagattgacc gacggctcgc ggaagtagtg
     9301 cggtagcagc tggccgtggc agtcggtcat gtgaaggagg gtcacctgcc ctttcgcgtc
     9361 gaacttcaga aggtcggcct cgccgatctt ctgctgcgcc gacagtcggg caagtgagcc
     9421 cgagcccgcc gtcagagccg acgccgccag cgctacctga atgaagtccc gtcgcgaaag
     9481 catcgagttc gaaccccgga agttaacgat ctggcggccc gtggtggtgg cgccaaacga
     9541 tcgtcagcat atattcgaat gtgttgaagc gcgagatgcg tccatcacgg cgacccttgt
     9601 ctgtcgcgcg gcaagttgat gcgccttgaa gctcaggtca atcgcgcctg cttcgcttga
     9661 tcgcgaggcc gatctcgtag gcgcgccggc gggcgacgcc gagctcggcg gcaacggcgt
     9721 cagcggcgtc tctgagtgag ctgtcggcga gcttcactct aagcgccgca tcgatcgcct
     9781 cgtcaccgat ctcgacagcc acgggcggag caatcaccag gacgacctcg ccttgtggcg
     9841 gctcgtccgc tgccccggcg gcaagctccg ccaatgtgcc ccgccgcacc tcctcgaagc
     9901 gcttggtgag ttcgcgcgcg atcgcggccg gtcggctacc gagcactgcg gagagatctg
     9961 cgagcgcttc cgccacccgg cccggggcct cgtagatcac gagcgtcgcc ggaacggttg
    10021 agatttcctt gatgcgtttc acgcgctgcc cgtgccgtgg cggcaggaag ccggcgaagg
    10081 tgatcgggcc gggcagcgcc accttgagcc gtcggcccgg cgccatccgc ttcagctgtt
    10141 cgaactcttc c
//
LOCUS       DAORNH010000159        10130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4922_length_10130_cov_2.576179, whole genome shotgun sequence.
ACCESSION   DAORNH010000159 DAORNH010000000
VERSION     DAORNH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4922_length_10130_cov_2.576179"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PK264_15465"
     CDS             <1..569
                     /locus_tag="PK264_15465"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRD77306.1"
                     /translation="IGAAMIGWFGCAMLCYVTPKEHLGLPDRGDVKHGVITYKIAAHA
                     ADLAKGHPAAQLRDDALSRARFEFRWEDQFNLGLDPDTAREYHDATLPKDAHKVAHFC
                     SMCGPKFCSMKITQDVRDYAAKTNAGKSAMVEAEKGMAEMSKKFREMGSQVYLDADKV
                     EVAKTAGHLPGEAGSSAEAVKASNKALG"
     gene            883..1113
                     /locus_tag="PK264_15470"
     CDS             883..1113
                     /locus_tag="PK264_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRD77307.1"
                     /translation="MGDQSLRIAIDAEACGGRPCIRGLRIRVKDILDMLADGAARQDI
                     LVDYPYLEDEDITAALEFASCATDHPVIVAAE"
     gene            1372..2808
                     /locus_tag="PK264_15475"
     CDS             1372..2808
                     /locus_tag="PK264_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017959214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD77308.1"
                     /translation="MPATAAPVSVATLDRLKAVVGPSGYLASADDMSPHEKSWRGNWE
                     GEAALVLRPQSTDEVAALVRICHETGTAIVPQGGNTGLTYASQPGKGRTEVVISTGRM
                     RRIRGLDLANDTIAVDAGVVLKEIQDAAAKADRLFPLSLGAEGTCQIGGNISSNAGGI
                     QVLRYGNTRSLVLGLEVVLPDGRIWDGMRALRKDNTGYDLKQLFIGAEGTLGIITGAV
                     LKLYPKPTDQQSAWIGIGSPEDGVALLGHMRSHLGESVTSFELLARTIIDLLLEGVPG
                     HTDPLAERHPWYVLMEVTGQGGPGTLEGPLAAALEGAMEKGLVRDAVIASSGEQSRKL
                     WKMREDLAFAQQYAGGSIAHDVSVPLSRITEFIRRTDTALTARYPTIRHCCFGHVGDG
                     NLHYNPIRPLDWTRERFAAETAAVNKIVHDIVVELGGSISAEHGIGRLRLEENLRYKS
                     VVETDLMNMVKRALDPGNIMNPGKVVRI"
     gene            2891..3601
                     /gene="trmD"
                     /locus_tag="PK264_15480"
     CDS             2891..3601
                     /gene="trmD"
                     /locus_tag="PK264_15480"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRD77309.1"
                     /translation="MTATRPWRARILTLFPELFPGPLGASLPGQALASGLWSLEAIDV
                     RAFGIGRHRTVDDTPAGGGAGMVMRADVVAAAVDHAARAALELPRLYMSPRGEPLTQA
                     LVRELAFGPGVIVLAGRFEGVDERAIEARGLREVSIGDYVLAGGEVAAMVLIEAAVRL
                     IPGVMGAAQSLTEESFEGGLLEYPQYTRPREFEGRQIPDILLSGDHARIAAWRRAEAE
                     KLTHQRRPDLLVSRQRAR"
     gene            complement(3611..3940)
                     /locus_tag="PK264_15485"
     CDS             complement(3611..3940)
                     /locus_tag="PK264_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77310.1"
                     /translation="MSRSRIVVLITSAVAVILSAPSARSDPGLMALGEHLAQECTSCH
                     RKDGSEKGIPPIVGWKVPEFVTTMRFYKTGERTNPAMVSVAKSLDDDQLTALAMYWGS
                     LKRPGRK"
     gene            complement(4023..5300)
                     /locus_tag="PK264_15490"
     CDS             complement(4023..5300)
                     /locus_tag="PK264_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD77311.1"
                     /translation="MTINRRQFAKLLGAAGAAGTLPTTFAYAQGGAKVVIVGGGPGGA
                     TVAHYVKKAAPALDVTLVEASAQYTTCFFSNLYLGGFRTFESLVHTYDGLRSMGIKVV
                     TAMATDVDAAKREVKLSTGASLAYDRLVLSPGIDFIWESVPGYSEEVAKTMPHAYKAG
                     EQTRILRSRLDAMEDGGVVVMVAPPTPYRCPPGPYERMCMIAHHLKTKKPKSKLVVLD
                     PKREIISKGPVFNEAFNGMYKGIIENNLSNDIDSYVVTRIDPKTMEIETKAGKKVKAA
                     VANIIPAMKAGQIAFKAGATEGNWCPVNPGDFSSKKVANVFVLGDASVAGAMPKSGFS
                     ANSQAKVVANVIVADLAGKPKFPARYRNTCWSMLGPDNSVKVGANYKPEDLSAFEGFV
                     SKPGEDAQLRKQTFEESLGWYSGIVSDTFAKSA"
     gene            5671..5961
                     /locus_tag="PK264_15495"
     CDS             5671..5961
                     /locus_tag="PK264_15495"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HRD77312.1"
                     /translation="MFRRIFTAALAVAAFSTTALLPPANASAPFSEYSCNELWEERNG
                     IFAIRGYCFKTERAIARFGRRCYPPYGQLTAQDRRIVEEIRHWERRKGCPVD"
     gene            6063..>7211
                     /locus_tag="PK264_15500"
     CDS             6063..>7211
                     /locus_tag="PK264_15500"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HRD77313.1"
                     /translation="MRTLVAAATIACLASSSNLIEPACAEQTRAPAPTPSRITVTTFA
                     KGLAHPWSIAFLPDQRLIVTERDGRMRIIARDGRVGDPFKGVPTVAATGQGGLLDVVL
                     APDFATTGTIFFSFSEPRDGGLNGTSVARASLVVDEGGRGELRDLKVVFRQQPATRGG
                     LHFGSRLAFAPDGTLFVTLGERYQRDEAQSLEKHWGKVVRINPDGSVPADNPFVGRTG
                     VLPELWSLGHRNPQSAAMHPGTHELWTVEHGARGGDEVNIPRKGRNYGWPLITYGRDY
                     SGARIGAGTARDGLEQPIYYWDPSIAPSGMAFYTSDAAPHWRGNLFVGALAGQLLVRL
                     VLDGEKVVGEERLLTDLGKRIRDVRAGPDGAVWVATDEPRAEIIRVMPL"
     assembly_gap    7212..7226
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7240..>7451)
                     /locus_tag="PK264_15505"
     CDS             complement(7240..>7451)
                     /locus_tag="PK264_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HRD77314.1"
                     /translation="PPSRLGGPKSKTATASAALSADRKAQFLRRIHTGACKTFFTILG
                     PEANEAHRNHFHVDMAERRSGHFCE"
     assembly_gap    7452..7584
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7585..8004)
                     /locus_tag="PK264_15510"
     CDS             complement(<7585..8004)
                     /locus_tag="PK264_15510"
                     /inference="COORDINATES: protein motif:HMM:NF018597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HRD77315.1"
                     /translation="MRDRGPHPTDRRRAEPQVVLSAPSVMTCDLAVAMSKWVREHVQP
                     AARELLGSPVIRVDIMSAYSCRNAYGRSKSKLSEHGRANALDIRAFMLASGHTVDLLA
                     DWGPTKRDIIAAAAAARAAAEKKAAEDARALAARQPAA"
     gene            8572..9648
                     /locus_tag="PK264_15515"
     CDS             8572..9648
                     /locus_tag="PK264_15515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD77316.1"
                     /translation="MTTGGVAIDKCGAASIITLDRPEVLNAVSRDMIAALAAHFPKAA
                     RDPGTYAVVVQSASAKAFSVGGDIRELAALQASDPAAARAALAAEYGCDWLHECFSKP
                     TISLIDGLMMGSGFGLTQYGTHRVAGEGYQFAMPETAIGFFPDCGACYAFARMPGHIG
                     SYLALTGRRVGRADAYRLGLVTHCISREHFEAIRAGLADADCVDPLLDALHEDPEPGE
                     LGDLADVIARCFSAPRVGEIIARLSEESGRNAAFARETAALLEKRSPAALALTHQYVS
                     LARTIDLKAALTMDYRLACRSIAGHDFREGVRALIVEKDNAPRWSPATLAEVDEATID
                     AMFAPQPSDELALRSREQMQAARV"
     gene            9761..>10130
                     /gene="eno"
                     /locus_tag="PK264_15520"
     CDS             9761..>10130
                     /gene="eno"
                     /locus_tag="PK264_15520"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707838.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRD77317.1"
                     /translation="MSQIVDIIGREILDSRGNPTVEVDVVLEDGSLGRAAVPSGASTG
                     AHEAVELRDGGKRYLGKGVEKAVAAVNSEIFDALRGMDATDQRRIDKTLIRLDGTKNK
                     SRIGANAILGTSLAVAKAAAD"
BASE COUNT         1688 a         3232 c         3315 g         1747 t
ORIGIN      
        1 gcatcggcgc cgccatgatc ggctggttcg gctgcgccat gctctgctac gtgacgccga
       61 aggagcactt ggggcttccc gaccgcggcg acgtcaagca cggtgtcatc acctacaaaa
      121 ttgcggcgca cgcggccgac ctcgccaagg ggcacccggc ggcgcagctt cgtgacgacg
      181 ccctgagccg agcacgcttc gagttccgct gggaggacca gttcaacctc ggcctcgatc
      241 cggatacggc gcgcgagtac cacgacgcca ccttgccgaa ggacgcccac aaggtcgcgc
      301 atttctgctc gatgtgcggt ccgaagttct gctcgatgaa gatcacgcag gacgtgcgcg
      361 actatgcagc caagaccaac gccggcaagt cggcaatggt cgaagccgag aagggcatgg
      421 ccgagatgag caagaagttc cgcgagatgg gaagccaagt ctacctcgac gccgacaagg
      481 tcgaagtcgc caagaccgcc ggacacctcc ccggcgaggc cggatcgtcg gccgaggctg
      541 tgaaggcttc caacaaggcg ctggggtagt ggcgcgggcg aaattggagg gctgatctac
      601 ttggcggtat gcggtgcgag tgcacatttg ttcggaaagt ttggatacgc agctggcggc
      661 acctaccgag cactatcgat cggtgcgccg gcattttttt ccggtggtaa tcttatgatt
      721 ccttcccgaa cttggagtga gactgcccga tccggaggaa ctcgacatgt ttgtcgtctc
      781 gggagggatg gctctcgcgt ttccgctctt ttgtcgtctc gcaatggggt gtcaggcatg
      841 tcagaaattt gaaacgccag ggaagctagg ccaggagcgc atattggcga tcaatcgctt
      901 cgcatcgcga tcgacgctga agcttgtggc gggcgcccct gcattcgcgg cctgcgcatt
      961 cgcgtgaagg atatcctcga catgctggcc gacggcgcgg cgcgccagga catcctagtg
     1021 gactacccct atctggaaga cgaggacatc acagcggcac tcgagtttgc aagttgcgcc
     1081 acggatcatc cggtgatcgt cgcggctgag tgaaatgcag cggttcatcg tggacaccaa
     1141 cctgtcgctg gcggcaacgc cgctaccctg cttgcttgcc cacgaccgag gccgtgccat
     1201 ccaatagagg ccgagtcgga ggacgggtca gggcatctgg tgccaagcca tggagacctg
     1261 gctcgtgcca tgcctgcggt ccaccccgca taactcccgg tcgggctctg cgcttgtgct
     1321 gagaggcgcc gctgctatgg tcgcgcgacg ccattcgacc aggacctccc gatgcccgcg
     1381 accgccgctc ccgtctctgt tgccacgctc gatcgattga aggccgtggt cgggccgtcg
     1441 ggctatctcg cctcggcgga cgacatgagc ccgcacgaga aatcgtggcg cggcaactgg
     1501 gagggtgagg ccgctctcgt gctccgcccg caatcgacgg atgaggtcgc ggccctcgtt
     1561 cgcatctgcc acgagacggg caccgcgatc gtcccgcagg gcggcaacac cgggctcacc
     1621 tacgcgagtc agccggggaa ggggcggaca gaggtggtca tctccacggg gcgcatgcgg
     1681 cgcatccgcg ggctcgatct cgccaacgat acgatcgcgg tcgacgcagg cgtcgtcctg
     1741 aaggagatcc aggacgcggc ggccaaggcc gatcggctct tcccgctgag cctcggcgcg
     1801 gagggcacct gccagatcgg cggcaacatc tcctcgaatg cgggcggcat ccaggtgctg
     1861 cgctacggca atacgcggag cctcgtcctc ggtctcgagg tcgtgctccc cgatggccgc
     1921 atctgggacg gcatgcgtgc gctccggaag gacaacaccg gctacgacct gaagcaactc
     1981 ttcatcggcg cggaaggcac gctcggcatc atcacggggg cggtgctgaa gctctatccg
     2041 aagcccacgg accagcagtc ggcgtggatc ggcatcggct cgccggagga cggcgtcgcc
     2101 ctgctcggcc acatgaggag ccacctcggc gagagcgtca cgtcattcga gcttcttgct
     2161 cgcacgatca tcgatttgtt gctcgagggc gtgccgggcc ataccgatcc gctcgctgag
     2221 cgccatccat ggtatgtgct catggaggtg accggccaag gcgggcccgg cacgctcgaa
     2281 ggtccgctcg cggccgcgct cgaaggcgcc atggagaagg gcctcgtccg tgacgcggtc
     2341 atcgcctctt cgggcgagca gtcccgcaag ctctggaaaa tgcgtgagga ccttgccttc
     2401 gcgcagcagt atgcgggggg cagcatcgcc cacgacgtct ccgttccact gtcgcgcatc
     2461 accgagttca tacggcgcac cgacacggcg ctgaccgcgc gttatccgac gatccgacac
     2521 tgctgcttcg gacatgtcgg cgacggcaac ctgcactaca acccgatccg cccgctcgac
     2581 tggacgcgcg agcggttcgc ggcggagacg gcagcggtca acaagatcgt ccacgatatc
     2641 gtcgtcgagc taggcggatc gatcagcgcg gagcacggca tcggccgact ccgcctcgag
     2701 gagaacctcc gctacaagag cgttgtcgag accgacctga tgaacatggt gaagcgcgcg
     2761 ctcgatccgg ggaacatcat gaaccccggg aaggtcgtcc ggatctgacg agtgacatga
     2821 cggccgacac cgcaatcttg cattgcgccg ccgcctgccg cacagatgcg ccatgactga
     2881 gcaatccact atgacggcca cccgcccctg gcgcgcccgc atcctgacgc tctttcccga
     2941 gctctttccg gggccgctcg gcgcgtcgct ccctgggcag gcactggcgt cggggctctg
     3001 gtcgctcgag gcgatagatg tccgtgcctt cggcatcggg cgccatcgca ccgtcgacga
     3061 cacaccggca ggcggcggag ccggcatggt gatgcgcgcc gacgtggtgg ccgccgcggt
     3121 ggaccatgcc gcgcgcgctg ccctggagct gccgcggctc tacatgtcgc cacgtggcga
     3181 gcctctgact caggccctcg ttcgagagct cgcattcggt ccgggcgtga tcgtcctcgc
     3241 cggccgcttc gaaggcgtcg acgagcgggc cattgaggcg cgcgggctcc gcgaggtttc
     3301 gatcggcgac tatgtgctcg ccggtggaga ggtggcggca atggtgctga tcgaggccgc
     3361 cgtgcgcctc attcccggcg tcatgggcgc ggcgcaatcg ctcacggagg agagcttcga
     3421 gggcgggctc ctcgagtatc cgcagtacac gcggccgcgc gaattcgagg gaagacagat
     3481 cccagacatc ctgctttccg gcgatcatgc gaggatcgca gcctggcggc gcgcagaggc
     3541 ggaaaaactg acgcaccagc ggcgtcccga tctcttggtg tcccggcaaa gagcgcgctg
     3601 agaccctcag ctatttccgg cccggccgct tcaatgagcc ccagtacatg gcgagcgcgg
     3661 tcagctggtc gtcgtcgagc gactttgcga cgctcaccat cgcgggattg gtgcgctcgc
     3721 ctgtcttgta gaaccgcatg gtggtgacga attcgggcac cttccaaccg acaatcggcg
     3781 ggatgccctt ctccgagccg tccttcctat ggcacgacgt acattcctgc gcgaggtgct
     3841 caccgagcgc cattaagccc ggatcggagc gggccgacgg cgcactcagg atgacggcca
     3901 cggccgaggt gatcagcacg acaatgcgac tgcgagacat gggtaagcct cgacgtgggt
     3961 cagcgggctg cagcgaatgc gtggcacgcg gcacgctccg atggcgcgcc gcgcgcgttc
     4021 ccttaggccg actttgcgaa tgtatcggac acgatacccg agtaccagcc gaggctctcc
     4081 tcgaaggtct gcttgcgaag ctgcgcgtcc tcgcccggct tcgagacgaa gccctcgaaa
     4141 gccgagaggt cctcgggctt gtagttggcg ccgaccttaa cgctgttgtc ggggccgagc
     4201 atcgaccagc aagtgttgcg gtagcgggcg gggaacttcg gcttgccagc gaggtcggcg
     4261 acgatcacgt tggcgactac cttcgcctgg ctgttggccg agaagcccga cttcggcatg
     4321 gcgccggcta ccgaggcgtc acccagaacg aacacgttcg caaccttctt cgacgagaaa
     4381 tctccgggat tgaccgggca ccaattgcct tcggtcgcgc ctgccttgaa ggcgatctgg
     4441 ccggccttca tcgccggaat gatgttggcg acggcggcct tcaccttctt cccggccttg
     4501 gtctcgatct ccatggtctt gggatcgatg cgggtgacga cgtagctgtc gatgtcgttc
     4561 gagagattgt tctcgatgat gcccttgtac atgccattga aggcctcgtt gaagacagga
     4621 cccttggaga tgatctcgcg cttcggatcg aggacgacca gcttggactt cggcttcttg
     4681 gtcttcaagt ggtgcgcaat catgcacatg cgctcgtagg gtcccggcgg gcagcggtag
     4741 ggcgtcggcg gcgcgaccat gacaacgacg ccgccatcct ccatggcgtc gagccggctc
     4801 ctcaggatgc gcgtctgctc gccggccttg taggcgtgcg gcatggtctt ggcgacttcc
     4861 tcggagtagc ctggcacact ctcccagatg aaatcgatgc cgggcgagag tacgaggcga
     4921 tcgtaggcga gcgaagcgcc agtcgagagc ttgacctctc gtttcgcagc gtcaacgtcg
     4981 gttgccattg ccgtgacgac ctttatgccc atcgatcgga ggccatcata ggtatgcaca
     5041 agcgactcga acgtgcggaa accgccgaga tagaggttcg agaagaagca cgtcgtatac
     5101 tgcgcgctgg cttcgaccag cgtcacgtcg agcgcgggcg cagccttctt gacgtagtgc
     5161 gcgacggtcg cgccgcccgg tccgccgccg acgatcacga ccttggcccc gccctgtgcg
     5221 taggcgaatg tcgttggaag cgtgcccgcg gcaccagcgg ctcccagcag cttggcaaat
     5281 tgacgccgat tgatggtcat cctcagtcct cccagtggcc tcgagctcaa ctcggctttc
     5341 tcatggcagc gttctgtgcc gcgaccttgg cagcagtttg atgcaacgca aaggcattgt
     5401 ctatattcaa atggtcgaat gtttaggtgc gaagctttgg cgtagggtct tcgatcgcga
     5461 gtccaggcgg tcggcggccg tggcgcgatg cgtcgatcgc aacgcatcgg gccggcgtgg
     5521 gaacgcgtcg caaggacttc ttgaccacct atgtcgaggg atacgccgat gctgtggaaa
     5581 tggccgacaa tccggcgcat tgaacctatt gtcagcctac agcgtccaat cggtggacct
     5641 gatccgacca cgcccacagg gagacctcga gtgttccggc gaattttcac tgctgctctt
     5701 gccgtcgcgg cattttcgac gacggccctg ctgcccccgg ccaacgcgag cgctccgttc
     5761 tcggagtaca gctgcaacga gctttgggag gagcgcaacg gcatcttcgc gatccgcggc
     5821 tattgcttca agaccgagcg cgccatcgcc cggttcggtc gtcggtgcta tcctccctat
     5881 ggtcagctca ccgcgcagga tcgacgcatt gtcgaggaga tccgccactg ggagcggcgc
     5941 aaggggtgcc cggtcgactg agacacggga cgagcaggca atcccttcgt cctgatttct
     6001 ccgcaaggcc gtcgtaacag cgaacgtcgg tccttgcggt gctcaggaga acggtcatgg
     6061 tgattcgaac gcttgtcgca gcagccacga ttgcgtgcct ggcgtcctcg tccaatctga
     6121 ttgagcctgc gtgcgcagag cagacgcgtg ctccggcgcc gacgccatcc cgaatcaccg
     6181 tcacgacctt cgcgaaaggt ctcgcgcacc cctggtcgat cgcgtttcta ccggaccagc
     6241 gactcatcgt gacggagcgc gatggccgaa tgcggatcat cgcgcgggat ggtcgcgtcg
     6301 gcgacccgtt caaaggtgtt ccgacggtcg ccgcgacggg tcagggtgga ctgctcgatg
     6361 tcgtacttgc acccgatttc gcgacgaccg gcacgatctt tttctcgttc tcggagccgc
     6421 gtgacggcgg cctcaacgga acgagcgtcg cgcgcgcgtc actggtagtc gacgagggcg
     6481 gccgcggcga gctgcgagac ctcaaggtcg tctttcgcca acagccggcg acacgcggcg
     6541 gcctccattt cggctcgcgc ctcgccttcg cgccggacgg cacgctgttc gtaacgcttg
     6601 gcgagcgcta ccagcgcgac gaggcacagt cgctggagaa gcattggggc aaggtggtcc
     6661 gcatcaaccc ggacggcagc gtgccggcag acaatccctt tgtcggccgc acgggcgtgc
     6721 tgcccgagct ctggtcgcta ggccatcgca atccgcagtc ggcggcgatg catcccggta
     6781 cacacgagct gtggacggtc gagcacggcg cacgcggggg cgatgaggtg aacatccccc
     6841 gcaagggtcg caactacggc tggccgctca tcacctatgg ccgcgactat tcgggagcac
     6901 gcatcggcgc cggcaccgcg cgcgatggac tcgagcagcc gatctactat tgggacccgt
     6961 cgatcgcgcc ttcgggcatg gccttctaca cgtccgacgc agcgccgcac tggcggggca
     7021 atctgttcgt cggcgcgctc gcgggccagt tgctcgtgcg gctggtcctg gatggcgaga
     7081 aggtcgtcgg cgaggagcgg ctgctcacgg atctcggaaa gcgcatccgc gacgtgcgtg
     7141 ctggccccga tggtgccgtc tgggtcgcga ccgacgagcc gcgtgcggag atcatccgcg
     7201 tgatgccgct annnnnnnnn nnnnnncgcc gccacgtcgc tactcgcaga aatggcccga
     7261 gcgccgttcg gccatgtcga cgtggaaatg gttgcgatgc gcctcgttcg cctcggggcc
     7321 gaggatcgtg aaaaacgtct tgcaggcacc ggtatggatg cgacgcagga actgagcctt
     7381 cctgtcagcc gacagagccg cggatgccgt cgcggtcttc gatttcggtc ccccgagccg
     7441 gctcggcggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnncgctgc cggctgcctg gcagcgaggg cgcgggcgtc
     7621 ctccgcggcc ttcttctcgg ctgcggcgcg tgccgccgcg gcagcggcaa tgatgtcacg
     7681 tttcgtcggg ccccagtcgg cgagcagatc aacggtgtgg ccggaggcga gcatgaaggc
     7741 gcgaatatcg agcgcgttgg cgcgcccgtg ctcgctgagc ttcgatttcg agcggccgta
     7801 ggcgttgcgg cacgaatagg cgctcatgat gtcgacgcgg atgacggggc taccgaggag
     7861 ctcgcgagcc gccggctgca catgctctcg cacccacttg gacatggcaa cggccaggtc
     7921 gcaggtcatg accgacggcg cgctgagcac gacctgcggt tctgcccgac ggcgatcagt
     7981 tggatggggg ccgcggtccc gcacgtgccg ccctccttca tgggctctgc tgggatcgcg
     8041 acgacatcgg cccccttgag gagcgcggcg caacgggcga ggccggcgag cacatcaagc
     8101 tccggccatc cgcttttgtg catcttggga gcggccggcg cgggcgctgg cggcgccgct
     8161 gcaggaccag gagttgaggc ggctggagcg ggctgggctg ccgctggtgg ttgcggtgcg
     8221 gcggtctggg cgcccgctgc acctgttgtc agcagcatgg cagcgacaga caggaccggg
     8281 ccgaagacct tcatgcaagt acctcacaag gatggcggcc tgctcgaacc gctcggcagc
     8341 gctcacaggg cgctcgcctc actgcattgt cgtgcatcga cgtcagctcg tatcactcaa
     8401 cccttgatga gtttcatctc gccgaatcgc ttccgaattg caacgataag cacggcgatc
     8461 cgtacaggga aacggcatag tcgcaactgc gaccactggt ggcatgtggt gatcggggac
     8521 atcttgtgca aagagcttgg cggcgatgtg gcagggcagg ggcggcagga catgacgacg
     8581 ggcggagttg cgatcgacaa gtgtggtgcg gcatcgatca tcacgcttga tcggccggag
     8641 gtgctcaacg ccgtctcgcg tgacatgatt gctgcacttg ccgcgcattt cccgaaggcg
     8701 gcgcgcgatc ccggcactta cgccgttgta gtccagtcgg cgtctgcgaa ggccttctcc
     8761 gtcgggggcg acattcgtga gcttgcagcc ttgcaggcaa gcgatccggc ggctgcccga
     8821 gcggcccttg ctgccgagta cggctgtgac tggctgcacg agtgcttctc caagccgacg
     8881 atctccctca tcgacggttt gatgatgggc tccggcttcg ggctcaccca gtacgggacg
     8941 caccgcgtcg ctggcgaggg gtatcaattc gccatgcccg agacggcgat cggcttcttt
     9001 cccgattgtg gcgcctgcta cgccttcgcg cgcatgccgg ggcacatcgg cagctatctc
     9061 gccctgacag gccggcgcgt cggccgcgct gatgcctatc gcctcggcct cgtcacgcat
     9121 tgcataagcc gcgaacactt cgaggcgatc cgggccgggc tcgcggacgc cgactgcgta
     9181 gacccgctgc tcgatgcgct ccatgaggat ccagagcccg gcgagctcgg cgacctcgcc
     9241 gatgtgatcg cgcgctgctt ctcggcgccg cgcgtcggcg agatcatcgc gcggctatcg
     9301 gaggagagcg ggcggaatgc agcatttgcc cgggagacgg cagcgctcct cgaaaagcgc
     9361 tcgcccgcgg cgttagccct cacgcatcag tacgtgagcc ttgcccggac aatcgatcta
     9421 aaagccgcgc tgacgatgga ctataggctg gcctgccgca gcatcgccgg gcatgatttc
     9481 agggaaggcg tccgggcact catcgtcgag aaggacaacg ctccacgctg gagccccgcg
     9541 acgctcgccg aggtcgacga ggcgacgatc gacgccatgt tcgcaccgca gccgagtgac
     9601 gagctcgccc tgcggagccg agaacagatg caggcagcgc gcgtctgagc cgcgtccagg
     9661 gcgcccaagc tgcgcccgag ttggcacttt caaggcccgc ccgcctcggc tacaaggcgc
     9721 gcgtcgccac gcgtttgcca gccatcggag agggtgcccc atgtcccaga tcgtcgacat
     9781 cattggccgc gagatcctcg attcacgcgg caacccgacg gtcgaggtcg acgtggtgct
     9841 cgaggacggc tcgctcggcc gcgccgccgt gccgtccggc gcctcgactg gcgcgcacga
     9901 agccgtcgag ctgcgcgatg gcggcaagcg ctacctcggc aagggcgtcg agaaggccgt
     9961 cgccgccgtc aattccgaga tcttcgacgc gctgcgcggc atggacgcga ccgaccagcg
    10021 ccgcatcgac aagacgctca ttcgcctcga cggcaccaag aacaagagcc gcatcggcgc
    10081 caacgcgatc ctcggcacct cgctcgctgt cgccaaggcc gcagccgacc
//
LOCUS       DAORNH010000160        10122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4928_length_10122_cov_2.338433, whole genome shotgun sequence.
ACCESSION   DAORNH010000160 DAORNH010000000
VERSION     DAORNH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_10122_cov_2.338433"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PK264_15525"
     CDS             <1..670
                     /locus_tag="PK264_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HRD77318.1"
                     /translation="SLPSRDAAHMIRLMGDRLDAIEAGFGIDVMTLAATRTSPLAPGQ
                     SAAAGGAGNSADDDRLARLVDSLANRLESGRILRLALGSSHIPERASLAMAAMAEAGP
                     ERASLDGTELHRRAPPRPPTLLPDPEPIEVLSEVPEGVPQRFTWRRLTRRIAAARGPE
                     RIEPEWWRNLGIVAHEAAPLWRTRDYWQIEDEWGARYWVFRDGRWCSPGARQPRWYLH
                     GLYG"
     gene            728..>1706
                     /locus_tag="PK264_15530"
     CDS             728..>1706
                     /locus_tag="PK264_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRD77319.1"
                     /translation="MPSLPYAELQAISNYSFLEGGSHPDELVARAKELGLAALAIADR
                     NTLAGVVRAHVAAREAGLRLIVGARLELTPTGKGDPPVLPALICLPTDRAAYGRLCRL
                     LSLGQMRAEKSRCRIHLDDVAAHAEGQILIAIPPPGWSWRETRLSGSGLPQGAPAEGS
                     PGSAGRIIRFPGRNKQGPEDPLARLLTRMRRTLAPAQLYLAASHRLGGDDELRLSALA
                     RIATAARTPLVATGDILYHSPERRPLQDILACIRLKTTIDRAGLLLAANAERHLKRPD
                     EMAKLFAAHPQAVARSLEIAEACRFSLDELVYEYPDEPVPAGTTAQAHLE"
     assembly_gap    1707..1770
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1771..4132
                     /locus_tag="PK264_15535"
     CDS             <1771..4132
                     /locus_tag="PK264_15535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006642559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HRD77320.1"
                     /translation="ALISELGFAHYFLTVHDIVRFARGRGILCQGRGSAANSAVCYCL
                     GVTAVNPAEIDLLFERFISEARREPPDIDVDFEHERREEVIQYIYERYGRHRAGLAAT
                     VISYRSRSAVREVGKAMGLSEDTVAALAGQVWGTRSGDALPAAYVRGAGLDPDDPRLA
                     ATLALTAELTGFPRHLSQHVGGFILTRGPLVELVPVGNAAMADRTFIEWDKDDIAALG
                     LLKVDILALGMLTCIRKALVMIERHHGRRETLASIPREDRAVYDMLCRADSIGVFQVE
                     SRAQMNMLPRLKPRTFYDLVIEVAIVRPGPIQGDMVHPYLRRRSGLEPEDYPAPAPEH
                     GNKDELRQILGKTKGVPLFQEQAMRIAMVAARFSDSDVNELRKSMATFRRRGTIGLLE
                     EKMIGAMVARGYDPAFASRCFNQIKGFGEYGFPESHAASFAHLVYVSAWIKCHYPAAF
                     AAALLNSQPMGFYAPAQIVRDAREHDVEARPVDVNLSDWDATLEPAPCPGGVALRLGL
                     RQIDGFKEADAQAILAARGAGGAYRDIEEVGERSGLGPSPLERLAAADAVRSLGQDRR
                     QALWDVKGIPSGSPLPLFAWSEARAAGREPVVALPAMALSEHVVNDYQTQRLSLKAHP
                     MHFLRERMSARRVQACRTLQQMKSGAFARVAGVILVRQRPGSAKGVVFMTIEDETGIA
                     NTVVWPRTLERFRSVVMGARLVLVEGRVQRHECIIHVVASRIENLSSWLDHLSQMAEE
                     IAIPIANADEVRRPEPGSARGTLQTSPRLARHPRAERIIPKSRDFH"
     gene            complement(4126..5484)
                     /locus_tag="PK264_15540"
     CDS             complement(4126..5484)
                     /locus_tag="PK264_15540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRD77321.1"
                     /translation="MGASGSFEHDRSAVGHGAVLRIALPITLSNATGPLTGVALIAVI
                     GQSQGAAEIGGVAVAVAIFSMLLWTFSFLRMATTGLAAQALGAGDRREIAGHGLRALL
                     VAACGGVLLIMLQWPIAAAALELMRVSPVVAASAREFFDLRVWSMPAALVNFAVFGWL
                     IGLGQARRAFEIQLVLNVLNVALAAALVLGLGFGVRGLAVATVIAECLGMIAGLWVAR
                     REAARLGAVAPLADAVDLARLRRSMAMNSDIAIRALAHHFVLAFFTSEGAAQGDATLA
                     ANAILYNLLSVMIFLLDGFAFAAETFVGQAIGAQDRQAFEQAARITTRWAVLFAVIVG
                     VVAWFAGDALIALAVKSEEVRAEARTFLVWIALSPLAGVWAFQLDGIFVGATRSRDMR
                     NTMLASVAAFFVFWWPLSASFGNHGLWAAYTLFFVVRAILLGLRLPGLVEASFAGRVR
                     HQ"
     gene            5641..8304
                     /locus_tag="PK264_15545"
     CDS             5641..8304
                     /locus_tag="PK264_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HRD77322.1"
                     /translation="MSQRRLPRQTSTGVAAERHHVSEQQTEGDPDDAARLLPDVVRNW
                     FQRKGWTPRQHQLALLSAAAERQSTLLIAPTGAGKTLAGFLPSLVGAHRRMVAGEASP
                     DAISTLYISPLKALAVDIARNLAQPISEMGLGVRIETRTGDTPASRRARQRSKPPHIL
                     LTTPEQISLLLSGEDAPYIFRGLDTVILDELHALAPSKRGDLLALGLSRLRRLAPGLL
                     IFGLSATVARPSELRAFIVPQACPGDRTDLARLVELKGGAPPRISILDSDGPLPWAGH
                     TTLYAIDAIYRAIQSARLALLFVNTRMQAELLFHALWRVNEQSLPIALHHGSLDVAQR
                     RKVEAAMADGRLRAVVATSTLDLGIDWGDVDLVIHVGAPKGASRLLQRVGRSNHRLDE
                     PSRALLVPSNRFEALECRAAIAAASEGSQDAVLNRHGALDVLAQHIMGRACSGPFDPG
                     ELFAEVTSTLPFATLSRASFDRAIDLVATGGYALRAYERFARIRRDDDGQLRVSHPRF
                     AQQYRLNIGTIVESPLVKVRLVGRRTRPVRHVAGAGGRILGEVDEWYLGELTPGDTFM
                     LGGVVVRFETMREGEALVTRANASEPMIPSYSGGKFPLSTHLAGRVRRMLADRTTWTD
                     LPASVVEWLALQAERSTLPAENDVLVETFPRNGRHYLVLYPFEGRLAHQTLGMLLTRR
                     LNRHGRKPLGFTANDYALSIWCASDLSSWIRSDPSTLTALLAEDMLGDDLDDWLAESG
                     LMKRTFRMCAIIAGLIERRHPGKDKSGRQLTVSATLIYDVLRRYDPQHMLLEAARTDA
                     ATGLLDIHRLGAFLSRIRGGICHIDLSRASPLSVPLLLEIGRESVHQALTDDLLREAA
                     ALVHDATGRNGDWAADRQIGSTAAAAAGLPK"
     gene            8399..9121
                     /gene="pdeM"
                     /locus_tag="PK264_15550"
     CDS             8399..9121
                     /gene="pdeM"
                     /locus_tag="PK264_15550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HRD77323.1"
                     /translation="MHELRPDRGSITDYGSQLIEIGGKILHGDMTGALWWPSERALIV
                     ADLHLEKASFLAMRGQPLPPYDTRETLQRVALVLDRYEPELVVALGDSLHDRGALDRI
                     GEDERRILTILQEARRWVWITGNHDGGTAAALGGEVRDQMTIAGLKLRHEPRPGPVTH
                     EIAGHLHPAARLSMYGHAIRRPCFVGNGLRLVLPAFGTFTGGLNVLDEAFTGLFRNDG
                     MSVWMLGQEGVYPVATRHLKED"
     gene            complement(9171..9320)
                     /locus_tag="PK264_15555"
                     /pseudo
     CDS             complement(9171..9320)
                     /locus_tag="PK264_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(9448..10071)
                     /gene="istB"
                     /locus_tag="PK264_15560"
                     /pseudo
     CDS             complement(9448..10071)
                     /gene="istB"
                     /locus_tag="PK264_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
BASE COUNT         1776 a         3474 c         3160 g         1648 t
ORIGIN      
        1 gagcctgccg agccgggacg ccgcacacat gatccgcctg atgggcgacc ggctcgatgc
       61 gatcgaggcc ggcttcggca tcgacgtgat gacgctcgcg gcaacgcgca cttcccctct
      121 cgcgccaggc cagtcggccg ctgcaggggg cgccggcaac agtgcggacg acgaccgcct
      181 cgcgcgcctc gtcgacagtc tcgccaatcg gctcgagagc gggcgcatcc tgcgtctggc
      241 gctcggctcg agccacatcc ccgagcgggc gtcgctcgcc atggctgcca tggcggaggc
      301 cggcccggag cgtgcctctc tcgatgggac agagctccat cgccgcgccc ctccccgccc
      361 gccgacgctg ctgcctgatc ccgagccgat cgaggtgctg tccgaggtcc ccgagggtgt
      421 gccccagcgg ttcacctggc gccgcctcac gcgtcgcatc gccgccgcca ggggacctga
      481 gcggatcgag cccgagtggt ggcgcaacct cggcatcgtg gcgcacgagg cagccccgct
      541 ctggcgcacc cgcgactact ggcagatcga ggacgagtgg ggcgctcgct actgggtgtt
      601 ccgcgacggc cgctggtgct cgcccggagc ccgacagccc cgctggtacc tccatggcct
      661 ctatggatag caggtccacg cccattgccg cttcgatcca gattggcgag atgccgcgga
      721 gaaattgatg ccgagcctcc cctatgcaga gctgcaggcc atcagcaact actccttcct
      781 ggagggtggc tcacatccgg acgagctcgt cgcacgcgcg aaggagctcg gccttgcagc
      841 gctcgccatc gccgatcgca atacgctcgc aggcgtcgtg cgtgctcacg tcgcagccag
      901 agaggcaggt ctccggctca tcgtcggcgc acgcctcgag ctgacgccga cgggaaaagg
      961 cgatcccccc gttcttcccg cgctcatctg cctgccgacc gaccgggccg cctacggccg
     1021 gctctgccgc ctcctctcac tcgggcagat gcgcgccgaa aagagccggt gccggatcca
     1081 tctcgatgac gtcgccgcgc acgccgaagg gcaaatcctg atcgccatcc cgccacccgg
     1141 ctggagctgg cgcgagacgc ggctctccgg ctccggatta ccgcaaggag cacccgccga
     1201 gggttcgccc gggagcgccg gccggatcat ccgttttccc ggtcggaaca agcaaggccc
     1261 ggaggatcca ctcgccaggc tcctcacgcg catgcgccgc acgctcgcac cggcgcagct
     1321 ttatcttgct gcgtcccatc gcctcggcgg cgacgacgag ctccggcttt ctgcgcttgc
     1381 ccgcatcgcg accgccgccc gcacgccgct cgtcgcgacc ggcgacattc tctaccactc
     1441 gccggaacgc cgcccgctcc aggacatcct cgcctgcatc cgcctgaaaa cgacgatcga
     1501 tcgcgccggg ctcctcctcg ccgccaatgc cgagcgccat ctgaagcgcc cggacgagat
     1561 ggccaagctc ttcgccgccc atccgcaggc cgtggcgcgc agcctcgaga tcgccgaggc
     1621 ctgccgcttc tcgctcgacg agctcgtcta cgaatatccg gacgagcccg ttcccgcggg
     1681 cacgaccgcc caggctcacc tcgagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgcgctcatc agcgagctcg gtttcgccca
     1801 ttacttcctg accgtgcacg acatcgtgcg cttcgcacgg ggccgcggca tcctctgcca
     1861 gggccggggg tccgccgcaa attcggccgt ctgctattgc ctcggcgtca ccgccgtcaa
     1921 cccggcagag atcgatcttc tcttcgagcg cttcatctcg gaggctcgcc gcgagccgcc
     1981 cgatatcgac gtcgatttcg agcacgagcg gcgggaggag gtgatccagt acatctacga
     2041 gcgctacggc cgccaccgcg ccgggctcgc ggcgaccgtc atctcctacc gctcgcgctc
     2101 ggcggtgcgc gaggtcggca aggccatggg cctctcggag gacactgttg ccgcgctcgc
     2161 ggggcaggtg tggggaacgc gctcgggcga tgcactgccg gccgcctacg tgcgcggcgc
     2221 cggcctcgat cccgacgacc cgagacttgc ggcaaccctc gcgctcaccg ccgagctcac
     2281 cggcttcccg cgtcacctct cccagcacgt cggcggcttc atcctgacac gcgggcctct
     2341 cgtcgagctc gtccccgtcg gcaacgccgc catggccgat cgcaccttca tcgagtggga
     2401 caaggatgac atcgccgcct tgggcctcct caaggtcgac atcctcgcgc tcggcatgct
     2461 gacctgcatc aggaaggcgc tcgtcatgat cgagcgccac cacggccggc gggagacgct
     2521 cgcctcgatc ccgcgcgagg atcgagccgt ctacgacatg ctctgccggg ccgacagcat
     2581 cggggtgttc caggtcgaga gccgcgccca gatgaacatg ctgccgcgcc tgaagccgcg
     2641 caccttctac gacctcgtca tcgaggtggc gatcgtccgg ccggggccca ttcagggcga
     2701 catggtgcac ccctacctgc ggcggcgctc cggcctcgag cccgaggact atcccgcccc
     2761 cgcccctgag catggcaaca aggacgagct tcgccagatc ctgggcaaga ccaagggcgt
     2821 cccgctcttc caggagcagg cgatgcgcat cgccatggtg gctgcgcgct tctcggacag
     2881 cgatgtaaac gagctccgga agtccatggc gaccttccgg cgccgcggta ccatcggcct
     2941 cctcgaggag aagatgatag gggccatggt cgcacgcggc tacgacccgg ccttcgccag
     3001 ccgctgcttc aatcagatca agggcttcgg cgagtatggc ttccccgaaa gccacgcggc
     3061 gagctttgcc cacctcgtct atgtgtccgc ctggatcaag tgccactatc ccgcagcttt
     3121 cgcggcagcc ctcctcaact cgcaacccat gggcttctat gcgccggctc agatcgtccg
     3181 cgatgcccgc gagcatgacg tcgaggcgcg cccggtcgat gtcaatctct cggactggga
     3241 tgcgacgctc gagcccgccc cctgtccagg aggtgttgca ctgcggctcg gtctccgcca
     3301 gatcgacggc ttcaaggaag cggatgccca agccatcctc gccgcccgcg gagcaggtgg
     3361 agcgtatcgc gacatcgagg aggtcggcga gcgatcgggc cttggtccct ctccgctcga
     3421 gaggcttgct gccgccgatg ccgtccgctc tctcggtcag gaccgccggc aggcgctctg
     3481 ggatgtgaag ggcattccct cgggaagccc attgccgctg tttgcctgga gcgaggcgcg
     3541 ggccgcgggc cgcgagccgg ttgtggcgct tcccgcgatg gcgctctcag agcacgtcgt
     3601 caacgactat cagacgcagc gcctgtcgct gaaggcgcac cccatgcact tcctgcgcga
     3661 gcgcatgagt gcccgtcgcg tccaggcctg ccgcacgcta caacaaatga agagcggagc
     3721 tttcgcgcgc gttgccggcg tcatccttgt ccgccagcga ccgggatcgg ccaaaggcgt
     3781 ggtcttcatg acgatcgagg acgagaccgg catcgccaac acggtcgtgt ggccgcggac
     3841 gctcgagcgc ttccgctccg tggtcatggg cgcgcgcctc gttctcgtcg agggacgcgt
     3901 tcagcgccac gaatgcatca ttcacgtcgt cgccagtcgt atcgagaacc tcagctcatg
     3961 gctcgatcat ctctcgcaga tggccgagga gatcgccatt cccatcgcca atgccgacga
     4021 ggtgcgccgc cccgagcctg gcagcgcgcg cggcacgctg cagacctctc cgcgcctcgc
     4081 acgtcatccg cgcgcggagc gcatcattcc aaaatcgagg gattttcact gatgccgcac
     4141 cctgccagca aacgacgcct cgacgagccc cggcaaacgc aggccaagca agatcgcccg
     4201 aaccacaaag aacagggtgt aggccgccca gaggccgtga ttgccgaaag aagcgctcag
     4261 cggccaccag aacacgaaaa aggccgccac actggcgagc atcgtattgc gcatatcgcg
     4321 ggaccgggtc gccccgacga aaattccgtc gagctgaaaa gcccacacgc cggcgagtgg
     4381 cgaaagcgct atccagacaa ggaacgtcct cgcctccgcc cggacttcct cgctcttcac
     4441 ggcaagcgcg atcaacgcgt caccggcaaa ccatgccacg accccgacaa tgacggcgaa
     4501 gagcacggcc caccgcgtcg tgatgcgtgc cgcttgctcg aacgcctggc ggtcctgcgc
     4561 tccgatcgcc tggccaacga aggtttctgc cgcgaatgca aatccatcga gcagaaagat
     4621 catcacagag aggagattgt agaggatcgc gttcgccgca agcgtggcgt cgccctgtgc
     4681 ggcgccctca gaggtgaaga acgccaggac gaaatggtgc gccagggcac ggatcgcgat
     4741 atccgagttc atggccatcg agcgtcggag cctggcgaga tcgacggcat cggcaagagg
     4801 cgcgacggca ccgagcctgg cggcctcgcg tcgagccacc cacagccccg cgatcatccc
     4861 gaggcactct gcgatcacag tcgcgacagc gaggcctcta accccaaacc cgagcccaag
     4921 gacgagtgcg gcggcaagcg caacattcaa gacattgaga acgagctgga tctcgaaggc
     4981 acgtcgggcc tgaccaaggc cgatcaacca gccgaaaacc gcgaaattga cgagcgcggc
     5041 cggcatcgac cagacgcgaa ggtcgaagaa ctcgcgagcc gatgcggcaa cgaccggcga
     5101 gacccgcatc agctcaaggg ccgccgcggc gatcggccac tgcagcatga taaggagcac
     5161 gccgccgcat gccgccacca gcagcgcgcg caggccgtgg cccgcgatct ctcgcctgtc
     5221 gcccgctccg agcgcttgcg ccgcgagacc cgtcgtcgcc atcctgagga agctgaaggt
     5281 ccacagcaac atgctgaaga tcgccaccgc aactgcgaca cctccgatct cggccgcacc
     5341 ctgcgattgg ccgatcactg cgatcagcgc aactccggtc aggggacctg tggcattgga
     5401 gagcgtgatg ggaagcgcga ttcgcagcac cgccccatgc ccgactgcgg accggtcatg
     5461 ctcgaacgaa ccgcttgccc ccacgatgcc ccatcctatt ctcagatact tcggccgcca
     5521 acgcgatcgc cgatgcactc gagcgtagcg gcaaagcccg aaatgcgcag cgacgatgat
     5581 taccggtgtc gtgcagccgt gctatgcaga aggccttcat ctgccatgga aaagcttggc
     5641 atgtcgcaga gacgactgcc gcgacagacg tccaccggcg ttgcagccga acggcaccac
     5701 gtgtctgaac agcagaccga aggcgacccc gacgatgccg ctcggctgct acccgatgtc
     5761 gtgcgaaatt ggtttcagcg aaagggctgg accccccgcc agcatcagtt ggcattgctg
     5821 tcggctgctg cggaacggca atcgaccctg ctaatcgccc cgaccggagc cggcaagacg
     5881 cttgccggat ttctcccgag cctcgtcggc gcgcaccggc ggatggtagc cggcgaggca
     5941 agcccggatg cgatatcaac gctttacatc tcaccactca aggcgctcgc tgtcgacatc
     6001 gcccgcaatc tcgcgcaacc gatttccgag atgggcctcg gcgttcggat cgagactcgc
     6061 acgggcgata cgcctgcctc gcggcgtgcc cgccagcgca gcaagccccc gcacatcctg
     6121 ctgaccacgc cggagcagat ctcgctgttg ttgtcgggtg aggacgcgcc atacatcttc
     6181 cgtgggctgg acacggtcat tctcgacgag ttgcatgcac tcgctccctc gaagcgcggc
     6241 gatcttctgg cactcggcct cagccggttg aggcggctcg cgccgggcct cttgatcttc
     6301 gggctttcgg caacggtcgc aagaccgtcc gaactcaggg ccttcattgt accccaggcg
     6361 tgcccgggcg acagaacgga cctcgcacgc ctcgtcgagc tcaaaggtgg tgcacctcca
     6421 cgaatctcca tcctcgacag tgacgggccc ctcccttggg cgggccacac gacgctctat
     6481 gccatcgatg cgatctatcg agctatccag agcgcgcggc tcgcgctgct cttcgtcaac
     6541 acgcgcatgc aggcggagct cctctttcat gccctctggc gcgtcaacga acagagcctg
     6601 cccattgcgc tgcatcatgg ctcgctcgac gtcgcgcagc gacgcaaggt cgaggccgcc
     6661 atggcagacg gacggttgcg tgctgtcgtc gcgacctcga cgctcgatct cggcatcgac
     6721 tggggcgacg tcgacctcgt catccacgtt ggtgcgccca aaggcgcaag ccgcctactc
     6781 cagcgcgtcg gtcgctccaa ccatcggctg gacgagccct cgcgcgctct tctcgtcccc
     6841 tcgaaccgct tcgaagcgct cgaatgcaga gctgccatcg ccgccgcgag cgaaggaagc
     6901 caggacgcag tcctcaatcg gcatggagca ctcgacgtgc ttgcgcagca catcatgggg
     6961 cgagcctgct ccggcccctt cgacccgggc gagctcttcg cggaggtgac gagcacgctg
     7021 ccgttcgcca ccctctcgcg agcatcgttc gatagggcca tcgacctcgt tgcgaccggc
     7081 ggatatgcac tgcgagccta cgagcgcttt gcacgcatcc gccgagatga cgacggccaa
     7141 ctccgagtgt cacatccccg cttcgcccag cagtaccgtc tcaacatagg cacgatcgtc
     7201 gaaagcccgc tcgtcaaggt ccggctcgtc ggccgccgca ctcgccctgt ccggcacgtc
     7261 gccggagcag gcggtcggat cctcggggaa gtcgacgaat ggtacctcgg tgagctcaca
     7321 cccggcgata ccttcatgct tggtggggtc gtcgttcgct tcgagaccat gcgcgaagga
     7381 gaggcattgg tcacgcgcgc aaacgcgagt gagccgatga ttccgagcta ttcaggtggg
     7441 aaattcccgc tttccacgca ccttgcgggc cgggtgcgcc gcatgctcgc ggatcggacc
     7501 acttggacag atctcccggc ctcggtcgtc gagtggctcg ctttgcaggc agagcgctca
     7561 acactcccgg ctgagaacga cgtgctcgtt gagaccttcc cgagaaacgg ccggcactac
     7621 ttggtactct atccattcga agggcgtctc gcacaccaga cactcggtat gctgctcacg
     7681 cgtcgtctca atcgacacgg acggaagccg ctcggcttta cagccaacga ttatgcgctg
     7741 tcgatctggt gcgcatcgga cctgtcgagc tggatccgat ccgatccgag caccctgacg
     7801 gcgctgctcg ccgaggacat gctcggagac gatctcgacg attggctcgc cgaaagcggg
     7861 ctgatgaagc ggaccttccg gatgtgcgcc atcatcgccg ggctcatcga gcgacgccat
     7921 cccggcaaag ataagtccgg ccggcaactg acggtctcgg ccaccctcat atacgacgtg
     7981 ctgcgtcgat acgacccgca gcatatgctt ctcgaggcgg cgcgcaccga tgcggcaacg
     8041 ggcctgctgg atattcacag gctcggcgca tttctatcgc gaatacgggg tgggatttgc
     8101 catatcgatc tttcgcgagc gtcgcccctg tcggtgccgc tacttctaga gatcggacgc
     8161 gaatccgtac atcaagcact gaccgacgat ctcctgcgag aggctgccgc actggtgcac
     8221 gatgccaccg gccggaatgg agattgggcg gccgacaggc aaatcgggtc cactgcagct
     8281 gccgcagcag gcctgccgaa gtgagatcgc agaggctcat gtcccgcggc ttcggccgcg
     8341 gacgccgacc tgaaggcagt gaggcgtaac gacaagaagc aacgatggga cattgcaaat
     8401 gcacgagctt agacccgatc gcggatcgat caccgactac ggctctcagt tgatcgagat
     8461 cgggggaaaa atccttcatg gcgacatgac cggcgcgctc tggtggccgt ccgaacgggc
     8521 cctgatcgtt gccgatctcc acctcgaaaa ggcctccttc ctagccatgc gtgggcagcc
     8581 cttgccaccg tacgacacac gcgaaacgct gcagcgtgtt gctcttgtgc tggatcgcta
     8641 cgagccggaa ctcgtcgttg cgctcgggga cagcctccat gaccggggcg cacttgatcg
     8701 gatcggcgag gacgagcggc gcattctgac aatcctacaa gaagcccgtc gctgggtttg
     8761 gattaccgga aatcacgatg ggggaacggc agcggctctt ggaggcgaag tccgagacca
     8821 aatgactatc gcaggactga agttgcgcca cgagccgcgg ccaggccccg tcactcacga
     8881 gatcgcggga catctgcatc ccgcggcccg tttatcgatg tacggccatg cgattcgacg
     8941 accgtgcttc gtcgggaacg gcctgaggct ggttctgccg gcattcggca ctttcacggg
     9001 cggattgaac gtcctcgacg aggcatttac cggtctgttc aggaatgatg gaatgtccgt
     9061 gtggatgctc ggtcaggaag gcgtatatcc cgtcgccaca cggcacctga aggaggactg
     9121 agccaaggca cgcggtaagc atccggagag tgccgaagcg gcggctgccg tcaggccgcg
     9181 cgggtgacgg cagctttctg agagagccag ttccacggca gcaactcatc cagccgatgg
     9241 gcgggctgct cggcgataca ggcgaggacg tcggcgagcc atgcctgcgg ggcgatgtcg
     9301 ttcagctttg cggtgtcaat cggcgtcgaa ctttgacccc ggatcgaact gcaatgttga
     9361 ccccgtccat ccgtggtcgt tgcgctggcc aagcctggag ctgcggagcg gaagcggagc
     9421 agtggagggc ttggccggcg ggcgcagtca ggagcggtgc ttgaggcgcc agctgtcgtt
     9481 gccggtctcg acgatgtcgc agtgatgcgt gaggcggtcg agcagcgccg tcgtcatctt
     9541 ggcatcgccg aacacggctg gccactcggc gaaggcgagg ttggtcgtga cgatgatgga
     9601 cgtgcgctcg tagaggcggc tgatcagatg gaacagcagc tggccgccgg actgggcgaa
     9661 cggcaggtag ccgagttcgt cgagcaccag caggtcgacg cgagcgatac cgtcggtgat
     9721 gcgtccgccg cgcccgagct tcatctcggc ttcgagtttg ttgacgaggt cgacggcgtt
     9781 gaagaagcgc gccttgcgcc cggcccggat gacgctgcgc gcgatgccga tggcgatgtg
     9841 gctcttgccc gtgccggtgc cgccgatcag cacgacgttc ctctgctcgt cgaggaaggt
     9901 gccggtcgag agttgctcga caagtgcgcc gttgatcgga gtgccagcga agtcgagatc
     9961 ggccagctcc ttcgccagcg gcagcttggc gatgcccatc tggtagttga tcgagcgcgc
    10021 ctgcttggcc gcgatctccg ccttgagcag tgccccgatc accttcttgg gcggcgacaa
    10081 tcaaggtgag aactcagcga caatcaggat gagagagcgc ca
//
LOCUS       DAORNH010000161        10067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4962_length_10067_cov_2.535757, whole genome shotgun sequence.
ACCESSION   DAORNH010000161 DAORNH010000000
VERSION     DAORNH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4962_length_10067_cov_2.535757"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PK264_15565"
     CDS             <1..594
                     /locus_tag="PK264_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77324.1"
                     /translation="KSLDAERQAMRGERAEDAYAKAIETIAVADSRDDIATLMREARR
                     TPTTEDDVIVRRISDIDHAIERAEREVERQQQALADHVRRRQEVERVRDRFRGAGYDH
                     PSATFNNGEAIASVLAEVMKGVVRSGVLWDVLRGGYQSRRPRGNRDFGSPGFPFPFPM
                     PDGRDTRGGDWREPDTSGGWSPSDGGGSDDFRTGGEI"
     gene            893..1651
                     /locus_tag="PK264_15570"
     CDS             893..1651
                     /locus_tag="PK264_15570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77325.1"
                     /translation="MAGHGKVALITGGGTGIGRSAALHLQSDGWNVAVTGRRKEELDK
                     TCALAKAGGGKMVAIAADCGKQPDVKRLFAEVLKSFGRLDFLFNNAGMGLPAGPMEDI
                     PVDKWQQVVDINLTGSFLCAQEAIRIFKAQKPQGGRIVNNGSISAHVPRPMSVAYTAT
                     KHAVTGLTKCIALDGRPYNIACGQLDVGNADTAMGGRMKAGVPQANLEIRPEPVMDVE
                     HCGQAIRFMASLPLESNIFTMTIKATNMPFEGRG"
     gene            1946..2938
                     /locus_tag="PK264_15575"
     CDS             1946..2938
                     /locus_tag="PK264_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012485187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRD77326.1"
                     /translation="MFNELGGFSSAVIAMLALLVIVLYNTIRIVPQGCNWTVENFGRY
                     TRTLSPGLHILVPVVERIGRKMNVMEQVMDIPSQDVITKDNAMVRVDGVAFYQVLNSA
                     KASYEVSNLEGSIVNLTMTNIRTVMGSMDLDELLSNRDQINSRLLQVVDAATETWGVK
                     VTRIEIKDIAPPRDLVESMARQMKAERDKRASILESEGMRQAAILKAEGEKQAVMLAA
                     EGRKEAAFRDAEARERAAGAEAKATELVSSAIAGGNVQAINYFVATAYVKALESLAKS
                     PNQKILMMPLDASSVIGSLAGLAEITKDAFSGTGSPPPKGGRDRGDGGVPRHQA"
     gene            complement(2997..>3261)
                     /gene="glnQ"
                     /locus_tag="PK264_15580"
     CDS             complement(2997..>3261)
                     /gene="glnQ"
                     /locus_tag="PK264_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191694.1"
                     /note="similar to ATP-binding component of ABC
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HRD77327.1"
                     /translation="RIMLFDEPTSALDPEMIKEVLDVMIELARSGMTMLCVTHEMGFA
                     RSVADRVIFMDRGEIVEEGPPLEFFNNPRSERTKLFLSQILNH"
     assembly_gap    3262..3297
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3298..3725)
                     /locus_tag="PK264_15585"
     CDS             complement(<3298..3725)
                     /locus_tag="PK264_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77328.1"
                     /translation="MRNVNKWYGEFHVLKSINLNVAQGERIVICGPSGSGKSTLIRCI
                     NRLEEHQQGDIVVDGTELTSDVRNIERIRAEVGMVFQSFNLFPHLTVMDNLCLAPVWV
                     KKMPRAKAEEIAMTYLKRVKIPEQAMKYPGQLSGGQQQRVA"
     gene            4033..5118
                     /locus_tag="PK264_15590"
     CDS             4033..5118
                     /locus_tag="PK264_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HRD77329.1"
                     /translation="MITIKAKSLEELVRDIFAKAGCSLAEGERIARYLVTANLTGHDS
                     HGVQRVPRYLQWKEEGVFVADRTVKVVTDTPVLAVVDGQYGFGQTIAPQAVQIGIDKC
                     KAMGLSAVALRNVGHIGRVGDWAEMAAAEGLVSVHFVNAASSVLVAPYGGVERRFSTA
                     PYCVGVPRKGAHPLVLDFATSIVAEGKVLNASFGGKAIPDDALIGPDGTMSGDPHALY
                     GDYTPSGPRSYRQGKGAIRAFGEHKGSGLAFMCEILGGSLTGTGAASESRKWSNGMLS
                     FYIDPARVDPTGFFPEDMGKYAAYVKSSKPVEPGGEVLLPGEPEARMRAERLASGVPL
                     PDDTWAAILAAARKVGVDERRIQQATM"
     gene            5844..6209
                     /gene="dksA"
                     /locus_tag="PK264_15595"
     CDS             5844..6209
                     /gene="dksA"
                     /locus_tag="PK264_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HRD77330.1"
                     /translation="MNEKQRLYFKRKLLTWKEEIIRQTRETIEGLHADSVQHADLADR
                     ATSETDRALELRARDRQRKLISKIDEALARIDTGSYGFCEETGEPISLKRLDARPIAT
                     LSIEAQERHERRERVFRED"
     gene            complement(6279..>8126)
                     /locus_tag="PK264_15600"
     CDS             complement(6279..>8126)
                     /locus_tag="PK264_15600"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRD77331.1"
                     /translation="SPVLAGDVAEAIAAAAAKAGPGAKSLRFLSIANLHNDCLPGAEL
                     AAARMAVTKLLNSLTWKSTPIAVTPIGPSGTLLQVSLEDLGWVPAHWDMILYAYPYGF
                     MPESRASRSAAELTGTGLPVVRADWLAYAAGRAPLYYDLLGLPTQTPELWKVAKIDPA
                     EAIEKRLVKRGGVVASSVMTSERLLERFAASGGAHWLSYEVPVGAKPRLLDLPTPGTP
                     PAGFRHELNLVLLSLPNGLPATFVAEASGTRLDAVPPGALRHKGLQPSAAMPAGTACL
                     ACHDTGPRRFRDDVRANVLGDTRFAPEFQDLVRALHPQIEELDQLLSIDIEATMDALK
                     RAGVDPAIRLHGHEIINGLARLYLRPVNLARLAGELGVSTERLIERSEGRDAEIRLLI
                     LRLQQGAVPRREVEMHLGTLGELADLARPAPFAGQAAQPLPSDGRGADADIGLVLVSD
                     KTVYRPGDLATFTVRTKRDCFLTVINLDTLGRATVIFPNEFEQSNRITAGRELRLPSE
                     RAPYQLRAKQKGRERMIAICNTAGPWAEGITHDYERQRFTVLGDYSVYLSRGAGADTL
                     EKSPPAAKPPDRRAPVPTKRRPAVAQATEGDPRPPAEALARTGIVIEIK"
     assembly_gap    8127..8159
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8160..8592)
                     /locus_tag="PK264_15605"
     CDS             complement(<8160..8592)
                     /locus_tag="PK264_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77332.1"
                     /translation="MIRVPMHRRLTALLGAVLALATIIAQSLESPAQDTPALPRPVAD
                     APIAAPSDHAAAKAFAVLEKHCASCHQTGRLSRPSPSGGLGNILRLDELARLGAVVQP
                     GNPDGSKLVDYMMRREMPFDIFHEGVNREPPTGEEIDHVRAW"
     gene            complement(8681..9403)
                     /locus_tag="PK264_15610"
     CDS             complement(8681..9403)
                     /locus_tag="PK264_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRD77333.1"
                     /translation="METSAKPGGASDDRSTVRTWDVPTRLFHWTLVTLIVMAWVSRKY
                     GDTNLVWHTWNGYAILVLIVYRIIWGFVGGSTARFSSFVSGPVTAVRYGLDFATRKPR
                     HFLGHNPLGGTMVLALLAVVGFQATIGLFSYEGADAFVGGPLSSKVSSEVTTLLTTWH
                     IRIFDLIIIMAGLHITANIVYLFWKPENLIKPMLTGRKPKADYEDQQEAEFGSIGKAL
                     VCLVVATILVFGTIKVLGGRIF"
     gene            9527..>10067
                     /locus_tag="PK264_15615"
     CDS             9527..>10067
                     /locus_tag="PK264_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD77334.1"
                     /translation="MRPIARVLAAVLVALLVAAPAHAQGKRVALVIGNSAYAVGPLAN
                     PGNDADAIATALKALDFDVTLQKNLGREPMRAALGAFGRNVPGSDMAVVFYAGHGTEI
                     AGRNYLIPTDARLARAGDVDLETIALDTVLAQLDGAKLLKLVILDACRNNIFPLSGAK
                     RTLSRGLARVEPEDNTLVVY"
BASE COUNT         1771 a         3158 c         3436 g         1633 t
ORIGIN      
        1 aaaagcctcg atgccgagcg ccaggcgatg cgcggcgagc gcgccgagga cgcctacgcg
       61 aaggcgatcg agaccatcgc tgtcgccgac agccgcgacg atatcgcaac attgatgcgc
      121 gaggcgcgcc gcacgccgac tacggaggac gacgtcatcg tgcgacgcat ctcggacatc
      181 gatcacgcga tcgaacgggc cgagcgcgag gtggagcgcc agcagcaggc gctcgccgat
      241 cacgtccgcc gccgccagga ggtcgagcgt gtgcgcgacc gcttccgtgg tgccggctac
      301 gaccacccct ccgcgacctt caacaatggc gaggcgatcg cgagcgtgct cgccgaggtg
      361 atgaagggcg tcgtgcgctc gggtgtcctc tgggacgtgc tgcgcggcgg ctaccagtcg
      421 cgccgcccgc gcggaaaccg cgacttcggc tcgccgggct tcccgtttcc gttcccgatg
      481 ccagatggcc gagacactcg aggtggcgac tggcgcgagc ccgacacgtc cggtggctgg
      541 tcgccctcgg acggcggcgg gagcgatgac ttcaggacgg gcggcgagat ttgacagcgg
      601 gccgaagccg gcgctctgga tcgatagagg agcccattct cgcggccatc gcgcgaggct
      661 tcaaggcact gaccgtcggc gcggttgccc tcagcgacgt caaccgctgc gctcatggcc
      721 tggccgctct ctgccgtggc cgctctcggg atccgggccc tcgtctttcc gtgcgggcag
      781 tgcccctctc gtcaagtcgg ccactgaccg ctccgaagag aattgtgctc gctgcctgca
      841 ccgtctggtg tagcctcggg gttcagcatc cagaagcacg atggaggttc gaatggctgg
      901 gcacggcaag gtggctctga tcacgggagg gggcacggga atcggccgtt cggcggcact
      961 gcatctgcag tccgacggct ggaatgtcgc tgtcacggga cggcgcaagg aggagctcga
     1021 caagacatgc gcgctcgcca aggcgggggg cggcaagatg gtggcgatcg cggccgattg
     1081 cggcaagcag ccggacgtga agcggctgtt cgccgaggtt ctcaagagtt tcgggcggct
     1141 cgatttcctg ttcaacaatg cgggaatggg actgcccgcg gggccgatgg aggatattcc
     1201 ggtcgataaa tggcagcagg tcgtcgatat caatctgacg ggctcgtttc tctgcgcgca
     1261 ggaagcgatt cgcattttca aggcgcagaa gccgcagggc ggtcgcatcg tcaataacgg
     1321 ctcgatttcg gcacatgtgc cgcggccgat gtcggtcgcc tacacggcga cgaaacacgc
     1381 cgtcaccggg ctcacgaagt gcatcgccct cgacggtcgg ccctacaaca tcgcctgcgg
     1441 ccagctcgat gtcggcaacg ccgatacggc gatgggtggg cgcatgaagg cgggcgtgcc
     1501 ccaagcgaac ctcgagatcc ggcccgagcc ggtgatggac gtcgagcatt gcggccaggc
     1561 gatccgcttc atggcgagcc tgccgctcga aagcaacatt tttaccatga cgatcaaggc
     1621 gacgaacatg cccttcgagg ggcgcggctg acgatcgtgc acgacgatgg tgggccgctg
     1681 tctcagcaat gaggcgcggc ccggctaaac cattccacga caatacgagc gcagggacgg
     1741 gcgaagagac gggcgaagaa tagtaacagc caagatacga atcttgccaa tttatgcatt
     1801 gcccccatat tcgcaggcaa aggcttggcg ttcgtcgaaa tttacgcgcc aggtcgacac
     1861 ggtcttttac gccccgtttg ggtttcatgc ccaggatcgc cgcaggaata atccgcaacg
     1921 catccgtcac caaggagtaa atgcgatgtt caatgaactc ggcggtttct cgtcggccgt
     1981 catagcgatg ctcgctctgc tggtcatcgt cctctacaac acgatccgta tcgttcctca
     2041 gggctgcaac tggacagtcg agaacttcgg ccgctacacg cggacgctgt cacccgggct
     2101 ccacatcctc gtaccggtcg tcgagcgcat cgggcgcaag atgaacgtca tggagcaggt
     2161 gatggacatc ccctcgcagg acgtcatcac caaagacaac gccatggtcc gcgtggacgg
     2221 agtcgccttc tatcaggtgc tcaattccgc caaggcctcc tacgaggtct ccaatctcga
     2281 aggctcgatt gtcaatctca ccatgaccaa catccgcacg gtcatgggct cgatggacct
     2341 cgacgagctg ctctcgaacc gcgatcagat caactcgcgc ctcctccagg tcgtggacgc
     2401 tgccaccgag acgtggggcg tgaaggtcac ccgcatcgag atcaaggaca tcgcgccgcc
     2461 gcgcgacctc gtcgagagca tggcccgcca gatgaaggcc gagcgcgaca agcgcgcctc
     2521 gatcctcgaa tccgagggca tgcgtcaggc ggccatcctg aaggcggagg gcgagaagca
     2581 ggccgtcatg cttgccgccg agggccgcaa ggaagccgcg ttccgcgacg ccgaagcacg
     2641 cgagcgcgca gccggtgccg aagccaaggc gacggaactc gtcagcagcg ccatagccgg
     2701 cggcaatgtg caggcgatca actacttcgt ggcgaccgcc tacgtgaagg cgctcgagag
     2761 cctcgcgaag tcgccgaacc agaagatcct gatgatgccg ctcgacgctt cctccgtgat
     2821 tggttcgctc gccggtctcg ccgagattac caaggacgcc ttctcaggga cgggcagccc
     2881 gccaccgaag ggcggccgcg accgcggcga cggcggcgtg ccgcgtcatc aggcctgaag
     2941 gcggacgcgc taccaccggg aatcgagtgg ggcgcccatc ggcgccccgt ttccgtttag
     3001 tgattgagga tttggctcag gaacagtttg gtgcgctccg agcgcggatt gttgaagaac
     3061 tcgagcggcg gtccttcctc gacgatctca ccacggtcca tgaagatcac gcgatcggcg
     3121 accgagcggg caaagcccat ctcgtgcgtg acacagagca tcgtcatgcc ggagcgcgcg
     3181 agctcgatca tgacgtccag gacctccttg atcatctcgg ggtcgagggc ggaggtcggc
     3241 tcgtcgaaca gcatgatgcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcg
     3301 acacgctgct gctggccgcc cgagagctgg cccggatact tcatggcctg ctcgggtatc
     3361 ttgacgcgct tcaggtaggt catggcgatc tcctcggctt tcgcacgagg catcttcttc
     3421 acccagaccg gggcgaggca gaggttgtcc atcacggtca ggtgcgggaa gagattgaac
     3481 gactggaaca ccatgccgac ttcggcccgg atgcgctcga tgttgcgcac gtccgaggtg
     3541 agctcggtac cgtcgacgac gatgtcgccc tgctggtgct cctcgaggcg gttgatgcag
     3601 cggatgagcg tcgacttccc cgagcccgac gggccgcaga tgacgatccg ctcgccctgc
     3661 gccacattca gattgatgct cttcagaacg tggaactcgc cgtaccactt gttcacgttg
     3721 cgcatcagga ttgcggcctc ggccgatcct gctgcggcgg gcgtactgat tgcggcggcg
     3781 gcgttcgaca cgtgcattct ccctcgacgg aatgggcacg cacaagggcg tcccggggaa
     3841 gatcctgcga ttgaggccga gtatggccga tcggccggcc cagggcaacg tttgccggca
     3901 gtgtcccgtc ggggatggtt gtcggaattg tcagacgagg catctcgcga ctgccattga
     3961 gcggcagggc ttgagcgggc aaggtgccag tatcacgatc cggtcaaccg aacctgaagg
     4021 acacgacgcc agatgatcac catcaaggcc aagtcgctcg aggaactcgt tcgcgacatc
     4081 ttcgccaagg ccggttgctc gctcgccgaa ggcgagcgga tcgcgcgcta cctcgtcacc
     4141 gcgaacctca ccgggcatga cagccacggg gtgcagcgcg tgccccgcta cctccagtgg
     4201 aaggaggagg gcgtgtttgt cgccgaccgg accgtgaaag tcgtgacgga tacgccggtg
     4261 cttgcggtcg tcgacggaca gtacgggttc ggccagacca tcgcgcctca ggccgtccag
     4321 atcggcatcg acaagtgcaa ggcgatgggg ctctcggcgg tggcattgcg caatgtgggc
     4381 cacatcggcc gcgtcggcga ctgggcggag atggcggcgg cggagggcct cgtctcggtg
     4441 cacttcgtca acgcggcttc gagcgtgctg gtggcgccct acggcggcgt cgagcggcgc
     4501 ttctcgacgg cgccctattg cgtcggcgtg ccgaggaaag gcgcccatcc cctcgtcctt
     4561 gacttcgcga cgtcgatcgt tgccgaaggc aaggtgctca atgcttcgtt cggcggaaag
     4621 gcgatcccgg acgacgcgct catcgggccg gatggaacga tgagcggcga tccgcacgcg
     4681 ctctatggcg actacacgcc atcgggtccc agaagctatc ggcaggggaa gggcgcgatc
     4741 cgcgccttcg gcgagcacaa ggggtcgggg ctcgccttca tgtgcgagat cctcggcggc
     4801 tcgctcaccg gcacgggggc ggcgtcggag agccgcaagt ggtcgaacgg aatgctctcg
     4861 ttctacatcg atccggcccg cgtcgatccg acgggatttt tcccggagga catggggaag
     4921 tatgcggcct atgtgaagag ttcgaagccg gtcgagccag gaggcgaggt gctgcttccg
     4981 ggcgagcccg aggcacgcat gcgtgccgag cggctcgcca gcggcgtccc gcttccggac
     5041 gacacctggg ccgccatact cgcggcggcg cgcaaggtcg gcgtcgacga gcggcgcatc
     5101 cagcaggcga cgatgtgagg cggcgtcggt ccgcccgaaa cgatgcgagc gccactccgg
     5161 ggcggagtgg cgctcgatac ccctgccgct gaccgggcgg cgggagcggg ccgcggcgag
     5221 acgcgtgggg aatgctcgcc ggcggattgg gagagactgc gagggctcct gtcgttcttg
     5281 ttcttcgcac tcccagcggc atgcgatggc gcgacgtgag ggggttgccg tgaacctgcc
     5341 tgctcgtggg aagcaggagg ttctcgccac cgtatccagt tgagccgaag agaggggcga
     5401 gctcaaccgg agcgtttcgt gccgtcgaaa cacaaccata ggtagcgcaa cgaggttaat
     5461 acaccctgaa gaggtcgaac tcctctccag tggagagcca aatttttcca gttttttctt
     5521 ttgcgcgacg ggccgcggac ttcactgccc aaatcagccc gcaaggtcgt cacagaagcg
     5581 tcgcgcagtc atgctcctct tgacttggaa atacagtcac ttattaggtg gcacgcgctg
     5641 tgcttcgacg ttcgtcgtgc aatggtttgg acccgccgat ccgcctgcag gagccgcccg
     5701 gaatggtgaa gctggacatc aagactaagg ggaagacgaa ggccgcgccc agcggcggag
     5761 caagtgccag caaggcacgc aaccccaagg atagctcgat cgtgctgcct gacgactatc
     5821 gaccgtcgga aaaagagccg ttcatgaacg agaagcagcg gctctacttc aagcgcaagc
     5881 tgctcacctg gaaggaagag atcatccgcc agacgcgcga gacgatcgaa gggctgcacg
     5941 cggattcagt gcagcacgcc gatctcgcgg atcgcgccac gtccgagacg gatcgcgcgc
     6001 tcgagctcag ggcgcgggat cgccagcgca agctcatctc gaagatcgac gaggcgctcg
     6061 cccgcatcga taccggcagc tacggcttct gcgaggagac gggcgagccg atcagcctga
     6121 aacggctcga cgcgcgtccg atcgcgacct tgtcgatcga ggcgcaagag cggcacgagc
     6181 ggcgcgagcg cgtgttccgg gaagactgag tcttgatcat gcgtggaccg gtcgcgagac
     6241 gtcgggactg agccgcgtgt gcgtcgatcg ctgcggctct acttgatctc tatcacgatg
     6301 ccagttcgcg cgagcgcctc cgccggcggg cgcggatccc cttccgtcgc ctgcgcgaca
     6361 gccggcctgc gcttcgtggg aaccggcgcc cggcgatccg ggggcttggc tgccggcggc
     6421 gacttctcga gcgtatctgc gcccgcgccg cgcgaaagat agaccgagta atcgccgagc
     6481 acggtgaagc gctggcgctc ataatcgtgc gtgatgccct cggcccaagg cccggccgta
     6541 ttgcagatgg cgatcatccg ttcgcggcct ttctgcttgg cgcgaagctg gtagggagcc
     6601 cgctcgctcg gcaggcgcag ctcccgcccg gcggtgatcc ggttcgactg ttcgaactcg
     6661 ttcgggaaga tcacggtcgc acggccgagc gtatcgaggt tgatgacggt caggaagcag
     6721 tcgcgcttcg tgcgcacggt gaaggtcgca agatcgcccg gacgatagac cgtcttgtcc
     6781 gagacgagca cgaggccgat gtcggcgtcc gcaccacggc cgtcgctggg gagcggctgg
     6841 gcggcctggc cggcaaacgg cgctgggcgc gcaaggtcgg cgagctcacc gagtgtgccg
     6901 agatgcatct cgacctcgcg ccgcgggacg gccccctgct gaagccgcag aataagcaaa
     6961 cggatctcgg catcgcgacc ttcgctccgc tcgatcaggc gctctgtcga gacgccgagc
     7021 tcgccggcga ggcgggcgag gttgacgggg cgcagataga ggcgggcaag cccgttgata
     7081 atctcgtggc cgtgcaagcg gatcgcggga tcgacaccag cacgcttcag tgcgtccatc
     7141 gtcgcctcga tgtcgatact gagcagctgg tcgagctcct ctatctgcgg atgcagcgcg
     7201 cggacgaggt cctggaactc gggcgcgaat cgcgtgtcgc cgagcacatt tgctcgcaca
     7261 tcgtctcgga agcggcgcgg cccggtatcg tggcaggcaa ggcaggctgt tccggccggc
     7321 atggcggcgc tcggctgcag gcccttgtgg cggagcgcgc ccggaggcac cgcgtcgagc
     7381 cgcgttccgg atgcttcggc gacgaaagtg gccggcagcc cgttcggcag cgagagaagt
     7441 acgagattca attcgtggcg gaacccggcg ggcggcgtgc cgggtgtcgg caagtcgagg
     7501 agacgcggct tggcgccgac ggggacctcg tagctcagcc aatgagcgcc acccgaggcg
     7561 gcgaagcgct cgagcagccg ctccgacgtc atcacactcg aggcgacgac gccgccgcgc
     7621 ttcacaagtc gcttctcgat cgcctcggcg ggatcgatct tggcgacctt ccagagctcg
     7681 ggcgtctggg tcggcaggcc gagcaggtcg tagtagaggg gcgcgcggcc ggcggcatag
     7741 gcgagccaat cggcgcgcac gacagggagg cccgtgcccg tcagctccgc ggcgctgcgc
     7801 gaggcgcggc tttcgggcat gaagccatag gggtaggcat agaggatcat gtcccagtgg
     7861 gccggtaccc agccgagatc ttcgagcgag acctgaagaa gggtgcctga ggggccgatc
     7921 ggcgtcacgg cgattggcgt gcttttccag gtaaggctgt tcaggagctt ggtcaccgcc
     7981 atgcgcgccg cggcgagctc ggcccccggc aggcagtcat tgtggaggtt ggcgatcgaa
     8041 aggaagcgca gtgatttcgc tcctgggcca gccttcgcgg ccgccgcggc aatggcctcg
     8101 gcaacgtcgc cggcgagcac gggactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     8161 ccaggcccgg acgtggtcga tctcctcgcc agtcggcggc tcgcgattga ccccctcgtg
     8221 gaagatgtcg aacggcatct cccggcgcat catgtagtcg acgagcttcg agccgtcggg
     8281 gttgccgggc tggacgactg cgcccaggcg cgcaagctcg tcgagcctga ggatgttgcc
     8341 gaggccgccc gacgggctcg ggcgactgag gcgccccgtc tgatggcaac tcgcgcagtg
     8401 cttctcgaga acggcgaagg ccttggcggc ggcatggtca ctcggggcag cgatcggcgc
     8461 atcagcgacc gggcgtggca gggctggcgt gtcctgcgcg gggctttcga ggctctgggc
     8521 gataatcgtg gcgagtgcca gtacggcccc gaggagcgcg gtcagcctgc ggtgcatcgg
     8581 tacgcggatc acggcgggct ggtcccttgc actgtctcaa acacacatcg cgggcgccgg
     8641 gcgaggccgg tcctcgacgg accggcgccg ccccgcgcga tcagaagatc cgaccgccga
     8701 gcaccttgat cgtgccgaat acgaggatgg tagccacgac gaggcagacg agtgccttgc
     8761 cgatcgagcc gaactcggcc tcctgctggt cctcgtagtc cgccttgggc ttcctcccgg
     8821 tcagcatcgg cttgatcagg ttctccggct tccagaacag atagacgatg ttggccgtga
     8881 tgtgcaggcc cgccatgatg atgatgagat cgaagatgcg aatgtgccac gtcgtgagca
     8941 gcgtcgtcac ctcggacgag accttcgagg aaagcggccc gccgacgaag gcgtccgccc
     9001 cctcgtagga aaagaggcca atagtcgcct ggaagccgac gaccgcgagg agggccagca
     9061 ccatggtgcc gccaagcggg ttgtggccga ggaaatggcg gggcttgcgc gtcgcgaagt
     9121 cgaggccgta gcgcacggcc gtcacgggtc cggaaacgaa gctcgaaaag cgggccgtgg
     9181 agccgccgac gaagccccag atgatccgat agacgatgag gacgaggatc gcatagccgt
     9241 tccaggtgtg ccagacgagg ttggtatcgc cgtacttgcg gctgacccag gccatgacga
     9301 tcagggtgac gagcgtccag tggaagagcc gcgtcggcac gtcccaggtc cgcacggtgg
     9361 agcgatcgtc ggacgcgccg cccggcttcg cactggtctc catggtggtc tccttgactg
     9421 ccgctggcgg aaattcgcat ggcgcgcgtt cggcttcaac cgaggcggcg ttgacggcag
     9481 gggagttcgt gcgccaacat cgcgccgttg agagagggac attgcgatgc gtccaatcgc
     9541 ccgagtactt gctgccgtgc tggtcgcgct gctcgttgcc gcgcccgccc acgcgcaggg
     9601 aaagcgcgtc gccctggtga tcggcaattc ggcctacgcg gttgggcccc tcgccaatcc
     9661 ggggaacgat gccgacgcca tcgccacggc gttaaaagca ctcgatttcg acgtgacctt
     9721 gcagaagaac ctcggccgcg agccgatgcg agcggcactc ggcgccttcg ggcgcaacgt
     9781 gccggggagc gacatggcgg tcgtgttcta cgccggccac ggcaccgaga tcgcggggcg
     9841 caactacttg atcccgaccg acgcgcgcct cgcgcgtgcc ggcgacgtcg acctcgagac
     9901 gatcgcgctc gacacggtgc tcgcccagct cgatggcgcc aagctgctga agctcgtgat
     9961 cctggatgcc tgccgcaaca acatcttccc gctctcgggc gccaaacgca cgctctcgcg
    10021 cggcctcgcc cgcgtcgagc cggaggacaa caccctcgtc gtctacg
//
LOCUS       DAORNH010000162        10050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_4973_length_10050_cov_2.560080, whole genome shotgun sequence.
ACCESSION   DAORNH010000162 DAORNH010000000
VERSION     DAORNH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4973_length_10050_cov_2.560080"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1140)
                     /locus_tag="PK264_15620"
     CDS             complement(190..1140)
                     /locus_tag="PK264_15620"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HRD77335.1"
                     /translation="MALLIIGSQRAGEFARVLGELRPGADLRLWPNAGNLSEITHALA
                     WGPPPGALAQLPNLKLIVSVGAGVDHLFKDPDLPKGVPIVRYVDPDLTRRMVQYVTLH
                     VLWHHRRMCEFQEQQRARVWKYLPEPSPGDVRVGLMGLGVMGAAAVPPLSTLGFKLRG
                     WSRNERHIAGVECFAGDKGFAPFLAETDILVCTLPLTPDTRGVIGKRLLDGLSINGRH
                     ARMPGPAIINAGRGGLQVEGDIMAALASGRLYAASLDVFETEPLPTSSPLWENPRVVI
                     TPHNAAESEPYSIVRYFLRQADAEASGHPLENIVDPKRQY"
     gene            1457..1927
                     /locus_tag="PK264_15625"
     CDS             1457..1927
                     /locus_tag="PK264_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD77336.1"
                     /translation="MPQVKLEVNGKAVTADVDASTLLVDLIRKNLALTGTHVGCDTSQ
                     CGACVVLVNGNSVKSCTMLAAQANGAKVTTIEGLAKGDTLHPVQAAFREAHGLQCGFC
                     TPGMVMSVVDLLAKNPKPSETEIRSWLKGNICRCTGYHNIVKAVQIASGQTPVS"
     gene            1973..>3634
                     /locus_tag="PK264_15630"
     CDS             1973..>3634
                     /locus_tag="PK264_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRD77337.1"
                     /translation="MTGTGIGAAVKRREDAKFLRGIGRYTDDFTFPRETHAFILRSDR
                     AHAKIDLIDTANAVSAEGVVAVFTHADLDADKVNGLPCGWLVTGKGNKPMVEPKHPIL
                     AAGKVRHVGDPVVMIVAETLAAAKNAAEKVIIGYTDLPAVVDMRSALKGGTAVHDEAA
                     DNQCFDWELGDKAATDAAFASAAHVTKLDLVNNRLIPNAMEPRAAIGSFDQASGEHTL
                     VTTSQNPHVIRLLMGAFVLGIPEHKLRVYAPDVGGGFGSKIYHYAEEALVTWGSKKIG
                     RPIRWTAERTESFMSDAHGRDHITHVELAFDKDHKMIGLKADTLANMGAYLSTFAPCI
                     PTWLHATLLAGQYTTPAIHCNVRAVFTNTVPVDAYRGAGRPEATFLLERIVSKAAAEL
                     GVDQCELRRKNFIQPSQFPYQTPVALVYDTGDYEASLNKAIELADYKGFEARRAAAKA
                     KGKLRGIGVASYIEACGIAPSSLVGALGARAGLYEAATVRVNPTGSISVLTGSHSHGQ
                     GHDTTFAQVVADKLGVSIDSIDIVHGDTAKIPFGMGTYGSRSIAVG"
     assembly_gap    3635..3651
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3652..4363
                     /locus_tag="PK264_15635"
     CDS             <3652..4363
                     /locus_tag="PK264_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD77338.1"
                     /translation="TAIVNALDKVIEKGRKIAAHLLEAADSDVVFESGSYKVKGTDKA
                     KAFGEVALAAYVPHKYPIDKIEPGLEETAFYDPKNFTFPAGTYIAEVEIDPRTGETQI
                     IQFAAADDFGNIINPMIVEGQVHGGLVQGIGQALHEAAHYDENGQLKTGSLMDYVMPR
                     ADDVPFFKVATTSTPCTHNPLGVKGCGEAGAIGSPPAIINAICDALREHGVAHIDMPA
                     TPEKIWRAANATMKQAAE"
     gene            4386..5174
                     /locus_tag="PK264_15640"
     CDS             4386..5174
                     /locus_tag="PK264_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRD77339.1"
                     /translation="MYAFDYSRPTSVADAARALETSPGARLMAGGQTLIPTMKQRLAQ
                     PPTVIDLGGVGELRGIHRDGRALGIGAMTTHAEVATSKDVREAIPALAYLASCIGDPA
                     VRHSGTLGGSIANNDPAADYPAAILGLGATVVTNKRTIASDVYFKGLFETSLEGHEIV
                     TAVGFAVPKRAGYAKFEQRASRYALVGVFVAETDGAWRVAVTGAGAKGVFRATDIEKA
                     LASGAAPGSVKIASAGLISDLHGSAEYRAALIPVMAERALAAAK"
     gene            5386..6420
                     /locus_tag="PK264_15645"
     CDS             5386..6420
                     /locus_tag="PK264_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HRD77340.1"
                     /translation="MPNSLLPTTVVGSYPQPEWLIDRTALAKYVPRTRAKSLWRIPEP
                     YLAEAQDDATLLAIRDMERVGLDVITDGEMRRESYSNHFATALEGIDNESPGFVIARS
                     GRTPVPRVVGPVRRARPVEVRDMEFLRRNTDRTTKMTLPGPFTMAQQAQDEHYRDPEA
                     LAMALAEAVNAEAHDLVRAGADVIQLDEPWVRNDPAAARRFAVKAIDRALTGISVPTV
                     VHICFGYGMVVPGESKPNAYAFLEELAASSAGTISIEAAQPNLDLTVLAALAPKSVMV
                     GVLSLADPTIETPEIVAARIRAGLAHIPAERLVPAPDCGMKYLARAVAVGKLDALVKG
                     AAIVRRELEG"
     gene            6563..8206
                     /locus_tag="PK264_15650"
     CDS             6563..8206
                     /locus_tag="PK264_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD77341.1"
                     /translation="MTIGSDLIAKAMKQQGVTDFFYIMGAPMMAVETAAMKLGLRGID
                     VRHEQAGAMAAQAYARLTNKPGFCMAASGPGVTNLLTGTAHAFADCTPVIALGGSAPI
                     ATTNRGAFQDIDQLAMFRSSTKWSDRVVHARRLPEYINLAIRQAMTGKPGPVYLDLPG
                     DVLYAEVDESKVEWPEPYDHARRSRPAGSPEDIKRIVELLAAAKQPVIVTGTGIIWSE
                     AQKELAAFVDAAGVPFYTTPQGRGVIPEDHPYCYLTARSTAFRDADLILVVGTRMNYV
                     IGHAAPPRWNASAKIVRVDIDPGEIAASPRRLDVAITGDAKAVLAQLTAAAKGKLSPE
                     TFRSWRERLRGQNTSKTAEQEAILANASTPIHPLRLCKEIRDFMDRDSILCVDGQEIL
                     NFGRQAIPTFTGSRLNSGAFGTMGVGLPFGLGAKVARPESQVIVLHGDGSFGLNGMEM
                     DTLVRHKLPVLVVISCNGGWTADPKKEKPGRNLGFTRYDRMAEAFGCHGEWVEKPDDI
                     RPALERAMKAIKSGKPALVNVKTDDQARAVTAPFAIYNT"
     gene            8307..8717
                     /locus_tag="PK264_15655"
     CDS             8307..8717
                     /locus_tag="PK264_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77342.1"
                     /translation="MRRFIFAVLILACALATPPLLSTKGRLQAGRNAELPTRTTTLEL
                     VVVEVDQCRHCAHFREAIAPGYRKTWLEKRAPLRFVEAAAFMATSTGLRQPIDTVPTA
                     ILMADGVEVDRVTGLLDPEMFYEAVTFMIERSGV"
     gene            complement(8719..>9787)
                     /locus_tag="PK264_15660"
     CDS             complement(8719..>9787)
                     /locus_tag="PK264_15660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD77343.1"
                     /translation="PVFLFSAAFGQLVFGPMSDRYGRRPVILAGLALYFAGAIAGLVA
                     PSLTVMLGGRMLQGLGSACGIVVARAVLRDSYQGGELARSMAMASGIISIGPVLAPLI
                     GVGLIEIGGWRAVFAGMAAFGALLLASGSLWLAETNRDPNPTAMAPKSLLAAGREVLG
                     HRQSRFFIALAGLNSFMVLSLVTNAPRLFKSSFGLEGLGFATLFAVNGLAVIVGQAIN
                     TRLISRIGPLAATRFAAGTIVAAGALIALLSALDRLNIILFAVLIFAFSTAFPIVMSN
                     SASLTIDPHRRVAGMASSIFGFSSQIVSSILVFATLSLYGGAMLPWAIGLTMAAAITF
                     IALMLYRPPYSADPVRETRSG"
     assembly_gap    9788..9797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1789 a         3639 c         3122 g         1473 t
ORIGIN      
        1 cgttggtcgc ggcgatttcc gccagaaccg cctctgggtt gcgaccctgc cgggcgatca
       61 cctccgccag cgtcatggtg cccgaccgca tgagctcagc cgatctgcgc ccatctggca
      121 caggcgccac cttcgccgcg gcaaggctca cgacccaatc cttcatggca cggctccaag
      181 gctactgcgt caatactgcc gcttcggatc aacgatgttc tcgagtggat gcccggacgc
      241 ctccgcgtcc gcctgccgca ggaaatagcg cacgatcgag tagggctcgc tctcggccgc
      301 gttgtgcggc gtgatcacga cgcgcggatt ctcccagagc gggctcgacg tcggcaaggg
      361 ctccgtctcg aacacgtcga gggaggccgc atagagccgg ccgctcgcca gcgccgccat
      421 gatgtcgccc tcgacctgga gcccgccgcg gcccgcattg atgatcgccg gccccggcat
      481 gcgagcgtgc cggccattga tcgagaggcc gtcgagcagc cgcttgccga tcacgccgcg
      541 cgtatcggga gtcagcggca gcgtgcacac gagaatgtcg gtctcggcga ggaacggtgc
      601 gaagcccttg tcgccggcaa agcactcgac ccccgcgata tgccgctcgt tgcggctcca
      661 gccgcgcagc ttgaagccga gcgttgagag cggcggcacg gccgccgcac ccattacgcc
      721 taggcccatg aggccgactc ggacgtcgcc gggcgagggc tccggcaggt atttccagac
      781 ccgcgcgcgc tgctgctcct ggaactcgca catgcggcgg tgatgccaga gcacatgcag
      841 cgtaacgtac tgcaccatgc ggcgcgtcag atccggatcg acgtagcgca cgatcggcac
      901 acccttcggc aggtcgggat ccttgaagag atgatccaca ccagcgccga cggatacgat
      961 gagcttcagg ttcggcagct gtgcaagagc ccccggcggc ggcccccagg cgagcgcatg
     1021 cgtgatctcg ctgagatttc cggcgttcgg ccacagacgc aggtctgcgc ccgggcgaag
     1081 ctcgccgagt actcgcgcga actcgcccgc gcgctgcgag ccgatgatga gtagtgccat
     1141 gctgctctcc cgccgcgaag ccgcctccgc agccaactcc caatcgcggc ccttcacccc
     1201 gtctcatcgc acaactaaag gcgtgtgcgc cagccttgca aatgccctcg gaaagctctt
     1261 gaatacggtt gccccgatcg tccgctgtca ccgtcagttc ccgggcgaca gtgcggaggc
     1321 cggcggctca gagctatccg tccagcgcca atcttcgccg cacgcgagcc caccagacga
     1381 gttcgagtgc ggcactgccg gccaccccga cgggcagccg acacgcgaga tcgaaataac
     1441 caggaggttc cgcccgatgc cgcaagtgaa actcgaagtg aacgggaagg ccgtcactgc
     1501 cgacgtcgat gcctcgacgc tgctcgtcga tctcatccgc aagaacctcg cgctcaccgg
     1561 cacgcacgtc ggctgcgaca ccagccagtg cggcgcatgc gtcgtcctcg tcaacggcaa
     1621 ttccgtgaaa agctgcacga tgctcgcggc acaggcgaac ggcgccaagg tgacgacaat
     1681 cgagggcctc gccaagggcg acacgctgca tcccgtccag gcggccttcc gcgaggcgca
     1741 cggcctgcag tgcggcttct gcacgcccgg tatggtgatg tcggtcgtcg atctccttgc
     1801 caagaacccg aagccttccg agaccgagat ccgcagctgg ctcaagggca acatctgccg
     1861 ctgcaccggc taccacaaca tcgtcaaggc cgtgcaaatt gcctccggtc agacgcctgt
     1921 cagctgacgc cttccgtgac gacgaccaca atttcctcat aggagcccat tcatgaccgg
     1981 caccggcatc ggcgcagccg taaagcggcg cgaggacgcg aagttcctgc gcggcatcgg
     2041 ccgctacacc gacgacttca cctttccgcg cgagacgcac gccttcatcc tgcgctccga
     2101 ccgcgcgcac gcgaaaatcg acctcatcga caccgcgaac gcggtctcgg ccgagggcgt
     2161 cgtcgccgtg ttcacgcatg ccgacctcga cgccgacaag gtgaacggcc tcccctgcgg
     2221 ctggctcgtc accggcaagg gcaacaagcc catggtcgag cccaagcacc cgatcctcgc
     2281 cgccggcaag gtgcgccacg tcggcgaccc cgtcgtcatg atcgtcgctg aaacactcgc
     2341 cgccgcgaaa aacgcggccg agaaggtcat catcggctac acggaccttc ccgccgtcgt
     2401 cgacatgcgc tcagccttga agggtggcac cgccgtccat gacgaggccg ccgacaacca
     2461 gtgcttcgac tgggagctcg gcgacaaggc cgcgaccgat gccgccttcg ccagtgccgc
     2521 tcatgtgacg aagctcgacc tcgtcaacaa ccgcctcatc ccgaacgcca tggagccgcg
     2581 cgccgcgatt ggctcgttcg accaggcgag cggcgaacac acgctcgtga cgacgagcca
     2641 gaacccgcat gtcatccgcc tgctgatggg cgccttcgtg ctcggcattc ccgagcataa
     2701 gcttcgcgtc tatgcgcccg atgtcggcgg cggcttcgga tcgaagatct accattacgc
     2761 cgaggaagct ctcgtcactt ggggctcgaa gaagatcggc cgcccgatcc gctggaccgc
     2821 ggagcgcacc gagagtttca tgtcggatgc ccacggccgc gatcacatca cccacgtcga
     2881 gctcgccttc gacaaggacc acaagatgat cggcctcaag gccgacacgc tcgcaaacat
     2941 gggcgcctat ctctcgacct tcgcgccctg catcccgacc tggctgcacg cgacgctgct
     3001 cgccggccag tacacgacgc cggcgatcca ctgcaacgtg cgcgccgtgt tcacgaatac
     3061 ggtgccagtc gacgcttacc gcggcgccgg ccgccccgag gcgactttcc tcctcgagcg
     3121 catcgtctcg aaggccgcgg ccgagcttgg cgtcgaccag tgcgagctgc gccgtaagaa
     3181 ctttatccag ccgagccagt tcccctacca gacgcctgta gccctcgttt atgacacggg
     3241 cgactacgag gcgagcctca acaaggcgat cgagctcgcc gactacaagg gcttcgaggc
     3301 gcgccgcgcg gccgccaagg cgaagggcaa gctccgcggc atcggcgtcg cgagctacat
     3361 cgaggcgtgc ggcatcgcac cctcgagcct cgtcggcgcc ctcggcgccc gtgccggcct
     3421 ctacgaggcc gcgaccgtgc gcgtgaaccc gaccggctcc atctcggtgc tgacgggctc
     3481 gcacagccac ggtcagggcc acgacaccac gttcgcccag gtcgtcgccg acaagctcgg
     3541 cgtctcgatc gacagcattg acatcgtgca cggcgacacc gccaagatcc cgttcggcat
     3601 gggcacctac ggctcgcgct cgatcgcggt cggcnnnnnn nnnnnnnnnn ncacggcgat
     3661 cgtcaacgcg ctcgacaagg tgatcgagaa gggccgcaag atcgctgccc atcttctcga
     3721 agctgccgac agcgacgtcg tcttcgagag cggctcgtac aaggtcaaag gcaccgacaa
     3781 ggccaaggcc ttcggcgagg tagcacttgc cgcctacgtg ccgcacaagt acccgatcga
     3841 caagatcgag ccgggtctcg aggagacggc cttctacgat ccgaagaact tcacgttccc
     3901 ggccggcacc tacatcgccg aggtcgagat cgatccgcgc acgggtgaga cccagatcat
     3961 ccagttcgcc gccgccgacg acttcggcaa catcatcaac ccgatgatcg tcgaggggca
     4021 ggtgcacggt ggactcgtcc agggcatcgg ccaggcgctg cacgaggccg cgcactacga
     4081 cgagaacggg cagctcaaga ccggcagcct catggactac gtgatgccgc gcgccgacga
     4141 cgtgccgttc ttcaaggtcg cgaccacctc gacgccctgc acgcacaatc cactcggcgt
     4201 gaagggctgc ggcgaggccg gcgctatcgg ctcgccgcct gccatcatca acgccatctg
     4261 cgacgcgctc cgcgagcacg gcgtcgccca catcgacatg ccggcaacgc ccgagaagat
     4321 ctggcgcgcc gccaacgcca ccatgaaaca ggccgcggaa taagaccgcg aagggagatc
     4381 cacccatgta cgcattcgac tacagccggc cgacctcggt cgccgatgcg gcccgcgccc
     4441 tcgagacgtc gcccggcgca cgtctcatgg caggcggcca gacgctgatc cccaccatga
     4501 agcagcgcct tgcccagccg ccgactgtca tcgacctcgg cggcgtcggc gagctcaggg
     4561 gcatccaccg cgatggccgt gccctcggca tcggcgcgat gaccacgcac gccgaggtcg
     4621 ccacatcgaa ggacgtgcgc gaggcgattc ccgcgcttgc ctacctcgcg agctgcatcg
     4681 gcgatccggc cgtgcgccat tccggcacgc tcggcggctc gatcgccaac aacgatccgg
     4741 cggccgacta tccggccgcg atcctggggc tcggtgcgac cgtcgtcact aacaagcgca
     4801 cgatcgcatc cgacgtgtac ttcaagggcc tgttcgagac ctcgctcgaa ggccacgaga
     4861 tcgtgacggc cgtcggcttt gccgtcccga agcgcgccgg ttacgccaaa ttcgagcagc
     4921 gcgcctcacg ctatgccctc gtcggcgtgt tcgtcgccga gacggacggc gcctggcgcg
     4981 tcgcggtcac gggcgccggt gccaagggcg tgttccgcgc gaccgacatc gagaaggcgc
     5041 tcgcctcggg cgccgctccc ggcagcgtga agatcgcgtc ggccggcctc atctcggacc
     5101 tgcacggcag tgccgaatac cgcgctgccc tcatcccggt catggccgag cgggcgcttg
     5161 ccgctgcgaa gtaagccggt cgtagcattt cggcggcccg gccatgcgcc gggccgtttc
     5221 gcgtcgcgag cggtgctttc gccccgcgac ccagttggtg ggaccccctc caaaccaccc
     5281 gtcttttggc ctgcagacaa gacgcggcag ggttcgggcc gccccgcgcg cggcagaggg
     5341 caagtcgccg cgcgaactca ccctctcaga ccacgtggag ccatcatgcc gaactcgctc
     5401 ctcccaacca ctgtcgtcgg cagctacccg cagccggaat ggctcatcga ccgcaccgcg
     5461 ctcgcgaagt acgtgccgcg cacgcgcgcg aagtcgctgt ggcgcatccc agagccgtac
     5521 ctcgccgagg cgcaggacga tgcaacgctt ctcgccatcc gcgacatgga gcgcgtcggc
     5581 ctcgacgtga tcaccgacgg cgagatgcga cgcgagagtt actccaacca cttcgcgacc
     5641 gcgctcgagg gtatcgacaa cgagagccct ggcttcgtca tcgcacgctc aggccgcacc
     5701 cccgtcccgc gcgtcgttgg cccggtccgt cgcgcgcgcc cggtcgaggt ccgcgatatg
     5761 gagttcctgc gccgcaatac cgaccgaacg acaaagatga cgctgcccgg cccattcaca
     5821 atggcgcagc aggctcagga cgagcactat cgcgaccccg aggcgctcgc catggccctg
     5881 gccgaggccg tcaacgccga ggcgcacgat ctcgtccgcg ccggcgcgga cgtgatccaa
     5941 ctcgacgagc cctgggtgcg caatgatccc gccgccgccc gccgctttgc cgtgaaggcg
     6001 atcgaccgcg cgctcaccgg catcagtgtg ccgacggtcg tccacatctg cttcggctac
     6061 ggcatggtgg tgccaggcga atccaagccc aacgcctacg ccttcctgga ggagctcgcg
     6121 gcaagctctg ccggcacgat ctcgatcgaa gccgcacaac cgaatctcga cctcacggtg
     6181 ctcgccgcgc tcgctccgaa atccgtgatg gtcggcgtgc tcagcctggc cgatccgacc
     6241 atcgagacac ccgagatcgt cgccgcgcgc attcgcgcag ggcttgcgca catccccgcc
     6301 gagcgcctag tccccgcgcc cgactgcggt atgaagtatc tcgcccgcgc cgttgctgtc
     6361 ggcaagctcg acgccctcgt caagggcgcc gccatcgtcc gccgcgaact tgagggctaa
     6421 gccctgctcc ccgatcccag cgagccgcgc attgctgccg accggcaacg ggcgagcctc
     6481 gacgcttgag ccgcgccacc gtgcggggca ggatgctgtc gcccaaccgg cccggcacgg
     6541 ccaccgaacc agaggacacg ccatgaccat cggctccgac ctcatcgcca aggcgatgaa
     6601 gcagcagggc gtcaccgact tcttctacat catgggcgcg ccgatgatgg cggtcgagac
     6661 ggccgcgatg aagctcgggc tccgcggcat cgatgtgcgc cacgagcagg cgggcgccat
     6721 ggccgcgcaa gcctatgccc gcctcaccaa caagccgggc ttctgcatgg cggcctcggg
     6781 ccccggcgtg acaaacctcc tgaccggcac cgcgcacgcc ttcgccgact gcacgccggt
     6841 gatcgcgctc ggcggctcgg cgcccattgc gacgacgaac cgcggcgcct tccaggacat
     6901 cgaccagctc gcaatgttcc gctcctcgac caaatggtcc gaccgcgtcg tgcatgcccg
     6961 gcgcctcccc gagtacatca acctcgccat ccgccaggcg atgacgggga agcccgggcc
     7021 cgtctacctc gacctcccgg gcgacgtgct ctacgccgag gtcgacgaga gcaaggtcga
     7081 gtggcctgag ccctacgacc acgcgcgccg cagccgtccc gcaggctccc ccgaggacat
     7141 caagcgcatc gtcgagctgc tcgccgcggc gaagcagccc gtgatcgtca ccggcacagg
     7201 catcatctgg tcggaggcac agaaggagct cgcggctttc gtcgatgccg cgggcgtgcc
     7261 cttctacacg acgccgcaag ggcgcggcgt catccccgag gatcacccct actgctacct
     7321 gaccgcgcgc tcgaccgcct tccgcgacgc cgacctcatt ctcgtcgtcg gcacacgcat
     7381 gaactacgtc atcggccatg ccgcgccgcc acgctggaac gccagcgcca agatcgtccg
     7441 cgtcgatatc gacccgggcg agatcgcggc gagcccgcgc cgtctcgacg ttgccatcac
     7501 tggtgacgcc aaggccgtgc tcgcgcagct gaccgccgcc gccaagggta agctcagccc
     7561 cgaaacattc cgctcctggc gcgagcgtct gcgcgggcag aacacctcca agaccgccga
     7621 gcaggaggcg atactcgcca acgcctcgac gccgatccac ccgctgcgcc tctgcaagga
     7681 gatccgcgat ttcatggatc gcgattcgat cctgtgcgtc gacggccagg agatcctgaa
     7741 cttcggccgc caggcgatcc cgaccttcac ggggagccgc ctcaattccg gcgccttcgg
     7801 cacgatgggc gtcggccttc cttttggtct cggcgcgaaa gtcgcccgcc cggaaagcca
     7861 agtgatcgtg ctgcacggcg atggctcgtt cggcttgaac ggcatggaaa tggacaccct
     7921 cgtccgccac aagctcccgg tgctcgtcgt catcagctgc aatggcggct ggaccgccga
     7981 ccccaagaag gagaagcccg gccgcaacct cggcttcacc cgatacgatc gcatggccga
     8041 ggccttcggc tgccacggcg aatgggtcga gaagccggac gacatccgcc ctgcccttga
     8101 gcgcgccatg aaagcgatca agtctggcaa gcccgcgctc gtcaatgtga agaccgacga
     8161 tcaggcccgc gcggtgacgg cgccgttcgc catttacaat acctgatgcg ttaacagcca
     8221 atcccgaccg atcggcacgc ctgacgcacg ctggcatgaa ccttgcgccg tccgcgcgag
     8281 gttcaacgcc agagtgcttc tcgcccatgc gtcgcttcat ttttgccgtt ctcattctcg
     8341 cgtgtgcgct tgccacgccg cccctgctca gcacgaaggg gagactccaa gccggccgca
     8401 acgccgagct tcccacgcgc acgacgacgc tcgagctcgt cgtcgtcgag gtcgaccagt
     8461 gccgccactg cgcccatttc cgcgaggcga tcgctccggg ctaccgcaag acctggctcg
     8521 agaagcgcgc gccgctgcgc ttcgtcgagg ccgcagcctt catggcgacc agcacgggcc
     8581 tccgccagcc gatcgacacc gtgccgaccg caatcctgat ggccgacggc gtcgaggtcg
     8641 atcgcgtgac cggccttctc gacccagaga tgttttacga ggccgtcacc ttcatgatcg
     8701 agcgctccgg cgtgtagctc atccgctccg cgtctcgcgc actggatcgg ctgaataggg
     8761 cggccggtag agcatcagcg caatgaatgt gatcgctgcc gccatcgtca ggccgatcgc
     8821 ccacggcagc atcgcgccgc catagaggga gagcgtcgcg aagacgagga tgctcgagac
     8881 gatctgcgac gagaagccga agatcgacga ggccatgccg gcgacgcgcc ggtgcggatc
     8941 gatcgtcagg ctcgccgagt tcgacatgac gatcgggaag gcggtgctga aggcgaatat
     9001 cagaacggcg aacaggatga tgttgagacg gtccaaggcc gagagcagcg cgatgagcgc
     9061 gcctgccgca acgatcgtac ccgccgcaaa acgtgtcgct gccagcggcc cgatgcgcga
     9121 gatgagccgc gtgttgattg cctgaccgac gatgaccgcg aggccattca cggcaaagag
     9181 cgtcgcaaag ccgagcccct cgagtccgaa cgacgacttg aagagtcgtg gcgcgttcgt
     9241 aaccagcgag agcaccatga aggagttgag ccccgcgagc gcgatgaaga agcgcgactg
     9301 acgatggccg agcacctcgc gcccggcggc aagaaggctt ttcggcgcca tggcggtcgg
     9361 gttcggatcg cggttggtct cggcgagcca gaggctgccg ctcgcgagca ggagtgcgcc
     9421 gaatgccgcc attccggcga acaccgcgcg ccaaccgccg atctcgatga gcccgacgcc
     9481 gatcagcggc gccagcaccg gtccgattga gatgatgccg ctcgccattg ccatcgagcg
     9541 ggcaagctcg ccgccctggt agctgtcgcg cagcacggct cgtgcgacca cgatcccgca
     9601 ggcgctgccg agcccctgca gcatgcgccc gccgagcatg accgtgagac tcggcgcgac
     9661 gaggcccgcg atcgcgcccg cgaaatagag cgcgagaccg gcgaggatca cggggcgacg
     9721 gccatacctg tccgacatcg gcccgaatac gagctgtccg aaggcggcgg agaacaggaa
     9781 caccgggnnn nnnnnnnaac tgcacgcgct cgatcggcgc gtcgaggccg cgtccgatcg
     9841 cccagaaggc cggcagcgtg atgtcgcagg agaacgcgtt cgcggcgagc agcaggccgc
     9901 agaacgcaat caggaaggtg cgggtcatgc ggtgtcctgg ttgcctccgg ccgcaatgca
     9961 gcaatcgggc tcgcgcggca agccccgcgc cggcacggcc ctcatgccga tccgtgtggg
    10021 acggttgcgc tcagcgcttc gcggcagcca
//
LOCUS       DAORNH010000163        10018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5001_length_10018_cov_2.834488, whole genome shotgun sequence.
ACCESSION   DAORNH010000163 DAORNH010000000
VERSION     DAORNH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5001_length_10018_cov_2.834488"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PK264_15665"
     CDS             complement(<1..688)
                     /locus_tag="PK264_15665"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD77344.1"
                     /translation="MPEIRPRRSMLYMPGSNARAIEKARSIDADAIILDLEDAVAPEA
                     KVEARGRIVASLEAGGFGPREMAVRINGLDTPWGADDLAAVVKAKPAAILLPKVQSRA
                     EILRASEAMTRAGAGADMQLWGMMETPVAILKAAEIAAAGAADGSRLKLLLMGTNDLI
                     KDTRAEMTADRTAAMYWLQACLTAARAYGLDVLDGVYNNFKDAEGYKRECVHGRALGF
                     DGKTLIHPDQV"
     gene            793..1710
                     /locus_tag="PK264_15670"
     CDS             793..1710
                     /locus_tag="PK264_15670"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HRD77345.1"
                     /translation="MAFKLALDRDLEADARRALEAQVEVIGVRLKDADPDSAVHEARK
                     GIKRMRSLLRLIDSGLSPKTFESLNGLLRDIARGLAAARDEAALRAASADLTAANEGQ
                     TKLVADLERIAAALSTSRASGDLAAERRAARRGLAAVKRRLAKLSVAGDRIVVLSDGL
                     SATYRRGRKALKVARASGNDDDYHELRKHVQAHWRQMKLVIEAWPEEFAARLATAQVL
                     SANLGLDHDLSLLVQYIAADAKSDLSVSAAERVTSACRARQMVLRTTAARALVHLYCE
                     RTSAFRARMHAYLAAHDLTGLDDAAPPID"
     gene            1688..2740
                     /locus_tag="PK264_15675"
     CDS             1688..2740
                     /locus_tag="PK264_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HRD77346.1"
                     /translation="MPRPPSTNATTTEPGKLNLITDVPGILVGHAADTRAASGVTAIL
                     FGTPDRTATVAAVTRGGAPGTRDTTLLEPEMTVAGIHAVVLSGGSLFGLDAAGGVVSH
                     LRKAGIGLRIGGAVVPIASQAICFDLLNGGDKDWGRVPPYWELGWQAASSARGGSFAL
                     GTVGGGYGATTATLKGGLGSASITTASGYTVGALAIVNAVGSATIGDGPHFWAAPLEQ
                     DGEFGGRGMPHAFSGSDLAPRLKGGAPPSTTIALVATDARLSKAEAKRIAIMADDGLA
                     RALRPAHAPMDGDTVFAAATGLRILDQPAVHLTEIGMAAGDCLARAIARGVYEATALP
                     FAGALPTWREKFGTTN"
     gene            3078..3815
                     /gene="pyrH"
                     /locus_tag="PK264_15680"
     CDS             3078..3815
                     /gene="pyrH"
                     /locus_tag="PK264_15680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRD77347.1"
                     /translation="MSPAARKPFKRLLLKLSGEALMGKSGYGIDTAVVARLAADVKEV
                     QATGREIAIVVGGGNIFRGLAAATKGMDRATADYMGMLGTVMNSLAFQGALEGIGVPS
                     RVLSAIPMPTVCEAYVRPKALHHMAAGRIVIFAAGTGNPFFTTDTAAALRAIEMGCDV
                     LAKATQVDGVYSADPKKDPSATRFDQLTYTEVLGRNLQVMDGAAIALARDNRLPVIVF
                     SINEPGNLMRVLEGKARSTLVTEDPKA"
     gene            3895..4458
                     /gene="frr"
                     /locus_tag="PK264_15685"
     CDS             3895..4458
                     /gene="frr"
                     /locus_tag="PK264_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRD77348.1"
                     /translation="MAAATFDIKDLEKRMRGPLDNLKREFSGLRTGRASAALLEPVRV
                     NVYGQKMPLNQVATVSTPDARTISVQVWDRTNITPVEKAIREANLGINPVVDGTLIRL
                     PIPPLTGDRRADLVKQAHKYAEAARIGVRNVRREGMELLKKLEKDHKITEDDHRKNQT
                     KVQELTDKLIKEIDQGLTTKEAEIKTV"
     gene            4496..5284
                     /locus_tag="PK264_15690"
     CDS             4496..5284
                     /locus_tag="PK264_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRD77349.1"
                     /translation="MTQRPMASGGTVASTARSEAATAAVSEPAIGADPRHVAIIMDGN
                     GRWAKLRGLPRAEGHRRGVEAVRRVVRAAIELKIPFLTIYSFSSENWSRPAAEVDDLM
                     GLMKRFIRQDLVDLHQNGVRLRVIGERDRVDPDLLHLFDEAVELTSTNTKLQLVVAFN
                     YGARTEIARAAKRLAERARAGTLDPASITAELLSHELDTRDIPDPDLLIRTSGEVRLS
                     NFLLWQAAYSELIFVDTFWPDFDRAELESAVATYRQRQRRFGGV"
     gene            complement(5371..5547)
                     /locus_tag="PK264_15695"
     CDS             complement(5371..5547)
                     /locus_tag="PK264_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77350.1"
                     /translation="MLGIGILLLILGAGSYVLPMMGMQFKLMSIFGAYQGWAQIGAIV
                     VGAILVVMSLLGRK"
     gene            complement(5659..6420)
                     /locus_tag="PK264_15700"
     CDS             complement(5659..6420)
                     /locus_tag="PK264_15700"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRD77351.1"
                     /translation="MPTYLHDAQTPREPDPETGLPIGPRVTPGGPARRPERVVLDGRY
                     CRLEPLEADRHADELFLASTPPDAVARFQYLPVAAPATRADFDAWVASSATAVDRLYF
                     AVVDKRSGAVAGRQSLMRIDPANRSIEIGDIYWGPAIARSPVTTEANFLFARYAFDTL
                     GYWRYEWKCNALNGPSRQSAERFGFTYEGLFRRSTIVRGRTRDTTWFAMIAEEWPALR
                     TAYETWLAPENFDGHGGQRQRLSDLTRAALAETRR"
     gene            complement(6524..6814)
                     /locus_tag="PK264_15705"
     CDS             complement(6524..6814)
                     /locus_tag="PK264_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HRD77352.1"
                     /translation="MSSLQASAQGQLRQLVEQIERLEEEKKNIAGDVRDKFAEAKALG
                     FDVKVLRQVIRLRKKSKTDRLEEEAVLDTYLHALGMLDDASAAAPPMMDAAE"
     gene            complement(6976..>7306)
                     /locus_tag="PK264_15710"
     CDS             complement(6976..>7306)
                     /locus_tag="PK264_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRD77353.1"
                     /translation="IGVDCSGLVQLALEAAGLKAPRDSDMQQAALGENVLIPADLDGL
                     KRGDLVFWPGHVAVMTDGMMLLHANAHHMSAVSEPLTDAVTRIARAGSTISAIKRLPA
                     LSAKGAA"
     assembly_gap    7307..7316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7317..7873)
                     /locus_tag="PK264_15715"
     CDS             complement(<7317..7873)
                     /locus_tag="PK264_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRD77354.1"
                     /translation="MAAGGSHRLDRRVNAYRADLAAAELAGMVEAERFTTGAEAQVVR
                     GSVAVRAAPTSGEGLLTEALFGERVTVFEEQGGWAWVQLARDRYVGYVPADALTRSTR
                     ETGHRVKALGTFLYSEPDIKSPPLMHLSLNSELSVSEAGEKFLALASGGYVIARHVAE
                     SHRHASDFVDIAERFAGTPYLWGGRT"
     gene            complement(7939..9378)
                     /locus_tag="PK264_15720"
     CDS             complement(7939..9378)
                     /locus_tag="PK264_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HRD77355.1"
                     /translation="MTTREKSWPQSPADIIIDGATKRQTRPIWLVREGREGDVLPALS
                     GEQRAWLKSHGFKGTARRFLLLPSPEGGIAGAVLGIGPPQAGKGEPIALLAGLLPTLL
                     PPGGYHLETDLGADAELAATAYGLGAYRFRRYKSAEADPLAVLKTPRGVNRENVLSAI
                     EAVWIGRDLINTPANDMGPAELEAAARDLARRHDAQASVIIGDDLLARNFPLIHTVGR
                     ASPRAPRLIELTWGRADAPRVTLVGKGICFDTGGLDIKPSASMLLMKKDMGGAASVLA
                     LAHMIMARRLDVRLRIVIAAAENSVAGNAFRPGDVLKSRGGRTVEIGNTDAEGRLVLA
                     DALALADEDTPDVLMTFATLTGAARVALGPDLPPVFCDDDATATALAAAGEAVGDPMW
                     RLPMTTPYESWLDSPVADMNNVAEGGFAGSIVGAMFLKRFVKRAKRFAHFDIYGWRPA
                     AKPIGPKGGEPQGARAVFEWLKRTHGRTV"
     gene            9500..>10018
                     /locus_tag="PK264_15725"
     CDS             9500..>10018
                     /locus_tag="PK264_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD77356.1"
                     /translation="MVAQQYRLVIGSKNWSSWSLRPWIALKRFSIPFDETNIDLRAAD
                     RSQQIAASSPSAKVPVLLIDGEPVWESLAILETLADRHPEHAFWPKAAPARAMARAVA
                     NEMHAGFADLRQHCPMDFLGRAPMASLPDPVAANVRRIVKVWGEARVRHGAGGPFLFG
                     AFSIADAMYAPVV"
BASE COUNT         1696 a         3490 c         3221 g         1601 t
ORIGIN      
        1 ccacctggtc ggggtggatc agcgtcttgc cgtcgaaacc gagcgcccgg ccgtgcacgc
       61 actcgcgctt gtagccctcg gcatccttga agttgttgta gacgccgtcg aggacgtcga
      121 ggccgtaggc gcgcgctgcg gtgaggcagg cctggagcca gtacatggcg gccgttcgat
      181 ctgccgtcat ctcggcgcgc gtgtccttga tgaggtcgtt ggtccccatc agcaggagct
      241 tgaggcgcga gccgtcggcg gcccctgccg cggcgatctc cgccgccttc aggatcgcga
      301 ccggtgtctc catcatgccc cagagctgca tgtcggcacc cgccccggct cgtgtcatgg
      361 cctcggacgc ccgcaggatc tcggctcgcg actggacctt cggcagcagg atcgccgccg
      421 gcttcgcctt caccaccgcc gcaagatcgt cggctcccca cggcgtgtcg agcccgttga
      481 tgcggaccgc catctcgcgc ggaccgaagc caccggcctc aaggctcgcg acgatgcgcc
      541 cgcgcgcctc gaccttggcc tcgggcgcca cggcgtcctc gaggtccagg atgatcgcat
      601 cggcgtcaat cgaacgcgcc ttctcgatgg ctcgggcgtt cgagcctggc atgtaaagca
      661 tgctgcggcg cgggcggatc tctggcatgg gcacctcttc tgacagatac cggctttgag
      721 cgcgtttcaa gcctttgatc aagccggttg cggtgtcaca catcgcgccg cgggaagtgc
      781 tagcattccg ccatggcctt caagcttgcc ctcgatcgtg acctcgaagc agatgcccgc
      841 cgcgccctcg aggcgcaggt ggaggtcatc ggcgtgcggc tcaaggacgc cgaccccgat
      901 tccgccgtgc atgaggcgcg caaggggatc aagcgcatgc ggtcgctgct gcgcctgatt
      961 gacagcgggt taagcccgaa gactttcgaa agcctgaacg ggctcctgcg tgacatcgcg
     1021 cgcggcctag cggcggcgcg tgacgaggca gccctgcgcg cggcctcagc cgatctcacc
     1081 gcggccaacg agggtcaaac gaagctcgtc gccgatctcg agcgcatcgc ggcggcactg
     1141 tcgacgagcc gagcctcggg cgatctcgca gcggagcggc gcgccgccag gcggggcctc
     1201 gcggcggtta agcgccgtct ggcgaagctc tctgttgcgg gtgaccggat cgttgtcctc
     1261 tccgacggtc tctccgccac ataccggcgt gggcgcaagg ccttgaaggt tgcaagggcg
     1321 agtggcaacg acgacgacta tcatgagttg cgcaagcacg tacaggcgca ctggcggcaa
     1381 atgaagctcg tcatcgaggc ctggcccgag gaatttgctg cccgcctcgc gaccgcacaa
     1441 gtcctctccg ccaatctcgg cctcgatcat gacctgagcc tgctcgtcca gtacattgcg
     1501 gccgacgcca agtcggattt gagcgtcagc gcggccgagc gtgtgacctc ggcatgccgg
     1561 gcccgccaaa tggtgctgag aaccactgcc gcgcgggcgc tcgtccacct ctactgcgag
     1621 cgcacatcgg catttcgcgc ccgcatgcac gcctatcttg ccgcacacga cctgacaggc
     1681 ctcgacgatg ccgcgccccc catcgactaa cgcaacaacg accgagccag gcaagctgaa
     1741 cctcatcacc gacgtgccgg gcatcctcgt cggccatgca gcggacacgc gcgcagcgag
     1801 cggggtcacc gcgatcctct tcggcacgcc cgaccggacg gcgaccgtcg ccgccgtcac
     1861 ccgcgggggc gcgcccggta ctcgcgatac gaccttgctc gagccggaaa tgaccgtcgc
     1921 cggcattcat gcggttgtcc tttcgggcgg ctcgctgttc ggcctcgacg cagccggcgg
     1981 cgtggtcagc cacctgcgca aggcgggcat cggcctccgg atcggcggtg ccgtggtgcc
     2041 gatcgcctct caggccatct gcttcgatct gctcaacggc ggcgacaagg actggggacg
     2101 tgtgccgccc tactgggagc tcggctggca ggcggcctcc agcgcccgcg gcgggtcatt
     2161 cgcactcggc accgtcggcg gaggctacgg cgcgacgacc gcaacgctca aaggcggcct
     2221 aggctcggcg agcatcacga cggcgagcgg ctacaccgta ggcgcgctgg ccattgtcaa
     2281 tgctgtcgga agcgcgacga tcggcgatgg cccgcacttc tgggcggcac ctttagagca
     2341 ggacggcgaa ttcggcggcc gcggcatgcc gcacgcattt tccggcagtg atctcgcgcc
     2401 acgcctgaag ggcggagcgc cgccgtcgac gaccatcgca cttgtcgcga cggatgcgcg
     2461 cctcagcaag gcggaggcaa agcgcatcgc catcatggcc gacgacggtc tcgcgcgcgc
     2521 actccgccct gcccacgctc ccatggacgg cgatacggtg tttgctgcag caaccgggct
     2581 gcgcatcctc gatcaacctg ccgtccatct gaccgagatc ggcatggccg ccggcgactg
     2641 cctcgcccgc gccatcgcgc gtggggtcta cgaggcgact gcactcccat tcgcgggagc
     2701 gctccccacg tggcgggaga aattcgggac gaccaactga ggcatgatcc cgcacatcgg
     2761 tcctcgccga ctgtggtgcc ggaccgatat ccggcgatcg catggcgcag cacgaccggg
     2821 tgacggcccc acaggctcgc gtcgcgccgc ttccattgag tgaggatttg actgtgcagc
     2881 gtcctgtcga tccaaagtcg tcctgcgccc cgctcgaatc tcgcccgatg agggagttca
     2941 gggaagcgca acgctcggct gacggttaag cgatccccgt cagacccgcg ccccacgcca
     3001 gccgctcgtg gcgcactggt gcgagcacgc gcgatcggct agacctcggc ccgatcgatc
     3061 cagacgacga ggaccgcatg tcgcccgcag cacgcaagcc gttcaagcgg ctccttctga
     3121 agctttcggg tgaagccctg atgggcaaga gcggctatgg catcgatacc gccgtcgtcg
     3181 cccgcctcgc tgccgacgtg aaggaggtgc aggccacggg gcgcgagatc gccatcgtcg
     3241 tcggcggcgg caacatcttc cgcggcctcg ccgccgcgac caagggcatg gaccgggcca
     3301 ccgccgacta catgggcatg ctcggcaccg tcatgaactc gctggccttc caaggcgccc
     3361 tcgaagggat cggcgtcccc tcccgcgtgc tctcggcgat ccccatgccg acggtctgcg
     3421 aagcctatgt ccgccctaag gcgctgcatc acatggccgc cggccgcatc gtgatcttcg
     3481 ctgccggcac gggcaacccc ttcttcacga cggatacggc cgcggccttg cgggcgatcg
     3541 agatgggctg cgatgtcctc gccaaggcga cgcaggtcga cggtgtctac tccgccgacc
     3601 cgaagaagga cccgagcgcc acacgctttg atcaactcac ctacacggag gtgctcggcc
     3661 gcaacctcca ggtcatggac ggcgccgcga tcgcccttgc ccgtgacaat cgacttccgg
     3721 taattgtgtt ctcgatcaac gagccgggaa acctgatgcg ggtgctcgag ggcaaggcac
     3781 gctcgacact tgtcaccgag gatccgaagg cgtgacggac agcgcctgaa gcgagacgac
     3841 ctcgcgtctg gcgtcagtgg cgcacgacag ctcagcatgt tcatggaggc agccatggcc
     3901 gcggcgacat tcgacatcaa ggacctcgag aagcgcatgc gggggcccct cgacaacctg
     3961 aagcgcgagt tttcggggct gcgcacgggc cgcgcctcgg cagcactcct cgagccggta
     4021 cgcgtcaacg tctacggtca gaaaatgccg ctcaaccagg tggcgaccgt ctcgacaccc
     4081 gatgcccgca cgatctcggt gcaggtgtgg gaccgcacca acatcacgcc ggtcgagaag
     4141 gccattcgtg aggcgaacct cggcatcaac cccgtggtcg acggcacgct catccggctc
     4201 cccattccgc cactcacggg cgaccggcgc gccgacctcg tcaaacaggc tcacaaatat
     4261 gccgaggcgg cgcggatcgg cgtccgcaac gtcaggcgcg agggcatgga gcttctcaag
     4321 aaactcgaga aggaccacaa gatcaccgag gacgatcacc gcaagaacca gacgaaggtt
     4381 caggaactga cggacaagct catcaaggag atcgaccagg ggctcaccac caaagaggcc
     4441 gagatcaaaa cggtctgaag gggttttgac actctccccg gtgaggcgcg cagacgtgac
     4501 acagcgaccg atggcatccg gcggtacggt ggcctccacc gcgaggagtg aggcggccac
     4561 cgccgcagtc agtgagccgg caatcggcgc cgatccgcgt cacgtcgcga tcatcatgga
     4621 cggcaatggc cgctgggcga agctgcgcgg cctgccacgg gcggaaggtc atcgccgcgg
     4681 cgtcgaagcg gtgcggcgcg tagtgcgggc ggcgatcgag ctgaaaatcc cgttcctgac
     4741 gatctacagc ttctcgtcgg agaactggtc acgccccgcc gccgaggtcg acgacctgat
     4801 gggactcatg aagcggttca tccggcaaga ccttgtcgat ctccaccaga acggcgtacg
     4861 cctccgcgtc atcggcgagc gcgatcgggt cgatcccgat ctgctccacc tattcgacga
     4921 ggcagtcgag ctcacctcga cgaacaccaa gctgcagctc gtcgtcgcct tcaactatgg
     4981 ggcgcggacc gagatcgcgc gcgcagccaa gcgactcgcc gagcgcgcca gagcaggcac
     5041 gctcgatccg gcgagcatca cggcagaatt gctgtcacac gaactcgaca cgcgcgacat
     5101 accggacccc gatctcctga tccgcaccag tggcgaggtc cggctctcaa atttcctgct
     5161 ctggcaggcc gcctacagcg agttgatatt cgtcgacacc ttctggccgg acttcgaccg
     5221 tgcggagttg gagagcgccg ttgcgaccta ccgccaacgt cagcgccggt tcggcggcgt
     5281 gtgacacgcc gcgctgcaaa gaccagcgcc ctttggcaac tggccacctg ctgtctctca
     5341 cgacgccaac gagcgtcgat gagccggccc ctacttccgc ccgagcaggc tcatgacgac
     5401 caggatcgcg ccaaccacga ttgcgcctat ctgcgcccag ccctgatagg cgccgaagat
     5461 gctcatcaac ttgaactgca tgcccatcat cggtaggaca taggagccgg cgcccaggat
     5521 gagcagaagg attccaatgc caagcatgag cgtcccctat cgttgcctgc gatgattcgc
     5581 ggcgccagca tgcgggctcg accgatcacc ggtcaactgg gttgcatcgg cgctgcgaaa
     5641 gggacccctg ccgaggcgct accgacgggt ctccgccaat gccgcacgcg taaggtcgct
     5701 gaggcgctga cgctggccac catgcccgtc gaaattctcg ggcgcgagcc acgtctcata
     5761 ggcggtccgc agtgccggcc attcctcggc gatcatggcg aaccacgtcg tatcccgggt
     5821 tcggccccgt acgatggttg aacgccggaa cagtccctcg taggtgaacc cgaagcgctc
     5881 ggccgactgg cgcgagggcc cgttcagcgc attgcatttc cattcgtagc gccagtagcc
     5941 gagcgtgtcg aaggcatagc gtgcgaacag gaagttggcc tcggtcgtca ccggtgacct
     6001 cgcgatggcc ggcccccaat agatgtcgcc gatctcgatg ctccggttcg cgggatcaat
     6061 ccgcatcagg gactgccggc cggcgaccgc acccgagcgc ttgtcgacca cggcgaagta
     6121 gagccggtcc acggccgtgg ccgagcttgc gacccaggcg tcgaagtcgg cacgtgtggc
     6181 tggagccgct acaggaagat attggaagcg cgcgaccgcg tccggcggcg tcgaggcgag
     6241 gaacagctcg tcggcatggc gatccgcctc gagcggctcg aggcggcaat agcgtccgtc
     6301 gagcacgaca cgctcagggc ggcgcgccgg tccacctggc gtaacgcggg gcccgatcgg
     6361 cagcccagtc tcgggatcgg gctcacgggg ggtctgcgcg tcgtggagat atgtcggcat
     6421 cggggctcgc tctcgcgttc atgaagccag cccctatacc atcaccggcg tccgctcgct
     6481 gccgacatca cacagacgca cgacccttcg tatcaaaggc ctatcactcc gccgcatcca
     6541 tcatcggggg agccgcggcg ctcgcgtcat cgagcatgcc gagcgcgtgc aggtaggtat
     6601 cgagaacagc ctcctcctcg agccggtccg tcttgctctt cttgcggagc cggatcacct
     6661 ggcggaggac cttcacgtcg aagccgagcg ccttggcctc ggcgaacttg tcgcggacat
     6721 cgccggcgat gttcttcttc tcttcctcga gccgctcgat ctgctcgacg agctggcgca
     6781 gctgcccctg cgcggaagcc tgcaatgacg acatgtcacc tcaccccact ttgaacgctt
     6841 cggaccatcg cggcgttcgg ccgccagcat cggacgctag ggggccatct ccccggcatc
     6901 aaggcgtgca gcgccctcat ccaaagcttt ctcagcgtcc ctcggcgagt gcggctctcg
     6961 gtgtgcccga aacgctcaag cggcgccctt ggccgacagc gctggcagcc gcttgatggc
     7021 ggagatcgtg ctcccggccc tggcgatgcg ggtgacggcg tccgtcagcg gctcggatac
     7081 cgcgctcatg tgatgcgcgt tggcgtgcag cagcatcatg ccgtcggtca tcaccgcaac
     7141 atggccgggc cagaacacga gatcgccgcg ctttaggccg tcgagatcgg ccgggatgag
     7201 tacattctcg ccgagtgccg cctgctgcat gtcgctgtcg cgcggcgcct tcagccccgc
     7261 cgcctcgagt gcgagctgca cgagacctga acagtcgacg ccaatcnnnn nnnnnngtac
     7321 gcccgcccca caggtagggt gtcccggcaa accgctcggc gatgtcgaca aagtcggatg
     7381 catgacgatg gctttcggcg acgtgccgcg cgatcacgta tccgccggac gcgagcgcca
     7441 ggaacttctc gcccgcctcc gaaaccgaga gttccgagtt gagcgagaga tgcatgagcg
     7501 gcggcgactt gatgtccggc tcggaataga gaaatgtgcc gagcgccttc acacggtgcc
     7561 ccgtctcgcg cgtgctgcgc gtcagagcat cggccggcac gtaacccaca tagcggtccc
     7621 gcgccagctg cacccaggcc caacccccct gctcctcgaa gacggtcacg cgctcgccga
     7681 acagggcctc cgtcagcagc ccctcgcctg aggttggtgc cgcgcgaacc gcgaccgagc
     7741 cgcggaccac ctgcgcctcg gcgccggtgg tgaaccgctc cgcctcgacc attccggcaa
     7801 gctccgccgc cgcaaggtct gcccgatagg cgttgacgcg cctgtcgaga cgatgtgagc
     7861 cacctgccgc catgccgacc ccgcgcgccg ctgcggcgcc tgttgccgct cctggctgat
     7921 gcttacgccg aaagcaactc agacggtgcg cccgtgtgtg cgcttcagcc attcgaacac
     7981 ggcgcgcgca ccctgcggct cgccgccctt cgggccgatc ggtttggctg ccggccgcca
     8041 accgtagatg tcgaaatggg cgaagcgctt cgcacgtttg acgaaccgct tcagaaacat
     8101 ggcgccgacg atcgagcctg caaacccgcc ctccgctaca ttattcatgt cggcaacagg
     8161 gctgtcgagc cagctctcgt agggcgtcgt catgggcagt cgccacatcg gatcgccgac
     8221 ggcctctcct gctgccgcca gcgccgtggc cgtcgcgtcg tcatcgcaga agaccggtgg
     8281 caggtcgggc ccgagggcga cgcgggcagc gccagtcaga gtggcgaacg tcatgagcac
     8341 gtcgggcgta tcctcgtcgg cgagcgccag cgcgtcggcg agcacgagcc ggccttccgc
     8401 atccgtattg ccgatctcga ccgtgcggcc gccacggctc ttgagcacat cgccgggacg
     8461 gaacgcattg ccggcgacgg agttctcggc ggcggcaatg acgatgcgca gacgtacatc
     8521 cagccgtcgc gccatgatca tatgagcaag cgccagcacg gacgcggccc cacccatgtc
     8581 cttcttcatg agcagcatgc tggccgacgg cttgatgtcg aggccgccgg tgtcgaagca
     8641 gatgcccttc ccgacgagcg tgacacgcgg cgcgtcggcc cgcccccagg tgagctcgat
     8701 cagccgcggc gcgcgcggac tagcccggcc gaccgtgtga atcagtggaa aattgcgcgc
     8761 gagaaggtcg tcgccgatga tgacggaggc ttgggcatcg tggcggcggg ccaggtcgcg
     8821 tgccgccgct tcgagctccg ccggacccat gtcattggcc ggcgtattga tgagatcgcg
     8881 cccgatccac acggcctcta tcgcgctcag cacgttctcg cggttgacgc cgcgcggcgt
     8941 cttgagcaca gccagcgggt ctgcctcagc cgacttgtag cggcggaagc gataagcccc
     9001 gaggccatag gccgtcgcgg caagctcggc atcggcaccg aggtcggtct caagatggta
     9061 gccgccaggc ggcagcagcg tcggcagcag gccggcgagc agcgcgatcg gctcgccctt
     9121 ccccgcctgc ggcgggccga tgccgagcac tgccccggcg atgccgcctt caggcgatgg
     9181 cagcagcagg aagcgccgcg ccgtcccctt gaagccatgc gacttgagcc acgccctctg
     9241 ctcgccgctc aaagccggaa gcacgtcgcc ctcccggcct tcccgtacga gccagatggg
     9301 gcgggtctgg cgcttcgtgg cgccatcgat gatgatatcg gccgggctct gcggccaaga
     9361 cttctctcgt gtcgtcatga gctcgatgct acacgggttg agccggggtc aaccggcggt
     9421 cgcaggcagt cgttaacatt caccatgggt gccgacggca caaccgcggc gatcggtcca
     9481 aacggagata tcaacggtca tggtggctca gcagtatcgg cttgtgatcg gctccaagaa
     9541 ctggtcgtcc tggagtctca ggccctggat cgccctcaag cgcttctcga tccccttcga
     9601 tgagaccaac atcgatctga gggccgccga ccgcagccag cagatcgcgg cgagttcacc
     9661 ctccgccaag gtgccggtgc tgctcatcga tggcgagccg gtctgggaga gcctcgccat
     9721 tctggagacg ctcgccgacc gccacccaga gcatgccttc tggccgaaag cggcaccagc
     9781 ccgtgctatg gcgcgtgccg tcgccaacga gatgcacgcg ggctttgccg atctccgcca
     9841 gcattgcccg atggatttcc tcggccgcgc ccccatggcg agcctcccgg accccgtcgc
     9901 cgccaacgtc cgccgcatcg tcaaggtctg gggcgaggcc cgcgtcaggc atggcgccgg
     9961 cggaccgttc ctgttcggcg cgttctcgat cgcggatgcc atgtatgccc cggtcgtc
//
LOCUS       DAORNH010000164         9932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5038_length_9932_cov_2.794877, whole genome shotgun sequence.
ACCESSION   DAORNH010000164 DAORNH010000000
VERSION     DAORNH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5038_length_9932_cov_2.794877"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PK264_15730"
     CDS             <1..204
                     /locus_tag="PK264_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HRD77357.1"
                     /translation="HILSERNPVTEISSIRLGKRAGRARIASNVRLAESQRVVALARL
                     SDGSFLRGETSVLLTLAACIDGG"
     gene            217..537
                     /gene="soxZ"
                     /locus_tag="PK264_15735"
     CDS             217..537
                     /gene="soxZ"
                     /locus_tag="PK264_15735"
                     /inference="COORDINATES: protein motif:HMM:NF020350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRD77358.1"
                     /translation="MAQTRIVLPSSARRGETIEIKTLIQHPMEPGYRRDAMGRAIPKH
                     VISSLVVTYAGEEIIRAALTQGIAANPYLSFWTTAVETGDLVFTWTDDRGGVTSETRT
                     LSVT"
     gene            705..1328
                     /gene="soxA"
                     /locus_tag="PK264_15740"
     CDS             705..1328
                     /gene="soxA"
                     /locus_tag="PK264_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HRD77359.1"
                     /translation="MLWVERGEALWKASASPGGASCQSCHGDPSLQMTGVSARYPRID
                     PGTGELRSIELQINACRTRRLSVAPFDWEAEALLAITALLAHRSRGAAISVATTDSAH
                     AHYERGRRLFHERQGQLNLSCANCHDENIGKNLRGDVISEGHPTGFPIYRLEWQTLGS
                     LQRRLRACSLGVRAEVLDFGSDDYVALELYLAARANGMRLEGPAIRK"
     gene            1477..4788
                     /locus_tag="PK264_15745"
     CDS             1477..4788
                     /locus_tag="PK264_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77360.1"
                     /translation="MFRLTRYFSIISALMIGAITVLFVYLYQRNEHNVLLASKHLRNV
                     ERGEAYSVELMKLFGDALLAAVGGNPKPLSSDNVAAIDTRVRRLALGSSVKSIRLHQP
                     SGASIYSSDHDRQPRGVSAGVKRVSSRRTAESNIAYRSTIATLSGPEQSLYVVQSLVP
                     YYLADRSVGFVLVIESDITRDVKAIHASTLHTAFLSVLALGFLYIALLLVVRRAERIL
                     GAQYRQLSTFGERLDAKVKERTQDLVDQQLLLSRLMRDDRIRQGTLRDAMATITAAVS
                     KQLGVQRVGIWIFEPEKACVTCVDLFDGKEETHSAGRNAAVQAMPRYFAALRQKQLIV
                     ADDAIADPQTSEIADSYLRPNGISSLLDAPILLDSKLGGIVSFEHKGPPRSWSPEEKL
                     IVVAVANLAAIVLERIERARITDELRKATVAAAAATKAKTEFLANMSHEIRTPMNGVF
                     GMTELLLRTELGDRQRRLVETVQQSASSLLKIINDILDISRIEARKLELDIQDVDIRS
                     CLESTVAIFVEPSHRQGIELGLHIASGVPQMIKGDPVRLRQIFVNLIGNAIKFTRRGE
                     VAVRVAVAEETAARTVLTFIVRDTGIGIEPSILDRLFEPFSQADTTISRRFGGTGLGL
                     SISKQLIEMMGGTIAIESVPNEGTSISIRLPVEVSATSSAARRKKLPDLRGRRILVVD
                     DRAMNREIVASYLADANVDVTSVDSGEKAMQGLRAATSEGRPFDAAILDVVMPDMNGL
                     DLAGAIKADRAIKPLKLSLLTSLSWKGDTWMARDAGVEAFLTKPIRRDELVEAIASLF
                     DGAARQTVPAVEPVTSAASHSSLRGHVLVVEDNPVNVEVARELITCLGCTVDVAWDGV
                     EGARAAAMKAYDAILMDVNMPNLDGFGATRQIRTQELKSKAKRVPIIGLTANAYSEDR
                     ERCLAADMDDYLSKPFTEQQIEAVLVRWLGKAAATADEPASGTTPDGPPAFSGATADV
                     PQRDERQVVDLKVLDKLHAIRPDMAERVTRLFLEHATRTLQEIDEVVAAGDVAALGAK
                     AHSLKSSSANVGAAELAALFARLEIEAREKQAAKCPATVAEIRQEFDRVVGALGSYDP
                     TRSSATETPASRRVA"
     gene            complement(4795..5736)
                     /locus_tag="PK264_15750"
     CDS             complement(4795..5736)
                     /locus_tag="PK264_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD77361.1"
                     /translation="MELRNLGHSGLRVSAIGLGCNNFGARIDDAAAKAVIHKAMDLGI
                     TLFDTADIYGNKGGSETTMGGILGARRKDIVLASKFGMQMDEAGVMKGGARRYIMSAV
                     EASLKRLKTDWLDLYQLHQPDPLTPIEETLRTLDDLVRSGKVRYIGCSNLAGWQVADA
                     QWTSRHNGLASFISAQDEYSLLFRDHEKQLFPALHHYGMGLLPYFPLACGMLTGKYER
                     GKAMPAGSRLSTMKNRADQYMTDTNWAIVDKLAAFAKARGHTVLDLAMSWLAQRPPVT
                     SVIAGATSAAQVEANVKAASWTLSADDMAEVARLASK"
     gene            5812..6861
                     /locus_tag="PK264_15755"
     CDS             5812..6861
                     /locus_tag="PK264_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRD77362.1"
                     /translation="MPKVAAERLRQIARTLLEAAGASKEEAEVVARLNIAANLAGHDS
                     HGIILIPTYIDRVRKGHIVPKAPFTVLKETATTTVIDGNWGFGYAVTERATKLTIEKA
                     RTGNVAATTVLRQSHIGRVGAYPIMAAKEGFIAIMTADSGRSPKHVAPFGGTKARLGT
                     NPIAIAMPSDLAAPFCLDMATSSVAVGKIKLAEARGQKIPEGWVVDKDGQATTDPAAY
                     GKGGVLLPLGGSEGYKGYGLSAMVEIFAGILPGLGFGVEPSGRHNDGCFLALFKVEAF
                     RPLDVFKREVREFAEYLKSTPPQNGVKEVLYPGEIEWRTEEAREKAGVEIEDATWSKL
                     VALGKELGVGDQVKA"
     gene            complement(6883..>7172)
                     /locus_tag="PK264_15760"
     CDS             complement(6883..>7172)
                     /locus_tag="PK264_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77363.1"
                     /translation="VNSRGKRWPVPEMRRAALAWGMALIALTLVACTVHFWNVLHAYF
                     FFFLGLAGWLADPWRHAAGRSADPAAKARADVGRPRRVGYWADQPEPALAQ"
     assembly_gap    7173..7193
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7194..8227)
                     /locus_tag="PK264_15765"
     CDS             complement(<7194..8227)
                     /locus_tag="PK264_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77364.1"
                     /translation="MRRLVCDAPWPVVLLVASFLAPTEFSLYLGGMRLPPHRVALLLL
                     ILPACWRMARGRDGLKMRIFDVLFAAYAAWTVGVFMHHQGQAEGLQFGGSLALESFAA
                     YMVARAWIRDSTVYAATIRLLIGAIAVSGLIALPEAVLGIHYVHDILRELTGYHHPIG
                     HETRLGLTRAYGTFDHPIHLGTFCASIVALVWFSEPRLNRARKKVAVVTAATFMGLSS
                     APLLCLGLQGSFVGWDKLTRGIKGRFALTLFAVAILYAGLSLVMTRSPFTIIATGMVL
                     DSWTGYYRMVIWEWGLINVWANPLTGIGLGDWERPWWMISASVDAFWLVVTMRAGIPA
                     FLLLVAAIVAL"
     gene            complement(8254..>9476)
                     /locus_tag="PK264_15770"
     CDS             complement(8254..>9476)
                     /locus_tag="PK264_15770"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HRD77365.1"
                     /translation="NSGMYDTSRMHDLPLSPGRILAALAISFLAVLGLGLPFAPREVH
                     LWVWYGAWMSTSYALLLNLRIIARWLLARLTAAGTFDMRVAVYGGGVIASRVDQQLRN
                     PALGIKFVGVYDDRPEQRLDASSPTLAGRLDDLVAAARSGRIDQIVIALPQGADQRTA
                     TIARMLERLPVSLHVVTHIASDLVDETSVHRVSSIGSVGLIDVKGKPLADWSGIVKAV
                     EDYVLGALFLLASLPLLAIVALAVKLDSPGPVLFRQKRVGRNGRIFDCLKFRTMTVLE
                     DGPDLQQVRKDDPRVTRVGKWLRMTSLDELPQLINVLRGEMSIVGPRPHALAHDERFA
                     EVLARYANRGQVKPGITGLAQVEGLRGETETLDKLEARLAKDLAYVNNWSLWLDMKII
                     ARTFALGFLGRNAY"
     assembly_gap    9477..9520
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9521..9719)
                     /locus_tag="PK264_15775"
     CDS             complement(<9521..9719)
                     /locus_tag="PK264_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77366.1"
                     /translation="MAANTLTAGQSVAPASERPRRSRRIALDLVGFADALAVAAGGLV
                     PAFIYAKIAGLPINWAAQFQAG"
BASE COUNT         1798 a         3205 c         3287 g         1577 t
ORIGIN      
        1 cacattctct cggagcgcaa tcccgtcacg gagatttcct cgattcggct cggcaagcgg
       61 gcggggcggg cgcgcatcgc gagcaatgtc cgcctcgccg aatcgcaacg cgtggtcgca
      121 ctggcgcggc tcagtgacgg ctcgttcctg agaggcgaga ccagcgtctt gctgacgctc
      181 gccgcttgca tcgacggcgg ctgacggagg tacaccatgg cccagacccg gatcgtcctc
      241 ccgagcagcg cacggcgcgg cgagactatc gagatcaaga cgctcatcca gcacccgatg
      301 gagccaggct accggcgcga cgccatgggc cgcgccatcc cgaagcatgt catctcgagc
      361 ctcgtcgtca cctatgcggg cgaggagatc atccgcgccg cactcaccca aggcatcgcg
      421 gccaatccct atctctcgtt ctggacgacg gcggtagaaa cgggcgacct cgtgttcacg
      481 tggacggacg atcgcggcgg cgtcacctcc gagacacgca cactcagcgt cacatgaggc
      541 gaccgttcgc cggccgcggt tcagttgcgc ttgcagcact ccttctcctc gcttctctgg
      601 ccactgccga aacgccagcc ccagacgacc ggcggaaaac cggaacgtcc tacctttcgc
      661 ccgagcttcg gcgccggtct ggcgacgacg gcgccaaccc cgccatgctc tgggtcgagc
      721 ggggcgaggc cctgtggaag gcatcggcat cgccgggcgg cgcgtcgtgc cagagctgcc
      781 acggcgaccc gagcctgcag atgacgggcg tcagcgcacg ctatccgcgc atcgaccccg
      841 ggaccggcga actccgctcg atcgagttgc agatcaacgc ttgccggacg cgccgtctat
      901 ccgttgcccc gttcgactgg gaggcggaag ctctgctggc catcacggcc cttctcgcgc
      961 atcggtcccg cggcgctgcc atttccgtcg caacgacaga ctcagcgcac gcacattacg
     1021 agcgcggcag gaggcttttc catgagcggc agggccagct caatctttca tgcgccaact
     1081 gccacgatga gaatatcgga aagaatctcc gcggtgacgt gatcagcgaa ggccacccga
     1141 cagggtttcc catctatcgc ctggaatggc agacactcgg ctcgctgcag cgccgcctgc
     1201 gggcctgcag cctgggtgta cgcgcggaag tgctcgactt cggttcggac gattacgtcg
     1261 cgctcgaact ctatctcgcg gcccgcgcca acggcatgcg cctggaaggc cccgcaatcc
     1321 gtaaatgaat tgatgccgga tgtgccttgc gaacacaaaa tcccgcggcg gaatgtgatt
     1381 tctggctcct gtagggtgca aatacgtcgt ccacgtccag accctgttag gcaatcacac
     1441 ttaagctctc ctctaagtag agtgccgagg ggaccgatgt tcaggctgac ccgctacttt
     1501 tcgatcatca gcgcgctcat gatcggggcg atcacggtcc tgttcgtcta tctgtatcag
     1561 cgcaacgagc acaacgtcct tctggcctcg aagcacctcc gcaacgtcga gcgcggcgag
     1621 gcctattcgg tcgagctgat gaaacttttc ggcgacgcgc tgctggccgc agtcggcggc
     1681 aacccgaaac cgctttcatc cgacaatgtc gccgcgatcg atactcgcgt gcgccgcctc
     1741 gcactcggct cgagcgtgaa gagcattcga cttcaccagc cgagcggagc atcgatttat
     1801 tcatcggacc atgaccggca gccgcgcggc gtatcggcgg gcgtgaagcg cgtctcaagc
     1861 cgccgaacgg ctgaaagcaa tatcgcgtat cggagcacga tcgccacgct gtccggacct
     1921 gagcagtcgc tctatgtggt ccagagtctg gtgccctatt acctggccga tcggtcggtc
     1981 ggtttcgtgc tggtgatcga gtccgacatc acgcgcgatg ttaaggcaat tcatgcgagc
     2041 acgctgcaca cggcatttct gtcggtgctg gcgctcggat ttctctacat cgccctattg
     2101 ctcgtcgtcc ggcgcgccga gcggatcctt ggcgcgcagt atcgccagct ttcgacattc
     2161 ggcgaacggc tcgatgccaa agtgaaggaa cgcacgcagg acctcgtcga ccagcagcta
     2221 ctgctgagcc gcctgatgcg ggacgaccgt atcaggcagg gcacactccg cgacgcaatg
     2281 gcgacgatca ccgccgcggt atcgaagcag ctcggcgtcc agcgcgtggg catctggatc
     2341 ttcgagcctg aaaaggcctg tgtcacctgc gtcgacctct tcgatggcaa ggaggagaca
     2401 cattcggcag gccggaatgc ggcagtgcag gccatgccgc gctacttcgc ggcgctccgc
     2461 cagaagcagc tgatcgtcgc cgatgacgcg atcgccgatc cgcaaacctc ggaaatcgcc
     2521 gacagttatc tgaggcccaa cggtatctcc tcgctcctcg acgcgccgat cctgctcgac
     2581 agcaagcttg gcggcattgt gagcttcgag cataagggcc cgccgcggtc gtggtcgccg
     2641 gaggaaaagc tcatcgtcgt tgccgtggca aacctcgcgg cgatcgtgct cgagcggatc
     2701 gaacgggcac gcatcaccga cgagctgcgc aaggcaaccg tcgccgccgc ggccgcaacg
     2761 aaagcgaaga ccgagtttct cgccaatatg agccacgaga tccgaacacc catgaacggc
     2821 gtgttcggca tgacggaatt gctgctgcgc accgagctcg gcgatcgcca gcgccgcctc
     2881 gtcgagacgg tccagcagtc ggcgagcagc ctgctcaaga tcatcaacga catcctcgac
     2941 atctcccgca tcgaggcgcg caagctcgag ctcgacatcc aggacgtcga tattcgctcg
     3001 tgcctcgaga gcacagtcgc aatcttcgtc gagccgtcgc accgccaggg catcgagctc
     3061 ggcctgcaca tcgcatccgg cgtgcctcaa atgatcaagg gcgacccggt gcgcctgcga
     3121 cagatcttcg tcaacctgat cggcaacgcc atcaagttca cgcgtcgcgg cgaggtcgcc
     3181 gtgcgcgtcg cggtcgccga ggagacagcg gcccggacag ttctcacctt catcgtccgc
     3241 gacacgggca tcggcatcga gccatcgatc ctcgaccgcc tgttcgaacc gttctcgcag
     3301 gcggatacca cgatcagccg ccgcttcgga ggcacgggcc tcggcctctc gatctcgaag
     3361 cagctgatcg agatgatggg tggcaccatt gccatcgaga gcgtgcccaa cgagggcacg
     3421 tcgatctcca taaggctccc ggtcgaggtg tcggcgacga gctctgcggc tcgccgcaag
     3481 aagctgcccg accttcgcgg ccgccgcatt ctcgtcgtcg acgatcgggc catgaaccgc
     3541 gagatcgtcg cgtcctacct tgctgatgcc aacgtcgacg tcacatccgt cgacagcggc
     3601 gagaaggcga tgcagggcct gcgcgcagca acgtccgaag gcaggccatt cgatgcagcc
     3661 atcctcgacg tcgtgatgcc ggacatgaac gggctggacc tggccggtgc catcaaggcc
     3721 gatcgggcca tcaagccctt gaagctctcg ctgctcacct cgctcagctg gaagggcgac
     3781 acctggatgg cgcgggatgc cggtgtcgag gcgttcctga cgaagccgat ccggcgcgac
     3841 gaactcgtcg aagccatcgc atcactgttc gacggcgcgg ctcgccagac ggtgccggcg
     3901 gtcgagcctg taacctctgc cgcgtcgcat tcgagcttgc gggggcacgt cctcgtggtc
     3961 gaggacaatc cggtcaacgt tgaggtcgcg cgcgaactga tcacctgtct cggctgcacg
     4021 gtggacgttg cctgggacgg cgtcgaaggc gccagggccg cggcgatgaa ggcctacgat
     4081 gccattctga tggacgtgaa catgccgaac cttgatggct tcggtgccac ccggcagatc
     4141 cgcacgcaag agctgaaatc caaggcgaag cgcgtgccga tcatcgggct caccgcaaac
     4201 gcctattccg aggatcgcga gcgctgcctt gccgcggaca tggacgacta cctgagcaag
     4261 ccgttcacgg agcagcagat cgaagccgtc ctggtgcgct ggctcggcaa ggcggcagcg
     4321 actgccgacg agccggcgag tggaacgacg cctgatgggc cgcctgcctt ttcgggcgca
     4381 acggcggatg tgccgcagcg tgacgagcgg caggtcgtcg atctcaaggt cctcgacaag
     4441 ctccatgcaa tcaggccgga catggcggag cgtgtcacgc gacttttcct cgagcacgca
     4501 acgcggacgc ttcaagagat cgacgaggtt gtcgcggcgg gggacgtcgc agcgcttggc
     4561 gcgaaggccc acagcctcaa atcatcgagt gccaatgtcg gcgccgcgga gctcgcagcc
     4621 ttgtttgccc gcctcgagat cgaggcccgc gagaagcaag cggccaagtg ccccgcgact
     4681 gtcgcggaga tcaggcagga attcgatcgc gtggtgggcg cgctcggctc ctacgacccg
     4741 acccgatcgt ccgcaactga gacgccggcg tcgcgccgcg tcgcctgagc ctagttactt
     4801 tgaagcgagc cgcgccactt cggccatgtc gtctgccgaa agcgtccagc tcgccgcctt
     4861 cacgttggcc tcgacctgcg ccgcactcgt ggcgccggcg atcacgctcg tcaccggcgg
     4921 gcgctgagcg agccagctca tggccagatc gaggacggtg tgcccgcgtg ccttggcgaa
     4981 ggcggccagc ttgtcgacga tcgcccagtt tgtatccgtc atatactggt cggcgcggtt
     5041 cttcatggtc gagaggcgcg agcccgccgg catcgccttg ccgcgctcgt acttgccggt
     5101 gagcatgccg caggcgagcg ggaagtaggg gaggagcccc atcccgtagt gatggagcgc
     5161 ggggaagagc tgcttctcgt gatcgcggaa aagcagactg tactcgtcct gcgccgagat
     5221 gaagctagcg agcccattgt ggcgcgatgt ccattgcgcg tccgcaacct gccaaccagc
     5281 gaggttcgag cagccgatgt agcgcacctt gcccgagcgg acgagatcgt cgagcgtgcg
     5341 cagcgtctcc tcgatgggtg tcagcgggtc gggctggtgg agctggtaga ggtccagcca
     5401 gtcggtcttg agacgcttga ggctcgcttc gacggcactc atgatgtagc gccgcgcccc
     5461 gcccttcatg acgcccgcct cgtccatctg catgccgaac ttgctggcga gcacgatatc
     5521 cttgcgcctg gcgcccagga tgccgcccat ggtcgtctcg gagccgccct tgttgccgta
     5581 gatgtcggcc gtatcgaaga gcgtgatgcc gaggtccatc gccttgtgga tgacggcctt
     5641 ggcggccgca tcgtcgatgc gcgcgccgaa gttgttgcag ccgaggccga tcgctgagac
     5701 gcgaagtccc gagtggccga gattgcgcag ttccatgggc tcgctccttg cgcggtgcgc
     5761 ggatcaggat aggttcgagc caagtatagg cgccggaccc tggaggatgt catgcccaaa
     5821 gttgccgccg agagacttcg ccagatcgcg cgcacgctgc tcgaggcggc cggcgcctcg
     5881 aaggaggaag cggaggtcgt cgcgcgcctg aacatcgccg ccaatctcgc ggggcatgat
     5941 tcacatggca tcatcctgat cccgacctac atcgaccggg tgaggaaggg gcatatcgta
     6001 cccaaggcgc ccttcacggt tctcaaggag acggcgacga caaccgtgat cgacggcaac
     6061 tggggcttcg gctatgcggt gaccgagcgg gcgaccaaac tcacgatcga gaaggcgcgg
     6121 acgggcaacg tcgcggcgac gaccgtgctc cgccagagcc acatcgggcg cgtcggcgcc
     6181 tacccgatca tggcggcgaa ggagggcttc atcgccatca tgacggcgga cagcggtcgg
     6241 agccctaagc acgtcgcccc attcggcggc accaaggcgc ggctcggcac caatccgatt
     6301 gccatcgcca tgccgtcgga cctcgcggca ccattctgcc tcgacatggc gacgtcgtcc
     6361 gttgcggtcg gcaagatcaa gctcgcggag gctcgcggac agaagatccc cgagggctgg
     6421 gtcgtcgaca aggacggcca agcaaccacc gatcctgcgg cctacggcaa gggcggcgtg
     6481 ctgctgccgc tcggcggcag cgagggctac aagggctacg gcctctcggc gatggtcgag
     6541 atcttcgccg gaatcctacc gggcctgggc ttcggcgtcg agccctcggg ccgtcacaac
     6601 gacggctgct ttctcgctct cttcaaggtc gaggcgttcc gcccgctcga cgtcttcaag
     6661 cgcgaggtgc gcgagttcgc ggagtacctg aagtcgacgc cgccgcagaa tggcgtgaag
     6721 gaggtgctct accccggcga gatcgagtgg cggaccgaag aggcgaggga gaaggccggg
     6781 gtcgagatcg aggacgccac ctggtcgaag cttgtcgcgc tcggcaagga gctcggcgtc
     6841 ggcgaccagg tcaaggcttg aggcctgcag cgccgtacgc ggctactgcg cgagcgcagg
     6901 ctccggctga tcggcccagt agccgacgcg acgcgggcgt ccgacgtcgg cgcgcgcctt
     6961 ggccgctgga tcggcagagc gaccggcggc atgccgccag ggatcggcga gccagcccgc
     7021 gagcccgagg aagaagaaga agtaggcgtg cagcacgttc cagaagtgca ccgtgcaggc
     7081 aacgagggtc agcgcgatca gcgccatgcc ccaggccagg gccgcccggc gcatctcggg
     7141 caccggccag cgcttgccgc ggctgttcac cgnnnnnnnn nnnnnnnnnn nnnagcgcaa
     7201 cgatggccgc gacgagcagc aggaacgcgg gaatgccggc gcgcatcgtg acgaccagcc
     7261 agaaggcgtc gaccgaggcc gagatcatcc accacggccg ttcccagtcg ccgaggccga
     7321 tgccggtcaa cgggttggcc cagacattga tgaggcccca ctcccagatc accatgcggt
     7381 agtagccggt ccagctatcg agcaccattc cggtcgcaat gatcgtgaag ggcgagcggg
     7441 tcatgacgag cgagaggccg gcgtagagga tcgcgaccgc aaagagcgtc agcgcgaagc
     7501 ggcccttgat gccgcgtgtg agcttgtccc agccgacgaa gctcccttgc agaccgaggc
     7561 agaggagcgg cgccgaggag agccccatga aggttgccgc ggtgacgacg gccaccttct
     7621 tgcgcgcgcg attgagccgc ggctcggaga accagacgag cgcgacgatg ctcgcgcaga
     7681 atgtaccgag atggattggg tgatcaaagg tgccataggc gcgggtgaga ccgagccgtg
     7741 tctcgtggcc gatcgggtgg tggtagcccg tgagctcgcg caggatatcg tggacgtagt
     7801 ggatgccgag cacggcctcg ggaagcgcga tcagtccgga tacggcaatc gcgccgatca
     7861 gcaggcgaat ggtcgcggca tagacggtgc tgtcgcgtat ccaggcgcgt gccaccatgt
     7921 aggcagcaaa gctctcgagc gcgagggagc cgccgaactg cagcccctcg gcctgcccct
     7981 gatggtgcat gaacacgccg accgtccagg ccgcgtaggc cgcgaacagg acgtcgaaga
     8041 ttcgcatctt gaggccgtca cgcccgcgcg ccatgcgcca gcaggcgggc aggatgagga
     8101 ggagcagcgc cacgcgatgc ggcggcagcc gcatgccgcc gaggtagagc gagaactcgg
     8161 tcggcgcgag aaagctcgca acgagcagca cgaccggcca gggggcatca cagacgagcc
     8221 ggcgcatggc gtgtgctctc cgcaagccgc agctcagtag gcgttgcgcc cgaggaaacc
     8281 cagcgcaaat gtccgggcga tgatcttcat gtcgagccag agcgaccagt tgttgacgta
     8341 ggcgagatcc ttcgcaagtc gcgcctcgag cttgtcgagc gtctcggtct cgcccctgag
     8401 gccctcgacc tgcgcgagcc ccgttatccc gggcttcacc tggccccgat tggcatatcg
     8461 ggcgagtact tcggcaaagc gctcgtcgtg ggccagcgcg tgcggacgcg ggcccacgat
     8521 cgacatctcg ccgcggagca cattgatcag ctgcggcagc tcatcgaggc tcgtcatgcg
     8581 cagccacttg ccgacgcggg tgacgcgcgg atcgtccttg cgcacctgct ggagatcggg
     8641 cccatcctcg aggacggtca tggtacggaa tttgaggcag tcgaagattc ggccgttgcg
     8701 cccgacgcgc ttctgacgga agagcacggg gccggggctg tcgagcttga cggcgagtgc
     8761 cacgatcgcg agcagaggca gcgaggcgag caggaagagc gccccgagca catagtcctc
     8821 gaccgccttc acgatcccgc tccagtcggc gaggggcttg cccttcacat cgatgagccc
     8881 gacgctgccg atgctcgaca cgcggtgcac cgacgtctcg tcgacaagat cggaggcgat
     8941 atgcgtcacg acgtggagcg agaccggaag acgctcgagc atacgagcga tcgttgccgt
     9001 gcgctggtcg gcaccctgag gaagggcgat gacgatctgg tcgatccgtc cggagcgggc
     9061 cgcagcgacg aggtcgtcga gccggccggc cagcgttggg ctcgacgcgt cgaggcgctg
     9121 ctcgggccgg tcatcgtaga cgccgacgaa cttgatgccg agggccgggt ttcgcaattg
     9181 ctggtcgacg cggctcgcga tgacgccgcc accgtagacg gcgacacgca tatcgaatgt
     9241 gccggcggcg gtcagccgag cgagcagcca gcgcgcgatg atgcgaaggt tcaggagcag
     9301 cgcgtagctc gtggacatcc aggcgccata ccagacccac agatgcacct cgcgcggggc
     9361 gaacggcagc ccgaggccga ggacggcgag gaacgaaatg gcgagtgctg caaggatgcg
     9421 gccggggctc agcggcagat cgtgcatgcg cgaagtatcg tacatgcccg agttggnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcccgcctg gaactgtgcg
     9541 gcccaattga tcggcaggcc cgcgatcttc gcgtagatga aggccggaac gagaccgccc
     9601 gcggcaacgg ccagtgcatc ggcgaagccg acgaggtcga gtgctattcg ccgcgatcgg
     9661 cgtggacgct ccgaggccgg cgccacagat tgacctgcgg tgagcgtgtt cgctgccatt
     9721 gtgtcctcat ccgacgcgcg acaccggcgt gaggcgccgg tcatatcggc gcatttgacg
     9781 cgactttggt gaagattgcc tgaaagagaa cgcatgaagg cgccgccacg tcgggggaga
     9841 cgtgacggcg ccggcaatcg ctcggaggtg ggttcgcccg gggggcgagg gtcaggccgc
     9901 cacgtcctcg tcaccggggt cgcgcagcac at
//
LOCUS       DAORNH010000165         9835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5103_length_9835_cov_2.673620, whole genome shotgun sequence.
ACCESSION   DAORNH010000165 DAORNH010000000
VERSION     DAORNH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5103_length_9835_cov_2.673620"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>537
                     /locus_tag="PK264_15780"
     CDS             <1..>537
                     /locus_tag="PK264_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HRD77367.1"
                     /translation="MRGLGTPLTQTEIKDLGNQIASAASTTLSQKKPIVSVSILYRDR
                     PIRAQVIQPPAVEGGFSISLRFFSSLPLKAIKLGFLYGKERSLEGLRRERNAALRDVV
                     ASGDIDAALRFCVENKLNMIVSGGTSTGKTVAARKILSLIPPEERIITIEEAAELRPE
                     QPNAVTLIADRDTEARSA"
     assembly_gap    538..652
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<653..1044)
                     /locus_tag="PK264_15785"
     CDS             complement(<653..1044)
                     /locus_tag="PK264_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77368.1"
                     /translation="MLRSLRLALSVLALSGLAYSHFATTSGRAEEPAFAHPGVSADAT
                     ALEAVIKALAKTGTGQATGTKSSARPTRATAEALLKGGDAAGAVMAAGLDNHVDGALD
                     VVDHVGIGHRHIGLERGYGETADGQLRG"
     gene            1390..1689
                     /locus_tag="PK264_15790"
     CDS             1390..1689
                     /locus_tag="PK264_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77369.1"
                     /translation="MQRVIELAQRTIDGALLGAGYTLVAVSLAQLFAVTRRLNLAFGS
                     LALAGVALGIFVAHRAALGAGYRHIAGWAALVAALAPIPRGRVCEGALSDAGRPA"
     gene            1697..2584
                     /locus_tag="PK264_15795"
     CDS             1697..2584
                     /locus_tag="PK264_15795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRD77370.1"
                     /translation="MPALSPQSIALLSEFGIAALLALSFYLLLISGIVSLGQQALFGM
                     GAYAAGLSAVVWSWPLWAGLAAGVVAAAFTSLVPGLLARRIDGLRFTLATLGLAEIAR
                     HLIEHLVSPSMAGAAAAAAIKGVRLAPESLLALILALLMIVAAAITLVERSDTGRRLR
                     AMGEDATLAGVQGIDCGALRLAVNVASGAIAGLGGALQAFHAPLVRPDMLGLVAGVEG
                     IAHALVGGLGSLGGPLLAVAADVALLRATGLPVELRTVVLATVTVLVLLRRPRGLIDE
                     AFLRDLRAHIARGRTDARR"
     gene            2598..3278
                     /locus_tag="PK264_15800"
     CDS             2598..3278
                     /locus_tag="PK264_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77371.1"
                     /translation="MRGRVRALDGIDLLIRPREAVGLVGSRGAGKTTLLDCISGLVRP
                     ARGRIYMEERLLTRRAPFEIARLGVTRTFAVPRMFRRLSVIDNVRPFVPTGGSPRPAQ
                     MFARMMLGLVGLDERAADPIEALTPFELRRLELARALAPGPRLILLDEPARGLSGREA
                     RTMLGLIRRLALPHAAVLIAEVEPELTRELCPRTLVLEAGRKLRDGPAGPAMLAPPNE
                     TVQRVAEG"
     gene            complement(3306..>4064)
                     /locus_tag="PK264_15805"
     CDS             complement(3306..>4064)
                     /locus_tag="PK264_15805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD77372.1"
                     /translation="CTLLLIAAAAMGALVDVRRLLVPAVGLRIIGEIGASMLFNAALL
                     RMPIANAGAILQFIPLVTTAVAARLFGEKVGWQRWLMGAVGFVGVLFVLKPGAEGFNA
                     WSLLAVGSMLSMCLRDLATSRIPPTLPTLTIAALTAGCAGLAGCLLFPFETWIMPSPR
                     AAGFVVLAGVMMAFGFICLVVGMRSADMSLLAPFRYASVLWAILIGYLVWNEMIDGVA
                     AIGIALVVGAGLATLFRERTVVRRRIAGRSARGS"
     assembly_gap    4065..4115
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4208..5170)
                     /locus_tag="PK264_15810"
     CDS             complement(4208..5170)
                     /locus_tag="PK264_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD77373.1"
                     /translation="MSLASPGGPERLPLSANLKGIIAMVAAMASFSVGDTIMKLVSAT
                     DMPTSELIFVRGSVVLSAAIFIMIVWGGLEHIRQALSWAMALRAGGDVGGGWFFQSGL
                     ARMPLADLMSVIQLTPLSITAASAVFLGEKVGWRRWTATGVGLVGVLLIIRPGSSAFN
                     WWALAGIASVLCATVRDLATRQVDRRVPATMIMMLSALAVVLTSGAVAPFANWSRPDA
                     GSLALLACAAVCSLIGQLCVIIAMRTGEVSAVAPFRYAIIVFSLISSFLVWGYFPDRL
                     TLAGIAIVAAAGLYTFRREQHLARLRRMKELSESHGSPADRGAA"
     gene            complement(5167..>6109)
                     /gene="moaA"
                     /locus_tag="PK264_15815"
     CDS             complement(5167..>6109)
                     /gene="moaA"
                     /locus_tag="PK264_15815"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HRD77374.1"
                     /translation="TDRCDFRCVYCMSEHMTFLPKKDLLTLEELDRVCSAFVAAGVSK
                     LRITGGEPLVRKNIMQLFRSLSRHLESGTLEELTLTTNGSQLPKFAGELKAAGVERIN
                     ISLDTLRPDRFKAITRWGDFGGVMDGIVAAKRAGLAIKLNAVALKGVNEDEILDLVRY
                     AHGEGFDITFIETMPLGDIDGDRTEQYMPLSIVRARLMDHLTLSDVPYRTGGPARYVE
                     ARETGGRIGFITPMTHNFCESCNRVRLTCTGTLYMCLGQEDAADLRTPLRASPDDAAL
                     RAAINDAIRRKPKGHDFIIDRRTKRPSVGRHMSVTGG"
     assembly_gap    6110..6123
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7339..7509)
                     /locus_tag="PK264_15820"
                     /pseudo
     CDS             complement(7339..7509)
                     /locus_tag="PK264_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(7568..8683)
                     /locus_tag="PK264_15825"
     CDS             complement(7568..8683)
                     /locus_tag="PK264_15825"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HRD77375.1"
                     /translation="MPETHRAAAATSGILPAQAIRALIAARAIRMTEPASQGQVQPAS
                     LDLRLGPMAYRVRASFLPSPGGSLDTKLDALSLHAIDLTHGAVLETGCVYVVPLLEAL
                     ALPDDVSASANPKSSTGRLDVFTRVIADGVPSFDTIPVGFEGRLFAEICPQTFPIVAR
                     RGSMLSQIRFRRGDPVLDDGALARLHARHTLVTGGATADIRGGIALSVDLARRAEGPA
                     GYRAKRHTGIVDVDKPGACALSDYWEALPAPKDGRLVLDPDEFYILASKEAVHVPPDH
                     AAEMMPFNPLVGEFRVHYAGFMDPGFGHAAAGGAGARVVLEVRSHKVPFILDDGQIVG
                     RLVYERLLELPEILYGRDVGSNYQAQGLKLSKHFREG"
     gene            9547..>9835
                     /locus_tag="PK264_15830"
     CDS             9547..>9835
                     /locus_tag="PK264_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77376.1"
                     /translation="MFGLDELDDGLGGLDPCGLHEVRPESHRDWGATLTFALALAIRR
                     MRSDADRTAPLLWCATQASMREVGMPYAPGLLALGLSPDRLLMVETRRQDDI"
BASE COUNT         1618 a         3175 c         3250 g         1612 t
ORIGIN      
        1 catgcgcggc ctcggcacgc cgctgaccca gaccgagatc aaggacctcg gcaaccagat
       61 cgcctcggct gcgtcgacga cgctcagcca gaagaagccc atcgtctcgg tctcgatcct
      121 ctatcgcgac cggccgatcc gggcgcaggt catccagccg cctgctgtgg aagggggctt
      181 ttcgatttcg ctgcggttct tttcctcgct gccactgaag gccatcaagc ttggcttcct
      241 ctatggcaag gagcgcagcc tcgaaggcct gcgtcgcgaa cggaacgccg cgctccgcga
      301 tgtggtggcc tccggcgaca tcgatgccgc cctgcgcttc tgcgtcgaga acaagctcaa
      361 catgatcgtt tcaggcggca cctcgaccgg caagacagtc gcggcgcgca agatcctctc
      421 cctgatcccg cccgaagagc ggatcatcac gatcgaagag gctgcagaat tgcggcccga
      481 gcagccgaat gccgtgacgc tgattgctga tcgcgacacg gaggcccgca gcgccgannn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaccccg
      661 caactggccg tccgccgtct cgccatagcc gcgctcaaga ccgatgtgcc gatgacctat
      721 gccgacatgg tcgactacat cgagggctcc atcgacgtga ttatccaggc cggccgccat
      781 gacggcgccc gcggcatcac cacccttgag aagtgcctct gccgtcgccc tggtcgggcg
      841 ggccgatgat ttcgtgcccg tcgcctgccc cgtaccagtc ttggcgagcg ccttgatgac
      901 agcttcgagc gcggttgcat cggcactcac gcctggatgc gcgaatgccg gctcctctgc
      961 gcgcccggag gttgtggcaa agtgactgta tgccagtccg gagagtgcca gcaccgacag
     1021 agccaggcgc aacgatcgca acatgacgac ttcctgagtg gggatgaggc ttctgggttg
     1081 aactcggacc tgatcgcgct tgaggcgcac tctcggcggt gaagcctcgg ggcgcgggac
     1141 agtgcggctc aggcaatgga ggcgaggcgc aggacgtcct gcaatgaatt cgatcaagcg
     1201 agctgtgcat accgctttgg ccgattcatg gctgacggaa caattctaac tacgttccgg
     1261 catatgtcag ggaaggttcg agcggaatcc acggtcacga gcggcactcg gctgttgacc
     1321 gggcgacgaa gtcgtcgaac atgcgacggc aagccagtga cagggtcaga cggcgatcgg
     1381 atgagttttg tgcagcgtgt catcgagttg gctcagagga cgatcgacgg cgcgctgctc
     1441 ggcgcgggct acactctcgt cgccgtgtct cttgctcagc tttttgccgt gacgcgccgg
     1501 ttgaacctcg ccttcggcag cctggcgctt gcgggcgtgg cgctcggcat cttcgttgct
     1561 catagagccg cactcggcgc agggtatcgc catatcgccg gctgggcagc gctggttgcg
     1621 gcccttgccc ccataccgcg cggacgggtc tgcgagggtg cgctctccga cgccgggcgg
     1681 cctgcctgag ccgccgatgc ccgcactcag cccccagagc atcgccttgc tttcggagtt
     1741 cggcatcgcg gcgttgctgg cgctgtcatt ctatctgctg ctgatcagcg ggatcgtctc
     1801 gctcggccag caggccttgt tcggaatggg tgcttacgca gccggcctct ccgccgtcgt
     1861 gtggtcgtgg ccgctctggg ccgggctggc cgccggggtc gtcgcagccg cattcacgtc
     1921 gctcgtcccg ggtcttctgg cgcgtcgaat cgatgggctc cgctttacgc tggcgacgct
     1981 cggccttgcc gagatcgcac gccaccttat cgagcacctc gtgtcgccga gcatggccgg
     2041 tgctgctgcc gccgctgcga tcaagggcgt gcggctcgcg ccagagagcc tgctcgcgct
     2101 catcctcgca ttgctcatga tcgttgccgc agcgatcacg ctcgtcgagc gatccgatac
     2161 tgggcgacgg ctccgcgcga tgggcgagga cgcgacgctc gctggcgtgc agggcatcga
     2221 ctgcggcgcg ctgcggctcg ccgtgaacgt cgcctcgggt gcaatcgccg ggctcggggg
     2281 cgcgctgcag gcctttcatg cgccacttgt gcggcccgac atgctcggcc tcgtagcggg
     2341 tgtcgaaggc attgcgcatg cgctcgtcgg cggcctcggc tcgctcgggg ggccgctcct
     2401 cgccgtcgcc gctgacgtcg cgctcttgcg agcgacgggg ctcccggtcg aacttcgcac
     2461 ggtcgtgctc gccactgtca cggtgctcgt gctgttgcgc cgcccacgcg gcctgatcga
     2521 cgaggccttc ctccgcgatc ttcgcgcaca catcgcacga gggcggaccg atgctcgccg
     2581 ttgaggagtt gaccgtgatg cgcgggagag tgcgtgctct cgatggcatc gatctgctga
     2641 tccggccgcg cgaggccgtg gggctcgtcg gctcacgcgg cgccggcaag acgacgctcc
     2701 tcgactgcat cagtggactg gtgcgtcctg cccgcgggcg gatctacatg gaggagcggc
     2761 ttctcacgcg gcgcgcaccc ttcgagatcg cccggctcgg ggtcacgcgg acgttcgcgg
     2821 tgccgcgcat gttccggcgc ctgagcgtga tcgacaatgt ccggcccttc gtgccgaccg
     2881 gcggcagtcc gcgacctgct cagatgtttg cccggatgat gctcggcctg gtcggcctcg
     2941 atgagcgcgc cgccgatccg atcgaggcgc tgacgccttt cgagctccgc cgcctcgagc
     3001 ttgcccgggc gctggcgccg ggaccgcggc tgatcctgct cgacgagccg gcgagaggcc
     3061 tctccggcag ggaagcgcgg accatgctgg gtctcatccg ccgcctcgcg ctgcctcatg
     3121 ccgccgtcct cattgcggag gtcgagcccg agctcacgcg tgagctctgc ccgcgcacgc
     3181 tcgtcctcga ggctgggcgc aagcttcgag atgggccagc aggccccgcc atgctggcgc
     3241 cgccgaacga aaccgtgcag cgtgtcgcag aggggtgatc cgagccgcgg ccggcttggg
     3301 cgcggtcagg acccgcgggc agatcgccca gcaatgcggc ggcggacgac ggtgcgctcc
     3361 cggaacagtg tcgccagtcc ggcaccgacg acgagcgcga tgccgattgc tgcgactccg
     3421 tcgatcatct cgttccaaac caagtagccg atgaggatag cccacagcac ggacgcatag
     3481 cgaaacggcg cgagcaacga catgtcggcg ctgcgcatgc cgacgacgag gcagatgaag
     3541 ccgaaggcca tcatgacgcc ggccagcacg acgaagcccg cggcgcgcgg cgagggcatg
     3601 atccaggtct cgaatgggaa tagtaggcag cccgcgagcc cggcacaccc tgcggtcagc
     3661 gctgcaatcg tcagggtcgg cagggtcggg gggatccgac tcgtcgcgag gtcgcggagg
     3721 cacatcgata gcatcgaacc gaccgccagc agcgaccaag cgttgaaccc ctccgcaccg
     3781 ggcttcaaaa cgaagagcac gccgacgaag ccgacggcgc ccatcagcca gcgctgccag
     3841 ccgacctttt cgccgaagag gcgcgcggcg accgcagtcg ttacaagcgg aatgaactgc
     3901 aggatcgcgc ccgcattggc gatcggcatg cgcagcagtg ccgcattgaa gagcatcgag
     3961 gcaccgatct cgccgatgat ccggaggccg acggcgggca ccaggaggcg gcggacatcg
     4021 acgagcgcgc ccatggcggc ggccgcgatc agcaggagcg tgcannnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaggc aggcatcgct cgcgagcatg
     4141 ccgaagccgg cgccgatcac gcaggcgata ccgaggagcg ggcgctgtct gcggggaact
     4201 tccgccacta tgcagccccg cgatcggccg ggctgccatg gctctcggag agttccttca
     4261 tgcggcggag ccgcgccaga tgctgctccc ggcggaaagt gtagagcccg gcggcggcga
     4321 cgatggcgat gccggcgagc gtcagccgat cggggaaata gccccagacg agaaagctcg
     4381 agatcaggct gaatacgatg atcgcatagc ggaacggcgc gacggcggac acctcgccgg
     4441 tccgcatcgc gatgatgacg cagagctggc cgatgaggga gcatacggca gcgcacgcga
     4501 gaagggcgag cgagcccgca tccggccgcg accagttggc gaacggcgcg acggcgccgc
     4561 tcgtcaagac cactgccagc gccgagagca tcataatcat ggttgccggc acgcgacggt
     4621 ccacctggcg ggtcgccaga tcgcggaccg tcgcgcagag tacggaagcg atgccggcga
     4681 gcgcccacca gttgaaggcg ctcgagcccg gtcggatgat cagcagcacg ccgacgaggc
     4741 cgacgcccgt cgccgtccag cgccgccagc cgaccttctc gccgaggaac acggcggacg
     4801 cggccgtgat cgagagtggc gtcaattgga tgacagacat gaggtcggcg agcggcatgc
     4861 gcgcaaggcc cgactggaag aaccagccgc cgccgacatc gccgccggcg cggagcgcca
     4921 tggcccagga gagcgcctgg cgaatatgct cgagcccacc ccagacgatc atgatgaaga
     4981 tcgccgccga gagcacgacg cttccgcgaa cgaagatcag ctccgatgtc ggcatgtccg
     5041 tcgccgacac gagcttcatg atcgtatcgc cgacgctgaa cgaggccatg gcagcaacca
     5101 tggcgatgat gcccttgagg ttcgccgaca gcggcagccg ctccgggccg ccggggctcg
     5161 cgaggctcat ccgcccgtca cgctcatgtg gcggccgacg gagggccgct tggtgcgccg
     5221 gtcgatgatg aagtcgtggc ccttgggctt gcggcggatg gcgtcgttga tggcggcgcg
     5281 gagggccgca tcgtcagggc tcgcgcggag cggggtcctg aggtccgccg catcctcctg
     5341 tccgaggcac atgtagagcg tgcccgtgca ggtgaggcgc acgcggttgc agctctcgca
     5401 gaaattgtgg gtcatcggcg tgatgaagcc gatccgcccg ccggtctccc tggcctcgac
     5461 atagcgcgcc ggcccgccgg tacgatacgg cacatcggaa agcgtcaggt ggtccatcag
     5521 ccgcgcgcgc acgatcgaca gcggcatgta ctgttcggtt cgatcaccgt cgatgtcacc
     5581 aagcggcatc gtctcgatga aagtgatgtc gaagccttcg ccatgggcgt agcgcacgag
     5641 gtcgaggatc tcgtcctcgt tcacgccctt gagtgccacc gcgttgagct tgatggcgag
     5701 gccggcgcgc ttggctgcga caatgccatc catcacgcct ccgaaatcac cccagcgggt
     5761 gattgccttg aagcggtccg ggcggagcgt atcgagggag atgttgatcc tctcgacgcc
     5821 ggcggccttc agctcgcccg cgaacttcgg cagctggctg ccgttggtcg tgagggtgag
     5881 ctcctcgaga gtgccgcttt ccaggtggcg cgagaggctg cggaagagct gcatgatgtt
     5941 cttgcgcacg agcggctcgc cgcccgtgat gcgcagcttc gagacccccg ccgcgacgaa
     6001 cgcggagcag acgcggtcaa gctcctcgag cgtcaggaga tccttcttcg gcaggaacgt
     6061 catgtgctcg gacatgcagt agacgcagcg gaagtcacac cggtcggtgn nnnnnnnnnn
     6121 nnngtacgtc accgcccgac cgaacgggtc gatcagaggg ggcggcgcta cgtctggctt
     6181 gctcgtactt cccatggtgg tctctggcct gtcggcgatg gaacctacgg aacgagtcag
     6241 cggcttggca tggagtccga cgctggtata cctggatcag tgcccgcgcg gcaatattcc
     6301 cggctccgca tagccgtccc gcagcgacgg ggtcttgccc tcggtcaatc ggcatggctt
     6361 tcacggccag gcccggtgca cgcataaggg ctcagacgat catggacgca cttctacccc
     6421 tcgccactct agcagtcgtt cacatgccgg ccgtgatcag cctcggacaa tagttcctga
     6481 tggtgttgcg gacggcgctc gcgagcggcc ggcgcgctgc ggcggcaggc atgacaggcg
     6541 cgccgacacc ggccagcgtc acctcacccc gggttccgcg ggcgagggcg attcttgtgg
     6601 ggtaccagat agcagcataa cgaattcaat tcccggttgt ggcgagccca gccggatact
     6661 cagctttggc cccgatccag cttgcagcat tgggcggcca cggcacggcc attgctggtc
     6721 cagattcact gaggcacatc cgtgcccacg gacgggtgaa ataaaattcg ctcccggtgg
     6781 atacgcggcg gcctcttcga ccggcaaagc tggcgtcagc cttgccgtca gaaagactgt
     6841 gactgcaaac aacagcaact acgcaattcg agcctcatcg aattctgaat tcgctatcga
     6901 gtagcattac aggcaggtac caagacgacg cgcccgagtc atgcagctct ggatgcattg
     6961 atgccggtag gccgggcgtc tatggtatgc acactgggcg ccacacgagg ccagggcatt
     7021 acaccgaaac ttgtccctgg ttgccgccag tgcaccgtcg cggctgtcgc ccgacgaatg
     7081 atcgagcaag agcacaatgc tgatggctgc gagcaggtac cggaaccggc gcatggggcg
     7141 tctccgtttc ttgaattcag tgatttcgac aaactctgca cgaccaagtc gagcgccaca
     7201 cctgggataa tagaatagtt aaccgtagca tcactgcgag gcccgcgcaa ttggcgcctt
     7261 tgaggcgatt ttccagccct tcatgtcccc gcgtgaggat gagcaacgcg cacacccatc
     7321 agaacgagcc tcggggacgt cgccggtcga gatgtccttc aagtacagga cgggcagcaa
     7381 cgtctcaatc gattgccagc ggcgcatgta tggcgattgg atcgccggcg agaagcggat
     7441 gcggccggga tcgtcggccg ccgccgcccg gtcacgcacg cgcggctgta gcacgtcgat
     7501 cgggccgacc cgacgatatg ccgccatgcc gattcccgcc gtcaccaact ttcggtcgta
     7561 gctcgcgtca accttcgcga aagtgcttcg agagcttgag cccctgcgcc tgatagttcg
     7621 agccgacgtc gcggccatag agaatttcgg gtaactccag gaggcgctcg tagacgaggc
     7681 ggccgacgat ctggccgtcg tcgaggatga acggcacctt gtgcgagcgc acctcgagga
     7741 cgacgcgcgc gccggctcct ccagctgccg catggccgaa gccggggtcc atgaagcccg
     7801 catagtggac gcggaactcg ccgaccagcg ggttgaacgg catcatctcg gccgcatggt
     7861 cgggcggcac gtgcacggct tccttcgagg ctaggatgta gaactcgtcc gggtcgagca
     7921 cgaggcggcc gtccttcggc gccggcagcg cctcccaata gtctgagagc gcgcaagcgc
     7981 cgggtttgtc gacgtcgacg atgccggtat gccgtttcgc ccggtatccg gccggcccct
     8041 cggcccgccg cgcgaggtcg acggaaagcg cgatgccgcc tcggatatcg gctgtggcgc
     8101 cacccgtcac gagtgtgtgg cgcgcgtgaa gccgtgcaag ggcgccgtcg tcgaggacgg
     8161 gatcgccgcg ccggaaacgg atctggctca gcatggagcc gcggcgcgcg acgatgggga
     8221 aggtctgcgg gcagatctcg gcgaacagtc ggccctcgaa gccgaccggg atggtatcga
     8281 aggagggcac gccgtcggcg atcacgcgcg tgaagacgtc gaggcgtccg gtcgagctct
     8341 ttgggttggc cgacgcggag acgtcgtccg gcagtgcgag cgcctcgagg agcggcacga
     8401 cgtagacgca gcccgtctcg aggacggcgc catgcgtgag gtcgatcgcg tgcagcgaga
     8461 gcgcgtcgag cttggtatcg agcgagccgc cgggcgacgg caggaaactc gcgcgcacgc
     8521 gataggccat cgggccgagg cggagatcga ggctcgccgg ctgcacctgg ccttgagacg
     8581 cgggctcggt catgcgtatg gccctggccg caatgagcgc gcgaatggcc tgcgccggca
     8641 ggatgccgct cgtcgcggct gcggctcggt gggtctcagg catggtcgtg ctccggatcg
     8701 cgctcggcgc ggacgagatc acgtcgggcc gccagacgct accacccgcg ccaggatgcc
     8761 tgtcgataaa tggtgtctcg ccaaccttga ggtgctgcag cggtattttt gacgccgcag
     8821 gtgggaaagt actggactgg catgcgcgaa acggccgtgc gctcattttt ttgatcctct
     8881 tttgttctat tttcttgact cgggtggcga gaatgaacta gaacacaaga agaacgaata
     8941 gcgccctgcg tacctcctgc caatcgtacc tgtccctaag gagagctggg cgccgcgtat
     9001 cgttctgcct ctgaagcctg tgctccatcg gtcggccgcg tgctccccct gcacgcgcgg
     9061 ggatcggctg tgacctgggc cttggaggtt cggtcgaagc tcgggtgtcg gcaccggctc
     9121 tttccggttg caggccaccg aataattcct ggcatgtcca tcaccggcga gtagagcatg
     9181 tcgcattgtc acccctcgac aacgcttgcc cgccagggag aggccatcga ggcgccagcg
     9241 ccggacgatc cctcacgtcc atcgccggct gccgagacga cgccggagcc tcgatcgcag
     9301 gcgccgacag cgccgccagc tgttgcgtca agtactgcgt ccgcgttccg ggcgggaccg
     9361 tcaccgtcca tatccctgcc ggtcccgccc gctttttcac gcactccagg ggaggctcgc
     9421 gtcgaccacg cggccctcga ccggctcaaa gccgccatcg aggcgatcga gcgaccctgg
     9481 ctgcggccgt ccccggacac ggccgatggc cgatctcctc ctgatgatcc gagacgcgcc
     9541 ccatggatgt tcggtctcga tgaactcgac gatgggctcg gcgggctcga tccctgcggc
     9601 cttcacgagg tgaggcccga aagtcatcgg gactggggcg cgaccctcac ctttgcgctc
     9661 gctctcgcca tacgccgcat gcgctcggat gccgatcgta cagcgccgct cctctggtgt
     9721 gccacccaag cctcgatgcg tgaggtcggc atgccttatg cgccgggctt gctcgccttg
     9781 ggtctctcgc cggatcgcct cctcatggtg gagacccgcc ggcaggacga tatcc
//
LOCUS       DAORNH010000166         9781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5126_length_9781_cov_2.545240, whole genome shotgun sequence.
ACCESSION   DAORNH010000166 DAORNH010000000
VERSION     DAORNH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_9781_cov_2.545240"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PK264_15835"
     CDS             <1..428
                     /locus_tag="PK264_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD77377.1"
                     /translation="RLAGIYGPGRSAIDQVKGGTARRIIKPGQVFNRIHVADIATVVV
                     AAMERAAALAGGGTATFNVTDDEPAPPEDVIAFAAGLLGVPPPPAIAFEQAELSPMAR
                     SFYGEVKRVRNVRIKAELGVRLAFPTFREGLRALAQDLS"
     gene            complement(421..1185)
                     /locus_tag="PK264_15840"
     CDS             complement(421..1185)
                     /locus_tag="PK264_15840"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD77378.1"
                     /translation="MIQRPDTRLAAKLFASPNIEPRKGNVRPSILLLHYTGMASAASA
                     IQWLATPAAKVSCHYVVDEAGEITQMVAEGMRAWHAGVAHWAGESDINSQSIGIEIQN
                     PGHDLGYPDFPEAQIAAVIALSRDIVLRNSIPPQRVLAHSDVAPMRKIDPGEKFPWGQ
                     LAAAGVGFWPAHLPVPVDEADPGDPAGEGRAIDILDRLAAYGYGIDRSSEQRVQQHRA
                     MIAAFQRHFRPARVDGRVDRSTIATLDILLEALPVS"
     gene            complement(1182..1889)
                     /locus_tag="PK264_15845"
     CDS             complement(1182..1889)
                     /locus_tag="PK264_15845"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRD77379.1"
                     /translation="MGIWQRMAATIGEAARLGSLYEHLKELVTNRVEVGDSGQTGHVA
                     FTIAVIALLAKMARSDGVVVDAEVKAFNEVMRVPAEEARNVQRVFDLAKRDTAGFESY
                     ANQIARAAASDRRLLQHVVEGLLNVAAADGILHPSEDAYLAEVARRFAFTPSQYHHIR
                     SRFVAAGIDSPYAVLGIEPTASDDEIKRRYRELVAKDHPDKLIGRGVPEELVLIATRK
                     LAAINAAYDRIVKERGA"
     gene            2025..2915
                     /locus_tag="PK264_15850"
     CDS             2025..2915
                     /locus_tag="PK264_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD77380.1"
                     /translation="MPGLWIVFTLLAAGGQTLRNALQRELTASLGTTAATHVRFLYGL
                     PFALVFLLLSVLHARQPVPVPGTAALAWLLGGALSQIAGTAMLLAAMRERSFVVITAY
                     SKTEPVLVALFGLAVLGDALSWPLALAIAIATAGVLVVSWPKAAAAETFSPSAVGYGL
                     ASAAMFGLAAIGYRGGVKAMPGDALYLNASTLLVIALAVQTTLLTLYLLVAERQNLIA
                     LLRSWRTSLKAGFLGAFASQMWLLAFTLETAAKVRTLALVEILMAQVISRSMFRQSLA
                     SREGLGIALIVAGVALLLNL"
     gene            complement(2910..3851)
                     /locus_tag="PK264_15855"
     CDS             complement(2910..3851)
                     /locus_tag="PK264_15855"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRD77381.1"
                     /translation="MIDALSIVLPVFGLIAIGLAAAKLGVLTEASEKGIADFAFTFAM
                     PALLFRTMATVKVPDVAPVSLWLAFLSTAASVWVLSTLLTILVLKRPAPDAAPISMAA
                     TFGNNVMLGIPLAVAAFGDAALAPAALLLSVQTPLYWLAATLHLQWADRAVGVPLSSV
                     IATMLRDLSRNPVLMAIILGSLWRTTGLGLATPVERTLALLGQAGIPCALIALGMSLA
                     RFKIAGQLPTLTMIIVLKIVAMPAVGFVLTKYVFDLPPLALAVALLFMATPTGANAFL
                     FASRYGRAVNSTAAAVTVTTALGVVTTTAVIWLLKSS"
     gene            4116..4481
                     /gene="mscL"
                     /locus_tag="PK264_15860"
     CDS             4116..4481
                     /gene="mscL"
                     /locus_tag="PK264_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRD77382.1"
                     /translation="MIQEFKEFALKGNVMDLAVGVIIGAAFGKIVDSLVKNIIMPIVA
                     AAGGSPKFDFKVGPIEAGAFVTTVIDFVILAFCIFMMVKLMNAARRRMEKPATPAAPA
                     APPASETYLKEIRDLLAKK"
     gene            4640..5617
                     /locus_tag="PK264_15865"
     CDS             4640..5617
                     /locus_tag="PK264_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77383.1"
                     /translation="MLAFAIRRLLEAMLVMLVVAALAFYLFAYIGDPVSQLVGQDATL
                     EDRIKLRSDLGLEDPWIVQFGRFVASAVRFDFGVSYQFKQPVTTLITERFPATMELAF
                     AASLFALLIGIPMGVYTGIHRHAWTSKLFLTVSLVGISLPTFLIGILLIYVFAVILGW
                     LPSSMRPGVVQIGFWKTSFLTWTGIKAMILPAITLGLFQMTLITRLVRSEMLEVMRTD
                     YIKFARARGLTNRAVNFGHALKNTLVPVVTIIGLQVGAIIAFAIITETVFQWPGMGLL
                     FIQAVQNADIPIMSAYLMLVAFLFVVINFMVDLLYAWIDPRIRIVRREP"
     gene            5614..6594
                     /locus_tag="PK264_15870"
     CDS             5614..6594
                     /locus_tag="PK264_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77384.1"
                     /translation="MTSESSSPASEDRAAPRALPRPQSRLAKLLDSDLFYSFKRSRLV
                     VVAATLTLLMVGAALLAPLIAPHNPYDLKSLVLLDADTPPVWDAKGDRRFLLGTDDQG
                     RDILSTILYGTRSSLLVSVLAVGLALLLGVSAGLVAGYFGGWADTIIMRLADVQLTFP
                     AILTALMVDGVAQVIFKGHKSGGGGAFWILVVAIGLAFWVQYARTVRSVTLVERNKEY
                     VQAARLIGIGKIKIMFQHVLPNVLSPVLVIATINLALAIITEATLSFLGVGLPPTEPS
                     LGTMIQIGNKYLFSGSWWMVAFPGITLALLVLSVNLLGDWLRDALNPKLR"
     gene            6659..>6941
                     /locus_tag="PK264_15875"
     CDS             6659..>6941
                     /locus_tag="PK264_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD77385.1"
                     /translation="MTKRMRAAAMLAALIAASPIAISLADAKTLRWARSQDALTLDPH
                     AQNEGATFNLLHSIYEPMILRDRDGKLLPTLALSWGVTSDPTVWEFKLRP"
     assembly_gap    6942..6957
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6958..8272
                     /locus_tag="PK264_15880"
     CDS             <6958..8272
                     /locus_tag="PK264_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD77386.1"
                     /translation="FHAGQTMTADDVVFSLGRAQQPTSGVKGLLTAIDSVSKVDDLTI
                     RIKTKGPNPLLPSYLTNIFVMSKAWAEANNSVTVQDFKEKKDNFAVRNANGTGPYTLV
                     SREQDVRTVMKRFDGYWGKGMFPLGVTEINYVTIKSDATRIASLLSGEVDFVQDVPVQ
                     DIDRLAKTANLRVNFGPENRSIFLGFNVGSGDLASSDIKGKNPFADVRVRRAVSMAID
                     REAIKAKVMRGQSVPSGMIAPPFVNGYTKELDKVPAVDVDGAKKLLAEAGYAAGFSVA
                     LNCPNDRYVSDEAICQAVVAMLARIGIKVTLVSQSKTKHFPLIEKNPPETDFFLLGWG
                     VPTYDSHYIFSFLYHSRSGKEGSFNGTRYANPDMDKLIAGLTSETDQAKRNATIAEIW
                     KKVHADTVYAALHNQTLAFAMSKDWDLPTSPENAVRIKFFGPVAK"
     gene            8383..>8857
                     /locus_tag="PK264_15885"
     CDS             8383..>8857
                     /locus_tag="PK264_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77387.1"
                     /translation="MAEPVLSVRNLRVEFPTRRGTLVAVDNVSFDIAAGEVLGVVGES
                     GAGKSMTGSAVIGLIEPPGRIAGGEVRLAGRRIDNLPYDEMRRIRGRRIGMVFQDPLT
                     SLNPLYRVGDQIIETIRTHLPVSEGEARARAIALLQEVGIQGAERRIDGYPHEFSG"
     assembly_gap    8858..8897
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8898..9390
                     /locus_tag="PK264_15890"
     CDS             <8898..9390
                     /locus_tag="PK264_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HRD77388.1"
                     /translation="AEPELVVADEPTTALDVSIQAQIIGLLKRLCRERGTAVMLITHD
                     MGVIAETADRVAVMYAGRIAEIGPVRDVIQRPHHPYTKGLMGSIPTLEAAASRLVQIP
                     GSMPRLTAIPRGCAFNPRCREVIERCRLERPEPVAIGASAVACWVAAARAKAGTTAES
                     IDA"
     gene            9387..>9781
                     /locus_tag="PK264_15895"
     CDS             9387..>9781
                     /locus_tag="PK264_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77389.1"
                     /translation="MSGPLVEVRGLARTFDVSKPWLNRVIEREERKLLRAVEDVSLEI
                     GAKETFALVGESGSGKSTVAKMIVGLLKPTAGSVAIAGVPMWDQGGRGGSRESGMAAI
                     PGERVGPAPQAGESERRLLRRRIQMIFQDP"
BASE COUNT         1602 a         3265 c         3129 g         1729 t
ORIGIN      
        1 tccggctggc gggcatctac ggacccggcc gcagcgcgat cgaccaggtg aaggggggca
       61 cggcgcggcg catcatcaag ccggggcagg tgttcaaccg catccatgtc gccgacatcg
      121 cgaccgtcgt cgtggcggcc atggaacgcg cggcggcgct tgccggtggc gggacggcga
      181 cattcaacgt gacggacgac gagccggcgc ctccggagga cgtgatcgcc ttcgcggccg
      241 gccttctcgg cgtgccgccg cctccggcaa tcgccttcga gcaggccgag ctctcgccca
      301 tggcgcggag cttttacggc gaggtgaagc gcgtccgcaa tgtgcggatc aaggccgaac
      361 tcggcgtgcg cctcgcattc ccaacgttcc gcgagggcct cagagcgctg gctcaggatc
      421 tcagctgacg ggcagcgcct cgagcaggat atcgagcgtg gcgatggtcg agcgatcgac
      481 gcgcccgtcg acgcgcgcag ggcggaaatg gcgctggaag gccgcaatca tcgcccggtg
      541 ctgctgcacg cgctgctcgc tcgaccggtc aatgccgtag ccataggctg cgaggcgatc
      601 gaggatatcg atcgcgcgtc cctcgcccgc gggatcgccg ggatcggcct cgtccaccgg
      661 caccggcagg tgtgccggcc agaagccgac gcctgccgcc gcgagctggc cccaagggaa
      721 tttctcgccg ggatcgatct tgcgcattgg tgcgacgtcc gaatgggcga ggacgcgctg
      781 aggcgggatc gagttgcgca agacgatgtc gcgcgagagc gcgatcacgg ccgcgatctg
      841 cgcctcgggg aaatccgggt agccaagatc gtggccgggg ttctgaatct cgatgccgat
      901 cgattgcgag ttgatgtcgc tctcgcccgc ccagtgcgcg acgcccgcgt gccatgcgcg
      961 catgccctcg gcgaccatct gcgtgatctc gccggcttcg tcgacgacgt agtggcacga
     1021 gaccttggcg gcgggcgtgg cgagccactg gatcgcagag gcggccgacg ccatgccggt
     1081 atagtgcagg aggaggatgc tcggccgcac gttgcccttg cgcggctcga tgttgggcga
     1141 ggcgaagagc ttggccgcga gccgcgtgtc gggccgttgt atcacgcgcc gcgctccttc
     1201 acgatgcggt cgtaggcggc gttgatggcg gcgagcttgc gcgtcgcgat cagcaccaac
     1261 tcctccggca cgccgcggcc gataagcttg tcggggtgat ccttggcaac gagctcacgg
     1321 tagcgccgct taatctcgtc gtcgctggcc gttggctcaa tgccgagcac cgcatagggg
     1381 ctgtcgatgc cggccgccac gaagcgtgag cggatgtggt gatattgcga cggcgtgaag
     1441 gcgaagcgcc tcgcgacctc ggcgagatag gcatcctcgc tcgggtgcag gatgccgtcc
     1501 gcagccgcga cattgaggag gccttccacg acatgctgca gcaggcggcg atcgctggcg
     1561 gcggctcgcg ctatctgatt ggcgtagctc tcgaagccgg ccgtatcccg cttggcgagg
     1621 tcaaacacgc gctgaacgtt gcgggcctct tccgccggca cccgcatcac ctcgttgaag
     1681 gctttgacct ccgcatcgac gacgacgccg tcggagcgcg ccatcttggc aagtagtgcg
     1741 atcacggcaa tcgtgaaggc gacgtgcccg gtctggccgc tatcgcccac ctcgacgcgg
     1801 ttcgtcacga gctccttcag gtgctcgtag agcgagccga ggcgcgctgc ctcgccgatc
     1861 gtcgcggcca tgcgttgcca gatacccatg ctgagtgcat atcctcgttg ctggccccgc
     1921 caaagggcgc cgcgaccatc gtggcgtttg gacgcatatg gcatcgaacg ccttccacgc
     1981 aggattttga cgttcaaggc gaggccgagc ttggggaggg cagtgtgccg ggtctgtgga
     2041 ttgtctttac attgttggct gccggcgggc agacgctgcg caatgccttg cagcgcgagc
     2101 tgacggcctc gctcggcact acggctgcga cgcatgtgcg cttcctctac ggcttgccgt
     2161 tcgcgctcgt gttcctgctc ttatctgtgc tccacgcgcg ccaacccgtg cctgtgccgg
     2221 gcacggcggc gctcgcctgg ctgctcggcg gcgcactgtc gcagatcgcg ggcacggcaa
     2281 tgctgctcgc tgcaatgcgc gagcgctcgt tcgtggtgat cacggcctat tccaagacgg
     2341 agcccgtgct ggtcgcgctg ttcgggctcg cggtgctcgg cgatgcgctc tcctggccgc
     2401 tggcgctcgc cattgccatc gcgacggcgg gtgtgctcgt cgtgtcgtgg ccgaaagcgg
     2461 ccgccgccga gacgttctcg ccgagcgcgg tcggttacgg tctcgcctcg gcggccatgt
     2521 tcggccttgc ggccatcggc taccgcggcg gggtgaaggc gatgcccggc gacgcgctct
     2581 atctcaatgc ctcgacgctc ctcgtgatcg cgctcgccgt gcagacgacg ctgctcacgc
     2641 tctatctgct ggtcgccgag cgtcagaatc tcatagcgct cctgcgatcg tggcggacgt
     2701 cgctgaaggc cgggtttctc ggcgcgttcg cctcgcagat gtggctgctc gcgttcacgc
     2761 tcgagacggc ggccaaggtg cgcaccctcg ccctcgtcga gatcctgatg gcgcaggtga
     2821 tttcccgcag catgttcagg cagagtcttg cctcgcgcga gggcctcggc atcgcgctga
     2881 tcgttgccgg tgtcgcgctg ctgctcaacc tatgagctct tgagcagcca gatgaccgct
     2941 gtcgtcgtta cgacgccgag cgctgtcgtg accgtgaccg cggcggcagt cgagttcacc
     3001 gcgcggccat agcggctcgc gaacaggaag gcgttggcac cggtcggcgt tgccatgaac
     3061 aggagtgcta ccgcgagcgc cagcggcggc agatcgaaga cgtacttggt gagcacgaaa
     3121 ccgaccgccg gcatggcgac aatcttcagg actatgatca tcgtgagcgt cggcagttgg
     3181 cccgcgatct tgaagcgtgc gaggctcatg ccaagggcga tcagcgcgca gggaatgccc
     3241 gcctgcccga ggagcgcgag cgttcgctcg acaggcgtgg cgagcccgag acccgtcgtg
     3301 cgccagagcg agccaaggat gatcgccatg agaacgggat tgcgggagag atcgcgcagc
     3361 atggtcgcga tcacgctcga cagcggcacg ccgacggcgc gatcggccca ttgcagatgc
     3421 agcgtcgctg caagccagta gagcggcgtc tgcaccgaga gcagcagcgc ggccggggcg
     3481 agcgcggcat cgccgaaggc agcgacggcg agcggaatgc cgagcatcac gttgttgcca
     3541 aaggtcgctg ccatcgagat gggtgcggca tcgggcgccg gccgcttcaa gacgagaatg
     3601 gtcagaagtg tcgagagcac ccatacgctc gccgctgtcg agaggaacgc aagccagagc
     3661 gataccggcg ccacgtcggg caccttcacc gtcgccatcg tccggaacag cagcgccggc
     3721 atcgcgaagg tgaaggcgaa gtcggcgatc cccttttccg aagcctcggt caggactccc
     3781 agcttggccg ccgcgagtcc gatcgcgatc aggccgaaga ccggcagaac aatcgagagc
     3841 gcgtcgatca tggaatgccc ctatctccgt cgactacgat catgctgcca ggagtgccgg
     3901 ctcacgcaca attacccgcc ggctcgtcca ttcacactca tctcactcga ctgttgttga
     3961 ccttccgcat gccgattagg ctccgggccg ggcgggtgat tcgcgtgtcg tcaatagcga
     4021 gcctgccggc aggcatccgg ctgcctcgtg tcaagagcag ctgagaggcc ggaaatctac
     4081 ggggcacggt aacaggacag gggctgatca tgggcattat ccaggaattc aaggagttcg
     4141 cgctcaaggg caacgtgatg gaccttgctg ttggcgtgat catcggcgcc gctttcggca
     4201 agatcgtcga ttcactcgtc aagaacatca tcatgccgat tgtcgccgca gccggcggca
     4261 gcccgaagtt cgacttcaag gtcggcccga tcgaggccgg cgctttcgtg acgacagtga
     4321 tcgatttcgt catcctcgca ttctgcatct tcatgatggt gaagctcatg aacgcggccc
     4381 gccgtcgcat ggagaagccc gcgacacctg cagcgccggc cgccccgccg gcatccgaga
     4441 cctacctgaa ggagatccgc gacctgctcg ccaagaagta agcgagtctc gccgatctga
     4501 tcttgcgatg agccccgctg gtcaccagcg gggctttcac ttatgtgttt ctcgacacgc
     4561 cccgcattga catgcggcgc ggcgacgcga tcaatagccg cttcggtact tggcctgtcg
     4621 ctcagggggc gcctcgcgca tgctcgcctt cgccatccgc cgcctgctgg aggcgatgct
     4681 cgtcatgctc gtcgtggcgg cgctcgcgtt ctacctgttc gcctacatcg gcgatccggt
     4741 cagccagctc gtcggccagg acgcgactct cgaggatcgg atcaagctgc gcagcgacct
     4801 cggccttgag gatccctgga tcgtccagtt cggccgcttc gtcgcaagcg ccgtccgttt
     4861 cgatttcggc gtctcctacc agttcaagca gcccgtcacg acgctgatca ccgagcgctt
     4921 tccggcgacc atggagctcg cgttcgcggc ctccctattc gcgctgctga tcggcatccc
     4981 gatgggcgtc tatacaggca tccaccgtca cgcctggacc tcaaagctct tcctgacggt
     5041 gagcctcgtc ggcatctcgc tgccgacctt cctgatcggc atcctgctca tctacgtttt
     5101 cgccgtgatc ctcggctggc tgccgtcctc gatgcggccg ggcgtcgtgc agatcggctt
     5161 ctggaagacg agcttcctca cctggaccgg catcaaggcg atgattctgc ccgcgatcac
     5221 gctcggcctc ttccagatga cgctgatcac gcggctcgtg cgctcggaga tgctcgaggt
     5281 gatgcgcacg gactacatca agttcgcgcg cgcccgcggt ctcacgaacc gcgccgtcaa
     5341 cttcggccat gcgctgaaga acacgctggt ccccgtcgtc accatcatcg ggcttcaggt
     5401 cggcgccatc atcgccttcg ccatcattac ggaaacggtg ttccagtggc cggggatggg
     5461 gctcctgttt atccaggcag tgcagaacgc cgacatcccg atcatgtcgg cctacctgat
     5521 gctggtcgcg ttcctgttcg tcgtaatcaa cttcatggtt gatctcctct atgcctggat
     5581 cgacccgcgc atccgcatcg tgaggcgtga gccatgacca gcgagtcctc cagccccgcc
     5641 agcgaggatc gcgccgcgcc tcgcgcgctg ccgcggccgc aatcgcggct cgcgaaactc
     5701 ctcgacagcg atctcttcta cagcttcaag cgctcgcgcc tcgtcgtagt cgcggcaacg
     5761 ctcacgctgc tgatggtggg tgcggcgctg ctcgccccgc tcattgcgcc acacaatcct
     5821 tatgatctga agtcgctcgt cctcctcgac gccgatacgc cgcccgtatg ggatgccaag
     5881 ggcgatcgcc gcttcctgct cggcaccgac gatcagggcc gcgacatcct ctcgacgatc
     5941 ctctacggca cgcggtcatc tcttctggtc agcgtcctcg ctgtcggcct cgcgctcctg
     6001 ctcggcgtct cggcgggcct cgttgccggc tatttcggcg gctgggcgga taccatcatc
     6061 atgcggctcg ccgacgtgca gctgacattc ccggcgatcc tgaccgcgct gatggtcgac
     6121 ggcgtcgccc aggtcatctt caaaggccac aagtccggcg gcggcggcgc cttctggatc
     6181 ctcgtcgtcg ccatcggcct cgccttttgg gtgcaatatg cgcgaaccgt gcgcagcgtg
     6241 acgctggtcg agcgcaacaa ggagtatgtg caggccgccc gcctcatcgg catcggcaag
     6301 atcaagatca tgttccagca cgtgctgccg aacgtgctct cgcccgtcct ggtgatcgca
     6361 acgatcaacc tcgccctcgc catcatcaca gaagcgacgc tctctttcct cggcgttggc
     6421 ctgccgccga ccgagccgtc gctcggcacg atgatccaga tcggcaacaa gtatctcttc
     6481 tcgggctcgt ggtggatggt cgcgttcccc ggcatcacgc ttgctctgct ggtgctctcg
     6541 gtcaacctgc tcggcgactg gttgcgcgat gcattgaacc cgaagctcag gtaagctcga
     6601 cagcaattca acgtcggtcg ggcatccccc gccacgaccg aaggcaagga ggactgccat
     6661 gacgaaacga atgcgcgctg cagcaatgct cgcggcgctg atagcggcat caccgatcgc
     6721 gatttcgctc gctgatgcca aaacgctgcg ctgggcgcgc tcgcaggatg cgctgacact
     6781 cgacccgcac gcccagaacg agggcgcgac gttcaacctt ctgcacagca tctacgagcc
     6841 gatgatcctg cgcgatcgtg acggcaagct gctgccgacg ctggcgctct cgtggggcgt
     6901 gacctccgat ccgacggttt gggaattcaa gctccgcccc annnnnnnnn nnnnnnngtt
     6961 ccacgccggc cagaccatga cggccgatga tgtcgtcttc tcgctcggcc gcgcgcagca
     7021 gccgacctcg ggcgtgaagg gcctgctcac cgcgatcgac agcgtttcga aagtcgacga
     7081 tctcacgatc cgcatcaaga ccaagggtcc gaacccgctg ctgccctcct acctcacgaa
     7141 catcttcgtg atgagcaagg cctgggccga ggcgaacaac tcggtcacgg tgcaggactt
     7201 caaggagaag aaggacaact tcgccgtccg caatgccaac ggcaccggac cctacacgct
     7261 cgtcagccgc gagcaggacg tgcgcaccgt aatgaaacgg ttcgacggct actggggcaa
     7321 gggcatgttc ccgctcggcg tcaccgagat caactacgtc accatcaagt cggacgcgac
     7381 ccgcatcgcc tcgctgctct cgggcgaggt cgactttgtg caggacgtgc ccgtgcagga
     7441 catcgaccgg ctcgccaaga cggccaacct gcgcgtcaac ttcgggccgg agaaccgttc
     7501 gatcttcctc ggcttcaatg tcggcagcgg cgacctcgcc tcctccgaca tcaagggtaa
     7561 gaacccgttt gccgacgtcc gcgtgcgccg cgccgtcagc atggccatcg accgcgaggc
     7621 catcaaggcg aaggtgatgc gcggccagtc ggtgccgtcc ggcatgatcg ccccgccgtt
     7681 cgtcaatggc tacacgaagg agctcgacaa ggtgccggcc gtcgatgtcg acggtgccaa
     7741 gaagctgctc gctgaggcgg gttacgcggc cggattctcc gtcgcgctca actgcccgaa
     7801 tgaccgctac gtctccgacg aggcgatctg ccaggccgtg gttgcgatgc tcgcccgcat
     7861 cggcatcaag gtgacgctcg tttcgcagtc gaagaccaag cacttcccgc tgatcgagaa
     7921 gaacccgccc gagacggatt tcttcctgct cggctggggt gtcccgactt acgacagcca
     7981 ctacattttc tcgttcctct atcattcgcg gagcggcaag gaaggctcgt tcaacggcac
     8041 gcgctacgcc aaccccgaca tggataaact gatcgccggc ctcacctccg agaccgatca
     8101 ggcgaagcgc aacgcgacga ttgccgagat ctggaagaag gtgcacgccg acacggtcta
     8161 tgcggcactc cacaaccaga cactcgcctt cgccatgtcg aaggactggg acctgccgac
     8221 ctcccccgag aacgccgtgc gcatcaagtt cttcggaccg gttgcgaagt aagcttgggt
     8281 gacagtcgaa cgcccggcgc gcctcgatct ggcgcgccgg gcacacgatc aggatcgccc
     8341 tcgggcggcc cgtggcaagc aatccgaggc gccccgtcag gcatggccga gcccgtccta
     8401 tccgtccgca atctccgggt cgaatttccg acccgcaggg gcacgctcgt cgctgtcgat
     8461 aacgtgagtt tcgacatcgc cgcgggcgag gtgctcggcg tcgttggcga atccggtgcc
     8521 ggcaagtcga tgaccggctc cgccgtcatt ggcctcatcg agccgccagg gcggatcgcc
     8581 ggcggcgaag tgcggctcgc tgggcggcgc atcgacaatc tcccctacga cgagatgcgc
     8641 cgcatccgcg gccggcgcat cggcatggtc ttccaggatc ctctgacgag cctcaacccg
     8701 ctctatcgcg tcggcgacca gatcatcgag accatccgca cacacttgcc cgtctccgaa
     8761 ggcgaggcgc gggcgcgggc gatagcgctt ctccaggagg tcggcattca gggtgccgag
     8821 cggcgcatcg acggctatcc gcacgagttc tcgggcgnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnncgc cgagcccgaa ctcgtcgtcg ccgacgagcc gacgaccgcg
     8941 ctcgacgtgt cgatccaggc gcagatcatc ggcctcctga agcggctctg ccgcgagcgc
     9001 ggcaccgccg tcatgctgat cacccacgat atgggcgtga tagccgaaac ggcggatcga
     9061 gttgccgtca tgtatgcggg ccgcatcgcc gaaatcggcc cagtgcgcga tgtcatccaa
     9121 cggcctcacc acccgtacac gaaaggtctc atgggctcga tcccgacgct cgaggccgcc
     9181 gcgagccggc tcgtgcagat cccgggctcc atgccgcggc tgactgcgat cccgcggggc
     9241 tgcgccttca atccgcgttg ccgcgaagtc atcgaacgct gccggctcga gcgtccggag
     9301 cccgtcgcca tcggcgcgtc tgctgttgcc tgctgggtcg ccgccgctcg cgctaaggca
     9361 ggcacgaccg ccgagagtat cgatgcatga gcggaccgct ggtcgaggtg cgtggccttg
     9421 cccgcacgtt tgatgtctcc aagccctggc tcaaccgtgt catcgagcgg gaggagcgca
     9481 agctgctgcg cgccgtcgag gacgtctcgc tggagatcgg ggccaaggag acgtttgcgc
     9541 tcgttggcga atccggctcg gggaagtcga ccgtcgccaa aatgatcgtc ggcctgctca
     9601 agccgacggc aggcagcgtc gccatcgccg gcgtgccgat gtgggatcag ggcgggcgcg
     9661 gcggaagtcg cgagagcggg atggcagcga tcccgggcga gcgcgtcgga ccggcacccc
     9721 aagctggcga atcggagcgg cgtctcctgc gccgccgcat ccagatgatc ttccaggacc
     9781 c
//
LOCUS       DAORNH010000167         9765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5136_length_9765_cov_2.645005, whole genome shotgun sequence.
ACCESSION   DAORNH010000167 DAORNH010000000
VERSION     DAORNH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5136_length_9765_cov_2.645005"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PK264_15900"
     CDS             <1..823
                     /locus_tag="PK264_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD77390.1"
                     /translation="EMLMRHKDVAFILATGGASMVKAAYSSGKPAIGVGPGNAPTFVA
                     PDADLTHAAASIVASKSFDNGLICGSEHNLVTTAATREGLVASLAAHGAAVLAPDEVT
                     KLTQCVVPPGEFRFRPEVVGQSAAAIAKVAGITRPHPIGLLVVPTDDAKPGNPWAHEK
                     MAPMVSLFTVADTEAAIALAQRLLEIEGKGHTAIIHTRDRALARRYGLAMPASRILVN
                     SPGSQGIVGHTTNLKPSFTLGCGTFGGNSTTDNVTFAHLLNVKRLAEFVAPPSSS"
     gene            835..960
                     /locus_tag="PK264_15905"
     CDS             835..960
                     /locus_tag="PK264_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77391.1"
                     /translation="MELAREIVASTLDADWQAMIAMCRAGDPQGRFMSPSSASLV"
     gene            1108..2310
                     /locus_tag="PK264_15910"
     CDS             1108..2310
                     /locus_tag="PK264_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD77392.1"
                     /translation="MADPVGVAGPAGKVVIRNIGLLLSGDLARPILDADAIVTDKGRI
                     TAVGRLKDLDTSHSSVTIDAKGTTVAPGLIDSHVHPVFGDWTPRQSQIGWIDSFLNGG
                     VTTMISAGEVHLPGRPRDIVGVKALAITAQRAFTNFRPGGVKLHAGAPTIEQGMEEAD
                     FAEMAKAGVKLLGEVGLGTVKAGAEAKKMVAWARKYGIQSTIHTGGPSVPGSGYIDAD
                     TVLEADADVIGHINGGHTALPLKQIECLCGQCSRAIEVVHNGNEYAGLHAVKIAQQLK
                     QLDRVILGTDSPAGSGVQPLGILRMIALIASFSDLAPETVVCFATGNTARTRDLDCGL
                     IEPGRAADFVIMDRAQHSAGRDILASIKGGDLPGIGMVIIDGVVRCGRSRNTPPADRI
                     PEVITHAH"
     gene            2375..>2953
                     /locus_tag="PK264_15915"
     CDS             2375..>2953
                     /locus_tag="PK264_15915"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRD77393.1"
                     /translation="MSTPSTHKPLDAAASDPKALRWMTGFPPAPDVAITFADGTFREF
                     PQSRWAYSNYRQFLPTKTVWRGRGPVSELKRDEQPLGILAYTTMDGRRITFDQALAET
                     YADGIAILHRGRLVFERYFGALEPHKPHIAMSVTKSFTGTLAAALVAEGRIDPKARVP
                     EYVPELAQSGFGDATVGEVMDMTTAIDYAEIYT"
     assembly_gap    2954..2963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2964..3629
                     /locus_tag="PK264_15920"
     CDS             <2964..3629
                     /locus_tag="PK264_15920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRD77394.1"
                     /translation="DKNSDVWAMRRANGTAPPEPGAPPTSLLHYLTTLKKEGRHGEAF
                     TYRTPNTDVLGWIVRRVTGQGLAELVSERIWQQIGAEEDAHYTVDRLGTEYGGGGLNT
                     TVRDLARFAEMMRCRGRFNGRQIIPAAAVDDIAKGADPKKFAPAGYQTLPGWSYHHQW
                     WVSHDSHGVYMARGIHGQSIYVDPKAEMVIARYASHPMAGNVNNDPVTLPAFRAVALA
                     LMG"
     gene            complement(3640..4146)
                     /locus_tag="PK264_15925"
     CDS             complement(3640..4146)
                     /locus_tag="PK264_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HRD77395.1"
                     /translation="MKRSKERQLTKEPSDTALLDRTDRRLLRLLQDDGRISNADLAAA
                     ANVSPATCHRRLARLFADGFVRGVRAEVAPHRVERGTLVLVGVVLDRSTKDSFAAFEA
                     AARKLRVVLDCHLVAGDFDYFLKIRVRDMADFNRLHGEQLIGLPGVRQTRTFFVMKEV
                     VDNAPLEF"
     gene            4256..5275
                     /locus_tag="PK264_15930"
     CDS             4256..5275
                     /locus_tag="PK264_15930"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HRD77396.1"
                     /translation="MLKLDKFPRYSLTFGPTPIEFLPRMTAALGGKVQIYAKRDDCNS
                     GLAFGGNKLRKLEYIVPDAIASNADTLVSIGGVQSNHTRMVAATAAKIGMKCVVIQES
                     WVPHEDAVYDRVGNILLTRLMGADSRIVSDGFDIGIRKSWGEAIESVKKAGGKPYAIP
                     AGASVHKYGGLGYVGFAEEVRAQEKQMGVRFDYIIVCVVTGSTQAGMIVGFAADDRAS
                     RVIGIDGSGTPKPLRAQVRQIVDNTAELVELGRKVRDDEIVILEDYAYPAYGVPSAET
                     NEAIRFAARTEAMITDPVYEGKSMQGMMDLVKKGYFPAGSKVLYAHLGGAPAINGYSY
                     TYRNG"
     gene            complement(5323..6105)
                     /locus_tag="PK264_15935"
     CDS             complement(5323..6105)
                     /locus_tag="PK264_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77397.1"
                     /translation="MLVQYADGELDEAKRREIEAYLASDPKAAELVNRLQKSAALLKQ
                     AFEVPASSPVPDRLIAVLQESPAARPGVVDAEHTTTITPIGRRRLSQRGMFLPMAMAA
                     SLALVVGAGVGFGLRDFRSPSISTVIAAGPVASGSALARLLHSRTTGEGEQIGAGARR
                     IEVVGTFRDRTGRPCREIEQLDGKDTSTAPELAAIACADGAGGWVVEGVVRVAASATP
                     DGKFRPSGADEKDALKALLIALGAGAAVSPSEERGLIQRSWK"
     gene            complement(6543..7058)
                     /locus_tag="PK264_15940"
     CDS             complement(6543..7058)
                     /locus_tag="PK264_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD77398.1"
                     /translation="MAIQASTTESTLRQEIITLLPRLRRFAYALSGSPEQADDIVQAA
                     CLRALSRLHQFTPGTRLDSWMFRIVQTTFVDQGRARARRPTASDDEALGNVADDARIH
                     ERTEARAALAIVRDEMAKLPEEQRIVLSLVAIEGLSYQEAADTLEIPIGTVMSRLSRA
                     RRRLADAIDPA"
     gene            complement(7083..8405)
                     /locus_tag="PK264_15945"
     CDS             complement(7083..8405)
                     /locus_tag="PK264_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRD77399.1"
                     /translation="MSPFGVTPTLADDGDGGGAGPGGGSAGDSGSGGDWMAPARGGTR
                     ASKCVCLVIFCACKQTRARRSKSTPSKEARPAAPPVRHELVVAAPTADASNAVRSAGF
                     ELLDQRDNRLLGSSVLRVRAPAGMGRDTARRRIAAAVPGSTIANNDLYQRNRGSYKAA
                     GEGCGQTCSAFTLTAWSDGIGKCSARVRIGMIDTGVDQSHPALTTARITVKDHRASGR
                     KPSSADHGTGVASLLVGARNSEVVGLVPDSQLFAADAFHSEGAEMAADVFALVAALDW
                     LVEVDVVTINMSLSGPDNAVLLAAIRKTIERGVVIVAASGPPASGSSQGYPARYDGVV
                     AVGAIDDRMRSSRLSTRGKHIAFAAPGVGLLTAAPAGRLQRVDGTSFASPFVAAAFAM
                     GVATDPARPTLASLTERLAGSARDLGAPGRDPVYGWGLIQFTAFAGCR"
     gene            complement(9115..>9765)
                     /locus_tag="PK264_15950"
     CDS             complement(9115..>9765)
                     /locus_tag="PK264_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77400.1"
                     /translation="LAAGGNAGAASYAVGPSRPYPTLQALLAGVTLQPGDLVEVDGNA
                     TYAGGVILRDSGSAALPVTIRGLRVNGQRPILSGGSNTIEFRQSNHVVFEGFEVTGGT
                     SRCVFVPAAFITLRDLLVRRAFVGQLNLRGSGCSRSGAAWVAVRCSDGPGRAGGNHGG
                     RGRAHLSELQISDKVARLRRTSVCLLSASGTCADHRRSSHRMPRCCVRHRVACPEW"
BASE COUNT         1720 a         3418 c         3127 g         1490 t
ORIGIN      
        1 cgagatgctg atgcgacaca aggacgtggc cttcatcctc gcgaccggcg gcgccagcat
       61 ggtcaaagcg gcctacagct ccgggaagcc cgcgatcggc gtcggcccgg gcaatgcgcc
      121 gaccttcgtt gcgcccgatg ctgacctcac gcacgccgcg gccagcatcg tcgcctcgaa
      181 gtcgttcgac aacggcctca tctgcggctc ggagcacaac ctcgtgacga ccgctgcgac
      241 cagagagggc ctcgtcgcgt ccctcgcagc gcacggggca gctgtcctcg cgcccgacga
      301 ggttacgaag ctcacgcagt gcgtcgtgcc gccgggcgag ttccgcttcc ggccggaagt
      361 ggtcggccag agtgctgccg ccatcgccaa ggtcgccggc atcacgcggc cacatccgat
      421 tggacttctc gtggtgccga cggacgatgc caagcccggc aacccctggg cgcacgagaa
      481 gatggcaccg atggtgagcc tcttcacagt cgccgacacg gaggccgcca tcgcgctcgc
      541 ccagcgcctc ctcgagattg agggcaaagg gcacacggcc atcattcata cgcgcgaccg
      601 tgctctggca cgacgctacg gcctggcgat gccggcgagc cgtatcctcg tcaactcgcc
      661 gggaagccaa ggcatcgtcg gtcacaccac caacctgaag ccgtcgttca cgctcggctg
      721 cggcacgttc ggcggcaact cgacgaccga caacgtcacg ttcgcgcacc tcctcaatgt
      781 gaaacgcctc gccgagttcg tcgcgccgcc atcgtcgagt tgaaccaccg gccgatggag
      841 ctcgcccgcg agatcgtcgc aagcacgctc gatgccgatt ggcaggcgat gatcgccatg
      901 tgccgcgccg gcgatccgca aggccggttc atgagcccgt cttccgcgtc gctcgtctga
      961 caccgtggtc gcccgatcca tgcgtgccgc cgcgccccga tctgccggca atcgcgtggc
     1021 gggtgctcga gcgacctgct aatggaccat gatgcggccg agccggtgcc cgggcggatc
     1081 gcgcgatgtc aacgcaggga gatcctcatg gccgatccgg tgggagttgc agggccggcc
     1141 ggcaaggtcg tcatccgcaa tataggcctc ctgctttcgg gcgacctcgc caggcccatc
     1201 ctcgacgccg acgcgatcgt cacggacaag ggccgcatca ccgcggtcgg gcggctcaag
     1261 gacctcgaca cctcacactc gagcgtgacg atcgacgcca aggggacgac ggtcgcgccg
     1321 ggcctcatcg acagccacgt gcacccggtg ttcggcgact ggacgccgcg ccagagccag
     1381 atcggctgga tcgacagctt cctcaacggt ggcgtcacca ccatgatctc ggccggcgag
     1441 gtgcacctcc ccggccggcc gcgcgacatc gtcggggtga aggcgctcgc catcaccgcc
     1501 cagcgcgcct tcacgaactt ccggccgggc ggcgtcaagc tccacgccgg cgcgccgacg
     1561 atcgagcagg gcatggagga ggccgacttc gcggaaatgg cgaaggcagg cgtgaagctc
     1621 ctgggcgagg tcggactggg caccgtcaag gccggggccg aagcgaagaa gatggtcgcc
     1681 tgggcgagga agtatggcat ccagtcgacc atccacaccg gcggcccctc cgtgccaggc
     1741 tccggctaca tcgacgccga taccgtgctc gaagcggatg cggacgtaat cggccacatc
     1801 aacggcgggc acacggcgct gccgctgaaa cagatagagt gcctctgcgg ccagtgctcg
     1861 cgcgcgatcg aggtcgtgca caacggcaac gagtatgccg gcctccacgc cgtgaagatc
     1921 gcgcagcaat tgaagcagct cgaccgcgtc atcctcggca ccgacagtcc ggcgggctcg
     1981 ggcgtgcagc cgctcggcat cctgcgcatg atcgcgctca ttgcgagctt ctccgacctc
     2041 gcgcccgaga ccgtcgtctg tttcgcgacc ggcaacacgg cccgcacgcg cgacctcgat
     2101 tgcgggctga tcgagcccgg ccgcgcggcc gacttcgtca tcatggatcg cgcgcagcat
     2161 tccgccggtc gcgacatcct cgcctcgatc aagggcggcg atctccccgg catcggcatg
     2221 gtgatcatcg acggcgtggt gcgctgcggc cgctcgcgca atacgccgcc cgccgaccgg
     2281 ataccggagg tcatcacgca cgcccactga cggtgagcgc cgcatctcgg tccagacgat
     2341 cccacgatac cgcccccagc aacaggcgat caccatgagc actccatcga cgcacaagcc
     2401 cctcgatgcc gccgccagcg atccgaaggc gctccgctgg atgaccggat ttccacccgc
     2461 gcccgacgtc gccatcacat ttgccgacgg caccttccgc gagtttccgc agtcccgctg
     2521 ggcctacagc aattatcgcc agttcctgcc gacgaagacc gtgtggcgcg gccgtggccc
     2581 cgtgtccgag ctgaagcgag acgagcagcc gctcggcatt ctcgcgtaca cgaccatgga
     2641 cggccggcgc atcaccttcg atcaggcgct tgctgagacc tatgcagacg gcatcgccat
     2701 cctgcacagg ggccgcctcg tcttcgagcg ctatttcggg gcgctcgagc cgcacaagcc
     2761 gcacatcgcc atgtcggtga cgaagtcgtt cacgggcacg cttgcggccg cgctcgtcgc
     2821 cgaaggccgc atcgatccga aggcgcgcgt cccagagtat gtgcccgagc ttgcccagag
     2881 cggcttcggc gacgcgactg tcggcgaggt catggatatg accactgcca tcgactacgc
     2941 ggagatctac accnnnnnnn nnngacaaga actcggacgt gtgggccatg cgccgcgcca
     3001 acggcacggc cccgcccgag cccggcgcac ctcccacgtc gctcctccac tatctgacaa
     3061 cactcaagaa ggagggccgg cacggcgagg cgttcaccta ccgcacgccc aacacggacg
     3121 tgctcggctg gattgtgcgc cgcgtgacgg gccaaggcct cgccgagctc gtctcggagc
     3181 gcatctggca gcagatcggc gcggaggagg atgcccacta caccgtcgac cgcctcggca
     3241 ccgagtacgg cggcggcgga ctcaacacca ccgtgcgcga cctcgcccgc ttcgccgaaa
     3301 tgatgcgctg ccgcggtcgc ttcaacggcc gccagatcat cccggctgcc gcggtggacg
     3361 acatcgccaa gggcgctgat ccgaagaagt tcgcaccggc tggctaccag acacttcccg
     3421 gctggtccta tcatcatcag tggtgggtca gccacgacag ccacggcgtc tacatggcgc
     3481 gcggcatcca cggccaatcc atctacgtcg acccaaaggc tgagatggtg atcgcgcgct
     3541 acgcttccca tccgatggcc ggcaacgtca acaacgaccc cgtgacgctg cccgcgttcc
     3601 gcgcggtggc cctcgcgctg atggggtgac ggacagcact cagaactcga gcggcgcgtt
     3661 gtcgacgact tccttcatga cgaagaaggt gcgcgtttgg cgcacgcccg gcaggccgat
     3721 caactgttcg ccgtgcaggc ggttgaaatc ggccatgtcg cgcacgcgga tcttcaggaa
     3781 gtagtcgaaa tcgccggcga cgaggtggca gtcgaggacg acgcggagct tccgtgcggc
     3841 cgcctcgaag gccgcaaagc tgtccttcgt cgagcggtct agcacgacac cgacgaggac
     3901 gagcgtccct cgctcgacgc ggtgaggcgc gacctcggcc ctgacgccgc gaacgaaccc
     3961 atcggcgaaa agccgcgcga ggcggcgatg gcaggttgcc gggctgacat tggccgcggc
     4021 cgcaaggtcc gcattcgaga tgcgcccgtc gtcctggagg aggcgcagga gccgcctgtc
     4081 ggtgcgatcg agcagcgccg tatcggaagg ttctttcgtg agttggcgct ctttcgacct
     4141 tttcatgtca cgttctgcat ccaatacacg gtttcgatca ttttcttgac gatattcgaa
     4201 agcaccgacc ggtcaagacg ggctattctc gctccggatc cacgtggagg cgaccatgct
     4261 gaagctcgac aagttcccgc gctactcgct gacgttcggg ccgacgccca tcgagttcct
     4321 gccgcgcatg acggcagccc tcggcggcaa ggtgcagatc tatgcgaagc gcgacgactg
     4381 caactcgggc cttgccttcg gcggcaacaa gctcaggaag ctcgagtaca tcgtgccgga
     4441 cgccatcgcc tccaacgccg acacgctggt ctcgatcggc ggcgtgcagt cgaaccacac
     4501 gcgcatggtc gccgcgacgg ccgccaagat cggcatgaag tgcgtcgtca tccaggagag
     4561 ctgggtgccg cacgaggacg ccgtctacga ccgcgtcggc aacatccttc tgacccgcct
     4621 catgggtgcc gacagccgca tcgtctccga cggtttcgac atcggtatcc ggaagagctg
     4681 gggggaggcg atcgaatccg tcaagaaggc cggcggcaag ccctatgcga tcccggcggg
     4741 cgcctcggtg cacaagtacg gaggcctcgg ctacgtcggc ttcgccgagg aggtccgcgc
     4801 ccaggaaaag cagatgggcg tgaggttcga ctacatcatc gtctgcgtgg tcacgggctc
     4861 gacacaggcc ggcatgatcg tcggctttgc cgccgatgat cgcgcgagcc gggtgatcgg
     4921 cattgacgga tcgggcacgc cgaagccgct cagggcgcag gttcgccaga tcgtcgacaa
     4981 cacggctgag ctcgtggagc tcggccggaa ggtgcgcgac gacgagatcg tcatcctcga
     5041 ggactacgcc tacccggcct acggcgtgcc gagcgccgag accaacgagg cgatccgctt
     5101 tgccgcccgc accgaggcca tgatcacgga ccccgtctac gaggggaagt ccatgcaggg
     5161 catgatggac ctcgtgaaaa agggatactt cccggcagga tcgaaggtgc tctacgccca
     5221 cctcggcggc gcgccggcga tcaacggcta cagctatacc tatcgcaacg gctgaagagt
     5281 cccgcgcgcg agggcgtttt cacaggccga cgcgcgcgga gatcacttcc agctgcgctg
     5341 gatgagaccg cgctcctccg aaggagagac ggccgcgccg gcaccgagcg cgatgagcag
     5401 cgccttcagc gcatccttct cgtccgcgcc ggagggccgg aacttcccgt ccggcgtcgc
     5461 cgatgcggca acccgcacga ccccctccac gacccatccg ccggcgccat ccgcgcacgc
     5521 aatcgccgcc agctccggcg ccgtcgacgt atccttgccg tcgagctgct cgatctcgcg
     5581 gcagggccgg ccggtgcggt cgcgaaacgt ccccactacc tcgatccgcc gcgcccctgc
     5641 gccgatctgt tcgccctccc cggtcgtccg cgaatggagc aggcgtgcca gcgcgctgcc
     5701 gcttgcaacc ggtccagccg caatcacagt ggagatggag gggctgcgga agtcccgcaa
     5761 gccgaacccg acgcccgcac cgacgacgag cgcgagcgac gcggccatcg ccatcggcag
     5821 gaacatgccg cgctgagaca gacggcgtcg cccgatcggc gtgatcgtcg tcgtgtgctc
     5881 ggcgtccacg acgccgggtc gtgccgccgg actttcctgc aacaccgcga tcagacgatc
     5941 gggcaccggg ctcgatgcgg gcacctcgaa agcctgcttc aagagtgctg ctgacttctg
     6001 cagcctattc acgagctcgg cagccttcgg atcggaggcg aggtaggcct cgatctccct
     6061 gcgcttggcc tcgtcgagct cgccgtcggc gtattgcacc aacatggtct catccggcgc
     6121 tgtcatggca atgttacttt caagctctgt ctgtcacgcg gaggagcctc gcttgcccgc
     6181 ggccgcgacc catcgtctgg agggcgtttg gcttcaaccg agcggagccg gccgaacctt
     6241 tgccacaagc gatccacgcc gtggcgagtc cgccgctgaa aggcaccgac accgcgccat
     6301 gcatgatcat tccacgagag caccgtcgcc accaatcgct ctggcaccgg atctcgcagg
     6361 gaccggtcga acgcccagcc gatcagcacg gccgatcgcg tcaaccgcag cacctcctcg
     6421 cgcgcctgcg aggagcgatc gatgaggagc tgcagtgccg cccgctcttc ggaggcgagt
     6481 tcgccatcag cgaaggcaac gatccaagta tcggttgaat ggttcatggc aacaggatct
     6541 cctcaggccg ggtcgatcgc atcggccaac ctccgccgag cccgcgacag gcggctcatg
     6601 acggtgccga tcgggatctc cagggtgtcg gccgcctcct gataggacag cccctctatc
     6661 gccaccaatg acagcacgat gcgctgctcc tcgggcaact ttgccatctc gtcgcgcacg
     6721 atcgcgaggg ccgcacgcgc ctccgtcctc tcgtggatgc gcgcatcgtc ggcgacgttc
     6781 ccgagtgcct catcgtcgct ggcggtcggg cggcgggccc gcgctcgacc ctggtcgacg
     6841 aacgtggtct gcacgatgcg aaacatccag ctgtcgaggc gcgtgccagg tgtgaactga
     6901 tggaggcgcg acaaggctct gaggcatgcg gcttggacga tatcgtcagc ctgctcgggc
     6961 gagcctgaga gcgcataggc gaacctcctg agacgcggca gcagagtgat gatctcctgc
     7021 cgcagggtcg actccgtggt cgatgcttga atggccaaac gcttgctcct cagcgtcgca
     7081 actcatcgac agccggcaaa cgcagtgaac tggatgaggc cccagccgta gaccgggtcc
     7141 cggcccggtg cgccgagatc acgcgccgat cccgccagac gctccgtcag tgaggccagg
     7201 gtcggccttg ccgggtcggt cgcaacaccc atggcaaagg ctgcggcgac gaacggcgag
     7261 gcgaacgagg tgccatccac ccgctgcagc cggccggcag gcgctgccgt gagaagaccg
     7321 acgcccggag ccgcgaacgc gatgtgcttc cctcgcgtcg acagccgcga cgagcgcatg
     7381 cggtcgtcga tcgcgccgac cgcgacgaca ccgtcgtagc gcgcgggata gccctggctc
     7441 gagccgctcg ccggcgggcc agacgcggcc acgatcacga cgccgcgctc gatcgtcttc
     7501 ctgatcgctg ccagaagcac ggcgttgtcc ggcccgctga ggctcatatt gatcgtcacg
     7561 acgtccactt cgacgagcca gtcgagtgcc gcaaccaggg caaatacgtc cgccgccatc
     7621 tcggcgccct cgctgtggaa ggcgtcggct gcgaacagct ggctgtcggg tacgagcccg
     7681 acgacctcgg agttgcgagc gccgacaagc aggctcgcga cgccggtacc gtgatccgcg
     7741 ctcgacggct tgcggccgct cgcacgatgg tccttgaccg tgatcctcgc cgtagtgagt
     7801 gcagggtggc tctggtcgac gcccgtgtcg atcatgccaa ttcgcactcg cgcgctgcac
     7861 ttgccgatgc catcgctcca cgccgtcagc gtgaatgcgc tgcaggtctg accacagcct
     7921 tcaccggccg ctttgtagga gccgcggttg cgctggtaga ggtcattgtt ggcgatggtg
     7981 ctgccgggga ccgcggcggc gatccgccga cgggcagtgt cgcgacccat tccggcggga
     8041 gcgcgcaccc gcagaactga cgagccgagc aggcgattat cgcgctgatc gaggagctcg
     8101 aagccggccg acctcacggc gttcgaagca tcggccgtcg gtgcggcgac gacgagctcg
     8161 tgacggacgg gtggcgccgc cggccgcgcc tccttgctgg gcgtcgactt ggagcggcgc
     8221 gcgcgcgtct gtttgcaagc gcaaaagatg acgaggcaga cgcacttcga ggcgcgcgtg
     8281 ccgccacgag ccggcgccat ccagtcgccg ccggagccag agtcgccggc cgatccgccg
     8341 cccggccccg ctccaccgcc atcaccatca tcggcgagcg ttggcgtcac tccgaacggc
     8401 gacatcgtct gtaggctcga ggccccgaag attaggagcg caactagtcc cagttgcatc
     8461 aggcgcctgg accacgacca tgacatgagt tgcctccctc tgaggaattc acccggcgat
     8521 catttcagat ccgacacgac ggactggtcg aatccgcatc agtgttccag ccacaaaaat
     8581 cgtcagagcc gatgtatttc atcgtaactg acgataaatg cgcccggaac ggacttagtc
     8641 caggaaccaa acgatttatt gagcgagaac gtattgcaca gaccaatatt ccagagaaat
     8701 gcaagtttgt gtccgacaat ccaccgacaa ctcccgccgg tacggccgca tccgagacaa
     8761 gggccgtccg cagccacgat cacccgccac cggcagctgc gcaaacgcgg actggcgcgt
     8821 gcgagctgcc gcgaggccac cagactgccg gtcggaagcc cgcacgatgc gccgcctctg
     8881 gaatagccga gtgatgccgg cgttcttccg ccaaacgcga caagtttcca tccgactgcc
     8941 gatgcctgca ccgcagtcct gcgaggcgaa ggcgcacgcg cgggagtccc cgctctgcca
     9001 gcacgtccag ttccgacagt gtcgacgagg agccgccaat ggaccggttg acgaaaatcc
     9061 tgctcaccgc cgcggtcacg gcgctcgtca ccgccggcct cgcgatgatc gccgtcacca
     9121 ctctggacat gccactcggt gccggacgca gcagcgaggc atgcgatgcg agcttcgacg
     9181 atgatcagcg caagtgcccg aagcgctgag caagcaaacg gacgttctgc gcagtcgcgc
     9241 cactttgtct gatatctgaa gttccgagag gtgtgcccgt cccctccccc cgtgattgcc
     9301 gccggcccgg ccggggccat cgctacacct gacggccacc caggccgctc cggagcggct
     9361 acacccactc cctcgcaaat tcaattgccc gacgaacgcc cgacgcacga gcaggtcgcg
     9421 cagcgtgatg aaggcggccg gcacgaacac gcagcgcgac gtgccgccgg tcacctcgaa
     9481 gccctcgaag acgacgtggt tggactggcg gaactcgatc gtgttgctgc cgccggagag
     9541 gatcggacgt tgcccgttca cgcgcaggcc gcggatcgtg accggcaggg ccgcgctgcc
     9601 cgaatcgcgc aggatcacgc cgccggcata ggtcgcgttg ccgtccactt cgacgaggtc
     9661 gccgggctgc agcgtgacgc cggcgagcaa ggcctgcagg gtcggatacg ggcggctcgg
     9721 gccgaccgcg tagctggccg cgccggcatt gccgccggcg gccag
//
LOCUS       DAORNH010000168         9614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5236_length_9614_cov_2.596297, whole genome shotgun sequence.
ACCESSION   DAORNH010000168 DAORNH010000000
VERSION     DAORNH010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_9614_cov_2.596297"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PK264_15955"
     CDS             complement(<1..433)
                     /locus_tag="PK264_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRD77401.1"
                     /translation="MAAKTDHHSCDILVVGLGPAGASAAREAARHGARVLGFDRRSRP
                     GFPVQCAEFVPALMGPAAASVTGARRQAIASMSTFVEGGPPHVRDQFPGTMIDRERIY
                     DRLGWQGEEHNAGFVVGERIFHMDGTSERCNLTQHVKNATKN"
     gene            complement(435..806)
                     /locus_tag="PK264_15960"
     CDS             complement(435..806)
                     /locus_tag="PK264_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77402.1"
                     /translation="MKAPGERILYGEPQTDGSTPFWRMPTLWAAAAGAPIEEREISSL
                     GVLDEVVWFGGPKDVRPTVRKVAERASDIMRADLNFPIIMTRGGDILDGAHRIARAYL
                     EGKTHIRASVIDDWPPPDGVA"
     gene            complement(813..1235)
                     /locus_tag="PK264_15965"
     CDS             complement(813..1235)
                     /locus_tag="PK264_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77403.1"
                     /translation="MQFVCDAPPKTWFRFETQGEADTEAAAMAHAVDKYFRQAHDTAL
                     KSYVPPKSGAAFEQSIGRKDHIQKVMPLFLTLRDREGTPLVTAMLPPGGRENRSFRPI
                     IVGRANMDPYPDHGPAIAALATHFKLALDRERCFPYRH"
     gene            complement(1401..2150)
                     /locus_tag="PK264_15970"
     CDS             complement(1401..2150)
                     /locus_tag="PK264_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRD77404.1"
                     /translation="MLDRSVFLRPIAHRGLHDQGRGRIENTASAFKAAIAKGYGIECD
                     LRPARDGTPFVFHDLELKRLVEATGRISERTPAEIGKLRLRGSEDGILAYGDFLDLVG
                     GRVPLLVEIKSEWDTPDVTFLKKIADISAAHKGPLALMSFDPGLMAAVRPLAPAVPRG
                     IVSGGYTGHDWWRHRIDLKRAFRLKHLLESGPVAPSFFSYHVKSLPTPVTRFVREVLA
                     IPLFTWTVRSAVDRAITQRWADAMTFEGFEP"
     gene            complement(2155..2619)
                     /locus_tag="PK264_15975"
     CDS             complement(2155..2619)
                     /locus_tag="PK264_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD77405.1"
                     /translation="MSAIDARLKELGITLPAAAAPVANYVPCHIIGDLLFVSGQVSRT
                     PDGKVMAGKLGAGVSVADGQVAARSCALSILAQAKAALGSLDRIVQVVKLTGFVNAAP
                     TFTDHPQVVNGCSDCLVQVLGDKGRHTRSAVGMGSLPLDAAVEVEAIIQIRK"
     gene            2772..3653
                     /locus_tag="PK264_15980"
     CDS             2772..3653
                     /locus_tag="PK264_15980"
                     /inference="COORDINATES: protein motif:HMM:NF020478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HRD77406.1"
                     /translation="MQFALNVPRWPNAGHWLAGLGVAAVVLVAMVPATPRADVGSVVL
                     APHRAVYDLSLHRTGSGSSVTDVAGRMVYELVGSSCAGFTQTMRFVMQSSSNEGQDNL
                     IDMRSTSSEDAAGRTFKFATSQYRNQKRAEGTEGEATRGDPIRLELTRPEKKSTTIGA
                     SAYFPIQHSIELIRRARAGDKIFEADLYDGGDKGEKVYATVSLIGPKREGANKTLTKI
                     DGTTPLDSLPAWPVTMSYYELASAKQDATPSYEMSYLFFDNGVSRRIIIDYGEFAVRA
                     TLSKIEFLEPAKCAPAK"
     gene            complement(3830..5266)
                     /locus_tag="PK264_15985"
     CDS             complement(3830..5266)
                     /locus_tag="PK264_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD77407.1"
                     /translation="MSRQNALKFYIDGVWVDPVTPKSVDVINPATEQPVGKVAMGSAA
                     DVDKAVAAARRAFTTFSRTTKEERMALLAKIAEIYKRRFNEMGAAISEEMGAPLSFAT
                     RFQAGAGMGHFRTAHDLLKEFHFHERLGTTEVIREPIGAVGMITPWNWPANQIACKVA
                     GALAAGCTMVLKPSELAPTSALLLAEMMHEAGVPKGVFNLVNGDGPSVGAAIAAHPGL
                     DMVSFTGSTRAGVEVAIRAAPTVKRVGQELGGKSANILLEDADWSKAVTQGVEACFRN
                     AGQSCNAPTRMMVPAARMSDVAAIAKAAAEKLKVGDPKATDTQIGPVVSAAQWGKIQD
                     LIKKGIDEGATLVTGGPGRPADLNTGYYVRPTVFANVKPEMTVAREEIFGPVLSIMGY
                     KDEADAIRMANEHIYGLAAYVSGANADRVKDVAKQLRAGMVHLNMAAADSKAPFGGYR
                     QSGNGREWGRFGIEEFLEVKAVMGWNAA"
     gene            5402..8332
                     /locus_tag="PK264_15990"
     CDS             5402..8332
                     /locus_tag="PK264_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77408.1"
                     /translation="MIRTWRLCLGVLGLAAVTTGSLSAAEPKSVDLLKHGSARIEHAS
                     DALAGAATLKALTDGDLATPVPLAVSAGDAVTLTFAFAGGEVSPEAIEVSVGSAKREE
                     AAAAIDVLVSSVSPHAGYRSVRADPLKAAPGVQRFTFTPAAATFVMLKLTAPRGAPRV
                     AIAEVRLIGRLGPPQSNYKFGETPAKALDVIQSLAGIGTLDLSITPDEASLFEDARDG
                     KLDRWSFAEAALIVSGVRTKAERARYLSEIDAIEAEARRVTASEVGAHAKGGALLPFL
                     HKRTFAKGYVSKQTDVSTILSERTFNCVSSAALYNIVGRRLGLDLRAIEVPDHALSIL
                     YEGTSHADVETTTPRGFNPGRDRSVQDEFKSLTGFAYIPDSNRDQRREVGELGLLALI
                     YYNHGVGHSNAKRPREALLAYFRAMSLDPEFGSAVKNALATLANWSHDLSKYGQYEEA
                     LKVAGLGLRLAPKDATLLNNNKAVWSIWADSLIKAGRSEEALAVLLRAASAVPDGGFQ
                     RMQAWVYIHRGEQHIKAGEWQAAAALGPVALARLEGGARDEMARWVASLHGRRVQGEI
                     KAGRFAAALDLIAARITEAPREPRSAENLGYVVQELLKSVGAKEGTEAAERLIPDLLK
                     RFAGQRDVTEVAVAYFHRTIGRLENEGRYDDALAVAGRMEAALGDVKRAAEARAGIVD
                     RWALKLAKAGSFAAALDVYEKAYPTLASKSTADNNIRYIIQEWTKAAAAKGGSPTAEE
                     ILAAQTKRFAFVPGLAGASDDHVANTAIGHARAKRFSQALDVYAAAYATLKDKSKAHN
                     NILYVLQEWTKEAAATGARPSAEEILAKELQRFSFVPGIGQIAVGHVNRLVQAEVREK
                     RFEAALAVLKANAGVLPDPKKAEDIAAYVYDSWAKTHRERGDWQAVVDLYNRSLEAYP
                     GSSHLRRNAIAAWYGWSKVFMDKKDWRGAITIYENALKRFPGESLLEQNLKYCREQLA
                     KSGG"
     gene            complement(8419..9015)
                     /locus_tag="PK264_15995"
     CDS             complement(8419..9015)
                     /locus_tag="PK264_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77409.1"
                     /translation="MIEACRSLLRTALLVMLAFLIVDGSATAQPAKRKVVVMIDDGPT
                     TSENRGSPLAHAVRQVVARHLLADFEVVEEMALGLPSAPDGRRTSESWSANFKALSGK
                     ADILVRVQVHTIVEQVPFAGKIPQYRATFRLSLEGIAVESGAVLNIRLVGPFELSSLP
                     ESCKDGSCLVNLIPRESVFSANDFARDLAKKGRPSNAK"
     gene            9178..>9614
                     /locus_tag="PK264_16000"
     CDS             9178..>9614
                     /locus_tag="PK264_16000"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HRD77410.1"
                     /translation="MSDPFVLGWEEWASLPDLGLPAIKVKVDTGARTSALHATGMRVE
                     GPADARTLSFLVHPVRRRAEPAIACRAPLVGERVVTSSNGESERRYVIATRIRFGERS
                     WPIEITLTNRESMSYRMLLGRNALAAGMLVDPLRGLLQPRLSYR"
BASE COUNT         1630 a         3177 c         3228 g         1579 t
ORIGIN      
        1 ggttcttcgt cgcgttcttg acatgctggg ttaggttgca acgctcggat gtcccatcca
       61 tgtggaagat tcgctccccc acgacgaacc cggcgttatg ctcctcccct tgccacccga
      121 gccgatcata tatgcgctcg cggtcgatca tggtgcccgg gaattggtcg cgaacgtgcg
      181 gcggaccgcc ctcgacgaac gtcgacatcg aggcgatcgc ctgccggcgc gctccggtga
      241 cgctcgctgc cgccgggccc atcagtgccg gcacgaactc ggcgcattgg acggggaagc
      301 ccggtcggga acggcgatcg aaaccgagca cacgggcgcc gtgccgggcc gcttcgcgcg
      361 cggcactggc gccagcagga ccgagcccga caaccaagat gtcacacgaa tgatgatccg
      421 tcttcgcggc catctcaggc gacaccgtcc ggcggcggcc agtcgtcgat cacggacgct
      481 ctgatatgcg ttttgccctc gaggtaggcg cgcgcgatcc gatgggcacc atcgagaatg
      541 tcgccacctc gggtcatgat gatcggaaaa ttcagatcgg cgcgcatgat atcggaggcc
      601 cgttcggcca ccttgcgcac cgtcggtcgc acatccttcg ggccgccgaa ccagacgacc
      661 tcgtcgagga cgccgagcga gctaatctca cgttcctcga tgggtgcccc ggccgcggcc
      721 gcccagagtg tcggcatccg ccagaagggc gtcgagccat cggtttgcgg ctctccgtac
      781 agtattcgct cgccgggagc tttcatgtcg tctcagtgcc ggtaggggaa gcagcgctcg
      841 cgatcgagag cgagcttgaa gtgggtcgcg agcgcggcga tggcaggacc atgatcgggg
      901 taggggtcca tgttggcgcg gccgacgatg atcgggcgga acgagcggtt ctcgcggcct
      961 cccggcggca gcatggcggt gacgaggggc gtgccctcgc ggtcgcgcag cgtgaggaac
     1021 aggggcatga ccttctggat atgatccttg cggccgatgc tctgctcgaa ggcggcgcct
     1081 gatttcggcg gcacatagct cttcaaggca gtgtcgtggg cttgccggaa gtacttgtcg
     1141 acggcatgcg ccatcgcggc tgcctcggtg tcggcttcgc cttgcgtctc gaagcggaac
     1201 caggtcttgg gcggggcgtc gcatacgaac tgcatggtgc gcggctcctc ggcggtcggc
     1261 gcgagctcag ccgtcggcgc gggatggcgc ggcgggacga tcgcgcggga aagtcttgcg
     1321 agtatatggt gcgtgccttg ccgcggcaaa ggggcgggga ggcaaaagcg caattgggct
     1381 gggcgtgtgt ggctttgcca tcagggctcg aagccttcga aggtcatggc atcggcccaa
     1441 cgctgggtga tggcgcgatc gacggcggaa cgcaccgtcc aggtgaagag ggggatcgcc
     1501 agcacctcgc gcacgaagcg ggtgacgggg gtcggcagcg acttcacgtg gtaggagaag
     1561 aagctcggcg cgacggggcc ggattcgagc aggtgcttca ggcggaaggc gcgcttcagg
     1621 tcgatgcggt ggcgccacca gtcgtgtccc gtgtagccgc ccgagacgat gccgcgcggc
     1681 accgcgggcg cgagcggccg caccgccgcc atcagccccg gatcgaacga catgagcgcg
     1741 agtggcccct tatgcgccgc cgagatgtcg gcgatcttct tcaggaaggt gacgtcgggc
     1801 gtgtcccact cgctcttgat ttcgacgagc agcggcacgc gcccgccgac gagatcgaga
     1861 aagtcgccat aggcgaggat gccatcctcg ctgccacgga ggcgcagctt gccgatctcg
     1921 gcaggcgtgc gctcgctgat acgccccgtc gcctcgacga ggcgcttcaa ttcgagatca
     1981 tggaagacga acggcgtccc gtcgcgcgcg ggtcggaggt cgcactcgat gccgtagccc
     2041 ttggcgatcg cggccttgaa cgccgaggcc gtgttctcga tgcgaccacg gccctggtca
     2101 tgcaggccac gatgagcgat cgggcgcagg aacaccgagc ggtcgagcat gcggctattt
     2161 ccggatctgg atgatcgcct cgacctcgac cgcggcatcg aggggcaggc tgcccatgcc
     2221 gaccgccgag cgtgtatggc gacccttgtc gccgagcacc tggacgaggc agtccgagca
     2281 gccgttcacc acctgcgggt gatcggtaaa ggtcggcgcc gcgttgacga agccggtgag
     2341 cttcacgacc tgcacgatgc ggtcgaggct gccgagcgcg gccttggcct gggcgaggat
     2401 gctcaatgcg cacgagcgcg ccgcaacctg gccgtcggca acggagacac ccgccccgag
     2461 cttgccggcc atgaccttgc catcgggggt ccgcgagacc tggcccgaga cgaacagcag
     2521 gtcgccgatg atgtggcagg ggacgtagtt ggcgaccggc gcggcggccg ccggcagcgt
     2581 gatgccgagt tccttcaggc gggcgtcgat ggcgctcatg tcgatttctc cctggtcgag
     2641 gcggtatcgt gcactgtggg ccaacagctc ggcgctttgc aaccgacttc gctgtcggta
     2701 cattcaggcg ctgccgcccg gcgagtccgg ctccatccgg agccccgatc ggcggcattt
     2761 cggagacacg catgcagttc gcgctgaacg tcccgaggtg gcccaatgcc gggcactggc
     2821 tcgccggcct cggcgtcgct gctgtggtgc tcgttgccat ggtgcctgcc accccgcgcg
     2881 ccgacgtcgg cagcgtggta ctcgcgccgc atcgcgccgt ctacgatctg agcctccatc
     2941 gcacgggcag cggctcgagc gtgacggatg tggccggacg catggtctac gaactcgtcg
     3001 gctcgtcgtg cgcgggcttt acgcagacca tgcgcttcgt catgcagtcg agcagcaacg
     3061 aagggcagga taatctgatc gacatgcgct cgacgagcag cgaggacgcg gccggccgaa
     3121 ccttcaagtt cgcgacgagc cagtaccgca accagaagcg cgccgaaggc accgagggcg
     3181 aggccacgcg cggcgatcca atcagattgg agctcacgcg gcccgagaag aagtcgacaa
     3241 ccatcggcgc cagcgcctat ttcccgatcc agcattcgat cgagctgatc aggcgcgcgc
     3301 gggccggcga caagatcttc gaggccgacc tctacgacgg cggcgacaaa ggcgagaagg
     3361 tctatgcgac ggtgtcgctg atcggcccga agcgtgaagg cgcgaacaag acgctcacca
     3421 agatcgacgg caccacgccg ctcgacagcc tgccggcatg gccggtgacg atgagctact
     3481 acgagctcgc ctcggcaaag caggacgcga cgccatccta cgagatgtcc tatctcttct
     3541 tcgacaacgg cgtgagccgc cgcatcatca tcgactacgg cgagttcgcc gtgcgcgcga
     3601 cactcagcaa aatcgagttc ctggagccag ccaaatgcgc gccggcgaag tagcggccat
     3661 tcgtggtcgc cggtcccgtg gcccgcgtct cagccctgcg gataaggcct gcagccggtt
     3721 tccaaaggcc cgaggccttt ggcggggctc ggggcagagc cccgaaccgt cattgagcgc
     3781 cggccgagca catggcgacg cttcgcaagc gagggcccgg tccggcgctt caggccgcgt
     3841 tccagcccat cacagccttc acctcgagga attcctcgat gccgaagcgg ccccactcgc
     3901 ggccattccc cgactggcgg tagccgccga acggcgcctt gctgtcggcc gccgccatgt
     3961 tgaggtgcac catgccggcg cggagctgct tcgccacgtc cttgacgcga tcggcgttgg
     4021 cgccgctgac gtatgccgcc agcccgtaga tgtgctcgtt cgccatgcgg atggcatccg
     4081 cctcatcctt gtagcccatg atcgagagca ccgggccgaa gatctcctcg cgggcgacgg
     4141 tcatctccgg cttcacgttt gcgaacacgg tcgggcggac gtagtacccg gtattgaggt
     4201 ctgccggccg ccccggaccg cccgtcacga gcgtcgcgcc ctcgtcgatg cccttcttga
     4261 tgagatcctg gatcttgccc cactgcgccg ccgagacgac cgggccgatc tgcgtgtccg
     4321 tggccttcgg gtcgccgacc ttcagctttt cggcggcggc cttggcgatg gctgcaacgt
     4381 cgctcatgcg ggccgcagga accatcatgc gggtcggcgc gttgcacgat tggccggcat
     4441 tgcggaagca ggcctcgacc ccctgcgtca cagccttcga ccaatcggcg tcctcgagca
     4501 ggatgttggc cgacttgccg ccgagttcct ggccgacgcg cttcacggtc ggcgcggcgc
     4561 ggatcgcgac ctcgacaccg gcccgcgtcg agccggtgaa cgacaccatg tcgaggccgg
     4621 ggtgcgcggc gatggcggcg ccgaccgacg gcccgtcacc gttgacgagg ttgaacacgc
     4681 ccttcggcac gccggcctcg tgcatcatct cggcgagcag cagcgcggag gtcggcgcga
     4741 gctccgacgg cttcagcacc atcgtgcagc ctgcggcgag cgcgccggcg accttgcagg
     4801 cgatctggtt cgccggccag ttccagggcg tgatcatgcc gacggcgccg atcggctcgc
     4861 ggatcacctc ggtcgtgccg aggcgctcgt ggaaatgaaa ctccttcagg aggtcgtgcg
     4921 cggtgcggaa gtgacccatg ccggcgccgg cctggaagcg cgtcgcgaag gagagcggcg
     4981 cgcccatctc ctcggagatc gcggcaccca tctcgttgaa gcggcgcttg tagatctcgg
     5041 cgatcttggc gagcagcgcc atgcgctctt ccttggtcgt ccgggagaac gtcgtgaagg
     5101 cacgccgcgc cgccgcgacg gccttgtcga cgtcggcggc ggagcccatg gcgaccttgc
     5161 cgaccggctg ctcggtcgcc gggttgatca catcgacgga cttcggcgtc acgggatcga
     5221 cccagacgcc atcgatatag aacttcagcg cgttctggcg gctcatggca gtgctcctcg
     5281 attgttgtgc gcggtctagc aggggagggg ctgccctgtc gacacgctgc tgactgggct
     5341 ggtcgggcgc gctatgatgc ggatggacat tagatttgct cgtttcgacg gagctccggc
     5401 aatgatccgc acctggcgcc tctgtcttgg tgtgctcggc cttgccgccg taacgaccgg
     5461 cagcctgtcg gccgctgaac ccaaatcggt cgacctcctg aagcatggaa gcgcacgcat
     5521 cgagcatgcg agcgatgcgt tagccggagc agcgacgctc aaggccctga ccgacggcga
     5581 tcttgcgacg ccggttccgc tcgcggtctc ggccggagat gctgtcacgc tcactttcgc
     5641 cttcgcaggc ggcgaggtgt cgccggaggc gatcgaggtg agcgtcggta gtgccaagcg
     5701 cgaggaggcg gccgccgcga tcgacgtgct cgtctcgagt gtgtcgccgc atgcgggcta
     5761 tcgctcggtg agggctgatc cgctgaaggc cgcccccggt gtccagaggt tcacgttcac
     5821 gccagcggcc gcaacgttcg tgatgctgaa gctcacggcg ccccgtggcg ccccgcgcgt
     5881 cgcaatcgcg gaggtgcgtc tcatcggccg gctcgggcct ccgcagtcca actacaagtt
     5941 cggggagacg ccggcaaagg cgctcgacgt gatccagagc ctcgccggca tcggcacgct
     6001 cgacctctcc atcaccccgg acgaagcgag cctcttcgag gacgcgcgcg acggcaagct
     6061 cgaccggtgg agcttcgccg aggcagcact catcgtctcg ggcgtgcgca ccaaggccga
     6121 gcgcgcgcgc tatctctcag agatcgacgc catcgaggcg gaggcgcggc gcgtgacggc
     6181 atcggaggtg ggcgcgcacg ccaaaggcgg cgccctgctg ccgttcctcc acaagcgcac
     6241 ctttgccaag ggctatgtct cgaagcagac cgacgtctcc acgatcctct ccgagcgcac
     6301 gttcaactgc gtctcctcgg cggccctcta caacatcgtc ggccggcgcc ttgggctcga
     6361 cttgcgcgcc atcgaggtgc cggatcacgc cttgtcgatc ctctacgagg ggacaagcca
     6421 tgccgacgtc gagacgacga cgccgcgcgg tttcaatccc ggccgcgaca ggtcggtgca
     6481 ggacgagttc aagtcgctga ccggcttcgc ctacataccc gacagcaacc gcgatcagcg
     6541 ccgcgaggtg ggcgagctcg ggcttctggc cctcatctac tacaaccacg gcgtcggcca
     6601 ctcgaatgcg aagcgaccgc gcgaggcgct gctcgcctac ttccgcgcca tgagcctcga
     6661 tccggagttc gggtccgccg tcaagaatgc gctcgcgacg ctcgccaact ggagccacga
     6721 tctctcgaag tacgggcagt acgaggaggc cctgaaggtc gcgggcctcg gcctgcgtct
     6781 cgcgccgaag gacgccacac tcctcaacaa caacaaggcc gtgtggtcga tctgggcgga
     6841 tagcctgatc aaggcggggc ggagcgagga ggcgctcgcg gtgctcttgc gcgcggcgag
     6901 cgccgtgccc gacggcggct tccagcgcat gcaggcgtgg gtctacatcc atcggggcga
     6961 gcagcacatc aaggccggcg agtggcaggc ggcggcggcg cttggaccgg ttgcgctcgc
     7021 ccggctcgag ggcggagcgc gcgacgagat ggcccgctgg gtcgcatcgc ttcacggacg
     7081 ccgcgtccag ggcgagatca aagccggtcg gttcgccgcg gcgctcgacc tcatcgcggc
     7141 ccgcatcact gaagccccgc gcgagccgcg cagcgccgag aacctcggct acgtcgtgca
     7201 ggagctcctg aagagcgtcg gcgccaagga gggcaccgag gctgcggaac gcctgatccc
     7261 ggacctgctc aagcgctttg ccggccagcg cgacgtgaca gaggttgccg tcgcctactt
     7321 ccaccggacg atcggccgcc tcgagaacga gggacgctat gacgacgcac tcgccgttgc
     7381 cggccgcatg gaggccgcac tcggcgacgt gaagcgcgcg gccgaggcac gcgcaggcat
     7441 cgtcgatcgc tgggcgctga agctcgccaa ggcggggagc ttcgctgcag cactcgacgt
     7501 ctacgagaag gcctacccga cactcgcgag caagagcacc gccgacaaca acattcgcta
     7561 catcatccag gaatggacga aggcggcggc ggccaagggc ggctcgccca cggccgagga
     7621 gatcctcgcg gcgcagacga agcgcttcgc attcgtgcca ggccttgccg gcgcctcgga
     7681 cgaccatgtc gccaacacgg cgatcgggca cgctcgcgcc aagcgatttt cgcaggctct
     7741 ggacgtctat gcagcggcgt atgccacgct caaggacaag agcaaggcgc acaacaacat
     7801 cctctatgtc ctccaggaat ggacgaagga ggcggcagcg acaggtgcgc ggccatcggc
     7861 cgaggaaata ctcgccaagg aactgcaacg cttcagcttc gtgcccggca tcggccagat
     7921 cgcggtcggc cacgtcaacc ggctggttca agccgaggtg cgcgaaaagc gcttcgaggc
     7981 ggcgctggca gttctcaagg caaatgcggg tgttttgccc gatcccaaga aggcagagga
     8041 tatcgctgcc tacgtctacg acagctgggc caagacacat cgcgagcgcg gcgactggca
     8101 ggcggtcgtc gatctctaca atcgctcact cgaggcctat cccggcagca gccacctcag
     8161 aagaaacgcc atcgccgcgt ggtacggctg gtcgaaggtg ttcatggaca agaaggactg
     8221 gcgcggcgcg attacgatct acgagaacgc gctcaagcgg ttccccggcg aaagcctcct
     8281 cgagcagaac ctcaaatact gccgcgaaca actcgccaag agcggcggtt agccgcgcgg
     8341 cgcccgcgcc ttgcgctacc cactcatcac cacgaccggc acgggcgccg ctgcgaattt
     8401 cttcgtccgg agcagttgtc acttcgcatt actcgggcgt ccttttttgg ccaaatcacg
     8461 tgcgaagtca ttagcagaaa agacgctttc gcgagggatc agattgacga gacaactacc
     8521 atctttgcag ctttcgggca gtgaggatag ctcgaatgga cccacgagac ggatgttcag
     8581 gactgcgccg gattcaacgg caatcccttc caatgacagt cgaaatgtcg ctcgatattg
     8641 tggaattttt cccgcaaacg gcacctgctc gactatcgtg tggacctgaa ctcgaacgag
     8701 aatatcggcc ttcccactca gggccttgaa gttggcggac cacgattcgc ttgtccgccg
     8761 tccgtcgggg gcgctcggca ggccgagtgc catctcctcg acgacctcga agtctgccag
     8821 caggtgccgt gccacgacct gccgcacggc atgagccaat ggacttcctc ggttctcgct
     8881 tgtcgttggc ccatcgtcga tcatgacgac gaccttgcgt ttggccggct gcgccgtcgc
     8941 gctgccgtca actatcaaga acgccagcat cacgaggagg gcggttcgca gtaaagagcg
     9001 gcatgcttca atcatcgtct gacatccgta tcgcaattcg aaccggtgac acgcctttga
     9061 atattgcatc gaccagtcgc gtattgtcac ttcgggtcgc aaccgaggtc ggtcacactt
     9121 gcgcattggc gccgaagcgg ctacccggct cctcgtgcat caaagcggag gcgcggcgtg
     9181 tcagatccgt tcgtgctcgg ctgggaggag tgggcgtcgc tccccgatct cggacttcca
     9241 gccatcaagg tgaaggtcga cacgggcgcg cgcacgtcgg cgctccatgc gaccggtatg
     9301 cgcgtcgagg gaccggcaga cgcgcgcacg ctgagtttcc tcgtgcatcc cgtgcggcgg
     9361 cgcgcggagc cggccattgc ttgccgggcg ccgctcgtcg gagagcgggt cgtgacgagc
     9421 tccaatggtg agagcgagcg gcgctacgtg attgcgacac gcatccggtt cggcgagcgg
     9481 tcctggccga tcgagatcac gctcaccaac cgcgagagca tgtcgtatcg catgctgctc
     9541 ggccgcaacg cgctcgcggc cggcatgctc gtcgatccgc tccgcggcct cctgcagcca
     9601 cgcctcagct accg
//
LOCUS       DAORNH010000169         9597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5255_length_9597_cov_2.890589, whole genome shotgun sequence.
ACCESSION   DAORNH010000169 DAORNH010000000
VERSION     DAORNH010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5255_length_9597_cov_2.890589"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1498)
                     /gene="mdoH"
                     /locus_tag="PK264_16005"
     CDS             complement(<1..1498)
                     /gene="mdoH"
                     /locus_tag="PK264_16005"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HRD77411.1"
                     /translation="MSNTSDTAGSPAPISRPDEPSLTTPTGLQATADLAVRRRVVIVL
                     NVLTWLVLMAVAARILGSGGWTWLDILMFVCFALGTPWTVLGFWNAIIGLWLLHGHGD
                     GLKAVAPFAAAGDVPTPLTVKTAVFMTLRNEDPERAIKRLETVKASLDRTGEGGAFSY
                     FLLSDTNDPAVAAREEALADAWKARDKDGARIAYRRRSVNTGYKAGNVRDFIDRHGAD
                     FELMLPLDADSVMTGPSIVRLVRMMQAHPRLGILQSLVVGMPSESAFARIFQFGMRHG
                     MRSYTFGQAWWVGDCGPYWGHNALVRIKPFRDHCELPILPGGPPLGGHVLSHDQVEAT
                     FMRRAGYEVRVLPVEEGSYEENPPTMLDFAKRDVRWCQGNMQYVKLLDTPGLLPMSRF
                     QLVWAILMFVGLPAWTLMIAALPLVALQAQAVPDFPTYLAKGLYITFFLMYLMPKIAG
                     LLDAVLTKGGVARYGGALRFTASAAMELVFSFLQGAVSTIRTTIFMIGL"
     gene            1562..2605
                     /locus_tag="PK264_16010"
     CDS             1562..2605
                     /locus_tag="PK264_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HRD77412.1"
                     /translation="MQNESKRDDAAAPRRARARIEVARAIWYVKPGVAEIRSARLTPP
                     GPGEARVRMLYSGISRGTERLVALGEVGESERTRMRAPLQDGEFPFPVKYGYCAVGRI
                     EAGPAERLGETVFCLHPHQDVFNAPLGMLAQVPAGIPAKRATLAANMETALNALWDSG
                     AGPGDRIVVVGAGVVGLMVASLAARLPGADVMVVDVAAERRVLVERLGAAFLEAPRAA
                     ELSGSADVVFHTSVTAGGLATAISTAGREGTIVELSWYGDKAVAAPLGGAFHSQRLRL
                     ISSQVGQVSPGRRPRWDYARRMGKALELLADARLDALVAEELAFDAAPRELPAVLAGT
                     MGGLAPVIAYGTP"
     gene            complement(2648..3340)
                     /locus_tag="PK264_16015"
     CDS             complement(2648..3340)
                     /locus_tag="PK264_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77413.1"
                     /translation="MVIALVRPHIEKSRSPRGLWVPFPLGRPLGEPEDPSFQRRVLLS
                     ALRLLERAEGPVILEDFADDAPSMRDNADWAPDPVLMRLPMSAPSDAAGWASALRAEM
                     NEIQPHWQAATHRFGRTTVGVSKLEPSRWPDYLAAWLSGTLPASPVAGLSAAVAMRFI
                     ADDLKAMYSEAVQAHGPQPAAGQVQSWFWRSTMAGALLRAIRGQSLTSADSGFNTAGS
                     RFVVPGPFVNPA"
     gene            complement(3421..3882)
                     /locus_tag="PK264_16020"
     CDS             complement(3421..3882)
                     /locus_tag="PK264_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HRD77414.1"
                     /translation="MARIEDIYQPTRDQVAGLAIAEPTTSPWVAPKPLFRSTIALVTS
                     AALHPRSTPPFAAGSAEFRKLPASTPASDLVMSHISINYDRGGFQRDINTVYPVDRMG
                     ELVAEGIVGALAPNGYSVMGSTDPATMSATADAMAADMREAKVEAALFLPV"
     gene            complement(3992..>4192)
                     /locus_tag="PK264_16025"
     CDS             complement(3992..>4192)
                     /locus_tag="PK264_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77415.1"
                     /translation="EPKPENGEHAHEFAVRGLVTAGEFIISRNGTPRSYTSGQTFEVA
                     AGEMHTEAVGPQGTDITTGRKY"
     assembly_gap    4193..4202
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4470..4721
                     /locus_tag="PK264_16030"
     CDS             4470..4721
                     /locus_tag="PK264_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HRD77416.1"
                     /translation="MAGAAVREGAMSKKISKTKTMADLEANDCRWPIGDPREANFHFC
                     GAQKVLGRPYCMEHWTLSFDATKTRRPSSAPMLPIRRAA"
     gene            4877..>5057
                     /locus_tag="PK264_16035"
     CDS             4877..>5057
                     /locus_tag="PK264_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRD77417.1"
                     /translation="MSDTYEVLAVKYGTHANRPRHLNFMNADDHASLQPIDYFVWVVR
                     NAHHTVVVDTGFTHAA"
     assembly_gap    5058..5072
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5073..5665
                     /locus_tag="PK264_16040"
     CDS             <5073..5665
                     /locus_tag="PK264_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HRD77418.1"
                     /translation="MEPIEALKGIGVDAGKVPHVVVTHLHYDHAGTLEHFPGARFHLQ
                     EAEMAYATGRCMCDEAMRLPFTADHVCQMVQRVYSGRVQFHEGDGEVVPGVTVHKCPG
                     HSQGLQSVRVKTDNGYVVLASDAAHLYENIEKRKPFSITVNMEQTLRTYDKLAALGGG
                     LKNVVPGHDPQVLKIYPAWKPETAGRVVRLDKGRIA"
     gene            complement(5986..6465)
                     /locus_tag="PK264_16045"
     CDS             complement(5986..6465)
                     /locus_tag="PK264_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77419.1"
                     /translation="MKKNNVTGLFGGLLALALGVFLVLPPAAAESYVRVENRWKPNHF
                     VHVERGRIEAGPIEAGWHSAMWVIEPVPGVPNHVRLRNRWKGNHYIHVEKAAVQAGPI
                     EPGWHSAMWVIEPVPGLPNFVRLRNRWKADHYLHVETGAIQAGPILPGWHSAMWQIR"
     gene            6936..7685
                     /locus_tag="PK264_16050"
     CDS             6936..7685
                     /locus_tag="PK264_16050"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HRD77420.1"
                     /translation="MSEQSKNFPEFYVTAPHPCPYLPGRAERKLFTHLTHDKPAFLVD
                     NLLKGGFRRSQNIAYMPYCEGCQACVSVRVIVDEFVPGRTFRRTMLGNRDLEISRIEP
                     VPSAEQYGLFRSYIDARHGEGGMADMTVLDYAMMVEDSIIETFVTEYRLRPRSPHSSR
                     PSTWPLLGVALCDRLSDGISMVYSFYDTEAQDRGLGTFMILDHIAYARRLGLPYLYLG
                     YWIEGSRKMAYKRRFMPQEQLTGGGWTRVEA"
     gene            7898..8299
                     /gene="rsfS"
                     /locus_tag="PK264_16055"
     CDS             7898..8299
                     /gene="rsfS"
                     /locus_tag="PK264_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRD77421.1"
                     /translation="MRATAGASRKRSVPAPKTKGSDALLGLVTTCLDDAKADNIVTID
                     LAGKSSLGDYMVIASGNSDRHVGAIADQLQRKLKEAGRVRVRVEGQDHCDWVLVDAGD
                     VIVHLFRPEVRDFYNLEKMWSAERAGETTQH"
     gene            8318..8797
                     /gene="rlmH"
                     /locus_tag="PK264_16060"
     CDS             8318..8797
                     /gene="rlmH"
                     /locus_tag="PK264_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRD77422.1"
                     /translation="MRLLLVAIGRLKDGPERALFERYWTRLAAMSRPLGITAIGFKEL
                     AESPARDATTRKADEGRRLIETAAGLVTIVLDERGKAMSSEAFASRLQSMAGSGGSGL
                     AILVGGPDGHSPEVLAGARLKLSFGAMTFPHGLARILIAEQLYRAATIAAGHPYHRS"
     gene            8817..>9597
                     /locus_tag="PK264_16065"
     CDS             8817..>9597
                     /locus_tag="PK264_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77423.1"
                     /translation="MYRTLDAAKLIVSLDTLGRRIGERFPDASLRQVGTDLADIAREH
                     SERARQISGANWPLRAFVAIVLAGGLWLIVRIVGLMDLSKTAADNVYTVLQGVEATLN
                     IVVLMGGALFFLITVEDRLKRRRALMAIHELRSIVHVIDMHQLTKDPSGLLQNPGQGD
                     TPSSPRRTYTPFELTRYLDYCSEMLSLAAKVAALYAQSYPDPVVTSSVNELEQLTANL
                     SQKIWQKISIVEQMIANMPRASVPSVKPAATLKPDGTKAAPV"
BASE COUNT         1660 a         3092 c         3166 g         1654 t
ORIGIN      
        1 cgagtccgat catgaaaatg gtggtgcgga tcgtcgaaac ggcgccttgg aggaatgaaa
       61 acacgagctc catcgcggca ctcgccgtga agcggagcgc gccgccgtag cgcgcgacgc
      121 cgccctttgt gaggaccgca tcgaggaggc ccgcgatctt cggcatgagg tacatgagga
      181 agaaggtgat atagaggccc ttggcgagat acgtcgggaa gtccggaacg gcctgcgcct
      241 ggagagccac gagcgggaga gcggcgatca tcagcgtcca ggcgggcagc ccgacgaaca
      301 tcaagatcgc ccagacgagc tggaagcggc tcatggggag gagccccggc gtgtcgagca
      361 gcttcacgta ctgcatgttg ccctggcacc accggacatc gcgcttggcg aagtcgagca
      421 tggtcggcgg gttctcctca tagctcccct cctcgacggg caagacgcgc acctcgtagc
      481 cggcgcggcg catgaacgtc gcctcgactt ggtcgtgcga gagcacatga ccgccaagcg
      541 ggggaccgcc ggggaggatc ggcaactcgc agtggtcgcg gaacggcttg atcctgacga
      601 gtgcattgtg accccagtag gggccgcaat cccctaccca ccaggcctgc ccgaaggtgt
      661 aggagcgcat gccgtgccgc atgccgaact ggaagatgcg ggcgaacgcg ctctcggacg
      721 gcatgccgac gacgaggctc tgcagaatgc cgaggcgggg atgcgcctgc atcatgcgca
      781 cgagacgcac gatcgagggc ccggtcatga cgctgtcggc gtcgagtggg agcatcagct
      841 cgaagtcggc gccgtggcgg tcgatgaagt cgcgcacgtt gccggccttg tagccggtgt
      901 tgaccgagcg gcggcggtag gcgatgcggg ctccatcctt gtcgcgggcc ttccacgcgt
      961 cggcgagagc ctcctcgcgg gcggcgactg cgggatcgtt ggtgtctgag agcaggaaat
     1021 agctgaaggc gccgccctcg cccgtccgat cgaggctcgc cttcaccgtc tcgagccgct
     1081 tgatggcgcg ctcggggtcc tcgttcctga gcgtcatgaa gacggcggtc ttgaccgtga
     1141 gcggcgtcgg cacatcaccg gcagccgcga agggggcgac ggccttgagc ccgtcgccgt
     1201 ggccgtgcag cagccagagg ccgatgatcg cgttccagaa gccgagcacg gtccagggcg
     1261 tgcctagcgc gaagcagacg aacatcagga tgtcgagcca ggtccagccg ccgctgccaa
     1321 ggatgcgggc ggcgaccgcc atcagcacga gccaggtcag cacgttgagg acgatgacca
     1381 cgcggcggcg gacggcgagg tcggccgttg cctggagccc tgtcggcgtc gtaaggctcg
     1441 gctcgtccgg ccgcgagatc ggggccggcg agccggcggt atctgatgtg ttgctcatgg
     1501 atgtccggtc ttgtggatgc ccggtcctgg tcggtcgcag tctcggagcg tgaagtcagc
     1561 catgcagaac gagtcgaagc gcgatgacgc tgccgcgccc cgccgcgcgc gcgcccgcat
     1621 cgaggtagcg cgggcgatct ggtacgtaaa gccgggggtt gcggagatcc gctcggcacg
     1681 gttgacgccg cccgggccag gcgaggcacg ggtcaggatg ctctacagcg gaatcagtcg
     1741 cggcacggag cgcctcgtgg ctctggggga ggtcggggaa agcgagagga cgcgcatgcg
     1801 tgcgcccctt caggacggcg agttcccttt tcccgtcaaa tatggctatt gcgcggtcgg
     1861 ccgcatcgag gcggggccag cggagcgctt gggcgagacg gtcttctgcc tccacccgca
     1921 ccaggacgtg ttcaacgcgc cgctcggcat gctggcgcag gttccggctg gtataccggc
     1981 gaagcgcgcg acactcgcgg caaacatgga gacggccctc aacgcgctct gggattcggg
     2041 cgccgggccg ggcgacagaa tcgtcgtggt cggcgcgggt gtcgtcgggc tgatggtcgc
     2101 ctcgctcgcc gcccggctcc cgggtgccga cgtgatggtg gtcgacgtcg cggctgagcg
     2161 cagggtgctc gtcgagcggc ttggggcggc ctttctcgag gcgcctcgcg ccgccgaact
     2221 ttcgggctcg gctgatgtcg tctttcatac gagcgttacc gcgggcgggc tcgcgacggc
     2281 aatttccacc gcgggacgcg agggtacgat cgtcgaactc tcctggtacg gcgacaaagc
     2341 ggtggcagcc ccgctcggtg gcgccttcca cagccagcgc ctgcggctca tctcctcgca
     2401 ggtcgggcaa gtctcgcccg gccggcgccc gcgctgggac tacgcgcggc gcatgggtaa
     2461 ggcactcgag ctgctcgccg atgcgcgcct tgatgcgctc gtcgccgaag agctcgcttt
     2521 cgacgcggcg ccgcgcgagc tgccggccgt cctggctgga accatgggtg gcctcgcccc
     2581 cgtcatcgcc tatggcacgc cgtaacgtga accatcgcgg ctgtcggatg cctggcgacc
     2641 cacacgctca agccggattg acgaatgggc ccggaaccac gaagcgcgag cctgccgtgt
     2701 tgaagccgct atccgcgctc gtgagtgatt ggcctctgat ggcgcggagc aaggcaccgg
     2761 ccatcgtcga tctccagaac caggactgga cctgtcctgc tgccggctgt ggtccgtgag
     2821 cctgaaccgc ctcgctgtac atggccttca gatcgtcggc gatgaaccgc attgcgacgg
     2881 cagccgacag accagcaact gggctcgctg gcaatgtccc tgaaagccag gccgcgagat
     2941 aatcgggcca gcgcgatggc tcgagcttgc tcacgccgac cgtcgtccgc ccgaagcggt
     3001 gcgtggccgc ctgccaatgt ggctgaattt cattcatctc ggcacgcaac gctgaggccc
     3061 agcccgcggc gtctgacggc gcactcatcg ggagacgcat gagcactgga tcgggcgccc
     3121 agtctgcatt gtcgcgcatg ctcggtgcgt cgtcggcgaa gtcctcgagg atgacaggac
     3181 cttcggcgcg ttcgagcagt cggagcgcgg agaggagaac gcgtcgctgg aatgacggat
     3241 cctcaggctc gccgagcggc cggccgagcg ggaacgggac ccagaggcca cgcggcgagc
     3301 gcgacttctc gatgtgcggg cggacgaggg caatcaccac cgtggcgatg ccgcgacggt
     3361 gggcgcgccg cgcgattcga tcatgtgggc gagcgcgctc acggcgcgcg tgcagaacgg
     3421 tcagacgggt aagaagagcg cggcctcgac tttggcctcc cgcatgtcgg cagccatcgc
     3481 gtcagccgtc gccgacatgg ttgccggatc ggtagagccc atcaccgagt agccgttggg
     3541 ggccaacgcg ccgacgatgc cctcggcaac cagttccccc atccggtcga cgggatagac
     3601 cgtgttgatg tcgcgctgga aacctccccg atcatagttg atcgagatat ggctcatcac
     3661 gaggtccgag gcgggcgtac tggccggaag ctttcggaat tcagccgagc cggccgcgaa
     3721 tggcggcgtc gatcgaggat ggagtgctgc gctcgtgacg agggcaatcg tcgagcggaa
     3781 gagcggcttc ggcgcgaccc acgggctcgt ggtcggctcg gcgattgcga gacccgcgac
     3841 ctgatcacgg gtcggttggt agatatcctc gatgcgagcc atacatagaa ccttgtgagc
     3901 tgatgaaggg tggttcgcga tcgtcggcac agcgctgaca ctcggtcctt cgcaattggt
     3961 ccgcggtcgc tgacggtcgc ggcaggatac ttcagtactt gcggcccgtg gtgatgtcgg
     4021 tgccctgcgg gccgacggcc tcagtgtgca tctcgccagc ggcgacctcg aaggtctggc
     4081 cggaggtata gcttcgcggc gtcccgttgc gggagatgat gaactcgccc gctgtcacga
     4141 gccctcgcac ggcgaactcg tgggcgtgct cgccgttctc gggcttcggc tcnnnnnnnn
     4201 nngcgacatg acatcctggt agcctgacga cttcagctcg gtttcgaatt gcgcgggcgt
     4261 catgactggc tcgcttggtt ggcacagagc acgcggctcg gagggctggc tgacgatgcg
     4321 actagagcga agtaccggct gcttgtcgag gccctgttgc tctcacggag caagtggtcg
     4381 tatgcgcggc ctgcatctcg cacgtgcgag catgtcgcag cgagacatgt tgcaatgcaa
     4441 taaatccggg actatatact ggcgagttga tggccggagc ggcagtgcgt gagggtgcga
     4501 tgagcaagaa gatcagcaag acgaagacga tggcggacct cgaggccaac gattgccgct
     4561 ggccgatcgg agatccgcgc gaggccaatt tccatttctg cggcgcccag aaggtgctcg
     4621 gcaggcccta ctgcatggag cattggacgc tgtcgttcga cgcgaccaag acgcggcggc
     4681 cgtcctcggc gccgatgcta cccattcgca gggcggcctg atacgcttcc gccgtagagg
     4741 ctggcgtgtg ccgatggata ttcgcggcgg gtgaggcatg gcctcgcccg ccgtcgtcat
     4801 gtctgcattg ccttcccaga gcgcggccgg cgtgccaact tccaatccgg aaacgcaatc
     4861 gcccgagagg aaggccatgt ccgacaccta cgaggtgctc gccgtcaagt acggcacgca
     4921 cgccaatcgt ccgcggcacc tgaatttcat gaatgccgac gaccacgcga gcctgcagcc
     4981 gatcgactat ttcgtctggg tggtccgcaa tgcgcatcac accgtcgtcg tcgatacggg
     5041 cttcacacat gccgcggnnn nnnnnnnnnn nnacatggag ccgatcgagg ctctgaaggg
     5101 catcggcgtc gacgcgggca aggtgccaca cgtcgtcgtc actcatctcc actacgacca
     5161 cgccggcacg ctcgagcatt ttccaggcgc ccgcttccac cttcaggagg cggagatggc
     5221 gtatgcaacc ggccgctgca tgtgcgacga ggccatgcgt ctgcccttca ctgccgatca
     5281 tgtctgccag atggtgcagc gcgtctactc gggccgcgtt cagttccacg agggcgacgg
     5341 cgaggtcgta ccgggcgtta ccgtgcacaa gtgccccggc cacagccagg gcctccagtc
     5401 ggtccgtgta aagaccgaca acggctatgt cgtcctcgcg tccgatgccg cccatctcta
     5461 tgagaacatc gagaagcgga agcccttctc catcacggtg aacatggaac agacgctgcg
     5521 cacctacgac aagctcgcgg cgcttggcgg cggcctgaag aacgtggtgc ccggacacga
     5581 cccgcaggtc ttgaagatct atccggcctg gaagccggag acggccggcc gggtggtccg
     5641 cctcgacaag gggcggatcg cctgagcgag gaatcggttt cgacatgggt gcggcgcgcg
     5701 tcgtacccgt cgagccgatt tagcaacttg ggagtttggt gcgcccgagg tggcgagaca
     5761 gagccggcac tgtcgcgagg ctggatcggc cggcctctgc atctcggttt tcgtgattcg
     5821 atcgcgaagt tcgctgccgt gtgcggcaca ctcaggcgag cttcgcgatc gacaaaggca
     5881 ctataaatat catgagccta gtgtggcatc ttgttcggag gctcttggcg cgggcatgcc
     5941 ccgcagtgaa cacgatcgtc cgaaccgcga cacgagttca ggaactcatc tgatctgcca
     6001 catggcgctg tgccatcccg gcaggatcgg gccggcttga atggcacccg tttcgacatg
     6061 caagtagtgg tcggccttcc atcgattccg caggcggacg aaatttggga ggccaggaac
     6121 tggctcgata acccacatgg cgctgtgcca gcctggctca atcgggccgg cctgaacggc
     6181 agccttctcg acatggatgt aatggttccc cttccatcga ttgcgcagcc gcacgtggtt
     6241 gggcactccc gggaccggct cgatgaccca catggcactg tgccatcccg cctcgatcgg
     6301 gccggcctcg atgcgaccac gctcgacgtg aacgaaatgg ttgggcttcc agcgattctc
     6361 aacccttaca tacgattcgg ctgccgccgg cgggagtacg aggaacacgc cgagcgcaag
     6421 cgccagcagc ccgccaaaca agcccgtcac gttgttcttt ttcatgcaat tccccatata
     6481 tctcgacagt ccgtgcggtc gtcatcccgc ggttacgtac tcgcgtcggc cgaaaccggt
     6541 gttgcacgat tcaggcgact aacagtacgt ccgcaagaac ggtggcacta gtcacgctcg
     6601 tcgaatatcc caaagttttc gagccgccgg acaatctgcc ggcgacatgt cttcagcgga
     6661 caaggaccaa tcggccgcgg ctacacgccc tggcggcgcg ccgttgcctg gtgactgccc
     6721 cggggactag gctgatctgt cgaactgtcg caaggaatac cgcacaattc cgcagggcta
     6781 tgcagtagtc ctctttcctc ggatagttgc cttgatctgt ctttcgcgcg tcttgccttg
     6841 atgtcggcgc cgcgctttcc tacagtcggt gtggtcgagc cgaggggtgc agtcccgcgg
     6901 cagggggaac cgggagctcg gatgacgatc ctcagatgag cgagcagagc aagaacttcc
     6961 cggagttcta cgtcacggcg ccgcatccgt gcccctacct gcctgggcgc gcggagcgca
     7021 agctcttcac gcacctcacc cacgataagc cggccttcct cgttgacaac ctcctgaagg
     7081 gtggcttccg ccgcagccag aacatcgcct acatgcccta ctgcgagggc tgccaggcct
     7141 gcgtttcggt gcgcgtcatc gtcgacgagt tcgtgccggg ccgcaccttc cggcgcacga
     7201 tgctgggcaa tcgtgatctc gagatctccc gcatcgaacc ggtgccttcg gccgagcaat
     7261 acggcctctt ccgcagctac atcgatgccc gccacggcga aggcggcatg gccgacatga
     7321 cggtgctcga ctacgccatg atggtcgagg acagcatcat cgagaccttc gtcaccgagt
     7381 accggttgag gcccaggagt ccgcactcgt cacggccgtc gacctggccg ctgctcggcg
     7441 tcgccctttg cgatcgtctc tccgacggca tctcgatggt ttacagcttt tacgataccg
     7501 aggctcagga tcgcggtctc ggcacgttca tgatccttga tcatatcgcc tacgcgcgac
     7561 gtctgggcct cccctacctc tatctcggct actggatcga gggatcgcgc aagatggcct
     7621 acaagcgccg gttcatgccg caggagcaat tgacgggtgg cggctggact cgagtcgagg
     7681 cctgaggctt cgacaggcaa ctctggcatg tccgcggctc atgcccgcgg cccatattga
     7741 atgcgaccgc tcgaagcctt atagtgcgcc ccgtgcaaat gcggaccaac accaacgcag
     7801 ccccgagacc ggccaagctg gtccatcgag cggctgcgtt ttgctttgca cggccgaacg
     7861 gccccaagtt caccaccgag aggagtttca ggccacgatg cgcgccactg ccggagcatc
     7921 ccgcaagcga tccgtcccag cacccaagac caagggcagc gatgctctcc tcggtctcgt
     7981 cacgacctgt ctcgacgacg ccaaggccga caacatcgtc accatcgatc tagccggcaa
     8041 atcatcgctc ggcgactaca tggtgatcgc ctcggggaac tccgaccgcc acgtcggtgc
     8101 cattgccgac cagctgcagc gcaagctcaa ggaggccggc cgcgtgcgcg tgcgcgttga
     8161 ggggcaggac cactgcgatt gggtgctggt cgatgccggc gacgtcatcg tccacctgtt
     8221 ccggcccgag gtgcgcgact tctacaacct cgagaagatg tggtccgccg agcgcgccgg
     8281 cgagacgacg cagcactgag ggcgtacgcc gcgtcccatg cgacttctgc tcgtggcgat
     8341 cggtcgattg aaggatggcc cggagcgcgc gctgttcgag cgctattgga cacggctcgc
     8401 cgccatgtct cgacccctcg gcatcaccgc gatcgggttc aaggagctcg ccgagagccc
     8461 ggcgcgggac gcaacgaccc ggaaggcgga cgagggccgg cgcctgatcg agacggccgc
     8521 cgggctcgtc accatcgtgc tcgacgagcg cggcaaggcg atgagcagcg aggccttcgc
     8581 atcgcgactt cagtcgatgg cgggttcagg cgggtcgggc ctcgccatcc tggttggagg
     8641 accggacggt cactcccccg aggttctggc tggcgcacgc ctcaagctct ctttcggcgc
     8701 catgaccttt ccgcatggcc tcgcccgcat cctgatcgcc gagcagctct atcgtgccgc
     8761 gaccatcgcc gcggggcatc cctaccacag gtcctgaccg caaggggcgg ccgcgcatgt
     8821 accgaacgct cgacgccgca aagctcatcg tctcgctcga cacgctcggc cgtcgcatcg
     8881 gcgagcgttt tcccgatgca agcttgaggc aggtcggcac cgatctcgct gacatcgcgc
     8941 gtgagcattc cgagcgcgcg cgccagatca gcggcgcgaa ctggccgctg agagccttcg
     9001 tcgcgattgt tctcgccggc ggcctctggc tgatcgtgcg gatcgtcggc ctcatggacc
     9061 tctccaagac cgcggcagac aacgtctaca cggtgctgca aggcgtcgag gcgacgttga
     9121 acatcgtcgt tctcatgggc ggcgcgctgt tcttcctcat caccgtcgag gaccgtctga
     9181 agcggcgccg cgcgctgatg gcgatccacg agctgcgctc gattgtccac gtgatcgaca
     9241 tgcaccagct gaccaaggac ccttctgggc tcctccagaa tccggggcag ggcgatacac
     9301 cctcctcgcc gaggcgcacc tataccccgt tcgagctcac gcgctatctc gactactgct
     9361 ccgagatgct ctcgctcgcc gccaaggtcg cagcgctcta cgcacagagc tatcccgacc
     9421 cagtcgtgac gagctccgtc aacgaactcg agcagcttac cgccaatctt tcgcagaaga
     9481 tctggcagaa gatctcgatc gtcgagcaga tgatcgcgaa catgccgcgc gcctcagtgc
     9541 cgagcgtgaa accggcggcc acgctgaagc cggatggaac aaaagccgcg cctgtga
//
LOCUS       DAORNH010000170         9572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5272_length_9572_cov_2.571083, whole genome shotgun sequence.
ACCESSION   DAORNH010000170 DAORNH010000000
VERSION     DAORNH010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9572
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5272_length_9572_cov_2.571083"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PK264_16070"
     CDS             <1..300
                     /locus_tag="PK264_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77424.1"
                     /translation="MRVVLSGNRIEIKYVQPRSGMAAEGVGSGEVLFHGTISDGTISG
                     TAYRFSRQCGKRGYSVRGTVSNGTHIVLKGTATRIDRNTCRAVETFADTLEFSAN"
     gene            complement(287..880)
                     /locus_tag="PK264_16075"
     CDS             complement(287..880)
                     /locus_tag="PK264_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD77425.1"
                     /translation="MRTADVDILIIPGWTNSGPDHWQSRWQRNLKTARRVEQADWDTP
                     KVGDWVERIAAAVANASRPAVLVAHSCGVTAVAHAASALPREKIAGAFLVAPTDPEAA
                     PDIWPHASGGFSPLPLARLPLPSRVIASSNDPYCSVERAQVIAAAWGADISIIANAGH
                     INTDSGHGPWPEGLLSFGLFLKSLGESPAKAPTVSSH"
     gene            complement(918..>1194)
                     /locus_tag="PK264_16080"
     CDS             complement(918..>1194)
                     /locus_tag="PK264_16080"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRD77426.1"
                     /translation="MSSGTTFRPDRPAGRLVTDGPYRFRRHPIYIADALILLGLAELS
                     KNIWLVILTPVFAVLVTWLAIRPEERHLEARFGDEWRAYRDRTRLWL"
     assembly_gap    1195..1244
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1605..1614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1615..2250
                     /locus_tag="PK264_16085"
     CDS             <1615..2250
                     /locus_tag="PK264_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD77427.1"
                     /translation="DDCSDDATGDEVKALIATGRFPGLRYLRHATRSGQSTAMRTGIL
                     AAGAPIIATLDGDGQNDPADIPRLLAKLATPGTKGPALVGGVRVERKAEGSKRFASKA
                     ANWIRDRVLRDDCPDTGCGIKVYWRDAFLRLPFFTSIHRYLPALFITYGYEVAYLPVN
                     DRPRQAGVSKYNNIGRALIGLYDLVGVSWIRRRTKAPAIAEEAGKAADPPV"
     gene            2403..2708
                     /locus_tag="PK264_16090"
     CDS             2403..2708
                     /locus_tag="PK264_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HRD77428.1"
                     /translation="MAQWWAATPTIEKTWLAIGFIAQLMFSMRFILQWIASEKARRSI
                     VPETFWYFSVAGGLLLLAYAIYRVDPVFILGQASGLAIYSRNIYFIWKGKSGEAPPA"
     gene            2724..3419
                     /locus_tag="PK264_16095"
     CDS             2724..3419
                     /locus_tag="PK264_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77429.1"
                     /translation="MKHVSVIQHTQSEWLGHIEDHLEGRGIRFGYFRPFTAGGSLPEL
                     ATLGDGLILVGGGPWGTAGPDCLLPTLEREVRLVRACLMTEKPVLAFGLGSQILSLAA
                     DGGVVARPLRLEAGYANRVKGDALGGRLPERFPNVVYMRDAPAPPDYAVALARDAGGN
                     PAIFQIGAKAFGFTGHLGIRRAMIEDLVMEFEEAPDDVAAGLEPLGGLATAIEDALVP
                     IMAGLIEATGWMS"
     gene            complement(3435..4100)
                     /gene="metW"
                     /locus_tag="PK264_16100"
     CDS             complement(3435..4100)
                     /gene="metW"
                     /locus_tag="PK264_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HRD77430.1"
                     /translation="MANAASLAHHAGDISRTAASSRVDHLLITEMVRPGARVLDVGCG
                     DGALLQLLADRKAVDGRGIEITREKVNACVARGLSVIQGNADHDLVDYPDNAFDYAIL
                     SLTIQATLHPKLVLEQLLRIGQRAIVSFPNFGHWQVRMKLLFTGRMPVTENLPESWYA
                     TPNAHLCTIKDFADLCAAVGARVEQAAAFNSSGQQLGTWVRVPLTVHNFLGEKAVFLL
                     SRR"
     gene            complement(4131..4613)
                     /locus_tag="PK264_16105"
     CDS             complement(4131..4613)
                     /locus_tag="PK264_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRD77431.1"
                     /translation="MIPLSAIADRIGARLTERKETIAVVESSAGGLISAALLARPGAS
                     AYFLGGAVVYTRQARTALLAITDAEMKGMRSSSEPYAALIARVMRHRHGATWGLAETG
                     ASGPTGNRYGDAAGHTCIALSGPVELALTLETGHGKRDENMLAFAERALKELERAIGA
                     "
     gene            complement(4610..>4761)
                     /locus_tag="PK264_16110"
     CDS             complement(4610..>4761)
                     /locus_tag="PK264_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD77432.1"
                     /translation="LICRAVAGDTIAVCPPLIIDGAGCNQMFDALGKALDRGLAWAKK
                     EGLLA"
     assembly_gap    4762..4771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4772..>5759)
                     /locus_tag="PK264_16115"
     CDS             complement(<4772..>5759)
                     /locus_tag="PK264_16115"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRD77433.1"
                     /translation="RSHEPAIQLAEKIKEISPAPTSKVFFTSSGSEANDTQIKLAWYY
                     NNARGLMKKKKIISRMRAYHGVTIAAASLTGLPVFHADFDLPMARVLHADTPHHWRGA
                     EAGESEEDFATRLAQRIEELIEREGPETVAAFIAEPVMGAGGVIVPPRTYFEKVQGVL
                     RKHDVALIADEVICGFGRTGRMFGSQTFNIEPDTITMAKAITSAYMPLGAVTVSEPVY
                     QALVDESKKIGVFAHGFTYSGHPVATAVAVKTLEIYERMNIVAHVQETAPVFQLRLKA
                     LASHPLVGEARGVGLIGGLELVKDKTTKQSFEPKQGVGARVVSVPTDAQGMIP"
     assembly_gap    5760..5769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5770..6028)
                     /locus_tag="PK264_16120"
     CDS             complement(<5770..6028)
                     /locus_tag="PK264_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD77434.1"
                     /translation="MSTLTNFAARDVEALLHPTTNLAAHRANGPLIIERAEGIHVYDS
                     RGKRYIEGLAGLWCTGLGYGNKELVEAAREQMSKLSFTHLFG"
     gene            6526..6951
                     /locus_tag="PK264_16125"
                     /pseudo
     CDS             6526..6951
                     /locus_tag="PK264_16125"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
     gene            complement(7022..7930)
                     /locus_tag="PK264_16130"
     CDS             complement(7022..7930)
                     /locus_tag="PK264_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77435.1"
                     /translation="MADDVPFKKTMEFAYGEPREMAPGIVRLVANNPGPFTFKGTNTY
                     LVGTGHSVMLIDPGPEDDAHFNAIMAALGPRKLSHIVVTHTHRDHIDGLPRLAKATGA
                     PVAAYGRTATATPGAKSPSGGEAFNGSFTPDIVLRDGDLVSGDGSALKCIFTPGHAPD
                     HLCFELVGTKLVFSGDHVMSWNTSVVAPPEGHMGDYMRSLFRLLERQDEMFLPGHGGR
                     FEQPQRLVKAFIIHRQMREQAILDCIGNGHDTIDAIVPIVYRGLEPRLVNAASLSVEA
                     HVRHLQEQGRLQPLQHLERSTRLTRP"
     gene            complement(8027..8392)
                     /locus_tag="PK264_16135"
     CDS             complement(8027..8392)
                     /locus_tag="PK264_16135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRD77436.1"
                     /translation="MNALLMQYLPIAVFLGVAIFIGLALMIAPFLIAVRNPDSEKVSA
                     YECGFNAFDDARMKFDVRFYLVSILFIIFDLEVAFLFPWAAAFKDVGVYGFWSMMVFL
                     AVLTIGFIYEWRKGALDWD"
     gene            8570..>8771
                     /locus_tag="PK264_16140"
     CDS             8570..>8771
                     /locus_tag="PK264_16140"
                     /inference="COORDINATES: protein motif:HMM:NF016210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HRD77437.1"
                     /translation="MSQKRIPAVFMRGGTSKAVMFHVRDLPADREAWAPVFLEAIGAP
                     DPNGRQLDGMGGGVSSLSKACII"
     assembly_gap    8772..8846
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8860..>9178
                     /locus_tag="PK264_16145"
     CDS             8860..>9178
                     /locus_tag="PK264_16145"
                     /inference="COORDINATES: protein motif:HMM:NF016210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HRD77438.1"
                     /translation="MSSAIGPFAVDEGLVPAPAGDEATVRIHNTNTAKIIKSHFPVVG
                     GKAAVRGDLAIDGVSGTGAPIRLEFMAPGGSKTGELLPTGQPVDRLPLASGRYVDVSL
                     VDAA"
     assembly_gap    9179..9242
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9243..>9572
                     /locus_tag="PK264_16150"
     CDS             <9243..>9572
                     /locus_tag="PK264_16150"
                     /inference="COORDINATES: protein motif:HMM:NF016210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HRD77439.1"
                     /translation="ALEAMPEALATLEEIRARASVAMGLAPNLSNAARLKSIPKIAVV
                     TSPRAATTLSGRELGAADMEIAARMISVGQPHRAIPLTGALCLAVATRTPGTIPNRLA
                     RGLPPGA"
BASE COUNT         1579 a         3042 c         3176 g         1556 t
ORIGIN      
        1 atgcgcgtcg tcctcagcgg caaccgcatc gaaatcaagt acgtgcagcc gcgctccggc
       61 atggcggctg aaggcgtcgg ctcgggcgag gtgctgttcc acggcacgat ctcggacggc
      121 accatctcgg gcaccgccta ccgcttctcg cgccagtgcg gcaagcgcgg ttactccgtg
      181 cgcggcaccg tcagcaacgg aactcacatc gtgctcaagg gtaccgctac gcgcatcgac
      241 cgcaacacct gccgcgccgt ggaaacgttc gccgacacgc tcgagttcag tgcgaactga
      301 cggtcggggc cttcgccggg ctctcgccga gcgacttgag gaagaggccg aacgagagga
      361 gcccctcggg ccaggggccg tggccagagt ctgtgttgat gtggcccgcg ttggcgatga
      421 tcgagatgtc cgcaccccag gccgcggcga tcacctgcgc gcgctcgacc gagcagtatg
      481 gatcgttcga gctcgcgatg acgcgtgagg gcaatggcag ccgcgcgagc ggcagcggtg
      541 aaaagccacc ggacgcgtgc ggccagatgt caggcgcggc ctccggatca gtcggagcga
      601 cgaggaacgc gccggcgatc ttctcgcgtg gcagtgcgga tgctgcatgc gccacggccg
      661 tgacgccgca gctgtgtgcg acgagtaccg cggggcgcga ggcgttcgcc accgcggcag
      721 cgattcgctc gacccaatcg ccaaccttcg gcgtatccca gtccgcctgc tcgacacgcc
      781 gcgcggtctt gaggttgcgc tgccagcggc tctgccaatg atccggcccg gagttggtcc
      841 agcccgggat gatcaggatg tcgacgtcgg cggttcgcat ctctcaatcc ttaccctctc
      901 gaatgccaat ccaccggcta cagccagagg cgtgtccggt cacggtacgc gcgccattca
      961 tcgccgaacc gcgcttcgag atggcgttcc tcgggccgga tggcaagcca cgtgacgaga
     1021 accgcgaaca ccggcgtcag gatgaccagc cagatgttct tgctgagctc ggcgaggccc
     1081 agcaggataa gcgcatcggc gatgtagatc ggatgacgtc gaaagcggta ggggccgtcg
     1141 gtgacgaggc gcccggccgg ccgatcgggc ctgaacgtcg ttcccgaact catgnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnataccg acgattctgg
     1261 caccgagatc gtcgaggccg ggccatccga gcggtcggag ccgagcgagc ccgtaagccg
     1321 ccgcaatggc ggcggcgagc aggagcggag gccatggcac gcgcgatggc ggggcatgcg
     1381 cactcgtctc gtctgtcgcg tctcgcatca cgaggcccgt tgtcggtgtg aagtgttcga
     1441 ggtttccgtt ttacaagctc gggagcccat gacaaaccgg ctccgaacca gcagcacggg
     1501 cctcgatgca aatcgccatc gtcatccccg ccttcaacga ggccggcaac atcggccggc
     1561 tcgtggagga ggcatttcat agtgttccgc gcgagcggct cgccnnnnnn nnnngacgac
     1621 tgcagcgacg atgcgacagg cgacgaggtg aaggcgctga tcgcgacggg ccgcttccct
     1681 gggctgaggt acctccgcca cgcgacgcgc agcggccaga gcacggcgat gcgaaccggc
     1741 atcctggccg caggcgcgcc catcatcgcg acactcgacg gcgacgggca gaacgatccc
     1801 gccgacatcc cgcggctcct cgccaagctt gccacacccg gcacgaaagg tccggcgctg
     1861 gtcggcggcg tcagggtgga gcgcaaggcc gaaggctcga agcgcttcgc ctccaaggcc
     1921 gcgaactgga tccgcgatcg cgtgctgcgc gacgactgcc cagatacagg ctgcggcatc
     1981 aaggtctact ggcgcgacgc gttcctgcgt ctccccttct tcacgagcat ccaccgctac
     2041 ctgccggccc tcttcatcac gtacggctac gaggtggcct atctgccggt gaacgacagg
     2101 ccgcggcagg cgggcgtctc caagtacaac aacatcggcc gcgcgctgat cgggctctac
     2161 gaccttgtcg gcgtctcgtg gatcaggcgc cgcaccaagg cgcctgccat cgccgaggaa
     2221 gccgggaagg ccgctgaccc tccggtctga gcaaaagtca cgagagacca gctgtcgcgg
     2281 gagaaaatcg cgtctgcacc tcgacagaca gggcggggcg gggcacgaag cgctgtaaca
     2341 gcaagccgaa acgtggctgc agagggctcc gcatggattt cttcacgacg cttgccgcca
     2401 agatggctca gtggtgggcc gcgacgccca cgatcgagaa gacctggctc gccatcggct
     2461 tcatcgccca gttgatgttc tcgatgcgct tcatcctcca gtggatcgcg agcgagaagg
     2521 cgcgccgttc gatcgtgccg gagacattct ggtacttctc ggtggcgggc ggcctcctcc
     2581 tgctcgccta cgcgatctac cgggtcgatc ctgttttcat cctcgggcag gcgtcagggc
     2641 tcgccatcta ctctcgcaac atctatttca tctggaaggg caagtccggc gaggcgccgc
     2701 cggcctgagg cgacagcgaa ctcatgaagc acgtctccgt catccagcat acccaatcag
     2761 agtggctcgg tcacatcgag gaccatctcg agggccgcgg catccggttc ggctacttcc
     2821 ggccgttcac ggcgggcggc agcctgcccg agcttgcgac cctcggggac gggctcatcc
     2881 tggtcggcgg cggaccatgg ggcacggcgg ggccagattg cctcctcccg acgctcgagc
     2941 gcgaggtgcg gctcgtgcgc gcctgcctca tgacggagaa gccggtgctg gccttcggcc
     3001 tcggcagtca gattctctcg ctcgccgctg acggcggcgt cgtggcacgc ccgctgcggc
     3061 tggaggccgg atacgcgaac cgcgtcaagg gagacgcgct cggtggccgg ctgcccgagc
     3121 gctttcccaa tgtcgtctac atgcgcgatg cgccggcgcc gcccgactat gccgtagcgc
     3181 ttgcccgcga cgcaggtggt aacccggcga tcttccagat cggtgccaag gcattcggct
     3241 ttaccggaca tctcggcatc cggcgcgcca tgatcgagga tctcgtcatg gaattcgagg
     3301 aggcgcccga cgatgtggca gctgggctcg agccgcttgg cgggctcgcg acggcgatcg
     3361 aggacgcgct cgtccctatc atggcaggcc tcatagaggc gacgggctgg atgagctgag
     3421 gcgacgtgac gcgctcagcg ccggctcagc agaaagaccg ccttctcgcc gaggaaattg
     3481 tgcaccgtga gcggcacgcg cacccaggtg ccgagctgct ggccggacga gttgaaggcg
     3541 gcggcctgct cgacgcgggc gccgacggct gcgcatagat ccgcgaaatc cttgatcgtg
     3601 cagaggtgcg cgttcggcgt cgcataccag gactcgggca ggttttccgt caccggcatg
     3661 cgcccggtga acagcagctt catgcgcacc tgccagtggc cgaaattcgg gaacgagacg
     3721 atcgcgcgct ggccgatgcg caggagctgc tcgagcacga gctttggatg cagcgtcgcc
     3781 tggatggtga gcgagaggat cgcatagtcg aaggcgttgt cgggatagtc gacgaggtcg
     3841 tgatcggcat tgccctggat gacggagagg ccccgcgcca cgcaggcgtt gaccttctcg
     3901 cgcgtgatct ctatgccgcg gccgtcgacg gccttgcgat cggcgagcag ctgcaggagc
     3961 gcgccgtcgc cgcagccgac atcgaggacg cgcgcccccg gcctcaccat ttcagtgatc
     4021 agcaggtggt cgacgcggct cgacgcagcc gtgcgggaga tgtcgccggc atgatgtgcg
     4081 agggaagccg cgttggccat gtcagaagca ccttccatat aggctgcccg tcacgcgccg
     4141 atcgcgcgct cgagttcctt aagcgcccgc tcggcgaacg caagcatgtt ctcgtcacgc
     4201 ttgccatgcc ccgtctccag ggtgagcgcg agttcgacgg gacccgacaa tgcgatgcag
     4261 gtgtggccgg cggcgtcccc gtagcggttg ccggtcggcc ccgaggcgcc ggtctcggcg
     4321 aggccccagg tggcaccatg gcgatggcgc atgacgcggg cgatgagcgc cgcatagggc
     4381 tcgctcgagg agcgcatgcc tttcatctcg gcgtccgtga tggcgagcag cgctgtgcgg
     4441 gcctgtcgcg tataaaccac ggcgccgccg aggaagtagg cagacgcgcc gggtcgggcg
     4501 aggagggccg ccgagatgag gccaccggcg gacgattcga cgacggcgat ggtttccttg
     4561 cgctccgtga ggcgagctcc gatccggtcg gcgatggcgc tgagcgggat catgcgagca
     4621 gcccttcctt cttcgcccag gcaaggcctc ggtcgagtgc cttgccgagc gcatcgaaca
     4681 tctggttgca gcccgcgccg tcgatgatga gcggcggaca gaccgcgatc gtgtctccgg
     4741 cgactgcgcg gcaaatgagc cnnnnnnnnn nggggatcat gccctgggca tcggtcggga
     4801 cggagacgac gcgggcaccg acgccctgct tcggctcgaa cgactgcttc gtcgttttgt
     4861 ccttcacgag ctctagcccg ccgatgaggc cgacaccgcg cgcctcccct accaacgggt
     4921 ggcttgcgag cgctttgagt cgcagctgga acaccggcgc cgtctcctgc acgtgggcga
     4981 cgatgttcat acgctcgtag atctcgagcg tcttcacggc gacggcggtc gcaaccggat
     5041 gacccgagta cgtgaagcca tgtgcgaaga cgccgatctt cttgctctca tcgacgagcg
     5101 cctgatagac gggctccgag acggtcaccg cgccgagagg catgtaggcg gaggtgatgg
     5161 ccttcgccat cgtgatcgta tccggctcga tgttgaacgt ttgcgatccg aacatgcggc
     5221 ccgtgcgccc gaagccgcag atcacctcgt cggcgatgag cgcgacgtcg tgcttcctga
     5281 gcaccccctg caccttctcg aaataggtgc gtggcggcac gatgacgccg cccgcgccca
     5341 tcaccggttc ggcaatgaag gccgcgaccg tctccggccc ctcgcgctcg atcaactcct
     5401 cgatgcgctg ggcgaggcga gtcgcgaagt cctcctcgct ttcgccggct tcggcgcccc
     5461 gccagtggtg cggcgtatcg gcatgcagca cgcgcgccat cggcaggtcg aaatcggcgt
     5521 ggaatacggg gaggccggtg aggctcgccg ctgcgatcgt cacgccgtga taggcgcgca
     5581 tgcgcgatat gatcttcttc tttttcatca ggccgcgggc attgttgtag taccaggcga
     5641 gcttgatctg cgtatcgttg gcctcgctcc ccgagctcgt gaagaaaacc ttggaggtcg
     5701 gcgcgggaga gatctccttg atcttttcgg cgagctggat cgcgggctcg tggctgcggn
     5761 nnnnnnnnnc gccgaagaga tgcgtgaagg agagcttcga catctgctcg cgtgcggctt
     5821 cgacgagctc cttgttgccg tagccgaggc ccgtgcacca caagccagcc aagccctcga
     5881 tgtagcgctt gccgcgcgaa tcgtagacgt gaatgccttc ggcccgctcg atgatcagcg
     5941 ggccgttcgc ccggtgcgcg gcgagattgg tcgtcgggtg aagcagcgcc tcgacgtcgc
     6001 gcgccgcgaa attggtgagt gtcgacatct ctgctcgtct ccggtctggc cgaggcctca
     6061 atcgccaact atggtaccgg cggtccgtga cgaagacaaa agattcgcaa ccggggtggt
     6121 aagtgccgag agcgtcatcg ggaatgaact tggtccgtcg cgggcgagac accaagccaa
     6181 ggaattgaga tgatcagaag cggcccgatc accactgcgc gcgcgctggc actgtcgact
     6241 cccttgcccc tgccgattgc gtcctggtcg cgcacgcttg cggtcttcgc cgtgcaactc
     6301 gtgctgctga cgatcctgct ccaccgcttc gcctcgctgc cgacgcccgt tgcactgaat
     6361 gttttcatcg ttgcgctggc aatggccgcg attgccttac tgatcggctt caccgcggcc
     6421 gcattcatct gggtgcaggg tcgcgacggc gccttcaatg cgaccgtcgg cgtcggcttg
     6481 agccttgccc tgatgatgtg gccggctgcg gctgcgccct tctacatgcg cctgccgcgc
     6541 atcaacgaca tcaccaccga taccgtgaac ccgcccgtgt tccaggcgct cgccaaggcg
     6601 cgacccaagg gcgccaacat cgtcgcctac ggcggcaata cgatcgccgc acgccagatc
     6661 gccgcctacc cggatgtgcg ccctgtcatc gttccgcggg gagccgagga gacccacgag
     6721 atcgtgctcg acgtgatgcg gcgtctcaag tggcgggtgg tagcagagga ggcgccgagc
     6781 cgtggcaagc cgggtctgat cgaggccgtc gaccggactc tgatcatcgg cttcaatgac
     6841 gatatcgtcg tcaggatcac gggcgatgcc acacgcaccc ggatcgacgc gcgctcgcag
     6901 tcgcgctacg gcggacacga tctcggccgc aatgcgcgcc gcgtgacgag cttcttcaag
     6961 gaagtgatcg cccgcttcga cgggaccggc ggcgagcgcg tcaggcgccg gcgcggctcc
     7021 actaaggccg tgtcagacgc gtcgaccgct ccaagtgctg caaaggctgc agccgcccct
     7081 gctcctggag gtgacgcacg tgagcctcga cggaaagtga agctgcgttc acgaggcgag
     7141 gctcgaggcc gcgataaaca ataggcacga ttgcgtcgat ggtgtcgtgg ccgttgccga
     7201 tgcagtcgag gatcgcctgc tcgcgcatct ggcggtggat gatgaacgcc ttgacgaggc
     7261 gctgcggctg ctcgaagcgc ccgccgtggc cgggcaggaa catctcgtcc tgccgctcca
     7321 ggaggcggaa gagcgagcgc atgtagtcgc ccatgtggcc ttcgggcggt gcgacgaccg
     7381 aggtgttcca cgacatcacg tgatcgccgg agaagacgag cttggtgccg acgagctcga
     7441 agcagaggtg atcgggcgcg tggcccggcg tgaatatgca cttgagggcg ctgccgtcgc
     7501 cgctgacaag atcgccgtca cgaagcacga tatcgggcgt gaacgagcca ttgaaggcct
     7561 cgccgccgga tgggctcttc gcgccaggcg tggcggtggc cgtgcggcca taggcggcaa
     7621 cgggtgcgcc ggtcgccttg gcgagccgcg gcaggccgtc gatgtgatcg cgatgcgtgt
     7681 gcgtgacgac gatatggctc agtttgcgag gtccgagcgc cgccatgatg gcgttgaagt
     7741 gggcgtcgtc ctcggggccg ggatcgatca gcatgaccga gtggccggtg ccgacgaggt
     7801 aggtgttggt gcccttgaac gtgaacgggc ccgggttgtt ggcgacgagc cgcacgatcc
     7861 ccggcgccat ctcgcggggc tcgccgtagg cgaactccat ggtcttcttg aaggggacgt
     7921 cgtcggccat gctgggctcc gctgctcgca gtcgtgcgcc gcaccatagt cgatgcgcgg
     7981 gcggaccgca gctgacttcg ccgcacgccg tcaggagcaa gcgggatcaa tcccagtcga
     8041 gggcaccctt ccgccactcg tagatgaagc cgatggtcag cacggcgagg aacaccatca
     8101 tggaccagaa gccgtagacg ccgacatcct tgaaggcggc ggcccagggg aagaggaagg
     8161 cgacctcgag atcgaaaatg atgaacagga tcgagacgag gtagaagcgc acgtcgaact
     8221 tcatgcgcgc gtcgtcgaac gcgttgaagc cgcactcgta ggcggagacc ttctcgctgt
     8281 ccgggttgcg caccgcgatc aggaacggcg cgatcatcag cgcgagaccg atgaagattg
     8341 cgacgccgag gaagacggcg atcggcagat attgcatcag aagcgcgttc atgtggggtc
     8401 cccgagccgc agcgggctga gcatcatccc cgtcgtctag ccgagcttcg cccgccgttc
     8461 aatgccggaa ctcgtcgcat aggcgccaga cctgtgcgcc atgacttgag gcggcgatgc
     8521 gaatgcccct atcctgcgga ggcgcagccg aaagcgatag gagtgacgca tgtcgcagaa
     8581 gcggatccct gcagtcttca tgcgcggcgg cacctcgaag gccgtaatgt tccacgttcg
     8641 cgacctgccg gccgaccggg aggcctgggc gccagtcttc ctcgaggcga tcggcgcacc
     8701 ggaccccaac ggccgccagc tcgacggcat gggcggcggt gtctcctcgc tctcgaaggc
     8761 gtgcatcatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnncaac tgcgggaata tgtcgtcggc gatcggaccc
     8881 tttgccgtcg acgaagggct cgtgcctgca ccggcaggcg acgaggccac cgtgcgcatt
     8941 cacaacacca acacggcgaa gatcatcaag tctcactttc cagtcgtcgg tggcaaggcc
     9001 gccgttagag gtgatctcgc gatcgacggc gtgtcgggta ccggcgcgcc catccggctc
     9061 gagttcatgg cgccgggcgg ctctaagacc ggtgaactct tgccgacggg ccagccggtg
     9121 gatcgcctgc cactcgcctc tggtcgatac gtggatgtga gcctcgtcga tgcggcaann
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnagccctcg aggcgatgcc ggaagcgctc gcgacgctcg aggagattcg cgcccgagcg
     9301 tccgtcgcca tggggcttgc gccgaatttg agcaatgccg cccgactgaa gagcattcca
     9361 aagatcgccg tcgtgacgag cccacgggcc gccaccacgc tctcggggcg ggagctcggc
     9421 gcggccgaca tggagatcgc cgcgcgtatg atctcggtcg gccagccgca tcgcgcgatc
     9481 ccgctcaccg gcgcgctctg ccttgcggtt gcgacccgga cgcccggtac catcccgaac
     9541 cggctcgcgc gtggtctgcc gccgggggca ag
//
LOCUS       DAORNH010000171         9567 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5275_length_9567_cov_2.682191, whole genome shotgun sequence.
ACCESSION   DAORNH010000171 DAORNH010000000
VERSION     DAORNH010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5275_length_9567_cov_2.682191"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PK264_16155"
     CDS             <1..218
                     /locus_tag="PK264_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77440.1"
                     /translation="EHNLEVIKTADWIIDLGPEGGDGGGRIVAAGTPEDVAKVEASHT
                     GKFLRELLSRRAKSRASRNGGKREAAE"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..985)
                     /locus_tag="PK264_16160"
     CDS             complement(<311..985)
                     /locus_tag="PK264_16160"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRD77441.1"
                     /translation="MDIKIMWVFAFGVGVVLVAIMSLERRLASSGPPSAARGARPFLS
                     RMDYFRSDHTAPLKLFNQLLRRDDWLVIDCETTGFDKKSEILEIAVVDANGTTLFERR
                     VMPLGRIPSAASRVHGIYRKDLKGCPTWPEISEELRRVIGDRPVVSYNGEFEERLLQQ
                     TAEKWGVEPIRNRVGCVMKAYAAHRGIPHPWRRNDFKWHKLAAACRHEGIKVGTHHRA
                     GADAELC"
     gene            1233..1451
                     /locus_tag="PK264_16165"
     CDS             1233..1451
                     /locus_tag="PK264_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77442.1"
                     /translation="MTPETTIKLMREGKYVAEISVTLIDDDNEWSPRLSAGDVQMLDA
                     VRLALRRGDLRAASPLATVYAHAPVAAE"
     gene            1732..2145
                     /locus_tag="PK264_16170"
     CDS             1732..2145
                     /locus_tag="PK264_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77443.1"
                     /translation="MRLRKFLAIAGAAIGLSLAAAAPASAFGWWPHSHVHAPAGWGEV
                     RPVRHWVYYPRYQHIYHVDPYAYRYSPRGYYPWYGSHYWTSAATIRKRNHLKYHHWNA
                     RPPKYRYYPAWSYPKHWHHQDWHARNHGFHHRWHW"
     gene            2289..2597
                     /locus_tag="PK264_16175"
     CDS             2289..2597
                     /locus_tag="PK264_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77444.1"
                     /translation="MMKKIATGAIALTMAGALVVASATEADARRGRRVAGILLGTAIG
                     LGIAGAYAYGPRHHYRGGHCYLGEQRCRVVGQKCWYDRYRGHEVCKDDVRCWRPQICD
                     "
     gene            complement(2669..3838)
                     /locus_tag="PK264_16180"
     CDS             complement(2669..3838)
                     /locus_tag="PK264_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD77445.1"
                     /translation="MSTAPQFNIEPASFALDPYPTLARMRAEAPIAFVPELGSTLLTR
                     RDDIFVCEKNIEVFSSHHPQGLMTRLMGHNMMRKDGEAHMAERRAIFPTVSPKTVAKH
                     WRASFAAHAARILDELRPLARANLTKAFAIRYSAECLKDITGLASMRFEDMDDWSQGM
                     IDGIANYAGDPAIEARCHKATAGIDAAIDDMLPVVRRSPNQSLLSVMLSAGLPMESIR
                     ANIKLAISGGQNEPRDAIAGCVWALLTHPDELALVRAGKATYMQVFDEYCRWIAPIGM
                     SPRRIAKAHSYGGVEFAPEDRVFLMFGSANRDEAHFADADRFDIRRDTAKSIAFGAGP
                     HYCAGAWASRAMVSGVALPMLLERLPGLELDPATPVRVAGWAFRAVQNLPVIWRV"
     gene            4070..4813
                     /locus_tag="PK264_16185"
     CDS             4070..4813
                     /locus_tag="PK264_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77446.1"
                     /translation="MKDFLESSAAILRQYLGFDIPAQHLSIAVGAVSIFSLLIYLLRQ
                     LRLKLGDLSALPSEMLATAEIVKNDIQTNVDALLIKFTALHDEMRLSLHSRFDAIHKL
                     LQATHEVSNQVADSEIAEDEASQRPPRMTRVQYARAIATKVTEQWLSGRTLRSLADSP
                     HVFEFEGALANGQHFKLRFGTPYRQGIGVDGRLPFTLDVWVDGYKHLNYEWDTDGNYA
                     LRGFKKGSGEWLVELAAWGLKSESVAKAA"
     gene            complement(4876..5448)
                     /locus_tag="PK264_16190"
     CDS             complement(4876..5448)
                     /locus_tag="PK264_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HRD77447.1"
                     /translation="MHYEVDRRRLSLLLGAAIATAIGMPCGTAAAAEPHGGPFELIGT
                     DGQPFRSERLAGRPFAIFFGFTNCPEVCPTTLNDLMGVLERLGPDADRIGFLFVSVDA
                     ENDTPAALKEYLSHFDKRVIGLTGHPAEIAAVASRFGAYYERVPTKHGHTYHHSTSMY
                     LVGGDGRRAGEIAYQEDADSQVRKFKALMK"
     gene            complement(5460..5981)
                     /locus_tag="PK264_16195"
     CDS             complement(5460..5981)
                     /locus_tag="PK264_16195"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRD77448.1"
                     /translation="MKPARTLPSTAMALAVLALASVAALAQQTYTIGPIRIDHPWSRV
                     APEGFKVAGGFMRITNTGKEADRLIGGSFIHAARLEVHEMAMKDGRMVMNELAGGLEI
                     PPGGSVLLRPGSFHVMFMELKQPLKSGERIKGTLVFAKAGAIEVEYLIEPLGTQTSGP
                     HAAPKGSGPMDKK"
     gene            complement(6013..6483)
                     /locus_tag="PK264_16200"
     CDS             complement(6013..6483)
                     /locus_tag="PK264_16200"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HRD77449.1"
                     /translation="MIDSRRLLAIGLTTALLLGPVQAHGVKRGAVEIVHPWTHAGANV
                     GDDAAVYMVVKVAAPKGDRLKGASSPIAGSVELHVLDNVAGTHRTKRVSAFEIGARGR
                     LEMTTTGPRLILKQIKKPLSAFDTFAMTLEFERAGRIEVEVLVEDATMVSPPKH"
     gene            7197..8246
                     /locus_tag="PK264_16205"
     CDS             7197..8246
                     /locus_tag="PK264_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD77450.1"
                     /translation="MTLKFHFFTRDTMPDAAVLRSAAHANRMLAEQLPDDPPYDAADE
                     LRRWHAMPSQARSQVWIAEANGNVVAEANLNWEELESNRALAHVYVSVERDWRGQGLG
                     ATLLSLAVERAIEAGRPKLMSMSTSRVPAGGRFLDRVGFTRRLEMHTNQLKVADLDRA
                     LVDAWSRPDPARTGDYAIEVFDGPVPDHRLEAYAELVGVMNTAPRGTLEVEDTKITAS
                     MVREWEASLVASGWRRLLAVTRHVPSGVLAGFTELRWTPTRASLVWQGGTGVVPSHRN
                     RGLGRWLKAQNLAALLSANDKVRFVRTGNADSNAPMLAINHQLGFRPFTARALWQGNA
                     AEVSGQLARQRRQAA"
     gene            complement(8346..9320)
                     /locus_tag="PK264_16210"
     CDS             complement(8346..9320)
                     /locus_tag="PK264_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77451.1"
                     /translation="MRLRPLFLAATVTALSGCAIHPIPDDVSQYTTEDIVRNVRCEAR
                     DSVRTRIKMALDRNPAFAGIEPELILERSDYFARIKKADPLLAEKFLNYADSSIAYDF
                     SFKITEKSDQSGSLSFQLPYPKRTFTLGADGGLGRERSGHRKFKTVETFRELVRLDCR
                     SYVQPDANGLYPLTGSIGMTRVMNTFIELSELGGGKGDFSDVINFTTRIHAKADGKWV
                     LSPLPKTFKLINATALISGERQDIHSLTIGIAFPLKDLRVAAVGPRPVSAFSARASPR
                     IIDTTTRALEQLCIARAEEREDRLGKLRNIPPEIFCRREALAIVGSPM"
     gene            complement(9331..>9567)
                     /locus_tag="PK264_16215"
     CDS             complement(9331..>9567)
                     /locus_tag="PK264_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77452.1"
                     /translation="MQADLGDERRRHEARVVGDEACVLHAGRLCGQRGLSIEKTAQVT
                     RSMKAMRSHLGHRRGAPESVKALGCSGKFRRAHI"
BASE COUNT         1786 a         3084 c         2916 g         1771 t
ORIGIN      
        1 tcgagcacaa tctggaggtc atcaagaccg ccgactggat catcgatctc ggcccggaag
       61 gtggcgacgg cggcggacgc atcgtcgccg ccggcacgcc cgaggacgtc gccaaggtcg
      121 aggcgagcca cacgggcaag ttcctgcgcg agctgctctc tcgccgcgcc aagagccgcg
      181 cctccaggaa cggcggcaaa cgcgaggcgg cggagtaggg aatcctggac gtcattccgg
      241 cgcccgcgcg ccaagcatcg gcagttctaa tgcgatcgat ttctggcaac cgtaagtgcg
      301 nnnnnnnnnn acagagttca gcatccgcac ctgctctatg atgagtgcca accttgatgc
      361 cctcgtgccg gcatgccgcc gcgagcttgt gccatttgaa atcgtttctc cgccaggggt
      421 gtggaattcc acgatgcgct gcgtacgcct tcatgacaca gccgacacga ttcctgatcg
      481 gctcgacacc ccacttttca gcagtttgct gaagcagcct ctcctcgaat tctccattgt
      541 acgagactac aggtcgatcg ccgatgactc tcctcaactc ttccgaaatc tccggccagg
      601 tcggacagcc cttcaggtcc tttctgtaga tgccatgaac cctcgacgcc gccgagggta
      661 tacggccaag cggcatcact ctgcgttcaa atagcgtcgt gccatttgcg tccacgacgg
      721 caatctcgag tatttcggat ttcttgtcga accccgtcgt ctcacagtcg atcacgagcc
      781 agtcatcgcg gcgcaacaat tggttgaaca gcttcagcgg cgcagtgtgg tctgatcgaa
      841 agtagtccat acgagacaga aaaggccttg ctccgcgtgc agcagacggt ggaccggagg
      901 aggctagacg cctctcgaga gacatgatcg caacaagaac gacaccgacg ccgaaggcga
      961 atacccacat gatcttgatg tccatttgag caccctcatt ggaaaatccc ccgccaataa
     1021 accggccgat tcccggtaaa caagatgcta atttttcaac cgccagccta tcacaacgat
     1081 gttcgctgca cgaaacagcg caattctggt tgttctgcag cgatcgggca gcacctgttg
     1141 gttgagttgg atgccaccgg ctaagcgtgc catcgagttc gtgacaatct cttggaatgg
     1201 caattgtaga ccccagactt ccggaggcgg ttatgacccc agagaccacg atcaagctca
     1261 tgcgagaagg caagtacgtc gccgaaatct cggtcacact gatcgacgac gacaacgagt
     1321 ggtcgccccg cctatcggca ggcgatgtgc aaatgctcga tgccgtccgc ttggcattac
     1381 gacgaggcga cttgcgcgcc gcgtcgcccc ttgcaactgt ctatgcacat gcgcctgtgg
     1441 ctgccgagtg aggccacgac ctgcgctggg ggccctgaaa ctcggccaca cgcgccaccc
     1501 gactattcgt acactctaca acagcggcaa tatcctcgag cgccgcgtcg gcctcgtcaa
     1561 gccggctcgc tcaaatcggg cctgagcctc ccgcccgccc ctcatcaccc cgtggccaga
     1621 atgccgcaac ggcccgcgaa catcgagcgc tcgtttactc ttcgttagcc aaaatcatat
     1681 cttctcggag catggccgtc gtcgcggccc ctgataccga ggagtgagtt catgcgtctc
     1741 cgcaagttcc tggcaatcgc cggggcagcg atcgggcttt cgctcgccgc cgccgcacca
     1801 gcgagcgcgt tcggctggtg gccacattcg cacgtgcatg cgccggccgg ctggggcgaa
     1861 gtgcgccccg tgcgccactg ggtctactac ccgcgctacc agcacatcta ccacgtcgat
     1921 ccgtacgcct accgctacag cccgcgcggc tactatccct ggtacggctc ccactactgg
     1981 acatcggccg ccaccatccg caagcgcaat cacctgaagt accaccactg gaacgcccgc
     2041 ccgccgaagt atcgctacta tccggcgtgg agctatccga agcactggca ccaccaggat
     2101 tggcacgccc gcaaccacgg cttccatcac cgctggcact ggtaatttga tcgaaatcac
     2161 ccctcgatta cgttcatgcc acattcaggt gtcatgagcc cttatcggcg gcagttgggg
     2221 acggggtccc cgctgccaac tcgagcaggg tccacaccag cgtggaccgg atacggagga
     2281 cacatcccat gatgaagaaa atcgccaccg gcgccatcgc gctcaccatg gccggcgccc
     2341 tcgtcgtcgc cagcgccacc gaggccgatg cccgccgcgg ccgccgcgtc gccggcatcc
     2401 tgctgggcac tgccatcggc ctcggcattg ctggcgctta cgcctacggc ccgcgtcacc
     2461 actatcgagg cggtcattgc tacctcggcg agcagcgttg ccgcgtcgtc ggccagaaat
     2521 gctggtacga ccgttatcgc ggccacgagg tctgtaagga cgacgtgcgc tgctggcgcc
     2581 cgcagatctg cgattgaacc gcagactgca tgagagtgag cgggcggcgc gcgagcgtcg
     2641 cccgtttcga ttccaggaca cgctcgggtc agacgcgcca gatcaccggc aggttctgca
     2701 ccgccctgaa cgcccagccc gccacgcgta caggcgtcgc cggatcgagt tccaaacccg
     2761 gcaggcgttc gagcagcatc ggcagcgcca cccccgacac catcgcccgt gacgcccatg
     2821 cgcccgcgca gtagtgcggg cccgcaccga acgcgatgct ctttgcggta tcgcgccgga
     2881 tgtcgaagcg atcggcatcg gcgaaatgcg cctcgtcccg gttcgccgat ccgaacatca
     2941 gaaaaacgcg gtcctccggc gcgaactcga cgccgccgta gctgtgcgcc ttggcaatgc
     3001 gtcgcggcga catgccgatc ggcgcaatcc agcggcagta ttcgtcgaac acctgcatgt
     3061 atgtcgcctt gcctgcacgg acgagcgcga gctcgtcggg atgagtgagc agcgcccaga
     3121 cgcatcctgc gatggcatcg cgcggctcgt tctggccgcc gctgatcgcg agcttgatgt
     3181 tcgcgcgtat gctttccatc ggcaggccgg ctgagagcat cacggagagc aggctctggt
     3241 tcggcgatct gcgcaccacc ggtagcatgt cgtcgatcgc cgcatcgatg cccgccgttg
     3301 ccttatgaca gcgcgcctcg attgcgggat cgcctgcata gttcgcgata ccgtcgatca
     3361 tgccttgcga ccagtcatcc atgtcctcga agcgcatgct cgcaaggccg gtgatgtcct
     3421 tcaggcattc ggctgagtag cggatcgcga atgctttcgt gagattggcc cgcgcgagcg
     3481 gccgcaattc gtccaggatg cgcgccgcgt gcgccgcgaa gcttgcgcgc caatgcttgg
     3541 cgaccgtctt cggcgagacc gtcgggaaga tcgcccgccg ctcggccatg tgggcctcgc
     3601 catccttgcg catcatgttg tggcccatga ggcgggtcat taggccctgc gggtgatgcg
     3661 aggaaaacac ctcgatattc ttctcgcaga cgaaaatgtc gtcgcgccgt gtgagcagcg
     3721 tcgaaccgag ctccggcacg aacgcgatcg gggcttctgc acgcatgcgc gcgagcgtcg
     3781 gatagggatc gagggcaaag ctcgcgggtt caatgttgaa ctgaggtgcc gtggacatga
     3841 gggtctcgct ggtctgcgct gtcgcaccgg gccgctgccg gttccagatc agtaccgtca
     3901 gaagctacgg caaaagcctg tgcgatagcc agggggcgca atcgaaccct cgattgtcgc
     3961 cgtcatgtcg tcatgccgca acttgccagc catcgatcaa tgaggttaac tgctggcttc
     4021 cgctcacaga cgatcagaaa gtgcgatcgc gagtggcgta gggggcagca tgaaggattt
     4081 cctggagtcg tcggccgcaa tcttgcgcca atatttgggt ttcgacattc ctgcgcagca
     4141 cctctccata gccgtaggtg cggtgtcgat attctcgctc ttgatttatc tactgcgaca
     4201 acttcgccta aaactcggcg acttgtctgc gctgccgagc gaaatgctgg caactgcaga
     4261 gattgtcaag aatgatatcc agacgaatgt ggatgcgtta ctgataaaat tcacggcgct
     4321 tcacgatgag atgcggctta gcttacacag tcggttcgac gcaattcaca agttgctgca
     4381 agcaactcac gaggtctcca accaagttgc cgacagtgaa atcgcggagg atgaggccag
     4441 ccaaaggccg ccacggatga cgagggtgca gtatgctcga gcgatcgcga ccaaggtaac
     4501 cgagcagtgg ctatccgggc gaactctacg atcactcgca gacagccctc atgtctttga
     4561 atttgagggg gcattggcga acggccaaca ttttaagttg aggttcggca ctccgtatcg
     4621 ccagggcatt ggtgtcgatg gtagattgcc gttcacactc gacgtttggg tcgacgggta
     4681 caagcacctg aactacgaat gggataccga cgggaactac gcccttaggg gattcaagaa
     4741 aggcagtgga gaatggctcg tagaattggc cgcatggggc ctcaaatctg aatcagtcgc
     4801 gaaggccgca tgacgtgcaa ggcctaactc ggcggattga gggcctgatt cccgagaagc
     4861 ctcggcgtct tagcctcact tcatcagcgc cttgaacttt cgcacttgcg agtctgcgtc
     4921 ctcctggtag gcgatttcgc cggcgcggcg tccatcgcca ccgacgagat acatcgatgt
     4981 cgagtgatga taggtatggc catgcttggt cggcacacgc tcgtagtagg cgccgaaccg
     5041 cgaggcgact gccgcgatct ccgcggggtg accagtgagc ccgatcaccc tcttgtcgaa
     5101 gtgcgaaaga tactccttga gtgcagctgg cgtatcgttc tcggcgtcga ctgagacgaa
     5161 caagaaccca attcgatcgg catcgggccc gagccgctcc agcaccccca tcaggtcgtt
     5221 gagtgtggtc gggcagactt ccggacagtt cgtgaaaccg aagaagatcg caaacggccg
     5281 ccccgcaagg cgctcgcttc ggaacggctg gccgtcagtg ccgatgagct cgaacggccc
     5341 gccatgcggc tcagccgccg cagcggttcc gcatggcatg ccgatggcgg tcgcgatggc
     5401 ggctccgagc agcagcgaaa gtcggcgtcg atcgacttcg tagtgcacgg cactactccc
     5461 tatttcttgt ccatcggtcc cgagcccttc ggcgcagcat gcggtccgct cgtctgcgtc
     5521 ccgagcggct cgatcagata ctcgacctcg atcgcgccgg ccttcgcgaa caccagcgtc
     5581 cccttgatcc tctctccgga cttcagcggc tgcttcagct ccatgaacat gacgtggaag
     5641 gagcccggac gcagaagaac actgccgccc ggtggaatct cgagaccgcc ggcgagttcg
     5701 ttcatgacca tgcggccgtc cttcatggcc atctcatgca cctcgaggcg tgcggcatgg
     5761 atgaagctgc caccaatgag gcgatcagcc tcctttcctg tattggtgat ccgcatgaag
     5821 ccgccggcca ccttgaagcc ttctggcgcc acccgcgacc agggatggtc gatgcggatg
     5881 gggccaatcg tgtaggtctg ttgcgccagt gcggcgacag acgcgagcgc caagaccgca
     5941 agcgccatcg ccgtggaagg aagggttctg gctggtttca tctctgtctc cgttgcgcta
     6001 tccgaggtca attcaatgct ttggcggcga caccatcgtc gcgtcttcga ccagcacctc
     6061 gacctcgatc cggcctgccc tctcaaactc gagggtcatg gcaaatgtat cgaaggcgga
     6121 gagcggcttt ttgatctgct tcaggatcag gcgcgggccg gtcgtcgtca tctcgagccg
     6181 gccccttgcc ccaatctcga atgctgaaac acgcttcgtt cgatgcgtgc cggcaacgtt
     6241 gtcgaggacg tgtagctcga ccgacccggc aatcggcgaa ctcgcgcctt tgagccgatc
     6301 gccttttgga gccgccacct tgacgaccat gtagacggcc gcgtcgtcac ccacgtttgc
     6361 ccccgcatga gtccagggat gcacgatctc gaccgctccc cgcttgacgc catgggcttg
     6421 caccggcccc agcagcaagg cggtcgtcaa accaatggcg agcaggcgcc gagaatcgat
     6481 catggtgatg tcttcatgcg ggcactcgat cgtcgtggct gctcgatgcc gaggcaagcg
     6541 cacgcacgca cgaaggccgg gcgatcaggt gtcggggtta cacttgggcg atggtcgcgg
     6601 catacggcgg cgcgcgggcg ccgaggccgc tcggtcgccc ctcggtccca ggtaaggcgt
     6661 acgccaccgc agggcgccat cccgctcgct gagcccattc gatcgcaatg gtcgaggcga
     6721 ccagcaccgc cggagtggac tgggcgacaa ggcaggacaa acagtgcccg tacgattcgg
     6781 gcaggggcgt tttcccagct tcgcggctat cgctgtcttc caatgtacgg ccgtggctcg
     6841 cgcacatctc gcgctttccg agaatcggat cgaccgtcgg atcgtttgag gccgggcgca
     6901 tcgcgcagac acctgccgtc gcggcattca ccagaaaggc gaatgcggct aggagagcga
     6961 cagatcgata tcgttgccgc gtcagcatgc atcgagtttc gcatgaatgc gccccgttgc
     7021 aagcttgatt ttgtcgcatt cggcgggagc accagcctcg ctcataggca gtcggttccg
     7081 gcaaaagcgc ctctgagcgg cgcactgtcc ttgcatcctc aaccccaaac gcatcgtcgg
     7141 ctgccgttcg gtcggccggc cacgttattt tcctagcctc ccaaggggtc aggcccatga
     7201 cgctcaaatt tcatttcttc acgcgagaca cgatgccgga cgccgccgtg ctgcgatcgg
     7261 cggcccatgc caaccgcatg ctcgccgagc agctccccga cgatccgccg tatgacgcag
     7321 cggacgagct caggcgctgg catgcaatgc cgtcgcaggc gcgctcgcaa gtctggatcg
     7381 ccgaggcgaa cgggaatgtc gtcgccgagg caaacctcaa ttgggaggag ctcgagagca
     7441 atcgcgcgct tgcccacgtc tacgtctcgg tcgaacgcga ctggcgtggc caaggcctag
     7501 gcgcgaccct cctgtcactc gcggtcgagc gagcgatcga ggccggacgg cccaagctca
     7561 tgtcgatgag cacgagccgc gtaccggccg gcggacggtt cctcgatcgc gttggcttca
     7621 cccgccgcct cgagatgcac acgaaccagc tcaaggtcgc agatctcgat cgggcgctcg
     7681 ttgacgcatg gagccggccg gatcccgcgc gcacaggcga ctatgcgatc gaggtcttcg
     7741 atggccccgt gccggaccac cggctcgagg cctacgcgga gcttgtcggc gtgatgaaca
     7801 ccgcgccgcg cggcacgctc gaggtcgagg acacgaagat caccgcgagc atggtgcgcg
     7861 agtgggaggc ctccctcgtc gcgagcggct ggcggcggct cctggcggtc acgcgccacg
     7921 tgccgagcgg agtgctcgcc gggttcaccg agctgcggtg gacgccgacc cgcgcctcac
     7981 tcgtctggca gggcggtaca ggggtcgtcc cctcgcaccg caaccggggc ctcgggcgct
     8041 ggctcaaggc gcaaaacctc gcggcgctgc tctcggcgaa cgacaaggtg cgcttcgtgc
     8101 gcaccggcaa cgccgacagc aatgccccga tgctggcgat caaccaccag ctgggcttcc
     8161 gcccgttcac ggcgcgggcc ctctggcagg ggaacgccgc cgaggtgagc ggacagctcg
     8221 ctcggcagcg ccggcaggct gcatgatcgg cctgccggtc aatcggcgcg cccgcgaact
     8281 tgccgcagct gcctcatgag gtcgtgcggc gaggctgcgg gccgccgtcg ttgctgggag
     8341 cgaggtcaca tcgggctgcc gacgattgcc aacgcctccc gtcggcagaa gatctccggc
     8401 ggaatgttgc gcagcttacc gaggcggtcc tcccgctcct cggcgcgcgc aatgcagagt
     8461 tgctcgagcg cccgggtcgt cgtgtcgatg atgcgtgggc tggccctggc ggagaatgcc
     8521 gacacgggcc gcggccccac tgcggcgacg cgcaggtcct tcagaggaaa cgcgatgccg
     8581 atcgtcagac tgtgaatgtc ctggcgctcg ccggaaatca gggccgtggc attgataagc
     8641 ttaaaggtct tcggcagcgg cgagagcacc catttcccgt ccgccttcgc gtggatcctg
     8701 gtcgtgaagt tgattacgtc ggaaaaatcg cctttgccgc caccgagctc ggacagctcg
     8761 ataaaggtgt tcatcactct cgtcatgccg atcgagcccg tcagcgggta aaggccattt
     8821 gcgtccggtt gcacgtagct ccggcagtcg agacggacca gctcgcgaaa cgtctcgacg
     8881 gtcttgaact ttcggtgccc cgaccgctcg cggccgaggc cgccgtcggc accgagcgtg
     8941 aatgtgcgct tcggataggg gagctggaag gagagggagc cgctctggtc cgacttctcc
     9001 gtaatcttga aggagaaatc ataggcgatc gagctgtcgg catagttgag gaacttctcg
     9061 gcgagcagcg gatccgcctt cttgatccgg gcaaagtagt cggaccgctc gaggatcagt
     9121 tccggttcga tgccggcaaa ggccggattt cggtccagcg ccatcttgat gcgtgtccgc
     9181 acagagtcgc gcgcctcgca gcgcacgttc cggacgatgt cctcagtcgt atactgcgag
     9241 acgtcgtccg ggatggggtg aatggcacaa cctgaaagcg ccgtgaccgt ggcagccagg
     9301 aaaagcggtc tcaatctcat gatccctctt ttaaatatgc gctcgccgga atttcccgga
     9361 gcagccgaga gccttgactg attcgggtgc tcctcgcctg tggccgagat ggcttctcat
     9421 cgctttcatc gatcgggtga cttgagcggt cttttcgatt gacaggccgc gttggccgca
     9481 taaacggccg gcatgcaaca cacacgcttc atcaccgaca acacgcgcct catggcgccg
     9541 ccgctcgtcc ccgagatccg cctgcat
//
LOCUS       DAORNH010000172         9533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5302_length_9533_cov_2.353556, whole genome shotgun sequence.
ACCESSION   DAORNH010000172 DAORNH010000000
VERSION     DAORNH010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5302_length_9533_cov_2.353556"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK264_16220"
     CDS             <1..440
                     /locus_tag="PK264_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRD77453.1"
                     /translation="VANLVPPYETTGKYHGVSAALEFATLNLRVRHIVVMGHSACGGI
                     RAALEANAARQSDARFISGWMSILDKARDSVLEGAKDRPIADLRADLERAGIRASIDN
                     LRTFPCIQILEAKGRLSLHGAHFDIASGQLYMLNQATGGFVPA"
     gene            479..664
                     /locus_tag="PK264_16225"
     CDS             479..664
                     /locus_tag="PK264_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HRD77454.1"
                     /translation="MAAVSSIAVAVVFAVLVGGLIVMARGKSANASQLFMRWRVGLQF
                     LAIVLIILTAYLMRRTT"
     gene            686..1720
                     /locus_tag="PK264_16230"
     CDS             686..1720
                     /locus_tag="PK264_16230"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRD77455.1"
                     /translation="MLHSGLKAVLAMAASVVFLIGSNMPASAQKGAAAPPPCRGTDYI
                     DSLEKSDPAAYKRLRTLAGEIPNSNAVLWKIEGKGVVPSHLFGTVHVTDDRIHAMPKA
                     AQDALAAAKTVALEVDDLSPVKMAAAFAKLNNLIVAPDGKSLAKQLDESELKHARSVL
                     GRVGMPGEMINMVRPWLVAVMMAMPECEVKRMGAGLQPLDSSIQDKAKANKAQVVGLE
                     TIEDQLRAMASVPDETQIIMIKATLKLHNRLDDLFETTLSRYLKRDLGAIWALQIILT
                     EKLGHPRSSLEAFEKSLIVVRNRKMRDGALPLLAKGNAFIAVGALHLPGKEGLVELLR
                     AEGYTVAKVD"
     gene            1942..3096
                     /locus_tag="PK264_16235"
     CDS             1942..3096
                     /locus_tag="PK264_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HRD77456.1"
                     /translation="MGHSPASPECPGRASVRLIASDTTWIEGAARAQLDTAARLAGMV
                     AVVGFPDLHPGKGTPVGAAFVSRGCIHPTLIGNDIGCAMSFWATDVSARRLKLDRWVK
                     RLPDLELPWDGDLAAWCEPFGLGPSPSDVGLGTIGGGNHFAELQIVVEVRDEVRFASA
                     RLDKDFACLLVHSGSRGLGQAIWREVLAEHGANPLEAGSEAAVRYLARHETARAFARA
                     NRALIAKRFGDGIGAEVRCIADIEHNVVTRETWRGGEAWVHRKGAAHAHTDLVVIPGS
                     RGTPSYLVAPTGDLETAAFSLAHGAGRRWQRGYAKSRLSHRYRVSDLERTEYGGRVVC
                     EDRELIYDEAPQAYKDIAGVIGDLEAAGLIAVVAELRPVLTYKVRRRSDD"
     gene            3089..>3343
                     /locus_tag="PK264_16240"
     CDS             3089..>3343
                     /locus_tag="PK264_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HRD77457.1"
                     /translation="MTDPSMSGEVVWLQITSGRGPVECQLAVARLAPVIVRAAERAGL
                     AAKIIEAVQAAEKGTLLSALVKVSGDGAEDFARTWAGSIKW"
     assembly_gap    3344..3369
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3370..3741
                     /locus_tag="PK264_16245"
     CDS             <3370..3741
                     /locus_tag="PK264_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRD77458.1"
                     /translation="KRKNWFVGVDLVGMPSGRGSRRIDARDVTFSTMRAGGAGGQHVN
                     KTESAVRALHRPSGLVAEAREERSQHMNRRLALARLADLLARGVAAAAAEADRSRWTE
                     HDRLERGNPTRTFQGPDFDPA"
     assembly_gap    3750..3826
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3827..4369)
                     /locus_tag="PK264_16250"
     CDS             complement(<3827..4369)
                     /locus_tag="PK264_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77459.1"
                     /translation="MLRPFVAFIAPVLLGSATLAGLAPEALARSDSVPPRGPNGPPSA
                     GPVKGATTAPDFRGIRGHVLVESIPAGYRRYARRTNLAYKGGEQIVLYGEPIGFGWSA
                     VVNGQQLHMVVNIEIVDQTGAVVFKPEDVTLRQTEATMPAEPFVHVAFVLPTLKPGEY
                     AVHYKFHDRANKKMVRFHRSI"
     gene            4554..6143
                     /locus_tag="PK264_16255"
     CDS             4554..6143
                     /locus_tag="PK264_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HRD77460.1"
                     /translation="MLMTARGRRGMVTSPHQLASSAGIAILREGGNAIEASIGMAAAL
                     AVVYPHMTSIGGDGFWLIAPPGKDPIGIDACGAAVAAARPELYKANGHATIPWRGPLA
                     ANTVAGTTSGWGAALALSQELGGGLPLSRLVEDAAWHARNGFPVTASQAALTSEKLPE
                     LAAAPGFASTFLIDGRLPREGETMRLAALGATLEQLGRDGTESFYRGPLARSLAANLS
                     AAGSPVTSADLSAHRALRVNPLSVSLAGARLFNMPPPTQGLASLMILSLYERLGIRES
                     DRFDHIHGLVEATKQAFIVRDRIIGDPASMTEDPRRFLEPQLLDGLAARIDRRRALPW
                     PAPVDKGDTVWLGAIDGSGLAVSFIQSIYFEFGSGVVLPDAGIVWQNRGSSFLLEGRG
                     PRALAPGRKPFHTLNPALARMSDGRVMVYGTMGGEGQPQTQSAIYSRYAIHGGSLQAA
                     ISAPRWLLGKTWGANSVTLKLENRFDPQLVSALRDTGHDVELLQGYSSTMGHAGAVVR
                     HLDGSFEGAGDPRSDGCALGW"
     gene            complement(6169..7068)
                     /locus_tag="PK264_16260"
     CDS             complement(6169..7068)
                     /locus_tag="PK264_16260"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD77461.1"
                     /translation="MAIDAVTASAEARRFRSPQAALSYGVGAARGGNISEAIPALEEA
                     AAAGLLEGQYFLAEIYADNRGNFTDHRKSFSLFQRIVEEHAGSVEADEKPESTYLAKS
                     LVALAGYWLRGLPEMNVRPTPERAAYYLDMAANLFGDEDAQFELAKLYLVGEGVPKDL
                     RLGLHYLSTLSSTNRHAGAQAFLADLYWRGKFVEKDQLRAFALIMTAVENASPADELW
                     IDGIFATIYCGVPSDVRKQSDSYVAEFKRRYTPRVQPDRNGSASLTASRVCENGDPVP
                     MLRKAPTVYTRPGVGMLPTGDGR"
     gene            complement(7523..7741)
                     /locus_tag="PK264_16265"
     CDS             complement(7523..7741)
                     /locus_tag="PK264_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRD77462.1"
                     /translation="MARDDGGAPGQAAGDMSENEARAILGVEETATDDQIRKVHRELM
                     QKLHPDRGGSNYLAAKINEAKARLLKQR"
     gene            7760..8263
                     /locus_tag="PK264_16270"
     CDS             7760..8263
                     /locus_tag="PK264_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77463.1"
                     /translation="MLPIQETLDDRGGCLVGQPAVMPEMREFYRPQALDPASSEEAFE
                     DAAAHATIGAADRNIEMVGGPALRSTTNTAERQGAAELAQQPEGTADRCEREANLSTR
                     REIESSQTGAGKPRDPQFASNFRDTCPPIPPERDQPDRRNSEQDEDRLHRSALFSAWL
                     SHPRPYR"
     gene            complement(8226..8834)
                     /locus_tag="PK264_16275"
     CDS             complement(8226..8834)
                     /locus_tag="PK264_16275"
                     /inference="COORDINATES: protein motif:HMM:NF022155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HRD77464.1"
                     /translation="MDLGAIVPQREVATQSPRESVGPAPEPVIRIVDDAAVPLDPLKV
                     QPKAIVAPGLPKLGEPSLEASSTACSAHLDATDATSAAAVAAAAATSAVVSAMRTVPS
                     DPAPITVSAAPIKVPTADDSPPPVAASVAAPPAPAIGAASMVGSADALAPVRTLEDTV
                     SDLLRPMIRQWLDQNMPRMVERALKIEVAGPSDTDAGGSAKR"
     assembly_gap    9167..9301
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1580 a         3095 c         3084 g         1536 t
ORIGIN      
        1 acgtcgccaa tctggtcccg ccctacgaga cgaccggcaa gtatcacggc gtcagcgccg
       61 cgctcgagtt cgcgacgctc aatctgcgcg tgcgccacat cgtcgtcatg ggacactcgg
      121 cctgtggcgg catcagggcg gcgctcgagg ccaatgcagc gcgccagtcg gatgcccgct
      181 tcatctcggg ctggatgtcg atcctcgaca aggcgcgcga cagcgtgctc gagggtgcca
      241 aggaccgccc gatcgccgat ctcagagcgg acctcgagcg cgccggcatc agggcatcga
      301 tcgacaacct gcgtacattc ccctgcatac agatcctcga ggcgaagggg cgtctctcgc
      361 tgcacggcgc gcacttcgac atcgcatcgg gacagctcta tatgttgaat caggcgaccg
      421 gcggtttcgt acccgcgtaa acccgccgcc cgccaggacg agctttggag cagagcgcat
      481 ggccgcagtg tcttcgatcg cagtcgccgt cgtctttgcc gtgctggtcg gcggcctcat
      541 cgtcatggcg cgcggcaaga gtgccaatgc ctcgcagctc ttcatgcgct ggcgagtcgg
      601 cctgcagttc ctcgccattg tcctgatcat actgacagct tatctcatgc gtcggacgac
      661 ctgaggaccc taaggagcgg cggcaatgtt gcattccgga ttgaaggcgg tgctggcaat
      721 ggccgcatcg gtcgtctttc tgatcggttc gaacatgccg gcctcggcgc agaagggggc
      781 ggcggcaccg ccgccctgcc gtggtaccga ctacatcgac tcactcgaaa agtccgatcc
      841 ggccgcctac aagaggctcc gcacccttgc aggcgagatc ccgaactcga acgccgtcct
      901 ctggaagatc gagggtaagg gggtagtgcc ctcgcacctc ttcggcacgg tgcacgtcac
      961 cgacgatcgg atccacgcga tgccgaaggc ggcgcaggac gcgctcgccg ccgccaagac
     1021 ggtcgccctc gaggtcgacg atctctcccc ggtgaagatg gccgcggcgt tcgccaagct
     1081 caacaacctg atcgtcgccc cagacggcaa atcactcgcc aagcagctcg acgagagcga
     1141 attgaagcat gcccgctcgg tgctcggccg cgtcggcatg ccgggcgaga tgatcaacat
     1201 ggtgcggccg tggctcgtcg ccgtgatgat ggcgatgccc gaatgcgagg tgaagcggat
     1261 gggcgcgggc cttcagccgc tcgacagtag cattcaggac aaggcgaagg cgaacaaggc
     1321 gcaggtcgtc ggcctcgaga cgatcgagga ccagctgcgt gccatggctt cggtgccgga
     1381 cgagacgcag atcatcatga tcaaggcgac tctcaagctg cacaaccggc tcgacgacct
     1441 gttcgagacc acgctgtcgc gctacctgaa gcgcgacctt ggtgccatct gggccctgca
     1501 gatcatcctc accgagaaac tcgggcaccc gagatcctca ctcgaggcct tcgagaagag
     1561 cctgatcgtc gtgcgcaatc gcaagatgcg tgacggcgcc ctgccgctgc tcgccaaggg
     1621 taatgcgttc atcgcggtcg gcgcgctgca tcttcccggc aaggaagggc ttgtcgaact
     1681 gctgcgggca gaaggctata cggtcgcaaa ggtcgattga accgtgcacc gggacgccgc
     1741 ggtgaggcag acatggggcg tcggcgtacc gctgccggat tgcgctctgg ccggagcatt
     1801 cccatatcga ttgaacgaca atcgaatctg gagaccagta catgcagcca acgacacgcc
     1861 acacgacccg agcaacaatt ccgttctcgc cgcgtcctgt gagcctgtct cgctgcgcgg
     1921 ccaatccagg tacgagcaat catgggccat tcgcccgcct cccctgaatg ccccggacgc
     1981 gcgagcgtcc gcctcattgc ctccgatacg acatggatcg aaggcgctgc gcgcgcccag
     2041 ctcgatacgg ccgcccgcct agccggcatg gtggccgtgg tcggcttccc tgacctgcat
     2101 ccaggtaaag gcacacccgt cggagcggca ttcgtctcgc gcggttgcat ccacccgacg
     2161 ctgatcggca acgatatcgg ctgcgccatg agcttctggg cgaccgacgt gtcggcgcgc
     2221 aggctcaagc tcgaccgctg ggtgaagcgc ctcccggatc tcgagctgcc ctgggacggt
     2281 gacctcgccg cctggtgcga gccgttcggg ctcgggccat ccccgagcga cgtcggtctc
     2341 ggcacgatcg gcggtggcaa tcacttcgcc gagctgcaga tcgtcgtcga agtccgcgac
     2401 gaggtccgat tcgcctcggc gcgcctcgac aaggatttcg cctgcctgct cgtacactct
     2461 ggaagccgcg gcctcggcca ggcgatctgg cgcgaggtgc tcgcagagca cggcgcaaac
     2521 ccgctcgagg ccggcagcga ggcggcagtg cgctacctcg cccgccacga gaccgcgcga
     2581 gcattcgcac gggccaatcg cgccctgatc gccaagcgat tcggtgacgg catcggcgcc
     2641 gaggtccgct gcattgccga tatcgagcac aatgtcgtga cgcgggaaac ctggcgcggc
     2701 ggcgaggcct gggtccaccg caagggtgcc gcgcacgcgc acaccgacct cgtggtgatc
     2761 ccgggctcgc gcggcacgcc gagctacctc gtagcgccga ccggtgatct cgagacggca
     2821 gccttctcgc tggcccacgg tgccggccgg cgctggcagc gaggctacgc caagtcgcgg
     2881 ctgtcgcatc gctatcgagt gagcgacctc gagcggaccg agtatggcgg ccgtgtcgtc
     2941 tgcgaggatc gggaactcat ctacgacgag gcgccgcagg cctacaagga catcgcgggc
     3001 gtgatcggcg atctcgaggc cgcgggtcta atcgcggtgg tggcggagct acgccccgtg
     3061 ctcacctaca aagtcaggag gcggagcgat gactgatccg tcgatgtcgg gcgaggtcgt
     3121 gtggctgcag atcacttcgg gccgcggccc cgtcgagtgc cagctcgcgg tggcaaggct
     3181 cgcgcctgtg atcgtgcggg cggcggagcg ggccggactg gccgccaaaa tcatcgaggc
     3241 ggtccaggct gctgaaaagg gcacacttct ctccgcgctc gtcaaggtct ccggtgatgg
     3301 cgccgaggac ttcgcccgga cctgggcggg cagcatcaag tggnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnna agcgcaagaa ctggttcgtc ggcgtcgatc tcgtgggcat gccgtcgggt
     3421 cggggcagcc ggcgcatcga cgcgcgcgac gtgacgttct cgaccatgcg ggccgggggc
     3481 gccggcggac agcacgtcaa caagacggaa agcgccgtac gcgcgctgca ccgtccgagc
     3541 ggcctcgtcg ccgaggcgcg tgaggagcga tcgcagcaca tgaatcggcg gctcgctctc
     3601 gcacggctcg ccgatctcct ggccagggga gtggcggcgg cagccgccga ggccgatcgc
     3661 agccggtgga ccgagcacga ccggctggag cgcggcaacc cgacgcgaac gttccaagga
     3721 ccggatttcg atcccgcgtg acgacggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngatg ctgcgatgga
     3841 aacgcaccat cttcttgttg gcgcggtcgt ggaacttgta gtgcacggca tattcgcctg
     3901 gcttcagcgt cggcagcacg aaggccacgt gcacgaacgg ctcggccggc atcgtcgcct
     3961 cggtctggcg cagggtcacg tcctctggct tgaagacgac cgcacctgtc tgatcgacaa
     4021 tctcgatgtt gacaaccatg tgcagctgct gcccgttgac gacggccgac cagccgaagc
     4081 cgatcggctc gccgtagaga acgatctgct cgccaccctt gtaggcgagg ttggtgcggc
     4141 gcgcgtagcg acggtacccg gctgggatgc tttcgacgag gacgtggccg cggatgccgc
     4201 ggaagtcggg agcggtggtc gcacccttga cgggccccgc cgatggcgga ccgttgggtc
     4261 cccgcggcgg cacgctgtcg ctgcgcgcca gtgcctcggg agccagaccc gcgagcgtgg
     4321 ccgatccgag aagcacagga gcaatgaatg caacgaatgg acgcaacata gccgacctca
     4381 atggttgaga acgacgcgac gcggagcgcc atgcgacgaa ccgcgctcca gactgatcct
     4441 atcacgtcac ttgtcatgcg cagagcctga ttgcgaacgc gctttgccgg cagccccggg
     4501 actgctctaa gactgggctg atcacatcgc caatcgcaac gacggacagc cagatgctca
     4561 tgaccgcgcg cggccgcaga ggcatggtga cctcgccgca ccagctcgcc agctcggccg
     4621 gcattgccat actgcgcgag ggcggcaatg ccatcgaggc gtcgatcggc atggccgcgg
     4681 cgcttgcggt cgtctatccg cacatgacct ccatcggcgg cgacggcttc tggctgatcg
     4741 cgccgcccgg caaggatccg atcgggatcg atgcctgcgg cgcggctgtc gccgcagcga
     4801 gaccggagct ctacaaggcc aatggccacg cgacgatccc gtggcgcggg ccgctcgccg
     4861 ccaacactgt ggccggcacg acctccggtt ggggcgccgc gctcgcgctt tcgcaggagc
     4921 tcggcggcgg cctgccgttg tctcgcctcg tcgaggatgc cgcgtggcat gcccgcaatg
     4981 gcttccccgt gacggcgagc caggcagccc tcacgtccga gaagctcccc gagcttgcgg
     5041 cggcgccggg ctttgcctcg acgttcctga tcgacggtcg cctcccccgt gagggcgaga
     5101 cgatgcggct cgccgcgctc ggtgccacac tcgagcaact cggcagggac gggacggaga
     5161 gcttctaccg cgggccgctc gctcgcagtc tcgcggctaa cctttcggcg gcaggcagtc
     5221 ctgtcacgtc agccgacctt tctgcgcatc gggctctgcg agtgaatccg ctgtcggtct
     5281 cgctcgccgg cgcgcgcctc ttcaacatgc cgccgccgac gcaggggctc gcctcgctaa
     5341 tgattctttc actgtacgaa cgccttggca ttcgggagtc cgatcgtttc gaccatatcc
     5401 acggtctcgt cgaggcgacg aagcaggcct ttatcgtgcg cgatcgcatc attggcgatc
     5461 cagcctcgat gaccgaggat ccgcgccgct ttctcgagcc tcagctcctc gacggtctcg
     5521 ctgcacgcat cgataggagg cgcgcgctcc cctggccggc gccggtcgac aagggcgata
     5581 cagtctggct cggcgccatc gacgggagcg ggctcgccgt gagcttcatt caaagcatct
     5641 atttcgagtt cggctccggc gtggtgctgc ccgatgccgg tattgtctgg cagaaccgcg
     5701 gctcgagttt tctcctcgag ggccgcggtc cgcgcgctct ggcaccgggc cgtaagcctt
     5761 tccacacact caaccctgcg ctcgcccgca tgagcgacgg ccgcgtgatg gtctacggca
     5821 cgatgggcgg agagggacag cctcagacgc agtcggccat ctactcgcgc tacgccattc
     5881 acggcggctc gctccaggcg gcgatctctg cgccgcgttg gctgctcgga aaaacgtggg
     5941 gggccaacag cgtgacgctg aagctcgaga accgcttcga tccccagctg gtcagcgcct
     6001 tgagagacac cggacacgac gtcgagctgc tgcagggcta ctcgagcaca atgggccatg
     6061 ctggtgccgt cgtgcgccac ctcgacggca gcttcgaggg agcaggtgac ccacgcagcg
     6121 acggctgtgc gcttggctgg tgagcctgat catgctgacc ggtcgcggtc agcgcccgtc
     6181 gccggtcggc agcatgccga cgcccggacg ggtatatacg gtcggtgcct tgcgcagcat
     6241 cgggaccggg tcgccattct cacagacgcg cgaggcggtg aggctggccg agccgttgcg
     6301 gtcgggctgc acgcgcggcg tatagcgccg cttgaactcg gcgacatagg aatccgactg
     6361 cttgcgcacg tcgctcggca cgccgcaata gattgtcgcg aagatgccgt cgatccacag
     6421 ctcatcggcg ggtgacgcat tctcgaccgc cgtcatgatc agcgcaaagg ccctgagctg
     6481 gtccttctcg acgaacttgc cgcgccagta gagatcggca aggaaggcct gggcgcccgc
     6541 atgtcgattg gtggaactca gcgtcgagag atagtggagg ccgagccgga ggtccttcgg
     6601 cacgccctcg cccaccagat agagcttggc gagctcgaac tgggcgtctt cgtcgccgaa
     6661 cagattggcc gccatgtcga ggtagtaggc ggcacgctcc ggtgtcggac gaacgttcat
     6721 ctcggggagc ccgcgcaacc aatatccggc gagtgccacc agcgacttgg cgaggtaagt
     6781 ggattccggc ttctcgtcgg cctcgacact gcccgcgtgt tcttcgacga tacgctggaa
     6841 caggctgaac gacttgcgat gatcggtaaa gttaccgcga ttgtccgcat agatctcggc
     6901 gaggaagtac tgcccttcga gcaggccggc cgcagccgcc tcctccagag ccggaatggc
     6961 ctcgctgata ttgccgccgc gagccgcgcc gacaccgtag ctcaatgcag cttgcgggga
     7021 tcggaaccgg cgcgcttcgg cgctggcggt gaccgcatcg atcgccatgc cgccaatcga
     7081 aatggcaagc gccataatcg ccaatcgcgc catccgtcga gcggaaccgg gcacgagtcg
     7141 atcgttcgac tccggactat cgacaacctt cgtcatgtcg ctttcgccac gtctttcatg
     7201 cgcgccgcgc acatcgtcgc cgcgatgtgc cggagcagcc cccgcccgaa tccaaaccgg
     7261 cccctgaccc aacacccgtc cagttccgcc aacgaaatgt gccaaccctt tatggctcaa
     7321 aggtggcgcg cctgccccag tatgtggcgg ctacgcaaca atcgagcatc aggtggtcgg
     7381 caggatctcg gtgccgcggg tgacgacgcc atgcgaaatc cgacaattga ataggcgacc
     7441 aaggcggcgc cagcgtgtcc gaccaaggaa attcatgagg cgtcggagga gaatcgcagc
     7501 ctgtggagaa cgcctgcgac cttcagcgct gcttcagcag acgcgccttg gcctcgttga
     7561 tcttcgccgc aaggtagttc gagccgccgc gatcgggatg cagcttctgc ataagctcgc
     7621 gatgcacctt tcggatctgg tcgtctgtcg cggtctcttc gacaccgagg atggcccgcg
     7681 cttcgttctc ggacatgtca ccggcagcct gtcccggggc gcctccatcg tcgcgcgcca
     7741 tgtcctcccg ccaggtcgga tgctgccgat ccaggagacc ctcgacgatc gcggcggatg
     7801 cctggtcggt cagccggcag tcatgccaga gatgcgcgag ttctaccgcc ctcaggctct
     7861 cgatccggcg tccagcgaag aagcctttga ggatgcggcc gcgcacgcga ccatcggtgc
     7921 ggctgatcgc aacatcgaga tggtcggtgg tccggcgctc cgaagcacca ccaacaccgc
     7981 cgaacggcaa ggagctgccg agctcgcgca gcaaccagag ggcaccgccg accgctgcga
     8041 gcgcgaagcc aatctgtcca cgcgccgcga aatagagagt agccagacag gcgccggcaa
     8101 gccccgcgat ccgcagtttg cgagcaattt ccgcgacact tgtccgccga taccacccga
     8161 gcgcgatcaa cccgaccgcc gcaacagtga gcaggatgag gatcgattgc atcggtcagc
     8221 gcttttcagc gcttggctga gccacccgcg tccgtatcgc taggtccggc aacctcgatc
     8281 ttcagggcgc gctcgaccat gcggggcatg ttctgatcga gccactggcg gatcatgggc
     8341 cggagcaggt cggataccgt gtcctcgagt gtccgcacgg gcgccaacgc gtcggccgac
     8401 ccgaccatcg aggccgcgcc gatggctggt gctggcggtg ccgcgaccga ggctgcaacg
     8461 ggcggcgggg aatcgtccgc tgtcggaact ttgatgggcg cggcagacac cgtgatcggc
     8521 gccggatcgg agggtactgt gcgcatggcg gacacgactg ccgaagtcgc ggcagcagcg
     8581 gccacagcag ctgccgatgt tgcatccgtc gcgtcgagat gagcgctaca agccgtgctc
     8641 gaagcctcga ggctcggctc accgagcttc ggcagtccgg gcgcgacgat ggccttgggc
     8701 tgaaccttga gcgggtcgag cggcaccgcc gcatcgtcga ctatgcggat cacgggctca
     8761 ggagccggcc cgaccgactc gcggggcgac tgcgttgcga cttccctttg cggcacgatt
     8821 gcaccgaggt ccatgacagg cgcacccgga cccgcctcag gcgccgctcg tgacggagca
     8881 ctctcgaaaa gtggaggtac cggccgacgc gctgtttcgg tatcagtgcg cgcagggtcg
     8941 agatcgaggg cgttcacagc gggcagagtc ggtccggaaa aaagagttct cggaattgtg
     9001 ccgacggcga ccggtccgct ccccgcggct cccgctgcat cgcgtgcagc ctcagcaacg
     9061 gtgacgcgcg gttccgcagc gccgatcggc ggcgtttgcg gttggctgac tggcgccgcc
     9121 ggcctcacgc tgccgaagtc cgcgactgtc gatggagccg gattcgnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 ngagagcggt gccgataggt ccttcgccgg cattccggaa ggggcgggag cccccgggcg
     9361 cggcatcatg gggctcgcac tcgctgcatg accgttgctg cgcccggcgg ctggcagagt
     9421 cgagcctaga aatcctggcc gttcgagagc ctccggcgtc ttgtcaggcg tagctggcgc
     9481 agtgggcctg gcaggttttg ccactgcttt cggcgtcata gctggttggc gcg
//
LOCUS       DAORNH010000173         9449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5367_length_9449_cov_2.584628, whole genome shotgun sequence.
ACCESSION   DAORNH010000173 DAORNH010000000
VERSION     DAORNH010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9449
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_9449_cov_2.584628"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK264_16280"
     CDS             complement(<1..712)
                     /locus_tag="PK264_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HRD77465.1"
                     /translation="MSLTRRTLLGGLSTVAATAVGGRHLFAQGETIKVGVLHSLSGTM
                     AISETTLRDVMLMLIEEQNKKGGLLGRKLEAVVVDPASNWPLFAEKARELISQKKVAS
                     VFGCWTSVSRKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDYLM
                     SKDGGDVKRWVLAGTDYVYPRTTNKILEAYLKAKGVAPADIMINYTPFGHSDWQTIVA
                     EIKKFGSAGKKTAVVSTIN"
     gene            complement(733..990)
                     /locus_tag="PK264_16285"
     CDS             complement(733..990)
                     /locus_tag="PK264_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77466.1"
                     /translation="MSVYPKVETGPELHVFPAAAQENVSSRVSNDFSAAAASKQGSLH
                     SPVAGIRSTVIENYLVESKAKHRILCAATLLAQALLIVNQS"
     gene            1235..1933
                     /locus_tag="PK264_16290"
     CDS             1235..1933
                     /locus_tag="PK264_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77467.1"
                     /translation="MRVRADSRVVELEYRFGAGSERLYRNFRLMLGQLGDAGTVEFGP
                     GNKFYLVAADKGAAVPPPPSGGPSPAPSGAKIATKRLLVVVDDADRTSTHASNRSMRM
                     LLDAVSTGLTERSFEVISPMGVDVGTRVKQPTKAALEVTAAIPGGARILLVKLTGDGK
                     RLGPGGAASPLVARAVLRLADATDAVLVWEGSADAQLEHSCARDANCLVDAHGRLARS
                     ILASGWAHIAAKLK"
     gene            complement(1934..2554)
                     /gene="ruvA"
                     /locus_tag="PK264_16295"
     CDS             complement(1934..2554)
                     /gene="ruvA"
                     /locus_tag="PK264_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRD77468.1"
                     /translation="MIGKLRGKVDAVGEAHAVIDVNGVGYEVNCSARTLRSLKPGDDA
                     TLSIETHVREDAIRLFGFTSEVERSWFRTLQSVQGVGAKVALSVLSVLSPQDLASAIA
                     LQNWGAVAEANGVGKKLAQRITAELRDKAPGLSMLGIGPTAAGATMPTPMPVASHAVA
                     EAISGLVNLGYQPAQAAQAVAAAQRDLGEEADTAKLLRRGLKELAR"
     gene            complement(2664..3800)
                     /locus_tag="PK264_16300"
     CDS             complement(2664..3800)
                     /locus_tag="PK264_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HRD77469.1"
                     /translation="MASMKGTVGDDRIFGSTLQDQIMADAGNDVVTSDGGDDAVYGQD
                     GDDRLFGDAGNDAIYGGLGRDVLDGGEGNDLLRGGADDDSLRGGSGSDTLYGDTGNDS
                     IWGEAGNDVIFGGAGNDVLDGFGDDDTLTGGTENDTLRGGDGNDSLAGDSGDDRLEGG
                     NGDDALRGSTGNDLLMDDAGMDMVDGGSGNDTLVAGGGDDYLDGGSGFDTLDLSGAGA
                     VVVDLNAHTAFGDLTGADAVWGIERVVGSSGDDMLRGDQSGNIFVGGSGNDLFRGAAG
                     VDSYTGGAGNDTYTFLMKDVLVGGVHQGVDVISDFNTGDTLDLRDFVKSYVPTLENSI
                     RMTSTVEGTMVSANVDGQFVDIVMLSGVRGGDADAWASDYMLMA"
     gene            4145..4951
                     /locus_tag="PK264_16305"
     CDS             4145..4951
                     /locus_tag="PK264_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HRD77470.1"
                     /translation="MPAPDAPVLYEQDGGIVTVTLNAPKTRNALDDPVIDGIVEACRR
                     INADMSVRCAIITAKGEAFSSGGNVKAMRNGEGMFGGTPVDMRRGYQTTIHGIPRSIY
                     ALEVPTICAVNGPAIGAGCDLTLMCDIRIAGRKAQFAESFLRVGLVSGDGGAWFLPRV
                     VGLSRAYEMTLTGDVVDAERAERIGLVSKVVDDDMLMDEARALAARIAAHPPHSIRLN
                     KRLLRESQGVSLDVALELASAMQALVQSTSDQKEAVAALIEKRKPSYKGK"
     gene            4954..6183
                     /locus_tag="PK264_16310"
     CDS             4954..6183
                     /locus_tag="PK264_16310"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD77471.1"
                     /translation="MAGALEGVRIIDMTAVVLGPVATLHLADMGADVVKIEPPEGDVM
                     RNAGHSPVRKMGPIFLNANRNKRSLALDLKKPAAVDVVKRLVAKADVFVHNNRPAAVE
                     RLGLGYDDLKVVNPRLIYAFAHGYKRTGPYGHMPAYDDLVQAASGAAMLQSRIDGGEP
                     RFLPTLVADKTTGLHLGMAILAALYARERTGIAQQIEVPMLESVTAFWMLEHLFDHTF
                     VPPRGEMGYARILNEFRRPYRTRDGFVMALPYTAKHWSAFTDGTGRKDLAIDPRFVTL
                     EARANNQAAAMAAIAEIMLTRTTGDWLAFCEKNDIPAQRINDLEDLPTDPHLAATSFF
                     TRREHPTVGPVLTMASPFNFSETPTSFRRHAPTLGGDGAEVLTEAGFSESEIGALRSE
                     GALVEDAGRPRDAAPSS"
     gene            6248..6775
                     /locus_tag="PK264_16315"
     CDS             6248..6775
                     /locus_tag="PK264_16315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRD77472.1"
                     /translation="MTTRSMSPEAQSRPVNAGDLATISGLHARVFGPGRFVRTAYRVR
                     DGLPLVSPFCRVIVLGGEIIAALRFAPVTVGGVGRALMLGPAAVTPEHANLGYARRLI
                     AEGHAAARDAGIRLVLLVGDEPYYGRLGFVRVPKGQMSMPGPVNPDRLLALELEAGAL
                     ASYRGLIVGDAAATP"
     gene            6772..7449
                     /locus_tag="PK264_16320"
     CDS             6772..7449
                     /locus_tag="PK264_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRD77473.1"
                     /translation="MSPSVVAPSASCDPGRDCPGCERLVAFRTKHRATEPTWHNAPVA
                     SFGPASARLLIVGLAPGLKGANRTGRPFTGDYAGDLLYATLLSHGFASGSYGARPDDG
                     LSLVDCRITNAVRCVPPENKPTPAEIGACRPFLVATIASMPRLIAILALGRIAHDQVL
                     TTLEARRAAYPFAHGRRHDLGRYNLYDSFHCSRYNTNTGRLSAEMFMDVFRQIRIDLD
                     TRQAAAT"
     gene            7541..8788
                     /locus_tag="PK264_16325"
     CDS             7541..8788
                     /locus_tag="PK264_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HRD77474.1"
                     /translation="MDGKTRVLIADDDAIYREMAAASLPESMHATTVADGAEALAALR
                     VCRYDIVIVDLEMPEIDGLGVMKAIRSSGLNVDTPVIVITGHDDANAIQVAYDAGATS
                     FLAKPLNWVLFGPHVEFVLRSARTELELRDAINATEFLGELKSRLMNALGHEFREPLR
                     TLLSLTELMRQEVYGPLGSPQYRGFVTDMGRAIGQINSSLMKLLWSGRALMEQLSVRS
                     EPIDLGQLISEVVLPLQSAADRRGVLVSIKGSRKYVVQGDKALLGQALRGMLESTLKL
                     APRGSEVSIDISIAEDGGLCLAMTDVGSAPSEQIIASVNGDALTARPFTPQEHRDVCL
                     EVAKVLFDAHQGRLNVRASADGNVSTLTLPKARILPVGEATGSDVASPGAPSVSDTGA
                     DVFGTRRKDSEPAVFARSGHAAR"
     gene            complement(8795..>9449)
                     /locus_tag="PK264_16330"
     CDS             complement(8795..>9449)
                     /locus_tag="PK264_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRD77475.1"
                     /translation="LAFLATDAALPPVLLQELLSEAVARSFNAITIDGDTSTSDTVLL
                     FATGAAAKRGAPRIADAADPALAEFDAALHSAMRELAIMVAKDGEGLSKFVTFEVLGA
                     ENDRAARRIAMSCANSPILKTAIAGEDPNWGRVVMAVGKSGEAADRDRLAIWFGPHLV
                     AREGERAESYVEKTVAAYMKNAEIVIRVDVGIGGGRATVWTCDLTHDYVSINADYRS"
BASE COUNT         1581 a         3191 c         3001 g         1676 t
ORIGIN      
        1 cgttgatggt cgagacgacg gcggtcttct tgccggccga gccgaacttc ttgatctccg
       61 cgacgatcgt ctgccagtcg gaatggccga acggcgtgta gttgatcatg atgtcagcgg
      121 gcgccacgcc cttggctttc aggtaggcct cgaggatctt gttcgtggtg cgcgggtaga
      181 cgtagtcggt accggcgagc acccaccgct tcacgtcgcc cccatccttg ctcatcaggt
      241 agtcgacagc cgggatggct tgctggttcg gcgcagcacc cgtgtagaag acattgcgct
      301 cgctctcctc gccctcgtac tggacggggt agaagaggat gctgttcagc tccttgaaca
      361 cggggagcac ggacttgcgc gagacgctcg tccagcagcc gaacaccgag gcgaccttct
      421 tctgcgagat gagctcgcgc gccttctcgg cgaacagcgg ccagttcgag gccggatcga
      481 cgacgacggc ctcgagcttc cggccgagga ggccgccctt cttgttctgc tcctcgatca
      541 gcatcagcat gacgtctcga agggtcgtct cgctgatcgc catggtgccc gacagcgaat
      601 ggagaacgcc gaccttgatt gtctcgccct gggcgaacag atgccgtccc ccgactgcgg
      661 ttgccgcgac cgtcgaaagt cccccgagaa gggtgcgcct cgtcaacgac atgagaatct
      721 ccgtgcttgt cgttaagact ggttaacgat cagcaaggcc tgtgccagca acgttgcggc
      781 gcacaaaatt cgatgctttg cctttgattc tacaagataa ttttcgatca ccgtggatcg
      841 gatgcccgcc accggggaat gcaaactgcc ttgtttggag gcagctgctg cggaaaaatc
      901 attcgatacg cgactgctga cgttttcttg ggcggcagct ggaaaaacgt gcagttcggg
      961 tccagtctcg acttttggat acacgctcaa tcggagtgat cgtcatgttt gaagctcgtc
     1021 cggaagcttc ccgttcgcat cgaggacttc ttcaggcgtg cggaccgctt ggcattttgg
     1081 tgacgctggt catgctcctc atagtcgtca cgacggcgat gcccaggcct gtgacccgcc
     1141 ggatacctat gcggtgacta tcgagaattt cgacgcaagg gtcgtaagcg caatcgagcg
     1201 gttcctggcg tcgtttcgcc gtggtgaagc agcaatgcgt gttcgggccg atagccgcgt
     1261 cgtcgagctc gagtatcggt tcggtgccgg cagcgagcgt ctatacagga atttccggtt
     1321 gatgctcggg cagctgggcg acgccggtac tgttgaattc gggcccggca acaaattcta
     1381 cctggtcgct gcagacaagg gagcagccgt gccgccgccg ccctcgggcg ggccgtcacc
     1441 agcgccatct ggtgccaaga ttgcaaccaa gcgcctgctc gtcgtcgtcg acgacgctga
     1501 tcggacttcc actcacgctt cgaacaggag catgcgcatg ctcctcgacg cggtctccac
     1561 tggcctgacg gagcggagct ttgaggtcat ctcgccgatg ggcgtggacg tcggcacccg
     1621 cgtgaagcag ccgaccaagg ctgcgctcga ggtaacggcc gcgattccgg gtggcgcgcg
     1681 catcctgctg gtcaagctca cgggcgatgg caagcggttg gggcccggag gcgcagcatc
     1741 gcctctcgtt gctcgcgccg tgctccgcct cgccgacgca accgatgcag tgctcgtgtg
     1801 ggaaggcagc gccgatgcgc agctggagca ctcctgtgcg cgtgacgcca actgtctcgt
     1861 cgatgcccat ggcagactgg cgaggtcgat cctcgcatcg ggctgggcac atatcgcggc
     1921 caagctcaag tgactatcgc gccagttcct tcaacccgcg ccggaggagt ttggccgtat
     1981 cagcctcctc gccgaggtcg cgttgggcgg cggcgactgc ctgcgcggcc tgggccggct
     2041 gatagccgag attgacgagg cccgagatcg cctcggcgac cgcatgggac gcgaccggca
     2101 taggcgtcgg catcgtggcg ccagcagcag tgggaccgat accgagcatg gaaaggccag
     2161 gtgccttgtc gcgcaattcc gccgtgatgc gctgcgcgag cttcttgccg acgccgttcg
     2221 cctctgcgac cgccccccag ttctgcagcg cgatggcgct ggcgaggtct tgcggcgaga
     2281 gcacgctcag caccgaaagc gcgaccttgg cgccgacgcc ctgcacgctc tgcaaggtgc
     2341 ggaaccagct ccgctcgacc tcgctcgtga agccgaacag gcggatcgcg tcctctcgca
     2401 catgcgtctc gattgagagc gtcgcgtcgt ccccaggttt cagggagcgc aaggtccggg
     2461 ccgagcagtt gacctcgtag ccgacgccgt tgacatcgat gaccgcgtgg gcctcgccaa
     2521 cggcgtcgac cttgcctctg agcttgccga tcatggggcc ctctgccatg tgtcgcgcgg
     2581 cctgccacac cgcgcgaccg gtctagcaaa ggtctggcgc catgtcgctc gcagagagac
     2641 gtcctcagtc tctggcgcgc gagttatgcc atcagcatgt agtcgctggc ccaggcgtca
     2701 gcatcgccgc cgcgcacacc cgagagcatg acgatatcga cgaactgtcc atccacgttg
     2761 gccgagacca tggtaccctc gacggtcgag gtcatgcgga tgctgttctc gagcgtcggc
     2821 acgtaggact tcacaaaatc acggagatcg agcgtgtcac ccgtgttgaa gtcagagatc
     2881 acgtccacgc cctgatgcac gccgccgacg agtacgtctt tcatgaggaa agtgtaggtg
     2941 tcgttgccgg cgccgcctgt gtagctatcg acgcccgccg cgccacggaa gaggtcattt
     3001 cccgagccgc cgacaaatat gttgccactc tggtcgccgc gcagcatgtc gtcgcccgac
     3061 gagccgacca cgcgctcgat gccccagacc gcatcggccc ccgtcagatc gccgaaagcc
     3121 gtgtgcgcgt tgaggtcgac gaccacggcg ccggcgcccg agagatcgag cgtgtcgaag
     3181 cccgagccgc cgtcaagata gtcgtcgccg ccgccggcaa cgagagtgtc gttgcccgag
     3241 ccgccatcga ccatgtccat gccggcatca tccatgagaa gatcgttgcc ggtgctgccg
     3301 cgcagcgcat catcgccgtt gccaccttcg aggcggtcgt cgccgctatc tccggcgaga
     3361 gagtcgttgc catcgccacc gcgcagcgtg tcgttctcgg tgccgccggt cagagtatca
     3421 tcgtcgccaa aaccgtcgag cacgtcattg ccggcgccgc cgaagatgac atcgttcccg
     3481 gcctcgcccc agatcgaatc atttcccgtg tcgccgtaga gcgtatcgct tccactgccg
     3541 ccgcgaaggc tgtcgtcgtc cgcgccgccg cgcaggaggt cattgccttc tccgccatcg
     3601 agcacatcgc ggccgagccc tccgtagatc gcatcgttgc ctgcatcacc gaagagccgg
     3661 tcgtcgccgt cctgaccgta gacggcatcg tcgccgccat ccgacgtgac gacgtcgttt
     3721 ccagcgtcgg ccataatctg atcctgcagg gtcgacccga agatccgatc atcgccaacc
     3781 gtgcccttca tcgacgccat tcccagcctc ccgcgcgtgc agccgtgctc cgcttttgcg
     3841 aaaaagcggc gccaaagccc cgagtatttc cgactatttc agttccaatc gcagctcccg
     3901 ttttgagacc ttagcaacac ctaattccaa ttccgttcaa aaggccgagt tagtttttcg
     3961 caagattgct tcaaatttga tgcgctttgc gcggatgctg gctggcgccg aggcggacga
     4021 gggccggccg atgcgcgtcg ccgcatgact gctcatcaag ggccgccagc gaacaacagt
     4081 tgccgtgcag accaggaggc gccagagtgc gccgcgatcc cgatcgccac cacggagacg
     4141 acccatgcct gctcccgacg ccccggttct ctatgagcag gatggcggca tcgtcactgt
     4201 gacgctcaac gcgccgaaga cccgcaacgc cctcgacgat cccgtcatcg acggcatcgt
     4261 cgaggcgtgc cggcgcatca acgccgacat gagcgtgcgc tgcgccatca ttacagcgaa
     4321 gggcgaggcg ttctcctcgg gcggcaacgt caaggcgatg cgcaacggag agggtatgtt
     4381 cggcggaact cccgtcgata tgcgccgcgg ctaccagacg acgatccacg gcattccgcg
     4441 ctcgatctac gccctcgagg tgccgacgat ctgcgccgtc aacggcccgg cgatcggcgc
     4501 cggctgcgac ctcacattga tgtgcgacat ccgcattgcc ggtcggaagg cccagttcgc
     4561 cgagagcttc ctgcgcgtgg gcctcgtctc gggtgacggc ggcgcctggt tcctgccgcg
     4621 cgtcgtcggc ctctcgcgcg cctacgagat gacgctcacc ggcgacgtcg tcgatgccga
     4681 gcgcgccgag cggatcggcc tcgtctcgaa ggtcgtcgat gacgacatgc tgatggacga
     4741 ggcgcgggcg ctcgccgccc gcattgccgc gcacccgccg cactccatcc gcctcaacaa
     4801 gcgactgctg cgcgagagcc agggcgtgag cctggacgta gcgctcgagc tcgcctcggc
     4861 catgcaggcg ctggtgcaga gcacgagcga ccagaaggaa gcggtggcgg cgctgatcga
     4921 gaagcgcaag ccgagctaca agggcaagtg atcatggcgg gggcactcga gggagttagg
     4981 atcatcgaca tgacggccgt ggtgctaggg ccggttgcga cgctgcacct cgccgacatg
     5041 ggcgccgacg tcgtcaagat cgagccgccg gagggcgacg tgatgcgcaa cgcgggtcac
     5101 agccccgtgc gcaagatggg gccgatcttc ctcaacgcga accgcaacaa gcgctcgctg
     5161 gccctcgacc tgaaaaagcc cgcggcggtc gacgtcgtga agcggctcgt cgccaaggcc
     5221 gacgtgttcg tgcacaacaa ccggccggcg gccgtcgagc ggctcggcct cggctatgac
     5281 gacctcaagg tcgtcaatcc gcgcctgatc tacgctttcg cccacggcta caagcgtacc
     5341 ggcccctatg gccacatgcc ggcttacgac gacctcgtcc aggcagcgag cggcgccgcc
     5401 atgctgcagt cgcgcatcga cggcggcgag ccgcgcttcc tgccgacgct cgttgccgac
     5461 aagacgacgg gtctgcatct cggcatggcg atcctggcgg cactctatgc gcgcgagcgc
     5521 acaggcatcg cccagcagat cgaggtgccg atgctcgaat cggttaccgc cttctggatg
     5581 ctcgagcacc tcttcgatca taccttcgtg cctccgcgcg gcgaaatggg ctacgcgcgc
     5641 atcctgaacg agttccgccg cccctatcgc acgcgcgacg gtttcgtcat ggcgctgccc
     5701 tatacggcca agcactggag cgccttcacg gacggaaccg ggcgcaagga cctcgccatc
     5761 gacccgcgtt tcgtgacgct cgaggcgcgc gccaacaatc aggcggcggc catggccgcc
     5821 attgccgaga tcatgctgac gcgcacgacc ggggactggc tcgcgttctg cgagaagaac
     5881 gacatcccgg cacagcgaat caacgatctc gaggatctgc cgaccgaccc gcatctcgcc
     5941 gcgacgagct tcttcacgcg gcgcgagcat ccgaccgttg gtcccgtgct gacgatggcg
     6001 agcccgttca atttctcgga gacgccgacg agcttccgcc gccacgcgcc gacgctcggc
     6061 ggcgacggcg ctgaggttct cacagaggcg ggcttctcgg agtccgagat cggcgcactt
     6121 cggtcggaag gcgcgctcgt cgaggatgcc ggtcggccgc gcgacgcggc gccgagttct
     6181 tgacgccgcc gccctcgccg cgtaacgcca ccccgatcgc cacgcacaag tggcaagtgg
     6241 gggcgaaatg accacgcgca gcatgtcacc cgaggcccag agccggcccg tcaatgcagg
     6301 tgatcttgcg accatttccg ggttgcatgc gcgcgtcttc ggtcctggcc ggttcgttcg
     6361 cacggcctac cgcgtccgcg acgggctgcc gctcgtctcg cctttctgcc gcgtcatcgt
     6421 gctcggaggt gagatcatcg cggcgctgcg cttcgcgccg gtgacggtcg gcggcgtagg
     6481 ccgggcgctg atgctcggcc ccgcagcggt gacgcctgag cacgccaacc tcggctatgc
     6541 gcgccggctc atcgcggaag ggcacgccgc ggcccgggac gcgggcatcc ggctcgtcct
     6601 gctcgtcggc gacgagccct actacggccg tctcggtttc gtgcgcgtcc cgaaggggca
     6661 gatgagcatg ccgggaccgg tcaatccgga ccgcctgctc gccctggaac tcgaggccgg
     6721 cgcgctcgcg agctatcggg gccttatcgt cggcgacgcg gctgccacgc catgagcccc
     6781 tcagtggtgg caccatccgc gagctgcgac cccggccgcg actgcccggg ctgtgagcgc
     6841 ctcgtcgcgt tccgcacgaa gcaccgcgcg acggagccga cttggcacaa cgcgccggtc
     6901 gcgagtttcg gcccggcgag cgcgcgcctg ctgatcgtgg gcctggcgcc gggcctcaag
     6961 ggcgccaacc gcaccgggcg gccgttcacc ggcgactatg ccggtgatct cctctacgcg
     7021 acgctccttt cacacggctt cgcatccggg agctatggtg cccgccccga cgacggtctc
     7081 tccctcgtcg actgccgcat caccaatgcg gttcgctgcg tgccgcccga gaacaagccg
     7141 acaccggccg agatcggtgc ctgtcgcccg tttctcgtcg ccacgatcgc gtccatgccg
     7201 cggctcattg ctattctggc gctcggccgc atcgcgcacg accaggtatt gacgaccctc
     7261 gaggcccgcc gcgcggccta cccgttcgcg catggccgtc gccacgacct cggacgatac
     7321 aacctttacg acagctttca ttgttcaagg tacaacacca acaccggccg gctctcggct
     7381 gaaatgttca tggacgtatt caggcagatt cggatcgatc tcgacacgcg gcaggcggct
     7441 gccacgtaga gtaaacggtt gttcaatgtt caagcgctag gttccgacga aatgcgcaga
     7501 gttgggaaga agcggcgcaa tcgttcatgg gagccagcgc gtggacggaa aaacccgggt
     7561 tctgatcgcg gatgacgacg ccatctatcg cgagatggcc gcggcttcgc ttcctgagag
     7621 catgcacgcc accacggtcg ccgatggcgc cgaggcgctc gccgccttgc gcgtctgccg
     7681 ctacgacatc gtgatcgtcg acctcgagat gcccgagatt gatggcctcg gtgtgatgaa
     7741 ggccatccgc tcgagcgggc tcaacgtcga cacgcctgtt atcgtcatca ccgggcacga
     7801 cgacgccaac gccattcagg tcgcctacga cgccggcgcc acatcgtttc tggcaaagcc
     7861 cctcaactgg gtgctgttcg gaccgcatgt cgagttcgtg ctccgctcgg ctcggaccga
     7921 gctggagctt cgtgacgcaa tcaatgcgac ggagttcctc ggcgagttga aaagccgatt
     7981 gatgaatgct ctcggccatg agtttcggga gccgctccgc acgctgttga gcctcaccga
     8041 gctgatgcgc caggaggtct acggccccct cggctcgccg cagtaccgcg gcttcgtgac
     8101 ggacatgggc cgtgcgatcg gtcagatcaa cagctcgctc atgaaactcc tctggtcggg
     8161 tcgcgcgctg atggagcagc tctccgtccg ctcggagccg atcgacctcg gccagctcat
     8221 cagcgaggtc gttctgccgc tgcagtccgc cgctgaccgg cgcggcgtcc tcgtctcgat
     8281 caagggttcg cgcaagtacg tcgttcaggg tgacaaggcg ctccttggtc aggcactccg
     8341 cggcatgctc gagagtacgc tgaagctcgc gccgcgcggc agcgaggtgt cgatcgacat
     8401 ctcgatcgcg gaggatggcg gcctctgcct cgccatgacc gacgttggtt cggcgccttc
     8461 ggagcagatc atcgcgagcg tcaacggcga cgcattgacc gcgcggccgt tcacgccgca
     8521 agagcaccgc gacgtctgcc tggaggtggc gaaagtcctc tttgacgccc atcagggccg
     8581 gctcaacgtc agagcctctg ccgatggcaa cgtgtccacg ctgacgcttc cgaaggcacg
     8641 catcctgcca gtcggcgagg caacgggctc tgatgtcgcg tcgcccggtg ctccctctgt
     8701 gagcgacaca ggtgccgatg tcttcggcac gcgccgcaag gacagcgagc cggccgtgtt
     8761 tgcccgatcc gggcatgcgg ctcgctgacc tctttcaact acggtagtca gcgttgatcg
     8821 acacgtagtc gtgagtgaga tcgcatgtcc agacggtcgc acggccgccg ccgatgccga
     8881 catcgacgcg aatgacgatc tcggcattct tcatgtaggc cgcgaccgtc ttctcgacgt
     8941 agctctcggc gcgctcgcct tcacgcgcaa cgaggtgcgg gccaaaccag atggcgagcc
     9001 ggtcgcgatc ggctgcctcg cccgacttgc cgacggccat cacgacgcgc ccccagttcg
     9061 gatcctcgcc ggcgatcgcc gtcttcagga tcggcgaatt ggcgcagctc atggcgatgc
     9121 ggcgcgcggc gcggtcgttc tcggctccga gcacctcgaa ggtgacgaat ttcgagagcc
     9181 cttcgccgtc ctttgccacc ataatggcga gttcgcgcat cgcgctatgc agcgccgcgt
     9241 cgaactcggc aagtgccggg tcagccgcat ccgcgatgcg cggcgcgccg cgcttcgccg
     9301 ctgcccccgt cgcgaacagg agtaccgtat cgctggtcga ggtatcgccg tcgatcgtga
     9361 ttgcgttgaa cgagcgcgcg accgcctcgc tcaggagctc ttgcagcagg accggcggca
     9421 atgcggcatc ggtggcgaga aaggccagc
//
LOCUS       DAORNH010000174         9408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5403_length_9408_cov_2.575858, whole genome shotgun sequence.
ACCESSION   DAORNH010000174 DAORNH010000000
VERSION     DAORNH010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5403_length_9408_cov_2.575858"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>323
                     /locus_tag="PK264_16335"
     CDS             <1..>323
                     /locus_tag="PK264_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77476.1"
                     /translation="PSEIVVGTWSGAYGKAQQLAVMTPYTETTGIRVRVDTYARSGRD
                     ADRLKRDGAAADVLDMSSGALEAACAEGLLEAPDTGALNASSRGGPITEDFIAGGIAP
                     CGIAS"
     assembly_gap    324..486
                     /estimated_length=163
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <487..1086
                     /locus_tag="PK264_16340"
     CDS             <487..1086
                     /locus_tag="PK264_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD77477.1"
                     /translation="PADIYKVLSTPEGLKRAIDEVRVLAPHAVWWDNAAGAIAHLRSG
                     QAAIALAYSGRAFQEFATTPAIATLWDGQVYDLNYWAVPKSARHKDAAWEFVAFATAP
                     ERLAATARHFPYGPARRSAVGLVGRHAVLGIELASHLPTAPANMTRALRFDAAWWAEN
                     EARVTDLFNAMINETGAASAAVPGKAAPKTTAPPPKRVR"
     gene            1201..1569
                     /locus_tag="PK264_16345"
     CDS             1201..1569
                     /locus_tag="PK264_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HRD77478.1"
                     /translation="MPRSTRETSERTTTLRRFAILMMAGAALLWPAAALSQSAGDPKR
                     GLDFAERTCSDCHAVGLLGVVSPRPDAAPFVVIARVPGINERSLLVFFQTPHKNMPNL
                     IVTGQDRDDLIAYIMTLKKD"
     gene            complement(1573..2823)
                     /locus_tag="PK264_16350"
     CDS             complement(1573..2823)
                     /locus_tag="PK264_16350"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019478 - D-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HRD77479.1"
                     /translation="MRVVVLGAGVIGVTTAYELASDGHDVTVIEKLSEPASDTSYANA
                     GLVAPGHAYTWASPKAPRILLRSLMKEGQALQFRPSLDPKLWSWSLQFLRNCTATAAR
                     INTKRKVRLCMYSQEQLDRVIRETGVDHHRVTGGLLYLYRTPQSFERGTANTRILTDE
                     GLTLKPVSADEAARIDPALEPVKGDFAGAIYCPSDESGDARAFTQSLARHLAERMGVK
                     FLFDAEITGFDVAGDRVRAAITTGGRVDGDEFLFCLGVMSPFLARTLGAKLPIYPVKG
                     YSVTMPIGVRNGAPRLGGVDEDNLVAYARFGDRLRVTATADFAGYDRTHTPADFTHML
                     GVAKRIFPNGADFEKPSYWAGLRPMTPEGTPIIGRGRYRNVVFNTGHGHIGWTMSCGS
                     ARIAADLVAGRRPAIPLDGMHVKG"
     gene            2975..3688
                     /locus_tag="PK264_16355"
     CDS             2975..3688
                     /locus_tag="PK264_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77480.1"
                     /translation="MSIVRSGFLALLMLLAMSVPCLGQLPSGWSQSRRGDALVFQSPA
                     DRGGNIVLVLVPAAQSSSDDFEDWFRERLRSGGENIGSIVERQSFERDGDWLKSSMRV
                     ERGNGDELVVAGFAYEASRGYQLMLVVVPAGIEESDRRVREAVDLVIALRRTDDGGST
                     AAPAPVPGGPGSSSTRPPPPPPPKTSDPTRPGQRCRMVSRWVNDSKMGTSCTGFGANR
                     VCVPTFTPGMKQVWERVCD"
     gene            complement(3706..4826)
                     /locus_tag="PK264_16360"
                     /pseudo
     CDS             complement(3706..4826)
                     /locus_tag="PK264_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
     gene            complement(4911..6248)
                     /locus_tag="PK264_16365"
     CDS             complement(4911..6248)
                     /locus_tag="PK264_16365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRD77481.1"
                     /translation="MTAAPALPLPSPVAAIPSAVSADLLARPERAWTCLMGALVALVG
                     TFIGWAAIATVQEVTTGRGRIIPASKIQVVQSLEAGIVREILVREGALVREGDVILRI
                     DPTIAGSSLGEVREKMLGLRAIIARLEAEVEDRPVVFPAELAEQRPDIVTSQRDQYEA
                     RRRELVGSLGALDGQVRQRTQEIAEAEARLVTLRRALEIAGEELEMIRPLEKTRAASR
                     SELLAAEAKVNDIRGSLKATELAIPRLQAALEEARGRRAERIEAARGEALQKLTSARV
                     DLAALAEASRGNEDRLGRTTVKAPASGIIKTVNVTTVGQVIQPGHSVIEIVPMDSQLL
                     VEVQVRPQDIAFLRPGQAALVRLTAYDFAIYGGLKAKVEQIGADSVTTEKGESFYVVR
                     VRAETAHLRHSGEVLPLIPGMVADVDVITGSKTVLAYLVNPLTRMRHTALRER"
     gene            complement(6245..>8457)
                     /locus_tag="PK264_16370"
     CDS             complement(6245..>8457)
                     /locus_tag="PK264_16370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HRD77482.1"
                     /translation="AMALSSKAGEAEGDRLAQAIVDLAAHYGEATTVTVITRGLPLVG
                     GRLSVEHAAEAAVRASLTLEAASIGLDELRDLDLPAIVLINDGSADILWSIRRAGDGR
                     PVDVEVSEPGAPGTRSHIAYAEYAAAHDGRVLRVRPPSVTRAAADGLPPAPQPGWLLQ
                     AFKSSRRLYVQAIIATLAVNLLALAMPLFTMNVYDRVLPNAAVETLWALSIGVILATL
                     FDFAIKTLRSRVIDGVGRRADVLLANRIFGRLLGARLGVRGSTGVRANTLREYETVRE
                     FFSSATLTAFGDLPFLVLFLAMISVVAGSLVWVPLLSIPIVLAIAWVTQRRIMRLSET
                     AFRASAQKSAVAVETIGGLETLKAAGAESFAAGRWERAVADGVRNGHALRDLSSIGMG
                     LVGAVQTLAQVVMIIAGFYMVQSGSLTMGGLIAATMLAGRALQPLGQAAALITRLHQA
                     KLAYRMLDEIMTAPQERTDDARLILPSAVRGSIAFEAVTVAYEKDAPPALRDVSFSIA
                     AGEAVAIVGGIGSGKTTALKLIDALLQPTSGRVLVDGLPAAQIEPAILRASVGLALQD
                     GEVFQGTLRENIMLGRTGWSDSQILAALAAASAADWVMRLPKGLETRVGERGQGLSSG
                     QKQSLTLARAIVGKPTVVLLDEPTSAMDPTSERETVKRLKAALAGRTVVLVTHRPALL
                     DLVERIIVLDGGRKVLDGPKASVLASMRAMSERAASERAKAPVRVGALQPGGAT"
     assembly_gap    8458..8467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8557..>9408)
                     /locus_tag="PK264_16375"
     CDS             complement(8557..>9408)
                     /locus_tag="PK264_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRD77483.1"
                     /translation="ANAAAVALEAEGRLREAETLFRSVTGRAPGKLASVSFPANHLPR
                     SIDAAVAEAIAAAPSVIATAHDATAAHAAVGVAHSRHYPRLNLELSSNHGWNVAEDHD
                     RSIDARAMLVVRWNLLNGGIDKAREWEARARALEASEISENTKRIVERETRASWNAVD
                     IARSRIPLLVKQVHQTRLTRAAYGSQFDAGQRRLLDLLNIQAEVFLAEAALRTEELVR
                     LYNSYRLLAAMGRLVPALGLEPPREAVMPHAPTVIDGWRDGFANWRPTVTYHVSPVPS
                     APAKPVK"
BASE COUNT         1452 a         3192 c         3108 g         1483 t
ORIGIN      
        1 agccgagcga gatcgtcgtc ggcacctggt ccggcgccta cgggaaagcg cagcagctcg
       61 ccgtcatgac gccctacacc gagacgaccg gcatccgcgt gcgggtcgac acctacgccc
      121 gctccggccg cgacgccgac cggttgaagc gtgacggcgc cgcagcggac gtgctcgata
      181 tgagctccgg cgcgctcgag gccgcctgcg ccgagggtct cctcgaggca ccggacacag
      241 gcgcgctcaa cgcatcgagc cgtggcgggc cgatcaccga ggacttcatc gccgggggga
      301 tagcgccgtg cggcatcgcg agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnncccg ccgacatcta caaggtgctc tcgacgcctg aaggcctgaa gcgggccatc
      541 gacgaggtcc gcgttctcgc gccgcatgcc gtctggtggg acaatgccgc tggcgccatc
      601 gcgcacctgc gcagcgggca ggcggcgatc gcgctcgcct actccggccg cgccttccag
      661 gagttcgcga ccacgcccgc gatcgcgaca ctctgggacg gtcaggtcta cgatctcaac
      721 tactgggccg tgccgaagag cgcgcggcac aaggatgccg cgtgggagtt cgtcgccttc
      781 gcaaccgcac ccgagcggct cgcagcaact gcacggcact tcccatatgg tccggcgcgg
      841 cgctccgccg tcggcctcgt cggacgtcat gccgtgctcg gcatcgagct cgcctcgcac
      901 ctgccgacgg cgccggccaa catgacgcgt gcgctccgat ttgatgccgc ctggtgggcc
      961 gagaacgagg cgcgcgtcac cgatctcttc aatgcgatga tcaacgagac gggggcggcc
     1021 tccgcggccg tgccgggcaa agccgcaccg aagacgacgg cgccgccacc gaagcgggtc
     1081 cgctgatttc cgttttggac ggcttgacct atctcaacgc gcagccgtgg caggggccta
     1141 ggctcgtcat tgaagcgcga cggcagttcc gccgctgcgt gatcgcggac gagatcgccc
     1201 atgccccgca gcacgcgaga gacatccgag cgaacgacca cccttcgtcg cttcgcgatc
     1261 ctgatgatgg caggcgcggc cctcctctgg ccggccgccg ctctctccca atctgcaggt
     1321 gatcctaagc gcggcctcga ttttgccgag cgcacctgct ccgactgtca cgccgtcggc
     1381 ctcctcggcg tcgtttcacc acgacccgat gccgcaccgt tcgtggtcat cgcgcgcgtg
     1441 cccggaatca acgagcgctc gctcctggtc ttcttccaga caccgcacaa gaacatgccg
     1501 aacctgatcg tcaccggcca ggatcgcgat gacctcatcg cttacatcat gacgctgaag
     1561 aaggactgac ggctacccct tcacgtgcat gccatcgagc gggatcgcgg gccgccgtcc
     1621 agcgacgaga tcggcggcta tgcgcgccga gccgcaggac atcgtccagc cgatgtgacc
     1681 gtggccagtg ttgaagacga cgttgcgata cctgccgcgc ccgatgatcg gcgttccttc
     1741 gggcgtcatg ggcctgagcc ccgcccagta ggagggcttc tcgaagtccg ccccgttcgg
     1801 gaagatgcgc ttggcgacac ccagcatgtg cgtgaagtcg gcgggcgtgt gcgtcctgtc
     1861 gtagccggca aaatcggccg ttgccgtgac gcgcaggcga tctccgaagc gggcgtaggc
     1921 gacgaggttg tcttcgtcca cgccaccgag gcgcggggcg ccgttgcgca ctccgatcgg
     1981 catcgtcacc gagtagccct tcaccggata gatcggcagt ttcgcgccga gcgtgcgcgc
     2041 gagaaacggg ctcatcacgc cgaggcagaa caggaactcg tcgccatcga cacggccgcc
     2101 tgtcgtgatc gccgcgcgca cgcggtcgcc cgccacgtcg aagcccgtga tctccgcatc
     2161 gaacaggaac ttcacgccca tgcgctcggc gagatggcgg gcgagcgact gcgtgaaggc
     2221 tcgcgcgtcg ccgctctcgt cggaggggca gtagatcgcg ccggcgaagt cgcctttgac
     2281 cggctcgagc gcgggatcga tgcgcgcggc ctcgtccgcc gagaccggct tcagcgtgag
     2341 accctcgtcc gtcaggatgc gcgtgttcgc cgtgccgcgc tcgaagctct gcggggtgcg
     2401 atatagatag aggaggccgc ctgtgacgcg gtgatgatcg acaccggtct cgcggatcac
     2461 gcgatcgagc tgctcctgcg agtacatgca gagccgcacc ttgcgcttcg tgttgatgcg
     2521 cgccgccgtc gccgtgcagt tgcgcaggaa ctgcagcgac cagctccaca gctttggatc
     2581 gaggctcggg cggaactgca gcgcctggcc ctccttcatg agggagcgca gtaggatgcg
     2641 cggggctttc ggagaggccc acgtataggc gtgccccggt gccacaaggc ccgcgttggc
     2701 atagctcgtg tcggaagccg gttcggagag tttttcgatc accgtcacgt cgtgaccgtc
     2761 tgacgcgagc tcatacgctg tcgtcacgcc gatcacgccg gcaccgagca ccacgactct
     2821 catgtccacg cctccgccaa tcctgccgct cgccctcagt catgtcggca aatgcggatt
     2881 gctgcaacct cgggcgcgag gcatagtgat cgtgcctgcc gcccatgatt ggcgtctgag
     2941 cttccgacgc cgagcaccat tctggatggc gtccatgtcg atcgttcgat ctggttttct
     3001 tgcactcctt atgctactcg cgatgagcgt tccctgcctc ggccagctgc cgtccggctg
     3061 gagccagtcc cgtcggggcg atgcactcgt gttccagtct cccgcggatc gaggcggcaa
     3121 cattgtcctt gtgctggtac ccgcggccca aagctcctcg gacgatttcg aggactggtt
     3181 ccgcgagcgt ctcagatccg ggggcgagaa catcggctcg atcgtcgagc ggcagagctt
     3241 cgagcgcgac ggcgactggt tgaagtccag catgcgggta gaacgcggca atggcgacga
     3301 actcgtcgtc gccggcttcg cctatgaggc ctcgcgcggc tatcaattga tgctggtggt
     3361 cgtgccggca ggcattgagg agagcgaccg ccgcgtccgc gaggccgtcg atctcgtcat
     3421 cgcactccgg cgcaccgacg acggcggcag cacggcggcg ccggcacctg ttccgggcgg
     3481 gccgggcagc agcagtacgc ggccgccgcc gccacctcct ccgaaaacct ctgatccgac
     3541 gcgtcccggc cagcgctgcc gcatggtctc ccgctgggtg aacgacagca agatgggcac
     3601 ctcgtgcacc ggcttcgggg ccaaccgcgt ctgtgtgccg accttcacgc ccgggatgaa
     3661 gcaggtctgg gagcgcgtct gcgactgagc ttgcggcccg ggtcgtcagc cgggcgcccg
     3721 gccgcggagc cgcgttgcca ggatgtcagc gaccgccttg ctcgcctcct tgaggccctg
     3781 gtcaccgccg aactccatcg gccggagccg gcgcgaggca aacccctcct ccaccgccgc
     3841 ctcgagatgg cctgctgcct ccgagagttc cgggatggcg tgcttttcgc cgagccagtc
     3901 gagcatcatc gccgccgaca ggaacgtcgc cgtggggttg gccttgtcct gccccgtgat
     3961 gtcgccgagc cgtgcgccgg ctggaagagg ccgtgcttgt cgccgatctc cgcgcacggt
     4021 gccatgccca tgccgccgac gagcccgccg ccgagatccg agatgatgtc gccgaacatg
     4081 ttctccatta caaggacgtc gaagtcccag ggcctgcgca cgaggtcgag cgcctgcgca
     4141 tcgacatagt tgtagcccgt gcggatgtcg gcgaaacctt tggcacgctc gtcgaagatc
     4201 ttgcggaaga acgcgaaggc gcgaaacacg ttcgacttgt cgacgcaggt caccaggcct
     4261 ttcgcgggat tgcgcgcctt gcgccgccgg gcgatgcgga acgcggtatc gcagagcttc
     4321 tcggtcgtcg cccgcgtgat gcgcagcgtc tcgcgcgcct cgctgtcacc gatgatctcg
     4381 ccgcgcccct gcgtgtagaa gaggccctcg gtcgactcgc gcaggatcac gaggtcgatc
     4441 atctgcgcac gctcgtcgag cagccggcgc ggcgtgttcg ggaatgcctt gacgggccgc
     4501 acaccagcgt agagcccgaa ctcctcgcgc atgcgcaggt gcggggcgac ctcagtgccc
     4561 gacggcagcc gcacgctcgg cagacccatg gcgccgagta ggatggcgtc cgccgcgcgc
     4621 gccgcctcaa aggcctcgac cgagatgtcc ttgccgctct ccttgtagta ggcggccccg
     4681 gcgtccgggc gatcctcgaa ggccagcgtg aagcgcccga tcgccttctc gacgagcccg
     4741 agcaggtgga tcgtcggcgg catcagctcg cggccgatgc catcgcccgg cagcaccgcg
     4801 atgttgagct tggtattgga gggcatgcgc atctctctcg ttgagctcgg atcgaggcga
     4861 gcttagccgc aactcgatcg ctgcgcactc gctcgtgacg cgaggagcgc tcagcgctcg
     4921 cgcagtgccg tatgccgcat gcgcgtcaac ggattcacga gataggcaag gaccgtcttc
     4981 gatcctgtga tgacatcgac gtcggcaacc atgcccggaa tgagcggcag cacctcgccg
     5041 ctgtgacgca agtgtgcggt ctccgcgcgc acccgcacga cgtagaagct ctcgcccttc
     5101 tccgtggtga cgctgtcggc accgatctgc tcgaccttgg ccttgaggcc gccgtagatg
     5161 gcgaagtcgt aggctgtgag gcggacgagt gcagcctgac cgggccgcag gaaggcgata
     5221 tcctgagggc gcacctgcac ctcgacgagc aactggctat ccatcggcac gatctcgatc
     5281 acgctgtggc cgggctggat cacctgcccg acagtcgtaa cattgacggt cttgatgatg
     5341 ccgctcgccg gcgccttgac cgtcgtgcgc ccgaggcgat cctcgttgcc ccggctcgct
     5401 tcggccaagg ccgcgagatc gacgcgggcg ctcgtcagct tctgcagtgc ctcacccctg
     5461 gccgcctcga tccgctcggc acgccgtcca cgggcctcct cgagcgctgc ctgcagacgc
     5521 gggatggcga gttcggtcgc cttcagggag ccgcggatgt cgttgacctt cgcttcggcc
     5581 gcgagcagct ccgagcgtga ggcggcccgc gtcttctcga gcggccggat catctcgagc
     5641 tcttcgcctg cgatctcgag cgcgcgcctg agcgtgacga gacgcgcctc ggcctcggcg
     5701 atctcctgcg tgcgctgccg cacttgtccg tcgagcgcgc cgagcgagcc gacgagctcg
     5761 cggcggcggg cttcgtactg gtcgcgctgg ctcgtgacga tatcgggacg ctgctcggca
     5821 agctcagccg gaaagacgac cggtcgatcc tcgacctcgg cctcgagccg ggcgatgatg
     5881 gccctgaggc ccagcatctt ctcgcgcacc tcgccgagcg aggagccggc gatcgtcggg
     5941 tcgatgcgca ggatcacgtc gccctcgcgc acaagcgcgc cttcgcgcac gaggatctcg
     6001 cgcacaatgc cggcctcgag gctctgcacc acctggatct tgctcgccgg gatgatgcgc
     6061 ccgcgccctg tcgtcacctc ctggacggta gcgatggcgg cccagccgat gaaagttccg
     6121 acgagggcga cgagcgcacc catcaggcat gtccaggcgc gctccggccg cgcaaggaga
     6181 tcggcgctca cggccgaagg tatcgcggca accggcgacg gcagtggcag agcgggcgca
     6241 gccgtcatgt cgcgcctccc ggctggaggg ccccgacacg aaccggagcc ttcgcccgtt
     6301 cgctggccgc gcgctcgctc atggcgcgca tggaggcgag caccgacgcc ttcgggccat
     6361 cgaggacctt gcgcccccca tcgagcacga tgatgcgctc gacgaggtcg aggagtgccg
     6421 gccggtgcgt gacgagcacc acagtcctgc cggcgagagc cgccttcagc cgcttcaccg
     6481 tctcgcgctc gctcgtcggg tccatggcgc tcgtcggctc gtcgagaagc acgactgtcg
     6541 gcttcccgac gatggcgcga gcgagcgtca ggctctgctt ctggccgctc gaaaggccct
     6601 ggccgcgttc cccgacacgc gtctcgagcc ccttcggcag ccgcatcacc caatcggccg
     6661 cagatgccgc ggcaagtgct gcgagaatct ggctgtcgct ccatcccgtc cgcccgagca
     6721 tgatgttctc gcgcagcgtc ccttggaaca cctcaccgtc ctggagcgcg agcccgacgg
     6781 acgctctgag aatcgccggc tcgatctggg ccgcgggcag cccgtcgacg aggacgcggc
     6841 ccgacgtcgg ctgtagcagc gcatcgatca gcttgagtgc cgtcgtcttg ccgctgccga
     6901 tgccgccaac gatcgccacc gcctcgcccg cggcaatcga gaacgacacg tcccggagcg
     6961 caggcggcgc gtctttctcg taggcgaccg tcacggcctc gaaggcaatg cttcccctca
     7021 cggcggacgg caggatgagg cgggcgtcgt cggtgcgttc ctgcggtgcg gtcatgatct
     7081 cgtcgagcat gcggtaggcg agctttgctt ggtgcagccg cgtgatgagc gccgccgcct
     7141 gtccgagcgg ctgcagcgcg cggccggcga gcatggtcgc cgcgatcagg ccacccatgg
     7201 tgaggctacc ggactggacc atgtagaacc cggcgatgat catgacgacc tgggcgaggg
     7261 tctgcacggc gccaacaagc cccatgccga tcgaggacag gtcgcgcagc gcgtggccgt
     7321 tgcgcacacc atcggcgacg gcacgctccc agcggcccgc agcaaagctt tcggcgccgg
     7381 cggccttcag cgtctcgagc ccgccgatcg tctcgacggc gaccgcgctc ttttgtgccg
     7441 aggcccgaaa cgcagtctcc gagaggcgca tgatgcgacg ctgcgttacc cacgcgatgg
     7501 cgagtacgat cgggatcgac agcagcggca cccagacgag gctgccggca acaactgaga
     7561 tcatcgccag gaagagtacc aggaagggca gatcgccaaa ggccgtcagc gtcgccgagc
     7621 tgaagaactc gcgcaccgtc tcgtactctc gaagcgtgtt tgcgcggaca cctgtcgagc
     7681 cacggacccc gagccgcgcg ccaaggaggc ggccgaagat gcgattggcg agcagcacat
     7741 cggcgcgccg gccgacacca tcgatgacgc gcgagcgcag ggtcttgatc gcgaagtcga
     7801 agagcgtcgc caggatgacg ccgatcgaca gggcccagag tgtctcgacc gcggcgttcg
     7861 gcaggacgcg gtcatagacg ttcattgtga agagcggcat cgcgagcgcg agcagattga
     7921 cggcaagcgt cgcaatgatc gcctggacgt agaggcgccg gctcgacttg aaggcctgga
     7981 gaagccaacc tggctgcggg gccggcggca atccatcggc tgccgctcgg gttacgctcg
     8041 gcggcctcac acgcagcacg cggccatcgt gtgccgctgc atattcagcg taggcgatgt
     8101 gcgagcgcgt gccaggtgca cccggctcgc tcacctcgac gtcgactggg cggccatcgc
     8161 cggcgcggcg gatcgaccag aggatgtcgg cgctgccgtc attgatgagc acgatcgccg
     8221 gcaggtcgag atcgcgaagc tcatcgagcc cgatgcttgc cgcctcgaga gtgagcgatg
     8281 cccgcaccgc ggcctcggcg gcatgctcga cggacagccg cccgccgacg agcggcaggc
     8341 cgcgcgtgat caccgtgacc gttgtcgcct cgccgtagtg ggccgccagg tcgacgatcg
     8401 cttgagcgag gcgatcgccc tccgcctcgc ccgccttcga cgatagcgcc atggcatnnn
     8461 nnnnnnncgc catttcgagg cggcgcagga cctccggtat ggccggggca ctctctggcg
     8521 acagtcgctc ggtcatggtg gcgctcgatc gctcgcctac ttgaccggct ttgccggagc
     8581 ggatggcacc ggcgacacat gataggtaac cgtaggccgc caattcgcga aaccgtcgcg
     8641 ccagccgtcg atgacggtgg gcgcatgcgg catcaccgcc tcacgcggcg gctcgaggcc
     8701 gagcgccggc acgaggcggc ccatggcggc gagcaggcgg tagctgttgt agaggcggac
     8761 gagctcctcc gtccgaagag cggcctcagc gagaaagacc tcggcctgga tgttgaggag
     8821 atcgagcagg cggcgctggc ccgcgtcgaa ctgggagccg taggcggcgc gcgtgaggcg
     8881 tgtctgatgc acctgcttga cgagcagcgg aatgcggctt ctcgcgatgt cgaccgcgtt
     8941 ccaggaggcg cgcgtctcgc gctcgacgat ccgcttcgtg ttctccgaga tctcgctcgc
     9001 ctcgagcgcg cgagcccgcg cttcccactc gcgcgccttg tcgatgccgc cgttgagaag
     9061 gttccagcgc accaccagca tcgcacgcgc gtcgatcgag cggtcgtgat cctcggcgac
     9121 gttccatccg tgattggaag aaagctcgag gttgagacgc ggataatggc gggaatgggc
     9181 gacaccgact gctgcgtgcg ccgccgtcgc gtcgtgcgcg gtcgcgatca cggagggagc
     9241 ggcggcgatg gcctcggcga ctgcagcatc gatcgagcgc ggcagatggt tcgccgggaa
     9301 ggagaccgac gcgagcttac ccggcgcccg gcccgtcacc gagcggaaca gggtctcggc
     9361 ctcccggagc cggccttccg cctcgagcgc gacggctgcg gcattggc
//
LOCUS       DAORNH010000175         9403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5407_length_9403_cov_2.722080, whole genome shotgun sequence.
ACCESSION   DAORNH010000175 DAORNH010000000
VERSION     DAORNH010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5407_length_9403_cov_2.722080"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>990
                     /locus_tag="PK264_16380"
     CDS             <1..>990
                     /locus_tag="PK264_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRD77484.1"
                     /translation="GSISREAHTTLAIAMNRIGGKSNTGEGGEEADRFKPLPNGDNMR
                     SAIKQVASGRFGVTTEYLVNSDVMQIKMAQGAKPGEGGQLPGHKVDATIAKVRHSTPG
                     VGLISPPPHHDIYSIEDLAQLIFDLKNTNPRADVSVKLVSEVGVGTVAAGVAKARADH
                     ITIAGFEGGTGASPLTSIKHAGSPWEIGLAETHQTLVYNRLRGRVAVQVDGGVRTGRD
                     VAIGAMLGADEFGFATAPLIALGCIMMRKCHLNTCPVGVATQDPVLRARFKGQPEHAI
                     NFFFFIAEEVREILASLGMRSLSELVGQSDILDKDRAIGHWKARGLDFGRLFH"
     assembly_gap    991..1018
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1019..2036
                     /locus_tag="PK264_16385"
     CDS             <1019..2036
                     /locus_tag="PK264_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRD77485.1"
                     /translation="GGVAIHNCERQDHGLDKVLDQKLIALAKPALEEKRPVKADIAIS
                     NRDRTAGAMLSGEVARRYGHAGLPEDTIWISFKGTAGQSFGAWTAHGVTLDLVGEAND
                     YVGKGLSGGKIIVRPDPTSGITPEESIIVGNTVLYGAITGECYFRGVAGERFAVRNSG
                     AVCVVEGTGDHGCEYMTGGVVVVLGSTGRNFAAGMSGGIAYVLDEDGSFDQKCNLAMI
                     DLAPVAAEEEVMRRLANQGGDLEAHGLVDVMADMTSQDAERLHALITRHAHYTNSAKA
                     KTILADWKSWAGKFKRIMPVEYRDALSKMAEHQKADSTGLDVIEIGMPKAKGKGKSRA
                     KPAG"
     gene            2108..2332
                     /locus_tag="PK264_16390"
     CDS             2108..2332
                     /locus_tag="PK264_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HRD77486.1"
                     /translation="MTNSQIVLVPKIKFGKPVNRGTPIPVELLLKLLSKGHTSEQLLE
                     SYPRLTRDDILAAQAFAAEHLPRADSTAAE"
     gene            2336..2707
                     /locus_tag="PK264_16395"
     CDS             2336..2707
                     /locus_tag="PK264_16395"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HRD77487.1"
                     /translation="MRFLVDECLLAEIVNELRLDGHDVHWVADDLQSVEDAVLLEIAN
                     AERRILMSDDRDFGELVFKCAMPDIGIVSLRVAKFKMDAGRIGAYAADKVAELGPLLL
                     GQFALIEPGGVWLRSLPRSLA"
     gene            2750..3346
                     /locus_tag="PK264_16400"
     CDS             2750..3346
                     /locus_tag="PK264_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD77488.1"
                     /translation="MIIHGDIGSGNCLKVKWTADHLRLPYTWVAVDIMKGEAKTPAFL
                     AMNPAGQTPTVDFGNGRFLAQSNAIIRHLARGSALLPDDAWLQAKADEWLFWEQYSHE
                     PYVAVCRFHMHYLGKPKETREAWRVERGEKALDLMERQLTGKSFLVGERLTIADIALL
                     AYTRVAGEGGFDLMPRPAIRAWIARCENLLSLGMGSAP"
     gene            complement(3318..3719)
                     /locus_tag="PK264_16405"
     CDS             complement(3318..3719)
                     /locus_tag="PK264_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRD77489.1"
                     /translation="MSSEIFLDTNVIVHLFAREAEKRLRSEALIADGATISVQVLNET
                     ALTLRRKLKMSWAAIADVSSRLRDVCDVVPLTEATHVRGLAIATRHQLHVYDSMIVAA
                     ALLAGCSTVHSEDLHDGLVIEGLTVLNPFRD"
     gene            complement(3709..3942)
                     /locus_tag="PK264_16410"
     CDS             complement(3709..3942)
                     /locus_tag="PK264_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRD77490.1"
                     /translation="MQVSKWGNSLAIRLPKAVVDELELKEGDEVEIIGATREKIEIAR
                     RDARIQVLKGLRKHGERLPAGYKFTRKDAYDEF"
     gene            4049..5515
                     /locus_tag="PK264_16415"
     CDS             4049..5515
                     /locus_tag="PK264_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRD77491.1"
                     /translation="MGKITGFLEHDRKDRAYAPVAERVKHWAEFVQPLPEKEVRTQAG
                     RCMDCGIPYCHSGRPGQTHGCPVNNQIPDWNDLVYRGHWQEALRNLHSTNNFPEFTGR
                     ICPAPCEASCTLNIEDQPVTIKTIECAIIDRGFAEGWVKPQLADTKTGKRVAIIGSGP
                     AGLAAAQQLARVGHEVHVYEKSGFPGGLLRYGIPDFKLEKAVVARRIKQMEVEGVQFH
                     CGVHVGKAVTMKELEGKHDAVLLAMGSEQPFDLFARMPGRDLAGLHFAMDFLPQQNRR
                     VAGEKQPKGTAEISAKGKNVIVIGGGDTGSDCIGTSIRQGAKSVTQLEIMPEPPKKEN
                     KALAWPHWPLKLRISSSQAEGAKTEYSISTTGFVGEGGRVTALTYTRVDGKLQPVPGS
                     AGKLEADLVLFAMGFGGPVESDVIRELAVPVVPRGRFKGVDANEKDYRVAGAASGKLY
                     AAGDVRRGQSLVVWAIREGRQAAAAIDKALMGKTVLPR"
     gene            complement(5537..6475)
                     /locus_tag="PK264_16420"
     CDS             complement(5537..6475)
                     /locus_tag="PK264_16420"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRD77492.1"
                     /translation="MAGAPLGIMITAVAGVTSLGLLAPVVPMADAINHARPLLLFAAL
                     ALAAVAWQVGTRLQRTAAGLAAAVNVALAAAPLLAVAPLSERSAQTFKVISFNVWIHN
                     RNIAAIASFLRKEAADIVVLQELHAVVADVLMDKVRDLYPHAIHCTGLRACDGAILAR
                     RPWRDAHLMLRVAPRPPSVHARFEDDGAGGPLRVIGTHLARPDKPRLQAEQLHALIAD
                     IGAPGGPDDVPTVVAGDFNLTPWSWHLNVLSRQTGLMRPVTGGASWPAHRTTDEPMVL
                     IDHVLVSRHFAVVSYRTGPRLGSDHLPVIVEFARRP"
     assembly_gap    6530..6642
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6643..7464)
                     /locus_tag="PK264_16425"
     CDS             complement(<6643..7464)
                     /locus_tag="PK264_16425"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRD77493.1"
                     /translation="MQIMDVLRQAQGGQAIDNLASAFSVEPEQADSVVAVVLPELAQR
                     LERITLSRGGLADLVKALGDPRHRRALVDPGVFTDPAVGADGNAILGHIVGTRDASRA
                     IAARAADASGLDEETVKRMLPFVAAFAMSGIAASLSGQLGERGYGAPGGSSGVNDGGQ
                     MAGPGNNGARPGLSRERRPGTAAGSSPHRSAVRRLFAPASRRRTQLLAPAHRRRGATD
                     GARLQWLTLAWRRRLAAGRADALARLVVRSRRRPADTARSQHPRRHLGYDPARRPI"
     gene            complement(7561..8172)
                     /locus_tag="PK264_16430"
     CDS             complement(7561..8172)
                     /locus_tag="PK264_16430"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HRD77494.1"
                     /translation="MPENEDGGYRSRLKSRMVEIARGLLETEGLMAVQARRVTGMAEC
                     SVGTLYNVFNGLDGLIITVNTQTLKELGGALDAALAESRGQSIGERLMALAQSYLDFA
                     EAHPKRWKAIFEHRFMSSKAAETMLGPYRADQNELFKLVEALLEGPMPNGMDRPNLAR
                     ALFAAVHGIVMMALDEKLGNYNRDETLSRIRFIISAGVVALKR"
     gene            complement(8357..>9403)
                     /locus_tag="PK264_16435"
     CDS             complement(8357..>9403)
                     /locus_tag="PK264_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRD77495.1"
                     /translation="IDGLLHVTDMAWRRVNHPSDVVAVGDRVKVQIIRINPDTQRISL
                     GMKQLQSDPWQGIEAKYPVGARYKGTVTNIADYGAFVELEPGVEGLIHVSEMSWTKKN
                     VHPGKIVSTSQEVNVQVLEVDPVKRRISLGLKQTQDNPWDVFLASHPKGSVVEGPIRN
                     TTEFGLFIGLDGGVDGMVHLSDLDWNRSGDEVIKEFKKGDMVKAVVLDVDPQKERISL
                     GIKQLAGDPLDTIGKYKKGDPVTCEVVTVEDGGITVKLADTDVTSFIKRNDLSRDRSE
                     QRPERFNVGDKVDAQVQTVDRSSRRVTLSIKALELAEEKQAVAQYGSSNSGATLGDIF
                     KATIKKREGDEPGA"
BASE COUNT         1661 a         3039 c         3026 g         1536 t
ORIGIN      
        1 tcggctcgat cagccgcgag gcgcacacca cgctcgccat cgccatgaac cggattggcg
       61 gcaagtccaa tacgggcgaa ggcggcgagg aggccgatcg cttcaagccg ctgccgaacg
      121 gcgacaacat gcgttcggcg atcaagcagg tcgcctcggg ccgcttcggc gtgacgacgg
      181 agtaccttgt caactccgac gtgatgcaga tcaagatggc gcagggcgcc aagcccggcg
      241 agggcggcca gctgcccggc cacaaggtcg atgcgaccat cgccaaggtg cgccactcga
      301 cacccggcgt cggcctcatc tcgccgccgc cgcatcacga catctactcg atcgaggatc
      361 tcgcgcagct gatcttcgac ctgaagaata cgaacccgcg cgcggacgtc tcggtgaagc
      421 tcgtctccga ggtcggcgtc ggcacggtcg cggcgggtgt cgccaaggcg cgcgccgacc
      481 acatcacgat cgccggcttc gagggcggca ccggtgcctc acccctcacc tcgatcaagc
      541 atgcgggctc gccctgggag atcggcctcg ccgagacgca ccagacgctt gtctacaacc
      601 ggctgcgcgg gcgcgttgcc gtgcaggtcg acggcggcgt gcgcacgggc cgcgatgtgg
      661 cgatcggcgc catgctcggc gccgacgagt tcggctttgc gacggcgccg ctgatcgcgc
      721 tcggctgcat catgatgcgc aagtgccacc tcaacacctg cccggtcggc gtcgcgaccc
      781 aggacccggt gctgcgcgcg cgcttcaagg ggcagcccga gcacgcgatc aacttcttct
      841 tctttatcgc agaggaggtg cgcgagatcc tcgcgagcct cggcatgcgc agcctgagcg
      901 agctcgttgg ccagtccgac atcctcgaca aggaccgcgc gatcggccac tggaaagccc
      961 gcggcctcga cttcggccgc ctgttccaca nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     1021 ggggcgtcgc gatccacaat tgcgagcgcc aggaccatgg cctagacaag gtgctcgacc
     1081 agaagctgat cgccctcgcc aagcctgcac ttgaggaaaa gcgccccgtc aaggcggaca
     1141 tcgccatctc gaaccgcgac cggacggcag gcgccatgct gtccggcgag gtcgcgcgcc
     1201 gctacggaca cgcggggcta ccagaggaca cgatctggat cagcttcaag ggcacggccg
     1261 gccagagctt cggcgcctgg accgcgcacg gcgtgacgct cgatctcgtc ggcgaggcca
     1321 acgactacgt cggcaagggc ctctcgggcg gcaagatcat cgtgcgaccc gacccgacct
     1381 cgggcatcac gcccgaggag agcataatcg tcggcaatac ggtgctctac ggcgcgatca
     1441 ccggcgagtg ctacttccgc ggcgttgccg gcgagcgttt cgcggtgcgc aactcgggcg
     1501 ccgtttgcgt cgtcgaaggc acgggcgacc atggctgcga gtacatgacc ggcggcgtgg
     1561 tcgtcgtgct cgggtcgaca gggcgcaact tcgcggccgg catgtcgggc ggcatcgcct
     1621 acgtgctcga cgaggacggc agcttcgacc agaagtgcaa cctcgccatg atcgacctcg
     1681 caccggtcgc ggccgaggaa gaggtgatgc gccgcctcgc caaccagggc ggcgacctcg
     1741 aggcgcacgg cctcgtcgat gtaatggccg acatgacctc gcaggacgcc gagcggctgc
     1801 atgccctcat cacgcggcat gcccactaca cgaactcggc aaaggccaag acgatcctcg
     1861 ccgactggaa gagctgggcc ggcaagttca aacgcatcat gccggtcgaa taccgagacg
     1921 cgctctccaa gatggccgag catcagaagg cggattcgac cggcctcgac gtcatcgaga
     1981 tcggcatgcc gaaagcgaag ggcaagggca agtcgcgcgc gaaaccggca gggtgacatg
     2041 tcattagcgc cgagcaccgt ggcacgtaga cttcgtgcta agttattgaa actcagcgag
     2101 gttgaccatg acgaactcgc aaattgtcct ggttccgaaa ataaagttcg ggaagccggt
     2161 caacaggggg acgcccatcc ccgtcgagtt gcttctgaag ctgctctcca aggggcatac
     2221 gagcgagcaa ctactcgagt cgtacccgcg gctcactcgc gacgatattc tcgcggcgca
     2281 agcttttgcc gcggagcatc ttccgcgggc cgatagcacc gcggcagaat gacggatgcg
     2341 atttctggtc gacgagtgct tgttggccga aatcgtgaat gagctccggc tcgatgggca
     2401 tgacgtccac tgggtggcag acgatcttca atctgtcgaa gacgcggtcc ttctggagat
     2461 cgcgaatgcg gagaggcgca tcctcatgag cgacgaccgg gatttcggag aacttgtctt
     2521 taaatgtgcg atgccggaca tcgggattgt gagcctgcga gtggcgaaat tcaagatgga
     2581 tgccggcaga attggcgcct atgccgccga caaagtggcg gaactcggac ctctgttgct
     2641 cgggcagttt gcgctaatcg agcccggcgg tgtctggctg cgcagcctac caagatcttt
     2701 ggcgtaacgt catgtggcag gcacaatcca gaatccgagc aatccaacaa tgataatcca
     2761 cggcgacatc ggctccggca actgcctcaa ggtgaagtgg accgcggacc acttgcgcct
     2821 cccctacacc tgggtggcgg tcgacatcat gaagggcgaa gccaagacgc cggcatttct
     2881 cgccatgaac ccggccggcc agacaccgac ggtcgatttc ggcaatgggc gctttctcgc
     2941 ccagtcgaat gccatcatcc gccatctcgc ccgcggctcg gcgctgcttc ccgacgatgc
     3001 ctggctgcag gcgaaggccg acgagtggct cttctgggag cagtacagcc acgagcccta
     3061 tgtcgcggtg tgccgcttcc acatgcacta tctcggcaag ccgaaggaga cgcgcgaggc
     3121 gtggcgcgtg gagcggggcg aaaaagcgct cgacctaatg gagcgacagc tcacgggaaa
     3181 aagcttcctc gtcggcgagc gcctgacgat cgcggatatt gcgctcctcg cctacacgcg
     3241 agttgccggc gagggtgggt tcgacctgat gcccagaccc gcgatccggg cctggatcgc
     3301 acgctgcgag aacctgctca gtctcggaat gggttcagca ccgtgagccc ttcgatgacg
     3361 agaccgtcgt gcaggtcctc gctgtggacc gtgctgcatc cggccaagag tgccgctgcc
     3421 acgatcatgc tgtcatacac atggagctgg tggcgcgtgg caattgcgag cccgcggacg
     3481 tgggtggcct cggtcagagg taccacatca cacacatcgc ggaggcgaga ggatacgtcg
     3541 gcaatggcgg cccagctcat cttgagcttt cgacgcaaag tgagcgcagt ctcgttgagc
     3601 acttgcacac tgatggtcgc gccatcggca atgagtgcct ccgagcgtag cctcttctcc
     3661 gcctcgcgtg cgaacaaatg cacgatgaca ttcgtatcga gaaaaatctc agaactcatc
     3721 gtaggcgtct tttcgggtga acttgtagcc ggcaggcaag cgctcgccgt gcttgcgaag
     3781 tcccttgagc acctgaatcc gtgcgtcgcg cctggcgatc tcgatcttct cgcgggtcgc
     3841 gccgatgatt tcgacctcgt cgccctcctt caactccagc tcgtcgacga cggccttggg
     3901 caggcgaatg gcgagactgt taccccattt cgaaacctgc acggcaacct cccatgatat
     3961 acaatcgcca ctaaagtata tcatcgacca acgaccgaac gcaagctccc tcgcaagccc
     4021 aaccgcagcg aagatcagga cgagtcccat gggtaagatc accggatttc tcgagcacga
     4081 ccgcaaggac cgcgcttatg cgccggtcgc ggagcgcgtg aagcattggg cggagttcgt
     4141 tcagccgctc cccgagaagg aggtgcgcac gcaggcgggg cgctgcatgg actgcggcat
     4201 tccctactgc cacagcggca ggccgggcca gacgcatggc tgcccggtca acaaccagat
     4261 ccctgattgg aacgaccttg tctaccgagg gcactggcag gaggcgctgc ggaacctcca
     4321 ctcgaccaac aacttccccg agttcaccgg tcgcatctgc ccggcaccgt gcgaagcgtc
     4381 gtgcacgctc aacatcgagg accagcccgt caccatcaag acgatcgagt gcgcgatcat
     4441 cgatcgcggc ttcgccgagg gctgggtgaa gcctcagctg gccgatacga agactgggaa
     4501 gcgcgttgcc atcatcggct cggggccggc aggattggcc gcggcccagc aactcgcccg
     4561 cgtcgggcat gaagtgcacg tctacgagaa gagcggcttc ccgggcgggc tcctccgcta
     4621 cggcatccct gatttcaagc tcgagaaggc ggtggtcgct cgccgcatca agcagatgga
     4681 ggtcgagggc gtgcagttcc attgcggcgt gcatgtcggg aaggctgtca cgatgaagga
     4741 gctcgagggg aaacacgatg ccgtgctgct cgccatgggc tccgagcagc cgttcgatct
     4801 cttcgccagg atgcccggcc gcgacctcgc aggactgcat ttcgcgatgg atttcctgcc
     4861 gcagcaaaac cggcgtgtcg ctggcgagaa gcagccgaag ggcaccgccg agatcagtgc
     4921 gaagggcaag aatgtcatcg ttatcggcgg cggcgacaca ggctccgact gcatcggcac
     4981 gtcgatccgc cagggcgcca agtcggtgac gcagctcgag atcatgcccg agccgccgaa
     5041 gaaggagaac aaggcacttg cctggccgca ctggccgctg aagctgcgca tctcctccag
     5101 tcaggccgag ggcgccaaga ccgagtactc gatctcgacg acgggcttcg tcggcgaggg
     5161 aggccgggtg acggcgctca cctacacccg cgtcgatggc aagttgcagc ccgtcccggg
     5221 cagcgccggc aagctcgagg ccgatctcgt gctgttcgcg atgggcttcg gcgggcctgt
     5281 cgagagcgac gtgatccgcg agctggcggt gccggtcgtg ccgcgcggcc gcttcaaggg
     5341 cgtcgatgcg aacgagaagg actatcgcgt cgcgggcgcc gcgtcgggca agctctacgc
     5401 cgctggcgac gtgcgtcgcg ggcagagcct cgtcgtttgg gcgatccgtg agggccgtca
     5461 ggcggcggcc gccatcgaca aggcgctgat ggggaaaacc gtgctcccgc ggtgaccgga
     5521 agcgggcgcg cttgcctcaa ggccggcgcg cgaactcgac gatgaccggc aggtgatcgg
     5581 agccaagccg cggcccggtg cgatacgaga cgaccgcgaa atgccgcgag acgaggacgt
     5641 gatcgataag caccatcggc tcgtccgtgg tgcgatgcgc cggccagctc gcgccgcccg
     5701 tgacgggccg catcaggccc gtctgccggc tcaacacgtt caagtgccaa gaccacggcg
     5761 tcaggttgaa atcgccggcg accacggtcg gcacatcgtc gggcccgccc ggcgcaccga
     5821 tgtcggcaat cagggcgtgc agctgctccg cctggaggcg cggcttgtcc ggacgggcaa
     5881 ggtgtgtgcc gatcacgcgc aacggccccc cggcgccgtc gtcctcgaag cgggcgtgca
     5941 ccgagggcgg ccggggtgcc acccgaagca tgagatgagc gtcgcgccag ggccgccgcg
     6001 caaggatcgc gccgtcacag gccctcagcc ccgtgcagtg aatggcgtgc ggatagaggt
     6061 cgcggacctt gtccatcagc acgtcggcga cgaccgcatg cagctcctgc agcacgacaa
     6121 tgtcggccgc ctccttccgc aggaaggacg cgatcgcagc gatattgcgg ttgtggatcc
     6181 agacattgaa cgagatgacc ttgaacgtct gcgcacttcg ctcgctcaaa ggcgcgacag
     6241 ccagcagtgg tgctgccgcc agcgcgacat tgacggctgc ggcaaggccc gccgcggtac
     6301 gctgcaggcg cgtgccgacc tgccaggcca cggcggcgag cgccagcgct gcgaacaaca
     6361 gaagcggtcg cgcatgattg atcgcatcgg ccatgggcac gaccggcgcc aggaggccga
     6421 gcgaagtcac ccccgcaaca gccgtgatca tgatgccgag cggtgcgcct gccatccctc
     6481 agcgacccgt gaatgcccgc ccgatgatgc gcttcagaat gctccagccn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngattggcc gccgcgccgg
     6661 atcgtatccg agatgtcgtc gagggtgctg cgacctggcg gtgtcagcgg gccgccgccg
     6721 ggaccggacg acgagccggg ccaacgcgtc tgcccgcccg gctgcaagcc gccgccgcca
     6781 ggccagggtg agccactgga gtcgggcgcc atcggtcgct cctcgccgcc gatgggctgg
     6841 ggcgagtagc tgcgtccgtc gacgggaggc gggagcgaaa agccgccgga cggcggaccg
     6901 gtgcggggag gagccggctg cggtgccggg acgccgctcc ctggaaagcc cgggccgcgc
     6961 accattattg ccagggccgg ccatctggcc gccgtcgttc acgcccgacg agccgccggg
     7021 cgccccatat ccgcgctcgc cgagctggcc tgagaggctc gctgcgatcc cgctcatggc
     7081 gaaggccgcc acgaacggca acatgcgctt cactgtctcc tcgtcgaggc ccgaggcatc
     7141 ggcggcgcgg gctgcgatgg cgcggctcgc gtcccgcgtg ccgacgatat ggccgaggat
     7201 ggcgttgccg tcggcgccca cggccggatc ggtgaagacg cccggatcga cgagcgcacg
     7261 cctgtgccgg ggatcgccca gcgccttgac gagatcggcg agcccgccgc gcgagagcgt
     7321 gatgcgctcg aggcgctgcg caagctcggg gagcaccacc gcaacgacgc tgtcagcctg
     7381 ctccggctct acactgaacg ccgatgcgag attgtcgatc gcctggcccc cctgggcctg
     7441 gcgcagaacg tccatgatct gcataggtct gtttgtccgc cgatatccgt ccggctcatc
     7501 caatagcaga cgacaccccg ccttgtcgcg gggcctccgg ctcctgctct cgtgtgcgtc
     7561 ctaccgtttg agcgccacta ctccagcaga gatgatgaag cggatacgcg aaagcgtctc
     7621 gtcgcggttg tagttgccga gcttctcgtc gagcgccatc atgacgatgc cgtgcacggc
     7681 cgcgaacagc gcccgcgcca ggttcgggcg gtccatgcca ttcggcattg gcccctcgag
     7741 cagcgcctcg accagcttga agagctcgtt ctggtcggcc cgatagggcc cgagcatcgt
     7801 ttcagccgcc ttgctcgaca tgaagcggtg ctcgaagatc gccttccagc gcttcgggtg
     7861 tgcttcggcg aaatcgagat aggactgcgc gagcgccatc agtcgctcgc cgatcgactg
     7921 gccgcgcgac tccgccaggg ccgcatcgag tgcccctccg agctccttca aggtctgcgt
     7981 gttgaccgtg atgatcaggc catcaaggcc gttgaatacg ttgtagagcg ttccgacaga
     8041 acactcggcc atgcctgtga cgcgccgtgc ctggacagcc atgagaccct cggtctcgag
     8101 cagcccccgg gcaatttcca ccatgcgcga tttcagccgt gacctatacc ccccgtcttc
     8161 attctcgggc atcacctcct ccagggtgcg tcgcaatttg cgatctttgg cagcattcac
     8221 atcgtatacg ccggaccgtc actcggtcta tgctcaacta cgaagcaaat cgaggaatga
     8281 tacacctcga atatgcgaaa agggggcacg agtgccccct ttccctgcat gattgtcttg
     8341 tcgcgaacca atccgctcag gcgcccggct cgtcgccctc gcgcttcttg atcgtcgcct
     8401 tgaagatgtc gccgagcgtc gcgcccgagt tcgatgatcc gtactgggcg acggcctgct
     8461 tctcctcggc gagttcgagc gccttgatcg acagcgtcac gcggcgcgag ctgcgatcca
     8521 ccgtctgcac ctgggcgtcg accttgtcgc cgacattgaa gcgctcggga cgctgctcgg
     8581 agcggtcgcg cgagaggtcg ttgcgcttga tgaagctcgt cacgtctgtg tcggcgagct
     8641 tcacggtgat gccgccgtcc tcgaccgtga cgacctcgca ggtgacgggg tcgcccttct
     8701 tgtacttgcc gatcgtgtcg agcggatcgc cggcgagctg cttgatgccg agcgagatgc
     8761 gctccttctg ggggtcgacg tcgaggacga cggccttgac catgtcgccc ttcttgaact
     8821 ccttgatgac ctcgtcgccc gaccggttcc agtcgaggtc ggagaggtgc accatgccgt
     8881 cgacaccgcc atcgaggccg atgaatagac cgaactcggt ggtattgcgg atcggtccct
     8941 cgacgaccga gcccttgggg tggctcgcga gaaacacatc ccacgggttg tcctgggtct
     9001 gcttcagacc gagcgagata cgccgcttaa cggggtcgac ctcgagcacc tggacattca
     9061 cctcctggct cgtcgagacg atcttgccgg ggtggacgtt cttcttggtc cagctcatct
     9121 cggagacgtg gatcaagccc tcgacgcccg gctcgagctc cacgaaggca ccgtagtcgg
     9181 cgatgttcgt gaccgtgccc ttgtagcgcg cgccgaccgg gtacttggcc tcgattccct
     9241 gccagggatc ggactgcagc tgcttcatgc cgagcgagat gcgctgtgta tccgggttga
     9301 tgcggatgat ctgcaccttc acgcggtcgc cgacggcgac gacgtcgctc ggatggttga
     9361 cccggcgcca ggccatgtcg gtgacgtgca ggaggccgtc gat
//
LOCUS       DAORNH010000176         9399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5413_length_9399_cov_2.377568, whole genome shotgun sequence.
ACCESSION   DAORNH010000176 DAORNH010000000
VERSION     DAORNH010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9399
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5413_length_9399_cov_2.377568"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PK264_16440"
     CDS             complement(<1..784)
                     /locus_tag="PK264_16440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77496.1"
                     /translation="MMDRCNVCGGSLRSIAAYAALPRVSSDCKPVAPGGSVAACSACG
                     GIQKPVTSAFLADIGVIYAKYDVYYQGGGMEQIVFDSRKGAPSRRSELLSDRLLSTDL
                     LPAKGSVVDLGCGNGAFLASLAARLPGWSLAGLELDRKHEATLARIPRFERLIVGEPE
                     ALSGSFDLISMIHALEHFTDPLRTLTAIRARLTPQGLLFIEIPNVEQNPFDLLIADHV
                     SHMTPWTLETLLARAGFEVVHLATDWVGKELSVLARPAPSPPA"
     gene            complement(781..2016)
                     /locus_tag="PK264_16445"
     CDS             complement(781..2016)
                     /locus_tag="PK264_16445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00180 family glycosyltransferase"
                     /protein_id="HRD77497.1"
                     /translation="MAAALTIVIPTYNRPADLGRLVRYIGTAIPAARAVVLDSSRGDA
                     ATGARSVCLAHPTVTYRSYPPEARFYEKLADGIDRFVDTDAVCLCADDDLLVGPGILD
                     AARVIETDQTVAVAHGYYAQFTLHGGGGRLTGLGTIAPASTSSDPMARIAEGLLSYEA
                     TLYAVHRRELLIDILKEASSAPSLLSAELLTCVLALSAGRMHRVQAFTHARSIAPSHG
                     YSHWHPAEALAVDPARLGAGLAFVRSRLLARHPELPDTRLRVFDLALFAYVASYVQPG
                     TARRIARMAIEGAGDQQRLDVGWSAFTASLQPTRSMSAVRGSAPARWVKSRLAGHPEI
                     RERLLRLMRNGARHVPTEGVKLADGRLTTIALEAGFTERLASIPLAIDAPATRSLVEA
                     MAIYSEGLLKPAPKFPEGP"
     gene            complement(2016..2717)
                     /locus_tag="PK264_16450"
     CDS             complement(2016..2717)
                     /locus_tag="PK264_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77498.1"
                     /translation="MLDTVTAERAPDLEQLIHTGRMVKLRQLPIELIENAVDPVKLVH
                     AAAVGGRSAVLAHLARLGLKVDHVAVLCQGVLGDQVPTMVYLEERGIATCTPIAVSIA
                     LPHDRGRVMRHWLERGLVEDPVSIAHQAIHHDALYCFRALEQHPKVKADSSLLDYARG
                     GSKPRIRQYLNPDLLNSAAPEPAAASASVPAAAATGQPRYTIMTPSEHIDPDKTTIAF
                     LLYRRFMRMLFVRSR"
     gene            3010..>4024
                     /locus_tag="PK264_16455"
     CDS             3010..>4024
                     /locus_tag="PK264_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77499.1"
                     /translation="MQPGRQALIDRRPPAMRALVDAAKQGNARRVSELLDDPSAAPYV
                     TWAAYAAAATGGVAVTKVIAEKCGDATLHDREHLALRLSARRGDLDMFKMLLDTGSLL
                     AAKDRQALRLAALHRHDAIISEGWRRGFPLWTIEELVAAARADRVDRVRWILSDPRFT
                     EQSWRVAFAAVDAGAVGVIRMLAATRPSALHDRNHLALRMAARAGRLDVFQALLGSGS
                     EPAARGGQALALARLHGHRRIARICENRGAGGFAPATVRDLLDAACSGDTARVDELID
                     DPIFEQHASRVGIAAAAGGAVEVVELLIRRLSAQVLHHRDHLALRIAARRGDLAIVDV
                     LLAN"
     assembly_gap    4025..4072
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4073..5876
                     /locus_tag="PK264_16460"
     CDS             <4073..5876
                     /locus_tag="PK264_16460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HRD77500.1"
                     /translation="RRHDDVIERLVEAGVPIFRKDELSNPFDEIRRRASGPVVFLHIA
                     KTAGSSIGSMLMRTFPVAEIVEYDRAQAMAEPLSPSYCWHRQPVAKALGALTPAEQSA
                     PRLLAGHFSYRIREVLPPSRLYISMIRHPIDRLVSGLYYGSGVGKRPTTIDDFRLLYD
                     WDYEIQPTDRYLYMDSQVRTLSGDPSLDPDKLGSENAFRAVTDDVTDQVLAEAAENFI
                     LLGITERYEESMIVLARQLGVPLTLIIDRPRNRTEHRPRLDEVPAGAITALTNYNRQD
                     LRLYAGANRLLDRIIEEDRSGFERDREILARLKEKHAAKEGYPRLLAYERAARGMPPP
                     RLFYPEAPPRRPLDADRASAAYLDLMEDVLVGRVYQDPSLAPWNRGRYMAQDRQIGSD
                     WPSRAHSMIGKQRMHNVRMLCEQAVREGVRGDFLEAGVWRGGATIMMRAVLHAHADRS
                     RTVWVADSFTGVPPPDEQTYPADTGDKHHAAADVLAISQAEVASNFRSYGLLDRQVRF
                     LPGLFKDTLAHAPIERLSVLRLDGDLYESTMDTLVALYDKVSPDGFIIVDDYGAVPGC
                     KAAIEEFRKRRAIAAELVAIDWAGVYWRKPRTAI"
     gene            5908..6723
                     /locus_tag="PK264_16465"
     CDS             5908..6723
                     /locus_tag="PK264_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77501.1"
                     /translation="MATTSTSPAITFVIDALRHVPLIRVLALNHLREKFIGTWAGIVW
                     AFIHPIVLIAIFWVVFAQGFKLPTSGERPFLLVLICGLLPWMAFNEAVMGAAGSVTSK
                     AYLVRKIAFPLEVLPLTNVVSSLIVHVPMLLLGMIIAAWHGRFPGPHLAWLPVYMLAL
                     ALLATGLGMLLSALSVVFRDVQQGLGIVMNIWFWATPIVWAEDMLPAEYRWLSQWNPI
                     TYLIDGYRYALLGPGVPEPTTFAAVLYWSVTGVVMAAGILLFKRLKPNFADLL"
     assembly_gap    6918..7062
                     /estimated_length=145
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7320..>9399
                     /locus_tag="PK264_16470"
     CDS             7320..>9399
                     /locus_tag="PK264_16470"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD77502.1"
                     /translation="MCVLCGDVWHVSSDSYRFSPSATTSGGSGSSSIYALAENGTEGG
                     GSFVGSGVPMPGISGASGPGSSALLAQTFSGGAFHFSGDRNVDAALIGSRWTFLNHTY
                     SFPTSGTFYVGYTTGDNTTGFSTFNAAQQAGVVYAYGLVSQYTPMTFTPITETATTHA
                     THRFANTTNVGSAYGNFPSDNFAAGDSWYNLGQPFYSNPQVGNWGLATIMHEIGHTLG
                     LKHGHQDYTALDLSGYLFVVGPRFGSRALESTKDGQPYSLMTYSGGVGIPPTSFQGDG
                     FNQPQTYMMYDIAALQYMYGANYASSGQTFSGNSRYTFNSFSGAMYINGVPEGSPPTS
                     NIVFRTVWDGHGNDTYDLENYTTPMSLNLEAGGWLVFDTDSTGGFLQRANNQPLSGGP
                     VWAPGNVANALLYQNNVASLIENAIGGTAADTIIGNRVSNILDGGDGNDSIYGAGGDD
                     FLYGREGADTLYGDASPAGNSGISFGTGLVTTRGTSIATAVNVTATFSLANNADIANS
                     TTVPHTTGRFTTAASGTVVASYYAIVLNAGSTITLDVDRTSGALDSWIRFYNSAGTVL
                     AQNDDAGGDAGSTGVTIDSALTYSITATGTYYFSIGQYSGGAYLDTLPNSVTYDLNVS
                     VEAPAAIGNDGAAGNDILNGGPGGDQLFGGTGFDYASYATAVAGVAAFLTVPGSNTGE
                     AAGDSYNSIEG"
BASE COUNT         1583 a         2989 c         3018 g         1616 t
ORIGIN      
        1 tggctggcgg gctcggtgcg gggcgcgcga gcaccgagag ctccttcccg acccaatcgg
       61 tggcgagatg cacgacctcg aagccggccc gggcgagcag cgtctctagc gtccacggcg
      121 tcatgtgcga gacatggtcg gcgatgagca ggtcgaaggg gttctgctcg acgttcggaa
      181 tctcgatgaa gaggaggcct tgcggcgtga ggcgcgcacg gatcgccgtc agcgtgcgca
      241 gcgggtcggt gaagtgctcg agcgcatgga tcatgctgat gagatcgaag gagccggaaa
      301 gcgcctccgg ttcgccgacg atcaggcgct cgaagcgcgg tatgcgggca agcgtcgcct
      361 cgtgcttgcg atcgagttcg aggccggcga gcgaccagcc cggcaggcgc gcggcaagcg
      421 aggcgaggaa ggcgccattg ccgcagccga gatcgaccac ggagcccttc gccgggagca
      481 ggtccgttga caggaggcgg tccgagagca gctccgagcg tcgagatggc gcgcccttgc
      541 ggctgtcgaa gacgatctgc tccatgccgc cgccctgata gtagacatcg tacttggcat
      601 agatcacccc gatgtcggca aggaaggcgc tggtcacagg cttctggatg cctccgcacg
      661 cggagcaggc cgcaacgctg ccgccgggcg ccacgggctt gcagtcggaa ctcacgcgcg
      721 gcagggccgc gtaggcagcg atcgatcgaa gcgacccgcc gcagacgttg caccgatcca
      781 tcatgggccc tccggaaact tcggtgccgg ctttagcaga ccttcggagt agattgccat
      841 ggcctcgacc agcgatcggg tggccggtgc atcaatcgcc agtggaatac tcgcgagacg
      901 ctccgtgaaa ccagcctcga gggcaatggt ggtcaaccgt ccatcggcaa gcttcacgcc
      961 ctccgtcggc acgtgacgcg cgccattgcg catgagccgc agcagacgct cgcggatctc
     1021 agggtgcccg gcgagcctcg actttaccca gcgggccggt gcggagccgc gcacggcgga
     1081 catggaacgt gtcggctgca ggcttgcggt gaaagcgctc cagcccacat cgagccgctg
     1141 ctggtcgccg gcaccctcga tcgccatgcg cgcaatccgg cgcgccgtcc cgggctgcac
     1201 ataggaggcg acgtaggcaa aaagagcgag atcgaagacg cgcagccgcg tatcgggaag
     1261 ttcggggtgg cgtgccagca gccgggaacg gacgaaagcg agtccggcgc caaggcgggc
     1321 cgggtcgacg gcgagcgcct ccgcgggatg ccagtgcgaa tagccgtggg agggcgcgat
     1381 gctgcgcgcg tgcgtgaagg cctgaacgcg gtgcatgcgt ccggccgaaa gggcgaggac
     1441 gcaggtgagt agctcggctg agagcaggct cggagcgctc gaggcctcct tcagaatgtc
     1501 gatgagcagc tcacggcgat gcacggcata gagtgtcgcc tcgtagctga gcaggccctc
     1561 ggcgatgcgc gccatggggt cgctgctggt gctcgccggc gcgatggtgc cgaggcccgt
     1621 cagtcggccg ccaccgccgt gcaacgtgaa ctgcgcatag tagccgtgtg cgacggcgac
     1681 ggtctgatcc gtttcgatca cgcgagctgc gtcgaggatc ccggggccaa cgagcaggtc
     1741 gtcgtcggcg cagaggcaga ccgcgtcggt atcgacgaaa cggtcgatgc cgtcggcgag
     1801 cttctcgtag aagcgggctt cgggcggata ggaccggtag gtgacggtcg gatgcgccag
     1861 gcacacggat cgggcgccgg tcgccgcgtc gcctcggctc gaatcgagca cgactgcgcg
     1921 tgcggcggga atggctgtac cgatgtagcg gacgagccgg ccgaggtcgg cggggcgatt
     1981 gtaggtcgga atgacgatgg tcagtgccgc cgccatcagc gcgacctgac gaagagcatc
     2041 ctcatgaagc gccggtatag caagaaggca atcgtcgtct tgtccggatc gatgtgctcg
     2101 gacggcgtca tgatcgtgta gcgaggctga cccgtggcgg ctgcggccgg cacgctcgcg
     2161 gacgctgctg ccggctcagg tgccgcggaa ttcagcaagt ccggattgag gtactggcgg
     2221 atacgtggct tgcttccgcc gcgcgcatag tcgagcaggc tcgaatcggc cttcaccttg
     2281 gggtgctgct cgagcgcacg gaaacaatac agcgcgtcgt ggtggatggc ctggtgcgcg
     2341 atcgagacgg ggtcctccac gaggccgcgc tcaagccagt gccgcatgac tcgcccgcga
     2401 tcgtggggga gcgcgatcga gacggcgatc ggcgtgcacg tcgcaatgcc acgctcctca
     2461 aggtacacca tggtcggcac ctgatcacca agcacgccct ggcaaagcac cgcaacatga
     2521 tcgaccttga gtccgagccg agccaagtga gcgaggacgg cgctgcgacc tcccactgcc
     2581 gcagcgtgca cgagcttgac tggatcaacg gcattttcga tgagttcgat cggcaattgg
     2641 cggagcttga ccatgcgccc cgtatggatg agctgctcga gatcgggagc acgctcggct
     2701 gtcaccgtgt ccagcaaact gccctcccct gaattcatgc tcggcgccca acttccaccg
     2761 tactctttcg tttcgaaacg ttcgtcgtgt caaggccact cgctcggccg tcctcatgga
     2821 ttgtcgcggc ggcacgttcg ccatagacgt cgcgcgcggc gcagtttatg tggcgttcga
     2881 gcgcctttgc atttcctcga cagggcagag cgttcgtgcc gcggcttgag atcgatctgg
     2941 tgaaagaact gtgagttgtt ttttcgacag cgatcagaat gcgtcttgaa ggttgaggct
     3001 ggatcgaaaa tgcaaccggg acgtcaggcg ctgatcgatc gacgcccgcc cgccatgcgt
     3061 gcgctggtcg atgccgcgaa gcagggcaat gctcggcgcg tcagcgaatt gctggacgac
     3121 ccgagcgctg caccctacgt gacctgggcg gcctatgcgg cagccgccac cggcggcgtc
     3181 gctgtgacaa aggtcatcgc cgagaaatgc ggcgacgcca ccttgcacga ccgcgagcat
     3241 ctggcattgc ggctcagcgc gcggcgcggc gatctcgaca tgttcaagat gctgctcgat
     3301 acgggcagcc tgctggcggc gaaggatcgc caggccctgc gcctcgcggc gctgcacagg
     3361 cacgacgcca tcatcagcga gggctggcga cgcggctttc cgctctggac catcgaggaa
     3421 ctggtcgccg cggcgcgcgc cgaccgggtc gaccgggtgc gctggatcct gtcggatccg
     3481 aggttcaccg aacagtcttg gcgcgtcgcc ttcgcagccg tcgatgctgg cgcggtcggc
     3541 gtcatccgaa tgctcgcggc gactcgaccg tccgcgctgc atgaccgcaa tcacctagcc
     3601 ctcaggatgg cggcgcgcgc cggccggctc gatgtcttcc aggcgctcct cggctcgggg
     3661 agcgagccgg cagcgcgcgg agggcaggca ctcgcactcg ctcgattgca cgggcatcgg
     3721 cgcatcgcgc gcatctgcga aaatcgcggc gccggcgggt tcgcgccagc aaccgtccgg
     3781 gatctgctcg acgctgcctg ttctggcgat accgcgcgcg tcgacgaact catcgacgat
     3841 ccgatcttcg agcaacacgc gagccgagtt gggatcgcgg ctgcggccgg cggcgccgtc
     3901 gaggtcgtgg agcttctcat ccggcgtctc agcgcgcaag tgctgcacca tcgcgatcac
     3961 ctcgcattgc ggatcgcggc gcggcggggt gacctcgcca tcgtcgacgt gcttctggcc
     4021 aaccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccggc
     4081 atgacgacgt gatcgagcgc ctggtcgagg cgggcgtgcc cattttccgg aaagatgagc
     4141 tttcgaatcc cttcgacgag atcaggagac gggcctctgg acccgttgtc ttcctgcaca
     4201 tcgcgaaaac tgcggggtcg tcgatcggct cgatgctcat gcgcaccttc ccggtcgcag
     4261 aaatcgtgga gtacgacagg gcccaggcga tggccgagcc attgtcgcct tcctactgct
     4321 ggcaccgcca gcctgtggcg aaggcgctcg gtgcactcac tcccgccgag cagtcagcgc
     4381 cccgtctcct cgcaggtcac ttcagctatc gaatacggga ggtgctgccg ccgtcgcggc
     4441 tctatatctc catgatccgc caccccatcg atcgcctcgt ctcgggcctc tactacggct
     4501 cgggtgtcgg gaagcggccg acgaccatcg acgacttcag gctgctctac gattgggact
     4561 acgagatcca gcctaccgac cggtatctgt acatggacag ccaggtgcgg acgctgagcg
     4621 gcgatcccag cctggatccg gacaagttgg ggtcggagaa tgcatttcgc gcagtcacgg
     4681 acgatgtgac ggaccaggtg ctcgcggagg cggccgagaa cttcattctc ctcggcatca
     4741 ctgagcggta cgaggaatcg atgatcgtgc tcgcgcgcca gctcggcgta ccgttgacgc
     4801 tgatcatcga ccggccgcgc aatcgcaccg agcatcggcc tcggctggac gaggttcccg
     4861 ccggggccat cacggcattg accaattaca atcgccaaga cctgcggctc tacgccggag
     4921 ccaaccggct cctcgaccgc atcatcgagg aagaccgaag cggcttcgag cgagaccgcg
     4981 agatcctcgc gcgcctaaag gaaaagcatg ccgcgaagga aggctatccg cgcctcctcg
     5041 cctacgagcg cgcagcgcgg ggcatgccgc cgccgcggct gttctatccc gaggcgccgc
     5101 cgaggcgacc gctcgatgcc gatcgcgcga gtgccgccta tctggacctc atggaagacg
     5161 tactcgtcgg tcgcgtctac caggacccct cgctcgcgcc ctggaatcgg ggccgctaca
     5221 tggcgcaaga tcgccagatc ggcagcgact ggccatcccg cgcgcacagc atgatcggca
     5281 agcagcgcat gcacaacgtg cgaatgctct gcgagcaggc agtgcgcgag ggagttcggg
     5341 gtgacttcct ggaagccggc gtttggcgcg gaggcgcgac gatcatgatg cgggcggtac
     5401 ttcacgctca cgccgatcgc tcgcgcacgg tgtgggtcgc cgacagtttc accggcgtcc
     5461 cgccgccgga cgagcagacc tatcctgccg acacgggcga caagcaccac gccgctgccg
     5521 acgtgctggc gatatcgcaa gcggaagtgg cgagcaactt ccgcagctac gggctgctcg
     5581 atcgccaggt gcgcttcctg cccggtctct tcaaggacac gctcgcccac gcgccgatcg
     5641 agcggctctc cgtcctgcgt ctcgatggag atctctacga gtcgacgatg gacacactcg
     5701 tcgccctcta cgacaaggtg agccccgacg gtttcattat tgtcgacgac tatggcgcgg
     5761 tcccgggctg caaggcggcg atcgaggagt tccgtaagcg ccgcgcgatc gcggcggagc
     5821 tggtcgcaat cgattgggcg ggcgtctatt ggcgcaagcc tcgaactgca atctgatctc
     5881 gcctgtgccg ggcacctgga gcacttcatg gccaccacct cgacaagccc cgcgatcacg
     5941 ttcgtgatcg atgccttgcg gcatgtgcct ctgatccggg tgctggccct caatcacttg
     6001 cgcgagaagt tcatcggcac ctgggccggc atcgtgtggg ccttcataca cccgatcgtg
     6061 ctaatcgcca ttttctgggt ggtatttgcc cagggcttca agctgccgac gagcggcgaa
     6121 aggccgttcc tcctcgtcct catctgcggt ctgctcccct ggatggcctt caacgaggcc
     6181 gtcatggggg ccgcaggatc agtgacctcc aaggcctacc tcgtccgcaa gatcgccttt
     6241 ccgctcgagg tgctgccgct cacgaatgtc gtctcctctt tgatcgtgca cgttccgatg
     6301 ctgctcctgg ggatgatcat tgcggcctgg cacggacgct ttcccggtcc gcatctcgcc
     6361 tggctgcccg tctacatgct ggcgctcgca ctgctcgcaa cgggacttgg catgctgctc
     6421 tcggcgctga gcgtagtctt ccgcgacgtg cagcaggggc tcggcatcgt catgaacatc
     6481 tggttctggg cgacgccgat cgtatgggcg gaggacatgc tgccggcgga gtaccgctgg
     6541 ctttcgcagt ggaacccgat cacctatctc atcgacggct atcgctatgc gctgcttgga
     6601 ccgggcgttc ccgagccgac gacgttcgcg gctgtgctct actggagcgt cacgggcgtc
     6661 gtgatggctg cgggcattct gctcttcaag cgcctgaagc ctaattttgc cgatctgctg
     6721 tgatggcggc cctgagaggg cgaaatgcgt ctgacgatag gtccaatcgc ggtgcttgtg
     6781 gcagcatttc tgatcggcgc cgggacgagc cccgcgcgcg cgcagggcgc tgccttggag
     6841 tataggccgg gtgacgtcga gcgcgcgaag cgcaactcgg gtctcggccg acactgccgc
     6901 taccgcgttt gggtgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggggaag ctcctgccgg
     7081 gcaagcgcga tgccgtgtcg caagccgtga actccgccac cgcaggtgga agttttcccg
     7141 ctcagattgc ctcaagagtt gtcggatcgc atccgcgctg tcagattgcc cgcccactcc
     7201 gacaccgccc ttgacgccgc cgagagtccg cgcttgtttt gttcttgacg atccgcacca
     7261 gcgggcgctc gtcggacctg ctctattcct gctcagttct ctgattgtcg gagtttgcca
     7321 tgtgcgtgtt gtgcggcgac gtttggcatg tgtcgagcga cagctacagg ttcagccctt
     7381 cggccacgac cagtggtgga tcgggatctt catcgatcta cgcgctcgct gagaacggca
     7441 cggaaggtgg gggctcgttc gttggctccg gtgtgccgat gcccggcatc tcgggtgcct
     7501 ccggtcccgg ctcgtccgca ctgctcgccc agacattcag cggcggcgca ttccacttca
     7561 gcggcgaccg caacgtcgac gccgcgttga tcggctcgcg ctggacattc ctcaatcaca
     7621 cctacagctt cccgacgagt ggcaccttct acgtcggcta caccacgggc gacaacacga
     7681 cgggcttctc gacgttcaat gcggcacagc aggcgggcgt cgtttatgcc tacggcctcg
     7741 tctcgcaata cacgccgatg accttcacgc cgatcaccga gacggcgacg acgcacgcga
     7801 cgcaccgctt cgccaacacg acgaacgtcg gttcggccta cggcaacttc ccctccgaca
     7861 acttcgcggc aggcgattcc tggtacaacc tcggccagcc cttctattcc aatccgcagg
     7921 tcggcaactg gggcctcgcc accatcatgc acgagatcgg gcacacgctc ggtctgaagc
     7981 atggccacca ggactacacc gcgctcgatc tgtcgggtta cctgttcgtt gtagggccgc
     8041 gcttcggcag ccgcgcgctc gagtcgacga aggatggcca gccctactcg ctgatgacct
     8101 atagcggcgg cgtcggcatt cccccgacct cgttccaggg tgacggcttc aaccagccgc
     8161 agacgtacat gatgtacgat atcgcggcgc tgcaatacat gtacggcgcc aactacgcga
     8221 gctcgggcca gacgttttcc gggaactctc gatatacctt caatagtttc tcgggcgcga
     8281 tgtacatcaa cggagttccc gaaggcagcc cgcccacctc gaacatcgtt ttccggacgg
     8341 tgtgggacgg tcacggcaac gatacctacg acttagaaaa ttacacgacg ccgatgtcgc
     8401 tgaacctcga ggccggcggc tggctcgtgt tcgataccga ctcgacgggc ggctttctgc
     8461 aacgcgccaa caaccagccg ttgtcgggcg gcccggtctg ggcgcctgga aacgtcgcga
     8521 acgcgcttct ctaccagaac aatgtggcct cgctgatcga aaacgccatc ggtggcactg
     8581 ctgctgacac gatcattggc aaccgcgttt cgaacattct cgatggcggg gatggcaacg
     8641 actcgatcta cggcgccggc ggcgatgact tcctctacgg tcgtgaaggc gccgacacgc
     8701 tctacggcga tgcgagcccg gccggcaact ctggcatctc cttcggcact ggcctcgtca
     8761 caacgcgcgg cacatcgatc gcgacggccg tcaacgtgac ggcgacattc agcctcgcca
     8821 acaacgccga tatcgccaac tcgacgaccg tgccgcacac aacaggtcgc ttcacgacgg
     8881 cggccagcgg caccgtcgtc gcgtcgtatt acgcgatcgt gctcaatgcc ggctcgacga
     8941 tcacgttgga tgtggatcgt acgagcggcg ctctcgactc atggatcagg ttctacaaca
     9001 gcgccggcac cgtgctcgca cagaacgacg atgccggcgg cgacgcggga tccaccggcg
     9061 tgacgatcga ttccgcgttg acctattcga tcactgcaac agggacctac tatttctcga
     9121 tcggtcagta ttcgggaggc gcctacctcg acacgctccc gaacagcgtg acgtacgacc
     9181 tgaacgtgtc ggtcgaggca ccggccgcga tcggcaacga cggcgcggcc ggcaacgaca
     9241 ttctgaacgg tggtccgggt ggcgaccagc tcttcggcgg cacgggcttc gactacgcga
     9301 gctacgcgac ggcggtggcg ggcgtggcgg cgttcctgac ggtgccgggc tccaatacgg
     9361 gcgaggctgc cggcgacagc tacaacagca tcgagggcc
//
LOCUS       DAORNH010000177         9359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5440_length_9359_cov_2.776548, whole genome shotgun sequence.
ACCESSION   DAORNH010000177 DAORNH010000000
VERSION     DAORNH010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5440_length_9359_cov_2.776548"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PK264_16475"
     CDS             <1..712
                     /locus_tag="PK264_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRD77503.1"
                     /translation="VVVNGENAAGGFGITEQILHDLLDAGADVVTLGNHAFDQKDTLV
                     FISREPRLIRPINFPPGTPGKGAGMFQTRSGANVLVMNVMGRIFMGEYDCPFRAVDRE
                     LTACRLGEGADAIIIDVHAEATSEKQALGFFADGKASLVVGTHTHVPTSDARILPGGT
                     AYMSDVGMTGDYNSVIGMEADEPLHRFLTKIPRGRFEPASGPATLCAVLVETDDKSGR
                     ATSVAPIRIGGALSQALT"
     gene            792..1559
                     /locus_tag="PK264_16480"
     CDS             792..1559
                     /locus_tag="PK264_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77504.1"
                     /translation="MRILFIPFFLSFILPTATLIAQPKSVPLPAVERSADVSSCGSTC
                     TPRLHLAQVPTNSDAGKAEPGKAPLPDPEAAAKSAPPGGTVDGTVRNMTRAPVQIGDL
                     FSKDDLIRLQELAAKYGMGVPAFEFVIPGPEIAPVRRRLVGAWINEAGNNGKGRRLLV
                     IITSATADGVKGFLSIGASDESSFVKGPATFFPFAAPLDGATVRFKNPKGTAEYSLTL
                     AADNQLVYSYVNIKGQTTGRRLRPIWMLVEAERKTRP"
     gene            1678..2184
                     /locus_tag="PK264_16485"
     CDS             1678..2184
                     /locus_tag="PK264_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77505.1"
                     /translation="MSKLKMGALLAALALGGCSSGGITTGSILGVSAAKAPAAEDPST
                     RAIHVAATSAKALRCGYNFDTERLKASYLSWEGRSGQPPEQMGKIAQAYDFSYQSVTR
                     SIPNAEAYCTDAVAREIKPDLTRYLAGDFTAPASAVVAAAPAAVKKVEPMDTARVFDP
                     TGVRGPRP"
     gene            2670..3806
                     /locus_tag="PK264_16490"
     CDS             2670..3806
                     /locus_tag="PK264_16490"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRD77506.1"
                     /translation="MAGNTALGVLATALIAGPYLWNWLSLPRLRLDSFPMPTAFDSIP
                     ARLILGFFTLCVLVFAIAAGVKSWEVRKAARWSIANGRIVRSEQAIELVRRGHGEMPV
                     NTRVAKIAYEFEAGGRTLRGTRFTLGEITPESEVAGILARYPLNAPVDVYYDPENPAE
                     CTLERDPPEGILKGCLILLAYGIVGVLGLMWLVSGGARQLADMLSLSDRLPGAFLPVV
                     GMAGFAGLFLLGFFLAIEMRQRRARQWPKTTGEVVLSTIHEYYQGSYRTETPISVARS
                     KRRGYMPIVEYTYKVMGRPYSSRHIQIESQTAGSRSYAESVIARYGKGQVVTVHYNPE
                     QPSQAALELGGRLHYVLLVLGALLMLFALNAAGILGDSIFVPRR"
     gene            complement(3831..>4664)
                     /gene="ychF"
                     /locus_tag="PK264_16495"
     CDS             complement(3831..>4664)
                     /gene="ychF"
                     /locus_tag="PK264_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRD77507.1"
                     /translation="NQFLAHIREVDAVAYVVRCFEDDDITHVENRIDPIADAETVETE
                     LMLADLDSLEKRRSTIEKKAKTGDKDAKAQVVLIDKALALLREGKPARLVALTPEEVA
                     PFRLLQLLTAKPVLYVCNVDEGSAKAGNAHSRRVEERAKAEGAGCVAISAKIEAEFAG
                     LAPEEREAFLTDLGLEEPGLNRLIREGYNLLGLITYFTVGPKEARAWTVTAGTKAPQA
                     AGVIHTDFEKGFIRAETIAYTDFVTLGGEVGAKDAGKMRLEGKEYVVRDGDVMHFRFA
                     N"
     assembly_gap    4665..4674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4675..4932)
                     /locus_tag="PK264_16500"
     CDS             complement(<4675..4932)
                     /locus_tag="PK264_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRD77508.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAEAANYPFCTIEPNVGE
                     VAVPDERLERLAGIAKSAQRLPTRITFVDIAGLVRGASKGEG"
     gene            complement(5101..6225)
                     /locus_tag="PK264_16505"
     CDS             complement(5101..6225)
                     /locus_tag="PK264_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRD77509.1"
                     /translation="MNFTEHAGKEVLKAHGIPVPEGLLARTPDEARAAAARIGPCVVK
                     AQVPTGKRGKAGGIKLAATPDAAAEAARNILAMSIADHPVEAVLIDPQVDIAREMYAA
                     VLNDPDSKSPLLLFSPEGGMDIEELADAHPDALLRFPVDIRHGPDQELLAAAVRRYDP
                     PAVHQITEILLALYRAYAATDAELVEINPLAITRAGKFVALDCKLAIDDSAIPRRHEL
                     STTGVPDRLTALETRARELHLKYIELDGEVGVLANGAGLTMTTMDAIRHYGGRPANFM
                     EIGGDSYTKAKPALEIVLANPRVKCLLVNFCGAFARTDVMAEGVVGAWLDLKPKIPII
                     FTIHGTGEDEAIALVKSRLGIEPHDLMDDAVKAAVAAASA"
     gene            6368..6796
                     /locus_tag="PK264_16510"
     CDS             6368..6796
                     /locus_tag="PK264_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77510.1"
                     /translation="MHRSQGLGRLFYRLAVLCLAAGTVVLTSDIHAEPVKGAMRVAAG
                     KTKLVFVVAGFDTECRSTTLPVVSVDAPAAKGKVVLKAGETTTVQYSLSGRCIGAKVQ
                     GVAIYYVAADDAVGADAFSISARMPTGEIATRMFNLHIVD"
     gene            complement(6806..7894)
                     /locus_tag="PK264_16515"
     CDS             complement(6806..7894)
                     /locus_tag="PK264_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD77511.1"
                     /translation="MSTLPPPLEFGWFLPTNGDTTSYARPVEVPQSIAMFERVTAAAE
                     AAGFSYMLVPVQTVCWEAWIAGAMMAARSSRIKMLIAARPGYINPVHLAKMIATFDQL
                     SGGRISINLIAGQSETEIASEGIRYSKEDRYALMEEEVRILKSLWTSKGPLDFEGRFH
                     SLKGAQVRPRLVQQPSPKFYLGGGSREAGEMSARHSDVHLFWGDYPERIARQMAEIRA
                     MAATYGRAGTIGFGMRLQIICREREADAWAAARQLVAGVSAKHVEMTATHFATSVANQ
                     RMMELARAETDMLGPHLWSGITRVRPGAGVAIVGNPEQCAATLQTFIDLGCHSFCLSG
                     WLHDEEAERFGRLVLPLVRKANPTRAFT"
     gene            complement(8405..8764)
                     /locus_tag="PK264_16520"
     CDS             complement(8405..8764)
                     /locus_tag="PK264_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77512.1"
                     /translation="MLRSPVLIFVVLATALVVGRIVYLINDGPGGVRLERGKAASVAK
                     NPRTEHRQGKLWKAFRGERSFDGIKAPCGEGSSGSLKGKRYFMVDAGDNEIHALHGVF
                     APLPVREIYDYIDELSR"
BASE COUNT         1584 a         2930 c         3168 g         1667 t
ORIGIN      
        1 cgtcgtcgtc aacggcgaga acgcggcagg cggcttcggc atcaccgagc agatcctgca
       61 cgacctgctc gatgcgggcg ccgacgtggt gacactcggc aatcacgcgt tcgaccagaa
      121 ggatacgctc gtcttcatct cgcgcgagcc gcggctcatc cgcccgatca actttccgcc
      181 ggggacgccg gggaagggcg ccggcatgtt ccagacgcgc tcgggcgcga acgtgctcgt
      241 catgaacgtg atggggcgca tcttcatggg cgagtacgac tgcccgttcc gcgccgtcga
      301 ccgcgagctc accgcctgtc ggcttggcga aggggcggac gcgatcatca tcgacgtgca
      361 tgccgaggcg accagcgaga agcaggcgct cggcttcttc gcggacggca aggcgagtct
      421 cgtcgtcggc acgcatacgc acgtgccgac cagcgacgcg cgcattctgc cgggtggcac
      481 ggcctacatg tcggacgtcg gcatgaccgg ggactacaac tcagtgatcg gcatggaggc
      541 ggacgagccg ctgcatcgct tcctcacgaa gatcccgcgc ggccgcttcg agcccgcgag
      601 cgggccggcg acgctctgcg ccgtgctcgt cgagacggac gacaagagcg ggcgtgcgac
      661 gagcgtggcg ccgataagga tcggcggggc gctttcgcaa gcacttacct gatcgagatc
      721 atggctattg gatggcgttc gaagtaaggt tggaccgatc gccagcattg cttgctcgat
      781 ggagtgaccc gatgcgcatt ctatttatcc cgttcttcct gtcattcatc cttccgaccg
      841 ctacgctcat cgctcagccc aaatctgtgc cgttaccggc ggtagagcgg tcggcagacg
      901 tcagttcctg cggctcgacc tgcacgccac gacttcacct cgcgcaggtt ccgacgaaca
      961 gcgacgcggg caaggccgaa ccgggcaagg cgccgctgcc cgaccccgag gcggcggcga
     1021 agtcggcgcc gcccggtggc acggttgatg gtaccgtgcg caacatgacg cgggcgccgg
     1081 tgcagatcgg cgacttgttc agcaaggacg acctgatccg cctccaagag ctcgcggcca
     1141 agtatggcat gggcgtgccg gcgttcgaat tcgtcattcc gggaccggaa atcgcaccgg
     1201 tccgacggcg cctcgtcggt gcctggatca acgaggccgg caacaacggc aaaggccgcc
     1261 ggctcctcgt cattatcacc tcggcgacgg cggacggcgt caagggcttt ctcagcattg
     1321 gggcctcgga cgaatcgagc ttcgtcaagg ggccggcgac cttcttcccg ttcgcggctc
     1381 ccctcgatgg cgcgacggtg cgtttcaaga atccgaaagg gacggccgag tattcgctga
     1441 cgctcgccgc cgacaaccag ctcgtctact cctatgtgaa catcaagggg cagacgacgg
     1501 ggaggcggct tcggcccatc tggatgctgg tcgaggccga aaggaaaacc cggccgtagc
     1561 ggcgctgagc ggcctcgcgt ttgccgtagt tgggggttct ctggtgacgt gcccgggctg
     1621 caattgctat ccggctacgg ctgacgcaga ctatacgcgt cgaatcgcga ggcgatcatg
     1681 tcgaagctga agatgggtgc cctacttgcc gctttggctc ttggcggctg ctcgagcggc
     1741 ggcatcacca cgggaagcat cctcggtgtc agcgcggcca aggcgccggc ggccgaagat
     1801 ccctcgacac gcgccattca tgtggcggcg accagcgcca aggcgctccg ctgcggctac
     1861 aacttcgata ccgagcggct gaaggcgagc tacctttcct gggaggggag gtcgggacag
     1921 ccgcctgagc agatgggcaa gatcgcccag gcctacgact tctcctacca gagcgtcacc
     1981 cgcagtatcc cgaatgcaga ggcctattgc acggacgcgg tggcgcgcga gatcaaaccg
     2041 gatctaacgc gctatctcgc aggtgatttc acggcgcccg catcagcggt ggtagcggcg
     2101 gcgccggcag cggtgaagaa ggtcgagccc atggatacgg cccgcgtgtt cgacccgacg
     2161 ggagtacgcg gcccgcggcc ttgaggggct gtgcgcgttg aagctgcgcg cccgactgga
     2221 cgcagtaagc actcaatggg aatgcacggc tcgcttggtg ccggtgtgat atagctgtgg
     2281 gcctgcgtac gacccaggcc aacgcggaag agatgcgtct cgagatcaat ggaagagtga
     2341 tcgatgcctc gaaggaggca gacgtctcga tccaaattcc ggatcgggcg ccgggcgcgg
     2401 cttggcgcct caagctcaca ggcgacaagg ctctgtcgat cgaggcggca gggcgggccg
     2461 atggtcgctt cgacgtgtcc catgccgcag gtcgcaaggc ctacgatgcg tcggctccga
     2521 tcgatggtca cgggctgaga gcgctcttgc ggcgttgtct cgagggcgac aagacctggc
     2581 gaaatcagct gcaatggcgg cgtcgccaag tcgttgatca gcggcgcccg tccgctcctc
     2641 cagttcggac cgacagcagg tggaagacag tggccgggaa cactgcgctc ggcgtgctcg
     2701 caaccgcctt gattgccggt ccttacctct ggaattggct ttcgttgccg cgcctccggt
     2761 tggacagctt tccgatgccg acggcgttcg attcgattcc ggcacgcctg attctgggct
     2821 tcttcacact gtgcgtgctc gtgttcgcga ttgccgccgg ggtgaagtcg tgggaggtgc
     2881 gcaaggcggc ccgttggtcg atcgccaacg ggcggatcgt gcgttctgag caggcgatcg
     2941 agctcgtgcg acggggccat ggcgagatgc cggtcaacac gcgggtcgcc aagatcgcct
     3001 acgagttcga ggccggcggc cgcaccctgc gcgggacccg cttcacgctc ggcgagatta
     3061 cgcccgagag cgaggtggcc ggcatccttg cccgctatcc tctgaacgcg cctgtcgacg
     3121 tctactacga tcccgagaac ccggccgagt gcacgctcga gcgggatcca cctgagggca
     3181 ttctcaaagg ttgtctgatc ctgctcgctt acggcattgt cggcgtactg ggcctcatgt
     3241 ggctcgtctc gggcggggcc cggcagctcg ccgacatgct gtcgttgtcg gatcggcttc
     3301 caggagcctt cctgccggtc gtcggcatgg caggtttcgc tggcctcttt ctgctcggtt
     3361 tctttctggc aatcgagatg cgtcagaggc gcgcgcggca gtggccgaaa acgacgggcg
     3421 aggtcgtcct cagcacgatc cacgagtact accaaggctc gtatcggacc gagacaccga
     3481 tctccgtggc tcgcagcaag cggcgcggct acatgccgat cgtcgagtac acctacaagg
     3541 tgatggggcg gccatacagc agccgtcaca tccagatcga gagccagacg gcgggctcgc
     3601 gcagctatgc agagagcgtg attgcacgct acggaaaagg gcaggtcgtg actgtccact
     3661 acaacccgga gcagccgtcg caggcggcac tcgaactcgg cggcaggctc cattacgtcc
     3721 tgctggtcct cggcgcactc ctcatgctgt tcgcactaaa tgcagcaggg atcctcggag
     3781 acagcatctt cgtgccgagg cggtgacatc gtgggaggcg ggagtccggc tcagttcgcg
     3841 aagcggaagt gcatgacgtc gccgtcgcgg acgacgtact ccttgccttc gaggcgcatc
     3901 ttgccggcgt ccttggcgcc gacctcgccg ccgagggtga cgaagtcggt gtaggcgatc
     3961 gtctcggcgc ggatgaagcc cttctcgaag tcggtgtgga tgacgcccgc ggcttgcggc
     4021 gccttggttc cggcggtcac cgtccaggcg cgcgcctcct tcggtccgac cgtgaaatag
     4081 gtgatgaggc cgagcaggtt gtagccttcg cggatgaggc ggttgaggcc cggctcctcg
     4141 aggccgaggt cagtgaggaa ggcctcgcgc tcttcaggtg cgagcccggc gaactcggct
     4201 tcgatcttcg ccgagatcgc gacgcagccg gcgccctcgg ccttggcgcg ctcctcgacg
     4261 cggcgggaat gcgcgtttcc ggccttggcc gagccttcat cgacgttgca gacgtagagg
     4321 acgggcttcg ccgtcagcaa ttgcagcagg cgaaagggcg cgacctcctc gggcgtgagc
     4381 gctacgaggc gggcgggctt gccctcgcgg agcaacgcca gcgccttgtc gatcagtacc
     4441 acttgcgcct ttgcgtcctt atcgccggtc ttcgccttct tctcgatcgt ggagcggcgc
     4501 ttctccaggc tgtcgaggtc ggcgagcata agctcggtct cgaccgtctc ggcatccgcg
     4561 atcgggtcga tgcggttctc gacgtgtgtg atgtcgtcgt cctcgaagca gcgcacgacg
     4621 taggcgaccg cgtcgacctc gcggatgtgg gcgaggaact ggttnnnnnn nnnngccctc
     4681 gcccttcgag gcgccgcgga cgagcccggc gatgtcgacg aaggtgatgc gggtcggcag
     4741 gcgctgggcc gacttggcaa tgccggcgag cctttcgagg cgctcgtcgg ggacggcgac
     4801 ctcgccgacg ttcggctcaa tcgtgcagaa cggatagttg gcggcctccg ccgccgcggt
     4861 ctgcgtcagc gcgttgaaga gcgtggactt gccgacattc ggcagtccga cgatgccgca
     4921 tttgaagccc atgatcggtc cctggcaatg gcgcaagcgc gccgcctcgc gttggccacg
     4981 tgcctaggcg ttgggaggcc aaatggaaag cctgttattg ccgcaggcgt gcgggaaagt
     5041 tcggggcgct gccccgaacc ccgccagagg cctcggcctt tggaaatcat ttgttttgcg
     5101 tcaggcactc gccgccgcga cggcggcctt gacggcgtcg tccatgaggt cgtgcggctc
     5161 gatgccgaga cgcgatttga cgagggcgat cgcctcgtcc tcgcccgtgc cgtggatggt
     5221 gaagatgatg gggatcttcg gcttcaagtc gagccaggcg ccgacgacgc cctccgccat
     5281 cacgtcggtc ctcgcgaatg cgccgcagaa gttgacgagg aggcacttca cgcgcgggtt
     5341 ggcgagcacg atctcgagcg ccggcttcgc cttggtgtag ctgtcgccac cgatctccat
     5401 gaagttcgcg ggtcggccgc cgtagtggcg gatcgcgtcc atggtggtca tggtgaggcc
     5461 ggctccgttg gcgagcacgc cgacctcgcc gtcgagctcg atgtatttga gatgcaactc
     5521 acgcgcgcgc gtctcgagtg cggtgaggcg gtcggggaca ccggtcgtcg aaagctcgtg
     5581 acggcgcggg atggcgctgt cgtcgatcgc gagcttgcag tcgagggcga cgaacttgcc
     5641 ggcgcgcgtg atggcgagcg gattgatctc gaccagttcg gcgtcggtcg cggcataggc
     5701 gcggtagagc gcgagcagga tctcggtgat ctggtggacg gcgggcggat cgtagcggcg
     5761 gacggcggcg gcgagcagct cctgatcggg gccatgccgg atgtcgacgg ggaagcgcag
     5821 gagcgcgtcc gggtgcgcgt cggcgagctc ctcgatgtcc atgccgcctt cgggcgagaa
     5881 gaggaggagg ggagacttgc tgtcgggatc attgaggacg gctgcataca tctcgcgggc
     5941 gatgtcgacc tgcggatcga tcagcacggc ctcgaccgga tgatctgcga tgctcattgc
     6001 gaggatgttg cgagcggcct cggcggcagc gtcgggcgtc gctgcgagct tgatgccgcc
     6061 ggccttgccg cgcttgcctg tcggtacctg ggccttcacg acgcaggggc cgattcttgc
     6121 agccgcggca cgtgcctcat cgggcgtccg agccaagagg ccctcgggca caggaatgcc
     6181 gtgggccttc agcacctctt tgccggcgtg ctcagtgaag ttcatgagga tggccgatct
     6241 gctggggaca tgcctgccgt gagcctacca gatgaggaag gtcgctcgga agacccgatc
     6301 ggccgagcgg caaggcggtg gcccgtggta aattggatca gtgagcgtcg attcgcggag
     6361 agacggtatg caccgctccc agggcctcgg acgactattt taccgcctcg ccgtgctttg
     6421 cctcgccgcc ggtacggtcg tgctcacgag tgacatccat gccgagcccg tcaagggcgc
     6481 gatgcgtgtg gccgcgggca agacgaagct ggtgttcgtg gtagctggct tcgacaccga
     6541 gtgccgttcg accacgttgc ccgtggtctc ggtggacgcg ccggcggcca aaggcaaggt
     6601 cgtcctgaag gccggcgaga cgacgacggt gcaatactcg ctctcgggcc gctgcatcgg
     6661 cgcgaaggtg caaggcgtcg cgatctacta cgtcgcggcg gacgatgcgg tcggggctga
     6721 cgccttctcg atcagcgcgc gcatgccgac gggcgagatc gcgacgcgca tgttcaacct
     6781 gcacatcgtc gactgaagtc gcccgtcagg tgaaggcgcg ggtcgggttg gccttgcgca
     6841 cgagcggcag cacgaggcgg ccgaagcgtt cggcttcctc gtcgtggagc catcccgaca
     6901 ggcagaacga gtgacagccg aggtcgatga aggtctgcag tgtggccgcg cactgctcgg
     6961 gattgccgac gatcgcaacg ccggcccccg ggcgaacccg tgtgatgcca ctccacaagt
     7021 gggggccaag catgtcggtc tccgcgcggg cgagctccat catgcgctgg ttggcgacgg
     7081 aggtcgcgaa gtgcgtggcc gtcatctcga cgtgctttgc cgagacaccg gcgacaagct
     7141 gacgggccgc agcccacgcg tccgcctcgc gctcccggca gatgatctgc agccgcatgc
     7201 cgaagccgat cgtaccggca cggccatacg tcgccgccat cgcccggatc tccgccatct
     7261 gtcgagcgat gcgttcggga tagtcgcccc agaagaggtg gacgtcggaa tggcgcgcgg
     7321 acatctctcc cgcctcgcgc gaccctccgc caaggtagaa tttcggcgag ggctgctgca
     7381 cgaggcgcgg gcggacctgc gcgcctttga gggagtggaa gcgcccttcg aaatcgagcg
     7441 ggcccttgct ggtccacagg gacttgagga ttcggacctc ctcctccatc aatgcgtagc
     7501 ggtcctcctt cgaatagcgg atgccttctg aggcgatctc cgtctcgctt tgccccgcga
     7561 tgaggttgat cgagatgcgc ccgcccgaga gctggtcgaa ggtcgcgatc atcttggcga
     7621 gatggaccgg attgatgtag ccgggccgcg ccgcgatcag catcttgatg cgcgaggaac
     7681 gcgcggccat catcgcgccc gcgatccagg cctcccagca caccgtctga acgggaacca
     7741 gcatgtagct gaagccggcc gcctccgccg ccgccgtgac gcgctcgaac atggcgatgg
     7801 attgcggcac ctcgaccgga cgcgcgtagc tcgtcgtgtc gccgttggtc ggcaggaacc
     7861 agccgaattc gaggggtggg ggcaaagtgc tcatggcgcc aacatgtcga agtgccgtct
     7921 cggtctgcca gattggctga aggacccgag cgtatgcaag aggaggagtt cgcgcgctgg
     7981 gccggctcgc ttcgcgaacg gggctcccgc cccgtgcccc ggccggagga gatcctccgg
     8041 acctcccctt tttgcgacga gtagggcagc gcttcgccag atcaagccaa aaaggcgcgg
     8101 gtccggggcg cgcagccccg ggcgggtccg ggcccgcggc ccggttgcgc agcaagtcga
     8161 tgtcctcgga cgcctacgta tcagtcagct cacgccacat cgtgccagtg ggccgcgcgc
     8221 cggtccgttc gaactcgggg catcacggat gcagcgcggc cgaagccgat ggagacctcg
     8281 gtcggcccgt cttggtctcc ggcatcccta gctgcgtcat tcgcataata tatattatgg
     8341 aaaatactgg cttctgatag agttattaac aatagtttgc ggtactggct gttctcttca
     8401 atcgctaccg gctcagctcg tcgatatagt cgtagatctc gcgcacgggg agcggtgcga
     8461 acacgccatg cagcgcgtgg atctcgttgt cacccgcatc caccatgaag tagcgcttgc
     8521 ccttgagcga accgctgctc ccctccccgc acggcgcctt gataccgtcg aaggagcgtt
     8581 caccgcggaa cgccttccag agcttgcctt ggcgatgctc ggtgcgggga ttcttagcga
     8641 cgctcgcagc cttgccgcgc tcgaggcgga caccgccagg cccgtcgttg atgagataga
     8701 cgatgcgacc gactacaagc gccgttgcca gaacgacgaa tatcaggacg gggctccgca
     8761 gcatcaaaga acggagaccc catcgaggaa aagtgactga gccgatcgtc aggcgggcag
     8821 ctctgtggaa tagctagccc gccatgccga gttcaagtct cggtgctcac ctgcacttgt
     8881 aaccccattc cacacatcgt ccatagctat tgggaccaaa aacgcgacgc ttgcataccc
     8941 tcacagccgt ccggccgaac ccgcatccat acttcgggac attctgcacg tgctcgagcg
     9001 gtggtggcac aatggccgga gccgcggtcc tgggcatcgc cgatgcgatg ccggcgaacg
     9061 caccgacgct gcaggcagcc aagaaagcga tcctgaaact tttcatgaaa ggctcccgaa
     9121 taccaagtgc tctgatgaca aaagcgaagc gatgtaccgg gatgtcgcca agggtgacgt
     9181 ttcacacgtt gaaatgacgc gagagcatca gccgcttgaa agtcagtgct tgagaaagtc
     9241 caacgtccaa acatcccaca ctttttttac tatggtcatt acacggctgt ccagtacgga
     9301 caggtgagct cactgtttcg cggacgcgcc ttggcttgct cgccccaagg tgacgccga
//
LOCUS       DAORNH010000178         9359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5441_length_9359_cov_2.440026, whole genome shotgun sequence.
ACCESSION   DAORNH010000178 DAORNH010000000
VERSION     DAORNH010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5441_length_9359_cov_2.440026"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PK264_16525"
     CDS             <1..884
                     /locus_tag="PK264_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77513.1"
                     /translation="LVMVFVMALGLPLKDPGENALPTPKTPEYVKLAALAPANDPPLQ
                     PFRRPPPDPKPEEKKPGFVAPPPPGVEGDLARPDRVSRCFSVEGDKLTWYMDTRIGET
                     ARVPPNAPFPRNPLPHCGTARIGAGVAPSGPDRDGGAEHGLQFPDYRWELTVAGQRCL
                     VEICGALLDKVAAERDRLKYVLIEGHTSSGWRDPASCRPMARAATAADKKLAADACNR
                     ELSVKRAMTAFEFCRGYNLTERQLSEEEFAARFRTDGRGSLDVRMDRDTGREDPIASR
                     RVEFVFVRTDAGRHAAK"
     gene            1022..1681
                     /locus_tag="PK264_16530"
     CDS             1022..1681
                     /locus_tag="PK264_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD77514.1"
                     /translation="MIKIYGVYRSRATRNIWLANELGLKFEHIPVIQVYRLPDPHAPG
                     AALHTQSPAFLKVNPNGRIPAMVDGDLVLNESLAINLYLAKKAGGPLAPANLAEDARM
                     TAWTLWSVTEVEPHSIQILYHLVAKPVAERDPKVAKAAIEALRAPLKVLDQALAAGGG
                     FVVGRRFTVADINVAEVVRYALPATELLAEAPNVKAWIAACHARPAFKDMMAKRELEP
                     A"
     gene            1681..2796
                     /locus_tag="PK264_16535"
     CDS             1681..2796
                     /locus_tag="PK264_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HRD77515.1"
                     /translation="MNAKPYHPIFSPEPRHHTEAEFSREEVMLAHHNNAFPLEALAHD
                     VTPAGLHYVLTHFDIPYVPDAAAWRLAVLGAVPNAGSFSVADIRAMPQRTLRVTLECA
                     GNGRIHIKPRWRSMPWSHEAVSTAEWTGTPLKNLLAAVGVKDKFASGVVEVSFLGVDR
                     GFDKGVEHNFGRSLKPAMALSDDVLVVHAMNGQPLLPQHGFPLRLIVPGWYGMASVKW
                     LDRIEVLTRPFDGFQQVGTYHFRDRSGGAPVPVTTMRVKSLIKPPGIPDWYSRARLLD
                     RGRVVLEGRAWAGGGRAIKSVLVAIDGVLRAAFVHPKPGPFAWQKWTCEWDATPGEHI
                     LACRAIDDAGAEQPLDAPYDAGGFGNNGAHMMTVTVR"
     gene            2814..>3194
                     /locus_tag="PK264_16540"
     CDS             2814..>3194
                     /locus_tag="PK264_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD77516.1"
                     /translation="MADTIEVHVWRGEETGRYQVFEVPLRESQTVLDLVTWVQRHTDP
                     TLAYRFACRVGMCGSCAMTVNGRPRWTCRTHVSKVIENGRIEIGPLANLPVIRDLVTD
                     MTRFFEKWQAAKGAFEPSKSRHDAL"
     assembly_gap    3195..3204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3205..3542
                     /locus_tag="PK264_16545"
     CDS             <3205..3542
                     /locus_tag="PK264_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRD77517.1"
                     /translation="PISPQTKERIAADEAIECINCGVCYAACDTVRWNEDYLGPAALN
                     RAWTLVNDVRDAGNADRLWAVSRDGGCHNCHSHQSCEQHCPKALNPTSAIAGLKRRTM
                     QAYIRGELK"
     gene            3539..3868
                     /locus_tag="PK264_16550"
     CDS             3539..3868
                     /locus_tag="PK264_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HRD77518.1"
                     /translation="MNVRLYVLQRLTATAMVPLIAIHLATMIYAIRGGLTATEILSRT
                     RGSVGWALFYGLFIMLAAVHGAIGVRGVLREWSRLDARALDLVMWGVGLILVLLGLRA
                     VAAVVLP"
     gene            3865..4221
                     /locus_tag="PK264_16555"
     CDS             3865..4221
                     /locus_tag="PK264_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b subunit"
                     /protein_id="HRD77519.1"
                     /translation="MRRPSNSAYRRDTLWLTAMVHRVSGVLLACFLPLHFLALGLAIN
                     GAARLDGFLKWTDNRWVKAAEVGLVLLLAVHLLGGLRILVIENMPWRDGQKRMALGAV
                     AIATVVAGAFLIGALT"
     gene            4318..5139
                     /locus_tag="PK264_16560"
     CDS             4318..5139
                     /locus_tag="PK264_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD77520.1"
                     /translation="MAYAINVMAGAAVLYGGIVLAAWLGQRKLMYHPNTTRTAPAAVG
                     LVGVEERLVETDDGARIVTWQVKAEPGRPTILYFHGNAGDLADRAERIRQFSAAGWGA
                     VMMAYRGYAGSTGSPSEAANVADAKRVFDRLVAEGTRAESIIAYGESLGSGVAVQLAA
                     SRPVRALVLDAPYTSIVDVAASVYPYLPVRPLLKDRYESSRHLGVIKVPVLILHGERD
                     QVIPIAMGRAMYALANEPKRMVEFPAGNHSDLFSHGALEAIRRWLGEVGAAEPAR"
     gene            complement(5152..5520)
                     /locus_tag="PK264_16565"
     CDS             complement(5152..5520)
                     /locus_tag="PK264_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77521.1"
                     /translation="MLTANFVLWMIPGPLLSGIVFGALFAVTYMPFNRWLAKKVGGPY
                     KAIAAVPMTRAQTISLGLSTGAFFGLFMFVKSVAEAAKISDVFWFLVMGWTLLAYLAS
                     IPIMRLRAEWEKARAAERQA"
     gene            5716..6099
                     /locus_tag="PK264_16570"
                     /pseudo
     CDS             5716..6099
                     /locus_tag="PK264_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            6220..7431
                     /locus_tag="PK264_16575"
     CDS             6220..7431
                     /locus_tag="PK264_16575"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRD77522.1"
                     /translation="MKTRTSALALAGALTAFALGAIAYAQEKRAADAGGLVKKEVFTM
                     ETYKTVSGKEVKKLKAGWQAMGTLNADKSNAILVTHFFSGTSHFAGKYKAEDKAAGYW
                     DAIIGPGKAVDTNKYYVLSMDTLVNLNAKDPMVTTTGPASIDPATGKPYAMKFPIVTI
                     RDFVNVQKALLDQLGIKKLHAVMGASMGALQAYEWAASYPDMVGRVIPVIGAGESDGY
                     LIAWLDMWAAPIKVDANWKNGDYYGGPEPTAGLAAALKIVTLHAQHWAWTNSAPFGRK
                     WAAADKNPADCMLDCRYAIETVLDGAGRARAAISDANHFLYLVKANQLFAAGGNIEEG
                     LKKINAPVLLITTDDDLVFHVDGVKRTVDLIKADGTPVEHVKIKGGRGHLDGVLHIGQ
                     AAAQLTAFLAK"
     gene            7970..8365
                     /locus_tag="PK264_16580"
     CDS             7970..8365
                     /locus_tag="PK264_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77523.1"
                     /translation="MILPQKFRSYTMAILGLAGTGALAVSSPLWTGSPAIAQVTFGSL
                     TCDYERKVRSTDSSTATTITFINKAKTYRGLNWIDFKGQQKSFGGLNPGERRTISTYR
                     THPWVVTTGPGDCIKIYLPTAFPATVTLR"
BASE COUNT         1609 a         3166 c         3002 g         1572 t
ORIGIN      
        1 tcctcgtcat ggtttttgtg atggcgctcg gcctgccact caaggacccc ggcgaaaatg
       61 cgctgccgac gccgaagact cccgagtacg tgaagctcgc cgcactcgcg ccggccaatg
      121 acccgccgtt gcaacctttc cgccggcccc cgcccgaccc gaagcccgag gagaagaagc
      181 cgggcttcgt cgcgccgccg ccgcccggcg tcgaaggcga tctcgcgcgg ccggatcgcg
      241 tcagccgctg cttttccgtc gaaggcgaca agctcacctg gtatatggac acgcgcatcg
      301 gcgagaccgc acgcgttccg cccaatgccc cttttcctcg caatcccctg cctcactgcg
      361 gcaccgcgcg gatcggcgcg ggcgttgcgc cttccgggcc agatcgcgac ggcggtgccg
      421 agcatggcct ccagtttccc gactaccgct gggagctgac cgtcgccggc cagcgctgcc
      481 tcgtcgaaat ctgcggggca ctcctcgaca aggtcgcggc cgagcgtgac cgcctcaagt
      541 acgtgttgat cgaggggcat acctcgtccg gttggcgcga tcccgcgagt tgccggccga
      601 tggcgcgagc ggcaacggcc gccgacaaga agctcgccgc cgacgcctgc aaccgcgagc
      661 tttcggtgaa gcgcgccatg accgcattcg agttctgccg cggctacaac ctcacagagc
      721 gccagctcag cgaagaggag ttcgccgctc gcttccgcac cgacggccgc ggctcgctcg
      781 atgtgcgcat ggaccgggac accggccgag aggatccgat cgcctcgcgc cgcgtcgaat
      841 tcgtgttcgt gcgcactgat gccggccgac acgcagcgaa gtgatcgaag cctttggccg
      901 cgcgcgtcga ggcaggagtg tgatgatccc cacgtgacgt gatgctcgcc gatttcgcca
      961 ttgtctcgag ccggcagcgc acatacgatc gctcggcacg cttcacaagg cccgccccga
     1021 catgatcaaa atctatggcg tctatcgctc gcgcgcgacg cgcaacatct ggctcgccaa
     1081 cgagctcgga ctgaagttcg agcacatccc cgtgatccag gtctaccggc tacccgatcc
     1141 gcacgcgccg ggggcggccc tccacacaca atcgcccgca ttcctgaagg tcaatccgaa
     1201 cggccgcatc ccggcgatgg tggatggcga tctcgtgctg aacgaatcgc ttgcgatcaa
     1261 cctctacctc gcgaagaagg ccggcgggcc gcttgcaccg gctaatctcg ccgaggacgc
     1321 gcgcatgact gcctggacgc tctggtcggt gaccgaggtc gagccgcatt cgattcagat
     1381 cctctatcac ctggtggcaa agcccgtggc cgagcgcgat ccgaaggtcg ccaaggccgc
     1441 gatcgaggcc ttgcgcgcgc ccttgaaggt gctcgatcag gcgctcgcgg cgggaggcgg
     1501 cttcgtggtc ggacggcgct tcaccgtcgc cgacatcaat gtcgccgagg tcgtgcgcta
     1561 cgccttgccg gcaacggagc tgctcgccga ggcgccgaac gtcaaggctt ggatcgccgc
     1621 ctgccacgct cgacccgcct tcaaggacat gatggcaaag cgcgagctgg agcccgcgtg
     1681 atgaacgcga agccctatca cccgatcttc tcgcccgagc cgcgccatca caccgaggcc
     1741 gagttctcgc gcgaggaggt catgctcgcg caccacaaca acgccttccc gctcgaggca
     1801 ctcgcgcacg acgtgacgcc tgcagggctg cactacgtgc tcacgcactt cgacattccc
     1861 tatgtccccg atgccgcggc gtggcggctc gcggtactcg gcgcggtgcc gaacgcggga
     1921 tcgttctccg tcgccgacat ccgcgccatg ccgcagcgca cgctccgcgt gacgctcgag
     1981 tgcgcgggca acggccgcat ccatatcaag ccgcgctggc gctcgatgcc gtggagccac
     2041 gaggccgtct cgacggcgga atggaccggc acgccgctga agaacctgct cgcggccgtc
     2101 ggcgtgaagg acaagtttgc gtcgggcgtc gtcgaggtgt ccttcctcgg cgtggatcgc
     2161 ggtttcgaca agggggtcga gcacaacttc ggccgctcgc tgaagccggc gatggcgctt
     2221 tccgatgacg tgctcgtcgt ccatgccatg aatggccagc ctttgcttcc gcagcatggc
     2281 tttccgctgc gcctgatcgt tcccggatgg tacggcatgg cgagcgtcaa gtggctcgat
     2341 cggatcgaag tcctgacgag gccgttcgac ggcttccagc aggtcggcac ctatcatttc
     2401 cgcgacaggt cgggcggggc cccggtgccg gtcacgacga tgcgcgtgaa gtcgctcatc
     2461 aagccacccg gaatcccaga ctggtactca cgcgcgcggc tcctcgatcg cggtcgcgtc
     2521 gtgctcgaag gccgcgcctg ggccggtggc gggcgcgcca tcaagagtgt gcttgttgcc
     2581 atcgatggcg tgctgcgcgc ggctttcgtc catccgaaac cgggtccgtt tgcctggcag
     2641 aagtggacgt gcgagtggga tgcgacaccc ggcgagcaca tattggcctg ccgggccatc
     2701 gacgacgccg gtgccgagca gccgctcgat gcgccctatg atgccggcgg cttcggcaac
     2761 aatggcgcac acatgatgac cgtcacggtc agatagctgg gaacgtcacg ggaatggccg
     2821 atacgatcga agtgcacgta tggcgcggcg aggaaaccgg ccgctatcag gtgttcgagg
     2881 tgccgctgcg cgagagccag accgtgctcg accttgtgac ctgggtgcag cggcacaccg
     2941 acccgacgct cgcttatcgc ttcgcctgcc gcgtcggcat gtgcgggagc tgcgccatga
     3001 ccgtcaacgg ccggccacgc tggacctgcc gcacgcacgt ctcgaaggtg atcgagaacg
     3061 gccgcatcga gatcggcccg ctcgcgaacc tgcccgtcat ccgcgacctc gtaaccgaca
     3121 tgacgcgctt cttcgagaaa tggcaggcgg cgaaaggcgc tttcgagccg tcgaagtcgc
     3181 gccacgacgc gctcnnnnnn nnnnagccga tcagcccgca gacgaaagag cggatcgccg
     3241 ccgacgaggc gatcgagtgc atcaactgcg gcgtctgcta cgccgcctgc gatactgtgc
     3301 gctggaacga ggactatctc ggtcccgctg cactcaaccg ggcctggacg ctcgtcaacg
     3361 acgtgcgcga tgccggcaat gccgaccggc tctgggccgt ctcgcgcgac ggcggctgcc
     3421 acaactgcca ctcgcaccag agctgcgagc agcattgccc gaaggcgctg aacccgacgt
     3481 cggcgatcgc cggcctcaag cggcgcacca tgcaggccta catccggggc gagctcaaat
     3541 gaacgtccgc ctctacgtcc tccagcgcct cactgcgacc gcgatggtgc cgctgatcgc
     3601 gatccacctc gcgactatga tctatgccat ccgcggcggg ctcaccgcga cagagatcct
     3661 ctcacgcacg cgcggcagtg tcggctgggc gctgttctac gggctcttca tcatgctcgc
     3721 cgcggtgcac ggcgcgatcg gcgtgcgcgg cgtgctccgg gaatggtcga ggctcgatgc
     3781 ccgcgcgctc gatctcgtga tgtggggcgt tggtctcatc ctcgtgctgc tcgggctccg
     3841 cgccgtcgcc gcggtggtgc tgccatgagg cggccgtcga attcagccta tcgccgcgac
     3901 acgctctggc tcaccgccat ggtgcatcgc gtctcaggag tcctgctcgc ctgcttcctg
     3961 cccttgcatt tcctggcgct cggcctcgca atcaatggcg cggcccgcct cgacggtttt
     4021 ctcaaatgga ccgacaaccg atgggtgaag gccgccgagg ttggcctcgt cctcctgctc
     4081 gccgtccatc tcctcggcgg cttgcgcatc cttgtcatcg agaacatgcc gtggcgtgac
     4141 gggcagaagc ggatggcgct cggcgcggtc gccattgcga cggtcgtcgc cggcgccttc
     4201 ctgataggtg cgctcacttg aggcggcttc tgcaactcgc attatcgccc ccgaaagaca
     4261 ttattgctaa tatcgaccgc gtgaggccgt ctcaagggcg cgagcgagca ggtcgtgatg
     4321 gcgtatgcga tcaatgtcat ggcaggcgcc gccgtgctct acggcggtat cgtgctcgcg
     4381 gcctggcttg ggcagcgcaa gctcatgtac cacccgaaca cgacccgcac ggcacccgcg
     4441 gcagtcggcc tcgtcggggt cgaagagcgc ctcgtcgaga cggacgacgg cgcccgtatc
     4501 gtgacttggc aggtgaaggc cgagccgggc cgtccgacga tcctctactt ccacggcaat
     4561 gcgggcgacc tcgccgaccg ggccgagcgt atccggcagt tcagcgcggc cgggtggggc
     4621 gccgttatga tggcctatcg cggctatgcg ggttcgaccg ggtcgccctc tgaagctgcc
     4681 aatgtcgccg atgcgaagcg cgtcttcgac cggctcgtcg cagagggcac gcgcgccgag
     4741 agcatcatcg cctatgggga atcgctcggc tcgggcgtgg ccgtgcaatt ggccgccagc
     4801 cgcccggtcc gcgcgctcgt cctcgatgcg ccctacacct cgatcgtgga cgtggcagcg
     4861 agcgtctatc cgtatctgcc ggttcgtcca ctgctgaagg accgctacga atcgagccgc
     4921 cacctcggcg tgatcaaggt gccggtgctc atcctgcacg gtgagcgcga tcaggtcatc
     4981 ccgatcgcca tgggccgcgc catgtacgcg ctcgccaacg agccgaagcg catggtcgag
     5041 tttccggctg gcaaccactc ggacctcttc agccacggtg ccctcgaggc gatccgccgc
     5101 tggctcggcg aggtgggtgc ggcagagccg gcacggtgag ccgctacccg atcaggcctg
     5161 gcgctcagcg gcacgtgctt tctcccactc cgccctcagc cgcatgatgg ggatcgaggc
     5221 caggtaggcg agcagcgtcc agcccatcac gaggaaccag aaaacgtcgc tgatcttcgc
     5281 cgcctcggcc acggacttga cgaacatgaa gaggccgaag aaggcgccgg tcgataggcc
     5341 gagcgagatc gtttgcgctc gtgtcatcgg cacagctgcg atggccttgt agggtccgcc
     5401 gaccttctta gcgagccagc ggttgaacgg catgtaggtg acggcgaaga gggcaccaaa
     5461 gacgatcccc gagaggagcg ggcccggaat catccagagc acgaaatttg cggtcagcat
     5521 cggcagggct cccagattgt gaacagctgt tcattgcggc tgccagagca agtctggcac
     5581 cgcaccttgg tccggccaat cgcgtgaggc gttgccttgc ggcccgcagc cggctcatga
     5641 tgggcgtgtc gcgaggcgcg agcaagccgt gaggaggcgc gcatgtctgg catcgctcgt
     5701 ccgccctcgg tggtcgtgct gacgtcggtt tcagcccaga ttttcgtcgc cgacgtccgg
     5761 cgatcgagtg agttctacct cgagaagctc gggttcacgg tcgacttcct ccatggcgag
     5821 cccccgttct acgggcaggt gcagcgcgac cgtgcgctga ttgccatgcg cctcgtctgc
     5881 gagccggtgt tcgccggcga catccgcgat cgcgagcagc tcctctctgg cgcgatcacg
     5941 gtcgagacgg cgaccgcgat tagagtgctc tttgcagact atgaggccaa gggtgtcgac
     6001 ttccagcagc ggctggccga gcagccctgg ggtgccttga acttcgtcgt gcgcgatccc
     6061 gacggcaacc tcatcctgtt cgcgggccct gcacgctgac gtccggtgcc ccgaacggca
     6121 caatccgcct ccgaaggccg ctgtctctaa gaccaattgc tgttgtcacg cggctctgct
     6181 cttctctgcc tgatctgttg cgccatcgag gagacccaca tgaagacgcg gacatcggcc
     6241 ctggcactcg ccggcgccct gacggccttt gcgctcggcg cgatcgccta tgcgcaagag
     6301 aagcgcgcgg ccgatgccgg cgggctggtc aagaaggagg tcttcaccat ggagacctac
     6361 aagaccgtca gcggcaagga ggtgaagaag ctcaaggccg gttggcaggc gatgggcacg
     6421 ctcaacgccg acaagtcgaa cgcgatcctc gtcacgcact tcttctccgg cacgagccac
     6481 ttcgcgggga agtacaaggc cgaggacaag gctgcgggct actgggacgc gatcatcgga
     6541 cccggcaagg ccgtcgacac gaacaagtac tacgtgctgt cgatggacac gctcgtcaat
     6601 ctcaatgcca aggacccgat ggtgaccacc acgggaccgg cctcgatcga tccggcgacg
     6661 ggcaagccct acgccatgaa attcccgatc gtgacgatcc gcgatttcgt caacgtgcag
     6721 aaggcactcc tcgaccagct cggcatcaag aagctgcacg ccgtgatggg cgcctccatg
     6781 ggtgctctcc aggcctacga gtgggcggcg agctacccgg acatggtcgg ccgcgtcatc
     6841 ccggtgatcg gcgcaggtga atccgacggc tacctgatcg cctggctcga catgtgggcg
     6901 gcccccatca aggtcgacgc caactggaag aacggcgatt actacggtgg ccccgagccg
     6961 accgcgggtc tcgccgcggc gctgaagatc gtgacactgc acgcccagca ctgggcctgg
     7021 acgaactccg ccccgttcgg gcgcaagtgg gcggcagccg acaagaaccc cgccgactgc
     7081 atgctcgact gccgctatgc gatcgagacc gtgctcgacg gcgcgggccg tgcgcgggcg
     7141 gcgatctcgg acgccaacca cttcctctat ctcgtcaagg cgaaccagtt gttcgccgcg
     7201 ggcggcaaca tcgaggaggg tctgaagaag atcaacgcgc cggtgctcct catcacgacc
     7261 gacgacgacc tcgtcttcca cgtcgacggc gtgaagcgga cggtcgatct gatcaaggcg
     7321 gacggcacgc ccgtcgagca cgtcaagatc aagggcggtc gcgggcacct cgacggcgtc
     7381 ctgcacatcg gccaggccgc ggcccagctg acggccttcc tcgccaaata actgccggag
     7441 catggcccca tccggccgag ggcacctcgt gtcggcttgg ggccaggcaa atggctcatg
     7501 gggggctcca cttcttgttc cctctccccg ttgcgcaact tcagcgcgtg ggggattggg
     7561 ccagggtgag gggcggccac aggcactgtc gatgcgtgag ggagcgacgc cattgaaggg
     7621 caagcagccg tgccgaagcc cgcagaggcg ggaaggacga cgtcgcgaat gaaccagagc
     7681 ggcccaacaa ccacacgccg aagcccgggt tcacggcgcg gccacgcatt gttcggcctt
     7741 ccgacacgac aacccgtacc atggcgatgc gttccgctcg gtgcggcaca atcctgcgtc
     7801 tcgctcaccc acgtcgccgg cggctcgaac gtgccgagcg cggggccgtc ctgcgatcgg
     7861 cctgacgatc acggcgcttc ctggatgcaa acggacgagt tgacatctcg acgcccgagc
     7921 ccagaatgag cacagatgca gcgtggcgac aaaggaaaga gccaaccaaa tgatccttcc
     7981 ccagaaattc agatcataca cgatggcgat tctgggcctg gccgggacgg gggcgctcgc
     8041 cgtctcgagc cccttgtgga ccggctcgcc ggcgatcgcg caggtgacct tcggcagcct
     8101 gacctgcgac tacgagagga aggtgcgctc gaccgattcg agcacggcga cgaccatcac
     8161 gttcatcaac aaggcaaaga catatcgcgg cctgaactgg atcgacttca agggccagca
     8221 gaagagcttt ggcggcttga atcccggcga gcgcaggacg atcagcacct accgcaccca
     8281 cccttgggtc gtcacgaccg gccccggcga ctgcatcaag atctacttgc cgacggcgtt
     8341 tcccgcgacg gtgaccctga ggtagtccgc agagttgatc ggggcgtgcg gcggaaggtg
     8401 caacccgctg ccgcccctcc cgcttggccg agcctttccg ttcgatcggt cacgaccccg
     8461 ccaccccggc gcagctcaag acgtcaggcg acttgacctc ggtctggctt gggttggcgt
     8521 cgcaggagca ggcgccgtct gcccgccggt gtagcctcga gttggcccaa ggccgcacac
     8581 ggcgaacaca accccgatca gagcacgtcc ctcaattggg acatcccgtc ttgggtcgtg
     8641 tcttgctact tctgcacctg atatcattga tctcagcgag gcactgcttg tggttcgcaa
     8701 gacaaacccg ttggttgccc gcgcagattt tgcggcaata ctcccggcgg tcgggcaccg
     8761 tgatcgccct gcactccagc tcgcattttt gagccgagtt ggtgcacctg gcgagcattc
     8821 ggctaggatt gagtccaccg caagcattca cttgaatcac atcgccgctg ctccgaacac
     8881 cgggatccaa gaccggcatg cttgcttgcg atggcgcctg cgatagggcc agcaccagtg
     8941 cacctgcggc tgcgagtgag agaatgagag atttcatcga gtgccactcc atccaagttg
     9001 tccaacatga gcataggggc acggacgtgc gcgataggca aatgctctct gagacgagtg
     9061 ctgcatcgat gccgagccag cgcatcgcgc ggcagcgaac cggacgccgc gtcacgaggt
     9121 ccggaaatta agcatcgcga ccggttgagc gaggcgacgg accggggcgg cgctacgatc
     9181 cgggtaacct gttgtttacc ctggagcaaa atatgatctc gcgccgcctt ctccgtatcg
     9241 ccctgccgac cgcgggtctc gcggccttgg cgaccaccgc acttgccggt gagccctcga
     9301 ccacccgcgt cgagacgcgc gccttctacg gcgcgaccgt caccatcgag gagggcgtg
//
LOCUS       DAORNH010000179         9329 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5468_length_9329_cov_3.005391, whole genome shotgun sequence.
ACCESSION   DAORNH010000179 DAORNH010000000
VERSION     DAORNH010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5468_length_9329_cov_3.005391"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1146
                     /locus_tag="PK264_16585"
     CDS             205..1146
                     /locus_tag="PK264_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRD77524.1"
                     /translation="MSRLPKHVTIVEVGTRDGFQIEKKFVPTDVKVEIINGLLAAGVQ
                     QMQVTSFVSPKAVPALADAEEVFRRINRPKGVTLTALVANMKGAERAIAAKVDVLDLV
                     VSASETHNRKNVNRSVDESLQGFTEIAKVAAAAGVAIKGGIATALGCPFEGDVPTAQI
                     VRIARHYRDLGAKFVSLGDTTGMATPPIVRKAVRALKAEVPEVEVVLHLHNTRGLGLV
                     NAMAGLEEGVSKLDGAIAGLGGCPFAAGATGNVCTEDLVFMLHECGIDTGIDLRKLIA
                     VAKRVQDVFGRELPGQVMKSGLRLDLHDTKAVPTAAG"
     gene            complement(1165..3222)
                     /locus_tag="PK264_16590"
     CDS             complement(1165..3222)
                     /locus_tag="PK264_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD77525.1"
                     /translation="MAGWRVGVDSGGTFTDICLVDDTTGDVRVWKVPSTPDDPSRAIA
                     AGSAEGLARAAGVGPDRTSYFGHGTTVATNALIQHRGVEVGLITTEGFRDLLEIGRQR
                     RPHLYDLQTEKPPLLVRRDRRIEVPERLRFDGSVATRLDEAAVREAARRLKAMGVRSV
                     AICFLYAYINPAHEQRAVAIVAEEFPEAFVTASHDVAPEFREFERLSTVVVNAYLGPV
                     LGRYVESLGPRLDQAGFKGPRHITQSNGGVMSLATASRQAVRTVLSGPASGVVGASEI
                     GRLSGFRNLITFDMGGTSTDVALIEEGRTRLAGEMDVHGYPLKTPMLDIHTVGAGGGS
                     IAHIDAGGHLKVGPRSAGSVPGPVCYGLGNAEPTVTDANVALGTLHPTHLLAGRMPID
                     RAKSLAAIAALSTRLGLDANATADGIISVVVANMAKAIRVISVQRGHDPREYVLVAFG
                     GAGPLHAGRLARELDMGTILVPRNPGILCALGLLLSDLKTNYSITRQMALDPERGSDI
                     TSAFAELETRAGRWFDEERIAATDRRLIRTVDMRYAGQNYELPVAMPEALAGAELVAA
                     LRAGFESAHRQMYGYAADGEKIQAITFRLEATGKVRATNLRAHAGTTASTEAARTGVR
                     EVWVQSAGAFIAHPVYDREKLSPGHVIAGPAIIEQMDATTVILKSQSALVDAYLNIIV
                     SES"
     gene            3418..4473
                     /locus_tag="PK264_16595"
     CDS             3418..4473
                     /locus_tag="PK264_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD77526.1"
                     /translation="MMRARLVHGLAVAALLAGAGLMAGSAGTKAQTKSFFVAGYGGSF
                     EQTMRKEIIPAFEKKHGVKVEYVAGNSTDTLAKLQAQKGNQQIDVAIVDDGPMFRAIE
                     LGFCKKIDGLPEADLYPAARFKGDKAVAIGLVGTGIMYNTKYFKDKGWAPPTSWNDLA
                     DPKFKKLLVIPPINNTYGLYTLMMFARMNGGGEKNIEPGFKAMIEKINPNVLVYEPSP
                     GKMTELFQSGQAVIAVWGTGRVQSFANTGFPVDFVYPKEGTVTLLASACPVEKPNASP
                     IAFDFIKMMIEPALQLVLLKDYGYGPVNNKVTVPADLGKMAPIGERAKLLVNPDWDAI
                     NPNRDEWTKRWNRQVER"
     gene            4585..5652
                     /locus_tag="PK264_16600"
     CDS             4585..5652
                     /locus_tag="PK264_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD77527.1"
                     /translation="MPLLVLDKVTKHFGDQVAVEGLSLAVEQGEFVSLLGPSGCGKTT
                     TLQMIAGFADVTAGRITLEGRDLTSVRPAERGLGIVFQSYALFPHMTAAENVAFGLEM
                     QGVQAAERARRVSETLALVGLAGFESRFPRRMSGGQQQRVALARAIVIKPRILLLDEP
                     LSNLDAKLREEMQIELRQIQRTLGTTTILVTHDQSEAMALSDRIVVMSKGRVEQIATP
                     EQLYDRPETHFVRTFLGKMNVLTVPALADGRGSTLKVGEGRVSSERPAADFPNGLARL
                     SVRPERVRIVDAGIGVPGKIAMRVFQGNHWLVRIALAPDLDMMAIVPNMGRPMPAEGA
                     PVGLAWEPGDMHIEAASGGSP"
     gene            5649..6530
                     /locus_tag="PK264_16605"
     CDS             5649..6530
                     /locus_tag="PK264_16605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77528.1"
                     /translation="MSAPAETTRHDGARPYLLAAPGLLLFVGVVLIPLAMTVLLSFHD
                     WGQTKGVEPVLILKNWREIAADSYFHEMFLRTFRIAFLVTIITALLGAPEAYILHRMR
                     APWKGLFLLVVIGPLLISVVARTLGWALLFGGNSGLVNKVLMDNKIIASPIPFMFTET
                     GVVVALAHVLMPFMVLSVWASLQRLDPQVENAAASLGASQPTILRRIVLPQVMPGVLS
                     GAIIVFALAASAFASPAIIGGRRLKVASTLAYDEFLNTLNWPLGAAVAVLLLIAIAAV
                     IVGSNRLVERRYQEVFR"
     gene            6527..7336
                     /locus_tag="PK264_16610"
     CDS             6527..7336
                     /locus_tag="PK264_16610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77529.1"
                     /translation="MRRNGPFALLFHTLFVIFMLAPIAIVCVVAFTPEGFLSLPTNGP
                     SLRWFRAIWNYPEFVRAFWVSIWLGAVSSAIAVAIAVPAAIAIARHRFRGRDVIAAFF
                     MSPLMIPHVVLGIAFLRFFTQVGIGGQFIGLLLAHVVVVFPFALRLTLAAATGIDRTI
                     EQAAVSLGAPDRTVFRRITMPLILPGIASGWALAFIQSFDELTMTVFLATPGMETLPV
                     RMFLYIQDNIDPLVTSVSAAVIAVTTVFLVILDRIYGLERLLAGRSAADGR"
     gene            7326..8465
                     /locus_tag="PK264_16615"
     CDS             7326..8465
                     /locus_tag="PK264_16615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD77530.1"
                     /translation="MADEATSRDYDVAVVGGGLLGASIAWGLARRGERVVVLDEGDIT
                     KRASRANFALVWVQSKGLGMSEYTQWTVRSSKAWPRLADALKQQTGLDVALQQPGGLH
                     LALSDGELEKRRDMLRRLHNQPGAADYKVEILDRRAVERMLPGVGPDVVGGTYCPLDG
                     HVNSLRLFRALHTGFRMLGGTYLPEHAVSGIEPLAGGGFRIATENGAVSAGKVVLAAG
                     NANRELAPLVGLSAPMKPNRGQIIVTERLKPFLHYPVTTLRQTDEGTVMIGDSLEDRT
                     VDTGMTLPINAVMADRARRMFPMLARANVVRTWAGVRVMTADGFPIYNQSESCPGAFV
                     ACCHSGVTLAANHADEIAGQIAAGRLDPALVGAFSAERFDVQTAA"
     gene            8446..8772
                     /locus_tag="PK264_16620"
     CDS             8446..8772
                     /locus_tag="PK264_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD77531.1"
                     /translation="MFRRLPDGKTGTIALTIDGAMVIARAGDSVAAALLLAGLDHCRE
                     TPVKGSRRQPYCMMGVCFDCLVTIDGVANRQACLIPVREGMKVERQIGARDIAAPSTG
                     STEGRA"
     gene            8769..>9329
                     /locus_tag="PK264_16625"
     CDS             8769..>9329
                     /locus_tag="PK264_16625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRD77532.1"
                     /translation="MKDAATSQHTHDVIVIGAGPAGMAAAAATAMAGLSTLVLDENRG
                     PGGQIYRAITASPPSDASILGDDYWEGARLAEEFGGSSAEVTYGATVWTLDRELVVGV
                     SKGGVSDLLSARRVVIATGALERPFPIPGWTLPGVMTAGAAQTLLKSSGVVPKGRIVL
                     AGTGPLLWLLGAQLLRAGADVTAILDT"
BASE COUNT         1525 a         3231 c         3002 g         1571 t
ORIGIN      
        1 tacggagccg gccaaggtcc gccgcgccga cgccgtcgat ccgaagaacc ccgcgacctg
       61 gggcaaagtc tcgcgcaacg acaagtgccc ctgcggctcg ggcaagaaat acaagaagtg
      121 ccacggcgcc gatcgcgact gatcgtgtga tccgatctgg ggccgatgcg agagcgtcgg
      181 ccccttctca attgcgaggt ccccatgagc cggctgccga agcacgtcac cattgtcgag
      241 gtcggaaccc gcgacggctt ccagatcgag aagaagttcg tgccgaccga cgtaaaggta
      301 gagatcatca acggcctgct cgcggccggc gtccagcaga tgcaagtgac ctcgttcgtg
      361 tcaccgaagg cggtccctgc gctcgcggac gccgaggagg tgttccgccg catcaaccgg
      421 ccgaagggtg tcacgctcac ggcactcgtc gccaacatga agggcgccga gcgcgccatc
      481 gccgccaagg tcgacgtgct cgatctcgtc gtctccgcct ccgagacgca caaccgcaag
      541 aacgttaacc gctcggtcga cgaaagcctt caaggcttca ccgagatcgc caaagtcgcc
      601 gcggcggccg gtgtcgccat caagggcggc atcgcgactg ccctcggctg ccccttcgag
      661 ggcgacgtgc caaccgcgca gatcgtgcgc atcgccaggc actaccgcga tctcggcgcc
      721 aagttcgtga gcctcggcga tacgaccggc atggcgacgc cgccgatcgt gcgcaaggcc
      781 gtccgcgccc tgaaggcgga ggtacccgag gtcgaggtcg tgctgcatct ccacaacact
      841 cgcgggctcg gcctcgtcaa cgccatggcc ggcctcgagg aaggcgtcag taagctcgac
      901 ggcgccatcg ccggtctcgg cggctgcccg tttgcggcgg gcgccactgg taatgtgtgc
      961 accgaggacc tcgtgttcat gctgcacgag tgcggcatcg acacgggcat cgacctcaga
     1021 aagctcattg cggtcgcaaa gcgcgtccag gatgtgttcg gccgagagct gccgggccag
     1081 gtgatgaagt caggcctccg cctcgacctc cacgacacca aagcagtacc gaccgcggcc
     1141 ggctgataag ccgaaacccg gcgctcagct ctcgctcaca atgatgttga gataggcatc
     1201 gaccagcgcc gattggctct tgaggatcac cgtcgtcgcg tccatctgct cgatgattgc
     1261 cgggccggcg atcacgtgac caggcgacag tttctcgcgg tcgtagacgg ggtgcgcaat
     1321 gaacgcgccc gcgctctgca cccagacctc gcgcacgccc gttcgcgccg cctccgtcga
     1381 tgccgtcgtg cccgcgtgcg ccctcagatt cgtcgcacgc accttgccgg tcgcctcgag
     1441 ccggaaggtg atggcctgga tcttctcgcc gtcggcggcg tagccgtaca tctgccggtg
     1501 cgcgctttca aagcccgccc tcagcgcggc gaccagctca gcgcctgcaa gcgcctcggg
     1561 catcgccact gggagctcat agttctgccc cgcatagcgc atgtcgacgg tccgtatcag
     1621 ccgccggtcc gtcgcggcaa tccgctcctc gtcgaaccag cgcccggcgc gcgtctcgag
     1681 ctccgcgaac gcgctcgtaa tatcgctccc gcgctcggga tcaagcgcca tctgccgtgt
     1741 gatcgagtag ttcgtcttga ggtcgctcag gagcaggccg agcgcgcaca gaatgcccgg
     1801 attgcgcggc accaggatcg tgcccatgtc gagctcgcgt gcgagccggc ccgcatgcag
     1861 cggccccgca ccaccgaaag cgacgagcac gtactcacgc ggatcatgcc cgcgctgcac
     1921 cgagatcacg cggatcgcct tcgccatgtt ggcgacgacc accgagatga tgccatcagc
     1981 cgtcgcgttg gcatcgaggc ccaagcgagt gctaagcgcc gcgatggccg cgagggactt
     2041 cgcccggtcg atcggcatgc ggccggcgag cagatgtgtc gggtgcagcg tgccgagcgc
     2101 tacgttggcg tccgtcaccg tcggctcggc attgccgagc ccgtagcaga cgggtcctgg
     2161 aacgctgccc gcgctgcgcg gcccgacctt caggtgcccg cccgcatcga tgtgggcgat
     2221 ggagccaccg ccggcgccga ccgtgtggat gtcgagcatc ggtgtcttta acggatagcc
     2281 gtgcacgtcc atctctccgg cgagtcgcgt gcgtccctcc tcgatcagtg cgacatcggt
     2341 cgaggtgccg cccatatcga aagtgatgag attgcggaag cccgacagcc tgccgatctc
     2401 gctcgccccc accacgcccg atgccggtcc cgatagcacc gtgcgcaccg cctgccggct
     2461 cgcggtcgca agcgacatga cgccaccgtt cgactgcgtg atgtgacgcg ggcccttgaa
     2521 accggcttga tcgaggcgcg gaccgaggct ttccacatag cggccgagca cggggccgag
     2581 ataggcgttg acgacaacgg tcgacaagcg ctcgaactcg cggaattcgg gcgccacgtc
     2641 gtgcgaagcg gtgacgaacg cctccgggaa ttcctcggcg acgatggcga ccgcgcgctg
     2701 ctcgtgtgcg ggattgatgt aggcatagag aaagcagatc gcgaccgagc gaacgcccat
     2761 cgccttcaac cgtcgtgccg cctcgcgcac cgctgcctca tcgagccgcg tcgcgaccga
     2821 gccgtcgaag cgcagccgct ccggcacctc gatccgccgg tcacgccgga cgagcagcgg
     2881 cggtttctcg gtctggaggt cgtagaggtg ggggcggcgc tggcggccga tctcgaggag
     2941 atcgcggaag ccctccgtgg tgatgagccc gacctcgacg ccgcgatgct ggatcagggc
     3001 attcgtcgcg accgtggtgc cgtggccgaa atagctcgtg cggtcaggcc cgacaccggc
     3061 cgccctggca agcccctcgg cgctgccggc cgcaatggcg cgcgagggat cgtccggagt
     3121 cgatgggacc ttccagacgc gtacatctcc cgtcgtgtcg tcgacgaggc agatatccgt
     3181 gaacgtcccc cccgaatcca cgccgacccg ccaacccgcc atcctgccga tccttcgagc
     3241 gattgctgag attttcgcca gagagcaagg cacggcgagc aactgtcaag cctcgcggcc
     3301 agttcaccgt cccggcagac gcatggctgg ccgtcgcggc agcagttccg aattgacgta
     3361 tcgcgtaccg ggatggaact ttattggaag actggacgtc cgcaacccag ggaggagttg
     3421 atgcgagcgc gactggtgca tggattagcc gttgccgcac ttttggccgg cgcggggctg
     3481 atggccggaa gtgccggcac gaaggctcag accaaatcgt tcttcgtcgc cggctacggc
     3541 ggctccttcg agcagacgat gcgcaaggag atcatcccgg ccttcgagaa gaagcacggc
     3601 gtgaaggtcg agtatgtcgc cggcaactcc accgacacgc tcgccaagct gcaggcgcag
     3661 aagggaaacc agcagatcga cgtcgccatc gtcgatgacg gcccgatgtt ccgcgcgatc
     3721 gaactcggct tctgcaagaa gatcgacggg ctgcccgaag cggacctcta cccggccgcg
     3781 cgcttcaagg gcgacaaggc cgtcgccatc ggcctcgtcg gcaccggcat catgtacaat
     3841 accaagtatt tcaaggacaa gggctgggct ccgccgacat cgtggaacga cctcgccgac
     3901 ccgaagttca agaagctgct ggtcatcccg ccgatcaaca acacctacgg cctctacacg
     3961 ctcatgatgt tcgcccgcat gaacggcggc ggcgagaaga acatcgagcc gggcttcaag
     4021 gcgatgatcg agaagatcaa tccgaacgtg ctcgtctatg agccatcgcc tggcaagatg
     4081 acggagctct tccagtccgg acaggccgtg atcgcggtct ggggcacggg ccgcgtgcag
     4141 tcgttcgcca acaccggctt cccggtcgac ttcgtctacc ctaaggaagg tacggtgaca
     4201 ctgctcgcct cggcctgccc ggtcgagaag cccaacgcct cgccgatcgc attcgacttc
     4261 atcaagatga tgatcgagcc cgcattgcag ctcgtcctcc tcaaggacta cggctacggc
     4321 cccgtcaata acaaggtcac cgttcccgcc gaccttggca agatggcgcc aatcggcgag
     4381 cgcgcaaagc tcctcgtcaa tcccgactgg gacgccatca atcccaaccg cgacgagtgg
     4441 accaagcgct ggaaccgtca ggtcgagcgg taagcacgtt gcgcgggatg ttgcagcctc
     4501 cttcccacga gcgacgtggg taggaggccg ttgcgtgtcg tgaccggcgc ggtgatgcaa
     4561 atcactgcca tttccgaggc ttgaatgccc ctcctcgttc tcgacaaggt gacgaagcac
     4621 ttcggcgacc aggtcgccgt cgaggggctg tcgctcgctg tcgagcaagg ggagttcgtc
     4681 tcgctgctag gaccctcagg ctgcggcaag acgacgacgc tgcagatgat tgcgggcttc
     4741 gccgacgtga ccgcgggcag gatcacgctg gagggccgtg atctgacgtc ggtgcgccca
     4801 gccgagcgcg gcctcggcat cgtattccag agctacgcgc tctttcccca catgacggcg
     4861 gccgagaacg tcgccttcgg cctcgagatg caaggcgtgc aggcggcgga gcgcgcccgc
     4921 cgcgtctccg agacgcttgc actcgtcggc ctcgccggct tcgagagccg cttcccgcgc
     4981 cgcatgtccg gcggtcagca gcagcgcgtg gcgctcgccc gcgccatcgt catcaagccg
     5041 cgcatcctgc ttctcgacga gccgctctcg aacctcgatg ccaagctccg tgaggagatg
     5101 cagatcgagc tccgccagat ccagcgcacg ctcggcacga cgaccatcct cgtcacgcac
     5161 gaccagtccg aggccatggc gctctcggat cgcatcgtcg tcatgagcaa gggcagggtc
     5221 gagcagatcg ccacacccga gcagctctat gaccggcccg agacgcattt cgtgcgcact
     5281 ttcctcggca agatgaacgt gctcaccgtg ccggccctgg ccgatggccg gggcagcacg
     5341 ttgaaggtcg gtgaagggag ggtttcgagc gagcggcctg ccgccgactt tcccaatggc
     5401 ctcgcgcgcc tctccgtcag accggagcgc gtgcgtatcg tcgacgccgg catcggcgtc
     5461 ccgggaaaga tcgccatgcg cgtcttccag ggcaaccact ggctcgtgcg catcgcactg
     5521 gcgcctgatc tcgacatgat ggcgattgtg ccgaatatgg gccggcccat gcccgcggaa
     5581 ggcgcgcccg tcggcctcgc ctgggaaccg ggcgacatgc acatcgaggc agccagcgga
     5641 ggcagcccat gagcgccccc gcagagacga cccgccacga tggcgcgcgg ccctatctcc
     5701 ttgccgctcc gggcctcctc ctcttcgtcg gcgtggtgct gatcccgctc gccatgacag
     5761 tcctgctgtc gttccacgac tggggccaga ctaagggcgt cgagccggtg ctcatcctga
     5821 agaactggcg cgagatcgcc gccgacagct acttccacga gatgttcctg cgcacgttcc
     5881 gcatcgcatt cctggtgacg atcatcaccg cgctgctcgg cgcgcccgag gcctacatcc
     5941 tccaccgtat gcgagcgccg tggaaggggc tgttcctgct cgttgtgatc gggccgctac
     6001 tgatctcggt cgtcgcccgc acactcggct gggcactgct cttcggtggc aacagcggcc
     6061 tcgtcaataa agtcctgatg gacaacaaga tcattgcctc gccgatcccc ttcatgttca
     6121 ccgagacagg tgtcgtcgtg gcgctcgccc acgtgctgat gccgttcatg gtgctctcgg
     6181 tgtgggcctc cctccagcgc ctcgacccgc aggtcgagaa cgcggctgcc tcgctcggcg
     6241 ccagccagcc gacgatcctg cgccgtatcg tgctgccaca ggtgatgcca ggcgtgctct
     6301 cgggggcgat catcgtcttc gcgctggccg cgagcgcctt cgcctcgccg gcgatcatcg
     6361 gcgggcggcg gctgaaggtc gcctcgacgc ttgcctacga cgagttcctg aacacgctca
     6421 actggccgct cggtgccgcg gtcgcggtgc tgctgctgat cgccatcgcg gcggtcatcg
     6481 tcggctcgaa ccggctcgtg gagcggcgct accaggaggt gttccgatga ggcgcaacgg
     6541 gccgttcgcg ctcctgttcc acacgctgtt cgtgatcttc atgctggcgc cgatcgcgat
     6601 cgtgtgcgtc gttgcgttca cgcccgaagg cttcctgtcg ctgccgacga acgggccgtc
     6661 actgcgctgg ttccgcgcga tctggaacta ccccgagttc gtgcgcgcgt tctgggtcag
     6721 catctggctc ggcgcggtgt cgtcggcgat cgcggtggcc atcgcggtgc ccgcggcgat
     6781 cgcgattgca cgtcatcgct tccgtggccg tgacgtgatt gcagctttct tcatgtcgcc
     6841 gctgatgatc ccgcacgtcg tgctcggcat cgccttcctg cgcttcttca cgcaggtcgg
     6901 tatcggcggc cagttcatcg ggctcctgct cgcccacgtc gtcgtcgtct tcccgttcgc
     6961 gctgagattg acgctcgctg ccgcgaccgg catcgaccgc accatcgagc aggccgccgt
     7021 ctcgctcggc gctccggatc gcaccgtctt ccggcgcatc accatgccgc tgatcctgcc
     7081 gggcatcgcg agcggctggg cgctcgcctt catccaatcc ttcgacgagc tcaccatgac
     7141 ggtgttcctc gccacgcccg gcatggagac gctgcctgtc cgcatgttcc tctacatcca
     7201 ggacaacatc gatccgctcg tcacctcggt atccgcggcc gtgatcgcgg tcacgaccgt
     7261 cttcctcgtc atcctcgatc gcatctacgg cctcgagcgc ctgctcgccg gacggagcgc
     7321 tgccgatggc cgatgaagcg acatcccgtg actacgacgt cgccgtggtg ggcggtggct
     7381 tgctcggcgc atcgatcgcc tgggggctcg cgcgccgcgg cgagcgggtc gttgttctcg
     7441 acgaaggcga catcacgaaa cgggcctcgc gcgccaactt cgcgctcgtg tgggtgcaga
     7501 gcaagggcct cggcatgtcc gagtacaccc aatggacggt gcgctcgtcg aaggcctggc
     7561 cgcggctcgc cgatgccttg aagcagcaga cggggctcga cgtcgcgctg cagcagcctg
     7621 gcgggctgca tctcgcactc tcggacggcg agctcgagaa gcggcgcgac atgctccgcc
     7681 ggctgcacaa ccagccggga gcggcggact acaaggtcga gatcctcgat cgacgcgccg
     7741 tcgagaggat gctgccaggg gtcggcccgg acgtcgtggg cggaacgtat tgcccgctcg
     7801 acggtcacgt caactcgctg cgcctctttc gcgcgctgca cacggggttc cgcatgctcg
     7861 gcggcactta tttgccggaa cacgcggtca gcggtatcga gccccttgcg ggcggcggct
     7921 tccggatcgc gaccgagaat ggcgctgtca gcgccggcaa ggtcgtactc gctgccggca
     7981 atgccaatcg cgagctcgcc ccgctcgtcg gcctctcggc tccgatgaag cccaaccgcg
     8041 ggcagatcat cgtcaccgag cgactgaagc cgttcctgca ctatccggtc acgacgctgc
     8101 gccagacgga cgagggcacc gtgatgatcg gcgacagcct ggaggaccgc accgtcgaca
     8161 cgggcatgac attgccgata aacgccgtaa tggccgaccg cgcgcgccgc atgttcccaa
     8221 tgctcgcccg cgccaatgtc gtgcgcacct gggcgggcgt ccgcgtcatg accgctgacg
     8281 gcttcccgat ctacaaccag tccgagagct gccctggcgc cttcgtcgcc tgctgtcact
     8341 caggcgtcac gctcgccgcc aaccatgccg acgagattgc cggccagatc gccgccggac
     8401 ggctcgaccc cgcgctcgtc ggcgcgttct ccgctgagag gttcgatgtt cagacggctg
     8461 cctgacggca agacgggtac gatcgcactc acgatcgatg gtgcgatggt cattgcgcgc
     8521 gccggcgact ccgtcgccgc cgctctcctg ctcgccggcc tcgatcattg ccgggagact
     8581 ccagtgaagg gcagccgccg ccagccctat tgcatgatgg gcgtgtgctt cgactgcctc
     8641 gtgacgatcg acggcgtcgc caatcgccag gcctgcctca tccccgtccg tgaggggatg
     8701 aaggtcgagc gccagatcgg cgcgcgcgat atcgccgcgc cgtcaaccgg cagcacggag
     8761 gggcgggcat gaaggacgca gccacgtctc agcataccca cgacgtgatc gtcatcggcg
     8821 ccggcccggc cggcatggcg gccgcggcgg caacagcgat ggccggtctc tcgacgctcg
     8881 tgctcgacga gaaccgcgga cccggcgggc agatctatcg cgcgatcacg gcctcgcctc
     8941 ccagcgacgc ctcgatcctt ggtgacgact actgggaggg agcgcgtctc gccgaggaat
     9001 tcggggggag cagcgcagag gtcacttacg gcgcgacggt gtggacgctc gaccgcgaac
     9061 tcgtcgtcgg tgtctcgaag ggcggcgtct ccgatctgct gtcggcccgc cgcgtcgtga
     9121 tcgcgaccgg cgcgctcgag cgaccgttcc ccattccggg atggacactg ccgggcgtca
     9181 tgactgcagg tgctgcgcag acgctgctca agtcgtcggg cgtcgtaccg aagggccgca
     9241 tcgtgcttgc cgggacgggg ccgctccttt ggctgctcgg cgcgcagctc ctcagggccg
     9301 gcgccgatgt cacagccatc ctcgatacg
//
LOCUS       DAORNH010000180         9280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5505_length_9280_cov_2.464715, whole genome shotgun sequence.
ACCESSION   DAORNH010000180 DAORNH010000000
VERSION     DAORNH010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_9280_cov_2.464715"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PK264_16630"
     CDS             <1..644
                     /locus_tag="PK264_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77533.1"
                     /translation="LRLLALLTATTIVTGDVHAQSAATKSDACITDIKAALAKMRDGG
                     PQTHVEEMDTSGQTVRRTSKYMMPKAMSVEGTQTMGEGATPIVLKTHIVIIGDDVWRQ
                     SEGVWKKIDGENAARVVTGVRAAHAKMDEITADEATCDGTETVDGKTLKVYKFTTLVT
                     TMFGAGTTVTAVFVGADGRPARIDSVTSLPNILTKRRNVITYDPAIKIEPPKM"
     gene            668..>819
                     /locus_tag="PK264_16635"
     CDS             668..>819
                     /locus_tag="PK264_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRD77534.1"
                     /translation="MSETPTIDAIRADFALLDDWEDRYRYVIELGRALPPLPEALRTD
                     TNKVRG"
     assembly_gap    820..967
                     /estimated_length=148
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <968..1086
                     /locus_tag="PK264_16640"
     CDS             <968..1086
                     /locus_tag="PK264_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRD77535.1"
                     /translation="VDAQKVFAELGLKEHLTPQRSNGLASMVERIRVDARRA"
     gene            1083..2534
                     /locus_tag="PK264_16645"
     CDS             1083..2534
                     /locus_tag="PK264_16645"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRD77536.1"
                     /translation="MTDVALALLPTVAVLVALLGLRWSGLGAAGLAVAVATALFALGA
                     FRPPTHLVVAQAVPDALLITLLVASMVVPGVLFVIAASRRRASETLAGLVGAIDLPPA
                     SRVIVIALGIGVVVESLTGSGVSLLVTVPLLLRFTDRTRCMSLALIGMSLMPWGALAI
                     SAHVAGGLAGIPVVELTTPIWWLSGCIALILPVLCLVVVGAKQTSPRDVVFAIPAGIV
                     LAGTAGLAGTGIGIETAGVLGGLAVLALGIAASSTRRGLGAALAEPGLRPYLALIVVV
                     LGQKLAIATLVPALGVNPVLSTGRVTFRLLTSPGIPLLIVTLLAMAGSLDLAAVRQMV
                     ERSWRALATVLLFMLSARLMLEIGAFAALARQVAQLGQDMAIVATTLLGAASGFITGS
                     GVSANGLFMPGAAAIGQEFGLTRLFAAIQNCSAGHVAMASLPVGAILLAALPDRQRGD
                     DRHLLVTGLWLAALYTALIAAVAIGAARLGVGA"
     assembly_gap    2932..2985
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2986..3578
                     /locus_tag="PK264_16650"
     CDS             <2986..3578
                     /locus_tag="PK264_16650"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HRD77537.1"
                     /translation="TSDTEIYGVLADLARGSLIGFDPVAAKARIEALPWVERASVGRE
                     LPDRIVIDIRERVPYAVWQTGAELIVVDATGRLLAKSDAASAAKLGLPRIVGDGAPER
                     ARAFHAEIAAFPRLARRLAAAEFVAGRRWTLHMTGGLSVLLPSAGIKETLARLDQLQE
                     RDQILDRAAVTLDLRGSDRLFVDPRPASAAPRRLPG"
     gene            3584..4873
                     /gene="ftsA"
                     /locus_tag="PK264_16655"
     CDS             3584..4873
                     /gene="ftsA"
                     /locus_tag="PK264_16655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRD77538.1"
                     /translation="MLARARPSGEGRIVGLLDIGAGKVCCMIASLSPPAEGANSATVR
                     LLGFGHQRSRGVKAGVVVDLEETVRAVQAAVGAAERMAGLSIDDVYLVVMAGRLKSSL
                     FSAAVQVDRGVVEDEHVARVLAGGEAYLARAGRAIIQHDCVGFRLDGSTNVRDPRGLA
                     GAKLVADLHAVTADEAPLRNLVLAAERCHLTVVGMLSAPAASGLATSTADERELGVVV
                     VDMGAGTTSLAAWSHGRLRHIDVALVGGQHATFDLANALGAPLDEAERIKTRLGTLIA
                     AQSDEREQVSFPLAGNDGLVGPGEGMATRADVRRIIAPRFTRILRDLGQRIAQHPEAR
                     AAASRIVLTGGASHLPGLPECAADILDKPVRIGRPMCAGLPATACQPQFAALIGLLVA
                     LAATPCDGDPMLRAYRDRNVLTGGYIGRLKEWVIGNF"
     gene            4925..6568
                     /gene="ftsZ"
                     /locus_tag="PK264_16660"
     CDS             4925..6568
                     /gene="ftsZ"
                     /locus_tag="PK264_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRD77539.1"
                     /translation="MINLALPRVGGKGPRITVIGIGGAGGNAVNNMIASGLGGVEFIA
                     ANTDAQALETSSAERRIQLGRNLTDGLGAGSKPEVGEAAAEETLDELREVIEGSHMVF
                     LAAGMGGGTGTGAAAVVARTAKELGILTVAIVTKPFVFEGARRMRAADAGIAELKKHV
                     DTLIVIPNQNLFRIANDRTTFAEAFILADQVLLSGIACIVDLIVKEGLINLDFADVQT
                     VMSGMGTAMMGTGEASGPRRAVEAAEEAISNPLIDDVSLQGARGVLLSIIGGRDMTLY
                     EVDEAASRVRQEVDPDANIIVGATFDDSLGDRLRVAIVASGMHQAELQPGAARMAEPA
                     GAPPMPQPMPTNAAGQLFQAHAEHPAFAPHAVPTADTWPAEPAPGPLTTRLRNLPPIP
                     GGPIEAQVRPSSAIFTHSPDARPTIHWQAPGDVMIEDRSAQDADVPSMGDWGAAHPTR
                     PADIPAFTPQPPAEARRTPTPIPPLDHFPAVGQREYRARVLDAEGSEPPIQAPSRHQA
                     PTPAESRGGLLQRLVGRGRSREDAPQKTELPGFFESRRR"
     gene            6826..7779
                     /gene="lpxC"
                     /locus_tag="PK264_16665"
     CDS             6826..7779
                     /gene="lpxC"
                     /locus_tag="PK264_16665"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HRD77540.1"
                     /translation="MSKRFIGARQTTVGREIEIKGTGVHSGAPVSIILHPAPADTGIT
                     FLASKRGKIVSEIPANVSHVKNLTLCTVIGDDRGTTIGTVEHLLSALRGLSIDNCYVQ
                     IDSKEVPIMDGSAAYFVQAIDAVGIRELQSPRKFIKVLKPILVEDNGSVGELSPYSGF
                     HIDCEIQFSSPVIGRQRMALDVNPGTFRNEISRARTFGFMHDVEKLWKAGLALGASLD
                     NTVAIGDDRVMNPEGLRYPQEFVRHKMLDAVGDLTLAGAPILGAYRSVKGGHRLNAHV
                     LQALFADRDAWTMVHAPWVRDGVPVDMPAGASAMNFAADHS"
     gene            8339..9175
                     /locus_tag="PK264_16670"
     CDS             8339..9175
                     /locus_tag="PK264_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HRD77541.1"
                     /translation="MKLQTAFRRKASLALAFAALSGATLLAGCSTSSDMAKVLNPDPP
                     AKMFADADAALARGSWVDAAKKFEDLDRDHPYAPEARRAIVMAAFAYYKAAKFPDAIS
                     AARRYTTMHPGTKEAALAHHIIASAHFDEIQVPSRDQTNTRKALAELKTLRARYPDSP
                     YAKQAENRIRIAEDVLAASEMNVGRYYQKNRNYVAAINRFRTVVVEYQTTTHVEEALF
                     RLTETYMFLGIRNEAQTAAAVLGHAADEVVRQVLLPAHVGEGAVERGENLGLGAVTPC
                     AE"
BASE COUNT         1502 a         3025 c         3014 g         1537 t
ORIGIN      
        1 tcttgcgtct acttgccttg ctcaccgcaa ccacgatcgt aaccggcgac gtccacgctc
       61 agtcggccgc caccaagagc gacgcgtgca tcaccgacat caaggcggca ctcgccaaga
      121 tgcgtgacgg cggcccgcag acgcacgtcg aggagatgga cacgtcgggc cagacggtgc
      181 ggcgcacctc gaaatacatg atgccaaagg cgatgagcgt cgaaggcacg cagacgatgg
      241 gcgagggcgc gacgccgatc gtgctcaaga cgcatatcgt catcatcggc gacgatgtct
      301 ggcgccagag tgaaggggtc tggaagaaga tcgatggcga gaatgccgct cgcgtcgtca
      361 cgggtgtcag agccgcgcac gccaagatgg acgagatcac ggccgacgag gcgacctgcg
      421 acggcaccga gacggtcgac ggcaagacct tgaaagtcta caagttcacg acgctggtca
      481 cgacaatgtt cggcgccggc acgacggtga cggcagtgtt tgtcggcgcg gacggcagac
      541 cggctcgaat tgattcggtg acgagcctgc cgaacatcct gaccaagcgc cggaacgtga
      601 tcacctacga tccggcgatc aagatcgagc cgccgaagat gtaacctcga aagcctcgca
      661 gccgcgcatg tccgagacgc cgaccatcga tgcgatccgc gccgatttcg ccctcctcga
      721 cgactgggag gaccgctatc gctatgtgat cgaactcggc cgggcactgc cgccgctgcc
      781 cgaggcactg cggactgaca ctaacaaggt gcgcggctgn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnccg tcgatgccca gaaggtgttc gccgagctcg gcctcaagga gcacctgacg
     1021 ccgcagcgct ccaacggcct cgcctcgatg gtggaacgta tccgcgtcga cgcgcggcgg
     1081 gcatgaccga cgtcgcgctg gcgctgctgc cgaccgtcgc cgtgctggtg gcgctgctcg
     1141 gattgcgctg gagcgggctc ggcgcggcgg gcctcgcggt ggcggtcgcg acggcgctgt
     1201 ttgcgctcgg tgcattcagg cccccgacgc accttgttgt cgcgcaggcc gtcccggatg
     1261 cgctgcttat cacgctgctc gtcgcatcca tggttgtgcc gggcgtactc ttcgtcattg
     1321 cggcttcgcg ccgccgggcg agtgagacgc tggccgggct cgttggcgcg attgatctgc
     1381 cgcccgcctc gcgtgtgatc gtcattgcgc ttgggatagg cgttgtggtc gagagcctga
     1441 cgggctctgg cgtatcgctg ctggtgacgg tgccgctact gctgcgattc accgaccgca
     1501 cccgttgcat gagcctcgcc ctcatcggca tgagcctgat gccctggggc gcgctcgcca
     1561 tctcggcgca tgtcgccggg gggctcgccg gcattccggt cgtcgagctg accacgccga
     1621 tctggtggct ctcgggctgc attgcgctca tacttccggt tctatgcctc gtggtggtgg
     1681 gagccaagca gacgagcccg cgggatgtcg tctttgccat cccggcgggg atcgtgctgg
     1741 ccggcacggc cgggcttgcc ggcaccggca tagggatcga gactgcgggc gtcctcggcg
     1801 gtctggcggt gctggcgctc ggcattgccg cttcgtccac acggcggggc cttggcgcgg
     1861 cgctcgccga gcccggcctg aggccgtatc tggctctgat cgttgttgtg ctgggccaaa
     1921 agctcgcgat cgcgacgctc gtgccggccc tcggcgtgaa ccccgtgctt tcgacgggtc
     1981 gtgtcacgtt ccgtctcctg acgtcgcccg gtatcccact tctcatcgtc actctgctcg
     2041 ccatggccgg atcgctggac ctggccgccg tacgacagat ggtcgagcgc tcgtggcggg
     2101 cgctcgcgac cgtgctgctc tttatgctct cggcccggct gatgctggag atcggtgcct
     2161 ttgcggccct ggcacggcag gtcgcgcagc ttggccagga catggccatc gttgcgacga
     2221 cgctcctcgg tgcggcgagc gggttcatta ccgggtcggg cgtctcggcg aacggcctgt
     2281 tcatgccggg ggcggcggca atcggccaag agttcggcct cacaagactt tttgcggcga
     2341 tccagaactg ctcggccggc cacgttgcca tggcatctct gccggtcggt gcgatcctgc
     2401 tcgcggcctt gcccgaccgg cagcgaggcg acgatcgcca ccttctcgtg accggccttt
     2461 ggctcgcggc actctacacg gctctgatcg ccgccgtcgc gatcggcgct gcgcggcttg
     2521 gcgtcggcgc ctgaagcata gaggccctaa cgcggcaccg gcgcggcggc tcgccgctct
     2581 aaaccaaaaa tttactcttt atgttcaatc gttaagcaat gccccgcaag ggttcggtcg
     2641 tattcggacc cgggacgtcc tggttgtcat ctcgagagag aatgcaccga cccttcgcgg
     2701 cgctgttgga tacgacggag cggtccggtg tttcgacgtg gcacaagcga tcaaccaaga
     2761 cgcccggcgc ggaaagcgct gccggcgcag ggttggcttg gcccgcgggc ccgcctactg
     2821 ctcgcggggt ttgccggggc ctcgctgttc gccgggctga ccggcggcgg ccgcgcgacc
     2881 cgcctcatgc cgccactctg gcaggagatc gaccggctcg ccgtccatgc cnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagacg agtgacaccg
     3001 agatttatgg cgtcctcgcc gatctcgcac gcggctcgct catcggcttc gatcctgtcg
     3061 ccgccaaggc gcgcatcgag gcgctgccct gggtcgagcg cgcgagcgtc ggccgcgaac
     3121 tccctgaccg gatcgtcatc gatatccgcg agcgcgtgcc ttatgccgtc tggcagaccg
     3181 gcgccgagct gatcgtcgtc gatgcgaccg ggcgcctgct cgccaaatcg gacgcggcat
     3241 cggctgccaa gctcggcttg ccgcgcatcg tcggtgacgg cgcgcccgag cgcgcccgcg
     3301 ccttccatgc cgaaattgcg gccttcccgc ggctggcgcg ccgtctcgcc gcagccgagt
     3361 tcgtcgcggg caggcgctgg acgctgcaca tgacgggcgg cctctctgtt ctgctgccct
     3421 cggcaggcat caaggaaacg ctcgccaggc tcgaccagct ccaggagcgc gaccagattc
     3481 tcgaccgcgc ggccgtgaca ctcgacctgc gcggcagcga tcggcttttc gtagaccccc
     3541 gacctgcctc cgcggcccca cgccgtctgc ccggatagcg cccatgctcg cgcgcgcccg
     3601 cccctcagga gaaggacgta tcgtcggctt gctggacatc ggggccggca aggtgtgctg
     3661 catgatcgcc tcgctctcgc cgcccgcgga aggcgcgaac tcagcgacgg tgcggctact
     3721 cggcttcggg caccagcgct cgcgcggcgt gaaagccggc gtcgtcgtcg atctcgagga
     3781 gacggtgcgc gcggttcaag ccgcggtcgg cgctgccgag cggatggccg gcctttcgat
     3841 cgacgatgtc tacctcgtcg tgatggcggg gcgtctcaag tcgtcgctgt tctcggccgc
     3901 ggttcaggtg gatcgcggcg tcgtcgagga cgagcacgtc gcgcgcgtgt tggcgggcgg
     3961 cgaggcctac ctcgcacgcg cggggcgggc catcatccaa cacgattgcg tgggcttccg
     4021 ccttgacggc tcgacgaacg tgcgtgatcc acgcggcctc gcaggcgcca agctcgtcgc
     4081 ggacctgcac gccgtgaccg ccgacgaggc gcccttgcgc aacctcgtgc tcgcggcgga
     4141 gcgctgccac ctgactgtcg tcggcatgct gtcagcaccc gctgcgagcg ggctcgcgac
     4201 ttcgaccgcg gacgagcgcg agctcggcgt cgtcgtcgtc gacatgggcg cgggtacgac
     4261 gtcgctcgcg gcctggagcc acggccgcct ccgtcatatc gatgtggcgc tcgtcggcgg
     4321 ccagcacgcg accttcgatc tcgccaacgc actcggcgcg ccgctcgatg aggcggagcg
     4381 gatcaagacg cggctcggca cgctcattgc ggcgcagtcg gacgagcgcg agcaggtctc
     4441 ctttccactg gccggcaacg atggcctggt cggtccgggc gaaggcatgg ccacacgagc
     4501 cgacgtgcgc cgcatcatcg cgccacgctt cacgcgcatc ctgcgcgatc tcggccagcg
     4561 catcgcccag catcccgagg ctcgagcggc ggcgtcccgt atcgtgctga cgggcggagc
     4621 gagccacttg ccgggccttc ccgaatgcgc ggccgacatc ctggacaagc ccgtccgcat
     4681 tggtcggccg atgtgtgcgg ggctaccggc gacggcctgc cagccgcagt tcgcggcgct
     4741 cattggcctg ctcgtcgccc ttgctgccac gccgtgcgac ggcgatccga tgctccgcgc
     4801 ctatcgagac cgaaatgtac tcacgggcgg ctacatcggc cgcctgaagg agtgggtgat
     4861 cggtaacttc tagcgtcccg atcgagactg aacgactgac cacggacaac gagacgaggc
     4921 tcacatgata aacctggcgt tgccacgggt cggcggaaaa ggcccgcgaa tcacggtcat
     4981 cggcatcggt ggcgccggcg gcaatgccgt caacaacatg atcgcctcgg gtctcggcgg
     5041 cgtcgagttc atcgcggcca atacggatgc gcaggcgctc gagacgtcga gtgcggaacg
     5101 gcgcatacag cttggccgca acctcaccga cggtctcggc gccggctcga agccggaagt
     5161 cggcgaggcg gcggccgagg agacgctcga cgagctgcgc gaggtgatcg agggatcgca
     5221 catggtattc ctggccgccg gcatgggtgg cggcacgggg accggcgccg cggccgtcgt
     5281 cgcgcgcacc gccaaggagc tcggcatcct gacggtcgcc atcgtgacga agccgttcgt
     5341 tttcgagggc gcgcgccgca tgcgcgctgc cgacgctggg atcgcggagc tgaagaagca
     5401 cgtcgacacg ctgatcgtga tcccgaacca gaaccttttc cgcatcgcca acgatcgcac
     5461 gaccttcgcg gaggccttca tcctggccga tcaggtgctc ttgtcgggca tcgcctgcat
     5521 cgtcgatctg atcgtcaagg aaggcctcat caacctcgat ttcgccgacg tgcagaccgt
     5581 catgtccggc atgggcacgg cgatgatggg aacgggcgag gcctcaggcc cgcggcgggc
     5641 cgtcgaggcg gcagaagagg cgatctcgaa cccgctgatc gacgatgtct cgctacaggg
     5701 tgcgcgcggc gtgctattgt cgatcatcgg cggccgcgac atgacgctct acgaggtgga
     5761 cgaggccgcg agccgggtcc gccaggaggt cgatccggat gccaacatca tcgtcggcgc
     5821 gacgttcgac gattcgctcg gggatcgcct cagggtggcc atcgtcgcct caggcatgca
     5881 tcaggccgag ctgcagccag gtgctgcccg catggccgag ccggcagggg cgccgcccat
     5941 gccacagccg atgccgacga acgccgccgg acagctcttt caggcacatg ccgaacaccc
     6001 ggcctttgcg cctcacgcgg tcccgaccgc tgacacttgg ccggccgagc cggcacccgg
     6061 gccgctgacg acacgcctgc gaaacctgcc gccgatccct gggggtccga tcgaggctca
     6121 ggtccgccca agctctgcca tcttcacgca cagtccagac gcgcgcccga ccatccactg
     6181 gcaggcgccc ggtgacgtga tgatcgagga tcggagtgcg caggatgccg atgtgccctc
     6241 gatgggcgat tggggtgcag cccatccgac ccggcccgca gacatcccgg cattcacgcc
     6301 gcagcccccg gcggaagcgc ggcgcacacc caccccgatc ccgccactcg accactttcc
     6361 ggcggtcggc cagcgcgagt atcgcgcgcg cgtcctggac gccgaaggct ccgagccgcc
     6421 aattcaggcg ccctcgcgcc atcaggctcc cacgcctgct gaaagcaggg gggggctgct
     6481 tcaacgactc gtcggtcgcg gacgctcccg cgaggatgcg cctcagaaga ctgaattgcc
     6541 tgggtttttt gagagccgcc gccggtgagt gcctgcgaca gccactgttg cagaacgtaa
     6601 cacatcgaaa gaaagcgtga tttgctggac acggagtcgc gcaataaggt caagatcaca
     6661 gcgaacgaat cacaccggtc accaacgcgc aaataactgc agcaagcatc agcgtggtgg
     6721 accaacaata atgtgatcgg ccgcttgggg ggtctaggta tctcagttgc gttgcacaac
     6781 agcgtcattg ggctcctggg ccaagggatc gagggacggc gctacatgtc gaagcgattc
     6841 atcggggcgc gacaaaccac agttggtcgc gagatcgaga tcaaaggcac cggcgttcat
     6901 agtggtgcgc ctgtctcgat cattctgcat ccggctccgg ccgacaccgg gatcacgttc
     6961 cttgccagca agcgaggcaa gatcgtctcg gagatccctg caaatgtaag tcacgtcaag
     7021 aacctgactt tgtgtacagt catcggcgac gatcgcggca cgacgatcgg cactgtcgag
     7081 catctgctgt ctgccctgcg cggcctctcg atcgacaatt gctacgtcca gatcgacagc
     7141 aaggaagtgc cgattatgga cggcagtgcc gcctacttcg tccaggcgat cgatgcggtc
     7201 ggcatccgcg agcttcagtc gccgcgcaag ttcatcaagg tgctgaagcc gatcctcgtc
     7261 gaggacaacg gcagtgtcgg cgagctgagc ccgtactcag gcttccatat cgactgcgaa
     7321 atccagttct cgagcccggt gatcggtcgg cagcgaatgg cgctcgacgt caaccccggc
     7381 accttccgca acgagatctc tcgcgcccgc accttcggct tcatgcacga cgtcgagaag
     7441 ctctggaagg ccggcctcgc actcggcgcc tcactcgaca atacggtcgc gatcggcgac
     7501 gaccgggtca tgaatcccga aggcctgcgc tacccgcagg agttcgttcg ccacaagatg
     7561 ctggatgccg tcggcgatct gacgctggcc ggcgcgccga tcctcggggc ctatcgttcc
     7621 gtgaagggcg gccaccggct caatgcacac gtgcttcagg ccctttttgc cgaccgcgac
     7681 gcctggacga tggtgcacgc accctgggta cgcgacggcg ttccggtcga catgccggca
     7741 ggcgcttcgg ccatgaactt cgcggccgat cattcttgag tggttcgcgg tcgggtgggc
     7801 tgtgcgtaat cctcgatggg cttcctggaa gtgcccttag ctggactgca ggataagcgc
     7861 tcgcagtctc attcgaaacc agaagactcc cgagccttcg aacttggtcg tgcatccaag
     7921 ctgtcagcag ttgtcgcgcg gcattgcgtc tcctgctgaa gacggcggcg atggaactgc
     7981 ggtacagagg cgatacagcc attggatccc tggccagtca gtgttgggct gacgagcgag
     8041 accaagcctc tcgcagaact gtcgaggagt agggtttcgc aggggttcgc gattgacgca
     8101 tcttggctgg cgagcgaagc ttcgcgtaag taggattgcg aatcggcgcc gcgtggggtg
     8161 gcgtggagca tgcggtgagg gcgcaccgcg ggacatgact ttcagcccgg tccagggcca
     8221 catggacaag ggtggagcga ctgaggatcg agtgcggttg gccaggcggt cagctcgcgg
     8281 ctcgatgaac aggcgagagc cactaacaaa agccacgaag atggcgacag gattggcgat
     8341 gaagctgcaa accgcattcc gccgcaaggc ctctctggca ctcgcctttg ccgcactctc
     8401 gggtgcgaca ctgcttgccg gctgctcgac atcgagcgac atggccaagg tgctcaatcc
     8461 cgatcctcca gcgaagatgt tcgcggatgc cgacgcggca ctcgcgcggg gcagctgggt
     8521 tgatgccgcg aagaagttcg aggatctgga tcgcgatcac ccctacgctc cggaagcgcg
     8581 gcgggccatc gtcatggcgg ccttcgccta ctacaaggcg gccaagttcc ccgacgccat
     8641 ctcggcggcg cgacgctaca ccacgatgca tccggggacg aaggaagcgg ccctcgccca
     8701 ccacatcatc gcctcggcgc acttcgacga gatccaggtg ccctcgcgcg accagacgaa
     8761 tacgcgcaag gcgctcgccg agctgaagac gctccgggca cgctatcccg acagcccata
     8821 tgccaagcag gctgagaacc gcatccggat cgccgaggat gtgcttgccg cgtccgagat
     8881 gaacgtcggt cgctactacc agaagaaccg gaactatgtg gcggcgatca accgattccg
     8941 cacggtcgtc gtcgagtacc agacgacgac ccacgtcgag gaggcgcttt tccgattgac
     9001 cgagacctac atgttccttg gcatccgcaa cgaggcacag acggcggcgg ccgtgctcgg
     9061 ccacgcggct gacgaggtag ttcggcaggt ccttttgcca gcccatgtcg gggaaggtgc
     9121 cgtggagcga ggcgagaacc tcggcctcgg cgccgtaacg ccgtgcgccg agtaggcact
     9181 cgaccgttag cgcctcgagt cccttgatca tcacgctgcg gcacatcttg atcgccgacg
     9241 cgacgccgat cgtatcggcc acggcggtag catcgaagcc
//
LOCUS       DAORNH010000181         9274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5513_length_9274_cov_2.750081, whole genome shotgun sequence.
ACCESSION   DAORNH010000181 DAORNH010000000
VERSION     DAORNH010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5513_length_9274_cov_2.750081"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..605)
                     /locus_tag="PK264_16675"
     CDS             complement(168..605)
                     /locus_tag="PK264_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD77542.1"
                     /translation="MQSELVPVDEVPVEATFAALSANRRAVLIDVRTRSEWTFVGVPD
                     LSAIGKRAILVEWQTFPDQRGKSDFPERLAAELDKTGVTRDDDLYFLCRSGGRSLSAA
                     KAMAAVGYRRCHNASEGFEGPLGPDRKRNLAGWKVSGLPWSQS"
     gene            complement(1950..2213)
                     /gene="rpsT"
                     /locus_tag="PK264_16680"
     CDS             complement(1950..2213)
                     /gene="rpsT"
                     /locus_tag="PK264_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRD77543.1"
                     /translation="MANTSSAKKAARKAIRRTEINKGRSTKLKTAVRKVEAAIASGNK
                     EAAAAELKAVEPVLMRSAQIGVTHKRTSSRKVSRLTARVRAMA"
     gene            2497..3210
                     /locus_tag="PK264_16685"
     CDS             2497..3210
                     /locus_tag="PK264_16685"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRD77544.1"
                     /translation="MNERHDLAGTILGIFCPVHRDGYKFIAITAIPAAILLVLWWPIG
                     IVFAGLACYVAYFFRDPARVTPMRDGLVIAPADGRVSSLDRVRPPAELGLGDEPRQRI
                     SIFLSVFDVHINRSPIAGRISRSVYVPGAFLNAALDKASEENERRAIVITPTSGEPIA
                     VVQIAGLIARRIVTFKGEGEQVGAGERFGLIRFGSRVDVFLPSGRHALVSIGQRAVGG
                     ETVLADLRSDEPERVARVG"
     gene            complement(3215..3703)
                     /locus_tag="PK264_16690"
     CDS             complement(3215..3703)
                     /locus_tag="PK264_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HRD77545.1"
                     /translation="MSEGPPPLESLDGRQSAAAMEIRRGAQRLLRAHGLASLPEVTLA
                     SGRRADLLALAPDGRIAIVEIKSSVADFRADHKWPDYQAFADQLYFAVGPAFPQALIP
                     ESAGLIVADRYGGEVVREAPTCKLAPARRKAVTLLLARIAASRLHAGADPEAFQGEIA
                     DG"
     gene            complement(3700..4482)
                     /locus_tag="PK264_16695"
     CDS             complement(3700..4482)
                     /locus_tag="PK264_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD77546.1"
                     /translation="MSKNEVLTRQDGAVLTITLNRPEKFNALTFSMYQQLHDIVTGLP
                     TDGRVRAIIVTGSGEKAFAAGTDISEFRSFSSPADGIGYETRVDQILTAIEACPVPLI
                     AAISGACTGGGAAIAACSDIRIATRSLKYGFPIARTLGNCLSAASLARLVALVGQPKV
                     VDLVFSSRLMQADEAKAAGLVSELHEDHASLMARANALAAEIAGYAPLTLSTTKALLR
                     RMRQAMPKVDDHDLVAKVYTSADFREGLEAFLAKRPPRWSGK"
     gene            4685..5719
                     /gene="dusB"
                     /locus_tag="PK264_16700"
     CDS             4685..5719
                     /gene="dusB"
                     /locus_tag="PK264_16700"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRD77547.1"
                     /translation="MTETTGATHHTADALSVGPVALANRVALAPMSGVSDLPFRRLAQ
                     SFGAGMVVSEMVASRELVSERRDVLRRAEGRDLAPFVIQLVGCEANWMAEGARRAEAL
                     GADIIDINMGCPSREVTGRLSGSALMREPDHALSLIEAVVGAVEVPVTLKMRLGWDDR
                     SINAPEIARRAEAAGVKLLTVHGRTRCQFFKGVADWSRVATVCEAVDIPVLVNGDITT
                     PEDAEKALAASGANGVMIGRGAYGAPWAPARIATTLATGSDPGPPSLATQVATARAHI
                     EAMLSHYGIALGLRNARKHVGWYIETSGAGPELARATRRKLCTEESAPALLRGLTAFY
                     ETLESEPVAA"
     gene            5716..6861
                     /locus_tag="PK264_16705"
     CDS             5716..6861
                     /locus_tag="PK264_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77548.1"
                     /translation="MSAQSIEQITQAAGQRRHVEHDVLLMALPHPIMVLGEEDRVVYA
                     NASTESFLQTSLASLRRLTLPEIVAFGCPLVALIAQVRRSGQSMNEYGVELATPKVPV
                     PRLVDIYAAPLAEAPGHIIVMLQQRSMAQMIERQLTHRAAARSVSGMAAVLAHEIKNP
                     LSGIRGAAQLLEPGLSDEDRALTQLICQETDRIRDLVDRMEVFGDERPLAKEPVNIHA
                     VLDHVRRIAESGFAAGIRFVETYDPSLPPVPASRDKLIQAFLNLVKNAAESIREAREP
                     VGRIQISTAFRPGVRLAVPGSDARVSLPLRIEIEDNGTGISEAIKPHLFDPFVTSKPT
                     GTGLGLALVAKIIRDHGGIIEFESEPRRTVFRVLLPLADKPARDRKE"
     gene            6866..8317
                     /gene="ntrC"
                     /locus_tag="PK264_16710"
     CDS             6866..8317
                     /gene="ntrC"
                     /locus_tag="PK264_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HRD77549.1"
                     /translation="MSRGTVLIADDDGAIRTVVSQALARAGFVPRATGNAATLWRWVA
                     QGEGDVVITDVILPGENGFDLLPRIRKQRPDLPIIVMSAQNTLMTAIKAAEKGAYEYL
                     PKPFDLAQLVTFVGNALSEPGRRRPVEQSAGEEEMPLIGRSAAMQEIYRVLARLTQTD
                     LTVMITGESGTGKELVARVLHKFGKRSAGPFVALNMAAIPRELIESELFGHEKGAFTG
                     AQARTSGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQEGEYTTVGGRTSIKTDVRIV
                     AATHRDLKNQIQQGMFREDLYYRLNVVPLRLPPLRERPDDIGDLVRHFLRQAAREGLP
                     AKTIETAAIERLKRFAWPGNVRELENLVRRLVALHSDETITVDRVETELQSGVRAEPV
                     LTEGDAASSLSEIVERHLATLFAEAGRDLPAPGLYRRIIRQVELPLVQAALAATRGNQ
                     IRAAELLGLNRNTLREKIRDLDIQVFRAPGSNS"
     gene            8418..8711
                     /locus_tag="PK264_16715"
     CDS             8418..8711
                     /locus_tag="PK264_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012178707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRD77550.1"
                     /translation="MSSNIPQFLVHSPKDNVGVVVVEDLKAGTDMLGVITETDTTIRV
                     TAKHDIPIGHKVALTELKTGDTVIKYGEDVGRMIGPATKGGHVHVHNHKTKRW"
     gene            8724..>9274
                     /locus_tag="PK264_16720"
     CDS             8724..>9274
                     /locus_tag="PK264_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRD77551.1"
                     /translation="MAAKKSGKATGSANLSFRGWRRENKRVGVRNHVLILPLDDLSNA
                     ACEAVANNIKGTLAIPHAYGRLQFGADLDLHFRTLIGTGSNPNVAAVVVIGIEDGWTD
                     RVVKGIAKTGKPVVGFGIEGHGDIATIAKASYKAKEFVHWASELKREECPISDLWVST
                     KCGESDTTTGLSSCPTVGNMYDKL"
BASE COUNT         1557 a         3176 c         2956 g         1585 t
ORIGIN      
        1 atgcgtcgcc accccggtag tctgtccggt cgtcacccat ttgttacccc gcacgtctgt
       61 ttgttgccct ctgcaccgcg caacaacctg tccacgcccg ccgccacggg cgcgaggcac
      121 aagtcgattg cgagtgctta ttcttgtcgt tcgtccgtcg agcgcgccta gctctgagac
      181 cagggcagtc cgctcacttt ccagccggcc aagttgcgct tgcgatcggg tccgaggggg
      241 ccctcgaagc cttcgctcgc gttatggcag cgcctgtagc cgacagcagc catcgctttg
      301 gctgcgctca ggctccgccc acccgacctg cacaaaaagt acagatcgtc gtctctcgtc
      361 acaccggtct tgtcgagctc cgctgcaagc cgttccggaa agtcgctctt gccgcgctga
      421 tccgggaacg tctgccactc gacgagtatc gctcgctttc cgatcgccga cagatccggc
      481 accccgacga acgtccattc cgatcgcgtc cgcacatcga tcaggactgc acgcctgttc
      541 gcggacaagg cggcgaatgt ggcctcgacc ggaacctcgt caactggaac caactccgac
      601 tgcatcaacg aactactcca cgtttcggtg tgggcccctc gatcagcgtc cgacagcgcc
      661 gctacctgcc gcccgccgcg tcacgaaaag ctctcagtcc tgaaccgtgt cgcctgccat
      721 atgtcaggca cgcacggctc cgtccgcttc aatttcctct cggcctggac ccgctctccg
      781 cttgggtcct acagcccgtc accgacaggc ccatctctcg agattgtcga taccaagatc
      841 gctacggcag tgaaagtcga tgcggagaaa gtctatcgat gcgtcacagg catccttgcc
      901 gtaacggcct cgctgcactc tgcagatttc gctgcactgt gcagtcgctg gacgaagtgc
      961 agtgagcgcc aatccgaacg gcgtcccatc acggacccgg aacgccggcg ttcgacgttt
     1021 cgatccagca cgacgattcc gcacgaaaaa cgcgagcgtg gccgtcgccg cctgtcccga
     1081 tcgtgagcac aacttgctcg cggacgctcg gaactcattc gagggaagat cccgcaaagc
     1141 gccactctgc cgcgccggaa ccttcggatt ttcctgcggt tcggcgttgg accgccctaa
     1201 ccgtcatggg ggagtctata ggccgaccca gaggccgaag gcaagtccac aaagggctcg
     1261 gatcgcgcca tccgaccgtg agtccggccc gccaatctcc actggatctc cacatcccac
     1321 gcccccgcct gtggagaaat taaccatttg cgcccgggtg ttcgggggct cgcctgagtg
     1381 cgccgcatca attcatccag aggcaaaatt ttcgtggaat aaaatctgtt ttcacttgat
     1441 cgccatgcgc tggctgcaag ccttgagacg agcaatgtag ccggatgcac gggtaaccca
     1501 ttatgtattc tgaggttcac gagcataagg cggcgctggc agttcgagtc tctctggcgg
     1561 cccgtggcga ggcgaccaca cgccggctct ggacgagcgg gctttgccat catgacacgc
     1621 tcgcgacttg gggctcgcgg ggtggccata gcgcacgaaa tctcggtgca ggttcgcaat
     1681 tcaggcgatt gtgacgcgcc tcctcattca cagaagacgc tcgtgatcat atttcatcca
     1741 caggccaccc cggttttagt ggccaacccg cctgaagggc gacctcaagt aggtcgcgaa
     1801 ggccgcgact tcagtcgatt ctaatgcgcc gatgccttgg ccggctatcg gccagggggc
     1861 gtccgtcagg cggcattcag ccggtcgaca cagaatggcc ccggtcgctc gaccggggcc
     1921 gcaaagctgt cggacgctgg gaaggtcgtt caggccatgg cgcggacgcg agccgttagg
     1981 cgcgacacct tccgagagga agtgcgcttg tgagtgacgc caatctgcgc cgatcgcatg
     2041 aggaccggct cgacggcctt gagctcggcg gcggcggcct ccttgttgcc cgaggcgatg
     2101 gccgcctcga ccttgcggac ggccgtcttg agcttcgtgc tgcgaccctt gttgatctcg
     2161 gtgcgacgga tcgccttgcg cgcagccttt ttcgccgagc tggtgtttgc catgaacctc
     2221 tgcctcttgc gttgtcgcgc gccgcggcgc gcgatcgctt tgcgatgagt tggaattcga
     2281 gtgtgtcagc acgcatggcg cccggggcgg caccccgggc ggctcgtgtc ccggatcctc
     2341 ttagccgtga gggctcccgc ggtcaatcgc tctcctctgc cgggcagccg aggaatgcgt
     2401 gccgactgca ttgcgtgcca tggcgacttg gcgtagctca acgcccgcac gtccttcgtc
     2461 ggcgtgccca tgtcgtgcca gcggaggatg ccccacgtga acgagcgcca tgaccttgcc
     2521 ggcacgatcc tcggcatttt ttgtcccgtc caccgcgacg gctacaagtt catcgccatc
     2581 accgccatcc cggccgcaat ccttctggtg ctgtggtggc cgatcggtat cgtcttcgcc
     2641 gggctcgcct gttatgtcgc ctacttcttt cgcgatccgg cccgcgtcac gccgatgcgc
     2701 gacggcctcg tcatcgcccc ggcggatggc cgtgtgagtt cgcttgatcg ggtccgcccg
     2761 ccggccgagc tcggtctcgg cgacgagccg cgccagcgca tctcgatctt cctctccgtc
     2821 ttcgacgtgc atatcaaccg ctcgccgatc gcggggcgca tctcgcgctc agtctatgtg
     2881 ccgggcgcct ttctcaatgc cgccctcgac aaggcgagcg aggagaacga gcgccgtgcc
     2941 atcgtcatca cgccgacgag cggcgagcca attgccgtcg tgcagatcgc cggcctcatc
     3001 gcgcgtcgca tcgtcacctt caaaggcgag ggcgagcagg tcggagccgg cgagcgcttc
     3061 ggtctcatcc gcttcggcag ccgtgtcgat gtcttcctgc cgtcgggccg gcatgcgctc
     3121 gtctcaatcg gccagcgcgc cgtcggcggc gagacggtgc tcgccgatct gcgctcggac
     3181 gagcccgagc gggtcgcccg cgtcggttga cacgctatcc gtcggcgatc tcgccttgga
     3241 aggcctcggg gtcggcgccg gcgtgcagcc ggctcgccgc gatacgcgca aggagcagcg
     3301 tcacggcctt gcgccgggcg ggcgcaagct tgcaggtcgg cgcctcgcgc acgacctcgc
     3361 ctccatagcg atccgcgacg atgaggccgg cgctctccgg gatgagtgcc tgtggaaacg
     3421 cgggcccgac ggcgaaatag agctggtccg cgaacgcttg atagtccggc catttatggt
     3481 cggcgcggaa gtcggcgacg ctcgacttga tctcgacgat ggcgatgcgg ccgtccggag
     3541 cgagggcaag gagatcggcg cgccggccgc tcgcgagcgt tacctcggga aggctcgcca
     3601 gaccgtgagc gcgcagcagc cgttgcgccc cacgcctaat ctccatggcg gctgcggact
     3661 gccgtccgtc gaggctttcg agtggcggcg ggccttcgct cacttgccgc tccagcgtgg
     3721 cgggcgcttg gcgagaaacg cctcgagccc ctcgcggaag tccgcgctcg tgtagacttt
     3781 ggcaacgagg tcgtggtcgt cgaccttcgg catcgcctgg cgcatacggc ggagcaaggc
     3841 cttggtcgtc gagagtgtca gcggcgcata gccggcgatc tcggccgcga gcgcgttggc
     3901 ccgtgccatc aggctggcgt gatcctcgtg gagttcgctc acgagtccgg ccgccttggc
     3961 ttcgtcggcc tgcatcagac gcgaggagaa gacgagatcg acgaccttag gctggccgac
     4021 gagggcgacg agccgggcga ggctcgcagc ggacaggcag ttgccgagcg tgcgagcgat
     4081 cggaaagccg tacttgagcg accttgtggc gatacggatg tcggagcagg cagcgatcgc
     4141 cgcgccgccg ccggtgcagg cgccggagat cgccgcaatc agcggtaccg ggcaggcctc
     4201 gatggcggtc aggatctgat cgacgcgcgt ctcgtagccg atgccgtcgg cggggctcga
     4261 gaacgagcgg aactccgaga tgtcggtgcc ggccgcaaac gccttttcgc cggagccggt
     4321 cacgataatg gcgcgcaccc tgccgtcggt cggtagcccc gtcacgatgt cgtggagctg
     4381 ctggtacatc gagaaggtca gcgcgttgaa cttctcgggc cggttcaagg tgatggtcag
     4441 tacagcgccg tcttggcgcg tcaatacctc gttcttcgac atgggtgtgc tcctggatct
     4501 cggttgcgtc accatagcgg ccgattggcg acgccgggag gggctgagcg ctcgaggtca
     4561 attgttcgca cttgcaaaat cagaggcgag ccactaagtt gcacattaat tagacatcaa
     4621 gtgtcgacgc cttaaaaata ggcaaatgct aaatgctcgt gcagccgatc caggctatgg
     4681 ggtcatgacc gagacgaccg gagcaacgca ccacactgcc gacgccttga gcgtcggccc
     4741 ggtcgcgctc gccaaccgag tggcgctggc gcccatgtcc ggcgtctccg acctgccatt
     4801 tcgccgcctt gcccagagct tcggcgccgg catggtcgtc tccgagatgg tggcgagccg
     4861 cgagctcgtc tccgagcgcc gcgacgtgct gcgccgtgcc gagggacgcg accttgcgcc
     4921 gttcgtcatc cagctcgtcg gctgcgaggc gaactggatg gccgagggtg cgcggcgcgc
     4981 cgaggcgctc ggtgccgaca tcatcgacat caatatgggc tgcccctcgc gcgaggtgac
     5041 cggacgcctc tcgggtagtg cgctgatgcg cgagcccgac cacgccttgt cgctgatcga
     5101 ggctgtggtc ggcgccgtcg aggttccggt gacactcaag atgcgactcg gctgggacga
     5161 caggagcatc aacgcgcccg aaatcgcgcg ccgagccgag gcggccggcg tcaagctcct
     5221 cacggtgcac gggcgcacgc gctgccagtt cttcaagggc gtggccgact ggtcgcgcgt
     5281 tgccacggta tgcgaggcgg tcgatattcc cgtgctcgtc aatggcgaca tcaccacgcc
     5341 cgaggatgcg gagaaggccc tcgccgcttc aggcgccaac ggggtcatga tcggccgtgg
     5401 cgcctatggt gcaccctggg cgcccgcgcg cattgcaacg acactggcga cgggctccga
     5461 tcccggtccg ccgagccttg cgacacaggt cgcgaccgcg cgcgcccaca tcgaggcgat
     5521 gctctcccac tacggcatcg cgctcggcct caggaacgcg cgaaagcacg tcggctggta
     5581 catcgagacc agcggtgccg gcccagaact ggcccgcgcc acccgacgca aactctgcac
     5641 cgaggagagt gcccctgcgc tgctccgcgg cctcaccgcc ttctacgaaa ccctcgagag
     5701 cgagcctgtc gcggcatgag cgcccagagc atcgagcaga tcacgcaagc ggccggtcag
     5761 cgccgtcacg tcgagcacga tgtgctgctg atggcgctgc cgcatccaat catggtgctc
     5821 ggcgaggagg accgcgtcgt ctacgccaac gcctcgacag agagtttcct gcagacgagc
     5881 ctcgcctcgc tccgccgcct gacgctgccc gagatcgtcg ccttcggctg cccgcttgtt
     5941 gccctcatcg cgcaggtgcg ccgttccggc cagagcatga atgagtacgg cgtcgagctc
     6001 gcgacgccga aggtgccagt gccacgcctc gtcgacatct acgcggcacc ccttgccgaa
     6061 gcgcccggcc atatcatcgt catgctgcag cagcgttcca tggcgcagat gatcgagcgg
     6121 caattgacgc accgcgccgc cgcgcgctcc gtctcgggta tggcggcagt cctcgcccac
     6181 gagatcaaga acccgctctc cggaatccgc ggggccgcgc aactcctcga gccgggcctc
     6241 agcgacgagg atcgggcgct cacccagctc atctgccagg agacggaccg tatccgcgac
     6301 ctcgtcgacc gcatggaggt gttcggcgac gagcgcccgc tcgccaagga gccggtcaac
     6361 attcatgctg tcctcgatca cgtgcgccgg atcgccgaat cgggctttgc cgccggcatc
     6421 cgcttcgtcg agacctacga tccttcgctg ccccccgtgc cggcgagccg cgacaagctc
     6481 atccaggcct tcctcaacct cgtgaagaac gcggccgaat cgatccgcga ggcgcgcgag
     6541 ccggtcgggc gcatccagat ctcgacggcc ttccgccccg gcgtgcgcct cgccgtaccg
     6601 gggagcgatg cccgcgtctc gctgccgctc cggatcgaga tcgaggacaa cgggacggga
     6661 atctccgagg ccatcaagcc gcacctcttc gatccgttcg tcacctcgaa gccgaccggg
     6721 accggcctcg gcctcgcgct cgtcgccaag atcatccgcg accatggcgg catcatcgag
     6781 ttcgagtcgg agccccgacg caccgtgttc cgcgtcctgc tgccgctcgc cgacaagccg
     6841 gcgcgcgatc gaaaggagtg agcccatgag ccgcggcacc gtactcattg ccgacgatga
     6901 cggcgccatc cgcaccgtcg tcagtcaggc gctggcccgg gcgggcttcg tgccgcgcgc
     6961 gaccggcaac gcggcgacgc tctggcgctg ggtggcgcag ggcgagggcg atgtcgtcat
     7021 cacggacgtg atcctgcccg gcgagaacgg cttcgacctg ctgccgcgca tcaggaagca
     7081 gcgccccgac ctcccgatca tcgtcatgag cgcgcagaac acgctgatga cggccatcaa
     7141 ggccgccgag aagggcgcct atgagtacct gccgaagccg ttcgacctcg cccagctcgt
     7201 caccttcgtg ggcaacgcgc tgtccgagcc cgggcgccgg cggcccgtcg agcagtcggc
     7261 aggcgaggag gagatgccgc tcatcggccg ctcggcggcg atgcaggaga tctaccgcgt
     7321 actcgcgcgc ctgacgcaga cggacctcac ggtgatgatc accggtgaat ccggcacggg
     7381 aaaggaactc gtcgcccgcg tcctccacaa gtttgggaag cgcagtgccg gccccttcgt
     7441 cgccctcaac atggcggcaa tcccgcgcga gctcatcgag agcgaactct tcggtcacga
     7501 gaagggggcc tttactggcg ctcaggcccg cacctccggc cgcttcgagc aggcggaggg
     7561 tggcacgctc ttccttgacg agatcggcga catgccgatg gaggcacaga cgcggctcct
     7621 gcgggtcctg caggagggcg agtacacgac cgtcggcggg cgcacctcga tcaagacgga
     7681 cgtgcgcatc gtcgccgcga cccaccgtga tctcaagaac cagatccagc agggcatgtt
     7741 ccgcgaggac ctctactacc gcctgaacgt cgtgccgctt cgcctgccgc cgctgcgcga
     7801 gcgccccgac gatatcggcg acctcgtccg ccattttctc cgccaggcgg cccgcgaagg
     7861 cctgcctgcg aagaccatcg agacagcggc cattgagcgg ctgaagcgct tcgcatggcc
     7921 cggcaacgtg cgcgagctcg aaaacctcgt ccgccgcctc gtcgcacttc attccgacga
     7981 gacgataacg gtcgaccgtg tcgagacaga gctgcagtcg ggcgtgcgcg cagagccggt
     8041 gctcacggaa ggcgacgcgg cgtcgagcct ctccgaaatc gtcgagcgtc atctcgcaac
     8101 gctcttcgcc gaggcgggcc gcgatctgcc cgcgcccggg ctttaccggc gtatcatccg
     8161 ccaggtcgag ctgccgctcg tccaggcggc gctcgcggcg acgcgtggca accagatccg
     8221 cgcggccgag ctcctgggcc tcaatcgcaa cacgctccga gaaaagatcc gcgatctcga
     8281 catccaggtg ttccgcgcgc ccggctctaa cagctgagcc gcggcgctcg aggcggttta
     8341 ccctcagcct gctattccat ataactgtcg ctgctcatcg tgttccatcc gccatcgcga
     8401 acggagagag aggcagcgtg tcatcgaata tcccgcagtt cctggtgcac agtcccaagg
     8461 acaacgtggg cgtggtcgtg gtcgaggatc tgaaggcggg caccgacatg ctcggcgtca
     8521 tcaccgagac cgacaccacg atccgcgtca ccgccaagca tgacatcccg atcggccaca
     8581 aggtggccct cacggaactg aagacgggcg ataccgtcat caagtacggc gaggatgtcg
     8641 gccgcatgat cggccccgct accaagggcg ggcatgtcca cgtgcacaat cacaaaacga
     8701 agcgctggta gggcggggcg aaaatggcgg ccaagaaatc cggcaaggcg accggttcag
     8761 caaacctctc cttccgcggc tggcggcgcg agaacaagcg cgtcggcgtg cgcaaccacg
     8821 tgctcattct gccgctcgac gatctgtcga atgcggcgtg tgaagccgtt gccaacaaca
     8881 tcaagggtac gcttgcaatc ccgcatgcct acggccggct gcagttcggc gccgacctcg
     8941 acctgcattt tcgcacgctg atcggcactg gctccaaccc gaatgtcgcc gccgtcgtgg
     9001 tgatcggcat cgaggacggc tggacggacc gcgtcgtcaa gggcatcgcc aagaccggga
     9061 agcccgtcgt cggcttcggc atcgaggggc acggcgacat cgcgacgatt gccaaagcct
     9121 cctacaaggc gaaggagttc gtgcactggg cctcggagtt gaagcgcgag gaatgcccga
     9181 tctccgatct ctgggtctcg acgaagtgcg gcgagagcga cacgacgacc ggcctctcct
     9241 cctgccccac cgttggcaac atgtacgaca agct
//
LOCUS       DAORNH010000182         9204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5565_length_9204_cov_2.655481, whole genome shotgun sequence.
ACCESSION   DAORNH010000182 DAORNH010000000
VERSION     DAORNH010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5565_length_9204_cov_2.655481"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..489
                     /locus_tag="PK264_16725"
     CDS             85..489
                     /locus_tag="PK264_16725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD77552.1"
                     /translation="MFETSLPLRFAHCDPAGIIFYPQYFVLFHELMEDWFTKGLGVNY
                     ADMIYRERIGVPAGRVECEFLAPSRIGETLTFSLRLARIGSSSLTMKIDCRKGSEARA
                     RAAITVILAQLEGPKALPIPDDLRRKMLDYLE"
     gene            complement(496..2502)
                     /locus_tag="PK264_16730"
     CDS             complement(496..2502)
                     /locus_tag="PK264_16730"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HRD77553.1"
                     /translation="MQVLGTALAATRQYLAPLIDIARATVLRPSALMGLMLLAATAAV
                     LLPISAVQTLRERTFDAFLAARAPPQPTPPVIVVDLDRATLTAGGGNSISREQLALIV
                     DRIAEAAPAVLAIDMLIEGPDDRSPAALARRLADVTKDAGVAALVDKLPDADTRLAAA
                     LRKAPTAVGFVLDPDGAGTLLPPAPILVRGTPDVRDIWTAEGAIGPTEIIAEAAQGFG
                     ALVMAADPDGRIRSVPLILRAGNLLRPGLALEAIRLASGTGSYVLESSPFSARLGPRL
                     FAFSRDASLRLRPTSQTRHAGRTLSAQAVLADAAARARIKGAIVFLGSSAPELAGLRT
                     TAGGNLVASVQLHADAASQLLAGDLPYRPAIAGWLEAAMMLGFGGLLVRAGLCWSAMK
                     AAALAIGGGLVWLVLASAGIAFGGWLIDPLAPPVVAGLGYGVAATIAAAHTRRREAAV
                     RRSFEQHLAPEVVRRIVESPGLLRLEGEMREITALFTDIEGFTSMTERSDPRALVRVL
                     DGYIEGASRIVVEHGGMVEKIVGDGLHAIFNAPLDLTNHPQKGLACAEALLAFSEAYR
                     REAEPAALMLGRTRLGLETGLVVVGDVGGGRRLDYTAHGNAMNLAARLEAANKELGTS
                     ICIGPEAASRIGVERLTSLGGLAVRGRGEVLEVFTVGPRPAKRS"
     gene            complement(2692..3351)
                     /locus_tag="PK264_16735"
     CDS             complement(2692..3351)
                     /locus_tag="PK264_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD77554.1"
                     /translation="MALALYGHPFSSYTQKVLIALYESDTAFEFRCIGPDTPDHVRDW
                     SRRWPMGKFPLLVDGDASIAESSIIIEHLDIHHPGKARLIPADPNRALAVRFLDRYFD
                     FHVMDAMQIAVEAALGRLPIKREDGIAVAAGRLKRAYAWLETELAGRPWATGDAFTMA
                     DCAAAPSLFYADWTHPIGHDFPVLRAYRARLLQRPSFARAVDEARPFRSLFPLGAPSR
                     D"
     gene            3520..4158
                     /locus_tag="PK264_16740"
     CDS             3520..4158
                     /locus_tag="PK264_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRD77555.1"
                     /translation="MIYELRIYTVKAGTVGEVAKNSGTVARSIRGDDYGKLEGYWITE
                     IGPLDQVVHLWSYKDLNERQRLRTELGKNVRWTNEYLPLIRPHMLRQDIRLLNHVIGV
                     NPPAKQGSNVYELRYYRTRPGGVKEWVGHFSEALPVREKHSKIVGLWTGEAGQPNEVV
                     HIWAYADLNARAAARNAAIQDPGWQAFLKKATPLLDEMNSVIMLPAAHSPLK"
     gene            complement(4192..4995)
                     /locus_tag="PK264_16745"
     CDS             complement(4192..4995)
                     /locus_tag="PK264_16745"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HRD77556.1"
                     /translation="MAHTPPVAAHATGPSAPLVLLVADGRMGAIVANAFADRFDRLVV
                     IVEEPERKSVVIQRRARLLGWPTALGQVANGLLTRVAGRLNRRRFAELDRTYGFRTDF
                     GKGLRIELVSSVNSEACRDLLRRLRPSVVAVYGTRIISRATLSAIEAPFINYHAGINP
                     KYRGQDPAYWALASGDAEHAGITIHLVDQGVDTGKVIAQAPVRFDPRDTIATYQYAQL
                     GVALPVFMHAIGEARSGRVATTTVDLPSRQWFPPTIWRYLWNGLRRGVW"
     gene            complement(5150..>5364)
                     /locus_tag="PK264_16750"
     CDS             complement(5150..>5364)
                     /locus_tag="PK264_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD77557.1"
                     /translation="FLDRETLAGRHPGISLDGLPAEGAVTVVLRVGDDEAAAAALAGR
                     KTTTEPGVVKVHPSDANGVILVLTTG"
     assembly_gap    5365..5378
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5379..6006)
                     /locus_tag="PK264_16755"
     CDS             complement(<5379..6006)
                     /locus_tag="PK264_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD77558.1"
                     /translation="MSLANVAGLDHIVIVVRDLAAAAENWRRLGFKVSDRGLHSAHMG
                     TANHTIMLDEDYIELIGVVAETERNVPTRAFLDRRGEGIERAAFTARSAAGLAKELAA
                     NGFPPIGPVAFSRPVERADGVKSDARFETTQWPVDQRPGGLRIFACQHFTRENVWLPE
                     LLGHANTARRIERLELLSTAPRAAADHMSRMIDMPIEAEPDGAFRVASG"
     gene            6197..6364
                     /gene="rpmG"
                     /locus_tag="PK264_16760"
     CDS             6197..6364
                     /gene="rpmG"
                     /locus_tag="PK264_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRD77559.1"
                     /translation="MAKPITQKIKLLSTAGTGFFYVTKKNPRTSTDKFVFKKYDPIAR
                     KHVEFKETKIK"
     gene            complement(7179..8546)
                     /locus_tag="PK264_16765"
     CDS             complement(7179..8546)
                     /locus_tag="PK264_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HRD77560.1"
                     /translation="MTARVLVVDDILANVKLLEARLSAEYFEVLTAFNAKDAIEICSR
                     ERVDVVLLDVMMPGMNGFEACRIIKSNPRTAHLPIVMVTALDQPSDKLQGLESGADDF
                     LTKPVDDIALITRVKNLARLKVLSDELIMRASTSEQMGLPSIDQLLAGGSDKGRILLV
                     EDHPRSGQRLTEALGKVHEIALEKDPQAALLRAAEENFDLLVVSLSLAESDGLRLCSQ
                     VRSLERTRHLPIIILVEPGDDARLLRGLDMGVNDYLIRPIDRNEMLARVRSQVRRKRH
                     QDLLSTRLKQSVELAITDSLTGLHNRRYMEGHLKTLVADAIAGNRHLSVLVADIDYFK
                     SVNDTYGHDAGDTVLVEFAQRFRRNTRGIDLACRLGGEEFVIIMPDTDLAVARQVGER
                     LRACIAADPFQINSSTQLRVTASVGLATLERRDDTPMALLKRADLALYAAKREGRNRV
                     VTAAA"
     gene            complement(8546..9010)
                     /locus_tag="PK264_16770"
     CDS             complement(8546..9010)
                     /locus_tag="PK264_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77561.1"
                     /translation="MTYQETSNSIQARRPPGGKKLVLIVEDNELNMKLFHDLLDANGF
                     ATLRTGNGIEALALARSHRPDLIIMDIQLPEVSGLEVTRWIKEDDALRTIPVIAVTAF
                     AMKGDEERIRRGGCEGYLSKPISISRFIEAVQRFIGEGRPAASGRTQTMGHS"
BASE COUNT         1633 a         2881 c         3133 g         1543 t
ORIGIN      
        1 gcgcgcgctg cgcatctatg aaggcgcgtc ggaagtgcag aaggtcgtga tcgcgcgtca
       61 gcacctcgcc cagagcgcgc cctgatgttc gaaaccagcc tgccgctgcg cttcgcgcac
      121 tgcgatccgg cgggcatcat cttctacccg cagtacttcg tactcttcca tgagctgatg
      181 gaggactggt tcacaaaagg cctcggcgtg aactacgccg acatgatcta ccgcgagcgc
      241 atcggcgtgc ccgctgggcg tgtcgagtgc gagttcctgg ccccgagccg tatcggcgag
      301 acactcacgt tctcgctccg cctcgctcgc atcggctcga gctcgctcac catgaagatc
      361 gattgtcgca agggcagcga ggctcgcgcg cgagccgcga tcacggtgat cctcgcccaa
      421 ctcgagggtc cgaaggcgct gccgatcccg gacgatctcc gccgcaagat gctcgactac
      481 ctcgagtagt atcgcctacg agcgcttggc cggccgcggg ccgacggtga agacctccag
      541 cacctcgccg cgaccgcgca cggcaagtcc gccgagcgag gtcaagcgct cgacaccgat
      601 gcggcttgcc gcctcggggc cgatgcagat gctcgtgccg agctccttgt tggcggcctc
      661 gagcctggcg gcgaggttca tcgcgttgcc gtgagcggtg tagtcgagcc gtcgcccgcc
      721 gcccacatcg ccgacgacga cgaggcccgt ctcgagtccg agccgtgtgc ggccaagcat
      781 gagtgccgcg ggctccgctt cgcgccggta agcctcagaa aaggcaagca gcgcctcggc
      841 gcaggcgagg cccttctgcg gatggttggt gagatcgagc ggcgcattga agatcgcgtg
      901 caggccgtcg ccgacgattt tctcgaccat gccgccgtgc tcgacgacaa tgcgcgaagc
      961 gccctcgatg tagccgtcga gaacgcgcac gagcgcgcgc ggatcgctgc gttccgtcat
     1021 cgacgtgaag ccttcgatgt ccgtgaacag cgccgtgatc tcgcgcatct cgccttcgag
     1081 gcggaggagc cccgggctct cgacgatgcg gcggacaact tcgggtgcca gatgctgctc
     1141 gaagctgcgt cgcacggcgg cctcgcggcg acgcgtgtgg gcggcggcga ttgtcgccgc
     1201 gacgccatat ccgagacccg cgactaccgg aggcgcaagc ggatcaatca gccatccgcc
     1261 gaaggcgatg ccggcgctcg cgagaacgag ccacacgagg ccgccgccga tggcaagggc
     1321 cgctgccttc atcgccgacc agcacaatcc ggcgcgcacc agaagtccgc cgaaaccgag
     1381 catcatggca gcctcgagcc agcccgcaat cgccggtcga tacgggaggt cgccggcgag
     1441 aagctggctg gccgcgtcgg cgtgaagctg taccgatgcg acgagattgc cgccggcggt
     1501 ggtgcgaagg cccgcaagct cgggcgcgga gctgccgagg aagacgattg cgcccttgat
     1561 gcgggcgcgt gccgcggcat cggccagcac ggcttgcgcg gagagggtgc gcccggcgtg
     1621 acgcgtttgg cttgttggac gcagcctgag cgaggcatcg cggctgaagg cgaagaggcg
     1681 cgggccgagc cgcgctgaaa acggactcga ctcgagcacg taggagcccg tgcccgacgc
     1741 cagccgtatt gcttcgagcg cgagcccagg ccgcagcaag ttgcccgccc tcaagatgag
     1801 gggcacggag cggatgcgac cgtccggatc ggccgccatg acgagtgcgc cgaagccttg
     1861 cgccgcctcg gcgatgatct ccgtcggtcc gatggcgccc tcggccgtcc agatgtcgcg
     1921 cacgtctggt gtgccacgaa cgaggatggg cgctggcggc agcagagttc cggccccatc
     1981 cggatcgagg acgaatccga cagccgtagg tgccttgcgg agagcggcgg caagccgcgt
     2041 gtcggcgtcg ggaagcttgt cgacgagggc ggcgacgccg gcatccttcg tgacgtcggc
     2101 gaggcgacgt gcgagagccg cgggcgagcg gtcgtccggt ccctcgatca gcatgtcgat
     2161 ggcgagcact gcgggagcgg cctcggcgat ccggtcgacg atgagggcga gctgctctcg
     2221 gctgatggag ttgccgcccc ccgcggtgag cgtcgcgcgg tcgagatcga cgacaatgac
     2281 cggtggtgtc ggctgaggcg gagcacgcgc ggctaggaag gcgtcgaatg tgcgctcacg
     2341 cagcgtctga acggccgaga tcggcagcag caccgccgcc gtcgccgcga gtagcatcaa
     2401 ccccatcagc gcagatggcc tgagtaccgt cgctcgcgcg atgtcaatga gcggtgccag
     2461 gtactgccga gtagcggcga gcgcggttcc gagaacttgc acgcggctca ctcctcatct
     2521 ttcgctattg cgcagcgcca caacaggatc gcatgtcggc acgtctcggc agccgtcgcg
     2581 gggtcgtcga tgcagactaa cggggcgatg ccgcggcggc atccatcgat tgtccagatc
     2641 gtggccaagc cacaggaccg gcccatgcag cctccttgcc aaaaaggcaa ttcagtcccg
     2701 gctcggagcg ccgagcggga acaaggagcg gaagggccgg gcctcatcga cggcgcgggc
     2761 gaaagagggg cgctgcagga gccgggcacg gtaggctcgc agcacgggaa aatcatgtcc
     2821 gatcggatgc gtccagtctg cgtagaagag tgatggtgcc gcggcgcaat cggccatcgt
     2881 gaaggcatcc ccggtcgccc acggccggcc ggcaagctcg gtctcgagcc aggcataggc
     2941 ccgcttcagc cggccggcgg cgacggctat gccgtcctca cgcttgatcg gcagccgccc
     3001 aagagcagct tcgacggcga tctgcatggc gtccatgacg tggaaatcga agtagcgatc
     3061 gaggaaccgt acggcgagcg cccggttcgg gtcggcaggg atgaggcgcg ccttgccagg
     3121 atggtggatg tcgaggtgct cgatgatgat gctcgattcg gcgatactcg cgtccccatc
     3181 gacgagcagc gggaactttc ccatcggcca gcggcgcgac cagtcgcgga cgtggtccgg
     3241 agtatcgggt ccgatgcagc ggaactcgaa ggccgtgtcg ctttcgtaga gggcgatcag
     3301 caccttctgc gtatacgacg agaagggatg accgtagagt gcaagtgcca ttggagaacc
     3361 tcgtcagccc ggtgccgcgc atccaagcga ttggaacgtg aggcgtgacg ttagcacgtt
     3421 tggcgtatcg cgcctgggac cgaggccacc gctcgttgga ccgcgcctgc aatcgtcgct
     3481 agtctcgcga cgaagaggcg acgaacagga gaggtcacca tgatctatga gctcaggatc
     3541 tacacagtaa aggctggaac tgtcggcgag gtcgccaaga actcgggcac cgtcgcgcgc
     3601 tccattcggg gtgacgacta cggcaagctc gagggctact ggatcaccga gatcgggccg
     3661 ctcgaccagg ttgtccatct gtggagctac aaggacctga acgagcgcca gcgcctgcgc
     3721 accgaactcg gcaagaatgt gcgctggacg aacgagtatc tgccgctgat ccgcccgcac
     3781 atgctgcgcc aggacatccg cctcctcaat catgtgatcg gcgtcaatcc gccggccaag
     3841 cagggcagca acgtctatga actccgctac tatcgcacgc gtcccggcgg cgtgaaggag
     3901 tgggtcggcc acttcagcga ggcgctgccg gtgcgcgaga agcactcgaa gatcgtcggg
     3961 ctctggactg gcgaggctgg ccagccaaat gaggtcgtgc acatctgggc gtacgccgat
     4021 ctcaacgcgc gtgccgcggc tcgcaatgca gcgatccagg atcccggctg gcaggccttc
     4081 ctgaagaagg cgacgccact gctcgacgag atgaactcag tgatcatgct gccggccgcg
     4141 cattcgccgc tcaagtaatg gggcgccgct gaagtaggcg gttgctcggg ctcaccaaac
     4201 tccgcgccgg agcccgttcc agaggtagcg ccagatcgtc ggcgggaacc actgccggga
     4261 cgggaggtcg acggtcgtcg tcgccacccg gcccgagcgc gcctcgccga tcgcgtgcat
     4321 gaaaaccggc aatgccacgc cgagctgggc gtactgatag gttgcaatcg tatcgcgcgg
     4381 gtcgaagcgg acgggagcct gcgcgatcac cttccccgtg tcgacgcctt gatcgacgag
     4441 atggatggtg atgccggcat gctcggcatc gccggaggcc agcgcccagt aggcggggtc
     4501 ctggccgcga tatttcggat tgatgccggc gtgatagttg atgaagggcg cctcgattgc
     4561 cgagagcgtc gcccgcgaga tgatgcgcgt gccgtagacg gcgaccaccg acgggcgcag
     4621 ccgtctcagc agatcacggc aggcctcgga attcaccgag gacaccagct cgatcctgag
     4681 gcccttgccg aagtcggtcc ggaatccgta cgtgcggtcg agctcggcga agcggcggcg
     4741 gttgaggcgg ccggcaacgc gggtcagcag gccgttggcg acctgaccga gggccgtcgg
     4801 ccaaccgagg aggcgggcac gccgctgaat gaccacgctc ttgcgctccg gctcttcgac
     4861 aatcactaca aggcggtcga agcggtcggc gaacgcattc gcgacgatcg ctcccatgcg
     4921 cccgtcggcg acgagcagga cgaggggagc agacggaccc gtagcgtgag cggcgacagg
     4981 cggcgtgtgt gccacgccgg gacaccgtgc gctacgctcg acattcattt gttcaatcac
     5041 ggaacgcaga cccggtagta gcccagtctc cggccgtggc gacgcaaatt ctgctccaca
     5101 ttgccgcctg agcgcccggc cgtgcgcact gccgagttgc tgccgcaggt cagccggtgg
     5161 tgagcacgag aataacgcca ttggcgtcgg aggggtgtac cttcacgacc cccggctctg
     5221 tcgtcgtctt ccggccggcg agggcagctg ctgcggcctc gtcgtcgccg acgcgcaaca
     5281 cgacggtgac ggcgccctct gccggcaggc cgtcaagcga aatgccggga tggcgaccgg
     5341 cgagcgtttc gcgatcgagg aacannnnnn nnnnnnnncc ccgcttgcga cgcggaaggc
     5401 cccgtccggc tccgcctcga tcggcatgtc gatcatgcgt gacatgtgat cagccgctgc
     5461 ccggggggcg gtcgaaagaa gctcgagccg ctcgatgcgt cgcgccgtat tcgcgtggcc
     5521 gagcagttcc ggcagccaga cgttttcgcg cgtgaaatgc tggcaggcga agatgcgcaa
     5581 gcctcccggg cgctggtcga cgggccattg cgtcgtctcg aagcgggcgt cggacttgac
     5641 accatcagct cgctcgacgg gtcggctgaa ggccacggga ccgatcggcg ggaaaccgtt
     5701 ggcggccagc tcctttgcaa ggccggccgc cgaacgggcc gtgaatgcgg cccgttcgat
     5761 gccttcgccg cggcgatcga ggaaggcgcg cgtcggcacg ttgcgctctg tctcggcgac
     5821 gacaccgatg agctcgatat agtcctcatc gagcatgatg gtgtggttgg ccgtacccat
     5881 atgcgcgctg tgcaggcccc tgtccgacac cttgaagccg aggcggcgcc agttctccgc
     5941 cgctgcggcg aggtcgcgga cgacgatgac gatgtggtcc agtccggcca cgttggcgag
     6001 gctcatggca ttccccttgg cgtcttggct cgtggtgcga tgatgcctca gggtttcaga
     6061 cgaggcgagg gtctgtcaaa ctccgcgcta gcgggtacgg cgctgcccga tgccggcgcg
     6121 ccggcaggcg gccttgactc tggagccggc gtggatatgg tgcgcaacac tttgacgacc
     6181 gccgaaagag gatcgcatgg caaagcccat cacccagaaa atcaagctgc tgagcacggc
     6241 gggcactggc tttttctacg tgaccaagaa gaacccgagg acgagcacag acaagttcgt
     6301 cttcaagaag tatgacccga ttgcccgaaa gcatgtcgag ttcaaagaga caaagatcaa
     6361 gtaagagccg tcgcgacctg ctcacgatga gcacgtgagg cgccctggca gccgcggcat
     6421 cagtgctgtg gcaggagtgg attgtcatct cgtcggcggc atgccgatcg gggaagactg
     6481 caggcctgtt tcgacagctc ggtgcggaga ggcgtcgcgg attgacgttg cggcacggct
     6541 gcatgtgccc gaagagtgac ggcgcggacg gattgaatgg actgcaatcg tcgaagatct
     6601 cgagcggctc aaggagccgc ggccgcggtc tgtggccaat ctcggcagcg gggtaaagat
     6661 ccgagcagcc acagaccacg gcctgtcgag cggtgaccaa agagggcgtt ggtcggtcac
     6721 tcgacaactg gtcgacgtta tgcggctttc aatgaggagg ccactccagc cactttcgtc
     6781 gaccttacac ccttggagcc aggagatgcg gcggacctga gatcctcggg tgttcctgaa
     6841 ttgatgatcc tgaattggcg ctcaggagtt gcgttgccct gtcgtactct ctccggtcgc
     6901 ccggagatcc gcatttgtat taggcagtct gattaaaccg cccacccttc ctcagcgcgc
     6961 atcagaataa cgttgctcat cctgaggcgc aacaactttc tagttgacag gctcgaatcg
     7021 tttctcagca tgtacctgag acgctacaac gacaagttga cgctactgtg acatgaattt
     7081 gataagttgt ggcgccggac tttaggcgga agaatatcat tccccgcgag ccgcgctcca
     7141 ccgggctgcc aaataggtta atatctggcg tcttcgcgtc aggccgccgc ggtgaccacg
     7201 cggttgcgcc cctcgcgctt ggcagcatag agcgcgaggt cggcgcgctt cagcagtgcc
     7261 atcggtgtgt cgtcgcgccg ctcgagcgtg gcgagaccga cgctcgccgt gacgcgcagc
     7321 tgcgtcgacg aattgatctg gaaggggtct gccgcgatac aggcgcgcag gcgctctccg
     7381 acctggcgcg caacggcgag atcggtatcc ggcatgatga ttacgaactc ctcgccgcca
     7441 aggcggcagg cgaggtcgat gccgcgcgtg ttgcggcgga atcgctgtgc gaactcgacg
     7501 agcacagtgt cgcccgcatc atgaccgtag gtgtcgttga ccgacttgaa gtagtcgata
     7561 tcggcgacca gcaccgaaag gtgccggttc ccggcaatcg catccgccac cagcgtcttg
     7621 aggtggcctt ccatatagcg gcgattgtgg agccccgtca ggctgtcggt gatggcgagt
     7681 tccacactct gcttgagccg cgtcgacagg aggtcctggt gccgcttgcg gcgcacctga
     7741 gatctgacgc gcgccagcat ctcgttcctg tcgatcggcc ggataaggta gtcgttcacc
     7801 cccatgtcga ggccgcgcag cagccgcgcg tcgtcgccgg gctcgaccag aatgatgatc
     7861 ggcagatgtc gcgtgcgctc gagcgagcgg acttgcgagc acagacgcaa gccgtcggat
     7921 tcggcgaggc tcaggctcac gaccaggaga tcgaaattct cctcggccgc tcgcagcagg
     7981 gccgcttgcg gatccttctc gagcgcaatc tcgtgcacct tgccgagggc ttcggtgagg
     8041 cgctgtccgg aacgggggtg atcctcgacg aggaggatgc ggcccttgtc actgccgccc
     8101 gcgagcagct ggtcgattga gggcaggccc atctgctcgc tggtcgaggc gcgcatgatg
     8161 agctcgtcgg agagtacctt gaggcgcgcg aggttcttca cacgcgtgat gagggcgatg
     8221 tcgtcgacgg gcttcgtcag gaaatcgtcg gcaccggatt cgagaccctg cagcttgtcc
     8281 gacggctgat cgagcgccgt caccatcacg atcggcagat gcgcggtgcg cgggttcgac
     8341 ttgatgatcc ggcaggcctc gaagccgttc attcccggca tcatcacatc gaggaggacg
     8401 acatcgacgc gctcgcgact gcagatctcg atggcgtcct tggcgttgaa ggcggtcagc
     8461 acctcgaagt attcggccga cagccgggcc tcgagcagct tgacgttggc gaggatgtca
     8521 tcgacgacga gtacgcgcgc tgtcatcagg aatgtcccat cgtctgcgtc cggccgctgg
     8581 cggcgggccg gccttcgcct atgaaccgct gcacggcctc tatgaagcgg ctgatcgaga
     8641 tcggcttcga gaggtagccc tcgcagccgc cgcgacggat gcgctcctcg tcgcccttca
     8701 tcgcgaaggc ggtgacggcg atcacgggga tcgtgcgcag cgcgtcgtcc tccttgatcc
     8761 agcgcgtcac ttcgagtccg gagacctcgg gcaactggat gtccatgatg atgaggtcgg
     8821 ggcggtggct cctcgccagc gcgagggcct cgatgccgtt gccggtgcgc aacgttgcaa
     8881 agccattggc atcgagcaga tcgtggaaca gcttcatgtt gagctcgttg tcctcgacga
     8941 tgaggacgag cttctttccg cccggaggac ggcgcgcttg aatgctgttg ctagtttcct
     9001 gataggtcat gacttggcgc gcccctcgcc cggtcgctgc cggagctgat tggcgcgagt
     9061 gtgcgcgaaa agcctgacca actgcttaag attgcgttgt caccccatca tgcgagcagc
     9121 agagccttgc ctcatgccct gcgggcgaaa cgcgataaac aaatggcttc caaaggccga
     9181 ggcctttggc ggggttcggg gcag
//
LOCUS       DAORNH010000183         9144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5607_length_9144_cov_2.593245, whole genome shotgun sequence.
ACCESSION   DAORNH010000183 DAORNH010000000
VERSION     DAORNH010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5607_length_9144_cov_2.593245"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2718
                     /gene="mfd"
                     /locus_tag="PK264_16775"
     CDS             <1..2718
                     /gene="mfd"
                     /locus_tag="PK264_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRD77562.1"
                     /translation="MEHWLPMFHPELETLFDYVGDAPISIDHLVEEAIGQRLEQIAEH
                     YKARLEAGEQQAFGAPPYKPVPTAQMFLDRKEWQAAVASRGLRRLSPFEAVEGKGANR
                     AFRGRVGRTFAAERTAETLNLFDALADHVRSLMGAGKRVIIAGWTLGARERLASLVAD
                     HGLPGAKKIESWPEALATAQGTLLLAVLGLERGFETPELAVIGEQDILGDRLVRPRKR
                     SKKAADVISEATSLSVGDLVVHADHGIGRFVGLTTITALGAPHDCLELAYHGGDKLYL
                     PVENIELLSRYGSDETGADLDRLGGVGWQTRKARLKKRLREIASELIKIAALRQLKEA
                     PAIAPPEGAFDEFVARFPFEETEDQAASIEAVIADLGAGRPMDRLVCGDVGFGKTEVA
                     LRAAFVAAMAGIQVAVVVPTTLLARQHFRTFSERFKGLPLRIAQASRMVPAKELAEVK
                     AGLKDGKVDIVVGTHALLAKSIEFMRLGLLIIDEEQHFGVGHKERLKQLREDVHVLTL
                     TATPIPRTLQLALSGVRELSLITTPPIDRLAVRTYISEVDPVVLREALRRERYRGGQT
                     FYVCPRIADLDDISEFLAEAVPELRVGRAHGQLSPTELEDVMTAFYEGKYDVLLSTAI
                     VESGLDVPNANTLIVHRADMFGLAQLYQLRGRVGRSKTRAYAYFTTPAGKRLTEGAEK
                     RLKVLHSLDTLGAGFSLASHDLDIRGAGNLLGEEQSGHIREVGFELYQSMLEEAIAAL
                     KDGGLEEAAETWSPQIALGTPVLIPEAYVTDLQVRLGLYRRLSSLETREAIDGFGAEL
                     VDRFGPLPGEVEQLLDVVEIKALCRRANIASVDAGPKGMSITFRKNQFPNPEGLVSIV
                     QAKRGLIKLMPDHKLVQKADWEAASARLAGVRAFVAELAGVAGGGRKAA"
     gene            complement(2836..3840)
                     /locus_tag="PK264_16780"
     CDS             complement(2836..3840)
                     /locus_tag="PK264_16780"
                     /inference="COORDINATES: protein motif:HMM:NF014247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HRD77563.1"
                     /translation="MTALTMSLMRTVTIRKLASTLAAAGVTLSAATPAFAAGDAVEVP
                     RQPWSFAGFFGRYDAAQLQRGYQVYQEVCSNCHGIKRVYFRNLAEAGGPGFPEDRLKS
                     LVAAKFKVDDAPNDQGKVLKRAALLSDAIPAPFKNDKEARSANGGSLPPDLSVITKAR
                     SVEYIGPWYLHPFAMLRDIGSGYQEHGPDYVYALLTGYGEAPLYKRDDKGHLVKLAKG
                     APAAGAERCASITPGEKGKPDECVKVANGMNYNVVFPGHQIAMPMPPFPTEKRAKYPA
                     GVTATVEQQARDVVAFLAWTADPSLNQRKQMGMLVMIYLLITAVLLYLAKKKLWSRVH
                     "
     gene            complement(3850..>4999)
                     /locus_tag="PK264_16785"
     CDS             complement(3850..>4999)
                     /locus_tag="PK264_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HRD77564.1"
                     /translation="LNYAYTFGGILSIMLVAQIITGIVLAMHYVPHADMAFNSVQLLR
                     RNVNYGWLIQSVHAVGASMFFIAVYIHMFRGLYYGSYKAPREILWILGVVIYLLMMAT
                     GFLGYTLPWGQMSYWGVTVITNLFSAIPFVGDSIVKWLWGGYSVSTATLNRFYSLHYL
                     LPFVIAGVVGLHIWALHVVGQNNPTGIEIKSKSDSVPMSPYATMKDLFGLSVFVMMFA
                     WFVFFAPDYLGHADNYTEANPLVTPPHIVPEWYYLPFYAILRAVPDKLGGVILMFSAI
                     LVLAFLPWLDTSRVRSAKYRPIFKLFFWLFVLSCLALGYLGSKPADGIYVMLARMFTI
                     YYFAFFLIIMPIVGVLETPSRLPRSITEAVLGPGGGTPAGAATAPEKR"
     assembly_gap    5000..5048
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5120..5698)
                     /gene="petA"
                     /locus_tag="PK264_16790"
     CDS             complement(5120..5698)
                     /gene="petA"
                     /locus_tag="PK264_16790"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HRD77565.1"
                     /translation="MASHSEEPSRRDFLTIAAGSWAAVGAAATLWPLVQQMNPDASTQ
                     ALASIEVDVAQIKEGQAIVVMWRGKPVFIRNRTKAEIEKAQAVAIGELSDKSARVEGR
                     PAGLPAVDQNRVKKGKENWLVLVGICTHLGCIPKGTEDKDRSFRGDYGGWFCVCHGSH
                     YDESGRIRKGPAPRNLDVPPYEFVADTKIKIG"
     gene            6019..6498
                     /locus_tag="PK264_16795"
     CDS             6019..6498
                     /locus_tag="PK264_16795"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRD77566.1"
                     /translation="MPSVRIAIFEPDIPQNTGTLLRLAACLGLAVDLIAPAAFDMSER
                     AFRRAGLDYLQHVTVERHASFEAFLAVSRRSGRRIVLLSTKAATSYLELVYSIDDVLL
                     VGRESAGVPEHVHAAADTRVRIPMRAGMRSLNVAVAAAMVLGEAMRQTGGLAQSRPG"
     gene            complement(6514..6873)
                     /locus_tag="PK264_16800"
     CDS             complement(6514..6873)
                     /locus_tag="PK264_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HRD77567.1"
                     /translation="MSSPTAPAPKITYDDFQKVDIRVGTIVEVEPYPEARKPSYKLKI
                     DFGPEVGIKKSSAQITKHYKPETLVGRQVAAVVNFPPRQIGKFMSEVLTLGFPDEANE
                     VVLVAPGHKVPNGGRLF"
     gene            complement(6996..>7627)
                     /locus_tag="PK264_16805"
     CDS             complement(6996..>7627)
                     /locus_tag="PK264_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77568.1"
                     /translation="AAERPWREVTLADIADAAASSLADVKREVASKSQVVAAFAGMVD
                     DEVLRRAPRRQADQPARDRLFEVIMTRFDVLQPYRSALKSISGAGLPDPELIRPFLAS
                     QAWMLESAGISASGLGGGVRALGLAGIYSQVVRIWLDDDDPGQARTMAALDRRLRRAG
                     ETIGRVEEMCSGVSRVLSSILPVFGGRRTTTETSAQPPDDPGRAADPAR"
     assembly_gap    7628..7646
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7745..8227)
                     /locus_tag="PK264_16810"
     CDS             complement(7745..8227)
                     /locus_tag="PK264_16810"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRD77569.1"
                     /translation="MTSRLMILNGPNLNLLGVREPHIYGRTTLASIETSCVALAGTLG
                     ASLEFLQSNHEGVLVEHIHRARTTADALIMNPAGYSFTSIAIFDALKAFEGPYIEIHI
                     SNIHARDELHRHSILSRAARGVICGLGPYGYLVAIQAALQMLGKTPEALPAALRKGPE
                     "
     gene            complement(8261..8764)
                     /locus_tag="PK264_16815"
     CDS             complement(8261..8764)
                     /locus_tag="PK264_16815"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD77570.1"
                     /translation="MSDVIVRELIDREAIKELKARYCRFVDQQQWERLRGLFAPDTRF
                     DGFNSAPAGSTVDQFIAGISARFVGVVSIHHVHSPEIAFTGSNSARGIWPMMDYLQFP
                     PTAVMKEAPGMRGFVGYGHYEEEYVRTAEGWRFSLLRLTRIRIDPLGPDLPPVQRGML
                     MADPSWL"
     gene            complement(8820..>9144)
                     /locus_tag="PK264_16820"
     CDS             complement(8820..>9144)
                     /locus_tag="PK264_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77571.1"
                     /translation="SKVLDRREETPLKQERQVMKRKFTLAMAAVALLSVAILSTGCQM
                     LKARDELNKGVTAYKNAKYADAVERAWHLGRERARLEWHDQVQLKGAEADTLAKLLRF
                     RRPAC"
BASE COUNT         1556 a         2873 c         3111 g         1536 t
ORIGIN      
        1 atggagcact ggctgccgat gttccaccct gaactggaga cgctcttcga ctatgtgggg
       61 gacgcgccga tctcgatcga tcacctcgtc gaggaggcga tcggccagcg cctcgagcag
      121 atcgcggagc actacaaggc gcgcctcgag gccggcgagc agcaggcctt cggcgcgccg
      181 ccctacaagc ccgtaccgac ggcccagatg tttctcgacc ggaaggagtg gcaggcggcc
      241 gtcgcctcgc gcgggctgcg ccgcctctcg cccttcgagg cggtcgaggg caagggcgcg
      301 aaccgggcgt tccgcgggcg agtcgggcgc acctttgcgg ccgagcggac ggccgagacc
      361 ctcaatctct tcgatgccct cgccgaccac gtccgatcac tcatgggcgc ggggaagcgc
      421 gtcatcatcg cgggctggac gctcggcgcg cgcgagcgcc tcgcctcgct cgttgccgat
      481 cacggattgc cgggtgcgaa gaagatcgag agctggccgg aggcgctggc gaccgcgcag
      541 ggcacgctgc tcctcgccgt gctcggcctc gagcgcggct tcgagacacc ggaactcgcc
      601 gtgatcggcg agcaggacat cctcggcgac cggcttgtgc gtccgagaaa gcgttcgaag
      661 aaggccgccg acgtcatcag cgaggcgacg agcctctcag tcggcgatct cgtcgtgcac
      721 gccgaccacg gcatcggccg cttcgtcggc ctcaccacga tcaccgcact cggcgcgccg
      781 cacgactgtc tcgaactcgc ctatcacggc ggcgacaagc tctacctgcc cgtcgagaat
      841 atcgagcttc tctcgcgcta cggctcggac gagaccggcg cggatctcga ccggctaggc
      901 ggtgtcggct ggcagacgcg caaggcccgc ctcaagaagc ggctccgcga gatcgcctcc
      961 gagctcatca agatcgcggc actccgccag ctcaaggaag cgccggcgat cgcgccgcct
     1021 gagggcgcct tcgacgagtt cgtggcgcgc ttccccttcg aggagaccga ggaccaggca
     1081 gcgagcatcg aggccgtgat cgcggacctc ggcgcgggcc ggccaatgga ccggctggtg
     1141 tgcggcgacg tcggcttcgg caagacggag gttgcattgc gagcagcctt tgtcgcggcc
     1201 atggcgggga tccaggtggc cgtcgtcgtg ccgaccacgc tgctcgcccg ccagcacttc
     1261 cgcaccttct cggagcgctt caaggggctg ccgctcagga tcgcccaggc ctcgcgcatg
     1321 gtgccggcaa aggagctcgc cgaggtgaag gcgggcctca aggacggcaa ggtcgatatc
     1381 gtcgtcggca cgcacgcgct gctcgcgaag agcatcgagt tcatgcggct cgggctcctc
     1441 atcatcgacg aggagcagca tttcggtgtc ggccacaagg agcgcctgaa gcagctccgc
     1501 gaggatgtgc atgtgctcac cctcacggcg acgccgatcc cgcgcacact ccagctggca
     1561 ctgtcgggcg tgcgcgaact ctcgctcatc acgacgccgc cgatcgaccg tctcgcggtg
     1621 cgcacctaca tctccgaggt cgatcccgtc gtgctgcgcg aggcgctccg gcgcgagcgc
     1681 tatcgcggcg gccagacctt ctacgtatgc ccgcgtatcg ccgacctcga tgacatctcc
     1741 gagttcctcg ccgaggcggt gccagagctg cgcgtcggcc gcgcgcacgg ccagctctcg
     1801 ccgaccgagc tcgaggacgt catgacagcc ttctacgaag ggaagtacga cgtgctgctc
     1861 tcgacggcga ttgtggaaag tggcctagac gtgccgaacg ccaacacgct gatcgtgcat
     1921 cgcgccgaca tgttcggcct tgcccaactc taccagctgc gcggccgtgt cggccgctcg
     1981 aagacgcgtg cctatgccta cttcaccacg ccggccggca agcgtctgac agagggcgcc
     2041 gagaagcgcc tcaaggtgct ccactcgctc gacacgctcg gcgccggctt ctcgctcgcc
     2101 tcccatgatc tcgacatccg cggcgcaggc aacctgctcg gcgaggagca gtcgggccac
     2161 atccgcgagg tcgggtttga actctaccag tcgatgctcg aggaggcgat tgcagcattg
     2221 aaggacggcg ggctcgagga ggctgccgag acctggtcgc cgcagatcgc gctcggtacg
     2281 ccggtgctca ttccggaggc ctacgtcacc gacctgcagg tgcggctcgg gctctacaga
     2341 cgcctatcga gcctcgagac gcgcgaggca atcgatggct tcggcgccga actcgtcgac
     2401 cgcttcgggc cgctgcccgg cgaggtcgag caactcctcg acgtcgtcga gatcaaggcg
     2461 ctctgccggc gtgccaacat cgctagcgtt gatgcggggc cgaagggcat gtcgatcacg
     2521 ttccggaaaa atcagttccc gaaccccgag gggctcgtct cgatcgtgca ggcgaagcgc
     2581 ggcctcatca agctcatgcc cgaccacaag ctcgtgcaga aggcggactg ggaagcggcc
     2641 tcggcgcgtc ttgccggcgt ccgtgcgttt gtcgccgagc tggcgggcgt cgcaggcggc
     2701 gggcggaagg cggcgtgaat cgtctggcgt gggcagtcgg ggggccgggg cgctgccggc
     2761 aggctcatga tctacggaaa agcgaaggcc ccgctcggag gcggggcctt gactgtgcga
     2821 gcctgagccc gggcgtcagt ggacgcgcga ccagagcttc ttcttggcga ggtagagcag
     2881 cacggcggta atcagcaggt agatcatcac cagcatgccc atctgcttgc gctgattgag
     2941 gctcggatcg gccgtccagg cgaggaacgc gacgacgtcg cgggcctgct gctcgacggt
     3001 ggcggtcacg cccgcggggt acttggcgcg cttctcggtc gggaaggggg gcatcggcat
     3061 cgcgatctgg tgacccggaa acacgacgtt gtagttcatg ccgtttgcga ccttcacgca
     3121 ctcgtcgggc ttgcccttct cgccgggcgt gatggacgcg cagcgttcag ctccggccgc
     3181 cggtgcgccc ttggcgagct tcaccagatg acccttgtcg tcgcgcttgt agagcggcgc
     3241 ctcgccatag ccagtgagca gtgcgtacac gtagtccgga ccgtgctcct ggtagccgct
     3301 gccgatgtcg cgcagcatgg cgaatggatg caggtaccag gggccgatgt actcgaccga
     3361 gcgggccttg gtaatcaccg agaggtccgg cggcagcgac ccgccgttgg cactgcgcgc
     3421 ttccttgtcg ttcttgaacg gcgcggggat ggcgtccgag aggagagctg cccgcttcaa
     3481 caccttgccc tgatcgttcg gggcgtcgtc gaccttgaac ttggcggcga cgagcgactt
     3541 caagcgatcc tccgggaaac ccggtccgcc ggcttccgcc agattgcgga agtagacgcg
     3601 cttgatgccg tggcagttgg agcagacctc ctggtagacc tgatagccgc gctgcagctg
     3661 ggcggcatcg tagcggccga agaagcccgc gaagctccag ggctggcgcg ggacctctac
     3721 ggcgtcaccg gccgcgaagg caggcgtggc agccgacagg gtgacgccgg ctgcagcaag
     3781 cgtggacgcg agcttgcgga tcgtgactgt gcgcatgagg gacatggtga gtgctgtcat
     3841 gattggacct cagcgcttct cgggagcggt ggcagcgccg gcaggcgtgc cgccgcccgg
     3901 accgagcacg gcttccgtga tcgagcgggg cagccgagac ggggtctcga gcacgccgac
     3961 gatcggcatg atgatgagga agaaggcgaa gtagtagatc gtgaacatgc gggcgagcat
     4021 cacgtagatg ccatcggcag gcttcgatcc gagatagccg agcgcgaggc acgacagcac
     4081 gaacaaccag aagaagagct tgaagatcgg acggtacttg gccgagcgca cgcgcgacgt
     4141 gtcgagccaa ggcaggaagg ccagcacgag gatcgccgag aacatcagga tgacaccacc
     4201 gagcttgtcc ggcaccgcgc gcaggatcgc gtagaacggc aggtagtacc actccggcac
     4261 gatgtggggc ggggtcacca gcggattggc ctcggtgtag ttgtccgcat ggccgagata
     4321 gtcgggcgcg aagaacacga accatgcaaa catcatcacg aagaccgaga ggccgaagag
     4381 gtccttcatg gtcgcgtagg gcgacatcgg aaccgaatcc gacttcgact tgatctcgat
     4441 gccggtcgga ttgttctggc cgacgacgtg cagggcccag atgtgcaggc cgacgacgcc
     4501 agcgatcacg aagggcagga gatagtggag cgagtagaag cggttgaggg tcgctgtcga
     4561 gaccgagtag ccgccccaca accacttcac gatcgagtca ccgacgaagg ggatcgccga
     4621 gaagaggttg gtgatgacgg tgacgcccca gtagctcatc tggccccaag gcagtgtgta
     4681 gccgaggaag ccggtcgcca tcatgaggag gtagatgacg acgccgagga tccagaggat
     4741 ctctcggggc gccttgtaag agccgtagta gaggccgcgg aacatgtgga tgtagacggc
     4801 gatgaagaac atcgacgcgc cgacggcgtg cacgctctgg atgagccagc catagttcac
     4861 gttgcggcgc agcagctgca ccgagttgaa ggccatgtcg gcgtgcggca cgtagtgcat
     4921 ggcgagcacg atgccggtga tgatctgcgc caccagcatg atcgagagga tgccgccgaa
     4981 ggtgtaggcg tagttcaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnncg tcgagccact gctcgacgcg cgtgcccggc ttgtaggtgg agggatggtc
     5101 tgccatgggc gtctgtctct cagccgatct tgatcttggt gtcggcaacg aactcgtagg
     5161 gcggcacgtc gaggttgcgg ggcgccggac cttttcggat gcggccggat tcgtcgtagt
     5221 gtgagccgtg gcagacgcag aaccagccgc cgtagtcgcc gcggaacgag cggtccttgt
     5281 cctcggtccc cttcgggatg cagccgagat gcgtgcagat gccgacgaga acgagccagt
     5341 tctccttgcc cttcttcacg cggttctgat cgaccgctgg caggcctgcg ggccggccct
     5401 cgacgcgcgc gctcttgtcg ctgagttctc cgatcgccac ggcctgcgcc ttttcgattt
     5461 cggccttcgt gcgattgcgg atgaacacgg gcttgccacg ccacatcacg acaatcgcct
     5521 gcccttcctt gatctgggcc acatcgacct cgatcgaggc aagcgcctgg gtcgaggcgt
     5581 cggggttcat ctgctggacg agaggccaga gcgtcgccgc ggcgccgacc gccgcccagc
     5641 tgccggccgc gattgtcaag aaatcacggc gtgacggctc ttccgaatgg gacgccaagc
     5701 ttgcctcctt ctcgcttctc aaggctcgcg gccatggcca atcgagccat ctgatgcggt
     5761 catttcgatg cggccgacga gcaccctcga gcgcgccctg ccgaccggtg ccatcttcgc
     5821 gccaccacag tgtcgcagga gatgacgctc gctcgccatg gctgcgcccc gtacatggcc
     5881 ggcctgcgct tccgggagtg cgcggcctcg atatgccgtg caccagtgac gtgcggtgtt
     5941 ttggcatcct ttgccatgac cgacaaggct tggcggtcgc ccgcaagatg ggcaatttgg
     6001 cgcatcgcct tcaggtggat gccaagcgtg cggatcgcga tcttcgagcc cgacatcccg
     6061 cagaacacgg ggacgctgct gcggctggcg gcctgcctcg gccttgcggt cgatctcatc
     6121 gcgccggcgg ccttcgacat gagcgagcgc gcatttcgcc gtgccggcct cgactatctc
     6181 cagcatgtga ccgtcgagcg gcatgcttcg ttcgaggcat ttctcgcagt gagccgcaga
     6241 tcggggcggc gtatcgtgct gctctcgacc aaggccgcga cgagctactt ggagctcgtc
     6301 tactcgatcg acgatgtcct gctggtcggg cgcgaaagcg ccggggtgcc ggagcacgtg
     6361 cacgcggcag ccgacacgcg ggtgcgcatc ccgatgcgcg ccggcatgcg ctcgctcaat
     6421 gtcgcggttg ccgcggccat ggtgctgggc gaggccatgc ggcagacagg cgggctcgct
     6481 caatcgcgcc ctgggtgatc gttcgagtac cgtctagaac aacctgccac cgttcgggac
     6541 cttgtggccg ggggcgacga gcacgacctc gttggcctcg tccggaaacc cgagtgtcag
     6601 cacctcggac atgaacttgc cgatctggcg cggcgggaag ttaacaacgg cggcaacctg
     6661 acggccgacg agcgtctcgg gcttatagtg cttcgtaatc tgggccgaac tcttcttgat
     6721 gccgacctcc ggaccgaagt cgattttcaa cttgtaagat ggcttgcgcg cctccgggta
     6781 cggctcgacc tcgacgatgg tgccgacgcg gatgtcgacc ttctggaagt cgtcataggt
     6841 gattttcggc gcgggcgcag tgggcgagga catgagttga tccggggctc gaaagacgat
     6901 gcgaccgcag ggcggccaaa ggggccgcac cagggttgct cggagcctct gatatcagaa
     6961 gggggccggg acgcaaagcg cgtcgccgtg cagaattacc gtgccggatc ggcagcgcgg
     7021 cccggatcat cgggcggttg cgctgaggtc tcggtcgtgg tccgcctgcc gccgaagaca
     7081 ggcagaatgc tcgagagcac gcggctgacg ccgctgcaca tctcctccac gcgcccgatg
     7141 gtctcgcccg cgcgacggag gcggcggtcg agggctgcca tcgtccgcgc ctgcccgggg
     7201 tcgtcgtcgt caagccagat gcgcacgacc tgcgagtaga tgccggcaag cccgagggcg
     7261 cgcacgccgc cgccgagccc gctcgcactg atgcccgcgc tctcgagcat ccaggcctgc
     7321 gaggcgagga acgggcggat cagctcgggg tctgggaggc cagcccccga gatcgatttc
     7381 agcgcgctcc gatagggctg gagcacgtcg aagcgagtca tgatcacctc gaagaggcga
     7441 tcgcgagccg gctggtcggc ctgtcgacgt ggagcacgtc tcagtacctc gtcgtcgacc
     7501 atgccggcga aggccgcgac gacttgagac ttggaggcga cctcgcgctt cacgtcggcg
     7561 agcgagctgg cggcagcgtc ggcgatatcg gcgagcgtca cctcacgcca gggccgctcg
     7621 gcggcgannn nnnnnnnnnn nnnnnngatg cggcccttcg gcgttgcgtg gtcgagcatg
     7681 aggctgatcc cgggctacct gtttcgatag atgagcatgt ccaagccgtt gcgcaaggta
     7741 cctgttactc cggccctttg cgcagcgccg cgggcaaggc ctccggcgtc ttgccgagca
     7801 tctgcagcgc cgcctgtatc gccaccagat atccgtacgg accaagcccg cagatcacac
     7861 ctctggccgc acgtgagagt atcgaatgac ggtgcagttc gtcgcgggcg tggatgttag
     7921 agatgtggat ctcgatgtag gggccttcga aggccttcaa ggcatcgaag atggcgatag
     7981 aggtgaacga gtagccggcg gggttcatga tcagcgcatc ggccgtcgtg cgggcccggt
     8041 gaatgtgctc gacgagcacg ccttcgtggt tcgactgcag gaattcgagc gatgcgccga
     8101 gagtgccggc gagagccacg cagctcgtct cgatgcttgc aagtgtcgtg cggccataga
     8161 tgtggggctc gcgcaccccg agcagattga ggttcgggcc gttcaggatc atcaggcggg
     8221 aggtcattgg tcgctccaca gatgcgggtt gggtcggcga tcaaagccag ctcgggtcgg
     8281 ccatcagcat gccgcgctgc acgggcggaa ggtcggggcc gagcggatcg atgcggattc
     8341 gcgtcaggcg aagcagcgag aagcgccagc cctcggcagt gcggacatac tcctcctcgt
     8401 agtgcccgta gccgacgaag ccacgcatgc cgggcgcttc cttcatgacg gcggtcggcg
     8461 ggaactgaag gtagtccatc atcggccaga tgccgcgggc tgagttcgag ccggtgaacg
     8521 cgatctcggg cgagtgaacg tgatggatcg agacgacgcc gacgaagcgt gccgagattc
     8581 cggcgatgaa ctggtcgact gtcgatcccg cgggggcgga gttgaagccg tcgaagcgag
     8641 tgtccggcgc gaagagcccc cggagccgtt cccactgctg ctgatcgacg aaacgacagt
     8701 agcgcgcctt cagctccttg atggcctcgc gatcgatgag ctcgcggacg atgacgtctg
     8761 acatgtgtgg ctccggtttg acgcatcaag cgcgtctgcg taggcgacgt gtcagctatt
     8821 cagcacgcgg gccgacggaa acgcaaaagt ttcgctaagg tgtccgcttc cgctcctttc
     8881 agctggacct gatcatgcca ctccagacgc gcacgttctc gcccgagatg ccatgcacgc
     8941 tcgacggcat ccgcgtactt ggcattcttg tacgcggtaa cgcccttgtt gagttcgtca
     9001 cgggctttca acatctggca gcccgtggac agaatcgcga cgctgagcag agccaccgcg
     9061 gccattgcga gtgtgaactt acgcttcatc acttgtctct cctgtttcag tggggtttcc
     9121 tcgcgcctgt caagcacctt ggac
//
LOCUS       DAORNH010000184         9141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5611_length_9141_cov_2.695135, whole genome shotgun sequence.
ACCESSION   DAORNH010000184 DAORNH010000000
VERSION     DAORNH010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5611_length_9141_cov_2.695135"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            451..1341
                     /gene="panB"
                     /locus_tag="PK264_16825"
     CDS             451..1341
                     /gene="panB"
                     /locus_tag="PK264_16825"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRD77572.1"
                     /translation="MSKEPAKAEAKKREKRTISEIRDSKKTGEKFVYMSVPDYTAAQW
                     AEMAGVDVAVVGDSLAMIAHGHKSTIPASMDMMVMHAQAIRRGAPNTFVLGCMPYQSY
                     NTVDRALVNATRFMQDALTDAVKPQGGKSQAHILKALVDAGIPTASHIGLTPHTIAMF
                     GGFKIQGRTADAAMKILEDALAIEDAGCFMLEFEAVPAKIAKLISEQLTIPTIGIGAG
                     VGTDGQILLCHDLLGVFTDFKPKFTKRFAKLTEVAVAGIKEYIKEVKEGSFPDDDHSY
                     TVKEEEYEKFARMVQKRKQI"
     gene            complement(1532..>2178)
                     /locus_tag="PK264_16830"
     CDS             complement(1532..>2178)
                     /locus_tag="PK264_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HRD77573.1"
                     /translation="SRSGGYARFVAGQSFHLAGENPYTTPGSILIDGVAQPVFSANSG
                     LGTDRSDYVIGAYLAPVDIFRFAGQVRLDEHDLSLQRADLYAVLRLGPVSAGVNYSLA
                     AADPSVGLFGTQQDLYGTISLELTKNWSIAAYARYDLDDRFLLQDSIRVSYKDECFVL
                     TASYTETFISNPTLDLKPDRAIMLTFELKHIGQFKYKSDALDHVFGENQTSIPK"
     assembly_gap    2179..2188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2189..3946)
                     /gene="lptD"
                     /locus_tag="PK264_16835"
     CDS             complement(<2189..3946)
                     /gene="lptD"
                     /locus_tag="PK264_16835"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HRD77574.1"
                     /translation="MTEGWAERTAAGAPMRAARLLVCLLAGLVALVAASVSSHVNAQQ
                     PDPPPLIAPPSKSAFAKPKDGLFGKPKALDNSKPLLLNADELIYDTKNNRVIARGNVE
                     AYYNDYILTADQIVYDQTANTLTAEGNALLKEPNGNVVRGQRLIASVDFTEAFVQSLS
                     IVARDDTRIVAERATRRDGNVSEYERAKFTPCKSDGTSPPLWCLSAARIVHDQRAATL
                     TYQDAQFELFGVPILYMPYFQHADPTVKRRTGFLLPEIRHSTQLGYQIEVPYFFALAP
                     NYDFLFHPLYTSKQGILWQGDWRHRVQWGQVSGQYTVKLAGIDQDDQDERAAGPLASR
                     WRGSLETKALFSLSSWWSFGWDVTLESDDSFRRFYKLDSILQTDRVNSVFLVGLSERN
                     YFGMHLYHFGGLLLDDTPHAESRVHPVIDYDYVFNRSVLGGEMSFSANALSLSRSDGT
                     DMTRGNFEARWRRQIIDGIGQVFTPYLAVRGDLYRYTNYTDPNTLLPVPEDSIARAMG
                     TAAMTYAYPFVAHTHVGSHVVEPTAQIIYRPARVTQRVLPDEDARSLIFDDTLLFDYD
                     KFSGWDRIETGTRANVGLNY"
     gene            complement(3959..5056)
                     /gene="lptG"
                     /locus_tag="PK264_16840"
     CDS             complement(3959..5056)
                     /gene="lptG"
                     /locus_tag="PK264_16840"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HRD77575.1"
                     /translation="MIRPRTLRRYVARRFLLTIIGTFGLCAVLIFMIDIVELLRQAGK
                     FGRVPSLRIAWMALLRLPAYTEILMPFAVLVGTIGALLLLSRKSELTVMRAAGMSVWQ
                     FLRPGLLVAAAIGILSTVAYNPMAAAARVESERLFAEAFGREMTTVLKSDGHGAWLRQ
                     DGLDGATVIHAGAVGSKGMQLTKVAVFQFDAGGRFVERIDALRANLKERHWELEEATV
                     FRTGLKPETYSAFTISTYLTPERVADAIGSSFSSSFWELPGLIEVAEKASLPSHRLKT
                     QYQQLLSRPALLVVMVLLGATVSLRSFRGGGIQTMVITGMIGGFGFFLLSEVSRQVGV
                     AGLASPVLAIWIPIAVAGCVSLLVLFRQEDG"
     gene            complement(5053..6249)
                     /gene="lptF"
                     /locus_tag="PK264_16845"
     CDS             complement(5053..6249)
                     /gene="lptF"
                     /locus_tag="PK264_16845"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HRD77576.1"
                     /translation="MPSLLSRYIFRQAAGTVLLILLSLSGVVWIALALRQLNLVTTQG
                     QDTLMFLKMTLLALPNLMALIAPIALLVATIHVLNRLNGDSELIIATAAGGTIWNIAR
                     PLLVLALLVAVAVAAVNHFAQPWSLQLVREYIIQIRTDLINQVIQPGRFTPPEPGVMF
                     HIRDRTLDGELQGILVHDARNDKEVTTYLADRGRIIKQPGGAYLLMQKGHILKREKLD
                     EPVRIIEFENAPIDLARFEQKGEVIELKPRERYFSELVRPDPKDPIFLRQPGLLRAEL
                     HERFASLLYPFAFVLIAVAFVGQAQTTRQNRMEAIIVAFVIAAACRLGGLAATNVVAL
                     RASALPLLYGIPIAAIFAGAIAAHLRMRARRQSRLRAMIDNAVDALLARLPRFGRKPA
                     QKGAAS"
     gene            6714..8204
                     /locus_tag="PK264_16850"
     CDS             6714..8204
                     /locus_tag="PK264_16850"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HRD77577.1"
                     /translation="MARRLEISFVSLDSPTETTVVLLAADGPEFGPVARRLDQSAGGA
                     LVKAAGAADFSGKAKASIEVLAPAGLGARRVVMLGIGEGGKGTDLERQGLGGVLLSSV
                     HGRRMIAASVIVDLKGEAATLWAADLAMGALLRSYSFDRYKSKREEGSSIERLVVHCD
                     QPERAEEEFARRQAVAKGVFLARDLVNEPANVLGPVEFAERANELADTGLEVEVLDVP
                     ALEALKMAALLGVAQGSVRPARVVVLQWAGAKSKRARPIGLIGKGVVFDTGGISIKPA
                     GGMEDMKGDMAGAAAVTGAMLALAARKAHVNAVGIIGLVENMPSGCAQRPGDIVRSMS
                     GQTIEVINTDAEGRLVLADLLWYAQERFKPRFMIDLATLTGAIMVALGKDFAGLFSND
                     DKLAAEIARAGEATGERVWRMPLDRSYERLIESKTADMKNIGGRHGGACAAAQFLGRF
                     VKDTPWAHLDIAGTAMDSSRTDLNQGWASGWGVRLLDRLIADAHEK"
     gene            complement(8208..8675)
                     /locus_tag="PK264_16855"
     CDS             complement(8208..8675)
                     /locus_tag="PK264_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77578.1"
                     /translation="MLLQSPKDPKSGPPEGAREPTTIRPGHWLSSVLIIDDKTAEAAS
                     VAARLHLLLGYDIRIEHAKGLGRALDFLQKDCAPNLIMLDDVLPPTDRAPSSIAFLRR
                     AGYHGPIIVVGGLLTTAIIGAARAAGAIDVIHKDDLDTARLLDAIGKLTPPRS"
     gene            complement(8894..9139)
                     /locus_tag="PK264_16860"
     CDS             complement(8894..9139)
                     /locus_tag="PK264_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77579.1"
                     /translation="MAAAVAGCAAQSAPYDPGARPLPSGQSCQSLRGELNKLDARGVP
                     ARVQAAAEGRKLPPAQQAEVDRYNSLLGAYLGARCHT"
BASE COUNT         1662 a         2774 c         3166 g         1529 t
ORIGIN      
        1 cagcggcagg ttgggccggg gttagctgag gacggcccgt ccgacatcgc ccccgatcac
       61 ggaaacaccc agcacgcgta tccgtcgtcc atgaccgttg ggtcgcctga gcatagccgc
      121 gcccgccaag tccgtggata agtctgttca cgtctcttcg caactggggc gccggcccag
      181 acccgcctgg ggctaaggcc gccccagacc cggcctcttc tgaatcttac gtcaccacga
      241 gcttcaggtc ggatgctgat cgcccgtcga ggttccagta gtgctcctga gcgctgtgga
      301 actgtaggcc tcgaaaaagg tggatttggg aggctctgcc tcccgccgga gcgcggggct
      361 ggtctcgagt tgcgctctgc gctcccctcg cttgccggct cggccgcgcc cccttacagt
      421 caaatatatc agatgctgcg ggagggtccc atgtccaagg aaccggcgaa ggccgaggca
      481 aagaagcgcg agaagcggac gatcagcgag atccgcgaca gcaagaagac gggcgagaag
      541 ttcgtctaca tgtctgtccc ggactatacc gccgcgcagt gggccgagat ggccggcgtc
      601 gacgttgccg ttgtcggcga tagtctcgcc atgatcgccc acggccacaa gtcgaccatc
      661 cccgcatcga tggacatgat ggtgatgcac gcgcaggcca tccgccgcgg tgcccccaac
      721 accttcgtgc tcggctgcat gccctatcag agctacaaca ctgttgaccg cgcgctcgtg
      781 aacgccacgc gcttcatgca ggacgcgttg accgacgccg tgaagccgca gggcggcaag
      841 tctcaggcgc acatcctgaa ggcgctcgtc gacgccggca ttccgaccgc atcccatatc
      901 ggcctcacgc cgcacacgat tgccatgttc ggcggcttca agatccaggg ccggacggcg
      961 gatgcggcca tgaagatcct cgaagatgcg ctggcgatcg aggacgcggg ctgcttcatg
     1021 ctcgaattcg aggcggtgcc ggcaaaaatc gcgaagctca tctcggagca gctgaccatc
     1081 ccgacaatcg gcatcggcgc cggcgtcggc accgacgggc agatcctcct ctgccatgac
     1141 ctcctcggcg tgttcacgga cttcaagccg aagttcacga agcgctttgc caagctcacc
     1201 gaggtcgcgg tcgcagggat caaggagtac atcaaggagg tgaaagaggg ctcattcccg
     1261 gacgatgatc actcctacac ggtgaaggag gaggaatacg agaagttcgc ccggatggtg
     1321 cagaagcgaa agcagatctg agcggcgaaa cgttctcgaa ttcgcggcag ccgtgcggcc
     1381 ggtgtcgcgc cttgcgacgt tgtaggaatt gggtcggttg gcgccgactg ggccgcgggc
     1441 ccagacccgc ctggggcttt gaccgccccc gacccggccc catctaagga tgcccgctgt
     1501 ctcgccaaga tttgatgccg cccaggcgag cctacttcgg gatgctcgtc tggttttcgc
     1561 cgaagacatg gtcgagcgcg tcggacttgt atttgaactg gccgatgtgc ttcagctcga
     1621 aggtgagcat gatggcgcgg tccggcttca ggtcgagtgt ggggttcgag atgaacgtct
     1681 cggtgtagct cgccgtcagc acgaagcact catccttgta cgagacgcgg atcgaatcct
     1741 gcagcaggaa gcggtcgtcg aggtcataac gggcgtaagc cgcgatgctc cagtttttgg
     1801 tgagctcgag cgagatcgtg ccgtagaggt cctgctgcgt gccgaacagg ccgacggagg
     1861 ggtcggcggc ggcgagcgag taattgacgc cggccgacac ggggccgaga cgcaggaccg
     1921 cgtagagatc ggcgcgctgg agagagaggt cgtgctcgtc gagtctgact tgcccggcga
     1981 agcggaagat gtcgaccggg gcgagatagg cgccgatcac atagtcggag cgatccgtac
     2041 cgaggccact gttcgcggag aacaccggct gcgcgacacc gtcgatcagg atgctgcccg
     2101 gcgtcgtgta agggttctcg ccggcgagat ggaagctctg gccggcaaca aaccgcgcgt
     2161 agccgccgct ccgcgactnn nnnnnnnngt agttcaagcc gacattggcg cgtgtgccgg
     2221 tctcgatccg atcccagccg gagaacttgt cgtagtcgaa gagcagcgtg tcgtcgaaga
     2281 tgaggctccg cgcatcctcg tctggcagca cgcgctgcgt gacacgggcc ggacggtaga
     2341 tgatctgggc ggtcggctcg acgacgtgcg atccgacgtg cgtgtgggcg acgaagggat
     2401 aggcgtaggt catcgccgcg gtgcccatgg cccgcgcgat cgaatcctcc ggaaccggca
     2461 agagcgtgtt cgggtcggtg tagttggtgt agcggtagag atcgccgcgg acagcgaggt
     2521 agggcgtgaa cacctggccg atgccgtcga tgatctgccg gcgccaacgg gcctcgaagt
     2581 tgccccgggt catgtcggtg ccgtcgctgc gcgagaggct cagcgcgttc gccgagaagc
     2641 tcatctcacc gccgagcacg gagcgattga acacgtaatc gtagtcgatg accgggtgaa
     2701 cgcgcgattc agcgtgcggc gtatcgtcga gcagcaatcc gccgaagtgg tagaggtgca
     2761 tgccgaagta gttgcgctca gacaagccga cgaggaacac cgagttcacg cggtcggtct
     2821 ggaggatgct gtccagcttg tagaagcggc ggaaactgtc gtcgctctcg agcgtcacgt
     2881 cccagccgaa cgaccaccac gacgagagtg agaacagcgc cttggtctcg aggctgccgc
     2941 gccagcggct ggcgagcggg cccgcggcgc gctcgtcctg gtcgtcctgg tcgatgccgg
     3001 cgagtttcac ggtgtactgg ccgctgacct gaccccactg cacgcgatgg cgccagtcgc
     3061 cctgccagag gatgccctgc ttggaggtgt agagggggtg gaacaggaag tcgtagttcg
     3121 gcgcgagcgc aaagaagtag ggcacctcga tctggtagcc gagttgcgtc gaatggcgga
     3181 tttccgggag caggaagccc gtccggcgct tcacggtcgg gtccgcatgc tggaagtagg
     3241 gcatgtacag gatcggcacg ccgaagagct cgaactgcgc gtcctgatag gtgagggtcg
     3301 cggcacgctg gtcgtgcacg atgcgtgcag cgctgaggca ccagagcggc ggcgatgtgc
     3361 cgtcgctctt gcagggcgtg aacttggcgc gctcgtattc ggagacgttg ccgtcgcggc
     3421 gcgtcgcgcg ttcggcgacg atgcgcgtgt cgtcgcgggc gacgatgctc agcgattgaa
     3481 cgaaggcttc cgtgaaatcg accgaggcga tcaggcgctg gccgcgaacg acgttgccgt
     3541 tgggctcctt gaggagcgcg ttgccctcgg cggtgagcgt gttggcggtc tggtcataga
     3601 cgatctggtc ggcggtcagg atgtagtcgt tgtagtaggc ctcgacgtta ccgcgggcga
     3661 tcacgcggtt gttcttcgta tcgtagatga gttcgtccgc gttgagcagc agcggcttcg
     3721 agttgtcgag ggccttgggc ttgccgaaga ggccgtcctt gggcttggcg aaggcgctct
     3781 tcgagggcgg ggcgatcagc ggcggcgggt cgggctgctg ggcgttgaca tggctcgaga
     3841 ccgaggctgc gacgagcgcc acgagacctg cgagaaggca cacgagcagc cgcgcggcgc
     3901 gcattggcgc accggctgcc gtccgttcgg cccatccctc agtcacgcgc aaccccactc
     3961 agccgtcctc ctgtcggaag agaaccaata gcgacacgca ccccgctacc gcgatcggga
     4021 tccatatagc caggacggga gacgccaaac ctgcgacacc cacctggcgt gagacttccg
     4081 acaacaggaa gaacccaaat cccccgatca ttcctgtgat gaccatagtc tggatgccgc
     4141 cccctcggaa tgacctcaaa gacacagttg cccccaaaag gaccatcacc acaagcaggg
     4201 ccgggcggga cagcaactgc tggtactgcg tcttcagacg atgcgaggga agagaggcct
     4261 tctcggccac ctcgatgagg cccggaagct cccagaatga gctgctgaac gatgatccaa
     4321 tcgcatccgc aacccgttcg ggtgtgaggt atgtgctgat ggtaaatgcg ctgtaggttt
     4381 ctggcttcag gcctgttcgg aaaacggtcg cctcctcgag ctcccaatgc cgctccttga
     4441 ggttggcgcg cagggcgtcg atgcgctcga cgaaccgccc gcctgcatcg aactggaata
     4501 cagcgacctt ggtgagctgc atgcccttgg acccgaccgc gccggcgtgg atcaccgtcg
     4561 ccccgtcgag gccgtcctgg cggagccatg caccgtgtcc atcggacttg aggacggtcg
     4621 tcatctcccg gccgaaggcc tccgcaaaga ggcgctcgct ctcgacgcgg gcggcagcgg
     4681 ccatcggatt gtacgcaacc gtcgagagta ttccgattgc cgccgcaacc aggaggccgg
     4741 ggcggaggaa ctgccacacc gacatcccgg cggcgcgcat cacggtgagc tccgacttgc
     4801 gtgacagcag gagaagggcg ccgatggtgc cgaccagaac agcgaagggc atcaggatct
     4861 ctgtgtaggc gggaagcctc aggagcgcca tccaggcgat ccgcagagat ggcacacgtc
     4921 cgaacttgcc ggcctgccgc aagagttcga cgatgtcgat catgaagatc agcaccgcgc
     4981 agaggccgaa cgtgccgatg atcgtcagca ggaagcggcg agcgacatag cggcggagcg
     5041 tacgcggccg gatcatgagg ccgcgccttt ttgcgcgggc ttgcgcccga agcggggcag
     5101 gcgtgcgagg agggcatcga cggcgttatc gatcatcgcc cggagacggg actggcgtcg
     5161 ggcgcgcatc ctgaggtggg cggcgatggc cccggcgaaa atcgcggcaa tgggaatgcc
     5221 gtagaggagg gggagggcgc tcgccctgag agccacgacg tttgtcgctg cgaggccgcc
     5281 gaggcggcag gcggccgcga tcacgaaggc gacgatgatc gcctccatgc ggttctggcg
     5341 tgtcgtctgg gcctggccga cgaaagctac ggcgatcagc acgaaggcga agggataaag
     5401 gaggctcgcg aagcgctcgt gcaactcggc gcgcaggagg cccggctggc ggaggaagat
     5461 cgggtccttc ggatcgggac gcacgagctc ggagaaatag cgctcgcgcg gcttcagctc
     5521 gatgacttcg cccttttgct cgaagcgggc aagatcgatc ggcgcattct cgaactcgat
     5581 gatgcgcacc ggctcgtcga gcttctcgcg cttcaggatg tggcccttct gcatgaggag
     5641 ataggcgccg cccggctgct tgatgatgcg gccacgatcg gcgaggtagg tcgtcacctc
     5701 cttgtcgttg cgggcgtcgt ggacgaggat gccctgaagc tcgccatcaa gggtgcgatc
     5761 gcggatgtga aacatcacgc cgggctcggg cggcgtgaag cgccccggtt ggatcacctg
     5821 attgatgagg tcggtgcgga tctggatgat gtactcgcgg acgagctgca ggctccaagg
     5881 ctgcgcaaaa tggttgacgg cggccaccgc gaccgcaacg aggagtgcga gcacgagcag
     5941 cgggcgggcg atgttccaga tggtaccgcc ggcagcggtg gcaatgatga gctcactgtc
     6001 gccgttcagc cgattgagga cgtggatcgt cgcgaccagc agcgcgatcg gcgcgatcag
     6061 cgccatcagg ttcggcagcg cgagcagcgt catcttcagg aacatcagcg tgtcctggcc
     6121 ctgcgtggtg accaggttca gctggcggag cgcgagcgct atccacacga cgcccgagag
     6181 cgagagcagg atcagcagca ccgtgcccgc ggcctgtcga aagatgtatc gggagagaag
     6241 cgaaggcatc agctgcccct cgcaaggcag ggacgggcgc gccggtcgca actgccgagc
     6301 cgccacgctg agagcgcggg cgggtgcccg atggtcccaa gatccgaaga gaggagcatg
     6361 ccctcgtccg tctccatgct ggcgccgtgt cccccttagg tcttgcctcg ttccggcctc
     6421 ccccgtcagc ctgcagccaa gcggcgcgat ctgccatgcg gcagcacgac cgcgacgcag
     6481 gttgacgacc gggcacatgg agttcgcgcc aaaatcgggc cgaaatccgg cgcgctgcgc
     6541 tataggccct agcctcgatt gtgacgccat ggcaaggcgt cgatcgggtc gccaacaaga
     6601 cgtgagggct gaaagggtcg tgcacggcca gaagccgcat gataaaggcg gcgtcgatcc
     6661 gggtccccct ggcttcgctc cacgtcgatt tcgttcacag atggactctc cccatggctc
     6721 gccgcctcga gatatcgttc gtctcactcg acagcccgac tgagacgact gtcgtgctgc
     6781 tggccgccga tggccccgag ttcggcccgg tcgcccgccg gctcgatcag tcagccggcg
     6841 gggcgctcgt aaaggcggca ggtgccgccg atttcagcgg caaggcaaag gcgtcgattg
     6901 aggtgctggc gccggctgga cttggcgcgc ggcgggtcgt catgctcggc atcggcgaag
     6961 gggggaaggg taccgacctc gagcggcagg gtctcggcgg cgtcttgctg tcgagcgttc
     7021 acggccggcg gatgatcgcc gccagcgtaa tcgtagacct caagggggag gcggcgaccc
     7081 tctgggctgc agacctcgcc atgggtgcac tgctccgctc gtacagcttc gaccgctaca
     7141 agtcgaagcg agaggagggg agttcgatcg agcgactcgt cgtgcattgc gatcagccgg
     7201 agcgggctga agaggagttt gcgcggcggc aggccgttgc caagggcgtg tttctggcgc
     7261 gggacctcgt gaacgaacct gcgaatgtgc tcggtccggt cgagtttgcg gagcgcgcaa
     7321 atgagctcgc cgacaccggc ctcgaggtcg aggtcttgga tgtgccggcg ctggaggcgc
     7381 tcaaaatggc cgcactcctc ggcgtcgcgc aggggagcgt ccggccggcg cgggtcgtcg
     7441 tcctgcagtg ggccggagca aaatcgaagc gcgctcggcc gatcggcctc atcggcaagg
     7501 gcgtggtgtt cgacaccggc ggcatttcga tcaagcccgc tggcggcatg gaggacatga
     7561 aaggcgacat ggcgggtgcc gcggcggtca cgggtgccat gctggcgctc gccgcacgca
     7621 aggcccatgt gaacgccgtc ggcatcatcg gcctcgtcga gaacatgccg tcaggctgtg
     7681 cgcagagacc cggcgacatc gtccgctcga tgtcggggca gacgatcgag gtgatcaaca
     7741 ccgacgccga gggccggctc gtgctggctg atctcctctg gtatgcgcag gaacgcttca
     7801 agccacggtt catgatcgat ctcgcaacac tgaccggggc gatcatggta gcgctcggca
     7861 aggatttcgc gggtctcttc tccaacgacg acaagcttgc cgcggagatt gccagggccg
     7921 gcgaggcgac aggtgagcgg gtgtggcgca tgcccctcga caggagctac gagcggctca
     7981 tcgaaagcaa gaccgccgac atgaagaaca tcggcgggcg ccatggcggg gcctgcgcgg
     8041 cggcccagtt cctcggtcgc ttcgtcaagg acacgccctg ggcgcatctc gatattgccg
     8101 gaacggccat ggactcgtct cgcaccgacc tcaaccaagg ctgggcgtca ggctggggcg
     8161 tcaggcttct cgaccggctc attgcggatg cacacgagaa gtgagcgtta cgatcgcgga
     8221 ggcgtcagct tgccgatcgc gtcgaggagg cgtgcggtat cgagatcgtc cttgtgaatg
     8281 acatcgatcg cgcctgcagc gcgggcagcc ccgatgatgg ccgtcgtcag caatcctccg
     8341 acgacgatga tcgggccgtg atagccggca cggcgcagga atgcgatgct gctgggcgcc
     8401 cggtcggtcg gcggcagaac gtcgtcgagc atgatcaggt ttggcgcgca gtccttctgc
     8461 aggaaatcga gggcgcgccc gaggcccttg gcgtgctcta tacggatgtc atagccgagc
     8521 aggaggtgaa ggcgcgccgc cacgctggcg gcctctgctg tcttgtcgtc gatgatcaga
     8581 acgctgctca gccagtgccc gggcctaatc gtggtcggct cgcgcgcgcc ctctggcggt
     8641 ccggatttgg gatctttcgg cgactgcaga agcatattgg atcaggtccc tcgcgaactg
     8701 gcgtgccaga cttgaaaaac gcacgaagag aatcggacgc tgaacatcgt ctaatgccat
     8761 cgagggacga tgtcagacga agcactatcg ccgctttcgg gacctatccg cccaacgatt
     8821 gccacaatca aaaagaggcc gcccatgcgg gcggcctcag gaaatacgtg cgatatcaga
     8881 gtatctgtcg gcgtcaggtg tggcagcgcg cgccgagata ggcgccgagc agcgaattgt
     8941 agcggtcgac ctcggcctgc tgtgccggag gaagcttacg cccttccgcg gcagcctgga
     9001 ctcgggcggg cacgccgcgc gcatccagct tgttgagctc gcctctgagc gactggcaac
     9061 tctgacccga tggcagcgga cgggcgccag ggtcgtaggg cgcgctctgc gccgcacagc
     9121 cagctaccgc tgccgcgatc a
//
LOCUS       DAORNH010000185         9131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5620_length_9131_cov_2.479176, whole genome shotgun sequence.
ACCESSION   DAORNH010000185 DAORNH010000000
VERSION     DAORNH010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_9131_cov_2.479176"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK264_16865"
     CDS             <1..445
                     /locus_tag="PK264_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77580.1"
                     /translation="GKDAFYRGEIAREIVRSISIDGQSARDVAVKLGMSEGKLALEVH
                     SNQLRGDTYNGGFLDIGMIENPRLTLTPVGGGPPVTVVVERSIVGGKGTFTPLRQLHV
                     VPPGTYDIDFEAAVYPPVKLGVWPVDFTTRKPIKVRLATGHTLGR"
     gene            481..1179
                     /locus_tag="PK264_16870"
     CDS             481..1179
                     /locus_tag="PK264_16870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77581.1"
                     /translation="MSVDPKAFWEDKILTWEKGRYQPKDSSGSLLERIADASSRSLRY
                     RIKTGVELLTPHIKGKSVLEVGCGSGLIAQALIDAGASRYVGIDIAENAIAIANKRKA
                     ELKWSDRIAFKVGTIREMAPVAEDVVLSLGVLDWLTDEELEVLFQRQGKAQFMHAIAE
                     KRNSLSQLAHRAYVQVSYGYRTGAYRPRYFRAATIASLAGKHQPGPFYAFRNKQLSFG
                     ALISSLPVGERIAL"
     gene            complement(1189..1635)
                     /locus_tag="PK264_16875"
     CDS             complement(1189..1635)
                     /locus_tag="PK264_16875"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRD77582.1"
                     /translation="MSKSSVREGFAYVSVAGLAAGSDWLVFTLLSWLAPSSDVVLAQA
                     PARLTGGLVAFMMHRNWSFRDQQGQGMGTEAKRFLALYIFSFCLSIATIFLLVDVFAI
                     NRFASKAVADTLCFGVNFLVMKHYVFASVENVGEAASRLRTAARSR"
     gene            complement(1637..>2672)
                     /locus_tag="PK264_16880"
     CDS             complement(1637..>2672)
                     /locus_tag="PK264_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRD77583.1"
                     /translation="IQRLQELGADIAIITSGNGKWYFEGRPDIARMHEIEAFYYASKN
                     GRISIPRTLMSVGDFRRIARRNSDVVEHVLDDFKPDVALLDSVYTTGPLKKRGIPIVA
                     LNNADVVHVSYRRFTEKPRSIRMQFYAIEEMDYLFHRLRADLVLSPTFDDTLPEVGRN
                     IRRIGPIVRRGYSSDRHSGDVKRVLVMLSGSRFGSPVALKDRQFPFHVDVVGREAPKD
                     VVLPPSIVFHGRVKDNTEIVKSADLMLVNGGFSAVSEAFALRKPMVVLPIPNHAEQWV
                     NGRTIEGLGVGVMADENAIEAAMLKAITSISDFRRAYERLPALRDGAGEAARHIIETG
                     QKKSQQRKSQ"
     assembly_gap    2673..2754
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2755..3231)
                     /locus_tag="PK264_16885"
     CDS             complement(<2755..3231)
                     /locus_tag="PK264_16885"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77584.1"
                     /translation="MDLLGDVRGRDILELGCGAGFYTRLVLSRGAGHVWAVDLSERMI
                     AELPSEGVTGIHADATTANPGRSFDTLLSAGMLEFVPDPKAVLANAARFAAPNGRFAV
                     LMPTASMLGRIYQRFHRRHGLSINLFDSSQLSRLGGETGWTLANWVQAGPYSAAALL"
     gene            3577..>3861
                     /locus_tag="PK264_16890"
     CDS             3577..>3861
                     /locus_tag="PK264_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77585.1"
                     /translation="MQSTVAMQGIWRRIAEPGRALASAKPWSIDYLAVSTGFLLAFLL
                     IDWVTFTPAFAPRGVTPWNPAIGFALAVVLRYGLGLLPILVVAPLISDVVV"
     assembly_gap    3862..3871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3872..>4633
                     /locus_tag="PK264_16895"
     CDS             <3872..>4633
                     /locus_tag="PK264_16895"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HRD77586.1"
                     /translation="LPFPFQVVLLEAVISGVGYAGATWVLLKSRWRIAPRIETVRDLL
                     TLIITTSLATMLVAVATIAVLSSAGLMRPERAIPAFWRFWIGDIIGILVVLPFLLKVA
                     ADGNGPRVTSEPLAQLLAILGTIAIMFASPSGLRLPISYILFLPIVWIAVRGGLARVA
                     SGLVIMQIALMIAIHITADDPANIASYQALMVALSVSGLTIGVLTDERVRGERDLRRQ
                     QDAIARAARIGTLGEMATAVAHELNQPLTAAANYAR"
     assembly_gap    4634..4662
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4663..5245
                     /locus_tag="PK264_16900"
     CDS             <4663..5245
                     /locus_tag="PK264_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77587.1"
                     /translation="PALELLRETGLKTLEQVERSAAIIRRLRDYISVGRLEVGAHQAG
                     AIISECIELMRPEFDRSGTALEVYVPAPLPPLSVDRLQIETVLTNLLRNAVEAIEAEQ
                     PEAPHISISAIHILSGFVEIAVADNGIGFPSGFRLSRAGPIASTKAEGLGIGLSLCQS
                     IIESHGGRIEIDRRSGGATVRMTLPVAGGPAHA"
     gene            5238..5852
                     /locus_tag="PK264_16905"
     CDS             5238..5852
                     /locus_tag="PK264_16905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77588.1"
                     /translation="MRKPIVLAVVDDDPSVLDSTVILLRAQGLTVHAARSAEALLAAI
                     TAGTLKPDCIVCDVLLPGASGIDLIEALAAHGTRAPVVLITGHGDIRMAVSAIKMGAS
                     DFIEKPFAIEDLVASIDAAVARSRAAEDAARERSEIEGRVALLSARQREVMDLVVEGY
                     SSKQIGLKLGISPRTVETYRLQIMDKMGAGSVAALVRMTASIRR"
     gene            complement(5877..6770)
                     /gene="panC"
                     /locus_tag="PK264_16910"
     CDS             complement(5877..6770)
                     /gene="panC"
                     /locus_tag="PK264_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRD77589.1"
                     /translation="MASKGSSGAGASAAGTSATVQVARTVSELRGIVAGWRRAGETLG
                     LVPTMGALHEGHSALVRAAKSRCSRAVVSIFVNPTQFAPNEDFDKYPRTFDDDLAKVA
                     AAGGDLVWAPVVKEMYRDGAATRVVPKGAALGLETDFRPHFFEGVATVCCKLFTQVGP
                     DIALFGEKDYQQLAVVTQMVRDLDLPLQIVGHPTIREPDGLAMSSRNRYLSSDERARA
                     VTIHKVINDVAGAARSGDPAAAISAGEARLTAAGFGKIDYVAVRDAVTFGAYDRASGR
                     PGRVLAAAWLGKTRLIDNVAV"
     gene            7021..>9131
                     /gene="putA"
                     /locus_tag="PK264_16915"
     CDS             7021..>9131
                     /gene="putA"
                     /locus_tag="PK264_16915"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HRD77590.1"
                     /translation="MPTRDEISTYHLHDETRLVGALIERAVYTEDERRRIGEHARRLV
                     EAARADRRRHGGLDAFLAEYGLTSEEGVILLCLAEALLRIPDTQTADALIAEKMAGGQ
                     WERHLGASDSLFVNASTFGLMLTGKVVRLKDARGMNPLNLVKRMVARSGEPVIRQALR
                     QAMKILGDQFVLGRTIGEAIDRARSYERRGYRFSYDMLGEAARTARDAERYFSRYVTA
                     IEAVGKAAGPLASLHGDALMARPGLSVKLSAVHPRFEPGKEERLQRELVPRLRDLARR
                     AREAGLPLTVDAEEQDRLDLTLDAFAEVFLDPVLDGWPGLGVVVQAYGRRAIPVLRWL
                     KRLSDVSGKRIPVRLVKGAYWDSEIKWAQERGWTDYPVFTRKLHTDVSYLAAMRFLLS
                     DPVAFFPQFATHNAHSISSGLIAGGNRTFEFQRLHGMGEALYEAVVGDGTAGRVCRIY
                     APVGGHEDLLAYLVRRLLENGANTSFVNRLADEDTTAAEMVVDPVEAAEREWQSGQLG
                     AERTLPKPTELFEPRLNSVGLPLSEPRVRADAEKAIARELEVQIAAGPIVDGKVTAGG
                     DEARLALCPHDRRQRVGTVREATPEDIERSIAASLKAAHAWDRRGGHERAKILEAAAD
                     LYERNRMRLMAVIVREAGKTLENAQADIREAVDFLRYYAAEARRLFAGPVPLPGPTGE
                     RNTLELRARGPIAAIAPWNFP"
BASE COUNT         1519 a         3044 c         2933 g         1514 t
ORIGIN      
        1 gggtaaggat gccttctatc gcggcgagat cgcgcgcgaa atcgtccgct cgatctccat
       61 cgacgggcaa tcggcgcgcg acgtggcggt gaagctcggc atgagcgagg gcaagctggc
      121 gctcgaagtg cattccaacc agctgagagg cgacacgtac aacggcggct tcctcgacat
      181 cggcatgatc gagaatcccc ggctcacctt gacgccggtt ggcggcgggc cgcccgtcac
      241 ggtcgtcgtc gaacggagca tcgtcggcgg aaagggtacg ttcacgccgc tgcgccagct
      301 ccacgttgtg ccgccgggta cctacgatat cgacttcgag gcggcggtct acccgccggt
      361 gaagctcgga gtatggcccg tcgacttcac caccaggaag cccatcaagg tgcgtctcgc
      421 gacggggcat acgctcggac gctgaacggc gggtaacacc atgcggaagg atttagagtc
      481 atgagtgtcg atccaaaggc cttctgggag gacaagatcc tgacctggga gaagggccgc
      541 tatcagccga aggacagcag cggcagcctg ctcgagcgga ttgcggacgc ctcgagccgc
      601 tcgctgcgct atcgcatcaa gacgggcgtc gagctgctca ccccgcacat caagggcaag
      661 tccgtcctcg aggtcggttg cggctcgggg ctaatcgcgc aagctcttat cgatgccggc
      721 gcgagccgct atgtcggcat cgacatcgcc gagaacgcga tcgccatcgc caacaagcgc
      781 aaggccgagc tcaagtggag cgaccgcatt gcgttcaagg tcggcacgat ccgcgagatg
      841 gcgcccgtcg cggaggacgt ggtcctctcg ctcggtgtgc tcgactggct caccgacgag
      901 gagctggagg tcctcttcca gcggcagggc aaggcgcagt tcatgcacgc catcgccgag
      961 aagcggaact cgctctcgca gctcgctcat cgcgcctacg tgcaggtgtc gtacggctac
     1021 cgcaccggcg cctatcggcc gcgctacttc cgtgcagcga ccattgcgtc gctcgcgggt
     1081 aagcatcagc cggggccgtt ctatgccttc cgcaacaagc agctgagctt cggcgcactc
     1141 atctcgagcc tgccagtcgg cgagcgcatc gcgctgtagg atcgccagct agcggctccg
     1201 agccgcggtg cgcaggcggc ttgccgcctc gccgacgttc tcgacgctcg cgaacacata
     1261 gtgcttcatg accaggaagt tgacgccgaa gcacaacgtg tcggcgacgg ccttgctggc
     1321 gaagcgattg atggcgaaca cgtcgacgag caggaaaatc gtcgcgatcg agaggcagaa
     1381 gctgaagatg tagagcgcta ggaagcgctt cgcctcggtg cccatgccct ggccctgctg
     1441 atcgcggaac gaccagttgc gatgcatcat gaaggcgacg agcccgccgg tgagccgtgc
     1501 cggcgcctgg gcgagcacga cgtcgctgct aggggcaagc cacgacagca gcgtgaatac
     1561 gagccaatct gagcccgcag cgagccccgc cacggacaca taagcgaacc cttccctcac
     1621 actcgacttc gacacgctat tggctctttc tctgttggct tttcttctgg ccagtctcga
     1681 tgatgtgacg cgcggcctct ccggcaccgt cgcgaagcgc cggcagccgc tcataggccc
     1741 gccggaaatc gctgatcgat gtgatggcct tcagcatggc ggcctcgatc gcgttctcgt
     1801 cggccatgac gccaacgccg aggccctcga tggtgcgacc gttaacccac tgctcggcat
     1861 ggttcgggat cggcagaacg accatcggct tcctgagcgc gaaggcctcg ctgacggcgc
     1921 tgaagccgcc attgaccagc atcaggtcgg ccgatttgac gatctcggtg ttgtccttga
     1981 cgcgaccatg gaagacgatg ctcggcggca gcacgacgtc cttcggcgcc tcgcgaccga
     2041 caacatcgac atggaacggg aactggcgat ccttgagcgc cacaggtgag ccgaagcgcg
     2101 agccgctcag catcaccagc acgcgcttca cgtccccgga atgccggtcg gacgagtagc
     2161 cgcgccggac gatcggccca atgcgccgga tgttccggcc gacctccggg agcgtgtcgt
     2221 cgaaggtcgg gctaagcacg agatcggcgc gcagccggtg gaagaggtaa tccatctcct
     2281 cgatcgcgta gaactgcatg cggatcgagc gcggtttttc cgtgaagcgt cggtacgaga
     2341 cgtgcacgac gtcggcattg ttgagcgcga cgatgggaat gccgcgcttc ttgagtggac
     2401 cagtcgtgta gacagaatcg agcagtgcga cgtccggctt gaagtcgtcg agcacgtgtt
     2461 cgacgacgtc tgaattgcgc ctggcaatgc gacggaaatc accgaccgac atcagcgtgc
     2521 gcgggatcga gatgcgaccg ttcttcgagg cgtagtagaa ggcctcgatc tcgtgcatgc
     2581 gggcgatgtc cggccggccc tcgaagtacc acttgccatt gccggaggtg atgatggcga
     2641 tatccgcacc gagctcctgc aggcgctgga tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaatag
     2761 tgccgcggcg ctgtagggcc ccgcctgaac ccaattcgca agtgtccagc cggtttcgcc
     2821 gccgagcctg ctcaactgcg aactatcgaa caggttgatc gacaggccgt ggcggcggtg
     2881 gaaacgctgg taaatgcgtc cgagcatcga ggccgtcggc atcagcaccg caaaccggcc
     2941 attcggtgca gcaaaccggg ccgcgttggc gagcaccgcc ttcggatcgg gcacgaactc
     3001 gagcatgccg gcagacagca atgtatcgaa ggagcggccc gggttggccg tcgttgcatc
     3061 ggcgtggatg ccggtgaccc cctcactcgg caactctgcg atcatgcgct cggagaggtc
     3121 gaccgcccag acatgccccg cacctcggct gaggaccagg cgggtataga agccggcgcc
     3181 gcacccgagc tccagaatgt cccggccgcg gacatcgccg agaaggtcca tgagcacggc
     3241 ggcctcgcgc cggcgcaacg ctccccagat gcggccctgc gaggccctct ggtagccaga
     3301 ggccacgccc gaaaaatacg cttcgacctt acgagatcct gacatgaccc ccgtgccgcg
     3361 acccccagtg ccctaaccca agtgcccgaa accaagtgcc gtgcttgcct cagcaggccc
     3421 agtggcatcc gccatgtccc cgccggaagc cgtcgatcca gcctgaggat gctcgatcgt
     3481 cccgttcaga tcgaacgaga gatgagcttg tctatccacc ggttctgggc gaagttccgc
     3541 ctccgagata tccgtagaat tacgatggag cgccgtttgc aatcgactgt cgcgatgcag
     3601 ggcatctggc ggcgcatcgc ggaaccgggc cgggcgctgg catcggcaaa gccctggtcg
     3661 atcgactacc tcgcagtgtc gacggggttc ctgcttgcgt tcctgctcat cgactgggtc
     3721 acctttacac ctgccttcgc ccctcgcggc gtcaccccct ggaacccggc gatcggcttc
     3781 gctctcgccg ttgtgctccg gtacgggctc gggctcctgc ccatactagt tgttgcgcca
     3841 ctgatttccg atgtcgtggt gnnnnnnnnn ncctgccgtt tccgtttcag gtcgtgctgc
     3901 tcgaggccgt catctcgggc gtcggctacg ccggcgcgac ctgggttctg ctcaaatcgc
     3961 gctggcggat tgccccacgg atcgagaccg tccgcgatct tctgacgctg atcataacga
     4021 catcgctcgc caccatgctg gtcgcagtcg cgacgatcgc cgtgctgagc tcggccggcc
     4081 tgatgcgccc tgagcgcgcg attccggcat tctggcgctt ctggatcggc gacatcattg
     4141 gcattctcgt cgtgctgccg ttcctgctca aggtcgcagc tgacggcaat gggccgcgcg
     4201 tcacctccga gccgctcgct cagctcctgg caatcctcgg aacgatcgca atcatgttcg
     4261 cctcgccgag cggcctccgg ctgccgattt cctacatcct gttcctgccg atcgtctgga
     4321 ttgccgtgcg gggcggactg gcccgcgtcg ccagcggtct cgtgatcatg cagattgccc
     4381 tcatgatcgc catccacatc accgcagatg acccggccaa cattgcctcc taccaggcgc
     4441 tgatggtggc cctttcggtc tctggcctca ccatcggcgt gcttaccgat gagcgcgtac
     4501 gcggcgagcg ggacctgcgc cgccagcagg acgcgatcgc acgtgcggcg cggatcggca
     4561 cgctcggcga aatggcgacg gcggtcgcgc acgaactcaa tcagccgctg acggccgccg
     4621 ccaactacgc ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccggcgc tcgagctgtt
     4681 gcgggagacc ggcctcaaga cactcgagca ggtagagcgc agcgcggcca tcatccgccg
     4741 cctcagggac tatatcagcg tcgggcgcct cgaggtcggc gctcaccaag caggagcgat
     4801 catcagcgag tgcatcgagc tcatgcggcc ggagttcgac cggtccggca ctgccctcga
     4861 ggtttatgtg ccggccccgc tgccgccgct ctccgtcgat aggctgcaga tcgagaccgt
     4921 gctgaccaat ctcctgcgca acgcagtcga ggcaatcgag gcggagcaac cggaggcgcc
     4981 acacatctcg atctcggcga tccacatcct gagcggcttc gtcgagattg cagttgccga
     5041 caatggtatc ggctttcctt caggctttcg cctctcgcgg gccggtccga tcgccagcac
     5101 aaaggccgag ggcctcggaa tcggactttc gctctgtcag tccatcatcg aaagccatgg
     5161 aggtcggatc gagatcgatc gccgatcggg tggtgcgact gtgcgcatga cgcttccggt
     5221 tgccggcgga cctgcccatg cgtaagccga tagttctcgc ggtcgtcgat gatgacccct
     5281 cggtgttgga ttcgaccgtg atcctcctcc gcgcgcaggg tttaacggtc cacgcggcgc
     5341 ggtccgccga ggccctgctg gcggcaatca ctgcgggaac gctgaaaccc gactgcatcg
     5401 tttgcgacgt cctgctgccg ggcgcctccg ggatcgacct catcgaggct ctcgccgcgc
     5461 acggcacccg agcgccggtc gtgctcatta ccggccacgg cgatatccgc atggccgtgt
     5521 cggccatcaa gatgggggcg agcgacttca tcgagaagcc gttcgcgatc gaggatctgg
     5581 tcgcctcgat cgatgccgcg gtcgctcgct cgcgggccgc cgaggatgcc gcgcgcgagc
     5641 gctcggagat cgaagggcgc gtcgctctgc tctcggcacg gcaacgcgag gtcatggacc
     5701 tcgtcgtcga gggctattcg agcaagcaga tcgggctcaa gctcggcatc agcccgcgca
     5761 ccgtcgagac ctaccggctg cagatcatgg acaagatggg cgccggcagc gttgcagcgc
     5821 tcgtgcgcat gacggcgtcc atccgtcgct gagtgggtcg agagtgcgtc gctcgctcat
     5881 acggcgacat tgtcgataag ccgcgtcttg ccgagccagg cggcggcaag cacgcggccg
     5941 ggccggcccg aggccctgtc gtaggccccg aacgtcaccg catcacggac cgctacgtag
     6001 tcgatcttgc cgaagccggc agcggtgagg cgcgcctcgc cggccgagat cgcggcggcc
     6061 ggatcgccgg agcgggcggc gccagcgaca tcgttgatca ccttgtgaat ggtcacggcg
     6121 cgcgcccgct cgtcggacga gaggtagcgg ttgcggctcg acatggcgag accgtccggc
     6181 tcgcggatgg tcggatggcc gacgatctgg agcggcaggt cgaggtcgcg caccatctgc
     6241 gtgacgacgg cgagctgctg gtagtccttc tcgccgaaga gggcgatgtc cggaccgacc
     6301 tgcgtgaaga gcttgcagca gaccgtcgcg acgccttcga agaagtgcgg gcggaaatcg
     6361 gtctcgaggc cgagcgctgc gcccttcggc acgacgcgag tcgcggcgcc gtcgcggtac
     6421 atctctttga cgaccggcgc ccagacgaga tcgccgcccg cagcagctac cttggcgaga
     6481 tcgtcatcga aggtgcgcgg atacttgtcg aaatcctcgt tcggcgcgaa ctgcgtcgga
     6541 ttcacgaaga tcgatacaac ggcgcggctg cagcgcgatt tcgccgcgcg tacgagggcc
     6601 gagtgccctt cgtggagggc gcccatggtc gggacaaggc ccaacgtctc gccggcgcgc
     6661 cgccatccgg cgacgatgcc gcgcagctcg ctgacggttc ttgcgacctg cacggtggcc
     6721 gatgtgccag ccgctgacgc tccagctccc gaggagccct tgcttgccat ctgcccctcc
     6781 gtccggtgca cggtcaattc tgcaccgcac cgcaaagtag tgctgcggcg tccccagcaa
     6841 caggggccat acgacgaatt tccgatgccg gggcgcgatc tgctattgtt ccgccgaacg
     6901 tcaggcgaat cgacccatca tgagtccccg gtcagaaccg tcaaaggcca agcgctcatg
     6961 tcacttgcac cacgccatgc cgtcggcgcg gcacaccccg ccaggggcgc gacttccgcc
     7021 ttgcctacgc gcgacgagat cagcacctat catctgcacg acgagacacg cctcgtcggc
     7081 gccctgatcg agcgcgcggt ctatacggag gacgagcgcc gccgcattgg cgagcatgcc
     7141 cgccgcctcg tcgaggcggc ccgcgcggac cgccggcgcc acggcggcct cgacgccttc
     7201 ctcgccgagt atggtctcac ctccgaggag ggcgtgatcc tcctctgcct cgccgaggcg
     7261 ctgctgcgca tcccggatac ccagacggcc gacgcgctca tcgccgagaa gatggccggc
     7321 ggccaatggg agcgccacct cggcgcctcc gacagcctgt tcgtcaatgc ctccaccttc
     7381 ggcctgatgc tgacaggcaa ggtcgtgcgc ctcaaggacg cgcgcggcat gaacccgttg
     7441 aacctcgtga aacgcatggt cgcgcgctcg ggcgagcccg tcatccgcca ggcgctccgg
     7501 caggcgatga agattctcgg cgaccaattc gtgctcggtc gcactatcgg cgaggcgatc
     7561 gaccgggcgc gctcctacga acggcgcggc tatcgcttct cgtacgacat gctcggcgag
     7621 gcggcgcgca cggcccgtga cgcggagcgc tatttcagcc gctacgtgac ggcgattgag
     7681 gcggtcggca aggcagcagg gccgctcgcc tcgctgcacg gcgatgcgct gatggcgcgt
     7741 ccaggcctct ccgtgaagct ctcggcggtc catccgcgct tcgagcccgg caaggaggag
     7801 cggctgcagc gtgagctcgt gccgcgcctc cgtgatctcg cccgccgcgc ccgggaggcc
     7861 ggactgccgc tcaccgtcga tgccgaggag caagaccgac tagacctgac gctcgatgcc
     7921 ttcgccgaag tgttcctcga tcccgtgctc gacggctggc cgggcctcgg cgtcgtcgtg
     7981 caggcctatg ggcgccgggc aattcccgta ttgcgatggc tgaagcgact ctccgacgtc
     8041 agcggcaagc gaattccggt gcgcctcgtc aagggcgcct actgggacag cgagatcaag
     8101 tgggcgcagg agcgcggttg gaccgactat cccgtgttca caaggaagct acacaccgac
     8161 gtctcctatc tggcggcgat gcgattcctc ctctccgatc cggtcgcctt ctttccgcaa
     8221 ttcgcgaccc acaatgccca ctcgatctcc tcgggcttga tcgcgggcgg caaccgcacg
     8281 ttcgagttcc agcggctgca cggcatgggc gaggcgctct acgaggctgt cgtcggcgac
     8341 ggcacggctg gccgcgtctg ccgcatctat gcccccgtcg gggggcatga ggacctgctc
     8401 gcctatcttg tccgtcgcct tctcgagaac ggcgccaaca cctcctttgt caatcgcctc
     8461 gcggacgagg atacgacggc cgccgagatg gtcgtcgatc ccgtcgaggc ggccgagcgc
     8521 gagtggcagt ccggccagct tggcgccgag cggacgctgc cgaagccgac ggagctcttt
     8581 gagccacgcc tcaacagcgt tggcctgccg ctctccgagc cgcgcgtgcg tgccgatgcc
     8641 gaaaaggcga tcgccaggga gctcgaggtg caaatcgctg ccggcccgat agtcgacggc
     8701 aaggtcacgg ccggcgggga cgaagcccgc ctcgccctct gcccgcacga ccggcgccag
     8761 cgcgtcggca cggtgcgcga ggcgaccccc gaggatatcg agcgctctat cgcggcctcg
     8821 ctgaaagccg cgcacgcctg ggatcgccgc ggcggtcacg agcgggcaaa gatcctcgag
     8881 gcggccgccg acctctacga gcgcaaccgc atgcgactca tggcagtgat cgtgcgcgag
     8941 gccggaaaga ccctcgagaa cgcgcaggcc gacatccgcg aggccgtcga tttcctgcgc
     9001 tactacgcgg ctgaggcgcg gcggctgttc gcggggcccg tgccgcttcc ggggccgacg
     9061 ggcgagcgca acacgctcga gcttcgcgcc cgcgggccga tcgcggcgat cgcgccctgg
     9121 aacttcccga t
//
LOCUS       DAORNH010000186         9074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5672_length_9074_cov_2.959641, whole genome shotgun sequence.
ACCESSION   DAORNH010000186 DAORNH010000000
VERSION     DAORNH010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5672_length_9074_cov_2.959641"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PK264_16920"
     CDS             complement(<1..577)
                     /locus_tag="PK264_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRD77591.1"
                     /translation="MFTLSELEAAAELVHGHVAPTPQHRWPLLARRTGVDVVVKHENH
                     TPCGAFKVRGGIVYMDELKRRQPDVPGVITATRGNHGQSIARAATAVGIPSTILVPHG
                     NSVEKNEAMRAFGAELIEFGADFDEAKEEAARLAKARGLHMVPSFDGLLVKGVATYGL
                     ELFRAAPDLDAVYVPIGMGSGTIAPLSFPLHL"
     gene            complement(595..2622)
                     /locus_tag="PK264_16925"
     CDS             complement(595..2622)
                     /locus_tag="PK264_16925"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HRD77592.1"
                     /translation="MGDKEASSVVSSEHVRPLWRPGPGVASATRLAAFLGEMREREPA
                     AAASVAELHRWSLREPERFWAAVWRFTGIVGAMGAEPYIDAKARVAEARFFPKGELNF
                     AENLLRPALQALVPGDRDALVFRGEDRVSRRLSWQALTAEVARGQAAWRASGIAAGDR
                     VAAILPNMPEAICGLLATASIGAVWSSCSPDFGVQGILDRFGQIEPRILIACDGYYYG
                     GKRIEMAEKLAAVLDGLPTVEQVIVVDYIGEAGAVASALSRSGGRRSATWADALSKRT
                     EQRPDFPRFPFNHPLYILFSSGTTGVPKCIVHSAGGTLLKHAAEHQLHTDVRAGDRLF
                     YFTTLGWMMWNWLVSGLSTGATLMLYDGSPFHPDGNVLWDYAAEERFTHFGTSAKYID
                     SLKKAGLAPARTHDLAALRAVMSTGSPLVAESFDYVYSDIKSDLHLASISGGTDIVGC
                     FVLGNPLAAVYRGEIQGAALGLDVAVYDGDGRPMTAGKGELVCRNPFPSRPIGFWNDP
                     DGRKYRKAYYERFDNTWHHGDFAEWTATGGMIIHGRSDATLNPGGVRIGTAEIYRQVE
                     QLPEVREAIVIGQDKDGDVRVVLFVVLEPGRSLDEALRQRIRSQIRTGASPRHVPARI
                     IQVPDIPRTKSGKITELAVRDIVMGRAIGNKEALANPEALEHFRNHPELKD"
     gene            2873..4555
                     /locus_tag="PK264_16930"
     CDS             2873..4555
                     /locus_tag="PK264_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRD77593.1"
                     /translation="MRALIPRARLMAVAIAIVGGLLLLIAAPLLFGPSSRLRLASVVA
                     SARDSMVITAPVRLSSTAPLVIDAGSIFSSDIPTAKSPVSGRMVIDGASIVVSQPEPG
                     PHASDDSVVPILSVLVGPGFDRLTIRRTEIRFSRSGRPGDLLANVSAEVTRRKQGLHA
                     KGKFTYLGQRLRFEATTGPIVADIKTKLRQPLRLVVTGAWFSAVFDGHLDIDGTVSLD
                     GQGEVSISDPRRAMRWLGLPIGSGESLRRTRAKGTVVWRNRTIAFQTVALAIDGNEAT
                     GALTVDFEGPRPLFDGTLAVKTLDLTPYIELEKSEPGMARRVEHHLPLARDIDIDMRL
                     SAATLIVGPHKFGGVAATVALKAGRLIADIAEMDFSEGTIGGQVLLDLAAMPTVLAVR
                     GRAQNFDLARISSALVGFAAGQGPATLTVDASARGATAIELLRSLSGKASLALSEGGR
                     LAVDVRHLIDADAPKDDLRRLWAAAKRGSTSFDKLEIKLSMRNGVVQSELVELASGPA
                     GVAVVGSYSLISDVLDLSIRRSKGASLAPAGKAASARVLGPSANPDIVRSPN"
     gene            4754..5245
                     /locus_tag="PK264_16935"
     CDS             4754..5245
                     /locus_tag="PK264_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRD77594.1"
                     /translation="MTYLAGLGIETRTVDHKAVFTVAQSKEIETGLPGGHIKNLFLKC
                     KKGQLHLVVALNSTAVDLKSLHKSLGSGRLSFGNAELLKATLGVEPGSVTPFALVNDT
                     AGIVAVALDARMMRHDLLNCHPLTNTATTAIARDDLMRFIRATGHEPRVIELSANALG
                     GAD"
     gene            5320..6231
                     /gene="trxA"
                     /locus_tag="PK264_16940"
     CDS             5320..6231
                     /gene="trxA"
                     /locus_tag="PK264_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRD77595.1"
                     /translation="MDFGKKPAPAGAGAGDLIKDTTTATFMKDVIEASREAIVLVDFW
                     APWCEPCKQLTPALERVVRGYGGKVRLVKMNIDQNKQIAAQLRIQSIPTVYAFRDGRP
                     LDMFQGALPESGLRQFIDRLVKEDSAGDIASVLAEAEAAFEAGDLQGAAEIFAAVLQE
                     DRQNVQALAGLARCYLKSGDIARAEQTIALVPPDQRSVAPISGVQAAIDLAKRAASAG
                     STSELEAKVAANPADHQARIDLAMAHAAAGDKLGAVGQLIESIKRDKAWGEQAARKQL
                     LQFFDAWGFKDPATVEGRRRLSSLLFS"
     gene            6268..6957
                     /locus_tag="PK264_16945"
     CDS             6268..6957
                     /locus_tag="PK264_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HRD77596.1"
                     /translation="MRIAERYRTIGDLPRQIPIFPLRNAILLPRAVLPLNIFEPRYLA
                     MVDFAIRSDRIIGMIQPSGAVSVTGSPRERDAKLWRVGCMGRITAFQEREDGRLLITL
                     TGIARFRPGAEVTSELPYRQVIVDSSDYADDLTPGIGANAVDRPRLLGVLKSYLEQKQ
                     LAADWSAIQQAESERLVNALSVLAPYSAEEKQALIEAKLLTERAELLIALAEMELAAA
                     RGGGGPGNIMQ"
     gene            6970..7188
                     /locus_tag="PK264_16950"
     CDS             6970..7188
                     /locus_tag="PK264_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRD77597.1"
                     /translation="MPEPDAERPDPKLLEILVCPLTRARLIYDRERQELVSLAARLAY
                     PIRHGVPQLVESEARALGDEEVARLARG"
     gene            7601..8809
                     /locus_tag="PK264_16955"
     CDS             7601..8809
                     /locus_tag="PK264_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD77598.1"
                     /translation="MRPTFHKDVRRLVDLNATNQGAAYWNFDTFITPVDDFFIRNEYA
                     TPRPESEPRVNPRTWRLKIHGDGIERPLEIGYEDLLKMPSRSIIATMECAGNGRTLFW
                     EQQGMLEAPTKVGGTGWGLGGIGQAEWQFVPMSHILGLVGLRREARWALFWSGVDNKE
                     GKPGVEGDTGRPLPVKALQRRGDDIGLAFKMNGNDLTPDHGAPVRAIVPGWCGAASTK
                     WLTEIKISRNNFWVRLNSKAHVLIGPSYSPPRPAPEDEFRHITEKDIRGVAVEWSPPK
                     SLITVPLVLEKQPKFPHNYPLKPGELPTLAAGRQTIKGYAWAPQFGVRRVEYRIDGGR
                     WQTATIREPNLGRYTWVRFDFDWAPAAGRHLVETRVTDNAKRTQPESVPYNEGGFDFW
                     AVPKFHINVV"
BASE COUNT         1564 a         3076 c         2962 g         1472 t
ORIGIN      
        1 agaggtgaag tggaaaggac aagggcgcga tggtgcccga acccatgccg atcggcacat
       61 agaccgcatc gagatcgggc gccgcgcgga acagctcgag gccataggtc gcaacaccct
      121 tgacgagcag cccgtcgaag gatggcacca tgtgcagtcc gcgcgccttg gcgagccgcg
      181 ctgcctcttc ctttgcctcg tcgaaatcgg cgccgaactc gatgagctcg gcgccgaacg
      241 cgcgcatcgc ctcgttcttc tcgacgctgt tgccgtgcgg gacgaggatc gtcgagggga
      301 taccgacggc ggtcgcagcg cgcgcgatcg actggccgtg attgccgcgc gtcgcggtga
      361 tgacgccggg aacatccggc tggcggcgtt tgagctcgtc catgtagacg atgccgccgc
      421 gcaccttgaa ggcgccgcag ggcgtatggt tctcgtgctt gacgacgacg tcaacgcccg
      481 tgcgccgcgc gagcagcggc cagcgatgct gcggcgtcgg cgcgacgtgg ccatgcacca
      541 gctcggctgc ggcctcgagc tcagagagtg taaacagcgg cttcggcatg gtccctaatc
      601 cttgagctcg ggatgattgc ggaaatgctc gagtgcctcg ggattggcga gcgcttcctt
      661 gttgccgatg gctcgcccca tgacgatgtc gcgtacggcg agctcggtga ttttccctga
      721 cttcgtgcgc gggatgtccg gcacctggat gatgcgcgcc ggcacgtggc gtggcgaggc
      781 gccggtgcgg atctgcgaac ggatgcgctg ccgcagcgcc tcgtcgagcg agcggccagg
      841 ctcgagcacg acgaacagca cgacacggac atcgccgtcc ttgtcctggc cgatgacgat
      901 ggcttcgcgc acctcgggaa gctgctcgac ctgacgatag atctcggccg tgccgatgcg
      961 cacgccgccg ggattgagtg tggcgtcgga gcggccgtgg atgatcatgc cgccggtcgc
     1021 cgtccattcg gcgaagtcgc catggtgcca ggtgttgtcg aagcgctcgt agtaggcctt
     1081 gcggtacttc cggccgtcgg gatcgttcca aaagccgatc gggcgcgaag ggaagggatt
     1141 gcggcaaacg agctcgccct tgcccgccgt catcggccgg ccgtcgccgt cgtaaacggc
     1201 gacgtcgagg ccaagcgctg cgccctggat ctcgccgcga tagacggctg ccagcggatt
     1261 gccgagcacg aagcagccga cgatgtccgt gccacccgag atggaagcga ggtgcaggtc
     1321 actcttaata tccgaataga cgtagtcgaa gctctcagcg accaggggcg aacccgtcga
     1381 catgacggcg cggagagccg caagatcgtg cgtgcgggcc ggcgcgaggc ctgccttctt
     1441 caggctgtcg atgtacttcg ccgaggtgcc gaaatgagtg aagcgctcct cggccgcata
     1501 gtcccagagc acgttgccgt ccgggtggaa gggagaaccg tcgtagagca tgagtgtcgc
     1561 gccggtcgag agccccgaga cgagccagtt ccacatcatc cagccgagcg tcgtgaagta
     1621 gaagagcctg tcgccggccc gcacgtcggt gtgcaattgg tgttcggccg cgtgcttgag
     1681 gagcgtgccg ccggccgaat gcacgatgca cttcggcacg cccgtcgtgc ctgacgagaa
     1741 caggatgtag agcgggtggt tgaaaggaaa gcgcgggaag tcggggcgct gctcggtccg
     1801 cttgcttagc gcgtcggccc aggtcgcaga gcgccggccg ccggatcgag acagcgcaga
     1861 ggccactgcg cccgcctcgc cgatgtagtc gacgacgatg acctgctcga ccgtcggcaa
     1921 tccatcgaga accgccgcga gcttctcggc catctcgatc cgcttgccgc cataatagta
     1981 gccgtcgcag gcgatgagga tgcgcggctc aatctgacca aagcggtcga ggatgccttg
     2041 cacgccgaag tccggcgagc acgacgacca gaccgcgccg atcgatgcgg ttgcgagcag
     2101 gccgcagatc gcttccggca tatttggcag gatggcggcg acgcgatcgc ccgccgcgat
     2161 gcctgatgcg cgccatgccg cctgcccgcg cgcgacctcg gccgtcagcg cctgccagct
     2221 caggcggcgg ctgacgcgat cctcgccgcg gaagacgagt gcatcgcggt cgccaggcac
     2281 gagcgcttgc agggccgggc gcaacaggtt ctcggcaaaa ttcaactcac ccttgggaaa
     2341 aaagcgcgcc tctgccacgc gtgccttggc atcgatgtac ggctcggcac ccatcgcgcc
     2401 gacgatacca gtgaagcgcc agacggcggc ccagaaccgc tccggttcgc gaagcgacca
     2461 gcgatgaagc tccgccacgc tcgcagccgc ggcaggctcc cgttccctca tctcgccgag
     2521 gaaggctgcg agccgcgtcg ctgaggcaac gcccggaccc ggtcgccaca acggccgaac
     2581 atgctccgag gagactaccg agcttgcctc tttgtcgccc atcggctcaa cctcgatccg
     2641 tcgtcagttt gccatgaggc cattgtctag tagcatcaca cgggtttgca cagcacggtg
     2701 agcggtgcaa gccttgtcca caaggacgtc gcttgcagac ggaagccggc tctggctata
     2761 gctcgatcga gatggcgtga tccagtatgg acggacgcga cggcgcgtgg gagccgttct
     2821 tgggctgatg gcccggagcc acaagttgga ccggagagga ggcgtcgcaa gaatgcgtgc
     2881 tttgatcccg cgagcgcgtc tgatggcggt ggcgatcgcc atcgtcggcg gcctgctcct
     2941 gctgattgca gcacccctct tgttcggtcc gtcgtcccgg ctgcgacttg caagcgtcgt
     3001 cgcctccgcc cgcgacagca tggtcatcac ggctccggtg cgactgtcgt cgacggcacc
     3061 actcgtgatc gacgcgggca gcatcttctc gagcgacatc ccgacggcaa agtcgccggt
     3121 atccggtcgc atggtgatcg acggagccag catcgtcgtc tcgcagcccg agcccggtcc
     3181 gcacgccagc gacgatagtg tagtcccgat cctcagcgtt ctcgtcggcc ctggcttcga
     3241 tcgcctgacc atccgccgca ccgagatccg cttctcgcgg tccggccgtc ccggcgatct
     3301 cctcgccaat gtgtccgccg aggtgacacg gcgcaagcag ggcctccacg ccaaaggcaa
     3361 gttcacctac ctcggccaga gacttcgctt cgaagcgacg accggtccga tcgtcgccga
     3421 catcaagacc aagctgcgcc agccgctgcg cctcgtcgtg acgggcgcat ggttcagcgc
     3481 ggtcttcgac ggccatctcg acatcgacgg caccgtctcg ctcgacggcc aaggcgaggt
     3541 cagcatcagc gatccgcgcc gagcgatgcg ctggctcggc ctgccgatcg gcagcggcga
     3601 aagcctcaga cgcacccgcg cgaaaggcac ggtcgtctgg aggaacagga cgattgcctt
     3661 ccagaccgtc gcactcgcca tcgacggcaa cgaggcaacc ggagcgctga cagtcgattt
     3721 cgaaggcccg cgcccactgt tcgacggcac gctcgcagtg aagacgctcg acctcacgcc
     3781 ctatatcgag ctcgagaagt ccgagccggg catggcccgc cgcgtcgagc atcacctccc
     3841 cctggcacgc gatatcgaca tcgacatgcg cctctcggcc gccacactga tcgtcgggcc
     3901 ccacaagttc ggcggcgtcg cggcgacggt ggcgctgaag gcaggccgcc tcattgcaga
     3961 catcgccgaa atggatttca gcgaaggcac gatcggtggc caggtgctgc tcgatctcgc
     4021 ggccatgccg accgtgctcg cggtgagggg gcgcgcccag aatttcgatc tcgcccgcat
     4081 cagcagcgcg ctcgtcggct tcgcggccgg ccagggtccg gccacgctga cggtcgatgc
     4141 cagcgcccgc ggtgcgaccg cgatcgagct cctgcgctcc ctgtcgggca aggcgtcgtt
     4201 ggcgctcagc gaaggcggcc gtcttgccgt cgatgtccgt catctcatcg atgccgatgc
     4261 gccgaaggac gatctgcgcc ggctgtgggc cgcggccaag cgtggctcca cctcgttcga
     4321 caagctcgag atcaagctgt cgatgcgcaa tggcgtcgtt cagagcgagc ttgtcgagct
     4381 cgcgtcgggt ccggcaggcg tcgccgtggt cggcagctac agtctcatca gcgacgtgct
     4441 cgacctcagc atccgcagga gcaaaggggc atctctggct ccggccggca aggccgcgag
     4501 tgcgcgcgtc ctcggaccga gcgccaatcc ggacatcgtc cgctcgccga actgagcccg
     4561 gcccccggct cgacctgttg cagctcatgt gccgactggc gctgaccgac gatcgcttgt
     4621 cgccggcgct tgctgcaggg ctcggtccgc tctatctcat cggcaaacct cctccgatag
     4681 ccttcctcac gcggaaccaa tccccatgcc gccggccgct ccaacgcccg cccaactcga
     4741 tcgcgcgggc ctgatgacct acctcgcagg ccttggcatc gagacgcgga ccgtcgatca
     4801 caaggccgtc ttcacggtcg cgcagagcaa ggagatcgag accgggcttc ccggcggaca
     4861 catcaagaac ttgttcctga agtgcaagaa ggggcagctc cacctcgtcg tcgcgctcaa
     4921 ttcgaccgcg gtcgatctca aatccttgca caagtcgctc ggctccggcc ggctgagctt
     4981 cggcaacgcg gaactcctca aggcgaccct cggtgtcgag ccgggctccg tgacgccctt
     5041 cgccctcgtt aacgacacgg cgggcatcgt cgccgttgca ctcgatgcgc gcatgatgcg
     5101 gcacgacctc ctcaactgcc acccgttgac caacaccgcg acgactgcca ttgctcgcga
     5161 tgatctgatg cggtttatcc gggccaccgg acacgagccg cgcgtcatcg agctctccgc
     5221 caatgccctg ggcggtgccg attgacccgt gcagccctgg cgacgcgagt ggcccctcgg
     5281 ccctcgggcg gtcgctcagc agaactcgag gagtagtgca tggacttcgg taagaagccc
     5341 gcaccggccg gcgccggcgc cggtgacctg atcaaggaca cgaccaccgc gaccttcatg
     5401 aaggacgtga tcgaggcatc gcgcgaagcg atcgtactgg tcgatttctg ggcgccttgg
     5461 tgcgagccct gcaagcaact gacgccggcg ctcgagcgcg tcgtgcgcgg ctacggcggc
     5521 aaggtgcggc tggtcaagat gaacatcgac cagaacaagc agatcgccgc gcagctgcgc
     5581 atccagagca tcccgaccgt ctacgccttc cgtgatggcc gccctctcga catgttccag
     5641 ggcgcactcc ccgaaagcgg gctccgccag ttcatcgacc ggctcgtcaa ggaggatagt
     5701 gccggcgaca tcgcctccgt gctcgccgag gcggaggccg ccttcgaggc gggggacctg
     5761 cagggtgcgg ccgagatctt cgccgcagtc ctccaggagg atcgccagaa cgtccaggcg
     5821 ctcgccggtc ttgcgcgctg ttacctgaag tcgggcgaca tcgcccgtgc cgagcagacc
     5881 attgcgctcg taccgccgga tcagcgctct gtcgcgccga tctcgggtgt tcaagccgcc
     5941 atcgacctcg ccaagagggc ggcgagcgca ggctccacct ccgagctcga agccaaggtc
     6001 gccgccaatc cggccgacca ccaggcgcgc atcgatctcg ccatggcgca tgccgccgcc
     6061 ggcgacaagc tcggcgccgt cggccagctg atcgagagca tcaagcgcga caaggcatgg
     6121 ggcgagcagg cagcgcgcaa gcagcttctc cagttcttcg atgcctgggg cttcaaggat
     6181 cccgccaccg tcgagggccg ccgccgcctc tcctcgctgc tgttctcttg agcagaaccg
     6241 ggccaccgac acgagagcga gaccggcatg cggatcgcag agcgctatcg aaccatcggc
     6301 gatttgccac ggcagatccc gatctttccc ttgcgaaatg cgatcctgct gccgcgcgcc
     6361 gtgctgcccc tcaacatatt cgagccgcgc tatctcgcca tggtcgattt cgcgatccgc
     6421 tcggatcgca tcatcggcat gatacaaccg tcaggcgccg tcagcgtgac cggatcgccg
     6481 cgtgagcgag acgcgaaact ctggcgcgtc ggctgcatgg gccgcatcac cgccttccag
     6541 gagcgcgagg atggccgcct cctcatcaca ctcaccggga tcgcccggtt tcggccggga
     6601 gccgaggtca cgagcgagct tccctaccgc caggtcatcg tcgacagctc cgactatgcc
     6661 gacgacctca cgccagggat cggtgcaaac gcggtggacc ggccccgcct tctcggtgtg
     6721 ctcaagtcct atctcgagca gaagcagctc gccgccgact ggagcgcgat ccagcaggcg
     6781 gagagcgagc gcctggtcaa tgccctctcc gtgctcgcgc cctacagcgc tgaagagaaa
     6841 caggcgctga tcgaggcgaa gctgctcacc gagcgcgcgg agctcctgat cgcgctcgcc
     6901 gagatggagc tcgcggccgc gcgtggcggt ggcggccccg gcaatatcat gcagtaagcg
     6961 agagagccga tgcccgagcc cgacgcggag cgccccgacc cgaagcttct cgagatcctg
     7021 gtttgcccgc tgacgcgcgc ccgcctcatc tacgatcgcg agcgccagga gctcgtgagc
     7081 ctcgccgcgc gcctcgccta tcccattcgc cacggcgtgc cgcaactcgt ggagagcgaa
     7141 gcgcgggctc tcggcgacga ggaggtcgca cgcctcgccc gcggctgagg ttcgcgcgtg
     7201 gcaagccgat gccgaaccgc gccagcggaa ccggcagcac acagatcaca tttgtgtgaa
     7261 agctaccaca cacgcggaaa ttgcgacgcg ccgctcagcc tccgtgcgca aaggagttgt
     7321 tcgcgcttag tcgcatgcta gtttttcggc attgttttgc ggctgggggg ccaaaatgtc
     7381 gagtagcagt aacagcaaga gtacgaatgc gcgtgagcgt cgcctgcttc aacgtctgat
     7441 ggcagatcgg acgatcgatc ggcggaattt tttcatctca gcagccgcgt tcggagctgg
     7501 tgtggctgca tccggcccga tcgggactgc tggcgcccag caggttgcag cggccgcccg
     7561 cctgcctatt gcgaggacgg acccgaggcc tgaggcgcgc ttgcgcccca cattccacaa
     7621 ggatgtgcga cggctcgtcg acctcaatgc caccaatcag ggtgctgcct actggaactt
     7681 cgacaccttc atcacgccgg tcgacgactt cttcatccgc aacgagtacg cgacgccacg
     7741 ccccgagagc gagccccgcg tcaacccgcg cacctggcgc ctgaagatcc acggtgacgg
     7801 catcgagcga ccgctcgaga tcggctacga ggatctcctc aagatgccgt cgcgctccat
     7861 catcgcgacg atggagtgcg ccggtaacgg acgcacgctg ttttgggagc agcaaggcat
     7921 gctcgaggcg ccgacgaagg tcggcggcac cggctggggc ctcggcggta tcgggcaggc
     7981 cgaatggcaa tttgtgccga tgagtcatat tctgggcctt gtcggtctcc ggagggaggc
     8041 gcgatgggcg ctcttctggt ccggcgtcga caacaaggag ggcaagccgg gggtcgaggg
     8101 cgacacgggg cgcccgctcc ccgtgaaggc gctgcagcgg cgcggcgacg acatcggcct
     8161 tgccttcaag atgaacggca acgacctcac gcccgatcac ggcgcgccgg tccgcgcgat
     8221 cgttccaggc tggtgcggcg ccgcctccac gaagtggctg accgaaatca agatctcgcg
     8281 caacaatttc tgggtccgcc tcaactccaa ggcgcatgtg ctgatcggcc cgagctacag
     8341 cccgccgcgt cctgctcccg aggacgagtt ccgtcatatc acggagaagg acatccgtgg
     8401 cgtcgccgtc gaatggtcgc cgccgaagtc gctgatcacg gtgccgcttg tcctcgagaa
     8461 gcagccgaag ttcccgcaca attacccctt gaagccgggt gagctgccga ccctcgcggc
     8521 cggtcgacag acgatcaagg gctacgcctg ggcgccgcag ttcggcgtcc gccgtgtcga
     8581 gtatcgcatc gatggcgggc gctggcagac cgcgaccatc cgcgaaccga acctcgggcg
     8641 ctatacttgg gtacgcttcg acttcgactg ggcaccggcg gcgggccgtc atctggtcga
     8701 gacgcgcgta accgacaatg ccaagcgcac ccagccggag agcgtgccct ataacgaggg
     8761 cggattcgac ttctgggccg tcccaaaatt ccatatcaac gtggtctgac ggccggaggc
     8821 ggtgagcttc ggtctgcgag aatagggggc acatcccgat gcggggaaaa tttgctttgg
     8881 agccgtccac gacggcgccg ggccgatcca tgtcgcgccg cgcgctgctc agcggcatgg
     8941 gtacggccat cggcgccgcg acgtcgctgc atgcgggatc agcccgcgcc gacaaccccg
     9001 tcgcagggtc ggcgaccgga gaccggccga tggcgcggat gaggtcggcc gcccgttcgg
     9061 tcagcgcggc ttcc
//
LOCUS       DAORNH010000187         9068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5676_length_9068_cov_2.683457, whole genome shotgun sequence.
ACCESSION   DAORNH010000187 DAORNH010000000
VERSION     DAORNH010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5676_length_9068_cov_2.683457"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PK264_16960"
     CDS             <1..283
                     /locus_tag="PK264_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HRD77599.1"
                     /translation="HANAFLLADKLAAVSGVEVLTPAFFNEFTIRVRGDAGAVIEKLA
                     AKGVLGGIPVSRLIPGKAQLGDLIVVAATEINTDDDRTAFAKAIGEVLA"
     gene            280..564
                     /locus_tag="PK264_16965"
     CDS             280..564
                     /locus_tag="PK264_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77600.1"
                     /translation="MSYQAAIMARIAAIDPKTLPVSAKMAWASHVARTVRQPADQRLG
                     LGDVRMLEAWPRERLAKLEAALDDIDRIVDRDRNERWNDDVRVKVARTYL"
     gene            594..2162
                     /gene="gcvPB"
                     /locus_tag="PK264_16970"
     CDS             594..2162
                     /gene="gcvPB"
                     /locus_tag="PK264_16970"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRD77601.1"
                     /translation="MTMNTQGRPTGPGAASRSAVETYSGHRGLDHEEPLLFELGAPEK
                     TGVDLVEPKSGKSRLGGLERAEPIGLPGLTEPETVRHYVRLSRKNYAIDSGVYPLGSC
                     TMKHNPRLNEKMARLAGFSDIHPLQPVSTVQGAIALMEMLAGYLMELTGMKAVTMSPK
                     AGAHGELAGMLAIKAAHEAKGSKRSVVLVPDSAHGTNPATAAFMGYSVRSIPAKDDGT
                     VAVEAVKAALGPDIAAIMLTNPNTCGLFEPEIAAIADAVHASGAYFYCDGANFNAIVG
                     KVRPGDLGIDAMHINLHKTFSTPHGGGGPGSGPVVLSAALAPFAPLPYAVRSGDQFRL
                     VEDSSQADERSPFGRLTAFHGQMGMFVRALTYMLSHGADGMRQASEDAVLSANYIRAS
                     LSDVMSQPFGDKPCMHEVLFDDAWLKDTGVTTLDFAKAMIDEGYHPMTMYFPLVVHGA
                     MLIEPTESESKFALDQFIGSLRGLASAAKSGNDKSRFSNAPLHAPRRRLDETKAAREP
                     RLRWRPPQAKQAAE"
     gene            2166..2465
                     /locus_tag="PK264_16975"
     CDS             2166..2465
                     /locus_tag="PK264_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77602.1"
                     /translation="MLFMVISTPRPERPSERAADRTRYWPWLAAHERAGRCKGAWAKV
                     GRGAVVLLDVATHDELHAILNEWADIIPATFEVLPLIDTSAAKGFLAGKAGSSPS"
     gene            2495..2881
                     /locus_tag="PK264_16980"
     CDS             2495..2881
                     /locus_tag="PK264_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD77603.1"
                     /translation="MPRKLISSGSRFEAEVGYSRAVVDGDMIFVSGTTGYDYKAMSIA
                     PEVEAQCVQTFENIKAALALAESSLDDVVRATFILPDRADWPKCWPIVSRYFGKVRPA
                     STMIVAGLAEPEMRIEIEVTAKRSRG"
     gene            2878..3924
                     /gene="epmA"
                     /locus_tag="PK264_16985"
     CDS             2878..3924
                     /gene="epmA"
                     /locus_tag="PK264_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HRD77604.1"
                     /translation="MSGPSPWWSPSRHEDRRPRLILRNRIKAAFRAWFEGEGFTEVET
                     AALQVSPGNEAHLHAFATSMIGDDGRAVPRYLHTSPEFACKKLIAAGETRIFTFAPVY
                     RNRERGPLHHPEFAMLEWYRVGAPYHRLIEDCAELMRRAALITGSAQWQFREATADAA
                     AQPVRVTVADAFERFAGIHLLEDGIGANGSVDRDRFAAAASARSIRVAADDTWSDVFS
                     RVLVERVEPGLRGMSAPVVLDEYPAHEAALARRVPRDPRVSERFELYACGVELANAFG
                     ELTDAGEQRRRFEAEMDERERVWGERYPIDDALLAALGTMPPTSGIALGFDRLVMLAT
                     GASRVDDVIWTPFE"
     gene            4307..5365
                     /locus_tag="PK264_16990"
     CDS             4307..5365
                     /locus_tag="PK264_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRD77605.1"
                     /translation="MSSKRWVKRPDDSTWGDYGADDQLGRMNELTPLKVKQGIAEVKE
                     GLTFCLSMPLDYPGGSILNPRRHPPQIRPTLRNNKPNFIYQVAWDHPGMTDVINDDMV
                     LLHLQYSTQWDTLAHVGALFDADGDGKPEPRFYNGYQGGVHIVGPIEGKDAGAVPGTF
                     VESKGTSSANALGVENMAVKCLQGRGVMIDLFAHFGRQRKAVGYDDLMRIMAADGIKV
                     EAGDFVLLRTGFDDVILEMKKKPDAHILENICTGLDGSDQRLLKWVTDSRLVALISDN
                     YAVEIHNAPLKPGQCAMLPLHQHCLFKTGVNLGEIWRLSELADWLRKNNRSRFLLTAP
                     PLRLPGAVGSPATPVATV"
     assembly_gap    5456..5575
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5576..6305)
                     /locus_tag="PK264_16995"
     CDS             complement(<5576..6305)
                     /locus_tag="PK264_16995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD77606.1"
                     /translation="MPSIRQLEYLVALAETRNFRRAAERTNTTQPTLSEQIKALEDRL
                     GAQLVERSRSGTMLTPIGAQVVEISRRILKDSNDIKTVAAAGDSSLKGVLKLGTAATV
                     GPYLLPLVVTSLHRAYPDLKLYVREQMPDSLPRALEEGSHDFIISPLPLRGDDLVSVE
                     LLREPLFLTVAADHPLATKERVERADLLDLEVLTLGPGHQLHDVVIALCEEFGARLRL
                     DYEGTSLDMVREMVITGLGVTFMPG"
     gene            complement(6478..8300)
                     /locus_tag="PK264_17000"
                     /pseudo
     CDS             complement(6478..8300)
                     /locus_tag="PK264_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8307..>9068)
                     /locus_tag="PK264_17005"
     CDS             complement(8307..>9068)
                     /locus_tag="PK264_17005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRD77607.1"
                     /translation="PLSPTGARIPPPVGAGRTPNVEADAAHGKGWFEVQDEGSQIAAQ
                     LAGAGPRHQVLDLCAGAGGKTLAMAARMQNNGQIYAYDRDKAQLRPIFERLKRAGARN
                     VQVLAGGDKAALTALGPRFDVVLADAPCTGSGVWRRRPESKWRVKPQSIAERQAEQRA
                     VLALGAALVKPGGRFVYVTCSVLPEENTDQVAAFIAERPEFTVVPTAKAWAAAMPGVD
                     CPASADGSADTLLLSPASHGTDGFFIAVLEKAKAT"
BASE COUNT         1478 a         2979 c         2978 g         1513 t
ORIGIN      
        1 tcacgccaac gccttcctgc tcgccgacaa gctggcagca gtgagcggcg tcgaggtgct
       61 gacgcccgcc ttcttcaacg agttcacgat ccgcgtgcgc ggcgatgctg gcgccgtcat
      121 cgagaagctc gcggccaagg gtgtgctcgg cggcattcca gtctcacggc tcatcccggg
      181 gaaggcgcag ctcggcgacc tcatcgtcgt cgccgcgacc gaaatcaaca ccgacgacga
      241 ccgcaccgcc tttgcgaagg caatcgggga ggtgcttgca tgagttacca ggcggcgatc
      301 atggcgcgta tcgcggcgat cgacccgaag acgttgccag tgtcggcgaa gatggcctgg
      361 gcaagtcatg tcgctcggac cgtccgccag ccggccgacc agcggctcgg cctcggcgac
      421 gtccgcatgc tcgaggcctg gccacgcgaa cgcctcgcca agctcgaagc ggctctcgac
      481 gacatcgatc gcatcgtcga tcgcgatcgc aatgagcgct ggaacgacga cgtgcgcgtc
      541 aaggtcgcgc ggacctatct ctgaccgaca ttcaagaggc ttggatcacg accatgacca
      601 tgaacaccca agggcggccc accggccctg gagccgcaag ccggagcgcc gtcgagacat
      661 actccggtca ccgtggcctc gaccacgagg agccgctgct cttcgagctc ggggcgcctg
      721 aaaagacggg cgtcgacctc gtcgagccaa aatcggggaa gagccgcctc ggcgggctgg
      781 agcgcgccga gccgatcggt ttaccgggac tgacggagcc ggagacggtc cgtcactacg
      841 tgcggctttc ccgcaagaac tatgcgatcg acagtggcgt ttatccgctc ggatcgtgca
      901 cgatgaagca caatccgcgc ctcaacgaga agatggcccg cctcgccggc ttctcggaca
      961 tccaccccct gcaacccgtg tcgaccgtgc agggtgccat agcgctcatg gagatgctcg
     1021 ccggctacct catggagctg accggcatga aggccgtgac gatgtcaccg aaagcgggcg
     1081 cccacggcga acttgccggc atgctcgcga tcaaggccgc gcacgaggcg aaaggcagca
     1141 agcgcagtgt cgtgctggta ccggattcgg cccacggcac gaatccggcg acggccgcct
     1201 tcatgggcta ctcggttcgc tccatcccgg cgaaggatga cggcaccgtc gcagtcgaag
     1261 ccgtgaaggc ggcgctcggg ccggacatcg cggcgatcat gctgacgaac ccgaacacgt
     1321 gtgggctctt cgagcctgag atcgcggcga tcgccgatgc cgtgcacgcg tccggtgcct
     1381 acttctattg cgacggcgcc aacttcaacg ccatcgtcgg caaggtgcgg cccggcgacc
     1441 tcggcatcga tgccatgcac atcaacctgc acaagacgtt ttcgacgccg cacggaggcg
     1501 gcggaccggg atcgggtccg gtcgtgctgt cggcggccct tgccccgttc gctccgcttc
     1561 cctatgcggt gcggtcgggc gaccagttcc gcctggtcga ggacagctcg caggcagacg
     1621 agcgctcgcc cttcggccgc ctcacggcct tccacggcca gatgggcatg ttcgtccgcg
     1681 cactcaccta tatgctgtcg cacggcgcag acggcatgcg gcaggcctcg gaggatgcag
     1741 tgctctcggc caactacatc cgcgcctcgc tcagcgacgt catgagccaa cccttcggcg
     1801 acaagccttg catgcacgag gtgctgttcg acgatgcctg gctgaaggac acgggtgtca
     1861 ccacgctcga cttcgcgaag gccatgatcg acgagggata tcatccgatg acgatgtact
     1921 tccccctcgt cgtgcacggt gcgatgctga tcgagccgac cgagagcgag agcaagttcg
     1981 cgctcgatca gttcatcggc tcgctgcgcg gtctcgcttc ggcagcaaag agcgggaacg
     2041 acaagagccg cttcagcaac gcgccgctgc acgcgccgcg ccgtcgcctc gacgagacga
     2101 aggctgcgcg cgaaccgcgg ctgcgctggc gtccccctca agccaagcag gctgcggagt
     2161 gagccatgct cttcatggtg atctcgacgc cgcggcccga gcgtccatcc gagcgggccg
     2221 ccgatcgcac ccgctactgg ccctggcttg cagcgcatga aagggccggg cgctgcaaag
     2281 gcgcttgggc aaaggtcggt cgcggtgccg tcgtgctcct cgatgtggca acgcatgacg
     2341 agttgcacgc gatcctgaac gagtgggcgg acatcatccc ggcgaccttc gaggtgctgc
     2401 cgctgattga cacgtcggcg gcgaagggct ttctcgcagg caaggccggc agcagtccga
     2461 gctgacgccc gatgccaaat ctggagactg cagcatgccc cgcaagctca tctcgtccgg
     2521 ctcccgtttc gaggcggagg tcggctattc gcgcgccgtt gtcgacggcg acatgatctt
     2581 cgtctccggc accacgggct atgactacaa agcgatgtcg atcgcgcccg aagtcgaagc
     2641 ccagtgcgtg cagaccttcg agaacatcaa ggcggcgctc gccctcgccg agagctcgct
     2701 cgacgatgtc gtccgtgcga cgttcatcct tcccgaccgg gcggactggc cgaagtgctg
     2761 gccgatcgtc tcgcgctact tcggcaaggt gcgaccggct tcgaccatga tcgtcgcggg
     2821 cctcgccgag cccgagatgc gcatcgagat cgaggtgacg gcgaagcgat cgcgcggatg
     2881 agcgggccgt cgccctggtg gtcgccgagc cggcacgagg accgccggcc gcggctcatc
     2941 ctgcgcaacc gcatcaaggc ggcctttcgc gcctggttcg agggcgaagg cttcaccgag
     3001 gtcgagacgg cagccctgca ggtgtcgccc ggaaacgagg cgcatctcca cgccttcgcc
     3061 acctcgatga tcggcgacga tggccgcgcc gtgccgcgct acctccacac ctcccccgag
     3121 ttcgcctgca agaagctgat cgccgccgga gagacgagga ttttcacgtt cgccccggtc
     3181 taccgcaatc gcgagcgcgg gccgctgcat caccccgagt tcgcgatgct cgagtggtat
     3241 cgggtcggag cgccgtatca tcggctgatc gaggactgtg ccgagctgat gaggagggcc
     3301 gcgctgatca ccgggtcggc gcagtggcaa tttcgggagg cgactgctga tgcggcggcg
     3361 cagccagtcc gcgtgacggt cgccgatgcc tttgagaggt tcgcgggcat ccacctcctt
     3421 gaggacggca taggtgccaa cggcagcgtc gaccgggatc gcttcgcggc ggcagcctcg
     3481 gcacgcagca tccgcgttgc tgccgacgat acctggtccg atgttttcag ccgcgtcctc
     3541 gtcgagcgcg tcgagcccgg tcttcgcggc atgagcgcac ccgtggtgct cgacgagtat
     3601 ccggctcacg aggcagcact cgcgcgccgt gttccccgcg accctcgcgt ctccgagcgc
     3661 ttcgagctct atgcgtgcgg tgtcgagctc gcaaacgcgt tcggcgagct cactgatgcg
     3721 ggcgagcagc gccgccgctt cgaggcagag atggacgagc gcgagcgggt gtggggggag
     3781 cgatatccga tcgatgacgc gctcctcgcg gctctcggga ccatgccgcc gacatcgggc
     3841 atcgccctcg gcttcgatcg gttggtcatg ctggcgacgg gcgcatcgcg cgtcgatgat
     3901 gtgatctgga caccgttcga atagtcagtc cgctgctttt gcgtgcaaaa tgtgcatctc
     3961 cagaatgcac gatcgcggat ttgtgaatta tcgtgacacg agatgcccgc tcgtgtatca
     4021 accctgtcac aaataagggc cggccgctca gagccatttc gcccaagtct agggatgggt
     4081 acgggtcagg cgtacagaaa ttgtcctgag ggcgctcaat tgaaggcgct gatggcagct
     4141 tccggatgat ctcgggcgag gcttcagagc ctcgcccgat ttgtttttgg gtccctctat
     4201 ttcgcgctcc atgagtgcgt cgcccgcttg tcggcgccgg acccacgtac ttgcgctcgc
     4261 cgatccgagg cctatgctcg aggcgctcga caatcgggag gtggagatga gctcgaagcg
     4321 ctgggtgaag cgaccggacg attcgacctg gggtgactac ggggcggacg atcagctcgg
     4381 ccgcatgaac gagctgacac ctctcaaggt caagcagggc atcgccgagg tgaaggaggg
     4441 cctcaccttc tgcctctcca tgccgctcga ctaccccggc ggttcgatcc tcaatccgcg
     4501 ccgccatccg ccgcagatcc gcccgacctt gcgcaacaat aagccgaact tcatctacca
     4561 ggtcgcctgg gaccatcccg gcatgaccga cgtcatcaac gacgacatgg tgctgctgca
     4621 cctccagtac tcgacgcagt gggatacgct tgcccacgtc ggcgcgctct tcgatgcgga
     4681 cggtgacggc aagcccgagc cgcgcttcta caacgggtat cagggcggcg tgcacatcgt
     4741 cggcccgatc gagggcaagg acgctggcgc cgtccccggc accttcgtcg agagcaaggg
     4801 cacctcgtcg gccaatgcgc tcggcgtcga gaacatggcg gtcaaatgcc tgcagggccg
     4861 cggcgtgatg atcgacctgt ttgcccactt tgggcggcag agaaaggcgg tcggctacga
     4921 tgatctcatg cggatcatgg cagcggacgg catcaaggtg gaagcgggcg acttcgtgct
     4981 gctgagaacg ggattcgacg acgtcatcct cgagatgaag aaaaagcccg atgcccacat
     5041 tctcgagaac atctgcacgg gtctcgacgg ctcggatcag cggctcctga agtgggtgac
     5101 cgacagcagg ctcgtcgccc tcatctcgga caactacgcc gtcgaaattc acaacgcgcc
     5161 cttgaagccc ggccagtgcg caatgcttcc cctgcaccag cattgcctct tcaagacggg
     5221 cgtcaatctc ggcgagatct ggcgcctctc cgaacttgcc gactggctcc ggaagaacaa
     5281 ccggagccgg ttcctgctga ccgcgccgcc cctcaggctg ccgggcgccg ttggctcgcc
     5341 ggcaacgccc gtcgccacgg tctgagcgac cacccgagac cgcctgacgc ctcggcgtca
     5401 ggtgatgcgg ttcagttcca cgacctcctt gcgtgcgatg tcgcggaaga ggctcnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcccc
     5581 ggcatgaagg tgacgccgag cccggtgatc accatctcgc gcaccatgtc gagggaggta
     5641 ccctcgtagt ccagccgcaa gcgtgcaccg aactcctcgc agagcgcgat gacgacgtcg
     5701 tgcagctggt ggccaggtcc gagggtcagg acctccaggt ccagcagatc ggctcgctcg
     5761 acgcgctcct tggtggcgag cggatggtcg gccgcgacgg tcaggaacag cggctcacgc
     5821 aggagctcga ccgagaccaa gtcgtcgccg cgtaatggga gcggcgagat gatgaagtcg
     5881 tggctgcctt cctcgagggc tcgaggcagg ctgtccggca tctgctcacg gacgtagagc
     5941 ttgagatcgg gataggcgcg atggagcgat gtgacgacca gcggcagcag gtaggggccg
     6001 acggtcgcgg ccgtgccgag cttcaggacg cctttgaggc tcgaatcgcc ggccgcggcc
     6061 actgtcttga tgtcgttcga gtctttcagg atgcgccggc tgatctcgac gacctgcgcg
     6121 ccgatcggcg tcagcatcgt gccggagcgg ctgcgctcga cgagctgcgc gccgagccga
     6181 tcctccagcg ccttgatctg ctcgctcagc gttggctgcg tcgtgttggt ccgctccgcg
     6241 gcacgtcgga aattacgcgt ttcggctaaa gctacgagat attcgagttg gcgaatactt
     6301 ggcatctggc gcctccgtgt ttagctccga ccatggtccg tgagctgcca tgaccgatag
     6361 atgcgcccta tgggacgctc tgcccagacg acatcgcata ttccgatcaa tagacagcgg
     6421 cggggcgatg ggaggacctg cttcggcagc cccgatggcg tgcgccctgc cacctggtca
     6481 gccgcccttg ccctcgaggt cctcgcgcag aatctcaagc cgcagccagt cctcttcggc
     6541 cgcggcgagt gcggtcgcgg cctcggccag gcgccgcgtc gccgcatcga agcgcttcgg
     6601 gtcgcgctga tagaggcccg gcgccgcaat ctccgcctcg agcttgccga tctccgcggt
     6661 gagcttgtcc atggtcgccg gcagggtctc gagcgcgtgc ttgtccttga aggagagctt
     6721 gcgcgcgcgt ggcgcctgaa tggcgggggc cggcgtcgta atgtcgaggg ctcgcgcatc
     6781 tcgccggcgc gccccaccat ccgacggcag gtcccggcca cgcctctgcg cgagcatgtc
     6841 attgtagccg cccgcatact cggaccagcg cccgtccccc tcggccgcca gcaccgacgt
     6901 cactactcga tcgaggaaat cgcggtcgtg actgacgagc agcaccgtac cctggtaggc
     6961 ggcaagcatc tcctgaagga gatcaagcgt ctcgaggtcg aggtcgttcg tcggctcgtc
     7021 gaggacaagc agattcgacg gcctggcgag cgcgcgcgcg agcatcagtc gaccgcgctc
     7081 gccacccgaa agggccgaca aaggcgttcg cgcctgttcg ggcttgaaca ggaagtcctt
     7141 catatagctc gcgacgtgcc ggccaatgcc gttgatgacg acgctgtcgc cgcgcccccc
     7201 ggtcagcgcg tcggtgaccg tccaggccgg cttcagctcg tcgcgtttct ggtcgagcgt
     7261 gaccatctcg atgcccgtgc cgaggcggag cgtgccttcg tcgggcgcca gctcgccgac
     7321 cagcatcttg atgagcgacg tcttgccggc gccgttcggg ccgacgatgc cgatgcggtc
     7381 gcctcgcatg acccggagcg agagatcatg cacgatccgc gtgtcgtcga aactcttact
     7441 gatccgcgtc gcctcgatga ccaggcggcc ggattgtccg ccctcggccg cggccatcac
     7501 gacattgccg tcgctgttgc gccactcgcc ttgcggcgca gctcgtgcat ttcggcaacg
     7561 cggcgcacgt tgcgcttgcg gcgcgccgtg acgccgccgt gcatccattc ctgctcgcgg
     7621 gcgatcttgc ggtcgagctt gtggcgctcg tcggcctcgc ggtcgagctc gccgtcgcgc
     7681 cattcctcga aggaggcgaa gccttggtcg atccgtcgtg tgctgccgcg gtcgagccag
     7741 acgatggagc gcgacagcgt ctcgaggaag cggcggtcat ggctgatgac gacgagcgcg
     7801 cagttctggc tcgcgacctc cttctcgagc cattcgatcg ccggaaggtc gagatggttc
     7861 gtcggctcgt cgaggaggag aatgtcgggg cggggggcca gcgcccgggc tatcgccgca
     7921 cgtcgcgcct cgccgccgga aagcgttgcc ggttcctctt cgcccgagag gccaagatga
     7981 tcgagcaggc ggcgtgcgcg gctctggtcc cccatcggtc cgaggcctgc ctcgacgtag
     8041 gcaagcgtcg aggtgaagcc ggagaggtct gcttcctgcg gcaggtagcg gagcacggcg
     8101 ctcggcgcga ggaagcgctg gcccttgtcg ggctccacgg tgccggcggc tatgcgcatc
     8161 agtgtcgact tgcccgagcc gttgcggccg acgaggcaga tgcgatcgcc gggagcgaca
     8221 agcagcgatg cgccctccag cagcggcgtg ccgccgaacg tcaagtgcac atcgtcgagg
     8281 gtcagcagcg gtggtgccat ggcgggctac gtcgcctttg ctttctcgag gacggcaatg
     8341 aaaaagccgt cggtgccatg cgaggcgggc gagagaagca gcgtatcggc gctgccatcg
     8401 gcggacgcgg gacagtcgac gcccggcatg gccgcggccc acgccttcgc agtcggcaca
     8461 accgtgaact cgggccgctc cgcgatgaag gccgccacct ggtcggtgtt ctcctcgggc
     8521 agtaccgagc aggtcacgta gacgaagcgg ccaccgggct tgacgagggc ggcgccgagc
     8581 gcgagcacgg cgcgctgctc cgcctgccgc tcggcgatgc tctgtggctt cacgcgccac
     8641 ttcgattcgg gccgccgtcg ccagacgcct gagccggtgc acggcgcatc ggcaagaacg
     8701 acgtcgaaac ggggcccgag tgccgtgagt gccgccttgt cgccgccagc cagcacctgg
     8761 acattgcggg cgccggcgcg cttcaaacgt tcgaagatcg gcctgagctg agccttgtcg
     8821 cgatcgtagg catagatttg gccgttgttc tgcatgcgcg cggccatggc gagcgtcttg
     8881 ccgccggcac cggcgcacag gtcaagcact tggtgtcgcg gaccggcacc ggcgagctgc
     8941 gctgcgatct ggctgccttc gtcctgcacc tcgaaccagc ccttgccgtg cgcggcgtcc
     9001 gcttccacat ttggcgtgcg gcccgcgccg accggaggcg gaatgcgggc gcccgtcggt
     9061 gacagcgg
//
LOCUS       DAORNH010000188         8922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5798_length_8922_cov_2.852261, whole genome shotgun sequence.
ACCESSION   DAORNH010000188 DAORNH010000000
VERSION     DAORNH010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5798_length_8922_cov_2.852261"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PK264_17010"
     CDS             <1..249
                     /locus_tag="PK264_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77608.1"
                     /translation="ITGDSNSNVLSSLGGADTMRGGGSLAGRFAVDAPNAAVGQFVYV
                     TVTRTLGWDLDGTGGAAIENIAVLQVGATLTINDLLLF"
     gene            complement(260..1069)
                     /locus_tag="PK264_17015"
     CDS             complement(260..1069)
                     /locus_tag="PK264_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRD77609.1"
                     /translation="MAHLGVPTTAFKAPMIYNPYFEAAGIDAVVVPMGCEAVDYPGFL
                     RLVVRLTNFAGALITMPHKVTTVALLDEATTTVKVAGSCNAVRRGAGGRLIGDMFDGE
                     GFVRGVARKGVRVGGASALVVGAGGVGAAIAASLAKAGVRRLALYDTSAASMGGLAGR
                     LRAHYPAIELVVGSKDPAGFDVVVNATPLGMSERDPLPVDVDQLSPATFVGEVVMKQE
                     ITPLLAAARARGCAYQVGTDMLFEQIPAYLEFFGLPTTTADELRRLARISY"
     assembly_gap    1071..1104
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1185..3059
                     /locus_tag="PK264_17020"
     CDS             1185..3059
                     /locus_tag="PK264_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRD77610.1"
                     /translation="MRPAIATVCLSGVLNEKLEAIAAARFKGVELFENDLLAYSGTAK
                     DVRRMAADLGLEIITYQPFRDFEGMPPERRDRVFQRAERKLDTMQELGCNLLMICSNV
                     SPESLGGIERAAADLHELGERAKKRGCRIGFEALAWGRHINDYRDAWEAVRRADHPAV
                     GLVLDTFHILVRKTDLGPVRAIPPDRIALVQVADAPLLDMDYLSWSRHYRNFPGQGDL
                     PLQAFMQALQSTGYDGPLSLEIFNDRFRAGSARSVAVDGHRSLIFMLDQTRAEAGPQK
                     NLPEPLPPRSRLLGTSFIEFAVDEDELPRFETLLSGLGFARSGEHRSKAVMRWTQGAI
                     NVVTNTDKDGFAHAFHLTHGTGVCAIGLAVEDAEATLARATALLDQPFTQAVGPGELE
                     IPAIRGLGGSMVYFLDRKGDLAKVWEIEFKPVAQTSLGADAGLIAVDHISQSMHYEEM
                     LTWLLFYNSLLDVMKPPEQDVLDPGGVVKSQVVQTADGALRLVLNASQSGRTQSSRFL
                     AEAFGSGVQHIAFATSDIFATVARLEQAGVMVLQIPENYYDDLEAKTDLSPEALDRLK
                     RHNILYERDGGGEYFQVYTETFGDLIFFEIVERRGGYKGFGATNAQVRLTAQSRGRKA
                     "
     gene            complement(3096..4603)
                     /locus_tag="PK264_17025"
                     /pseudo
     CDS             complement(3096..4603)
                     /locus_tag="PK264_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
     gene            complement(4648..5271)
                     /locus_tag="PK264_17030"
     CDS             complement(4648..5271)
                     /locus_tag="PK264_17030"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HRD77611.1"
                     /translation="MGRLILNSEVIGAGFWFILGGIVAWLGYDLGIGKLNEPGPGGAI
                     FWLGLGMVGLGAVVLVQTLASGAGQPVTTLWHGTRWPKIIAIVAALLILGYFFETVGF
                     IILVLGLLLLCMFFIDPVRWWVAIPVALIATVGIWGVMTEVLRIQLPGGILAEPIKLP
                     GGIVIPAIEDHLRAHFRVFLKFVNLGLGYITAVLAFVGGKLALLKFW"
     assembly_gap    5409..5418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5419..6289)
                     /locus_tag="PK264_17035"
     CDS             complement(<5419..6289)
                     /locus_tag="PK264_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD77612.1"
                     /translation="MRRRQFMFGACTAAAASSLLPGEALAEDPKDFPSKPITLIMPWS
                     AGTGIDLWHRAMGEVVSARLGQPVVVENKTGASGTAGPAVMAATGKPDGYVISHIPIT
                     IFRLPFTQKVTWDPIRDFSYIIHLSGFLFGVTVRTDSPFKTFKDLIDFARANPGKLTY
                     GSPGAGTSLHIGMEQIALKAGVKFTHVPFNQGSEAALLGGHIMCNTGGASWWPHTDAG
                     TMRVLVIWTKARNSRLPNVPTLEEEGFPFVFDSPFGIGGPRGMHPDIVKKLHDAFKVA
                     LADPKPREIMARYD"
     gene            complement(6324..7292)
                     /locus_tag="PK264_17040"
     CDS             complement(6324..7292)
                     /locus_tag="PK264_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD77613.1"
                     /translation="MKRRDFLASSAAAAGMLAAGPAFSQDKFPNRPITLIVPWPAGSG
                     VDLWHRALGEAAGRILGQPITVDNRTGGTGTSGPAAMAANAKPDGYTISQIPVTVFRF
                     PFIQKATYDPLKDFSYIIHLSGYTFGVVVRADSPFKTFQDLIAFARANPNKMTYATPG
                     AGSSLHLGMEQIALQAGVKFTHVPFKGGPEGWAALEGGHVMADADATGWAPLVDSGKF
                     RLLVLWTADRNPRWPTVPTLKETGFNFVIDSPFGFAGPKGMAPEVVKTLHDALKVAVD
                     DPKVLELMKKYDYPKRYMGTDDYVKFVTQQVEIEKGLVEKLGLAKK"
     gene            complement(7327..8316)
                     /locus_tag="PK264_17045"
     CDS             complement(7327..8316)
                     /locus_tag="PK264_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD77614.1"
                     /translation="MTDLIDRRSLIAGAGAAIATTGSLAAPALAQAKFPSRPVTLIIP
                     WPAGSATDLWHRAMAVPAEKALGQPIVVENKAGAAGTLGPATMAASAKPDGYTISHIP
                     ITVLRQPSIQKVTWNPLTDFSWIIGMSGFLFGTVVRADSPYKSLKDLIEYARANPRKV
                     TYGSPGAGTSLHLGMEQIARHAKVEWTQVPFKGSVESVAALEGGHITCLAGGTDWIPL
                     ALAGKLRVLAVWSEKRIPEFPEAQTLAEQGYPFVFDSPFGIAGPRGMDAAVVKTLHDA
                     FKVALEDETAKVVMKKYQFVPRYMATADYAKFVAELYEKEKAVVTALGLVKKD"
     gene            8538..8897
                     /locus_tag="PK264_17050"
     CDS             8538..8897
                     /locus_tag="PK264_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77615.1"
                     /translation="MTLSKQGWGLALVAAIAMVSTVEEAAALPPGQHHCGPSKQISRA
                     TSSDGKWCVTKFSTPVCRRDPRTGKDVLIGTETKTQKEEGACSASSRPTRPFKPVAPP
                     HLRPVRPPVFARRGARA"
BASE COUNT         1575 a         2888 c         2963 g         1452 t
ORIGIN      
        1 atcacgggtg actcgaactc gaacgtgctc tcgtctctcg gcggcgcgga cacgatgcgc
       61 ggcggcggct cgctcgcggg acgcttcgcg gtcgacgcac ccaatgccgc ggtcggccag
      121 ttcgtctacg tgaccgtgac ccgcacgctc ggctgggacc tggacggcac cggcggcgcc
      181 gcgatcgaga acatcgccgt cctccaggtc ggcgccacgc tgacgatcaa cgatctcctg
      241 ctgttctgag acgcaaaact cagtaggaga tccgcgcgag gcggcgcagt tcgtcggcgg
      301 tcgtcgtcgg caggccgaag aactcgaggt aggccgggat ctgctcgaac agcatgtcag
      361 tgccgacctg ataggcgcag cctcgcgccc gggccgcggc cagcagcggc gtgatctcct
      421 gcttcatcac gacctcgccg acgaaggtcg ccggcgacag ctgatcgaca tcaacgggca
      481 gcggatcacg ttcgctcatg ccgagtggcg tcgcattgac caccacatcg aaaccggccg
      541 gatccttcga gccgaccacg agctcgatcg ccggatagtg cgcgcgaagg cggccagcga
      601 ggccacccat gctcgcggca ctcgtatcgt agagggcaag ccggcgaacg ccggccttgg
      661 cgagcgaggc agcgatcgcg gcgccgacgc cgcccgcgcc gacgaccagg gccgaagcac
      721 ctccgaccct cacacccttg cgcgcgacgc cgcgcacgaa gccttcgccg tcgaacatgt
      781 cgccgatgag ccgaccgccg gcgccccgcc gcacggcgtt gcacgagccc gcgaccttca
      841 ccgtcgtcgt cgcctcgtcg agcagggcga ccgtcgtcac cttgtgcggc atggtgatga
      901 gtgcgccggc gaaattggtg agccgcacga cgaggcggag aaagcccgga tagtccactg
      961 cttcgcaacc catcggcacg acgacggcat cgatgcctgc ggcctcgaaa tacggattgt
     1021 agatcatcgg cgccttgaag gccgtcgtcg gcacgccgag atgggcgatc nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnntgttgc aaggccgcgt cctgccccat agtgctcgtt
     1141 ccagtggccg accataggct ctcgagccgt cgaaggatac gaccttgcgc cctgctatcg
     1201 cgaccgtctg cctgagcggc gtcctcaacg aaaaactcga agcgatcgcc gccgccaggt
     1261 tcaagggtgt cgagctcttc gagaacgatc tcctcgccta tagcggcacg gcaaaggacg
     1321 tgcgccgcat ggcggccgac ctcggcctcg agatcatcac ctatcagccc ttccgcgact
     1381 tcgagggcat gccgcccgag cggcgcgatc gcgtcttcca gcgcgccgag cgcaaactcg
     1441 acaccatgca ggagcttggc tgcaacctcc tcatgatctg ctcgaacgtg tcgcccgaga
     1501 gcctcggtgg catcgagcgg gcggcggccg acctccatga actcggcgag cgcgcgaaga
     1561 agcgcggctg tcgaatcggc ttcgaggcgc tcgcctgggg gcgccacatc aacgactatc
     1621 gggatgcctg ggaggcggtg cggcgcgccg atcatccggc cgtcggcctc gtgctcgata
     1681 ctttccacat cctcgtgcgc aagacggacc tgggtccggt ccgcgccatc ccgccggaca
     1741 ggattgcgct cgtgcaggtc gcggacgcgc cgctcctcga catggattat ctgagctgga
     1801 gccggcacta tcgcaatttc ccgggccagg gcgacctgcc gctgcaggcc ttcatgcagg
     1861 cgttgcaatc gaccggctac gacgggccac tctcgctcga gatcttcaat gaccgattcc
     1921 gggcgggctc ggcgcgaagc gtggccgtcg acggtcatcg ctcgctgatc ttcatgctcg
     1981 accagacgcg ggcggaggcc ggtccgcaga agaacctgcc cgagccgctg ccgccccgct
     2041 cgcgcctgct cggcacgtcc ttcatcgagt tcgcggtcga cgaggacgaa ctaccacgct
     2101 tcgagacgct gctctctggt ctcggctttg cgagatcggg cgagcatcgc tcgaaggcgg
     2161 tgatgcgctg gacgcaaggg gccatcaacg tcgtcacgaa caccgacaag gacggatttg
     2221 ctcacgcgtt ccatctgacc cacggcacgg gggtatgcgc gatcggcctg gcagtcgagg
     2281 atgctgaggc cacgctcgcc cgcgcgacgg cgctgctcga tcaacccttc acgcaggcag
     2341 tcggtccggg cgagctcgag attccggcga tccgcgggct cggcggcagc atggtctact
     2401 tcctcgatcg gaagggcgat ctcgcaaagg tctgggagat cgagttcaaa ccggtggcgc
     2461 agacaagcct gggcgcggac gcggggctga tcgcggtcga ccacatctcg cagtcgatgc
     2521 actatgagga gatgctgacc tggctcctct tctacaactc gctgctcgac gtgatgaagc
     2581 cgcccgagca ggatgtgctc gatccgggcg gcgtcgtgaa gagccaggtg gtgcagacgg
     2641 cggacggcgc gctccgcctc gtgctcaatg cctcgcagtc gggccggacg cagtcatcgc
     2701 gcttcctcgc ggaagcgttc ggctcgggcg tccagcacat tgccttcgca acgtccgaca
     2761 tcttcgcgac cgtcgcgcgg ctcgagcagg ccggcgtcat ggtgctgcag atccccgaga
     2821 actactacga cgacctcgaa gccaagaccg acctatcgcc tgaggcgctc gaccgattga
     2881 agaggcacaa catcctctac gagcgcgacg gcggcggcga gtatttccag gtctacaccg
     2941 agaccttcgg cgacctcatc ttcttcgaga tcgtcgagcg gcgcggcggc tacaagggct
     3001 tcggcgcgac caacgcgcaa gtgcgcctca ccgcgcagtc gcgcggcagg aaggcttgag
     3061 cgccgcgttg ctgccgcatg cgccgggccg ttgcgtcaat tcgtgtcgac cgccaccttg
     3121 tcacgagtct tctttatgcc aggcagcagc ggcacgatga gcacgatcgc ggcgagcacg
     3181 aggcagacga gcgagatctt gcgctcgagg aagatccaga agccgccggc cgattccgcc
     3241 gccgacatgg tgagcgactt cctgagctcg gtatcgagcc gcggaccgag cacgtaggcg
     3301 agcacgagcg gggccggctc gtagccgaat ttgcggaaga ggtagcccgc aacgccgaac
     3361 acggtcatga cgagcacgtc gaaggccttg ttctccgagg cgaacacgcc gacgacgcaa
     3421 aacatcagaa taagcgggaa gaggatgttg tagggcacct tcagcacctg cacccacatg
     3481 ccgatgagcg gcatgttgag gatgagcagc atgatgttgc cgatgtacat gctggcgaca
     3541 atgccccaga agatgtcctt ctggttctgc atcatcatcg gccccggctg cacgccgtgc
     3601 acgacgaagg cgccgagcag cagcgccatc accacgttcg gcgggatgcc gagcgtcatc
     3661 agagggatga aggcgcgttg ttggcagcct cggggcctgc gacgccctcg atggcgccct
     3721 tgccgaaatt ctccgggttc ttcgaagcct tgcgctcgat cgcgtaggat gcgaaggccg
     3781 cgatcacggc gccgccaccc ggcagcacgc cgaggaagaa gccgagcacc gtgccgcgca
     3841 cgattgccca tttcgaggct gcccagtcct tcaccgaggg ccagaggctg ccgatgcgcg
     3901 ccgtgataat ctcgcgcttc agcttggatt cgaggttcgc gaggatctct gcgacgccga
     3961 acaaccccat gacgatcggc acgaggccga cgccgtcgac gagctcgagc cggtcgaagg
     4021 tcatgcgcgc ctgcgctgtg atcggatcaa gcccgacgag gccgagcacg gtgccgaggc
     4081 aggccatcag cagcgccttg gcgagcgaac cttgcgtcat gaaggcgagc agcgtgaggc
     4141 cgagcaccat cagcgagaag tactcgggcg gaccgaaggc cacggcaaag cgcgcgagga
     4201 tcggcgcgac cagcatcagg agtacgatcg agaacgtgcc ggcgatgaac gaggccatcg
     4261 cggccatgcc gagcgcgggt ccggcgcggc cgcgctttgc catctcgtgg ccgtcgaggc
     4321 aggtgaccac ggatgccgcc tcgcccggga tgttgacgag gatcgacgtg gtcgagccgc
     4381 cgtacatcga gccgtagtag atccccgaca tcatgatgat gccggccgcc gggtccgact
     4441 tgagcgtgac cggaagcagc aacgacatcg ccgtcaccgg cccgatgccg gggagcacgc
     4501 cgacgagcgt gccgatgaac acgccgatga agcaggcgat cagattcttg ccgagcgtcg
     4561 ccacggtgaa gccgagcttc aggctggtgc cgacatcctg catggcggcg aagaaattcg
     4621 tcagaaggtc cacgggtcgc tagcctctca ccagaacttc agcagggcga gcttgccgcc
     4681 gacgaacgcg agcacggcgg tgatgtagcc gagcccgagg tttacaaact tgaggaagac
     4741 gcggaaatgg gcgcgcaggt gatcctcgat cgccgggatg acgatgccgc ccggcagctt
     4801 gatcggttcg gcgaggatgc cgccgggcag ctggatgcgc agaacttccg tcatcacgcc
     4861 ccagatgccg accgtggcga ttaacgcgac cggtatcgcc acccaccaac gtacggggtc
     4921 gatgaagaac atgcagagca gcaggaggcc gagcaccaga atgatgaagc cgaccgtctc
     4981 gaagaagtag ccgaggatca gaagcgcggc gacgatcgcg atgatcttcg gccagcgcgt
     5041 gccgtgccag agtgtggtca ccggctggcc ggcacccgag gcgagcgttt ggacgagcac
     5101 caccgcgccg aggccgacca tgccgaggcc gagccagaag atggccccgc ccggacccgg
     5161 ctcattgagc ttgccgattc cgagatcgta gccgagccag gcgacaatgc cgccgagaat
     5221 gaaccagaaa cccgctccga tcacctcgga gttcaagatc aagcgtccca ccgtcgtctc
     5281 ccccgagccg gccattttca gccgggctat ttcttggcgc cgagcccgag acgctcgacc
     5341 gttgccttct ggtccgcgac ctgctcggcc acgaacttcg tgtaagcggc gctgtccatg
     5401 tagcgctcnn nnnnnnnnag tcgtagcgcg ccatgatctc acgcggcttc ggatccgcca
     5461 acgcgacctt gaaggcatcg tgaagcttct tgacgatgtc ggggtgcatg ccccgcggcc
     5521 caccgatgcc gaaggggctg tcgaagacga acgggaatcc ctcttcctcg agcgttggga
     5581 cgttggggag ccgcgaattg cgcgccttcg tccagatcac gagcacgcgc atcgtgccgg
     5641 catcggtgtg cggccaccag ctggcgccgc ccgtattgca catgatgtgg ccgccgagga
     5701 gggcggcctc cgacccctgg ttgaacggaa catgcgtgaa cttcacaccc gccttcagcg
     5761 cgatctgctc catgccgatg tgcagcgagg tgccggcgcc gggcgagccg taggtgagct
     5821 tccccgggtt cgcacgggcg aagtcgatga ggtccttgaa ggttttgaag gggctgtcgg
     5881 tcctgacggt gacaccgaac aggaaccccg agagatggat gatgtagctg aagtcgcgga
     5941 tcggatccca ggtcaccttc tgcgtgaagg ggaggcggaa gatcgtgatc ggaatgtgcg
     6001 agatcacgta tccgtccggc ttccccgtcg cggccatcac ggcaggtcct gcggtgccag
     6061 aggcgcccgt cttgttctcg acgacgacgg gctgtccaag acgcgcggac acgacttcgc
     6121 ccatggcgcg atgccaaagg tcgatgccgg ttcccgccga ccacggcatg atgagtgtga
     6181 tcggcttcga gggaaaatcc ttcgggtctt ccgcgagagc ctcgccgggc agcagtgacg
     6241 atgctgccgc cgcggtgcag gccccgaaca tgaactgtcg acggcgcatc gtatctcctc
     6301 ccgagaccag taccgagtgg accctatttc ttggcgagcc cgagcttctc gacgaggccc
     6361 ttctcgatct ccacctgctg ggtgacgaac ttcacgtagt cgtcggtccc catgtagcgc
     6421 ttcggatagt cgtacttctt catgagctcg agcaccttcg gatcgtcgac ggcgaccttc
     6481 aaggcgtcgt gcagcgtctt cacgacctcc ggcgccatgc ccttcggccc ggcgaaaccg
     6541 aaggggctgt cgatcacgaa gttgaagccg gtctccttta gcgtcggcac cgtcggccag
     6601 cgtggattgc ggtccgcggt ccacaggacg aggaggcgga acttgcccga gtcgaccagc
     6661 ggcgcccacc cggtcgcgtc ggcatccgcc atgacgtggc cgccctcgag cgcagcccag
     6721 ccctcgggcc cacccttgaa gggcacgtgc gtgaacttga cgccggcctg cagcgcgatc
     6781 tgctccatgc cgaggtgcag cgagctgcca gcgccgggcg tcgcatacgt catcttgttg
     6841 ggattggcgc gtgcgaaggc gatcagatcc tgaaacgtct tgaacgggct gtcggcgcgc
     6901 acgacgacgc cgaacgtgta gcccgagaga tggatgatgt agctgaagtc cttgagcgga
     6961 tcgtaggtcg ccttctggat gaaggggaag cggaacacgg tgaccgggat ctgcgagatc
     7021 gtgtagccgt cgggtttggc gttggcggcc atcgcagccg gaccggacgt gccggtaccg
     7081 ccggttcggt tgtcgacggt gattggctgg ccgaggatgc gccccgcagc ctcgccgagg
     7141 gcgcgatgcc acaagtcgac gccgctgccc gcgggccagg gcacgatcag cgtgatcggc
     7201 cggttcggaa acttgtcctg cgaaaaggct gggcctgcgg cgagcatgcc ggcggcggcg
     7261 gccgaagagg cgagaaagtc gcggcgcttc atgggcgaaa gctcctcgaa tgtgacgtgg
     7321 cggtggttag tccttcttca cgaggccgag cgcggtgacg accgctttct ccttctcgta
     7381 gagttctgcg acgaacttcg cgtagtcggc cgtcgccatg tagcggggca cgaactggta
     7441 cttcttcatg acgaccttcg ccgtctcgtc ctcgagcgcg accttgaagg catcgtgcag
     7501 cgtcttcacg accgcggcat ccatccccct cggccctgcg atgccgaacg ggctgtcgaa
     7561 gacgaagggg tagccttgct cggcgagcgt ctgcgcctcc gggaactcgg ggatgcgctt
     7621 ctcgctccag acggccaaga cgcggagctt gccggcgagc gcgagcggga tccagtccgt
     7681 gccgccggcg aggcaggtga tgtgcccgcc ctcgagcgcc gcgacgctct cgacgctgcc
     7741 cttgaatggg acctgcgtcc actcgacctt ggcgtgtcgc gcgatctgct ccatgccgag
     7801 gtgaagcgag gtgccggcgc cgggcgagcc gtaggtcacc ttcctcggat tggcgcgcgc
     7861 gtattcaatg agatccttca gcgacttgta cgggctgtcg gcgcgcacga ccgtgccgaa
     7921 caggaagccc gacatgccga tgatccagct gaagtccgtg agcgggttcc aggtcacctt
     7981 ttggattgag ggctggcgga gcaccgtgat cggaatgtgc gagatggtgt agccgtcggg
     8041 cttcgcactc gccgccatgg tcgccgggcc gagcgtgcct gcggcgccag ccttgttctc
     8101 gacgacgatc ggctggccga gcgccttctc ggcgggaacg gccatcgcgc gatgccagag
     8161 gtcggtggca ctccccgccg gccacgggat gatgagcgtg acgggccggc tcgggaactt
     8221 cgcttgtgcc agggccgggg cagcgagcga tccagtagtc gcgattgcgg ctccggcgcc
     8281 agcgatgagc gaccggcggt cgatgagatc agtcatgtgt cctccctttg gacgttgcgg
     8341 ctctcatgtt ccggtcacca ctcatcgggc gcgacatcgg gggcgcgcgc cggttccgtg
     8401 gtcgaggcct ttgcggcctt cagttcccgg agcaaaagct gtggacgatg ttcaaatcac
     8461 tgtctgggcg cagcatctcc ctgtctgacg gctcgtgcga tggcgctgcg agcctggaga
     8521 gatggagaga tcaagtgatg accctttcga agcaaggctg gggcctggct cttgtcgcgg
     8581 cgatcgccat ggtttcgacc gtggaggagg cggcagcact gccgccgggc cagcatcact
     8641 gcggaccaag caagcagatc agccgtgcaa cgtccagtga cggcaagtgg tgcgtgacca
     8701 agttcagcac acccgtctgc aggcgcgacc cgaggaccgg aaaggatgtt cttatcggca
     8761 ccgagacgaa gacgcagaag gaggagggcg catgctccgc ttcgagccga ccgacccggc
     8821 cgttcaagcc ggtcgcgccg ccgcacctgc gcccggtgcg cccgccggtc tttgcgaggc
     8881 gaggggcgcg cgcctgacgc agcgacgccg aaacgatctc cg
//
LOCUS       DAORNH010000189         8921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5799_length_8921_cov_2.427363, whole genome shotgun sequence.
ACCESSION   DAORNH010000189 DAORNH010000000
VERSION     DAORNH010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_8921_cov_2.427363"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="gltA"
                     /locus_tag="PK264_17055"
     CDS             complement(<1..915)
                     /gene="gltA"
                     /locus_tag="PK264_17055"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HRD77616.1"
                     /translation="MSGQSGSAGGPARKAEADRTATVIVNNKQVQMPIRSGTIGPDVV
                     DVGRLYRDTGCFTYDPGFTSTANCSSKITYIDGEEGILLYRGYPIEQLAQKSNFIEVC
                     YLLVNGELPTKADLDKFQSTITRHTMVHEQMTRFYSGFRRDAHPMAVMCGVVGALSAF
                     YHDSTNIDDPVHRMIASHRMIAKMPTIAAMAYKYSIGQPFIYPRNDLDYASNFLQMCF
                     AVPCEPYVVNPVLSRAIDRIMILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLW
                     GPSHGGANEAALKMLEEIGSVDKIPEFVK"
     gene            complement(1088..2110)
                     /locus_tag="PK264_17060"
     CDS             complement(1088..2110)
                     /locus_tag="PK264_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD77617.1"
                     /translation="MGSVPLAEREVTVVGAGIVGLWQALTLLKRGHRVTLIEAGDATF
                     AQSASRLAGAMLAPFCEAEASEPIVTRLGLEALSLWKAVYPGITWRGSLVVAAARDQG
                     ELTRFHRMTEGHRWLDASEVGALEPELAGRFGRALFYPDEGHVAPRAAMGHLLAELRA
                     GGADVRLGIRADVAALTNDGGIVIDCRGRAAIGEVPGLRGVRGEMALVKAPDLAVSRP
                     IRLLHPRLSPYIVPWGDGHYMIGATVIERSDAGLVTVRSALDLLGAAFALHPGFAEAE
                     IVELATGVRPAFVDNIPKAVIGHNGSIVVNGAYRHGFLLSPVLAARVASYIETGRIDN
                     AVMSAA"
     gene            complement(3253..4761)
                     /locus_tag="PK264_17065"
     CDS             complement(3253..4761)
                     /locus_tag="PK264_17065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRD77618.1"
                     /translation="MTGPRNGERAAGSLGLSLGLALALILVLRIVALWFNRTDLFFDE
                     AQYWSWGLEPAFGYYSKPPLVAWIIRVSTELCGTSEFCVRLPSPLIHTATAIVLYWLG
                     RRLAGPAAGFWASLTFATLPGVSFSASLMSTDVPLLLAWAVALLALSILLTGRSWWPA
                     IVLGLAIGVGLNAKYAMAFFGGCLAVYLVATPEQRGFMKDGRLWLAISIAAALITPNM
                     LWNQANSFATFSHTADNANWAGRLPSPLRLVEFLGAQAGVFGPILVGALIALIVRAWR
                     EGLPAVERLLLAFTLPVIALISVQAFLSRAHANWAATAYVAGSVLVPATLVRLGREGW
                     LKASLWLHLAIAAIIGITLAFAGEFKLPGIPDPFARTLGWKATAETTAHNLVRGAAEG
                     KPFAAVVTDERAATAELLYYRRDISYLVYAWRAGPRPLDHYELTRPWRPGTEPLLLVS
                     LRSEAEPILSRFEKVELVEEAAIPTGRHATRRVRFYRLSGYRPSAVPTQPKR"
     gene            5021..6154
                     /locus_tag="PK264_17070"
     CDS             5021..6154
                     /locus_tag="PK264_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD77619.1"
                     /translation="MIPESEFNAEALLAGILPWVEVESPTWHVAGVNRMMDLAAAEVQ
                     RLGGTIERVPGVDGAGDIVIGRFPGADPTAGKGILVLSHLDTVHLVGTLADKLPIRRQ
                     GNRCYGPGIYDMKGGVRIALHAFDVVRRLDRSPRLPVTFMFVPDEEVGSPTSRARIEA
                     EAKAHKYVLVTEPCKNGVIVTGRFAFQRFWVETKGRPAHAGANNRDGRSAIRAMASLV
                     ERIEGMTDFTRGMTFSVGTIEGGTFVNVMPISCRAQVLTVAPNEPIFHEVRERMMALA
                     IEHDGVTISVTQGPVRPLWSPHMATMALYERARLMSAELGFDLRHGSFGGGSDGNFTG
                     ALGVATLDGLGVDGAGAHTFEEHLLVSSLPRSRLLAELLARLD"
     gene            6194..6889
                     /locus_tag="PK264_17075"
     CDS             6194..6889
                     /locus_tag="PK264_17075"
                     /inference="COORDINATES: protein motif:HMM:NF016532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HRD77620.1"
                     /translation="MLQTLTTNLDLTAFAWFVVTISLYRLTLRSASLERRSIVGAVQE
                     HRIAWMKNMALRDPRVLDAVLLGSLSHGNAFFASTAAIGIGGLAALFGTGDKAQAIIE
                     RLPYAQKSTAMLYEVKLILLMAIFVYAFFKFVWAFRLSHYVAIMIGSTPLPKADNTDE
                     CELHARRTARLIGIAAEHANGGLRSFYYAFAALTWFYHPLLFMACTTGVLAIMIRRDY
                     FSRSRRLLDGTWP"
     gene            6977..7834
                     /gene="sseA"
                     /locus_tag="PK264_17080"
     CDS             6977..7834
                     /gene="sseA"
                     /locus_tag="PK264_17080"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HRD77621.1"
                     /translation="MPSNPRRWLVETGWLADHLDSPDVVVLDGSMHLPTSGRNAREEY
                     KAAHIPGALYFDIDEVSDKKNPLPHMLPSTVQFASQMKKMGIGDGLQIVVYDSEGLYS
                     AARVWWMFRTMGHDNVAVLNGGLKKWKAEGRAISGEPPAPRTPRHFTPRFNGALVKDW
                     EDVKGLIGHKSTQILDARAAARFAGTAPEPRKGLRSGHIPSSKSLPFTSLLNTDGTLK
                     SVAELRAVFEKAGVDIDRPVVASCGSGVTAGVIAFALGQLGRPDAAVYDGSWSEWGAR
                     EDLPIETGA"
     gene            complement(7854..>8603)
                     /locus_tag="PK264_17085"
     CDS             complement(7854..>8603)
                     /locus_tag="PK264_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD77622.1"
                     /translation="GGINAMIADSSALPEQVVRDVVRSAYDSAGQRCSAARLLFLQEE
                     VADKTIEMLVGAVEALEIGDPIEMNTDIGPVIDQGAQDALEAHKARMQRQGQQLVDRR
                     LPEACRVGTYVTPAAYEIASPAFLEREVFGPILHVVRFSGGHLLRVVESLNAMGFGLT
                     LGVHSRIGAVADLVAEHARVGNLYVNRNQIGAVVGVQPFGGEGLSGTGPKAGGPNYVA
                     RFATERTRTTDITATGGNVQLLGLGVVDKTG"
     assembly_gap    8604..8684
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8685..>8921)
                     /locus_tag="PK264_17090"
     CDS             complement(<8685..>8921)
                     /locus_tag="PK264_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRD77623.1"
                     /translation="PWNFPIAIFTGQVAAALAAGNPVLAKPAGQTPVTAFLATELLHQ
                     AGVPKGVLHLLPGGGPVGAQLVKDRRVKGIAFTG"
BASE COUNT         1517 a         2968 c         2981 g         1374 t
ORIGIN      
        1 cttcacgaac tcggggatct tgtcgacgct gccgatctct tcgagcatct tgagcgcggc
       61 ctcgttggcg ccgccgtgcg agggacccca gaggcacgcg attccggccg cgatgcaggc
      121 gaacgggttg gcgcccgagg agcccgcgag acgcaccgtc gacgtcgagg cgttctgctc
      181 gtgatcggcg tgcaggatca tgatgcggtc gattgcgcgt gagagcacgg ggttgacgac
      241 atagggctcg cagggcaccg cgaagcacat ctgcaggaag ttcgacgcgt agtcgaggtc
      301 gttccttgga tagatgaacg gctgaccgat cgagtacttg taggccatcg ccgcgatcgt
      361 cggcatcttg gcgatcatgc ggtgcgaggc gatcatgcga tgcacggggt catcgatgtt
      421 cgtgctgtcg tgatagaagg ccgacagcgc accgacgacg ccgcacatca ccgccatcgg
      481 atgcgcgtcg cgccggaagc ccgaatagaa gcgcgtcatc tgctcgtgca ccatcgtatg
      541 gcgcgtgatg gtgctctgga acttgtcgag atcggccttg gtcggcagct cgccgttgac
      601 gagcaggtag cagacctcga tgaaattcga cttctgggcg agctgctcga tcgggtagcc
      661 gcggtagagg aggatgcctt cctcgccgtc gatataggtg atcttggacg agcagttggc
      721 cgtcgaggtg aagcccggat cgtaggtgaa gcagccggta tcgcggtaga gccggccgac
      781 gtcgaccacg tcgggaccga tggtgcccga ccggatcggc atctgcacct gcttgttgtt
      841 gacgatcacg gtcgctgtgc gatccgcctc ggctttacgt gccggtccgc ccgccgatcc
      901 actctgtcca ctcatgggtc ccgtccttcg ctcggccggc aagctgtcgc tggccagcct
      961 ctacgccacc atctcgcatg tgcgtaaacc agaatagcgc cagcggcaac cggatggcga
     1021 gccaaacgaa agaagggtaa gcgcggcagg ttggcgcgag gatagcgagg cctctccagc
     1081 ccgccgatca ggcggcagac ataacggcgt tgtcgatgcg gccggtctcg atatagctcg
     1141 cgacccgtgc agccaggacc ggtgacagca ggaagccatg gcgataggcg ccgttgacga
     1201 cgatcgaacc gttgtgcccg atcacggcct tggggatgtt gtcgacgaag gccggccgga
     1261 ccccggtcgc aagctcgacg atctcggcct cggcgaagcc tgggtgcagc gcaaacgcgg
     1321 cgccgagcag gtcaagcgcg gaacgcaccg taacgaggcc cgcgtcgctg cgctcgatga
     1381 cggtcgcgcc aatcatgtag tgcccatcgc cccacggcac gatgtagggt gagaggcgcg
     1441 gatggagcag gcgaatcggc cgcgagaccg caaggtccgg cgccttgacg agcgccatct
     1501 cgccgcgcac acccctgagc cctggaactt cgccgatcgc cgccctgccc cggcaatcga
     1561 tcacgatgcc gccgtcattg gtcagcgcag cgacatcggc acggatccca aggcgcacat
     1621 cggcgccgcc cgcgcgtaac tcggcaagca gatggcccat tgccgcgcgc ggcgcgacgt
     1681 gtccttcgtc cggatagaag agggcacgac cgaagcgtcc tgccagctcg ggctcgaggg
     1741 cgccgacctc ggacgcgtcg agccagcgat gcccctcggt catgcggtgg aagcgggtga
     1801 gctcgccctg gtcgcgtgcg gccgcgacca ccagggagcc gcgccaggtg atgccaggat
     1861 agactgcctt ccagagcgac agggcctcga ggccgagccg cgtcacgatc ggctcggagg
     1921 cctccgcctc gcagaacggc gcaagcatgg cgcctgcaag ccggctcgcg gattgggcaa
     1981 acgttgcgtc gcccgcctcg atgagcgtga cgcggtggcc gcgcttcaag agcgtgaggg
     2041 cctgccaaag ccccacgatg ccggcgccaa cgacggtgac ttcacgctct gccaatggca
     2101 cgctccccat ttcgcaggcg caccatagcc gacaggagat gtcccgtcct gaggtcgatc
     2161 aaggcgagag gcggtcctga tccacgaatt tgggaagcct ggattggggc gcggcgggct
     2221 cggacgccga cgacggcgtg cttcgccgcg ggtgagactc ggacgcggcc cggggcgcgg
     2281 gcttcgcttc gaccggctgg gccttgacca gggccttcgg tgctggacct ggcggcggat
     2341 cggtggccat cggcggcatc atcgatgcgc cgagcctcat gggaggcggc gccacggtcg
     2401 tgggcgccgg cgatacgggt tcgatggtcg gacctgggtc gagggccggg gccggcctcg
     2461 ccaccgcgcc agcgggggcc gggacggatg gcgcaacgat gatcggcgac tggtcggcag
     2521 ccggagcagc aaccggcgac gcggtcacgg gagccgtcgc tggagcagtc tgaggctcgg
     2581 gcggctgctt gaccgccgca ggagcgggca tcgcaatggc ggccggctcg acaatcggcg
     2641 tcggcgcaac gggggcgatg atgctcgagc cggtgggcgc catcggagcg aatcgccccg
     2701 gttcgagcca gctgaagcgc gccggtggat caatcgtcgg aaagctcgtc cgtacgatca
     2761 gaccgggtgt gatggcggga ggtgacgtct caggtgccgt ttcgccaacc ggtgcggcga
     2821 cctccggcgc gcgatggcgc cgcagcgcgc cccacgcggg tttttccgcc acctgacgcc
     2881 gctccgcggc aaatgcgagg agacgcgcgg cgctctcctc ggcaacctga cgattgtgca
     2941 cgagcgcggc gaacccgatt gccgagccgg cgatcaggcc gacagtcaga atgccgagca
     3001 cgcccgaccc tcgcgggggc cgccgggcgt gctgatcgat gctgggagac gacgagcgca
     3061 tgacgaaccc gatgacgtgg gccgcggagg cgcgcctcgg cggcgacagg aacgctgaac
     3121 gcaaccggca tgacggggga aactcaatca gtcgcgaaac ggagcaacga ctgtagtcgc
     3181 catcagccga cgcgcactag acaattgata aatcgacgat tttacttaag gcgacgttaa
     3241 tggcggagcg cgctatcgct tgggctgcgt cggcacggcg gacggcctgt agccgctcag
     3301 cctgtagaag cgcacgcgcc gcgttgcatg gcggcccgtc ggtattgccg cctcctcgac
     3361 gagttcgacc ttctcgaacc tgctgagaat cggttcggct tcggacctga ggcttacgag
     3421 cagcagcggc tccgtcccgg gcctccacgg ccgtgtcagc tcgtagtgat cgagcggccg
     3481 cggcccggcc cgccatgcgt agacgaggta tgagatgtcc cgtcgataat agagaagctc
     3541 agcggtagct gcacgctcgt cggtaaccac ggcggcgaat ggcttgccct ctgcggcacc
     3601 cctgacgagg ttatgcgcgg tggtctcggc ggtggccttc cagccgaggg tgcgcgcgaa
     3661 cggatcgggg atgccgggca gcttgaactc gccggcaaat gcgagtgtga tcccgatgat
     3721 cgcagcgatc gcgagatgca gccagagcga cgccttgagc cagccctccc tgccgaggcg
     3781 gacgagggtc gcaggtacca gcaccgagcc cgccacgtaa gcggtcgccg cccaattggc
     3841 gtgcgctcgc gacaggaacg cctgaacgga gatcaacgca atcaccggca gcgtgaacgc
     3901 gagcagcagc cgctccaccg ccggcagccc ctcgcgccac gccctgacga tgagggcgat
     3961 gagcgcgccg accaggattg gcccgaacac gccggcttgg gcgcccagga actccaccaa
     4021 tcgcagtgga ctaggcagcc ggcccgccca gttcgcattg tcggcggtgt gcgagaaggt
     4081 cgcaaagctg ttcgcctggt tccaaagcat gttcggcgtg atcagcgccg cggcgatcga
     4141 tatggcgagc cagagccggc cgtccttcat aaagccgcgc tgctccgggg tcgccacgag
     4201 atagacggcg aggcagccgc cgaagaacgc catcgcatac ttggcgttga ggccgacgcc
     4261 gatcgcgagt ccaaggacga tcgccggcca ccaggaacgg cccgtcaaca gtatgctcag
     4321 cgccaggagc gccaccgccc aggcaagtag caacggcacg tccgtcgaca tgaggctcgc
     4381 cgagaacgac acgccgggca gcgtcgcgaa ggtgaggctc gcccagaagc cagcagcagg
     4441 ccccgcgagc cgccgcccga gccaatagag cacgatggct gtcgccgtgt ggatgagggg
     4501 cgaggggagc cgcacgcaga attcgctcgt cccgcagagt tcggtcgaca ctcgaatgat
     4561 ccaggcgacc agcggcggct tcgaatagta gccgaaggcg ggctcgagcc cccacgacca
     4621 gtactgcgcc tcgtcgaaga agaggtcggt ccggttgaac cagagcgcca cgatgcgcaa
     4681 caccagaatg agggcgagcg ccagcccgag ggagaggccc aggctgcccg ctgcccgctc
     4741 gccgttccga ggcccagtca tgattgcatt gcggcccacg agcccatccg atcccgaacc
     4801 actcgatctc attgccattg cctcgcgcgg gtagcccgaa ttcatgcccc gaccaagtca
     4861 atcacgcgtc attcctcact tatttcgtgg gcttgtgccc ctgccacaaa ggcgcattgc
     4921 atctaccagc ttccggagcg acaattgcgc gaggcggtcg agaggtccgc ggcactgatg
     4981 gtgccaagcc gagacccggg cgggaccgta gggagcggag atgatcccgg agagcgagtt
     5041 caatgccgag gccctgctgg caggcatcct gccctgggtc gaggtcgaaa gcccgacgtg
     5101 gcatgtcgca ggcgtcaacc ggatgatgga tctggcggca gccgaggtgc agcgtctcgg
     5161 cggcaccatc gagcgtgttc cgggcgtcga tggcgcaggc gacatcgtca tcggccggtt
     5221 tcccggcgcc gatccgactg cgggaaaagg catcctggtc ctctcgcatc tcgacacggt
     5281 gcacctcgtc ggaacgctcg cagacaagct gccgatccgc cgccagggca accgctgcta
     5341 tggccccggc atctacgaca tgaagggtgg tgtcaggatt gcactgcacg ccttcgacgt
     5401 ggtgcgccgc cttgaccgca gtccccggct cccggtgaca ttcatgttcg tccccgacga
     5461 ggaggtcggc agcccgacat cgcgggcgcg catcgaggcc gaggccaagg cgcacaagta
     5521 cgtgctcgtc accgagccct gcaagaacgg cgtgatcgtc accggccgct tcgccttcca
     5581 gcgcttctgg gttgagacca agggccgccc cgcacatgcc ggcgccaaca accgcgacgg
     5641 ccgcagcgcc atccgcgcca tggcgagcct cgtcgagcgc atcgagggca tgaccgactt
     5701 cacgcgcggc atgaccttct ccgtcggcac gatcgagggc ggcaccttcg tcaacgtgat
     5761 gccgatctcg tgccgcgcac aggtactgac ggtcgcgccg aacgagccga tcttccacga
     5821 ggtgcgagag cgcatgatgg cccttgcgat cgagcacgac ggtgtcacga tctcggtgac
     5881 gcagggccct gtgcgtccgc tctggtcgcc ccacatggcg acgatggcgc tttatgagcg
     5941 cgctcggctg atgtcggccg agctcggctt cgacctccgt cacggctctt tcggcggtgg
     6001 cagtgacggc aatttcacag gcgcgctagg cgtcgccacg ctcgacggcc tcggtgtcga
     6061 cggcgctggc gcccacacct tcgaggagca cctgctcgtc tcgtcgctgc cacgctctcg
     6121 cctcttagca gagcttcttg cgcgcctcga ttgattgccc ggcaagcacc cgacaacgac
     6181 aagaaggctc cgcgtgctcc aaacgctcac aaccaatctc gacctcaccg ccttcgcctg
     6241 gttcgtcgtc accatctcgc tctatcgatt gacgctccgc agcgcctcgc tcgaacgccg
     6301 ctcgatcgtc ggcgccgtgc aggagcaccg catcgcctgg atgaagaaca tggcgctgcg
     6361 cgatccgcgc gtgctcgacg cggtgctgct cggcagcttg agtcatggca acgctttctt
     6421 cgcttcgacg gcggcgatcg gcatcggcgg tctcgcagcg ctcttcggca cgggcgacaa
     6481 ggcgcaggcg atcatcgagc gcctccccta tgcgcagaag agcacggcga tgctctacga
     6541 ggtgaagctc atcttgctga tggcgatctt tgtatatgcc tttttcaaat tcgtctgggc
     6601 gttccgcctc tcgcactacg tcgccatcat gatcggctcg acacctctgc cgaaggccga
     6661 caacacggac gaatgcgagc tgcatgcgcg ccgcaccgcg cgcctcatcg gcatcgcggc
     6721 cgagcatgcc aatggcgggc tccggtcgtt ctactacgcc ttcgcggcgc tgacctggtt
     6781 ctatcacccg ctgctcttca tggcctgcac cacaggcgtg ctcgcgatca tgatccgccg
     6841 cgactacttc tcgcgctcgc gccgtcttct cgatggcacc tggccgtgat gcagcgcgca
     6901 ccccacagct agacatcgtg agcggccgcg ctatccttcc ggtcaaagcg aagagaccaa
     6961 cccgaggata tcgcccatgc catccaaccc ccgccgctgg ctcgtcgaaa ccggctggct
     7021 cgccgaccat ctcgacagcc ccgacgtcgt cgtcctcgac ggctcgatgc acctgccgac
     7081 ctcgggccgc aatgcgcgcg aggagtacaa ggccgcgcac ataccgggcg cactctactt
     7141 cgacatcgac gaggtctcgg acaagaagaa cccgctcccc cacatgctgc cctcgaccgt
     7201 tcagtttgcc tcgcagatga agaagatggg gatcggcgac ggcctgcaga tcgtcgtcta
     7261 cgacagcgag gggctctact cggcggcgcg cgtgtggtgg atgttccgca ccatgggcca
     7321 cgacaatgtc gccgtgttga acggcggcct caagaaatgg aaggccgagg gccgcgcgat
     7381 cagcggcgag ccgccagcgc cgcgcacgcc gcgccacttc actccgcgct tcaacggcgc
     7441 gctggtcaag gactgggagg acgtgaaggg cctcatcggc cacaagtcga cgcagatcct
     7501 cgacgcccgc gccgcggccc gcttcgccgg caccgcaccc gagccgcgca aagggctccg
     7561 atcgggccac attccctcat cgaagagcct gccgttcacg agcctcctca atactgacgg
     7621 caccttgaag agcgtcgccg agctccgcgc cgtgttcgag aaggcgggcg tcgacatcga
     7681 ccgtcccgtg gtcgcgagct gcggctcggg cgtgactgcg ggcgttatcg ccttcgccct
     7741 cggccagctc ggccgccccg acgccgccgt ctacgacggc tcctggtccg aatggggcgc
     7801 ccgcgaggac ctgccgatcg agacgggcgc gtgagcggca gcgatccgca accctatccc
     7861 gtcttgtcga cgacgccgag gcccaggagc tgcacgttgc ctccggtcgc ggtgatgtcg
     7921 gtcgtgcgcg tgcgctcggt tgcgaagcgg gcgacgtagt tggggccgcc ggccttcggg
     7981 ccggtgccgg agaggccttc gccgccgaac ggctgcacgc cgacgacggc gccgatctgg
     8041 ttgcggttga cgtagaggtt gccgacgcga gcatgctcgg cgacgagatc ggcgacggcg
     8101 ccgatgcgcg agtgcacccc gagggtcaat ccgaagccca tcgcgttgag actctcgacg
     8161 acgcgcagga gatgccctcc cgagaagcgc acgacgtgca ggatcggccc gaacacctcg
     8221 cgctcgagga aggccggcga ggcgatctcg taggcggccg gcgtcacgta ggtgccgacg
     8281 cgacaggcct cggggaggcg gcggtcgacg agctgctggc cctggcgctg catccgtgcc
     8341 ttgtgcgcct cgagggcgtc ctgtgcgcct tggtcgatga ccggcccgat gtccgtgttc
     8401 atctcgatcg gatcgccaat ctcgagcgcc tcgacggcgc cgaccagcat ctcgatcgtc
     8461 ttgtcggcga cctcctcctg gaggaacagg agacgcgccg ccgagcagcg ctggccagcc
     8521 gaatcgtaag ccgagcgcac gacgtcgcgc accacctgct cggggagcgc gctgctgtcg
     8581 gcgatcatgg cgttgatgcc gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacccgg tgaaggcgat
     8701 gcccttgacg cggcggtcct tgacgagctg cgcgccgacc ggaccgccgc ctgggaggag
     8761 atgcaggacg cctttcggca cgcccgcctg atgcaagagc tcggtcgcga ggaaggccgt
     8821 gaccggcgtc tgccccgcgg gcttggcgag caccggattg ccggccgcca gcgccgccgc
     8881 gacctggcct gtgaagatcg cgatcgggaa gttccagggc g
//
LOCUS       DAORNH010000190         8890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5826_length_8890_cov_2.623995, whole genome shotgun sequence.
ACCESSION   DAORNH010000190 DAORNH010000000
VERSION     DAORNH010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5826_length_8890_cov_2.623995"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PK264_17095"
     CDS             complement(<1..1138)
                     /locus_tag="PK264_17095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRD77624.1"
                     /translation="MRVFVGICTLAALLAVTASTSGEARSGPRAPSTTASAPLTFAAG
                     CATGDRATLAAVGDLLFHDAVQARALAPGRSFRALWAPLLPVLEGADLSYGNLEGPAA
                     RGVRIGGIAVDDPGRRLDGIVYGRSRGALVFNYHPSVIGDLAASGFKVLSTANNHAAD
                     RGPLGIDRTIDELAAHELAYSGTRRRGDALSPWGTIVAAAGMKIGWLACTYSTNGMPD
                     KLGQVLNCYANKAAILDEVRRMRAGGAADAVIVAPHWGHEGSHVPLKADRAWAREMIE
                     AGAAAIVGTHPHVLQPWERVTASDGREGLVIYSTGNFISNQRSLTQRTGIVALVELVR
                     GAGGAVVSAAGFIPTWVDIEGGHVVREMPVDRADALRSALRLLAA"
     gene            1296..2129
                     /locus_tag="PK264_17100"
     CDS             1296..2129
                     /locus_tag="PK264_17100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD77625.1"
                     /translation="MVFLAVVLAAIMHAGWNAVIKGGGDPFATLTHMSMMNAVISLAV
                     IPFVPWPRPETWAWLVASVVIHSAYRLLLINAYRLGDLTHVYPIARGAAPLVTAIATF
                     ALIAETIAPTGYIAIALLSLGVVLMSLKGGRLGNLDGKVTGFALLSAASTSAYTLVDG
                     LGARANGSGIGFAVWMFFLNALAMQVIAGFWRGPAVWTSVAGAWRPALAGSALSMGAY
                     AIVIWAMTLAPIALVASLRETSVLFAALIGIIVLGEPVTRWRILAALAIGAGMVLIKA
                     S"
     gene            2126..3346
                     /locus_tag="PK264_17105"
     CDS             2126..3346
                     /locus_tag="PK264_17105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD77626.1"
                     /translation="MSDPAPSAAPADVPWRTIWLLALVCFASSANLRIADALLAQVAE
                     DFRVTVGQASSIVAAFAVSYGLLQAVYGPVGDRLGKLRVVAFGSALAAVFTGACAIMG
                     SLAALTAGRFLAGAAAAAIIPLSMAWVGDRVPFERRQPVLARFLTGQMSGLIFGQAAG
                     GIVGDLLGWRGAFAMLALVHAGATVVLVQHLHATPGLGAASSAPASLRQTLASIRSML
                     TRSYPRLVLAAVALEAAAMYGAFAYVGADLKARFGIGYGLIGLILGTFGLGGLIYAFL
                     APRLLAVIGERGFAISGGLVLAASYLALAFAGSLAIITVAVLAMGFGFYLLHNTLQVN
                     ATQMAPEARGLSVAVFAAVLFMSQSVGVAMAAPFIDSHGARPIYFVAALLLPLTGLLL
                     GLRLAKERALSIRP"
     gene            complement(3336..4058)
                     /locus_tag="PK264_17110"
     CDS             complement(3336..4058)
                     /locus_tag="PK264_17110"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HRD77627.1"
                     /translation="MKSLTSKSSILVLTGAGISKESGLDTFRDADGLWAKVRIEDVAT
                     PEAFERNPSLVQDFYNKRRQGMAGVRPNAAHLALARLEKAWPGAFRIVTQNIDNLHEA
                     AGSRRVVHMHGELLKALCRNCRGRHPWMGDLGADVSCPTCRKAGGMRPDVVWFGEQPY
                     HMEEISWAVMRADLFISIGTSGNVYPAAGFVATARSAGAYTVELNLEPSDGASSFHEC
                     RHGRATEIVPTFVDEVLAALKA"
     gene            complement(4071..4475)
                     /locus_tag="PK264_17115"
     CDS             complement(4071..4475)
                     /locus_tag="PK264_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRD77628.1"
                     /translation="MNVVDTSAIIAILEGEPERDAFARRTSEASYAIMSAVSMLEASI
                     VMSGRRGRAGVQALDEWFVGARIIIRPFDRSMAESAVAAYERFGKSSGSETRLNFGDC
                     CSHAFASLERAPLLFKGADFAATDIVPATTIP"
     gene            complement(4472..4735)
                     /locus_tag="PK264_17120"
     CDS             complement(4472..4735)
                     /locus_tag="PK264_17120"
                     /inference="COORDINATES: protein motif:HMM:NF019324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HRD77629.1"
                     /translation="MTLQLSAEIEGLVRRIAKRTGRAPDDVVREAIEAAARAAGVDKR
                     LAVGPGEVMELARENSGRVAALPLLYPRTPDEIIGYDESGLPR"
     gene            complement(4786..5817)
                     /locus_tag="PK264_17125"
     CDS             complement(4786..5817)
                     /locus_tag="PK264_17125"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRD77630.1"
                     /translation="MTKPAAGPSSNKKRVLYISHGTEDLYQMVRAAVPQGLELITLKS
                     NDEAERLEALSRADAVIVAGAKLTRERIAAAKNLKFVHHQGVGYHDTVDCAALAERKV
                     PLAITPGGTEQAVAEHTILLMLATMKRLPYMDAEMRAGRFHNYSMRHNTYEIGGKTIG
                     IVGLGRIGKGLARRLKPFGVRILYHDLLTFPPELERELEVTRVPFDKLIADSDIVTIH
                     VPRTPVTQGMFNAKVFERMKPTAFLINAARGGIVNEPDLVAALKAGKIAGAGLDVFDV
                     EPAPKNDPLYQFPNVVLTPHVAAGSRDAFTAKMRFVFKNLEAFFDGKPIEHGVDYVAE
                     MRAAGLKIG"
     gene            complement(6006..>7020)
                     /locus_tag="PK264_17130"
     CDS             complement(6006..>7020)
                     /locus_tag="PK264_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77631.1"
                     /translation="VAPRAPASTGWFLRRAAIGIAVLALAIAGGAWLMHSAIDPEAEG
                     PAVEARNDALAGVASWAFRDVGQTAEVGAGDDLVVVPATRDRQLLSRADVQALRHRTG
                     GGDRVVLAHVSLAEIADSSDMWRSDWVVDVSPGDSSRSGVTFPRVPAAGAPAWLAREN
                     AVRRTTFPVRFWQSDWQARLIGRSFSLINRLVAIGVDGIYLTGGDAAQSWSLERPGAR
                     DDLAALVRRIASHVRQLKPELLIVLENGDELVDDRRIRLAVDAFAITELFYSGSGAGE
                     PIADEAIDARLARLRPLQIDARPILVREQLLDARSVAAVSARVRRLGLVPAVEARSTG
                     VRP"
     assembly_gap    7021..7048
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7301..>7558)
                     /locus_tag="PK264_17135"
     CDS             complement(7301..>7558)
                     /locus_tag="PK264_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77632.1"
                     /translation="AQVPLLTLIAVHAPAAARATWFALTASLMSLAIVASQLLTKYLN
                     LLFPIERGQYDNLSALVGGVLGSSLGLPLLAVAFFWRTARK"
     assembly_gap    7559..7596
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7597..8820)
                     /locus_tag="PK264_17140"
     CDS             complement(<7597..8820)
                     /locus_tag="PK264_17140"
                     /inference="COORDINATES: protein motif:HMM:NF015078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77633.1"
                     /translation="MSATGNIPTDRRGVIERAADSVLAPARAFRLAYLPLLMVYFASG
                     ALGIIAVADSFWVKKSLTLTPADLASLAVWLQLPWSIKMVVSEVVDCVAIMGSRRRVY
                     ILLGAGLIASGLLLLAAAASGAVTAIPPERAYVIAQLLVVAGGIIQEVVADALSAEVV
                     ARVRPDGAPRSPADVDSDLAMVQVLSRLTYSIGAFLVGFLAGRLAESLPYHVVFLIGL
                     AVPAISILGALVVSLPNVLAAAHARRIDWRILGGGLAFAVIAVALGLSEMPFGQEALF
                     LIALGVILAMARLAMAELDPAIARRIAAAAAMIFAFRAAPTLGDGYRWFLIDRHGFDE
                     RFFGVLQVTGTGIGLGAMWLLTDTVMRRTVTVVLLALTIVAAILWLPSLLLVSGGHRW
                     TYDMFGLGPRGIALFD"
BASE COUNT         1444 a         3044 c         2892 g         1444 t
ORIGIN      
        1 ccgcggccag caggcgaagt gccgacctca gcgcgtccgc gcgatcgaca ggcatctcgc
       61 gcaccacatg cccgccctcg atgtcgaccc aggtcgggat aaagcccgcg gctgatacga
      121 cggcgcctcc agcgccccgc acgagttcca cgagcgcaac gatgccggtg cgctgcgtga
      181 gactgcgctg gttggagatg aagttgcccg tcgagtagat gacgaggccc tccctgccat
      241 cgctcgccgt gaccctctcc catggttgga gcacgtgcgg gtgcgtaccg acgatagccg
      301 cggctcccgc ctcgatcatc tcgcgagccc aggcgcggtc ggctttgagg ggaacgtgcg
      361 agccctcatg accccagtgc ggtgcgacga tcacggcatc agccgcaccg cccgcccgca
      421 tccggcgtac ctcgtcgagg atcgccgcct tgttggcgta gcagttgagc acctgtccaa
      481 gcttgtcggg catgccgttg gtcgaatagg tgcaggcgag ccagccgatc ttcatgccgg
      541 ccgcagcaac aatcgtaccc cacggactca aggcatcacc gcgccggcgg gtgcccgagt
      601 aggcgagttc atgcgccgca agctcgtcga tggtgcggtc gatgccgagc ggtccgcgat
      661 cggccgcgtg attgttggcg gtcgagagca ccttgaagcc gctcgccgcg agatcgccga
      721 tcaccgaggg gtggtagttg aacacaagcg ctccgcgtga acggccatag acgataccgt
      781 cgagccgccg cccgggatcg tcgacggcga tgccgccgat ccgaacgccg cgcgccgcgg
      841 gcccctcgag attgccatag ctgaggtcgg caccttcgag cacaggcagc agcggcgccc
      901 agagcgcgcg gaacgagcgc cccggcgcga gcgcccgcgc ctgcacggca tcatggaaca
      961 ggagatcacc gaccgcggcc aaggtggcgc ggtcgcccgt cgcgcaacct gccgcaaatg
     1021 tcagcggcgc acttgcggtc gtggacgggg cacgcggccc tgagcgcgcc tccccggacg
     1081 tgctcgcggt cacggcgagc agcgcggcga gagtgcagat gccgacaaaa acacgcatga
     1141 atgttgcgta ccaggacgag accgccgcgc caagcgagtt gcaggcacat ggcatcaacc
     1201 acgcctccag cggcgatgcg tcacgccgag cttgcggcgc cgatggttaa gacttcgcgc
     1261 ctcatttcat tggcctggag cgtgccttgg agccgatggt cttcctcgct gtcgtgctcg
     1321 cggccatcat gcacgccggc tggaatgccg tgatcaaggg tggcggcgat cccttcgcga
     1381 cgctcacgca catgtcgatg atgaacgccg tgatctcgct cgccgtgatc ccgttcgtcc
     1441 cctggcctcg gcccgaaaca tgggcctggc tcgtggcctc cgtcgtcatc cactcggcct
     1501 accggctgct gctgatcaat gcctatcgcc tcggcgacct cacccacgtc tatccgatcg
     1561 cgcgtggggc cgcgcccctc gtcacggcga tcgcgacttt tgcgctcatc gccgagacca
     1621 tcgctccgac cggctacatt gcaatcgccc tcctgtcgct cggggtcgtg ctcatgtcgc
     1681 tcaagggcgg ccggctcggc aatctcgacg gcaaggtgac gggcttcgca ttgctctcgg
     1741 ccgcttcgac gtcagcctac acgctcgtcg acgggcttgg cgcgcgcgcc aacggttcag
     1801 gcatcggctt cgcagtctgg atgttctttc taaacgcact cgccatgcaa gtgatcgccg
     1861 gattctggcg cggcccggcc gtatggacgt ccgttgccgg cgcctggcgc cccgcgctcg
     1921 cgggctccgc gctctcgatg ggggcttacg ctatcgtcat ctgggcaatg acactggcgc
     1981 cgatcgcact cgttgcttcg ctccgcgaga cgagcgtgct gttcgccgcg ctgatcggca
     2041 tcatcgtcct cggcgagccg gtgacgcgct ggcgcattct cgccgcgctc gcgatcggcg
     2101 ccggcatggt tctcatcaag gcgtcatgag cgatccggcc ccgagcgccg cccctgccga
     2161 cgtgccatgg cgcacgatct ggctgcttgc gctcgtctgc tttgcgagct cggcgaattt
     2221 gcggatagca gatgctctgc tcgcgcaggt cgccgaggat ttccgcgtga cggtcggcca
     2281 ggcctcgagc atcgtcgccg cgttcgccgt ctcgtatggg ctcctccaag ccgtgtacgg
     2341 cccggtcggc gatcggctcg gcaagctccg cgtcgtcgcc ttcggatcag cgctcgccgc
     2401 ggtcttcacc ggcgcctgcg cgatcatggg aagcctcgcc gctttgaccg ccgggcgctt
     2461 ccttgccggt gcggcggcgg cggcgatcat ccccctgtcg atggcctggg tcggcgaccg
     2521 cgtccccttc gagcgccgcc aacccgtgct cgcgcgattt ctgaccgggc agatgtcggg
     2581 actgatcttc gggcaggccg ccggcggcat cgtcggcgat ctcctcggct ggcgcggcgc
     2641 cttcgccatg ctggcgctcg tgcatgccgg cgcgaccgtc gtcctcgtcc agcacctcca
     2701 cgcgaccccc gggctcggcg cggcgagcag cgcaccggcc tccctccgcc agacgcttgc
     2761 cagcatacgc tcgatgctga cgcggagcta tccgcgcctc gtccttgcgg cggtcgcgct
     2821 cgaagcggcg gcgatgtacg gcgccttcgc ctatgtcggt gccgatctca aggcgcgctt
     2881 cggcatcggc tacgggctga tcggcctcat tctcggcacg ttcggcctcg gcgggctcat
     2941 ctatgcgttc cttgcgcccc gcctcctcgc cgtcatcggc gagcgcggct ttgccatatc
     3001 gggcggcctc gtgctcgcgg catcgtacct ggcgctcgcg tttgcggggt cgcttgccat
     3061 catcacagtg gccgtgctcg ccatgggctt cggattctac ctgctccaca acacattgca
     3121 ggtgaacgcg acgcagatgg cgcccgaagc gcgcggcctc agcgtcgcgg tgttcgcggc
     3181 tgtccttttc atgtcgcagt cggtgggcgt cgcgatggca gcgcccttca tcgacagcca
     3241 cggcgcgcga ccgatctatt tcgtcgcggc gctgctcttg ccgctgaccg gcctcctgct
     3301 cggcctccgc ctcgccaagg agcgcgctct ctcgattagg ccttgagtgc ggccagcacc
     3361 tcgtcgacga acgtcggcac gatctctgtc gccctgccgt gccggcactc gtggaagctc
     3421 gaggcgccgt ccgacggctc gaggttgagc tcgaccgtat aggcaccggc cgagcgcgcg
     3481 gtcgcgacga agcccgccgc ggggtagacg ttgccgctcg tgccgatcga gatgaagaga
     3541 tcggcgcgca tcaccgccca gctgatttcc tccatgtgat agggctgctc gccgaaccag
     3601 acgacgtcgg ggcgcatgcc gccggccttc ctgcatgttg ggcacgaaac gtcagcgccg
     3661 agatcgccca tccagggatg tcgcccgcgg cagttgcgac agagcgcctt caagagctcg
     3721 ccgtgcatgt ggacgacgcg ccgcgagccc gccgcttcgt gcaggttgtc gatgttctgc
     3781 gtgacgatcc ggaacgctcc cggccaggcc ttttcgaggc gggcgagcgc caggtgggcg
     3841 gcatttggtc tgacgccggc catgccctgc cgacgcttgt tatagaaatc ctggacgagg
     3901 ctcgggttgc gttcgaaggc ctcgggtgtc gccacgtcct cgatcctgac cttagcccac
     3961 agcccatccg catctcgaaa ggtatcgagg ccgctttcct tcgatatccc ggcgccggta
     4021 agcaccagga tgctcgactt cgaggtgagg ctcttcatgt gatgctccgc tcagggaatg
     4081 gtcgtggccg gcacgatgtc ggttgcggca aaatcggcgc ccttgaagag cagcggcgcc
     4141 ctctcgaggc ttgcgaacgc gtggctgcag caatcgccaa agttgaggcg ggtttccgag
     4201 ccggagctct tgccaaagcg ctcgtacgct gcaaccgcag attccgccat cgaacgatcg
     4261 aacggccgga tgatgatccg ggcgccaacg aaccactcat cgagtgcctg cacgcccgca
     4321 cggccacgcc tgccggacat gacgatcgac gcctccagca tggacacggc ggacatgatt
     4381 gcgtacgacg cctcactggt ccggcgagca aaagcatcgc gctcgggttc accttcgaga
     4441 atcgcgatga tggccgacgt atcgacgaca ttcatcgagg caatccggac tcgtcgtatc
     4501 cgatgatctc atcaggggtt cgtggataga gcagaggaag cgctgcaacg cgccccgagt
     4561 tttcccgagc gagttccatc acttcgccgg gccctacggc aaggcgcttg tcgacgccgg
     4621 ccgcacgcgc cgccgcctcg atggcctcgc gcacgacatc gtccggcgcc ctgccggttc
     4681 gcttggcaat gcgtcggacg aggccttcga tctcggccga gagttgcagc gtcatgggat
     4741 cggctccgac aaatcgaagc agatcatatc acaacagcgc agacttcacc cgatcttcaa
     4801 gcctgcggcg cgcatctcgg cgacgtagtc aacgccgtgt tcgatcggct tgccgtcgaa
     4861 gaaggcctcg aggttcttga acacgaaccg catcttggcc gtgaaggcgt cacgcgagcc
     4921 ggcggcgaca tgcggggtca ggacgacatt cgggaactgg tagagcgggt cgttcttcgg
     4981 tgcgggctcc acgtcgaata cgtcgaggcc ggcgccggcg atcttgcccg ccttcagcgc
     5041 cgcgacgagg tccggttcgt tgacgatgcc gccgcgggcg gcgttgatga ggaacgctgt
     5101 cggcttcatg cgctcgaaca ccttggcgtt gaacatgccc tgcgtcaccg gcgtccgcgg
     5161 cacgtggatg gtgacgatgt cgctgtccgc gatcagcttg tcgaagggca cacgcgtcac
     5221 ctcgagctcg cgttcgagct cgggcgggaa ggtcaggaga tcgtgataga ggatgcgcac
     5281 gccgaacggc ttcaggcggc gcgccaaccc cttgccgatg cggccaaggc cgacaatgcc
     5341 gatcgtcttg ccgccgatct cgtaggtgtt gtgccgcatc gaatagttgt ggaagcggcc
     5401 ggcccgcatc tcggcatcca tatagggcag ccgcttcatg gtcgcgagca tcaggaggat
     5461 cgtgtgctcg gcgacggcct gctccgtgcc cccgggcgtg atggcgagcg gcactttgcg
     5521 ctcagccagc gccgcgcaat cgaccgtatc gtgatagccg acgccctggt ggtggacgaa
     5581 cttgaggttt ttcgcggccg caatgcgctc acgtgtgagc ttggcgcccg cgacgatcac
     5641 cgcgtcggcc ctcgacaatg cctcgagccg ctcggcctcg tcgttgctct tgagcgtgat
     5701 cagctcgagc ccctgcggca cggcggcccg caccatctga taaaggtcct cggtgccgtg
     5761 gctgatgtag agaacgcgct tcttattgga cgatggacct gcggcgggct tggtcatgct
     5821 cggctctcga tcgggatggg cggcgcctgg attgacgaat gcttctggca gctccggtcc
     5881 ggcgaccgcc ggcctccgtt gcagcggcgc acgactatcg acacttgtcc cgcatcctgc
     5941 aagcgatccc tgaggccgat atcgcaaacc aagcatgcgc aggccacggc ttgccgccga
     6001 ggacgctacg gcctcacacc cgtcgatcgg gcctcgaccg ctggcacgag gccgagccgc
     6061 ctcacgcgtg ccgagacggc tgccacgctc cgggcatcga gcagctgctc acgcacgagg
     6121 atcgggcgtg catcgatctg caatggacgg agcctcgcaa gccgcgcgtc aatcgcctcg
     6181 tcagcaatcg gctcgccggc cccactgccc gaatagaaca gctccgtgat ggcaaaagcg
     6241 tctactgcca atcggatacg ccgatcatcg acgagctcgt cgccattctc gagcacgatc
     6301 agcagctcag gcttcagctg gcggacgtgg cttgcgatcc tgcgcacgag ggcagcgaga
     6361 tcatcacgcg ctcctggtcg ttcgagcgac cagctttgcg ccgcatcgcc gcccgtgagg
     6421 tagatgccgt cgactccgat ggcgacgagc cggttgatca gagagaacga gcgcccgatg
     6481 agccgggctt gccagtccga ctgccagaac cgcaccggaa acgtcgtcct gcgcacggca
     6541 ttctcgcgcg ccagccaggc cggcgcgcct gccgccggaa cgcggggaaa cgtgacgccc
     6601 gagcggctgc tgtcgcccgg cgagacatcg accacccagt ctgagcgcca catatcgctg
     6661 ctgtcggcaa tttcggcgag gctcacatgc gcaagcacga ccctgtcgcc gccaccggtt
     6721 cggtgccgca aggcctgcac gtcggcccgg ctcagcagct gcctgtctcg tgtggccggc
     6781 acgacgacca gatcgtcgcc agcgccgacc tccgccgtct gcccgacgtc gcggaatgcc
     6841 caactcgcga cgccggccag ggcgtcgttg cgcgcctcga cggcgggacc ttcggcttca
     6901 ggatcgatcg cagaatgcat gagccatgcg ccgcccgcga tcgcgagcgc caggacggcg
     6961 atgccgatcg ctgcccggcg caggaaccag cctgtcgagg cgggcgcccg cggcgcgact
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cgatgacgtc acggtgtcac ttcctcaatc
     7081 gtatccgccg ccggaactcg gtcgccgatc catccgcgcc gggaccgggc ggccaatcgc
     7141 gcgcttgcgc gcgcccttca tcagtgacgg aggagacggt attgaggctg agcacaggca
     7201 gggtcaaggc ggcagcagcg aattgccggg cattgtgcac gacagccaca cagcacgctt
     7261 caaccggcca catcccgggg cggatcgtca gcacatccgg ctatttgcgc gccgtccgcc
     7321 agaaaaaggc aactgcgagc agcggcagcc cgagactcga gcccagaacg ccgccgacga
     7381 gagccgacag attgtcgtac tgtccgcgct cgatcgggaa gagcaggttc aagtacttcg
     7441 tgagcagttg gctggccacg atcgcgaggc tcataagcga ggcggtgagc gcgaaccagg
     7501 tggcccgcgc tgcggccggg gcgtggaccg ctatcagcgt cagcagaggc acctgggcnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtcg aagagcgcga tgccgcgcgg
     7621 gccgagcccg aacatgtcat aggtccagcg atggccgccg ctcaccagca ggaggctcgg
     7681 cagccagagg attgcggcga caatcgtcag cgcgagcagc accaccgtca ccgtgcgccg
     7741 catcacggtg tcggtcagca gccacattgc gccgaggccg ataccggtac ctgtcacctg
     7801 cagcacaccg aaaaagcgct cgtcgaagcc gtgacggtcg atcaggaacc agcggtagcc
     7861 atcgccaagc gtcggcgcgg cgcgaaaggc gaagatcatg gccgccgcag ccgcgatccg
     7921 ccgcgcgatc gccggatcga gctcagccat tgcgagccgc gccatggcca ggatcacacc
     7981 gagcgcgatg agaaagaggg cctcctggcc gaagggcatc tccgagagcc cgagcgcgac
     8041 ggcgatcacg gcaaaggcaa gtccgccgcc gaggatccgc caatcgatcc ggcgcgcatg
     8101 ggctgcggcc agcacgttcg gcaggctcac gaccagcgcg ccgaggatcg agatcgccgg
     8161 cacggcgagc ccgatcagga agaccacgtg atagggtaag ctctcggcca gccgtcccgc
     8221 gagaaagccg acgaggaatg ccccaatcga gtaggtgagc cgcgagagta cctgcaccat
     8281 ggcaaggtcg ctgtcgacgt ctgcggggct gcgcggggcg ccgtccggcc gcacgcgggc
     8341 caccacctcc gcgctcaacg catccgcgac cacctcctgg atgatgccgc ctgcgaccac
     8401 cagcaactga gcgatcacgt aggcgcgttc aggcgggatt gccgtcaccg caccggaggc
     8461 ggcagcggcc agcagcagca gtcccgaggc gatgaggccc gcaccgagca aaatgtagac
     8521 gcgccgccgc gagcccatga tcgcgacgca gtcgacaacc tcgctcacca ccatcttgat
     8581 cgaccagggg agctgcagcc agacggcgag cgaggcgaga tcggcgggcg tgagcgtgag
     8641 cgacttcttc acccagaagc tgtcggcgac tgcaatgatg ccgagcgcgc ccgaggcgaa
     8701 atagaccatc agcagcggca agtacgcgag ccggaaggcg cgcgccggcg cgaggacgga
     8761 gtccgccgcg cgctcgatga cgccccggcg atccgtcggg atgttccccg ttgcgctcaa
     8821 agcgtcggac gcatcgagcg ggcgagctcg agcgtcgcca tgaagtcgac cttgcccgtc
     8881 ccgagtaccg
//
LOCUS       DAORNH010000191         8863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5856_length_8863_cov_2.705949, whole genome shotgun sequence.
ACCESSION   DAORNH010000191 DAORNH010000000
VERSION     DAORNH010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5856_length_8863_cov_2.705949"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..569)
                     /locus_tag="PK264_17145"
     CDS             complement(54..569)
                     /locus_tag="PK264_17145"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRD77634.1"
                     /translation="MSTRTKSRNFPAPEHNHAACLAGAHARAFAALEARGLRMTPIRE
                     KVFAEIAASHAAIGAYEVLDRLAAKGTRLAPISVYRALDVLVEAGVVHKLESQNAFFA
                     CHSPDEHSGPLARGAQLFLTCDTCGTVAEVAARPTEEAILAAAIAHRFTITRSVIEVR
                     GTCAHCVTTRR"
     gene            complement(644..1288)
                     /locus_tag="PK264_17150"
     CDS             complement(644..1288)
                     /locus_tag="PK264_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HRD77635.1"
                     /translation="MVNIPDKSRRAELGARVEHGVETVIFASRWILAPIYLGLAGGLL
                     ILLLKFLQEFWHLAVIALKASEADVILGILSLVDLAMTASLLIIVMFAGYENFVSRIA
                     HEGHRDWPTWMGTIDFAALKLKLLSSIVAISAIQLLKDFMSISKLPIAATPGVPSFDE
                     RRLFWSVVIHVVFVVSSLILALSDRVSGDHHGDPPPVPPAEPAVGSSSAPDAKP"
     gene            1541..2263
                     /locus_tag="PK264_17155"
     CDS             1541..2263
                     /locus_tag="PK264_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HRD77636.1"
                     /translation="MNAVKVLPLEKSVIDTLAGVSTATITTILLKKGLRNVWMRGTRP
                     LAAGQPRLVGRAFTLRFVPAREDLATPESWSSPVSTRAAIEAMPAGSIAVADAMGVTD
                     AGIFGDILCARMKKRGVAGLVTDGVVRDLVGVLGSKLPVWCQGGAAPPSVAGLTFVAW
                     QQPVGCGGVAVFPNDVVVVDDDGAVLIPQAHLADLLAAAPEQERMEGWIMGEVEKGAA
                     LPGLYPMNAETKARYEASKAKK"
     gene            2296..3630
                     /locus_tag="PK264_17160"
     CDS             2296..3630
                     /locus_tag="PK264_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD77637.1"
                     /translation="MNRPKPATAAETKAIIDRIEAENQRLKAFTHVLRADGIADAERA
                     DRLTKAGAGNGLVHGLPIVVKDIIDVAGVTSGCGPRAGAPPAVRDAHVVRRLRSEGAV
                     VVAKTHTVEYAFGGYGTNITVGTPWNPWDRKVHRIPGGSSSGTGVAVGAGLVPAGLGT
                     DTGGSVRTPASLCGCVGLKTSIGLVSRAGVAPLSQTLDTVGPLARDVTTAAYMLAAMQ
                     GHDPDDPSTDGVKPVDPLSGIEKGVNGLRLGVFQAVDLPGINPEVLADFGVAVKRLTD
                     LGALAKPLRLPQSLDAMTRGCGTIIASEAYANYRALVDDEASKLAAPIRNRMKAARNI
                     GAADLIQTRRERREWISALLAAIDGFDAVVLPTMAFPAIPVSDVDEAVSPLGTTRWVN
                     YLALAGLSVPTALSKGGLPMGLQIVVRRFDDPLALRIGRAFEAARGPFPLPQ"
     gene            3721..4632
                     /locus_tag="PK264_17165"
     CDS             3721..4632
                     /locus_tag="PK264_17165"
                     /inference="COORDINATES: protein motif:HMM:NF022061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRD77638.1"
                     /translation="MRKAVLVGAAVLVAGVAAVAAQDHRGRMGRGHGHGMGPMGDMGF
                     GPMGSGPMGGMRGMRGGPGMGLKALDTDKDGAVTLAEFLVPHETRFGEHDTNKDGSID
                     AAEAGARSKASVDYWMKVVMRRLDADRDGKVTKEEFRKRARDGFAMRDLDGDGQITDA
                     DLPPGMKGRMDAWRKHRDATAPDGKGSGDGKGPPDGKGPRSGLGGPFNLDRILGRSDA
                     EFARLDRNSDGVIDAKDLDVIAAERTEYWVKRFMHRYDQNKDGKASKDEFLRFARERF
                     AMIDLDGDGKITEADLPPMMRGRGILK"
     gene            4750..5379
                     /locus_tag="PK264_17170"
     CDS             4750..5379
                     /locus_tag="PK264_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRD77639.1"
                     /translation="MRLRLAETPKDGPSRRTGSSGDGTDEADLLRRVAGGDQQAFRTL
                     VRRHLPAILVSARRIVLDEAEAEDIAQEALLRLWRGAATLEVGQSGTRPWLRRVATNL
                     AIDRIRARRGTDVVDEPPEVPVAARQQSEIEETEMSAAVHRALAHLPERQRLALTLFH
                     FEGLSQIEVGRALGVSDEAVESLLARARRTLKGELQGQWRELLPGAPDI"
     gene            5451..5987
                     /locus_tag="PK264_17175"
     CDS             5451..5987
                     /locus_tag="PK264_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77640.1"
                     /translation="MLTIERLTFLLETYGTDRRRWPRGADQGLDAFLTTHAEARAHWT
                     EAAAFERVISAARAEPHLAEGRLTALEERIVAAAMAGDRGRADATMTTGNVIAWPGGR
                     GTAVAGPEPAQVVRQAVGASRRWGIGGLLAASLAIGVTLGVYDLAPAPQLLAGVSEPS
                     GAPVAVASFYVDPDEEAL"
     gene            5987..6556
                     /locus_tag="PK264_17180"
     CDS             5987..6556
                     /locus_tag="PK264_17180"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HRD77641.1"
                     /translation="MRVVAEGPDAAGPQPRGFFARWGKPLLVASLALNLVTVGLIAGA
                     KMRERRMWHAIGLAGAPGSEGRASAVAQLGSLAFVETLPKERRDSLLGAAKERWEAVK
                     ALRREARGARVELVRLMDTEPFDQSGAKAKAERIVEAESKARAAFHALLIEIVNGMTP
                     DERELYKVWRQKFRHPGAPGHGPRGAGGL"
     gene            6677..>7125
                     /locus_tag="PK264_17185"
     CDS             6677..>7125
                     /locus_tag="PK264_17185"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HRD77642.1"
                     /translation="MPQQDLKRAALDYHEADPPGKIAIHATKPVANQHDLALAYSPGV
                     AHACEAIAADPSAASRYTARGNLVGVITNGTAVLGLGDIGPLASKPVMEGKAVLFKKF
                     AGIDVYDIEVAEKDAEKFVEAVARLEPTFGGINLEDIKAPECFEIERA"
     assembly_gap    7126..7148
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7149..>8863
                     /locus_tag="PK264_17190"
     CDS             <7149..>8863
                     /locus_tag="PK264_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRD77643.1"
                     /translation="VFHDDQHGTAIVVAAGILNGLKVVGKRLEDVRLVCSGAGAAALA
                     CLNLLVSLGLKRQNVWIADIKGVVYAGRKELMDPYKEPFAQPTEKRTLAEIMPGADIF
                     LGLSAGRIVSKEMVAAMAAKPLIFALANPTPEIMPEDAREVRPDAVIASGRTDYPNQI
                     NNVLCFPFIFRGALDVGATTINEAMKKAAVTALASIAESEASDVVLNAYRAETFVFGP
                     DYILPKPFDPRLITEIAPAVAKAAMETGVATRPVADLAAYRARLTGFVYRSGFLMKPL
                     FDLARSASAETRQRLVFAEGDHPYVLQAAQQIISEGLARPILVGRRAHITRQLPHLGL
                     RMRPEQDFDIFDAETDKRIGQLEAEYQALVERKGVSPSHARRVVRNGSTVIAGLLLKR
                     GDADAVICGAVGRYLTQLRHVGQVIGRMPGVRGFATLSATFMPTGPLFIADTYVSYDP
                     SAEQIAEMTLLAVDEVRRFGVTPKVALLSHSNFGSEDTASARKMREVLSILRDWSPEL
                     EVEGEMHADAALSPFIREEIFPSSRLSGAANLLIMPGLDAANIAFNLLKVISGGVAVS
                     PILLG"
BASE COUNT         1463 a         2910 c         3059 g         1408 t
ORIGIN      
        1 agagtggcag gcggtgcatc gggggcttat ccggtttgat ctcgatcgtc ggttcagcgg
       61 cgggtggtaa cgcaatgcgc acaagtgccg cgcacctcga tcacactgcg ggtgattgtg
      121 aaccgatgtg cgatggcggc ggcgaggatg gcctcttcgg tcgggcgcgc cgcgacctcg
      181 gcgacagtgc cgcaggtatc gcaggtgagg aacagctgcg cgccgcgggc gagggggccg
      241 gagtgctcgt ccgggctgtg gcaggcgaaa aacgcgttct gcgattcgag cttgtgcacg
      301 acacccgcct cgaccagcac gtcgagcgcc ctgtagaccg agatcggcgc gagccgcgtt
      361 cccttggcgg ccagccggtc cagcacctcg taggcaccga tcgcggcatg ggaggctgcg
      421 atctcggcga acaccttctc gcggatcggt gtcatgcgca gcccgcgcgc ttcgagcgcg
      481 gcgaacgccc gggcgtgcgc cccggcgagg catgccgcat gattgtgctc gggtgccggg
      541 aagttccggc tcttggttcg tgtcgacatg ataatctctg tttggtctcc atggctttgc
      601 aaggaccggc tacggcgtca aggcgcgccc gacagcgagg tgctcagggc ttggcgtcgg
      661 gagcgctcga ggagccgacc gcaggctccg ctggcgggac cggcggcgga tcgccgtgat
      721 ggtcgcccga gacgcggtcc gagagcgcga ggatgaggct cgagacaacg aacacgacgt
      781 ggatcacgac cgaccagaag aggcgccgct cgtcgaagga gggcacaccc ggtgtcgccg
      841 cgatcggcag cttggagatg ctcatgaagt ccttgagcag ctggatcgcc gagatggcga
      901 cgatcgagga gaggagcttc agcttcaacg cggcaaagtc gatcgtgccc atccaggtcg
      961 gccagtcgcg atggccttcg tgcgcgatcc gcgagacgaa gttctcgtac ccggcgaaca
     1021 tcacgatgat cagcagcgag gccgtcatgg cgagatcgac gagcgagagt atgccgagaa
     1081 tgacgtcggc ttccgaagcc ttgagcgcga tcacggcgag gtgccagaac tcctgcagga
     1141 acttcaggag caggatgagc agaccgcccg cgaggccgag gtagatcggc gccaggatcc
     1201 agcggctcgc gaagatcacg gtctcgacgc cgtgctctac cctggcgccg agttcggcgc
     1261 gccggctctt atctggaatg ttcaccacgt tgcgctccgc tcgatgctcg cttagccgcc
     1321 gccatcgcat gactttcctg gcacggcaac atgtgagaag cgcgtggaag agccgggctg
     1381 ccgcagcctg cacggcagca atgtcgtcga tcttccgtct gtccgccgcc tgtcgcgacc
     1441 gctaaggttg cgctaatcct gggtcgagac acctatgtgt cgctcgaccg ccagccaacc
     1501 ggagcacccg atgtccgccg acaaagccgc cgccgatgcc atgaacgccg taaaagttct
     1561 gccgcttgag aagagcgtca tcgacacgct cgcgggtgtc tcgaccgcca ccatcaccac
     1621 gatcctgctg aagaagggcc tacgcaatgt gtggatgcgc ggcacgcggc cgctcgccgc
     1681 cggccagccc cgcctcgtcg ggcgcgcatt cacgctccgc ttcgtccccg cgcgcgagga
     1741 tctggcgaca cccgaatcct ggtcgtcccc tgtttccacg cgcgccgcca tcgaggcgat
     1801 gcccgcgggc tcaattgccg tggccgatgc catgggtgtc actgacgccg gtatctttgg
     1861 cgatatcctc tgcgcgcgca tgaagaagcg gggcgtcgcg ggcctcgtta ccgacggcgt
     1921 ggtgcgcgat ctcgtcggcg tgctcggctc gaagctcccc gtgtggtgcc agggcggggc
     1981 ggcaccgccg tcggtcgcgg gccttacatt cgtcgcctgg cagcagcccg tcggctgtgg
     2041 cggcgtcgcc gtcttcccga acgatgtcgt cgtcgtcgat gacgacggcg ccgtgctcat
     2101 cccgcaggcg catctcgccg atctcctcgc agccgcgccc gagcaggagc gcatggaggg
     2161 ctggatcatg ggcgaggtcg agaagggcgc cgcactcccc gggctctatc cgatgaatgc
     2221 ggagaccaag gcgcgctatg aggcgagtaa ggccaagaag tgagcaatcc gatcacacgg
     2281 gagcaggagg ccgccatgaa tagacccaaa cccgcgacag ctgccgagac caaggccatc
     2341 atcgatcgga tcgaggccga gaaccagcgc ctcaaggcgt tcacgcatgt cctgagggcc
     2401 gacggcattg cagatgccga gcgcgccgac cgcttgacca aggccggcgc cggaaacggc
     2461 ctcgtccacg gcctgccgat tgtcgtcaag gacatcatcg atgtcgccgg cgtgacgagt
     2521 ggctgcggcc cccgcgccgg agccccgccc gccgttcgcg acgcccacgt cgtccgacgc
     2581 ctgcgcagtg aaggcgcggt ggtggtcgcc aagacgcata ccgtcgagta tgccttcggc
     2641 ggctacggca ccaacatcac cgtcggcacg ccctggaatc cgtgggaccg aaaggtgcat
     2701 cgcatcccgg gtgggtcgtc gtcggggact ggcgttgcgg tcggcgcagg cctcgtgccg
     2761 gcggggcttg gcacggacac gggcggctcg gtcaggacac cggcgagcct ctgcggctgc
     2821 gtcggcctca agacctcgat cgggctcgtg tcgcgggccg gcgtggcacc gctctcccag
     2881 acgctggaca cggtcggtcc gctcgcccgc gacgtgacga cggctgccta catgctcgcg
     2941 gccatgcagg gtcatgatcc ggacgatccg agcacggacg gcgtgaagcc ggtcgacccg
     3001 ctttcaggga ttgagaaggg tgtaaacggt ctgcggctcg gcgtcttcca ggccgtcgac
     3061 cttcctggca tcaacccgga ggtgctcgcg gattttggcg tggccgtgaa gcggctcacc
     3121 gatctcggcg cgctcgcaaa gccgttgcgg ctcccgcaat cgctcgatgc gatgacgcgg
     3181 ggctgcggca cgatcatcgc gtcggaggct tatgcgaact accgcgccct cgtcgatgac
     3241 gaggcctcca agcttgcggc accaatccga aaccgcatga aggcagcacg caacatcggc
     3301 gctgccgacc tcatccagac ccggcgcgag cgccgcgagt ggatttcggc gctgctcgcg
     3361 gcgatcgatg gctttgatgc tgttgtcctg ccaaccatgg cattcccggc gatccctgtg
     3421 tcggacgtgg acgaggcggt ctcgccgctc ggcacgacgc gctgggtcaa ctacctcgca
     3481 cttgccggtc tctccgtgcc gacggccttg agcaaaggcg gtctgccgat ggggctccag
     3541 atcgtggtgc gccgtttcga cgaccccctg gctctgcgca tcggacgggc tttcgaggcg
     3601 gcccgaggac cgttcccgct ccctcagtga ggcgcgacgg atcgccggaa ccggcccgtt
     3661 cttagtctcg aacgggccgg cgcgcgatgc gatccggtca gcctctcaag ggagtagcag
     3721 atgcgaaaag cagtgttggt gggcgccgcc gtgctcgttg cgggcgtagc ggcggtcgcg
     3781 gcccaggatc accgtggcag gatgggccgc gggcatggac acggcatggg cccgatgggc
     3841 gacatgggtt ttggcccgat gggctccgga ccgatgggcg gaatgcgcgg catgcgtggc
     3901 ggccccggca tgggcctcaa ggccctcgat acggacaagg acggcgcggt gacgctcgcc
     3961 gagtttctcg tcccgcacga gacccgcttc ggcgagcacg acaccaataa ggacggcagc
     4021 atcgacgcgg ccgaggccgg tgcccgatcc aaggcgagcg tcgactactg gatgaaggtc
     4081 gtcatgcgcc gcctcgacgc agaccgcgac ggcaaggtca ccaaagagga gttccgcaag
     4141 cgtgcccgtg acggcttcgc gatgcgtgac ctcgacggcg acggccagat cacggacgcc
     4201 gacctgccgc ccgggatgaa gggccgcatg gatgcctggc gcaagcaccg cgatgcgacg
     4261 gcacccgacg gcaagggctc gggcgatggc aagggtccgc ccgatggcaa gggtcctcgg
     4321 tccggacttg gcggcccctt caatcttgat cgcattctcg gccggtcgga cgccgagttc
     4381 gcccgcctcg accgcaacag cgacggcgtg attgatgcca aggacctcga cgtcatcgcg
     4441 gccgagcgca ccgagtactg ggtgaagcgg ttcatgcacc gctatgacca gaacaaggac
     4501 ggcaaggcca gcaaggacga gttcctgcgt tttgcgcgcg agcgcttcgc catgatcgac
     4561 ctcgacggcg acggcaagat cacggaggcg gacctgccgc cgatgatgcg cggccggggc
     4621 atcctgaagt aaggttgcgc ggagtggcgg gcggcctgtg agggccgcct gcgacccgcc
     4681 gcccttcccg aggagactga tgccgattga gcggcgttgg tcgccggagc cagcagggag
     4741 cggccagaga tgaggttgag attggccgag acgccgaagg atggtccgtc acggcggacg
     4801 ggatcgtcag gggacggtac ggacgaggcc gacttgctgc gtcgcgtggc cggcggcgat
     4861 cagcaggcct tccgcaccct ggtgcggcgt cacctgccag cgatccttgt gagtgcgcgt
     4921 cgtatcgtgc tcgacgaggc ggaggcggag gacatcgccc aggaggcgct gcttcgactt
     4981 tggaggggcg ctgcaactct cgaggtcgga caatccggta cccggccctg gctccggcgt
     5041 gtcgccacga acctcgcgat cgatcgcatc cgcgcgcggc gcggcacgga cgtggtcgac
     5101 gagccgcccg aggtgccggt cgccgcgcgc cagcagtccg agatagagga gaccgagatg
     5161 tcggccgccg tccatcgggc gctcgcccat ctccccgagc gacagaggtt ggcgctgacc
     5221 ctgttccact tcgagggcct gagccagatc gaggtgggaa gagccctcgg tgtgtcggac
     5281 gaagccgtcg aatcgctgct cgcgcgggcc cggcgcacac tcaagggcga gctccaaggc
     5341 caatggcgtg agctgttgcc aggggcgccg gatatctaga cggacacctg caccttgccg
     5401 agacgcctga actggccgca aatgccgccg ctgatcgaga gacgaccgac atgctgacga
     5461 tcgaacgcct gaccttcctg ctcgagacgt acggcacgga ccgtcgccgc tggccgcgcg
     5521 gagcggacca gggtctcgac gccttcctca cgacccacgc cgaggcccgg gcacactgga
     5581 cggaggctgc agccttcgaa cgcgtgatca gcgcggcccg cgcggagcca catcttgccg
     5641 aaggccggct caccgcgctc gaggagcgga tcgtggccgc cgccatggct ggtgaccgcg
     5701 gacgcgcgga cgccacaatg acaactggca atgtgatcgc ctggccgggc ggtcgaggca
     5761 ccgcggtcgc gggaccagag ccagcacagg tggtccgaca ggccgtcggc gcctcgcgac
     5821 gctggggcat tggtggcctg ctggccgcgt ccttggcgat cggtgtgacg cttggcgtct
     5881 acgatctggc gcccgcgccg cagctgctgg cgggcgtgtc tgagccgagc ggggccccag
     5941 tcgcggtcgc gagtttctat gtcgatccgg atgaggaggc gctctgatgc gggtcgtcgc
     6001 cgaggggccg gatgctgcag ggccgcaacc acgtggcttc tttgcgcgct ggggcaagcc
     6061 cctgctggtc gcgtctctgg ccctcaatct cgtgacggtc ggtctcatcg ccggcgccaa
     6121 gatgcgcgag cggcgcatgt ggcacgccat cgggctggca ggtgctcctg gatccgaggg
     6181 tcgggcgtct gccgtggcac agctcggcag cctcgccttt gtcgagacac tgccgaagga
     6241 gcggcgcgat agcttgctcg gcgcggcaaa ggagcgctgg gaggcggtca aggcactcag
     6301 gcgcgaggca cgaggcgccc gtgttgagct cgtccgcctg atggatacag agcccttcga
     6361 tcaatccggc gcgaaggcga aggccgagcg catcgtcgag gccgagagca aggcgcgggc
     6421 ggcattccac gcactactca tcgagatcgt gaacggcatg acgcccgatg aacgcgaact
     6481 ctacaaggtc tggcggcaga aatttcgcca ccccggcgcc cctggacacg ggccacgcgg
     6541 ggccgggggc ctctgatgta cgcgggcttc gagtgcttcg gagctttcaa tcccgcgccg
     6601 caccgcgcta gattggccgg gggcggcttg cgcagggcaa ggcgccggcc ttaggcccgg
     6661 aaccgcggat tgcgccatgc cacagcagga cctgaagcgc gcagcactcg actatcacga
     6721 agcagacccc cccggaaaaa tagccatcca cgcgacgaag cccgttgcga accagcatga
     6781 tctggcgctc gcatactcgc cgggcgtcgc gcatgcctgc gaggcgattg ccgccgaccc
     6841 ttctgctgcg tcgcgctata cggcgcgcgg aaacctcgtt ggcgtgatca ccaacgggac
     6901 ggcggtgctc ggtcttggcg acatcgggcc gctcgcgtcg aagccggtga tggaggggaa
     6961 agccgtcctc ttcaagaagt tcgccggcat cgacgtctac gacatcgagg tcgcagagaa
     7021 agacgcggag aagttcgtcg aggcggttgc ccggctcgag ccgaccttcg ggggcatcaa
     7081 cctcgaggac atcaaggcac cggaatgctt cgagatcgag cgggcnnnnn nnnnnnnnnn
     7141 nnnnnnnncg tcttccacga cgaccagcac ggtactgcga tcgtcgttgc cgccggcatc
     7201 ctgaacggtc tcaaagtcgt gggaaagcgc ctcgaggacg tccgcctcgt ctgctcgggc
     7261 gcgggtgccg cggcgctcgc ctgcctcaat ctcctcgtga gcctcggcct caagcgccag
     7321 aacgtgtgga tcgccgacat caagggtgtc gtctacgccg gccgcaagga gctgatggac
     7381 ccctacaagg agccgttcgc ccaaccgacc gagaagcgca cgctcgccga gatcatgccg
     7441 ggtgccgaca tcttcctcgg cctttccgcc ggccggattg tctccaagga gatggtcgcc
     7501 gcgatggcgg caaagcccct catcttcgcg ctcgccaacc cgacgcccga gatcatgccg
     7561 gaggatgcgc gcgaggtgcg cccggacgcc gtgattgctt caggccggac ggactatccc
     7621 aatcagatca acaacgtgct ctgcttccct ttcatcttcc ggggggctct cgacgtcggc
     7681 gcgacgacca tcaacgaggc gatgaagaag gcggccgtca ccgcgctcgc ctcgattgcc
     7741 gagagcgagg cctccgacgt cgtgctgaac gcctaccggg cagagacgtt cgtgttcggt
     7801 cccgactata tcctgccgaa gccgttcgat ccgcgcctca tcaccgagat cgcgccggcc
     7861 gtcgcaaaag ccgccatgga gacaggggtc gcgacgcggc cggtcgccga tctcgccgcc
     7921 tatcgggcgc ggctcacggg gttcgtctat cgatccggct ttctcatgaa gccgctgttc
     7981 gatttggcgc gcagcgcgtc ggccgagacg cgccagcggc tcgtctttgc cgagggcgat
     8041 cacccgtacg tgctgcaggc cgcgcagcag atcatctcgg aggggctcgc ccggccgatc
     8101 ctcgtcgggc ggcgcgctca catcacgcgg cagcttcctc atctcgggct ccgcatgcgg
     8161 cccgagcagg acttcgacat cttcgacgct gagaccgaca aacgcatcgg ccagctcgag
     8221 gccgagtatc aagcactcgt cgagcggaag ggcgtgtcgc cctcgcatgc gcgccgcgta
     8281 gtacgcaacg gctcgaccgt gatcgccgga ctccttctga agcgaggtga tgcggatgcc
     8341 gtaatctgcg gcgcggtcgg gcgctacctc acgcagcttc gccacgtcgg gcaggtgatc
     8401 gggcggatgc cgggcgtgcg cggcttcgcg acgctctcgg cgactttcat gccgacaggg
     8461 ccgctgttca tcgccgacac ttacgtctcc tacgatccga gtgccgagca gatcgccgag
     8521 atgacgctgc tcgccgtcga cgaggttcgc cgcttcggcg tgacgcccaa agtggcgctg
     8581 ctctcccact cgaatttcgg cagcgaggat acggcaagcg cgcgcaagat gcgcgaggtg
     8641 ctctctatcc tgcgcgactg gtcgccggag ctggaggtcg aaggcgagat gcacgcagat
     8701 gcggcgctgt cgccgttcat ccgcgaggag atcttcccga gttcgaggtt gtccggcgcc
     8761 gccaacttgc tgataatgcc ggggctcgat gcggccaaca tcgccttcaa tctgttgaag
     8821 gtcatatcgg gcggtgtcgc ggtgagcccg atcctgctcg gtg
//
LOCUS       DAORNH010000192         8863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5857_length_8863_cov_2.645209, whole genome shotgun sequence.
ACCESSION   DAORNH010000192 DAORNH010000000
VERSION     DAORNH010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_8863_cov_2.645209"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK264_17195"
     CDS             <1..315
                     /locus_tag="PK264_17195"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRD77644.1"
                     /translation="FGPMLAIGLGGIHVEVLKDVAILPAASGAHEVKCAIAGLRGAKL
                     LDGVRGAPPSDVAALIDVIVRLGQFAVDHADLIAEIDLNPVIVHEAGKGVTLADALIV
                     TR"
     gene            complement(325..>733)
                     /locus_tag="PK264_17200"
     CDS             complement(325..>733)
                     /locus_tag="PK264_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRD77645.1"
                     /translation="GWRSSAEGFVMRFATIRPLAAAALLAVAGAGLTACAGSGPSWNE
                     GPGWRLSGSPTTIPPPKPATPAAALALAPPPAPHAAPARKAGQSVAIVVQPGDSLTGL
                     AIRHRTSISAIMAANRMSDPRIVPGQQLIIPGK"
     assembly_gap    734..888
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1150..1605
                     /locus_tag="PK264_17205"
     CDS             1150..1605
                     /locus_tag="PK264_17205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRD77646.1"
                     /translation="MAAIVVALPPAGFWKNQAAYETAFGNTWRIVAASMFAYFCGEFV
                     NSFVLAKMKVWMEGRALWLRTISSTVFGEAVDSALFYPLAFYGTGIIPNEALPKVMLA
                     QFILKTLVEVAFTPFTYWIVGFLKRAEGFDHYDRDTDFNPFHVSDAKQS"
     gene            1614..>1869
                     /locus_tag="PK264_17210"
     CDS             1614..>1869
                     /locus_tag="PK264_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HRD77647.1"
                     /translation="MAAVIRKIVVTVEETHREAGITIAPATRKAAAIAVIENPFAGRR
                     VEDLEALVAIGEELGGLLTRRAVAALGIEGGKAESYGKAAI"
     assembly_gap    1870..2008
                     /estimated_length=139
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2009..2189
                     /locus_tag="PK264_17215"
     CDS             <2009..2189
                     /locus_tag="PK264_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HRD77648.1"
                     /translation="GHKDAAFVRSHFDAMEVRVVDAPRSNEILVAIVVTDSGRPQPRI
                     GGLTTSEIKGTDGLR"
     gene            complement(2202..3434)
                     /locus_tag="PK264_17220"
     CDS             complement(2202..3434)
                     /locus_tag="PK264_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77649.1"
                     /translation="MAETVTPSNEWGSEQRSRRVNAFHAPYRRIIADFTSREKALEDL
                     AESFPALLFALATGYGPLEPRTAATAMVLAGRSLREAAAMAGLPLWMKRLPPGAFMRP
                     LLPVPNVPSLASLLGSSLTRNAQMAAIWLDRLLLAHAIAGVPAAEWMARTGMRMHAHE
                     TSCPLPSLMAWIWASANRATELGALIEQPWHPTLSSGQCVERATAWRRHIDLACAIPH
                     RIGDAWVKEGPCGEFEVRALRTPRDIIKAARDLDNCLVTWGDRIGYSCSRVFALCRNG
                     VTVAAFEIVHDSDDPPVPRLSQILGYSNRQVSTEIRNTVLAWLGEQAFRRYDTQPISP
                     ALQLARQDELLRPFFQQLLQIPGCDAELVERFRRELRRDPPTERMRRQIEYERRLLNE
                     FTIRRQAYLNLRQLLRPT"
     gene            3677..4141
                     /locus_tag="PK264_17225"
     CDS             3677..4141
                     /locus_tag="PK264_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD77650.1"
                     /translation="MLASIARAANGAAGGLAEIDPLELSDVLGTPHVIVLDCRSTEEH
                     EVSHIAGAHRVDPDWPRLPRALDDESLVVCACAVGLRSAAVAAELIKQGLVRRESCRN
                     LAGGLFAWHNAGLPLVDASGPAARIHAYSRLWLPLLERRELSVLGRSGSVTG"
     gene            4156..4743
                     /locus_tag="PK264_17230"
     CDS             4156..4743
                     /locus_tag="PK264_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77651.1"
                     /translation="MVQRLDRAIVAAIGCFLIAGAATAEDPRLKTRLAATSVAEKSRT
                     LAGLYLTAKEASSAIRRYPDLLLVDVRFPRVVAEGLPAPVTRNIPIYREVVHRKGEID
                     ELVEQSLNPEFLSAVEALASGRRGKATTVLLICWAGPYSAVAADHLTRAGFANVYTVI
                     DGTDGTDEPTLPGWRKSGQPWLEEPRPDQIWVAHD"
     assembly_gap    4796..4827
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4828..6031
                     /locus_tag="PK264_17235"
     CDS             <4828..6031
                     /locus_tag="PK264_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77652.1"
                     /translation="SGEPAWFENAGPRRRASGSLAMVAGRALSALSFAAALGMTVSAA
                     VAGEIISGPAGEPVHAQLCPRLESELKDARFRHACATSSGAVETIARIARSPLSVGFV
                     PLDQLAHASRGGVELTGLTILGRSDARVCLFIATRSSTLQSYGDIAAQIGKVRVILPP
                     RSSAGAEAFAYLQSIDPDGLGKARVISHAASAEDAVKEAMSADDIVTLVSEWPDPESP
                     RLKAVLEADGRFVAFADRTLLRKEVLGRRIYLPVEVEVTPATWTKAARKVATVCSPIA
                     IVAAAQSVVPERDRQDHLDLIATIETMRPEALTPRDGRVARYMKSTRDLTGASVEELI
                     RLSEEARVKAQPYVDKAIAAGKQAVEAAKPHVEKAKDIGAEALAKAREDFKALMDGLR
                     RDTTPRKE"
     gene            6047..6922
                     /locus_tag="PK264_17240"
     CDS             6047..6922
                     /locus_tag="PK264_17240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014760938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRD77653.1"
                     /translation="MPDVSTLSWSEPTARLAAFISVFVLMAALELAWPRRRLGWSKAK
                     RWVTNLSIVGLGAALVRVFSAIAALTAVPIVATLAAAWAEARGIGLLNMVAWPAGLEV
                     LFAVVILDFAIWLQHLLAHRIELLWLFHRMHHADVDFDVTTALRFHPVEIGLSMLYKV
                     VWVVALGASPLAVLIFEVVLNALAMFNHANFALPLPIDRMMRGLIVTPDMHRVHHSIS
                     PREHHSNFGFNLSVWDRLFGTYNAQPAAGHEAMTIGLPPWQSEAPTGLGWSLKLPFTG
                     PAPDHKVRAMSARRD"
     gene            7024..8679
                     /locus_tag="PK264_17245"
     CDS             7024..8679
                     /locus_tag="PK264_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD77654.1"
                     /translation="MTRSADTPLRGADLVARALIDAGAGPIYTLSGNHIMSIFDAAVG
                     KPLELIHTRHEAATVHMADAYGRLTGKPGIAMVTGGPGHANAVGALYTALCAESPMVL
                     LSGHAGTDEIGRGGFQEIRQAEMAAPVAKASWTAASAEGLGADVSRAMALAVYGRPGP
                     VHVSLPSDLLDHAVALTPGRVSPGVPSAARAGAPSGVVVDAILAELGRAERPVIIAGP
                     MFSHAGRRSLLARLEAATGVPVVLMEAVRGLRDATIGAFGDALGEADAVLLLGKPLDF
                     TLKFGAVPTLGATCRVLMVDPEAHIIGRAASELGDRLVLSTIAYGLAAADALISRAKP
                     SPKHAIWAGEVKRLVAHRPPAWATTRSKTPGRVHPLEVFRALAPVLERDADAVLVGDG
                     GEFSQWAQGALPVGRRMINSVAGAIGPGIPSAIAARTVERAAPVISIVGDGTFGFHMA
                     EFDTAVRHRLPFVCIVGNDARWNAEYQIQLRDYGPNRAIGCDLLPTRYDQVVTALGGY
                     GEMIDDAAKIPAAIERAIASGKPACLNVMTESIAAPNVRRSDL"
     gene            complement(8705..8781)
                     /locus_tag="PK264_17250"
     tRNA            complement(8705..8781)
                     /locus_tag="PK264_17250"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8745..8747),aa:His,seq:gtg)
BASE COUNT         1357 a         2780 c         2953 g         1447 t
ORIGIN      
        1 ttcggaccga tgctggcgat cggcctcggc ggcatccacg tcgaggtgct gaaggacgtg
       61 gcgatcctgc cggcggcaag cggcgcccac gaggtcaagt gcgcgatcgc gggcctgcgc
      121 ggagcgaagc tgctcgatgg ggtacgcggc gccccgccct cggatgtcgc agcactcatc
      181 gacgtgatcg tgcggctcgg ccaattcgcg gttgatcacg ccgatctcat tgccgagatc
      241 gacctcaatc cggtgatcgt gcacgaggca gggaagggcg tcacgctggc cgacgccctg
      301 atcgtcactc gctgatgggc gcgttcattt cccgggaatg atgagctgct ggcccggcac
      361 gatgcgtggg tccgacatgc ggttcgcggc catgatcgcc gagatggacg tgcggtgacg
      421 aatggcgagg cccgtcaagc tgtcacccgg ctgcaccacg atcgctacgg actggccagc
      481 ctttcgggcg ggggctgcat gaggagcggg tggcggcgcg agtgccagcg ctgcagccgg
      541 tgttgcgggc ttaggcggcg ggatggtcgt cgggctgccg gaaaggcgcc agccagggcc
      601 ctcgttccag ctcgggccgg agcccgcaca ggccgtgagg ccagcgccgg cgacggcaag
      661 cagggcggcg gcggccagcg ggcgaatggt ggcgaagcgc atgacaaatc cttcggcgga
      721 tgatcgccat ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cagcacccgc
      901 cccgcaacgc ccgctccgct atttcgacct ggtcatggtg gccttcgtcg tcgtgctggt
      961 ctgctcgaac ctcgtcgggc cggcaaagat cgcggaggtc aacctgccgc tgtggggcac
     1021 gttcgtattc ggcgcgggcg tgatgttttt ccccatctcc tacgtattcg gcgacatcct
     1081 gaccgaggtc tacggctaca gccgggcgtc atctggacgg ggttcggcgc gctcctcttc
     1141 gcctcggtca tggcggcaat cgtcgtcgcg ctgccgccag ccggcttctg gaagaaccag
     1201 gccgcctacg agaccgcgtt cggcaacacc tggcggatcg tcgccgcatc gatgttcgcc
     1261 tatttctgtg gcgagttcgt caactccttc gtgctcgcga agatgaaggt gtggatggag
     1321 ggccgggccc tctggctgcg taccatcagc tcgacagtgt tcggcgaggc tgtcgacagc
     1381 gcgctcttct atccgctcgc cttctatggc acgggcatca tcccgaacga ggcgctgccc
     1441 aaggtgatgc tcgcccagtt catcctgaag acgctcgtcg aggtcgcctt cacgcccttc
     1501 acgtattgga tcgtcggatt cctgaagcgc gccgagggct tcgaccatta cgaccgcgac
     1561 acggatttca atccgttcca cgtcagcgac gcgaagcaat cctgagaggt gccatggcgg
     1621 ccgtcatccg caagatcgtc gtgaccgtcg aagagacgca ccgcgaggcc ggcatcacga
     1681 tcgcgcctgc aacgcgcaag gcggcggcaa tcgccgtcat cgagaacccg ttcgccgggc
     1741 gccgggtcga ggacctcgag gcactcgtcg ccatcggcga ggaactcggc ggactgctga
     1801 cgcgccgcgc ggtcgcagcg ctcggcattg agggcggcaa ggcggagagc tacggcaagg
     1861 ccgcgatcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggcacaagga cgcggcgttc gtgcgcagcc
     2041 acttcgacgc catggaggtg cgcgtggtgg atgcacctcg ctcgaacgaa attctcgtgg
     2101 cgatcgtcgt gaccgacagt ggccgtcccc agccgcgtat cggcggtctc acgacgagcg
     2161 agatcaaggg gacggacggc ttgaggtagg catctcgcgg gtcacgtcgg ccgcaggagc
     2221 tggcgcaagt tcagataggc ttgcctgcgg atcgtgaact cgttcaagag gcggcgctcg
     2281 tactcaattt ggcggcgcat ccgctcggtt ggcggatcgc gccggagttc gcgccggaaa
     2341 cgctcgacaa gttcggcatc gcagccgggg atctgaagga gctgctggaa gaaggggcgc
     2401 aagagctcgt cctggcgcgc gagctgaagg gcgggagaaa tcggctgtgt atcatagcga
     2461 cggaaggcct gctcaccgag ccaggcaagt accgtgttgc ggatctccgt ggagacctgg
     2521 cggttcgaat agccgaggat ctggctcagg cgcggcaccg gcggatcgtc tgagtcgtgc
     2581 acgatctcga aggccgcgac agtgacgccg ttgcggcaaa gcgcgaacac tcgtgagcaa
     2641 ctgtagccga tcctgtcacc ccaggtgacg aggcaattgt cgaggtcgcg cgcggccttg
     2701 atgatgtcgc gcggggtacg tagtgcgcgc acctcgaact ctccgcacgg gccttccttg
     2761 acccatgcat cgccgatccg gtgcgggatg gcgcaggcga gatcaatgtg gcggcgccag
     2821 gctgtggcac gctcgacgca ttgacccgac gagagagttg gatgccatgg ctgctcgatg
     2881 agggcaccga gttcggttgc gcgattggcg ctcgcccata tccaggccat cagacttggc
     2941 agcggacacg acgtctcatg ggcatgcatc cgcattccgg tccgggccat ccattcggcc
     3001 gccgggacgc cagcgatggc gtgggcaagc agtagccggt cgagccagat cgccgccatc
     3061 tgcgcgttgc gtgtgaggct cgagccgagc agcgaagcga gcgaggggac attgggaaca
     3121 ggcagcagcg gccgcatgaa tgcgccgggt ggcagtcgct tcatccataa tggcaggccg
     3181 gccatcgcgg ctgcctctcg cagcgaccgg ccggccaaca ccatcgccgt ggcggcggtc
     3241 ctcggctcga gcggtccgta acccgtggcg agcgcgaaca gcagggcggg aaagctctcc
     3301 gccagatcct cgagtgcctt ctcacgtgac gtgaagtcgg cgatgatacg gcggtaaggc
     3361 gcatggaacg cgttgacgcg acgcgaacgc tgttccgatc cccattcgtt ggatggggtt
     3421 acggtctcgg ccaagtgatc cccccgagca ggcaacggtc atcaggcacc gaagaaatag
     3481 gatcgcatcc gccgtgttgc cagggtctcc gatggaatag atcgaaaaaa cgtatgaagg
     3541 ccgggatcat cccgagaacc cacgtcggag gaggcgcgca atgaccacga ccggggctgc
     3601 ccgcaggcga cgccggatcg tcgcgcgcat gctgccgcga ctggcgatcg cgccctggtg
     3661 gcgaggacga gggcaggtgc tcgcgtcgat tgcccgagca gcgaacggcg cagcgggcgg
     3721 cctcgcggag atcgatcccc ttgagctcag cgacgttctc ggcacgccgc atgtcatcgt
     3781 gctcgactgc cgctccacag aggagcacga ggtgagccat atcgccggcg ctcatcgcgt
     3841 cgatccggat tggccccggc tgccgcgcgc gctcgacgac gagagccttg ttgtctgcgc
     3901 ctgtgcggtc ggtctgcgct cagccgcggt cgcggcggag ctgatcaagc agggactggt
     3961 cagacgtgaa agctgccgca atctcgcggg cggtctcttt gcctggcaca atgcgggctt
     4021 gccgctggtc gatgcctctg gccccgcggc gcggattcac gcatattcgc gtctgtggct
     4081 tccattgctt gagagacgcg aattgagtgt gctcggccgg tcggggtcgg ttacgggctg
     4141 acaaggggcc gaaccatggt ccagagactg gacagagcga ttgtcgcagc catcggctgc
     4201 tttctgattg ctggagcagc cacggccgag gatccgcgcc tcaagacgcg gctggccgcg
     4261 acgtctgtcg ccgaaaaatc tcggacgctt gcgggtctct atctgacggc gaaggaggcg
     4321 tcttcggcca tccggcgata ccccgatctc ctgctcgtgg atgtccgttt tccgcgcgtc
     4381 gttgcagaag gcctcccggc gccggtgacc cgcaacattc ccatctatcg cgaggtcgtg
     4441 catcggaagg gcgagatcga cgagcttgtc gagcagtcgc tgaaccccga gttcttgtct
     4501 gccgtcgagg ccctcgcgag cgggcggcgg gggaaggcga cgacggttct gctgatctgc
     4561 tgggcggggc cctactcggc cgttgccgcg gatcacctga cacgtgcggg cttcgcgaac
     4621 gtctataccg tcatcgacgg tactgatggg acagatgagc cgacgctgcc cggatggcgc
     4681 aagagtggcc agccttggct cgaggagccg cgtccggatc agatctgggt cgcccacgat
     4741 tgaggcgcgt cacgccgctg cgattgagca ggtgcgcgtc gatgcgcgcc acctgnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gggcgagccg gcatggttcg agaacgcggg
     4861 cccgcggcgg cgcgcctctg ggagtttggc catggtggca ggacgcgcac tctcggcctt
     4921 gtcgtttgca gcagcgctcg gcatgacggt atcggcggcc gttgcaggcg agatcatctc
     4981 gggaccggcc ggcgagccgg ttcatgccca gctctgcccg cgtcttgaga gcgagctcaa
     5041 ggacgcgcgc ttccgccatg cctgtgcgac ctcgtccgga gccgtcgaaa cgatcgcgcg
     5101 gatcgcccgc agccccctgt cggtcggctt cgtcccgctc gaccagcttg cgcacgcgag
     5161 ccgcggcggg gtcgagctga ccgggctcac cattctcggc cgcagcgatg cccgcgtctg
     5221 cctgttcatt gcgacgcgaa gctccacgct ccagagctac ggcgacattg ccgcccagat
     5281 cggcaaagtc cgcgtcattc tgcccccgcg gagcagcgcc ggtgccgagg cgttcgcata
     5341 ccttcagtcg attgatcccg atggcctcgg caaggcgcga gtgatcagcc acgcggcgag
     5401 tgctgaagac gccgtcaagg aggcgatgag cgccgatgac atcgtgacgc tcgtttcgga
     5461 gtggccggac ccggagagcc cgcggctcaa agcggtgctt gaggcggacg gccgtttcgt
     5521 cgccttcgcg gaccgcacgc tgctgcgaaa ggaggtgctc ggccggcgca tctatctgcc
     5581 cgtcgaggtc gaggtcacgc ccgcgacctg gaccaaagcc gcgcgcaagg tcgcgaccgt
     5641 gtgctcgccg attgccatcg tcgcggcagc ccagtccgtg gtgcccgagc gcgatcgcca
     5701 ggaccacctt gacctcatcg cgacgatcga gaccatgcga cccgaggcgc tcacgccgcg
     5761 tgacgggcgc gtggcgcgct acatgaagag cacgcgcgat ctgacgggcg cgagcgtcga
     5821 ggaactcatc cgattgtcgg aggaggcgcg cgtcaaggcg cagccttacg ttgacaaggc
     5881 gatcgccgcg ggcaagcagg ccgtcgaggc ggcaaagccc catgtcgaga aggccaagga
     5941 catcggcgcg gaggcgcttg ccaaggcccg cgaggatttc aaggccttga tggatggtct
     6001 caggcgtgac acgacgcccc ggaaggaata gcaggcgagc gcacgcatgc ccgacgtcag
     6061 tactttgtcg tggagcgagc cgacggcgcg gctcgcggcg ttcatttccg tatttgtgct
     6121 gatggcggct ctcgagctcg cctggccgcg gcgccgtctc ggctggtcga aagcgaagcg
     6181 ctgggtcacc aatctgtcga tcgtcggcct aggcgctgca ctcgtgcgtg tcttcagcgc
     6241 cattgccgcg ctcacagccg tgccgatcgt cgcgacgctc gcagccgcct gggccgaggc
     6301 gcgcggcatc ggcctcctga acatggtcgc ctggccggcc gggctcgagg tgctcttcgc
     6361 ggtcgtcatc ctcgatttcg cgatctggct gcagcatctc ctcgcccatc gcatcgagct
     6421 gctctggctc ttccaccgca tgcaccacgc tgacgtcgat ttcgacgtga cgacggcgct
     6481 gcgtttccat ccggtcgaga tcgggctctc gatgctctac aaggtggtgt gggtcgtggc
     6541 gctcggcgcc tcgccgctcg cggtgctgat cttcgaggtt gtgctgaatg cgctcgccat
     6601 gttcaatcac gcgaattttg cgctgcccct tccgatcgat cgcatgatgc gcggcctcat
     6661 cgtgacgccg gacatgcacc gcgtccacca ctcgatctcc cctcgcgagc atcactcgaa
     6721 cttcggcttc aacctgtcgg tctgggatcg tctgttcggc acctacaacg ctcagcccgc
     6781 agcgggccat gaggccatga cgatcggctt gccgccctgg cagagcgagg cgccgacggg
     6841 gctcggctgg agcctcaagc tgccgttcac ggggcccgcg cccgaccaca aggttcgagc
     6901 catgtccgcc cggcgcgact gatctgccgc cggtgcggcg ggatcgcgag agcgttggca
     6961 ccagggccaa aggtcggcta ggtcttctga tgagacccga tcccatcgtc gctggaaccc
     7021 cacatgacca gatcagccga cacgccgctc cgcggcgccg acctcgtggc gcgcgcgctc
     7081 atcgatgccg gggcagggcc gatctacaca ctctccggca atcacatcat gtcgatcttc
     7141 gacgcggcgg tcggcaagcc gcttgagctc atccacacgc gccacgaggc ggcgacggtg
     7201 cacatggcgg atgcctacgg ccgcctcacg gggaagcccg gcatcgccat ggtgaccgga
     7261 ggccctggcc acgcgaacgc cgtcggcgcc ctctacacgg cgctttgcgc cgagagcccg
     7321 atggtgctgc tatccggtca tgctggcacc gacgagatcg gccgcggcgg cttccaggag
     7381 atccgtcagg ccgaaatggc cgcgcccgtc gccaaggcct catggaccgc ggcgagtgcc
     7441 gagggcctcg gcgcggacgt gtcacgcgcg atggcactgg cggtgtacgg ccgaccaggg
     7501 cccgtgcacg tgagcctgcc atccgatctc cttgatcatg ccgtggcgtt gacgccgggc
     7561 cgcgtctccc ctggtgtgcc ttcagccgcg cgcgcaggag cgccgagcgg cgtggtcgtg
     7621 gacgcgatcc ttgccgagct cggccgggcg gagcggccgg tgatcatcgc ggggccgatg
     7681 ttctcgcatg ccgggcgtcg ctcgctgctc gctcgtctcg aggccgcaac cggcgtgccc
     7741 gtcgtgctga tggaggcggt ccgcggcttg cgcgacgcga caatcggcgc tttcggggac
     7801 gcgctcggcg aggcggatgc cgtgctcctt ctcgggaagc cgctcgactt cacattgaag
     7861 ttcggtgccg tgccgacgct cggcgcgacc tgccgagtgc taatggtcga tccggaggcg
     7921 catatcatcg gtcgggccgc gagcgagctc ggcgatcgcc tggtgttgtc gacgattgcc
     7981 tatgggctcg ccgcggcaga tgcgctgatc tcgcgggcga agccgtctcc gaagcatgcg
     8041 atttgggcgg gcgaggttaa gcggctcgtg gcgcatcgcc ccccggcctg ggcaacgacg
     8101 cgatcgaaga cgccgggcag ggtgcatccg ctcgaagtgt tccgtgcgct ggcacccgtc
     8161 ctcgagcgtg atgccgacgc cgtactcgtc ggcgacggcg gcgagttctc gcaatgggcg
     8221 cagggagccc tgcccgtcgg ccgtcgcatg atcaactcgg tcgcgggcgc catcgggccg
     8281 gggattcctt cggcgattgc cgcgaggacg gtcgagagag ccgcgccggt gatctcgatc
     8341 gtcggcgatg gcaccttcgg cttccacatg gccgagttcg atacggcggt gcgacaccgc
     8401 ctgccgttcg tgtgcatcgt cgggaacgat gcacgatgga atgcggagta ccagatccag
     8461 ctccgcgact atgggcccaa ccgggcgatc ggctgcgatc tcctgccgac gcgctacgat
     8521 caggtcgtga cggcgctcgg cggctacggc gagatgatcg acgatgcggc gaagatcccc
     8581 gcggcgatcg agcgcgcgat cgccagcggc aaacccgcct gcctcaacgt catgacggaa
     8641 agcatcgccg ccccgaacgt gcgccgcagc gatctctgag cctggctcga gtgcttgatt
     8701 ttattggtac cgcctccccg gctcgaacgg gggaccccca gatccacaat ctggtgctct
     8761 aaccaactga gctaaggcgg cacttccgca cagtgatccg gccggagcac tgcggcgatg
     8821 tcgggcccgg ggacacggcg cgctgcgcaa gagcagagcg ccg
//
LOCUS       DAORNH010000193         8836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5877_length_8836_cov_2.681471, whole genome shotgun sequence.
ACCESSION   DAORNH010000193 DAORNH010000000
VERSION     DAORNH010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5877_length_8836_cov_2.681471"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PK264_17255"
     CDS             complement(<1..606)
                     /locus_tag="PK264_17255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD77655.1"
                     /translation="MQRGIGSGAVDAHAEVQDSLYSWGRLFVCLLLGAIGNIGMWSVV
                     VVLPTVQDAFGIARNGASFPYAMSMIGYAVGGYVMGRLADRVGIMIPILIGCVSLFAG
                     FILISQVTSIVQFAIVQGVMIGFFGSAAVFGPLMADISMWFERRRGLAVAMVASGNYI
                     SGTIWPPLIQRMVETIGWRETHVVMGILCLLIMLPLAFLLNR"
     gene            complement(635..2050)
                     /locus_tag="PK264_17260"
     CDS             complement(635..2050)
                     /locus_tag="PK264_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD77656.1"
                     /translation="MAGAAKLSTGGPQGDVATAIAVLRQRFPDRVQTGEAIRHQHSHT
                     LTWIASEPPDAVVWPHSTEEVAEIVRIAATHRVPIIPFGAGTSLEGHVNAPHGGISLD
                     FQQMNAVLAVNQLDLDATVQAGVSRKQLNDHLRDLGLFFPVDPGAEEATIGGMAATRA
                     SGTTAVRYGTMRDNVLGLTAVMADGSIVRTSRRARKSSAGYDLTRLLVGSEGTLGVFT
                     EVSVRLYGIPESIVAAVCPFETLEGAVNSVIQAIQLGLGHARSELLDEILIGAINKHA
                     KLGLEERPTLFLEFHGTEVSARDQIARFEEIAKAEGAIRFDWAEREEDRRRLWKARHE
                     GYWAIKSAYPGRDALATDVCVPISRLAECITETKADIEKTGLIASILGHVGDGNFHTQ
                     PMIDMSNPREVEATKGFVQRLTERAMRMDGTCTGEHGVGQGKIKYLEAEHGVGVEVMR
                     RIKAALDPDGLLNPGKILPER"
     gene            2326..3252
                     /locus_tag="PK264_17265"
     CDS             2326..3252
                     /locus_tag="PK264_17265"
                     /inference="COORDINATES: protein motif:HMM:NF016894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HRD77657.1"
                     /translation="MAEAELIGVDWGTTSFRAYLIGADGAVMARRESKAGILSVTNAA
                     FRETLVAELGDWARLHGARPIVMSGMIGSRQGWREAPYVPCPAGLREIAAALIEIDGG
                     PLGRIAIVPGLSIEADGIPDVMRGEETQIVGTLVTVPSEPRSIVLPGTHSKWVEVRDG
                     RITAFETYMTGEMFSAARDHTILGRLMGTTGADREAAFERGVEAGARSGAPGSLLHRL
                     FSARTLALFDRLPADDVASYLSGLLIGAELADRGPQAKPILIVASESLAEYYRRAAAV
                     IGLQAELAPPDCVAAGHRAIARQAGLIGDPSR"
     gene            3249..3866
                     /locus_tag="PK264_17270"
     CDS             3249..3866
                     /locus_tag="PK264_17270"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HRD77658.1"
                     /translation="MNLADALRQCPLIAILRGIRPDEAEPVGDVLVEAGFRVIEVPFN
                     SPEPLRSIERLARRLDKRAVIGAGTVMSIDDVEKVAAAGGRLIVMPHTDLDVVEAARD
                     AGLAVTPGVATPTEAIAAIAAGVDGLKLFPAEMMLPPVVKALRAVLPKEVILIPVGGI
                     SAANIPAYRAAGADAFGIGSTLYSPGKALADIRASAVALVKACRV"
     gene            complement(3873..4196)
                     /locus_tag="PK264_17275"
     CDS             complement(3873..4196)
                     /locus_tag="PK264_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HRD77659.1"
                     /translation="MSITVTSRAAARICDIGRAEAAPAILRVSVDGGGCSGFQYRFDL
                     VGASDPDDTIVDNGGAKVVVDPVSLPYLLGSEIDFVDDLIGAGFKIKNPNATASCGCG
                     TSFSV"
     assembly_gap    4445..4462
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4463..5546
                     /locus_tag="PK264_17280"
     CDS             <4463..5546
                     /locus_tag="PK264_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRD77660.1"
                     /translation="QRDRDRIIHATAFRRLTYKTQVFVFHEGDHFRTRLTHTLEVAQI
                     ARAIARQLEIDEDLTEALALAHDLGHPPFGHAGERALDRMLAGHGGFDHNAQSLAIVT
                     RLERKYPAFDGLNLSIETLEGLVKHNGPIAAALITPDRALGRVLAYSGVADRLSLDLF
                     APLEAQAAAIADDIAYNNHDVDDGLRAGLFTLDELTSVGLVGPLVEAAECKAIDRARR
                     IYEVNRQLITLMIADVVAEARSRISAHGCASVDDVRRAGRPIVEFSDGLKVGLADLKR
                     FLFTRVYRHPRVMRVMASAERIVEDLFRRYMAVPGDIPEPRRAEALALTEDGRALHVA
                     DFVAGMTDRFAIGEHRRLFDATPELV"
     gene            5600..7360
                     /gene="argS"
                     /locus_tag="PK264_17285"
     CDS             5600..7360
                     /gene="argS"
                     /locus_tag="PK264_17285"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRD77661.1"
                     /translation="MNVYSHLERAILSAVDGMKARGELPNELVASAGDIDPPRDPSHG
                     DLASNVAMVLAKAAKKKPRDIAEAIARQLAADPLIAKADVAGPGFLNIKLKPAAWHGV
                     VQTAVKAGPDYGRSDLGRGSAVNVEYVSANPTGPMHVGHCRGAVYGDVLASLLAFAGY
                     KVTREYYINDAGAQVGKLAQSTLLRYREALGEAIGEIPEGLYPGDYLKPVGAALAARF
                     GRKLLEKPLPEQLAEARAAAVSTMMAEIKDDLAALAVRHDVFFSERSLTNGNRDKVAD
                     AIDLLREKGLIYQGRLEKPKGHDADDWEDREQTLFRSTSFGDDVDRALIKSDGGYTYF
                     ASDIAYHFDKLERGFPRLINVFGADHSGYLKRMHATVDALSGGKADFDIKVCQLVRVF
                     RNGEPVKMSKRAGTFVALRDVVDEVGRDPVRFMMLYRKAEAALDFDLAKVIEQSKDNP
                     VFYVQYAHARIHSVLRNAKEAFPDLDADGAEIAGADLSGLTDAAELDLIKLIAGYPKL
                     IEGAARAAEPHRLAFYLYDLASAFHSLWNRGLDSPHLRLIHSHDRSSTAARIAMIQAT
                     RHVIASGLGILGVQAPSEMR"
     gene            8254..>8550
                     /locus_tag="PK264_17290"
     CDS             8254..>8550
                     /locus_tag="PK264_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77662.1"
                     /translation="MGQPGAPYDLASYAGPAARPGLPASPRDAAPIDPGWGNGQLPAE
                     HPADPGHDGSVEHYPDEAEEPERQSRGKRTIVLVGALIGAIVAGGALAVAYKRFV"
     assembly_gap    8551..8581
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8582..>8836
                     /locus_tag="PK264_17295"
     CDS             <8582..>8836
                     /locus_tag="PK264_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HRD77663.1"
                     /translation="VVAALKAPIKTKPADPGGSEVPHQDNKFLNKLDDAALPAPTPAA
                     PAAASSDGAPRKVATIAIGPDRTAVATPPPGPPPTIPAVPG"
BASE COUNT         1440 a         3004 c         2897 g         1446 t
ORIGIN      
        1 ccggttgagc aggaaggcca acggcagcat gatgaggaga cagaggatgc ccatcacgac
       61 gtgcgtctcg cgccagccga tcgtctcgac catgcgctgg atgagcggcg gccagatcgt
      121 gccggaaatg tagttgccgc tcgcaaccat cgcgacggcg aggccgcgcc ggcgctcgaa
      181 ccacatcgag atgtcggcca tcagcggccc gaacaccgcg gcactgccga agaagccgat
      241 catcacgccc tggacgatgg cgaactgcac gatgctcgtc acctggctga tcaggatgaa
      301 gcccgcgaac agcgagacgc agccgatcag gatcgggatc atgatgccga cgcggtcggc
      361 gagccgcccc atcacatagc cgccgacggc atagccgatc atcgacatcg cgtaggggaa
      421 cgaggcgccg ttcctcgcga tgccgaacgc gtcctgcacg gtcggcagca cgacgaccac
      481 cgaccacatc ccgatattgc cgatcgcgcc gagcaggagg cacacgaaga ggcggcccca
      541 ggaatagagc gaatcctgca cctcggcgtg agcatcgacc gcgccggaac ctatgccgcg
      601 ctgcatggcg tgccttatcg aaagaggggt cgtctcaacg ctcgggcagg atcttgcccg
      661 gattgaggag gccgtccgga tcgagcgcgg ccttgatgcg ccgcatcacc tcgacgccga
      721 caccatgctc cgcttcgaga tacttgatct tgccctggcc aacgccgtgc tcgccggtgc
      781 aggtgccatc catgcgcatg gcacgctcgg tgagtcgctg cacgaaaccc ttggtggctt
      841 ccacctctcg cgggttcgac atgtcgatca tcggctgggt gtggaagttc ccgtcgccga
      901 catggccgag gatcgaggcg atgagcccgg ttttctcgat gtcggccttg gtctcggtga
      961 tgcactcggc gaggcgcgag atcggcacgc acacgtccgt tgccagcgca tcgcggccgg
     1021 ggtaggcgct cttgatcgcc cagtagcctt cgtgccgcgc cttccagagg cggcggcggt
     1081 cctcctcgcg ctccgcccag tcgaagcgga tggcgccctc ggccttggcg atctcctcga
     1141 agcgggcgat ctggtcgcgc gccgaaacct ccgtgccgtg gaactcgagg aagagagtgg
     1201 gtcgctcctc gagaccgagc tttgcgtgct tgttgatcgc cccgatcagg atctcgtcga
     1261 ggagctcgga gcgggcgtgg ccgaggccga gctggattgc ctggatcacc gagttgaccg
     1321 cgccctcgag cgtctcgaag gggcagacgg cggcaacgat cgattccggg atgccgtaga
     1381 ggcggaccga cacctcggtg aagacgccga gcgtgccttc ggagccgacg aggaggcgcg
     1441 tcaggtcata gccggccgac gattttcggg cgcgccgcga ggtgcgcacg atcgagccat
     1501 cggccatgac ggccgtgaga ccgaggacgt tgtcgcgcat ggtgccgtag cggacggctg
     1561 tggtgcctga tgcacgcgtt gcagccatgc cgccaatggt cgcttcctcg gcgccgggat
     1621 cgacggggaa gaaaaggccg agatcgcgca ggtggtcgtt caattgcttg cgcgagacgc
     1681 ccgcctgcac cgtcgcgtcg agatcgagtt ggttgacggc gagcaccgcg ttcatctgct
     1741 ggaaatcgag cgagatgccg ccatgcggcg cgttcacgtg gccctcgagc gaggtgccgg
     1801 cgccgaacgg gattatcggc acgcggtgcg tcgcggcaat ccgcacgatc tcggcgacct
     1861 cctctgtcga gtgcggccag accactgcat cgggaggctc gctcgcgatc caggtcagcg
     1921 tatgggaatg ctggtggcgg atcgcctcgc ccgtctgcac gcggtcgggg aagcgctgcc
     1981 gaagaacggc gattgcggtc gcgacatcgc cttgcgggcc gccggtcgag agcttcgctg
     2041 cgcctgccat gctgtccgtc ctgcctcgcg ctgatatgtg aggcaagcta taggggcaag
     2101 ggggggccca cgcaaccaag ccagtgcagc cctgtgctgt cggttgcgca gatggtgccg
     2161 tgggccctac catgcactct ggtgtcacaa cccgccgtca cgtcaggccc gcgcaggcgg
     2221 cctcatccgg ctcctcggag ggctccgcct ttcaagcccc aagtcgatag acggcaccct
     2281 tcggggcacg ctacaggggt tcctttgagg tttgcggacg ggaccatggc tgaagcggag
     2341 ctgatcggcg tcgattgggg gacgacctcg tttcgcgcct acctgatcgg cgcggacggc
     2401 gccgtcatgg cgcgtcgcga gagcaaagcc ggcattctct cggtcacgaa tgccgcgttc
     2461 cgggagacgc tcgtggccga gctcggcgac tgggcacgct tgcacggcgc gcgtcccatc
     2521 gtcatgtcgg gaatgatcgg cagccggcaa ggctggcgcg aggcgcccta cgtgccgtgc
     2581 ccggcgggac tccgagagat cgctgcggcc ctgatcgaga tcgacggcgg gccgctcggg
     2641 cgcatcgcca tcgtgccagg cctctccatc gaggcggacg gcatccccga cgtgatgcgc
     2701 ggcgaggaga cccagatcgt gggcacactg gtcacggtgc cgtccgagcc gcgctcgatc
     2761 gtcctgcccg gcacgcattc gaagtgggtc gaggtgcgcg acggcaggat cactgccttc
     2821 gagacctaca tgaccggcga gatgttctcg gcggcgcgag atcacacaat cctcggccgc
     2881 cttatgggca cgaccggcgc cgatcgcgag gcggcattcg agaggggtgt cgaggccggc
     2941 gcgcgctcag gcgcacccgg ctcgcttctg catcgactgt tctcggcgcg tacgctcgcg
     3001 ctcttcgatc gcctgccggc cgacgatgtt gcgtcatatc tctcggggct gctgatcggc
     3061 gccgagctcg ccgatcgggg accgcaggcg aagccgatcc tgatcgtggc gagcgagagc
     3121 ctcgctgaat actatcggcg cgccgcggca gtgatcggac tgcaggccga gctcgcgccg
     3181 cccgattgcg tggcggcggg gcatcgtgcg attgcccgcc aggccggact gatcggagat
     3241 ccatcgcgat gaacctcgcc gacgccttgc ggcaatgccc gctgatcgcc atcctgcgcg
     3301 gcattcggcc ggacgaggcc gagccggtcg gcgacgtgct cgtcgaggcc ggattccgcg
     3361 tcatcgaggt gccattcaac tcgcccgagc cgctgcgctc gatcgagcgg ctggcgcggc
     3421 gtctggacaa gcgcgctgtc atcggtgccg gcactgtgat gtcgatcgac gacgtcgaga
     3481 aagttgctgc agcgggcgga cgcctgatcg tcatgccgca caccgatctc gatgtcgtcg
     3541 aggcggcgcg ggatgccggc ctcgccgtga cgccgggtgt ggcaacgcca accgaagcca
     3601 ttgcggcgat tgctgcgggt gtcgacggcc tgaagctctt cccggctgag atgatgctgc
     3661 cgcccgttgt taaggcactg cgcgcggtct tgccgaagga ggtgatcctc attcccgtcg
     3721 gcggcatctc ggcggcaaac attcccgctt accgcgcggc cggcgccgac gctttcggca
     3781 ttggctcgac actttacagc ccgggtaagg ccctcgccga catccgtgcg agtgcagtgg
     3841 cgctggtcaa ggcgtgccgg gtatgacctg cctcagaccg agaagctggt gccgcagccg
     3901 caggaggctg tcgcgttcgg gttcttaatc ttgaaccccg cgccgatgag gtcgtccacg
     3961 aaatcaatct cggatccgag aagatagggc agcgagacgg gatcgacgac gaccttggcg
     4021 ccgccgttgt cgacgatggt atcatccgga tcgctggcgc cgaccagatc gaagcgatac
     4081 tggaagcccg agcaaccgcc gccgtcgacg ctcacgcgca ggatcgcggg tgccgcctcg
     4141 gcacggccga tgtcgcagat gcgggctgca gcccggcttg tcacggtgat gctcatggcg
     4201 aggcccggat gttgaacggc cactgtcatc cggaagatag gtggacggcg cctcttgtca
     4261 aatggcagcc gatgacaaaa gcctgtcgcg gcacagccct gctgcgccgc ggccgctttc
     4321 ggagggctcg atttgagcag acgtgacgag cgagacgcca tgctcagaca acgcggccgt
     4381 gccgtcgccg cccatgcaac cgaggcggcc atgagccgcg ggcggccgat atccgagccg
     4441 gcttnnnnnn nnnnnnnnnn nnccagcgcg accgggaccg catcatccac gcgaccgcct
     4501 tccgccggct cacctacaag acgcaggtgt tcgtcttcca cgagggagat cacttccgaa
     4561 cacggctcac gcacacgctc gaggtcgctc agatcgcccg ggcgatcgcc cgccagctcg
     4621 agatcgacga ggatctgacc gaggcgctgg cactcgccca cgatctcggc catcccccat
     4681 tcggccatgc cggcgagcgg gcgctcgacc gcatgctggc agggcacggt ggtttcgatc
     4741 acaacgcgca gagcctcgcg atcgtcactc ggctcgagcg gaagtatccg gccttcgacg
     4801 gcctgaatct ctcgatcgag acgctcgaag ggctcgtcaa gcacaatggg ccgatcgcgg
     4861 cagcgctcat caccccggac cgggcgctcg ggcgcgtgct ggcatactcg ggtgtcgctg
     4921 atcgcctgtc gctcgatctc tttgcccctc tggaagccca ggccgccgcg atcgccgacg
     4981 acatcgccta caacaatcac gatgtcgacg acggcctgcg cgccggcctc ttcacgctcg
     5041 acgaactcac ctcggtcggc ctggtcgggc cgctggtcga ggcggcggag tgcaaggcga
     5101 tcgaccgggc gcgccgcatc tacgaggtca accgacagct catcacgctg atgatcgccg
     5161 atgtcgtcgc cgaggcgcgg agccggatct cggcgcacgg gtgcgcgagc gtcgacgatg
     5221 tacgccgtgc cggtcggccg atcgtcgagt tctccgatgg gctcaaggtc ggactggccg
     5281 atctcaagcg cttcctgttc acccgcgtct atcgccaccc gcgcgtcatg cgcgtgatgg
     5341 cgagcgcgga gcggatcgtc gaggacctct tccgccgcta tatggccgtt ccgggcgaca
     5401 tccctgagcc gcgccgtgcc gaagcgctcg ccctcaccga ggacggccgc gcgctccacg
     5461 ttgccgactt cgtggccggc atgacggatc gctttgccat cggcgagcac cggcgcctgt
     5521 ttgacgctac tcccgaattg gtctagccgg acccaggttt cagcacgtct ccaaccggcg
     5581 cctccggcgc gaggcaaaca tgaacgtcta ttcgcatctc gagcgcgcga tcctgtcggc
     5641 cgtggacggt atgaaggcgc gtggcgagct tcccaacgaa ctcgttgcgt ccgccggcga
     5701 cattgaccct ccgcgcgatc catcccacgg cgaccttgcg agcaatgtgg caatggtgct
     5761 cgccaaggcc gcgaagaaga agccacgcga catcgccgag gcgatagcca ggcagcttgc
     5821 ggccgacccg ctgattgcca aggccgacgt cgcgggacca ggctttctca atatcaagtt
     5881 gaagcccgcg gcctggcacg gcgtcgtcca gacggcggtc aaggcaggtc cggactacgg
     5941 ccgatccgac ctcggccggg gcagcgcggt caatgtcgag tacgtgtcgg ccaacccgac
     6001 cggcccaatg cacgtcggcc attgccgtgg cgccgtctat ggcgacgtgc tcgcaagcct
     6061 gctcgccttc gccggctaca aggtgacgcg cgagtactac atcaacgatg ccggcgctca
     6121 ggtcggcaag ctcgcccagt cgaccctgtt gcgttaccgc gaggcgctcg gcgaagcgat
     6181 cggtgagatc cccgaaggcc tgtaccccgg cgactatctg aagcccgtcg gcgcggcgct
     6241 cgcggcaagg ttcggccgga agctcctaga gaagcctctc cccgagcagc tggccgaggc
     6301 gcgcgctgcc gccgtctcga ccatgatggc cgagatcaag gatgatctcg cggcgctcgc
     6361 cgtccgtcac gatgtcttct tttccgagcg ctcgctgacc aatggcaatc gcgacaaggt
     6421 cgccgacgcc atcgacctgt tgcgcgagaa gggcctcatc taccaaggcc ggctcgagaa
     6481 gcccaagggg cacgatgccg acgattggga ggaccgcgag cagacgctct tccgctcgac
     6541 ctcattcggc gacgatgtcg accgcgcttt gatcaagtcg gatggcggct acacctattt
     6601 tgcctccgac atcgcctatc acttcgacaa gctcgagcgc ggctttcccc ggctgatcaa
     6661 cgtttttggg gcggatcact ccggctacct gaagcgcatg catgcaacgg tcgatgcgct
     6721 gtcgggcggc aaggccgact tcgacatcaa agtctgccag ctcgtccgcg tgttccgcaa
     6781 cggcgagccc gtgaagatgt cgaagcgcgc cggcacgttc gttgcgctcc gcgatgtcgt
     6841 cgacgaggtc gggcgcgatc ccgtgcgctt catgatgctc taccgcaagg ccgaggcggc
     6901 gctcgacttc gatctcgcca aggtcatcga acagtcgaag gacaaccccg tcttctatgt
     6961 gcagtacgct cacgcgcgca tccattcggt gctgcgcaat gccaaggagg cattccccga
     7021 cctcgacgcc gatggcgccg agattgcagg ggccgatttg tcgggcctca ccgatgcggc
     7081 cgagctcgac ctcatcaagc tgatcgcggg ctatccgaag ctcatcgagg gcgccgcccg
     7141 tgccgccgag ccccatcggc tcgccttcta tctttacgat ctggcctcgg ccttccattc
     7201 gctctggaac cgaggccttg attcgccaca tttgcggctt atccacagcc atgatcggag
     7261 ttcgactgcc gcacggatcg caatgatcca ggccacgcga cacgtcatcg cttccggttt
     7321 ggggattctg ggggtgcaag caccgagcga gatgcggtaa tctacgattc tcgtaggttt
     7381 ggactactga agcttgagca gcgcgacgat gatgcgatca ctggagtagg gcggcgacct
     7441 cgcggcgtct gatcgttggc tggtcgggac cgggactcga gctgcgacga atgaactgac
     7501 aaagttgaag gagaccggca gatgtctcgg tcgaatggcc cattgccgcc ccatgcgggc
     7561 catcccgccc gcccggaagc ccacgatccg gaagcggcat cacggcttgc ccagttggca
     7621 gcattgcggc gcggtgccgc tgctggacat gtcctaccgc acgccccgcc ctatgggcag
     7681 ccgcccgccc agccccagtc ggcacatccg ggaagccccg tgccggcgcc cgcgtatcac
     7741 ggccagccac gtcaacccac cgatcccgcc gcattcgggc atgccgcgcc ccagcccgca
     7801 taccggcatt ccgcgcaacc ccagcccaac ggccatgccg caccgcatcc gcggacccag
     7861 ccgccggctc cctcgcacga tccttttgct gctccgcctg cgggccatga cccgcgctac
     7921 ggcgccttcg cggctcctca gacggcgcgt ggggcaccac agccaaggcc ggcctacgcg
     7981 ccggcattcg atccctatgc gcagccgtcc ggccacgacc cgcgcctttc ggcgcctgcg
     8041 ggcatcgggc tggcccccgg acatcccgcg tcggcaggtc cggcatacgg tgcgccggcc
     8101 tttggtgctc catctcagcc gccaggcgcg ccgtcctacg tgcagccgca gcgcgcgacg
     8161 ctcggacagc cgcagccttc gttcgatccg cgcgatcctc gactcgctgc ccctcctccc
     8221 cgcgcgcccg gtcaagatcc gttcgcgctg cctatggggc agccgggggc gccctacgat
     8281 ctcgccagct atgcgggtcc agcggcacga cccggcctgc cggcctcgcc gcgagatgcg
     8341 gcgccgattg atcccggctg gggtaatggt cagttgccag ccgaacatcc cgccgatccg
     8401 ggccatgatg gctcggtcga gcattatcca gacgaggccg aggagccgga gcgtcagagc
     8461 cgcggcaagc ggactatcgt gctggtcggc gcgctcattg gcgccattgt ggccggcggc
     8521 gcacttgccg tcgcctacaa gcgcttcgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nggtggtcgc cgccttaaag gcgccgatca agaccaagcc ggccgatccc ggcggcagcg
     8641 aggtgccaca tcaggacaat aagttcctga acaagctcga cgatgctgcg ttgccggcgc
     8701 cgacgccagc ggcgcccgcg gctgcatcct cggacggcgc gccgaggaaa gtcgcgacga
     8761 tcgccatcgg accggatcgg accgcggttg ccacgcctcc acctggtccg ccgccgacca
     8821 tccccgccgt gccggg
//
LOCUS       DAORNH010000194         8815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5897_length_8815_cov_2.775913, whole genome shotgun sequence.
ACCESSION   DAORNH010000194 DAORNH010000000
VERSION     DAORNH010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_8815_cov_2.775913"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PK264_17300"
     CDS             <1..902
                     /locus_tag="PK264_17300"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD77664.1"
                     /translation="LGARPVHWTRIAGEAVEARAWPEPGTHSGALVRIPKEKEALAFA
                     LGGAAARVPAGAVIAVAGQNAEGARSAGRQLERFANRIETVDTRHHCRIIAGRRRADL
                     AGAGRELADWRRRAPLEVDPPVPDWTSYPGLFAGGRLDDGTRLLISALPALGGEPRVL
                     DFACGTGVIAHAIRRLMPDAHIEAIDHDALAVLAAGENVAGLAARTGRGLSETDLRDL
                     DLIVSNPPLHDGIREDLTVLRTLVAEAPERLTPTGQVLLVIQRRIRPEAFVSGGRIEV
                     TSICATSRFEVHSIRFARASGKR"
     assembly_gap    1166..1203
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1204..3951
                     /locus_tag="PK264_17305"
     CDS             <1204..3951
                     /locus_tag="PK264_17305"
                     /inference="COORDINATES: protein motif:HMM:NF019158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfB"
                     /protein_id="HRD77665.1"
                     /translation="AGRSSAQDGSAGERAGPRAPADAVVTLIPNSGLQFIDLAVPSTA
                     YAAVVLRFLEEPDPADASVRVLHGLAIGAIYHPILRTPIAPQDTHRITGREALEAFRG
                     AWIPLPFLRILKTRRQGGLALDEGPTNWVRGFLDRSADGEYLLTLAIDTLLDTEAREP
                     GDAYPAPTNEDAHFQSSFVFDHESDQLAAVLSEPWLIAWIEGQLERHAVGRLPGLGEA
                     TFTHGALAYYMTFLSVLAGAAVLPRLRFAGATARGRQTNVVPVDLVLDVGASRTSGIL
                     VEALGAKATDDISSATLLSLRDLGRPTVTHAGSFSSHTEFARPSFGSEALSRRSGRTN
                     AFLWPSLVRIGAEAERIANQSGAADGHTGLASPKRHLTETEATPYVWRFAANGDEPGG
                     MVAGPLLASLTEGGDVLARSPRTRVPALRPRFSRSSLLCFFMTEVLLQAISHINSPSR
                     RSERRQADAPRQLRRIVLTTPLTMSTDARELLRLRVEGAIDLVWQAFGWGVDGASVEA
                     ADGERLLPPRPEVRLGFDESMCTQLVYIYDAITQRFQGAPADYFNLMGKPRVEHGLHP
                     TLRIAGLDIGAQSSTLTIVTYAASNDGAITPTLTIAEACRIGGDAIVEAVVARFILPT
                     IERALARAGLARPRDFLAETFGTSARGAYAPADEQTQLRQRFVKRFAVPVAQALMSRY
                     ESLGTDQDTDQYSVRLGDLMGHAHGVNASVVTAFDARAKQAGAVGFLLGDIELEILRS
                     DVSTAVREVLRPMIATVTPVVRSFDCDLLLLSGWSTRLGDVMAMLLDQLPLRPDRIVP
                     IHEYRVGSWYPFRSPSRRVDDPKSAVVMGALLGSLDIGRISTPLDRVHRVKATHFLDD
                     IPPVDTRYDAPIDLDTAEDHPLAARPDETFSPAGAAGEARRAAAVVLEPRRDRSGARA
                     S"
     gene            3948..6560
                     /locus_tag="PK264_17310"
     CDS             3948..6560
                     /locus_tag="PK264_17310"
                     /inference="COORDINATES: protein motif:HMM:NF021642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfC family protein"
                     /protein_id="HRD77666.1"
                     /translation="MSFLERRLAIECRQSGEALAKIIDWLEARSDELDAENGPLCRSL
                     RRSLKRIDVLAAAALQPASIGFVGTAGSGKSSLIAAVATGAPAGIARLSVALDQTIPP
                     VDALTMLAGGTAVPGTRADRSSGVGLAVRYLVKSTAGPPGFPLTFELMSQLDVVKVLA
                     RAYFSNCPLKSDLVPEAEQVRVMALEAGQHMSLFQMRGLGEDDIAALRDDIERRYHDE
                     PLVKALTASGYWEYCRAIISHLPEGARAALLSVLWGGSPTLTTIYLKAAGALETLAHA
                     SEIHCPLEALVAPEGMAGPSAPHPASIAFTETLHRGDHREAPLGVRTKLGQFASIERS
                     VLALLVAELRLTLAEPRSLAADLAALVEMPTPAPRPFGVPSGARLDQQTAIALFAREK
                     SAYLLERATDRSDLNALVVCLDPSGRDPDTLSAAVGDWVEAMHGDEPAEREASETALF
                     IAATKVNLSVPVTAAADGRSSVLDGIGRMLVATLGRSHRWPLEWTSGQAFDNVFLIRH
                     ARAGDDGAAPAGQRVRNALVARASAGLPALTTGMREVAAHPIAERHFQDIVKAVAEAE
                     DPADGGISYLSDSIAEVLQSGLRQRQLAQSLRRLKLHLRDVLLRKYPGADPQRQRLWR
                     LRDQRVALARLKQAARSQRFGHLMRELQVSEVELAEVFQHIETSTRDGKPGSEATKAE
                     KSPGGLVRLYAAAAVGYWLDHVRLLSEAKALQRRIGIPGPVLTQIVEEMAAGARAMGL
                     VGQLATRLSWMSSDRMTVEERVARSAVHAAHVIAGFVATLGYDDQRAEGHPRRVGRNR
                     EPIFSGRHNGEAGSPLSAYARVGEGGDDWQVAYAGMVRSSVAGQGGRGDAEHERQISE
                     LLSVLGVTGLEYEP"
     gene            6557..7993
                     /locus_tag="PK264_17315"
     CDS             6557..7993
                     /locus_tag="PK264_17315"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD77667.1"
                     /translation="MSKVWLEVAEDKSRPEGGHAIIRLHGVAGLTTKPSFSIHPLDGD
                     GPSQRLVTDQYEPISSRETSEGTELVVGPDVVDNPALLPGTPVAIEVPSVGVRAEVLW
                     PSITPLARQRKQPVIMTTARRLSLVQASRDQTKKVAPRIEVAEGIHALAGGPAPLAGR
                     LDVVVPLPMALSASAAAAPLSMEPQRHLEAEPVLAALAVPLSSDGGRPARPAADHRGE
                     AGPAPLPEPAPASASTARPSVSDSVVAKLVEASNPPTPVATAPYYPIQRYEPPQTDKV
                     GRRLSGIAMMMALLAIGSSLLVLQLVRSMRAGPQMAMMTPVIGNGDAALPVPPSTLFD
                     IFSVGTKSPSGKNAASIDAAKALAMADTLIHSAGSPSDLKEGEFWIKRALTLSLADER
                     SRWALTQLGSLYAAPTEGDPNYERARLLWEMSAALGDPVAMCFLGSLHQHGLGVPASR
                     GNALKWFELARQTGGCKGLDDLVAKLKR"
     gene            8382..>8578
                     /locus_tag="PK264_17320"
     CDS             8382..>8578
                     /locus_tag="PK264_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77668.1"
                     /translation="MKADEEKRVAAEAESKRKSEEAARAAAAEEARKAAADAEAARRR
                     AEEERRVAAEAEAKRKSEEAAR"
     assembly_gap    8579..8596
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1360 a         2935 c         3052 g         1412 t
ORIGIN      
        1 ggctgggcgc gaggcccgtg cattggaccc ggatcgccgg cgaggccgtc gaagcgcgag
       61 cctggcccga gcccggcacg cactccggcg ccttggttcg catccccaag gagaaggagg
      121 cgctcgcctt cgcgctgggc ggtgccgcgg cgcgcgtacc ggccggtgcg gtgatcgcgg
      181 tcgcgggcca gaacgccgag ggcgcacgct cggccggtcg ccagctcgag cggttcgcca
      241 accgcatcga gaccgtggat acgcgtcacc actgccggat cattgccggc cggcgcaggg
      301 ccgatctcgc tggagcgggc cgcgagctcg ccgactggcg acggagggca cctctcgagg
      361 tcgacccgcc ggtgcccgac tggacctcct atccgggcct cttcgctggc ggccggctag
      421 acgacggcac gcgactgctg atctcggcgc tgccggcact cggcggcgag cctcgcgtcc
      481 tcgatttcgc ttgcggcacg ggtgtcatcg ctcacgccat ccggcggcta atgccggacg
      541 cgcacatcga ggcaatcgat cacgatgccc tggccgtcct ggctgccgga gagaatgttg
      601 cggggctcgc ggcgcggacc ggccgagggc tctccgagac tgatctcaga gacctagacc
      661 tcatcgtctc caacccgccg ctccacgatg gcatccgcga ggacctgacg gtgcttcgga
      721 cgctcgtcgc cgaggcgcca gaacggctca ccccgaccgg gcaagtgctg ctcgtcatcc
      781 agcggcgcat acgccccgag gcttttgtct ccggcgggcg gatcgaggtg acctcaattt
      841 gcgcgacaag ccgattcgag gtgcactcga tccggttcgc gcgcgcgtcc ggcaagcgtt
      901 agtgatttct gcggccgtga attgcgaagc gcgttcacgg accgtactat tcgtgccgac
      961 cagcagcggc ctccggtcga gtgtgtccat cctgacgcgc aacctacgac ttttgtcgcg
     1021 agcacggctc agcacgctat tccagccgtc aggaaagtgc gataaagacg cgggggagtg
     1081 tgcgtattga gtagaatgac aatgcatggc tcggggggag taaaaggggc acgagaccct
     1141 gtcacgcgcg cgtccgcgga ggggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnngccggcc ggagttctgc acaggatggt tctgccgggg agcgagcggg gccacgcgcg
     1261 ccggccgacg ccgtcgtcac tctcattccg aatagcggcc ttcaattcat tgatctggct
     1321 gtcccttcga cggcgtatgc tgcagtcgtc ctgcgcttcc tcgaggagcc ggatcccgcc
     1381 gacgcgtccg tccgcgtcct gcacgggctc gcgatcgggg cgatctatca tccgatcctc
     1441 aggacaccga tcgcgccgca ggatacgcac cgcatcaccg gccgcgaggc gctcgaggct
     1501 ttcagggggg cctggatacc gctccctttc ttgcgcattc tgaagacccg ccggcagggc
     1561 ggcctggctc tcgacgaagg cccgaccaac tgggttcgcg gtttcctcga ccgcagtgct
     1621 gacggcgagt atcttctcac gcttgccatc gacactctgc tcgataccga ggcccgcgag
     1681 ccgggtgacg cctatcccgc gccgacgaac gaggacgccc atttccagag cagcttcgtc
     1741 ttcgaccatg agagcgacca gctggccgcg gtgctgtcgg agccgtggct catcgcgtgg
     1801 atcgagggtc aactcgagcg ccatgcggtc ggccgtctgc ctgggctcgg cgaagccaca
     1861 ttcacgcacg gggcgctcgc ctactacatg acgtttttgt ccgtactcgc gggagccgct
     1921 gtcctgccga ggctgcggtt cgcgggcgcg acggcacgtg gacggcagac caacgtggtt
     1981 ccggtcgatc tcgtgctcga cgtcggcgcg agccgcacgt ccggcatcct ggtcgaggca
     2041 ctgggcgcca aggcgacgga cgacatctcc tccgcgacgt tgctgagcct gcgcgatctc
     2101 ggccgcccga ccgtcacgca tgccgggtcc ttctcgagcc ataccgagtt cgcccggccg
     2161 tcgttcggca gcgaagcgtt gtcgcggcgg agcgggcgca ccaacgcctt cctctggccg
     2221 agcctcgtca ggatcggcgc cgaggcggag cggatcgcca accagagcgg ggcggcggac
     2281 ggccatacgg ggctcgcgag cccgaagcgc catctgaccg agaccgaggc aacaccatat
     2341 gtctggcggt tcgccgcaaa cggggacgag cccggcggca tggtggcggg accgctgctc
     2401 gcgagcctca cggaaggcgg cgatgtgctg gcgcggtcgc cgaggacgcg cgttcccgcg
     2461 cttcgcccgc gcttctcgcg ctcatcgctg ctctgcttct tcatgactga ggtgctgctg
     2521 caggccatct cgcatatcaa ctctccgagc cggcggtcag agcgtcggca ggccgatgcg
     2581 ccgcgccagc tccgccggat cgtgctgacg acgccgctca ccatgtccac ggatgcccgc
     2641 gagttgctgc gcttgcgcgt cgagggggcc atcgatctcg tgtggcaggc cttcggctgg
     2701 ggcgtcgacg gcgcctcagt cgaggcggcg gacggcgagc ggctcctgcc gccgcgtccc
     2761 gaggtgcggc tcgggttcga tgaatcgatg tgcacgcagc tcgtctatat ctacgacgcc
     2821 atcacgcagc gcttccaagg tgcgccggcc gactacttca acctgatggg caagccgcgg
     2881 gtcgagcacg ggctgcatcc gacgctgagg attgccggcc tcgacatcgg tgcgcagtcg
     2941 tcgacgctca cgatcgtcac ctatgcggcc tcgaacgacg gagcgatcac gccgacgctc
     3001 accatcgcgg aggcctgtcg gatcggaggc gatgccatcg tcgaggcggt cgtcgcgcgc
     3061 ttcatcctgc cgacgatcga gcgcgcgctc gcgcgtgcgg ggcttgcgcg accgcgcgac
     3121 ttcctggccg agacattcgg gacatccgcg cgcggggcct atgcgcccgc tgacgagcag
     3181 acacagcttc gccagcgctt cgtgaagcgg ttcgcggtgc cggtcgccca ggcgctaatg
     3241 tcgcgctacg agagcctcgg aacggaccag gacacggacc agtattcagt gcgcctcggc
     3301 gatctcatgg gacacgcgca cggcgtcaat gcctctgtcg tcacggcgtt cgatgccagg
     3361 gccaagcagg cgggggcggt cggcttcttg ctgggtgaca tcgagctcga gatcttgcgc
     3421 agcgacgtct ccaccgcggt ccgcgaggtg ctgcggccga tgatcgccac cgtgacgccg
     3481 gtggtgcgct cgttcgactg cgatctgctg ctgctgtccg gctggtcgac tcgcctcggc
     3541 gatgtgatgg ccatgctgct cgaccagctg ccgctccggc ccgaccggat cgtgccgatc
     3601 cacgagtacc gggtgggcag ctggtatccg ttccgctcgc cgtcacgacg ggtcgacgat
     3661 ccgaagtccg ccgtggtcat gggtgcgctg ctcggcagcc tggatatcgg ccgaatttca
     3721 actccgctcg accgggtgca tcgcgtgaag gctacgcatt ttctcgacga cattccaccg
     3781 gtcgatacgc gctatgatgc gccgatcgat ctcgatacgg ccgaggacca tccgctcgca
     3841 gcccgtcccg acgagacgtt ctcgcccgcc ggtgccgcag gcgaggcccg ccgggccgcg
     3901 gcggtcgtgt tggagcctcg gcgtgatcgc agcggcgcga gggcttcatg agctttctgg
     3961 agcgccggct tgcaatcgag tgccggcagt cgggcgaagc gcttgccaag atcattgact
     4021 ggctcgaggc gcgctccgac gaactcgatg ccgagaacgg ccccctctgc cgcagcttga
     4081 gacgctcgct gaagcggatc gatgtcctgg cggcggccgc attgcagccg gcgtcgatcg
     4141 ggttcgtggg taccgcgggc tccggcaagt cctcgctcat cgcggcggtc gctaccggtg
     4201 cgcccgccgg catcgcacgc ctctccgtgg cgctcgatca gacgataccg ccggtcgatg
     4261 cactgacgat gctcgccggt ggcacggcgg ttccgggcac ccgagccgac cgcagctcag
     4321 gcgtcggcct cgccgtgcgc tatctggtca aatcgacggc cgggccgccg ggcttccccc
     4381 tcacattcga gctcatgagc cagctcgacg tcgtcaaggt gctcgcccgc gcctatttct
     4441 cgaattgccc gctgaaatcc gatctcgtgc ctgaggccga gcaggtgcgc gtcatggcgc
     4501 ttgaggccgg ccagcacatg agcctctttc agatgcgggg gctcggcgag gacgacatag
     4561 cggcgctccg cgacgacatc gagcgccgat atcatgacga gccgctggtc aaggctctca
     4621 cggcctcggg ctactgggag tattgccgcg caatcatatc tcatctcccc gagggggcgc
     4681 gggccgcact cctctcggtg ctctggggcg gcagcccgac gctgacgacg atctatctca
     4741 aggccgccgg cgcccttgaa accctcgccc acgcgagcga gatacactgc cctctcgagg
     4801 cactggttgc gccggaaggg atggcaggcc cgagtgcgcc gcatccggcc agtatcgcct
     4861 tcacggagac gctccatcgc ggcgaccacc gcgaggcccc gctcggggtg cgcaccaagc
     4921 tcggccagtt cgccagcatc gagcggagcg tgctggcact gctcgttgcc gagctgcggc
     4981 tgacgctcgc cgagccccgg tctctcgcgg cagatctcgc cgcgctcgtc gagatgccga
     5041 cgccggcgcc gcgtcccttt ggcgttccgt cgggggctcg ccttgatcag cagacggcca
     5101 ttgctctctt cgcacgcgag aagtccgcct atctcctcga gcgggcgacg gaccgctccg
     5161 acctcaacgc cctggttgtc tgcctcgacc cttcgggacg cgatcccgac acgctctcgg
     5221 cggctgtcgg cgattgggtc gaggccatgc atggggacga gccggccgag cgcgaggcgt
     5281 cggaaacggc gctcttcatc gctgcgacca aggtaaacct ctcggtgccg gtgactgccg
     5341 cagcggatgg acgctcgagc gtcctcgacg gcatcgggcg catgctggtc gcgacgctcg
     5401 ggcgctcgca tcgctggccg ctggaatgga cgtcgggaca ggccttcgac aacgtcttcc
     5461 tcattcgtca tgcgcgcgca ggcgatgatg gggcggcgcc tgccgggcag cgggtgcgca
     5521 atgctctggt tgcgcgcgcg agtgccgggc tacccgcgct gacgacgggc atgcgtgagg
     5581 tcgctgccca tccgatcgcc gagcggcact tccaggacat cgtcaaagcc gtggccgaag
     5641 ccgaggatcc ggctgacggc ggcatcagct atctctcgga ttcgattgcc gaggtgctcc
     5701 agtcgggcct ccgccaacgc cagctcgcgc aaagcctgcg gcggctgaag ctgcatttgc
     5761 gtgacgtgct gctgcgcaaa tatccgggtg ccgatccgca gcgccagcga ttgtggcggc
     5821 tcagggatca gcgcgtggcg ctggccaggc tgaagcaggc ggcgcgctcg cagcgcttcg
     5881 gccacctcat gcgcgagctc caggtctccg aggtggagct cgcggaggtc ttccagcaca
     5941 tcgagacctc gacgcgcgac ggcaaaccgg gctccgaggc gacaaaggcc gaaaaatcac
     6001 cgggtggtct ggtgcgcctc tatgctgccg cggcggtcgg ctattggctc gatcatgtga
     6061 ggctgctctc ggaagccaag gcattgcagc gacgtatcgg catccccggg cctgtgctga
     6121 cccagatcgt cgaagagatg gccgcggggg cccgcgccat ggggcttgtg ggacagctgg
     6181 caactcgcct aagctggatg tcgagcgacc ggatgacggt cgaggagcgt gtcgccaggt
     6241 cggctgtgca cgcagcgcac gtcatcgcgg gtttcgtcgc gacgcttggc tacgacgacc
     6301 agcgcgccga agggcatccg cggcgcgtgg gccggaatcg ggagccgata ttcagtggcc
     6361 ggcataacgg ggaggcgggt tccccgttgt cggcctatgc tagggtcggc gaaggcggcg
     6421 atgactggca ggtcgcctac gcaggcatgg tcaggagcag cgtcgcaggt cagggcggcc
     6481 gcggagatgc ggagcacgag cgccagatca gcgaattgct gtccgtattg ggtgtcacag
     6541 ggttggagta cgagccatga gcaaggtttg gcttgaagtc gccgaggaca agtcccgccc
     6601 tgaaggcggc cacgccatca tccgcctgca tggcgtggcg ggcctcacaa cgaagccgag
     6661 cttttcgatc cacccgctcg acggagacgg accgtcgcag cgtctcgtga ccgatcagta
     6721 cgagcccata tcgtcccgcg agacgagcga ggggaccgaa ctggtcgtcg gtcctgacgt
     6781 tgtcgacaac ccggcgctgc tgccagggac gcccgtggcg atcgaggtgc cctccgtcgg
     6841 tgttcgtgcc gaggttctct ggccgagcat cacaccgctc gcgcggcagc gcaagcagcc
     6901 ggtcatcatg accaccgcgc gcaggctgtc tctcgtgcag gcatcccgcg atcagacgaa
     6961 gaaggtggcg ccgcggatcg aggtcgccga gggaattcac gccctcgcag gcgggccggc
     7021 gcctctggcg ggacggctcg atgtcgtcgt accgttgccg atggcattgt cggcgagcgc
     7081 ggcagccgca cccctgtcga tggagccaca gcgtcacttg gaagccgagc cggtgctcgc
     7141 ggcactcgcc gttccgctct cctctgacgg aggtcgcccg gcgcggcctg ccgcagatca
     7201 tcgaggagag gcggggcccg cgccgctccc cgagcctgct cccgcgtcag cgagcacggc
     7261 gcggccgtcc gtgtccgatt ccgtcgtcgc caagctcgtc gaggcgtcca atccaccgac
     7321 gccggtggcg acggcgccct actatccgat ccagcgctac gagccgccgc agaccgacaa
     7381 ggtcggccga aggctctccg gtatcgccat gatgatggcg ctcctcgcca tcggctcctc
     7441 actgctggtg ctgcaactcg tgcgcagcat gcgggcgggc ccgcagatgg ctatgatgac
     7501 gcctgtcatc ggcaatggcg acgccgcatt gccggtgccg ccatcgacgt tgttcgatat
     7561 tttcagcgtt ggcacgaaat cgccaagcgg caagaatgcc gccagcatcg atgcggccaa
     7621 ggcgctggcc atggccgata cgctcatcca ttctgccggt tcgccgagcg atctcaagga
     7681 gggcgagttc tggatcaagc gcgcgcttac gctctcgctc gctgatgagc ggagccgatg
     7741 ggctctcacg cagctcgggt cgctctatgc cgcgccgacg gaaggggatc ccaactacga
     7801 gcgggcgcgc ctgctctggg agatgtcggc ggcgctcggt gatccggtgg cgatgtgctt
     7861 cctcggcagc ctgcaccagc acggcctcgg cgtgccggcg agtcggggca atgcgctcaa
     7921 gtggttcgag ctcgcgcgcc agacgggagg ctgcaagggg ctcgacgatc tggtggccaa
     7981 gttgaagcga tgacgtgtgc acgggccgcc ggtcgggtcc gtgttgagga tcgcgatgta
     8041 gaagatcgcg gtgtcgaggt tcgcgatggc gcggtgccgg ggcagcaacg catcccgggc
     8101 cgcctcattt cagaggtcaa gacatgacgg gtcggtcacg ggcgcggttt tggatgtcgg
     8161 gcgcggcagc gctgccgctc gcgcttacga ttgcatgggc cggtgcaacc tcggcgcaac
     8221 agcctccggc ggcgggattg ccgagcctga aggaccagcg caggctgccc gggacggttg
     8281 gtgccactcc ggatccggcc gtcgacaagc acggagcaga tgaagctctg cgcaaagcag
     8341 aggaagcgcg caaggccgct gccgacgctg aggccgctcg attgaaggcc gacgaggaaa
     8401 agcgcgtcgc ggcagaagcc gagtcgaagc gcaaatccga agaggcggct cgcgctgctg
     8461 cggcggagga agcgcgcaag gccgctgccg acgcagaggc tgcccgccgg agggccgaag
     8521 aggaaaggcg cgtcgcggcc gaggccgagg cgaagcgcaa gtctgaggaa gcagctcgnn
     8581 nnnnnnnnnn nnnnnngcgg aagtccgctg ccgacgcaga ggctgcccgc cggaaggccg
     8641 acgaggaaaa gcgcgtcgcg gcggaatcag aggagaagcg caaggccgag gaagcggctc
     8701 gcgttgctgc ggccgaggcc gcccggaagg ccgccgccga cgcagaagct gcccgcaaga
     8761 aggctgacga ggagaagcgc gtcgcggctg aagccgaagc tcgccggaag gccga
//
LOCUS       DAORNH010000195         8732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_5971_length_8732_cov_1.779302, whole genome shotgun sequence.
ACCESSION   DAORNH010000195 DAORNH010000000
VERSION     DAORNH010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5971_length_8732_cov_1.779302"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>246
                     /locus_tag="PK264_17325"
     CDS             <1..>246
                     /locus_tag="PK264_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRD77669.1"
                     /translation="SARWAGPTKDFSIAMRKVEDALKGKRDRRAKFVAALALAWPDGR
                     CDVFEGEVDGALVWPPRGAKGFGYDPMFVPEGYAITF"
     assembly_gap    247..399
                     /estimated_length=153
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <400..876
                     /locus_tag="PK264_17330"
     CDS             <400..876
                     /locus_tag="PK264_17330"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HRD77670.1"
                     /translation="YCDFNSHVRERVEEARWQRAYLAELERAAAETPGRVVTSVFFGG
                     GTPSLMPPETAAAILAIARQLKENGAAAKFGTYHLAGSGTTTWCGLAREALAVAGRHG
                     GRSVPVHAISSAEYPTPARRPKNSVLDCRRSTGAFGVTLPEWQAGVEASVRRLLSP"
     gene            1163..>1687
                     /locus_tag="PK264_17335"
     CDS             1163..>1687
                     /locus_tag="PK264_17335"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77671.1"
                     /translation="MRHPACALALAAAALHSMPTVSSASPPVHECDRLAASPYDREAV
                     VKDRDHRADIGAEAKAACEAAIAAHPGVARFEYQLGRALLHAATSDAEFASAIAMLKR
                     AAARGHTMAMTEIGEHYMTHGSIPDYAEAARWLTKALEGGDDGAAHNLGVLYFGGQGV
                     DEDHGVALGLFRRAA"
     assembly_gap    1688..1699
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1700..2302
                     /locus_tag="PK264_17340"
     CDS             <1700..2302
                     /locus_tag="PK264_17340"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77672.1"
                     /translation="IHVAATMIERGDGTAADVAEAVRWYERAAEKGTTDAMFRLGEMS
                     LDGIGMGRDDGAAFKWFERGAKAGHPRSKAMLGYLYETGRGTTASLMRAASLYAEAAA
                     AYDARGKLLNAFVRMPMGALSGQAEALAEEIYQAASLTSIRGEKEWMLKYVQNNFTRI
                     PPVVVMALHARLKRGKQLVDLPGAPIEEVVLKSIERLIML"
     gene            2448..3227
                     /locus_tag="PK264_17345"
     CDS             2448..3227
                     /locus_tag="PK264_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD77673.1"
                     /translation="MDLGIKGRKAIVCAASKGLGRGCAEALAREGVEVTICARTAAEL
                     EATAAAIRSSTGGKVTAVACDVTTEDGRKKLLAACPAPDILINNAGGPPPGDFKDFSL
                     EDWRKAVEGNMITPIALIKATVYGMMDRKFGRIINITSQSVKAPIASLELSNGARAGL
                     TGAVAVIARKAARHNVTINALLPGPFDTDRLKGSTKKMAEMKGISPEEMAKHRKAEVP
                     AGRFGHPDEFGAAAAFLASTHAGFITGQNLLMDGGIYPGTM"
     gene            complement(3253..>3694)
                     /locus_tag="PK264_17350"
     CDS             complement(3253..>3694)
                     /locus_tag="PK264_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD77674.1"
                     /translation="GGRALVDGGLVNPLPFDLVAAECDVTIAIDVSAAALEPEPGSHP
                     SAFEVVISSTQILQRSIVREKLRATRPDVYIDVEVDQFSILAFHSWADILATAAPAKD
                     RLKRQLERVLASQTAEAIDASAAPAAVAAPEEPKRRRFLPPRKR"
     assembly_gap    3695..3730
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3731..4170)
                     /locus_tag="PK264_17355"
     CDS             complement(<3731..4170)
                     /locus_tag="PK264_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRD77675.1"
                     /translation="MPHVDCHRGARGLAHILVLEALEEMGIRPSLIAGTSIGAIYGAA
                     AAAGLPAKLIRAHTEEILGNRFDFVRQLIGARAAPVQRLLSFIPLRSSLLDPQAVLEL
                     VLPSRMPARFDELATPLKVVATDLYAQTPAVLSEGALKPAVAAS"
     assembly_gap    4437..4592
                     /estimated_length=156
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4593..4830)
                     /locus_tag="PK264_17360"
     CDS             complement(<4593..4830)
                     /locus_tag="PK264_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77676.1"
                     /translation="MLAAVEPEIPVADRGVYEDAMMGLARARMGIRPEMAAADAHALQ
                     RNRLHLLQGPDELDLHEQQLRLLSRGKSGERASDL"
     gene            5239..5661
                     /locus_tag="PK264_17365"
     CDS             5239..5661
                     /locus_tag="PK264_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77677.1"
                     /translation="MALAATFHPDFDDASKRAKSATNRDRWHAHWVILNEDIACPGGL
                     KVKDIPAGTTPKLPPDWPGAPILIDSPEYKTELSGDIVEVHVPLAEIGALANASFDGV
                     TAGLKVNANLHAPLLCVINVFKVASGNLSLPGRVAPAR"
     gene            5750..6295
                     /locus_tag="PK264_17370"
     CDS             5750..6295
                     /locus_tag="PK264_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563687.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRD77678.1"
                     /translation="MSHVNLVDPASANAAVKPTFDQIKTAFGVVPNMFKTVANSPAAL
                     QSMWGSFGALGDGSIGAKLGEQIAVAVADRNNCNYCLAAHTVLGQKAGASATEMVEAQ
                     SGRSADPKTAAALAFVIKVVENRAAVSASDVQALRTVGFDDGAIVEILAHVALNLFTN
                     YLNVALDVPVDFPAVKLSRAS"
     gene            6361..6906
                     /locus_tag="PK264_17375"
     CDS             6361..6906
                     /locus_tag="PK264_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRD77679.1"
                     /translation="MTATAPELSVTRWLNAPRPLSLAALRGKIVVLHAFQMLCPGCVA
                     HALPQAERLHRLVKNRSDVAVIGLHTVFEHHAAMSPVSLEAFVHEYRLTFPIGVDQPA
                     DGEAIPVTMRRFGMRGTPTTIVIGPAGSIVEHAFGQVDDLALGMLIGSLAPRNLIASA
                     APAYEEGCGDGFCPAPASEAV"
     gene            6906..7586
                     /locus_tag="PK264_17380"
     CDS             6906..7586
                     /locus_tag="PK264_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HRD77680.1"
                     /translation="MLPLSPTVLVGAVAALGRGGPPSGIGKWPVAGPWQIARLGLAGD
                     AQGDLKHHGGPEKAIHQYPFDHYAAWEAEIGPHPLLAAPGAFGENLSGKGWTEETICI
                     GDVARFGRSLLQVSQGRQPCFKLNLRFGRTDMARDLQRTGRTGWYWRVLEEGEVQQGA
                     ALAVVDRPRADWPLSRLLKLLYHDLDNRADIAAMAELPELAENWRVLARRRIKSGRVE
                     DWSNRLNS"
     gene            complement(7641..>8220)
                     /locus_tag="PK264_17385"
     CDS             complement(7641..>8220)
                     /locus_tag="PK264_17385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD77681.1"
                     /translation="RRGQLDVGVVAEASPGRGYAVTNLWNESVYLAMPESDPLADQKA
                     VRWRDLRDRHFVVTDLPTGDFARAYLERHLEVPPDALRIEQLSVTPESLMQIVALGGG
                     VTIAGSAHVRLGLSGITFRLIEDAMLRYGAVHSNGSLQRDLERLLALAKSLSERDQAW
                     FVRQRLIRPEHRRGLQVDCHRGKRGRIPDPMQ"
     assembly_gap    8221..8250
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8251..8638)
                     /locus_tag="PK264_17390"
     CDS             complement(<8251..8638)
                     /locus_tag="PK264_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD77682.1"
                     /translation="MPRPKIELRHLRYIIAAAESGSFRRAAQDLGVEQSAISRSIRDV
                     EDEIGAQLFRRHSAGVELTEIGKQFLHQASPGARQIGSALERAMTTAATGRHLRVGIF
                     GPLTMGFLSELFGTFRRERPGTRLRFS"
BASE COUNT         1479 a         2790 c         2677 g         1399 t
ORIGIN      
        1 attcggcgcg ctgggccggg ccgacgaagg atttttcgat cgcgatgcgc aaggtcgagg
       61 acgcattgaa gggcaagcgg gatcgccgcg cgaaattcgt ggcggcgctg gcgctcgcct
      121 ggccggacgg gcgctgcgac gtgttcgagg gcgaagtgga cggcgcgctg gtgtggccgc
      181 cgcgcggcgc gaaaggcttc ggctacgatc cgatgttcgt gcccgaaggg tacgcgatca
      241 cgttcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt attgcgattt caattcgcat
      421 gtccgcgagc gcgtcgagga agcgcgctgg cagcgcgcct atctcgccga gctcgagcgc
      481 gccgcggcgg aaacgcccgg ccgcgtcgtc acctcggtct tcttcggcgg cggcacgccc
      541 tcgctgatgc cgcccgaaac cgcggccgcg atcctcgcca ttgcccggca gctgaaggaa
      601 aacggcgcgg cggcaaagtt tggcacctac cacctcgccg gcagcggtac gacgacgtgg
      661 tgcggccttg cgcgcgaggc gctcgccgtc gccgggcggc acggcgggcg atcagttccc
      721 gtgcatgcga tctcgagtgc cgaatatccg acgccggcgc ggaggccgaa aaattcggtc
      781 ctcgattgcc gtcgctcgac gggggcgttc ggggtgacgc tgccagagtg gcaggcgggt
      841 gtcgaggcga gtgtgcggcg cttgctcagc ccctgagccg cgtcgtgctg gacgcgcatc
      901 cggggagctc ccaattcctg agtatcgaac gtggccaggt ccggggcggg ccgagccccg
      961 gtcgggtccg ggcccgaggc ccggtcgcgc agagaaaatc ggtggactat ccgtgggcgc
     1021 tccgatgcgg ctcagcgcca atacgccagt tgccccgccg gacttcagct cacgcaccag
     1081 atgggttttc gcgcctcgtc gcacagtgct acgctacgcg ccagtcattg cccgatctcg
     1141 atgcccgttc cggagctatg cgatgcgaca tccagcgtgc gcgcttgcct tggctgctgc
     1201 cgcactccac tcgatgccca cggtctcgtc ggcttccccg cctgtgcacg agtgcgaccg
     1261 cctcgccgcg agcccctatg atcgcgaggc cgtcgtcaag gatcgcgacc accgcgccga
     1321 cattggtgcg gaggcgaagg cggcctgcga agccgcgatt gccgcgcatc cgggcgtcgc
     1381 ccgcttcgag taccaactcg gccgcgcctt gctgcatgcc gcaacttctg atgccgagtt
     1441 cgcgtcggcc atcgcgatgc tgaagcgcgc cgctgcgcgt ggccacacga tggccatgac
     1501 cgagatcggc gagcactaca tgacgcacgg ctcgatacca gactacgcgg aagccgcgcg
     1561 ctggctgacg aaggcgctgg agggcggcga tgacggcgcc gcgcacaatc tcggcgtgct
     1621 ctatttcggc ggacagggcg tcgacgagga ccacggcgtc gcgctcggcc tgtttcggcg
     1681 cgccgccnnn nnnnnnnnna ttcatgtcgc agccaccatg atcgagcgtg gcgatggcac
     1741 ggccgccgat gtcgccgagg ctgtccgctg gtacgagcgg gcggccgaga agggcacgac
     1801 cgacgccatg ttccgcctcg gcgagatgag cctcgacggc atcggcatgg gccgggacga
     1861 tggcgccgcg ttcaagtggt tcgagcgcgg cgcgaaagcg ggacaccctc gcagcaaagc
     1921 gatgctcggc tatctctacg agacgggccg gggcacgacg gcgagcctga tgcgcgcggc
     1981 tagcctttac gctgaagcgg ccgctgccta cgacgcccgc ggcaagctcc tcaatgcatt
     2041 cgttcgtatg ccgatggggg cgctgtcggg acaggcggag gctctcgccg aggagatcta
     2101 ccaggctgcc tctctgacat caattagggg tgaaaaggaa tggatgctca agtacgtcca
     2161 gaacaatttc acccggatcc caccagtcgt cgtcatggcg ttgcatgccc gattgaagcg
     2221 cggcaagcag ctcgtcgatc tgccgggtgc gccgatcgag gaagtcgtgc tgaaatcgat
     2281 cgagcgactg atcatgttgt agtgagctcc gggcatcaga cgccgatctc tgccacggca
     2341 cccgcacgag agcgaaatcc cttgaacgcc agcgctgcgg gccgcatgct tccgactgaa
     2401 gcgtccggcc gaggccgtgt cgcccaaatc aagaccggag gaaccccatg gatctcggga
     2461 tcaagggccg caaggccatc gtctgcgcgg cgtcgaaggg cctcggccgc ggctgtgccg
     2521 aagcgctggc ccgcgaaggc gtggaggtga cgatctgcgc ccgcaccgcc gccgagctcg
     2581 aggcgacggc tgccgccatc cggtcctcga ctggcggcaa ggtgacggct gtcgcctgcg
     2641 acgtgacgac cgaggacggc cgcaagaagc tgctcgccgc ctgccccgcg cccgacatcc
     2701 tcatcaacaa tgccggcggc ccgccacccg gtgacttcaa ggacttctcg ctcgaggatt
     2761 ggcgcaaggc cgtcgaaggg aacatgatca cgccgatcgc cctcatcaag gcgaccgtct
     2821 acggcatgat ggaccgcaag ttcggccgca tcatcaacat caccagccag tccgtgaagg
     2881 caccgatcgc ctcgctcgag ctttcgaacg gcgcgcgcgc gggcctcacc ggcgcggttg
     2941 ccgtgatcgc gcgtaaggcg gcccggcaca acgtcaccat aaatgcgctg ctgcccggcc
     3001 ccttcgacac cgatcgtcta aagggctcga ccaagaagat ggccgaaatg aagggcattt
     3061 cacccgagga gatggcgaag catcgcaagg ccgaggtgcc ggccggccgc ttcggccacc
     3121 cggacgagtt tggtgcggcg gcggcattcc ttgcgtccac gcacgcgggc ttcatcaccg
     3181 ggcagaacct gctcatggac ggcggcatct atccgggcac gatgtgagcg ggccccgcgg
     3241 agcgctgatg gctcagcgct ttcgcggtgg caggaagcgg cgacgcttcg gctcctcagg
     3301 cgccgcgacc gccgccgggg cggcgctcgc gtcgatcgcc tcggcagtct ggctcgcgag
     3361 cactcgctca agctggcgct tcaggcggtc cttggccggc gctgctgtcg caaggatgtc
     3421 ggcccacgag tggaacgcga ggatgctgaa ctgatcgacc tcgacgtcga tgtagacatc
     3481 agggcgcgtc gcacggagct tctcgcgcac gatcgagcgc tgcaggatct gcgtggacga
     3541 gatcacgacc tcgaaggccg acgggtgcga gccgggctcc ggctcgagag ctgccgcgct
     3601 gacgtcgatg gcgatggtga cgtcgcactc ggcggcgacg aggtcgaacg gcagcgggtt
     3661 gacgaggccc ccgtcgacga gagcacggcc gccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn atgctggcgg cgactgcggg tttgagcgcg ccttccgaca gcaccgcggg
     3781 cgtctgcgca tagagatcgg tcgcgaccac cttgagcggg gtcgcaagct cgtcgaagcg
     3841 tgccggcatg cgcgagggca gcacgagctc gagcacggcc tgcggatcga gcagcgagga
     3901 acgcagaggg atgaagctca agagccgctg cacgggtgcg gcacgggcgc cgatgagctg
     3961 ccgcacgaaa tcgaagcggt tgcccagaat ctcctcggta tgcgcccgga tgagctttgc
     4021 cggcagaccg gcagccgccg ccgcgccgta gatcgcgccg atggacgtgc ctgcgatgag
     4081 cgacggcctg atgcccatct cttcgagtgc ttcgaggaca agaatgtgcg cgagcccgcg
     4141 cgcgccgcga tggcagtcaa cgtgcggcac gcgccaatgc tcagggcgca tgaggcgtga
     4201 acgcttagaa tccaaacgcc tcttgactat attacttagg agtcctattc ttaaattcat
     4261 ggatcacgca aacactcccg attctgttcc gacccgcctg cacgagggat tcgagcgcct
     4321 cgccctggtc ctgcgtgctg acctttggag cgcttccggc gcagagggcc tcaatccgac
     4381 gcaggctcaa gtgctggcct tggtggcggg gcggtccgat ggcttgcgcg ccaaagnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaggtca gaagcgcgct cgccgctctt
     4621 gccccggctg agcaggcgca actgctgctc gtgcagatca agctcatccg gaccttgcag
     4681 cagatgaagg cgattccgct gcagcgcatg tgcgtcagct gccgccattt ccggccgaat
     4741 gcccatccgg gcgcgagcca gccccatcat tgcatcttcg taaacgccgc gatcggcgac
     4801 cgggatctcc ggctcgactg cggcgagcat gaagcggccg atccggctgt ccagtctgcc
     4861 aactggatgg cgttcgacgc ggagcggtcg tccctccaag cccaatcatg acctgagaag
     4921 gacacactcc caatgagatt ctacaacctg cggggcctcg gcctcacggc gacggctctg
     4981 ctttgcctca gtgccagcgt ctcggcgcat gagatcaagg ccgcctccaa ccaaacgcta
     5041 accgcatcct tcgatatcat tgagactagg atcaccacag atcacgggca cgccatattc
     5101 cgcactcgcg tccgcgcggc cgcaggagcc gcgatcccac aggcgaccgg caaattcgaa
     5161 ggctccgagg tctacgccta tgtctggcca acaagcctga actcggccga tgtcggcttc
     5221 gacatggatc agggcatagt ggcgctcgcc gcgaccttcc atccggattt cgacgatgcc
     5281 tcgaagagag cgaaaagcgc gaccaaccgc gaccgatggc atgcgcactg ggttatcctt
     5341 aacgaggaca ttgcctgccc cggcggcctc aaggttaagg acatcccggc gggaaccacg
     5401 cccaagctgc cgccagactg gcccggcgcg ccgatcctga ttgatagccc tgaatacaag
     5461 acagaactct cgggcgacat agtcgaagtc catgtgcctc ttgccgagat cggcgctctg
     5521 gccaacgcga gtttcgacgg cgtgacggcg ggtctcaagg tcaacgccaa cctgcacgcg
     5581 ccgcttcttt gcgtcatcaa tgtcttcaag gtcgcctcgg gcaatctgag cctgcccggt
     5641 cgtgtggctc ctgcgcgctg aggcgcgccg aaacgaaagg cggcccgacg actgccatcg
     5701 ccggaccgcc cgatcatccc cctccaagca tcagagaagg aaaagtctta tgtcacatgt
     5761 gaacctcgtc gatccagcct ccgcaaacgc agcggtgaag cccacgttcg accagatcaa
     5821 aaccgccttc ggcgtcgtcc cgaacatgtt caagacggtc gcgaactcgc ctgccgcgct
     5881 ccagagcatg tggggttcgt tcggcgcgct cggcgacggc tcgatcggcg ccaagctcgg
     5941 cgagcagatc gccgtcgcag tcgccgaccg caacaactgc aactactgct tggctgctca
     6001 cacggtactc ggccaaaagg ccggcgcaag cgcgactgag atggtcgagg cgcaatccgg
     6061 ccgctcggcc gaccccaaga ctgcggcagc gctcgccttt gtgatcaagg tggtcgagaa
     6121 ccgtgcagcg gtgtcagcgt ccgacgtgca ggccctgcgc acagtcggat tcgacgacgg
     6181 cgcgatcgtt gagatcctag cgcatgtcgc gctcaatctg ttcaccaact atctgaacgt
     6241 cgccctcgac gtgccggtgg actttcccgc agtgaagctc agccgcgcct cgtgagccgg
     6301 caggcggtcg cgggatcttc ccgcgaccgc cccttcgaag gtctcgatcc ggagtccgac
     6361 atgaccgcta ccgcgcccga actctccgtc acgcgttggt taaacgcccc gcgtccgctc
     6421 tcactcgccg cactgcgcgg caagatcgta gtcctgcacg ccttccagat gctctgtccc
     6481 ggctgcgtcg cacacgcctt gccgcaggcg gagcgcttgc accgccttgt caagaaccgc
     6541 tccgacgtcg ccgtgatcgg gctccacacc gtgtttgaac accacgccgc gatgagtcca
     6601 gtctcgctcg aagccttcgt ccacgaatat cggctgacct tccccatcgg cgtcgatcag
     6661 ccggccgacg gcgaggccat cccggtcacg atgcggcgct tcggcatgcg gggtacgcca
     6721 acgacgatcg tcatcggccc agcaggcagt atcgtcgagc atgccttcgg gcaggttgat
     6781 gacctcgcgt tgggtatgct gattggatcg cttgcgccgc gaaatctcat agcaagtgcc
     6841 gcacccgcgt atgaagaagg atgcggggac ggcttctgcc ccgcgccagc cagcgaagcc
     6901 gtctgatgct tccgctctcg cccaccgttc ttgtcggcgc agtcgctgct ttaggccggg
     6961 gcggccctcc aagcggcatt ggcaagtggc cagtcgctgg accctggcag attgcgcgcc
     7021 tgggattggc cggcgacgcg cagggcgatc tcaagcatca cggaggcccg gaaaaggcga
     7081 tccaccagta tcctttcgac cactatgcag catgggaggc cgagatcggt ccccacccgc
     7141 tgctggccgc acccggagcc ttcggcgaga atctgtcagg caagggctgg accgaggaga
     7201 ctatctgcat cggcgacgtg gcgcgcttcg gcagatccct tcttcaggtc agccaagggc
     7261 gccaaccctg cttcaagcta aacctgcgct tcgggcgcac ggacatggcg cgcgacttgc
     7321 aacgcacagg gcgcaccggt tggtattggc gcgtgctgga ggaaggagaa gtccagcagg
     7381 gcgccgcact tgcggttgtt gaccgcccgc gtgccgactg gccgctaagt cgtcttctca
     7441 aactactata ccacgatctt gacaatcgag ccgacattgc tgccatggcg gaactgcccg
     7501 aacttgccga gaactggcgc gtgctcgcgc ggcgacggat caaaagcggt cgggtggaag
     7561 attggtcaaa tcgtctgaac agctgacgac tgcgcaggcc taccccatct ctcgaccagg
     7621 cctgcccttg ctcgagcgct tcattgcatc ggatcgggga ttcgcccgcg cttgccacga
     7681 tggcagtcta cttgcagccc acgccgatgc tcaggacgga tgagtcgctg ccggacgaac
     7741 catgcctgat ctctctccga cagggacttt gccagcgcca gcagacgttc gagatctcgc
     7801 tggagggacc cattggaatg gacggcgccg tagcgaagca tggcgtcctc gatcaggcga
     7861 aaggtgatgc cggaaaggcc gagacggaca tgggcgctgc ccgcgatcgt aacgccgccg
     7921 ccaagcgcga cgatctgcat taatgactcc ggcgtcacgg acagctgctc aatgcgcagc
     7981 gcgtcgggcg ggacctccag atgcctctct agataggccc tcgcgaaatc gcccgtcggc
     8041 aagtcggtca ctacgaagtg ccgatcccgc agatcacgcc atcgaaccgc cttctgatcg
     8101 gccagcgggt cgctctccgg catcgctagg tagacagact cgttccacaa attcgtcact
     8161 gcatagcccc ttcccggcga agcctcggcg accacaccga catctagctg cccgcgtcgc
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgctgaaccg cagacgggtg ccgggccttt
     8281 ctcgccgaaa cgtaccgaag agctccgaca gaaatcccat ggtcagtggc ccgaagatgc
     8341 cgaccctgag atgtcgcccc gtggccgctg tcgtcattgc tctctcgagc gctgaaccga
     8401 tctgacgcgc gcccgggctg gcctgatgca ggaactgttt gccgatctcc gtcagctcaa
     8461 cgccggcact gtggcggcgg aacagttggg ctccgatctc atcttcgaca tcccggatgc
     8521 tccgactgat cgccgactgc tcgacgccaa ggtcctgtgc cgcgcgccgg aagctgccgc
     8581 tctctgcggc ggcgatgata tagcgcagat gacgtagctc tatcttcggt ctcggcacga
     8641 tggttctcca ttgtgcaaag ttctttgcaa acgcagccca gtcagccccc gagaaccttt
     8701 ccaatcagat tactgtcctt ctccgtctcc tc
//
LOCUS       DAORNH010000196         8711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6000_length_8711_cov_2.996303, whole genome shotgun sequence.
ACCESSION   DAORNH010000196 DAORNH010000000
VERSION     DAORNH010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6000_length_8711_cov_2.996303"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PK264_17395"
     CDS             complement(<1..589)
                     /locus_tag="PK264_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77683.1"
                     /translation="MTTVAAPDCESDAPGGATPPSGADRSADLTLPATLEFLPEPRTD
                     TTVGGTRFSIAVVAALGLHALAVLGLVYVGGTDLAGAGGQTLEGISVDVVSLDQIIAI
                     PETRAAASSAPAVASDGAPHAVDPPPPRLEAKTDPPPEHMEPPADVPPGTPDLTAKPE
                     DTPPPDAIILPHALGANICGVITSAIFAAVLVTVVR"
     gene            complement(674..2269)
                     /gene="serA"
                     /locus_tag="PK264_17400"
     CDS             complement(674..2269)
                     /gene="serA"
                     /locus_tag="PK264_17400"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRD77684.1"
                     /translation="MTSTAAKPRVLIADDLSPAAVAIFKERGIAADMKVGLDKAEFER
                     IIADYDGLAVRSATKVTAKVFEAAKRLKVVGRAGIGVDNIDVKAATERGVIVMNTPFG
                     NSITTAEHAISMMMALARQIPSADASTQAGKWEKSRFMGVELFGKTLGIIGCGNIGAI
                     VASRGIGLKMRVIAFDPFLSEDRAREIGVEKVELDDLFRRSHFITLHTPLTEKTRNIV
                     DTRAIALMRPDVRIINCARGGLVDEKALAEAIKAGKVAGAAFDVFEVEPAKSNALFGL
                     ANVVCTPHLGAATLEAQENVALQVADQMSEYLLRGAVSNAINFPSITAEEAPRLKPWV
                     KLAEQLGLFAGQLTETSIRGVRLVYSGLGDMNTKPLSAVAIASVLRPLLADSVNMVSA
                     ASIAKERGMRIEETTRGKEGAFESYMKLTVVTDEYERSVAGTVFSDGRPRIIQVRDIG
                     MEFELTPNMLFVRNADKPGFIGRFGGVLGEAGVNIATMNLGRDKPGGDAICIVAVDGP
                     VSDAVMSKVRELPQVVRVNRLTF"
     gene            2581..3186
                     /locus_tag="PK264_17405"
     CDS             2581..3186
                     /locus_tag="PK264_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD77685.1"
                     /translation="MSDITLEASQEVGTVRKLWPSETDKFRDHLLRLDRGSRRMRFAH
                     AVSDTFIADYATRMLEFGSICYGYIVDGEIRAVAELRKLSETWGEEAEAAFSVEAAYQ
                     DRGIGSELMGRVIRAARNRGVRLLYMSCLAENERMQAIAKKYEADLRFELGDVVGEIV
                     PEEANYFSIMAEAAEDRVSYLMAVFDLQSRVINRLTQRIAG"
     gene            complement(3195..3626)
                     /locus_tag="PK264_17410"
     CDS             complement(3195..3626)
                     /locus_tag="PK264_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77686.1"
                     /translation="MYELSRNWDGITSLTIVVAAMAGLALTFYTGRTLVGRRSGQPMR
                     NGRTRTPRERTPNVTAHREQVAITGSAGQQWDRLDAVLRTAARRTRTASALQHEAWQK
                     IDAARHDIDAVLLDIDPLLARGPGGVTSSEPTAGLGEGAKS"
     gene            complement(3876..4202)
                     /locus_tag="PK264_17415"
     CDS             complement(3876..4202)
                     /locus_tag="PK264_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HRD77687.1"
                     /translation="MSTFDEREKGFEKKFAHDADLKFRAEARRNKMVAEWAGAKLGLS
                     GAAMEDYIKAVRKSDLAEKGNDDVIRKVLKDLTDKGIKSSEAEIGKLMSEFLAKAVAD
                     IEGAKS"
     gene            4574..5362
                     /locus_tag="PK264_17420"
     CDS             4574..5362
                     /locus_tag="PK264_17420"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRD77688.1"
                     /translation="MNRRRRVYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHALID
                     GKGVLNNRISEYIFVKLGEIGVPTHFMKRLNMREQLIREVEIIPLEVVVRNVAAGSLA
                     TRLGLEEGTPLPRSIIEFYYKNDKLNDPMVSEEHITAFGWATPQEIDEVMQLALRIND
                     FLVGLFLGIGIKLVDFKVEFGRLWEQNDQMRIVLADEISPDCCRLWDASSGDKLDKDR
                     FRRDMGGVLEAYQEVARRLGVLTENPRPKGSGPVLVSSNGAKPS"
     gene            complement(5680..6690)
                     /locus_tag="PK264_17425"
     CDS             complement(5680..6690)
                     /locus_tag="PK264_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD77689.1"
                     /translation="MKTVTLGHSGLTVSRLCLGTMMFGGPTPEAEAHRIMDDALAKGV
                     NFLDSANAYTEGRSEEVIGRGLKGKRDRWIVATKVGNPIGPGVADRGLTRRHVMMSCE
                     ASLRRLGIEALDILYIHKLDPGTSWEQTVLTFGDLIRQGKIREWALSNVRAWHIPHIH
                     HLCRDAGVPAPTALQPYYNLVNRQPEVELLPAARHFGLGVVPYSPIARGVLTGKYKLN
                     QTPEAGSRAARKDKRMMETEWRPESLAIAEAVDAHAKARGTTPVVWATAFVLANAAVT
                     SVIAGPRTFEQWTSYQAALDYHWDSDDEALAARLVPPGHASTHGYTDPAYPVEGRVLA
                     KG"
     gene            complement(7203..7622)
                     /gene="fosX"
                     /locus_tag="PK264_17430"
     CDS             complement(7203..7622)
                     /gene="fosX"
                     /locus_tag="PK264_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosX/FosE/FosI family fosfomycin resistance
                     hydrolase"
                     /protein_id="HRD77690.1"
                     /translation="MSRIEGLSHMTFIVRDLDRMEAILVSVLGAEKVYDSGTATFSLS
                     KERFYLAGSLWIAVMEGEPLPTRSYNHIAFKISEADYDDCLGRVRSLGLDIREGRARV
                     AGEGRSIYFHDHDNHMFELHTGTLEERLARYAKARAG"
     gene            complement(7619..>7872)
                     /locus_tag="PK264_17435"
     CDS             complement(7619..>7872)
                     /locus_tag="PK264_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD77691.1"
                     /translation="AAVGDIRGRGLLIGIDLVRDRDSKAPDNDLAEAVLYRALDRGLS
                     FKTTMGSVLTLTPPLTITEDQMMAALGIIEEAIAAGHDR"
     assembly_gap    7873..7911
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7912..8665)
                     /locus_tag="PK264_17440"
     CDS             complement(<7912..8665)
                     /locus_tag="PK264_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD77692.1"
                     /translation="MALAKKLASIAPGGLSKMLFTTGGSDAVEVAVKIARTATGRHKT
                     LSFWDAFHGAGFGAAALSGEALFRSGPAAPLISGDQHVAPFACYRCAYGHDVDASGEP
                     HLERCKLACARMVDYVLSRETDVACVVAEPARAVPYIPPPGFWQSVRAACDRHGALLV
                     FDEIPTGLGKTGRMFASEHDGVAPDILVMGKALGGGILPIAAVVTRPDLDVGADYAFG
                     HYTHEKNPVTCRAALTTIEIIEDERLVENAARV"
BASE COUNT         1418 a         2733 c         2988 g         1533 t
ORIGIN      
        1 ccctgacaac cgtcaccaag acggcagcaa agatcgccga cgtgatgacg ccgcagatgt
       61 tagcacccaa cgcatgcggc aggatgatcg cgtccggcgg cggcgtatcc tcgggctttg
      121 ctgtgaggtc cggcgtcccg ggcggcacgt ctgccggtgg ttccatgtgc tccggtgggg
      181 gatcggtctt cgcctcgagg cgggggggcg gcggatcgac tgcatgcggc gctccgtcgc
      241 tcgcaacggc gggcgcgctg ctcgccgcgg cgcgggtttc ggggatggcg atgatctgat
      301 cgagcgatac gacgtcgacg ctgatcccct cgagggtctg tccgccggcg ccggcgagat
      361 cagtgccccc gacgtagacg aggccaagta cggcgagcgc atgaaggccg agtgctgcca
      421 ctacggcgat cgaaaagcgc gtgccaccga ccgttgtgtc cgtgcgcggt tcggggagga
      481 actcgagcgt cgccggcaac gtcaagtcgg ccgagcgatc ggcaccggag ggtggtgtgg
      541 cacctcccgg cgcatcggac tcgcagtctg gtgccgccac tgtcgtcatt acttgagcta
      601 cgatccagga tatagcgcac gctgcagcgc tcaatcgagc gccgcggcgc tcgaatgtaa
      661 tacagtaaca gctctagaat gtcagccggt tgacgcgcac gacctggggc agctcacgca
      721 ccttgctcat cacggcgtcg gacacgggac cgtcgacggc gacgatgcag atggcgtcgc
      781 cacccggctt atcgcggcca aggttcatgg tggcgatgtt gacgcctgcc tctccgagca
      841 cgccgccaaa gcggccaatg aagcccggct tgtcagcatt gcgcacgaag agcatgtttg
      901 gggtcagctc gaattccatg ccgatgtcgc gcacctggat gatgcgcgga cgcccgtcgg
      961 agaagacggt gccggcgacg gaacgctcgt actcgtccgt gacgacggtg agtttcatgt
     1021 agctctcgaa ggcaccttcc ttgccgcgcg tcgtctcctc gatgcgcatg ccgcgctcct
     1081 tggcgatcga ggccgccgag accatgttga cgctgtcggc gaggagtggg cggaggacgc
     1141 tggcgatggc gacggcggag agcggcttcg tgttcatgtc gccgaggccc gagtagacga
     1201 ggcgcacgcc tcggatactc gtctcggtga gctggcccgc aaagaggccg agctgttcgg
     1261 cgagcttcac ccacggcttt aggcgcggcg cctcctcggc cgtgatcgag gggaagttga
     1321 tcgcattcga gacggcgccg cgcagcaggt actccgacat ctggtcggcc acctggaggg
     1381 ccacgttctc ctgcgcctcg agggttgctg cgccgaggtg cggcgtgcac acgacattgg
     1441 cgaggccaaa gagcgcgttg gatttcgcgg gctcgacctc aaagacgtcg aaggcggccc
     1501 cggcaacctt gccggccttg atggcctcgg cgagcgcttt ctcgtcgaca agcccgccgc
     1561 gggcgcaatt gatgatgcgc acgtcggggc gcatcagtgc gatggcgcgc gtatcgacga
     1621 tgttgcgcgt cttctccgtg aggggggtgt gcagcgtgat gaagtggctg cgcctgaaga
     1681 ggtcgtcgag ctcgaccttc tcgacgccga tctcgcgggc gcgatcctcc gagaggaacg
     1741 gatcgaaggc gatcacacgc atcttgaggc cgatcccccg cgaggcgacg atggcgccga
     1801 tgttgccgca tccgatgatg ccgagcgtct tgccgaagag ttcgacgccc atgaagcgcg
     1861 atttctccca cttgccggct tgcgtggagg cgtcggccga ggggatctgg cgggcgagcg
     1921 ccatcatcat ggagatggcg tgctcggcgg tggtgatgga gttgccgaag ggtgtgttca
     1981 tgacgatcac gccgcgctcg gtcgcagcct tgacgtcgat gttgtcgacg ccgatgccgg
     2041 ccctgccgac gaccttgagg cgcttggctg cctcgaagac cttggccgtg accttggtcg
     2101 cggagcgcac cgcaagccca tcgtagtcgg cgatgatgcg ctcgaattcg gccttgtcga
     2161 gcccgacctt catgtcggcg gcgatgccgc gctccttgaa gatggcgacg gccgctggcg
     2221 agaggtcgtc ggcgatgagg acgcggggct tggcggcggt cgaggtcatg gtctcgcctt
     2281 tctgatacga ctattggcgg gcatctatgc cgcactgcag cactgctatg caattggcgt
     2341 tttgctgcag gggccgaagg tatgacccga ggcgggcaag cgaccgtgag cttgctgcgc
     2401 gaggagccgc acgagcgcct gtcgcactgc agcgtatcga cgcacatcac tcgaatgtgt
     2461 ggagtatttg cacgcatttt catcgttctg ccatctgagc tgcccattgt gcaatgcgaa
     2521 gaagctgcat aggttcgtgg tgtccgacac atcattcgga cgcagaagac gagggccgtc
     2581 atgagcgaca tcacactcga ggcttcgcag gaggtcggca cagtccggaa gttgtggcca
     2641 tctgaaacgg acaagttccg cgaccatctc cttcgcctcg accggggctc gcggcgcatg
     2701 cggttcgcgc acgccgtctc cgacaccttc atcgccgact acgcgacgcg catgctcgag
     2761 ttcggctcga tctgctacgg ctacatcgtc gatggcgaga tccgggctgt cgctgaactc
     2821 cgcaagcttt ccgagacctg gggcgaggag gccgaggcgg ccttctcggt ggaggccgcc
     2881 taccaggacc gcggcatcgg gagcgagttg atgggacgcg tcattcgcgc ggcgcggaat
     2941 cggggcgtcc ggctccttta catgagctgc ctcgccgaga acgagcgcat gcaggcgatc
     3001 gccaagaagt acgaggcgga tctgcgattc gagctcggcg acgtggtcgg cgagatcgtg
     3061 cctgaggagg ccaactactt ctcgatcatg gccgaggccg cggaggaccg cgtgagctat
     3121 ctcatggccg tgttcgatct ccagtcacgc gtcatcaacc ggctgacgca gcggatcgcg
     3181 gggtagcacg atcgtcagga cttggcgcct tcgccgaggc ctgcggtggg ctctgacgac
     3241 gtgaccccgc ccgggccgcg cgcgaggagt gggtcgatgt cgaggagcac ggcgtcgatg
     3301 tcgtggcggg cggcgtcgat cttctgccag gcctcgtgct ggagtgcgga ggcggtccgc
     3361 gtgcgacggg cggccgtgcg tagaaccgcg tcgaggcggt cccactgctg gccggcgctc
     3421 ccggtgattg cgacttgctc gcggtgtgcc gtcacgttgg gggtacgctc gcgtggagtg
     3481 cgcgttctgc cgtttcgcat cggctgcccg ctgcgccggc cgacgagcgt ccggcctgtg
     3541 tagaacgtga gcgcgaggcc ggccatcgct gccacgacga tggtgagcga ggtgatgccg
     3601 tcccaattcc gggagagctc gtacaaggtt gtgctgatcg acgacacgcg gacgaccgag
     3661 gctcgagtgc ggcaggcacg cgtcgcgctg gcccgatcgc tgcggccgcc tccagccgca
     3721 gtcctggccg agccggccac ggccgtaaag tccgtgctcg aatgttgctt aatccgcggt
     3781 cggcacagtt ctcgcacgac gccggcatga ataaggccgc gctgctagcg gccgcgcgac
     3841 acaggtctcg gtctttgccg gatggctggt ggcgctcagc tcttggcgcc ctcgatgtcg
     3901 gcgacggcct tggcgagaaa ctcgctcatg agcttgccga tctcggcctc gctcgacttg
     3961 atacccttgt cggtcaggtc cttcaagacc ttgcggatca cgtcgtcatt gcccttctcg
     4021 gcaaggtcgg acttgcggac ggccttaatg tagtcctcca tcgcggcgcc agagagcccg
     4081 agcttggcgc cggcccattc ggcgaccatc ttgttgcggc gggcctcggc acggaacttc
     4141 aagtcggcat cgtgcgcgaa cttcttctcg aagccctttt cgcgctcatc gaaggttgac
     4201 atgtgctacc cctggttgcg atcgacgctg tttttgtcgc agctgcaact gacagtcgtg
     4261 tccgatcgaa ggcctttccg caccagttag cgatcggcag gccggcttgc aacggggtgt
     4321 gcacacctgc cgggaatatt tcgctcctta gccatcgcgg atggacggcg gcctgttaag
     4381 attgtacgcc atggccggtt cggataatct ccggcgatcg atccgggacg tcggcgaacg
     4441 ggcatgctgc acgtgcgaaa tgctcgctgc ggcggccgct cgtctccgag gggtcggccc
     4501 gccacatgcc tcgcgacggc gtttgtcgcg acggcaagta cctcgagttg atgtgccacg
     4561 aaggattcaa gcgatgaaca ggcggcgccg cgtgtacgaa ggcaaggcca aggtccttta
     4621 cgaggggccg gagcccggca cgctcattca gcatttcaag gacgacgcga cggcgttcaa
     4681 cgccaagaag cacgcgctca tcgacggcaa gggcgtgctc aacaaccgca tctcggaata
     4741 catcttcgtg aagctcggcg agatcggcgt gccgacgcac ttcatgaagc gtctcaacat
     4801 gcgcgagcag ctgatccgcg aggtcgagat catcccgctt gaggttgtcg tgcgcaatgt
     4861 cgcggccggc agtctcgcga cgcgcctcgg cctcgaggaa gggacgccgc tcccccgctc
     4921 gataatcgag ttctactaca agaacgacaa gctcaacgac ccgatggtct cggaggagca
     4981 catcaccgcg ttcggctggg cgacgccgca ggagatcgac gaggtgatgc agctcgcgct
     5041 gcgcatcaac gacttcctcg tcggcctgtt cctcggcatc ggcatcaagc tcgtcgactt
     5101 caaggtcgag ttcggccgcc tctgggagca gaacgaccag atgcgcatcg ttctcgccga
     5161 cgagatcagc ccggactgct gccggctctg ggatgcctcg agcggcgaca agctcgacaa
     5221 ggaccgcttc cgccgcgaca tgggcggcgt gctcgaggcc taccaggagg tcgcgcggcg
     5281 gctcggcgtg ctgaccgaga acccgcgacc gaagggttcc ggacccgtgc tcgtctcgtc
     5341 gaacggtgcc aagccgagct gatgaggacg gcccgactga cgtcgccgcc ggacccgatc
     5401 gcgtccagcg tccgctaccg tcgtctatcg accgctgggc gcgacaacca aagagggtgc
     5461 gagcgacgcg cgcaattcgc cgcgcgacgg ggcctctcac tgagtttgag cgccatccgc
     5521 gcgcgagccc caacccgatc gggactcggg caggctcgaa gccggccctt ttcagggctc
     5581 atcatctctg cgtcagggcc gccccgtgtc agggtacgag gctagctccg gcgcccctcg
     5641 cggttcgtca cggcaagttc gccagactcc cgggctgcat cagcccttgg cgagcacgcg
     5701 cccttcgacc ggataggccg gatcggtgta gccgtgcgtc gaggcgtggc ccggcggcac
     5761 cagcctggcg gcaagcgcct cgtcatcgct gtcccagtgg tagtcgagcg ccgcctgata
     5821 gctcgtccac tgctcgaagg tgcggggtcc ggcgatcacg gaggtgacgg ccgcattggc
     5881 gagcacgaag gccgtggccc acactaccgg cgtggtgcct cgcgctttcg cgtgggcgtc
     5941 gacggcctcg gcgatggcga ggctttcggg gcgccattcg gtctccatca tgcgcttgtc
     6001 cttgcgcgca gctcgcgagc ccgcctcggg cgtctgattg agcttgtact tgcccgtcaa
     6061 cacgccgcgc gcgatcggac tgtagggcac gacgccgagc ccgaaatggc gggcagccgg
     6121 caatagctcg acttcgggct ggcggttcac gagattgtag tagggttgca gtgcggtcgg
     6181 tgccggcacg ccggcgtcgc ggcaaaggtg atgaatatgc gggatgtgcc acgcgcgcac
     6241 gttcgagagg gcccactcgc ggattttgcc ctgcctgatg aggtcgccga aggtcagcac
     6301 cgtctgctcc cagctcgtgc cgggatcgag cttgtggatg tagaggatgt cgagcgcctc
     6361 gatgccgagg cgtcggaggc tcgcctcgca tgacatcatg acgtggcggc gcgtcagtcc
     6421 gcgatcagca acgccgggcc cgatcgggtt gccgaccttg gtggcgacga tccagcggtc
     6481 gcgcttgccc ttgaggccgc ggccgatcac ttcttccgag cggccctcgg tgtaggcgtt
     6541 ggcgctgtcg aggaaattga cgcccttggc gagggcgtca tccatgatgc ggtgcgcctc
     6601 agcttcgggc gtcggcccgc cgaacatcat ggttccgagg cagaggcggg agacggtgag
     6661 gccgctgtgg ccgagagtga cggttttcat ggcggctcca gattggatcg agggcgctgc
     6721 ccctcaagct ccgtgcgtct gtgatgttgc gtcgtcaggc tgttgcaagt ccagctcata
     6781 tgaggtcact cggtcacgac ctgtgaagcc gatggtgact gcgtcgcagc aatgcggggt
     6841 ggagagcaac tggaggcgaa cgagcagcca gtaggatgac gaacccgttt cggtcgggtg
     6901 gcgtggcgag cctgcggagc tgtggtgaag cccggaccaa gcccggtgcg ctccttcgcg
     6961 aatgctggac tgttcgcact atacccaacc tggcaagccg cgagacgcta tcgatgaaga
     7021 agccaccgac tggggagccg tatgcgggaa tgccgcccgt acggttcgga gggagggggc
     7081 gatgaaagcc gatccctacc cctatctcct tcttgatatc gaagggggcg ggttcgggag
     7141 tgcaggggca ttgtcgattg cgcgccttcg cgcgcggtgc cccactggct gcgaagcggc
     7201 catcaccccg cgcgcgcctt cgcgtagcga gcgagccgct cctcgagggt gcccgtgtgc
     7261 agctcgaaca tgtggttgtc gtgatcgtgg aaatagatcg agcgaccttc gcccgcgacc
     7321 cgagcccgcc cctctcgtat gtcgaggccg agcgaccgca cccgcccgag gcagtcgtcg
     7381 tagtccgcct cgcttatttt gaaggcgatg tgattgtagg agcgggtcgg cagcggctcg
     7441 ccctccatca ccgcgatcca gagactgccc gcgagataga accgctcctt cgacagggag
     7501 aatgtcgcgg tcccgctgtc gtagaccttc tcggcgccga gcacggagac gaggatcgcc
     7561 tccatccggt cgaggtcgcg gacgataaag gtcatgtggc tcagaccctc gatgcggctc
     7621 atcggtcgtg tcctgcagcg atcgcctcct cgatgatgcc gagggctgcc atcatctgat
     7681 cctccgtgat cgtgagcggc ggggtgagcg tcagcacgct tcccattgtc gtcttgaacg
     7741 agaggccgcg gtcgagcgcg cggtagagca ccgcttccgc aagatcgttg tccggagcct
     7801 tgctgtcgcg atcgcggacg agatcgatgc cgatcagcag gccgcggccg cggatgtcac
     7861 cgacagcggc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgacccgcg
     7921 ctgcattctc gacgagccgc tcgtcctcga tgatctcgat cgtcgtcagc gcggctcggc
     7981 aggtgacagg attcttctcg tgcgtgtaat ggccgaaggc atagtcggcg ccgacgtcga
     8041 gatcgggccg cgtcaccacg gcggcgatcg gcaggatgcc gccgccgagc gccttcccca
     8101 tcacgagaat gtcgggcgcg acgccatcat gctccgaggc gaacatgcgt ccagtcttgc
     8161 cgagtccggt cggaatctcg tcgaagacga ggagcgcgcc atgccggtcg caggcggcgc
     8221 ggacggactg ccagaagccc ggtggcggaa tgtagggaac tgcccgcgcg ggctcggcga
     8281 caacgcaggc gacatcggtc tcgcgcgaca ggacgtagtc gaccatgcgc gcgcaggcga
     8341 gcttgcagcg ctcgagatga ggctcgcctg aagcgtcgac gtcgtggccg taggcgcagc
     8401 gatagcaggc gaacggcgcc acgtgctggt cacccgagat cagaggcgcg gctggacccg
     8461 atcggaacag cgcctcgccc gagagcgccg cggcgccgaa gcccgcgccg tgaaaggcat
     8521 cccagaacga gagcgtcttg tgccggccgg tcgccgtgcg ggcaatcttc acagcgacct
     8581 cgacggcatc cgagccgccg gtcgtgaaca gcatcttcga gagcccgccc ggcgcgatcg
     8641 aagccagctt cttggccagc gccacggcag gcgcgttggc gaaacggcgc ggcgcgaacg
     8701 gcagctcgtc c
//
LOCUS       DAORNH010000197         8650 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6051_length_8650_cov_2.763700, whole genome shotgun sequence.
ACCESSION   DAORNH010000197 DAORNH010000000
VERSION     DAORNH010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6051_length_8650_cov_2.763700"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..1382
                     /locus_tag="PK264_17445"
     CDS             453..1382
                     /locus_tag="PK264_17445"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRD77693.1"
                     /translation="MTGPRRMSGMVAKLTIAGILGATASAILQADTAAAQGEVIMRKR
                     LPMEIVGGTPTRNDQHPWQIALMFVRDGQLRQFCGGSLVAPNWVLTAAHCVTADNEFK
                     ETDGHIPAEVMRVMTGATNVVARDGEVHEVEAVHVHSDYRVPNAPIADLLRTVDRAGA
                     NVNRNDIALIKLKTAATRGRPIALAPDDHSLNPGAMVEITGWGDTREGGRASAVLLRA
                     QVPYVAYDTCNGTAMYSGRLAEGMICAGLAVGGTDTCQGDSGGPMVHHSDRGAVLVGV
                     TSWGQGCARANRPGVYSSVTHFRAWITSTMTFQ"
     gene            1537..2331
                     /locus_tag="PK264_17450"
     CDS             1537..2331
                     /locus_tag="PK264_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRD77694.1"
                     /translation="MEIVGGTPSPITRHPWQVSIIMKSGEREFHFCGGSLVRPDWVLT
                     AAHCLAMPEVLQRHNGPVPAEMLKIRAGATTVGGTEGEIIEVETIHIHEGWMQFPTGN
                     LADLVRTPDKPGPGTMANDIALLKLKRPSTSGRPIELANDTLALGAGDEVEVTGWGAI
                     SEGGPGSRQLLRAAPRFIAFENCNGAARYDGNLTDGMICAGTDQGGVDTCQGDSGRPM
                     VRHTPAGPRLIGVTSFGIGCARANRPGVYSSVQYFRTWITTTIAFR"
     gene            complement(2381..3559)
                     /locus_tag="PK264_17455"
     CDS             complement(2381..3559)
                     /locus_tag="PK264_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77695.1"
                     /translation="MSRRLRQGLINAGLVVGSLAAMLALLEVTLFTYVLKPDDVLRNV
                     TVNGVVRYEPGTRATFRHPDGRETLVTINADGWNSTKPAYKVERTPGVLRVAVVGDSY
                     VHGSFVNVDEGFPEVLEKSLARSSTPAEVYRFGMDGAPLSQYLHVLRHEVGRFKPDVV
                     VVLLIHNDFDETYRFLQTRYASSFLKLGVNTAGQVIEFPPSDFTPGLADRFRDLRTFR
                     YLYYKTNAYLRLKSLVSRYWWGGNEEWSPEFVSSGVDIRRVSDHDKNDFYALHVMREM
                     KSVAEANGIKLLFVMDGVREAVYEGIAPHKYEVGRLNLIAADVTRRLALPFLDLQETF
                     RADYERRRERFEFSYDWHWNALANRLAGEAIARALVTDPRLLGPKASTAASETLPTTR
                     "
     gene            complement(3644..4450)
                     /locus_tag="PK264_17460"
     CDS             complement(3644..4450)
                     /locus_tag="PK264_17460"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD77696.1"
                     /translation="MSELYITSESYSAPTRRGSRSVVLCVPGPTTAQLSAHLDIKGLR
                     PVICSHPDLIDHSLSERGREAVILDVSGLGGLACDRLRRMRDRTSAIIVALVPANDSL
                     ARMAALDAGADACFSKPVEPGEVVTVLRALLRRVGMTRSDQARQPIDQSRRVRFGRMV
                     LDMDASRMTGPNGARVTITALELQVLKMFASHPNKALTRDEIAELAHGRRWSPLDRSL
                     DIRISRLRAKIEENPDEPRVIVTVRGIGYRFDADGDQSTQERASADLTIR"
     gene            complement(4746..4949)
                     /locus_tag="PK264_17465"
     CDS             complement(4746..4949)
                     /locus_tag="PK264_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77697.1"
                     /translation="MKKLILSIVAAAAIVPAASFISTDKAAAGDYGYGHGPRCFTVWV
                     RGHWVSDGYSKYFVPGRYVTRCR"
     gene            complement(5114..5326)
                     /locus_tag="PK264_17470"
     CDS             complement(5114..5326)
                     /locus_tag="PK264_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77698.1"
                     /translation="MKTMIPALALATLAVTTSLVSSTPAEARRGFGFGSSAPRCFTHY
                     EPNIRWCGIRNGKNGCWKGRYVVICK"
     gene            5574..6578
                     /locus_tag="PK264_17475"
     CDS             5574..6578
                     /locus_tag="PK264_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77699.1"
                     /translation="MSFHLSRRQALVGAASIASAGALGGLSPTASAKGPMLKSQAPYY
                     YRFGYGKAQVTMVSDGPLPLGDPSSNFLGASKDEITKMLTDNYLSPTSVVLEQNIPIV
                     NTGTHLVLFDTGMGFQKMFGPTTGRLLASMREAGIQPGQIDAIVISHAHIDHIGGMAD
                     ARGRRLFPNAKVYISKEDFDFWTDEKKLTDDKVKAFVKHARDNLMPYRDRIVFVKDGQ
                     EFLPGITAIAAPGHTIGHMIFMVQSDNKKLCFIGDLTHHQILLVERPRLEFAYDTDPK
                     QSAATRVRLLDMLAKDKIPLLAFHFPWPGYGYVSKHGDGFRYHATPMEIVTIPPKKKA
                     "
     gene            6729..7034
                     /locus_tag="PK264_17480"
     CDS             6729..7034
                     /locus_tag="PK264_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HRD77700.1"
                     /translation="MPARIFKPAKTAMQSGSARTKDWVLEAEPSAAREIDPLMGWTAS
                     SDTSAQVKLTFDTKEDAIAYAERNGIGYVVVEPTGRKPIRKSYSENFKFGRIGAWTH"
     gene            7078..7305
                     /locus_tag="PK264_17485"
     CDS             7078..7305
                     /locus_tag="PK264_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRD77701.1"
                     /translation="MADKTLDTKGLNCPLPILKAKKAIADVPKGGTLEILATDPGAVA
                     DFQAFCRTTGHKLVEHSEASGVYKFLIQRNA"
     gene            7311..7814
                     /locus_tag="PK264_17490"
     CDS             7311..7814
                     /locus_tag="PK264_17490"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD77702.1"
                     /translation="MPLLIEDQVPFRLIEAEVKPDWVNSLGHLRAAHYVQIFDDAFMA
                     MFPGLGLTDARLMCGSTSPVLADLHATYLAELRTGDRVSVAAQFLDADERRVRLILLM
                     HALGVARLAATCELAIVNMDLTSRRPAQWSTEQTTILDRLKRAHASLPMPKQAGRSIG
                     PLTLAVR"
     gene            complement(7807..>8650)
                     /gene="cysC"
                     /locus_tag="PK264_17495"
     CDS             complement(7807..>8650)
                     /gene="cysC"
                     /locus_tag="PK264_17495"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HRD77703.1"
                     /translation="VVETVAATTSATVTLLKHRLDPDTLSELAARGLERGEIGVVNLS
                     FGAHMPFDGLEAVPQTSAFLLRERKGGPVVGRGAITFALRRATNIHRQSQPVDKLARV
                     RLKGHRPACLWFTGLSGSGKSTVTGLVDKALNARGKHTYILDGDNIRHGLNRDLGFTD
                     VDRVENIRRVAEVSRLFVDAGLITLVSFISPFRAERALARSLLSDGEFIEVFVDTPLA
                     VCEARDAKGLYRKARSGALKNFTGIDSPYEAPEQAELHLDGTKAADDLVEQVLSALRS
                     RDVI"
BASE COUNT         1563 a         2792 c         2839 g         1456 t
ORIGIN      
        1 cagctggatg accacgcgcc ccgtcttgag ggtgaggacg atggtgtcct cgggattgac
       61 ctgggcgccg acgggggccg gcgagaacca gagggcgagt gcgagggcga ggctcacgaa
      121 gagacgcgtc acgctttact ccttcgatga ttgaagtgga cttcgatgcg actggcttcg
      181 atcaggtgct gtcgcgtcgc cggaccgcac ctgccggggc aggcctcgat gcttcgatcc
      241 ggtaacgccg tcggccgaag gcgctggtcc aggcccctgg cctcggcaag aatggctctg
      301 ggatgaacaa ttcatgaagg cacgtcaagc ctcagcccga gcgccggcat tcggccgcca
      361 cactagcggc cctttgccct cgaccggcct tacggctatt ctgaggctcg atttcgacga
      421 accacattcc ggcgaacgcc atggaggcaa gcatgacagg tccgcggcga atgagcggca
      481 tggtcgccaa actgacgatt gccggcattc tgggtgccac tgcatcggcg atcctgcagg
      541 cggacacggc cgcagcccag ggcgaggtca tcatgcgcaa gcgcctccct atggagatag
      601 tcggcggcac gccgacgcgc aacgaccagc acccctggca gatcgcactg atgttcgtcc
      661 gcgatggcca gctgcgccaa ttctgcggcg gctcgctcgt cgcacccaac tgggtgctga
      721 cggctgcgca ctgcgtcacc gccgacaacg agttcaagga aaccgacggc cacatcccgg
      781 ccgaggtaat gcgcgtcatg accggcgcga ccaacgtggt ggcgcgcgac ggcgaggttc
      841 acgaggtcga ggcggtgcac gtgcatagcg attatcgtgt accgaacgcg ccgatcgccg
      901 atctcctgcg cacggttgat cgcgcgggcg ccaacgtcaa tcgcaacgac atcgcgctca
      961 tcaagctcaa gacggcagcg acgcgcggcc ggccgatcgc gctcgcgcct gacgaccaca
     1021 gcctcaatcc aggcgccatg gtcgagatca cgggttgggg cgacacgcgc gagggcggcc
     1081 gcgcgtccgc cgtcctgctc cgcgcccagg ttccctatgt cgcctacgac acgtgcaatg
     1141 gcacggcgat gtacagcggc cgactagccg agggcatgat ctgcgccggt ctcgccgtgg
     1201 gcggaaccga cacgtgccag ggcgacagcg gcggccccat ggtccaccac agcgaccgcg
     1261 gcgccgtgct cgtcggcgtg acgtcgtggg gacagggctg tgcccgcgcc aatcggcccg
     1321 gcgtctactc gagcgtcacg cacttccggg cctggatcac gtcgacgatg actttccaat
     1381 aggccgatcg agctggagcc tgccgcaatg atcgcattcc ggacactgtt gagcgcatcc
     1441 ccgctcccga tcctcgcagc cacggctgta gtcgccgcgc tcgtcggcgg ggtctccccg
     1501 ttgggggccc aggcccctgc caagaaggta ttgcccatgg agatcgtcgg agggacgccg
     1561 tccccgatca cccgtcatcc ctggcaggtg tcgatcatca tgaagtcggg cgagcgtgag
     1621 ttccatttct gcggcggctc gctggtgcgc cctgattggg tgcttacggc ggcgcattgc
     1681 ctcgccatgc ccgaggtgct gcagcgccac aacgggcccg ttccggccga gatgctgaag
     1741 atccgcgccg gtgccacgac ggtcggaggt acggagggcg aaattatcga ggtggagacg
     1801 atccacatcc acgaaggctg gatgcagttt ccgactggca atctcgccga cctcgtccga
     1861 acgcccgaca agccaggccc tgggaccatg gccaacgaca ttgcgctctt gaagctcaag
     1921 cggccctcga cgtcaggccg gccgatcgag cttgccaatg acacgctggc tctcggcgcc
     1981 ggcgacgagg tcgaggtgac cggctggggc gccatttccg agggcggccc aggctcgcgc
     2041 cagctgctgc gcgcggcacc gaggttcatt gccttcgaga actgcaacgg tgcggcgcgc
     2101 tacgacggca atctgaccga cggcatgatc tgcgccggaa cagaccaggg cggtgtcgac
     2161 acctgtcagg gcgacagtgg cagaccgatg gtacgccaca cgccggcagg cccgcgactg
     2221 attggcgtca catcgttcgg gatcggctgc gcgcgtgcca accggccagg tgtctattcg
     2281 tcggtccagt atttccgcac ctggatcacg acgaccattg ccttccggta ggctcgttgg
     2341 cctggattga cggcgagcgc gaggagccgc tcttcgcgct tcaacgtgtc gtcggcaggg
     2401 tctcgctcgc ggcggtcgat gccttcgggc cgagaagccg cggatcggtg acgagcgcgc
     2461 gggcgatcgc ctcgccggcc aaccggttgg caagcgcgtt ccagtgccag tcgtagctga
     2521 actcgaaccg ctcgcggcgg cgctcgtagt cggcccggaa cgtctcctgc aggtcgagga
     2581 atggcagcgc caggcggcgg gtcacgtcag cggcgatgag attgagccgg ccgacctcgt
     2641 atttgtgagg cgcgatgccc tcgtagacgg cctctcggac gccatccatc acgaacagga
     2701 gcttgatgcc gttcgcctcc gcgaccgact tcatctcccg catcacgtga agggcgtaga
     2761 aatcgttctt gtcgtgatcg gagacacggc ggatgtcgac gccagacgag acgaactcgg
     2821 gcgaccattc ctcgttgcct ccccaccagt agcggctgac aaggctcttc aagcggaggt
     2881 aggcattggt cttgtagtag agatagcgga acgtcctgag gtcgcggaag cgatcggcga
     2941 gccctggcgt gaagtccgac ggcgggaact cgatcacctg cccggccgtg ttgacgccga
     3001 gcttcaggaa gctcgaggcg tagcgcgtct ggaggaagcg ataggtctcg tcgaagtcgt
     3061 tgtggatgag cagcacgacg acgacatcgg gcttgaagcg gccaacctcg tggcggagca
     3121 cgtggagata ctggctgagc ggtgcgccat ccatgccgaa gcggtagacc tcggctggtg
     3181 tcgatgaccg tgcgaggctt ttctccagga cctccgggaa cccctcgtcc acgttgacaa
     3241 agctgccgtg gacataggaa tcgccgacga cggcgacgcg caggacgccg ggcgtccgct
     3301 cgaccttgta ggccggtttc gtggagttcc agccgtccgc attgatcgtg acgagcgtct
     3361 cgcgcccgtc cggatggcgg aaggtcgctc gcgtgcccgg ctcgtagcgg acgacgccgt
     3421 tgaccgtcac gttccggagc acatcgtccg gtttcaagac atatgtgaaa agcgttacct
     3481 cgagcagcgc cagcatcgcc gccagcgagc cgacgacgag gcccgcgttg atgagcccct
     3541 gtcgcaatct tcgggacatt gcgctctgcg ctcccacttt ggaccactac ctcacgggta
     3601 atggtcgacg aggggagcaa gcacgatgcc agttggcggc acttcaccgg atcgtgagat
     3661 cggccgaggc gcgttcctga gttgactggt cgccgtcggc gtcgaaacgg tagccgatac
     3721 ctcgcacggt cacgatcaca cgcggctcgt ccgggttctc ctcgatcttc gccctcagtc
     3781 gcgagatgcg gatatcgagg ctgcgatcga gcggcgacca gcggcgaccg tgcgccagct
     3841 cggcaatctc gtcgcgcgtc agtgccttgt tgggatgaga cgcgaacatc ttgagaactt
     3901 ggagctcgag cgcggtgatc gtaacccggg cgccgttcgg gccggtcatg cggcttgcgt
     3961 ccatgtcgag caccatgcgg ccgaagcgga cgcggcggga ctggtcgatg ggctggcgcg
     4021 cctggtccga ccgggtcatg ccgacgcgcc gcaggagggc acgcagaacc gtcacgacct
     4081 caccgggctc gaccggcttc gagaagcagg catcggcgcc ggcatcgagc gctgccatcc
     4141 gcgcgaggct gtcgttggcc ggcacgaggg cgacgatgat cgccgaggtg cggtcgcgca
     4201 tgcgccgcag gcgatcgcaa gcaaggcccc cgagcccgct cacgtcgagg atcacggcct
     4261 cgcggccgcg ctcggacagc gagtgatcga tgaggtcggg atggctgcag atgacagggc
     4321 gaagcccctt gatgtcgaga tgggctgaca attgtgccgt cgtagggcct ggcacgcaaa
     4381 gaacgacgct gcgggatccg cggcgcgtcg gcgccgagta gctttcgctg gtgatgtaga
     4441 gttcagacat gacctcggct tcccacatcc aatgtcattc gtgccgcagc ggcggtgccg
     4501 gcgacgagcg cgtttccgcg gccggtgtgg cgagcggagt gcagggcgta gtcacattcg
     4561 tggagatcgg ctgatcgtgc agtgccctgc ggcacaaggc ttcgatcaag accgggatcg
     4621 atgaggcgtt gagttgggcg agcctcgggg ctcggcagtg gaaaggccac cctccagggg
     4681 gaggaggatc ccggagggtg gccatcggcc tgatccgttc ggtcaggcag tcgaggcggc
     4741 tccggttagc ggcagcgcgt cacgtagcgg ccggggacga agtacttgct gtagccgtcc
     4801 gacacccagt ggccgcggac ccacacggtg aagcagcgcg ggccatggcc gtagccatag
     4861 tcgccggcag cggccttgtc ggtcgagatg aacgaggcgg cggggacgat cgcggcggcg
     4921 gcgacgatcg agaggatgag cttcttcact tgaattctcc gttcaggggg ttgagcaggg
     4981 ggacttcccg gcatgcctca gagatgctca ccccagagcg gaaatttcgc gagccctgcc
     5041 gccccgaata aaatcgcccg cgagacgagg cttgcgggcg atcaacgacg aggcggggac
     5101 gcagacagcg ctatcacttg cagatgacga cgtagcggcc tttccagcag ccgttcttgc
     5161 cgttgcggat gccgcaccag cggatgttcg gttcatagtg cgtgaagcag cgcggcgcgc
     5221 tgctgccaaa gccgaagccg cggcgggcct cggcgggcgt gctcgacacg aggctcgtcg
     5281 tcaccgcgag ggttgctaat gcgagtgcgg ggatcatggt cttcacggca tatcctttcc
     5341 tgaatgacag caagctgaga gccgatgaga cgggctgtct gaacagcttc aactaaatga
     5401 ccgttgttca cggcagtctc gttttaatcc gatcactgta ggggttaacc atccccgtgc
     5461 cacacatgat ccaaggatag gaccttcgcc gaaatgatgg ggagtcttgc cctctccgcg
     5521 tccctggttc acagtcgaac tcggacgtgc attggaggaa atggaggtct cgaatgtctt
     5581 tccatctatc tcgccggcag gcacttgtcg gcgccgcctc gatcgcgagt gccggcgcac
     5641 tcggtggcct ctcgccgaca gcatcagcaa aaggcccgat gctcaagtct caggcgccgt
     5701 actattaccg cttcggctac ggcaaggccc aggtgaccat ggtttccgac ggcccgctgc
     5761 cgctcggcga tcccagctcg aacttccttg gtgcgtcgaa ggacgagatc accaagatgc
     5821 tgacggacaa ctatctttcg ccgacaagcg ttgtgctcga gcagaacatt ccgatcgtca
     5881 acacaggcac gcacctcgtg ctgttcgata cgggcatggg cttccagaag atgttcggcc
     5941 cgaccacagg gcggctcctc gccagcatgc gcgaggccgg catccagccg ggccagatcg
     6001 atgcgatcgt catctcgcat gctcacatcg accacatcgg cggcatggca gatgcgcgcg
     6061 gccggcggct cttcccgaac gccaaggtct acatttccaa ggaggatttc gacttctgga
     6121 cggacgagaa gaagctcacc gacgacaagg tgaaggcctt cgtcaagcac gcccgcgaca
     6181 acttgatgcc ctatcgcgac cggatcgtgt tcgtgaagga cggccaggag ttcctgccgg
     6241 gcattacggc aatcgccgcg ccgggacaca ccattggcca catgatcttc atggtgcagt
     6301 cggacaacaa gaagctctgc ttcatcggcg acctcacgca ccatcagatc ctgctcgtcg
     6361 agcggccacg cctcgagttc gcctacgaca ccgaccctaa gcagtcggcc gcgacccgcg
     6421 tcaggctcct cgacatgttg gcaaaggaca agatcccgct ccttgccttc catttcccgt
     6481 ggcccggcta cggctatgtc tccaagcacg gcgacggctt ccgctatcac gcgacgccga
     6541 tggagatcgt aacgatcccg ccgaagaaga aggcctgacg atcggacgtc caaggcgtgc
     6601 cccgccagag atggcggggc atttgcttgt cggccgaacg tcacaggcgc ggcggcacct
     6661 cgccaccttg agcaacccgc aaggctcggc tagaaaagtc gcgtcacgag atcacagaga
     6721 cgaaatcgat gcctgcacgg atcttcaagc cagccaagac agccatgcag tctggctccg
     6781 cgcgcaccaa ggactgggta ctagaggccg agccgtcggc cgcgcgcgag atcgacccgc
     6841 tgatgggctg gacagcctcg tcggacacgt ctgcgcaggt gaagctgacc ttcgacacca
     6901 aggaggacgc catcgcctac gccgagcgca atggcatcgg ctatgtcgtc gtcgagccga
     6961 ccgggcgcaa gccgatccga aagtcatact ccgagaattt caagttcggg cgcatcggcg
     7021 cctggaccca ctgacccgca gctcatctac cgccgccagt caaccaggga tcagatcatg
     7081 gccgacaaga cactcgacac caagggcctc aactgcccgc tgccgatcct gaaagccaag
     7141 aaggctatcg cggacgtgcc caagggcggc acgctcgaga tactcgccac tgaccctggc
     7201 gccgttgccg atttccaggc cttctgccgc accaccggcc acaagctcgt cgagcactcg
     7261 gaggctagcg gcgtctacaa gttcctcatc cagcgcaacg cctgacggcc gtgccgctcc
     7321 tgatcgagga tcaggtgccg ttccgcctca ttgaggccga ggtgaagccc gattgggtca
     7381 acagcctcgg ccacctgcgc gcggcgcact acgtgcagat tttcgacgac gccttcatgg
     7441 cgatgtttcc ggggctcggc ctgaccgacg cgcggctcat gtgcggctcg accagccccg
     7501 tcctcgccga cctccacgcg acctatctcg ccgaactccg gacaggcgat cgcgtgagcg
     7561 ttgcggcgca gttcctcgat gccgatgagc gccgcgtgcg tctcatcctt ctgatgcatg
     7621 cgctcggtgt agcgcgcctc gccgcgacat gcgagctggc gatcgtcaac atggacctca
     7681 cgagccgccg gcccgcacaa tggtctaccg agcagacgac catcctcgat cgcctgaagc
     7741 gcgcgcatgc gtcgctgccg atgccgaagc aggccggccg ctcgatcggc ccgctgacgc
     7801 tggccgtcag atgacgtcgc gcgacctgag tgcgctcagg acctgctcga cgagatcgtc
     7861 ggcagccttc gtgccatcga ggtgaagctc cgcctgctct ggcgcctcgt aggggctgtc
     7921 gatgcccgtg aagtttttga gcgcgccgga tcgtgctttc cggtagaggc ccttggcgtc
     7981 gcgcgcctcg cagacggcga gcggtgtatc gacgaagact tcgatgaatt cgccgtccga
     8041 gagcagcgat cgggccaggg cgcgctcggc acggaacgga gagatgaacg aaacgagcgt
     8101 gatcaggccc gcatcgacga acagcctcga cacctcggcg acccggcgga tgttctcgac
     8161 gcgatcgacg tcggtgaagc caaggtcacg gttgaggcca tggcggatgt tgtcgccgtc
     8221 gaggatatag gtgtgcttgc cgcgcgcatt gagcgccttg tcgacaaggc ccgtgaccgt
     8281 cgacttgccc gagcccgata gccccgtgaa ccagaggcat gccggtcggt gccccttgag
     8341 acgcacccgc gccaacttgt caaccggctg cgactggcga tggatgttcg tcgcccgccg
     8401 gagcgcgaag gtgatggcgc cgcgcccaac caccggtccg cccttacgtt ccctgagcag
     8461 gaaggcgctg gtctgcggaa cagcttcgag gccatcgaac ggcatgtgag cgccgaacga
     8521 gaggttgacg acgccgatct cgccgcgttc gaggccgcgg gccgcaagct cggagagcgt
     8581 gtcgggatcg agacgatgct taagcagcgt cactgttgcg ctcgtcgtgg ccgcaacggt
     8641 ctcgaccaca
//
LOCUS       DAORNH010000198         8472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6190_length_8472_cov_2.518475, whole genome shotgun sequence.
ACCESSION   DAORNH010000198 DAORNH010000000
VERSION     DAORNH010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_8472_cov_2.518475"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>232)
                     /locus_tag="PK264_17500"
     CDS             complement(<1..>232)
                     /locus_tag="PK264_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77704.1"
                     /translation="EIETDKATMEVEAVDEGIVSQLLVPAGTEKVKVNTPIAVLIVAG
                     EKPGAGGPPPIPPAQPTAASPPPPLRAATPPPL"
     assembly_gap    233..348
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            770..3214
                     /locus_tag="PK264_17505"
     CDS             770..3214
                     /locus_tag="PK264_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77705.1"
                     /translation="MLLVASLACTVVLTVAIADSGPELIGGLRLAHVLAMSIAAGGVT
                     LAFAIAWRWSHGRHERVRRLERELTTVRRSLAASEAIAKAEPQVLFVWEQDGGPKVLT
                     NTLTNVPGLPEDIVEFEHFGAWLEPTSAQQLKAGLDQLFAAGRPFNQLIRTVGGGNLE
                     ADGRVAAGRPMLRFRDIAGHKRDLARIVDQHRQLAHDIRASRAVLNLVTMPVWFRGSS
                     GRIEWVNRAYVAAVEAADDAEVRERQIEFLESRQRDEIARALGASGSFSKRLAITTAG
                     VRRSYDVMAQAIEGGAVCAAIDVTAIETAASTLDRQLGAKERTLDRVSTAIASFRRDQ
                     RLAYYNEAFQKLWRLDGDWLATKPTHGEILDRLRELSRLPEAASYRDWRTTVITGKLD
                     ADGYWHLADGTAIRVMAEESADGGLTYLYEDETESIALASRYNALIDVQRETLDHLRE
                     GVALFATDGRLQLYNSSFASIWKLSRQLLDEGPHVDEIIRQCRVLADDRAIWSDLSRA
                     VTVISDNRRSLEGKITRPDQSVIEYATLPLPDGATMLTFTDVTEKLSYERALIERNEA
                     LVAADRLKSQFISHVSYELRTPLTNIIGFSELLESHRTGPLQPKQREYLGDIQASSKT
                     LLAIIDDILDLATIDAGALELRLGPVKVRSIVEAAILGVRDRAQRANIKLDIAIDPKA
                     DEVIADEARIRQVLFNLVSNAIGFSNPGGAVRVATWREAGMIAFSVEDKGVGIPKDQQ
                     RSVLERFVSRSRGSRHRGAGLGLSIVKSLVELHGGDMSLDSEPGKGTSVVVRFPENGQ
                     RPRPPRSGDTDEIDAG"
     gene            3424..>4261
                     /locus_tag="PK264_17510"
     CDS             3424..>4261
                     /locus_tag="PK264_17510"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRD77706.1"
                     /translation="MNVVIVESAAKAKAINKYLGSSYKVIASYGHVRDLPAKDGSVEP
                     DNDFEMHWSLDDDRSKKVMREISEAVRKADKLILATDPDREGEAISWHILEVLNQKRI
                     LKSGTPVERVTFNAVTKQAVLEAMKAPRDIDSPLVEAYLARRALDYLVGFTLSPVLWR
                     KLPGARSAGRVQSVALRLVCDRELEIEKFKTDEYWTIEALLATAKAEEFRARLTTVAG
                     TSLRKLDIKDGTTANAIKAAIEGQDFRIVSVDKKGAKRNPYAPFTTSPLQMDASRKLG
                     FSA"
     assembly_gap    4262..4317
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4318..>4538
                     /locus_tag="PK264_17515"
     CDS             <4318..>4538
                     /locus_tag="PK264_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77707.1"
                     /translation="GMISGTCLTLSTIAPQIRDVISKEYGRNYTPFAREYKTKAKNAQ
                     EAHEAIRPTDPRRKPDDVRRYLEKDQAAL"
     assembly_gap    4539..4615
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4616..>4917
                     /locus_tag="PK264_17520"
     CDS             <4616..>4917
                     /locus_tag="PK264_17520"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRD77708.1"
                     /translation="DGKKYGLRATGSIIVFDGFLKVYEEGRDERHRVIEKGKVDTEGD
                     DDDSRRLPPLAAGDRLKDKGIDADQHFTQPPPRFTESTLIKRMEELGIGRPSTYAST"
     assembly_gap    4918..5022
                     /estimated_length=105
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5023..6112
                     /locus_tag="PK264_17525"
     CDS             <5023..6112
                     /locus_tag="PK264_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HRD77709.1"
                     /translation="EYDFTADLEGKLDLISDGQLVWKDVLRDFWRDFTASVGEIKDLR
                     VGQVLDALNDLLGPHIFPDKGDGSDPRRCPSCGEGRLSLKISGKTGAFIGCSKYPDCR
                     FTRQLAKSTGDGTTEAMNPDGKILGICPDTGLTVSLRNGRFGAYVQLGDTKDYDDGKP
                     KRASIPKGWDASTLDLERALKLLNLPRTIDNHPETGTPVLAGIGRYGPYILHDGTYVN
                     LPSVDEVFDIGLNRAVSMIADKKAGKGGSRFGRGAAVRQVLKELGEHPSEGGKIEVLN
                     GRYGPYVAHNKVNATVPRGKDPASLTVEEAIGLLTERIAKMAENPGKGKSRGGFKKKA
                     KPGKDEGSDAGAAKPAKKSAKSRKAAAE"
     gene            complement(6113..7642)
                     /gene="tsaE"
                     /locus_tag="PK264_17530"
     CDS             complement(6113..7642)
                     /gene="tsaE"
                     /locus_tag="PK264_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRD77710.1"
                     /translation="MTHDWKLASLDEPGIARLAELIALHARSGDALLLEGDLGAGKTT
                     FARAMIRAALADDDAEVPSPTYTLVQTYAGPRCEIAHYDLYRIADASEVAELGLDEAL
                     AKGIVLIEWPGRLWGVPAADTLEIRLADAGGDQRSVTLEARGSWQPRLARAEAMWAFI
                     PDAWRNARLGYLQGDASPRAYARLTDEGRSAILMNAPRMPDGPPIRNGLAYSRIAHLA
                     EDVRPFVAIGRELRRRGIAAPEIIAEDLDQGFLLIEDQGDLPFGRALAEGADQAVLWR
                     AAVDVLVALRAGGPPADMVIAPGTIHRLPGFDQGALAIETELLVDWYWPALYGVPCPA
                     DARAAFEAAWSQVFARLLAEPSGWLLRDYHSPNLMWRPERCGLARVGVLDFQDAMSGP
                     WAYDVVSLLQDARLDVPEALERDLLAHYKREAAHIDTGFDPEAFDFAFAALGAQRNTK
                     ILGIFARLAKRDGKPQYLAHLPRIWRYLERDLSALDLEGLKGWFDHHIPRSRRAERPA
                     A"
     gene            complement(7703..8410)
                     /locus_tag="PK264_17535"
     CDS             complement(7703..8410)
                     /locus_tag="PK264_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HRD77711.1"
                     /translation="MERTEVLRREVKALVFDQYGTIVDMQKGLTEIATPFLKKKGWDG
                     NPHQFVTWWRRTHFENSMIDALCDRGHTPYRQIGHRAVSYVMDRCAFAYTQDEVRWLV
                     SEIEKLKPFPDVIAALEKLRGAGYKLIILSNGDRDMLKAAGPHIGFPFDQVISVEEAG
                     YFKPHWKTYAKAAELSGLDRSSCLFVANHAFDCIGAKSYGMRTAFIDRRKRPFGETPH
                     QADLIVANFAELAQSLC"
BASE COUNT         1426 a         2788 c         2658 g         1246 t
ORIGIN      
        1 aagtgggggc ggtgtcgcgg cgcgaagggg aggcggcggg ctcgcggcgg tgggctgcgc
       61 cggggggatc ggcggcggac ctcccgcgcc cggtttctcg cctgcaacaa tcagcaccgc
      121 gatcggcgtg ttcaccttca ccttctcggt gccggccggt accaggagct ggctgacgat
      181 cccctcgtcg actgcctcga cctccatggt ggccttgtcg gtctcgatct cgnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg gcatgtggca
      361 gtgatcctgg cgatcgtggt tcgctagcgg gaggaggcgt tccgcggacc ttacgaccga
      421 ggcccgtcgc cgctcaagcg cattcgagcg cgcggccgcg acgaccgacc cggccggatg
      481 tttctggagt gcaatcggcg cgcgccgaga cacctgtgta caacatcgcc cctccggcag
      541 gctcgggcgg tcccctactg tgctttatcc acactggtca tcatcggcga agcacagcat
      601 actgcactga ttcgaattca actcgagatg ggcgcagccg ccatccgagc ccatcgcagg
      661 ttgttgtgcg tcgcgcgttg gccggagggt cggctttccc gatgcatgga cccagatgac
      721 ggcagccacg ccccgtaaca gagacatcgg atcgcgcggc gagatcatca tgctgctcgt
      781 cgcaagtctt gcgtgcacgg tcgtgctcac cgtcgcgatc gccgacagcg ggccagagct
      841 catcggcggc ttgcggctcg cacatgtcct cgccatgtcg atcgccgccg gcggcgtcac
      901 gctggcgttc gccatcgctt ggcgctggag ccacggccgc cacgagcgcg tccggcgcct
      961 cgagcgcgag ctcacgaccg tccgccggag tctcgccgcg tccgaggcga tcgccaaggc
     1021 cgagccgcag gtgctgttcg tctgggagca ggacggcggc ccgaaagtcc tcaccaacac
     1081 gctgacaaac gttccgggcc tgcccgagga cattgtcgag ttcgagcatt tcggcgcctg
     1141 gctcgagccg acctcggcgc agcagctcaa ggccgggctc gaccagctgt tcgcggcggg
     1201 gcggccgttc aaccagctga tccggaccgt gggcggcggc aacctcgaag ccgacgggcg
     1261 tgtcgctgcc ggccggccca tgctgcgctt ccgagacatc gccggccaca agcgcgatct
     1321 cgcacgcatc gtcgaccagc accgccagct cgcccacgac atccgggcga gccgggccgt
     1381 cctcaatctc gtcaccatgc cggtctggtt ccgcggcagc agcggccgca tcgagtgggt
     1441 caatcgggct tacgtcgcgg ccgtcgaagc cgccgacgac gccgaggtgc gcgagcgcca
     1501 gatcgagttc ctcgaatctc gccagcgcga cgagatcgcg cgcgcgctcg gcgcgtcggg
     1561 atcgttctcg aagcgcctcg ccatcacgac ggcgggcgtg cgccgctctt acgatgtgat
     1621 ggcgcaggcg atcgagggcg gcgccgtctg tgcagctatc gacgtcacgg cgatcgaaac
     1681 cgcggccagc acgctcgacc ggcagttggg cgccaaagag cgcacgctcg accgcgtctc
     1741 gaccgcgatc gccagctttc gccgcgacca gcgcctcgcc tactacaacg aggccttcca
     1801 gaagctctgg cgtctcgacg gtgactggct tgcgaccaag ccgacacacg gcgagatcct
     1861 cgaccggctg cgcgaactct cccgccttcc cgaggccgcg agctaccgcg actggcgcac
     1921 caccgtcatc accgggaagc tcgacgcgga cggctactgg catctcgccg acggcactgc
     1981 gatccgcgtc atggcggaag agagcgcgga cggcgggctc acctacctct acgaggacga
     2041 gacggaaagc attgcactcg ccagccgcta caacgcgctc attgatgtgc agcgcgagac
     2101 gctcgatcac ctccgcgaag gagtcgcctt gttcgcgacc gatggccgtc tgcagctcta
     2161 caactcctcg ttcgcgagca tctggaagct ctcgcgccag ctcctagacg aaggtccgca
     2221 cgtcgacgag atcatccgcc agtgccgcgt gcttgccgat gatcgcgcga tctggtcgga
     2281 tttgtcccgc gccgtcaccg tgatctccga caaccgccgc tcgctcgaag gcaagatcac
     2341 gcgacccgac cagagcgtga tcgagtatgc gacgctgccg ctccccgatg gcgccaccat
     2401 gctgacgttc accgacgtca ccgagaagct ttcctacgag cgcgcgctca tcgagcgcaa
     2461 cgaggcgctc gttgccgccg accgcctgaa gagccagttc atcagccacg tgtcctacga
     2521 gctgcgcacg ccgctcacca acatcatcgg cttcagcgag ctgctcgaga gccatcgcac
     2581 ggggccgctc cagccgaagc agcgcgagta tctcggcgac atccaggcct catcgaagac
     2641 gctgctcgcc atcatcgacg acatcctcga cctcgcgacg atcgatgcgg gcgcgctcga
     2701 gctgaggctc gggcctgtca aggtgcgctc gatcgtcgag gccgcgatcc tcggcgtgcg
     2761 cgatcgggcg cagcgcgcca atatcaagct tgacatcgcc atcgacccca aggccgacga
     2821 ggtgatcgcg gacgaagcgc gcatccgcca ggtgctattc aatctcgttt cgaacgcgat
     2881 cggattctcg aacccgggcg gcgcggtgcg tgtggcaacc tggcgcgaag ccggcatgat
     2941 cgccttctcg gtcgaggaca agggcgtcgg catcccgaag gaccagcagc gcagcgtcct
     3001 cgagcgcttc gtctcgcgga gccgcggctc gcgccaccgt ggcgccggcc tcggcctctc
     3061 gatcgtcaag agcctcgtcg agctgcacgg cggcgacatg tccctcgaca gcgagccggg
     3121 gaaaggcacg agcgtcgtcg ttcgcttccc agaaaacggc cagcgcccca ggccgccgcg
     3181 gtccggggac acagatgaga tcgacgccgg atgacgtggt gggctgttct gttcgctgtg
     3241 ttcgaagcac atccggctga ccgcctgatt gcaccgccaa attgcatggc accgaccgcg
     3301 ccccctttcc accgcggcgg cgcgatcgtt tgacagccgc gccgtcctcg accatggtcc
     3361 gcgccgatat ccgaacccat aagaacggcc ctgcctcacg ctctcagggc cggcggaagg
     3421 ctcatgaacg tcgttatcgt ggaatcggcc gccaaggcca aagccatcaa caagtatctc
     3481 ggctcctcct acaaggtgat cgcgagctac ggccacgtgc gcgatctccc tgccaaggac
     3541 gggtcggtcg agcccgacaa tgacttcgag atgcactggt cgctcgatga cgaccgctcg
     3601 aagaaggtga tgcgcgagat cagcgaggcc gtgaggaagg ccgacaagct catcctcgcg
     3661 accgaccccg accgcgaggg cgaggcgatc tcctggcaca tcctcgaggt gctcaaccag
     3721 aagcgcatcc tgaagagcgg cacgcccgtc gagcgcgtca cattcaacgc ggtcaccaag
     3781 caggccgtgc tcgaggccat gaaggcgccg cgcgacatcg acagcccgct ggtcgaggcc
     3841 tatctcgccc gccgcgcgct cgattacctc gtcggcttca cgctctcgcc cgtgctctgg
     3901 cggaagctcc caggtgcgcg ttcggccggt cgcgtgcagt cagtggcgct ccgcctcgtc
     3961 tgcgaccgcg agctcgagat cgagaaattc aagaccgacg agtactggac gatcgaggcc
     4021 ttgctcgcca ccgccaaggc cgaagagttc cgcgcgcgcc tgacgaccgt cgccgggacg
     4081 agcctcagga agctcgacat caaggacggc accaccgcca acgccatcaa ggccgcgatc
     4141 gagggccagg atttccgcat cgtctccgtc gacaagaagg gggcgaagcg caacccctat
     4201 gcgcccttca ccacctcgcc cctgcagatg gacgcgagcc gcaagctcgg cttctcggcc
     4261 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg
     4321 catgatctcg ggcacctgtc tgaccctgtc gaccatcgcg ccgcagatcc gcgacgtgat
     4381 ctccaaggag tacgggcgca actacacgcc cttcgcacgc gagtacaaga ccaaggcgaa
     4441 gaacgcccag gaggcgcacg aggcgatccg cccgaccgat ccgcgccgca agccggacga
     4501 cgttcgccgc tacctcgaga aggaccaggc cgccctttnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngacgg
     4621 caagaaatac ggcctccgcg ccaccggctc gatcatcgtt ttcgacggct tcctcaaggt
     4681 ctatgaggag ggccgcgacg agcgccaccg cgtcatcgag aagggcaagg tcgataccga
     4741 gggcgatgac gacgacagcc gccgcctacc cccgctcgcg gccggcgatc gcctcaagga
     4801 caagggcatc gacgccgacc agcacttcac acagcccccg ccgcgcttca ccgaatcgac
     4861 cctcatcaag cgaatggagg agctcggcat cggccggccg tccacctatg cctcgacnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgagtacg atttcacggc
     5041 cgacctcgaa ggcaagctcg acctgatctc cgacggccag ctcgtctgga aggacgtgct
     5101 gcgcgacttc tggcgcgact tcacggcgtc cgtcggcgaa atcaaggatc tgcgcgtcgg
     5161 ccaggtgctg gatgcactga acgaccttct gggcccccac atctttcccg acaagggtga
     5221 cggctcggac ccgcgccgct gcccctcctg cggcgagggg cggttgtcac tgaagatctc
     5281 gggaaagacc ggcgccttca tcggctgctc gaagtatcct gactgccgct tcacgcggca
     5341 gctcgccaag tcgaccggag acggcacgac cgaggccatg aacccggacg gcaagatcct
     5401 tgggatctgt cccgacaccg gtcttacggt ctcgctgcgt aacggccgct tcggtgccta
     5461 tgtgcagctc ggcgacacca aggactacga cgacggcaag ccgaagcgcg cctcgattcc
     5521 aaagggctgg gacgccagca cgctcgacct cgagcgggcg ctgaagctgc tcaatctgcc
     5581 gcgcacgatc gacaatcatc ccgagaccgg cacgcccgtg ctcgccggca tcgggcgcta
     5641 tggcccctac atcctgcacg atggcaccta cgtgaacctg ccgtcggtcg acgaggtgtt
     5701 cgacatcggc ctcaaccgcg ccgtgtcgat gatcgccgac aagaaagcgg ggaagggcgg
     5761 ctcccgcttc ggtcgtggtg ctgcggtgcg ccaggtcctg aaggagctcg gcgagcatcc
     5821 ctccgagggc ggcaagatcg aggtgctgaa cggccgctac ggcccctatg tcgcgcacaa
     5881 caaggtcaac gccaccgtgc cgcggggcaa ggatccggcg agcctaaccg tcgaggaggc
     5941 gatcggcctc ctcacggagc gcatcgccaa gatggccgag aacccgggga agggcaaatc
     6001 gcgcggcggc ttcaagaaaa aggcgaagcc cgggaaggac gagggcagcg acgcgggcgc
     6061 ggcaaagccc gcgaagaaga gcgcgaaatc caggaaggcc gcggccgagt gatcaggccg
     6121 ccgggcgctc cgcgcggcgg ctcctcggga tgtggtgatc gaaccagccc ttgagcccct
     6181 cgaggtccag ggccgagaga tcgcgctcga ggtagcgcca gatgcgtggc aggtgggcga
     6241 ggtactgggg cttgccatcc cgtttggcga ggcgcgcgaa gatgccgagg atcttggtgt
     6301 tgcgctgcgc gccgagagcc gcaaaggcaa agtcgaaggc ctccggatcg aaccccgtgt
     6361 cgatgtgtgc agcctcgcgc ttgtagtgcg ccaggaggtc tctttcgagc gcttcgggca
     6421 catcgagccg cgcgtcctgg aggagcgaga cgacgtcgta ggcccacggc cccgacatcg
     6481 catcctggaa gtcgaggacg ccgacgcgcg cgaggccgca ccgttcgggc cgccacatca
     6541 ggttcggcga gtggtagtcg cggagaagcc agccgcttgg ctcggcgagg aggcgcgcga
     6601 agacctgcga ccaggccgcc tcgaaagccg cccgcgcatc ggccgggcag gggacgccat
     6661 agagcgccgg ccagtaccag tcaacgagga gttctgtctc gatcgccagg gcgccttgat
     6721 cgaagccggg caggcggtgg atcgtccccg gcgcgatcac catgtcggcg ggagggccgc
     6781 cggctctcag cgctaccagc acatcgaccg cggcgcgcca gagcacggcc tggtcggccc
     6841 cttccgcgag cgcgcggccg aacggaagat cgccctggtc ctcgatgagc aggaagccct
     6901 ggtcgaggtc ctcggcaata atctccggcg ccgcgattcc tcgtcggcga agctcccgcc
     6961 cgatagcgac gaacggcctg acgtcctcgg cgaggtgtgc gatccggctg taggcgagcc
     7021 cgttacggat cggcgggccg tccggcatgc ggggcgcgtt catgaggatg gccgaccgcc
     7081 cctcgtccgt aagccgcgca taggcccgcg gcgaggcatc gccctgcagg tagccaagcc
     7141 gcgcattgcg ccacgcgtcg ggaatgaagg cccacatggc ctctgctcgt gcgaggcggg
     7201 gctgccagct gccgcgggcc tcgagcgtga ccgagcgttg atcgccgccg gcgtccgcga
     7261 gcctgatctc gagcgtatcg gcagccggga cgccccagag ccgccctggc cactcgataa
     7321 gcacgatgcc cttcgcgagc gcttcgtcga ggccgagttc ggcaacctcc gaggcgtccg
     7381 cgatgcgata gaggtcgtag tgcgcgatct cgcagcgcgg acccgcgtag gtctgcacta
     7441 gcgtataggt cggactcggc acctcggcat catcgtccgc gagggctgcc cgaatcattg
     7501 cgcgcgcgaa cgttgtcttg ccggcgccga ggtcgccctc gagcaggagg gcgtcgcccg
     7561 agcgcgcatg gagcgcaatc agctcggcca gacgcgcaat gcccggctcg tcaaggctcg
     7621 caagtttcca atcgtgtgtc atggtcggga cggaacagca gccgcgcgcc cgccgtcaag
     7681 tcgttgctcc gacgcggtgg gctcagcaga gtgattgagc gagctccgcg aagttcgcga
     7741 cgatcaggtc cgcctggtgc ggcgtctcgc cgaaggggcg cttgcgacga tcgatgaagg
     7801 cggtgcgcat gccgtaggac ttggcgccga tgcaatcgaa ggcgtggttg gcgacgaaca
     7861 ggcagctcga gcgatcgagg ccggagagct ccgcggcctt ggcgtaggtc ttccagtgcg
     7921 gcttgaagta gccggcctcc tcgacggaga tgacctggtc gaacgggaaa ccgatgtgcg
     7981 gtccggccgc cttcagcatg tcgcggtcgc cgttggagag gatgatcagc ttgtagcccg
     8041 cgccacgcag cttctcgagc gctgcgatca catcggggaa gggtttgagc ttctcgatct
     8101 cggagacgag ccagcgcacc tcgtcttgcg tgtaggcgaa ggcgcaacgg tccatcacgt
     8161 aagagacggc gcgatgcccg atctggcggt agggcgtgtg gccgcgatcg cagagcgcgt
     8221 cgatcatgga gttctcgaag tgagtgcgcc gccaccaagt cacgaactgg tgcggattgc
     8281 cgtcccagcc cttcttcttc aggaacggcg tggcgatctc ggtcaaccct ttctgcatgt
     8341 cgacgatggt gccgtactgg tcgaacacca gcgccttcac ctcgcgcctc aagacctcgg
     8401 tgcgttccat gacatccccg tgctcaacct cgacaaggca aaactgccag acgtgctgaa
     8461 atcgttccaa tc
//
LOCUS       DAORNH010000199         8469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6191_length_8469_cov_2.064535, whole genome shotgun sequence.
ACCESSION   DAORNH010000199 DAORNH010000000
VERSION     DAORNH010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6191_length_8469_cov_2.064535"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>273
                     /locus_tag="PK264_17540"
     CDS             <1..>273
                     /locus_tag="PK264_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD77712.1"
                     /translation="AVPRPLGAGLLLQPTGLAALDRLGLAGTALVRGQTIRGIDGKTV
                     LGRGVLAIDYADLDPRLYGVGIHRAALFQILYDAGQAERIALIADCP"
     assembly_gap    274..424
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <425..1136
                     /locus_tag="PK264_17545"
     CDS             <425..1136
                     /locus_tag="PK264_17545"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD77713.1"
                     /translation="FRFGAVWAPVPLPPDWPAQNRLVQRYRGARIMIGVLPIGTLAVG
                     DAPLAALFWSLPADSYQAWRHRGIDPWREEVGALWPDVAPLIAKLGSVDDLAFARYSD
                     LTTATPIADRLAIIGDAAHSTSPQLGQGANLALMDAEALSSAIAAHPNALEVSLQTYA
                     ASRRRHVRFYQFASRWLTPFFQSESRIAGAVRDLTFGPAARLGYVRRQMVATLAGLKP
                     GLVALPDLSGELSLPPAP"
     gene            complement(1114..2217)
                     /locus_tag="PK264_17550"
     CDS             complement(1114..2217)
                     /locus_tag="PK264_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD77714.1"
                     /translation="MTLHVRLDQDAPIPLDVAFDCAPGELLALVGPSGSGKTTILRAI
                     SGLMTPASGRIVSGESVWLDTSLSLALSPQQRRVGLVFQDYGLWPHRDALGNVMLAML
                     DQPSGEREGRARALLDLVNLAGLERRMPAALSGGQQQRVALARALARDPAVLLLDEPF
                     SAVDQMTRQRLQRELALIRHRLAIPIVLVTHDLDEAMMLADRIAVLQRGKTLQVGTPE
                     EVRLRPASSTVARLLGQTNIFSGRLTTSALAPATGRLDWGGIALEVAATGSVREGETV
                     DWLIPRDHVVLHRRGRPSLGERENPVVGTIRSLVILGEQASVEMTVEAVPGAVLNFRL
                     PTHAARRNGLERGAEITVSLLADGIHLMAPAAG"
     gene            complement(2214..2888)
                     /gene="modB"
                     /locus_tag="PK264_17555"
     CDS             complement(2214..2888)
                     /gene="modB"
                     /locus_tag="PK264_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRD77715.1"
                     /translation="MDWTAFRLSLSLALWTALLIVPTGVVIGRALARRRGTGKAIIEA
                     LLLLPLVLPPTVLGYGLLMALAPSSGFGRLIASLAGGPLVFTFTGILIASLIVNIPFA
                     VQPIVRAFESLPQSLGEAAAVSGLSPLQTFWRIELPLVWPGIVTAIALVVAHTLGEFG
                     VILMIGGSIPGETQTLSIALYDRVQAFRNAEAAVIAGVLVITSLVALALVNWTARRTD
                     PRRGRP"
     gene            complement(2896..>3312)
                     /gene="modA"
                     /locus_tag="PK264_17560"
     CDS             complement(2896..>3312)
                     /gene="modA"
                     /locus_tag="PK264_17560"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD77716.1"
                     /translation="SRLAGLAEALDTRAIHRFAIANPEHAPYGERAKAALMAAGVWEA
                     AQKRLVLGDTVSQAAQFVVSGAAEAGLVALSLVRSPMLEGRVSSAVVDDGLAAPLRQT
                     AVTLARARAPARAFAAYIDSEPARQVLDRFGFGRGA"
     assembly_gap    3313..3412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3413..3698)
                     /locus_tag="PK264_17565"
     CDS             complement(<3413..3698)
                     /locus_tag="PK264_17565"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRD77717.1"
                     /translation="MIASRRALIGGVAATVAAPCLVPGPSRAADDALIAAASDLRYAL
                     DEIAAAHVAVRRSSGAPPINLRITYGSTGNLARQIEQGAPFELFLAADDRI"
     gene            complement(3695..4216)
                     /locus_tag="PK264_17570"
     CDS             complement(3695..4216)
                     /locus_tag="PK264_17570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRD77718.1"
                     /translation="MGGQSKSNQSPVMEAHAYRAIMRHQAGAVTIIAVGKPGHRTGLT
                     ATAVCSVTDAPPTILVCINRKASAHAPIMAEKSFTVNMLARPQTDLALRFSGRAGVEG
                     EDRFDADAWTTLVTGAPILKGAIASLDCEVTEFRDMSTHSVFIGRVVAGTADETIDPL
                     LYFRGEFWDLVRR"
     gene            4381..>4586
                     /locus_tag="PK264_17575"
     CDS             4381..>4586
                     /locus_tag="PK264_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HRD77719.1"
                     /translation="MRGLAGLGAMLVALGLASPAAAEVATPWADGHKTRGRLVAGTVP
                     APGGPRILAGLEIELADGWKTYWRT"
     assembly_gap    4587..4754
                     /estimated_length=168
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4755..5252
                     /locus_tag="PK264_17580"
     CDS             <4755..5252
                     /locus_tag="PK264_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77720.1"
                     /translation="PIEIAPEAPGKPAEIKVVVELGVCKDICVPVTFNFELTVPDARA
                     LPASVHLAKAVDAVPRRAAGRRDDDPIIERQVAVLDGAKPSLTFHVAYPAGAVGADLF
                     VEAPDGAWVPLPAIKPDKDGRRVTFEIDLSTGVDLKDLIGKPLAVTVVGPTGALETST
                     VLKAK"
     assembly_gap    5289..5343
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5630..>6529
                     /locus_tag="PK264_17585"
     CDS             5630..>6529
                     /locus_tag="PK264_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004692047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRD77721.1"
                     /translation="MLSKVLIANRGEIALRIMRTAKRLGMATVAVYSEADRDALFVRH
                     ADEAVAIGPAPATESYLRLDRIIEAAQKTGADCIHPGYGFLSENADFPDACAKAGIAF
                     VGPSGEAMRKLGGKAAAKAIARAAGVTVVPGYDGDAQDAKTLAEHAGRIGYPVMIKAV
                     AGGGGRGMRLVTKPAEFAAALESAAREAAASFGNPRVLVEKVVVAPRHVEVQVFGDRH
                     GNVVHLFERDCSLQRRNQKVIEEAPAPGMSEGLRSAITGAAVKLAKAVGYEGAGTVEF
                     LVEGGTLSATAPWYFIEMNTRLQV"
     assembly_gap    6530..6562
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6563..7596
                     /locus_tag="PK264_17590"
     CDS             <6563..7596
                     /locus_tag="PK264_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HRD77722.1"
                     /translation="VEWQFRVAAGEALPLKQSAIKLSGHAIEARLCTEDPVRDFRPSA
                     GRIALFEPASGEALRIETGIETGSEVPPFYDSMVAKLITSGPDRETARARLVSGLRET
                     VLAGPQTNARFLTSLLEDDGVKAGRMDTGLIGREIARLTAAPELRPVAELGALHLVEA
                     ARDAVLAARAPHVGGWRSPWDAGDGFQLGSERRQTVKVLADGEPLTFDLWWRDEGPVV
                     PVMASNRPEDTGTLANAHVIEDGDRVLIIADMLQTEVAWPIYDPSSVEDEGGGTLRAP
                     INGRVAKMYVTPGATIAKGDRVAVVEAMKMEHVVTAPRDGTIASVGAKEGDQVVQGTP
                     LATLAEAQS"
     gene            7593..8030
                     /locus_tag="PK264_17595"
     CDS             7593..8030
                     /locus_tag="PK264_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HRD77723.1"
                     /translation="MTVHLVKLAVGCDGLDDLAAWQTERLAEQKRRGEAPRLFHRTFQ
                     TPKRRDDLLDGGSLYWVIKGIIQCRQRVIGLEEGAKDDGSPCCLVMLDPKLVAVRPVP
                     RRAFQGWRYMDAADAPKDIGRNETSIASLPPEMAKTLADLGLL"
     assembly_gap    8044..8226
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8227..>8469)
                     /locus_tag="PK264_17600"
     CDS             complement(<8227..>8469)
                     /locus_tag="PK264_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77724.1"
                     /translation="WLIGLVVLGGLAYYFLGQKLRAPSVPTTTAIMHNGVNISGQLNT
                     AIDGFKTTLGSIKDEATARAAVPGLQTAAKGIETVAD"
BASE COUNT         1203 a         2655 c         2772 g         1149 t
ORIGIN      
        1 cgccgtgcca cgtccgctcg gcgcaggcct cctcctccag ccgacggggc tcgcggcgct
       61 cgaccgcctc gggctcgccg gcaccgcgct cgtgcgcggc cagacgatcc gcggcatcga
      121 cggcaagacg gtgctcggcc gcggcgtgct cgccatcgac tacgccgatc tcgatccgcg
      181 cctctatggg gtcggcattc accgtgcggc cctcttccag atcctctacg acgcgggcca
      241 ggcagagcgg atcgcgctca tcgcggactg cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnngttccg gttcggtgcc gtctgggcgc cggtgccgct cccgcctgat tggccggcgc
      481 agaaccggct cgtgcagcgc tatcgcggcg cgcgcatcat gatcggcgtg ttgccgatcg
      541 gcacgctggc ggtcggtgat gcgccgttgg ccgcgctctt ctggagcttg ccggcggaca
      601 gctatcaggc ctggcgtcac cgcggcatcg acccgtggcg ggaggaggtc ggtgcgctct
      661 ggccagatgt cgcgccgctc atcgccaagc tcggctcggt cgacgacctc gccttcgcgc
      721 gctactcgga tctcacgacc gcgacgccca ttgcagaccg cctcgccatc atcggcgatg
      781 cggcgcattc gacgagcccg cagctcggcc agggggccaa tctcgcgctg atggacgccg
      841 aagcactctc gagcgcaatc gcggcacatc cgaacgcgct cgaggtgtca ctgcagacgt
      901 atgccgcctc ccgccgccgg cacgtgcgct tctaccagtt cgcgagccgc tggctgacgc
      961 cattcttcca gtcggagagc cggattgccg gtgccgtgcg cgacctgaca ttcggtcccg
     1021 cggcgaggct cggctatgtc cgccgtcaga tggtggcaac tctcgccgga ttgaagccgg
     1081 gcctcgttgc gctgccggac ctctcaggcg agctcagcct gccgccggcg ccatgaggtg
     1141 gatgccgtcg gcgagcagcg atactgtgat ctccgcgccc cgctcgaggc cgttgcggcg
     1201 tgcggcatgc gtcggcaggc ggaaattcag gaccgcgccc ggaaccgcct cgaccgtcat
     1261 ctcgaccgag gcctgctcgc cgaggatcac caggctgcgg atggtgccga cgaccgggtt
     1321 ctcgcgctcg ccgagcgacg gtcgcccccg ccggtgcagg acgacgtgat cgcgcgggat
     1381 cagccagtcc acggtttcgc cctcgcgaac gctgcctgtc gccgccacct cgagagcgat
     1441 gcccccccag tcgagccgac ctgttgcggg cgctagcgcc gacgtcgtca gccggcccga
     1501 gaagatgttg gtctgcccga gcagccgagc gaccgtcgag ctcgctgggc gcagccgcac
     1561 ctcctccggc gtgccgacct ggagcgtttt tccgcgctgg agcacggcaa tgcgatcggc
     1621 gagcatcatt gcctcgtcga gatcgtgggt gacgaggacg atagggatcg cgagacggtg
     1681 ccggatgagc gccagctcgc gctggaggcg ctggcgcgtc atctgatcga cggcggaaaa
     1741 gggctcgtcg aggagcagca cggcgggatc gcgggcgagt gcgcgcgcca gagctacgcg
     1801 ctgctgctgg ccgccggaca gcgccgccgg catgcgcctc tcgaggcccg cgagattgac
     1861 gaggtcgagg agtgcgcgcg cccttccttc acgctctcca gatggttggt caagcatggc
     1921 gagcatgacg ttgccgagcg cgtcgcggtg cggccagagc ccgtagtcct ggaagaccag
     1981 gccgacgcgg cgttgctgcg gcgagagcgc aaggctcagc gaggtatcga gccagacact
     2041 ctcgcccgag acgatccgtc cggacgccgg tgtcatgagc ccgctgatcg cccggaggat
     2101 cgtcgtcttg ccgctgcccg aggggccgac cagcgccagg agctcgcccg gcgcgcagtc
     2161 gaaggcgaca tccagcggga tcggcgcgtc ctggtcgagg cgcacgtgga gcgtcatggc
     2221 cggccccgtc gagggtcggt ccgccgtgcg gtccagttga cgagcgcaag agcaacgagc
     2281 gacgtgatca ccagcacccc ggcgatcact gcagcctcgg cattgcggaa tgcctgcacg
     2341 cgatcgtaga gcgcgatcga gagcgtctgc gtctcgcccg ggatgctgcc gccgatcatc
     2401 aggatgacgc cgaactcgcc gagcgtgtgg gcgacaacca gcgcgatcgc cgtgacgatc
     2461 ccgggccaga cgagcggcag ctcgatgcgc cagaaagttt gcagcggaga gaggccgctc
     2521 acagccgccg cctcgccaag actctgcggc aggctctcaa aggccctgac gatcggctgc
     2581 accgcaaacg ggatgttgac gatcagtgag gcgatcagga tgccggtgaa ggtgaacacg
     2641 agcggcccgc cggcaaggct cgctatgagc cgaccgaagc ccgagcttgg cgctaaggcc
     2701 atcagcaggc catagccgag cacggtcggc ggcaagacga gcggcagcag cagcaaggcc
     2761 tcgatgattg ccttgccggt gccgcgccgc cgcgcgagcg ctcggccgat cacaacgcca
     2821 gtgggaacga tcagcagcgc cgtccatagc gcgagggaga gcgagaggcg gaacgccgtc
     2881 cagtccatgg gcagatcaag ctccccgccc gaagccgaag cgatcgagaa cctggcgcgc
     2941 cggctcgctg tcgatatagg ccgcgaaggc gcgggccggt gcgcgggctc gggcgagcgt
     3001 cacggccgtc tgccggagcg gtgccgcgag gccatcgtcg acgaccgccg aactgacgcg
     3061 gccctcgagc atcggcgagc ggacgaggga gagcgcgacg agccccgctt cggcagcacc
     3121 cgatacgacg aactgggcgg cctgcgaaac cgtgtcgccg aggaccagcc gtttctgggc
     3181 ggcctcccag acgcctgccg ccatcagcgc ggctttcgcc cgctcgccgt agggagcatg
     3241 ctctggattg gcgatggcga agcggtggat ggcgcgggta tcgagcgcct ctgcgagccc
     3301 cgccagtcgc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgatgcgg
     3421 tcgtcggcgg cgagaaacag ctcgaacggt gccccctgct cgatctgtcg cgcgagattg
     3481 ccggtcgagc cgtaggtgat gcgcagattg atcggcggcg cgcctgacga gcggcgcact
     3541 gcgacatgag ccgccgcgat ctcgtcgagc gcatagcgca ggtccgaggc cgccgcgatc
     3601 agcgcatcat cggcggcgcg ggaggggccg ggcacgaggc acggcgcggc gaccgtggca
     3661 gcgacgccgc cgatgagcgc gcgccgggaa gcgatcatcg ccgcaccagg tcccagaact
     3721 cgccgcggaa gtagagaagc gggtcgatgg tctcgtcggc agtgccggcg acaacgcggc
     3781 cgatgaacac ggagtgcgtc gacatatcgc ggaactcggt cacctcgcag tcgaggctgg
     3841 cgatggcgcc cttcaggatc ggcgcacccg tgacgagcgt ggtccaggcg tcggcgtcga
     3901 agcgatcctc gccctcgacg cccgcgcgcc ccgagaagcg gagcgccaga tcggtctgcg
     3961 gccgggcaag catgttcacc gtgaagctct tctcggccat gatcggcgcg tgcgcgctcg
     4021 ccttccggtt gatgcacacg aggatcgtcg gcggggcgtc cgtcaccgag cagaccgccg
     4081 tcgcggtgag gccagtgcga tggcccggct tgccgacggc gatgatcgtc accgcgcccg
     4141 cctgatggcg catgatcgcc cgataggcat gcgcctccat caccgggctt tgattggact
     4201 ttgattggcc acccacgctt tcgtctccat cttgtggtgt gagtatgcca tgggttgtcg
     4261 gtcgccgtca gctgcgccgg ctgcggcgcg agcaacagcg tgattggccg cttgtcgaag
     4321 gcggcaagtc gtaggccgca tgcagcacgg cgccggcgga acgatgagga tcgagccttg
     4381 atgcgtggac tagcaggact tggagccatg ctcgtggcgc tcggcctcgc ctccccggct
     4441 gcggcagagg tcgcgacgcc ctgggccgat ggccacaaga cgcgggggcg cctcgtcgcg
     4501 ggcacggttc cggcgcctgg tggcccgcgc atcctggccg ggctcgagat cgagctcgcc
     4561 gacggctgga aaacctactg gcggacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnccgatc gagatcgcgc ccgaggcgcc gggcaagccc gccgagatca
     4801 aggtcgtggt cgagctcggc gtttgcaagg acatctgcgt gcccgtgacg ttcaatttcg
     4861 agctgacggt gccggatgcg cgcgcgctgc cggcgagcgt ccaccttgcc aaggccgtcg
     4921 acgccgtgcc gcgccgggcc gcagggcgcc gcgacgatga cccgatcatc gagcggcagg
     4981 tcgcggtgct ggatggagca aagccgagtc tgaccttcca cgtggcctac cccgcggggg
     5041 ccgtcggcgc cgacctcttc gtagaggccc ccgatggcgc ctgggtgccg ctccctgcga
     5101 tcaagcccga caaggacggc cgccgcgtca ccttcgagat cgacctctcg accggcgtcg
     5161 acctgaagga cctgatcggc aagccgctcg ccgtcaccgt ggtcgggcca acgggggcgc
     5221 tcgagacgag cacggtcctg aaggcgaaat agcgcccgtc gctgtcatcc cggccaagtg
     5281 cgaccccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnncagcgct cgatattgcg ggtggaggag atacggcagc ttcgccgctc ctgccgctgg
     5401 gtcccggatc gcctccgcgg cagaattgcc gctccggcgt ccgggatgac acggtgttgg
     5461 gtgtggtggg gcggagcgct catccctcct cgcgttttcg cgatcgggcc ggggaagcag
     5521 aacggtgact tgagcgaccc tgaatagtcc ccaggccgtg acttccgctg gcgcgcgcga
     5581 ttggctacat gacacgatca gctccgtcac cgggctcggg gagtcgcgca tgctttcgaa
     5641 ggtcctgatc gccaaccgcg gcgagatcgc gctgcgtatc atgcggacgg ccaagcggct
     5701 cggcatggcg accgtggcgg tctattccga ggctgaccgg gatgcgctgt tcgtgcgcca
     5761 tgcggacgag gccgtggcga tcggccccgc gccagcgacg gaaagctacc tccgcctcga
     5821 tcgcatcatc gaggccgcgc agaagaccgg cgccgactgc atccacccgg gctacggctt
     5881 cctctccgag aacgccgact tcccggatgc ctgcgcgaag gccggaatcg ccttcgtcgg
     5941 cccgtcgggc gaggccatgc gcaagctcgg cggcaaggcc gcggcgaaag cgatcgcgcg
     6001 ggctgccggc gtgacggtcg tgcccggcta tgatggcgat gcgcaggatg cgaagacgct
     6061 cgccgagcac gcaggccgca tcggctatcc cgtgatgatc aaggccgtgg cgggcggcgg
     6121 cggacgcggc atgcggctcg tcaccaagcc cgccgagttc gcagcagccc tcgaaagcgc
     6181 ggcgcgtgag gcggccgcct cgttcggcaa cccgcgcgtg ctcgtcgaga aggtcgttgt
     6241 cgcgccgcgc cacgtcgagg tgcaggtgtt cggcgaccgg cacggcaacg tcgtccacct
     6301 gttcgagcgc gactgctcgc tgcagcggcg caaccagaag gtgatcgagg aggcacccgc
     6361 gcctggcatg agcgagggct tgcgcagcgc gatcacggga gcggccgtga agctcgccaa
     6421 ggcggtcggc tacgagggtg ccggcacggt cgagttcctg gtcgagggcg gcacgctctc
     6481 cgccacggcg ccctggtact tcatcgagat gaacacgcgc ctccaggtcn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nntcgtcgaa tggcagttcc gcgtcgccgc cggcgaggca
     6601 ctgcccctga agcagagcgc gatcaagctc tcgggccacg ccatcgaggc gcgactgtgc
     6661 accgaggacc cggtgcgcga tttccggccg agtgccgggc gcatcgcgct cttcgagccg
     6721 gcgtcgggcg aggccttgcg catcgagacc ggcatcgaga cggggagcga ggtgccgccg
     6781 ttctacgatt cgatggtggc gaagctgatc acgtcgggac cggatcgcga gacggcgcgg
     6841 gcgcgactcg tcagtggcct ccgcgagacg gtgctcgctg gaccgcaaac caacgcgcgc
     6901 tttctcacga gcctgctcga ggacgacggc gtcaaggccg gccgcatgga tacgggcctc
     6961 atcggccgcg agatcgctcg cctcaccgcc gcacccgagc tgcgtccggt ggccgagctc
     7021 ggcgcactgc atcttgtcga ggccgcccgc gatgctgtgc tcgccgcgcg cgcgccccat
     7081 gtcggcggct ggcgttcgcc ctgggatgcc ggcgacgggt ttcagctcgg ctccgagcgg
     7141 cgccagacgg tgaaggtgct cgccgacggc gagccgctga ccttcgacct ctggtggcga
     7201 gacgagggac cggtcgtgcc ggtgatggcg tcgaaccgtc cggaggatac gggcacactc
     7261 gccaacgcac acgtcatcga ggacggcgat cgcgtgctga tcatcgcgga catgctgcaa
     7321 acggaggttg cctggccgat ctacgatccg agctcggtcg aggacgaggg tggggggacg
     7381 ctccgcgcgc caatcaacgg tcgcgtcgcg aaaatgtacg tcacccccgg cgcgacgatc
     7441 gccaaggggg accgcgtcgc ggtggtcgaa gcgatgaaga tggagcacgt ggtcacggcg
     7501 ccgcgcgacg gcacgatcgc ctcggtcggc gccaaggagg gcgatcaggt cgtgcagggc
     7561 acacccctcg ccaccctcgc cgaggcccag tcgtgacggt gcacctcgtc aagctcgcgg
     7621 tcggctgcga cgggctcgac gatctcgccg cctggcagac ggagcggctc gccgagcaga
     7681 agcgccgcgg cgaggcgccc cggcttttcc atcgcacctt ccagacgccg aagcggcgcg
     7741 acgacttgct cgacggcggc tcgctctact gggtgatcaa gggcataatc cagtgccgcc
     7801 agcgcgtgat cggcctcgag gaaggcgcca aggacgacgg ctcgccctgc tgcctcgtga
     7861 tgctcgatcc gaagctcgtc gccgtccgtc cggtgccgcg ccgcgccttc cagggctggc
     7921 gctacatgga tgcggccgac gccccgaagg acatcggccg caacgagacc tcgattgcga
     7981 gtctgccgcc cgagatggcc aagacacttg ccgatctcgg gctgctttga tcagagcctc
     8041 gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnngtcg gcgacggtct cgatgccttt ggcagcagtc tgcaggccgg gaacggcggc
     8281 acgggccgtc gcctcgtcct tgatcgagcc gagcgtggtc ttgaagccgt cgatcgccgt
     8341 gttgagctgg cccgagatgt tcacgccgtt atgcatgatg gccgtcgtcg tcggcacgct
     8401 cggcgcgcgc agcttctggc ctaggaagta gtaggcgaga ccgccgagca cgacgaggcc
     8461 gatgagcca
//
LOCUS       DAORNH010000200         8458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6196_length_8458_cov_2.682018, whole genome shotgun sequence.
ACCESSION   DAORNH010000200 DAORNH010000000
VERSION     DAORNH010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_8458_cov_2.682018"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="PK264_17605"
     CDS             complement(<1..1516)
                     /locus_tag="PK264_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRD77725.1"
                     /translation="MGQSAGTSGRSSRCIAIVGPYLSGKTTLLEAILARTGAVTRQGR
                     IADANTIGDSAPEARQHGMSVELNVAETRFLDDTFTFIDCPGSIEFQYEGLPALLACD
                     AAIVVCEPDPKRVPALQVILKGLQDRGIPHFLFLNKIDQFDARVRDTLTELQPASQRP
                     LVLRQVPIWENGIATGYVDLATERAYVYREHAPSELIEMPSAIKARETEARFQMLEKL
                     ADYDDELMEQLLSDMQPPRDRVFDDLTRELREGLICPVFLGSAEKGNGIFRLLKALRH
                     ECPFVETTARRLKLENAQSAAYIVKTAYSAHGGKLSIARVLAGEVGDGTILTGPKVEE
                     RVAGTFSLVGAEPKKRGAAKAGDTVALGRLEKTLTGETLTSGKSGINQIETPPRPAPV
                     LGLAISLPDRKDEVKLSSALSRLVEEDPTVIVDHATDTHQLVLWGQGEMHLRVALERL
                     KRKYAIEAKTAKRRLPYKETIRKTIEIRGRHKKQSGGHGQFGDVVLRIAPQPRGA"
     gene            1924..2913
                     /locus_tag="PK264_17610"
     CDS             1924..2913
                     /locus_tag="PK264_17610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD77726.1"
                     /translation="MVADRERVQSRVVSGLKTLSLALAGALALSGCAQLGDLPGLSSA
                     PAAPQPAATADATSQTELEKALDYWAKAYAKNPKDADAGLSYAKNLKAAGRKSQALAV
                     LQQLAMYHGSNRAVLSEYGRLALESDQITTALKVLEQADDPAHPDWRVISARGTAMAK
                     LGKYKEAIPLFERALTLSGNQPSILNNLALAYTMDGQPDKAEAMLRDAAAAPNADPRI
                     RQNLALVLAVQGKHAEARAVAGQDLAPEHADANMALLRKIVKAEGPAAPAAAPALAGA
                     PAATAVPAAFVTDPAPAAPTAPKAAAKPKPKSAAPSAATAAPAGSPTLRTSMD"
     gene            complement(2974..>3327)
                     /locus_tag="PK264_17615"
     CDS             complement(2974..>3327)
                     /locus_tag="PK264_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD77727.1"
                     /translation="PAAPADPKRLLGITSGNLEIAGRRIYRSQRADRFAGLDGMRCPG
                     VDYPKVLTGKAHFTVYGRCLPWDHLPGIMLLREAGWVDRHLDGSTYDVADLVGGVMSA
                     PGRDLLDEIVRRLTT"
     assembly_gap    3328..3373
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3374..3792)
                     /locus_tag="PK264_17620"
     CDS             complement(<3374..3792)
                     /locus_tag="PK264_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRD77728.1"
                     /translation="MLKPDIDRIGAILTEVAGELILPRWRNLSPSEITTKSGPRDLVT
                     VADLETEAALERRITSLYPDSTLVGEETFAADPTCLDRLKHGEPVWITDPIDGTGAFT
                     RGEDGFASMLVLVEQRRPVAAWIYQPVSGHLYCGETGG"
     gene            3791..>3981
                     /locus_tag="PK264_17625"
     CDS             3791..>3981
                     /locus_tag="PK264_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77729.1"
                     /translation="MPRLRDKAAGLSEMPLCCQIKATPDRSGAMPLRRRLLAEAQRLD
                     VFGDGLKGGAQRGAVARII"
     assembly_gap    3982..4069
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4070..5149)
                     /locus_tag="PK264_17630"
     CDS             complement(<4070..5149)
                     /locus_tag="PK264_17630"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD77730.1"
                     /translation="MTIHLWQARVTQLGRDKRGTVAILFAASVIPILLCVGLAIDGAR
                     GYYTASKTSAVLDAAALAAAKAMSEREISDAEVRDIAIATVKAHMAAETPRGITAASP
                     VVTIDRENDTVSVALTSHVDTFFAGLAGFPKWEVNKAASAVYGQRDIELGLMLDVSGS
                     MEDFGKIDDLKVAAADLVKALIPEAAEKRSVKIGLAPYSWSVNAGDYAERVRDDDSSG
                     PGGGKGKGHEKCVSERKGGSAFTDDSPGKGKKLGGKPSACPVNEILAISSSRSELLEK
                     LAGLSAAGSTAGHLGIAWAWYLVSPNWADIWPSESQPKPYGDRKVMKVVILMTDGMFN
                     TRYEADNGDSAEQARRLCDNIKATGI"
     gene            complement(5484..6677)
                     /locus_tag="PK264_17635"
     CDS             complement(5484..6677)
                     /locus_tag="PK264_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD77731.1"
                     /translation="MCLGQVANLVPHVVVPAVMPEHLIPLWRLSGTEAGLMASAYAVG
                     YMLAVPVLATLTDRVDSRLILIWGSLASAAATVAFGLLAGGLGSASIIWGLAGAGFAG
                     AYMPGLKALTDRLDPGDSSRAVTLYTSCFSLGVGLSFLVSQLIAKQYGWRAAFLVTGL
                     LPLLMVLVGLLLEPRPPPRRGGALLDFRPVVRNRPAMGYVLGYGAHCFELYGIRTWIV
                     AFWAFVAARQGGGALLEPITVSVLFTLLAMPSSILGNEAALMFGRHRAITVVMLASAT
                     VAILLALSTSLPAAVLLMLVVAYAITVPADSGALTSGMAIAAAPEHRGATMALHSTAG
                     FGLSALGALAFGVALDLGGGPMTERGWTLALLLNAAAVLLGPLALMWSRRVHSAVPSR
                     DDGRA"
     gene            6907..7365
                     /locus_tag="PK264_17640"
     CDS             6907..7365
                     /locus_tag="PK264_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HRD77732.1"
                     /translation="MTDLPFWTAKPLEALSADEWEQLCDGCGRCCLLKLEDDDTGAIH
                     LTRLACRLLDISTCRCSDYENRHRRVADCLSITPRSVRELAWLPETCGYRRVAEGREL
                     AWWHPLVSGSTETVHEAGISVRTLARSETGVRPASLFKYIIDGYPAAGPR"
     gene            complement(7341..7844)
                     /locus_tag="PK264_17645"
     CDS             complement(7341..7844)
                     /locus_tag="PK264_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HRD77733.1"
                     /translation="MEPTPPPRDADTSTLVRFVLTPNRSLPPVGFVLLMASLITVSFV
                     TGTMFLAIGAWPVPMFLGLDVAIVYLAFRLNYRSGRRAEIIEIDGAELRLIRCTPSGK
                     TDRLVLNPYWVRVRLDEARDGGNRIALSSHGRSYAFAGFLNDDERRELAPALEAALVR
                     QRGPAAG"
     assembly_gap    7981..7995
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7996..>8458
                     /gene="nth"
                     /locus_tag="PK264_17650"
     CDS             <7996..>8458
                     /gene="nth"
                     /locus_tag="PK264_17650"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRD77734.1"
                     /translation="SRAGGGAKAKAPPRKASRPKVKRQVRPDASRVGAIFGRLAAAMP
                     EPKGELEHVNVFTLLVAVVLSAQATDAGVNKATRALFQVADTPARMLALGIDALTEHI
                     KTIGLFRMKAKNVIALSEQLIRDHAGEVPRDRGALEALPGVGRKTANVVLN"
BASE COUNT         1413 a         2699 c         2848 g         1349 t
ORIGIN      
        1 cggcgccacg cggttgcggc gcaatgcgca agacgacatc gccgaactgc ccgtgcccgc
       61 ccgactgctt cttgtgccgg ccgcggattt cgatcgtctt gcggatggtc tctttgtaag
      121 gcagccggcg ctttgccgtc ttcgcctcga tggcgtactt gcgcttgagc cgctccaggg
      181 cgacgcgcag gtgcatctcg ccctgccccc aaagcacgag ttggtgtgtg tccgtcgcgt
      241 ggtcgacgat gacggtcggg tcctcctcga cgagccgcga gagggcggaa gagagcttca
      301 cctcgtcctt gcggtcggga agcgagatgg cgaggccgag cacgggcgca ggccggggcg
      361 gtgtctcgat ctggttgatg ccgctcttgc cgctcgtcag cgtctcgccg gtcagcgtct
      421 tctctagccg gccgagcgcc accgtgtcgc cggccttggc cgcgccacgc ttcttcggct
      481 cggccccgac gagcgagaac gtgccggcga cccgctcctc gaccttggga cctgtcagga
      541 tcgtaccgtc gccgacctcg ccggcgagca cgcgcgcgat cgacaacttg ccgccgtggg
      601 cggaataggc ggtcttcacg atgtaggccg cggattgtgc attctcgagt ttcaggcgac
      661 gtgccgtcgt ctcgacgaac ggacactcgt gacggagtgc cttcaggaga cggaagatgc
      721 cgttgccctt ctcggcactg ccgagaaaca cggggcagat gaggccttcg cggagctcgc
      781 gcgtcagatc gtcgaacact cggtcgcgcg gcggctgcat gtcggagagc agctgctcca
      841 tgagctcgtc gtcgtagtcg gcgagcttct cgagcatctg aaagcgggct tccgtctcgc
      901 gcgccttgat tgccgatggc atctcgatca gctcggaggg cgcatgctcg cgatagacgt
      961 aggcgcgctc ggtcgcgaga tcgacgtagc cggtggcgat gccgttctcc cagatcggca
     1021 cctggcgcag caccagcggc cgctggctcg ccggctgcag ctcggtcagt gtgtcgcgga
     1081 cgcgcgcgtc gaactggtcg atcttgttca ggaacaggaa atgcgggatg ccgcgatcct
     1141 gcaagccctt cagaatcact tgcagcgcgg ggacgcgctt cgggtcgggc tcgcagacga
     1201 cgatggcggc gtcgcaggcg aggagggccg gcagaccctc gtactggaac tcgatggagc
     1261 cggggcagtc gatgaatgtg aacgtgtcgt cgagaaagcg cgtttcggcg acgttgagct
     1321 cgacactcat gccgtgctgg cgggcctcgg gggcactgtc accgatcgtg ttggcgtcgg
     1381 cgattcggcc ttggcgcgtc acggcgccgg tgcgggcgag gatggcctcg agcaaggtgg
     1441 tcttgcccga aaggtagggc ccgacgattg cgatgcagcg ggaactgcgg ccgctggtgc
     1501 cggcggattg ccccatggtg tcctccatct tgctcaggca aagcgggacg accggcgcct
     1561 cgcgcctgag tggacagggc gcgtcgaccc acatggtgga cctcgcgacc ggcgagcgca
     1621 agggcctctc cgcagggcct ccgctgtcat gtccgatctc tctgccgcac gtccggcaat
     1681 cactccggcg cgcgttgcac ccgaacctgg tcgcacgtcg ctccggggca gaacggcgca
     1741 ccaatgagga tgcaaccagg atcgccaaga cggccggcga tacgctcagg ccgcgcgccc
     1801 atcgctgatg gcgtgatcga tcgccctgtc ccatctcgaa acggcgatta agcctttgtt
     1861 gagggctctc cgccacactc cacctaaatc gatcaagcgc ctttatgcgc cgaggagtgg
     1921 acgatggtcg ccgaccgcga acgagttcag agccgcgtag tgtctggcct caaaaccctt
     1981 tccctggcgc tcgctggcgc actcgcgctc agtggctgtg cccagctcgg cgatctgccc
     2041 ggcctgtcgt cggctccggc agcgccgcag cccgccgcga cggccgatgc gacctcccag
     2101 accgagctcg agaaggcact cgactactgg gccaaggcct atgccaagaa cccgaaggac
     2161 gccgacgccg gcctctccta cgccaagaac ctgaaggccg cgggccgcaa atcacaggcg
     2221 ctcgccgtgc tccagcaact cgcgatgtat cacggctcaa accgcgcggt gctgtccgag
     2281 tacggccgcc tggcgctcga atcggaccag attaccacgg ccctcaaggt gctcgagcag
     2341 gccgacgacc cggcgcatcc cgactggcgc gtgatcagcg cccgcggcac ggccatggcg
     2401 aagctcggca agtacaagga ggcgatcccg ctcttcgagc gcgcgttgac gctttccggc
     2461 aaccagccgt cgatcctcaa caacctggcg ctcgcctata cgatggatgg ccagcccgac
     2521 aaggccgagg cgatgctgcg cgatgcggca gccgccccga atgccgatcc gaggatccgc
     2581 cagaacctgg cactcgtgct cgctgtccag gggaagcacg ccgaggcgcg ggccgtcgcc
     2641 gggcaggacc tcgcgcctga gcacgcggac gccaacatgg cgctgctgcg caagatcgtg
     2701 aaggccgaag ggcctgcggc gcctgctgct gcaccagctc tcgctggcgc accggctgca
     2761 acggcagtgc cggcagcttt cgtgaccgat ccggctccag ctgcgccgac ggctcccaag
     2821 gccgcggcaa agcctaagcc caagtccgcg gcaccctctg ctgcaaccgc ggcaccggcc
     2881 ggatcgccga cgctgcgcac gtcgatggat tgagcaacat cgatcgcaga tcgcagacat
     2941 ttggtggggc tccgcggatg cggggcctca aatctatgtg gtgaggcgtc tgacaatttc
     3001 gtcgagcaag tcgcggcccg gcgcgctcat gacgccgccg acgagatcgg cgacatcgta
     3061 ggtgctgccg tcgaggtgtc ggtccaccca gccggcctct cggagcagca tgatgccggg
     3121 cagatggtcc cacggcaggc agcggccgta gaccgtgaaa tgcgccttgc ccgtcaacac
     3181 cttcggatag tcgacacccg ggcaccgcat gccgtcgagg ccggcgaagc ggtcggcgcg
     3241 ctggctgcga tagatgcgtc gaccggcgat ctcgagattg ccgctggtga tgccgaggag
     3301 gcgcttcgga tcggcggggg cggcgggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnccgcctg tctcgccaca gtagaggtgc ccgctcaccg gctggtagat
     3421 ccaggcggcg accggccggc gctgctcgac gagaaccagc atcgaggcaa agccgtcctc
     3481 gccccgcgtg aaggcgccgg tgccgtcgat cgggtcggtg atccagacgg gctcgccatg
     3541 cttcaggcgg tcgaggcaag tcggatccgc cgcaaaggtc tcctcgccga cgagcgtcga
     3601 gtccggatag agcgaggtga tgcgccgctc gagtgctgct tccgtctcga gatcggcgac
     3661 cgtcacgaga tctcgtgggc ccgatttcgt ggtgatctcg ctgggcgaga ggttgcgcca
     3721 gcgcggcagg atcaactccc cggcgacttc ggtgaggatc gcaccgatgc ggtcgatatc
     3781 aggcttcagc atgccgcggc tccgggacaa ggcggcaggc ttatccgaga tgccgttatg
     3841 ctgccagatc aaagcaactc ccgatcgctc cggcgcaatg ccattgcgcc gtcgactact
     3901 tgctgaggcg cagcgacttg atgtgttcgg cgatggtctg aaaggcggtg cgcagcgagg
     3961 cgccgtcgcg cgcatcatag annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atgccggtcg
     4081 ccttgatgtt gtcgcagagg cggcgcgctt gctcggccga gtctccgtta tcggcttcgt
     4141 agcgcgtgtt gaacattccg tccgtcatga ggatcaccac cttcatgacc ttgcggtccc
     4201 cgtagggttt cggttggctc tcactcggcc agatgtcggc ccagttgggc gagacgagat
     4261 accaggccca ggcgatgccg agatgacccg ccgtggagcc tgcagccgag agcccggcga
     4321 gcttttcgag cagttcgctg cggctcgagc tgatggccag gatttcgttc accgggcacg
     4381 cgctcggctt gccgccgagc ttcttgccct tgccggggga atcgtcggtg aacgccgagc
     4441 cgcccttgcg ctcacttacg cacttctcat gacccttgcc cttgcccccg cccgggccgc
     4501 tgctgtcgtc gtcgcgcacg cgctccgcat agtctccggc attgacggac cacgagtaag
     4561 gcgcgaggcc gattttcacc gaacgtttct cggcggcttc gggaatgagc gctttgacga
     4621 gatcggcggc tgcgaccttg agatcgtcga tcttgccgaa atcctccatc gagccgctga
     4681 cgtcgagcat caggccgagt tcgatgtcgc gctgcccata aacggcggag gcggccttgt
     4741 tgacctccca cttgggaaag ccggcgagcc ccgcgaaaaa ggtgtccacg tgcgacgtta
     4801 gcgcgacgct caccgtgtcg ttctcgcgat ctatcgtgac aacgggggag gcggccgtta
     4861 tgccgcgcgg cgtctcggcc gccatgtggg ccttgacggt cgcgatggca atgtcacgta
     4921 cctcggcatc agagatctcg cgctcgctca tggccttggc cgcggcgagg gccgcggcat
     4981 cgaggactgc agaagtcttg gaggcggtgt agtagccgcg cgcgccatcg atggcgaggc
     5041 caacgcagag caggattggg atcacggatg cggcaaacag gatcgcgacc gtgccccgct
     5101 tgtccctgcc gagctgggtg acacgtgctt gccagaggtg gatggtcatg cggcgctcat
     5161 cgtttaccga ttcgaacttc gaccggtacg atgaggagta gcaaatgcct gttcagtaac
     5221 cgttgaaaat tgcagggaaa ttctgatcgt gcgcgtaagc agtcattaac gacgctacgc
     5281 gcagcccgcc gcaaagtccc agtcgaggag gcgccggatt cgccgcctgc tgcgcgatct
     5341 ccgcacccgg gaatgccctt gcgctcatgg ccgcggtcgt tgtctcgccc cgtgcgggca
     5401 tatggagccg cacgtgcccc cgttcactcc aggcgatggg aatgcgccct aacgtgatct
     5461 caatcatagc ctttctggca ctctcaggca cggccgtcgt ccctcgacgg gacggcggaa
     5521 tgaactctgc gcgaccacat gagcgccaag ggaccgagga gaactgcagc ggcattgagc
     5581 agaagggcca gcgtccagcc cctctcggtc atggggccgc cgccgaggtc gagcgccacg
     5641 ccaaaggcga gtgcgccgag agcggagagc ccgaaccctg cggtcgaatg cagcgccatg
     5701 gtcgcgccgc ggtgctcggg cgctgccgcg atcgccatgc ccgacgtgag cgcacccgag
     5761 tcggccggca ccgtgatcgc ataggcgacg accagcatga gcaggacagc cgccggcagc
     5821 gaagtcgaga gggccagcag gatcgcgacc gtcgccgagg caagcatgac gacggtgatg
     5881 gcacgatggc gcccgaacat cagtgccgcc tcgttgccga ggatggacga gggcatcgcg
     5941 agcagcgtga aaagaacgct caccgtgatc ggctcgagga gggcgccgcc gccctggcgt
     6001 gcggcaacga acgcccagaa cgcaacgatc caggtcctga tgccgtagag ttcgaagcag
     6061 tgtgcgccat agccgagcac gtagcccatc gccggtcgat tgcgcacgac gggtcggaaa
     6121 tcgagtagag cgccgccccg ccgcggtggc ggacgcggct cgagcagcag cccgaccagt
     6181 accatgagca gcggcaaaag gcctgtaacc aggaatgcgg cgcgccaccc gtactgcttg
     6241 gcgatcagct gcgagacgag aaacgagagg ccgacgccga gcgagaagca cgacgtatat
     6301 agtgtgaccg cgcgcgagct gtcgcccgga tcgaggcggt cggtgagggc cttcagccct
     6361 ggcatgtagg cccctgcaaa gcccgctccg gcgagccccc agatgatgga cgccgagccg
     6421 aggccgcccg caaggagtcc gaacgcgact gttgccgccg cgctcgcgag cgatccccag
     6481 atgaggatga ggcgcgagtc cacgcggtcg gtgagtgtcg cgagcacggg gaccgcgagc
     6541 atgtagccga cggcgtaggc gctcgccatc aggccggcct cggtgccgga gaggcgccag
     6601 agcgggatca ggtgttcggg catgacggcc gggacgacga catgcgggac gaggtttgcg
     6661 acctgcccga ggcacatggc gacaatcagt ccgcgtccac tgagcttcgg catcgtgcat
     6721 tcctggttga tgcgaaagcg tcgtcgctac agcagtcgcg gtcaaggccc tggcgcgcgt
     6781 ggcgcaccgt tcgatattga ttgggtccga tcgcgggtgt ggcctcagtg acggaactcg
     6841 actattttcg cccctggcga tgaccgacac ccgtgagccc agatggcaat cgagccctgt
     6901 aagactgtga ccgacctgcc cttctggacg gcgaagccgc tcgaggcctt gtcagcggac
     6961 gagtgggaac agctttgtga cggctgcggg cgatgctgcc tcctgaagct cgaggacgac
     7021 gacaccggcg ccatccatct gaccaggctt gcctgccggc ttctcgacat ctccacgtgc
     7081 cgctgctcgg actatgagaa ccgccaccgc cgcgtcgccg actgcctgtc gatcacgccg
     7141 cgttcggtgc gcgaacttgc gtggctgccg gagacctgcg gctacaggcg cgtcgcggaa
     7201 gggcgtgagc tcgcatggtg gcatccgctg gtctcgggct cgaccgagac cgtgcacgag
     7261 gcgggcatct cggtgcgcac tttggcgcgc agcgaaacgg gggtccggcc ggcctcgctc
     7321 ttcaagtaca tcatcgatgg ctatccggcg gccgggccgc gctgacggac gagggcagct
     7381 tccagcgctg gcgcgagctc gcggcgttcg tcgtcgttca ggaagccggc gaacgcgtag
     7441 gagcggccat gcgaggagag cgcgatgcgg tttcctccgt cgcgcgcttc atcgagtcga
     7501 acgcgcaccc agtagggatt gagcacgagc cggtcggtct tgcctgaggg agtacagcgg
     7561 atgagccgca gctcggcgcc gtcgatctcg atgatctcgg cgcgtctgcc ggagcgatag
     7621 tttaggcgga acgcgagata gacgatcgcg acatcgaggc cgagaaacat ggggaccggc
     7681 caggcgccga tggcgagaaa catagtgcca gtcacgaagc tgacagtgat gaggctcgcc
     7741 atcagcagca cgaaaccaac gggcggcagc gagcgattgg gcgtcagcac gaaccgcacg
     7801 agcgtactcg tgtcggcatc cctcggcggc ggggtcggct ccacggcgtc ctcgtcctct
     7861 gtccggcatt cgcgtagatg tgacacacta caggttgtcg tggccagggg gccggatggc
     7921 tcgtggcctc gaaacgaggg gatggcgagc ggccagaggg gacaggatgc caatgacggc
     7981 nnnnnnnnnn nnnnnggagc cgggcaggcg gcggcgcgaa ggcgaaagcc ccgccgcgca
     8041 aggcgtcccg gcccaaggtc aagcgtcagg tacgccctga tgcgtcccgc gtcggcgcga
     8101 tcttcggccg gctggcggcg gcaatgcccg agcccaaggg cgaactcgag catgtcaacg
     8161 tcttcacgct gctcgtcgcg gtggtgctct cggcgcaggc gaccgatgcc ggggtcaaca
     8221 aggcgacgcg cgcgctgttt caggtggccg acacgcccgc ccgcatgctg gcgctcggca
     8281 tcgacgccct gaccgagcac atcaagacga ttggcctctt ccgcatgaag gcgaagaacg
     8341 tgatcgcgct ctcagagcag ctgattcgcg atcacgccgg cgaggtgccg cgcgacaggg
     8401 gggcgctcga ggcgctgccg ggtgtcggcc gcaagaccgc gaacgtcgtg ctcaacat
//
LOCUS       DAORNH010000201         8425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6228_length_8425_cov_2.753405, whole genome shotgun sequence.
ACCESSION   DAORNH010000201 DAORNH010000000
VERSION     DAORNH010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6228_length_8425_cov_2.753405"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK264_17655"
     CDS             <1..601
                     /locus_tag="PK264_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HRD77735.1"
                     /translation="GVTAEDLVGAQCRAVVGLGNAKTAEWASGKATVGVWHGDAASAA
                     ARQAALTCVPDGKFEALAISPLASGRLDPPDIALFYATPGAMMYFINGMQYVGYRKFE
                     WGIVGETACADSWGRALAKREPSLSIPCFAERRYGGVLDDEMLMALPPDQLDKAISGM
                     EALAKNGLRYPFPQYGIQQDVRAGMAASYPDRAPPPAKS"
     gene            676..972
                     /locus_tag="PK264_17660"
     CDS             676..972
                     /locus_tag="PK264_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77736.1"
                     /translation="MYRLLLTSGNIVASLLLGAIAMVVFALYNENAFEQILNGAAQLK
                     SWMVSTGLPTKWNNFVRLFLDEKTIVFSGFTIATRIVLAILIELGRALFSSPAR"
     gene            1012..1614
                     /locus_tag="PK264_17665"
     CDS             1012..1614
                     /locus_tag="PK264_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD77737.1"
                     /translation="MTTVTSIDALRRLYDMPQERAVKKELTRLDRHHKSFIALSPFLV
                     ISSTGANGQVDASPRGDRAGFVQVLNDATITIPDWPGNNRLDTLTNIIERPQVGLLFL
                     IPGVTEQMRVNGRAVIDTEESLRRPFETRGKLPKSVIRVSVEQAYLHCSKAIMRSSLW
                     DESRKVDRSVLPTLTEMLRDQTGIASSEADLQARYQKTLY"
     gene            1646..>2452
                     /locus_tag="PK264_17670"
     CDS             1646..>2452
                     /locus_tag="PK264_17670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD77738.1"
                     /translation="MSELCDLSAVEARRLIGAKKLSPVELMASCLKRIESTNKPVNAV
                     VAMIDAREAKKAAKAAEQAVMRGDDLGLLHGLPIGVKDLQDTEGLRTTYGSLLYKDHV
                     PKADNTTIARIRSEGGIILCKTNTPEFGAGSNTTNRVYGPTGNPFNPELTCAGSSGGS
                     AVSLALGHVPLATGSDYGGSLRTPAGFCGVAGFRPSPGVVPGETRPMLLAPFPVSGPM
                     GRTLEDAHLLLMGQIDVDKGDPFSSGDTFHIPQRLGNLDLSTIRAAFSVDL"
     assembly_gap    2453..2609
                     /estimated_length=157
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2610..>2840
                     /locus_tag="PK264_17675"
     CDS             <2610..>2840
                     /locus_tag="PK264_17675"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD77739.1"
                     /translation="RERLEKHRDLLDRNVIDNTERGLKYSLGDVAWANVEQSKIYKGF
                     LKFFEDADVLIAPAASVSPFPHKQLFVEEINGD"
     assembly_gap    2841..2862
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2863..3074
                     /locus_tag="PK264_17680"
     CDS             <2863..3074
                     /locus_tag="PK264_17680"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRD77740.1"
                     /translation="ITYAPTMALATSAVIPCGLDHKGMPFGIQVMGPNGADGLVLEVA
                     HALEQVLASDPATQRPNPDLKKLGA"
     gene            3106..4029
                     /locus_tag="PK264_17685"
     CDS             3106..4029
                     /locus_tag="PK264_17685"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRD77741.1"
                     /translation="MTARSNQRVAYFNGKYVPEAEVRVPFRDRSFLYGDGCFDMTRTF
                     NGKPFRVEEHIERFYRSLSYLRIDIGLSAKEMVDITHEVVRRNAHLLGKGDDFWVGQR
                     VSRGVRAVGDEGYDHTGPNIVVDCQPLPFKTRAAHYRDGIRVIVPSVRRTPPSSLSPR
                     VKSHNYLNMVMGELEVKDRDPDAWAVLLDENGNLCEGIGSNIFTVKEGRISTPEGRYV
                     LEGISRQTVIELARKLGYEVVERGIDLYDAYTADEVFLTSTSLCICPVASVNGNRIAG
                     GKVPGAATKRILDAYSDMVGCDIQGQYLARL"
     gene            4095..4526
                     /locus_tag="PK264_17690"
     CDS             4095..4526
                     /locus_tag="PK264_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77742.1"
                     /translation="MRRYLAALVVAVATFALVGSSPAEAKKLCFGTDEHLRFVQKLDA
                     KGPNGEPLELARKIRMECFVAPYTVADDGYVLKIVGANRYFSLTPEIIAGMQKANQLP
                     NPMPPYQLDTMDRIFGHLLWILIGVFALVSLVSWLRKRSRA"
     gene            complement(4626..>5538)
                     /locus_tag="PK264_17695"
     CDS             complement(4626..>5538)
                     /locus_tag="PK264_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HRD77743.1"
                     /translation="YYHDGRIGTAIVVREPMVLGHEVAGRIEALGAGVTSVKVGDRVA
                     LNPSRPCGACRYCQAGLQMHCLDMRFYGSAMRFPHVQGAFRDAIVAEAGQCHVVPESL
                     SAGEAAMAEPLAVCLHAARRAGPLLGRKVIVTGSGPIGALCAIAARRAGASLIVATDI
                     SDAPRVTLEKLGVDRFVNVAKDKAALADYAKDKGTFDVAFEASGAAPAVAAAIDVLKP
                     CGIIVQVGLGGEMALPMNAIVAKEIEVRGTFRFHEEYGLATELLGKRLVDVRPLITAT
                     LPVEKAVEAFGLASDRGRSMKVQLSFA"
     assembly_gap    5539..5548
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5549..5697)
                     /locus_tag="PK264_17700"
                     /pseudo
     CDS             complement(5549..5697)
                     /locus_tag="PK264_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008529987.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
     gene            5832..6725
                     /gene="gluQRS"
                     /locus_tag="PK264_17705"
     CDS             5832..6725
                     /gene="gluQRS"
                     /locus_tag="PK264_17705"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRD77744.1"
                     /translation="MQRAEILRFAPSPTGALHLGSALSALTGFAIARHLGGRFLVRME
                     DTDQTRCRPELAAAILADLAWLGVSWEEPVLVQSQHYDVYRQAAARLEAMGLLYPCFA
                     SRTEILAAADPNARDPDGAPLYPRLHKGMAPVEVARRRARDEPVALRLDMDKALAAAT
                     RLLGGALLTFEALDEYGQPTTVTAQPERWGDVVIVGKERPTSYHLSVVVDDARQGVTL
                     VTRGLDLLAATDLHRLLQVLLNLPAPRYHHHRLVLAEDGKKLSKSARDTSIADLRAAG
                     ASPADIRRLIGPIDLSSWPRR"
     gene            complement(6703..>7024)
                     /locus_tag="PK264_17710"
     CDS             complement(6703..>7024)
                     /locus_tag="PK264_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HRD77745.1"
                     /translation="VSSPGDAERAAHYLDAGAADLLLFDAKPPPSAPLPGGNGVPFDW
                     RLLDAPGVRRPFMLAGGLTAENVRDAVRLVGPAFVDVSSGVETSPGVKDADLIRRFIA
                     AARS"
     assembly_gap    7025..7214
                     /estimated_length=190
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7215..7335)
                     /locus_tag="PK264_17715"
     CDS             complement(<7215..7335)
                     /locus_tag="PK264_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HRD77746.1"
                     /translation="MSIGIKICGIRTSDALSAAREAGADLFGLVFFPKSPRNIA"
     gene            7597..>8425
                     /locus_tag="PK264_17720"
     CDS             7597..>8425
                     /locus_tag="PK264_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77747.1"
                     /translation="MAEHGSTRSKRRHAGKLVRLIMAGTFAILGVSTVSVMVSAGADY
                     GFVRSTSSWIEKTSLLSWTGDNNAKQAITATGASALILLLAAFYVCGLGRWQSEFRYD
                     KRAHDLLYYLGFIGTLSGLGAAALTLVMTEKAGSPKVTMLVAQNGVALMTTLVGLLGR
                     NLLSYMFPPPEAVERTAAEIGRINAELQTLGTTVKGVSGNFSAFSDTVTGTRAAIEAH
                     HEPWERLRQQAEQTHAPLTAAAQSFGAVAGSVGALSGQLQGSLDAVGRLAASTGEVAT
                     "
BASE COUNT         1369 a         2755 c         2620 g         1302 t
ORIGIN      
        1 cggcgtcacg gccgaggacc tcgtcggcgc gcagtgccgg gcagtcgtcg gcctcggcaa
       61 tgccaagacg gcggagtggg cgagcggcaa ggcgaccgtc ggcgtctggc acggcgatgc
      121 agcctcggcc gcggcgcgac aagccgcgct cacctgcgtc cccgacggca agtttgaggc
      181 gctcgccatc tcgccgctcg cctcgggccg gctcgatccg cccgatatcg cgctcttcta
      241 tgcgaccccc ggcgccatga tgtacttcat caacggcatg cagtacgtcg gctacaggaa
      301 gttcgagtgg ggcatcgtcg gcgagacggc ctgcgccgac agctggggac gtgcgctcgc
      361 caagcgcgag ccgtcgctgt cgatcccctg cttcgccgag cggcgctatg gcggcgtcct
      421 cgacgacgag atgctgatgg cgctgccgcc agatcagctc gacaaggcca tctcgggcat
      481 ggaagccctc gccaagaacg gcctgcgtta tcctttcccg caatacggca tccagcagga
      541 tgtgcgcgcc ggcatggccg cgagctaccc cgaccgcgca ccgccgccgg caaagtcatg
      601 acaaagcctt gtgctcaggg tagagactag ggcagctgcc ggatcggggc gccgagcgcc
      661 agggggaggg gacgcatgta ccgactgctg ctgacctcgg ggaacatcgt cgcctcgttg
      721 ctgctcggcg ccatcgccat ggtcgttttc gcgctctaca acgagaacgc cttcgagcag
      781 atcctgaacg gcgccgccca attgaaatcc tggatggtca gcaccggcct gccgaccaag
      841 tggaacaatt tcgtacgcct gttcctcgac gagaagacga tcgtcttctc cggcttcacg
      901 atcgcgacgc gcatcgttct tgcaatcctg atcgagctcg gacgcgcctt gttcagctcg
      961 ccggcccggt gatacggtcg gcgcttctcg cgacgagggg agcgccggcc catgacgacc
     1021 gtgacgagca ttgacgcgct gcgacgcctc tacgacatgc cgcaggagcg cgcggtcaag
     1081 aaggagctca cccgcctcga tcggcaccac aagagcttca tcgcgctgtc gccgttcctg
     1141 gtgatctcct cgaccggcgc gaacgggcag gtcgacgcct cgccccgcgg cgacagggcg
     1201 ggcttcgtcc aggtgctcaa cgatgcgacg atcacgatcc cggattggcc gggcaacaac
     1261 cgcctcgaca cgctgaccaa catcatcgag cggccgcagg tgggcctcct cttcctcatt
     1321 cccggcgtca ccgagcagat gcgcgtcaac ggccgcgccg tgatcgatac cgaggagagc
     1381 ctccggcgcc ccttcgagac gcgcggcaag ctgccgaagt ccgtgatccg cgtcagcgtc
     1441 gagcaggcct atctccactg ctccaaggca atcatgcgct cgagcttgtg ggatgagagt
     1501 cgtaaggtcg atcgctccgt gctgccgact ctgaccgaga tgctccgcga ccagaccggc
     1561 atcgccagca gcgaggccga tctccaggcg cgctaccaga aaacgctcta ctgatccctc
     1621 cgcctccaga caatcgagcc aagcaatgtc cgagctgtgt gatctctccg ccgtcgaggc
     1681 ccgccgcctg atcggcgcca agaagctctc acccgtcgag ctgatggcga gctgcctcaa
     1741 acgaatcgag tcgaccaaca agccggtcaa cgccgtcgtc gccatgatcg acgctcgcga
     1801 ggcgaagaag gccgccaagg ccgccgagca ggccgtgatg cggggcgacg atctgggtct
     1861 cctccatggc ctgccgatcg gtgtgaagga tttgcaggac accgaggggc tccggaccac
     1921 ctacggctcg ctcctctaca aggaccacgt accgaaggcc gacaacacga cgattgcccg
     1981 catccgctcc gagggcggca tcatcctctg caagaccaat acgcctgagt tcggtgcggg
     2041 ctccaacacg acgaaccgcg tctatggccc gaccggcaat ccattcaatc cggagctcac
     2101 gtgcgcgggg tcctccggcg gctcggcagt gtcgctcgcc ctcgggcatg tgccgctcgc
     2161 gacgggctcc gactacggcg gcagcctccg gacgcccgcg ggcttctgcg gcgttgcggg
     2221 gttccgaccc tcgcccggcg tcgtgcccgg cgagacgagg ccgatgctgc tcgcgccctt
     2281 cccggtttcg gggccgatgg gacgcacgct cgaggacgcg catctcctgc tgatgggcca
     2341 gatcgacgtc gacaagggcg accccttctc gagcggtgat acgtttcata tcccgcagcg
     2401 cctcggcaac ctcgatctct cgaccatccg cgcggccttc tcggtggatc tcnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gcgagcggct cgagaagcac cgcgatctcc
     2641 tcgaccgcaa tgtgatcgac aacacggagc gcggcctcaa gtattcgctt ggcgatgtcg
     2701 cctgggccaa tgtcgagcag agcaagatct acaaaggctt cctcaagttc ttcgaggacg
     2761 ccgatgtgct gattgcgccg gcagcctcgg tgtcgccgtt cccgcacaag cagctgttcg
     2821 tcgaggaaat caacggcgac nnnnnnnnnn nnnnnnnnnn nncgatcacc tacgcgccga
     2881 cgatggcact cgcgacatct gcggtgatcc cctgcggtct cgaccacaaa ggtatgccgt
     2941 tcggcattca ggtgatggga cccaatggcg ccgacggcct cgttctcgag gtcgcgcacg
     3001 cgctcgagca ggtgctggcg tccgatccgg cgacgcagcg gcccaaccca gacctgaaga
     3061 agctcggtgc ctgaaggccg agagcgacaa ttcggagcag cagccatgac cgcaagatcg
     3121 aaccagcgcg tcgcctactt caacggcaaa tacgtgcccg aagccgaggt gcgcgtcccc
     3181 ttccgcgacc gcagcttcct ctacggcgac ggttgcttcg acatgacccg caccttcaac
     3241 ggaaagccct tccgcgtcga ggagcacatc gagcgctttt accgctcgct ctcttacctg
     3301 cggatcgata tcggcctctc ggccaaggag atggtcgaca tcacgcacga ggtcgtgcgc
     3361 cgcaatgccc atctcctcgg caagggtgac gacttctggg tcggccagcg cgtctcgcgc
     3421 ggcgtgcgcg ccgtcggcga tgaaggctat gaccacaccg ggccgaacat cgtcgtcgac
     3481 tgccagccgc tcccgtttaa gacccgcgcg gcgcactacc gcgacggcat ccgcgtgatc
     3541 gttccttccg tgcgccgcac gccgccgtcc tcgctctcgc cccgcgtgaa gagccacaac
     3601 tacctcaaca tggtgatggg cgagctcgag gtgaaggatc gcgatcctga cgcctgggcc
     3661 gtgctcctcg acgagaacgg caacctctgc gagggcatcg gctcgaacat cttcacggtg
     3721 aaggaggggc gcatctcgac gcccgagggc cgctacgtgc tcgaagggat cagccgccag
     3781 accgtcatcg agctcgcgcg gaagctcggc tacgaggtgg tcgagcgcgg catcgacctc
     3841 tatgacgcat acactgcgga tgaagtgttc ctcacctcga ccagcctctg catctgcccg
     3901 gtggcgagcg tcaacggcaa ccggatcgcc ggcggcaagg taccgggggc agcgacgaag
     3961 cgcattctcg acgcctactc ggacatggtc ggctgcgaca ttcaggggca gtacctggct
     4021 cgtctgtgat cggatatggc gattggatcg cgtctcgcgt gagtcgatcg ccaatcgaac
     4081 cggacagacc cagcatgcgc agatatctcg cggcgctcgt cgtagccgtc gccaccttcg
     4141 ccctggtggg cagctcgccg gccgaggcga agaagctctg cttcggcacg gacgaacatc
     4201 tccgcttcgt gcagaagctc gatgccaagg gaccgaacgg cgagcctttg gaactcgccc
     4261 gcaagatccg catggaatgc ttcgtcgcgc cctacaccgt ggcggatgac ggctacgtgc
     4321 tgaagatcgt cggcgccaat cgatacttct cgctgacgcc ggagatcatc gccggcatgc
     4381 agaaggcgaa ccaactgccg aaccccatgc cgccctacca gttggatacg atggaccgca
     4441 tcttcggcca tctgctctgg atcttgatcg gcgtcttcgc gctcgtgagc ctcgtcagct
     4501 ggctgcgcaa gcgctcgcgc gcttgatcga ctaggtttcc aaaggccaag gcctttggcg
     4561 gggttcgggg cagtgccccg accttacctg atcacggcct ccacgcggtg gcggcagcct
     4621 tcacctcacg caaacgatag ctgcaccttc atcgagcgac cacggtccga ggccagaccg
     4681 aaggcctcca ccgccttctc gaccggcagt gtcgccgtga tcagcggacg cacatcgacg
     4741 aggcgcttgc ccagcagctc cgtcgcgagc ccgtactcct cgtggaagcg gaaggtgccg
     4801 cgcacctcga tctccttggc gacgatggcg ttcatgggga gcgccatctc gccgccgagg
     4861 ccgacctgca cgatgatgcc gcagggcttc aggacatcga tcgctgcggc gacggccggg
     4921 gctgcaccgg acgcctcgaa ggcgacgtcg aacgtgccct tgtccttcgc gtagtcggcg
     4981 agcgccgcct tgtccttggc gacgttgacg aatcggtcga cgccgagctt ctccagcgtg
     5041 acgcgagggg catctgagat gtccgtcgcg acgatcagcg aggcgccagc gcggcgcgcc
     5101 gcgatcgcac acagagcgcc aatgggcccc gaacccgtca cgatcacctt gcgcccgagc
     5161 agcggaccgg cccgccgcgc ggcatgcagg cagaccgcga gcggctcggc catggcagct
     5221 tcgcctgctg acaggctctc cggcacgaca tggcactggc cggcctcggc gacgatcgcg
     5281 tcgcggaatg cgccctggac gtggggaaag cgcatcgcgc ttccgtagaa gcgcatatcg
     5341 aggcagtgca tctgcaggcc cgcctggcaa tagcggcacg ccccgcaggg ccggctcggg
     5401 ttcagcgcga cacggtcgcc gaccttcacg ctcgtcacgc ccgcgccgag cgcctcgatt
     5461 cgccccgcca cctcgtgacc gagcaccatc ggctcgcgga ccacgatcgc ggtgccgatg
     5521 cgaccgtcat gatagtagnn nnnnnnnnca cctcggccag gatgtcgatc tcgcgctcga
     5581 agagccgcag atgccgcccg cctcgatacg cacgcgcacc tggccgggac cgggcgcacc
     5641 cgtctcgatg cggtcgatgc gcaggtcctt ggcggcatgg atgacgacgg cgcgcataag
     5701 ctcctcccgg ctcgtgtcgt cgagagatcg cacctgatcg ggtggcgagg caagggcagc
     5761 ggtgcgcgtt ggtgccacgg ggcgtcgtca tgagggttgg tcggctcgac cggcgctgat
     5821 agcttcccgg catgcaacga gccgaaattc tccgctttgc tccgagcccg accggtgcct
     5881 tgcatctcgg cagcgcgctt tcggcgctga cggggttcgc gatcgcgcga catctcggcg
     5941 gccgctttct cgtgcgcatg gaggataccg accagacccg ctgccggccg gagctcgccg
     6001 cggcgatcct cgccgatctc gcctggctcg gcgtgtcgtg ggaggagccc gtcctcgtcc
     6061 agtcgcagca ctacgatgtc tatcgccagg cagcggcgcg gctcgaggcg atggggctcc
     6121 tctatccctg ctttgcgagc cggaccgaaa tcctggcagc ggcggacccg aacgcacgcg
     6181 atcccgacgg cgcgcccctc tatccgcggc tacacaaggg aatggcgccc gtcgaggtcg
     6241 cgcggcggcg ggcgcgagat gagccggtgg cactgcggct cgacatggac aaggcgctcg
     6301 cggcggcgac ccggttgctt ggaggcgcgc tgctcacctt cgaagcgctc gatgaatacg
     6361 gtcaaccgac gactgtgacg gcacagcccg agcgctgggg tgacgtcgtc atcgtcggca
     6421 aggagcggcc gacgagctat cacctctccg tcgtcgtcga cgatgcccgc cagggcgtga
     6481 cgcttgtcac gcgagggctc gatctactgg ccgcgaccga cctgcaccga ctcctccagg
     6541 tgctgctcaa cctgccggcg ccgcgctacc atcaccaccg gctggttctg gccgaggatg
     6601 gcaagaagct ctcgaaaagc gcgcgggata cttcgatcgc cgacctccgc gcggccggcg
     6661 cctcgcctgc cgacatccga cgcctgatcg gcccgatcga cctcagctcc tggccgcggc
     6721 gatgaagcga cggatgaggt ccgcatcctt gacgccggga ctagtctcga cgcccgagga
     6781 gacgtcgacg aaggcgggac cgacgagccg cacggcgtcg cggacattct ctgctgtcag
     6841 gccgcccgcc agcatgaacg gacggcgcac gcccggcgca tcgaggagcc gccagtcgaa
     6901 cggcacgccg ttgccgccag ggagcggcgc actcggcggg ggcttcgcat cgaagaggag
     6961 gaggtcggca gcgcctgcgt cgagatagtg ggccgcgcgc tcggcatcgc cggggctcga
     7021 gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnngtgcaa tgttgcgcgg gctcttcggg aagaagacga ggccgaagag
     7261 gtcggcgccg gcctcgcgcg cggcggaaag cgcgtcgctg gtgcggatgc cgcagatctt
     7321 gatgccgatg ctcatggcga gggatgtagg cggccgtgtc cgcggcgtca aatgtcgctc
     7381 gcgagatcag cgccaagcat tcgtcgtccg ttctcgaagt attcgcggca atcagccagc
     7441 gacgacttgg gcgggcacag aaaattggca ggctatggcg cgaaagtgtg ttgctggcgg
     7501 ggaaaggggg gatatagtgg aaatgttgcg tgaggttgct caatgcgtat cggggggctc
     7561 aggccgaggt cgtcggcgcc agcggtggca ggcgcaatgg cggagcatgg ctcaacccgg
     7621 tccaagcggc gtcatgccgg caagctcgtt cgcctgatca tggcgggcac gttcgccatt
     7681 ctcggtgtct ccaccgtatc cgtaatggtc tccgcgggag ccgactacgg gttcgtgcgc
     7741 tccaccagca gctggatcga gaagacgtca ctgctctcct ggactggcga caacaatgcc
     7801 aagcaggcga tcacggcaac cggcgcatcg gcactgatcc tgcttcttgc agcgttctac
     7861 gtttgcggac tggggcgctg gcagtccgag ttccgctacg acaagcgcgc gcacgacctc
     7921 ctctactatc tcgggttcat cggcacgctt tcgggcctag gtgccgccgc gctgacctta
     7981 gtgatgaccg agaaagccgg ctcgcccaag gtgaccatgc tggttgcgca gaacggcgtt
     8041 gccctgatga ccacgcttgt cgggctcctc ggacgcaatc tgttgtccta catgtttcca
     8101 ccgcccgagg ccgtcgagcg gacggcggcg gagatcggcc gcatcaacgc cgagctgcag
     8161 accctcggca ccaccgtgaa aggcgtcagc ggcaatttct cggccttctc ggacaccgtg
     8221 acgggcacgc gcgcggccat cgaggctcac cacgagccct gggagcggct ccgccagcag
     8281 gccgagcaga cacatgcccc gctgaccgcg gcggcgcaga gtttcggcgc ggtcgcggga
     8341 tcagtcgggg cgctctcggg ccagctgcag ggcagcctcg acgcggtcgg ccggctcgcc
     8401 gcctcgacgg gggaggtcgc gaccc
//
LOCUS       DAORNH010000202         8412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6250_length_8412_cov_2.447888, whole genome shotgun sequence.
ACCESSION   DAORNH010000202 DAORNH010000000
VERSION     DAORNH010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_8412_cov_2.447888"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK264_17725"
     CDS             complement(<1..261)
                     /locus_tag="PK264_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77748.1"
                     /translation="MTEDDLKNRIAGCQELPARAEPRRRAIEAALQSFDRAQAVDMAP
                     ERELVVLHLRSPRAGAQSAMATAPDAARNVIMLPARAPSMKEP"
     gene            complement(273..791)
                     /locus_tag="PK264_17730"
     CDS             complement(273..791)
                     /locus_tag="PK264_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRD77749.1"
                     /translation="MTLAERASRGDRLAFSALLERHYPRIVGRAWRVTGRRDLAEDIA
                     QDVCVKLTSAIKGYRGEARFTTWLYPIIHTTAIDGLRRSGREDATETDEIVRLLDGTG
                     AELPADERLAGAELWAAVRRLSPKQRDAVLYVYGEEMSHAEAAELLNCSEATVSWHLH
                     EARKRLKTLIES"
     gene            1377..1874
                     /locus_tag="PK264_17735"
     CDS             1377..1874
                     /locus_tag="PK264_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRD77750.1"
                     /translation="MATAETTYARDIHPVDLVEQMALGHDWAFERTCDDELTLTVAGS
                     WTDYNVSMNWRDDLETLHIACAFDFKVPENRLVEVYRLIAQINEQLWLGHFDFWHHDG
                     LIMFRHGLMLNGAMATPSQCEALLRAALDACERHYQAFQFVVWAGKDAKQALASIMFE
                     TEGQA"
     gene            1991..3370
                     /gene="mgtE"
                     /locus_tag="PK264_17740"
     CDS             1991..3370
                     /gene="mgtE"
                     /locus_tag="PK264_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRD77751.1"
                     /translation="MQETTTAAAVGAVTGPVTAERVADVIAALAREDGDLVALTRDLN
                     APDVADLLALLEPDQRVQLIQRLGPAFDVEVFSELDETVRDQLSEAIPNETLAKVVGE
                     LETDDAAYLIEGLASEDRKEILDALETSDRVALERNLEYPEETAGRIMQSDFVAVPPF
                     WTVGQVIDHMRESEELPDTFSDIFVVDPTHRVLGTIDLSRLLRTKRQVGIDQIMSTER
                     HLVLATSDQEEVARQFERHDLKSAPVVDANRRLVGVVTVDDMVDVMRDEADEDIKALA
                     GVGDEDVTDNVVDTARSRITWLVVNLFTAMLASTVIRMFDATISQMIALAALMPIVAS
                     MGGNAGTQTMTVTVRALATNKLGPVNAIRMVTREALVGLLNGLVLALIVSVVVFFWFG
                     SGKLGLVIGAAMIVNLMAAALAGILVPMTLNRYGLDPAPASGVFVTTVTDVVGFFAFL
                     GLAALWMTK"
     gene            complement(3373..3831)
                     /locus_tag="PK264_17745"
     CDS             complement(3373..3831)
                     /locus_tag="PK264_17745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HRD77752.1"
                     /translation="MADATEALFYHLERQPLDKVLPSLLERTLERGWRAVVQAGNAER
                     IEALDTLLWTYSEGSFLPHGTSRDGMPERQPIFLTTGDENPNQASVRFLVDGATPVTF
                     AGYTRVVLIFDGHDQGAVADARRHWKAAKEAGCQATYWQQGDDGRWQKRA"
     assembly_gap    3926..3949
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3950..5616)
                     /locus_tag="PK264_17750"
     CDS             complement(<3950..5616)
                     /locus_tag="PK264_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77753.1"
                     /translation="MTSTNRLGLAASLAALRAFADPPATLAQQPPPATHYVGRAACHE
                     MMLFTLSVAANGAARLSYRSGDVDQHVEVIEGKLGTGAVGFAFPAGETIRLKGAFSAD
                     RATMTATWHNAGGEPEVDCGKAEFARLALSPEKHMEALITRLGEVAPSLDHAKEVAAL
                     SIGRPDLIGLPDLQAASLMAKHESARAGFWQRFWKLEQARILAMPAAAPADLKALDER
                     VATLAAPAMGLEYVAVRVPAIFTPGDFKDSGSFLLWATGLIADRKAALGQKPLPLTFG
                     GGGDPCTRIHWSGTIVEIKALEMIFAFPADYWDEGALASMRASLARCHPTAPEIVKDR
                     YTQAIGVLDGEAKEIEARTQGRAWLLAERLRMLALPLTVATLRATLDYELARDDVQAR
                     RIGATEVARFFEPGMASRRSEALAAAKREIEAAFVAAVPGQPSEDAAAALCESGPTMT
                     LGLNEHCRSTIASYVARVVGRVASEAKARIKAAPRTLAGLKANKGFRLDRSMLPASRF
                     DLLEGLERITHEFDKEIGPIRAEAIAAATKEILEAYIALDTNNPAAMR"
     gene            complement(5732..6895)
                     /locus_tag="PK264_17755"
     CDS             complement(5732..6895)
                     /locus_tag="PK264_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HRD77754.1"
                     /translation="MAYDLVLTGGRVIDPSQGLDGPADVAFKDGKVAAVAKGLKTEGA
                     KDVRSVKGYIVSPGLIDLHTHVYWGGTSLGIDAEEFCRNSGVTTSIDTGSAGPGNFLG
                     FRKHVIEPSKVRILAYLHVSFAGIYAFSNTIMYGESQSMQLMAPRDAAEVADKNRDLI
                     VGIKVRVGLHASGDQGTAPLNVALQVADEVGMPLMCHIDHPPPSYEAVLDMLRPGDVL
                     THAFRPFPNAPCTAQGSVKPAVIAARKKGVLFDIGHGKGSFSFKTTRAMLANGFMPDT
                     ISSDIHTLCINGPAFDQVTTMSKFLCLGVPLAEVIKQSTVNAGMALRRPELGTLKPGS
                     AGDATIISIAEGKFDYEDVLGEHMTGDRKIVAEGTVVAGKWFHTRKGAKITKG"
     assembly_gap    6933..6963
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6986..7474)
                     /locus_tag="PK264_17760"
     CDS             complement(6986..7474)
                     /locus_tag="PK264_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD77755.1"
                     /translation="MAIDALVRPFLALELFKGLTPLQITEIARRAERVVYKPGDVIME
                     KGAAGDAAIIVVGGTSVRTDGAMTADQCLPVPEGSLLGELAMLIEQTYGSTVVALTPV
                     KALRITRTAMHEMMLDDTRLADHFVQRISSRLKSVAAELRRIDSVLAMPFAASIEQPT
                     PA"
     gene            complement(7614..8306)
                     /locus_tag="PK264_17765"
     CDS             complement(7614..8306)
                     /locus_tag="PK264_17765"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRD77756.1"
                     /translation="MQLTDQARPLLRSEMRFPMRRAPVSEKPAKGRTKERVKEKRAGT
                     RVHPQSESLFQALKLLRIKLATEGNVPPYVIFHDRTIAEIAEKRPATPEALGEIIGLG
                     ERKVAKYGAALLEIVTNYPAAPPVDTRLSATVNATLGLYRQGMKPAAIARERTIEVST
                     VKGHLAEAIRLGLIEAADVLDLDSSELDQIEAAFERHGTRDTGDLGPVHAALRGQYDH
                     GTLKCVLAGGVR"
BASE COUNT         1339 a         2739 c         2794 g         1485 t
ORIGIN      
        1 gggctctttc atcgacggcg cacgtgccgg cagcatgatg acgttgcgcg ctgcatccgg
       61 cgccgtcgcc atggccgact gggccccggc gcggggcgag cgcaggtgaa gcacgacgag
      121 ctcgcgctcg ggtgccatgt cgacggcctg tgcccggtcg aaggattgca acgccgcctc
      181 gatcgcgcgc cggcgcggct ctgcgcgcgc cggcagctcc tggcagcccg caattcgatt
      241 cttgaggtcg tcttcggtca tcgtcgtcgg cctcagctct cgatcagcgt cttgaggcgc
      301 ttgcgggcct cgtggagatg ccaggacacg gttgcttccg agcaattgag cagctcggcc
      361 gcctcggcgt ggctcatctc ctcgccgtag acgtagagca ccgcgtcgcg ctgtttgggc
      421 gagaggcggc ggacggctgc ccagagctcg gcgccggcga gccgctcgtc tgccggcagc
      481 tctgcgccag ttccgtcaag cagacgcacg atctcatcgg tttccgtggc atcctcgcgc
      541 cccgacctgc gcaggccgtc gatcgcggtc gtgtggatga tcggatagag ccatgtcgtg
      601 aagcgcgctt cgccacggta gcccttgatc gccgatgtca gctttacgca gacgtcctgg
      661 gcaatgtcct cggcaaggtc gcgccggccc gtgacgcgcc aggcgcgtcc gacgatgcgc
      721 gggtagtggc gctcgagaag cgccgaaaaa gccagccggt cgccccggct cgcccgttct
      781 gccaaagtca tgtcatcatc cggcgtcgga ggcgtaagcc gcacctgacg gagctccatg
      841 gacgaggcct cgctcgcttt ggagtttagt ctacctgtcg gacgaccggg agactgaatt
      901 ccttgggggc cggcgccggt gcttgaccgc ggccacgcca atcgcggtcc gcgacgcgtc
      961 ggccagcaag cgcggcactg atgcatcacc ctgttgcaga ggcgacaccg ctcccgaaat
     1021 actggaaaga gccgcagtgg acggccggtt atcctcatgt cgtccacagc aaatcacttc
     1081 gtgtcctcgg caatttcgcg gcaaagcgcc tgtttttggg gtctctggga tagaaagcac
     1141 gtggagaagc cggggtgtca cttgccgcac ggagtccggt gcgtttaggt ccatgggcgg
     1201 caaatcactg ccaccaaatc aggcgcgccg ccgtggggac ggcgaacgcg ccggcggcgg
     1261 cagtctgttt tgatgatcgg ctggcccgac acgtcgagtt tgccgggact acagtttgtg
     1321 gcgaagtttg gtgtccggcg tcaggtctac tgcggcaatc ggaatgggga gcggggatgg
     1381 ctacggctga aacgacctac gcgcgggata ttcatccagt agatctcgtc gagcagatgg
     1441 cgctcgggca cgactgggcc ttcgagcgta cgtgcgacga cgaactcacg ctgacggtcg
     1501 caggcagctg gacagactac aacgtctcca tgaactggcg cgacgacctc gagacactgc
     1561 acatcgcctg tgccttcgac ttcaaggtgc ccgaaaaccg gctggtcgag gtctatcgcc
     1621 tcatcgccca gatcaacgag cagctctggc tcggccactt cgatttctgg caccacgacg
     1681 ggctcatcat gtttcgccat ggcctgatgc tcaacggcgc catggcgacg cccagccagt
     1741 gcgaggcgct gctgcgagcc gctcttgatg cctgcgagcg gcactaccag gctttccagt
     1801 ttgtcgtctg ggctggcaag gacgccaagc aggcactcgc ctccatcatg ttcgagaccg
     1861 agggtcaggc ctgacggtgc tcggcgtggt tgctctctga cactcgcgct atgtcgcctt
     1921 tgatcggcga cgaaactggt ccatacgggc ttccagccca gtggaatgcg accaaaccgc
     1981 gagcgacgga atgcaggaga ccacgacagc ggcggcagtg ggggcggtca cggggcccgt
     2041 gaccgccgag cgcgtcgcag atgtcattgc cgcgctcgcc cgcgaagatg gcgacctcgt
     2101 tgccctgacg cgggatctga atgcgcccga cgtggccgat ctgctcgctc ttctcgagcc
     2161 cgatcagcgc gtgcagctga tccagcggct cgggccggcg ttcgatgtcg aggtgttttc
     2221 cgagctcgac gagacagtcc gcgaccagct ctcggaggcg atcccgaacg agacgctcgc
     2281 caaggtcgtc ggcgagctcg agacggacga tgcggcctac ctgatcgaag gcctcgccag
     2341 cgaggatcgc aaggaaatcc tcgacgccct cgagacgagc gaccgcgtcg ccctcgagcg
     2401 caacctcgaa tacccggaag agacggccgg ccgcatcatg cagtcggact tcgtcgctgt
     2461 gccgccgttc tggacggtcg ggcaggtgat cgaccacatg cgtgaatctg aggagttgcc
     2521 ggataccttc tccgacatct ttgtcgtcga cccgacgcat cgcgtgcttg gcacgatcga
     2581 tttgagccgg cttctgcgga caaagcgcca ggtgggcatc gatcagatca tgagcaccga
     2641 gcgccatctg gtgctcgcga cctccgacca ggaggaggtc gcgcgccagt tcgagcgcca
     2701 cgatctcaag tcggcgcccg tcgtcgatgc caacaggcgc ctggtcggcg tcgtgaccgt
     2761 cgacgacatg gtcgacgtca tgcgggacga ggccgacgag gacatcaagg cgctcgccgg
     2821 cgtcggcgac gaggatgtca cggacaacgt ggtcgacacg gcacggagcc gcatcacgtg
     2881 gctcgtggtc aacctgttca cggcgatgct cgcctcgacc gtgatccgca tgttcgacgc
     2941 cacgatctcg cagatgatcg cgctcgctgc gctgatgccg atcgtcgcct cgatgggcgg
     3001 caacgccggc actcagacca tgacagtgac ggtgcgcgct ctggcgacca acaagctcgg
     3061 cccggtcaac gccatccgca tggtgacgcg tgaagctctg gtcgggctgc tcaatggcct
     3121 cgtgctggcc ctgatcgtct ccgtcgtcgt gttcttctgg ttcggctctg gcaagctcgg
     3181 cctcgtcatc ggggcggcca tgatcgtcaa tctgatggcg gcggcccttg caggcatcct
     3241 cgtgccgatg acgctcaatc gctacggtct cgatccggcg ccggcctcgg gcgtcttcgt
     3301 gacgacggtg acggatgtgg tcggcttctt cgccttcctc ggcctcgcgg cactttggat
     3361 gacgaagtag cctcaggcgc gcttctgcca gcgtccgtcg tcgccctgct gccagtaggt
     3421 ggcctggcag ccggcctcct ttgcggcctt ccaatggcgg cgagcgtcgg caaccgcgcc
     3481 ctggtcgtgc ccgtcgaaga tcaggacgac gcgcgtgtag ccggcaaagg tcactggcgt
     3541 cgcgccatcc accagaaagc gcaccgacgc ctggttcggg ttctcgtcgc cggtcgtgag
     3601 aaagatcggt tggcgctccg gcatgccgtc gcgtgatgtg ccatgtggca ggaagctccc
     3661 ctcgctgtag gtccacagca gcgtgtcgag tgcctcgatt cgctccgcgt ttcccgcctg
     3721 gacgacggcc cgccagccgc gctcgagtgt tcgctcgagc aggctcggca gcaccttgtc
     3781 gagcggctgg cgctccaggt gataaaagag cgcttcggtg gcatcggcca tgcaggcggt
     3841 ccgcggtggc tacttcttgc gcccaagata ggcggccatg cccttgcggc aagcgtcctc
     3901 gaggtcgcgg agattgaggc ctgcgnnnnn nnnnnnnnnn nnnnnnnnnc gcatcgccgc
     3961 cgggttgttc gtgtcgaggg cgatataggc ttcaaggatt tccttcgtcg cagcggcgat
     4021 cgcctccgcg cggatcggcc cgatctcctt gtcgaactca tgcgtgatcc gctcgagccc
     4081 ttccaggaga tcgaaccgtg acgcagggag catgctccgg tccaggcgga atcccttgtt
     4141 ggctttgagg ccagccagag tgcgaggggc ggccttgatg cgcgccttcg cctcgctcgc
     4201 cacgcggccg acgactcggg caacgtagct cgcaattgtg ctgcggcagt gctcgttcag
     4261 ccccagagtc attgtcggtc cgctctcgca gagggccgcc gccgcatcct cgctcggctg
     4321 cccgggcacg gccgcgacaa aggcagcctc gatctcgcgc tttgcggccg ccagcgcctc
     4381 gctacgccgc gatgccatgc cgggttcgaa gaagcgcgcg acctcagtag cgccgatacg
     4441 gcgcgcctgt acgtcgtcgc gggcaagctc gtagtcgagc gtcgctcgaa gcgtcgcgac
     4501 ggtcagcggc agcgccagca tgcgcaggcg ttcggcgagc agccaggctc ggccctgcgt
     4561 acgcgcctcg atttccttcg cttcgccatc gagaacgccg atggcctgcg tgtagcgatc
     4621 cttgacgatt tcgggtgccg tcgggtgaca gcgcgcaagg ctcgcgcgca tcgaggcgag
     4681 ggcgccctcg tcccagtagt ccgccgggaa ggcgaagatc atctcgagag ccttgatctc
     4741 gacgatggtg ccggaccagt gaatgcgcgt gcacgggtct ccgccgccac cgaaagtaag
     4801 cggcaacggc ttctgcccga gagccgcctt ccggtctgcg atgagtcccg tcgcccacaa
     4861 caggaacgag ccgctgtcct tgaagtcgcc cggcgtgaag atggccggca cgcgaaccgc
     4921 cacgtactcg agccccatgg caggggcagc gagtgtagca acgcgctcat cgagcgcttt
     4981 caggtctgcg ggagccgctg cgggcatggc gagaatgcgc gcctgctcga gcttccaaaa
     5041 tcgctgccag aacccggccc gcgcgctttc gtgcttggcc atcagcgagg cggcctgcag
     5101 gtccggcagg ccgatgaggt cgggccgacc gattgacagc gctgcgacct ccttggcgtg
     5161 atcaaggctc ggcgccacct cgccgagacg ggtgatgagc gcttccatgt gcttctcggg
     5221 cgagagcgcc agccgggcga actccgcctt gccgcagtca acttcgggct cgcccccggc
     5281 attgtgccag gtggcggtca tggtcgcgcg atcggcggag aacgcgccct tgaggcggat
     5341 ggtctcgccc gcgggaaatg cgaatccgac cgcaccggtg ccaagcttgc cttcgatcac
     5401 ctccacatgc tggtcgacat cgccgctgcg atagctgagc cgcgcggcgc cgttggcggc
     5461 aacggacagc gtgaacagca tcatttcgtg gcaggccgct cggccgacat agtgggtcgc
     5521 gggtggcggc tgctgtgcga gcgtggcggg gggatcggcg aacgcccgga gggccgcgag
     5581 cgacgcggca agtccgagac ggttcgtcga ggtcatgcag ggctctctcg gcttcgatgg
     5641 cacacggatc atccgccgac gctatcacac cggcgggcaa tcatgcttgt gccggcgaga
     5701 catggtttcg acagcgggtc gccgccccgc ttcagccctt ggtgatcttg gcacccttgc
     5761 gcgtgtgaaa ccatttgcca gcgacgacgg tgccctcggc cacgatcttc ctgtcgccgg
     5821 tcatgtgctc gccgagcacg tcctcgtagt cgaacttgcc ttcagcgatc gagatgatgg
     5881 tcgcgtcacc cgcagatccg ggcttcagcg tgccgagctc cggccgccgg agcgccatgc
     5941 cggcattcac ggtcgactgc ttgatgacct cggcgagcgg cacgccgagg cagagaaact
     6001 tcgacatggt cgtcacctgg tcgaaggcgg gaccattgat gcagagcgtg tgaatgtcag
     6061 aggatattgt atccggcatg aagccgttcg ccagcatggc gcgggtcgtc ttgaacgaaa
     6121 acgagccctt gccgtggccg atgtcgaaca gcacgccctt cttgcgggcg gcgatcacgg
     6181 cgggcttcac gctgccctgc gcagtgcacg gcgcattcgg gaacgggcgg aaggcgtggg
     6241 tcagcacatc gcccggccgg agcatgtcga gcacggcctc gtaggacggc ggcggatggt
     6301 cgatgtggca catcagcggc atgccgacct cgtccgccac ctgcagcgcc acatttagcg
     6361 gcgccgttcc ctggtcgccg gaggcgtgca ggccgacccg caccttgatg ccgacgatga
     6421 gatcgcgatt cttgtcggcg acctcggcgg catcgcgcgg cgccatcagc tgcatggact
     6481 ggctctcgcc gtacatgatc gtattcgaga acgcatagat gcccgcgaat gagacgtgca
     6541 ggtaagcgag gatgcgcacc ttgctcggct cgatcacatg ctttctgaag ccaaggaagt
     6601 tgccgggccc tgcgctcccc gtatcgatcg aggtcgtgac gccggagttg cggcagaact
     6661 cctcggcgtc gatgccgagg gaggtgccgc cccagtagac gtgcgtgtgc aggtcgatga
     6721 ggcccggcga gacgatgtag cccttgacgc tgcggacgtc cttggcgccc tcggtcttga
     6781 ggcccttggc gaccgcggcc actttgccat ccttgaaggc aacgtccgcc ggtccgtcga
     6841 gcccctgcga gggatcgatc acccgtccac ccgtcaatac gagatcgtat gccacgcgct
     6901 gtctccccga gccctgtgat ccaccgggcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnncatggcc gccgcagcag acgtgctatg cgggcgtcgg ctgctcgatc gaggccgcaa
     7021 acggcatggc gagcacgctg tcgatacggc ggagctcagc cgcaaccgac ttcaggcgtg
     7081 atgatattcg ctggacgaag tgatcggcga ggcgcgtgtc gtcgagcatc atttcatgca
     7141 tggcggtccg ggtgatccgg agcgccttga cgggcgtcag cgccaccacg gtggagccat
     7201 aggtctgctc gatcagcatg gccagctcgc cgaggagtga gccctcaggc acaggaaggc
     7261 attgatcagc ggtcatcgcg ccgtcggtgc ggacggacgt gccgccgacg acgattatgg
     7321 ccgcatctcc cgccgcccct ttttccatga tcacatcgcc cggcttgtat acgacgcgct
     7381 ccgcccgccg cgcgatctcg gtgatctgca gcggcgtcag ccccttgaag agctcaagcg
     7441 ccaggaacgg gcgcaccagt gcgtcgattg ccatgcttaa ccccacgcag cccccatgat
     7501 gccggagcac gagcacgcga cgcagaacag ttacgtatga ggggacggtc gtgcgattcg
     7561 caggcccaag tccagtcgcg ggcagcgcat cgcgagggtt gttcacagga cgctcagcga
     7621 accccgcctg ccaacacgca cttgagcgtg ccgtgatcgt actgcccgcg cagcgccgcg
     7681 tggacggggc caaggtcgcc ggtgtcgcgg gtgccgtggc gctcgaaggc ggcttcgatc
     7741 tggtcaagct cgcttgaatc gaggtcgagc acgtcggccg cctcgatcag cccgagccgg
     7801 attgcttcgg cgaggtgtcc tttgacggtc gacacctcga tcgtgcgctc gcgcgcgatt
     7861 gctgccggct tcatgccctg tcggtaaagc ccgagcgtcg cgttcaccgt agcgctgagg
     7921 cgggtatcga cgggtggggc cgccggataa ttggtcacga tctcgaggag ggccgccccg
     7981 tacttggcga ccttcctttc gccaaggccg atgatctcgc cgagcgcctc cggcgtcgca
     8041 gggcgcttct cggcgatctc ggcgatcgtg cggtcatgga agatgacgta gggcggcacg
     8101 ttgccttcgg tcgcgagctt gatgcgcaga agcttgagcg cctggaagag gctctcgctc
     8161 tgcggatgaa cgcgcgttcc ggcacgcttc tccttcacgc gctccttcgt gcggcccttc
     8221 gccggcttct cggagacggg cgcgcgccgc atcgggaagc gcatctcgct gcgcaggagc
     8281 ggccgcgcct ggtcggtgag ctgcaacgtg ccgtagccct ccgcgtcagc tgcgagatag
     8341 ccgcccgcac caggtcatca tgacgctgca acgcgcgcga catggccgcc ggcgcggatt
     8401 cccagaggcg gg
//
LOCUS       DAORNH010000203         8166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6494_length_8166_cov_2.524350, whole genome shotgun sequence.
ACCESSION   DAORNH010000203 DAORNH010000000
VERSION     DAORNH010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6494_length_8166_cov_2.524350"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PK264_17770"
     CDS             complement(<1..261)
                     /locus_tag="PK264_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HRD77757.1"
                     /translation="MTRAVLPREALVRFVAAPDGQIVPDLAERLPGRGVWVGCSRSVL
                     AEAVRSKAFARGLKRQVEHIIHILQTAEVAIVDDIGTEAPDGR"
     gene            complement(439..>1547)
                     /locus_tag="PK264_17775"
     CDS             complement(439..>1547)
                     /locus_tag="PK264_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HRD77758.1"
                     /translation="QIFLSRAKPEFMAKLFAMEVPEIYEGIVEIRAVARDPGSRAKIA
                     VETKDRSIDPVGACVGMRGQRVQAVVGELQGEKIDIIQWNDDPATFIVNALAPAEVAK
                     VVLDEESNRIEVVVAENQLSLAIGRRGQNVRLASQLTGWDIDILTEQEESERRQTEFA
                     ERTKLFMESLDVDEVIAQLLVTEGFATVEEVAFVDPAEIGNIEGFDADTATEIQTRAR
                     EYLDKLEAERDAKRRELGVHDDMAKVEGMTTAMLVRFGENEIKTVEDLAGAIPDDLVG
                     WVERKKPEKGEKAERGKDADVIRHKGALDGMPVSRAEAEQMIMSARVLAGWITEEDLA
                     RMAAEKEAAAAAEAATAEVAAGDAAASAAPASQP"
     assembly_gap    1548..1568
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1569..>1925)
                     /locus_tag="PK264_17780"
     CDS             complement(<1569..>1925)
                     /locus_tag="PK264_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HRD77759.1"
                     /translation="VENDATQINLPDAKKRKSDAAIGDELVEPLPPLDFGRVAAQNAK
                     QVIVQKVREAERERQFGEYKDRVGEIINGTVKRAEYGNVIVDLGRAEGIIRRDEMIPR
                     ENVRVNDRIRAFCYDVR"
     assembly_gap    1926..2036
                     /estimated_length=111
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2259..2969)
                     /gene="rimP"
                     /locus_tag="PK264_17785"
     CDS             complement(2259..2969)
                     /gene="rimP"
                     /locus_tag="PK264_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRD77760.1"
                     /translation="MTSASGSPSKERFIREQGLAAEVARLIEPSLEGLGFRLVRVQTS
                     GRDGLTLQIMAERHDGTMTVKDCETVSRNLSPVLDAADVIEGSYHLEISSPGIDRPLV
                     RASDFDDWAGYEAKIELKEPVSGRKRFRGTIEGLAEEEARIACEIEGEGLVVLGLPLA
                     LVDEAKLVLTDDLVREALRRAKRVREGRPEADELGDGMIADPDALEVEVGEAGPRTAK
                     PSPEGRGKKRRPSRPPPS"
     gene            complement(3375..4337)
                     /locus_tag="PK264_17790"
     CDS             complement(3375..4337)
                     /locus_tag="PK264_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HRD77761.1"
                     /translation="MATVQSALRTLEIEGEGLAALRAGLENGMRADFVAAVEMLRACA
                     GRVIVTGIGKSGHVGQKIAATFASTGTPAYFVHPTEASHGDLGMITKSDLILAISWSG
                     ETVELKNILTFSRRFSVPLIAVTSKRGSALGTHADVVLELPRAKEACPHGLAPTTSTT
                     MQLAIGDALAIALLEAKGFTARDFKVFHPGGSLGAQLKLVADIMHKGDALPLVAPDTR
                     VGEAIVVMSEKSFGCVGIVDAEGRLLGMITDGDLRRHMSTGLPDKHAAEVMTRNPNTL
                     EPDMPAPAALDEINRRKRTQMFVLDDGRPVGLVHVHDLLRLGVA"
     gene            4604..5527
                     /locus_tag="PK264_17795"
     CDS             4604..5527
                     /locus_tag="PK264_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD77762.1"
                     /translation="MLKCGAAAMAVIAAPRQPVFALAPAVKVGEFELATLSDGYLTLA
                     AQNFGVGIPADHVSLALGEAAKVGGKLDGTSVVAPVNVILVRRGADVTLIDAGAGGGF
                     MDTSGRLSQALEAAKIAPAAVTRVIFTHGHPDHLWGVIDEFDDSLRFPNAEYVMSEAE
                     WALWMTGDPASKVPATRSNFIPGAKRVLGRIKDRLKLVKPGQEAASGMVAVDSAGHSQ
                     GHMSIMLNSGGDRLLVLGDALIHPIVSFQYPEWPMAADHEPERGSAARRAILEVIINE
                     KLAFFGTHLPGGGFGRAERRAKAYNFLAGLK"
     gene            complement(5543..6490)
                     /locus_tag="PK264_17800"
     CDS             complement(5543..6490)
                     /locus_tag="PK264_17800"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HRD77763.1"
                     /translation="MKPMISLDDITFVGSGLARPECVLTTAKGEIFVGDHHAGIAEIG
                     KPKRKLLGAPPGFLPNGVTMLPNREFLIANLGPGGGVWKLDHDWRLSPWLLEVEGELL
                     RVCNFVGHERDGVVWISVSTRQYPREKAMRPGFGDGFICRVDGKGARIAADGIGFTNE
                     CRVDPTGRWLYVNETKAKCISRFPIEGGRLGTKEVLHRFGPGEFPDGFAFDSEGAAWV
                     ACVVANRVIRVETSGRATLVLDDGDSGLIAESEAIFNKGEGGRTHLEIGARRPLRNIA
                     SVCFGGPDLKTVYLGCLANDRIVSFRSPIAGAEPVCWRY"
     gene            complement(6536..7591)
                     /locus_tag="PK264_17805"
     CDS             complement(6536..7591)
                     /locus_tag="PK264_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77764.1"
                     /translation="MKKYNILMLGASYGSLLLLGGHSAHLICLPAEADLINKHGARVR
                     IPVKGRDGLVEINTQKLTGRLSAGGPAGVDPSTYDLVCLGMQEPQYRSPGVRELMERI
                     AKSKVPCMSIMNMPPLPYLARIPGLSLASLEPCFADASVWKPFDPTTMTLCSPDPQAF
                     RPPEEPVNVLQVSLPTNFKAARFQSDAHTSILRQLQADIEGARVDVGDGPFELPVKLK
                     VHDSIFVPLAKWAMLLTGNYRCVGKDGMRSIKEAVHTDVNASREVYTWVMALCRSLGA
                     AAEDLVPFEKYAAAANGLVRPSSAARALAAGAQNIERVDMLVRNIAAQKGLKNPVVEE
                     TVALVDGWLAKNRAKSA"
     gene            complement(7698..>7904)
                     /locus_tag="PK264_17810"
     CDS             complement(7698..>7904)
                     /locus_tag="PK264_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77765.1"
                     /translation="GLVTLPGMMTGQILAGIDPVDAAAYQLLIMFLISGATALAALLT
                     GLGAVRLLTDDRHRLRVDRIVPRG"
     assembly_gap    7905..7924
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7925..>8166)
                     /locus_tag="PK264_17815"
     CDS             complement(<7925..>8166)
                     /locus_tag="PK264_17815"
                     /inference="COORDINATES: protein motif:HMM:NF015605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77766.1"
                     /translation="GYAVGVVIGATPWWSPRYLLPILGMILGNALTSVSLVLETLTDA
                     ARRERPQIEARLALGATRFEALDGALKRALRTALMP"
BASE COUNT         1241 a         2675 c         2696 g         1402 t
ORIGIN      
        1 tcggccatcg ggcgcttcgg tccctatatc gtccacgatg gcgacttccg cagtttgaag
       61 aatgtggatg atgtgttcca cctgccgctt gaggccgcgt gcgaacgcct tggagcgcac
      121 ggcctccgca agcacggacc gtgagcaacc gacccataca ccccggcctg gaagtcgctc
      181 ggcgagatca gggacgatct ggccatccgg cgccgccacg aaccgcacca gcgcctcgcg
      241 cggcagcacg gcccgcgtca cgatgcacct ccgcgacggc tcggatgcag caggcgcggc
      301 ctcgccctcg ccgggttgtg cgttgccacc cggtgcgtcc ttgccgctgt gctgagccga
      361 ttgctgcatc aagtcctttc acatcgtgaa ttcgtgcgcc ggagtcggcg cgcgtcgggt
      421 ggccggaacc cgccgcgatc acggctggct tgctggcgct gccgacgcgg ctgcatcccc
      481 cgcagcgacc tcggccgttg ccgcttcggc cgcagctgct gcttccttct cggctgccat
      541 acgtgcgaga tcctcctccg tgatccagcc ggcaagcacg cgcgccgaca tgatcatctg
      601 ctcggcctcg gcgcggctta cgggcatgcc gtcgagggcg cccttgtggc ggatgacatc
      661 tgcatccttg ccgcgctccg ccttttcgcc cttctccggc tttttgcgct cgacccagcc
      721 gaccagatcg tccgggatgg cgccggcgag gtcctccacc gtcttgatct cgttctcgcc
      781 gaagcggacg agcatcgccg tcgtcatgcc ctcgaccttc gccatgtcgt cgtgcacgcc
      841 aagttcgcgg cgcttggcat cgcgctccgc ctcgagcttg tcgaggtact cgcgcgcgcg
      901 ggtctggatc tcggtcgccg tgtcggcgtc gaagccctcg atgttgccga tctcggccgg
      961 gtcgacaaag gcgacctcct cgaccgtcgc gaagccttcc gtcacgagga gctgcgcgat
     1021 cacctcgtcg acatcgagcg actccatgaa gagcttggtg cgctcggcga actcggtctg
     1081 gcggcgctcg ctctcctcct gctcggtcag gatgtcgatg tcccagccag tgagctgcga
     1141 ggcgaggcgc acgttctgcc cgcgccgtcc gatggcaagc gagagctggt tctcggcgac
     1201 caccacctcg atgcggttcg attcctcgtc gagcaccacc ttggcgacct cggccggcgc
     1261 cagcgcgttg acgatgaagg tggctggatc gtcattccac tgaatgatgt cgatcttctc
     1321 gccctgcaat tcgccgacga ccgcctgcac acgctggccg cgcatgccga cgcaggcgcc
     1381 gacggggtcg atcgagcggt ccttcgtctc gacggcgatc ttggcgcggc tgcccggatc
     1441 gcgggccacg gcgcggatct ccacgatgcc ctcgtagatc tcgggcacct ccatggcgaa
     1501 aagcttcgcc atgaactcgg gcttggcgcg cgagagaaag atctgcgnnn nnnnnnnnnn
     1561 nnnnnnnncc ggacgtcgta gcagaaagcg cggatgcggt cgttcacgcg cacgttctca
     1621 cgcgggatca tctcgtcgcg gcggatgatg ccttccgccc gcccaagatc gacgatcacg
     1681 ttgccgtact cggcgcgctt cacggtgccg ttgatgatct cgccgacgcg gtccttgtat
     1741 tcgccgaact ggcgctcgcg ctcggcttcg cgcacctttt ggacgatcac ctgcttggcg
     1801 ttctgcgcgg cgacgcggcc gaagtcgagc ggcggcaagg gctcgacgag ctcgtcgccg
     1861 atcgccgcgt cggacttgcg cttcttggcg tcggggaggt tgatctgcgt ggcatcgttt
     1921 tcgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggat
     2041 cgcgtcctcc atggcggtga tcacgatccg gcggtcaatg gacttctcgc gtgcgaccgc
     2101 gtcggcgatc tggagaagct cgagccgatt ggcgctgatg cctgcctggg ccatggtccc
     2161 aatcctcacg tctgatagag aagcgatagt cgtgtctggt gctgaacggt tgtctccgcc
     2221 gggtcgcact cggcgcgccc gcggccgaga cggcggcgct agctcggcgg tggtcgcgac
     2281 ggccggcgct tcttgccgcg accctcaggt gagggcttcg ctgttcgcgg tcccgcctcg
     2341 ccgacctcga cctccagcgc gtcgggatca gcgatcatgc catcgccgag ctcgtccgcc
     2401 tcgggcctcc cctcgcgtac ccgcttcgcg cgcctcagcg cttcgcggac gagatcgtcg
     2461 gtgagcacga gcttggcttc gtcgaccagt gccagcggca ggccgagcac gacaagccct
     2521 tcgccctcga tctcgcaggc gattcgcgct tcctcctcag cgagcccttc gatagtgccg
     2581 cggaagcgct ttctcccgga gacaggctcc ttgagctcaa tcttggcctc gtagccggcc
     2641 cagtcgtcga aatcggaggc ccgcaccagc gggcggtcga tccctgggga cgaaatctcg
     2701 aggtggtagc tgccctcgat cacatcggca gcgtccaaga ccggagatag gttgcgcgag
     2761 accgtttcgc aatccttgac tgtcatcgtg ccatcgtggc gctcggccat gatctgcagc
     2821 gtcagaccat cccgccccga cgtctgcacg cgcacgagcc tgaagccgag gccctcgagc
     2881 gacggctcga tgagcctcgc cacttccgca gccagtccct gctcgcggat gaagcgctcc
     2941 ttggacgggc tgccactggc gctggtcatg gaactcaagt gattccgttc actcacggtc
     3001 tggttaacgg cgctcatttt gcctgggtgc tcgatcgcaa acaaaaagtg cggacccggg
     3061 cggggcccac tccctgaagt tgagatgatc atgagcgtcc aacgaataca cgagcgcccg
     3121 gggaggcgca agcgcgtgtt tgcgggccgg gtttggtgca ctggctttgg ggccaggcgt
     3181 tgccggccac ccactactgt cgtgccggcg aacgccggcg cccaggtcga cccggatcgg
     3241 cccgcagacg tccctcgaac cgtggcctgg gcctcgtctt cgcgaggatg acgagggtgg
     3301 ggcgagccgt gcgcgagagc gtcgcctgcg tggccctgac ggatctggac agggccaggc
     3361 ttcaaggttg cgtttcacgc aacgccgaga cgcaggagat cgtggacatg cacgaggcca
     3421 accggccggc cgtcatcgag cacgaacatc tgcgttcgct tgcggcggtt gatctcgtcg
     3481 agggccgcgg gcgcgggcat atctggctcg agcgtgttcg ggttgcgcgt catcacctcc
     3541 gccgcatgct tgtcggggag gcccgtcgac atgtggcggc ggagatcgcc gtcggtgatc
     3601 atgccgagga ggcggccttc cgcatcgacg atgccgacgc agccgaagct cttctcgctc
     3661 atgacgacga tcgcctcgcc gacgcgcgtg tcgggcgcga cgagcggcag cgcgtcgccc
     3721 ttgtgcatga tgtcggcgac gagcttcagc tgcgcgccga gcgaaccgcc cgggtggaat
     3781 accttgaagt cgcgcgcggt gaagcccttc gcctcgagca gcgcaatggc aagcgcgtcg
     3841 ccgatcgcga gctgcatggt ggtcgaggtg gtcggcgcga ggccatgcgg gcaagcctcc
     3901 ttcgcgcgcg gcagctcgag cacgacatcg gcgtgcgtgc cgagtgccga gccgcgcttc
     3961 gaggtgacgg cgatcagcgg tacggagaag cggcgcgaga aggtcaggat gttcttcagt
     4021 tcgaccgtct cgccggacca tgagatggcg aggatcaggt cgctcttggt gatcatgcct
     4081 aggtcgccgt gcgaggcctc ggtcggatgc acgaagtagg cgggcgttcc ggtcgaggca
     4141 aaggtcgccg cgatcttctg tccgacgtgg ccgctcttgc cgatgccagt gacgataacg
     4201 cggccggcac aggcgcgaag catttccacc gccgcgacga aatcggcccg catgccgttc
     4261 tcgaggcccg cccgaagcgc ggctagtcct tctccctcga tctcgagcgt gcgcaacgcc
     4321 gactgcaccg tggccatgcc ggggtcccga tttgggcgcg ctttcttgcg gcctgccgtc
     4381 tcgctcatcg ccatcgtgcc tcttccggcg ttcgccgcat gattcgcctt tcgactgtaa
     4441 atgcaagacg gatattcccc aatggcgccg caatggccga tggctgctca gcctgcagtt
     4501 gtgaacaggc tgattggcaa cgccgaggcg gctgcctata tcggagttgg gaaaatccga
     4561 tccagggcgt gtcatgatgc cgagagcgcc gagccgtcgg acgatgttga aatgtggcgc
     4621 ggccgccatg gccgtgatcg ccgctccgcg ccaaccagtg tttgcattgg ctcctgcggt
     4681 caaggtcggc gagttcgagc ttgcgacgct gagtgacggc tatctgacgc ttgcggcgca
     4741 gaatttcggc gtcggcatcc cggcggatca tgtgagcctc gcgctcggcg aggccgcgaa
     4801 ggtcggcggc aagctcgacg gcacgtcggt cgttgccccc gtgaacgtca tcctggtccg
     4861 gcgcggcgcg gacgtaacct tgatcgacgc gggtgccggg ggcggcttca tggatacttc
     4921 cggccgcctc tcccaggcgc tcgaggccgc aaagatcgcg cccgcggcag tgacgcgggt
     4981 gatcttcacc cacggccatc ccgatcacct ctggggcgtg atcgacgagt ttgacgacag
     5041 cctgcgtttc ccgaacgccg agtacgtgat gtcggaggcc gagtgggcgc tctggatgac
     5101 cggcgacccg gcatcgaaag tgccggccac gcgctcgaac ttcattcccg gagcaaagcg
     5161 cgtgctcggc cggatcaagg accggctgaa gctcgtgaag ccggggcagg aggcggcatc
     5221 cggcatggtc gcggtcgaca gcgctggcca ctcgcagggc cacatgtcga ttatgctcaa
     5281 ctctggcggc gatcgtcttc tcgtactcgg cgacgcgctg atccacccga tcgtctcgtt
     5341 ccagtatccg gaatggccga tggcggccga tcatgagccg gagcgcggct cggcggcgcg
     5401 gcgggcgatc ctcgaggtga tcataaacga gaagctcgcg ttcttcggga cgcacctgcc
     5461 gggtggcggg ttcggccgcg ccgagcggcg cgccaaggcc tacaattttc tcgccggtct
     5521 gaaatagcga ttgcgagccg cctcagtagc gccagcagac gggctccgca ccggcaatcg
     5581 gcgagcggaa cgagacgatg cggtcgttgg cgaggcagcc gaggtagacg gtcttcagat
     5641 cgggcccgcc gaagcagacg ctggcgatat tgcggagcgg tctcctcgcg ccgatctcga
     5701 gatgcgtccg cccgccttcg cccttgttga agatggcctc ggattcggcg atcaggcccg
     5761 aatcgccatc atcgagaacg agtgtcgcgc gcccgctcgt ctcgacgcgg atgacgcggt
     5821 tggcgacgac gcaggcgacc caggcagcgc cctcgctgtc gaaggcgaag ccgtccggaa
     5881 actcgcccgg tccgaagcgg tgcagcacct ccttggtgcc gagccgcccg ccctcgatcg
     5941 ggaagcgcga gatgcacttc gccttcgtct cgttgacgta gagccagcgg cctgtcggat
     6001 cgacgcggca ctcgttggtg aagccgatgc cgtcagcggc gatccgcgca cccttcccgt
     6061 cgacacggca gatgaaaccg tcgccgaagc ccggccgcat cgccttctcg cgcggatact
     6121 ggcgcgtcga aaccgagatc cacacgacgc cgtcgcgctc gtggccgacg aagttgcaca
     6181 cgcgtagcag ctcgccctcg acctcgagca gccagggcga gaggcgccaa tcgtgatcga
     6241 gcttccagac gccgcctccc ggaccgaggt tcgcgatcag gaattcgcgg ttcggcagca
     6301 tggtgacgcc gttcggcagg aagcctggcg gggcgccgag aagcttgcgc ttcggcttgc
     6361 cgatctcggc aatgcccgcg tggtgatcgc caacaaagat ctcacccttg gccgtcgtca
     6421 gcacgcactc gggccgggcg aggccggaac cgacgaacgt gatgtcatcg agcgaaatca
     6481 tcggcttcat gtgtccggct ccggccatcg cactctcccg tccacgtgtc cgcgatcagg
     6541 cgctcttcgc gcggttcttc gcgagccagc catcgacgag cgcgacggtc tcctcgacga
     6601 cggggttctt caggcccttc tgcgcggcaa tgttgcgcac cagcatgtcg acccgctcga
     6661 tgttctgcgc gccggcggcg agcgcacgcg cggccgaaga cgggcggacg aggccgttgg
     6721 cggcggccgc gtacttctcg aacggaacga ggtcctcggc ggcggcgccg agcgagcggc
     6781 agagcgccat cacccaggta tagacctcgc gcgaggcatt gacgtcggtg tgcacggcct
     6841 ccttgatgga gcgcatgccg tccttgccga cgcagcgata gttgccggtg agcagcatcg
     6901 cccacttggc gagcggcacg aaaatgctgt cgtgcacctt gagcttcacg ggaagctcga
     6961 acgggccgtc gccgacgtcg acccgcgcgc cctcgatgtc ggcctgcagc tggcggagga
     7021 tcgaggtgtg cgcgtcggac tggaagcgcg ccgccttgaa gttcgtcggc aggctcacct
     7081 gcagcacgtt gaccggctcc tcgggcgggc ggaaggcctg cggatcgggg ctgcagagcg
     7141 tcatggtggt gggatcaaag ggcttccaca cgctcgcgtc ggcgaagcag ggctcgaggc
     7201 tcgcgagcga gagccccggg attcgggcga gatacgggag aggcggcatg ttcatgatgg
     7261 acatgcacgg cactttggac ttggcgatcc gctccatcag ctcgcgcacg ccgggcgagc
     7321 ggtactgcgg ctcctgcatg ccgaggcaga cgagatcata ggtgctcggg tcgacaccgg
     7381 ccgggccgcc ggccgaaagc cttccggtga gcttctgcgt gttgatctcg acgaggccat
     7441 cgcggccctt caccgggatg cgcacgcgcg cgccatgctt gttgatgagg tcggcctcgg
     7501 cagggaggca gatcaggtgc gcggagtgtc cgccgagcag gagcagcgac ccgtaggacg
     7561 cgccgagcat caggatgttg tacttcttca tgcgccatct gctccagctg aggtccaagt
     7621 cgtggatgga cgatgctagt cccgaagggg ccggtgacgc aaacgccaat gctcgtttgc
     7681 atgcctcgat ccgccgctca ccctcgcggc acgatccggt ccacgcggag ccggtggcgg
     7741 tcgtccgtca ggaggcggac cgcaccgaga ccggtgagca aggctgcaag cgcggtcgcg
     7801 cccgagatca ggaacatgat gagcagctga taggccgcgg cgtccacggg gtcgatgccg
     7861 gcgagaatct ggccggtcat catgccgggc aaggtcacga ggccnnnnnn nnnnnnnnnn
     7921 nnnnatcggc atcaacgccg tcctgagcgc gcgcttcagt gcgccatcga gcgcctcgaa
     7981 gcgcgttgca ccgagcgcca gccgtgcctc gatctgcgga cgctcgcgcc gtgccgcgtc
     8041 cgtcagcgtc tcgagcacga ggctgacgct cgtcagcgca ttgccgagga tcataccgag
     8101 gatcggcagc aagtaccgcg gcgaccacca gggcgtcgcg ccgatcacga cgccgacggc
     8161 gtagcc
//
LOCUS       DAORNH010000204         8157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6504_length_8157_cov_2.657986, whole genome shotgun sequence.
ACCESSION   DAORNH010000204 DAORNH010000000
VERSION     DAORNH010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_8157_cov_2.657986"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK264_17820"
     CDS             <1..351
                     /locus_tag="PK264_17820"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77767.1"
                     /translation="PPKGRSAATAAVVPPAVAAPGAGPDAERAATFKSRGDKSMKVGG
                     IAEARSFYERAAELGSADAAFMLAQTYDPRELKRLGAVGTAADIALARTWYERAAALG
                     HRDGAARAARLNVR"
     gene            complement(352..1620)
                     /locus_tag="PK264_17825"
     CDS             complement(352..1620)
                     /locus_tag="PK264_17825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HRD77768.1"
                     /translation="MLLGCIADDLTGATDLALMLTRAGMRTLQIMDVPGPATPLEGYD
                     AVVVALKTRTCPVEEAVRLSLASADALLARGARQLFFKYCSTFDSTDRGNIGPVADAL
                     LERVGGDLAIACPAFPTNKRTVYLGNLFVGEVPLAESPMKDHPLTPMRDSSLVRVLQR
                     QTRGKVGLVPFSVVEKGPRAIADAFSAARSRGERYVIVDAITDKHLVDMGAAAAKMAL
                     ITGGSGVALGLPANFIAAGLMTSKSAPTTMKAPAGRALIVAGSCSEATRGQVKAAKAA
                     GIPAFEVDPLGLHEGRTMCADIVRWAKSQPADRPVLVYSSADPASVKSVQEKLGREAA
                     GALVERELATVARALVGDGFSRIVVAGGETSGAVVNGLGVTALEIGPEIDPGVPWTTS
                     RGSSVPLALALKSGNFGGPEFFTSAWAKLS"
     gene            complement(1712..2362)
                     /locus_tag="PK264_17830"
     CDS             complement(1712..2362)
                     /locus_tag="PK264_17830"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HRD77769.1"
                     /translation="MAMMRRQTTGCQQRNHATGRCWRLLLALMIVAIAPAGRANAAPY
                     VFDRDHTSITFSWNHLGLSRQAGRLLDHEGTLEFDPAEPEKSNVDVTLRPSSIWTGVA
                     ALDKHLKSADFFDIGRFPAITFKSTAVKKTGERTGEVTGDLTILGTTKPVTLQVTWNF
                     TGEHPLAKLNPAYREKLVSGFSATARLLRSEWGMKRGMPLISDEIIVTIETELFRK"
     gene            complement(2435..3610)
                     /locus_tag="PK264_17835"
     CDS             complement(2435..3610)
                     /locus_tag="PK264_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRD77770.1"
                     /translation="MFTSFFLELKAAKVPVTLREYLTLMEALEADLADRRVEDFYYLS
                     RATLVKDERHLDKFDQVFGHVFKGLELMSDAAEARIPEEWLRAVASLYLTEEEKKKIE
                     ALGGWEKIMEELKKRLAEQKGRHQGGKKWIGTQGTSPYGAEGYNPAGVRIGQKENRNF
                     RAMKVWDKREYKDLDDSVELGTRNIKVAMRRLRKFAREGAHEELDLDGTIRGTASKGY
                     LDIILRPERRNTVKVLMFFDIGGSMDWHVKLAEELFSAARAEFKHMEHFYFHNCLYEF
                     VWKENARRWTDRKPTWDVLHKYPADYKVVFVGDASMSPYEITVPGGSVEHMNEEPGQA
                     WMSRVTDIYEHAVWLNPVPEQHWDWTPSIKLMKQLMGNRMYPLTLRGLDEAMRELMR"
     gene            3758..4582
                     /gene="proC"
                     /locus_tag="PK264_17840"
     CDS             3758..4582
                     /gene="proC"
                     /locus_tag="PK264_17840"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRD77771.1"
                     /translation="MALSLDGTLLLAGAGNMGGALLAGWLEQGLAPSRIIVQDPSPPP
                     RMTELLAKHRIRTEASIDRIDVPPAVMLVAVKPQIMDEVFPKLAKLAGPATVVLSVAA
                     GKTIAGFERHLAPGAAVVRSIPNTPASVGRGITVAAANANVTAAQKAACQKLLESVGA
                     VAWVDDEALIDPVTAVSGSGPAYVFYLCETLAKAGVAAGLEPGLALQLARWTVAGAGE
                     LLHRSDLTPDVLRQNVTSPNGTTFAALQVLMGEKGIQPVMTEAVAAATKRSRELAK"
     gene            4829..5083
                     /locus_tag="PK264_17845"
     CDS             4829..5083
                     /locus_tag="PK264_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HRD77772.1"
                     /translation="MAEKGDRYWARTSTLMWIMLILWLFFSFGVHMFVDKLNAFSFLG
                     FPLGFYMAAQGSLIAFVVMLFVFAKRQETIDTEEGEAEDA"
     gene            5097..6965
                     /locus_tag="PK264_17850"
     CDS             5097..6965
                     /locus_tag="PK264_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HRD77773.1"
                     /translation="MAAQTASGKRGFLENLGKIYGLYTGGFFAFVIVLAVLEQMGLDR
                     RFIGYGFVFLTLAVYAVIGILSRTMEAGEYYVAGRTVPAFYNGMATGSDWMSAASFIG
                     MAGNLYLQGYDGLAFVLGWTGGYVLVAILIAPFLRKFGAYTVPDFFSERYGGGLARFL
                     AVIVLIVCSFTYVVAQIRGTGLIAQQFLDMDYNVAVYVGLGGILLCSMLGGMKAVTWT
                     QVAQYIVLIIAYLTPVIILSAKKFNIPVPQITYGSAIQQITELEQSMLKAGLADPKVL
                     KPHATPFLVRDAMNYFLLILCLMVGTASLPHILMRYFTTPSVRDARRSVAWSLFFIFL
                     LYFTAPAYAAFAKLEVYTLINKKVAIADLAKEANWLFTYGKYGLVKICGANATGVDAV
                     VAACQKIAGHGGFLRHQDLAIHADSIVLSTPEIAKLPYVITGLVAAGGLAAALSTADG
                     LLLAIANALSHDLYYKLIAPNSSTATRLILSRILLLVVAVIAAWCATWFPPDILILVS
                     WAFSLAAAGLFPALVLGVWWKGATTTGAVLGMAAGFLVTGAYMIGTHWYKWPLWYGVS
                     NLSAGTFGLIVGFATIIIVSLCTKKPSAEVQAMIDATRRPRGDAIIKDKDAAIAAH"
     gene            7069..7380
                     /locus_tag="PK264_17855"
     CDS             7069..7380
                     /locus_tag="PK264_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77774.1"
                     /translation="MSPSESLIQHWYFHLPNLALAALMYTLIAAYLIAMFFKPDSEAV
                     ILRVFNSIANPFLKLVRTATPAIVPDGLMIVFAIVWLMALRMFWFLTCLAAGMRLQIG
                     G"
     gene            7384..7728
                     /locus_tag="PK264_17860"
     CDS             7384..7728
                     /locus_tag="PK264_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77775.1"
                     /translation="MSDDMDSRELTMQRDILMVATAGASLLNGQAFSPVFDPVFFLLK
                     PFLAAAFYSLPMVAFYLTSIFISLVTLMIAGIPAALYERAKGLKESSPISLGIWLAAT
                     IALVSLPYLMRA"
     gene            7829..>8157
                     /locus_tag="PK264_17865"
     CDS             7829..>8157
                     /locus_tag="PK264_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HRD77776.1"
                     /translation="MTAMHGLTAMVKGEILSPFTRVRRLLDGIPAGHAKPIEMTAGDP
                     KETMPGFVVDRIAEAKQLLGTYPHIRGSDELRKTIASWIERRYGLAGKIDPVREVHPV
                     NGSREGL"
BASE COUNT         1363 a         2711 c         2518 g         1565 t
ORIGIN      
        1 ccgcccaaag gtcgctcggc cgcaacagct gcggtggttc cccctgctgt cgcggccccg
       61 ggtgccggac cagatgcgga acgcgcggcc actttcaagt cgcgcggcga caagtcgatg
      121 aaagtcggcg gaattgccga ggcgcggtcg ttctatgaga gggcggcaga gctcggcagt
      181 gcggacgcag ccttcatgct cgcccagacc tacgatccgc gcgagctgaa gcgcctcggt
      241 gccgtcggca cggcggccga cattgcgctg gcccgcacat ggtacgagcg cgccgcggca
      301 cttggtcatc gcgatggcgc ggccagggcc gcacggctca acgttcgctg atcacgaaag
      361 cttcgcccac gccgacgtga agaactccgg accgccgaag ttgccagact tcagcgcaag
      421 cgcgagaggg accgagctac cgcggctcgt cgtccacggc acgcccggat caatctcggg
      481 tccgatctcg agcgccgtga caccgagccc gttcaccact gcgcccgagg tctcgccgcc
      541 ggccacgacg atgcgcgaga acccatcgcc gacgagcgcg cgcgcgactg ttgcgagctc
      601 gcgctcgacg agcgctcccg cggcctcccg tccgagcttc tcctgcacgg acttgacaga
      661 ggccggatcc gcgctcgagt agacgagcac ggggcggtca gcgggctgtg acttcgccca
      721 gcgaacgatg tcagcacaca tcgtgcggcc ttcatgcagg ccgagcggat cgacctcgaa
      781 tgccggaata ccagccgcct tggcggcctt cacctggccg cgcgtcgctt ccgaacaact
      841 gccggcgaca atcagcgcac gcccggccgg agccttcatc gtcgtcggcg ccgatttgct
      901 cgtcatgaga cccgccgcga tgaagttggc cggcaatccg agtgccacgc ccgagccgcc
      961 cgtgatcagc gccatctttg ccgccgctgc gcccatgtcg acgagatgct tatccgtgat
     1021 ggcatcgacg atgacgtagc gctcgccccg cgaacgcgcc gccgaaaagg catccgcgat
     1081 cgccctcggc cccttctcga ccacggagaa cggcaccaaa ccgaccttgc cgcgtgtctg
     1141 tcgctggagg acgcggacga gactcgagtc gcgcatcggc gtcagggggt ggtccttcat
     1201 cgggctctcg gcaagcggca cctcgccgac gaacagattg ccgagataga ctgtccgctt
     1261 gttggtcggg aatgccgggc aggcgatggc aaggtcgcct ccgacccgct ccagcagcgc
     1321 gtccgccacc ggaccgatgt tgccgcgatc ggtcgaatcg aaggtcgagc agtacttgaa
     1381 gaacagctgc ctcgccccgc gcgcgagcag cgcgtccgcg ctggcgagcg agagccgcac
     1441 cgcctcctca accgggcagg tgcgcgtctt cagggcgacg acgacggcgt catagccctc
     1501 gagcggtgtc gctggacccg gcacgtccat gatctgcagc gtgcgcatgc cggcacgcgt
     1561 cagcatgagt gcgaggtccg tcgcgccggt gaggtcgtcg gcaatgcatc cgagcagcat
     1621 tttggcctct gtgagggata ggcgttcgcg ggtggactgc acgctacatg gtcgatggcc
     1681 gccaggccag ccgctgccct cgcacagagc gctatttgcg gaagagttcg gtctcgatcg
     1741 tgacgatgat ctcgtcggag atcagcggca tgcctcgctt catgccccac tcgctcctca
     1801 agagccgcgc cgtcgccgag aatcccgaga ccagcttctc gcgataggcg ggattgagct
     1861 tcgccagcgg gtgctcaccc gtgaaattcc aggtcacttg cagcgtgacg ggcttggtag
     1921 tgccgaggat cgtcaagtcg cctgtcacct ctcccgtgcg ctcgccggtc ttcttcacag
     1981 ccgtgctctt gaaggtgatg gcgggaaacc ggccaatatc gaaaaaatcc gcagatttca
     2041 gatgcttgtc gagcgccgcg actccggtcc agatgctgga cggcctgagc gtgacgtcga
     2101 cgttcgactt ctcgggctca gccggatcga actcgagtgt accctcatga tcgaggaggc
     2161 gcccagcctg ccgtgagagg cccaagtgat tccacgagaa cgtaatgctc gtgtggtccc
     2221 ggtcgaagac gtagggcgca gcattggcac gacctgctgg cgcaatcgcg acgatcatca
     2281 gcgccaagag caatcgccag cagcgcccgg tcgcgtggtt ccgctgctgg catcctgtcg
     2341 tctgccggcg catcatggcc attatgcttc ctctccggaa caagccgatc acagaccact
     2401 cgaaagcgca tggtgccggc agcaataagg caccctaccg catcagctcg cgcatggctt
     2461 cgtcgaggcc gcgcagcgtc agcggataca tgcggttgcc catcagctgc ttcatgagct
     2521 tgatggaggg cgtccagtcc cagtgctgct cgggcaccgg attgagccag accgcgtgct
     2581 cgtagatgtc ggtgacccgg ctcatccagg cctggccggg ctcctcgttc atgtgctcga
     2641 cggagccgcc cggcaccgtg atctcgtatg ggctcatcga ggcatcaccg acgaatacga
     2701 ccttgtagtc ggccgggtac ttgtgcagca catcccaggt cggcttccgg tccgtccacc
     2761 gccgggcgtt ctccttccag acgaattcgt aaaggcagtt gtggaagtag aagtgctcca
     2821 tgtgcttgaa ctcagcccgg gccgccgaga agagttcctc ggcgagcttc acgtgccagt
     2881 ccatcgagcc gccgatgtcg aagaacatca gcactttcac ggtgttgcgc cgctccggcc
     2941 gcagaatgat atcgagatag cccttgctcg ccgtgccgcg gatcgtgccg tcgaggtcga
     3001 gctcttcatg cgcaccctcg cgcgcgaact tgcgcagccg ccgcatggcg accttgatgt
     3061 tgcgcgtgcc gagctcaacg ctatcgtcga ggtccttgta ttcgcgcttg tcccacacct
     3121 tcatcgcccg gaagttgcgg ttctccttct ggccgatgcg cacgccggcc ggattgtagc
     3181 cctcggcgcc gtagggcgac gttccctgcg tgccgatcca cttcttgccg ccctggtggc
     3241 ggcccttctg ctcggcgagg cgcttcttca gctcttccat gatcttttcc cagccgccga
     3301 gcgcctcgat cttcttcttc tcctcctcgg tgaggtagag cgaggcgacg gcgcgcagcc
     3361 attcctccgg gatcctggcc tcggcggcgt cgctcatcag ctcgaggccc ttgaagacgt
     3421 ggccgaagac ctggtcgaac ttgtcgaggt ggcgctcgtc cttcacgagc gtggcgcggg
     3481 agagatagta gaaatcttcg acgcgtcgat cggcgaggtc ggcctcgagc gcctccatga
     3541 gcgtcaggta ctcgcgcagc gtcacgggga ccttggcggc cttgagctcg aggaagaagg
     3601 aggtgaacat cgggcggtcc cagcaggcgg tggcggcatc tcctgacggc cagttggtgc
     3661 gagaggccgg gctgcgtcaa gtcggaacgc gacgattgca ttgccgcgcg gctctgtggt
     3721 gtggtgagcg cagaccagcc ctgaaggaat agcgaccatg gccctctcgc tcgacggaac
     3781 cctgctactc gccggcgccg gcaacatggg cggcgcgctg ctggcgggct ggctcgagca
     3841 gggactggcg ccctcccgga tcatcgtaca ggatccctcg ccgccgccac gcatgacgga
     3901 gctgctggcg aagcatcgca ttcgcaccga ggccagcatt gaccggatcg atgtgccacc
     3961 cgcggtaatg ctggtcgccg tgaagccgca gatcatggat gaggtgtttc caaagctcgc
     4021 caagctggcc gggcctgcaa cggtcgtgct ctcggttgct gctggcaaga cgatcgccgg
     4081 cttcgagcgc cacctggccc cgggcgccgc cgtcgtccgc tcgatcccga atacgccggc
     4141 ctcggtcggg cgcgggatca cggtcgctgc cgccaacgcg aatgtgacgg ccgcgcagaa
     4201 ggcggcctgc cagaagcttc tcgagagtgt cggcgccgtc gcctgggtcg acgatgaggc
     4261 gctcatcgat cctgtgaccg cggtctcggg ctcgggcccc gcctatgtct tctacctctg
     4321 tgagacgctc gccaaggccg gcgtcgcagc cggcctcgag ccaggtctcg cgcttcaact
     4381 cgcacgctgg acagtcgctg gagccggcga gctgctgcat cgttctgacc tcacgcccga
     4441 cgtccttcgc cagaacgtga cgtcgccgaa cggcacgacg ttcgcggccc tccaggtcct
     4501 gatgggcgag aagggcattc agcccgtcat gacagaggcc gtggcggccg cgacaaagcg
     4561 ctcgcgagag cttgcgaagt aattccgacg agagaagatc ggtcagcatg acttttgctg
     4621 caccgcagca tcaattgctg cgggcaacct gctgaccacg cctaaagtcg aagtgttgat
     4681 ctccgtcaaa ctcccctagc attccaccgg gggggctaca ggcgtcgctc gtcgttctgc
     4741 tccttcacaa caatgaaaaa tgactgcttc gcgaccaggg cgcgaaggga cgcaatcagc
     4801 cggcactccg gccccaaagg agcaggtaat ggcagaaaaa ggcgatcggt actgggcacg
     4861 caccagtacg ctcatgtgga tcatgttgat cctgtggctc ttcttctcgt ttggcgtcca
     4921 catgttcgtg gacaagctca acgcattttc atttctgggc ttcccgcttg ggttctacat
     4981 ggccgcccaa ggctcgctca tcgcattcgt cgtgatgctg ttcgtcttcg ccaagaggca
     5041 ggagacgatc gacaccgagg aaggcgaggc cgaggacgcc tgaggagatt tgagacatgg
     5101 cagcccaaac agcatccgga aagcgcggct ttctcgagaa tctcggcaag atctatggcc
     5161 tctatacggg cggattcttt gccttcgtca tcgtgctcgc agttctcgag cagatgggcc
     5221 tcgatcgcag gtttatcggc tacggcttcg tcttcctgac actcgccgtc tacgccgtta
     5281 tcggcattct gtcgcgcacg atggaagccg gtgaatatta cgtcgccggt cgaacagtgc
     5341 cggccttcta caacggcatg gcgacgggct ccgactggat gtcggcggcc tcgttcatcg
     5401 gcatggccgg caacctgtac ctccagggtt acgacggctt ggccttcgtt ctcggctgga
     5461 cgggtggcta cgtgctggtg gcgatcctga tcgcgccatt cctccggaag ttcggcgcct
     5521 acaccgtgcc tgacttcttc tcggagcgtt acggcggcgg cctcgcccgc ttcctcgccg
     5581 tgatcgtgct gatcgtctgc tccttcacgt atgtggtggc gcagatccgc ggtacgggcc
     5641 tgatcgccca gcagttcctc gacatggact ataatgtcgc ggtctatgtc ggcctcggtg
     5701 gcatcctgct ctgctcgatg ctcggcggca tgaaggcggt tacctggaca caggttgctc
     5761 agtatatcgt gctgatcatc gcctatctga cgccggtcat catcctgtcg gcgaagaagt
     5821 tcaacatccc cgtaccgcag atcacgtatg gctctgcgat ccagcagatc accgagcttg
     5881 agcagagcat gctcaaggcc ggcctcgcgg accccaaggt gttgaagcct cacgcgacac
     5941 cattcctggt gcgcgatgcg atgaactact tcctgctgat cctctgcctg atggtcggta
     6001 cggcgtcgct gccgcacatc ctgatgcgct acttcacgac cccatccgtg agggacgcgc
     6061 gtcggtcggt cgcctggtcg ctgttcttca tcttcctcct ctacttcacg gccccggcct
     6121 acgccgcgtt cgcaaagctc gaggtctaca ccctcatcaa caagaaggtg gcgatcgccg
     6181 atctcgcgaa ggaggcgaac tggctgttca cctacggcaa gtacggcctc gtgaagatct
     6241 gcggcgccaa tgcaacgggt gtggacgctg tcgtggcggc ctgccagaag atcgccgggc
     6301 acggcggttt cctgaggcac caggatctcg ccatccatgc cgactcgatc gtgctttcga
     6361 cgccagagat cgcgaagctg ccctacgtca tcaccggcct cgtcgccgcc ggcggtcttg
     6421 ccgccgcgtt gtcgacagct gatggcttgc tgctcgcaat cgccaacgcg ctcagccacg
     6481 atctctacta caagctgatc gcgccaaact cctcgaccgc gacccggctc atcctatcgc
     6541 gcatcctgct gctcgtcgtg gcggtgatcg cggcctggtg cgccacgtgg ttcccgccgg
     6601 acatcctgat cctcgtgtcc tgggcattca gccttgcggc tgccggattg ttcccggcat
     6661 tggtgctcgg cgtgtggtgg aagggtgcaa cgacgacggg cgccgtgctc ggcatggcgg
     6721 ccgggttcct cgtcaccggc gcgtacatga tcggtacgca ttggtacaag tggccgctgt
     6781 ggtacggggt gtcgaacctc tcggccggca ccttcggtct gatcgtcggc tttgcgacca
     6841 tcatcatcgt cagcctctgc accaagaagc catctgccga ggtccaagcc atgatcgatg
     6901 cgacgcgccg tccgcgcggc gacgcgatca tcaaggacaa ggacgccgcc atagctgcgc
     6961 actgagcagc cgggcgagcc acgacaggaa agggcggagc ttgcgctccg cccttttctc
     7021 ataacagtct cgatatgcga cacggccgcg tctgaggtga atctgctcat gtccccatct
     7081 gaaagcctca tccagcactg gtacttccat ctgccgaacc tggcgctcgc cgcgctgatg
     7141 tacacgttga ttgcggccta tctcatagcg atgttcttca agccggattc ggaagccgtc
     7201 atcctgcgcg tcttcaacag catcgccaat ccgttcttga agctcgtgcg aaccgcgact
     7261 ccggccatcg tgcccgacgg tctgatgatc gtgttcgcga tcgtctggct gatggcgctt
     7321 cgcatgttct ggtttctcac ctgccttgcc gccggcatgc ggctccagat cggaggctag
     7381 tccgtgagcg atgatatgga ctcgcgcgaa ctcaccatgc agcgcgacat tctcatggtc
     7441 gcgaccgcgg gtgcctcact cctgaacggc caggcgttct cgccggtctt cgatcctgtc
     7501 ttcttccttc tgaagccgtt cctcgcagcg gccttctact cgctgccgat ggtggcattc
     7561 tacttgacct cgatcttcat ctcgctcgtc accctcatga tcgccggaat tcctgcggcg
     7621 ctctacgagc gcgcgaaggg cctcaaggag agctcgccga tctcgctcgg catctggctt
     7681 gccgcgacga ttgcgctcgt cagcttgccg tacctcatgc gcgcctgatc ctggaggcgc
     7741 cgaccgcgcg gcgtcgtttg cgcttgacgc cgccggtcct tccccgcaca cctgcggccc
     7801 gtcgacggat caagcacggg aagcgcccat gacagcgatg cacggcctca cggcaatggt
     7861 gaagggcgag atcctttcgc ctttcacgcg ggtgcgccgc ctgctcgacg gcatccctgc
     7921 cggtcacgcc aaacccatcg agatgactgc gggcgatccc aaggagacga tgccgggttt
     7981 cgtcgtcgac cggatcgcgg aggcaaagca gctgctcggc acctatccac atatccgtgg
     8041 ctcagacgag cttcgcaaaa ccatcgccag ctggatcgag cgccgctacg gcctcgccgg
     8101 caagattgat cccgtccgcg aggtgcatcc cgtcaacggc tcgcgcgagg gcctctt
//
LOCUS       DAORNH010000205         8146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6514_length_8146_cov_2.808182, whole genome shotgun sequence.
ACCESSION   DAORNH010000205 DAORNH010000000
VERSION     DAORNH010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6514_length_8146_cov_2.808182"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..964)
                     /locus_tag="PK264_17870"
     CDS             complement(245..964)
                     /locus_tag="PK264_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HRD77777.1"
                     /translation="MRRADRLFEIIQLMRRKPTVRARELAEALEVSERTIYRDIQDLM
                     ASGVPIEGEAGVGYVLKAGFDLPPLMFKEQEIEALVLGARMVEAWADSELALAATDAI
                     AKIEAVIPERLRGYMANTALLAPSSSGVDPIKCDLADLRRAIRNHQKVRFGYTDVLRQ
                     TSERTVRPLSLAFFGPVWLLAAWCELRTDFRTFRLDRIERFAVTAERFRAERGKTLAD
                     FLKRPETWSRSSLGTAGHERH"
     gene            complement(1055..1870)
                     /locus_tag="PK264_17875"
     CDS             complement(1055..1870)
                     /locus_tag="PK264_17875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD77778.1"
                     /translation="MRCHYMSDLHLEAAPFRGALPRGDVLIIAGDLCHARGLDPARAD
                     PYSIAQRGRVFDFIERATRSFAHVLLVAGNHEHYDGVFEDTVPLLRRAFPGVTVLDDE
                     AALIGGVQFFGTTLWSSFEDGSPAALDRVRRRMGEYFFVKRRVTHDDGRTELARFRPE
                     DALEAHRRALRTLQRHLDDASGAMTVIVSHHPPSPSGLNPRHSGNGLDGAYASDLDHL
                     IERITHVPVWVHGHTHIRRTYRIGATLLAANCRGFEGRDATASGFSAKPFFEL"
     gene            complement(2005..2814)
                     /locus_tag="PK264_17880"
     CDS             complement(2005..2814)
                     /locus_tag="PK264_17880"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD77779.1"
                     /translation="MSFAVLLKDWRRRRGLSQLGLSVAADVSARHIAFIETGRARPTR
                     GMILRLSDALDIPRRDRNGLLEAAGFASAYAARDLGAADMLPIRQAVDWMLERHAPFP
                     AIALTRHWVIAALNAPAAAMFGPLGIATSTCLIETFMTPGPLRDAIENWQEVAAHLLA
                     RLKTESRASGGDSVLDGAALRLAAEAGDPIPASNPLPPFLTTRIAVGDLRLALLSTIA
                     QFGSAEDIALAELRIELFFPADEPTRELLSSRSAGLDAEKSRSQSLPQQRR"
     gene            2950..3393
                     /locus_tag="PK264_17885"
     CDS             2950..3393
                     /locus_tag="PK264_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77780.1"
                     /translation="MTSNTLEIPLKAAAALTGAFGIIIALAAYPATSAPARMLVDLVF
                     WPLDGAPDLAAPAARLLAAIAGGVLVGWAVTLWLLVASLSAKRPDVVGSIVKAGIGSW
                     FLVDSAGSIAAGAPINAFLNIGFLVAFLLPVRLARSAGPLAARHD"
     gene            complement(3429..>3706)
                     /locus_tag="PK264_17890"
     CDS             complement(3429..>3706)
                     /locus_tag="PK264_17890"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD77781.1"
                     /translation="LVATGDAHVCQLRWFLLAPEARGQGAGALMIATALGYARAIGCT
                     SVTLWTFAELGAAIALYRRHGFTVVEEKTHEIWGSRRTELRMACALA"
     assembly_gap    3707..3716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3717..3944)
                     /locus_tag="PK264_17895"
     CDS             complement(<3717..3944)
                     /locus_tag="PK264_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD77782.1"
                     /translation="MTPPRQDPPVTLRHELKPGDIGHIITQHGLIYAREYGLDATFEP
                     YVAKPLADFVLGQPTSGRLWIAELGGAFVGSM"
     gene            complement(3941..5053)
                     /locus_tag="PK264_17900"
     CDS             complement(3941..5053)
                     /locus_tag="PK264_17900"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRD77783.1"
                     /translation="MDASGATGSRPRRLLTTFGAIAALVVCAAIATALADPKPDPSRT
                     AAEPIEIKASQIVDFDRSRPGQKRFGRLEYRGGLVLTSSHAGFGGWSGLVVEGDGRRF
                     LAISDAGSWLTGEISYEGSRPKALTGTRIGPILGIKGKSLSRSRDRDAEAVVVAEGSL
                     AKGLVLISFERTHRIGRFPISIDGLGAPLGYIALPPEMRRMSANKGLEAICVLSGGPQ
                     RGALVALSERYPSSTGEHVGWLQSGAAWTGLSIRNIGGFDLVDCVGLADGSLLVLERR
                     FRWSEVLSGVRSRLRQFTSAEIRAGGLMQGTILLEADMGQEIDNLEGLAVHNGPNGET
                     VLTLISDDNFRSWFQRAILLQFALVGETAAVKAPPK"
     gene            complement(5242..7122)
                     /gene="cobT"
                     /locus_tag="PK264_17905"
     CDS             complement(5242..7122)
                     /gene="cobT"
                     /locus_tag="PK264_17905"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HRD77784.1"
                     /translation="MASPPKKNKESPTEPFKRVLGLTCRAISGDDEMQVTYAPGKPEL
                     DGKAMQLPEPSRVPSPREIAVIRGWADSLSLTAAMHDAKVHKRLAPEAGPARAVFEAV
                     ERARVEAIGATRMSGMASNLTAKVEDQYSHGRFADVTDRADAPVEDALSLLVRERLTG
                     MPPPSTAKALVDLWRPLIEEKCAETLSRLQSLAENQSGFARLTRDMLRQLELSDELAD
                     GEKQDGEDENQEDADGGDAEAPESQDSSEGDDQPQEMEAEGQEGDTTDAADAADSEDF
                     DADAEGDEDAETREPWRPNTSVLDDPERFGYRVFNKTFDEEIGAEELSTPDELERLRS
                     FLDKELRNLASAVARLANKLQRKLLAQQNRAWDFDLEEGMLDVARLTRIIMDPMFPLS
                     FKRERDTNFRDTVVTLLLDNSGSMRGRPIMVAACCADILARTLERCGVKVEILGFTTR
                     AWKGGQSREAWLAAGKPPNPGRLNDLRHIVYKTADAPWRRAKRNLALMMREGLLKENI
                     DGEALAWAHKRLLGRPEQRRILMMISDGAPVDDSTLSVNSGSYLETHLRQVIAEIENK
                     SPVELIAIGIGHDVTRYYKRAVTITDPSELAGAMTDQLVELFDETAMAMGHGRGGGRK
                     AG"
     gene            complement(7234..7566)
                     /locus_tag="PK264_17910"
     CDS             complement(7234..7566)
                     /locus_tag="PK264_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77785.1"
                     /translation="MRVRLTSTIVAVALAAASGGASIMPAAAACIVKSEQYAALKLKT
                     KLAEVLKVLGCESRLEKGEQLAPDLKRDVYVWPGECGPLHLVRLTFFNGILYGTEEAK
                     ICLDVKTK"
     gene            complement(7572..>8146)
                     /locus_tag="PK264_17915"
     CDS             complement(7572..>8146)
                     /locus_tag="PK264_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HRD77786.1"
                     /translation="DEYDAGRPDVMFVIQRVLERHGRLTLLDQSRVIRPHPAFRLFAT
                     SNTVGLGDTTGLYHGTQQINQGQMDRWSIVTTLNYLPHDQEVGIVLAKAKTYAKSDAK
                     KKQINNMVRVADLTRAAFINGDLSTVMSPRTVLTWAENSEIFGGDVGFAFRLTFLNKC
                     DELERPLVAEFYQRCFGEELPESAAKVALS"
BASE COUNT         1287 a         2695 c         2773 g         1381 t
ORIGIN      
        1 caccaccccc tcctcccgcc cttgagcgtt gaatgggttg gggtgggggc ggtccgggcg
       61 cggatcatcc cgtgttcatc gccccgagac cggcatgcgc gaccgccagc gaaggacgaa
      121 ttgggggatg cgagcgccgc gtgtgtgacg ttctggtgcg ttgtcaatct tgccccagct
      181 cgcccctcgc cgccggctgc cgcggatccg atcctggacg cgaacagccg cggcacgcgc
      241 agactcagtg cctctcgtga cccgctgtac cgagcgagct acgcgaccag gtctcggggc
      301 gtttcaggaa atcggcgagc gtcttgccgc gctcggcccg gaagcgctcg gccgtgaccg
      361 caaagcgctc gatccgatcg aggcggaagg tgcggaaatc ggtgcgcagc tcgcaccacg
      421 ccgccagcag ccagaccggc ccgaaaaagg cgagcgacag cgggcggaca gtgcgctcgc
      481 tcgtctggcg caggacgtcg gtgtagccga agcggacctt ctgatgattg cggatggcgc
      541 ggcgcagatc agcaagatcg cacttgatgg gatcgacgcc gctcgaggag ggcgcgagca
      601 gcgcggtatt tgccatgtag ccacgaagcc gctccgggat cacggcctcg atcttggcaa
      661 tcgcgtcggt cgcggccagt gcgagctcgg aatcggccca ggcctccacc atgcgcgcgc
      721 cgaggacgag cgcctcgatc tcctgctcct tgaacatcag tggcggcagg tcgaacccgg
      781 ccttgaggac gtagccgacg ccggcctcgc cctcgatcgg cacgccgctc gccatcagat
      841 cctggatgtc gcggtagatc gtgcgctccg agacctcgag cgcctcggca agctcgcgcg
      901 cgcgcaccgt cggcttccgg cgcatgagct ggatgatctc gaacaggcga tcggcacgcc
      961 gcatgggctc ctcctcggcg gtgagcccgt cctctatagc aggtctcctg acagcacgtt
     1021 gtcaggaggc tcggcggcgc gccacggacg gcgatcagag ttcgaagaag ggctttgccg
     1081 agaagccgct ggccgtcgcg tcccggccct cgaagccgcg gcagttggcg gcaagcagcg
     1141 tcgccccgat tcggtaggtg cgacggatgt gcgtgtgtcc gtgcacccag acgggcacgt
     1201 gtgtgatacg ctcgatcagg tgatcgaggt cgctcgcata tgcgccgtcg agcccgttgc
     1261 cgctatggcg cggattgagc ccggaggggc tcggcggatg atgcgagacg atcactgtca
     1321 tcgcaccgga ggcatcgtcg agatggcgtt gcagcgtcct cagtgcgcgc cggtgcgcct
     1381 cgagcgcatc ctcgggcctg aagcgggcga gctcggtgcg accgtcatcg tgcgtgaccc
     1441 tgcgcttcac gaagaagtac tcgcccatgc gacgccggac gcggtcgagc gccgcagggc
     1501 tgccatcctc gaagctcgac cagagcgtcg tgccgaaaaa ctggacgccg ccgatcaggg
     1561 cggcctcgtc gtcgagcacc gtgacgccgg gaaacgcgcg gcgcaggagc ggcaccgtat
     1621 cctcgaacac gccgtcgtag tgctcgtggt tgccggcgac gagaaggaca tgcgcaaagc
     1681 tccgcgtcgc gcgctcgatg aagtcgaaga cgcggcctcg ctgcgcgatg ctgtaggggt
     1741 cggcgcgagc cggatcgagg ccgcgggcgt ggcagaggtc gccggcgatg atgaggacat
     1801 cgccgcgcgg cagggcgcca cggaacgggg ccgcctcgag atggagatcg ctcatgtagt
     1861 ggcagcgcat cgggctctcg cgaatcggat ggggtccgac tatggcgaaa cggcgcgcag
     1921 ctgtcggtta agcggcgtta aggactgcga tcgctcccgc cgccccgatg atccagacgc
     1981 cggcatgccc tgccgcaacg aggttcagcg ccgctgctgt ggcagcgact ggctccggct
     2041 cttctccgca tcaagaccgg ccgatcgcga agagagcagc tcgcgcgtgg gctcgtccgc
     2101 cggaaagaac agctcgatgc ggagctcggc aagggcgatg tcctcggccg agccgaactg
     2161 ggcaatcgtc gacaggagcg ccagcctcag atctccgacg gcgatccgcg tcgtcaggaa
     2221 tggcggcagg ggattgctcg ccgggattgg atcgccggcc tcggctgcga ggcggagcgc
     2281 tgccccatcg aggacagaat cgccaccgct ggcccggctt tcggtcttga gccgagcgag
     2341 caggtgcgcc gcgacctcct gccagttctc gatcgcatct cggagcggcc cgggtgtcat
     2401 gaaggtctcg atgaggcacg tcgacgtcgc gatgccgagc gggccgaaca tggccgccgc
     2461 cggcgcattg agcgctgcaa tgacccaatg gcgggtcagc gcgatcgccg gaaaaggcgc
     2521 atgccgctcg agcatccagt cgacggcctg gcggatcggc agcatgtctg ccgcgccaag
     2581 atcgcgcgcc gcatacgcgc tcgcgaatcc ggccgcctcg aggagcccgt tgcggtcgcg
     2641 cctgggaatg tccagcgcgt cgctcaggcg caagatcatg ccacgcgtcg gtcgggctct
     2701 ccccgtctcg atgaaggcga tgtggcgggc ggaaacgtcg gccgcgaccg agagcccgag
     2761 ctgactcagg ccgcgacggc ggcgccaatc cttgagcaga actgcgaacg acatggagag
     2821 cctccccctt cggcacgccc catttgaacc gaacgagcac cggatttcac ttacccgcaa
     2881 ggtaattgga tcatgtcgcc aggcgcgctt ctctctgtcc atcgacgacg tccacgccgg
     2941 agagacacga tgacgtccaa taccctcgaa atccccttga aagctgcagc cgccctgacc
     3001 ggcgccttcg gcatcatcat cgcactcgcc gcctatcccg cgacaagcgc gcctgcacgt
     3061 atgctcgttg acctggtttt ctggccgctc gacggcgcgc cggacctcgc ggcgccggct
     3121 gctcgtctcc ttgcagccat tgccggcggc gtgctggtcg gctgggccgt gacactgtgg
     3181 ctgctggtcg cgagcctgtc ggcgaagcga ccggatgtcg tcggctcgat cgtgaaagcc
     3241 ggcatcggct cttggttcct tgttgacagc gccggctcga tcgctgcggg cgctccgatc
     3301 aacgcgttcc tcaatattgg ctttctcgtg gccttcctgc tgccggtccg gctcgcccgc
     3361 tcggcggggc ctttggcggc gcgtcacgac tgaatgggat ggcggccaga aggactggag
     3421 tgtaccgctc aggcgagcgc acatgccatt cgaagctcgg tgcgccggct gccccagatc
     3481 tcgtgtgtct tctcttcgac gactgtgaag ccatgacggc gatagagcgc gatcgcggct
     3541 ccgagctccg caaaggtcca gagcgtcacg ctcgtgcagc cgatcgctcg ggcgtagcca
     3601 agcgccgtcg caatcatcag cgcgccggcg ccctggcccc gcgcctccgg cgcgagcagg
     3661 aaccacctga gctgacagac atgtgcgtcg ccggttgcaa caagcgnnnn nnnnnncatc
     3721 gagccgacaa acgcgccgcc gagctccgct atccacagcc gtccgctggt cggctggccg
     3781 agcacgaagt cggcgagtgg ctttgcgaca tagggctcga acgtcgcatc gaggccgtac
     3841 tcgcgcgcat agatgagccc atgctgggtg atgatgtgcc cgatgtcgcc gggtttcagc
     3901 tcgtggcgga gcgtgaccgg tggatcctgg cgcggtggag tcacttgggc ggggccttca
     3961 cggcggccgt ctcgccaacg agcgcgaact gcaggaggat ggcgcgctgg aaccacgacc
     4021 tgaaattgtc gtcggagatc aaggtgagca cggtctcgcc gttgggtccg ttgtgcacgg
     4081 caagcccctc gagattgtcg atctcctggc ccatgtccgc ttccagcagg atcgtgccct
     4141 gcatgaggcc accggctcgg atctcggcag acgtgaactg gcgcaagcgc gatcgaacgc
     4201 ccgagagcac ctcgctccag cggaagcggc gctcgagcac cagcaggctg ccatcggcga
     4261 gccctacaca gtcgaccaga tcgaagccgc cgatgttgcg gatgctgagt ccggtccagg
     4321 ctgcgcccga ctggagccag cccacatgct caccggtcga cgacggatag cgctctgaaa
     4381 gcgcgacgag ggcgccgcgc tgcggcccgc cggagaggac gcaaatcgct tcgagcccct
     4441 tgttggccga catgcgccgc atctcgggcg gcagcgcgat gtagccaagc ggcgcgccga
     4501 ggccatcgat cgagatcggg aagcggccga tccggtgcgt gcgctcgaaa ctgatcagga
     4561 cgaggccttt ggcaagcgag ccctccgcga cgacgaccgc ctcggcatcg cggtcgcggc
     4621 tccgcgagag tgacttaccc ttgatgccga ggatcgggcc gatgcgcgtt cccgtgagcg
     4681 ccttcggcct gctgccttcg tagctgatct cgccggtgag ccagctgccg gcatccgaga
     4741 tggcgaggaa gcgccgccca tcaccttcga cgacgaggcc ggaccagccg ccgaagcccg
     4801 catgagacga ggtgaggacg aggcctcctc gatactcgag gcgtccgaac cgcttctggc
     4861 cggggcgtga ccgatcgaaa tcgacgatct ggctcgcctt gatctcgatc ggctcggctg
     4921 ccgttcgcga cgggtcgggc ttcggatcgg cgagcgcggt cgcgatggcc gcacagacca
     4981 ccagggccgc gatggcgccg aaggtcgtga ggaggcgtct cggtctagat cctgttgcgc
     5041 cgctcgcgtc catctcgacc tgccgaaaga gcccagttca tcagacgccc gaaaagaacg
     5101 ccggaaccat cgccgaatga ttgcggctcc gcgatggcga atgaatgcgc cgctgaccgt
     5161 gcgcagctgc acgtgtcggg gctgcggcgg attggcgccg cgggtcctcg gcgacggtgg
     5221 ccgaggaaca cgcccgccgg ctcagcccgc cttccgccca ccgcctcgac cgtgacccat
     5281 tgccatcgcc gtctcgtcga aaagctcgac gagctgatcc gtcatggcgc cggcgagctc
     5341 ggaggggtcg gtgatcgtca cggcgcgctt gtagtaacgc gtcacgtcgt ggccgatgcc
     5401 gatggcgatc agctcgacgg gcgacttgtt ctcgatctcg gcgatcactt ggcgcaggtg
     5461 cgtctcgagg tagctccccg agttcaccga aagggtcgaa tcgtcgaccg gcgcgccatc
     5521 cgagatcatc atcaggatgc gccgctgctc gggcctgccg agcagccgct tgtgcgccca
     5581 ggcaagagcc tcgccgtcga tgttctcctt gaggagcccc tcgcgcatca taagcgcaag
     5641 gttgcgcttg gcgcgccgcc agggcgcgtc cgccgtcttg tagacgatgt ggcggaggtc
     5701 gttgaggcgg ccagggttcg gcggcttgcc tgcagcgagc caggcctctc gggattgccc
     5761 gcccttccag gcgcgcgtcg tgaagccgag gatctcgacc ttgacgccgc agcgctcgag
     5821 cgtgcgcgcg aggatgtcgg cgcagcaggc cgcgaccatg attggccgcc cgcgcatcga
     5881 gcccgagttg tcgagcagca gagtcacgac ggtatcgcgg aagttcgtat cccgctcgcg
     5941 cttgaacgag agcggaaaca tcgggtccat gataatgcgc gtgagacgcg caacgtcgag
     6001 catgccctcc tcgagatcga agtcccaggc tcggttctgc tgggcgagga gcttcctctg
     6061 cagcttgttg gcgaggcgtg ccaccgcgct cgcgaggttg cgcagctcct tgtcgaggaa
     6121 cgagcgtaac cgctcgagct cgtcgggcgt cgaaagctcc tcggcgccga tctcctcgtc
     6181 gaaggtcttg ttgaagacgc ggtagccgaa gcgctccgga tcatcgagga cgcttgtatt
     6241 gggccgccag ggctcgcgcg tctcggcatc ctcgtcgccc tcggcgtcgg cgtcgaagtc
     6301 ctcgctgtcc gcggcgtcgg cggcgtccgt cgtgtcgcct tcctggcctt ctgcctccat
     6361 ctcctgaggc tggtcgtcgc cctcgctgct gtcctggctt tccggcgcct cggcatcgcc
     6421 cccgtccgcg tcctcctggt tctcgtcctc gccgtcctgc ttctcgccgt cggcgagctc
     6481 atcggaaagc tcgagctggc ggagcatgtc acgcgtgaga cgggcaaagc cagactggtt
     6541 ctcggcgagg ctctgcaggc ggctgagcgt ttcggcacac ttctcctcga tcagcgggcg
     6601 ccagagatcg acaagcgcct tggcggtcga gggtggcggc atgccggtca gccgctcgcg
     6661 cacgagcagg ctcagcgcat cttcaaccgg cgcatcggca cggtccgtca catcggcgaa
     6721 gcggccgtgg ctgtattggt cctcgacctt cgccgtgagg ttgctcgcca tgccggacat
     6781 gcgggtcgcc ccgatcgcct cgacgcgtgc gcgctcgacg gcctcgaata cggcgcgcgc
     6841 gggccctgcc tcgggggcga ggcgcttgtg caccttggca tcgtgcatcg cggctgtgag
     6901 cgaaaggctg tcggcccagc cgcggatgac cgcgatctcg cgaggcgatg gcacccgcga
     6961 aggctcggga agctgcatcg ccttcccatc gagctctggc ttgccgggcg cgtacgtcac
     7021 ctgcatctcg tcgtcgcccg aaatggcgcg acaggtgagc cccagcacgc gcttgaacgg
     7081 ctcggttggg gattccttgt tcttcttggg tgggctggcc attgtcgctc gctgcggtac
     7141 gcgtccaggc tcggaagaat ggtatcaggg catcattcgg ttctgcttgg gagagtttcc
     7201 tctgcgagaa tcgactgcac aacgcaggac aactcacttc gtcttcacgt cgaggcagat
     7261 cttggcttcc tcggtgccgt acaggatgcc gttgaagaag gtcagccgga cgaggtgcag
     7321 ggggccgcac tcgcctggcc agacgtagac gtcgcgcttc aggtccggag cgagctgctc
     7381 gcccttttcc aatcggctct cgcagccgag caccttcaag acttcggcga gcttggtttt
     7441 gagcttcaag gccgcgtact gctcactctt cacgatgcag gctgcggccg ccggcatgat
     7501 actggcaccg cctgacgccg ccgcgagggc aaccgcgacg atcgtcgagg tgagccgaac
     7561 cctcatgccc gtcagctcag cgcgactttg gcagcgctct ccggcagttc ttcgccgaag
     7621 caacgctggt agaactcggc gaccagcggc cgctcgagct cgtcgcactt gttgaggaac
     7681 gtcagccgga aagcgaagcc gacgtcgccg ccgaagatct cggagttctc ggcccaggtg
     7741 agcaccgtgc gcgggctcat cacggtcgag aggtcgccgt tgatgaaggc cgcgcgcgtg
     7801 agatcggcga cgcgcaccat gttgttgatc tgcttcttct tcgcgtcgga cttggcgtac
     7861 gtcttcgcct tggcgagcac gatgccgacc tcctggtcgt gcggcagata gttgagcgtc
     7921 gtgacgatcg accagcggtc catctggccc tggttgatct gctgcgtgcc gtgatagagg
     7981 ccggtcgtgt cgcccaggcc caccgtgttc gatgtggcga acaggcggaa cgccgggtgc
     8041 gggcggatca cgcggctctg gtccagcagg gtcaggcggc cgtggcgctc gagcacgcgc
     8101 tggatcacga acatcacgtc ggggcggccg gcgtcgtatt cgtcga
//
LOCUS       DAORNH010000206         8123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6534_length_8123_cov_2.746901, whole genome shotgun sequence.
ACCESSION   DAORNH010000206 DAORNH010000000
VERSION     DAORNH010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6534_length_8123_cov_2.746901"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="PK264_17920"
     CDS             complement(<1..2086)
                     /locus_tag="PK264_17920"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRD77787.1"
                     /translation="MLPLISSTISFLKRPLGVAALAGLIVTGAALPLAFAQQEAAYNP
                     HPAPGDVLLPLPGGWKIAFTKVPVPGRGFYGGSDRIMEIGGDKQSPNPHEHPRQVRIG
                     ANFFDKSGAGYILVGKYEVTIGQYAALIGDGDIGKGLKLLAESSSIDILTKDAGAAAT
                     LASDMRFLARPIHGLAIDDYWAFLSQLNAWCLTTKEGAACLAAMRKELGSACFFRLPS
                     EPEWEFVARGGIKGMRAAQTLPFPVDDIAARANVGTKKSQPDEIGKRKPHSDLPVYDM
                     FGNVAELMGTPFTLDNGAGAIGSFVLRGGDFRTAASDLRVSYREEEPQFVFRSSAGMF
                     VKRKRPEVGIRLMIGAPVKDPEDRTVTTRIADEFRGSYVPCCGGGGGGGAVADAYGNT
                     LRLARDIGALAIKKNIELSDSVGGPDDPADWFRFAVTQFGTLRMGLQAQDADAIMEVK
                     HESWESPRIFSVKAGRIDAQVLQGLLPGNVRVKVFAADPSKVARWRVIMNFDAYDCCG
                     NRAEEARDLGLLAGRQIDYSDYVGPGDLVDFVKFRTDRVDTISVRLLELTGDANIELF
                     DSEKRLIDKSLNPGNAPERIEFANAPPGTYYIRVFAKDERAASMYKMVIALGNVDTAG
                     DTAKSARDLGTISSDVVTVDESVGAEDRGDVFKFRTTATAVIEIQISKLSSDVTLELL
                     DASERPITRGSRD"
     gene            complement(2104..>3813)
                     /locus_tag="PK264_17925"
     CDS             complement(2104..>3813)
                     /locus_tag="PK264_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD77788.1"
                     /translation="RQRLIQDPAYRLVIPVKAEHRPDEFFAELAKRADVVFVQPDLLA
                     SKTSVRVEPADKAKAQSMEVVPTGPGDPLIVENSGKIPGPAEVVLTLNAAKDLGVEEG
                     AEIKLVFFRSASGRREQQSLTLKVVSVLRGEADGERRLYTVLDVVRDIERFRTGITVS
                     ARNWIGIAAPPEQSFDSVYVVLETAMTEVELLDERIKGGFAEAKVIEPQAFTAATGLA
                     PGAAEQIILLTNTSKPVSGQQLSNTLQRLSVRKFRVIAGAAKLEARLEGVATPVALRP
                     FDPKRFEMKPIEGDAGKWQDWKPGISYMQAERVLIPKGLAEALKAKVGGRLTLNIQPA
                     SAADATKSLAIIVGIDGIVDGDAILIPQALAGMLIRARATPLSFDPALSILVPADVPL
                     RSYRAYGRTIDDVPNIVDYLKDKGDPVHARDQEIKQLQRLDQNLTQMTLIIAAVALIG
                     GAAVLIASFYASVERKKGQLSLLRLLGFSRGSIFSLPVLQSGMLAGLGFLLALGLFLM
                     FSSLINTYYASGLPVRGNICRLTTAHLGIFGLVTLSIAVLASLVAANRTLQIDPAEAL
                     RQE"
     assembly_gap    3814..3902
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4069..4827)
                     /locus_tag="PK264_17930"
     CDS             complement(4069..4827)
                     /locus_tag="PK264_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD77789.1"
                     /translation="MSVLEVKGLKKRREQVGSAFELRVPEFRLEAGRFYGVVGQSGSG
                     KSTLLDMLALVMRPTSAERYMMADPRSGRSSDVSQLWAASDERALAEIRKTMCGYVLQ
                     SGGLIGFLTVRQNLEMPFQLIGRVPDTARIETMAERFGIGTQLDKKPRHLSGGQRQRV
                     AILRALMLEPPLVLADEPTAAVDQVRARQIAAEFRSLAQEAQSTIVMVSHDRELLESV
                     ADRIIELSTEVGANEEAVSTARWSSFTRVLSPVS"
     gene            complement(4907..6946)
                     /locus_tag="PK264_17935"
     CDS             complement(4907..6946)
                     /locus_tag="PK264_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRD77790.1"
                     /translation="MTKTFARARLALAGIAAVALLGSFGRDTVRAQQPDVECKEICRV
                     TKTGLALKVLPRVNVNIYKAKDIQSPTIQSNVDAFVPLLVFERDGIDHSNPTEAKGWY
                     KVGHQVGQPLGFMRATDVLEWRNAVVVSYSHRGTGDSERKPVIMFQKREQLLKVMESH
                     NPLATAANYYKRLDAKDVPEDVITREPDTFVDIKKKFYLLPVLQWLDLTDQGFDDTRI
                     LQIAAAVPGRAAVDPKAACTTQRKDFNDCIQSMGTVGDDQLTIDVVYVIDFSGSMQSY
                     INAVNEATRNSAKVFAAEASAEERIKFGLVSYTDTPASRGDSSGAPVAVNHTAELVTR
                     AALADIIARDGKVIPAGGDIQEETFAGVLEGINARWRSNSVRLIILIGDASGHETSHP
                     QNTTGKDARALRLLATEQNIYIATLYIKNPQQATDWALGQDQFRVLAENPGTTPNFKA
                     VDADPREIENALSEVTAEMVQRIRKLKADRKPAPGASGAPVADAKGSAAGPVGAFRAA
                     LVEYIGRATEPPPDITAWVLDKDMTNHNKRAFDVHVLATRADLDQLTKGVQNLIEAYE
                     LNKTTNEGFFNTLRSMSATLSVDFDVSKSDTLSKSPLIPRWIESLPYKSEIMTMRFQE
                     FEDMNADKRQSQLNRLKSLVQLYRSILERQEAWVKLNETMPHDQHVYPLPLDNLP"
     gene            complement(6981..>8123)
                     /locus_tag="PK264_17940"
     CDS             complement(6981..>8123)
                     /locus_tag="PK264_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77791.1"
                     /translation="PRGSASADDADRCALGDAITCMLVEKRKASAGTKSAAPAGPTGP
                     AAVAPPKVAVPSVPPIPAGPKVADASPPKPVTPPGPGAQPADPKAVDKSAPPSAAASD
                     AGATPAKTKRYIAPAYGVLSSPVAPLKVGGQRQAFVVESVECSDEGNWIVGYLGFLTA
                     TGTGDTGDFVIRQRYAINNYPEEGRLGLGEVKKMPGVTCSADQAECSAPRRRISLVEF
                     KRLKTSAPADPKAAPVPKVADKTKRADDETPRVQSEWRCDAGAGMCFSRERDTWRCDA
                     STGRCMSYDAARWWCVPRREFCSQRVPFGDWGSPLKEGDRVKVGLKPRGGTGGVEKDA
                     NGHPIYDLHVYDMAHPLVTIAADVLTRRCAGHKGPARDWKANLTTN"
BASE COUNT         1280 a         2578 c         2753 g         1423 t
ORIGIN      
        1 cgtcgcggct gccgcgcgtg atcggccgct cggaggcgtc gagcagctcg agcgtgacgt
       61 cggaggagag cttgctgatc tggatttcga tcaccgccgt cgcggtcgtg cggaacttga
      121 agacgtcgcc gcgatcctcg gcgccgacgc tttcgtcgac cgtcacgaca tcggagctga
      181 tcgtgccaag gtcgcgcgcc gacttcgctg tgtcgccagc ggtatcaacg ttgccgagcg
      241 cgatgaccat cttgtacatc gaggccgcgc gctcgtcctt ggcgaagacg cggatgtagt
      301 atgtgcccgg cggcgcattg gcgaactcga tgcgctctgg cgcgttgccg ggattgagcg
      361 acttgtcgat gagccgcttc tcgctgtcga agagctcgat gttggcgtcg cctgtcagct
      421 cgaggaggcg aacgctgatc gtgtcgacgc ggtccgttcg gaacttcacg aagtcgacga
      481 gatcaccggg gccgacataa tccgagtagt cgatctggcg gccggcgagg agacccaagt
      541 cgcgtgcctc ctcggcgcgg ttgccgcagc agtcgtaggc gtcgaagttc atgatgacgc
      601 gccagcgcgc aaccttgctc gggtcggctg caaagacctt cacgcgcacg ttgcccggca
      661 gcagtccttg cagaacctgc gcatcgatgc ggcccgcctt caccgagaag atgcgcgggc
      721 tctcccagct ctcgtgtttc acctccatga tggcgtcggc gtcctgcgcc tgcaggccca
      781 tacgcagcgt gccgaactgg gtgacggcga accggaacca gtcagccgga tcatcagggc
      841 caccgacgct gtcactgagc tcgatattct tcttgatggc gagcgcgccg atgtcccgcg
      901 caaggcggag cgtgttgccg taggcgtcgg cgacagcgcc accgccccct cccccgccgc
      961 agcaggggac gtaggagccg cggaactcgt cggcgatgcg cgtcgtcact gtccggtcct
     1021 cgggatcctt gaccggcgcg ccgatcatca ggcggatgcc gacctcgggc cgcttgcgct
     1081 tgacgaacat gccggcgctg gaccggaaca cgaattgcgg ctcctcctcg cgatagctca
     1141 cacggaggtc gctcgccgcg gtccggaagt caccgccacg caggacgaag ctgccgatcg
     1201 cgcccgcgcc gttgtcgagc gtgaatggcg tccccatcag ctcggcgacg ttgccgaaca
     1261 tgtcgtagac gggcaggtcg gaatgcggtt tgcgcttgcc gatctcgtcg ggctgcgact
     1321 tcttcgtgcc gacgttggcg cgggccgcta tatcgtcgac ggggaacggc agcgtctggg
     1381 cggcgcgcat gcccttgatg ccgccacgag cgacgaactc ccattcgggc tcgctcggca
     1441 agcggaagaa gcaagcagaa ccgagttcct tgcgcatcgc ggcgagacaa gcggcaccct
     1501 ccttcgtcgt caggcaccag gcgttaagct gcgagaggaa ggcccaatag tcgtcgattg
     1561 ccaggccatg gatcggccgc gccagaaatc gcatgtcgga ggcgagcgtt gcggcggctc
     1621 cggcatcctt tgtcagaatg tcgatggaac tcgactctgc gagaagcttg aggcccttgc
     1681 cgatgtcgcc gtcgccgata agtgccgcgt actggccgat cgtcacctcg tacttgccga
     1741 cgaggatgta gccggccccg ctcttgtcga agaaattcgc gccgatccgc acttgacgcg
     1801 ggtgctcgtg cgggttcggg ctctgtttgt cgccgccgat ctccatgatg cggtccgagc
     1861 caccgtagaa gccgcggccg ggcaccggca ccttcgtgaa ggcgatcttc cagccgcccg
     1921 gaagcggcag aagaacatcg ccaggcgcgg gatggggatt gtaagcggcc tcctgctgag
     1981 cgaacgccag cggcagcgcc gcccccgtca cgataaggcc ggcgagggct gcgaccccga
     2041 gaggccgctt caggaacgag atcgtcgacg agatgagcgg cagcatgagg tgacctccaa
     2101 accttattcc tggcgcaaag cttctgcggg atcaatctgc agcgtccgat tggcggcgac
     2161 gaggcttgcc agcaccgcga tcgagagcgt cacgaggccg aaaatgccga gatgcgcggt
     2221 cgtcaaccgg cagatgttgc ccctgaccgg cagtcccgag gcatagtacg tgttgatgag
     2281 gctcgagaac atgaggaaga ggccgagcgc cagcaggaag ccgaggcccg ccagcatgcc
     2341 gctctggagc acagggagcg agaagatcga gccgcgcgag aagccgagga gccgcagcag
     2401 cgagagctgc cccttctttc gctcgacgga ggcatagaag ctcgcgatca gcacggcggc
     2461 gccgccgatc agggccacgg ccgcgatgat gagcgtcatc tgcgtcaggt tctggtcgag
     2521 gcgctggagc tgcttgattt cctggtcgcg cgcatgcacc ggatcgccct tgtccttcag
     2581 gtagtcgacg atgttgggga cgtcgtcgat cgtgcggccg taggcgcggt aggagcgcag
     2641 cggtacgtcg gccggcacaa ggatcgagag ggccggatcg aacgacaagg gcgtcgcgcg
     2701 cgcgcggatc agcatgccgg cgagcgcctg cggaatgagg atcgcatcgc cgtcgacgat
     2761 gccgtcgatg ccgacgatga tcgcgagcga cttcgtcgcg tcggccgcac tcgccggctg
     2821 gatgttgagg gtgagccgcc ccccgacctt cgccttcagc gcctcggcga gacccttcgg
     2881 gatgagcacg cgctcggcct gcatgtagga gatgccgggc ttccagtcct gccacttgcc
     2941 cgcgtcgccc tcgatgggct tcatctcgaa gcgcttcgga tcgaaggggc ggagcgccac
     3001 cggcgtcgcg acgccctcga gccgcgcctc gagcttggcg gcgccggcga tcacgcgaaa
     3061 cttgcggaca gagagacgtt gcagcgtgtt ggagagttgc tggcccgaga cgggcttcga
     3121 cgtgttggtg aggaggatga tctgctcggc cgcacccgga gcaaggccgg tcgctgccgt
     3181 gaaggcctgc ggctcgatca ccttcgcctc ggcaaaaccg cctttgatgc gctcgtcgag
     3241 cagttcgacc tccgtcatcg ccgtctcgag cacgacatag acggaatcga acgactgctc
     3301 cggcggcgcc gcgatgccga tccagttgcg cgccgagacc gtgatgccgg tgcgaaagcg
     3361 ctcgatgtcg cgcacgacgt cgaggacggt atagaggcgg cgctcgccgt cagcctcgcc
     3421 gcgcagcacc gagacgacct tgagcgtgag gctctgctgc tcgcgacgcc ccgaggcgct
     3481 acggaagaag acgagcttga tctcggcgcc ctcctcgacg ccgaggtcct tcgcagcgtt
     3541 gagcgtcagc acgacctcgg ccggcccggg gatcttcccg gaattctcga cgatcagcgg
     3601 atcgccgggt cccgtcggca ccacctccat cgactgggcc ttcgccttgt cagcgggttc
     3661 cacgcgcacg ctcgtcttgg aggccaagag gtcgggctgc acgaatacga cgtcggcgcg
     3721 cttggcgagc tctgcgaaga actcgtccgg gcggtgctcg gccttgacag ggatgacgag
     3781 gcggtaggcg ggatcctgta tgagccgctg gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nngagcgaga gcagcgcctc gtcgcgcagg tcgcgcgcgc cgagaagcgc ggtgaggccc
     3961 gtccggttgg cgagcgtatc gccgttcccg gtcgcatcgc tcgcggcagg cgatggcacg
     4021 gaggttttcg cagtcgagcc ggtcacgcaa atgcccccag gccaaacttc agctgacggg
     4081 tgaaagaact cgggtaaagc tgctccaacg ggccgtcgag acggcctcct cgttggcgcc
     4141 gacttccgtc gagagctcga tgatgcggtc ggcgacgctc tcgagcagct cgcggtcgtg
     4201 cgagaccatg acgatggtcg actgcgcctc ctgtgcgagc gagcggaact cagccgcgat
     4261 ctgacgggcg cgcacctggt cgacggcggc cgtcggttcg tcggcaagga ccagcggcgg
     4321 ctcgagcatc agggcgcgca gaatggcgac gcgctggcgc tggccgcccg agagatggcg
     4381 cggcttcttg tcgagttgcg taccgatgcc gaagcgctca gccatcgtct cgatacgcgc
     4441 cgtatcaggc acacggccga tgagctggaa cggcatctcg aggttctggc ggacggttag
     4501 gaagccgatg aggccgccgg actggagcac gtagccgcac atggtcttgc ggatctcggc
     4561 gagcgcgcgc tcgtcggaag ccgcccagag ctggctgacg tcagagctcc ggccgctcct
     4621 cgggtccgcc atcatgtagc gctccgccga cgtcggccgc atgacgaggg ccagcatgtc
     4681 gagaagggta ctcttccctg agccgctctg gccgacgaca ccgtaaaagc gccccgcctc
     4741 cagcctgaat tccggcacgc gcagctcgaa ggcgctgccg acctgctcgc ggcgcttctt
     4801 gagccccttg acctcgagca cactcatgcg ctgccccctg tggcgctgcc ccccgggtga
     4861 ggaagtgcgg gcgggatcgc tcccgcctgc tcggcttggt cccggctcaa ggcaagttgt
     4921 cgagcggcag cggatagacg tgctggtcgt gcggcatcgt ctcgttgagc ttcacccagg
     4981 cttcctgccg ctcgaggatc gagcggtaga gctggacgag gctcttcagg cggttgagct
     5041 gcgactggcg cttgtcggcg ttcatgtcct cgaactcctg gaagcgcatg gtcatgatct
     5101 cggacttgta aggcaggctc tcgatccagc gcgggatcag cggcgacttc gagagcgtgt
     5161 ccgacttcga gacgtcgaag tcgacggaga gcgtcgccga catcgagcgc agcgtattga
     5221 agaagccttc gttcgtcgtc ttgttgagct cgtaggcctc gatcaggttc tgcacgccct
     5281 tcgtcagctg gtcgaggtcg gcgcgcgtgg ccagaacatg cacgtcgaag gcgcgcttgt
     5341 tgtggttggt catgtccttg tcgagcaccc atgcggtgat atccggcggc ggctcggtgg
     5401 cgcggccgat gtactcgacg agggctgcac ggaaggcgcc gaccggtccc gcggcggagc
     5461 ccttggcatc ggcgacgggc gcgcccgagg caccgggcgc gggcttgcgg tccgccttca
     5521 gcttgcggat gcgctgcacc atctcggccg tcacctcgga gagcgcgttc tcgatctcgc
     5581 gcgggtcggc gtcgacggcc ttgaagttcg gcgtcgttcc cgggttctcg gcaaggacac
     5641 ggaactggtc ctggccgagc gcccagtcgg tcgcctgctg agggtttttg atgtagagcg
     5701 tggcgatata gatgttctgc tcggtggcga gcaggcgcag cgcccgtgca tccttgccgg
     5761 tcgtgttctg cgggtggctc gtctcgtggc cgctcgcatc accgatcagg atgatcaggc
     5821 ggaccgagtt ggagcgccag cgagcgttga tgccctcgag gactccggca aaggtctcct
     5881 cctggatgtc gccgccggcc gggatgactt tgccgtcgcg cgcgatgatg tcggcaagtg
     5941 ccgcacgcgt gacgagctcg gccgtgtgat tgacggcgac gggagcgcca gaactgtcgc
     6001 cgcggcttgc cggcgtgtcg gtgtaggaca ccaggccgaa cttgatgcgc tcctcggcag
     6061 aggcttcggc agcgaacacc ttggccgaat tgcgcgtcgc ctcgttcacc gcgttgatgt
     6121 agctctgcat gctgcccgag aagtcgatga cgtagacgac atcgatcgtc agttggtcgt
     6181 cgccgaccgt tcccatgctc tggatgcagt cgttgaagtc cttgcgctgt gtcgtgcaag
     6241 cggccttcgg gtcgacggcg gcgcggcccg gcacggccgc cgcgatctgg aggatgcgcg
     6301 tgtcatcgaa gccctggtcg gtgaggtcca accactgcaa aacgggcagc aggtagaact
     6361 tcttcttgat gtcgacgaac gtgtctggct cgcgcgtgat cacgtcctcg ggcacgtcct
     6421 tggcgtcgag ccgcttgtag tagttggctg cagtcgcgag cggattgtgc gactccatga
     6481 ccttcagaag ctgctcgcgc ttctggaaca tgatgaccgg cttgcgctcg ctgtcaccgg
     6541 tgccgcgatg cgagtaggag acgacgacgg cattgcgcca ttcgaggacg tcggtcgcgc
     6601 gcatgaagcc gagcggctgg ccgacctgat ggccgacctt gtaccagccc ttggcctcgg
     6661 tgggattcga atggtcgatg ccgtcgcgct cgaagacgag cagcggcacg aaggcgtcga
     6721 cattcgactg gatcgtcggg ctttggatgt ccttcgcctt gtagatgttg acgttgacgc
     6781 gcggcaacac cttgagcgca aggccggtct tggtgacacg gcaaatctcc ttgcattcga
     6841 cgtcaggctg ctgcgcccgc accgtatccc ggccgaacga gccgagtagc gcgacggcgg
     6901 caatgccggc gagcgccaag cgtgctcttg caaaagtctt tgtcatatca acgcctccga
     6961 ggcctttctt cagttgtagc tcagtttgtc gtgaggttcg ccttccagtc gcgcgcgggg
     7021 cccttgtggc ccgcgcagcg gcgcgtcagc acgtccgctg cgatcgtgac gagcggatgt
     7081 gccatatcgt atacgtgcaa gtcgtagatc ggatggccgt tcgcatcctt ttcgacgccg
     7141 ccagtgccgc cgcgcggctt caggcccacc ttgacccgat cgccttcctt caatgggctt
     7201 ccccagtcgc cgaagggcac gcgctgcgag cagaactcgc gccgcggcac gcaccaccag
     7261 cgagcggcat cgtagctcat gcagcgaccg gtcgaggcat cgcagcgcca tgtgtcgcgt
     7321 tcacggctga agcacatgcc ggcgccggcg tcacagcgcc attccgattg cacgcgcggc
     7381 gtctcgtcgt cggcgcgctt cgtcttgtcg gcaaccttcg gcacgggggc agccttcgga
     7441 tcagcgggcg ccgacgtctt cagacgcttg aactcgacga gcgagatgcg ccgccgcggc
     7501 gccgagcact cggcctgatc ggccgagcat gtcaccccgg gcatcttctt cacttcgccg
     7561 agcccgagcc gcccctcctc gggatagttg ttgatggcgt aacgctgacg gatcacgaaa
     7621 tcgccggtgt cgccggtacc tgtcgccgtg aggaagccga ggtagccgac gatccagttg
     7681 ccctcgtcgc tgcactcgac cgactcgacg acaaaggcct gccgctggcc gccgaccttc
     7741 aatggggcca cgggcgaact caggacgcca taggccggcg cgatgtatcg cttagtcttc
     7801 gcgggtgtcg caccggcatc ggacgccgcg gccgacggcg gcgcggattt gtcgacggcc
     7861 ttcgggtcgg caggctgggc accaggaccg ggcggcgtca cgggtttggg cgggctcgcg
     7921 tcagccacct tcggtccggc tggaattggc ggcactgatg gcacagcaac ctttggtggt
     7981 gcgaccgcgg cgggtccggt cggcccggcg ggtgcggcag atttcgtccc ggccgacgcc
     8041 ttgcgcttct cgaccagcat gcaggtgatc gcatcgccga gcgcgcagcg gtcggcatcg
     8101 tctgccgacg cactgccgcg cgg
//
LOCUS       DAORNH010000207         8113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6548_length_8113_cov_2.777736, whole genome shotgun sequence.
ACCESSION   DAORNH010000207 DAORNH010000000
VERSION     DAORNH010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6548_length_8113_cov_2.777736"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PK264_17945"
     CDS             complement(<1..1038)
                     /locus_tag="PK264_17945"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HRD77792.1"
                     /translation="MSHRPPRVAIIGVWLESNRAAPVTGESQFRGFYWLEGEAILEAA
                     RAKNPSILGEAAAFVKTMDATGLWEPVPILIAGAQPWGPVDGRLMDRFLDLIGDGLRQ
                     AGPLDAVYVANHGAMIATNRDDPDGEVIALAREIAGPAARIVVTLDLHGNISDRMVSE
                     CDLIVGYRTNPHVDMKERGEEAALSLRLMLAGFADPKSAFIRLPLTPASVNLLTASGP
                     YGDVIDFGTRRRAEMSGDILNVSIFGGFVFSDTTKNGIAIIVTARDDLTRARALATEI
                     AERCWGQRERFRKVLTPLPEAVRRALAAMRTAIGVASVAWHDRSRRPRPRHRVARRRR
                     RAVRRRRCGRAA"
     gene            complement(1169..1546)
                     /locus_tag="PK264_17950"
     CDS             complement(1169..1546)
                     /locus_tag="PK264_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD77793.1"
                     /translation="MFSHVIVGSNDVAASQKFYDAVLGALGIPAGAADPKGRVFYRTK
                     TGLFGITKPIDGQAACHANGGTIGFAAETPEKVKAFHAAGVAAGGKSIEDPPGWREMG
                     PFKLYLAYLRDPAGNKICAMCRG"
     gene            complement(1686..2198)
                     /locus_tag="PK264_17955"
     CDS             complement(1686..2198)
                     /locus_tag="PK264_17955"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRD77794.1"
                     /translation="MTFDWLFEVLKTHPSLIPVTTFVVALGEAIVFTSPLVPATVLVI
                     GLGALQQAAGGSFAAIAIAAIAGTFLGDVASYAIGRRYRGSIGTWWPLRNNPGWLPAA
                     IEFMRNWGWAGLIGSKFLGPVRWFGPAICGVLVMPVAPFLLVTILASVIWALVVLGPP
                     YYGTHVLFGG"
     gene            complement(2800..2875)
                     /locus_tag="PK264_17960"
     tRNA            complement(2800..2875)
                     /locus_tag="PK264_17960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2840..2842),aa:Ala,seq:ggc)
     gene            3143..3486
                     /locus_tag="PK264_17965"
                     /pseudo
     CDS             3143..3486
                     /locus_tag="PK264_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531141.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
     assembly_gap    3487..3526
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3927..>4912
                     /locus_tag="PK264_17970"
     CDS             3927..>4912
                     /locus_tag="PK264_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD77795.1"
                     /translation="MPEVKVIERIEEVPAADWNRLAVPDGAPANPFVMHEFLAALERS
                     GSVGPRTGWAPRHLVLRETGALQGAMPLYLKTHSQGEYVFDHGWAEAFQRAGGQYYPK
                     LQAAVPFTPVPGPRLLVGAGEGAAERERLLAKAAIEVGRRMGVSSLHVTFLDEAQAER
                     IAGEGYLLRTGRQFHWQNRDYRSFDDFLASLASRKRKAVRKERAEAIAGGISIEHLAD
                     GDITEAHWDAFFDFYMDTGGRKWGRPYLNRRFFSELGASMADRCLLVMAKRDGRYIAG
                     ALNLVGGDCLYGRYWGAIEHHPCLHFEVCYYQAIEWAIGRRLPRVEAGAQGD"
     assembly_gap    4913..5020
                     /estimated_length=108
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5260..>5913
                     /locus_tag="PK264_17975"
     CDS             5260..>5913
                     /locus_tag="PK264_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD77796.1"
                     /translation="MTPASSGQDVAAIAEARLSSKPLARTSWRWARGGLSAVALALVV
                     LTAAAGGTSLARSGPESIAPLAEKLIDAVVNISTTQTVKGASGAPLPNVPKGSPFEEL
                     FDDFFKKGKGRPERKQSSLGSGFVIDGKEGLIVTNHHVIDGADEIIINFNDGSKLKVD
                     RVIGKDTKTDLALLKVTPKRPLASVPWGPSAKVRVGDWVVAIGNPFGLGGSVTAGVLS
                     "
     assembly_gap    5914..5959
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5960..6773
                     /locus_tag="PK264_17980"
     CDS             <5960..6773
                     /locus_tag="PK264_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRD77797.1"
                     /translation="DAAINKGNSGGPLFNMSGEVIGVNTAIISPSGGSIGIGFAVPSE
                     TAIFVVDQLRRHGEIRRGWLGVNVQSLNEGLAEEFGSPPGLGALVTRVMPGSPAAAAG
                     LKSRDIIVRFDGKDISTKSGLPRAVAQTEVGREVEVVVLRDGQRMSFKVKVALLVEDA
                     PASKGPPRAPELIVKGLKLAPMTEELRKTHGLPERLKGVVVIEVEAASLAAQRGFMIG
                     DVIFEVAGEAITTLQDVAKGYDRVRTAKRPNVLIKVENAKGEIRSIAMPVDQ"
     gene            7001..7885
                     /locus_tag="PK264_17985"
     CDS             7001..7885
                     /locus_tag="PK264_17985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HRD77798.1"
                     /translation="MVTIRAFVPEDAFTAETLGTERAGHGVVIDHDGLVLTIGYLVTE
                     AETVWINFANGSAVQGHVLGYDQETGFGLLRALGRVDVPAVSLGSSARAEIGERVVVG
                     GLGRSDSVAARIVAKQEFAGYWEYVLDEAIFTAPSHPNWGGTAVIGPSGDLIGIGSLQ
                     VQHVARDKSAQNINMIVPIDLLKPILADMVSTGRANRPPRPWLGVYATEMNNRLVVAG
                     VSQKGPAKSAGITAGDVLLEVAGESVRDLARMFRRIWSLGDAGVEVPMRVSRDGRTLE
                     VRVKSGDRRQFLRRPVLH"
BASE COUNT         1411 a         2619 c         2615 g         1274 t
ORIGIN      
        1 cgcagcgcga ccgcagcgac gacgacgtac agcgcgacgg cgccgccgcg caacgcggtg
       61 tcgaggacga ggacggcggg accggtcatg ccacgcgacg gatgctacac caatcgccgt
      121 gcgcatcgct gcgagcgcgc gccgcaccgc ctcgggaagc ggcgtcaaca ccttgcgaaa
      181 gcgctcgcgc tgaccccagc agcgctccgc gatctctgtc gcgagcgcgc gtgcccgggt
      241 gagatcgtcg cgtgccgtca cgatgatggc gatgccgttc ttcgtcgtgt ccgagaagac
      301 gaagccgccg aagatcgaca cgttcaggat atcgccgctc atctcggcgc gccgccgcgt
      361 accgaaatcg atgacatctc cgtagggccc gctcgcggtc agcaggttca ccgaggccgg
      421 cgtgagcggc agccggatga aggccgattt cgggtcggcg aacccggcga gcataagccg
      481 cagcgacagt gccgcctcct cgccccgctc cttcatgtcg acatgcgggt tggtgcgata
      541 gccgacgatc aaatcgcatt cgctcaccat gcgatccgag atgttgccgt gcagatcgag
      601 cgtgacgacg atgcgggcgg ccgggcctgc gatctcgcgg gcgagcgcga tcacttcgcc
      661 gtccggatca tcccgattgg ttgcgatcat tgcgccgtgg ttggcgacat agacggcatc
      721 gagcgggccc gcctgccgga gcccatcgcc gatcagatcg aggaagcgat ccatgaggcg
      781 gccgtcaacc ggcccccagg gctgcgcgcc ggcgatgagg atcggcacgg gctcccatag
      841 gccagtcgca tccatggtct tcacgaatgc tgccgcctcg ccgaggatcg acgggttctt
      901 cgcgcgcgct gcctcgagga tcgcctcgcc ctcgagccag tagaagccgc ggaactggct
      961 ctcgcccgtc acaggtgcag cacgattgct ctcgagccag acgccgatga tggcgacgcg
     1021 cggcgggcga tgtgacatgg ctcgttctcc cacgaggtcg ttcgtcggca gtcggcgatc
     1081 cgaggcgagg tcaccgccga gcgtatgacg aaaacggcgc agggccagcc gaccccgcgc
     1141 cgagatgcac agcacgcgcg atcacgtatt agccgcggca catcgcgcaa atcttgttgc
     1201 cggccggatc acgcaggtag gcaagataaa gcttgaaggg gcccatctcg cgccagcccg
     1261 gcgggtcctc gatcgattta ccgccggctg cgacgcccgc cgcatggaac gccttgacct
     1321 tctccggcgt ctccgcggca aatccaattg tgccgccgtt ggcgtggcag gcggcctggc
     1381 cgtctatcgg cttggtgata ccgaagaggc cggtcttggt gcgataaaat acgcggccct
     1441 tgggatcggc cgcaccggcg ggtataccga gcgctccaag cacggcgtcg tagaatttct
     1501 gcgaggcggc aacgtcgttc gatccgacga tgacgtgaga gaacatgggg actcctcaag
     1561 ggctaactcg atccgcgtgg ccgccgcggc cggcgccagc ggcccgaact tgagcacgcg
     1621 ccgcccccgc tgaccagtca cgatcatgac agggcgtcat gcgagcagca ttgcccggcc
     1681 atcggtcagc ctccgaagag gacgtgcgtc ccgtagtacg gcggtccgag caccacgagt
     1741 gcccagatga cggatgccag gatcgtcacc agcagaaacg gcgccaccgg catcaccaga
     1801 acgccgcaga tcgccggacc gaaccagcgg actggcccga ggaacttcga gccgatgagg
     1861 cccgcccagc cccagttccg catgaactcg atcgccgccg gcagccaacc cgggttgttt
     1921 cgcaaaggcc accaggtgcc gatgctgccc ctatagcgcc ggccgatcgc ataggaggcg
     1981 acgtcgccga ggaacgttcc ggcgatggcc gcaatcgcga tcgccgcgaa tgagccgccc
     2041 gccgcctgct gcagcgcgcc gaggccgatc accagaaccg tggccggtac cagcggcgag
     2101 gtgaacacga tcgcctcgcc gagtgccacg acaaaggtcg tgaccgggat gagcgacgga
     2161 tgggtcttca gaacctcgaa cagccaatcg aacgtcatgt ccctcccgtc acgcgcgagc
     2221 gcgagatcgg cgcccatgcc ggccacaatg ggatcccggt gtcaagggat tctgcgctct
     2281 ctggcggcgt ggctgaagct cgccagctcc atcgagaccc gcatcagccg ccgcagtcgc
     2341 tggatcacaa gctactcggc gacgcgcttc tgtggcccgc aataacttaa caagagaccg
     2401 cagccatcgc tgacatcata aactgcaagc cgaccgccaa ccgctcgttc gtcaatgacc
     2461 caggtccgga ccaggagggt aaatggccgg cctcatcgtc atccgcgtcc gcacagtgaa
     2521 ggccgccaac ggaggagcga gccaatggca ccgtgcaccc gcgcattgct gacctcgctc
     2581 tgtccgttcc ccctcactgg gtgatcgacg ggcagttcgg cgacgtcatc gacgccctga
     2641 tagccaggtg tcccagacgc gaccatgcaa ttggccctgc ccactgcatt cagcgaatag
     2701 ccacgagttc gcgtgcgaca tctcgtcccg aaatgggtag cccggcggta gccgcgtctc
     2761 gccgtcggat ttcacaagtc tcaaaatcac tgatttcgtt ggtggagccg agggggatcg
     2821 aacccctgac ctcgtgaatg ccattcacgc gctctcccag ctgagctacg gcccctgacc
     2881 attgggatgc ggccgcgtat cagcggccgg cgcaccctcc taatcaagtc gaagcgaatc
     2941 cgcaagcgct agtcgtcgac gactgtacaa ttgccgttgc gcgacctttt tgcgacgttg
     3001 cggtcagggc cttgaaatcc gatttggccc tcccaaatca agcctcgtgc aggcccagac
     3061 gggatgcaca ttaggactgc actgccgagc tgccgggccc tcgggccggt tcaatcacag
     3121 tcgcttctca ggaggactga ctatgcgtgc attcgacttc tcgcccctct atcgctcgac
     3181 cgtcggcttc gaccgactgt tcaacatgct ggatcaggtc tccggcgtcg acaacgatgg
     3241 gagcgcctac cccccctaca atatcgagcg catcggcgaa aacgcctatc gcatctccat
     3301 ggccgtcgcc ggcttcaccg cggccgagct taaggccgag gtgcgtgagg gtgtcctcac
     3361 catcaccggc gcgaaagccg ccgaggacaa gggccgcgcc tttttgcacc gcggcatcgc
     3421 cgcgcgaagc ttcgagcgcc gcttccagct cgcagaccac gtcgaaatca agggcgccga
     3481 tctggcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggct gaagccgcgt
     3541 tcgatcgcca tcggctcggc gccgtctcag cgcatcgagg cgcagatcga ggcgaaggcc
     3601 gccgacgtca aggcggcctg atctcggcgt caatcagccg cgaaggggag cgtcctcggc
     3661 gctccccgtc tcgcatggcg gggacattcc attctccgaa gtggttcccc tgtcgtagcg
     3721 cgaaagcgcg aagagccggg gcctttacct gcgaaggtgt cagggcccca gctctcgctc
     3781 tgctcggccg gggaaccatc acggggcaat tggttggcgc cgcccagcac tgaactgctc
     3841 cgtcgcactt tgctgcaaag ctcgtggtct cttgacaagc gcctgtcgta cttgccggat
     3901 tgcggcgcga cggcaggagc ggcggcatgc cagaagtcaa ggtcatcgag cggatcgagg
     3961 aggtcccggc agcggactgg aaccggctcg ccgtcccaga cggcgcgccg gccaacccat
     4021 tcgtcatgca cgagttcctc gccgcgctcg agcgatcggg ctcggtcggg ccgcggaccg
     4081 gatgggcgcc tcgccacctc gtattgcgcg agacgggagc cctgcagggg gcgatgccgc
     4141 tctatctcaa gacccacagc cagggcgagt atgtgttcga tcacggctgg gccgaggcct
     4201 tccagcgtgc cggaggacag tactacccga agctccaggc cgcggttccc ttcacgccgg
     4261 tgcccgggcc gcggcttctc gtcggcgccg gcgagggagc agcagagcgt gagcggctgc
     4321 tcgccaaggc ggcgatcgag gtcggccggc ggatgggggt atcgtcactc catgtcacgt
     4381 tcctcgacga ggcccaagcc gaacggatcg cgggcgaggg ctatctgttg cgcaccggcc
     4441 gccagttcca ctggcagaac cgcgactatc gaagcttcga tgattttctg gcctccctcg
     4501 catcgaggaa gcgcaaggcc gtccgcaagg agcgcgccga ggccatcgcc ggcgggatct
     4561 cgatcgagca cctcgcggat ggcgacatca cggaggcaca ctgggacgca ttcttcgatt
     4621 tctacatgga cacgggtggg cgcaagtggg ggcgcccgta tctcaaccga cgcttcttct
     4681 ccgagctcgg cgcaagcatg gccgatcgtt gcctgctcgt catggcgaag cgcgacggcc
     4741 gctacatcgc gggtgccttg aacctcgtcg gtggcgattg cctctatggc cgctactggg
     4801 gcgcgatcga gcatcacccc tgcctgcatt tcgaagtctg ctactatcag gcaatcgaat
     4861 gggcgatcgg ccggcgcctg cctcgcgtcg aggccggcgc gcagggtgat cannnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cacgtggccg aggagcggca
     5041 gatgctcgcc gagtacctgc cttttcgaaa ggtcgtcgcg gccgaggatg cctaagcgtc
     5101 cggcgcgagc attgcgtcga attctgggcg atgattacag gatcgtagct tgacatgcgg
     5161 ccttgcgccc aagaagcatg tgacgagaga cagcgcttgg cggcacgccc cgagccggcg
     5221 acgaccacgc cataacgcga ttggatcgag gagagtgcaa tgacgcccgc gagttcaggc
     5281 caggatgtgg ccgcgatcgc cgaggctcgc ctgagcagca agccattggc gaggacgagt
     5341 tggcgatggg cgcgcggcgg actgtccgcc gtggcgctgg cgctcgtggt gctgacggcg
     5401 gcggcgggcg gcacgagtct cgcgcggtca gggcctgaga gcatcgcgcc gctcgccgag
     5461 aagctcatcg acgccgtggt gaacatctcg acgacgcaga cggtgaaggg cgcgtcgggt
     5521 gcaccgctgc cgaacgtgcc gaagggctcg ccgttcgagg agctgttcga cgacttcttc
     5581 aagaagggca agggccggcc ggaacgcaag cagagttcac tcggttccgg cttcgtaatc
     5641 gacggcaagg aaggcctcat cgtcacaaat caccatgtga tcgacggcgc cgacgagatc
     5701 atcatcaact tcaacgacgg ctccaagctg aaggtggaca gggtcatcgg caaggacacg
     5761 aagacggatc tcgcactcct caaggtgacg ccaaaacggc cgctggcgtc tgtgccgtgg
     5821 gggccgtccg caaaagtacg cgtcggcgac tgggttgtgg cgatcggcaa tccgttcggc
     5881 ctcggcggct cggtcactgc cggcgtcctc tccnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnc gacgcggcga tcaacaaggg caactcgggc ggaccgctct
     6001 tcaacatgag cggggaagtg atcggcgtaa acacggcgat catctcgcct tccggtggct
     6061 cgatcggcat cggcttcgcg gtgccctccg aaacggcgat tttcgtcgtc gaccagctcc
     6121 gtcgacacgg cgagatccgc cgtggctggc tcggcgtgaa cgtccagtcg ctgaacgagg
     6181 gcctcgccga ggagttcggc tcgccgccgg gcctcggcgc gctcgtcacg cgcgtgatgc
     6241 cgggcagccc cgccgccgcc gccggcctca agtcgcgcga catcatcgtc cgcttcgacg
     6301 gcaaggacat ctcgacgaag agcgggttgc cgcgcgccgt agcccagacc gaggtcgggc
     6361 gcgaggtcga ggtcgtcgtg ctgcgcgatg gccagcgcat gagcttcaag gtcaaggtgg
     6421 cgctcctcgt ggaggatgcc ccggcgagca aggggccgcc gcgcgcgccc gagctgatcg
     6481 tcaagggcct gaagctcgcg ccgatgacag aggagctgcg caagacgcac ggtctcccgg
     6541 aacgcctcaa gggtgtcgtc gtgatcgagg tcgaagcggc gagcctcgcc gcgcagcggg
     6601 gcttcatgat cggcgacgta atcttcgaag tggcgggaga ggcgatcaca acgcttcagg
     6661 atgtcgcaaa gggctacgac cgcgtgcgca cggccaagcg tccgaatgtg ctgatcaagg
     6721 tcgagaatgc aaagggcgag atcagatcga ttgccatgcc ggtcgatcag tagcagtctc
     6781 cccgcggcga cgagacgtgg atacacgatg gtgccgatgg ccgccatgtt tgcggccgtc
     6841 gctcgccatc gcagtcactc ggcatgaggg cggtcatgga gccacccgcg cgaggtggcg
     6901 aagtccgtat ttcgaaagcc cgcgccacga cagagagggt cgcgccgtgc aaccaaatcc
     6961 gcacgattat tcctacgacc tcgatcgggt cctcgcctcg atggtgacga tccgcgcctt
     7021 cgtgcccgag gatgcgttca cggccgagac actcggcacc gaacgcgccg gacacggcgt
     7081 cgtgatcgac catgatgggc tcgtgctgac catcggctat ctcgtcaccg aagccgagac
     7141 ggtctggatc aatttcgcca acggcagcgc cgtccagggt cacgtgctcg gctacgacca
     7201 ggaaaccggg ttcggcctcc tgcgcgcact tggccgggtg gacgtgccgg cggtatctct
     7261 cggcagctca gcgcgggccg agatcggtga gcgcgtcgtg gtgggcgggc tcggccgctc
     7321 ggattcggtc gccgcccgca tcgtcgccaa gcaggagttc gccggctact gggagtatgt
     7381 cctcgacgaa gcgattttca ctgcgccatc gcatccgaac tggggcggca ccgccgtgat
     7441 cgggccctcg ggtgacctca tcggcatcgg ctccctgcag gtgcagcacg tcgcgcgcga
     7501 caagtcggcg cagaacatca acatgatcgt gccgatcgac ctcctgaagc cgatcctcgc
     7561 cgacatggtg tcgacgggtc gtgccaaccg gccgccgcgt ccctggctcg gcgtctatgc
     7621 gaccgagatg aacaaccgcc tcgtcgtcgc gggcgtgtcg cagaaggggc cggcgaaaag
     7681 cgccggcatc acggccggcg acgtgctgct cgaagtcgcc ggtgaaagcg tccgcgatct
     7741 tgcccgcatg ttccgccgca tctggtcgct cggggatgcc ggagtcgagg tgccgatgcg
     7801 cgtttcccgt gatgggcgca ccttggaagt ccgcgtgaag tcgggcgacc ggagacagtt
     7861 cctgcggcgc ccggtgctgc attgacgctc aaacccaagg tatcgtgccg acgagccgcg
     7921 ggacgtgccg acatggaggg gacgagatga cgagcccgac gctggagacg ctcaaggcga
     7981 agctcttcac ctccgtgctg tcggacagcc tcgatgccgc agggttcatg agccaggcgc
     8041 tacctgcacg catccgcccg ctcgacgaca cgctcgtgat cgtggcgacg gagttcggcc
     8101 gcacggtcta cag
//
LOCUS       DAORNH010000208         8090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6567_length_8090_cov_2.807592, whole genome shotgun sequence.
ACCESSION   DAORNH010000208 DAORNH010000000
VERSION     DAORNH010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_8090_cov_2.807592"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..820
                     /locus_tag="PK264_17990"
     CDS             296..820
                     /locus_tag="PK264_17990"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HRD77799.1"
                     /translation="MHIIAALVAAAGVVLFILYRMQQAATAARDVAEAASDLSGLFRS
                     WNWRRRANVNPLDAIEDPREAAAALMVALAQYDGAVTEAESRVIVGEMTKHFGATASE
                     AGELLARGRWLAPERADVGELMRRLKRVIEAKCGPKERRELIAMLRAVANASGTPDET
                     LEHDIANLEKSLAA"
     gene            complement(817..1206)
                     /locus_tag="PK264_17995"
     CDS             complement(817..1206)
                     /locus_tag="PK264_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77800.1"
                     /translation="MFAAGQVWTYRTPPGSEASRMVVGAVLAFAEPPAIVCCAVLGAP
                     QRMPDGTVSRITIPFLPMTQPALAASVIALEGSGEPPAAFAAQFAAWRSEARGLAYFT
                     VPFEGHLDQMIARQMAEIAGVDPGGAG"
     gene            complement(1245..2054)
                     /locus_tag="PK264_18000"
     CDS             complement(1245..2054)
                     /locus_tag="PK264_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD77801.1"
                     /translation="MRLKDKVAVIIGAGQSPGEGMGNGRATAMLFAREGAKVLAVDRD
                     LASAEETAVLANKDAKGSTLAFKGDVVREADMKAAIEEAVKRFGRIDCLHYNVGVSLG
                     GGDASPTEITEEAFDRVNAINLRGFVMAVKHALPVFRRQQSGVILSISSVAALSATYK
                     TVAYKTTKAGMIAYTEQIARQNAEFGIRANVILPGLMDTPMAVDTRARVWGRTRADVA
                     AERDAKVPLKRKMGSAWDVAYACLFLASDEAKFITGVALPVDGGALLRASD"
     gene            complement(2093..2644)
                     /locus_tag="PK264_18005"
     CDS             complement(2093..2644)
                     /locus_tag="PK264_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD77802.1"
                     /translation="MARVPYLEKSDLAAADHDLLARPITLYRALVNSPGGARAFHGLG
                     HYIRYKSKLDPRLRELAILQVGWLARSPYEWSHHVKLGYDFGVTDDDIKALIADTDGK
                     PNPLEPLVKTALRAAREVTRDGAVSKATFAELRKSLSNESVTDLVITISFYNAVVRLL
                     GSLEIDVEPEYQPYLDRYPLPRS"
     gene            complement(2748..3095)
                     /locus_tag="PK264_18010"
     CDS             complement(2748..3095)
                     /locus_tag="PK264_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77803.1"
                     /translation="MRKSFGRSPVIAIAAIGFGVSLSACSTAMLESVPAPRGPVCDVA
                     SATVTMANRDVAAYFAKVRLAEQIGEVKSELSAAGVMLARAERQVQCVPYRLFGPDTG
                     LYSCTASANVCSR"
     gene            complement(3307..3486)
                     /locus_tag="PK264_18015"
     CDS             complement(3307..3486)
                     /locus_tag="PK264_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HRD77804.1"
                     /translation="MQRLLAIFWRNESGATAIEYGLIAAFIAVALVTTLVNVGTEVKG
                     PFVDAEAGLKKRPAI"
     gene            3748..5382
                     /locus_tag="PK264_18020"
     CDS             3748..5382
                     /locus_tag="PK264_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD77805.1"
                     /translation="METYDYVIIGAGSAGSVLAARLSEDAGSNVCVLEAGPKDWHPYI
                     HIPAGFIKTFYDKRVNWLYSMEPSEWTGGRRIHAPRGKTLGGSSSINGHIYNRGQRLD
                     FDTWSQFGNRGWGYADVLPYFRRMERREGPSDPTYRGKDGALTVSNIDWTHPLCEAFI
                     EGAVSLGIPRNPDYNGGIQEGVSYAQRTIERGWRMSAAKAFLKPAMKRPNLKVVTHAH
                     ATSIVLEGKRAVGVRYLKGGRNGTQVEIRARREVILSGGAYNSPQLLQLSGIGPAEQL
                     ASHGITVHHALPGVGENLRDHYAPRFVARVKNIETINERVRGLKLVGEAMKWAAGRPS
                     ILGLSPTLIYCFWRSHPDIANSDIQLTFTPASYREGVQGELEREPGMTVASWQQRPDS
                     RGYVRLRSADPFAPPIIQPNYLAEEGDRRVLLAAMRLARRLLASKPLEHYTAYEDFPG
                     PKVQSDDELLGAAKERGTTTFHPAGTCRMGPGTDKLAVVDDQLRVRGLEGLRVIDASI
                     MPTMLSANLNAATMMIAEKASDIIRGRAPPEPLVNA"
     gene            5379..6236
                     /gene="rlmJ"
                     /locus_tag="PK264_18025"
     CDS             5379..6236
                     /gene="rlmJ"
                     /locus_tag="PK264_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HRD77806.1"
                     /translation="MNYRHAFHAGNFADVVKHVILSRILLHLGRKPAPFRVVDTHAGA
                     GRYSLAGSEAARSGEWQGGIGRLIGSGAAPLPSDIQHLMSPYLDIVRRANSDGGFSVY
                     PGSPMIAAAMLRPDDHLVVNELQPDEHAALASAMAGDRRVTTLSIDGYAAVRATLPPK
                     ERRGLVLIDPPFEKPGELDRLVGAIKDGVKRFATGTFMIWYPIKDLRSAERLHVALAA
                     TGIAKLLAIDLTVRHPVRNGRVLAGAGQVILNPPFTLAGEMEAILPVLATRFAVAPGA
                     SASVKAITS"
     gene            6541..6759
                     /locus_tag="PK264_18030"
     CDS             6541..6759
                     /locus_tag="PK264_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRD77807.1"
                     /translation="MRQTGTVKFFNQTKGYGFIRPDSDANKDVFVHITAVERSGIGPL
                     DEGMRVSFEMEPDKRGKGPKAVNLQLVT"
     gene            complement(6932..7537)
                     /locus_tag="PK264_18035"
     CDS             complement(6932..7537)
                     /locus_tag="PK264_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HRD77808.1"
                     /translation="MRLYVTLTSPYARLARIVVREKGLEAEVVEIVAETRKPGSPYYR
                     INPSGRVPYLVRDDGIGMEDSQLICLYLDHLGGAPALHLPFETGNWAYGRIEATARSL
                     CDGISVWVREMRRPAGERSPNILAHEIERARRLADVFEHAVRGPPLAERVTMASLLLL
                     VAVDSSRFSEMDDLEATRPTLAAWAKELRRLPSVAATAPKK"
     gene            complement(7707..>8090)
                     /locus_tag="PK264_18040"
     CDS             complement(7707..>8090)
                     /locus_tag="PK264_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77809.1"
                     /translation="MRVQNPVRFAEVLLGEDKGWSPDRIKSLISGGPMNNQITLTKKI
                     PVHITYFTAHVSDDGKVSYKPDIYGHEERILLGIDGKFHLIPKPAPAPDPVAVARAAG
                     PPVAKSKYGSNNPGFAGFFNGLFGN"
BASE COUNT         1419 a         2668 c         2621 g         1382 t
ORIGIN      
        1 agcgagcggc ccgcgcgcac cttctcgacg gctgcgcgcc agcgcggctc gggccattgc
       61 caggcgggct gctcgggcgc ggctttgggt tcactcgcca ttcaggcgct ccgtgcctat
      121 ggtctgaaag atgttactac ctagtgagac gttagccttg caccgggtcc cgtccaaggt
      181 cctctttgca ggcttccatt gaagtccatg tttgaagcgc ccccggcacg atgctgtgac
      241 gactgtggtc attgctcgga cattcccgcc ggtcactcgt caattggagg ctcgcatgca
      301 cattatcgca gccctcgtcg ccgctgccgg cgtcgtcctc ttcatcctct atcgcatgca
      361 gcaggccgca acggccgccc gcgacgtggc agaagcggca agcgatctct ccggcctctt
      421 ccgcagctgg aactggcgcc ggcgcgcgaa cgtcaacccg ctcgatgcga tcgaggaccc
      481 gcgcgaggca gcggccgccc tgatggtagc gcttgcccag tacgacggcg ccgtgaccga
      541 ggccgagagc cgcgtcatcg tcggtgagat gacgaagcat ttcggagcga cggcgagcga
      601 ggccggcgag ttgctcgcca gggggcgctg gctggcgccg gagcgtgccg acgtcggcga
      661 gttgatgcgg cgcctgaagc gcgtcatcga ggcgaagtgc ggcccgaagg agcgccgcga
      721 gctgatcgcc atgctgcgtg ccgtcgcaaa tgcaagcggc acgcccgacg agacgctcga
      781 gcacgacatc gccaatctcg agaagtcgct cgccgcctaa cccgccccgc ccggatcgac
      841 gcccgcaatc tcggccatct gccgtgcaat catctggtcg aggtggccct cgaagggcac
      901 ggtgaaatag gcaaggccgc gtgcctcgga acgccacgcc gcgaactgtg ccgcaaatgc
      961 cgccggcggc tctcccgacc cttcgagcgc gatgaccgag gcggctagtg ccggctgggt
     1021 catcggcagg aacggtatgg tgatccggct gaccgtgccg tccggcatgc gctgcggcgc
     1081 accgagaacg gcacagcaca cgatcgccgg cggttcggca aaggccagga cggcgccgac
     1141 gaccatccgg ctcgcctcgc ttcccggcgg cgtgcgatag gtccagacct gcccggctgc
     1201 gaacatgcgg accctccatg acaatacatt gcccgtgccg gggctcagtc gctcgcgcgc
     1261 agcaaggcgc cgccatcgac gggcagggcg acgccggtga tgaacttcgc ttcgtccgag
     1321 gcgaggaaca ggcaggcata ggcgacgtcc cacgccgagc ccatcttgcg cttcaatggc
     1381 accttggcat cgcgctcggc ggcgacatcg gcacgagtgc gcccccacac gcgcgcccgc
     1441 gtgtcgacgg ccatgggcgt gtccatcagg cccggcagga tcacgttggc gcggatgccg
     1501 aactcggcgt tctgccgcgc gatctgctcc gtataggcga tcatgccggc tttcgtcgtc
     1561 ttgtaggcga ctgtcttgta ggtggccgag agggctgcaa ccgacgagat cgacaggatc
     1621 acgcccgact gctggcggcg gaacacggga agcgcgtgct tcacggccat cacgaagccg
     1681 cgcaggttga ttgcgttgac gcgatcgaag gcctcctcgg tgatctcggt cggtgaggcg
     1741 tcgccgccgc cgagactgac tcccacattg taatggaggc agtcgatccg cccgaagcgc
     1801 ttcaccgcct cctcgatcgc ggccttcatg tcggcttcgc gcacgacgtc gcccttgaag
     1861 gcaagcgtgc tgcccttggc atccttgttg gcgagcactg cagtctcctc ggcgctcgcg
     1921 aggtcgcgat caacggccag caccttggcc ccttcgcgcg cgaacagcat ggccgtcgcg
     1981 cgtccattcc ccatgccctc gcccgggctc tgacctgcac cgatgatgac ggcgactttg
     2041 tccttgaggc gcatctgtcg ctccctttcg aatgtctgaa tgccgtcggt cctcaggacc
     2101 tgggcagcgg atacctgtcg aggtagggct gatactccgg ctccacgtcg atctcgagcg
     2161 agccgagcag ccgcacgacg gcattgtaga acgagatcgt gatgacgaga tcggtcacgg
     2221 actcgttcga cagcgatttc ctgagctccg caaaggtcgc cttcgagacg gcgccatcgc
     2281 gcgtgacctc gcgcgcggcc cttagcgccg tcttgacgag cggctcgagc gggttcggct
     2341 tcccgtccgt gtcggcgatc agcgccttga tatcgtcgtc cgtcacgccg aagtcgtagc
     2401 cgagcttcac atgatgcgac cattcgtagg gcgagcgggc gagccagccg acctgcagta
     2461 tcgcgagttc ccggagccgc ggatcgagct tggacttgta gcggatgtag tggccgaggc
     2521 cgtggaacgc tcgcgcgcct cccgggctgt tgacgagagc ccggtagagt gtgatcggcc
     2581 gtgccaggag atcgtgatcc gcggctgcga gatcggattt ttcgagatag ggaacgcgag
     2641 ccattggggg tcctcgacgg ttttgaccgc ggggacttta ggccgaggcg ctccgaattg
     2701 catgagtgtt gcgcgcggga ctggtcggca gtgggcgcac gcgaaggtca gcgcgagcag
     2761 acgttcgcac tcgccgtgca gctgtagagc cccgtatccg gcccgaacag gcggtagggc
     2821 acgcattgca cttggcgttc ggcccgcgcg agcatgaccc cggcggcact caactccgat
     2881 ttgacctcgc cgatctgctc ggcgagacgc acctttgcga aataggccgc aacgtcgcga
     2941 ttggccatcg tgactgtcgc agaggcgaca tcgcagactg gaccgcgagg cgccggcacg
     3001 ctctccagca tggcggtcga gcaggccgac aaggagacgc cgaagccgat cgccgcaatg
     3061 gcgatgaccg gggaacgccc gaatgacttc ctcataagac tttgcctcac ccgactgaac
     3121 ccacacactg gaggctaagc cacatgagtt aagaagcgat tgcggctcgt ttcagattga
     3181 aacaaggcaa ggtcttgatg ctttgcgcgg ttgaaaatcc acgccggtct gtggtccgac
     3241 acgtcggccg tgcaaagatc ggccgcggcg acaaagtcag cgtactgccg cgactgccga
     3301 gcaggatcag attgccggac gcttcttgag gccggcctcg gcgtcgacga aggggccctt
     3361 cacctcggtg ccgacgttga cgagcgtcgt gaccagggcc acggcaatga atgcggcgat
     3421 gaggccgtac tcgatggccg tcgctccgct ctcgttccgc cagaaaatag caagaaggcg
     3481 ctgcatgctc gttccccgtc acatctgcgg ctggttgctc gtcgtcttgg cgatcgacca
     3541 tagctgatga tcgttaatgg gccgtcctgg ctcgccgcgc catgtcgtgc attggttgca
     3601 tgatcgttac gcgctacgac cgaattgccg gcaacacctg tgcgcgctaa tcagcgcgcg
     3661 ggaacgcgtg acacctcaat gcctccggtt cgctgacgct cacgttcgag ttcaggagca
     3721 gggacaggat ttcaagggac aacgggcgtg gaaacctacg actacgtgat catcggcgcg
     3781 ggctcggcgg gcagcgttct cgcggcgcgg cttagcgagg atgccggttc gaacgtctgc
     3841 gtcctcgagg ccggacccaa ggactggcac ccctacattc atattccggc gggcttcatc
     3901 aagacgttct acgacaagcg cgtgaactgg ctctactcga tggagccgag cgagtggacc
     3961 ggtggccggc gcattcatgc tccgcgtggc aagacgcttg gcggctcaag ctcgatcaat
     4021 ggccacatct acaatcgcgg ccagcggctc gatttcgaca cctggtcgca gttcggcaat
     4081 cgcggctggg ggtatgccga cgtgctcccc tatttccgcc gcatggagcg ccgcgagggc
     4141 ccgagcgatc ctacctatcg cggcaaggat ggcgccctca cggtcagcaa catcgattgg
     4201 acgcacccgc tctgcgaggc cttcatcgaa ggcgccgtca gtctcggcat tccgcgcaac
     4261 ccggactaca acggcggcat ccaggaaggc gtgtcctacg cccagcgcac gatcgagcgc
     4321 ggctggcgca tgagcgcggc caaggcattc ctgaagcctg ccatgaagcg gccgaacctc
     4381 aaggtcgtca cccatgccca tgcgacgagc atcgtcctcg agggtaagcg tgcagtgggc
     4441 gtgcgctacc tgaagggcgg tcgcaacggg acgcaagtcg agattcgcgc ccggcgcgag
     4501 gtgatcctga gcggcggcgc ctacaattct ccgcaactcc tccagctctc gggcatcggt
     4561 ccggccgagc agctcgcctc gcacggcatc acggtgcatc acgcgctgcc cggcgtcggt
     4621 gagaacctca gggatcacta cgcaccgcgc ttcgtcgccc gcgtgaagaa catcgagacg
     4681 atcaacgagc gcgtgcgcgg cttgaaactc gtcggcgagg ccatgaagtg ggcggccgga
     4741 cggccgagca tcctcggctt gagcccgacg ctcatctact gcttctggcg ttcgcacccc
     4801 gacatcgcca actccgacat ccagctgaca ttcacgccgg caagctatcg cgaaggcgtc
     4861 cagggcgagc tcgagcgcga gcctggcatg acggtcgcct cctggcagca gcggcccgac
     4921 agccgaggct acgtgcggct gcgctcagcc gacccctttg cgccgccgat catccagccg
     4981 aactacctcg ccgaggaggg cgaccggcgc gtgctgctcg cggccatgcg cctcgcccgg
     5041 cggctgctcg cctcgaagcc gctcgagcac tacacagcct atgaggactt cccgggaccg
     5101 aaggtgcagt cggacgacga attgctcggc gccgccaagg agcgtggcac gacgacgttc
     5161 catcctgccg gcacctgccg catgggaccc gggaccgaca agctcgcagt cgtcgacgat
     5221 cagctgcgcg tacgcggcct cgaaggactg cgtgtgatcg atgcctcgat catgccgacc
     5281 atgctgtctg ccaatctcaa cgcggcgacg atgatgatcg ccgagaaagc gtccgacatc
     5341 atccgcggtc gcgccccgcc agagccgctc gtcaacgcat gaactaccgg cacgccttcc
     5401 acgccggcaa ctttgccgac gtcgtcaagc acgtcatcct gtctcgcatc ctgctgcatc
     5461 tcggcaggaa gccggcgccc ttccgcgtcg tcgatacgca tgcgggcgca ggccggtaca
     5521 gtctcgcagg cagcgaggcg gcccgcagcg gggaatggca gggcggcatc ggtcgcctga
     5581 tcggaagcgg cgcggcgcca ctgccctccg acattcagca tctgatgtcg ccgtatctgg
     5641 acatcgtccg gcgcgccaat tccgacggcg gcttctcggt ctatcccggt tcgccgatga
     5701 tcgcggcggc gatgctgcgc ccggacgacc acctggtcgt caacgaactg cagccggacg
     5761 agcacgcggc actcgcttcg gcaatggccg gcgaccggcg cgtgaccacg ctgtcgatcg
     5821 acggctatgc cgcggtgcgc gcgacgctgc cgccgaagga gcggcgcggc ctcgtgctca
     5881 tcgatccgcc gttcgagaag ccgggcgaac tcgaccggct cgtcggcgcc atcaaggacg
     5941 gggtgaagcg gttcgcgacc ggcaccttca tgatctggta tccgatcaag gatctgcgca
     6001 gcgccgagcg gcttcacgtc gcgctcgccg cgactggaat tgccaagctc ctggcgatcg
     6061 acctgacagt ccgtcatccg gtccgtaacg ggcgcgtcct cgccggcgcc gggcaggtga
     6121 tcctcaatcc gccgttcacg ctggccgggg aaatggaggc aattctccca gtcctcgcga
     6181 cacgcttcgc cgttgcaccc ggcgcatcgg catcggtgaa ggcgatcacc agctgaatgt
     6241 gcagcgcagc aaagccgaac tctctacttg cgatcttcag ccggatcgcg cgccaattca
     6301 tggtacgcgc aacaatgggc aattttgact tgcgtcaatc tgtctcgtgg acgataatat
     6361 tcccgttcgg gcggcctaac ggacccggcg gagactgatg agcagagcag aaaaattgcg
     6421 gctgtgttcc acctcgagtc gcacctcacg ttccccgaca tcggacgaga ctgcctaggc
     6481 accacctccc cgtccgaagg ggcggggtcg accaacgctt tcggggaaaa ggagcctcgc
     6541 atgcggcaga ccggcacggt caagttcttc aatcagacca aggggtacgg cttcatccgg
     6601 ccggacagcg acgccaacaa ggatgtcttt gtgcatatca ccgcggtcga acgatcaggc
     6661 atcggcccac tcgatgaggg catgcgggtg tcgttcgaga tggagccgga caagcgcggc
     6721 aagggtccaa aggccgtcaa tttgcagctt gtgacctgag cgtcgaatat aactacccat
     6781 cggacacgtg cgacggcgac ggtcaaatcg caacggtcgt atccatgatc ttgtccagcg
     6841 ggcacggttg ggcccgctga cgcgctccgg gtgtcccaaa ttgataccag ccggagtgga
     6901 cgtcagctcg tgcgcaattt gggcgccaga ctcatttttt cggggctgtc gcggcaacag
     6961 atggcaggcg gcgcaactct ttggcccatg ccgcaagcgt cggacgtgtc gcctcgagat
     7021 cgtccatctc ggagaagcgc gaagagtcga cggccaccag cagcagaagc gatgccattg
     7081 tcacccgctc agccagcggc ggaccccgca ctgcatgctc gaatacgtcc gcaagccgcc
     7141 gcgcccgctc gatctcgtgc gctaggatgt tcggcgagcg ctcgccggcc ggccggcgca
     7201 tctcccgcac ccagaccgag atgccgtcgc acaggctccg cgccgtggcc tcgatccggc
     7261 cgtaagccca attgcctgtc tcgaaaggga gatggagcgc cggggcaccc ccgaggtgat
     7321 cgaggtagag gcagatgagc tgactgtcct ccatgccgat cccgtcgtcg cgcacgagat
     7381 aggggacgcg cccggaagga tttatccggt agtagggact gccaggtttg cgggtctcgg
     7441 ccacgatctc cacgacctcc gcttcgagcc ccttctcgcg gacgacgatg cgggcgaggc
     7501 gcgcgtaggg cgaggtcagc gtcacgtaga ggcgcatcgc tgcagaattc ctctgatcgg
     7561 attgcagatg cgctgatcct gcggagcttc gccgcgatca gccaccgacc aatctgcggc
     7621 cacgatcgtc gccactggtg aaaacggcga gggcggccaa ggcgccgccc ttcgaggtcg
     7681 acgagtactg cacggccgcg ctgccgtcag ttgccaaaca gtccgttgaa gaagccagca
     7741 aagccagggt tgttgctgcc gtacttgctc ttggcgacgg gcggccctgc agcgcgcgcg
     7801 acggcgaccg gatccggcgc cggggccggc ttcgggataa ggtggaactt gccgtcgatg
     7861 ccgagcagga tccgctcctc gtgcccatag atgtcgggct tgtagctgac cttgccgtca
     7921 tcggagacgt gggcggtgaa gtaggtgatg tggaccggga tcttctttgt gagcgtgatc
     7981 tggttgttca tcgggccgcc cgagatcagc gacttgatgc gatccggcga ccagcccttg
     8041 tcctcgccga gcagcacctc ggcgaagcgg accgggttct gcacgcgcat
//
LOCUS       DAORNH010000209         8061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6592_length_8061_cov_2.255309, whole genome shotgun sequence.
ACCESSION   DAORNH010000209 DAORNH010000000
VERSION     DAORNH010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6592_length_8061_cov_2.255309"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    215..272
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<273..710)
                     /locus_tag="PK264_18045"
     CDS             complement(<273..710)
                     /locus_tag="PK264_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77810.1"
                     /translation="MSIIFAFAFENVPAGLETFWKIQAMMGAAFWIGLYWRRATPAGA
                     WAGTLGAFAVVIVTGTDGFNAWAAANLPEYMIWDGKFRISWQMFSYLSTGFGLCIVVS
                     LLTPRLPKAQLDRFYNCLHTPVRGDEPHMREPFSLPTNVQPDVP"
     assembly_gap    715..886
                     /estimated_length=172
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<887..2529)
                     /gene="pelF"
                     /locus_tag="PK264_18050"
     CDS             complement(<887..2529)
                     /gene="pelF"
                     /locus_tag="PK264_18050"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HRD77811.1"
                     /translation="MRRRAESDEANSERATDISILVEGCYPYVPGGVSAWIDWLIRSH
                     PDLSFSVVALWPRPTRQQPRYALPPNVTALHHLYLQDFGGVARQRPLPVRDLNEVASA
                     LDDLMMVGGRDRLEELMRRLDVIKGRFALGDVFNSPAAWEIARRMYDTEMPYGSFLHF
                     FWAWRALAGGLLATCDFKLPRAKVYHCISTGYAGVLASRAAIQTGRPAILTEHGIYTN
                     ERRIELLMADWVADTVDKGHALDDPRFDLRDMWVKAFEAYARTCYEACREIITLYEDN
                     QRAQRALDAPKERLTVIANGIDLKRFEGVRPPPADARPTMALIGRVVSIKDVKTFIAA
                     AHVLRPRLPNLRAVIAGPLDEERAYADECRTIATELGLDGTVEFAGSVNVAEFLSEVH
                     VAVLTSLSESQPLVILEAGAAGIPFVATNVGSCREIIEGRSDENPPLGIGGYITGLVA
                     PGEINGFHIAMFGLNALIGIVTQPHIMGVCAAGRSELDGRVGFACGNLLKRVCTVAWM
                     LTGLCGVALYMKTRPDLHPDLVYGQMARELLPTILPGLVG"
     gene            complement(2526..>3548)
                     /locus_tag="PK264_18055"
     CDS             complement(2526..>3548)
                     /locus_tag="PK264_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77812.1"
                     /translation="GIIALMIAAGEAVALAMALASGASFVHVLAIHAVATVALAIWCT
                     FTWRGGGDVAIPVMAFLISLVAGPLGALGAGALAWIGRPTGADQPRLLAGWYARIANS
                     ITTDPVSQLCEHVAVGRAVDLSEEPPTSFLHTMSSGPLVERQTVLGIVARKFHFDYLP
                     VLRAALDNPEPVIRVQAAAVAARVRGDLKSEMDRLIAAAASPPIDIGGRLALAAKLDL
                     GIEARLVDQITAQAAGAARDTLLDGCDEREVANWCQRARRHRQLPPFDLAAIRAGGQH
                     AELPSPQWIDAPRLVDSFERRLIRAGRYAALRRVRALLRAGAAHPGARLRSIELRDAS
                     PSAAGT"
     assembly_gap    3549..3565
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3606..4535)
                     /locus_tag="PK264_18060"
     CDS             complement(3606..4535)
                     /locus_tag="PK264_18060"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRD77813.1"
                     /translation="MLGTFALGWPEQDIGENHFTYLLRILAQPVLWIIAALLVGQFRL
                     RQVEHQQEMERQIGELTAQRAAIADYSLHLRSRCDMLERHIASQKAKAVPDPLVELER
                     LVASEPNELAECFDRAVRTVFGMASASLLERDGDRLKVIATSGWASDARWRRELAGAS
                     DFYNAVVVEGRALTVLDPEDERELAGEGLLALPIHSPNADTVLGVIKIEQIEPAMLSD
                     ETPARLAVISRLLVPALASRPAARLSPRLASVAQSPLGSAALATSAGSPKRSSPMVSM
                     WRRLRWLPPANDPADEAAAPVEPSAAPGSRGAT"
     gene            complement(4781..4972)
                     /locus_tag="PK264_18065"
     CDS             complement(4781..4972)
                     /locus_tag="PK264_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77814.1"
                     /translation="MLQAFSTTLQASAGSLVVPPESAEMIEVIELLQQLPLDGTVVTG
                     DAAFTFRTIIEAIRERGGD"
     gene            complement(5115..6257)
                     /locus_tag="PK264_18070"
     CDS             complement(5115..6257)
                     /locus_tag="PK264_18070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRD77815.1"
                     /translation="MSTPFSPPLERPSGLEAEFQTQHEFIKAARMRLNPMIWDYLVGG
                     TETETTLKRNRAALDRIALRPRVLVDVSRIDPARALLGKKLRMPILLAPVGSLESFDA
                     GAGVTAAKAAEQYGCGMMLSSVSTLEMEAVTSAAKGGLNIYQLYVRGDDAWIDERVKR
                     AVAAGFNAYCLTVDTAVYSRRERDIAKRFAKPWRTSVPPAGVIAQARLTWKNVAHFKK
                     AHKIPLILKGIATAEDAKLAVEHGVDVIHVSNHGGRQLDHGRGAMDVLPEVLEVARGK
                     AQVIVDGGFSRGTDIVKAIAMGADAVALGRLYCYALAAAGQAGVVRMLEILEAEVISA
                     MGLIGVTKLDELGPRHLHVGAPAVMEPSVFSAFPLLNLDDEGYGGR"
     assembly_gap    7189..7379
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7755..>8061
                     /locus_tag="PK264_18075"
     CDS             7755..>8061
                     /locus_tag="PK264_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77816.1"
                     /translation="MLRAMLVVIAALTFGLEAARAGPNDIAIIIANENYAGTVPKAGF
                     AHRDGDEIKRLAREVLAVPESRIVLLKDATSGTLSDWFEAGGGVGPDLARLPVRADTV
                     "
BASE COUNT         1269 a         2534 c         2611 g         1209 t
ORIGIN      
        1 cggcggcgat tgtcctctcg ccgattgcca ggacaaactt atgcgggcgc tggaggaatt
       61 ccgcagcggc acgcgattcg acgacgacat ctcgctcgtg tcgctggaag tgttgccgta
      121 gcgcgggccc cgactcaggt gccccacttc gccatggcga acacgaacca taccatcagc
      181 cctacgcaaa cccagagtgt gacaaaaccc agcannnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggcacat ccggctgaac attcgtcggg
      301 aggctgaacg gttcccgcat gtggggctca tcgccgcgta cgggcgtgtg gaggcagttg
      361 tagaagcggt cgagctgggc cttcggcagc cgaggcgtca ggaggctcac gacgatgcac
      421 agcccgaacc cggttgagag gtagctgaac atctgccacg agatgcggaa tttcccatcc
      481 cagatcatgt attcgggaag gttggccgcc gcccatgcgt tgaatccatc cgttcccgtg
      541 acgatcacca ccgcgaatgc gccgagggtt cctgcccacg ccccggcggg cgtggcacgg
      601 cgccagtaga ggccgatcca gaaggccgct cccatcatgg cctgaatttt ccagaaggtc
      661 tccaatccgg cgggcacgtt ctcgaaggca aaggcgaaaa tgatactcac gacgnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccga cgaggcccgg
      901 aagtatcgtc gggagcaact cgcgcgccat ctgtccatac acgagatcgg ggtgcaggtc
      961 cgggcgcgtc ttcatgtaga gcgcgacgcc acaaagcccg gtgagcatcc acgccacggt
     1021 acagacacgc ttgagcaggt tgccgcacgc gaatcccacg cgaccgtcga gttccgagcg
     1081 gcccgccgcg cagacgccca tgatgtgggg ctgggtcacg atgccgatca gggcgttcag
     1141 gccaaacatg gcaatgtgaa atccattgat ctcgccgggc gcgacgagcc ccgtgatgta
     1201 gccgccgatg ccgagcggcg ggttctcgtc cgagcggccc tcgatgatct cgcggcagga
     1261 gccgacgttc gtcgcgacga acgggatgcc ggccgcaccg gcctcgagga tcaccagcgg
     1321 ctggctctcg gagagagacg tgagcaccgc cacatgcacc tcggagagga actcggcgac
     1381 gttgaccgag cctgcaaact cgactgttcc atcgaggccg agctccgtcg caatcgtgcg
     1441 gcattcatcg gcataggcgc gctcctcgtc gagcggcccg gcgatcacgg ccctgagatt
     1501 cggcagccgc ggcctgagca catgggcggc cgcgataaac gtcttaacgt ccttgatcga
     1561 gacgacgcgg ccgatgagcg ccatggtcgg ccgggcgtcg gccggcggcg gccgcacacc
     1621 ctcgaagcgc ttgagatcga tgccgttggc gatgactgtg aggcgttcct tcggcgcatc
     1681 gagcgcgcgc tgggcccgct gattgtcctc gtagagcgtg atgatttccc ggcatgcctc
     1741 gtagcaggtg cgggcatagg cctcgaacgc cttcacccac atgtcgcgaa gatcgaagcg
     1801 cggatcgtcg agggcatggc ccttgtcgac agtgtccgcg acccagtcgg ccatcaggag
     1861 ctcgatgcgg cgctcgttcg tgtagatgcc gtgctccgtc aggatcgcgg gccgccccgt
     1921 ctggatcgcg gcgcggctcg cgagcacgcc tgcgtagccc gtcgagatgc aatgatagac
     1981 cttggcccgc ggcagcttga agtcgcacgt ggcgagcagt ccgccagcga gcgcacgcca
     2041 cgcccagaag aagtgcagga acgacccgta tggcatctcg gtgtcgtaca tgcgccgggc
     2101 gatctcccag gcggcgggcg agttgaaaac gtcgccgagg gcaaagcgcc ccttgatcac
     2161 gtcgagccga cgcatcagct cctcgagacg atccctgcca ccgaccatca tcaggtcgtc
     2221 gagggcggag gcgacctcgt tgagatcgcg cacgggcagc ggccgctgcc gggcgacccc
     2281 gccgaagtcc tggaggtaga ggtggtgcag ggcggtcacg ttggggggta gcgcgtagcg
     2341 cggctgctgg cgcgtcgggc gcggccagag ggcgacgacg gagaagctca gatcgggatg
     2401 cgaccggatg agccagtcga tccaggccga tacgccacct ggcacatagg ggtagcagcc
     2461 ttcgacgagg atcgagatgt cggttgcccg ctcgctgttc gcctcgtcgc tctcggcacg
     2521 gcgcctcatg tgccggccgc cgacggggac gcatcgcgca gctcgatcga tcggagccgg
     2581 gcacccggat gcgccgcgcc agccctcagc aacgcgcgaa cccggcggag cgcagcgtag
     2641 cgacctgccc ggatgaggcg gcgctcaaag ctatcgacga gccgcggggc atcgatccac
     2701 tggggtgatg gcagctccgc atgttgcccg cctgcccgga tggccgcgag gtcgaaggga
     2761 ggcagctgcc ggtgccggcg cgccctctgg caccagttgg cgacctcccg ctcgtcacag
     2821 ccatcgagca gcgtgtcacg cgccgcgccc gctgcctgcg ccgtgatctg gtcgacaagg
     2881 cgcgcctcga tgccgaggtc gagcttggcg gcgagcgcca gtctgccacc gatgtcgatt
     2941 ggcgggctgg ccgcggccgc aatcagcctg tccatctcgg acttgaggtc gccgcggacg
     3001 cgggcggcga cggcggcagc ctgcacccgg atgaccggct cggggttgtc gagggccgcg
     3061 cgcagcacgg gcaggtagtc gaagtggaac ttgcgagcga cgatcccgag caccgtctgc
     3121 ctttcgacaa gaggtccgga ggacatcgta tgcaaaaacg aggtgggtgg ctcctctgac
     3181 aggtcgaccg cccgaccgac ggccacatgc tcgcacagct gtgaaacagg atcggtggtt
     3241 atcgaattgg cgatccgcgc ataccagccg gcgaggagcc gcggttgatc ggcgccggtc
     3301 ggtcgcccga tccaggccag cgccccggcg ccaagcgctc cgagcggacc cgcaacgagc
     3361 gaaatgagaa atgccatcac cggaatggcg acgtcgcctc cgccgcgcca ggtgaacgtg
     3421 caccagatcg ccagcgccac cgtcgccact gcgtggatcg cgaggacatg aacgaacgac
     3481 gctccgctcg ccagggccat ggccagggcg acggcctctc ccgcggcaat catcaacgcg
     3541 atgatcccnn nnnnnnnnnn nnnnngcgac gcgtgtcgca gccggcgcct cagctccgac
     3601 cgcgctcatg tcgcgcctcg cgatcctgga gcggcgctcg gctcgacggg ggccgcggcc
     3661 tcgtcggcgg gatcgttggc cggcggcagc catcgcagcc gccgccacat cgacaccatt
     3721 ggcgaggaac gcttcggcga gccagcagag gtcgcaagcg cggccgagcc gagcggcgat
     3781 tgtgcgacgc ttgctaggcg cggcgacagc cgggcggccg gccggctcgc caacgctggc
     3841 accagaagcc gcgagatcac cgcgaggcga gcgggcgtct cgtcactgag catcgccggc
     3901 tcgatctgct cgatcttgat cacgccgaga acggtatcgg cgttcggaga atggatcggc
     3961 agcgcgagga ggccctcgcc cgcaagctct cgctcgtcct ccgggtcgag cacggtcagc
     4021 gcccgtccct cgactacgac cgcgttgtag aaatcgctgg cgcctgcgag ctcgcgtcgc
     4081 cagcgggcgt cgctcgccca tcccgatgtg gcgatcacct tgagcctgtc gccgtcacgc
     4141 tcgaggaggg acgcggatgc catgccgaat accgtgcgca cggctcgatc gaagcattcg
     4201 gcgagttcgt tgggctccga ggcgaccagc cgctcgagct cgaccagggg gtcgggcaca
     4261 gccttcgcct tctggctcgc gatatgacgc tcgagcatgt cgcagcgcga ccgcagatgc
     4321 agcgagtagt cggcgattgc cgcgcgctga gcggtgagct cgccgatctg tcgctccatc
     4381 tcctgctgat gctcgacctg ccgcaggcgg aactggccga ccaacagtgc cgcgataatc
     4441 cataggaccg gctgtgcgag gatacggagc agataggtga agtggttttc gccgatgtcc
     4501 tgctccggcc aaccgagcgc gaaggtcccg agcatacgaa tcgtggtctt ctcaagatcg
     4561 ctatcgctgc aagcaccaac cttgtgcagc tcgcgcgcgc tatccatgat ctccaaaggt
     4621 atggcccatg tcgatcgtct cgtcgcgttt caggtcgggc gggtcacgcc acttggtctg
     4681 cctcggctcc gcggcgatca cgtcttgagg gggaaacgtc gccgaaggcg cgagcggtct
     4741 cgccttcgag ctctggctgg ttggctttga cgaacaggaa ttagtcgccg cctcgttctc
     4801 ggatcgcctc gatgatcgtg cggaacgtga aggcggcatc accggtgacg acggtaccgt
     4861 cgagcggcaa ctgctgcaaa agctcgatga cctcgatcat ttcggcgctt tcgggcggca
     4921 ccacgagcga gccggcggag gcctggagcg tcgtcgagaa cgcctgcagc atgtgcacgc
     4981 ccgggctggt ctcgtgctgg ccgccgcgct gccgcttgcc gtcaacggcg acatgaccga
     5041 ggccatgatc gcaaagtgcg ttcagcgtac ggcaaaatgc cctgctcgcg gagccgccat
     5101 ttgcggttgc cgcctcaccg gccgccgtag ccttcgtcgt cgaggttcag gagggggaag
     5161 gcgctgaaca cgctgggctc catgaccgcg ggggcgccca cgtgcaggtg ccgggggccg
     5221 agttcgtcga gcttcgtcac gccgatgagc cccatggcgc tgatcacctc agcttcgaga
     5281 atttcgagca tgcgcacgac accggcctgg ccggcggcgg cgagcgcata gcaatagagc
     5341 cggccgagcg cgacggcatc ggcccccatg gcgatggcct tgacgatatc ggtgccgcgc
     5401 gagaagccgc cgtcgacgat cacctgagcc ttccctcgcg cgacctcgag gacctcgggc
     5461 agtacgtcca tggcgccgcg gccgtgatcg agctggcggc cgccgtgatt ggagacgtgg
     5521 atgacatcga cgccgtgttc gaccgcgagc ttggcatcct cggccgtcgc aatgcccttc
     5581 aggatcagcg ggatcttgtg cgccttcttg aagtgggcga cgttcttcca ggtgagcctc
     5641 gcctgggcga tgacgcctgc tggcggcacg ctcgtccgcc acggcttggc gaagcgcttg
     5701 gcgatgtcgc gctcgcggcg gctgtagacg gcagtgtcga cggtgaggca gtaggcattg
     5761 aagccggcgg cgacggcgcg cttgacgcgc tcatcgatcc acgcgtcgtc gccgcggacg
     5821 tagagctggt agatgttgag gccgcccttg gccgccgacg taacggcctc catctcgagc
     5881 gtcgagaccg aggagagcat catgccacag ccgtactgct cggcggcctt tgccgcggtg
     5941 acgccggcgc cggcgtcgaa gctctcgagc gagccgacgg gggcgagcag gatcggcatc
     6001 ctgagcttct tcccgagaag tgcccgcgcc ggatcgatgc gcgagacatc gacgagcacg
     6061 cgcgggcgga gcgcgatgcg atcaagggct gcgcggttgc gcttcaaggt cgtctcggtc
     6121 tcggtgccgc cgacgagata gtcccagatc atcgggttga gccgcatgcg ggcggccttg
     6181 atgaactcgt gctgggtttg gaactcggcc tcgaggccgc tcggcctctc gaggggcggg
     6241 gagaagggtg tcgacatgag ggctccgggc gaaagcgggt cgcgcactgc tcgtgttgcg
     6301 gcgctgacat ttgcttcccg ttcaggcttg gtcgggaaca aatgtcagcg ccaccaggaa
     6361 cacgggcaac actatgtacc tgacgtttgg ctgcgagagc agtcgtgaaa ggggaccaaa
     6421 cgtcagctgc gctgcactag catctcgaaa caacgcgcgg aacgccgaac cttgcaacgc
     6481 cgccgggccg aaacggctcg cgcgcgatcg ccttccgacc cggcggcgcc tgcgaaggcc
     6541 gatggcacgc cggttccgcc tggtcgcgct gattgcgctg aagtggcccg ccggacgctg
     6601 ctcgctgccg ggaatgatcc tggacgagcg ctggtgcact ttccggggaa ccggcgctga
     6661 gttggccctg ggcgcaactt cgcccaccgc cgccgcgcgc gggacggctg gacactgatc
     6721 gtgttggatc ggccgcaact gctcacctgc acgatgacct gggaccacga cttcgcgagg
     6781 aggacgaggg cggggcgagt atcgcgcgcg acgcctcgca gggacatggg ttggcgcaga
     6841 gcggagtgaa gcccatcggc ctcgggagag gccgtgagcg ccagacctcg cgaaggctca
     6901 gcccagccga cgtcctgatc gagccggctc gctgcccggc cgggtctcgc tctgagttcg
     6961 cgcgccgacc gtgcccgggc ctaggcctgg gccctttttg cgcgtccaca ccccagacgg
     7021 cgccatgccg catattggct cgggggcagg ctgcagtggc gcctggatgt tggaaagatc
     7081 tggtaggcaa gttcgccacc agcctcacaa cgccctactt ccggctcccg catccgcgga
     7141 ggccgcctga cgacaagacg agcggagcgg cggtgccggc tcggatgcnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     7381 gatcacgccc agcacgcgtt accgtcgtcg atcgaccgtt gggtgcatcg acgataatcg
     7441 cagccgtcgc gcgcggggac aactctccgc gatcggtgcg aacgacgcgc atttcgctac
     7501 gcgactgggc ctcgctctgc gtttgcgcgc catccgcgcg catggcggac ccgctcgggc
     7561 ctctgcccgg cccgagcgcg gtccctcatt cgagcccccc acaatcccgt cagggccggc
     7621 ccttgtcagg cacgggctga actcaggcag ccccatgcgg ctccgctcga cctggtgcag
     7681 ggcctgccgc ccgccccggc tcgcgtaccg cgccggcatc tgctagcgtc atcctgattt
     7741 ccaggaggcc ctcgatgctc cgcgccatgc ttgtcgttat cgccgcactg acgttcggtc
     7801 tcgaggctgc acgggcgggc cccaatgaca tcgccatcat aatcgccaac gagaactacg
     7861 cgggtacggt tccgaaggcg ggctttgcgc accgcgatgg cgatgagatc aagcgcctcg
     7921 cgcgcgaggt gctcgccgtg cccgagagtc gcatcgtgct gctgaaggac gcgacgtccg
     7981 gcacgctctc cgactggttc gaggcgggcg gcggcgtcgg tccggacctc gcgcggctcc
     8041 ccgtgcgcgc cgatacggtg a
//
LOCUS       DAORNH010000210         8059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6596_length_8059_cov_2.085707, whole genome shotgun sequence.
ACCESSION   DAORNH010000210 DAORNH010000000
VERSION     DAORNH010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6596_length_8059_cov_2.085707"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK264_18080"
     CDS             <1..598
                     /locus_tag="PK264_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRD77817.1"
                     /translation="GYQPTLATDMGALQERITTTQKGSITSVQAIYVPADDLTDPAPA
                     TSFAHLDATTVLSRSIAEKGIYPAVDPLDSTSRILEPRVIGQDHYDTARRVQAILQKY
                     KSLQDIIAILGMDELSEEDKTTVARARKIERFLSQPFHVAEVFTGAPGKLVDIKDTVK
                     GFKGLCNGDYDHLPEQAFYMVGTIEEAIAKAEKLAEAA"
     gene            679..1005
                     /locus_tag="PK264_18085"
     CDS             679..1005
                     /locus_tag="PK264_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77818.1"
                     /translation="MANPPASGSAANGSITPADGEQGRPPLGTCCSTMADAIGSPDFE
                     PLISIGDDDGVLYISVGLVNTEDTKPGLVEFPLLFCPFCGTAVQTDSEIEQRTGHAPG
                     TSTAGT"
     gene            1062..1451
                     /gene="atpC"
                     /locus_tag="PK264_18090"
     CDS             1062..1451
                     /gene="atpC"
                     /locus_tag="PK264_18090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRD77819.1"
                     /translation="MAATFKFEVVSPERVLLATDAEQVLVPGDDGDFTVFSGHAPVIS
                     TLRPGILEVKGPGGARRLYVKGGFAEVEPDRLTILAASAIDIADGARERIRSELAAAE
                     SELASASGDDAKRLAIEAVESLRRISA"
     gene            1484..>1789
                     /locus_tag="PK264_18095"
     CDS             1484..>1789
                     /locus_tag="PK264_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HRD77820.1"
                     /translation="MAKPERPDAARDRRSRSSFGTSRGGWSPAGRPRRLESDDAITLF
                     GLHAVEAALNNPARAIKRLYLTENAERRIEAAVKARGTTIERVAPRDLDRRLGPDTVH
                     "
     assembly_gap    1790..1799
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1800..>2012
                     /locus_tag="PK264_18100"
     CDS             <1800..>2012
                     /locus_tag="PK264_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRD77821.1"
                     /translation="GALAEVEALPEPELEALAVAGPAAGPVLVLDQVTDPHNVGAILR
                     SAAVFGAAGLVMTRRHSPPLTGALAKA"
     assembly_gap    2013..2114
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2115..2324
                     /locus_tag="PK264_18105"
     CDS             <2115..2324
                     /locus_tag="PK264_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRD77822.1"
                     /translation="AEARIETEPLTGPVALVLGAEGKGLRQLTRETCTRLCRITAAGP
                     LDSLNVSNAAAISLHLAAMRRRGDA"
     gene            2511..2586
                     /locus_tag="PK264_18110"
     tRNA            2511..2586
                     /locus_tag="PK264_18110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2544..2546,aa:Thr,seq:cgt)
     gene            complement(2591..3154)
                     /locus_tag="PK264_18115"
     CDS             complement(2591..3154)
                     /locus_tag="PK264_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77823.1"
                     /translation="MRCLAVAIGADNGVPSASPNGLINLSKLAVWWLRLGIAIERIKP
                     VHPQQNCRHECMHRTLNEEAARPPAANALALQRPADVYTPSPRRYRGLSDIEYPLHGR
                     DGLVTACGRICLHWKNDNQSTVLSGQRVALKKVGDAIGLVSFMTYDLGYIDLEQWTLH
                     PATGHSACGCYPCLRYKPSLMSPGRSI"
     gene            complement(3286..>4082)
                     /locus_tag="PK264_18120"
     CDS             complement(3286..>4082)
                     /locus_tag="PK264_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD77824.1"
                     /translation="LALFERIGRRLVLTATGAGLLDHARTMASAADALALAAEGRAQD
                     ISGRVSISATDAFAAYVLPEIIEHIRATLPQVTIAIVATDSISDLRRREADIAIRHVR
                     PTEPELIARRVMEMQARLYATKAWIDRNGRPSSPADLREAELLGFEPIEQFVGHLAKA
                     GLDVRVQQVRITSENAVVLWEMLRRGLGVGVVLSEVADRMAGIVRLLPEMPAIPVPVW
                     LVAHRELHSSRRIRLVYDAIDAGLQRFAAAGADKPRARKARVRESR"
     assembly_gap    4083..4128
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4299..4943
                     /locus_tag="PK264_18125"
     CDS             4299..4943
                     /locus_tag="PK264_18125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77825.1"
                     /translation="MHITTADERFWNRTARKYAADPIKDMAGYERTLCRTRELLGPTS
                     TVLEIGCGTGSTALLLASSVGQIEASDISSEMIAIAGEKARAAGCCNVSFSVASADGA
                     TVLEGAYDAILAFNILHLVADRRAVLASVRRQLKPGGLLISKTPCLSEMNSLIRLAVP
                     VARLFGKAPSVAFFPGEQLEAEIAASGFTIIERARHGSGRRDPRLFVVARKVET"
     gene            5031..>5241
                     /locus_tag="PK264_18130"
     CDS             5031..>5241
                     /locus_tag="PK264_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77826.1"
                     /translation="MTESALFERLWNGTAEMDEWTRAVSAGAITTITDRVIAIHSSYY
                     CGAVTVIRTREGLVLIDTAGTAFAER"
     assembly_gap    5242..5304
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5305..6308
                     /locus_tag="PK264_18135"
     CDS             <5305..6308
                     /locus_tag="PK264_18135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRD77827.1"
                     /translation="THGHVDHTSGITGIDAEADAAGVPRPRIIAHRNVARRMARYIAT
                     HGYNSIAQAQQFNTQGYVYPIGQRMPDEVYDDTLSLAIGGENLELAHARGETDDATFV
                     WLPRQRTLVSGDFVSWVFPNAGNPRKVQRYAGEWAAALRRMEALQPEVLIPGHGPVVF
                     GRDRAGQMLRDGAEALEYLTSEVLARINKGARLDDVLHAVRVPTSYVSKPYLRATYDD
                     PQFLVRAIYHLNAGWFDGNPANLKPARAAELATEIAALAGGADRLADRARQLASEGRL
                     NLALHLAELAGEAEPGNARVHAERAAVLRALIDGETSLMAKAFYGVFERDAASRSKG"
     gene            6411..7217
                     /locus_tag="PK264_18140"
     CDS             6411..7217
                     /locus_tag="PK264_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD77828.1"
                     /translation="MADRYAHYTSLKFARPKPRVLEVIMSRPGKLNALDATGHREVAE
                     VWRDIDRDPDVSAVILRGEGGVFSAGGDLDMVERMSRDWHERTRVWKEAADLVYNVLN
                     CTKPTVAAIEGPCVGAGLSAAMLCDITIAGKSARIIDGHTRLGVAAGDHSAIVWPLLC
                     GMAKAKYYLMTCETLSGEEAERIGLVSRCVDDDAVIDTAEVAERLAQGAPAAIRMTKY
                     ALNNWFRMAGPTFDASLAMEMMGFSGPEVREGVASLKEKRKPSFKQDNPF"
     gene            7348..>7546
                     /locus_tag="PK264_18145"
     CDS             7348..>7546
                     /locus_tag="PK264_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77829.1"
                     /translation="MTAAENGAAFPVSSGAPLLSVRGVTVRFGGIVALDGVSFDVHAG
                     QIAGLIGPNGAGKTTLFKAILG"
     assembly_gap    7547..7556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7557..>8059
                     /locus_tag="PK264_18150"
     CDS             <7557..>8059
                     /locus_tag="PK264_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD77830.1"
                     /translation="LFNCLSRLYVPSEGEILFEGRSILSAPRHAIAALGIGRTFQNVA
                     LFDKLTVRDNILIGCHSQSTAGFIASALKGPATRAEERRLRETADRLITFMDIEAFAD
                     RRAGGLPFPIRKRVELARALGAKPKLLLLDDIFRERRTVGLIAIRAHALDELQARWLR
                     AGENDQG"
BASE COUNT         1322 a         2609 c         2663 g         1234 t
ORIGIN      
        1 cggctaccag ccgacgctcg cgaccgacat gggtgcgctg caggagcgca tcaccacgac
       61 gcagaagggc tcgatcactt cggtgcaggc gatctacgtg cccgccgacg acctgaccga
      121 cccggcgccg gcgacgtcgt tcgcccacct tgacgccacg accgtgctct cgcgctcgat
      181 cgccgagaag ggcatctatc cggccgtcga cccgctcgac tcgacatcgc gcatcctcga
      241 gccgcgcgtc atcggccagg accactacga taccgcccgc cgcgtccagg cgatcctgca
      301 gaagtacaag tcgctgcagg acatcatcgc catcctcggc atggacgagc tgtcggagga
      361 ggacaagacg acggtcgccc gcgcccgcaa gatcgagcgt ttcctctcgc agcccttcca
      421 cgtcgccgag gttttcacgg gcgcgcccgg caagctcgtc gatattaagg acaccgtgaa
      481 aggcttcaag ggcctctgca acggcgacta cgatcatctt cccgagcagg ccttctacat
      541 ggtcggcacc atcgaggagg cgatcgccaa ggccgagaag ctcgccgaag cggcctgatc
      601 ggtctcccac gcgcgccggc ttcggccgac gcgtacggcg cgctcgaccg gtgagcaagt
      661 ggtctcgacg gagacggcat ggccaaccca ccagcgagcg gtagcgcggc gaacggcagc
      721 atcacgccgg ccgatggaga gcagggccgc ccgccactcg gcacgtgctg ctcgaccatg
      781 gccgacgcca tcggctcgcc cgacttcgag ccgctgatct cgatcggtga cgacgacggc
      841 gtgctctaca tctcggtagg gctcgtcaat accgaggata ccaagcctgg cctcgtcgag
      901 tttccgctgc tgttctgtcc gttctgcggg actgcggtgc agacagacag cgagatcgag
      961 cagcggaccg ggcacgcgcc cgggacgtcc acggccggaa cttgaagcca tgccggtcta
     1021 agggctggca gcgtgagcag cagagattgg agaggcccga catggccgcg acgttcaaat
     1081 tcgaggttgt ctcgccggag cgcgtgctgc tcgcgaccga tgccgagcag gttctggtgc
     1141 ccggcgatga cggcgatttc accgtgttct cgggtcacgc gcccgtgatc tccacgctgc
     1201 gccccggcat tctcgaggtg aagggcccgg gcggcgccag gcggctctac gtgaagggcg
     1261 gctttgccga ggtcgagccc gaccgcctca ccatcctcgc ggcgtccgcg atcgacatcg
     1321 cggatggcgc ccgcgagcga atccggtctg agcttgcggc ggccgaatcc gagctcgcct
     1381 cggccagcgg cgatgacgcc aagcgcctcg cgatcgaagc tgtcgagagc ctgcgccgca
     1441 tttcggcctg atctgacgac gtcgcgggcg ggacaggtgc aacatggcca agccggaacg
     1501 ccctgacgcc gcacgggatc gccgttcgcg ctccagcttt gggaccagcc gcggcggatg
     1561 gagccccgcg gggcggccac ggcgcctcga gagcgatgat gcgatcacgc tcttcggcct
     1621 gcacgccgtg gaagctgcac tcaacaatcc ggcccgcgcg atcaagcgcc tctacctcac
     1681 ggagaacgcc gagcggcgca tcgaggccgc cgtcaaggcg aggggcacga cgatcgagcg
     1741 cgtcgccccg cgtgatctcg accgtcggct gggccccgac accgtgcacn nnnnnnnnng
     1801 gcgcgctcgc cgaggtcgag gcgctgcccg agcccgagct cgaagccctc gccgtcgcgg
     1861 ggcctgcagc tggcccggtc cttgtgctcg atcaggtgac cgacccgcac aatgtcggcg
     1921 cgatcctgcg ctcggcagcc gtcttcggcg cggcaggcct cgtcatgacg cggcggcaca
     1981 gtccgccgct gacgggcgcg cttgccaagg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnngccgag gcgcggatcg agaccgagcc gctcacgggg ccagtcgcgc
     2161 tcgtgctcgg cgcggaagga aaaggcctcc gccagctgac gcgcgaaacc tgcacgcgcc
     2221 tctgtcgcat caccgccgcc gggccgctcg acagcctcaa cgtgtccaac gccgccgcga
     2281 tctcgctgca cctcgccgcc atgcgccggc gcggcgacgc ttgaggcaag aagcggcgcg
     2341 agccgcgccg acgtgcggcg acgccgccac gatcttgccg aagggcgagt gcaatggcgc
     2401 cctcgacctg gcgtctcgag tgagctgacc tgcatgatgt gggcggcagt cgcccggaac
     2461 tctgacttgc aatcacaggg cccgcgcgat atgcgttcgt ctcgtccggg gccgcaatag
     2521 ctcagccggt agagcaccgc attcgtaatg cgggggtcgc aggttcgagt cctgcttgcg
     2581 gcaccatccc ctaaattgaa cgacccggcg acatgagtga cggtttgtac ctaagacatg
     2641 ggtaacaacc gcaagccgaa tggcctgtcg cgggatgcag ggtccactgt tcgagatcga
     2701 tgtatccgag atcgtaggtc atgaaggaga cgagcccaat ggcgtcgccg accttcttca
     2761 gtgcgactcg ctgtcccgag agaacggtcg actggttgtc gttcttccag tgcaggcaga
     2821 ttcgtccaca ggctgtgacg agaccgtcgc ggccgtggag agggtattcg atgtcggaca
     2881 gacctcgata gcgtctgggt gatggggtgt agacgtcggc gggtcgctgc aaagcgaggg
     2941 cgttggcggc gggcggccgc gcggcttcct cattgagcgt gcggtgcatg cactcgtggc
     3001 ggcagttctg ctgggggtga accggcttga tgcgctcgat tgcgatgccg agccggagcc
     3061 accagacggc gagcttcgag aggttgatga ggccattggg cgaagcggaa ggcacaccgt
     3121 tgtcggcgcc gatggcgacc gccaggcagc gcacggcgaa gaagcactcg aaggctgcaa
     3181 cagggacgat tggcgcgaga ctggcttgga cgtcgtcgcg atgcagcggg gttggtcgct
     3241 cgggctgcca ctctgtcgcg ctttccttca gctcggggcg gcaaattagc ggctctcccg
     3301 cacacgagcc ttccgcgccc gcggcttgtc ggcgccagct gccgcgaacc gctgcaaccc
     3361 cgcgtcgatg gcatcgtaca cgagacgaat gcgccggctc gaatgaagct cgcgatgcgc
     3421 gacgagccag acggggacgg ggatcgccgg catttcaggc agcaaccgca cgatgcccgc
     3481 catgcggtcg gcgacctcgc tcagcacgac gccgacgccg agaccgcgcc gcagcatctc
     3541 ccagaggacg acggcgttct ccgaggtgat gcggacctgc tgcacccgga cgtcgagacc
     3601 agccttggcg agatgcccga cgaactgctc gatcggctcg aagccgagaa gttccgcctc
     3661 ccgcagatcg gccggcgagc ttggccggcc gttccgatca atccaggcct tcgtcgcgta
     3721 gaggcgcgcc tgcatctcca tcacgcggcg cgcaatcagc tccggctccg tcgggcgcac
     3781 atggcggatc gcgatgtcgg cttcgcgccg ccggaggtcg ctgatcgaat ccgtggccac
     3841 gatggcaatc gtgacctgag gaagggtcgc gcggatgtgc tcgatgatct caggcagcac
     3901 gtaggcggca aacgcgtccg tcgccgatat ggagacgcgc ccgctgatgt cctgcgcccg
     3961 cccttcagca gcgagcgcca aggcgtccgc ggccgaggcc atggtccggg catgatcgag
     4021 gaggccggcg ccggtcgcgg tcagtacgag tcgcctgccg atcctttcga acagggcgag
     4081 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ccgagccggc
     4141 gggcggctgc cgacagcgag ccggcatcgg ccgtcgccga gaacgcccgc atctggttcc
     4201 aatcgagctg tttcatgcga aagcgtataa tgcacattca gtgctacgca attcgcaaag
     4261 tattcgtatg tgctagtcgg gcggcgaagg agagcacgat gcacatcaca accgccgacg
     4321 aacgcttctg gaatcggacc gcgcgcaagt acgcggccga tccgatcaag gacatggcgg
     4381 gctacgagcg gaccctctgt cgcacgcgtg agctcctcgg cccgacgtcg accgtcctcg
     4441 aaatcggctg cggcaccggc agcacggcgc tgctgctcgc gtcctccgtc ggacagatcg
     4501 aggcgagcga catttcgagc gagatgatcg ccattgccgg cgagaaggcg cgcgctgcgg
     4561 gctgctgcaa tgtcagcttc tcggtggcgt cggcggacgg cgcgacggtc ctggagggcg
     4621 cctacgatgc aatactcgcg ttcaacatcc tgcaccttgt cgccgatcgt cgtgccgtgc
     4681 tggcgagcgt ccggcgccaa ctgaagccgg gtggcctcct aatctcgaag acgccgtgcc
     4741 tcagcgagat gaactccctc attcgcctgg ccgttccggt tgcgcggctc ttcgggaagg
     4801 cgccgtctgt cgcatttttc ccgggcgaac aactcgaggc ggagatcgcg gcgtcggggt
     4861 ttacgattat cgagcgggcg cgccacggct ctggacgcag ggatccgcgc ctcttcgttg
     4921 ttgcgcgcaa ggttgagacg tgaggcgccg gcatggcttg cacatgatgc gattgggcgg
     4981 cggagcgcta aggtgggtgc cgacgctact atcctgtgga ggcccttgcc atgaccgaga
     5041 gcgcgctctt cgagcggctg tggaacggca cagcggagat ggacgagtgg acgcgcgccg
     5101 tctcggcagg cgccatcacc acgatcaccg atcgggtcat cgcgatccac tcgagctatt
     5161 actgcggcgc ggttactgtg atccgcacgc gcgaggggct cgtcctgatc gatacggcgg
     5221 gaacggcctt tgcggagcgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnacacgc acggccacgt cgatcacacg agcggcatca
     5341 ccggcatcga cgcggaggcc gatgcggcgg gcgtgccgcg gccgcgcatc atagcgcacc
     5401 gcaatgtcgc taggcgcatg gcgcgctaca tcgcgacgca cggctacaac agcatcgccc
     5461 aggcgcagca gttcaacaca cagggctacg tctacccgat cggccagcgc atgccggacg
     5521 aggtctacga cgacacactg tcgctcgcga tcggcgggga gaacctcgaa ctcgcccacg
     5581 cgcgcggcga gacagacgac gcgacgttcg tctggctgcc gcgacagcgc accctggtga
     5641 gcggcgactt cgtcagctgg gtatttccga atgccggcaa tccgaggaag gtccagcgct
     5701 acgcgggcga gtgggcagcg gccctccggc ggatggaggc gctgcaaccc gaggtgctga
     5761 tcccgggcca tggccctgtc gtgttcggaa gggatcgtgc tggtcagatg ctgcgggatg
     5821 gtgccgaagc gctcgagtat ttgacgtcgg aggtgctcgc ccggatcaac aagggtgcgc
     5881 gcctcgacga cgttcttcac gcggtgcgcg ttcccacaag ttacgtttcc aagccgtacc
     5941 tacgtgcgac atacgacgat ccgcagtttc tcgtccgggc gatctatcat ctgaatgccg
     6001 gctggtttga cggcaatccg gcgaacctca agccggcgcg cgccgccgaa ctcgcgaccg
     6061 agatcgccgc actcgcgggc ggagcggacc ggctcgctga tcgcgcacgg caacttgcat
     6121 ccgaggggcg gctcaacctc gcgctgcatc tcgccgaact tgccggcgag gcggagcccg
     6181 gcaacgctcg ggtccacgcg gagcgtgcgg ctgtcctgcg cgcgctgatc gacggcgaga
     6241 cgtcgctgat ggcgaaggcg ttttatggcg tcttcgagcg cgatgcggcg agccggtcga
     6301 agggctgacg ggggcgtcgg acgctgccgt ccgccatcgt cagggcttcc gaccggccga
     6361 ggattgaggc tatcctctcg ccaccacaac atcgagatcg gggacacgcc atggcggacc
     6421 gctacgccca ctacacgtcg ctcaagttcg cacgcccgaa gccgcgcgtc ctcgaggtga
     6481 tcatgtcgcg gccgggcaag ctcaatgcac tcgatgcgac aggccatcgt gaggtcgccg
     6541 aagtgtggcg cgacattgac cgcgacccgg acgtctcggc cgtgattcta cggggggagg
     6601 ggggcgtgtt ctcggcgggc ggcgacctcg acatggtcga gcgcatgagc cgcgactggc
     6661 acgagcgtac gcgcgtgtgg aaggaggcgg ccgaccttgt ctacaacgtg ctcaactgca
     6721 cgaagccgac cgtcgccgcg attgagggac cgtgcgtcgg cgcggggctc tccgcagcga
     6781 tgctctgcga catcaccatt gccggaaagt cggcacgcat catcgacggc cacacgcgcc
     6841 tcggcgttgc ggcgggcgat cattccgcga tcgtctggcc gctgctctgt ggcatggcga
     6901 aggccaagta ctacctgatg acgtgtgaga ccctgagcgg cgaggaggcc gagcgcatcg
     6961 ggctggtgag ccgctgcgtc gatgacgacg ccgtgatcga caccgcggag gtcgccgaac
     7021 ggctcgctca gggcgcaccg gccgcgatcc gcatgaccaa atacgcgctc aacaactggt
     7081 tccgcatggc gggcccgaca ttcgatgcct cgctcgccat ggagatgatg ggattctccg
     7141 gccccgaggt gcgggaaggc gtcgcgtcgc tcaaggagaa gcgcaagccg agcttcaagc
     7201 aggataaccc gttctgaacg agttggctgg accaggggcc gcggcctggt gcaaactccg
     7261 ccgcggggcc aagtgcgctt gccgatcggc aacgcgtact tcggcggacg gcgtccggct
     7321 ggcaaggcat tgtcgcgagg gggactgatg acagcagcgg agaacggggc cgcgtttccg
     7381 gtctcctcgg gcgcgcccct cctcagcgtg cgcggcgtga cggtgcgctt cggcggcatc
     7441 gtcgccctcg acggcgtctc cttcgatgtg cacgcgggcc agatcgccgg tctcatcggc
     7501 ccgaacggtg ccggcaagac cacgctgttc aaggcgatcc tcggtcnnnn nnnnnncgct
     7561 gttcaactgc ctctcgcgcc tctacgtgcc gagcgagggc gagatcctgt tcgaaggccg
     7621 ctcgatcctc tccgcgccgc gccacgcgat cgcagcgctc ggcatcggcc gcaccttcca
     7681 gaacgtcgcc ctcttcgaca aactcacggt gcgcgacaac attctcatcg gctgccacag
     7741 ccagagcacc gcgggcttca tcgcgagcgc gctcaagggc ccggcgacgc gcgccgagga
     7801 gcgccggctc cgcgagactg ccgaccgcct gatcaccttc atggatatcg aggcttttgc
     7861 cgaccgccgc gccggcggtc tgccgttccc gatccgtaaa cgcgtggagc tcgcacgcgc
     7921 gctcggtgca aagccgaagc ttctgctgct cgacgatata ttccgcgaac ggcgaacggt
     7981 cggtctgatc gctatacggg cgcacgcgct tgatgaactt caggcgcgat ggcttcgcgc
     8041 cggggaaaac gatcagggg
//
LOCUS       DAORNH010000211         7923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6751_length_7923_cov_2.898068, whole genome shotgun sequence.
ACCESSION   DAORNH010000211 DAORNH010000000
VERSION     DAORNH010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6751_length_7923_cov_2.898068"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="PK264_18155"
     CDS             complement(<1..1589)
                     /locus_tag="PK264_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77831.1"
                     /translation="MPNRKITITVNAASYRRWQGWVIDALRADRRLDVDVVLEAAAGH
                     DVPSSVETLLTLERMLYGRVSDHAFDRIDARETQSSSVVPRETSGPAGLALDLTLGPG
                     REGSTSPLKPLIDGDPCIGALITALLDHRLPELSLADGAGRAIPIGLVAVEEPRVLSK
                     ALDSVLCRLAATLPAIVARLALGEDDVSRAPSGAPPARRVRRSHAPHAASFAISSLAA
                     KVRDRLARILGTAPQWQLAWRKLGADNTSRLPSEIISRRDYRILPDDGRRYFGDPFGI
                     VHDGVTHVFCEELPFATMKGVISAFTLDADGTPGPVKVVLERPNHLSYPQLFVRDGHI
                     WMMPETTGSNGLELYRATRYPDQWELVARPMEGQFHDATLYDEGRRLWIFASISDRRA
                     STWDTLAIFSAERLEGVFDVVQALGCEGAPDAYTDWLDAEIQATPDDEDASASRLLQE
                     AYHPTTWVGNEAVRLARSLPEPFFLWASLPRPRWPLDPPVPWKHMYRPSRLQLPEGTA
                     LMPENGDCAVADPRDAQGHSQA"
     gene            1713..2873
                     /locus_tag="PK264_18160"
     CDS             1713..2873
                     /locus_tag="PK264_18160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD77832.1"
                     /translation="MVTLRNMPNRIPRRIVMYIHNLSGGGAERIWAVVASGLASVGHD
                     VLLVTDDEAAENKAVLDPAVRTKVLRGSHVATVAKLSRILRRERADVALAAVSGSNFK
                     LALAKAAALTRTPIVISYHGFWEHKTGWLGWLGCVSMAALSRFAARTIAVSDGLRNEL
                     VAKWHSSPDRTLRIYNPIGVPDAPEITAADLAARPELVLCVGRLIEDKDIGTVLRAFA
                     RLDRPTARLLILGEGPDRAMLETLAARLGIADRVSMPGYVLEPWVHYSKARCLVHGAK
                     QEAFGNIIVEALAYGLPVVTTACAGPSEILGRGRFGTVVPIGDDRALAAGLAAALDDP
                     GDPGPRRARAVEFALAPGIAGYETLIETVIAEHCSSRRVGVPAPQAPGSSVS"
     gene            3014..>3295
                     /locus_tag="PK264_18165"
     CDS             3014..>3295
                     /locus_tag="PK264_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRD77833.1"
                     /translation="MSAAPKVVTRFAPSPTGMLHIGNARAALFNWLYAKHTGGTFLLR
                     IEDTDRERSTPEAVATILNGLRWLGLDWDGEPTFQFARAPIHREVAEGLL"
     assembly_gap    3296..3334
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3335..4447
                     /gene="gltX"
                     /locus_tag="PK264_18170"
     CDS             <3335..4447
                     /gene="gltX"
                     /locus_tag="PK264_18170"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRD77834.1"
                     /translation="PAELAEMRAKAEAEKRPLIVESPWRDRDPKEAPAGIKPAIRIKT
                     DRGGETHVDDIVQGSVTWANKDLDDMIILRSDGAPTYNFAVVVDDHQMGVTHVIRGVD
                     HLTNTARQLQVYAALGWSPPRFGHLPLVHGPDGAKLSKRHGAQAVEDYRAMGYLPAAV
                     RNYLSRLGWSHGNDEIFSTAQAIEWFDVADVGRSPGRFDFVKLADLNGHYIRHTPDDE
                     LIGHIKAMLPHIPDGATRMAKFERIGWAKLAAAMPGLKERAKTLIELIDGAEYLISAR
                     PLTLDEKAAKLIDGEAKGHLAELATRFAARNDWTAKALEDEVRSYAEAGGFKLGKIAQ
                     PLRAALTGKSVSPPVFDVMAVLGREESLARIADQTR"
     gene            4452..5369
                     /locus_tag="PK264_18175"
     CDS             4452..5369
                     /locus_tag="PK264_18175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77835.1"
                     /translation="MTAARARPEHLALVASPGFADYRLLDSGSGRKLERFGAIVVDRP
                     EPQALWSPSLDEVAWGRAHAVFSAAGEDDEKGRWRVDKPVPDSWPIAIAAGQAKATVL
                     CRLSAMWHLGLFPEQAPHWEWMVRELCEARPERPRVLNLFGYTGAASLLAAAAGAEVT
                     HLDASKKAVQWGRENQTASRHADAPIRWIVDDARKFAAREVRRGRQYDVILVDPPKFG
                     RGPEGEVWDLFADLPALLADCVKLLAPGRAAIILTVYAIRASAIAFDQLLREHIGARG
                     GRFETGELAILEESGARAVPTSLFTRWTG"
     gene            5380..6192
                     /locus_tag="PK264_18180"
     CDS             5380..6192
                     /locus_tag="PK264_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRD77836.1"
                     /translation="MAIAETITSLQNERIKAIRALEMRKERRETGLFVAEGVSVIETA
                     RQRGIFPETLVHLAGSLDTAVARAIVTQAHAHGARILEVSAAVLAKIAAKENPQSLIG
                     VFHQRWSELPARAAMARTTTWLALEEVRDPGNLGTIIRTADAVGAAGVILVGACCDPY
                     SREAIRATMGSIFSVPLVAVTREAFLAFTKAWPGEVVGTAITATEDVRMVTWREPVLL
                     VMGSEGPGLSDGLTAAMSRLVRIPMVGRLDSLNLSVATALCLYEVRRRHLQI"
     gene            6271..7473
                     /locus_tag="PK264_18185"
     CDS             6271..7473
                     /locus_tag="PK264_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HRD77837.1"
                     /translation="MSENPTTVATRRRRLGGRRFLLSLCIVGAAVCLALGVSQPIIKV
                     TKYVFFTTEHSLISTVATLIKSGQTFLGVTVLVFSIVLPVLKLLYLVAVSVLPSSELQ
                     RQKRRLIALEWLGKWSMHDVLVLSLTIFFIKSQGVWDAASLKGVYFFTAAVILMILAY
                     AFLRYDAVAPAVVHVEPVGRPSPVRNFALSFLIILATVFFALGVILPVIRFTTVYVWT
                     NEHSLSTIIWALYSNEEYFLCVVIFIFSIFFPFLKLFYLLTLMTSPDLSPEFRRRSAS
                     TMEWLGRYSMTDVMVLALMIFYFNSSDYTEASVLPGAYFFAASVLMTMLAYGWANSVT
                     PSVAYRASEPAEAVGAHANASGVAALPSGGPVSPAPPPSINTSAAKLPTPSAFPVRPK
                     QGVRADGT"
     gene            7660..>7923
                     /locus_tag="PK264_18190"
     CDS             7660..>7923
                     /locus_tag="PK264_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77838.1"
                     /translation="MARPGAAADAPGIPGISLATVSADDFDRLTELATGVDAAVISVE
                     GSSARQALAILNALGVRRVVLQGGGAEAIAALAEHARAGLGMSV"
BASE COUNT         1269 a         2639 c         2643 g         1333 t
ORIGIN      
        1 gcctggctgt ggccttgagc gtcacggggg tccgcaacag cgcagtcccc attctccggc
       61 atcagcgccg tgccctcggg caattggagg cgcgatggac ggtacatgtg tttccacggc
      121 acgggcggat ccaggggcca gcggggccgg ggcaggctgg cccacagaaa aaagggctcg
      181 ggcagggagc gggcgaggcg cacggcctca ttgcccaccc aggtggtcgg gtgataggcc
      241 tcttgcagaa gtcgcgaggc cgatgcgtct tcgtcatcgg gcgtagcctg aatctcggcg
      301 tccagccagt ccgtgtacgc atcgggcgct ccttcgcatc ccagggcctg aacgacatcg
      361 aagacccctt cgagccgctc ggcggaaaag atcgcgagcg tgtcccaggt cgaggcgcgc
      421 cggtcggaga tgctggcaaa gatccagagg cgacggcctt cgtcgtagag cgtcgcatcg
      481 tggaactggc cctccatcgg gcgcgccaca agttcccact ggtctgggta gcgcgtcgcg
      541 cgatagagtt cgaggccgtt gctgccggtc gtctcgggca tcatccagat atggccatcg
      601 cggacgaaga gctgcgggta ggagagatgg ttcggccgct cgagcacgac tttcactggg
      661 ccgggcgtcc cgtctgcatc tagcgtgaag gccgagatca cccctttcat cgtggcgaac
      721 ggcagctcct cgcagaagac atgcgtgacg ccgtcatgaa cgatgccgaa gggatcgccg
      781 aagtagcggc gaccgtcgtc cgggagaatg cggtagtcgc gccggctgat gatctcgctt
      841 ggcaggcgcg aggtgttgtc ggcaccgagc ttgcgccagg cgagctgcca ttgcggcgct
      901 gtgccgagga ttcgcgcgag gcgatcgcgc actttggcgg ccagactcga gatggcgaac
      961 gaggcggcgt gcggcgcgtg cgacctgcga actcgacgcg cgggcggtgc gccgctcggg
     1021 gctctggata cgtcgtcctc gccgagagcg aggcgtgcga cgatcgcggg cagagtggct
     1081 gcgagccggc agagcacgct atcgagcgcc ttggagagca cgcgcggctc ctcgacggcg
     1141 acgaggccga tcgggatcgc ccggcctgcg ccgtcggcaa ggctcaactc gggcaggcga
     1201 tgatcaagta gcgcggtgat caatgccccg atacacgggt cgccatcgat caaaggcttg
     1261 agagggctgg tcgatccttc ccgtccgggt ccgagtgtga ggtcgagcgc aagcccggcc
     1321 gggcccgagg tttcccgcgg cacaactgat gaagactgcg tctcgcgagc atcgatgcga
     1381 tcgaacgcat gatcgctcac ccgaccataa agcatccgct cgagcgtcag cagcgtctcg
     1441 accgacgagg ggacgtcatg gcccgcggcc gcctcgagca ccacgtcgac gtcgaggcgg
     1501 cgatctgctc gcagcgcatc gatcacccag ccctgccagc gcctgtagct cgcagcgtta
     1561 acagtgatgg tgattttccg gttgggcacg gagctttcgc agtctaccag catgggttaa
     1621 cggacaacga cgcgggacag gcacgcaagg tgcataccat cgggggaact gtcgtgagca
     1681 gtggcgctgg tcgaattgtc gtgaaggacg ccatggtaac cctccggaac atgccgaacc
     1741 gcatcccgcg gcgaatcgtc atgtacattc ataacctgtc cggcggcggc gccgagcgga
     1801 tctgggccgt ggtcgcctcg ggcctcgcga gcgtcggtca tgacgtcctg ctcgtgacgg
     1861 acgatgaggc ggcagagaac aaggctgtgc tcgatccggc ggtgcggacc aaggtgctgc
     1921 gcggcagcca cgtggcgact gtcgctaagc tttcgcgcat cctgcgccgg gagcgcgctg
     1981 acgtggcgct agccgccgtc tccggctcga atttcaagct cgccctcgcc aaggctgcgg
     2041 cgctgacgcg gacgccgatc gtgatctcgt accatggctt ctgggagcac aagaccggct
     2101 ggctcggctg gctaggctgt gtatcgatgg ccgctctctc ccgtttcgcg gcccgcacca
     2161 tcgccgtgtc cgacggcttg cggaacgagc tagtggccaa atggcactcg tcaccggacc
     2221 gcacgctcag gatctacaat ccgattggcg tacctgatgc gccggagatc acggccgccg
     2281 acctcgcggc gcggccggag cttgtcctct gcgtcggccg actgattgag gacaaggaca
     2341 tcggaacagt gctcagggcc ttcgcgcggc tcgatcggcc gaccgcgcgg ctgctgatcc
     2401 tcggcgaggg tcccgatcgg gcgatgctcg agacgctggc cgcacgcctc ggcatcgccg
     2461 atcgcgtgag catgccgggc tacgtgctcg aaccgtgggt ccactattcg aaggcgcgct
     2521 gcctcgtgca tggcgccaag caggaagcat tcggcaacat cattgtcgag gcgctggcct
     2581 acggcctccc tgtcgtgacg acggcgtgcg cgggtccgag cgaaatcctc ggccgcggtc
     2641 gctttggtac ggtcgtgccg atcggggacg atcgggcgct ggcggcgggc ctcgcagcag
     2701 cgctcgacga cccgggtgat ccgggtccac gccgcgcacg agcggtcgag ttcgcgctgg
     2761 cgccaggcat tgccggctac gaaacactga tcgaaacggt cattgcggag cactgctcga
     2821 gccggcgggt gggcgtgccg gcgccccagg cccccggcag ctcggtgtcc tgagcagacc
     2881 gtccaagtga cggttctcgg acatcggcca agctcgaacg gcgattgcga cccgagggac
     2941 tgattcggca aaagggcgtt gccagcgctt cccgcgcctc gtacatccgc gcaatcgtcg
     3001 aacgaggctc tccatgtccg ccgcacccaa ggtcgttacc cgcttcgcgc cgtccccgac
     3061 gggcatgctg cacatcggca atgcccgagc agcactcttc aactggctct acgcgaagca
     3121 caccggcggc acgttcctgc tgcgcatcga ggacactgac cgcgagcgct cgacgcccga
     3181 agccgtcgcg acgatcctca atggcctgcg ctggctcggc ctagactggg acggcgagcc
     3241 gacgttccag ttcgcgcgtg cgccgatcca ccgggaggtc gccgaaggac tgctcnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccgcc gaactcgccg agatgcgcgc
     3361 caaggccgag gccgagaagc gcccgctgat cgtcgagagc ccctggcgcg accgcgatcc
     3421 gaaggaggcg ccggccggca tcaagccggc gatccgcatc aagaccgacc ggggtggcga
     3481 gacgcatgtc gacgatatcg tccagggttc cgtgacgtgg gccaacaagg acctcgacga
     3541 catgatcatc ctgcgctcgg acggcgcgcc gacctacaat ttcgccgtcg tcgtcgatga
     3601 tcatcagatg ggcgtgacgc acgtcatccg cggcgtcgat cacctgacta acacggcgcg
     3661 ccagctccag gtctatgcgg cgctcggctg gtcgccgcca cgcttcggcc atctgccgct
     3721 cgtccatggt cccgacggcg ccaagctctc taagcggcat ggcgcgcaag cggtcgagga
     3781 ctaccgcgcc atgggctatc tgccggcggc ggtgcgcaac tatctctcgc gcctcggctg
     3841 gagccacggc aacgacgaga tcttctcgac cgcgcaggcg atcgaatggt tcgacgtcgc
     3901 cgatgtcgga cgctcgcccg gccgcttcga tttcgtgaag ctcgccgacc tcaacggcca
     3961 ctacatccgc cacactcccg acgacgagct catcgggcac atcaaggcga tgctgccgca
     4021 catccctgac ggcgcgaccc gcatggcgaa gttcgagcgg atcggctggg cgaagctcgc
     4081 ggccgccatg ccgggcctca aggagcgcgc gaagacgctg atcgagctca tcgacggcgc
     4141 cgagtacctg atctcggccc gaccgctgac tctcgacgag aaggcggcaa agctcatcga
     4201 tggtgaggcg aaggggcatc tcgccgagct cgccacacgc ttcgcggcgc ggaacgactg
     4261 gaccgcgaag gcgctcgagg acgaggtgcg ctcctatgcc gaggcgggcg gcttcaagct
     4321 cggcaagatc gcgcagccgc tcagggcggc tctcacgggg aagtccgtct ccccgcccgt
     4381 cttcgacgtg atggcggtgc tcggccgcga ggagagcctc gcacgcatcg ccgatcagac
     4441 gcgctgaagc catgacggcg gcgcgcgcac ggcctgagca tctggcgctc gtcgcctctc
     4501 cgggcttcgc cgattatcgc cttctcgaca gcggcagcgg ccgcaagctc gagcgcttcg
     4561 gggcaatcgt cgtcgatcgt ccggagccac aggcgctctg gtcaccgtcg ctcgatgaag
     4621 ttgcctgggg gcgggcgcac gccgtctttt cggccgcggg cgaagacgac gagaaggggc
     4681 gctggcgcgt cgacaagcct gtccccgaca gctggccgat cgccatcgcg gccggccagg
     4741 cgaaggcgac agtcctttgc cgcctgtccg cgatgtggca cctcgggctc tttcccgagc
     4801 aggcgccgca ctgggagtgg atggtgcgcg aactctgcga ggcgcggccg gagcgaccgc
     4861 gcgtcctcaa tctttttggc tacaccggcg cggcaagcct cctcgcagca gcagcaggcg
     4921 ccgaggtcac gcacctcgat gcctcgaaga aggccgtgca gtggggtcgc gagaaccaga
     4981 ccgcatccag gcacgctgat gcgccgatcc gctggatcgt cgacgatgcg cggaagttcg
     5041 ccgcccgcga ggtccggcgc ggtcgacagt acgacgtcat cctggtcgat ccgccgaagt
     5101 tcggccgcgg ccccgagggc gaggtgtggg atctgttcgc cgatctgccg gcgctgctcg
     5161 cggactgcgt gaagctcctt gccccggggc gggcggcgat catcctcacg gtctatgcga
     5221 tccgcgcctc ggccatcgcc ttcgaccagc tgctgcgcga gcacatcggc gcgcgcggcg
     5281 gtcgcttcga gacgggtgag ctcgcgatcc tcgaagagag cggcgcgcgc gccgtgccga
     5341 cgtcgctctt cacgcgctgg acggggtgaa ggggccgcca tggcaatcgc tgagacgatc
     5401 acaagcctcc agaacgagcg catcaaggcg atccgagcgc tcgagatgcg caaggagcgg
     5461 cgcgagacgg gcctgttcgt cgccgagggc gtctcggtga tcgagaccgc gcggcagcgg
     5521 ggcatctttc ccgagacgct cgtgcatctc gcaggcagcc tcgacacggc cgtcgcgcgc
     5581 gcgatcgtca cgcaagccca cgcccacggc gcgcgtatcc tcgaggtgag cgccgctgtg
     5641 ctcgccaaga tcgcagcaaa ggagaacccg caatccctga tcggcgtctt ccaccagcgc
     5701 tggtcggagc tgcctgcgcg agctgcgatg gctcgcacaa cgacttggct tgcgctcgag
     5761 gaggtgcgcg atcccggcaa tctcggcacg atcatccgca ccgccgatgc ggttggcgcg
     5821 gcgggcgtca tcctggtcgg cgcatgctgc gatccctact cgcgcgaggc catacgcgcg
     5881 accatgggct cgatcttctc ggtgccgctc gttgccgtga cgcgcgaggc cttcctcgcc
     5941 ttcaccaagg catggccggg cgaggtcgtc ggcacggcaa tcacggccac cgaagacgtg
     6001 cggatggtca cgtggcgcga gccagtattg ctcgtcatgg gctcggaagg ccccgggctt
     6061 tcggacgggc tgacggcggc gatgtcacgc ctcgtgcgca tccctatggt cggccggctc
     6121 gacagcctca atctctccgt ggcgacggcg ctctgcctct atgaggtacg gcgccggcac
     6181 ctgcagattt gatcgaagcc ggtgtgacca tattgcggcc tcggagcgat cgcataggat
     6241 cggtgctgac acggccaggg cagttcgcgc atgtccgaaa acccgacgac agtagcaacg
     6301 cgccgccggc ggctcggcgg gcggcgcttt ctactgagcc tttgcatcgt cggcgcggcg
     6361 gtttgcctgg cgctcggcgt cagccagccg atcatcaagg tgacgaagta cgtcttcttc
     6421 acgaccgagc attcgctcat ttcgacggtc gcgacactca tcaaatcggg ccagacgttc
     6481 ctcggcgtca cggtcctcgt attctcgatc gtgctgccgg tcctgaagct cctctacctc
     6541 gtcgccgtct ccgtgctgcc gtcgagcgag ctgcagcgcc agaagcgccg gctcatcgcg
     6601 ctcgaatggc tcggcaagtg gtcgatgcac gacgtgctcg tgctctcgct caccatcttc
     6661 ttcatcaagt cacagggggt gtgggacgcg gcgagcctca agggcgtcta cttcttcacg
     6721 gccgccgtca tcctgatgat cctcgcctat gcgttcctgc gctacgatgc agtggcgccc
     6781 gcggtcgtcc acgtcgagcc agttggccgg ccgtcgcccg tgcgcaactt cgcgttgagc
     6841 tttctcatca tcctggcgac cgtgttcttc gcgctcggcg tgatcctccc agtgatccgt
     6901 ttcaccaccg tctatgtgtg gacgaacgag cattcgctct cgacgatcat ctgggcgctc
     6961 tacagcaacg aggagtactt cctctgcgtc gtcattttca tattctcgat tttcttcccg
     7021 ttcctcaagc tcttctactt gctcacattg atgacatcgc cggacttgag ccccgaattc
     7081 cgccgccgct cggcctcgac catggaatgg ctcggtcgat actccatgac ggacgtgatg
     7141 gtgctcgcct tgatgatctt ctacttcaac tcctcagact acaccgaagc gtcagttctg
     7201 ccgggcgcct atttcttcgc ggcctcggtg ctgatgacga tgctcgccta cggctgggcc
     7261 aactcggtga cgccgtcagt ggcctacaga gcctctgagc ccgccgaggc cgtgggcgcc
     7321 catgccaacg cctcaggtgt agcagcgctg ccatcgggtg gccccgtcag tccggcgccg
     7381 ccgccgagta tcaacacgtc tgcagcgaag ctgccgacac catccgcatt ccccgttcgg
     7441 cccaagcagg gcgtgcgcgc cgacggcaca tgagcctgat gagcgcggaa ctgtggattg
     7501 gactgccacg gaggcggtaa gcctcgaatg atgctatctg catgcctccg ccgcggccat
     7561 gatcggccgt gacgcatcca gatgctgcga gcctgcggcg gcacgcggca catgacttga
     7621 ggagcggcca tgccgacgcg acatgtgaga gtccttcatg tggcccgccc cggcgccgcg
     7681 gcagacgcgc ccggcattcc cggtatttca ctggcgaccg tttcggccga tgatttcgac
     7741 cgcttgacgg aactcgcgac cggcgtcgat gcggcggtca tcagcgtcga ggggtcgtct
     7801 gcgcggcagg cgctggcgat cctgaacgcg ctcggcgtgc gccgcgtggt gctgcagggt
     7861 ggcggagccg aggcgatcgc cgcgctcgcc gagcatgccc gtgcggggct cggcatgtcg
     7921 gtg
//
LOCUS       DAORNH010000212         7915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6757_length_7915_cov_2.791985, whole genome shotgun sequence.
ACCESSION   DAORNH010000212 DAORNH010000000
VERSION     DAORNH010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6757_length_7915_cov_2.791985"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="PK264_18195"
     CDS             <1..154
                     /locus_tag="PK264_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRD77839.1"
                     /translation="HTDGSLGYFQNSFSVYDREGEPCANTSCRGIVRRNMQGGRSTFA
                     CPVCQR"
     gene            261..1034
                     /locus_tag="PK264_18200"
     CDS             261..1034
                     /locus_tag="PK264_18200"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HRD77840.1"
                     /translation="MAYTSIIADTRGRVGIVTLNRPNALNALNAALIAELGQAIDGFE
                     ADEDIGCIVITGSDKAFAAGADIKEMQSKSYMQAYKEDFIGSWDRVARCRKPVIAAVA
                     GYALGGGCELAMMCDFIIAADTAKFGQPEIKLGVMPGAGGTQRLARFVGKAKAMDMCL
                     TGRNMDADEAHKCGLVSRIVAADKLMEEALKAADIIAGMSLPALMMTKESVNRAFETT
                     LGEGIRFERRVFHSMFAMDDQKEGMKAFAEKRKPNFQHK"
     gene            complement(1276..2637)
                     /gene="pcaB"
                     /locus_tag="PK264_18205"
     CDS             complement(1276..2637)
                     /gene="pcaB"
                     /locus_tag="PK264_18205"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HRD77841.1"
                     /translation="MPSSMFDSGIFRDCFGTPAMRAVFSDEALVQRYVEVEVALARAE
                     GRLGVIPNEAADGIAKAAAPGSIDIARLKAETDNVGYPIVGIVHALQKQCGEAGRYLH
                     WGATTQDIMDTATVLQIRAALDLVAADLEAIDSALAGLARTHRDTVMAGRTHLQHALP
                     VTFGFKAAVWLSMIRRHRERLTQARPRVEVVEFAGAAGTLASLGTRGLEVQAGLAREL
                     GLAEPTATWHAARDSVAEVVALLGLVTGSLAKIATDVMLMMQTEIGEAFEPFVKGRGS
                     SSTMPQKRNPISCELIVALAKIVRSNVGLMLDGMAADHERATGPWHLEWVALPEAFIA
                     AAGALGQARFMLSGLIVDAPRMRRNLDMTGGLIVAEAAMMALAPKMGRGAAHDVVYAA
                     CRRALDKGTTLLDQLAVDAAVTAHFDKAALVRVVDPASYLGVAGQMVDRVLGDGRTGG
                     SHH"
     gene            2753..3079
                     /locus_tag="PK264_18210"
     CDS             2753..3079
                     /locus_tag="PK264_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HRD77842.1"
                     /translation="MVYELRIYHCVPGRLPALLKRFETITLKIWERHGIRQAGFWTVL
                     VGESNQALYYLLAWDSLADREKKWNAFMADPEWIAKRAETEKDGAIVARVDNSMLAPT
                     AFSSVK"
     gene            complement(3116..3841)
                     /locus_tag="PK264_18215"
     CDS             complement(3116..3841)
                     /locus_tag="PK264_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRD77843.1"
                     /translation="MSRTLDHLLDRNVAWAAEKRHDDPEFFRRMAEQQQPSYLWIGCS
                     DSRVTANDVLGLDPGEVFVHRNIANVAHTSDLNILSVLEFAIEHLQVRHIIVCGHYGC
                     GGIERALSVERGAMVDHWLQPITMLYRKHRAFFATITDRARLVDRMCEINIEMQVRRI
                     ATMPLVENAWSRGQELNLHGWIYGIGDGLLRDLGPHVASIAERDALPSIDQCVSEPVE
                     AMTAKRRHAIEAFSGGPRTPCSC"
     assembly_gap    4138..4147
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4300..5166)
                     /locus_tag="PK264_18220"
     CDS             complement(4300..5166)
                     /locus_tag="PK264_18220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD77844.1"
                     /translation="MTTVLDTHAVAAAGCAAKPSRLSRPKPISVNGVVIAHAAISRET
                     QNHPAEKPLDAWMAAARALVIRELLLQEACRLGLTPKPQSDEEGRRETDEEALMRELT
                     EREIVTPTPDEATCRRVYELQRARFRSSDLYAARHILLAAAPDDAKRRQEARTVAEAL
                     LVEIGARPESFARLAAAHSACPSSAHGGNLGQISRGQTVPEFEAALATLELPGIVETR
                     FGFHVVIVDQHVFGRELPFEVVRDRIAGWLAERSRHAAIRQFIAMLASRAEVTGLDLG
                     VGNSALDQVMAS"
     gene            complement(5171..5902)
                     /gene="narI"
                     /locus_tag="PK264_18225"
     CDS             complement(5171..5902)
                     /gene="narI"
                     /locus_tag="PK264_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HRD77845.1"
                     /translation="MRDALFHILFVWYPYVCLSAFIFGSIIRFDREPYTWKASSSQML
                     RKRQLFWGSNLFHFGILFLLVGHTVGLLTPKAIYTLFVTPAQKQLLAIIAGGIAGALC
                     FIGLSLLLHRRLFDARIRQTSTYMDLAVLIILWVQLVLGLVTLPFSLGHTDGSVMIQL
                     SNWAQGILLVQPVDAKTLQGLDWPYLVHLVLGMTIFLLFPFSRLVHIWSAPVWYLGRK
                     GYQVVRTRRPLGTASKEGSYTQAAE"
     gene            complement(5913..6665)
                     /gene="narJ"
                     /locus_tag="PK264_18230"
     CDS             complement(5913..6665)
                     /gene="narJ"
                     /locus_tag="PK264_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HRD77846.1"
                     /translation="MMKTLKVLSALLTYPTPELAAAAGELRHAVTAEGLLRGPVRDGV
                     LALIDDLACRDIYEAQERFILLFDRTRSLSLHLFEHVHGESRDRGQAMVDLIKVYEDK
                     GYTPTTSELPDFLPLFLEFASTEEPKAALELIAQPGHVFAALQERLRRRSSPYAALFE
                     ALVVLGKAKIDPAALAALRAEPDPEPDDLEALDRAWAEEEVTFGPGAQSDCGVDSLTA
                     KIRQGRRPAPGLDAPRDGRPRVAITTPSTPRA"
     gene            complement(6662..>7915)
                     /gene="narH"
                     /locus_tag="PK264_18235"
     CDS             complement(6662..>7915)
                     /gene="narH"
                     /locus_tag="PK264_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HRD77847.1"
                     /translation="PNMPEIDDYYEPFTFDYEHLQKAPESEAFPTARPRSLITGERME
                     KIEWGPNWEEILGGEFAKRSKDVNFDGVQKEIYGQFENTFMMYLPRLCEHCLNPACVA
                     SCPSGAIYKREEDGIVLIDQEKCRGWRMCVSGCPYKKIYFNWSSGKSEKCILCYPRIE
                     AGQPTVCSETCVGRIRYLGVILYDADQIEKAASTVDEKDLYEQQLSVFLDPANPLVIE
                     RARADGVPDAWLEAARRSPIWKMAMEWKVAFPLHPEYRTLPMVWYVPPLSPITAAAEA
                     GQVASDAGMPDVKSLRIPVRYLANLLTAGDERPVVTGLQRMLAMRAYMRAKTVDGVID
                     EAIAKRVGLTAQQIEDMYHIMAIANYEDRFVIPTSHREVTQDAYELRGECGFSFGDGC
                     LPSDTRPNLFGGVKVKNDKPRMGAY"
BASE COUNT         1386 a         2587 c         2637 g         1295 t
ORIGIN      
        1 acacacggac gggtcactcg gctatttcca aaacagtttt tccgtttacg atagggaggg
       61 agagccttgc gcaaacacct cctgccgggg cattgtgcgc cgcaatatgc aggggggccg
      121 ctcgaccttc gcctgtccgg tctgccagcg gtagggatcg gacgggggaa gcgtgcaccc
      181 cgtggacatc ggatctgccg tgcccgcccg ggctcgcacg gctcggataa cagaactctc
      241 aagcacccaa ggagtgggcc atggcctaca caagcatcat cgccgacacg cgggggcgcg
      301 tcggcatcgt caccctcaac cggccgaacg cgcttaatgc gctcaatgct gcgctcatcg
      361 ccgagctcgg tcaggcgatc gacggcttcg aggccgacga ggacatcggc tgtattgtga
      421 tcaccggcag cgacaaggcc ttcgcggccg gcgccgacat caaggagatg cagtcgaaga
      481 gttatatgca ggcctacaag gaggacttca tcggcagctg ggaccgcgtc gcccgctgcc
      541 ggaagcccgt gatcgcggca gttgcgggct acgcgctcgg cggcggctgc gagctcgcca
      601 tgatgtgcga tttcatcatc gccgccgata ccgcgaaatt cggccagccc gagatcaagc
      661 ttggcgtcat gccgggcgcc ggcggcacgc agcgcctcgc ccgtttcgtt ggcaaggcca
      721 aggccatgga catgtgcctg acgggccgca acatggatgc ggacgaagcg cacaagtgcg
      781 gcctcgtctc gcgcatcgtc gccgccgaca agctgatgga ggaggcgctc aaggccgccg
      841 acattattgc aggcatgtcg ctcccggccc tcatgatgac caaggagagc gtgaaccgcg
      901 ccttcgagac cacgctcggc gagggaatcc gcttcgaacg ccgcgtgttc cactccatgt
      961 tcgctatgga cgaccagaag gaaggcatga aggccttcgc cgagaagcgg aagccgaact
     1021 tccagcacaa gtgacgactg gcgccggtcc actggtgact gatcgatccg tccggcgccc
     1081 cgccatgtct ctggcgctgc gccggacgcg catagcagtt ccggatgtga ccacgagccc
     1141 gaggcctctc aaagccgtct gggaccgccg gctcggcccg gatatggatc ggggcagcat
     1201 gacttcgccc tctcgccgcc gcaggactgc gctgagtgtg gcgaggcccg acgggcgctg
     1261 cccggggtcg cttgctcaat ggtggctgcc cccggtcctc ccgtcgccga ggacgcgatc
     1321 gaccatctgc cctgcaacgc cgagatagct cgcgggatcg accacgcgca caagcgctgc
     1381 cttgtcgaaa tgcgccgtca cggcggcatc gaccgcaagt tgatcgagaa gcgtcgtacc
     1441 cttgtcgagc gcgcgccggc aggccgcata gaccacatcg tgtgcagcac cccgtcccat
     1501 cttcggcgcg agcgccatca tcgcagcctc ggcgacgatg aggccgccgg tcatgtcgag
     1561 gttcctccgc atgcggggcg catcgacgat cagccccgag agcatgaagc gggcctgacc
     1621 gagcgcaccg gccgccgcga tgaacgcctc cggcagtgct acccactcga gatgccaggg
     1681 gccggtcgct cgctcgtggt cggccgccat cccgtcgagc atcaggccga cattcgagcg
     1741 cacgatcttc gctagcgcga cgatcagctc gcaggagatc ggatttcgct tctgcggcat
     1801 cgtcgatgac gagccgcgcc ccttcacaaa cggctcgaag gcctcgccga tctcggtctg
     1861 catcatcagc atgacgtcgg tcgcgatctt ggcgagcgag ccggtaacga gcccgagcag
     1921 cgccaccacc tcggcgacgg aatcgcgcgc ggcatgccag gtcgccgtcg gctctgcgag
     1981 gccgagctca cgcgcgagac ccgcctgcac ctcgaggccg cgcgtgccga gggaggcgag
     2041 tgttccagct gcccctgcaa actcgacgac ctcgacgcgc gggcgcgcct gcgtaagccg
     2101 ctcgcggtgc cggcggatca tggagagcca gaccgccgcc ttgaagccga atgtaacggg
     2161 gagcgcatgc tggagatgcg tgcgccccgc catcaccgtg tcgcgatgcg tccgcgcgag
     2221 gccggcgagt gcgctgtcga tcgcctccag gtcggccgcg acgaggtcga gggccgcccg
     2281 gatctgcagc accgtcgccg tgtccatgat gtcctgcgtg gtcgcccccc agtggagata
     2341 gcgccccgct tcgccgcact gcttctgcag tgcgtgcacg atgccgacga tcgggtagcc
     2401 gacattgtcg gtttccgctt tcagcctcgc gatatcgatg ctgccagggg ccgccgcctt
     2461 ggcaatgcca tccgccgcct cgttagggat cacgccgagc cgcccctccg cccgggcgag
     2521 cgcgacctcg acctcgacgt agcgttgaac cagcgcctcg tccgagaaga ccgcccgcat
     2581 ggccggcgtt ccgaagcagt cccgaaagat tccagaatcg aacatcgacg acggcatggc
     2641 gcgctccctt gatctcgtcg aggcgtagct ggcatgcgcc ccggcgacaa gggctcgcca
     2701 gcggaagggg cggcagctat cgtcaggcgt ccttcaccaa cggagacgcg acatggtcta
     2761 cgagctgcgc atctatcact gtgtgccggg ccgactgccg gcacttctaa agcgcttcga
     2821 gacgatcacg ctcaagatct gggagcggca cggcattagg caggcgggct tctggacggt
     2881 actggtcggc gaatccaacc aggcactcta ttatctgctc gcctgggata gcctcgctga
     2941 ccgcgagaag aagtggaatg ccttcatggc cgaccccgag tggatcgcca agcgcgccga
     3001 gaccgagaag gacggcgcca tcgtcgcccg tgtcgacaac agcatgctcg cgccgacggc
     3061 cttttcgtcg gtgaagtaga acgaaggggg cgaagtcatc gccctctcat cttcgtcaac
     3121 acgagcaggg cgtgcgtggc ccgccactaa acgcctcgat cgcgtgacgg cgcttcgccg
     3181 tcattgcctc gacaggctcg ctgacgcact ggtcgatgga cggcagcgca tcgcgctcgg
     3241 cgatcgaggc gacgtgcggt ccgaggtcgc gcaagagccc gtcgccgatc ccgtagatcc
     3301 agccgtgcag attcagctcc tgcccgcgcg accaggcgtt ctcgacgagc ggcatggtgg
     3361 cgatgcggcg cacctgcatc tcgatgttga tctcgcacat ccggtcgacg agtcgcgccc
     3421 gatccgtgat cgtcgcgaag aaggcgcggt gcttgcgata gagcatcgtg atgggctgca
     3481 gccagtgatc gaccatggca ccgcgctcaa ccgacagtgc gcgctcgatc cctccgcagc
     3541 cgtagtggcc acagacgatt atgtggcgaa cctggaggtg ctcgatcgcg aactcgagca
     3601 cggagagaat gttgagatcg ctggtgtgcg cgacgttggc gatgttgcga tgaacgaaga
     3661 cctcgcccgg atcgaggccg agcacgtcgt ttgcggtgac gcgactgtcc gagcagccga
     3721 tccaaaggta gctcggctgc tgctgctcgg ccatccggcg gaaaaactcc gggtcgtcgt
     3781 ggcgcttctc cgctgcccag gcaacgttgc ggtcgaggag atgatcgagg gtgcgggaca
     3841 tgcgggctat cccttccgct acgtcgagat aaagttgtgg ggctctgccc cgcgccccgc
     3901 caaaggccgt cgatgcggag catcgctacg cgatgacggc ctttggaagc cgatcatcaa
     3961 ggagagatca gccgcgacag gagcagctgg aggttgccga gttcaggggc tccgaggtaa
     4021 cgcgcgcgag cgcccagcgc acaaggcgtt gcagggcagc tgcacccggg gcctctgcgc
     4081 gctcgacggc cgcgatgagc gattgccagt cctcgtcacg ggccaagttg gcgaaacnnn
     4141 nnnnnnntgc ggcacttgct gcaacgaact cgccgggcgt ctcaccgtag cgctctgcca
     4201 tcgccgccac ctcggcgtag agcgggaggt cgcccagcgc tgcgattgcc tccgcggcgc
     4261 tctcttcgct ctcgaggcgg gcgatcagcg tcccaagcat catgacgcca tcacctggtc
     4321 gagtgccgag ttgccgacgc cgaggtcgag gcccgtgacc tcggcacggc tcgcgagcat
     4381 cgcgatgaac tggcgaatcg ccgcgtggcg cgagcgctcg gcgagccagc cggcgatccg
     4441 atcgcgcacg acctcgaacg gcagctcgcg accgaagacg tgctgatcga cgatgacgac
     4501 atggaatccg aagcgcgtct cgacgatgcc cggtaattcg agcgtcgcga gtgcagcctc
     4561 gaactcaggc accgtctggc cgcggctgat ctggccgaga tttccgccgt gcgcggacga
     4621 cggacaggca gagtgggccg ccgcgagccg cgcaaagctt tccggccggg cgccgatctc
     4681 gaccagcagc gcctctgcca cggtgcgcgc ctcctgccgt cgcttcgcgt cgtcaggcgc
     4741 ggcggcgagc agaatgtggc gtgcggcgta gagatccgag gatcggaagc gcgcacgctg
     4801 gagttcgtag acgcgccgac acgtcgcctc gtcgggtgtc ggcgtcacga tctcgcgttc
     4861 cgtaagctct cgcatgagcg cctcctcgtc ggtctcgcgg cggccctcct cgtcgctctg
     4921 gggcttgggc gtcagcccga gccgacacgc ctcctgcagc aacagctcgc ggatgacgag
     4981 cgcgcgcgcc gccgccatcc acgcatcgag cggcttctcc gccggatggt tctgcgtctc
     5041 gcgcgagatc gccgcgtgag caatcaccac gccgttgacg ctgatcggct tcggcctgga
     5101 gaggcgggag ggcttggcgg cacagccggc agctgcaacg gcatgagtat cgagcacggt
     5161 ggtcattcga ctactccgcc gcctgagtgt agctgccttc cttcgaggcg gttccgagcg
     5221 gccggcgcgt gcggacgacc tgatagccct tccgcccgag ataccagacc ggcgccgacc
     5281 agatgtgcac gagccgcgag aacgggaaga gcaggaagat ggtcatgccg aggacgaggt
     5341 ggacgaggta gggccaatcg aggccctgca gcgtcttggc gtcgaccggc tgcacaagca
     5401 ggataccctg cgcccaattc gagagctgga tcatcaccga gccgtcggtg tggccgagcg
     5461 agaacggcag cgtgacgagg ccgagcacca gctgcaccca gaggatgatc agcacggcaa
     5521 gatccatgta cgtcgacgtc tgccggatgc gcgcatcgaa caggcggcga tgcagcaaca
     5581 gcgagagccc gatgaagcag agggcgccgg cgatcccgcc cgcgatgatg gcgagcagct
     5641 gcttttgcgc cggggtcacg aagagcgtgt agatggcctt cggtgtcagc aggccgaccg
     5701 tatggccgac aagcaggaag aggatgccga aatggaagag gttcgagccc cagaagagct
     5761 ggcgcttccg gagcatctgg ctcgacgagg ccttccaggt gtagggctcg cggtcgaagc
     5821 ggatgatgct gccgaagatg aaagccgaga ggcacacgta cggataccag acgaagagaa
     5881 tatggaagag tgcgtcgcgc atggtgctct cctcaggcgc ggggcgttga cggggtggtg
     5941 atggcaacgc gcgggcggcc atcgcgtggc gcatcgaggc ccggcgcagg ccggcggccc
     6001 tggcggatct tggcggtgag gctgtcgaca ccgcagtcgc tttgcgcacc cggaccgaag
     6061 gtcacctcct cctcggccca ggcccggtcg agcgcctcga ggtcgtcggg ctcggggtcc
     6121 ggttcggcac gcagagccgc caatgcggcc ggatcgatct tcgccttgcc gagcaccacg
     6181 agggcctcga aaagagcagc atacggtgac gatcggcgcc tgagccgctc ctgaagtgcc
     6241 gcgaaaacgt gtcccggctg cgcgatcagc tcgagcgcgg ccttgggttc ctcggtcgag
     6301 gcgaactcga ggaaaagcgg caggaagtca ggcaactccg aggttgtcgg cgtatacccc
     6361 ttgtcctcgt agacctttat gaggtcgacc atcgcctggc cgcggtcacg gctctcgcca
     6421 tgcacgtgct cgaagagatg gagcgagagt gagcgcgtgc gatcgaagag caggatgaac
     6481 cgctcctgcg cctcgtagat gtcgcggcac gcgagatcgt cgatgagggc gagaacgccg
     6541 tcgcgcaccg ggccacggag caggccctct gcggtcacgg cgtgcctgag ctcgccggcc
     6601 gccgctgcaa gctcgggcgt cggataggtg aggagcgcgg agagcacctt gagcgttttc
     6661 atcagtaggc tcccatgcgc ggcttgtcgt tcttcacctt gacgccgccg aacaggtttg
     6721 gccgcgtgtc gctcggcagg cagccgtcac cgaacgagaa gccgcattcg ccgcgcagct
     6781 cataggcgtc ctgcgtcacc tcgcggtggc tcgttgggat gacgaagcgg tcctcgtaat
     6841 tggcgatcgc catgatgtgg tacatgtcct cgatctgctg cgccgtgaga ccgacgcgct
     6901 tggcgatggc ctcgtcgatc acgccatcga cggtcttcgc ccgcatgtag gcgcgcatcg
     6961 cgagcatgcg ctgcaatccc gtcacgacgg ggcgctcgtc gcccgcggtc aggagattgg
     7021 cgagatagcg cacgggaatg cgcaacgatt tcacgtcggg catgccggca tcgctcgcca
     7081 cctggccggc ttccgccgcc gccgtgatcg gcgagagcgg cggcacgtac cagaccatcg
     7141 gcagcgtccg gtactcggga tgcagcggga aggcgacctt ccactccatc gccatcttcc
     7201 atatcggcga gcggcgagcg gcttcgagcc aggcgtccgg cacgccgtcg gcccgcgccc
     7261 gctcgatcac caacgggttg gcgggatcga ggaacacgga gagctgctgc tcgtagagat
     7321 ccttctcgtc gaccgtgctt gcggccttct cgatctggtc ggcgtcgtag aggatgacac
     7381 cgagatagcg aatgcgcccg acacaagtct ccgagcacac cgtcggctgg cccgcctcga
     7441 tccgcggata gcagagaatg catttctcgc tcttgccgct cgaccagttg aaatagatct
     7501 tcttatacgg gcagccggag acgcacatgc gccagccccg gcatttctcc tggtcgatga
     7561 gaacgatgcc gtcctcctcg cgcttgtaga tcgctcccga tgggcacgag gcgacgcagg
     7621 ccggattgag gcaatgctcg caaagccgcg gcaggtacat catgaaagtg ttttcgaact
     7681 ggccgtagat ctccttctgg acgccgtcga aattgacgtc cttcgatcgt ttggcgaatt
     7741 cgcctccgag gatttcctcc cagttcgggc cccactcgat tttctccatt cgctcgcccg
     7801 tgatgaggga gcgcggtcgc gccgtcggaa acgcctcgga ttccggcgcc ttctggagat
     7861 gctcgtaatc gaaggtgaac ggctcgtagt agtcgtcgat ctccggcatg ttcgg
//
LOCUS       DAORNH010000213         7913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6760_length_7913_cov_2.968058, whole genome shotgun sequence.
ACCESSION   DAORNH010000213 DAORNH010000000
VERSION     DAORNH010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6760_length_7913_cov_2.968058"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>786)
                     /locus_tag="PK264_18240"
     CDS             complement(<1..>786)
                     /locus_tag="PK264_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77848.1"
                     /translation="AQSRYDDDDDDRPPTYRDDSYRPPVRADAPDGDVADIRMFYEEL
                     APHGRWFEHRQYGYVWSPNVDEDWRPYSRGRWAHTEEHGWMWVSEEPFGWAVFHYGRW
                     GFDEDNGWFWVPGTDWGPAWVAWRHSDEHVGWAPLPPEATWEAGRGLSFSDSYLEDRR
                     YHPAWVFVPAASLTALAVYRYFSPRGRTTFFMRNTRPVTSYRFVNRRPYNVGWDVRRY
                     SSLIGRPVPPMRIFSAPNPRGSGFRDGSISIYRPRFTASPPGP"
     assembly_gap    787..808
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1230..3827
                     /gene="clpB"
                     /locus_tag="PK264_18245"
     CDS             1230..3827
                     /gene="clpB"
                     /locus_tag="PK264_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HRD77849.1"
                     /translation="MNFEIYTDRAKGFVQAAQNLATREGHQQITPLHLLKVLLDDPEG
                     LAASLVQRAGGNAEQAKAQTERALAKRPTVSGSGAGQTFATQELARLFAAAEKAAQQA
                     GDRFVSVERLLLALAVDAEAGALLKFAGVTPEKLAAAIADLRKGRTADSAGAEQGYDA
                     LKRYARDLTQAARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAIRIVNGDVPDSLRDRKLLSLDMGALIAGAKYRGEFEERLKSVLSEISSEGGKIILF
                     IDELHTIVGAGKADGAMDASNLLKPALARGELHCVGATTLDEYRKHIEKDAALARRFQ
                     PLFVTEPSVEDTVSILRGLKEKYELHHGVRITDSAIVAAAMLSHRYITDRFLPDKAID
                     LVDEASSRLKMEIDSKPEELDALDRDLVQRKIEREALRKETDAASRERLGRIETEIAD
                     LEEKSRALTLRWEGEKQKLGSATKIKEEIEQLRSELERAERRGDLARAAEIKHGRIPE
                     LEKKLSTVEGAAAGVLVEEAVTPDHIAGVVSRWTGVPVDRMLEGEKAKLLRMEDELAK
                     RVVGQREAIEAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAGFLFDDD
                     NALQRIDMSEYMEKHSVARLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHP
                     DVFNVLLQVLDDGRLTDGQGRTVDFKNTLIIMTSNVGAEHLAADAGRSDMEKAKELVF
                     AELKSRFRPEFLNRIDETIVFHRLERAQMARIVDIQMARLQKLLTDRKITLVLDDQAR
                     TWIANQGYDPAYGARPLKRVIQRNVQDPLAERLLAGEIKDGETVTISIRDGAITLNGW
                     PAKGRVAAA"
     gene            complement(3888..4775)
                     /locus_tag="PK264_18250"
     CDS             complement(3888..4775)
                     /locus_tag="PK264_18250"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD77850.1"
                     /translation="MALEIKQLHRDFVAEIGSVDLSKPISSEALAELQQAIDRYAVLV
                     FHGPRLSEEDQAEFALRFGELESQNGVLTTDVKRRISPKLVDISNLDEDNKVLGREDR
                     RRMFALGNQLWHTDSSFKRVPAKYSLLHAHSVTPEGGETQLVDMRAAYDRLSPKMKAR
                     VDGRIVEHSIFTSRAKLGFTEFSAEERRTLPPVHRDLVRVHPGSGRNSLYLASHASHI
                     VGEPLPDGRMLIADLMEQATLPEHVYTHRWAVGDLVMWDNRCTMHRGRPYDEAANRRD
                     MRRATTLDRDPAAADKRVA"
     gene            complement(4861..6336)
                     /gene="phnY"
                     /locus_tag="PK264_18255"
     CDS             complement(4861..6336)
                     /gene="phnY"
                     /locus_tag="PK264_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HRD77851.1"
                     /translation="MPATKIKTPKLPTKPHEKMRIAGKLVDAEARVEVRNPYTGDVVG
                     TVPAASPAQVAEAFKIAANYRSKLTRFDRQKILLRTAEILAKRRDEIATLITAESGLC
                     WKDSTYEVGRAYDVYSLAGQLAIRDDGEVYSCDITPHGKARRIYTMREPVQGAISAIT
                     PFNHPLNMISHKIAPAIATNNRVVAKPTELTPLTALLLTDILYEAGLPPEMLSIVTGN
                     PRTMGDAMIIDPNADVVTFTGSVKVGKYIAEKAGYKRIVLELGGNDPLIVMDDLSESD
                     LDKAAELAVQGATKNSGQRCTAVKRILVMEKVADKFVERVLAKAKKLKSGDPMDPETD
                     IGTVIHEGAAKTFEARVVDAVAKGAKLLHGNDRRGALYPATVVDHIPHTCELVREETF
                     GPVIPVIRCPNDLDEVIRISNSTAYGLSSGICTNRIDRVTKCVNELRVGTVNVWEVPG
                     FRIEMSPFGGIKDSGLGYKEGVAEAMKSYTNVKTYSLPWPG"
     gene            complement(6411..7634)
                     /locus_tag="PK264_18260"
     CDS             complement(6411..7634)
                     /locus_tag="PK264_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HRD77852.1"
                     /translation="MSDRNETKSPRKPVTDWATDLDHMDPRWTADPYPIWSELRQSCP
                     IAHSERYGGVHLPTRYDDVREIASDPARFSSKRPVVRANPPKVRLPSPPISSDPPHHG
                     PARQLLLPVFAPKAIDKLVPRTREICTELLDAIGDCETCDAAVNFAQHVPPRVIAMML
                     GVPEKDGDLFRHWIHVFLEETIADEEGGADRLVAVSREMDDYFRPFAEARRREPRDDL
                     ISHLVHAEMNGKPLTDNHFYGTLRLLLIAGIDTTWSAIGSSLLHLASVEQDRRRLAAE
                     PALIPTAVEEFLRAYAPVTMARQVVADTSVSGCPMKAGEMILLAFPAANRDPALFPNP
                     DEVIIDRKENRHATFGLGIHRCLGSNLARMELRTAIELWMARFPEFSLVPGGTVEWSK
                     GAVRGPRTVPVRIHR"
     gene            complement(7644..7883)
                     /locus_tag="PK264_18265"
     CDS             complement(7644..7883)
                     /locus_tag="PK264_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRD77853.1"
                     /translation="MSTTPAVRCLIVDLAKCQGHNRCYELAPELIDVDDLGKPVVRGD
                     GVVPSALEDKARLAVKNCPEYALRLVEPGSPGQEG"
BASE COUNT         1331 a         2517 c         2738 g         1305 t
ORIGIN      
        1 cgggtcccgg aggcgatgcc gtgaagcgcg ggcggtagat cgagatcgag ccgtcgcgga
       61 agcccgagcc gcgcgggttc ggggcggaaa agatccgcat cggcggcacc ggccggccga
      121 taagcgacga atagcgacgg acgtcccagc cgacgttgta cggccgccgg ttgacgaagc
      181 gatagctcgt caccgggcgc gtgttgcgca tgaagaacgt ggtgcggccc cgcggcgaga
      241 aatagcggta gactgcgagc gccgtgagcg aggcagccgg cacgaacacc caggccggat
      301 ggtagcgccg gtcctcgaga tagctgtcgg agaagctgag gccacggccc gcctcccagg
      361 tggcctcggg cggcaacggc gcccagccga catgctcgtc gctatgtcgc caggcaaccc
      421 acgccggacc ccaatcggtg cccggcaccc agaaccagcc gttgtcctcg tcaaagcccc
      481 agcggccgta gtggaacacg gcccagccga acggctcctc cgagacccac atccagccat
      541 gctcttccgt atgcgcccag cggcctcgcg agtaagggcg ccagtcctca tcgacgttcg
      601 gcgaccaaac atagccgtat tgccgatgct cgaaccagcg accgtgcggc gccagctcct
      661 cgtagaacat gcggatatcg gcgacgtcgc catccggcgc atcggcgcgc accggcggac
      721 gataggaatc gtcacgatac gtcggcggac gatcatcgtc gtcatcgtcg tagcgggact
      781 gtgcgcnnnn nnnnnnnnnn nnnnnnnnag cgtggagagc gccatggcga ggcctgcggc
      841 gagcgcagtc aggtggggag tgctgaggcg gtagcgcatc gcttggggtc ctcggcggtg
      901 gaaagtcggc agttccatct caggcgacca atgaccgcaa atccggctga acttgggccg
      961 gggacggcgc attgccggta cattcctgtt acctggcgca tgaatgccgg ctgaaccttt
     1021 gcatgcgtca agtcagacgt ctcaggcaca acagttagcg cattgcgacg aaaatgctcg
     1081 ccggcaccgc gcctgcgatg gcgccgcccc ccttttcgca aggtgtggca cgacctacat
     1141 gtccggtggg tgtgccgaca acgggcgccc agtgcagcga gatcacgggt gcgcaccgct
     1201 tcggggtggc gaaaccgggg agacacgaca tgaacttcga gatctatacg gatcgcgcca
     1261 agggcttcgt ccaggccgcg cagaacctcg cgacgcgcga gggccatcag cagatcaccc
     1321 cgctgcatct cctcaaggtg ctgctcgacg acccggaagg gctcgccgcc agcctcgtcc
     1381 agcgcgccgg cggcaacgcc gagcaggcga aggctcagac cgagcgcgca cttgcgaagc
     1441 ggccgacggt ctcggggagc ggcgctggcc agacgtttgc gacgcaggag cttgctcgcc
     1501 tcttcgccgc ggcggagaag gcggcgcagc aggcgggaga ccgcttcgtc agcgtagagc
     1561 ggctgctgct tgcgctcgcc gtggacgccg aggccggcgc actcctgaag ttcgcgggcg
     1621 tcacgccaga aaagctcgcg gccgcgatcg ctgacctgcg caagggccgc actgccgatt
     1681 ccgcgggtgc cgagcagggc tacgatgccc tgaagcgcta tgcccgcgac ctcacccagg
     1741 cggcgcgcga cggcaagctc gaccccgtca tcggccgcga cgaggagatc cgccgcacca
     1801 tccaggtgct ttcgcgccgc acgaagaaca atcccgtcct catcggcgag ccgggcgtcg
     1861 gcaagaccgc gatcgcggaa ggtctcgcca tccgcattgt caacggcgat gtgcccgaca
     1921 gcctgcgcga caggaagctc ctgtcactcg acatgggcgc gctgatcgcc ggtgccaagt
     1981 atcgcggcga gttcgaggag cggctcaagt ccgtcctctc ggagatctcc tccgagggcg
     2041 gaaaaatcat cctgttcatc gatgagctgc acacgatcgt cggcgccggc aaggccgatg
     2101 gcgccatgga cgcttcgaac ctcctgaagc ctgcactcgc acgcggtgag ctgcactgcg
     2161 tcggcgctac gacgctcgac gagtaccgta agcacatcga gaaggatgca gccctcgccc
     2221 ggcgcttcca gccgctgttc gtcaccgagc cctcagtcga ggacacggtc tcgatcctgc
     2281 gcggcctcaa ggagaagtac gagcttcacc acggcgttcg catcaccgac agcgccatcg
     2341 tcgcggcggc catgctctcc catcgataca tcacggaccg cttcctcccc gacaaggcga
     2401 tcgacctcgt cgacgaggcc tcgtcgcgcc tgaagatgga gatcgacagc aagcccgagg
     2461 agctcgatgc cctcgatcgc gacctcgtgc agcggaagat cgagcgcgag gcgcttcgca
     2521 aggagaccga cgccgcctcg cgcgagcgcc tcggtcgcat tgagacggag atcgccgacc
     2581 tcgaggagaa gagccgcgca ctgacgctgc gctgggaagg cgagaagcag aagctcggtt
     2641 ccgccaccaa gatcaaggag gagatcgagc agctccggag tgagctcgag cgtgccgagc
     2701 ggcgcggtga cctcgcgcgg gcggccgaga tcaagcatgg ccgtatcccc gaactcgaga
     2761 agaagctctc gaccgtcgag ggggccgctg ccggcgtgct cgtcgaggag gccgtgacgc
     2821 ccgatcacat cgccggcgtc gtctcgcgct ggacgggcgt tcccgtcgac cgcatgctgg
     2881 aaggcgaaaa ggcgaagctt ctgcgcatgg aggacgagct cgccaagcgc gtcgtcggcc
     2941 agcgtgaggc gatcgaggcc gtctcgacag cggtccgtcg cgcgcgggcc ggcctgcagg
     3001 atccgaaccg gccgatcggt tcgttcatgt tcctcgggcc gactggcgtc ggcaagacgg
     3061 agctcaccaa ggcgctcgcc ggcttcctgt tcgacgacga caacgcgctc cagcgcatcg
     3121 acatgtccga gtacatggag aagcactcgg tcgcgcggct gatcggcgcg ccgccaggat
     3181 acgtcggcta cgatgaaggt ggcgcgctca ccgaggccgt gcgtcgccgg ccctaccagg
     3241 tcgtcctgtt cgacgagatc gagaaggcgc atccggacgt gttcaacgtg ctgctccagg
     3301 tgctggacga cggccgcctc acggacggcc aggggcgcac tgtcgacttc aagaacacgc
     3361 tcatcatcat gacgtccaac gtgggtgccg agcaccttgc ggcggatgcc ggtcgcagcg
     3421 acatggagaa ggcgaaagag ctggtcttcg ccgagctcaa gtcgcgcttc aggcccgagt
     3481 tcctgaatcg gatcgacgag acgatcgtct tccaccgtct agagcgcgcg cagatggcgc
     3541 ggatcgtcga catccagatg gcgcggctcc agaagctgct caccgaccgc aagatcacgc
     3601 tcgtgctcga cgaccaggca cggacgtgga ttgcgaacca gggctacgac ccggcttatg
     3661 gtgcgcggcc actgaaacgc gtgatccagc gcaacgtgca ggacccgctc gccgagcggc
     3721 tgctcgcggg cgagatcaag gacggcgaga ccgtgacgat ctcgatccgc gacggtgcta
     3781 tcacgctgaa cggctggccg gcgaagggcc gcgttgcggc ggcctgattc ggtacgtcgg
     3841 ccgggtgcgg acgcaagttt ccccgcccgg acgatcgccc gcgaggctca ggcgacgcgc
     3901 ttgtcggcgg cggccggatc gcggtcgagc gtggtcgcgc ggcgcatgtc acgccggttg
     3961 gccgcctcgt cgtaaggccg gccgcgatgc atcgtgcagc ggttgtccca catgacgaga
     4021 tcgccgacgg cccagcggtg cgtgtagacg tgctcgggca gcgtcgcctg ctccatgagg
     4081 tcggcgatca gcatgcgccc gtccggcagc ggctcgccga cgatgtggga ggcgtgcgag
     4141 gcgaggtaga gcgaattgcg gccggagccc gggtgcacgc ggacgagatc gcggtggact
     4201 ggcggcagcg tgcgccgctc ctccgctgag aactcggtga agccgagctt cgcccgcgac
     4261 gtgaagatcg agtgctcgac gatgcgcccg tcgacccgcg ccttcatctt cggcgagagg
     4321 cgatcgtagg ccgcacgcat gtcgacgagt tgcgtctcgc cgccctcggg cgttacggaa
     4381 tgcgcgtgca gcagcgaata cttcgccggc acgcgcttga atgagctgtc agtgtgccag
     4441 agctggttgc cgagcgcgaa catgcggcgg cgatcctccc ggccgagcac cttgttgtcc
     4501 tcgtcgaggt tcgagatgtc gacgagcttc ggcgagatgc gccgcttgac gtcggtcgtc
     4561 agcacgccgt tctggctttc gagctcgccg aagcggagcg cgaactcggc ctggtcctcc
     4621 tccgagaggc gcggcccatg gaacacgagc accgcgtagc ggtcgatggc ctgctgtagc
     4681 tcggccaacg cctcactcga gatcggtttc gatagatcga cgctgccgat ctcggcgacg
     4741 aagtcacggt gcagctgctt gatctcgagg gccatcgcca tctccgacgg tgagcttcgc
     4801 cgacgttcgc ccgagtggac accaacgcta tcgcaatgtc gattgcgttt gcggcatacc
     4861 tcagcccggc cagggcagcg agtaggtctt gacgttggtg tagcttttca tggcctcggc
     4921 gacgccttcc ttgtagccga ggcccgaatc cttgattccg ccgaacgggc tcatctcgat
     4981 gcggaagccc ggcacctccc agacattcac agttccgacg cgtagctcgt tcacgcactt
     5041 cgtgacgcgg tcgatgcgat ttgtgcagat gccggaggag agcccgtagg cagtagagtt
     5101 cgagatgcgg atgacctcgt cgaggtcgtt cgggcagcgg atcaccggaa tgaccggtcc
     5161 gaacgtctcc tcgcgcacga gctcgcaggt atgcgggatg tggtcgacga ccgtcgccgg
     5221 gtagagcgca ccgcggcggt cgttgccgtg gaggagcttc gcgcccttgg cgacggcatc
     5281 tacgacccgc gcctcgaagg tcttcgccgc gccctcgtgg atcactgtgc cgatgtcggt
     5341 ttcggggtcc atgggatcgc cgctcttgag ctttttcgcc ttggcgagca ccctctcgac
     5401 gaatttgtcg gcaacttttt ccatgacgag gatgcgcttc acggccgtgc agcgctggcc
     5461 tgagttcttg gtggccccct gcacggcaag ctcggccgcc ttgtcgagat cgctctccga
     5521 gaggtcgtcc atgacgatca gcggatcgtt gcctccgagt tcgaggacga tgcgcttgta
     5581 gccggccttc tcggcgatgt acttgccgac tttgacgctc cccgtgaagg tgacgacgtc
     5641 ggcgttcgga tcgatgatca tggcatcgcc cattgtgcgc ggattgccgg tcacgatcga
     5701 cagcatctcg ggcggcaagc cggcctcgta aagaatgtcg gtgagcagca gcgcggtgag
     5761 cggcgtcagt tcggtcggct tggcgacgac gcgattgttg gttgcgatcg ccggcgcgat
     5821 cttgtggctg atcatgttga gggggtggtt gaacggcgtg atcgccgaga tcgcgccttg
     5881 cacgggctcg cgcatcgtgt agatgcgccg tgctttgccg tggggcgtga tatcgcagga
     5941 gtatacctcg ccgtcgtcgc ggatcgcgag ctggccggca agcgagtaga cgtcgtaggc
     6001 tcggccgacc tcgtaggtcg aatccttcca gcagaggccc gattccgccg tgatcagcgt
     6061 cgcgatctcg tcgcgccgct tggcgaggat ctcggccgtc ctgaggagga tcttctggcg
     6121 atcgaagcgc gtgagcttcg agcggtagtt ggcggcgatc ttgaaagcct cggcgacctg
     6181 cgcggggctc gcggccggca ccgtgccgac gacgtctccg gtgtaggggt tgcgcacctc
     6241 gacgcgcgcc tcggcatcga cgagcttgcc ggcgatgcgc atcttctcgt gcggcttggt
     6301 cggcagcttc ggcgtcttga tcttggtcgc aggcatcggg gatctccgga caaggcgggt
     6361 cagatcgtgg agtcgaccag cgcgagctgg ctggtcgagt ggcggaatgc ttaccggtga
     6421 atgcgaaccg gcacagtgcg aggaccgcgg acggcgccct tcgaccactc gacagtgcca
     6481 ccaggcacga ggctgaactc ggggaagcgc gccatccaga gctcgatcgc ggtgcggagt
     6541 tccatgcggg cgagattcga gccgaggcag cggtgaatgc cgagaccgaa ggtggcgtgg
     6601 cggttctcct ttcggtcgat gatgacctcg tccggattcg ggaagagggc cggatcgcgg
     6661 ttcgccgcgg ggaaggcgag caggatcatc tcgccggcct tcatcggaca gccgctgacg
     6721 ctggtgtcgg caacgacctg gcgggccatg gtgacgggcg cataggcgcg caggaactcc
     6781 tcgaccgcgg tcgggatcag ggcgggctcg gcggcgaggc ggcggcgatc ctgctcgacg
     6841 ctcgccaagt gcaggaggct cgagccgatc gcgctccatg tcgtatcgat gccggcgatc
     6901 aggaggaggc gcagcgttcc gtagaagtgg ttgtcggtga gcggcttccc gttcatctcg
     6961 gcgtgcacaa ggtgcgagat gagatcatcg cgcggctcgc ggcgccgcgc ctcggcgaag
     7021 ggacggaagt agtcgtccat ctcgcgcgag acggcgacga ggcggtcggc accgccttcc
     7081 tcgtcggcga tcgtctcctc gaggaagacg tggatccaat ggcggaaaag gtcgccgtcc
     7141 ttttccggta cgccgagcat catggcgatg acgcgcggag ggacgtgctg agcaaaattg
     7201 acagccgcat cgcacgtctc gcagtcaccg atcgcgtcga gcagctcggt gcagatctcg
     7261 cgcgtccgcg gtacgagctt gtcgatcgcc ttcggcgcga acacgggaag cagcagctgg
     7321 cgcgcgggtc cgtgatgcgg cgggtcggag ctgatcggcg gcgaggggag ccgcaccttc
     7381 ggcgggtttg cgcgcacaac cggtcgcttc gaggagaagc gggcaggatc actcgcgatc
     7441 tcgcgcacgt cgtcatagcg ggtcggcagg tgcacgccgc cgtaccgctc cgagtgggcg
     7501 atcgggcacg attggcgaag ctcggaccag atcggataag ggtccgccgt ccagcgcggg
     7561 tccatgtgat cgaggtccgt cgcccaatcc gtgacgggct tgcgtggcga tttcgtctcg
     7621 ttgcgatcgc tcatggtgct gcctcagccc tcttggccag gggagcctgg ctcgaccagt
     7681 ctcaaagcgt attccgggca gttcttcact gcgaggcgcg ccttgtcctc cagcgccgac
     7741 ggcacgacgc cgtcgccgcg caccaccggc ttgccgaggt cgtcgacgtc gatgagctcg
     7801 ggcgcgagct cgtagcagcg gttgtggccc tggcatttgg cgaggtccac gatgaggcat
     7861 ctgacggcgg gtgtggtcga catgagcgga tcacccttga ttggccgaag gat
//
LOCUS       DAORNH010000214         7899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6776_length_7899_cov_2.725013, whole genome shotgun sequence.
ACCESSION   DAORNH010000214 DAORNH010000000
VERSION     DAORNH010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6776_length_7899_cov_2.725013"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PK264_18270"
     CDS             complement(<1..185)
                     /locus_tag="PK264_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77854.1"
                     /translation="MPVARGALSDAERTGTRATAREKAHIEALRRWCDGDLDGMLGIW
                     EQILVDHPHDVMAFRLH"
     gene            196..>420
                     /locus_tag="PK264_18275"
     CDS             196..>420
                     /locus_tag="PK264_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD77855.1"
                     /translation="MKAKFCVEGRRALYDFLESRKVDHWKCGKLVVATEEAEVERIEA
                     IFVQATANGAEATAIRFGAGASTPELRNGGT"
     assembly_gap    421..430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<431..694)
                     /locus_tag="PK264_18280"
     CDS             complement(<431..694)
                     /locus_tag="PK264_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77856.1"
                     /translation="MVREVIQTLRFVSSVRRGHDTAGRICEAFHLLIGSNRSLRTIER
                     SHGPMALSKADVSVKTDVFAGRLLLELHELATPAATCRSTGGKS"
     gene            678..1736
                     /locus_tag="PK264_18285"
     CDS             678..1736
                     /locus_tag="PK264_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD77857.1"
                     /translation="MTSRTMLAALVGAAAVTLAPISGAALAQDKKVKVQMAGAFPSST
                     AILGPAQKRTVDMMRLMSGGSIDVKLFEPGALVPAGQYFDAVSNGSLDMAWTVSGFFT
                     GKNIAFALFSTVPFGPEAGEYLAWMRFGGGEQLMQDMYKPHKIVILNCGMISPEASGW
                     FRKEIKTLDDLKGLKMRFFGLGANVMQKLGVATQLLQAGEIFQALQLGTIDATEFSMP
                     AMDITLGFHQVAKFYYFPGWHQMATFSHLFISEAKWAEFSEAQKEIVRRSCDAQMLNQ
                     FAEGEAAQFAAMKELQAKGVQIKFWGPEFIKAFQEKWNEVAKETAEKSADFKKVWEHF
                     QAFRANYKIWKDHGYLKN"
     gene            1826..>2028
                     /locus_tag="PK264_18290"
     CDS             1826..>2028
                     /locus_tag="PK264_18290"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77858.1"
                     /translation="MLKVLTKGAVAGAMALAMVAGTAEAQDKKLKIQVAGAFTSSTAI
                     LGPAQNRFVDLMKKLSGGSLDAK"
     assembly_gap    2029..2041
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2042..2884
                     /locus_tag="PK264_18295"
     CDS             <2042..2884
                     /locus_tag="PK264_18295"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD77859.1"
                     /translation="GALVPAGQYFDAVSTGALDMAWSSVGFFTGREIAFALFSSVPFG
                     PESGEYLGWMREGGGEKLMQDITLNKFGFHSFICGIIPPEASGWFRKEIKTLDDLKGI
                     KMRFFGLGAIVMQKLGVSTQLIQPGEIFQALQLGTIDATEFSMPVMDLTLGFHQVAKH
                     YYFPGWHQMSSYQHLFVSKKKWDEMSETQREIVKASCDTNMLRMLAESEIVQVKAINE
                     IKAKGVTLHYWDKTFLDAMRKAWAEVVKEQSEKSAEFKKAWESLSAFREQYKVWKSYG
                     YLKE"
     gene            3010..>3395
                     /locus_tag="PK264_18300"
     CDS             3010..>3395
                     /locus_tag="PK264_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77860.1"
                     /translation="MSALLGLSRAIDAVLRFIAMTFGWSFLLLTLVIVFDVITRNGLK
                     IWLGFTTVQVLAPFQLSLFGIDLGSSRLQELQWHLHGILFLTWIGYAYIRNAHVRIDI
                     LTGGLSQRRQAWLELIGCILFALPYL"
     assembly_gap    3396..3405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3406..3677
                     /locus_tag="PK264_18305"
     CDS             <3406..3677
                     /locus_tag="PK264_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77861.1"
                     /translation="RGTFPGLVVAHLIVALPYAWQFFMTSFYQNEGSTAPNGLPARYI
                     IKGFLFFGFFSVLLAVISVAARRFVFLFGSKELSEAAMPAAKTGH"
     gene            3690..5069
                     /locus_tag="PK264_18310"
     CDS             3690..5069
                     /locus_tag="PK264_18310"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD77862.1"
                     /translation="MEWIGTHLAIIMFLVLTFIMFNGYPVAFMLGWVGVIFGFLGVYF
                     GTFNFLQFANLIPRVYGQAVENPVLVAVPMFIFMGTVLEKSGVAEELLRCLQVIFRRV
                     PGGLAIAVTLLGTIMAATTGIVGASVVMLTLIALPVMLERGYNKELSVGTIASAGTLG
                     ILIPPSIMLVIMGDLMQIPVGTLFAAAIIPGLIMSGIYLIYIMILCFFRPSLAPPLGA
                     HEGPQSRKELWTMIIKTFSPPAFLILLVLGSIFAGWATPTEAAGVGCAGAILLAIRAG
                     KWKMPIILDAIESSALTTGLVFFILFGATLFSYVFRALGGDDVVIEILHKVGIDTGWE
                     LLLFVMLLVFIMGFFFDWVEICLVVLPIFTPIMTKSVFPGFVDGQKVILLWFAILLAV
                     NMQSAFLTPPFGFALFYMKGTVPPSVNMGHIYRGIVPFVILQLLSLGVCMYWPETVLY
                     LPRRWGFLD"
     gene            5156..>5599
                     /locus_tag="PK264_18315"
     CDS             5156..>5599
                     /locus_tag="PK264_18315"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD77863.1"
                     /translation="MQGLLKLSNGIKRMLELIAFTSGWLLVVLMCVTCMDVISRKFGI
                     PIPLTKFQEFEWHLHTAIFSLWMGYNYTINAHPRVDSYTETLSYRTKAWIELVGCVLF
                     ALPYMCLMVYYGWSFTWLAWVQNEASENVTGLSHRWAIKLVFYIGL"
     assembly_gap    5600..5609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5733..7112
                     /locus_tag="PK264_18320"
     CDS             5733..7112
                     /locus_tag="PK264_18320"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD77864.1"
                     /translation="MVWLTENLSLVMFAVMFVIIFCGYPVAFVMGGTALVFAAIGWAI
                     GEFNPVKLSNILLRMWGGVAVDPVLVSVPMFIFMGSILERSGSAKDMLSATEILLKRV
                     PGGLAVAVMVMGTILAAPIGVVGAAVVLLSIISLPPMIAAGYDKRLAVGTVASAGTLG
                     ILIPPAIMLVVMAEMLNTNAGALFAAATMPGFLLSGLYLIYILAVAILKPAHAPRLPA
                     DFGPQTRAEFWRTMWRGLFPMTFLMTIVLGSIFTGWATATESAGVGVFGSFLIAALNG
                     RLNFAMANDAIRSACRANAMVFLIFLGATGFSYVFRELGGDDAMISLLGRMGIDSKWE
                     VLAFIMVLIFLLGFPFEWIEICLIVLPVFAPILLKFDFSDHLGHASYLMPWFATLVAV
                     NLQTSFMTPPFGATLFYLKGTAPKDITMSDIYQGMYPFVALQIVGLLLCVYYPSIALW
                     LPRVAGLLD"
     gene            7176..7511
                     /gene="ssrA"
                     /locus_tag="PK264_18325"
     tmRNA           7176..7511
                     /gene="ssrA"
                     /locus_tag="PK264_18325"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            7596..>7899
                     /locus_tag="PK264_18330"
     CDS             7596..>7899
                     /locus_tag="PK264_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HRD77865.1"
                     /translation="MSPGKPHINYDALQQDAFRGIVRAALVQVAESGLPGEHHFYISF
                     DTRAPGVILSKRLMELYQDEMIIVLQHRFWDLEVTPELFEVKLTFDGIPERLVIPFK"
BASE COUNT         1317 a         2516 c         2424 g         1599 t
ORIGIN      
        1 tgatggaggc ggaaggccat cacgtcatgg ggatgatcga ctaggatctg ctcccatatc
       61 ccgagcatgc cgtcgaggtc gccgtcgcac cagcgccgga gagcctctat gtgcgccttc
      121 tcgcgcgcgg ttgcgcgcgt gccagtcctc tcggcatctg acagagcgcc gcgcgcgacc
      181 ggcacgaccg gctccttgaa ggcgaagttc tgcgtcgagg ggcgtcgcgc cctctatgac
      241 ttcctggaaa gccgcaaggt cgatcattgg aaatgcggca agctggtcgt ggccacggag
      301 gaggcagagg tcgaacggat cgaggcgatc ttcgtgcagg cgaccgccaa tggcgcggaa
      361 gcgaccgcga tccgcttcgg cgccggagcc tcgacgcccg agttacgcaa tggcggtacc
      421 nnnnnnnnnn gctttttccc ccggttgatc gacaagtggc cgcaggtgtc gcgagctcgt
      481 gcagctccaa gagcaacctg ccggcgaaaa catcagtttt cactgataca tcagcttttg
      541 acaatgccat cgggccgtgc gagcgttcga tcgtgcgcag cgatcggttg gacccgatca
      601 gaaggtggaa agcctcgcaa attcgtcccg ccgtatcatg gccgcggcgc acactggaaa
      661 cgaaccggag ggtttgaatg acctcgcgca ccatgctcgc ggcgctcgtt ggcgccgctg
      721 ccgtcacact tgcgccgatc agcggcgctg cactcgccca ggacaagaaa gtcaaggtcc
      781 agatggcggg cgcattccca agctccaccg ccatcctcgg tcctgcgcag aagcgcaccg
      841 tcgacatgat gcgcctgatg tcaggcggat cgatcgacgt gaagctcttc gagccgggcg
      901 cactcgtgcc ggccggccag tatttcgatg cagtctccaa cggctcgctc gacatggcct
      961 ggacggtctc gggcttcttc acgggcaaga acatcgcctt cgctctgttc tcgaccgtgc
     1021 cgttcggtcc cgaggccggc gaatacctcg cctggatgcg cttcggcggc ggcgagcagc
     1081 tcatgcagga catgtacaag ccgcacaaga tcgtcatcct gaactgcggc atgatctcgc
     1141 ccgaggcctc aggctggttc cgcaaggaga tcaagacgct cgatgacctc aagggtctga
     1201 agatgcgctt cttcgggctc ggcgcgaacg tcatgcagaa gctcggcgtc gcgacgcagc
     1261 tcctccaggc gggcgaaatc ttccaggccc tccagctcgg cacgatcgac gcgaccgagt
     1321 tctcgatgcc ggcgatggac atcacgctcg gcttccacca ggtggcgaag ttctactact
     1381 tccccggctg gcatcagatg gcgaccttct cgcacctctt catctccgag gcgaagtggg
     1441 ccgagttctc cgaggcgcag aaggagatcg tgcgccgctc gtgcgacgcg cagatgctca
     1501 accagttcgc cgagggtgag gccgcgcagt tcgccgccat gaaagagctg caggcgaagg
     1561 gtgtgcagat caagttctgg gggcccgagt tcatcaaggc cttccaggag aagtggaacg
     1621 aggtcgccaa ggaaaccgcc gagaagagcg ccgacttcaa gaaggtgtgg gagcacttcc
     1681 aggcgttccg cgcgaactac aagatctgga aggaccacgg gtatctgaag aactgagctc
     1741 gcctcgatgc ccggatcgcg caggcgcggc ccgggcatcg ttgcgcatga gcgcaggcct
     1801 acccacaggg agcaacggag gaaacatgct caaggtactc acaaagggcg cggtcgccgg
     1861 tgccatggcg ctggccatgg tcgcaggcac tgccgaggcg caggacaaga agctcaagat
     1921 ccaggtcgcc ggcgccttca cgagttcgac ggccatcctc ggcccagcgc agaatcgctt
     1981 cgtcgatctc atgaagaaac tctcgggcgg ctcgctcgat gccaagtann nnnnnnnnnn
     2041 nggcgcgctc gtgcccgccg gccagtactt cgacgcggtc tcgacgggcg ccctcgacat
     2101 ggcgtggtcg agcgtcggct tcttcacggg tcgcgagatc gccttcgcgc tgttctcgtc
     2161 ggtgccgttc ggccccgagt cgggggagta cctcggctgg atgcgcgagg gcggcggcga
     2221 gaagctgatg caggacatca cgctcaacaa gttcggcttc cactccttca tctgcggcat
     2281 catcccgccg gaggcctcgg gctggttccg caaggagatc aagacgctcg atgacctcaa
     2341 gggcatcaag atgcgcttct tcggtctcgg tgccatcgtc atgcagaagc tcggcgtctc
     2401 cacccagctg atccagccgg gcgagatctt ccaggcgctc cagctcggca ccatcgatgc
     2461 gaccgagttc tcgatgccgg tcatggatct gacgctcggc ttccatcagg tcgccaagca
     2521 ctactacttc cccggctggc accagatgtc gtcctaccag cacctcttcg tctccaagaa
     2581 gaagtgggac gagatgtcgg agacgcagcg cgagatcgtc aaggcctcgt gcgataccaa
     2641 catgctgcgc atgctcgccg aatccgagat cgtccaggtg aaggcgatca acgagatcaa
     2701 ggcgaaaggc gtgacgctcc actactggga caagaccttc ctcgacgcca tgcgcaaggc
     2761 ctgggccgag gtcgtgaagg agcagtccga gaagagcgcg gagttcaaga aggcctggga
     2821 aagcctctcg gccttccgcg agcagtacaa ggtctggaag tcttacggct acctgaagga
     2881 gtgatgagct gaggggcgcg gatcaccgat ccgcgcccgc ttatgttcgg cggttcgatc
     2941 tgggggcgga tcgggcgggc cttggggaaa attctcggca cctgtgcgcg cacgacggag
     3001 gggatccgct tgtctgcctt attgggattg agccgcgcga tcgacgcggt cctacgattc
     3061 atcgccatga ccttcggctg gagcttcctg ctgctcacgc tcgtcatcgt gttcgacgtg
     3121 atcacgcgga acggcttgaa gatttggctc ggattcacga cagtgcaagt cctggcaccg
     3181 ttccagctca gcctgttcgg gatcgacctc ggatcgtcgc ggctgcagga gctgcagtgg
     3241 catcttcacg gcatcctgtt cctgacttgg atcggatatg cctacatccg caacgcgcat
     3301 gtgaggatcg atatcctgac gggcggcctc agccagcgca ggcaggcctg gctcgagctc
     3361 atcgggtgca tcctgttcgc actcccctat ctcatnnnnn nnnnntgcgc ggcacattcc
     3421 cgggccttgt cgtggcgcat ctcatcgtcg cgctgcccta tgcctggcag ttcttcatga
     3481 cgtccttcta ccagaacgag ggttcgaccg cgccgaacgg cctgcccgcg cgttatatca
     3541 tcaagggctt cctgttcttc gggttcttct cggtgctgct ggcggtcatc tcggtcgccg
     3601 cacggcgctt tgtattccta ttcggctcga aagagctctc tgaggccgcc atgccggccg
     3661 ccaaaaccgg ccattgaggg gaggcggtca tggaatggat tggaactcat ctcgccatca
     3721 tcatgttcct ggtgctgact ttcatcatgt tcaacgggta tcccgttgcc ttcatgctgg
     3781 gctgggtcgg cgtcatcttc ggcttcctcg gcgtctactt cggcaccttc aactttctgc
     3841 aattcgcgaa cctgatcccg cgtgtctacg ggcaagccgt tgaaaacccg gtactcgttg
     3901 ccgtaccgat gttcatcttc atgggcacgg tgctcgagaa gagcggcgtt gcagaagagc
     3961 tgctgcgctg tctgcaggtg atcttccggc gcgtcccggg cggtctcgcc attgccgtca
     4021 cgctgctcgg cacgatcatg gccgcgacaa ccggcatcgt cggcgcctcg gtcgtgatgc
     4081 tgaccttgat cgcgctcccc gtgatgctcg agcgcggcta caacaaggaa ctctccgtcg
     4141 gcaccatcgc ttcggcgggc acgctcggca tcctgatccc gccctcgatc atgctcgtga
     4201 tcatgggcga cctgatgcag attccggtcg gcacgctgtt cgccgctgcg atcatccccg
     4261 gcctcatcat gtcgggcatc tatctcatct acataatgat cctctgcttc tttaggccct
     4321 cgctcgcgcc accgctcggc gcgcatgagg gcccgcaatc gcggaaggag ctctggacca
     4381 tgatcatcaa gaccttctcg ccgccggcct ttttgatcct cctcgtcctc ggctcgatct
     4441 tcgcgggctg ggcgacgccg acggaggccg cgggtgtcgg ctgcgccggc gcgatcctgc
     4501 tcgccatccg ggccggcaaa tggaagatgc cgatcatcct cgatgccatc gaaagctcgg
     4561 cgctgacgac gggcctcgtc ttcttcatcc tgttcggtgc gacgctcttc tcctacgtgt
     4621 tccgggcact cggcggcgac gacgtcgtga tcgagatcct gcacaaggtc ggcatcgaca
     4681 ccggctggga gctcctcctg ttcgtgatgc tgctcgtgtt catcatgggc ttcttcttcg
     4741 actgggtgga gatctgcctc gtcgtgctgc cgatcttcac gccgatcatg acgaagtcgg
     4801 tcttccccgg cttcgtcgac gggcagaagg tgatcctgct gtggttcgcg atcctgctcg
     4861 cggtcaacat gcagtcggcg ttcctgacgc cgccgttcgg cttcgcgctc ttctacatga
     4921 aggggaccgt gccgccgtcg gtgaatatgg gccacatcta ccgtggcatc gtcccgttcg
     4981 tgatcctcca gctgctctcg ctcggcgtgt gcatgtactg gccggagacg gtgctctacc
     5041 tgccgagacg atggggcttc ctcgattagc gagaccatcg cgagcttgct gaaacgggcg
     5101 cctcgcggcg cccgttttcc tgagagcatg acaggatacc gatgggagac ggggcatgca
     5161 gggactgttg aaactgagca acggcatcaa gcggatgctc gagctgatcg cgttcacgtc
     5221 gggctggctg ctggtcgtgc tgatgtgcgt gacgtgcatg gacgtcatat cccgcaagtt
     5281 cggcattccc atcccgctga cgaaattcca ggagttcgaa tggcacctgc acacggccat
     5341 tttctccctc tggatgggct acaactacac gatcaacgcg catccacgcg tcgacagcta
     5401 caccgagacg ctgagctatc ggacgaaggc ttggatcgag ctcgtcggct gtgtgctgtt
     5461 cgcgctgccc tacatgtgcc tcatggtcta ctacggctgg agcttcactt ggctcgcctg
     5521 ggtgcagaac gaggcttcgg agaacgtgac cggcctatcg caccgctggg ccatcaagct
     5581 cgtcttctac atcggcctcn nnnnnnnnnt ggctcgtgct gctcgggatc gtgtcggtgc
     5641 tgatccggct cgtcgccttc ctgttcgcgg ggcgcacggc ctacgaagcc ggcctgcaga
     5701 tcggccactc cgagctggag gcgtgaggcg tcatggtgtg gctcactgag aacctctcgc
     5761 tcgtcatgtt cgccgtgatg ttcgtcatca tcttctgcgg ctatccggtc gcattcgtga
     5821 tgggtggtac ggcgctcgtc ttcgcggcca tcggatgggc gatcggcgaa ttcaatccgg
     5881 tgaagctctc caacatcctg ctcaggatgt ggggcggggt cgcggtcgat cccgtgctcg
     5941 tctcggtgcc aatgttcata ttcatgggct cgatcctgga gcggtccggc tcggcgaagg
     6001 acatgctgtc ggccaccgag atcctgctga agcgcgttcc gggcggcctc gccgtcgcgg
     6061 tcatggtgat ggggacgatc ctcgctgcgc cgatcggcgt ggtcggcgcg gcggtggtgc
     6121 tgctgtcgat catctcgctg ccgcccatga tcgcggccgg ctatgacaag cgcctcgccg
     6181 tcggaacggt cgccagtgcc ggtacgctcg gcattctcat tccgccggcg atcatgctgg
     6241 tcgtcatggc ggagatgctg aacaccaacg ccggcgcgct cttcgcggcg gcaaccatgc
     6301 cgggttttct gctctcgggc ctctatctga tctacatcct cgctgtcgcg atcctgaagc
     6361 cggcgcatgc gccgcgcctg ccggccgatt tcggcccgca gacgcgggcg gagttctggc
     6421 gcaccatgtg gcgcggactc ttcccgatga ccttcctcat gacgatcgtg ctcggttcga
     6481 tcttcaccgg atgggcgacg gcgaccgaga gtgcgggcgt cggcgtgttc ggctcgttcc
     6541 tcatcgccgc cttgaacggc cggctcaact tcgcgatggc gaacgacgcg atccgctccg
     6601 cctgccgggc caacgccatg gtgttcctga tcttcctcgg cgcgaccggc ttctcctacg
     6661 tctttcgcga gctcggcggc gacgacgcga tgatcagtct cctcgggcgc atgggcatcg
     6721 acagcaagtg ggaggttctg gccttcatca tggtgctgat cttcctcctc ggcttcccct
     6781 tcgagtggat cgagatctgc ctcatcgtgc tccccgtgtt cgcgccgatc ctcttgaagt
     6841 tcgacttctc ggatcacctc ggccacgctt cgtacctgat gccgtggttc gcgacgctgg
     6901 tggcggtcaa cctgcagacg tccttcatga cgccgccgtt cggcgcgacg ctcttctacc
     6961 tcaagggcac cgcgccgaag gacatcacga tgtccgacat ctaccagggc atgtacccct
     7021 tcgtggccct gcagatcgtg ggcctgctgc tgtgtgtcta ctatccctcg atcgcactct
     7081 ggttgccccg cgtcgcaggc ctgctcgact gagcggggcg ggctctccgg tccgcgtcga
     7141 aaagcgccgg gaggacgccg gatcgctctg tccaagctct tcccatcccg gacgggacgc
     7201 actatattcc ttgggccgga ttcgtccggc tatggcgata aatggacgtc gtaataagcc
     7261 attcggaccc gggggcggta cccggcgcct ccaccatcat gggggcgaaa taggatcgac
     7321 gagggtgtaa aggacgcgct tttgcccggc atgataccgc cgttatcggg tcaccagaat
     7381 agttgccaac gacaactatg ctgaggctgc tctcgctgcg taacgcggcg cgaaaggcct
     7441 gaaccaaagc cctggcgtct agccacgtca ggcgcggtcc ggagggcacc ggtcaacaga
     7501 agccctccac tttcgtcatc gcagcattct catcttcgtg ccaatcttct tcgtgcccgg
     7561 ccggagaccg gcgcccagga gacgccttac cgcccatgag ccccggcaag ccgcacataa
     7621 actacgacgc gctgcagcag gacgcgtttc gcggcatcgt gcgcgcagcc ctcgtccagg
     7681 tcgccgagag cgggctccca ggcgagcacc atttctatat ttcgttcgac acccgggcgc
     7741 cgggcgtgat cctctcgaag cgcctgatgg aactctacca ggacgagatg atcatcgtcc
     7801 tccagcaccg cttctgggat ctcgaggtga cgcccgagct gttcgaggtg aagctcacct
     7861 tcgacggcat cccggagcgg ctggtaattc cgttcaaag
//
LOCUS       DAORNH010000215         7892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6783_length_7892_cov_2.343116, whole genome shotgun sequence.
ACCESSION   DAORNH010000215 DAORNH010000000
VERSION     DAORNH010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6783_length_7892_cov_2.343116"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>346)
                     /locus_tag="PK264_18335"
     CDS             complement(<1..>346)
                     /locus_tag="PK264_18335"
                     /inference="COORDINATES: protein motif:HMM:NF025152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HRD77866.1"
                     /translation="MQRNAERDGQPPRDGNQRELSQRDLEKMLRDLENMARQGDRDMA
                     QQMLNELRDMLERLQPGGSRQADGQGQELMEMMDEFGNIIGREQQLLDDTFKQQRRRG
                     ERGDRGDKGKRG"
     assembly_gap    347..356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<357..>1730)
                     /locus_tag="PK264_18340"
     CDS             complement(<357..>1730)
                     /locus_tag="PK264_18340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HRD77867.1"
                     /translation="PADSPSGRLWVAHRDRLQRLLDRLRVGGPKPRTDRYDPIGLRVL
                     LGLGLVVAFAFAGDSVSDRLRSAFRFGPSQAQIAEARLDAWVTPPAYTGRPPVMLADG
                     ARGGFRPDLKGEAIEVPERSQIVVRTAGTVLKALALEIEPDGGPVMRLEADASAPVSD
                     GRPAGVIELKSELKTSATVRVVTRGVQMVSWRFKVIPDTPPKIALTKNPESLPRGSLK
                     LSYKVEDDYGVITIDGRLRRVARKAGDPATAWARPKPKTGPRLPMMRPPHLVLRLPRD
                     NARSADGVSFHDLGAHPWAGTRVRLGLVAKDALEQTGRSEVVEFVLPARQFRKTLAKA
                     IVEQRRKLVEDSRNVPDVLEGIEAITFAAADFNMDRGVFLGLRAVYHRLQLDRNAKTP
                     HGTRQVLASAVEHLWHIALRAEDGNLSAAERRLRDAQDRLAEALKNGATDEELRQLMQ
                     ELRQALN"
     assembly_gap    1731..1744
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1745..2109)
                     /locus_tag="PK264_18345"
     CDS             complement(<1745..2109)
                     /locus_tag="PK264_18345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HRD77868.1"
                     /translation="MSERHTITSSTPSRVGAGDKQLLPPTLERKIARARWALFFERLW
                     PRFWLFLAVAGLFLLVSIAGLWLHLDETTHKIVLAGFVVAGLAALVPVARVAWPAREE
                     AIHRIERVSGVPHRPASSYA"
     gene            complement(2301..>2912)
                     /locus_tag="PK264_18350"
     CDS             complement(2301..>2912)
                     /locus_tag="PK264_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD77869.1"
                     /translation="AEERPWEWRPQLLALNPSGELPILEVDSGLVLCGAYAISEYIAE
                     EIDATSKDRRVTELFPGSRDERAEIRRLVDWFHRKMDREVTRDLLAERVYALMKPDAA
                     RPQPDVSVLKAARANLKYHLSYMSYLAETRRFIAGDEISFADLAGAAHLSTLDYLNEI
                     AWDTQPAVKDWYQRLKSRRSFQPLLADRVPGLAAPGHYANIDF"
     assembly_gap    2913..2933
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3017..3676)
                     /locus_tag="PK264_18355"
     CDS             complement(3017..3676)
                     /locus_tag="PK264_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD77870.1"
                     /translation="MAVSRDAIVDQLLASFAAGRRAEAPYPHWFLKDCVSSAALDEIL
                     ALPFPAPALGGVSGKREIHNATRRYFDQENMGRFPICRAFNEAFQSREVTGAVEHTFG
                     SRLAGTYLRVEFAQDTDGFWLEPHTDLGVKSFTMLLYASREPAHAGLGTDIYDAEKRH
                     LGRSPFTPGGAMVFVPSKVTYHGFEARPISGVRKSIIINYVTNEWRAREQLAYPDRPV
                     A"
     gene            complement(4149..4406)
                     /gene="moaD"
                     /locus_tag="PK264_18360"
     CDS             complement(4149..4406)
                     /gene="moaD"
                     /locus_tag="PK264_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HRD77871.1"
                     /translation="MQLKILYFAWVRERIGRAGETIELTARVRTVADLIAALKSRGPE
                     YAHAFERADIIRAAIDHVHVKHDAPITGAREVAFFPPVTGG"
     gene            complement(4410..4970)
                     /gene="pgsA"
                     /locus_tag="PK264_18365"
     CDS             complement(4410..4970)
                     /gene="pgsA"
                     /locus_tag="PK264_18365"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRD77872.1"
                     /translation="MLSLPNVLTYLRILAVPALAACFLLVKPPDGRWLAMGIFGLACI
                     TDWLDGYIARAWGLQSTLGRILDPIADKLLVGTALMLLVHDNTIDGVHIIAALVILCR
                     EILVSGLREFLAELNVRIHVTTLAKYKTSLQMLALGVLLLGPAGDRITAGVTATGIVL
                     LWLAALLTLWTGYDYLRAAIRSAIER"
     gene            complement(5122..5961)
                     /locus_tag="PK264_18370"
     CDS             complement(5122..5961)
                     /locus_tag="PK264_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77873.1"
                     /translation="MSAASRTLLLAMLLALAGCFDGRTTIDFSVPGKTITEVELNPAP
                     DLDPIIELSASMAALKGDERQPSGGALCAQFFGNWRGRAASREIVTVSAATIGGRLAC
                     TIRSEGESWGSETSFADVASQFSERREKLLREFADGPLAKAKGLVEALNAIDLRREGP
                     RRARFEIDLGRFIGEDRAVIPRLMMELMFGRMGHKTSQIPDAVLAKSFNRALVASLRM
                     LVRGHETRRLVYEVRLARIVETNMPRRGDTVVIGGTYAEWHAAFEAPDGPARAKYYLV
                     FEF"
     gene            complement(5982..6923)
                     /locus_tag="PK264_18375"
     CDS             complement(5982..6923)
                     /locus_tag="PK264_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77874.1"
                     /translation="MSTSYVRRRGQQLANLLVRAWAPVRALSITALLALPLTACLDGR
                     VLVDIKPGGLTEIEVEVRGAPEFASIVELVAAAGDLKSENNPLAGKSLCHAMAEGINQ
                     SNELKAKAPPLIATSVRLLEGARPGCVIRMAITEPLSDEVVKKLGDGLKTWNAQVADM
                     MPGLVSAGKGLTLVSDGPRRVRIVSEPGPALSLNETRDLFVPLIKRLVADEMMKGKPM
                     PGLPAGTLARIMDDANLAKLVESGMRANLAAYRIALKGYEDYRIDIELAVPRIIETNL
                     ERKGDRLSLGQSYGDFLKAMADPKTVPPRPFVLVFEY"
     gene            complement(7057..>7892)
                     /locus_tag="PK264_18380"
     CDS             complement(7057..>7892)
                     /locus_tag="PK264_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77875.1"
                     /translation="AAGRPAGARALRGALVATVAMLAATLGGCLEGRVLLDVRADGNA
                     GVVIDAKGAPEFKEVLDLIPATMGIAPSPMARAGAGKPGCEAIAAAMEADPATPPIGK
                     TIKAKQSMAGNRPACTFSMDIGKGDELVQAIEKGLAAAATMPGQKMEWLKVKSEGPRR
                     YRFEFDLERYFADERANMQETVKMLIGMAGAGVPADSVLAKIAQAYDRAMLGTTRVMA
                     KGYEAYRFDVAIRAPKIIETNMKRDGNTASFGYSFADWVKFSESVDSRKGQKVFVVFE
                     Y"
BASE COUNT         1339 a         2603 c         2582 g         1323 t
ORIGIN      
        1 tcgccgcgct tgcccttgtc gccacggtcg ccgcgttcgc cgcggcgacg ctgctgcttg
       61 aaagtatcgt cgagcagctg ctgctcgcgg ccgatgatgt tgccaaactc gtccatcatc
      121 tccatgagct cctggccctg cccgtcggcc tgccggctgc cgcccggctg gagccgctcg
      181 agcatatcac ggagctcatt cagcatctgc tgcgccatgt cgcgatcacc ctgccgcgcc
      241 atgttctcga ggtcgcggag catcttctcg aggtcgcgct gggaaagctc acgctggttg
      301 ccgtcgcgcg gaggctggcc gtcacgctcg gcgttgcgct gcatctnnnn nnnnnncgtt
      361 gagcgcctga cgcagctcct gcatgagttg gcgcagctcc tcgtcggtgg caccgttctt
      421 gagggcctcg gcgagccgat cctgcgcatc acgaagccgc cgctcggccg cggagagatt
      481 gccgtcctcg gctcgcagag caatgtgcca aagatgctcg acggcgctgg cgagcacctg
      541 tcgcgtgcca tgcggggtct tcgcgttccg gtcgagctgc aggcggtgat agacggcgcg
      601 cagcccgagg aacacgcctc ggtccatgtt gaagtctgcg gccgcgaagg tgatcgcctc
      661 gatgccctcc agcacgtcag ggacgttgcg cgaatcctcg acgagcttgc gccgctgctc
      721 gacgatcgcc ttggcaagtg tcttgcggaa ttggcgggcc ggcagcacga actcgacgac
      781 ctcgctgcgc cccgtctgct cgagcgcatc cttggcgacg agcccaaggc ggacgcgcgt
      841 tccggcccag gggtgagcgc ctagatcgtg gaagctcact ccgtcggccg agcgcgcgtt
      901 gtcgcgcggc agccgcaaga cgaggtgcgg cggccgcatc atcgggaggc gcggccctgt
      961 cttcggcttg gggcgtgccc aggccgtcgc cggatcgccg gccttgcgcg ccacacgccg
     1021 caggcgaccg tcgatcgtga tcacgccgta gtcgtcctcg accttgtagc tgagcttcag
     1081 cgagccgcgc ggcagcgatt cgggattctt cgtgagcgcg atctttggtg gcgtatcagg
     1141 gatcaccttg aagcgccagc tcaccatctg tacgccgcga gtgacgacgc gcactgtcgc
     1201 cgaggttttg agttcgctct tcagctcgat gacgccggcc gggcgaccat cgctgaccgg
     1261 cgcactggca tcggcctcga gccgcatgac cggcccgccg tcgggctcga tctcaagtgc
     1321 caacgccttg agaacggttc cagcggtacg cacgacgatc tggctacgct ccggaacctc
     1381 gatcgcctcg cccttgagat cggggcggaa gccgccgcgg gcgccgtcgg ccagcatgac
     1441 aggcggacgg cccgtatagg cgggcggcgt cacccaggca tcgaggcgcg cttccgcgat
     1501 ctgtgcctgc gacggtccga agcgaaaggc agacctcagt cgatccgaga cgctgtcgcc
     1561 ggcaaaggcg aacgcgacga cgagcccgag gcctaggagc acacgaaggc cgatcgggtc
     1621 atagcggtcc gtgcgcggct tcggcccgcc gacccggagc cgatccagaa gccgctgcag
     1681 gcgatcgcga tgtgcgaccc agagccgccc gctcgggctg tcggcgggcg nnnnnnnnnn
     1741 nnnngcatag gacgaggcag gccggtgggg gacacccgac acccgctcga tgcgatgaat
     1801 ggcctcttcg cgggcgggcc aggcaacgcg cgcgacaggg acgagggccg cgagcccggc
     1861 gacgacgaag cctgcgagca cgatcttgtg cgtcgtctcg tcgagatgca gccagaggcc
     1921 ggcgatcgag acgagcagga agaggccggc aacggcgagg aacagccaga agcggggcca
     1981 gagccgctcg aagaagagtg cccagcgcgc gcgggcgatc ttgcgctcga gcgtcggcgg
     2041 gagcagctgc ttgtcgcccg ctcccacgcg ggacggggtg gacgatgtga tcgtgtggcg
     2101 ctctgacatg ccgtcaccct agcatgggat tttcatcggc gtccccggtt gctcacgcaa
     2161 ttgtttccgg ctcgcgacct ttcaatcgga gccgcgccga ccggatggcg ccgcgctgcg
     2221 agtgtcgctc cgcctgtggc aagatggtgg caggaagatg aagttcccgt aatggccggc
     2281 aatgcacgcg gccggcccgt ctagaaatcg atgttggcgt agtgccccgg ggccgcgagg
     2341 cccggcaccc ggtcggccag cagcggctgg aacgagcgac ggctcttcaa gcgctgatac
     2401 cagtccttga ccgccggttg cgtatcccac gcaatttcgt tgagatagtc gagcgtcgaa
     2461 agatgcgcgg cacctgcaag gtccgcaaag ctgatctcgt cgcccgcaat gaaccgccgt
     2521 gtttcagcaa gataggacat atagctcaag tggtacttga ggttggcacg cgcagccttg
     2581 agcaccgaga cgtccggctg cggccgtgct gcgtcgggct tcatgagcgc atagacccgt
     2641 tcggcgagca gatcccgtgt cacctcgcga tccatcttgc ggtggaacca gtcgacaagg
     2701 cggcggatct cggcgcgctc gtcgcggctc cccggaaaca gctcggtaac gcgccggtcc
     2761 ttgctcgtcg cgtcgatctc ctcggcgatg tactcggaga tggcgtaggc gccgcacagg
     2821 acgaggccgc tgtcgacttc gaggatgggg agttccccgg atggattgag cgcgaggagc
     2881 tgcggccgcc actcccacgg ccgctcctcg gcnnnnnnnn nnnnnnnnnn nnncgagcgc
     2941 gaggcggatg ctgcgcgaca gcgggcagag gcggtactgc gtgagcttca gcatgagagc
     3001 cggaccagcg ccgggatcag gcaaccggcc ggtcgggata ggcgagctgc tcgcgagcgc
     3061 gccactcgtt ggtgacgtaa ttgatgatga tcgacttccg cacgccggag atcgggcggg
     3121 cctcgaagcc gtggtaggtg accttcgagg gcacgaacac catggcgccg ccgggcgtga
     3181 agggcgagcg gccgagatgg cgcttttcgg cgtcatagat gtcggtgccg aggcccgcgt
     3241 gcgcgggctc gcgcgaggca tagagcagca tcgtgaacga cttcacaccg aggtcggtgt
     3301 gcggctcgag ccagaagcca tcggtatcct gcgcaaattc cacccgcagg taggtgcccg
     3361 cgagcctgct gccaaaggtg tgctcgacgg cgcccgtcac ctcgcgcgac tggaacgcct
     3421 cgttgaaggc gcggcagatg gggaagcgcc ccatgttctc ctgatcgaag tagcgccgtg
     3481 tcgcattgtg gatctcacgt ttgcccgaga ccccgccgag cgccggcgcc ggaaacggaa
     3541 gcgccagaat ttcgtcgagc gctgcgctcg acacgcagtc tttcaggaac cagtgcgggt
     3601 agggtgcctc ggcacgccga ccggcagcga acgaggcgag gagctggtcg acgattgcgt
     3661 cacgggaaac ggccatggga tcaccggttg agagtgccga tagtcgccgg tcaccagagg
     3721 ggtgcgtcgg ggacgcggaa cctcgacttg ccggctcggg attaccggag ctagcattcg
     3781 acggctacca ctccaacgga cacggcgtcg cgcgcggcgg cccgccgcac gagaaagctc
     3841 attgcggtga ccggatgctg tgcctcggta agacggatgg cctcggtgag acggatgcgc
     3901 gctccggaaa agccacgagg ctccccgaat cggaccagcg ctccgaaaag caaacgtggc
     3961 cgagcgaggc ttcaggcagg tccgccgctc ccttggggag ctttatggcg cggaggccgt
     4021 gcgccttgag ctccaggagc tgagagataa tcccttcgga ccgtccgtcg gcctcgcatc
     4081 ggccaacgga catagttaac attatggctg tcgccacctg ttgccgatcg cagttgaacg
     4141 catccagctc agccacccgt caccggtggg aagaaggcga cttcacgcgc ccctgtgatt
     4201 ggcgcatcgt gcttcacatg cacatgatcg atcgcggcgc ggatgatgtc cgcgcgctcg
     4261 aaagcgtgcg catactccgg accgcgcgat ttgagggcgg cgatcagatc agccacggtc
     4321 cgcactctgg cggtcagctc gatcgtctcg ccagcacgcc cgatccgctc gcgcacccaa
     4381 gcgaagtaga gaattttgag ttgcatcacc tagcgctcga tggccgagcg gattgccgcc
     4441 cggagatagt cgtagccggt ccacagcgtc agcaacgccg cgagccagag gagcacgatc
     4501 cccgttgccg tgacgcccgc cgtgatccga tcgccagctg gcccgagcag gaggacgcca
     4561 agcgcgagca tctgcaggct cgtcttgtac ttggcaaggg tcgtcacgtg gatgcgcaca
     4621 ttgagctcgg ccaggaactc gcgcagtccc gagacgagga tctcccggca gaggatcacg
     4681 agcgccgcga tgatgtgcac gccgtcgatc gtgttgtcgt gcacgagcag catgagcgca
     4741 gtgcccacga gcagcttgtc ggcgatcgga tcgagaatgc gtccgagcgt cgattgcaga
     4801 ccccaggcgc gcgcgatgta accgtcgagc cagtcggtga tgcaggcaag tccgaagatg
     4861 cccatggcga gccagcgccc atcgggcggc ttcacgagaa gaaagcaggc cgcaagcgcc
     4921 ggcaccgcca aaatcctcag gtaggtgagg acattgggca ggctcagcat cagcggtctc
     4981 ggcgcggcac ggtccatgcg atagtggtac accatcgcga cccggtgccg tcaatggcgg
     5041 ctgccagtgg ctcttttggc tctgtgtact gttgcgcact tgcggcgagt gcgttgcgcc
     5101 gatgccacgt catggcgagc cttagaactc gaacaccagg tagtactttg cgcgcgccgg
     5161 accatccgga gcctcgaatg ccgcgtgcca ttcggcgtag gtccccccaa tgacgacggt
     5221 atcgccgcgg cgcggcatgt tggtctcgac gatcctcgca aggcgaacct cgtaaaccag
     5281 ccgccgcgtc tcatggcccc gcacgagcat gcgcagtgac gcgaccagcg cccggttaaa
     5341 gctcttggcc aggaccgcgt ccgggatttg cgacgtctta tggcccatcc gcccgaacat
     5401 gagttccatc atcagtcgag gaatcacggc gcgatcctcg ccgatgaagc ggcccagatc
     5461 gatctcgaaa cgggcgcgac gtggtccctc gcgacgaagg tcgatggcat tcagggcctc
     5521 gaccaaccct ttggccttgg ccaaaggtcc atcagcgaac tcacgcagca gtttttcacg
     5581 gcgctcggag aactggctcg cgacatcggc gaacgaggtc tcacttcccc agctctcgcc
     5641 ttcggatcgg atcgtacaag cgagcctgcc tccgatcgtc gctgcagaaa ccgtcacgat
     5701 ctcgcgcgag gctgccctcc ctcgccagtt accgaagaac tgcgcacaaa gagccccgcc
     5761 gctcggctgg cgctcgtccc ccttcagggc cgccatggac gcgctcaact cgatgatggg
     5821 atcgaggtcc ggcgccgggt tcagctcgac ttccgtaatc gtcttgccgg gcaccgagaa
     5881 atcgatcgtc gtgcgcccgt cgaagcatcc agctaacgcc agcagcatgg ccaagagcag
     5941 ggttctggat gcggcgctca tcgaatgctc cgacaggctc ctcaatactc gaacacgagg
     6001 acgaagggtc gtggcggcac cgtcttcgga tcggccatcg ccttcaggaa gtcgccgtag
     6061 ctctgcccga ggctcagccg atcccccttg cgctcgagat tggtctcgat gatgcgcggc
     6121 acggcgagct cgatgtcgat gcgatagtcc tcgtagccct tgagtgcgat ccgataggcc
     6181 gccaaattcg ctcgcatgcc cgactcgacg agtttggcga gattggcgtc gtccatgatc
     6241 cgcgcaagcg tgcccgccgg caggccgggc ataggcttgc ccttcatcat ctcgtcggca
     6301 acgagccgct tgatgagcgg tacgaataga tcgcgcgtct cgttgaggct cagcgctggg
     6361 cccggctccg agacgatccg cacgcgccgc gggccatcgc tgacgagcgt gagccccttc
     6421 cccgccgaga ccaggcccgg catcatatcg gccacctgcg cattccaagt cttcaagccg
     6481 tcgccgagct tcttgaccac ctcgtcgctc aacggctccg tgattgccat gcggatgacg
     6541 cagccgggac gcgcgccttc aagcaggcgc accgaggttg cgatcagcgg cggcgccttg
     6601 gccttcagct cgttgctctg gttgatccct tcggccatgg catggcagag cgacttgccc
     6661 gccagcggat tgttctcgga cttgagatca cccgccgccg cgaccagctc gacgatgctc
     6721 gcgaactccg gagcaccacg cacctccacc tcgatttcgg tgagcccgcc cggcttgatg
     6781 tcgacgagta cgcgcccatc gaggcacgcc gtgagcggca gggccagcag cgcggtaatc
     6841 gacagtgcgc gcaccggagc ccaggccctg accagcaggt tcgcgagttg ctgccctctc
     6901 cgcctcacat agctcgtgct catgattgac ctccgctcat acgggaacca ggaatgcgag
     6961 atccaggaaa gcgagaccta caagcaagta cgccgacgcc tcgcggcgcc ggcgcatcgg
     7021 ggtcgcgttg ccggcgccgc ttggcgccac attcgatcag tactcgaaca ccacgaacac
     7081 cttctgtccc ttgcggctgt ccacgctttc agagaacttc acccagtcgg cgaacgagta
     7141 tccgaacgag gcggtattgc cgtcgcgctt catgttggtc tcgatgatct tcggcgcgcg
     7201 aatcgcgacg tcgaagcgat acgcttcgta tcccttggcc atcacgcgtg tcgtcccgag
     7261 catcgcgcgg tcgtaggcct gggcgatctt ggcgagcaca ctgtccgccg gaacgccggc
     7321 acccgccatg ccgatcagca tcttgacggt ctcctgcatg ttggcacgct cgtcggcgaa
     7381 gtagcgctcg agatcgaact cgaagcggta acggcgcggc ccctcgctct tgaccttcag
     7441 ccactccatc ttctgtcccg gcatggtcgc tgcggccgcg aggcccttct cgatcgcctg
     7501 gacgagttcg tcacccttgc cgatgtccat cgagaacgta caggccggac ggttgcccgc
     7561 catgctctgc ttggccttta tggtcttgcc gatgggcggt gtagctggat cagcctccat
     7621 ggcggcggca atcgcctcgc agcccggctt gcccgctccc gcccgggcca tgggggaggg
     7681 cgcgatcccc atcgtggccg ggataaggtc cagcacctcc ttgaactcgg gcgcgccctt
     7741 ggcatcgatg acgacgccgg cattgccgtc cgcgcggaca tcgagcagaa cgcggccctc
     7801 gaggcagcct ccgagtgtcg ctgcgagcat ggccacggtc gcaacgagcg ccccgcgcag
     7861 agcgcgcgcg ccggccgggc gtcccgcggc gg
//
LOCUS       DAORNH010000216         7794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6909_length_7794_cov_2.849464, whole genome shotgun sequence.
ACCESSION   DAORNH010000216 DAORNH010000000
VERSION     DAORNH010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6909_length_7794_cov_2.849464"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PK264_18385"
     CDS             complement(<1..526)
                     /locus_tag="PK264_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77876.1"
                     /translation="MKALVLIPTTTRPVIITEIRERRSLPASSVVVEGDFRALPISRD
                     YDTLVGAAGPLGGLLGSPGLPSHQLKLSEAFETGRSWEVPVSIAHVLIERGVELVSAP
                     EDADLIVFATGAVDSDLGIVKERYFLGQKIPAASELLAKARTNARIVALLPDGATDGD
                     GGPEHARGVAGWLAA"
     gene            complement(617..1216)
                     /locus_tag="PK264_18390"
     CDS             complement(617..1216)
                     /locus_tag="PK264_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77877.1"
                     /translation="MTKPPDDRDQKPTPLQLKDDKSPAMLQHLVAELNARDLVTAMRD
                     TRAESTASATGIANSELIGYALRGPGAPMDFRIERAIRSDRTVGLRYRRMLQSSAIGY
                     SELAMAAGTGTYPSRRVGAFDLRVVEDGRDVFVVLSQSAGGPAGPQALEAIGPDDSVR
                     IPLSEAVRGHIQIAIDPANGDLSRLLKLLGEATTELYLI"
     gene            complement(1213..2667)
                     /locus_tag="PK264_18395"
     CDS             complement(1213..2667)
                     /locus_tag="PK264_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77878.1"
                     /translation="MDVAQIDQQTINELNGIVESRRLGAADQKKLANFVAGAIRPNRD
                     RPVFDLVRIIAEIGAKVVAGKPPGRTDVDRCLAVLLGPVGAVVSNCKGKLPTHEIGPL
                     MGFLEFLLMHSDCSYFAEMRRIIGELAHAPAAGREAAVKAAVSDIASRLRAYRTERFP
                     VARHEKVFRSLLGYLGTKPRRTSAIQFDDEDIVAFWDSQIREGNRLLYRTVVKHFLTL
                     ETVAADVSAQRAMNDAVSLYDSDDGRVLAPVEMATENVEPIGDPIELRYEALVEFLRT
                     GLPEGVKLLTGTERDRLADLAECGPFTVSRPLTALRYLSFGAVQSGIANFLRRGSGGS
                     DVAGRVDCGDATAYPDVEKGYVKLADHLSKLLLISAFLAAPAGNDNPALASVEPDALA
                     EARREGQGFLKRLKREGFDEAPDVLARSVVPVAGELVQIREVVQGFIGGVGNLQRGAE
                     LNSRFIDDKVRFAAAFRAAYLAPGGASTVPGASS"
     gene            2977..4023
                     /locus_tag="PK264_18400"
     CDS             2977..4023
                     /locus_tag="PK264_18400"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD77879.1"
                     /translation="MRRLASLAAFAAAMALSTPLSAQGIEKRSFNVIGTWGNLTNWQK
                     LEAPFWKEVLPKASGDALKINVRSLTEANLKGTELLRLLKQGVYDFAAVLPIYVDDGG
                     AVIEASDLAGVARDFAMARKIADLWLPEMQKTMGDKHGAIILAHFPFPQQVFFCRGDI
                     KSVADLKGKKIRVQGASQADFVTAVGGSPVTMAFGDVLPALEKGVVDCGITGTMPAYK
                     TKWHEVVGTLFQLPVGFTLGTWIVNAKTWNSLPKATQDLIKKEMDALTDRSWKTIEAE
                     SDEGVACNTGSGACSAGSPGKVKLVKPSDADIAARETALNTVVLANWAKRCTPDCVAK
                     WNEIVGKPYKLNAK"
     gene            4110..4799
                     /locus_tag="PK264_18405"
     CDS             4110..4799
                     /locus_tag="PK264_18405"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD77880.1"
                     /translation="MEALWRLIQHLARIGAWAGGGLLILAALTVSTEVLLRKGLGALV
                     GYPILITGTNEIAAYLFAIGTSWSLAYALVTRGHVRIDTLYERLSPHIRAILDIIALL
                     ALALFVAVLVERAYDLVADSYLSDRRSATPLRVKMAIPQIAWFAGIGRPNIMMVVAML
                     IAFLRSLLALAHGDPGTVSRTIGVPSQKEEIRNELEGLGIGRPAGATDPTPPNGPKAA
                     PTSRGKPADWT"
     gene            4803..>5093
                     /locus_tag="PK264_18410"
     CDS             4803..>5093
                     /locus_tag="PK264_18410"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD77881.1"
                     /translation="MLATALILLAVMLAISVPVAAVLGVLGLSLDQFFADARLRRAMS
                     DILWEQSANDILIAIPMFVMLGEVLLRSGIAERVYAAIAQWLSWLPGGLTHAN"
     assembly_gap    5094..5217
                     /estimated_length=124
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5218..6100
                     /locus_tag="PK264_18415"
     CDS             <5218..6100
                     /locus_tag="PK264_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD77882.1"
                     /translation="TLAAGGTLGILIPPSINFILYGLLVNQSVPRLYLAGFIPGFMLA
                     SFFMLIVLASCLWDRRRGGTRIETSWGQRIATLPALIPPTFIFFVVVGSIYAGWATPT
                     EAASLGVLAALILAAFYRQLTLKMLRAATEGTMRTTAMIMLIILAAQFLNFVLSNIGI
                     TDGVAEAIEKLGLGKYGTLVLIVVFYLILGCFMETISMMILTTPFIAPVVFKLGWDPI
                     WWGIVLTILIEAALITPPVGLNLYVVQGLRNGGAIGDVIQGALPFVGAMLVLILLLMI
                     WPELALWLPDRVMGKGG"
     gene            complement(6209..6724)
                     /locus_tag="PK264_18420"
     CDS             complement(6209..6724)
                     /locus_tag="PK264_18420"
                     /inference="COORDINATES: protein motif:HMM:NF023520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HRD77883.1"
                     /translation="MSEQVPNRLVRVLGGLGAVAALAITLFVANRPPPPEAETAPDNA
                     VLTPNATVVPAPAMQCPEESRLMTRLELVFGMSRKGAEPVSDVEWQEFLDAEITPRFP
                     DGLTVLVGYGQWKGGDGKIAKEASRILLVWHTPDETTEQRIVAIREAWKVRFKQESVL
                     RAESAASCVSF"
     gene            complement(6748..>7794)
                     /gene="tcuA"
                     /locus_tag="PK264_18425"
     CDS             complement(6748..>7794)
                     /gene="tcuA"
                     /locus_tag="PK264_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HRD77884.1"
                     /translation="GHGLVDTQHAICARKGVDIRYETAGHALLTDADGAVIGVKARHK
                     GRTFDLAAAAVVLASGGFESNAEMRCRYMGPGWELAKVRGTRFNTGGGINMALAIGAM
                     PHGHWSSGHAVGWDFNAPPFGDLTVGDNFQKHSYPLGIMVNATGRRFVDEGYDFRNYT
                     YAKYGHVIKDQPDMMAWQIFDKKVLHLLRDEYRIKRVTKVAADTLEELVGKLEGVDAK
                     ACLAELKAYNAAVMTDVPFNPAVKDGRGTRGLAVAKSNWANVLDTPPFEAYQVTCGLT
                     FTFGGLKITTGCEVEHTDGHAIPGLYAAGELVGGLFYHNYPGGTGLTSGAVFGKIAGE
                     GAGAFALGRGQRGG"
BASE COUNT         1282 a         2557 c         2482 g         1349 t
ORIGIN      
        1 gggcagcgag ccaacctgcg acaccgcggg cgtgctcggg cccgccgtcg ccgtccgtcg
       61 ccccgtcagg cagcaacgcc acgatccgcg cgttcgtccg ggccttggcg agcagctcgc
      121 tcgccgccgg aatcttctgg cctaggaagt agcgctcctt cacgatgccg aggtcggaat
      181 cgaccgcgcc cgtcgcgaag acgatgagat cggcgtcttc gggcgcactc accagctcga
      241 cgccacgctc gatcagcaca tgggcgatcg agaccggcac ctcccagctc cgacccgtct
      301 cgaaagcctc ggaaagcttc aactgatggg atggtagccc ggggctgccg agcaggccac
      361 ccagcggacc cgctgcgccg acgagcgtgt cgtagtcacg cgagatgggg agcgctcgaa
      421 aatcgccttc gacgaccacc gacgaggccg gcaggctgcg ccgctcgcgg atctcggtga
      481 tgatcaccgg ccgcgtcgtc gtcggtatga gaaccagggc cttcatgaac ctgccgcgct
      541 gcaagccgcc tcaacgctca gactgtcaga cgccaagccc ccttgggtgg ttcatgggcc
      601 aacacgacgt cgcatatcag atcaggtaaa gctcggttgt ggcttcgcca aggagcttca
      661 gcagtcggga gagatcgccg ttggctggat cgattgcaat ctggatgtgg ccgcgcaccg
      721 cctcggagag tggaattcgg acgctatcgt ccggtccgat cgcctcgaga gcttgggggc
      781 ccgccggccc gccggccgat tgggagagga cgacgaacac gtcgcggcca tcttcgacga
      841 cgcggagatc gaatgcgccg acccggcgcg agggataggt gccggtgcct gcggccatgg
      901 cgagttcgga gtagccgatc gcgctcgact gcagcatgcg ccggtagcgc aggccgactg
      961 tcctgtcgga ccggatcgcc cgctcgatgc ggaagtccat gggtgcgccg ggcccacgca
     1021 gcgcatagcc gatcagctcc gaattggcga tcccggtcgc cgaagccgtg ctctcggcac
     1081 gagtgtcccg catggcggtc acgaggtcgc gggcgttcag ctcggctacg aggtgctgca
     1141 gcatggcggg gctcttgtcg tccttgagtt gcaggggcgt tggtttctgg tctcggtcgt
     1201 ccggcggctt cgtcatgagg aagctcccgg aacagtactt gcgccacctg gagccaaata
     1261 tgcggccctg aacgctgcgg caaatcggac tttatcgtca atgaaacgtg aattcagctc
     1321 tgcaccgcgc tggagattgc cgacgccacc gatgaaaccc tgcacaacct cgcggatctg
     1381 gacgagttcg cccgcgacag gaacgaccga gcgcgcgagc acgtcgggcg cctcgtcgaa
     1441 gccctcgcgc ttcaggcgct tgaggaagcc ctgcccctcg cggcgggcct cggccagcgc
     1501 atcgggctcg acgctcgcga gcgccggatt gtcgttaccg gcgggtgcgg ccaggaaggc
     1561 cgagatcagc agtagcttcg agagatgatc ggcgagcttg acgtagccct tctcgacatc
     1621 tgggtaggcc gtcgcatcgc cgcagtccac gcgcccggcg acgtcgctgc cgccgctgcc
     1681 acggcgcagg aaattggcga tccccgactg cacggcaccg aacgagagat agcgcaacgc
     1741 ggtaagcggc cgcgacaccg tgaagggccc gcattcggcg aggtcggcga ggcggtcacg
     1801 ctcggtaccc gtcagcagct tcacgccttc agggaggccg gtgcgcagga actcaacgag
     1861 ggcctcgtaa cgcagctcga tcggatctcc gatcggctcc acgttctcgg ttgccatttc
     1921 aaccggcgcg agcacgcggc cgtcatcgct atcatagaga gaaacggcat cgttcatggc
     1981 gcgctgggcg ctgacgtcgg ccgcgaccgt ctcgagcgtg aggaaatgct tgaccacggt
     2041 gcgatagagc agccggttgc cctcgcgaat ctggctgtcc cagaaggcga cgatgtcctc
     2101 gtcgtcgaac tggatggcac tcgtgcgccg cggcttggtg ccgagatagc cgaggagcga
     2161 acggaacacc ttctcgtgcc gggcaaccgg aaagcgctcg gtccggtagg cgcggaggcg
     2221 gcttgcgata tcggagacgg cggccttgac ggcggcctca cggccagccg ccggcgcatg
     2281 tgcgagttca ccgatgatgc ggcgcatctc ggcgaagtag ctgcaatcgc tgtgcatcag
     2341 gaggaattcg agaaagccca ttagcgggcc gatctcgtgt gtcggcaact ttcctttgca
     2401 gttcgatacg acggcgccga cgggcccgag aagcacggcg aggcagcggt cgacatcggt
     2461 gcggccaggc ggcttgccag cgaccacctt tgccccgatc tcggcgatga tgcgcacgag
     2521 gtcgaagacg ggccgatcac ggttcggccg gatggcgccg gcgacgaaat tggcgagctt
     2581 cttctggtcg gccgccccga ggcggcgcga ttccacgatg ccattgagct cgttgatcgt
     2641 ctgctgatcg atctgtgcga cgtccacgtc tagacctccg agcgctgccg acggcagcca
     2701 agtgagcaag ggccggccgc atcaaccgga gttgcaggca ccagacagct ttcgaacgca
     2761 cgtcccatgc caatgggcat ggcgaagggc cgtcctgcca tagcacagga taccaccaat
     2821 cgggtcaact cgccgacatg cgaatgaatg aggcaatttc agctgatcaa tagtgctcag
     2881 ggcccgctga ggcctatgcc gccttgccat gggccgagat ttgacggatc gtggcgcgcc
     2941 tggggaaatc cggctcatga caatcgggag gactgaatgc gtagattggc cagcttggcc
     3001 gccttcgcgg cggcgatggc gctttcgact ccactctcgg cccaagggat cgagaagcgc
     3061 tccttcaacg taatcggcac gtggggcaac ctgacgaact ggcagaagct cgaggcgccg
     3121 ttctggaagg aagtgctgcc gaaggcgtcg ggcgacgcgc tgaagatcaa cgtccggtcg
     3181 ctgaccgaag ccaacctcaa gggcaccgag ctgctccgcc tgctgaagca gggcgtctat
     3241 gatttcgcgg ccgtgctgcc gatctatgtc gacgacggcg gcgccgtgat cgaggcctcg
     3301 gatctcgccg gtgtcgcacg cgacttcgcc atggcgcgca agattgccga tctctggctc
     3361 cccgagatgc agaagacgat gggcgacaag cacggcgcca tcatcctcgc gcacttcccc
     3421 ttcccgcagc aggtgttctt ctgccgtggc gacatcaagt ctgtcgccga cctcaagggc
     3481 aagaagatcc gtgtgcaggg cgcctcacag gccgatttcg tgacggccgt tggcggctca
     3541 cctgtgacca tggccttcgg cgacgtgctc ccggctctcg agaagggcgt cgtcgattgc
     3601 ggcatcaccg gaaccatgcc ggcctacaag accaagtggc acgaggtggt cggcacgctc
     3661 ttccagctgc ctgtcggctt cacgctcggc acctggatcg tcaacgccaa gacctggaac
     3721 agcctgccga aggcgacgca ggacctgatc aagaaggaga tggacgcgct caccgaccgc
     3781 tcctggaaaa cgatcgaggc agaatccgat gaaggcgtcg cctgcaatac gggctcaggc
     3841 gcctgctccg cagggtctcc tggcaaggtg aagctcgtga agccatccga tgccgacatc
     3901 gccgcccgcg agaccgcgct caacaccgtc gtactcgcca actgggccaa gcgctgcacg
     3961 ccggattgcg tcgccaagtg gaacgagatc gtcggcaagc cctacaagct caacgcgaag
     4021 taagtcgcga ctgacagcat gcgcgaggct ccccaacgac gtcgcgggga gcctcgttct
     4081 cgcctggggg acttgtacgg gggttcgtta tggaagcgct ttggcgcctc atccagcatt
     4141 tggctcgcat cggcgcctgg gccggcggag gcctgctgat cctcgccgca ctcacggtct
     4201 cgaccgaagt gctgctccgc aaggggctcg gtgcgctcgt cggctatccc atcctgatta
     4261 ccggcacgaa tgaaatcgcc gcctaccttt tcgcaatcgg cacgagttgg tcgctggcct
     4321 acgcgctcgt tacgcgcggg catgtgcgca tcgacacgct ctacgagcgt ttatcgcccc
     4381 atatccgagc catcctcgac atcattgcac tgcttgcgct ggccttgttc gtcgcagtgc
     4441 tcgttgagcg cgcctacgat ctcgtcgccg acagctacct ttccgatcgc cgctccgcga
     4501 cccccctgcg cgtgaaaatg gcgatcccgc agatcgcctg gttcgccggc atcggccggc
     4561 cgaacatcat gatggtcgtc gcgatgttga tcgcgtttct ccgctcgctg ctggcactgg
     4621 ctcatggcga cccaggaacc gtcagccgca ccatcggcgt accaagccag aaagaggaga
     4681 tcaggaacga actcgaaggc ctcggtatcg gccggccggc cggcgcgacc gatccgaccc
     4741 cacccaacgg cccgaaagcc gcgcccactt ctcgcggcaa gcccgccgac tggacctgac
     4801 agatgttagc aacggccctc atcctactcg ctgtcatgct cgcgatcagc gtgccggtcg
     4861 ccgccgttct cggggtactc ggcctttctc tcgatcagtt ttttgcggac gctcgattgc
     4921 gccgcgccat gagcgacatc ctctgggagc agagcgccaa cgacatccta atcgccatcc
     4981 cgatgttcgt gatgctcggc gaggtgctgc tgcgctccgg catcgccgaa cgggtctatg
     5041 cggcgatcgc ccagtggctc tcgtggctgc cgggcggcct cacgcacgcc aacnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac
     5221 tcttgccgcg ggaggcacgc tcggcatcct gatcccgccg tcgatcaact tcatcctcta
     5281 cggcctgctc gtcaaccaga gcgtgcctcg gctctacctc gcgggcttca tcccggggtt
     5341 catgctcgct tccttcttca tgctgatcgt gctcgcctcc tgcctgtggg accgccggcg
     5401 cggcggcacc aggatcgaga cgagctgggg ccagcgcatc gcgacgctgc cggcgctcat
     5461 cccgccaacc ttcatcttct tcgtcgtcgt cggctcgatc tatgccggct gggcaacgcc
     5521 gacggaggct gcctccctcg gcgtgctcgc ggccctcatc cttgccgcgt tctaccggca
     5581 gctcaccttg aagatgctac gcgccgcgac cgaaggcacg atgcgcacca cggcgatgat
     5641 catgcttatc atcctcgccg cgcagttcct gaatttcgta ctctcgaata tcggcatcac
     5701 ggacggcgtc gccgaggcga tcgagaagct cggcctcggc aagtacggca ccctcgtgct
     5761 gatcgtcgtc ttctacctga tcctcggctg cttcatggag accatctcca tgatgatcct
     5821 gacgacgccg ttcatcgcac cggtcgtctt caagctcggc tgggacccca tctggtgggg
     5881 catcgtgctg acgatcctga tcgaggcagc gctgatcacg ccgcccgtcg ggctcaacct
     5941 ctacgtggtg cagggcctgc gcaacggcgg cgcgatcggc gacgtcatcc agggcgcgct
     6001 acccttcgtc ggcgcgatgc tcgtcctcat cctgctgctg atgatctggc ccgagctcgc
     6061 gctctggctc cctgataggg tgatgggcaa gggcggatag cggcagcgcg gccctgacga
     6121 catattgcag gccgggcctc aggcggccaa agccccagtc gggtcggggc ggcagcccgg
     6181 tcgcgaagcg agtgccgcgc cgtcccgatc agaacgagac gcagctcgcc gcgctctcgg
     6241 cgcgcagcac gctttcctgc ttgaagcgca ccttccaagc ctcgcggatc gcaacgatgc
     6301 gttgctctgt cgtctcgtcc ggggtgtgcc agacgagcag gatgcgcgag gcttccttgg
     6361 cgatcttgcc gtcgccgccc ttccactggc cgtagcccac gagcacggtc agcccatccg
     6421 gaaagcgcgg ggtgatctcg gcgtcgagga actcttgcca ttccacgtcg ctcaccggtt
     6481 cggcgccctt ccgcgacatg ccgaagacga gctcgagccg cgtcatcagc cggctttcct
     6541 ccgggcactg catggccggc gcgggtacca cggtcgcgtt gggcgtgagg actgcgttgt
     6601 ctggtgccgt ctcggcctcg ggtggtggcg gacggttggc cacgaacagc gtgatcgcga
     6661 gcgccgcaac ggccccaagt ccgccgagga cccgcaccaa tctgttgggc acctgctcac
     6721 tcatgctcgg ctccaccgct tcgcttctca cccgccccgc tgtccgcggc cgagcgcgaa
     6781 ggcgccggcg ccttcgccgg cgatcttgcc gaacacggcg cccgaggtca gccccgtgcc
     6841 gcccggatag ttgtggtaga agaggccgcc gacgagttct cccgccgcat agaggccggg
     6901 aatggcgtgg ccatcggtgt gctcgacctc gcagcccgtg gtgatcttca ggccgccgaa
     6961 agtgaaggtg aggccgcagg tgacctgata ggcctcgaag ggcggcgtat cgaggacgtt
     7021 ggcccagttt gacttcgcaa ccgcgagccc gcgcgtgccg cggccgtcct tgacggctgg
     7081 attgaacggc acatccgtca tgacggcggc gttgtaggcc ttgagctcgg cgaggcaggc
     7141 cttggcgtcg acgccctcga gcttgccgac gagttcctcg agcgtatcgg ccgcgacttt
     7201 cgtcacgcgc ttgatgcgat actcgtcgcg aaggaggtgc agcacctttt tgtcgaagat
     7261 ctgccaggcc atcatgtccg gctggtcttt gatgacgtgg ccgtacttcg catacgtgta
     7321 gttgcggaag tcgtagccct cgtcgacgaa gcggcgacct gtggcgttca ccataatgcc
     7381 gagcggatag ctgtgcttct ggaagttgtc gccgacggtg aggtcgccga agggcggcgc
     7441 gttgaagtcc cagccgacgg cgtgaccgct cgaccagtgg ccgtgcggca tcgcgccgat
     7501 ggcgagcgcc atgttgatgc cgccgccggt gttgaagcgg gtgccgcgca ccttggcgag
     7561 ctcccagccg gggcccatgt agcggcagcg catctcggca ttggattcga acccgcccga
     7621 ggcgagcacg acggcggccg cggcgagatc gaaggtgcga cccttgtggc gcgccttcac
     7681 gccgatgact gcgccatcgg cgtccgtcag cagcgcgtgg ccggccgtct catagcgaat
     7741 gtcgacgccc ttcctggcgc agatcgcatg ctgcgtgtcg acgaggccgt ggcc
//
LOCUS       DAORNH010000217         7762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6954_length_7762_cov_2.567017, whole genome shotgun sequence.
ACCESSION   DAORNH010000217 DAORNH010000000
VERSION     DAORNH010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6954_length_7762_cov_2.567017"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK264_18430"
     CDS             complement(<1..762)
                     /locus_tag="PK264_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRD77885.1"
                     /translation="MIPRYSRPEMVAIWSPETRFRIWFEIEAAACEGMAEIGMIPKEA
                     AAAIRKRGDKAKFDVAKIDDIERVTKHDVIAFLTHLAEFVGPESRFVHQGMTSSDVLD
                     TCLSVQLVRATDILLGDLDRLLAALKTRAHQHKLTPCIGRSHGIHAEPVTFGMKLAQA
                     YAEFDRAKGRLAAARAEVATCAISGAVGTFANIDPRVEAHVAKALGLSAEPVSTQVIP
                     RDRHAMYFATLAVIASSMERLAIEIRHLQRTEVLEA"
     gene            complement(775..1494)
                     /locus_tag="PK264_18435"
     CDS             complement(775..1494)
                     /locus_tag="PK264_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HRD77886.1"
                     /translation="MTEQIYRTDAYAKAIEAKVVAINEHGGIVLDRTNFYAAAGGQAG
                     DKGVLSFAGRSCPISTTVYDADKATIVHVPAEGAPKPEIGAAVTAELDWATRHAHMRM
                     HSALHLLCSLVKWPVTGGQITAAEGRLDFDIADMAGIDKETLTGKLNALVAADHPIGE
                     RWISDAELDANPSLVRTLSVQPPRGSGRIRLVAVGVDGAIDLQPCGGTHVRSTAEIGR
                     LAVAKIENKGKQNRRIRVVFA"
     gene            complement(1497..>1890)
                     /locus_tag="PK264_18440"
     CDS             complement(1497..>1890)
                     /locus_tag="PK264_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116647.1"
                     /note="CysK; forms a complex with serine acetyltransferase
                     CysE; functions in cysteine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRD77887.1"
                     /translation="YTAGELKSEGSSITEGIGQGRITKNLEGLKVDHSFLIADGEAVR
                     IVFDLLAEEGLCMGSSTGVNVAGAIRLAKALGPGKTIVTILCDYGTRYQSKLFNPEFL
                     RGKSLPVPAWLESRRAPSPNVFVDPEKA"
     assembly_gap    1891..1928
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1929..2548)
                     /locus_tag="PK264_18445"
     CDS             complement(<1929..2548)
                     /locus_tag="PK264_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRD77888.1"
                     /translation="MRYANGVAAAVGNTPLIKLRRASEETGCTILGKAEFMNPGQSVK
                     DRAALFIIRDAERRGLLRPGGTIVEGTAGNTGIGLTVVGNALGYRSVIVIPDTQAQEK
                     KDALRLLGAELIEVPAVPYKNPNNYIKYSARLAEALDRSEPNGAVWANQFDNVANREA
                     HVATTAEEIWEATGGRVDGFVSAVGSGGTLAGTGIGLRAKRPDIQIA"
     gene            2980..>4803
                     /locus_tag="PK264_18450"
     CDS             2980..>4803
                     /locus_tag="PK264_18450"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HRD77889.1"
                     /translation="MRFTPIAPQSASAILSVAARQDQTDLGGLLRTLRRHAGQIALLT
                     LAALLLGLAYLALATPKYTASTSIFIDPRYRKVVNEEVIQGGVGTDLSLVETQVAIIT
                     SDSVMRRVVEREKLSTDPEFVPLRRDGLLSSLRAMILGRPEPVDPVAEAMTALSRAVK
                     VKRAQKTYVVEIEVTSTSPIKAARLADAFAEAYLADQSAAKAAEARRANVLIDGRLGE
                     LREQVRKAEVKVDEFKRSNAIIVSDGGLFNEQTLTRLNNELATARGLVAETKGRLDEI
                     NSMLKAGIGNAESLPDAVKSNLIQRLREQLAAVARREASLAAQLGPRHPVLIEARTQA
                     NELRGQIAAELRRIAAAAKSEHQSAVNREQELVRTLERSKGDTTKTQTAQIRLRELER
                     EFEASREVLRMFLVRAKETREQENITTPDARVITPAAIPSRPSRPISWLVLSLAGLGG
                     LGAGVVRALMGDHLDRSLRTGQQVVSDTGLKALANIPALEPRSGLERMTRRLKGNPAD
                     RVPVSFSDILTSLGDAARPADTPFRQAVLRLLARLRIHGRAGHPQVVMVASGHAETGT
                     SATALALAYAGATSGERTLLVDGCSTDPELSQIFAATLKQTG"
     assembly_gap    4804..4856
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4857..5163
                     /locus_tag="PK264_18455"
     CDS             <4857..5163
                     /locus_tag="PK264_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77890.1"
                     /translation="RSGLSFLPIALADLRRLKLSQRQRLAVGIGKIAQDYDLVIIDAG
                     GLLDDESALSLAPAADQIVLVARAGITERETIAETIQSLEQAKDRVIGLVLTMTEAV"
     gene            5237..6625
                     /locus_tag="PK264_18460"
     CDS             5237..6625
                     /locus_tag="PK264_18460"
                     /inference="COORDINATES: protein motif:HMM:NF038256.1"
                     /note="VpsF, distantly related to oligosaccharide ligases,
                     is encoded next to the probable flippase VpsE; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VpsF family polysaccharide biosynthesis protein"
                     /protein_id="HRD77891.1"
                     /translation="MTEIGRVPSTTLFDAAPPDWNTRGLRMPPSGPLFTAALLAVLLV
                     WSCISAMLLTELGFNYEQPGGLPFSKIHPGTLLAAGVLILAMAATGRPILWLERVIAE
                     RPGVAVFLLATIGMMAHCIILVGAPFTTFIDTFVGPVIAFWLFKDIGDARARMLARII
                     HVVLAVNALVGIAEFASGNRLIPLWIAGELTTSDWRSSALFGHPLSNASITGCYCIIV
                     MLGGGRDIGSIARTFCLAINTAAMVTFGGRAATVMLVAVILLMAAHRFAGYVAGRPFS
                     RAGLVTALLTLPVAIGIVLVLAEAGYFARFVERFIDDQGSAATRIGMFDLLFSFQWQD
                     LLIAPDLKHVETMQRHYGLDYGIESVWIAFIIFYGIIPSIVFFAAMFWFLKDVVRSAR
                     RASFWVVLFFLAVASTSVSLYGKGPQFAILVMHILILLRRDSVPEPYLAERIGLAMSG
                     GTIAGRRRLGAY"
     gene            6625..>7762
                     /locus_tag="PK264_18465"
     CDS             6625..>7762
                     /locus_tag="PK264_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRD77892.1"
                     /translation="MLLRQTLLYLPAQLIGPLFLFVSIVAWTHWLPPHDLGTMTLILV
                     THELMHVVCVSWFSYYVVRYHSRASDADQRRRLIDSEATILALATVASVSASTLVLAR
                     VADGFASPALIVASIAFMATRSITLHLADRARAEDEVVSYTVIQIATSALGLLIGLGL
                     AHVHEPTPLAAIGGLAAAQLLALIACLPRLRPRWNPVRPDASVLKAALAYGLPFIVSG
                     PLIWASVNGLRYIVEFALGSAALGLVTVGWALGQRGASVAALVTTAAAFPLAVKRTRE
                     EGLARGAEQLASNAVLLTLLLAPAMGGLWVLGPQLTNAVVAEAYREATIAVIPLAALA
                     GLVRNIRMHFSNQIFLLHERTDVALRIDAVDAAASLGLALIGLWL"
BASE COUNT         1161 a         2652 c         2474 g         1384 t
ORIGIN      
        1 cgcctccagc acctcggtgc gctgcagatg gcggatctcg atcgcgagcc gctccatcga
       61 ggaggcgatg acggcgagtg tcgcaaaata catggcgtgg cggtcgcgcg ggatgacctg
      121 cgtggagaca ggctcggctg aaagcccgag tgccttcgcc acgtgtgctt cgacgcgcgg
      181 atcgatgttg gcgaaggtgc cgacggcgcc cgaaatggcg caggtggcaa cttccgcgcg
      241 ggccgccgct aaccgccctt tcgcgcgatc gaactcggcg taggcctgcg cgagcttcat
      301 cccgaacgtc accggttcgg cgtggatgcc gtggctgcgc ccgatgcagg gcgtcagctt
      361 gtgctggtgt gcgcgcgtct tgagcgccgc gagcaggcgg tcgagatcgc cgagcaggat
      421 gtcggtcgca cgcacgagct gcacgctcaa gcaggtatcg agcacgtcgg acgacgtcat
      481 gccctggtgc acgaagcggc tctcgggccc gacgaactcg gcgaggtgcg tgagaaacgc
      541 gatcacatcg tgcttcgtca cgcgctcgat atcgtcgatc ttggcgacgt cgaacttcgc
      601 tttgtcgccg cgcttgcgga tggctgctgc ggcctccttg gggatcatgc cgatctcggc
      661 catgccttcg caggcggcgg cctcgatctc gaaccagatg cgaaagcggg tttccggcga
      721 ccagatggcg accatctcag gccgcgaata tcgggggatc atggtgccac ctcttcaggc
      781 gaacacgacg cggatgcgcc ggttctgctt gcccttgttc tcgatcttgg cgacggcgag
      841 ccggccgatc tcggccgtcg agcgcacgtg cgtgccgcca cagggctgca agtcgattgc
      901 gccatcgacg ccgacggcga cgaggcggat gcggcctgat ccgcgcggcg gctgcacgga
      961 gagtgtgcgc accaggctgg ggttggcatc gagctcggca tcgctgatcc agcgctcgcc
     1021 gatcgggtga tcggccgcga caagtgcatt gagctttccg gtcaaggtct ccttgtcgat
     1081 gccggccatg tcggcaatgt cgaaatcgag ccggccttcc gccgccgtta tctgcccgcc
     1141 ggtcacgggc cacttcacga ggctgcacag gagatgcagc gccgaatgca tgcgcatgtg
     1201 ggcgtgacgc gtcgcccagt cgagctctgc cgtcacggcc gcgccgatct ctggcttcgg
     1261 cgcgccctcg gccgggacgt gcacgattgt cgccttgtct gcgtcgtaga ccgtggtcga
     1321 gatcgggcac gaccgcccgg cgaaactcag gacacccttg tccccggcct gaccaccggc
     1381 agcagcatag aagttcgtgc ggtcaagcac gatgccgccg tgctcgttga tggcgacgac
     1441 tttggcctcg atcgctttgg cataggcgtc agtgcgatag atctgctcgg tcatggtcag
     1501 gccttctcag gatcgacgaa tacgttcggc gatggcgccc gtctgctctc gagccaagcc
     1561 ggcaccggca ggctcttccc gcgcaggaac tccgggttga agagcttcga ctggtagcgc
     1621 gtgccgtagt cgcagaggat ggtgacgatg gtctttcctg ggcccaacgc cttggcgagg
     1681 cggatcgctc ccgcgacgtt gacacctgtc gaagagccca tgcagagacc ttcctccgcc
     1741 aggagatcga agacgatgcg cacggcctcg ccatcggcaa tcaggaagga atgatcgact
     1801 ttcagtccct cgaggttctt ggtgatgcgg ccctgcccga tgccctcggt gatcgaggag
     1861 ccctcggact tgagctcgcc cgcagtgtag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnngc gatctggatg tccgggcgct tggcccgcag tccgatcccc gtgcccgcga
     1981 gcgtgccgcc cgagccgacc gcggacacga agccgtcgac cctgccgccg gttgcctccc
     2041 agatctcctc ggccgtcgtt gcgacgtgcg cctcgcggtt ggcgacattg tcgaattgat
     2101 tggcccagac ggctccgttg ggctcgctcc tgtcgagcgc ctcggcgagg cgggccgagt
     2161 acttgatgta gttgttcgga ttcttgtagg ggacggccgg tacctcgatc agctcggcgc
     2221 cgagcagacg gagcgcgtcc ttcttctcct gggcctgagt gtccgggatg acgatcaccg
     2281 agcggtagcc gagcgcgttg ccgacgaccg tgaggccgat acccgtgttg cccgccgtgc
     2341 cctcgacgat cgtgccaccc ggacgcagga ggcctcgacg ctcggcgtca cggatgatga
     2401 acagcgccgc ccggtccttc acggactggc cggggttcat gaactcggct tttccgagga
     2461 tcgtgcatcc ggtctcctcc gaggctcggc ggagcttgat caacggcgtg ttgccgaccg
     2521 ccgccgcgac gccattggca taccgcacgt gcgtctcctt gcttgctgtt tggccggaac
     2581 cgtagtgggt gggtgagagg tgtgcaacct ggagacggcc tggagacaca agatcgcgga
     2641 gcggtgtttc agatgcgttt tcgtgtggcg gcggcctacg acccaggttg accagggtgc
     2701 agcgcagcct cgtcgttgca ggccgcattg cggccgctct cgcttcggca actccccctg
     2761 ttaagccatc ggcacaattc ttgctcgatc cttgccgaat gccgtccgcc ggcatttcgc
     2821 ccggccgcta accagatcgg atcgcgagcg ctgagttgcg gtgccccttc gggtaaaatg
     2881 gtcgtgaact cgagcatcaa agtggcgcaa tcgtgtgtcc cccagatgat cgagggaaat
     2941 cggccggcag ggcgtcgagc cggcaaagag cggttgcaca tgcgcttcac accgatagca
     3001 cctcaatcag cgtccgcgat cctgagcgtg gcggcccggc aggatcagac cgacctcggc
     3061 ggcctcctgc gcacgctccg acgccatgcg ggccaaatcg cattgctgac gctcgccgcg
     3121 ctcctcctcg gcctggcata tctggcactc gccacgccga aatatacggc ctccacgtcg
     3181 atcttcatcg atccgcgata ccgcaaggtc gtcaacgagg aggtcatcca gggcggcgtc
     3241 ggcaccgacc tgtcgctcgt cgagacgcag gtcgccatca tcacttccga cagtgtcatg
     3301 cgccgcgtcg tcgagcgcga aaagctctca accgatcctg aattcgtgcc gctccgccgt
     3361 gacggcctgc tgtcgtcgtt gagagcgatg atcctcggca ggcccgaacc tgtcgatccc
     3421 gtcgccgagg caatgaccgc cttgtcgcgc gccgtgaaag tgaaacgcgc gcagaagacc
     3481 tatgtcgtcg agatcgaggt gacttcgacg agcccgatca aggccgcccg cctcgcggac
     3541 gccttcgccg aggcctatct cgccgaccaa tccgctgcca aggccgccga ggcgcgccgt
     3601 gccaatgtcc tcatcgacgg ccggctcggc gagctgcgcg agcaggtgcg caaggccgag
     3661 gtcaaggtcg acgagttcaa gcgctcgaac gcgatcatcg tctcggatgg tggcctcttc
     3721 aacgagcaga cgctgacccg tctcaacaac gagctggcga ccgcccgcgg cctcgtcgcc
     3781 gaaaccaaag gccggctcga cgagatcaac tccatgctga aagccggcat cggcaatgcc
     3841 gaaagcctgc cggacgccgt aaaatccaat ctcatccagc ggctcaggga gcagcttgcc
     3901 gccgtggctc gacgcgaggc atcgctcgcg gcacagctcg ggccgcggca tcccgtcctg
     3961 atcgaggcgc gcacgcaggc caatgagctg cgtgggcaga tcgcggcgga gctgcgccgc
     4021 atcgcggccg ccgccaagag cgagcaccag agcgcggtca accgcgagca ggagctcgtc
     4081 cgcacgctcg agcgctcgaa gggagatacc acgaagacgc agaccgccca gatcaggctc
     4141 cgcgagctcg agcgtgagtt cgaggcgagc cgcgaggtgc tgcgcatgtt cctcgtgcgc
     4201 gccaaggaga cgcgcgagca ggagaatatc accacgccgg acgcgcgcgt catcacgccg
     4261 gctgccatcc cctcgcgccc gagccggccg atcagctggc tcgtcctgtc gctcgccggt
     4321 ctcggcggtc tcggggccgg cgtcgtccgc gcgctcatgg gcgatcacct ggaccgcagc
     4381 ctgcgcacgg ggcagcaggt cgtgagcgac acgggtctca aggcactcgc aaacattccg
     4441 gcgcttgagc caagatccgg cctcgagcgc atgacgcgcc ggctcaaggg caacccggcc
     4501 gacagagttc ctgtgtcgtt cagcgacata ctgacatcgc tcggtgatgc cgcgcggccg
     4561 gccgatacgc cgttccgcca ggctgtcctg cgcctcctcg cgcggctccg catccatggg
     4621 cgcgccggcc acccgcaggt ggtgatggtc gcgtccggcc acgccgagac gggcacgtct
     4681 gcaaccgcgc tggcgctcgc ctatgccggg gcgacctcgg gcgagcggac gctgctcgtc
     4741 gacggctgct cgaccgatcc ggagctctcg cagatcttcg ccgcaaccct gaagcagacc
     4801 ggtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgc
     4861 tcgggcttgt cgttcctgcc gatcgcgctc gctgatcttc gccgcctgaa gctcagccag
     4921 cgccagcggc tcgcggttgg catcggcaag atcgcccagg actatgatct cgtgatcatc
     4981 gatgcgggcg ggctgctcga cgatgaatcg gccctcagcc tcgcgccggc ggcggaccag
     5041 atcgtgctcg tcgcgcgggc cggcatcacg gagcgcgaga cgatcgccga gaccatccag
     5101 tcgctcgagc aggcgaagga tcgcgtgata gggctggttc ttacaatgac ggaggccgtc
     5161 tgataccgga ggggccgacc gggccacatc ttccgacacg tgcacgccct ctcgcggacc
     5221 gacaggaccc gacgacatga cagagatcgg ccgggtgccg tccacgaccc tcttcgatgc
     5281 cgctccgccg gactggaata cgcgcggcct ccgcatgccg ccatcgggtc cgctcttcac
     5341 ggccgcgctg ctcgccgtgc tcctggtgtg gagctgcatt tccgcgatgc tcctgaccga
     5401 gcttggcttc aactacgagc agccgggcgg gcttccgttc tcgaagatcc atcccggcac
     5461 tctgctcgcc gccggtgtac tcatcctcgc catggctgcc acgggacgcc ccattctctg
     5521 gctcgagcgc gtgatcgccg agcgtccggg cgtggcagtc ttcctgctgg caacgatcgg
     5581 catgatggcg cactgcatca tcctggtcgg cgcgccgttc acgaccttca tcgacacctt
     5641 cgtcgggccg gtgatcgcct tctggctctt caaggatatc ggcgatgccc gcgcgcgcat
     5701 gctggcgcgc atcatccacg tggtcctcgc cgtcaacgcg ctcgtcggca tcgccgagtt
     5761 cgcgagcggc aaccggctga tcccgctctg gatcgcgggc gagctaacga ccagcgactg
     5821 gcgctcttcg gcgcttttcg gtcacccctt gtcgaatgcc tcgatcacgg gctgctactg
     5881 catcatcgtc atgctcggag gcggtcgcga catcggctcg atcgcccgca cgttctgcct
     5941 cgccatcaat actgccgcga tggtgacgtt cggcggccgc gccgcaaccg tcatgctggt
     6001 cgccgtcatc ctgctgatgg ctgcccatcg ctttgccggc tatgtcgccg gccggccctt
     6061 cagccgtgcg gggctggtga cggcactcct gacactgccg gtcgcgatcg gcatcgtgct
     6121 cgtgctcgcg gaggccggtt acttcgcgcg cttcgtcgag cgcttcatcg atgaccaggg
     6181 cagcgccgcc acccgcatcg gcatgttcga cctgctcttc tcgttccagt ggcaggacct
     6241 gctcatcgcg ccggacctga agcacgtcga gacgatgcag cgtcactatg gcctcgacta
     6301 cggcatcgaa agtgtgtgga tcgcattcat catcttctac ggcatcatac cgagcatcgt
     6361 cttcttcgcc gccatgttct ggttcctcaa ggatgtggtg cgctcggccc gccgcgccag
     6421 cttctgggtc gtgctgttct ttctcgcggt tgcctcgacg tcggtctcgc tctacgggaa
     6481 gggcccgcag ttcgcaatcc tcgtgatgca catcctgatc ctgctccgac gcgatagcgt
     6541 tcccgaaccc tacctcgccg agcggatcgg cctcgccatg tccggcggca cgatcgctgg
     6601 ccgccggcgg cttggggcct actgatgctc cttcgccaga cgcttctcta cctgccggca
     6661 cagctgatcg ggccgctgtt cctgttcgtc tcaattgttg cctggacgca ctggctcccg
     6721 ccgcacgacc taggcaccat gacgctgatc ctcgtcacgc acgagctcat gcatgtcgtc
     6781 tgcgtgagct ggttcagcta ctacgtggtg cgctaccact cgcgggcctc cgacgcggac
     6841 cagcgccgcc gcctcatcga cagcgaggcg accatcctcg cgctggcgac cgtcgcttcg
     6901 gtcagcgcct cgacgctcgt cctcgcacgc gtcgccgacg gcttcgcctc gccagccctg
     6961 atcgtcgcct cgatcgcctt catggcgacg cgctcgatca ctttgcatct cgccgaccgg
     7021 gcgcgcgccg aggacgaggt ggtgagctat acggtgatcc agatcgcaac gagcgcgctc
     7081 ggtctgctca tcggcctggg gcttgcccac gtccatgagc cgacaccgct cgccgcaatc
     7141 ggcgggctcg cggcggcgca gctcctcgcc ctcatcgcct gcctgccgcg cctgcggccc
     7201 agatggaacc cggtgcgccc cgacgcttcc gtgctcaaag cggccctcgc ctacggcctg
     7261 ccgttcatcg tcagcggccc gctcatctgg gcgtcggtca atggactgcg ctacatcgtc
     7321 gagttcgcac tcggctccgc ggcgctcggc cttgtcacgg tcggctgggc gctcgggcag
     7381 cgcggggctt cggtcgcggc gctggtgacg acggcggccg ccttcccgct tgcggtgaag
     7441 aggacacgcg aggagggtct cgcacgcggt gccgagcagc tcgcgagcaa cgccgtgctg
     7501 ctcacgctcc tgctcgcgcc tgccatgggc gggctctggg tgctcgggcc tcagctgacg
     7561 aacgcggtcg tcgccgaggc ctatcgcgag gcgacgatcg ccgtgatccc gctcgccgcc
     7621 cttgccgggc tcgtccgcaa catccgcatg cacttctcga atcagatctt cctgctgcac
     7681 gagcggactg acgttgcgct caggatcgat gccgtggacg ctgcagcgag cctcggtctc
     7741 gccctcatcg ggctctggct cg
//
LOCUS       DAORNH010000218         7750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6965_length_7750_cov_2.725016, whole genome shotgun sequence.
ACCESSION   DAORNH010000218 DAORNH010000000
VERSION     DAORNH010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6965_length_7750_cov_2.725016"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK264_18470"
     CDS             complement(<1..370)
                     /locus_tag="PK264_18470"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRD77893.1"
                     /translation="MPKSMPLKPEAQGLNPKASFQDLILTLQQFWGRQGCVVLQPYDM
                     EVGAGTFHPATTLRALGPKTWNAAYVQPSRRPKDGRYGENPNRLQHYYQFQVIMKPSP
                     PDILDLYLKSLDAIGLDTREN"
     gene            590..2518
                     /locus_tag="PK264_18475"
     CDS             590..2518
                     /locus_tag="PK264_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRD77894.1"
                     /translation="MSKPAMKVTQVSDAATGTPPGSPRREPARSAKAPKASGAADIFV
                     LAAIAAVSMAIGLGSYAQLGCGIGVAACLAMAALLALSSLHVALRRSQSLAELTAENA
                     RLKSKVEGLVRVRRMMPQAPAAKVPTAAAHEPRMPADAVAAEGLPSVAAQAPPAPASL
                     VPTPAEEPTDASPPSEADLVQSIASVKEQWALRPGFARAPALAGPSGVSDEASRGDDT
                     TATGSVPLADGAIVVAAPPVRAEAPAEARISTSRVQTVTAPSSPTAASAQPSDAAAVE
                     NPRKPRADFDAIQALIKRLATDLNAPRKPEPPEEPSSPATEPAETAADPQVHAQDAIT
                     RSVVALRHTGAAMRRAEMPDEAIALQVGRQAAVPAAPPSAPAAGPQSPPPLDPVPSVS
                     LIDAITEAVAADRYDVMVEPIQSLKDRKARHFEIGLRLIIQNGTALDDVDYVRATRGT
                     ELLARIDAAKMLRTARFAEHLLTRGKSASVHSAISGDSLASDAFLDDFAATLEGRGQL
                     AHVLVLGFSQIEVRSFSPVHWETLHVMADLGLRFSLEHVTDLDMDFESLKGSGFEFVK
                     LDAEVFLDGLQCEGTLVPAADLCRHLSELGLSLVVSHIADDRALAKILGFGVLLGQGE
                     LFGRPRRIDMDGSARVAA"
     gene            complement(2525..3328)
                     /locus_tag="PK264_18480"
     CDS             complement(2525..3328)
                     /locus_tag="PK264_18480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD77895.1"
                     /translation="MPARRLPPLHAVRAFEAAARHLSMTDAAAELNVTPGAISRHVRA
                     LEAEMQTPLFLRRSTGLVLTSAGEALADVARDALDGLANAVSGLRLRRFRRLSVGAYG
                     FFISRAVLPHWRELMAGHPELEIDLHVSSNALDLVPGRHDAVIAVSDGTPRPGSITHR
                     LMPIATVPVCAPSWLREGRADFASLPLLHARPRPEDWRRWLDFAGYGFVSAQSDSSFE
                     SVGLALEAASAGMGSAMAIEGLLAADLTLLNLVRADNRARPTRRVACHV"
     gene            3455..4240
                     /locus_tag="PK264_18485"
     CDS             3455..4240
                     /locus_tag="PK264_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRD77896.1"
                     /translation="MTTDRDQREMARLLAAARRDARQIRDLDACLVPATVAEAYRVNA
                     LVEDALGWESLGWKIAATTEAVRTRLGTPGPIYGRTFRRFRHASPARFNHADLLDPLV
                     ECEFFVTLARDLPPRDRAWSMTDIIAAIASVHAGIEVAECRFPMRALPPLPAILADGS
                     ASGRYVFGDEIANWRVDLPQVEVRLEVDGREIRRGSGADVMGDPLRPLLWLAEERRAF
                     GDGLRAGETISTGSMTGMLPIRPGQHVLARFGASAIVEIAFTA"
     gene            4281..6077
                     /locus_tag="PK264_18490"
     CDS             4281..6077
                     /locus_tag="PK264_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD77897.1"
                     /translation="MSTAITRSEPTPSALRAGTARRSEHVAPSCRGLNFFEIDRSLQS
                     LLPLYMDAPLLSHLTPHLNELGALGGGRLGALSDQAERHTPILHHRDPFGRDEEWVEF
                     HPAYKAMEEIAFGRYGMHAMSHKPGILGWPSIMPPIAKYMFHYLFAQAEFGLLCPVNV
                     TDSSSELVHRFGSPAIKAKFLDRMWSQDMEQIFKSAQFMTEKAGGSDVGAAELTAVRD
                     GDHWRLYGEKWFCSCVDADVAVLLARPKGAPDGGRGLGLFLMPKTLDDGSRNAYRIMR
                     LKDKLGSKAMASGEIVFEGAIAYQLGELDQGLKHMLVMVNSSRISHLTRAAGMMRRCL
                     NEALVASRHRNAFGRAVIEHPLMRRQLLKLMLPTEQALSALMYSAAATGQAKHDSSAE
                     LILRMLTPIAKYRACRDNVTVATGAMEARGGNGYIEDWPNARLVRDAHLGLLWEGTSN
                     INALDAVQRAVGKVGAHRALEADLGRQLDAADGIPGQFRTRLTRGVADAFRLAEEVAS
                     DPGNERFCRLAAGQLYHATTAALLAAEGQKLGQSGGDARRLLLARLVLEHRLKSSQAT
                     SLASLKWEEAIADRLLADAPVTLEDARALLVA"
     gene            6103..6879
                     /locus_tag="PK264_18495"
     CDS             6103..6879
                     /locus_tag="PK264_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD77898.1"
                     /translation="MPSELIRYSVADEVAEIVLDRGPVNALSMVLIEALLASLDRARN
                     DPGVRAVVISSAHKVFCAGLDLDIVRGKGPLETKRFLERLYFALNDIQYRLGKPSIAA
                     IDGPARAGGMTIAISCDMIIAGRGSTFGYPEVDVGLIPALHFVQLPRLVGKHQAFAPL
                     FLGEPFDAETAFRMGLLSEIVETGSALTRARVLAQKFREKSPVVLKLGREAFMRAVDA
                     DYRRSVENVAETFSLVATTEDCQEGLTAFVEKRKPKYKGQ"
     gene            complement(7080..7376)
                     /locus_tag="PK264_18500"
     CDS             complement(7080..7376)
                     /locus_tag="PK264_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRD77899.1"
                     /translation="MREHDYFVYILASGRNGTLYVGVTNDIVRRVAEHREDLVEGFTK
                     KYGVKQLVYFEPHGEILEAIAREKRIKRWRRAWKIELIETGNPEWRDLWHELIA"
BASE COUNT         1255 a         2836 c         2424 g         1235 t
ORIGIN      
        1 cgttctcgcg cgtgtcgagg ccgatggcat cgaggctctt caggtagaga tccaggatgt
       61 cgggcggcga gggcttcatg atcacctgga actggtagta gtgctgcagt cggttggggt
      121 tctcgccata gcgcccatcc ttcgggcggc gcgagggctg cacgtaagcc gcattccagg
      181 tcttggggcc gagcgcacgc agcgtcgtcg cggggtgaaa cgtgcccgcg ccgacctcca
      241 tgtcgtaggg ctggagcacc acacagccct gccggcccca gaactgctgg agtgtcagga
      301 tcaggtcctg gaacgaggcc ttgggattga ggccttgcgc ttcgggcttc agcggcatcg
      361 acttcggcat gaggcattcc tcgttgcagc cggcgcacta tagggttttc ggcaggcctg
      421 tcgacaccac cgtcgcgtgg gcagctcgcg acctctcgac ccttccgtgc ctcgagcaaa
      481 caggggaaat tgcatcagag ccacaccgcc tccggttgca ttcgctggcg ttgccgaacg
      541 acgatcgcgt gagcgttgat tgattcgcgc cgagggaagg cggaacggca tgtcgaagcc
      601 agccatgaaa gtcacacagg tatccgatgc tgcaaccggc accccgccgg gttcgccaag
      661 gcgagagccc gcgcgatccg cgaaagcgcc gaaagcctcg ggcgcagccg acatcttcgt
      721 gctcgccgcc atcgcggccg tcagcatggc gatcggcctt ggcagctacg cccagctcgg
      781 ctgcggcatc ggagtcgcag cgtgcctcgc catggctgcg ctactggcac tctcgagcct
      841 gcacgtcgct ctccgccgga gccagtcgct cgccgagctc acggccgaga acgcgcggct
      901 gaagtccaaa gtcgagggcc tcgtgcgggt tcgtcgcatg atgccgcagg ccccagcggc
      961 aaaagtgccg accgctgccg cacacgagcc tcgcatgcct gccgacgccg tggccgccga
     1021 aggtcttcct tcagtcgccg cgcaggcgcc acctgcgcca gcgtcactcg tgccgactcc
     1081 ggccgaagaa ccgacggacg ctagcccgcc gtccgaggcc gacctagtcc agtccatcgc
     1141 cagcgtcaag gaacagtggg cgctgcggcc cggtttcgcc cgcgccccgg cactcgccgg
     1201 cccatcaggc gtgagcgacg aggcgtcgag gggcgacgac acgacggcga ctggatcggt
     1261 gccactcgct gacggtgcca tcgttgtcgc ggctccgccg gtacgagcgg aagcgccggc
     1321 cgaggcgcgc atcagcactt cgcgggttca aactgtgact gcgccgagct cgccgacggc
     1381 agcttcggcg caaccgtccg acgctgccgc cgtagaaaac ccgcgaaagc cgcgcgccga
     1441 cttcgatgcc atccaggccc tgatcaagcg cctcgcaacc gatctcaatg cgccccgcaa
     1501 gcccgaaccg cctgaggagc ccagcagccc tgcgacggaa cctgccgaga cggccgccga
     1561 cccgcaggtc cacgcccagg acgcgatcac acgctccgtc gtggcgttgc gacacaccgg
     1621 agctgccatg cggcgcgccg agatgccgga cgaggccatc gcgcttcaag tcgggcgaca
     1681 ggcagcggtt ccggccgctc cgccgagcgc gcccgcagcc ggaccgcaat ctccgccgcc
     1741 ccttgacccg gtgccatcgg tgagcctcat cgatgccatc acggaagcgg tcgcggctga
     1801 tcgctacgac gtcatggtgg agccgatcca aagcctcaag gatcgcaagg cgcgccactt
     1861 cgagatcggt ctcaggctga tcattcagaa tgggacggcg ctagacgatg tcgactatgt
     1921 tcgcgccacc cgaggcaccg agctcctcgc tcgcatcgat gccgccaaga tgctgcggac
     1981 ggcgcgcttc gccgagcacc tgctcacgcg cgggaagagc gcatccgtgc attcggcaat
     2041 ctcgggtgac tcgctcgcga gcgacgcctt cctcgatgat tttgccgcaa cactggaggg
     2101 ccgcgggcag ctcgcccatg tcctcgttct cggcttttcg cagattgaag tccgttcgtt
     2161 ctcacctgtg cattgggaga ccttgcacgt catggccgat ctcggcctgc gcttctcgct
     2221 cgagcatgtc accgacctcg acatggactt cgagagcctg aagggctccg gcttcgagtt
     2281 cgtaaagctc gatgccgagg tgttcctcga cggcttgcag tgcgaaggca cactggtccc
     2341 cgctgccgac ctctgccggc acctctccga gctcggactg tctctcgtcg tgagccacat
     2401 tgctgacgat cgggcgctcg cgaagatcct gggcttcggc gtattgcttg gccagggcga
     2461 gctgttcggc cgcccgcggc ggatcgacat ggacggcagc gcgcgcgtcg ctgcctgagc
     2521 tgcctcatac atggcacgca acgcgccgcg tcggcctggc ccgattgtcg gcccgcacca
     2581 gattgagcag ggtgaggtcc gccgcgagca acccctcgat cgccatcgct gatcccatcc
     2641 cggcagaggc agcctccagg gcgaggccga cgctctcgaa gctgctgtcc gattgcgcgc
     2701 tcacaaagcc gtagcccgca aaatcgagcc atctccgcca gtcctcgggg cgaggccggg
     2761 cgtgaagcag cggcagagaa gcaaagtccg ctctgccctc gcgcagccaa gacggcgcac
     2821 agaccggtac tgtcgcgatc ggcatcagcc gatgcgtgat gctgccgggg cgcggggtgc
     2881 cgtccgagac tgcgatcacg gcgtcatggc gccccggaac gagatcgagc gcgttcgagc
     2941 tgacgtgcag atcgatctcc agctccggat gcccggccat gagctcccgc caatgcggca
     3001 gcacggcccg ggagatgaag aagccgtagg caccgactga caagcgccga aagcgcctga
     3061 gcctcaatcc gctcaccgcg ttggcaaggc catctagcgc atcccgggcg acatcggcga
     3121 gcgcctcacc cgccgaggtc agcacgaggc ccgtcgagcg cctgagaaac agcggcgtct
     3181 gcatctcggc ctcaagcgcc ctgacatgcc ggctgatggc gccgggcgtc acgttcagct
     3241 ccgcggcggc gtccgtcatc gacagatggc gggccgcggc ctcgaaggcg cggacggcat
     3301 gcagcggtgg caggcgtcgt gcgggcatat cttgagctag actcaaatcg gtctgcaaac
     3361 aactcgtttg accagcatct ggacgcactc ctatgcatga tccgggatcg ccatcgctgc
     3421 cggcccttgg ctcgcgtaat ttggatcgta tccgatgacc actgatcgcg accaacgtga
     3481 gatggcccgc ctgctcgcag cggcgcgtcg ggatgcgcgc caaatccgcg atctcgacgc
     3541 ttgcctcgtc cccgcgaccg tcgccgaagc ctatcgcgtc aatgccctgg tcgaagacgc
     3601 gcttggatgg gaatcgctcg gctggaaaat tgcggcgacg accgaggcgg tgcgaacgag
     3661 gctcggcacg cctggaccga tctacggtcg cacattccgc cgctttcgcc atgcctcgcc
     3721 cgctcgcttc aaccacgcag acctgctcga cccgctggtc gagtgcgagt tctttgtcac
     3781 gctcgcccgc gatcttcctc cgcgcgatcg cgcctggtct atgactgaca tcatcgccgc
     3841 catcgcaagc gtgcacgctg gcatcgaggt cgccgagtgc cgcttcccga tgcgcgccct
     3901 gccgccgctt cccgcgatcc tcgccgacgg cagcgcctcg ggccgctacg tcttcggaga
     3961 cgaaatcgcc aattggcgcg tggatctccc ccaagtcgag gtccgcctcg aggtcgacgg
     4021 ccgcgagatc cgccgcggct ccggcgccga cgtgatgggc gatcccctcc gtccgctcct
     4081 ctggctcgcc gaggagcgcc gggcgttcgg cgacggcctc cgcgccggcg agaccatttc
     4141 caccggctcg atgacgggca tgttgccgat ccggcccggc cagcacgtct tggcgcgctt
     4201 tggcgcctcc gccatagtcg agatcgcctt tactgcctga caaaccctgc cacgcccttc
     4261 gccctcccag gagccccgcc atgtccacgg caatcacccg ctcagagccc acgccatccg
     4321 ccctccgagc cggcaccgcg cggcgttccg agcatgtcgc cccgagctgc cgcgggctca
     4381 actttttcga gatcgaccgg agcctgcaga gcctcctgcc gctctacatg gacgcgcccc
     4441 tgctctcgca cctcactccc cacctcaacg aactcggcgc gctcggcggc ggccgcctcg
     4501 gcgccctctc cgatcaggcc gagcggcaca cgccgatcct gcatcaccgc gaccccttcg
     4561 gccgcgacga ggagtgggtc gagttccacc ccgcctacaa ggccatggag gagatcgcct
     4621 tcggccgcta cggcatgcac gcgatgtccc acaagccggg catcctcggc tggccgtcga
     4681 tcatgccgcc gatcgccaag tacatgttcc actatctctt cgcgcaggcc gagttcggcc
     4741 tcctctgccc ggtcaatgtc accgacagct cgtccgagct cgtgcaccgc ttcggcagcc
     4801 ccgcgatcaa ggccaagttc ctcgaccgca tgtggtcgca ggacatggag cagatcttca
     4861 agagcgcgca gttcatgacc gaaaaggccg gcggctccga cgtcggcgcg gcggagctca
     4921 ccgcggtccg cgacggcgac cactggcgcc tctacggcga gaaatggttc tgctcctgcg
     4981 tcgatgccga cgttgccgtg ctgctcgcgc ggcccaaggg cgcgccagac ggcggcaggg
     5041 gcctcggcct cttcctgatg ccgaagacgc tcgatgacgg ctcgcgcaac gcctatcgca
     5101 tcatgcgcct caaggacaag ctcggcagca aggccatggc gagcggcgaa atcgtcttcg
     5161 agggcgcgat cgcctatcag ctcggcgagc tcgaccaggg cctgaagcac atgctcgtga
     5221 tggtcaactc gagccgcatc tcgcatctga cgcgcgccgc cggcatgatg cgccgctgcc
     5281 tcaacgaggc gctcgtcgcg agccgacacc gtaacgcctt cggccgcgcg gtgatcgagc
     5341 atccgctgat gcgccgccag ctcctgaagc tcatgctgcc gacggagcag gcgctctccg
     5401 cgctcatgta ctcggccgcc gccaccggcc aggcgaagca tgactcctcc gccgagctga
     5461 tcctgcgcat gctcaccccc atcgccaagt accgcgcctg ccgcgacaac gtcacggtcg
     5521 cgaccggcgc gatggaggcg cgcggcggca acggctacat cgaggactgg ccgaacgcgc
     5581 gcctcgtgcg tgacgcgcac ctgggtctcc tctgggaggg aacgagcaat atcaatgcgc
     5641 tcgatgccgt gcagcgtgcc gtcggcaagg tcggcgcgca ccgcgcgctc gaagcggacc
     5701 tcggccgcca gctcgacgcg gcggacggca ttcccggcca gttccgcacg cgcctgacgc
     5761 gcggcgtcgc cgacgccttc cgcctcgccg aggaggtcgc ctccgatccc gggaacgagc
     5821 gcttctgtcg tctcgccgcc ggccaactct accacgcgac gaccgccgcg ctgttagctg
     5881 ccgaaggcca gaagctcggc caatccggcg gcgatgcccg ccgtctgctg ctcgcccgtc
     5941 tcgtgctcga gcatcgcctg aagtcgagcc aagccacgag cctcgcgagc ctcaagtggg
     6001 aggaggcgat cgccgatcgc ctgctcgcgg acgcgcccgt cacgctcgag gacgcacgcg
     6061 cgctgctcgt cgcctgagcc cgcagccaga ggagacgcac ccatgccatc cgagctcatc
     6121 cgctattcgg tcgccgacga ggtcgccgag atcgtcctcg accgcggccc cgtcaacgcg
     6181 ctcagcatgg tgctgatcga ggcgctgctc gcgagcctcg atcgcgcgcg caacgacccg
     6241 ggcgtccgcg ccgtcgtcat ctcgagcgcg cacaaggtct tctgcgccgg cctcgacctc
     6301 gacatcgtgc gcggcaaagg cccgctcgag acgaagcgct ttctcgagcg gctctacttc
     6361 gcgctgaacg atatccaata ccgcctcggc aagccgagca tcgccgcaat cgacggcccc
     6421 gcgcgcgccg gcggcatgac gatcgccatc tcctgcgaca tgatcatcgc cggccgcggc
     6481 tcgaccttcg gctacccgga ggtcgacgtc ggcctcatcc cggccttgca cttcgtgcag
     6541 ctgccgcgtc tcgtcggcaa gcatcaggcc ttcgcgccgc tcttcctcgg cgagccgttc
     6601 gatgccgaga cggccttccg catggggttg ctcagcgaga tcgtcgagac cggcagcgcg
     6661 ctcacccgcg cccgcgtgct tgcgcaaaaa ttccgcgaga agtcgcctgt cgtcctgaag
     6721 ctcggccgcg aagccttcat gcgcgccgtc gacgccgact accgccgctc ggtcgagaac
     6781 gtcgccgaaa ccttctccct cgtcgccacc accgaggact gccaggaagg cctcacggcc
     6841 ttcgtcgaga agcgcaagcc gaagtacaag ggccaatgaa gcgcgcatgg ctgtcatccc
     6901 ggacgccgga gcggcccttc tgccgcgcag gcgatccggg acccagcagc aagagcggcg
     6961 cagctgccgt atctcttcca cccgaaatac cgagcgctgc gcgcactcgt cacgctgggt
     7021 cccggctcgg cgcgttcgcg tgcgctcacg cttggccggg atgacagggg tggggcgcgt
     7081 caggcgatca gctcatgcca aagatcgcgc cattccggat tgcccgtctc gatcagttcg
     7141 attttccagg cccgccgcca acgcttgatc cgcttttcgc gcgcaatcgc ctcgagaatt
     7201 tccccgtgcg gctcgaaata gaccagttgc ttcacaccat actttttcgt gaacccctcg
     7261 acgaggtcct cgcgatgctc tgccactctg cgcacgatgt cgttcgtgac gccgacatag
     7321 agcgtcccat tgcgaccgct ggcaaggatg tacacgaagt agtcatgctc ccgcatatcg
     7381 ccatcctcgc tcagtccagc gccaccaccc atccctgtca tcccggacgc cgaagcggca
     7441 cttctgccgc gcaggcgagc cgggaaccag cggaaagagc ggcgcagctg ccgcctccct
     7501 tcacccgcaa tatcgagcgc tgcgcgcgct cgtcacgctg ggtcccggct cggcgcgctc
     7561 gcatgcgctc gcgcttggcc gggatgacag cgacgccgcg cccgcatccc tgtcatcccg
     7621 gacaccggag cggcacttct gccgcgaagg cgagccgggg ccccgcggca tgagcggcga
     7681 agccgccgca tcccttcacc cgcaatatcg agcgctacgc gcgctcgtcg cgctgggtcc
     7741 cggctcggcg
//
LOCUS       DAORNH010000219         7727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_6989_length_7727_cov_2.540146, whole genome shotgun sequence.
ACCESSION   DAORNH010000219 DAORNH010000000
VERSION     DAORNH010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6989_length_7727_cov_2.540146"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..1389)
                     /locus_tag="PK264_18505"
     CDS             complement(346..1389)
                     /locus_tag="PK264_18505"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRD77900.1"
                     /translation="MNRRRLLQTAIAAGTLAPSSTRATGPIHISDMHFHSFFGASINH
                     SRPVAKSLADGGATLVSWSVAGDALWMDPKTYRQSKEQRPGEALGWVEREIGRIKAHS
                     AEQGLKLALTAADVERAEKGEPHIVLSIEGANFIEGDASRVKRAYDLGVRHLQLVHFT
                     ANTLGDFQTEAPVHGGLTETGRQVVAECNRLGILVDLAHAAGPTVTQTLAVAKAPLVW
                     SHGSVKMGAAAPWNAVIWRARQLPLDLAKAIAAKGGVVGLWALSFDVGKTIDGYGNRM
                     LELADWLGDRHVAFGTDINGLSGNAAIRSYVDLRAVVSGWQTRKVPEARIRRLASENY
                     ARVLKEALVPARG"
     gene            1592..1873
                     /locus_tag="PK264_18510"
     CDS             1592..1873
                     /locus_tag="PK264_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77901.1"
                     /translation="MRSTYLIAAVVALAIPLAAMPWSETDVLAQDDKAKRKKSNSKSS
                     DRKPSPPPASKSSGGYPSCNQGVLWVDTLCRLDDGRVCLVGEFDITDCK"
     gene            complement(1902..2405)
                     /locus_tag="PK264_18515"
     CDS             complement(1902..2405)
                     /locus_tag="PK264_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77902.1"
                     /translation="MGGGLERDYLVFGGLALVSFLLGWKIKRDKSVVAYLFVLAILIP
                     VSGMIAGMWDAPSARAYRHFRRPGLDPAHIMLLITLPYVLAGFLGWLFKPGRHPGTPA
                     TRVARPFAATGAPAPASLARQTLRRVVVTVASAVISYGILSFVGLADMPSILTSVGIA
                     LAALVLG"
     gene            complement(2416..2859)
                     /locus_tag="PK264_18520"
     CDS             complement(2416..2859)
                     /locus_tag="PK264_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77903.1"
                     /translation="MTEAQLRHLIDDPLPAGVYDAAEAAIVRYAQKSTRLEQIDAQTY
                     SALERHFSPAQIVDICLTVGLSNMVNRFHLTFLTEVDASTTAAAEAGDASAGACLCLE
                     HAVSAWLERAASRRVARWLETRHGGIPFADAMRFAALAGGGELFL"
     gene            complement(2896..3147)
                     /locus_tag="PK264_18525"
     CDS             complement(2896..3147)
                     /locus_tag="PK264_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77904.1"
                     /translation="MARIPLHDPADPAVSRDTRAALEAAARSRGALLNIHRAMANRPE
                     ALGAFLNFVETVYRGRSTLAPRHGELAYLTATAVNNCHY"
     gene            complement(3169..4392)
                     /locus_tag="PK264_18530"
     CDS             complement(3169..4392)
                     /locus_tag="PK264_18530"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRD77905.1"
                     /translation="MVQEFHRIKRLPPYVFAEVNRLKAEARARGADIIDLGMGNPDLA
                     VPAHILEKMTETVGKPRTDRYSASKGIPGLLRAQAGYYGRRFGVKLKPESQVVATLGS
                     KEGFANMAQAITAPGDVVLVPNPSYPIHAFGFLMAGGVIRSVPSEPTPEFFTALERAL
                     IHSIPKPIAVVVCYPANPTAYVATIDFYRDLVQFAKKHDIIILSDLAYAEVYFDEKNP
                     PPSVLQVPGAMDVTVEFTSMSKTFSMAGWRMGFAVGNERLLAALARVKSYLDYGAYTP
                     IQVAAAAALNGPTECIDEMRTTYKRRRDVLVESFTNAGWPFPAPSASMFAWVPVPKAF
                     EALGSLEFSKLLVEKASVAVAPGIGFGEHGDGYVRIALVENEQRIRQAARNIKKFLSS
                     EPQSLHNVIPLAKQA"
     gene            4695..5111
                     /locus_tag="PK264_18535"
     CDS             4695..5111
                     /locus_tag="PK264_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77906.1"
                     /translation="MKALTTTLALATAAGALSFASVTTEPAHAASHYAMVCLSNSTTA
                     NIKFTWKEGNGASRTAFIAPGSNLKWTYRYAHANDNRSPRLVVSYDADARATNFTQHT
                     HLERYATVGDTCGEAKRYAFQYEPANRNFIRIVVLN"
     assembly_gap    5282..5312
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5824..>6110
                     /locus_tag="PK264_18540"
     CDS             5824..>6110
                     /locus_tag="PK264_18540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77907.1"
                     /translation="MQKMNDPRSFDLGPYKINNPDELTRNIVKAWEEGSRLMARFIER
                     SDKSVSASPFSPGGEMMDMSKTFQALWQQWLAEPHKLVEAQGDLMRSYMEL"
     assembly_gap    6111..6127
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6128..7642
                     /gene="phaC"
                     /locus_tag="PK264_18545"
     CDS             <6128..7642
                     /gene="phaC"
                     /locus_tag="PK264_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HRD77908.1"
                     /translation="NDVKPVAVPEAGDGRFRDPEWTTNPFYDFWKQAYLITTQWADAT
                     LERTEGLDEMTRKKAEYYFRQLSSALSPSNFPITNPEVMRETLASNGRNLVAGMQQLA
                     DDMNRSDGPLKISQTDTTAFEVGRNLAVTPGKVIYQNDVLQLIQYTPTTDKVRELPIL
                     IIPPWINKFYILDLTPPKSFIKFLVEQGFTVFVASWVNPDERLAGKTFENYMKEGILA
                     AADAVLRETGAPKTNVLGYCVGGTLLSTTLAYVAERGNDPFASATFLAAQADFSKAGD
                     LKLFTGHEQLKVIEGLMAEKGYLDGSRMATMFNMLRPRDLIWPYIVNNYMLGKKPMPF
                     DLLFWNQDSTRMPAANHNFYLREFYLENKLAKGDLVIDGTRLSLKKVKLPIYELAARE
                     DHIAPAESVYIGAKCYGGPVEYVMAGSGHIAGVVNPPAPEGKKAKYQFWTNSKLPATV
                     DEWLAGATETPGSWWPHWSKWLASHSGSMVAARRPGAKLGTIEDAPGSFIKTKA"
BASE COUNT         1433 a         2476 c         2491 g         1279 t
ORIGIN      
        1 ggacaactct ccgccccgcc ctcctcagtc atgccgcctc ctcacctcac cgccatgccg
       61 gcgaaggccg gcacccggtt cacctcgggg cagcctccac cgttcccacg cgcggtgcat
      121 tgggtcctcg ccttcgcgag gatgacgcgg gtggggcggg cgccgcacgc aaatcgctcg
      181 gcgacagggc ctcgctctga gtttgcgcgc cttacgtgcg cgaggccggc ccagacttgg
      241 ccattcttga ttgcacgcca ccgccgcccg tttcggtttc agggacttca ccggacgcaa
      301 agcaggaggc ggttatctgc atcctcccca gccggcggcg ccggctcaac cgcgggccgg
      361 gacgagtgct tccttgagca cgcgggcgta gttctcgctc gcgaggcggc ggatgcgggc
      421 ctcggggacc ttgcgggtct gccagccgga aaccaccgcg cggaggtcga cgtagctcct
      481 gatcgccgcg ttgccggaaa gcccgttgat gtcggtgccg aaggcgacat ggcggtcgcc
      541 gagccagtcg gcgagctcga gcatgcgatt gccatagccg tcgatcgtct tgccgacatc
      601 gaaggagagc gcccagagcc cgacaacgcc gcccttggcg gcaatcgcct tggcgaggtc
      661 gaggggcagc tggcgcgccc gccagatcac ggcgttccag ggggcggcgg cgcccatctt
      721 cacggaacca tgcgaccaga cgagcggcgc cttggcgaca gcgagcgttt gcgtgaccgt
      781 cggtcccgct gcatgcgcga ggtcgaccag gatgccgagc cggttgcact ccgcgacgac
      841 ctgacggcca gtttcggtca ggccgccgtg caccggcgcc tcggtctgaa agtcgccgag
      901 cgtattcgcc gtgaaatgga cgagctggag atggcggacg ccgaggtcgt aggcgcgctt
      961 cacccgtgag gcatcgccct cgatgaaatt cgcgccttcg atggagagca cgatgtgcgg
     1021 ctcgcccttc tccgcgcgct cgacgtcggc ggcggtcagg gcgagcttca ggccttgttc
     1081 ggctgagtgt gctttgatgc gaccgatctc ccgctcgacc cagccgagcg cctcgccggg
     1141 gcgctgttcc ttcgactggc ggtaggtctt cgggtccatc cagagggcat cgccggcgac
     1201 cgaccaggag acgagtgtcg cgccgccgtc ggcgagcgac ttggccacgg gccgcgagtg
     1261 attgatgctt gcgccgaaga acgagtggaa gtgcatgtcg gaaatgtgga tcggtccggt
     1321 ggcccgggtc gacgacggcg cgagggttcc agcggcgatc gcggtctgga gtaagcggcg
     1381 gcgattcatg gcggcctccc aatgtgggct aggagcaccc tcggcgggtg ttggtggcag
     1441 tccaggcgtc tcaccaaccg gacagcaggg agccccgcgc gcacagctcg acattcagac
     1501 ttcagagcaa gcgattgtcc agcttcagac ggcggactat ggtgaagggg ctgctaccat
     1561 agagactgat ttcaatgcag gaggcacgct tatgcgttcg acatatctga ttgccgccgt
     1621 ggttgcgctc gccattccgc tcgcggcaat gccgtggtcg gagacagatg tccttgcgca
     1681 ggatgacaag gcgaagcgga agaagtccaa ttctaaatcg agcgaccgga aaccgagccc
     1741 gccgcccgcg agcaagagtt cgggtggcta cccgtcgtgc aaccaaggcg tgctctgggt
     1801 cgacacgctg tgccgcctcg atgacggccg ggtctgcctc gtcggcgagt tcgacattac
     1861 ggactgcaag tgatcgcgct gcgccccgtc gcgtctgccg atcagccgag caccagggcc
     1921 gccaacgcaa taccgaccga tgtgagaatg ctcggcatgt cggcgagccc cacgaacgac
     1981 aggatgccgt aggagatcac ggcggacgcc accgtcacga cgacgcggcg cagggtctgc
     2041 cgcgccagcg acgcgggcgc gggagcgccc gttgccgcga atggtcgggc gacacgtgtc
     2101 gccggtgtgc ccggatggcg gcccggcttg aacagccagc cgaggaagcc cgcgagcacg
     2161 tacggcagcg tgatgagcag catgatgtgg gcgggatcca agccgggcct gcggaagtgc
     2221 ctgtaggccc tggcactcgg agcgtcccac atgccggcga tcatgccgga cacgggtatc
     2281 aagatggcga gcacgaacag ataggccaca acgctcttgt cgcgcttgat cttccagccg
     2341 agcaggaacg agacgagcgc gaggccgccg aagacgagat agtcacgttc gagcccaccg
     2401 cccatgtgcg cctcttcaaa ggaacaattc tccgcccccc gcgagggcgg caaatctcat
     2461 tgcatccgcg aacggaatac caccgtgccg cgtttcgagc caccgagcga cacgccggga
     2521 tgccgctcgt tccagccacg cagagacggc atgctcaagg cacaggcagg cgccggccga
     2581 ggcatcgcca gcctcggccg cggccgtcgt cgaggcgtcg acttctgtca ggaaggtcag
     2641 gtggaagcgg ttgaccatgt tggagaggcc gaccgtcagg cagatatcga cgatctgggc
     2701 cggcgagaaa tgccgctcga gtgcgctgta ggtctgagcg tcgatctgct cgagccgcgt
     2761 cgacttctgc gcatagcgca cgatcgccgc ttccgccgcg tcgtagaccc ccgcggggag
     2821 agggtcgtcg atcaggtggc gcagctgcgc ttcagtcaat cccaggctcc gaccgagcac
     2881 tgtatgcgtg ggtattcaat aatggcagtt gttgacggcg gtcgccgtca ggtaggcgag
     2941 ctcgccgtgg cggggcgcga gcgtcgagcg gccgcggtag accgtctcga cgaaattcag
     3001 gaaggcgccg agcgcctccg gccggttcgc catggcgcga tggatgttga gcagcgcgcc
     3061 ccgcgaacgg gcggccgcct cgagcgccgc gcgcgtatcc ctcgagacgg caggatcggc
     3121 cggatcgtgc agcggaattc gggccatggc gcgctccttc agatcatctc aagcctgctt
     3181 ggcgagcggg atcacgttgt gcagcgattg cggctcgctc gacaggaatt tcttgatgtt
     3241 gcgcgccgcc tggcggatgc gctgctcgtt ctcgacgagg gcgatgcgta catagccgtc
     3301 gccgtgctcg ccgaagccga tgccgggcgc gaccgcgacg ctcgccttct cgacgaggag
     3361 cttcgagaac tcgagcgagc cgagggcttc gaaggccttg ggcaccggca cccaagcgaa
     3421 catggaggcg ctcggggccg ggaacggcca gccggcattc gtgaagctct cgaccaggac
     3481 atcgcggcgg cgcttgtagg tcgtccgcat ctcgtcgatg cactcggtcg ggccattgag
     3541 ggctgcggcc gcggccacct ggatcggcgt gtaggcgcca tagtcgaggt agctcttgac
     3601 gcgggcgaga gcggcgagga gccgctcgtt gcccaccgca aaacccatgc gccagccggc
     3661 catcgagaac gtcttcgaca tcgaggtgaa ctcgacggtc acgtccatgg cgcccggcac
     3721 ctgcagcacg gagggcggcg ggttcttctc gtcgaagtac acttcggcgt aggccagatc
     3781 cgaaaggatg atgatgtcgt gcttcttcgc gaattggacg aggtcgcggt agaaatcgat
     3841 cgtggcgacg taggccgtcg ggttcgccgg gtagcagacg acgacggcga tcggcttcgg
     3901 gatcgagtgg atgagagccc gctcgagcgc cgtgaagaac tcgggcgtcg gctcggaagg
     3961 caccgagcgg attacgccgc cggccatcag gaagccgaag gcgtggatcg gatagctcgg
     4021 gttcggcacc agaacgacgt caccgggcgc ggtgatcgcc tgcgccatgt tggcgaagcc
     4081 ttccttcgag ccgagcgtgg cgacgacctg cgactcgggc ttcagcttca cgccgaagcg
     4141 gcggccgtag tagccggcct gcgcgcgcag gagcccagga atgcccttcg aggcggagta
     4201 gcgatcggtc cgcggcttgc cgacggtctc agtcatcttc tcgagaatgt gggccggtac
     4261 cgcgagatcc gggttcccca tgccgaggtc gatgatgtcc gcacctcgcg ctctggcctc
     4321 ggccttgagc cgattgacct cggcaaagac gtagggcggc aggcgcttga tacggtggaa
     4381 ttcttggacc acggatgacc tcggtgatgg agtgtgatgc aggcgccgag cccggcatca
     4441 ggcggaaact ctgtaaggcc agtacgccat cgccgtcaaa gcccgtcccg atggtcgggt
     4501 agccactcga tccgacggcc cccattcgga tcggcctgca gaagcaagcc atttcgtgaa
     4561 ccttgcatga acaaggaaat agttcacgtt catgaccaaa acatgaagaa ataatttctg
     4621 tccggcccaa ttcgatgacg tgccgaaagg cgcaatacag tcagtcgaac caagtaagtt
     4681 ccgggagaat ttccatgaaa gcccttacta cgacgcttgc tcttgccacc gcagccggtg
     4741 cgctttcgtt tgccagcgtg accaccgagc cggcccacgc tgccagccac tacgcgatgg
     4801 tctgcctcag caacagcacc acggcaaaca taaagttcac ttggaaggaa ggcaacggcg
     4861 cgtccagaac tgccttcatc gcgccgggca gcaacctgaa gtggacatac cgctacgccc
     4921 acgccaacga caaccgctcg ccgagactag tcgtcagtta tgacgccgac gcccgcgcca
     4981 cgaacttcac gcagcacacg cacctcgagc gatatgcgac cgtcggtgat acgtgcggcg
     5041 aggcaaagcg ctacgccttc cagtacgagc cggcgaatcg caacttcatc aggatcgtgg
     5101 tgcttaacta acagcacccc aacgccctga catatcgcac cccggaggca accccggggt
     5161 gcttgcgttg tgctgtcggg cagcgcagct attgcttcgc ggccgttttc tcggcttcaa
     5221 tgcgggcagc ctctttgcgt ttgttgagct cgtcgatggc agccgcctgc tgctccttcg
     5281 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcagctc gaccgtcgtc atggtctcgc
     5341 cgcaggcgcc ggccacggcg ctcgccagaa gcgcgagcgc caccagtccg agccgcgccc
     5401 gcccgagcgt ccggctgacc gggatgggca gaggtctcat gtccgggtcc gtcatggcgt
     5461 atgctcgacg aatcatgttg ctgcaatgca gcatagccta aagtgaatgg tcttgacaga
     5521 ttgtcagtcc gcgcggttgc ggttagaagc acgacctaaa gtaccgaggt tcgcaagctg
     5581 accactcgag tgcgccgcca ggagagcgta tcattaggat tgtgtcgatt ttcccggcac
     5641 gcaccatccg gtcaggatgc gagacttgga gcgacatgca acttggcggc atgcaacgtc
     5701 tgaccaaact cgtccgcggt caacccgacg tgtcgtccat gatccaaatt ctcgagtgcg
     5761 agcgccgaga ctgcgaccga cggcggcgcg cttgctgtgc agcgcccaat tcacgagcga
     5821 gccatgcaga agatgaatga tccccgtagc ttcgatcttg gaccctacaa gatcaacaat
     5881 cccgacgagc tgacgcgcaa catcgtcaaa gcatgggagg aggggagccg tctcatggcg
     5941 cgcttcatcg agcgctccga caagagtgtc agcgcaagcc cgttcagccc gggcggcgag
     6001 atgatggaca tgtcgaagac gtttcaggcc ctctggcagc agtggctcgc cgagccgcac
     6061 aagctcgtcg aggcgcaggg cgacctcatg cgcagctaca tggaactctg nnnnnnnnnn
     6121 nnnnnnnaac gatgtgaagc cggtcgccgt tcccgaggca ggcgacgggc gcttccgcga
     6181 ccccgagtgg acgaccaacc cattctacga cttctggaag caggcctacc tcatcaccac
     6241 gcagtgggcc gacgccacgc tcgagcgcac cgagggcctc gacgaaatga cgcgcaagaa
     6301 ggcggaatac tatttccgcc agctctcgag cgcactctcg ccgtccaact tcccgatcac
     6361 caaccccgag gtgatgcgcg agacactcgc cagcaacggc cgcaatctcg tcgccggcat
     6421 gcagcagctc gccgacgaca tgaaccgctc cgacggtccg ctgaaaatca gccagaccga
     6481 tacgactgcc tttgaggtcg gccgcaacct ggcggtcacg cccggcaagg taatctacca
     6541 gaacgacgtg ctgcagctga tccagtacac gccgaccacc gacaaggtgc gcgagctgcc
     6601 gatcctcatc atcccgccgt ggatcaacaa gttctacatt ctcgatctca caccgccgaa
     6661 gtccttcatc aagttcctgg tggagcaggg cttcacggtg ttcgtcgcga gctgggtcaa
     6721 tccggacgag cgcctcgccg gcaagacctt tgagaactac atgaaagagg gcattctcgc
     6781 cgcggccgac gccgtgctgc gcgagacggg tgcgcccaag accaacgtgc tcggttactg
     6841 cgtcggcggc accttgctct cgactacgct cgcctacgtc gctgagcgcg gcaacgatcc
     6901 gttcgcttcg gcgacattcc tcgcggcgca ggctgatttc tcgaaggccg gcgacctcaa
     6961 gctcttcacc gggcacgagc agctcaaggt gatcgagggc ctgatggccg agaagggcta
     7021 tctcgacggc tcgcgcatgg cgacgatgtt caatatgttg cgcccgcgcg atctcatctg
     7081 gccctacatc gtcaacaact acatgctcgg caagaagccg atgccgttcg acctgctgtt
     7141 ctggaaccag gattcgacgc gcatgccggc cgccaaccac aacttctacc tgcgcgagtt
     7201 ctacctcgag aacaagctcg ccaagggcga cctcgtcata gacggcacac gcctcagcct
     7261 gaagaaggtg aagctgccga tctatgagct cgccgcccgc gaggaccaca tcgcgccggc
     7321 cgagtcggtc tacatcggtg ccaaatgcta cggtggaccg gtcgagtatg tcatggcggg
     7381 ctcgggacac atcgccggtg tcgtcaatcc gccggccccc gagggcaaga aggccaagta
     7441 tcagttctgg accaacagca agctgccggc gaccgtcgac gagtggctgg caggcgccac
     7501 ggagacgccc ggatcctggt ggccccactg gtcgaagtgg ctcgcctcgc attccggaag
     7561 catggtcgcg gcgagaaggc ccggcgccaa gctcggcacc attgaggatg cgcccggctc
     7621 cttcatcaag accaaggcct gacgatcacc tgcatgggcg tcggcacgag gagccctggg
     7681 ccccgctcga tcttacagtt ttgcacacga cgcgcggcgc ggacctt
//
LOCUS       DAORNH010000220         7712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7008_length_7712_cov_3.472509, whole genome shotgun sequence.
ACCESSION   DAORNH010000220 DAORNH010000000
VERSION     DAORNH010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_7712_cov_3.472509"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>270
                     /locus_tag="PK264_18550"
     CDS             <1..>270
                     /locus_tag="PK264_18550"
                     /inference="COORDINATES: protein motif:HMM:NF015487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HRD77909.1"
                     /translation="VLAEANTAARQRPTPDAFNQARHIFSYEPGALYELYTNPNYVST
                     ILLEPGETLNDIAAGDTSRWMVTEATAETESEPRTIVLVKPQTTG"
     assembly_gap    271..280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <281..710
                     /locus_tag="PK264_18555"
     CDS             <281..710
                     /locus_tag="PK264_18555"
                     /inference="COORDINATES: protein motif:HMM:NF015487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HRD77910.1"
                     /translation="NVVLITDRRTYLIEAVSQAGNAYAAQVAWSYPQNAAAQTGAIPS
                     IDRLNFSYRVRTVRGRTPIWTPARVFDDGRRTWIEFPPGVAATDMPPLFVITGEGAEI
                     ANYRVQGQRMIVDRLFDRAELRLGVRAPIIVRIDREGVRR"
     gene            707..1771
                     /locus_tag="PK264_18560"
     CDS             707..1771
                     /locus_tag="PK264_18560"
                     /inference="COORDINATES: protein motif:HMM:NF015688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HRD77911.1"
                     /translation="MNTATTSEAPNLALRAQPPSPKRLSRKVIFAGLILAGIVIAFAL
                     VSGLSERPAGRGDTAEAAVAAPGGPPESIQQASADYDARALIGGRELEIDPFDAGDGD
                     ELAPPNDPLWAGRADTTSAPAGAALPPNPQDVARTSPILFAGRDASVGAATRTDADRL
                     DTRLSPPRSRYELQAGHVIPAALVTELVSDLPGRVIAQVTAPVYDSVTGDHLLIPQGS
                     RLIGTYRDGARYGDNRILLAWNRLILPNGWSINLQNMDGSDPAGASGLRDRTDNHLDR
                     LAAAIALSSIITVGANEVEDNESNGIMSSLGDAAAQQAAQVGSRIVDRELSVRPTLRV
                     RAGAPVRVVIGRDIQLRPYR"
     gene            1780..2256
                     /locus_tag="PK264_18565"
     CDS             1780..2256
                     /locus_tag="PK264_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77912.1"
                     /translation="MASPTKGPRSRTSDGPDLFEFAATQETTALVAAPALKRAPRPAP
                     VSKKAVRPLPAGRMEAAVSTVSVKAMPTYPDELHRAVERSLAELPSDKVWFTYRDIKR
                     HFGVSRATVARRVKDGLVPGIRRQRDRVLDDGAVRRFDRVQLHWLLLAVRFARNSE"
     gene            complement(2263..4776)
                     /locus_tag="PK264_18570"
     CDS             complement(2263..4776)
                     /locus_tag="PK264_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRD77913.1"
                     /translation="MANPLQIVLNPEHYQQARDVPDGGGPPHDFFADRDKAFAAHKTQ
                     LERQANALATSIAAQPEGPIGVIKVILRRGAWAKSHRPIRSLLRLDRWTLVGGGSLGE
                     MHFEATPSLLRELGQRVTKAEPETRWRDVGDPPKPTPNPTRLRSEVGAIERLELYGPD
                     DRRKFSAADAVSWLSNSRTGGHYLVDLFEPIPPEERWDATSPARRQLFTTFREGLRGL
                     GLGVRTIAVAGRGGDAARFLLRLERSNQPALIPQLTRAEVRSRDIVPFDTTVARHEQL
                     LAFLDKHPLVRRVRLPGVLSSRARRPAAPPSPTRARPIALALPTKNDALNYPIVGVID
                     GGVTDTLHPWVVGRHGLLADEHMDLAHGSFISGIIVNGNALNGPEVSRDPDGVNIVDI
                     NVFPDENHPTAFSDYYPNGLTDFFNEVESAVANAQARWGTRIFNFSLNIPEPVAEDAY
                     SVYAARLDEIAEAHNVIFVISAGNLRENPRPEWPETEAGALAAIAAGRNDGIEMPAES
                     ARNIAVGALNPPDLSSCIAYAPACYTRRGPGLRAGAKPDVACVGGGGTLCSVLDEGLF
                     SIDPTGATVAGCGTSYAAPWAAKCLAHLDAAIEGEVSRETLHALLIHHSEAPKVFAPK
                     QLQPFARHLVGFGMPISALEMLDRSPHEATIVIATRLPPDQQIVLPFSWPQSLTAPPG
                     KCRGSASLTLVSSAPIDRRYGAEFMRVNVDAALQQAQLDGGWKGRAAPIYLPGVPDGH
                     AYEADLVEHGLKWNPVKRYAMHSPRGAGKSTSWRLLIKYTTRTAEAVPPEGIPFTAIL
                     TIRDPAGAAPVFQEVQQGLTTTGVQIADIRTAVRVKPRI"
     gene            complement(4776..5972)
                     /locus_tag="PK264_18575"
     CDS             complement(4776..5972)
                     /locus_tag="PK264_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019087229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77914.1"
                     /translation="MDHFEIIQALCRSGLPNGGTVFRRQVERLRDALAAAGDPKAAAS
                     IGGLLKAAERTKEVAPSRLTASRRPFGGETLTPAAPPPVDRETGAALASIIFPNDIAD
                     EAPLFTSSITAALESLIEEWRHVQQLEEINVRPAKSCLIYGPPGAGKTRLAYWIAKRT
                     GLPIISARIDGLVSSFLGTTARNIAALFTFANRYQAILLLDEFDAVAKVRDDPHEVGE
                     IKRVVNTLLQNLDMRENVGVTIGITNHSALLDPAVWRRFDVQLEVPRPDFETRLAIAG
                     RYMQPMSVPDSHLRFIAWLTDHATGAEVEASVRAYKKSHAVNKNIRAVADIFRQFVVL
                     NAGRITDERRQLLEGPDAQLEAALQRDPDLSFSTRDAGEVTGVNKSTVSRRSGRAGAA
                     RKKSVG"
     gene            complement(6186..7427)
                     /locus_tag="PK264_18580"
     CDS             complement(6186..7427)
                     /locus_tag="PK264_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HRD77915.1"
                     /translation="MKLSDLKCRKAVATERPIKLADGGGLYLHVYPNGRKLWRLAYRW
                     GGKQRIMPFGPYPHITLLEARKKREAAKDELREGIDPMAARRAEDAAQEALGETTFAA
                     FAGRYLERLKMAGRSEPTIKKAKWIVEDLAKDLRPQQVADIKPRDVLKVLRVIEAKGN
                     RETARRMRGILSAVFRLAVIEDVIDADPSAPLKGALLPPETRHNSAVTEPEAFGGLLR
                     AIDGYDGHKTIKIALQLLALTFPRPGELRQAEWPEVDIPGKLWIIPAPRTKMRREHRI
                     PLSRQAADLFEALKEITGGGTRCFPSIRSLDNPLSDGALNAALRAMGIDGAVHVAHGF
                     RSSASSLLNEHSAFSPEVIERALAHGEPDKVRRAYNRAQYWDERVRMMQWWADYLDRL
                     KAKGVRSIPAKADDVQPELFS"
BASE COUNT         1386 a         2425 c         2487 g         1404 t
ORIGIN      
        1 agtgctggcg gaggcaaaca ccgccgcgcg tcagcggcca acgccggacg ccttcaacca
       61 ggcgcggcac atcttcagct atgagccggg tgcgctctac gaactttaca ccaaccccaa
      121 ttacgtctcg accattctgc tcgaacccgg cgagacgctc aatgacatcg ccgcgggcga
      181 tacctctcgc tggatggtga cagaagcgac cgccgaaacg gagagcgagc cgcgcacgat
      241 tgtcttggtg aagccgcaga cgaccggctt nnnnnnnnnn caatgttgtg ctgatcacgg
      301 atcggcgaac ctatctcatt gaggccgtgt ctcaggcggg caatgcctat gccgcgcaag
      361 tggcgtggtc ctacccgcag aacgccgcgg cgcagacagg cgccatccca agcatcgatc
      421 ggctgaactt ttcctatcgc gtccggaccg tgcgggggcg aacgcccatc tggacgccgg
      481 cccgcgtgtt cgacgacggg cgcaggacat ggatagagtt tccgccgggc gtcgcggcga
      541 cggacatgcc gccgctcttt gtcatcaccg gcgaaggcgc ggaaatcgcg aactatcgtg
      601 tgcaagggca gcgcatgatc gtcgatcgcc tgttcgatcg cgccgaactc cggcttggcg
      661 tccgtgcccc catcatcgtc cgcatcgacc gtgaaggagt ccggcgatga ataccgccac
      721 gacatctgag gcgcccaatt tggcgctacg cgctcaaccg ccatcgccaa agcggctttc
      781 gcgcaaagtc atcttcgccg gccttattct ggcgggcatc gtgatcgcgt ttgcactcgt
      841 cagcggtctc tcggagcgtc ctgccggacg cggtgacact gccgaagctg ccgtcgccgc
      901 gcccggcggg ccgccagagt cgattcagca ggcttctgcg gactatgacg cgcgggcgct
      961 gatcggcgga cgagaattgg agatcgatcc gtttgatgcc ggcgatggcg acgaactggc
     1021 gccgccgaac gatcccctct gggcaggtag ggcggataca acgagcgctc ctgccggtgc
     1081 agcgctcccg ccaaacccgc aagacgtagc acgaacctca ccgatcttgt tcgcggggcg
     1141 ggatgcgtcc gtcggcgctg cgacgcgtac ggacgcggat aggcttgata cgcgcctgtc
     1201 gccgccgcgc tcgcgctatg agctgcaagc tgggcatgtc atcccagcgg cgcttgttac
     1261 cgaacttgtc tcagatttgc cgggccgcgt gatcgcgcaa gtaacagcgc cagtctatga
     1321 cagcgtgaca ggcgatcatc tgctcatccc gcagggttct cgcctcatcg gcacatatcg
     1381 cgacggtgcg cgctatggcg acaaccgcat cttgcttgcc tggaaccggc tgatcctgcc
     1441 gaacggctgg agcataaact tgcagaacat ggatgggagc gatccggccg gcgcttcggg
     1501 attgagagat cgaacggata accatctcga ccggctcgcg gcggcgatcg cgctctcgtc
     1561 gatcatcacc gttggcgcca acgaagtcga agacaacgag tcgaacggca tcatgtcaag
     1621 cctcggcgac gccgcggcgc aacaggcggc gcaagtcggc tctcgcatcg tcgatcgcga
     1681 actctcggtg cgtccgacat tgcgcgttcg cgccggcgcg ccggtgcgtg tggtgatcgg
     1741 tcgagacatt caactacgac cataccgata ggagcggtca tggcgtcgcc cacaaaagga
     1801 ccgcgatcgc gaacgtcgga cgggcccgac ctgttcgagt tcgcggcgac gcaggaaacg
     1861 acagcgttgg tcgccgcgcc ggcgctcaag cgagcgccca ggcccgcgcc ggtgtctaag
     1921 aaagcagttc gaccgcttcc cgcaggcagg atggaggcgg cggtctccac cgtctccgtg
     1981 aaggcgatgc caacttatcc tgacgaactc catcgggccg tggagcgctc attggccgag
     2041 cttccatcgg acaaagtttg gttcacttac cgagacatca agaggcattt cggcgtatcg
     2101 cgcgcaacgg tggcgcgtcg cgtaaaggat gggcttgttc ccggcattcg ccggcaacgt
     2161 gatcgcgtgc tcgatgacgg cgccgttcgt cgttttgatc gcgtgcaatt gcactggctc
     2221 ttgctcgccg ttcgcttcgc gcgaaattca gaatagcgga ggctaaatcc ttggcttgac
     2281 gcgaaccgct gtgcggatgt ccgcgatctg cacgcccgtc gtggtgagac cttgctgcac
     2341 ctcttgaaac acaggcgcgg cgccagcggg atcgcggatc gtgaggatgg ccgtaaacgg
     2401 aatgccctcc ggcggcacgg cttccgccgt ccgcgtggtg tacttgatga gcaaacgcca
     2461 gctcgtcgac ttgcccgctc cgcgagggct atgcatcgca tagcgcttca ccggattcca
     2521 tttcaggccg tgctcgacca gatcggcctc gtaggcgtga ccgtccggaa caccaggaag
     2581 gtagatcggc gcggcccgcc ccttccagcc tccatcaagc tgggcttgct gcaacgctgc
     2641 atcgacgttc acgcgcatga attccgcgcc ataacgccga tcaatcggcg ctgacgatac
     2701 cagcgtcaag ctggctgagc cacgacactt gccgggcggc gccgtcaacg actgcggcca
     2761 ggagaacggc agaacaatct gctgatcggg cggcaagcgc gtggcgatga cgatcgtcgc
     2821 ttcgtgcggc gagcgatcaa gcatctccaa tgcggatatc ggcatgccga aacccaccaa
     2881 gtgccgcgca aagggctgaa gctgcttggg cgcgaacacc ttcggcgctt cggaatgatg
     2941 gatcagaagc gcatgaagcg tttctcgcga tacctctcct tcaatggcgg cgtcgagatg
     3001 cgccaagcac ttcgccgccc acggggcagc gtagcttgtg ccgcaaccag cgaccgtcgc
     3061 gccggtcggg tcgatggaga agagaccttc atccaggacg ctgcaaagcg tgccgccgcc
     3121 gccgacgcag gcaacgtccg gctttgcgcc cgcgcgcaag cctgggccgc gtcgcgtgta
     3181 gcacgctggc gcgtaggcga tgcacgaaga caggtcgggt gggttaagcg cgccgaccgc
     3241 gatgttgcgg gcgctctcgg cgggcatttc gatgccgtcg ttgcgcccgg cggcgatggc
     3301 cgcgagcgca ccagcttcgg tctccggcca ttccggacgc gggttctctc gcaaattgcc
     3361 ggcggagatc acgaagatga cgttgtgcgc ctcggcgatt tcgtccagcc gcgcggcata
     3421 gacgctgtag gcgtcctcgg ccaccggctc cggaatgttg agactgaagt tgaaaatccg
     3481 cgtgccccaa cgcgcctgcg cgttcgccac cgcgctctca acttcgttga agaagtcggt
     3541 gaggccgttg ggataatagt cggagaacgc ggtcgggtga ttttcgtcgg gaaacacatt
     3601 gatatcgacg atgttcacgc cgtccggatc gcgagacacc tccggcccat tcagcgcgtt
     3661 gccgttgacg ataatgccgc tgatgaagct gccatgtgcg agatccatgt gttcgtcggc
     3721 gagcaggccg tggcgcccga ccacccacgg atgcagggtg tcggtgacgc cgccgtcgat
     3781 gacgccgacg atgggataat tcaaagcgtc gttcttggtt ggcagcgcaa gcgcgattgg
     3841 ccgcgcccgc gtcggcgacg gcggagctgc gggcctgcgg gcgcgcgaag agagcacgcc
     3901 tggcaatcgc acgcgccgca ccagcggatg cttgtcgagg aacgccagca attgctcgtg
     3961 gcgcgcgacc gttgtgtcga aggggacgat gtcccgcgac cgcacctcgg cgcgggtcaa
     4021 ttgcgggatc aatgcaggct ggttggatcg ctccaagcgc agaagaaagc gcgcagcgtc
     4081 gccgccgcgg ccggcgacgg cgatggtgcg cacgcccaat cccaagccgc gcaagccctc
     4141 acgaaaggtc gtgaatagtt ggcgccgcgc tggcgaggtc gcgtcccagc gctcttctgg
     4201 agggatcggc tcgaacagat cgacaaggta gtgtccgccc gtgcgcgaat tggagagcca
     4261 cgacacagcg tcggccgcgg aaaacttgcg gcgatcatcg ggcccgtaga gttcaagccg
     4321 ctcgatggcg ccgacctcgc tacgcaggcg cgttgggttc ggcgtgggtt tcggaggatc
     4381 gccaacgtcg cgccaacgcg tttcgggctc ggctttggtg acgcgctggc cgagttcgcg
     4441 cagcaagctc ggcgtggctt caaagtgcat ctcaccgaga gaaccgccgc cgaccaaagt
     4501 ccagcgatcg agacggagga gcgatcggat cgggcgatga gacttggccc atgcaccgcg
     4561 ccgcaggatg accttgatga cgccgatcgg cccctccggc tgcgccgcga tcgaagtggc
     4621 gagcgcattg gcctgacgtt caagctgcgt cttgtgcgcg gcgaaggctt tgtcgcgatc
     4681 agcgaagaaa tcgtgtggcg gaccgccgcc gtcaggtacg tcgcgcgcct gctggtagtg
     4741 ctccggattg aggacaattt ggagggggtt cgccatcagc cgacactttt cttgcgtgcg
     4801 gcgccggcac ggccgctgcg gcggctcacc gtgctcttgt tgacgccagt tacttcaccc
     4861 gcgtcccgcg tggagaagct gagatcaggg tcgcgctgaa gggctgcctc aagctgggcg
     4921 tccggacctt ccagcaattg acgccgctca tcggttatcc gtcctgcgtt gagcacgacg
     4981 aactgacgaa agatgtccgc cacagcgcgg atgtttttgt tcactgcgtg cgacttctta
     5041 tacgcgcgaa ctgacgcctc cacttctgcg ccggtagcgt gatccgtgag ccaagcgatg
     5101 aacctgagat ggctatccgg gaccgacatc ggctgcatgt atcgccctgc aatcgcgagg
     5161 cgcgtctcga aatcgggacg cggcacttca agctgaacgt caaaacgacg ccaaacggca
     5221 gggtcgagca gcgcggagtg attggtgatg ccgatcgtga cgccgacatt ctcgcgcatg
     5281 tcgaggttct gcagcagcgt gttcacgacg cgcttgatct cgccgacctc gtgcggatcg
     5341 tcgcgcactt tcgcgacggc gtcgaactca tcgagcaaca ggatcgcctg atacctgttc
     5401 gcgaacgtga agagcgctgc gatattgcgc gccgtcgtgc cgaggaatga cgacaccagt
     5461 ccgtcgatgc gcgctgagat gatcggaaga ccggtgcgct tggcgatcca atacgcaagg
     5521 cgcgtctttc ccgcgccggg cgggccatag atcaggcaag actttgccgg ccgaacattg
     5581 atctcttcga gttgttggac gtgacgccat tcttcgatca gcgactccag cgccgccgtg
     5641 atgctcgacg tgaaaagcgg cgcctcgtcg gcgatgtcat tggggaagat gatcgacgcc
     5701 agcgccgcgc cggtttcccg gtcaaccggc ggcggggccg cgggcgtcag cgtctccccg
     5761 ccgaagggcc gtcgcgaagc cgtcaggcgg ctcggcgcga cctccttcgt ccgctcggcg
     5821 gctttcagca aaccgccgat ggacgcggcg gccttcggat cgccggctgc ggccagcgca
     5881 tcgcgcaacc gctccacctg acggcggaat accgtccccc cgttggggag gccggatcgg
     5941 cagagcgcct gtatgatctc aaagtggtcc acgacggctc ccaggtgcaa cgttccgtat
     6001 ttgtatcaca acaatcgttg caagtaaacg ttccgacgtt gcgtaaaatg gcgctatcgt
     6061 tgcgcacgac gcgccgaaaa ggccgattgc cgttaatatt tcgttgacag cgcgccgccg
     6121 cgggtggcca cgaccgccgc ccccgcgcct ggattggccc ccgaggcggc ttctgagccc
     6181 cccggttacg agaagagttc tggctgcacg tcgtcggctt tggccggtat tgatctgacg
     6241 cctttggcct tgagccggtc cagatagtcc gcccaccact gcatcatgcg cacgcgctca
     6301 tcccaatact gcgcgcggtt gtaggcgcgt cggaccttat ccggctcgcc gtgcgccaga
     6361 gcgcgctcga tcacttcggg cgaaaatgct gagtgctcgt tgagcagtga cgaggctgag
     6421 cttcggaagc cgtgcgcgac atgcacggcg ccgtcgatgc ccattgcgcg aagcgctgca
     6481 ttgagcgcgc catcgctcag aggattgtcg agcgagcgga tggagggaaa gcagcgcgtt
     6541 ccgccgccgg tgatttcctt gagcgcttcg aacaggtccg ctgcttgccg cgatagggga
     6601 atgcggtgct cacgccgcat ctttgtccgc ggcgcgggaa tgatccagag cttgcccgga
     6661 atatcgactt ccggccattc agcttggcgc aattcgcctg gacgcgggaa cgtcagcgcc
     6721 aggagctgga gcgcgatctt gatggtcttg tggccgtcat agccgtcgat tgcgcgtaac
     6781 agaccgccga acgcttcggg ctccgtgaca gcggaattgt gtctggtctc cggtggaagc
     6841 agcgcgccct tcaagggcgc cgaaggatcg gcgtcgataa cgtcctcgat cacggcgagc
     6901 cgaaagacgg ccgagaggat gccgcgcatc cggcgagcgg tttcgcgatt gcccttggcc
     6961 tcgatgacgc gcagcacttt cagcacgtcg cgcggcttga tgtcggcgac ttgctgcggg
     7021 cggaggtctt tcgccaagtc ctcgacgatc catttcgcct tcttgatcgt cggctccgac
     7081 cggcctgcca tcttcaagcg ttcgaggtag cgcccggcga aggcggcgaa cgtcgtctcg
     7141 cccagtgcct cctgcgccgc atcttcggcg cggcgcgcgg ccatcgggtc tatcccctca
     7201 cgcaactcgt ccttcgcggc ttcgcgcttc ttgcgcgctt cgagtaacgt gatgtgcgga
     7261 tacggaccga acggcatgat ccgttgcttg ccgccccatc gataggccaa gcgccaaagc
     7321 ttccgtccat tcggatagac gtgaaggtaa agcccgccgc cgtcggcgag cttgatgggc
     7381 cgctccgtgg caaccgcttt tcggcatttg aggtcggaca gtttcatgtt ggcaaatgag
     7441 tcacgcgttg tgttggcaaa tcgccggagg accggaattg ccaacagcat ttgccaacat
     7501 cgcggtgaaa ttcaatgaga cgcgcccggt cggcttgcga cgaccgagtc gaacgtcact
     7561 ggcgcgaaaa agcctttcaa atcaaaatct ttgagacgat ttgagacggc ctgagacggt
     7621 gtggataagt ggccggaaaa tgggtgatgg tgccgcctag gtgactcgaa cacctgacct
     7681 acgcattacg aatgcgccgc tctaccggct ga
//
LOCUS       DAORNH010000221         7666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7059_length_7666_cov_2.672054, whole genome shotgun sequence.
ACCESSION   DAORNH010000221 DAORNH010000000
VERSION     DAORNH010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7059_length_7666_cov_2.672054"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="PK264_18585"
     CDS             <1..1404
                     /locus_tag="PK264_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77916.1"
                     /translation="GVVFGLAPPAEDLPEGEEPKAFTRMIAPPKSSDRRTISERREPG
                     RTSSVGGEPSRARRAGKAPEKKTVVAFSFERVAKLGGLDVPEAKARDTLAALGFGVEG
                     KGKSVKVTAPSWRPDVHGSADIVEEVVRIAGLDTIPSAALPKLHGVTRSVLTEKQRRT
                     RRARRLLASRGMVEAITWSFIERKDAALFGGGSDALELANPISSDMSSMRPTLLVGLL
                     TGAQRNRNRGFSDVALFEIGQVFRGDRPEDQGQVAAGVRAGAAGIGGAGRDWLSPAKD
                     AGLFDAKADAAALLSVLGVDVGKVQITRDAPGWYHPGRSGVIRLGPKTVLAQFGELHP
                     DTLAVLDVAAPAVAFEGFLDAVPAARRKGTQRPPLDVSDLQPVRRDFAFILGSDVAAA
                     DVIRAASGADKKLITAVSVFDQFEGASLGAGKKSLAIEVVIQPRDEPLKDEAIDAIAK
                     KVIAEVKRVTGGEVRGG"
     gene            complement(1424..2662)
                     /locus_tag="PK264_18590"
     CDS             complement(1424..2662)
                     /locus_tag="PK264_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRD77917.1"
                     /translation="MLLSKLLPCLTILAVLLGARPAAAQSYSGDILAFKINELSSILM
                     LQETPGASPPRIVILNPLRHPKDEVLKPVEAKEILTRIEPHLAMVPRLIIRNPEDMGF
                     ALDTLWGHVQKQGHEAARYWDVQQIHLIMWLEGHKEIATGARKFRLVLMNPRTHQKLA
                     SDLYDVIYVAPPPRVEAPLQPSPTLGSSLAKPMPPPRAPPPAVAPPVVATPVPAPVVA
                     SPAPQPTPPKAPPPPAAAKPAPTVADVENIRTLQRELRNRGCFSGDTTGVMDAKTIKA
                     LTDFNKVAAQPVDVHGATLPAIARMKRQPTVRCGVVAPKSTGLMLSKSSSREDILDLE
                     DELRARGCPVGSANGAWTIILDSKVKGLQQDGGLPLSGTVSAGEVLSKLKAAPSIRCV
                     PGCTAEMNKLFPLSCSRIVR"
     gene            complement(2771..3541)
                     /locus_tag="PK264_18595"
     CDS             complement(2771..3541)
                     /locus_tag="PK264_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HRD77918.1"
                     /translation="MLRAKGEFHATLALRSGSTIDIGPGCCYPAGMRHPIPDPSSCLA
                     SGPVVDSDAFEVRQFAARALSCVGSESSEARTIALYYAVRDGLRYDPYSFSVDPVTFK
                     ASHVAAQTVSWCVPKAILLTAALRAADIPAAVGFADVRNHLTTPKLRERMGTDSFINH
                     GYTAVWLHERWIKITPAFNAALCERFGVHPLEFDGKSDALFHEFDRENRRHMEYVADH
                     GFVAEPDLDALIAEYRAAYPRLVAVAAGAASKDEAFQG"
     gene            3583..>4268
                     /locus_tag="PK264_18600"
     CDS             3583..>4268
                     /locus_tag="PK264_18600"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD77919.1"
                     /translation="MSIAHWMRFGFRPAMVAVLLAASAATAIAQVPATDSSMIDGLLG
                     RSRTRSATAVAPQPSAEDKALIDALKSRRTRGVTVEERKAIAVLSDSLPSIDVEIRFD
                     YNSAVITAAAEPALASLGRALNDPRLAGLSFLVCGHTDAVGGAEFNLGLSQRRAEAVQ
                     RYLAERHAVAADRLATVGHGFEQLKDKANPKAAANRRVQIVNLGGTRSVAAAPRPKVV
                     PVSGPPGAKAP"
     assembly_gap    4269..4278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4279..4818
                     /locus_tag="PK264_18605"
     CDS             <4279..4818
                     /locus_tag="PK264_18605"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HRD77920.1"
                     /translation="AKTRSLMDKSLADIAGVTEVSAAPQPPAVTSAPAPLVVKLSPGG
                     ALRVGDLMTVSITSTIAGNLIVYEQDAAGQTTQIFPNNFSDAPQTTIDAGQTITIPGP
                     TSRFQLRVTPPVGASRIIAVVVPARVTVADITRDGADMKPLANPVEVIGKIAERVTRG
                     VRVEPAERAVGIAEYTIAN"
     gene            complement(4837..5412)
                     /locus_tag="PK264_18610"
     CDS             complement(4837..5412)
                     /locus_tag="PK264_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77921.1"
                     /translation="MASRGAIVHLIGPAGVGKLTVARALAPMLDAIVVDNHWINNPVL
                     GLLRHDRVTPYPAQVWTEIEKVREAVLATIAVICPPEQSYILTNELYDGEDDRALGQQ
                     VARAAEMRGSPYLPVRLHCAPEALASRVVSPDRAPRLKSMDADTARRNASRGALMTGS
                     ASELTLDTTDLEPHATALRIAAHVQRVRQAC"
     gene            complement(5496..>7366)
                     /locus_tag="PK264_18615"
     CDS             complement(5496..>7366)
                     /locus_tag="PK264_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRD77922.1"
                     /translation="PPRLNLEDYRPGLDAYDPIPAKPSELRPWTPPIAAGPDSEPAPS
                     MLPVIGHPPDPASAGPAAAIDPRFEETTDVESGAIARRFAPARSDAGHAVSDRPYPPR
                     HVGTESAHQGHGYRRPSLNELKKPVLHRPDAATAEQALRGQARLLLDALADFSVKGEI
                     KDIRPGPVVTVFELEPARGTKASRVIGLADDIARAMSVASVRIAVVPGRSVIGIEMPN
                     ARREAVAMREILESREWRESPATLPIALGKSIAGDPVVMDLARMPHLLVAGTTGSGKS
                     VGVNSMILSLLYRHGPEACRFLMIDPKMLELSVYNGIPHLLTPVVTDPSKAAAALGWV
                     VREMEERYKRMASLAVRNLEMYNARVRNAAKAGEPLSRQVQTGYDPQTGEPVYETETI
                     EHAVMTHIVVVVDEFADLMMVAGKDVEAAVQRLAQMARAAGIHLIMATQRPSVDVITG
                     TIKANFPTRISFKVASKIDSRTILNEMGAEALLGMGDMLVSSGGSGALRVHGAFVADE
                     EVERIAAYLREQGTPDYVLGITEGPEDAPESGDDRGTAAVRAGGDDLYQRAVALVLAD
                     QKASTSYVQRRLGIGYNRAADLIERMEREGLIGPVGVGGKRAILGPSHQTPTGRAA"
     assembly_gap    7367..7456
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7457..>7666)
                     /locus_tag="PK264_18620"
     CDS             complement(<7457..>7666)
                     /locus_tag="PK264_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77923.1"
                     /translation="RPRLLLWPLVLLATAGALSALPILTRWPLVYGHGGMIGDLSFKT
                     VSGLFALINPGRAGVAAGLMLFAAAA"
BASE COUNT         1214 a         2437 c         2670 g         1245 t
ORIGIN      
        1 ggcgtggtgt tcgggctggc gccgccggcc gaggacctgc ccgagggcga ggagcccaag
       61 gcgttcaccc gcatgatcgc gccgccgaaa tcctcggaca gacggacgat ctcggagcgg
      121 cgcgagcccg gcagaaccag cagcgtcggc ggcgagccct cgcgcgcgcg acgggccggc
      181 aaggcgccgg agaagaagac cgtcgtcgcc ttctcgttcg agcgtgtcgc caagctcggc
      241 gggctcgacg tgccggaggc gaaggcccgc gacacactcg ccgcactcgg cttcggcgtc
      301 gagggcaagg gcaagtccgt caaggtgacg gcgccgagct ggcggccgga cgtccacggc
      361 tcggccgata tcgtcgagga ggtcgtgcgg atcgccggcc tcgatacgat cccatcggcc
      421 gccttgccga aactccacgg cgtcacccgc agcgtactga ccgagaagca gcgccggacc
      481 cgccgcgcgc gcaggctgct cgcctcgcgc ggcatggtgg aggcgatcac ctggtcgttc
      541 atcgagcgca aggacgcggc cctctttggc ggtggtagcg atgcgctcga gctcgccaac
      601 ccgatctcgt ccgacatgtc gtcgatgcgg ccgacgctgc tcgtcggcct gctgaccggc
      661 gcgcagcgca atcgcaatcg cggcttctcc gacgttgcac tcttcgagat tggccaggtg
      721 ttccgcggcg accggccgga ggatcagggc caggtggccg cgggcgtgcg cgccggcgcg
      781 gcaggcatcg gcggcgcagg gcgtgattgg ctgtcacccg ccaaggatgc cggcctgttc
      841 gatgccaagg cggacgcggc ggcgctgctc tcggtgctcg gcgtcgatgt cggaaaggtc
      901 cagatcacgc gcgatgcgcc cggctggtat catccaggcc gctcgggtgt gatccggctc
      961 gggccgaaaa ccgtgctcgc ccagttcggc gaactgcatc ccgacacgct cgcggtactc
     1021 gatgtcgcag cgccagccgt ggcattcgag gggttcctgg atgcggtccc ggccgcacgc
     1081 cgcaagggga cgcaacgccc tccgctcgat gtctcggatc tccaaccggt gcggcgtgac
     1141 ttcgccttca ttctcggctc ggacgtcgcc gcggccgatg tgatccgggc ggcctccggc
     1201 gccgacaaga agctcatcac ggcggtcagc gtcttcgatc agttcgaagg cgcgagcctc
     1261 ggcgccggca agaagtcgct cgccatcgag gtcgtgatcc agccgcgcga cgagcccttg
     1321 aaggacgagg cgatcgacgc catcgccaag aaggtgatcg ccgaggtgaa gcgcgtcacg
     1381 ggcggcgagg tcaggggcgg atgaatggtg ggccggggcg cggctatcgc actattctgg
     1441 agcaagagag cggaaacagc ttgttcattt ccgccgtaca gcccgggacg cagcggatcg
     1501 atggcgccgc cttcagtttt gagagcactt ctcctgccga tacggttccg gagaggggca
     1561 gccctccatc ttgttggagt cctttgacct tcgaatccaa gatgatggtc caggcaccgt
     1621 tggcacttcc aaccggacag ccgcgagcgc gcaattcatc ctcgagatcg aggatgtctt
     1681 cgcgcgagct gctcttcgac agcatcagcc ccgtagactt cggcgctact accccgcacc
     1741 tcacggtggg ctgacgcttc atgcgagcga tcgccggcag cgtcgcgcca tggacgtcga
     1801 cgggctgcgc ggcgaccttg ttgaagtcgg tgagcgcctt gatggtcttt gcgtccatca
     1861 cacccgtcgt atccccggaa aagcagccgc ggttcctgag ctcgcgctgc agcgtgcgga
     1921 tgttctcgac gtcggcgacg gtcggcgccg gctttgctgc cgcagggggt ggaggagcct
     1981 tcggcggcgt cggttgcgga gcaggcgacg cgacgaccgg cgccgggaca ggtgtcgcta
     2041 ccacgggcgg ggcgactgct ggcgggggcg ccctcggcgg cggcatgggc ttggcgaggc
     2101 tcgagccgag tgtgggtgac ggctgcaggg gtgcctccac tctcggtggt ggcgccacgt
     2161 agatcacgtc gtagagatcg ctcgcgagct tctgatgcgt acgcgggttc atcagaacca
     2221 gtcggaactt gcgtgcgccg gtcgcaatct ccttgtgccc ttcgagccac atgatgaggt
     2281 ggatctgctg gacgtcccag taccgcgccg cctcgtgccc ctgcttctgg acatggcccc
     2341 agagcgtgtc cagcgcgaag cccatgtcct ccggattgcg gatgatgagg cgcggcacca
     2401 tggcgaggtg cggctcgatg cgggtgagga tctctttggc ctcgaccggt ttcagcacct
     2461 cgtccttcgg gtgccggaga ggattgagga ttactatccg aggcggcgat gcaccgggcg
     2521 tctcctggag catcaggatg ctggagagct cgttgatctt gaaggccagg atgtcgcccg
     2581 aataggactg cgccgcagcc ggccgggcac cgagcagcac ggcgagaatg gtcaggcatg
     2641 gcaatagctt gctcagcagc atcggcgatc cggtctcgaa gggcgttgaa accacgaagc
     2701 ttcgagggtg tcacatcgga ccacccgagc gcaatctccg tgccgagctc ggccggataa
     2761 gtgcggggcg tcagccctgg aacgcctcgt ccttcgaggc tgccccggcg gcgactgcga
     2821 cgagccgcgg gtaggcggcc cgatactcgg cgatcagggc atcgaggtcc ggctctgcga
     2881 cgaagccgtg atcggccaca tattccatgt gccgacggtt ttcacggtcg aactcatgga
     2941 agagggcgtc gctctttcca tcgaattcga gcgggtgcac gccgaaccgc tcgcagagtg
     3001 cggcattgaa cgccggcgta atcttgatcc agcgctcgtg cagccagact gccgtgtagc
     3061 cgtggttgat gaaactgtcg gtccccatgc gctcgcgcag cttgggcgtc gtcaaatggt
     3121 tgcgcacgtc cgcaaagccg acggcggcgg ggatgtccgc ggctcgtagt gccgctgtga
     3181 gcaggatcgc tttaggcacg caccacgaca cggtctgtgc cgcgacatgg ctcgccttga
     3241 acgtcacagg atctaccgag aatgagtagg ggtcgtagcg aaggccatcg cgtaccgcgt
     3301 aatagagcgc gattgtgcgg gcctcggagc tctccgatcc gacgcagctc agcgctcgcg
     3361 ctgcgaactg gcgcacttcg aaggcgtcgc tatcgacgac ggggcctgag gcaaggcagc
     3421 tgctcggatc gggtatcgga tggcgcatcc ctgccggata gcagcagccg gggccgatgt
     3481 cgattgtcga ccccgaacgt aacgccaatg tagcgtggaa ctcgcctttg gccctgagca
     3541 tgggataaac gtagtaggcc gaactgatgc tgggaggcca atatgtcgat tgcgcactgg
     3601 atgcggtttg ggttcaggcc tgcgatggtc gcggttctgc tggccgccag cgcggcgacc
     3661 gcgatcgcgc aggtcccggc aacggattcg agcatgatcg acggcctgct cggccggtca
     3721 cggacacgaa gcgccacggc ggtcgcgccg cagccgagcg ccgaggacaa ggccctgatc
     3781 gatgcgctga aatcgcggcg cacgcgcggt gtgacggtcg aggagcgcaa ggccattgcc
     3841 gtgctctccg acagcctgcc gagcatcgat gtcgagatcc gcttcgatta caattcggca
     3901 gtcatcacgg cggcggccga gccggcgctc gcgagcctcg gccgcgcgct gaacgatcct
     3961 cgccttgccg gcctatcgtt tctagtttgc ggccacaccg acgctgtcgg cggtgcggag
     4021 ttcaatctcg ggctcagtca gcgccgcgcc gaagcagtgc aacgctatct ggctgaacgc
     4081 catgctgtcg ccgccgatcg gctggcgacg gtcggccatg gcttcgagca gctgaaggat
     4141 aaggccaacc cgaaggcggc cgccaaccgg cgcgtccaga tcgtcaatct cggcggcacg
     4201 cgctcggtgg ctgcggcgcc gaggccgaag gtcgttcccg tctctgggcc accaggggcg
     4261 aaggctccnn nnnnnnnngc caagacgcgc tcactaatgg acaagtcgct cgccgatatc
     4321 gcgggcgtca cggaggtgtc ggcagcaccc cagccaccgg cggtgacgag cgcgccggcg
     4381 cccctcgtcg tgaagctgtc gcccggtggt gcgctgcggg tcggcgacct gatgaccgtg
     4441 agcatcacca gcacgattgc cggcaacctc atcgtctacg agcaggacgc ggccgggcag
     4501 acgacgcaga tcttcccgaa caacttcagc gatgcaccgc agaccacgat cgacgccggt
     4561 cagacgatca ccattccggg cccgacgagc cgcttccagc tccgtgtcac gccgccggtc
     4621 ggagcgagcc gcatcattgc ggtcgtggtt cctgcgcgcg tcaccgtcgc cgacatcacg
     4681 cgcgacggcg ccgacatgaa gccgctcgcc aacccggtcg aggtgatcgg caagatcgcg
     4741 gagcgggtca cgcgcggcgt cagggtcgag ccggccgaac gagctgtcgg gatcgccgag
     4801 tacacgatcg ccaactgagc acgcacgggg ctcaactcag caggcctgtc tcacccgctg
     4861 cacgtgcgcc gcaattcgaa gtgcggtcgc gtgcggctcg aggtcagtcg tgtcgagtgt
     4921 cagctcactg gcactgcccg tcatgagggc gccgcgactt gcattgcgtc gcgccgtgtc
     4981 ggcgtccatg ctcttcaatc gcggcgctcg gtccggcgag accacccggc tggcgagtgc
     5041 ttcgggtgcg cagtggagcc gaacgggcaa gtagggcgag ccgcgcattt cggccgctct
     5101 cgcaacttgt tgtccgagcg cccggtcgtc ctcgccatcg tagagctcgt tggtcaggat
     5161 gtagctctgc tcaggcgggc agatcaccgc gatggttgcg agcacggctt cgcgcacctt
     5221 ttcaatctcg gtccagactt gcgcgggata tggcgtgacg cggtcgtgcc gcaggaggcc
     5281 gaggaccgga ttgttgatcc agtgattgtc aaccacgatc gcatcgagca tcggcgccag
     5341 cgcacgggcg acggtcagct tgccgacgcc cgcaggtccg atgagatgca cgattgctcc
     5401 gcggctcgcc atggtgcgct cccggattga tggagtgagg cgcgattctc gtgccggcag
     5461 aggatgccag tcaagccgcg cgcaggcccc gatgatcagg ctgcccgccc ggtcggcgtc
     5521 tgatgcgacg gacccagaat tgcgcgcttg ccgccgacgc cgacgggacc aatgaggccc
     5581 tcgcgttcca tgcgctcgat gaggtcggcg gcgcggttgt agccgatgcc gaggcggcgc
     5641 tggacgtagc tcgtcgaggc cttctggtcc gccaggacga gggcgacggc gcgctggtag
     5701 aggtcgtcac cgccggcccg cactgccgcg gtgccgcgat catcgccgga ttcgggcgca
     5761 tcctccggcc cctcggtgat gccgagcacg tagtcgggcg tgccctgctc gcggaggtag
     5821 gcagcgatcc gctcgacctc ctcgtcggca acgaaggcgc cgtgaacgcg cagcgcgccg
     5881 ctgccgcctg aggaaacgag catgtcgccc atgccgagca gcgcttcggc acccatctca
     5941 ttgaggatcg tccggctgtc gatcttggag gcgaccttga acgagatccg cgtcgggaag
     6001 tttgccttga tcgtgcccgt gatcacatcg acgctcgggc gctgcgttgc catgatcaga
     6061 tggatgcctg ccgcgcgggc catctgcgcg aggcgctgca cggccgcctc gacgtccttg
     6121 ccagcgacca tcatcagatc ggcgaactcg tcgaccacca cgacgatgtg cgtcatcacg
     6181 gcgtgctcga tcgtctcggt ctcgtagacg ggctcaccgg tctgcgggtc gtagccggtc
     6241 tggacctgcc gcgagagcgg ctcgccggct tttgcggcgt tgcgcacgcg ggcgttgtac
     6301 atctcgagat tgcgcaccgc gaggctcgcc atgcgcttgt agcgctcctc catctcgcgg
     6361 acgacccagc cgagcgcggc ggcagccttc gagggatccg tgacgaccgg cgtgaggagg
     6421 tgcggaatgc cgttgtatac cgagagttcc agcatctttg ggtcgatcat caggaagcgg
     6481 caggcctccg gaccgtggcg atagaggagc gagagaatca tcgagtttac gccgacggac
     6541 ttgcccgagc cggtggtgcc ggcgacgagc agatgcggca tgcgggcgag gtccatcacc
     6601 acggggtcgc cggcaatgga tttgccaagc gctataggca aggtcgcggg gctctcgcgc
     6661 cattcgcgcg attcgaggat ctcgcgcatg gcgacggctt cgcggcgcgc attcggcatc
     6721 tcgatgccga tcacggagcg gcccggtacc acggcgatac gcaccgaggc gacactcatc
     6781 gcgcgggcaa tgtcgtcggc caggccgatg acgcgggagg ccttggtgcc gcgtgccggc
     6841 tcgagctcga acacggtgac gacggggcca gggcggatat ccttgatctc gcccttcacc
     6901 gagaagtcgg cgagcgcgtc gaggagaagg cgcgcctggc cgcgcaaggc ctgctcggcc
     6961 gttgccgcat cggggcggtg gagcacgggc ttcttcagct cgttgagaga cgggcggcgg
     7021 tagccatggc cttggtgcgc cgactcggtt cccacgtggc gcggcggata gggtcgatcg
     7081 ctcactgcat ggccggcatc gctgcgcgca ggcgcgaacc ggcgggcgat ggcgccgctt
     7141 tcgacgtccg tcgtctcctc gaaccgggga tcgatcgccg cggcggggcc tgccgatgcc
     7201 ggatcgggtg gatgcccgat gacggggagc atggacggtg caggctcgct gtcggggcct
     7261 gccgcgatcg ggggcgtcca gggcctgagc tccgacggct tggccggaat cggatcgtag
     7321 gcatcgaggc cgggtcggta atcctcgaga ttgaggcgag gcggggnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnngccg ccgcagcaaa gagcatgagc ccggccgcga cgccggcgcg
     7501 ccccgggttg atgagcgcga acagccctga gacggttttg aacgagagat cgccgatcat
     7561 gccgccatgg ccatagacga gcggccagcg tgtcaggatt ggcagagccg acagtgcgcc
     7621 ggccgtcgcg agaaggacga ggggccacag gaggagacgc gggcgc
//
LOCUS       DAORNH010000222         7656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7074_length_7656_cov_2.792264, whole genome shotgun sequence.
ACCESSION   DAORNH010000222 DAORNH010000000
VERSION     DAORNH010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7074_length_7656_cov_2.792264"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK264_18625"
     CDS             complement(<1..336)
                     /locus_tag="PK264_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77924.1"
                     /translation="MQLLLRPMILALATLVLVPTTALTQQRKLAAVIDIDRCQGIAAL
                     ERGVTDARASSETLNCLCHDVPPGGENVTGHGFVRHIQLGNQIACIFTGHACEADGRN
                     VVFARDVPKV"
     gene            complement(414..2927)
                     /locus_tag="PK264_18630"
     CDS             complement(414..2927)
                     /locus_tag="PK264_18630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77925.1"
                     /translation="MSAMNGPSTKASQHDLPARGQEAGWLLITVVLVLAGGLAGLSML
                     TSRSGEPLVLTVLALLAMIGAFFLFGLAAGYIRMGDRPDDRGFLLDVIDQSGVGIVVT
                     RPDGTPVQANAAYRAMTHGGAQSLEDVLSGEPVAAEALFRLTRAAAEGSSHSEVVRLR
                     GISGSRQAQWLRLSVDPLRTDGTYSSGDSLTIWRVLDVTSERAQEAEVRAGLEAALQL
                     YDALPFGVVTLGPSGRIITANATVLQQLGLSSPPGRLPQLIVDDVFASESVGAVKALL
                     RQPLRQTTSIDIAMLDRQGKSLPVNAVAMARQEAAHDQTLVILDKTARLTSAIDASEA
                     GRAARSFEAAPFGIAIVDPAGRILSSNAAFARLFAGRQEPHSMASLVSLFQAPAEAEA
                     RNQLLQALAQLMAGEQTGGPIDVTFGAERQFARRIYMSPITGQRSSGEAAVLFVLDTS
                     EQKSLELMFAQSQKMEAIGKFVGGIAHDFNNVLTVILGLSDFLLRNRRPTDPGYTDMM
                     QIKSNANRAANMVRQLLAFSRKQTLTPEMLDLNDVVQDFGYSLNRLLGEKITLKVSAG
                     RDLWPIKADRTQLEQVIMNLAANARDAMPGGGRLTVRTRNIGERDSQKLKGSGLGPGE
                     YVLVEVEDTGTGMSAEVMQKIFEPFFTTKEIGKGTGLGLATVYGIVRQTGGQIIPESE
                     IGKGTTFRVYLPRYIPSSDEEPSKVAKKERARDLTGSGRVLLVEDEDGVRSFASRALK
                     QQGYTVLEASSGVEALDVMKGQTVDIVVSDVLMPEMDGPTLMNELRKETPDLKFIFVS
                     GYADDALEKSLDAKAQFAFLPKPFTLPQLAAKVKEELGK"
     gene            3335..3709
                     /locus_tag="PK264_18635"
     CDS             3335..3709
                     /locus_tag="PK264_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HRD77926.1"
                     /translation="MLFYFFILVVVAALALAAIFAVRSYLDGSAPGSGLFGPRAERRL
                     AVVDQASVDGRRRLILVRRDDVEHLIMTGGPVDVVIETGIGAPKAAAVPAPSTVYARQ
                     PRPFGQTAATASATAASDRAAE"
     gene            3758..>4135
                     /locus_tag="PK264_18640"
     CDS             3758..>4135
                     /locus_tag="PK264_18640"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD77927.1"
                     /translation="MRNFHLPGRSPVYAHRAMCATSHPLASEAAIRVLRSGGNAVDAA
                     ITATGVLCVVEPAMTGIGGDCFAIVRKPDGTILGLNASGRAPAAATAAWYAERGITTI
                     EQTTPHAVTVPGAIRGWAKLLADH"
     assembly_gap    4136..4204
                     /estimated_length=69
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4205..5343
                     /locus_tag="PK264_18645"
     CDS             <4205..5343
                     /locus_tag="PK264_18645"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD77928.1"
                     /translation="RVSHDWAGLVAKLQKNEGARRHLLIDGRAPRVGERMRMPALAVT
                     LEKIAREGAEAFYAGDVAEDMVRDLRELGGLHTLADFAAQDAEYVTPIATTYRGIALH
                     ELPPNNQGIVALVVLRMLERLGPLGTDPIGVERIHATMEVARLAYAVRDTFVADPAMA
                     EIPVAYMLSDAFIDGLVRRIDRKRRIAELGPIPEPKGSDTIYMTVVDERGMAVSFINS
                     LFAGFGSGIVARKSGITLQNRGQGFVLTPGHRNCIAPRKRPLHTLVPAIATEGKSLWA
                     TFGVMGGAYQPFGHAWVLTNMIDYGLDPQEAVDCPRVFFEGQAVQVEDGVPLASRAAL
                     EAMGHKVERRADPWGGSQLIRYDAALATYIGASDPRKDGQAIGY"
     gene            5749..7434
                     /locus_tag="PK264_18650"
                     /pseudo
     CDS             5749..7434
                     /locus_tag="PK264_18650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
     gene            7350..>7656
                     /locus_tag="PK264_18655"
     CDS             7350..>7656
                     /locus_tag="PK264_18655"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRD77929.1"
                     /translation="MGRQRREPADEQDHWWQHPDPRLAWLHVGRTYQHGHSDHPRLAD
                     ASQSDRRAPAARGVEADRRRHRYDTADYYQIDPALGTLADFQELVQAARANGMRLILD
                     "
BASE COUNT         1244 a         2469 c         2578 g         1296 t
ORIGIN      
        1 aaccttcggc acatcgcgcg cgaacaccac gttacgccca tcagcttcgc atgcatggcc
       61 ggtaaagatg caggcgatct ggttgccgag ctggatgtga cggacgaacc catggccggt
      121 gacgttctcg ccgcccggag gcacgtcgtg gcagaggcaa ttcagcgtct cggaacttgc
      181 gcgggcgtct gtcacgccgc gctcgagcgc ggcgattccc tgacagcggt cgatgtcaat
      241 gactgcggca agcttcctct gctgtgtcag cgcggtcgtc ggaaccagga cgagcgtggc
      301 cagcgcgagt atcatcgggc gcaaaagcaa ttgcatgggg cctccacgac gagaggtcgc
      361 atcatgccaa accgggcccg gcgcgtgaat ggcaaaatcg ggtcgccacc acgctatttc
      421 ccgagctctt ccttcacttt ggcggcgagc tgcgggagcg tgaagggctt cggcaggaag
      481 gcgaactggg ccttggcatc gaggctcttc tcgagggcgt cgtcggcgta gcccgagacg
      541 aagatgaact tgaggtcggg cgtctccttg cggagctcgt tcatcaaggt cgggccgtcc
      601 atctcgggca tcagcacgtc ggagacgacg atgtcgacgg tctggccctt catgacatcc
      661 aatgcttcga caccgctcga ggcttcgagc acggtgtagc cttgttgctt cagagcgcgc
      721 gaggcgaaac tcctcacgcc gtcctcgtcc tcgacgagca gcacgcggcc tgaacccgtg
      781 agatcgcgcg cccgctcctt cttggcgact ttgctcggct cctcgtcgga gctcgggatg
      841 tagcgcggca ggtagacgcg gaaggtcgtg cccttgccga tctcgctctc ggggatgatc
      901 tggccacccg tctggcgcac gatgccgtag accgttgcga ggccgaggcc cgttcccttg
      961 ccgatctcct tcgtcgtgaa aaacggttcg aagatcttct gcatcacctc ggccgacatg
     1021 ccggtgccgg tgtcttccac ctcgacgagg acatattcgc cgggtccgag gccagagccc
     1081 ttgagcttct ggctgtcacg ctcgccgatg ttgcgcgtgc gtacggtgag gcggccgccg
     1141 ccgggcatgg catcacgcgc attggcggcg aggttcatga tcacctgctc aagctgcgtc
     1201 cggtcggcct tgatcggcca gaggtcgcgc cccgccgaga ccttgagcgt gatcttctcg
     1261 ccgagcagcc ggttgagcga gtagccgaag tcctgcacga cgtcattgag gtcgagcatc
     1321 tcgggcgtga gcgtctgctt ccgcgagaag gcgagcagct ggcgcaccat gttggcggcc
     1381 cggttggcgt tcgacttgat ctgcatcatg tcggtgtagc cggggtcggt cgggcggcgg
     1441 ttgcgcagga ggaaatccga gaggccgagg atcacggtca gcacattgtt gaagtcgtgg
     1501 gcgatgccgc cgacgaactt gccgatcgcc tccatcttct ggctctgcgc gaacatcagc
     1561 tcgagcgact tctgctcgct cgtatcgagc acgaacagca cggccgcctc gcccgatgag
     1621 cgctgacctg tgatcgggct catgtagatg cggcgcgcga attggcgctc ggcaccgaag
     1681 gtgacgtcga tcgggccgcc ggtctgctcg ccggccatga gctgcgcgag tgcctgcagc
     1741 agctggttgc gtgcctcggc ctcggccggc gcctggaaga ggctgacgag gctcgccatc
     1801 gaatgcggtt cctggcggcc cgcgaacagc cgggcgaagg cggcgtttga gctgaggatc
     1861 cgaccggcgg gatcgacgat ggcgatgccg aagggggccg cctcgaagct gcgggccgca
     1921 cgccctgctt ccgatgcatc gattgccgag gtcaggcgcg ccgtcttgtc gaggatgacc
     1981 agcgtctggt cgtgcgccgc ctcctggcgc gccatggcga cggcgttgac ggggagcgat
     2041 ttcccttgcc ggtcgagcat ggcgatatcg atgctcgtcg tctggcggag cggctggcgc
     2101 agcagcgcct tgaccgcgcc gacgctctcg ctggcgaaga cgtcgtcgac gatcagctgc
     2161 gggaggcgtc cgggcgggct cgacaggccg agctgctgca ggactgttgc attcgccgtg
     2221 atgatgcggc cgctcgggcc gagcgtgacg acgccgaacg gcagggcgtc gtagagctga
     2281 agggcggctt cgagcccggc ccggacctcg gcctcctggg cgcgctcgct cgtcacgtcg
     2341 aggacgcgcc agatggtgag gctgtcgccg ctcgagtagg tgccatccgt gcgcagcgga
     2401 tcgaccgaga ggcggagcca ttgcgcctgg cgcgaaccgc ttatgccgcg gagccgcacg
     2461 acttcggaat ggctgctgcc ttcggccgcc gcgcgcgtca ggcggaacaa ggcctcggcg
     2521 gcaaccggct cgcccgagag cacgtcctca agcgattgcg cgccgccgtg ggtcatggcg
     2581 cgataggcgg cgttggcctg gacgggcgtt ccgtccggcc gtgtgacgac gatgccgacg
     2641 ccggactggt cgatcacgtc gagcaggaag ccgcggtcat cgggccggtc gcccatgcgg
     2701 atgtaaccgg cggcgagccc gaagagaaag aacgcaccga tcatcgcaag aagtgccagc
     2761 acggtgagca ccaggggctc gcccgaacgg ctcgtgagca tggaaagtcc ggcaagcccg
     2821 ccggcgagca ccagaacgac ggtgatcagc agccagccgg cctcctggcc acgcgccgga
     2881 agatcgtgct gcgacgcctt ggtcgagggg ccgttcatcg cgctcatgat tcgaactaat
     2941 agctgattcg ctgtcgacgc actgcgcgat tgggtcattt gtacaggtca ctcagaaggt
     3001 tactgtgagt tgtgcacacc ttgggtccgg cgatggcaac gcttcgatcc gcacgcggtc
     3061 gaaaggcgtc gctgcccgta aagagatcgg tgaattaggt taagttgcca tgaattccgg
     3121 tgcgccggcg gcacactggc cacgggcttt gcgagtttcg cccggccgcg cggttaagcg
     3181 gcgttgacct tctccgccgt tcgcgcgaca cggaccctcg agacaatgtt tgagcgtcag
     3241 tcgggtacgc gggtcgagtg atccggcggc ggccatgcgg ggcggctgca atcggcggcg
     3301 cttggcacgg cttgaggagg caaccttggc ggacatgctg ttctacttct tcattctcgt
     3361 tgtagttgca gccctggcac tggcggcgat cttcgccgtg cgcagctatc ttgacggctc
     3421 ggcgccgggc tcgggcttgt tcggtcctcg tgccgaaagg aggctcgctg tggtcgatca
     3481 ggcttcggtg gacgggcggc ggcgcctcat actcgtcagg cgcgacgacg tcgagcacct
     3541 catcatgacg ggtggtccgg tggacgtggt gatcgaaacc ggcatcggcg cgcccaaggc
     3601 tgccgcggtt ccggccccca gcacggtcta cgcccgtcaa ccgcgcccgt tcggccagac
     3661 ggccgcaacg gcgtcggcga cggcagcttc ggacagggca gcggagtagg tcggagtgat
     3721 ctccggcgcg tcacgatttc cggcaaggat cagcgacatg cgcaattttc atctccccgg
     3781 gcgctctccg gtctacgcgc atcgcgcgat gtgcgcgacc tcccatccgc tcgccagcga
     3841 ggcggcgatc cgcgtcctac gctccggcgg taacgcggtc gacgcggcga tcacggcgac
     3901 gggcgtgttg tgcgtagtcg agcccgcaat gacggggatc ggcggcgact gcttcgcgat
     3961 cgtccgcaag ccggatggca ccatcctcgg tctcaatgcc tcggggcgtg cgcctgccgc
     4021 ggcgacagct gcgtggtacg ccgagcgcgg catcacgacg atcgagcaga cgacgccgca
     4081 tgccgtcaca gtgccgggcg cgatccgcgg ctgggcgaag ctgctcgcgg accacnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnncccgcg tctcccacga ctgggccggc ctcgtcgcca agctgcagaa gaacgagggg
     4261 gcccgtcgcc atctcctgat cgatggccgc gcgccgcggg tcggcgagcg tatgcgcatg
     4321 ccggccttgg ccgtgacgct cgaaaagatc gcgcgggagg gtgccgaggc gttctacgcg
     4381 ggcgacgtcg ccgaggacat ggtgcgcgat ctccgcgagc tcggcgggct ccacacactc
     4441 gccgatttcg ccgctcagga cgccgagtac gtgacgccga tcgccacgac ctatcgcggc
     4501 atcgcgctgc atgagctgcc gcccaacaac cagggcatcg tcgccctcgt tgtgctgcgc
     4561 atgctcgagc ggctcgggcc actcgggacc gatccgatcg gcgtcgagcg tattcatgcc
     4621 accatggagg tcgcccggct cgcctatgcg gtgcgcgaca cgtttgtcgc cgatcccgcc
     4681 atggccgaaa ttcccgtcgc ctacatgctg tcggacgcct tcatcgacgg gctcgtgcgc
     4741 cgcatcgacc gcaagcggcg catcgccgag ctcgggccca tcccggagcc gaagggttcg
     4801 gacaccatct acatgactgt cgtcgacgag cgtggcatgg cggtctcgtt catcaattct
     4861 ctgtttgcgg gcttcggctc ggggatcgtg gcgcggaaat ccggcattac tctacagaac
     4921 cgcggccagg gcttcgtgct gacgcccgga caccgcaact gcatcgcgcc gcgcaagcgc
     4981 cccctgcaca cgctcgtccc cgcgattgcg accgagggca agagcctgtg ggcgacgttc
     5041 ggcgtgatgg gcggcgccta ccagcccttc ggccacgcct gggtgctgac caacatgatc
     5101 gactacggcc tcgatccgca ggaggcggtc gattgcccgc gcgtgttctt cgagggccag
     5161 gcggtgcagg tcgaggacgg cgtgccgctg gcgagccgag cggcgctcga ggccatgggg
     5221 cataaggtcg agcgccgcgc cgatccctgg ggcggcagtc agctcatccg ctatgatgcg
     5281 gcgctcgcca cgtatatcgg cgcctccgac ccgcgcaagg acgggcaggc cattggctac
     5341 tgacggtgcc gggtgcaggt tccgctgccc atcgaccaca gtggatgctc gcaatgccgc
     5401 cacgaggctg gtagatgagt agatcgaaac gcctcggatg aactcgcccg agcgcggagc
     5461 gcgatcgacg ggagagtttg cggtcctgga cggcgtgagc gtggctcagg aaggacttgg
     5521 gctttgaacg actggttcta caggcgcgag aacaagaagc gcgggctcgt caatctgctc
     5581 ggcatcgatt cctggatcga ctcgagtctt gcgagcgcgt gggagcgatt catcgaccgc
     5641 tggaacgcag ccgcgagctt ctttgcgagg tttcgcctct cgggttggaa gcgcatcctg
     5701 aacgagctcg tctcggaggg tatgacgctc ggcatctgcg gcatggcgat gctctattcg
     5761 ctggcgctgc cgaccatctc cgaaatcgac gaggaccgct atctcgacac cggcaagtac
     5821 agcgtgacct tcctcgatcg caacggcaac gagatcggca agcgcggcgt cctgcattcc
     5881 gacgccgtcg aactcgagga cattccggac tacctgatca aagctgtgct cgcgacggag
     5941 gatcgtcgct tcttcgagca ctacggcgtc gactttctcg gaacggcgcg cgcgctaatc
     6001 gagaacctgc gggcgggcga gacggtacag ggcggctcga cgctgacgca gcagctcgcc
     6061 aagaacctgt tcctctcctc cgagcgatcg ctgcagcgca aggtcaagga gctgtttctt
     6121 tcgttctggc tcgagactcg cctgacgaag cgccagatca tgaagctcta cctcgaccgc
     6181 gcctacatgg gcggcggcgc ggtcggcgtc gaagccgcct cgcgcttcta tttcggcaag
     6241 tccgtgcgcg agatcaccat ggccgaggca gccgtgctcg cggggctctt caaggcgccg
     6301 accaagtatg cgccgcacgt caacatctcg gcctcgcgct cgcgtaccaa cgatgtgctc
     6361 tcgaatatgg tcgaggccgg attcttctcg gccggccaag tgcacgaggc gcgcctcaat
     6421 ccggcccgcc acattgagac gcgcgcgctc tacagcccgg actggttcct cgattgggcg
     6481 ttcgaggaga tacagcgcgt cgccgagggc cgcggacatt tcgtgctgac cgcacgcacg
     6541 acgatcgaca tcgccttgca gcaggccgcg gaggcagccc tcgtctcggt catccgccag
     6601 cacggcgtga accagggcgc gcgcacggga gcgctggtca gcatggagcc ggacggcgcc
     6661 gtccgcgccc tcgtcggcgg tctcgactac ggcgagagcc agttcaatcg cgccactcat
     6721 gcgcgccggc agcccggctc gtcgttcaag ctctacgtct atgctgcagc cctcgagttc
     6781 ggcctgacgc cacggtcgaa cgtgcgggac agccccgtgt cgtgcgggaa ctggtcacca
     6841 cagaactatg acggcggatc gggcagcggg cggacgctca cgctcggcag cgcgctcgcg
     6901 gtgtccttga atacggtggc cgtcgaactg tcgctgcgcg tcgatcgaga gaaggtcgtc
     6961 gagatgacgc gccggctcgg cgtcgagggc gtgcgcaaga cctgctcgat ggcgctcggc
     7021 gataccggca tcaccacgct gcagcacacg gcggcctacg cggcgtttgc caacggcggc
     7081 aagctcgcca agccctacgc gatcctcgag atcttcaact cgaagggcga gcgcatctac
     7141 gcgcgtgagc aggacgaggc gccggcaccg caggtgctcg agccgcgggt cgccgaggcc
     7201 atgaatcaga tgttgcagct ggttgtgacc gagggtaccg gcaagaaggc gcaactcgag
     7261 ttcacccaca gcgtcggcaa gacgggcacc agctcgagct accgcgacgc ctggttcgtc
     7321 ggctacacgg gcagcctggt caccggtgtg tgggtcggca acgacgagaa ccggccgatg
     7381 aacaagatca ctggtggcag catcccgacc ctcgtctggc atggcttcat gtcggccgca
     7441 cataccaaca tggacattcc gaccatcccc ggcttgccga cgcatcccaa tcagatcgcc
     7501 gagcgccagc ggctcgcgga gttgaagcgg accgaaggcg ccaccgctac gacacagccg
     7561 actactacca gatcgacccg gccctgggca cgctggccga tttccaggaa ctggtgcagg
     7621 cggcccgcgc caacggcatg cgcctgatcc tggacg
//
LOCUS       DAORNH010000223         7640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7091_length_7640_cov_2.450758, whole genome shotgun sequence.
ACCESSION   DAORNH010000223 DAORNH010000000
VERSION     DAORNH010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7091_length_7640_cov_2.450758"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK264_18660"
     CDS             <1..216
                     /locus_tag="PK264_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD77930.1"
                     /translation="GALELGTLLSGAVLTEQVFSIPGFGKLIVDSVFNRDYAVVQGVT
                     LVTATTYILLNLLADIAYMLVNPRLRD"
     gene            335..1543
                     /gene="mnmA"
                     /locus_tag="PK264_18665"
     CDS             335..1543
                     /gene="mnmA"
                     /locus_tag="PK264_18665"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRD77931.1"
                     /translation="MARVTLDALLSPETLLPGVAPGARVVVAMSGGVDSSVVAGLLKH
                     AGFDVVGVTLQLYDHGAATSRKGSCCAGEDVHDARQVAARLGIPHYVLDYEDRFRRAV
                     IEPFAESYSEGETPVPCVACNQHIKFTGLLETAEELGASVMATGHYVGYDLRPDGRPR
                     LLRALDADRDQSYFLFATTQDQLRRLRFPLGALRKSEVRAMAGRLGLAVAEKADSQDI
                     CFVPEGRYAAVVARIHPEALEPGEIVHVDGRLLGRHAGIGAYTIGQRRGIGVAASEPL
                     FVVRLEPRTRRVIVGPRANLAVRRLRLRNVNWIGDRPIEEATRGGCNVYVRVRSSQAP
                     IAATLFADHSGICVVLPSAILGVAPGQACVFYASDMGVAEVLGGGWITSTTSEPCPMS
                     TGARDAAAVA"
     gene            1618..2310
                     /locus_tag="PK264_18670"
     CDS             1618..2310
                     /locus_tag="PK264_18670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD77932.1"
                     /translation="MKLLRKQTGARGTRGAVVTMDEDTVKSAYRRWAPVYDHTFGKFA
                     SRGRRHAVEIINQRQGRVLEVGVGTGLSLPDYERHLEIVGIDLSPEMLEKARERVASE
                     GLDHVTSLHEMDASALSFKDESFDTTVAMYVMTVVPEPEKVMRELDRVTKVGGEVMLV
                     NHFSQTEGVRGWVERRMAPFADKLGWHPVFDQGRVMVCDNLELVEARALRPWGIFTML
                     RFAKRRPTLVRA"
     gene            complement(2419..3015)
                     /locus_tag="PK264_18675"
     CDS             complement(2419..3015)
                     /locus_tag="PK264_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77933.1"
                     /translation="MRTSPPSNSSSKTSPRVGGQLQNFISDHLVAGVGRSGSVAVIAR
                     STASPVIEALSRHAPALAGTCSSLRIVVAEFCRKDAAALLHTFTGISGTGSAGAEIRH
                     ACRPRLIDAHEQLVFGDRWCWYGDSMRRDPRKRDAFESFANGNTELTRLAWLSFNRFW
                     QTADIVAIGDVDRAAVRVSSDPARTTIELPNREPLQRQ"
     gene            3306..3806
                     /gene="secG"
                     /locus_tag="PK264_18680"
     CDS             3306..3806
                     /gene="secG"
                     /locus_tag="PK264_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRD77934.1"
                     /translation="MQLALLVLHLLIAAALIAVVLLQRSEGGGLVSSGGNPMAGRGSA
                     NVLTRMTAWLATAFFATSIVLTLLAQHGSRGRSILDEPAAKGAPAKSGPALPTPSVPT
                     PAPASPGPTAPAPTDAKSPTPAPSTPLPSLPPAPGAPAAPAPSGTAPAPASPAPAAPG
                     TPPRSN"
     assembly_gap    3913..4046
                     /estimated_length=134
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4047..5070)
                     /locus_tag="PK264_18685"
     CDS             complement(<4047..5070)
                     /locus_tag="PK264_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRD77935.1"
                     /translation="MHTVDTEAAIGRLMRFLAVPGVTGRESLIAADVTAALKEAGVPA
                     KAIRLDDANKRIPVPTETGNLIVELPGRGRLSNADRVMFMTHLDTVPLCAGAVPKRSG
                     NKIVNAAKTALGGDNRTGCAVLVTLMAELNRQKLDHPPITLLFTVREESGLHGARHVD
                     MKDLGPIKQCFNYDGRSAAYVTVGAVGADKWEVEIMGRAAHAGGAPERGISSTMIMAL
                     ALAEVRAKGYFGKVVKPHGAGTSNVGPVGGKDGGTAGDATNVVTDFVRVLGESRSHDA
                     KFFKEITAAYRSAFETAAATVTNTDGKAGKVKFKTETNYHPFKLKENAAVVKRASEAV
                     AAIGRTP"
     gene            5217..5594
                     /locus_tag="PK264_18690"
     CDS             5217..5594
                     /locus_tag="PK264_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRD77936.1"
                     /translation="MSADLKAVEAVVWTYLDGLHEGDAAKIAKAFHEASHLYSRGEDG
                     SLADLPRAKWLDMIASRPSPKSKGIARTDRIVSIDFAGPESAFVKVECSIHPRYFTDY
                     LALLKLADGWRIVAKSFRTDVRA"
     gene            complement(5609..6922)
                     /locus_tag="PK264_18695"
     CDS             complement(5609..6922)
                     /locus_tag="PK264_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HRD77937.1"
                     /translation="MLQPPWLAEAWTELGQREIGGSGANARIRAMFRDAGHPAITSDE
                     TAWCAAFVGACLARSGLEPTRSLLARSYLGWGFATDEPEFGAIVVLSRTSDPSLGHVG
                     FLIGATPSAVILLGGNQADSVSVEAFPRERVLSFRRPPEAELSQTPATESAFSIALAH
                     VLEREGGWSDDPYDPGGPTNQGITLATYARFVGREVTAERLAALKAQLRAIPRSVVET
                     IYRRNYWLPARAADLPPGLQLMHFDAAVNHGVGGAARFLQECLAVTIDGEIGPETLSA
                     AAIAPLDEALARYADIRRRRYRALPHFWRFGRGWLARVDATLAAAHAAAVIPLPSQQK
                     DVPTLNTPNTPRPASTASPQTEPKWWGHSLTLWGAAITTLSTVLPVIGPFLGLDITAE
                     LVRQIGAEIVTAAQAIGGLAGTFMTVFGRIRATAPLARKPVALKL"
     gene            complement(6963..>7640)
                     /locus_tag="PK264_18700"
     CDS             complement(6963..>7640)
                     /locus_tag="PK264_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77938.1"
                     /translation="RGSLASITDAALLSGANAAAIETAPDRWEVVQFAAATLAAPLTY
                     SLSRFLRAQAGSDDAMPASVSAGARFVLLDAAVARVDLSAADVGLAISWRYGPATQPL
                     SSPGYVTETHAFSGRGLRPLSPCHVRGQRASGDLALTWVRRTRIGGDNWEQVEVPLGE
                     DSERYEVDILDGTTVKRTIAAVTPSASYTAAEQTADFGAPQPSLTVRVFQLSATAGRG
                     TPRLTTL"
BASE COUNT         1200 a         2349 c         2667 g         1290 t
ORIGIN      
        1 ggggcgctcg agctcggcac gctgctctcg ggcgcggtgc tgaccgagca ggtgttctcg
       61 atcccgggct tcggcaaact gatcgtcgac agcgtgttca atcgggacta cgcggtggtg
      121 cagggtgtca cgctggtgac cgcgacaacc tacatcctgc tcaacctcct ggccgacatc
      181 gcgtatatgc tggtcaatcc gcgcctcagg gactgagcca aagtcttgtc cgccatgcag
      241 cggaaacggc ggcgaaagtg ttgtgcgact atcgaaaatc actatagtgc cgccccggcg
      301 gcgatcgccg caccagatct gacacgtccg agtgatggcc cgcgtgaccc tcgacgcgct
      361 gctgagccca gagaccctgc tgcccggtgt cgcgccgggc gctcgcgtcg tcgtggcgat
      421 gtcgggcggc gtcgactcct cggtggttgc cggcctcctg aagcacgccg gcttcgatgt
      481 cgtcggcgtc accctccagc tctacgatca tggcgccgcg acgagccgca agggatcatg
      541 ctgcgcgggc gaggacgtgc acgatgcgcg gcaggtggcg gcgcgcctcg gcatcccaca
      601 ctatgtgctc gactacgagg atcgcttccg ccgcgccgtg atcgagccgt ttgcggagag
      661 ctactctgag ggcgaaacac ctgtcccctg tgtcgcctgc aaccagcaca tcaagttcac
      721 gggcctgctc gagaccgccg aggaactcgg cgcgagcgtc atggcgaccg gccactacgt
      781 cggctatgac ctccggccgg atggccggcc gcggctgttg cgggcgctcg acgccgaccg
      841 tgaccagagt tattttcttt ttgcgacgac gcaggaccag ctccgtcgcc tcaggttccc
      901 gcttggcgcg ctgcgcaaat ctgaggttcg cgccatggcg gggcggctcg gcctcgctgt
      961 cgcggagaag gccgatagcc aggacatttg ctttgtaccg gaaggccgtt atgcggccgt
     1021 cgtcgcacgg atccaccccg aggctctgga gcccggcgag atcgtccacg tcgacggccg
     1081 cctgctcggg cgtcatgccg gcataggggc ctacaccatc ggccagcggc gcggcatcgg
     1141 ggttgctgcg agtgagccgc tgttcgtcgt ccggctcgag ccgcggacac gacgcgtgat
     1201 tgtcggcccg cgggcgaacc tggctgtgcg ccgtctgcgg ctccgtaatg tcaattggat
     1261 tggcgatcgt ccgatcgagg aggcgaccag aggcggctgc aacgtctatg tgcgcgtgcg
     1321 ctcgtcgcag gcgccgattg ccgccacact ttttgccgat cattcaggta tctgcgttgt
     1381 gctcccgagc gcgatactcg gtgttgcgcc cggtcaggct tgcgtgttct acgcgtctga
     1441 tatgggtgtc gctgaggttc tagggggcgg gtggatcaca agcacgacgt cggagccctg
     1501 tccgatgtca accggggcta gagatgctgc cgcggtcgcg taagcgccgt gacggcattg
     1561 tttcgcgcgg ggttgacccg cagacgcggc ttttgagatc gaaggagact tagggggatg
     1621 aagctgttgc gtaagcagac aggcgcacgt ggcacccgcg gcgccgtcgt gaccatggac
     1681 gaggacacgg taaagtcggc ctatcgccgt tgggcgccgg tctacgacca cacgttcggc
     1741 aagttcgcct cccgcggtcg caggcatgcc gtcgagatca tcaatcagcg gcaggggcgc
     1801 gtgctcgagg tcggcgtcgg tactggactt tcgcttcccg actatgagcg ccacctcgag
     1861 atcgtcggca tcgatctttc gcccgagatg ctcgagaagg cgcgtgagcg cgtcgcgtcc
     1921 gaggggctcg atcacgtgac gagcctgcac gagatggacg cgagcgcgct gagcttcaag
     1981 gatgagagct ttgacacgac ggtcgccatg tacgtcatga cggtcgtacc ggagcccgag
     2041 aaggtaatgc gcgaactcga tcgcgtcacc aaggtcggcg gcgaggtgat gctcgtcaat
     2101 cacttcagcc agaccgaggg cgtgcgcggc tgggtcgagc ggcggatggc gccgtttgcc
     2161 gacaagctcg gctggcatcc cgtgttcgat cagggccgcg tcatggtgtg tgacaacctc
     2221 gagctcgtcg aggcgcgcgc gctccggccc tggggcatct tcacgatgct gcgctttgcc
     2281 aagcggcgtc cgaccctggt gcgggcttag cagcaaacga ttgcgtcggc gacgacagat
     2341 cctgcaagca aaaccgcctc gaagctcgac gcttcggggc ggttttgctt gcggggcgag
     2401 gcagccatca atagcgcgct actggcgctg gagcggctct cggttcggca gctcgatcgt
     2461 cgtgcgggcg gggtcactgc tgacccgcac tgcagcgcga tcaacgtcac cgatcgcgac
     2521 gatgtcggcc gtctgccaga atcggttgaa cgacagccag gcaagccgcg tgagctcggt
     2581 attgccattg gcgaagctct cgaaggcatc gcgcttgcgc gggtcgcggc gcatgctgtc
     2641 gccgtaccag caccagcgat cgccgaacac cagctgctcg tgagcgtcga tgaggcgcgg
     2701 acggcacgca tgccgaattt cagcgccggc actaccggtg cctgaaatac ccgtaaacgt
     2761 gtgcagcagt gccgcggcgt ccttgcggca gaactcggca acgacgatgc gaaggctcga
     2821 gcaggtgccg gcgagcgcgg gtgcgtgccg ggacagagct tcgatgaccg gtgaggcggt
     2881 ggaacgcgca atgacggcga cgctgccaga gcggcctacg cctgcgacga gatgatcaga
     2941 aatgaaattc tggagctgac ctccgacgcg tggactggtt ttgctgctcg aatttgaggg
     3001 gggagacgtg cgcatctatg tttgtccctc gcggtgcgcc cgggcgggca agcactctgt
     3061 tctcgttgac ctgactattt gcggccaatg cggttaaaaa gtgattgagc gatggcccga
     3121 tccggaccat ccacacgggt gcttttccga tcatgccgca cctggagtgc ggagccattt
     3181 tgcccaagtt cgaggggcgg tcccgccgac gaggtgcgac tgccatcgga tcgcacttgg
     3241 ttttgaggcg cgcagtgtgt agaagcacgc gagccgtttt ccctgccgtc ccggaaccgc
     3301 cagccatgca acttgcactt ctcgtcctcc acctgctcat cgcggccgcg ctcattgccg
     3361 tagtgctgct gcaacgatcg gaggggggcg gcctcgtctc aagtggcggc aacccgatgg
     3421 ccggacgtgg cagcgcaaac gtgctgaccc gcatgaccgc ctggctggcg acagccttct
     3481 tcgcaacgag tattgtgctc accttgttgg cccagcacgg ctcgcgtggt cgatcgatcc
     3541 tcgacgagcc ggctgccaag ggcgcgccgg caaagtctgg gccggccctg ccgacaccct
     3601 cggtaccgac gccggcgcct gcgtcccccg gaccgaccgc gcctgctccg actgatgcta
     3661 agtcgccgac cccagcaccg tccacgcctc tgccgtctct gccgcccgcg cccggcgcgc
     3721 cggctgcgcc tgctccatcc ggtaccgcgc ccgcaccggc gagcccggca ccggcagcac
     3781 ccggcacacc gccgcgctcg aactgagcgt cgactgactg gatggaacag cgaggccgga
     3841 gcgtgctccg gcctcgatct cgttcaggcg ccgccgatgt gcatcaatcg caacacgctg
     3901 ttcgcgcgca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnncggg tgtgcgacca atggcggcga cggcctcgct
     4081 cgcgcgcttc acgacggcgg cgttctcctt gagcttgaag ggatggtagt tcgtctcggt
     4141 cttgaacttg accttgccgg ccttgccgtc ggtattggtg acggtagctg ccgccgtctc
     4201 gaacgccgat ctgtaggcag cagtgatctc tttgaaaaat ttcgcgtcgt ggctgcggct
     4261 ctcgccgagc acgcggacaa agtcggtgac gacattggtc gcatcgccag ctgtgccgcc
     4321 gtccttgccg ccgacggggc cgacattact cgtgccggcg ccatgcggct tcacgacctt
     4381 accgaagtag cccttggcgc gcacctcggc gagcgccagc gccatgatca tcgtcgagct
     4441 gatgccgcgc tcgggcgcgc cgccggcatg agctgcgcgg cccatgatct cgacctccca
     4501 tttgtcggcg ccgaccgcgc cgacggtcac gtaggccgcc gagcgcccgt catagttgaa
     4561 gcactgcttg atgggcccga gatccttcat gtcgacgtgg cgggcgccat ggaggccgct
     4621 ctcctcgcgc accgtgaaaa ggagagtgat tgggggatga tcgagcttct ggcgattgag
     4681 ctccgccatc agcgttacga gcacggcgca gcccgtccgg ttgtcgccgc cgagcgcggt
     4741 cttcgcggcg ttgacgatct tgttgccgga gcgcttcggc acggcaccgg cacagagggg
     4801 gaccgtatcg aggtgcgtca tgaacatgac gcggtcggca ttcgagaggc ggccgcggcc
     4861 cggcagctcg acgatgaggt tgccggtctc ggtcggcact gggatgcgct tgttggcgtc
     4921 gtcgaggcga atggccttcg ccggaacgcc ggcttccttg agggcggcag tcacgtcggc
     4981 ggcaatgagg gattcgcgcc cggtcacgcc cggcacggcg agaaagcgca tcaggcggcc
     5041 aatcgcggcc tcggtatcga cggtgtgcat ggggctcccc gaataggttg agcccaagtt
     5101 ctggcacgcc ttctcgtgcc ccgccaacca gcgacgacta tcccggaggc agataaggat
     5161 tgcgcgcgcg gaacagctga tgaaaatgca gtccgagttc aagagtggag gcgaccatga
     5221 gcgcagatct gaaagccgtc gaggctgtgg tatggaccta tctcgacggg ctccacgagg
     5281 gcgacgcggc aaaaatcgcg aaggcctttc acgaggcgag ccacctctat tcgcgcggcg
     5341 aggacggcag cctcgccgat ctgccgcgtg cgaagtggct cgacatgatc gcgagccgtc
     5401 cgtcgccgaa gtcgaagggg atcgcgcgta cggatcgcat cgtgtcgatc gatttcgcgg
     5461 ggccagaatc ggcctttgtc aaggtcgagt gctcgatcca cccgcgctac ttcacggact
     5521 acctggcgct gctcaagctt gccgacggct ggcggatcgt cgccaagtcg ttccggacag
     5581 acgttcgagc ttgagagaga gatcgtgatc aaagcttcag agccaccggc ttgcgggcga
     5641 gcggggcggt ggctcggatg cggccgaaca cggtcatgaa cgtgccggcg aggccgccga
     5701 tcgcctgtgc ggcggtgacg atctcggcgc cgatctggcg aacgagttcg gcggtgatgt
     5761 caaggccgag gaaggggccg atcactggga gcactgtcga gagcgtggtg atcgcggcgc
     5821 cccagagagt gagcgagtgg ccccaccact tgggttcagt ctgtgggctt gcagtcgagg
     5881 cggggcgcgg tgtgttgggg gtgttcaagg tcggtacgtc cttctgttgt gagggtaggg
     5941 gtatgacggc cgcagcatgg gcggcggcga gcgtcgcatc gacgcgcgcg agccagccgc
     6001 ggccgaaccg ccagaagtgc gggagcgcgc gatagcgtcg gcggcggatg tcggcgtagc
     6061 gggcgagcgc ctcgtccagc ggggcgatag ccgcggcgct gagggtctcc ggtccgatct
     6121 cgccgtcgat ggtgactgcg aggcattcct gcagaaagcg cgcggcaccg ccgacgccgt
     6181 gattaacggc ggcatcgaag tgcatgagct gcaggcccgg cggcagatcg gcggcgcgtg
     6241 cgggcagcca gtaattgcgg cggtatatgg tctcgacgac ggaccgcgga atggcgcgga
     6301 gctgcgcctt gagggccgca agtctttcgg ccgtcacctc gcggccgacg aagcgggcgt
     6361 aggtggcgag cgtgatgccc tggttggtcg ggccgccggg gtcatacggg tcgtcgctcc
     6421 agccgccttc ccgctcgagc acgtgagcga gtgcgatcga gaacgcgctt tcggtcgctg
     6481 gggtttgcga aagttcagct tccggcggac gccggaagga gaggacgcgc tctcgcggga
     6541 acgcttcgac ggagacgctg tctgcctgat tgccgccgag caggatcacc gctgaagggg
     6601 tggccccgat gaggaagccg acgtgtccaa gtgacggatc ggatgtgcgc gagagcacga
     6661 cgatggcgcc gaactcgggt tcatcggtgg caaaacccca gccgagatat gagcgcgcga
     6721 gcagcgagcg cgtcggctcg aggccagaac gcgcgaggca tgcgccgacg aacgcggcac
     6781 accatgcggt ctcgtcgctt gtgatcgccg ggtggcctgc gtcgcggaac atggcgcgga
     6841 tgcgggcgtt ggcgccgctg ccgccgatct cgcgctggcc aagctcggtc caggcctcgg
     6901 caagccaagg cggctggagc atctgtgatg gggtcggtga catgtgcgtg tggagcggag
     6961 gatcagaggg ttgtgaggcg cggcgtgccg cggccagcgg tggcgctcag ctggaagacg
     7021 cggacggtga gcgagggctg gggcgcgccg aaatcggcgg tctgctcggc ggcggtgtag
     7081 ctggcgctcg gcgtcaccgc ggctatggtg cgcttcactg tcgtgccgtc gaggatgtcg
     7141 acctcgtagc gctcgctgtc ctcgccgagg ggcacctcga cctgctccca gttgtcgccg
     7201 ccgatgcgcg tccgccggac ccaggtgagt gcgaggtcgc cactcgctcg ctggccgcgg
     7261 acatggcagg ggctgagggg acggaggccg cgaccgctga aggcatgcgt ctcagtgacg
     7321 tagccgggcg agctcaaggg ttgcgttgcg ggaccatagc gccaggagat cgccaggccg
     7381 acatcggcgg cagagaggtc cacgcgggca acggcggcgt cgagcagcac gaagcgcgcg
     7441 ccggcggaga cgctcgccgg catggcgtcg tcgctgccgg cctgcgcgcg caggaagcgg
     7501 ctcagggagt aggtgagcgg cgcggcgagc gtggccgcgg cgaattgcac gacttcccag
     7561 cgatcgggcg ccgtctcgat cgcagcggcg ttggcgccgg acaggagcgc cgcgtcggtg
     7621 atcgaagcga ggctgccgcg
//
LOCUS       DAORNH010000224         7606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7133_length_7606_cov_2.824262, whole genome shotgun sequence.
ACCESSION   DAORNH010000224 DAORNH010000000
VERSION     DAORNH010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_7606_cov_2.824262"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK264_18705"
     CDS             <1..860
                     /locus_tag="PK264_18705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD77939.1"
                     /translation="MAFESLFKLVAMLALGARDRGATIHQQCAARALDVTDGAVCGVI
                     TERGRIRASRAILAGGAWSSLLSRRHGVPFPQASVRSTVLRTEVAPDGPDAFYCPEIA
                     LTRRLDGSYTIAISGKGTLEVTPQGLRYARQFLPMFLKRFKSVELRLGRSFVSGPEAL
                     ARWSADTISPFERSRVLAPAPDRRAIRQLLSRAQEHFPALRAIGVRESWASYIDSTPD
                     AVPVISTVAGLDGLVLAAGFSGHGFGIGPGAGHLAADIALGRSPIVDPKPFRHARLID
                     GSRGEVGEF"
     gene            complement(1679..>1846)
                     /locus_tag="PK264_18710"
     CDS             complement(1679..>1846)
                     /locus_tag="PK264_18710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HRD77940.1"
                     /translation="GGSDGNFTGALGVPTLDGLGVLGDGPHTLDEHLLVSCLVPRTKL
                     LARLFETLGAA"
     assembly_gap    1847..1867
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1868..2824)
                     /locus_tag="PK264_18715"
     CDS             complement(<1868..2824)
                     /locus_tag="PK264_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRD77941.1"
                     /translation="MIRLTTEELLSEVRAWVEIESPTNCKAGVDRVADLVEAKLRALG
                     AAIERTAGVDGFGDILIGRIGGRTQVAGILLLGHMDTVHQVGTLAGPLPFRVEGDRAY
                     GPGIYDMKGGNAIALAALAHLDAQDRRPHLPVTVMMIPDEEVGSPTSRAAIEREARRH
                     AIALVVEPSGDGGRLTVGRHGIARWHLKTVGRSAHAGAYHGKGRSAIREMARQILAIE
                     ALTDYSRNFFVNIGTVSGGTHENMVPAECRAICYALVPTAREETELREALMALRPHDP
                     DVALEVTAGLSRPPYGKSPAIQALYDQARRLAETIGYPVKGER"
     gene            complement(2913..4868)
                     /locus_tag="PK264_18720"
     CDS             complement(2913..4868)
                     /locus_tag="PK264_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HRD77942.1"
                     /translation="MGALTVPRIFTIGVLCLLLAILHAVAPAVAGPADFAIIIGNGRY
                     KNGVPEVKYAHNDMTAVAKAAEDVFAIPAANVRAVPDISLARFNELFGSSYVTPAGRQ
                     SFLESIVSDETKVVLIYYSGHGVPGVTGGKSRPHLLPIGSDPMAAAVSAYALDDLIAA
                     TRAVLERKAPKARAIIIVDACFSGTSDGGTLTPGTSGTAFSIDFNALMNDRVTVITAA
                     GGAEVAYWDTDRKHGLFTSALLDGLYGAADKRGDGGDGDFQLTIKELQSFTTARMTTR
                     LSALYPREFKRQSPTFPSTSAAVLVTFEGRYPERRLMGREQEERQCRFFEAFGDKQSP
                     AVLEQFLATCGGGCRCRSAIEALLKRHTAKPSTCEADRNIWELARTSRDLVQLQFIAD
                     HSGCPEFRAAAEAMMADARGKAKAKEDQIAAEKARIEREQRESERLAEERRKLAEKLA
                     EAERLRLKVETDMIEERRKLAAAAADAARTPPPSYVMSRLTGRYVEGDGDLDASFASS
                     YAECTARCISNDRCFGIEFYKPKMQCNLYSSARWDPRPSSDGLAEVAVKRAASSTVAS
                     MPVPTYSPPVASAAVTRIPRAYFKEDSTWRTAGTSFEDCTRQCEAGAGCVAFEFYRPR
                     RQCILFRFLPTVVRDAGSNAEIGQLNR"
     regulatory      5045..5266
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5495..>6771
                     /locus_tag="PK264_18725"
     CDS             5495..>6771
                     /locus_tag="PK264_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRD77943.1"
                     /translation="MPFHLIAGRISPITSLAALAAVTIWLPSAHAQTTLPGVVIDSGS
                     IAVPPVKKPAPPADPTPAPATTPKASAAKSKAAPSSAPASGPTVDAASPAGTSSSSDG
                     TVITADRTGASVSVVTGDELRRQQIRHAADALRSLPGVSVGRTGGFGGLTQVRIRGAE
                     GNHTLVLIDGVEANPAVTGEFDFSNLGTDDIERIEVLRGPMSGLYGTGAIGGVINIVT
                     RSGKGPLTLRARAETGAFNTTDMAASASGGSERAHGAVSVSRRSTNGFNISPSGGEDD
                     PAAITTLSAKGGVMMMDGVALDFTLRNVRRTGGRDNDGGIAGQLATQVDTPSHFATST
                     FLAGLNLRVDTFDGRLTHRLYANTSRSTLDDTDVSAFGAFFARNQSSSERFGYASTLR
                     LDMPALFGSRHFLTGLVEREFEAFNAVTADGIER"
     assembly_gap    6772..6803
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6804..>7606
                     /locus_tag="PK264_18730"
     CDS             <6804..>7606
                     /locus_tag="PK264_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD77944.1"
                     /translation="SYAFEWRGEFAERVFLSASVREDDNEVFGAFTTWRAASSVWLYR
                     SGSVGIRPHASVGTGVKVPTMFEQFGIIPGFFTPNPSLRPETSFGWDGGVELSGFHGR
                     AILDVTYFNQTLEDRIETVFAPVWTAVNRPGDSHREGVEVAARFRPFDAFMLGLSYTY
                     LDATYPDGAREIRRAPHSGRVDAGYLFDGGRGSISVAAIYNGRMDDAAFRLPFFTTER
                     VALSDYWLVTAGASYKLQPGLEIYGRVENLLDQRYQEVYGFNTPGIAAY"
BASE COUNT         1203 a         2630 c         2381 g         1339 t
ORIGIN      
        1 cgatggcgtt tgagtcgctg ttcaagctgg tcgccatgct cgccctcggc gcccgcgacc
       61 gcggagcgac gatccatcag cagtgtgcgg cgcgcgcact cgatgtcacg gacggcgccg
      121 tgtgcggtgt catcacggag cgggggcgca tccgtgcctc gcgcgcgatc ctcgccggcg
      181 gggcctggtc gtcgctgctg agccgccgcc atggcgtccc gtttccgcag gcgagcgtgc
      241 gctcgaccgt cctgcgcacg gaggtggcgc ccgacggccc cgatgccttc tactgtcccg
      301 agatcgcgct cacccgccgg ctcgatggca gctacacgat cgccatcagc ggcaaaggca
      361 ccctcgaggt gacgccgcag gggctgcgct atgcccgcca gttcctgccg atgtttctga
      421 agcgcttcaa atcggtggag ctgcgcctcg gcagatcgtt cgtctccggc ccggaggcgc
      481 tcgcacgctg gagcgcggat acgatctcgc ccttcgaacg gtcacgcgtt cttgcacctg
      541 cccccgaccg tcgcgcaatc cggcagttgc tgtcgcgggc gcaggagcac tttccggcgc
      601 ttcgcgcgat cggcgtgcgc gagagctggg cgagctacat cgattcgact cccgacgccg
      661 tccccgtgat ctcgaccgtc gcgggcctcg acggcctggt gctggcggcc ggcttcagcg
      721 gccacggttt cggtatcgga ccgggtgctg ggcatctcgc cgccgacatc gccctgggtc
      781 gctcaccgat cgtcgatccg aagccttttc gccatgcgcg tctcatcgat ggttcgcgcg
      841 gcgaggtcgg cgagttctga ggcacgaacc gaatggcgat tgccgcaatg gcgccggttg
      901 cagcattcac caagtctcta gtgtggcggt tcggatgttc gtattcactg cgtggcactc
      961 tcgagatgcg aacttccgaa catagcgcca cactaagctc atgtaattcc tagtgtcctt
     1021 atcgatctcg aagttcgtcc gagttcgctg ctcgcggtcg cgaacttcgc gatccgtaca
     1081 ctaggcaccg gcaaagccgc ttcttctcga agcaattccg ttctagcctc gacgcatgat
     1141 gccgacgaac gcgcgttcgg ctggtttttc acctcagcga tcaagcagag atttctagca
     1201 gatgtgcgca ttcgatcgcg cgtgaacagt gggctcctcg gcccaagaat tggccgctgg
     1261 ggaatgcgat ggcctcgaca cattgttcga catgacttct tgaagcgtgg acggtcatgc
     1321 caatcccgac cgatggactt ctctcgcagc aatggcatct cggcaaatcc actcccggtc
     1381 tcctggatct cagggtgcgg ggtgagtggg atccggcgat gggccgttgc tggtcgtgcc
     1441 cccttccggt cttgcaccac ccccatcccc ggcctttcct ccgtcgatgc ggaagggagc
     1501 agacgcccga tctcaagcca agcggatgga tcgccttcac gaggcaaacc acgctgcgag
     1561 gcgctgctta acccgtcccc gcccccttgc gggcagcggg acaaggttgg ggtgcccgcg
     1621 gcccaacgat cgcgccctga aagcctgccc gcagggctgt ccttttttgt gccggcgctc
     1681 aggctgcccc gagcgtctcg aacagccgtg cgagcagctt ggtgcgcggg acgaggcacg
     1741 agacgaggag atgctcgtca agcgtgtgcg gcccgtcgcc gagcacgccg agaccgtcaa
     1801 gcgttggcac gccgagtgcg cccgtgaaat tgccatcgct gccgccnnnn nnnnnnnnnn
     1861 nnnnnnnccg ctcgcccttc accggatagc cgatcgtctc ggcaagcctt ctcgcctgat
     1921 cgtagagcgc ctggatcgcc ggcgacttgc cgtaaggcgg ccgcgacaac cccgccgtca
     1981 cctcgagcgc cacgtctgga tcgtgcgggc ggagcgccat gagcgcctcg cgcagctccg
     2041 tctcctcgcg cgccgtcggc accagcgcgt agcagatggc gcggcactcg gccggcacca
     2101 tgttctcgtg cgtgccgccg ctcaccgtgc cgatgttcac gaagaagttg cgcgaataat
     2161 ccgtgagcgc ctcgatcgcc aggatctggc gcgccatctc gcggatcgcg ctgcgcccct
     2221 tgccgtgata ggcaccggcg tgcgctgagc gaccaaccgt cttcaggtgc caacgcgcaa
     2281 tgccgtgccg gccgaccgtc agccgcccgc catcccctga cggttcgacg acgagggcga
     2341 tcgcgtgccg gcgcgcctcc cgctcgatcg ctgcgcgcga ggtcgggctg ccgacctcct
     2401 cgtccgggat catcatgacg gtcacaggga gatggggccg gcgatcctgc gcatcgagat
     2461 gtgccaaggc ggcgagcgcg atcgcgttgc cgcccttcat gtcgtagatg cccggtccgt
     2521 acgcgcgatc gccctcgacg cggaagggca gcggccccgc aagcgtgccg acctggtgca
     2581 ccgtatccat gtggccgagc agcaggatgc cggcaacttg cgtccgcccg ccgatgcgcc
     2641 cgatcaggat atcgccaaag ccgtcgacgc ctgcagtccg ctcgatcgcc gcaccgagcg
     2701 cccgcagctt cgcctcgacg agatccgcga cgcggtcgac gccggccttg cagttggtcg
     2761 ggctctcgat ctcaacccaa gcgcggacct cgctgagcag ttcttcggtc gtcaacctta
     2821 tcacggtgct tccccgtcca gaccgcggtc tccgcgagac ccgacagcgc aacgatactg
     2881 ccacgtcgag gccttcgtgg gatgaccccg ggtcagcggt tcagctggcc aatttcggca
     2941 ttgctgccgg catcacgcac gacggtcggg aggaacctga agagtatgca ttgccgccgc
     3001 ggcctgtaga actcgaaggc gacgcagccc gcgcccgcct cgcattgacg tgtgcaatcc
     3061 tcgaaggagg tgccggccgt gcgccacgtg ctgtcctcct tgaaataggc gcgcgggatt
     3121 cgcgtgaccg cggcactggc aacgggcgga gaatatgtag gcacgggcat ggacgcgact
     3181 gtgctggacg ccgctctttt cacggcgact tcggcaagtc catcgctcga cggccgcggg
     3241 tcccagcgcg ccgaagagta gagattgcac tgcatcttcg gcttgtagaa ttcgatgccg
     3301 aagcagcggt cgtttgaaat gcagcgcgca gtgcactcgg cgtagctcga ggcaaaactc
     3361 gcatcgagat cgccgtcgcc ttccacatag cggccggtca gccggctcat cacatacgac
     3421 ggcggcggcg tgcgggctgc gtccgctgcc gccgccgcca gcttccgccg ctcctcgatc
     3481 atgtccgtct cgaccttcag ccgcaaccgt tccgcttccg cgagcttctc ggccagcttg
     3541 cgacgctcct cggcaagccg ctcggattcg cgttgctcgc gctcgatgcg ggctttctcg
     3601 gcagcgatct ggtcctcctt cgctttcgcc ttgccgcgtg cgtcggccat catcgcttcc
     3661 gcagccgcgc ggaactccgg acaaccggag tggtcggcaa taaactgcaa ctgaaccaag
     3721 tcgcggctgg tacgcgccaa ttcccaaatg ttgcgatcgg cctcgcaggt cgaaggcttt
     3781 gccgtgtggc gcttcaatag cgcctcgatt gccgaccggc agcgacagcc gccaccgcac
     3841 gtcgcgagga attgctcgag gacagccggc gattgcttgt cgccgaaagc ctcgaagaat
     3901 cgacactgcc gctcttcctg ctcgcgcccc atcaaccggc gctcgggata gcgcccttcg
     3961 aaagtgacca gaacagcagc gctcgtgctc gggaaggtcg gcgactggcg cttgaactcc
     4021 ctcgggtaga gcgcactgag gcgcgtcgtc atgcgcgcgg tggtgaagct ctgaagctcc
     4081 ttgatcgtca gctggaaatc gccgtccccg ccatcgccgc gcttatccgc ggcgccatag
     4141 agcccatcga gcagcgctga tgtgaatagc ccatgcttgc ggtctgtgtc ccagtacgcc
     4201 acctctgccc cgcccgcggc cgtgatcacg gtcacgcggt cgttcatcag cgcgttgaaa
     4261 tcgatcgaga acgcggttcc cgacgtgccc ggcgtcaagg tgccgccatc gctcgtccct
     4321 gagaagcaag catcgacgat gatgatggca cgtgcctttg gcgccttgcg ctccagcacg
     4381 gcgcgtgtcg ccgcaatgag atcgtcgagt gcgtaggcgc tgactgctgc cgccatgggg
     4441 tcggaaccga ttggcagcag atggggcctg gacttgccgc ccgtcacgcc gggcactccg
     4501 tggccgctgt aatagatgag aactaccttt gtctcgtccg agacgatcga ttcgagaaag
     4561 ctctgccgtc cagctggcgt cacataggaa ctgccgaaca actcgttgaa gcgagctagc
     4621 gatatgtccg gcacggcccg gacgtttgca gccggaatgg cgaacacgtc ttccgccgcc
     4681 ttcgccaccg cagtcatgtc gttgtgagcg tacttgacct cgggcacgcc gttcttgtag
     4741 cggccgttgc cgatgatgat cgcaaagtcc gcaggacctg ccacggctgg cgcgaccgca
     4801 tggagtatcg ccaggagcag gcaaagaacg ccgatcgtga aaatgcgcgg caccgtcaga
     4861 gcgcccatct ccgcctcccg aaactcaccg caataatgcg acacattggc accgtgcccg
     4921 acgattgcca atcatgagtt gtgctcgctt gagcggattc cggggacacg ccactacgaa
     4981 ttcgcgtctc gcgcgcgcag tgttgctcat ccgctcgatt gacatcgagc cggctctcgg
     5041 cgcacctatg gtccatcgtg tcggttctct ccgcgaggag agtgaaaagg gaacacggga
     5101 cgacatcgcg ttcgcggcgt ctcatccgtg gctgcccccg caactgtaag cggcgagccg
     5161 gctccacatc tgccactgtc catgcgggca tttccgccgg acgggaaggc cggagccagc
     5221 gttcgagccg tgagccagga gacctgccag cacgggtcgc ccatcgaccg tgcggggcgc
     5281 gcggccgagc ggacacgttg tggcgactga gttaggcaca gttggagtct gccgtcgcca
     5341 tgcgtacgtc ctcgagtcga tcgaattctc gcagagcgag tcttgccgtc gcggtgtgag
     5401 tgatcgcacc cggacagctt gcgctcgaga atcgatcgtc tagggacaac ccgatcgctg
     5461 cggccggctc cccacacggg ggagttgtcg acacatgccg ttccacttga tcgccgggcg
     5521 catttcgccg atcacgagcc tcgccgcgct cgccgcggtg actatctggt tgccgtccgc
     5581 gcacgcacag acgacgctgc cgggcgtggt cattgatagc ggctcgattg ccgtgccgcc
     5641 ggtgaagaag ccggcaccgc cggccgatcc aacgcccgcg cccgcgacca ccccgaaagc
     5701 cagcgccgcc aagtccaagg ccgcgccgag cagtgcaccc gcgagcggcc cgaccgtcga
     5761 tgcggcctcg ccggccggca cctcgtcatc gagcgacggc accgtcatta ccgccgatcg
     5821 cacgggcgcc tcggtcagcg tcgtcaccgg ggacgagctc cgacgccagc agatccgtca
     5881 tgcagccgac gccttgcgca gcctccctgg cgtatccgtc ggccggaccg gcggcttcgg
     5941 cgggcttacg caagttcgaa tccgaggtgc tgaaggcaac cacacgctcg tcctcatcga
     6001 cggcgtcgag gccaatcccg ccgtcaccgg cgagttcgac ttctccaatc tcggcaccga
     6061 cgacatcgag cgcatcgagg tcttacgtgg gccgatgagc gggctctatg gcaccggcgc
     6121 gatcggcggc gtgatcaaca tcgtgacacg cagcggcaag ggcccgctga cgctgagagc
     6181 gcgcgccgag acaggcgcat tcaacacgac cgacatggct gcgtccgctt cgggcggcag
     6241 cgagcgggcg cacggcgccg tctcggtcag ccgccgctcg accaacgggt tcaacatttc
     6301 gccgtccggc ggcgaggacg acccggccgc gatcaccacg ctctcggcca agggcggcgt
     6361 gatgatgatg gacggcgtcg cgctcgattt cacgctgcgc aacgtgcgcc gcaccggcgg
     6421 gcgcgacaac gacggcggta tcgcggggca gctcgcgacg caagtcgaca ccccttcgca
     6481 cttcgctacg agcacgttcc tcgccgggct caacctgcgc gtcgacacct tcgacggccg
     6541 gctcacgcac cgcctctatg ccaacacctc gcgctcgacc ctcgacgaca ccgacgtctc
     6601 ggccttcggc gccttcttcg caaggaacca gagctcgtcc gagcgcttcg gctatgcctc
     6661 gacgctgcgt ctcgacatgc ccgcgctgtt cggctcgcgc catttcctga ccggcctcgt
     6721 cgagcgcgag ttcgaggcct tcaacgccgt cacggccgac ggcatcgagc gnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnntctccta cgccttcgag tggcgcggcg agtttgccga
     6841 gcgcgtgttc ctctcggcga gcgtgcgcga ggacgacaac gaggtattcg gtgcctttac
     6901 gacgtggcgg gccgcctcgt ccgtatggct ctatcgctcg ggcagcgtcg gcatccgccc
     6961 gcatgcgagc gtcggcacgg gcgtcaaggt gccgaccatg ttcgagcagt tcggcatcat
     7021 cccgggcttc ttcacgccga acccgagcct tcgccccgag acgtcgttcg gctgggacgg
     7081 cggcgtcgag ctctcgggtt tccacggccg cgcgatcctc gatgtcacct acttcaacca
     7141 gaccctcgag gatcgcatcg agacggtgtt cgcgccagtg tggaccgcgg tcaatcggcc
     7201 gggcgactct catcgcgagg gcgtcgaggt cgcggcgcgc ttccgtccct tcgacgcctt
     7261 catgctcggc ctctcctata cctatctcga cgcgacgtac ccggacggtg cccgcgagat
     7321 ccggcgcgcg ccgcactcgg gccgcgtcga tgccggctac ctgttcgatg gcggccgcgg
     7381 gtcgatcagc gtcgccgcga tctacaatgg gcgcatggac gacgcggcgt tccgcctccc
     7441 cttcttcacg acggagcgcg tggccctttc cgactactgg ctcgtcacgg ccggcgcctc
     7501 ctacaagctc cagcccgggc tcgaaatcta cggacgtgtc gagaatctcc tcgatcagcg
     7561 ctatcaggag gtctacggct tcaacacgcc cgggatcgcg gcctat
//
LOCUS       DAORNH010000225         7520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7240_length_7520_cov_2.711587, whole genome shotgun sequence.
ACCESSION   DAORNH010000225 DAORNH010000000
VERSION     DAORNH010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7240_length_7520_cov_2.711587"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..289)
                     /locus_tag="PK264_18735"
     CDS             complement(155..289)
                     /locus_tag="PK264_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77945.1"
                     /translation="MPTSAGVTVYMFDMTSNTGDAAGDTYSSIDGIIGTNFGGAAATG
                     "
     gene            complement(434..1114)
                     /locus_tag="PK264_18740"
     CDS             complement(434..1114)
                     /locus_tag="PK264_18740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRD77946.1"
                     /translation="MAVGACTSRAGTWVVVIDDGFGYNHSCLSPNCLTSPDFDWDSLS
                     NGRNGDHDFDPFGQVGDAHGTAVNGIIGSDDNGTGAVGVACSAILVGYRTHGFISDAR
                     PQDIRDSIHHAIVTALADIANPSLSLSNSIASVFGAGVDAARFTEFATSIGTAMNSGR
                     GGLGMTIVKSAGNSRDDGPDSGTALDLDDVNADPWTRDTRQVVVAAADQTRTGSCRTI
                     RASAQPTS"
     gene            complement(1234..>1778)
                     /locus_tag="PK264_18745"
     CDS             complement(1234..>1778)
                     /locus_tag="PK264_18745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD77947.1"
                     /translation="QFACATDVARYAYDPEKAKALLKEAGVAPGAEFDYYGYREREYS
                     EAILGDLTKAGLKPKLQWMQFTAFQQANRKAQTPVAHGTWGSNSIPDVSAIVAHFFTG
                     GPDDLVKDPVVIKMIGEADAQTDPVKRAALWKDVLKKIADEAYWVPLFTYAKYYAFSK
                     DLDFTPTSDEIPPFYAAKWK"
     assembly_gap    1779..1788
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1789..2794)
                     /locus_tag="PK264_18750"
     CDS             complement(<1789..2794)
                     /locus_tag="PK264_18750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD77948.1"
                     /translation="MHSLARCLGLAAALAGVLALAGPGAPAEAGKKTDTLVWATDREN
                     PIADPYFINTRELVVIGHHVWDTLVTTDPKTGEIKPLLATKWSWANATTLELELRKGV
                     KFHSGAEMTADDVVYTLNFVSNKDHAIMNYALLAWIKSAEKIDAHKVRINLHKPFPPA
                     LAYLAGLGFIMQKGHYDNAPTKADGKRDYGAVKANGTGPYMITELKPGDYIHMVRFKG
                     YFTDGPKGDPKIGAIRFRTIKDANTRVAELMTGGVDWLWDVPKDQAERIKSNPAMIVE
                     NAKTLRVAYLAFDVHGASGNPLFKDKRVRMAVAHAINRDAIAKNLVGPASVTIHSACH
                     P"
     gene            complement(2956..3279)
                     /gene="soxZ"
                     /locus_tag="PK264_18755"
     CDS             complement(2956..3279)
                     /gene="soxZ"
                     /locus_tag="PK264_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HRD77949.1"
                     /translation="MSVKPRIKVGDVKAPGDVVEVKTVIQHVMETGQRRDGAGKLVPR
                     NIIHTFSASFAGKQIFKAELQPGISANPFISFFVKIPAAGDMELVWEGDENLKVVEKV
                     PVKIG"
     gene            complement(3297..3788)
                     /gene="soxY"
                     /locus_tag="PK264_18760"
     CDS             complement(3297..3788)
                     /gene="soxY"
                     /locus_tag="PK264_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HRD77950.1"
                     /translation="MVRRSAGGKVSVIGRRDVLLGAGAATLVAALLSGAKGAAAQDKP
                     NGWEDIVKKLMGDAKPVDGKIAIDLPEIAESGNTVPFTVTVDSPMTDASHVKSVSILS
                     TKNPSPLIGTFHFAPEAGKASITSRMRLAGTQDVVTLAAMSDGRFIMSKRMIKVTIGG
                     CGG"
     gene            3996..4406
                     /locus_tag="PK264_18765"
     CDS             3996..4406
                     /locus_tag="PK264_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77951.1"
                     /translation="MLRLDRRGFLGLSLLVTMFTMAAGAPVRLPAAEPDLSRGRYLLM
                     AEEPGCPYCARFHREIGEAYRNSPQGTFAPLVRRPMGHPDLARLKTPARYSPTFILLV
                     DGKEVGRITGYPGSDFFWPIADALLAKAGFKPEE"
     gene            complement(4390..4632)
                     /locus_tag="PK264_18770"
     CDS             complement(4390..4632)
                     /locus_tag="PK264_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77952.1"
                     /translation="MIPAVEALVHDLVEWVAREPRRYEDVMERWRTSCPRLPVWEDAV
                     DRGLVERRIGDDDALYVVATSAGRALIGRDAVTPRA"
     gene            complement(4646..>4885)
                     /locus_tag="PK264_18775"
     CDS             complement(4646..>4885)
                     /locus_tag="PK264_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD77953.1"
                     /translation="LGHSAKLLYFGGLIEQAATLDPLVMGLVVAASMVGTFAARPVLE
                     MLSEQRYRSWTVKLITAIACGYLLQGAWLLANGRV"
     assembly_gap    4886..5028
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5029..5408)
                     /locus_tag="PK264_18780"
     CDS             complement(<5029..5408)
                     /locus_tag="PK264_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HRD77954.1"
                     /translation="MSSTVLIGLLAVMVGTSFLSGIFGMAGGMILMGILLWMFPVPEA
                     MALHAVAQIASNGWRGLLWIRFVRWRAVAAYAAGCLLALLAWSLVRYVPAKAHALIAL
                     GILPFMVQALPKDMTPDPERPADGT"
     gene            5504..6442
                     /locus_tag="PK264_18785"
     CDS             5504..6442
                     /locus_tag="PK264_18785"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD77955.1"
                     /translation="MMEFQQLRTFERIAAAEGVTRAAALLNLSQPAVTRQMQALEAEL
                     GLTLFRRRGRSVQLTEHGADLLVRVRAILADVAALRDRAEALGTGRSGRLTVGATPPM
                     IEGLLAAFLPVFKAEHPEVEIEIIEDGGARLADLVDRGEVTIAYVPAGDDRLEGRLLY
                     PVHVCAVLGRGHELAREASLDIARLADAPLLLLRRGFASRDWFDRACFIAGVYPRVAL
                     ESASHGVVLNLVRAGYGVGVLPSAIASATPGLKLVPLTRAGQGIGRWTMLAWKSGRVL
                     PSYVQLFVDALAGYARLRFPGRSIVRATVTIPKPGP"
     gene            6838..>7101
                     /locus_tag="PK264_18790"
     CDS             6838..>7101
                     /locus_tag="PK264_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77956.1"
                     /translation="MSLPRRSVLALGIGAGAAPLLASAADATPGGRRIATVPHGPVID
                     AGLAPNAEHDQAPALQALIDRLTPHGGRIWIAAGAYRLSRITLR"
     assembly_gap    7102..7133
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7134..>7520
                     /locus_tag="PK264_18795"
     CDS             <7134..>7520
                     /locus_tag="PK264_18795"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRD77957.1"
                     /translation="VVTTGRIEASGAGSIGLEGLVIKGDGLTPGGDQSLVVITECDEL
                     EIARVAISGSKGHGLSLARCRGRITASTISGVAGAGIFSIDALGLEIASNHVHDCANN
                     GILVWRSSPGEDGTIVTANRIERIRAD"
BASE COUNT         1164 a         2351 c         2484 g         1336 t
ORIGIN      
        1 tcgaatcccc gaaggcggag cccgtcacgt gttcgacctc gatgtagacg tcgcctgccg
       61 cctcgactgt gttcagcgcc gcgttggcga gtttttgcgg tgacggctgt cggcgcgaag
      121 tcgtagcgcg cgtggtcgtc gccgaggccg ccgatcagcc cgtcgccgcc gccccgccga
      181 agttcgtgcc gattatgcca tcgatgctcg agtaggtatc gcccgccgcg tcgcccgtgt
      241 tcgacgtcat gtcgaacatg tagacggtga cgccggccga ggtcggcatt ggcgtcgtac
      301 gtgagcgaca cgacaccggt caccagcggc tccgcggccg atgtgccgtt gaaggtcgtg
      361 aagccggtcg acgcgtagcc ggccgcgccg acgcgatctg tcgtgatcac gtcactggtg
      421 gtgccgagcc cggctatgag gttggctgcg ccgaagctcg aatagtacga cacgaacccg
      481 ttctggtctg gtcggcggcg gcaaccacga cctggcgtgt gtcacgcgtc cacgggtcgg
      541 cgttgacgtc gtcgaggtcg agtgcggtgc ccgagtcagg gccgtcgtca cgcgagttgc
      601 cggccgactt gacgatcgtc atgccgagcc cgcctcggcc ggagttcatg gcggtgccga
      661 tcgacgtggc gaactcggtg aagcgggctg catcgacgcc ggcgccgaac acgctggcga
      721 tggagttgga gaggctcagg ctcgggttgg cgatgtcagc cagggccgtc acgatagcgt
      781 gatgtatcga atcgcggatg tcctggggcc gggcgtcaga gatgaagccg tgcgtgcggt
      841 agccgacgag gatggcgctg caggctactc cgacggctcc cgtgccgttg tcgtcagacc
      901 cgatgatacc attcaccgcg gtgccgtggg cgtcgccgac ctggccgaac gggtcgaagt
      961 cgtgatcgcc gttgcggccg ttgctaagcg aatcccagtc gaaatccggc gacgtcaaac
     1021 aattcggaga gaggcaggaa tgattgtagc cgaatccgtc gtcgatcacg acgacccagg
     1081 tgccggcccg gctcgtacag gcaccaacag caatggctta cgcgccccca ccctggccct
     1141 cccccgaaag ggggagggga cgatagaggc gtcaggcccg cgcgaattca ggtctcgggc
     1201 ccgtcctcga gagttgcgtc ggacgcagct cggtcacttc cacttggccg catagaacgg
     1261 cgggatctcg tcggaggtcg gcgtgaagtc gaggtccttc gagaacgcgt aatacttggc
     1321 gtaggtgaag agcggcaccc agtaggcctc gtccgcgatc ttcttcagga cgtccttcca
     1381 gagagcggcg cgcttcacgg gatcggtctg cgcgtcggcc tcgccgatca tcttgatgac
     1441 gacagggtcc ttgacgagat cgtccggccc gcccgtgaag aagtgggcga cgatcgccga
     1501 gacgtcgggg atcgaattcg agccccaggt gccgtgcgcg acaggcgtct gcgccttgcg
     1561 gttcgcctgc tggaatgcgg tgaactgcat ccactgcagc ttcggcttga gcccggcttt
     1621 ggtgaggtcg ccgaggatcg cctcggagta ctcgcgctcg cggtagccgt agtagtcgaa
     1681 ctcggcgccg ggagcgacgc cggcctcctt gaggagcgcc ttcgccttct ccggatcgta
     1741 cgcatagcgc gcgacgtcgg tcgcgcacgc gaactgatnn nnnnnnnncg ggatggcagg
     1801 ccgagtggat cgtcaccgag gccggtccga cgaggttctt cgcgatcgcg tcgcggttga
     1861 tcgcgtgggc aacggccatt cgcacgcgct tgtccttgaa cagcgggttg ccgctcgccc
     1921 catgcacgtc gaaggcgagg taggcgacgc gcaaggtctt cgcgttctcg acgatcatcg
     1981 ccgggttcga cttgatgcgc tcggcctggt ccttcggcac gtcccagagc cagtcgacgc
     2041 cgcccgtcat cagctcggcg acgcgcgtgt tggcatcctt gatggtgcgg aagcggatgg
     2101 cgccgatttt gggatcgccc ttcgggccgt ccgtgaagta gcccttgaag cggaccatat
     2161 ggatgtaatc gccgggcttc agctcggtga tcatgtaggg gccggtgccg ttcgccttca
     2221 cggcgccgta gtcgcgcttg ccgtcggcct tggtcggcgc gttatcgtag tggcccttct
     2281 gcatgatgaa gccgaggccg gcgaggtagg cgagcgccgg cgggaacggc ttgtgcagat
     2341 tgatgcgcac cttgtgggcg tcgatcttct ccgccgattt gatccacgcg agcagcgcgt
     2401 agttcatgat cgcgtggtct ttgttcgaga cgaagttcag cgtgtagacg acgtcgtcgg
     2461 cggtcatttc ggcaccggag tggaacttca ctcccttgcg aagctccaac tcgagcgtgg
     2521 tcgcgttggc ccacgaccac ttggtcgcga gcagcggctt gatctcgccg gtcttcgggt
     2581 cggtcgtaac gagcgtatcc cagacgtggt ggccgatgac cacgagctca cgcgtattga
     2641 tgaagtaggg atcggcgatc ggattctcgc gatcggtcgc ccagacgagg gtatccgtct
     2701 tcttgccggc ctcggccgga gcgccgggac ccgcaagcgc gagcacgccc gctagtgccg
     2761 ccgcgagccc gaggcatcgc gctagactgt gcatcaccct ctcccttgct gatgtgatgt
     2821 cgtgacggcg gcattctaac ggaagagtgc cgccatgcca gtacgttggc cagtgcattg
     2881 gccggacgca tcgcgaacaa acgcattagg gcggacccga gggcccgccc gcatccacgc
     2941 agctcgctgc gtcgatcagc cgatcttcac cgggaccttc tcgacgactt tcaggttttc
     3001 gtcaccttcc cagacgagct ccatgtcgcc cgccgcaggg atcttgacga agaaggagat
     3061 gaagggattg gcggagatgc ctggctgcaa ctcggccttg aagatctgct tgccggcaaa
     3121 gctcgccgag aacgtgtgga tgatattgcg cggcacgagc ttgccggcgc catcgcggcg
     3181 ctggcccgtc tccattacgt gctggatgac ggtcttgacc tcgacgacgt cgccgggggc
     3241 cttcacgtcg ccgaccttga tacggggctt tacggacatg gtgttttctc cctctctcag
     3301 ccgccgcagc cgccgatggt gaccttgatc atgcgcttgc tcatgatgaa gcgcccgtcc
     3361 gacatcgccg ccagcgtgac gacgtcctgc gtgccggcga gccgcatgcg gctcgtgatg
     3421 ctggccttgc cggcttccgg cgcgaagtga aacgtgccga tcagcgggga tgggttcttg
     3481 gtcgacagga tcgacacgga cttcacatgg ctcgcgtccg tcatcgggct gtcgacggtg
     3541 acggtgaagg gcaccgtgtt accgctctcg gcgatctcgg gaagatcgat ggcgatcttg
     3601 ccatcgacgg gcttggcgtc gcccatcagc ttcttgacga tgtcttccca gccgttcggc
     3661 ttgtcctgtg cagccgcgcc cttggcgccg ctcagaaggg ccgcgacgag cgtcgccgca
     3721 cccgcgccaa gcagcacgtc gcgccgcccg atcacactga ccttgccgcc tgcactacgt
     3781 ctgaccattc gcctcgctcc cgatcaccgg cactcacgcg tgccttgtgt agtcttccgt
     3841 ccaggcatgc tgctgtcagc cactagcgcc gaccgatgcc gattttgtga ctttcattcg
     3901 ctcgtgttgg atatcatatc agcaaacacg aatgcaatga tcggcggtgc tccctcgcgc
     3961 actcgtgagc ggtcgcgaca gagagaggcg agtgcatgtt gcgattggac cgtcgtgggt
     4021 tcctcggcct gagcctgctg gtcaccatgt tcaccatggc cgctggcgct cccgtacggc
     4081 tgccggccgc ggagcccgat ttgtcgcgcg ggcgctactt gctgatggcc gaggagccgg
     4141 gctgccccta ttgcgcgcgc tttcatcgcg agatcggcga ggcgtatcgc aactcgccgc
     4201 aggggacgtt cgcgcctctc gtgcgccgcc cgatggggca tcccgacctc gcgcgtctga
     4261 agacgccggc acgctactca ccgaccttca tcctgctggt cgacggcaag gaggtcggcc
     4321 ggatcacggg ctatccgggc tccgacttct tctggccgat cgctgatgcg ctcttggcga
     4381 aggccggctt caagcccgag gagtgacggc atcgcgccca atgagcgccc gacccgcgga
     4441 ggtcgccacg acatagagcg catcatcatc gccaatccgg cgctcgacga gcccgcgatc
     4501 gacggcgtcc tcccagacag gcagtcgcgg gcaagacgtg cgccaccgtt ccatgacgtc
     4561 ctcatagcgg cgcggctcgc gagcgaccca ctcgacgaga tcgtgcacca gggcttcgac
     4621 tgcggggatc atgagctctc tccactcaga cacgtccatt ggccagaagc catgcgcctt
     4681 gcagtaagta gccgcaggcg atggcagtga tgagctttac ggtccaggag cggtagcgct
     4741 gctccgacag catctcgagg accgggcggg cggcgaaagt gccgaccatc gatgcggcga
     4801 cgacgaggcc catgacgagg ggatcgagcg tggcggcctg ctcgatgagg ccgccgaagt
     4861 agaggagctt cgccgagtgg ccgagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt gccatcggcg
     5041 ggccgctccg gatcgggcgt catgtccttg ggcaatgcct gcaccatgaa tggcaggatc
     5101 ccgagggcga tgagtgcgtg cgccttcgcg ggtacgtatc gaacaaggct ccaagccaac
     5161 agcgcgagga ggcagccggc cgcgtaggcg gcgacggccc gccagcggac gaacctgatc
     5221 cagaggaggc cgcgccagcc gttcgaggcg atctgggcga cggcgtgcag tgccatagcc
     5281 tccgggacgg ggaacatcca gagcaggatg cccatcagga tcatgccgcc cgccatgccg
     5341 aaaattccag agaggaacga cgtgccgacc atgacggcaa ggagaccgat cagaaccgtg
     5401 ctgctcatcg tgccgcccaa gactgcccga gtgaagggtc gcgcggttcg acaaatgcgt
     5461 ccaatgcatt tattagtgcc ttatccatcc atttggagca tgaatgatgg aattccagca
     5521 gctgcggacg tttgagcgga tcgctgccgc cgagggtgtc acgcgcgcgg cggcgctcct
     5581 caatctcagc cagccggcgg tgacacggca gatgcaggcg ctcgaggccg agctcggcct
     5641 cacgctcttc aggcgccggg gacgcagcgt gcaactcacg gagcatggcg cggacctgct
     5701 cgtccgggtg agggcaatcc ttgctgacgt tgcggcgttg cgcgatcgcg ccgaggccct
     5761 cggcacgggt cgctcgggcc gtctcacagt gggcgcgacg ccacccatga tcgaggggct
     5821 gctcgccgca ttcctgccgg ttttcaaggc ggagcatcct gaggtcgaga tcgagatcat
     5881 cgaggatggc ggcgcacgtc tcgccgacct cgtcgatcgg ggcgaggtga cgatcgccta
     5941 tgtgccggcc ggtgacgatc gtctcgaggg acgtctcctc tatccggttc acgtctgtgc
     6001 cgtgctcggc cgtggacacg aactggcgcg ggaggcctcg ctggatatcg cccgcctcgc
     6061 cgacgcgccg ctactcctgc tgcgccgcgg gttcgcctcg cgggactggt tcgacagggc
     6121 gtgcttcatc gccggcgtct atccgagggt tgcgctcgag agcgcgtcgc acggcgtcgt
     6181 cctcaacctg gtccgcgcgg gctacggggt cggtgtcctg ccgtcggcga ttgcgtccgc
     6241 gaccccgggt ctgaaactcg tgcccctgac acgcgccggc cagggtatcg gtcgatggac
     6301 catgctcgcc tggaagtccg gccgggtgct tccgtcgtat gttcaattgt tcgtcgacgc
     6361 gctcgccggc tatgcacggc tccgcttccc cgggcgctcg atcgttcgcg cgacggtcac
     6421 gatcccgaaa ccagggccct aaccgggtat ccctggaatg tcagaggtcc aagcgagcct
     6481 tacgggctcg ctaagcgcgt ccggcggcct cgaactatga tgggccgtgg gtatggccat
     6541 ggggtgaaag gctcccatcg ttgcccgctg gttccacgga cattccgccg gcccgttcgc
     6601 gggctccgaa ttccgggcga ctgagaatcg gcgcgctcgc cccacactcg tcatcctcgc
     6661 gaaggcgttg acccagctta cgatacagga ggcgggcagc ggccgttccg gcgtgatccg
     6721 ggtgccggcc gccgccttga ccgtgccccg acgcgaggcc ggcgacgcgg ttcatccatc
     6781 tggaagattg gcgcgaggtt tgcagcgtgc ggtggctcag ccattggagc cggtgccatg
     6841 tcgcttccac gtcgcagtgt actggccctc gggattgggg caggggccgc gccgctgctc
     6901 gcttcggcgg cggatgcaac tccgggaggg cggcgcatcg ccacggtgcc gcacggaccg
     6961 gtgatcgatg caggacttgc gcccaatgct gagcacgacc aggcgccggc tctgcaggcc
     7021 ttaatcgacc gcctcacgcc gcacgggggc cggatatgga tcgccgccgg cgcctaccgg
     7081 ctgagccgca tcaccttgag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtcgtca
     7141 cgacgggcag gatcgaggca agcggggcgg ggtcgatcgg tctcgagggg ctcgtcatca
     7201 agggtgacgg cctcacgccg ggcggcgatc agagcctcgt tgtgatcacg gagtgcgatg
     7261 agctcgagat tgcgcgcgtt gcgatctcgg gcagcaaggg gcatggcctc agtctcgcgc
     7321 gctgccgcgg gcggatcacc gcttcgacca tcagcggggt cgcgggcgcc ggaattttct
     7381 ccattgatgc gctggggctc gagatcgcct ccaatcatgt ccacgactgc gccaacaacg
     7441 gcattctcgt ttggcgctcg agcccgggag aggacggcac gatcgtcacc gcgaaccgca
     7501 tcgagcgcat tcgcgcggac
//
LOCUS       DAORNH010000226         7456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7336_length_7456_cov_2.775571, whole genome shotgun sequence.
ACCESSION   DAORNH010000226 DAORNH010000000
VERSION     DAORNH010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7336_length_7456_cov_2.775571"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PK264_18800"
     CDS             <1..1193
                     /locus_tag="PK264_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77958.1"
                     /translation="FGFSLGKTEFDGQIYGAASAAADCFKALVPFFLFAAIKNRIWSQ
                     AAAAAVVWVVVTAYSLTSALGHAALNRFDTTGQRAVDAQVYKDLRADLKRAQDQLSWI
                     PQHRPAASVQSAIDGLKGQPLWKSSKGCADQNRWSREFCQQYFTLTAELAAGQQAVQL
                     EARIAEISTKLAAVKGGQTMAEADPQAVVLARLAGLALPNVKVEDIQTALTIFVALLL
                     EIGSGFGMYVAFSQWRLYDRQVPAVTETRTTSMPVSTAHVEPIPAPIAVPAATAVAAQ
                     APPVPALAAPAVKRVTANDNKVPVKGIAPETDVERFYKERIETTDGSTVTATELYEDY
                     CAWCEQLNKEPLALPTFGREFGELGVQKQRIGGRTRYIGIALKAAVDREEDKKLTASI
                     SHAA"
     assembly_gap    1415..1487
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1488..1956)
                     /locus_tag="PK264_18805"
     CDS             complement(<1488..1956)
                     /locus_tag="PK264_18805"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRD77959.1"
                     /translation="MVVLNRIYTRTGDDGTTALGTGERLPKFAPRIAAYGTVDETNAQ
                     IGMARIHLGSSDPALDQRLGRIQNDLFDIGADLCVPDRGQPLPYTPLRVSDAQVKRLE
                     EEIDDMNGALPALRSFVLPGGTAAAAALHVARTVCRRAERLVVELAAMEGEPVS"
     gene            complement(2008..2997)
                     /locus_tag="PK264_18810"
     CDS             complement(2008..2997)
                     /locus_tag="PK264_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRD77960.1"
                     /translation="MKDHKSRVAPTIADVLAAPAGLPLGDDLRVAAARWLDTLTLDRG
                     LAEKTREAYERDLRQLIAFLVQHLGHAPCLADLAALDTRAVRAFLAHRRRTGAESRSI
                     ARGLSALRTFFRFLESEGLVNGSPLARVGSPKLPRAIPKPLTVEKAAAVVAPGECGDW
                     ITARDQAVLLLLYGAGLRISEALSLTPSAAPGHGRDVIRIAGKGGKERMVPILPVIQE
                     AIARYIALCPFPLERDAPLFIGAKGGPLSPRVIQLTVERLRAALGLPETATPHALRHS
                     FATHLLASGADLRQIQELLGHASLRTTQIYTEVDRERLLKVYDAAHPRAGTVT"
     gene            complement(3097..3726)
                     /gene="msrA"
                     /locus_tag="PK264_18815"
     CDS             complement(3097..3726)
                     /gene="msrA"
                     /locus_tag="PK264_18815"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRD77961.1"
                     /translation="MLRRFAAALAVLVAMAAIYPVPAQQSPAPQPAAGQKLATFAGGC
                     FWCMEHPFDKLDGVIATTSGFMGGTLANPTYKQVVNGGTGHKEVVQVIYDPSKVSYER
                     LLEVYWMNVDPYDAEGQFCDRGDMYGTAIFAHDAEQQRLAEAAKAKLAKSGPIKQPIV
                     TEVRAAGPFTAAEDYHQDYYKKNPIRYGYYRAGCGRDARLLAIWGKAPS"
     gene            complement(3736..4158)
                     /gene="msrB"
                     /locus_tag="PK264_18820"
     CDS             complement(3736..4158)
                     /gene="msrB"
                     /locus_tag="PK264_18820"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRD77962.1"
                     /translation="MAADPSKTFEVTKTDEEWQRLLSKEAFYVLRKHGTERAFTSPLD
                     KTYSAGMYNCAGCDLPLFASDTKYDSGTGWPSFWKPLDNAIGTKTDRSFFMTRVEVHC
                     RRCGGHLGHVFEDGPPPTGLRYCMNGVSLKFIPAAAKG"
     gene            4369..4848
                     /locus_tag="PK264_18825"
     CDS             4369..4848
                     /locus_tag="PK264_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRD77963.1"
                     /translation="MLLSALLALGWAIAPPASGVEPDEVLANPVLEARARRLSSGLRC
                     FVCQNQSIDESNAPLARDLRRVVRERLTAGDSDDQVMAFIVARYGDYALLKPRFGLNT
                     LLLWSLPGLLLLGLGLMLWRQARATSVAAPAPLTGDEEARLAEVLASRRAAEEQPKR"
     gene            4960..6513
                     /locus_tag="PK264_18830"
     CDS             4960..6513
                     /locus_tag="PK264_18830"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HRD77964.1"
                     /translation="MNVLPLNFKALRAPRFLAGVLASGLIATAALHPALAQRGPGGAG
                     PAPGIDTPFGRAPLTFADLVEKVRPAVVSISVSSGTARTAPRSNGSRGNPSFPDIPED
                     HPLYEFFKQLPKEFRGPGGPGGPGGQRAQAQGSGFVISEDGYVVTNEHVIKGAGRIQV
                     SFDGDNKFDAELVGADPRTDLALLKIKANQRFPYVRLATKSPRVGDWVLAVGNPFGFG
                     GTVTAGIVSALGREVGAGPYEFMQIDAAVNKGNSGGPTFNLEGEVVGVNTAIYSPSGG
                     NVGIAFAIPAKTVQDVISQLLKTGSVARGWLGVAIGNVDEDTAESLGLKTNGGPPRGA
                     LIGDITAGGPAEAAGLKRNDIVLSVNGQVIKNSRELAQRVAEIAPGTTVAVEIIRDGR
                     PQTVQVRLGTFPGTGPVASKTEPKRSPGTTKVDLLGLTLAPLSGSGREGVAITEVNPD
                     SHAASRGLKAGDVILEINNQPVSSIGDVESGIKRATDAGRPAVMLRVKTAEGMMQVPV
                     RLEKTARRD"
     gene            6610..7290
                     /locus_tag="PK264_18835"
     CDS             6610..7290
                     /locus_tag="PK264_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD77965.1"
                     /translation="MRILVIEDDRDIAHYLQKSLREAGHSTDLAADGETGAQLASAGT
                     YDVLIVDRMLPRRDGLAVIEDLRRQGNRTPVLVLSTLNSVDDKVKGLKAGGDDYLAKP
                     YDTQELLARIEALARRVAGDDGRTRYVVGDLVLDRLSHKVTRGGTSIVLQPREFRLLE
                     FLMKHAGQVVTRTMLLEGVWDYHFDPQTNVIDVHISRLRAKIDKEFDRPLLHTVRGTG
                     YVVRDGDA"
BASE COUNT         1216 a         2430 c         2491 g         1246 t
ORIGIN      
        1 gctttggttt cagtctcggg aagacagagt tcgacgggca gatctatggc gcggcaagtg
       61 ccgcggcgga ctgcttcaag gcgctcgtgc cattctttct cttcgccgcg atcaagaata
      121 gaatctggtc gcaagccgct gcagcagcgg tcgtgtgggt ggtcgtcacg gcctactcgc
      181 tcacctcggc actcggccat gcggccctca accggttcga tacgaccggc cagcgggcgg
      241 tcgacgcgca ggtctacaag gaccttcgtg cagacctcaa gcgcgcgcag gaccagctga
      301 gctggatccc gcagcatcgc ccggcagcgt ccgtgcagag cgcgatcgac ggcctcaagg
      361 gccagccgct ctggaagtcg agcaagggct gcgctgatca gaatcggtgg agccgagagt
      421 tctgccagca gtacttcacg ctgacagccg agctggccgc agggcagcag gcagtccaac
      481 tcgaggcccg catcgcggag atctcgacga agctcgccgc cgtgaagggc ggccagacga
      541 tggccgaggc cgatccccag gcagttgtcc tggcgcgcct cgccggcctc gcattgccga
      601 acgtgaaggt cgaggacatc caaactgcgc tcacgatttt cgtggcgctc ctgctcgaaa
      661 tcgggtcggg gtttggcatg tacgtcgcct tcagccagtg gcgcctgtat gaccgccagg
      721 tgcccgccgt caccgagacc aggacgacgt ccatgccagt ctcgacggct cacgtggagc
      781 ctatccctgc tcccatcgcg gttcccgcag ccactgctgt cgcagcccag gcgccacccg
      841 tgccggctct cgcagcacct gccgtgaaac gggtcacggc caacgacaac aaggtgccgg
      901 tcaaaggtat cgcgcccgaa actgacgtcg aacggttcta caaagagagg atcgagacta
      961 cagatgggtc tactgtcact gcgactgagc tctacgagga ctactgcgcg tggtgtgagc
     1021 agctgaacaa ggagcctctt gcactgccga cgtttggacg tgaattcggt gagcttggag
     1081 ttcagaagca gaggattggc ggccgcaccc gatacatcgg gatcgcactc aaggccgcgg
     1141 tagaccgcga ggaggataag aagctgacag cttcgatatc ccacgcggct tgaaggccgc
     1201 accgacagca aagtcggaaa cgcaaaaggg tcgcttcggc ggccctttct gcgtttctga
     1261 gggcgcaatt cgacccgatg ccgggcacct caagccgtgc agcgaggaaa tttcggcagc
     1321 cggttggcat cgccgtcttc gtgcgcgccg ccgccccgcc aaccccgcgc gcctttcagc
     1381 ggttctggcc cggcacccag agcacatcgc caccnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggc tcaccggctc
     1501 gccctccatg gcggccagct cgacgacgag acgctcggcg cgacggcaga ccgttcgcgc
     1561 cacgtggagg gccgccgcgg ccgccgtgcc gccagggagc acgaatgagc gcagcgccgg
     1621 cagcgcgccg ttcatgtcgt cgatctcctc ctcgagccgc ttcacctgag cgtcactcac
     1681 gcgcagcggc gtatagggga gcggctggcc gcgatccgga acgcagaggt ccgccccgat
     1741 atcgaagaga tcgttctgga tgcgcccgag gcgctggtcg agcgccggat cgctgctgcc
     1801 gagatgaatg cgcgccatgc cgatctgggc attggtctcg tcgacggttc cgtaagcggc
     1861 gatgcggggt gcgaacttcg gcagccgctc gccggtgccg agcgcggtgg tgccgtcatc
     1921 gcccgtgcgc gtgtagatcc ggttgagcac gaccatcagg gcctccgcgg taatgtttgc
     1981 cgcaaggtag ggccctgttg cgtttggtca agtcactgtg cccgcgcggg gatgggccgc
     2041 atcgtagacc ttgagaagcc gctcgcggtc gacctcggtg tatatttgtg tcgtccgcag
     2101 cgacgcgtgc ccgagcaact cctggatctg gcggagatcg gcacccgagg cgagaaggtg
     2161 cgtcgcaaaa gagtggcgga gcgcgtgcgg cgtcgccgtc tcgggaaggc cgagtgccgc
     2221 ccgcaggcgt tcgacggtga gctgaatgac ccgcggggag agcggaccgc ccttggcacc
     2281 gatgaagagc ggcgcgtcac gctcgagcgg gaaggggcat agggcaatgt agcgcgcgat
     2341 cgcctcctgg atcaccggca ggatcggcac catgcgctcc ttgccgccct tgccggcgat
     2401 gcggatcacg tcgcggccat gaccgggcgc cgcagacggc gtgaggctca aggcctccga
     2461 gatgcgcagg cctgcgccat agaggagcag aagcacggcc tgatcacgtg ccgtgatcca
     2521 gtcgccgcac tctcccggcg cgacgacggc ggccgccttc tcgacggtga gcggcttcgg
     2581 tatggcgcgg ggcagctttg gcgagccgac gcgggcgagc ggcgatccat tgacaaggcc
     2641 ttcgctctcg agaaagcgga agaacgtgcg caatgccgaa agcccgcgcg cgatggagcg
     2701 gctttccgcg cccgtccggc ggcggtgagc gaggaaggcc cggacggcac gtgtatcgag
     2761 tgctgcgagg tcggcgagac agggcgcatg gcccaggtgc tggacgagaa aggcgatgag
     2821 ctggcggagg tcgcgctcgt aggcctcgcg tgttttctcg gcgagaccgc gatcgagcgt
     2881 cagcgtgtcc agccaacggg cagctgcgac gcgcaggtca tcgccgagcg gcaggcctgc
     2941 gggagcagca agcacgtcgg cgatggtcgg cgccacccgc gatttgtggt ccttcatgac
     3001 tgttagcgcc ccggagtgaa tcagatctcg ctccggagcg tcacgttcgc aaggttaacg
     3061 ctaggttaag gcgatcgtcc acggaggcga cttgccttag ctcggcgcct tgccccagat
     3121 cgccagcagg cgggcatcgc ggccgcagcc cgcacggtag tacccgtacc ggatcgggtt
     3181 cttcttgtaa tagtcctggt gatagtcctc ggccgctgta aagggtcccg cagcgcgcac
     3241 ctcggtcacg atcggctgct tgatggggcc gctcttggca agcttcgcct tggccgcctc
     3301 ggcgaggcgc tgctgctcgg cgtcatgcgc gaagattgcc gttccgtaca tgtcgccgcg
     3361 gtcgcagaac tggccctcgg cgtcgtaggg gtcgacattc atccagtaga cctcgagaag
     3421 gcgctcgtag ctcaccttcg acgggtcgta gatgacctgc acgacctcct tgtggccggt
     3481 tccgccattc acgacctgct tgtaggtggg gttcgcgagt gtgccgccca tgaagcccga
     3541 ggtcgtcgcg atgacgccgt cgagcttgtc gaagggatgc tccatgcacc agaagcagcc
     3601 gcccgcgaag gtcgcgagct tctggccggc ggcaggttgc ggcgccggag actgttgtgc
     3661 cggaaccgga tagatcgccg ccatcgcaac gaggaccgcg agcgcggctg caaatcgacg
     3721 tagcatggtc tgtcctcagc ctttggccgc agccgggatg aacttgagcg agacgccgtt
     3781 catgcaatag cgcaggcccg tcggaggcgg gccgtcctcg aacacgtgcc caaggtggcc
     3841 gccgcagcgc cggcagtgca cctcgacgcg cgtcatgaag aagctgcggt ccgtcttggt
     3901 gccgatcgca ttgtcgagcg gcttccagaa gctcggccag cctgtgccgc tgtcgtactt
     3961 cgtgtccgac gcgaagagcg gcaggtcgca tcctgcacaa ttgtacatgc ccgccgagta
     4021 ggtcttgtcg agcgggctcg tgaaggcgcg ctcggtgccg tgcttgcgca gcacgtagaa
     4081 ggcttccttg gaaaggaggc gctgccactc ctcgtctgtc tttgtaacct cgaacgtctt
     4141 cgacggatcc gcggccatgg cggtggctcc tgctcctgtg gcggcggcca atcccgccag
     4201 ggtgaatgcg cggcgcgtga acatcggtgg ctcccttgca gtccatgtgc gctgtcgtgt
     4261 tatacgcaac ggacgacgca cttgaaaatc aaaggctgat cacgtctgcg ccatcggatg
     4321 ataggtcgat attcaactcg tcggcaacga cgcgcgagcc gcctcggcat gcttctctcc
     4381 gcgctgcttg cgctcggctg ggccatcgct ccccctgcgt cgggtgtcga gcccgacgag
     4441 gtgctggcca atccggtgct cgaagcgcgc gcgcggcgtc tctcctcggg gctcaggtgc
     4501 ttcgtctgcc agaaccagtc gatcgacgaa tccaatgcgc cgctcgcccg cgatctgcgc
     4561 cgcgtcgtcc gcgagcggct gacggccggc gacagtgacg accaggtgat ggcgttcatc
     4621 gtagcgcgct atggcgacta tgctctcctg aagccgcgct tcggcctcaa cacgctcctg
     4681 ctctggagcc tgccgggcct gttgttgttg ggcctcggcc tcatgctctg gcgacaggct
     4741 cgggcgacga gcgtggccgc acccgccccg ctcacgggcg atgaggaggc gcggctggcc
     4801 gaggtgctgg catcgcgccg ggcagccgag gagcagccga agcgctgagg cttaccaaga
     4861 tttaatcgtc ccgacatgtc gccgtaaggc gggcttcgct acatcgttgc ggatgggtcg
     4921 acggctgcgc gccgggccca cgctaccgga ggagcatcga tgaacgtgtt gccgctgaac
     4981 ttcaaagcat tgcgcgcgcc gcgcttcctg gccggtgtgc tggcctcggg ccttatcgcc
     5041 acggccgccc tgcatccggc actggcgcag cgtggtcccg gaggcgctgg ccccgcgcca
     5101 ggcattgaca cgccgttcgg tcgcgcgccg ctcaccttcg ccgacctcgt cgagaaggtg
     5161 cgcccggccg ttgtcagcat cagcgtctcc tcggggacgg cgcggacagc accacgcagc
     5221 aacggctcgc gcggcaatcc gagcttcccg gacattcccg aggatcaccc gctctacgag
     5281 ttcttcaagc agctgccgaa agagttccgt ggtcccggcg gccccggcgg ccccggtggg
     5341 cagcgtgcgc aggcgcaagg ctccggcttc gtcatctccg aggatggcta cgtcgtcacc
     5401 aatgagcacg tcatcaaggg cgccgggcgg atccaggtct ccttcgacgg cgacaacaag
     5461 ttcgatgccg aactcgtcgg tgccgatccg cgcacggacc tcgcccttct gaagatcaag
     5521 gcgaaccagc gcttccccta cgtgcgcctc gccacgaaat cgccgcgcgt cggcgactgg
     5581 gtgctggccg tcggcaaccc gttcggcttc ggcggcacgg tgacggccgg catcgtctcg
     5641 gcgctcggcc gcgaggtcgg tgcgggtccc tatgagttca tgcagatcga cgccgccgtg
     5701 aacaagggca actcgggcgg cccgaccttc aaccttgagg gcgaggtggt gggcgtcaac
     5761 acggcgatct acagcccctc aggcggcaac gtcggcatcg ccttcgccat tccggcaaag
     5821 accgtgcagg acgtcatctc gcagctcctg aagaccggct cggttgcccg cggctggctc
     5881 ggcgtcgcca tcggcaacgt cgacgaggac accgccgaga gcctcggcct gaagaccaac
     5941 ggcggcccac cacgaggcgc gctcatcggc gacatcacgg ctggtggtcc cgccgaggcg
     6001 gctggcctga agcgcaacga catcgtgctc tccgtcaacg gccaggtgat caagaacagc
     6061 cgcgagctcg ctcagcgcgt cgctgagatc gctccaggga cgactgtcgc cgtcgagatc
     6121 atccgtgatg gccgcccgca gaccgtgcag gtgcgcctcg gcaccttccc ggggacgggc
     6181 ccggtcgcct cgaagaccga gcccaagcgc tcgcccggca cgacgaaggt cgacctgctc
     6241 ggactcacgc ttgcgccatt gtccggcagt ggccgcgagg gcgtcgccat cactgaggtc
     6301 aatcctgaca gccacgcggc gagccgaggt ctcaaggccg gtgacgtgat cctcgagatc
     6361 aacaaccagc cggtcagctc gatcggggac gtcgagagtg gcatcaagcg cgcaacggat
     6421 gccggccggc ccgccgtaat gctccgcgtc aagaccgccg agggcatgat gcaggtgccg
     6481 gtgcggctcg agaagactgc ccggcgcgac tgacggccgc gctcgtgcta ggatcgacag
     6541 atgtttggaa ggcatggctc cggctgtgcc ttccggtgct ttgggcggcc gtggagttcg
     6601 ggctcgggaa tgcgcattct ggtgatcgag gacgatcggg atatcgccca ctaccttcag
     6661 aagtcgctga gggaggccgg gcattcgact gaccttgccg cagacgggga gaccggtgcc
     6721 cagttggcgt cggccggcac ctatgatgtg ctgatcgtcg accgcatgct gccgcggcgc
     6781 gatggcctcg ccgtcatcga ggatctgcgc cgtcagggca accgcacacc cgtcctggta
     6841 ctctccacgc tcaactcggt cgacgacaag gtgaagggcc tgaaggccgg cggcgacgac
     6901 taccttgcca agccctatga cacgcaggag ctgctcgccc gcatcgaggc cctcgcccgc
     6961 cgcgtggctg gcgacgatgg ccgcacccgc tacgtggtcg gcgatctcgt gctcgaccgc
     7021 ctctctcaca aggtgacgcg gggggggacc tcgatcgtcc tgcaaccgcg cgagttccgc
     7081 ctcctcgagt tcctcatgaa gcacgcgggg caggtcgtga cgcgcacgat gctgctcgaa
     7141 ggcgtttggg actatcactt cgacccgcag accaacgtga tcgacgtgca catctcgcgg
     7201 ctccgcgcca agatcgacaa ggagttcgat cgcccgctcc tgcataccgt ccgcggcacc
     7261 ggctacgttg tccgcgacgg tgatgcttaa tttcatgtcg acgacggcgg gtcggctctc
     7321 ggcgacgttg gttgcggtct tcgcgctggc ggttgcggcg atcttcgtcg tgcttgtgtg
     7381 gcagaccaac accatgctga tccgacaggc ggagctcgcg atcggcgccg aggtcacggc
     7441 gttgcgcgag cggctc
//
LOCUS       DAORNH010000227         7456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7337_length_7456_cov_2.438319, whole genome shotgun sequence.
ACCESSION   DAORNH010000227 DAORNH010000000
VERSION     DAORNH010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7337_length_7456_cov_2.438319"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>259
                     /locus_tag="PK264_18840"
     CDS             <1..>259
                     /locus_tag="PK264_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77966.1"
                     /translation="PRRTVMEIEAEAGLGHHWVTLLSIERPVPRHFGDNHGMLPIWVE
                     ANADWRQAGLVFDRQQPAMRAVRLAVIGVPSAEHASRLKAAL"
     assembly_gap    260..306
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <307..499
                     /locus_tag="PK264_18845"
     CDS             <307..499
                     /locus_tag="PK264_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77967.1"
                     /translation="PLRHRFRNGVDFGEIEVWWTPVLQEALVTCELAAASFEVFSRAD
                     HERMVAARVEAMLRRLARG"
     gene            complement(521..1636)
                     /locus_tag="PK264_18850"
     CDS             complement(521..1636)
                     /locus_tag="PK264_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77968.1"
                     /translation="MLHFGDRFRLLLHKAGLGTRASFVKACTDDGVHGNSRTAGALFD
                     RWTAAAYPPLRKTREERESTSGNDANTKIPAIAKLIANRAKLKSERVEQLLTAPYLSV
                     HALVDELEIDFDEARKSGVTDATYAQWEALRQVPPRLQFMTAIERIMRPSDAEARRER
                     VEQLSALLGRYFLYRRHSTKIGVLREVVDVERIHQRTFASGTYYQWDPARPKTPRRIP
                     FDAFYGGSHVACLAGNSYQSHTKLELTSVQFLLGDIDRELYRHPRGSATRLTFLSGML
                     AGGFDNSDVLMATRVLLMVRGRQQQGSPPLATRGVRVALLPKGSREHDAFADYLACDD
                     RKANVLDNRQSYLNTFLSQTHLQNTLFRRKPPAGGGR"
     gene            2320..2499
                     /locus_tag="PK264_18855"
     CDS             2320..2499
                     /locus_tag="PK264_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77969.1"
                     /translation="MSVYRLGMREWFFDWLAGFSRVADRPPQTRTSPNSNDDQTPRPA
                     RRSVEDIPLWVLGGG"
     gene            2743..3036
                     /locus_tag="PK264_18860"
     CDS             2743..3036
                     /locus_tag="PK264_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77970.1"
                     /translation="MTTTIKRLLSIAAAAGAIWLAGRYAVPLGPTPGAALAGGFVDIG
                     ADISDLLVLWEALKRFLDVAAVATNIAKALQTVIGWLRSKSEPPSKPKDPQWT"
     gene            3120..3362
                     /locus_tag="PK264_18865"
     CDS             3120..3362
                     /locus_tag="PK264_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77971.1"
                     /translation="MSDQVPPAPRKFPAPWTVSEIPGGFRVLDAAGVTVAWVYARDDL
                     AAKSNGRAWLTTAEARRIALWIARSPDLAPHDRRSR"
     gene            3494..3712
                     /locus_tag="PK264_18870"
     CDS             3494..3712
                     /locus_tag="PK264_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77972.1"
                     /translation="MSKPTASPLLQRAESTNAVVEHAASDRTVRCETPRTALRGRFVS
                     PTGKLTLFGVQCVEREGPPARRSRSAAE"
     gene            complement(3949..4326)
                     /locus_tag="PK264_18875"
     CDS             complement(3949..4326)
                     /locus_tag="PK264_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD77973.1"
                     /translation="MTEWDRDDNRVGLHGQGREAVARDRVPGTLPDRDKRRAAGSYID
                     FHIARKIAQRRLDMELSRVEVAAAAGCDVARIAEIETRVLRLSVTEMVRIAAALNTPL
                     HYFYEGLADQSRLLLADGQKHPG"
     gene            4665..4985
                     /locus_tag="PK264_18880"
     CDS             4665..4985
                     /locus_tag="PK264_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77974.1"
                     /translation="MDVDRTIVELSRSYMELAAALAGLARAPNERSPNVDEELDRERV
                     TGGYLDQMTEIAEMMICLRARSLEALRSKALVLKDWCDPRTPEIIDALAISLANDLAA
                     LPSG"
     gene            complement(5098..>5333)
                     /locus_tag="PK264_18885"
     CDS             complement(5098..>5333)
                     /locus_tag="PK264_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD77975.1"
                     /translation="LSRKAHVTLARPEPITVRPPVADPVIAALERAHRPLSNDELAAA
                     MGVTKSEASKRVAALAGMVRKERDGRRVAISLH"
     assembly_gap    5334..5349
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5350..6116)
                     /locus_tag="PK264_18890"
     CDS             complement(<5350..6116)
                     /locus_tag="PK264_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77976.1"
                     /translation="MVGRTTGPDEPNSAKPKGPLLAPVLAHRAASPSQPLRKVDMLKL
                     YSPLQFLAMGLGGALLALQTWLLYEHAAHAASLSPALLAAVPVVTVTLAALPILMEHC
                     LRSRSWLKALGLLILFGLLVGYSLPQAIGRAGEARDAKIAEAIASTRPLELVTEELKR
                     TLTRIDDAEREMKAECKSGFGRKCEGWKRTLAERQASAKALRDDIAKLAPPKVGASDT
                     PRIAAVLGISEATVNLYQPLFLPLAMELGVWVLLWIG"
     gene            complement(6152..6394)
                     /locus_tag="PK264_18895"
     CDS             complement(6152..6394)
                     /locus_tag="PK264_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77977.1"
                     /translation="MDATLRIIAETVVAAHVLREASWTGSRYLASFDQATEDACRRAG
                     ITSETARKLIFFLHVSGEGVDVARHMIEASSPEEAA"
     gene            6740..6982
                     /locus_tag="PK264_18900"
     CDS             6740..6982
                     /locus_tag="PK264_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77978.1"
                     /translation="MTVMMSKDEYRGALAILDLKQEEVGHLLGVGQRTARRWASGEIA
                     VPGPIEMHIRVWLERPELLEVVRRIARERDGSEEHL"
BASE COUNT         1303 a         2285 c         2410 g         1395 t
ORIGIN      
        1 ggccgcgccg cacggtcatg gagatcgagg ccgaggccgg gctcggccat cactgggtga
       61 cgctgctctc gatcgagcga ccggtgccgc gccattttgg cgacaaccac ggcatgctgc
      121 ccatctgggt cgaggccaac gccgactggc gccaggcggg gctggtgttc gatcggcagc
      181 agccggccat gcgggccgtg cgcctcgccg taatcggtgt gccctctgcg gagcacgcga
      241 gccgcttgaa ggccgcgctn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnntccc ttgcggcatc gcttccgcaa cggggtcgac ttcggcgaga tcgaggtctg
      361 gtggacgccc gtgctgcagg aggcgctcgt cacctgcgag ctggccgcgg cgagcttcga
      421 ggtgttctct cgcgccgatc acgagcgcat ggtggccgcc cgagtcgagg ccatgttgcg
      481 acggctggcg cgcgggtgag gtggcaatcc tgcgtgtggc tcaacggcct ccgccagccg
      541 gcggttttct gcgaaacagg gtgttctgaa gatgcgtctg gctcaggaag gtgttcaggt
      601 aactctgtct attgtccagc acattagcct tgcggtcatc acacgccaga tagtcggcga
      661 atgcgtcgtg ctcacggctg ccctttggga gaagggctac tcgaacgcca cgtgttgcca
      721 gaggcggact gccctgctgt tggcgccccc tcaccatgag tagaacgcga gtcgccataa
      781 ggacatcgct gttgtcgaag ccgcccgcca gcattcccga gagaaatgtc agcctcgtcg
      841 cggacccgcg tgggtgtcta tacagctccc ggtcgatatc cccgagcagg aactggaccg
      901 aggtcaattc tagcttcgtg tggctctgat atgaattgcc cgccaggcaa gcgacatgcg
      961 acccaccgta gaacgcgtcg aatgggatgc ggcggggcgt tttcggccta gctgggtccc
     1021 attggtagta ggtgccgctc gcaaaggttc tttggtgtat gcgttcgacg tcgacgacct
     1081 ctcgaaggac gccgatcttt gtcgagtgac ggcgatagag aaagtagcgt cccaagagcg
     1141 cactcaactg ctccactctc tcgcgccgcg cttctgcatc ggacggcctc atgatgcgtt
     1201 cgatcgccgt catgaactgc agacgcggcg gaacttggcg gagcgcttcc cactgggcgt
     1261 atgtcgcgtc agtcactccg ctttttcgag cctcgtcgaa gtcgatttcg agctcatcga
     1321 ccagcgcgtg tacgctgagg tacggtgccg tcaagagctg ctcgacgcgc tctgacttga
     1381 gcttggcccg attggcgatg agtttggcga tggcagggat cttggtgttg gcatcgttgc
     1441 cactggtgga ctctcgctcc tccctcgtct tgcgcagcgg ggggtaggcg gccgcggtcc
     1501 atcgatcgaa cagagccccc gcagttcgtg agttgccatg gacaccgtca tccgtgcacg
     1561 ctttgacaaa ggatgcgcgc gtgccgagtc cggccttgtg cagcagcagt cgaaatcggt
     1621 caccgaaatg gagcactgcg gcgtccccct cccgtcagcg aaacatgtgc gcttcgtgtg
     1681 gactccgcta tcgagtctac atgatcgcat ggaatggttc acatgagagc agagcgacgg
     1741 ttgtctgcgc aataggcaat tcgcgagcac ttctggtgcg acgctactcg ctcgtgctta
     1801 ggcggcagcc aatacggcaa cgattcgctc aattcgattc gatttcgagc atgtcgcgct
     1861 agtgatctga gtgagaacaa aaaaaggctc ccgaccatcg actggtcgga agcctttgga
     1921 ccgatgaacg gagcggatgc tcaaatcacc ggccagcacg ggggcgacca taagcatcat
     1981 cgcgacgggc gtcaaggctg ctccacgcgc tcgtgtggac tcgtcgtcgc agctgcagac
     2041 agtgtcgatc tgcgtggtcg atcagtcgag ctaacgtgtt ggtcttcccg agacaaattt
     2101 cctgccttgc ttaattttct ccttgcgacc caggctctga ttcggcagtc tgacaatgag
     2161 gcaacggggc ggaggctcac gccctcggcg accgaatgcc gcgcaacgac gatgcggcac
     2221 gccgtcgagg gttcatcggc cagcacaccc gagaaccacc tgccccttgt ctcgccctgc
     2281 catcgggcac cgatggcgct gagacgacgg agggacgcca tgagcgtcta tcgcctgggc
     2341 atgcgggagt ggttcttcga ttggctggca ggcttcagcc gagtggctga ccggccccct
     2401 caaacccgca cttccccaaa ctcgaacgac gatcaaacgc ctcgtccggc ccgccggagc
     2461 gtcgaggaca tcccgctctg ggtactcggc ggcggataac cgaggccagc gtctcccagg
     2521 gtcgtggcga cacgacttca cggcggccgc ttcttcccga acggcggcca tcgcaggagt
     2581 cgctggtgct ttcggcttca gcgacactcc ggtcgtcgcg cacctggcgc ttcggtcggt
     2641 tggactcgcg tgttccgctg cgggattgca gtcccgcagc ggaaatccgc aacgccggag
     2701 gcgctctcga aagcatcaac ctccggcacc ttggaggacg tcatgactac caccatcaag
     2761 cgcttgctgt ccattgcggc agcggccggc gccatttggc ttgccggccg ctacgcggtt
     2821 cctctcggcc cgacgcccgg tgcggcgttg gcagggggat tcgtcgatat cggagcggac
     2881 atctccgatc tcttggtcct ctgggaggcg ttgaaacgct ttctcgatgt cgccgctgtg
     2941 gcgactaaca tcgcgaaagc gcttcaaacg gtgatcggct ggctgcgctc caaatccgag
     3001 ccgccgtcca agccgaagga tccgcagtgg acgtaagtaa gcgcttcccg gcgatcatgg
     3061 tcgccggggg taacatttga ccgttgagtt ctttttttgt tccatgctac gcgctgggca
     3121 tgtcggatca agtgccgcca gcccctcgca agtttccagc gccctggacg gtttccgaga
     3181 tcccgggcgg tttccgcgtt ctcgatgcgg ccggcgtgac ggttgcatgg gtctatgcgc
     3241 gggacgacct cgccgccaag agcaacggcc gggcctggct cacgacggcc gaggcccgac
     3301 gcatcgcgct ctggatcgcc cgctcgccgg acctcgcgcc acacgaccgc cgcagccggt
     3361 aacgagccgg ctctcgggac ggtttccagc cccggaaacc gtcggcagaa cgggcaccct
     3421 cacggaaact gttgacgcac catcggcatc gagacgatgc ttggcgagtt ttcccgccat
     3481 agtgagacgc gtcatgagca agccgaccgc ctcgccattg ctccaaaggg ccgaaagcac
     3541 gaacgccgtg gtggagcatg cggcgagcga ccgcacagtg cgctgcgaga cgcctcggac
     3601 agcgcttcgt ggacgcttcg tctcgccaac gggcaagctc acgctttttg gcgtccagtg
     3661 cgtcgagcgc gaggggccac cggcgcgccg cagtcgttcg gccgccgagt aatccccgct
     3721 gctggacccg cgcctatgct gcctcgtcca tcctcgagga gagcggaatg cgccagacac
     3781 gagcccgaga gatcattgaa ggccggctgg tactcgtgag ggcgtgcgtg gccacgcgtg
     3841 accaagccta cgccgttacg cgcgatgcgc ttgcacggga tttgatgacg acggcgctcg
     3901 tgcgaattgt gagcgcgctc gacctgcatc cggccatcat cgagtggatc agccagggtg
     3961 cttctggcca tccgccagaa gtagccggct ctggtcggcg agcccctcgt aaaagtagtg
     4021 caacggcgtg ttcagtgcgg cggcgatacg caccatctca gtgaccgaca gcctcagcac
     4081 gcgggtctcg atctccgcaa ttctggccac gtcgcatcct gcagctgcag cgacctcgac
     4141 ccgcgacagt tccatgtcca gtcgacgctg cgcgatcttc cgagcgatgt gaaaatcgat
     4201 atacgacccg gctgcgcgcc gcttgtcccg atcgggcagc gtgcccggca cccggtcgcg
     4261 ggcaacggcc tcgcggcctt ggccgtgcaa accgacccga ttgtcgtcac ggtcccattc
     4321 ggtcactcgg tacttctccg ctgcctgcac atgaaagtcg cgtctcccaa accgcagaat
     4381 cggcgaaaaa ttctgccaga gcaagccacc tattatccga tgcctgattg aatgaccttt
     4441 gccccatgca gcgccaatga aagtgatttt ccaagacaga agctaatccg ttcgcgggct
     4501 attccctaat ctgacgttgt gggtgatgtg tggcaaagtt gtggatagtc gggggcttgc
     4561 ggatgtcagt gcgttctaat tccaagaggc gccaggacaa ttctgggtgc gcggatcaga
     4621 ctgagagtgg cagttcagac gtagttgagc gtgccgaacg ggcgatggac gtcgatcgca
     4681 cgatcgtgga gctttctcgc agctacatgg agctcgccgc tgcattggct ggcctcgcca
     4741 gggcgccgaa cgagcggtcg ccgaacgtgg acgaagaact ggatcgggaa cgcgtcacgg
     4801 gcggttatct cgaccagatg actgagatcg ccgaaatgat gatctgcctg cgcgcacggt
     4861 cactggaggc gttgcgcagc aaggccttgg tcttgaaaga ctggtgcgat ccgaggacgc
     4921 cggaaatcat cgacgccctg gcgatctccc ttgccaacga tctggcggcg ctgccatccg
     4981 ggtgagccgg tccggagatc gcgtccgatc gccatccgaa ggccggatac cggggagaga
     5041 ccgacaaaaa gccccgcggt ggtgccgcgg ggctcgcgca ggtgcgccca gtgattgtca
     5101 gtgcagcgag atggcaactc ggcggccgtc acgctccttt cggaccatgc cggcgagggc
     5161 ggcaaccctc ttcgacgcct cactcttggt cacgcccatc gcggcagcga gttcatcgtt
     5221 gctcaacggg cggtgcgctc gctcgagcgc ggcgatcacc gggtcggcca cgggcggcct
     5281 gacggtgatc ggctccggtc tcgccagcgt cacgtgagcc ttgcgtgaga gcgnnnnnnn
     5341 nnnnnnnnna agccgatcca gagtagtacc cacacaccca attccatcgc caggggcaga
     5401 aacagcggct ggtagaggtt caccgtcgct tccgagatcc cgagcaccgc ggcgatgcgc
     5461 ggtgtgtcgc tcgcgccaac cttcggcggc gcgagtttgg cgatgtcgtc cctcagggct
     5521 ttggcgctgg cctggcgctc ggccaatgtg cgcttccagc cctcgcattt ccggccgaaa
     5581 ccggatttgc attccgcctt catctcccgc tcggcatcgt cgatgcgggt gagagtgcgc
     5641 ttgagctctt ccgtgacgag ctcgagggga cgtgtcgagg cgatggcctc ggcgatcttc
     5701 gcgtcacggg cttcgccggc ccgtccgatc gcttggggga gtgagtatcc gaccagaagc
     5761 ccgaacagga tcaagagccc gagggccttg agccatgacc ggctccgcag gcagtgctcc
     5821 atcaggatgg ggagcgccgc cagcgtgacg gtgacgaccg gcactgcggc gagcagtgcg
     5881 ggcgacaggc tggcggcatg cgcagcgtgc tcgtagagca gccacgtctg caacgccagc
     5941 agggctccgc cgagccccat tgccaaaaac tgcaggggag agtaaagctt gagcatgtcg
     6001 accttcctta gaggttgact tgggctcgct gcccggtgcg ctaacaccgg ggccaacagc
     6061 gggccctttg gtttggctga gttcggctcg tcagggccgg tggtccggcc gaccatcggg
     6121 ccgctcggcg tgagcggccc taggtttcgc cttaggcggc ctcctcgggc gaggaggcct
     6181 cgatcatgtg ccgggcgacg tcgacgcctt ctcctgagac gtgcaggaag aagatgagct
     6241 tgcgggcggt ctcgctggtg atgccggctc ggcggcaggc gtcctcggtc gcttggtcga
     6301 agctcgcgag gtagcgcgat ccggtccagg aggcctcccg aagcacgtgg gcggcgacga
     6361 cggtctcggc gatgatgcga agtgttgcgt ccatgggacg gctccaaggc tggtgggctg
     6421 gcttgcgcca gggccggcgg aattccgacc ggcgggcttg gagactcgtt ggcagagagg
     6481 aattgacgtc actgtcggcg gcggatgcgg tcgaagagtg ctggaccggt ccgcaggagg
     6541 ggtttcggat ggttccctcg gggctccccg gggcctcgca gcgcccgatc atggccgtcc
     6601 atactttcaa tataggccat tttggcctat aggtcaatat gacctaatca cccgagccga
     6661 gatttgtggt cactttttcg tgatgcatag ggcatttcgg cctatggtat ggaccgaatg
     6721 gactgtcgaa agagacaaaa tgaccgtcat gatgtcgaag gacgaatacc gcggggcgct
     6781 ggccattctg gacctcaagc aggaggaggt cggacatctg ctcggcgtcg gccagagaac
     6841 agcgcggcgc tgggcgtccg gtgaaatcgc ggtcccaggc ccgatcgaga tgcacatcag
     6901 ggtgtggttg gagcggcccg agttgctcga agtggtgcgc aggatcgcac gcgagcgaga
     6961 cggcagcgaa gaacacttat agcacaccgc cccgcccaaa acggccccta gacctgcgtg
     7021 atttcaagtt ttctatgtgg catagtgtcg cattggcaaa aatttagaga acgctcgggc
     7081 tacaatgtcg gcttcttggg gtgtcgacgc gcccgctcgg cgacggcaga cgtgccgtgc
     7141 aatgcagaga gagcagactt tggaccggtt gtcgatcaca agacctgaaa gctaagaggt
     7201 atcgacagct catccactag ttggagacga aagttccagt cggcccgcgc ggcgatctct
     7261 gctagaacgc cgagggcggc gaccaacttg gtgccgacgt tccggtcggc ccgcgcggcg
     7321 atctctgcta gaactcatgc tgtacagcgt aaagacaatg cgcggttccg gtcggcccgc
     7381 gcggcgatct ctgctagaac gccggctagc cgatcgaatc acgccgcaag gttccggtcg
     7441 gcccgcgcgg cgatct
//
LOCUS       DAORNH010000228         7453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7347_length_7453_cov_2.783185, whole genome shotgun sequence.
ACCESSION   DAORNH010000228 DAORNH010000000
VERSION     DAORNH010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7347_length_7453_cov_2.783185"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PK264_18905"
     CDS             complement(<1..512)
                     /locus_tag="PK264_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HRD77979.1"
                     /translation="MAAHRVWIEAALNGPWGQARQPGIPSTVEAIVAEGIACASAGAA
                     IIHVHAYDAPGTAQTFDWQVYARIIEGIRAKVDVPVYPSIPSAALAPGGEETSPAARF
                     AHTEELARRGLLELSVVDPGSVNFASFNDVRLGRTGHVYLNPEPDIRYGLALAAKHGF
                     HPGYAIYEPG"
     gene            complement(537..1652)
                     /locus_tag="PK264_18910"
     CDS             complement(537..1652)
                     /locus_tag="PK264_18910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRD77980.1"
                     /translation="MSKSSDAQLKLRPAFAIERNPGAVAVAERTKLLADPGFGRVFTD
                     HMAIIRYTEGKGWHDAKITARKPISLDPAAAVLHYAQEIFEGLKAYKLADGTMAMFRP
                     EANARRFQASARRLAMAELPEELFVESIRQLVRVDRDWFPTVEGGSLYIRPFMFASEV
                     FLGVKPSSEYLYLVILSPVGAYFKSGAPAVSIWVSQNFTRAAPGGTGAAKCGGNYASS
                     LVAQAEASKHGCDQVVFLDAAEHKWFEELGGMNVFFVFNDGTLQTPPLSGTILPGITR
                     DALITLAKTEGLKIREEPYSIDQCKADAKSGRLVESFACGTAAVVTPIGRLVDPSGEV
                     KIGAGGPGQTTTRLKQKLVDIQRGTAPDPHKWVHRID"
     gene            1878..2405
                     /locus_tag="PK264_18915"
     CDS             1878..2405
                     /locus_tag="PK264_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRD77981.1"
                     /translation="MADIIPFKPVGATGAPSTAAAPEAVAPEMRLVELLFFGYRAFTS
                     EPDAILAELDFGRAHHRVLHFVARRPGLRVAELLDILRITKQSLARVLKQLVDEGFVE
                     QRSGVSDRRERRLYATPAGRQLHRRLADLQCERIRAALAAVGPDCADLVARFLSGMIG
                     EEDRSLLLTLLAPPE"
     gene            2501..>3181
                     /locus_tag="PK264_18920"
     CDS             2501..>3181
                     /locus_tag="PK264_18920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD77982.1"
                     /translation="MTTPQPTPSIRKDPLPDNAPHVLVVDDDHRIRDLLVRFLTDNGF
                     RVSPAGDANAARNAMRSIAFDLIVLDVMMPGDSGLVLAKELKSISNVPICMITALADP
                     EDRVSGLEIGVDDYVAKPFEPRELLIRLRNIMKRGQAAPAPKDEIKIGEMSFNVSRGE
                     LRRGSEAIKLTERERELLRQFAQRPGLPIARHELAPDDSTGSERAIDVQINRLRRKIE
                     VDPSNPVYL"
     assembly_gap    3182..3197
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3335..4678
                     /locus_tag="PK264_18925"
     CDS             3335..4678
                     /locus_tag="PK264_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD77983.1"
                     /translation="MRRWWANRGYSLMPKGLYARALLIVITPIVILECVIAFIFMERH
                     WQQVTRRLSEATARDIAALIDVYHSYGLDDDYGRLMEMARDRLALNMQVLPPGELPAA
                     QPRPFFALLDRTLTSEIRRHVKAPFWIDTVGQSRHVEVRVKLDQAVLRFTATRSQTYP
                     SNSHIFLVWMVVTAVILLVVAILFMRNQIKPIQRLADAADAFGKGRAIPDDFKPRGAL
                     EVRKAAEAFIEMRDRIMTHVDQRTTMLAGVSHDLRTVLTRFRLELAMLPPSQGATAMR
                     ADVDEMQHMLEDYLAFAKGDGGELAKPTPVSDLLEEIQSASAIFGTPVHLSVRKRHQK
                     LVVPLKRNAFKRALTNLVANAARYGKRVLIRLEPDRRWLRVEVDDDGPGIPENERENV
                     FRPFYRIDDARRIEKGGNSGLGLAIARDIARSHGGDIELGQSPLGGLRAAIRVPI"
     assembly_gap    4758..4841
                     /estimated_length=84
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4842..5353)
                     /locus_tag="PK264_18930"
     CDS             complement(<4842..5353)
                     /locus_tag="PK264_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HRD77984.1"
                     /translation="MQLVPNPLIIPVGMFIGVLIAAPVGPVNVLCVQRALERGFWGGV
                     AAGIGAVLGDGLIALLAALGVGAVSGAVAHYRSSIQIVGGLALLAFGVRLYFMRPNIV
                     APNPEQEAKTSLRDFAWDIPQTFFLTITNPAAVLGLFAIFGGVSTFVNVDSWIDALTM
                     VAAIVGGSLM"
     gene            complement(5552..6487)
                     /locus_tag="PK264_18935"
     CDS             complement(5552..6487)
                     /locus_tag="PK264_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD77985.1"
                     /translation="MDQIWIPLAVAAAFFQALRLAGLKVANQSISVFAATLVRGLFGL
                     PVMLAYLAAVVAYEAAGATNSLRIPEFNGRFLLMCLLSGLGQYFGTALLVYAFQMRNF
                     AVGTMLVKADVIMTALIGSLLFSERISASGWVAILVTVAGVLIVSAGRLDFAAMRRSG
                     ESWGDLLLGYPTRICLVSAMIYATSYLTLREALLAIEGSPMARSAWSAVGMTVLQLLV
                     IGPWLLVRHRADVMAIGRILPLAVAVGTASALGTIFWFLATAYTNASYVAAVAQVQIA
                     FTLAISSLYFRERIMPLEIVGMAGVLAGIVLFRFA"
     gene            complement(6487..>7453)
                     /locus_tag="PK264_18940"
     CDS             complement(6487..>7453)
                     /locus_tag="PK264_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD77986.1"
                     /translation="MEPLRQRIAGLYRDWYGVAVAPERIVVTCGSSAAFVLAFLAIFD
                     AGDRVALAAPGYPCYRHILTALGCEPVMIDTGPATRWAPTPEAIAEAARGSRAKGLLL
                     ASPANPTGTMLAPDRLAEIIRTCRQQGMWFISDEIYHGLTYGVRQQTALATSDDVIVI
                     NSFSKYFSMTGWRVGWMVVPERLVRPLERLAQNLYISPPAISQVAALGAFDGIDELEA
                     NKRVYAANRELLLDLLPKAGFSEIVPADGAFYLYADVGRFTNDSLAFAKAMLTEAGVA
                     ATPGIDFDPGRGQRFLRFSYSGSTAAMAEAARRLQAWPRLRSSPV"
BASE COUNT         1322 a         2445 c         2437 g         1149 t
ORIGIN      
        1 aagcccggct catagatggc atagcccgga tggaagccgt gcttggctgc gagtgccaag
       61 ccgtagcgga tgtcaggctc agggttcagg tacacatgcc cggtccgccc cagccgcacg
      121 tcattgaacg aggcgaagtt cacgctgccc ggatctacga cggagagctc gagaagtccg
      181 cgccgggcga gctcctcggt atgcgcaaag cgcgccgcgg gtgacgtctc ctcgccgcca
      241 ggcgcgagcg ccgctgacgg gatcgacggg taaaccggga catccacttt ggcgcggata
      301 ccctcgatga tgcgcgcgta gacctgccaa tcgaaggtct gcgccgtgcc gggcgcgtcg
      361 taggcgtgca cgtggatgat cgcggctccc gccgaggcgc aggcgatccc ctcggcgacg
      421 atcgcctcga cggtcgaagg aatgccgggc tggcgcgcct ggccccacgg gccattgagc
      481 gccgcctcga tccatacgcg gtgcgccgcc atgacctgcc tcctcgcgct ttgacgtcag
      541 tcgatccggt gcacccactt gtgcgggtcg ggcgcggtgc cgcgctggat gtcgacgagc
      601 ttctgcttga gacgcgtggt cgtctggccg gggccgccgg cgccgatctt cacctctccc
      661 gagggatcga cgaggcggcc gatcggcgtc accaccgcgg ccgtgccgca ggcgaagctc
      721 tcgacgaggc gaccactctt ggcgtccgcc ttgcactggt cgatcgagta gggctcctcg
      781 cgaatcttga gaccctcggt cttggcgagc gtgatgaggg cgtcgcgcgt gatgccgggg
      841 agaattgtgc cggagagcgg cggcgtctgc agcgtcccgt cgttgaagac gaagaacacg
      901 ttcatgccgc cgagctcctc aaaccacttg tgctcggccg catcgaggaa gacgacctgg
      961 tcgcagccgt gcttggaggc ctcggcctgc gcgacgaggc tcgacgcgta gttgccgccg
     1021 cacttggctg cacccgtgcc acccggtgcc gcgcgcgtga agttctgcga cacccagatc
     1081 gagactgcag gcgcaccgct cttgaagtag gcgccgacgg gcgagaggat gacgaggtag
     1141 agatactccg acgacggctt cacgccgagg aaaacctcgc tcgcgaacat gaaggggcgg
     1201 atgtagagcg agccgccttc gaccgtcggg aaccagtcgc gatcgacgcg cacgagctgg
     1261 cggatcgatt cgacgaacag ctcctcggga agctcggcca tcgcgagacg gcgggcggac
     1321 gcctggaagc gccgcgcgtt ggcctcgggg cggaacatcg ccatggtgcc atcggcgagc
     1381 ttgtaggcct tcaggccctc gaagatctcc tgcgcgtaat ggagaacagc cgccgccgga
     1441 tcgagtgaga tcggcttcct cgccgtgatc ttcgcatcgt gccagccctt cccttccgtg
     1501 tagcggatga tcgccatgtg gtcggtgaag acgcggccga agccggggtc cgcaagcagc
     1561 ttggtgcgct cagctacagc cacggcgccc ggattgcgct cgatcgcaaa tgcggggcgg
     1621 agcttcagct gtgcgtcgct gctcttggac atcgtcgaac tccctcgctg gtaggcctat
     1681 ggtgtccagg ggtggcccag gtcgctcgcc cttcgatctg atggcgatac gtcacctgtg
     1741 gtcggccaat ctggacgggt cgtgatgctc tgcctcaccc ggacggcacg ctgctacttg
     1801 cttggacagc gcagcaacag tgcggggccc cgagtttttc cttgtcaacc gtatcgatcg
     1861 gagccaaata tgtcaacatg gctgacataa ttccattcaa gccggttggc gcaacaggcg
     1921 cgccatccac ggcggcggcg ccggaggcgg ttgcgccgga gatgcgcctc gtcgagctcc
     1981 tgttcttcgg ctaccgcgcc ttcacgagcg agcccgacgc catccttgcc gagctcgact
     2041 tcggccgcgc ccatcaccgg gtcctgcatt tcgtggcgcg ccgacccggc ctgcgggtcg
     2101 ccgaactcct cgacatcctg cgcatcacca agcagagcct tgcgcgcgtc ctcaagcaac
     2161 tcgtcgacga gggctttgtc gagcagcgct cgggcgtctc ggaccggcgc gagcggcggc
     2221 tctatgcgac cccggccggc cgtcagttgc accggcgcct cgccgatctg caatgcgagc
     2281 gcatccgtgc ggcactcgcg gctgtgggtc cagattgcgc ggatttggtc gcgcgcttcc
     2341 tgtcgggtat gatcggcgag gaggatcgct cgctcctgct cactctgctt gcccctccgg
     2401 agtagcgacg gcggagtcga gcgagtgcca ggatgccagc gccacgccga ggctgcgctc
     2461 gcgccccggg caagggccga ccctgactgc gaggtaacac gtgacgacac cgcagccgac
     2521 cccgtcgatc cgcaaggatc ccttgcctga caatgcgccg cacgtgctcg tcgtcgatga
     2581 cgatcaccgc atccgcgatc tgctggtgcg cttcctcaca gacaacggtt ttcgcgtgtc
     2641 gccggccggt gatgccaatg cggcacgcaa cgcgatgcgc tcgatagcct tcgatctcat
     2701 cgtgctcgac gtgatgatgc cgggcgacag cggcctcgtg ctcgccaagg aactgaaatc
     2761 catctcgaac gtgccgatct gcatgatcac cgcgctcgcc gaccctgaag acagggtgag
     2821 cgggctcgag atcggcgtcg acgactacgt cgccaaaccc ttcgagccgc gcgagctgct
     2881 gatccggctc cgcaacatca tgaagcgcgg ccaggctgcc ccggcgccga aggacgagat
     2941 caagatcggc gagatgtcgt tcaatgtgag ccgaggcgag ctcaggcggg gcagcgaggc
     3001 gatcaagctc acggagcgcg agcgcgagct cctccgccag ttcgcacagc gccccggcct
     3061 gccgatcgca cgtcacgagc tggcgccgga cgattcgacc ggcagcgagc gtgcgatcga
     3121 cgtgcagatc aaccggctcc gccgcaagat cgaggtcgat ccgtcgaacc ctgtttatct
     3181 gnnnnnnnnn nnnnnnnaag ggttacatcc tctacttcga ctgatacctc ctcgaccgac
     3241 aggccgcaca aaccgcatgg tcgccgctga cgacaggatc gacgggcaat cgaagccgcc
     3301 gcgcacccgc ggctggcgaa cctatgcacg cgccatgcgc cgctggtggg caaaccgcgg
     3361 ctacagcctg atgccgaagg gcctttatgc ccgggcgctg ctcatcgtga tcacgccgat
     3421 cgtcatcctc gagtgcgtga tcgccttcat attcatggag cggcactggc agcaggtgac
     3481 gcgccgcctc tccgaggcga cagcgcgcga tatcgcggca ctgatcgacg tctaccatag
     3541 ctacggcctc gacgacgact acggccgtct catggagatg gcccgggacc ggctggcgct
     3601 caacatgcag gtgctgccgc cgggcgagtt gccggcagct cagccccgac cattcttcgc
     3661 gctcctcgat cgcactttga cgagcgaaat ccgccgccac gtgaaggcgc cattctggat
     3721 cgacacggtc ggccagtcgc gccacgtcga ggtgcgcgta aagctcgacc aggcggtgct
     3781 gcgcttcacg gcgacccgca gccagaccta cccgtcgaac tcgcacatct tcctcgtctg
     3841 gatggttgtt accgccgtca tcctgttggt cgttgcgatc ctcttcatga ggaaccagat
     3901 caagccgatc cagcggcttg ccgatgccgc ggacgccttc ggcaaggggc gcgcgatccc
     3961 ggacgacttc aagccgcgcg gcgccttgga agtccgcaag gccgcggagg ccttcatcga
     4021 gatgcgcgac cgcattatga cgcacgtcga ccagcgaacg accatgctcg cgggcgtcag
     4081 ccacgacttg cgcaccgtac tgacccgatt ccgcctggag ctcgcgatgc tgccgccgag
     4141 ccagggggcg acggccatgc gcgccgatgt cgacgagatg cagcacatgc tcgaggacta
     4201 cctcgccttc gccaaggggg acggcggcga gcttgcgaag ccgacgcccg tctcggacct
     4261 tctcgaggaa atccagtcgg cgtccgcgat cttcggcacg cccgtgcacc tctccgtgcg
     4321 caaacgccac cagaaactcg tcgtcccgct caagcgcaat gccttcaagc gcgccctcac
     4381 caatctcgtc gccaacgcgg cgcgctacgg caagcgcgtc ctcatccgcc tcgagccgga
     4441 ccggcgctgg ttgcgcgtcg aggtggacga cgacggtccg ggcattcccg agaacgagcg
     4501 cgagaacgtg ttccgcccct tctatcgcat cgacgatgcg cgccgcatcg agaagggcgg
     4561 caacagcggc ttgggcctag ccattgcccg tgacatcgcg cggagccacg gcggcgacat
     4621 cgagctcggc cagagcccgc tcggcggcct cagggccgcg atccgggtgc cgatctgaag
     4681 gggtgaccaa gtcaggtcag attgctacag cagccgcatc gacttcaggg cgatctcgcc
     4741 gacgagcatg acgccgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacatgagg ctgccgccga
     4861 cgatggccgc gaccatggtg agggcgtcga tccaggaatc gacgttcacg aatgtggaga
     4921 cgccgccgaa gatggcgaac aggccgagga ctgcggccgg attggtaatc gtgaggaaga
     4981 aggtctgcgg aatgtcccag gcaaagtcgc gcaggctggt cttcgcctcc tgctcggggt
     5041 tcggggccac gatatttgga cgcatgaagt aaaggcggac gccgaaagcg agcagcgcaa
     5101 gcccgccgac gatctggatc gatgatcgat agtgggcaac ggcgcccgag acagcgccga
     5161 cgccgagggc ggcgaggagc gcaatgaggc catcaccgag caccgcaccg atcccggccg
     5221 cgacaccgcc ccagaagccg cgttcgagcg cccgctgcac gcaaagcacg ttcaccggcc
     5281 cgactggcgc cgcaatcaag accccgatga acatgccgac cggtatgatc agcgggttcg
     5341 gcaccaattg cattcacgac cccgctcgaa gtgtacgacg gcggttcttt cttagggcgc
     5401 tcgacagatc gagcccatgg tccgtcgcac cggtcgccag tcgcgccgag ctacggccat
     5461 ttgctcgaac aacgggcgct gaagcatgtt ccgccgcacc cgactattcc tagttgcgat
     5521 gcgtgccgct gtccatggct cggcatacga cttaagcaaa gcggaaaagc acgatgccgg
     5581 cgaggacgcc ggccatgccg acgatctcga gcggcatgat ccgctcccga aaatagagtg
     5641 acgagatcgc cagcgtgaag gcgatctgca cttgcgcgac cgctgcgaca tacgaggcgt
     5701 tcgtgtaggc cgtcgccagg aaccagaaga tcgtgccgag agcgcttgcc gtcccgaccg
     5761 cgacagcgag cggcaatatg cgcccgatcg ccatgacgtc ggcgcgatga cgcacgagga
     5821 gccagggtcc gatgacgagc aactggagga ccgtcatgcc gacggccgac caggcgctgc
     5881 gcgccatcgg gcttccctcg attgcgagca aggcctcgcg caaggtcagg taggaggtcg
     5941 cgtagatcat cgccgagacg aggcaaatcc gcgtcgggta gccgagcagc aggtcccccc
     6001 aactctcgcc agatcggcgc atggcggcaa agtctagccg gccggcggac acgatgagca
     6061 cgcccgcgac ggtcaccagg atcgcgaccc acccgcttgc cgagatgcgc tcggagaaca
     6121 gcagcgagcc gatcagcgcg gtcatgatca cgtcggcctt cacgagcatc gtgccgacgg
     6181 caaaattccg catctggaac gcgtagacga ggagcgccgt cccgaagtac tggccgagcc
     6241 cggagaggag gcacatgagc aggaaccgcc cattgaactc cgggattcgc agggagttcg
     6301 tggcgccggc cgcctcgtag gcgacgacgg cggcgagata ggcgagcatc acgggcaggc
     6361 cgaagagtcc ccgcacgagc gtcgcggcaa acaccgaaat gctctggttg gcgaccttta
     6421 agcccgcgag ccgcagcgcc tggaaaaacg ctgccgcgac cgcgagcggg atccagatct
     6481 ggtccatcac accgggcttg atctgagccg cggccaggcc tgcagccgtc gcgccgcctc
     6541 ggccatggcg gcggtgctgc cggaatagga gaagcgcagg aagcgctggc cgcggccggg
     6601 atcgaagtcg atgccgggcg tggcggcgac gccagcttcg gtaagcatcg ccttcgcaaa
     6661 ggcaaggctg tcgttggtga agcggccgac gtccgcatag agatagaagg ccccatcagc
     6721 cggcacgatc tcactgaacc cggccttcgg cagcaagtcg agcagcagct cccgatttgc
     6781 ggcatagacg cgcttgttcg cctcgagctc atcgatgccg tcgaaggcgc cgagggcggc
     6841 cacctgcgag attgccggcg gcgagatgta gaggttctgc gcgagacgct cgagcggacg
     6901 cacgagcctc tccggcacca ccatccagcc gacgcgccag cccgtcatcg agaagtactt
     6961 cgagaagctg ttgatgacga tcacgtcgtc ggaggttgca agcgccgtct gctgccgcac
     7021 gccgtaggtg aggccgtggt agatctcgtc cgagatgaac cacatgccct gctggcggca
     7081 ggtgcggatg atctcggcga ggcggtcggg ggcgagcatg gtgcctgtcg ggttagccgg
     7141 actcgcgagc agcaacccct tggcgcgact gcctcgggcc gcctcggcaa tggcctctgg
     7201 cgttggcgcc cagcgcgtcg cagggccggt gtcgatcatc acaggctcgc agccgagtgc
     7261 cgtcaggatg tggcgatagc aggggtatcc tggagccgcc agcgccacgc gatcgccggc
     7321 atcgaagatg gcgaggaacg cgagcacgaa ggccgcggac gagccgcagg tgacgacgat
     7381 gcgctcgggc gcgacagcaa ccccgtacca gtcgcggtag agcccggcga tgcgttggcg
     7441 gagcggctcc atg
//
LOCUS       DAORNH010000229         7395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7410_length_7395_cov_2.724659, whole genome shotgun sequence.
ACCESSION   DAORNH010000229 DAORNH010000000
VERSION     DAORNH010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7410_length_7395_cov_2.724659"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    290..322
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            380..673
                     /locus_tag="PK264_18945"
     CDS             380..673
                     /locus_tag="PK264_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77987.1"
                     /translation="MSATDAKPASAVDVMTRYANHLNVALVQVISDHMPPETAARQVR
                     DASRTVAVGVIAHILGRIIAGADDHDKKGGRTALTKLARETLDDKITKTERRS"
     gene            670..1080
                     /locus_tag="PK264_18950"
     CDS             670..1080
                     /locus_tag="PK264_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77988.1"
                     /translation="MTERTLPENMNQIELAAYLGLEAAMALAHGTATAAGWYLDPRTK
                     QPIARNFGEVIALMHSELSEALEAHRKKLMDDKLPCRPGIEVELADCVIRILDTAKSL
                     NLDLAGALIEKNRFNAARSDHRLESRGKPGGKAY"
     gene            1454..1741
                     /locus_tag="PK264_18955"
     CDS             1454..1741
                     /locus_tag="PK264_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014529258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD77989.1"
                     /translation="MAHGLTRNGPPRIEPRGLSCAEAAAYLGIRATLFKQLVFEGRLP
                     AAVKLAGRRVWDRHALDKAFDRLGQEPAARPACDASQVPSPTGDIWDNPRV"
     gene            1810..2940
                     /locus_tag="PK264_18960"
     CDS             1810..2940
                     /locus_tag="PK264_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRD77990.1"
                     /translation="MHIKLRYVHEDVDRHGNVRIYFKRPGERKVRLRGEPGSEGFMAA
                     YHAAVAHAEAGDLARPSEPNEHPLAARTGTLRWLADQWRGSAAFAELDPSTQRARRYL
                     IEAMLQEPVQPGSAETFADYPLERLTRRALEVLRDRKAAAPSAANGRVKALRAILKWG
                     LKQEHLTSNPAADLERLREARGGYHTWTEDEIAAFEARHPLGSKARLAMTLMLYAGAR
                     RSDVVRLGRQHVRGSTIKWRAFKNRNRHPVTIELPVADELAAAIAATPTGDLTFLVTE
                     YGRPFSHAGFGAWMKKRCLEAGVSADCSSHGLRKASATRLAEAGATAHQLMAIFGWSS
                     LKEAEVYTRAAERRRMAADGMARVPTRTASSVPPNIPTKATR"
     gene            complement(2965..3050)
                     /locus_tag="PK264_18965"
     tRNA            complement(2965..3050)
                     /locus_tag="PK264_18965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3015..3017),aa:Leu,seq:caa)
     gene            3435..3815
                     /locus_tag="PK264_18970"
     CDS             3435..3815
                     /locus_tag="PK264_18970"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HRD77991.1"
                     /translation="MADVNVHFHGIDRSEAVVQKAQGYAEKLTRTFDRITHCRVAIEA
                     PHRHAHKARVYLVRIDVGLPGRSPVIISSDSDYNPAHEDVLIALKDAFEAARRQVAAV
                     ADQMRSPAKRERGRRRPAPERDLA"
     assembly_gap    3982..4098
                     /estimated_length=117
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4099..4752
                     /gene="paaC"
                     /locus_tag="PK264_18975"
     CDS             <4099..4752
                     /gene="paaC"
                     /locus_tag="PK264_18975"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HRD77992.1"
                     /translation="EEDIALSNMGLDLIGQARLFYTLAGEVEGQGRDEDRLAYFRDAP
                     EFRNVLLVELPNGDFARTMARHLVYASFAHPFMSALAGSNEARLAEIAAKAEKELAYH
                     VRHASEWVIRLGDGTDESRGRMIAGLEFVWGYGAELETMDALERELALAGVAVDRAPI
                     SARWTATIAGVLAEAGLEWPKDRFPQSGGRSGRHTEHLGHLLAVMQSTARADPEAIW"
     gene            4758..5279
                     /gene="paaJ"
                     /locus_tag="PK264_18980"
     CDS             4758..5279
                     /gene="paaJ"
                     /locus_tag="PK264_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HRD77993.1"
                     /translation="MATRQASDPADEVDGDLAKAYAAAAAVPDPEIPVLTLGDLGVFR
                     GVERRDGRIVVRLTPTYTGCPATLAIRLAVEAALLDAGLGDARVETVLSPAWTTDDIT
                     AEGRRRLEAYGIAPPARRAGSIKALFEADTVRCPHCRSAETERIAEFGSTACKALYRC
                     GACREPFDYFKCI"
     gene            5302..6384
                     /gene="paaK"
                     /locus_tag="PK264_18985"
     CDS             5302..6384
                     /gene="paaK"
                     /locus_tag="PK264_18985"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HRD77994.1"
                     /translation="MASGFHTLTVGEVRRETADAVSLRLDVPAALAATFAFRPGQYLT
                     LRAMLDGEDVRRSYSICSALDDGEIRVGIKAVPGGAFSTFANTRLAAGDRIEVMEPDG
                     RFTPTLEPGRAKRYLLIAAGSGITPILSIAATALSREPRSEIFLVFGNRSSRSMMFAE
                     AIEDLKNRHLGRMSVLNVFSREQQDVALANGRIDGGKIRAIAPALVDPGTLDDVFICG
                     PEAMIGEVREALRGLGVSAERIHVELFETAATTGRRQRPDATRATAGEAIATVEAIVD
                     GQRHTFPFSAGDGGVVDAAERAGIELPYSCRGGMCCTCRAKVVSGTSVMATNYSLEPW
                     ELAAGFTLACQTRPTSERLLLDFDQT"
     gene            6434..7027
                     /locus_tag="PK264_18990"
     CDS             6434..7027
                     /locus_tag="PK264_18990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRD77995.1"
                     /translation="MPTQANPSLTTVDWSRIPAPNDDGAARHLAGMAIADIALHATDG
                     SNVSLARLSGRTVVFAYPRTGQPGKLGLVDDWDQIPGARGCTPQSCAFRDQNRDILAA
                     GADRLYGLSTQDTAYQREAVDRLHLPFPLLSDADLAFTHAMRLPTMSVAGQTLLKRMS
                     WIVDDGRITAVQYPVFPPDRNAADVLAWLAANPRTRS"
     gene            7034..>7395
                     /locus_tag="PK264_18995"
     CDS             7034..>7395
                     /locus_tag="PK264_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD77996.1"
                     /translation="MTGAIARSGLVGANVVCLGLGYSASVIAERLVGLGAAVVGTRRR
                     TGKAEVSDHDAAVRVLAFDGSPPGVALAERIAAATHLIVSIPPGEGGDPALAALGPAI
                     AAAPRLGWIGYLSTIGVYG"
BASE COUNT         1225 a         2488 c         2378 g         1154 t
ORIGIN      
        1 tgcgatcctg gtcgccatcg cggtcgggtt cgcggccgtc gtcgcaacgg cgccccgccg
       61 ccccgagccc cgaggtcagc cgcggccggg cccggacatc caggtcgcag ttcttgcagt
      121 cacattcgag gagcccacgc catgacgatc aacgagttga gcaagcggac tgacggcatc
      181 aacgaggcta tggcagaggc cgcgaagtcg cttgccagac ccgcctacac caacggcttc
      241 ggcatcgtca gcgaccccag cgccgcgttg tccaatgtca tcgacgcccn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnatggccaa cggatgccga ctacgacgcg atggagcgcc
      361 agcacaatcg gggggaggta tgagcgcaac cgacgccaag cccgcaagcg ccgtcgacgt
      421 catgacgcgc tacgccaacc atctcaacgt cgccctggtc caggtgataa gcgatcacat
      481 gcccccggag accgcggcga ggcaggttcg cgacgcgtcg cgaactgtcg cggtcggcgt
      541 gattgcgcac atcctggggc ggatcatcgc cggggctgac gatcacgaca agaagggtgg
      601 ccggaccgcg ctgaccaagc tggcccgaga gacgctcgac gacaagatca cgaagacgga
      661 gcggcgctca tgactgagcg aaccctgcct gaaaacatga accagatcga gcttgccgcc
      721 tacctcggcc tcgaggctgc gatggcactt gcgcatggca ccgcgacagc tgcgggctgg
      781 tatctcgacc ccaggacgaa gcagccgatt gctcgcaact tcggcgaggt catcgccctc
      841 atgcactctg aactgtcgga agcgctcgaa gctcatcgca aaaagttgat ggacgacaag
      901 ctgccttgtc gaccagggat cgaggtcgag ctcgctgatt gtgtcattcg aattctcgat
      961 acggcgaagt ctctcaatct cgatctggcc ggtgccctga tcgagaagaa ccgcttcaat
     1021 gcggctcgct ccgatcacag gctcgaaagc cgcggtaagc cgggcggcaa ggcctactga
     1081 tgatcagtca catggccccc cgcagcgctc aaaccgccac acctcggccc aagtgtcgaa
     1141 acggtcgtct tccatggtcg cggcggcact gtcgtcaccc tgcgccaagg agttgcctta
     1201 tgaccgatcg tgagccgacc aatccacctg cccgcgtcga gatcgtcgtc tgctggtcat
     1261 gcggggggca cggagtcccg acctcggagt gcatatgcct taaggaccac tgccagtgcg
     1321 tgagccgcgg cgtggcgcct tgtgtcgatt gcgtgggcag cggccgcatc gccctgcctg
     1381 tcccggcacc gcgtcccggg ttcacctacg agcccgatcc cctccacccc agtgacgagc
     1441 cggaggtctg gccatggcgc acgggctgac ccgcaacggt cctccccgga tcgagccgcg
     1501 cggcctctcc tgcgccgagg ccgccgccta tctcggcatt cgcgcgacgt tgttcaagca
     1561 acttgtattc gaaggtcgcc tgccagctgc cgtgaaactc gccggccgcc gcgtctggga
     1621 ccggcacgcg ctcgacaagg ctttcgaccg ccttgggcaa gagcccgctg cgcgcccagc
     1681 ctgtgacgcg tctcaggtgc cgtcaccgac cggcgacatc tgggacaacc cgcgagtatg
     1741 acgatgctgg acacctcacg tcgggacgcc gatcaatgcc ctcgatcagg gcgagaggcg
     1801 agccgggcca tgcacatcaa gctgcgctac gtgcacgagg acgtcgaccg gcacggcaac
     1861 gtgcgcatct atttcaagcg accgggcgag cgcaaggtgc ggctccgggg cgagcccggc
     1921 agcgaaggct tcatggcggc ctaccacgcg gccgtcgcac atgccgaggc cggcgatctg
     1981 gcgcggccga gcgagcccaa cgaacatccg ctcgcggctc ggacaggcac gttgcgctgg
     2041 ctcgctgacc aatggcgcgg atcagccgcg ttcgccgagc tcgacccctc gacccagcgc
     2101 gcgcgccgct acttgattga ggccatgctg caagagcccg tccagccggg ctccgccgag
     2161 acattcgccg actacccgct cgagcgcctc acacggcgcg ccctcgaggt gctgcgcgat
     2221 cgcaaggccg cggcgccgag cgccgccaat gggcgcgtga aggcgctgcg cgccatcctg
     2281 aagtgggggc tgaagcagga gcatctcacg tcaaatccgg ccgccgatct cgagcggctg
     2341 cgcgaggccc gaggcggcta tcacacctgg accgaggacg agatcgcggc cttcgaggcg
     2401 cggcatccgc tcggcagtaa ggcccgtctc gctatgacgc tcatgctcta cgccggcgcc
     2461 agaagatcgg acgtcgtgcg cctcggccgg cagcacgtgc gcggcagtac gatcaaatgg
     2521 cgggccttca agaaccgcaa tcgccatccc gtcacgatcg agctgccggt ggccgacgaa
     2581 ctcgccgccg cgatcgccgc cacgccgacg ggcgatctca ccttcctggt gaccgagtac
     2641 ggccgcccct tcagtcacgc cggattcggg gcctggatga agaagcgctg cctcgaggcc
     2701 ggcgtgtcgg ccgactgctc ctcgcacggc ttgaggaagg cgagcgccac gcgcctcgca
     2761 gaggccggtg ccacggcgca tcagctcatg gccattttcg gctggtcgtc gctcaaggag
     2821 gcggaagtat acacccgggc cgccgagcgc cgccggatgg ccgccgacgg catggcccgc
     2881 gttcccaccc gcaccgcatc gagcgtccca ccaaacattc ccaccaaggc aacccgttga
     2941 aatggttaat tcttcaataa atgttggtgc cccaggaggg acttgaaccc ccacgtcctt
     3001 gcggacaccg gattttgagt ccggcgcgtc taccgttcca ccactggggc cgccgcgagg
     3061 cgggcgttgg gcgtctagca tcctcgccgc ggcacttcaa cgccagaagc gcgaggccgc
     3121 aaacgcgagg agcgggacgc ttcgcaagga gatgccgtgt tttctcggag ggggtggggt
     3181 gcggcaaccc tggataccgc accccgtgga tcacgccggg gaagtttgca ccagggtgta
     3241 ccgacgctag atccgggcgc ttgaacccac cttgaatatc gcattaatcg gccgttcatc
     3301 tcgccggggc aaccggcaag ggtgcgtcaa ccgtgtggcg tgcacttgcg agatgacgtc
     3361 gccttgaagc ttgtcggctc tttgcctacg tgattcactt ggaggccctg aagctcgaac
     3421 caaggagccg acacatggca gacgtcaatg tccatttcca cgggatcgat cgaagcgagg
     3481 ccgtggtgca gaaggcccaa ggctacgccg agaagctgac gcggacattc gatcgtatca
     3541 ctcattgccg tgtcgccatc gaggcgccgc atcgccatgc ccacaaggcg cgcgtctacc
     3601 tcgtgcgcat cgatgtcgga ctgcccggcc ggtcgccggt gatcatctcg agcgacagcg
     3661 actacaaccc cgcgcacgag gacgtgctga ttgcgctaaa agacgccttc gaggcggcgc
     3721 ggcgtcaggt ggcggccgtc gccgaccaga tgcgctcacc tgccaaacgc gagcgcggcc
     3781 gccgacgccc ggcgcccgag cgcgatctcg cgtgagatcc cccgggtgac cgcgccatag
     3841 cccatcggcg cccttggcga tcggcgagcg aggttgctaa tcttcgccaa ggccgggccc
     3901 gagcaccggc cggcgaaccg cggaaccgga tgcgatgcct gatagagcaa tgccagcacc
     3961 agggccatcc ggacacctgc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnga ggaagacatc gccctctcga acatggggct cgatctcatc
     4141 ggtcaggcgc ggctcttcta cacgctcgcc ggagaggtgg agggacaggg ccgggacgag
     4201 gatcgcctcg cctactttcg cgatgcgccc gagttcagga acgtcctgct cgtcgagctt
     4261 ccgaacggcg actttgcgcg gacgatggcg cgccacctcg tctatgcgtc gttcgcgcac
     4321 ccgttcatga gtgctctggc cggctctaat gaggcccgtc tcgctgagat tgcagccaaa
     4381 gccgagaagg agctcgccta tcacgttcgc catgcgagcg agtgggtgat ccggctcggc
     4441 gacggcacgg atgaaagccg gggccgcatg atcgcagggc tcgagttcgt gtggggctac
     4501 ggcgccgagc tcgaaacgat ggacgctttg gaacgcgaac tcgccttggc cggggtcgca
     4561 gtggaccggg cgccgatctc ggcacgctgg acggcaacca ttgcgggcgt tctggcggag
     4621 gcaggactcg agtggccgaa ggaccgcttt ccgcaatcgg gcgggcgaag cggccggcat
     4681 acggagcatc tcgggcacct gctcgccgtg atgcagtcga cggcacgtgc cgatccggaa
     4741 gcgatatggt gacgaccgtg gcgaccaggc aagcgagcga tccggcggac gaggtcgatg
     4801 gcgatctcgc gaaagcctac gcggcagcgg ctgctgttcc tgatcccgag atcccggtgt
     4861 tgacgctcgg tgacctcggc gtgttccgcg gcgtcgagcg gcgcgatggc cggatcgtcg
     4921 tgcgcctcac gccgacctac accggctgcc ccgcaaccct cgcgatccga ctcgctgtcg
     4981 aggcagcgct gctcgatgcg gggctcggcg atgcccgggt cgagacggtg ctgtcaccgg
     5041 cctggaccac ggacgacatc accgccgagg gccgccgccg gctcgaagcc tatggcatcg
     5101 cgccgccggc acgtcgcgcg gggtcgatca aggcgctctt cgaggccgac acggtacgct
     5161 gcccgcactg ccgctcggcg gagaccgagc gcattgccga gttcggctcg acggcctgca
     5221 aggcactcta ccgctgcggc gcttgccgcg agcctttcga ctacttcaag tgcatttgac
     5281 gcgttgagca gggcgagcgc tatggcgagc ggtttccata ccttgactgt cggcgaggtc
     5341 cggcgcgaga cggcagatgc cgtgtcgctg cggctcgatg tgccggcggc gcttgccgcg
     5401 acgttcgcct tccggcccgg ccagtacctg accctcaggg ccatgctgga cggggaggac
     5461 gtgcgccgca gctactcgat ctgctccgcc ctcgacgatg gggaaatccg cgtcggcatc
     5521 aaggccgtgc cgggcggcgc gttctccacg tttgccaaca cgcgcctcgc cgccggcgac
     5581 cgcatcgagg tgatggaacc ggatgggcgc ttcacgccga cgctcgagcc tggtcgcgcc
     5641 aaacgctacc tgctgatcgc cgcgggctcg ggcatcacgc ctattctgtc gattgcagca
     5701 accgcgcttt cgcgcgagcc gcgctccgag attttcctcg tgtttggcaa ccgcagctcg
     5761 cgctcgatga tgtttgccga ggcgatcgag gatctgaaga accgccatct cggccgcatg
     5821 tccgtgctca atgtcttctc gcgcgagcag caggacgtgg cgctcgccaa cggccggatc
     5881 gacggcggca aaatcagggc aatcgcgccg gcgctcgtcg atcccggcac gctcgacgac
     5941 gtcttcatct gcggtcccga ggcgatgatc ggcgaggtcc gcgaggcgct ccgtggcttg
     6001 ggcgtctcgg cggagcgcat tcatgtggaa ctcttcgaga ctgcggcaac taccgggcgg
     6061 cggcaacgcc ctgatgccac gcgagcgacg gccggcgaag ccatcgcaac ggtcgaggct
     6121 atcgtcgacg gccagcgtca cacgtttccg ttctcggccg gcgatggtgg cgtggtcgac
     6181 gcggccgagc gggccggcat cgagctgccc tactcctgcc gcggcggcat gtgctgcacg
     6241 tgccgcgcca aggtcgtctc cggcacgtcg gtgatggcga ccaactactc actggagccg
     6301 tgggaactcg ccgccggctt cacgctcgcc tgtcagacca ggccgacgag cgagcggctg
     6361 ctcctggact tcgaccagac ctgacagcac atacaagcgt cgctcgaaca ggacgtgttg
     6421 cacgaggacc gccatgccga cccaagcaaa cccgagcctc acgactgtcg actggtcccg
     6481 cattccggcg cccaatgacg acggcgcagc gcggcacctc gccgggatgg cgatcgccga
     6541 tatcgcacta catgcaaccg acgggtcgaa cgtgtctctc gctcggcttt ccgggcgcac
     6601 cgtggttttc gcctatccgc gaacggggca gccgggcaag ctagggctcg tcgatgattg
     6661 ggaccagata ccgggcgcgc gcggctgcac gccgcaatcg tgcgccttcc gcgaccagaa
     6721 ccgcgatatc ctggccgccg gcgcggatcg cctttacggc ctctcgacgc aggataccgc
     6781 ctatcaacgc gaggccgtcg acaggctgca tctgcccttc ccgctgctct cggacgctga
     6841 ccttgcattc acccacgcca tgcgcctacc gaccatgtcg gtcgccggcc agacgctgct
     6901 caagcggatg tcgtggatcg tcgacgacgg gcggatcacg gcggtgcagt acccggtttt
     6961 tccgcctgac cggaacgcgg ctgatgtgct cgcctggctc gcggccaacc cccgaacgcg
     7021 gagctgatcc tggatgacgg gcgccatcgc gcgatcgggc ctcgtcggcg ccaatgtcgt
     7081 ctgcctgggg ctcggctatt cggcgagcgt catagccgag cggcttgtcg ggctcggcgc
     7141 cgccgtagtt ggcacaagac gccgcaccgg aaaggccgag gtgagcgatc acgatgccgc
     7201 tgttcgcgtc ctcgccttcg acggttcgcc tccgggcgtg gcgcttgcgg agaggatcgc
     7261 ggcggcgacg caccttatcg tctcgattcc gcccggcgaa ggtggcgacc cggcgctcgc
     7321 ggccctcggc ccggccatcg ctgccgcgcc acgcctcggc tggatcggtt acctctcgac
     7381 gatcggcgtc tatgg
//
LOCUS       DAORNH010000230         7358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7458_length_7358_cov_3.021909, whole genome shotgun sequence.
ACCESSION   DAORNH010000230 DAORNH010000000
VERSION     DAORNH010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_7358_cov_3.021909"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK264_19000"
     CDS             complement(<1..353)
                     /locus_tag="PK264_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRD77997.1"
                     /translation="MSDLLSRIATDRSDEAFRSLFDEYGPRVRSFMLRLGVEPHVADE
                     LAQETLIVVWRKAGLYSAEKGAVSTWIYTIARNLRTDYARRRRIWQELTDEHTATLSS
                     DETPADDLVGEGVHGL"
     gene            808..1290
                     /locus_tag="PK264_19005"
     CDS             808..1290
                     /locus_tag="PK264_19005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD77998.1"
                     /translation="MTLTPKPCWPPACHVGSLVMSSQLQRAIDPNPSHAHAYYGLGHA
                     FYVAGRPLDALSPLATSLRLSPKEPLARMFLSVAAFCHLMFDDLPAAEAAARRARNLH
                     SEEPWSRLALAATPQIRGDAADARSAIADAREIEPGLTLSTFALLVQPVPRGKAGSRA
                     "
     gene            complement(1365..2771)
                     /locus_tag="PK264_19010"
     CDS             complement(1365..2771)
                     /locus_tag="PK264_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD77999.1"
                     /translation="MPPAKTVAIIGAGPVGLAAAAHALERGLVPVVLEAGPEAGHSVR
                     QWSHVRMFSPWEYNIDKAAERLLVGAGWNAPDPKAYPTGGDMIARYIEPLATRTPLRE
                     RIKTSALVTAISRVGFDKAKTKGREAAPFQIRYQNGKGPETLLADAVIDASGTWLAPN
                     PAGANGLAAVGERDLEKRIAYGMPDILGAERARYAGKTIAVLGAGHSAIGTLIDLVQL
                     ASEAADTRVVWLLRGDRPEKAFGGGANDKLAARGELGSTFATLVRDGKIQVETSFGVT
                     HLTERQGRLRIAAGSACCGRHVIADELIVATGFRPDLSMLSELRLGLDPALDCPAKLA
                     PLIDPNEHSCGTVRPHGARELSHPEPGFYIAGMKSYGRAPTFLTLTGYEQVRSIVADI
                     AGDEEAAARVELVLPETGVCSRAVVESDAAGCCGGPAKTDASACCAVDEAAKAGGKAG
                     CGSASPAQSKGKAQACCG"
     gene            complement(2820..3143)
                     /locus_tag="PK264_19015"
     CDS             complement(2820..3143)
                     /locus_tag="PK264_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD78000.1"
                     /translation="MKIDDAAARLEALGNPTRLRIYRALVKAGDAGLSVGKLQTRLDV
                     AASTLSHHLKTMLIVGLISQERQSTTLICRANYDVMRALLAFLAEECCAEAACSPMPT
                     RSTAA"
     gene            complement(3201..3872)
                     /locus_tag="PK264_19020"
     CDS             complement(3201..3872)
                     /locus_tag="PK264_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRD78001.1"
                     /translation="MTFDLNRRLAAEALGTALLVATVVGSGIMAAKLAGGNMAVALLG
                     NTIPTGAILFVLITILGPVSGAHFNPAVSLVMSLRGELPMREAGLYTVAQIVGGCLGT
                     LAAHAMFALPLLQLSTTARTGGAQWFAEFVAAFGLLATIVGTLRFRADAVPTAVGLYI
                     TAAYWFTASTSFANPAVTLARSISDTFAGISPSHVVAFVVAQLVGALAGAGIMSWLLN
                     APGKR"
     gene            complement(3915..4811)
                     /locus_tag="PK264_19025"
     CDS             complement(3915..4811)
                     /locus_tag="PK264_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD78002.1"
                     /translation="MHVDSNQSSAAVAAVYSALAQETRLEAYRLLLRYQPFGLSAGDI
                     ARLLAVPHNTLSTHMGLLVAVGLVRARKDGRAMIYAAVPERLTRAQSYLDEARVVRPR
                     RLQTAKGASIFPVRRTEDDVSGKIYNVLVLCTGNSARSILAEAVINREGQGRFRAYSA
                     GSHPKGEPNPVGIELLRSLGYDVSAFRSKSWSEFAEPGAPKMDFIITVCDSAAGETCP
                     YWPGHPLVAHWGIPDPAEASGSPAARRAAFQEAYRRLTTRITAFVNLPIDSLSLPELK
                     ARLAEIGAMEGATEMARAGGTA"
     gene            5007..6635
                     /locus_tag="PK264_19030"
     CDS             5007..6635
                     /locus_tag="PK264_19030"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRD78003.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGALLQARGYTVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVPAKKSDNITTGRIYQEILAKE
                     RRGDYLGGTVQVIPHVTDAIKQFVLTGNEGVDFVLVEIGGTVGDIEGLPFFEAIRQLG
                     NDLPRGHSVYIHLTLLPWIPSAGELKTKPTQHSVKELRSIGIQPDILLCRSDREIPAG
                     ERRKIALFCNVRESAVIQALDVASIYDVPLAYHREGIDREVLAAFGIEGAPAPRLERW
                     ERISRTIATPEGEVTIAIVGKYTGLKDAYKSLIEALVHGGIANNVRVKLEWIESEVFE
                     KEDPAPYLEGVHGILVPGGFGERGSEGKILAARFARTRNVPYFGICFGMQMAVIEAAR
                     ALAGVPNAGSTEFGATPEPVVGLMTEWMRGNELEQRKAGGDLGGTMRLGAFDAELAKG
                     SRIAQLYGTTRISERHRHRYEVNTGYRARLEAAGMVFCGLSPDGLLPETVEIPDHPWF
                     IGVQYHPELKSRPFEPHPLFKGFIAAAIDQSRLV"
     gene            6710..7201
                     /locus_tag="PK264_19035"
     CDS             6710..7201
                     /locus_tag="PK264_19035"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HRD78004.1"
                     /translation="MFLHQGGCLCGEIRYETTGEPARIVFCHCRFCQRATGTSFLLEA
                     MFARSDFRILKGEPRRYELTSVGSGKQVVVNLCANCGTKLFLDLERVPNDIGLYTGTY
                     DDPNWFERLGRNAKHIFLDSAQRDTLIHASVPVFREHVTSKEGTATMPEIFATIHQVK
                     CRA"
     gene            7189..>7358
                     /locus_tag="PK264_19040"
     CDS             7189..>7358
                     /locus_tag="PK264_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRD78005.1"
                     /translation="MPSLTTACDTSTATAAPQIYVDADACPVKDEIVRVAERHGLAAV
                     FVSNAWMRLPDS"
BASE COUNT         1244 a         2397 c         2455 g         1262 t
ORIGIN      
        1 aggccgtgga cgccctcgcc gacgaggtcg tcggccggcg tttcgtcgga cgacagcgtc
       61 gcagtgtgct cgtcggttag ctcctgccag atgcggcggc gacgcgcgta gtcggtgcgc
      121 aagttgcgcg caatcgtata gatccaggtc gagacggccc ctttttcggc cgaatagaga
      181 ccggctttcc gccaaacgac aatgagcgtc tcctgggcga gttcgtcggc cacatgcggc
      241 tccacgccga ggcgcagcat gaagctgcgg actcgggggc catactcgtc gaacaggctt
      301 cggaacgcct catcgctgcg gtccgtcgca atccgggaca gcagatcgga catggctccc
      361 ctggctggtc gtcgcaggcc cgcatctctc ggccgggctg cggtttcggg gtgcaacata
      421 agacctccgt ggcttcatgc aagactacgg aggtaggccg gtcgcggatc actttctggc
      481 actccgaacg cgaacgagtg cacgtgatcc gagcgggcga ggggacgtaa caaaacttca
      541 gccgtctggc ggaggttgca gcaaccgctg ccgccgcgtg gccccattag ttctcccgtt
      601 tggatgccgg cagccggtgc gacgtcgatg cggcgatggc actctcgaac gaggacagca
      661 tcgttgccgg catcatgggc gccgttgatg cgggcgtgcg ccgtgccgag cgcgaaggcg
      721 cccgcgccag accgatcgtc caactcgatg cttggagcct gttccatcgc ggcttgtggc
      781 acgtgaatcg gcttacccgc gatgagcatg acgctcacgc ccaagccgtg ctggccgcct
      841 gcttgccatg tggggtcact ggtgatgtcg agtcagttgc agcgtgccat cgacccgaac
      901 cccagtcacg cgcacgccta ctatgggctc ggacatgcgt tctacgtggc aggtcggccg
      961 ctggacgccc tttccccgct cgcaacctcg cttcgtctga gcccgaagga gcctctggcg
     1021 agaatgttcc tgtccgtggc ggcgttctgc catctgatgt tcgacgattt gccggctgca
     1081 gaagccgccg cgcgacgcgc gcgcaatctg cattcggagg agccttggtc gcgcctcgcg
     1141 ctggcggcga cgccgcagat cagaggtgac gcggcggacg cccgcagcgc catcgccgat
     1201 gccagggaga tcgagccggg cctcacgttg tccacgtttg cgctgctcgt tcaaccagtc
     1261 ccccggggca aggcgggatc gcgtgcttga gaccctcgcc aaggcgggac atccggccag
     1321 ctgagctgtg gagcccgcgc ggggcgcgca ccctgcacgc cggctcaacc acagcaggct
     1381 tgagccttgc ccttcgactg cgccggagat gcgctgccgc agccggcctt gcctccggcc
     1441 ttcgctgcct cgtcgacggc gcagcaggcc gatgcatcag tcttggccgg tccgccgcag
     1501 cagccggcag catcgctctc gacaaccgct cgcgagcaca cgcccgtttc cggcagcacc
     1561 agttcgaccc gggctgcggc ctcctcgtcg cccgcgatgt cggcgacgat cgagcgaacc
     1621 tgctcgtagc cggtgagcgt caagaaggtc ggcgcgcgcc catacgattt catgccggca
     1681 atgtagaagc cgggctcggg gtgcgacagc tcccgcgcgc catgcggccg gacggtgccg
     1741 cagctgtgct cgttcggatc gataagcggc gccagctttg ccggacagtc gagcgcggga
     1801 tcgagcccga ggcgcagctc ggagagcatg ctgaggtcgg ggcggaaccc ggttgccacg
     1861 atcagttcgt ccgcgatgac atgccggccg cagcatgccg atccggcggc gatgcggagg
     1921 cggccctgcc tttccgtgag gtgcgtcacg ccgaagctgg tctcgacctg gatcttgccg
     1981 tcgcgcacga gcgtggcaaa tgtcgagccg agctcgccgc gtgccgcgag cttgtcgttc
     2041 gctccgccgc cgaacgcctt ctcgggcctg tcgccgcgca acagccagac gaccctcgtg
     2101 tccgcggcct cgctcgcgag ttggacgagg tcgatcagcg tgccgatggc cgagtggcca
     2161 gctccgagca cggcaatcgt cttgccggca tagcgggcac gctccgcccc gaggatatcc
     2221 ggcatgccgt aggcgatgcg cttttcgagg tctcgctcgc cgacggcggc gaggccattg
     2281 gcgccggccg ggttgggcgc gagccaggtg ccggatgcgt cgatcacggc atcggccagc
     2341 agtgtctcgg gacctttgcc gttctggtag cggatctgga acggcgctgc ctcgcggccc
     2401 ttcgtcttgg ccttgtcgaa accgacgcgg ctgatcgcgg tcaccagcgc cgaggtcttg
     2461 atccgttccc ggagcggcgt ccgcgtagcc agcggctcga tgtaccgggc gatcatgtcg
     2521 cctcccgtcg ggtaggcttt cgggtccggc gcattccatc cagcgcccac cagcaggcgc
     2581 tcggcggcct tgtcgatgtt gtactcccac ggcgagaaca tccgcacgtg gctccactgg
     2641 cggacggagt ggccggcctc gggcccggcc tcgagcacga cgggtaccag tccgcgctcg
     2701 agcgcatgcg ccgcggcggc gaggccgaca gggcctgcgc cgatgatggc gacagtcttc
     2761 gctgggggca tggacataag tcgctccttc tagaaggttt gaaattcaag ccaacagttt
     2821 caggcggccg tggagcgcgt cggcatcggc gagcacgcgg cctcggcgca gcattcctcg
     2881 gctaggaagg cgaggagcgc gcgcatcacg tcataattgg cgcggcagat gagggtggtc
     2941 gactgccgct cctgcgagat gagccccacg atcagcatgg tcttcaaatg gtgcgagagg
     3001 gtcgatgcgg cgacatcgag cctggtctgc agcttgccga cgctgagccc tgcatccccg
     3061 gccttcacga gggcacggta gatccggagc cgtgtcgggt tgccgagagc ctccaggcgc
     3121 gctgctgcat catcgatttt catgatgtgt cgataccagg ctcggctccg actgtcaatg
     3181 gtaattctag gaatatcgaa ttacctcttg cctggagcgt tcagcagcca gctcatgatg
     3241 cctgcgccgg ccaacgctcc gaccaactgt gcgacgacga aggcgacgac atgtgacggg
     3301 ctgatgcccg caaaggtgtc ggagatcgag cgtgcgaggg tgactgcggg attggcgaag
     3361 gaggtcgatg cggtgaacca gtaggcggcg gtgatgtaaa gaccgacggc cgtcggcacc
     3421 gcgtctgccc tgaagcggag cgtgccgacg atggtggcga gcaggccgaa ggccgcgaca
     3481 aactcggcga accactgggc gccccccgtt cgggcagttg tcgagagctg tagcagcggc
     3541 agcgcgaaca tggcatgggc cgcaagcgtg ccgaggcagc cgccgacgat ctgtgcgacg
     3601 gtatagagcc ccgcctcgcg catcggcagc tccccgcgca ggctcatcac caggctgacg
     3661 gccggattga aatgcgcgcc ggagacgggt ccgagtatgg tgatcaggac gaacaggatg
     3721 gcgccggtcg gaatggtgtt gccgagcagt gctacggcca tgttcccgcc ggcaagcttt
     3781 gccgccatga tcccggagcc caccacggtc gccaccagca gggccgtgcc gagcgcttcg
     3841 gccgccaggc ggcggttcaa atcgaatgtc atgtttcccc cactcgcttg caacgtggtc
     3901 gagctttccc ccgatcaggc cgtgccgcct gcccttgcca tctccgttgc gccttccatg
     3961 gcgccgatct cggcgagccg cgccttgagc tccggaagcg acagcgagtc gatcggcagg
     4021 ttcacaaagg cggtgatccg ggtggtgagc cgtcggtagg cctcctggaa agcggcgcga
     4081 cgcgcggccg gcgagccgct cgcctccgcc gggtccggaa taccccaatg cgcgacgagc
     4141 gggtgtccgg gccagtacgg acaagtttcg ccagcagccg aatcgcaaac ggtgatgatg
     4201 aaatccatct tcggcgcccc cggctcggcg aactccgacc aggatttcga gcggaaagca
     4261 ctcacatcgt agcccaggct gcgcagcagc tcgatgccga cgggattggg ctcgcccttt
     4321 gggtggctgc cggccgagta ggcgcgaaag cggccttgcc cttcgcgatt gatgacggcc
     4381 tcggcgagga tcgagcgcgc cgaatttccg gtgcagagca ccagcacgtt gtagatcttt
     4441 ccactcacat cgtcctccgt ccgccgcact ggaaatatgc ttgcaccctt tgccgtctgc
     4501 agcctgcgtg ggcgcaccac tcgcgcttca tcgagatagg attgcgcgcg cgtgagccgc
     4561 tccggaaccg cggcgtagat catcgcgcgg ccatccttgc gggcccgcac cagacccacc
     4621 gcgacgagca ggcccatgtg cgtcgacagc gtgttgtgtg ggacggcgag gaggcgcgcg
     4681 atgtctccgg cgctcaagcc gaagggctgg tagcgcagca acaggcgata ggcctcgagc
     4741 cgcgtctcct gggcaagtgc tgagtagacg gcggcgaccg ccgcgctcga ttggttcgaa
     4801 tccacgtgca tggcaactgc ttagacgcat tctttcatta tatcgagact tatcgacata
     4861 aaatcgctgc tcggagagag gggggacatt ggccagccgc gataagcccg cccgcacagg
     4921 tggaaagaca gccttgagca ggcggccgtg gaggggtgcg catttgccac ttcgaatcga
     4981 gcccggcctg tgtattctgc aagcccatgg cgcggtacat cttcatcacc ggcggcgtgg
     5041 tctcctcgct cggcaaaggg ctggcttcag cggcccttgg agcactccta caggcacgcg
     5101 gctacacggt tcggctccgg aagctcgacc cgtatctcaa cgtcgatccg gggacgatga
     5161 gtccctatca gcacggcgag gtgttcgtca ccgacgacgg ggccgagacg gatctcgatc
     5221 tcggccacta cgagcgcttc acgggcgtgc cggccaagaa gtcggacaac atcacgacgg
     5281 ggcgcatcta ccaggaaatc ctcgccaagg agcggcgcgg cgactacctc ggcggcaccg
     5341 tgcaggtgat cccgcatgtg acggatgcca tcaagcaatt cgtgctgacc ggcaacgagg
     5401 gtgtggactt cgtgctggtc gagatcggcg gcacggtcgg tgatatcgag ggcctgccgt
     5461 tcttcgaggc gatccgccag ctcggcaacg acttgccccg tggccactcg gtctacatcc
     5521 acctgacgct gctgccgtgg attccttcgg ccggcgagct caagacgaag ccgacgcagc
     5581 attcggtgaa ggagctgcgc tcgatcggca tccagccgga catcctcctc tgccgctcgg
     5641 atcgcgagat ccccgccggc gagcggcgca agatcgcgct cttctgcaac gtgcgcgaga
     5701 gcgcagtgat ccaggcgctc gatgtcgcct cgatctacga cgtgccgctc gcctaccacc
     5761 gcgaggggat cgatcgcgag gtgctcgccg ccttcggcat cgagggcgcg cccgcgccga
     5821 ggctcgagcg ttgggagcgc atctcgcgca ccatcgcgac gccggagggc gaggtgacga
     5881 tcgccatcgt tggcaagtac acaggcctga aggacgccta caaatcgctg atcgaggcgc
     5941 tcgtgcatgg cggcatcgcc aacaacgtgc gcgtaaagct cgagtggatc gagagcgagg
     6001 tcttcgagaa ggaggacccg gctccgtatc tcgagggcgt gcacggcatc ctcgtcccgg
     6061 gcggcttcgg cgagcgcggc agtgagggca aaatcctcgc ggcccgcttt gcgcggacgc
     6121 gcaacgtgcc ctacttcggt atctgcttcg gcatgcagat ggcggtcatc gaggcagcgc
     6181 gcgcgctcgc gggcgtgccc aatgcgggtt cgaccgaatt cggcgccacg cccgagcccg
     6241 tcgtcggcct gatgacggag tggatgcgcg gcaacgagct cgagcagagg aaggcgggcg
     6301 gcgatctcgg cggcacgatg cggcttggtg ctttcgatgc cgagctcgcc aaggggagcc
     6361 gtatcgcgca gctctacggc acgacacgga tttctgagcg ccaccgtcac cgttacgagg
     6421 tcaacacggg ctaccgcgcg cggctcgaag ccgccggcat ggtgttctgc ggcctctcgc
     6481 cggacggcct cctcccggag accgtcgaga ttccggacca tccctggttc atcggcgtgc
     6541 agtatcaccc cgagctgaag agccgcccct tcgagccgca cccgctgttc aagggcttca
     6601 tcgcggccgc aatcgaccag agccggctgg tgtaaggggg cagcacgccg gagcgcctcg
     6661 gctcggcgtc agcgtgatcc gccgttggag agccaaatag gggaccataa tgtttcttca
     6721 tcaaggtgga tgtttgtgcg gggaaattcg ctacgagacc acaggcgagc ctgctcgcat
     6781 tgtgttctgt cattgccgct tctgccagcg cgcgacggga acgtcgtttc tgctcgaggc
     6841 catgtttgcg cgctcggatt tcaggattct gaaaggcgag ccgcgtcgct acgagttgac
     6901 ctccgtaggc agcgggaaac aggtcgtcgt caacctctgc gccaactgcg ggacgaagct
     6961 gttcctagat ctcgagcgtg tgcccaacga catcggtctc tacactggca cctacgatga
     7021 cccgaattgg ttcgaaaggt tgggccgcaa cgccaagcac atctttctcg actcggcaca
     7081 acgcgacacg ctcatccatg ccagcgtgcc ggtattccgt gagcatgtca cgagcaaaga
     7141 gggtacggcg acgatgcccg agatctttgc aacaatacac caggtgaaat gccgagcttg
     7201 acaaccgcat gcgacacatc gacggccacc gccgcaccac aaatctacgt cgatgccgac
     7261 gcttgtccgg tgaaggacga gatcgtgcgc gtcgccgagc ggcacggcct cgccgccgtg
     7321 ttcgtctcga acgcctggat gcgcctgccc gacagcta
//
LOCUS       DAORNH010000231         7333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7502_length_7333_cov_2.821654, whole genome shotgun sequence.
ACCESSION   DAORNH010000231 DAORNH010000000
VERSION     DAORNH010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7502_length_7333_cov_2.821654"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>294)
                     /locus_tag="PK264_19045"
     CDS             complement(<1..>294)
                     /locus_tag="PK264_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HRD78006.1"
                     /translation="EALSYDAQVLTGGARGLLPGGRTIDLVHVGTGFADRNQAIPVHF
                     SVSGGTAGLRAGQFLPVLASTAEVRRGVAVPREAVLRGSNGQLLVYEHTNAER"
     assembly_gap    295..317
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<318..1558)
                     /locus_tag="PK264_19050"
     CDS             complement(<318..1558)
                     /locus_tag="PK264_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HRD78007.1"
                     /translation="MFGLLLLALVATVSPKAIAHEGHDHGAPPPPVSKTIAPRADTSS
                     NDFEVVVVARGPTIVVHVDTFRSNEPVEGATLEVDTPAGVLKPAPVPKEPGTYSVAAP
                     FAAKPGKYDLAITVTAKETVDILAATLVVPEPANAAEQAVQVNGLVGVVRKLGQGLAD
                     LTGLMWAYVIAAFILGAIVSRVLWPKRATSAAISAATVTALTVFVTAALTSPVKADTG
                     GATDSGTSARDIAQRFADGAVFVPKPTQRILALRTIFTELAEHRRTIELPGRIVPSPN
                     ASGLVQASVGGRLFPPPGGFKALGTRVKAGDILAFVRPPLSLADATAQAQQARELDQQ
                     ISIVTRKVERLRSIEKVIAKSQLEDAELELKGLTTRRANLDRVKSEPEALVAPVDGIL
                     AAANAVAGQMAEPNAVIFHII"
     gene            complement(1664..2977)
                     /locus_tag="PK264_19055"
     CDS             complement(1664..2977)
                     /locus_tag="PK264_19055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD78008.1"
                     /translation="MMRLLDTLVARLILVSLLGITLVHVLSIWSYEKALERELGHADE
                     ARLAERIQTIRRSIAAVSAREREALAHDLSSGPLEAHWNRTRGTAAGGKGAERWRGLV
                     ERLRVSAGELAPDDIVLGTGDDPHNALLSIRLPDSTWLNVSLFAASRSGHGGHGTLLS
                     TSIMAAGVVLMSLLIAGWLTRPLRSMASAVSALSPDGPRSEIAEEGPREVRQLASAFN
                     DMQTRIGELIDRRTQALAAVSHDLRTPLTRLKLRLSDIGDLALQKSVQADLAEMEAMI
                     EATLAYLRGQETQEPPRPIDLAALLATVVDEARDAGHDAVLIAPPPITVTGRHLGLKR
                     ALTNLAANAVRFGTKVEVRAAISGAVVAIEIADNGPGIPVGQLDAVFEPFVRLETSRN
                     RESGGVGLGLTIAKACIEADRGTLTLANRPEGGLVAVIRLPRQPA"
     gene            complement(2974..3747)
                     /locus_tag="PK264_19060"
     CDS             complement(2974..3747)
                     /locus_tag="PK264_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD78009.1"
                     /translation="MPTNSGSQHQRAPALAPILSRDAHILIVDDDAQIRQLAAKLIRE
                     HGHRVSLARDGREMWQALETATIDLVILDVMLPGGNGLDLCRELRARTALPIIMVTAL
                     GSDTDRIVGLEIGADDYLAKPFNPRELIARINAVLRRAQASAGTTSERGRDRLGFDGW
                     TLDTRRRELTNPRGAIVDLSTGEYDLLLTLLEAPQRVLTREQLLDGAKNRIATGNDRA
                     IDIQVSRLRKKLDASEDGQAMIKTIRGTGYMLVPAVTRS"
     gene            complement(3944..5893)
                     /gene="acs"
                     /locus_tag="PK264_19065"
     CDS             complement(3944..5893)
                     /gene="acs"
                     /locus_tag="PK264_19065"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HRD78010.1"
                     /translation="MSEKTYAVPAEWASRAWVDQQTYQAMYKRSVEDPAGFWGEMGKR
                     IDWMKPYTRVKNTSFAHENVSIKWYEDGTLNVSANCIDRHLKKRADQVAIIWEGDDPT
                     QDEKITYRQLHERVCKFANVLKANGVKKGDRVTIYLPMIPESAYAMLACARIGAVHSI
                     VFGGFSPDSLAGRIEDAQSTFVITADEGLRGGKPVPLKKNADDAIKKIPGVTGIKVLV
                     VRRTGNAVPWTAGRDLWLHDELVKVTADCPPVEMSAEDPLFILYTSGSTGKPKGVLHT
                     TGGYIVYAAMTHQYVFDYHDGDVYWCTADVGWVTGHSYIVYGPLANGATTLMFEGIPN
                     YPTPSRFWHVVDKWKVNIFYTAPTAIRALMGAGDDFVKRANRGSLRLLGSVGEPINPE
                     AWEWYHRVVGDGRCPIVDTWWQTETGGILITPLPGATKTKPGSATLPFFGIRPALVDE
                     KGNTIEGAASGNLVILDSWPGQMRTVFGDHKRFVETYFSTYKGTYFTGDGCRRDADGY
                     YWITGRVDDVINVSGHRLGTAEVESALVAHPSVAEAAVVGYPHDLKGQGIYCYVTLKA
                     GMQPGDELKKELVATVRKIIGPTATPDLIQFAPGLPKTRSGKIMRRILRKIAEDDFSN
                     LGDTTTLADPAVVDDLVSNRQNKRA"
     gene            complement(6052..7008)
                     /locus_tag="PK264_19070"
     CDS             complement(6052..7008)
                     /locus_tag="PK264_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HRD78011.1"
                     /translation="MLDLPKSVTVVEVAPRDGLQSFDRAVDTATKIAVTDRLSEAGFG
                     VIEVTGFAHPRVIPNLADAEAVAHGITRRPHTVYRGLVPNARGAERAKSTPLDELVGL
                     ITVSETYLAKNQNMKRAEAVEEAIAAFRIAERAGKSFVMALGMSMWCPYEGVIPVEST
                     LALLKQFRNAGMRSFYLAGSLGLEEPKQVYELFQAARRAFPDIELGYHVHNVSGHAPA
                     NILAAIAGGATFVEGAICGIGGGIMMPTTLGSVGNYPTEDLVALLEASGVTTGLDRDA
                     VIDAARDVAALLAITPRSHAANGGTRSAVAEAALRNPRSHPA"
     gene            complement(7051..>7333)
                     /locus_tag="PK264_19075"
     CDS             complement(7051..>7333)
                     /locus_tag="PK264_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD78012.1"
                     /translation="LQLGGGSPDQQRAIYQPFNLVVLALASLAMIVSGQMSARIVPVA
                     LLCLPVTLAAAWIGARLYVGLSPETFKRIVLCLLLASGCILLAETVWRA"
BASE COUNT         1142 a         2407 c         2500 g         1261 t
ORIGIN      
        1 aagcgctcgg cgttggtgtg ctcgtagacg agcagctggc cgttgctgcc gcggagcacc
       61 gcttcgcgcg ggacggcaac gccacggcgc acctcggcgg tcgaggcgag cacgggcagg
      121 aactggccgg ctctgaggcc cgccgtgccg ccgctcaccg agaagtgcac cgggatcgct
      181 tggttgcggt cggcgaaacc cgttccgaca tggacgagat cgatcgtgcg acctccaggc
      241 aggagaccgc gcgcgccgcc ggtcagcact tgcgcgtcgt agctcagcgc ctctnnnnnn
      301 nnnnnnnnnn nnnnnnntcg atgatgtgga agatcaccgc attcggctcc gccatctggc
      361 ccgcgaccgc gttcgcggcg gcgaggatgc cgtcgacggg cgcgacgagt gcctcgggct
      421 ccgatttcac gcggtcgaga tttgcccgtc gcgtcgtcag tcccttgagc tcgagctcgg
      481 cgtcctcgag ctggctcttg gcgatcacct tctcgatgga gcgtagccgc tcgaccttgc
      541 gggtgacgat cgagatctgc tggtcgagct cgcgagcctg ctgcgcctgg gccgtcgcgt
      601 ccgcgagtga caagggcggg cggacgaatg ccagaatgtc acccgccttc acccgcgtgc
      661 cgagcgcctt gaagcctccg ggcggcggga acagtcgacc gccgacggag gcctgcacga
      721 ggccactggc gttcgggctc ggcacgatgc gtcccggcag ctcgatcgtg cggcgatgct
      781 cggcgagctc ggtgaagatc gtgcggagcg ccaggatgcg ctgcgtcggc ttcggcacga
      841 acacggcgcc gtcggcaaag cgctgtgcga tgtcgcgggc gctcgtcccg ctatccgtcg
      901 cgccccccgt atcggccttg accggagacg tgagtgccgc tgtgacgaag acggtgagcg
      961 ccgtgactgt ggccgccgag atcgcggcgc tcgtcgcgcg cttcggccac agcacgcgcg
     1021 agacgatcgc accgaggatg aacgccgcga taacatacgc ccacatgagg cccgtgaggt
     1081 cggccaaccc ttgcccgagc ttgcggacca cgccgaccag accattcacc tggacggcct
     1141 gctccgcggc attcgcgggt tccggaacga cgagcgtggc cgcgaggatg tcgacggtct
     1201 ccttcgcggt caccgtgatc gcgaggtcgt atttcccggg cttggcggcg aagggtgcgg
     1261 caacgctata ggtgcccggc tccttcggca caggcgccgg cttcaacacg ccggcgggcg
     1321 tatcgacctc gagggtcgcc ccttcgacgg gctcgttcga tcggaacgta tcgacatgca
     1381 cgacgatcgt cgggccgcgc gccacgacca ccacctcgaa gtcgttggag gacgtatccg
     1441 cgcgcggcgc gatggtcttg gacacgggcg gcggcggggc gccgtggtcg tgcccttcgt
     1501 gagcgatcgc ctttgggctc actgtcgcaa cgagcgccag taagagaagg ccgaacaagt
     1561 tcgtcgcgtg cctcagccgc atggcgcctc gcgtcaggag tgacccgagg agaccctaac
     1621 gggatgtcca tgtcggcgct ttgcagcatt gtaacaaatt gtttcaggca ggctgccgcg
     1681 gcaaccgaat cacggcgacg aggcctccct cgggccgatt ggcgagtgtg agcgtgccgc
     1741 gatcggcctc gatgcaggcc tttgcgatcg tgagccccag tccgacgccg ccgctctctc
     1801 gattgcgcga ggtctcgagg cgcacgaaag gctcgaacac ggcgtcaagc tggcccactg
     1861 gaatgccggg cccgttgtcg gcgatctcga tggcgacgac ggcgcctgaa atggccgctc
     1921 gaacctcgac cttggttccg aagcgtaccg cgttggcggc cagattggtg agcgcccgtt
     1981 tgaggccgag atggcgtccc gtcacggtga ttggcggtgg tgcgatcagc accgcgtcgt
     2041 ggccggcatc acgcgcctcg tcgaccaccg tcgcgagcag ggcggcgaga tcgatgggcc
     2101 gcggtggctc ctgcgtctcc tgacctcgga ggtaggcgag cgtcgcctcg atcatggcct
     2161 ccatctcggc gaggtcggcc tgcaccgatt tttgcagtgc caggtcgccg atgtcgctga
     2221 ggcgcagctt caggcgcgtc agcggtgtgc gcaaatcgtg ggagaccgcg gcaagcgctt
     2281 gagtgcggcg gtcgatcagc tcaccgattc gtgtctgcat atcgttgaat gcggaggcga
     2341 gctggcgcac ctcgcgcggg ccctcctcgg caatctcgga gcgtggacca tcgggcgaga
     2401 gcgcactcac cgcggaagcc atcgatctga gcggccgcgt caaccagccc gcgatcagca
     2461 gcgacatgag aacgacgccc gccgccatta tcgacgtcga aagcagcgtc ccatgcccgc
     2521 cgtggccgga ccgcgaggcc gcgaacagac tgacattgag ccaggtggaa tcagggaggc
     2581 ggatcgagag gagggcgttg tgcggatcat cgcccgtgcc gagcacgata tcgtccgggg
     2641 cgagttcgcc ggccgagacg cggagacgct cgacgagccc gcgccagcgc tcggcgcctt
     2701 tgccgcctgc cgcagtgccg cgcgtcctgt tccagtgcgc ctcgagcggc ccgctcgaca
     2761 aatcgtgggc caacgcctcg cgctctcttg ccgagacggc cgcgatgctg cgccggatcg
     2821 tctggatgcg ctcggcgaga cgcgcctcat ccgcgtgacc gagctcgcgc tcgagcgcct
     2881 tctcgtagct ccagatgctc agcacatgga cgagcgtgat gccgagcagc gacaccagaa
     2941 tcagccgcgc tacgagcgtg tcgagaaggc gcatcatgag cgcgtcacgg caggtaccag
     3001 catgtagccc gtgccgcgga tggtcttgat catcgcctgc ccgtcctcgc tcgcgtcgag
     3061 cttcttcctg aggcggctca cctggatgtc gatcgcgcga tcgttgccag tcgcgatccg
     3121 gttcttggcg ccgtcgagga gctgctcgcg tgtcagcacg cgctgcgggg cttcgagcag
     3181 ggtgagcagc agatcgtatt cgcccgtcga gaggtcgacg atcgcgccac gcggattggt
     3241 gagctcgcgc cgacgcgtgt cgagtgtcca gccgtcgaac ccgaggcgat ctcggcccct
     3301 ctcggaggtc gtgccggcgc tcgcctgtgc ccgccgcagc acggcattga tgcgggcgat
     3361 cagttcgcgc ggattgaagg gtttggcgag atagtcgtcc gcaccgatct cgagcccgac
     3421 gatgcggtcc gtatccgagc cgagcgccgt caccatgatg atcggcaacg ccgtcctcgc
     3481 acgaagctcc cggcagaggt cgaggccgtt gccgccgggc agcatgacgt cgaggatcac
     3541 gagatcgatc gtcgccgtct cgagcgcctg ccacatctcg cgcccgtcgc gcgcgaggga
     3601 gacgcggtgg ccgtgctcgc ggatgagctt cgctgcgagc tggcggatct gcgcgtcatc
     3661 gtcgacgatc aggatgtgag cgtcccgcga cagtatcggg gcgagtgcgg gcgcgcgctg
     3721 atgctggctc cctgaattgg tcggcatgct cgttcgctga tgttgcctgg ttcgtccgtc
     3781 gctcttcggc acgcggtccg agtgtagcaa atcggtcgaa ccgtgccagg tcatgatgcc
     3841 tcacggtgtc atgatgcctt gcggccatgc tcctcacgca atcgggccgg agcggtacac
     3901 ccgctccagc ccggatcggc agcgccctgc gaggcctcga aggttacgcc cgcttgttct
     3961 ggcggttgct gacgaggtcg tcgacgacgg ccggatcggc gagcgtcgtc gtatcgccga
     4021 ggttcgagaa atcgtcctcg gcgatcttcc ggaggatccg gcgcatgatc ttgccggagc
     4081 gcgtcttcgg cagacccggc gcgaactgga tgaggtccgg cgtcgccgtc gggccgatga
     4141 tcttgcgcac cgtcgcgacg agctccttct tgagctcatc gcccggctgc atgccggcct
     4201 tcaaggtgac gtagcagtag atgccctggc ccttgaggtc gtgcggatag ccgaccacgg
     4261 ccgcctcggc gaccgaggga tgggcgacga gcgcgctctc gacctccgcc gtgccgagcc
     4321 gatggccaga cacgttgatc acgtcgtcga cgcggccggt aatccagtag tagccgtctg
     4381 cgtcgcgcct gcagccgtca ccggtgaagt atgttccctt gtaggtcgag aaataggtct
     4441 cgacgaaccg cttgtgatcg ccgaacaccg tgcgcatctg gccgggccag ctgtcgagga
     4501 tgacgaggtt ccccgacgcg gcaccctcga tcgtgttgcc cttctcatcg accagcgcag
     4561 gccggatgcc gaagaacggc agcgtcgccg agccgggctt tgtcttggtc gcaccgggca
     4621 gcggcgtaat caggatgccg ccggtctccg tctgccacca cgtgtcgacg atcgggcagc
     4681 ggccgtcgcc gacgacgcgg tgataccatt cccaggcctc cggattgatc ggctcgccga
     4741 ccgagccgag gaggcggagc gagccgcggt tcgcccgctt cacgaaatcg tcgccggcgc
     4801 ccatcagcgc gcggatcgcc gtcggcgcgg tgtagaagat gttgaccttc cacttgtcga
     4861 ccacgtgcca gaagcgcgac ggcgtcggat agttgggtat gccctcgaac atcagcgtcg
     4921 tggcgccgtt ggcgagcggg ccatagacga tatagctgtg gcccgtgacc cagccgacgt
     4981 cggccgtgca ccagtagaca tcgccgtcgt ggtagtcgaa aacgtactgg tgcgtcatcg
     5041 ccgcgtagac gatgtagccg cccgtcgtgt gcagcacgcc cttgggcttg ccggtcgagc
     5101 ccgatgtgta gaggatgaac agcgggtcct ccgcgctcat ctcgacgggc ggacagtccg
     5161 cggtcacctt aacgagttca tcgtggagcc agaggtcgcg tccggccgtc cacggcaccg
     5221 cgttgccggt gcggcgcacg acgaggactt tgatgccggt gacgcccggg atcttcttga
     5281 tggcgtcgtc tgcgttcttc ttgagcggca cgggcttgcc gccgcgcaag ccctcgtcgg
     5341 cggtgatgac gaacgtcgac tgcgcatcct cgatgcggcc cgcgaggctg tccggcgaga
     5401 aaccgccgaa gacgatcgag tgcacggcgc cgatgcgcgc gcaggcgagc atggcatagg
     5461 ccgattctgg gatcatcggc aggtagatcg tgacccggtc gcctttcttg acgccgttcg
     5521 ccttcagcac gttggcgaac ttgcagacgc gctcgtggag ctggcgatag gtgatcttct
     5581 cgtcctgtgt cggatcatcg ccttcccaga tgatcgcaac ctggtcggcg cgcttcttga
     5641 ggtggcggtc gatgcagttc gccgaaacgt tcagcgtgcc gtcctcgtac cacttgatcg
     5701 agacgttctc gtgggcaaag gacgtgttct tgacgcgggt gtagggcttc atccagtcga
     5761 tgcgcttgcc catctcgccc cagaagccgg ccggatcctc gacggagcgc ttatacatcg
     5821 cctggtaggt ctgctgatcg acccaggcgc ggctcgccca ctcggctggg acggcgtagg
     5881 ttttctcgga catgcctcga cctcttggct cgcgtttccc gttcgggccg cttccggccc
     5941 cgatttcctg catgcggcga tcttagaggc cgtgatcagg ctcccgcaac cgccccattc
     6001 gtcggagatc gcgccgcaca gggccgcttc cgaccatgac cgtgcaggct gtcaggcggg
     6061 gtgcgagcgc ggattgcgca gtgccgcttc cgccaccgcg ctgcgcgtgc cgccgttcgc
     6121 ggcatggctc ctcggtgtga tcgccagcag cgcggcgaca tcccgggccg cgtcgatcac
     6181 ggcgtcgcga tcgaggcccg tcgtgacgcc ggaagcttcg aggagggcca ccagatcctc
     6241 cgtcggatag ttgccgaccg agccgagcgt ggtcggcatc atgatgccgc ctccgatacc
     6301 gcagatcgca ccctcgacga aagtcgcgcc gccagcgatt gccgccagga tgttcgccgg
     6361 ggcatgtccg gagacgttgt gcacgtggta gccgagctcg atatccggga aggctcgccg
     6421 ggctgcctga aacagctcat agacctgctt cggctcctcg aggccgaggc tgccggccag
     6481 atagaagctt cgcatgccgg cattgcggaa ctgcttcaga agcgcgagcg tgctctcgac
     6541 cgggattacg ccctcgtagg gacaccacat gctcatgccg agcgccatca cgaacgactt
     6601 gcccgcgcgt tcagcaatcc ggaacgcggc gatcgcctcc tccacggctt cagcccgctt
     6661 catgttctgg ttcttggcga gataggtctc gctcaccgta atgaggccga ccagctcatc
     6721 gagaggcgtc gacttggcgc gctcggcgcc gcgggcgttc gggaccaggc cacgatatac
     6781 cgtatgcggg cgccgcgtga tgccgtgcgc gacggcctcg gcatcggcaa ggttcgggat
     6841 gacgcgcggg tgcgcaaagc cggtcacctc gatgaccccg aacccagcct cgctcagcct
     6901 gtccgtgacg gcgatcttgg tcgccgtatc gacagcgcgg tcgaagcttt gcagcccgtc
     6961 cctaggggcc acctcgacga cggtgacgga cttcggcaga tcgagcatgc agagtcctcg
     7021 gggcgaatag tcatgtgctg ccgacgcgcg tcatgcgcgc cagacggtct cggcgagcag
     7081 tatgcacccg gacgcgagca gcaggcaaag cacgatgcgc ttgaacgtct cgggcgagag
     7141 gccgacgtag agccgggcgc cgatccaggc ggcggcgagc gtcaccggca ggcacagcag
     7201 cgcgaccggc acgatgcgcg cactcatctg gccggagaca atcatggcga gcgaggcgag
     7261 cgccaggacg acgagattga acggctgata gatcgcgcgc tgctggtcgg gactgccgcc
     7321 gcccagctgc agc
//
LOCUS       DAORNH010000232         7319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7516_length_7319_cov_2.657764, whole genome shotgun sequence.
ACCESSION   DAORNH010000232 DAORNH010000000
VERSION     DAORNH010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7516_length_7319_cov_2.657764"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..292
                     /locus_tag="PK264_19080"
     CDS             164..292
                     /locus_tag="PK264_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78013.1"
                     /translation="MKTTIVIAALAAAVALGACRREVAHHPMKLGAEVPAAAAVAR"
     gene            1035..1553
                     /gene="pal"
                     /locus_tag="PK264_19085"
     CDS             1035..1553
                     /gene="pal"
                     /locus_tag="PK264_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HRD78014.1"
                     /translation="MRRARFVAVALLACAVAACANTAPKPDGRFGPGGPGGVGAYGPG
                     GPAAPGSARDFSANVGDIVYFTTDSTDLSPEAQQTLTGQARWLQQYAQYTITIEGHAD
                     ERGTREYNIALGAKRAQIVRDFLVRAGVNPARIRTISYGKERPVAVCNDISCWSQNRR
                     AQTVLNARGVGS"
     gene            1847..>2898
                     /gene="hisC"
                     /locus_tag="PK264_19090"
     CDS             1847..>2898
                     /gene="hisC"
                     /locus_tag="PK264_19090"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRD78015.1"
                     /translation="MTKSPSVPVPKPGILDIHAYVPGESSVPGGLKPIKLSSNETPLG
                     ASPKAIAAYKAEADSLERYPDGGATALRNAIAAKYGLDPTRIVCGCGSDELLNLLAHA
                     YLGNGDEAVFTEHGFLVYKIVTLGNGAKPVVVKCREHRTDVDAIIAALTPRTKIVFLD
                     NPNNPTGTYIPFSEVRRLHAALRPDTLLVLDSAYAEYVRRNDYEAGLELVATSANTVM
                     TRTFSKIYGLAALRLGWAYCPAHIADVLNRIRGPFNVTAPAIAAGVAAINDSAHVDAS
                     LAHNDKWLPWVTAELEKLGLKVNPSVGNFILIHFPADAAHGADAADKHLKSRGIILRR
                     VAGYGLPQCLRMTIGTA"
     assembly_gap    2899..2908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2992..4953
                     /locus_tag="PK264_19095"
     CDS             2992..4953
                     /locus_tag="PK264_19095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD78016.1"
                     /translation="MVLPTLIPRAKLFSNPARTLPQISADGHLLAWLSPVEGVLNIWV
                     APIDDPGAARQLTRRTGRPVMHYDWLPNGRQIAYVSDDNGDENLYVVLVDVATGSERI
                     VTPRTGVAASVAMASPDRPDAMLVLLNERDKRWHDAVEIDLATGAQRIVFENTLGLSS
                     MSFDWQHRCRLGVRQIGGGRQELLRLDGARPEVLFEIPQPDAFSTGPGGFNRNGDHWY
                     LHSSIGRDRSALFRVDALSNRQTLLAEHDTADISGLIADPLTFDVIAACADPIRREWM
                     ALTDAARADLAILKAALPGLELSVVSQSNVGRWVIGSYAGDQPMVYHLYDRTERTVTK
                     LFGVRPELEAYSLAPTHGQIIKSRDGLDLVCYLTLPASETGERPKAPLPMVLHVHGGP
                     WHRDYYGYNRDNQWLANRGYAVLAVNYRASTGFGKAFINAGDKEHAGKMHDDLIDAVD
                     WAVREGIARRDKVAIMGASYGGYATLVGLTFTPDVFCCGVSVVGISNLVTMLENIPPY
                     WEGFNEQMFGRYADPRTEEGRAWLRSRSPLSRIDRISKPLLLGHGANDVRCKQMESDQ
                     IVDAMTARGLPATYVVYTDEGHGFYRPENNMSFNAITEAFLARHLGGRMEPIGSDLDG
                     SSLIVPTGAESIEGLIDKLPPERRLRQAS"
     gene            4964..5914
                     /locus_tag="PK264_19100"
     CDS             4964..5914
                     /locus_tag="PK264_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HRD78017.1"
                     /translation="MLKDSKDPMFGRVALIGIGLIGSSISHACRRGKLAQTIVGAAKT
                     EATRAAALRLGLVDTAYASSADAVGGADLVIMCVPVGACGAVAAEIGPHLRPGAILTD
                     VGSVKGAIVRDCQPHVPKGVHFIPGHPIAGTEHSGPESGFATLFDNRWCILTPPAGAD
                     AAAVDRLAAFWRALGSNVETMDADHHDLVLGITSHVPHLIAFNIVNTAAHLERVTDSE
                     VIKFSAGGFRDFTRIAASDPTMWRDVFLNNREAVLEMLARFTEDLIHLQRCIRFGDGD
                     TMFRLFTEARAIRRGIIQAGQDTAAPDFGRGQVTAPPRRS"
     gene            5927..6769
                     /locus_tag="PK264_19105"
     CDS             5927..6769
                     /locus_tag="PK264_19105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRD78018.1"
                     /translation="MDGIVFLAVLGAALCHAGWNALLKLDLEPLLAITLISVACGVVA
                     IPMLPFAGLLHPSAWPYIAGSLALHLVYYTALAEAYKTGDLGQVYPIARGSAPLMTSI
                     GAAFILDERLGPVGWSGIVLLASGILTLSLGGARSLPANPRTVGFALLTAVSIALYTL
                     VDGAGVRTSQNVAAYVAWLFFLDGLMMAVFGYWYARQRFIEAARAKWRLVAFGGVLST
                     LAYAIALWAMTRAPIGLVAALRETSVLFAAAIGIVFLGERLSRARIIAALLVVAGLML
                     IRLK"
     gene            complement(6762..>7319)
                     /locus_tag="PK264_19110"
     CDS             complement(6762..>7319)
                     /locus_tag="PK264_19110"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HRD78019.1"
                     /translation="LDGATTRITGGIVNGHPFFLWVGAGFDGRVAGALHHGAKRRLGK
                     LAYGGAIARAIAAPLDPLTVEVDGRCQPCGWVVVTNASRYGGRFHLTDRTELSKPGLV
                     AVLLTDQRRGTLAKVLLALATGRLPDLARSERGVACLPATRVVVTAERPISAQVDGDP
                     ERHARLEIRAGGPAFDLIIPPAAST"
BASE COUNT         1203 a         2384 c         2394 g         1328 t
ORIGIN      
        1 caaatgaaga agatcgtcgt tctcgccgct gttgtttcgg ctgttgccct cggcgcctgc
       61 cgcaaggaag tcgagcacaa gccgatgaag ctcggtgcgg aactgccggt tgccgggcag
      121 atcgctcgct aacccgaact gaaactctaa cgggggatct cccatgaaga ccacgatcgt
      181 tatcgccgcc ctcgccgctg ccgtcgccct tggcgcctgc cgccgtgagg ttgctcatca
      241 cccgatgaag ctcggcgcgg aagtgccggc agccgccgcg gttgcgcgct gacgtccctc
      301 cgggggcaac cagtgctgga gacttcggtc tcctcggtgg cgatgaaact cgcatcaagc
      361 taagtcgtca tgacgcaagt gctgcgttag ctcttggtgc cctggtcgag cagtctatcg
      421 tcttcgcata gacacatggc cagacgcaga cgcagtgcgg gagatctacc cgcagactct
      481 cgaggccgcc ctatcgggcg gccttgttgc ttgaatgccg aattcgcccg gcgcaacctc
      541 ccagttcggc ccttgttctg gtgcttgcct gatggccgac gtcgaacgcg cgatctcggc
      601 acggcgtgcg atgcgccgac tcatcggcaa ttgagggcct tccgactgaa gagatcactg
      661 gatcatcggc gatttgccgt ggattgtgag gacgtggacg cccaatccgg ccttgattcg
      721 ggcatactcg tccgaaaggc gtcatagttt cgtgccgggc gtccgctact gaccggtctg
      781 tagagtacgg gaaactgcgt ttctcgacgg ccgccgtgct tcttgtccgg agcaggcctg
      841 gacacaagtc gggtcgcggc gagtgtgcag ggcaatggtg cgcttctggc aatgacgcgt
      901 acccacagtg acaagtgccg gcagtgacga gtgacgattg aactggactt cgatatctgc
      961 cgccgggttt gcggcgaggg ggaatgatga tcaggttctg gagcaacgca catccgatga
     1021 tgggccagcc ggcaatgcgc cgtgctcgct tcgttgcagt cgcccttctc gcctgcgctg
     1081 tcgcggcctg cgcgaatacc gcaccgaagc cagacggccg attcggcccc ggcggcccgg
     1141 gtggcgttgg cgcctatggt ccgggtggcc ccgcggcccc tggctctgcg cgtgacttca
     1201 gcgccaatgt cggtgacatc gtctatttca cgaccgacag cacggacctc tcgcccgagg
     1261 cgcagcagac gctcaccggc caggcgcgct ggttgcagca gtacgcgcag tacacgatca
     1321 ccatcgaggg ccatgcggac gagcggggga cgcgcgaata caacatcgca ctcggcgcca
     1381 agcgcgctca gattgtgcgc gacttcctcg tccgtgccgg tgtgaatccg gcccggatac
     1441 gcacgatctc ctacggcaag gagcgtccgg tcgcggtctg caacgacatc tcctgctggt
     1501 cgcagaaccg acgcgcgcag actgttctca acgcgcgggg agtgggctcc tgaggagcgc
     1561 ggttctggca gtcagaaggg gccggttccg gggcgcccac agccccgggc ggcacggggg
     1621 cagtccggcc gcgcagcaat gctatacccc aaccgcaaca attcgtgctg tgttccgtcc
     1681 aggcacaagc gtcaggtctt cggcggcggt cacggtgcgc tgatgtcctc gactgcctat
     1741 cgattgcgct aggatccacg cgtacactcg actatcacag gcgcgacctt tcgatggcgc
     1801 ggttaggcga cgatcctcag ccgtgccgca catgaggaca ctcgccatga ccaagtcccc
     1861 gagcgtgccc gttccgaagc ccgggattct cgacattcac gcctatgtgc cgggcgagag
     1921 cagcgtgcct ggcgggctga agccgatcaa gctttcgtcc aacgagacgc cactcggcgc
     1981 gtcgcccaag gcgattgccg cctacaaggc ggaggctgat agcctcgagc gctaccccga
     2041 cggtggcgcg acggcgctgc gcaacgcgat tgccgcgaag tacggcctcg atcctacgcg
     2101 catcgtatgc ggttgcggct cggacgagct gctcaatctc ctcgcgcacg cctatctcgg
     2161 caacggcgac gaggcggtgt tcacagagca cggtttcctc gtctacaaga tcgtcacgct
     2221 cggcaatggc gcaaagccgg ttgtcgtgaa gtgcagggag caccgcacgg atgtggacgc
     2281 catcatcgcc gcgctgacgc cgcggacgaa gattgttttc ctcgacaacc cgaacaaccc
     2341 gacgggaacg tacattccct tctcggaggt gcggcgcctg catgcggccc tccgcccgga
     2401 cacgctgctc gtgctcgatt cggcctatgc cgagtacgtg cgccgcaacg actacgaggc
     2461 gggcctcgag cttgtcgcga cgagcgccaa cactgtgatg acgcgcacct tctcgaagat
     2521 ctacgggctc gcggctctgc ggctcggctg ggcctattgc ccggcccaca tcgctgatgt
     2581 gctcaatcgc atccgaggac cgttcaatgt gacggcgcca gcgattgcgg cgggtgtcgc
     2641 ggcgataaac gacagcgcgc atgtcgatgc gtcgctcgcc cacaacgaca aatggctccc
     2701 ctgggtgacg gcggagctcg agaagctcgg attgaaggtc aaccctagcg tcggcaactt
     2761 catcctgatc catttcccgg ccgatgcggc gcacggggcc gatgccgccg acaagcacct
     2821 gaaatcgagg ggtatcattc tccgccgtgt ggcgggctac ggcctgccgc agtgcctgcg
     2881 catgacgatc ggcacggcnn nnnnnnnnga ggagaatcag aaggttgtgg cggtgctcgg
     2941 cgagttcatg cgaggctgaa atgggcgggg gaccatttcg gagtatttgc catggtattg
     3001 ccgaccctca tcccgcgggc gaagctgttt tcgaacccgg ctcggacctt gcctcaaatc
     3061 agcgcggatg ggcacctgct cgcatggttg tcgcccgtcg aaggtgtgct gaacatctgg
     3121 gttgccccga ttgatgatcc gggtgccgcg cggcaattga cgcgccggac cggccggccc
     3181 gtcatgcact acgactggct gccgaacggg cgccagatcg cctatgtcag cgacgacaac
     3241 ggcgacgaga acctctacgt cgttcttgtc gacgtcgcga ccggcagtga gcgcatcgtc
     3301 acgccgcgaa ccggtgtcgc ggcaagtgtc gcgatggcct cgcccgaccg tccggatgcg
     3361 atgctcgtgc tgctcaacga gcgcgacaag cgctggcacg acgccgtcga gatcgatctc
     3421 gcgacggggg cgcaacgtat cgtcttcgaa aacacgctag gcctgtcgag catgagcttc
     3481 gactggcagc atcggtgccg gctcggggtg cgccagatcg gcggcgggcg ccaggagctc
     3541 ctgcgcctcg atggcgcgcg gcccgaggtc ttgttcgaga ttccgcagcc cgatgccttc
     3601 tcgacggggc cagggggctt caatcgcaac ggtgatcact ggtatctcca ttcctcgatc
     3661 gggcgggatc gaagcgcact cttccgtgtc gatgcactga gcaaccgcca gacgctcctc
     3721 gcagagcacg acacggccga catcagcggg ttgattgccg atccgttgac gttcgacgtc
     3781 atcgccgcgt gcgccgatcc gattcgccgt gagtggatgg cgctcacgga tgcagcgcgc
     3841 gccgatctcg caatactgaa agcggcgctg ccagggctcg agctgtctgt cgtcagccag
     3901 agcaacgtcg gccgctgggt catcgggtcg tatgcgggtg accagccgat ggtttatcat
     3961 ctctatgatc gcaccgagcg gacggtgaca aagctcttcg gtgtccgccc ggagctcgag
     4021 gcctattcgc tcgcgccgac acacggccag atcatcaagt cgcgcgacgg cctcgacctc
     4081 gtctgctacc tcaccctgcc ggccagtgag acgggcgagc gcccgaaggc gccgctgccg
     4141 atggtgctgc acgtgcacgg cggcccctgg catcgcgact actacggcta caaccgcgac
     4201 aaccaatggc tcgccaaccg cggctatgcc gtgttggcgg tcaactaccg ggcttcgacc
     4261 gggttcggga aggccttcat caacgctggc gacaaggagc atgccggcaa gatgcacgac
     4321 gacctcatcg atgccgtcga ttgggccgtt cgcgagggca tcgccaggcg cgacaaggtc
     4381 gcgatcatgg gcgcctccta tggcggctat gcgacgctcg tcgggctcac gtttacgccc
     4441 gatgtgttct gctgcggggt ttcggtcgtc ggcatctcga acctcgtcac gatgctcgag
     4501 aacatcccgc cgtactggga aggcttcaac gagcagatgt tcggacgcta tgccgatccg
     4561 cgcacggagg aaggacgcgc ctggctacgg tcgcgttcgc cgctctcgcg cattgacagg
     4621 atttcgaagc cgctgctcct cggtcacggc gccaatgacg tgcgttgcaa gcagatggaa
     4681 tcggatcaga tcgtcgatgc catgacggca cgcggtctgc cggccaccta tgtggtctac
     4741 accgacgagg gccacggctt ctatcgcccg gagaacaaca tgtccttcaa tgcgataacg
     4801 gaagccttcc ttgcccgcca tcttggcggc cgcatggagc cgatcggtag cgatctcgac
     4861 ggctcgagcc tcatcgtgcc gacgggggct gagtcgattg aaggcctcat cgacaagctt
     4921 ccgccggagc ggcgcctacg acaagcgagc tgagaaggac gtaatgctaa aggattcgaa
     4981 ggaccccatg tttgggcggg tggcactgat cggcatcggc ctgatcggat cgtcgatcag
     5041 tcatgcctgc cggcgcggca agctcgcaca gaccatcgtc ggtgctgcca agacggaggc
     5101 gacgcgggcg gcagccctgc ggcttggcct cgtcgatacg gcatacgcga gctcggccga
     5161 tgcggtgggc ggcgccgacc tcgtcatcat gtgtgtgccg gtcggcgcct gcggcgcagt
     5221 cgcagccgag atcgggcctc acctaaggcc gggcgcgatc ttgacggatg tcggttccgt
     5281 aaagggcgcg atcgtgcgcg attgccagcc gcatgtgccg aagggcgtgc acttcattcc
     5341 ggggcacccg atcgctggca cggagcactc ggggcccgaa tcgggttttg cgacactctt
     5401 cgacaaccgc tggtgcatcc tcactcctcc ggcaggcgcc gacgcggccg cggtcgacag
     5461 gctcgccgcc ttctggcggg cgcttggctc gaacgtcgag accatggacg ccgaccatca
     5521 cgacctcgtg ctcggcatca ccagccacgt gccgcacctc atcgccttca acatcgtcaa
     5581 cacggcggcc cacctcgagc gggtgacgga cagtgaggtg atcaagttct cggccggcgg
     5641 ctttcgtgat ttcacgcgca tcgcggcgtc cgacccgacc atgtggcgcg acgtgttcct
     5701 caacaatcgc gaagccgtgc tcgagatgct cgcccgcttc acggaggatt tgatccacct
     5761 gcagcgctgc attcggtttg gtgacggcga cacgatgttc cgccttttca ccgaggctag
     5821 agccatccgc cggggcatca tccaggcggg acaggatacg gcggctcctg atttcggccg
     5881 gggccaggtc accgctccac caagacgatc ctgagctcgc ggcgcgatgg atgggatcgt
     5941 attccttgcc gtgctcggcg ccgcgctctg ccatgccggc tggaatgcgc ttctgaagct
     6001 cgacctcgag ccgttgctcg cgattacgct gatctcggtt gcctgcggtg tcgtcgcgat
     6061 ccccatgctg ccgtttgcgg gcctgctgca tccgagtgcg tggccctaca tcgcgggctc
     6121 gctcgcactg catctcgtct attacacggc gctcgccgag gcctacaaga cgggcgatct
     6181 cggccaagtc tatccgattg cccgcggttc cgcgccgctg atgacttcga ttggcgcagc
     6241 gttcattctc gacgagcggc tcggaccggt cggctggtcc ggcatcgtcc ttctcgcctc
     6301 gggcatcctt acgctgtcgc tcggtggagc ccggtcgctg cccgccaacc cgcggacggt
     6361 cggctttgcg ctgctgactg cggtctcgat cgcgctctac accctcgtcg acggtgccgg
     6421 tgtacggacc tcgcaaaacg ttgcagccta tgtcgcctgg ctgttctttc tcgacggcct
     6481 gatgatggcc gtgttcggct actggtatgc gcgccagcgg ttcatcgagg ccgcgcgcgc
     6541 aaagtggcgg ctcgttgcct tcggtggcgt gctgtcgacg ctcgcctacg caattgcgct
     6601 ctgggcgatg acgcgggcgc cgattggcct cgtcgcggcg ctgcgcgaga cgagtgtgct
     6661 gttcgccgcg gcgatcggca ttgtctttct cggcgagcgg ctgagccgcg cccgcattat
     6721 cgccgccctg ctcgtggtgg ccggattgat gctgatccgc ctcaagtaga cgcggcaggc
     6781 gggatgatga ggtcgaaggc cgggccgccg gcgcggatct cgagccgcgc gtggcgctcg
     6841 ggatcgccgt ccacctgagc cgaaatcggc cgctcggccg tgactacgac acgcgtcgcc
     6901 ggcaaacagg cgacgcctcg ctccgatctc gcgagatccg ggaggcgccc cgtcgccagt
     6961 gccaggagga ccttcgcaag ggtgccgcgc cgctggtcgg tcagcagcac ggcgacgagc
     7021 cccggcttgg acagctcggt gcggtcggtc aggtggaagc gtccgccgta gcggctcgca
     7081 ttggtgacga cgacccagcc gcaaggctga catctcccat cgacctcgac ggtcagcgga
     7141 tcgagcgggg ccgcgatcgc gcgggcgatg gcgccgccat aagcgagctt gccgaggcgg
     7201 cgcttggcac cgtgatgcaa ggcgccggcg acgcgaccgt cgaagcctgc gccgacccag
     7261 aggaagaacg ggtggccgtt gacgatgccg ccggtgatgc gcgtcgtggc gccgtcgag
//
LOCUS       DAORNH010000233         7292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7557_length_7292_cov_2.425038, whole genome shotgun sequence.
ACCESSION   DAORNH010000233 DAORNH010000000
VERSION     DAORNH010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7557_length_7292_cov_2.425038"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..697)
                     /locus_tag="PK264_19115"
     CDS             complement(89..697)
                     /locus_tag="PK264_19115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRD78020.1"
                     /translation="MRAFHSAFPDVDLRFQLISGSLKGPIGNVDLGMRRTEPGVKDAW
                     TTEFAPEIILPVCSPSYLSANGPIESNSTSASHVLIQLSDSEIDWRSMLPRSAHTRIP
                     HGNWIEFSDYAVVIQTAMSGQGIALGWVSAISRGLLDGTLVPASRLRKVTGNTFSLVA
                     PRGRALPKIVPEIRNWMIAEMRQDLDRLEPMLGAASLGGRRD"
     gene            645..800
                     /locus_tag="PK264_19120"
     CDS             645..800
                     /locus_tag="PK264_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78021.1"
                     /translation="MSWNRRSTSGNALWNARIRGTNQCVTKAVDSDSSTTCFLAFCRL
                     ISVSVSS"
     gene            1138..2760
                     /locus_tag="PK264_19125"
     CDS             1138..2760
                     /locus_tag="PK264_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD78022.1"
                     /translation="MTSGKWPSRPTISSADRALIEQAMRRGASRRELLGLLAAGGASL
                     AMGGAILGSATDALAQAPRKGGRIKVAGSAISPQDTVDPAKQSYSVDYARCAMFYNGL
                     TSLDGSLTPQPELAESFTSDKAKVWTFKLRKGVTFHDGKPLTAADVVYSLNRHKDKAT
                     GSRANALAVQMEEIKAVGTDTVVITLTSPNADLPVILGTWHFLIIKEGTTEFKTAIGT
                     GPYKVKEFTPGIRTIGVRNENYWKPGKPYLDEIELIGIPDETARVNAMISGDVQLIAG
                     INPRSIRLIEGSPGFKVFETKAGYYTDLIMRQDIDPVKNPDFQMAVKHLQNREEIRSA
                     VFRNFAVVGNDQPIDPSNRYYCKEIAQRPFDPDKAKFPFQKSGIGNTALPLYASTAAP
                     GSIEMGVQLQEAAQKIGMNIDLRKVPPEGYWSNIWIKQPFAYGAINPRPSADILLTLF
                     FKHDAAWNESKWKNDKFEGLLIAARSESDEAKRKQHYCDIQAIIKDNCGLGIPVFITL
                     LDAHSEKLRGLSPIPLGNLMGNAFAEHIWLAA"
     assembly_gap    2972..3020
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3021..3788
                     /locus_tag="PK264_19130"
     CDS             <3021..3788
                     /locus_tag="PK264_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78023.1"
                     /translation="PPHERYLGWILGLMRGDPGISLVNQLPVAQQIGQRLPNSLMLAG
                     FTAMLAVPLALFIGVTTAMRQGSRYDRIMSTLTVSMVSVPEFLIASVAVLILAVKLRW
                     LPALSTVSDSMSWLQLAKALAMPVLTLTLIVVAQMARLTRAALVNTLRLPFVEMAILK
                     GASPTRVVLLHALPNAIGPIANAIALSLSSLLGGVVVVETIFNYPGLASLMLNAVALR
                     DIPIVLACAMIFSTSYLVLVTIADVLAILSNPRLRKA"
     gene            3805..4659
                     /locus_tag="PK264_19135"
     CDS             3805..4659
                     /locus_tag="PK264_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78024.1"
                     /translation="MTAADNTAPVVQTRRKRPLLAVVGMTVVGFWLLMAVIGPYVAPY
                     TAAKRVDLDFFGPMSLAFPLGTDYLGRDMLSLVLYGARYTIGLALAATVLACIAGTSL
                     GLLAAAVGGWLDAILSRALDALVSIPSLLFSLVVITIFKSSIPILIAAAAVIYTPGAY
                     RIARSLAVNVNATDFVTVARARGESTRYVMFTEILPNITGPILADFGLRFVFVILLLS
                     RLSFLGLGVQPPLADWGSLVRQNLAGFVYGAPAVIAPAVAIASLTISVNMIIDSLQLR
                     RPSAMEGQ"
     gene            4661..6541
                     /locus_tag="PK264_19140"
     CDS             4661..6541
                     /locus_tag="PK264_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78025.1"
                     /translation="MAPESDIAPVSDLAPVVDVRGLIVTGRDDAGREAQIVSDVSFSI
                     ASGEVVALIGESGSGKSTIALSLMGYARPGCRIVGGSVAINGDDVLSLSPKERARLRG
                     RRVAYVAQSAAAAFNPSKRILTQIVEGARIHGTFTLAEARKRAIELFAALLLPDPERF
                     GDRFPHQVSGGQLQRAMLAMALFNEPEVIILDEPTTALDVTTQIEVLEAFRKVVRERR
                     TTALYVSHDLAVVAQVADRIIVLQRGRMQETGSTAKLIEAPQHDYTRSLLEAADPAAR
                     VSRGPRGAEAGGVALLVVKGVTAGYGALDQRGRPEATVLHDVDITITTGSAVGVIGES
                     GSGKSTLARVIAGLLPAAQGTIELGGRRLAARAEDRTLADLRDIQIVFQMADTALNPS
                     HTIARILGRPATLFDGLAGAALEKRVHELLDMVQLPAHFAERRPAELSGGQKQRVNLA
                     RALAASPKLILCDEVTSSLDTVVGAAVLKLLSELRSRLGLSYLFISHDLSTVRAVCDE
                     VVVLYAGRRVQATAGRATAGRPVHPYVDLLVSSIPELHVGWLDALLERQPSESRHPAA
                     GRSKAASSGCAFFARCGTARPGTCDREPPPERRLASGAVIACHAGEADLAAAQAARHT
                     SL"
     gene            6560..>7292
                     /locus_tag="PK264_19145"
     CDS             6560..>7292
                     /locus_tag="PK264_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methylproline demethylase"
                     /protein_id="HRD78026.1"
                     /translation="MTTTTLTQGAAPTSTRRVRDPLLEPLQIKKLRFKNRVMSTSHAS
                     TLDDGGIPRERYQRYHEEKAKGGIALTMFGGSSMVAPDSSWGGGQVNVASDDVIPHLR
                     EFSERIHAHGANIMVQISHLGRRADALGWNWLPTMGPSPIRERRHRGFPREMDDHDIT
                     RVIRDYAEAAKRCKEGGLDGCETVTGGHLIGQFLSPRTNKRTDGFGGSTENRARFGLM
                     VHEAIRRAVGDDWLVGIRFVVNEGHE"
BASE COUNT         1189 a         2527 c         2307 g         1220 t
ORIGIN      
        1 ctcgatcagg atgcgtcgcc gtcgcgatcg cccggcgccg acctcccgtt gcctccgggt
       61 tgaacgacgc tatcgcgatc gtcttcattc agtcgcgccg gccgcccagg ctcgccgcac
      121 cgagcatcgg ctcgagccgg tcgaggtcct ggcgcatctc ggcgatcatc cagttgcgga
      181 tctcgggcac gatctttggc agcgcgcgcc cgcgtggagc gaccaggctg aaggtgttgc
      241 cagtcacctt gcgcaggcgc gaggccggca ccagcgtgcc atcgaggaga ccgcgggaga
      301 ttgcgctcac ccagccgagc gcgatgccct ggccgctcat cgcggtctgg atgacgacgg
      361 cgtagtccga gaattcgatc cagttgccgt gcgggatcct ggtgtgggcc gagcgcggca
      421 gcatcgagcg ccagtcgatc tcgctgtcgg agagctggat gaggacatgc gaggcgctcg
      481 tcgagttgct ctcgatcggg ccattggccg aaagatacga tggactgcag acgggcagga
      541 tgatctcggg cgcgaattcc gtcgtccaag cgtccttcac accgggctcc gtccgccgca
      601 tgccaaggtc gacgttgccg atcggccctt tcagcgagcc ggaaatgagc tggaaccgca
      661 gatcgacgtc ggggaatgcg ctatggaacg cgcgcatccg gggcacgaac cagtgcgtga
      721 cgaaggccgt cgacagcgac agctcgacga cgtgcttcct cgccttctgc cggctgatct
      781 cggtgagcgt ctcgtcgtaa gtgtgcggta gagtttctcg ccgctggtcg tgagtgtgag
      841 acccgacggg ctgcgcaaga atagtgtcgc gtcgagtgcc gcttcgagct cggcaatgtt
      901 gcggctgacc gagggctgcg tgagattgaa ctcgcgggcc gccgcgctgc agctgccgag
      961 gcgagccgct gcctcgaaaa tcagcagcac acgcggcgag cggataaggc ggccgaggag
     1021 catggtcata cggcgagcgt atatcccccc gatgcattgt caagcgtacg cgctcccaca
     1081 gctgtgccac actcgcgtgg tcaagacgtc cacatgcgac ggcaagggag acgcgacatg
     1141 acctctggca aatggccaag ccgaccgaca atcagcagcg ccgacagggc attgatcgag
     1201 caggcgatga ggcggggggc gagccgccgc gagctgctgg gactgctcgc ggccggaggc
     1261 gcgagcctcg ccatgggcgg cgcgatcctc ggctccgcaa cggatgcact ggcgcaagcg
     1321 ccgcgaaagg gcggccgcat caaggtcgcc ggcagcgcga tctcgccgca ggacacggtc
     1381 gatccggcga agcagtccta ctcggtcgac tatgcgcgct gcgccatgtt ctacaacggc
     1441 ttgacgtcgc tcgacggctc gctgacgccg caacccgagc tcgccgagag cttcacgagc
     1501 gacaaggcca aggtgtggac cttcaagctc cgcaaaggcg tcaccttcca cgacggcaag
     1561 ccgctcacgg ccgccgacgt cgtctactcg ctcaatcgcc acaaggacaa ggcgaccggt
     1621 tcgcgcgcca atgcgctcgc cgtgcagatg gaggagatca aggcggtcgg caccgacacg
     1681 gtcgtgatca cgctcacgag cccgaatgcc gacctccctg tgatcctcgg cacctggcac
     1741 ttcctgatca tcaaggaggg cacgaccgag ttcaagacgg cgatcggcac cggcccctac
     1801 aaggtgaagg agttcacgcc cggcatccgc acgatcggcg tacgcaacga gaactattgg
     1861 aagccgggta agccgtacct cgacgagatc gagctcatcg gcatccccga cgagacggca
     1921 cgcgtcaacg cgatgatctc aggcgacgtg cagctgatcg ccggcatcaa cccgcgctcg
     1981 attcgcctga tcgagggttc gcccggcttc aaggtgttcg agaccaaggc cggctactat
     2041 accgacctca tcatgcgcca ggacatcgat ccggtgaaga acccggactt ccagatggcg
     2101 gtgaagcacc tccagaaccg cgaggagatc cgctcggcgg tgttccgcaa tttcgcggtc
     2161 gtcggcaacg accagccgat cgatccctcg aaccgctact actgcaagga gatcgcgcag
     2221 cgcccgttcg atccggacaa ggcgaagttc cccttccaga agtcggggat cggcaacaca
     2281 gcgctgccgc tctatgcctc gaccgcggct ccgggctcca tcgagatggg tgttcagctg
     2341 caggaggccg cccagaagat cggcatgaac atcgacctcc gcaaggtgcc gcccgagggc
     2401 tactggtcca acatttggat caagcagccg ttcgcctacg gcgccatcaa cccgcggccg
     2461 agcgccgaca tcctgctcac gctcttcttc aagcacgatg cggcctggaa cgagagcaag
     2521 tggaagaacg acaaattcga gggcctgctg attgcggcgc gctcggagtc cgacgaagcc
     2581 aagcgcaagc agcactattg cgacatccag gccatcatca aggacaactg cggcctcggc
     2641 attccggtgt ttatcacgct gctcgatgcg cactcggaga agctgcgcgg gctctcgccg
     2701 atcccgctcg gcaacctcat gggcaatgcc ttcgccgagc acatctggct cgcggcgtag
     2761 gtcatggctc ggcgggtccg atgacggtcg gacccgtcgc ctcgcaaaca tcgttcaatt
     2821 catcgattgg acagactcac gcatgaacgt gacgattgcc ggcctcatcg tgcgccgcat
     2881 cggcacgagc ctcatgatcc tcgcgctgat gacggtgatt gtcttcagcg cgacgctctt
     2941 cctgccgggc gacgttgtcg aggagacgct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn ccgccgcacg agcgctatct tggctggatc ctagggctca
     3061 tgcgcggcga tcccggcatc tcgctcgtca atcagctgcc ggttgcccag cagatcggtc
     3121 agcggctgcc gaactccctc atgcttgccg gcttcacagc catgctcgcc gtgccgctcg
     3181 ccctcttcat cggcgtcacg acggcgatgc gccagggctc gcgctacgac cgcatcatga
     3241 gtacgctcac cgtatccatg gtgtcggtgc cggagtttct gatcgcctcg gtcgcggtac
     3301 tgatcctcgc cgtcaagttg cgctggctgc cggcgctctc gaccgtttcc gacagtatga
     3361 gctggctgca actcgccaag gcgctcgcca tgccggtgct gacgctcacg ctgatcgtcg
     3421 tggcgcagat ggcgcggctc acgcgcgccg ccctcgtcaa tacgctccgc cttcccttcg
     3481 tcgagatggc catcctgaaa ggggcgagcc cgacgcgcgt ggtcctcctg catgccctgc
     3541 cgaacgcgat cggtccgatt gccaacgcaa tcgcgctcag tctctcctcg ctgctcggcg
     3601 gcgtcgtcgt ggtcgagacg atcttcaact atccgggcct cgcgagcctc atgctgaacg
     3661 cggtggcgct gcgcgacatt ccgatcgtgc tcgcctgtgc catgatcttc tcgacgagct
     3721 atctcgtgct cgtcacgatc gcagacgtgc tcgcaatcct gtcgaacccg cgattgagga
     3781 aggcctgatg cgagagccgc tgccatgacc gccgccgaca atacggcacc cgtcgtccag
     3841 acgcgccgca agcggccgct gctcgctgtc gtcggcatga cggtcgtcgg cttctggctg
     3901 ctgatggccg tgatcgggcc ttatgtcgcc ccctacacgg ccgcaaagcg cgtcgatctc
     3961 gacttcttcg ggccgatgag cctggccttc ccgctcggca ccgactatct cggccgcgac
     4021 atgttgagcc tcgtactcta cggcgcgcgc tacacgatcg gccttgccct cgcggcgacc
     4081 gtgctcgcct gcattgccgg gacttcgctc ggcctcctcg cggccgccgt cggcggttgg
     4141 ctcgatgcga tcctgagccg cgccctcgat gcactcgtct cgatcccgag ccttctcttc
     4201 agcctcgtcg tcatcaccat cttcaagagc tcgattccga tcctgatcgc cgccgccgcc
     4261 gtcatctata cgcccggcgc ctaccgcatc gcccgctcgc ttgccgtcaa cgtcaacgcc
     4321 accgacttcg tcacggtcgc ccgcgcccgc ggcgagagca cgcgctatgt gatgttcacc
     4381 gagattctgc cgaacatcac cggtccgatc ctcgctgatt tcggactgcg cttcgtgttc
     4441 gtcatcctgc tcctgagccg gctgagcttc ctcgggctcg gcgtgcagcc gccgctcgcc
     4501 gactggggct cgctcgttcg ccagaacctc gcgggcttcg tctacggcgc gccggccgtg
     4561 atcgcgcccg ccgtcgccat cgcctctctg acaatcagcg tcaacatgat catcgacagc
     4621 ctgcagctcc gccgcccgag cgcgatggag ggccagtgac atggcacccg agagtgacat
     4681 agcgccagtg agtgacttgg cacccgtggt cgacgtgcga ggcctgatcg tcaccggccg
     4741 cgacgatgcc ggccgtgagg cgcagatcgt ctccgacgtg tcgttctcga tcgcctcggg
     4801 cgaggtggtg gcgctgatcg gcgagtccgg ctcgggtaag tcgacgatcg ccctctcact
     4861 catgggctac gcgcggcccg gctgccggat cgtcggcggc agcgtcgcga tcaacggcga
     4921 cgacgttctc tcgctctcgc cgaaggagcg cgcgcgtctc cgcggacggc gcgtcgccta
     4981 tgtcgcgcag agtgccgccg ccgccttcaa tccctcgaag cgcatcctca cgcagatcgt
     5041 cgagggcgcg cgcatacacg gcacgttcac gctggcggag gcgcgcaagc gcgccatcga
     5101 gctcttcgca gcactcctgc tccccgatcc cgagcgcttc ggcgatcgct ttccccacca
     5161 ggtctccggt ggccagctgc agcgcgcgat gctcgccatg gcgctcttca acgagcccga
     5221 ggtgatcatc ctcgacgagc cgacgacggc gctcgacgtg acgacgcaga tcgaggtgct
     5281 cgaggcgttc cgcaaggtcg tgcgcgagcg gcggacgacg gcgctctacg tctcgcacga
     5341 tctcgccgtc gtcgcgcagg tcgccgaccg catcatcgtc ctgcagcgcg ggcgcatgca
     5401 ggagacgggg tcaaccgcga agctcatcga ggcgccgcag cacgattata cgcggagcct
     5461 gctggaggcc gccgatcctg ccgcgcgcgt ttcgcgcggt ccgcgcggcg cggaagccgg
     5521 aggcgtggcc ctgctcgtcg tcaagggcgt gacggcgggc tacggcgcgc tcgatcagcg
     5581 cggccgccct gaggcgacgg tgctgcacga cgtcgatatc actatcacca cgggctcggc
     5641 cgtgggcgtc atcggcgaat cgggctcggg aaagagcacg ctcgctcgtg tcatcgcagg
     5701 attgttgccg gcagcgcagg gcacgatcga gcttggcgga cgtcgccttg ccgctcgcgc
     5761 cgaagaccgc acgcttgccg acctccgcga catccagatc gtcttccaga tggcggatac
     5821 cgccctcaat ccctcgcaca cgatcgcccg catcctcggg cggccggcca cgctgttcga
     5881 tggcctcgcc ggcgccgccc tcgagaagcg cgtgcacgag ctcctcgaca tggtgcagtt
     5941 gccggcgcat ttcgccgagc gacggccggc tgaactttcg ggcggccaga agcagcgcgt
     6001 caatctcgcc cgcgcgctgg cggcgagccc caagctcatc ctctgcgatg aggtgacgtc
     6061 gagcctcgat acggttgtcg gcgccgcggt gctaaagctc ctttcggagc tcagaagccg
     6121 gctcggcctc agctatctct tcatcagcca cgatctctcg accgtgcgcg ccgtctgcga
     6181 cgaggtcgtc gtgctctatg ccgggcgccg cgtgcaggcg acggcgggtc gcgcgacggc
     6241 cggacgcccg gtacacccgt acgtcgatct cctcgtctca tcgatcccgg aactgcatgt
     6301 cggctggctc gatgcgcttc tcgagcgcca gccgagtgag agccgccatc cggcagctgg
     6361 acggagcaag gcggccagca gtggctgcgc cttcttcgcg cgctgcggga ccgcgcgtcc
     6421 aggaacctgt gaccgcgagc ccccgcccga gcgccggctc gcctccggag ccgtcatcgc
     6481 ctgtcacgcc ggcgaggccg atctcgccgc ggcgcaggcc gcacgccaca cctcgctttg
     6541 acttggaatg gagaccgcca tgaccaccac cacgctgacc cagggcgccg ccccgacctc
     6601 cacgcgccgc gtgcgcgatc cgctgctcga gccgctgcag atcaagaagc tgcgcttcaa
     6661 gaaccgcgtc atgtcgacga gccacgcgtc gacgctcgac gatggcggca tcccgcgcga
     6721 gcgctaccag cgctatcacg aagagaaggc gaagggcggc attgcgctca ccatgttcgg
     6781 cggctcgtcg atggtcgcgc cggattcgag ctggggcggc ggacaggtca acgttgccag
     6841 cgacgacgtc ataccgcacc tgcgcgagtt ctccgagcgc atccacgctc acggcgccaa
     6901 tatcatggtg cagatttccc atctcggccg gcgcgccgac gcgctcggct ggaactggct
     6961 gccgaccatg ggaccctcgc ccatccgcga gcgccggcat cgcggcttcc cgcgcgagat
     7021 ggacgatcat gacatcaccc gcgtgatccg cgattatgcg gaggcggcga agcgctgcaa
     7081 ggaaggcggc ctcgacggct gcgagacggt caccggcggc cacctgatcg ggcagttcct
     7141 ctcgccgcgg acgaacaagc gcaccgatgg gttcggcggc tcgaccgaga accgcgcgcg
     7201 cttcggcctc atggtgcacg aggcaatccg cagggccgtc ggcgatgact ggctcgtcgg
     7261 catccgcttc gtggtcaacg agggccacga gg
//
LOCUS       DAORNH010000234         7284 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7567_length_7284_cov_2.402269, whole genome shotgun sequence.
ACCESSION   DAORNH010000234 DAORNH010000000
VERSION     DAORNH010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7567_length_7284_cov_2.402269"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>309)
                     /locus_tag="PK264_19150"
     CDS             complement(9..>309)
                     /locus_tag="PK264_19150"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRD78027.1"
                     /translation="TSRQLKAAMIAPTGPDVAQAIRAGHADVGIATRAVATAAGVDFI
                     PLLIERFDILTRQRDSYRGPMATFLAMLRAPEFAARAAELGGLDVSEAGRIRWAP"
     assembly_gap    310..381
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<382..901)
                     /locus_tag="PK264_19155"
     CDS             complement(<382..901)
                     /locus_tag="PK264_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD78028.1"
                     /translation="MQAHGGLVLLTTAEAASYLRIKERKLYELVSEGAIPCTKVTGKW
                     LFPRADLDRWLLAGMARPLGVVPAEPPPIVGGSHDPLLQWALAESRAGLAILPEGSET
                     GYRRFLAGAVIAAAIHFHDVDAPEADANVAVVSHEPTLYDAVLIGFATREQGLLVASG
                     NPRSIASLEDAAR"
     gene            complement(1187..1453)
                     /locus_tag="PK264_19160"
     CDS             complement(1187..1453)
                     /locus_tag="PK264_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78029.1"
                     /translation="MHTLQMTLAGLALLALLAVAAKVLGSPYWTMTRAARLFIPLWLV
                     IAIANMLIGILSAGIPFLTELAVLAVVFGVPAAAAWYLMRREQA"
     gene            complement(1453..2589)
                     /locus_tag="PK264_19165"
     CDS             complement(1453..2589)
                     /locus_tag="PK264_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HRD78030.1"
                     /translation="MVELFFDLVFVFAITQLSHALLADVSVANGLRIGVLLLAVWWAW
                     IYTSWTTNWLDPDQTPVRFMLFVLMLSGLVMSAAIPRAFTDRGLAFALAFVVIQVGRT
                     AFACWALRRHNEGQFRNFARIGCWLVLSGVFWIVGGLASGNLRVTCWMIALAIEYMAP
                     AFGFWTPHFGRSTTRDWDIDPAHMAERCALFVIIALGESLLVTGATFAAAAWKPTTVM
                     AFVVAVVGTIAMWWVYFNIGADEATHRFKSAEDPGRIARLAYTYLHLPIVAGIVVAAV
                     ADELVLAHPYGATDNKTAATLVGGPVLFIGGTLLFKRATAGRWPLSHLVGLGLLLLLL
                     LTVPLFWPLGVAALSAGILVSIAIWETVSLQSVRKELTMSLGEH"
     gene            complement(2812..3162)
                     /locus_tag="PK264_19170"
     CDS             complement(2812..3162)
                     /locus_tag="PK264_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78031.1"
                     /translation="MLPRIILLLVQLAAGWSLAPHIVRFLPKLGSLDIFVVAAVFAIL
                     VVMIGFIGSLVLKEVGPPSSQTMTTVLVVALVFAALTLVPQVVSAVSSVTKGQVPKLA
                     YPLIGAVLGYFARR"
     gene            complement(3214..3621)
                     /locus_tag="PK264_19175"
     CDS             complement(3214..3621)
                     /locus_tag="PK264_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78032.1"
                     /translation="MINGLIIAICQLVGTYLLTPLVSLGSGRLATILNIKDGLLQLLL
                     LAALIGVVVWAIGLVLSIVLGARYPLFGALLFAVALSVAFVAVTKFVPGIQAQLKPLI
                     ERAAAATRLTISDMNIAGVAAFIGYWINMLIRR"
     gene            3971..5131
                     /locus_tag="PK264_19180"
     CDS             3971..5131
                     /locus_tag="PK264_19180"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRD78033.1"
                     /translation="MTAIPLTVLTGFLGSGKTTLLKALLSHPDMAGTGVIINEFGEVG
                     LDDALVEKADEDTVLLASGCVCCTVRGDLVEALDRLYGRMVEGEVPPIRRVVLETTGL
                     ADPAPIVHTLMTEEVVYRYFQLDGVVTTVDGEHGLAELEREYEPAKQVAIADRIVITK
                     PDRADGAALARLEAEVRRLNPAAQVFKAVKGDISPDKLFGLGAHEVVLTGTDASRWLA
                     ADAYAKPAAAAHVHAHHHHHHDGPCLDDTCIDPTHNHHGHDHACGPDCHDPAHGHDHR
                     HLHGIASFCLTFTDPLDGQQLSDGLKLLTQLHGERLLRVKGIVDIKGQRKPFVLHGVQ
                     HVFYPPETLGDWPPGPRRSRIVFITKDLSEPEVMKLLTPFVTVQPHWPDARA"
     gene            complement(5141..5932)
                     /locus_tag="PK264_19185"
     CDS             complement(5141..5932)
                     /locus_tag="PK264_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRD78034.1"
                     /translation="MDNVAIRTAAERIWQLWSLGDTLAHLPPELSPSSRAEGYAIQAD
                     LDRLSRESRVGWKIAATSAAGQKHIGVSGPIAGRIFRARVHAAGATVSIAKNRMRVAE
                     PEFAFRFARSLAARDDPYSVDEVLAAVESLHLALELPDSRFDDFAAVGEATLIADNAC
                     AHDLILGAAVTADWRPFDLSDFAVSATVARRYERAGNARNVLGDPRLALTWIANELSD
                     LGIGIAAGEFVTTGTSVPPLEIIPGDQVRADFGVLGSISATIAAG"
     gene            complement(5944..>6377)
                     /locus_tag="PK264_19190"
     CDS             complement(5944..>6377)
                     /locus_tag="PK264_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRD78035.1"
                     /translation="LLEWAEVHGNLYGTPRARVEDELARGRDVLFDIDWQGARQLAAG
                     AAADIVRIFILPPSGQALAERLEKRAKDAPEVVARRLAGAPREIEHWSDYDYVIVNGD
                     LEESFRGLVAIVAAERLKRSRQTGLALLAKSIIADLGKLRA"
     assembly_gap    6378..6400
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6401..6561)
                     /locus_tag="PK264_19195"
     CDS             complement(<6401..6561)
                     /locus_tag="PK264_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRD78036.1"
                     /translation="MLVLSSPSGAGKTTLARRLLDSDPAISLSVSLTTRPPRDGEVDG
                     RDYTFVDRSA"
     gene            complement(6602..>7284)
                     /locus_tag="PK264_19200"
     CDS             complement(6602..>7284)
                     /locus_tag="PK264_19200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRD78037.1"
                     /translation="TIDRATAPAEVRVNAAVLRAVTESALRLTVTPGISPPTADGLLA
                     LRGVLEQAEPVETDSEARSAAMLASLDEATTKLAEARAQEGARLADVIRTQLDQIERL
                     AGEVERSPSRTPEAIKARLVQQVARIVGASSAIDPARLHQEVALLAVKADIDEELARL
                     RSHLVAARELLGAKEPVGRKLDFLAQEFNREANTLCSKSNDVEVTRLGVELKVVIDQM
                     REQVQNIE"
BASE COUNT         1318 a         2316 c         2421 g         1134 t
ORIGIN      
        1 gcccgcgatc acggcgccca gcggatgcgg ccagcctcgc tgacatcgag tccgccgagc
       61 tcggcggcgc gtgcggcgaa ctcgggcgcc cgcagcatcg cgaggaaggt cgccatgggg
      121 ccgcgatagc tgtcgcgctg gcgcgtcagg atgtcgaagc gctcgatgag cagcgggatg
      181 aagtcgacgc cggccgccgt cgcgaccgct cgcgtggcga taccgacgtc ggcgtggccg
      241 gctcggatcg cttgcgcaac gtcggggccg gtcggtgcga tcattgctgc tttgagttga
      301 cgcgatgtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn ngccgtgccg cgtcctcgag gcttgcgatc gacctcggat
      421 tgccgctggc gacgaggagg ccctgctcgc gcgtggcaaa gccgatcagc accgcgtcat
      481 agagggtcgg ctcgtgcgag acgacggcga cgttggcatc ggcctcgggc gcgtccacgt
      541 cgtggaaatg gatggcggcc gcgatcacgg cgccggcgag gaagcgccgg tagccggttt
      601 cgctcccctc gggcaggatc gcgagaccgg cgcggctttc cgcaagcgcc cactgcagca
      661 ggggatcgtg actgccgccg acgatcggtg gcggctcggc cgggacgacg ccgagcggtc
      721 gcgccatgcc ggcgagcagc cagcggtcga ggtcggcgcg agggaagagc cacttccccg
      781 tgaccttcgt gcaggggatc gcgccttcgg agacgagctc gtagagcttg cgctccttga
      841 tgcgcagata gctggcggct tccgctgtcg tgagcagtac gagaccgccg tgcgcctgca
      901 tttttctggc tctcgctcgt gccgtcgatg atccatcagg gatcacgccc cctcccgcgg
      961 cgtcaagcga gacgaggcgc cggctgtcaa ggccgcgaac gctgcgcgga tgcctgccac
     1021 gtcgccggtt tctgaggcag ccggtagtgc acgcacgacc tgcgtcgggt gtccctgctc
     1081 gctaccgccc tggccgacag tgctgagcga gaacgtcgat atgccttgac cacgcagatc
     1141 aattgacgcc ggaacatgat cagcggaaac gatgctccct gagggctcaa gcctgctcac
     1201 gtcgcatgag ataccacgca gctgctgccg gtactccgaa tacgacagcc agcactgcaa
     1261 gctcggtaag gaacgggatg cccgcactca gaatgccgat aagcatgttt gcgattgcga
     1321 tgaccagcca gagagggatg aagagccgag cggcccgcgt cattgtccaa taggggctac
     1381 ccaacacctt ggcagcaaca gcaagcagcg ccagcagcgc cagtcccgct aacgtcattt
     1441 gcagtgtgtg catcagtgtt ctcccaagct cattgtcagt tcctttctaa cgctctgcag
     1501 cgaaaccgtt tcccagatcg caatcgaaac gagaatgccg gcggaaagtg ctgccacccc
     1561 gagcggccaa aaaagcggaa cagtcagaag aaggaggagc aggaggccga ggccaacgag
     1621 gtgcgacaac ggccatcgac ctgcggttgc ccgcttgaac agcagcgtac cgccgatgaa
     1681 cagcaccggc ccgccgacga gcgttgcggc ggtcttgttg tcggtcgccc catacggatg
     1741 ggctagcacc aattcatcgg caaccgctgc caccacgatt ccggccacga tcggcagatg
     1801 caggtaggtg taggcgagac gtgcaatcct gcccgggtct tccgccgact tgaagcggtg
     1861 cgtggcctca tctgcaccga tattgaaata cacccaccac atcgcaatcg taccgactac
     1921 tgccaccacg aacgccatca ccgtggtcgg cttccaggcg gcagccgcaa acgtcgcgcc
     1981 ggtcacgaga agcgactcac cgagcgcgat gattacgaag agtgcgcacc gctccgccat
     2041 atgagccggg tcgatgtccc aatcgcgggt cgttgagcga ccgaagtgtg gtgtccagaa
     2101 gccgaaggct ggcgccatgt actcgatggc aagcgcaatc atccagcaag tcacgcgcaa
     2161 gttgccgctg gcgaggccgc cgacgatcca gaacacacca gacaggacga gccagcagcc
     2221 gatgcgggcg aagttgcgaa attggccttc gttgtggcgc cgcagtgccc agcatgcgaa
     2281 agccgtgcga cctacttgaa tgacaacaaa ggcgagggcg aaagccaagc ctcgatcggt
     2341 gaacgcgcgc ggaatggctg ccgacatgac gaggccggac agcatgagca cgaacagcat
     2401 gaaccgaacg ggtgtctgat cggggtcaag ccagtttgtg gtccacgagg tgtagatcca
     2461 ggcccaccag accgcgagca gcagcacgcc gatgcggagc ccgttcgcga cactcacatc
     2521 ggccaataaa gcatgtgaga gctgcgtgat ggcgaagaca aagacgaggt cgaagaacag
     2581 ctcgaccatc gtcaccttct ggtgcccgcc ggctgccggc cggcgcaagt cctgggaagg
     2641 cccgaagtga agcatgaccc ctctcttgct cggtaggtca taaaactcag cagcaaacga
     2701 gggtcagttc caagcatgac ggcgcggaca agcaactgcc tgaagcgctc gttggagata
     2761 ctgtgacgaa cctcatttga cccttcggcc catggtgctc taccaggcaa ctcaacgccg
     2821 agcaaaatag ccgaggacgg cgccgatcag cgggtaggcg agcttcggca cctggccctt
     2881 ggtgacggac gacacggcgg acacgacctg cgggacgagg gtcaaggccg cgaacacgag
     2941 cgcgacaacc agaaccgtcg tcatggtctg gctcgacggc gggccgacct ctttcaggac
     3001 cagcgaaccg atgaagccga tcatgacgac tagtatcgcg aacacggcgg ccaccacgaa
     3061 aatgtcgagc gagccgagct tcggcaggaa cctgacgatg tgcggcgcaa gcgaccagcc
     3121 ggcggcgagc tgcacgagca gcaggatgat gcgtggcaac atggcgaccg gtggctccgg
     3181 gtgatcattc ggggggacgc gggaggcgca ggatcagcgg cggatgagca tgttgatcca
     3241 gtagccgatg aaggcggcaa cgccggcaat gttcatgtcg gagattgtga gccgcgtggc
     3301 cgccgctgcg cgctctatga gcggcttcag ctgcgcctgg atgccaggca cgaacttcgt
     3361 cacggcgacg aaggcgaccg agagggcaac ggcgaacagc agcgccccga acagcgggta
     3421 gcgcgccccg agcacgattg aaagcaccag gccgatcgcc cagacgacca cgccgataag
     3481 ggctgccagc aaaaggagct gcagcagtcc gtccttgatg ttgagaatcg tcgcgagccg
     3541 gccggagccg aggctcacaa gcggcgtcag caggtaggtt ccgacgagct gacagatcgc
     3601 gataatcagg ccgttgatca cgcaagcctc ccttggatgt gtttcgaggc aacagctgat
     3661 cccatctacc acggggcctt agccgatgcg agcagatcga gcagtggctc ctgggcaatt
     3721 cagatctcag ttctggttcc ccgggcggag cgcgaaagcg cgaagagcgg agatccttac
     3781 ctctcgcgcg ggttaagacc ccggctctcg ctccgctcgg ccggggaacc agtgcggggg
     3841 agtaggtttg acacctcgat ttttgaatgg ccttgcttcc tccccgtctc ctttcgcccg
     3901 cgacggtgga cagggcgtgc gtggtgttgc acgcttgccc ggttcgcaat cgacccgcgc
     3961 aggcagagcc atgaccgcca ttccactcac cgtgctcacg ggctttctcg gctcgggcaa
     4021 gacgacactc ctgaaggcgc tgctcagtca ccctgatatg gcgggaacgg gcgtcatcat
     4081 caacgagttc ggcgaggtcg ggctcgacga tgcgctcgtc gagaaggcag acgaggatac
     4141 agtgctgctc gcttcgggct gcgtgtgctg tacggtgcgc ggcgatctcg tcgaggcgct
     4201 tgaccggctc tatgggcgca tggtcgaggg cgaggtgccg ccgatccgtc gcgtggtgct
     4261 cgagacgacc gggctcgccg atccggcgcc gatcgtacac acgctcatga ccgaggaggt
     4321 cgtctaccgc tacttccagc tcgatggcgt ggtgacgacc gtcgacggtg agcacgggct
     4381 tgccgagctc gagcgcgagt acgagcctgc aaagcaggtg gcgatcgctg accggattgt
     4441 catcaccaaa ccggaccggg cggacggcgc tgcgctcgcc cgcctcgagg ccgaggtgcg
     4501 tcggctcaat ccggcggcac aggtcttcaa ggccgtgaag ggcgacatct cgcccgacaa
     4561 gctcttcggc ctcggcgctc acgaggtcgt gctgacggga acggatgcca gccgctggct
     4621 cgcggccgac gcctatgcca agcccgccgc cgcagcgcac gtgcacgcgc accaccatca
     4681 ccaccacgat gggccgtgcc tcgacgacac gtgcatcgat ccgacgcaca atcatcacgg
     4741 ccacgatcat gcctgcgggc cggactgcca cgatccggcg catggccacg atcatcgcca
     4801 cctacacggc atcgcctcgt tctgcctgac cttcaccgac ccgctcgacg gccagcagct
     4861 ctcggatggc ttgaagctgc tcacccagct ccacggcgag cggctgctgc gcgtgaaggg
     4921 tatcgttgac atcaaggggc agcgaaagcc gttcgtgctc cacggcgtgc agcacgtgtt
     4981 ctatccgccg gagacgctcg gcgactggcc ccccggcccg agacgctcgc gcattgtctt
     5041 cattacgaag gacttgtccg agcccgaggt tatgaagctc ctgacgccgt tcgtgacggt
     5101 gcagccgcac tggcccgacg cgcgcgcatg agcctgccgc tcagccggcg gcgatcgtcg
     5161 cgctgatcga gccgaggacg ccgaagtcgg cgcggacctg atcgccgggg atgatctcga
     5221 gcggcggtac ggacgtgccc gtcgtcacaa actcgcctgc agcgatgcca atgccgagat
     5281 cagacagctc gttcgcgatc caggtgagtg cgagccgcgg gtcgccgagg acgttgcggg
     5341 cattgccagc gcgctcgtac cgcctggcga ccgtggcgct cacggcgaag tccgagagat
     5401 cgaacggccg ccagtcggcg gtcacggcgg cgccgaggat caggtcgtgt gcgcaggcgt
     5461 tgtcggcgat gagcgtcgcc tcgccgaccg cggcaaagtc gtcaaagcgg ctgtcgggca
     5521 gctcgagcgc gagatgcagg ctctccaccg cggcgaggac ctcgtcgacc gaatacggat
     5581 cgtctcgcgc cgcaagcgac cgcgcgaagc ggaaggcgaa ctcgggttcg gcaacgcgca
     5641 tgcggttttt cgcgatcgag acggtggcgc cggctgcatg cacgcgggcg cgaaagatgc
     5701 gtccagcgat cggtccgctg accccgatgt gcttctggcc ggcggcgctc gtcgcggcga
     5761 tcttccagcc gacgcggctc tctctcgaga gccggtcgag atcggcctgg atggcgtaac
     5821 cctctgcgcg cgacgatggc gacagctcgg gtggcaaatg agcaagcgtg tcgccaaggc
     5881 tccacagctg ccagatgcgc tccgccgcgg tccggatggc gacgttgtcc atgcgagtat
     5941 ccgctatgcc ctgagcttgc cgagatcggc gatgatcgac ttggcgagaa gtgcgaggcc
     6001 cgtctggcgc gaccgcttca gccgctcggc ggcgacgatt gcgacaagac cacggaagct
     6061 ttcctcgagg tcgccgttga cgatgacgta atcgtaatcg gaccagtgct cgatctcgcg
     6121 cggcgctccg gcgaggcgac gtgccacgac ctccggcgcg tccttggcgc gcttctcgag
     6181 ccgctcggcg agtgcctggc ccgatggtgg caggatgaag atgcgaacga tgtccgccgc
     6241 cgcgccggcg gcgagctggc gcgcaccctg ccagtcgata tcgaaaagta cgtcgcggcc
     6301 ccgcgcgagc tcgtcctcga cgcgggcacg gggcgtgccg tagagattgc cgtgcacttc
     6361 ggcccattcg aggaggcnnn nnnnnnnnnn nnnnnnnnnn gcgctccggt cgacgaaggt
     6421 gtagtcgcgg ccgtcgacct cgccgtcgcg cggtggccgc gtcgtcagcg acaccgagag
     6481 ggagatggcg ggatcgctgt cgagcaggcg gcgcgccagc gtggtcttgc ccgcgcccga
     6541 aggggaagag agcaccagca tgattccgcg gcgctcagcc ggatgcgaat ttgcggctgt
     6601 gtcactcaat gttctgcacc tgctcacgca tctgatcgat caccaccttg agctcgacgc
     6661 caagccgcgt cacctctacg tcgttggact tcgagcagag cgtgtttgct tcccggttga
     6721 actcctgggc aaggaagtcg agcttgcggc cgacgggctc cttcgcgccg agaagctcgc
     6781 gggcggcgac cagatgcgac ctgagccgtg caagctcctc gtcgatgtcg gccttgacgg
     6841 cgaggagcgc cacctcctgg tgtaggcgcg ccggatcgat ggcgctcgag gcgccaacga
     6901 tcctcgcgac ctgctggacg aggcgggcct tgatcgcctc aggcgtccgc gacggcgacc
     6961 gctcgacctc gccggcgagc cgctcgatct ggtcgagctg cgtgcggatg acatcggcga
     7021 ggcgggcgcc ttcctgagcc cgcgcctcgg cgagcttcgt cgtcgcctcg tcgagacttg
     7081 ccagcatggc ggccgagcgc gcctcggaat cggtttcgac cggctcggcc tgctcgagaa
     7141 cgccacgcag cgccaggaga ccgtccgctg tcggcgggga aatgcccggg gtcacggtga
     7201 gacggagcgc gctctcggtc acggcgcgta gcacggctgc attgacacgg acctccgccg
     7261 gtgccgtggc gcggtcgatg gtga
//
LOCUS       DAORNH010000235         7267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7593_length_7267_cov_2.578896, whole genome shotgun sequence.
ACCESSION   DAORNH010000235 DAORNH010000000
VERSION     DAORNH010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7593_length_7267_cov_2.578896"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PK264_19205"
     CDS             <1..985
                     /locus_tag="PK264_19205"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HRD78038.1"
                     /translation="AATVKPAPAAPVAPTATAPAPSTGGMTASPPPPPPPVASPKITT
                     VKDDAPVHDCDRLAASPEDPQRKVTAVPRLDVRDAAISSCREAVARYPDTPRFMFQLG
                     RALLLRGSLEESTRWFKQSAEGRYPIGMAYYGYAFSDGTGVTKDVVEAARWLKRAAEA
                     GNPMAMSSYAFLLTDAVGVQRNDFEAREWYRKSALAGHRDGMSGLAFMLDNGRGGSKD
                     LRGAAEWHRKAAEAGSTASMEALAGMLHAGDGVTKNIPEAVNWIIRAIELGDKTTKEN
                     IANKSTEWNDEFRRAFQRKLTDLGVYSGAIDGNFGSGTKAAIEKIFGRKKE"
     gene            complement(1351..2130)
                     /locus_tag="PK264_19210"
     CDS             complement(1351..2130)
                     /locus_tag="PK264_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRD78039.1"
                     /translation="MQQRIESKAELRSNPVEWQARVDLAAAHRIAVMHGFHEGIFNHL
                     TLSVPGKVDRYYQIPFGLHWSEVTASCFMEVGWDGQVLRGEGEVEASCYAIHAPIHRR
                     LGARGAAVFHTHMPYASALTRLADPKLKMIGQTEVLVSTEVAYDMDYQGPACDPTEGE
                     RLVEIIGDKKVLFMGSHGIATVAPSVAQAYDLLYYVERAAQVQIYAMWTGQKLLEMPP
                     AIVAQTKAEVGGQKFLHGKPYDYHFRALKRILDRREPDYAD"
     gene            2484..3182
                     /gene="purQ"
                     /locus_tag="PK264_19215"
     CDS             2484..3182
                     /gene="purQ"
                     /locus_tag="PK264_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRD78040.1"
                     /translation="MRSAVIVFPGTNRERDVADVLADVSGKASTLVWHGETALPATDL
                     IVLPGGFSFGDYLRTGAMAVHSPVMREVIRRAKAGTPVLGICNGFQTLCESGLLPGVL
                     MRNASLKFVCRDVFLRVETTRTLYTSRYAKGEVAKVIVAHGDGNYFADEATLDRLEGE
                     GRVAFRYTDRDGKTTSEANPNGSQRNIAGITDATGRILGMMPHPENAAERILGSTDGR
                     KLFESIVDGVMAKA"
     gene            3289..3843
                     /locus_tag="PK264_19220"
     CDS             3289..3843
                     /locus_tag="PK264_19220"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRD78041.1"
                     /translation="MPYHLFDTAFGTCAIAWSDAGLTRVQLPEATREATEARMRRAGT
                     NAAEPPPPAFAADAVTALQSYFSRAPVSLDELRLDESIVSTFNASIYRALRAVPRGAT
                     VTYGDLAIRIGQPGAARAVGMAMGRNPWPVIVPCHRVLARGGKMGGFSAPGGTATKER
                     LLALEDVTVGDPVLPGLFEQRRPS"
     gene            3840..>4673
                     /locus_tag="PK264_19225"
     CDS             3840..>4673
                     /locus_tag="PK264_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRD78042.1"
                     /translation="MTRRRPDALSLAEARRIAIAAQGAHQSAERGSATRANVGSLIDK
                     LGVLQIDSVNVLARAHTLPIYARLGVYDVADLDDLAYGGRRRRLFEYWGHAASLMPVS
                     LQPLMRWRMDAARTGTGVYAGLVRFAEAHAPLIRDLRREIGERGPSAVGDFEAHANRK
                     AGWWGWSDAKAALEWLFWTGELTTATRRSTFERIYDIPERVLPGPVLAAPTPSEADAK
                     RALIEHAARALGIGTEACLRDYFRLGSAAAKPAIRDLVEAGRLREVTVEGWSASAYIH
                     AD"
     assembly_gap    4674..4731
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4732..5045
                     /locus_tag="PK264_19230"
     CDS             <4732..5045
                     /locus_tag="PK264_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRD78043.1"
                     /translation="RAEALFGAHIRIELYTPKHKRTHGYYVMPFLFGDRVVGRVDLKA
                     DRAVERLQVLSAHCEPGIAAETFVSALADEIRLMARWLDLPRIEVSRTGDAATALQRI
                     V"
     gene            complement(5097..6059)
                     /locus_tag="PK264_19235"
     CDS             complement(5097..6059)
                     /locus_tag="PK264_19235"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRD78044.1"
                     /translation="MREVTYNAAAVEGVAEEMRRDPKTFYMSTDSIMSLVKEFGTTRV
                     RATPIAESAVTGIAIGAAGSGYRPIVDWRQITFAFVAMDQIVNQAAKIHYMFGGQRKF
                     PILFRAAVGGGTRLAAQHCQSPYSMFMNVAGLKMILPSTPADLKGLIKSAIRDDNPVI
                     SFESNRLMQLKGPVPEGDDHLVPIGKADIKRSGRDVTIVAVAWMVHEALAAAETLEKD
                     GISAEVVDPRTLVPLDAEAIRASVRKTGRLIVADEAGPTAGFSAEVAAVVTEDAATFA
                     KLKGPVRRVCALNVPIPYAPVLEDHVFPNRHRIIAAVREAVEGN"
     gene            complement(6071..>6758)
                     /locus_tag="PK264_19240"
     CDS             complement(6071..>6758)
                     /locus_tag="PK264_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD78045.1"
                     /translation="KGKGGSMHIADFGIGMLGANGIVAGGMSIVTGAALAAQFDGKGR
                     VAVSFFGDGASNAGPFHECMNIAAAWKLPIVYVCENNRYAAQTSAQSTHAMEDVAGRA
                     HGYGIPGVVVDGNDIFAVHQAAYRAVERARAGEGPTLIEAKTFRWRAHTERVGQPDFR
                     TKEEIAAWIEKDPIARLVERLKAQGQLADADLAAMKGEVSGAIERACAFAEKSPFPKP
                     EQATDDVFAA"
     assembly_gap    6759..6790
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6791..7025)
                     /locus_tag="PK264_19245"
     CDS             complement(<6791..7025)
                     /locus_tag="PK264_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRD78046.1"
                     /translation="MNLSPDRQRAMLRQMLTIRQFEERASADYHAGKIYGVVHCYIGE
                     EAVAVGVCGALEAGDQIIFLRRVEPGAAGRSFGI"
BASE COUNT         1165 a         2406 c         2410 g         1196 t
ORIGIN      
        1 agcggcgacc gtgaagccgg cacccgctgc accggtcgcg ccgacagcca cggcccctgc
       61 cccgtcgacc ggcggcatga cggcatcgcc tccaccgcct ccgccacctg tcgcctcgcc
      121 aaagatcacc accgtgaagg acgacgcacc tgtccacgat tgcgaccggc tggcggcttc
      181 gccggaggat ccacagagga aggtaacagc ggtgccgcgg ctagacgtca gagatgccgc
      241 gatttcgtcg tgtcgggagg ccgttgcccg ctatcccgac actccacgtt tcatgttcca
      301 gcttggcagg gcactgctgc ttagaggctc tctcgaagag tccacgcgct ggttcaagca
      361 gagcgctgaa ggtcggtacc ccatcggcat ggcctactac ggttacgcct tcagtgatgg
      421 aaccggcgtg accaaagacg tggtcgaagc agcgcgctgg ctgaagcgtg ctgcagaagc
      481 cggaaatcct atggcaatgt cgtcgtacgc gttcttgttg acggatgcgg tcggcgtgca
      541 gcggaacgat ttcgaagcgc gcgagtggta tcgaaagtcc gcgttggccg gccatcgcga
      601 tggcatgagt ggattggcct tcatgctcga caatggtcgc ggaggctcga aggacctgcg
      661 cggggcggca gaatggcacc gcaaggccgc ggaggccggc tcgaccgcat ccatggaggc
      721 gctagccggg atgctccatg caggtgacgg cgtcaccaag aatatccccg aggccgtaaa
      781 ctggatcatt cgcgcgattg agctcggcga caagacaacc aaagagaaca tcgccaacaa
      841 gagcaccgaa tggaacgacg agttccgtcg cgcgttccag cggaagctca ccgatctcgg
      901 cgtctactcc ggtgcgatcg acgggaactt cggctccggc accaaggcgg ccatcgagaa
      961 gattttcggg cgcaagaagg agtgaggtgc cgacgatccg tgtccggcgc gaccgacgcg
     1021 caccacgacg cgacggtcgt gggaagattg actgtcgggt ctcgtacgaa tgccggcgcc
     1081 ggcacgtagg ttgggctgag ctcggcgcga agcccaacaa gtccagtcgc atccgaggct
     1141 gttgagcttc gctccgctca gcaccaacct acgtggccgg tgcgccaccc gtgcctgctc
     1201 tgcgaggagg ccgcatgggg ccgccgcggt tcagcacgtg ctccgacacg gaccggcccc
     1261 gacgatcgct ggggtccggc cagatcgcag cgcgcgatgc tgctgtgggg ctgtcgggct
     1321 cccacaaccg cgcttgatgc agctggagtg tcaatccgcg tagtcgggct cgcggcggtc
     1381 gaggatgcgc ttcagggcgc ggaagtggta gtcgtagggc ttgccgtgca ggaatttctg
     1441 gccaccgacc tcggccttcg tctgcgcgac gatcgcgggc ggcatctcga ggagcttctg
     1501 cccggtccac atcgcgtaga tctgcacctg tgccgcgcgc tcgacgtagt agaggaggtc
     1561 ataggcctgc gcgacggacg gcgcgacggt cgcaatgccg tggctgccca tgaacagcac
     1621 cttcttgtcg ccaatgatct cgacgaggcg ctcgccttcg gtcggatcac acgccggacc
     1681 ctgatagtcc atgtcgtagg cgacttcggt cgacaccagg acctcggtct ggccgatcat
     1741 cttgagcttc ggatcggcga ggcgggtcaa tgcgctcgcg tagggcatgt gcgtgtggaa
     1801 cacggcggcc ccgcgtgcgc caagccggcg atggatcggc gcgtggatcg catagcacga
     1861 agcttccacc tcgccctcgc cgcgtagcac ctgcccgtcc cagccgacct ccatgaagca
     1921 ggacgccgtg acttccgacc aatggaggcc gaaagggatc tggtagtagc ggtcgacctt
     1981 ccccggcacg gaaagcgtca gatggttgaa gataccttcg tggaagccgt gcatcacggc
     2041 gatgcggtgg gcggcggcga gatcgacgcg cgcctgccac tcgaccggat tggaccggag
     2101 ctcggccttg gattcgatgc gctgctgcat gatggcgtgc ctcgtggctt ggttcgtgct
     2161 gcgcgcggac ggatcgcttg ttgtgatttg gccgaagctt aagcgagaca gcgcgtttgg
     2221 gcaaagcaat ctcgctgagc ggacaacctg acgtcggggt gcttcaggtc tctgttccgg
     2281 ttccccgacc gagcagagcg agccggcaca ccttttcgag gggcaatgat tcggctctcg
     2341 ctcagctcgg caggggcacc gatgatggat tggatcggcc aggctactac agaactgaat
     2401 ggccctgcct gagatttcga ggcgcgcctt gcgccattgc gcctttggct gtaggcggga
     2461 atggcgcgac aaggagatca tccatgcgat cggccgtgat cgtcttcccc gggaccaacc
     2521 gcgagcgcga cgtcgccgat gtgctggccg acgtctcggg caaggcatcg acgctcgtct
     2581 ggcacggcga aacggcgctg ccggcgaccg acctcatcgt gctgccgggc ggcttctcct
     2641 tcggcgacta cctgcgcacc ggcgccatgg ccgtgcactc gcccgtcatg cgcgaggtca
     2701 tcaggcgcgc caaggctggc acacccgtcc tcggcatctg caacggcttc cagacgcttt
     2761 gtgaatcggg cctcctgccg ggcgtgctga tgcgcaatgc ctcgctcaag ttcgtctgcc
     2821 gcgacgtgtt cctccgcgtc gagacaacca ggacgcttta cacctcgcgc tacgccaagg
     2881 gcgaggtcgc caaggtgatc gtcgcgcacg gcgacggcaa ctacttcgcc gacgaagcga
     2941 cgctcgaccg cctcgaaggc gagggccgtg tcgccttccg ctataccgac cgcgacggca
     3001 agaccacctc cgaagcgaac cccaacggct cgcagcgcaa catcgccggc atcacggacg
     3061 cgaccggacg catcctcggc atgatgcccc accccgagaa cgccgccgag cgcatcctcg
     3121 gctccaccga cggccgcaag ctcttcgaga gcatcgtcga cggcgtcatg gcgaaggcgt
     3181 gaaggggcgc gcggggtttg gttggagacg tctcagcgac gggcgcggtg tcgcggaagc
     3241 gctcgcgcgt ttgcacggac tgtcgcgctt ggctaccctg accatcccat gccctatcac
     3301 ctcttcgata ccgcgttcgg gacttgcgcc atcgcatgga gcgatgcggg actgacgcgt
     3361 gtgcagctac ccgaggcgac gcgcgaggcg actgaagcgc ggatgcgccg tgccggcacc
     3421 aatgcggccg aaccgccgcc gccggccttc gctgcggacg ctgtcaccgc gctgcaatcg
     3481 tacttctctc gtgcgccggt ctcgctcgac gaactgcgcc tcgacgagag catcgtatcg
     3541 acattcaacg cgtcgattta ccgggcgctc cgggccgtac cgcgcggcgc gacggtgacg
     3601 tatggagacc tcgcgatccg gatcggccag ccgggcgccg cgcgcgcggt cggcatggcc
     3661 atgggccgca atccgtggcc cgtcatcgtg ccctgccacc gtgtacttgc gcgcggcggc
     3721 aagatgggtg ggttctccgc acccggtgga acggcgacga aggagcggct gctcgcactc
     3781 gaggacgtca ctgtcggcga tcccgtgctg ccgggcctct tcgagcagcg tcgcccgtca
     3841 tgacacggcg gcggcccgac gcactgtcgc tcgccgaggc acggcgcatc gccatcgcgg
     3901 cccagggtgc gcatcagagc gccgagcgag gctcggccac gcgcgcaaat gtgggcagtc
     3961 tgatcgacaa gctcggtgtc ctgcagatcg acagcgtcaa tgtactcgcg cgcgcgcaca
     4021 ccttgccgat ctatgcgcgg ctcggcgtct atgacgtcgc cgacctcgac gaccttgcct
     4081 atggcggcag gcggcgccgc ttgttcgagt attggggcca tgcggcctcg ctgatgcccg
     4141 tcagcctgca accgctgatg cgctggcgca tggatgcggc ccgcaccggc acaggcgtct
     4201 acgccggtct cgtccgcttc gccgaggcgc acgcgccgct catccgcgat ctccgccgcg
     4261 agattgggga acgcggcccg agcgctgtcg gcgatttcga agcgcacgcc aatcgcaagg
     4321 ccgggtggtg gggctggtcc gacgccaagg cggccctcga gtggctcttc tggacgggcg
     4381 agctcacgac ggcgacccgg cgctcgacgt tcgagcgcat ctacgacatc ccggagcgcg
     4441 tgttgcctgg acccgtactg gccgcgccga caccaagcga ggcggatgcc aaacgcgcgt
     4501 tgatcgagca cgcagcacgc gcgcttggca tcggaaccga ggcctgcttg cgcgactatt
     4561 tccgcctcgg ctcggcggcc gcaaagcccg ccattcgtga tctggtcgag gccggccgat
     4621 tgcgcgaagt gacggtcgag ggttggtcag catcggctta tatccatgcg gacnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccgggcag
     4741 aagcgctgtt cggcgcccat atccgcatcg agctctacac accgaagcac aagcggaccc
     4801 acggctacta cgtgatgccg tttctgttcg gcgaccgggt tgtcggccgt gtcgatctca
     4861 aggccgatcg cgcggtggag agactgcaag tcctgtcggc gcattgtgag ccgggcatcg
     4921 cggccgagac gttcgtttca gcgctcgctg acgagatccg cctgatggcc cgctggctcg
     4981 acctgccacg catcgaggtg agccgcacag gtgacgccgc caccgcgcta cagcgcatcg
     5041 tgtgaggcgc cgtggcagtg gggtcgaact caacggaagt tcccctgcgt cgccccctaa
     5101 tttccctcca cggcctcccg cacggccgcg atgatccggt ggcggtttgg aaacacgtgg
     5161 tcttcgagga cgggagcata ggggatcggc acgttgaggg cacagacgcg acgcaccggg
     5221 cctttgagct tggcaaacgt cgccgcatcc tcggtcacga cagccgcgac ctcggcggag
     5281 aagccggctg tcggccccgc ctcgtcggcg acgatcaggc gcccggtctt gcgcacggag
     5341 gcgcggatcg cctcggcatc gagggggacg agcgtgcgcg gatcgacgac ctccgccgag
     5401 atgccgtcct tctcgagcgt ctcggcggcg gcgagcgcct cgtgcaccat ccaggcgacg
     5461 gcgacaatcg tcacgtcgcg cccggaccgc ttgatgtccg ccttgccaat cgggaccagg
     5521 tgatcatcgc cctccggcac agggcccttg agttgcatca gccggttcga ttcgaacgag
     5581 atcaccgggt tgtcgtcgcg gatcgccgac ttgatgaggc ctttgaggtc ggccggcgtc
     5641 gagggcagga tcatcttcag cccggcgacg ttcatgaaca tcgagtaggg gctttggcag
     5701 tgctgcgccg cgagccgcgt gccgccaccg acggcggcgc gaaacaggat cgggaacttg
     5761 cgctggccgc cgaacatgta gtggatcttg gcggcctggt tgacgatctg gtccatggcg
     5821 acgaaggcga acgtgatctg ccgccagtcg acgatcggcc gatagcccga tcccgccgca
     5881 ccgatggcga tgccggtgac cgcgctttcg gcgatcggcg tcgcgcgcac gcgcgtcgtc
     5941 ccgaactcct tgacgagcga catgatgctg tcggtcgaca tgtagaaggt cttcggatcg
     6001 cgccgcatct cctcggcgac cccttcgacg gcggcggcgt tgtaggtgac ttcgcgcatg
     6061 gtcttgctcc tcacgccgcg aacacgtcgt cggtcgcctg ctccggcttc gggaacggac
     6121 tcttctcggc aaaggcacag gcacgctcga ttgccccgga gacttcgccc ttcatcgccg
     6181 cgaggtcggc gtcggcgagc tggccctgcg ccttcagccg ttcgaccaag cgcgcgatag
     6241 ggtccttctc gatccaggcc gcgatctcct ccttggtgcg gaagtcgggt tgcccgacgc
     6301 gctcggtgtg cgcgcgccag cggaaggtct tcgcctcgat gagcgtcggc ccctccccgg
     6361 cgcgcgcccg ctccaccgcg cgataggccg cctgatgcac ggcgaaaatg tcgttgccgt
     6421 cgacgacgac gcccgggatg ccatagccgt gcgcgcgacc ggcaacgtcc tccatcgcat
     6481 gcgtggattg cgccgacgtc tgcgcggcgt agcggttgtt ctcgcagacg tagacgatcg
     6541 gcagcttcca ggcggcggcg atgttcatgc actcgtggaa cggccccgca ttggaggcgc
     6601 cgtcaccgaa gaaggagacc gcgacgcggc ccttgccgtc gaactgggcg gcgagcgccg
     6661 ctcccgtgac gatcgacatg ccgcccgcga cgatgccgtt ggcgccgagc atgccgatgc
     6721 cgaaatcggc gatgtgcatc gagccgccct tgcctttgnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn cgatgccgaa gctccgcccg gccgcgcccg gctccacgcg gcgcaggaag
     6841 atgatctggt cgcccgcctc gagcgcgccg cagaccccga ccgccacagc ctcctcgccg
     6901 atgtagcagt gcacgacgcc gtagatcttt ccggcgtggt agtcggccga tgcccgctcc
     6961 tcgaactgac ggatggtcag catctggcgc agcatggcac gctggcgatc aggggacagg
     7021 ttcatgcgcg tcctccctcg agtacgtaga tgcctcaagc gtgatccagc gcgtcgcgaa
     7081 gcgcaaccgg ccgcggcgcg cgccagccct gcacgccggg aggcgcgggg cctctgcctg
     7141 tcaatgaacg cgatcgattg ccgtcacgcc tgccgccgcc ctgcatcggc gagcttgccg
     7201 ccggaagcgg cgaggcgcgc gatgagcggg gcgagcgccc agcgccggcc atgggctttc
     7261 tcgtagg
//
LOCUS       DAORNH010000236         7231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7657_length_7231_cov_2.729933, whole genome shotgun sequence.
ACCESSION   DAORNH010000236 DAORNH010000000
VERSION     DAORNH010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7657_length_7231_cov_2.729933"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..>312
                     /locus_tag="PK264_19250"
     CDS             104..>312
                     /locus_tag="PK264_19250"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRD78047.1"
                     /translation="MADTPDGQPPREPSDIRPVSIADEMRRSYLDYAMSVIVARALPD
                     VRDGLKPVHRRILYSMIENGYTPDKP"
     assembly_gap    313..442
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            443..1846
                     /locus_tag="PK264_19255"
                     /pseudo
     CDS             443..1846
                     /locus_tag="PK264_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158149.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     assembly_gap    1847..1857
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1858..2888
                     /locus_tag="PK264_19260"
     CDS             <1858..2888
                     /locus_tag="PK264_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRD78048.1"
                     /translation="THTPVLFFSSRGMCYRLKVWRLPAATPQATGKALINLLPLEQGE
                     AITSILPLPEDPAEWARLELMFATRSGNVRRNSLADFESINRNGKIAMKLDDGDQIVD
                     VKIASPDDDVLLTTSRGQCIRFLSKDEVRLFKGRDSDGVRGIRLEDGDRIISMAILRH
                     VEATAEERAAYLKQAAALRRAANAEGEGEPIAEAPEADGEPVGELATISPDRFAELGG
                     KEQFVLTVSERGFGKRSSSYEYRTSGRGGKGIVAMVVNERNGPLVASFPIEDGDDIML
                     VSDGGQLIRCPVGDVRIAGRNTQGVRVFKTDATEKVVSVERIPQDAADGEVPGEAGGE
                     PPPSDDPA"
     gene            complement(2903..3850)
                     /locus_tag="PK264_19265"
     CDS             complement(2903..3850)
                     /locus_tag="PK264_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRD78049.1"
                     /translation="MGAEFVQLSLVPLLIAVLAAVACALPGNFLLLRRQALIGDAISH
                     VALPGIVAAFLITGRVSTWPMMLGAAGSAVVAVTVIEAIRRLGRIEPGAAMGVTFTSM
                     FAAGVLLLEQSDTSRVHLDVEHALYGNLESLVWLGAKGWGSLVDPAALAELPDELPRM
                     AVVATLAALLVVMLWRPLMIATFDEGFAATLGIPVRALGLALVVVSAVAAVAAFSAVG
                     SIIVIAMFICPPAAARLMTHSLARQVGWSLVFATISAIGGYLLAGYGPGWLGFRASGS
                     AAGMIATVSGVILTLACLFGPQRARTMARTSAPGAVPEA"
     gene            complement(3853..5067)
                     /locus_tag="PK264_19270"
     CDS             complement(3853..5067)
                     /locus_tag="PK264_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRD78050.1"
                     /translation="MAALLDALLLRSGYNAALVAIGAALLGSAAGAVGTFLYLRKRAL
                     VADAITHATLPGICLAFMGLTALGLDGRNLSALLAGAAVSAGMGLVAVEWMTRRTRLG
                     EDAAIGAVLSVFFGLGVVLLTVIQAMSQGRQAGLQGFLLGSTAGMLYSEALLIAAGGS
                     AAVVAVVTLRRPMTFVAFDAEHASAIGIDVRAVDFAMMGLVMAITVIGLKVVGLILIV
                     ALLIIPPVSARFWSEQSDRIVIAAAALGAAAGYVGAALSVTAPKLPTGALIVLFSAAL
                     FVLSLLLAPARGVVATLLRRQRFRKAVHLRQGLLSLAHRERILDGLTLSVLRRRGFIR
                     RDGVATERGLKAAAEALRNERRWDIARELHRTELATSELIGLAPIDTVLTRDEVAAID
                     RRLALSGSKGVA"
     gene            complement(5067..5909)
                     /locus_tag="PK264_19275"
     CDS             complement(5067..5909)
                     /locus_tag="PK264_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRD78051.1"
                     /translation="MPQKPLTVMPLTSEAARESTADVPLAIRGLTVAYGDKPAVFSVD
                     ATFKSASLTAIIGPNGAGKSTLLKASLGIVPKVSGQVTCFGKPMARGLHRVAYVPQRA
                     SVDWDFPTRVTDVVAMGLYRELGLLRWLRPSDHRRIEACLDRVGMGAFADRQIGQLSG
                     GQQQRVFLARALAQGADLYLLDEPLAGVDAATESAIIGVLKSLRCEGKTVIAVHHDLS
                     TVREYFDQVFLIDVRRIAEGPTATTFTMANLQTAYGGRLASSLVQELGISGRSAQAIA
                     EGAA"
     gene            complement(5921..6913)
                     /locus_tag="PK264_19280"
     CDS             complement(5921..6913)
                     /locus_tag="PK264_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRD78052.1"
                     /translation="MRIGRRGIVGGLFVVGATVSTALLLMGSSVPANAQARKIGVVAT
                     TGMIADAARQVGGGEVEVTALMGPGVDPHAYRQTRSDIAAMTRADLVLWHGLYLEAQM
                     EELLRDLGRRRKVVAVAESLPKPVLRAHDAYKDRFDPHVWMSPELWAGVVAAVRDGLA
                     AAAPDRRAAFDANAARHLTEIGELAAYAKRILATVPADRRVLITAHDAFNYFGAAYGF
                     EVLGIQGISTESEAGLNRIRELVEIIVARRIGAIFVESSVSDRNVKALIEGSAARGHA
                     VKIGGELFSDAMGEAGTYEGTYIGMIDHNVTTIARALGGEAPERGMGGRLGNKK"
     gene            7036..>7231
                     /locus_tag="PK264_19285"
     CDS             7036..>7231
                     /locus_tag="PK264_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRD78053.1"
                     /translation="MATDRESALLEALRAEGVRITRQRAALLRVIADASDHPDANEIH
                     RRVHQIDSSTSLSTVYRTMSI"
BASE COUNT         1288 a         2425 c         2354 g         1023 t
ORIGIN      
        1 aagaaaaaga tgatccggaa tggcgccggc gtcgtgcaaa ttggcgtcgc gggcgggaat
       61 cggctagaag aatcggcaga tttcccgcaa ggattcgttc gacttggctg acacgcccga
      121 cgggcagccg ccccgcgagc cttccgacat ccgcccggtg tcgatcgccg acgaaatgcg
      181 ccgctcctat ctcgactacg cgatgagcgt gatcgtggcg cgcgcgctgc ccgacgtgcg
      241 cgacgggctg aagcccgtgc accggcgcat cctctattcg atgatcgaga acggctacac
      301 gccggacaag ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnggcagggc aacttcggct cggtcgacgg cgatccgccg
      481 gcggcgatgc gctacaccga ggtccgcctc gccgacgcgg cgatgccgct gctcaccgat
      541 ctcgacaagg acacggtcga cttccagccg aactacgacg gctcggagaa ggagccgacg
      601 gtccttcccg cggcctatcc caatctgctc gtcaacggcg ccggcggcat cgccgtcggc
      661 atggcgacca acatcccgcc gcacaatctc ggcgaggtgg tcgaggcctg cctcgcctat
      721 ctcgacaatc ccgagatgac gatcgaggac ctcgcacaga tcgtgaaggg gcccgatttc
      781 ccgaccggcg cccacatcct cggccgcgcc ggcatcctcg ccgcctacca caagggccgc
      841 ggctcgatca tcatgcgcgc caaggtccac accgaggagg tcagcaagag caaggaggcg
      901 ctgatcgtca cggccattcc ctatcaggtc aacaagaaga cgctgatcga gaagatcgcc
      961 gacctcgttc gcgagaagaa gatcgagggc atctcgaaca tctgggacga gaccaatcgc
     1021 gaaggcatgc gcatcgtcat cgagctgaag cgcgatgcca tgcccgatgt cgtgctcaac
     1081 cagctctggc ggttctcgga cctgcagaca acgttcggcg ccaacatgct cgccttgaac
     1141 ggcggccggc ccgagcagct gacgcttaag gacatgatcg ccgccttcac ggccttccgc
     1201 gaggaggtgg tgacgcggcg gacgaagttc ctgctgcgta agtcgcgcac ccgcgcccat
     1261 gtgctcgtcg gcctcgccat cgccgtcgcc aatatcgacg aggtgatccg cctcatccgc
     1321 acgtccgcga cgccggccgc ggcaaaggag gcgctcaagg ggcgcgactg gccggcgaag
     1381 gacatggcgc cgctgatcga gctcatcgcc gacccgcggc atcgcctcaa tgacgacggc
     1441 acctatcgcc tctccgagga gcaggcgacg gcgatcctcg cgttgacact ctcgcggctg
     1501 acagccctcg gccgagacga gatcggcgac gagctgaaga aaatcgcggc cgagatccgc
     1561 gacttcctcg agattctctc ctcgcgcgcc cgtgtcgtcg agatcatcaa gactgagctc
     1621 ggcctgatca aagacggctt cgccgtgccc cgccgcaccg agatactcga gatcgagggc
     1681 gacctcgagg acgaggacct catccaacgc gaggactgcg tcgttaccgt ctcgatgaag
     1741 ggctacatca agcgcgtgcc gctcggcacc tatcgcgcgc agcggcgcgg tggcaaaggc
     1801 cgttccggca tgtcgacgcg cgacgaggac gtcgtcacgc agatctnnnn nnnnnnncga
     1861 cgcacacgcc cgtactgttc ttctcctcgc gcggcatgtg ctaccgcctc aaggtctggc
     1921 gcctcccggc cgcgacgccg caggcaaccg gcaaggcgct gatcaacctc ctccctctcg
     1981 agcagggcga ggccatcacc tcgatcctgc cgctcccgga ggacccggcc gagtgggcga
     2041 ggctcgagct gatgttcgcg acgcgctccg gtaacgtgcg gcgcaactcg ctcgccgatt
     2101 tcgagagcat caaccgcaat ggcaagatcg ccatgaagct cgacgacggc gaccagatcg
     2161 tcgacgtcaa gatcgcctcg cccgacgacg acgtgcttct gacgacctcg cgcggccagt
     2221 gcatccgctt cctcagcaag gacgaggtgc ggctcttcaa gggccgcgat tcggacgggg
     2281 tgcgtggcat ccgcctcgag gatggcgacc gcatcatctc gatggcgatc ctgcgtcacg
     2341 tcgaggcgac agccgaggag cgggcggcct acctcaagca ggcggccgca ctccgccgcg
     2401 cggccaacgc cgaaggcgaa ggcgagccga tcgccgaggc gcccgaggcc gacggcgagc
     2461 cggtcggcga gcttgccacc atctcgcccg accgctttgc agagctcggc ggcaaggagc
     2521 agttcgtgct caccgtctcc gagcgcggct tcggcaagcg ttcgtcgtcc tacgagtatc
     2581 ggacgagcgg ccgcggcggc aaaggcatcg tcgcaatggt cgtgaacgag cgcaacgggc
     2641 cgctagtcgc ctcgttcccg atcgaggacg gcgacgacat catgctcgtc tcggacggcg
     2701 ggcagctgat ccgctgcccc gtcggcgacg tgcgaatcgc cggccgcaac acgcaaggcg
     2761 tgcgagtctt caagacggac gccaccgaga aagtcgtctc ggtcgagcgc atcccgcagg
     2821 acgcggccga cggcgaagtg ccgggcgaag cgggaggcga gccgccgccg tcagatgacc
     2881 cggcctgata gtcgctgatg gatcaggcct cgggcaccgc gcccggggcc gatgtcctgg
     2941 ccatcgtgcg cgcgcgctgc gggccgaaca ggcaggcgag cgttaggatg acgcccgaga
     3001 ccgttgcgat catgccagct gcgctacccg acgcgcggaa cccgagccat ccgggaccgt
     3061 agcccgcgag cagatagccg ccgatcgcag agatagtggc gaacacgagg ctccagccga
     3121 cctgccgcgc gaggctgtgg gtcataagac gcgctgccgc cggcgggcag atgaacatcg
     3181 cgatcacaat gatcgagccc acagcgctga aggcggcaac ggccgcaact gccgagacga
     3241 cgaccagggc aaggccgagg gcgcgcaccg ggatgccaag cgtcgcggca aagccttcat
     3301 cgaatgtggc gatcatcagg ggccgccaga gcatgacgac aagcagtgcc gcgagcgtcg
     3361 cgaccacggc catgcgcggc agctcgtcgg gcaactcggc cagcgccgcc ggatcgacga
     3421 gcgagcccca acctttggcg ccgagccaga cgaggctctc gaggttgccg tagagcgcgt
     3481 gctcgacgtc gagatgcacg cggctcgtat cgctctgctc gagcagtagc acgccggccg
     3541 cgaacatgga cgtgaacgtc acgcccatcg ccgcgcctgg ttcgatgcgg ccgagccgcc
     3601 ggatcgcctc gatgacggtt actgcaacga cggccgagcc ggcggcaccg agcatcatcg
     3661 gccaggtcga gacgcgcccc gtgatgagga acgcggcgac gatgccgggc aacgccacat
     3721 ggctgatcgc gtcgccgatc agcgcctggc gtctgagcaa taggaaattc ccaggcagtg
     3781 cacaggcgac ggccgcgagg accgcgatca gcagcggcac gaggctcagc tggacgaact
     3841 cggcgcccat ggtcacgcca ctcccttgct gccggagagt gcgagtcggc ggtcgatcgc
     3901 cgccacctcg tcgcgcgtca gaacggtatc gatgggagca aggccgatga gctccgacgt
     3961 cgccagctca gtgcggtgca gttcgcgcgc gatgtcccac cgccgctcgt tgcgcagcgc
     4021 ctcggccgcg gccttcagtc cccgctccgt tgcgacgccg tccctgcgga tgaagccccg
     4081 gcggcgcagc acggaaagtg tcagaccgtc gaggattcgc tcgcggtgtg cgagcgagag
     4141 cagtccctgg cggaggtgga ccgctttgcg gaagcgctga cggcgcagca atgtcgcaac
     4201 cacgccacgc gccggggcga gcagcagcga gagcacgaac aatgctgcac tgaagaggac
     4261 gatgagcgcg cccgtcggca gtttcggcgc cgttaccgac agtgccgcgc cgacgtatcc
     4321 tgcagccgcc ccgagtgctg cggcggcgat gacgatacgg tcgctctgct ccgaccagaa
     4381 gcgtgcggag acgggcggga tgattagcag tgcgacgatc aggatgagcc cgaccacctt
     4441 gagcccgatg accgtgatcg ccatgacgag gcccatcatc gcgaagtcga ccgcacgcac
     4501 gtcgatgccg atggcggacg catgctcggc atcgaaggca acgaatgtca tcgggcgacg
     4561 aagcgtgaca acggcgacta cggccgcgct gccgccagct gcgatcagca gcgcctcgga
     4621 atagagcatg ccggccgtcg acccgagtag gaagccttgc agccccgcct ggcggccttg
     4681 cgacatggcc tggatcaccg tcagcagcac gacgccgagc ccgaagaaca ccgacagcac
     4741 cgcgccgatt gcggcatcct cgccaagccg cgtccgccgc gtcatccact cgacggcaac
     4801 gaggcccatg cccgccgaga cggccgcgcc ggcaaggagg gccgagaggt tgcgcccgtc
     4861 gagaccgagg gccgtcaacc ccatgaatgc gaggcagatg ccgggcagcg tcgcgtgcgt
     4921 gatcgcatcg gcgacgaggg cgcgcttgcg cagatagagg aaggtgccga ccgctcccgc
     4981 cgcgctgccg agcagtgccg caccgattgc gacgagcgcg gcgttgtatc ccgagcgcag
     5041 caggagggcg tcgaggagtg ctgccatcag gcggctccct ccgcgatcgc ttgggcgctc
     5101 cgcccgctga tcccgagctc ctgcaccagc gaggaggcga gccgcccgcc ataggccgtt
     5161 tgcaggttcg ccatcgtgaa ggttgtcgcg gtcggcccct ccgcaatccg gcggacatcg
     5221 atcaggaaga cctgatcgaa gtattcccgc accgtcgaca ggtcatggtg gacggcaatc
     5281 acggttttcc cctcgcagcg cagcgacttg aggacgccga tgatggcgct ctccgtcgca
     5341 gcatcgacac cggcgagcgg ctcgtcgaga aggtagaggt ccgccccttg agcaagtgcg
     5401 cgcgccagga agacgcgctg ctgctggccg cccgagagct gcccgatctg gcgatcagcg
     5461 aacgcgccca tgccgacccg gtcgaggcag gcctcgatgc gccggtgatc ggagggtcgc
     5521 agccatctga gcaggccgag ctcgcggtag aggcccatcg ccacgacatc cgtgacacgg
     5581 gtcgggaaat cccagtccac tgaggcgcgc tgcgggacat acgctacgcg atggagcccc
     5641 cgcgccatcg gcttgccaaa gcatgtcacc tggcccgaca cttttggcac gattccgagc
     5701 gacgccttga gcagcgtcga cttgccagcg ccgttcgggc cgatgatggc ggtcaggctc
     5761 gcggacttga aggttgcatc cacggagaac acggccggct tgtcgccata ggcgaccgtc
     5821 aagcctcgaa tggcgagtgg cacgtcggcg gtgctctcgc gcgcggcttc gctcgtcagc
     5881 ggcatcacgg tgagcggctt ctgcggcatt gcgggctcgc tcacttcttg ttcccgaggc
     5941 gcccgcccat gccccgctcg ggcgcctcgc cgccgagcgc gcgtgcgatg gtcgtcacgt
     6001 tgtggtcgat catgccgatg tatgtcccct cataggtgcc ggcttcgccc atggcatcgg
     6061 agaagagctc gccgccgatc ttgacggcgt ggccgcgcgc agcggacccc tcgatcagcg
     6121 ctttcacgtt gcggtcggag acggaggatt cgacgaaaat cgcgccgatg cgtcgtgcga
     6181 cgatgatctc gacgagttca cggatgcggt tgagacccgc ctcgctctcc gtcgagatgc
     6241 cctggatgcc gagcacctcg aatccatagg cggcaccgaa atagttgaag gcatcgtgcg
     6301 ccgtgatcag gacgcgccgg tccgcgggta ctgtcgcgag gatacgcttg gcgtatgccg
     6361 caagttcacc gatctcggtg aggtggcgcg cggcattggc atcaaaggca gcgcggcggt
     6421 cgggagcagc agcggcgagc ccgtcgcgaa ccgccgccac aacgcccgcc cacagctcgg
     6481 ggctcatcca cacgtgcggg tcgaagcgat ccttgtaggc gtcgtgtgcg cgcagcaccg
     6541 gcttggggag actctcggcg acggccacga ccttccgccg ccggccgaga tcgcggagca
     6601 gctcctccat ctgcgcctcg aggtagaggc cgtgccagag cacgaggtcg gcccgcgtca
     6661 tggcggcgat gtccgaccgc gtctggcggt aggcgtgggg gtcgacgccg ggccccatga
     6721 gcgcggtgac ctcgacttcg ccgccgccga cctgtcgcgc cgcgtcggcg atcatgcccg
     6781 tggtggcgac cacgccaatc ttgcgggcct gagcattggc ggggacggac gagcccataa
     6841 ggagcaacgc cgtcgaaact gtcgcgccaa caacgaatag accaccaacg atgccgcgcc
     6901 ggccaatacg catgcatgcc tccactccga cccgatcaac cacatagggt cgtctggtcg
     6961 cgatgtcaat gcatttagga atgatttgca acaagctcac ttgcaaatgc cacgcaacta
     7021 gggtgttgtg gggccatggc aacggaccgc gaatcagccc tgctcgaagc gctgcgcgcc
     7081 gagggcgtcc gcatcacgcg gcagcgggca gcgctgctgc gcgtgatcgc cgacgcgtcc
     7141 gaccatcccg atgccaacga gatccaccgc cgcgtgcacc agatcgacag ctcgacgtcg
     7201 ctttcgacgg tctatcgcac catgtcgatc c
//
LOCUS       DAORNH010000237         7202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7691_length_7202_cov_2.820904, whole genome shotgun sequence.
ACCESSION   DAORNH010000237 DAORNH010000000
VERSION     DAORNH010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7691_length_7202_cov_2.820904"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PK264_19290"
     CDS             complement(<1..748)
                     /locus_tag="PK264_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78054.1"
                     /translation="MTATGIRERLAKLRSHPVSFLLSINIACAVLGLAQFAFTLSVLL
                     PADFAVIGVLAALGGVVIGFLDVKLADLTTKLYYETDKADLSGRAALLSASLWLHLAA
                     GFCTAAATFVVSLLLARRFLSQEMAAWWAAAMAARIGIAFPVTAAVTFMRLTGDFRAS
                     GWTRLATQIAVTLATVACLRASPSLSGYFAAVGVGAALSIAGSLAVAARMTGKTLGHP
                     LLAPPSISAFARYRAGSFLVGGSLAGLAKLV"
     gene            complement(745..1827)
                     /locus_tag="PK264_19295"
     CDS             complement(745..1827)
                     /locus_tag="PK264_19295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD78055.1"
                     /translation="MLIVNFTLDRQSPVLAWQAAVANALAERVEAVHVFTEWLGEFEP
                     AAGVTFDVMPHRPWGVPRRLGAAWLMRPRLKRILDSIRPDVCFVHMAHEWCYRLGPLL
                     RSRHIPLLLWYAHGSVPWKLKLSSHFADRIITSTPEGFRIETPKKRVIGQAIDTDLFR
                     IAADRVLEPLIVTVGRISRRKRVDLLIEAMAHLRTRPGCGDVRLIAAGPTLTADDRAY
                     MHELEARVTALGLDKAVSLPGPMSQAETARYYESARLHVNVSETGSMDKTVMEALACG
                     CPVLTSNVAFRDELAHFPEMLIASPTAATLAERMADWMSGRSAASPESLRAIVAGRHD
                     LDGWADRLLAELVDLAEGARRARGTP"
     gene            complement(1886..2665)
                     /locus_tag="PK264_19300"
     CDS             complement(1886..2665)
                     /locus_tag="PK264_19300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD78056.1"
                     /translation="MPSKHPLYWTGNVAKARVIARILRDQRSSLKVLDYGAGRGGDWP
                     EVLSERPGIELVCYEPDPGATAALRQTLAGTNARVLDDAQFRATTVGADYIVSFSVLE
                     HVFDRGDYLSEAKRHLAPDGVIHLNYDDGHFRTSLDLDEMRGWRAELANTLQNRLAWL
                     WPMIKRLDRYQARVPRAAIDELIARVGLRTIEDRYENLGAFKQLAKTLPKERQQEFCR
                     FWMATEDELNARFRTEATPVMGDSVNLWREMGSRTLVLRHA"
     gene            complement(2941..>7202)
                     /locus_tag="PK264_19305"
     CDS             complement(2941..>7202)
                     /locus_tag="PK264_19305"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRD78057.1"
                     /translation="IDTNGDGTPDPLVLGTPTAITDIGGNPIGTITVNPNGDVTFDPA
                     PNYTGPVPILTYTPNDSTVDGTPATVAFTITPVNDAPVAQNDTFTTFENSTTGVRLLA
                     NDTDTEGTPLTVTHVDGVAIVPLGSVAVVNGQVEMRADGTIWFVPAAGYSGPVSFTYD
                     VADSDGANSTGTVTGTVLPADYRPDITLDGTTLGTNVTLFQWNHGDPSDLPMDTHDAH
                     NVNSTYVSATGTETANGNLSLTMSGTTQWIDGVTSATLSDAITNGDYISYSFTVADPL
                     PPGQALQLTGFHIYDTGIVNSYDRTIVIADDPSFANARASVPLNSGGTGNASVDINAQ
                     VPIELVPGQTYYIRVYVYGAADPNASAGFDDFTPTFAITQTNDHGTLNAGDPPVAVDP
                     AIANGVHDWAEGDITTLTIVTANIHDGNSEIVQVDGHDYLLGTTDTHTAVAGGTLFDV
                     AYDATTGAFTITNNAGGAMPAGDLQALISSITYRDNQAAPLPGTRTLTFTVTDSLGQA
                     SSAAVSSITVGNPTVAVDDTANGLEDTSTTIDVLSTDSNADGLALSVTEVNGTSIQAG
                     GSISVANGVVTLNADGTLTFTPVADFNGPATFAYTVSDVNGASAIANVTINVAPVADI
                     ADDTASTPEEGVVTIDVLGNDTFEAPLTVAPPQAIDTDSAFLPPFGFADARAYISGGD
                     TAFPVTIASPGGYPGGIGYAVTSSETGLIQTIRFTVSSDAVGSGIGGIVTDASGNPLA
                     GHWQMLPTGSAAFGDLRYLPMDATSVNSPVTFEYVLDTPTPANTVYLNLAADGFVAAN
                     YQVLSASFTDTRTPLQIVSIDGMPASGGPVTVANGTATLNPDGTIDFNPDVNFAGTTS
                     FTYTVLSGGVMEIATVTVTVTPVNDAPVAQDDAFVTPEDTAHPGTLPVATDVDGGTLT
                     YALGGTSPSHGTVTVNADGTYLYTPAADYHGPDSFTYTVTDGTATVEKTVTVTVTPVN
                     DAPVAQDDAFVTPEDTAHPGTLPVATDVDGGTLTYALGGTSPSHGTVTVNADGTYLYT
                     PSANYHGPDSFTYTVTDGTATVEKTVTVTVTPVNDAPVALDDSFVTSQNSPISFDPRG
                     NDSDPDGDALRITAINGMPIHPGQLVVITGGAISIDSNGRLTFIPSEDFVGPSAFAYT
                     LEDGLGGTAAAQVKIVVIEGGSSAPVVPVLPEEPIELPVRPNQPAASLAVQGAVLDAV
                     NEVASLGSMSGGIRVTGIVIDTVNLVSPLGDLNNGVATVADVAAPLDLPRLKDLWHST
                     SIRAVNAAMGEPERLAGFSLRLGFTGFGMEGSSRSHLIIETLVRDRTLAVQLSSTASI
                     DKRIVDYRVMRADGGPMPGWLDRIGLDLLIGQRPADVDRIALRIIIIFGDGTTELKDV
                     EIDTMSGEIKTLGPGKRADVRLPFMDQPAAGSMISDHSFPR"
BASE COUNT         1252 a         2217 c         2485 g         1248 t
ORIGIN      
        1 cgacgagctt agcgagcccc gcgagcgacc cgccgaccag gaagctgccg gctcggtagc
       61 gggcaaacgc cgagattgac ggtggcgcga gcaggggatg cccgagggtc ttgccggtca
      121 tgcgcgcggc aaccgcgagc gagccggcaa tcgagagcgc ggcaccgacg ccaacggccg
      181 cgaaataccc cgagaggctc gggctcgccc gcaggcacgc gaccgtcgcg agcgtcaccg
      241 cgatctgcgt ggcgagccgc gtccagccgg aggcccggaa gtcgcccgtg agccgcatga
      301 acgtgacggc ggccgtcacc gggaaggcga tgccgatgcg cgccgccatg gccgccgccc
      361 accatgcggc catctcctgc gacaggaagc gacgggcgag cagcagcgag accacaaacg
      421 tcgccgcggc cgtgcagaac cccgctgcca gatgcagcca gaggcttgcc gagaggaggg
      481 ccgcccgacc gctcagatcg gccttgtcag tctcgtagta gagcttcgtc gtgaggtccg
      541 cgagtttcac gtcgaggaag ccgatcacga cgccgccaag tgccgcgaga acgccgatga
      601 cggcgaagtc ggcgggcaga agcacggaca atgtgaacgc gaactgggcg agaccgagca
      661 cggcgcaggc aatgttgatc gagagcagga acgagaccgg gtgcgaacga agctttgcga
      721 gccgctcgcg gatgcctgtc gcggtcacgg cgtccccctg gcgcggcgcg cgccttctgc
      781 gaggtcgacg agctcggcga gcaggcgatc cgcccacccg tcgaggtcat gtcgcccggc
      841 gacgatcgcc cgcaggctct cgggggaggc agcgctgcga cccgacatcc agtccgccat
      901 gcgctcggcg agcgtcgcgg cggtcggact cgcaatcagc atctcgggga agtgggcaag
      961 ctcgtcgcgg aacgctacgt tgctcgtcag cactgggcag ccgcaagcga gcgcctccat
     1021 cacggtctta tccatgctgc cggtctcgct gacgttgacg tgcagccggg ctgactcgta
     1081 gtagcgcgcc gtctcggcct ggctcatcgg ccccggcagc gacacggcct tgtcgagacc
     1141 gagagccgtg acccgtgcct cgagttcgtg catgtaggcc cgatcatccg ccgtcagtgt
     1201 cggacccgcg gcaatgagcc ggacatcccc gcagcccggc cgcgtccgca gatgtgccat
     1261 tgcctcgatc aggagatcga cgcgctttcg acgagagatc cggccgaccg taacgatcag
     1321 cggctccagc acccggtcgg cggcgatacg gaagagatcg gtgtcaatcg cctggccgat
     1381 cacgcgcttc ttcggcgtct cgatgcggaa cccctcgggc gtcgaggtga ttatgcgatc
     1441 ggcgaagtgc gatgagagtt tgagcttcca cggcacagag ccgtgcgcat accagagcag
     1501 cagtgggatg tggcgcgatc ggagcagcgg cccgaggcga tagcaccact cgtgtgccat
     1561 atgcacgaag cagacatcag gccggatgct gtcgaggatg cgcttgagcc gcggccgcat
     1621 cagccaggcc gcgccgagcc ggcgtggaac tccccagggt cgatgcggca tgacgtcgaa
     1681 ggtgacgccg gctgccggct cgaactcgcc gagccactcg gtgaagacgt ggacagcctc
     1741 gacgcgttcg gcgagtgcat tggcgaccgc tgcctgccat gcgagcacgg ggctctggcg
     1801 gtcgagggtg aagttgacga taagcacgcg gatgggtgcc ctgccgcgtc tcccgctcgc
     1861 ggctccgggc cgcccgccgc gcggctcagg catgtctgag gacaagcgta cgggagccca
     1921 tttcgcgcca gagattgacg ctgtcgccca tgacaggcgt tgcctcggtg cggaagcgcg
     1981 cgttgagctc gtcctccgtc gccatccaga agcggcagaa ttcctgctgg cgctccttgg
     2041 gcaaggtctt ggccaactgc ttgaaggcac cgagattctc gtagcgatcc tcgatcgtcc
     2101 tgaggccgac gcgggcgatg agctcatcga tcgccgcgcg cggcacgcga gcctggtagc
     2161 ggtccagccg cttgatcata ggccagagcc aggcgaggcg attctgcagc gtgttcgcga
     2221 gctcggcgcg ccagccgcgc atctcatcga gatcgagcga ggtgcggaaa tgaccgtcgt
     2281 catagttgag atggatgacg ccgtccggcg cgagatgccg ctttgcctcg ctgagatagt
     2341 ccccgcggtc gaagacgtgc tcgagcaccg agaagctcac gatgtagtcg gcaccgacgg
     2401 tggtcgcccg gaactgcgcg tcgtcgagca cgcgggcgtt ggtgccggcg agcgtctggc
     2461 gcagcgccgc agtggcgccc ggatcgggct cgtagcagac gagctcaatg cccgggcgct
     2521 cacttaacac ctccggccag tcgccgccgc gaccggcccc gtaatcgagc actttgagcg
     2581 acgagcgctg gtcgcgcagg atccgcgcaa tgacgcgcgc cttggcaacg tttcccgtcc
     2641 aatagagcgg atgcttcgag ggcatggtca cgctcggagg ctcgatcgaa gtagaggtgg
     2701 gtgcatccgg tgccattgcc tttgcagctc gcaacctgtc tcaggggtcg cgaggagtct
     2761 cgggcgccgt tctatgattg tccagtcaga tcggcaagcc gtcccagccc aaatatctcc
     2821 ctgcccttac agagcgatct agggtcacat ctggcgcgct gcaattttct tgcccgaccc
     2881 gtaccgccga gcctcggcga aggcggacgg agcgggcgat cgggactgaa ccgacgacat
     2941 tcagcgtggg aagctgtggt ccgagatcat ggagccagct gcaggttggt ccatgaatgg
     3001 cagacgcaca tctgcgcgct tgccaggccc gagcgtcttg atttcgccag acatggtgtc
     3061 gatctcgaca tccttcagct ccgtggtccc gtcaccaaag atgatgatga tgcgcaaggc
     3121 aatgcgatcg acatctgctg gtcgctgccc gataagaagg tccaatccga tccgatcgag
     3181 ccatcctggc atgggcccgc cgtcggcgcg catgacgcga tagtcgacaa tgcgcttgtc
     3241 aatgcttgcc gtgctcgaca gttggaccgc cagtgttcgg tcgcgcacga gcgtctcgat
     3301 gatcaagtgc gatcgcgagc tgccttccat gccgaatccg gtaaagccta gccgaagcga
     3361 gaagccagcc aacctctcgg gctcgcccat tgccgcattg acggctcgaa tactggtcga
     3421 gtgccaaagg tccttcaatc gaggcaaatc aagcggcgct gcaacgtcag cgaccgtcgc
     3481 caccccgttg ttgaggtcac cgagcggact gacgaggttg acggtatcga tgacaatgcc
     3541 cgtcacgcgg ataccgcctg acatacttcc gagcgatgcc acttcgttga ccgcgtcgag
     3601 aacggcgcct tgcaccgcga gcgaggccgc gggctggtta ggccgcaccg gcagctcgat
     3661 cggttcttca ggcaggaccg gcaccacagg ggcgctcgag ccaccttcga tgacgacaat
     3721 cttgacttgc gctgcggctg tgccgccaag gccatcttcg agcgtgtacg cgaaggcgga
     3781 gggaccaacg aagtcctcgg acgggatgaa ggtgaggcgc ccattggaat cgatcgagat
     3841 tgcaccgccg gtaatgacca ccagttgccc cggatggatc ggcataccgt tgatcgccgt
     3901 gatgcgcaaa gcatcaccgt cagggtcgct atcgtttccg cgcgggtcga acgagatcgg
     3961 tgaattctgc gatgtgacga agctgtcgtc gagcgccacc ggagcatcat tgaccggcgt
     4021 caccgtgacg gtaacggtct tctcgaccgt ggccgtgccg tcggtcaccg tgtaggtgaa
     4081 gctgtccggc ccgtgatagt tggccgacgg cgtgtagaga taggtgccgt cggcgttgac
     4141 cgtgaccgtg ccatggctcg gcgaggtgcc gccgagggca taggtcagcg tgccgccgtc
     4201 gacgtccgtc gccaccggca gagtgcccgg atgcgcggta tcttccggcg tgacgaacgc
     4261 atcgtcctgc gccaccggcg catcgttgac cggggtcacc gtgacggtga cggtcttctc
     4321 gaccgtggcc gtgccgtcgg tgaccgtgta ggtgaagctg tccggcccgt gatagtcggc
     4381 cgccggcgtg tagagatagg tgccgtcggc gttgaccgtg accgtgccat ggctcggcga
     4441 ggtgccgccg agggcatagg tcagcgtgcc gccgtcgacg tccgtcgcca ccggcagagt
     4501 gcccggatgc gcggtatctt ccggcgtgac gaacgcatcg tcctgcgcca ccggcgcatc
     4561 gttgaccggc gtcaccgtga cggtgacggt ggcgatttcc atgacgccgc cagagaggac
     4621 cgtgtacgtg aagctcgtcg tgccggcgaa gttcacatcc ggattgaagt cgatggtccc
     4681 gtccggattg agcgtggcgg tgccgttggc gaccgtgacc ggtccacccg aagccggcat
     4741 gccgtcgatc gatacgatct gcagcggtgt tctggtgtct gtgaagctcg ccgacagcac
     4801 ctgatagttc gcagcgacga atccgtctgc ggcgaggttc agatagaccg tgttggcggg
     4861 cgtcggcgtg tcgaggacat actcgaatgt gaccggtgag ttgaccgatg tggcgtccat
     4921 ggggagatac cggaggtcgc caaaggcagc ggaacccgta ggcaacatct gccagtggcc
     4981 agccaacggg ttgcccgagg catcggtcac aatgccaccg attccggacc caactgcatc
     5041 ggaactgacc gtgaagcgga tggtctgaat gaggcccgtc tcgctcgagg tgacggcgta
     5101 gccgatgcca ccagggtacc cgccggggga ggcgatcgtc acagggaatg ctgtgtcgcc
     5161 gcccgagatg taagcccgcg cgtcagcgaa gccaaaggga ggcaggaacg cgctgtcggt
     5221 gtcgatggct tgcggcggcg ctaccgtcag cggcgcttcg aacgtgtcgt taccgagaac
     5281 gtcgatggtg acgacgccct cctccggcgt ggaagccgtg tcatcggcga tatccgccac
     5341 cggcgcaaca ttgatcgtga cgttggcgat cgccgaggcg ccgttcacgt ccgacacggt
     5401 ataggcgaag gtcgccgggc cgttgaagtc cgcgaccggc gtgaatgtga gcgtgccgtc
     5461 ggcattgagg gtcacgacgc cgtttgcgac tgagatgctt cccccagcct ggatcgacgt
     5521 gccgttgacc tcagtgaccg acaaagcgag cccatcggcg ttgctgtcgg tcgagagaac
     5581 gtcgatggtg gtcgaagtgt cctcgaggcc attcgccgta tcgtcgacgg caacggtcgg
     5641 attgccgacg gtgatcgagc tcaccgccgc gctcgaggcc tggccgagcg agtcggtgac
     5701 cgtgaacgtc agggtgcgcg ttcccggcag gggcgcagct tgattgtcgc gatacgtgat
     5761 gctgctgatc agcgcctgga ggtcacccgc gggcatagct ccgcccgcat tgttggtgat
     5821 tgtgaaggcg ccggtcgtgg cgtcgtaggc gacgtcgaac aacgttccgc cagcgaccgc
     5881 ggtatgagta tcggtcgtgc ccagcaagta gtcgtggccg tcgacctgta cgatctcgct
     5941 gttgccgtca tggatgttgg cggtgacaat cgtgagcgtg gtgatgtcgc cttcggccca
     6001 gtcatggacg ccattggcga tcgcaggatc gactgcgacc ggaggatcgc cagcgttgag
     6061 cgtgccgtga tcgttggtct gcgtgatcgc gaacgttggc gtgaaatcgt cgaagccggc
     6121 cgaggcgttc ggatccgcgg cgccatagac gtagacgcgg atatagtacg tctgacccgg
     6181 aacaagctcg atcggaacct gcgcattgat gtcgacgctg gcgttccctg tgccgccgga
     6241 gttgagcggc accgaggcgc gcgcgttggc gaacgacgga tcgtcagcga tcacgatggt
     6301 gcggtcgtag gaattgacaa tccccgtatc gtagatgtgg aagccggtga gctgcagcgc
     6361 ttggcccggc ggcaggggat cggcgacggt gaacgaatag gagatgtagt caccgttggt
     6421 gatggcatcg gacaaggttg ccgacgtgac gccgtcgatc cactgcgtgg tgccgctcat
     6481 ggtcagcgac aggttgccat tcgcagtctc ggtgccggtg gcagacacgt acgtggaatt
     6541 gacgttgtga gcgtcgtgcg tatccatcgg caggtcgctc ggatcgccgt ggttccattg
     6601 gaacaacgtg acgttggtcc cgagcgtcgt tccgtcgagc gtgatgtcgg ggcggtagtc
     6661 ggccggcaac accgtgccgg tgacagtccc cgtcgagttg gccccgtcgc tgtcggcaac
     6721 gtcataggtg aacgataccg ggccactgta gccagcggcc ggaacgaacc agatcgtccc
     6781 atccgcacgc atctcgacct ggccgttgac aaccgccacc gagccgagag gcacgatcgc
     6841 cacaccgtcg acgtgcgtga cggtcaacgg ggtaccttct gtatccgtgt cgttggcgag
     6901 cagccgcacg ccggtcgttg aattctcgaa ggtcgtgaat gtgtcgttct gagcgaccgg
     6961 cgcgtcgttg acgggcgtga tcgtgaacgc gaccgtggcg ggcgtgccgt cgaccgtgct
     7021 gtcgttcggc gtgtaggtga gaatcgggac cgggccggtg tagttcggcg ccggatcgaa
     7081 cgtgacgtcg ccgttcgggt tcaccgtgat ggtgccgatc gggttgccac cgatgtcggt
     7141 gatggccgtc ggcgtgccga gcaccagcgg atcgggcgtg ccgtcgccgt tggtgtcgat
     7201 cg
//
LOCUS       DAORNH010000238         7171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7741_length_7171_cov_2.880410, whole genome shotgun sequence.
ACCESSION   DAORNH010000238 DAORNH010000000
VERSION     DAORNH010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7741_length_7171_cov_2.880410"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="PK264_19310"
     CDS             complement(<1..2643)
                     /locus_tag="PK264_19310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD78058.1"
                     /translation="MARKATAKAAKEKRVEALTHEKARRKNIPTAEHQSLAQRQEEMA
                     PVGPIRYSRVYPLKAGTERARDKDIDPQIIWNGARIRLSKEQVRQLQEKGEIEIGDAQ
                     LVWRGKDSQDWSDLVVNAPPLYIQEKVHPKAIIDDLVRQSKKARPDTDDMPDLFADFN
                     GLTDPEARTEFYQHSQHWSNRMILGDSLQVMASLAEREHLRGQVQCIYFDPPYGIKFN
                     SNWQVSTLSRDVKDGKQTDISREPEQVKAFRDTWKDGIHSYLTYLRDRLTVARDLLAD
                     SGSIFVQIGDENLHRVRALMDEVLGTENFVSQISVYKTAGATSVFLSNSQEYILWFAR
                     DIGRMKYRAIYKLKEVGGEGADKYNRLQLGDLQRRPITAEERNSPETVDGRVYRQDNL
                     TSQSVGREKGEGAASWFPVEIDGRTIRPSDKVRWKTNQQGMSRLLRASRVELTGNSLA
                     YVRFLDDFSAVELNNFWEDVGGIQSRADPKIYVVQTPTKAVERCVLMTTDPGDLVLDP
                     TCGSGTTAYVSEQWGRRWTTIDSSRVALALARTRIMSSRFPYYVLSDSREGRTKEQEI
                     SGRIQPDTPTHGDIRQGFVCERAPHIQLSSIARNAEIDVIWETAQTTLEPLRAQLNKA
                     MAKSWEEWEIPREVDPSWSDAAKKLHVEWWEARIERQRKIDASIAKAADVEMLYDRPY
                     EDKSRVRVAGPFTVESLSPHRVVPADEDMLADELKASEGKLRRSKFLAPPTDFADMVL
                     EHLRTAGVHQAEKRDTIKFSSLTGWPGEYIGAEGHFQEGETQRRAAIFLGPEFGTLSR
                     VELVAAAREATEARFDVLIACSFNFDAHASELTRLGPLPILKAKMNPDLHMADELKNT
                     GKGNLFVIFGEPDIDILDQGDETIV"
     gene            complement(2643..3560)
                     /locus_tag="PK264_19315"
     CDS             complement(2643..3560)
                     /locus_tag="PK264_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78059.1"
                     /translation="MSRFGTLDTVPLTEAWAHEAHAFTPWLAENLALLSDAIGIPLEL
                     TDTEVAVESFAADIIARNPQDESVVLIENQFKPSDHGHLGQIMTYLAGLGASTIVWIA
                     PSFREPHLSAIRWLNEHTEEPFAFFAVQIKVVRIGTSPMVPLFDVIERPNHWDRKVQE
                     TARERKAPTTDLGNFRREFWAHMLSRHPSEGDPAKPYAASSRWRDLPELKLAVVQYIA
                     KGGPGIFIRCPRGGDDEAAAELLDPYEQKLESLLDASINAGNPRYLFIKQLRLDATDN
                     ANWDRIADWLHKEADRYEAAIKNVLGGTA"
     gene            complement(3557..6619)
                     /locus_tag="PK264_19320"
     CDS             complement(3557..6619)
                     /locus_tag="PK264_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD78060.1"
                     /translation="MTASFFEAPILNSPYEPPTRHHALDKDGQPLDQPPVDGRRRSEL
                     ITPVPKPRKLLKRAGAAQASLGFSDPAGLSTSEQEYNPTPIINEIRQHIETWRTIPNP
                     ADWGVTPATAQLLKHWRHHNFQGVRPFFCQTEAVETVIWMTEVARREKRYRHLWTHVE
                     GANKDANPELMRLALKMATGSGKTTVMAMLIAWQTVNAVRSPTSTLFTRGFLLIAPGI
                     TIRDRLRVLLPQDADSYYRTRELLPPDMLPDIDRAKIVIANYHAFKRRETMDVSKVGR
                     ALLQGRGEPVVTIETEGQMLQRACGELLAMKNVVVINDEAHHCYREKPGSDDEAEFKG
                     EDKDEAKKNNEAARLWISGIEALNRKVGVRAVYDLSATPFFLQGSGYAEGTLFPWTVS
                     DFSLMDAIECGIVKLPRVPVADNLPEAEVPIYRDLWKHIGKEMPKKGAAKSGKLDPLS
                     IPAELQTALYALYGHYVKTYEEWRKVGIAVPPVFIVVCNNTSTSKLVYEWISGFERES
                     EDGEKVREHSGHLELFQNFDQYGNRLARPNTLLIDSEQLESGDALEPAFREMAGPEIE
                     QFRRERAQRSGAAGADDISDSELLREVMNTVGKPGRLGEQVRCVVSVSMLTEGWDTNT
                     VTHILGVRAFGTQLLCEQVVGRALRRQSYDLNEEGLFDVEYADILGIPFDFATKPVVT
                     PPKPPKPVTRVHAIKARERFTISFPRVTGYRKDLPQERITADFKADSKLLLTPELVGP
                     CTTLLEGIVGEGIEITPKVLEGIRQSTISFEIAKVLLYTRFREPGEQPPLHLFGQIKS
                     ITRRWIEEGYLECTGGTVPAMVTYREIAEKAAELIYLACQRGHSGTSRMKAILEPYNP
                     LGRTTHVSFNTSKSDLYATSGDRCHVNYVVCDSSWEAELARVVEAHPRVIAYVKNQGL
                     QFEVPYRDGAIPKKYLPDFIVQVDDGPITGARTQGSDDPLNIVIETKGYRGIDAQLKA
                     ETMASLWVPGVNNLGTHGRWAFAEFKDVFEIENAFAKLVDEAVGMVAA"
     gene            complement(6853..7125)
                     /locus_tag="PK264_19325"
     CDS             complement(6853..7125)
                     /locus_tag="PK264_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78061.1"
                     /translation="MAETDFKQLRARAVFSDAARVRLETTDEPIQAHDSVTGIDLHYR
                     FETHAGGVLRLTGGPIGEGTLAVGCEPESLNLIATILLDGLKVQIR"
BASE COUNT         1198 a         2182 c         2330 g         1461 t
ORIGIN      
        1 gacaatggtc tcgtcgccct gatcgagtat gtcgatgtcc ggctcgccga agatcacgaa
       61 caggttgccc ttgcccgtgt tcttcagctc gtcggccatg tgcaggtcgg gattcatctt
      121 ggccttgagg atcggcaggg ggccgagccg cgtcagctcc gaggcgtgag catcgaagtt
      181 gaaggagcag gcgatcaaaa catcgaagcg ggcttccgtc gcctcgcggg cggcggcgac
      241 caattcgacg cgggataggg tgccgaattc gggcccgagg aagatggcgg cgcgcctctg
      301 ggtctcgcct tcctggaagt gtccctcggc accgatgtat tcgccgggcc agcctgtcag
      361 ggacgagaac ttgatggtat cgcgcttctc cgcctggtgg acgccggcgg tgcgtagatg
      421 ctccagcacc atgtcagcga agtctgtcgg cggtgccaga aacttggaac gtcgaagctt
      481 gccttccgag gccttcagct cgtcggcgag catgtcctcg tcggccggca cgacgcggtg
      541 cggcgacagg ctttcgactg tgaaggggcc ggcgacgcgg acgcgggact tgtcttcata
      601 gggccggtcg tagagcatct cgacgtcggc ggccttggcg atcgaggcgt cgatcttcct
      661 ctgccgctcg atgcgcgctt cccaccattc aacatgcaac ttcttggccg cgtcggacca
      721 actcgggtca acctcacgcg ggatttccca ctcctcccag gacttggcca tcgctttgtt
      781 gagttgagcg cgcaggggtt cgagcgtagt ctgtgccgtc tcccagataa cgtcgatctc
      841 ggcattcctt gcgatcgagc tgagctggat gtgcggagcc cgctcacaga cgaagccctg
      901 tcgaatatcg ccgtgcgtcg gagtgtctgg ttggattctg cccgatatct cttgttcttt
      961 ggtgcggcct tctctgctgt ctgaaagtac atagtaaggg aaccgagaac tcatgatgcg
     1021 agtccgcgct agggcgagag caactcgaga ggagtcgatc gtggtccacc gccgacccca
     1081 ctgctcagag acataagctg tcgttcccga tccacaggtc ggatcgagga ccaagtctcc
     1141 agggtccgtc gtcatcaaga cgcaacgctc aacggctttt gtgggggtct gaacgacata
     1201 aatcttagga tcggcacgac tttggatgcc accaacgtct tcccaaaaat tgttcagctc
     1261 gacagcagaa aagtcatcga gaaaccggac gtatgctaga ctgttgccgg tcaattcaac
     1321 tctcgatgcg cgcaacaatc ggctcatccc ttgttgattt gttttccatc gcaccttgtc
     1381 agaggggcga attgttcgcc catcgatttc gacgggaaac catgatgcgg caccctcacc
     1441 cttctcgcga ccaacactct gagatgtgag attgtcctgt cggtacactc ggccatcaac
     1501 tgtctcaggt gagtttcgct cctcagcagt tattggacgt cgctgcaggt cacctagttg
     1561 caatcgattg tacttgtctg ctccttctcc ccccacttct ttaagtttgt agattgcccg
     1621 atatttcatt cgcccgatgt cacgggcaaa ccaaaggatg tactcctggg aattcgaaag
     1681 aaaaacagag gtggcgcctg ccgtcttgta gacggaaatc tggctgacga agttttcagt
     1741 gccgagcacc tcatccatca acgcccgaac acgatgaaga ttctcatcgc cgatctgcac
     1801 gaagatgctc ccgctatcag ccagcaggtc gcgcgcaacg gtcagccgat cgcgcagata
     1861 ggtcaggtat gaatggatgc cgtccttcca tgtgtcgcgg aaggccttca cctgctcggg
     1921 ttcacggctg atgtctgtct gcttgccatc cttcacgtcg cggctgagcg tcgagacctg
     1981 ccagttcgag ttgaacttga tgccgtaagg cggatcaaaa tagatgcact gcacctgccc
     2041 gcgcagatgc tcgcgctcgg cgaggctcgc catgacctgc aggctgtcgc cgaggatcat
     2101 gcggttcgac cagtgctggc tgtgctggta gaactcggtg cgtgcttccg ggtcggtcag
     2161 cccattgaag tcggcgaaca ggtccggcat gtcgtcggtg tcgggcctgg ccttcttcga
     2221 ctgccgcacg agatcgtcga ttatcgcctt gggatgcacc ttctcctgga tgtagagcgg
     2281 cggcgcgttg acgacgaggt cggaccagtc ctggctgtcc ttgccgcgcc acacgagctg
     2341 ggcgtcgccg atctcgatct cgcctttctc ctgaagctgg cgaacctgct ccttcgacag
     2401 gcggatacgt gcaccgttcc agatgatctg cggatcgatg tccttgtcgc gggcgcgctc
     2461 ggtgccggcc ttcagcggat agacgcgcga ataccggatc ggtccgacgg gcgccatttc
     2521 ctcctgccgt tgcgccaggc tctggtgctc ggctgtgggg atgtttttcc gccgcgcctt
     2581 ctcgtgcgtc agggcctcga ccctcttttc cttggccgcc ttcgcggttg cctttcttgc
     2641 cattacgcgg tgccccctag cacgttcttg attgcggcct cgtagcggtc agcctccttg
     2701 tggagccagt ccgcgatgcg atcccagttg gcgttgtcgg tcgcatcgag ccgcagttgt
     2761 ttgatgaaga gatagcgggg gttgcccgcg ttgatggacg catcgagcag gctttcgagc
     2821 ttctgctcgt agggatcgag aagttccgcc gcggcctcgt cgtcgccgcc ccgtggacag
     2881 cggatgaaga tgccggggcc acctttggcg atgtactgca ccaccgcgag cttcagctcc
     2941 ggtaggtcgc gccaccggga gctggcggca tagggctttg ccggatcgcc ttccgaaggg
     3001 tggcggctca acatatgagc ccagaactcg cgccggaagt tgcccaggtc ggtggtgggc
     3061 gccttgcgtt cgcgagccgt ctcctggacc ttgcggtccc agtggttggg gcgctcgatg
     3121 acgtcgaaga gcggcaccat cggcgacgtg ccgatgcgca ccaccttgat ctgaaccgcg
     3181 aagaaggcga acggctcctc ggtatgttcg ttgagccaac ggatggcgga gagatgcggt
     3241 tcgcggaagc tcggcgcgat ccagacgatg gtcgaggcgc cgaggccggc caggtaggtc
     3301 atgatctggc cgaggtggcc gtgatcactc ggcttgaact ggttctcgat caggacgacg
     3361 ctctcgtcct gcggattgcg tgcgatgatg tccgccgcaa agctctcgac cgccacttcc
     3421 gtatctgtca gctccaacgg aatgccgatc gcgtccgaca acagggcgag attctcagcc
     3481 agccacggcg tgaaggcgtg ggcctcgtgc gcccaggcct cggtgagtgg aacggtatcg
     3541 agcgtgccga acctgctcat gcggccacca ttccgacggc ctcatcgacc agcttggcga
     3601 aggcattctc gatctcgaac acgtccttga actcggcgaa ggcccaccgt ccgtgggtgc
     3661 cgagattgtt gacgccgggc acccagagac ttgccatggt ctccgccttg agctgggcgt
     3721 cgatgccgcg atagcccttg gtctcgatga cgatgttgag cgggtcatcg gagccctgcg
     3781 tgcgcgcgcc cgtgatgggc ccgtcatcga cctggacgat gaaatccggc aggtacttct
     3841 tcgggatggc gccgtcgcga tacggcacct cgaactggag tccctggttc ttgacatagg
     3901 cgatgacgcg cggatgcgcc tcgacgacgc gcgcgagctc cgcctcccag ctactgtcgc
     3961 agacgacgta attgacgtgg caccggtcac ctgaggtcgc gtagagatcg gacttgctgg
     4021 tgttgaagct gacatgggtg gttcgcccga gcggattgta cggctccagg atcgccttca
     4081 tgcggctcgt gccggagtgg ccgcgctgac aggcgaggta gatcagctcg gcggccttct
     4141 cggcaatctc gcggtaggtc accatggctg gaacggtgcc gccggtgcat tcgagatagc
     4201 cctcctcgat ccaccggcgc gtaatgctct tgatctggcc gaagagatgc aggggcggtt
     4261 gctcgccggg ctccctgaag cgcgtgtaga gaagcacctt ggcgatctcg aacgagatcg
     4321 tgctctggcg gataccttcg agcaccttgg gcgtaatctc gatcccctcg cccacgatcc
     4381 cttcgagcaa ggtcgtacac gggccgacca gctcgggcgt caggagcagt ttggaatccg
     4441 ccttgaagtc ggcggtgatc cgctcctgcg gcaggtcctt gcggtagccg gtgacgcgcg
     4501 gaaacgagat ggtgaagcgc tcgcgcgcct tgatggcgtg gacgcgggta acgggcttcg
     4561 gcggtttcgg cggcgtcacc acgggcttgg tggcaaagtc gaagggaatt ccaaggatgt
     4621 cggcgtactc gacgtcgaac agcccctcct cgttgaggtc gtaagactgc cggcgcaagg
     4681 cgcggccgac cacctgctcg cagagaagct gggtcccgaa ggcgcgcaca cccagaatgt
     4741 gcgtgacggt gttggtgtcc cagccttcgg tcagcatgga gacagagacg acgcaccgga
     4801 cctgctcgcc gagccgtccg ggcttgccga cggtgttcat gacctcgcgc aacagctcgc
     4861 tgtcggagat gtcgtcagcg ccggcggcgc ccgagcgctg cgcgcgctcg cggcggaact
     4921 gctcgatctc cgggccggcc atctcgcgga acgcgggctc cagggcgtcg ccggattcca
     4981 gttgctcgct gtcgatcagg agcgtgttcg ggcgggccag ccggttgccg tactggtcga
     5041 agttctgaaa cagctcgaga tggccggagt gttcgcgcac cttctcgcca tcctcgctct
     5101 cgcgctcgaa cccggaaatc cactcgtaga cgagcttcga ggtcgacgtg ttgttgcaga
     5161 cgacgatgaa caccggcggc acggcgatgc cgaccttgcg ccattcctcg taggtcttta
     5221 cgtagtgccc gtagagagcg tacagcgcgg tctgaagctc ggcggggatg gagagcggat
     5281 cgagcttgcc agacttggcg gcgcccttct tcggcatctc tttgccgatg tgcttccaca
     5341 gatcgcggta gatcgggacc tcggcctcag ggaggttgtc ggcgacggga acgcgcggca
     5401 acttgacgat gccgcattcg atggcgtcca tcagcgagaa atcgctgacc gtccagggaa
     5461 acagcgtccc ttccgcgtag cccgatccct gcaggaagaa cggcgtggcg gagaggtcgt
     5521 agacggcgcg cacgccgacc ttgcgattga gcgcttcgat gcctgaaatc cagagcctcg
     5581 cggcctcgtt gttcttcttg gcctcgtcct tgtcctcgcc cttgaactcc gcctcgtcgt
     5641 cggagcccgg tttctcacgg tagcagtggt gggcctcatc gttgatgacg acgacgttct
     5701 tcatggcgag aagctcgccg caggccctct ggagcatctg gccttcggtc tcgatggtga
     5761 ccacgggctc gccgcggccc tgcaggagcg cccggccgac cttcgagacg tccatggtct
     5821 cgcggcgctt gaaggcgtga tagttggcga tgacgatctt ggcccggtcg atgtcgggga
     5881 gcatgtcggg aggaagaagc tcgcgggtac ggtagtagct gtcggcatcc tggggcagga
     5941 gcacgcgcag gcggtcgcgg atggtgatgc cgggcgcgat cagcaggaag ccacgggtga
     6001 acagcgtgct cgtcggcgag cggaccgcat tgaccgtctg ccaggcaatc agcatggcca
     6061 tgacggtcgt cttgccgctt cccgtcgcca tcttgagggc gagccgcatc agctcggggt
     6121 tggcgtcctt gttggcgccc tcgacgtggg tccagagatg gcgataccgc ttctcgcgcc
     6181 gggcgacctc tgtcatccag atcaccgtct cgacggcttc cgtttgacag aagaagggcc
     6241 gaacgccctg gaagttgtga tggcgccagt gcttcaggag ctgggccgtt gccggtgtga
     6301 cgccccagtc ggccggattg gggatggtcc gccaggtctc gatgtgctgg cggatctcat
     6361 tgatgatcgg ggtcgggttg tattcctgct ccgaggtgga gaggcccgcc ggatcggaga
     6421 agccgaggct cgcctgggcg gcccctgccc gcttcagaag ctttcgcggc ttcgggaccg
     6481 gcgtgatcag ctcggagcgg cggcggccgt ccaccggcgg ctgatcgagc ggctggccat
     6541 ccttgtcgag cgcatgatgg cgcgtcggcg gctcgtacgg cgaattgagg atcggcgcct
     6601 cgaagaagga tgcagtcata ggccctccgc acagcgtagc cggccatgcc cagaaattgg
     6661 ttcgcggtcg aaacgtcggc tcatgcggcg tggcttcccc caaagatttc attagcgtag
     6721 cagaggggcg ggaatcgacc cagaggcgag cacaatcggt ccccagaagc ttagcggaac
     6781 cgtgggcgga atggcctcat cggccggcac cgcggcaccg cgcctgtgga ccactcttgc
     6841 tccagggcgg gatcagcgga tttgcacctt cagcccatcc agcaggatcg tcgcgatcag
     6901 gttgaggctc tccggctcgc atccgacggc tagggtcccc tccccgatcg ggccgccggt
     6961 caggcgcagg acgccgccgg cgtgtgtttc gaagcggtag tgcagatcga tgccggtgac
     7021 ggaatcatgc gcctggatcg gctcgtcggt cgtctccaga cgaacgcgcg cggcatccga
     7081 gaataccgcg cgcgcccgta gttgcttgaa atcggtctca gccattggcc ggagacttcg
     7141 ggcccttgct cccctgcccg gctgtctcga g
//
LOCUS       DAORNH010000239         7061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7890_length_7061_cov_2.586212, whole genome shotgun sequence.
ACCESSION   DAORNH010000239 DAORNH010000000
VERSION     DAORNH010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7890_length_7061_cov_2.586212"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..350
                     /locus_tag="PK264_19330"
     CDS             87..350
                     /locus_tag="PK264_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78062.1"
                     /translation="MHLMKDAQRKHTRLVRSAYKTFARFYGCDASDWDQVYNILYSLH
                     YFCHARDIDFRACLEDTEESFLVNKQTFEAFDADAGDALRDWR"
     assembly_gap    1064..1104
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1105..1406
                     /locus_tag="PK264_19335"
     CDS             <1105..1406
                     /locus_tag="PK264_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78063.1"
                     /translation="TDDRAAELVLSLTQKEHAALLARAGAAGVDVRRYVIDRALEDAP
                     RIDPATQFEAVDSLMRIGTLLHRLVDISRSTGVVPEEVLGVIDRVERALEHRLTA"
     gene            1628..3172
                     /locus_tag="PK264_19340"
     CDS             1628..3172
                     /locus_tag="PK264_19340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HRD78064.1"
                     /translation="MAWLAANAMSVRQAAVAAAARAAGMSYADYVRETNPFRGNKGRK
                     PVYTLSIAWHPEKNRMPTRAQMIQAAEEVLGVLGFKDHQAVIVQHTDTKHPHLHLIVN
                     RVHPTTGLYAKTGNDWLKLSRWALEHEKKTGLILCWERFENWKKRDAARELKAERRKT
                     DPKAKGEWILARDVPRRDHDWFRSVAHLPAPEIRQARAARQEHEREQFRRIETSRNAT
                     INREIVRFYGPERRALDLEIRSLMGAEASRERRFAHPIALLLSPRDAFKAVVELITAR
                     AYARPRRIRALQKAERDLQAAIQQRKTVENRRHVQAWDRLLVRQDAERRRDDERIAAL
                     ARAARGKGTADRGRQVFNLRGNVETARYVSPRQPLVRLSDVHARVSRTAEAGRTAARN
                     ALAKVVRLLGGNATTEDLRKRTIGGRAPPVMPDGNRVSIVVPGNQIEKITPDRLPVSK
                     ADHRDDDRRQNDRRAPGRRVPVTEAERVAFEARRDAALERIEADNEERRRRKRVRPRG
                     RSRKME"
     gene            complement(3176..3871)
                     /locus_tag="PK264_19345"
     CDS             complement(3176..3871)
                     /locus_tag="PK264_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78065.1"
                     /translation="MAAKSLLRQRNSLSYPGILHVSPPLEAGRRLLFCYPVGVKYCIY
                     ALVDPRTDELFYIGQTSHLSRRRTEHLDGTDQLSGVRVAQIKVAGFLPYVIVLERRTD
                     MASALSAEVFWIELALSRGMTLLNAQGVGGHVARRRERRSLHADLDAMTSAKRDAGTL
                     ATIANGRSLRMGETWSDQELRRLAGMVRAGMSREAMADALGRSPGDICRRLDLESRGT
                     SKSRRRRGRRPRQ"
     gene            3870..5030
                     /locus_tag="PK264_19350"
     CDS             3870..5030
                     /locus_tag="PK264_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78066.1"
                     /translation="MLIAVESHGPDSARTAPASLPERSQGLQYDLFTQFFGKPENLSN
                     TIEFWDVLPKYSFSARMQALSRDAAGRLPVYQRTFEYRPAPKAGNPTLTCTLSLQPAS
                     IETDGRRIDCYPSADEELVEEVLKKIFTDQSCGFHDSRRGESWVRFTLHMIRSELARR
                     GRTRSIAEIKRSLEILSKTVLDVAIEGPGARRSVYTNPILNDLIRVSRAEWLDDPTAT
                     WAARLPGLVSASVDALSYRQFNYAVLMSLKAPLARWLHKRLSHEYTNAHLTSPYRILF
                     STITRDSGLLHNCRASANLKAVEQALSELAEHRVLLSHTAERRHRGSAIDDVLYTLTP
                     HMDFVGEIKAANARANEARARLQGAGLLALPTETAVEAGPRRLSVPRRLARS"
     gene            complement(5935..>7061)
                     /locus_tag="PK264_19355"
     CDS             complement(5935..>7061)
                     /locus_tag="PK264_19355"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRD78067.1"
                     /translation="AGLADLAIDLAGRSASFKASLPEGIAAALAALVRAMNCYYSNLI
                     EGHNTHPIDIERALKGDYSTDKGKRDLQLEARAHMTVQAWIDGGGIAGHPTDVGVLQD
                     IHRRFCELLPPDLLFVEDPVSGERVAVVPGALRHRDVQVGRHRAVSPGAVPRFLARTA
                     EVYGRLGRAEAILAVAAAHHRLLWVHPFLDGNGRVARLVSHAMFLKSVDSGALWSIAR
                     GFARNEAAYKGHLAACDLGRRNDLDGRGNLSEEALADFTRFVLTTAIDQVEFMRGLIE
                     PDRLRARILLWAEEEARAGRLVPRAGQVIEAILYRGELPRGEVPAILGLGDRQARRVT
                     QQVLSDGILTAAGPRAPLRLAFPATLAGRWMPGLFPERAD"
BASE COUNT         1225 a         2491 c         2236 g         1068 t
ORIGIN      
        1 tcgattgacc ctcgcgccat cgcaccgcga tgcccgccgt ccatgcgcag gaatggtcct
       61 gccaccgaaa ccacaaggag accccgatgc acctcatgaa agacgcccaa cgcaagcaca
      121 cccgcctcgt ccgatccgcc tacaagacct tcgccaggtt ctacggttgc gacgccagcg
      181 actgggatca ggtctacaac atcctctaca gcctgcatta cttctgtcac gccagggaca
      241 tcgacttccg ggcatgcctc gaagacaccg aggagagttt cctcgtgaac aagcaaacct
      301 tcgaggcctt cgacgccgat gccggcgacg ccctccggga ctggcggtag ccagtccctt
      361 ccctgacggt cggccacggt gccgacccac ttcgtggcca cgctcgtcac caagccatgg
      421 cgcgcgggcg atggcgcgcc agatccctcg cacctgtcga ccgtctcgca ctgtatccgc
      481 tgacccgtcc gtggcgaacc cgggcacggc gccaggcgga gcgtcaaccg ctccgggcct
      541 gcgccgtgcg cccctcgtcc cgcttcgcgc tgacgtgcgg cgctggacga catatgccgc
      601 cagcgctacg caagatgtcc cggcattatg caattgccga tttcattgcg caggaattgt
      661 tattatcgcg attatgtcct gccggcgata attgtcgttt tttccggcgg ttcattctgc
      721 tatcggtgcc tctgtcagcg tgaatgtaac gagatatgtt tttcgggtcc gaaaaacaga
      781 aaatgagggg ggccttaggg ccgccgcccc tctttgcgcc ggatgagccg gcgcaaattc
      841 accccagccc acggcgcgtc gaaagccacg ccggagacgg gagcaccgct gacacttgtg
      901 tgaaacgtgt accactgtgt gtgactgagc catggcaaga ccccgcaagc gcgacgccga
      961 ccggcgcgac cgcaagatca tcgtctgggt gacggctgcc gagcaggccc gctatctcgt
     1021 caactcggcg cggacgggtc tgaccggacc cgactacatc cggnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnccaccg atgatcgtgc cgccgagctg gtgctatccc
     1141 tgacgcagaa ggagcatgcc gccctgcttg cccgcgccgg tgccgccggc gtcgatgtcc
     1201 gacgttatgt catcgaccgc gccctcgagg acgcgcccag aatcgatccg gcgacgcagt
     1261 tcgaagccgt cgacagtctg atgcgcatcg gcaccctcct gcatcgtctg gtcgacatct
     1321 ctcgttcgac cggcgtcgtg cccgaggagg tcctcggcgt catcgaccgg gtcgagcgcg
     1381 ctctggagca ccggttgacg gcatgatccc gcgcgtcgcc aagctcggga caggcttcgt
     1441 cggcgccggg aaatactacc tgcacgacaa gcggccggac ccggccgagc gcgagcggcc
     1501 gtctgccggc gactacatgc ttcacgacaa ggccgtcgcc ggcgtactgg cgcagaccaa
     1561 ccatcgcgtc ggattgacgg acacccgcaa cctgccgacc cgcgatcccg agaaggcgct
     1621 gaagtgcatg gcctggcttg ccgccaacgc catgtccgtg cggcaggcgg ccgtggccgc
     1681 cgccgcgcgc gccgccggca tgagctatgc cgactatgtc cgggagacga accccttccg
     1741 cggcaacaag gggcgcaagc ccgtctacac gctttccatc gcgtggcacc cggagaagaa
     1801 ccggatgccg acccgggccc agatgatcca ggccgccgaa gaggtccttg gcgtgctcgg
     1861 cttcaaggac catcaggcgg tcatcgtcca gcacaccgat accaagcacc cgcatttgca
     1921 cctgatcgtc aatcgcgtgc atccgacgac ggggctttac gcgaagaccg gcaacgactg
     1981 gttgaagctc tcgcgatggg ccctggagca cgagaagaaa accggcctca tcctgtgctg
     2041 ggagcggttc gagaactgga agaagcgcga cgcagcgcgg gagctgaagg ccgagcgccg
     2101 caagaccgat ccgaaagcca aaggcgaatg gatactcgcc cgtgacgtgc cgcgccgcga
     2161 tcacgactgg ttccgctccg tcgcgcatct gccggcgccc gaaatccggc aggcccgcgc
     2221 cgcccgccag gagcacgagc gcgagcagtt ccgccgcatc gagacctcgc gcaatgccac
     2281 catcaaccgc gagatcgtcc gcttctacgg accggagcgt cgcgcgctcg atctcgaaat
     2341 ccgaagcctg atgggcgccg aggcgagtcg cgagcggcgc ttcgcgcacc cgattgccct
     2401 cctgctctca ccgcgcgacg ccttcaaggc cgtggtcgag ctcatcaccg cccgcgccta
     2461 cgcccgaccc cgtcgcatcc gggccctgca gaaggccgag cgcgatctgc aagccgccat
     2521 ccagcagcgc aagacggtgg agaaccgccg gcacgtccag gcctgggacc gcttgctcgt
     2581 gcgccaggat gccgagcgtc gtcgcgacga cgagcgcatc gccgccctcg cccgcgccgc
     2641 acgcggcaag ggcaccgccg atcgcggccg gcaggtgttc aacctgcgcg gcaatgtcga
     2701 gacggcccgc tacgtgtcgc ctcggcaacc gctcgtgcgt ttgtcggacg tccatgcccg
     2761 cgtgtcccgg acggccgagg cgggacgcac ggccgcccgc aacgcattgg ccaaggtggt
     2821 ccgtctgctc ggcggcaacg ccaccaccga ggatctgcgc aagagaacga tcggaggccg
     2881 agcgccgccg gtgatgcccg atgggaatcg ggtttcgatc gtggtgccgg gcaaccagat
     2941 cgaaaagatc acgccggatc ggctgccggt gagcaaggcc gatcaccgcg acgacgaccg
     3001 acgacagaac gaccggcgag cgccaggccg gcgggtgcca gtgaccgagg ccgaaagggt
     3061 cgcgttcgag gcccggcgcg atgcggccct ggagcgaatc gaagcagaca atgaggaacg
     3121 ccgacggcgc aagcgcgtca ggccgcgagg caggagccgc aagatggagt gacactcact
     3181 ggcggggacg ccgcccgcga cggcgcctcg atttgctggt gccgcgactt tccagatcga
     3241 ggcggcggca gatatcacca ggtgagcgac cgagcgcatc ggccatggct tcccggctca
     3301 tgcccgcccg caccatgccg gcgagacggc gcaactcctg atccgaccat gtctcgccca
     3361 tccgcagact gcggccgttg gcgatcgtcg caagcgtgcc agcgtcgcgc ttagccgacg
     3421 tcatggcgtc gagatcggca tggaggcttc tgcgctcgcg tcggcgggcg acgtggccgc
     3481 cgacgccctg tgcgttgagc aaggtcatgc cccgagagag tgccagctcg atccagaaca
     3541 cttccgccga caaggccgac gccatgtcgg tccggcgttc gagcacgatc acgtagggaa
     3601 ggaagccggc taccttgatc tgcgccacgc gcacgcccga cagctgatcc gtgccatcga
     3661 gatgctccgt ccggcgccgg gacagatgcg aggtctggcc aatgtagaac aactcgtcgg
     3721 tgcgcgggtc gacgagcgcg tagatgcagt acttcactcc gacaggatag cagaacagga
     3781 gccggcgacc agcttcaagt ggtggactta catgtagaat tccgggatag gagagtgaat
     3841 tccgttgacg gagcaacgat ttcgcggcca tgctgatcgc cgtggagtcg catggccctg
     3901 atagcgcccg gacggcgccg gcctccctgc ccgagcggtc gcagggcctc cagtacgacc
     3961 tcttcaccca gttcttcggc aagcctgaga acctctccaa caccatcgag ttctgggacg
     4021 tcctgccgaa gtacagcttc tcggcccgca tgcaggcgtt gagccgcgat gccgccggcc
     4081 gcctgcccgt ctatcagcgg acgttcgaat accgcccggc gcccaaggcc ggcaacccga
     4141 cgctgacctg cacgctgagc ctccaacccg ccagcatcga gaccgatggc cggcgcatcg
     4201 actgttaccc ctccgccgac gaggaactgg tcgaggaggt cctgaagaag atcttcaccg
     4261 accagtcctg cggcttccac gacagccgcc gtggcgagag ctgggtacgc ttcactttgc
     4321 atatgatccg atccgagctg gcgcgccgcg gccgcacccg ctcaatcgcc gagatcaagc
     4381 gctcgctgga gatcctctcc aagaccgtgc tcgacgtcgc catcgagggc cccggcgccc
     4441 ggcggagcgt ctacaccaac ccgatcctca acgacctgat ccgcgtctcc cgcgccgaat
     4501 ggctcgacga tccgaccgca acctgggcgg cccgcctgcc gggcctagtc agcgcctcgg
     4561 tcgacgcgct ctcctaccgg cagttcaatt atgccgtgct gatgagcctc aaggccccgc
     4621 tcgcccgctg gctccacaag cggctctcgc acgagtacac caacgcgcac ctgaccagcc
     4681 cttaccgcat cctgttctcg accatcacgc gggattcagg gctcctgcac aattgccggg
     4741 ccagcgccaa cctgaaggcc gtcgagcagg cgctgagcga gctggccgag caccgcgtgc
     4801 tcctctcgca caccgccgag cgccgccacc gcggcagtgc catcgacgac gtgctctaca
     4861 cgctcacgcc gcacatggac ttcgtcggcg agatcaaggc cgccaatgcc cgcgccaatg
     4921 aggcgcgcgc ccgactccaa ggcgccgggc ttctggcgct ccctactgag accgcggtcg
     4981 aggccgggcc gcgtcggctg tcggtcccgc gccgtctagc ccgatcgtga tctgcacgat
     5041 gtgccttcca attgcgctcg ccatgcccgt caggtggaag gcggaacatg ccagtgccgg
     5101 gaggcggagc gtgcaatcgg tggcaggtgg agcgtgccgg gcgggttttc gactgctcgc
     5161 gcgaaggatc acgaagcgtc caggcgggag gcagagcgtg cagaggtgcc ggatcgccgg
     5221 caggtggagc gtgcaggtcg cggccggccg gcgctgtgcg ttcagcgaat cgggaggcga
     5281 agcgtgcaat acgccccgac ttgggaggcg gagcctgcag ggccgtcggc gccagcccgc
     5341 ggaatccgcc gaccgactcg gaaacccgca cggcgtatcc cttccctatt catatccttc
     5401 ttgaaccttg ggcgacgatc gactgttggg gtaccagagc ctcgcttcgc tcggataaag
     5461 gacgggaccg caacaggcgg tcccgacttg gtgtcccctc accctccgct cgcgcttcgc
     5521 gctcactgcg ccgcacctgt tggggtttcg tggagcgtga tcgtacagga ctgaaaccct
     5581 ggagatacgt cgaatcttca gcctttgctc ggcgagcgac caagcggccc cctgcggcgg
     5641 acgcccggcg atctcccggc aaacggcacg cccggcccga tcctgatcgg ctgatgcggt
     5701 tgcgatgaag ggcccgctga ccccctgccc cgtgactgtc cgcccgcgtc cggacaacgt
     5761 cgcggacagc gcgaccatca tggccgcagt gatcggcact ttcgggaaag gtgagaaatt
     5821 gggcgccaca aattggcgct gatccacttt tgagcgccag attcggtctg ttctggcgct
     5881 caaacggctc attgtggcgc tctgggcact tgcgtccaag aaaccgtgat gccctcagtc
     5941 ggcgcgttct ggaaacaggc ccggcatcca gcggccggcc agagtcgccg gaaatgccag
     6001 ccgcaacggc gcccgcgggc cggccgcggt caggatgccg tcgctcaaga cctgttgcgt
     6061 gacccgccgc gcctgtcggt ccccgagccc gaggatcgcc ggcacctcgc cgcgcggcaa
     6121 ctcgccccga tagaggatcg cctcgatcac ctgacctgcc cgcggcacca gacgaccggc
     6181 ccgagcctcc tcctcggccc agagcaggat gcgggcccgg agccggtccg gctcgatcag
     6241 gccgcgcatg aactctacct ggtcgatggc cgtcgtcagc acgaaacgcg tgaagtccgc
     6301 gagcgcttcc tcgctcaggt tgccgcgccc gtcgagatcg ttgcgccggc ccaagtcgca
     6361 ggccgcgaga tgacccttgt acgccgcttc gttgcgcgca aagccgcgcg cgatcgacca
     6421 cagcgcgccg ctgtcgacac tcttcaggaa catggcgtgg ctgaccagcc gcgccacacg
     6481 gccgttgccg tcgaggaacg ggtgcaccca gagcagacgg tgatgcgccg ccgccaccgc
     6541 gaggatcgcc tccgcccgcc cgagccggcc atagacctcg gccgtgcgcg ccagaaaccg
     6601 cggcacggcg ccggggctta ctgcccggtg ccggccgacc tggacgtcgc gatgtcgaag
     6661 cgcacccggc accaccgcca cacgctcgcc ggagaccggg tcctcgacga acagcaggtc
     6721 cggcggcagg agctcgcaga agcgccggtg gatgtcctgg agcacgccca cgtcggtcgg
     6781 atggccggcg atgccgccgc catcgatcca ggcctgcacc gtcatgtgcg cccgggcctc
     6841 gagctggagg tcgcgcttgc ccttgtcggt gctgtagtca cccttcagtg cccgctcgat
     6901 gtcgatcgga tgcgtgttgt ggccctcgat caggttcgag taatagcagt tcatcgcccg
     6961 caccagcgcc gccagcgccg ccgcgatccc ttctggcagg ctcgccttga acgaggccga
     7021 acggccggca agatcgatgg cgagatcggc gagcccggcc c
//
LOCUS       DAORNH010000240         7060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7893_length_7060_cov_2.316916, whole genome shotgun sequence.
ACCESSION   DAORNH010000240 DAORNH010000000
VERSION     DAORNH010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7893_length_7060_cov_2.316916"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK264_19360"
     CDS             <1..332
                     /locus_tag="PK264_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRD78068.1"
                     /translation="ATRLETAVRVVLPAAFSGLAAAFILAISRAVGETMVVAIAAGQQ
                     PNLTFDPREPAATITAFIVQVALGDLPHGSIGYQSIFAVGLVLFLMTLTFNIIAFWLR
                     RRYREAY"
     gene            332..1294
                     /gene="pstA"
                     /locus_tag="PK264_19365"
     CDS             332..1294
                     /gene="pstA"
                     /locus_tag="PK264_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRD78069.1"
                     /translation="MTAASTGPGADAIRALDQAEARRTRNARRRTRERVFRAVGLTAT
                     LVGLVALAALLIDLLVTGAPRLTWDFVTSFPSRRASQAGILSAWVGSLLVILVTASAA
                     VPIGIAAGIYLEEYAPRNWITDIIEVNVSNLAGVPSIIYGLLALGLFVYGLGTGRSIF
                     TAGLTLALLILPIIIVATRESIRAVPSSLREAALGLGATDWQVSRDHVLPAALPGVLT
                     GVIIGISRALGETAPLITIGALTFIAFLPAAPIQSSPPFVNMQWLWDGFTVLPIQAFN
                     WVSRPGNDFEANAAAAGVVLLVITLGLNAVAIWLRARLRRKLKW"
     gene            1318..>2138
                     /gene="pstB"
                     /locus_tag="PK264_19370"
     CDS             1318..>2138
                     /gene="pstB"
                     /locus_tag="PK264_19370"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRD78070.1"
                     /translation="MHGTFSVEADAARRSPSSDARTAAEVKLTSRGVNVFYGSKHALV
                     DVGVDIPNRMVTAFIGPSGCGKSTFLRCINRMNDTIEGCRVTGELKIDDRDIYARDVD
                     PVELRSRVGMVFQKPNPFPKSIYDNIAYGPRIHGLAKSRADLDAIVERSLAKAGLWDE
                     VKDRLKDAATGLSGGQQQRLCIARAIAVEPEIILMDEPCSALDPIATARIEALIEELR
                     QSYCIVIVTHSFAQAARVSQRTAFFHLGLLVEEGPTEQIFSTPRDQRTQDYITGR"
     assembly_gap    2139..2378
                     /estimated_length=240
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2379..2881
                     /gene="phoU"
                     /locus_tag="PK264_19375"
     CDS             <2379..2881
                     /gene="phoU"
                     /locus_tag="PK264_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRD78071.1"
                     /translation="KRQPMASDLRHIMTVLKIGGDLERIGDLAKNIAKRALATSSEPQ
                     PRSLLVGMTHMADQAHRQLKDVLDAFSVRDQAKALAVWRGDASLDALYNSVFRELLTY
                     MMEDPRNIGLCTHLLFGAKNLERIGDHTTNIAENIHYLLTGTQLTDARPKGDDTSSVL
                     FSKPPG"
     gene            2885..3577
                     /gene="phoB"
                     /locus_tag="PK264_19380"
     CDS             2885..3577
                     /gene="phoB"
                     /locus_tag="PK264_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HRD78072.1"
                     /translation="MTIRILVVEDDPAISTLLAYNLEAAGYAVTTVTGGEDAELHLAS
                     STFDLVILDWMLPEMTGIEFCARLRKSGGHPQLPVLMLTARGEEADRVRGLSTGADDY
                     VVKPFSVPELMARITALLRRASPERMNDVIGHGDITLDRTAHRVTRGVRAVELGPTEF
                     RLLVALIESRGRVLTRAQLIDRVWGNASDIDDRTVDVHIGRLRKALIRGKEADPIRTV
                     RGTGYALTEPGD"
     gene            3856..3973
                     /locus_tag="PK264_19385"
                     /pseudo
     CDS             3856..3973
                     /locus_tag="PK264_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317742.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
     assembly_gap    3974..4032
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4033..5455
                     /locus_tag="PK264_19390"
     CDS             <4033..5455
                     /locus_tag="PK264_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD78073.1"
                     /translation="RHFPLATMKPGDVYITNDPWMGTGHTNDFVLTTPCFHKGRLVAL
                     FSCTSHLMDIGGLGFGPDATDVFMEGLYIPMLRLAEGGKLNDTLMAMIRANTRLPVDT
                     EGDVYSLANCNDVGCKRLVDMMTEFDLDDLDTLADHICRVSEAGVLAEVAKLPHGTWK
                     NAMRVDGYEEPVDLVATLTVGKGGIHVDYTGTSAESRRGINVPMSYTTAYTVFGLGCV
                     VASRIPNNAGSLGPLTVSAPAGSILNAQKPKPVLLRHIIGQMLPDVVFGCLRQAIPER
                     VPAEGTSCLWNLNVRGQTPDATRGNYGFMMTVTSNGGTGARPGKDGMSATAYPSGVKG
                     TPVEIAESITPLIFWKKELRPDSGGAGTSRGGLGQVIEIESRIGMPFDLLAGFDRIDN
                     PPRGRDGGANGEAGSVSLKSGKPLKGKGMQQIPPGERLVIHTPGGAGIGSPATRPRDA
                     VATDLADGLVTEKTAKGIYAAPR"
     gene            5490..5575
                     /locus_tag="PK264_19395"
     tRNA            5490..5575
                     /locus_tag="PK264_19395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5524..5526,aa:Leu,seq:cag)
     gene            5840..>6662
                     /locus_tag="PK264_19400"
     CDS             5840..>6662
                     /locus_tag="PK264_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78074.1"
                     /translation="MKMRGVFASLLLAGCSTHPISADVTLDTYSIATHVECEAYRALS
                     SVLAGYLVKHDDPYLQQLGRDLFSGLQGKNGRHLNKLTVDELLRLSATDRTKIVPWDA
                     VRIGFGYTFKITEKNDNGGSAAVNVPIGIATLTVGVAAAANRARESLREFEIVMKLSE
                     LIKSQACDAPRARDGNAHTYPISGQIGLVESFGTFTALWLSSGENLKGPGRTIGTKLD
                     GFVDQITFTTTIGGGITPEVTLAAVKTGPRVTKGTLSHASSRTDAHKLILVIVPPS"
     assembly_gap    6663..6787
                     /estimated_length=125
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1152 a         2333 c         2013 g         1138 t
ORIGIN      
        1 gcgcgaccag gctcgagacg gccgttcgcg tcgtgctgcc ggccgcgttc tcaggcctcg
       61 ccgccgcctt catcctggcg atctcgcgcg cggtcggcga gacgatggtg gtcgccatcg
      121 ccgccggaca gcagccgaac ctcaccttcg acccgcgcga gccggccgcc accatcaccg
      181 cattcatcgt ccaggttgcg ctcggcgatc tgccgcatgg ctcgatcggc tatcaatcca
      241 tcttcgccgt cgggctcgtc ctctttctga tgacactcac cttcaacatc atcgcgttct
      301 ggctgcgccg gcgctaccgg gaggcctact gatgactgct gcatcgaccg gacctggtgc
      361 ggatgccatc cgcgcgctcg atcaggccga agcgcgccgg actcgcaacg cgaggcgccg
      421 gacgcgcgag cgcgtcttcc gcgcagtcgg cctcacggcg actctcgttg ggctcgtcgc
      481 gttggccgcc ttgctgatcg atctcctcgt caccggtgct ccgcgcctca cctgggattt
      541 cgtcacgagc tttccgtcgc gccgggcgtc gcaggccggc atcctctcgg cctgggtggg
      601 ctcgctcctc gtcatcctcg tcactgcgtc ggctgccgtc cccatcggga tcgccgccgg
      661 catctacctc gaggaatacg cgccgaggaa ctggatcacc gacatcatcg aggtcaacgt
      721 ctccaatctc gccggcgtgc cgtcgatcat ctatggtctg ctcgcgctcg ggctcttcgt
      781 ctatggcctc ggcacggggc gctccatctt caccgcagga ttgacgctcg cgttgctgat
      841 cctgccgatc atcatcgtgg cgacgcgcga gtcgatccgc gccgtgccgt cgagcctccg
      901 cgaagcagcc ttgggcctcg gcgcgaccga ctggcaagtc agccgcgacc atgtgctgcc
      961 cgcggcgctg ccgggcgtgc tgacgggcgt tatcatcggc atcagccgcg cgctaggaga
     1021 gaccgcgccc ctcatcacca tcggcgcgct gacgttcatt gcgttcctgc cggccgcgcc
     1081 gatccagtcc tcgccgccgt tcgtcaacat gcaatggctc tgggacggct tcacggtcct
     1141 gccgatccag gccttcaatt gggtctcgcg gcccggcaac gacttcgagg cgaacgccgc
     1201 cgcggccggc gtcgttctcc tcgtcatcac gctcggcttg aatgcggtcg cgatctggct
     1261 cagggcccgc ctgcgccgga agctcaaatg gtaggttggc cggaaggaca tgaagcaatg
     1321 cacggcacat tttcagtaga ggccgacgcg gcacggcgaa gccccagctc ggacgcacgc
     1381 acggcggccg aggtcaagct cacgtcgcgc ggcgtcaatg tattctacgg ctcgaaacac
     1441 gccctcgtcg atgtcggcgt cgatatcccg aacaggatgg tgacggcctt catcggtccg
     1501 tcaggctgcg gcaagtcgac cttcctgcgc tgcatcaatc gcatgaacga cacgatcgag
     1561 ggctgtcgcg tcaccgggga gctcaagatc gacgaccgtg acatctacgc ccgcgatgtc
     1621 gatcccgtcg agctccgctc acgcgtcggc atggtcttcc agaagcccaa tcccttcccg
     1681 aagtcgatct acgacaacat cgcctatggc ccgcgcatcc acggcctcgc caagtcgcgt
     1741 gccgacttgg atgcgatcgt cgagcggagc cttgctaagg ccggcctgtg ggacgaggtg
     1801 aaggaccgat tgaaggacgc ggcgaccggc ctctctggcg gccagcagca gcggctctgc
     1861 atcgcccgcg ccatcgccgt cgagcccgag atcatcctca tggacgagcc ctgctccgcg
     1921 ctcgacccga tcgctacggc ccgcatcgag gcgctgatcg aggagctgcg gcaaagctac
     1981 tgcatcgtga tcgtcacgca ttccttcgcg caggccgccc gcgtctcgca gcgcaccgcc
     2041 ttcttccact tgggcctcct cgtcgaggag gggccgaccg agcagatctt ctcgacaccg
     2101 cgcgaccagc gcacccagga ctacatcacc ggcaggttnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc aagcgccagc cgatggcgag
     2401 cgacctccgc catatcatga ccgtcctcaa gatcggtgga gacctcgagc gcattggtga
     2461 tctcgcaaag aacatcgcca agcgggcact tgcaaccagc tccgagcccc agccgcgatc
     2521 actgcttgtc ggcatgacgc atatggcgga ccaggcccat cgccagctca aggacgtgct
     2581 cgatgcgttc agcgtgcgcg accaggccaa ggcgctcgcg gtctggcgtg gcgatgccag
     2641 tctcgatgca ctctacaaca gcgtgttccg ggagctgctc acctacatga tggaggatcc
     2701 tcgcaacatc gggctgtgca cgcatctcct gtttggcgcc aagaacctcg agcggatcgg
     2761 cgaccacacg accaatattg ccgagaacat ccactacctg ctgacgggca cccagctgac
     2821 cgacgcccgc cccaaaggcg acgacacgag ctcggtgctg ttcagcaagc caccgggctg
     2881 agccatgacg atccgcattc tcgtcgtcga ggacgatcct gcaatctcca cgctcctcgc
     2941 ctacaatcta gaggctgccg gatacgcggt cacgaccgtc acgggcggcg aggatgccga
     3001 gctgcacctc gcctccagca cattcgatct ggtcatcctc gactggatgc tgccggagat
     3061 gacggggatc gagttctgcg cgcggctgcg caaaagtggc gggcacccgc aactgcccgt
     3121 gctgatgctc accgcccgcg gcgaggaggc agaccgcgtg cgaggccttt ccaccggcgc
     3181 cgacgactac gtcgtgaagc cgttctccgt tccggagctc atggcgcgca tcaccgcact
     3241 gctgcgccgc gcctcgccgg agcggatgaa cgacgtcatc ggccatggcg atatcactct
     3301 cgaccgcacg gcgcatcgcg tgacgcgtgg cgtccgcgcc gtcgagctcg gcccgaccga
     3361 gttccgcctg ctggtagcac tcatcgagag tcggggtcgc gtgctgacgc gcgcccagct
     3421 catcgaccgt gtctggggca acgcctccga catcgacgac cgcaccgtcg atgtgcacat
     3481 aggccgccta cggaaggccc tcattcgcgg caaggaggcc gatccgatcc gcaccgtccg
     3541 cggcaccggc tatgccctca ccgagcccgg cgactgagat tggccgggcg ccgatcccgc
     3601 atccctgtag cctcgcttcg cgagcgaagt cccgccccgc agcgccggtg gagatcctcc
     3661 ggaccgcccg ttattgaagc acgttggcag ggtcgccagg ggtgcgggcc gagcaggccc
     3721 gctcgctgag cgacgcgcct cgaccgccgc cccgcgcccg gataagttgc ttccagcaac
     3781 gcccgatcga agcaggagcc catgcgcgcg aaccggatcg acgaaatcga gctgcaggtg
     3841 atgtggaacc gcctgatcgc ggtcgtcgag gagcaggcgc agacgctgct gcgcaccgcc
     3901 ttctcgccga tcgtgcgaga gtgcggcgac ctttccgccg gcgtcttcga caccaagggg
     3961 cgcatgctcg cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnccgccact tcccgctcgc caccatgaag ccgggcgacg tctacatcac
     4081 caacgatccc tggatgggca ccggccacac caatgatttc gtgctgacca cgccctgctt
     4141 ccacaagggc cgcctcgtcg cgctcttctc gtgcacgagc catctcatgg acatcggcgg
     4201 cctcggcttc ggcccggacg cgaccgacgt cttcatggaa ggcctctata tcccgatgct
     4261 gcgcctcgcc gagggcggca agctcaacga cacgctgatg gcgatgatcc gcgccaacac
     4321 gcgcctcccc gtcgacacgg agggcgacgt ctactcgctc gccaactgca acgacgtcgg
     4381 ctgcaagcgc ctcgtcgaca tgatgaccga gttcgacctc gacgacctcg acacgctcgc
     4441 cgaccacatc tgccgcgtct cggaggcggg cgtcctcgcg gaggtcgcca agttgccgca
     4501 tggcacctgg aagaacgcca tgcgcgtcga cggctacgag gagccggtcg acctcgtcgc
     4561 gacgctcacc gtcggcaagg gcggcatcca cgtcgactac acggggacga gtgcggaatc
     4621 gcgccgcggc atcaacgtgc cgatgtccta cacgaccgcc tacaccgtgt tcggcctcgg
     4681 ctgcgtcgtc gcctcgcgca tcccgaataa cgcaggctcg ctcggccccc tcaccgtttc
     4741 ggcaccggcg ggctcgatcc tcaacgcgca gaagccgaag ccggtgctgc tccgccacat
     4801 catcggccag atgctgcccg acgtcgtgtt cggctgcctc cgccaggcga tcccggagcg
     4861 tgtgccggcc gagggcacgt cgtgcctctg gaacctcaac gtgcgcggcc agacgccgga
     4921 cgcgacgcgc ggcaactacg gtttcatgat gacggtgacg tcgaacggcg gcaccggcgc
     4981 acggccgggg aaggacggca tgtcggcgac cgcctaccct tccggcgtca agggcacacc
     5041 cgtcgagatc gccgagagca tcacgccgct gatcttctgg aagaaggagt tgcgcccgga
     5101 ttcgggcggc gccggcacct cgcgcggcgg cctcggccag gtgatcgaga tcgagagccg
     5161 catcggcatg ccgttcgacc tgctcgccgg cttcgaccgc atcgacaatc cgccgcgcgg
     5221 ccgtgacggc ggcgccaacg gcgaagccgg cagcgtctcg ctcaagtcgg gaaagccctt
     5281 gaagggcaag ggcatgcagc agatcccgcc cggcgagcgc ctcgtcatcc acacgccggg
     5341 cggcgccggc atcggcagcc ccgctacccg cccgcgcgac gccgtcgcga ccgacctcgc
     5401 cgacggcctc gtcaccgaga agacggccaa ggggatctac gccgcacctc gttgacaagc
     5461 gccccgcatc cgactacctc aagcgccaag cccagatggc ggaattggta gacgcactag
     5521 tttcaggtac tagcgctcac aaggcgtgga ggttcgagtc ctcttctggg caccattccc
     5581 tctttccgcg cagccgccac gccgatcctg cgcgcgcggc tccggttcga ggaaggacct
     5641 cgaacctccg ctttatttgg ttgcccgcgg ccgccacgcg aagcagggcg agctgtatgc
     5701 ggcgccagac atgagcgcgg ctcacaaccc cacttcgatg ttgacgacgg catttcaatc
     5761 ttgcgtgcgt cttttatagc accatgcgat tcgattttgc cgctatgctt tctctattgc
     5821 aaaattgggg aggcgaagaa tgaaaatgcg cggtgtattt gcgtcgttgc tccttgcggg
     5881 ctgcagcacg catccgatat ctgcggacgt gacactcgac acctactcaa tcgcgaccca
     5941 tgtcgaatgc gaggcctaca gggctttatc ttcggtgctc gccggatatc tcgtaaagca
     6001 cgacgaccca tatctacagc aactcggacg agatctcttc agcggccttc aaggaaaaaa
     6061 cgggcgccat ctcaacaaac tgacggttga tgagcttctg cgcttgtccg ctactgaccg
     6121 cactaagatc gtgccgtggg atgccgttcg aattggtttc ggttacacat tcaagattac
     6181 cgagaagaat gataatggcg gcagtgccgc cgtcaacgtg ccgattggta tcgctacgct
     6241 caccgtcggc gtggccgccg cagccaaccg ggcgcgtgaa tcgcttcgtg agttcgagat
     6301 cgtgatgaag ctttccgagc tcatcaagtc tcaagcctgc gatgcaccgc gagccagaga
     6361 cggaaacgcc catacttatc cgatatcagg gcagatcggg cttgttgaaa gctttggtac
     6421 atttaccgcc ctatggttga gttccggcga aaacctcaag gggcccgggc gcacaatcgg
     6481 cacgaaactc gacggattcg tcgatcaaat cacattcacg acgacgattg gcggtgggat
     6541 cacgccagag gtaacgctcg ccgctgtaaa aactggtccg cgggtcacaa aaggcacgct
     6601 tagccacgcg tcatcgagaa cggacgcaca caaactcatt ctggtcatag ttccgcctag
     6661 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnncca cgcaataatc cgtgcgcgct ggtcgggtta gtcggatcgg cataggcagg
     6841 gccccggtcg cggagcacgc ccgcgaccgc caccacgccc ctcacccctt cgccgcagac
     6901 ggccgcacca tcccgcggcc tttcatgacg tcggtgacct cgtcgaggat cacggggtcg
     6961 tcgatggtcg cgggcatctt ccagggctcg ccgtcggcga tctgacgcat ggtgccgcgc
     7021 aggatcttgc ccgagcgggt cttgggcagg cggttcacca
//
LOCUS       DAORNH010000241         7053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7902_length_7053_cov_2.681480, whole genome shotgun sequence.
ACCESSION   DAORNH010000241 DAORNH010000000
VERSION     DAORNH010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7902_length_7053_cov_2.681480"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK264_19405"
     CDS             <1..432
                     /locus_tag="PK264_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78075.1"
                     /translation="GDGHARVAADCARGDDCNDNNPASTWRAIDAEPRSLTAGDATGL
                     GKLGAVRPLEPPHTMANFVMREMGYSVARKHAEKLRSIAALLAFVVPVLLLGLGWLVS
                     MPLPAAVLAALAAGIGIIVERWLFFAEAQHVVTLYYGAERA"
     gene            635..2086
                     /locus_tag="PK264_19410"
     CDS             635..2086
                     /locus_tag="PK264_19410"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HRD78076.1"
                     /translation="MSGGIIHPVILSGGSGTRLWPLSRSMYPKQFIRFFNGHEPTFLG
                     KTLARLGSDDGFQPPILLGNNDHRFLIKEEVERSGVVTKAIYLEPVGRNTAAAIAVAA
                     LSAIRENPAAIIAVMPSDHVIQDEVRFTEAVRRAAKVAATGRLVLFGIKPTEPHTGYG
                     YIRQGKSIEGFSGAAYDVDAFFEKPDASTARTYLEAGTYYWNSGIFVLHARTFIDELS
                     RLEPRIFEAARAALGRAQEDLGFMRLDKAAFAQSPNISVDYAVMEKTHAAAMLPLDVG
                     WSDVGSWQSLWDIAPHDKSGNYVHGDAVLEDSRDCYIHTERGLVSTIGVKDLVIVDTP
                     DALLVADKARSQDVSRIVARLKQSNRKEQEQHLRNFRPWGYFETLNIGPRFQVKLLHV
                     KPGGKLSMQMHHHRSEHWIVVQGTAKVVIGDIEKLVRENESVYIFATQWHRLENPGKV
                     PVEIIEVQIGTYLGEDDIQRTDDIYHRAPEETK"
     gene            complement(2563..>3787)
                     /locus_tag="PK264_19415"
     CDS             complement(2563..>3787)
                     /locus_tag="PK264_19415"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRD78077.1"
                     /translation="NGTNGTEASPRKIRLEANIIAAEADGGRIAENIIYFARLLRAAG
                     LPVGPEKTVRATEAVLAAGLESPHVLYWTLHAVFVNRRSEREIFNQAFVMFWKDPGYL
                     QQMLSVMLPEVRPPEKEGDDKSLSRRMAESLFKSSDRPPPRKQEELDLLALETSSAEE
                     VLREKDFEQMSAEEQRRARDAIRRLRLPFEEIRTRRFERARRGERIDLRAILRATATK
                     GPDHLLPVYRRRIVRRPPIVVLCDISGSMDTYARVFLHFLYAITNDRDRVTAFLFGTR
                     LTNITRALKNRDPDAAIAKVQGEVVDWSGGTRIGTCLAEFNKRWARRVLGQNALVLLF
                     TDGLDREGGEGIDAAARRLRASCRRLVWLNPLMRYDRYEPLAAGAKALSHYASEMRPC
                     HNLSSLVGLAEALEA"
     assembly_gap    3788..3801
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3802..4596)
                     /locus_tag="PK264_19420"
     CDS             complement(<3802..4596)
                     /locus_tag="PK264_19420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78078.1"
                     /translation="MAQTSDWLGMAGRVAVVTGGASGIGRATAKSLAAAGARVAVLDI
                     NQKGAEEVAKEVGGGAIAVACDSSSEAAIAAAAKAVAGIGGADILVNNAGILKPGGLD
                     KLSLADWNAIIAVNLTGYFLMAQAFGAQMMAKRRGSIVHVASIAGQEPQTFSGAYSVT
                     KAGVVMLSRQLAVEWGPAGIRSNVVSPGLIRTPLSESFYRQPGVTEKRSAIVPRQRVG
                     TAEDIANSITFLASDKSDYVNGAEILTDGGVGSMLMTMIPRPGFERP"
     gene            complement(4716..5099)
                     /locus_tag="PK264_19425"
     CDS             complement(4716..5099)
                     /locus_tag="PK264_19425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRD78079.1"
                     /translation="MPQTINAQGTASVTTPSGLKFTDSVVGTGASPRNGQTCVMHYTG
                     WLYENGVKGKKFDSSVDRKEPFEFPIGVGMVIKGWDEGVSTMKVGGKRTLIIPPALGY
                     GARGAGGVIPPNATLLFEVELLALK"
     gene            complement(5555..>7053)
                     /gene="groL"
                     /locus_tag="PK264_19430"
     CDS             complement(5555..>7053)
                     /gene="groL"
                     /locus_tag="PK264_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602475.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRD78080.1"
                     /translation="VTVAKEIELEDKFENMGAQMVREVASKTADQAGDGTTTATVLAQ
                     AIVREGAKSVAAGANPMDLKRGVDLAVSTVVAELKAKSKKVTSNEEIAQVGTISANGD
                     KTIGSIIAEAMKKVGNEGVITVEEAKSLETELDVVEGMQFDRGYISPYFVTNAEKMIT
                     ELESPYILIHEKKLSGLQPMLPLLESVVQSGKPLLIIAEEVEGEALATLVVNKLRGGL
                     KVAAVKAPGFGDRRKAMLEDIAVLTGGTVISEDIGIKLENVTLDMLGRAKRVTIDKDN
                     TTIVDGTGKKADIEARVKQIKVQIEETTSDYDREKLQERLAKLAGGVAVIKVGGATEI
                     EVKEKKDRVDDALHATRAAVEEGIVPGGGVALLRAIKALDALKVEHEDQKVGVAIVRR
                     AIQWPARFIFQNAGEDGSVVVGKILDNSKYAFGFNAQTGEYCDLVAQGVIDPTKVVRC
                     ALQDAASVAGLLITTEAMVAELPKKDAGSPAMPGGGMGGMGGMGGMDF"
BASE COUNT         1121 a         2416 c         2267 g         1235 t
ORIGIN      
        1 ggcgacgggc acgcgcgggt agcggcggat tgcgcgcgcg gcgacgactg caacgacaac
       61 aatccagcga gcacctggcg cgcgatcgac gccgagccgc gctcgctcac cgcaggcgat
      121 gcgacgggtc tcggaaagct cggcgccgtc cgcccgctcg aaccaccgca cacgatggcg
      181 aacttcgtca tgcgcgagat gggctacagc gtcgcccgca agcacgcaga gaagcttcgg
      241 tccatcgcgg cgctgctcgc cttcgtcgtg ccggtcctgc tgctcggcct cggctggctc
      301 gtatcgatgc ccttgccagc agcagtgctg gcggcactcg cggccggcat cggcatcatc
      361 gtcgagcgct ggctgttctt cgccgaggcc cagcacgtcg tgacactcta ctacggcgct
      421 gagcgggctt gataaggagg tccagcgccg tcgactacgg cggctggaaa actcatccct
      481 tgagacctgt cgctcccact gctagaagcg cgcggttggt tcgttaatcg tttgtaaatg
      541 cggggcgtgg caccgctctc taccgagttc cgaatcgagc gggggccgat tcggccgtag
      601 cgttctgatc ctcgacgcgg cgaagaggga agccatgagc ggcggcatca tccatccagt
      661 gatcctttcg ggcggatcgg gaacgcggct ctggccgctg tcgcgctcga tgtatccgaa
      721 gcagttcatc cgcttcttca acggccacga gccgaccttt ctcggaaaga ctctcgcccg
      781 cctcgggtcc gacgacggct tccagccgcc gatcctgctc ggcaacaacg accaccgctt
      841 cctcatcaag gaggaggtcg agcgctcggg cgtcgtcacc aaggcgatct acctcgagcc
      901 cgttgggcgc aacacggccg ccgcgatcgc cgtcgcagcc ctttcggcca tccgcgagaa
      961 cccggcggcc atcatcgccg tcatgccctc cgaccacgtc attcaggacg aggtccgctt
     1021 taccgaggcc gtgcgccggg cagcgaaggt tgcggccacc ggccgccttg tcctcttcgg
     1081 catcaagccg acggagccgc acacgggcta cggctacatc cggcagggga agtcgatcga
     1141 gggcttctcg ggcgccgcct acgatgtcga cgccttcttc gagaagcccg acgcctctac
     1201 tgcccgcacc tatcttgagg ctggcacata ttactggaac agcggcattt tcgtcctcca
     1261 tgcccgcacc ttcatcgacg agctctcgcg cctcgagccg cgcatcttcg aggcggcccg
     1321 cgccgcgctc ggccgcgcgc aggaggacct cggcttcatg cgcctcgaca aggccgcctt
     1381 cgcgcagtcg ccgaacatct cggtcgacta tgcggtcatg gagaagacgc acgcggccgc
     1441 aatgctgccc ctcgatgtcg gctggagcga tgtcggctcg tggcagtcgc tctgggacat
     1501 cgcgcctcac gacaagagcg gcaactacgt gcacggcgac gccgtcctcg aggacagccg
     1561 cgactgctac atccacaccg aacgaggcct cgtctcgacg atcggcgtga aggacctcgt
     1621 catcgtcgac acgccggacg cgctcctcgt cgcagacaag gcgcgctcgc aggacgtctc
     1681 gcgcatcgtc gcccgcctca agcagtcgaa ccgcaaggag caggagcagc acctccgcaa
     1741 cttccggccc tggggctact tcgagacgct caacatcggc ccgcgtttcc aggtgaagct
     1801 cctgcacgtg aagcccggcg gcaagctctc catgcagatg caccaccacc gctccgagca
     1861 ctggatcgtt gtccagggca cggcgaaggt cgtgatcggc gacatcgaga agctcgtgcg
     1921 cgagaacgag agcgtctaca tcttcgcgac gcagtggcac cgcttggaga accccggcaa
     1981 agtgcccgtc gagatcatcg aggtgcagat cggcacctac ctcggcgaag acgacatcca
     2041 gcgcaccgac gacatctacc accgcgcacc ggaagagacg aagtagggga cacccccgtc
     2101 gcgccggagg cacgcggggt gggctcgaac cgggccgctt attgtgacgc ccacagccct
     2161 tctctgtaag ggccgcgcag gcggccccat acagctccgc gcggcgatgc ccgcacgttg
     2221 ccctgctcca ccgtcatccc gccgaagggc gggagccagg tcacgtcgga gcagtttccc
     2281 cgatccctcg cccggtgacc tgggtccccg ccttcgcggg gatgacgagt atgggatgcg
     2341 cgtctcgcgc gagcctcgcg atagccccta tcatcatcat gggtaccgaa gcggcacttc
     2401 tgccgtgcag gcgacccggg gccccgcgga atgcgcagcg caactccaaa gcactccttt
     2461 gaattcccca gtgctcggtc ctggcgcgtg gcgccggttg gcgcatcacg cggtcatgcc
     2521 agtctgtcct ccgagagcgc gatgctcgtc gagcctcgac gttcacgcct cgagcgcctc
     2581 ggcgaggccg acgaggctcg agaggttgtg gcaggggcgc atctcggagg cgtagtgcga
     2641 gagcgccttg gcgccggccg ccaacggctc gtaacggtcg tagcgcatca gcggattgag
     2701 ccagacgagg cgccggcagc tcgcgcggag ccgacgcgcg gccgcatcga tgccctcgcc
     2761 accctcccgg tcgagcccgt cggtgaagag gaggacgagc gcgttctgcc cgagcacgcg
     2821 gcgcgcccag cgcttattga actcggcgag gcaggtgccg atacgcgtgc cccccgacca
     2881 gtcgacgacc tcgccttgca ccttggcgat cgccgcgtcc ggatctcggt tcttcagcgc
     2941 gcgtgtgatg ttggtgaggc gcgtgccgaa caggaaagcc gtgacgcgat cgcggtcgtt
     3001 ggtgatcgcg tagaggaaat gcaggaagac gcgcgcgtaa gtgtccattg agcccgagat
     3061 gtcgcagagg acgacgatcg gcggacggcg cacgatgcgc cggcgataga cgggcagcag
     3121 atggtcgggc cccttggtgg ccgtcgccct cagtatcgcg cgcaggtcga tgcgctcgcc
     3181 gcggcgcgcg cgctcgaagc ggcgcgtcct gatctcctcg aaggggaggc gcaagcggcg
     3241 gatcgcatcg cgcgcgcgcc gctgctcctc ggcactcatc tgctcgaagt ccttctcgcg
     3301 cagcacctcc tcggcagacg aggtctcgag ggccaggaga tcgagctcct cctgcttccg
     3361 cggcggcggc cggtcgctgc tcttgaaaag actctcggcc atgcgccgcg aaagcgattt
     3421 gtcgtcgccc tccttctcgg gcggccgcac ctcgggcagc atgacggaga gcatctgctg
     3481 gagatagcca gggtccttcc agaacatcac gaaggcctgg ttgaagatct cgcgctccga
     3541 gcgccggttc acgaacacgg catgcagcgt ccagtagaga acgtgcgggc tctcgaggcc
     3601 ggcggcgagc acggcctcgg tcgcgcgcac cgtcttctcc ggtccgacgg gaagccccgc
     3661 agccctgagc aggcgggcga aatagatgat gttctcggcg atccggccgc cgtcggcctc
     3721 cgccgcgatg atgttcgcct cgaggcggat cttgcgcggc gaggcttccg tgccgttcgt
     3781 gccgttgnnn nnnnnnnnnn ngggccgctc gaagcccggc cgtgggatca tggtcatgag
     3841 catgctgccg acgccgccgt cggtcaggat ctcggcgccg ttgacgtagt cggacttgtc
     3901 gctcgcgagg aaggtgatcg agttggcgat gtcctccgcc gtgccgacgc gctggcgcgg
     3961 cacgatcgcc gagcgcttct cggtgacgcc cggctggcga tagaagctct cagagagtgg
     4021 cgttcggatg aggcccgggc tcaccacatt cgagcggatg ccggcggggc cccattccac
     4081 cgcgagttgc cgcgagagca ttacgacgcc ggccttcgtc acgctatagg cgccggaaaa
     4141 ggtctgcggc tcctggccgg caatcgaggc gacgtgcacg atcgagccgc gcctcttcgc
     4201 catcatctgc gcgccgaagg cctgcgccat caggaagtaa cccgttaggt tcacggcgat
     4261 gatggcgttc cagtctgcga gcgagagctt gtcgaggccg cccggcttca ggatgccggc
     4321 gttgttgacg aggatgtcgg ccccgccgat cccagcaacc gccttcgccg cggcagcaat
     4381 cgccgcttcc gaactcgaat cgcaggcgac cgcgatcgcc ccgccgccga cctccttcgc
     4441 aacctcctcg gcacccttct ggttgatgtc gaggactgcg acgcgcgccc ctgccgccgc
     4501 gaggctcttg gcggttgcgc ggccgatgcc gctcgctcca ccggtcacca ccgccacgcg
     4561 gccggccatg ccgagccagt ccgaagtctg tgccatgtcg ctgctccctg gtcgaagcgc
     4621 tgagcataat gtgaaaagag ccccgccggc tatcggcgag gcccctcatt cgggcaggtc
     4681 acatgcgcga ttgccagggc ggcaatcgct tcccgtcact tgagggcgag cagctcgacc
     4741 tcgaacagca gcgtcgcatt cggcggaatc acaccgccgg cgccacgtgc gccgtagccg
     4801 agggcgggag gaatgatcag cgtgcgcttg ccgccgacct tcattgtcga gacgccctcg
     4861 tcccagccct tgatgaccat gcctacgccg atcgggaact cgaacggctc cttgcgatca
     4921 acggagctgt cgaatttctt gcccttgacg ccgttctcat aaagccagcc ggtgtagtgc
     4981 atcacgcacg tctggccatt gcgcggcgaa gcgccggttc cgacgacgct gtcggtgaac
     5041 ttgaggccgg agggtgtggt cacggatgct gttccctggg cgttgatggt ttgcggcatg
     5101 gcgatggcga gcgtgcttgc cagcgcgaag ccgaaggcga aggccaattt cttcattcgt
     5161 tgctccttgg ccgtcgatcg ggcttcgccg tcgatcggct ccgcgaggcc atagcagagc
     5221 ggtggcctcg ctgtccgtgg aatttcgcga ctcacttcgc cgcgtgcgtg cgtttgcggg
     5281 atcactaagg cgttcgtgtg gtgcgacgct tcccgtcctc gaatgttcag ccgagacctt
     5341 cagggagtga acgatcgctc ggtgctgtcg cgagacccat gtaccatcca tccgtgcgac
     5401 ttcgactcgc ccctccacga cactgtagaa aacgagttag ctgcgaaagc acatcgctcg
     5461 acgaaagacc gccacggcga gccgtggcgg tcttgcttct ccgcagtatg cttcggatag
     5521 gccggctctt gagcccggaa atccccgcgg cggatcagaa atccatgccg cccattccgc
     5581 ccatgccacc catgccgccg ccgggcatgg ccggtgagcc tgcgtccttc ttcggcaact
     5641 cggccaccat cgcctcagtg gtgatcagga ggccggcgac agaggccgca tcctgcagcg
     5701 cgcagcgcac gaccttggtc gggtcgatca cgccctgggc gacgagatca cagtactcgc
     5761 cggtctgggc attgaagccg aaggcgtact tcgagttgtc gaggatcttg ccgacgacga
     5821 ccgagccgtc ctcaccggcg ttctggaaga tgaagcgtgc cggccactgg atcgcgcggc
     5881 gcacgatggc gacgccgacc ttctggtcct cgtgctcgac cttcagggca tcgagggcct
     5941 tgatggcgcg cagcagcgcg acgccgccgc ccggaacgat gccctcctcg acggccgcgc
     6001 gggtcgcatg cagcgcatcg tccacgcggt ccttcttttc cttcacctcg atctcggtgg
     6061 cgccgccaac cttgatcacg gccacgccgc cggcgagctt cgcaaggcgc tcctgcagct
     6121 tctcgcggtc gtagtccgag gtcgtctcct cgatctggac cttgatctgc ttcacgcgcg
     6181 cctcgatgtc ggccttcttg ccggtgccgt cgacgatcgt ggtgttgtcc ttgtcgatcg
     6241 tgacgcgctt cgcccggccg agcatgtcga gcgtgacgtt ctcaagcttg atgccgatgt
     6301 cctcggagat cacggtgccg ccggtgagga cggcgatgtc ctcgagcatg gccttgcggc
     6361 ggtcgccgaa gcctggagcc ttcacggccg cgaccttgag gccgccgcgc agcttgttga
     6421 cgacgagcgt cgccagcgcc tcaccctcga cctcctcggc gatgatgagc agcggcttcc
     6481 ccgactgcac gaccgactcg agcagcggca gcatcggctg cagtcccgag agcttcttct
     6541 cgtggatgag gatgtagggg ctctcgagct ccgtgatcat cttctcggcg ttggtcacga
     6601 aatagggcga gatgtagccg cggtcgaact gcatgccctc gacgacgtcg agctcggtct
     6661 cgaggctctt cgcctcctcg accgtgatca cgccctcgtt gccgaccttc ttcatggcct
     6721 cggcaatgat cgagccgatg gtcttgtcgc cgttcgccga gatggtgccg acctgtgcga
     6781 tctcctcgtt cgaggtgacc ttcttcgact tggccttgag ctcggcgaca acggtcgaga
     6841 ctgcgaggtc gacgccccgc ttcaggtcca tcgggttggc gccggcggcc accgacttag
     6901 cgccctcacg cacgatcgcc tgggcgagaa cggtcgcggt cgtggtgccg tcgcctgcct
     6961 gatcggccgt tttcgaggcg acctcgcgca ccatctgcgc gcccatgttc tcgaacttgt
     7021 cctcgagctc gatctccttg gcgacggtga cgc
//
LOCUS       DAORNH010000242         7021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7957_length_7021_cov_2.576371, whole genome shotgun sequence.
ACCESSION   DAORNH010000242 DAORNH010000000
VERSION     DAORNH010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_7021_cov_2.576371"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1224
                     /locus_tag="PK264_19435"
     CDS             181..1224
                     /locus_tag="PK264_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD78081.1"
                     /translation="MSITRRAVLGGTAAAVASPALVRPAAAQAVTLKLHHFLPAVSNV
                     HNKLLLPWAKKLADETKGALKVDVAPGMSLGGKLPELYDQARDGVVDMIWTLPGATAG
                     RFPSTEVFELPFVAARRGVVNARAAQEFADQHLGAETKEVQLLSYWCHDHGLIHASRQ
                     IKTMDDLKGLKLRSPTRLAGEALTALGAAGIGMPITQVPESLAQKVIDGCVVPWEVVP
                     AIKVHELVKFHTEIPGSPTLYTASFVLTMNKAKYAGLPAELKKVLDANSGQVFAGMAG
                     KMWDDAGAAVHDMVKAKGNTISSIAEDEKGRWIKATEPVIAKWIDQVKAKGLDGAKLI
                     AAAKALVAKHDKA"
     gene            1432..>2174
                     /locus_tag="PK264_19440"
     CDS             1432..>2174
                     /locus_tag="PK264_19440"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRD78082.1"
                     /translation="MAATWSPDSWRSKPIVQVPEYADKALLRDVEARLATFPPLVFAG
                     EARALKRSLGEIANGNGFLLQGGDCAESFQEHRADNIRDFFRVFLQMAIVLTYAGGQP
                     VVKIGRIAGQFAKPRSAPTEKKDGQELPSYRGDIINGPEFTTAARLPDPNRQLEAYRQ
                     SAATLNLIRAFAGGGYADLERVHQWTLGFVKGSPQSHRYEELAARITETLEFMRACGI
                     TSDNTTQLRTTTVYTSHEALLLGYEQALTR"
     assembly_gap    2175..2363
                     /estimated_length=189
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2364..2805
                     /locus_tag="PK264_19445"
     CDS             <2364..2805
                     /locus_tag="PK264_19445"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRD78083.1"
                     /translation="TLICRFGSDKIEKSLPKLINAVKRDGRKVVWSCDPMHGNTITAA
                     TGFKTRPFDRILGEVDRFFAIHRAEGTHAGGVHVEMTGQNVTECTGGAQSLTDQDLAS
                     RYHTHCDPRLNADQSLELSFLIAERLKEERAARMASTPAANAAE"
     gene            3076..3900
                     /gene="hisN"
                     /locus_tag="PK264_19450"
     CDS             3076..3900
                     /gene="hisN"
                     /locus_tag="PK264_19450"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRD78084.1"
                     /translation="MSKNSVARAAPRPATADHLAIAHTLADVSAPAIMRHFRKAVAIA
                     DKKAAFGFDPVTAADEAAERTISKYLKRHHPDHGIHGEEYGVRLGTSSLVWVIDPIDG
                     TKGFLVGSPMWGTLIGLRDGATPVLGMMNQPFTGERIWGTAKGSYWRVGDSPARRIRT
                     RACARLADAALAATHPDMFDPDALASFETVRRQAKFSRFGGDCYFYALLAAGHIDLVI
                     EAGLKPHDIVALIPIIEGAGGVVTTWTGGPATEGGRIIASGDPALHEKAMKALAAR"
     gene            complement(4810..6153)
                     /locus_tag="PK264_19455"
     CDS             complement(4810..6153)
                     /locus_tag="PK264_19455"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD78085.1"
                     /translation="MTNGGLWKSTSLAALVASVGLIAGGVSAKAADLGGDCCADLEER
                     VAELEATTVRKGNRKVSLTISGFVAHNVMFWDDGTQSDMYIGDGGNYGSRFRFVGTAK
                     ISPNVTAGFLYEFGISNNGIDAANQLNGGDDLGGGVALRDTTVWLRHSQLGMIKVGHG
                     STATDNLVLIDLSGCGGACTPDVAFYNGGMILRGAGGFASSTALTWASAIRGNAGFDT
                     ARKNHILYETPSLAGFTLQTAVAEDNYWDVALRYAGELGGFRIAGGIGYQRDSEFNAP
                     NQLFAANGALCTTHCNTVSEEIKGALSVMHVATGLFATFSAGNREVSGGLPAPGAYVG
                     PDVRYWHLTGGISNNFTGIGRTVLFGEYGEHKGGLAQSAFFASTTTHDSVSDSKVTVW
                     GLGITQHVDAAAMELFIAYKNFSLDATGFNAANAHLNGGGAADFSMVLVGTRINF"
     gene            6773..>7021
                     /locus_tag="PK264_19460"
     CDS             6773..>7021
                     /locus_tag="PK264_19460"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78086.1"
                     /translation="MAACQTDLATFCPGIEAGGGRKMKCLAENRAKLSPECGVTVDLR
                     LARKAAKGAMEETENLSTALMENLDGVRLIKIENREAAE"
BASE COUNT         1226 a         2296 c         2171 g         1139 t
ORIGIN      
        1 agcccggcct gccccaccga atcgccttcg gatcccgtca tcgtctcgtc cgccatgtcg
       61 cgatcctcct cgtgcaccgt cctcgttgtg ccctgcccgc gtggcacgtt cgtccagcgt
      121 gaaatccgcc gtattctctt gtagaacggc tctcacctaa gacttcaagg gaggaatatc
      181 atgtcgatca cacgtcgcgc cgtcctcggc ggcacagcag cggccgtcgc ctcgcccgcg
      241 ctcgtacggc cagccgcggc gcaggccgtc acgctgaagt tgcaccactt cctgccggcc
      301 gtctccaacg tccacaacaa gctgctgctg ccctgggcga agaagctcgc tgacgaaacc
      361 aaaggcgcgc tcaaggtcga cgtggcgccc ggcatgtcgc tcggcggcaa gctgcccgag
      421 ctttacgatc aagcgcgaga cggcgtcgtc gatatgatct ggacgctccc gggcgcgacc
      481 gcaggccgct tcccctcgac ggaggtgttc gagctgccgt tcgtcgccgc ccgccgtggc
      541 gtcgtcaacg cgcgcgccgc ccaggagttc gccgaccagc acctcggcgc cgagacgaag
      601 gaggtgcagc tcctcagcta ctggtgccac gaccacgggc tcatccacgc ctcccgccag
      661 atcaagacca tggacgacct gaaggggctc aagctgcgct cgccgacacg ccttgccggc
      721 gaggccttga ctgcgctcgg cgccgccggc atcggcatgc cgatcacgca ggtgcccgag
      781 agcctcgcgc agaaggtgat cgacggttgc gtcgtcccct gggaggtcgt cccggccatc
      841 aaggtgcacg aactcgtcaa gttccacacc gagatcccgg gctcgccgac actctatacg
      901 gcctccttcg tcctcaccat gaacaaggcg aagtatgcag gcctcccggc cgagctcaag
      961 aaggtgctcg acgccaactc cggccaggtg ttcgccggca tggccggcaa gatgtgggac
     1021 gatgccggcg ccgccgtcca cgacatggtg aaggccaagg gcaacaccat ctcctcgatc
     1081 gccgaggatg aaaagggccg ctggatcaag gcgactgagc ctgtcatcgc gaagtggatc
     1141 gaccaggtga aggctaaggg cctcgacggc gccaagctga tcgccgccgc caaggccctc
     1201 gtcgccaagc acgacaaggc ttgatcggct gtcgatcctc accaactggc ccgcgatgtc
     1261 gcccatcgcg ggtttaccgt ttggcgacca tcgacgcgcc gatcatcgtg cacacgcccg
     1321 gccgcgcaag cggctggcat cggcagcggc gatggcgtaa gacaccgctg ccgcacggca
     1381 tttcgccggc ggcaggactt gaggagcaac gctcatgagg agaaacgtgt gatggcggcc
     1441 acttggagcc ccgacagctg gagatcgaag ccgatcgtcc aggttcccga gtatgccgac
     1501 aaggcgctgc tcagggatgt ggaggcccgc ctcgccacgt ttcccccgct cgtttttgca
     1561 ggtgaggcgc gcgctctgaa gcggagcctc ggcgagatcg ccaatggcaa cggcttcctg
     1621 ctgcaaggcg gcgactgcgc cgagagcttc caggagcacc gcgcggacaa catccgagat
     1681 ttcttccgcg tcttcctgca gatggcgatc gtgctgacct acgctggcgg ccagccggtg
     1741 gtgaagatcg gccggatagc cggccagttc gccaagcccc gctcggcacc gaccgagaag
     1801 aaggacggcc aggagctgcc gagctaccgc ggcgacatca tcaacggtcc cgagttcacc
     1861 acggcggccc ggttgccgga cccgaaccgc cagctcgagg cctatcgcca gtcggccgcg
     1921 acgctgaacc tgatccgcgc cttcgcgggc ggcggctatg ccgacctcga gcgcgtgcat
     1981 cagtggacgc tcggcttcgt caaagggagc ccgcagagcc atcgctacga ggaactcgcg
     2041 gcacgcatca ccgagacgct cgagttcatg cgcgcctgcg gcatcacctc cgacaacacg
     2101 acacagttgc gcaccactac ggtctacacg agccacgagg cgctgctgct cggctacgag
     2161 caggcgctga cccgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnncacgctc atctgccgtt tcggctcgga caagatcgag
     2401 aagtcgctgc cgaaactcat caatgccgtg aagcgtgacg gccgcaaggt cgtctggtcg
     2461 tgcgatccca tgcacggcaa tacgatcacg gcggcaacgg gcttcaagac gcggcctttc
     2521 gatcgcatcc tcggcgaggt cgatcgcttc ttcgcgatcc atcgggcgga ggggacgcat
     2581 gccggcggcg tgcatgtcga gatgacgggg cagaacgtca ccgagtgcac gggcggcgcg
     2641 cagtcgctga ccgaccagga cttggcgagc cgctatcaca cgcattgcga cccccggctc
     2701 aatgccgatc agtcgctcga gctctcgttc ctgatcgccg agcggctgaa ggaggagcgc
     2761 gcggcgcgga tggccagcac gccggcggca aatgccgccg agtaggccgg cacaaaaaaa
     2821 gggccgtccg gttgggcggc ccaagtccag ggaggaaacg cccaacgcgg gcagtggagc
     2881 cgcatccgag cggcaaccac ccatccctct tgatccgttc tccgagtcgc agcaagcggc
     2941 ttcgctggat caccgccaat ccatcacctt cgcgtagctt ttccaacaca gtcgacgaaa
     3001 gtctcgtgac gaggcccggc acgcgaaccg gtgcgacagg ctcttgcgat gacgtcgatg
     3061 cttgtggagc aggaaatgtc caagaattca gtggcccgcg ccgcaccgcg acccgccacc
     3121 gccgatcatc ttgcaatcgc gcacacgctc gccgatgtgt cggcgccggc catcatgcgc
     3181 catttccgca aggcggtggc catcgccgac aagaaggccg ccttcggctt cgatccggtg
     3241 acggccgccg acgaggcggc agaacgcacc atctcgaagt acttgaagcg ccatcacccc
     3301 gatcatggca tccacggcga agaatatggc gtccgcctcg gcacgagcag cctcgtctgg
     3361 gtgatcgatc cgatcgacgg caccaagggc tttctggtcg ggagccccat gtggggcacg
     3421 ctcatcggac ttcgcgacgg ggcgaccccg gtcctcggaa tgatgaacca gcccttcacc
     3481 ggcgagcgca tctgggggac ggccaagggc tcgtattggc gggtcggcga cagtccagcg
     3541 cgccgcatca ggacgcgcgc gtgtgccaga ttggcggatg ccgccctcgc cgccactcac
     3601 cctgacatgt ttgacccgga cgccttggcg agcttcgaga ctgtccgtcg ccaggcgaag
     3661 ttctcgcgct tcggcggcga ctgctatttc tacgcgctgc tggccgccgg ccacatcgat
     3721 ctcgtgatcg aggccgggct caagccgcac gacatcgttg cactcatccc gatcatcgaa
     3781 ggggccggcg gggtggtaac gacgtggact ggtggccccg cgactgaggg cgggcggatc
     3841 atcgcgtcgg gtgaccccgc gcttcatgag aaggccatga aggcgctcgc ggccaggtga
     3901 gcaagaacaa atgaagttcg agggcgccgc gcgtccaagc ggagcttggc tccgccaaga
     3961 cgaactcccg ccggggattg atccccatac gcgccaacat agccggggca gatttcgatg
     4021 ggcgcgcttt cgcgggggct cgcggaggtg gcgttggttg gttggggcgg catgttgcgc
     4081 gagcgtgggc tggtgagcga tcgccacgag cagacccttc gccgcgaggg aggtcagacg
     4141 ccagacggtc agcgcggaca ggcggcgagg cgaggctggc ccgggacgtg agagtcctcg
     4201 accaccgcgg tgtgcggctc gagcagcgag tccggcagaa gatgcgcgag actccaggct
     4261 ttagcgatct ggcgtcctcg cccggcggcc cggcgaggcc aatgaggcga ttgcggcgct
     4321 tgaagcccat ctctgtggta cggcctctcc gagagacgag atcgtctcgc acgtccctac
     4381 gttcggtcca tcgccgcctt ccggccgcta cttcaaactc tgctgacttc ccccgcagtc
     4441 gatacgcggc cgtcgccctt tttgggcgcg aggggagatc tctccgggta agatcgccga
     4501 tcttcgttgc gcgaccgccg gatccacgtc gcctgggcct tggccacgag agcttcgcga
     4561 tttttggcgc gctcgccctg cccgacgccg cctcatttcc gattcctgtg cgtccgcccg
     4621 caacgtcgct cccagcttcc ttcacgccgt cctcgcggtc cggcgctctg cggttcaata
     4681 agctcgttgt gacctgctca cgcgaggact ttcaatctca gatcagcgac cgtcccgggc
     4741 gcacaacgca agagagccgc ccgaaggcgg ctctcgagag actggctgga ctgccctgtc
     4801 agatcggcat cagaagttga tgcgggtgcc gacgaggacc atcgagaagt cggccgcgcc
     4861 accaccattg aggtgggcgt tggcagcgtt gaagcccgtc gcgtcaagag agaagttctt
     4921 gtacgcgatg aagagctcca tggcggctgc atcgacgtgc tgcgtgatgc cgaggcccca
     4981 gacagtgacc ttgctgtcag acaccgagtc atgcgtggtc gtggaggcga agaacgccga
     5041 ctgcgccaga ccacccttgt gctcgccgta ctcaccgaac agaaccgtgc ggccgatgcc
     5101 ggtgaagttg ttcgagatac caccggtcag gtgccagtag cgaacgtccg gaccgacata
     5161 cgcgccggga gccggaagac cgccggacac ttcacggtta ccagcagaga acgtggcgaa
     5221 cagaccggtc gcaacgtgca tgaccgagag ggcgcccttg atctcttcgg agaccgtgtt
     5281 gcaatgcgtc gtgcaaagcg cgccattagc cgcaaacagc tggtttggcg cattgaactc
     5341 gctgtcacgc tggtagccga tgccaccggc gatgcggaag ccaccgagct cacctgcgta
     5401 acgcagtgcg acatcccagt agttgtcctc ggcgacggcc gtctgcagcg tgaagccggc
     5461 gagcgacggg gtctcgtaca ggatgtggtt cttgcgagcc gtatcgaagc ccgcgttgcc
     5521 gcgaattgca ctggcccaag tcagcgcagt cgagctcgca aagccaccgg caccacgaag
     5581 gatcatgccg ccattataga acgccacgtc aggggtgcag gcgccgccgc atcccgagag
     5641 gtcgatcagc acgaggttgt ctgtcgcagt cgagccgtgg ccgaccttga tcataccgag
     5701 ctggctgtga cgcagccaca cggtcgtgtc gcggagggca acgccgccgc cgaggtcatc
     5761 gccgccgttc agctggttcg cggcatcgat gccattgttc gaaatgccga actcgtagag
     5821 gaagccggca gtcacgttcg gcgagatctt ggccgttccg acgaagcgga agcgcgagcc
     5881 gtagttaccg ccgtcaccga tgtacatgtc cgactgggtg ccgtcgtccc agaacatcac
     5941 gttgtgggca acgaaaccag agatcgtgag cgacaccttg cggttgccct tgcggaccgt
     6001 cgtcgcctca agttcggcaa cgcgctcctc gagatcggcg cagcagtcgc cgccgagatc
     6061 ggcagccttg gcgctgacac cacctgcgat caagccgact gacgcaacca gcgccgccag
     6121 gctcgtcgac ttccaaagtc ccccatttgt catgcgactc tccactcgac ttgacctaac
     6181 ccgacttatt tggaccagac ttgcttacgc ccgtctcagt cttgtctccc gatgtccggc
     6241 gcggggcctt cgatcgaacg cttcacttca gttccagtcg attacagcga ttgctcagtc
     6301 cgtgcgattg ccttcacttc ctcgacttgg tcgcctggtc caaaccggcc gggggcgacc
     6361 acctcggctc cacctgggat aagatcccgg ccggcgcctc cggtagtcgt agctcgtcga
     6421 ttcgacccgc gtccaatccg tttttggttt tgcccggctt ttgagcggtt cgcgtggcac
     6481 gaaagcaact gtgactgccg ggcgcctgcg acgaactgga ccctacggct cgtgcgaccg
     6541 ggttgccggc tgatattggt cgcccgagct tagctcttcg gccgaccgcg acggaagccg
     6601 cccccgcaac cgttcttggc atgctcacgg ttaacacagt cggaccgggc tcgccacgaa
     6661 atcataaggg aaatcaacat gtcactttca atgtcgctac gcgtcgggct gacgttggcg
     6721 ccgctcgccg tggtcgcgct cgcgccctgg gcttcagccc aggcgcggtc gcatggccgc
     6781 ctgccagacg gacctcgcca cgttctgccc gggcatcgag gccggtggcg gccggaagat
     6841 gaaatgcctc gccgagaacc gggcgaagct ctcgcccgag tgcggcgtca ccgtggacct
     6901 ccgccttgcg cgcaaggccg ccaagggcgc gatggaggag accgagaacc tgtccaccgc
     6961 cctgatggag aacctggacg gggtgcggct gatcaagatc gagaaccgcg aggcggccga
     7021 g
//
LOCUS       DAORNH010000243         7008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7986_length_7008_cov_2.062275, whole genome shotgun sequence.
ACCESSION   DAORNH010000243 DAORNH010000000
VERSION     DAORNH010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7986_length_7008_cov_2.062275"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>438
                     /locus_tag="PK264_19465"
     CDS             <1..>438
                     /locus_tag="PK264_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78087.1"
                     /translation="MAEGGAVARSAIERAIETLDLTALSGRPADRLSGGELARVLLAR
                     ALAQEAAILIADEPTASLDPAHQIAVFDELRRLARGGSTVIAALHDLSLTARYADEVW
                     IIAGGSVRASGTPDVVLTADVLSDVFAIPMQRGEIGGVPAIVA"
     assembly_gap    439..448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(535..>1346)
                     /locus_tag="PK264_19470"
     CDS             complement(535..>1346)
                     /locus_tag="PK264_19470"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRD78088.1"
                     /translation="DPKGADPKAADPKTTDPKAADPKPADTKPGDTKAADTKADPKAD
                     PKTASAPAAPVEPPLRNPGRYGIIGLGTGSVGCHAKPGEKWRFFEIDPAMMKISTNPE
                     NFSFLTNCHKGNPDIVIGDARLTMAKEPDKSFDLIIVDAFSSDAVPVHLMTKEALEIF
                     KAKIKDKGVFVLHVSNRHLDLEGVLSATAPLVPGIHGLVITDNKSESYFSSSSTVVVF
                     AKEPSVLEDFRELANRIKTTTVGELNARTLKPWTDDWSDIVGPYMSRHQGN"
     assembly_gap    1347..1479
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1480..3416)
                     /locus_tag="PK264_19475"
     CDS             complement(<1480..3416)
                     /locus_tag="PK264_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78089.1"
                     /translation="MRSDPYFLYGASNLGSFLALLGYPLVLEPYLGLKSMSRWWTIGF
                     VALIAAIALSLWYVRSSEAPRAAGSGKPSDVDADTAAPTWGQRLGWIGLAFVPSALLT
                     AFTTHVSTDVASAPLIWVVPLAFYLLTFVLVFRDRPIILVPAIIMAALLAHNLLPATV
                     YRLSYTYRYAMSDSVHIYSVLAVALALAVAYLSAGYPRRLGMVLGSIIAATGAFAVIH
                     LIGGAGQEHHAAFIAIVAGLLFAAWHGGLLERKLFEILAGVVGATAIVMFGLPLLYKY
                     FVGGEMQAMSRIVLALMAGATALAAIMQRAGDEHAADRTWWMGLIHLVSVVATLFFLA
                     PTGGPGWWTLIVLGSIVFFSSTMVAHRTLFELRPAAKHLTSFYLLMSLGGGLGGLFTA
                     LIAPQIFKEVYEYPLLLALSMFCRPGVSLAPLGSFVAWIGHKGGILRNSSEPEFSEAK
                     ANELMVQWLLLAGGLLVLFWVPWILQRNPVVYQVLDQYLELESVLGLLSVLALGGAVF
                     VWLQKPQFRQLAVAVGIVAGVFLISAWLRNPFRQWDTLGAISGAFALVLFLCWKFPTR
                     QAIMALLMGVAVVWHYSGVKQDGVATRSFFGVYRVHVARDGRAGEANRFMVLQHGTTL
                     HGAQRRARPVRCRSGKFRAVP"
     assembly_gap    3443..3527
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3534..4133)
                     /locus_tag="PK264_19480"
     CDS             complement(3534..4133)
                     /locus_tag="PK264_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78090.1"
                     /translation="MSKKNKHRNKGRPQGKPAVQPPKPTPAPAATASATAPTTSSAAS
                     YAAAAREASKVSHVGDALPVSPASAPMARPVPRPVSQPARASKAAPTVGLMSLSQGDW
                     LTLLVFSATTFLSAVLLFSIQPMFAKMVLPVLGGSPSVWAVAMCFFQGALLAGYCYAH
                     ILISRFQPKLAGLIHLGVCAVAFIVLPIAMPASWGEKRR"
     gene            complement(4275..>4723)
                     /locus_tag="PK264_19485"
     CDS             complement(4275..>4723)
                     /locus_tag="PK264_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRD78091.1"
                     /translation="SWTRSPLFSFCRSSGGTVRSADFDLTLVNALHSSSSGGVYLGNP
                     NGIVLTTREGRTVYHMGDTDMFGGMALIAELHRPDVGIVPIGDRFTMGARSAAFACRK
                     FFKFKTILPCHYGTFPGMLDPTADQFIAEMKGENVRAPKIGETISP"
     assembly_gap    4724..4733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4734..5014)
                     /locus_tag="PK264_19490"
     CDS             complement(<4734..5014)
                     /locus_tag="PK264_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD78092.1"
                     /translation="MKIIWFGHSCFRIETGSSVILIDPFLKGSPTFKASGIAFEAATK
                     GVTHVAVTHGHDDHIGDAGEICKTNGATLFANFELAMHIKGKGAEKLEP"
     gene            5178..5684
                     /gene="ruvX"
                     /locus_tag="PK264_19495"
     CDS             5178..5684
                     /gene="ruvX"
                     /locus_tag="PK264_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRD78093.1"
                     /translation="MTRASPQVATLDPDAFAALLGRGERLIGIDVGTDTLGLALSDVE
                     RMVATGLMTIRRTRFGRDADALLALVAEHRVGGLVIGLPIHLDGGEGPRAQATRAFAR
                     NLAQRTPLPMLLWDERMTTVAAERALLEADTSRKRRAALIDQVAATLILQTALDRMRP
                     RPAAVPAD"
     gene            5811..>5994
                     /locus_tag="PK264_19500"
     CDS             5811..>5994
                     /locus_tag="PK264_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD78094.1"
                     /translation="MFRFRMLAGAALAAAVAASAPLMLPGPAASQAPYPSRPITLIVP
                     WPAGGSTDLVMRAMAEV"
     assembly_gap    5995..6060
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6061..6791
                     /locus_tag="PK264_19505"
     CDS             <6061..6791
                     /locus_tag="PK264_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HRD78095.1"
                     /translation="MAAAAKPDGYTISQIPITVMRLPLMEKRTWDAKRDFTYIIHLTG
                     YTFGITTKADGPFKTWEDVVAYAKANPKKVSYATPGTGTTLHIGMEMIAAKAGIQLTH
                     VPFKGGAETSTAVLGGHTTLQADSTGWKPLVDSGQLRLLALWTPARSKNWPETKTLKE
                     LGYPYVFDSPFGIAGPKGMDPAHVAKLHDAFKKALDDPSVLAMMAKYDMFPRYMGPQD
                     YVKFVDEISEQERKALEGLGLVKK"
BASE COUNT         1256 a         2169 c         2218 g         1061 t
ORIGIN      
        1 cgatggccga aggcggcgcg gtggcccgct cggcgatcga gcgggcgatc gagacgctcg
       61 atctgacggc gctctcgggg cgaccggcgg acagactgtc cggcggtgaa ctcgcccgcg
      121 tgctcctcgc tcgcgccctc gcgcaagagg cggcgatcct gattgccgac gaaccgacgg
      181 cgagcctcga tccggcgcat cagatcgccg tattcgacga gttgcggcgg cttgcccggg
      241 gcggttccac cgtcatcgca gcgctgcatg acctctcgct cactgcgcgc tatgccgacg
      301 aggtctggat cattgccggc ggcagtgtga gagcttcggg gactcctgat gtggtgctga
      361 ccgccgacgt gctctcggac gtattcgcca tccccatgca gcggggcgag atcggcggtg
      421 ttccggcgat tgtggcggnn nnnnnnnncc gaacgcccgc acaccaggtg atggtcaagc
      481 gaaggccccg caccgcatcg cggctagggc cttcgtcgag cagagtgtgc gccgtcagtt
      541 gccctggtgg cgcgacatgt aggggccgac gatgtcggac cagtcgtccg tccaaggctt
      601 caaggtccgc gcattgagct cgccgacggt cgtcgtcttg atgcgattgg cgagctcacg
      661 gaagtcctcg agcacggacg gctccttggc gaacaccaca actgtcgatg acgacgagaa
      721 gtagctctcg ctcttgttgt ccgtgatcac caggccgtgg atgccaggca ccagcggcgc
      781 agtcgccgac agcacgccct cgagatcgag atggcggttc gagacgtgca gcacgaagac
      841 gcccttgtcc ttgatcttcg ccttgaagat ctcgagcgcc tcctttgtca tgaggtgcac
      901 cggcaccgca tcggaggaga acgcgtcgac aatgatgaga tcgaacgact tgtcgggctc
      961 cttggccatg gtgagacggg cgtcacctat gacgatatcc ggattgccct tgtggcagtt
     1021 cgtcaggaag ctgaagttct cggggttggt cgagatcttc atcatcgccg gatcgatctc
     1081 gaagaaccgc cacttctcac cgggcttggc atggcagcca accgagccgg tgccgaggcc
     1141 gataatgccg tagcggcccg gattgcgcag cggcggctcg accggcgcgg caggcgccga
     1201 agccgtcttt ggatcggcct tcgggtcggc cttggtgtcc gccgccttgg tgtcacccgg
     1261 tttagtgtcc gcaggcttag gatcagcagc ctttggatcg gtagtcttcg ggtcagcggc
     1321 cttggggtcg gcgcccttcg gatcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgggaacggc tcgaaacttc
     1501 cccgaccgac accgaactgg ccgcgcgcgt cgctgcgcgc cgtgaagcgt cgtgccgtgc
     1561 tggagcacca tgaagcggtt ggcctcgcct gcccggccgt cgcgcgcgac gtgcacgcga
     1621 tagacgccga agaagctccg cgtggcgacg ccgtcctgct tcacgcccga gtagtgccac
     1681 acgaccgcga cgcccatgag gagcgccatg atcgcctgcc gcgtcgggaa cttccagcac
     1741 aggaacagca cgagcgcgaa ggcgcccgag attgcgccga gcgtgtccca ttggcggaag
     1801 ggattgcgca accacgccga aatcaggaat acccctgcga cgattccgac cgcgacggcc
     1861 agctgccgga actgcggctt ctgcagccac acgaacaccg cgccgccgag tgccagcacc
     1921 gagagcaggc cgagcacgga ctcgagttcg aggtactggt ccaacacctg gtagaccacg
     1981 ggattgcgct gcaggatcca cggtacccag aagagcacca gcagtccgcc ggcgagcaga
     2041 agccactgca ccatcagctc gttggccttc gcctccgaga attcgggctc ggaggagttg
     2101 cgcaggatgc cacccttgtg cccgatccag gcgacgaacg agccgagcgg ggcaagcgac
     2161 acgccggggc ggcagaacat cgagagcgcc agcagcagcg ggtactcgta gacctccttg
     2221 aagatctgag gggcgatgag agcggtgaag aggccgccga gcccgccgcc gagcgacatg
     2281 aggagataga agctcgtcag gtgtttggcc gcagggcgca gctcgaacag cgtccggtgc
     2341 gccaccatgg tggacgagaa gaacacgatg ctgccgagga cgatcagcgt ccaccagccg
     2401 gggccacctg tcggcgcgag gaagaacagc gtcgccacca ccgacaccag atggatgagc
     2461 cccatccacc acgtccggtc ggcagcgtgc tcgtcgccgg cacgctgcat gattgcagcg
     2521 agcgccgtgg caccggccat gagtgccaaa acgatgcgcg acatcgcctg catctcgccg
     2581 ccgacgaagt acttgtagag caagggcagg ccgaacatca cgatcgcggt tgcgccgacg
     2641 acgcccgcaa ggatctcgaa gagcttccgt tcgaggaggc ccccatgcca ggcggcgaac
     2701 aagagaccgg ccacaatcgc gatgaacgcc gcgtggtgct cctggcccgc gccgccgatg
     2761 agatggatga cggcaaaggc acccgtcgca gcgatgatcg agccgagcac catgccgagg
     2821 cggcgcggat agccggcgct cagataggcg accgccagcg ccagtgcgac ggcaagcacg
     2881 ctgtagatgt gcacgctgtc gctcatggca tagcgatagg tgtagctcag ccgatagacg
     2941 gtagcgggca gcagattgtg tgcgagtagt gccgccataa tgatggccgg cacgaggatg
     3001 atcggccggt cgcggaagac gagcacgaaa gtcagcaggt agaaggcgag aggcacgacc
     3061 cagatgagcg gggccgaggc gacgtccgtc gagacgtgcg tcgtgaaggc cgtcagcagg
     3121 gccgacggaa caaaggcgag gccgatccag ccgagacgct ggccccaggt cggcgccgcc
     3181 gtgtcagcgt cgacgtcgct gggcttaccg gacccggcag cacgcggcgc ttcgctcgag
     3241 cgcacgtacc agagcgagag cgcgatggct gcaatcagcg cgacgaagcc gatcgtccac
     3301 cagcgcgaca tcgacttgag gccgagataa ggctcgagca ccagcgggta gccgaggagc
     3361 gcgaggaagc tgccgaggtt cgatgcaccg tagaggaagt acgggtccga gcgcatcggg
     3421 tggccggtgt tcgagaacca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg cgcctaccgg
     3541 cgtttctcgc cccagctcgc cggcatggct atcggcagca cgatgaaggc gacggcgcag
     3601 acaccgagat ggatgagccc ggcgagcttc ggctggaagc ggctgatcag gatgtgcgcg
     3661 tagcagtagc cggcaagcag cgcgccctgg aaaaagcaca ttgcaactgc ccacaccgac
     3721 ggcgagccac cgagcacggg cagcaccatc ttggcgaaca tcggctggat ggagaacagc
     3781 aacacggccg acaggaacgt cgtcgccgag aagacgagca gcgtgagcca atcgccctgc
     3841 gacagcgaca tcaagccgac agtcggcgcg gctttcgatg cacgggcggg ttgtgacacc
     3901 gggcgcggca cgggccgcgc catgggcgcg gacgccggtg acaccggcag cgcatcgccg
     3961 acgtgggaca ccttggaagc ctcccgcgcc gccgccgcgt aactggcagc actcgaggtc
     4021 gtgggcgccg tcgcgctcgc cgttgccgcc ggagccggtg tcggcttcgg cggctgaact
     4081 gctggcttgc cctgcggcct gcccttgttg cggtgcttgt tcttcttcga catctcgcgt
     4141 caagccttct cagagcgaac ggggaggaac aacctgcctg cacgtggcac agcgatttgc
     4201 agcgtcgcag tcgtcgtgcg gtcgatgcag ggcggttgtc gtcaagcaag gaggaacatc
     4261 tgtggccggg gggctcaggg tgaaatcgtc tcgccgatct tcggtgcgcg aacgttctcg
     4321 cctttcatct cggcaatgaa ctgatcggcg gtcggatcca gcatgccggg gaaggtgccg
     4381 tagtggcaag gcaggatggt cttgaacttg aagaacttgc gacaggcgaa ggccgccgac
     4441 cgagcgccca tggtgaagcg atcgccgatc ggcacgatgc caacgtccgg ccgatgcaac
     4501 tcggcgatca gcgccatgcc gccgaacatg tcggtgtcgc ccatgtgata gacggtgcgg
     4561 ccttcgcggg tcgtgagcac gatgccgttg ggattgccga ggtaaacgcc gccggacgac
     4621 gaggaatgta gcgcgttgac gagggtgaga tcgaagtccg ccgacctgac ggtgccgccc
     4681 gaagacctgc aaaagctgaa cagcggcgac ctggtccagc tccnnnnnnn nnnatcggct
     4741 cgagcttttc agcacctttg cctttgatgt gcatggcgag ttcgaagttc gcgaacagcg
     4801 tcgcgccatt cgtcttgcag atctctcccg cgtcaccgat gtgatcgtcg tgcccatgcg
     4861 tcaccgccac gtgcgtgacg cccttcgtgg ccgcctcgaa cgcgatcccc gacgccttga
     4921 atgtcgggct gcccttcagg aacgggtcga tcaggatcac gctgcttccg gtctcgatcc
     4981 tgaaacagga atggccgaac catatgatct tcatatattc gtctccacgc gcacaggcgg
     5041 attggctcgg gcgcgcagta taagcaccgc gcgtccgaca acaaccgcaa tcgagagcca
     5101 agatcgccgt cccatgatcg tggttcgaat acctgaccac cgcgacaggc tcgtcacgcc
     5161 tcttgcccgg agatgacatg acccgagcct cgccgcaagt cgcgaccctc gatccggatg
     5221 cgttcgccgc cctgctcggc cggggcgagc ggctgatcgg catcgacgtc ggcaccgaca
     5281 cccttggcct cgcgctttcg gacgtcgagc gcatggtagc gaccggcctc atgaccatcc
     5341 ggcggacccg cttcggtcgc gacgccgatg cccttctggc gctggtcgcc gagcatcgcg
     5401 tcggtggcct cgtcatcggc ctgccgatcc atctcgacgg cggcgaaggt ccgcgcgccc
     5461 aggcgacacg ggcctttgca cgcaatctcg cccagcgtac cccgctccca atgctgctct
     5521 gggacgaacg catgacgacg gttgccgcag agcgcgcgct gctcgaggcc gatacgtcgc
     5581 gaaagaggcg cgctgccctg atcgatcagg tcgccgcgac cctcatcctg cagacagcgc
     5641 tcgaccgcat gcggccgcgt cctgctgccg tcccggccga ttgagcccca gcagcagcga
     5701 aactccctcg ccacacacaa aggcccgctc caatcagttg cttcttgcca agccttgcga
     5761 ggcgggacac tctttggacg aggggctgaa aatcatggga ggagttccga atgttccgtt
     5821 tccgcatgct ggccggggca gcacttgcag cagccgttgc cgccagcgcg ccgctgatgc
     5881 tgcccggccc ggccgcaagc caggcgccgt atccatctcg accgatcacg ctcatcgtac
     5941 cttggcccgc gggcggttcg actgatctcg tcatgcgcgc gatggccgag gtcgnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 cgatggcggc agccgcaaag cccgacggct acaccatcag ccagatcccc atcaccgtga
     6121 tgcgcttacc gttgatggaa aagcggacgt gggacgccaa gcgcgacttc acttacatca
     6181 tccatctgac gggatacacc ttcggcatca cgacgaaggc tgacggccca ttcaagacct
     6241 gggaggacgt cgtcgcctat gccaaggcga acccgaagaa agtcagctac gccacgcccg
     6301 gcacggggac cacgcttcac atcggcatgg agatgatcgc cgcgaaggcc ggcatccagc
     6361 tcacgcacgt accgttcaag ggtggcgcgg agacgtcgac ggcagtgctt ggtggtcaca
     6421 ccactctaca agccgattcg accggctgga aaccgctcgt cgatagcggg cagctgcgcc
     6481 tcctcgcact ctggacaccg gctcgcagca agaattggcc cgagaccaag acgctgaagg
     6541 agctcggtta tccttacgtg ttcgacagtc cgttcggcat tgctggaccc aagggcatgg
     6601 accccgccca cgtcgccaag cttcacgacg ccttcaagaa ggcgctcgac gacccaagcg
     6661 tgctcgccat gatggccaag tacgacatgt tcccgcgcta tatggggccg caggactatg
     6721 tgaagttcgt cgatgagatc agcgaacagg aacgcaaggc gctcgagggg ctcggccttg
     6781 tgaagaagtg actcgagtgg acgacatggt caggtgtcag ggagggcagt tcgcgtgacc
     6841 gttgtctgcc caccatcccg atccagcatg tggcccgcac gcgtgtccgg ccggaggcat
     6901 gagcctccgg ccggagatcg tttcggcgtc gctgcgtcag gcgcgctacc ctcacctcgc
     6961 aaagaccggc gggcgcaccg ggcgcaggtg cggcggcgcg accggctt
//
LOCUS       DAORNH010000244         7006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_7990_length_7006_cov_2.461948, whole genome shotgun sequence.
ACCESSION   DAORNH010000244 DAORNH010000000
VERSION     DAORNH010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7990_length_7006_cov_2.461948"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PK264_19510"
     CDS             <1..819
                     /locus_tag="PK264_19510"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRD78096.1"
                     /translation="YKQPVVGEGALVIDLEDGVAPGAKPKARENMRAALVAREARRSE
                     RIVRINAMTSPESSADLGALPIASVDTVFVPKVESRAEIERLDADLSRCERAHGYEGH
                     PPIGIIATIETPRGLWRALEIADASPRVTALFFGSGDYTLATGASVTERALAGPRALV
                     VAAAAAAGIEAIDAAYFLDVKDQSAVAADARIARELGFKGKLVFHPSQIAPVNEIMSP
                     SADEIARARRIRAAFDSAAAEGRGTAVIDGQFIAIDIALMADRVLARAAALGLA"
     gene            complement(835..>1883)
                     /locus_tag="PK264_19515"
     CDS             complement(835..>1883)
                     /locus_tag="PK264_19515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRD78097.1"
                     /translation="ITGVSRDKIILHADRFSWFAAAAADEAIKQSGLKVPFENGSRAA
                     CIVGSGAGGLVTYEKAYRDLFIHNKRATHPLTLLRIIGSSASAHVGIEFGVKGPTFAT
                     CSACSTATHAVGIARDYIQQGLVDVAIAGASESVINYGTMKAWQALHVLSPQGVFPFA
                     KKRNGTVLGEGAGILVLEEYEHAKRRGAKILAEICGYGMTSDSKDMVNPDTEGPREAM
                     RLALADAGLEPTAIDYVNAHGTATTINDKNETNAIKEVFGPHAYKLAVSSTKSMHGHP
                     LGAGGGIEAVACIKAMEEGWMPPTINLDEVDPECDLDYIPNVGRAKKLTYTMSNSFAF
                     GGLNAVLVFGPPPG"
     assembly_gap    1884..1947
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1948..2074)
                     /locus_tag="PK264_19520"
     CDS             complement(<1948..2074)
                     /locus_tag="PK264_19520"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRD78098.1"
                     /translation="MSTPRRRVVVTGLGVVTPIGLNVTEFWSGMRKGQCGVTALEG"
     assembly_gap    2407..2416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2417..2642)
                     /locus_tag="PK264_19525"
     CDS             complement(<2417..2642)
                     /locus_tag="PK264_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRD78099.1"
                     /translation="MDEVAKKIIEILRKHMKEPRDDISRDTSLMDLKIESLDLALIVF
                     DIEEAFGIEIPYNANEEAADFKTVGSVVDRV"
     gene            3013..>4230
                     /locus_tag="PK264_19530"
     CDS             3013..>4230
                     /locus_tag="PK264_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD78100.1"
                     /translation="MSNGGAHANGAAHGHSHYPWLANYPKAVNWHQTFKAEPLHRLLE
                     TPAGKYPSRICTNFLGKALTYRDIASLVDKAAAGLQKLGVVKGTRVGLFLPNSPTFII
                     YYFAILKAGGTVVNYNPLYTLDELTFQVKDSGTELMVTLDLGLLFGKVESLIAAGTLK
                     RAIVASFRGLLPTAKSVLFRLFKGKELAPIASSRVRDRIILEADVLANDGKPSPVAID
                     VMNDIAVLQYTGGTTGTPKGAMLTHANVHINVQQVMAWAPELKEAEERVLGVLPFFHV
                     FAMSVVMNFGIAKACEIVIMPRFVLDDALKLITSAKPTVMPGVPTLFNAMMNHPKLKS
                     FNLSSLKFCLSGGAALPLDVKQNFEALTGCKLVEGYGLSEASPVVTCNPLDGPVKKNS
                     IGQPLPATIVSLRD"
     assembly_gap    4231..4240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4241..4750
                     /locus_tag="PK264_19535"
     CDS             <4241..4750
                     /locus_tag="PK264_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD78101.1"
                     /translation="PAEPTREVPQGDKGEVCIKGPQVMKGYWNKPDETANQFVGDFLR
                     TGDVAYMDEDGFLFIVDRIKDLIISSGYNVYPRVIEEAIQLHPAVEEVTVIGITDKTR
                     GEVPKAFIKLKSGTPATADEIMKFLSEKISKREMPTEIEFRDALPKTMIGKLSKKELK
                     AEEAQRAKG"
     gene            4753..5214
                     /locus_tag="PK264_19540"
     CDS             4753..5214
                     /locus_tag="PK264_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRD78102.1"
                     /translation="MGKRTGRRLDDGEASSSRGARSEKGSKNRNRPADAFTISDLSAE
                     FAVTTRTIRFYESEGLISPTRRGSKRIYSRRDRRRLILILRGKNLGFSLEEIAEYLAL
                     YDADPSGVTQLKHLIGKVDAAIDELNRKRTDLDRTLRELKDIQAKARELLG"
     gene            complement(5280..>7006)
                     /locus_tag="PK264_19545"
     CDS             complement(5280..>7006)
                     /locus_tag="PK264_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="N,N-dimethylformamidase large subunit"
                     /protein_id="HRD78103.1"
                     /translation="TGQSHGLRTSLDGVPRLDGPVMRIAASGGAEARHHFNGKIEAPA
                     LFARGLTDTEIDDMAAGRVPAGLVAAWDFSRDVSSMRIVDTGPAKLDGHLVNLPTRAM
                     AGATWSGREMCWRHAPAEYAAIHFHDDDIYDCGWSTDFTWTVPTGTRSGNYAVRLKAG
                     DAEENIPFFVVPEKGKRTADVVVLVSTFTYTVYHNHTRPEWNTNPAWREAWQSQAKAW
                     GAYPHNPGANREYGLSTYNYHTDGSGIAYASWLRPMLTVRSGYITYPDESVRGSGLRH
                     MPADTHLTAWLEAKGIAYDVITDLELDEEGIDVLRPYRVVMTGSHPEYHTPRMLDALS
                     AYRDGGGRLMYMGGNGFYWKIARSKHLPAAIEIRRAEGGIRAWAAEVGEYYNAFDGEY
                     GGMWRRNGRPPQNLVGVGFTAQGNFVGSHYRVKAAARKNPRVAWMLAGVDGETIGGFG
                     LSGHGAAGFELDRADKRLGTPEHAVIIASSEGHKPEAPWVLVPEEQLTHLVTWSGEPA
                     PDLIRADMTFFETPNNGAVFSTGSITYCGSLPANGYDNPISRLTENVLRRFLEAGQAF
                     EIPDDPPA"
BASE COUNT         1241 a         2222 c         2240 g         1219 t
ORIGIN      
        1 tacaagcagc cggtggtggg tgagggtgcc ctcgtcatcg atctcgagga cggcgtcgcc
       61 ccgggggcca aacccaaggc gcgcgagaac atgcgagcgg cgctcgtcgc tcgtgaggcc
      121 aggcgcagcg agcgaatcgt gcgcatcaac gcgatgactt cgcccgagag ttcggccgac
      181 ctcggcgcac tgccaatcgc gagcgtcgat accgtgttcg tgccgaaagt ggagtcgcga
      241 gcagagatcg agcgcctcga cgccgatctt tcccgatgcg agcgcgcaca cgggtatgag
      301 ggtcatccgc caatcgggat catcgccacc atcgagacgc cgcgcgggct ctggcgcgcc
      361 ctggagatcg cagacgcctc cccccgagtg acggcgctct tcttcggctc tggcgactac
      421 acactcgcga ccggcgcctc cgtgacggag cgcgcgctcg ccggtccgcg cgccctggtg
      481 gttgcagcag ccgcggccgc cggcatcgag gccatcgatg cggcctattt cctcgacgtg
      541 aaggatcagt ctgccgtggc agctgatgcc aggatcgccc gcgagctcgg cttcaaggga
      601 aagctcgtct tccacccgag ccagatcgcg cccgtgaacg agatcatgag cccgtcggca
      661 gacgagatcg cgcgcgcccg gcgcattcgc gcggccttcg actcggctgc cgccgaaggg
      721 cgcggaactg cggtcatcga cggccagttc atcgctatcg acattgcgct gatggccgat
      781 cgcgtgctgg cgcgcgccgc agctctgggc ctcgcctgag ccgaggctgc aacctcagcc
      841 cggcggcgga ccgaacacga gcaccgcgtt gaggccgccg aatgcgaacg agttcgacat
      901 ggtgtaggtg agcttcttgg cccggcctac attcgggatg tagtcgaggt cgcactcggg
      961 atcgacctcg tcgaggttga tcgtcggcgg catccagccc tcttccatgg ccttgatgca
     1021 ggcgaccgcc tcgatgccgc cgcccgcgcc gagcgggtgg ccgtgcatcg atttcgtcga
     1081 cgacaccgca agcttgtagg cgtgcgggcc gaacacctct ttgatggcgt ttgtttcgtt
     1141 cttgtcgttg atcgtcgtcg cggtgccgtg ggcgttcaca taatcgatgg cggtcggctc
     1201 gaggccggca tcggcgaggg cgagccgcat ggcctcgcgt gggccttccg tatccgggtt
     1261 gaccatgtcc ttgctgtccg aggtcatgcc gtagccgcag atctcggcga ggatcttcgc
     1321 gccgcgccgc ttggcgtgct cgtactcctc gaggacaagg atgccggcac cctcgccgag
     1381 cacggtgccg ttgcgcttct tggcgaacgg gaacacgcct tgcggcgaga gcacgtggag
     1441 cgcctgccag gccttcatgg tgccgtagtt aatgacgctc tcggaggcgc cggcgatggc
     1501 aacatcgacg agaccctgct ggatgtagtc gcgggcaatg ccgacggcgt gcgtcgccgt
     1561 cgagcaggcg gagcaggtcg cgaaggtcgg ccccttgacg ccgaactcga tgccgacatg
     1621 ggccgaggcc gacgagccga tgatccggag cagcgtcagc ggatgcgtcg cgcgcttgtt
     1681 atggatgaag agatcgcgat aggccttctc gtaggtgacg aggccgccgg cacccgagcc
     1741 gacgatgcaa gccgctcgcg agccgttctc gaaggggacc ttgaggccag actgcttgat
     1801 cgcctcgtcg gccgcggcag ccgcgaacca ggaaaagcga tcggcgtgca ggatgatctt
     1861 gtcgcggctg acgccggtga tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnatc cttcgagtgc ggtcacgccg cactggcctt
     1981 tcctcatgcc ggaccagaac tcggtcacgt tcaggccgat cggcgtgacc acgcctagac
     2041 cagtgacgac aacgcggcgg cgtggagtgc tcatcgggtg ctcccgttca acgtcgggct
     2101 catcccaacc tctatcgaat aaacaacggt ctcagagccc aatgctcgag acgtgctcgc
     2161 acaaaaatgc gccgtcccga cgcccggctg tgccatcgtc gggcgcttgt cactcgtcat
     2221 cacgatgtta ccttggtgtg gtcgaaggcg atgtcatcat actgcggacg ggccggcata
     2281 ttggttaccg gtagaccgcg gcccgccttg gaatcatgtt gaggcggcct cgagggcggt
     2341 gtcctcgcgc gccgccggag gggcccggat caggccttcg caccagcagc ctgaccgctt
     2401 ttcgccnnnn nnnnnntgac ccggtcgacg accgagccga cagtcttgaa gtcggccgct
     2461 tcctcgttgg cgttgtaggg gatctcgatc ccgaacgcct cctcgatatc gaacacgatc
     2521 agcgcgaggt cgagcgactc gatcttgagg tccatgagcg aggtatcgcg cgaaatgtcg
     2581 tcgcgcggct ccttcatatg cttcctcagt atttcgatga tcttcttagc tacttcatcc
     2641 atgtcgctct cccagcccga gatttcacgg gcacacaggg ctgcaccgct agccctagct
     2701 agtccaaccg cgaaacaaga gcaagtttcg gcttcgcata gccgttaatg ctggaggtgt
     2761 ccagcgcttg gcggatcgag gcgaccgaag tgttgcactg ttcacgcgct gcccggatgg
     2821 ccgcacatct tgccggattt gctgtacagt tcgcattctg gcatctgcgt tcttaacccc
     2881 gcatcgaggc gattggcacc cctggttttc gaagtcgaaa tgtgttcaat ggatcacagc
     2941 agccagcaac gatgaggccg cagcgccggg ctcccggaaa gcttgccggc caatgagggt
     3001 gcggaggaag cgatgtcgaa cggcggcgcc catgccaatg gtgcggctca cggccatagc
     3061 cattatccgt ggcttgcgaa ttacccgaag gccgtgaact ggcaccagac cttcaaggcc
     3121 gagccgctgc atcggctgct cgagacgccg gcgggcaagt atccgagccg catctgcacg
     3181 aacttcctcg gcaaggcgct cacctaccgc gacatcgcct cgctcgtcga caaggccgcc
     3241 gcgggcctgc agaagctcgg cgtcgtcaag ggcacgcgcg tcggcctctt cctgccgaac
     3301 agcccgacct tcatcatcta ctacttcgcc atcctgaagg ctggcgggac ggtggtgaac
     3361 tacaaccccc tctacacgct cgatgagctc acatttcagg tgaaggacag cggcaccgag
     3421 ctgatggtga cgctcgacct cggactgctc ttcggaaagg tcgagagcct gattgccgcc
     3481 ggaacgctga agcgcgctat cgtcgcctcg ttccgcggcc tcctgccaac ggccaagtcg
     3541 gtgctgttcc gcctgttcaa gggtaaggag ctcgctccga tcgccagctc ccgggtgcgc
     3601 gaccggatca ttctcgaagc cgatgtcctc gccaatgacg gaaagccttc gccggtcgcg
     3661 atcgatgtca tgaacgacat tgccgtactg caatacacag gtggcaccac gggcacgccg
     3721 aagggtgcga tgctcaccca cgccaacgtg cacatcaacg tgcagcaggt catggcctgg
     3781 gcacccgagc tcaaggaggc cgaggagcgc gtgctcggcg tgctgccgtt cttccacgtg
     3841 ttcgcgatgt cggtcgtcat gaacttcggc atcgccaagg cgtgcgagat cgtgatcatg
     3901 ccgcgcttcg tgctcgacga cgcattgaaa ctgatcacca gcgcgaagcc gacggtgatg
     3961 cctggcgtgc cgacgctgtt caacgcgatg atgaaccacc cgaagctcaa gtccttcaac
     4021 ctctcgtcgc tcaagttctg cctgagtggc ggcgcggcac tgcccctcga cgtgaagcag
     4081 aacttcgagg cgctgacggg ctgcaagctc gtcgaaggtt acggactgtc cgaagcttct
     4141 cctgtggtca cctgcaatcc actcgatggc cccgtgaaga agaactcgat cggccagccc
     4201 ctgccggcga caatcgtctc gctgcgtgat nnnnnnnnnn cccgccgagc cgacccgtga
     4261 ggtgccgcaa ggtgacaagg gcgaggtgtg catcaagggc ccgcaggtca tgaaggggta
     4321 ctggaacaag cccgacgaga cggcgaacca gttcgtcggc gacttcctgc gcacgggcga
     4381 tgtcgcctac atggacgagg acggcttcct cttcatcgtc gatcgtatca aggacctgat
     4441 catctcgtcg ggatataacg tctatccgcg cgtcatcgag gaggcgatcc agcttcaccc
     4501 ggccgtcgag gaggtgaccg tcatcggcat cactgacaag accaggggcg aggtgccgaa
     4561 agccttcatc aagctgaaga gcggtacgcc tgcgaccgcg gacgaaatca tgaagttcct
     4621 ttccgagaaa atctcgaagc gcgagatgcc gaccgagatc gagttcaggg atgcgctgcc
     4681 gaagacgatg atcggcaagc tctccaagaa ggagctcaag gccgaggagg cgcagcgcgc
     4741 caagggttga gaatggggaa acggacaggg cggcgcctcg acgacggcga ggcaagcagc
     4801 agcagaggcg ctagatccga gaagggcagc aaaaaccgga accggccggc tgatgccttc
     4861 acgatcagtg acctcagcgc cgaattcgcg gtgacgacca ggacaatccg attctatgaa
     4921 tcggagggcc tcatatcccc gacgcggcgg ggctcgaagc gcatctactc gcgccgcgac
     4981 cgccgccgac tgatcctcat cctcagaggc aagaacctag gcttctcgct cgaggagatc
     5041 gccgagtatc tcgccctcta cgacgccgac ccttccggcg tcacgcaact aaagcacctg
     5101 atcggcaagg tggatgctgc catcgacgag ctcaatcgca agcgcaccga cctcgacagg
     5161 acgctgcgcg agttgaagga cattcaagca aaggcgcgcg aactcctggg ttagaggagc
     5221 cgctcggagc cctgattggc accaccgccg tgccgaccag agcgaaaatg gcgccaggct
     5281 caggcaggtg gatcgtccgg aatctcgaac gcctggcccg cttcaaggaa acgccggagc
     5341 acattctcgg tgaggcgcga gatcggattg tcgtagccgt tcgcgggcag cgatccgcaa
     5401 taggtgattg atccggtcga gaacaccgcg ccgttgttgg gcgtttcgaa gaaggtcatg
     5461 tcggcgcgga tgaggtctgg cgcaggctcg cccgaccagg tgacgaggtg ggtgagctgt
     5521 tcctcgggga cgagcaccca gggcgcctcg ggcttgtggc cttccgagct cgcgatgatc
     5581 acggcgtgct cgggtgtgcc gaggcgcttg tccgcgcggt cgagctcgaa gcccgccgca
     5641 ccgtgcccgg atagcccgaa gccgccgatc gtctcaccgt cgacgccagc cagcatccaa
     5701 gcgacccgag ggttcttccg tgccgccgcc tttacgcgat agtgggagcc gacgaagttg
     5761 ccttgcgccg tgaagccgac gccgacgagg ttctgcggcg ggcggccgtt gcgccgccac
     5821 atgccgccgt actcgccgtc gaaggcatta tagtattcac cgacctcggc cgcccaggcg
     5881 cgaatgccgc cctctgcgcg ccggatctcg atcgccgcgg gaagatgctt cgagcgcgca
     5941 atcttccagt agaacccgtt gccgcccatg tacatgagcc gcccgccgcc gtcgcgatag
     6001 gcggaaagcg cgtcgagcat gcgcggcgtg tgatactcgg gatgcgagcc ggtcatgacg
     6061 acgcgatagg gcctcaggac gtcgatgccc tcctcgtcga gctccaggtc ggtgatcacg
     6121 tcgtaggcga tgcccttggc ctctagccat gccgtgaggt gcgtgtcagc cggcatgtgg
     6181 cgaaggcccg agccgcggac gctctcgtcc ggataggtga tgtagcccga gcgcacggtc
     6241 agcatgggcc ttagccaaga cgcataagct atgccgctgc cgtcggtgtg atagttgtag
     6301 gtcgaaaggc cgtactcgcg gttggcgccc ggattgtgcg ggtaggcacc ccaggccttg
     6361 gcctgtgatt gccacgcctc gcgccaggct gggttggtgt tccactccgg ccgcgtgtga
     6421 ttgtggtaga cggtgtacgt gaaggtcgag acgagcacga caacgtccgc tgtgcgcttc
     6481 cccttctcgg ggacgacgaa gaacgggatg ttctcctcgg catcgccggc cttgagccgc
     6541 accgcgtagt tgcccgacct ggtgccggtt ggcacggtcc acgtgaagtc ggtggaccag
     6601 ccgcaatcat agatgtcgtc gtcgtggaaa tggatcgcgg catactcggc gggcgcatgc
     6661 cgccagcaca tctcgcggcc cgaccaggtg gcgccggcca tcgcacgcgt cggcagattg
     6721 acgagatgac cgtcgagctt ggctggcccc gtatcaacga tccgcatcga cgaaacgtcg
     6781 cggctgaaat cccatgcggc gaccagcccc gctggcacgc ggcctgcggc catgtcatcg
     6841 atctcggtat cggtcagtcc gcgggcgaag agcgccggcg cctcgatctt gccgttgaag
     6901 tggtgacgcg cctcggcgcc gccgctcgcc gcgatccgca ttaccgggcc gtcgagccgc
     6961 gggaccccgt cgaggcttgt gcgaagccca tgcgactgcc cggtcg
//
LOCUS       DAORNH010000245         6994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8008_length_6994_cov_2.463467, whole genome shotgun sequence.
ACCESSION   DAORNH010000245 DAORNH010000000
VERSION     DAORNH010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8008_length_6994_cov_2.463467"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK264_19550"
     CDS             <1..214
                     /locus_tag="PK264_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD78104.1"
                     /translation="VKRGVARGLSLKACFDEATAEMRKKYSAWPIFEHCQPFNISRAY
                     DEAQGIDTPRIWTDTRDREMWAALQG"
     gene            321..1913
                     /locus_tag="PK264_19555"
     CDS             321..1913
                     /locus_tag="PK264_19555"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD78105.1"
                     /translation="METFDYIVVGAGSAGAVLAARLSENPRTSVLLIEAGKASHAYSR
                     FPISFGLLIHNPSANWLYESDPEPGTANRQIPVPRGKLLGGSSAINGLVWVRGQPLDY
                     DTWAQMGARGWSWREVAPLFDRIEDFERGTGEGRGAGGPLHVSIVPDENPLYDALQKG
                     AVEAGFKINPDYNSEDQEGIGKTQASIRRGRRMSVAHCYLESAKTRPNLKVVTDAPVR
                     RVLLEGKRCVGIAYERWGRAFEARAGREVILSAGAVATPQLLELSGIGRPAVLKAHGL
                     EVRHASPAVGENFRDHINARIIWRVKDPRVSYNYKARGIGAVTEALRYLTTGGGFFSL
                     PSAPLVAFLKTRPEVATPDVQMHLIPYSIKDPKRRKLQDFPSMTVACYQLRPESLGSI
                     HIRSADPDAQPAIRFNFLADPIDQRTMVAGFHMMRRIVGSQAMAELAGAEVSPGSDTK
                     TDEQVLAWIRNNSQTAYHPIGTCRMGPAGPTTVVDDKLKVHGLAGLRIADASIFPTMP
                     SGNTNAPAIMVGEKAADILRAS"
     gene            complement(1929..>2937)
                     /locus_tag="PK264_19560"
     CDS             complement(1929..>2937)
                     /locus_tag="PK264_19560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD78106.1"
                     /translation="SKFGSAFIRNFAEIMRIGDDKPDLGFHPGGYLFLASTAEQEQTL
                     RENHEVQKAHGADVVLWGPGELAKAFPHLNVDDIRLSSYGRSGEGWFNNTGLMHGFRN
                     KARAQGAEYVTDEVVAIGRNGNRVTSVTLKSGAVVKAGAIVNASGPRGGRTAAMAGIA
                     LPVEPRRRTLFVFDCATTPEGSATVNQGRLPLMIDPTGVHCRPEGRYFLTGAVPPDDT
                     PPAFDDFEPGYSEFEEIIWPALVHRSKAFESIKVLRQWAGHYDYNTLDQNAIVGRHPE
                     VANFVFCNGFSGHGLQQAPGAARAVAELITYGRYRTLDLSDVGYERIAAGRPFLEKAV
                     I"
     assembly_gap    2938..3009
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3010..3130)
                     /locus_tag="PK264_19565"
                     /pseudo
     CDS             complement(3010..3130)
                     /locus_tag="PK264_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            3796..3942
                     /locus_tag="PK264_19570"
     CDS             3796..3942
                     /locus_tag="PK264_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78107.1"
                     /translation="MGKNYGFKLDGTPALALLAVIAAYFLVGRITGGTVWDRILGIRR
                     PQPR"
     gene            complement(3951..>4372)
                     /locus_tag="PK264_19575"
     CDS             complement(3951..>4372)
                     /locus_tag="PK264_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD78108.1"
                     /translation="SHSTSCQTVVTSLVVEGVFERFPKLKVVMIEGGFAWLPMLAWRL
                     DKLFERLRSEVPHLNRRPSEYIRDHVWVTTQPMEEPEDRRHLFQVIDWIGHDRLLFAS
                     DYPHWDFDDPFRAFPAGMDKSALKKICEENGKAVFRI"
     assembly_gap    4373..4388
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4389..5080)
                     /locus_tag="PK264_19580"
     CDS             complement(<4389..5080)
                     /locus_tag="PK264_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD78109.1"
                     /translation="MPPLDAPATIDVAEPRSTARNLLGTGLAYIDGDIHPAVKSMADL
                     KPYLAKQWWETLETYGIRRRHGSATVEPYPKAAPLACRRDAWPDDGRPGSDLELMQRQ
                     HLDHYGIAHGILGPLGPTGQGELNLDLSAALCSATNDWQREAFTRRDRRLKGGLVVPY
                     EDGKASAAEIERCGADLDFGQVFMLTRSQEPLGNRRYWPIYEAAERHGLPIGFHVFGT
                     SGHPTTGGGWPS"
     gene            complement(5067..6155)
                     /locus_tag="PK264_19585"
     CDS             complement(5067..6155)
                     /locus_tag="PK264_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD78110.1"
                     /translation="MPKPYAGAIDCDVHPHVASIASLSRYMSDYWRDTVALRGIESFE
                     TIAYPAKSPLTIRPELRTGAERADASAKGLAKALLDPRGVELAILNCLYAVQTIRDDN
                     LALAFSRAVNDWVRAEWLDRDPRLRASIVVPQGCPELAVEEIERIAPDQRFVQVLFVA
                     GGEHPLGKRMFWPVHAAAERHGLVIAIHAGSTYHHAVTGSGWPSYYLEDYAAQAAIFH
                     AQLGSLIAEGVFVKFPKLKAVLVESGVTWLPAYLWRLSKFWRGVRNEVPWIDRPPIEI
                     VRDHIRLTAQPFDGPSDAASVQRIMDLIGSDDLLLFASDFPHWHDDGGDLLPAGLTPA
                     LRRKIMIDNPRATYPRLAANGGGADAAP"
     gene            6373..>6994
                     /locus_tag="PK264_19590"
     CDS             6373..>6994
                     /locus_tag="PK264_19590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD78111.1"
                     /translation="MRIGVAAFTGALLIAVSLPAYADNWSECASEDAETSIRACTRII
                     AVRNQVKDKTRLALAYYHRGTSYYRRNDCTAGIADLSESLRLNASDHRAFNNRGSCYL
                     KTNDPTRARGDFDEALRLDPTYVLGYLNRGIASEQLGDFTRARADFTSGLAIPARRDI
                     DTNAHAEIRRRLAALGAPGTGPATKPASPPPTAAATPPPTGTSGVAS"
BASE COUNT         1170 a         2267 c         2332 g         1137 t
ORIGIN      
        1 ggtcaagcgc ggcgtcgcgc gtggcctctc gctcaaggcc tgcttcgacg aggcgacggc
       61 cgagatgcga aagaagtact cggcctggcc gatcttcgag cactgccagc cgttcaacat
      121 ctcgcgcgcc tacgacgagg cgcagggtat cgatacgccg cgcatctgga ccgacacgcg
      181 cgatcgcgag atgtgggcgg cgctgcaggg ttgaggctgc ttttctgaca tgccaggatc
      241 gccacgaggg cgctcgacca tgcgatcgag cgccttcatc atggttgcgt caggcttggc
      301 gcgggcgaac tgggggcagc atggaaacgt tcgactatat cgtcgtcggt gcgggctcgg
      361 ctggtgcggt gctggcggcg cggctctccg agaacccgag gacgagcgtc ctgctcatcg
      421 aagccggcaa ggcgagccac gcctactcgc gctttccgat cagtttcggg ctgctcatcc
      481 acaatccctc agccaactgg ctctatgaat cggaccccga gcccggcacg gcgaaccgcc
      541 agatcccggt gccgcgcggc aagctgctcg gcggctcgag cgccatcaac ggcctcgtct
      601 gggtgcgcgg ccaaccactc gactacgata cctgggcaca gatgggtgcc cgcggctgga
      661 gctggcgcga ggttgcaccc ctcttcgatc gcatcgagga cttcgagcgc ggcaccggcg
      721 agggtcgcgg tgccggcggg cccctgcacg tctcgatcgt gccggacgag aacccgctct
      781 acgacgcgct ccagaagggt gcggtcgagg cgggtttcaa gatcaatccc gactacaaca
      841 gtgaggacca ggagggcatc ggcaagacgc aggcgagcat ccggcgcggg cggcgcatga
      901 gcgtcgcgca ctgctacctc gagagcgcga agacgcggcc gaacctcaag gtcgtcaccg
      961 atgcgccagt gcgccgcgtg ctgctcgaag ggaagcgatg cgtcggcatc gcctacgagc
     1021 gctggggacg ggcattcgag gcgagggccg ggcgcgaggt gatcctgtcg gcgggggcgg
     1081 tcgcgacgcc gcaactgctc gagctttcgg ggatcggacg gcccgcggtg ctgaaggcgc
     1141 acggtctcga ggtgcggcac gcgagcccgg cggtcggcga gaacttccgc gaccacatca
     1201 atgcccgcat catctggcgc gtgaaggacc cgcgcgtctc ctacaactac aaggcgcgcg
     1261 ggatcggcgc ggtgacagaa gctctgcgct acctgacgac gggcggcggc tttttcagcc
     1321 tgccgtcggc gccgctcgtc gccttcctga agacgcgccc cgaggtcgcg acgcccgatg
     1381 tccagatgca cctgatcccc tattctatca aggatccgaa gcgacggaag ctccaggatt
     1441 tcccgtcgat gacggtcgcc tgctaccagc tgcgccccga gagccttggc tcgatccata
     1501 tccgctcggc tgacccggac gcgcagccgg cgatccgctt caactttctc gccgacccga
     1561 tcgaccagcg cacgatggtg gcgggcttcc acatgatgcg ccgcatcgtc ggctcgcagg
     1621 cgatggcgga gcttgccggc gccgaggtct cgcccggctc cgacacgaag acggacgagc
     1681 aggtgctcgc ctggatccgc aacaactcac agacggccta tcatccgatc ggcacctgcc
     1741 gcatggggcc tgcgggaccg acgaccgtcg tcgacgacaa gctcaaagtg cacgggctcg
     1801 caggcctcag aatcgccgac gcgtcgatct tcccgaccat gccgtccggc aataccaatg
     1861 ccccggcgat catggtcggc gagaaggccg ccgacatcct gcgcgcgagc tgaggtgcgg
     1921 agcggccgtc agatgacggc tttctcgagg aacgggcggc cggcggcgat gcgctcgtag
     1981 ccgacgtcgg acaggtcgag agtgcgatag cggccgtagg tgatcagctc tgcgacggct
     2041 ctcgccgcgc ccggtgcctg ctgcaggcca tggccggaga atccattgca gaagacgaag
     2101 ttcgcgacct ccggatgacg cccaacgatc gcgttctggt ccagcgtatt gtagtcgtag
     2161 tggcccgccc actgccgtag taccttgatg ctctcgaacg ccttggagcg atgcacgagc
     2221 gccggccaga tgatctcctc gaactcgctg tagccgggct cgaagtcgtc gaatgcgggc
     2281 ggggtgtcat ccgggggcac ggcgcccgtg aggaagtagc gcccctccgg ccggcaatgc
     2341 acgcccgtcg gatcgatcat cagcggaaga cggccctgat tgacggtcgc tgatccttcc
     2401 ggcgtcgtcg cgcaatcgaa gacgaaaagc gtgcgccggc gcggctcgac cggaagcgcg
     2461 atccctgcca tggccgcggt acgtccgccg cgcgggcccg acgcattgac gatcgcgccg
     2521 gccttgacga ccgcaccgga ctttagcgtc accgaggtca cgcggtttcc gttccgaccg
     2581 atcgcaacga cctcgtcggt cacgtactcg gcgccctgcg cgcgggcctt gttgcggaag
     2641 ccgtgcatga ggccggtgtt gttgaaccag ccctcgccag agcggccata ggacgagagg
     2701 cggatgtcat cgacgttcag gtgggggaat gccttcgcaa gctcgcccgg tccccacagc
     2761 accacgtcgg cgccgtgcgc cttctgcacc tcgtggttct cgcgcagcgt ctgctcctgc
     2821 tcagccgtgc tcgcgaggaa cagatagccg cccgggtgaa atccgagatc gggcttgtcg
     2881 tcgccgatcc gcatgatctc ggcgaagttg cggatgaacg cgctgccgaa cttgctgnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnna ggggtcacgc tcgatgacca ggatcgagcc ctttaagtcg ggactggcgc
     3061 tcaggaaata ggcgacgccc gagccgatca ctgcgccgcc gacgatgacg acgtcatagc
     3121 tgtccttcac ggtgacatct cctcattgga gggccaggat tgtggcaccg ggccctcgtg
     3181 caggctaccg ccggcgcaca cgaagccttc gccgaaaagc gatcttgggt ttcctgtctt
     3241 ccatggatga taggtcgatt cgggtctcga tcggtcgagg ctgctcaccg gagagacgat
     3301 cgatagttgc tggacggcgt aacaggcagc gttggggacg tttcatccgc agggctttgc
     3361 ttggcggcgt cccgggacgc ttcctattgt gacgagagct ggtggtggaa gccgagccga
     3421 gtgatagtgc gtctagcttg gttacttgat ggccggtatc ataccgccac ggaagcacca
     3481 gtcggcgccg cgatgggtgg acgggcgggc gccgatcggt gcatcggtct ctagggcgcg
     3541 cctagcgccc tggttcagct ggccggggac gtgctccaat ccaagcattg cgatcaggct
     3601 gacattcgag gggcacgccc gagtgggcag cccgacacgg caatgaccga aaggcacggg
     3661 gtggcagatg tcatgaccac gtcaatcacg cctgcaccgg tttggaagcg catactcgca
     3721 acgtgactcg atgccttcat ggtgttcatg gttgccggat ttgcgatcgg ctaggccacc
     3781 ggcaatgccg aggtgatggg caagaattac ggcttcaagc tcgacggcac acccgcgctg
     3841 gcgctccttg cggtgatcgc cgcctatttc ctcgtcggcc gcatcacggg cggcacggtc
     3901 tgggatcgta tcctaggcat ccgccgccca cagccccgtt gaggcccgcc tcagatccgg
     3961 aacacggcct tgccgttctc ctcgcagatc ttcttgaggg cgctcttgtc catgccggcg
     4021 gggaaggcgc ggaatgggtc gtcgaagtcc cagtgggggt agtcgctcgc gaacagcagg
     4081 cggtcgtggc cgatccagtc gatcacctgg aagagatggc ggcggtcctc cggctcctcc
     4141 atgggctgcg tcgtcaccca gacgtggtcg cggatgtatt cggacgggcg ccgattcagg
     4201 tgtggaacct cgctacggag ccgctcgaag agcttgtcga ggcgccaggc gagcatcggc
     4261 agccaggcaa agccgccttc gatcatgacg accttcagct tcgggaagcg ctcgaacacc
     4321 ccctcgacaa ctaggctcgt caccaccgtc tggcaggagg tcgagtggct ggnnnnnnnn
     4381 nnnnnnnnta ggagggccag ccgccgccgg tggtcggatg gccggaggtg ccgaacacgt
     4441 ggaagccgat cggcaggccg tggcgctccg ctgcctcgta gatcggccag tagcggcggt
     4501 tgccgagcgg ctcctgcgaa cgcgtcaaca tgaacacctg cccgaaatcg aggtcggcgc
     4561 cgcagcgctc gatctcggcg gcggaggcct tgccgtcctc gtagggcacc acgagcccac
     4621 ccttgaggcg cctgtcgcgc cgcgtaaagg cctcgcgctg ccaatcgttg gtggccgagc
     4681 agagtgctgc ggaaagatcg agattgagct cgccctgtcc agtcggaccg agggggccaa
     4741 ggatgccgtg agcgatgccg tagtgatcga gatgctggcg ctgcatcagc tcgagatcgg
     4801 agccgggccg gccgtcgtct ggccaggcat cgcgccggca ggcaaggggc gcggccttcg
     4861 gatagggctc gacagttgcg ctgccgtgcc ggcggcggat gccgtaggtc tcgagcgtct
     4921 cccaccactg ctttgcgaga tacggtttca ggtcggccat gctcttcacg gcagggtgga
     4981 tgtcgccgtc gatgtaggcg agcccggtgc cgaggagatt cctcgccgtg gagcgcggct
     5041 cggcgacgtc gatggtcgca ggcgcatcaa ggggcggcat cggcacctcc accatttgcc
     5101 gcgaggcgag ggtaggtcgc tcgcgggttg tcgatcatga tcttgcggcg caacgcgggc
     5161 gtgaggcccg cgggaagcaa atcgcccccg tcatcatgcc agtggggaaa gtcgctggca
     5221 aacaacagga ggtcgtccga gccgatgagg tccatgatcc gctgcacgct tgccgcatcg
     5281 ctgggtccgt cgaagggctg cgcggtcaag cggatgtggt cgcgcacgat ctcgatcggc
     5341 gggcggtcga tccagggcac ctcgttgcgc acgccgcgcc agaacttgga gagccgccag
     5401 aggtaggccg gcagccacgt gacgcccgat tcgaccagca cggccttcag cttcgggaac
     5461 ttcacgaaca cgccctcggc gatcaggctg ccgagctggg catggaagat cgccgcctgg
     5521 gcggcgtagt cctcgaggta gtaggagggc cagccggagc ctgtgacggc gtggtggtag
     5581 gtcgagcccg cgtgaatggc gatcaccagg ccgtggcgct ccgctgccgc atgcaccggc
     5641 cagaacatgc gtttgccgag cggatgctcg ccgcccgcga caaagagaac ctgcacgaag
     5701 cgctggtccg gggcgatccg ctcgatttcc tcgaccgcga gctccgggca gccctgcggc
     5761 accacgatcg aagcccgcag gcgcggatcg cggtcgagcc actcggcgcg cacccaatcg
     5821 ttgacggcgc gcgagaaggc gagcgccagg ttgtcgtcgc ggatggtctg gaccgcatag
     5881 aggcaattga ggatcgcaag ctcgacgccg cgcggatcga gcagcgcctt ggcgagaccc
     5941 ttggccgagg catcggcccg ctccgcgcca gtgcgcagct ccggccggat ggtgagtggc
     6001 gacttcgccg ggtaggcaat tgtttcgaac gattcgatgc cgcgcagggc cacagtatcg
     6061 cgccaatagt cgctcatgta gcgcgacagc gacgcgatgc tcgcgacgtg cgggtggaca
     6121 tcgcagtcga tggctccggc atacggcttt ggcatcgcgt cctcgtgtgg cgtcggaggc
     6181 aggatactcc cgatcggccg cggatggcag aggcggcgtc agagcctcag cgcatgcctc
     6241 ccctaccgca attcggcctc tgtcgctccc aaggaaccaa tcgcggcaga gggaccgttg
     6301 gccgggacgc gtggtcctcc tatactcgcg acgatttcag ccctcgggcc cgagcggcta
     6361 ttggaggacg acatgcggat cggcgttgca gccttcaccg gcgcactact cattgccgtc
     6421 tcactgcccg cctacgccga caactggtcg gaatgcgcct cagaggacgc cgagaccagc
     6481 atccgcgcct gcacccgcat catcgcggtt cgcaaccagg tcaaggacaa gacgcgactg
     6541 gcgctcgcct attaccaccg tggcacgtcc tactaccggc gcaacgactg cacggccggt
     6601 attgcggacc tttccgagtc cctccgcctc aatgccagcg accaccgcgc cttcaacaat
     6661 cgcggcagct gctacctgaa aacgaacgat ccgacccgcg cgcgcggcga cttcgacgag
     6721 gccttgcgtc tcgatccgac ctatgtgctc ggctatctca atcgcggcat cgccagcgaa
     6781 cagctcggcg atttcacgcg tgcgcgggcc gacttcacct ccggactcgc cattcccgcg
     6841 cgccgcgaca tcgataccaa tgcacatgcc gagatccgcc ggcgattggc ggcgctcggc
     6901 gctcctggta ccggtccggc caccaagccg gcgtcgccac cgccgactgc cgccgcgacg
     6961 ccaccaccga cgggcacatc gggcgtcgca tccg
//
LOCUS       DAORNH010000246         6966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8046_length_6966_cov_2.830271, whole genome shotgun sequence.
ACCESSION   DAORNH010000246 DAORNH010000000
VERSION     DAORNH010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8046_length_6966_cov_2.830271"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK264_19595"
     CDS             <1..479
                     /locus_tag="PK264_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRD78112.1"
                     /translation="SGLPFDDPSGDRLRAWLGLDRATFYDEARIAFLPMGLCFPGHDA
                     KGGDLPPRPECAPRWRASLLAALPAIEVALLIGLYAQRWHLPRELTREGLTVTVARWR
                     EIMADRVPATPLFIPLPHPSWRNSGWLKRHRWFEAELVPELQDVVARTLNQRRDMP"
     gene            539..976
                     /locus_tag="PK264_19600"
     CDS             539..976
                     /locus_tag="PK264_19600"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HRD78113.1"
                     /translation="MPTTCFRRAALLGAISLAAIASPLVAQPATPPTKIDCETAKTTP
                     DIAWCAGEELKAADAELNKAYQAALTYIAKADHLNVNQRRDWRRALQEAQRHWLAFRK
                     KDCGEVTGWEWYGGTGMGTASLGCEIAKTKTRTEELKARYGKQ"
     gene            complement(987..1337)
                     /gene="cutA"
                     /locus_tag="PK264_19605"
     CDS             complement(987..1337)
                     /gene="cutA"
                     /locus_tag="PK264_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HRD78114.1"
                     /translation="MPADAPEDRAVLMYVTCPTAGEAERIGGGLVDRRLAACVNILPE
                     MTAIFQWQGERQTEREAVMIVKTRAALADAVTAAIRATHPYTNPAIVVLEIAGGSPEF
                     LAWILAETRSPASG"
     gene            1428..2234
                     /locus_tag="PK264_19610"
     CDS             1428..2234
                     /locus_tag="PK264_19610"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HRD78115.1"
                     /translation="MSEASSSTTAAGRGGGKRLAFVASDVQEAREAFDTLVARYGNVP
                     PNDADAIVALGGDGLMLQTLHRHLDDRIPIYGMNRGSVGFLMNEYREDDLIERIARAE
                     VSRIHPLRMIVTDAAGRRHEGLAINEVSLWRETHQAAKLRISVDGTVRLEELICDGIL
                     VATPAGSTAYNLSAHGPILPINAQLMALTPISPFRPRRWRGALLPSVARVMIEVLEAA
                     KRPVSASADNTETRSVVQVEVAEARHIDLLMMFDPGRSLDERILSEQFRF"
     gene            2329..2709
                     /gene="crcB"
                     /locus_tag="PK264_19615"
     CDS             2329..2709
                     /gene="crcB"
                     /locus_tag="PK264_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRD78116.1"
                     /translation="MTLFLLASAGGAIGAGARYLVGIAAAAWVGFAFPWGTLVVNVVG
                     SFLMGIVVETIALRCGGSVELRTFLATGILGGFTTFSAFSLEVVALAERKQPGLAIAY
                     VAASVLLSILALIAGTALTRSACS"
     gene            2706..3689
                     /locus_tag="PK264_19620"
     CDS             2706..3689
                     /locus_tag="PK264_19620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRD78117.1"
                     /translation="MSVETIRVTNRDDGLRVDRWFKTHYPHVTHGFLQKLLRTGQVRV
                     NSKRAKADLRLAAGQEIRVPHTVREAAKPKPSLKPPPGLSKADRTFIEHMILYEDDHV
                     LVLNKPFGIAVQGGTGTTRHIDGLLAGMEDRFGDRPRLVHRLDRDTTGVLLVAKHRDG
                     AAKLGRIFQTRSAAKTYWAIVKGVPRPPQGKVEAALVKASGPEGDRVRKALPGEQEAA
                     MHATTHYSVIDRAASKAAWVSLKPVTGRQHQLRAHMALIGHPIVGDNKYEGGLELPAE
                     NMDAKLHLHARRLILPHPSGEGKIDVTAPLSDHMRRSFELLGFDPERFEEG"
     gene            complement(3709..4098)
                     /locus_tag="PK264_19625"
     CDS             complement(3709..4098)
                     /locus_tag="PK264_19625"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD78118.1"
                     /translation="MPVQITGLYAGLLAIVGLVLVTLVGQQRGRTNISLGDKGDRELI
                     VANRRHMNFIENVPFALLLIGLVELNGGAKWWVHGLGAALLAARIIHPFGLDPDRMML
                     WPRIIGAGATIIVQLLAALTLLWMFVR"
     gene            complement(4123..4842)
                     /locus_tag="PK264_19630"
     CDS             complement(4123..4842)
                     /locus_tag="PK264_19630"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRD78119.1"
                     /translation="MTSFAVILKRAEERKGGAKALAKLLPPRPDHKALARLGDDRILA
                     EMTKRIFCSGFAWKVIDAKWAGFEAAFHNFDPKRLVFAPDEFWDGLTKDTRIVRNGAK
                     IMSVRENARFIREIAEEHGSFARMLAKWPSSDEIGLLELLARRGNRLGGNTGQMTLRF
                     LGWDGFVTSRDVVACLRDAGVEVGEEVKSKRDLKRVQDQFNAWAAETGRPYVHLSRIC
                     AMSIGENYEASRLSSLEGGET"
     gene            complement(4879..5427)
                     /locus_tag="PK264_19635"
     CDS             complement(4879..5427)
                     /locus_tag="PK264_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRD78120.1"
                     /translation="MASMTLTLLRHAKSSWDNPHIDDFDRPLAPRGRAAAPLMGEFMA
                     RQGIAPDLVICSEAQRARETLALVLAKIGAPREIRHQRQLYLAPPNRMTRIILTAWRG
                     PEADRSRHIMLVGHNPGLHALAVELAEMGDAGLRADLAQKFPTTGLVVIEFGGDDPIH
                     DLRGGRLRHFMTPRRLAGDPAP"
     gene            complement(5445..>6966)
                     /locus_tag="PK264_19640"
     CDS             complement(5445..>6966)
                     /locus_tag="PK264_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRD78121.1"
                     /translation="GMRIAASRKEVAEGFALARSEAKSSFGDDRVFVEKFIVDPRHIE
                     IQVLGDKHGNVIYLGERECSIQRRNQKVIEEAPSPLLDEKTRRAMGEQAVALAKAVGY
                     DSAGTVEFVAGQDRSFFFLEMNTRLQVEHPVTELVTGLDLVELMIRSAAGEKLPLKQA
                     DVKLKGWAIESRIYAEDPYRNFLPSIGRLSTYRPPAEGVSDGKTIRNDTGVYEGGEIS
                     MFYDPMIAKLCTHGPTRDSAIDLMAEALDAFAIDGIQHNIPFLTALMQSPRWRSGKLS
                     TGFIREEYPSGFKPRQPDPMERERVAAVAAHIDHLGNSRRRQISQQITGPSVRFAKQR
                     IVDVGGTKVAMEVCGELGEPTYVTILDDHGKPRETIEVVSSWWPGESVWQGKVGGKPV
                     SVLVKPTLNGNILAHRGLAAVTYVYTQREAELAGLMPTKSAADTSKLLLCPMPGLIKA
                     IHVKEGAEVKAGDSLAVVEAMKMENILKAERDAVVKAVKCKAGDSLAVDAVILEFA"
BASE COUNT         1126 a         2420 c         2282 g         1138 t
ORIGIN      
        1 cgtcggggct gccattcgac gacccgtccg gcgaccggct ccgcgcctgg ctcgggctcg
       61 accgggcgac cttctacgac gaggcgcgta tcgccttcct gccgatggga ctctgcttcc
      121 ctggccacga cgcgaagggc ggtgacctgc cgccgcgccc tgagtgcgcg ccacgctggc
      181 gggcgtcgct gctggcggcg ctcccggcga tcgaggtcgc gctcctcatc ggcctgtacg
      241 cacagcgctg gcacctccca cgcgaattga cgcgcgaagg cctcaccgta accgtcgcgc
      301 gttggcgcga gatcatggcc gaccgcgtgc cagcgacgcc gctcttcatt cccctgccgc
      361 atccctcgtg gcgcaactcc ggctggctga agcgccaccg ctggttcgag gccgagctcg
      421 tcccggaact ccaggatgtc gtcgcgcgga cccttaacca gaggcgggac atgccttgaa
      481 taagcgcgca ggccgtggtc caactcgccc gaacgttcct cgagccaccg ggccacacat
      541 gccaacgaca tgctttcgcc gcgccgcgct cctcggagcg atcagccttg ccgccatcgc
      601 ctcgcccctt gtggcccagc cggccacgcc gccaacgaag attgattgcg agacggcgaa
      661 aaccacgccc gacatcgcct ggtgtgccgg cgaggagctg aaggctgccg atgccgagct
      721 caacaaggcc taccaggcgg cgctcaccta catcgcgaag gccgaccacc tgaatgtcaa
      781 ccagcgtcgc gactggcgcc gcgcgctgca ggaggcgcag cgccattggc tcgccttccg
      841 caagaaggac tgcggcgagg tgacgggctg ggaatggtac ggcggcaccg gcatgggcac
      901 cgcgtcgctc ggctgcgaga tcgccaagac gaagacgcgc acggaagagc tgaaggcgcg
      961 ctacggcaag cagtagcttt cgcgcttcag cctgacgcgg gcgatctcgt ctcggccagg
     1021 atccaggcga ggaattccgg cgatccgccg gcgatctcga gcacgacgat ggccgggttg
     1081 gtgtaagggt gcgtcgctcg gatggccgcc gtcacggcgt ccgcgagggc cgcgcgggtc
     1141 ttcacgatca tgacggcctc gcgctccgtc tgccgctcgc cctgccactg gaagatcgct
     1201 gtcatctcgg gaaggatgtt gacgcaggcc gcgagccgcc ggtcgacgag gccgccgccg
     1261 atgcgctccg cctcgccggc agtcgggcac gtcacataca tcaagacggc gcgatcctct
     1321 ggagcatctg cgggcatcgg cgtatcctac gggtgacggc ggcgcgagcc gcgactgtag
     1381 cgccggcctt tcggtcctgc acctgctcga actaggaacg aacgcgaatg agtgaagctt
     1441 cgagcagtac gacggctgca ggccgcggag gcggcaagcg cctcgcgttc gtcgcgagcg
     1501 acgtgcagga ggcgcgtgaa gcctttgaca cgctcgtcgc gcgctatggc aacgtcccgc
     1561 ccaatgacgc tgacgccatc gttgcgctcg gcggcgacgg cctcatgctg cagacgctgc
     1621 atcgccacct cgatgaccgg atcccgatct acggcatgaa ccgcggctcg gtcggcttcc
     1681 tcatgaacga gtaccgcgag gacgacctca tcgagcggat cgcgcgcgcc gaggtgagcc
     1741 gcatccaccc gctgcgcatg atcgtcaccg atgctgccgg ccgacgccac gagggcctcg
     1801 ccatcaacga ggtctcgctc tggcgcgaga cacatcaggc ggcgaagctg cgcatctccg
     1861 tcgacggcac cgtccggctc gaggaactca tctgcgacgg catcctggtt gcgacacccg
     1921 cgggatcgac cgcctacaat ctctccgccc acggccccat cctgccgatc aacgcgcagc
     1981 tcatggcgct gacgccgatc agcccgttcc gcccacgtcg ctggcgcggc gcgctgctgc
     2041 cgagcgtcgc ccgcgtcatg atcgaggtgc tcgaagccgc caagcggccg gtgagtgcct
     2101 cggccgacaa taccgagacg cgctcggtcg tgcaggtcga ggttgcggag gctcgccaca
     2161 tcgacctgct catgatgttc gatcccggcc gtagcctcga cgagcgcatc ctctccgagc
     2221 agttccgctt ctgaagcctg cttgccctca ccgtcggctc cgctatagcg atggggtccc
     2281 gaggctgcgc gggacagccg cgacacatcg agggccggat aggaattcat gacactcttc
     2341 ttgcttgcat cggccggcgg ggcgatcgga gcgggcgcgc gctatctcgt cgggatcgcg
     2401 gcggcggcct gggttggttt cgcattcccg tggggcacgc tcgtcgtcaa cgtcgtcggc
     2461 tcgttcctga tgggtatcgt cgtcgagacg atcgcactgc gctgcggcgg ctcggtcgag
     2521 ctgcgcactt tcctggcaac cggcatcctc ggcggcttca ccacgttctc cgcattctca
     2581 ctcgaggtgg tcgcactcgc cgagcgaaag cagcccggcc tcgcgattgc ctatgtcgcc
     2641 gcatcggtgc tgctgtcgat acttgcgctg atcgccggaa ccgccctcac ccggagtgcc
     2701 tgctcatgag cgtcgagacg atccgagtga cgaaccgcga cgacggtctc cgcgtcgatc
     2761 gctggttcaa gacgcattac ccgcacgtca cgcatggctt tctgcagaag ttgctccgca
     2821 cgggccaggt gcgggtgaat tcgaagcgcg cgaaggccga tctgcgcctt gcggcgggcc
     2881 aggagatccg cgtgccgcat acggtgcgcg aggcggccaa gccgaagccc tcgctgaaac
     2941 cgccgccggg gctctcgaag gccgatcgca ccttcatcga gcacatgatc ctctacgagg
     3001 acgatcatgt gctggtcctc aacaagccgt tcggcatcgc cgtgcagggc ggcaccggca
     3061 cgacgcgcca catcgacggg ctgctcgccg gcatggagga tcgcttcggc gaccggccgc
     3121 gcctcgtgca ccgactcgac cgcgatacga cgggcgtgct gctcgttgcc aagcaccgcg
     3181 acggcgccgc caaactcggc cgcatcttcc agacacgctc cgccgccaag acctactggg
     3241 cgatcgtgaa gggcgtaccg aggccgccgc aggggaaggt cgaggccgcc ctcgtcaagg
     3301 cctcgggtcc ggagggtgat cgcgtgcgca aggcgctgcc cggcgagcag gaagcggcga
     3361 tgcacgcgac gacgcactac tcggtgatcg accgggccgc gagcaaggcc gcctgggtgt
     3421 cgctgaagcc cgtcacgggc cgccagcatc agctgcgcgc gcacatggcg ctgatcgggc
     3481 acccgatcgt cggcgacaac aagtacgaag gcggcctcga gctgccggcg gagaacatgg
     3541 acgcgaaact ccacctccac gcccgccgcc tgatcctacc ccacccgtcc ggcgaaggaa
     3601 aaatcgacgt caccgcgccg ctctccgacc acatgcgccg ctccttcgag ctcctcggct
     3661 tcgaccccga gcgcttcgag gaggggtgat ccgggtcagc tccgcctgct acctgacgaa
     3721 catccacagc agcgtcagcg cggcgagcag ctgcacgata atggtggcgc cggcgccgat
     3781 gatgcgtggc cagagcatca tgcgatcggg gtcgaggccg aaggggtgga tgatccgcgc
     3841 cgcgagaagc gccgcgccaa gcccgtgcac ccaccatttg gcgccgccgt tgagctcgac
     3901 gaggccgatc agcagaagcg cgaacggcac gttctcgatg aagttcatgt gtcggcgatt
     3961 ggcaacgatc agctcgcggt cgcccttgtc gccgagcgag atgttcgtgc gtccgcgctg
     4021 ctggccgacc aacgtgacga gcacgagccc gacgatcgcc agcaggcccg cataaagccc
     4081 cgttatctgt accggcatcg cgacactccc ccagatccac cgtcatgtct cgccgccctc
     4141 gagcgaggac agcctcgagg cctcgtagtt ctcgccgatc gacatcgcac aaatgcgcga
     4201 gagatgcacg taggggcgcc cggtctccgc tgcccaggca ttgaactggt cctgcacgcg
     4261 cttcaggtcg cgcttcgatt tcacctcctc accgacctcg acaccggcgt cgcggaggca
     4321 ggccacgaca tcccgcgagg tgacgaagcc gtcccaaccg aggaagcgca gcgtcatctg
     4381 cccggtattg ccgccgagcc ggttgccgcg cctggcgagg agctcgagga gcccgatctc
     4441 gtccgacgac ggccatttgg cgagcatgcg ggcgaagctg ccgtgctcct ctgcgatctc
     4501 gcggatgaag cgtgcgttct cgcgcacgga catgattttc gcgccattgc ggacgatgcg
     4561 cgtgtccttg gtcagcccat cccagaactc gtccggcgcg aagacgaggc gcttcggatc
     4621 gaagttatgg aaggccgcct cgaagcccgc ccacttggca tcgatcacct tccaggcgaa
     4681 gcctgagcag aagatgcgct tcgtcatctc ggcgaggatg cggtcgtcgc cgagtctggc
     4741 gagcgccttg tgatcgggcc tcggcggcag cagcttggcg agcgccttcg ccccaccctt
     4801 gcgctcctct gcccgcttca ggatcactgc gaacgacgtc atgggcgtgc tccttcggtg
     4861 gcggcgtcgc tccggccgtc acggcgccgg atcgcctgcg aggcggcgcg gcgtcatgaa
     4921 atgccgaaga cggccgccgc gcaagtcgtg gatcgggtcg tcgccgccga actcgatcac
     4981 cacgaggccc gtcgtcggga acttctgcgc gaggtccgcc ctaaggccgg cgtcccccat
     5041 ttccgcaagc tcaaccgcga gcgcatgcag gcccgggttg tgcccgacga gcatgatgtg
     5101 gcgtgaacga tcggcctcgg gcccgcgcca tgccgtgagg atgatccgcg tcatccggtt
     5161 cggcggggcg agatagagct gacgctgatg gcggatctcg cgcggcgcgc cgatcttcgc
     5221 gaggacgagc gccagcgtct cgcgcgcccg ctgagcttcc gagcagatca cgagatcggg
     5281 cgcgatgccc tgccgcgcca tgaactcgcc catgaggggc gccgcggcac ggccgcgggg
     5341 agcaagcggc cggtcgaagt cgtcgatatg aggattgtcc cagctcgact tcgcatgccg
     5401 caagagcgtc agcgtcatgc tcgccatggc gtctccgttc cggctcaggc gaactcgagg
     5461 atcaccgcgt cgacggcaag gctgtcgccg gccttgcatt tcacggcctt cacgaccgca
     5521 tcgcgctcgg ccttaaggat attctccatc ttcatggcct cgacgacggc aaggctgtca
     5581 ccggccttca cctccgcccc ttccttgacg tggatcgcct tgatgagacc gggcatcggg
     5641 caaagcagga gcttcgaggt atcggccgcg ctcttcgtcg gcatcaaacc agcgagctcc
     5701 gcctcgcgct gcgtgtacac gtaggtgacg gccgcgaggc cgcgatgcgc gaggatattg
     5761 ccgttgagcg tcggcttcac cagaaccgag acaggcttgc cgccgacctt cccttgccag
     5821 accgactcgc cgggccacca cgacgagaca acctcgatcg tctctcgcgg cttgccgtgg
     5881 tcgtcgagga tcgtgacata ggtcggctcg ccgagctcgc cgcaaacctc catggcgacc
     5941 ttcgtgccgc cgacatcgac gatgcgctgc ttcgcgaagc ggacggaagg gcccgtgatc
     6001 tgctggctga tctggcggcg gcgactgttg ccgagatgat cgatatgcgc ggcgaccgcg
     6061 gcgacgcgct cgcgctccat cgggtccggc tggcgcggtt tgaacccgct cggatactcc
     6121 tcgcggatga aacccgtcga gagcttgccc gagcgccagc gcgggctctg catgagtgcg
     6181 gtgagaaacg ggatgttgtg ctggatgccg tcgatcgcga acgcgtcgag cgcctcggcc
     6241 atcaggtcga tcgcgctgtc gcgtgtcgga ccatgcgtgc agagcttggc gatcatcggg
     6301 tcgtagaaca tcgagatttc gccgccctcg tagacgcccg tatcgttgcg gatcgtcttg
     6361 ccgtcgctga ccccctcggc gggtggacga taggtcgaca ggcgcccgat cgaggggagg
     6421 aagttccgat acggatcctc ggcgtagatg cggctctcga tggcccagcc cttgagcttc
     6481 acgtccgcct gcttgagcgg cagcttctcg ccggcggccg atcggatcat cagctccacg
     6541 agatcgagcc cggtgacgag ctcggtcacc gggtgctcga cctggaggcg ggtgttcatc
     6601 tcgaggaaga agaacgagcg gtcctggccc gcgacgaact caacggtgcc cgcgctgtcg
     6661 tagccgacgg ccttggctag cgccaccgcc tgctcgccca tcgcccgccg cgtcttctcg
     6721 tcgagcagcg gcgagggcgc ctcctcgatg accttctggt tgcgccgctg gatcgagcac
     6781 tcgcgctcgc cgagatagat gacgttgccg tgcttgtcgc cgagcacctg gatttcgatg
     6841 tggcgcggat cgacgatgaa cttctcgacg aacacgcgat cgtcgccgaa cgaggatttt
     6901 gcctccgagc gggcgagcgc gaagccctcg gcgacctcct tgcggctcgc cgcgatgcgc
     6961 ataccc
//
LOCUS       DAORNH010000247         6915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8124_length_6915_cov_2.650292, whole genome shotgun sequence.
ACCESSION   DAORNH010000247 DAORNH010000000
VERSION     DAORNH010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6915
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8124_length_6915_cov_2.650292"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..>932)
                     /locus_tag="PK264_19645"
     CDS             complement(129..>932)
                     /locus_tag="PK264_19645"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRD78122.1"
                     /translation="HRNRCSLRLLGARVSLEWPLVAALGAASFVGALVQAATGFGYAI
                     LAAPVFLWAMNSTRAMAVLVGLHLVQSAMMVPRLLQHVDRRRLGLLAAGALVGCPLGL
                     AILLRLDLSVLKIAVGIVILLAAALLIAREIGLIGARPRSEASPRPALTGATGIASGL
                     LTALLVMPGPPLMLLAAADRLPRDVTRALSLTFFALCYAFVTALYTASGTFDREAALV
                     TLLLAPTAALGTLIGARAANALGESRFRAAVLTLLVLSGAGALAAGLRG"
     assembly_gap    933..948
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<949..1239)
                     /locus_tag="PK264_19650"
     CDS             complement(<949..1239)
                     /locus_tag="PK264_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRD78123.1"
                     /translation="MTGLAGIWPYLVPALLLIASNVFMTLAWYGHLSYKSQPLFVVIL
                     VSWGIAFIEYCLAVPANRFGHGLYSAAELKTMQEVITLLVFAVFSVVWLKEAI"
     gene            complement(1302..1844)
                     /locus_tag="PK264_19655"
     CDS             complement(1302..1844)
                     /locus_tag="PK264_19655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HRD78124.1"
                     /translation="MARERTETDEERRSSLLPLRTGVGILLLDHRGRVFIGRRIPRWS
                     PEERYWHMPQGGVEAGENLKHAARRELAEETSIKDCEIVGKTDGWLRYDLPEELLGIA
                     LKGRYRGQRLRWYAMRFLGRDRDINLNPRGRPRAEFEDWRWEKPEAIAALTIPYKRRL
                     YEEVIEYFAAEIARHAPRKR"
     gene            complement(2011..2670)
                     /locus_tag="PK264_19660"
     CDS             complement(2011..2670)
                     /locus_tag="PK264_19660"
                     /inference="COORDINATES: protein motif:HMM:NF018960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRD78125.1"
                     /translation="MMRERTSRVLFAYWNEVRGERMAPRRFDIEPARISEVLSETFIL
                     EREEGTTRFRLAGTRIVEQLGHELRGIALEDLWADSDRRRLDTILASVVEQGGVGLVH
                     FTAASPPEDGIEPRSAAFEMLLLPLVHTEGTVTRILGAISNSAPPHWLGAVPLPDRTI
                     DSAMVIWPDGRPHSAIEAADRQAPFKPGYAAARIVRFDRRQFRVFDGGLDAGRSDRSE
                     K"
     gene            2798..3523
                     /locus_tag="PK264_19665"
     CDS             2798..3523
                     /locus_tag="PK264_19665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRD78126.1"
                     /translation="MDERQPALNVPWVIVALIGLLIAVHVWLWNLEAPATRWWLVALA
                     LIPIRFDGLAALLPGGTMASWTSLVTHMGVHADITHLVMNTTALLAFGGAVAKRVGTM
                     RFIAFTLVCGLAGAGLFLVLNLGGKTPMIGASGAVSGLVAGAFRFFLPHLETGGIESF
                     QRDPRATKLLSLAETLRNRRCQLAIGIWLIINFLMGLGGSVITGGAGIAWEAHLGGFL
                     AGLALMAPFDRPQVPEPVSFIAE"
     gene            3763..4190
                     /locus_tag="PK264_19670"
                     /pseudo
     CDS             3763..4190
                     /locus_tag="PK264_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            complement(4259..5371)
                     /gene="ald"
                     /locus_tag="PK264_19675"
     CDS             complement(4259..5371)
                     /gene="ald"
                     /locus_tag="PK264_19675"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HRD78127.1"
                     /translation="MRIGVPKEIKVQEHRVGMTPVSVREAVAHGHEVWVEANAAARQG
                     ISDEDFMKAGAKVIPTAAEIFAKCDLIVKVKEPQPAECRMLREGQTLFTYLHLAPDPQ
                     QAKELMTSGATAIAYETVTGPRGGLPLLAPMSEVAGRMAIQAGAHCLEMEQGGRGMLL
                     GGVAGVAPAKVVVLGGGVAGVNAVRMAVGLEAQVTVLDINLERLYALDQQFGAALTTI
                     FSTRAAVEDYVLDADLVIGAVLVPGASAPRLVSRKMVEAMKRGSVLVDISIDQGGCFE
                     TSRPTTHAKPTYIEHEVVHYCVANMPGAVARTSTFALNNATLPFILALADKGVGQALK
                     ADPHLMAGLNVHAGRITHANVASALGVEHWGAAFKT"
     gene            complement(5647..6396)
                     /locus_tag="PK264_19680"
     CDS             complement(5647..6396)
                     /locus_tag="PK264_19680"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRD78128.1"
                     /translation="MSSGPSEATVGHDRTRTLLKNGTFLGGLPDAALDLLARRVHVAK
                     FAKGATIYTRGDAGDSMMIIVTGRVKITNVTADAHEVVLNFLGKGDVLGEIALLDGRE
                     RTAGAVTLEATEVLVLQRRDALPVLTAHPDTLLEVIMVLCEKLRLASGIIESHTLDMA
                     GRTASGLLRLVRQHGRQSKGGALIDLKLSQRDLGNYLGLSRENVNRQLAALREKGVIS
                     VDGANITIVDEDALTAIAEHDGEDVGLKPRA"
     gene            6625..>6915
                     /locus_tag="PK264_19685"
     CDS             6625..>6915
                     /locus_tag="PK264_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78129.1"
                     /translation="MRSLHVALGIAPLLLFPTAVTACGPFCSDARTGADAYRSSVSGK
                     SLLLRVQAKGDEKACAADRESLAALIKAGDPEMVKMSMGFFTCEAVQDEAKKW"
BASE COUNT         1186 a         2276 c         2257 g         1180 t
ORIGIN      
        1 catcgccgcc gaggccgcgg cgttgcccgc gccgacgacg atgacgtcgg catcctgtcg
       61 atccttggcc atgagacgtg tcctgtggtg tccgctttat atcgtgcctg agacttacga
      121 tccgccgctc agccacgcaa cccggccgcg agcgcacccg cccccgatag aacgaggagc
      181 gtgaggaccg cggcccggaa ccggctctcg ccgagcgcgt tcgctgcccg ggccccgatc
      241 aacgttccaa gcgcggcagt cggcgcgagg aggagggtca ccaacgccgc ctcccggtcg
      301 aaagtgccgc ttgccgtgta gagcgccgtc acgaatgcat agcagagcgc gaagaacgtg
      361 agcgagagcg cgcgggtcac atcgcgcggc aggcggtcag cagcggcgag cagcatgagc
      421 ggcggccccg gcatgacgag aagggctgtc agaagtcccg aggcaattcc agtcgctccg
      481 gtcagcgcgg ggcgcggcga tgcctcggag cgcggcctgg caccgatcaa tccaatctcg
      541 cgggcaatga gcagcgcggc agcaagcagg atgacgatgc cgaccgcgat cttcaggacc
      601 gagaggtcga gccggagcag gatcgccaga ccgagcgggc agccgaccag cgcccccgcg
      661 gcgagcaggc cgagccgtct gcggtcgacg tgctgcagga ggcgcggcac catcatggcg
      721 ctctgcacaa ggtgcaggcc gaccagcacc gccatcgcgc gcgtcgagtt catcgcccac
      781 aggaacaccg gcgccgccag gatcgcatag ccgaacccgg ttgcggcctg caccagcgca
      841 ccgacgaagc tcgccgctcc gagcgccgcg acgagtggcc actcgaggct cacgcgcgcc
      901 ccgagaagac gaaggctgca ccgattgcga tgnnnnnnnn nnnnnnnnga tcgcctcctt
      961 gagccacacg accgagaaga cggcgaacac gaggagcgtg atcacctcct gcatggtctt
     1021 cagctcggcc gccgagtaga ggccgtggcc gaacctgttt gcgggcacgg cgaggcagta
     1081 ttcgatgaag gcgatgcccc acgagacaag gatcacgacg aaaagcggct gcgatttgta
     1141 ggagaggtgc ccataccagg cgagcgtcat gaagacattc gaggcgatca gcagcagcgc
     1201 cggtacaagg tagggccaga tccccgcaag tcctgtcatg tcgtactccc ccggcgcgct
     1261 cacggccgtc cgggagacta aagctcaatg ttggggccag ctcaacgctt gcgcggcgcg
     1321 tgacgagcga tctccgccgc gaaatactcg atcacctcct catagaggcg gcgcttgtag
     1381 ggaatcgtca gcgcggcaat cgcctcgggc ttctcccagc gccagtcctc gaactcggcc
     1441 cgcgggcggc cgcgcggatt gaggttgatg tctcggtccc ggccgaggaa gcgcatcgca
     1501 taccagcgga gccgctggcc gcggtagcgc cccttcagcg cgatgccaag aagctcctcg
     1561 gggaggtcgt agcgaagcca gccgtcggtc ttgccgacga tctcacagtc cttgatgctg
     1621 gtttcctcgg ctagctcgcg gcgcgcggcg tgtttcaagt tctcgccggc ctcgacgccg
     1681 ccctgcggca tgtgccagta gcgttcctcg gggctccagc gcgggatgcg tcggccgatg
     1741 aacacgcgcc cgcggtggtc cagaagcagg atgccgacgc ccgtccgcaa aggcagcagg
     1801 gagctccgcc gctcctcgtc tgtctccgtg cgttctcgtg ccattcagct gggtccaggg
     1861 ctccaacatc gcaattgaaa cgcatcgagc aggcattttg ttgctcgaca tcgaatccgc
     1921 atatcagccg gaaaggtcga cgatttcaag cagcaagacg agttatcggg atgaaaccgc
     1981 gatattgcga ggtacgaggc gaccgtagct ctatttctcg ctgcggtccg agcgccccgc
     2041 atcgaggccg ccgtcgaaca cacgaaactg tcgacggtcg aatcggacga tgcgcgccgc
     2101 ggcatagccg ggcttgaacg gcgcctgccg gtccgcagcc tcgatcgccg agtggggccg
     2161 cccgtccggc cagatcacca tcgcgctgtc gatggtgcga tccgggaggg ggacggcgcc
     2221 gagccagtgg ggtggcgccg agttcgagat cgcgccaagg atgcgtgtga ctgtgccttc
     2281 agtgtggacg aggggcagca gcagcatctc gaatgcggcc gagcgcggct ctatgccgtc
     2341 ctcgggcggg cttgcggcgg tgaagtggac gagaccgacg cctccctgct ccacgacgga
     2401 ggccagtatg gtgtcgagtc ggcggcggtc actgtccgcc cacaggtcct cgagcgcaat
     2461 gcctctgagc tcgtgaccga gttgctcgac gatcctcgtc ccggcgagac ggaaacgtgt
     2521 cgtcccctcc tcgcgctcca ggatgaacgt ctcggacaga acttccgaaa tgcgggccgg
     2581 ctcgatgtcg aaccgcctcg gagccatgcg ctcgccacgc acctcgttcc aataagcgaa
     2641 gagtactctg ctggtgcgct cccgcatcat gcggctcact gcctttcgtc gtgggtccgt
     2701 gtgagccaat tcggcacaat cggacgtaac atcgaaattc ttcgcttccg aagaggcagc
     2761 ttccatgcca agggcggacg tcacggagga gtgtggcatg gacgagcggc agcccgcatt
     2821 gaatgtgccc tgggtgatcg tggcgctgat cggcctcctg atcgcggtgc atgtctggct
     2881 gtggaacctt gaggccccgg cgacgcgctg gtggctcgtg gcgctcgcgc tgatcccgat
     2941 ccgcttcgat gggcttgcgg cgctcctgcc cggtggcacg atggcgagct ggacctcgct
     3001 cgtcacgcac atgggggtgc acgccgacat cacccacctc gtcatgaaca cgacggcgct
     3061 cctcgcgttc ggcggcgccg tcgccaagcg cgtcggcacg atgcgcttca ttgccttcac
     3121 gctcgtctgc ggacttgctg gtgccgggct tttcctcgtc ctcaatcttg gcggcaagac
     3181 accgatgatc ggcgcctcgg gagcggtttc agggctcgtc gccggcgcgt tccgcttctt
     3241 tctcccgcat ctcgagacag gcggcataga gagcttccag cgcgatccgc gcgcgacgaa
     3301 gctgctctcg ctcgccgaaa ccttacgcaa ccggcgctgc caactcgcca tcggcatctg
     3361 gctcatcatc aatttcctga tggggctcgg cggctcggtc atcacgggcg gcgccggcat
     3421 cgcctgggag gcccatctcg gaggcttctt ggcgggtctc gcgctgatgg ccccgttcga
     3481 caggccacag gtgccggagc cggtgtcgtt catcgcggag tgaccgatct cgcctgccgt
     3541 cgtcggggca gtcgatgatc atggtttgca cgggcgcaag aatcgccctt gcgctcgcaa
     3601 acaggtatgc cattctgtcg cagctgcagg cgccctgtat tgcgccgtgc catgcaccaa
     3661 agggagggac gaacccaccc ggagcgggca gttcgcgccc aaccgaaacc ggacttgctc
     3721 ggatttatcg aggggagtta acgcgtaaga ggagttcaca tcatggacgt tgcagcaatc
     3781 ctgaggcaga agggccgttc cgtcgagaca gcctcgccgg agacgacact cacggacatt
     3841 gccaagcgac gcagctcagg atcggcgcca tcgtcgtcgt cgacggtccc ggccgcgtca
     3901 tcggcatcat ctcggagcgg gacatcatcc gctgcatcgc gaccgagggg cctaatgcgc
     3961 tccggtcgcc cgtttcgagc gtgatgacga acaaggtgat cgtctgctcg ctcgaagatt
     4021 ccctggatga tgtgatggaa aggatgacgg cgcggcgctt ccgccacctg cccgtcgtca
     4081 aggacgatcg cctcgtcggc atcgtctcga tcggtgatgt cgtcaagcac cacatcgccg
     4141 aggtcgagca cgacgcgata gcgctccgcc agtacatcac gtcggtctga gcaacccgat
     4201 cggcgccaaa gcggccgaac tgaattcggt tcggccattc gtgacggctc gcgcgcgctc
     4261 acgtcttgaa ggccgctccc caatgctcga cgcccagcgc cgaagcgacg ttcgcatgcg
     4321 tgatacggcc ggcgtgaacg tttagcccgg ccatgagatg gggatcggct ttgagcgcct
     4381 gtcccacgcc cttgtcggcg agcgccagga tgaacggcag cgtcgcgttg ttgagggcaa
     4441 acgtcgaggt tcgcgccacc gcgcccggca tgttggcgac gcagtagtgc accacctcgt
     4501 gctcgatgta ggtcggcttc gcgtgggtgg tcggccgtga ggtctcgaag cagccgccct
     4561 ggtcgatcga gatgtcgacg agcacggagc cgcgcttcat ggcctcgacc atcttgcgcg
     4621 agacgagccg cggcgccgag gccccgggca cgagcaccgc gccgatgacg agatcggcgt
     4681 cgagcacgta gtcctcgaca gccgcgcgag tggagaagat cgtggtgagt gccgcgccga
     4741 actgctgatc gagcgcgtag agccgctcga gattgatatc gaggaccgtg acctgcgcct
     4801 cgagcccgac cgccatgcgg acagcattga cgcccgcaac gccgccaccc agcaccacta
     4861 cctttgccgg cgcgacgccg gcgacgccgc cgagcagcat cccgcgcccg ccctgctcca
     4921 tctcgagaca atgggcgccc gcctggatcg ccatgcgccc ggccacctcg ctcatgggcg
     4981 cgagcagcgg caacccaccg cgcgggccag tcaccgtctc gtaggcgatc gccgtggcgc
     5041 ctgacgtcat gagctccttg gcttgctgcg gatcgggcgc gaggtgcagg taggtgaaca
     5101 gcgtctgtcc ctcgcgcagc atccggcact cggccggctg cggctccttg accttcacga
     5161 tgagatcaca cttggcgaaa atctcggccg cggtcgggat gaccttggcg ccggccttca
     5221 tgaaatcttc gtcgctgatg ccctgccggg ccgcggcgtt ggcctcgacc cacacctcgt
     5281 gcccatgcgc cacggcttcg cgcacgctca caggcgtcat gccgacgcga tgctcctgga
     5341 ccttgatctc cttgggaacg ccgatccgca tcgctctctc cttcttgatc gtgctctgta
     5401 accggggtgg tgcgcagccg cccttcacgc tgacgctgcc tgccccgaac tcacgctccc
     5461 cgcgcggggc gggcaatgtc tggctagtgt cccgatcgcg aagttcgtta ccacgtgccg
     5521 agagctcgga cgaacttcgc gatcgataag gacactcgca atgtcatgag cttagtgtgg
     5581 cgtcatgttc ggaagttcgt atctcgagta tgccgcacag tgaattcgaa cttccgaacc
     5641 gtcacactag gctcgcggct tcaggccaac gtcctcacca tcatgctcgg cgatcgccgt
     5701 caacgcgtcc tcgtcgacga tcgtaatgtt cgcgccgtcg acgctgatga cacctttttc
     5761 gcggagcgct gcgagctgcc ggttcacgtt ctcgcggctg agacccagat agttgccgag
     5821 atcgcgctgc gacagcttga gatcgatcaa ggcgccgcct ttcgactggc ggccgtgctg
     5881 gcggacaagg cggaggaggc cgctcgccgt gcgcccggcc atgtcgaggg tgtgcgattc
     5941 aatgatgccc gaggcgagtc gcagcttctc gcagagcacc atgatgactt cgagcagcgt
     6001 gtccgggtgc gccgtaagga ccgggagcgc atcgcgccgc tggaggacga gcacctcggt
     6061 cgcctcgaga gtgactgcgc cagccgtccg ctcgcggccg tcgagcaggg caatctcgcc
     6121 gagcacatcg cccttgccaa ggaaattgag cacgacctcg tgcgcgtccg ccgtcacgtt
     6181 ggtgatcttc acacggcccg tgacgatgat catcatgctg tcgccggcat cgccgcgcgt
     6241 gtagatcgtc gcgcccttcg caaactttgc gacgtgaacg cgacgcgcca ggagatcgag
     6301 ggcggcatca ggcaatccgc cgaggaacgt gccgttcttc aggagggtgc gggtgcggtc
     6361 gtgaccgacc gtcgcttcgc ttggaccgct cgacatgggg gctcgctcgg ctggggctcg
     6421 ctcggctgaa ttcgctgccg gagcaggctg gagtaggagt ttcccattgt cgccggcccc
     6481 cgtccagcca aatgcgggcg acccgcggac gggacgatcg cgacgagcct gtgtttcaat
     6541 tccgtctcac gcttgcttgt agggggcaac ctgacgcaca ctgggggacc ggtgcagcag
     6601 attaaacatc gaggatcgag aacgatgcgc agcttgcacg tcgcgcttgg gattgctccc
     6661 ctcctattgt ttccaaccgc cgtcacagcg tgcggaccgt tttgcagcga cgcacgcacg
     6721 ggcgccgacg cgtaccgctc gagcgtctcc gggaaatccc tgctgctgcg cgtccaggcc
     6781 aagggcgacg agaaggcctg cgcggccgac cgggaatctt tggccgcgct catcaaggcc
     6841 ggtgatccgg aaatggtgaa gatgtcgatg ggattcttca cctgcgaggc tgtgcaggac
     6901 gaggccaaaa agtgg
//
LOCUS       DAORNH010000248         6913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8129_length_6913_cov_2.724847, whole genome shotgun sequence.
ACCESSION   DAORNH010000248 DAORNH010000000
VERSION     DAORNH010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8129_length_6913_cov_2.724847"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>446
                     /locus_tag="PK264_19690"
     CDS             <1..>446
                     /locus_tag="PK264_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78130.1"
                     /translation="TPMLSGDGSVRLASVIGRVRDAKAKAYRDAQCRIVPSSSDIAEI
                     RDFLAECGAGCPCAAGLTKRLAELEGQAAACEGEGLALERLAKRGAGALASLKALAAG
                     AACASVKAKAEALIASFDEDAKRAAVEAKRKADEAAEARRKANADA"
     assembly_gap    447..513
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <514..1472
                     /locus_tag="PK264_19695"
     CDS             <514..1472
                     /locus_tag="PK264_19695"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRD78131.1"
                     /translation="LVVPKSAAPGTLVRSWVAHTNSINSVAISPDGREIISASNDGTA
                     KRWDAATGRLLGTFGPIVGASEVVSFHPVSIARSGYTIATGHGDGVVRLWGLEKKNQL
                     LNTLEKHKGPVWDVALSPDGRKVVSVDHEGEVKIWDAVTGGLIRTLSPKVGWLETVDF
                     SPDGALIVVAGVGGRLLVYNVASGQQISSHKAHAETVSSAVFVRTGQFIVSGAYDKYV
                     VITDVLSGQVLKRLSGHTGYVRSVAVTSDARFIVSGSYDKTVKIWEYGSGQLIKSIPA
                     HDEIIESVALAPDGRWFVSAGCDGRAANKSCTRSDLRIWSMP"
     gene            1590..1778
                     /locus_tag="PK264_19700"
                     /pseudo
     CDS             1590..1778
                     /locus_tag="PK264_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2313..2531
                     /locus_tag="PK264_19705"
     CDS             2313..2531
                     /locus_tag="PK264_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78132.1"
                     /translation="MLKTMFASLVAAGALTSLVALGSAPSEGAGFSSRPPSAAKICKG
                     KGKDRQCLACVIADGIKDCFWYAAPSKG"
     gene            2604..3524
                     /locus_tag="PK264_19710"
     CDS             2604..3524
                     /locus_tag="PK264_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRD78133.1"
                     /translation="MSQFSTDDPVPGDRQSCEAIEQVIVPRARDLGGFEVRRALPAAT
                     RQMVGPFIFFDQMGPAEFLLTNGIDVRPHPHIGLATVTYLFEGEIFHRDSLGSALAIR
                     PGELNLMTAGRGIVHSERETSEARTKPRRLFGIQAWAALPKSHEERAPAFVHLGVGDL
                     PRIEGGGARVRLIMGSLYGETSQAEFPHPSFYAEAVLAPGAVLPLDPDYDERAIYIAS
                     GVIDIAGQSFEAGRLLTFRPGDRISVLATTNARLMLLGGEPMDGPRHIWWNFVSSSKD
                     RIAAAKADWSGRRFAIVPGDEVERIPLPGA"
     gene            complement(3529..4155)
                     /locus_tag="PK264_19715"
     CDS             complement(3529..4155)
                     /locus_tag="PK264_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HRD78134.1"
                     /translation="MLAISGCGQKPAFVSKPEPWRQDDEQACLSGGKVAETPFLVSRQ
                     SLGGPGSCGALRPYEMSAAAGGTVLMKPSAILRCPMIPVVERWVRTVLIPAARHHLER
                     DVVELKIGASYSCRPANHVFGARLSEHGHANAIDISRFLLSDGRWLTVKEGWHGSARE
                     RAFLRALHAGACREFSTVLGPDADVYHHDHFHFDLARHGRDGTIRICR"
     gene            complement(4770..5930)
                     /locus_tag="PK264_19720"
     CDS             complement(4770..5930)
                     /locus_tag="PK264_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HRD78135.1"
                     /translation="MRITAVRDIVAPIKSQIANAYIDFSAMTASVVAIEAELKGRRLV
                     GFGFNSNGRYAQPGLLRERFIPRLLAAKAEDLLDGNGLVDPIKSGAIMMRNEKPGGHG
                     ERSVAVGTLDMALWDLVAKAEGKPLWKVLADRYRNGKADANAWVYAAGGYYYPGKGLD
                     ALKDEMRSYLDRGYDTVKMKIGAVPLAEDMRRIEAVLAIVGKGERLAVDVNGRFDLET
                     GLAYGRALAPLGLRWYEEPFDPLDYRLHTVLADAYPGVLATGENLFSMQDARNLIRHA
                     GLRPDRDILQFDPALSYGLAEYLRTLAMLEAHGWSPRRCIPHGGHQFALNIAVGLGLG
                     GNESYPDVFAPFGGFADATPVEGGRIRMPDAPGIGFELKSNLWAVMKPLATA"
     gene            complement(6054..6130)
                     /locus_tag="PK264_19725"
     tRNA            complement(6054..6130)
                     /locus_tag="PK264_19725"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6094..6096),aa:Asp,seq:gtc)
     gene            6278..6913
                     /locus_tag="PK264_19730"
                     /pseudo
     CDS             6278..6913
                     /locus_tag="PK264_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951103.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT         1177 a         2207 c         2328 g         1134 t
ORIGIN      
        1 aaacgccgat gctctcgggc gacggcagcg tcaggctcgc gagcgtcatt ggacgcgtgc
       61 gcgacgccaa ggcgaaggcc tatcgcgatg cccagtgccg catcgttccc tcgtcgagcg
      121 acatcgccga gattcgcgat ttcctcgccg agtgcggcgc gggctgcccg tgtgcggcag
      181 gcctcacgaa gcgcctcgcc gagctcgagg gccaggccgc cgcctgcgag ggcgaagggc
      241 tcgcactcga gcggctcgca aagcgcggcg caggcgctct cgcctcgctg aaggcactgg
      301 cggcgggtgc cgcctgcgcg agcgtcaagg ccaaggccga ggcgctgatc gcgagctttg
      361 acgaggacgc gaagcgggcg gcggtggagg cgaagcggaa ggccgacgaa gccgccgagg
      421 cgaggcgcaa ggcgaacgcg gacgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctctcgt cgtgccaaaa agcgccgcgc
      541 ctgggacgct ggtgaggtcg tgggtggccc acacgaatag catcaacagc gttgccatct
      601 cgcctgacgg ccgagagatc atctccgcca gcaatgacgg caccgcaaag cgctgggacg
      661 cggcaaccgg tcggctactt gggacctttg gcccgattgt cggggcgtcc gaagtcgtct
      721 ctttccaccc agtttcgatc gcacgaagcg ggtatacgat cgcgacgggt catggtgacg
      781 gtgtcgtccg cctctggggc cttgaaaaaa agaaccaact cctgaatacg ctcgagaagc
      841 ataaggggcc cgtatgggac gttgccctgt cgcccgacgg tcgaaaggtg gtctcggtcg
      901 atcatgaagg cgaggtcaag atctgggacg cggtcaccgg agggttgatc cgtacactct
      961 ctccaaaggt cggctggctc gagacggtgg atttctcgcc tgacggtgct ttgatcgtcg
     1021 tcgcgggcgt cggtgggcgg ctccttgtct acaatgttgc ctcgggccag cagataagca
     1081 gccacaaggc gcacgcggag accgtatcgt cggccgtatt tgtccggacg ggtcagttca
     1141 tcgtttcggg ggcctacgac aaatacgttg tcatcacgga cgtgctctcc ggccaggttc
     1201 tcaaaaggct ctccgggcat acgggctatg tccgctccgt cgccgtcacg tccgatgcgc
     1261 gcttcatcgt ctccggcagc tacgacaaga ccgtcaagat ttgggagtac ggcagcggcc
     1321 agctgatcaa atcgatcccg gcgcacgacg aaataatcga atcggtcgcc cttgccccgg
     1381 acgggcgctg gttcgtcagt gccggctgtg atggaagagc ggctaacaag agttgcaccc
     1441 gcagcgactt aagaatttgg tcgatgccgt agaagtcaag actgaaggct gggttgagat
     1501 cttgcgaagc ccaaaacgca cgattgcctt ctgcgccgtt gggcagcgct gcgctcgaca
     1561 ccaacctgcc cacaccggtt ccccggcgag tgaaccgcga cagtggtacg atgcgaggac
     1621 agcgacatat cgcaggaggt cgccgacgtg tcaggcgtgc cctctccatg gcagcgctga
     1681 cagccatccg gctctgccca cgcttcaatc agttcgacca agcggtcaaa gccaggagcg
     1741 ttgccgccaa agtcgctgtc gttgccgtgg ctcgcaaacg ccccgtcacg ctcgacgcga
     1801 tgatccgaac cggccaaccc ttcaaggctt ggcaccattc tctagttcgg ccccggcgga
     1861 gccattctcc cgtcccggcc acacgccgat cgaccgccgt gcgcaggggc cgtcaaggac
     1921 gcgctcttcg cacgcccgtg aggtcttgtc ctttacggac ccgagctcgg cggtaaactg
     1981 atgcgacccg ggctaactga aatacagttg ccggacagtc cggtcacgct ggcgcgtgct
     2041 cggacttccg ggaaatcagt cgggtggtgg tgcggcggag ctcgctccgg ctacggctca
     2101 cttttgacgt ccacacgggt ttggggcgcc tctcgcgtag gcttggctac gccaactctc
     2161 ttgcggctga aaaaccactc actctcttga acctcgcgat ctgcgctgac gtctcattgc
     2221 tgagcagcac agagcacggt gctgccgacg actgcttcac cgccggtcga tatctctcgc
     2281 cggctcttgt ttagccatat tggagactga tcatgctcaa gaccatgttt gcttcgcttg
     2341 tcgccgcggg cgccctcacc tcgctcgtcg cgctcggctc cgccccctcc gaaggcgccg
     2401 ggttctcgtc gcggccgccg tcggcggcta aaatctgcaa gggcaaaggc aaggaccgcc
     2461 agtgcctcgc ctgcgtgatc gcggacggca tcaaggactg cttctggtac gccgccccct
     2521 caaaggggtg aggctctcca agggggcagg cattgactga gtagcgacgt cgtgggctca
     2581 tggcggacga caggaggtcc gccatgagcc agttctcgac cgacgacccc gtgcctggcg
     2641 atcgccagtc ctgtgaggcg atcgagcagg tgatcgtgcc gcgcgcccgc gatctcggcg
     2701 gcttcgaggt ccgccgcgca cttcccgccg cgacccgtca gatggtcggc cccttcatct
     2761 ttttcgacca gatgggcccg gccgagttcc tgctgacgaa cgggatcgat gtccgcccgc
     2821 atccccacat cggcctcgcg acggtcacgt atctgttcga gggcgagatc ttccatcgcg
     2881 attcgctcgg cagcgcactg gcgatccgtc cgggcgagct caatctgatg acggccgggc
     2941 gcgggatcgt ccattccgag cgggagacga gcgaagcacg gaccaagccg cggcgcctgt
     3001 tcggcatcca ggcctgggcg gcgctgccga aatcgcacga ggagagagcg cccgccttcg
     3061 tgcacttggg tgtgggcgat ctcccccgca tcgaaggcgg aggggcgcgc gtgcgcctca
     3121 tcatgggctc gctctacggc gagacgtcgc aggccgagtt cccgcaccct tcgttctatg
     3181 ctgaggcggt gctcgcgccc ggcgccgtcc tgccgctcga tcccgactac gacgagcgcg
     3241 ccatctacat cgcctcgggc gtgatcgaca tcgcggggca gagcttcgag gcggggcggc
     3301 tcctcacgtt ccgcccgggc gaccgcatct cggtgctggc gacgacgaac gcgcgcctca
     3361 tgctgctcgg cggcgagccg atggatgggc cgcggcacat ctggtggaac ttcgtgtcgt
     3421 cgtcgaagga ccggatcgcg gcggccaagg cagactggag cgggaggcgc ttcgcgatcg
     3481 tccccggcga cgaggtggag cgcattccgc tgccgggagc ctgagtgatc agcggcagat
     3541 gcggatcgtc ccgtcacggc cgtgccgggc aaggtcgaaa tggaaatgat cgtgatggta
     3601 gacgtcggca tcggggccga gcaccgtcga gaattcgcgg caggcgccgg cgtggagcgc
     3661 tcgaaggaag gcgcgctcgc gggctgagcc gtgccagccc tccttgaccg tcagccagcg
     3721 tccgtccgaa agcaggaatc gcgagatgtc gatggcgttg gcgtggccat gctccgagag
     3781 gcgggcaccg aaaacgtggt tcgccggacg gcaggaatag gaggcgccga ttttcagctc
     3841 gaccacatca cgctcgagat ggtggcgcgc cgccgggatc aggaccgtcc gcacccaccg
     3901 ctcgacgacc gggatcatag ggcaacgcag gatcgcgctc ggcttcatga ggacggtgcc
     3961 accggcggcc gcggacattt cgtagggccg gagcgcgccg caggagccgg gaccgccgag
     4021 cgactggcgg ctcacgagaa agggcgtctc tgcaaccttg ccgccggaga ggcaggcctg
     4081 ctcgtcgtcc tgccgccagg gctcgggctt ggagacgaac gctggcttct gcccgcaacc
     4141 gctgattgca agcatcgcca agacggtact cgatacgacg acgcgacgga ctgacatggg
     4201 gtgagtgtac ggaggaaggc tgaagggcgc gtaaaatccg attggattcg gcaatccctt
     4261 gcttaccttc cgcgcgcggc tagttttcgt ccggcctgcg gcggcagcat ggcggccggc
     4321 cgggcatcgg gcgcgagatc atgcgatcac ggatagaggc ccgcggggca caagcgaatc
     4381 gctcttcatc gcgtgccaat gacaaggtcg cggaacccgg catatgcttt ccacacgcga
     4441 gagctgtgat tcgagccggt cttaccgtcc ggctcggggt tctcgcgctg ttggtgcgtg
     4501 cccgcgagcg tattgcgcgg gctggtctcg tcgtcgcgtc ccgcagtcgg gttcggaggc
     4561 tctggcttcc caatcccggt ccctgcggaa ttccactgga ccgaggacca acccgagcca
     4621 tggtcggccg accataggct tcaaacctgg gtaccgacag atcccgcgag ccgcggattg
     4681 agccccgcag ctcagggacc gccaacaaac cgagcgagcc ggagcgccgc tgctccggct
     4741 cgctcattcg ttttggcgaa ggcctcgggc tatgccgtgg cgagcggctt catcacggcc
     4801 cagaggtttg acttgagctc gaagccgatg cccggggcat ccggcatgcg gatgcggcca
     4861 ccttcgaccg gtgtggcatc ggcaaaccca ccgaacgggg cgaacacgtc cggatagctc
     4921 tcgttgccgc cgaggccgag gccgacggcg atgttcaacg cgaactgatg cccgccgtgc
     4981 gggatgcagc gccgcggcga ccagccgtgg gcctcaagca tggcgagcgt gcgcagatac
     5041 tcggcaaggc cgtaggagag cgccgggtcg aactgcagga tgtcgcgatc gggcctgagg
     5101 ccggcatggc ggatgaggtt cctcgcgtcc tgcatggaaa agaggttctc gcccgtcgcc
     5161 agcacgcccg gataggcgtc ggcgagcacg gtgtgcaggc gatagtcgag cgggtcgaac
     5221 ggctcctcgt accagcgcag cccgagcggc gcgagcgcgc ggccgtaggc aaggccggtc
     5281 tcgaggtcga agcggccgtt cacatcgacc gcgagccgtt cgcccttgcc gacgatggcg
     5341 agcactgcct cgatgcggcg catgtcctcg gcgaggggca cggcgccgat cttcatcttc
     5401 acggtgtcgt agccgcggtc gagataggag cgcatctcgt ccttcagtgc atcgagcccc
     5461 ttgcccggat agtagtagcc gcccgcggcg tagacccagg cgttggcatc tgccttgccg
     5521 ttgcgatagc gatccgcgag caccttccag agcggcttgc cctcggcctt ggcgacgagg
     5581 tcccagagcg ccatgtcgag cgtgccgacc gcgaccgagc gctcgccgtg accgccgggc
     5641 ttctcgttgc gcatcatgat cgcgccggac ttgatcgggt cgacgaggcc gttgccgtcg
     5701 aggagatctt cagcctttgc ggcgaggagg cgcgggatga agcgctcgcg gagcagtccg
     5761 ggttgcgcat agcggccgtt cgagttgaag ccgaagccga cgaggcggcg gcccttaagc
     5821 tccgcctcga tcgcgacaac ggacgccgtc atcgccgaga aatcgatgta ggcgttggcg
     5881 atctgcgatt tgattggggc gacgatatcg cggacggcgg tgatgcgcat gagttggctc
     5941 caggatgggc agagccagtg atgcgctgga gccgaccggg tgtaaaggcg gccgcgagga
     6001 cggcgctggc gccgcggcgc atcgctgata tggatatttc ggttggttgg aaatggcgcg
     6061 agcgacggga cttgaacccg cggcctccgg cgtgacaggc cggcgctcta accaactgag
     6121 ctacgcccgc gcaactgcgc gcgttgccgc gcgccgaggg ttgatacggg agtggtcccc
     6181 tgaagtcaag gcagagatca cgggcggaac gcaggtctgg agcggcttcg tccgatgcgt
     6241 taagacgtcc ccaacaaaca cacccgagga gacacccatg ctgcagctga ccgaggccat
     6301 gagccggctc ggcaccgaga ccgcgttcga tgtgctggcg cgcgccgccg agctgcagcg
     6361 gcaggggaag gacatcatct cgctcggcat cgggcagccc gacttcaaga cgccggagca
     6421 catcgtcgag gccgctgtga aggcgctgcg cgatggccag cacggctata cgccggcgaa
     6481 cggcatcctg ccgctgcgcg aggcggtcgc cgcggacctc aagcgccgcc acgacgtgga
     6541 ggtctcgcca gaatcgatcg tcatcatgcc gggcggcaag ccgaccatgc atctcaccgg
     6601 tcaggcggcg gaacgactag cgccgtcctg atgttcggcg cgcccggccg cgagatcatg
     6661 tatccggacc cgggcttccc gatctatcgc tcgatgatcg agtacacggg ggcgaagccg
     6721 gtgccgatcg cgctccgcga gacgaacgag ttcgccttct cggcggatga ggtactggcc
     6781 ggcatcacat cgaagacgag cctcatcatc ctgaacagcc ccggcaaccc gaccggtggc
     6841 gccgtcccgg aggccgagat caggaagctc atcgccggcc tcgcgaagca cccgaacgtt
     6901 gccgtcatgt cgg
//
LOCUS       DAORNH010000249         6875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8178_length_6875_cov_2.633578, whole genome shotgun sequence.
ACCESSION   DAORNH010000249 DAORNH010000000
VERSION     DAORNH010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8178_length_6875_cov_2.633578"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PK264_19735"
     CDS             <1..319
                     /locus_tag="PK264_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78136.1"
                     /translation="LGLSAVLATAALAQSTPTAPPPTAAKPEPPATPGAAPAPQTVAN
                     TDECLKAAANLVQIAESKKLGDDKLDKLEELLTKMEGQCDASQFTEAMATAGDIKVMI
                     DAN"
     gene            498..2822
                     /locus_tag="PK264_19740"
     CDS             498..2822
                     /locus_tag="PK264_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRD78137.1"
                     /translation="MDGPRRTFAFTFGLERLGLLALRAPLLSVLLILVLSALAVLGVM
                     RLKVDDSLSELFRTRTPEFARYEEIDKRFPSSEFDVLVVVEGKGLLERKGLEAFARMT
                     TELTIADGANGVVSMLSARAKPDEKGFSAQIITDPLPEGAAFDEAVKSLRSNDIVAGK
                     FLSADGQLALAVISLEREEIEKRGAREIIGNLDRLVAKELAGSGLTAKLTGAPVMQLE
                     IRNAVERDRLVYNGLGFLFGALLAFLYFRRVSLTLIAVLGPSIAILWTLGVIGGLDYR
                     LNLFINVLTPLILVSGFSDSMHLVFAIRRDIAVGIDRLAAARNAILDVAPACLLTAMN
                     AALALVSFSWADSALIRTFGFAALLAVAISYIAVAVVVPTLAALLIRPEPAIATAVDA
                     EQGTDNGVLARLTRGLAAMVLRAPMVFAFIGIAAVIATGLAYVRLEPHYRLADQVPDK
                     EQALAATGKLDQKLTGANPVHIMIQWKGGQGLYDPATLKTIGEAHNVLEKAAGLGNVW
                     SLESLRRWLREAGDDRIETVKSYVKILPSHLQRRFIAKEENAVLVTGRLPDIDASKIL
                     PIIDAIDAALTPVRKANPGYDISVTGLPAIAAKSSAKLIGELNWGLVGDMFVIFIFLG
                     IALRSALSGVASILPSLFPIFATGAILYATGEGLQFASIIAITVAFALAIDSTIHFLN
                     RFQLEEMREGADQGYHAALMRTVTHIGPAVVLTTLVLALGLGVTMLSHLPSLRLFGRL
                     AAVCLLASLIGQLIILPATIAVLRRWWPAKAAAR"
     gene            2952..3830
                     /locus_tag="PK264_19745"
     CDS             2952..3830
                     /locus_tag="PK264_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78138.1"
                     /translation="MIWWARDMGLASGLIESSTSAGGAENGELRDVVLTARALRAEIY
                     HLARLVTVVTSLLNALASGRRFGSGIDPMHYLPMRPLVLPAVLGRLTELQPAPACLAT
                     LASLELGLDRAISSLTARAKQPIGTSLGSCTALWRALAAELCSAIGEIDQLEELDGLS
                     ADDMSSELLLRYLSMVRDGQAPILDANGRVLLPVWKERRVDPRYVRPQPAWILRGEAR
                     QRIGLLDISRSGAGFAGVMHVTIGDRLAIELRGGRRIDGIVIWSSGDRCGTRHMQSLS
                     PTDPLLVSGCYRETGT"
     assembly_gap    3843..3945
                     /estimated_length=103
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4465..5796
                     /locus_tag="PK264_19750"
     CDS             4465..5796
                     /locus_tag="PK264_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRD78139.1"
                     /translation="MSPCSGGLEHIAHHDMLTGLFNRHGLAERLDRALAQCDAGDSFA
                     FHVIDLDRFKDVNDMYGHMVGDGMLKEVGARLRHATRGEDVVARIGGDEFAVLQSGAA
                     TSAEASAMATRIVDIIRQPFETEGKVIEVGGRVGTALAPRHGTTREALLRAADLALWS
                     AKSGGGRSTREYAPEMSEAVQHRRALEADLAKALAGGEFEVHYQPIYSLASRQVTGSE
                     ALIRWRHPLKGLISPAEFIPLAEETGLIAAIGRHVVRVACAEAATWPDQMKIAVNLSP
                     VQIRADSVVEDICEALEAAALAPSRLELEITETALLRSDDRTRARLDALEEMGIGMSM
                     DDFGTGYSSLGYLRRFAFDKIKIDRSFVAEIGRSADCRAIIQAITSLAASLGMTTTAE
                     GIETAEQLAELTALGCTEGQGYLLARPMPASQLHAFLAEAARRPEAAASAA"
     gene            complement(5976..6311)
                     /locus_tag="PK264_19755"
     CDS             complement(5976..6311)
                     /locus_tag="PK264_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78140.1"
                     /translation="MISSIEPTGESHGLSGPGRPRALSEADAVDIWIARWLRVRRKEL
                     LHRYGCDPRRLYDIWEEARFRGSRAKAEALFRERYPTLQDRIDWGPHRRIPRRPPPEL
                     QTSLLDLLK"
     gene            6431..>6875
                     /locus_tag="PK264_19760"
     CDS             6431..>6875
                     /locus_tag="PK264_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRD78141.1"
                     /translation="MILYPAIDLKDGACVRLERGAMEKATVFNGDPGAQARVFEAQGF
                     EWLHIVDLNGAFAGKPVNAGAVEAILKTVSLPCQLGGGIRDLATIDAWLSRGIARAII
                     GTAAVRDPALVKAACKAFPGRIAVGIDARGGKVAVAGWAETSELGA"
BASE COUNT         1088 a         2354 c         2159 g         1171 t
ORIGIN      
        1 gctcggcctc agcgccgtcc tggcgacagc ggccctcgca cagtcgacgc cgacggcgcc
       61 gccgccgact gcggccaagc ccgagccgcc ggcgacccct ggcgccgccc ccgctcccca
      121 gaccgtcgcc aataccgacg agtgcctgaa agccgcggca aacctggttc agattgccga
      181 atccaagaaa ctcggcgacg acaaactcga caagctcgag gagctgctga ccaagatgga
      241 aggccagtgc gacgccagcc agttcaccga ggcgatggcg acggccggcg acatcaaggt
      301 gatgatcgac gcgaactgat cacggcaatc gcaccctgat cggctcgatc gggaaagggc
      361 ggccccgagg gctgcccttt tttgcgcgcg gcgggccgcc caggactgcg atgcccgcgc
      421 caacttgccc gttgtgtttg ccgcccgtgg atgcgagccc tagcggaaat cgtagaatcg
      481 ggggaggcag gctcgacatg gacggtccgc gccggacatt tgccttcaca ttcggcctcg
      541 aaaggctcgg cctcctggcg ctgcgggcgc cattgctgtc cgtcctgctg atcctcgtcc
      601 tgtccgccct ggccgtgctc ggcgtcatgc ggctcaaggt cgacgacagc ctttccgagc
      661 ttttccgtac gcgcacgccg gagttcgccc gctacgagga gatcgacaag cgatttccct
      721 cgagcgagtt cgacgttctg gtggtcgtcg aggggaaggg cctccttgag cgcaaggggc
      781 tcgaggcctt cgcccgcatg acgaccgaac tcacgatcgc cgacggcgcc aatggcgtcg
      841 tgtcgatgct ctctgcccgc gccaagcccg acgagaaggg cttctccgcc cagatcatca
      901 ccgatcccct gcccgagggt gccgcattcg acgaggcggt gaagtcgctg cgcagcaacg
      961 acatcgtcgc cggcaagttc ctctccgccg acgggcagct cgcactcgcg gtcatttcgc
     1021 tggagcgcga ggagatcgag aagcgcggcg cacgcgagat catcggcaat ctcgaccggc
     1081 tcgttgcgaa ggagctcgct ggctccgggc tcacggcgaa gctcaccggc gcgcccgtga
     1141 tgcagctcga gatccgcaac gccgtggagc gcgatcgcct cgtctacaat ggcttgggct
     1201 tcctgttcgg cgcgctgctc gccttcctct acttccgacg cgtctcgctg acgctgatcg
     1261 cggtcctcgg cccctcgatc gccatcctgt ggacgctcgg cgtgattggc gggctcgact
     1321 atcgcctgaa cctattcatc aacgtgctga cgccgctgat cctcgtctcg ggcttctcgg
     1381 acagcatgca tctcgttttc gcgatccgcc gcgacattgc cgtcgggatc gaccgcctcg
     1441 ccgccgcgcg caacgcgatc ctcgacgtgg cgcccgcctg cctgctgacc gcgatgaacg
     1501 cagccctggc actcgtgtcg ttctcgtggg cggattcagc gcttattcgt acgttcgggt
     1561 tcgcggcgct actcgcggtg gcgatctcct acatcgccgt cgccgtggtc gtgccgaccc
     1621 tcgcagcact gctcatccgc cccgagcccg ccatcgcaac agcagtcgat gccgagcaag
     1681 gcaccgacaa cggcgtgctc gcccgcctga cacgggggct cgccgccatg gtgctgcgcg
     1741 ctccgatggt gttcgccttc atcggcatcg ccgctgtgat cgccaccggc ctcgcctacg
     1801 tccgcctcga gccgcactac cgcctcgccg accaggtgcc cgacaaggag caggcgctgg
     1861 cggccaccgg caagctcgac cagaaactca ccggggccaa ccccgtccac atcatgatcc
     1921 agtggaaagg tggccagggc ctctacgatc cggcgacgct caagacgatc ggggaagcgc
     1981 acaacgtgct ggagaaggcc gccggcctcg gcaacgtctg gtcgctcgag agccttcggc
     2041 gttggctgcg tgaggcgggg gacgaccgca tcgagacggt caagtcctac gtgaagatcc
     2101 tgccgtcgca cttgcaacgc cgcttcatcg ccaaggagga gaacgccgtc cttgtgacgg
     2161 gccgtctacc cgatatcgac gcgagcaaga tcctgccgat catcgacgcg atcgatgcgg
     2221 cccttacccc cgtgcgcaaa gccaatcccg gctacgatat ctcggtcacg ggactgccgg
     2281 cgatcgccgc gaagagctcg gccaagctca tcggcgagct caactggggt ctcgtcggcg
     2341 acatgttcgt gatcttcatc ttcctcggca tcgcgctccg ctcggcgctg tcgggtgtcg
     2401 cgagcatcct gccgtcgctc ttccccatct ttgcgaccgg cgctatcctc tatgcgaccg
     2461 gggaaggact gcaattcgcc tccatcatcg ccatcaccgt cgcctttgcg ctggcgatcg
     2521 attcgaccat tcatttcctc aaccgtttcc agctcgagga gatgcgcgag ggtgccgacc
     2581 aaggctacca tgccgctctc atgcggaccg tgacgcacat aggcccggcg gtggtcctga
     2641 cgaccctcgt ccttgccctt ggactcggcg tgacgatgct ctcccacctt ccctcgctgc
     2701 gcctattcgg gcgtctcgcc gcagtctgcc tactcgcctc gttgatcggc cagctgatca
     2761 tcctgccggc gaccatcgcc gtcctgcgcc gctggtggcc cgccaaggcg gcggcccggt
     2821 gatgccgcgg agcttggcct tgccggcgct acctcacacc gccgatcgcg cttgagacac
     2881 aattttaacg aatgaaatct gcgaaataag cactgtttca atgattttca gcgatcgtcc
     2941 atcgagtaca gatgatttgg tgggctcgcg atatgggtct tgcttccggg ctgatcgaaa
     3001 gtagtacatc tgcgggcggc gccgaaaacg gcgagctgcg cgatgttgtt ctgaccgcgc
     3061 gcgcactccg tgccgagatc tatcaccttg cgcgcctggt gacggtcgtc accagcctgc
     3121 tcaacgcgct cgcgtcgggg cgccggtttg gatcgggaat cgatccgatg cactacctgc
     3181 cgatgcgccc gctggtcctc ccggctgttc tcggccggct caccgagctc cagcccgccc
     3241 cggcctgcct ggcgacactc gcgagcctcg aactcggcct cgatcgggca atttcgtctc
     3301 tcaccgcgcg ggcgaagcaa cctatcggca catctctcgg ttcatgcacg gcgctctggc
     3361 gtgcgctggc ggccgagctc tgctccgcga ttggcgagat cgaccagctc gaagagctcg
     3421 acggcctgag cgccgacgac atgtcatcgg agctgctgct cagatacctt tcgatggtcc
     3481 gtgacggcca ggcgccgatc ctcgatgcca acggccgcgt gctgctgcct gtctggaagg
     3541 agcgccgggt cgacccgcgc tacgtccggc cgcagccagc ctggatcctg cgcggcgagg
     3601 cgcgccagcg catcggcctc ctcgacatct cgcgctcggg cgccggcttc gcaggcgtca
     3661 tgcatgtcac aatcggcgat cggctggcga tcgagctgcg cggcgggcgc cgcatcgacg
     3721 gtatcgtcat ctggtcgtcc ggcgatcgct gcggcacgcg ccacatgcag agcctgtcgc
     3781 cgacggatcc gctgctcgtt tcgggctgct accgggaaac aggaacctga ccatgtcgac
     3841 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctcga cgtttccccg
     3961 gcctcgagca ccacgcgctg tcgccgcatt ggcttgtcgt gctcgtcctc atcacggtcc
     4021 tgatcggtat cggcacgctt tcgcttgttc gccgcaacag ccgcaatgtc gtcacgcgcg
     4081 aggccatgcg ccggatcggc cgccgcgcgc gcgacaagtc gcgcgagctc gaggcgacgc
     4141 tagagaacat gggcgagggc atcatgatgg tcgatggcgg tggccagatc cgcgtcatga
     4201 accggcgcct caccgacatc ctcgacctca gcgccgagtt cacgtcgcgt cgtcgcacct
     4261 acccggagct gatcgcctgg cagcgcgacc aaggcgagtt cgcgagcctc gtcggcaagg
     4321 acgcagacca catcctgaag cacgtcggcg atccgatcgg ccagccgctc aggaccaagt
     4381 atctccgcca gcggcccgac ggcaccattc tcgaggtgcg cacccgcatg ctcgccgacg
     4441 gcggcttcgt gcgcacattc acagatgtca ccatgcagcg gcgggctcga gcatatcgcg
     4501 catcacgaca tgctgacggg gctcttcaac cgccatggcc tcgccgagcg gctcgaccgg
     4561 gcgctcgccc agtgcgatgc cggcgacagc ttcgccttcc acgtgatcga tctcgaccgc
     4621 ttcaaggacg tgaacgacat gtatggccac atggtcggcg atggcatgct gaaggaggtc
     4681 ggtgcgcgac ttcgccatgc gacccgcggc gaagacgtcg tcgctcgcat cggcggcgac
     4741 gagtttgccg tgctccaatc aggcgccgcg acgagcgccg aggcgtctgc catggcaacg
     4801 cgcatcgtcg acatcatccg ccagcccttc gagacggaag gcaaggtgat cgaggtcggc
     4861 gggcgggtcg gcacggctct cgcgccgcgc cacggaacaa cgcgcgaggc actgttgcgc
     4921 gcggccgacc tggccctctg gtcggccaag tcgggcggcg gtcggtcgac cagagagtac
     4981 gcgcccgaga tgagcgaggc ggtgcagcat cgccgtgcgc tcgaggccga tctcgccaag
     5041 gcgctcgcgg gtggcgagtt cgaggtccac tatcagccga tctacagcct cgcgagccgg
     5101 caggtgacag gcagcgaagc cctgatccgc tggcgccatc ctctgaaggg tcttatttcg
     5161 ccggcagagt tcatcccgct cgccgaggag accgggctca tcgccgcgat cggacgccat
     5221 gtcgtgcgcg ttgcctgcgc tgaggccgcg acctggcctg accagatgaa gatcgccgtg
     5281 aacctgtcgc ccgtccagat ccgcgccgac agcgtcgtcg aggacatctg cgaagcactc
     5341 gaggcggcgg cgctggcgcc gtcgcggctc gagctcgaga tcaccgagac ggcactcctg
     5401 cgcagtgacg accgcacgcg tgcgcgcctc gatgcgctgg aagagatggg gatcggcatg
     5461 tcgatggacg acttcggcac gggctattcc tcgctcgggt atctgcgccg cttcgccttc
     5521 gacaagatca agatcgatcg ctcgttcgtc gccgagatcg gccgctcggc cgactgccgg
     5581 gcgatcatcc aggcgatcac cagtctcgcg gcgagcctcg gcatgacgac cacggctgaa
     5641 ggcatcgaga cggccgagca actcgccgag ctcacagcgc ttgggtgcac ggaggggcag
     5701 ggctacctgc tcgcccggcc gatgccggcg agccagctgc atgctttcct cgcggaggcg
     5761 gcgaggcggc cggaggcagc ggcgagcgct gcctgatccc ggctagtgtg gcggttcgga
     5821 agttcgtatt caccccgcgg gacgctcagg aaacgaactt ccgaacacat cgccacacta
     5881 agctcatgtc attgctagtg tccttgtcga tcacgaagtt cgtccgaact ctcggcaagt
     5941 ggtaacgaac ttcgcgatcg ggatactagg cgcgactact tcagcaggtc gagaaggctc
     6001 gtctgcaact cgggcggggg gcggcgcgga atgcgacggt gcgggcccca gtcgatgcgg
     6061 tcctgcagtg tcggatagcg ctcacgaaac agtgcttcgg ccttggcccg gctgccgcgg
     6121 aaacgtgcct cctcccagat gtcgtagagg cgccgcgggt cgcagccgta gcggtggagc
     6181 agctccttgc gccttaccct cagccatcgc gcgatccaga tgtcgacggc atcggcctcg
     6241 ctcagcgcgc gaggacgccc cgggccgctc agcccgtgac tctctccggt tggctcgata
     6301 ctcgagatca tcactgtcct gcacaaaatt gtgccgggcc aactgccggc ttgttcccgg
     6361 attgatctca tggcatcgca gcaaacttgt ggcaatcata cccgcgtgct gccgaccgag
     6421 gacgagcccc gtgatcctgt accctgccat cgacctgaaa gacggcgcct gcgtccgcct
     6481 cgagaggggc gccatggaga aggcgacggt cttcaatggc gatccgggcg cacaggcccg
     6541 cgtgttcgag gcgcagggct tcgaatggtt gcatatcgtc gatctcaacg gcgcctttgc
     6601 ggggaagccc gtcaacgcgg gcgctgtcga ggcgatcctg aagacggtca gcctgccctg
     6661 ccagctcggc ggcggcattc gcgacctcgc gaccatcgac gcttggctca gccgcggcat
     6721 cgcccgcgcg atcatcggca cggcggcggt gcgcgatccc gccctcgtca aggccgcctg
     6781 caaggcgttt ccgggccgga tcgccgtcgg catcgatgcg cgcggcggca aggtcgccgt
     6841 cgccggatgg gccgagacct cggagctcgg cgcgg
//
LOCUS       DAORNH010000250         6835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8249_length_6835_cov_2.673156, whole genome shotgun sequence.
ACCESSION   DAORNH010000250 DAORNH010000000
VERSION     DAORNH010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8249_length_6835_cov_2.673156"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1295
                     /locus_tag="PK264_19765"
     CDS             111..1295
                     /locus_tag="PK264_19765"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD78142.1"
                     /translation="MQFYFENLYVSSFYASWSETIETLWRGQLIEAASHANGPKFAVP
                     ETCKPAVKPPHIILIHQESVVQPSLFPTLRYDKAVDEFFKSDDGRLHALRVETYGGAS
                     WLTEFSILAGLSTHSFGGMRQFVQAVMQGRIRDTLPEALRRCGYRNVVFYPMLRNFVS
                     NDKFYKSVSLDEIYDLKDQGAKRANERDRFYFGNALTFMEKHMKERAGAPLFTFIQTM
                     SAHWPYDVTYEPDEKVAGGGPGTDAEMHEYLRRLSIAQKDFDWLLAELKARFPGERFL
                     VLHYGDHHPMSTRMLLGFREDLEAEDVTLRPDSPGFITYYAARGLNYTVPALPKLEAL
                     DVPYLGTILLQAAGLPLSDAHRERLRLLAACDGRYYTCRSRGEILAFHRRLIDSRIMD
                     AR"
     gene            1325..2389
                     /locus_tag="PK264_19770"
     CDS             1325..2389
                     /locus_tag="PK264_19770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRD78143.1"
                     /translation="MPLDDLRAGSDPLPPYGRGGRSIALTVVVPVLNEERNLAALVQR
                     LVPVLEGLTERFEIIFVDDGSSDRTLAVIAEANLREPRLKAISLSRRFGKEIALSAGL
                     RRAVGDAVIMIDADLQHPPEVIRDMVARWREGYDVVYGQRRDRESDSRLRRAFAHGFY
                     GLFRSISGTSMPEGAGDFRLLDRRAVNAFNQLGERARFNKGLYAWIGFKSVGVPFEVA
                     ERHAGASRFRFASLTRFALDGLTSFSTVPLRISTYLGLAVSSAAFLYALIFLAKTLFK
                     GIDQPGFPTTIIAIMLLGGIQLISLGVIGEYLGRVYEEVKARPLYIEAETVGALGVIS
                     PVADGTGRPHTSSGSGAGQR"
     gene            2389..2796
                     /locus_tag="PK264_19775"
     CDS             2389..2796
                     /locus_tag="PK264_19775"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRD78144.1"
                     /translation="MTRGPGRHPTAHIAGFLASGGLAFTVDAGVLLLATQIGGLDPFI
                     ARALGISAAMIAGWLAHRSWTFAVAARPTFKEFLHYAALQAGSIASNYAIYAAILLLR
                     PATEPLVALFVSSAVAAVIAYVGMRLGVFRRAR"
     gene            complement(2831..3475)
                     /locus_tag="PK264_19780"
     CDS             complement(2831..3475)
                     /locus_tag="PK264_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD78145.1"
                     /translation="MPQRLTIWTFDWVPKGPRGHVRDLRLRWALEEAGILYDVATVPF
                     QDRGPEHLARQPFGQVPFLEDGDIRIFESGAGLLHIAEKSDALMPRDPKGRADTLQWL
                     VAGLNSIEMVTVPWWFINLGKPPSNPLAGWMKQRFERLETVIAGREWLAASRFTVADI
                     MMADALRVPRNLGELGSHPALMAYVARALDRPAFKKALVDQLAHFEAADAKRPN"
     gene            complement(3525..4145)
                     /locus_tag="PK264_19785"
     CDS             complement(3525..4145)
                     /locus_tag="PK264_19785"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HRD78146.1"
                     /translation="MAHLDLAKLKAATLNTEPYHYTIVPGFLAPDMVRRINATYPPIA
                     AGGSYPIESLSESMAIKEVIDELDGPAFEAAIAEKFAVDLSGRPKMYSLRGYTRAKDG
                     QIHTDSRDKIITVLLYLNDTWQQPGGRLRILKNGHNVDDFVAEVPPDNGTLLVFKRSD
                     TSWHGHHPFEGQRRSLQMNWMTSEGSKGWHKVRHSVSAAIKKVMAK"
     gene            complement(4206..5231)
                     /locus_tag="PK264_19790"
     CDS             complement(4206..5231)
                     /locus_tag="PK264_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HRD78147.1"
                     /translation="MQRPDTASSALRGRRGWLITDDKIGMIVQARGVADALGLDYEWK
                     SVSPQGVWRLMAPWGPVAPAEKFGAPGTPFAPPFPDVALATGRLAIPYLRALRKASGV
                     GTYCVVLQDPRTSASIADLICVPEHDRRRGPNVLTTLVPAHSFTEARLAKLRASTPAD
                     IAALPSPRVAVILGGKSDAFKYTEADDDTFAASLATFARLGASFMITPSRRTHKRLLD
                     CVGKATEGRPRILWRGEGENPYPQFLAHADQLIVTGDSANMTGEACATGRPVYVFVPP
                     TVSPKFARFHDALRRYGATRPLSPAMTSFETWSYAPLDAAKEIAAEIERRWLRRQAMI
                     PGLLQRA"
     gene            5323..5796
                     /locus_tag="PK264_19795"
     CDS             5323..5796
                     /locus_tag="PK264_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRD78148.1"
                     /translation="MRHDLDATDWRILRELMADARISNVTLSARVGLSPAPCLRRVRA
                     LEEAGVIRGYHAHINGPRAGFDLVAYAMVRLHAQAEADLRAFENLVLTWPLVREALML
                     SGDVDYMLKCIAPDLPTFQDFVLRKLTAAPNVASVKTALAIRVAKDEPGVPLMFS"
     gene            complement(5974..6471)
                     /locus_tag="PK264_19800"
     CDS             complement(5974..6471)
                     /locus_tag="PK264_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRD78149.1"
                     /translation="MFFDIHNPEFWVAVAFCGFLALLAWFKVPAALGRALDQRAVAIR
                     KELDEARKLREQAQELLDEYTRKQRLADDEAQAIVANAEREAAAMKVAAEKSLKESLE
                     RRARLAEEKIGRAEQQAVAEVRAAAIDAAVVAAERILKAKVGTTAAGAPLVDQAIRDL
                     KGRLN"
     gene            complement(6477..>6835)
                     /locus_tag="PK264_19805"
     CDS             complement(6477..>6835)
                     /locus_tag="PK264_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HRD78150.1"
                     /translation="LSDTGRQARLHQESGYLPITKAAYEKSIADARGRAQNHAKEARD
                     RVAQASDKQRAELEASLAAKMAETEKRIAAMKSAATASIGAIAADTAGPLVRKLIGVD
                     PTADEVRSALQPAAGE"
BASE COUNT         1059 a         2301 c         2263 g         1212 t
ORIGIN      
        1 gtcaagccgt agagcagcgc cagcgagaag cagacggcga gcatggtctc catgccgctc
       61 atggcgtgcc aggcgaaggc accgctcagg ccaaaggcga gcgccgccac atgcagttct
      121 acttcgagaa cctctacgtc tcctcgttct acgcctcctg gagtgagacc atcgagacgc
      181 tctggcgcgg ccagctgatc gaggccgcaa gccatgccaa cggtccgaag tttgctgtcc
      241 cggagacctg caaaccggca gtcaagccgc cgcacatcat cctcatccac caggaatcgg
      301 tcgtccagcc gagcctcttt ccgacgcttc gctacgacaa ggcggtcgac gagttcttca
      361 agtcggacga cggccgcctg catgcgctgc gcgtcgagac ctacggcggc gcctcgtggc
      421 tgaccgagtt ctcgatcctc gccggtctct cgacgcactc cttcggcggc atgcgccagt
      481 tcgtgcaggc cgtgatgcag ggccgcatcc gcgacacgct gcccgaagcc ttgaggcgct
      541 gcggctaccg caatgtcgtc ttctatccga tgctgaggaa tttcgtttcc aacgacaagt
      601 tctacaagtc ggtgtcgctc gacgagatct atgatctgaa ggaccagggg gcaaaacgcg
      661 ccaacgagcg cgatcgcttc tacttcggca atgcgctcac cttcatggaa aagcacatga
      721 aggagcgggc cggtgcgccg ctcttcacgt tcatccagac catgtcggcg cactggccgt
      781 atgatgtcac ctacgagccc gacgagaagg tcgcgggcgg cggacccggc acggacgctg
      841 agatgcacga gtatctccgc cgcctgtcga tcgctcagaa ggacttcgac tggctcctcg
      901 ccgaactcaa ggcgcgtttc ccgggcgagc gcttcctcgt cttgcactac ggcgaccacc
      961 acccgatgtc gacgcgaatg ctgctcggct ttcgcgagga cctcgaggcc gaggatgtga
     1021 cgctccggcc cgattccccg gggttcatca catactacgc ggcgcgcggg ctcaactaca
     1081 ccgtgccggc gctgccgaag ctcgaggcgc tggacgtccc ctacctcgga accattcttc
     1141 tgcaggcagc gggcctgccg ctttcggatg cccaccgcga gcggctgcgg ctgcttgccg
     1201 cctgcgatgg ccgctactac acctgcagga gccgcggcga aatcctggcc ttccaccgcc
     1261 ggctcatcga cagccgcatc atggatgcgc gctgagacat gcctgcaaat gacggacaac
     1321 cgagatgccg ctcgacgatc tgagggccgg gtccgatccg ctgccgccct acggccgcgg
     1381 cggacggtcg attgcgctca cagtcgtcgt gcccgtgctc aacgaggagc gtaatcttgc
     1441 tgcactcgtg cagcgcctgg tacccgtgct cgaggggctc acggagcgct tcgagatcat
     1501 cttcgtcgac gatggctcga gtgatcgcac tctcgccgtg atcgccgaag cgaaccttcg
     1561 cgagccgcgc ctcaaggcga tttcgttgag ccgccgcttc ggcaaggaga tcgcgctctc
     1621 tgcggggttg cgccgtgcgg ttggcgatgc cgtgatcatg atcgatgccg atctccagca
     1681 tccccccgag gtgatccggg acatggtggc gcgctggcgc gagggctacg atgtcgtcta
     1741 cggccagcgc cgcgaccgcg agagcgacag ccgcctccgc cgcgccttcg cgcacggctt
     1801 ctacggtctc ttccgttcga tcagcgggac cagcatgccg gagggtgccg gcgatttccg
     1861 cctgctcgat cgccgcgcgg tcaacgcctt caatcagctc ggcgagcgcg cgcgcttcaa
     1921 caaggggctc tatgcctgga tcggcttcaa gtcggtcggc gtgccattcg aggtcgccga
     1981 acgccatgcc ggtgcttcgc gcttccgctt tgcctcactc acgcgcttcg cactcgacgg
     2041 cctcacctcg ttctcgaccg tgcccttgcg catatcgacc tatctcgggc tcgcggtctc
     2101 ctcggcggcc ttcctgtatg ccctgatctt cctcgccaag acgctcttca agggcatcga
     2161 ccagccgggc ttcccgacga cgatcatcgc catcatgctg ctcggcggca tccagctcat
     2221 ctcgctcggt gtgatcgggg agtatctcgg ccgtgtctac gaggaggtga aggcgcggcc
     2281 gctctacatc gaggcggaga cggtcggcgc gctcggcgtg atcagcccgg tggccgacgg
     2341 cacggggcgc ccgcacacgt cctcgggctc cggcgccggc cagcgctgat gacgcgcggg
     2401 cccggccgtc atcccacagc acacattgca ggcttcctcg cctcgggcgg gctcgccttc
     2461 acggtggatg ccggcgtgct ccttctcgcg acccagattg gtgggctcga ccccttcatc
     2521 gcgcgcgcac tcggcatcag cgcagccatg attgccggct ggctggccca ccgctcgtgg
     2581 acgtttgcag tggcagcgcg tccgaccttc aaagagttcc tgcactacgc cgcgctgcag
     2641 gccggctcga tcgcctcgaa ttacgcgatc tacgcggcga tcctgctcct caggcccgca
     2701 acggagccgc tcgtcgcgct cttcgtttca tccgcggtcg cggcagtcat cgcctacgtc
     2761 ggcatgcggc tcggggtctt ccggcgcgca cggtgagttg atccagcagg accgcgccgc
     2821 actcgtgttc tcaattgggc cgcttcgcat cggccgcctc gaaatgcgcg agctgatcca
     2881 ccagggcctt cttgaaggcc ggccgatcga gagcgcgggc gacataggcc atgagcgccg
     2941 gatggctgcc gagttcgccc agattgcgcg gaacgcgcag cgcatcggcc atcatgatgt
     3001 cggcgacggt gaagcgcgat gcggcgagcc actcgcgtcc ggcgatcacg gtctcgagcc
     3061 gctcgaagcg ctgcttcatc cagccggcca acgggttgct tggcggtttt ccgagattga
     3121 tgaaccacca gggcacggtc accatctcga tcgaattgag gccagcaacg agccactgca
     3181 gcgtgtcggc ccgtcccttg ggatcgcggg gcatcagggc gtcgctcttc tccgcgatgt
     3241 gcaggaggcc ggccccgctc tcgaagatgc ggatgtcgcc atcctcgaga aacggcacct
     3301 gcccgaatgg ctgccgcgcg agatgctcgg gcccgcggtc ctggaacggt accgtcgcaa
     3361 catcgtagag gatgcccgcc tcctcgagtg cccagcggag gcggagatcg cgcacatggc
     3421 cgcgcggacc tttcggcacc cagtcgaatg tccagatggt caggcgttgg ggcatggcgg
     3481 ctcctccgcg atctcagggg gcgtgcatca tgcccgcggc ccgcctactt cgccatcacc
     3541 ttcttgattg ccgcggagac cgagtggcgg accttgtgcc agcctttcga gccttcgctc
     3601 gtcatccaat tcatctgcag cgagcggcgc tgaccctcga aggggtgatg gccgtgccag
     3661 gaggtgtccg agcgcttgaa gacgaggagg gtcccgttgt cgggcggcac ctcggcgacg
     3721 aaatcgtcga cattgtgtcc gttcttcagg atgcggagcc gcccgcccgg ctgctgccag
     3781 gtgtcgttga ggtagagaag aacggtgatg atcttatcgc ggctgtcggt gtggatctgg
     3841 ccgtccttcg cgcgcgtgta gccgcggagc gaatacatct tcggccgccc cgagagatcg
     3901 accgcgaact tctcggcgat ggccgcctcg aacgccggac cgtcgagctc gtcgatgacc
     3961 tccttgatcg ccatgctttc gctcaggctc tcgatcgggt agctgccgcc cgcggcaatc
     4021 ggtggatacg tcgcgttgat gcgacggacc atgtccggcg ccaggaaccc cggcacgatc
     4081 gtgtagtgat atggctccgt gttgagcgtg gcggccttga gcttggcgag atcgagatgc
     4141 gccatggcgt cctcacgtga agttgcgtcg aatctgggat gagagcgtcg tcacgcgggc
     4201 gaccgtcacg ccctctgcag cagtcccggg atcatcgctt gccggcgaag ccagcggcgc
     4261 tcgatctcgg cggcgatctc cttggcggcg tcgagcggag cgtagctcca agtctcgaac
     4321 gaagtcatgg cgggcgagag cggccgcgtc gcgccatagc gccgcagcgc gtcgtggaaa
     4381 cgggcaaatt tcgggctgac cgtcggcggc acgaacacgt agaccggccg gcccgtggca
     4441 cacgcttcgc ccgtcatgtt ggcgctgtcg ccggtgacga tcagctgatc cgcgtgcgca
     4501 aggaactgcg ggtaggggtt ctcgccttcg ccccgccaga ggatgcgcgg gcggccctcg
     4561 gtcgccttgc cgacgcagtc gaggaggcgc ttgtgcgtgc gccgcgaggg cgtgatcatg
     4621 aagcttgcgc cgagccgcgc gaacgtggcg agggaggccg cgaaggtgtc gtcgtcggcc
     4681 tccgtatact tgaaggcatc gctcttgccg ccgaggatca cggcaacgcg cggagagggc
     4741 agagcagcga tgtcggccgg ggtgcttgcg cgcagcttcg cgagccgcgc ctccgtaaag
     4801 ctgtgggcag gaacgagcgt ggtaagcaca ttcggccccc gccggcgatc gtgctcggga
     4861 acgcatatga gatcggcgat ggaggcgctc gtgcgcgggt cctgaagcac gacgcaatag
     4921 gtaccgacgc ccgatgcctt ccggagcgcg cgaagataag ggatcgcgag ccgcccggtc
     4981 gccagcgcca catcagggaa tggcggcgcg aaaggtgtgc cgggcgcacc aaacttttcg
     5041 gcgggcgcga ccggtcccca cggcgccatc agccgccaca cgccctgcgg ggacacagac
     5101 ttccattcgt agtcgagccc gagcgcgtcg gcgacgccgc gggcctggac gatcatgccg
     5161 atcttgtcat cggtgatgag ccagccgcgc cggccgcgga gtgccgagct cgccgtgtct
     5221 ggtcgctgca tcggacgggc ctcgtcgcca gaaggaagtc ggttgcgcgc gcgcaggctc
     5281 acgcgtaact caatgcgccg caggaactga gggctactcc cgatgcgtca cgatctcgat
     5341 gctaccgatt ggcgcattct gcgggagctg atggcggacg cacgcatttc gaacgtgacg
     5401 ctctcggctc gcgtcgggct gtcacccgcg ccgtgcctgc gccgtgtgcg cgcgctcgag
     5461 gaggcgggcg tcattcgcgg ctaccatgcc catatcaacg ggccgcgggc cggcttcgat
     5521 ctggtcgcct atgcgatggt gcgcctgcac gcccaagcag aggccgacct cagggctttc
     5581 gagaacctcg ttctcacctg gccgctggtg cgcgaggcgc tgatgctttc gggcgatgtc
     5641 gactacatgc tgaaatgtat cgcgccggat ctgccgacgt tccaggactt cgtgctgcgc
     5701 aagctgaccg cggcgccgaa cgtcgcaagc gtcaagaccg cgctcgccat ccgcgtggcg
     5761 aaggacgagc ccggcgtccc gctgatgttc agctgacggc cttgcccaaa ctcgggcgcg
     5821 gcacgagcga acgagcgagg ctccccgcca aaggccttgg cctttgggaa ccacttgaaa
     5881 gggtccagga tcttggcttg gcgaggtcat ctcgcgcaga ccgaaagcgc cccgcctggc
     5941 aggtagcgct tcccgttctg gcgacggctc agctcagttg aggcggccct tgaggtcgcg
     6001 gatcgcctgg tcgacgagcg gcgcgccggc agcggtagtg ccgaccttgg ccttcaggat
     6061 gcgctctgca gcaaccacgg ccgcatcgat tgccgcggca cgcacctcgg cgacagcctg
     6121 ctgctctgcc cggccgatct tttcctcggc gaggcgggcg cggcgctcga gcgattcctt
     6181 caacgacttc tctgcggcga ccttcatagc ggcggcctcg cgctcggcgt tggcgacgat
     6241 ggcctgcgcc tcgtcgtctg cgagtcgctg cttgcgcgtg tactcgtcaa gcagctcctg
     6301 cgcctgctcg cggagcttgc gggcctcgtc cagctctttg cggatcgcga cggcgcgctg
     6361 atcgagcgcg cggccgagcg ctgccggcac tttgaaccag gcgaggaggg cgaggaagcc
     6421 gcagaaggcg acggcgaccc agaactcggg attgtggatg tcgaaaaaca tgccaatcac
     6481 tccccggcgg cgggttggag ggcgctgcgc acctcgtcgg ccgtcgggtc gacaccgatg
     6541 agcttcctga cgagcggacc cgcggtatcg gcggcgatcg caccgatgct ggcggtcgcc
     6601 gccgacttca tggcggcgat acgcttctcc gtttccgcca tcttggcggc gaggctcgcc
     6661 tcgagctccg cgcgctgctt gtcggaagcc tgggcgacgc ggtcgcgcgc ctccttggcg
     6721 tggttctgcg ctcggccgcg agcgtcggcg atcgacttct cgtaggccgc cttggtgatc
     6781 ggcaggtagc cggactcctg gtgcagcctc gcctggcggc cggtatccga cagga
//
LOCUS       DAORNH010000251         6809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8286_length_6809_cov_3.073438, whole genome shotgun sequence.
ACCESSION   DAORNH010000251 DAORNH010000000
VERSION     DAORNH010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8286_length_6809_cov_3.073438"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK264_19810"
     CDS             complement(<1..383)
                     /locus_tag="PK264_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78151.1"
                     /translation="MATDGTPVTRAGPAATASANDRGARNPARRVPRSVVLVASALAT
                     GLLFGMVALNRPMGVPTAVMERDVAIRVFGLGTVEARVQSKIGFEVGATLTELAVDHG
                     DRVTDPHHAGGGRDHRMTVLSHRQP"
     gene            682..2727
                     /locus_tag="PK264_19815"
     CDS             682..2727
                     /locus_tag="PK264_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRD78152.1"
                     /translation="MKQIRVTCAHDCPCMCSLIATVDNGRLVRVAGDPEQPFTDGFAC
                     GKVNRDAELVYSPDRIKTPLRRSGTKGSGTFSPISWREALDEIETRFKAIMAKSGPLA
                     VLGYAYSAHQGQINRHSVNGLFHALGTSRLSAGTVCDSCCETAWDMTLGPVGSADPET
                     VADADLIISWGCDLKAVNVHFWQKAERRQKEGVKIIVIDPRRTRTAQSADWHIPIRIG
                     TDAALAIGLVHIMVRDGKCDEAYLARNTIGFEQWKREVVANFPPARVAEITGVPIEDI
                     ERLAAMYGAAPRSFIRIGEGMTRLARGGQALRAVATLPAVTGAYGRRGGGALLMTAAS
                     MDFNFAPIRKPSGPAATRQVNHSLLGEALLTLRDPPIEGLLICANNPAVTNPDAGKVR
                     QGLMREDLFTVVHDPVMTDTAKFADVVLPAATYLETEDFYRAYGSYYTQFAPAAIEPV
                     GEAWSNFRLAQELARRLGVTDAIFRMTPREIFPKFFEGAKGPVAKVDPKRLLEHKAIK
                     VAPEGEGQEFRTRSGKLEIYSADLAAAGVAPMPIWEPDPQEVADAARWPLRLLTTPGY
                     FQSHTAFAGNKFLREREGEPCCVLHPGEAQKRGLADGTKVRLFNSRGSIGLVLKISDE
                     VQPGVAHVPGQRPSGEAVSGTVNVLCSDRLSDIGAGATYQSTFLEVEAWGAARAAAE"
     gene            complement(2751..3962)
                     /locus_tag="PK264_19820"
     CDS             complement(2751..3962)
                     /locus_tag="PK264_19820"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRD78153.1"
                     /translation="MHVGRLEDDFRLRRRAMGPGARGERAGGGRANQHPAAIDRGVFH
                     RFLPSGWPSDEFFSARSCRSKRSVSVWASCACRSVLLKQMIADRRPVELVELRFRSLL
                     LSTASPDRDHTGRYGSQSVRIACPCLLQLIHTCTMTRHLTSSADTPIDAARESAGAAL
                     LRPLRPQRNLADEVVDRIAAEIRSGRLPPGTQLPTEAALMRAMSVSRTVVREAVAALR
                     ADGLVVTRQGLGAFVAAEAGRSAFRIAGRPEAGETAISEVLRVMELRLAVEVEAAVLA
                     ASRASAAQIAIVRERLRAIDKAIAAGETAVTEDFAFHCAIAEATGNPHFSDFLSFLGR
                     HVIPRQVVRANQGSREERKAYLEIIQRDHERIYKAIRTHDPRGARTAMRAHLSKSIER
                     YRTFAVAVRAR"
     gene            3957..4844
                     /locus_tag="PK264_19825"
     CDS             3957..4844
                     /locus_tag="PK264_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD78154.1"
                     /translation="MHPLGYPTVEAVVNMGKKLEAATSGRISIQMFPSMQLGGEKEMI
                     EQAQLGALQIARISVGPMGPLVPEFNVFNLPFVFRDVVHMEKVVDGEIGTELLKKLTD
                     HPTANLIGLCYMNAGVRNVYNSKKPITSLADLKGLKIRMMGNPLFVDTMNALGGNGVA
                     MGFDQLINAMQTGVVDGAENNEPSYDSGQHYRYAKFYSKTGHLMIPEILVFSKKSFMA
                     LSADDRALIMKLSKEAQLEQRKLWYAREEESIKKMIAAGAVINDIADKKAFQDAVRPV
                     WDKYGAAHKALIERINAVK"
     gene            4938..5330
                     /locus_tag="PK264_19830"
     CDS             4938..5330
                     /locus_tag="PK264_19830"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HRD78155.1"
                     /translation="MALSITAIYAGLLGLIAIVLAAMVGRARVATGVSLGDAGRPALL
                     VAMRRQANFVEYVPLFVVLLAIVEINGAPRWWLHTLGSIVVAARIIHPFGLRHDVMRT
                     IPRFLGTVGTFLPIVALSLTALWTAFLR"
     gene            5406..5927
                     /locus_tag="PK264_19835"
     CDS             5406..5927
                     /locus_tag="PK264_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD78156.1"
                     /translation="MKSAYFAAMDALHQACLFLAGLALVVITLIIPWGVFTRYVLNSA
                     SSWPEPMAILLMIWLSFLSAVICYREHLHIAVGWLPGLLPPAVKYALGFLIELAMLAT
                     NLFLLWWGIGLCKATWFQSIAEFPAVSVGVSYLPVPIGGAITALFVIERLLKGNFFPP
                     PPEIEHAAPVSSE"
     gene            5964..>6809
                     /locus_tag="PK264_19840"
     CDS             5964..>6809
                     /locus_tag="PK264_19840"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRD78157.1"
                     /translation="MDVLVLVGSFAVLCFLGMPVAYSLGLAAVIAAIWMDIPLEAIAL
                     KTSDGMDDFPLLAIPYFILAGAIMAEGGMAARIINLAKVFVGFIRGGLALVNILASTM
                     FGCISGSSVADTASIGSVMIPQMIKAGYPRLFAVNVTIAGSLQPLLIPPSHNMVIYSL
                     AAGGTVSVAHLFIGGIIPGLLLGLSLMGLCLFIARRDNFPKGEVIGLRDAGRIAYEAV
                     WGLITVIIILGGILTGKFTPTESAAIACIWAFLVTLLIYRDYKLRELPTLIGRVVRTT
                     GMVMIM"
BASE COUNT         1191 a         2302 c         2142 g         1174 t
ORIGIN      
        1 tggggctgac gatggctgag gaccgtcatc cggtgatcgc ggccgccgcc ggcgtggtgt
       61 gggtcggtga cacggtcgcc gtggtcgacc gcgagttctg tcagtgtcgc gccgacctcg
      121 aagccgatct tcgattggac gcgcgcctcg accgtgccga gaccgaacac gcggatggcg
      181 acgtcccgct ccatcaccgc cgtcggaaca cccatcggcc gattgagggc gaccatgccg
      241 aagagcagac cggtcgcgag cgccgacgcg accagaacga cactccgagg aacccggcgg
      301 gcgggattgc gcgcgccgcg atcattggcg cttgcggtcg cagccggacc ggcacgcgtc
      361 acaggcgttc catctgtcgc catattctgc tccctagtcg ccggtctggt cgacaccgcg
      421 accagcctcg caccgggccc gcccgcagcg ttgacgtcgc gcaagtcgtg cgcgagccag
      481 gattgtggca gcgccgatcg agcgcgcatg taactatgtc actgtcgacc tcgcgaccgg
      541 cccggagctt gcgtgaaagt ggcctgctcg ttccccgaga agtggccatt gagagaaatc
      601 cactttggca ggacggtgcc gcaatcgtgt gcgccgccac acccggtgca ctggagcagg
      661 aatacgaagc aggaaccggt catgaagcag atccgcgtga cgtgcgccca cgattgcccc
      721 tgcatgtgct cgctcatcgc gaccgtcgac aacggccgcc tcgtgcgcgt tgcaggcgat
      781 cccgagcagc cgttcaccga cggcttcgct tgcggcaagg tcaaccggga tgcggagctc
      841 gtctactcac cggaccgcat caagacgccg ctgcgccggt caggcaccaa gggctccggc
      901 acattctcgc cgatcagctg gcgagaggcg ctcgacgaga tcgagacgcg cttcaaggcg
      961 atcatggcga agagtggccc gctggcagtg ctcggctatg cctatagcgc acaccagggc
     1021 cagatcaatc gccactcggt gaacggcctc ttccacgcgc tcggcacgag ccgcctctcc
     1081 gccggcaccg tatgcgacag ctgctgcgag acggcctggg acatgacgct cggccccgtc
     1141 ggcagcgccg atccggagac ggttgcggac gccgatctga tcatctcgtg gggctgtgat
     1201 ctgaaggccg taaacgtcca cttctggcaa aaggccgagc ggcgccagaa ggagggcgtg
     1261 aagatcatcg tcatcgatcc gcggcgcacg cggacggcgc agagcgccga ctggcatatc
     1321 ccaatccgca tcggtaccga cgccgcactc gctatcggtc tcgtgcacat catggtgcgc
     1381 gacggaaaat gcgacgaggc ctacctcgcc cgcaacacga tcgggttcga gcagtggaag
     1441 cgcgaggtcg tcgctaactt cccgccggcg cgcgtcgccg agatcaccgg cgtgccgata
     1501 gaggacatcg agcgcctcgc ggcgatgtac ggcgcggcgc cccgctcctt catccggatc
     1561 ggggaaggca tgacgcgcct cgcccgcggc ggacaagcgc tccgagccgt cgccacgctt
     1621 cccgcggtga ccggcgccta tggccggcgc ggcggcggcg cgctgctcat gaccgccgcc
     1681 tcgatggact tcaacttcgc gccgatccgc aagccatcgg gaccggcggc gacgcgacag
     1741 gtcaatcaca gcctgctcgg cgaagcgctc ctcaccctcc gcgatccccc gatagagggg
     1801 ctgctcatct gcgccaacaa tccggcggtc accaatccgg acgccggcaa ggtgcgtcag
     1861 ggcctcatgc gagaggacct cttcaccgtc gtccacgatc ccgtaatgac cgacacggcc
     1921 aagttcgccg atgtcgtatt gccggcggcg acctacctcg agacggaaga cttctaccgc
     1981 gcctatggca gctactacac gcagttcgcc ccggctgcca tcgagccggt cggcgaggct
     2041 tggtcgaatt tccgtctcgc gcaggagctc gcgcgccgcc tcggcgtcac cgatgcgatc
     2101 tttcggatga cgccgcgcga aatctttccg aaattcttcg agggcgcgaa ggggccggtc
     2161 gccaaggtcg acccgaagcg gctgctcgag cacaaggcca tcaaggtggc gccggagggc
     2221 gagggccagg aattccgcac gcgctcgggc aagctcgaga tctattcggc cgatctcgcc
     2281 gcggcgggcg tcgcgccgat gccgatctgg gagcccgatc cacaggaggt cgccgatgca
     2341 gcgcgctggc cgctccggct gctgacgacg cctggctatt tccagtcgca cacggcattt
     2401 gcagggaaca agttcctgcg cgagcgcgag ggcgagccct gttgcgttct ccatccgggc
     2461 gaggcgcaga agcgtggcct cgccgacggc acgaaagtcc ggctcttcaa ttcgcgcggc
     2521 tcgatcggcc tcgtactcaa gatcagtgac gaggttcagc cgggagtcgc acacgtgccc
     2581 ggccagcgac cctcgggcga ggcggtctcg ggcaccgtga acgtgctctg ctcggatcgg
     2641 ctcagcgaca tcggtgcggg tgccacgtac cagagcacct tcctcgaggt cgaggcctgg
     2701 ggcgcggcgc gcgcggctgc ggaatagcgt cgagcatggc tgcgcgagct tcagcgcgcg
     2761 cgcacagcca ccgcgaatgt ccggtagcgt tcgatgctct tcgacaggtg cgcccgcatc
     2821 gccgtccgtg cgccgcgcgg atcatgcgtg cggatcgcct tgtagattcg ctcgtgatcg
     2881 cgctggatga tctcgagata ggccttgcgc tcctcgcgcg aaccctgatt ggcccgcacc
     2941 acctgacgcg ggatgacatg gcggccgagg aacgacagga aatcgctgaa atgcggattg
     3001 cccgttgcct cagcaatcgc acaatggaaa gcgaaatcct cggtcaccgc ggtctcgccc
     3061 gcggcaatgg ccttgtcgat ggcgcgcaac cgctcgcgaa cgatggcgat ctgggccgca
     3121 ctggcgcgcg aggcggcgag caccgccgcc tcgacctcga cggcgaggcg caactccatc
     3181 acgcgcagca cctcgctgat cgccgtctcg cccgcttccg gccgaccagc gatgcggaag
     3241 gccgacctgc cagcttccgc agcgacgaat gctccaagcc cctgacgcgt cacgacgaga
     3301 ccatccgctc gcagcgcggc gaccgcctcg cgaacgacgg tacgcgacac gctcatggcg
     3361 cgcatcagcg ccgcctcggt cggaagctga gtgccgggcg gtagccggcc cgatcggatc
     3421 tcggccgcga tgcggtccac cacctcatcg gcgaggttcc gctgtggccg cagcggccgc
     3481 agcagcgcgg caccagcgga ttcccgagcg gcgtcgatag gggtatcggc gcttgacgtc
     3541 agatgtctgg tcatcgtaca agtatgtatc agctgcaaca gacatggaca agcgatccgc
     3601 accgactgcg atccgtatcg tccggtatgg tcgcgatctg gagacgcagt gcttaggagc
     3661 agggatcgga acctcagttc aaccagttcc acaggtcgtc gatctgctat catttgcttc
     3721 aacaggacgc tgcgacaagc gcacgacgcc cacactgaaa ccgaccgttt cgaacgacaa
     3781 gaacgagcgc tgaaaaactc gtcactcggc caaccacttg ggagaaaacg atgaaacaca
     3841 cctcgatcga tcgccgccgg atgttgattg gcgcggccgc cgccagcgcg ctcgccgcga
     3901 gcgccgggac ccatcgcgcg gcggcgcagg cgaaagtcgt cctcaaggcg accgacgtgc
     3961 acccgctcgg ctatccgacg gtcgaggccg tcgtgaacat gggcaagaag ctcgaagcgg
     4021 caacgagcgg acgcatctcg atccagatgt tcccctcgat gcagcttggc ggcgagaagg
     4081 agatgatcga acaggcgcag ctcggcgccc tccagatcgc gcgcatctcg gtcggcccga
     4141 tgggcccgct ggtccccgag ttcaacgtct tcaacctgcc gttcgtgttc cgtgacgtcg
     4201 tccacatgga gaaagtcgtc gacggcgaga tcggcacgga actcctgaag aagctcacgg
     4261 accatccgac cgccaacctg atcggcctct gctacatgaa tgcgggcgtg cgcaacgtct
     4321 acaacagcaa gaagccgatc acgtcgctcg cggacctgaa aggcctcaag atccgcatga
     4381 tgggcaaccc gctgttcgtc gacacgatga acgcgctcgg cggcaatgga gtcgccatgg
     4441 gcttcgacca gctcatcaat gccatgcaga cgggcgtcgt cgacggcgcc gagaacaacg
     4501 agccgagcta cgattccggc cagcactacc gctatgcgaa gttctactcg aagaccgggc
     4561 acctcatgat cccggaaatc ctcgtcttct cgaagaagag cttcatggcg ctctcggccg
     4621 acgaccgcgc gctcatcatg aagctctcca aggaagcgca gctcgagcag cggaagctct
     4681 ggtacgcgcg cgaggaggaa tcgatcaaga agatgatcgc cgccggcgcc gtcatcaacg
     4741 acatcgccga caagaaggcc ttccaggatg cggtccgccc ggtatgggac aagtacggtg
     4801 cggcgcacaa ggcgttgatc gagcgcatca atgctgtgaa gtgaagtccg gaggaactgc
     4861 ggtggcccgc tcctctcgag ggggccatcg aggcaggccg gcacaattgg tcggtccgag
     4921 agattggaaa tgtccttatg gcattgtcga tcacggcaat ctacgccggc ctgcttggtc
     4981 tcatcgccat tgtgctcgcc gcgatggtcg gccgtgctcg ggtcgcgacc ggcgtatcgc
     5041 ttggcgacgc gggcaggccc gcgctgctcg tggccatgcg ccgacaggcg aacttcgtcg
     5101 agtatgtgcc cctgttcgtc gtgctgctgg cgatcgtcga gatcaacggc gcgccgcgct
     5161 ggtggctgca tacgctcgga tccatcgtcg tcgcggcacg catcatccat cctttcggac
     5221 ttcgccacga cgtcatgcgc acgataccgc gattcctggg cactgtcggc acgttcctgc
     5281 cgatcgtggc gctctctttg acggcactgt ggaccgcctt cctacgctga cgtggtggtc
     5341 cgggagctct tgctcgcgca ccggctgcct taggcacagc acttggccga tcgggtgata
     5401 gactgatgaa gtcagcctat tttgccgcca tggacgcgct ccatcaggcg tgcctgttcc
     5461 tggcgggcct cgcccttgtg gtgatcacgc tgatcatccc gtggggcgtc ttcacgcgct
     5521 atgtactcaa cagcgcttcg tcctggccgg agcccatggc gatcctgctc atgatctggc
     5581 tgtcgttcct ctcggcggtc atctgctatc gcgagcacct gcatatcgcg gtcggctggc
     5641 tgcccgggct gctgccgccc gcggtcaagt atgcgctcgg cttcctcatt gagcttgcta
     5701 tgctcgcgac caacctgttc ctactgtggt ggggcatcgg gctctgcaaa gcgacctggt
     5761 tccagtcgat cgccgaattc ccagcagtgt ccgtcggcgt atcgtaccta cccgtgccga
     5821 tcggtggagc catcacggca cttttcgtca tcgagcgact cttaaagggc aacttcttcc
     5881 cgccgccgcc ggagatcgag cacgcagccc ccgtctcgag cgaatagacc gcgccgcctg
     5941 aacacaaacc tgggacatga gcaatggacg tcctggtact cgtcggtagc tttgccgtgc
     6001 tctgctttct cggcatgccc gtggcctact cgctcggcct cgcagcggtg atcgccgcca
     6061 tctggatgga catcccgctc gaggcgatcg cgctcaagac gtccgacggc atggacgact
     6121 tcccgctgct cgccatcccc tatttcatcc tcgccggcgc catcatggcc gaaggcggca
     6181 tggccgcccg catcatcaac ctcgcgaaag tgttcgtcgg cttcatccgc ggcggacttg
     6241 cactcgtcaa catcctggcc tcgaccatgt tcggctgcat ttcgggttcg tctgtcgccg
     6301 acacggcctc gatcggctcg gtgatgatcc cgcagatgat caaggccggc tacccgcgcc
     6361 tgttcgccgt caacgtgacg atcgccggct cgcttcagcc cctgctgatc ccgccctcgc
     6421 acaacatggt gatctactcg ctcgccgcgg ggggcacggt atcggtcgct catctcttca
     6481 tcggcggaat cataccgggt ctgctgctcg gcctatcgtt gatgggcctc tgcctcttca
     6541 tcgcgcggcg cgacaacttt ccgaagggcg aggtgatcgg gctccgcgac gcgggccgca
     6601 tcgcctacga agcggtctgg ggcctgatca cggtcatcat catcctcggc ggaatcctga
     6661 cgggcaaatt cacaccgacc gaaagcgcgg cgatcgcctg catctgggcg ttcctcgtca
     6721 cactgctcat ctaccgcgac tacaagctcc gggagctgcc gacactcatc ggccgcgttg
     6781 tgcgcacgac cggcatggtg atgatcatg
//
LOCUS       DAORNH010000252         6758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8362_length_6758_cov_2.526331, whole genome shotgun sequence.
ACCESSION   DAORNH010000252 DAORNH010000000
VERSION     DAORNH010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8362_length_6758_cov_2.526331"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..905
                     /gene="lptB"
                     /locus_tag="PK264_19845"
     CDS             123..905
                     /gene="lptB"
                     /locus_tag="PK264_19845"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRD78158.1"
                     /translation="MPPPIPGAAPSAVYPASDVSGEGVLAAEGVKKAYRKRQVVKGVS
                     LNVRRGEAVGLLGPNGAGKTTVFYMITGLVPADAGRIYVDGRDVTPLPMYRRARLGIG
                     YLPQEASIFRGLSVEQNIMAVLELVEPNRRKRREQLDSLLEEFSITRLRKSPSIALSG
                     GERRRCEIARALASRPSFILLDEPFAGIDPIAVGDIQQLVRQLTTLNIGVLITDHNVR
                     ETLSLVDRGYVIADGQVLTQGKPSEIIANEDVRRVYLGNMFG"
     gene            965..2500
                     /gene="rpoN"
                     /locus_tag="PK264_19850"
     CDS             965..2500
                     /gene="rpoN"
                     /locus_tag="PK264_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRD78159.1"
                     /translation="MALATRLELRQGQQLVMTPQLQQAIRLLQLSNLELVQFVEAELE
                     KNPLLERDDDSGSARDVEPATEPAEANSSGDEAPDPPSLEPTFDGDAASACDTDFENA
                     YPDATRGDAATDPVQMLAGSSWSNVGPGGGDDDQSLEDYLSETPTLKDHLLGQMLLSS
                     LDPVQGLIAQHLLDQLNDVGYLDADIAEIAERLAAPVPLVESVLERLQKLDPPGVFAR
                     SLAECLALQLKEQRRYDPMIAKVLDNLPLLASHNLQALRRAVDCEMSELVEMISEIKR
                     LNPRPGAGFGASPVQTVVPDVIVRPGAHGSWIVELNTETLPRVLVNRSYFSLVSKTAR
                     HDKDKDYITECLQNANWLVKSLDQRARTILRVAEEIVRQQDGFFAHGVQHLRPLNLKT
                     VADAIKMHESTVSRVTSNKYMATSRGIFELKYFFTSAIAAAGEGDAHSSEAVRHRIRQ
                     LIDAETAANVLSDDRIVELLGREGIDIARRTVAKYREGMRIASSVQRRRQKKLAERTA
                     MAS"
     gene            complement(2524..>2890)
                     /locus_tag="PK264_19855"
     CDS             complement(2524..>2890)
                     /locus_tag="PK264_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78160.1"
                     /translation="TTFNGIEREKMAEATAVYHLIRNIGASLFISICVAEVVRSTGFN
                     YARMAEFVSPFNRALLAPVFEGVYDVSSARDLERLSREISKQAAMIAYINAFGLFTAA
                     AAIAMPLILLLKGIATRRV"
     assembly_gap    2891..2900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2901..>3276)
                     /locus_tag="PK264_19860"
     CDS             complement(<2901..>3276)
                     /locus_tag="PK264_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78161.1"
                     /translation="SHNPFLDLKLLGNRNYTLGLVLIALFGMLNFTPMVLLPTLMRGQ
                     MGFPDSLVGQVVGARGLGGLGGFFAVMFLTRLDPRISVAAGFGLLVIAGAWLMRMDLN
                     VTALELSLNGIVLRSDQTKAQA"
     assembly_gap    3277..3309
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3310..4074)
                     /locus_tag="PK264_19865"
     CDS             complement(<3310..4074)
                     /locus_tag="PK264_19865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78162.1"
                     /translation="MTESSDQSASEAARYPPLARRVLILTSTVLTVALYFTSILVAST
                     VLPQMQGSFAATADEISWTMTFNILATAIAMPTTGWLVARYGRRRLMVWSTGLFTLST
                     LMCGLAQSLEIMIVWRIVQGAAGAPSVPLVQTIILDTFPPRQHRVVLGIYGMGVVLGP
                     ILGPTLGGYLADVMNWRWAFFLLVPVGAVATLGLAFTLPADRSTGPARFSWPGFIFLS
                     VAIGGLQWVLARGQRLDWLESFEIQASIVIAAVSFYL"
     gene            4276..4677
                     /locus_tag="PK264_19870"
     CDS             4276..4677
                     /locus_tag="PK264_19870"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRD78163.1"
                     /translation="MFSVEVRDRIMIAHSLPDPFFGPAAKKHGATFVVDVAFFREKLT
                     RHNVVVDIGAALDVLNKTLKPLAYKDLDEIADFKGKLTTTEFLCKHIFDAIAKAARAG
                     ALGDDGKGLAKIRVTLHETDLARAAYEGPLG"
     gene            complement(4687..5253)
                     /locus_tag="PK264_19875"
     CDS             complement(4687..5253)
                     /locus_tag="PK264_19875"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HRD78164.1"
                     /translation="MNAPLSLTMTKAEFNLWIGRQATKHEWKEGRVVQMTNVTKAHAR
                     IVANLIRALSRQFDPVEWSVTASDLGVEGDNFIRYPDVIVERMDDDDKGRRARQPIVL
                     VEVLSVSTEATDLVEKLGEYRAFASLEAYVVASQDTPKLWVWQRDAAGEFATEPALVE
                     GRGAMLGLSARGISVPLAELFLGVAAGT"
     gene            complement(5286..6710)
                     /locus_tag="PK264_19880"
     CDS             complement(5286..6710)
                     /locus_tag="PK264_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRD78165.1"
                     /translation="MTFVDLELSETVLRAVTDAGYTKPTPIQEQAIPIILMGRDVLGC
                     AQTGTGKTASFTLPMIDILAAGRAKARMPRSLILEPTRELAAQVAENFDMYGKYNKLS
                     KALLIGGESMAEQIKVLERGADVLIATPGRLLDVFDRGRLLLNDVRILVIDEADRMLD
                     MGFIPDVERIVSLVSRMRQTLLFSATMPPAIRRLADAFLTNPKEITVAPPSTPAETVT
                     QGLVIVPHGGQGGLKEKRAALRTLIRSEDVRTALIFCNRKRDVGVLHRSLLRHGFDAG
                     LLHGDLAQSQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRSFCLPAM
                     ENVALWHERDISHSSVERMIGPDATVTLDFALNRLAGVIENLVVYPENMRRNLDRLGG
                     LIHSQRVLLALTQAGVSREEAYALVQRNAMPVWRGEGDFLTLLKADKDVAKTLKTKDL
                     EALFDLGYHLKHVDTIFARVFRTG"
BASE COUNT         1222 a         2200 c         2228 g         1065 t
ORIGIN      
        1 caccgttccc gagtggcggc ggcaactgaa ccctcatgcg tcagccggat tgaccgcaca
       61 tgaagctctt cgcccgttac ctgaagcgcg cctcgccgga tgccgctcgc gccgtccggc
      121 cgatgccgcc gccgatcccg ggggcggcac cgtcagccgt ttacccggcc tccgacgtga
      181 gcggcgaagg cgtgctcgcc gcggagggcg tgaagaaggc ctatcgcaag cgccaggtcg
      241 tcaagggtgt gagcctcaat gtccgccgcg gtgaggccgt gggcctcctt ggcccgaacg
      301 gagccggcaa gaccaccgta ttctacatga tcacgggcct cgttccggcc gacgccggcc
      361 gcatctacgt cgatggccgg gacgtgacgc cattgccgat gtaccggcgc gcgcgcctcg
      421 gtatcggcta cctgccgcag gaggcttcga tcttccgcgg cctcagcgtc gagcagaaca
      481 tcatggccgt gctcgagctc gtcgagccca atcggcgcaa gcggcgcgag cagctcgaca
      541 gcctgcttga ggagttctcg atcacgcggc tgcgcaagag cccgtcgatc gccttgtcgg
      601 gtggagagcg gcgacgctgc gagattgccc gcgcgctggc ctcccgcccg tcgttcatcc
      661 tcctcgacga gccctttgcc gggatcgacc cgatcgccgt cggcgacatc cagcagcttg
      721 tccggcaatt gaccacgctc aacatcggtg ttctcatcac cgatcacaac gtgcgcgaga
      781 cgctgagcct cgtcgatcgg ggctacgtga tcgccgatgg tcaagttctg acacaaggta
      841 agccttcgga aatcatagcc aatgaagacg tgcgccgtgt ctacctcggg aacatgttcg
      901 gttaaagcgc cgcaagtata gtccgctgca ggacgaccgc gctcgtgcgg gctaggggaa
      961 ggcaatggcg ctcgcaacgc ggctcgagct gagacaaggc cagcagctgg tgatgacacc
     1021 ccagctgcag caggccatcc gtctgctcca gctctcgaac ctggagctcg tccagttcgt
     1081 cgaggccgag ctcgagaaga acccgctgct cgagcgggac gacgatagtg gcagtgcgcg
     1141 cgacgtcgag cccgccacgg agcctgccga ggccaacagc agcggcgacg aggcgcccga
     1201 tcccccttcg ctcgagccga ccttcgacgg cgatgccgcg agcgcctgcg ataccgattt
     1261 cgagaacgcc tatcctgacg cgacccgcgg agacgccgcg accgacccag tgcagatgct
     1321 cgcgggatcg tcgtggtcca acgtcggtcc gggcggcggc gacgatgatc agagcctcga
     1381 ggattatctc tcggagacgc cgacgctgaa ggatcacctg ctcggccaga tgctcctctc
     1441 ctcgctcgat ccggtgcagg gcctgatcgc gcagcatctt ctcgaccagc tgaatgacgt
     1501 cggctatctc gacgcggaca ttgcggagat cgcggagcgt ctggcggctc ccgtgccgct
     1561 cgtcgagagc gtgctcgagc ggctccagaa gctcgatccg ccgggcgtct tcgcgcgctc
     1621 gctcgcagag tgcctggcac tccagctcaa ggagcagcgg cgctacgacc cgatgatagc
     1681 gaaggtgctc gacaatctgc cgctgcttgc gagccacaat ctccaagcgc tgcgacgtgc
     1741 cgtcgactgc gagatgtccg agctcgtcga gatgatcagc gagatcaagc ggctcaaccc
     1801 gcggcccggc gccggcttcg gcgcgagccc ggtgcagacg gtggtgcccg acgtcatcgt
     1861 gcggccgggt gcgcacggta gctggatcgt agagctcaac accgagacgc tgccccgcgt
     1921 tctcgtcaac cgcagctact tctcgctcgt ctccaagacg gcgcgtcacg acaaggacaa
     1981 ggactacatc acggagtgcc tgcagaacgc caactggctg gtgaagagcc tcgaccagcg
     2041 cgcgcgcacg atactaaggg tcgccgagga gatcgtgcgc cagcaggacg gcttcttcgc
     2101 gcacggcgtc cagcatctcc gcccgctcaa cctgaagacg gtcgccgacg ccatcaagat
     2161 gcacgagtcg accgtcagcc gcgtcacgtc gaacaagtac atggcgacga gccgcggcat
     2221 cttcgaactc aagtacttct tcacatccgc cattgccgcc gccggcgagg gtgatgcgca
     2281 ttcgtccgag gccgtgcgcc atcgcatccg ccagctgatc gacgcggaga cggccgccaa
     2341 cgtgctctcc gatgatcgca tcgtcgagtt gctcggccgc gagggcatcg acatcgcacg
     2401 tcgtacggtc gccaagtacc gcgaggggat gcgcatcgcc tcctccgtgc agcgccgccg
     2461 gcagaagaag ctcgccgaac gcacggccat ggcgagctga catccgcagg tgatcgcgac
     2521 cactcagacg cggcgcgtcg cgataccctt caagagcagg atcagcggca tggcgatggc
     2581 cgccgccgcc gtgaataggc cgaaggcgtt gatgtaggca atcatggcgg cctgcttgga
     2641 aatctcgcgc gagaggcgct cgagatcgcg cgcgctcgag acatcgtaga cgccctcgaa
     2701 cacgggcgcc aggagcgcgc ggttgaacgg cgagacgaac tcggccatgc gcgcgtagtt
     2761 gaatccggtc gagcgcacca cctcggcgac gcaaatcgag atgaagaggc tcgcgccgat
     2821 attgcggatg agatgataga ccgcggtcgc ctccgccatc ttctcgcgct cgatgccgtt
     2881 gaaggtcgtg nnnnnnnnnn tcggcctgcg ccttggtctg gtccgaacgc agcacgatgc
     2941 cgttgagcga gagctcgagg gcggtcacgt tgaggtccat gcgcatcagc caggcgccgg
     3001 cgatcacgag gaggccgaac cccgcggcga cactgatgcg cgggtcgaga cgggtgagga
     3061 acatcaccgc gaaaaagccg ccgaggccgc cgagcccgcg cgcgccgacg acctggccga
     3121 cgagactgtc gggaaagccc atctggcccc gcatgagcgt cggcagcaac accatgggcg
     3181 tgaagttcag catgccaaag agggcgataa ggacgagccc aagcgtatag ttgcggttgc
     3241 cgaggagctt gagatcgagg aacgggttgt ggctttnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnc aggtagaagg agaccgccgc gatcacaatg ctggcctgga tctcgaagga
     3361 ctcgagccag tcgaggcgct ggccgcgtgc gagcacccac tgcagtccgc cgatggcgac
     3421 cgacaggaag atgaaccccg gccagctgaa acgcgccggg ccggtcgagc ggtcggctgg
     3481 cagggtgaag gccaggccga gcgtcgcaac cgcaccgaca ggtacgagca ggaagaaggc
     3541 ccaccgccag ttcattacgt cggcgaggta gccgccgagc gtcggcccga ggatcggacc
     3601 gagcacgacg cccatgccgt agatgccgag cacgacgcga tgctggcgcg gcggaaaggt
     3661 gtcgaggatg atcgtctgca cgagtggcac actcggcgcg ccggcagcac cctgcacgat
     3721 ccgccagacg atcatgatct cgagcgactg ggcgagcccg cacatcaaag tcgacagtgt
     3781 gaacaggccg gtggaccaga ccatcaaccg acgtcgccca tagcgcgcga cgagccagcc
     3841 cgtcgtcggc atggcgattg ccgtcgccag gatgttgaag gtcatcgtcc aggagatttc
     3901 gtccgccgtt gccgcgaacg agccctgcat ctgcggcagc acggtggagg cgaccaggat
     3961 cgacgtgaaa tagagggcga cggtcagcac ggtggaggtc aggatcagga cgcggcgcgc
     4021 cagcggcgga taccgcgcgg cctcgcttgc cgactgatcg ctgctctccg tcatgtctca
     4081 tgccgattgg cggcgccgcg accggcggcg gctcactggg gcaccagcaa agcacgccgc
     4141 tcgactgggc gccaacccgc tgctgttgac gaccttacca ccgctgcagg tggcgcaagc
     4201 cgatgtcgat cagcgccgca ttgacggcgc gctcccggca ggcgatcagg gcacgagaca
     4261 gagagggaga cgaccatgtt ctcagtcgag gttcgcgacc gcatcatgat cgcgcattcg
     4321 ctgccggacc cgttcttcgg gccggccgcc aagaagcatg gggcgacgtt cgtggtcgac
     4381 gtcgccttct tccgtgagaa gctcacgcgc cacaatgtcg ttgtcgatat tggcgcggct
     4441 ctcgacgtgc tcaacaagac gctgaagccg ctcgcttaca aggacctcga cgagatcgcc
     4501 gacttcaagg gcaagctcac caccaccgag tttctgtgca agcacatctt cgacgcgatc
     4561 gccaaggccg cgcgggccgg cgcgctcggc gacgacggca agggcctcgc gaagatccgc
     4621 gtgacgctgc acgagaccga tctcgctcgc gcggcctatg agggcccgct cgggtaatcc
     4681 ggtggatcag gtgcctgcgg caacgccgag aaagagctcg gcgagcggca cggagatccc
     4741 gcgcgccgag aggccgagca tcgcgccgcg cccctcgacg agcgccggct cagtcgcgaa
     4801 ctcgcccgcc gcgtcgcgct gccagaccca gagcttcggc gtatcctggc tggcaacgac
     4861 gtaggcctct agcgaggcaa acgcgcggta ctcgccgagc ttctcaacga ggtcggtcgc
     4921 ctcggtcgag accgacagga cctcgaccag cacgatcggc tgacgcgcgc ggcgcccctt
     4981 gtcgtcgtca tccatgcgct cgacgatgac gtccggatag cggatgaagt tgtcgccttc
     5041 gacgccgaga tcggaggccg tgaccgacca ctctaccgga tcgaactggc ggctcagcgc
     5101 ccgaataagg tttgcaacga tccgcgcgtg cgccttggtc acattcgtca tctgcacgac
     5161 gcggccctcc ttccactcgt gcttggttgc ctgccggccg atccagagat tgaactcggc
     5221 cttggtcatt gtgagcgaga gaggggcgtt catgcgatgc ggtccttccc gtcgatcgcg
     5281 gtgcgtcagc ccgtcctgaa cacgcgggca aagatcgtat cgacgtgctt cagatgatag
     5341 ccgaggtcga acagcgcctc cagatccttt gttttcaagg tcttggcgac atccttgtcg
     5401 gccttgagga gggtgaggaa atcaccctcg ccgcgccaga cgggcatcgc attgcgctgc
     5461 acgagcgcat aggcctcctc gcgcgagacg ccggcctgtg tcagggccaa cagcacgcgc
     5521 tgcgagtgga tgaggccgcc gagccggtcg agattgcggc gcatgttctc cgggtagacg
     5581 acgagattct cgatgacacc ggcaaggcgg ttcagggcga aatcgagcgt cacggtcgcg
     5641 tcggggccga tcatgcgctc gacggaggag tgcgagatgt cgcgctcgtg ccagagcgcc
     5701 acgttctcca tcgcgggcag gcagaaactc cgcaccatgc gggcgagccc ggtgatgttc
     5761 tcagtgagca ccgggttgcg cttgtgcggc atggcggacg agcccttctg gccctccgag
     5821 aagaactcct ccgcctcgag cacctcggtg cgctgcgatt gcgccagatc accgtgcagc
     5881 agcccggcgt caaagccgtg acggagcaag gaacggtgca gcacgccgac gtcgcgcttg
     5941 cgattgcaga agatcagcgc ggtgcgcacg tcctcgctgc ggatcaatgt gcgcagggcc
     6001 gcccgcttct ccttcagccc gccctgcccg ccgtgcggga cgatcaccag gccttgcgtg
     6061 accgtctccg ccggggtcga cggcggggcg acggtgattt ccttcgggtt ggtgaggaag
     6121 gcgtcggcga gccgccggat cgcgggcggc atcgtcgccg agaacagcag ggtctgccgc
     6181 atgcgcgaga ccagcgagac gattcgctcg acgtcgggaa tgaagcccat gtcgagcatc
     6241 cgatcggcct cgtcgatcac caggatgcgc acgtcgttga gcagcagccg tccgcgatcg
     6301 aacacgtcga gcagccggcc aggggtggcg atcagcacat cggcaccgcg ctccagcacc
     6361 ttgatctgtt cggccatgga ttcgccgccg atcagcaagg ctttgctgag cttgttgtac
     6421 ttgccgtaca tatcgaagtt ctcggccacc tgtgctgcga gctcgcgggt gggttcgaga
     6481 atgagcgagc gcggcatccg cgctttcgcc cggccggcgg caaggatgtc gatcatcggc
     6541 agggtgaacg aggcggtctt gccagtgccg gtctgcgcgc aaccgagcac gtcgcggccc
     6601 atcaggatga tgggaatcgc ctgttcttgg atcggtgtag gcttggtgta gcccgcgtcg
     6661 gtaacggcgc ggagcaccgt ttcgctgagc tccaggtcga cgaaagtcat tgagagcggt
     6721 ccgtgcgccg tctagcttgc agcgcgatcg acgaaagc
//
LOCUS       DAORNH010000253         6744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8387_length_6744_cov_2.615189, whole genome shotgun sequence.
ACCESSION   DAORNH010000253 DAORNH010000000
VERSION     DAORNH010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8387_length_6744_cov_2.615189"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK264_19885"
     CDS             complement(<1..563)
                     /locus_tag="PK264_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HRD78166.1"
                     /translation="MPIEMPDVRPAGPLAIARALTLEGFANAVIAVLFAAAGPIAIIL
                     SAGKGRLTEAELASFVFGAFFVNGLLTILFSALYRLPLAFFWTIPGTVLIGPALQSWS
                     YPEIVGAYMATGILMFVLGLTGLVRRVMTAVPMPVVMAMVAGVFLPFGLDLVRAFERN
                     IAIAAPMTLAFLVLSAVPALGRLLPPLL"
     gene            complement(703..1209)
                     /locus_tag="PK264_19890"
     CDS             complement(703..1209)
                     /locus_tag="PK264_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78167.1"
                     /translation="MRHSAAADGRVEFSNDAPQASGSIGLQATSMVLFGLAAPVILFS
                     LVDPNLLRHGKLVVLALLMPALFVAVGVYVYSVLVPGDVASIVVDPQLRQIELSRNGS
                     FAIRSEIIPFDEVERVLLARHYDDDGYGVVEGQLRLRSGATIAISGFSSEDTIKALAR
                     AIGVRADS"
     assembly_gap    1340..1362
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1363..2082)
                     /gene="ubiG"
                     /locus_tag="PK264_19895"
     CDS             complement(<1363..2082)
                     /gene="ubiG"
                     /locus_tag="PK264_19895"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HRD78168.1"
                     /translation="MTEARSSSAATLDREEVRQFDRIAAEWWDANGKFRPLHQIGPAR
                     IEYARDRIAEHFGRTIKGMRPLAGLSLVDIGCGGGLVAEPMSRLGAEVTGLDPSAETI
                     RAAERHAAAGGLPIAYRQGLAEDLAAEGARFDVVLCLEVVEHVPDVGQFVKLLSGLLA
                     PGGLMVLSTINRTVKAYALAIVGAEYVLRWLPVGTHKWERFVTPDELARHLEVAGLAM
                     SEPTGVVYSPLTDTWRLDRDTD"
     gene            2290..2883
                     /locus_tag="PK264_19900"
     CDS             2290..2883
                     /locus_tag="PK264_19900"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HRD78169.1"
                     /translation="MARTATLLGVAAVLLAGLPVMAGKGGPADIARRLAKAREVATDA
                     GDRLRSEVNQILRSSGPLAGIGACQAAMPSIVQSAAENSGFELTRTALKLRNPEHAPD
                     AWELKTLELFVARIAAGADVGKLEHHEVVVTSEGERQFRYMKAIPMGEPCLTCHGPDI
                     RQDVRLEIARTYHNDKAVGFKLGELRGAFSLSQFIDE"
     gene            complement(2905..3948)
                     /gene="speB"
                     /locus_tag="PK264_19905"
     CDS             complement(2905..3948)
                     /gene="speB"
                     /locus_tag="PK264_19905"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HRD78170.1"
                     /translation="MDPGKLERLREKYAEAKGGDISDPHFAAVARMVFSEPDRRRWPF
                     AEPGTLLGAPYIRDWHLAVQSHPLDVALVGVPMDLGVTNRAGARLGPRAVRGVERIGP
                     YEHVLRVAPMGALKVADIGDVPMRSRYSLADCHADIEGHFRSIRAAGVLPLAVGGDHS
                     VSLPILKALGDGRPVGMVHIDAHCDTGGAYEGTKFHHGGPFRQAVLEGVLDPERTIQI
                     GIRGGAEYLWEFSYESGMTVLHTEDVMRLGLDAVIARARAIVGAGPTYVSFDVDSLDP
                     AFAPGTGTPEVGGLTPREALAILRGLAGIDIIGGDVVEVAPQYDPTTVTAQCGAQMLF
                     ELLCLVALRPQPR"
     gene            complement(3953..5113)
                     /gene="argE"
                     /locus_tag="PK264_19910"
     CDS             complement(3953..5113)
                     /gene="argE"
                     /locus_tag="PK264_19910"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HRD78171.1"
                     /translation="MAASIHTAKEILGRLVSFDTTSHKSNVPCAEWIRDFLASEGVEA
                     RLLPSPDGNWANLFATVGPAGNGGIALSGHMDVVPVTGQPWETDPYTMVEKDGRLYGR
                     GTCDMKGFLACVLATVPLYKRRRLKTPLHIAFSYDEEVGCTGVKPMIAEFGKSLPKPG
                     VVFVGEPSLMSVVDAHKGGYRYRTEITGKDAHSSKPQLGVGAIMVAGELIAELKRIEQ
                     RLGERFSNPRFDPPYPSISVSMIEGGIAHNIIPPKCTFHWGVRAMPGCSSAEIRAELM
                     AFAERELLPEMRKVHPDCAITTKLVGELPPFDSGRDSKATSLALKLVGQNATFAVPYG
                     TEASHFQNAGCSSVICGPGSIDQAHQPNEFVAIAELERCLAFLDRVGALAEG"
     assembly_gap    5264..5304
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5305..>6744
                     /locus_tag="PK264_19915"
     CDS             <5305..>6744
                     /locus_tag="PK264_19915"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRD78172.1"
                     /translation="RRMTGGQALAEMLKLADVGPMFGMGGFQLLPFYEACRALKLQHY
                     LINDERSGAFAADAYAKVTNRPGVVDATLGPGATNLLTGLVESLNAGLPMIAIVGDAN
                     REHAWKNMTQETRQIEMLRPAVKEVIRVEVIKRLPEHVRRAFAVATSGRPGPVIIDVP
                     EDIAHGEHDFDAADFWIDPGTLKAQARRLRADPAELARAVALLAKAERPIIMAGGGVH
                     ISDAHAALQTLAERHGIPVAHTMTGKGAIACTHALSAGLFGRYDRIANGMIAESDCLI
                     VVGCKLGEIATKRFQLIPPGKPVIHVEIVPDEIGRTTRTDIGLVGDARLVIEDITAAL
                     GDGKAAAGRRAQWCAEVPKRMAAWRKSAAERVESKESPINVGRLMGELNKIMPADGIV
                     VADGGFAAHWGGLFFDTKKAGRHFLPDRGLASIGYGVPGGIGAQIAAGKRRVVSLTGD
                     GGFNMSLGELETARRVGANFVAIVFNNAA"
BASE COUNT         1180 a         2256 c         2230 g         1014 t
ORIGIN      
        1 agcagcggcg gcagcaagcg cccgagcgca ggcacggcgg agagcacgag gaaggcaagt
       61 gtcatcggcg ccgcaatggc gatgttgcgc tcgaaggcgc gcacgagatc gagcccgaac
      121 ggcaggaaca cgcccgccac catggccatg acgaccggca tcggcacggc ggtcatgacg
      181 cgccggacga ggcccgtcag gccgagcacg aacatgagaa tgccggtcgc catataggct
      241 ccgacgatct cggggtagct ccagctctgc agcgcaggcc cgatcagcac cgtgcccgga
      301 atggtccaga agaaggcgag cggcagacgg tagagcgccg agaacaggat cgtgagcagg
      361 ccgttcacga aaaaggcgcc gaacacgaac gaggcgagtt ccgcttccgt gaggcgcccc
      421 ttgccggcgg acaaaatgat ggcgatcgga cccgccgcgg cgaacaggac ggcaatcacg
      481 gcgttggcga aaccctcgag cgtcagtgcg cgggctatag ccagcggccc ggcgggtcgc
      541 acgtctggca tctcgatcgg catcgcccgc cctcattcgc cggtcccgac agcgggcacg
      601 acagcgcccg cgcgacgagc gacgcgtaac gtagccgatc gcggaaagat agcctcgaga
      661 tcgcccagct cgggatcgga tgggcagcct tgcccgaccg cctcagctgt cggcacggac
      721 accgatggcg cgagcaagcg ccttgatcgt atcctcggag gagaagccgc tgatggcgat
      781 cgttgcaccc gagcgcagcc tgagctgccc ctcgacgacg ccgtagccat catcgtcgta
      841 gtggcgggcg agcagcacgc gctcgacctc gtcgaatggg atgatctcgg agcggatcgc
      901 aaacgagccg ttgcgactga gctcaatctg gcgaagctgc gggtcgacca cgatcgaggc
      961 cacgtcgccg gggacgagca cgctgtagac atagacgcca acggcaacga acagcgctgg
     1021 catcagcagc gcgaggacca cgagcttgcc gtgccggagc agattgggat cgaccagcga
     1081 gaacaggatc acgggcgccg cgaggccgaa cagcaccatc gacgtcgcct ggaggccgat
     1141 cgacccagac gcctgcggtg catcgttcga gaattcgact cggccatcag cggccgctga
     1201 gtgtcgcatg accgtctcca tacgcggata cgagcaattg taggggagct gagtttaatt
     1261 tccgtgcact gccaacatcc gatttcagca cgtcccgcaa ggctgtgtta acgcgtgctc
     1321 ggccgccgcg gcaggcgggn nnnnnnnnnn nnnnnnnnnn nnatcggtgt cacggtcgag
     1381 ccgccaggta tccgtgagcg ggctgtagac aacgccggtc ggctccgaca tcgcaaggcc
     1441 cgcgacctcc aggtggcgcg caagctcgtc gggggtgaca aaccgctccc acttgtgggt
     1501 cccgaccggc agccagcgca gaacatattc ggcgccgacg atggccaagg cgtaggcctt
     1561 caccgtgcga ttgatagtcg agagcaccat caggccgccg ggcgcaagca gcccactcag
     1621 cagcttcacg aactgaccga cgtcgggcac atgttcgacc acctcgaggc agaggacgac
     1681 atcgaaccgc gccccctcgg cggcgaggtc ctcggccagc ccctggcggt aggcgatcgg
     1741 taggcctccc gccgcggcat gccgctcggc ggcccggatc gtctcggcgg agggatccaa
     1801 ccctgtgacc tccgcgccga gccgcgacat gggctcggca accaggccgc cgccacagcc
     1861 gatgtcgacg agggagagcc cggccaacgg ccgcatgcct ttgatagtgc gcccgaaatg
     1921 ctcggcaatc cggtcgcgtg catactcgat ccgggccggg ccgatctgat gcagcggccg
     1981 gaacttgccg ttcgcatccc accattcggc ggcgatgcgg tcgaactgcc ggacctcctc
     2041 gcggtccaat gtggcagcgg agcttgagcg tgcttctgtc atgtaacggg tgtggggtcg
     2101 gcacctgtcg actgtcaata cacccgcacc aggcaaacta cggctgcgcc gaacagattt
     2161 gatattcgct cagattgaat tgccaacttg cgcgaccgat gacattgtcc cgccaccggt
     2221 cgggcgatat tcaggggcgc ccagaatgac agaagccggg cagacgtcga gggagaagtg
     2281 gacatgggca tggcgagaac cgccacgctg cttggcgtgg ctgcggttct gttggcaggc
     2341 cttcccgtca tggcaggcaa aggcggcccg gccgatatcg cgcggcggct tgcgaaggcg
     2401 cgtgaggttg ccaccgacgc gggcgatcgg ctgcgctcgg aggtgaacca gattttgcgc
     2461 tcgagcggac cactggccgg gatcggcgcc tgccaggccg ccatgccgag catcgtgcag
     2521 agcgccgccg agaattcagg cttcgagttg acgcgcacgg ccctcaagct gcgcaatccc
     2581 gagcatgcgc cggacgcctg ggagctgaag acgctcgagc ttttcgttgc gcgcattgct
     2641 gctggcgccg acgtcggcaa gctcgagcac cacgaggtgg tggtcacctc ggagggcgaa
     2701 cgccagttcc gctacatgaa ggccatcccg atgggtgagc cgtgtctcac ctgtcatggc
     2761 cccgatatcc gccaggacgt gcgcctcgag atcgcccgca cctaccacaa cgacaaggcc
     2821 gtcggcttca agctcggcga actgcgcggc gccttctcgc tctcgcagtt catcgacgag
     2881 tgatctggcc ggccacgagt gatgtcagcg cggttggggc cgcaatgcca cgaggcatag
     2941 cagctcgaac agcatctgcg ccccgcactg ggctgtgacc gtggtcgggt cgtactgcgg
     3001 cgccacctcg accacgtcgc caccgatgat gtcgatcccg gcgaggccgc gcaggatcgc
     3061 cagcgcctcg cggggcgtga ggccgccgac ctcgggcgtg cccgtgcccg gagcgaaagc
     3121 cgggtcgaga ctgtcgacat cgaaggacac gtaggtcggt ccggcgccga cgatggcccg
     3181 cgcccgcgcg atcacggcat cgagcccaag gcgcatcacg tcctcggtgt gcagcaccgt
     3241 catgccggac tcgtaggaga actcccagag gtactcggca ccgccccgga tgccgatctg
     3301 gatcgtgcgc tcgggatcga gcacgccctc gagcaccgcc tggcggaacg gaccgccgtg
     3361 atggaacttc gtcccctcgt aggccccgcc cgtatcgcag tgggcgtcga tatgcaccat
     3421 gccgacgggc cggccgtcac cgagcgcctt caggatcggc aggctcaccg agtgatcgcc
     3481 gccgacggcg agcgggagga cgccggccgc gcggatcgag cggaaatgcc cctcgatatc
     3541 ggcgtggcaa tcggcgagcg agtagcggct ccgcatcggc acgtcgccga tgtcggcgac
     3601 cttgagcgct cccatcggcg caactcgtag cacgtgctca tacggaccga tgcgctcgac
     3661 gccgcgcact gctctcggac caagccgcgc gccggcccgg ttcgttacgc cgaggtccat
     3721 cggcacgccg acgagggcga catcgagcgg atgtgactgc actgccagat gccaatcgcg
     3781 gatgtagggc gcgccgagca gcgtgcccgg ctccgcgaac ggccagcggc gccggtccgg
     3841 ctcggagaag accatccgcg ccaccgccgc gaaatgcggg tcggagatgt cgccgccctt
     3901 ggcttcggca tacttttcgc gaagccgctc gagcttgccc ggatccatgg catcacccct
     3961 ctgcgagcgc gcccacgcga tcgaggaacg cgaggcagcg ctcgagctcg gcaattgcca
     4021 cgaactcgtt cggctggtgt gcctggtcga tggagcccgg accgcagatc accgacgagc
     4081 agcccgcgtt ctggaagtgg ctcgcctcgg ttccgtacgg caccgcgaac gtcgcgttct
     4141 gtccgacgag cttcagggcg agggaggtcg ccttgctgtc cctgccggaa tcgaagggcg
     4201 gcagctcgcc gacgagcttg gtcgtgatcg cgcagtctgg atgcaccttg cgcatttcgg
     4261 gcagcagctc gcgctcggcg aaggccatga gctcggccct tatttcggcc gaagagcacc
     4321 ccggcatggc gcgcacaccc caatggaatg tgcacttcgg cgggatgatg ttgtgggcaa
     4381 tcccgccctc gatcatgctg acgctgatcg acggatatgg cggatcgaaa cgcgggttcg
     4441 agaagcgctc gccgagccgt tgctcgatgc gcttcagctc cgcaatgagc tcgcccgcga
     4501 ccatgatcgc gccgacgccg agttgcggct ttgaggagtg cgcgtccttg ccggtgattt
     4561 cggtgcggta gcgatagccg cccttgtggg catcgacgac gctcatcaag gacggctcgc
     4621 cgacgaacac cacgccgggc ttcggcagcg acttgccgaa ctcggcgatc atcggcttca
     4681 cgcccgtgca gccgacctcc tcgtcgtagg agaacgcgat atgcagcggc gtcttcaatc
     4741 gtcgtcgctt gtagagcggt acggtcgcga gcacgcaggc gaggaacccc ttcatgtcgc
     4801 aggtgccgcg gccatagagc cggccatcct tctcgaccat tgtgtaagga tccgtctccc
     4861 acggctgacc ggtgaccggc accacatcca tgtggcccga gagcgcgatg ccgccgttgc
     4921 cggctggccc aaccgtcgcg aacaggtttg cccagttgcc gtcgggtgat ggcagtagcc
     4981 gcgcctcgac gccctcgctc gcaagaaaat cgcggatcca ttccgcgcac ggcacgttgc
     5041 tcttgtggct cgtcgtgtcg aacgagacga gcctccccag gatctccttc gcggtgtgga
     5101 tgctggcggc catgagcgtc tgtccccggt ctgtttcgag cgagcattgg ccgggccggc
     5161 atggccggtc aaggcagatg cgccagccgc ccttgcggga cagcccccgg caagacgcat
     5221 gttgcgggcg gccctcgcca catgccagtt attgccgaac gatnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnncccgcc gcatgaccgg cggtcaagca ctcgccgaga
     5341 tgctgaagct cgcggacgtc gggccgatgt tcggcatggg tggcttccag ctgctcccct
     5401 tctacgaggc ctgccgggcg ctgaagttgc agcactatct catcaacgac gagcgctcgg
     5461 gcgccttcgc ggccgatgcc tacgccaagg tgacgaaccg gccgggcgtg gtcgatgcga
     5521 cgctcgggcc gggcgccacc aacctgttga caggcctcgt cgagagcctc aacgccggcc
     5581 tccccatgat cgccatcgtc ggcgatgcca accgcgagca tgcctggaag aacatgacgc
     5641 aggagacgcg gcagatcgag atgctgcgcc ccgccgtcaa ggaggtgatc cgcgtcgagg
     5701 tgatcaagcg gctgccggag cacgtccggc gcgccttcgc cgtcgcgacg agcggccggc
     5761 ctggtccggt catcatcgac gtgcccgagg acatcgccca cggcgagcac gacttcgacg
     5821 cggccgattt ctggatcgat cccggcactc tgaaagctca agcccgacgc ctccgcgccg
     5881 atccggccga acttgcgcgc gcagtcgctc tcctcgccaa ggccgagcgg ccgatcatca
     5941 tggcaggcgg cggcgtgcac atctccgatg cccacgccgc actgcagacg ctcgccgagc
     6001 gccacggcat ccccgtcgcc cacacgatga cggggaaggg cgcgatcgcc tgcacccatg
     6061 cgctgtcggc gggcctcttc ggccgctatg accgcatcgc caacggcatg atcgcggaat
     6121 cggattgcct catcgtcgtc ggctgcaagc tcggcgagat cgcaacgaag cgcttccagc
     6181 tgatcccgcc ggggaaaccc gtcatccacg tcgagattgt ccccgacgag atcggccgca
     6241 ccacgaggac cgacatcggc ctcgtaggtg acgcccgcct cgtcatcgag gacatcacgg
     6301 cggcactcgg tgacggcaag gcggccgccg ggaggcgagc acaatggtgc gccgaggtgc
     6361 cgaagcgcat ggccgcctgg cgcaagagcg cggcggagcg tgtggaatcg aaggagagcc
     6421 cgatcaacgt cggccgcctg atgggcgagc tcaacaagat catgccggcg gacggcatcg
     6481 tcgtcgccga cggcggcttt gctgcgcact ggggtggcct cttcttcgat acgaagaagg
     6541 ccggccgcca tttcctgccc gatcgtgggc tcgcatcgat cggctacggc gtcccgggcg
     6601 gtatcggcgc gcagatcgca gcgggcaagc gccgcgtcgt ctcactcacc ggtgacggcg
     6661 gcttcaacat gagcctcggc gagctcgaga cggctcgccg cgtcggcgcg aacttcgtcg
     6721 ccatcgtctt caacaacgcg gctt
//
LOCUS       DAORNH010000254         6730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8410_length_6730_cov_2.603745, whole genome shotgun sequence.
ACCESSION   DAORNH010000254 DAORNH010000000
VERSION     DAORNH010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8410_length_6730_cov_2.603745"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>748)
                     /gene="glyS"
                     /locus_tag="PK264_19920"
     CDS             complement(<1..>748)
                     /gene="glyS"
                     /locus_tag="PK264_19920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRD78173.1"
                     /translation="KSMRWGMSSETWVRPLHSIICLFDGKVVPFTWGGVASGRATRGH
                     RFHGNDPFEVKGFEDYATKLKERKVLLLASERALMIREQAQEIAAKAKLEIVEDEGLL
                     AENAGLVEWPTVMLGTFEREFLEVPAECLTTAMRTHQKCFSLKDPKTGTLANRFLLVS
                     NLIATDGGKTIVAGNEKVIRARLSDARFFWTQDLKRPLDEMASDLAGITFHEKLGSQK
                     DRVERISELAHEIAGAVDADPDSARRAAQLA"
     assembly_gap    749..758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<759..1221)
                     /locus_tag="PK264_19925"
     CDS             complement(<759..1221)
                     /locus_tag="PK264_19925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRD78174.1"
                     /translation="MSELLIELFSEEIPARMQARAASDLAQAVLAALETEGLGAGLAK
                     DGGQKGQSTAAEARLKTVSCSTPRRLVLVVDGLPAQSPTVVDERKGPRVGAPDAAIQG
                     FLKGAGIASIGDARVVEDKKGSYYVATIEKKGRPTAEIIAAIMPAVLSKFSW"
     assembly_gap    1272..1289
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1340..1738)
                     /locus_tag="PK264_19930"
     CDS             complement(1340..1738)
                     /locus_tag="PK264_19930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD78175.1"
                     /translation="MAWKRLIPMLSVRDLKRTIAFYREELGFELVASFGDPEPVWCHL
                     KRDQTHFMFNAPGESDLEAIPARARDFQIFYIYPDDVTVLHGDLAAKGLSVTPLRITL
                     YGMREFELRDPDGHWLWFGETTTEPPTLSE"
     gene            complement(1829..2929)
                     /locus_tag="PK264_19935"
     CDS             complement(1829..2929)
                     /locus_tag="PK264_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78176.1"
                     /translation="MRSQLFRWSTLAAGLVVLLPVTAGAVGKAPEIRTHAGNPVPECV
                     SPRRLMLFLKERNPGLDPVYEDIAKLYKAHGDANRVRWDYAFYQMLIETNYLSYRRGS
                     GWGDAKPKQFNFAGLGTTGRGVPGEAFPDVSTGVLAQIQHLVAYAGDKVENPVAKRTR
                     DNQDGIIAISRRLGRTVTWADLTRRWAMDRRYWTMIESIADRFRTAHCHGVDEQDETR
                     VVAKAPPRPEPARRKAAKSAAPVTPAPTAAPSPQPASILPNPAAHARSSLGGPLPGPA
                     PTPAASPSAACRIFTASYGGTRNALISVSIGGETHLTALQVLDGQEQALADAFIADHA
                     RGGELLGVFESRSDAISRARVICPAAVPPTRG"
     gene            complement(3131..4387)
                     /locus_tag="PK264_19940"
     CDS             complement(3131..4387)
                     /locus_tag="PK264_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRD78177.1"
                     /translation="MKYRVGALALLSVPLAISAGAASQSMPPVKVSAHNQVPACVTPG
                     RLMSFIKSRNDKLDPRFAKIAVDYMRHGETLGIRWDFAFVQMAIETGYLSFTREGSRR
                     GDVAARQNNFAGLGATGNGEAGESFGDVSTGVLAHLQHVLIYAGERVDNAVAERTRKV
                     QQWGVLKSWQKHVKGPFTWAHVAKRWATDEAYAEAIDSHAKRFFAEFCNKSDPEPGLV
                     AEARKGRAPAATAAVPADAPTTPGADLARKALADAKGDRTVRRSGLGAGLIAKSVDPT
                     AVSAPDAGDAAATIAPSAATAATAGGAPKVKPKAAPAAPVAQPVAIPKLTAPPAAPAV
                     ASPAVPPGSECRVWTASYGGSRAIIIRSMTGKTINLTVLDVNEGQERREADAYISAYA
                     KGGMLLEEFASQAVALDKAFELCPEG"
     gene            complement(4697..6106)
                     /locus_tag="PK264_19945"
     CDS             complement(4697..6106)
                     /locus_tag="PK264_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRD78178.1"
                     /translation="MMRRKPEFAFWTFLALAVLAGATTVLNVSRTYSASGNSEIFKKL
                     DLFGDVVERVRNDYVEKPDDSMLIDAAINGMLNALDPHSAYLNPKSFRDMQISTRGEF
                     GGLGIEVTMENNVVKVVSPIDDTPAHKAGVLANDFITHLDGESIVGLALDQAVEKMRG
                     PVNTPIVLTIRRQGREEPLEIRIVRDIIKIQAVKWKTIGDIGYVKISTFNEQTHVNLV
                     KAVDEIKKKLGTRVKGYIIDLRNNPGRLLEQAIAVTDDLLDEGAIVLTKGRNLEETQR
                     ANARRGDITEGKKVVVLINGGSASASEIVAGALQDHKRAIIIGTRSFGKGSVQTIIPL
                     GQNNGAIRLTTARYYTPSGRSIQAKGIDPDMLIEQDLPPELKAKTPERPRGEASLRGH
                     LKGDGSKAGERETSGSSAYVPREEEKDTQLQFALKLLLGEVTGPPTPADMKGPAPTAP
                     APAPQPGVVPPAPPAGDKK"
     gene            complement(6103..6666)
                     /locus_tag="PK264_19950"
     CDS             complement(6103..6666)
                     /locus_tag="PK264_19950"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRD78179.1"
                     /translation="MAADGERPKLAAVQPIPKSALPAPAVEIAPKGTFTSFSPGRIAP
                     AMPFAKALKQLRLPVHGQRVSSFGDKSARGNSQGIYISTRHDARVTAPADGWVVFAGL
                     FKQLGPTLIISGGDGYHIVLLGMSQIDVQVGQFVLMGEPVGTMPAAPKTAAKSQGTNP
                     VLYIEFRKDGRPINPDPWWADPATKVQ"
BASE COUNT         1076 a         2169 c         2221 g         1236 t
ORIGIN      
        1 ggcgagctgg gccgcccggc gcgcactatc cggatcggca tcgacagcgc ccgcgatctc
       61 gtgcgccagc tccgagatcc gctcgacgcg gtccttctgc gagccgagct tctcgtggaa
      121 tgtgatgccg gcaagatcgg aggccatctc gtcgagtggc cgcttcaagt cttgcgtcca
      181 aaagaagcgg gcgtcggaga ggcgcgcgcg gatcaccttc tcgttgccgg cgacgatggt
      241 cttgccgcca tccgtggcaa tgaggttcga gacgagcagg aagcggttgg cgagcgtccc
      301 cgtcttcggg tccttcagcg agaaacactt ctggtgcgtg cgcattgccg tcgtcaggca
      361 ctcggccgga acctcgagga actcacgctc gaacgtcccc aacatcacgg tcggccactc
      421 gacgaggccg gcgttctcgg ccagcagccc ctcgtcctcg acgatctcga gcttggcctt
      481 cgccgcgatc tcctgcgctt gctcgcggat catcagggcg cgctcggagg cgagaaggag
      541 gactttgcgc tccttgagct tcgtcgcgta gtcctcgaaa cccttcacct cgaacggatc
      601 gttgccatgg aagcggtggc cgcgcgttgc tctaccgctc gcgacgccac cccacgtgaa
      661 tggcacgacc ttgccgtcga acaggcagat gatcgagtgc agcgggcgca cccaggtctc
      721 gctgctcatc ccccagcgca tcgacttcnn nnnnnnnngc caggagaact tcgagagtac
      781 cgccggcatg atcgccgcaa tgatctcggc cgtcgggcga cctttcttct cgatggtggc
      841 gacgtagtag ctgcccttct tgtcctcgac gacccgtgcg tcccctatgc ttgcgatgcc
      901 cgcgcccttg aggaagccct ggatcgcggc atcgggcgct ccgacccgcg gtcccttgcg
      961 ctcatcgacg acagtcgggg actgcgcggg cagcccgtcg acgaccagca cgaggcggcg
     1021 aggtgtcgag caggagacgg tcttcagacg tgcctcggcg gcggtcgact gccccttctg
     1081 tccgccgtcc ttggccagac ccgcgcccag cccctcggtc tccagcgccg cgagcaccgc
     1141 ttgcgcgagg tcggaggccg cgcgtgcctg catgcgcgcg gggatctcct cggagaagag
     1201 ctcgatcagc agctcggaca tggcattggc tcgaacgaaa gggcggctgc acgacggggc
     1261 agccttggac cnnnnnnnnn nnnnnnnnnc gggtcaatca attgcgacga gcggtccggg
     1321 ccaccttctg ccagcgctat cactccgaga gggtcggcgg ttcggtggtg gtctcgccga
     1381 accagagcca atgcccatcc gggtcgcgca gctcgaactc gcgcatgccg tagagggtga
     1441 tgcgcagcgg cgtgacggaa aggccctttg ccgccagatc accgtgcagc accgtcacgt
     1501 catcgggata gatgtagaaa atctggaaat cgcgggcgcg tgccgggatg gcctccagat
     1561 cagactcgcc cggcgcattg aacatgaagt gcgtctgatc gcgcttcaga tggcaccaga
     1621 cgggctcggg gtcgccgaag gatgccacca gctcgaagcc gagctcctcc cgatagaagg
     1681 caatggtgcg cttgaggtcg cgcacgctca acatcggaat gagccgcttc caggccattt
     1741 ctcccccgtg gtgcccctcg atgcgatgat cagatagaac ccggacgggg cgcggcaagc
     1801 gtgttcatcc gccgcgggat gatttccgtc agccgcgcgt cggtggcact gctgcggggc
     1861 agatcacgcg cgctcgcgat atggcgtccg aacggctctc gaacacgccg agtagctcgc
     1921 ctccgcgtgc gtgatcggca atgaatgcgt cggccagcgc ctgctcctgg ccgtccagca
     1981 cctggagcgc cgtgagatgg gtctcgccgc cgatgctgac cgagatcagc gcgttgcgcg
     2041 tgccgccgta gctcgcggtg aagatgcggc aggcggcgct cggtgacgca gcgggcgtcg
     2101 gggcggggcc cggcagcggg ccgccaaggc tcgagcgggc atgagctgcg ggattcggca
     2161 ggatggacgc cggctgaggg ctcggggccg cagttggggc cggagtgacg ggcgccgcag
     2221 atttcgcagc cttgcggcgc gctggctccg gccgaggcgg cgccttggcg acgacccgcg
     2281 tttcgtcctg ctcgtcgacg ccgtggcaat gagccgtgcg gaagcggtcg gcgatggatt
     2341 cgatcatcgt ccagtagcgc cggtccatgg cccagcggcg cgtgagatcc gcccaggtca
     2401 ccgtgcggcc gaggcgacga gagatggcga tgatgccgtc ctggttgtcg cgcgtacgct
     2461 tggcgaccgg gttttcgacc ttgtcgccgg cgtaggccac gaggtgctgg atctgggcga
     2521 ggacgcccgt cgacacgtcg ggaaaggcct cgcctggaac gccgcgtccc gtcgtgccga
     2581 gaccggcgaa attgaactgc ttcggcttgg catcgcccca gccgctgccg cgtcggtagg
     2641 agagataatt cgtctcgatc agcatctgat agaaggcata gtcccagcgg acgcggttcg
     2701 catcgccatg cgccttgtag agcttggcga tgtcttcata gaccggatcg agacccggat
     2761 tgcgctcttt gaggaaaagc atcaggcggc gcggcgacac gcactcggga accggattac
     2821 cggcatgtgt cctgatctcg ggcgccttgc cgacggcgcc cgccgtcacg ggcagcagca
     2881 ccaccaaacc agccgcgagc gtcgaccagc gaaagagctg cgatcgcaag agctgtctcg
     2941 cggaggcggc attgcctccg ttcccatcaa gtccgaccat gaccgtcccc gtctgttttg
     3001 cagagcatca gcgcgctttg cgggtcttga ttgcccgctt gcgcgctttt ctgatttcgg
     3061 cgtgtgccgc cgcagtcttc ttggaaatcg tccgaacgtg catcgcaccc ttcccgtggc
     3121 gcgatcctat tcagccctcg gggcagagct cgaaggcctt gtcgagggca acagcctggc
     3181 tcgcgaattc ctcgagcagc atgccgccct tggcgtaggc cgagatgtag gcgtcggcct
     3241 cgcggcgctc ctggccctcg ttgacgtcga gcaccgtcag attgatggtc ttgcctgtca
     3301 tcgatcggat gatgatggcg cgcgatccgc cgtagctcgc cgtccacacg cggcactcgc
     3361 tacctggcgg tactgcgggt gaggcgaccg ccggcgcagc aggtggcgct gtgagtttcg
     3421 ggatcgcaac cggctgggcg acgggcgcag caggagccgc ttttggcttc actttcggcg
     3481 cgccgccggc tgtcgccgcg gttgctgccg aaggagcgat ggttgccgcg gcatcgccgg
     3541 cgtcaggcgc ggacacggcc gtcgggtcga ccgacttggc aatcaagcca gcacccaatc
     3601 cggagcggcg aaccgtgcga tcgcccttag cgtcggcaag cgccttgcgg gcgagatcag
     3661 cgcccggggt cgtcggcgca tcggcaggca ccgcagcagt tgccgcgggc gcgcgaccct
     3721 tgcgcgcctc ggcaacgagg cctggttccg ggtcggactt gttgcagaac tcggcgaaga
     3781 agcgcttggc gtggctgtcg atcgcctcgg cataggcctc atcggttgcc cagcgcttgg
     3841 cgacatgcgc ccaggtgaac gggcccttga cgtgcttctg ccacgacttg agtacgcccc
     3901 actgctgcac cttgcgcgtg cgctcggcga cagcgttgtc gacgcgctcg cccgcgtaga
     3961 tgagcacgtg ctggaggtgg gcgagcacgc ccgtcgagac gtcaccgaag ctctcgccgg
     4021 cctcgccatt cccggtcgcg ccgaggccag caaaattgtt ctggcgggcc gcaacgtcac
     4081 cgcgacggct gccttcgcgc gtgaagctca ggtagcccgt ctcgatggcc atctgcacaa
     4141 aggcgaaatc ccagcggatg ccgagcgttt cgccatgccg catgtaatcg acggcgatct
     4201 tcgcaaaacg cggatcgagc ttgtcgttgc gggacttgat gaaggacatg aggcgcccgg
     4261 gcgtcacgca cgcgggaacc tgattatgcg ccgatacctt cactggcggc atcgactggg
     4321 acgcggcccc tgccgaaatc gcgagcggca cggacagcag tgcgagagca cctacgcggt
     4381 acttcatgga ctgacaaccc tggctacctg ctacaactct gttttgcgcc tgtttttagt
     4441 taaattggtt atatgcgctt cgagttgagg ttccattacc gcccccgttc ggcggtctta
     4501 cacactttct caacttggtt tgaggcgcga gtgcgaagct cgaagggagg aattgtggca
     4561 atcccgcgat ttcctcgggc ttgccacgga aaaagtgctt cagacgcgcg aaaggcgccc
     4621 cgctctcgcg cgggacgccc tcctcgtctc gttcgattgg ccggacagat tgtccgccga
     4681 ccgctctgaa ctcagactac ttcttgtcgc cggcgggcgg cgcgggcggg acaacacccg
     4741 gctgcggcgc cggagccggc gcggtcggtg ccggaccctt catgtcagca ggcgtcggcg
     4801 ggccggtcac ttcgccgagc agcagcttca gcgcgaactg gagctgtgtg tccttctcct
     4861 cctcgcgggg gacataggcg gacgaacccg acgtctctct ctcccctgcc ttcgagccgt
     4921 cacccttgag atggccgcgc aaggatgcct cgccccgtgg ccgctccggc gtcttggcct
     4981 tgagctctgg cggcagatcc tgctcgatca gcatgtcggg atcgatgccc ttggcctgga
     5041 tcgagcggcc cgacggcgtg tagtagcggg ctgtcgtaag gcggatcgca ccgttgttct
     5101 gcccgagcgg gatgatggtc tgcaccgagc ccttgccgaa cgatcgcgtg ccgatgatga
     5161 tcgcgcgctt gtgatcctgg agcgcaccag cgacgatctc ggatgcagag gccgagccgc
     5221 cgttgatgag aaccacgacc ttcttgcctt cggtgatgtc gccacgacgc gcgttcgccc
     5281 gctgcgtttc ctcgaggttg cgccccttcg tcagaacgat ggcgccttcg tcgagcagat
     5341 cgtccgtgac ggcaatcgcc tgctcgagca ggcggcccgg gttgttgcgc aggtcgatga
     5401 tgtagccctt cacgcgcgtg ccgagcttct tcttaatctc atcgaccgcc ttcacgaggt
     5461 tgacgtgggt ctgctcgttg aaggtcgaga tcttgacgta gccgatatcg ccgatcgtct
     5521 tccatttcac cgcttggatc ttgatgatgt cgcggacgat acggatctcg agcggctcct
     5581 cgcggccctg gcggcggatg gtgagcacga tcggcgtatt gaccgggccg cgcatcttct
     5641 cgaccgcctg gtcgagcgcc agaccgacga tcgactcgcc gtcgaggtgc gtgatgaaat
     5701 cgttggcgag gacacccgcc ttgtgggccg gcgtgtcatc gatcggcgag acgaccttga
     5761 cgacgttgtt ctccatcgtc acctcgatgc cgaggccgcc gaactcgccc cgcgtcgaga
     5821 tctgcatgtc gcggaagctc ttcgggttga ggtaggccga atgcggatcg agtgcgttca
     5881 gcatgccgtt gatggcggca tcgatcagca tcgagtcgtc cggcttctcg acgtagtcgt
     5941 tgcgcacccg ctcgaccaca tccccgaaca gatcgagctt cttgaagatc tcggaatttc
     6001 ccgaggccga gtaggtcctg gacacgttca ggaccgtcgt cgcccccgca agaacggcga
     6061 gcgccaggaa tgtccagaac gcgaattcgg gtttgcgcct catcattgca cctttgtagc
     6121 cggatccgcc caccatggat cgggattaat cggccggccg tccttccgga actcgatata
     6181 taggacggga ttcgttccct gcgacttcgc cgccgtcttc ggagcagcag gcattgtgcc
     6241 aaccggctca cccatcagca cgaactgccc gacttgaacg tcgatctgcg acatgccgag
     6301 cagtacgata tgatagccgt ccccaccgct gatgatcaag gtcgggccga gctgcttgaa
     6361 gaggcctgcg aataccaccc agccgtcagc gggagctgtc acgcgtgcat cgtgccgcgt
     6421 cgagatgtag atgccctgcg agttgccgcg ggcggacttg tcgccgaagc tcgaaacgcg
     6481 ttgaccgtga acgggcaggc gcagttgctt cagagccttg gcgaaaggca tggccggcgc
     6541 gatgcgcccg gggctgaagc tcgtaaatgt gcccttgggg gcgatctcga cggccggcgc
     6601 cgggagagcc gactttggta tgggctgcac cgcagcgagt ttcggccgtt caccatcagc
     6661 ggccactggg gcggcaccgg acggcgcaag tggcggcggc tatgagtccg agggcggttc
     6721 gctcggcgcc
//
LOCUS       DAORNH010000255         6726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8419_length_6726_cov_2.587018, whole genome shotgun sequence.
ACCESSION   DAORNH010000255 DAORNH010000000
VERSION     DAORNH010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8419_length_6726_cov_2.587018"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    246..388
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<389..673)
                     /locus_tag="PK264_19955"
     CDS             complement(<389..673)
                     /locus_tag="PK264_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRD78180.1"
                     /translation="MATNPHITLLKAPTLADFEALAADAWAVIPEEFRKRCNNLVIQV
                     EDFPTDEVLDALGIESPFDLMGLYQGVSLEKKSVMDAAPEQDLVYLYRRPM"
     gene            complement(686..2668)
                     /gene="thrS"
                     /locus_tag="PK264_19960"
     CDS             complement(686..2668)
                     /gene="thrS"
                     /locus_tag="PK264_19960"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRD78181.1"
                     /translation="MTDISLTFPDGAKRQVKRGTTGLEVAKSISPSLAKRTVAMSLDG
                     VVADLADGIESDAAIKFLSRTDPEALELIRHDCAHVLAEAVQELWPGTQVTIGPVIEN
                     GFFYDFAKAEPFVPEDIPKIEKRMGEIIARNVPFTKEVWSRDKAKAHFKAKGEHFKVE
                     LVDAIPAGEELKIYSQGSWLDLCRGPHMTATGHIGKAFKLTKFAGAYWRGDAKNAQLQ
                     RVYGVAFAKDEELAAYLKQQEEAEKRDHRKIGREMDLFHFQEEGPGVVFWHAKGWTTF
                     QSLIAYMRRRLADDYQEVQGPQLLDKSLWETSGHWGWYSDNMFAVKSATAFMNPKDES
                     ADQKVFALKPMNCPGHVQIFKQGSKSYRDLPTRLAEFGCVHRYEASGALHGLMRVRGF
                     TQDDAHVFCTEEQLEAECLKINDLMMSVYKDMGFEEVMVKLATRPAKRVGTDAMWDHA
                     ESILRKVLARMAASDNRIKTGINEGEGTFYGPKFEYTLKDAIGREWQCGTTQVDFNLP
                     ERFGATYVAADGEKRRPVMVHRAICGSLERFLGILIESTAGHLPLWLAPVQATVCTIV
                     SDADGYAAEVVTALKRAGLRADVDLRNEKINYKVREHSVAKTPILLVVGKREAEERTV
                     SIRRLGSQASETMSLDAVIAALTTEALPPDVVRARA"
     gene            3323..4825
                     /locus_tag="PK264_19965"
     CDS             3323..4825
                     /locus_tag="PK264_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRD78182.1"
                     /translation="MAKRVLIVDDDPVQRRLLEECVKRSGYEVKCAAGGEQALQILEG
                     PDRGGIALVLLDLVMPGADGLAVLDRLNARPGVPPIIVQTANGSLDAAINAMRLGASD
                     FVVKPVSPERLEVSMKSALKIEALQGEITRIKKKAEGTLTFSDLIIRGEAMLRVITQG
                     KKVASSNIPVLIEGESGVGKELIARAIQGESERRSKPFVTVNCGAIPENLVESTLFGH
                     EKGSFTGAVDRRVGKFQEADGGTLFLDEIGELPLDAQVKLLRALQQGEIDPVGSKRPI
                     KVNFRLVSATNRDMIQLVKDGKFREDLYYRISGFPILMPPLRDRIEDVPELARHFLAR
                     FAAEEGKRIAAIAPTALELLMSYSWPGNVRQLENAIFRAVVLAEGSELTIAEFPQIAA
                     HVEGFAAEIPAAPAQVDDTPTFTGPALLGAEDLVPRTVELGGGKGTLGIPAVDDEGEI
                     RSLEAIEADMIRLALGRYRGHMTEVAKRLNIGRSTLYRKMQYYGLEPRVG"
     gene            5277..>6726
                     /locus_tag="PK264_19970"
     CDS             5277..>6726
                     /locus_tag="PK264_19970"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRD78183.1"
                     /translation="MLRRLALSVVLAGGFMSGSALGIEPDAPERLLTTEDLVKSVLRD
                     VVARMKPTASPRDRQERAAISAFYEGRDWRPAWVDAKGLNDKGRAIVEEIKAADDWGL
                     VASTFELPNLAGAGAALGNQVIAAEDLRLTVAILKYAREARGGRTDPSVQLSKNLDRT
                     PALFEPRSVLDETATSEDPSAYLRRLHPQHPQFERLRKAYVALKAKGHVAIEKIPDGP
                     RIAPGTSHPHVAIVRRRLGLASLGDDGRDMFLDEAVARALREFQSARGITPASSAIDL
                     ATRRAFNAVEGGSPKRLLANMEQWRWMPDDLGAFHVWVSIPDFTVRVMKDGQVIWSER
                     IIVGKVDTQTPIFSDEMEFVVFHPEWGVPDSIKVKELWPSLRHSTSILAKQNLRVMHN
                     GRVIDPDSVDWAGVDPRTFSFIQTSGNGNVLGVVKFRFPNKHDVYMHDTPTKGLFSSA
                     VRAYSHGCMRVQNPVRLAEIVLAHDKQMAAERV"
BASE COUNT         1089 a         2154 c         2162 g         1178 t
ORIGIN      
        1 ccacgcaaaa caccagccga gtaagcccgg tcctagagtt ccgcgtgccc gtgtcgatgc
       61 aaccaccctc acccgtcctg cagcaaagat caacggcatg cggttcggca agtgaacatt
      121 tcgccgatcg ctgcggcgga cccgtcacat ctcgccagcg atcgtggcag tcctgcggcg
      181 gtcatgttgc aatccacgct cgggagcccg cgggctcacg gcgcccgcca atcgtcgtga
      241 gcggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tcggccggcg atagaggtag acgaggtcct
      421 gttcgggcgc ggcgtccatc acgctcttct tctcgaggct cacgccttgg tagaggccca
      481 tcaaatcgaa agggctctcg atgccgagcg cgtccagcac ctcgtcggtc gggaaatcct
      541 cgacctggat gacgaggttg ttgcagcgct tgcggaactc ctccgggatc accgcccaag
      601 catcggccgc cagcgcctcg aaatcagcga gcgtgggagc cttgagcaag gtgatgtgag
      661 ggttggttgc catcggcctg ctccatcacg cccgcgcccg caccacgtcc ggtggcaggg
      721 cctcggtggt cagcgccgcg atcacggcat cgagtgacat ggtctcgctc gcctggctgc
      781 cgaggcggcg gatggagacc gtgcgctcct cggcctcgcg cttgccgacg acgagcagga
      841 tcggcgtctt cgcgacggag tgctcacgca ccttgtagtt gatcttctcg ttgcgcaagt
      901 cgacgtccgc gcggaggccc gcgcgcttca aggccgtcac gacctcggcg gcgtagccgt
      961 ctgcatccga gacgatcgta cacaccgtcg cctgtacggg cgcgagccag agcggcagat
     1021 ggccggccgt gctctcgatg aggatgccga ggaagcgctc gagcgagccg cagatcgcgc
     1081 gatgcaccat aaccgggcgg cgcttctcgc cgtcggcggc gacataggtt gcgccgaaac
     1141 gctcaggcag gttgaagtcg acctgcgtcg tcccgcactg ccactcgcgg ccgatcgcgt
     1201 ccttcagcgt gtactcgaac ttcgggccgt agaacgtgcc ttcgccctcg ttgatgccgg
     1261 tcttgatgcg attgtcggaa gctgccatgc gggcgaggac cttgcgcagg atgctctcgg
     1321 cgtgatccca catggcgtca gtgccgacgc gctttgccgg ccgggtcgcg agcttcacca
     1381 tcacctcctc gaagcccatg tccttgtaga cggacatcat gaggtcgttg atcttcaggc
     1441 actcggcctc gagctgctcc tcggtgcaga atacgtgggc atcgtcctgc gtgaaaccgc
     1501 gcacgcgcat gaggccgtgc agcgcgcccg aggcctcgta gcggtgcacg cagccgaact
     1561 cggcgagcct cgtcggcagg tctcggtagc tcttcgagcc ctgcttgaag atctgcacgt
     1621 gacccgggca gttcatcggc ttcagcgcga acaccttctg gtctgcgctc tcgtccttcg
     1681 ggttcatgaa ggcggtcgcc gacttcaccg cgaacatgtt gtcggagtac cagccccagt
     1741 ggcccgacgt ctcccagagc gacttatcga ggagctgcgg gccctgcacc tcctggtagt
     1801 cgtcggcgag gcggcggcgc atgtaggcga tgaggctctg gaacgtcgtc cagcccttcg
     1861 catgccagaa gacgacgccc ggcccctcct cttggaagtg gaagaggtcc atctcgcggc
     1921 cgatcttccg gtgatcgcgc ttctcggctt cctcctgctg cttcaggtag gcggcgagct
     1981 cctcgtcctt ggcaaaggca acgccgtaga cgcgctgcaa ctgcgcgttc ttggcatcgc
     2041 cccgccagta ggcgcccgcg aactttgtga gcttgaacgc cttgccgatg tggcccgtcg
     2101 ccgtcatatg cgggccgcgg cagaggtcga gccagctgcc ctgcgaatag attttcaact
     2161 cctcgccggc cggaatggcg tcgacgagtt cgaccttgaa atgctcgccc ttcgccttga
     2221 agtgcgcctt cgccttgtcg cggctccaca cctccttcgt gaacgggacg ttgcgggcga
     2281 tgatctcgcc catgcgcttc tcgatcttgg ggatgtcctc cggcacgaac ggctcggcct
     2341 tggcgaagtc gtagaagaag ccgttttcga tgacagggcc gatcgtcacc tgcgtgccgg
     2401 gccaaagctc ctgcacggcc tcggcgagga cgtgagcgca gtcgtgccgg atgagctcca
     2461 gcgcttctgg atcggtgcgc gagaggaact tgatcgccgc gtcgctctcg atgccatcgg
     2521 cgaggtcggc gacgacgccg tcgaggctca tggcgaccgt gcgcttggcg agcgaaggcg
     2581 agatcgactt ggcgacctcg aggccggtcg tgccgcgctt cacctgacgc ttggcgccgt
     2641 cggggaaggt cagcgaaatg tcggtcatgg ccggtttctc ctcggttcgt ccggccgcgc
     2701 gagcggcgga cgagccgaga taatcctttg ctcaatcggg agcaagacgg gaggggctcc
     2761 ggcatggcgc atgcgcgacc ggatcgcctg ccggcgcgct cgcgtgaggt cgagtgccag
     2821 ctatcggcgg cagatgagct tccggcacgg gttcggcggc agagggcgcg ggcgaatgat
     2881 gacacggtgc gccgagccgg tgcggacaac tgcgggcccg ggcgggccag cagttgtgcc
     2941 tgccggcggc ggcaagggca cggccgacgc cacgctcgct gcctgcagaa cgcccgccgc
     3001 cagcagcggg caaaggatga aacaacgcaa tctcggcatg atgctctccg gtcagttgga
     3061 gggttgtggt cgaggtcctc aaatcatgag tgcgcctacc ggagcgaaac cttgggcgtc
     3121 gatacgagat tgaaagcttg ttgccgcggg cctcgaaatg aaagcctcag ggcggcgcgc
     3181 ttctcaatat cttgtttacc ggtctcaggc actctctcgt ctgtgagcga ggtgccgagt
     3241 tgcggcgcgc cctgtcgggg cgccgccatc gtcgaggctc ccgcatcgtg tgtgtcagtc
     3301 gagtagtcga gtagctgtac ccatggcgaa gcgcgtcctg atcgtcgatg acgatcccgt
     3361 ccagcggcgt ctgctggagg agtgcgtcaa gcgctccggt tatgaggtga agtgtgcggc
     3421 cgggggcgag caggcgctcc agatcctcga aggtccagac cgcggcggca tcgcgctcgt
     3481 cctcctcgac ctcgtgatgc ccggcgccga cggcctcgcc gtgctcgacc gtctcaacgc
     3541 caggccgggc gttcccccaa tcatcgtgca gacggccaac ggctcgctcg atgccgcgat
     3601 caacgccatg cgcctcggcg cctccgattt cgtcgtgaag ccggtgagcc ccgagcggct
     3661 cgaggtttcc atgaagagcg cgctcaagat cgaggcgctg cagggcgaga tcacgcgaat
     3721 caaaaagaag gcggaaggga cgctcacatt ctccgacctc atcatacgcg gcgaggccat
     3781 gctgcgggtg atcacgcagg gcaagaaggt cgcgtcctcc aatattcccg ttctgatcga
     3841 gggcgagtcg ggcgtcggca aggagctgat cgcacgcgcc atccagggcg agagcgagcg
     3901 gcgctcgaag ccattcgtga cggtcaactg cggcgcgatc ccagagaacc tcgtcgagag
     3961 cacgctgttc ggccacgaga agggctcgtt cacgggcgca gtcgacaggc gggtcggcaa
     4021 gttccaggag gccgacggcg gcactctgtt cctcgacgag atcggcgagc tgccgctcga
     4081 cgcccaggta aagctcctgc gcgcgctgca gcagggcgag atcgatccgg tcggctcaaa
     4141 gcgcccgatc aaggtcaatt tccgcctcgt ctcggcgacc aaccgggaca tgatccaact
     4201 cgtcaaggac ggcaagttcc gcgaggacct ctactatcga atctccggtt tcccgatcct
     4261 gatgccgcca ctgcgcgacc gcatcgagga cgtgcccgag ctcgcccgcc acttcctggc
     4321 ccgcttcgcg gccgaggagg ggaagcgcat cgcagccatc gcaccgacgg ccctcgagct
     4381 gctgatgagc tattcctggc ccggcaatgt gcgccagctc gagaatgcga tcttccgcgc
     4441 cgtggtgctc gcggaaggca gcgagctcac gatcgccgag ttcccgcaga tcgcggccca
     4501 cgtcgaaggg ttcgcggccg agattccggc ggctcctgca caggtcgacg atacgccgac
     4561 cttcaccgga ccggcgctgc tcggtgccga ggacctcgtt ccgcgcacgg tcgagctcgg
     4621 tggcggcaag ggcacgcttg gcattccggc cgtagacgat gaaggagaga tccgctctct
     4681 cgaggcgatc gaggccgaca tgatccggct ggcgctcgga cgctatcgcg gtcatatgac
     4741 cgaggtggcc aagcgtttga acatcggccg ttccacactc taccggaaga tgcagtacta
     4801 tgggctcgag ccgcgcgtcg gctgatctcg caccttcgag tttcgtgatg ccggcctcgc
     4861 caaaagcggc tgccatgcgt ccaacgaggc gtggcagcgc cgtgcggctt tcgcgtcgat
     4921 cgtaattgcc gattgcggcc ccgacagttc agcgcggcgc gcgcacgatt tcccgtgcgc
     4981 cgtatcatag cgtgcgcacg cgacgaactc gcgccacaat tgtcacggcg tcatctcttg
     5041 accgctcagc acgcagcgtc gtttggcgac gctgcgttgc acgtatgctc gatccgagca
     5101 ttcccgcgat ccaggttgcg ggcgagcgcg atgatggtct acgagccgca ctctatcggt
     5161 gcggctgttg ggcaacagct ggcgcagctc acgacagtgt gctggatcgt gtttggcaat
     5221 cggtggcgtc tgcggctagc gtgcgcgccg agagagtatt gtggtcggag cggagcatgc
     5281 tgcgtcgtct cgcattgtcg gtcgtcctcg ccggcggttt catgtccggc tcagctttgg
     5341 gcatcgagcc tgatgcgccc gaacggctcc tcaccaccga ggatctcgtg aaatcggtgc
     5401 tgcgcgacgt ggtggcgcgc atgaagccga cggcgagccc gcgggaccgg caggagcgcg
     5461 ccgcgatctc ggccttctat gaaggccgcg attggcgacc ggcgtgggtc gatgccaagg
     5521 gcctgaatga caagggccgc gcgatcgtcg aggaaatcaa ggcggcggac gattggggcc
     5581 ttgtcgcctc gaccttcgag ctgccgaacc tcgcgggtgc cggcgccgcc ttgggcaacc
     5641 aggtgatcgc cgccgaggat ctgaggctca cggtcgcgat cttgaaatac gcacgcgagg
     5701 cccgcggcgg caggaccgac ccgtcggtgc agctgtcgaa gaatctcgat cggacgccgg
     5761 cgctattcga accgagaagc gttctcgacg agacggcgac ctccgaggat ccgtcagcct
     5821 atctgcgccg ccttcatcct cagcacccgc agttcgaacg tctgcggaag gcctatgtcg
     5881 cactcaaggc gaagggccac gtcgcgatcg agaaaattcc ggacgggccg cgcatcgcgc
     5941 ccggcacttc gcaccctcac gtcgccatcg tccggcgccg gctcgggctc gcctcgctcg
     6001 gtgacgacgg ccgcgacatg ttcctcgatg aagccgtggc cagggcgctc cgtgagttcc
     6061 agtcggcgcg cggcatcacg cccgcctcca gtgccatcga tcttgccacg cgtcgcgcct
     6121 tcaacgcggt cgagggcggc agtccgaaac gcctacttgc caacatggag cagtggcgct
     6181 ggatgcccga cgatctcggg gccttccatg tctgggtcag cattcccgat tttaccgtgc
     6241 gcgtcatgaa ggatggccag gtgatctggt cggagcgcat catcgtcggc aaggtcgata
     6301 cgcagacgcc gatcttctcg gatgagatgg agttcgtggt cttccatccc gagtggggcg
     6361 tgccggattc catcaaggtc aaggagctct ggccgtcgct gcgccattcg accagcatcc
     6421 tcgccaagca gaacctgcgt gtcatgcaca atggtcgcgt gatcgatccc gattccgtcg
     6481 attgggcggg cgtcgacccg cggaccttct cgttcatcca gacgtccggc aacggcaacg
     6541 tgctcggcgt cgtgaagttc cgcttcccga acaagcacga cgtctacatg cacgacacgc
     6601 cgacgaaggg cctgttcagc tctgccgtgc gcgcctacag ccacggctgc atgcgcgtcc
     6661 agaaccccgt gcgtctcgcc gagatcgtgc tcgcccacga caagcagatg gccgccgagc
     6721 gcgttc
//
LOCUS       DAORNH010000256         6722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8426_length_6722_cov_2.394780, whole genome shotgun sequence.
ACCESSION   DAORNH010000256 DAORNH010000000
VERSION     DAORNH010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_6722_cov_2.394780"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK264_19975"
     CDS             complement(<1..762)
                     /locus_tag="PK264_19975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRD78184.1"
                     /translation="MLKAISSRLAGRAAVALPVAVALVASVPADASAGPALLFDPKSG
                     RVLYAEDPDHAWHPASVTKIMTAYLVFEALKAGRVTMATKITVSENALAQPPSKIGMP
                     VGTEVTIEFALQALIVKSANDVAVMLAEGVGGSEEAFVKLMNDRAKRLGMTRTNFVNP
                     HGLPAPEQVTTARDLAKLTRAVLTEFPEHAGYWSQPEVRVGRRRMRSHNTLLMTYEGA
                     DGIKTGFICDSGYNVVASATREGVKLVAIVLGEPSG"
     gene            1016..2164
                     /locus_tag="PK264_19980"
     CDS             1016..2164
                     /locus_tag="PK264_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78185.1"
                     /translation="MHFSLTEEQAAIQETALAFARDRLAPGAAEWDEKGFFPVDVIRE
                     TAALGMATIYVPAEKGGSGLSRLDGALIFEALATGCPAISSYISIHNMVAWMLARYGS
                     DDQAARWMPKLASMEWLASYCLTEPGAGSDAAALATTARRDGDHYVLDGVKQFISGAG
                     ATDLYFVFARTGGEGAKGVSAFILTRDMSGLSFGANERKMGWNAQPTRQVIMEGVRVP
                     AANLVGKEGEGFKFAMSGLDGGRINIGACSLGAAWTALDKARDYMSERKAFGQKLADF
                     QALQFKIADMATDLECARLMIYRAADALDRTDPQASMYSAMAKRVATDLGFKVCNEAL
                     QLHGGYGYLKDYGLEKLVRDLRVHQILEGTNEIMRLVVSRKVLGGNRG"
     assembly_gap    2284..2293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2294..3039)
                     /locus_tag="PK264_19985"
     CDS             complement(<2294..3039)
                     /locus_tag="PK264_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HRD78186.1"
                     /translation="MSGSVPSYGSQTHQSVPGASDDIDYAIRGQEMQFVEIELDPGES
                     ALAEAGSMMFKDAVVSMNTVFGDGTAANSGFMGKLKGLGRRMLTGESLFMTMFTHEGQ
                     GKAHAAFAAPYPGTILPFKLSEMGGMLICQKDAFLCAAKGVSIGIHLQRKIMTGLFGG
                     EGFIMQKLEGDGWAFVHVGGCVVERELGPGEELHVDTGCIACMTPTIEFEVVRAGNVK
                     SMIFGGEGLFFAQLRGPGKVWLQSLPFSRLP"
     gene            3526..3846
                     /locus_tag="PK264_19990"
     CDS             3526..3846
                     /locus_tag="PK264_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78187.1"
                     /translation="MPIAGYSSGLEMRSRSYDPQPKHPEAYWTLAVLLVLVVLSFIHP
                     LLVTFLSLICVAVGLVWAGLALLLPRDSGASVQRRLVAPGIVLLIGFAAGVIGDVDYL
                     MPAR"
     gene            3939..4646
                     /locus_tag="PK264_19995"
     CDS             3939..4646
                     /locus_tag="PK264_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HRD78188.1"
                     /translation="MLETVFAWFAMNYVALFNVIAIDVVLAGDNAIVVGLAASQVAPH
                     LRARVIFWGIAGAVVLRIIFAGLTSQLLQIIGLTLAGGLLLLWVCWKMYRQLQEHTKE
                     PRPHSLGAAAVSDDRATVQTVSFWAALWQIIVADVSMSLDNVLAVAGAAKGSPLVLII
                     GLVVAIVLMAVASHFIAGLLVRYPWITWLGLAIILYVAVDMIHDGSFEIACKGYNVSC
                     DTSLWSWATSSLFGSPK"
     gene            complement(4710..5678)
                     /locus_tag="PK264_20000"
     CDS             complement(4710..5678)
                     /locus_tag="PK264_20000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRD78189.1"
                     /translation="MTTAPIVDACQYCDWSEPIFRELREGNVAAISVTICYHEDFRET
                     VEAMIDWHRMIDQHSALLMRGRSAADIHEAQRLGKTAVFFASQNPSCIEDDIGLVEIL
                     NALGLKVMQLTYNNQSLLGSGCYEAADGGITRMGREVIREMNRVGMVIDMSHSGERST
                     FEAIELSARPIAITHANPKRWHDVARNKSDGLLKALAASGGMLGLSLYPLHLAGGSAC
                     TLESFTEMAARLADMIGVDHIGIGSDLCQGHGDGIIEWMRSGRWTKAPPALGTGKAVL
                     PPQPAWFRDNRDWEKIGSGLTARGFSAEDAGKILGGNWQRYFARSF"
     gene            complement(5712..>6722)
                     /locus_tag="PK264_20005"
     CDS             complement(5712..>6722)
                     /locus_tag="PK264_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD78190.1"
                     /translation="DGRTVTAKVILVATGGWPELPAIPGIELAITSNEAFHLPSLPKS
                     IVIVGGGYIAVEFAGIFNGLGVDTTLVYRREKILRGFDEDLREDLTGAMTKRGIKIVT
                     NATPTEISERASGFGVMNLRISLSDGREIDAGGIMFATGRAPNTKGLGLEAAGVALGK
                     RGEVKVDARSQSSVASIYAVGDVTDRANLTPVAIREGHAFADTVFGKKPWDADHELIP
                     TAVFSEPEIGTVGLSEADARAKFGTLDIYKARFRPMKYTLSGREERMLMKLVVDQRSQ
                     KVVGCHILGPDAGEIVQSVAIAMKMGATKADFDRTMALHPSAAEELVTMREKWVAPSK
                     SA"
BASE COUNT         1110 a         2215 c         2139 g         1248 t
ORIGIN      
        1 gcccgagggc tcgccgagca cgatggcgac gagcttcacg ccctcgcgcg tggcactcgc
       61 gacgacattg tagccgctat cgcagatgaa gcccgtcttg atgccgtcgg cgccctcgta
      121 ggtcatcagc agcgtgttgt gcgagcgcat gcggcggcgg ccgacgcgca cctcgggctg
      181 cgaccagtag ccggcgtgct cggggaactc ggtcagcacg gcgcgcgtga gcttggcgag
      241 gtcgcgcgcc gtcgttacct gctcgggcgc cggcagtccg tgcggattga cgaagttcgt
      301 ccgcgtcatg ccgaggcgct tcgcccggtc gttcatcagc ttgacgaagg cttcctcgct
      361 gccgccgacg ccctcggcga gcatcacggc gacgtcgttc gccgatttta cgatgagcgc
      421 ctgcagcgcg aactcgatgg tcacctcggt gccgaccggc atgccgatct tgctcggagg
      481 ctgggcgagc gcattctcgg agacggtgat cttcgtcgcc atcgtgacgc gtccggcctt
      541 caacgcctcg aagacgagat aggccgtcat gatcttggtc acggacgccg gatgccaggc
      601 gtggtcggga tcttcggcgt agagcacgcg gcccgacttc ggatcgaaca gtagcgcagg
      661 accagccgat gcatctgccg gcacactggc gactaatgcc acggcaacgg gcaaggcgac
      721 ggctgctcgc ccagcgagcc gtgacgaaat cgctttcaac atctggcctc tttcgacctt
      781 ctcgcgcatc gcgtgcgcca gccggcaccg tcgcaaccac cagtcccaca tgcttatgtc
      841 cctttcgcgg tcgcttgcaa agccgacaaa gtgtcgtgca cccgggcctg gtagatgcca
      901 aagcgtccgc tcgcgatccc gactgccgtg tgcagtctgg ttgcgcccct cgtgccggcg
      961 ctgctaatgg cgatcaccga gtcgcgcgct gtatcgacga agggtggagg acacgatgca
     1021 tttctcgctg accgaggagc aggccgcgat ccaggagacg gcgctcgcct tcgcacgtga
     1081 ccggctcgcg ccaggtgctg ccgagtggga cgagaagggc tttttccccg tcgacgtcat
     1141 tcgcgagacg gcggccctcg gcatggcgac gatctatgtc ccggccgaga agggcggctc
     1201 aggtctttcg cggctcgacg gcgcgctcat attcgaggcg ctcgcaacgg gctgcccggc
     1261 gatctcgtcc tacatctcga tccacaacat ggtcgcctgg atgctggccc gctatggctc
     1321 cgatgaccag gccgcccgct ggatgccgaa gctcgcctcg atggagtggc tcgcctccta
     1381 ctgcctgacg gagccgggtg ccggctcgga tgcggctgcc ctcgcgacca ccgcccgccg
     1441 cgacggcgat cactatgtgc tcgacggtgt caagcagttc atctcgggcg caggtgcgac
     1501 cgacctctac ttcgtcttcg cgcggaccgg cggcgagggc gccaagggcg tctccgcctt
     1561 catcctgaca agggacatgt cgggcctctc gtttggcgcc aacgagcgaa agatgggttg
     1621 gaatgcgcag ccgacgcggc aggtgatcat ggagggcgtg cgtgtccccg ccgccaatct
     1681 cgtcggcaag gaaggcgagg gcttcaagtt cgccatgagc ggcctcgatg gcgggcgcat
     1741 caacatcggc gcctgttcgc tcggcgcggc ctggaccgcg ctcgacaagg cgcgcgacta
     1801 catgagtgag cgcaaggcct tcggccagaa gctcgccgac ttccaggcgc tccagttcaa
     1861 gatcgccgac atggcgaccg atctcgagtg cgcgcgcctc atgatctatc gcgcggccga
     1921 cgccctcgat cgcaccgacc cgcaagcttc catgtactcg gccatggcaa aacgcgtcgc
     1981 gaccgacctc ggcttcaagg tctgcaacga ggcactgcag ctgcacggcg gctacggcta
     2041 cctgaaagac tacggcctcg agaagctcgt gcgcgacctg cgcgtccacc agatcctcga
     2101 gggcaccaac gagatcatgc gcctcgtcgt ctcgcgcaag gtactcggcg gcaaccgcgg
     2161 ctgactccgc cgcggcgccg cgcctccggt ttcaccccca gcggcgtcag ttgttgtcgc
     2221 cctggaagag cccgccgatg atggaaccct cgcccttgct gccaccggcc gccatcagca
     2281 tgcnnnnnnn nnngggagcc gcgagaacgg caggctctgc agccaaacct tgccggggcc
     2341 ccgcagctgc gcgaagaata gaccttcgcc gccgaatatc atcgacttca cgttgcccgc
     2401 gcgtacgacc tcgaactcga tggtcggcgt catgcaggcg atgcagcccg tatcgacatg
     2461 caactcctcg cccgggccga gctcgcgctc gacgacgcag ccgccgacgt gcacgaaggc
     2521 ccaaccgtcg ccctcgagct tctgcatgat gaagccttcg ccgccgaaca ggcccgtcat
     2581 gatcttgcgc tgcaggtgga tgccgatcga gacgcccttg gcggcgcaca ggaacgcgtc
     2641 cttctggcag atcagcatgc cgcccatctc ggagagcttg aagggcagga tggtgccagg
     2701 atagggcgcc gcgaacgccg cgtgcgcctt gccctgaccc tcgtgcgtga acattgtcat
     2761 gaagaggctc tcgccggtga gcatccgccg gccgaggccc ttgagcttgc ccatgaagcc
     2821 ggagttggcg gcggtgccgt cgccgaacac cgtgttcatg ctcaccacgg cgtccttgaa
     2881 catcatggag ccggcttccg cgagcgcgct ctcgcccgga tcgagctcga tctcgacgaa
     2941 ctgcatctcc tggccgcgga tggcgtaatc gatatcgtcc gaggcgcctg gaacggactg
     3001 gtgcgtctgg ctgccgtagc tcggaacaga acccgacatg tcggtctcca tgatcaatga
     3061 agtctgtcgt acagccggct cggaactcga tcttgagcga gccggcgagg acagagcttt
     3121 catagcagaa aacgatcggc cgcgcctgcg tcgtgtagcc ggtcctgttg gacgccggcg
     3181 cagtcgcggg tcgccgccgc tggcgctgtc accaccatgc ctccggcgac aggcgaacgc
     3241 ggtcgcgcgc agccctccca ccagcttcga agtacgcgcc atgccgatgc atcggaaacg
     3301 tcaattcgac gcatcaattt tggcgatgga attctccgtt gctgccacga tcagtaacat
     3361 cgaaaattcc aattgtggaa attgaaaaga ccaataattg atcatagtgc catcctatca
     3421 attcaacggg ctatttctat ttgagcgcgg aaagcctcgg gcggatgctt gatcgccgag
     3481 ccgactaatg gaaatgacgc tccaacgacc gagaggaggg cggaaatgcc gatcgccggg
     3541 tattcatcgg gcctcgaaat gcggagccga tcctacgatc cgcagccgaa gcatccagag
     3601 gcgtattgga cgctggcggt gctgttggtg ctcgtcgtcc tctcgttcat ccatcccctc
     3661 ctcgtcacgt tcctcagcct catctgcgtg gctgtcggcc tcgtctgggc aggcttggcc
     3721 ctgctgctcc cgcgcgattc gggcgccagc gtgcagcgtc ggctcgtggc acctggcatc
     3781 gtgctcctga tcgggtttgc cgccggcgtc atcggcgatg tggactacct catgcctgca
     3841 cggtagcggc cgagctgaaa cttcgacgtg agacgcgcgg gcatggccga tggctgcccg
     3901 cgcaatctcc ttgatgcctc aatcagcgga acactcgaat gctcgagacg gtctttgcat
     3961 ggttcgccat gaattacgtg gcgttgttca acgtgatcgc catcgacgtc gtcctggccg
     4021 gcgacaatgc gatcgtcgtc gggctggccg cttcgcaggt cgcacctcac ttgcgggcac
     4081 gcgtcatttt ctggggcatc gccggcgcgg tcgtcctccg gatcattttc gcgggcctga
     4141 cctcccagct cctgcaaatc atcggcttga cgttagcggg aggcttgctc ctgttgtggg
     4201 tctgctggaa gatgtatcgc cagctgcagg agcatacgaa ggagccccgg cctcattcgc
     4261 tcggtgcggc ggcggtgtcg gatgaccgcg ccactgtcca gaccgtgagc ttctgggccg
     4321 ctctctggca gatcatcgtc gctgatgtgt cgatgtcgct cgataacgtg ctcgccgtcg
     4381 ccggtgcggc gaagggttcg cccctggtgc tgatcatcgg tcttgtcgtg gccatcgtcc
     4441 tcatggccgt cgcatcgcac ttcatcgccg gcctgctcgt ccggtatcct tggatcactt
     4501 ggctcggtct cgcgatcatc ctctacgttg ccgtcgacat gattcacgac ggcagcttcg
     4561 agatcgcctg caagggctac aatgtgagct gcgatacgag cctgtggagc tgggccacca
     4621 gcagtctgtt cggatcgcca aagtagagct ggctgccgac ctatgcacct gacgcgccgt
     4681 tcgccgacga ggcggacgtc gcagccgtgt cagaacgatc gcgcaaagta gcgctgccag
     4741 ttgccaccga ggatctttcc ggcatcctcg gcggaaaatc cgcgcgcggt gaggcccgaa
     4801 ccgatcttct cccagtcgcg gttgtcgcgg aaccaggcgg gttggggcgg tagcacggcc
     4861 ttccccgtgc cgagggccgg cggcgccttc gtccagcggc ctgagcgcat ccactcgatg
     4921 atgccgtcgc cgtgtccctg gcagaggtcc gagccgatgc cgatgtggtc gaccccgatc
     4981 atgtccgcga gccgcgccgc catctccgtg aagctctcga gcgtgcacgc cgagccgccg
     5041 gcgagatgca gcggatagag cgagaggccg agcatgccgc ccgaggccgc gagcgccttc
     5101 aggagcccgt cggacttgtt gcgcgcgacg tcgtgccagc gtttcgggtt ggcgtgcgtg
     5161 atggcgatcg gccgggcgga cagctcgatc gcctcgaagg tcgagcgttc gcccgaatgc
     5221 gacatgtcga tcaccatgcc gacgcggttc atctccctga tcacctcgcg gcccatccgc
     5281 gtgataccgc cgtcggccgc ctcatagcag ccggagccca gcagcgactg attgttgtag
     5341 gtgagctgca tcaccttgag gccgagcgcg ttcaggatct cgaccaggcc gatgtcgtcc
     5401 tcgatgcagg aggggttctg cgaggcgaag aacaccgcgg ttttaccgag ccgctgagcc
     5461 tcgtggatgt cggcggccga gcgcccgcgc atcaggagag cgctgtgctg gtcgatcatg
     5521 cgatgccagt cgatcatcgc ctcgacggtc tcgcggaagt cctcatggta gcagatcgtc
     5581 accgagatgg cggcgacatt cccttcgcgg agctcgcgga agatcggctc ggaccagtcg
     5641 cagtactggc aggcatcgac gatgggggcg gtggtcacgg cagcggcctc gggttctgaa
     5701 aggtcgggcg atcaggcaga cttggagggc gcaacccact tctcacgcat ggtgacgagt
     5761 tcttctgcgg cactcgggtg cagcgccatg gtgcggtcga agtcggcctt ggtcgctccc
     5821 atcttcatgg cgatggcgac gctctggacg atctcgccgg catccggccc caagatgtgg
     5881 cagccgacga ccttctgcga tctctgatcg acgacgagct tcatgagcat gcgttcctcg
     5941 cggccggaga gtgtgtactt catcggccgg aaacgcgcct tgtagatgtc gagcgtaccg
     6001 aacttcgcgc gtgcgtctgc ctctgaaagc ccgaccgtgc cgatctcggg ctccgagaac
     6061 accgccgtcg ggatcagctc gtgatcggcg tcccacggct tcttgccgaa gaccgtgtcg
     6121 gcgaaggcat ggccttctcg aatggcgacg ggcgtgaggt tcgcccgatc ggtgacatcg
     6181 ccgaccgcat agatgctcgc gacgctgctc tgcgatcgcg cgtcgacctt cacctcgccc
     6241 cgcttgccga gtgcaacgcc cgccgcttca agtccgagcc ccttggtgtt cggcgctcgc
     6301 ccggtcgcaa acatgatgcc gcccgcgtcg atctcacggc cgtcggacag actgatgcgg
     6361 agattcatga cgccgaagcc cgatgcccgt tcgctgattt cggtcggggt cgcgttggtg
     6421 acgatcttga tgccacgctt cgtcatcgcg ccggtgaggt cttcgcgcag gtcctcgtcg
     6481 aagccacgca ggatcttctc gcgccggtag acgagcgtcg tgtcgacgcc gagcccattg
     6541 aagattccgg cgaactccac cgcaatgtaa ccaccgccaa cgatcacgat cgacttcggc
     6601 aagctcggca aatggaaggc ctcattcgag gtgatcgcaa gctcgatgcc tggaattgcg
     6661 ggcaactcgg gccagccacc ggtcgccacc aggatgacct tggccgtcac cgtgcgtccg
     6721 tc
//
LOCUS       DAORNH010000257         6716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8437_length_6716_cov_2.341240, whole genome shotgun sequence.
ACCESSION   DAORNH010000257 DAORNH010000000
VERSION     DAORNH010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8437_length_6716_cov_2.341240"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK264_20010"
     CDS             complement(<1..772)
                     /locus_tag="PK264_20010"
                     /inference="COORDINATES: protein motif:HMM:NF018420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HRD78191.1"
                     /translation="MTRALAWLAVAILTALVGSADALRAQAPGSAPPGSRIEVDLELV
                     LAVDISYSMDPEEQRLQRDGYVLAFRDKALWAAIAGGKNQRIAVTYVEWAGPPIQSII
                     LPWTLVDGPAAAEKVAALLSAQPISRGRMTSITGILEFSDALFGTAGFKGLRRTVDIS
                     GDGPNNAGGLVTTARDKLVAKGIVINGLPVMLKLGQSGFFDIVDLDEYYTECVIGGAG
                     SFVIPIRSREEFVSATRQKLILEIAGVEPSFYIRLAAGN"
     gene            914..1255
                     /locus_tag="PK264_20015"
     CDS             914..1255
                     /locus_tag="PK264_20015"
                     /inference="COORDINATES: protein motif:HMM:NF021125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HRD78192.1"
                     /translation="MALKRMVLEIGMGTDIRGRDSTKAAVRALKDALWRNALTIAQAL
                     DLEPKDMHVKVLIGVPRPETVDRAEVLAALPYGGGEIEVHEGGLFVPRPDGTDGTTIA
                     HAAAIVMLDVA"
     gene            1266..1622
                     /locus_tag="PK264_20020"
     CDS             1266..1622
                     /locus_tag="PK264_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HRD78193.1"
                     /translation="MTRKRIIVELGAGTALHGGNYTKAAVRAVQDAIHHSSMSFLKSL
                     RIPAAEMEVEVTIGVQRPDMVDAEAVRTALPHGRIIVKVVKGGLDVPDPEAADLCVIA
                     SAAVAVFVDMAKRGTA"
     gene            1741..2922
                     /locus_tag="PK264_20025"
     CDS             1741..2922
                     /locus_tag="PK264_20025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HRD78194.1"
                     /translation="MIEKAVNYDDLRRMAKRFMPKVAFDFVEGGVEDEDGVERNKQAF
                     RNVRLVPKYMVDVSTRDQKTEIFGRTYSHPYGIAPTGGLAFYRQGSDLMLAAAARDAN
                     VPFIMSGTSTASIEDLARTAPDHGWYQLYTAKDKSISEDQIARARDAGLSTLVITVDV
                     PVSSNRERNKRNGYGRPLNLPMRTKLETLLHPEWLVEYMQKGKPFISNWAKYAKDPKD
                     VDAVAELVASQVQATLTWDDLKRFRDLWPRKLVLKGVMHPDDAKRAQSVGIDGLMVSN
                     HGARQLDRAPSPVEVLPAIVDAVGEKMTVMLDSGVRRGSDVIAALCLGAKFVFVGRWT
                     LYGVAAGALPGAKRALEMIRNEVDLQMGQMGAPDIKSLGPDFVLWDDIEDLRRNRRPV
                     R"
     gene            3144..4100
                     /locus_tag="PK264_20030"
     CDS             3144..4100
                     /locus_tag="PK264_20030"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HRD78195.1"
                     /translation="MALLSFVGLGVRPATNRAALAGRGSRSGKPAIAIVALISLAASL
                     PQATAQVVDAAVTKAAATSRAAARVEWPSQVHASYQVAFNGMDVGRFDFQSSITGSSY
                     TLTGNAELSALLGAFSWRGATRSFGTIAGENARPQSYLFDFAGTGKSGSLKMSFADNA
                     VSGVTAVPTIPPTADTVPLTLAHLKGALDPLTAVIALSRGGTSPCARRVPVFDGKQRF
                     DLVLSYKGQEGVREAQPSGQPGVAIVCRVKYVPIAGHKLNAETRAMAESTGIEIAFRP
                     VPTAGLFVPHRITIPTGAGTASLTSTRISIVTAKRNQIAFVH"
     gene            4297..>4650
                     /locus_tag="PK264_20035"
     CDS             4297..>4650
                     /locus_tag="PK264_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRD78196.1"
                     /translation="MDLESRIRNVTAVLGPTNTGKTHLAIERMLGHESGLIGLPLRLL
                     AREVYDKIVQRVGSEAVALITGEEKIKPERARFSVCTVEAMPRDVDVDFLAIDEVQLA
                     ADPDRGHVFTDRLLHS"
     assembly_gap    4651..4671
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4672..>4993
                     /locus_tag="PK264_20040"
     CDS             <4672..>4993
                     /locus_tag="PK264_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRD78197.1"
                     /translation="LLGAGTMRDAIADLIPGANFISRPRLSKLTYSGEKKITRMPART
                     AIVAFSANEVYEIAELIRRQRGGAAVVLGALSPRTRNAQVALYQGGDVQYLVATDAIG
                     MGLNL"
     assembly_gap    4994..5049
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5050..>5911
                     /locus_tag="PK264_20045"
     CDS             <5050..>5911
                     /locus_tag="PK264_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRD78198.1"
                     /translation="KLTAAELGQIAGRAGRHMNDGTFGVTGDVRPFDADLIERLENHE
                     FEPVKLLQWRNRRLDFGSVDRLRESLKDLPRESRLTRARVADDVMALEALSGDAEVVK
                     RARSAAEVELLWEVCQVPDYRKISTQNHAELVTSLFMHVSSGEGKIPEDWFSRQVDLA
                     NRTDGDIDTLATRLAHIRTWTFVSNRASWLKDPAHWQAKTREIEDALSDALHEQLTQR
                     FVDRRTSALIRGMRDKEELAAEIGDDGAIRVEDHYVGRLKGFRFQPDTQSEDIHGKAS
                     RSAAGQVLAKE"
     assembly_gap    5912..5921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5922..>6340
                     /locus_tag="PK264_20050"
     CDS             <5922..>6340
                     /locus_tag="PK264_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRD78199.1"
                     /translation="VAAAKPDAFKLARNGRILWRDDEIAEVQPSEDPLKPTVTLLVDE
                     HISGPDKEKVQERLNQWIGEVVADKLAPLVELAKAEDISGLARGIAFRLQESFGILKR
                     EEVGEEIKSLDQAARGQLRKYGVRFGAFNIYIPLLLK"
     assembly_gap    6341..6394
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6395..>6716
                     /locus_tag="PK264_20055"
     CDS             <6395..>6716
                     /locus_tag="PK264_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HRD78200.1"
                     /translation="GMSLEALPEPPRAGLTSLVPDKAVPEAYYRVLGYHHCGPRAIRV
                     DMLERLADAIRPLLAFRSNASGNPPPKGSTGDGGFTAIPEMMSILGCSSAELGEVLKA
                     LGFRS"
BASE COUNT         1115 a         2272 c         2119 g         1069 t
ORIGIN      
        1 agttgccggc cgcgaggcgg atgtagaacg agggctcgac gccggcgatc tcgagaatga
       61 gcttctggcg ggtcgccgac acgaactcct cccgcgagcg gatcgggatc acgaacgagc
      121 cggcgccgcc gatcacgcac tccgtatagt attcgtcgag atcgacgata tcgaagaacc
      181 cggactggcc gagcttcagc atcaccggaa ggccgttgat gacgatgccc ttggcgacga
      241 gcttgtcgcg tgccgttgtg acgaggccgc ccgcattgtt ggggccgtcg cccgagatgt
      301 cgaccgtgcg cctgagcccc ttgaagccgg ccgtaccgaa cagcgcatcc gagaattcga
      361 ggatgcccgt gatcgaggtc atccggccgc gcgagatcgg ctgggccgag agcagggcgg
      421 cgaccttctc ggccgccgcc ggtccgtcga ccagcgtcca cggcaggatg atgctctgga
      481 tcggcgggcc ggcccactcg acgtaagtca cggcgatgcg ctggttcttc ccgccggcga
      541 tggcggccca cagcgccttg tcgcgaaacg cgagcacgta gccgtcgcgc tgcaggcgct
      601 gctcctccgg gtccatcgag taggagatgt cgaccgcgag cacgagctcg agatcgacct
      661 cgatccggct tccgggagga gccgaacccg gagcctgggc acgcagcgca tccgcgctgc
      721 cgacaagtgc ggtcaggatg gcgaccgcga gccatgcaag tgcgcgtgtc atgcgggaaa
      781 ggtgcgccgc acctgtggct ttggcaaggc acttttgcgc gagcggaaat gcgtgatggg
      841 cgattgccgc ggcggccggc ccggtgcaaa cgtctcgcgg gtcgagcggc gcgggttccg
      901 gctggaggtg ttcatggcat tgaagcgaat ggtgctcgag atcggcatgg ggacggacat
      961 ccgcggtcgc gacagcacca aggccgcggt gcgtgcgctg aaggacgcgt tgtggcggaa
     1021 cgcactgacg atcgcccagg ccctcgacct cgagccgaag gacatgcacg tcaaagtcct
     1081 gatcggcgtg ccgcgccccg agaccgtcga tcgtgccgag gtgctggccg cgctccccta
     1141 tggcggcggc gagatcgagg ttcacgaggg cggcctcttc gtgccgcgcc ccgacggaac
     1201 cgatggcacg accatcgcgc atgccgcggc gatcgtcatg ctggatgtgg cgtgaggagc
     1261 cccgaatgac cagaaaacgc atcatcgtcg agctcggcgc cggcacggcc ttgcacggcg
     1321 gcaactacac caaggcagct gtacgcgcgg tccaggacgc gatccatcac agctcgatgt
     1381 cgttcctgaa atcgctgcgc attcccgccg ccgagatgga ggtcgaggtg acgatcggcg
     1441 tccagaggcc cgacatggtc gatgccgagg cggtacgcac ggcgctgccg cacgggcgga
     1501 tcatcgtgaa ggtcgtcaag ggcgggctcg atgtgccgga tcccgaggct gccgacctct
     1561 gcgtcatcgc cagtgccgcc gttgccgtct tcgtcgacat ggcgaaacgc ggcacggcct
     1621 gaaaccacgg cacgcgatca ccgcttgatt gcggcgcgga gccgctgcaa cgtgcgatcc
     1681 gagttctcac ccgagccgcc gaggcggcaa caatcccgac atttcgtcct ggagcgtccg
     1741 atgatcgaga aggcggtgaa ctacgacgac ctgcgccgca tggccaagcg cttcatgccg
     1801 aaggtcgcct tcgacttcgt cgagggcggc gtcgaggacg aggacggcgt cgagcgcaac
     1861 aagcaggcct tccggaacgt gcgcctcgtg ccgaaataca tggttgacgt ctcgacacgt
     1921 gaccagaaga ccgaaatctt cggtcgcacc tactcgcacc cttacggcat cgcgccgacc
     1981 ggcggcctcg ccttctaccg gcaaggttcc gatctgatgc ttgccgccgc cgcccgcgat
     2041 gccaacgtcc ccttcatcat gtccggcacg tcgacggcct cgatcgagga cctcgcccgc
     2101 acggcgcccg atcacggctg gtaccaactc tatacggcaa aggacaaatc gatttcggag
     2161 gaccagattg cgcgcgcgcg cgatgccggc ctctcgacgc ttgtcatcac cgtcgatgta
     2221 ccggtctcct cgaatcgcga gcgcaacaag cgcaacggct acggccgacc gctcaatctg
     2281 cccatgcgca cgaagctcga gacgcttctg caccccgaat ggctcgtcga gtacatgcag
     2341 aagggcaagc ccttcatctc caactgggca aagtatgcca aggacccgaa ggacgtggac
     2401 gccgtcgccg agctggtcgc gagccaggtg caggctacac tcacctggga cgacctcaag
     2461 cgcttccgtg acctttggcc acgcaagctg gtgctgaagg gcgtcatgca ccccgacgac
     2521 gccaagcgcg cccagtcggt cggcatcgat ggcctgatgg tctcgaacca cggcgcccgc
     2581 cagctcgacc gcgccccctc gccggtcgag gtgctgccgg cgatcgtcga cgccgttggc
     2641 gagaagatga cggtgatgct cgacagcggc gtccggcgcg gctccgacgt aatcgcggca
     2701 ctctgcctcg gcgccaagtt cgtattcgtc ggccgctgga cgctctacgg cgtcgcagcc
     2761 ggcgcgcttc cgggcgccaa acgcgcgctc gagatgatcc gcaatgaggt cgacctccag
     2821 atgggccaga tgggcgcgcc cgacatcaag tcgctcgggc ccgacttcgt gctctgggac
     2881 gatatcgaag accttcgccg caatcgtcgc ccggtgcgct gatctccttg ccgcagtccc
     2941 gcttttcaaa tggccgggcg caccgcaatg gcgcgcccgt aaaacgtttg ttagcttttc
     3001 cgggcgactc tgtccgcgac acacaaacga cgaattgcct gcctacccgt tcgatgacga
     3061 gggagcgagg cgagtttctg ttctgcgtgt gtcgcgcgcg tcgaggtgcg cttggttcag
     3121 ggtctcgtga gtaaaggtcc gtcatggcgt tgttgtcgtt cgtcggtctc ggcgtgaggc
     3181 ctgccaccaa tcgtgccgcg cttgcgggtc gcggctccag gtccggcaag ccggcgattg
     3241 cgatcgtggc gctgatctcg ctcgccgcct ccctgcctca ggcgaccgcg caggtggtcg
     3301 atgccgccgt caccaaggcc gctgccacgt cgcgtgccgc cgcgcgggtc gaatggccta
     3361 gtcaggtgca cgcgagctac caggtcgcct tcaacggcat ggatgtcggc cgcttcgact
     3421 tccagtcgtc gatcaccggc tcaagctaca cgctcacggg caatgccgag ctctccgcct
     3481 tgctcggtgc cttctcctgg cgcggcgcga cccgctcctt cggcacgatc gccggcgaga
     3541 acgcgcggcc gcagagctac cttttcgatt ttgccgggac cggcaagtcc ggctcgctca
     3601 agatgagctt tgccgacaac gccgtctccg gcgtcaccgc ggtgccgacg atcccgccga
     3661 ccgccgatac cgtcccgctc acgctcgcgc acctcaaggg agcgctcgat ccgctgaccg
     3721 ccgtgattgc gctgtcgcgc ggcggcacct cgccttgcgc ccgccgcgtc cccgtattcg
     3781 acggcaagca gcgcttcgat ctggtgctga gctacaaggg tcaggaaggt gtccgcgagg
     3841 cgcagccctc gggccaaccg ggcgtcgcga tcgtctgccg cgtgaaatat gtgccgatcg
     3901 ccggccacaa gctcaatgcc gagacgcgtg ccatggccga gagcaccggc atcgagattg
     3961 cgttccgccc cgtcccgaca gccgggctct tcgtgccgca ccgcatcacc atcccgaccg
     4021 gagcgggcac ggcatcgctt acctcgacac gcatctcgat cgtcacggca aagcgcaacc
     4081 agatcgcctt tgtgcactga cggtcagaca cacctcattt gcaaccgcgc cgatccgtgg
     4141 atcggcgcgg tttcgttttg ggcgtcgaag tgcgcgatcc gggcagaccc gcgcgccgcc
     4201 cgccactttt ccgccatcgg ttgcgcttga tctgtcggcg aagctctgcg atgcgtctcc
     4261 atcgcgggca caaagtcagt acctaggggg cagtccatgg atctcgagag ccgcattcgc
     4321 aatgtgacag cggtgctcgg accgacgaac acgggcaaga cgcatctcgc catcgagcgg
     4381 atgctcgggc acgaaagcgg tctcatcggc ctgccgctgc gcctcctggc gcgcgaggtc
     4441 tacgacaaga tcgtccagcg tgtcggcagc gaggccgtgg cgctcatcac cggcgaggag
     4501 aagatcaagc ccgagcgggc gcgcttctcc gtctgcacgg tcgaggccat gccgcgcgat
     4561 gtcgacgtcg atttccttgc gatcgacgag gtgcagctcg ctgccgaccc cgatcgaggc
     4621 catgtcttca ccgaccggct cctgcattca nnnnnnnnnn nnnnnnnnnn nctgctcggc
     4681 gccggcacca tgcgcgatgc gatcgccgac ctcattcccg gcgcgaactt catctcgcgg
     4741 ccgcgcctct cgaagctcac ctattcgggt gagaagaaga tcacgcggat gcccgcacgc
     4801 acggccatcg tcgctttctc cgcgaacgag gtctacgaga tcgccgagct gattcgccgc
     4861 cagcgcggcg gggcggccgt cgtgctcggc gccctctcgc cacggacgcg caacgcccag
     4921 gtcgccctct accagggcgg cgacgtgcaa tacctcgtcg cgaccgacgc catcggcatg
     4981 ggcctcaatc tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnna agctcaccgc cgccgagctc ggccagatcg ccggccgcgc cggccgccac
     5101 atgaacgacg gcaccttcgg cgtgacgggt gacgtccgcc ccttcgatgc cgaccttatc
     5161 gagcgcctcg agaaccacga gttcgagccc gtgaagctcc tgcagtggcg caaccggcgg
     5221 ctcgacttcg gctcggtcga ccggctgcgc gagagcctga aggatctccc ccgcgaaagc
     5281 cggctgacgc gcgcccgcgt cgccgacgac gtgatggcgc tagaggcctt gagcggggac
     5341 gccgaggtcg tcaaacgcgc gcgctcggcc gctgaggtcg agctgctgtg ggaggtctgc
     5401 caggtccccg actaccgcaa gatttcgacg cagaaccacg ccgagctcgt cacctcactc
     5461 ttcatgcatg tctcgtccgg cgagggcaag atccccgaag actggttctc ccgccaggtc
     5521 gatctcgcca accgcacgga cggcgacatc gacacgctcg cgacgcgcct tgcccacatc
     5581 cgcacctgga cctttgtctc caatcgcgcg agctggctca aggatccggc gcactggcag
     5641 gcgaaaaccc gcgagatcga ggacgcgctt tccgatgcgc tacatgagca gctgacgcag
     5701 cgcttcgtcg accggcgcac gagcgcgctc atccgcggca tgcgcgacaa ggaggagctc
     5761 gccgccgaga tcggcgacga cggcgcgatc cgcgtcgagg atcactacgt cggccgcctc
     5821 aagggcttcc gctttcagcc cgacacgcag tccgaggaca tccacggcaa ggcgtcgcgc
     5881 tcggccgcgg ggcaggtgct cgcgaaggaa cnnnnnnnnn ngggtcgccg cggcgaagcc
     5941 cgatgccttc aagctcgcac gcaacggccg cattctctgg cgtgacgacg agatcgccga
     6001 ggtgcagcct tccgaagacc cgttgaaacc gacggtgacg cttctcgtcg acgagcacat
     6061 ctcgggtccc gacaaggaga aggtgcagga gcggctcaac cagtggatcg gcgaagtcgt
     6121 cgccgacaag ctcgcgcccc tcgtcgagct cgccaaggcc gaggacatct cggggctcgc
     6181 tcgaggcatc gccttcaggc tccaggagag cttcggcatc ctgaagcgtg aggaggtcgg
     6241 cgaggagatc aagtcgctcg accaggccgc ccgcggccag ctgcgcaagt acggcgtccg
     6301 cttcggcgcc ttcaacatct acatcccgct gctgctaaag nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggcat gtcgctcgag gccttgcccg
     6421 agccgccgcg cgccggactg acttcgctcg tccccgacaa ggcggtaccg gaagcctact
     6481 atcgcgtgct cggctaccat cactgcggcc cgcgcgcgat ccgcgtcgac atgctcgagc
     6541 gtctcgccga cgccatccga cctctgctcg cgttccgctc caacgcatcc ggcaatccgc
     6601 caccgaaggg ttcgacgggc gacggcggat tcacggccat ccccgagatg atgtcgatac
     6661 tcggctgctc gtcagctgag cttggcgagg tcttgaaggc actgggattc cgatcg
//
LOCUS       DAORNH010000258         6656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8536_length_6656_cov_2.775337, whole genome shotgun sequence.
ACCESSION   DAORNH010000258 DAORNH010000000
VERSION     DAORNH010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_6656_cov_2.775337"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>413
                     /locus_tag="PK264_20060"
     CDS             <1..>413
                     /locus_tag="PK264_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRD78201.1"
                     /translation="LGHVASRPPINVAFEAGPNAANVFQPACTLCGDCCSGCNVGAKT
                     TVQMSYLPDAVNHGAEVFTEVTARHVKKLGTSHGHTWQVFYVPTQDHREGFRQVERAI
                     SARIVVLAAGALGSTELLLRSREEGLAVSDRLGSR"
     assembly_gap    414..423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <424..2964
                     /locus_tag="PK264_20065"
     CDS             <424..2964
                     /locus_tag="PK264_20065"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HRD78202.1"
                     /translation="ISGDPLQTQAQVLAFGYNNDRAVNAVGVGHPARRGVSPPGPCIA
                     GAIDLRDQSHLEEGMIIEEGVLPSGLAPLLPGIFSAGFHLTGKDTDQGLADGALEAAR
                     RAKSWVLGAYWGAMQHTETFLVMAHDDAGGRIELDDGRLTVRWPGVAEQPIYRTIEAT
                     LTKATAATGGAFVRNPLQSTQIVGKTLVTVHPLGGCPMGADASRGVVDHACRVFDPAG
                     GVHEGLLVCDGAVMPRPLGVNPLFTISAIAERAMIELARRENRPLSDAPRADARLRKL
                     VGDEDDRRPAGVEFTERMAGFLSTASADYQSGMAAGKSAGTEFAFTATIRVDDIARFE
                     SDPEHAGRISGTALCPQLSADPLDIADGRFNLMRPDPTRVETRRFDYQMQLISRDGRR
                     FRFAGHKVVRADAGVTDLWRDTTTLFVDLVEETGTGATAKGILTIAATDFARQLTTLK
                     GTGGGSIKERLSAVGRFGGVFAGTLFDVYGGLAAPLKRYDGTIVRKKRALRTPHPEVH
                     EFAALDGRKLRLTRYHGGDKGPLLFSHGLGVSSLIFSIDTIDTNLLEFMVAAGYDCWL
                     LDFRASTDLPHARDRWTADDCATKDYQPAVDLIREKSGATSVQVIAHCFGATTFVMAM
                     LSGLKGVRSAVISQIATDVIVPFWPQRALAYLRTPSLFSLLGIGHVNARATKEAGVAE
                     RLMDGLIRIAVPFQRDERTRNATSNRITALYGQLYETAQLDADTFEFGLAEMFGPANI
                     DAFKQLALIARRQVIVNAAGEDVYLPHLERLAIPITIIHGANNACFRPESTVRTLERL
                     TKRNGASFYQRHEIAGYGHIDCIFGKRAAVDVYPIMLAHFEPTALHRV"
     gene            complement(2993..4300)
                     /gene="fahA"
                     /locus_tag="PK264_20070"
     CDS             complement(2993..4300)
                     /gene="fahA"
                     /locus_tag="PK264_20070"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HRD78203.1"
                     /translation="MALNETHDPALKSWVASANVPGCPFPIQNLPFCIFSAPGRTRRM
                     GVGIGDQILDLSATFEAGHLRAGHIGAEIFPQPSLNAFMATGRTIWREMRGHISRFLD
                     ARNPADKTIDRALHEKILVPMSAASLHLPIEVRSFTDFYASKEHATNVGAMFRGPENA
                     LMPNWLHIPIGYNGRASTVVVSGTDIRRPLGQTKAPDAAMPSFGPCKRLDFELELGAI
                     VGTGNRMGEPVTVAEAYDMIFGYVLLNDWSARDIQAWEYQPLGPFQSKAFATSISPWV
                     VTAEALEPFRVATPERVKPLLPYLVERGANNFDLKLEVALTPAGSNRPTTISRTNYKG
                     MYYSAAQQLAHHAVGGCEMCTGDLLGSGTISGPTKDSLGSLVEITWGGKEPLGLEGGA
                     KRTFLEDGDELVLTGHCEKDGVRIGFGECRGKVLPAPKEREWG"
     gene            complement(4324..5664)
                     /gene="hmgA"
                     /locus_tag="PK264_20075"
     CDS             complement(4324..5664)
                     /gene="hmgA"
                     /locus_tag="PK264_20075"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HRD78204.1"
                     /translation="MRPARDSASSIAEGNRGYMSGFGNGFESEALPGALPVGRNSPQK
                     CPYGLYAEQISGSPFTAPRVSNERSWLYRIRPTVKHWGQFKKADAGLWRTAPCTEVEV
                     PPAPMRWDPPALPKGSVSFIEGIRTMTTCGDAGTLGGMGANVYLVTRSMKDEYFYNAD
                     GEMMFVPQQGRLRIRTEFGVIDIEPAEIAVIPRGVKIAVELNGDPAARGYLCENYGAA
                     FTLPERGPIGANCMANQRDFLTPVAAYEDRDQPCRLFVKWGGTLWAAEIGHSPLDVVA
                     WHGNFAPYKYDLRHYSPVGPILFDHADPSIFTVMTSPSETPGTANIDFVVFSDRWMVA
                     ENTFRPPWYHLNVMSEFMGLVYGAYDAKPHGFVPGGMSLHNSMLPHGPDTEAFEKASN
                     VKLEPHKQQNTLAFMFETRFPQRVTRYAAEQAGLQGDYVDCWHGIKKHFDPKKR"
     gene            complement(5775..>6656)
                     /gene="hppD"
                     /locus_tag="PK264_20080"
     CDS             complement(5775..>6656)
                     /gene="hppD"
                     /locus_tag="PK264_20080"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HRD78205.1"
                     /translation="HAARFVAEHGPCAPAMAWRVIDAHHALARALSLGASEYTGSDKS
                     IDAPALVGIGGSLLYLVETYGAKGSPYARDYDWLGAVDPAPEGVGFHYIDHLTHNVMR
                     GNMDTWYRFYAETFNFREIRFFNIEGKVTGLHSRALTSPCGKIRIPINESADDKSQIE
                     EYLKEYKGEGIQHIAVGARDIYAATDAIAARGLEFMPGPPEIYYERSRERVVGHEEPI
                     ERMRRHGILIDGEGVVAGGTTRILLQIFSKTVIGPIFFEFIQRKGDDGFGEGNFRALF
                     ESIEEDQIRRGVLSAAK"
BASE COUNT         1060 a         2216 c         2235 g         1135 t
ORIGIN      
        1 gctcgggcac gtcgcgagcc ggccgccgat caatgtcgcc ttcgaggccg gacccaacgc
       61 cgcgaacgtc ttccagccgg cctgcacgct ctgcggcgac tgctgctcgg gttgcaatgt
      121 cggcgccaag acgacggtgc agatgagcta cctccccgac gccgtcaacc acggcgcgga
      181 ggtgttcacc gaggtcaccg cgcggcacgt gaaaaagctc ggcacgagcc acggccacac
      241 ctggcaagta ttctacgtgc cgacgcagga ccacagggag ggcttccgcc aggtcgagcg
      301 ggcgatctcc gcgcggatcg tcgtgctcgc ggccggcgcc ctcggctcga ccgaacttct
      361 gttgcgctcg cgcgaggagg gcctcgccgt gtcggaccgg ctcggcagcc gctnnnnnnn
      421 nnnatctctg gcgatccgct gcagacccag gcgcaagtgc tcgccttcgg ctacaacaat
      481 gatcgcgcgg tgaacgcagt cggcgtcggc catcccgctc ggcgcggcgt cagtccgccc
      541 ggtccctgca tcgccggcgc catcgacctc cgcgaccaga gccacctcga ggagggcatg
      601 atcatcgagg agggcgtgct gccgtccggc ctcgcgccgc tgctgccagg tatcttctcg
      661 gccggatttc acctgacggg gaaggatacc gaccagggcc tcgccgacgg cgccctcgag
      721 gccgcgcgac gggcgaagag ctgggtgctc ggcgcctact ggggcgccat gcagcacacc
      781 gagacattcc tcgtcatggc gcacgacgat gccggcgggc gtattgagct tgacgacggg
      841 cgcctcaccg tgcgctggcc gggcgtcgcc gagcagccga tctaccgcac gatcgaggca
      901 acactcacca aggcgaccgc ggcaaccggt ggcgccttcg ttcgcaatcc gctccagagt
      961 acgcagatcg tcggcaaaac actcgtcacg gtgcacccac tgggcggctg cccgatgggg
     1021 gcagatgcga gccgcggcgt cgtcgatcat gcctgccgcg tcttcgatcc ggccggcggc
     1081 gtgcacgagg gcctcctcgt ctgcgatggc gccgtgatgc cgcgcccgct cggcgtcaat
     1141 ccgctgttca cgatctcggc gatcgcggag cgcgccatga tcgagctcgc gcgccgtgag
     1201 aaccggccgc tgagcgacgc gccgagggcc gacgcgcggc tgcgtaagct tgtcggcgat
     1261 gaggacgacc gccggccggc cggcgtcgag ttcaccgagc gcatggcggg ctttctctcg
     1321 acagcgagcg ccgactatca gagcggcatg gcagcgggca agtccgccgg caccgagttc
     1381 gccttcacgg cgacgatccg cgtcgacgat atcgcccgct tcgaaagcga tcccgagcac
     1441 gctggccgca tctccgggac tgccctctgt ccgcagctct ccgctgatcc gctcgacatc
     1501 gccgacggcc gcttcaacct gatgcgcccg gaccctacgc gggtcgagac gcgccgcttc
     1561 gactaccaga tgcagctcat ctcgcgtgac ggccgccgct tccgcttcgc cggccacaag
     1621 gtggtgcgcg ccgatgcggg cgtcaccgac ctctggcgcg atacgacgac gctgttcgtc
     1681 gatctcgtcg aggagacggg gaccggcgcc accgcaaagg gaatcctgac gatcgccgcg
     1741 accgacttcg cgcgtcagct gaccacgctg aagggtaccg gcggcggctc gatcaaggag
     1801 cgcctcagcg cggtcggccg cttcggcggc gtcttcgccg gcacgctgtt cgacgtctat
     1861 ggcggccttg ccgcgccgct gaagcgctac gatggcacga tcgtccgcaa gaagcgggcg
     1921 ctgcgcacgc cgcacccgga agtgcatgaa ttcgcggcgc tcgacggacg caagctgcgt
     1981 ctcacgcgct accacggtgg cgacaagggc ccgctcctct tctcgcacgg actcggcgtc
     2041 tcgagcctca ttttctcgat cgacacgatc gatacgaacc tgctcgagtt catggtggcg
     2101 gccggctacg actgctggct cctcgacttc cgcgccagca ccgacttgcc acatgcacgc
     2161 gaccgctgga cggcggacga ttgcgcgacg aaggactatc agccggccgt cgatctcatc
     2221 cgcgagaagt ccggggcgac gagcgtgcag gtgatcgcgc attgcttcgg cgcgaccacg
     2281 ttcgtgatgg cgatgctttc cggcctgaag ggtgtgcgct cggccgtgat ctcgcagatc
     2341 gcgaccgacg tgatcgtgcc gttctggccg cagcgcgcgc tcgcctatct gcgcacgccg
     2401 tcactcttca gtctattggg catcggccat gtcaacgcgc gtgcgacgaa ggaagcgggc
     2461 gtcgccgagc ggctcatgga cggcctcatc cgtatcgccg tgcccttcca gcgcgacgag
     2521 cgcacgcgca acgccacctc gaaccggatc accgcgctct acggtcagct ctacgagacg
     2581 gcgcaactcg atgccgacac cttcgagttc ggcctcgccg agatgttcgg gccggcgaac
     2641 atcgacgcct tcaagcagct cgcgctcatc gcgcggcgac aggtgatcgt gaacgcggcc
     2701 ggcgaggacg tgtacctccc gcatctcgag cgcctggcga tcccgatcac aatcatccac
     2761 ggcgcaaata acgcctgctt ccgccccgag agcacggtgc gcacgttgga gcggctgacg
     2821 aaacgcaacg gcgcgagctt ctatcagcgc cacgagattg ctggctacgg ccacatcgac
     2881 tgcatcttcg gcaagcgcgc cgccgtcgat gtctacccga tcatgctcgc ccacttcgag
     2941 ccgacggcgc tgcaccgggt gtgagggtgt gggctgtggt cgttgggtcg cctcaccccc
     3001 actcccgctc ctttggcgcc gggagcacct tgccgcggca ctcgccgaag ccgatgcgga
     3061 cgccgtcctt ctcgcagtgt ccggtgagga cgagctcgtc accgtcctcg aggaaggtgc
     3121 gttttgcgcc gccttcaagg ccgagcggct ccttgccgcc ccaggtgatc tcgacgagcg
     3181 agccgaggct gtctttagtc ggccccgaga tggtgcccga gccgagtagg tcgcccgtgc
     3241 acatctcgca gccgccgact gcgtgatggg cgagctgctg ggcggccgag tagtacatgc
     3301 ccttgtagtt ggttcggctg atggtggtcg gtcggttcga tcccgcaggc gtcagcgcaa
     3361 cctcgagctt caggtcgaag ttgttcgccc cgcgctcgac gaggtaggga agcagcggct
     3421 tcacgcgctc gggcgttgcg acgcggaagg gctccagcgc ctccgccgtc accacccagg
     3481 ggctgatcga ggtcgcgaac gctttcgact ggaacggccc gagcggctgg tattcccagg
     3541 cctggatgtc gcgcgccgac caatcgttga gcagcacgta gccgaagatc atgtcgtagg
     3601 cttcggcgac ggtcaccggc tcgcccatgc ggtttcccgt gccgacgatg gcgccgagct
     3661 cgagctcgaa gtcgagccgc ttgcaggggc cgaaggacgg catcgccgca tccggcgcct
     3721 tggtctggcc gagcggacgg cggatgtctg tgccactcac gacgacggtc gaggcgcggc
     3781 cgttgtagcc gatcgggatg tgcagccagt tcggcatgag ggcgttctct ggcccgcgga
     3841 acatggcgcc gacgttggtt gcatgctcct tcgaggcgta gaagtcggtg aagcttctga
     3901 cctcgatcgg caagtgcaag gacgccgccg acatcggcac gaggattttc tcatgcagcg
     3961 cacggtcgat cgttttgtcc gccggattgc gggcatcgag aaagcgcgag atgtggccgc
     4021 gcatctcccg ccagatcgtg cggccggtcg ccatgaaggc attgagcgac ggctgcggaa
     4081 atatctccgc gccgatgtgg ccggcgcgca gatgacccgc ctcgaaggtg gcggagagat
     4141 cgaggatttg gtcgccgatg ccgaccccca tccgccgtgt gcggcccggc gcactgaaga
     4201 tgcagaaagg caggttctgg atcgggaagg ggcagccggg aacattggcc gatgcgaccc
     4261 agctcttcaa ggcggggtca tgcgtctcgt tgagtgccat ggatcggctc cttcgatcgg
     4321 tgatcagcgc ttcttcggat cgaagtgctt cttgatgccg tgccagcagt cgacatagtc
     4381 gccctgcagc cccgcctgct cggcagcgta gcgcgtcacg cgttgcggga agcgcgtctc
     4441 gaacatgaaa gccaacgtat tctgctgctt gtgcggctcg agcttcacgt tgctcgcctt
     4501 ctcgaaggcc tcggtgtccg gcccgtgcgg cagcatggag ttgtgcagcg acatgccgcc
     4561 cggcacgaag ccgtggggct tcgcatcata ggcgccatag acgaggccca tgaactcgct
     4621 catcacgttc aggtgatacc agggcgggcg gaaagtgttc tccgcgacca tccagcggtc
     4681 cgagaacacg acgaagtcga tgttcgccgt gccgggcgtt tccgacggcg aggtcatcac
     4741 tgtgaagatc gacgggtcgg cgtggtcgaa aaggatcggc cccacgggcg agtagtggcg
     4801 gaggtcgtat ttgtagggcg cgaagttgcc gtgccacgcg acaacgtcga gcggcgaatg
     4861 cccgatctcg gctgcccaga gcgtgccgcc ccatttgaca aacagccggc agggctggtc
     4921 gcgatcctcg taggccgcga cgggagtgag gaagtcgcgc tggttcgcca tacagttggc
     4981 gccgatcggc ccgcgctccg gcagcgtgaa ggccgcgccg tagttctcgc agagatagcc
     5041 gcgcgcggcg ggatcgccat tcagctcgac cgcgatcttc acgccgcgcg gaatgacggc
     5101 gatctccgcc ggctcgatgt cgatcacgcc gaactcggtg cggatgcgca gccgcccctg
     5161 ctgcggcacg aacatcatct cgccgtcggc gttgtagaag tattcgtcct tcatcgagcg
     5221 cgtgacgagg tagacgtttg cgcccatgcc gccgagcgtg ccggcatcgc cgcacgtggt
     5281 catggtgcgg atgccctcga tgaacgatac gctgcctttc ggcagtgccg gcgggtccca
     5341 ccgcattggc gctggcggta cctcgacctc ggtgcatggc gccgtccgcc agaggccggc
     5401 gtcggccttc ttgaactgtc cccagtgctt caccgtcggg cggatgcgat agagccagct
     5461 ccgctcgttg gagacgcgcg gcgccgtaaa gggcgagccc gagatctgct cggcgtagag
     5521 gccgtaaggg cacttctgcg ggctgttgcg gccgacgggc agcgcgcccg gcaacgcctc
     5581 gctctcgaag ccgttgccga agcccgacat gtagccgcga ttgccctccg cgatagagga
     5641 cgcgctgtcg cgagcggggc gcatgacggt gatgtcattc atcgggtcgt ctcctcgaag
     5701 cgaaggcggc taagcgagcg ttcggagcaa ctgtgcgcca ctcgcgctcg tcactcaagt
     5761 atgcgtgcat ggcctcactt tgcggcgctg agcacgccgc ggcggatctg gtcctcctcg
     5821 atcgattcga agagcgccct gaagttgccc tcaccgaagc cgtcgtcacc cttacgctgg
     5881 atgaactcga agaagatcgg gccgatcacg gtcttcgaga agatttggag gaggatgcgc
     5941 gtcgtgccgc ccgcaaccac gccctcgccg tcgatcagga tgccgtggcg gcgcatgcgc
     6001 tcgatcggct cctcatgccc gacgacgcgc tcgcgcgagc gctcatagta gatctccggc
     6061 ggacctggca tgaactcgag gccgcgcgct gcgatggcgt ccgttgccgc gtagatgtcg
     6121 cgcgcaccga cggcgatgtg ctggatgccc tcgcccttgt actccttgag gtactcctcg
     6181 atctggctct tgtcgtccgc gctctcgttg atcgggatgc ggatcttgcc gcaggggctc
     6241 gtcaatgcgc ggctgtgcag ccccgtcacc ttgccctcga tattgaagaa gcggatttcg
     6301 cgaaaattga aggtctcggc gtagaatcga taccaggtgt ccatgttgcc gcgcatgacg
     6361 ttgtgcgtca ggtggtcgat gtaatggaag ccgacgccct cgggcgccgg atcgaccgcg
     6421 ccgagccagt cgtagtcgcg ggcatagggt gagcctttgg caccgtaggt ttcgacgaga
     6481 tagaggagcg agccgccgat gccgacgagc gccggggcgt cgatcgactt gtcgctgccg
     6541 gtgtattcgc tcgcgccaag agaaagcgcg cgggcaagcg cgtggtgggc gtcgatcacg
     6601 cgccaggcca tggccggcgc gcaggggccg tgctcggcga cgaagcgcgc ggcatg
//
LOCUS       DAORNH010000259         6653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8541_length_6653_cov_2.909973, whole genome shotgun sequence.
ACCESSION   DAORNH010000259 DAORNH010000000
VERSION     DAORNH010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_6653_cov_2.909973"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..2084)
                     /locus_tag="PK264_20085"
     CDS             complement(108..2084)
                     /locus_tag="PK264_20085"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD78206.1"
                     /translation="MAIVVLDPGHGGTSMVGGSSANNAKGPKGTLEKHLTLKVAKAAQ
                     SALEAQGVDVRLTRTTDVNLGLAARAAIAKAAAAPVFLSIHFNGFNNDAQGTETLVAP
                     DASDRSRKLAKHVQSRVRAVTGLKNRGVKDQSLGVLRPASHHPKTAAALLEVSFMDVA
                     AEEERLQDRGYITAIGTAIADAIVAYLVEASLMAAPEVDAEVAMLEDGFMAARRGSRA
                     AALELQAADDAAPVGMAIDAEIPFIEHGDQSRSFGDIGRDSSVDDPEHMTRGWRSYRN
                     DIELTPDFEAVIGQDKSLPATFLQVMADRRRAVGKISASGVNYRGQRGSWSGTGFVVG
                     KNLLLTNHHVLNSADVAQGAVIDFEFELPTADFLAGVAEPSDQPAANRRRFKLDPARL
                     FVTSPATEGGLDYTFVWVDEAAVAAAGPIPMHRANFAVNTNEQAFVVHHPEGRAKRVS
                     LDDTDVLIINTAVIRYTSDTMPGSSGSPVFNRQGRLIALHHASRRGPVQRDDGRTLDV
                     LNEGVKISAIVLDLERRHTGEERAMIGEVLAAVHGSDTMSGFFGALGREREARAADTS
                     VEAVVDAYRGTEADIDIGFWNIEWLSKRYTDRGKLRMAAALIVDLGLDIWGLSEVSPS
                     AVKALVGKESALMAARHRANIPDIRHWCDHVEGH"
     gene            complement(2282..3163)
                     /locus_tag="PK264_20090"
     CDS             complement(2282..3163)
                     /locus_tag="PK264_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HRD78207.1"
                     /translation="MRAVLTLILGVLGAAAAALYSSAFIVGQNEQSLVLRFGEVRQDI
                     REPGLNWKMPLIDIVHTLDKRVLDLDSKPQIVSSSDPQFLSVDSFTRYRIIDPKLFFQ
                     RVRTEAEAGRRLSPIVDSTVRDVLANAKMVDIIRNKREHLMKEIARLVHDQAKDLGIE
                     IVDVRIKRADLPDANSQRIYERMRAERKQEADKIRALGAASAVETKAEADRKATVLRA
                     EARRKSDETKGIGEGERNRIYREAFDKDPEFFRFYRRMQACEQAFRAADTKFVITPGS
                     DICPEVSEGPAPAPARK"
     gene            complement(3165..4262)
                     /gene="hflK"
                     /locus_tag="PK264_20095"
     CDS             complement(3165..4262)
                     /gene="hflK"
                     /locus_tag="PK264_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HRD78208.1"
                     /translation="MPWSNQSGNGNGWKGSGGGGGPWGGGSGGGQQPDLEEFLRRGQD
                     RLKKAIPGGGPPGLLVLLIGLVAAVFVGFFAFTFRVDQDEVGVVTRFGKYDREAPPGL
                     HFRLPAPIEEVRTPKVTRQNLTEVGFRSGGTGRVASSGANRDVPEESVMLTGDQNIVD
                     VDFVIYWRIKDAKLYLFNIQNPDGTVKEVAESAMREVIGKADIQPILTEARQATSKAV
                     HELMQTILDNYKAGITIDEVRLLKVAPPAQVADAFLDVQAARADRDRLQNEAEAYKNR
                     IIPEAEGEAKRIEKEADAFREQTIAEAIGQTKRFLSVYEEYRKSPDVTRRRIYLETME
                     RILSGSDKIILDSRGGPSPILPLDMFRRKGN"
     gene            4699..5109
                     /locus_tag="PK264_20100"
     CDS             4699..5109
                     /locus_tag="PK264_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD78209.1"
                     /translation="MDVHVGSRVRLRRMLLGISQEKLGEKLGLTFQQIQKYEKGVNRI
                     GASRLYDLASVLSVPVQFFYDDAPVAESLAQPVMGFADRPADSYVVDFLATREGLELN
                     KAFVKITDPRQRRAVLELVRAMASEAAEDTVGAD"
     gene            5238..6563
                     /gene="metK"
                     /locus_tag="PK264_20105"
     CDS             5238..6563
                     /gene="metK"
                     /locus_tag="PK264_20105"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRD78210.1"
                     /translation="MRVNTIRWGPPVARTSFLFTSESVSEGHPDKVADQISDAILDAF
                     IANDVKLGIADNSAINTRLGCETLVTTNKVVVAGEARGQAPLFRKFASHQFVGQNEWV
                     VDRGLITDIARAVIKDIGYDQVGFSHHGADVEVLLHGQSADIAMGVDEKQGEEGAGDQ
                     GMMFGFACTDSEAYEKGTFMPAPIFFSHKILKVLSEQRRSGKLGDLLPDAKSQVTIQY
                     EGGKAIGATKVVVSTQHQKSNGKGKDYTPEMIKEMIGDAVTSALPKGWMPKSGADFLV
                     NPTGNFVVGGPDGDCGLTGRKIIVDTYGGYAPHGGGAFSGKDPTKVDRSAAYAARYLA
                     KNVVAAGLADQCMIQVAYAIGRPEPMSLLVDTKGTGKHGADGDAKLEKILAELFPMRP
                     THIRRALALNKPVYKRTAAYGHFGRAPDKDGGFSWEKTDLADKIKKAMG"
BASE COUNT         1128 a         2115 c         2187 g         1223 t
ORIGIN      
        1 tctcgcttcc attaattcac tatttgtatt tcccgtcgta tgaattggcc gcgtgtctgg
       61 ctcgattgcc ttccctcgat cggacgactg ttgttctgga cgggatgtca gtggccttcg
      121 acgtggtcgc accagtggcg aatatcggga atattggccc tgtgccgggc cgccatcagg
      181 gcggattcct tgccgacgag cgccttcacc gcgctcggcg aaacctcgga gagcccccag
      241 atgtcgaggc cgagatcgac gatgagggcg gcggccatgc ggagcttgcc acggtccgtg
      301 tagcgcttgg acagccactc gatgttccag aagccgatat cgatgtcagc ctcggtgccg
      361 cggtatgcgt cgaccacggc ctcgacgctg gtgtcggccg cgcgggcctc acgctcgcgc
      421 ccgagcgcgc cgaagaagcc cgacatcgta tcggagccgt gcaccgctgc gagcacctcg
      481 ccgatcatgg cgcgctcctc gcccgtgtgc cgccgctcga gatcgaggac gatcgccgag
      541 atcttgacgc cttcgttgag cacgtcgagc gtgcgcccgt cgtcgcgctg cacagggccg
      601 cgccgactcg cgtgatggag cgcgatgagg cggccctgcc gattgaacac gggcgagccc
      661 gaggagcccg gcatcgtgtc cgaggtgtag cggatgacgg cggtattgat gatcagcacg
      721 tcggtgtcgt cgagcgagac gcgcttggcg cgcccttccg gatgatggac gacgaacgcc
      781 tgctcgttgg tgttcacggc gaagttggcg cgatgcatcg ggatcggccc ggccgcagcc
      841 acggcggcct cgtcgaccca cacgaacgtg tagtcgaggc cgccctctgt cgccgggctc
      901 gtcacgaaga gccgggccgg atcaagcttg aagcggcggc ggttcgcggc cggctggtcg
      961 ctcggctcag caacgccggc aaggaagtcg gccgtcggca gctcgaactc gaaatcgatg
     1021 acggcgccct gcgccacgtc tgccgaattg agcacgtgat ggttggtcag cagcaggttc
     1081 ttgccgacca caaagccggt gcctgaccag gagccgcgct gtccccggta gttgacgccc
     1141 gaggcgctga tctttccgac cgcgcgccgc cggtcggcca tcacctggag gaaggtcgcc
     1201 ggcagcgatt tgtcctggcc gatgacggcc tcgaaatctg gggtcagctc gatgtcattg
     1261 cggtagctgc gccagccgcg cgtcatatgt tcgggatcgt cgacagagct gtcgcgaccg
     1321 atgtcgccga acgagcgcga ctggtcgccg tgctcgatga aggggatctc cgcgtcgatg
     1381 gccatgccaa ccggtgccgc atcatccgcg gcctggagct cgagcgcggc cgcgcgactg
     1441 ccgcgtcttg ccgccatgaa gccgtcctcg agcatcgcca cttcggcatc gacctcgggc
     1501 gccgccatga gcgaggcctc gacgaggtag gcgacgatgg cgtcggcgat cgccgtgccg
     1561 atcgctgtga tgtagcctcg atcctggagc cgttcctcct cggcggcgac gtccatgaaa
     1621 ctcacttcga ggagcgcagc cgctgtcttc ggatggtggc tcgcgggccg cagtacgccg
     1681 agcgattgat ccttgacgcc gcggttcttt agcccggtga cggcgcggac gcggctctgg
     1741 acgtgtttcg ccagcttgcg cgagcgatcc gaggcatcag gcgcgacgag cgtctcggta
     1801 ccctgtgcgt cgttattgaa gccattgaag tggatcgaca ggaacacggg cgccgcagcg
     1861 gcctttgcga tcgcggcgcg ggcggcgagg ccgagattca cgtcggtcgt ccgcgtcaag
     1921 cgcacgtcga cgccttgcgc ttccagcgct gattgggcgg ccttggcaac tttcagcgtc
     1981 agatgctttt cgagcgtgcc tttcggacct ttggcattgt tcgcggagga gccgccaacc
     2041 atgctcgtgc cgccgtgacc gggatcgagt accactatcg ccatgaccga actccgtgtt
     2101 gaaaaagagc aagcaacacg gtcatggttt cacaagcaat ggggcgccgt cgtcatccgt
     2161 tcggtggaaa atcgcggcgc tcaggggcgc ggcgctcagg caagcaagac atgctctaga
     2221 cggcagcggg gtgcgcatct gccgatgcgc accccgctcg acgtctcgtt ttcgtcgcgc
     2281 gctacttccg agcaggagcc ggcgccggcc cctcgcttac ctcggggcag atgtcggagc
     2341 cgggcgtgat cacgaacttc gtgtcagccg cccggaaggc ctgctcgcac gcctgcatgc
     2401 gccggtagaa gcggaagaac tccgggtcct tgtcgaacgc ctcgcgatag atgcggttgc
     2461 gttcgccttc gccgatgccc ttggtttcgt cggattttcg ccgcgcctcc gcgcgcagca
     2521 ccgtcgcttt ccggtcggcc tcggcctttg tctcgacggc gctcgctgca ccgagcgcgc
     2581 ggatcttgtc ggcttcctgt ttgcgctccg cgcgcatgcg ctcgtagatg cgctgcgaat
     2641 ttgcgtcggg cagatcggcg cgcttgatgc ggacatcgac gatctcgatg ccgaggtcct
     2701 tcgcctgatc gtgcacgagg cgggcaatct ccttcatgag atgctcgcgc ttgttgcgga
     2761 tgatgtcgac catcttggcg ttggccagca cgtcgcgcac tgtcgaatcg acaattggcg
     2821 acagccgccg cccggcctcg gcctccgtcc gcacgcgctg gaagaacagc ttcggatcga
     2881 tgatcctgta gcgcgtgaag ctgtcgaccg agaggaactg cggatcggac gacgagacga
     2941 tctggggctt cgagtcgaga tcgaggaccc gcttatcgag cgtgtgcacg atgtcgatca
     3001 gcggcatctt ccagttgagc cccggctcgc ggatgtcctg gcggacttcg ccgaagcgga
     3061 gcacgagcga ctgctcgttc tggccgacga tgaaggccga gctgtataag gctgcggccg
     3121 cggcgccgag cacgccgagt atcagtgtca ggacggcgcg catgtcagtt ccccttccgg
     3181 cgaaacatgt cgagcggcag gatcggcgat gggccgccgc gggaatccag gatgattttg
     3241 tcggagccgg agaggatccg ttccatggtc tcgaggtaga ttcgccgccg cgtcacgtcc
     3301 ggtgacttgc gatactcctc gtagacgctg aggaaccgtt tcgtctggcc gatggcttcg
     3361 gcgatagtct gctcccggaa ggcgtcggcc tccttctcga tccgtttcgc ctcgccttca
     3421 gcctccggaa tgatgcggtt cttgtaggct tcggcctcgt tctgcaagcg gtcacggtcg
     3481 gcgcgggcgg cctgcacgtc gaggaaggcg tcggcgacct gggccggagg cgcgaccttc
     3541 aggaggcgca cctcgtcgat cgtgatgccg gccttgtagt tgtcgaggat agtctgcatg
     3601 agctcgtgca ccgccttcga ggtggcctga cgggcctcgg tcaggatcgg ctggatgtcg
     3661 gccttgccga tgacctcgcg catggcgctc tcggcaacct ctttcacggt gccatccgga
     3721 ttctggatgt tgaagaggta gagtttggcg tccttgatgc gccagtagat cacgaagtcg
     3781 acgtcgacga tgttctggtc gcccgtcagc atgacgcttt cctcgggcac gtcgcggttc
     3841 gcaccacttg aggcaacacg cccggtaccg ccggaccgga atccaacctc ggtcaagttc
     3901 tggcgcgtca cctttggtgt gcgcacctcc tcgatcgggg cgggcagacg gaaatggagc
     3961 cctgggggcg cttcgcggtc atacttgccg aaacgcgtga cgacgccgac ctcgtcctga
     4021 tcgacgcgaa aagtgaacgc gaaaaacccg acaaatacgg ccgcgaccag gccgatcaga
     4081 agcaccagca ggcccggcgg gccgcctcca gggattgcct ttttcaaccg atcctgtccg
     4141 cgcctcagga actcctcgag atcgggctgc tgtccgcctc cgctgccacc gccccaaggg
     4201 ccaccgccgc cgccgctccc tttccaaccg ttcccgttac cgctctggtt actccagggc
     4261 atatggccgc ctctcgctga accgaatggg tcgatggccc caagggcgtt tgccgggttc
     4321 ctgcggctgc aggagcgtcg gcgcacagcc ccggccaagt cctggcgctt atacggtagg
     4381 gggtcatgtg tggcaaatac gtggatatgc ggacgtcatc gattttgccg gatcggcaac
     4441 taccggatgc gatttgccca ggcgtcgtcg cggccctgcc caccatcccg gtcggtggtg
     4501 gcaatcggct ccggcaatcg ataaactcga ggttgacgag aattggcgct gcacttaaaa
     4561 agtgcaagtg ggggacatcg gatatgatgt tcttggtttg caagacgtgg gtggtgcggg
     4621 atggtgtcgc caagcgtagt ggacgggtcg gcgggggacg tcgcaggcga ggatcgcagc
     4681 tcgcgccgtg cgaacccgat ggatgtgcat gtgggtagcc gtgtccgcct ccggcgcatg
     4741 ctgctcggca tcagtcagga gaagctcggg gagaagctcg gactgacgtt tcagcagatt
     4801 cagaagtatg agaagggcgt caatcgcatc ggcgcgagcc gcctctatga ccttgcgagc
     4861 gtgctgagcg tgcccgtgca gttcttctat gacgatgcgc cagtcgcaga gtctttggct
     4921 cagcccgtga tgggctttgc cgatcgcccg gcagactcct acgtcgtcga ctttctcgcg
     4981 acccgcgaag gcctcgagct caacaaggcg ttcgtgaaga tcaccgaccc acggcagcgt
     5041 cgcgcggtgc tggagctcgt tcgagccatg gcaagtgagg ccgccgaaga caccgtcggc
     5101 gcggactgac gaataggggc cgcgaggcgc tgccggcggt gatcgtcagg tatccacatg
     5161 ctgcggactt gcggggcccc gggccgcgcg catctgcttg accctgaccg gaaaggctgc
     5221 aagagaagcc gcgcctgatg cgagttaaca ctatccgatg gggaccacct gtggcgcgaa
     5281 cgtctttcct gttcaccagc gaatctgttt cggaaggtca tccggacaag gtcgcggatc
     5341 agatttcgga cgccatcctc gacgccttca tcgccaatga cgtgaagctc gggatcgccg
     5401 acaacagcgc catcaacacg cgtctcggct gcgagacgct ggtcaccacg aacaaggtcg
     5461 ttgtcgccgg cgaggcacgt ggccaggcgc cgttgttccg caagttcgca agccaccagt
     5521 tcgtcggcca gaacgagtgg gtcgtcgacc gcggcctcat cactgacatc gcgcgcgccg
     5581 tcatcaagga catcggctac gaccaggtcg gcttctcgca ccacggcgcc gatgtcgagg
     5641 ttctcctgca cggccagtcg gccgacatcg ccatgggcgt cgatgagaag cagggtgagg
     5701 aaggcgccgg cgaccagggc atgatgttcg gcttcgcttg cacggacagc gaggcgtacg
     5761 agaagggcac gttcatgccc gcgccgatct tcttctccca caagatcctg aaggtgctct
     5821 cagagcagcg ccgttccgga aagctcggtg atctcctccc cgacgccaag agccaggtga
     5881 cgatccagta cgagggcggc aaggcgatcg gtgccaccaa ggtcgtggtc tccacgcagc
     5941 accagaagtc caacggcaag ggcaaggact acacgccgga aatgatcaag gagatgatcg
     6001 gcgatgccgt cacctctgca ttgccgaagg ggtggatgcc gaagtcgggc gccgacttcc
     6061 tggtgaaccc gacaggcaac ttcgtcgtcg gcggcccgga cggcgactgc gggttgaccg
     6121 gccgcaagat catcgtcgac acctacggcg gctatgcgcc ccacggcggc ggtgccttct
     6181 ccggcaagga cccgaccaag gtcgaccgct ccgctgccta cgccgcacgc tatctcgcta
     6241 agaacgtggt tgccgccggt ctcgccgacc agtgcatgat ccaggtcgcc tatgcaatcg
     6301 gccggcctga accgatgtcg ctcctcgtcg ataccaaggg cacgggcaag cacggcgccg
     6361 atggcgacgc caagctcgag aaaatcctag ctgagctctt tccgatgcgc ccgacccaca
     6421 tccggcgtgc gcttgcgctc aacaagcctg tctataagcg cacagcggcc tacggccact
     6481 tcggccgcgc acccgacaag gatggcggct tctcctggga gaagaccgac cttgccgaca
     6541 aaatcaagaa ggcgatgggc tgatcaggga gagtaggctc gaggggccgc tccccgcgcc
     6601 cgcggcatag ggatgctccc tacccccgaa gatgcgacga acaggacggc acg
//
LOCUS       DAORNH010000260         6652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8543_length_6652_cov_2.192663, whole genome shotgun sequence.
ACCESSION   DAORNH010000260 DAORNH010000000
VERSION     DAORNH010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_6652_cov_2.192663"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>340
                     /locus_tag="PK264_20110"
     CDS             <1..>340
                     /locus_tag="PK264_20110"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRD78211.1"
                     /translation="VTTGVCEAEARHVTLGHIRRVTSRRPFVQLKVAVDADGFVPRGA
                     AGRPLWISSPDARRRSHLLRAEADAILVGRGTVVADDPELTCRLPGLENRSPHRVVLA
                     SHLDISAGCKL"
     assembly_gap    341..403
                     /estimated_length=63
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <404..745
                     /locus_tag="PK264_20115"
     CDS             <404..745
                     /locus_tag="PK264_20115"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRD78212.1"
                     /translation="AERESALVARGATVVRTAATSTGRLDLAAVLQYLSETGLTRVLV
                     EGGTTVLGGFLDRLADELVVIQSPDRLPPAVRLRPFGDRMIGDLARLGWRVAGSELVG
                     PDTLTTYRPEG"
     assembly_gap    767..791
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <792..1200
                     /locus_tag="PK264_20120"
     CDS             <792..1200
                     /locus_tag="PK264_20120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRD78213.1"
                     /translation="CDSQHVVFNARYGDYVDLAITEFLRASMPGRDPFDGSFEIQTVR
                     QLIEWQSPFRFRDTVEVTVRPTRFGTTSFTIGFEMRRAGEPEIVASAELVYVHVDSKT
                     WTKLPLPAERRRMLETGALGKVTDHAGWTIGRS"
     gene            1307..1897
                     /locus_tag="PK264_20125"
     CDS             1307..1897
                     /locus_tag="PK264_20125"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRD78214.1"
                     /translation="MHPKLERLDASRIKIRARADLDITLFANDGVFLDPASVEEMVSF
                     AGLVDTIEALNARRFFGDEPAHLERVVLTPDFHRAAGIPVGTVLDARGFVLPKAVGTD
                     IGCGMRLLATDITADEFAGLGPGLEERLRYIFFEGGRDIALSASQRRGIFGEGLQGLL
                     GASEQLKERRVRATGGSAGGRGKDTAATASSRSPTR"
     gene            1894..2790
                     /locus_tag="PK264_20130"
     CDS             1894..2790
                     /locus_tag="PK264_20130"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRD78215.1"
                     /translation="MTGPVEGPRTHGSGAEFTRDGQIGSIGGGNHFVEIQRVEEVFDG
                     ATARAWGLVPGRVAIMAHSGSVGIGHMVGNHFSDKARALYPAGLAHPEHGFYPLGSRS
                     DAGEAYLSAMGNAANFAYANRLFLGLMVVRALSEALGREVEARLVYDAPHNLVWDDGD
                     GRMIHRKGACPADGPSDDADFPSGHPVIIPGSMGASSFVLRGHGSVASLCSACHGAGR
                     LAPRQQARHGAEGELAALRVVTKVDGRRLRPDVRAEWQRALSEEAPSRYKDVTPVIET
                     VVGADIASKVVRLWPLMTVKGL"
     gene            complement(2802..3800)
                     /locus_tag="PK264_20135"
     CDS             complement(2802..3800)
                     /locus_tag="PK264_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78216.1"
                     /translation="MASDGLICDVLSEIVALIAAARSTPLAKLKLERVVARIETLPLD
                     ALDPISVALRDFPIQPPQALIRFTTEESWRAIAPEPLAPLLLFHRSGFVRQAALNRTS
                     SLPDGPILVVALAQRLNDWVEPVRAAAAACAERVLPSVSAKTMAAAAPYLLARFHSWQ
                     RWGEPPPVLLEALRRDDRAAALAATLIATTSIPGSVLKSAIRLGIVDRHLAEIARRAR
                     RPEFRAAALRPLIDGRVSWLDHYDKTWVDKRFGIARRTAVLAHRPIERQIALSELIGI
                     GAKDASALVRYVAAERLVCHAGEIGDTTALTALFKGDKSRGVRWLMAFLARQKAGG"
     gene            complement(3782..4660)
                     /locus_tag="PK264_20140"
     CDS             complement(3782..4660)
                     /locus_tag="PK264_20140"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HRD78217.1"
                     /translation="MEGVSATRRLVVCADDYAFTPGVSRAIRELLARRAISATSVMAA
                     SRFWPEEAAALREVAGDADIGLHVTLTDQTPLGAMPTYAPDGKLPPIGAVFKAGLLRR
                     LPLGEIEAEIERQLASFIDVWGRPPAHIDGHHHVQQLPGVRDIVVRLAKRVGGGETWV
                     RSGAEPASRVLARGVATAKSLLISSLGRGIRRTAARAGVPTNDGFSGAYDYQTDKRPM
                     AELFQAFIKGAGENALLFCHPGYNDATLEGLDGMTTIRDAEQAFLLGDDWPDMVAKSG
                     LEIGPYRRNPAWRATG"
     gene            complement(4648..5439)
                     /locus_tag="PK264_20145"
     CDS             complement(4648..5439)
                     /locus_tag="PK264_20145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78218.1"
                     /translation="MAGKGVAVITGGASGIGEATARRFARDGFDVAILDMNEGAGTKL
                     AVELSKSGVKARFWRCDVSDLKAVEAVAAQTAKDLGPATALVTCAGLIPNSESILEMD
                     LVAHERMWAVNYNGTLYAARVYGKQMIPAKGGTIVTIGSINSRLPLPLAAYNPGKAAV
                     ERLTQLLAVELGRFGIRVNSVGPTYVMTPPLREKVASGQRDMKKIMGVHALDRLPEPS
                     DIADAIAFLSSDQARSITGILLPVDAGWLSAVSYKTYAGGVPWKE"
     gene            5606..>5982
                     /locus_tag="PK264_20150"
     CDS             5606..>5982
                     /locus_tag="PK264_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78219.1"
                     /translation="MTLILLAKLTVTPALVWLAAYVMRRYGGLLAGMLVGFPVMTAPV
                     AFFMAWEQGREFAEESALGILLTVPAVSMFAIAYGLASRRGGWRAGWAAGVAAFFAVS
                     VATVTLRLDIPPAAAIAALVIAIG"
     assembly_gap    5983..5992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5993..6403
                     /locus_tag="PK264_20155"
     CDS             <5993..6403
                     /locus_tag="PK264_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78220.1"
                     /translation="LYLVGRPPVKAPARAAPWWDVWLRMAITMALVIAVTAAANAMGP
                     RWSSIPASYPAITSVVIPFTHARDGRDAALAVVRGMLLSHIAFAALFVIAAALIADHG
                     VIVAYAVATGAALGLSGIVISCDRWLARRRPTTV"
     gene            complement(6410..>6652)
                     /locus_tag="PK264_20160"
     CDS             complement(6410..>6652)
                     /locus_tag="PK264_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78221.1"
                     /translation="YALTELGRSLSDAVRKLDLLDQNSMQLPLRMRPGSHAAAILWSQ
                     QFTGDAVGLDKLLAAQLLEGVPGGTGIPARAVKTGR"
BASE COUNT          944 a         2253 c         2282 g         1075 t
ORIGIN      
        1 ggtgacgacg ggcgtctgcg aggccgaggc gcggcatgtc acactcggcc atatccggcg
       61 cgtcacctcg cggcgcccgt tcgtgcagct gaaggtcgcc gtggacgccg acggcttcgt
      121 cccgcgcggc gccgcgggtc gcccgctctg gatttcctcg cccgatgcgc gccgccgttc
      181 gcatctcctg cgggcagagg ccgacgcgat cctcgtgggt cgcggcaccg tcgttgccga
      241 cgatcccgag ctcacctgcc gccttccggg cctcgagaat cgctcgccgc atcgtgtggt
      301 gcttgcttcg catctcgaca tttcagcagg gtgcaagctg nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccgagc gcgagtcggc
      421 actggttgcg agaggcgcga ccgtcgtgcg caccgccgcg acctcgacgg ggcggctcga
      481 tctcgccgcc gtactgcaat atctctccga gaccggcctt acgcgcgtgc tggtcgaagg
      541 cggcacgacc gtgctcggcg gcttcctcga ccggctcgct gacgagctcg tcgtgatcca
      601 gtcaccggac cgcctgcctc cggccgtccg cctgcgcccg ttcggcgacc gcatgatcgg
      661 cgacctcgcc cggctcggct ggcgcgtcgc cggcagcgag ctggtcggac ctgatactct
      721 cacaacctac cgacccgagg ggtgattcat gagcaagccc actccgnnnn nnnnnnnnnn
      781 nnnnnnnnnn nctgcgacag ccaacacgtg gtcttcaacg cgcgctatgg cgactacgtc
      841 gatctcgcca tcaccgagtt cctgcgcgcc tcgatgccgg gtcgcgatcc ctttgacggg
      901 tccttcgaga tccagaccgt gcgccagctg atcgaatggc agagcccgtt ccgcttccgt
      961 gacacggtcg aagtgacggt gcgtccgacg cgcttcggca ccacgtcctt cacgatcgga
     1021 ttcgagatgc gtcgcgcggg cgagcccgag atcgtcgcct ccgccgagct cgtctacgtg
     1081 cacgtggaca gcaagacctg gaccaagctg ccgctccccg ccgaacgtcg gcgcatgctc
     1141 gagacgggcg cgctcggcaa ggtcacggat cacgccggct ggacgatcgg gcgtagctga
     1201 gtgcgccttg gtgcgcttga ccgccgggaa attcgctcta tctgtcgcat gagcttttgt
     1261 cgtgtgccgc ggctgctgaa cccgtcactc gacaaggaca cgtgccatgc accccaaact
     1321 ggagcgtctc gacgcgagcc ggattaagat ccgcgcgcgc gctgatctgg acatcaccct
     1381 cttcgccaac gacggcgtgt tcctcgatcc cgcaagcgtc gaggaaatgg tctcgttcgc
     1441 cggtctcgtc gacacgatcg aggcgctgaa cgcgcgtcgc ttcttcggcg acgagcccgc
     1501 acacctcgag cgcgtcgttc tcacgcctga tttccatcgc gccgccggca tcccggtcgg
     1561 caccgtgctc gacgcgcgcg gcttcgtgct gccgaaagcg gtcggcacgg acatcggctg
     1621 cggcatgcgg ctgctcgcga ccgacatcac cgccgacgag ttcgcgggcc tcgggccggg
     1681 gctcgaggag cggctgcgct acatcttctt cgagggcggg cgcgatatcg cgctctcggc
     1741 ctcgcagcgg cgcggcatct tcggcgaggg gctgcagggc ctgctcggcg cgtcggagca
     1801 gctcaaggag cggcgggtgc gggccacggg cgggtcggcg ggaggcaggg ggaaggacac
     1861 cgcggctacg gcatcaagcc gctcaccgac gaggtgacgg ggccggtcga ggggccgagg
     1921 acccacggct cgggggccga gttcacgcgc gatggccaga tcggctcgat cggcggtggc
     1981 aaccacttcg tcgagatcca gcgcgtcgag gaggtgttcg acggagcgac ggcgcgcgcc
     2041 tggggactgg tgcctgggcg tgtcgccatc atggcgcact cgggctcggt cggcatcggt
     2101 cacatggtcg ggaaccactt ctcggacaag gcgcgcgcgc tctatccggc aggcctcgcc
     2161 catcccgagc acggcttcta tccgttgggc tcccgcagcg acgcgggcga ggcctacctc
     2221 tcggccatgg gaaacgcggc gaatttcgcc tacgcgaacc gcctgttcct cgggctgatg
     2281 gtggtgcgcg cgctctccga ggcgctcggc cgcgaagtgg aagcgcggct cgtctacgac
     2341 gccccgcaca acctcgtgtg ggacgacggc gacggccgca tgatccaccg gaagggcgcc
     2401 tgccctgctg acgggccgtc ggacgacgcc gactttccct cgggccatcc ggtgatcatt
     2461 ccgggctcga tgggcgcgtc gagcttcgtg ctgcgcggtc acggctcggt cgcgagcctc
     2521 tgctccgcct gtcatggcgc ggggcggctc gcgccgcgtc agcaggcacg ccatggcgca
     2581 gagggcgagc ttgccgcact ccgcgtcgtc accaaggtcg atggccgccg ccttcgcccc
     2641 gacgtgcgcg cggagtggca gcgcgccctc tcggaggagg cgccgtcccg ctacaaggac
     2701 gtgactccgg tgatcgagac cgtggtcggc gccgacatcg cgagcaaggt cgtgaggctc
     2761 tggccgctga tgacggtgaa ggggctgtga taccggcaac ctcatccacc tgccttctgg
     2821 cgagcgagga atgccatcag ccagcggacg ccgcggctct tgtcgccctt gaagagggcg
     2881 gtcagagccg tggtgtcgcc gatctcgccg gcatggcaga cgagccgctc ggccgcgacg
     2941 taacgcacga gcgcacttgc atccttcgcg ccgatgccga tcagctcgga aagtgcgatc
     3001 tgccgctcga tcgggcgatg cgcaaggacg gcggtgcgtc gcgcgatgcc gaaccgcttg
     3061 tccacccacg tcttgtcgta gtggtcgagc cagctcacgc ggccgtcgat cagcggccgc
     3121 agcgccgcgg cgcgaaactc cggccgcctg gcgcggcgcg cgatctcggc gagatgccta
     3181 tcgacgatgc cgaggcggat agccgacttg agcacgctcc cgggaatgga cgtcgtcgcg
     3241 atcagcgtcg cggcgagtgc ggccgctcga tcatcgcgac ggagcgcctc gagcagcacg
     3301 ggcggcggct ctccccaacg ctgccacgag tggaagcgcg ccagcagata gggcgcggct
     3361 gccgccatgg ttttcgcgga gactgacggc agcacacgct ccgcacacgc ggcggcggcc
     3421 gcgcgcacgg gctccaccca gtcgttgagg cgttgcgcga gggccacgac aaggatcgga
     3481 ccgtcgggca ggctgctcgt ccggttcaat gcagcttggc gcacgaagcc gctgcggtgg
     3541 aacaagagga gcggggcgag cggctccggc gcgatcgcac gccagctttc ctctgtcgtg
     3601 aaccgtatga gagcctgtgg cggttgaatc gggaaatccc ggagcgcgac ggaaatcgga
     3661 tcaagggcgt cgagcgggag tgtctcgatc cgggcgacga cgcgctccag cttcagcttg
     3721 gcaagcggcg tcgagcgcgc cgctgcaatg agcgcgacaa tctcggagag tacatcgcag
     3781 atcagcccgt cgctcgccat gcggggttcc tgcgatacgg cccgatctcg aggccggact
     3841 tggcgaccat gtccggccag tcatcgccga ggagaaaggc ctgctcggca tcgcggatgg
     3901 tggtcatacc gtcgaggcct tcgagcgtcg catcgttgta gcccggatgg cagaagagga
     3961 gcgcgttctc gccggcgccc ttgatgaagg cctgaaacag ctcggccatg ggccgcttat
     4021 cggtctggta gtcgtaggca cccgagaagc cgtcattggt cggcacgccg gcgcgggcag
     4081 cagtccgccg gatgccgcgc ccgagcgagg agatcagcag tgatttggcc gtcgcgacac
     4141 ctcgtgccag gacgcgcgag gccggctcgg cgccgctgcg cacccacgtc tcgccgccac
     4201 cgacgcgctt ggcgagcctc accacgatgt cacgcacgcc ggggagctgc tgcacgtgat
     4261 gatgcccgtc gatgtgcgcg ggcggcctcc cccacacgtc gatgaagctc gcgagctggc
     4321 gctcgatctc cgcctcgatc tcgccgagcg gcaggcggcg cagcagccca gccttgaaga
     4381 ccgcgccgat cggcggcagc ttgccgtctg gcgcataggt cggcatggcg ccgagcggcg
     4441 tctggtcggt cagcgtcacg tggaggccga tgtcggcgtc tcccgccacc tcgcggagtg
     4501 ctgcggcttc ttccggccag aatcggctcg cggccatcac gctggtcgcc gagatggcgc
     4561 gccgggccag cagctcgcgg atcgcgcggc tgacgcccgg cgtgaaggcg tagtcgtcgg
     4621 cgcagacgac gaggcggcgc gtggcgctca ctccttccac ggaacgccgc ccgcgtaggt
     4681 cttgtagctg acggcggaga gccagccggc atcgacgggg agcaggatgc ccgtgatcga
     4741 gcgcgcctga tcggaagaga ggaaggcgat cgcgtcggcg atgtcggagg gctccggcag
     4801 ccggtcgagc gcgtgcacgc ccatgatctt tttcatgtcg cgctggccgc tcgcgacctt
     4861 ctcgcggagc ggcggcgtca tcacgtaggt gggcccgacg ctgttcacgc ggatgccgaa
     4921 ccggccgagc tcgacggcga gcagctgggt gagccgctcg acggcggcct tccccggatt
     4981 gtaggcggcg agcggcagcg gcaggcggct gttgatcgag ccgatggtga cgatcgtgcc
     5041 acctttcgcg gggatcatct gcttcccata gacgcgcgcc gcatacagcg tgccgttgta
     5101 gttgacggcc cacatgcgct cgtgggcgac gaggtccatc tcgaggatcg attccgagtt
     5161 cgggatgagg cccgcgcagg tgacgagcgc ggtcgctggc ccgaggtcct tcgccgtctg
     5221 agccgcgacc gcctcgactg ccttcaggtc ggacacgtcg cagcgccaga agcgcgcctt
     5281 gacgccactc ttggatagct cgaccgcaag ctttgtgccc gcgccctcgt tcatgtcgag
     5341 gatcgccacg tcgaagccgt cccgcgcgaa ccgccgcgcc gtcgcctccc cgatcccgct
     5401 cgccccaccc gtgatgacgg cgacaccctt tccagccatt tcgtgtgctc catgtgctct
     5461 tggggcgcga tgcccctcac cccgttgata gcaacgatgc ggcaggcggt cacgcgatgc
     5521 cgacgcgggc cgcgggttgg acagtgtgcg cgacatcgcc tagtcaacga tgccgccaga
     5581 tactcccgcg ccgagcccca cccgcgtgac ccttatcctt ctcgccaagc ttaccgtgac
     5641 gccggcgctc gtctggctcg cagcctatgt catgcgccga tatggtgggt tgctcgccgg
     5701 catgctggtc ggctttccgg tgatgacggc gccggtcgcc ttcttcatgg cctgggagca
     5761 gggccgggag ttcgccgagg agtctgcgct cggcattctc ctcaccgtgc cggcggtctc
     5821 gatgttcgcg atcgcctacg gcctcgcctc ccggcgcggc gggtggcgag cgggctgggc
     5881 ggccggcgtc gccgcattct tcgccgtcag cgtggcgacc gtcacgctcc gcctcgacat
     5941 cccgccggcc gcggccatcg ccgccctggt gatcgcgatc ggnnnnnnnn nnttgtacct
     6001 cgtcggccgc ccgccggtga aagcgccggc gcgtgcagca ccctggtggg atgtctggct
     6061 aaggatggcg atcaccatgg ctcttgtgat tgcggtcacc gccgcggcca atgcaatggg
     6121 tccgcgctgg agctcgatcc cggcgagcta tccggcgatc acgtcggtgg tcatcccgtt
     6181 tacgcacgcc cgtgacggtc gcgatgcggc gctggccgtg gtgcgcggca tgctgctctc
     6241 gcacatcgcg ttcgcggcac tattcgtcat cgccgcggcg ctgattgccg atcacggcgt
     6301 gatcgtcgca tatgccgttg cgacgggggc ggcgctcggc ttaagcggca tcgtcatcag
     6361 ttgcgaccgc tggctcgcac ggcggcgccc gacgacggtg tgagtagtgt cagcgccccg
     6421 tcttcacggc gcgcgccggg attccggtac caccgggcac gccttcgagg agctgtgcag
     6481 cgagcagctt gtcgaggccg accgcatcac ctgtgaactg ctgcgaccag aggatcgccg
     6541 ccgcatgcga gcctggacgc atcctgaggg ggagctgcat ggagttctgg tcgaggaggt
     6601 cgagcttgcg caccgcatcg gaaagcgatc ggcccagctc ggtcaaggca ta
//
LOCUS       DAORNH010000261         6632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8589_length_6632_cov_2.542497, whole genome shotgun sequence.
ACCESSION   DAORNH010000261 DAORNH010000000
VERSION     DAORNH010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8589_length_6632_cov_2.542497"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PK264_20165"
     CDS             complement(<1..202)
                     /locus_tag="PK264_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRD78222.1"
                     /translation="MSLKTISPADAKRLIDDGAVLVDIRGADEHARERIPGARNLPVH
                     TMVAVDSGSRPVLFHCKSGNRTN"
     gene            complement(266..583)
                     /locus_tag="PK264_20170"
     CDS             complement(266..583)
                     /locus_tag="PK264_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRD78223.1"
                     /translation="MPQAPTELADFQANAANVAGVLRALANERRLMILCKLVEWGEGN
                     VGMLAAAVGLSQSALSQHLAKMREEKLVDFRRESQTLWYRVSDPRIGGLLAHLQDHFC
                     SQR"
     gene            658..1536
                     /locus_tag="PK264_20175"
     CDS             658..1536
                     /locus_tag="PK264_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRD78224.1"
                     /translation="MLMSAPAPIITAFFDEPTNTVSYLVHDPETRRAAVIDPVLDYDH
                     RTGKASAKSADAILAAARAGGLTIDWVLETHAHADHLSGAPYIKLKTGAKVGIGEHIK
                     DVQKIFRPVFNATDISGDGTEFDHLFKDGERFAIGNLEVEVLHVPGHTPADVAFKIGD
                     AVFVGDTLFMPDYGTARADFPGGSARTLYRSVQRLLALPPETRLYMCHDYKAPGRDHY
                     AWETTVAEQRENNVHIKDGVTEDAYVAMREARDAKLAAPLLLMPSIQVNIRAGKLPPA
                     DKDGVHYLRIPVRLSA"
     gene            complement(1554..>1724)
                     /locus_tag="PK264_20180"
     CDS             complement(1554..>1724)
                     /locus_tag="PK264_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78225.1"
                     /translation="REVTDACLQYWGGAGYLWESPVARAYRDGRLTSIGGGADEIMLT
                     IIARSAGMGGRG"
     assembly_gap    1725..1736
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1737..2700)
                     /locus_tag="PK264_20185"
                     /pseudo
     CDS             complement(1737..2700)
                     /locus_tag="PK264_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965786.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(2704..2892)
                     /locus_tag="PK264_20190"
     CDS             complement(2704..2892)
                     /locus_tag="PK264_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78226.1"
                     /translation="MAEDDTRRRSPLNERKERLASALRENLQKRKQQARLRARSDDEC
                     LGDGAASRGRNTDHGKDA"
     gene            2984..4087
                     /locus_tag="PK264_20195"
     CDS             2984..4087
                     /locus_tag="PK264_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRD78227.1"
                     /translation="MTTPRYTSVVAPLPSTVPFVGPETQERARGAKFTCRLGANESVF
                     GPSPKAIAAMQQAAGDCWMYCDPENHDLKAALAAELGVKPANIVIGEGIDSLFGYTVR
                     LVTEPGDCVVSSLGAYPTFNFHVAGYGGRLVTVPYVADREDPASLIEAAKRERPKLVF
                     FANPDNPMGSWWPADAVAALIDALPAETVLVLDEAYVEFAPAGTAPPLDVGRQNVIRY
                     RTFSKAYGMAGARIAYAITEESVAKSFDKIRNHFSVNRIAQAGALAALADKAYLANVV
                     AKVARARERIVAIAREHGLSPLPSATNFVTIDCGGDGAYAKRILDGLITRGVFVRMPG
                     VAPLNRCIRVSAGTDDDLAVFARELPEALRAAR"
     gene            4332..>4622
                     /locus_tag="PK264_20200"
     CDS             4332..>4622
                     /locus_tag="PK264_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRD78228.1"
                     /translation="MSQPKELPEPAQREIARRRTFAIISHPDAGKTTLTEKLLLFGGA
                     IQLAGHVKAKKDKRSTRSDWMAIERARGISVATSVMTFEYGGAVFNLLDTPGH"
     assembly_gap    4623..4648
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4649..5939
                     /locus_tag="PK264_20205"
     CDS             <4649..5939
                     /locus_tag="PK264_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRD78229.1"
                     /translation="DDTYRTLTAVDSAIMVLDAARGIEARTRKLFEICRLRDIPIVTF
                     VNKMDRESRDPFDLLDEIEKTLALETAPMVWPIGRGRGFRGTYDLVTPRIRELDQADD
                     GRTVSGVDDPAIAALLDEDERQRWRDELDLVGAACNQLDIEAFRAGTQTPVYFGSALR
                     NFGVRDLLDSLIAYAPPPRSQTTATRMVDPKDEGLTGLVFKIQANMDPNHRDRIAFMR
                     VCSGRLKRGMKVKIVRTGRQIALQAPQFFFAQDRSLAEEAYAGDIVGIPNKGLLRIGD
                     AVTEGEDLTFSGIPSFAPEILRRVRLEDAIRAKKLRDALKELAEEGLVQLFFPLDGSP
                     PLVGVIGALQLDVMADRLKSEYDLPIAFETASFETVRWIRGKTRAAIDQFIAKHRSAI
                     GNDLDGDPVFLADTAFNLRWTAERSPDIEFVEIKTGL"
     gene            6095..>6632
                     /locus_tag="PK264_20210"
     CDS             6095..>6632
                     /locus_tag="PK264_20210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD78230.1"
                     /translation="MTTGQAPASNLRGIAAMLISQAAFIANDTMIKLAADQLPAGQAI
                     FLRGLCTCALAIMLVWLTGALAYRVHPQDRPLLGVRVIGELGSTYFYLFALFNMKIGD
                     ATAIIQFVPLAITAGAALFLGEAVGWRRWLAALVGFAGVMIVIRPGSSAFNAWSLLAI
                     VCLFSVVGRDLFTRIVGIS"
BASE COUNT         1095 a         2212 c         2133 g         1154 t
ORIGIN      
        1 gattggtgcg attgccggac ttgcagtgaa acagcaccgg acgcgagccg gaatccaccg
       61 cgaccatggt gtgaaccggc aagttgcgcg cgcctgggat gcgctcgcgc gcgtgctcgt
      121 cggcgccgcg gatatcgacg agcacggcac cgtcgtcgat cagacgcttg gcatctgcgg
      181 gtgaaatcgt cttgaggctc atgacgtgtc actttcctgg tgaatgcgaa ttggttcggt
      241 ggtccgaccc gctacgtacg gttggtcagc gctgagaaca gaagtgatcc tggagatggg
      301 cgagcaatcc gccgatgcgc ggatcgctga cgcgatacca gagcgtctgg ctctcgcgcc
      361 tgaagtcgac gagcttctcc tcgcgcatct tggcgagatg ctgggagagt gccgactgcg
      421 agaggcccac cgccgcagcg agcatgccga cgttgccctc gccccattcg acgagcttgc
      481 agaggatcat cagacggcgc tcgttggcga gcgcgcgcaa cacgcctgcg acgttggcgg
      541 cgttggcctg gaagtcggcg agctcggtcg gggcctgcgg cattcgatgc cctccaatac
      601 gttcgccctt gttaaataag caatctctaa tatatagtca acagcagtcg caaggagatg
      661 ctcatgtccg cccctgctcc catcatcacc gccttcttcg acgagccgac caatactgtc
      721 agctatctcg tgcacgatcc cgagacgagg cgggcggcgg tcatcgatcc cgtgctcgac
      781 tacgaccacc gcacgggcaa ggcctccgcc aagtcggcag atgccattct cgcagcggcg
      841 cgggcgggcg gcctcacgat cgactgggtg ctcgagacgc atgcgcacgc cgatcatctg
      901 tccggcgcgc cctacatcaa gctgaagacc ggcgccaagg tcggcatcgg cgagcacatc
      961 aaggacgtgc agaagatttt tcgtccggtg ttcaacgcga ccgacatctc cggggacggc
     1021 accgagttcg accacctgtt caaggacggc gagcgcttcg cgatcggcaa tctcgaggtg
     1081 gaggtgctgc acgtgccggg ccacactccg gctgacgtcg ccttcaagat cggcgatgcc
     1141 gtgttcgtcg gcgatacgct cttcatgccg gactacggca cggcccgtgc cgattttccg
     1201 ggcggcagcg cccgcacgct ctatcgctcc gtgcagcggc tcctggcgct gccgcccgag
     1261 acgcggctct acatgtgtca cgactacaag gcgcccgggc gcgatcacta tgcgtgggag
     1321 acgaccgtcg ccgagcagcg cgagaacaat gtgcacatca aggacggcgt gacggaggac
     1381 gcctatgtcg cgatgcgcga ggcacgtgat gccaagctcg cggcgccgct tctcctcatg
     1441 ccatcgatcc aggtgaacat ccgcgccggc aagctgccgc cggcggacaa ggacggcgtg
     1501 cactatctgc gcatccccgt ccggctgagc gcctgataca ccaacgctac cgttcagcct
     1561 cgaccaccca tgccggcgct gcgggcgatg atcgtcagca tgatctcgtc cgcgccgccg
     1621 ccgatcgagg tgaggcggcc gtcgcgatag gcgcgcgcga cggggctctc ccagagatag
     1681 ccggcgccgc cccaatattg gaggcaggcg tcggtcacct cgcgnnnnnn nnnnnntcag
     1741 cttcgccatc gaggcgaggc gcgtcacgtc ctcgcccgcg atcatcagcg cgccggcccg
     1801 gtaggtgagg gcccgcagcg cctcaacctc ggtcaggagc tcagcgaggc ggaactgcac
     1861 ggcctggttg tcgaggatcg agcggctgaa ggccttgcgc tcgctcgtgt aggcgacggt
     1921 tgcggcaatc acgcgatcga gcatgccgca cttgtttgcg gcggcccaca gccgctcttc
     1981 ctggaactgc tccatctggt ggaggaagcc gcgtccctcg atgccgatgc ggttcgactt
     2041 cggcacgcgc acgtcgtcga agaagagctg cgcggtatcg gaggcccaca tgccgagctt
     2101 cttgagtttc ttcacgcggg tcacgccctt cgcgtcgagc ggcacgcaga cgagcgactt
     2161 gttcttgtgt gggccgccgt ccggcgaggt attgacgagg aggcagatcc aatcggcctg
     2221 catgccgttg gtgatccaca tcttcgagcc gttgatcacg tagtcgccgc cgtcgtggcg
     2281 ggcggtcgac tggatcgaag cgacgtcgga gccggcgccg gcctcagaga cgccgatcgc
     2341 ggccacccgc tcgccggcga tcacgggcgc caggaactcg cgccggagcg catcgctgcc
     2401 gaagcgggcc agcgccggcg tcgccatgtc ggtctgcacg ccgatcgaca tcggtatcgc
     2461 gccgcaatcg atcctcccga gcgcctcggc gagtatgatc gcccacgaat agtcgagtgc
     2521 ctggccaccg tattcggcgg gcttggtgag gccgaggaga ccgaggcggg caagcttcgg
     2581 gaagagttca tgtgccggga actgctccgc ctcctcccag gcatcgacgt gcgggttcac
     2641 ctcggcgtcg atgaagcgct cgattgtcga gcgcagcgcc gcgtgctcgg ccgtcagcat
     2701 ggatcaggcg tccttcccgt ggtctgtgtt gcggcctcgc gacgctgctc catcgcccag
     2761 gcactcgtcg tcgctccggg cacgaaggcg cgcctgctgc ttgcgcttct gcaggttctc
     2821 gcggagcgcg ctcgcgaggc gctccttgcg ctcgttcagc gggcttcgcc tgcgtgtgtc
     2881 gtcttccgcc atgctccggc accttgtcgc aatgaggtga ttggcgggct gagactaaca
     2941 tgacaaaaga agcctcacac aggcatctga ggcccagctc tgcatgacca cgccccgcta
     3001 tacgtccgtc gtcgccccgt tgccctcgac cgtgccgttc gtcggtcccg agacgcagga
     3061 gcgggcgcgc ggcgcaaaat tcacctgccg gctcggcgcc aacgagagtg tgttcgggcc
     3121 ctcgcccaag gcgattgccg ccatgcagca ggcggccggc gactgctgga tgtactgcga
     3181 tcccgaaaac catgacctga aggccgcgct cgcggccgaa ctcggcgtga agcccgccaa
     3241 catcgtcatc ggagagggca tcgattcgct cttcggctac actgtccgtc tcgtcaccga
     3301 gcccggcgat tgcgtggtga gctcgctcgg cgcttacccg acgttcaatt tccatgttgc
     3361 gggctatggc ggccggctcg tcacggtgcc gtatgtcgcc gatcgcgagg acccggcatc
     3421 actcatcgag gcggccaaac gcgagcggcc caagctcgtg ttctttgcca accccgacaa
     3481 cccgatggga agctggtggc cggccgacgc agtagcggcg cttattgacg ctttgcctgc
     3541 ggaaacggtg ctagtgctcg acgaggccta cgtcgagttt gcgccggccg gaaccgcgcc
     3601 gccgctggat gtcggccggc agaacgtgat ccgctaccgc acattctcga aggcctacgg
     3661 catggccggc gcgcgcatcg cctatgccat caccgaggag agtgtcgcga agagcttcga
     3721 caagatccgc aaccacttct cggtcaaccg catcgcgcag gcgggcgcgc tcgcggcgct
     3781 cgccgacaag gcataccttg caaacgtggt cgccaaagtt gcgcgggcac gcgagcggat
     3841 tgtcgccatc gcccgcgagc acggcctttc gccgctgccg tcggccacaa acttcgtcac
     3901 catcgattgt ggcggcgacg gcgcctatgc caagcgcatc ctcgacggcc tgattacccg
     3961 cggcgtattc gtgcggatgc cgggcgtcgc gccgctcaat cgctgcatcc gcgtctcggc
     4021 aggcaccgac gacgatcttg ccgtctttgc gcgagagctc ccggaggctt tgcgggcggc
     4081 ccggtaaacg tctcaggttc gcttcgcgat cgggctgccc gcaaatcctc gcatactcga
     4141 tcttgtttca cacgaacggg aggttcggag ggtgtccctc cgactggggt acggggcgag
     4201 cgcgccgttc gcgcagcgac ggcactgcgc cccgtgaggc gagcgacttt gcgcgcccat
     4261 cgaactgatg tatccgggca caaagcatgg cgcgcacccg cgccggcccc caataacgtg
     4321 atgatcgcgc gatgtctcaa ccgaaagagc tgcccgagcc cgcacagcgc gagatcgcgc
     4381 ggcggcgcac gttcgcgatc atctcgcatc cggacgccgg caagacgacg ctcaccgaga
     4441 agctcctctt gttcggcggc gcgatccagc tcgccggtca cgtcaaggcc aagaaggaca
     4501 agcgttcgac ccgctccgac tggatggcga tcgagcgggc gcgcggcatc tcagtcgcga
     4561 cctcggtgat gaccttcgag tatggcggcg ccgtcttcaa tctgctcgac acgcccggtc
     4621 acnnnnnnnn nnnnnnnnnn nnnnnnnngg acgacaccta ccgcacgctg accgcggtcg
     4681 attccgccat catggtgctc gacgcggcac gcggtatcga ggcgaggacg cggaagctct
     4741 tcgagatctg ccggctgcgc gacatcccga tcgtcacctt cgtcaacaag atggaccgcg
     4801 agagccgcga tccattcgac ctcctcgacg agatcgagaa gacgctagca ctcgagacgg
     4861 cgccgatggt gtggccgatc gggcgcggtc gcggcttccg cggcacctac gatctcgtca
     4921 cgccgcgcat tcgcgagctc gaccaggccg acgacgggcg caccgtctcg ggcgtcgatg
     4981 atccggcgat cgcggcactg ctcgacgagg acgagcgcca acgctggcgc gacgaactcg
     5041 acctcgtcgg cgcggcttgc aatcagctcg acatcgaggc cttccgggcg ggcacgcaga
     5101 cacccgtcta cttcggcagc gcgctccgca atttcggcgt gcgtgatctc cttgattcgc
     5161 tcatcgccta tgcgccgccg ccgcggagcc agacgacggc aacccgaatg gtggatccca
     5221 aggacgaagg gctgaccggc ctcgtcttca agatccaggc gaacatggac cccaatcacc
     5281 gcgaccgcat cgccttcatg cgcgtctgct cgggccgctt gaagcgcggc atgaaggtga
     5341 agatcgtccg cacgggccgc cagatcgcgc ttcaggcgcc gcagttcttc tttgcccagg
     5401 accgttcgct cgccgaggag gcctatgccg gcgacatcgt cggcatcccg aacaaggggc
     5461 tcttgcgcat cggcgatgcc gtgaccgagg gcgaggacct aaccttcagc ggcatcccga
     5521 gcttcgcgcc ggaaatcctg cgccgcgtgc gcctggagga tgcgatccgg gccaagaagc
     5581 tccgcgacgc cctgaaggag cttgccgagg aaggcctcgt ccaacttttc ttcccgctcg
     5641 atggctcgcc gccgctggtt ggcgtgatcg gcgcgctgca gctcgacgtg atggccgacc
     5701 gcttgaagag cgagtacgac ctgccgatcg ccttcgagac ggcctcgttc gagacggtgc
     5761 gctggattcg cgggaagacg cgggcggcga tcgaccagtt catcgccaag caccgctccg
     5821 cgatcggcaa cgacctcgac ggcgatccgg tgtttcttgc cgatacggcc ttcaatctca
     5881 ggtggacggc cgagcgcagc ccggacatcg agttcgtcga gatcaagacg ggactttgat
     5941 cggcgccgtg catgaacagg cggatggcag actcgcggaa agtgcgcagg ccattcgacg
     6001 ctgattgatc gacacggccc ctgtcttcga ggcatgctgg cggtcgcgtc gagagttgaa
     6061 gccgtcccgc aatcgcgaga agacctcccg gatcatgacc acaggtcaag cgcctgcgag
     6121 caacctgcgc ggtatcgcgg cgatgctcat ctcccaggcg gccttcatcg cgaacgatac
     6181 gatgatcaag ctcgcggccg accagctgcc ggcgggacag gcgattttct tgcgcgggct
     6241 gtgtacctgc gcgctcgcca tcatgctcgt ctggcttacg ggtgcgctcg cctatcgagt
     6301 gcatccgcag gacaggccgc tactcggcgt gcgcgtcatc ggcgaacttg gctcgaccta
     6361 cttctatctc ttcgcgctgt tcaatatgaa gattggcgac gcgacggcca tcatccagtt
     6421 cgtgccgctc gccatcacgg cgggggcggc actctttctc ggcgaggcgg tcggatggcg
     6481 gcgttggctc gcggcgctcg tcgggttcgc cggcgtcatg atcgtcatcc ggccggggtc
     6541 gtcggccttc aacgcctggt cgctgctcgc catcgtctgc ctgttcagcg tggtgggccg
     6601 cgatcttttc acccgtatcg tcgggatttc cg
//
LOCUS       DAORNH010000262         6591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8656_length_6591_cov_2.615667, whole genome shotgun sequence.
ACCESSION   DAORNH010000262 DAORNH010000000
VERSION     DAORNH010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8656_length_6591_cov_2.615667"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2033)
                     /locus_tag="PK264_20215"
     CDS             complement(<1..2033)
                     /locus_tag="PK264_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HRD78231.1"
                     /translation="MRAILRRLREIMAEPGDGQSRLNHIVQQIAGFMVAEVCSIYLKR
                     QDGMLELFATEGLNPGAVHNTFMRRGEGLVGRCAELAAPVNEPDAQSHPAFSYRPETG
                     EEPFHSFLAVPILRAGDVLGVLTVQNKTAKEYSDEELEVLETTAMVLAEHLVSGAVSG
                     TNTAAAQSRAVGHIIKAPAIAEGIALGHVVLHEPRVVVTETAADDPVAEALRLDLALD
                     ELRGNLDELLDQDAIAHAGEHRDVLEAYRMFARDRGWQRRLREAVATGLNAEAAVELV
                     QNQQRARMLRQADHFWRERLRDLDELSDRLMRILAARRDGGSTRKELPADAVVVARSM
                     GAADLLDYDRAKLRGLIIEDGGDASHVAIVARALGIAAVGQARGIIERVDPGDAIIVD
                     AQSGEIYVRPSSEVVAAYTDKVRFLAGRQKAYASLRGLPAQSKDGIRVDLQLNSGLLV
                     DMAHLEATGADGVGLFRTELQFMVASTFPRLERQTELYRSVLDAAGGKPVIFRTLDIG
                     ADKILPYLRHQEEPNPALGWRGVRMGLDRQALLRIQLRALIKAAGDREISLMLPMVAT
                     AFEVDAARGLLDREIELHVRRGGTPPARIHFGIMIEVPSVLYDLDTILRKVDFVSVGS
                     NDLMQYLFAADRDNERVANRFDVLSPPALRALAAIPEAAARHRIPARLCGEIGG"
     gene            complement(2219..3481)
                     /locus_tag="PK264_20220"
     CDS             complement(2219..3481)
                     /locus_tag="PK264_20220"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRD78232.1"
                     /translation="MAVLVMKFGGTSVANVERIRNVARHVKREVDAGNKIAVVVSAMS
                     GTTNQLVAWVREASLLHDAREYDAVVASGENITAGLLAIVLQSMGIQARSWQGWQIPV
                     VTNAAHGAARIIDIKGEKLRERLDQGEVAVVTGFQGIEPERNRLATLGRGGSDTSAVA
                     LAVALKADLCDIYTDVDGVYTTDPRIVPKARRLPKISYEEMLEMASLGSKVLQTRSVE
                     LAMVHRMRTRVLSSFVAPEAMEPARPETWDKIGTIVCDEDEIVEQQVVSGIAYAKDEA
                     KVTLLAIEDKPGVAARIFGPLAEANVNVDMIVQNLSSDGRRTDMTFTVQEAELPRTLE
                     VLKAARADIGDFEIKSSADVVKVSVIGIGMRSHAGVAAQMFKALASKGINILAITTSE
                     IKISVLIDSAYAELAIRTLHSLYGLDAD"
     gene            complement(3650..3970)
                     /locus_tag="PK264_20225"
     CDS             complement(3650..3970)
                     /locus_tag="PK264_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRD78233.1"
                     /translation="MVKITYVQPDGSKQTVDAEVGMTVMETAKKHNISGIEAECGGAC
                     ACATCHVYVDPAWQAKTGKPSGMEEDMLDFAFDVRDESRLSCQIKVVTELDGLTVKVP
                     EKQF"
     gene            complement(4039..5274)
                     /locus_tag="PK264_20230"
     CDS             complement(4039..5274)
                     /locus_tag="PK264_20230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD78234.1"
                     /translation="MSTLKAPLAGIRILDLTQFLSGPFGTQILGDLGADVIKVEAPDG
                     DITRRLPPHFVAGDSCYFHSINRNKRSIAIDLKSKDGQALLRRLALECDVVIENWRPG
                     VMARLGLDYSDLVAERPALISCSISGFGQDGPYRDRPAYDMIAQALSGGMSMTGEPGG
                     RPVRSGIPLGDLAGGLYGVIGILAAIIEARASGKGRFIDVSMLDCQIAMLTYQAAYHL
                     KSGTVPGLQGAAHDSIPTYRTFTAADGRDVVVCANTERMWQGLARALGKPELIEEPLF
                     KTNELRHQNRASLLPILEAAFRTAPAATWVERLQAEEIPTCAVNTVADSLADSQVRHR
                     GMVLTLKDRDGTPLTVAGDPLKLRDAAGRLADQPANFPPKLNQDANGVLSELLGLSSE
                     TIADLHRTGVIGARTAGKA"
     gene            complement(5289..5609)
                     /locus_tag="PK264_20235"
     CDS             complement(5289..5609)
                     /locus_tag="PK264_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRD78235.1"
                     /translation="MKDIMKIMKQAQEMQARMQQMQADLSALEIEGQSGAGMVRVTLN
                     GKGEVRAVKIDPKLMVAGEGEILEDLVVAAFRDAKAKVESAAAAKMQEITAGLPLPPG
                     MKLF"
     gene            complement(5639..>6591)
                     /locus_tag="PK264_20240"
     CDS             complement(5639..>6591)
                     /locus_tag="PK264_20240"
                     /inference="COORDINATES: protein motif:HMM:NF023780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRD78236.1"
                     /translation="LTLLGALHRDGADPIEVLGDLAEAIHQTTRVKVAGPTGLGDAYP
                     EEERRRAQALAGRLSLAYLSRAWQMLVRGIDEAGRAPSTTAAAEMVVIRLAHVSDLPS
                     PDDVIRIIGGQSSARPRGSVGAGETAPAGTDRAAPTSSVSARVDSDDDIPFTPSENPR
                     GDDIEADLGDGLDDPTDGTPLAAAPVEITTFEDVLELISRHRDVKLKVHLEENTSLVK
                     FDPAGSIELHLLAHAPKELANELREKLNIWSGRRWMVALSRQPGARPIAEIRREREAA
                     ERAKLEGHPAVRAILDGFPDTRITEIRPLKREPDEDAAAS"
BASE COUNT         1000 a         2081 c         2366 g         1144 t
ORIGIN      
        1 ccgccgatct cgccgcagag gcgcgccgga atgcgatggc gggcggcggc ctcggggatc
       61 gccgcgagcg cccgcaaggc cggcggggag aggacgtcga agcgattggc gacgcgctcg
      121 ttgtcgcggt cggcggcgaa taggtactgc atgaggtcgt tgctgccgac ggaaacgaaa
      181 tcgactttcc gcaggatcgt gtcgagatcg tagagcaccg acggcacctc gatcatgatg
      241 ccgaagtgga tgcgggcggg cggtgtgcct ccgcgcctga catgcaactc gatctcgcga
      301 tcgagcaggc cgcgcgccgc gtccacctcg aaggccgtgg cgaccatcgg cagcatcagg
      361 ctgatctcgc gatcgcctgc ggctttgatg agggcgcgca gctggatgcg cagcagcgcc
      421 tggcggtcga ggcccatgcg cacgccgcgc cagccgaggg cggggttcgg ctcctcctgg
      481 tggcgcaggt atgggaggat cttgtccgcg ccgatatcga gcgtgcggaa gatgaccggt
      541 ttgccgcccg ccgcgtcgag cacggagcgg tagagttcgg tctggcgctc aaggcgcggg
      601 aaggtcgagg cgaccatgaa ctgcagctcg gtgcggaaga ggccgacccc gtcggcgccc
      661 gtggcctcga ggtgcgccat gtcgacgagg aggccggagt tgagctggag atcgacccgg
      721 atgccgtcct tggactgggc cggcagcccg cggagcgaag cgtatgcctt ctgccggccc
      781 gccaggaagc ggactttgtc ggtataggcc gcgacgacct cagaggaggg gcggacgtag
      841 atctcgccgg actgggcgtc gacgatgatg gcgtcgcccg gatcgacgcg ctcgatgatg
      901 ccgcgtgcct ggccgacggc cgcgatcccg agcgcgcggg cgacgatggc gacgtggctc
      961 gcgtctcctc cgtcctcgat gatgaggcca cgcagcttcg cgcggtcgta gtcgagaagg
     1021 tcggctgccc ccatggagcg ggcaacgacg acggcgtcgg ccgggagttc cttgcgcgtg
     1081 gaaccgccat cgcggcgcgc ggcgagaatg cgcatcaggc gatcggagag ctcgtcgaga
     1141 tcgcggagcc gctcgcgcca gaagtggtcg gcctggcgga gcatgcgggc gcgctgctgg
     1201 ttctggacga gttcgacggc ggcctcggcg ttgaggccgg tcgctacggc ctcgcggaga
     1261 cggcgctgcc agccgcggtc gcgcgcgaac atgcggtagg cctcgagcac gtcgcggtgc
     1321 tcgccggcat gcgcgatggc gtcctggtcg agcaactcgt ccagattgcc gcgcagctca
     1381 tcgagcgcca ggtcgaggcg gagcgcctcg gcgacgggat cgtcggccgc ggtttcggtg
     1441 acgacgacgc gcggttcgtg cagcaccaca tggccgagcg cgatcccctc ggcgatggcc
     1501 ggcgccttga tgatgtggcc gacggcgcgg ctttgagccg cggcggtatt ggtgcccgag
     1561 acggcgccag agacgaggtg ctcggcgagc accatcgccg tcgtctcgag tacctcgagc
     1621 tcctcgtcgg aatattcctt cgccgtcttg ttttggacgg tgaggacgcc gagcacgtcg
     1681 cccgccctca ggatcggtac ggcgaggaag gagtggaacg gctcctcgcc ggtctcgggg
     1741 cgataggaaa aggccggatg gctttgcgca tccggctcgt tcacgggtgc agcgagctcg
     1801 gcgcaacgcc cgacgaggcc ttcgccgcgg cgcatgaatg tgttgtgcac ggcgccgggg
     1861 ttcagcccct ccgtcgcgaa cagctcgagc attccgtcct ggcgcttgag gtagatcgag
     1921 cagacctcgg ccaccatgaa gccggcgatc tgctgcacga tgtgattgag ccgggactgg
     1981 ccgtcgccgg gctccgccat gatctcgcgc agccggcgca ggatggcgcg catcgccggc
     2041 tcccgggaga cggggcgtgg tcctggcggc ggggtacgag gcttcgacat gggaccctga
     2101 tcgaacgtgg ctgctgttac ggcagcggca ggcattttgc catgtccgcc cctcagttgg
     2161 cccgcccgat ctgaatgcag tccggcgggg tggttagcgc cggctgccgg gtttaccgtc
     2221 aatcggcatc gaggccatag agggagtgga gggttcggat ggcgagctcg gcgtaggctg
     2281 agtcgatgag tacgctgatt ttgatctcgg acgtcgtgat tgccaggatg ttgatacctt
     2341 tgctcgcgag tgccttgaac atctgcgcgg cgacgccggc atgacttcgc atgccgatgc
     2401 cgatcacgga gaccttgacg acgtcggccg aactcttgat ctcgaagtcg ccgatgtcgg
     2461 cgcgggccgc cttcagcacc tcgagggttc ggggcagctc ggcctcctgc acggtgaacg
     2521 tcatgtccgt ccgccggccg tcggaggaga ggttctggac gatcatgtcg acgttcacgt
     2581 tggcctcggc cagcgggccg aagatgcgtg cggcgacacc cggcttgtcc tcgatggcaa
     2641 ggagggtgac ctttgcctca tccttcgcgt aggcaatgcc gctgacgacc tgctgttcca
     2701 cgatctcgtc ctcgtcgcaa acgattgtgc cgatcttgtc ccacgtctcg gggcgcgcgg
     2761 gctccatggc ctccggcgcc acgaagctcg agagcactcg ggtgcgcatg cgatgcacca
     2821 tggcaagctc caccgagcgc gtctggagca ccttggagcc gagggaggcc atctcgagca
     2881 tctcctcgta ggagatcttg gggagccggc gcgctttcgg cacgatgcgc ggatccgtcg
     2941 tatagacgcc atcgacgtcg gtgtagatgt cgcagagatc ggccttgagc gctactgcca
     3001 aggcaacggc gctggtatcc gagccgccgc gcccgagtgt cgccaggcga ttgcgctcgg
     3061 gctcgatgcc ctggaagccc gtgaccaccg caacctcgcc ctggtcgagg cgctcgcgca
     3121 gcttctctcc cttgatgtcg atgatgcgcg cggcaccgtg cgcggcgttg gtgacgacgg
     3181 ggatttgcca gccctgccag gagcgcgcct ggatacccat cgactggagg acgatggcga
     3241 ggagaccggc cgtgatgttc tctccggatg cgacgacggc gtcatactcg cgcgcgtcat
     3301 ggaggaggga cgcctcacgc acccaggcga ccagctggtt ggtcgtcccc gacatggccg
     3361 agacgacgac ggcgatctta ttgccggcat cgacctcgcg cttcacgtgg cgggcgacat
     3421 tgcgaatgcg ctccacgttg gcgaccgagg tgccgccgaa cttcattacg agaacagcca
     3481 tgttcgacca gccctcagcg caacgcccgg gtcggtcgcc gcggtgcatc ctatggagtt
     3541 gccccaaggg cgctcagaca cgattggtcc gcgccgcgtc gagcgacggc atctcttatc
     3601 gacaacacct gccgtccgca acctcccgtc acgggccggt ggtgaaatgt cagaactgct
     3661 tctctggcac cttgaccgtg aggccgtcga gctcggtcac gaccttgatc tgacagctca
     3721 aacggctttc atctcgcaca tcgaaggcga aatcgagcat atcctcctcc atgccggagg
     3781 gcttgcccgt cttggcctgc caggccggat cgacatagac gtggcaggtg gcgcaggcgc
     3841 aggcgccgcc gcactcggcc tcgatccccg agatgttgtg cttcttcgcc gtctccatca
     3901 ccgtcatgcc gacctcggca tcgaccgtct gcttcgaccc gtccggctgc acgtaagtga
     3961 tcttgaccat tgcgagaagg ctcccgtagt gcgcttcgag cgcagcgagc gccgatgtag
     4021 tccggcgcgg agccgaattc aagccttgcc ggccgtgcgc gctccaatga cgccggtcct
     4081 gtgcaggtcg gcgatcgttt cgctggagag gccaagcagc tccgagagca ccccgttcgc
     4141 gtcctggttg agcttcggcg ggaagttcgc aggctggtca gccagcctac cggcggcgtc
     4201 acggagcttc agtgggtcgc cggcgacggt gagcggggtg ccgtcgcggt ctttcagggt
     4261 gagcaccatg ccgcggtggc gcacctgcga atcggcaaga ctgtcggcta ccgtgttgac
     4321 ggcgcaggtc gggatttcct cggcctgcag ccgctcgacc caggtggcag caggcgccgt
     4381 gcggaatgct gcttccagta tggggaggag gctcgcgcgg ttctggtggc ggagctcgtt
     4441 ggtcttgaag agcggctcct cgatcagctc gggcttgccg agggcgcggg cgaggccctg
     4501 ccacatgcgc tcggtgttgg cacatacgac gacgtcgcgg ccgtcagctg cggtgaaggt
     4561 gcggtaggtc gggatcgaat catgagcggc gccctgcaga cccggaacgg tgcctgattt
     4621 caggtggtag gcggcctgat acgtcagcat ggcgatctgg cagtcgagca tcgagacgtc
     4681 gatgaagcgg cccttgccgc tcgcccgcgc ctcgatgatg gcggcgagga tgccgatcac
     4741 accgtagagc ccgccggcga gatcgcccaa ggggatgccg gagcgcaccg gtcggccgcc
     4801 cggctcgccg gtcatcgaca tgccgcccga gagggcctgc gcgatcatgt cgtaggcggg
     4861 gcggtcgcgg taggggccgt cctggccgaa gccggagatc gagcacgaga tgagtgcggg
     4921 gcgctcggca acgaggtcgg aatagtcgag gccgagccgc gccatgacgc ccggccgcca
     4981 gttctcgatg acgacgtcgc attcgagcgc gaggcggcgc agcagcgcct ggccgtcctt
     5041 gctcttcagg tcgatcgcga tcgagcgctt gtttcgattg atcgagtgga aatagcagct
     5101 gtcgccggcg acgaagtgcg ggggcagccg gcgcgtaatg tcgccgtccg gggcctcgac
     5161 cttgatcacg tcggctccga gatcaccgag gatctgcgtg ccgaaaggtc ccgagagaaa
     5221 ttgcgtgaga tcaagaatgc ggatgccggc gagcggcgcc ttgagcgtcg acatgaaggg
     5281 gtgccgcctc agaatagctt catgcctggc ggcagcggga gacccgcggt gatctcctgc
     5341 atcttggctg cagctgcgct ctcgaccttg gccttggcgt cgcggaaggc tgcaacgacg
     5401 agatcctcga ggatctcgcc ttcgcccgcg accatcagct tcggatcgat cttcacggcg
     5461 cgcacttcac ccttgccatt tagcgtgacg cggaccatgc cggcgcccga ctggccttcg
     5521 atctcgagcg ctgaaagatc cgcttgcatc tgctgcatgc gggcctgcat ctcctgcgcc
     5581 tgcttcatga tcttcatgat gtctttcacg actcactcca tgtgtggcgc gccctgcgtc
     5641 agctggcggc ggcatcctcg tcgggctcgc gtttcaaagg acggatctcg gtgatgcggg
     5701 tgtccgggaa gccgtcgagg atggcgcgga cggcgggatg gccctcgagc tttgcccgtt
     5761 cggcggcttc ccgctcgcgc cggatttccg cgatcggacg ggcacccggc tggcgcgaga
     5821 gagcgaccat ccagcgccgg ccgctccaaa tgttgagttt ctcgcggagc tcgttggcga
     5881 gttctttggg ggcatgcgcg aggagatgca actcgatcga gcccgcggga tcgaatttga
     5941 cgaggctcgt attctcctcg aggtgcacct tgagcttcac gtcgcggtgg cggctgatga
     6001 gctcgagcac gtcctcgaac gtggtgatct cgaccggggc cgcggcgagg ggcgtgccgt
     6061 ccgtaggatc gtcgagtccg tcgccgaggt cggcttcgat gtcgtcgccg cgcgggttct
     6121 ccgagggcgt gaaggggatg tcgtcgtcgc tgtcgacgcg tgccgagacg ctcgacgtcg
     6181 gtgcggcgcg atctgtgcca gcgggagctg tctctcccgc gccaacggag cctcgcggcc
     6241 gcgcggacga ctgcccgccg atgatgcgga tcacgtcgtc gggcgagggg aggtcggaga
     6301 cgtgggcgag gcggatgacg accatctcgg cggcggctgt cgtgctcggc gcgcgccccg
     6361 cctcgtcgat gccgcggacc agcatctgcc aggcacgcga gagataggcg agcgagaggc
     6421 gtccggcgag ggcctgggcg cggcggcgct cctcctctgg gtaggcgtcg ccgaggcccg
     6481 tcggaccggc gaccttgacg cgcgtcgtct ggtggatggc ctcggcgaga tcgccgagca
     6541 cctcgatcgg atcggcgccg tcgcgatgca gggcgccgag cagtgtcagg g
//
LOCUS       DAORNH010000263         6578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8681_length_6578_cov_2.717615, whole genome shotgun sequence.
ACCESSION   DAORNH010000263 DAORNH010000000
VERSION     DAORNH010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8681_length_6578_cov_2.717615"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>215)
                     /locus_tag="PK264_20245"
     CDS             complement(<1..>215)
                     /locus_tag="PK264_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRD78237.1"
                     /translation="EVWCPADKIPTHFEASLEGLEIGRSIHISAVKLPEGVKPTITDR
                     DFTVATIAGAAALKSDADEAAATPGAEG"
     assembly_gap    216..233
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<234..603)
                     /locus_tag="PK264_20250"
     CDS             complement(<234..603)
                     /locus_tag="PK264_20250"
                     /inference="COORDINATES: protein motif:HMM:NF013545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRD78238.1"
                     /translation="MAQAAIKIMARPGAGKGAARAARRAGHVPGIIYGGDEKTPEAVS
                     VDFKELSNTISKGRFFSSVFDLDLDGRKVRAIPRAVQFDPVKDFPIHVDFQRIGKDNR
                     VRLMIPMRFTNDQLSPGIKRG"
     gene            complement(823..1119)
                     /locus_tag="PK264_20255"
     CDS             complement(823..1119)
                     /locus_tag="PK264_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HRD78239.1"
                     /translation="MKTYEGVRAFDGLKVTVDGRELSPHYEVKQFTTWGFEWTYEGES
                     PQQLALAILYDHLGDAPRAIRLSETFMRKIIANLDNDWQLTSAEVEAAVKEMGG"
     gene            1364..2035
                     /gene="rplS"
                     /locus_tag="PK264_20260"
     CDS             1364..2035
                     /gene="rplS"
                     /locus_tag="PK264_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRD78240.1"
                     /translation="MNIIQQLEQEQIKSITAKRGVPEFGPGDTLKVMVKVIEGEGKDQ
                     TVRLQAYEGVCIARSGGGLNESFTVRKISYGEGVERVFPIYSPFVAEIEVLRRGKVRR
                     AKLYYLRGRRGRAARIFERSDARARRLAQAWKGFKKPKGEIEDLTQIKSIDADLAARL
                     KQLNCVKIEQIANFSDDDIANVDEALKLGGRIEREGWIDQAKRLVAEAAAAEVPAEGE
                     EKPKA"
     gene            2231..3196
                     /locus_tag="PK264_20265"
     CDS             2231..3196
                     /locus_tag="PK264_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD78241.1"
                     /translation="MQPARPGASEAAAPAGERRVAPVRQDSLRGILLVCVAFALFSGL
                     DATAKYMATVVQLPVTQVTWIRFVSQFLLIVLVMGIVQVPRLLSTVKLKQQLVRSVLM
                     MASTVMNFLALRHLRLDQTQTIYFLAPFVVALLAGPFLGEWVGWRRLVAIMVGFSGII
                     VVVRPGVATFEPAMLFAFGSMLAYASFMLLTRYLASYDPPEVTLFYSLLAGTYFLAPL
                     AIVDWVWPESVFIWLLLGSMGLWGGLGHYIFILAYRRAPASTLAPFVYLGLVTHVAAG
                     YLVFNQVPDAWTLAGAGIVIASGIYLVHREHVTRRAKARSERADT"
     gene            3292..4728
                     /locus_tag="PK264_20270"
     CDS             3292..4728
                     /locus_tag="PK264_20270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRD78242.1"
                     /translation="MRRLKRLALLLTLLGPLTAMASAAMAPAAMAQGRTEVRIAENVY
                     VVPDKNAEAITGWLIVKAGCADEAYGDCRGLAHYLEHLLLVGRRPDHTTSSFSLFPEG
                     VANGWTSHRTTTYFQRFPVKGAEDAERVDKLLAYFMRLVAELKIADDAADRERNVVLQ
                     EYNVNTGRNPFARFSIELEKSLLPDEALGQRVIGSPSTIKGFTVAAAADFHRHWYTKG
                     NIAIVLHGPIDPAVIRPIAERHLASLPDRPNPPARYALISRPYPHAITKLRHSDPEAK
                     QTGVYVYRVTGFAEIDRRQVAAARRIVNAFLASKLPGSPNDLLIEKQGLALTIGIGST
                     KVRDGVSRLSISGQPVAGIAPDKLAEAALAYVEGLAGSGLSAEIIERLKTRLAVGQDL
                     VDKQPAELAGSLVGWLSNHLSYDDWLWRRTALEQVTPQSVQLVLQTHAARGRTVVGII
                     TPPSAAATPTATPGPSAPAAPAPTKAKP"
     gene            4776..6146
                     /locus_tag="PK264_20275"
     CDS             4776..6146
                     /locus_tag="PK264_20275"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRD78243.1"
                     /translation="MPIPTRLLLVLLTLLATREPAYAETPFMEMKTPGGLTFHYRFDD
                     RSPYAALHFGWRDGFIDRHKGKEGLATLFHAMVSQAADGEGGHAFSERLADLHARVVF
                     ANSWTASYAQVRAPAAQLGEAVIAALDALAKAKPGEPLLKRLRDRARGSEAQSLVRSE
                     VIAERAAMRALLGDHRVTRAYDNARFDRIGAADLDLWRGERLSRAGLLIVASGALPAE
                     TYASLIDKAFLPLPERPEPVRVAPAPPKALLRTIVVERPGTQTILLLAAPMMLADVRD
                     GPTLAIASSILGGGSQGRLFKAVRGDLGATYGASASVVTVHKEQRIVLIRASVANDLA
                     KPSLAALRDTYAKWHAEGMTDAEITPARERSITGVKAAWRDISSSSSWLLGLLAEDRL
                     PADMVEHEGRLKAITRESLNLMTRETFPSLDKFLTIIVAPKADGFAADCVIAIDTEVE
                     RCRPRH"
     gene            6305..>6578
                     /locus_tag="PK264_20280"
     CDS             6305..>6578
                     /locus_tag="PK264_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78244.1"
                     /translation="MADAGLSRRSLLAMGLSLPALLLGNTDAVAGRGLLRSATSWAYQ
                     LQGGIGGLASSGADVIVVDADHHRGAASRLRTKPGGGRRAVLAHLSI"
BASE COUNT         1048 a         2298 c         2083 g         1131 t
ORIGIN      
        1 ccttcggcgc ccggcgtcgc cgccgcctcg tcggcgtccg acttcagcgc ggctgcgccc
       61 gcgatcgtcg ccacagtgaa atcgcgatcg gtgatggtcg gcttgacacc ctcgggcagc
      121 ttcacggcag agatgtgaat cgagcggccg atctcgaggc cctcgaggct cgcctcgaaa
      181 tgggtcggga tcttgtcggc ggggcaccac acctcnnnnn nnnnnnnnnn nnncgccgcg
      241 cttgatgccc ggcgagagct gatcgttcgt gaacctcatc gggatcatca ggcggacgcg
      301 gttgtccttg ccgatgcgct ggaagtcgac gtggatcggg aaatccttga cggggtcgaa
      361 ctgcacggcg cgcgggatcg cccggacctt gcggccgtcg aggtcgagat cgaagacgga
      421 cgagaagaag cgtcccttcg agatcgtgtt cgacagctcc ttgaagtcga cagacaccgc
      481 ttccggcgtc ttctcgtcgc caccgtaaat gatgcccgga acgtgtccgg cgcgacgtgc
      541 tgcccgtgcg gctcccttgc cggcaccggg gcgcgccatg atcttgatcg cggcctgagc
      601 catgtgtgtg tctccaatgg gaacagccgc ccgcgccccc gttccggcac gacgccggcc
      661 agaagcccaa acggccaaag cccgccagcc tccagggtgc cgcgcggacg accggcttat
      721 agctagaggt agggcggatg gcaaatgggg tgtcatgccg gtgacatgcc gccggtgaca
      781 tgccggtggc ccaattcagt gacttggtca gtcaaccggc catcacccgc ccatctcctt
      841 caccgccgcc tccacttcgg cgctggtcag ctgccagtcg ttgtcgagat tggcgatgat
      901 cttgcgcatg aaggtttcgg agagtcggat ggcacggggg gcgtcgccca ggtggtcgta
      961 gaggatcgcg agcgccagct gctgcgggct ttcgccctcg taggtccact cgaagcccca
     1021 agtcgtgaat tgcttgacct cgtaatgcgg tgacagctcc cggccatcca ccgtcacctt
     1081 gaggccgtcg aacgcccgca cgccttcgta ggtcttcatg gtcatggctc tttgtggctc
     1141 tgccccgact gcactcgatc cggatgatat atctcaccgg tgtttcctgt cgacaggcgc
     1201 gccggcttcg ggtatcagcc aaccaccgcc ttaagcggat ttgctggtgt cctcggccac
     1261 cggccgcacg aaccgttggc tccgggcgca atcgtctccc cggcgtatcg gctcctctgc
     1321 atcacgcctc gagagaatca atcaagacca acgggccaga gccatgaaca ttatccagca
     1381 gctcgagcaa gagcagatca agtccatcac ggcgaagcgc ggcgtgccgg agttcggccc
     1441 cggcgatacg ctcaaggtga tggtgaaggt gatcgagggc gagggcaagg accagacggt
     1501 gcgcctccag gcctacgagg gcgtgtgcat cgcgcgctcg ggcggcggcc tcaacgagag
     1561 cttcacggtc cgcaagatct cctacggcga gggcgtcgag cgcgtcttcc cgatctactc
     1621 acccttcgtc gccgagatcg aggtgctgcg ccgcggcaag gtgcgccgcg ccaagctcta
     1681 ttatctccgt ggtcgccgcg gtcgcgccgc ccgcatcttc gagcgctcgg acgcgcgcgc
     1741 ccgccgcctc gcgcaggcct ggaagggctt caagaagccg aagggcgaga tcgaggatct
     1801 cacgcagatc aagtcgatcg acgccgatct cgcggcgcgg ctcaagcagc tcaattgcgt
     1861 gaagatcgag cagatcgcca acttctccga tgacgatatc gccaacgtcg atgaggcgct
     1921 gaagcttggg ggccgcatcg agcgcgaggg ttggatcgat caggcgaagc gcctcgtcgc
     1981 cgaagccgcc gccgccgagg tgccggccga gggcgaggag aagcccaagg cctgagtttt
     2041 cgacctcgaa ttggtgagat gcgaagcccg gtccgagcgg ccgggctttt tcatgagaac
     2101 catgcgcaag cttcatgggg atcgctgcgt gtccggtcta cgatcgcgtc actgtttttt
     2161 cgcgaatgcg ttatagctca gcgctcctcg tccgcgctga tgccgtgcgg gccgagagca
     2221 gcggaacacc atgcaaccag cgagaccagg cgcgagcgag gcggcggcac cggccggcga
     2281 acgccgtgtc gcccccgtcc gccaggacag cctgcgcggc atcctgctcg tgtgcgtcgc
     2341 cttcgccctc ttctcggggc tcgatgcgac ggcgaagtac atggcgacgg tcgtgcagct
     2401 gcccgtcacg caggtgacct ggatccgctt cgtctcgcag ttcctgctga tcgtcctcgt
     2461 catgggcatc gtgcaggtgc cgcggctgct ctccacggtg aagctcaagc agcagctcgt
     2521 ccgctcggtg ctgatgatgg cctcgacggt gatgaacttc ctggcactgc gccatctccg
     2581 cctcgaccag acgcagacaa tctattttct cgccccgttc gtcgtcgcgc tgctcgccgg
     2641 gccctttctc ggcgagtggg tcggatggcg gcggctcgtt gccatcatgg tcggcttctc
     2701 ggggatcatc gtcgtcgtgc gcccgggagt tgcgacattc gagccggcca tgctgttcgc
     2761 cttcggctcg atgctcgcct atgcctcgtt catgctgctg acgcgctatc tcgcgagcta
     2821 cgatccgcct gaagtgacgc tcttctactc gctgctcgcc ggcacttact ttctcgcccc
     2881 gctcgcgatc gtcgactggg tctggcccga gagcgtgttc atctggctgc tgctcggatc
     2941 gatgggactg tggggcggcc tcggccacta catcttcatc ctggcctacc ggcgcgcgcc
     3001 ggcgtccacg ctcgccccgt tcgtgtacct cggcctcgtc acgcacgtcg cggcggggta
     3061 cctcgtgttc aaccaggtgc cggatgcttg gacactcgcg ggcgccggca tcgtcatcgc
     3121 ctcggggatc tatctcgtgc atcgcgagca cgtgacgcgc agggccaagg cccgctccga
     3181 gcgcgccgat acctgacggc aacgccgctg cccgcgattg gtcgcaatcc aaccgtctga
     3241 tacgagtttc actatcgccg cagccaatct cgtcgccgcg aaggatccac aatgcgtcgc
     3301 ttgaagcgcc ttgcactgct gttgacgctg ttgggtccgc tcacggccat ggcctcggcg
     3361 gcgatggccc cagcggcgat ggcgcaaggg cgcaccgagg tgcgcatcgc cgagaacgtc
     3421 tacgtcgtcc cggacaagaa cgccgaggcc atcaccggct ggctgatcgt caaggcgggg
     3481 tgcgccgacg aggcctatgg cgattgccgc ggccttgccc actacctcga gcatctcctg
     3541 ctcgtcggtc gccgccccga tcacacgacg tcgagcttca gcctttttcc cgaaggcgtc
     3601 gccaacggct ggacgtccca ccgcaccacg acctacttcc agcgcttccc cgtgaagggc
     3661 gccgaggacg cggagcgggt ggacaagctg ctcgcctact tcatgcgcct ggtcgcggaa
     3721 ctgaagatcg ccgatgacgc cgccgatcgc gagcgcaacg tcgtgctgca ggagtacaac
     3781 gtcaacacgg gccgcaaccc ctttgcgcgc ttctcgatcg aactcgagaa gtccctgctg
     3841 cccgatgagg cgctcggcca gcgcgtcatt ggctcaccgt cgaccatcaa gggtttcacg
     3901 gtggctgctg ccgccgactt ccatcgccac tggtacacga aagggaatat cgcgatcgtg
     3961 ctgcacgggc cgatcgatcc cgcggtcatc cgcccgatcg ccgagcgcca cctcgcgtcg
     4021 ctccccgaca ggccgaaccc acctgcccgc tacgcactga tctcgcggcc ctacccgcac
     4081 gcgatcacca agctccgcca cagcgatccg gaagccaagc agaccggagt ctacgtctat
     4141 cgcgtcacgg gctttgccga gatcgaccgg cgccaggtcg ccgcggcgcg acggatcgtc
     4201 aacgccttcc tcgcgtccaa gctgccggga agcccaaatg acttgctcat cgagaagcag
     4261 gggctcgctc tcaccatcgg catcggctcg accaaggtgc gcgacggcgt ctcgcgcctg
     4321 tcgatctcgg gccagcctgt cgccggaatc gcgcccgaca aactcgccga agccgcgctc
     4381 gcctacgtcg aggggctggc cggcagcggc ctctccgccg agattatcga gcggctgaag
     4441 acgcgcctcg ccgtcggcca ggacctcgtc gacaagcagc cggcggaact cgcaggctcg
     4501 ctcgtcggct ggctgtcgaa tcacttgagc tacgacgact ggctctggcg gcggaccgcc
     4561 ctcgagcaag tgacgccgca atcggtgcaa ctcgtcctgc agacgcatgc ggcgcgcgga
     4621 cggaccgtcg tcggcatcat cacgccgcca tcggccgcgg caacgccgac tgccacaccg
     4681 gggccgagcg ctcctgcggc gccggcaccc accaaggcga aaccctgacg ccacgcgcca
     4741 tccgacccat cgccgccaga cctcgcgagc gccccatgcc aattccgacc cgactgctgc
     4801 tggttctcct gaccctcctg gcgacgcgcg agcccgcgta tgccgaaacg cccttcatgg
     4861 aaatgaagac gccaggcggc ctcacctttc attatcgttt cgacgaccgc tcgccgtacg
     4921 ccgccctgca tttcggctgg cgtgacggct tcatcgatcg ccacaagggc aaggagggcc
     4981 tcgcgaccct gttccacgcc atggtgagcc aggcggcgga cggcgaaggc gggcacgcct
     5041 tcagcgaacg tctggccgat ctgcatgcgc gggtcgtgtt cgccaactcc tggacagcga
     5101 gctatgccca ggtcagggcg ccggccgccc agcttggcga agccgtgatc gcggctctcg
     5161 acgccctggc gaaggcgaaa ccgggcgagc cgctcctgaa gcggctccgc gaccgcgctc
     5221 gcgggagcga ggcgcagagc cttgtccgtt ccgaggtgat cgccgaacgc gccgccatgc
     5281 gggcgctcct cggtgatcac cgcgtgacgc gggcttatga caatgctcgt ttcgaccgga
     5341 tcggcgccgc cgacctcgat ctgtggcgcg gcgagcggct ctcgcgggcc gggctcctga
     5401 tcgtcgcgtc cggtgcgctg ccggccgaaa cctacgcgag cctcatcgac aaggccttcc
     5461 tgccgctccc ggagcgccca gagccggtcc gcgtcgcacc ggcgccgccg aaggccctcc
     5521 tgcgtaccat cgtcgtcgag cgacccggca cgcagacgat cctgctgctc gccgccccta
     5581 tgatgcttgc cgatgtccgc gacggtccga cgctggcgat cgcgagttcc attctcggcg
     5641 gcggctcaca gggccgcctg ttcaaggccg ttcgcggcga tctcggcgcc acctatggcg
     5701 cctccgcgtc cgtcgtcacc gtccacaagg agcagcgcat cgtcctgatc cgcgcctctg
     5761 tcgccaacga tctcgccaag ccgtcactcg cggctctgcg cgacacctat gcgaagtggc
     5821 acgccgaggg catgacggac gccgagatca cgcccgcgcg cgagcgctcg atcacgggcg
     5881 tcaaggcggc ctggcgcgat atctcgagct ccagttcctg gctgctcggg ctcctcgcgg
     5941 aggaccgcct gcccgccgac atggtcgagc acgagggccg cctcaaggcg atcacccgcg
     6001 agtcgctcaa cctgatgacg cgcgagacct ttccctcact cgacaagttc ctgaccatca
     6061 tagtcgcacc caaggctgac ggcttcgccg ccgactgcgt gatcgccatc gacaccgagg
     6121 tcgagcgatg ccgccctcgg cactgaggaa gcactcgcct cgacatggtt aagaaaatta
     6181 accatttcag ccatcgaggg gggacgtgag gagggagttc gggcagtagg ggccagttgt
     6241 tgaaccaact gaggccagaa ttatgcacga cacacctcgg atcttgcgcg cggaggatgc
     6301 cggcatggcg gatgccggtc tttcgcggcg ctccctcctc gccatgggac tttcgctgcc
     6361 ggcgctactg ctgggcaata ccgacgcggt tgcaggccgt gggctcctgc gtagtgcaac
     6421 gagctgggcc taccagctgc agggcggcat cggcgggctc gcctcgtccg gcgccgacgt
     6481 catcgtcgtc gatgccgacc accatcgggg cgccgcgagc cgcctgcgga ccaagccggg
     6541 cggcggccgt cgcgcggtgc tcgcccatct ctcgatcg
//
LOCUS       DAORNH010000264         6563 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8704_length_6563_cov_2.409035, whole genome shotgun sequence.
ACCESSION   DAORNH010000264 DAORNH010000000
VERSION     DAORNH010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8704_length_6563_cov_2.409035"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PK264_20285"
     CDS             <1..187
                     /locus_tag="PK264_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78245.1"
                     /translation="STDFVALNHSGYAENSAIMKDIELLLRTGLRPPEQRMPTIAKIG
                     TPTGEFWRYPAVRTGAP"
     gene            complement(243..318)
                     /locus_tag="PK264_20290"
     tRNA            complement(243..318)
                     /locus_tag="PK264_20290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(283..285),aa:Thr,seq:tgt)
     gene            complement(397..481)
                     /locus_tag="PK264_20295"
     tRNA            complement(397..481)
                     /locus_tag="PK264_20295"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Tyr,seq:gta)
     gene            complement(582..1157)
                     /locus_tag="PK264_20300"
     CDS             complement(582..1157)
                     /locus_tag="PK264_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HRD78246.1"
                     /translation="MSNATIDLLLQRRSVKAAMMTSPGPSAAELETILRAASRVPDHK
                     KVEPWRFIVFEGEARARFGDLLATACAAEEKEPPSPARLDMERQRFLRAPLVIGVVSQ
                     VRQVAGAPEWEQVLSCGAAIMNLVIATNALGFGSCWITEWYAYSPAVRSGLGLAEHER
                     IAGFVYIGTAKERQGDRDRPDLARIVSRFPG"
     gene            1272..>2744
                     /locus_tag="PK264_20305"
     CDS             1272..>2744
                     /locus_tag="PK264_20305"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRD78247.1"
                     /translation="MSPLPESDRAGVRTPCRAVGRGLLALLATVAVAAVLSRGALHAE
                     APSQPTALLLVDGSRSMWGTLDGDRVHKIYQVRNAVRQVLPKLAPTMRVGLASFGHRR
                     AGECTDIEVIVPPTAGPDGGGERIVSILDKFNPRGRGPIADGLRAAAQALKGEAGAKS
                     IVLIHDDPDNCKGDPCEAAVEIKAAQPGLVIHVIALGPKREEMQRLRCVSNSTGGRYV
                     EAASQAALLAALEETITVALGAAGSPTASSLPPRTAAPQPGPAGPLKPKADTDKRPGL
                     HLMTTLVSGGPPVDLPVRWRVMREGAQDVPIYEELAVNPVLLVRPGRYTVELDVGIPP
                     ILRSTFIVGANEATLGPMVLDAGLVRVRPVLHKSGPTVEGASIVVQRAGEPNAPVVLI
                     EVSRDRPAMLLLKAGTYNVSAGLGLAKQSRTVTVAPGRQDDLELVLGTGILRVALATR
                     EGGRPIDTALFTVLEDDPDAPQGRREVARSAAATSAITALL"
     assembly_gap    2745..2754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2755..3360
                     /locus_tag="PK264_20310"
     CDS             <2755..3360
                     /locus_tag="PK264_20310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78248.1"
                     /translation="FALAAGTYYVVARHGYSEARERVALKAGETIERVLVAPTAGLTL
                     RTKVAGAATIVPELVSYRLQRLDITDQDPVITSAPEARYALAAGRYRIEGRYGLLNAR
                     VVREIELKAGQDQQLLLEHQAALLTLKLAEGGVLPGAGDAYWEIRDQRGTLVWATGQS
                     EPRAPLLPGRYNVRVEQREKSGETTVELRSGETRAIDVAPK"
     gene            3357..4409
                     /locus_tag="PK264_20315"
     CDS             3357..4409
                     /locus_tag="PK264_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78249.1"
                     /translation="MIESRIGGIVVELSRAGRAGLLALVVSVVMAAGAAEAQQDGRTT
                     GWGTEVDAARKDAPPPSPPAPASGWESKASPPPAAVPNTTVVPRTTPDSTTPMGEVQL
                     IAQLTADGERIDQGIVWRVYAAKSGSDGKPRLIALHREAAPTVRLAAGEYVVNSAFGR
                     AHLTRKVTVKAGPPAIETFVLNAGGLRVQAVLPGGGPVQDRTVSYEIYAGERNQSGER
                     DLIMSAVKPGVVIRLNAGPYHIVSTYGDANSIVRTEVAVEAGKLTEAVVSHAAARIAF
                     RLVERAGGEALPDTSWTIETQQGAVVKAAVAAITSHILAPGAYRVTARHGGRTLRRDF
                     TVTAGVAAMVEVVVAE"
     gene            4697..5329
                     /locus_tag="PK264_20320"
     CDS             4697..5329
                     /locus_tag="PK264_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HRD78250.1"
                     /translation="MKSNALKSIRLDAEWASRTILPIEFDGEEVQLEAHAYQGKDPTC
                     QALALVNRGNPGDDCIGTVRVHSGCVTGDIFHSLRCDCYPQLKASMREITRTPNGIMI
                     YVPYHEGRGIGLVKKIQAYALQDKGLDTVDANVAVGAPIDARDYELAADILFDLGFPE
                     IRLMTNNPAKVQALRDQGIRIVEQLPVISTPSRHNVRYLQTKKERMAHKL"
     gene            complement(5412..>6563)
                     /locus_tag="PK264_20325"
     CDS             complement(5412..>6563)
                     /locus_tag="PK264_20325"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HRD78251.1"
                     /translation="NDLFGFEAQALELAANALELLLHHGPRLARRGDRPLFLRARDGL
                     ELGHALLELVFFRGDDGGRLFGRDLAAHLCAGDGLFGLALTSSWLIWSWKGVIWALAA
                     IVGLVMLAPRVPPEAVMGMFKARRLDPGRGSELTRILEAIAVRAGLPAAPGLYIIPST
                     TLNAFATGNERRSAIAVTEGLLRRLTLREVAGVLAHEVSHIKNRDLLVMMIADAITRA
                     AQVFSYIAVMLTVFNLLAWMAEGEPVSWAAIAILYLAPTASSLLQLGLSRTREYDADL
                     DGAMLTGDPEGLASALRRLEQYQGRFWEDMMLPVPARRVPQPSILRTHPATEERIQRL
                     AEVMRPEAIARRGGLRQPPLIVIEAPIVTLIGFSPSELRPRYRWPGIWY"
BASE COUNT         1127 a         2141 c         2244 g         1041 t
ORIGIN      
        1 ctcgacggac ttcgtcgcgc tcaaccactc aggctacgcc gagaactctg cgatcatgaa
       61 ggacatcgag ctcctgctgc gcacgggcct gcgcccgccc gagcagcgca tgcccacgat
      121 cgccaagatc ggcacgccga ccggcgagtt ctggcgctat ccggcggtgc ggaccggcgc
      181 gccctgaacg ggcgtcctca gtcgaaatgg cgagcatgtg tccggagcgg accggctgtg
      241 agtggtgccg gctgagggac ttgaaccccc gacctaccgc ttacaaggcg gttgctctac
      301 cagctgagct aagccggctc acatcagtgc cgcatgctgg atcacgccag ggcggatcaa
      361 gtgccgcggg cgcgctccgc caggagcgaa cgcggatggt ggagggggct ggattcgaac
      421 cagcgtaggc tatgccaacg gatttacagt ccgtcccctt tagccactcg ggcacccctc
      481 cactcagagt tcgtcgcgac cgaagccgcg gcggccgact tatggggaga cccgtccgga
      541 tagtcaatcg aaaacgcgcc gaaaacaggc gtgtcgcgcg gtcagcccgg gaagcgcgag
      601 acgatgcggg cgaggtcggg ccggtcgcga tcgccctgac gctccttcgc ggtgccgatg
      661 taaacgaaac cggcgatgcg ctcatgctcg gcgaggccga ggccactccg cacggcgggg
      721 ctgtaggcgt accactccgt gatccagcag ctgccgaagc cgagcgcgtt cgtggcgatg
      781 acgaggttca tgatcgcggc cccgcaggag agcacctgct cccactcggg cgcgcctgca
      841 acttgccgaa cctgcgagac gaccccgatc acgagcggcg ccctgaggaa gcgctggcgc
      901 tccatgtcga ggcgggcagg cgacggcggc tccttctcct ccgcggcaca ggccgtcgcg
      961 agcagatcgc cgaagcgcgc tcgggcctcg ccctcgaaca cgatgaagcg ccacggttcc
     1021 acctttttgt ggtcgggcac gcggctcgcc gctctcagga tggtttcgag ctccgcggcg
     1081 ctcggtcccg ggcttgtcat catcgcggcc ttgacggagc ggcgctgaag cagcagatcg
     1141 atagtcgcat tactcattgc gtcgcccgat gcggtggctt ggttgaagtt gcatattggg
     1201 tctcgctatc tcacttttgc cccgactaca atggaacctg ctcgctcgaa ctgtgacgct
     1261 ggaccctcgc catgagccca ctgcctgaaa gcgaccgagc tggtgtccgc acgccatgcc
     1321 gtgccgtcgg tcgcggcttg ctcgcgctgc tggcgacagt cgctgtcgca gctgtcctct
     1381 cgcgcggcgc actccatgcg gaggctccaa gccagccgac agcgctcctg ctcgtcgacg
     1441 gctcgcgctc gatgtggggc acactcgacg gcgatcgtgt ccacaagatc taccaggtac
     1501 gcaacgccgt ccgccaggtg ctgccgaagc ttgcgccgac catgcgtgtc ggcctcgcct
     1561 cgttcgggca tcgcagggcc ggagagtgca ccgacatcga ggtgatcgtc ccgccgacgg
     1621 cagggccgga cggcggcggc gagcgcatcg tctcgatcct cgacaagttc aatccgcgtg
     1681 ggcgcggtcc gatcgcggat ggtctcaggg ccgcagcgca ggccttgaaa ggggaggcag
     1741 gggccaaatc aatcgttctc attcacgacg atcccgacaa ttgcaaaggc gacccctgcg
     1801 aggccgcagt ggagatcaag gcggcgcaac cgggccttgt gatccacgtg atcgcgctcg
     1861 gcccgaaacg cgaggagatg cagcggttgc gctgcgtctc caactcgacc ggcgggcgct
     1921 acgtcgaggc ggcctctcag gcagcgctcc ttgcggctct cgaggagacg attaccgtgg
     1981 cgctcggtgc ggccggcagt ccaacggcat cctccctgcc gcccaggaca gcagcgcctc
     2041 aacccggtcc cgccggcccg ctgaaaccga aggcggacac cgacaagcgc ccaggcctcc
     2101 atctcatgac gacactcgtc tcgggcggac cgccagtcga tcttcctgtg cgctggcgcg
     2161 tcatgcgcga gggggcgcag gacgtgccga tctatgagga gcttgccgtc aaccctgtgc
     2221 tgctcgtcag gccggggcgc tacaccgtcg aactcgacgt cggcattcca ccgattctgc
     2281 gatcgacctt catcgtcggc gccaatgagg cgacactcgg gccgatggtc ctcgatgccg
     2341 gtctggtacg cgtccggcct gtgctgcaca agtcggggcc gactgtcgaa ggcgcctcga
     2401 tcgtcgtgca gcgtgctggc gagcccaacg cgccggtggt cctgatcgag gtctcgcgcg
     2461 accggccggc gatgctgctg ctcaaggccg gaacgtacaa cgtgagtgcc gggctcggcc
     2521 tggcgaagca atcgcggact gtgactgtcg cgccaggccg ccaggacgac ctcgagctcg
     2581 ttctcggcac cggcatcctc cgggtcgcgc tcgccacgcg tgagggcggg cggccgatcg
     2641 acactgccct cttcacagtg ctcgaggatg atcccgacgc cccgcagggt cggcgcgagg
     2701 tggcccgctc ggcggccgcc acctccgcca tcaccgcctt gctgnnnnnn nnnnttcgca
     2761 ttggcggccg gcacctacta tgtggtcgcg cggcacggct attccgaggc ccgtgagcgc
     2821 gtcgcgctca aggccggcga gacgatcgag cgcgtgcttg tcgcgccgac cgcggggctc
     2881 acgctgcgca ccaaggtcgc cggagctgcg acgatcgtgc cggaactcgt cagctatcga
     2941 ctccagcggc tcgacatcac tgatcaggat ccggtgatca cgagcgcgcc cgaggcgcgc
     3001 tacgcactcg cggcggggcg ctaccggatc gaaggccgct acggcctcct caatgcgcgc
     3061 gtcgtgcgag agatcgagct caaggcgggg caggatcagc agctgctgct cgagcatcag
     3121 gcagctctcc tcacgctgaa gctcgctgaa ggtggggtgt tgccgggggc cggagatgcc
     3181 tactgggaaa ttcgcgatca gcgcggtacg ctcgtgtggg caaccgggca gagtgagccg
     3241 cgggcgccgc tgcttcctgg ccgctacaat gtacgcgtcg agcagcgcga aaaatccggc
     3301 gagacgacag tggagctccg gtcgggcgag acacgggcaa tcgatgtggc gccgaaatga
     3361 tcgaaagcag gatcggtggc atcgtggtcg agctgtcgcg ggcggggcgt gccggcctgc
     3421 tcgcgctggt cgtgtcggtc gtcatggcgg ccggcgcggc cgaggcgcag caggacggcc
     3481 gcacgacggg ctggggcacc gaggttgacg ccgcgcgcaa ggatgcgccc ccgccatcac
     3541 cgcctgcgcc ggcctcgggc tgggaatcga aggcctcgcc tccgcctgca gccgttccca
     3601 atacgacggt cgtgccccgg accactcccg attcgacaac gccgatgggc gaggtgcagc
     3661 tcattgcgca gctgaccgca gacggcgagc gcattgatca aggcatcgtc tggcgtgtct
     3721 acgcggcgaa atcgggttcc gacggcaagc cgaggcttat cgccctacac cgggaggcag
     3781 caccgaccgt gcggctcgcg gcaggagagt acgtcgtcaa ctccgcgttc ggccgtgcac
     3841 atctgacgcg caaggtgacc gtcaaggccg gcccgccggc gatcgagacg ttcgtcctga
     3901 atgccggtgg gctgcgtgtg caggccgtgc tgccgggagg tggtcccgtg caggatcgga
     3961 ccgtcagcta cgagatctac gccggcgagc gcaatcagtc gggcgaacgc gacctgataa
     4021 tgtcagcggt gaagccgggt gttgtgatcc gcctcaatgc gggtccctat cacatcgtca
     4081 gcacctacgg agatgccaac tcgatcgtgc ggaccgaggt cgcggtcgaa gcaggcaagc
     4141 tcaccgaggc cgtcgtcagc catgcggcgg cgcggatcgc cttccgcctc gtcgagcgtg
     4201 ccggcggaga ggcgctgccg gatacgagtt ggacgatcga gacgcagcag ggcgcggtgg
     4261 tgaaggccgc ggttgccgcc attacatccc acatcctggc acccggggcc tatcgggtga
     4321 cggctcgcca cggcgggcgg acgctgcggc gcgacttcac cgtcacggcg ggggttgcag
     4381 ccatggttga ggttgtcgtg gccgagtagg tctcctgcta cgctgtcgac tgtcgcgcaa
     4441 ttttccattt tcattttttg tgcaatgcac tatatgcctg taaggcaacg cttaatttcc
     4501 gctgcggaga cgacataaag ccgagcaatg ctagcggctt gggcgcgatg aacgcggcgt
     4561 cgtctgaatg ttcagctgcc gttcaggtcg gaaacgctca tgtaacggac accggcggac
     4621 cgagggaatt gtcatcgcct ttacaagcga actgaagtca gcttgatggc ggcggacttg
     4681 ccgtttggag agtataatga aatcgaacgc actgaagtcg atccggctcg atgccgagtg
     4741 ggcgtcacgg actatcctgc cgatcgagtt cgacggtgag gaggtccagc tcgaggcaca
     4801 tgcctaccag ggcaaggatc cgacctgcca ggcgctggcg ctggtcaatc gcggcaatcc
     4861 gggggatgac tgcatcggga cggtacgcgt gcatagcggc tgcgtcactg gcgacatttt
     4921 tcattcgctg cgctgcgatt gctacccgca gctgaaggcg tccatgcgcg agatcacacg
     4981 caccccgaac gggatcatga tctacgtgcc ctaccacgag ggtcgcggca tcgggctcgt
     5041 caagaagatc caggcctacg cgctgcagga caagggcctc gacacggtcg atgccaacgt
     5101 cgcggtcggt gcaccgatcg acgcgcggga ctatgagctc gccgccgaca tcctgttcga
     5161 tctcggcttc ccagagattc gcctgatgac caacaatccg gccaaggtcc aggcgctgcg
     5221 cgatcagggc attcggatcg tcgagcagct ccctgtcatc tcgacgccga gccggcacaa
     5281 tgtccgctac ctgcagacga agaaggagcg gatggcgcac aagctctgag agcgcgcgtg
     5341 tgccctggtg cacgtggccg attcgagttc gagagcccct gccgatcccc ggtgggggtt
     5401 ttcactttcg ctcaatacca gatgcccggc caacggtagc gcgggcggag ttcggacggg
     5461 ctgaagccga tgagcgtcac gatcggagcc tcgatgacga tcaagggcgg ctgccgcagg
     5521 ccaccacgcc gcgcgatggc ttcggggcgc atcacctcgg cgaggcgctg gatccgctcc
     5581 tcggtcgcgg ggtgggtgcg caaaatcgac ggctgcggga cgcggcgcgc ggggacgggc
     5641 agcatcatgt cctcccagaa gcggccctga tattgctcaa ggcgacgcag cgcgctggcg
     5701 aggccctccg gatcgccggt cagcatagcg ccatcaaggt cggcgtcgta ctcgcgcgtc
     5761 cgcgacagcc cgagttggag gaggctagag gccgtcggcg cgagatagag gatggcgatc
     5821 gccgcccagg agaccggctc cccctccgcc atccaggcca gcagattaaa cacggtgagc
     5881 atgacggcga tgtaggagaa aacctgcgcg gcgcgcgtaa tggcatcggc gatcatcatc
     5941 accaggagat cgcggttctt gatgtggctc acctcgtggg cgagcacgcc agcgacctcg
     6001 cgcaacgtga gacgccgcag caggccttcc gtcacggcga ttgccgaacg tcgctcgttg
     6061 cctgtggcga acgcgttgag cgtggtgctc gggatgatgt agaggccggg ggccgcaggc
     6121 aggcccgcgc gcaccgcgat cgcctcgaga attcgggtga gctcgctgcc gcgaccggga
     6181 tcgagccggc gcgccttgaa catgcccatg acggcctcgg gcggcacgcg cggcgccagc
     6241 atgacgaggc cgacgatggc agcgagcgcc cagatcacgc ctttccagga ccagatcagc
     6301 caggacgacg tgagcgcgag gccgaagagg ccatcgccgg cgcacagatg cgcggcaagg
     6361 tcgcggccga acaggcggcc gccatcatcg ccacgaaaga aaacgagctc gagcagcgcg
     6421 tggccgagtt cgaggccgtc gcgcgctcgg aggaagagcg gacggtcgcc gcggcgcgcg
     6481 aggcgcgggc catgatggag cagcagctcc agggcgttcg ccgcgagctc gagcgcctgc
     6541 gcctcgaagc cgaagaggtc att
//
LOCUS       DAORNH010000265         6562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8706_length_6562_cov_2.628861, whole genome shotgun sequence.
ACCESSION   DAORNH010000265 DAORNH010000000
VERSION     DAORNH010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8706_length_6562_cov_2.628861"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PK264_20330"
     CDS             <1..905
                     /locus_tag="PK264_20330"
                     /inference="COORDINATES: protein motif:HMM:NF019174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HRD78252.1"
                     /translation="LEGRLNSLALAHDLASRNEPMELRQLLETELGPFDSEATGRIDL
                     RGPAVWFSQHAASVMALVVHELATNAAKYGALSRPEGSIGVSWHLANDGTCDISWVEQ
                     SPVPVSVVARTGFGTLIITRQIPFELGGSSEMSFTPRGLVARLRIPAASIALPPRTST
                     GRPLHASAATADATAQLLAGRTVLVVEDNLIVALRFEQALKHLGADRIVIAGTLEQAM
                     TLAMSDSVSLVVLDINLRGTRSYAVADTLMERDVPFFFATGYGIDADLPERFKHIPIV
                     PKPSTEAALLRTITSVLGELRPAG"
     gene            1067..1747
                     /locus_tag="PK264_20335"
     CDS             1067..1747
                     /locus_tag="PK264_20335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD78253.1"
                     /translation="MASTANRISVALVDDEAAFLFELSQRLMDHGLTVRGYRDAATLL
                     SDMQKGTTYDCIVADVRMPGLSGLELQQRLNEGSLEVPLILFTGYGDVDIAVSAMKAG
                     AWDFIGKPIAVEHLCSSVTSAVIDARQRAAERREHAAIAARVAELSERHRQVLDLMVQ
                     GLTSKQIASTLKINHRTVENYRAFVMDRIGVGNIAQLVRVMLQMRFAQNTVSAGQKQP
                     SGTGSERA"
     gene            complement(1931..2020)
                     /locus_tag="PK264_20340"
     tRNA            complement(1931..2020)
                     /locus_tag="PK264_20340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1984..1986),aa:Ser,seq:gga)
     gene            2293..>2530
                     /locus_tag="PK264_20345"
     CDS             2293..>2530
                     /locus_tag="PK264_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78254.1"
                     /translation="MTAPRAHAGEPHIALSKPSADEIKTTTCFMCACRCGIRVHLKDG
                     KVRYIDGNRDHPVNKGVICGKGSAGIMQHTSPARL"
     assembly_gap    2531..2656
                     /estimated_length=126
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2657..5118
                     /locus_tag="PK264_20350"
     CDS             <2657..5118
                     /locus_tag="PK264_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HRD78255.1"
                     /translation="SQALTGWFAQQYGTPNFAAHGGFCSVNMAAAGLYTFGGAFWEFG
                     DPDWDRTEYFMLFGVAEDHASNPIKTALGKLKKRGVKFVAVNPVRTGYNAIADEWIGI
                     EPGKDGLFVAGLIHELLRTQKIDLPSLVRYTNAPWLVIDAPGSVDDGLFARGENGKPL
                     AHDKSSGAIVDASRVDIAPSLAGTFKLADGRSARPAFELIARRFAAPETAPEAIEQEL
                     GIPALTIRRIAAEIAHAAFEKEVVLDQPWTDWAGRRHEKMIGRPVSFHAMRGISAHSN
                     GFQTCRLIHLMQILLGSIDCPGGFRYKAPFPRPTPPPNRPAGKHGTVKPMTPLAGAPL
                     GFPQGPEDLLLDPDGRPMRIDKAYSWDAPIAAHGLMHMVITNAANHDPYPIDVLFMYM
                     ANMSWNSSMNIPAMLKHLTEKDAASGEYKIPKIIYSDAYFSEMIPYADLILPDTTYLE
                     RWDCISMLDRPISEPDGPADAIRHPVVEPDRDVRAFQSVIIDLGARLKLPGFVKDDGS
                     PKFPGGYADYIVNHERGPGIGPLAGYRGADGKAYGKGAPNPKQLEQYIANGSFYQYHL
                     APEQRYFKHANKAYLDWAKDVGFIGSADPIVFQLYLEPLQKFRLAALGHGTIKVPETH
                     RVRIERHFDPIPFWYPTFGEAPAASGARYPFHAITQRPMHMYHSWGSQNAWLRQITTA
                     NRLFMSASEALKLGIRDDDWVWLASETGRIKCQVKTMQGLNPGTVWTWNAIGKRAGAW
                     GLAPDAPEATHGFLLNHLITELLPEQPGGYRFSNSDPITGQAAWYDLRVSITKASPGE
                     AAQSEPRFERLALAVPTGGAR"
     gene            5115..5873
                     /locus_tag="PK264_20355"
     CDS             5115..5873
                     /locus_tag="PK264_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRD78256.1"
                     /translation="MTSLPAPSAKKLGLVIDLDTCVGCQACATSCKEWNAEGYPAPLS
                     DEDAYGADPHGTWLNRVHSYDVGAGAESRTVHFPRSCLHCETPACVTVCPTGASYKRA
                     EDGIVLVNPDTCIGCKLCSWACAYGAREYDYTHGVMKKCTLCVDRIYNETFEPEDRIP
                     ACVRACPTGARHFGDLGDRNSEVSKLVAERAGFDLLPQSGYKPVNKYLPPRPRRDATT
                     TAGSGPSANAAATGTPTGSVADRLFAWADRMLSR"
     gene            5892..>6562
                     /locus_tag="PK264_20360"
     CDS             5892..>6562
                     /locus_tag="PK264_20360"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HRD78257.1"
                     /translation="MHPAYSVIAFTTASGAGYGLLVWLAVWGTFADLPQGWRAALVAS
                     GLALGLVSAGLLSSTAHLGRPERAWRAFSQWRTSWLSREGVAAIVTFVPAGLFALKWL
                     IDGRIGSTTAAIGIAMGLCALVTIWCTGMIYASLTTIRAWHQPIVAPLYVVLGLGSGA
                     LLLNLILASFGRSSYAVSGLAIAALLLGRRAYGWRGRIALRWTLAGFMVLLLAYIGSR
                     FVAEV"
BASE COUNT         1097 a         2220 c         2005 g         1114 t
ORIGIN      
        1 atctcgaagg ccggctcaac tccctcgcgc tcgcccacga cctcgcgagc cgcaacgagc
       61 cgatggagct ccgccagctg ctcgagacgg agcttggtcc cttcgacagc gaagccacgg
      121 gccggatcga cctgcgcggc cccgcggtct ggttttccca gcatgccgcg agcgtgatgg
      181 cgctggtggt gcatgagctc gccaccaatg cggccaagta cggagcactc agcaggcccg
      241 aagggtcgat cggcgtgagc tggcaccttg ccaacgacgg aacatgcgac atctcgtggg
      301 tcgagcaaag cccggtgcca gtatcggtcg tggcccggac gggtttcgga accttgataa
      361 tcacgcgtca gatcccattc gagcttggcg gcagcagcga gatgtcgttc acgccgcgtg
      421 gccttgtcgc ccgattgcgt attccggcgg cgagcattgc cctgccgcct cggaccagca
      481 ccggccggcc tctccacgcg agcgcggcaa cagccgatgc cacggcgcag ctcctcgccg
      541 gtcgcacggt tctcgtggtc gaggacaacc tcatcgtcgc gctgcgcttc gagcaggccc
      601 tgaagcacct cggcgccgat cggattgtga tcgcgggcac tctcgagcag gcgatgacgc
      661 tggcgatgtc ggacagcgtc agcctcgtcg tactcgacat caatctcaga gggacgcggt
      721 cctatgctgt cgctgacacc ttgatggagc gcgacgtccc gttcttcttt gcgaccggct
      781 acgggatcga tgcagatctg ccggagcgtt tcaagcacat tccgatcgtc ccgaagccat
      841 ccaccgaggc agcgctgctg cgcacgatca catcggtcct cggtgagctt cgtccggcag
      901 ggtgaggacg ccagcgttgc cgtctgtgcc tgtttgtgcg ccttgctgat ttccaggtca
      961 gtctgatgta atcttggtgc ctgtcatcgc ggcaggtccg ctcggacttg cgccggccgg
     1021 cacggactgc agtcccgggc gtggtcactc gagggtgggg aagagcatgg caagcacagc
     1081 gaaccggatc agcgtcgcgc ttgtcgacga cgaggcggca ttcctgttcg aactttcaca
     1141 gcgcctgatg gaccatggtc taacagtcag gggctaccgg gacgctgcaa ctcttctgtc
     1201 cgacatgcaa aaaggaacga cctacgattg tatcgttgcc gacgtacgca tgccgggtct
     1261 gtcgggcctc gaactgcagc agcgcctgaa cgaaggctcg ctcgaggtcc cgctcatcct
     1321 attcacgggc tatggcgacg tcgatatcgc ggtctcggcc atgaaggcgg gagcctggga
     1381 cttcatcggc aagccgatcg ctgtcgagca tttgtgctcg agcgtcacgt ctgcggtcat
     1441 cgatgcacgc cagcgggccg cggaacgccg ggagcatgcc gccatcgcgg cccgtgtcgc
     1501 cgagctcagt gaacgtcatc gccaggttct cgacctgatg gtgcaaggcc tgacgagcaa
     1561 gcagatcgcc tccacgctca agatcaatca ccgcaccgtc gaaaactacc gcgcatttgt
     1621 gatggaccga attggcgttg gcaatatcgc gcagctggta cgcgtcatgc tccagatgcg
     1681 ctttgcccag aacactgtca gcgctgggca gaagcagccg agcgggacag gctcggaacg
     1741 ggcttaggac ggtcgtccgg tatccgtgcg gagccgcggc aacgcagcac acgccacgcg
     1801 gagcatggac cgagacgcgc gccatcttgg ccgagctacc ggccacgtcg accagcagcc
     1861 gtcgatcagg accagctggg cggcttcggg ccctggctgc ccggtgcgcc cggagcggac
     1921 cttgtgcagc tggcggagag agagggattc gaacccccga tagagttgcc cctatacacg
     1981 cgttccaggc gtgcgcctta aaccactcgg ccatctctcc ggagtgccgg cgagtagcat
     2041 ggctctcccc gaggcgcaac acggcgcggg cgggcatcgt tgctcaggcc ggcgatggtg
     2101 ccggggaaca ccccagcctg cgcgggaatg aagcgtgcat ctcggccggt gcccgcgcaa
     2161 atcgatgctc ccgcgacgcg acgccgcgac agcatcagaa gacttggcgc ggccttggcg
     2221 atgtggaacg gttcgcgtcc aaccatgctc cgggccgggg tcctcccgcc cactgacgac
     2281 cgggccctct ccatgaccgc accgcgtgcg catgccggcg agccgcacat tgcgctctcc
     2341 aagccttcgg cagacgaaat caagaccacc acctgcttca tgtgcgcctg ccgctgcggc
     2401 atccgcgtgc acctgaagga cggcaaggtc cgctacatcg acggcaaccg cgatcacccc
     2461 gtcaacaagg gcgtgatctg cggcaagggt tcggccggca tcatgcagca cacctcgccg
     2521 gctcggctgc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnagag ccaggcgttg accggctggt tcgcgcagca gtacggcacg
     2701 ccaaatttcg cggcgcatgg cggcttctgc tcggtcaata tggcggcggc cggcctctat
     2761 acgtttggtg gcgccttttg ggagttcggc gatccggact gggaccggac ggaatacttc
     2821 atgctcttcg gcgtcgccga ggatcacgcc tcgaacccga tcaagacagc gctcggaaag
     2881 ctaaagaagc ggggcgtcaa gttcgtcgcc gtcaacccgg tgcgcaccgg ctacaacgcc
     2941 atcgcggatg aatggatcgg catcgagccc gggaaggacg gcctcttcgt cgcgggactc
     3001 attcatgagc tcttgcgcac gcagaagatc gacctccctt cgctcgtccg ctacaccaac
     3061 gctccctggc tcgtgatcga tgcgccgggc tccgtggacg acggactctt tgcccgcggc
     3121 gagaacggga agcccctcgc gcacgacaag tcgagcggcg caatcgtgga tgcgagccgc
     3181 gtcgacatcg caccgagcct cgccggcacg ttcaagctcg ccgatggccg cagtgcgagg
     3241 cccgcgttcg aactcatcgc gcggcgtttc gcggcgcccg agaccgcgcc ggaggcgatc
     3301 gagcaggagc tcggcattcc tgctttgacc atccgacgca tcgccgccga gatcgcgcac
     3361 gctgccttcg agaaggaggt cgtcctcgac cagccgtgga ccgactgggc cggccggcgt
     3421 catgaaaaga tgatcggtcg tccggtctcg ttccacgcca tgcgcggcat ctccgctcac
     3481 tcgaacggct tccagacctg ccgcctcatc cacctcatgc agatcctgct cggctccata
     3541 gattgcccgg gcggcttccg ctacaaggcg ccgttcccgc gcccgacccc gccacccaat
     3601 cgtccggccg gcaagcatgg cactgtcaag ccgatgacgc cgctcgccgg cgcgccgctc
     3661 ggcttcccgc aagggccgga ggacctgctc ctcgatcccg acggccggcc gatgcgcatc
     3721 gacaaggcct actcgtggga cgcgccgatc gccgcccatg gcctcatgca catggtgatc
     3781 accaacgccg cgaaccacga cccatacccg atcgacgtcc tgttcatgta catggccaac
     3841 atgagctgga actcgtcgat gaacatcccg gcgatgctca aacatctgac cgaaaaggac
     3901 gccgcgtccg gcgagtacaa gatcccgaag atcatctact ccgacgccta cttctcggag
     3961 atgatcccct acgccgacct catccttccg gacacgacct atctcgagcg gtgggactgc
     4021 atctccatgc tcgaccggcc gatctcggag ccggacggcc cggcggatgc gattcgacac
     4081 ccggtcgtcg agcccgaccg cgacgtgcgc gcgttccagt ccgtcatcat cgacctcggc
     4141 gcccgcctga agttgccggg cttcgtcaag gacgacggct cgcccaaatt cccgggcggc
     4201 tatgcggact acatcgtcaa tcacgaacgt ggaccgggca tcggaccgct cgcaggctac
     4261 cgaggtgccg acggcaaggc ctacggcaaa ggcgcgccca atccgaagca gctcgagcag
     4321 tacatcgcga acggctcttt ctatcagtac cacctcgcac ccgagcaacg ctacttcaag
     4381 cacgccaaca aggcctatct cgactgggca aaggatgtgg gcttcatcgg ctcggcagac
     4441 ccgatcgtct tccagctcta tctcgagccc ttgcagaaat tccgcctcgc ggctctcgga
     4501 catgggacga tcaaggtgcc cgagacgcac cgcgtgagga tagagcggca cttcgatccg
     4561 atcccgttct ggtatccgac atttggcgag gcaccggcgg caagcggggc acgctatccc
     4621 ttccacgcca tcacgcagcg gccgatgcac atgtaccatt cgtggggctc gcagaacgcg
     4681 tggctccgcc agatcaccac cgcgaaccgg ctcttcatga gcgcgtcgga ggctctgaaa
     4741 ctcggcatcc gcgatgacga ctgggtctgg ctggcgagcg agaccgggcg catcaaatgc
     4801 caggtgaaga ccatgcaggg tctgaatccc ggtacggtct ggacctggaa cgccatcggc
     4861 aagcgagcgg gcgcctgggg cctcgcccct gatgctccgg aggcaacgca cggcttcctg
     4921 ctcaaccatc tgatcaccga gctgcttccc gagcagccgg gtggctaccg cttctcgaac
     4981 agcgatccca tcaccggtca ggccgcctgg tacgatctcc gcgtctcgat caccaaggcg
     5041 tcaccgggcg aagccgcgca aagcgagccg cgcttcgagc gcctcgccct cgccgttccg
     5101 accggaggtg cccgatgacc tcgctccccg ccccctcggc caagaagctc ggcctggtca
     5161 tcgacctcga tacctgcgtc ggctgccagg cctgcgcgac gagttgcaag gaatggaacg
     5221 ccgaaggata tccggcgccc ttgtcggacg aggacgccta tggtgccgat ccgcatggca
     5281 cctggctcaa tcgcgtgcat agctacgacg tcggcgcagg cgccgagagc cgcaccgtgc
     5341 acttcccgcg ctcgtgcctc cactgcgaaa cgccggcctg cgtcacggtc tgcccgacgg
     5401 gcgcctccta caagcgcgcc gaggacggca tcgtgctggt gaaccccgac acctgcatcg
     5461 gctgcaagct gtgctcgtgg gcgtgtgcct atggcgcgcg cgagtacgac tacacgcacg
     5521 gcgtcatgaa gaaatgcacg ctctgcgtcg accgcatcta taacgagacc ttcgagcctg
     5581 aggatcgcat cccagcctgc gtgcgtgcct gcccgaccgg cgcccgccac tttggtgatc
     5641 tcggcgatcg caacagcgag gtgtcgaagc tcgttgccga gcgcgccggc ttcgatctgc
     5701 tgccgcagtc gggctacaag ccggtcaaca agtacctgcc gccgcgtccg cggcgcgacg
     5761 ccacgactac ggcaggctct ggcccgtcgg cgaacgctgc ggcaactgga acgcccacgg
     5821 gctcggttgc cgacaggctc ttcgcctggg cagaccgcat gctctcccgc tgatcagaga
     5881 cggttcaccc gatgcatccc gcctactccg tcatcgcatt caccacggcc tcgggcgccg
     5941 gctatgggct gctcgtctgg ctcgccgtct gggggacctt tgcggacctg cctcagggct
     6001 ggcgcgcggc gctcgtcgcg tccgggctgg cgctcggcct cgtctctgca gggctgttgt
     6061 cgtcgaccgc gcatctcggt cggcccgagc gggcttggcg cgcattctcg cagtggcgta
     6121 cctcgtggct gtcgcgggaa ggcgtcgcgg cgatcgtcac ctttgtcccc gccgggctct
     6181 tcgcgttgaa gtggctgatc gacggccgca taggcagcac gaccgcggca atcgggatcg
     6241 ccatgggatt gtgcgcactt gtcactatct ggtgcacggg catgatctat gcctcgctca
     6301 cgaccatccg cgcctggcat cagccgatcg tcgccccgct ttatgtcgtg ctgggcctag
     6361 gctcgggcgc attgctgctc aatctcatac tcgcgtcctt cggccgctcg agctacgcgg
     6421 tctccggatt ggcgatcgcc gccctcctgc tcggtcgtcg cgcatatggc tggcgaggcc
     6481 gcatcgcgct gcgctggacc ctggccggtt tcatggtctt gctgctcgcc tatatcggga
     6541 gccgctttgt tgccgaggta tt
//
LOCUS       DAORNH010000266         6562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8707_length_6562_cov_2.586138, whole genome shotgun sequence.
ACCESSION   DAORNH010000266 DAORNH010000000
VERSION     DAORNH010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8707_length_6562_cov_2.586138"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    220..402
                     /estimated_length=183
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    613..622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<623..1456)
                     /locus_tag="PK264_20365"
     CDS             complement(<623..1456)
                     /locus_tag="PK264_20365"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HRD78258.1"
                     /translation="MATAQQMSSATSAPNWPFEVRKLHPAFGAEIIGLPLADAVKDEN
                     FPKVYECFLEHKLILFRGVDLPQATQVAFAKRFGPVQVHVMNQYHGRGHPEIYYLSNL
                     DDKGQPNGKHPDKGTLYWHTDGSWRDVGTTATMMYSEVVPEAGGHTEFADTCAAYDAL
                     SPEWKLRIADLKAVHNLDFSRTRRHGHEPMTAEQKAKVPPVAHPIARVHPETGRRAIY
                     LGDHAESIAGMDYAAGRALIEELNVLATPQHVVYSHAWKPGECIVWDNRSTLHRATGF
                     DT"
     gene            complement(1485..2810)
                     /locus_tag="PK264_20370"
     CDS             complement(1485..2810)
                     /locus_tag="PK264_20370"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HRD78259.1"
                     /translation="MEISPLWMFPALIAAILTGFPVALTMMGLAVIFGLSTFGHTGLL
                     YQFIQKIEEVATAQVLAAVPLFIFMGAMFEQTGIASRLFDAIQMWIKRLPGGLAVGTV
                     IMCVIFAATSGVVGATETVVGLLAIPAMLRYNYNKGLISGTICAGGSLGTIIPPSILA
                     VVIGPTAQADVGKVLLGMVIPGLVLAGSYIIYIVLRCMLRPEDGARIAAREDDPTLGQ
                     KLAITAQVLIPPIVVIIAVIGSMMVGIATPTEAAATGALGTLALAVVYRKLNWAVLVD
                     ATMRTVRITAMILTIVACGQMFAAVFVGSGGLQILQDFLLSFGVGKWGLVGLVMLIVF
                     IAGFMLEPLVIILMVVPITAPIVVGQGFDKEWFCVLFLVMLQTGYLTPPMAPSIFYLR
                     GIAPPEITLRHMYTGIWPFIGLQLMVVALIMAFPGLVTWLPSVAQTGFK"
     gene            complement(2866..3393)
                     /locus_tag="PK264_20375"
     CDS             complement(2866..3393)
                     /locus_tag="PK264_20375"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HRD78260.1"
                     /translation="MLVRSIDALSRTLGGVAAVLVLVLVALMLYDVLLRYAFNAPTSW
                     GNDMNTYLMGASFLLSIAYAMSTDSHVRVDLLYNEKTRGRLAWVDLIGLTLLTFPTLV
                     WLSWGLWEHFFESLKSGERSGSGGWNPVVWPFKLVMFVGFLFFAIQVGAEIIKRLYAV
                     SGAPLDAAKADEHAM"
     gene            complement(3520..4563)
                     /gene="dctP"
                     /locus_tag="PK264_20380"
     CDS             complement(3520..4563)
                     /gene="dctP"
                     /locus_tag="PK264_20380"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HRD78261.1"
                     /translation="MRPSVTKVAQLLAAGCLLAGGSAPADAQVRMATSWPGGPHLELF
                     AKGFATTAESLTGGKVKFQVFPAGTIGSPLKMAETVQKKVAPAAHTWPGYDWGIDKAA
                     AIFGGYVGSPPTEALLHWVYSAGGLQMWKDWRAEKFGIVGFPCGAHSDEIHMHSRKPV
                     RTLADLKGLKLRTSGAWAEIATTLGASTVILAGAEVYPALERGVVDAIEWATPGINLG
                     LGFHKVAKYVILPGVHQPGAVLECLFDKATWDGFDAVTKTQIEAAAKHATLSAWMAIN
                     MLDADALGKLKEAGAEIIRVDQAYIDGVAKATRAWEDKYIAEGGWFKKVVEHQRAFME
                     KWKPSSQYRSEFR"
     gene            4759..5331
                     /gene="mobA"
                     /locus_tag="PK264_20385"
                     /pseudo
     CDS             4759..5331
                     /gene="mobA"
                     /locus_tag="PK264_20385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
     gene            5368..5904
                     /gene="mobB"
                     /locus_tag="PK264_20390"
     CDS             5368..5904
                     /gene="mobB"
                     /locus_tag="PK264_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HRD78262.1"
                     /translation="MFDAAPPRPPVIGIAGWKNSGKTGLVERLVVELARRGLRVATVK
                     HAHHTFDVDPGSTDSARHRRAGAIQVAIVSERRWAIMRELSQDAEPPLEAVIARLDRA
                     DIVIVEGYKRAAIPKIEARRAAGARGEPLSASDANVIAIAADHAVSETTLPAFHLDEI
                     GRIADFLATRFRLPAQPR"
     gene            complement(5892..>6562)
                     /locus_tag="PK264_20395"
     CDS             complement(5892..>6562)
                     /locus_tag="PK264_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78263.1"
                     /translation="FRELPRACREPVVALAPPVLIPPSMAVATADPADASLTGEVATR
                     RAPIELQAEVIGSGAAVSDAGSVRTISPGGLAAVTALAPPEPDMANEAKSEAAPPGRI
                     AMRFALAPPEPDMASELGLPAIPVVMAVVVAVAPANPTATNPTTAPCVEVSAMELPLL
                     HAVPAGEPVQPREPARSSDRASAMRSTASVVELRNRERGRRKGRPVYRLAVDFPIVEL
                     AHRG"
BASE COUNT         1098 a         2121 c         2118 g         1032 t
ORIGIN      
        1 gccgatgtcg ccgccgcctt acgccaacgg tgtggatcat ggcgccatgc atgcacgccc
       61 tatgggtgct ggtgcgccga ccgcgggccc gccgatgacg cgcgagccgg ctcacgcaac
      121 gcatgcagcg cacatgggca cagcgcttcc cgagcccacc gatcggtacc ggatggggcg
      181 gctgctgacg cgcattttca cggtgctcgg tgtgcttggn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcgcgct gatcgacagc
      421 gccaacgccg cgcaggacct cgtcgctatc gagcgtgcga agtctgggca ctgaggcggc
      481 gcccggcctt gaagacgacg atgccgtgat gcgcgagcat cacggcattt tctctagccg
      541 gtaaggggcg gctgcggctc agaacacctg cgtgccgagc atcgtgcagc ggcgcatgac
      601 gcgcttgtgc ttnnnnnnnn nncgtgtcga agccggtcgc ccggtgcagc gtcgagcggt
      661 tgtcccaaac gatgcactcg ccgggcttcc aagcatgcga gtagacgaca tgctgcggcg
      721 tcgcgagcac gttgagctcc tcgatgagcg cgcggccggc cgcatagtcc attccggcga
      781 tcgattcggc atggtcgcca aggtagatcg cccgccgccc ggtctcggga tggacgcgcg
      841 cgatcggatg cgcgacgggc ggcaccttcg ccttctgctc ggctgtcatc ggctcgtggc
      901 cgtgccggcg cgtacgcgag aagtcgaggt tgtgaacggc tttgagatct gcgatccgca
      961 atttccactc gggcgagagc gcatcatagg cggcgcaggt atccgcgaac tcggtgtgcc
     1021 cgccggcctc gggcacgacc tcggagtaca tcatcgtcgc cgttgtgccg acgtcgcgcc
     1081 aggagccgtc ggtatgccag tagagtgtgc ccttgtccgg atgtttgccg ttcggctggc
     1141 ctttgtcatc gaggttcgag aggtagtaga tctctgggtg cccgcgccca tggtactggt
     1201 tcatcacgtg cacctgaacc ggcccgaagc gcttggcgaa cgcgacctgc gtcgcctgcg
     1261 ggagatcgac gccacggaac aggatcagct tgtgctcgag gaagcactcg tagaccttcg
     1321 ggaaattctc gtccttcacc gcgtcagcaa gcgggaggcc gatgatctcg gcgccgaatg
     1381 cgggatggag ctttctcacc tcgaacggcc agttgggcgc gctcgtcgcc gacgacatct
     1441 gctgtgcggt agccatgaga gcctcctgtt tcaggtggag ccgatcattt gaagccggtc
     1501 tgcgcgacgc tcggcagcca tgtaacgagc cccgggaacg ccatgataag ggcaacgacc
     1561 atcagctgca ggccgatgaa cggccagatg ccggtgtaca tgtgtcggag cgtgatctcc
     1621 ggaggagcaa tgccgcgcag atagaagatc gagggcgcca tgggcggcgt caggtagccc
     1681 gtctggagca tgacgaggaa gaggacgcag aaccactcct tgtcgaagcc ctggccgacg
     1741 acgatcggcg cggtgatcgg aaccaccatc aggatgatga cgagtggctc cagcatgaag
     1801 cccgcgatga agacgatcag catcacgagg ccgacgaggc cccatttgcc gacgccgaag
     1861 gacaggagga aatcctgcag gatctgcagc ccgcccgagc cgacgaacac ggccgcgaac
     1921 atctgtccgc aagcgacgat cgtcaggatc atcgccgtga tgcgcacggt acgcatcgtg
     1981 gcatccacca gaacggccca gttgagcttg cggtagacga cagccagcgc cagcgttccg
     2041 agcgcgccgg tcgcagcagc ctcggtcggc gtcgcgatgc caaccatcat cgagccgatg
     2101 accgcgatga tgacgacgat cggcgggatc agcacctgcg cggtgatcgc gagcttctgc
     2161 ccgagcgtcg gatcatcctc ccgcgccgca atgcgggcgc cgtcctcggg tcggagcatg
     2221 caacggagca cgatgtagat gatgtagctc ccggcaagca ccagacccgg gatgaccatg
     2281 ccgagcagca ccttgccgac gtcggcctgg gctgtcggac cgatgacgac ggcgaggatc
     2341 gagggcggaa tgatggtgcc gagggagccg ccggcgcaga tcgtgcccga gatgaggccc
     2401 ttgttgtagt tgtagcggag catcgcaggg atcgcgagca ggccgaccac cgtctcggtt
     2461 gcgccgacga cgccgctcgt cgccgcgaag atcacgcaca tgatgacggt gccgacggcg
     2521 agcccgccgg gcagccgctt gatccacatc tgaatggcgt cgaagaggcg gctcgcaatg
     2581 cccgtctgct cgaacatcgc gcccatgaag atgaagagcg gcacggccgc gagcacctgc
     2641 gcggtagcta cctcctcgat cttctggatg aactggtaga ggaggcccgt atggccgaat
     2701 gtgctcagcc cgaagatcac cgcgaggccc atcatggtca gcgcaaccgg aaacccggtc
     2761 agaatggcgg cgatcagtgc ggggaacatc cagagaggcg agatttccat tgtcgtgtcc
     2821 ttgaggggcg ctggcgggct gggaacgcgt ttcaccgcgg ggcggtcaca tcgcgtgctc
     2881 atcagccttc gcggcatcga gcggggcgcc tgaaacggca tacaagcgct tgatgatctc
     2941 ggcgccgacc tggatcgcga agaataaaaa gccgacgaac atcacgagct tgaacggcca
     3001 cacgaccgga ttccaaccgc ccgagcccga acgctcaccg gatttcaagg actcgaagaa
     3061 gtgctcccac aggccccagg acagccagac gagcgttggg aatgtcagta gcgtgaggcc
     3121 aatgaggtcg acccaggcga gccgaccgcg cgttttctcg ttgtagagaa ggtcgacccg
     3181 cacgtggctg tccgtcgaca tcgcataggc aatcgagagc aggaacgagg cgcccatgag
     3241 gtaggtgttc atgtcgttgc cccagctggt cggggcattg aacgcatagc gcagcagcac
     3301 gtcgtagagc atcagcgcga cgagcacgag caccaggact gccgcgacgc cgccaagcgt
     3361 gcggctcagc gcatcgatgc ttctgacgag cactctcatg tcttcccccg aacccgcagg
     3421 cgccgcagcg ctgtctccgg ccccatcgcc ggccgcttca cacgcgccgc ggatgtggcg
     3481 cctcgctctc tattttctga ctggaacgtc gcgaccggat cagcggaact cggagcggta
     3541 ctggctcgag ggcttccact tctccatgaa ggcgcgctga tgctcgacta cctttttgaa
     3601 ccagccgcct tccgcaatgt acttgtcctc ccaggcgcgg gtcgccttgg cgacgccgtc
     3661 gatgtaggcc tgatcgacgc ggatgatctc ggcgccggct tccttgagct tgccgagcgc
     3721 gtcggcgtcc agcatgttga tcgccatcca ggcggaaagc gtcgcatgct tcgccgcggc
     3781 ctcgatctgc gtcttggtaa cggcgtcgaa gccatcccag gtcgccttgt cgaataggca
     3841 ctcgagcacc gcacccggct ggtgcacgcc gggcaggatc acgtacttcg cgaccttgtg
     3901 gaagccgagg cctagattga tgccgggcgt cgcccactcg atggcgtcga cgacgccgcg
     3961 ctcgagcgcc ggatagacct cggcgccggc gaggatgacg gtcgaggcgc cgagcgtcgt
     4021 tgcgatctcg gcccaggcgc cggaggtgcg caacttcagg cccttgaggt ccgcgagcgt
     4081 gcgcacgggc ttcctggagt gcatgtggat ctcatcggaa tgcgcgccgc acgggaatcc
     4141 gacgatgccg aacttctcgg cgcgccagtc cttccacatc tgcaggccgc cggccgagta
     4201 gacccaatgg agcagtgctt ccgtcggcgg cgagccgaca tagccgccga agatcgcggc
     4261 cgccttgtcg atgccccagt cgtagccggg ccaggtgtgg gcggcaggcg ccaccttctt
     4321 ctgcaccgtc tcggccatct tcagcgggct gccgatcgtg ccggccggga agacctggaa
     4381 cttgaccttg ccgcccgtga gcgattccgc ggtcgtcgcg aagcccttcg cgaagagctc
     4441 gaggtgcggt ccgcccggcc aggacgtcgc catccgaacc tgggcatcgg ccggcgcaga
     4501 accacccgcc agcaggcatc cggccgccag caattgagcg accttcgtta cactcggtct
     4561 catgcggact tcctcccatc gtttggtctt ggtgtccgag ccaccgccga cttcgggtcg
     4621 gtcagccagc atgtgccgac aggctgaggc acctaatata ccagactgcc gcttggcggc
     4681 ccgtctgaca agcgctaagc gtcgaaacgg ctcgcaatgg caaatcgcat gcactcctac
     4741 gagcaggaca cacggacaat gcgcgtcgca gttgttctcg ccggcggact gtcgcgtcgc
     4801 atgggcggcg gcgacaaggg cctcctcgac gttgccggca ccagcatgat cggccgcgtg
     4861 atccgtctga cgcgcccgca ggtcgcccag ctcgccatca acgccaacgg cgatcccatg
     4921 cgctttgcct cgctcgggcc tccggtgatc gccgacacga tcgcggggca tccgggccct
     4981 ctcgcggggg ttctcgccgg catgcgatgg gcagccgtcg cggcacctgg tgcaacgcac
     5041 ctgctttccg tgtcgtccga tgcgccgctg cttccgggcg atctcgccga gcggcttgcc
     5101 gcggcattgg tgtcccgccc aacatcgatc gccatcgcgc gctcgcgcgg ggaattgcat
     5161 cccgtgatcg ggctctggcc cgtcgccctc gccgatgacc tcgaggcctg gctcaagggg
     5221 gggggccgca aggtcctcgc ctggagcgat cggcacggca cggtccccgt cgatttcgcg
     5281 gaacattggc tcggcggcgt gccggtcgat ccgttcttca atgccaacac gccggaggag
     5341 atcggcgagg cgcgagcgct cgcgcgcatc ttcgatgccg cgccgccgag gccgccggtg
     5401 atcggcatcg cgggctggaa gaactccggc aagaccgggc tcgtcgaacg cctcgtcgtc
     5461 gaactcgcac gccgtggcct ccgggtggca accgtcaagc atgcccatca cacgttcgac
     5521 gtcgaccctg gctcgaccga cagcgcccgc catcgccgtg cgggcgccat ccaggtggcg
     5581 atcgtctcgg agcgccgctg ggcgatcatg cgcgaactca gtcaggacgc cgagccgccg
     5641 ctcgaggccg tcatcgcgcg gcttgatagg gccgacatcg tcatcgtcga gggctacaag
     5701 cgcgcggcca tcccgaagat cgaggcgcgt cgcgcggcag gggcacgggg agagccactt
     5761 tccgcaagcg atgccaacgt catcgccatc gccgccgacc acgccgtgag cgagactact
     5821 ctgccggcgt tccatctcga cgagatcggc cggatcgcgg atttcctggc gacacgcttc
     5881 cgcctcccag ctcagccgcg gtgagcgagc tccacgatcg gaaagtcgac cgcgagccga
     5941 tagacggggc gccccttgcg gcgcccacgc tcgcggttcc ggagctcgac cacactggcc
     6001 gtggagcgca tcgccgacgc tcgatcgctg ctgcgtgccg gctcgcgcgg ctgaactggc
     6061 tcgcctgccg ggacggcatg aagcaacggg agctccatgg ccgaaacctc gacgcatggc
     6121 gccgtcgtcg gattggtcgc ggtcggattg gcaggcgcga ctgcgaccac cacagccatc
     6181 acgaccggta ttgccggcag cccgagttcg ctcgccatat cgggctcagg cggcgccagc
     6241 gcaaatcgca tggcaattct gccgggcgga gcggcctcgc tcttcgcctc attcgccatg
     6301 tcgggctcgg gcggcgccag cgccgttacg gcagcaagcc cgccgggcga gatcgtccga
     6361 acggacccag cgtcgctgac agccgcgccc gatccaatga cctcggcctg aagctcgatc
     6421 ggcgctcgcc gtgtggcgac ttcgcccgtg agactggcat ccgcaggatc ggccgtagcg
     6481 acggccatgg acggcggaat gagcaccggc ggcgcgagcg cgaccacagg ctcgcgacag
     6541 gcgcgcggca gctcgcggaa cg
//
LOCUS       DAORNH010000267         6538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8757_length_6538_cov_2.825852, whole genome shotgun sequence.
ACCESSION   DAORNH010000267 DAORNH010000000
VERSION     DAORNH010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8757_length_6538_cov_2.825852"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1942
                     /locus_tag="PK264_20400"
     CDS             398..1942
                     /locus_tag="PK264_20400"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD78264.1"
                     /translation="MDTFTGHEVGGGQEAKRAAGRASAHFDVLIVGAGISGIGGAYHL
                     QKQCPGTSFVVLEAEESFGGTWWTHRYPGIRSDSDLHTFGYRFKPWTGPPIATAEEIL
                     AYLGEVIAENDIARHIRYRHRIERASWSSETCRWTVEAVRLETGERLTFSAGFLWMCQ
                     GYYRHRDGYTPEWPGMADFKGQIIHPMSWPEDLDYTDKTVVVIGSGATAATVVPAMAE
                     KTRHITMLQRSPTYFRTGRNAIPIAEELRQLQVDEAWVHEITRKKILYEQSAFTKRTF
                     SEPDKVTAELLGQVRAFLGPDYDIATHFTPRYRPWRQRLAFIPDGDLFCGIRDGKASV
                     VTDEIDRFVPEGIRVKSGRVLPADIIVTATGFHMSALGDIAFTIDGKPLDFANTVTYR
                     GMMFTGIPNMAWVFGYFRASWTLRSDLVADFVCRLLNHMKAKGVKSVVPALRPQESNM
                     PILPWIDEENFNPGYLTRSMAILPKRGDKAEWAHTQDYWAEKDEFPAVDLDDVVFVYG
                     GNGEVR"
     gene            2101..4203
                     /locus_tag="PK264_20405"
     CDS             2101..4203
                     /locus_tag="PK264_20405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HRD78265.1"
                     /translation="MSVSEPKAKRSVGSGVISRRALLLGAAGASVASMANWGAIRART
                     LIPRADLFGHPKFRAVDISPDGTMLSWLAPSTFFGVSAIWVAPRERLEAARQVTARTS
                     SIDSYWWSDGSAFLYFYSDREGDEDQHLFAVEVATGTTRDLTPFDKIHALALGDTKDD
                     DIGVAINNRDPKWHDIWRINARTGERSLIFANTEQFGSIVLDGALRPRLGSRDLPANK
                     GTELIRLDDGARTPFLEIEAEDAATFNVLGFNRRGDTLFALSAIGRDRMALVAYDWAS
                     RTEQLLGAHPKADVVNANVNWQTGDPDLITATHLRTEWIGLTDRARKLLELVDRLPTG
                     EITRSVGRSDDDRYWVAERTGPTEPGHYYLIDTISGAATRLFEANPKLKTYRLARVHP
                     EVIRARDGLELVSYLTLPIAASERVRGRPARPQPMVLLVHGGPAVRDSYEFDTKVQWL
                     ADRGYAVLQVNFRGSTGFGKAFVNAGDGQWGRQMHDDLIDAVEWAIAQRIASRDRIAI
                     MGASYGGYAVLVGLTFTPEVFRCGVCINGVSNLETDLGAIPAYWSGAFEQLARQKGDP
                     RTEEGRKRLRDASPIHRVAAIRRPLLISNGANDPRVNKAQTQQMVDAMKARSMPVIHA
                     LYPHGGHTFFDDNAYPAVAEMFLAHHLGGAVEPFGTDIETSSLQVTEGAEQLPGLKDE
                     LARLCTKSIATALCPGAK"
     gene            4427..>4974
                     /locus_tag="PK264_20410"
     CDS             4427..>4974
                     /locus_tag="PK264_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD78266.1"
                     /translation="MTRPIHLNLFIYARGHHEAAWRHPKAPARSLMDIDHVVECARLA
                     EAAHMDSIFLADVLNLPSDADRTARIWLEPLTALSAVAMATRSIGLIGTASTSHYEPY
                     NVARLFNSLDHISRGRAAWNIVTSFSIEGSRNFDSSGRRAHTDRYERGDEFVDVVKKL
                     WDSWNDDAIADDRAAGIYYRAER"
     assembly_gap    4975..5005
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5006..5750
                     /locus_tag="PK264_20415"
     CDS             <5006..5750
                     /locus_tag="PK264_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD78267.1"
                     /translation="GAHYDVAGPLNLPRSPQGWPVLVQAGSSDTGKAFAAKHAEAVFT
                     AHMTKATAREFYADLKARVATIGRPADQCLVLPGISMVIAGTEAEAKRLERDLNEMSD
                     IATGLSRLSQRFDGHDFSHIPLDRVLTARDFPDPAVNQSSRGRTELIVGAVERETLTM
                     RQLLGKLAGARGHFVIAGTPEQIADAIVDWVDDGAADGFNVMPLLLPEMLAVFATEVV
                     PILKRRGRFRSGYSGTTLREHYGLRRPSA"
     gene            complement(5775..6083)
                     /locus_tag="PK264_20420"
     CDS             complement(5775..6083)
                     /locus_tag="PK264_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HRD78268.1"
                     /translation="MSKIDPKTDIELQAAAYRRLVEHLRTRTDVQNIDLMNLAGFCRN
                     CLANWYQDAAGVKGIKLSKEQAREVVYGMPYDEWRARHQTEAGAEKKAAFEKARPKEH
                     "
BASE COUNT         1163 a         2104 c         2055 g         1185 t
ORIGIN      
        1 ccccggtccc gatcacgccc agcacgcgtt accgtcgtcg atcgaccgtt gggtgcgacg
       61 accatagacg cgcccgccga gcccggggac aacatcgtcg cttcgcgatt gggcttccgc
      121 ccaaacccga tcgggcctct ggccggcccg aacccggccc attttgaaac ccaaaaaaat
      181 gggggaggcc cggaggatct cctccgggcg gagtgcgtcg tgctggaagc gcgccactag
      241 gagagtgcgg cagccccgct cgcttgcacg agtgatgtcg gcccgtcgat cgaactgtcc
      301 gccgcgcaga cgtgccacgc gctacccaag ggttgattta tcgcctcttc cgcctgacac
      361 tcgcccgagc aatccgacag tctgaccagg gtgggccatg gacacgttca cagggcacga
      421 agtgggcggg gggcaggagg cgaagagggc cgccggccgc gccagcgcgc atttcgacgt
      481 gctgatcgtc ggggcaggca tctccggcat cggcggcgcc taccacctcc agaagcagtg
      541 ccccgggacg agcttcgtcg tgctcgaggc cgaggaaagc ttcggcggca cctggtggac
      601 ccaccgctac cccggcatcc gctccgacag cgacctgcac accttcggct accgcttcaa
      661 gccctggacc ggaccgccga tcgcgacggc ggaggagatc ctcgcctacc tcggcgaggt
      721 gatcgccgag aacgacatcg cgcgccacat caggtatcgc cacaggatcg agcgcgcatc
      781 ctggtcgagc gagacgtgtc ggtggaccgt tgaggcggtg cgactcgaga ccggcgagcg
      841 gctcactttc tcggccgggt tcctctggat gtgccagggc tactaccgtc accgcgatgg
      901 ttacacgccc gaatggcccg gcatggctga cttcaagggc cagatcatcc atccgatgag
      961 ctggccggag gatctcgatt acaccgacaa gaccgtcgtc gtgatcggtt cgggcgcgac
     1021 ggcggcgacc gtcgtgcctg cgatggccga aaagacgcgg cacatcacca tgctgcagcg
     1081 ctcgccgacc tacttccgca ccgggcgaaa cgcgatcccg atcgcggagg agctgcgcca
     1141 gctgcaggtc gacgaggcct gggtgcacga gatcacgcgc aagaagatcc tctacgaaca
     1201 gtcggcgttt acgaagcgca cattcagcga gcccgacaag gtgacagccg agctgctcgg
     1261 ccaggtgcgc gccttcctcg ggcccgacta cgacatcgcc acccacttca cgccgcgcta
     1321 ccggccgtgg cggcagcgac tcgccttcat cccagacggc gacctcttct gcggcatccg
     1381 agacggcaag gcctctgtcg tcaccgacga gatcgatcgc ttcgtgccgg agggcatccg
     1441 cgtaaagtcg ggtcgggtgc tgcccgccga cattatcgtg acggcaaccg gcttccacat
     1501 gagcgcgctc ggtgacatcg catttacgat cgacgggaag ccgctcgact tcgcgaacac
     1561 ggtcacctac cgcggcatga tgttcaccgg catccccaac atggcctggg tgttcggcta
     1621 cttccgcgcg agctggacac tgcgctccga tctcgtcgcc gatttcgtct gccggctgct
     1681 caaccacatg aaagcgaagg gcgtgaagag cgtcgtgccg gcacttcggc cgcaggagtc
     1741 caacatgccg atcctgccct ggatcgacga ggaaaacttc aatccgggct acctcacgcg
     1801 cagcatggcg atcctgccga agcgcggcga caaggcggag tgggcgcata cccaggacta
     1861 ctgggccgag aaggacgagt tccccgcggt cgacctcgac gatgtggtat tcgtttatgg
     1921 cggcaacgga gaggtgcggt aaaaaatacg tggactgtcg atcggttgcg tgtgccacat
     1981 ttgcgactga acggcgggat cgtcgaattg ccgtagtcgc gcctcgacgt cgaaattgat
     2041 ccccggccgc aactttaatt tcactgtcgg ttttcagaca gatttcccgg agcagcaatt
     2101 atgagtgttt ccgagcccaa ggccaagcgc agcgttggga gtggcgtgat ctcgcggcgc
     2161 gccctcctgc ttggtgccgc aggcgcatcc gtggcaagca tggcaaattg gggcgccatt
     2221 cgcgcccgca cgctgatccc tcgcgcagac ttgttcggcc atccgaaatt tcgtgccgtc
     2281 gacatttcac ctgacggcac catgctgtcg tggctggccc cctcaacgtt tttcggtgta
     2341 tccgccatct gggttgcgcc gcgcgagcgg ctggaggccg ctcggcaagt tacggctcgg
     2401 acttcgtcaa tcgacagtta ttggtggtcc gacggtagtg cctttctgta tttctatagt
     2461 gatcgcgaag gcgacgagga tcagcatctt tttgcagtcg aagtggcgac cggaacgacg
     2521 cgagacctta ccccgttcga taagatacat gcccttgcgt taggcgatac gaaagatgac
     2581 gatatcggag tggcgatcaa caatcgcgat ccgaaatggc acgacatctg gcgcatcaat
     2641 gccaggactg gcgagcgcag tttgatcttt gcaaacaccg agcagttcgg ctcgattgtg
     2701 ctcgacgggg ccttgcggcc ccgccttggt tcacgcgatc tgcctgccaa caagggcacg
     2761 gaactcattc gtcttgatga cggcgcccgc acgccgttcc tcgagatcga ggccgaggat
     2821 gcggcgacgt tcaacgtcct cggcttcaat cggagaggtg atacgctctt cgcgctttcg
     2881 gccatcggcc gcgatcgcat ggcccttgtt gcgtacgact gggccagcag aaccgagcag
     2941 ttgcttggcg cacatccgaa ggcagacgtc gtcaatgcca acgtcaattg gcagacaggt
     3001 gaccccgatt taatcacggc gacccatctg cgcaccgagt ggatcgggct gactgataga
     3061 gcacgaaagt tgctcgagtt ggtcgaccgt ttgcccacgg gcgagatcac tcgatcagtc
     3121 gggcggtcgg atgatgatcg ctattgggtc gctgagcgga caggcccgac cgagcccggt
     3181 cactattatc tgatcgatac catctcgggc gcggcaactc ggttgttcga ggccaatccg
     3241 aaactcaaga cctaccgttt ggcacgcgtg cacccagaag tcattcgcgc gcgtgacgga
     3301 ctggaactcg tatcctatct cacgctgccg atcgctgcct ccgagcgggt taggggcagg
     3361 ccggctcgac cgcagccgat ggtgctgctt gtgcatggcg gaccggccgt acgcgactcg
     3421 tatgaattcg acacgaaggt gcagtggctt gcagaccgag gctacgcagt cctgcaggtg
     3481 aattttcgag gatcaacggg gttcggcaaa gccttcgtga atgccggtga tggccagtgg
     3541 ggccgacaga tgcacgacga cctgatcgac gcggtcgaat gggctatcgc tcaacgcatc
     3601 gccagccgcg accgcattgc catcatgggc gcatcctatg gcggctatgc ggtgctggtc
     3661 ggcctcacgt tcacgcccga ggtgttccgc tgcggcgtgt gtatcaatgg cgtttccaac
     3721 ctcgagactg acttaggcgc tattccggcc tattggtcgg gcgccttcga gcaattggcg
     3781 cgtcaaaagg gggatccccg tacggaggag ggacgcaagc gtctccggga tgcctctcca
     3841 attcatcgcg tggcggcgat ccggcggccg ctgctgatca gcaacggcgc caatgacccg
     3901 cgcgtcaaca aagcccagac ccagcaaatg gttgatgcaa tgaaagcgcg ctccatgccg
     3961 gtcattcatg cgctctaccc tcatgggggc catactttct tcgatgacaa cgcatacccg
     4021 gcggtggccg agatgtttct agcgcaccat ctcggcggcg cagtcgagcc cttcggcacc
     4081 gacatcgaga cttcatcgct tcaagtgacc gaaggtgccg agcaattgcc gggcttgaag
     4141 gacgaactcg cgcggctatg caccaaatcc atcgcgacgg cgctttgccc gggcgctaag
     4201 tgatgtcggt ggccgatgca ggttttgacg aacgccagca ggcactgctc tacggtcaac
     4261 agccgcaaga ccctgctccg aagcgcagca gagtttactg gcgtgaagca ccgcgcactt
     4321 aaccattggc cgaccagcgg tgccggcgcc tacggctgac gtgcggcggt ttcccggcac
     4381 acgagggatg ttggtcagtg ccccaggtac gcaccggaga tcgagcatga cgcggccaat
     4441 ccatctgaac ctcttcatct acgctcgcgg gcaccatgaa gccgcgtggc ggcacccgaa
     4501 ggcgccggcc cgatcactca tggacatcga ccatgtggtc gagtgcgcgc gcctcgccga
     4561 ggccgcacac atggattcga tcttcctcgc cgacgtactg aacctgccat cggatgccga
     4621 ccgtaccgcc cgcatctggc tcgagcccct gacggcgctc tccgccgttg caatggcgac
     4681 gcgctcgatc gggctcatcg gcacggcctc tacctcgcac tacgagccct acaacgtcgc
     4741 gcggctcttc aactcgcttg accatatcag ccgcggtcgg gctgcctgga acatcgtgac
     4801 ctccttcagc atcgaaggga gccgcaactt cgacagcagc ggccgtcgcg cccatacaga
     4861 tcgctatgag cgcggcgacg agttcgtcga tgtcgtgaag aagctctggg acagctggaa
     4921 cgacgatgcc atcgccgacg acagggccgc cggcatttac taccgggccg agcgnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnncggcg cccactacga tgtcgccggc cccctcaatc
     5041 tgccgcggag cccgcaaggc tggcccgtgc tcgtgcaggc gggctcgtcc gataccggca
     5101 aggcgttcgc cgccaagcac gccgaggcgg tcttcacggc gcatatgacc aaggcgaccg
     5161 cgcgcgagtt ctacgctgat ctgaaggccc gcgtcgccac aatcggccga ccggcggatc
     5221 agtgcctcgt cctaccaggc atcagcatgg tgatcgccgg taccgaggcc gaggcgaagc
     5281 gcttggagcg tgatctcaac gagatgagcg acatcgcgac cggcctctcg cgtctctcgc
     5341 agcgcttcga cgggcacgac ttctcgcaca ttccgctgga tcgcgtgctc acggcgcgcg
     5401 acttccccga tccggcggtg aaccagagct cgcgcggccg caccgagctc atcgtcgggg
     5461 ccgtcgagcg tgagacgctg accatgcgcc agctgctcgg caagctcgct ggtgcccgcg
     5521 gccatttcgt catcgccggc acgcccgagc agatcgcgga cgccatcgtg gattgggtcg
     5581 acgacggcgc ggccgacggc ttcaacgtga tgccgctgct gctgccggaa atgcttgccg
     5641 tgttcgcgac cgaggtcgtg ccgatcctga agcggcgggg ccgcttccgc agcggctatt
     5701 caggcacgac cctgcgagag cattatgggc tgaggcgccc ttcggcctga acagcgcgcg
     5761 gccgtcgcct ttgctcaatg ctccttcggg cgcgccttct cgaaggcggc cttcttttcg
     5821 gcaccggcct cggtctggtg gcgcgcgcgc cactcgtcgt acggcatgcc gtagaccacc
     5881 tcgcgtgctt gctccttact gagcttgatg cccttcacgc cggcagcatc ctggtaccag
     5941 ttggcgaggc agttccggca gaaacctgca aggttcatga ggtcgatgtt ctgcacatcg
     6001 gtgcgcgtgc gcaggtgctc gacgaggcgg cggtaggcgg ctgcctggag ctcgatatcg
     6061 gtcttggggt cgatcttgct cattgtccgt ccttcacgtt ccatccgtcg atgcgcggaa
     6121 tggaggcttc acatgccgcg accttggcac ccgcctggca acttgtcacg gcccgttccc
     6181 gaggcccgat cgcgacgcga gatcgatgcc gacgcgcgag agcgccttcg atgttcggtc
     6241 ggcttgtccc tctcgctcga ccgatcaaca atcttctgtt gctgcggccg attggcagca
     6301 cgtcaccgag agcgactcgc ggcaaggtgg cattgagaga ttggggaccc gaaggggaat
     6361 ggcatgtcgc tatacgaggc gctcgaggaa ttcgtcggcg cgggtgttct cgcgagcgcg
     6421 gctgcctgcg aggtggaggt gccgatcggc gcggtgagcg gcggcgggcg gctcgtgacg
     6481 ctgctccgtc agcgtggtgc ggcgagctat gtgacgccgc tcatcgacat gctctcgc
//
LOCUS       DAORNH010000268         6482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8842_length_6482_cov_2.661895, whole genome shotgun sequence.
ACCESSION   DAORNH010000268 DAORNH010000000
VERSION     DAORNH010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8842_length_6482_cov_2.661895"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK264_20425"
     CDS             complement(<1..218)
                     /locus_tag="PK264_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78269.1"
                     /translation="MAGRLLSLATRARTKAPMIEAGRCLVTRERGLEGDFRGAKYPRR
                     QVTVLAREDWEAALAELAAVAGAARLPWT"
     gene            complement(224..793)
                     /locus_tag="PK264_20430"
     CDS             complement(224..793)
                     /locus_tag="PK264_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HRD78270.1"
                     /translation="MLLLTLGLALFIAIHLVPTQPQLRASLVGRFGEGGYKGVFSLIS
                     LAGLALVVMAWAKAKHVPLWTPPGWGRHAAYTLMLPVFPLLVMAYLKGPIAAAIRHPM
                     VAAVKLWALAHLFVRGDLASLILFGGLLVWAVVDRISLKKRERAGLVTIRAGGIANDL
                     IAIAIGLVLFALMLRWGHAALIGIALMSK"
     gene            1102..2064
                     /locus_tag="PK264_20435"
     CDS             1102..2064
                     /locus_tag="PK264_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78271.1"
                     /translation="MSFERDRFPHPASFAPPDHAPTTQPLLSRLKRVDVRQPNLGDAL
                     EAQRIDSVIRDLTQVLGPNPAAGAATWIGQPVRPVPVDPQALGAEIERMIPNDGPYPA
                     PGELPPLPVMPSPPDAFADYSFVEADEERMPIPSTWRETSETPAEPWLSRQLASGLVG
                     LGVGLAVILPLTLWVLGSIGGPGAGPTAATTSDPIVVAVRPQPVVAPARAVAASAADV
                     AIASEPIPVITTPAGDPTVLLASVELLVTGGEIGMARSLLLQSELRTHPAVIFALAET
                     FDPNMLAAWSVTGVNAEPAKARELYTKALDGGVDRARARIDALQ"
     gene            complement(2077..3168)
                     /locus_tag="PK264_20440"
     CDS             complement(2077..3168)
                     /locus_tag="PK264_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD78272.1"
                     /translation="MAVDVETIVVGAGVVGLAIGRALAARGQEVLVLERHALIGSETS
                     SRNSEVVHAGIYYPTRLCVAGKEMLYRFSAENGVSVARTGKLLVATSESQHVKLDALH
                     KTADANGVTDLVRLTGAEATAMEPELFCTAALLSPSTGVIDSHGLMVALEGHITTHGG
                     QVVLSTSVERATRGNDGTFRLHTGGAAPGTITCARLVLSPGLGASDLARTLALPSGYA
                     PPPTYYAKGQYYALSGKSPFSRHIYPMPDGAWLGLHVTVDIAGRCKFGPDIEWIPEID
                     YSFDVAKLDKFLGFIRSYYPGLDPSRLHADYTGIRPKLYREGEPVPDFRIDGPETHGA
                     SRLALLYGIESPGLTASLAIGELVADLVG"
     gene            3344..>3778
                     /locus_tag="PK264_20445"
     CDS             3344..>3778
                     /locus_tag="PK264_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78273.1"
                     /translation="MTGGRQIAWFSVGFLSALMLGLAAFSVGARAQTPPSTPSSTGDG
                     FITRVRPTLPTPVPRRPVPVPIPLIGDPSGDSMGRVPGTANRDGILADPDPSDDPDRP
                     ADANRPPVVDGDPNFPPIAEPQRDGVLVIPEAGANPDADDPIN"
     assembly_gap    3779..3829
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3830..5049
                     /locus_tag="PK264_20450"
     CDS             <3830..5049
                     /locus_tag="PK264_20450"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD78274.1"
                     /translation="ARAFDDGSAIEIEPILDRRPARLARFEPYDPIGLRWGQFIVFPE
                     IETAAVGRSNMFRSSASPAHDVGLEARPSVRVVSNWRVHALEFRANAVTSFWNRFPSE
                     DDRAYAFETRGRLDITRRTNIEALVGYQVEQEARGSVNAAAGADDRTDVVTSRAAVTL
                     NHRFNRLSIQLRGSVVDNDYQPVAAATGAIISNDARDLRTSEAAVRATWELKPALLVF
                     AESAFNWRTYDEAPASDGIRRDSSGDRIRLGVSFGNSGQIIRGEASIGYGTQRPDDHR
                     LPEIAGILIDANLAWRVSALTSILFTARTDVSESSVAGAGGALARSGGVEVRHAFLRN
                     LVTTGALRYTLTDYEGVSLAENELVAAMAAEYFLSRELTLFARYQHTEFDSTDAARNY
                     NLDEVRVGVRVRR"
     gene            complement(5068..>5542)
                     /locus_tag="PK264_20455"
     CDS             complement(5068..>5542)
                     /locus_tag="PK264_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78275.1"
                     /translation="ETRYGYAQEWIDLIRKICDALRLSPNDLLGSSGTAANDWIAGFS
                     ERQQAWHGPPPQPAGHLRATEQPELARGALCWLIAREFSALESEVPTSGGGLIASELQ
                     RSGALFKRLSERPFDAISAISQHPALSGQPSERTARIRQLIDQLAETLYQPGSTQ"
     assembly_gap    5543..5563
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5815..>6482)
                     /locus_tag="PK264_20460"
     CDS             complement(5815..>6482)
                     /locus_tag="PK264_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HRD78276.1"
                     /translation="QAEEIEFETRRFYERALERVSDASIRRLLGDLAAAEREHAGLAQ
                     RLGEEHLTADSKASEAEAQRRMFVLQVVQPGLAGLMDGSVSTLAPLFAAAFATHDTFQ
                     TFLVGMAASVGAGISMGFAEALSDDGALTGRGQPWVRGTITGLMTTVGGVGHTLPYLI
                     KDFWTATAFAVVVVLIELWAIAWIRARYMDTRFLRAAFEVVVGGLIVFGAGILIGSMG
                     GAH"
BASE COUNT         1102 a         2216 c         2081 g         1011 t
ORIGIN      
        1 gtccacggaa ggcgcgcggc acccgctact gcggcaagct cagcgagtgc cgcctcccag
       61 tcctcgcggg cgaggaccgt cacctgccgc cgcgggtact tggcgccacg gaaatcgccc
      121 tcgagcccgc gctcgcgcgt gacgaggcag cgcccggcct cgatcatcgg cgccttggtg
      181 cgagcccgcg tcgccagcga tagcagccga ccggccatgg cacctacttc gacatcaggg
      241 caataccgat gagcgcggcg tgcccccagc ggagcatcag tgcgaagagc acgagaccga
      301 tggcgatcgc gatcaggtcg ttggcgatgc caccggcacg gatggtgacg agcccggccc
      361 gctcgcgctt cttgagcgag atgcgatcga cgacggccca gacgagcaac ccgccgaaca
      421 ggatgagcga tgccaggtcg ccccgcacga agaggtgcgc cagcgcccag agcttcacag
      481 ccgcgaccat cggatggcgg attgctgcgg caatgggccc tttgaggtaa gccatgacga
      541 gcagcgggaa gaccgggagc atgagcgtgt aggcggcatg ccggccccag cccggcggcg
      601 tccagagcgg aacgtgcttg gccttcgccc atgccatcac cacgagcgcc aggcccgcaa
      661 gcgatatgag cgagaagacg cccttgtagc caccctcgcc gaacctgccg acgaggctcg
      721 cgcggagctg cggctgcgtc gggaccaggt gaatggcgat gaagagcgcg aggcccaacg
      781 tgagcagcag catctttcct cctcggtcga aatcgaagtc gggcgacgct gttcgcgttg
      841 ccctatcatc gactgtgccg tcaaccaatc ttcgctgcaa gttgccgacg cgcgatctga
      901 agcggtcagg ccctcacgaa gcgtgttgcc atggttcgaa cactattttc gaattcttgt
      961 gaggtcatca gggcttagga aagtgtgacg tcgcagagcc gaatggccgg tcgcttgtgg
     1021 ggggactttc cagctctggc acgaagtttg tactgcgtat cggggcgtcg tagcggtccg
     1081 atgagtagga ggggggcgtc catgagtttc gagcgcgatc gcttcccgca tccggccagc
     1141 ttcgctccgc ccgatcacgc tccgacgacc cagcctctcc tcagccgcct caaacgcgtc
     1201 gacgtccgtc agccgaacct cggcgatgct ctcgaggccc agcgcatcga ctccgtgatc
     1261 cgcgatctca ctcaagtcct cggaccgaac ccggcggccg gcgccgcgac gtggatcggc
     1321 cagcctgtgc gacctgttcc agtcgaccct caggcgctcg gcgccgagat cgagcggatg
     1381 atcccgaacg atggtcccta tccggccccg ggcgagttgc cgccgctccc cgtcatgccg
     1441 agccctccgg acgcattcgc cgactacagc ttcgtcgagg ccgatgagga gcgcatgccc
     1501 atcccgtcga cctggcggga aacgtcggag acgccggctg agccctggct ctctcggcag
     1561 ctcgcgagcg gcctcgtcgg cctcggtgtc ggcctcgcgg tcatcctgcc tctgacgctc
     1621 tgggtgctcg gcagcatcgg cggcccgggc gccggaccga ccgccgccac gacctccgat
     1681 cccatcgtgg tcgccgtgcg tccgcagccc gtggtcgcgc cggcgcgagc ggtcgccgca
     1741 agcgctgctg acgtggcgat agcctccgag ccgatcccgg tgatcacgac gccggcaggc
     1801 gatccgacgg tgctgctcgc aagcgtcgag ctccttgtga ccggcggcga gatcggcatg
     1861 gcgcgctcac tgctcctgca gagcgagctc aggacgcacc ccgccgtgat cttcgcgctc
     1921 gccgagacct tcgaccccaa catgctcgcc gcctggagcg tcaccggagt caacgcggag
     1981 cccgccaagg cgcgcgaact gtatacgaag gcgctcgacg gcggcgtcga ccgcgcgcgc
     2041 gctcgcatcg acgcgctgca gtaggcgcgg ccgacgtcag ccgaccaggt cggccaccaa
     2101 ctcgccaatc gcgagcgatg ccgtgaggcc tgggctctcg ataccataga ggagcgcgag
     2161 gcggctcgca ccgtgggttt ccgggccatc aatgcggaag tccggcaccg gctcgccttc
     2221 ccgatagagc ttcgggcgga ttcccgtgta gtcggcatgg agccggctcg gatcgagccc
     2281 ggggtagtaa ctgcgtatga agcccaggaa cttgtcgagc ttggcgacat cgaacgagta
     2341 gtcgatctcc ggaatccact cgatgtcagg gccgaacttg caccgcccgg cgatgtcgac
     2401 cgtcacatgg aggccgagcc aggcgccatc cggcatcggg tagatgtggc gggagaaggg
     2461 cgacttgccc gagagcgcgt aatactggcc cttggcgtag taggtgggcg gcggcgcgta
     2521 tcccgaaggc agggcgagcg tccgcgccag atcggaggcg ccaaggccgg gcgagaggac
     2581 gaggcgggcg caggtgatcg tgcccggcgc ggcgccgccc gtgtgcaggc ggaacgtccc
     2641 gtcgttgccg cgggtcgcgc gctcgaccga ggtcgaaagc accacctggc cgccgtgcgt
     2701 cgtgatgtgg ccttcgagcg cgaccatcag cccatggctg tcgatcacgc ccgtcgatgg
     2761 cgagaggagc gctgcggtac aaaagagctc gggctccatg gcagttgcct cggcgcctgt
     2821 gaggcgtacg agatcggtga cgccgttggc atccgcggtc ttgtggagcg catcgagctt
     2881 cacgtgctgg ctttccgacg tcgcgaccag cagcttcccc gtccttgcga ccgagacgcc
     2941 gttctcggcg ctgaagcgat agagcatctc cttgccagcg acgcagaggc gggtcggata
     3001 atagatgccg gcgtgcacga cctcgctgtt gcgcgaactc gtctccgagc cgatcagcgc
     3061 gtgccgctcg aggacgagaa cctcctgccc ccgcgccgcg agggctcgcc caatggccag
     3121 gccgaccaca cccgcgccga cgacaatcgt ttcaacatcg acggccattc cgtcctgctc
     3181 ctcggcgccc gaactccaga gcgctgcaat gacataattc gcgcccggac cacaaccctg
     3241 cgggcgagcg gagtggatcg tccggaaacg gctcattaac cagatcaccg ctatcgtccg
     3301 ctccgtgtgg cgtctctcgt gcagtgcggc aatggagcgc ccggtgaccg gtggtcgtca
     3361 aattgcgtgg ttcagcgtcg gcttcctgtc cgcgctcatg ctcgggctcg ccgcgttctc
     3421 ggtcggcgcg cgcgcacaga ccccgccatc cacgccctcc tccaccggcg atggcttcat
     3481 cacccgcgtc cgcccgaccc tgccgacgcc cgtaccgcga cgtccggtgc cagtccccat
     3541 tcccctgatc ggcgaccctt caggcgacag catgggccgc gtgccaggca cggccaaccg
     3601 cgatggcatt ctcgccgacc ccgacccgag cgacgatccc gatcgtccgg ccgacgccaa
     3661 ccgcccgccg gtcgtcgacg gcgacccgaa cttcccgccc atcgccgagc cgcagcgcga
     3721 cggtgtgctc gtcattccgg aggccggcgc caacccggac gccgacgacc cgatcaacnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgctcgtgcc
     3841 ttcgacgacg gcagcgccat cgagatcgag ccgatcctgg atcgccggcc ggcccgccta
     3901 gcccgcttcg agccctacga cccgatcggg ctccgctggg gccagttcat cgtcttcccg
     3961 gaaatcgaga cggcggcggt cggtcgctcg aacatgttcc gctcgagcgc aagcccggcc
     4021 cacgacgtcg ggctcgaggc gcgcccctcg gtacgcgttg tcagcaactg gcgcgtgcat
     4081 gcactcgagt ttcgcgcaaa cgcagtgacg agcttctgga accgcttccc gagcgaggat
     4141 gatcgcgcct atgctttcga gacgcgcggc cgcctcgaca tcacgcggcg gaccaacatc
     4201 gaggcgctcg tcggctatca ggtcgagcag gaggcacgcg gctcggtcaa cgcggccgcc
     4261 ggcgccgatg atcgcaccga cgtcgttacg tcacgcgcgg cagtaaccct caaccatcgg
     4321 ttcaatcggc tgtcgattca gctgcgcggc agtgtcgtcg ataacgacta tcagccagtc
     4381 gctgcggcca ctggcgcgat catctcgaac gatgctcgcg acctgcgcac gagcgaggcc
     4441 gcggttcgcg cgacatggga gctgaaaccg gcgctgctgg tctttgcgga aagtgccttc
     4501 aactggcgga cctacgacga ggcgccggcg agcgacggca tccgccgtga ttcgagcggc
     4561 gaccgcatcc gcctcggcgt ctcgttcggc aacagcggac agatcattcg cggcgaggcg
     4621 agcatcggct acggcaccca gcgacccgac gatcaccgcc tccccgagat cgccggcatt
     4681 ctcatcgacg ccaatctggc ctggcgcgtc tcggcgctga cctcgatcct gttcaccgcc
     4741 cgaaccgacg tgtcggagag cagcgtggct ggggcaggcg gggcgcttgc gcgcagcggc
     4801 ggtgtcgagg ttcggcacgc cttcttgcga aatctcgtta ccacgggcgc gctccgctac
     4861 acgctcaccg actacgaagg cgtgagcctc gccgagaacg agctcgtcgc ggcgatggcc
     4921 gccgaatact tcctcagccg cgagctgacg ctgtttgcgc gctaccagca caccgagttc
     4981 gattccactg atgcggcacg caactacaac ctggacgagg tccgcgtcgg tgtgcgcgtc
     5041 agacggtagg cccctgccgc agcgaggcta ctgagtggag cccggctgat acagcgtctc
     5101 ggcgagctgg tcgatcagct gacggatgcg agccgtccgc tcgctcggct gcccggataa
     5161 agccggatgc tgcgagatcg cgctgattgc atcgaaggga cgttccgaaa gacgcttgaa
     5221 gagcgcgccc gaacgctgca gctcgctggc gatcaaaccg ccgcccgacg tcggcacctc
     5281 ggattccagt gccgagaact cgcgggcgat gagccagcag agcgcacctc gcgcaagctc
     5341 gggctgctca gttgcgcgca ggtggccggc cggctgaggc ggggggccgt gccaggcttg
     5401 ctggcgctcg ctgaagcccg cgatccagtc attcgccgcg gtgccgctgc tcccgagcag
     5461 gtcgttcggc gacaggcgca gcgcatcgca gatcttgcgg atcaggtcga tccattcctg
     5521 cgcatagccg tagcgggttt cgnnnnnnnn nnnnnnnnnn nnnagggcct gggcaaggct
     5581 tcgtgccgtg cggaaaccgc gcggaaccct cagttctcgc aagcgcttgg caaactcgtg
     5641 ccgcgctgta tgtccggccg tgtctctcat ggtgagagaa tttgcagaaa aatcggccga
     5701 agtaatctgc aatattcgcc aatacggcag aatctggaat acttatctat actataagta
     5761 gaaataaaca acctacggtt gtacgcagat cagctttgcg aagattgtcg atcgtcagtg
     5821 ggcgccgccc atgctgccaa tgaggattcc tgcgccaaaa acgatgagac ccccgaccac
     5881 gacctcgaat gcggccctca gaaaacgcgt atccatgtaa cgagcccgta tccaggcaat
     5941 ggcccagagc tcgatcagta ccacgacgac ggcgaacgcc gtcgcggtcc agaaatcctt
     6001 gatcaggtac ggaagggtat gaccgacgcc gccgaccgtg gtcatgagcc ctgtgatcgt
     6061 gccgcgcacc cacggctggc cgcggccggt gagcgcgcca tcgtccgaaa gcgcctcggc
     6121 aaagcccatc gagatgccgg ccccaaccga ggccgccatg ccgaccagga acgtctgaaa
     6181 ggtatcatgc gtagcaaatg ccgcggcaaa gagcggggcg agcgtcgaca ccgagccgtc
     6241 catgagtccg gcgagccccg gctggaccac ctggagtacg aacatgcgac gctgcgcctc
     6301 tgcctcgctc gccttactgt ccgctgtgag atgctcctcg ccaagccgct gcgcgaggcc
     6361 cgcatgctcg cgctcggcgg cggcgagatc gccgagcagg cggcggatgc tcgcgtccga
     6421 cacccgctcc agcgcacgct catagaagcg gcgcgtctcg aactcgatct cctcggcctg
     6481 ct
//
LOCUS       DAORNH010000269         6474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8861_length_6474_cov_2.408319, whole genome shotgun sequence.
ACCESSION   DAORNH010000269 DAORNH010000000
VERSION     DAORNH010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8861_length_6474_cov_2.408319"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..730)
                     /locus_tag="PK264_20465"
     CDS             complement(131..730)
                     /locus_tag="PK264_20465"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRD78277.1"
                     /translation="MSFKLPDLPYAYDALAPFMSAETLQFHHDKHHLAYVDNGNKLKA
                     GTKFEAMSLEDICKAAYAEKNAGLVNNAGQHYNHNHFWKWMKKGGGGKSLPGKVDALV
                     TAYGGFDKVRTDFVQAGVTQFGSGWCWLAVKGGKLEVMKTPNGENPLMHGASPILGCD
                     VWEHSYYIDYRNARPKYLEAWFDNLVNWGYVEEMLGHAK"
     gene            982..2334
                     /locus_tag="PK264_20470"
     CDS             982..2334
                     /locus_tag="PK264_20470"
                     /inference="COORDINATES: protein motif:HMM:NF018938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HRD78278.1"
                     /translation="MREFRLVSTSGICGYGFPEESLKAAMKREPHMIGVDGGSTDPGP
                     HYLGSGKCLNSRMAVKRDIALMMDAALSARVPMVISTCGGAGGEPHLQATAEIVHEIA
                     REKGHKFKLALIHAEQPKSFVKEKLAKGKVKPLKAVPELTAETVDKASRIVGMMGPEP
                     FIQALDNGAEIVLAGRDPAPWAACATRAQLPPAPAWFAGKLLECGATPSIPKGHDCLL
                     ATVRDDHVIVEALNPIRKCTPMSVANHSLHENPSPCIHVEPGGVLDTTNCRFEAESDR
                     AVRISGMRWTPTPYTVKLEGAELAGYRTITMCGTRDPGLLGQWESFLKTVRQNVTDKA
                     RAFGVEPDQYQLIFRVYGRDGVMGEWEPVSRSQSHELGVLIEVVGKTQEIASAVLAIA
                     RVNALHTDFPGRLCKEGNMAFPFSPSDIDAGATYRFSVFHIVEDVDPLSMFPIEYQMV
                     "
     gene            2340..2651
                     /locus_tag="PK264_20475"
     CDS             2340..2651
                     /locus_tag="PK264_20475"
                     /inference="COORDINATES: protein motif:HMM:NF025688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HRD78279.1"
                     /translation="MPKVREIAQVCKSKNAGPFEVTIDVVFADKAMFERVKKTGVLSA
                     ELMARLYHVPADDILFTVYDAGLAFKATLPRRIPAGDVGDTDVYGAQQHAPLLEVDIP
                     V"
     gene            complement(2738..3175)
                     /locus_tag="PK264_20480"
     CDS             complement(2738..3175)
                     /locus_tag="PK264_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD78280.1"
                     /translation="MYKHILIATDGSDLAQKAVGQGLSLARALDAKAIVVMVTEPLRT
                     VASGELMVAFPADEYEKTAAASAAKVLASAVDVARKVGIQTETLHVKEQLPAEGIVET
                     AKRVGADLIVMASHGRRGLSRMLLGSQAAKVVQLSEVPVLICR"
     gene            complement(3267..3758)
                     /locus_tag="PK264_20485"
     CDS             complement(3267..3758)
                     /locus_tag="PK264_20485"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRD78281.1"
                     /translation="MPNIDIKKNQSPAPVYSGDVFQAMRGEIDRLFDSFGRGFPAMPS
                     LFARGGGNLLPCHIDVRDEGAALVVEAEIPGVDEKDVTVTVANGVLTIAGEKKSEREE
                     KDADYYVSERSFGSFQRSLRLPPEIDEEKIEARFEKGVLRVRAAKRAEAVKAAKKIEI
                     TKS"
     gene            3964..5550
                     /locus_tag="PK264_20490"
     CDS             3964..5550
                     /locus_tag="PK264_20490"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD78282.1"
                     /translation="MAKADPAHTGAADDQTETVRALSDPALYPGRPGRIDRHETHGAL
                     VFLASDRAYKLKRSVKFAYMDLSTRDKRRRMLEREYEINRRFAPDLYLGVMAITRGRD
                     GALRLGGDGPPVDHVLEMRRFPEDQLLSAITRRRALTDAIASDLAAAVLESHRTAPIA
                     SVADADRRIARTIESISAELETLAAILQPLDPREFAARANMQLDRARSVLCARGEAGM
                     VRRCHGDLHLNNIVLIDGRPTLFDALEFDEALATIDTLYDLAFLLMDLDHAGARATAS
                     HILSRYLAHAPSPLDIDGLVALPLFLGLRSAIRAMTSAARARLDGSEAARPYSEAACS
                     YFADALRYLAPSEPRLIAIGGRSGTGKTTLARALAATIDPPPGALHLRTDVERKRLSG
                     VAETERLSPESYTPAASRRVYDAVLTKARAALAAGHSVVIDAVFLRASERHEIEAIAQ
                     SLGRPFQGLWLTARRDVLIDRVSGREGDASDATAAVVELQLASDAGPITWSVIEAGSG
                     PEVTLSRGREALRPLRARTD"
     gene            5605..>6474
                     /locus_tag="PK264_20495"
     CDS             5605..>6474
                     /locus_tag="PK264_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRD78283.1"
                     /translation="MSVRNLRYLLEPRSVAFLGASAREGSLGRIISANLTKGGFKGPV
                     WLVNPKRGEIAGQRVFGSVTELPAAPDLAVIATQPATIPTLVGELGAKGTRAAVVISA
                     GMTGELKQAMLDAARPHLLRIQGPNCLGLILPPLGLDASFSHLAPPAGDLALVSQSGA
                     LITSIVDWAAARRIGFSHVISLGDMADVDFGDLLDYLAAAPRCRAILLYIEQLTHAAK
                     FMSAARRAARAKPVIAIKAGRHAEGARAAMSHTGALAGADAAYDAAFRRAGVLRVNEL
                     EDLFNAAEMIARVP"
BASE COUNT         1072 a         2298 c         1995 g         1109 t
ORIGIN      
        1 gcagaagtgg aaggccatcc gcctcgtcaa acagtcatga attgaattgc cctggaacgg
       61 cgccgggtgc aaggctgtcc gccctgcacc cggagcccaa ttcaccatcg cctgaatacc
      121 ggcgctcaga tcacttcgcg tggccgagca tctcctcgac gtaaccccag ttgacgaggt
      181 tgtcgaacca cgcctcgagg tacttcggcc gggcattgcg atagtcgatg tagtagctgt
      241 gctcccacac gtcgcagccg agaatcgggc tcgcgccgtg catcagcggg ttctcgccgt
      301 tcggcgtctt catcacctcg agcttgccgc ccttgaccgc gagccagcac cagcccgagc
      361 cgaactgggt gacgcccgcc tgcacgaaat cggtgcgcac cttgtcgaag ccgccatagg
      421 ccgtcaccag cgcatcgacc ttgcccggga ggctcttgcc gcccccaccc ttcttcatcc
      481 acttccagaa gtggttgtga ttgtagtgct gaccggcgtt gttgacgagg ccggcgttct
      541 tctcggcgta agccgccttg cagatgtcct cgaggctcat cgcctcgaac ttcgtgccgg
      601 ccttcagctt gttgccgttg tcgacatagg cgaggtggtg cttgtcgtga tgaaactgca
      661 gcgtctcggc cgacatgaag ggcgcgagcg cgtcataggc atatggcaga tcgggaagct
      721 tgaacgacat ggcgagcctc aactttaatg gccgggggag ctattcgagt ggtccggacc
      781 ctaatggggt gcgctgccga tgacaagcgc gggtcgactg cacgggctgc cggcccggaa
      841 cggcatctgc cttggccgcc gagggtccat gccggcgtgt catagctcaa ggcctgactt
      901 tgccttgacc tccctttgca gcgaccgcta accgttgtcg ggcacgccca catgggcctc
      961 agccgcactc tggaggacgt catgcgcgaa ttccggctgg tctcgacgag cggcatttgc
     1021 ggctatggat ttcccgagga atcactgaag gccgccatga agcgcgagcc gcatatgatc
     1081 ggcgtcgacg gcggctcgac cgatcccggc ccccactatc ttggctccgg gaaatgcttg
     1141 aactcgcgca tggcggtgaa gcgcgacatc gcgctgatga tggacgccgc cctctccgcg
     1201 cgcgttccca tggtgatctc cacctgcggc ggcgccggcg gcgagccgca tctccaagcc
     1261 acggccgaga tcgtgcacga gatcgcccgc gagaaaggcc acaagttcaa gctcgcgctc
     1321 attcatgccg agcagccgaa gagcttcgtc aaggagaagc tcgccaaggg caaagtgaag
     1381 ccgctgaagg ccgtgcctga actcacggcg gagaccgtcg acaaggcctc ccgcattgtc
     1441 ggcatgatgg ggccggagcc gttcatccag gccctcgaca acggcgccga gatcgtgctc
     1501 gccggccgcg atcccgcccc ctgggccgcg tgcgcaacac gcgcacagtt gccgcccgca
     1561 cccgcctggt tcgccggcaa gctcctcgaa tgcggggcga cgccctccat cccgaagggc
     1621 cacgactgcc tgctcgccac cgtccgcgac gaccacgtca tcgtcgaagc cctgaacccg
     1681 atccgcaagt gcacgccgat gtcggtcgcc aaccactcgc tgcacgaaaa cccgagcccc
     1741 tgcatccacg tcgagccggg cggcgtgctc gatacgacca actgccgctt cgaggcggaa
     1801 tcggatcgcg ccgtgcgcat ctccgggatg cgctggaccc cgacgcccta caccgtgaag
     1861 ctcgagggcg ccgagctcgc cggctaccgc accatcacga tgtgcgggac gcgcgatccg
     1921 ggcctccttg gccaatggga gagcttcctc aagaccgtac gccagaacgt cacagacaag
     1981 gcgcgggcct tcggcgtcga gcccgatcag taccagctga tcttccgcgt ctatggccgc
     2041 gatggcgtga tgggcgaatg ggagcctgta agccgatccc agtcgcacga gctcggcgtg
     2101 ctcatcgagg tggtcggcaa gacgcaggag atcgccagcg ccgtgctcgc catcgcccgt
     2161 gtgaatgcat tgcacaccga cttcccgggc cgcctctgca aggagggcaa catggcgttc
     2221 cccttctcgc cctccgacat cgatgcgggc gccacctacc gcttctccgt cttccacatc
     2281 gtcgaggacg tcgacccgct gtcgatgttc ccgatcgaat accagatggt gtgacaccga
     2341 tgcccaaggt ccgcgagatc gctcaggtct gcaagagcaa gaatgccggc cccttcgagg
     2401 tgacgatcga cgtcgtcttc gccgacaaag ccatgttcga gcgtgtgaag aagaccggcg
     2461 tgctcagcgc cgagctgatg gcgcggctct accatgtgcc ggccgacgac atcctgttca
     2521 ccgtctatga cgccggcctc gccttcaagg cgacgctccc gcgccgcatt cccgcgggcg
     2581 acgtcggcga taccgacgtc tacggcgccc agcagcacgc ccctctcctc gaggtcgata
     2641 tcccggtctg agcggagcct tgcggctctc ttcaatttcg ggtccaggga ccagtccctg
     2701 gcggggtcga ggggcagcgc acctcgttct tcttcgttca gcggcagatg agaacaggca
     2761 cctcgctcag ctgcacgacc tttgccgcct ggctgcccag cagcatgcgc gagaggcccc
     2821 gccggccatg cgatgccatc acgatgagat cggcgccgac gcgctttgcc gtctcgacga
     2881 tgccctcggc cggcagctgc tccttgacgt gcagcgtctc ggtctggatg ccgaccttgc
     2941 gagcaacatc gacggcgctc gccaagacct ttgcagcact cgccgcggca gtcttctcgt
     3001 actcgtctgc cgggaaggcg accatcagct cgcccgaggc gaccgtgcgc agcggttccg
     3061 tcaccatcac gacgatcgct ttcgcgtcga gcgcgcgggc gagcgatagg ccttgaccaa
     3121 cggctttctg cgcaagatcg gagccgtctg tcgcgatcag gatgtgcttg tacatgggtt
     3181 ctctccaagg cccctgtgct cgaaagcgtg ttgtggagtt cgcccgccaa cggcagccag
     3241 ccgattgccg ggcgagtgct ccggtgtcac gatttggtga tctcgatctt ctttgccgcc
     3301 ttcacggcct cggcccgctt tgccgcgcgc accctgagca cgcccttttc gaagcgggcc
     3361 tcgatcttct cctcgtcgat ctcgggcggg aggcggagcg agcgctggaa cgagccgaag
     3421 ctgcgctcgc tcacgtagta gtcggcgtcc ttctcctcgc gctccgactt cttttcacct
     3481 gcaatggtga gaacgccgtt ggcaactgtc accgtgacgt ccttctcgtc gacgcccggg
     3541 atttcggcct cgacgacgag tgccgcgcct tcgtcgcgca catcgatgtg gcacggcagg
     3601 agatttccgc ccccgcgcgc gaacaacgac ggcatggccg gaaaccctcg accgaagctg
     3661 tcgaacagtc gatcgatctc gccgcgcata gcctggaaga cgtcaccgga gtagacagga
     3721 gccggtgact ggttcttctt gatgtcgatg ttaggcatcg gtacctctcg ttcgcttgat
     3781 gaccacaaga cttgatgacc aagcggcctc gtcatgagag ccgttgctga ttgagataag
     3841 tacagctggc cgaatttcca ttgatgtggc tcaaaccgga cccgatcgtc ccgaggcaag
     3901 gctcgtaccg cgcgccatcc ggaggacgcg ttggagcgtc acgcagcaac gggagcacgt
     3961 cccatggcga aggccgatcc cgcgcatact ggtgcggcgg acgaccagac cgagacggtt
     4021 cgcgccctct ccgatcccgc cctctatcct ggcaggccgg gccgcatcga ccgccacgag
     4081 acgcacggcg ccctcgtctt cctcgcgagc gatcgggcct acaagctgaa gcgctcggtc
     4141 aaattcgcct acatggacct ttctacgcgg gacaagcgcc gccgtatgct cgagcgcgaa
     4201 tatgagatca accgccgctt cgcgcccgac ctgtacctgg gcgtcatggc gatcacgcgc
     4261 ggccgcgacg gtgccctgcg gctcggcggc gacggcccgc cggtcgacca cgtcctcgag
     4321 atgcgacgct tccctgagga ccagttgctg agcgctatca cccgcagacg cgccctcacc
     4381 gacgccatcg ccagcgacct cgccgccgcc gtcctcgaga gccaccgcac ggccccgatc
     4441 gcctcggtag cggacgcgga tcgtcggatc gcgcgaacga tcgagagcat ctcagccgag
     4501 ctcgagacac tcgcagccat tctccagccg ctcgaccctc gcgagttcgc cgcgcgcgcc
     4561 aacatgcagc tcgaccgcgc gcgcagtgtg ctctgcgctc gtggcgaggc cggcatggtg
     4621 cggcgctgtc atggcgatct ccacctcaac aacatcgtcc tcatcgacgg ccggccgacg
     4681 ctcttcgacg cactcgagtt cgacgaggcg ctggcgacga tcgacacgct ctacgacctc
     4741 gcctttctgc tgatggacct cgaccacgcc ggtgcccgtg ccaccgcgag ccacatcctg
     4801 agccgctatc tcgcgcacgc gccgagccca ctcgatatcg acggtctggt cgcactccca
     4861 ctgttcctcg gcctcagaag cgccatccgc gccatgacga gcgccgcgcg cgcgaggctc
     4921 gacggaagcg aagcggcaag gccatactcg gaagccgcct gctcctattt tgccgacgcg
     4981 ctccgctacc tcgccccatc cgagccgcgg ctgatcgcca tcggcggtcg ctcgggcacc
     5041 ggaaagacca cgctcgcgcg tgcgctagcg gcaacgatcg acccgccgcc gggtgcgctg
     5101 catctccgca ccgacgtgga gcgcaagcgc ctctccggcg tggccgagac cgagcggctg
     5161 tcgcctgaga gctatacgcc ggccgcctcc cgcagggtct acgatgccgt actcaccaag
     5221 gcgcgagccg cacttgccgc cggtcattcg gttgtcatcg acgccgtatt ccttcgggcg
     5281 agcgagcgcc acgagatcga ggcaatcgcg caaagcctcg ggcggccttt ccaagggctt
     5341 tggctcacgg cgaggcgcga cgtactcatc gaccgcgtca gcgggcggga gggcgatgcc
     5401 tccgatgcca cggccgccgt cgtcgagctc cagctggcga gtgacgccgg cccgatcacg
     5461 tggtcggtga tcgaggccgg cagcggcccc gaggtcactc tcagccgcgg ccgggaggca
     5521 ctgcgtcctc tcagggctcg caccgactga tcgaacgata cgcgcggcca cggctgattg
     5581 ctggacgcga cgacgggagg ccacatgtct gtccgcaatc tgcgttacct gctcgagcct
     5641 cggtccgtcg cctttctcgg cgcgagcgcg cgcgaaggaa gcctcggtcg gatcatctcg
     5701 gcgaacctca cgaaaggcgg cttcaaggga ccagtttggc tcgtcaatcc gaagcgcggc
     5761 gagatcgccg gacagcgagt cttcggcagc gtcacggagc tgccggccgc gcccgatctc
     5821 gccgtcatcg ccacgcaacc ggcaaccatc cccacgctcg tcggcgagct cggcgccaaa
     5881 ggcacgcgcg ccgcagtcgt catctcggcg ggaatgacgg gcgagctcaa gcaagcaatg
     5941 ctcgatgccg cgcgacccca cctcctgcgc atccagggcc ccaactgcct cggcctcatt
     6001 ctgccgcctc tcggtctcga cgcgagcttt tcgcatctgg cgccgccggc aggcgacctc
     6061 gccctcgttt cgcagtcagg cgcgctcatc acctcgattg tcgactgggc ggcggcgcgg
     6121 cgcatcggct tctcgcacgt catctcgctc ggcgacatgg cggacgtcga tttcggcgac
     6181 ctcctcgatt atctcgccgc cgccccccgc tgccgggcaa tcctcctcta catcgagcag
     6241 ctgacgcatg ccgcgaagtt catgtcggcc gcccgccgcg ccgcgcgcgc caagcccgtg
     6301 atcgccatca aggccggccg gcatgccgaa ggggcgcggg ccgcaatgtc gcacaccggt
     6361 gcgctcgccg gcgcggatgc tgcctacgat gccgccttcc ggcgcgcggg cgtgctgcgc
     6421 gtcaatgagc tcgaggatct cttcaatgcc gccgagatga tcgcgcgcgt gccg
//
LOCUS       DAORNH010000270         6425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8947_length_6425_cov_2.448352, whole genome shotgun sequence.
ACCESSION   DAORNH010000270 DAORNH010000000
VERSION     DAORNH010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8947_length_6425_cov_2.448352"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PK264_20500"
     CDS             <1..376
                     /locus_tag="PK264_20500"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HRD78284.1"
                     /translation="SWDRDGQLASAKGNAMIKNFEDVQKFGQVNVDTALKFFGDWNKG
                     WQAIAAEITDYSKRAFEDGTATFEKLAAAKSVEQAFEIQTSYVKRSYDEYMHQLSKIG
                     NMYAEITKEAYRPLERVMPTRR"
     gene            496..>789
                     /locus_tag="PK264_20505"
     CDS             496..>789
                     /locus_tag="PK264_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRD78285.1"
                     /translation="MGRRRKKGFPIDGWVILDKPVGMTSTQAVGKVRWLYDAQKAGHA
                     GTLDPLATGILPIALGQATKTVPFAVDGVKAYRFAVRWGAETDSDDSDGTVTAT"
     assembly_gap    790..888
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <889..1413
                     /locus_tag="PK264_20510"
     CDS             <889..1413
                     /locus_tag="PK264_20510"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRD78286.1"
                     /translation="GQRAYDLARSGEEVEIEARPVSIDRLDLIDAQNADEAILEAVCG
                     RGTYVRAIARDLGRLLGCRGHVTALRRTRVGDFNESRSVSLDALIAACEADDGGAALA
                     SYLLPVEAALSDIPHLAVQQSDAARIARGQPIIIRGRDAPTDSPDAYVTTKGTLLALG
                     YIERGEFHPTRVFN"
     gene            1490..2494
                     /gene="paaA"
                     /locus_tag="PK264_20515"
     CDS             1490..2494
                     /gene="paaA"
                     /locus_tag="PK264_20515"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HRD78287.1"
                     /translation="MYSQALNEKDKGSEHGGPEDAVRLARFEARCAADDKIEPNDWMP
                     AAYRRTLVRQISQHAHSEIIGMQPEGNWVTRAPSLRRKAALLAKIQDECGHGLYLYAA
                     AETLGVGREELLDQLHTGKAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQIPLCRCS
                     FGPYARAMIRICKEESFHQRQGYEIMLTLCRGTAKQRAMAQDALDRWWWPCLMMFGPS
                     DTESIHSADSSRWRIKRFSNDELRQKFVDATVPQGHYLGLDFPDPELKRDPATGHWRF
                     GEIDWSEFKRVLAGDGPCNRERLANRNAANEDGAWVRAAALAYAAKREARAAARVAAE
                     "
     gene            2504..3448
                     /locus_tag="PK264_20520"
     CDS             2504..3448
                     /locus_tag="PK264_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRD78288.1"
                     /translation="MPNEPAVTPRDLAELLIWYVELGVDCALGEAPVDWLVRGDIAPG
                     HGFRWPPADAAPDAVAPSSDSPQAERHVSAPPAQRHAPPLAGRPPSTFPAPSPPPRTV
                     TPQPAAPVSADAGVMAARIAAASAASLEDLGRLLSGFEGCGLKATAKSLCLYRGAARA
                     RTMIIGEAPGREEDISGRPFVGPAGQLLDKMLAAIGLAEGDVHITNTVYWRPPGNRTP
                     TPQEVLVCRPFLDRQIELVAPDVIVTLGGPATKSILDVADGIMRVRGKWRTLTHAGRS
                     IKVMATLHPAYLLRTPAAKRMAWRDLLAIEAELGEPSK"
     gene            complement(3528..4424)
                     /locus_tag="PK264_20525"
     CDS             complement(3528..4424)
                     /locus_tag="PK264_20525"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRD78289.1"
                     /translation="MRPKARTLSLLAASAVGGITSLAISTAPAAAQEMLTLHNTRRAQ
                     HCAPAMRWSAALAASAQTWANRCQFVHSSSAERPGQGENLAWGTGSFATAVSSFGRWY
                     DEVSRYNFSAPGFSSATGHFTQIVWRGSTELGCAMAMCSGQAFWVCRYGPAGNITNPG
                     MFASNVLPTSCRTATPPPPPGNPTPGPGPTPTPGGSGEWSAFATDGRGRWGYATHRAN
                     AALASSGAISGCGGAGAGCRVFWTTRDRCVSYAESRSGGFWYAAGGGPTDAVARQNAI
                     RFCQSGTAPRGSCRTTGAWCRR"
     gene            complement(4664..6085)
                     /locus_tag="PK264_20530"
     CDS             complement(4664..6085)
                     /locus_tag="PK264_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRD78290.1"
                     /translation="MASGTRSGAIGRSREYVDGGRFEAELGRRVAIRTESQKFPDPRA
                     IAECRRYLDAEMIPAFDAMGFTCKIYDNPIPGQGPILLATRIEEASLPTVLGYGHGDV
                     VVGMDERWTKGKGPWVTARDGDRLYGRGTADNKGQHTLNMAALAAVMAERGGKLGFNA
                     KFMIEMGEEAGSKGLDALAAAEKGAFSADVLVASDGPRVKADRPTITLGCRGAMNFDL
                     IVDLRQGAHHSGNWGGALANPGFILAHALASIVGPKGELLVEELKAPPMSAATKASLV
                     DVELDGGADGPPVDDWYGEPGLKPVERVYAANTFNILAFTCGTPERPVNAIPPKAVAN
                     CQLRFIAGTRVADIIPALERHLARGGHTLVKVSPPPATNDGTFGASRIEPDHPWAEFV
                     RGSVQRSSNGKPAVIPSSGGSICNEVFTETLGMPTLWLPHSYASCSQHAPDEHILMGL
                     SRSAVELMAGLYWDIGDGKAPRR"
     gene            complement(6109..>6425)
                     /gene="rnpB"
                     /locus_tag="PK264_20535"
     ncRNA           complement(6109..>6425)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK264_20535"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1034 a         2208 c         2075 g         1009 t
ORIGIN      
        1 ttcgtgggat cgtgacggtc aactcgcatc agcgaagggg aatgccatga tcaagaattt
       61 cgaggacgtg cagaagttcg gccaggtcaa cgtcgacact gccctcaagt ttttcggcga
      121 ttggaacaag ggctggcagg caattgccgc cgagatcacc gactactcca agcgcgcgtt
      181 cgaggatggc acggccacct tcgagaagct cgccgcggcc aagtccgtcg agcaggcctt
      241 cgagatccag acgagctacg tgaagcgctc ctacgacgaa tacatgcacc agctctccaa
      301 gatcggcaac atgtacgccg agatcaccaa ggaggcttat cgccccctgg agcgcgtgat
      361 gccgacgcgt cgctgatcca ggctcagagc cgaagagacg gggcccggat cgatccgggc
      421 ccttttttag ttgcgccccc gccccaagtg cgggattgcc tcgcggcgag agcgagggag
      481 gctgacggga aacgcatggg gcggcgcagg aagaagggtt ttccgatcga cggctgggta
      541 atcctcgaca agccggtcgg catgacgtcg acccaggcgg tcggcaaggt tcgctggctc
      601 tacgatgcgc agaaggcggg gcacgccggc actctcgatc ccctcgcgac cggcatcctg
      661 ccgatcgccc tcgggcaggc gacaaagacc gtgccattcg ccgtcgacgg cgtgaaagcc
      721 taccgcttcg ccgtgcgctg gggcgccgag accgacagcg acgattccga cgggaccgtc
      781 acggcgaccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ccagcgcgcc
      901 tacgatcttg cccgtagcgg cgaggaggtc gagatcgagg cgcggccggt ctcgatcgac
      961 cgactggatc tgatcgacgc gcagaacgcc gatgaggcaa tcctcgaggc cgtctgcggt
     1021 cgcggcacct acgtgcgcgc catcgcccgc gacctcggcc gcctgctcgg ctgccgcggc
     1081 cacgtcacgg cgcttcgacg cacgcgcgtc ggcgacttca acgagagccg ctccgtctcg
     1141 ctcgacgccc tgatcgcggc ctgtgaggcc gacgacggcg gggccgcgct cgcgagctac
     1201 ctcctacccg tcgaggctgc tctctccgac attccccatc tcgcggtgca gcagagcgac
     1261 gccgcccgca tcgcgcgcgg ccagccgatc attatccgcg gtcgcgacgc cccgaccgat
     1321 tcgcccgacg catatgtcac gaccaagggc acgctcctcg cacttggcta catcgagcgc
     1381 ggcgagttcc acccgacgcg cgttttcaac tgaagctcgg cagggtcggc ctggccgtgg
     1441 tatgctcatc ggatggccga tgcggcgtcc caagagcgtg gagagatgca tgtactccca
     1501 ggccctcaat gagaaggaca agggcagcga gcacggcggc ccggaggatg ccgtccgcct
     1561 ggcccgcttc gaagcgcgct gcgccgccga cgacaagatc gagcccaacg actggatgcc
     1621 ggccgcctac cggcgcacgc tcgtccgcca gatctcacag cacgcccact ccgagatcat
     1681 cggcatgcag cccgagggaa actgggtgac gcgagcgccg tcgctccgtc gcaaggccgc
     1741 cctcctcgcc aagattcagg atgagtgcgg gcacggcctc tacctctacg ccgccgccga
     1801 gacgctgggc gtcggccgcg aggagctgct cgaccagctg cacaccggta aggccaagta
     1861 ctcctcgatc ttcaattatc cgacgctcac ctgggccgac atcggagcca tcggctggct
     1921 cgtcgatggc gcggctatca tgaaccagat cccgctctgc cgctgcagct tcggccccta
     1981 tgcccgcgcc atgatccgca tctgcaagga ggagagcttc caccagcgcc agggctacga
     2041 gatcatgctt acgctctgcc gcggcaccgc gaagcagagg gccatggcac aggacgcgct
     2101 cgatcgctgg tggtggccgt gcctcatgat gttcggccca tccgatactg agagcatcca
     2161 ctcggcagac tcctcccgct ggcgcatcaa gcgcttctcg aacgatgaac tccgccagaa
     2221 gttcgtcgac gcgaccgtgc ctcagggcca ctacttgggc ctcgactttc ccgatcccga
     2281 actgaagcgc gatcccgcga ccggccactg gcgcttcggc gaaatcgact ggtcggaatt
     2341 caagcgcgtg ctcgcgggcg acggcccatg caatcgcgag cggctcgcca atcgtaacgc
     2401 ggccaacgag gatggcgcct gggtgcgggc ggcggcgctc gcctatgccg caaagcgcga
     2461 ggcgcgcgct gcggctcggg tcgccgcgga atagccggcg atcatgccga acgagcctgc
     2521 cgtcacaccc cgcgacctcg ccgagctgct catctggtat gttgagctcg gggttgactg
     2581 cgcgctcggc gaggcgccgg tcgactggct cgtgcgaggc gacattgcgc cgggccacgg
     2641 cttccgttgg ccgccagcgg acgctgctcc ggacgcagtt gcgccttcaa gcgatagccc
     2701 tcaagccgag cgtcatgtat cggccccacc agcgcaacgg cacgctccgc cgctcgcggg
     2761 gcggccaccc tcgactttcc ccgcgccctc ccctccccca cgcaccgtga cgccacaacc
     2821 cgcggctccc gtttcggcag acgcaggcgt catggccgca cgcatcgctg ccgcgtcggc
     2881 cgcgtcgctc gaagatctcg ggcggctgct ctcgggcttc gagggatgcg gcctgaaggc
     2941 cactgccaag tcgctctgcc tctaccgcgg cgccgccagg gcgcgcacca tgatcatagg
     3001 cgaggcgcct ggacgcgagg aggatatctc cggcaggccc ttcgtcggcc cggccggcca
     3061 gctcctcgac aagatgctcg cagcgatcgg tctcgccgag ggcgatgtgc acatcaccaa
     3121 cacggtctac tggcgcccac ccggcaaccg cacgccgacc ccgcaggagg tgctcgtctg
     3181 ccgtccgttc ctcgatcgcc agatcgagct tgtagcacct gacgtcatcg tgacgctcgg
     3241 cggtccggcg acgaagtcga tcctcgacgt cgccgacggc atcatgcgcg tgcgcggcaa
     3301 gtggcgcacc ctcacgcacg ccggccgctc aataaaagtg atggcgaccc ttcatcccgc
     3361 atatctgctg cgcacgcctg ccgccaagcg catggcctgg cgggatctcc tcgccatcga
     3421 ggcggaactc ggcgaaccat cgaaatagcg ggtcaggcga caagcccctg gcagggcgct
     3481 cgaggtgttc gaggggcggc gcccctcgaa ctgcctttgc tcgggcccta tcggcggcac
     3541 caggccccgg tcgtccggca gctgcctcgc ggcgccgtgc cactctggca gaagcgaatg
     3601 gcgttctgcc gcgcgaccgc gtccgtcggt ccacccccgg ccgcatacca gaacccgccc
     3661 gagcggcttt ccgcatacga aacgcagcga tcgcgcgtcg tccagaagac ccggcagccg
     3721 gctcccgcac cgccgcagcc cgagatggcg cccgagctcg ccaaggcggc attggcgcga
     3781 tgcgtggcat aaccccagcg tccgcggccg tcagtcgcga aggccgacca ctcgccggaa
     3841 ccgcccggtg tcggcgtcgg cccgggacct ggagttggat tgccgggtgg cggcggcggt
     3901 gtggcggtgc ggcagctcgt tggcagcaca ttcgacgcga acattcctgg attggtgatg
     3961 ttgcccgccg gtccgtaacg gcagacccag aaggcctggc cgctgcacat cgccatcgcg
     4021 cagccgagct cggtcgagcc gcgccagacg atctgcgtga agtgaccggt cgccgagctg
     4081 aagcccggcg ccgagaagtt gtagcggctt acctcgtcgt accagcggcc gaacgacgat
     4141 acggccgtcg caaagctccc tgtaccccat gcgaggttct cgccctggcc gggacgctct
     4201 gcactggacg agtggacgaa ctggcagcgg tttgcccacg tctgcgctga agccgccagc
     4261 gccgccgacc agcgcatggc gggcgcgcag tgctgggcgc ggcgggtatt gtgcaacgtc
     4321 agcatctcct gtgcggcggc aggtgccgtg gagatcgcaa gagatgtgat gcccccgacc
     4381 gccgaggcgg caaggagtga aagtgtgcgg gcttttgggc gcatggcagc aatccctggc
     4441 gagccggggc tcgattgatc ggatggcgca aagtcttgtt cgagtcttgg ggacctcgtc
     4501 gctatccgaa cacggacgag gacgtcgcga agtaatatgg aaatgatact tctgtcagat
     4561 tggctctgat ctcgctcgaa gctagcgccg atcgcgcgcg tggacaaggg cgagccgggt
     4621 aacaccagga tcacccggtt caggtaccgt tcaggcgaca gcctcagcgc cgcggcgcct
     4681 tgccatcgcc gatgtcccag tagagtcccg ccatcagctc gaccgcgctg cggctgagac
     4741 ccatgaggat gtgctcgtcg ggtgcgtgct gcgagcacga ggcgtatgag tgcggcagcc
     4801 agagcgtcgg catgccgagc gtctcggtga acacctcgtt gcagatcgat ccgccagagg
     4861 aaggaatgac cgcaggcttg ccgttcgagg agcgctgcac tgagccgcgc acgaactcgg
     4921 cccagggatg atcgggctcg atgcgcgagg caccgaaggt accgtcgttg gtcgcgggcg
     4981 gtggcgagac cttgacgagg gtatgcccgc cacgcgcgag atgccgctcg agcgccggga
     5041 tgatatcggc gaccctggtg ccggcaatga agcgcagctg gcagttggcg acggctttcg
     5101 gcgggatcgc gttcacaggg cgctcgggcg tgccgcaggt gaaggcgagg atgttgaatg
     5161 tgttcgccgc atagacacgc tcgaccggct tgagccccgg ctcgccatac cagtcgtcga
     5221 ccggcggacc gtccgcgccg ccgtcgagtt cgacatcgac gagcgaggcc ttggtcgccg
     5281 ccgacatcgg aggagccttc aattcctcaa ccaggagctc gcccttcggg ccgacgatcg
     5341 aggcaagcgc gtgggccagg atgaagcccg gattggcgag cgcgccgccc cagttccccg
     5401 agtggtgcgc gccctggcgc agatcgacga tgagatcgaa attcatcgcg ccgcgacagc
     5461 cgagcgtgat cgtcggtcgg tccgccttga cgcgcggccc atcggaggcg acgagcacat
     5521 cggccgagaa cgcgcctttc tcggcggccg cgagcgcatc gagccccttt gaacccgcct
     5581 cctcgcccat ctcgatcatg aacttcgcgt tgaagccgag cttgcccccg cgctcggcca
     5641 tgacggcggc gagcgctgcc atgttgagcg tgtgctgccc cttgttgtcg gccgtgccgc
     5701 ggccatagag gcgatcgccg tcgcgtgccg tcacccaggg tcccttgccc ttggtccagc
     5761 gctcgtccat gccgacgacg acgtccccgt ggccgtagcc gagcaccgtc ggcaggctcg
     5821 cctcctcgat gcgcgtcgcg agcaggatcg gcccctgtcc cgggatcgga ttgtcgtaga
     5881 tcttgcacgt gaagcccatc gcgtcgaacg ccgggatcat ctcagcgtcc agatagcggc
     5941 ggcactccgc gatggcgcgg ggatcgggaa acttctggct ctcggtcctg atcgcgacgc
     6001 gccgcccgag ctcggcctcg aaccgcccgc catccacgta ctcccgagat cttccgattg
     6061 cgccgctgcg cgtgccgctc gccatgctct cgctccgatc gccgaaaatg atccagtcgg
     6121 cctgtaagcc gggttctgtc tggcgggttg tcccgccgcg acggccattc gtctgggaga
     6181 tggctcgcgc cgtccctcga gcaaccaacc cggatggcga gcctgaatgc gcgccccgga
     6241 gccgaggttt cccccgctcc cggccatccc tatttggtct tgctcccggt ggggtttgcc
     6301 ctgccgcccc tgttgccagg cgcgcggtgc gctcttaccg caccgtttca cccttgccgg
     6361 acgccccttg cggcggcgga cggcggtttg atttctgtgg cactgtccct gaggtcgccc
     6421 tcggc
//
LOCUS       DAORNH010000271         6412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8965_length_6412_cov_2.706623, whole genome shotgun sequence.
ACCESSION   DAORNH010000271 DAORNH010000000
VERSION     DAORNH010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8965_length_6412_cov_2.706623"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="PK264_20540"
     CDS             complement(<1..1827)
                     /locus_tag="PK264_20540"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HRD78291.1"
                     /translation="MTVQRGSESRTRIAVASQGDRTRAFRRARVNTWFVRFVKIGLPL
                     IAVIALAVYGGRLAGSMSIKSGPVSVSGITIDPENVVVDRPTYDGFAKDGSRYQIRAK
                     SAAGDLRQTGPIKLTDITGDIFQPNGVVTRLTAVRGLYDSKKSELELFEAITIAGSNG
                     LKARLTHAMIWNKEGRIVSKEPVVAELPTATINARSMEAFQKKREANFAGDVHVRMFP
                     AAKAPSPDLKGAADAAAAVAVAKTPRGPGLPGTAMKSDAPIDVWSETLAVNDDAKTAL
                     FKTKVRAVQDAATLESQELEVHYTGKASLDSAPAGTKPKDETAGEAAKLKLLLGRGGV
                     TMVSGPESRATSDDLSYDAESQIAILIGRVVMTSGADKRAVAARAEMDQLKDTALLVG
                     GVHVVQSRNELWGERLMVDRKAGTMHLDSPAAPGKAAGRIKAHLVQTETPEAAARKAA
                     AAAAKPATEAATASPLGVTFRTNPNAPIDIDADTLDANDKSKTAIFRGNVVSRQDEFV
                     MKTPEMIAHYAGESGLASAGLVPSVPKKKGEGAQLTKVEARTKVFVTSKGGQTANGDW
                     AIYDVKTNTVIVGGNVVVTRGKDVVQGQRLKIDLTTGMYRFEL"
     gene            complement(1829..2152)
                     /locus_tag="PK264_20545"
     CDS             complement(1829..2152)
                     /locus_tag="PK264_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78292.1"
                     /translation="MENKALAHHDWEIIRDSASYVLAQVMFNLANASARSPTSGRIDA
                     AVGNVGAARHWCVEVSPSASASCYQLGRSAYRAIVLPRGAGTQCACAARRADFGRADM
                     EMPPD"
     gene            2236..2907
                     /locus_tag="PK264_20550"
     CDS             2236..2907
                     /locus_tag="PK264_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD78293.1"
                     /translation="MINVRRQLLKVSGLLGAFVVAATVAVMPENAEAQRATPRPEVPV
                     TELMKPGPLPDLVYGKADAPVTIVEYASMTCSACGRFHAEVLPKLKAKYIDTGKARLV
                     IRPFARDNLDAAAWLLTHCTMGERSYVLAGALFDRQQQWAFTPNPLPELLKIGKQAGF
                     TEQAFNACVQDAKKLGDITAIRDLAFNNFGVDSTPAFFINGKRFPGFTLEQFEKAIDP
                     LLPKS"
     gene            2911..3636
                     /locus_tag="PK264_20555"
     CDS             2911..3636
                     /locus_tag="PK264_20555"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRD78294.1"
                     /translation="MPILPPRGTGSGGKGSGGIDRRLMIGAAIAVAGLAAVQLYPRLT
                     STSPRASVLGDRVSLDQLMAPGPLPDLMWGKPEAPVTIVEFASMTCGHCGEFHKLVWP
                     KLEEKYIRTGKARLILREYPIDQLALFAAMLGRCAGPEKGRELIGEMFKAQSTWAVGE
                     TGKPAAELTKLWEAQGLPKAEFQACAEKTDLMGQLTAARSRAKSVFAIKGTPTFFVNG
                     YRVTGPTDTIDTFDAAFAEIAKR"
     gene            3638..4402
                     /locus_tag="PK264_20560"
     CDS             3638..4402
                     /locus_tag="PK264_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD78295.1"
                     /translation="MLLIARSLVFVVLAMLTGCSGAALPTGSVASDTASGIAGVSTDK
                     PAFNPFNDPTLSVPGGREVIEKPTLADVMLTGTLPDMSLGRTEAPVTIIKYMSLTCPY
                     CRKFQLEVFPVLKRDYIDKGKVRFILREFPIGHQSGQATVALRCAGQQRFFPLYEKFL
                     TQQVKWVSQEVRLEPIFEIARQVGMSRTEFDSCFKNQGMVDGLKWVKERGRKLGVIGT
                     PNFFINGRLVKSVLGLKELKEIIDPLIAGGAPAPRT"
     gene            4470..>6412
                     /locus_tag="PK264_20565"
     CDS             4470..>6412
                     /locus_tag="PK264_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD78296.1"
                     /translation="MHITKIRLLGFKSFVEPTELLISKGLTGVVGPNGCGKSNLLEAL
                     RWVMGETSHKSMRGSSMDDVIFSGTSTRPERTSAEVTIFLDNTARRAPAEHNGADVIE
                     ITRRIEREAGSAYRINGRDVRARDVKILFEDAATGARSPALVRQGQISELVNAKPEQR
                     RRVLEDAAGIAGLHSRRHEAELRLKAAEANMARIGDVIGQVQSQMESLKRQARQAKRY
                     KELSTELRRAEALLLHLLWHTAQSEVDAQEALLASTLIRLAEVTERESASLREEGETA
                     EQIQPLREDEAGKGAAVHRITIEQETFEKEAERLAHRQQELEGRQGQLQRDLQREEAQ
                     VGEAKELLTKLEGDRTALVEQEAASELAEETARAKLAESETHLQQKEARLAEATRLAF
                     EHRARMKSLEAAAVDRKAAIERLKRQVTTLEQQTREIALKAPDASKLRTLEENGQRLS
                     AALVDIERQATTAEEKVAEAAAVARERRDASQLGQIAASQLRTEMETLTKLLGPIRSD
                     GPAGVIDLLRVAPGYEIALGAALGEDLDAPIAPEAPLHWRRIGMGDGDAALPADVEPL
                     IKHVEGPSELVRRLRQIGVVTRDEGPLLQPELLPGQRLVSLEGDLWRWDGYTATANAP
                     TAAALRLAERNRLGDIVRRESEAR"
BASE COUNT         1083 a         2142 c         2125 g         1062 t
ORIGIN      
        1 gagttcgaaa cggtacatgc ccgtcgtcag gtcgatcttg aggcgctggc cctgcacgac
       61 gtccttgccg cgcgtgacga cgacgttgcc gccgacaatc accgtgttcg ttttcacgtc
      121 gtagatcgcc cagtcgccat tagccgtctg gccgcccttg gaggtgacga acaccttggt
      181 gcgagcctcg accttggtga gctgcgcgcc ctcgcccttc ttcttcggca cgcttgggac
      241 aagccccgcg ctggcgaggc cggactcgcc cgcataatgg gcgatcatct ccggcgtctt
      301 catgacgaac tcgtcctggc gcgagacgac gttgccgcgg aagatcgcgg ttttcgactt
      361 gtcgttggcg tcgagcgtgt cggcgtcgat gtcgatcggt gcgttgggat tggtgcggaa
      421 ggtgacaccg agcgggctcg cggttgcggc ctcagtcgcg ggctttgcgg cagcagccgc
      481 tgctttccgt gctgcggcct cgggcgtctc ggtctgcacg aggtgcgcct tgatccgccc
      541 tgcggccttg cccggtgccg cagggctatc gagatgcatc gtccctgcct tgcgatcgac
      601 catcagccgc tcgccccaga gctcgttgcg cgactggacg acgtgcactc caccgacgag
      661 cagggccgtg tccttcagct gatccatctc ggcgcgtgcg gcgaccgcgc gcttgtccgc
      721 gcccgacgtc atcacgacac ggccgatcag gatggcgatc tggctttcgg cgtcataact
      781 cagatcatcg ctggtcgcgc ggctttccgg tcccgagacc atcgtgacgc cgccgcggcc
      841 gagcagcagc ttgagcttgg cggcctctcc cgccgtctcg tccttcggct tggtaccggc
      901 cggcgcgctg tcgagcgagg ctttgcccgt gtaatgaacc tccaactcct gcgactcgag
      961 cgtcgcggca tcctgcacgg cgcggacttt cgtcttgaag agcgccgtct tggcgtcgtc
     1021 gttcacggcg agcgtctccg accagacatc gatcggcgcg tccgacttca ttgccgtgcc
     1081 aggaaggcct ggtccgcgcg gcgtcttggc gacagccacc gcagcagccg cgtccgcggc
     1141 acccttgagg tcgggtgacg gcgccttcgc agcgggaaac atgcggacat gcacatcgcc
     1201 cgcgaagttg gcctcgcgct ttttctgaaa ggcttccatg gagcgggcat tgatcgtcgc
     1261 ggtcggcagc tcggcaacga ccggctcctt ggagacgatg cggccctcct tgttccagat
     1321 catcgcatgg gtgaggcgtg ccttgaggcc gttcgagccc gcgatcgtga tcgcctcgaa
     1381 cagctcgagc tcgctcttct tgctgtcgta gaggccgcgc accgcagtga ggcgcgtcac
     1441 cacgccgttc ggctgaaaga tgtcgccggt gatgtccgtg agcttgatcg gcccggtctg
     1501 acgaagatcg cccgccgccg attttgcccg gatctgatag cgcgagccgt ccttggcgaa
     1561 gccgtcgtaa gtcggccgat cgacgacgac gttctcggga tcgatggtga tgccggagac
     1621 gctgacgggg cccgacttga tgctcatgga cccagcaagg cgcccgccgt agacggcgag
     1681 cgcgatgacc gcgatcaggg gcaagccgat cttcacgaag cgcacgaacc aggtgttcac
     1741 acgtgcgcgg cgaaaggcac gcgtgcggtc gccctggctt gcgaccgcga tcctggtgcg
     1801 gctctctgat cctcgttgta ctgtcatgtc aatccggcgg catttccatg tcggcgcgtc
     1861 caaaatcggc acgtctggca gcgcaagcgc actgcgtgcc tgcgccgcgc ggcaaaacaa
     1921 tggctcgata cgcggagcgg cccaactggt agcatgaagc ggaagccgac ggcgagacct
     1981 ccacgcacca gtgtcgggcc gcgccgacat tgcccacagc tgcgtcgatc cggccactag
     2041 tcggcgatcg agcactggca tttgccaagt tgaacatgac ctgcgccaag acgtagctgg
     2101 cgctgtcgcg aattatctcc caatcgtgat gcgcgagggc cttattctcc atggcactcc
     2161 tgtgctagga gcctcgtgac tgcggtgcct gcgcggcgtc gcatgccttg gtgtgcctcc
     2221 tggagagctg gtcttatgat caacgttcgt cggcaacttc tgaaagtatc cggactgttg
     2281 ggcgcgttcg tcgtcgccgc gactgttgcc gtcatgcccg aaaacgccga ggcacagcgc
     2341 gcgacaccgc gaccggaggt gccggtcacc gagctcatga agccgggtcc gctccctgat
     2401 ctcgtctacg gcaaggctga tgctcccgtg acgatcgtcg agtatgcctc gatgacctgc
     2461 agcgcctgcg gacgcttcca tgccgaggtg ctgccgaagc tcaaggccaa gtacatcgac
     2521 actggcaagg cgcggctcgt cattcgcccg ttcgcacgcg acaacctgga cgccgccgcc
     2581 tggctactca cgcattgcac gatgggcgag cgcagctatg tcctcgcagg tgcccttttc
     2641 gatcgccagc agcagtgggc attcacgccc aatccgctgc ctgagctcct gaagatcggc
     2701 aagcaggctg gctttaccga gcaggccttc aacgcctgcg tgcaggacgc gaagaagctc
     2761 ggcgacatca cggcgatccg cgatctcgcc ttcaacaact tcggcgtcga ttcgacgccg
     2821 gccttcttca tcaacggaaa gcggttcccg ggtttcacgc tcgagcagtt cgagaaggcc
     2881 atcgacccgc tgctgccgaa gagctgaccc atgccgatac ttccacctcg cggcacgggt
     2941 tcaggcggca aaggctccgg cggaatcgat cgccggctga tgatcggcgc cgcaatcgcg
     3001 gtcgccggcc tcgctgctgt ccagctctac ccgcggctca cctcgacgag cccacgcgcg
     3061 tccgtgctcg gcgaccgggt ctcgctcgac cagctgatgg cgcctggtcc gctcccggac
     3121 ttgatgtggg ggaagcccga agctccggtg acgattgtcg agttcgcctc gatgacctgc
     3181 gggcactgcg gcgagttcca caagctcgtc tggccgaagc tcgaggagaa gtacatccgc
     3241 accggcaagg cgcggctgat cctgcgcgag tatccgatcg atcagctggc actcttcgcc
     3301 gcgatgcttg gccgctgcgc cggccccgag aaggggcgcg agctcatcgg cgagatgttc
     3361 aaggcgcaga gcacctgggc cgtgggcgag accggcaagc ctgccgccga gctgacgaag
     3421 ctgtgggagg cccaggggct gcccaaagcc gagttccagg cctgcgccga gaagaccgac
     3481 ctcatgggcc aactcaccgc ggcgcgcagc cgcgccaaga gtgtcttcgc gatcaagggt
     3541 acgccgacct tcttcgtgaa cgggtatcgt gtcacggggc cgacggacac gatcgacacc
     3601 ttcgatgcgg catttgccga gattgccaag aggtaggatg ctgctgatcg cgcgatcgct
     3661 ggtcttcgtc gtgctcgcca tgctgacggg ttgctccggc gcggctcttc ccacgggcag
     3721 cgtcgcgagc gatacggctt cgggcatcgc cggagtgtcg accgacaagc cggcattcaa
     3781 cccgttcaac gacccgacgc tctctgtgcc gggcggccgc gaggtcatcg agaagccgac
     3841 gctcgccgac gtcatgctga cgggcacgct ccccgacatg tcgctcgggc ggaccgaagc
     3901 gccggtgacg atcatcaagt acatgtcgct cacctgcccc tactgccgga agttccagct
     3961 cgaggtcttc ccggtcctga agcgcgacta catcgacaag ggcaaggtgc gctttatcct
     4021 gcgggagttt ccgatcgggc atcagtccgg tcaggcgacc gtcgcgctgc gctgcgccgg
     4081 ccagcagcgc ttcttcccgc tctacgagaa gttcctcacg caacaggtca agtgggtgag
     4141 ccaggaggtt cgcctcgagc cgatcttcga gatcgcccga caggtgggga tgagtcgcac
     4201 cgaatttgac agctgcttca agaatcaggg catggtcgat ggcttgaagt gggtgaagga
     4261 gcgtggccgc aagctcggcg tgatcggaac tcccaacttc ttcatcaatg gccggctggt
     4321 gaagtcggtt ctcggattga aggagctgaa ggagatcatc gacccgctga ttgcgggcgg
     4381 agcgcccgca ccgcgaacct gatcccgctt ccggtgcccc tgggccgcag cgcggctcgt
     4441 gcgccgaagt ccgatcggag aggccgctcg tgcacataac caagatccgt ctcctcggct
     4501 tcaagtcgtt cgtcgagccg accgagttgc tgatatcgaa gggcctgacc ggcgtggtcg
     4561 gaccgaatgg ctgcggcaag tcgaacctgc tcgaggcgct ccgctgggtg atgggagaga
     4621 cctcgcacaa gagcatgcgc ggctcgtcga tggacgacgt gatcttctca ggcacctcga
     4681 cccggcccga gcgcacctcc gccgaggtta cgatcttcct cgacaatacg gcccgccgcg
     4741 caccggccga gcacaacggc gccgacgtca tcgaaatcac acggaggatc gagcgcgagg
     4801 cgggctccgc ctatcgcatc aacggccgcg acgtgcgtgc gcgcgacgtg aagatcctgt
     4861 tcgaggatgc agcgaccggc gcgcgctcgc cggcgctcgt gcgccaaggc cagatctccg
     4921 agctcgtcaa cgcgaaaccc gagcagcgcc gccgcgtgct cgaggatgcg gccggcatcg
     4981 ccggcctgca cagccgccgc cacgaggcgg agctgcggct caaggctgcc gaagcgaaca
     5041 tggcacgcat cggcgacgtg atcggccagg tgcaatcgca gatggagagc ctcaagcggc
     5101 aggcccgcca ggccaagcgc tacaaggagc tctcgaccga gctccggcgc gctgaggcgc
     5161 tgctgctcca tcttctctgg cacacggcgc aaagcgaggt cgacgcgcag gaggcgctgc
     5221 tggcgagcac gctgatccgc ctcgccgagg tcacggaacg cgagtcggcg tcgctccgcg
     5281 aggaaggcga gacggccgag cagatccagc cgctgcgcga ggacgaggcc ggcaagggcg
     5341 cggcggtgca tcgcatcacc atcgagcagg agaccttcga gaaggaggcc gagcggctcg
     5401 cccatcgcca gcaggagctc gaagggcgcc agggccagct gcaacgcgac ctgcagcgtg
     5461 aagaagccca ggtcggcgag gcgaaggagc tgctcaccaa gctcgagggc gaccggacag
     5521 cgctcgtcga gcaggaggcc gcctcagagc tcgccgagga aacagcccgc gcgaagctcg
     5581 cagaatcgga gacgcacctc cagcagaagg aggcgaggct cgccgaggcg acgcgcctcg
     5641 ccttcgagca tcgcgcgcgc atgaagagcc tcgaggccgc cgcggtcgac cggaaggcgg
     5701 cgatcgagcg gctgaagcgg caggtcacga cgctcgagca gcagacgcgc gagatcgctc
     5761 tcaaggcgcc ggacgcctcg aagctgcgca cgctcgagga gaacggccag cgtctctccg
     5821 cagccctcgt tgacatcgag cggcaggcca ccaccgccga agagaaggtc gcggaagcgg
     5881 ctgcggttgc ccgcgagcgg cgcgatgcct cgcagctcgg ccagatcgca gcgagccaac
     5941 tccgcaccga gatggagacg ctgacaaagc tgctcggccc gatccgcagc gatggcccgg
     6001 ccggcgtcat cgatctcctg cgcgtcgcgc ccggttacga gatcgcgctc ggagcggcac
     6061 tcggcgagga tctcgacgcg ccgatcgcgc cggaagcccc gctgcactgg cgccgcatcg
     6121 gcatgggcga cggcgatgcg gcgctgccgg ccgacgtcga gccgctcatc aagcatgtcg
     6181 aaggaccgtc ggagctcgta aggcgtctgc gccagatcgg tgtcgttacg cgcgatgagg
     6241 gccccctgtt gcagcccgag ctgctgccgg ggcagcgcct tgtctcgctc gaaggcgatc
     6301 tctggcggtg ggacggctac acggcgaccg cgaatgcgcc gaccgccgcc gccctcaggc
     6361 tcgccgagcg caaccgcctc ggcgacatcg tacggcgcga gagcgaggcg cg
//
LOCUS       DAORNH010000272         6408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_8971_length_6408_cov_2.251535, whole genome shotgun sequence.
ACCESSION   DAORNH010000272 DAORNH010000000
VERSION     DAORNH010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8971_length_6408_cov_2.251535"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>344)
                     /locus_tag="PK264_20570"
     CDS             complement(<1..>344)
                     /locus_tag="PK264_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRD78297.1"
                     /translation="KPLIYALAFELGILHPETLIEDRPARFGSYSPKNFDEEFHGTVT
                     IREALAASLNIPAVKVIERIGPQRVTAALRRAGLTPHLPGSGEPSLAIALGGIGLKLH
                     DLAQLFAAIARG"
     assembly_gap    345..354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<355..1403)
                     /locus_tag="PK264_20575"
     CDS             complement(<355..1403)
                     /locus_tag="PK264_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRD78298.1"
                     /translation="MRGGWPRRLKRLLIAMLIGIAISAAAFEVGLRIAFAHLGELPLA
                     ATEETSVAVLDREDRLLRAFTTADGRWRLPVEVGDVDPRYLAMLIAFEDKRFWSHPGV
                     DPMAMTRAAGQLVRHGRIVSGGSTITMQVARLIDGPKSRSATTKFWQMVRALQLERRY
                     SKADILRLYLRLAPFGGNIEGTRAASLAYFGKEPRHLSIGEAALLVALPQSPRLRRPD
                     RHATLARSARDRVLVRARHAGVVSAEDHERSKGEPVPDLRLEFPKLAAHLAEAERAHA
                     PRTSVHRLTLDARLQRDLERLARDHVRPMGPRLSAAILALDHTTGEVLAHVGSAGYLD
                     DDRFGAIDMTRAVRSP"
     gene            complement(1406..>2190)
                     /locus_tag="PK264_20580"
     CDS             complement(1406..>2190)
                     /locus_tag="PK264_20580"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRD78299.1"
                     /translation="VPRLMTLVSKVFSSRTYTSTQEQAWLLLAARALAEDARAMQLMV
                     GTTPHRGPLSRGIGLDELAAGPLVIENQGDGEVASLLSVRGASLTAEPAVERGFSIER
                     SYYTLDGQKVELKSATGGTSSVKQNDRLVVVVKVTLKEKEKGGRILLVDRLPAGLEIE
                     NARLVDSGSVKSLDWLKRTVEPTHTEFRDDRFVAAFDVFRRGRRSEEGDAKAPDTLVV
                     AYIVRAVTPGTFTHPAATVEDMYQPERLARTAAGRLTVTEGK"
     assembly_gap    2191..2200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2201..>2801)
                     /locus_tag="PK264_20585"
     CDS             complement(<2201..>2801)
                     /locus_tag="PK264_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRD78300.1"
                     /translation="SRALPLLYSAEMARRVGITSDAEIKKRVQTAIDRLAEMQDSSGA
                     FGLWGPGSPDLWLTSYVTDFLTRAKEAGDEVRAEVISQALDRLANYAANVTTFDGGGE
                     SRAYAHYVLARSGRGQVGELRYLVDSRLERFVTALAQAHLGAALSMVGDKERAERVIR
                     AAILKLDGTAPEVTRLDYGSRLRDGAAVVTLIAETGVART"
     assembly_gap    2802..3033
                     /estimated_length=232
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3034..>6408)
                     /locus_tag="PK264_20590"
     CDS             complement(<3034..>6408)
                     /locus_tag="PK264_20590"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRD78301.1"
                     /translation="SDGEAWLLFARAQLAVRPDPMMPQDAPQRRARASGAALIATQKL
                     KAKPRQAEAFGVLADSLRVRSMWKPAIDALKVSVSLASDAERKRQLEALRAEHGFRVV
                     DYKVETTGTEPRVCLTLSEKLGMTDVAKFIAVDGRDPQAASGDDQQICIDGFDYGKRY
                     TVTVKADLPAESGDSLVRTAELQIAIRDRSPSVRFSGRAYVLPRRGQTGIPVVSINVE
                     KVAVEVYRVGDRMLAPTLQSGDFLKQLDGEQIEQIRTKSGEKVYSGTLEVKPKTNEEV
                     TTAVPIGEAVPTMKPGIYVMIASRIGDKAESYDLKATQWFVVSDIGLTALTGEDGIHA
                     FVRSLASTNPMSGARVRLVAKNNELLGEGRADANGYVHFAAGLKRGEGGMAPAVLVAE
                     SGEGDYAFLDLTSAAFDLSDRGVSGREVPGPVDAFLYAERGVYRPGEEVNLTALVRDR
                     AGRANAVPVTLVITRPDGVEARRTTLLDRGLGARTHIHSLTGSSMTGTWRARIYTDPK
                     AAAIAQLAFLVEDFVPERVELKLDADGVTLVPGGEERDIKVAGRYLYGPPAAGLTIEG
                     DIVVKPASGDLPGFPGFRFGNALEKVAPVRSQLEDLPNTDESGNAVVAIKLPAVPKTV
                     RPLEADVMIRLREAGGRAIERKVTLPVLTGKPRVGIKGLFTGAGVDEGAPASFEAVLI
                     DGENKPVAAKSMKWELFALDQRWQWFSREGSWQFEAITTSRRIGNGIANATAGAERVR
                     IDVPRLDWGRYRLDVSTGEPGGAVSSFVFNAGSYSADGPDNPETLDVALDKPSYKAGE
                     TAKVRIASRFAGRAQIAVISNGLLAQKIVDVAAGGSEATLDVAESWLPGAYVAVTLFR
                     PMDETARRMPARAVGIRWLGMDTSSKRLEVSLAAPAKTKPGTRLSVPVKIGGLASGEK
                     AMLTLAAIDAGILGVTRYEPPRPEAHFHGQRRLGVEMRDLYGRLIDGMRAERGRLRSG
                     GDGDTPAKANANPPVEKLLALFSGLVTVGADGNAVVDFDLPEFNGTVRLMAVAWSEGK
                     VGSATSDVVVRDAVALTIAAPRFMMLGDQTRLLLDIHNVEGPAGQYAVTVQRRVATKA
                     PAAAPVMLDLKSGQRATHTVRLEPREIGESVYDVRIA"
BASE COUNT          931 a         2101 c         2163 g          961 t
ORIGIN      
        1 cgccccgcgc gatcgcggcg aagagctgcg cgaggtcgtg gagcttcaag ccgatgccgc
       61 cgagcgcaat ggcgagcgag ggctcgccgc ttcccggaag atgcggcgtg aggccggcgc
      121 ggcgcagcgc cgccgtgacg cgctgcggcc cgatgcgctc gatcactttg acggccggga
      181 tattgagtga ggcggcgagc gcctcgcgga tcgtcaccgt gccgtggaac tcctcgtcga
      241 agttcttcgg gctgtagctg ccgaagcgcg ccggccggtc ctcgatcagc gtctcggggt
      301 ggaggatgcc gagctcgaag gcgagcgcgt aaatgagcgg cttcnnnnnn nnnnggcgag
      361 cgcaccgcgc gcgtcatgtc gattgcgccg aagcggtcgt cgtcgaggta gccggccgag
      421 ccgacgtggg cgaggacctc tcccgtcgtg tggtcgagcg ccaggatggc ggcggacaac
      481 cgcgggccca tcgggcggac gtgatcacgg gcgagccgct cgaggtcgcg ctgaaggcgg
      541 gcatcgagcg tcagacgatg cacgctcgtt cgcggtgcgt gagcccgctc ggcttcggca
      601 aggtgagccg ctagcttcgg gaactcgagg cggaggtccg gcaccggctc gcccttcgag
      661 cgctcgtgat cctccgccga gacgacgccc gcatggcgcg cccgcacgag cacccgatca
      721 cgggcgctgc gggcgagcgt cgcatggcga tccgggcggc gcagccgcgg cgactgcggc
      781 agtgcgacca acagtgccgc ctcgccgatc gagagatgcc gcggctcctt gccgaagtac
      841 gcgagtgaag ccgcccgagt tccctcgatg ttgcctccga agggcgcgag ccgcaggtag
      901 agacgtagga tgtcggcctt ggagtagcgc cgctcgagct gcagcgctcg caccatctgc
      961 cagaacttgg tcgtggccga gcgcgacttc ggcccgtcga tgagacgtgc gacctgcatc
     1021 gtgatggtcg agccgccgga aacgatgcgt ccatgccgga cgagctgccc cgccgcgcgc
     1081 gtcatcgcca ttgggtcgac acctgggtgc gaccagaagc gcttgtcctc gaaggcaatc
     1141 agcatcgcga ggtagcgcgg atcgacgtcg ccgacctcga cgggaagccg ccagcgcccg
     1201 tcagctgtcg tgaaggcgcg gagcagccgg tcctcgcgat cgagaacggc gacgctggtt
     1261 tcttcggttg ccgcgagcgg cagctcgccg aggtgcgcaa aggcgatgcg cagaccgacc
     1321 tcgaacgcag cggcggagat cgcaatgccg atcagcatcg cgatcagaag ccgcttgagg
     1381 cgcctcggcc agcccccgcg catgcctact tgccctcggt cacggtaagg cgaccggcgg
     1441 cggtgcgcgc gaggcgctcg ggctggtaca tgtcctcgac cgtcgccgcc gggtgcgtga
     1501 acgtgcccgg cgtcacggcg cggacgatat atgcgacgac gagcgtatcc ggcgccttgg
     1561 cgtcgccctc ctcgctgcgg cggccgcggc ggaacacatc gaacgcggcg acgaagcggt
     1621 cgtcgcggaa ctcggtgtgc gtcggctcga ccgtacgctt cagccagtcg agcgacttga
     1681 cgctgccgct gtcgacgagg cgcgcgttct cgatctcgag gcccgccggc aggcgatcga
     1741 cgagcaggat gcggccgccc ttctcctttt ccttgagcgt cactttaacg accacgacga
     1801 gccggtcgtt ctgcttgacg ctcgaggtgc cgccggtggc gctcttcagc tccaccttct
     1861 gcccatcgag cgtgtagtag ctgcgctcga tcgagaagcc gcgctcgacc gcgggctccg
     1921 ccgtcaacga agcgccgcgc acggagagca gcgaggccac ctcgccgtcg ccctggttct
     1981 cgatgacgag cggaccggcc gcaagctcat cgaggccgat gccgcgactg agcggaccgc
     2041 ggtgcggcgt cgtcccgacc atgagctgca tcgcgcgggc atcctcagcc agcgcccggg
     2101 ccgcgagcag gagccaggcc tgctcctgcg tcgaggtgta ggtgcgactc gaaaagacct
     2161 tggagacgag cgtcatgaga cgcggcacct nnnnnnnnnn gtgcgggcga cgccggtctc
     2221 ggcgatcagg gtcaccacgg cggcaccatc acggagccgc gagccgtagt cgaggcgcgt
     2281 gacctcgggc gccgtgccat cgagcttcag gatcgccgca cggatcaccc gctcggcccg
     2341 ctccttgtcg ccgaccatcg acagggccgc accgagatgc gcctgggcga gcgccgtcac
     2401 gaagcgctcg aggcggctgt cgacgaggta gcgcagctcg ccgacctggc ctcggccact
     2461 gcgcgcgagc acatagtgcg cgtaggcccg gctctcgcca ccgccgtcga aggtcgtcac
     2521 gtttgcggcg tagttggcga gccggtcgag cgcctgcgag atcacctcgg cgcggacctc
     2581 gtcgcccgcc tccttggcac gcgtcaggaa gtcggtgacg tagctcgtca gccacaggtc
     2641 cggcgagccc ggcccccaga gtccgaaggc gcctgaggaa tcctgcatct ccgccagacg
     2701 gtcgatcgcc gtctgcacgc gcttcttgat ttcagcatcg ctggtgatgc cgacgcgacg
     2761 cgccatctcg gctgagtaga gcagcgggag ggcgcggctc cnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggcgatg cgcacatcat agacgctctc
     3061 gccgatctcg cgcggctcga gcctcacggt gtgcgttgcc cgctggccgc tcttcagatc
     3121 gagcatcacc ggggccgcag cgggcgcctt tgtggcaacg cgccgctgca cggtgacggc
     3181 atactgtcca gccggtccct cgacattatg gatgtcgagg aggaggcggg tctgatcacc
     3241 gagcatcatg aagcggggtg ccgcgatcgt gagtgccacg gcatcacgca cgacgacgtc
     3301 ggacgtggcg gagccgacct tgccctcgct ccacgcgacc gccatcaggc ggacggtgcc
     3361 gttgaactcg gggagatcga agtcgacgac ggcgttaccg tcggcgccga ccgtgaccag
     3421 tccggagaaa agcgcgagca gcttctcgac cggcggattg gcattcgcct tcgccggcgt
     3481 gtcgccgtcg ccgccggagc ggagccggcc gcgctcggcg cgcatgccgt cgatcaagcg
     3541 gccgtagaga tcgcgcatct cgacgccgag gcggcgctgg ccgtggaagt gcgcttcggg
     3601 ccgcggcggc tcgtagcggg tgacgccgag gatgccggca tcgatcgcag cgagcgtcag
     3661 catggccttc tcgccggagg cgaggccgcc gatcttcacc ggcacgctga ggcgcgtccc
     3721 gggtttcgtc ttggcgggag cggctaggct cacctcgagc cgcttcgacg aggtgtccat
     3781 gccgagccag cggatgccga ccgcgcgcgc gggcatgcgg cgggccgtct cgtccatggg
     3841 gcggaacagc gtgacggcga cataggcacc aggcagccag ctctcggcga cgtcgagcgt
     3901 cgcctcgctg ccgccggccg ccacgtcgac gatcttctgc gccagaaggc cgttcgagat
     3961 gactgcgatc tgtgcccggc ccgcaaaccg cgaggcgatg cgcaccttcg ccgtctcacc
     4021 ggccttgtag ctcggcttgt cgagggcgac gtcgagcgtc tccggattgt caggaccatc
     4081 ggccgagtag gagcccgcgt tgaagacgaa gctcgacacc gcaccgcctg gctcgccggt
     4141 cgatacatcg aggcggtagc ggccccagtc gagtcgcggc acatcaatcc tgacgcgctc
     4201 ggccccggcc gtggcattgg cgattccgtt gccgatgcgg cggctcgtcg tgatcgcctc
     4261 gaactgccac gagccctcgc ggctgaacca ctgccagcgc tggtcgagcg caaacagctc
     4321 ccacttcatc gacttggcgg cgacgggctt gttctccccg tcgatcagga ccgcctcgaa
     4381 gctcgccggc gcgccctcgt cgaccccggc acccgtgaac aggcccttga tgccgacgcg
     4441 gggcttcccc gtcagcacgg gcagcgtgac cttgcgctcg atggctcgac caccggcttc
     4501 acggaggcgg atcatgacgt cggcctcaag cgggcggacg gtcttcggca cggccggcag
     4561 cttgatggcg acgacggcgt tcccgctctc gtctgtgttc ggcaggtcct cgagctggct
     4621 gcggaccggc gcaacctttt cgagtgcgtt gccgaagcgg aagcccggga agcccggcag
     4681 atcgccgctc gccggcttca cgacgatatc gccctcgatc gtgagacccg cggctggggg
     4741 accgtagagg tagcggccgg ccaccttgat gtcgcgctct tcaccgccag gtacgagcgt
     4801 aacgccgtcg gcatcgagtt tcagctcaac acgctccggc acgaagtcct cgacgaggaa
     4861 ggcgagttgc gcgatcgccg ccgccttcgg gtctgtatag attcgcgccc gccaggtgcc
     4921 cgtcatcgac gagccggtca ggctgtgaat gtgcgtccgc gcaccgaggc cccgatcgag
     4981 cagcgtcgtg cggcgcgcct cgacgccgtc tggccgcgtt atgaccagcg tcacggggac
     5041 ggcattggcg cgaccggcgc gatcacggac gagggctgtg aggttgacct cctcgccggg
     5101 gcgatagacg ccgcgctcgg catagaggaa ggcatcgacc ggtccgggca cctcgcggcc
     5161 ggagacgccg cggtccgaaa ggtcgaaggc ggccgaggtg agatcaagga aggcgtagtc
     5221 gccctcgcca ctctcggcga ccagcacggc gggggccatg ccgccctcgc cccgcttcag
     5281 gccagcggcg aagtgcacgt agccgttggc gtccgcgcgg ccttcgccga gcagctcatt
     5341 gttcttggcg acgaggcgga cacgcgcgcc gctcatcgga ttggtcgagg cgagcgagcg
     5401 cacgaaggcg tggatgccgt cctcgccggt cagtgccgtg agcccgatgt cggagacgac
     5461 gaaccactgc gtggccttga gatcgtagct ctcggccttg tcgccgatgc gggaggcgat
     5521 catcacgtag atcccaggct tcatcgttgg cacggcctcg ccgatcggga ccgcggtcgt
     5581 cacctcctca ttggtcttcg gcttcacttc gagtgtgccc gagtagacct tctcgccgct
     5641 cttcgtcctg atctgctcaa tctgctcgcc gtcgagctgc ttcaggaaat cgccgctctg
     5701 cagcgtcggc gcgagcatgc gatcaccgac ccgatagacc tcgacggcga ccttttcgac
     5761 attgatgctg acgacgggga tgccggtctg accgcgccgc ggcagcacat aggcacggcc
     5821 cgagaagcgc accgatggag aacggtcgcg gatcgcgatc tgcagctcgg ccgtgcggac
     5881 gagggaatcg cctgattcgg ccgggagatc ggccttcacc gtcacggtgt accgcttgcc
     5941 gtagtcgaac ccgtcgatgc agatctgctg gtcatcgccg gacgccgcct gcgggtcacg
     6001 gccgtcgacg gcgatgaact tggcgacgtc cgtcatgccg agtttctcgg agagcgtcag
     6061 gcagacgcgc ggctcggtgc cggtcgtctc gaccttgtag tcgacgacgc ggaagccgtg
     6121 ctcggcgcgc agtgcctcga gttggcgctt ccgttcggca tcactggcga ggctcacgga
     6181 gaccttgagc gcgtcgatcg ccggcttcca catcgagcgc acgcggaggc tgtcggcgag
     6241 cacgccgaag gcctcggcct ggcggggctt cgccttgagc ttttgcgtgg caattagggc
     6301 cgcgccactc gcgcgggcgc ggcgctgggg cgcatcctgc ggcatcatcg gatccggccg
     6361 cacggcgagc tgggcgcgcg cgaacagcag ccaggcctcg ccatcgct
//
LOCUS       DAORNH010000273         6385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9015_length_6385_cov_2.615166, whole genome shotgun sequence.
ACCESSION   DAORNH010000273 DAORNH010000000
VERSION     DAORNH010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9015_length_6385_cov_2.615166"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PK264_20595"
     CDS             <1..932
                     /locus_tag="PK264_20595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRD78302.1"
                     /translation="DKFATFAEYDAFCRAWLGECRRILKPDGAIWVIGSYHNIFRLGV
                     ALQDLGYWLLNDVIWRKTNPMPNFRGKRFTNAHETLIWAARDKQSRPAFNYEALKAGN
                     DDVQMRSDWLFPICSGPERLKDEGGRKAHPTQKPEALLHRILIGTTAPGDVVLDPFLG
                     TGTTAAVAKKLRRHFIGIERDPDYAAAARERISAITPLGHSALDVMASKRAEPRVPFG
                     AIVELGMLRAGSWLTDERGRIRAEVKADGSIAVPGRQGSIHRMGAELQGKAACNGWTY
                     WHYEAEGRLRPIDVLRVEARRRLGLGSADGAEV"
     gene            1009..2469
                     /locus_tag="PK264_20600"
     CDS             1009..2469
                     /locus_tag="PK264_20600"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRD78303.1"
                     /translation="MRLSDLTGPNHVLPADAASVQITGITADSRAVERGFLFAALPGT
                     KVDGARFAMAAKGAGAAAILASERADLPEGLGIPVVRANKPRKALAEMAARFHGRQPA
                     SIVAVTGTNGKTSVAEFTRQIFEALGRRAASLGTLGLVRPGGAAYGSLTTPDPVSLHR
                     SLAALATEEVTHLALEASSHGLDQYRLDGVRLAAAAFTNLGRDHLDYHATVEDYLAAK
                     LRLFRVLLEAGRSAVVNADGAGSERVIDVAHARGLKLITTGVLGETLRLANVRRDGFA
                     QRLEVIYEGRTFELLLPLVGAYQAENALVAAGLAIAVGEEPARAIAAISAIKGVKGRV
                     EIAGTARGGLVVIDYAHKPEALSSVLDALRPFASSRLICVFGCGGDRDKGKRPIMGRI
                     AAEKADITIVTDDNPRSETPSTIRTEVLGGTVGARGEVRDIGGRAEAIGAAIGMLSEG
                     DVLVVAGKGHETGQIVGNDTLPFSDHDVVATFLARV"
     gene            2475..3887
                     /gene="murF"
                     /locus_tag="PK264_20605"
     CDS             2475..3887
                     /gene="murF"
                     /locus_tag="PK264_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRD78304.1"
                     /translation="MPAPLWMFADLATALDGTIDSGGAAPPDVISGLSIDTRSLAPGE
                     VFVALTDQRDGHAFVGDAFRRGARAAVVRRDYARAAGDGTLFRVDDPLAALCRLASAA
                     RARLALDASLIAVTGSAGKTGTKEMLRRALAPFGAVHAPEKSFNNHWGVPLTLARMPA
                     ETRFAVIEIGMNHAGEITPLTGLARPDIAVVTNVLPVHLGQFGSEEGIAEAKAEIFLG
                     LEAHGIAILNGDNRHFERLGERARARGARVVPFGLGATAEWHVQSLVGGESCSDVELA
                     KDGRTIRYRLGAPGRHLAENSLAVATVLDVLGLDVERGLAELAAFRAPAGRGERTEIG
                     VRGGAFLLIDEGYNANPASVAAALAAMSGVPRARFPRRIAVLGDMLELGAASEGLHLA
                     LARTIDEGGVDLVFACGPEMGKLYRSLPERSRGAHRPTSDELAPIVAGAVQPGDVVMI
                     KGSLGTRMAPIVAAVKGLAA"
     gene            3942..5027
                     /gene="mraY"
                     /locus_tag="PK264_20610"
     CDS             3942..5027
                     /gene="mraY"
                     /locus_tag="PK264_20610"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRD78305.1"
                     /translation="MLYELAEFSNQIGPLNVFKYVTFRTGGAIFTALLFVLLFGPGII
                     DLLRLKQGKGQPIRTDGPQSHLAKRGTPTMGGLMILSGVTVSTLLWANWSNPYVWIVL
                     GVTLAFGAIGFYDDYLKVTKQTTSGFGGKARLLLELLVAAAATYVIMRLGKSGFGDAI
                     TFPFFKNLITPSLGFVFVIFGMLVIAGAGNAVNLTDGLDGLAIGPVMIAATAFGLIAY
                     VTGHAKFALYLQIHHVVGTSELAVFCGALLGAGLGFLWFNAPPAMIFMGDTGSLALGG
                     ALGTIAVATKQEVVLAIIGGLFVLEALSVIIQVASFKLTGRRVFRMAPLHHHFEQKGW
                     QESTIVVRFWIVSVVLALIGLTSLKVR"
     gene            5343..6014
                     /locus_tag="PK264_20615"
     CDS             5343..6014
                     /locus_tag="PK264_20615"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD78306.1"
                     /translation="MDALRSGQTGLARRQFELLVARHPESPLAGRARAALREIYAAEA
                     TGAPAVATRHPTESVRPAAPDAAKGWTAKIKPPKAPEADFAATVGDRVFFSEGGAELG
                     GRARLILAAQARWLNDHPAVPLVIEGYADDPGSIEHNASLARRRAEAVRARLVEEGVS
                     AVRIRIVVDDREPRIVTCREADCRHLNRLVVTVVGRSAQPLAAAPPSAGAFPVPMTRL
                     GGPRR"
BASE COUNT          985 a         2147 c         2193 g         1060 t
ORIGIN      
        1 gggacaaatt cgcgaccttc gccgagtacg atgccttctg ccgtgcctgg ctcggcgagt
       61 gcagacgcat cctgaagccg gatggcgcga tctgggtgat cggctcctac cacaacatct
      121 ttcgcctcgg cgtggcgctt caggatctcg gctactggct gctcaacgac gtcatctggc
      181 gcaagaccaa cccgatgccg aattttcgcg ggaagcgctt cactaatgcg cacgagacgc
      241 tgatctgggc ggcgcgcgac aagcaatcac ggccggcatt caactacgag gcgctgaagg
      301 ccggcaacga cgacgtccag atgcgctcgg actggctgtt cccgatctgc tcggggccgg
      361 aacgcctcaa ggacgaggga gggcggaagg cgcatccgac gcagaagccc gaggcactcc
      421 tgcatcgcat cctgatcggc acgacggcgc caggtgacgt agtgctcgac ccgttcctcg
      481 gcaccggcac gacggcggcg gttgcaaaga agctccgccg gcacttcatc ggtatcgagc
      541 gcgatccgga ctatgcagca gctgcgcgag agcggatctc tgccatcacg ccgctcggtc
      601 actcggcact cgatgtgatg gcgagcaagc gtgccgagcc gcgggtgccg ttcggcgcaa
      661 tcgtggagct cggcatgctc cgcgcggggt catggctcac ggacgagcgc ggccgcattc
      721 gcgcggaggt gaaagcagac ggctccatcg ccgtgccggg gcggcagggc tcaatccacc
      781 ggatgggtgc ggaactccag ggcaaggccg cctgcaacgg ctggacctat tggcactacg
      841 aggccgaggg gcggctgcgc ccgatcgatg tgctccgcgt cgaggcgcgc cgccggctcg
      901 ggctcggctc ggccgatggc gccgaggttt gacgatcggc cgggccccgc ataagagggc
      961 agatcgccag cgtcgcggga ttcgccgcag cggcctccgg ggaccccgat gcgcctcagc
     1021 gatttgaccg gaccgaacca tgtgctgccg gctgatgcgg cctcggtcca gatcaccggg
     1081 atcacggccg acagccgtgc ggtggagcgc ggcttcctgt ttgcggcgct gcccgggacc
     1141 aaggtggacg gcgcgcggtt cgcaatggcg gcgaaggggg ctggagctgc agcaatcctc
     1201 gccagcgagc gtgcggacct gcctgagggc ctcggcattc cggtcgtccg tgccaataag
     1261 ccgcggaaag cgttagccga gatggcggct cgcttccatg ggcgacagcc cgcctcgatc
     1321 gtcgcggtca cggggaccaa cggcaagacc tcggtcgccg agttcacgcg ccagatcttc
     1381 gaggcgctcg gccgccgcgc ggcatcgctc gggaccctcg gcctcgtgcg cccgggcggc
     1441 gcggcctacg gctcgctcac cacgcccgat cctgtgagcc tgcacaggtc gctcgccgcg
     1501 cttgcgaccg aggaggtgac gcatctcgcg ctcgaagcct cctcgcacgg cctagaccag
     1561 tacaggctcg acggcgtccg ccttgcggcc gcagctttca cgaacctcgg gcgcgaccac
     1621 ctcgattatc acgcgacggt cgaagactac ctcgcagcca agctgcgtct cttccgggtg
     1681 ctgctcgagg cggggcgcag tgccgtcgtc aatgccgatg gcgcaggatc ggagcgggtg
     1741 atcgatgttg ctcacgcgcg aggcctgaag ctcatcacga caggcgtgct gggagagacg
     1801 ctgaggctcg caaacgtgcg ccgcgacggc ttcgcccagc gcctcgaagt catctacgag
     1861 ggccgcacat tcgagctgct tctgcccctg gtcggcgcct accaggccga gaacgcgctg
     1921 gtcgcggcgg gcctcgcgat cgcggtcggc gaggagcccg cgcgcgcgat tgcggccata
     1981 tcagccatca agggcgtcaa ggggcgtgtc gagatcgcag gcactgcgcg aggcggcctc
     2041 gtcgtcatcg actacgccca caagcccgag gcattgtcat ccgtcctcga tgcgctgcgt
     2101 ccgttcgcga gcagccggct catctgcgtg ttcggctgtg gcggcgaccg tgacaagggg
     2161 aagcgcccga tcatggggcg catcgccgcg gagaaggccg acatcaccat cgtcaccgat
     2221 gacaacccac gctccgagac accgtcgacc atccgcacgg aagtactcgg cggcacggtg
     2281 ggggcgaggg gcgaggtgcg cgatatcggc ggccgggcgg aggcaatcgg ggcggcgatc
     2341 ggcatgctca gtgagggcga tgttctggtc gttgcgggca aaggtcatga gaccggccag
     2401 atcgtcggca atgacacgtt gccgttctcg gaccacgacg tggtcgcaac gttcctcgcg
     2461 agggtctgac gccgatgcct gcgcccctct ggatgttcgc tgatctggca actgcgcttg
     2521 atggaacaat cgattccggc ggggcggcgc ctcccgacgt catctcgggc ctctcgatcg
     2581 acacgcgaag ccttgcgccg ggcgaggtgt tcgtcgccct gaccgatcag cgcgacggcc
     2641 acgccttcgt cggcgatgca tttcgccgcg gcgccagggc ggcggtcgtc cggcgcgatt
     2701 atgcgcgggc ggcgggcgat ggcacgctat tccgtgtcga tgatccgctc gccgcactct
     2761 gccgcctcgc cagcgccgcg cgcgcgcgac tcgcacttga cgcaagcctc atcgccgtca
     2821 cgggcagcgc cggcaagacc ggcaccaagg agatgctgcg ccgggcgcta gccccgttcg
     2881 gcgccgtgca tgcgcccgag aagtcgttca acaatcactg gggcgtacca ctgacgctcg
     2941 cccgcatgcc ggccgagacg cgcttcgcgg tgattgagat cggcatgaac cacgcgggcg
     3001 agatcacgcc gctcacgggg ctcgcacggc ccgacatcgc cgtcgtcacc aacgtgctgc
     3061 ccgtccatct cggccagttc ggctcggagg agggtatcgc ggaggcgaag gctgagatct
     3121 tccttgggct cgaggctcac ggcatcgcga tccttaacgg cgacaaccgg catttcgagc
     3181 ggctcggcga gcgggcgcgt gcgcgtggcg cgcgcgtcgt cccgttcggg ctcggcgcca
     3241 cggccgaatg gcacgtgcag agcctcgttg gtggggaaag ctgcagcgac gtggaacttg
     3301 caaaggatgg ccggacgatc cgctaccgcc tcggcgcgcc cgggcggcat ctcgccgaga
     3361 actcgctcgc cgtcgcaacg gtgctcgacg tgctcggact cgatgtcgag cgcggccttg
     3421 cggagctggc ggcattccgt gcgccggccg ggcgcggcga acggaccgag atcggggtgc
     3481 gcggcggcgc gttcctgctc atcgacgaag gctacaacgc caatccggcc tccgtcgccg
     3541 cagcgcttgc agcgatgtcc ggcgtgccgc gtgccagatt cccgcgccgc atcgccgtgc
     3601 tcggggacat gttggagctc ggcgcggcgt ccgagggact gcacctcgcg ctcgccagaa
     3661 ccattgacga gggcggggtc gatctggtct ttgcctgcgg gccggagatg ggaaagctct
     3721 accggtccct gcccgagcgc tcgcgcggcg cccaccggcc cacatccgac gaattggcac
     3781 cgatcgttgc cggtgcggtc caaccaggtg acgtcgtgat gatcaagggc tcgctcggaa
     3841 cgcgcatggc gccgatcgtc gcggccgtga aagggctcgc tgcgtgaacg gtgtggcgga
     3901 cgtaggacgg tcgatcggcg aggcacacgg ggagagggtg aatgctctat gagctcgccg
     3961 aattcagcaa ccagatcggg ccgctgaacg tcttcaagta cgtcaccttc cgcaccggcg
     4021 gcgcgatctt cacggcgctt ctcttcgtgc tgctgttcgg gccgggcatc atcgatctcc
     4081 tgcgcctgaa gcagggcaag ggccagccga tccgcaccga cggcccccag agccatctcg
     4141 ccaagcgcgg cacgccgacc atgggagggc tcatgatcct ttccggcgtc acggtctcga
     4201 cgctgctctg ggcgaactgg tcgaacccgt acgtgtggat cgtgctcggt gtcacgctcg
     4261 ccttcggggc gatcggcttc tacgacgact acctgaaggt caccaagcag acgacctcgg
     4321 gcttcggcgg caaggcgcgc ctgctgctcg agctgcttgt cgcggcggcg gcgacctacg
     4381 tcatcatgcg gctcggcaag tctggcttcg gtgatgcgat cacattcccg ttcttcaaga
     4441 acctgatcac gccgtcgctc ggcttcgtgt tcgtcatctt cggcatgctg gtgatcgccg
     4501 gcgcgggcaa tgccgtgaat ttgaccgacg gcctcgatgg ccttgccatc ggccccgtga
     4561 tgatcgcggc gaccgccttc ggcctcatcg cctacgtgac tggccacgcg aagttcgcgc
     4621 tttacctgca gatccaccac gtggtcggca cgagcgaact cgccgtgttc tgtggggcgc
     4681 tactcggagc cgggctgggc ttcctgtggt tcaacgctcc tccggccatg atcttcatgg
     4741 gggatacggg ttcgctcgcg ctcggcggcg ccctcggcac gatcgcggtc gcgaccaagc
     4801 aggaggtcgt gctggcgatt atcggcgggc tcttcgtgct cgaggccctc tccgtgatca
     4861 tccaagtggc ctcgttcaag ctgaccgggc ggcgcgtctt ccgcatggcg ccgctgcacc
     4921 accatttcga gcagaagggc tggcaggaga gcacgatcgt cgtgcgcttc tggatcgtct
     4981 cggtggtgct ggccctgatt ggcctcacgt cgctcaaggt gcgctgagac ggcgccgcac
     5041 aatggccggt ctgctgccgc gccctgccaa cttcggcccc aatgttgagg caatctcctc
     5101 cttgctgagc tgcgaatgac tgtggagctg tgggagattg cacgatgcgc gcctcgattc
     5161 cgtcgcgctc tgtgacggcc ggatggcgga gcgggttctt ggtcacggcc acgcttggag
     5221 cgattttggc cgggccggga ggcgcaagtg cgcagacgcc gtggagcact gtcccggatt
     5281 cgggccctat cgcaactccc cccgccgcag ttgacaccga aacgctcgtc ttccgtcgtg
     5341 ccatggacgc cctgagaagc gggcagaccg ggctcgcccg ccgccagttc gaactgctcg
     5401 tcgcgcgcca ccccgaaagc ccgctcgcag gacgggcgcg cgcagctctg cgtgagatct
     5461 acgcggccga ggcgacagga gcacctgccg tggccacgcg acatccaacc gagtcggtaa
     5521 ggcccgccgc gcccgatgcc gccaagggat ggacggccaa gatcaagcca cccaaggctc
     5581 ccgaagccga ctttgcggca acggtcgggg accgggtctt tttcagcgaa ggcggcgcag
     5641 agctcggcgg tcgcgctcgg ctcattctgg cggcgcaggc gcgctggctc aacgatcatc
     5701 ccgccgttcc cctagtcatc gaaggctacg ccgacgatcc gggcagcatc gagcacaatg
     5761 cgagcctcgc ccggcgacga gccgaggccg tgcgggcgcg cctcgtcgag gaaggggtct
     5821 ccgcagtccg aattcgcatc gtcgtcgatg atcgcgagcc gcggatcgtc acctgtcgcg
     5881 aggccgactg ccgccatctc aatcggctgg tcgtgacggt ggtcggtcgg agcgcccaac
     5941 cgctcgccgc tgcgccacct tctgccggcg ctttcccggt gccgatgacg cgtctcggcg
     6001 ggccgcgccg ttgacaagtc gtgccggctg gagacagttc agcactgccg gatcgcgaat
     6061 cgccatcgtt acgcccggaa tcgccggcat tggcatggcg gcggcgctat gatcgacttg
     6121 tgcacgacgt cgcgatgaat tttgttctcg cacggactgc gcagtacttt ccccgatgcg
     6181 gccagtcgcg ggggcggcgg ttctgtccga tttgagaccg atgtactgaa gagcttgacc
     6241 cgaagagaga gtgccatgac cacgagagcg ctgtcatcga aggtgctcgg ggccgcgctg
     6301 gccctttcga tcgtcgcgga gttcgtgccc ctcggcggcg aggccgcggc tcagggcacg
     6361 ccctggccac cgccggcgcc gcgct
//
LOCUS       DAORNH010000274         6316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9145_length_6316_cov_2.042645, whole genome shotgun sequence.
ACCESSION   DAORNH010000274 DAORNH010000000
VERSION     DAORNH010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9145_length_6316_cov_2.042645"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK264_20620"
     CDS             complement(<1..724)
                     /locus_tag="PK264_20620"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HRD78307.1"
                     /translation="MSTFHPLVDHRFSRRTVLQSLSAGFGWLALAGLAQGGTRHHRAR
                     ARSIIFCFMDGGPSHVDTFDPKPMLKEHEGKPIGESAVSNLSQSSANRVWLGCPWEFR
                     QRGESGLWVSDLFPRIAQVADELCVVRSMTGELPLHGQQNLLLHTGRILGQAPSMGAW
                     VNYGLGTENENLPGYVVLNNDWVPNGGLENFGSSYLPASHQASMIRANGVPLDNIVPA
                     LETPQQRRLLELIGQQDRAFAAS"
     gene            complement(730..>1244)
                     /locus_tag="PK264_20625"
     CDS             complement(730..>1244)
                     /locus_tag="PK264_20625"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRD78308.1"
                     /translation="LLAVSGQLDPSLHGRPIRPHRPAEDPQKRLFSGPLDGDRRRSLY
                     LEMSVMQPPEFLVGFNLPDLKQPNGARDVTNVPAQALILLNHPFVGAMADAWGQQVVQ
                     DSHDSPNERLTSMMLSAWGRLPTDSELRTWDTAVRDFASNPDQIMSDAAAWSTVARAL
                     FNSKEFLYYR"
     assembly_gap    1245..1315
                     /estimated_length=71
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1316..1833)
                     /locus_tag="PK264_20630"
     CDS             complement(<1316..1833)
                     /locus_tag="PK264_20630"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HRD78309.1"
                     /translation="MLLNEASSDPAITALVSEYRRIESTINFPRSANSFDERGVEPVD
                     YRLNIRGDVYDEGPAVPRGYLEVFGGPAIVQGSNGSGRLELAEALASPTNPQTARVYV
                     NRVWSWIFGKGIVSTPNDFGKLGDQPSHPELLDWLAIQFMEEGWSTKDLIRRLVLSRT
                     FRQTGDVTAQGL"
     gene            2011..2655
                     /locus_tag="PK264_20635"
     CDS             2011..2655
                     /locus_tag="PK264_20635"
                     /inference="COORDINATES: protein motif:HMM:NF019180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HRD78310.1"
                     /translation="MFRVLLDGRPVRTPAKALVVLPTERLARAVAAEWEAQGDEIKPA
                     SMPLVRLVNSALDGVRGREAEVAADIAKYAASDLVCYRAESPAELVQRQVAHWDPLLD
                     WAAEALGCRLARTTGLMPVTQAEAAIKAVLAAIDAKDIYRLAALHVATTLLGSAILGL
                     ALMRRRISGDAAWTAAHVDEDWQAEQWGHDGEAEERRAARRRDLGAAVTIIELA"
     gene            2768..3298
                     /locus_tag="PK264_20640"
     CDS             2768..3298
                     /locus_tag="PK264_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78311.1"
                     /translation="MADQLHDLASCACAPVDEEHGRMAGSLCGQDLRRRTIGRLVGSE
                     DEPQCRLEPETMGQVDIADAGGTTEVGFEVGLEALRTTLEAASRDVGVMTQPQNGVAL
                     ARDGHVKVARARAEDADGGIAETHGPALALHTIEPGLLGEGEPCGGDARDVEAMAWGD
                     GVDRRCRQRDQHDKIS"
     gene            complement(3512..3919)
                     /locus_tag="PK264_20645"
     CDS             complement(3512..3919)
                     /locus_tag="PK264_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78312.1"
                     /translation="MVEVFKVNDELQWGRLVKSWATGVNHLTGGFALPAVPRTLDDLK
                     AACHAVGIACTVPSHHKALAVLSSSEEVLVIRLPAKSVTDETHKELGNGAAYSLPSFY
                     DDFYGKPLPAMTPAKSQEFHAARIGDYSVRMCA"
     gene            complement(3996..5750)
                     /locus_tag="PK264_20650"
     CDS             complement(3996..5750)
                     /locus_tag="PK264_20650"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HRD78313.1"
                     /translation="MHGLNLALSHVGPRSTLESGEQIASGSSTIEGLRHFLKPRPDQR
                     FSADGRVESYIAGNGDPLRLLLRKLSSTLLFEDFWHDKPLPAGIAGPLPFDNPQVPSA
                     YTYLLQLAAHDLVHSSVSLAGTGADLGVSGIVNSRNVPLTLETIYGGGPEVCPHAYKI
                     DQAFVSSGGRVSRTRLRLGRLRIDNRLDASCPMRDVARGEPLHASDDPHRDPSASVWP
                     SEALVADPRNDDHAILSQLTVLFHLLHNHLVDRMEALGLPSDGPSEQQAFRRFLAART
                     AVTLIYRHILRNDVLPRILHPDVTSWYGSNSPLEVAHGIPLEFTHGAFRFAHAMVRDR
                     YSVTRDAPLDTERALQQSSHRGFGFLPVSTEWRVDWRRFVPVPGHPMPMNLSRRIGPS
                     YPGVLSSSRLFPVLAPEAGAASLPYGLIGRDLMSASLAGLWSVPALTAELQGRLGAGL
                     VPDFAAWQAALGDWLTAQSVFDAAEVATLAADPPLPFYCLFEASKTLRAGLPHDTLGG
                     LKLGVIGSIIVADTFHGALQRNPVAFDAAATGALLKPRLLAMSAAILGAPAPFATLPE
                     IGDFGTLVAYLIDSATLP"
     gene            complement(5807..>6316)
                     /locus_tag="PK264_20655"
     CDS             complement(5807..>6316)
                     /locus_tag="PK264_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78314.1"
                     /translation="RLRAVGKRYAAAARRARIGEGGKCFSEEGLSIIGIGGKASVCHP
                     ECGTTADKPFTGPVEMEQPTARLDDEDRNRKLVQHRREQHLESRARTDRALAEHHALR
                     EFRKAAETCRCACLILGHSTLLSCRSICGTSTTNIAAEAALARGSQNERERTVSGAAP
                     RWRCRFTLD"
BASE COUNT         1120 a         1916 c         2168 g         1041 t
ORIGIN      
        1 ccgaagcagc gaacgcgcga tcctgttgac cgattaactc caagaggcgc cgttgttgcg
       61 gcgtctccag cgccgggacg atgttatcca gcggaacccc gttggcccga atcatgctgg
      121 cctggtgcga cgcggggaga taagaactgc cgaagttttc gagtccgccg ttcggaaccc
      181 agtcgttatt caaaaccaca tagccgggga gattctcatt ctcggttccc aggccgtagt
      241 tgacccaggc tcccatgctg ggggcctgcc cgaggattcg gccggtgtgc agcagcaggt
      301 tctgttgccc atgcaacggt agttcgccgg tcatggagcg gacaacacag agttcgtcag
      361 ccacctgggc gatgcgcgga aagagatcgc tcacccagag tccgctttcg ccgcgttgcc
      421 ggaactccca cgggcacccc agccagacac gattggcgct cgactgcgaa agattcgaga
      481 cagccgattc gccgatcggc ttcccttcgt gttccttaag catgggcttg ggatcgaacg
      541 tatcaacatg gctggggccg ccatccatga agcagaagat gatgcttctg gcgcgcgctc
      601 gatgatggcg cgttccgcct tgtgccaagc cggctagcgc gagccagccg aatccggccg
      661 acagcgactg aagaacggtt cgacggctga accggtgatc gacgagagga tgaaatgtcg
      721 acatgctgtt caccggtagt agagaaattc tttgctgttg aacaaggcgc gagccacggt
      781 tgaccaggca gcagcatcgg acatgatctg gtcgggattc gaggcgaagt ctcggaccgc
      841 ggtgtcccac gttcgcaatt cgctatcggt cggtaaccgt ccccaggccg agagcatcat
      901 gctcgtcagg cgttcgtttg gcgaatcatg gctgtcttgc acaacttgtt gtccccaggc
      961 atcagccatc gcgccgacga agggatgatt taacaggatg agggcttgag ccgggacatt
     1021 ggtgacatcg cgcgccccgt tcggctgctt cagatccggc agattgaagc cgacgaggaa
     1081 ttcgggcggc tgcatgacgg acatttcgag atagagcgat cggcgacgat ccccatcgag
     1141 cggacccgag aagagtcgct tctggggatc ttcagcagga cgatgcgggc ggatcgggcg
     1201 gccatggagt gagggatcaa gttggcccga aactgcgagc aggcnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgcaa
     1321 accctgagcg gtaacgtcac ccgtctgccg gaatgtgcgg ctgaggacca ggcgacggat
     1381 gaggtcctta gtcgaccatc cttcctccat gaactgaatc gcgagccagt cgagcaattc
     1441 cggatgcgag ggctgatcgc ccagtttgcc gaaatcattc ggtgtgctga cgattccctt
     1501 accaaaaatc cacgaccaga cccggttgac gtagacacgg gctgtctgag gattggtcgg
     1561 gctggcgagt gcctcggcca gttcgagccg tccgctgccg tttgaaccct ggacaatggc
     1621 cggcccgcca aaaacttcca ggtagccgcg cggcacggcc gggccttcat cgtaaacgtc
     1681 gccccgaatg ttcaatcggt aatcgaccgg ctcgaccccg cgctcatcga agctattggc
     1741 gctgcgggga aagttgatcg tggactcaat cctgcgatac tcgctgacca gcgcggtgat
     1801 ggccggatcg gacgaggcct cgttcagcag catgccgtgt tcgagcatcc aggtcagaag
     1861 tttcgcatca cccttgcgtg gctcatttcg acaccagcga tccacggcac cacgtaacca
     1921 tgtcgtgagg tatcgttgca actctccagc attcgccgga gccggttgtg agagcagggg
     1981 ttcgagccat tcgagttcga tgcacgggcg atgtttcgcg tgcttctgga cggccggccg
     2041 gtccgcacac cggcgaaggc cctggtggtc ctgccgacgg aacgtctcgc acgagccgtc
     2101 gccgccgagt gggaggcaca gggcgacgag atcaagccgg cgagcatgcc gctcgtccgc
     2161 ctcgtcaaca gtgccctcga tggcgtgcgc ggccgcgagg ccgaggtcgc cgccgacatc
     2221 gcgaagtatg cggcgagcga cctcgtctgc tatcgggcag aaagtccggc cgaactcgtc
     2281 cagcgccagg tcgcccactg ggatccgctc ctcgattggg cggccgaggc gctcggctgc
     2341 cgtctcgcgc ggacgacggg tctgatgccg gtcacgcagg ccgaggccgc gatcaaggcg
     2401 gtcctggcgg cgatcgacgc caaggacatc taccggctcg cggcgttgca tgtcgcaacg
     2461 acgctgctcg gatctgcgat cctgggcctc gccctcatgc ggcgtcggat ttccggtgat
     2521 gccgcgtgga cggcggctca tgtggacgag gactggcagg ccgagcagtg gggccacgac
     2581 ggcgaggccg aagagcgtcg tgcggcccgc cgccgcgatc tcggtgcggc ggtgacgatc
     2641 atcgagctcg cgtgattgtc gcgctccatc cggcgatccg aggcgctggc gcggctactg
     2701 gtggagcccc atcgaagact atgcgcgtac tcctcgttgg ccaacctgcc agcacgtgcg
     2761 ccaccctttg gccgaccagc tgcatgacct cgccagttgt gcgtgcgcgc ccgttgacga
     2821 agaacatggc cggatggcgg gcagcctctg tggccaagac ctccggcgca ggacgatcgg
     2881 gcgtcttgtc ggcagcgagg atgagcctca atgtcggctc gagcccgaga ctatgggcca
     2941 ggtagatatc gccgacgccg gcggcacgac cgaggtgggc ttcgaggtcg gacttgaggc
     3001 tttgcgcacg acgctcgagg cagcgtctcg cgatgtcggc gtcatgacgc agccccagaa
     3061 tggcgtcgcg ctcgctcgtg acgggcacgt aaaagtggcc cgcgctcgtg cggaagatgc
     3121 ggacggaggc atcgccgaga cccacggccc cgccctcgcg cttcacacaa tcgagccagg
     3181 tctgctcggt gaaggcgagc cgtgtggcgg ggatgcgcgt gacgtcgagg cgatggcttg
     3241 gggcgacggc gtcgacagga gatgtcgcca gcgcgatcag cacgacaaga tctcgtgata
     3301 cttcgaccag cggactcacg gccgtcactc cggcaagcaa caccactgcg accgacggaa
     3361 cctcatcccg tcacggcaat gttacgagca cggagtgaac gagccgtaaa cgcgcgtccc
     3421 ggagcggggc gctgggtcgg gaatcatctc cgctacaagg ccaagttcgt gcgctggtat
     3481 tgcgctctcc acgtctccaa cgagatcgag gtcaggcaca catccgcact gagtagtcgc
     3541 cgatgcgggc cgcgtggaac tcctgcgact ttgccggtgt catggccggc agcggcttac
     3601 cgtagaagtc gtcgtagaag cttggcaggg agtaggcggc gccgttgccg agctccttgt
     3661 gcgtttcgtc cgtcaccgac ttcgcgggca ggcggatcac cagcacctcc tcgctcgagg
     3721 agagcacggc gagtgccttg tggtggctcg gcaccgtgca ggcgatgccg accgcgtggc
     3781 acgcggcctt gagatcgtcg agcgtgcgcg gcacggccgg cagcgcgaag ccgcccgtca
     3841 gatggttcac gcctgtggcc cacgatttga cgagccggcc ccactgcaat tcatcgttca
     3901 ccttgaagac ttcgaccatc gggcaaacct ccgaacatat gggtgctttt ctggcgtcgg
     3961 cggcagcccg gcacccgtcg ggccgccacc ggagctcagg gcagcgtggc gctgtcgatc
     4021 aggtatgcga cgagggtgcc gaagtcgccg atctcgggaa gcgtcgcgaa cggggccgga
     4081 gcgccgagga tggcggcgga catggcgagg aggcgcggct tgagcagggc gcccgtcgcc
     4141 gccgcgtcga aggcgacggg attgcgctgc agggcaccgt ggaacgtgtc ggcgactatg
     4201 atcgagccga tgacaccgag cttcagtccg cccaatgtgt catgggggag gccggctcgg
     4261 agcgtctttg aggcttcgaa caggcaatag aacgggagcg gcggatcggc ggcgagtgtc
     4321 gccacctcgg cggcgtcgaa aaccgactgc gccgtgagcc agtccccgag cgccgcctgc
     4381 catgcggcga aatctggtac gaggccggcg ccgaggcggc cttggagctc ggcagtgagg
     4441 gcggggacgc tccagaggcc ggcgagcgat gcgctcatga gatcgcggcc gatgaggcca
     4501 taggggaggc tcgcggcacc tgcctccggg gcgagcacgg gaaagagccg cgaggaggag
     4561 aggacgcctg ggtagctcgg gccgattcga cggctgaggt tcatcggcat gggatggccc
     4621 ggcacgggca cgaagcggcg ccagtcgacc cgccactccg tcgagaccgg caggaagccg
     4681 aagccgcgat gcgaactctg ctggagggca cgttccgtgt cgaggggtgc gtctcgcgtg
     4741 acggaatagc ggtcgcggac catggcatgc gcgaagcgga acgcgccgtg cgtgaactcg
     4801 agcggaatgc cgtgcgcgac ctcgaggggc gagttgctgc cgtaccagga agtgacatcc
     4861 ggatggagga tgcgcggcag cacgtcattg cgcaggatgt ggcgatagat cagcgtcacc
     4921 gccgtgcgcg ccgcaaggaa acggcggaag gcctgctgct cggagggacc gtcggagggc
     4981 aagccgagcg cctccatgcg atcgacaaga tgattgtgga ggaggtggaa gaggacggtc
     5041 agctgcgaga gaatggcgtg gtcgtcattg cgcgggtcgg cgacgagggc ctcgctcggc
     5101 cacacgctag ctgaagggtc gcgatgcgga tcgtcgctcg catgcagcgg ctcgccgcgt
     5161 gcgacgtcgc gcatcggaca gctcgcatcg aggcggttgt cgatgcgcag ccgaccgagc
     5221 cggagccggg tgcgcgagac gcgaccaccc gagctaacga acgcctgatc gatcttataa
     5281 gcgtgagggc agacctcggg gccgccgccg tagatcgtct cgagcgtcag cggcacgttt
     5341 cggctgttga cgatgccgct gacgccgagg tcggcgccgg taccggcgag cgagacggag
     5401 ctgtggacga gatcgtgggc ggcgagctgg aggagatagg tgtaggcgga cggaacctgc
     5461 gggttatcga acgggagcgg cccggcaatt ccggcgggca gcggcttgtc gtgccagaag
     5521 tcctcgaaaa ggagcgtgct cgagagcttg cgcagcagga gccgcaaagg gtcgccgttg
     5581 ccggcaatgt agctctcgac acgtccgtcc gcggagaagc gctgatcggg gcgcggcttc
     5641 aggaagtggc ggaggccctc gatggtcgac gagccgctcg caatctgctc gccactctcg
     5701 agcgtgctgc gcggtccgac atgcgacagc gccagattga gaccgtgcat gtgctcctcc
     5761 ccgtccccaa acgccaccca ctttggagat tgaacccgtc agagcgtcaa tcgagcgtga
     5821 accgacaacg ccaccgcggt gcggcgccgc tcacggtgcg ttcacgctcg ttctgcgagc
     5881 ctctcgccaa ggcggcctcc gccgcgatgt tggtggtcga cgtcccgcat atcgagcggc
     5941 atgacaggag ggtcgaatgc cccaggatca ggcatgcgca acggcaggtt tcggcagctt
     6001 tgcggaattc gcgcagggca tgatgctcgg cgagcgcccg atccgttcgg gctcgggact
     6061 cgagatgctg ctcgcggcgg tgctggacga gcttgcggtt gcgatcttca tcgtcgagcc
     6121 gagcggtcgg ctgctccatc tcaaccgggc cggtgaaagg cttatccgcc gtggtaccgc
     6181 actcagggtg gcaaaccgag gccttgccgc ctataccgat gatgctcagt ccctcctcgg
     6241 agaagcactt tccgccctcg ccgatcctcg cgcgccgcgc tgccgcggcg tagcgcttgc
     6301 cgacggcacg gaggcg
//
LOCUS       DAORNH010000275         6314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9148_length_6314_cov_2.676466, whole genome shotgun sequence.
ACCESSION   DAORNH010000275 DAORNH010000000
VERSION     DAORNH010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9148_length_6314_cov_2.676466"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK264_20660"
     CDS             complement(<1..220)
                     /locus_tag="PK264_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78315.1"
                     /translation="MGSSDRLLASNRRASTRRAVRGSAALAVAAMMAASLPSMMASAQ
                     SHGIAGALYVVKQQGNALQVRMELQADCL"
     gene            639..3119
                     /locus_tag="PK264_20665"
     CDS             639..3119
                     /locus_tag="PK264_20665"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HRD78316.1"
                     /translation="MSTKGEVAKALGRVELGIRKGSRIAPLMLLLLMSLLFIIYGLPS
                     GFDQLGASLKLTQSPNDPLTLDKLAKALEKPDAFQKIMATISSMSAWEFVTKIVRPAI
                     KVYIDQLISQSGMIITSVVKSVLQLGLYALLPGLAGLVYRRNFLGWFLTSFAVLAGIN
                     ASGAFGSLTTAQPMPGAGAILVFVLSQLLILILAYRLRRHSQGLNVLPPRIQNWGLTA
                     VLVVVGIACWQGWGPGYSSGGSKRASAEPEFRILQVTPVPTTADDAKRAPDPGKTTPA
                     PPSSTPAPLPAGPGAAAPKKADTDAKPAPTSSAPTPPTTTAPRKADSGAAVPKTAAGT
                     SSSDAGLSASTGATRSWIWSFLGTGVSGWIYKWEFILLGLPIIYTLLRNSAFWTGRTP
                     KNIVICLDGTSNTPDQIEMGFLAQTNVFKLFNMLKADKAGAYQPIGSFDASLAKRFGD
                     KQMAFYYAGVGNKYDNDPIFQTLGMATGMGAADIVERAYIDLVRVYKPGDRVFIFGFS
                     RGAAISRLLARTIDARGAPRSMWTLRLFGKHRCLWRSTRRDPVPIDVLGCWDTVGSFG
                     VAKTIAGINFQQLNMFRDLSIPDNVAQAYHMVALDEMRDSFEPTLMDPDPIRPERIVE
                     VWFSGDHANIGGGWATDKLSDITLDFLLRRISSGYATDAAATPGDENWGLYLSAIKAD
                     KMAATAVAEPGAVATVDPDPLGQVRSWFSNLYVYRPRKLPLYAVISESVFDRMTKAMP
                     VYAPQSLFDLNDALDAKRDLIEAKVAKLTETNSLDDEERKAIIEFNRKLRLSRFPKYW
                     EALVAERAPQPVAVVLSNAELNVTKAAA"
     gene            complement(3132..>4041)
                     /locus_tag="PK264_20670"
     CDS             complement(3132..>4041)
                     /locus_tag="PK264_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRD78317.1"
                     /translation="IQRPGGDAAIVRLHGSRKGVAMCCDVTPRYVLADPEMGTRQAVA
                     ETWRNLTAVGADPIAITDNMNFANPQRPEVMGQFVGACKGMADACRTLDYPVVSGNCS
                     LYNETNGVGIPPTPAIGGVGLVPDVTKTAGLALERSGDLLILIGREQGWLGQSLYQQH
                     ATGKLEGAPPPVDLAEELKAGGLVRNLIRDGKVSAVHDVSDGGLIVAVAEMALAGRWG
                     AELFPYEGKLPAHAVWFGEDQGRYVLSAAPGVAEEIVERARLLALPARIIGKVAGTML
                     AMKGETALPLAKLRATHEAWLPKLMN"
     assembly_gap    4042..4205
                     /estimated_length=164
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4206..>4439)
                     /locus_tag="PK264_20675"
     CDS             complement(<4206..>4439)
                     /locus_tag="PK264_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRD78318.1"
                     /translation="GMTAYEMMLSESQERMLMILRPGSEAAAEAIFRKWGLDFAIIGR
                     TTDTGRMIVKHKGEVEADLPVDKLANSAPLYERP"
     assembly_gap    4440..4560
                     /estimated_length=121
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4561..>4819)
                     /locus_tag="PK264_20680"
     CDS             complement(<4561..>4819)
                     /locus_tag="PK264_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRD78319.1"
                     /translation="AAYNNNILVNAMCVGIAKADKIFYSAAKGVGLPVVYVGSKTGRD
                     GIHGATMSSKEFDDKTEEKRPTVQVGDPFTEKLLIEACLELM"
     assembly_gap    4820..4885
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4886..5347)
                     /locus_tag="PK264_20685"
     CDS             complement(<4886..5347)
                     /locus_tag="PK264_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRD78320.1"
                     /translation="MSGSAPITSKITPDLVAEHGLSPDEYQRLLGVLGREPTFLELGI
                     FSVMWSEHCSYKSTRVWLKTLPTTGKRVIQGPGENAGVVDLGDGDVCVFKMESHNHPS
                     YIEPFQGAATGVGGIMRDVFTMGARPVANLNALRFGAPDHPKTRHLVSGVVA"
     gene            complement(5540..>6314)
                     /locus_tag="PK264_20690"
     CDS             complement(5540..>6314)
                     /locus_tag="PK264_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78321.1"
                     /translation="AVRKGDHYIVSGQKIWTTLAQHADWIFCLVRTDPAAKKQLGISY
                     IVFEMKQKGVTVRPIQTIDGSREVNEVFFDDVKVPVENLIGEENRGWDCAKFLLGNER
                     TGIARVGASKLRVRRIKELARKVSVGEGTLWDDMQFRERVAAVEVELKALEMTQMRVL
                     AAEKGRRDGKPNPASSILKIKGTEIQQATTELLMHIAGPFAVPFASDEEAFGHNEQPF
                     GPEWAAATAPTYFNYRKTSIYGGSNEIQRNIIAKAVLGF"
BASE COUNT         1004 a         1996 c         1884 g         1079 t
ORIGIN      
        1 cgaggcaatc cgcctgcagc tccatgcgca cctgcagcgc gttgccctgc tgcttgacca
       61 catacagcgc gcccgcgatc ccgtggctct gcgccgaggc catcatcgag ggcagggatg
      121 ccgccatcat ggccgcgacc gcgagggcag cagagcctcg caccgcgcgg cgggtcgatg
      181 cgcggcggtt cgatgccagc agccgatcgc ttgagcccat agccttgcct ccgcaacatc
      241 gtaagcgcct gcggccggag tgcagtgcgc accgctcgcc gaagccaacc accttcgcgt
      301 ctgatcgccg ttgttggcga ttcgtcgttc ccggcacagc gcaatgcgtc gccgcctgtg
      361 caacttatca acagtcagag tggcgcgcac gtggcagagc gcgctgtcta tgacgagagg
      421 cgttgtgacc agcacctcga cacgccctct taggctcact gtggcgattt tagcacagca
      481 actgctgtat cggtgtttcc gatcatgtga gcaatgtctc ggcgatggcc agtactcagc
      541 tattgtcgca acatggaaga tgaagtggaa caacaataag tccgtcaaca gagctaaatt
      601 tgcacctcca ggtgtagtgg gtgggagggg tctgcgtcat gtcgacgaag ggtgaagtgg
      661 ccaaggcgct tgggcgtgtc gaactcggca ttcgcaaggg atcgcgaatt gctccgctga
      721 tgcttctgct gctgatgtct ctgctgttca tcatctacgg attgccgtcc ggtttcgatc
      781 agctcggcgc cagcctcaaa ctgacccaat cgccgaacga cccgctcacc ctcgacaagc
      841 tcgcgaaggc gctcgagaag ccagatgcgt tccagaagat catggcaacg atctcgagca
      901 tgagcgcctg ggagttcgtc accaagatcg tgaggcccgc gatcaaggtc tacatcgatc
      961 agctgatctc tcaatccgga atgatcatca cgagcgtcgt gaagtcggtc cttcaactcg
     1021 gcctctatgc gcttctgccg gggctcgcag gtctcgtcta tcggcgaaat ttcctcggct
     1081 ggttcctcac gtccttcgct gtcctcgccg gcatcaatgc atccggcgcg tttggctcgc
     1141 tgaccacggc gcaaccgatg cccggcgcgg gtgcgatcct cgttttcgtg ctctcgcagc
     1201 ttctgatcct gatcctcgct taccgtctcc gccgtcactc gcaggggctc aacgtcttgc
     1261 cgccccgcat ccagaactgg ggcctgaccg ccgtcctcgt cgtcgtcggc atcgcctgct
     1321 ggcagggctg gggacccggc tacagctcgg gtgggtcgaa gcgcgcgtcg gctgagcccg
     1381 agttccgcat ccttcaggtc acgcctgtac cgacaactgc cgatgatgcg aagcgtgctc
     1441 ccgatccggg caagaccacg cctgcaccgc catcgagcac gcccgcgccg ttgccggcag
     1501 gaccgggcgc tgcagcgccg aagaaggcgg acacagatgc gaagccggcg ccaacgtcat
     1561 ccgcgccgac accgcccacg acaacagcgc cgcgcaaggc tgatagcggc gccgcagtac
     1621 ccaagacggc tgcgggaaca tcgagcagtg atgccggcct tagcgcgtcg accggtgcga
     1681 cacggagctg gatctggtcc ttcctcggca caggcgtctc cggctggatc tacaagtggg
     1741 aattcatcct gctcggcctg ccgatcatct acacgctgct gcgcaactcc gccttctgga
     1801 cggggcgcac cccgaagaac atcgtcatct gtctcgacgg cacgtccaac acgccggacc
     1861 agatcgagat gggcttcctc gcccagacca acgtcttcaa gcttttcaat atgcttaagg
     1921 ccgacaaggc cggtgcctac cagccgatcg gcagcttcga tgcctcgctc gccaagcgct
     1981 tcggcgacaa gcagatggcc ttctactacg cgggcgtcgg caacaagtac gacaacgacc
     2041 cgatcttcca gacgctcggc atggcaaccg gcatgggtgc cgccgacatc gtcgagcgcg
     2101 cctacatcga tctcgtgcgc gtctacaagc cgggcgatcg cgtcttcatc ttcggcttca
     2161 gccggggcgc cgccatctcg cgactactcg cgcgcaccat cgatgcgcgc ggggcgccgc
     2221 gctccatgtg gacgctgcgc ctcttcggaa agcaccgctg cctctggcgc tcgacgaggc
     2281 gcgatccggt tccgatcgac gtgctcggct gctgggatac cgtcggatcg ttcggagtcg
     2341 ccaagacgat cgcgggcatc aatttccagc agctcaacat gttccgcgat ctctcgatcc
     2401 ccgacaacgt ggcgcaggcc tatcacatgg tcgcgctcga cgagatgcgc gacagcttcg
     2461 agccgacgct catggacccc gatccgatcc gtcccgagcg gatcgtcgaa gtgtggtttt
     2521 ccggcgatca tgccaacatc ggtggcggct gggcgaccga caagctctcc gacatcacgc
     2581 tcgacttcct gctccgccgc atctccagcg gctatgccac cgacgcggcg gcaacgccgg
     2641 gcgacgagaa ttggggccta tatctcagcg ccatcaaggc cgacaagatg gccgcgaccg
     2701 ccgtcgccga gccaggtgcc gtcgcgacgg tcgatcccga tccgctcggc caggtgagga
     2761 gctggttctc gaacctctac gtctacaggc cacgcaagct gccgctttac gccgtcatct
     2821 ccgagagcgt gttcgaccgc atgaccaagg ccatgcccgt ctacgcgccg cagagcctct
     2881 ttgacctcaa cgacgcgctc gatgcgaagc gcgacctgat cgaggcgaag gtcgccaaac
     2941 tcaccgagac caactcgctc gacgacgagg agcgcaaggc gatcatcgag ttcaaccgga
     3001 agctccgcct ctcgcgcttc ccgaagtact gggaggcgct ggttgccgag cgtgcgccgc
     3061 agccggtcgc ggtcgtcctt tcgaacgctg agctcaacgt gaccaaggcg gcggcatgag
     3121 acggcagcgc ctcaattcat gagtttcggc agccaggcct catgcgtcgc gcggagcttg
     3181 gcgagcggca acgccgtctc gcccttcatg gcgagcatgg tgccggcgac cttgccgatg
     3241 atgcgcgcgg ggagggcgag gaggcgcgcc cgctcgacaa tctcctcggc gacgccgggc
     3301 gccgccgaga gcacgtagcg gccctggtcc tcgccgaacc agacggcgtg ggccgggagc
     3361 ttgccctcgt aggggaagag ttcggcgccc caccggcccg cgagcgccat ctcggcgaca
     3421 gcgacaatga ggccgccgtc cgagacgtcg tgcacggccg ataccttgcc gtcgcggatg
     3481 aggttgcgca caagcccgcc cgccttcagc tcttcagcga ggtcgaccgg aggcggcgcg
     3541 ccctcgagct tgcccgtcgc gtgctgctgg tagagcgatt ggccgagcca gccctgctcg
     3601 cgcccgatca ggatcaggag gtctccgctc cgctcgaggg cgaggcccgc ggtcttcgtg
     3661 acgtcgggaa cgaggccgac gccgccgatc gcgggtgtcg gcgggatgcc gacgccattc
     3721 gtctcgttgt agagcgagca gttgcccgac acgaccggat agtcgagcgt gcggcaggcg
     3781 tcggccatgc ctttgcaggc gccgacgaac tggcccatca cttccgggcg ctgcggattg
     3841 gcgaagttca tgttgtcagt gatggcgatc ggatcggcac cgacggctgt cagattgcgc
     3901 caggtctcgg cgacagcctg ccgcgtcccc atctcgggat cggccagcac gtagcggggc
     3961 gtcacgtcgc agcacatggc gacgcccttc cgcgagccgt ggagccgcac gattgccgca
     4021 tcgccgccgg ggcgctgaat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnggccg ctcgtagagg ggtgccgagt tcgcgagctt gtcgacgggg aggtcggcct
     4261 cgacctcgcc cttgtgcttg acgatcatgc ggcctgtgtc ggtcgtgcgg ccgatgatgg
     4321 cgaagtcgag cccccacttc ctgaaaatcg cctcggcggc ggcctcggag cctggcctga
     4381 ggatcatcag catgcgctcc tggctttcgg agagcatcat ctcgtaggcc gtcatgccgn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 catcagctcg aggcaggcct cgatcaggag cttctcggtg aaggggtcgc cgacctgcac
     4621 ggtcgggcgc ttctcctcgg tcttgtcatc gaactccttc gacgacatgg tggcgccatg
     4681 gataccgtcg cgcccggtct tcgagccgac gtagacgacc ggcagcccga cgcccttcgc
     4741 ggccgagtag aagatcttgt cggccttggc gatgccgacg cacatggcgt tgaccagaat
     4801 gttgttgttg taggccgcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnncgcga caacgcccga gaccaagtgg cgcgtcttcg
     4921 gatgatcggg cgcgccgaag cggagcgcat tgaggtttgc aacaggcctc gcccccatcg
     4981 tgaagacgtc gcgcatgatg ccgccgacgc cggtcgccgc gccctggaac ggctcgatgt
     5041 agctcggatg gttgtggctc tccatcttga agacgcagac gtcgccgtcg ccgaggtcga
     5101 ccacacccgc gttctcgccc ggcccctgga tgacgcgctt gcctgtcgtc ggcagcgtct
     5161 tcagccagac gcgcgtcgac ttatatgagc agtgctcgga ccacatcact gagaagatgc
     5221 cgagttcgag gaaggtcggc tcgcgcccga gcacgccgag cagccgctgg tactcgtccg
     5281 gcgagagccc atgctcggcg acgagatccg gcgtgatttt ggaggtgatg ggggcgcttc
     5341 cagacatggg ctcgctcaag tggacaagag ggcggtctcg cgcgccttat agcgagtcct
     5401 ggccggatgg gcagcccttt ggttgcaccc cttggtagca cggggacagg acccatctcg
     5461 accccgcaca tgctcagcga ttcgcgggtt cgcgcgacga ccacgcgcct ccgcgcacgt
     5521 tccactcgcc gcgacgatct cagaacccga gcacggcctt ggcgatgatg ttgcgctgga
     5581 tctcgttcga gccgccgtag atcgaggttt tccgataatt gaagtaggtc ggggcggtgg
     5641 cggcagccca ctctggcccg aacggctgct cgttgtgccc gaacgcctcc tcatccgatg
     5701 caaatggcac tgcaaacggg ccggcgatgt gcatgaggag ctcggtcgtc gcctgctgga
     5761 tctcggtgcc cttgatcttc aggatcgagg aggcgggatt gggtttgccg tcgcgccggc
     5821 ccttctcggc ggcgagcacg cgcatctgcg tcatctcgag tgccttcagc tccacctcga
     5881 cggcggcgac gcgctcgcgg aactgcatgt cgtcccagag cgtgccctcg ccgaccgaca
     5941 ccttgcgtgc gagctccttg atgcgccgga cgcgcagctt cgaggcgccg acccgggcaa
     6001 tgcccgtgcg ctcgttgccg agcaggaatt tcgcgcagtc ccagccgcgg ttctcctcgc
     6061 cgatcagatt ctcgacgggc accttcacgt cgtcgaaaaa gacctcattt acctcgcgtg
     6121 agccgtcgat cgtctggatc gggcgcacgg tcacgccctt ctgcttcatt tcgaagacga
     6181 tgtaggagat gccgagctgc ttcttggcgg ctggatcggt gcgcaccagg cagaagatcc
     6241 agtcggcatg ctgggcgagc gtcgtccaga tcttttgccc gctgacgatg tagtgatcac
     6301 ccttgcgcac agcc
//
LOCUS       DAORNH010000276         6278 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9229_length_6278_cov_2.827897, whole genome shotgun sequence.
ACCESSION   DAORNH010000276 DAORNH010000000
VERSION     DAORNH010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9229_length_6278_cov_2.827897"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="rpsK"
                     /locus_tag="PK264_20695"
     CDS             <1..312
                     /gene="rpsK"
                     /locus_tag="PK264_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRD78322.1"
                     /translation="FNNTMITITDAQGNTIAWSSSGTKGFKGSRKSTPFAAQMAAEDA
                     GRKAQEHGMKVLEVEVCGPGSGRESALRALQSIGFQITSIRDVTPIPHNGCRPRKRRR
                     V"
     gene            444..1448
                     /locus_tag="PK264_20700"
     CDS             444..1448
                     /locus_tag="PK264_20700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRD78323.1"
                     /translation="MLQKNWQGLIKPTKLQVMPGRDRDRVATIVAEPLERGFGMTLGN
                     AIRRVLLSSLQGSAVSAVHIDGVLHEFSSIPGVREDVTNIILNIKDIALRIHSDGVKR
                     MVLKKDSRGVAKAGDIEAGSDVEILNPEHVICTLDQGAAIRMEFTASTGKGYVSAERN
                     APIGLIPVDSLFSPVKKVSYRIENTREGQILDYDKLTMTVETDGSVKAEDAVALAARI
                     LQDQLAVFINFEEPKKQVEEAKHPDIPFNAALLKKVDELELSVRSANCLKNDNIVYIG
                     DLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPNWPPDNIEELAKRYEDQY
                     "
     gene            1577..2020
                     /gene="rplQ"
                     /locus_tag="PK264_20705"
     CDS             1577..2020
                     /gene="rplQ"
                     /locus_tag="PK264_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRD78324.1"
                     /translation="MMHGKRGRRFSRDSGHRQAMFSNMAAALIKHEQIVTTLAKAKDL
                     RRVMDKYITLAKRGDLNSRRLAASRLRDEDMVKKLFGVLGPRYKERSGGYTRVLKAGF
                     RYGDSAPRAVIELVDRDVTVKGAEDRARHEADVKAREAATAPAAV"
     gene            2130..3050
                     /locus_tag="PK264_20710"
     CDS             2130..3050
                     /locus_tag="PK264_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HRD78325.1"
                     /translation="MRLRFHTLDVFTDQRFGGNPLAVVHDADALDTKAMQAVAREFNL
                     SETVFVLKPERPAHSAKVRIFTPAAELPFAGHPTVGTAAILAELRAAQTGGGANGDSL
                     VVLEQQIGTVRVGVRMRAGSAPFAEFDAPRLPEETGQLAPIDRLAAAIGLIPAEIGFE
                     NHRPTRFAAGNAFAFIPVAGLDAIARAAVQAQHWQSAFGGQGLIGAFLYTRQCVQGTS
                     AFHARMFGPDVGVIEDPATGSAAVGFAGAVYRFDGIPDGMHKRIVEQGFEMGRPSLIT
                     LSLTIERGRLDTVRIGGQSVRVSEGTIDVS"
     gene            complement(3064..3624)
                     /locus_tag="PK264_20715"
     CDS             complement(3064..3624)
                     /locus_tag="PK264_20715"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRD78326.1"
                     /translation="MTTPPGARRIDISIVVAMARNRVIGRNGQLPWCVPSDLATFRRL
                     TLGKPVIMGRKTFQSLAKPLPGRMNLVVTRDPTLRTGIGAIEVAPDTTVAAFASFDAA
                     IVSALTRAATNGIDEVMIIGGAEVYRAAFPRASRIYLTEIDAAPDGDTHFPELDDAIW
                     REVSRVLIPPHPRDEYAATLVTYDRR"
     gene            complement(3621..4127)
                     /locus_tag="PK264_20720"
     CDS             complement(3621..4127)
                     /locus_tag="PK264_20720"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRD78327.1"
                     /translation="MGQVTITVNGRTYRLTCGDGEEARLIQLADDLRARVDGIIAEFG
                     HAGDDRLMLMAAIMTLDELFDLRARLASLQGPPLPVETIASNLVPPYAPQPPAANSRP
                     GDTYSGAYVDQPPPPPPPPSRGFDANPSSAFHEHPPGPYVTAPASNTTVISRPAAPVR
                     TPQPPRRR"
     gene            complement(4183..4437)
                     /locus_tag="PK264_20725"
     CDS             complement(4183..4437)
                     /locus_tag="PK264_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78328.1"
                     /translation="MAERGAKKSRPRAPASAAREAPRDTVVSRESLVERVRDLELERE
                     GLKSELAAANARLRALEGRINEAVARVDSALDSLHKIARG"
     gene            4746..5756
                     /gene="gap"
                     /locus_tag="PK264_20730"
     CDS             4746..5756
                     /gene="gap"
                     /locus_tag="PK264_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRD78329.1"
                     /translation="MAVKIAISGFGRIGRNVLRAIAESKRNDVEVVAINDLTSPDVNA
                     HLFRHDSVHGNYAGTVKLDGDMMDIGIGKPIKVLAERDPKKLPWKAMGIDIVMECTGI
                     FTDRDKAAAHLEAGAKRVLVSAPSKGADLTVVYGVNHDKLKGDHKVVSNASCTTNCLA
                     PVAKVLNDLVGIKSGFMTTIHAYTNDQNVQDQAHKDMRRARAAALSMIPTSTGAASAV
                     GLVLPELKGKLDGTAIRVPTPNVSVIDLKIIPGRETSVEEINKAMEHAAAQSLKGVLA
                     VTKEELVSIDFNHNPASSTFDLTQTQIVDGKMVRVMSWYDNEWGFSNRMSDTAVAMAK
                     LI"
     gene            5915..>6278
                     /locus_tag="PK264_20735"
     CDS             5915..>6278
                     /locus_tag="PK264_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRD78330.1"
                     /translation="MPVFNAAAAARVIADAHARRETYANLSGLLEPPTIADAYAVQEA
                     LLPLWGPTRGPIAGLKIATTTKVMQQLMGIDHPCGGMIYEGTVHSSPHELVLSQHMHV
                     VVECELAVRLDRDLAAKGS"
BASE COUNT         1150 a         2013 c         2098 g         1017 t
ORIGIN      
        1 ttcaacaaca cgatgatcac gatcaccgac gcccagggca acacgatcgc ctggtcctca
       61 tccgggacga agggcttcaa gggctcgcgc aagtcgacgc cgttcgcggc ccagatggcc
      121 gccgaggatg ccggccggaa ggcgcaggag cacggtatga aggtgctcga ggtcgaggtc
      181 tgcggtccgg gctcgggccg cgagtctgcg cttcgcgcgc tgcagtcgat cggcttccag
      241 atcacctcga tccgtgatgt gacaccgatc ccgcacaacg gctgccgccc gcgtaagcgc
      301 cggcgggtct gagacgcgca gcaggctaag cttccggtcg ccctgccgtg ggcggccgtc
      361 tcgcaagcat cgccggcgtc gttcgacgga cgtttggtcc gcccaccgcc ggtattggag
      421 cgcaggagac gcccatgaat cccatgctgc agaagaactg gcaagggcta atcaagccga
      481 ccaagctgca ggtcatgccc ggccgcgatc gcgaccgcgt agcaaccatc gtcgccgagc
      541 cgctcgagcg cggcttcggc atgaccctcg gcaacgccat ccgccgcgtg ctgctttcgt
      601 cgctgcaggg ctcggctgtt tcggccgtgc acatcgacgg tgtgctgcac gagttctcgt
      661 cgatcccggg cgttcgtgag gacgttacga acatcatcct caatatcaag gacatcgcgc
      721 ttcgcattca ttctgatggc gtgaagcgga tggtcttgaa gaaggatagc cgcggcgttg
      781 ccaaggccgg tgacatcgag gcgggctccg acgtcgagat cctcaatcca gagcacgtga
      841 tctgcacgct cgaccagggt gctgcgatcc gcatggagtt cacggcgagc accggcaagg
      901 gttacgtctc ggcggagcgc aacgctccga tcggccttat cccggtcgac agcctgttct
      961 cgccggtgaa gaaggtgtcg tatcgcatcg agaacacgcg cgagggccag atcctcgatt
     1021 acgacaagct gacgatgacg gtcgagacgg acggctcggt gaaggccgag gacgcggtcg
     1081 ccctcgccgc ccgcattctc caggatcagc tcgccgtctt catcaacttc gaggagccga
     1141 agaagcaggt cgaggaggcg aagcaccccg acatcccgtt caacgcggca ctgctcaaga
     1201 aggtggacga gctcgagctc tcggtgcgtt cggccaactg cctgaagaac gacaacatcg
     1261 tctacatcgg cgacctcatc cagaagaccg aggcggagat gttgcgtaca ccgaacttcg
     1321 gccgcaaatc tctcaacgag atcaaggaag tgctggcctc gatgggcttg cacctcggca
     1381 tggaggtccc gaactggccg ccggacaaca tcgaggagct ggcgaagcgg tacgaggacc
     1441 agtactgaga cgtttgcaag gcgggctgcg tgccttgact gcggccggcc tcgcgatctg
     1501 atcccgccgc ctggcgtgcg ggaaacccca gggcgaggcc acagaacctc cccgtgaaca
     1561 acgaaagggt tcgacaatga tgcatggaaa gcgcggccga cgcttcagcc gcgacagtgg
     1621 gcatcgccag gcgatgttct cgaacatggc ggcggcgctg atcaagcacg agcagatcgt
     1681 gacgacgctc gccaaggcca aggatctgcg ccgcgtgatg gacaagtaca tcacgctcgc
     1741 caagcgcggc gacctcaact cgcgccgcct cgcggcctcg cggctgcgtg acgaggacat
     1801 ggtcaagaag ctcttcggcg tgctcggccc gcgttacaag gagcgctcgg gcggctatac
     1861 gcgcgtcctc aaggccggct ttcgttatgg cgatagcgcg ccgcgtgccg tgatcgagct
     1921 ggtcgaccgc gacgtgaccg tgaagggcgc cgaggatcgc gcacgccacg aggccgacgt
     1981 gaaggcgcgc gaagcggcga cggcgccggc cgccgtctga tccgctgaag ccggcggatc
     2041 gttgcagttg ctcaggcggt gcgggcaggc ctcgcgccgc cttatatcta tgatcgcggc
     2101 ccgtgagccg cgtccgaaaa ggagcggcca tgcgcctgcg cttccacacg ctcgatgtgt
     2161 tcacggacca gcgctttgga ggcaatccgc tcgccgtcgt tcacgatgcc gacgcgctcg
     2221 atacgaaggc gatgcaggcc gtcgcgcggg agttcaatct ctccgaaacg gtgttcgtcc
     2281 tgaagcccga gaggccggcg cactcggcaa aggtgcgcat cttcacgccg gccgccgagc
     2341 ttccgttcgc agggcacccg acggtgggca ccgcggccat cctcgccgag ctgcgcgcag
     2401 cgcagacagg gggcggcgcc aatggcgact cgctcgtcgt cctcgagcag cagatcggca
     2461 cggtgcgggt gggggtgcgc atgcgcgccg gcagcgcgcc attcgccgaa ttcgatgcgc
     2521 cccggctgcc agaggaaacg gggcagctcg cgccgatcga ccggctcgcg gcggcgatcg
     2581 gcctcatccc tgcggagatt ggcttcgaga atcaccggcc gacacggttc gcggcgggaa
     2641 atgcctttgc gttcatccct gtcgcgggcc tcgatgcgat cgcgcgggcc gcggtccagg
     2701 cgcagcactg gcagagcgcg ttcggcggcc agggcctcat cggcgcgttc ctctatacgc
     2761 gccagtgcgt gcaggggacc tccgcgttcc acgcgcgcat gttcggcccc gacgtcggcg
     2821 tgatcgagga cccggcgacg gggtccgccg ctgtcggctt tgccggcgcc gtgtatcgct
     2881 tcgacgggat cccggacggc atgcacaagc gcatcgtcga acaaggtttc gagatgggcc
     2941 ggccgagcct gatcacgctt tcgcttacga tcgagcgcgg gcggctcgac acggttcgca
     3001 ttggtggcca gtccgtacgc gtgagcgagg gaacgatcga cgtcagctga gcgcgtctgt
     3061 ggatcagcgg cgatcgtagg tgacgagcgt tgcggcgtac tcatcgcgag gatggggcgg
     3121 gatcagaacg cgcgagacct ctcgccagat cgcgtcgtcg agctccggaa aatgtgtgtc
     3181 cccgtcaggg gcggcgtcga tctcagtcaa gtagatgcga gatgcacgcg ggaatgccgc
     3241 acgataaact tcggccccac cgatgatcat gacctcatcg atgccgttgg tcgcggcacg
     3301 agtcagcgca gaaacaattg cggcatcgaa ggatgcaaac gccgcgacag tcgtgtccgg
     3361 cgcgacctcg atggcgccga tgcctgttct cagggtcggg tcgcgcgtga cgacaagatt
     3421 catccggcca ggaagaggct ttgccaatga ttggaatgtc ttgcggccca tgatcaccgg
     3481 ctttccgagc gtgaggcggc gaaaggtcgc gaggtcggaa gggacacacc acggcagctg
     3541 accgttgcgc ccgatgacac gattgcgagc catggcgacg acgatcgaga tgtcaatgcg
     3601 gcgcgcgccg ggcggtgtcg tcatcgccgg cgcggcggtt gcggcgtacg cacgggcgca
     3661 gccggtcggc tgatcaccgt ggtattcgaa gcgggcgcgg tgacataggg acccggtggg
     3721 tgctcgtgga acgcgctgga cgggtttgca tcgaagcccc gacttggcgg cgggggtggt
     3781 ggcgggggct gatcgacgta agcgcccgaa tacgtgtcgc ccggacggct gttcgcggcc
     3841 ggtggctgag gtgcatacgg tggaaccagg ttcgaggcga tggtctcgac cggcagaggg
     3901 ggcccctgca ggctcgccag ccgggcgcgt agatcgaaca gctcgtcgag cgtcatgatc
     3961 gccgccatca gcatcagacg atcgtcaccg gcatgaccga actcggcgat gatcccgtcg
     4021 acgcgggcgc gcaaatcgtc ggcaagctga atgaggcgcg cctcctcgcc atcgccgcat
     4081 gtcaggcgat aggtgcggcc gttgacggtt atcgtcacct gtcccatgcg cgccgctcca
     4141 ttgggccacg ctcatctggc gctcggccgg cgagcgccga tgtcagcccc tcgcaatctt
     4201 atggagagag tcgagggccg aatcaacccg cgcgaccgcc tcgttgatgc ggccctcgag
     4261 ggcgcgcagg cgcgcattgg ccgccgcgag ctcggatttg agcccttccc gttcgagctc
     4321 gagatcgcgc acgcgctcaa cgaggctctc acgcgataca accgtatcac gcggcgcctc
     4381 gcgcgcggcg gaggccggtg ctcgcggcct cgatttcttg gcgcctcgtt ccgccatgat
     4441 gtgggttcac ccgcttcttt cgggagtgcc ctctccccga attgcgcgcg accttatgtg
     4501 ccgcaggagg agagcgtcaa cacggcacgc gagcgtggtg gaaaaatagg ttggaaacac
     4561 gacgaaaagc gcgccgcagc cacagtctgt cggcttgact cggagaagcc gcgtcaatga
     4621 tgcttcacgc cgcgatatgg cgtgatccct cggcaatgat gtagagggag ccaaggggcg
     4681 cgtccctagt catcgacgac gcgtcgacga ccggcattcg acacactttc gaggaggcaa
     4741 ccgcaatggc agtcaagatc gctatctccg gcttcggccg cattggccgc aacgtgctca
     4801 gggccatcgc cgaatcgaag cgcaacgacg tcgaagtggt cgccatcaac gacttgacct
     4861 cgccggacgt gaatgcccac ctttttcgcc acgacagcgt gcacggcaac tatgcgggca
     4921 ccgtcaagct cgatggcgac atgatggaca tcggcatcgg caagccgatc aaggtgctcg
     4981 ccgagcgcga cccgaagaag ctgccctgga aggccatggg catcgacatc gtgatggagt
     5041 gcacgggcat cttcaccgat cgcgacaagg cggccgccca cctcgaagcc ggcgccaagc
     5101 gcgtgctcgt gtcagcgccg tccaagggcg cggatctgac cgtcgtctat ggcgtcaacc
     5161 acgacaagct gaagggcgac cacaaggtcg tctcgaatgc ctcgtgcacg accaactgcc
     5221 tcgcgcccgt cgccaaggtg ctgaacgacc tcgtcggcat caagtcgggc ttcatgacga
     5281 cgatccacgc ctatacgaac gaccagaacg tgcaggacca ggcgcacaag gacatgcgcc
     5341 gcgcccgcgc cgcggccctc tcgatgatcc cgacctcgac gggtgcggcc tccgccgtcg
     5401 gcctcgttct gccggagctc aagggcaagc tagacggcac tgcgatccgc gtgccgacgc
     5461 cgaacgtgtc ggtgatcgac ctgaagatca tcccgggccg cgaaacgtcg gtcgaggaaa
     5521 tcaacaaggc gatggagcat gccgcggcgc agagcctcaa gggcgtgctt gccgtgacga
     5581 aagaggaact cgtttccatc gacttcaatc acaatccggc atcgtccacc ttcgacctca
     5641 cgcagacgca gatcgtcgac ggcaaaatgg tgcgcgtgat gagctggtat gacaacgagt
     5701 ggggtttctc taaccgcatg agcgacacag ccgtcgccat ggctaagctg atctgagacg
     5761 cgggtccgag gtcgggccga gcacgtcgcc cgtcgatcga cgacttgcga tcgacgggcg
     5821 atcggcggcg tcggccggct gcccagcaga ttgaccaacg ccgcgggggg cgtaatcgcg
     5881 cggccatttg cgacagcgat gacgggaggg tccaatgccg gtattcaatg ctgctgcagc
     5941 cgcgcgtgtg atcgcggatg ctcacgcgcg ccgcgagacc tacgcgaacc tttccggttt
     6001 gctcgagccg ccgaccatcg ccgacgcgta cgccgtgcag gaggccctgc ttccactctg
     6061 ggggccgacg cgcggaccga tcgcgggcct taagatcgcg acgacgacca aggtcatgca
     6121 gcagctcatg ggcattgatc acccgtgcgg tggcatgatc tacgagggca cggtccacag
     6181 cagcccccat gagctcgtgc tgtcgcagca tatgcatgtg gtcgtcgaat gcgagctcgc
     6241 cgtgcggctc gaccgcgatc tggcagccaa aggctccc
//
LOCUS       DAORNH010000277         6261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9260_length_6261_cov_7.168708, whole genome shotgun sequence.
ACCESSION   DAORNH010000277 DAORNH010000000
VERSION     DAORNH010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9260_length_6261_cov_7.168708"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PK264_20740"
     CDS             <1..1186
                     /locus_tag="PK264_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78331.1"
                     /translation="LGRLFERHGDALNGLDLGIFRDEAAEDEARSAVQDFFAGPEENY
                     PEGLVADLHRIAEIGNAAGLDIILQQAARLGIRVTPEAKGGEPEVHQDPKHVALRVFL
                     DHPDVFDAASDMMALMARTSLAEFVGRDEGVEAIMDDRTKAEFETAAAAMFEQDLRSN
                     YCRAGWYDDADELVLVIEHGSPITTTDVLQKDQKRVISFRAAEHAVLSYNSTTGLLKI
                     GGVAKARRGDLAELFADKVLGKPEFFAGDDAQNLYTLDPVQRAGFGFAFNHDFDPGIQ
                     RVQITEVQVDRVGADPKTGETRTFYSYVARDGRDNALARLGEMMRGVRLGSDWRLNHI
                     VIRVHFATGGKSAKKVTIKLKPPAHAMFKRQQFEGRIMTLLRRNGLLNDRDAAQAAVA
                     AE"
     gene            1146..2093
                     /locus_tag="PK264_20745"
     CDS             1146..2093
                     /locus_tag="PK264_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78332.1"
                     /translation="MTETLPRLLLRLSEAGDPAILWGRQAAPHAGRDFERLLDHGVLV
                     EQAPATEWDVCPNCDCGLDARPIHQVNGRHIAVCPTDRRSDVILDDDDLRNFRIHPPA
                     LVREIATASRFGGEPSLVAAGVWHLGETSAQRVLFLALSRDSVLQPGMIGLMRSVARS
                     LPITVIAPAMAADELARFVDAAIAVIPIDGCLGGNAAGFAIDLSTLESAATFEPHLVI
                     FRLSQRVILDGIEMHIPQQPFKLLVMLAEAVGGRSGYLTPHQIEAENSGRGAADLVRE
                     LRRFLSEGRRDRGADLIKTRRSPTGYFLALAPGEFDLRP"
     gene            2223..2798
                     /locus_tag="PK264_20750"
     CDS             2223..2798
                     /locus_tag="PK264_20750"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRD78333.1"
                     /translation="MHSSISQVDLQILLHEADIAARRLVRQLRLPRADLDDVRQDLLV
                     DLIARLPAYDAERGTLGAFAGAILTNRATRIANKAKRERRMYGATPISLDEVIPESDG
                     LTRGDLIAEADGLSALFGQPADAFAAAEHRLDVERGLGSLEPADGALCAALSRTTVDR
                     LAAGGHGARSSLYRRVKDIRLALTAIGVRAA"
     gene            2833..3174
                     /locus_tag="PK264_20755"
     CDS             2833..3174
                     /locus_tag="PK264_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78334.1"
                     /translation="MNVIAPRLPAVRKPLTEIDLCGWVGQAAPGDILEYHRGFLALDT
                     MPQGTRLAERERTELARVARRAWWAAERGLIHLVQRRHRSDDYSYLAIARPKPKLASV
                     SLCSLLLAEAA"
     gene            3174..3668
                     /locus_tag="PK264_20760"
     CDS             3174..3668
                     /locus_tag="PK264_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78335.1"
                     /translation="MASDRNNRPSLDDVRTMPVGEIAKLPAEHLALLQEDADAALDAA
                     KRLKEWLEGAIALRYADAAATVRRAEGKDTGLVRFEDGAVVVVADLPKKIDWDQSLLA
                     ALVERIRAGGENPADYVDIGFKVPERKYTAWPTAIREAFASARTVRTAKPTFRLTIKS
                     EDAR"
     gene            3692..3964
                     /locus_tag="PK264_20765"
     CDS             3692..3964
                     /locus_tag="PK264_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78336.1"
                     /translation="MRKRHYALEALPDTVVIPVLGEIRREQVVKPIEDATLDDIAFAL
                     LGVEAEFSAVGDRLHALRKLYGLARQAGARGSERALDVVSRDAGGR"
     gene            3964..4797
                     /locus_tag="PK264_20770"
     CDS             3964..4797
                     /locus_tag="PK264_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD78337.1"
                     /translation="MGLRIVSADERLSAAGAKTTMAIFGPSGVGKTSLLKSLPPAETL
                     CIDLEAGMKSVQDWPGDSIPVRTFADALDIGCLVGGVNPSADPNGFFSEAHYQHLRES
                     YPDLVQMIAGKRIIFVDSITDLTRQAMAWAKTRPEAFSDKTGKPDTRGAYGLLAREVI
                     GLLKHLQHAQAKTVIFVGILERVTDEFNRTAWQPQMEGGKAGRELPGIVDQVITMSLF
                     AADGDGWRHEPERGEVRRLVCRAGNPFALPAKDRSGRLDVTEPPDLGALLSKINATRK
                     G"
     gene            4804..5541
                     /locus_tag="PK264_20775"
     CDS             4804..5541
                     /locus_tag="PK264_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78338.1"
                     /translation="MSFDMNDAEPQKSGELIPDGTFAKITMTIRPGGTDGQSEIDRGL
                     LKASNTPGSDVLMVDAEFTVAEGPHVRRKFWQMFTVSGGKVDEHGVSIGWKISKGSFR
                     AMIDSALGLDPSDMSDATKAKRILRGLADLNGITFVAKIKVEPSDDPRYGDSNKLDRV
                     VLPSEPEWRKVMDGEVLAPSPSTRARPKAVVPVSHAAPAWGQAAVKAPASTAPAWNRT
                     VAPGAPPPAASSSTPKPGMTGPAWLNT"
     gene            5547..5774
                     /locus_tag="PK264_20780"
     CDS             5547..5774
                     /locus_tag="PK264_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78339.1"
                     /translation="MTADEWQAHVTREAAKAMGQWLEGRGRLHQPIAALTLADLEAMA
                     ANAIARFIVLASHRIKDQPDDAEDLTRLLLG"
     gene            5915..6130
                     /locus_tag="PK264_20785"
     CDS             5915..6130
                     /locus_tag="PK264_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007426738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6511 domain-containing protein"
                     /protein_id="HRD78340.1"
                     /translation="MIDKTDMETRAIKEARRFLAETLTELDLMAPFYDRKPEEIDRII
                     EACVDGFQESMQRQAAARDPLDDPIPF"
BASE COUNT         1104 a         2137 c         2009 g         1011 t
ORIGIN      
        1 gctgggccgc ctgttcgagc gccacggcga tgccctgaac gggctggacc ttgggatctt
       61 cagggacgag gcagcggagg acgaggcacg cagcgccgtc caggatttct tcgccggccc
      121 ggaggagaac tacccggaag gactcgtcgc cgacctgcac cggatcgcgg agatcggcaa
      181 cgcggcaggc ctcgacatca tccttcagca ggccgcgcgg ctcgggatac gcgtgacacc
      241 cgaagcgaag ggtggcgagc cggaggttca ccaggatccc aagcacgtcg ccttgcgcgt
      301 cttcctcgac catcccgatg tctttgacgc cgcgtcggac atgatggcgc tcatggcgcg
      361 cacgtcgctc gccgagttcg tcggtcgcga cgaaggcgtc gaagcgatca tggatgatcg
      421 cacaaaggcc gagttcgaaa ccgccgccgc agcgatgttc gaacaggacc tccgcagcaa
      481 ctattgccgc gccggctggt atgacgatgc cgacgaactc gtcctggtga tcgagcacgg
      541 gtctccgatc accacgaccg atgtgctgca aaaggaccag aagcgcgtaa tcagcttccg
      601 cgccgccgaa cacgcggtgc tgtcctacaa ctccacaacg ggcctattga agatcggcgg
      661 cgtcgccaag gcgcgtcgcg gcgacctcgc ggaactgttc gccgacaagg tcctgggcaa
      721 gcccgaattc ttcgccggcg acgacgccca gaacctctac acgctcgacc cggtgcaacg
      781 cgcgggcttc ggcttcgcgt tcaaccatga cttcgatccg ggcatccagc gggtccagat
      841 caccgaggtc caggtcgacc gcgtcggcgc cgatccgaag accggcgaga caagaacctt
      901 ctactcctat gttgcgcgcg acggacgcga caatgccctg gccaggttgg gcgagatgat
      961 gcggggcgtt cgtctcgggt cggattggcg cctcaaccac atcgtcatcc gcgtccattt
     1021 cgcgaccggc ggcaagtcgg cgaagaaggt gaccatcaag ctgaagccgc cggcacacgc
     1081 catgttcaag cgtcagcagt tcgagggccg gatcatgacg ctccttcgcc gcaacggact
     1141 gctcaatgac cgagacgctg cccaggctgc tgttgcggct gagtgaggcg ggcgatcccg
     1201 caattctttg gggccgacag gcggcgcctc atgccgggcg agatttcgag cggctgctcg
     1261 atcatggcgt cctggtcgag caggcgccgg caacggaatg ggatgtgtgc cccaactgcg
     1321 attgcggact cgacgcgcgg cccatccatc aggtcaacgg ccgacatatc gcggtttgcc
     1381 cgacggaccg gcgcagcgat gtcattctcg acgatgacga cctacggaac tttcgcattc
     1441 atcctcccgc gctggtccgc gaaatcgcca ccgcatccag attcggcggc gagccgtccc
     1501 tggtcgcggc gggcgtctgg catctggggg agacgtcggc ccagcgggtg ctgttcctcg
     1561 cactgtcgcg ggattcagtg cttcaaccgg gaatgatagg cctgatgcgt tcggtcgccc
     1621 ggtcgttgcc catcacggtg attgcgccgg cgatggcggc ggacgaactc gcccgcttcg
     1681 tcgacgctgc gatcgccgtc ataccgattg acggctgtct gggcgggaac gcggcgggct
     1741 tcgcgatcga cctttcgacg ctggaatccg ctgcgacctt cgaaccgcat ctggtcattt
     1801 tccgtctgag ccagcgggtg atcctggatg gcatcgagat gcacatcccg cagcagccgt
     1861 tcaagcttct cgtgatgctg gccgaggctg tcgggggtcg gagcggctat ctgaccccgc
     1921 atcagataga ggccgaaaac agcgggcgag gcgctgctga tctcgttcgc gaattgagac
     1981 gtttcctgtc cgagggacga cgggatcgtg gtgcagacct gatcaagacg cgacgaagcc
     2041 cgaccggcta cttccttgcg ctggctccgg gagagtttga tctgcggcca taggagcgct
     2101 ggcccgcatc accgctcgtc ttcccattct cgcccatcca aaacccatcc cgctcccacc
     2161 taacggatcg gcggcttcgg caggcttgag gtcatcaaca gcgatgaccc gaggccatgc
     2221 caatgcactc ttcgatttcc caagtcgatc ttcaaatcct tcttcatgag gcggacatcg
     2281 ccgcccgccg cctcgtccgc cagctgcggc ttcctcgcgc cgatctcgac gatgtccgcc
     2341 aggacctgct cgtcgacctg atcgcgcgtc ttcccgccta cgacgctgaa cgcggcaccc
     2401 tcggcgcctt tgccggcgcc atcctcacca acagggcgac gcgcatcgcc aacaaggcga
     2461 agcgcgagcg ccgcatgtac ggcgcgacgc cgatctcgct cgatgaggtc attcccgaga
     2521 gcgacgggct gacccgcggc gacctcatcg ccgaagccga cgggctgtcg gcactcttcg
     2581 ggcagcccgc cgatgcattc gccgccgccg agcaccgtct cgatgtcgaa cgcggtcttg
     2641 gctcgctcga acccgccgat ggcgctctct gcgcggccct gtcccgcacc accgtcgacc
     2701 gccttgccgc aggcggccat ggcgcccgca gcagcctcta tcggcgcgtg aaggacatcc
     2761 gccttgccct gacagcgatc ggcgtgcggg ccgcctgaga cggttcggcg agtgcgtgag
     2821 taggagccaa acatgaacgt cattgcaccc agactcccag ctgtccggaa gccgctcacc
     2881 gagatcgatc tctgtggctg ggtcggccag gctgcgcccg gcgacattct cgaatatcac
     2941 cgtggcttcc tcgcgctcga caccatgccg cagggcacgc gccttgccga gcgggagcgg
     3001 acggaactcg cgcgtgtcgc gcgccgcgcc tggtgggcgg ccgagcgcgg actgatccat
     3061 ctcgtgcagc gtcgccaccg gtcggacgat tacagctatc tcgccatcgc ccgcccgaag
     3121 ccgaagctgg cctcggtctc gctgtgctcg ctcctgctgg cggaggcggc gtgatggcat
     3181 ctgatcgcaa caaccgcccc agcctcgacg acgtccgcac catgccggtc ggcgagatcg
     3241 ccaagcttcc ggcggagcat ctggcgctgc tccaggagga tgccgacgca gccctggatg
     3301 ccgcgaagcg gcttaaggag tggctcgaag gtgcgatcgc gcttcgctac gccgacgcgg
     3361 cagcgacggt gcgccgggcc gagggcaagg acactggcct cgttcgtttc gaggacggcg
     3421 ccgtcgtcgt cgttgccgat ctcccgaaga agatcgactg ggaccaatcg ctgctcgccg
     3481 ccctcgtcga acggatccgt gcaggcgggg agaacccggc cgactacgtc gacatcggct
     3541 tcaaggtccc cgagcgcaaa tacaccgcct ggcccaccgc catccgcgag gccttcgcct
     3601 ccgcccgaac ggtgcggacg gccaagccga ccttccgtct caccatcaag tccgaggatg
     3661 cccgatgatc agctctgctg ccctgaccga gatgcgcaag cgccactacg cgctcgaagc
     3721 gctgcccgat accgtcgtca ttccggtgct aggcgagatc cgccgcgagc aggtggtcaa
     3781 gccgatcgag gacgctacgc tcgacgatat cgccttcgcc ctgctgggcg tcgaagccga
     3841 gttcagcgcc gtcggcgacc gtcttcacgc cttgcgtaaa ctctacggtc tcgcccggca
     3901 agccggcgcg cgcgggagcg aacgcgcgct cgatgtcgtg tcacgcgacg caggaggccg
     3961 ctgatggggc tgcgcatcgt aagcgccgac gaacggctgt ccgcggccgg tgccaagacc
     4021 accatggcaa tcttcggccc gagcggcgtc ggcaagacct ccttgctgaa atcgctgcca
     4081 ccggccgaga cgctctgcat cgatctcgag gcgggaatga agtcggtcca ggattggccc
     4141 ggcgatagta tcccggtgcg cactttcgca gacgcgctcg acatcggctg cctcgtcggc
     4201 ggggtcaatc catcggccga tccaaacggc ttcttctccg aggcgcatta ccagcatctc
     4261 agggaaagct atcccgatct cgttcagatg atcgcgggca agcgcatcat cttcgtcgac
     4321 agcattaccg atctcacgcg ccaggccatg gcctgggcga agacccggcc cgaggccttc
     4381 tccgacaaga ccggcaaacc ggacacccgc ggcgcctacg ggctcctcgc ccgcgaggtc
     4441 atcggcctgc tcaagcacct gcagcacgcg caggcgaaga cggtgatctt cgtcggcatc
     4501 ctcgaacgcg tcaccgacga gttcaaccgc acggcctggc agccgcagat ggaaggcggc
     4561 aaggccggcc gcgagctccc cggcatcgtc gatcaggtca tcaccatgag cctgttcgcg
     4621 gccgatgggg acggctggcg gcacgagccg gaacgcggcg aggtccgccg cctcgtctgc
     4681 cgcgccggca atcccttcgc cctgccggcg aaggatcgca gcggtcgtct cgatgtcacg
     4741 gagccgcccg acctcggcgc gctgctctcc aagatcaacg caacccggaa aggatgacga
     4801 gccatgagct tcgacatgaa tgacgccgag ccgcagaaga gcggcgaact gatccccgac
     4861 ggcaccttcg ccaagatcac catgaccatt cggccgggcg ggacagacgg ccagagcgag
     4921 atcgaccggg ggctgctcaa agcgtcgaac acgcccggca gcgacgtgct gatggtcgat
     4981 gccgagttca ccgtcgccga gggtccgcac gtccggcgca agttctggca gatgttcacc
     5041 gtctcgggcg gcaaggtcga cgagcacggc gtctccatcg gctggaagat ctccaaaggc
     5101 agcttccgcg cgatgatcga cagcgcgctc gggctcgatc ccagcgacat gagcgatgcc
     5161 accaaggcaa agcgcatcct gcgcggtctg gccgatctca acggcatcac cttcgtcgcc
     5221 aagatcaagg tcgagcccag cgacgacccg cgctacggcg acagcaacaa gctcgaccgt
     5281 gtggttctgc cgagcgagcc agagtggcgc aaggtgatgg acggcgaggt cctagcgccg
     5341 agccccagca cccgtgcgcg gccaaaggca gtggtgccgg tgtcgcacgc ggctcctgcg
     5401 tggggacagg ccgcggtgaa agcgccagca agtaccgcgc ccgcctggaa ccggacggtg
     5461 gcgccgggtg cgcccccgcc cgcggcttcg tcgtcaacgc cgaagcctgg catgacagga
     5521 ccggcctggc tcaacacctg accgtcatga cggccgatga gtggcaggcg catgtgacgc
     5581 gcgaggcggc gaaggcgatg ggacaatggc tcgaaggacg cggaaggctt caccagccga
     5641 tcgccgctct cacactggcc gacctggagg cgatggcggc gaacgcgatc gcgcggttca
     5701 tcgtcctggc ctcacaccgg atcaaggatc agccggacga cgcagaggac ctgacccggc
     5761 tcttgctcgg gtaggcgtct gcgccgtctg cggacgcgag gcgcggggct tcggctacgt
     5821 ccaccagctg cgctgggacc gctttcctta tcaccgcttc tgctcgatgc gctgcctcga
     5881 tgtcggctcg gcgctcgcca acaggaacta cgggatgatc gacaagaccg acatggagac
     5941 ccgagcaatc aaggaggcgc gccggtttct cgccgagacg ctcaccgagc tcgacctgat
     6001 ggcaccgttc tacgaccgga agccggaaga gatcgaccgc atcatcgagg cctgcgtcga
     6061 cgggtttcag gagtcgatgc agcgtcaggc ggccgcccgc gatcctctcg acgatccgat
     6121 tccgttttga ggagcacatg gggatcgatc tcaaccacgg ctccggcttc gtctatggcc
     6181 gcttaggcga tgcgatcggc gtgtctgatc gggtcaatgc gctgatcgac gaggctctcg
     6241 tggcgcgcaa ccggcgtcaa a
//
LOCUS       DAORNH010000278         6259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9265_length_6259_cov_2.486299, whole genome shotgun sequence.
ACCESSION   DAORNH010000278 DAORNH010000000
VERSION     DAORNH010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9265_length_6259_cov_2.486299"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PK264_20790"
     CDS             complement(<1..883)
                     /locus_tag="PK264_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HRD78341.1"
                     /translation="MSTVRRSAVGRFIGAFAVATATLAAPALAADCGNDGAGFDAWLG
                     RFKGRAAAQGITAQTIAIGLREASYDPNIIRLDRNQKSFKLTFEEFYARRVSTSLINR
                     GRRLMATHKATLDRVEARYGVPAAVLVSIWGLETNYGADGGGGRSIVRSIATLAYDCR
                     RTDFFQDQLMSVLKIIQRGDIPVEMLRGGWAGEIGQVQFLPTPYFKYAVDFDGDGSRD
                     LFRSVPDMMASVANFLKSHGWQPRQPWGEGTANYNVIRDWNKAEVYVKTIATMSARMG
                     EAGPPPPAAPRAPRPAKT"
     assembly_gap    892..914
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1010..2611)
                     /locus_tag="PK264_20795"
     CDS             complement(1010..2611)
                     /locus_tag="PK264_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRD78342.1"
                     /translation="MPDVVRTLVAALMLLATGIAGLPSGALAQAGVYHRGNDGDPETL
                     DPHKTSTVSEAHLLRDLFEGLVIHDGLGRVVPGVAERWEITPDGRLYRFHLRANAKWS
                     NGEAVKAGDFVYSLRRIMAPDTGAKYANILYPIQGAEKIARREAAPETLGVKAVDDRT
                     LEITLERATPYFLELLTHQTGLPVHPGNVTAAGKDFVRPGTLVSNGAYRLVENVPNSH
                     ILLEKNQHFHDAANVRIPAVRYHPIKDLAAAARRFMAGELHSTSDFPADQYKFLKDKL
                     GSQVVVAPYLGTYYLAFNTERTPFGDVRVRTALSMAIDREFLNDYIFSATMVPAYGFI
                     PPGIGNYGAPAEMSFKALAPIEREERARQLLADAGYGPKKPLKVEIRYNITDNNRNAM
                     VAIADMWKSIGVETTFISTDAKTHFAYLRDGGAFDVARAGWIGDYSDPQNFLFLVQSD
                     NPGFNYARYKNPEFDVLMKEAGAETDLAKRALILLAADRMFVRDQPFAPLLFYSSRHL
                     ISPKLKGLTPNLRGANATRFMSLEP"
     gene            2726..3439
                     /gene="sfsA"
                     /locus_tag="PK264_20800"
     CDS             2726..3439
                     /gene="sfsA"
                     /locus_tag="PK264_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HRD78343.1"
                     /translation="MRFTKPLIPATLVKRYKRFLADVRLGDGTALTATCPNTGSMLGL
                     TDPGLKVWISHSESPTRKYAHTLELIEVDLGKGPGLVGINTGHPNKLVAEAIEAGRVK
                     ELKGYESLRREVKYGENSRIDLLLEGGGKSACYVEVKNVHMMRKAGLAEFPDSVTERG
                     AKHLREMSEMVRQGHRAVMVFLIQRSDAKSLTLASDIDPGYLEAFTAAKAAGVEAIAL
                     RCSISPDEIAVDKAVPIKS"
     gene            complement(3449..>4205)
                     /locus_tag="PK264_20805"
     CDS             complement(3449..>4205)
                     /locus_tag="PK264_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD78344.1"
                     /translation="GPAETMTAQMILSAALILPLIVWTGPPPAAAWPWMLLSTSLNVL
                     TVGALLKAYELAGFGVGYAVVRAISVLLVVPLMAVIAGERIGATGLVGVALIAASLGM
                     LGLGARREDGLAPAAMLWMLIAGLGTAGYVVADAQGVRAAGSALAYGIVVSTTNALAM
                     SWRRRADGSPLAILQRHGLRALPIAMASAASYLLILWVWTSAPVAPTAALRDTSAVFG
                     VIIAVLVLKERLTAWRLAAVALAALAVPFLRLG"
     assembly_gap    4206..4254
                     /estimated_length=49
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4319..5122)
                     /locus_tag="PK264_20810"
     CDS             complement(4319..5122)
                     /locus_tag="PK264_20810"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRD78345.1"
                     /translation="MSTWQVFFLGLLEGLTEFIPVSSTGHLLLAGHFLGFKSPGKSFE
                     ILIQLGAVLAVVTVYFGRLWQLFVTLPSSAESRRFVLGILLAFLPAALVGVVAHKFIK
                     SVLFETPILICVMLIVGGIILLIVDRMPLETRYHDVTQLPPLLALKIGLAQCLAMIPG
                     VSRSGATIVGALLMGVEKRAAAEFSFFLAMPTMAGAFTYDLMKNWSALSAADVKSIGI
                     GFVVAFLAGVVVVRYLLAFVSRNGFAPFAWWRIIAGGLGLGALLATGLA"
     gene            complement(5238..5480)
                     /locus_tag="PK264_20815"
     CDS             complement(5238..5480)
                     /locus_tag="PK264_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HRD78346.1"
                     /translation="MSSEPKVAQKGPYQVELQEGKAYFWCACGLSKRQPYCDGSHKGS
                     GIEPVKFVAESTGTFNLCGCKMSDDKPFCDGQHNVL"
     gene            complement(5592..5996)
                     /gene="mce"
                     /locus_tag="PK264_20820"
     CDS             complement(5592..5996)
                     /gene="mce"
                     /locus_tag="PK264_20820"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRD78347.1"
                     /translation="MIGRLNHVAIAVKNLASSTAVYRATLGAEVTAPLPQPEHGVTVV
                     FVNLPNTKIELLEPLGEGSPIAKFLERNPDGGIHHICYEVDDIIAARDKLKGDGARVL
                     GSGEPRIGAHGKPVLFLHPKDFCGTLVELEQA"
BASE COUNT         1100 a         1899 c         2192 g          996 t
ORIGIN      
        1 cggtcttcgc gggacgcggg gcgcgcggcg cagccggtgg cggcggaccc gcctcgccca
       61 tgcgcgccga catggtggca atggtcttca catagacctc cgccttgttc cagtcgcgga
      121 tcacgttgta gttcgcggtg ccctcgcccc agggctggcg cggctgccag ccgtgcgact
      181 tgaggaagtt ggcaaccgag gccatcatgt cgggcacgct gcggaagagg tcgcggctgc
      241 cgtcgccgtc gaagtcgaca gcatacttga agtagggcgt cggcaggaac tgcacctggc
      301 cgatctcgcc ggcccagccc ccgcgcagca tctcgacggg aatgtcgccg cgctggatga
      361 tcttcaggac gctcatcagc tgatcctgga agaaatcggt gcgccggcaa tcgtaggcaa
      421 gcgtcgcgat cgagcgcacg atcgacctgc cgccgccgcc gtcggcgccg tagttggtct
      481 caagacccca gatcgagaca agcaccgccg cgggcacacc atatcgcgcc tcgacgcggt
      541 cgagtgtcgc cttgtgggtc gccatcaggc gtcggccgcg attgatgagc gaggtcgaga
      601 cgcggcgcgc gtagaactcc tcgaaggtga gcttgaagga cttctggttg cggtcgagcc
      661 ggatgatgtt cggatcgtac gaggcttcgc ggaggccgat ggcgatggtc tgggccgtga
      721 tgccctgggc cgcagcgcgt cccttgaatc ggccgagcca ggcgtcgaag cccgcgccgt
      781 cgttgccgca gtcggcggca agtgcggggg cggcaagggt ggcggtcgcg acggcaaagg
      841 cgccgatgaa gcgcccgacg gcagatcgtc tcacagtgct catgcttcaa cnnnnnnnnn
      901 nnnnnnnnnn nnnnaccgaa cggcggaagc cgttgatcag ggcttgtttc cgggcggcta
      961 tagtaggccg ccgatcgact gtcagtccaa tcgcgcgatg ccggtccggc tagggctcga
     1021 gcgacatgaa ccgcgtggca ttggcgcccc gcaggttcgg cgtcaatcct ttgagcttcg
     1081 gcgagatgag gtgccggctc gaatagaaga ggagcggcgc gaacggctgg tcgcgcacaa
     1141 acatgcggtc tgccgcgagc aggatgaggg cccgcttggc gagatcggtc tcggcgccgg
     1201 cctccttcat gagcacatcg aactccgggt tcttgtagcg cgcgtagttg aagcccgggt
     1261 tgtcggactg cacgaggaac aggaagttct gcgggtccga gtagtcaccg atccagccgg
     1321 cgcgcgcgac atcgaaggcg ccgccgtcgc gcagatacgc gaagtgggtt ttcgcgtccg
     1381 tcgagatgaa ggtcgtctcg acgccgatcg acttccacat gtcggcgatg gctaccatcg
     1441 cgttgcggtt gttgtcggtg atgttgtagc ggatctcgac cttcagcggt ttctttgggc
     1501 cgtagccagc atcggccagc agctggcgcg cgcgctcctc gcgctcgatg ggcgcgagcg
     1561 ccttgaagct catctcggcg ggagcgccat aattgccgat gcctgggggg atgaagccgt
     1621 aagccggcac catggtggcg gagaagatgt agtcgttcag gaactcgcga tcgatcgcca
     1681 tcgagagagc ggttctcacc cgtacgtcgc cgaagggtgt ccgctcggta ttgaaggcga
     1741 ggtagtaggt gccgagatag ggggcgacga ccacctgcga gccgagcttg tccttgagaa
     1801 acttgtattg gtcggccggg aagtcgctcg tcgaatggag ttcgcccgcc atgaagcggc
     1861 gcgcggcggc ggcgagatcc tttatcggat ggtagcggac ggccggaatg cgtacgtttg
     1921 ctgcgtcgtg gaaatgctgg ttcttctcga gcaggatgtg cgagttcggc acgttctcga
     1981 cgaggcgata ggcgccgttc gagacgagcg tgccggggcg cacgaagtcc ttgcccgccg
     2041 cggtcacatt gcccggatgg acgggaagcc ccgtctggtg ggtcagcaat tcgaggaagt
     2101 aaggggttgc ccgctcgagc gtgatctcga gtgtgcggtc gtccacggcc ttgacgccga
     2161 gcgtctcggg tgcggcctcg cggcgcgcga tcttctcggc gccctggatc ggatagagga
     2221 tgttggcgta cttggcgccc gtatccggcg ccatgatgcg gcggagcgag tacacgaagt
     2281 cgccggcctt gacggcctcc ccgttcgacc actttgcatt ggcgcggagg tgaaagcggt
     2341 agaggcggcc gtcgggggtg atctcccaac gctcggcgac gcccggcact acgcggccga
     2401 gaccgtcatg gatgacgagg ccctcgaaca ggtcacggag cagatgcgcc tcggagacgg
     2461 tcgaggtctt gtggggatcg agcgtctcgg gatcgccgtc gttgccgcga tgatagacgc
     2521 ccgcctgcgc gagcgcgccc gacggcagtc cggcgatccc tgtcgcgagc agcatcaggg
     2581 ctgccacaag cgtgcgcacg acgtccggca tgcaaggctc cactgccgac ccaatttcaa
     2641 atcgtgccac cttcatgttg aaaggtgtcc gcagcggggc agagcggcga ttgcgcgtgg
     2701 cagcagggag agctacctct tgcccatgcg attcacgaag cccctcatcc cggcgacgct
     2761 cgtcaagcgc tacaagcgct tcctcgccga tgtccgcctc ggtgacggca cggccttgac
     2821 cgcgacctgt ccaaacaccg gctcgatgct cggcctcacc gatcccgggc tcaaggtgtg
     2881 gatttcgcac tcggagagcc cgacgcgcaa gtatgcccac accctcgagc tgatcgaggt
     2941 cgatctcggc aaggggccgg ggctcgtcgg catcaatacc gggcatccga acaagctcgt
     3001 cgcggaggcg atcgaggcgg ggcgcgtcaa ggagctcaag ggctacgaga gcctcaggcg
     3061 cgaggtgaag tacggcgaga acagccgcat cgatctgctg ctcgaaggcg gcggcaagtc
     3121 cgcctgctac gtcgaggtga agaacgtcca catgatgcgc aaggccgggc tcgccgagtt
     3181 cccggacagc gtcaccgagc gcggcgccaa gcatctgcgc gagatgtcgg agatggtgcg
     3241 gcagggccat cgcgccgtga tggtgttcct gatccagcgg tccgacgcga agagcctaac
     3301 gctcgcgagc gatattgatc cgggctatct cgaggccttc acggcggcca aggccgccgg
     3361 cgtcgaggcg attgcgcttc gatgcagcat ctcgcccgac gagattgcgg tcgacaaggc
     3421 cgtgccgatc aagtcgtagc atgggggttc agccgagcct gaggaacggc acggcaagcg
     3481 ccgcgagcgc gacggcggcg agcctccatg cggtcaatcg ctccttcagg acgagtacgg
     3541 cgatgatgac gccgaaaacc gcgctcgtat cgcggagcgc agcggtaggc gccaccggtg
     3601 ccgaggtcca cacccacagg atcagcaggt aggacgctgc ggacgccatg gcgatcggca
     3661 gtgcgcggag cccgtgccgc tgcaggatgg cgagcggcga gccgtcggcg cggcgtcgcc
     3721 acgacatggc gagcgcattg gtcgtcgaga cgacgatgcc gtaggcgagt gccgagcccg
     3781 cggcgcgcac gccctgcgcg tcggccacca catagcctgc ggtgcctagg cccgcgatca
     3841 gcatccagag catcgctgcc ggagcgagtc cgtcctcgcg ccgtgcgccg aggccgagca
     3901 tgccgagcga ggcagcgatc aaggcgacgc cgacgagccc cgtggcgccg atgcgctcgc
     3961 ccgcgatcac tgccatcagc ggcacgacaa gcagcaccga gatcgcccgc accacggcat
     4021 agccgacacc gaagcccgcg agctcatagg ccttgaggag cgcgccgacc gtcagcacgt
     4081 tgagcgaggt cgagaggagc atccagggcc aagccgctgc gggcggcggt ccggtccata
     4141 cgatgagcgg cagaatgagc gcggcgctca ggatcatctg cgcggtcatg gtttcggcgg
     4201 gacccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcgat
     4261 cagatgccac gtctccatga gagcctctca gcgtcggagc ccgaataccg gctcccgatc
     4321 aggcgaggcc cgttgcgagc aaggcgccga gaccgagccc gccggcgatg atgcgccacc
     4381 aggcgaacgg cgcgaagcca tttcgcgaga cgaacgcaag aaggtatcgc accacgacca
     4441 cgcccgcgag gaacgcgacg acgaagccga tgccgatgct cttcacgtcg gccgcggaga
     4501 gagccgacca gttcttcatg aggtcatagg tgaaggcgcc ggccatggtc ggcatggcga
     4561 ggaagaacga aaactcagca gcggcacgct tctcgacgcc catcagcagc gcgccgacga
     4621 tcgtggcgcc tgaacgcgag acgcccggga tcatggcgag gcactgggcg aggccgatct
     4681 tcagggcgag cagcggcggc agctgggtga catcgtgata gcgcgtttcg agcggcatgc
     4741 ggtcgacgat cagcaggatg atgccgccga cgatcagcat gacgcagatg aggatcggcg
     4801 tctcgaacag caccgacttg atgaatttgt gagcgacgac gccgacgagc gccgctggca
     4861 ggaaggcgag caggatgccg aggacgaagc ggcgactctc ggcgctcgac ggaagggtca
     4921 cgaaaagttg ccagagccgg ccgaagtaga cagtgacaac ggcgaggacc gcgccaagct
     4981 ggatcaggat ctcgaacgac ttcccgggcg acttgaaccc caggaaatgc ccggcgagca
     5041 gcaggtgccc ggtggacgat accgggatga attcagtgag cccttcgagg agacccagga
     5101 aaaagacctg ccaagtcgac atattccggc ctccgggcca tcgatgggct gtgctggcac
     5161 cggtttaggc aggggcaggc ggattcgcga acgcgccgtg atgtctcact tgatggcggc
     5221 tcgagccggg cgcgtcgtca cagcacgttg tgctgcccgt cgcaaaacgg cttgtcgtcg
     5281 gacatcttgc agccgcagag gttgaaggtg ccggtgctct cggcgacgaa cttcacgggc
     5341 tcgatccccg agcccttgtg cgagccgtcg caatagggct ggcgcttgga gaggccgcag
     5401 gcgcaccaga agtaggcttt gccctcctgc agctccactt gataggggcc cttctgggct
     5461 actttcggct cgctcgacat aggtcctcgc tcgtctcgcg tcatggcgca cggtcagcgg
     5521 cagagaatag ggtgagtggc gcccaaactg caatggcggt catcatggca ctgatcgggg
     5581 cctgaacgag ctcaggcctg ctcgagctcc accagagttc cgcagaagtc cttggggtgc
     5641 aggaagagca cgggcttgcc gtgggcgccg atgcgcggct cgccgctgcc gaggacgcgc
     5701 gcgccgtcac ccttcagctt gtcgcgggcg gcgatgatgt cgtcgacctc gtagcagatg
     5761 tgatggatgc cgccgtcagg attgcgctcg aggaacttgg cgatcggtga gccctcgccg
     5821 agtggttcca gcagctcaat cttggtgttc ggcaggttga cgaagacgac cgtcacgccg
     5881 tgctccggct ggggcagggg cgcggtcacc tcggcaccga gcgtcgcgcg gtagacggcc
     5941 gtgctcgacg ccagattctt gacggcgatg gcgacgtgat tgaggcgtcc gatcatggcg
     6001 ggaggtctcg cggttggcgg tcgcggctcg acacgagcaa cgggaggatc gtgaagagct
     6061 ggtctagctc aagccttagt ggctcacggg ccggtaagct atcgtttcca gcgcgcgggc
     6121 tgtccattgc gaccttcgtc ggcggcagag cgggaaaggg cgcagcgccg ctcgggcgcg
     6181 ttacggcttc aggcgggtgg agacggcatc ggccggtgtc gtgccatcct cgggggcgct
     6241 gtcgcgtgag gttgcagag
//
LOCUS       DAORNH010000279         6256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9274_length_6256_cov_2.983712, whole genome shotgun sequence.
ACCESSION   DAORNH010000279 DAORNH010000000
VERSION     DAORNH010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9274_length_6256_cov_2.983712"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PK264_20825"
     CDS             complement(<1..568)
                     /locus_tag="PK264_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRD78348.1"
                     /translation="MVSAAEQLASNLNFGAFSKATELKRRLLFTVLALLVYRLGTYIP
                     IPGVNPAAFAQQFQNNANNILGMLNMFAGGAVERMAIFALNIMPYISASIIMQLMSSV
                     SPRLEALKKEGEQGRKQINQYTRYLTVILATFQAYGISLGLEGQTGGQAGNVVLEPGF
                     FFRMSTVITLVGGTMFLMWLGEQITQRGV"
     gene            complement(707..1258)
                     /gene="rplO"
                     /locus_tag="PK264_20830"
     CDS             complement(707..1258)
                     /gene="rplO"
                     /locus_tag="PK264_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRD78349.1"
                     /translation="MRLNGMKDNPGSRKERVRIGRGIGSGMGKMGGRGGKGQTARTGV
                     RLGGFEGGQMPLFRRLPKRGFNTMDRRRFNEMNVGALQKAIDEGRLDATKPVDNAALV
                     AAGLVRRAHDGLRLLGDGELKAKLSITVDHATASAKAAVEKAGGSVTVIERKVLPADE
                     AKRAKSAAKKAKQVKKGGGAAED"
     gene            complement(1291..1500)
                     /gene="rpmD"
                     /locus_tag="PK264_20835"
     CDS             complement(1291..1500)
                     /gene="rpmD"
                     /locus_tag="PK264_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRD78350.1"
                     /translation="MTTKKPGGKTVTIEQYASPNRRPEVQRQTLIGLKLNKIRRRSTL
                     EDTPAVRGMIDSLPHLVRVVDDKKA"
     gene            complement(1522..2118)
                     /gene="rpsE"
                     /locus_tag="PK264_20840"
     CDS             complement(1522..2118)
                     /gene="rpsE"
                     /locus_tag="PK264_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRD78351.1"
                     /translation="MARNPSRDRDRDRDDRDNEFAEKLVHINRVTKVVKGGKRFSFAA
                     LVVVGDKQGRVGFGHGKAREVPEAIRKATEGARRALIRVPLRDKRTLHHDSVGHHGAG
                     KVTLRSAPPGTGIIAGGRVRAVLEMLGVQDVVSKSTGSTNAYNMVRATFDALKHQENP
                     RAVAARRGKKVSEIISRRRDQAGQGEAAQTEAGGPDAA"
     gene            complement(2135..2497)
                     /gene="rplR"
                     /locus_tag="PK264_20845"
     CDS             complement(2135..2497)
                     /gene="rplR"
                     /locus_tag="PK264_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRD78352.1"
                     /translation="MSAHDSQFQRRRARVRRTLKKVAAGRPRLSVHRSHLHIYAQVID
                     DAKGLTLASASTIEKDVRGRIKSGGNKSAATEVGKMIAERAIKAGVKEVTFDRGAYKF
                     HGRVKALADAARAGGLSF"
     gene            complement(2513..3052)
                     /gene="rplF"
                     /locus_tag="PK264_20850"
     CDS             complement(2513..3052)
                     /gene="rplF"
                     /locus_tag="PK264_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004685699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRD78353.1"
                     /translation="MSRIGKRPVPVPANVQATVAGQKVTLKGPKGELALDMPEELKVE
                     KTAAGIALTPREDSKTARSMWGMSRTLIANNIVGVQNGYSRTLEIHGVGYRAQMKGKD
                     LNLQVGFASDITYKIPAGVEVKIAGAKQEQIQITGIDKQKVGQVAAEIRAFKKPEPYQ
                     LKGIRYQGEYILKKEGKKK"
     gene            complement(3083..3481)
                     /gene="rpsH"
                     /locus_tag="PK264_20855"
     CDS             complement(3083..3481)
                     /gene="rpsH"
                     /locus_tag="PK264_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRD78354.1"
                     /translation="MSMNDPLGDMLTRIRNAQMRRRPKVECQASMLRVRVLDVLIEEG
                     YIRGYSRVDRKGEMPMLDIELKYNNGAPAIREIKRVSTPGRRVYSPVSDLRAVANGLG
                     VAILSTPKGVMSDQRAREANVGGEILCNVF"
     gene            complement(3495..3800)
                     /gene="rpsN"
                     /locus_tag="PK264_20860"
     CDS             complement(3495..3800)
                     /gene="rpsN"
                     /locus_tag="PK264_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRD78355.1"
                     /translation="MAKISSVEKNNRRRKLSKQFAAKRQRLKDVANDKSKPMEVRFAA
                     RLKLAEMPRNSSATRIRNRCELTGRARGYYRKLKLCRNQLRELASQGLIPGMVKSSW"
     gene            complement(3847..4542)
                     /gene="rplE"
                     /locus_tag="PK264_20865"
     CDS             complement(3847..4542)
                     /gene="rplE"
                     /locus_tag="PK264_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRD78356.1"
                     /translation="MADKEKPEGEKPARPQKEGGKGKPEQGKGKGKGPAKAEAAEKPA
                     PKPRPKDYKPRMKKHYEDVVRAALTEKFSYKNSMQVPRLEKIVLNMGVGEAVADRKKV
                     DNAAGDLALIAGQRPVITRARKAIATDKVRENMPIGAKVTLRGVRMYEFLDRLVTIAL
                     PRVKDFRGLNPASFDGRGNFSMGLKEHIVFPEIDYDKVDQVWGMDVIVCTSARSDDEA
                     RELLKSMYFPFRS"
     gene            complement(4546..4872)
                     /gene="rplX"
                     /locus_tag="PK264_20870"
     CDS             complement(4546..4872)
                     /gene="rplX"
                     /locus_tag="PK264_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRD78357.1"
                     /translation="MASLKIKKGDHVIVLTGRDKGKHGEVLEVIPRENRALVRGVNIV
                     RRHTKQSARSEGGIISKEAPIDLSNLALEDPKDGKPTRVGFKFLDDGRKVRFAKRSGE
                     IIPETK"
     gene            complement(4872..5240)
                     /gene="rplN"
                     /locus_tag="PK264_20875"
     CDS             complement(4872..5240)
                     /gene="rplN"
                     /locus_tag="PK264_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRD78358.1"
                     /translation="MIQMESNLDVADNSGARRVQCIKVLGGSKRKYATVGDTIVVTVK
                     EAIPRGRVKKGQVMKAVIVRTAKGIRRPDGSLIRFDRNAAVLINAQGEPVGTRIFGPV
                     TRELRGKNHMKIVSLAPEVL"
     gene            complement(5310..5546)
                     /gene="rpsQ"
                     /locus_tag="PK264_20880"
     CDS             complement(5310..5546)
                     /gene="rpsQ"
                     /locus_tag="PK264_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRD78359.1"
                     /translation="MPKRILQGVVVSDKNDKTIVVEVERRFTHPLFKKTVRRSKKYHA
                     HDETNTFKVGDVVRIEESAPISKNKRWVVLKTPA"
     gene            complement(5559..5768)
                     /gene="rpmC"
                     /locus_tag="PK264_20885"
     CDS             complement(5559..5768)
                     /gene="rpmC"
                     /locus_tag="PK264_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRD78360.1"
                     /translation="MKASDLKTMTLDQLDDQLLALKKEQFNLRFQAVSGQLENTSRVR
                     QVRRDIARIMTIARQKRGPAPAAGA"
     gene            complement(5818..>6015)
                     /gene="rplP"
                     /locus_tag="PK264_20890"
     CDS             complement(5818..>6015)
                     /gene="rplP"
                     /locus_tag="PK264_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005973000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRD78361.1"
                     /translation="VPVTKKPAEVRMGSGKGSPELWVARVKPGRIIFEIDGVADDIAR
                     EALLLGAAKLPIKTRIVTRLT"
     assembly_gap    6016..6025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6026..6218)
                     /locus_tag="PK264_20895"
                     /pseudo
     CDS             complement(6026..6218)
                     /locus_tag="PK264_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005973000.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L16"
BASE COUNT          923 a         2068 c         1988 g         1267 t
ORIGIN      
        1 cgacgccgcg ctgcgtgatc tgctcgccga gccacatcag gaacatggtg ccgccgacca
       61 gcgtgatcac ggtcgacatg cggaagaaga aaccgggctc gagcacgacg ttgccggcct
      121 ggccgccggt ctgcccctcg aggccgagcg agatgccgta ggcctggaac gtcgcgagaa
      181 tgacggtgag gtagcgcgta tactggttga tctgcttgcg cccctgctcg ccttccttct
      241 tgagggcctc gagtcgaggc gagacgctcg acatcagctg catgatgatc gaggcggaga
      301 tgtagggcat gatgttgagc gcgaagatcg ccatgcgctc cacggcacca cccgcgaaca
      361 tgttgagcat gccgaggatg ttgttcgcgt tgttctggaa ctgctgcgcg aaggctgccg
      421 gattgacgcc cgggatcggg atgtaggtgc cgagccggta gacgagcagg gcaagcacgg
      481 tgaagaggag gcgccgcttc agctccgtcg ccttcgaaaa ggcaccgaag ttgaggttgg
      541 aggccaattg ctctgcggcg gagaccatgt cgcgctatcc cgagatcatt tcaggcaagc
      601 gccacgattg gcgcctgcct gcggttttgt gtccgaacgt gcgttccagt caaccggcgt
      661 cccagtcaat gccgcagtcg caatcgcgcc gcaacggcgc gaggcctcag tcctcggcag
      721 cgccgccgcc cttcttgacc tgcttcgcct tcttggcagc ggacttggcg cgcttggcct
      781 cgtcggcggg gagtaccttg cgctcgatca ccgtgacgga gccgccggcc ttctcgaccg
      841 cggccttggc cgaggccgtc gcatgatcga cggtgatcga gagcttcgcc ttgagctcgc
      901 cgtcgccgag gaggcgaaga ccgtcgtggg cacggcgcac aagccctgct gccaccagtg
      961 ccgcattgtc gaccggcttt gtggcgtcga ggcggccttc gtcgatcgct ttctgcaggg
     1021 cgccgacatt catctcgttg aagcgacggc ggtccatagt gttgaagccg cgcttcggca
     1081 gccggcggaa gagcggcatc tggccgcctt cgaagccgcc gaggcggaca ccggtacgtg
     1141 ccgtctggcc cttgccgccg cggccgccca tcttgcccat gcccgagccg atgccgcgac
     1201 cgatccggac gcgctctttg cgcgagcccg ggttgtcctt cataccattg agccgcatgg
     1261 gcttgctcct cgagccggtg accctctggg tcaggccttt ttgtcatcca cgacccgcac
     1321 caggtgcggg aggctgtcga tcatgccgcg cacagccgga gtgtcctcca gcgtcgagcg
     1381 gcgacggatc ttgttgagct tcaggccgat aagcgtctga cgctggacct cgggccgccg
     1441 gttggggctc gcgtactgct cgatcgtgac ggtcttgccg cccggcttct tggtcgtcat
     1501 gtcacgtcac ctttcgggag ttcaggcggc gtccgggccg ccggcctcgg tctgggcagc
     1561 ctcgccctgg ccggcctggt cacggcggcg ggagatgatc tccgagacct tcttgccacg
     1621 gcgggccgcg acggcacgcg gattttcctg gtgctttagc gcatcgaacg tcgcgcgcac
     1681 catgttgtac gcattcgtcg agccggtgct ctttgagacg acgtcctgca cgccgagcat
     1741 ctccaggacc gcgcgaacgc gaccgccagc gatgatgccc gtgcccgggg gcgccgagcg
     1801 cagcgtcacc ttgccggcgc cgtgatggcc aacgctgtcg tgatgcaggg tccgcttgtc
     1861 gcggagcggc acgcggatga gggcgcggcg cgcgccctcg gtcgccttgc ggatcgcctc
     1921 cggcacctcg cgcgccttgc cgtgaccgaa gccgacgcgg ccctgcttgt cgccgacgac
     1981 gacgagtgcc gcgaagctga agcgcttgcc gcccttgacg accttcgtca cgcggttgat
     2041 gtgcacgagc ttctcggcga actcgttgtc gcggtcgtcg cgatcgcgat cacggtctct
     2101 ggatggattg cgtgccaagg acttgatcct ttcctcagaa gctcaagccg ccggcacggg
     2161 cggcatccgc cagcgccttg acccggccgt ggaatttgta ggcgccacga tcaaacgtga
     2221 cctccttgac gccggccttg atcgcccgct cggcgatcat cttgccgacc tcggtcgccg
     2281 cggacttgtt gccgccggac ttgatccggc cgcgcacgtc cttctcgatg gtcgaggccg
     2341 acgcgagcgt cagccccttc gcatcgtcga tgacctgggc atagatgtgc agatgtgaac
     2401 ggtgcacgga gagccggggc cggccagccg ccaccttctt gagcgtgcgg cggacgcggg
     2461 cccgccggcg ctggaattgg gaatcgtggg cgctcatcgc ggtctctcga ggttacttct
     2521 tcttgccttc cttcttcagg atgtactcac cctgatagcg gatgcccttg agctggtaag
     2581 gctcgggctt cttgaaggcg cggatttcgg cggccacctg gccgaccttc tgtttatcga
     2641 tcccggtgat ctggatctgc tcctgtttgg cgcctgcaat cttgacctcg acgccagcag
     2701 gaatcttgta ggtgatatcg gaggcgaagc cgacctggag gttcaggtcc ttgcccttca
     2761 tctgcgcgcg atagccgacg ccgtggatct cgagcgtgcg cgagtagccg ttctgcacgc
     2821 ccacgatgtt gttggcgatc agcgtgcgag acatgcccca catggagcgc gccgtcttag
     2881 aatcctcgcg cggcgtgagc gcaattccgg ccgccgtctt ctcgaccttc agctcctccg
     2941 gcatgtcgag agcgagctcg cccttcgggc ccttcaacgt aaccttctgt ccggcgaccg
     3001 tcgcttggac gttggctggc acaggtaccg ggcgtttgcc gattcgggac atgtcgtcat
     3061 tcctttcagg gcgcaggcgc gctcagaaca cgttgcagag gatctcgccg ccaacgttgg
     3121 cctcgcgtgc gcgctgatcc gacatcacgc ccttcggcgt cgacaggatc gccacaccga
     3181 ggccgttcgc gacagccctg agatcgctta ccggcgagta cacgcggcgg cctggcgtcg
     3241 acacgcgctt gatctcgcgg atggccggcg cgccgttgtt gtacttgagc tcgatgtcga
     3301 gcatcggcat ctcgcccttg cgatcgacgc gcgagtagcc gcggatatag ccttcctcta
     3361 tgagcacgtc gaggacacgc acgcgcagca tcgatgcctg gcactcgacc ttcgggcggc
     3421 ggcgcatctg cgcattgcgg atgcgcgtca gcatatcgcc aagcggatcg ttcatagaca
     3481 ttgcgacctc gcccttacca gctcgacttg accatgccgg ggatcagccc ctggctcgcc
     3541 agctcgcgca actggttgcg gcagagcttc agcttgcgat agtagccgcg cgctcggccg
     3601 gtgagctcgc agcgattgcg gatccgcgtc gctgacgagt ttctcggcat ctcggcaagc
     3661 ttcaggcggg cggcgaagcg cacctccatc ggcttcgact tgtcgttcgc aacgtccttc
     3721 aggcgctggc gcttggctgc aaactgcttc gaaagcttgc gccggcgatt gttcttctcg
     3781 actgagctga tcttggccat ctgacctcct ggaacccact tttcgtatcg acgacgaagt
     3841 taggtcctag cttcggaacg ggaaatacat gctcttgagg agctcgcgcg cctcatcatc
     3901 cgatcgcgcg cttgtgcaca cgatcacgtc catgccccac acctggtcga ccttgtcgta
     3961 gtcgatctcg gggaacacga tgtgctcctt gaggcccatc gagaagttgc cgcggccgtc
     4021 gaagcttgca ggattgagcc cgcggaagtc cttcacgcgc ggcagcgcga tggtgacgag
     4081 gcgatccaga aactcataca tgcgaacgcc ccgcagcgtc accttggcgc cgatcggcat
     4141 gttctcgcgc accttatcgg tggcgatcgc cttgcgagcg cgtgtgatca cgggccgctg
     4201 gccggcaatc agcgcgaggt cgcctgccgc gttgtcgacc ttcttgcggt cggccacggc
     4261 ctcgcccacg cccatgttga gcacgatctt ctcgagtcgg ggaacctgca tcgagttctt
     4321 gtaggagaac ttctcggtca gggccgcgcg caccacatcc tcgtagtgct tcttcatgcg
     4381 cggcttgtag tccttgggac ggggcttagg cgccggcttc tcggcggcct cggccttggc
     4441 cggacctttg cctttgccct taccctgctc cggcttgccc ttgccgccct ccttctgggg
     4501 acgggccggc ttctcgccct cgggcttctc tttgtcggcc atggcttact tcgtctccgg
     4561 aatgatctcg cccgagcgct tggcgaaacg gaccttgcgg ccgtcatcga ggaacttgaa
     4621 gccgacgcgc gtgggcttgc cgtccttcgg gtcctcgagc gccagattcg agaggtcgat
     4681 cggcgcctct ttcgagatga tgccaccttc ggagcgcgcc gactgcttgg tgtgacggcg
     4741 cacgatattc acgccacgga ccagtgcgcg gttctcgcgc gggatcacct cgagcacctc
     4801 accgtgcttg cccttgtcgc ggcccgtcag cacgatgacg tggtcgccct tcttgatctt
     4861 gagggacgcc attacagcac ctccggcgcg aggctgacga tcttcatgtg gttctttccg
     4921 cgaagctcgc gcgtgacggg gccgaagatg cgggtgccga ccggctcgcc ctgcgcattg
     4981 atcagcacgg ctgcgtttcg atcgaagcgg atcagcgacc cgtccgggcg gcggatgcct
     5041 ttcgccgtgc gcacgatcac ggccttcatc acctggccct tcttgacgcg accgcgcggg
     5101 atcgcctcct tgaccgtcac gacgatcgtg tcgccgacgg ttgcatattt gcgcttggag
     5161 ccgccgagca ccttgatgca ctgaacccgc cgtgcgccgg aattgtcggc gacgtcgagg
     5221 ttgctctcca tctggatcat ggctcgatcc tttcaccctt cacgccgccg cagcggcaca
     5281 tctcgttgga gcggccgagg ccgcccgcct caagccggcg tcttcagcac aacccagcgc
     5341 ttgttcttgg agatcggcgc gctctcctcg atgcgcacca cgtcgccgac cttgaacgtg
     5401 ttggtctcgt cgtgggcgtg atacttcttc gagcggcgaa cggtcttctt gaacagcggg
     5461 tgggtaaagc gacgctcgac ctcgacgacg atcgtcttgt cgttcttgtc ggacaccacg
     5521 acgccctgca ggatgcgctt cggcattgat cgaactcctt aagcgccggc cgcaggcgcg
     5581 gggccgcgct tctgccgggc aatggtcatg atccgcgcga tgtcccgccg gacctggcgg
     5641 acgcgcgatg tgttctcgag ctggcccgac accgcctgaa agcgcaggtt gaactgctcc
     5701 ttcttcaacg cgagcagctg gtcgtcgagc tgatcgagcg tcatcgtctt cagatcactt
     5761 gccttcatcg aacatcgtcc tttgtcgtgc ggccgcgccg ggcgtccagc gaaatcgtca
     5821 ggtgagccgc gtcacgatgc gcgtcttgat cggcagcttt gccgcgccga gcagcaatgc
     5881 ctcgcgggcg atgtcgtcgg caacgccgtc gatctcgaag atgatgcgac ccggcttcac
     5941 gcgcgcgacc cagagctcgg gcgagccctt gccagagccc atgcggacct cggcgggttt
     6001 cttcgttacc ggcacnnnnn nnnnnagtag cggcccgcgc gcttcatgtg gcgcacgatt
     6061 gcccggcgca tcgcctcgat ctggcgcgca gtgagacgct ccggctcgac ggccttgagg
     6121 ccgtgcgatc cgaagttcag cgtcgtaccg cccttcgcag tgccgtgaat gcggcccttg
     6181 aaggccttgc ggaacttggt gcgtttcggt tgcagcatcg tcttcgtccc tggtccggcc
     6241 gtagcaagac cggcct
//
LOCUS       DAORNH010000280         6247 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9297_length_6247_cov_2.702681, whole genome shotgun sequence.
ACCESSION   DAORNH010000280 DAORNH010000000
VERSION     DAORNH010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9297_length_6247_cov_2.702681"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..810)
                     /locus_tag="PK264_20900"
     CDS             complement(130..810)
                     /locus_tag="PK264_20900"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRD78362.1"
                     /translation="MSGLRVIFDLDDTLYPERDFAVAGFQAAGAWAEQALGITGLGDD
                     MTRLLDEGHLGQLFAMALAAKKPDHTADDLAGLRKAYRHNQPKLALYPDAEMALDHFG
                     ALGPIGLITDGTLAMQESKVAGLGIAPRFAEIVYTDALGPDRAFFKPHPRAFEVIAGR
                     MGRPGDRFVYVGDNLAKDFVAPNALGWTTVWIERPLRIHTSATVKPGGEPQHRITSLE
                     DLARLALR"
     gene            complement(807..1763)
                     /gene="prpB"
                     /locus_tag="PK264_20905"
     CDS             complement(807..1763)
                     /gene="prpB"
                     /locus_tag="PK264_20905"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HRD78363.1"
                     /translation="MSTWLERGGLERGGLEAAGTGLERAGDHLRAFMGRQGIVRAPGA
                     HNAMAGLLAKEAGFKALYVSGAAVTASMGLPDLGILTIEELAGVVRSIARATDLPLIV
                     DGDTGYGEALNAMRLVRELEMAGAAAVHIEDQVLPKKCGHLNDKSLVPPDQMAAKIAA
                     ARKAARHLVVIARTDAAASEGVAGAIRRAKLYKSAGAEVLFADALTSLDEFKAFVEAV
                     DAPVLANMTEFGRTPLMTAEALERIGVKIVIWPVSSLRIAAFAMEGFYRDLARDGTTA
                     PQLDRMQTRKRLYEVIRYHEYEALDASIVKSVLPTLPKGSSS"
     gene            complement(1797..>2112)
                     /locus_tag="PK264_20910"
     CDS             complement(1797..>2112)
                     /locus_tag="PK264_20910"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRD78364.1"
                     /translation="GQSYIQRLGCEFDDYFEAEHGHWPVDLNVTYASEREDWVKGLLL
                     AGLGCAIVPEFMEMPRGIVKRPLVDPEVIRTVALATLRGKMLGNAAMAFARVARVHNW
                     AP"
     assembly_gap    2113..2122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2123..2667)
                     /locus_tag="PK264_20915"
     CDS             complement(<2123..2667)
                     /locus_tag="PK264_20915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD78365.1"
                     /translation="METHEIRYFLAMVRELNFTRAAASCNVSQPALTRAIQKLEAELG
                     GALFLRRPGQIELTRLARELLPQLEAIEHGLFAVRNQASQIIEAQASTLRLGVMCTVG
                     PAHLVAMLARVQEVVTDLEVSIVDATAGRIVELLIADQIDVGITAWPAYPPAIGVSPL
                     LKERYVIAMREDDILASESAVP"
     gene            complement(2851..4233)
                     /locus_tag="PK264_20920"
     CDS             complement(2851..4233)
                     /locus_tag="PK264_20920"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRD78366.1"
                     /translation="MRTIGLAIACFMMVHAPSHAQEPKRGAAAPLPAKSARVVPDPAS
                     GVSYAPMLERVIPTVVTVRVTSETLAPVDLTPGATVVTPRRIRARSGGSGVIVDAANG
                     HILTNHHVIADAVAIEVVLSDGRLLPARLVGRDIGSDVAVLEVQERRLPQIPIGDSDR
                     VRVGDVVAAVGNPFGLEGTATLGIVSATMRTEIGHEAFEDYFQIDAVINPGNSGGALI
                     NARGELVGINTATAGTVGNVGIGFAIPINMARVIKTEIIRTGRMRRGSPGLVVEDLSY
                     ERAAELMSGTNRGAIVTAVVPGSPAAAAGISPGAIVIEVAGKPVRGAAEFTTRVVTVP
                     LGTMLSIVVRQNGADKRYELATVDTRLPAREFTFPVEMGAIAGLVASEIALGNPLYGD
                     VRGAFVAGVAPDSPAARSGLAVGDVIVGVDDNNVRHLAHLVRWIDRAGMAYRLRIVRN
                     GVPAWLRMSR"
     gene            complement(4357..4956)
                     /locus_tag="PK264_20925"
     CDS             complement(4357..4956)
                     /locus_tag="PK264_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78367.1"
                     /translation="MRKKLLNLAAVATLGLLAATTSIVAQQAAPFDGKKFFDELSARA
                     VSVPAGFDSKKFFDDIASRGASTKKMFNAQEFFAELAARGVSMKAGFDGNKFFEELAA
                     RGVSMPPMTTMPFDGAKFFAEISARAVNVPSGFDSKKFFDDLASRSVNDKTVFNPNDV
                     FTELSTRGVKMKAGFDPYKFFEEVASRGVGVPPMVAMSK"
     gene            5405..>5840
                     /locus_tag="PK264_20930"
     CDS             5405..>5840
                     /locus_tag="PK264_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78368.1"
                     /translation="MVIIVANESYAAPVPKVEYALRDAEAMQKAAREVLAVPPERIIV
                     LRNLTHAGLAAWFGTAGEGGPELERLKSRLTRTSTITFFYSGHGMPARRSQASEAEAF
                     LLPVDTTPALAARFGIAVSDIRKALLAAQRARSPEGQVVIYLD"
     assembly_gap    5841..6041
                     /estimated_length=201
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6042..>6247
                     /locus_tag="PK264_20935"
     CDS             <6042..>6247
                     /locus_tag="PK264_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78369.1"
                     /translation="ADANRDGAVSARELTDFVTRRIDQRLTHLFPAERRHQTPTLLGD
                     AASRLAMITPATPKRDQDAEARLLA"
BASE COUNT         1027 a         2052 c         1934 g         1023 t
ORIGIN      
        1 agccgcatga tcgccaccgg tccacgacaa gactgcgccg cacgattcct acccgcctgc
       61 cgcgcatctg cctaggtcgc tccgggttgc tgctgagtaa cagcacatga aaaggccatt
      121 cgccttcgct cacctgagtg caagtcttgc caagtcttca agcgacgtga tccggtgctg
      181 cggctcacca cccggtttca cggtcgcgct cgtgtggatg cgcaagggcc gctcgatcca
      241 caccgtcgtc cagccgagcg cattcggtgc gacgaagtcc ttcgcgagat tgtcgccgac
      301 atagacgaag cggtcgccgg gccggcccat gcgcccggcg atcacctcga aggcgcgcgg
      361 atgcggcttg aagaaggcgc gatcgggccc gagcgcatcc gtgtagacga tctcggcaaa
      421 gcggggcgcg atgccgagtc ccgcgacctt gctctcttgc atcgcgagcg taccgtccgt
      481 gatgagcccg atcggtccga gagcgccgaa atggtcgagc gccatctcgg cgtccgggta
      541 gagcgcgagc ttcggctggt tgtggcggta cgctttgcgt aatccagcga ggtcgtcggc
      601 ggtgtggtcg ggtttcttcg cggcgagtgc catggcgaag agttggccaa ggtggccttc
      661 atcgagaaga cgcgtcatgt cgtcgccgag acccgtgatg ccgagcgcct gctccgccca
      721 ggcacccgcc gcctgaaatc cggcaaccgc gaaatcgcgc tcggggtaga gcgtatcgtc
      781 gagatcgaag atgacgcgca gcccgctcac gacgacgagc ccttcggcag cgtcggcagc
      841 acgctcttca cgatcgaggc gtcgagcgcc tcgtactcgt ggtagcggat cacttcgtag
      901 aggcgcttgc gcgtttgcat gcgatcgagt tgcggcgcgg tcgtgccgtc gcgcgcgaga
      961 tcgcggtaga agccttccat cgcgaaggcc gcgatgcgga gcgaggagac aggccagatg
     1021 acgatcttca cgccgatccg ctcgagcgcc tcggcggtca tcagtggtgt gcggccgaac
     1081 tcggtcatgt tggcgaggac cggggcatcg actgcctcca caaacgcttt gaactcgtcg
     1141 agcgaggtca acgcgtcggc gaacagcacc tcggcaccgg cggatttgta gagcttcgcg
     1201 cgccggatcg cgccggcaac gccttccgag gccgcggcgt ccgtgcgcgc gattacgacg
     1261 aggtgacgag cagccttgcg cgcggcagcg atcttcgccg ccatctgatc gggcggaacg
     1321 agcgacttgt cgttcaagtg gccgcacttc ttcggcagca cctgatcctc gatatgaacg
     1381 gccgccgcac ccgccatctc aagctcgcgg acgaggcgca tggcgttgag cgcctcgcca
     1441 taccccgtat cgccgtcgac gatcaggggc aggtctgttg cccgtgcgat cgagcggacg
     1501 acgccagcga gttcctcgat cgtcaggatg ccgaggtcgg gcagccccat gctggccgtc
     1561 accgccgcgc ccgagacata gagcgccttg aatcccgcct ccttggcaag gagcccggcc
     1621 atcgcgttgt gcgcgccggg cgcccgtacg atcccttggc gccccatgaa cgctcgcagg
     1681 tgatcccccg cccgctcgag cccggtcccc gccgcttcga gcccacctct ttcgagccca
     1741 cctctttcga gccaagtcga cattcgtgtg ctcccgcttc gccctgcgca cgctgcctac
     1801 ggcgcccagt tgtgtacccg tgcgacacgg gcaaacgcca tggcggcatt accgagcatc
     1861 ttgccgcgca acgtagccag ggccaccgtg cgaatgactt ccggatcgac caggggtcgt
     1921 ttgacgatgc ctctcggcat ctccatgaac tccgggacga tcgcacagcc gaggcccgca
     1981 agcagcaggc ccttgaccca gtcctcccgt tcgctcgcgt atgtcacgtt gaggtccacc
     2041 ggccaatgcc cgtgctcggc ctcgaagtag tcgtcgaact cgcagccgag gcgctgaata
     2101 tagctctgcc ccnnnnnnnn nnggcacggc gctttcgctg gcgagaatgt catcctctcg
     2161 catcgcaatc acatatcgct ccttcaacag cggactgacg ccgattgccg gcggataagc
     2221 gggccaggcg gtgatgccga catcgatctg gtcggcgatc agcagctcga cgatacggcc
     2281 ggcggtcgca tcgacgatcg agacctcgag gtccgtcacc acctcctgca cccgggccag
     2341 catggcaacg aggtgggcag gccccacggt gcacatgaca ccgaggcgaa gagtgctcgc
     2401 ttgggcctcg atgatctggg aggcctgatt tcgcaccgca aacaatccgt gctcgatcgc
     2461 ctcgagctgc ggcaggagct cgcgtgcgag ccgcgtcagc tcgatctggc ccggccgcct
     2521 gaggaacagg gcgcccccga gttcggcctc gagcttctga attgcgcgtg tgagcgccgg
     2581 ttgcgagaca ttgcacgagg ccgccgcgcg cgtgaaattc agctcgcgca ccatcgcaag
     2641 aaagtagcgg atctcatgcg tctccatgga acctcttcgg cgagttcctg ctcctggcac
     2701 caggctcggg tcggccgacg tccgcagacc cgatgccggc agtactgcgt cttgtgagct
     2761 cacactgcag tctcctgaaa tgcgctccaa tctgcaactt gattgtccag gtctcgtctc
     2821 cgttccatcc gcacgacggc cccgcattga tcaccgcgac atgcggagcc aggccggcac
     2881 cccattgcgc acgatgcgca ggcggtaggc catcccggcg cgatcgatcc aacggacgag
     2941 atgggcgagg tggcggacat tgttgtcgtc gacaccgaca atgacgtcgc cgacggcgag
     3001 gcccgaccgc gccgcagggc tgtccggcgc tacgcctgca acgaaagcgc cccgaacgtc
     3061 gccgtagagt ggatttccaa gcgcgatttc gctcgcgacg aggccggcga tcgcacccat
     3121 ctcaaccgga aaggtgaact cccgcgccgg aaggcgcgtg tcgacggtcg cgagctcgta
     3181 gcgcttgtcc gcaccattct gacgcacgac gatcgacagc atagtgccga gcgggaccgt
     3241 caccacgcgc gtcgtgaatt ccgcggcgcc gcgcactggc ttgccggcca cctcaatcac
     3301 gatcgccccc ggactgatgc ctgcggccgc cgcaggtgag cccggtacca ctgccgtgac
     3361 gatcgcgccg cgattggtgc ccgacatcaa ctcggccgcc cgctcgtacg agagatcctc
     3421 gacgacgagg ccgggcgatc cgcgccgcat ccgtccggta cgaatgatct cggtcttgat
     3481 cacacgtgcc atgttgatcg gaatggcaaa gccgatacca acgttgccga cggtgcccgc
     3541 ggtcgcggtg ttgatgccga cgagctcgcc gcgggcgtta atgagcgcgc cgccagagtt
     3601 gccgggattg atgaccgcat cgatctggaa gtagtcctcg aaggcctcgt gccctatctc
     3661 ggtacgcatg gtcgcgctga cgatgccgag cgtcgccgtg ccttcaagcc cgaacgggtt
     3721 gccgacggcg gccaccacgt cgccgacacg aacccggtcc gaatcgccga tggggatctg
     3781 gggaaggcgc cgctcctgga cctcgagcac ggcgacgtcg ctgccgatat cgcggccgac
     3841 cagccgcgcg ggcaggagcc gcccgtccga gagcacgacc tcgatcgcaa ccgcatcggc
     3901 gatcacgtga tggttggtca ggatgtggcc attggcggcg tcgacgatga cgccggagcc
     3961 gcccgagcgg gccctgatgc gacgcggcgt gacgaccgtt gcgcctggcg tgaggtcgac
     4021 tggtgcaagc gtctcgctcg tcacgcgaac cgtcactacg gttggaatga cgcgctccag
     4081 catcggcgca taggacaccc cactcgcagg gtccggcacg acgcgcgctg acttggcagg
     4141 cagcggcgca gccgcgcccc gcttcggctc ctgcgcatgg ctgggcgcat gaaccatcat
     4201 gaaacacgct atagcgagcc cgatcgtgcg catgtcggaa cacctttggt gagcgttgag
     4261 agttgatcgt gcacccgcga tgggaccggg agtgcagtcc ccgatcccat cggcagggcc
     4321 tgagctgcat ggcgcctggt ggcgtggcgg cagtgcctac ttgctcatcg ccaccatcgg
     4381 cggcacaccc acgccgcggg aggccacctc ctcgaagaac ttgtagggat cgaagcccgc
     4441 cttcatcttg acgccgcgcg tgctgagctc ggtgaagacg tcgttcgggt tgaacacggt
     4501 cttgtcgttc acggagcggc tcgcgagatc gtcgaaaaac ttcttggagt cgaacccgga
     4561 cggcacgttc actgcgcgcg cggaaatctc ggcgaagaac ttggcgccgt cgaagggcat
     4621 ggtcgtcatc ggcggcatgc tgacgccgcg agcggcaagc tcctcgaaga acttgttgcc
     4681 gtcgaagcct gccttcatgc tcacgccgcg ggcggcgagt tccgcaaaga actcctgcgc
     4741 gttgaacatc ttcttggtgc tggcgccgcg gctcgcaatg tcatcgaaga acttcttcga
     4801 gtcgaagccg gccggcacgc tcacggcccg agcggaaagt tcgtcgaaga acttcttgcc
     4861 gtcgaagggt gctgcctgct gcgcaacgat cgacgtcgtc gcagcgagaa gccccaatgt
     4921 tgccacggcg gccagattga ggagtttctt tctcatcgat ggtctccatt cgagttgaac
     4981 acgtcgaggc gccgggggag cgctttgaat gcggcggcgc ctcctcagaa ctaggagcga
     5041 agcgccacca gctgaaatgc cgtttggtca tgatttcgat acgcgcgcgg catgggcggc
     5101 gccggctggc tgacgcagca aggatggcca ttcactcgaa cgccgcgcca ctggcgatgc
     5161 gggccggaga gcaggcgcac cgctcgatgg accggcttat cgatggtcct tgggtgtgaa
     5221 cacgtacgga accagagcat cgcgctcggg acggatatcc gatagggttc cgggtccgtt
     5281 tccacggccg tattcgtgat cgacgttcga aaggcactcg atgctcgccc gcctgctccg
     5341 gatcgccctt gctctcgccg ctctcttggc tgcgccgttg cctgcatcag cgggaccgag
     5401 cgatgtggtc atcatcgtcg caaacgagag ttacgcggcc cccgtcccga aggtcgagta
     5461 tgcgctccgc gatgccgagg ccatgcagaa agccgcacgc gaggtgctgg ctgtgccgcc
     5521 cgagcgcatc atcgtgctgc gcaatctcac acatgcggga ctcgccgcct ggttcggcac
     5581 cgccggcgag ggcggacccg agctcgagcg gctgaagtcg cgcctgacgc gcacctcgac
     5641 gatcaccttc ttctattcgg gccacggcat gccggcacgc cggtcacagg cgagcgaggc
     5701 cgaggcattc ctcctccccg tcgataccac ccccgcgctc gcggcccgat tcggcatcgc
     5761 ggtcagcgac atccgcaagg cgctgctcgc cgcccagcgc gccagatcgc ccgaaggtca
     5821 ggtggtgatc tacctcgatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngccgatgcg aaccgcgatg
     6061 gcgcggtctc cgcgcgcgag ctcaccgact tcgtcacacg ccgcatcgac cagcggttga
     6121 cgcacctgtt tccggccgag cgccgccacc agacgccgac cctcctcggc gatgccgcct
     6181 cccgccttgc catgatcacg cccgcgacgc cgaagcgcga ccaggacgcc gaggcgcgcc
     6241 tcctcgc
//
LOCUS       DAORNH010000281         6235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9326_length_6235_cov_2.665372, whole genome shotgun sequence.
ACCESSION   DAORNH010000281 DAORNH010000000
VERSION     DAORNH010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9326_length_6235_cov_2.665372"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>328)
                     /locus_tag="PK264_20940"
     CDS             complement(<1..>328)
                     /locus_tag="PK264_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-transporting ATPase"
                     /protein_id="HRD78370.1"
                     /translation="APGASVTGGTVNRTGSFVMEARRVGAETLLSRIVAEVASAQRSR
                     APMQDRADTIARYFVPVVVAIAVLAFLVWLALGPTPSLAYAVVAAVSVLIIACPCALG
                     LATPMSI"
     assembly_gap    329..389
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<390..1282)
                     /locus_tag="PK264_20945"
     CDS             complement(<390..1282)
                     /locus_tag="PK264_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HRD78371.1"
                     /translation="MGGLKKIDPVCGMSVEPKPDTPTHEHAGTTYFFCCNGCRTRFAA
                     APARYLDPATKAKAAEAEATSIPAGTIYACPMCPGQEQDGPGICKVCGMALEPIGVPL
                     ADAGPNPELIDFKKRLKVGAALAIPLVLIAMGPHVGLPLHRWIAPRLSQWLELALALP
                     IVAWCGRPFFERALASFRNRSPNMWTLIGLGVGAALLYSIVAVIAPALFPAAARDHRG
                     LVGVYFEAAAVIIVLVLAGQVMELRARARTGEALRALMDLAPKTARRVADGGEDTDVP
                     LASVKAGDRLRVRPGEAVPVDG"
     gene            complement(1449..2580)
                     /gene="prfB"
                     /locus_tag="PK264_20950"
     CDS             complement(join(1449..2510,2512..2580))
                     /gene="prfB"
                     /locus_tag="PK264_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214877.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRD78372.1"
                     /translation="MRAEAQKLIADIDGSLGLLRRSLDWETAEQRLAGLNQQSEDPNL
                     WNDPKRAQKVMRQREMLERAVNGYKSLERGRDDALTLIELGETEKDEATVAEGEAALA
                     KLAIEVQRREVEALLSGEADGLDTYLEVHAREGGTESQDWASILMRMYTRWAERRGFK
                     VSLLDRSDGEEAGIKSVLLEIKGENAFGWLKTEAGTHRLVRISPFDSNARRHTSFASV
                     AVYPVVDDDIEIEINPADVEITTQRSGGAGGQYVNKTESAVRFFHKPSGLNFVVQDQR
                     SQHQNREVGWKRLKARLYEIELKKKEEKAAAEAASKVKVGWGSQIRSYVMQPYQMVKD
                     LRTGVQSSNISGILDGDIDAFIEAALSQRIKGGSGIEVEDIE"
     gene            complement(2696..4885)
                     /locus_tag="PK264_20955"
     CDS             complement(2696..4885)
                     /locus_tag="PK264_20955"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78373.1"
                     /translation="MALPSNLEQYMLELVNEARLNPIANAARYITSYSPLASSDPDIS
                     NAISFFGVNGAALLAAYQALTPTQPLAWNSNIHDASALHNTAMITADAQEHQLPGEPG
                     LGTRLTNAGYTGWNSAGENIYAYSDSVIYGHAGFMVDWGPGPDGMQAGAGHRVNIMSS
                     GFREVGIAIVGESNPATAVGPLVITEDFGRRNSGPAVFILGAAYTDSDANRFYTPGEG
                     LGTLVLSTTSASTTSAASGGYTLGLAAGAQTLTFSGAGLSAPVTATGTFAAGTNAKID
                     IVNGNTLLSSTSVTVNGPVSRIEGLGLTGLSLTGGNGGQTIVGTAGADTLSGLGDGDV
                     LIGGLGGDTLAGGTGFDFAIYASSAAGVTVDLMNSAANAGGDAQGDQLSGIEGLNGSI
                     HADTLRGDDAFNSIYGNSGNDTIEGRGNADALFGNDGTDSLNGGLGGDHIDGGTGFDF
                     AVYAGSAAGVTVDLMNNAANAGGDAAGDQLFFVEGLIGSTHGDTLRGNDDFNSIYGHT
                     GDDTLEGRGGNDALFGNDGTDFLAGGVGGDHLDGGTGYDFVTYAGATSGVRADLLAPA
                     ANTGEAAGDTYNAVDGMIGTSFDDILLGSDGVNAIYGGAGGDTIFGRDGNDSLLGNSG
                     NDVLVGGRGNDTLFGSSTDFAALDNDGFVYEGPGYGQDVIYGFGANAGANHDIISFST
                     SIFSSFSAVLAASQQMGTDTVIFSGADRIQLVHVSVAQLSADDFNFF"
     gene            5303..>6235
                     /locus_tag="PK264_20960"
     CDS             5303..>6235
                     /locus_tag="PK264_20960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRD78374.1"
                     /translation="MPSIDIVLSERICRARNALRLAVIAVTSLLLAPVSAQAEGCRAA
                     DGGLGVSRIVEIDPKSGPLYGHLSRYEKEPAFLAPKEVVLTFDDGPAPGLTTSILETL
                     DRFCTKATFFVVGQMALATPDGVGQIIARGHTLGTHTWSHPMNMPRLTLDRARNQIES
                     GFAAAAIVSKGQVVPFFRFPGLSDSPALLAYLQDRGIATFTVDVVSNDSYIADPQRLI
                     HLTFERLEAQGRGILLFHDIKSVTARALPTILARLKERGYKVVHLKSSRTYEPLPEYV
                     ASVRKSAESARNAKRLLPFFAAAGVGDGSDAPAPL"
BASE COUNT         1005 a         1990 c         2103 g         1076 t
ORIGIN      
        1 gatcgacatc ggcgttgcga ggccgagcgc acacgggcag gcgatgatga gcacgctgac
       61 ggcggcgacg acggcgtagg caagcgacgg cgtcggcccg agcgccagcc agacgaggaa
      121 ggcgaggacc gcgatcgcca ccacgaccgg cacgaagtag cgcgcgatcg tatcggcgcg
      181 gtcctgcata ggcgcgcggc tgcgctgcgc ggaggcgacc tcggcgacga tgcgcgagag
      241 cagggtctcg gcgccgacgc gacgggcctc catcacgaac gagcccgtcc gattgacggt
      301 accgccggtg acggatgcgc ccggcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc cgtcgaccgg cacggcctcg cccggccgca
      421 cgcggagacg gtcgccggct ttcaccgagg cgagcggtac atcggtgtct tcgccgccat
      481 cggcaacacg acgcgctgtc ttaggtgcga ggtccatgag cgcacgtaag gcctcgccgg
      541 tgcgggcgcg agcgcgcagc tccatcacct gcccggcgag cacgagcacg atgatcaccg
      601 ccgcggcctc gaagtagacg ccgacgaggc cgcggtgatc gcgggccgct gccggaaaga
      661 gcgccggcgc gatcaccgcg acgatgctgt agaggagtgc ggcgccgacg ccgaggccga
      721 tcagcgtcca catgttcggg ctgcggttcc tgaacgaggc aagcgcccgc tcgaagaatg
      781 gccgcccgca ccaggcgacg atcggcaggg cgagagcaag ctccagccat tgcgagaggc
      841 gcggggcgat ccagcggtgg agcggcaggc cgacgtgcgg ccccatggcg atcaggacga
      901 gcgggatcgc cagcgccgcg ccgaccttca gtcgcttctt gaaatcgatg agctcggggt
      961 tcgggccggc gtcggcgaga ggcacgccga tcggctcgag cgccatgccg cagaccttgc
     1021 aaatgccggg gccgtcctgc tcctgacctg ggcacatagg gcaagcatag atcgtgccgg
     1081 cgggaatgga cgtggcctcc gcctcggcgg ccttggcctt cgtggccgga tcgaggtagc
     1141 gggcgggggc ggcggcaaag cgcgtgcgac agccgttgca gcagaagaag taggtcgtcc
     1201 cggcgtgctc gtgcgtcggc gtatcgggct tgggctcgac gctcatgccg cagacgggat
     1261 cgatcttttt cagcccgccc atcgcggccg ccggggcgtg cccggtgcca tggccagcgc
     1321 catgtccgca tagtgggccg tgtgactgca tggcgtggct ctctgatcgc cggagttcgg
     1381 tagacgggta cggcttcgag ggaccacaat gcggggcttg tcggcctgat ctcggtcaat
     1441 ccggcccgct attcgatgtc ttccacctcg atgccgctac cgcccttgat gcgctgcgag
     1501 agcgccgcct cgataaaggc atcgatatcg ccgtcgagga tgccggagat gttggagctc
     1561 tgcacgccag tgcgcaggtc cttgaccatc tgatagggct gcatgacgta ggagcggatc
     1621 tggctgcccc agccgacctt gaccttggag gcggcctcgg cggcggcctt ctcctccttc
     1681 tttttcagtt cgatctcata gaggcgcgcc ttcaagcgct tccagccgac ctcgcggttc
     1741 tggtgctgcg agcgctggtc ctggacgacg aaattgaggc ccgagggctt gtggaagaag
     1801 cggacggccg attcggtctt attgacatac tggccgcctg cgccgcccga gcgctgcgtc
     1861 gtgatctcga cgtcggcggg gtttatctcg atctcaatgt cgtcgtcgac gacggggtag
     1921 accgcgacgc tcgcaaagct cgtatggcgc cgcgcgttgg aatcgaacgg cgagatgcgc
     1981 acgaggcggt gcgtgcccgc ttccgtcttc agccagccga aggcgttctc gcccttgatc
     2041 tccaggagca ccgacttgat gccggcctcc tcgccgtcgc tgcggtcgag caggctaacc
     2101 ttgaagccgc gccgctccgc ccagcgtgtg tacatgcgca tgaggatcga ggcccagtcc
     2161 tggctctccg tgccgccttc gcgcgcatgc acctcgaggt aggtatcgag gccgtccgct
     2221 tcgcctgaga gcagcgcctc gacctcgcgc cgctgcactt caatcgcgag cttggcgagt
     2281 gccgcctcgc cctcggcgac tgttgcctcg tccttctcgg tctcgccgag ttcgatgagt
     2341 gtcaatgcat cgtcacggcc gcgctcgagc gacttgtagc cgttgaccgc gcgctccagc
     2401 atctcgcgct ggcgcattac cttctgggcg cgcttcggat cgttccagag attggggtcc
     2461 tcggactgct ggttgagtcc ggcgaggcgc tgctctgccg tctcccagtc aaagtgacct
     2521 ccgtaggagg ccgagcgagc cgtcgatatc ggcaatcagc ttctgggctt cggcgcgcat
     2581 ggggggctcc gcagtgaaaa cgatgtttcg ctgcatatag gcgcggcaag gcccactgta
     2641 aacgtggccg gctgcctcgc gaggcggagc gggaccgctg tggtcggcag ccggctcaga
     2701 agaagttgaa atcgtccgcc gagagctgtg ccacgctgac atgcaccagc tggatgcggt
     2761 cggcgcccga gaagatgacc gtatcggtgc ccatctgctg cgatgccgcg agaactgccg
     2821 aaaagctcga gaagatcgac gtgctgaagc ttatgatgtc gtggttcgcg ccggcattgg
     2881 cgccgaatcc gtagatcacg tcctggccgt agcccgggcc ctcgtagacg aagccgtcgt
     2941 tatcgagggc ggcgaaatcg gtgctcgagc cgaacaacgt gtcgttgccc ctgccgccga
     3001 caagcacgtc gttgccggaa ttgccgagga gcgaatcatt gccgtcgcgg ccgaagatcg
     3061 tgtcgccgcc ggcgccgccg tagatcgcgt tcacgccgtc cgagccgagc agaatgtcgt
     3121 cgaagctcgt gccgatcatg ccgtcgacgg cattgtaggt gtcgcccgcg gcctcgcctg
     3181 tattggcggc cggcgcgagg aggtcggcgc gtactcccga cgtcgcgccc gcgtaggtca
     3241 cgaagtcgta gccggtgccg ccgtcgagat gatcgccgcc gacgccgcct gccaggaagt
     3301 ccgtgccgtc gttgccgaac agcgcatcgt tgccgccgcg gccttcgagc gtatcgtcgc
     3361 cggtgtggcc gtagatcgag ttgaaatcgt cgttgccgcg gagcgtgtcg ccgtgcgtcg
     3421 agccgatcag cccttcgacg aagaagagct ggtcacctgc ggcatcgccg cctgcgttgg
     3481 cagcattgtt catgagatcg acggtcacgc cggccgccga gcccgcatag accgcgaagt
     3541 cgaagccggt gccgccgtca atgtggtcac cgccgaggcc gccgttgagg ctgtcggtgc
     3601 cgtcgttgcc gaagagggca tccgcattgc cgcgcccctc gatcgtgtcg ttgccggagt
     3661 tgccgtagat cgagttgaag gcgtcgtcac cgcgcagcgt atcggcatga atcgagccgt
     3721 tgagcccttc gattccggag agttgatcgc cctgcgcgtc gccaccggca ttggcggcac
     3781 tattcatgag atcgacagtg acacctgcgg ctgacgacgc gtagatcgcg aagtcgaagc
     3841 cggtgccgcc ggcgagcgta tcgccaccga gcccgccgat cagcacgtct ccgtctccga
     3901 gcccggagag ggtgtcggcg ccggccgtcc cgacgatcgt ctgcccgccg ttgccgccgg
     3961 tgagcgagag gcccgtcaaa ccgagccctt cgatgcgcga gaccgggccg ttcacagtca
     4021 cggacgttga ggacagcagc gtgttgccgt tgacgatatc gatcttggcg ttggtcccgg
     4081 cggcgaaggt gccggtggcg gtcacgggtg cgctgaggcc agcgcccgag aacgtgagcg
     4141 tttgcgcgcc ggccgcgagc ccgagcgtat agccgccact cgcagccgac gtggtgctcg
     4201 ccgaggtcgt cgagagcaca agcgtgccga ggccttcgcc cggtgtgtag aagcgatttg
     4261 cgtcgctgtc ggtgtaggcg gcgccgagga tgaacacggc tgggcccgaa ttgcggcgac
     4321 cgaagtcctc ggtgatgacc agcgggccga ccgccgttgc cgggttgctt tccccgacga
     4381 tggcgatgcc gacctcgcgg aaccccgagc tcatgatgtt cacgcgatga ccggcgcccg
     4441 cctgcatgcc gtccggcccc ggaccccaat cgaccatgaa gccggcgtgg ccatagatga
     4501 cgctgtcgga ataggcgtag atgttctcgc cggccgagtt ccagccggta tagcccgcat
     4561 tggtgaggcg cgtgccgagg ccaggctcgc cggggagttg gtgctcctgg gcatccgccg
     4621 tgatcatcgc agtattgtgc agggccgagg cgtcgtggat gttcgaattc caggcgagtg
     4681 gctgcgtggg ggtgagcgcc tggtaagcag caagcagtgc ggccccattc acaccaaaga
     4741 acgatatcgc attggagatg tccggatcac tcgaggcgag cggcgaatac gaagtgatgt
     4801 agcgcgcggc attcgcgatc gggttgaggc gcgcctcgtt gaccagctcg agcatgtact
     4861 gctcgaggtt cgatggcaac gccatgggaa actccgcaca agaagacttg atcctgtcac
     4921 gtcgtaacca taagcctttg atatcaatgc tcccctaata ccggcgacag tgttttactc
     4981 acgcaaagat tgcggcaatc aactttagcg acctgtgatt gaggtgcgtc tttttcgtcg
     5041 cttcggcggg acaagtgtcg ccgccttgaa ctgatggctt gtattcgccg gtgctgaatg
     5101 gataatccga ctgcgcccgc gatttggagt ttcctagcct cgaggtggat cgggcgaacg
     5161 cgagtgcgct gtccgagaca ttggacagcc ggtaacgcga cacgcgggca agcgccgtga
     5221 tgggcgccgg ggcggagcgt gaactgcaag tgatgcggcg tgtgcgtgcg tcccggaacg
     5281 gtccgggtca acgaggtgat tcatgccgtc gatcgacatc gttctgtcgg agcgcatctg
     5341 tcgagcacgc aatgctctgc gcctcgccgt catcgccgtc acgagcctgc tgctcgcgcc
     5401 ggtaagtgcg caggcagaag gttgccgcgc agccgatggc gggcttggcg tctcgcgcat
     5461 cgtcgagatc gacccgaagt ccgggccgct ctacggccac ctctcccgct acgagaagga
     5521 gcccgcgttc ctcgcgccga aggaggtcgt gctgaccttc gacgacggcc cggcgcccgg
     5581 cctcaccacc tcgatcctcg agacactcga tcgattctgc accaaggcga cgtttttcgt
     5641 ggtcggccag atggcgctcg cgacgcccga cggcgtcggc cagatcatcg cgcgcggaca
     5701 tacgctcggc acgcacacct ggtcgcatcc gatgaacatg ccgaggctca cgctcgatcg
     5761 cgcgcgcaat cagatcgaga gcggcttcgc ggccgcggcc atcgtctcga aggggcaggt
     5821 cgtgccgttc ttccgcttcc cggggctgtc ggattcgccg gcgctgctcg cctatctgca
     5881 ggaccgaggc atcgcgacct tcacggtcga cgtggtctcc aacgattcct acatcgccga
     5941 tccgcagcgc ctcatccatt tgaccttcga gcggctcgag gcgcaggggc gcggaattct
     6001 cctcttccac gacatcaagt cggtgacggc ccgcgccctg ccgacgatcc tcgccaggct
     6061 caaggagcgt ggctacaaag tcgtgcatct gaaatcgagc cgcacctatg agccgctgcc
     6121 cgagtacgtc gcaagcgtga ggaaaagcgc tgaaagcgcg cgcaatgcca agcggctgct
     6181 gccgttcttc gcggcggccg gcgtcggcga cggcagcgac gcgccggcgc ctctc
//
LOCUS       DAORNH010000282         6214 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9359_length_6214_cov_2.857120, whole genome shotgun sequence.
ACCESSION   DAORNH010000282 DAORNH010000000
VERSION     DAORNH010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9359_length_6214_cov_2.857120"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="PK264_20965"
     CDS             <1..1789
                     /locus_tag="PK264_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRD78375.1"
                     /translation="CTRARLASGIAPVAQLLRLDLLGVPLARPQSAKATSANGGTGLN
                     HEDEADAMGEAPRGPPPLANGRNVQPPPAPPHLAEPSIAPARPASGGPPPLPPIPATP
                     TLRADLDNPDQLVPLPPSAAFSDWTGLFGAQAAQPAAPPAAPAADEEARPDDLPLSVL
                     LDENSSNASSILAGRELKARKRPITLNRDVLKRHAAVLGGSGSGKTTLALSLIEQLLL
                     RGVPAILIDRKGDLCSYANPEVWRHIDDEWGERRGDREKLADAIDVAVYTPGRSSGRP
                     ISITLLPNGIGELPEHEQQLLANMSAAALGDMLHLKNSAMHQKQSGTLSVALKILGSR
                     YKREVMLADLIHLLEDEDPELTDLTQRMDPSGRVRRDLVAQLDSLRHRNAVLFEGGGE
                     PMRMEAFLGLGSFARLNRTRLSIIYTGFLGDNENILFWVSQFLSEALRFCQRNPNDAL
                     QAVVMLDEADLYIPANAKPATAEPLQSLLKRARSAGLGIMLATQSPGDLDYKSRDQIT
                     SWFIGRVREDTALRKLKAAFQSESGLDPAAVLPHQTVGEFHLVQEGFVRPMKAQRSLI
                     NAEQVPFDRIEQLAYETKGQDERQLKLFK"
     gene            2235..2912
                     /locus_tag="PK264_20970"
     CDS             2235..2912
                     /locus_tag="PK264_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78376.1"
                     /translation="MHKLRVTERRPPVHRPGLALLALLMIASWPSSRAHACACCVNVG
                     ERGERMHALSQFEIDELTGLRAVPRAVLVVTEAFPEDVKGITRPAMEPYIAAGGIDAG
                     RFRVTITGADGRKGTLTLALPKRFLRREIDVPPHPDSDAAKTRTLRGNATLYKEWIFS
                     GDITGDGIFVASTLATAKAELVLHGHGNGCPSGADFHHWTLVVKGRAASFKLHGGLTP
                     PPAPKPK"
     gene            complement(2966..4066)
                     /locus_tag="PK264_20975"
     CDS             complement(2966..4066)
                     /locus_tag="PK264_20975"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HRD78377.1"
                     /translation="MRTRAAVCTGVNKPLELLELEQEGPKAGEARVKVKAAGVCMSDW
                     HIMNGDWPLPLPMVLGHEAAGEVVEVGPGVTNVKKGDHVIFSFRSNCGHCSYCSRGRT
                     VLCIGHNDTSRWVMHDGTSRVKLNGTPMNVMARIGTFTEYSVCPAEQLVPVRKDLPWT
                     HAAIIGCSVATGVGAAVRHAKVEAGSSVLVVGCGGVGLNVIQGAKLAGAKTIIACDLL
                     DNKLQMATQFGATHIINGKRQDVIKTVMEITGLGVDYAFDAIGTEPTVLQIVDALAPA
                     GHAVIVGIPSFATRAPLAPHLMVYGEKTLSGTYYGSVRPNVDFGILADLAMERRINLD
                     SLISRTYKFDEINEGFRLMQGGQVARGVILMD"
     gene            complement(4172..4519)
                     /locus_tag="PK264_20980"
     CDS             complement(4172..4519)
                     /locus_tag="PK264_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78378.1"
                     /translation="MVNMIRVGQANFDTAIKSGFRGLTAALALPILLMALTQWPLADN
                     LRATAGFFSATSRIIYRIADEIEVMTADRSDRAKEKRGRPAEAGSQPDRQNRAGNAAG
                     VLADATTASPVSP"
     gene            4742..5452
                     /locus_tag="PK264_20985"
     CDS             4742..5452
                     /locus_tag="PK264_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78379.1"
                     /translation="MSGNAIDIDKLLTALTPERRELMRPLIGRLNELDDAVVAEALLS
                     LLSREFSAQDEGAKALAEVQRSGGSILAIRETHRELATELASKLLNRASLIAENVKRL
                     NDADAPRAPQATAKPSDSEGVTAPHRQPAPTEASSPTDTRMNSTLRNYWFLVRLEKAN
                     APIARAVLTSEVMQRGFSPNGDNAAATALARLVKDGYLTRPRDGYFSITDLGRKHIAE
                     LKAKIEETGGDVPTVPSA"
     gene            complement(5466..>6214)
                     /locus_tag="PK264_20990"
     CDS             complement(5466..>6214)
                     /locus_tag="PK264_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRD78380.1"
                     /translation="TVTTLADDKVLALYEKGSYEVVAHDNMRRVIAERLTQSKQTVPH
                     FYMSVDCRLDQLLSARERLNASAPKDGPRAFKLSVNDFVIKALALALQQVPAANATWT
                     EGGMLRHRRSDVGVAVAVEGGLFTPVIRHADLKTLAEISNEMKDLAERARKRRLAPHE
                     YQGGTTSISNLGMYGIKSFDAVINPPHATILAVGAGEKRPVVVGDKLEVATLMTATLS
                     CDHRVVDGAIGAELLNAFKAMIEEPVRMLV"
BASE COUNT         1041 a         2152 c         1980 g         1041 t
ORIGIN      
        1 gtgcacgcgg gcgcgcctcg cctcgggaat tgcgccggtc gcgcagctgc tccgtctcga
       61 tctcctcggt gtgccgctgg cgcggcctca gtccgcaaag gccaccagcg caaacggtgg
      121 caccgggctc aatcacgagg atgaggccga tgctatgggt gaggcgccgc gcggtccgcc
      181 gccgcttgcc aatggccgta acgtccagcc gccaccggca ccgcctcact tggccgagcc
      241 atcgatcgcg cctgcccgcc ccgcctcagg cggtccgccg ccgctgccgc cgatccccgc
      301 gacgccgaca ttgcgtgccg atctcgacaa tcccgatcag ctcgtgccgc tgccgccgtc
      361 tgcggccttc tctgactgga caggcctctt cggcgctcag gctgcgcagc cggcagcgcc
      421 gcctgccgcg ccggcagccg acgaggaggc gcggcccgac gacctcccgc tctcggtgct
      481 cctcgacgag aacagctcga acgcctcgag cattctcgcc ggacgcgagc tcaaggcccg
      541 caagcgcccg atcacgctca accgcgatgt cctgaagcgc cacgcggccg tgcttggcgg
      601 ctcgggctcg ggcaagacga cacttgccct ctcgctcatc gagcagcttc tgctgcgcgg
      661 cgtgccggcg atcctgatcg accgcaaggg cgacctctgc tcctacgcca acccggaggt
      721 gtggcggcac atcgacgacg agtggggcga gcggcgcggc gaccgcgaga agctcgccga
      781 cgcgatcgac gtcgccgtgt acactccggg gcgttcctcg ggccgaccga tctcgatcac
      841 gctgcttccg aacggcatcg gcgagcttcc cgagcacgag cagcagctgc ttgccaacat
      901 gtcggcggca gcgctcggcg acatgctgca cctcaagaat tcggcgatgc accagaagca
      961 gtccggcacg ctctcagtcg ccttgaaaat cctcggctcc cgctacaagc gcgaggtgat
     1021 gctcgccgac ctcatccacc tgctggagga cgaggatccc gagctcaccg atctcacaca
     1081 gcgcatggac ccctcgggcc gcgtccgccg cgacctcgtc gcccagctcg acagcctccg
     1141 ccatcgcaac gctgtgctgt tcgagggtgg cggcgagccg atgcgcatgg aagccttcct
     1201 cgggctcggg tcgttcgcga gactgaaccg cacgcggctg tcgatcatct acacgggctt
     1261 cctcggcgac aacgagaaca tcctgttctg ggtctcgcag ttcctctccg aagccttgcg
     1321 cttctgccag aggaacccga atgacgcgct gcaggccgtc gtcatgctcg acgaggccga
     1381 cctctacatc ccggcgaacg cgaagcccgc gacggccgag ccgctgcaga gcctgctcaa
     1441 gcgcgcccgc tccgcaggtc tcggcatcat gctcgccacg cagtcgccgg gcgacctcga
     1501 ctacaagagc cgcgaccaga tcacgagctg gttcatcggc cgcgtgcgcg aggatacggc
     1561 actgcgcaag ctcaaggcgg ccttccagtc ggaatcgggc ctcgacccgg cagccgtgct
     1621 gccgcaccag accgttggcg agttccacct cgtgcaggaa ggcttcgtac gtcccatgaa
     1681 ggcgcagcgc tcgctcatca acgccgagca ggtgcccttc gaccgcatcg agcaactcgc
     1741 ctacgagacg aaaggccagg acgagcggca gctgaaactg ttcaagtgag aggtgcgtgg
     1801 gtcgctcgtg cgcgacggcc tgcctcgatc gtcagggccg cgcgcgccca gtcatctcct
     1861 caccgagcag gcccagtcct gcggactagc gagcccgtcc gacgtcgccc ccggtcccga
     1921 tcacgccttg cacgcgttac cgtcgtcgat cgaccgttgg gtgcctcacg atactcgcag
     1981 ccgccgcccg cggggatagc cgctcgcttt gcgattgggc cacatgacgt aggttggcgc
     2041 tgagcgcagc gaagcccaac aacccaggat gcgaccttgg tgttgggctt cgcagataac
     2101 tcagcccaac ctacagctcc tccgtcgggg ccggcccgcg ccccgacacg ggccgatcct
     2161 gacggcagag gggctactcg acctcgctgg cacgtcatgg caagaccatc gtcggcatga
     2221 ccgaggagac gccgatgcac aagctgcgag tgacggaacg ccgacctccc gtgcaccgtc
     2281 ccggattggc actgctggct cttctcatga tcgcaagctg gccatcttct cgcgcacatg
     2341 cctgtgcctg ctgcgtcaac gtcggtgagc gcggcgagcg catgcacgcc ctctcccagt
     2401 tcgagatcga cgagctcacg gggctccgcg ccgtaccgcg cgccgtcctg gtcgtcacgg
     2461 aagcgttccc cgaggacgta aagggcatca cacgccccgc gatggagccc tatatcgctg
     2521 ccggcggcat cgatgccggg cgcttccgcg tgaccatcac aggcgcggac ggccgcaaag
     2581 gcacgctgac actcgcgctc ccgaagcgct tcctcaggcg cgagatcgat gtccccccgc
     2641 atcccgacag cgacgcggcc aagacgcgga cgctccgcgg caacgctacg ctctacaagg
     2701 agtggatctt ctctggcgac atcaccggcg acggcatctt cgtcgcgtcg acattggcca
     2761 ccgccaaagc cgagctcgtg ctgcacggtc acggaaatgg ctgcccgagc ggcgctgact
     2821 tccaccactg gacattggtc gtcaaaggac gcgcggcatc gttcaagctg cacggcggct
     2881 tgacgccacc gcccgccccc aagccgaagt agtcgcgagg cgaggccgcc tcaactcacg
     2941 ccgacggccg tacccgcagc agagctcaat ccatcaggat cacgccgcgc gctacttggc
     3001 cgccctgcat caggcggaag ccttcgttga tctcgtcgaa cttgtaggtg cgcgagatca
     3061 ggctatcgag gttgatgcgc ctttccatcg cgaggtcggc gaggatgccg aagtcgacat
     3121 tcgggcgcac cgagccgtag taggtgcccg aaagggtctt ctcgccgtag accatgagat
     3181 gcggcgcgag cggcgcccgc gtcgcgaacg agggaatgcc gacgatcacg gcgtggccag
     3241 ccggcgccag cgcatcgacg atctgtagca ccgtcggctc cgtgccgatc gcatcgaagg
     3301 cgtagtcgac gccgagcccg gtgatctcca tcaccgtctt gatcacgtcc tggcgcttcc
     3361 cgttgatgat gtgcgttgcg ccgaactgcg tcgccatctg cagcttgttg tcgaggaggt
     3421 cgcaggcgat gatcgtcttc gcgccggcga gcttggcgcc ctggatcaca ttgaggccga
     3481 cgccgccgca gccgacgacg agcacgctcg agcccgcctc gaccttggca tgccggaccg
     3541 ccgcgccgac gccggtcgca accgagcagc cgatgatcgc cgcatgcgtc caggggagat
     3601 ccttgcgaac gggaacgagt tgctcggccg ggcacaccga atactccgtg aaggtgccga
     3661 tgcgggccat cacgttcatg ggcgtgccgt tgagcttcac gcggctcgtg ccgtcgtgca
     3721 tcacccagcg agaggtatcg ttgtggccga tgcagagcac cgtgcgtccc ctcgagcagt
     3781 acgagcagtg cccgcagttc gagcggaacg agaagatcac gtgatcgccc ttcttcacat
     3841 tcgtgacgcc gggcccgact tcgaccacct cgcccgccgc ctcgtgacca agcaccatcg
     3901 gcaacggtag cggccagtcg ccgttcatga tgtgccagtc ggacatgcag acgcccgcgg
     3961 ccttgacctt gacgcgcgct tcgccggcct tcgggccctc ttgctcgagc tccagcagtt
     4021 cgagcggctt gttcactcca gtgcagacgg cggcgcgcgt gcgcatgtga ggctccccgg
     4081 gttccagtga ctgcgtcggt cgggatcgat aatcctatgg atgccggaac tcggcaatcg
     4141 ccccgcggct tggcagctcg tctccttctg cttacggcga gacggggctc gccgtggtag
     4201 catccgcgag cacgcctgca gcattgccgg cgcggttctg ccgatcgggt tgcgaaccgg
     4261 cctccgcggg gcgcccgcgc ttctcctttg cccggtccga cctgtctgcc gtcatcacct
     4321 cgatctcgtc ggcgatgcga tagatgatgc ggctcgttgc cgaaaaaaag ccggccgtag
     4381 cccggaggtt gtcggcgagg ggccactgcg tcagcgccat gagcaggatc ggcaaggcca
     4441 gagccgccgt cagaccccgg aacccggact tgatggctgt atcgaagttt gcttgcccga
     4501 ctcgaatcat gttaaccatg cgtccatctc cgactcttat actattcgaa gagatcgatt
     4561 cgattgatct ctatgggctg tacgtatcat acgcatagaa cgtacggacg tcaagatgcg
     4621 ctcttggcgt ctttcctgtt ttgattttgt gtgatgggcg atgcggctca gtgctcatct
     4681 cagcaaagta ttgaccacgc cgcgctatac agtatgttcg cttcagtagc gtgagggtgc
     4741 catgagcggt aatgccatcg atatcgacaa gctgctgacg gcattgacgc ccgagcgccg
     4801 ggagctgatg cgccccttga ttggtcggct gaacgagctt gacgacgccg tggttgcgga
     4861 ggctctcctg tcgctactgt cgagggagtt cagtgctcag gacgaaggtg ccaaggcgct
     4921 cgcagaggtg cagaggagcg gcggcagcat tctcgccatc cgcgaaacgc accgcgagct
     4981 cgcgaccgag ctcgccagca agcttctcaa tcgtgcgagc ctgatcgccg agaacgtcaa
     5041 gcggctcaat gacgccgatg cgccccgcgc cccacaggcc acggcgaagc catcggactc
     5101 cgagggcgtg acagctcccc accgccagcc cgctcccacc gaagcatcgt cgccgaccga
     5161 cacgcgcatg aattcgacgc tgcgcaacta ctggttcctg gtgcgcctgg agaaagcgaa
     5221 cgcgccgatt gcgcgcgcgg tgttgaccag cgaggtcatg cagcgcggct tctcgccgaa
     5281 cggcgacaac gcggccgcca cggcactcgc ccgtctcgtc aaggacggct atctgacccg
     5341 tccgagagat ggctacttca gcatcaccga tctgggccgc aagcacatcg cggagctgaa
     5401 ggcgaagatc gaagagaccg gtggcgacgt gccgaccgtt ccctcggcct gatccagccg
     5461 accgctcaca ccagcattct gaccggctcc tcgatcatcg ccttgaaggc gttgaggagc
     5521 tctgcgccta tggcgccgtc gacgacgcgg tggtcgcagg agagggtggc ggtcatgagg
     5581 gtcgcgacct cgagcttgtc gccgacgacg acgggccgct tctcgcccgc gccaacggcg
     5641 aggatggtcg cgtgcggcgg attgatcacg gcgtcaaagc tcttgatgcc gtacatgccg
     5701 aggttggaga tcgaggtggt gccgccctgg tactcgtgcg gggcgagccg gcgcttgcgc
     5761 gcccgctccg cgaggtcctt catctcgttg gagatctcgg cgagcgtctt caggtccgcg
     5821 tgacggatca cgggcgtgaa gaggccgccc tcgacggcga ccgcgacgcc gacatccgag
     5881 cggcggtggc ggagcatgcc gccctcggtc caggtggcgt ttgccgccgg cacctgctgg
     5941 agcgccagtg cgagggcctt gatgacgaaa tcgttgaccg agagcttgaa ggcgcgcggg
     6001 ccgtccttcg gcgcgctcgc gttgagccgc tcgcgcgcgg agagcagctg gtcgaggcgg
     6061 caatcgaccg acatgtagaa gtgcggcacc gtctgcttcg actgcgtgag ccgctcggcg
     6121 atcacgcggc gcatgttgtc gtgcgcgacg acctcgtagc tgcccttctc gtagagggcg
     6181 agcaccttgt cgtccgcgag cgtggtcacc gtac
//
LOCUS       DAORNH010000283         6196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9397_length_6196_cov_2.692070, whole genome shotgun sequence.
ACCESSION   DAORNH010000283 DAORNH010000000
VERSION     DAORNH010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9397_length_6196_cov_2.692070"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..470
                     /locus_tag="PK264_20995"
     CDS             54..470
                     /locus_tag="PK264_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD78381.1"
                     /translation="MATVKMLTDAEASPEARAVFDDIRKTRKSDFVNNAWRAQANHPA
                     MLKRNWEHAKRTMAPGALDALTKEFVYLAVSIMNNCEYCIHSHTFFARQKGMTDAQYS
                     EFLEVIGLATEGNRMMTAMNVPVDERFKVKTTGIDE"
     gene            467..883
                     /locus_tag="PK264_21000"
     CDS             467..883
                     /locus_tag="PK264_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78382.1"
                     /translation="MRRRVLALRAAAASVAALAAASAFAAEPSGGGSLPLADVIERMK
                     RYPNLVQQIKLELVRVGLKREAVVCSGQRFGREWKMLGGERTLPYRCELGRRRLAIDG
                     QRVYLDAAGKRLEASDPKLTERAARVLESRITWVWR"
     gene            880..1353
                     /locus_tag="PK264_21005"
     CDS             880..1353
                     /locus_tag="PK264_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD78383.1"
                     /translation="MSEARTTGRAERPLVRPLAARDKDAWRALFDAYIAFYRAEVADA
                     VIEATWERMLALSHGMTGLVAVDGGDRPIGLAHLVFHVSTWSPTTYCYLEDLYVAPEH
                     QGRGAGHALVEATYREAEARAAKRVYWVTEEDNQAARRLYDTLGRRMHYVQYRRE"
     gene            1527..1838
                     /locus_tag="PK264_21010"
     CDS             1527..1838
                     /locus_tag="PK264_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78384.1"
                     /translation="MNATTLGEIRASAAARDLTDDKMQQVRELLYGEFKRESDARVAL
                     LENRVRELESGLHRRLDLIQARLDALVGEMTADRKTAFDDLARHVVELGERIRRGNLK
                     D"
     gene            1910..4144
                     /locus_tag="PK264_21015"
     CDS             1910..4144
                     /locus_tag="PK264_21015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD78385.1"
                     /translation="MSDHTVNKLKDLLFDRERSELEELSRRIRALHDRVGDDAHLRAS
                     VATVIDGALRDAEVARHRELADAIAPVVLRTVKTEIASSQDLLVESLYPRLGDLVRRY
                     VVSTFRDFVDDLNRRLESKLPFETWSLKLRSIASGRSMAELALSDTSKFAVEEIYLIR
                     RGSGELLEHWERPGLPRSVDDSDHDQRFSGILAAITAFADESFKGDEADLRSFNIGNR
                     LLYIRASAAHLLAAKCRATAPPPIQSILDEEFVATLAEHRDRSRPAASGTPAPSSDAA
                     VAALAGRLETRAREKLLALDGAPSRSGPLKWITALVVLPLLAWFGWQSYIGYQTSVTR
                     QAALQVIGQSIEVKGYPIQLTVERGGHAMSLAGLAPSNEARAIMMRRLRDALPAVAIT
                     DQMNVLPVVPGAPDVGPQFEAMRGAISSLDQAMQRTALTQTIARAAKRLAATGGELQR
                     LEMAAGGPARDVAREARAEIGRVEADLASLTSRIGSATAPADRELVAVQSALTRLSAV
                     FDRLSERGPGSAAKSAPKSIASVADGAEELAALAERIAAAAQGFATTASLRKLEGDIA
                     SLPKPLPPRDDRPLIQALESTVTGLKGELKRLEDELARRPMPTGRAPTPREDLEAFVR
                     SHAIFFANASDYRSDAAAKTTLDELARLMTRSTDVLRIVGYADERGGSTRNVTVSLAR
                     AQRVASELQSRGIPAARLVIVPRSNSIEISANTGPQSTNRRVEFEVGFMGESDADTGA
                     GRAP"
     gene            4141..4653
                     /locus_tag="PK264_21020"
     CDS             4141..4653
                     /locus_tag="PK264_21020"
                     /inference="COORDINATES: protein motif:HMM:NF012300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HRD78386.1"
                     /translation="MTSRKLMLLGEIGVGKSSLVKRVLHNVFTGTYIPTIGVDVHKYV
                     VHDAGPGRDQQVDIVIWDTDGNFGQAIFRHVYIKGASGAMVVADASRPLTIDRMLSLA
                     EGFEDAFPGRPIALVLNKWDLVHDPDRYSTPDGLARTRHSFLRTSAKTGDKVRDAFTT
                     AATIMLRRGA"
     gene            4607..5803
                     /locus_tag="PK264_21025"
     CDS             4607..5803
                     /locus_tag="PK264_21025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRD78387.1"
                     /translation="MRSRPLQPSCSGAEPETPAATSSPDASGIAAAAHFLADRAIFGL
                     VWLDEKLSAVGRFGSIVDFVPEGRPVTDTILPLLGLDSQIRALRRTPQRSLDIPNVSI
                     NLGDSQGPRININVFWSVAERRYLVLVSRVLDRSDLELELAKQVRARSIAESEAAAKA
                     EALVKVNAELERANRELEEFAYVISHDLKAPLRGVRYFIGDLEASLEKADHDEARLCL
                     GRVQAQVRRMSNMLNALLEYARVGRKAEMLETVALAGLAEEIIGSVGAPPGMSLTLDG
                     DWPTIVTLRAPLDLVLRNLVENAVKHHDRPTGRITVRAVDIGAVVSLSVEDDGPGIPP
                     NWHEAIFLPFRKIEDDEAIEGSGIGLALVKKTVELVGGTISVASSPDIARGTIFRVAW
                     PKVIRV"
     gene            5853..>6196
                     /locus_tag="PK264_21030"
     CDS             5853..>6196
                     /locus_tag="PK264_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78388.1"
                     /translation="MKKPTSSAGGDHLEHRSDVTKLRRDAADLKDFLIVEDENFDADR
                     LRATLHIMFGYDISVRRAATLGKAIDSVIERKPNILFLDDVLPPNENAMQTIPFLRKA
                     GYEGPIIIVSGRA"
BASE COUNT         1086 a         2075 c         2038 g          997 t
ORIGIN      
        1 agatcggcgc caaatgaggt ccatatcgac agcgatcaga tgaggagacg accatggcca
       61 ccgtaaagat gctgaccgac gccgaggcga gcccggaggc gcgcgccgtc ttcgacgaca
      121 tccggaagac gcggaagtcc gacttcgtga acaatgcctg gcgggcacag gccaatcatc
      181 cggccatgct gaagcgcaac tgggagcatg ccaagcgcac catggcgccg ggcgcgctcg
      241 atgcgctgac gaaggagttc gtctatctcg ccgtgtcgat catgaacaac tgcgagtact
      301 gcattcactc gcacaccttc ttcgcgcgcc aaaagggcat gacggatgcc cagtactcgg
      361 agttcctcga ggtgatcggc ctcgcgacgg aaggcaatcg catgatgacg gccatgaatg
      421 tgccggtcga cgagcgcttc aaggtgaaga cgacggggat cgacgagtga ggcgccgggt
      481 cctcgccctc agggcagctg cagcctctgt cgcggcgctt gcggcggctt ccgccttcgc
      541 cgccgagccc tctggcgggg gcagcctgcc gctcgccgat gtcatcgagc gcatgaagcg
      601 ctatccgaac ctcgtgcagc agatcaagct ggagctcgta cgggtagggc tgaagcgcga
      661 ggcggtcgtg tgcagcgggc agcgattcgg ccgcgaatgg aaaatgctcg gcggcgagcg
      721 cacgctgccc tatcgctgcg agctcggacg acggcgcctc gcgatcgacg ggcagcgcgt
      781 ctacctcgat gccgccggta agcgcctcga agcctccgac ccgaagctta ctgagcgtgc
      841 ggcccgcgta ctcgaaagcc gcatcacgtg ggtgtggcga tgagcgaggc acgcacgacg
      901 ggccgagccg agcgccctct tgtccgccct cttgcggccc gggacaaaga cgcctggcgg
      961 gcgctcttcg acgcctacat cgccttctat cgcgccgagg ttgcggacgc ggtgatcgag
     1021 gcgacctggg agcggatgct ggccctgtcc cacggcatga cgggcctcgt cgccgtcgat
     1081 ggcggcgacc ggccgatcgg gctcgcgcat cttgtcttcc acgtgtcgac ctggtcgccg
     1141 acgacctact gctacctcga agacctctat gtggcgcctg aacaccaggg ccggggggcc
     1201 ggccatgccc tggtcgaggc gacctacagg gaagctgaag cgcgcgccgc taagcgcgtg
     1261 tattgggtga ccgaagaaga caaccaggcg gcaagacggc tctacgacac gctcggccgg
     1321 cgcatgcatt acgtgcagta caggcgagaa tagcgtccca tttacaacat cggagactaa
     1381 gtgcagcagc acgtcaccgc ccgacttgcc gataagacct ttctcctggg cccttctccg
     1441 attattagta atcttcagga cttagtttcc tggggctgat cgcgtcagcg gctatcgcgc
     1501 gcgcaggaac tgacaaaggt gacgccatga atgccacgac acttggcgag atcagggcca
     1561 gcgccgccgc ccgcgatctc accgacgaca agatgcagca ggttcgcgag ctgctctacg
     1621 gcgagttcaa gcgcgagagc gatgcccgtg tggcgctcct cgagaaccgg gtccgcgagc
     1681 tcgagagcgg gctgcatcgc cggctcgatc tcatccaggc gcgcctcgat gccctggtcg
     1741 gcgagatgac ggcagatcgc aagaccgcct tcgacgatct cgcccggcat gtggtggaac
     1801 tcggcgagcg catccgtcgg ggcaatttga aggactgaga gcgcgtgaca cggccggcag
     1861 ccatcgaact cacagtgatc ggcttgccat gatgccggag cgacacgcca tgtccgatca
     1921 cacggtcaac aagctcaagg atctgctctt cgaccgcgag cgcagcgagc tcgaggagct
     1981 gtcgcgccgc attcgtgcgc tgcatgaccg ggtcggcgac gacgcccacc tgcgtgcgag
     2041 cgtcgcgacc gtcatcgacg gtgcgctgcg cgacgccgag gtcgcgcggc acagggaact
     2101 cgccgacgcg atcgcgccgg tcgtactgcg aacggtgaag acggagatcg cctccagcca
     2161 ggatctgctc gtcgaaagcc tctatccgcg cctcggcgat ctcgtgcgcc gctatgtcgt
     2221 gagcacgttc cgcgacttcg tcgacgacct caatcgccgc ctcgagagca agctcccatt
     2281 cgagacgtgg tcgctcaagc tgcgcagcat cgcgagcggg cgatcgatgg ccgaactcgc
     2341 gctcagcgat acgagcaaat tcgcggtcga ggagatctac ctcatccgcc gcggctcggg
     2401 cgagctgctc gagcattggg agcgaccggg gctgccccgc tcggtcgacg acagcgacca
     2461 tgatcagcgc ttctcgggca ttcttgcagc gatcaccgcc ttcgccgacg agagcttcaa
     2521 gggtgacgag gcggacctca gatccttcaa tatcggcaat cggctgctct acatccgcgc
     2581 ctcggcggct cacctcctcg ctgccaagtg ccgcgccact gcgccgccac cgattcagtc
     2641 gatcctcgac gaggagttcg tcgccacgct cgccgagcat cgcgacagat cgcggccggc
     2701 cgcgagtggc acgcccgcgc cgtcgagcga cgcggccgtt gcagcccttg cgggccgcct
     2761 cgagactcgc gcccgcgaga agttgctcgc tctcgatggc gcgccgagcc gctcgggtcc
     2821 gctcaaatgg atcacggcgc tcgtcgtcct gccgctcctc gcctggttcg gctggcaatc
     2881 gtacatcggc tatcagactt cggtgacgcg tcaggccgcg ctgcaggtga tcggccagtc
     2941 gatcgaggtc aagggatacc ccatacagct caccgtcgag cgaggtggtc atgccatgtc
     3001 gctggccggg ctcgcgccgt cgaacgaggc gcgtgccatt atgatgcgcc gcctccgcga
     3061 tgccctgccg gcggtcgcca tcaccgacca gatgaacgtg ctgcccgtcg tgccgggtgc
     3121 cccggacgtc ggcccgcagt tcgaggcgat gcgcggcgcg atctcctccc tcgaccaggc
     3181 catgcagcgg acggcgctca cgcagacgat cgcccgcgcg gcaaagcgcc tcgcagcgac
     3241 gggtggcgag ctgcagcggc tcgagatggc ggccggcgga cctgccaggg atgtggcgcg
     3301 cgaggcacgc gccgagatag gccgcgtgga ggccgacctt gcctcgctca cctcgcggat
     3361 cgggagcgcg accgcgcccg ccgatcgcga actcgtcgcg gtgcagtcgg cactcacccg
     3421 cctttctgcc gtgtttgatc gcctctccga acgaggccct ggcagcgccg caaagtcggc
     3481 gccgaagtcg attgcaagcg ttgcggacgg tgcagaagag ctggcggcgc tcgccgagcg
     3541 cattgccgcc gccgctcagg gctttgccac cacggcgagt ctgcgcaagc tcgagggcga
     3601 tatcgcgagt ctgccaaagc cactgccgcc gcgcgacgac cggccgctga tccaggctct
     3661 cgagagcacg gttaccggtt tgaagggcga gttgaagcgg ctcgaggacg aactcgcacg
     3721 ccggccgatg ccgacaggcc gcgcgccgac gccgcgggag gacctggagg cgttcgtgcg
     3781 ctcgcacgcc atctttttcg ccaacgccag cgactatcgc agcgacgccg ccgccaagac
     3841 gacgctcgac gagcttgccc gcctgatgac gcgctcgacc gacgtgcttc gcatcgtcgg
     3901 ctatgccgac gagcgcggcg gctcgacacg aaacgtcacg gtgagccttg cccgcgctca
     3961 acgcgtggcg agcgagctgc agagtcgtgg gattcccgct gcgcgcctcg tcatcgtgcc
     4021 gcgctccaac agtatcgaga tcagcgccaa cacagggccg cagagcacga atcgtcgggt
     4081 ggagttcgaa gtcggcttca tgggagagtc tgacgccgac acgggcgcgg ggcgggcgcc
     4141 gtgacctcga gaaagctgat gctgctcggc gagatcgggg ttggcaagtc ctcgctcgtc
     4201 aagcgcgtgc tccacaatgt cttcacgggg acgtacatcc cgaccatcgg cgtcgacgtc
     4261 cacaagtacg tggtgcacga cgcggggccg ggccgcgacc agcaggtcga catcgtcatc
     4321 tgggacacgg acggcaattt cggccaggcg atcttccggc acgtctacat caagggggca
     4381 tcgggcgcga tggtcgtcgc ggatgcctca cgcccgctca ccatcgaccg catgctctcg
     4441 ctcgcggaag ggttcgagga tgcctttccc ggccggccga tcgcattggt tctcaacaag
     4501 tgggatctgg tccatgatcc ggatcgctac tcaacccccg acggcctcgc ccggacccgg
     4561 cacagcttcc tgaggacgag cgccaaaacg ggtgacaagg tgcgcgatgc gttcacgacc
     4621 gctgcaacca tcatgctccg gcgcggagcc tgaaacgccc gctgccacgt cgtctccgga
     4681 cgcgtcgggg atcgccgcgg cggcccactt cctcgccgac agggcgatct tcggcctcgt
     4741 gtggctcgac gagaagctga gcgcggtcgg ccgtttcggc agcatcgtcg atttcgtccc
     4801 ggaagggcgc cccgtcaccg atacgatcct gccgctgctc ggcctcgaca gccagatcag
     4861 ggcgctccgg cgcacgccgc agcgctcgct cgacatcccg aacgtgtcga tcaatctcgg
     4921 cgacagccag ggcccgcgca tcaacatcaa cgtgttctgg tcggtggcgg agcgccgcta
     4981 cctcgtgctg gtctcgcgcg ttctcgatcg ctccgacctg gaactcgagc tcgcaaagca
     5041 ggttcgcgct cgctcgatcg ccgaatccga ggccgccgca aaggccgagg cgttggtcaa
     5101 ggtcaatgcc gagctcgagc gcgccaatcg cgaactcgag gagttcgcct atgtgatctc
     5161 gcacgatctg aaggcgccgc tccgaggagt gagatacttc atcggcgatc tcgaggcgtc
     5221 cctcgagaag gcagatcacg acgaggcccg cctctgcctc ggccgcgtgc aggcgcaggt
     5281 gcgacgtatg tccaacatgc tgaacgcgct tctcgagtat gcgcgcgtcg ggcgaaaggc
     5341 ggagatgctg gagacggtcg cactcgccgg ccttgccgag gagatcatcg gctcggttgg
     5401 ggcgccacca ggcatgtcgc tcacgctcga cggcgattgg ccgaccatcg tcacgctgcg
     5461 cgcgccgctc gacctcgtgc tacgcaatct cgtcgagaat gccgttaaac atcacgaccg
     5521 gccgacggga cggatcacgg tgcgtgcggt agacattggc gcggtagtca gcttgtcggt
     5581 cgaggacgat ggccccggca ttccgccgaa ctggcacgag gcgatcttcc ttcctttccg
     5641 caaaatcgag gatgacgaag cgatcgaggg ctccggcatc ggtctcgcgc tcgtcaagaa
     5701 gacggtggag ctcgtcggtg gcacgatttc ggtggcatcc tcgccggaca tcgcgcgcgg
     5761 gacgatattc cgtgttgcgt ggccgaaggt gattagggtc taatcgcgcc cgcgcgccct
     5821 gacccatggc gacttgcact cgggagagtt agatgaagaa gccgacctcg agcgctgggg
     5881 gcgaccacct cgagcaccgc agcgacgtga cgaagctgcg tcgcgacgcg gccgacctca
     5941 aggatttcct catcgtcgag gacgagaact tcgacgctga ccggctgcgc gcgacgctgc
     6001 acatcatgtt cggctacgac atctctgtcc gccgcgccgc gacgctcggc aaggcgatcg
     6061 acagcgtgat cgagaggaag ccgaacatcc tgttcctcga cgatgtgctg ccgcccaacg
     6121 agaacgcgat gcagaccatc ccgttcctgc gcaaggccgg ctacgagggg ccgatcatca
     6181 tcgtgagcgg gcgcgc
//
LOCUS       DAORNH010000284         6125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9541_length_6125_cov_2.328501, whole genome shotgun sequence.
ACCESSION   DAORNH010000284 DAORNH010000000
VERSION     DAORNH010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9541_length_6125_cov_2.328501"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PK264_21035"
     CDS             complement(<1..952)
                     /locus_tag="PK264_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRD78389.1"
                     /translation="MSKTTGNHSLDQTATRRRFLKGSAIAAGAAAGTLAMPNVSRAQT
                     VVWKYQSTWPAKDIFHEFAVDYATKVNEMSGGRLKLEVLAAGAVVPALQMQDAVIAGA
                     LDGGHGVCAYWYGKHKAYSLFGTPPAFGWDAHGFLAWFYYGGGEALYKELVNDTLKLN
                     LVGYLYFPMPTQPLGWFKKEVKSPDDLKNIKYRTVGLSADLFKELGSAVTIMGGGDIV
                     PAMDRGLLDAAEFNNPSSDSLLGFSDVSKFYMLQSYHQAAESFEIIFNKQKVEALPKE
                     LQAILRYAAFAGTADQFARAHDRYSADLDEQLRGIPFRSPY"
     gene            complement(1053..>1273)
                     /locus_tag="PK264_21040"
     CDS             complement(1053..>1273)
                     /locus_tag="PK264_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HRD78390.1"
                     /translation="VSNLKSGAIMLSGGTVPESSFTQADLDSGRVFFVHDGSSASAAG
                     FDVVVTDQTGASSGAPKSVRVVVSRAHA"
     assembly_gap    1274..1443
                     /estimated_length=170
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1444..1697)
                     /locus_tag="PK264_21045"
     CDS             complement(<1444..1697)
                     /locus_tag="PK264_21045"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRD78391.1"
                     /translation="MSVNRGVMVIGQDMALTGEIRNGKLVEVHGVLDGYIASESVVVH
                     PTGRVIGTLRADSAKVNGTLEGTVQVKNLIEIGSTGRVVG"
     gene            2079..2819
                     /locus_tag="PK264_21050"
     CDS             2079..2819
                     /locus_tag="PK264_21050"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017990658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRD78392.1"
                     /translation="MRLQGKVAIVTGAASGMGAATARMFISEGARVMATDVLEAEGRA
                     LASALGPATRFERHDVSSEADWSRVVEATQNAFGRLDVLVNNAGISGSDPNRLSLSTF
                     EQQVAINMRGVFLGMRAVIPIMQRAKSGSIVNISSISGVTGQDFVHMAYNGAKGAVRA
                     MTKAAAVQFGKDGIRVNSVHPGLMPPMRTSRMSADPTVRERLLAAIPMGRTGRIEEVA
                     SANLFLASDEASYITGAELAVDGGYLAQ"
     gene            complement(2826..3188)
                     /locus_tag="PK264_21055"
     CDS             complement(2826..3188)
                     /locus_tag="PK264_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78393.1"
                     /translation="MRIWRPFPVLALALAATLGGCGDSSTVETHASAGSTASANSSGN
                     GGGGGNGSGASSGARGNSGSSGTAVLGFDKTRKLDPKIEAAINTRCDRMWGNDPHREV
                     CVQRHTEMKLEGLASIRR"
     gene            3309..4562
                     /locus_tag="PK264_21060"
     CDS             3309..4562
                     /locus_tag="PK264_21060"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRD78394.1"
                     /translation="MTASRRDVLKGTGTATIAGSVLLGTSRIVQAETARLPAWRYGTG
                     SHATDTVLMFRGNPAHTFYGTGPIPDRPRIRWQYRTAEIRNVVRGTPMTWTGTGWTGT
                     AAKLADYIYVGSVGGYVYCFEALSGKLVWSHKGGGMFKSSVCLFDNKVYIGNTDDLLR
                     CLDAATGRVVWTVNTGRDLDSSPVVTGGRLYIAGENGYARCLDPQTGREIWKTFMGGI
                     GPGSVPGSNGSETTPAIADGDYYAITYDGDLFCLDTKDGRVKWKAKTHDDTDASPVVS
                     GDLVYAAAEEKASHVYCFERSAGREVWRYSANPRGYWSTPAVAGGKLYVGGDDARLHC
                     LDARSGKGLWMFPTGEAIWSSPAVVDDRVLFGSRDGHLYCVDAGSGREIWRLKLDGRI
                     ISSPCIVDGLIWIGTATGWFYCIGA"
     gene            4592..4786
                     /locus_tag="PK264_21065"
     CDS             4592..4786
                     /locus_tag="PK264_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78395.1"
                     /translation="MVAIAAVMLTFGTARPTGAAPLTMIASESGQRLVEQVRRCPPGY
                     RKTMTGCTRIRPRWWELLIL"
     gene            complement(4820..5464)
                     /locus_tag="PK264_21070"
     CDS             complement(4820..5464)
                     /locus_tag="PK264_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78396.1"
                     /translation="MDVELQRLQLWQLLKREFPEWYGVRAREAARIRAERKDEAAVRR
                     FLAEQLVILRRQNSLHALAASPGKLKVMAKAFHENLVSLSKHSTEACFGFISQGELNS
                     VVLGLAPTSSEGTQLHAQLVSLFEAIIDGRRAPKTYRAPTAEDYKLLETQLVARGWSQ
                     TDLRLFEDAQALMQTPPQKVCKMVQDWFAAQLAVADPEVQVRLLVEALRPAVAG"
     assembly_gap    5899..5911
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          983 a         1926 c         2015 g         1018 t
ORIGIN      
        1 gatacgggga gcgaaaaggg atgccacgca gttgctcgtc gaggtcagcc gaatagcggt
       61 cgtgcgccct ggcgaactgg tcggccgtgc cggcgaacgc ggcatagcgc aggatcgcct
      121 gcagctcttt cggcagggct tcgaccttct gcttgttgaa gatgatctcg aagctctcgg
      181 ccgcctgatg gtagctctgc agcatgtaga acttggagac gtccgagaag ccgagcaggc
      241 tgtcggagga gggattgttg aactcggctg cgtcaaggag gccgcggtcc atggccggca
      301 cgatgtcacc accgcccatg atggtgacgg cggagccgag ctccttgaag aggtccgcgg
      361 agagaccgac tgtgcggtac ttgatgttct tcaggtcgtc gggcgacttg acctctttct
      421 tgaaccagcc gagcggctgc gtcggcatcg ggaaatagag gtagccgacg agattgagct
      481 tcaaggtgtc gttgacgagc tccttgtaga gcgcctcgcc accgccgtag tagaaccagg
      541 cgaggaagcc gtgtgcgtcc cagccgaacg ccggtggcgt gccgaacagc gagtacgcct
      601 tgtgcttgcc gtaccagtag gcgcaaacgc catggccgcc atcgagggcg ccagcaatca
      661 cggcatcctg catctgcagc gcgggcacca cggcgccggc ggccaacacc tcgagcttca
      721 ggcggccgcc cgacatctcg ttgaccttgg tcgcgtagtc gacagcgaac tcgtggaaga
      781 tgtccttcgc aggccaggtg gactggtact tccacacgac ggtctgcgcg cggctgacat
      841 tcggcatcgc cagcgtacca gcggccgcgc cggcagcgat cgcgctgccc ttcaggaaac
      901 gccgccgcgt ggcggtctgg tcgagcgaat gatttccggt tgtcttgctc atctagtcct
      961 cccatgcgcc ttgggccggc ggggtctggc ccggtcgcgg ggcaatctag cccggaagac
     1021 gtcagcggct tcaatagaaa tttgcgtgat cgtcaggcgt gagcccgcga gacgacgacg
     1081 cgcacggatt tcggcgctcc cgagctggca ccggtctggt cggtgacgac aacgtcgaag
     1141 ccggcagcac tcgcgctcga gccatcgtgc acgaagaaga cgcgaccgga gtcgaggtcg
     1201 gcctgcgtga agctactctc cggcacggtg ccgccggaga gcatgatcgc gccgctcttg
     1261 agattggaga cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnntggccca ccacgcggcc cgtcgagccg atctcgatca ggttcttgac ctggacggtg
     1501 ccttcgagcg tcccgttcac tttggcgctg tcagcacgca acgtgccgat cacgcgtccg
     1561 gtcgggtgca cgacgacgct ctccgaggcg atgtagccgt cgagcacgcc gtgcacctcg
     1621 acgagctttc cgttcctgat ctccccggtc agcgccatat cctggccgat gaccataacc
     1681 ccccgattga cgctcactgt ccggctcctt cgtcaccagc gcgtgcaccg ctcgagacag
     1741 tacgcagacc gctgcccttc cgcaatcgat gcccgttcga gtggtgactg atcccagcca
     1801 accgaggctt cgcctcgtaa gtatgcggca gttggccgtt atcactcttt tcgtgcgcgc
     1861 ggccgccagt gctgagtttg cgggcctcca cccactccac cccgcgcgcg gcgcgaagct
     1921 acgaatggtc agaccattcg tggctccgta tgagtggggc acacaatggg cgctgcgccg
     1981 tggcagcttc gcggcgctgt aatggccgct tctggcgtcg gagccgccga gatggcaaag
     2041 tcaacctcga cctcccagga acggtaatgg agcgtgagat gcggcttcaa ggcaaggttg
     2101 cgatcgtgac cggcgcggcc tcgggcatgg gcgcggcgac cgccaggatg ttcatcagcg
     2161 agggcgcacg ggtgatggca accgacgtgc tcgaggccga gggccgtgcg ctcgccagcg
     2221 cacttggccc ggccacccgc ttcgagcgcc atgacgtctc gagcgaagcc gattggtcgc
     2281 gggtggtcga ggcgacgcag aatgccttcg ggcgcctcga cgtcctcgtg aacaatgctg
     2341 ggatcagcgg ctcggacccg aacaggctga gcctctcgac cttcgagcag caggtcgcga
     2401 tcaacatgcg cggggtcttc ctcggcatgc gcgcggtcat cccgatcatg cagcgcgcca
     2461 agtcgggctc gatcgtcaac atctcgtcga tctcgggagt gacggggcag gacttcgtgc
     2521 acatggccta caacggcgca aagggggccg ttcgagccat gaccaaggct gctgccgtcc
     2581 agttcggcaa ggatggcatc cgcgtgaatt cggtgcatcc ggggctgatg ccgccgatgc
     2641 gtacgtcccg gatgagcgcc gacccgacgg tgcgcgagcg cctgctcgct gcgattccga
     2701 tggggcgaac ggggcgtatc gaggaggtcg cgagcgcaaa cctcttcctc gcctcggacg
     2761 aggcctccta catcacgggt gccgagcttg ccgtcgatgg cggctatctc gcgcagtgag
     2821 aggtatcagc ggcggattga cgcgaggcct tcgagcttca tctcggtgtg ccgctgcacg
     2881 cagacctcgc ggtgcggatc gttgccccac atgcggtcgc agcgcgtgtt gatggccgcc
     2941 tcgatcttgg gatcgagctt cctcgtcttg tcgaagccga ggacggccgt gccgctggaa
     3001 cccgaattgc cgcgcgcgcc actcgaggct ccactgccgt ttccgccgcc gccgccattg
     3061 ccgctcgagt tcgctgaggc ggtgctgccc gccgaggcat gggtctcgac cgtcgagctg
     3121 tcgccgcagc caccaagcgt tgcggcaagc gcgagcgcca agacgggaaa gggccgccag
     3181 atacgcatgc tacggtcctg caggttgcgg ggattgtcag cacccgcgta acacagttac
     3241 gacttcgaca actgacagac tgtgagcgtc gcgagttaac aaatggtaaa tgcccggagg
     3301 ctctccccgt gaccgcatcg aggcgcgacg ttctgaaggg taccggcacg gccacgatcg
     3361 ccggttcggt attgctcggc acctcccgaa tcgtgcaggc cgagacagca cggctgccgg
     3421 cgtggcgcta tggcaccggc agccacgcaa ccgacaccgt gttgatgttc cgaggaaatc
     3481 cagcacatac gttctatggc accggtccga tccccgaccg gccgcgcatc cgctggcagt
     3541 accggacggc cgagatccgc aacgtggtgc gtggcacgcc catgacgtgg accggcacgg
     3601 gctggaccgg aaccgcggca aagcttgccg attatatcta cgtcggctcc gtcgggggct
     3661 atgtgtactg cttcgaggcg ctctccggaa agctcgtgtg gagccacaag ggcggcggca
     3721 tgttcaagag ctccgtctgc ctcttcgaca acaaggtcta catcggcaat acggacgatc
     3781 tgctgcgctg tctcgatgcc gcgacgggcc gcgtcgtgtg gaccgtcaac accggacgcg
     3841 acctcgattc ctcccccgtc gtgacaggcg ggcgcctcta cattgccggc gagaacggct
     3901 atgcgcgctg cctcgacccg cagaccggcc gcgagatctg gaagaccttc atgggcggca
     3961 tcggaccggg ctcggtgcca ggctccaacg gctcggagac gacgcccgcg atagccgacg
     4021 gcgactacta cgcgatcacc tacgacggcg acctcttctg cctcgacacc aaggacggcc
     4081 gcgtcaagtg gaaggccaag acgcacgacg ataccgacgc ttcgcccgtc gtcagcggcg
     4141 atctcgtcta tgcggccgcc gaggagaagg cgtcgcacgt ctactgcttc gagcgcagtg
     4201 ccgggcgcga ggtctggcgc tactctgcca atccgcgcgg ctactggtcg acgccggctg
     4261 tcgcgggcgg caagctctac gtcggcggcg atgatgcgcg cctccactgt ctcgatgcca
     4321 gatcaggaaa gggtctttgg atgttcccga ccggcgaggc gatctggtcg tcgccggcgg
     4381 tggtcgacga tcgagtcttg ttcggatcgc gcgacgggca cctctattgc gtcgatgcgg
     4441 gctccggccg cgagatctgg cgcctgaaac tcgatggacg tatcatctcc tcgccgtgca
     4501 tcgtcgacgg tctgatctgg atcggcacgg cgacggggtg gttctactgc atcggagctt
     4561 gagagcgatg acgcctgctt cgagatcgct gatggtcgcg attgccgcgg tgatgctgac
     4621 attcggcacg gcgcgtccca caggcgcggc acctctcacc atgattgcct ccgagagcgg
     4681 ccagcgcctc gtcgagcagg tccgtcgctg cccgcccggc tatcggaaaa ccatgaccgg
     4741 ctgtacccgg atccgcccgc gctggtggga gctgctcatc ctctgacggt gggcgcgcgg
     4801 agccgtcgag cagtgtatct caacctgcaa cggccgggcg cagggcctcg acgagcaggc
     4861 gaacctggac ctcggggtcc gccacggcga gctgggccgc aaaccagtcc tgcaccatct
     4921 tgcacacttt ttgcggcggt gtctgcatga gggcctgggc gtcctcgaag aggcgaagat
     4981 ccgtctgcga ccagccgcgc gcgacaagct gggtctcgag cagcttgtag tcctcggccg
     5041 tcggcgcacg gtacgttttc ggcgcgcgac gcccgtcgat aatcgcctcg aagagtgaaa
     5101 cgagctgagc gtgcagctgc gtcccctcgc tgctcgtcgg cgcaaggccg agcacgacgg
     5161 aattgagctc gccctgcgag atgaaaccga agcaggcttc ggtgctgtgc ttcgacagcg
     5221 agacgagatt ctcgtggaag gccttggcca tgaccttgag cttgccgggg ctcgcggcga
     5281 gcgcgtgcag cgagttctgc cgacggagga tgacgagctg ctcggcgagg aaccgcctga
     5341 cggcggcctc gtccttccgc tctgccctga tgcgcgccgc ctctcgtgcg cggaccccgt
     5401 accactccgg gaactcgcgc ttcagcagct gccagagctg caagcgctgc agctcgacgt
     5461 ccatgacctc gatcgtggcc ggctggcttt gcaggcgcgg gctgaggctt ggccgatccc
     5521 gcgggtggct gcgcggcagc cgtcttcgtc gaggccgatg gcgatgcggt ggcggcactc
     5581 tcgaccgtcg gtcgcggccc gaacaggccg ctcgagagga cgtaggcacc ggcagcgaca
     5641 gcgccgatca cggcgacagc ggcgctcacc accgccatgc gccgccatcg gagcacggtc
     5701 gaatggcgaa tgcccggatg cggccggccc gctgcggcac ctgcagatgg agcctgcctc
     5761 gtgcgtggtg ccggtgcggc ggacggctga ggccggggcg ccggctgcgg ctcagggcgc
     5821 gacatctgag gtgaagttgc ttgtggcaac gggcgcgcgt caggtggcgg ctgcagatgc
     5881 tctggaaaga gcaggtccnn nnnnnnnnnn ncggagccgg gcctccgggc gcaacggtct
     5941 tgtagcgagc gagcgcatcg gcagccgcgt ccgcgacgga acggtcgggc acgtcggccg
     6001 gcccggggat caacaaatcc tcgggccgaa ggggcacatc ggcaggttgc actgggtgca
     6061 cgcgcgctgc ggtagccgcg tgcagatcgg caggactgat tccgacgtcc ggcgtcggcg
     6121 atggc
//
LOCUS       DAORNH010000285         6103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9584_length_6103_cov_3.050926, whole genome shotgun sequence.
ACCESSION   DAORNH010000285 DAORNH010000000
VERSION     DAORNH010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9584_length_6103_cov_3.050926"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PK264_21075"
     CDS             complement(<1..417)
                     /locus_tag="PK264_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRD78397.1"
                     /translation="MKFEGTKSYVATEDLKVAVNAAITLERPLLIKGEPGTGKTVLAI
                     EVAKALNLPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDERVKDIKHYIKRGKLWS
                     AFTHDTRPVLLIDEIDKADIEFPNDLLQELDRMEFYV"
     gene            complement(475..1167)
                     /locus_tag="PK264_21080"
     CDS             complement(475..1167)
                     /locus_tag="PK264_21080"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78398.1"
                     /translation="MATVLITGANRGIGLALATVLANRGDTVIATAREPAKAAALAEL
                     KAKSGRVEIAPLDVTSDESLAALGRTLSGRSIDLLIANSGVNDARGGNDDPANTSATW
                     ARLLGINVIGAFQSIRTLLPCVAKSPAGKIAIISSVMGSSARVAGSGYPYRASKAAIA
                     NVGANLAVELKPKGIAVGIYHPGWVKTDMGGQRADIGVEESSRGLVARFDALSLATTG
                     VFEDYNGQTIPF"
     gene            1390..1728
                     /locus_tag="PK264_21085"
     CDS             1390..1728
                     /locus_tag="PK264_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78399.1"
                     /translation="MRKLATGLTAAVAVSALVIGISTDADAQKKGSAQTCVNKGGTGT
                     GSTDDSARFQAWEAVLQSIDWGSWGAWMTSSKTKIGEAPGYTVRNVKQKCVAGGGLGR
                     QCTIFATLCK"
     gene            1868..2923
                     /locus_tag="PK264_21090"
     CDS             1868..2923
                     /locus_tag="PK264_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HRD78400.1"
                     /translation="MTQEPEVLIRRIGRAGRITLNRPKALNALGLAMVREIWAALLAW
                     KDDPTIHHIVLDGTGGRALCAGGDVRWLYDGRVEGSKHARAFWTEEYRLNALIGSYPK
                     PFIPLMDGIVMGGGIGLSAHSTGLRVVTERSRLAMPETSIGLIPDVGGTWLLAKAPAE
                     SGVYLGLLGEQFGAADAIYAGFADWHVPSGRLSELTVALEADPRPALEIVGGFATSPG
                     DAPLSGEKKAIISACFRHDSVEAILTALKSTGGAIAEKALADLATRSPKSLRMTLAAI
                     RNAHHLPSLARALEIEYRLCVRMFEDGEFIEGVRAAIVDKDRSPKWRPATLAEVTPEV
                     VAHYLGPLPAGEDIVVG"
     gene            3082..3414
                     /locus_tag="PK264_21095"
     CDS             3082..3414
                     /locus_tag="PK264_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78401.1"
                     /translation="MTKNASPEARLPDHLVSGAELRRCWVQLHIDWHKRYDEGSDSVR
                     LGMLQATFVRQLWYGGDGEIVHRAPRIDVEEAKKLYPDFEKHVDGIADALAHAFDETK
                     EEVLKWFV"
     gene            complement(3907..4602)
                     /locus_tag="PK264_21100"
     CDS             complement(3907..4602)
                     /locus_tag="PK264_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78402.1"
                     /translation="MKVAVVSAGGSGMGAEVARRLSKDGFKVAILSLSGKGEALAKEL
                     GGIGVTGSNQSADDLQRLVDQVMAAWGRIDVLVNSAGHGPRDKLLSITDEQWRTGMDV
                     YFLNVVRPTRLIAPTMAAQKGGAIINISTAWAFEPSAMFPTSAVFRAGLAAFTKLFAD
                     QYAADNVRMNNVLPGWIDSLPTTEERRKSVPMQRYGTSAEIAATVAFLASPGAGYITG
                     QNLRVDGGLMRSV"
     gene            4789..>5545
                     /locus_tag="PK264_21105"
     CDS             4789..>5545
                     /locus_tag="PK264_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD78403.1"
                     /translation="MTKRALLSTGFMMPLIAEQLEAAFEVHWLHKIADQAKFLKEVGP
                     RIEAGCTGSHTAGRFSKEVLDALPKLRVIGNFGVGYDTINVAEAAKRGIVITNTPDVL
                     NEEVADTAIGLLLATIREFYKAESYLRDGKWATSGEYRLTPASLRDRKVGIIGYGRIG
                     KAIGRRLEAFGVPLAYFARRRQADVKHTYYSDLTAMARDVDTLIAILPGGPSTQGLVN
                     AEVLAALGPRGIFINMARGTVADEPALIAALANK"
     assembly_gap    5546..5568
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5569..5757
                     /locus_tag="PK264_21110"
     CDS             <5569..5757
                     /locus_tag="PK264_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRD78404.1"
                     /translation="LNEPRIDPRFMTLDNVTLLPHVGSASQHTRNKMGQLTVDNLLAY
                     NEGKPPLTPVPETPFKGW"
     gene            5917..>6103
                     /locus_tag="PK264_21115"
     CDS             5917..>6103
                     /locus_tag="PK264_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRD78405.1"
                     /translation="MRSSIAVLAVLACGLVFSADPALAQSSWSGGTPVYDSDVRKKAD
                     DAEAAREERRKARPPVIY"
BASE COUNT         1061 a         1964 c         1996 g         1059 t
ORIGIN      
        1 gacgtagaac tccatgcgat cgagctcctg gaggaggtcg ttcgggaatt cgatgtcggc
       61 cttgtcgatt tcgtcgatga gcagcacggg gcgcgtgtca tgcgtgaacg ccgaccagag
      121 cttgccgcgc ttgatgtagt gcttgatgtc cttgacgcgc tcgtcgccga gctggctgtc
      181 gcggaggcgg ctaacggcgt cgtactcgta gaggccctgc tgcgccttcg tggtggactt
      241 gatgtgccat tcgagcagag gcaggttcag cgccttcgcc acctcgatag cgagcacggt
      301 ctttcccgtg cccggctcgc ccttgatgag cagcggccgc tcgagcgtga tcgccgcgtt
      361 gacggcgacc ttcaggtctt cggtcgcaac gtaggactta gtgccctcga acttcatggg
      421 ctcggcactc cgcagattgg gttgatgtcg atcgacgctc agaaggggat ccgctcagaa
      481 cggaatagtc tggccgttgt aatcctcgaa gacgccggtt gtcgcaagcg agagggcatc
      541 gaagcgggct acgaggccgc ggctgctctc ctcgacgccg atgtcggcgc gttgtccgcc
      601 catgtcggtc ttcacccagc ccggatgata gatgccgacg gcgatgccct tgggcttgag
      661 ctcgacggca aggtttgcgc cgacgttggc gatcgccgct ttcgacgcgc gataggggta
      721 gccggagcct gcgacgcgtg ccgacgatcc catgacggac gaaatgatgg cgatcttgcc
      781 ggcgggcgat ttcgcgacac aggggagcaa cgtgcggatc gactggaagg cgccgatcac
      841 attgatgccg aggagcctcg cccaggtggc ggacgtattg gccggatcgt cgttaccgcc
      901 gcgggcatca ttgacgccgg aattcgcaat gaggagatcg atgctccgcc cggacagggt
      961 gcggccgagc gcggccaggc tttcgtccga cgtgacatcg agcggcgcaa tctcgacccg
     1021 ccccgacttc gccttgagct cggcgagggc agctgccttc gccggctccc gcgcagtcgc
     1081 gatgacggta tcgccgcggt tggcgagaac ggtcgcaagc gccaggccga tgcctcggtt
     1141 ggcgccagtt atgaggacgg ttgccatgtc gtcgcgcggt ccttcggcgt ctggttgatc
     1201 gaacgagttc gcggcgacca tagccgcact cggccgaggc ggcaaagcac cggctgccgc
     1261 atacgtgcca cgctgaaccg gaaaagcgct cggcgatcga gtttggttaa ccgtgccgca
     1321 tgggcaagcc ggacgtgccc gtccacggtc gggtgctgac gaatcactcg cacagggaag
     1381 gattgcttca tgcgcaagct cgccaccggc ctcaccgctg ccgtcgccgt ctcggcgctg
     1441 gtcattggca tctcgaccga tgccgacgcc cagaagaagg gttccgcgca gacctgcgtg
     1501 aacaagggcg gcacaggcac cggctcgacc gacgactcgg cgcgcttcca ggcctgggaa
     1561 gcggtcctgc agtcgatcga ttggggcagc tggggcgctt ggatgacgtc gtccaagacg
     1621 aagatcggcg aggcgcccgg atacacggtg cgcaacgtga agcagaagtg cgtcgccggc
     1681 ggcgggctcg gccgccagtg cacgatcttc gcgacgctct gcaagtaggg ctagcgaacg
     1741 acgcttcgcg atcgatatcg gggcgccggg ctttcgagca cggcgcctgt tgtagtgtcc
     1801 ggagtagcgc agccccatct gaggcgaccc ttgacgcctc cggcgctgcc gcgtacgccc
     1861 gcgccccatg acccaggaac ccgaagtact cattcgccgc attggccgtg caggccgcat
     1921 cacgctcaat cgcccgaagg cgctcaatgc gctcggcctc gccatggtgc gcgagatctg
     1981 ggctgcactc ctcgcctgga aggacgatcc gaccatccat cacatcgtcc tcgacggcac
     2041 gggcgggcgg gcgctttgtg cgggcggcga cgtgcgctgg ctctacgatg gccgcgtcga
     2101 gggctccaag catgcgcgcg cgttctggac cgaggaatac cggctcaacg cgctgattgg
     2161 cagctatccg aagcccttca tcccgctgat ggacggcatc gtcatgggcg gcggcatcgg
     2221 cctctctgcc cactcgaccg gcctccgcgt cgtcaccgag cgctcgcggc tcgcgatgcc
     2281 cgagacctcg atcgggctca ttcccgacgt cggcggcacc tggttgcttg ccaaggcgcc
     2341 cgccgagagc ggcgtctatt tgggcctcct cggcgagcag ttcggcgcgg ccgatgcgat
     2401 ctacgcgggc tttgccgact ggcatgtgcc ttcgggccgg ctcagcgagt tgaccgtggc
     2461 gctcgaagcg gacccgcgtc ccgctctcga gatcgtcggt ggctttgcga cgagccccgg
     2521 cgacgcgccg ctctcaggcg agaagaaagc gatcatctcg gcctgcttcc gccatgacag
     2581 cgtcgaggcc atcctgactg cgctgaaatc cacgggcggc gccattgccg aaaaggcgct
     2641 cgccgatctc gccacccgct cgccgaagtc gctgcgcatg accttggccg cgatccgcaa
     2701 cgcccaccac ctgccgagcc tcgcccgcgc cctcgagatc gagtaccggc tctgcgtgcg
     2761 catgttcgag gacggcgagt tcatcgaagg cgtgcgcgcc gccatcgtcg acaaggatcg
     2821 gagcccaaag tggcggccgg ccacgctcgc cgaggtcacg ccggaggtgg tggcgcacta
     2881 tctcgggccc ttgccggctg gagaggatat cgtggtcggg tgaggcggcg cggcaggaga
     2941 tgggcgctga atgtgacctc gagagtgcga tcggattggg gcaatgagcg gaacaagaga
     3001 gacggctccg caatcgaggt tgttttggaa aaagatgctc gaaggtgagc cgaagaaatg
     3061 aatgcagaaa cgaaccctca cgtgacaaag aatgcgagtc cagaagcacg cctgcccgat
     3121 caccttgtca gcggtgccga acttcggcgc tgctgggtgc agcttcatat cgactggcac
     3181 aagcgctacg atgaaggctc agacagtgtg cggctgggca tgttgcaggc caccttcgta
     3241 aggcagttgt ggtatggggg cgacggcgaa atcgtccacc gcgcccctcg aatcgacgtt
     3301 gaggaggcaa agaagctcta tcccgatttt gagaagcatg tagatggcat agccgacgcc
     3361 cttgcacacg catttgatga aacgaaagaa gaagttctta agtggttcgt atagtcgggc
     3421 tgtgcgcagg aagcatacgc catctgatca tcggtgtctg cgagacgtcg gcaacggccc
     3481 gtcgcgattt tgagaacgaa atcgatcttg ggatgtaggc tgccatcggt gtcgaaagtt
     3541 ctgcaggctc atccttttgc gctgcacacg gcgttggcag atccgtccgg taactttgca
     3601 acatgagtgt tgagcacgcg cactgagtca ctgagtgagc aacggatcaa aagccgaact
     3661 tgcctccgca gtctcggccc cttcggggcc gtggactgtc gtcaagggtg acgcgcagct
     3721 gaaccagcgg gcgcgaggcc ttcgccccgc tggtgcgaag tcgttgcgcg tcctcccaag
     3781 ggatacaccc gcagcgacaa tcgtatgctc ctcaggggcg cacgcagggt cgccttgggg
     3841 gcgttccgcg gacggcgcac tcccgcacat agtggcgtcc gcgatagctg ccaccaactc
     3901 cgccccctac accgaccgca tcagcccgcc atcgacccgc aagttctgcc cggtgatgta
     3961 gcccgcgccg ggggatgcca ggaaggcgac ggtcgcggcg atttccgcgc tcgtcccgta
     4021 gcgctgcatc ggcacggact tgcggcgctc ctccgtcgtc ggcaggctgt cgatccagcc
     4081 tggcagcacg ttgttcatgc ggacattgtc ggcggcgtac tggtcggcga acagcttcgt
     4141 gaaagccgcg aggcccgcgc ggaacacggc cgaggttggg aacatcgcgc tcggctcgaa
     4201 ggcccaggcc gtcgagatgt tgatgatcgc gccgcctttc tgagcggcca tagtgggcgc
     4261 tatcagcctc gtcgggcgga ccacgttcag gaagtagacg tccatgccag tccgccattg
     4321 ctcgtccgtg atgctgagga gcttgtcgcg cggaccgtgg ccggcgctgt tcaccagcac
     4381 gtcgatgcga ccccacgcgg ccatcacttg atcgaccagc cgctgcaggt cgtccgccga
     4441 ctggttggag ccggtgacgc cgatgccgcc aagctccttc gccagcgcct cgcccttgcc
     4501 ggacaacgag aggatcgcga ccttgaagcc atccttggag aggcggcgcg cgacttccgc
     4561 acccatgccg ctgccgccgg ccgatacgac tgctaccttc atcgccgctg ccccatctgt
     4621 tcgcgccgca tcgcgctatt ggccggagga tcgcacgccc cgacgtcggc gagcaatgtc
     4681 ccaaatgctc gcgagacctc cgacagcttc gagaatgtcg ccgcaattgc cggatcgcct
     4741 gcaacgggct acatcacgct gatatctcac caacccttgg agcttcgagt gaccaagcgc
     4801 gcccttctgt cgaccggttt catgatgccg ctgatcgccg agcagctcga ggccgccttc
     4861 gaggtgcact ggctccacaa gatcgccgac caggcgaagt tcctcaaaga ggtcggccca
     4921 cgcatcgaag cggggtgcac cggctcgcat acggcgggtc gcttcagcaa ggaggtgctc
     4981 gatgccctgc cgaaactcag ggtgatcggc aatttcggcg tcggctacga cacgatcaac
     5041 gtcgccgagg cggcaaagcg cggcatcgtc atcaccaata cgccggatgt gctgaacgag
     5101 gaggtcgccg acacagccat cggcttgctg ctggcgacga tccgcgagtt ctacaaggct
     5161 gagagctacc tgcgcgacgg caagtgggcg accagcggcg aataccggct gacacccgcc
     5221 tcgctgcgcg atcgcaaggt cggcatcatc ggttacggcc gcatcggcaa ggcgatcggg
     5281 cggcggctcg aagcgttcgg cgtgccgctc gcctacttcg cgcggcgccg ccaggccgac
     5341 gtcaagcaca cctactactc ggacctcacg gccatggcgc gcgatgtcga tacgctgatc
     5401 gccatcctgc cgggcggacc gtcgacacag ggcctcgtca atgccgaagt gctggcggcg
     5461 ctcgggccgc gcggcatctt catcaacatg gcacgcggca ccgttgccga cgagccggcg
     5521 ctgattgcgg cgctcgccaa caagannnnn nnnnnnnnnn nnnnnnnnct gaacgaaccg
     5581 agaatcgacc cgcggttcat gacgctcgac aacgtgacgc tgctgccgca cgtcggctcg
     5641 gcctcccagc acacccgcaa caagatgggc cagctgaccg tcgacaatct gctcgcctac
     5701 aacgagggga agccgccgct gacgcccgtt ccagagactc cgttcaaagg ttggtgaacg
     5761 ccagctgccg gcaacccaac acaacctgtg cagggatgcc acaggcgtcg ctccggatgg
     5821 cgataagtgt gcttgccgtg cttgtcttgc cgcagttggg ccgcttatct ccttcccgtg
     5881 gcgcgggcat tccggccgtg gcttgaggga ttggtcatga gaagctcgat cgcggttctt
     5941 gcggtgctgg cttgcgggct cgtgttcagc gcagacccgg cacttgccca gtcgagctgg
     6001 tccggcggca cgccggtcta cgacagcgat gtccgcaaga aggccgacga tgccgaggcc
     6061 gcccgcgaag agcggcgcaa ggcgcgcccg ccggtgatct atc
//
LOCUS       DAORNH010000286         6076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9635_length_6076_cov_2.703870, whole genome shotgun sequence.
ACCESSION   DAORNH010000286 DAORNH010000000
VERSION     DAORNH010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9635_length_6076_cov_2.703870"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PK264_21120"
     CDS             <1..479
                     /locus_tag="PK264_21120"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HRD78406.1"
                     /translation="LRAPRPQWQRFLAVWLGAVALYFGVVFTLAFTVGPKIAAGVNGK
                     FWPRLMASEWAFIAGLVAIALMAFALIASWYRLGTLRELVASARYAGRRWTLDVPASE
                     FVQLAAGNGLLRVLTLGQAGPYTEARMLRLLVRNLSEHLPGEGDESRRGDPECDAA"
     gene            complement(411..>1733)
                     /locus_tag="PK264_21125"
     CDS             complement(411..>1733)
                     /locus_tag="PK264_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRD78407.1"
                     /translation="GVVMARDLENLQDRLPPFPDREARRAIEEALGGRLEDHFAEFGP
                     PIAAASIAQVHKAVVIDGGERRTVAVKILRPSIERRFKRDLDAFYFAARQIERFHEPS
                     RRLRPVAVVDTLAHSVELEMDLRLEAAAISEMAENIAKGGDQGFRVPRVDWRRTAKRV
                     LTLEWIDGPSIADRQALDALGLDRRALGLTVLQTFLRHAMRDGFFHADMHQGNLLVER
                     DGTIVAVDFGIMGRLGQRERRFLAEILHGIITRDYQRAADVHFWAGYVPEHHPRAVFA
                     QALRAIGEPIHGRTAAEISMADLLGQLFAYTEVFDMRTRPELLMLQKTMVVVEGVARG
                     LDPSLNIWEAIARAWVEENLGPAGALKDAREGAGDVGRFLSAVPALLAGAETTLAAVT
                     RIADQGLRLDDDTVRRLASAEARATRSGRIALWVAAAALVALAWQMLR"
     assembly_gap    1734..1743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1744..2041)
                     /locus_tag="PK264_21130"
     CDS             complement(<1744..2041)
                     /locus_tag="PK264_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HRD78408.1"
                     /translation="MPQAMRSILALLRAGLVLAQHGVRLVPKGMPPPLPLRLAHIATA
                     PLRWLSAPFRIGQPKDRRISGALGRLGPSYIKLGQFLGTRGDIVGPEVSADLRHL"
     gene            complement(2127..3338)
                     /locus_tag="PK264_21135"
     CDS             complement(2127..3338)
                     /locus_tag="PK264_21135"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78409.1"
                     /translation="MTISTGESGHGSAAARAYAVRVAIFYAAVFLVAGAQMPFLPVWL
                     DWNGLTASEISVVAAVPLIVRIIVTPAFAFLADRHGDHRLALIALSWAGFAMLLLLTR
                     AGGFWPILISATLFALFWTSVMPLTETIALNGVRRAGLDYGRMRIWGSISFIAASFGG
                     GFILEWTGNAFAIWLIVAGGLATVIGAHLLPQPGSLPNTTAGAAASATARKGLRLDEA
                     LALLSNRAFLTFLLAVGSIQAAHAVIYTYGTLHWQSQKITSATAGMLWTIGVIAEIML
                     FAWSKAIVARLGPMLLIAIGGVAAVVRWTIMAFDPPVALLVPLQILHALTFGATHLGA
                     VHHIAATISERQGATAQAVTAAVTAGIAMGGATLLSGRFYEAFQGRAYLAMAALGAIG
                     LAAALVLRRAR"
     gene            3427..4038
                     /locus_tag="PK264_21140"
     CDS             3427..4038
                     /locus_tag="PK264_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78410.1"
                     /translation="MQRPEPPPPQDADKEFRAIEAALLETARGRWFLAEHGRRSRRME
                     TSALETAIGQLKSSLRAPPAVVGRIKSDLQALVEEVTTARSAVMQRVPVPAKDSPEVN
                     GTHAPQGILQAAEELHELAWALQSRDVDAETCTRIGRQAASIFALAAQQSQESQRARR
                     FSEALDRLMERLQATLQAVDQEQQAPGEDAEAIPASAIKAAVA"
     gene            4110..5360
                     /locus_tag="PK264_21145"
     CDS             4110..5360
                     /locus_tag="PK264_21145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD78411.1"
                     /translation="MPEKTAKPADGRMLPLDGVKVVDISNFLAAPMSAMFLADFGADV
                     VKVERPGQGDEIRYWGRNKGGVGLYYKIVNRNKRSVTADLRTPFGVEVVKRLVKSADI
                     LVENYRVGTLEKWGLGPDVLHAINPGLVIVRVTGFGQTGPYRKRPGFGTLAEAFAGFV
                     HINGTPDRPPLLPGFGLADSTTGLMAAYLASLALHERRRSGKGQVIDLAIYETLFKLL
                     GPQAIDYDQLGEVQQRAGSRLPFTAPRNTYRTRDDKWVSIAGSAQSTFERMCAALEIP
                     DVPRDPRFADNRLRLKHAAELDEALQGAIERFDQADLMARFEKAEAAIAPVNDIAQIF
                     ADPQFQARENIATVEDEELGPVRMQNVVGKLSATPGRISKAGPKLGRHNREVLIGELG
                     FSETELAAAGITIDKASGKRAAAE"
     gene            5377..>6076
                     /locus_tag="PK264_21150"
     CDS             5377..>6076
                     /locus_tag="PK264_21150"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRD78412.1"
                     /translation="MKTRRFGRTGLAISEITYGGGWVGGYIIRAGEAERHTILNRALH
                     AGINWVDTAALYGNGASETVLGQWLPTVPANRRPRLSTKFNVDVTAGDFKGQIEASVK
                     ASLARLKLPRVEVLILHNRVVDEGRKDRDPRSLHPAEVLGRGGIADIMDGLRRAGLCD
                     WIGLTGLGDPAALAQVVGSGRFDVMQGYYNLLNPSGVMPTGPGWSSTDFNGLLKLARA
                     KDVGVMGIRIFAAGH"
BASE COUNT         1032 a         1996 c         2141 g          897 t
ORIGIN      
        1 tcttacgggc gccgcgtccc cagtggcagc gcttcctcgc agtctggctc ggggcagtcg
       61 ccctctactt cggcgtcgtg ttcacgctcg ccttcacggt gggtccgaag attgcggctg
      121 gggtgaacgg caaattctgg ccacggctca tggcctcgga gtgggccttc atcgcgggtc
      181 tcgtcgcgat tgccctgatg gcctttgctc taatcgcgag ctggtatcgc ctcggcacgc
      241 tgcgcgagct cgtcgcgtcg gcgcgctatg ctggccgccg ctggacactc gatgtgccgg
      301 cgtccgagtt cgtgcaactc gcggccggaa atggcctgct gcgagtgctg acgctcgggc
      361 aggcaggccc ttacaccgag gcacggatgc tccgcctgct ggttcgcaac ctatcggagc
      421 atctgccagg cgagggcgac gagagccgcc gcggcgaccc agagtgcgat gcggcctgac
      481 cgggttgcgc gcgcttccgc cgaggcgagg cggcgcacgg tatcgtcgtc gaggcgcagg
      541 ccctggtcgg cgatccgcgt cacggctgca agggttgtct cggcaccagc cagcagcgcc
      601 ggaactgcgc tgagaaagcg gccgacgtcg ccagcacctt cgcgcgcatc cttgagcgcg
      661 cccgccggcc cgaggttctc ctcgacccag gccctcgcga tcgcctccca gatgttgagg
      721 ctcggatcga ggccgcgcgc aacgccctcg acgaccacca tggtcttctg gagcatgaga
      781 agctcgggcc gtgtccgcat gtcgaacacc tcggtgtagg caaagagctg cccgagcagg
      841 tcggccatcg agatctcggc ggcggtgcgt ccgtggatcg gctcgccgat cgcgcgcaac
      901 gcctgcgcga acacggcacg gggatggtgc tcgggcacgt agccggccca gaagtgcacg
      961 tcggcggcgc gctggtagtc gcgggtgatg atgccgtgca ggatctcggc gaggaagcgg
     1021 cgctcgcgct ggccgagcct gcccatgatg ccgaaatcga cggcgacaat ggtgccgtcg
     1081 cgctcgacga gcaggttgcc ctggtgcatg tcggcatgaa agaagccgtc gcgcatcgcg
     1141 tggcggagaa acgtctgcag tacggtgagg ccgagcgcgc ggcgatcgag acccaaggcg
     1201 tcgagggcct ggcggtcggc gatcgagggg ccgtcgatcc actcgagggt gaggacgcgc
     1261 ttggccgtgc gccgccagtc gacccgcggc acgcgaaagc cctggtcgcc gcccttggcg
     1321 atgttctccg ccatctcgga gatggcggcc gcctcgaggc ggagatccat ctcgagctcg
     1381 accgagtggg caagcgtgtc gacgacggcg accggccgga gccgccgcga cggctcgtgg
     1441 aagcgttcga tctggcgggc ggcgaaatag aaggcgtcga ggtcgcgctt gaagcggcgc
     1501 tcgatgctgg ggcggaggat cttcacggcg acggtgcgcc gctcgccgcc gtcgatcacc
     1561 acggctttgt ggacctgagc gattgaggcg gccgcgatcg gcgggccgaa ctcggcgaaa
     1621 tgatcctcga gccggccgcc gagcgcctcc tcgatggcac gccgcgcttc gcggtcgggg
     1681 aacggcggca ggcggtcctg caggttctcg aggtcgcgcg ccatcacgac gccnnnnnnn
     1741 nnnggaggtg gcggaggtcg gccgacactt cggggccgac gatgtcgccg cgcgtgccga
     1801 ggaattggcc gagcttgatg tagctcggtc cgagccgccc gagcgcgccg gagatgcgcc
     1861 gatccttcgg ttggccgatg cggaaggggg ccgaaagcca gcgcagcggc gccgtcgcaa
     1921 tgtgggcgag gcggagcggc aacggcggcg gcatgccttt cggcacgaga cggacgccgt
     1981 gctgggccag cacgagccct gctcgcagca gcgccagaat gctcctcatc gcctgcggca
     2041 ttccgcggac ctcgcctcgt cgatcatcaa gctcggcttg actgacggca aagcgggcga
     2101 gggtcaagtg ctccggcaac ttatcgtcag cgggcgcgac gcagaacgag cgctgccgcg
     2161 agcccgatcg cgccgagcgc cgccatggcg agataggctc gaccctgaaa ggcctcgtag
     2221 aagcggccgg agaggagcgt cgccccgccc atggcgattc cggccgtaac cgccgccgtc
     2281 acggcctgcg cggtcgcccc ctgacgttcg ctgatcgtcg cggcgatgtg gtgcacggcg
     2341 ccgaggtgcg ttgccccgaa cgtgagcgcg tggagtatct gcaacgggac aagcagcgcg
     2401 acgggcggat cgaaggccat gatggtccag cggacgacgg ccgcgacccc gccgatcgca
     2461 atcagcagca tggggccgag ccgcgcgacg atggccttgg accaggcgaa aagcatgatc
     2521 tcggcgatga ccccgatggt ccagagcatg ccggcggtcg ctgaggtgat cttctgcgac
     2581 tgccagtgca gcgtgccata ggtgtagatc accgcatgcg cggcctgaat cgagccgacg
     2641 gcgaggagaa acgtcaggaa cgcgcggttg gagaggaggg ctagcgcctc gtccaggcgg
     2701 agccctttgc gtgccgttgc cgaggcggcg gcgccagcag tggtgttcgg cagcgagccc
     2761 ggttgcggca acaggtgcgc gccgatcacc gttgcgaggc cgcccgcaac aatgagccag
     2821 atggcgaagg cgttccccgt ccattcgagg atgaagccgc cgccgaagct cgcggcaatg
     2881 aagctgatcg agccccagat ccgcatgcgg ccgtagtcga gcccggcacg cctgacgccg
     2941 ttgagcgcaa tcgtctcggt cagaggcatg acgctcgtcc agaacagggc aaagagtgtc
     3001 gccgagatga ggatcggcca aaagccgccg gcccgcgtga ggaggagcag catggcaaag
     3061 ccagcccacg agagcgcgat cagggccagg cgatggtcgc cgtggcgatc ggcgaggaag
     3121 gcgaaggcag gggtcacgat gatgcgcacg atgagcggga ccgccgcgac gacgctgatc
     3181 tcggaagcgg tgaggccgtt ccagtcgagc cagactggga gaaacggcat ttgcgcgccg
     3241 gcgacgagga agacagcggc gtaaaagata gcaacgcgca cggcataggc gcgtgccgcc
     3301 gcgcttccat ggccactctc tccggtcgaa atcgtcacgc cacgccgctt tcgccgaaga
     3361 tgacctgtgg ggaacttgcg aatcagctat cactgttcga cctcacctat ttgggaatcg
     3421 ccgctgatgc aacggcccga gccgccaccg ccccaggatg cggacaagga gtttcgagcc
     3481 atcgaggcgg cactcctcga gaccgcgcgc gggcgctggt tccttgccga gcatggccgc
     3541 cgttcgcggc gcatggagac gagcgcgctc gagaccgcca tcggtcagct caagtcgtcg
     3601 ctcagagccc cgccggccgt cgtcggccgg atcaagtcgg atcttcaagc gctggtcgag
     3661 gaggtcacga ccgcgcgctc cgcagtgatg cagcgagtgc cggtgccggc caaggatagc
     3721 ccagaggtca atggcacgca tgcaccgcaa ggtattctgc aggccgccga ggagctgcac
     3781 gagctcgcct gggcgctgca gtcgcgcgat gtcgacgccg agacgtgcac acgcatcgga
     3841 cggcaggcgg cgagcatctt tgcgcttgca gcgcaacaat cgcaggaatc tcagcgcgcg
     3901 cgtcgcttct ccgaggcact cgaccggctg atggagcgtt tgcaggcgac actgcaagcc
     3961 gtcgaccagg agcagcaggc acctggcgaa gacgcagagg ccatcccggc gtctgccata
     4021 aaggcagccg tggcctgaca gcattgcggg ttggatgttt cgggccgatc tgctcttatt
     4081 ggggcagctg aagcgcccgg atccgcacca tgccagagaa aaccgccaag cctgccgatg
     4141 gccgcatgct gccgctcgac ggcgtcaaag tcgtcgacat ctcgaacttc ctcgctgcac
     4201 ccatgagcgc catgtttctc gccgacttcg gcgcggacgt ggtcaaggtg gagcgacccg
     4261 gccagggcga cgagatccgc tattggggcc gcaacaaggg cggggtcggg ctctactaca
     4321 agatcgtcaa ccgcaacaag cgctccgtca cggcggacct gcgcacgccc ttcggcgtcg
     4381 aggtggtgaa gcgcctcgtc aagtccgccg acatcctggt cgagaactac cgcgtcggta
     4441 ccctcgagaa atggggcctc ggacccgatg tgctgcatgc gatcaacccc ggcctcgtca
     4501 tcgttcgcgt cacaggcttt ggccagacgg gcccctatcg caagcgtccg ggcttcggca
     4561 cgctcgcgga agcctttgcc ggcttcgtgc acatcaacgg cacgccggat cgtccgccac
     4621 tactgccggg cttcggcctt gcggattcga ccacgggcct catggccgcc tacctcgcct
     4681 cgctggcact ccacgagcgc cgacgctccg gcaagggcca ggtgatcgac ctcgccatct
     4741 acgagacgct cttcaagctg ctcggcccgc aagcgatcga ctacgatcag ctcggcgagg
     4801 tgcagcagcg cgcggggagc cgcctgccgt tcacggcgcc gcgcaacact taccgcacac
     4861 gagatgacaa gtgggtgtcg atcgccggca gcgcgcagtc gactttcgag cgcatgtgcg
     4921 cggccctcga gatccccgat gtgccgaggg acccgcgctt cgccgacaat cgcctgcgcc
     4981 tgaagcacgc cgccgagctc gacgaggcgc tgcagggggc gatcgagcgc ttcgaccagg
     5041 cggacctgat ggcgcgcttc gagaaggcgg aggcggccat cgcgcccgtc aacgacatcg
     5101 cccagatctt cgccgacccg cagttccagg cacgcgagaa catcgcgacc gtcgaggacg
     5161 aggagctcgg ccccgtgcgg atgcagaacg tcgtcggcaa gctctcggcg acgccgggcc
     5221 gcatcagtaa ggcaggtccg aaactcggcc ggcacaaccg cgaggtgctg atcggcgagc
     5281 tcgggttcag tgagaccgag ctcgcggcgg ccgggatcac gatcgacaag gcgagcggca
     5341 agcgcgccgc ggccgaatag tgcgacgggg gacttcatga agacgcgcag gttcgggcgg
     5401 acggggctcg ccatctccga gatcacctac ggcggcggct gggtcggcgg ctatatcatc
     5461 cgcgccggcg aggccgagcg gcacaccatc ctgaacagag cgctgcatgc cggcatcaac
     5521 tgggtcgata cggcggcgct ctacgggaac ggcgcgtccg agacggtgct cgggcaatgg
     5581 ctgccgacgg tcccggcgaa caggcgcccg cggctctcga ccaagttcaa cgtcgatgtg
     5641 acggccggcg atttcaaagg ccagatcgag gcctcggtga aggcgagcct cgcccgcctg
     5701 aagctgccgc gcgtcgaggt gctgatcctg cacaaccgcg tggtcgatga gggtcgcaag
     5761 gatcgcgatc cgcggagcct ccatccggcc gaggtgctgg ggcgcggcgg catcgccgac
     5821 atcatggacg ggctccgccg cgctggcctc tgcgactgga tcggcctgac cggcttgggc
     5881 gatccggcgg cgcttgccca ggtggtcgga agcggccgct tcgacgtcat gcagggctac
     5941 tacaacctcc tcaatccgag cggagtgatg ccgacaggcc ctggctggag ctcgacggac
     6001 ttcaacggcc tcctgaagct cgcgcgtgcc aaggacgtgg gcgtgatggg gatccgcatc
     6061 tttgcggcag gccacc
//
LOCUS       DAORNH010000287         5935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_9935_length_5935_cov_2.335544, whole genome shotgun sequence.
ACCESSION   DAORNH010000287 DAORNH010000000
VERSION     DAORNH010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9935_length_5935_cov_2.335544"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PK264_21155"
     CDS             <1..250
                     /locus_tag="PK264_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HRD78413.1"
                     /translation="RVALPIPGGDVIGGRPLEAMTLVALGYRSLSMAPASIGPVKAMI
                     RSLDVGAAREVLTSLLRTDARSIRREIADFAAAQGVTI"
     gene            265..1296
                     /locus_tag="PK264_21160"
     CDS             265..1296
                     /locus_tag="PK264_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRD78414.1"
                     /translation="MMRGAFTIGGIEVRAGERRLVDLPVAKLSNHTPVTLPVHVLHGL
                     LPGPTLFISAAIHGDELNGVEIIRRVLRTLMPGNISGTLLCVPVVNAFGFIGHSRYLP
                     DRRDLNRSFPGSATGSLAARLANLFLNEVVRRCTVGIDLHTAAVHRINLPQIRADFVK
                     RPRTRELAEAFGAQVVLESPERSGSLRRAAREAGIDVLVYEGGEGLRFDEFAIKAGVD
                     GIANVMLKIGMLQLPDGVEPAASNPAYRPPVFANTSRWVRAPDGGVLRSVRRIGDAVG
                     QGELIGYVANPYEDRELEVRAPHRGIIIGRTTLPVVNMGDALFHIAWSAEHDASAPKE
                     ALMDEDEII"
     gene            1369..1635
                     /locus_tag="PK264_21165"
     CDS             1369..1635
                     /locus_tag="PK264_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HRD78415.1"
                     /translation="MTQTNNRFLDELAKLMTDAAGAAQGARREVETLMRAQGERMLRG
                     MDIVQREEFEAVRAMAVRAREENERLAEKVARLEAEVERLKGPI"
     assembly_gap    1700..1735
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1860..1982)
                     /locus_tag="PK264_21170"
     CDS             complement(1860..1982)
                     /locus_tag="PK264_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78416.1"
                     /translation="MRGGSWNFPAIDARASIRQPLKPDYGVYSIGFRVARDLPN"
     gene            complement(2061..>3771)
                     /locus_tag="PK264_21175"
     CDS             complement(2061..>3771)
                     /locus_tag="PK264_21175"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRD78417.1"
                     /translation="DIRKSLGNAQRANSPEGRVVLYLDACFTGSSGAGWIAAETSSVR
                     ISPSAIRAAEPRVVELAASGSDQEANWDRERRLGIFTDALVEGLYGAADLDRDGHVTA
                     AELSAFLARRIDHRVTRLFPSPRRYQTPSLSGDGAARLASISASTPSRDPEATARLAV
                     RCATVPKSSDAAEVREFLGNCGAECPCLPALEVRLRELETAATACRVEGEELERLTKR
                     GSAARAQIEALAASATCAEVKARAVGVISGIATAPPGTPPTPEARQPSRIVPPAPPPA
                     RGESRVSVKSVVRVRQPFTVPGCGIVIVPTRTARHLGQQVYEGGIAVDGKNMLVRTGT
                     LLTPLSPSGGCKLFVVLRPSGDIELDGECSCSDPSAPPSPGTSAPTPSGTSAPSKFFR
                     DNRLDHTSCTECPEMAVIPVGTFVMGTVEAAGQTERSELPARKISFTKSFALGRHEVT
                     FDEWDACVATKMCTLRPADEGWGRGRQPVVNVTANAISEYLTFLAAITGQSYRLPTEA
                     EWEYAARAGAATRFSTGDTLTAKDANVERSRGRAVPVGSYAANAFGLFDMHGNVWEIV
                     AEC"
     assembly_gap    3772..3858
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3859..4217)
                     /locus_tag="PK264_21180"
     CDS             complement(<3859..4217)
                     /locus_tag="PK264_21180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78418.1"
                     /translation="MSVLARAVLVLTALVVTVLPAAAGPDDIVIIIANQDYRGTVPKV
                     EYADRDGEAFAKAAREVLRVPADRIIVERNLTYAGFLGYFGRDGEGGPELQRLKARLG
                     RASTITFFYSGHGMPAKR"
     gene            complement(4316..>5691)
                     /gene="trkA"
                     /locus_tag="PK264_21185"
     CDS             complement(4316..>5691)
                     /gene="trkA"
                     /locus_tag="PK264_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRD78419.1"
                     /translation="KVIICGAGQVGFGIAERLAAEGNDISIVDTRPDLVQRANDTLDV
                     RAYVGNGAHPDVLEKAGASASDMLIAVTLHDEVNMVACQVANALFDIPTTIARIRAQT
                     YRHERWGRLFSKERMGIGQIISPELEVGDTVLRRLELPGAFDSAAFADNLVTAIGISC
                     GADCPIVDTPIRQLAELFPDLPAVIVGIIRKDQLIVPHGDDQISVGDDVYVIAQTDSV
                     GRVLKIFGHEEQRSRRVVIAGGGNIGLYVARQIEARGLNLKLKIIEASKARAVEIAEQ
                     LERTVVLHGSALSEELMREAEVGSADTIVTLTNDDQVNVLASALARQLGCGRSLTLIN
                     NQAYSGMARAFGIDAQINPRAITISRVLGQVRRGRIQQVHSVHNGAGEIIEAEALDTA
                     QAIGKPLKDIGISDGLRIGALIRKGRVLAPRGQTRLEAKDRVVLFARSSHVREVEQLF
                     RVSIEYF"
     assembly_gap    5692..5717
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          941 a         2027 c         1850 g          968 t
ORIGIN      
        1 ccgtgtggcg ctgcccattc ccggcggtga tgtgatcggc ggccgcccgc tcgaggcgat
       61 gacgctcgtc gccctcggat atcgctccct ttcgatggcg cccgcctcga tcggtccggt
      121 gaaagcgatg atccgatcgc tcgacgtcgg tgcggcccgc gaagtgctga catcgctgct
      181 caggaccgac gcgcgcagca tccgccgaga gattgcagac tttgccgcgg cacagggcgt
      241 caccatctga ggaggccgcc cggcatgatg cgcggcgcgt tcaccatcgg cggaattgag
      301 gtcagggccg gcgagcgccg cctcgtcgac ttgcctgtgg ccaagctctc gaaccacacg
      361 cccgtgacgc tccccgtgca cgtactgcat ggactgctgc ccgggccgac gctcttcatc
      421 tcggccgcga tccatggcga tgagctcaac ggcgtcgaaa tcatccgccg cgtactgcgc
      481 acactcatgc cgggcaacat ttctggaacg ctcctctgcg tccccgtcgt caatgccttc
      541 ggcttcattg gccattcgcg ctacctcccc gaccggcgcg atctcaaccg atcgttcccc
      601 gggtcggcga ccggatcgct cgccgcccgc ctcgccaacc ttttcctgaa cgaggttgtc
      661 cgccgctgca cggtcggcat cgatctgcac acggccgcag tacatcgcat caacctgccg
      721 cagatccgtg ccgacttcgt caagcggccc cgcacgcgcg agcttgccga agcctttggc
      781 gcccaggtcg tgctcgagag ccctgagcga tcgggatcgc tccgccgcgc cgcacgcgaa
      841 gccggcattg acgtgctcgt ctacgaaggc ggcgaggggc tacggttcga cgagttcgcc
      901 atcaaggccg gtgtcgacgg cattgccaac gtcatgctga agatcggcat gctgcaactc
      961 ccggatggcg tcgagccagc tgcgtccaat ccagcctatc gtccgcccgt gttcgcgaac
     1021 acgtcgcgct gggtgcgcgc gcccgacggc ggcgtgctcc gctccgtccg ccgcatcggc
     1081 gatgccgtcg gccaaggcga actcatcggc tatgtcgcca acccttacga ggaccgcgaa
     1141 ctcgaggtgc gcgcgccgca tcgtggcatc atcatcggcc gcaccacgct ccccgtcgtc
     1201 aacatgggag atgccctctt ccatatcgcc tggtcggccg aacacgatgc ctccgcgccg
     1261 aaggaagcgc tcatggacga ggacgagatc atctgaggtg gtcgacaatt tgcctgcgct
     1321 ttctctcatc ccatataacg acagagttta accttgcggg ccctgctcat gacccagacc
     1381 aacaatcgct ttctcgatga actcgccaag ctcatgacgg acgccgccgg tgccgcacag
     1441 ggtgcccgcc gcgaggtcga gacgctcatg cgcgcccagg gcgagcggat gttgcgcggc
     1501 atggacatcg tgcagcgcga ggagttcgag gccgtccgcg ccatggccgt ccgcgcccgc
     1561 gaggagaacg agcgcctcgc agagaaagtg gcccgcctcg aagccgaggt cgagcggctc
     1621 aagggcccaa tctgagtttc gctgcacgag cgaggcagac gcccgaggaa acaacaaggg
     1681 ccaggtccgg ggcggcccgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncagag
     1741 tgcggcccag tcgcgaagcg aacgcaatcg acggggcggc acacgctgtc aggttcgctt
     1801 cgcgtgcgag ggccagccct cgcgctcccg ccggaggaga tcctccggac ctcccctttc
     1861 tagttcggaa gatcgcgggc gacgcggaag ccgatcgagt agacgccgta atccggtttc
     1921 aagggctggc ggatcgaggc gcgcgcgtcg atcgccggaa agttccacga gccgccgcgc
     1981 atgacgacct cgccgcatgg cttgctgtcg attggcgggc cgccaatctc cggtaaccac
     2041 ctatgggtga gcgtcgggtt tcagcactcg gcgacgatct cccagacgtt gccgtgcatg
     2101 tcgaaaaggc cgaaggcgtt cgcggcatac gagccgacag ggacggctcg cccgcgactg
     2161 cgctccacgt tcgcgtcctt cgccgtcagt gtgtcgcccg tggaaaaacg cgtcgccgca
     2221 cccgcccggg ccgcatactc ccactcggct tccgtcggca gtcgatagga ttgccccgta
     2281 atggcggcga ggaacgtcag atattcgctg atggcgttcg ccgtcacgtt gaccaccggc
     2341 tgcctgccgc gcccccagcc ctcatccgcc ggccgaagcg tgcacatttt cgtcgcaacg
     2401 caggcatccc actcgtcgaa cgtcacctca tggcggccga gcgcgaacga cttcgtgaag
     2461 ctgattttcc gcgctggcaa ctccgatcgc tcggtctgtc ccgctgcctc caccgtcccc
     2521 atgacgaacg ttcccaccgg gatgaccgcc atctcggggc actcggtgca cgacgtgtga
     2581 tcgagccggt tgtcgcggaa gaatttgctg ggagcagaag tgccactcgg ggtaggcgca
     2641 gacgtgccag gactcggcgg cgcgctcggg tcggagcacg agcactcgcc gtcgagctcg
     2701 atatcgccgg aaggtctgag gacgacgaag agcttgcagc cgccgctcgg agagagtggc
     2761 gtgaggagtg tgccggtacg caccagcatg ttcttcccat cgactgcgat gccaccctcg
     2821 tagacctgct gtcccagatg cctggccgtc cgcgtcggaa cgatcacgat cccgcagccc
     2881 ggcaccgtaa atggctgccg cacgcgcacg acgctcttga cggaaacccg cgactcgcct
     2941 cgcgccggcg gcggcgcggg gggaacgatt cgtgaaggtt gacgcgcctc tggagttggc
     3001 ggcgtcccag gcggcgccgt ggcgataccc gagatcaccc ccactgcccg cgccttcacc
     3061 tccgcgcacg tggcactcgc cgccagcgcc tcgatctgcg cgcgtgcggc cgacccgcgc
     3121 ttggtcagcc gctcgagctc ttcgccctcg acgcggcacg ccgtcgccgc cgtctcgagc
     3181 tcccgcaacc gcacttcgag ggcggggagg caagggcatt ccgccccgca gttgcccaga
     3241 aattcgcgca cctctgctgc atcggacgac ttcggtaccg tcgcacatcg cacggcaagg
     3301 cgtgccgtcg cctccggatc gcggctcggc gtcgatgccg aaatgctcgc aagccgtgcc
     3361 gcaccatctc cgctcagcga cggcgtctga taacgccgcg gcgaggggaa cagccgcgtc
     3421 acgcgatggt cgatgcgccg cgcgaggaag gcgctgagtt cggctgccgt gacatgtccg
     3481 tcgcgatcaa ggtcagccgc gccgtagagc ccttcgacga gcgcgtccgt gaaaatgccg
     3541 agccgccgct cgcgatccca gttcgcctcc tggtcggagc cggacgctgc aagctcgacg
     3601 acccgcggct cggcagcccg gattgcactc ggcgatatgc ggacactact cgtctcggcc
     3661 gcgatccacc ccgcgccgga cgagcccgtg aaacaggcgt cgagatagag cacgacccgg
     3721 ccctccggcg aattcgcgcg ctgcgcgttc ccaagcgatt tccggatatc gnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnncg cttcgccggc atgccgtgcc ccgaatagaa gaaggtgatc
     3901 gtcgaagcgc gaccgagccg cgccttgagt cgctggagct ccggtccgcc ctccccgtcg
     3961 cgaccgaagt agccgaggaa gcccgcatac gtgaggttcc tctcgacgat gatccggtcg
     4021 gcgggcactc gcagcacctc gcgcgctgcc ttggcgaagg cctcgccgtc gcggtccgcg
     4081 tattcgactt tcggcaccgt gccgcgatag tcctggttcg cgatgatgat cacaatgtcg
     4141 tccggtccgg ccgcagctgg caggactgtc acgacgagtg ctgtcagcac gagcaccgcg
     4201 cgagccagaa ccgacatcac ccctcccgcc atcccctgtc gacatcaacg agcatgagag
     4261 cagaagcgct cgctcgcaag cccgctgaaa caatccggag acgttcgctc caccctcaga
     4321 aatactcgat gctcacgcgg aacaattgct cgacctcacg aacgtggctc gatctcgcga
     4381 acagcacgac gcgatccttg gcctcgaggc gcgtctgccc tcggggcgcg agcacccggc
     4441 ccttgcgaat gagcgcgcca atcctgagcc cgtccgatat gccgatgtcc ttgaggggtt
     4501 tgccgatcgc ctgggcggtg tcgagcgctt ccgcttcgat gatctcgcca gcgccgttgt
     4561 gcacggaatg cacctgttgg atgcgaccgc gcctgacctg gccaaggacc cgcgaaatgg
     4621 tgatcgcccg cgggttgatc tgcgcgtcga tgccgaacgc ccgcgccatg cccgagtagg
     4681 cctgattgtt gatcagcgtc aggctccgcc cgcagccgag ctgccgcgcg agcgcgctcg
     4741 caagcacgtt cacctgatca tcgttggtga gcgtgacaat ggtatccgcc gatccgacct
     4801 cggcctcgcg catcaattcc tccgagaggg cacttccgtg cagcacaacc gtccgctcga
     4861 gctgctcggc gatctcgacc gcccgcgcct tgctcgcctc gatgatcttg agcttcaggt
     4921 tgagccctct ggcctcgatc tgccgggcca cgtagaggcc gatgttgccg ccgcccgcga
     4981 tcacgacgcg gcgcgagcgc tgctcctcgt gaccgaaaat cttgagcacg cggccgaccg
     5041 aatccgtctg cgcaatcaca tagacgtcgt cgccgacgct gatctgatcg tcgccatgcg
     5101 gcacgatcag ctggtccttg cggatgatgc cgacgatcac cgcgggcagg tcggggaaaa
     5161 gctcggccag ctggcggatc ggcgtgtcga cgatcgggca gtcggcgccg cacgagatac
     5221 cgatcgcggt gacgagattg tcggcgaacg cggcgctgtc gaaggcaccc ggcagctcga
     5281 gccggcgcaa caccgtgtcg ccgacctcga gctcgggcga gatgatctgc ccgatgccca
     5341 tccgctcctt ggaaaagagc cgcccccaac gctcgtggcg gtaggtctgc gcgcgaattc
     5401 gcgcgatcgt cgtcgggata tcgaagagcg catttgcgac ctggcacgcg accatgttga
     5461 cctcgtcgtg caaggtcacg gcgatcagca tgtcgctggc gctggcgccg gccttctcga
     5521 gcacgtccgg gtgggcgccg ttgccgacgt aggcgcgcac atccagcgta tcgttggcac
     5581 gctgcacgag gtcgggccgc gtgtcgacga tggagatgtc gttgccctcg gccgccagac
     5641 gctcggcgat gccgaacccg acctggcctg ccccgcagat gatgaccttc annnnnnnnn
     5701 nnnnnnnnnn nnnnnnncgc ttggatggca cccatctagc gcgactttgg ccaccctgcc
     5761 taccgccgga gctgactcgc gcctgctact tggtcagcgt gtcggtcgcc ttcttggacg
     5821 gcgtgccggg atcgggcgtg gtcggggctg tcggcggcgg tgtcggccct ggggtcgggg
     5881 ctgccgtctg cggcggctgg cagcggaact ggaggcaggc gttgacgacg cgggt
//
LOCUS       DAORNH010000288         5894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10018_length_5894_cov_3.014557, whole genome shotgun sequence.
ACCESSION   DAORNH010000288 DAORNH010000000
VERSION     DAORNH010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10018_length_5894_cov_3.014557"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..2282)
                     /gene="fusA"
                     /locus_tag="PK264_21190"
     CDS             complement(186..2282)
                     /gene="fusA"
                     /locus_tag="PK264_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRD78420.1"
                     /translation="MPRTHKLEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVH
                     DGAATTDFMEQERERGITITSAATTCFWKGADGKERRLNIIDTPGHVDFTIEVERSLR
                     VLDGAVCVLDSNQGVEPQTETVWRQGDKYSVPRVIFSNKMDKTGADFYMCLADIKDKL
                     GARAVPLQLPIGAENNFKGVVDLLKMKAYVWDGDGKDAQFTEQEIPAELVDKAKEFRA
                     SMIWAAVEMDEAVAEKFMADEQEPDVATLKRLIRKATITRAFYPMLCGSAFKHKGVQQ
                     LLDAVVDFLPAPSDRQAYVGIDVRTGGEASRRPSDDEPLSMLAFKIMNFEHVGSLTFC
                     RIYSGKLEKGMALTQTTRDRKERAGMIYEMHADKRNEVKEAFAGDIVALAGLKDTRTG
                     ETLSDPSKPVLLEKMEFPEPVIEMKVEAKSKGDQEKMANVLHSLALEDPSFRVTVDPE
                     SGETRIKGMGELHLDIKVDIMRRAPHNVEVNVGAPQVAYRETLARSTDIDYTHKKQSG
                     GSGQFARVKFRIEPNEVGKGNEFESEIVGGSVPKEFIPGVEKGVKSVWDTGVLIGFPL
                     VDTKVTLTDGAFHEVDSSAIAFEIASRQGLREGCQKAGIKLLEPIMDVEVVTPGDFVG
                     GVIGDVNSRRGQIRNQEMRGNATVIRAYVPLANMFGYVSQLRSMSQGRASYTMQFAHY
                     AEVPRNVAEEVKAKYA"
     gene            complement(2312..2782)
                     /gene="rpsG"
                     /locus_tag="PK264_21195"
     CDS             complement(2312..2782)
                     /gene="rpsG"
                     /locus_tag="PK264_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRD78421.1"
                     /translation="MSRRRQADKREINPDPKFGDLVISKFMNSIMNQGKKSVAERIVY
                     GALERVEGKAKSDPVKLFHQALENVMPAVEVRSRRVGGATYQVPVEVRPERRQALAIR
                     WIISAARARNENTMSERLSGELLDAAANRGTAVKKREDTHKMADANRAFSHYRW"
     gene            complement(2888..3259)
                     /gene="rpsL"
                     /locus_tag="PK264_21200"
     CDS             complement(2888..3259)
                     /gene="rpsL"
                     /locus_tag="PK264_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRD78422.1"
                     /translation="MPTIQQLIRKPRHPKTYREKARHMEACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIPGEGHNLQEHSVVMIRGGRVKDLPGVRYHIIRGVL
                     DTQGVADRRQRRSKYGAKRPK"
     gene            complement(3645..4139)
                     /locus_tag="PK264_21205"
     CDS             complement(3645..4139)
                     /locus_tag="PK264_21205"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRD78423.1"
                     /translation="MFKTIVGFAAALVVMAAPAMAQRSSGTPSGLIDGSAQSAIQSIA
                     RSFGKVEMTKTRDGNPLIRAESGKFKYVVFFSDCRDGRCSNIQLIADFRRPDNFGERE
                     MNDWNKKRAFGKAFINDDGLAVISLNLLLSGGVSRANLDGQFDLWSKLLKEFDDFLYG
                     GGRR"
     gene            complement(4539..>5427)
                     /locus_tag="PK264_21210"
     CDS             complement(4539..>5427)
                     /locus_tag="PK264_21210"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD78424.1"
                     /translation="IELRAARWPATRAPRRGTVCVFPGRTEPIEKYFEVVADLRRRGF
                     AVAVLDWRGQGGSSRLLADPAKGHVRSFKDYDRDLAAFMREVVLPDCPPPYYALAHSM
                     GGNILLRTAARGASWFDRMVLVAPMLAINQARTPVNLSVARFGVEVLGTLGLAQAMIP
                     GGHRVPEEMRNGFEDNPFTTDRERFMRYRKVLEVSPSLGIGSPTLGWVRAAGRSMAWL
                     ARPDVVRAIQVPILFISAGDERIVSTPAVERLASRLKLAAYVTVPLARHEVLMERDEH
                     RARFWAAFDAYLGVTAAAA"
     assembly_gap    5428..5671
                     /estimated_length=244
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5681..>5894
                     /gene="ccmI"
                     /locus_tag="PK264_21215"
     CDS             5681..>5894
                     /gene="ccmI"
                     /locus_tag="PK264_21215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HRD78425.1"
                     /translation="MFLLWLSFACLAAAVVMALLRPLARDLGAGEPVSQVATDADVAV
                     YKDQLTEIEAEQRRGIIGAAEAEAARI"
BASE COUNT          979 a         1852 c         1752 g         1067 t
ORIGIN      
        1 atcgtcccca cgttcacgtg cggcttgttg cgatcgaatt tttccttgcc catggggctt
       61 ctcctctatc tttccggttg aaccgccagc ggcggccacg agcccgggtc atctctgatc
      121 attcgatttc accggtccgt ttgcggcgcc gccgccccgg acccctgacc cttcagatac
      181 aaggttcagg cgtatttcgc tttgacctct tcagcgacgt tgcgcggcac ctcggcgtag
      241 tgcgcgaact gcatcgtgta ggaggcgcgc ccctggctca tagagcgcag ctgactcacg
      301 taaccgaaca tgttggcgag tggcacgtag gcccggatga cagtcgcgtt gccgcgcatc
      361 tcctggttcc ggatctggcc gcggcggctg ttgacgtcgc cgatgacacc gccgacgaag
      421 tcgcccggcg tcaccacctc gacgtccatg atcggttcga gcagtttgat gcctgccttt
      481 tgacaacctt cgcgcaagcc ctggcgcgaa gcgatctcga aggcaatcgc ggagctgtcg
      541 acctcgtgga aggcgccgtc cgtcagcgtc accttggtgt cgacgagtgg gaagccgatc
      601 agcacgcccg tgtcccagac gctcttcacg cccttctcga cgccagggat gaactccttc
      661 ggcaccgagc cgccgacgat ctcgctctcg aattcgttgc ctttgccgac ctcattcggc
      721 tcgatccgga acttgacgcg ggcgaactgg cccgagccgc cagactgctt cttgtgcgtg
      781 tagtcgatgt ccgtcgagcg ggcgagcgtc tcgcggtagg caacctgcgg cgcgccgacg
      841 ttgacctcga cattgtgagg ggcgcggcgc atgatgtcga ccttgatgtc gagatgcagc
      901 tcgcccatgc ccttgatgcg agtctcgccg ctctccggat cgaccgtcac tcggaacgag
      961 ggatcttcca gcgccagaga atgcagcaca ttcgccatct tctcctgatc gcccttgctc
     1021 ttcgcctcga ccttcatctc gatgacgggc tcggggaact ccatcttctc gagcagcacc
     1081 ggcttcgagg gatctgagag cgtctcaccc gttcgcgtgt ccttgagacc ggcgagagcg
     1141 acgatgtcgc ctgcgaacgc ttccttgacc tcgttgcgct tgtcggcgtg catctcgtag
     1201 atcatgcccg cgcgctcttt tctgtcgcgg gtggtctggg tcagcgccat gcccttctcg
     1261 agcttgcccg agtagatgcg gcagaaggtg agcgagccga cgtgctcgaa attcatgatc
     1321 ttgaaggcga gcatcgaaag cggctcgtcg tcggagggac ggcgcgacgc ctcgcccccg
     1381 gtacgcacgt cgatccccac ataggcctgg cgatcggaag gcgccggcag gaagtcgacg
     1441 actgcgtcga gcaactgctg cacgcccttg tgcttgaagg ccgagccgca cagcatcggg
     1501 tagaaggcgc gcgtgatcgt cgccttgcga atgagccgct tcagcgtggc gacatccggc
     1561 tcctgctcat cagccataaa cttctcggcg accgcctcgt ccatctcgac ggcggcccat
     1621 atcatggagg cgcggaattc cttggccttg tcgacgagtt cggccgggat ctcctgctcg
     1681 gtgaactgag cgtccttgcc gtccccgtcc cagacgtacg ccttcatctt caggagatcg
     1741 acgacgcctt tgaagttatt ctcggcgccg atcggcagct ggagcggcac ggcacgggcg
     1801 ccaagcttgt ccttgatgtc ggcgaggcac atgtagaagt cggcgccggt cttatccatc
     1861 ttgttggaga agatgacgcg cggaaccgag tacttgtcgc cctggcgcca gacggtctcg
     1921 gtctgcggct cgacgccctg gttgctgtcg agcacacaca ccgcgccatc aagcacgcgc
     1981 agcgagcgct cgacctcgat cgtgaagtcg acgtgtcccg gcgtatcgat gatgttgagg
     2041 cggcgctcct tgccgtcagc gcccttccag aagcaggtcg tcgcggccga cgtgatggtg
     2101 atgccgcgct cgcgctcctg ctccatgaaa tcggtcgtgg ccgcaccatc gtgcacctcg
     2161 ccgatcttgt ggcttttgcc cgtgtaatag aggatccgct cggtcgtcgt cgtcttgccc
     2221 gcatcgatgt gggccatgat gccgaagttt cggtagtcct cgagcttgtg cgtgcgcggc
     2281 atggcgtgcc tcgaaacctg aaatctgttg cttaccagcg gtagtgcgag aaggcgcggt
     2341 tggcgtcggc catcttatgc gtgtcctcac gcttcttgac ggccgtgccg cggttagccg
     2401 cagcatcgag gagctcgccc gagagccgct cggacatcgt gttctcgttg cgggcgcggg
     2461 cggccgagat gatccagcgg atggcgagcg cctggcgccg ctcgggacga acctcgaccg
     2521 gaacctggta ggtggcgccg ccaacgcgcc gcgagcgcac ctcgacggcg ggcatcacgt
     2581 tctcgagcgc ctgatgaaaa agcttcaccg gatcgctctt cgccttgccc tcgacgcgct
     2641 cgagcgcgcc gtagacgatc cgctcggcaa ccgacttctt gccctggttc atgatcgagt
     2701 tcatgaactt ggaaatcacc agatcgccga acttgggatc cgggttgatc tcgcgcttgt
     2761 ctgcctggcg ccgacgggac attgtgatga tcctcgatag tctgaagggc ctgggcccaa
     2821 actctcccac cgcagtcgag ccggacggac ccggaccctg atgcggcacc gctcgcgcgt
     2881 gcgagcctta cttcggccgc ttggcgccgt acttcgaacg gcgctgacgg cggtcggcga
     2941 cgccctgggt gtcgagcacg ccgcggatga tgtgatagcg cacaccggga aggtccttca
     3001 cgcgaccgcc gcgaatcatg accaccgagt gctcctggag gttgtgaccc tcacccggga
     3061 tatagccgat gacctcgaag ccgttggtga gacgcacctt ggcgaccttg cgaagcgccg
     3121 agttcggctt cttcggcgtc gtcgtgtaga cgcgcgtgca cacaccgcgc ttctgagggc
     3181 acgcctccat gtggcgggcc ttctcgcggt acgtcttcgg atgccgcggt ttgcggatca
     3241 gctgctggat cgtcggcatt cgcccgaatt cccatcagag ccggtgatgc actggtgccg
     3301 aaccggcacc gtcacgcaca cggaccaaac ctaacctgcc gagccgctcc tgaggagcgc
     3361 ccggcgcatc aaccagagga tctcgcccga aggcgagatc atggcccaca ttttcctctg
     3421 cttatggacg gcagcgtcta gatcaggcga gcgaaaatcg cccgctgcat cgatctacgg
     3481 ccagccgtga gagtgggcgc aacctataca tgtcgacccg cgcggtcaac acgttttctc
     3541 gcaggttgtg gcccgatcgg acccgcttcg gcatcaggca acaaaaatgg tgccgaatca
     3601 atgattcggc accacccgaa caactgaact cagcgttccg ctgttcagcg acggccacca
     3661 ccatacagga agtcgtcgaa ctccttcagc agcttcgacc acagatcgaa ctggccgtcg
     3721 aggtttgcgc gcgagacgcc gcccgaaagc aagagattaa gagaaatcac ggcgaggcca
     3781 tcgtcgttga tgaaggcctt gccgaaggcg cgcttcttgt tccagtcgtt catctcgcgc
     3841 tcgccgaagt tgtcagggcg gcggaaatcg gcgatcagct ggatgttcga gcaccggcca
     3901 tcgcggcaat ccgagaagaa gacgacgtac ttgaatttgc ccgactcggc gcggatgagc
     3961 gggttgccgt cgcgcgtctt ggtcatctcg accttgccga agctgcgcgc aatggactgg
     4021 atggccgact gcgcactacc atcgatcagg cctgagggag taccgctcga gcgttgcgcc
     4081 atggccgggg cggccatcac caccagagcg gccgcgaagc cgacgatcgt cttgaacatt
     4141 gccgttaggt ccttcggttg ctgacggaag tactcaccag ttttcgaggc tgatccccga
     4201 tgacggtctt ccaagctcga gcgccgctcc ggcgaccgcg ctggtcgagg ttcggacggt
     4261 gtcgtgggaa gtgcaagcct gggagtgacg gcgcagcagc gccaccatgt cgcatcgggg
     4321 tctgatcgac gccgtcacca catccaccgg cccttgtact caccaccccc cacatagtcg
     4381 gctccggccc aatgacctga tcgaggaata gctctttcac agcgaaatac tctatcgcgt
     4441 ttgctagcgg atgaaaatgg ccagagcgct gcaagttcgt ttcgatcgtg tttcttcgtc
     4501 ggacggccgt cgacaccgtc cggacaccca tttcgggcct atgccgccgc agccgtgacg
     4561 ccgagatatg cgtcgaacgc cgcccagaat cgggcccgat gctcatcgcg ctccatcaga
     4621 acctcgtggc gcgcgagcgg caccgtgaca taggccgcaa gcttcaaccg ggacgcgaga
     4681 cgctcgactg ccggcgtcga gacgatgcgc tcatcgcccg ccgaaatgaa caggatcggc
     4741 acctggatcg cgcgcaccac gtccggccgg gcgagccaag ccatcgagcg gcccgccgca
     4801 cggacccagc cgagggtcgg cgagccgata ccgagggacg gcgacacctc caaaaccttg
     4861 cgatagcgca tgaaccgctc gcgatcggtc gtgaagggat tgtcctcgaa gccgttgcgc
     4921 atctcctctg gaacgcggtg cccgccggga atcatggctt gtgcgaggcc gagcgtgccg
     4981 agcacctcca caccgaaacg tgcgaccgac aggttgaccg gcgtccgcgc ctgattgatt
     5041 gccagcatcg gcgccaccag aaccattcga tcgaaccagc tcgcaccgcg agccgcggtc
     5101 ctgagcagga tattgccacc catcgaatgg gcgagcgcgt agtagggcgg cggacaatcc
     5161 ggcagcacga cctcgcgcat gaaggccgcg agatcccggt cgtagtcctt gaacgagcgg
     5221 acgtgcccct tggccggatc ggcgagcagg cgcgacgagc cgccctggcc gcgccagtcg
     5281 aggacggcga ccgcaaatcc gcgccggcgc aagtcggcca caacttcgaa gtacttctcg
     5341 atcggctcgg tccgcccggg gaacacgcaa accgtgccgc gccggggtgc ccgcgtcgcc
     5401 ggccagcgtg ctgcgcgcaa ctcgatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggagcatgt atgttccttc tctggctatc
     5701 gtttgcctgt cttgccgcag ccgtggtgat ggcgctcctg cgcccgctcg ccagggattt
     5761 gggcgcaggc gaacccgtct cccaagtcgc gaccgacgcg gacgtcgccg tctacaagga
     5821 ccagctcacc gagatcgagg ccgagcagcg tcggggtatc atcggtgcgg cggaagccga
     5881 agctgcacgt atcg
//
LOCUS       DAORNH010000289         5887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10031_length_5887_cov_3.526578, whole genome shotgun sequence.
ACCESSION   DAORNH010000289 DAORNH010000000
VERSION     DAORNH010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10031_length_5887_cov_3.526578"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..>900
                     /locus_tag="PK264_21220"
     CDS             297..>900
                     /locus_tag="PK264_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase"
                     /protein_id="HRD78426.1"
                     /translation="MTNPRPEQEIFDELAALTATPGFAHVIAAFCVRDNWIRSGGEMR
                     AENMAHLYSPERLIRTEMSTLIGLMVRAAPDFDAPPPDDAPALAERTEALLSELHDSM
                     MQPWSDTIREVIAGDKSSNPWTTGDRMREPIFYGGEGALSFQNCDFAARKYAADDEWL
                     ITNKGAAIADMIAVAKAIATVQTEKLTAAIRGKGREIGNAS"
     assembly_gap    901..919
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <920..2441
                     /locus_tag="PK264_21225"
     CDS             <920..2441
                     /locus_tag="PK264_21225"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRD78427.1"
                     /translation="TPTDLNPHLNLPRAKIIASVKALSFPNGNHNSCFKALCDFNAVN
                     AWPILTVDEDRFILFHWNALAEALYESPFYWLAADKSYFSAAFEHRGAFTEAFAFQRL
                     SDVFGAARAYQGVIIEEGKDRLGEIDVLVLFGDRAIVVQAKSKRLTLEARRGNDLRLR
                     GDFQAAVQDAYDQAMNCAEAFSNPSVRFVASDGTELAIPAKLTQIFPICLVADHYPAL
                     AFQADEFLVRRQAAAVLAPLIIDIFTLDAMAEMLDRPLRFLSYLELRALHGSKVHINH
                     ETALLSYHLKYNLWLDGDYDMVDVGDDFAADLEIAMSARRLVYPGKRTPDGILTLVAG
                     RKLDEIIAAIEAKPFGPMIDLVLLVFQLSGTAMKELADAVDWVLDCARQKGSSDFSIG
                     FAGSGLTVHANFSPLSEGKPCLERHMAFCKYRGKAESWYGLSLSPINGAIRVGIKAAF
                     PWTFDAEIDSLSKRLGKKATRPPALRSGTKLGRNDPCHCGSGKKYKNCHLAADTRR"
     gene            complement(2912..>3598)
                     /locus_tag="PK264_21230"
     CDS             complement(2912..>3598)
                     /locus_tag="PK264_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085938460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRD78428.1"
                     /translation="LPAARCPLPAARCPVERLMRSMGLQGVVRGKPVRTTISDKAVPC
                     PQDRVNRQFHAPAPNVLWLSDFTYVATWAGFVYVAFVIDAFARRIVGWRVSRTAHASF
                     VLDALEQALHERRPIHRGGLVHHSDRGSQYVSIKYTERLAKAGIEPSVGSVGDSYDNA
                     LAETINGLYKAEVIHRRGPWRSFEAVEFATLEWVDWFNNRRLLEPIGHIPPAEARFSA
                     AMAGAQVMAA"
     assembly_gap    3599..3698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3699..4255)
                     /locus_tag="PK264_21235"
                     /pseudo
     CDS             complement(3699..4255)
                     /locus_tag="PK264_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611958.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(3912..4028)
                     /locus_tag="PK264_21235"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(4371..5663)
                     /locus_tag="PK264_21240"
     CDS             complement(4371..5663)
                     /locus_tag="PK264_21240"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HRD78429.1"
                     /translation="MPASPEPGFAPPDGLRWLYVDFNSYFASVEQQLNPALRGRPVAV
                     IPVETDSTCAIAASYEAKAFGVKTGTPIYEARKMCPGLICVLARHEHYTAFHHRLIEE
                     IDRHIPVSAVCSIDEMACRLMKNERAPERAKAIALAIKEGIVRNVGAYVRCSIGLAPS
                     KYLAKVATDLQKPDGLTILMPEDLPERLYALALRDLPGIGRNMERRLNRAGIYDLRTL
                     MGLPPKHLRAVWGSLWGERMWYYLRGYDLPDPETDRRSVGHSHVLAPEMRSSDQAYII
                     ARRLTIKAAARLRRIGFCAERFSLSVRIEDGPRLSSEAYCTPARDSFLFLGLLEQLWQ
                     VMVRDSGGRRIKKIGVVLHGLVPKGDVHIQPDLFALPAATAATTRRREEISQAIDRLN
                     RKFGRDTILFGMTGGQEKFATSTKIAFTRIPDIEEFLE"
BASE COUNT         1054 a         1814 c         1784 g         1116 t
ORIGIN      
        1 acggcggtgc gcatgttccg cttggcgtct tcgatggtgc gcccgttgct gaagcacccg
       61 gggagcgagg gactccaggc gtagtagccc tcgtcctcgg cctccttctc gatgacgacc
      121 tcaaagttgt agaactgcac tgagtcaggg ctcctctgcg gccggacgcg ataaccgtgg
      181 tcctgcccgg gggcggaatg caagcgcatt tcacctctgg cgcgccgccg gcgctgccct
      241 gggtgcggat gtgctggccc cgcacgcgct tgcatatatg atgttaatat catgccatga
      301 cgaatccgcg gccagagcaa gagatcttcg atgaattggc ggcactcacc gccacacctg
      361 gctttgccca tgtgatcgcg gccttctgcg tgcgcgacaa ttggatccgg tctggcgggg
      421 aaatgcgcgc cgagaacatg gcgcatctct attcgccgga gcgcctcatc cgcaccgaga
      481 tgtctacgtt aattggcctc atggtccgcg cggctcccga cttcgatgcg ccgccgcctg
      541 acgatgcgcc ggcgttggcg gagcggacgg aggccctgct ctcagagctg catgacagca
      601 tgatgcagcc ttggtccgac acaattagag aagtgatagc tggtgataag tcgtccaatc
      661 cgtggactac aggcgatcgg atgcgcgagc cgattttcta cggcggcgag ggggcattgt
      721 cctttcagaa ttgcgatttc gccgcgagga aatatgccgc cgacgatgaa tggctcatca
      781 ccaacaaagg agccgcaatc gctgacatga tcgcggtcgc caaggcgatt gccaccgtgc
      841 aaaccgaaaa gctgacggcg gcgatccgtg gcaagggccg agagatcggc aatgcgagct
      901 nnnnnnnnnn nnnnnnnnnc acgccgactg atctaaatcc gcacctaaac ttgcctcgtg
      961 cgaagattat cgccagtgtg aaggcattat cttttccaaa tggcaatcac aattcgtgct
     1021 tcaaggcgtt atgcgacttc aatgccgtaa atgcatggcc cattcttact gtagatgaag
     1081 atcgtttcat cctcttccac tggaatgccc tcgccgaggc gctctatgaa tctccattct
     1141 actggctggc cgccgacaaa tcctatttct ctgctgcctt cgagcatcgc ggtgcgttca
     1201 ccgaggcgtt cgcgtttcag cgacttagcg acgtgtttgg ggcggcacgc gcctatcagg
     1261 gtgtcattat tgaggagggc aaggaccggc tcggcgagat cgacgtgctg gtgctcttcg
     1321 gggaccgtgc gatcgtcgtt caggcgaagt ccaagcggct aacgctggaa gcccggcgcg
     1381 ggaacgatct ccggctaagg ggcgacttcc aggcggccgt gcaggacgcc tatgaccaag
     1441 cgatgaactg tgccgaggca ttcagtaatc cgagtgtgcg gttcgtcgca tcagacggca
     1501 ccgaactcgc catcccggcc aagctgacgc agatcttccc aatttgcctg gttgcagatc
     1561 actatcccgc actcgcgttc caggccgacg agttcctcgt ccgccgccaa gcggcagccg
     1621 tgctggcacc gctcatcatc gacatattca ccctcgacgc gatggccgag atgcttgacc
     1681 ggccgctgcg cttcctgagt tatctcgaac tgcgggcgct acacggctcc aaggttcata
     1741 tcaaccatga gaccgcactc ctttcctacc atctgaaata caatctctgg ctggacggcg
     1801 actacgatat ggtcgacgtt ggggacgatt tcgcggccga cctcgagatc gccatgagcg
     1861 cgcgccgtct cgtctatccg ggcaagcgga cgccggacgg catcttgacc ttggtggcag
     1921 ggcgcaagct cgacgagatc atcgctgcga tcgaggccaa gccgttcgga ccgatgatcg
     1981 atcttgtgct gcttgtcttt cagctgagcg gcactgcgat gaaggaactc gccgatgccg
     2041 tcgattgggt actcgattgt gcccgacaaa aaggatcttc cgacttctca ataggatttg
     2101 ccggttccgg cctcaccgtc cacgccaatt tttcgcctct gagcgagggc aagccctgtc
     2161 tggagaggca catggcattc tgcaagtatc gcgggaaggc cgaaagctgg tatggcctca
     2221 gtctttctcc gatcaacggc gcgatccgcg ttggcatcaa agccgccttc ccttggacct
     2281 ttgacgccga gatcgatagc ctctccaagc ggcttgggaa aaaggccacg cgcccgccgg
     2341 ctttgcgctc tggcaccaag cttggccgca acgatccctg ccattgtggc agtggcaaga
     2401 aatacaagaa ctgtcatctg gccgccgaca cgcgccgtta agcaggcatc ccgaactcgg
     2461 agcacatttc aaggccacgt ccagtgatcg ccttcacctc caactgactt tctcggccga
     2521 attcggtccg gacggtgatc atcccccatg aaagcggtcg ccggtttggg cgacatggac
     2581 cctgtcctga tcgggatcga agacccccaa agtcgggtga aagcgccgac gactgcttta
     2641 cgtccgagat tgacaattct gacttctgca atcgacgatt tgaactgccc cggcttctcc
     2701 ggaggctgct ggggtcaaat tttgccgatc atgtgacgtg gtgttgacgg gcatttcttg
     2761 gtgagaccga gggaccattc ttcggcgccg acctgcactc cggggtgccg ggagtcgcag
     2821 gcggggtcaa ggagggtccg cgccgggtgc ggcgtggatg ccttgacacc gccgagcacc
     2881 cggcgatgct tgctgcaggc gggccgggtg ctcatgccgc catcacctgt gcgccggcca
     2941 tggcggcgga gaaccgggcc tcggcgggcg ggatgtggcc gatcggctcg agcaggcggc
     3001 ggttattgaa ccagtcgacc cattcgagcg tggcgaactc gacggcttcg aagctgcgcc
     3061 atggtccccg ccggtggatg acctcggcct tgtagagacc gttgatggtt tctgcgagcg
     3121 cgttgtcgta gctgtcgccg acactgccga ccgacggctc gatcccggcc ttggccaggc
     3181 gctcggtgta tttgatcgag acatactggc tgccacggtc gctgtggtgg acaaggccgc
     3241 cgcgatggat cggccgacgc tcgtgcagcg cctgttccag cgcgtcgagg acaaagctgg
     3301 catgggccgt ccggctgacc cgccagccga cgatccgccg ggcgaaggcg tcgatgacga
     3361 aggcgacata gacgaagccc gcccaggtgg ccacataggt gaaatcgctg agccagagca
     3421 cgttcggcgc cggagcgtgg aactgtcggt tgacgcgatc ctgcgggcac ggcaccgcct
     3481 tgtcgctgat cgtggtgcgc accggcttgc cgcgcacaac cccttgcaag cccatgctcc
     3541 gcatcagccg ctcgaccggg cagcgggcag cgggcagcgg gcagcgggca gcgggcagnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ggcagcgggc agcgggcagc
     3721 gggcagcggg cagcgggcag agcggacatc ccctcccggc cgagctgccg ccacaccttg
     3781 cggacgccat agacctcgaa gttctcggcg aagacccggg cgatctcggg tttcagcgcc
     3841 aagtcccgcc tggcccgccg ggacagcttg ccagggtccc gacgccgggc agcacaggcg
     3901 tgatacgggg acggggcgat cggcaacacc ctgcagatcg gctcgacccc gtatgcccca
     3961 cgatgatcgt cgatgaacgc gatcatggct tcgaccggcg gtcgagctcc gcctgggcaa
     4021 aataggcgga cgccttgcga agaatctcgt tcgcctgccg aagctcgcgg ttctcgcgct
     4081 ccagagcctt caaccgctcc gccacatcgg aaggaacgcc agctcgcttg ccgctgtcca
     4141 cggccgcctt ggtgagccag tcgttcagcg tgtgcggtgc gcacccgatc ttggctgcta
     4201 tcgagacaat cgctgcccag cgcgaggcat gctccccctg atgctccgtc accatccgaa
     4261 ccgcgcgctc gcggacctca ggcgaaaact tgttcgtcgg cttggaggct ccaccttctc
     4321 aggagttgga gcctccaaca atcccggggc ggttcagtgc ggcggcggcg ctattccaaa
     4381 aactcttcga tgtcgggaat gcgggtgaag gcgatcttgg tgctggtcgc gaatttctcc
     4441 tgtccgccgg tcattccgaa aaggatggta tcccggccga acttccggtt cagccggtcg
     4501 atggcctgcg atatctcctc gcgccgcctt gtcgtcgcgg ccgtcgctgc agggagagca
     4561 aacaggtctg gctggatatg cacgtcaccc ttcggcacca acccgtgcag cacgacgccg
     4621 attttcttga tcctcctgcc tccgctgtcc cgcaccatga cctgccagag ctgctccagc
     4681 agccccagaa acaggaaact gtcccgtgcg ggggtgcagt acgcttcgga agacaggcgc
     4741 gggccatcct cgatccggac cgacagcgaa aacctttccg cacaaaagcc gatgcgccgc
     4801 agccgcgccg cagcctttat cgtcagccgc cgggcgatga tgtaagcttg gtcggacgac
     4861 cgcatctcag gggcgaggac gtggctgtgc ccgacgctgc ggcggtcggt ctctggatcg
     4921 ggcaaatcgt agccgcgcag gtaataccac atccgctcgc cccagagact gccccacacg
     4981 gcgcgcaaat gtttgggcgg aaggcccatc agtgtccgca ggtcgtaaat gccggcacgg
     5041 ttcagacggc gctccatgtt tcgcccgatg cccggcaggt cgcgcagcgc cagcgcatag
     5101 agccgctccg gcaaatcctc cggcatcagg atcgtgagtc cgtccggctt ctgaagatcg
     5161 gtcgcaacct tggccaggta tttggacgga gcaagaccga tcgagcagcg cacatacgcc
     5221 ccgacattcc gcacgatccc ttccttgatc gcaagcgcga tggccttggc gcgttccggc
     5281 gcccgctcgt ttttcatcag gcggcaggcc atttcgtcga tggagcagac cgcgctcacc
     5341 ggaatgtggc ggtcgatctc ctcgatcagg cggtgatgga acgcggtgta atgctcgtgc
     5401 cgcgccagca cacagatcag tcccgggcac atcttgcggg cctcataaat cggcgtgccg
     5461 gtcttgaccc cgaacgcctt ggcttcatag cttgcggcga ttgcgcaggt gctatccgtc
     5521 tccaccggaa tgaccgcgac cggtcggccg cgcaaggccg ggtttagctg ctgctcgaca
     5581 ctggcgaaat agctgttgaa atcgacatac agccagcgca ggccgtcggg cggggcaaat
     5641 ccgggttcgg gcgacgcggg catgatgacc acgagagtta tgcgttatga gcttctttta
     5701 atccgatagt tcactatttg ttcttttctt tcacctgtca agatgcgctc gtgcaggggg
     5761 cgaaacccct gccgcgggtc caggcggcgc acgcctggtc gatgggaatg caaagggaga
     5821 accgacatct cccttcatct gtcctcttaa ccttcttgcc gttctatctt ggcgtgggag
     5881 gaaggca
//
LOCUS       DAORNH010000290         5848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10118_length_5848_cov_2.787330, whole genome shotgun sequence.
ACCESSION   DAORNH010000290 DAORNH010000000
VERSION     DAORNH010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10118_length_5848_cov_2.787330"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PK264_21245"
     CDS             <1..344
                     /locus_tag="PK264_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRD78430.1"
                     /translation="LTAARAYGLDIIDGVYNNFRDIDGLRRESLQARMLGFDGKSLIH
                     PDQVAIANEVFGPGDEEVASARKIIAAFAMPENRGKGVINMDGRMVELLHAEMAKRVV
                     VIADAIAARKK"
     gene            378..665
                     /locus_tag="PK264_21250"
     CDS             378..665
                     /locus_tag="PK264_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HRD78431.1"
                     /translation="MAKGYWIAHVSVTDPELYKQYVAANSVAFQKFGARFVVRGGRHE
                     TRRGTERQRHVVIEFDSFEIAKACHDSPEYAVAMAIRDRAAIVDLVIVEGF"
     gene            792..1550
                     /locus_tag="PK264_21255"
     CDS             792..1550
                     /locus_tag="PK264_21255"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRD78432.1"
                     /translation="MYVVQISDTHITTDGTHAAAREGALVRSVQELNRLDPPPLAVLH
                     TGDVAHTGRKGEYAGASRILAKLKAPLLIAVGNRDDREECRAAFPRVRELDRGGPFVQ
                     YAVDLGPVRVVALDTLSIDHGLGEACENRLVEASRLLAEVEPPTIVILHHPPVALPSL
                     YNASQFRNAEEADRLVRLIARQAGVLGVLAGHIHREDVVPIGATHLSTAPSLALDLRK
                     GRYTGAAGKQSFFHRHDIGERGLITSRLLVPPPV"
     gene            complement(1641..2615)
                     /locus_tag="PK264_21260"
     CDS             complement(1641..2615)
                     /locus_tag="PK264_21260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HRD78433.1"
                     /translation="MRAAVCSSLTGPDGITLETLPDPVPKEGEVVVRVKAAALNFLDT
                     LITRGRYQYKPEGAFSPAAEIAGVVESLGPGSTRLKPGDRVAGYIGWGGAREKVAVKE
                     SLLAPVPAGVDDNTAAAVSVTYGTAIHGLKDRALLKAGETVAVLGASGGAGLAAIEVA
                     KLLGARVIAVASTAEKLEVCRAGGADLLLNYAETDLKQGLRELTGGKGVDVVYDCVGG
                     EHTEAAIRSIAWEGRFLVVGFAAGQIPKVPINLMLLKNCALVGVFWGEMVTRHPDRQR
                     TNMDELMGWIASGRLRPHLHATLPLEKTAEGIRLIDARKVTGKVVITI"
     gene            complement(2766..3017)
                     /locus_tag="PK264_21265"
     CDS             complement(2766..3017)
                     /locus_tag="PK264_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78434.1"
                     /translation="MRAQNTEFELLTQAERAAGFSLVLDDTDDKPDTSTYSVDVPSSR
                     ALVPATTANAHGFAGRALSSANSVTTWIKGRVPMIGRPA"
     gene            complement(3390..>4405)
                     /locus_tag="PK264_21270"
     CDS             complement(3390..>4405)
                     /locus_tag="PK264_21270"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRD78435.1"
                     /translation="VLGGPNIYEARLAELMCARFPAVELVRFCNSGSEATLLCVSLAR
                     AITGRETVMVMNGAYHGSFLSFPSGKPAHLNAPFPIVTVDYNDPNAASAAIRSAGDRL
                     ACVILEPLMGSGGCIPATPEFLATLRRETEAVGAGLIFDEVMTSRLAPGGLHGALGLK
                     PDIVAFGKYLGGGLTFGAFGGRRDWMARLDPSRADVIVHSGTYNNNVLTMAAGIAGLE
                     TVFTPAAAARLNQSGDRLRARLNAAAEARGLPVRATGSGSMINIHPTARALARPADHA
                     HVPADARKLLHLEFNLAGQYVARRGFMALSLPLTDADHDALVTAFEAILDAHAGVLAA
                     LES"
     assembly_gap    4406..4464
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4465..4712)
                     /locus_tag="PK264_21275"
     CDS             complement(<4465..4712)
                     /locus_tag="PK264_21275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRD78436.1"
                     /translation="MAALRRNQSLADAVADAERRFIAANPKSRARAEAAARVMPGGNT
                     RTVQFYDPFPVAIVKGEAQRITDLDGHVYTDFLGEFTAG"
     assembly_gap    4752..4843
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4844..5517
                     /locus_tag="PK264_21280"
     CDS             <4844..5517
                     /locus_tag="PK264_21280"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRD78437.1"
                     /translation="ILLGLVFGLAMLPQMWVQQVIRRHAAPRGDLPGTGSEIARSLLD
                     RMGLAHVKVEMTKEGDHYDPMEKVVRLLPEHFAKSSLSAVVIAAHEVGHAMQDATGYA
                     PLIARTAIAKRAKRLETIGFAVLLLSPLVMLILKAPHVLIIELIGGLLLIGITVVMHA
                     VTLPVEFDASFKRALPLLGEARILGEKDMPAARQILRAAALTYVAAAAMSLLNIARWL
                     RVLRI"
BASE COUNT          967 a         1821 c         1909 g         1000 t
ORIGIN      
        1 gcctcaccgc agcgcgcgcc tacggcctcg acatcatcga cggcgtctac aacaacttcc
       61 gcgacatcga cggtctgcgg cgcgagtcgc tccaggcgcg tatgctcggc ttcgacggca
      121 agtcgctcat tcatcccgat caggtggcga tcgccaacga ggtcttcggg cccggcgacg
      181 aagaggtcgc aagtgcgcgg aaaatcatcg cagccttcgc catgccggaa aaccgcggca
      241 agggcgtgat aaacatggat gggcggatgg tcgagttgct gcacgccgag atggccaagc
      301 gcgtcgtggt catcgccgat gccatcgctg cgcgcaagaa atgagctgaa cgcggagcca
      361 agtggaggac agggccgatg gccaaaggat actggatcgc gcatgtttcg gtgactgatc
      421 ccgagctcta caagcagtac gtcgccgcca attccgtcgc gttccagaaa ttcggggcgc
      481 gctttgtcgt tcgtggcggt cgccatgaga cgcgccgggg cacggagcgc cagcgccacg
      541 tcgtcatcga gttcgacagc ttcgagatcg ccaaggcctg ccacgacagc ccagaatatg
      601 cggttgccat ggccatccgc gatcgggcgg cgatcgtcga tctggtgatc gtcgagggtt
      661 tctgagttgg tgcaccgccc gaggcttcgt cgacatcggc ggtagaccag attgcgcgac
      721 gcagctaaag atcgtcgcgg gccggcgcac cgtggggtgt cgatcggcaa gggacggtgt
      781 tccgcgcgga gatgtacgtc gttcagatct cggatacgca catcaccacg gacggcacgc
      841 acgcggccgc ccgagaaggt gcacttgtgc ggtcggtcca ggagctgaac cggctcgatc
      901 cgccgccgct cgccgtcctg catactggtg acgttgcgca tacggggcgc aagggcgagt
      961 acgccggcgc gagccgcatt ctcgccaagc tgaaagcgcc gctgctgatc gcggtcggca
     1021 atcgcgacga ccgggaggaa tgccgcgccg cgttcccgcg cgtgcgcgag ctcgaccggg
     1081 gcggcccatt tgtccagtat gccgtcgatc tcggaccggt tcgcgtggtg gcgcttgata
     1141 cactctcgat cgaccatgga ctgggcgagg cctgtgagaa ccgtctcgtc gaagcctcgc
     1201 gcctgctggc cgaggtcgag ccgccgacga tcgtgatcct gcatcacccg cccgtggcgc
     1261 tgccgtcgct ctacaacgcg agccagttcc gcaacgccga ggaagccgac cggctggtcc
     1321 gcctgatcgc gcggcaggcc ggagtgcttg gcgtgctcgc cggacacatc caccgcgaag
     1381 acgtcgtgcc gatcggggcg acgcacctgt cgacggcgcc gagtctggcc ttggatttgc
     1441 gcaagggccg ctatacgggc gcagcgggga agcaatcgtt cttccatcgt catgacatcg
     1501 gcgaacgcgg gctgatcacg agccggttgc tcgtaccgcc accggtatag ctcagacgtc
     1561 caagcatgct gcaggtcgag ggcgctgcgc ttgagttcgc tccaggcact tgtccctgga
     1621 cccaggcgga cgcccggcga tcagatcgtg atgacgacct tgccggtcac cttgcgggcg
     1681 tcgatgaggc gaataccttc ggcggtcttt tcgagtggca gggtggcgtg gagatgcggc
     1741 ctcaggcggc cgctggcgat ccagcccatc agctcgtcca tgttggtgcg ctggcggtcg
     1801 ggatggcgcg tcaccatctc gccccagaag acgccgacga gcgcgcagtt cttcagcagc
     1861 atgagattga tgggcacctt gggtatctgg cccgcggcaa agccgacaac gaggaaacgg
     1921 ccctcccagg caatcgagcg gatggccgcc tcggtgtgct cgccgcccac acaatcgtag
     1981 acgacgtcga cgcccttgcc gccggtgagt tcgcgcaggc cctgcttgag gtcggtctcg
     2041 gcgtaattga gcaggagatc ggctccccct gctcggcaga cctccagttt ctcggcagtt
     2101 gaggcaaccg caatcacgcg cgcgccgagc agctttgcga cctcgattgc ggcaagcccc
     2161 gcgccgccgg atgccccgag cacggccacg gtctctccgg ccttcaggag cgcgcgatcc
     2221 ttgagaccgt ggatcgcggt cccataggtc acgctgacgg cagccgcggt gttgtcgtcg
     2281 acgccagctg gaactggtgc aaggagcgat tccttcacgg cgactttctc gcgggcgccg
     2341 ccccagccga tgtagccggc gacgcggtcg ccgggcttca ggcgggtcga gcccggccct
     2401 aggctctcga ccacgccggc gatctcggcg gcgggtgaga atgcgccctc cggcttgtat
     2461 tgatagcgtc cacgcgtgat cagcgtgtcg aggaaattga gggccgcggc cttcacgcgc
     2521 acgaccactt cgccctcttt cggtaccgga tcggggagcg tctcgagcgt gatgccgtcg
     2581 gggccggtga gggacgagca cacggcggcg cgcatgagtg gctcctggtc aatacctggt
     2641 gtggatgagg aacggtcgca ctgttcgtgc ggcttgggcg ccctggcgtc aagggtgatg
     2701 gcgcacaaga agacggctgc cgctcaagcg acagccgccg acagcctcgt gacctattgc
     2761 aagtctcagg cgggtcgccc gatcatgggt acgcgtccct tgatccaggt ggtgaccgag
     2821 ttggctgagg agagggcgcg tccggcgaag ccatgggcgt tcgcggtcgt cgcagggacg
     2881 agcgcgcgcg atgagggcac gtcaaccgag tacgtggacg tatcaggctt atcgtcggtg
     2941 tcgtcgagga ccagtgagaa gcccgcggcg cgctccgcct gcgtgagcaa ctcgaattcg
     3001 gtgttctgag cccgcatggt atcacctccg cggcaacgcc gctgaaagat cgctactcgc
     3061 ttcgctgcga gtcgctcggc agcctcacct ctagcccgct cgcctacaac ccagatgaca
     3121 gtgtcttgga cccagaagct cggcccgagt gtctcctggc aggcgaaccg gctgcgaaag
     3181 cgtagaatgc cgtagttcta ttgatgcgac ataaacagcg catgtcaaga aacattggcg
     3241 agaattacac caatatttcg tcgctgcatt gcacttcttg cgatgcaaac gccaattttt
     3301 tctccatgaa cactatttgc ggagattccg cacgtcatgt gtcagcgagg cggcgaccac
     3361 cgcgctcacg gtggctgccg ggggcgcgat cacgattcga gagcggcaag cactcccgcg
     3421 tgagcgtcga gaattgcctc gaaggccgtg acgagcgcat cgtgatccgc atcggtgagt
     3481 ggcagcgaga gcgccatgaa cccccgtcgc gccacgtact gcccggcgag attgaactcg
     3541 agatggagga gcttgcgcgc atctgccggc acgtgtgcat ggtcggctgg cctcgcgagt
     3601 gctcgtgctg tcgggtggat attgatcatc gagcccgaac cggttgcccg gacgggcagg
     3661 ccgcgcgcct cggcggcggc gttgaggcgg gcgcggagcc gatcgccgct ttggttcagt
     3721 cgagccgctg cagccggcgt gaagacagtc tcgagaccag cgataccagc cgccatggtg
     3781 agcacattgt tgttgtaggt tcccgagtgg acgatgacgt ctgcgcgcga tggatcgagg
     3841 cgggccatcc agtcgcgccg cccgccgaag gcgccgaagg tgaggccgcc gccgagatac
     3901 ttgccgaagg cgacgatatc gggcttgagg ccgagtgcgc catggaggcc gccaggtgcc
     3961 aagcgcgacg tcatcacctc gtcgaagatc aggcctgcac cgacagcctc ggtctcgcgc
     4021 cggagcgtcg ccaggaactc gggcgtcgcc ggaatgcagc cgccggagcc catcaatggc
     4081 tcgaggatga cgcaggccag gcggtcgccg gccgagcgga ttgcggcgct ggcagcattc
     4141 gggtcgttgt agtcgacggt gacgatcgga aagggtgcat tgaggtgcgc gggcttacca
     4201 gaggggaacg agaggaagct gccatgatag gcgccgttca tcaccatgac ggtttcccga
     4261 cccgtaatgg cacgggcgag ggagacacag agcagcgtcg cctcgctccc ggaattgcag
     4321 aagcggacga gttcgactgc ggggaagcgc gcgcacatca gctcggcgag ccgcgcctcg
     4381 tagatgttgg gcccgccgag gacgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnncccgcc gtgaactcgc cgagaaagtc ggtgtagacg
     4501 tggccgtcga ggtcggtgat gcgctgcgcc tcacccttca cgatcgcgac cgggaagggg
     4561 tcgtaaaact ggacggtgcg cgtgttgccg ccgggcatca cgcgcgccgc cgcttccgca
     4621 cgcgcgcggc tcttggggtt ggcggcaatg aagcgccgct cggcgtcggc gactgcatcg
     4681 gcaaggctct ggttgcggcg tagggcggcc atggcgtcat cctgttgtcg aagtcagcgt
     4741 tcctctctat gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcatcct cctcgggctc
     4861 gtcttcggtc tcgccatgct gccgcagatg tgggtgcagc aggtgatccg gcggcacgcg
     4921 gcgccgcgcg gcgatctccc ggggacgggg agcgagatcg cccgctcgct tcttgatcgc
     4981 atggggctcg cccacgtcaa ggtcgagatg acgaaggagg gcgatcatta tgatcccatg
     5041 gaaaaggtcg tccgcctgtt gccggagcac ttcgccaagt cgtcgctctc cgccgtagtg
     5101 atcgccgcgc acgaggtcgg gcatgccatg caggatgcga ccggctatgc ccccctgatc
     5161 gcccggaccg cgatcgccaa gcgtgcgaag cggctcgaga cgatcggctt tgccgttctc
     5221 ctgctctcgc cgctggtgat gctgatcctg aaggcgccgc atgtcctgat catcgaactc
     5281 attggcggcc tgctgctgat cggcatcacc gtcgtcatgc atgccgtgac gctgccggtc
     5341 gagttcgacg cgagcttcaa acgcgcactg ccgctgctcg gtgaggcgcg tatcctcggc
     5401 gagaaggaca tgcccgcggc acgccagatc ctgcgggctg cagccctcac ctacgtcgcc
     5461 gcggccgcca tgagcctgct caacatcgcc cgctggcttc gcgtcttgcg gatatgattt
     5521 ccaccaccaa aatcttgatc tcagaacctc cgtcactacg gcttcacgtc aggagctgat
     5581 cgtccgacga ttttccggta gtgtccgtga gcgtttggga accgtagatt atctatcgca
     5641 ctcattccca cgcccttccc cgtcaaggcc gctcaagcgg cctcatgcgg ctcctcgatc
     5701 aacatgaacc gtacgactac ccactcccgc gcataggccc gcggcggcct ccctcagcag
     5761 cctgggatcg ccactggcgt gttgacgcgg gacactcgac gtccgatttc tggccagccc
     5821 gcggcggccg gcccgcctca ggcgtgtc
//
LOCUS       DAORNH010000291         5836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10146_length_5836_cov_2.891195, whole genome shotgun sequence.
ACCESSION   DAORNH010000291 DAORNH010000000
VERSION     DAORNH010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10146_length_5836_cov_2.891195"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PK264_21285"
     CDS             <1..289
                     /locus_tag="PK264_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78438.1"
                     /translation="AGATAGAIVALVVGAFVGVRWYGLVVPYSELVRILLATAVMVGA
                     LWFAPMKVGTFGLLAEVAAGAAIYGVAIMALMPRVLDRVIGLLRSGRRSAS"
     gene            complement(278..1456)
                     /locus_tag="PK264_21290"
     CDS             complement(278..1456)
                     /locus_tag="PK264_21290"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HRD78439.1"
                     /translation="MQSTTNIRPAVETHSDARDDAKFAPISRRQMIAGGGAALAAPLL
                     ISAQANEPPISLGALGQRAGVHFGSAIGDAVLDMPAARDLYLRQCRILTSDPGFIMPF
                     IRPEPERTTWGSTDRLVDFAAGHGVPLRAHCLIWNDALPDYMRRLSRSEVERLLDRHI
                     EEAMGRYRGRIESWDVVNEPINPYEPGPGHWRKGPWLTHLGETYVARALKRAAATDPS
                     ARLFINETWVQASDRAGYTARRRLIELIDSLRQSGVALHGIGLQCHMDAAKRFEPDDF
                     EAFLRELARHGVDIYITELDVDDLELDGDEASRDRHVAEAYGTLVSTVLRVPAVKAII
                     TWQLADQFTWFRNPWFTKSFPARRLRFPSRALPFDDKLRPKLAHKAIAETFLARALGR
                     "
     gene            1610..2377
                     /locus_tag="PK264_21295"
     CDS             1610..2377
                     /locus_tag="PK264_21295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRD78440.1"
                     /translation="MPLATIDGWGLNLASREEALHCLVRDSEAGDGYILACMNLDHLV
                     KLRSDEGLRSVYSNPSTYVMADGAPVAVLARRQSKLVERSPGPDLMIPLCRAAARRGL
                     PVYMFGTRMDVLERGADRLREACPGIDIRGIEAPPFGYDPRSAEADAAADRIAASGAK
                     IVLLGLGSPKQEIFAERALARHSHLGFLCIGAALDFHVGEQVRAPKVMRHLGLEWAWR
                     LATSPRRLAKRYFLCAALLADITVVRPVRRAITGAAG"
     gene            2407..3672
                     /locus_tag="PK264_21300"
     CDS             2407..3672
                     /locus_tag="PK264_21300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD78441.1"
                     /translation="MSAAPSKPYRVCIVYQMDPRGAKVGGIETHVRTLIERYPGDFEV
                     LLVGVDAVGDLKLGAPHRLEVGGRPMTFIPVLHYSDSKARDAARSLLQSLSLQFARGV
                     LRYTPAIRRIIGRDPVSFELQRNETAFLPALLRRPSVLIVHNEYKRDNKTDSLVSRYW
                     KIYRAGEAFAARRADRIICVNPAIKAQIGRDFPAHAGKAELMTVSVNTDIFRCQPQVT
                     GDGILRLAYAGRFEAQKDPPLMFRTIRCIADRIDGKVQFHYVGTSDPSSYPEFAAIDG
                     LTVRHGFQKPDGVARILGMCHATILTSNFEGLPVFLLESLASGRPMGAIRLPQFDPLI
                     IDSVSGFQVERRDTPEASAEALAERFVAMWAAIRDGRMRPEATAEVVKPYTVDAQLPR
                     LFAIHRSLQDARWKFSARTTPRIAASAGE"
     gene            complement(4051..4758)
                     /locus_tag="PK264_21305"
     CDS             complement(4051..4758)
                     /locus_tag="PK264_21305"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HRD78442.1"
                     /translation="MARKAFIALAVLLALAGFYVAWPAYTGHVIGQALKAGDAAALER
                     KIDFPRVRESLKSHLVVEVERKVAGLKEDGSGLRKLVAGRLTSERVRRTVASTVDTIV
                     TPTSVIRISQQGRTLKQAIERGLLEQLSGSLGTGDDGRKSLVGSILGRFSRRSADAAA
                     GAPPTSDDDDVTITATGRERRYGLANVKHVTPTGALSFEVGVNRDPGAPWPELLVELA
                     FTGFDWRVVRITPVLDR"
     gene            complement(4771..5286)
                     /locus_tag="PK264_21310"
     CDS             complement(4771..5286)
                     /locus_tag="PK264_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78443.1"
                     /translation="MRSTRFSAVATAVAVAATAGSMALAQLPKAKGTAAAKEAAVALN
                     SVEVRLFLEGRGEFSPDILPLKDFGTRNFETITSAFPADRFSSILIKVGLKTAGEAFL
                     KGRQVKVSLVYEKTKKVAWTRTISDVYIGSSGRVSRVLLAEDKACGPFKLIVTAGKAT
                     VEHTLHFSCGE"
     gene            complement(5312..5707)
                     /locus_tag="PK264_21315"
     CDS             complement(5312..5707)
                     /locus_tag="PK264_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HRD78444.1"
                     /translation="MLRRAILALTAVPAAIILITLAVTNKHPVKLILDPFRPDDPAAP
                     SITLPLFVYLIAMLIGGVMLGGLATWWNQGKWRRTARTKAQDSLRWQAEADRLTRERD
                     EHYASGRGGAADSAGSQGSRKSVALLGRR"
BASE COUNT          896 a         2009 c         1922 g         1009 t
ORIGIN      
        1 cgcaggcgcg acggcgggcg cgatcgtcgc gctcgttgtc ggtgccttcg tcggtgtccg
       61 ctggtacggc ctcgtcgtcc catactcgga gctcgtgcgc attctcctcg cgaccgccgt
      121 gatggtcgga gcgctctggt tcgccccgat gaaggtcggc acgttcggcc tgctcgctga
      181 ggttgccgcc ggagcggcga tctacggcgt cgcgatcatg gcgctgatgc cgcgcgtgct
      241 cgaccgcgtg atcggcctcc tgcgctcggg tcggcgctca gcgtcctagg gcacgcgcga
      301 ggaatgtctc ggcaatcgcc ttgtgggcga gcttcgggcg gagcttgtcg tcgaagggga
      361 gggcgcgcga ggggaaacgc aggcggcgcg ccggaaagct cttcgtgaac cacggattgc
      421 ggaaccaggt gaactgatcg gcgagctgcc aggtgatgat cgccttgacg gcggggacgc
      481 gcagcaccgt cgaaacgagc gtcccatagg cctcggcgac gtgccgatcg cgcgaagctt
      541 catcgccatc gagctcgagg tcgtcgacgt cgagctcggt gatgtagatg tcgaccccgt
      601 ggcgggccaa ttcgcgcagg aatgcctcga agtcgtcggg ttcgaagcgc ttggcggcat
      661 ccatgtggca ttgaaggccg atgccatgca gcgccacgcc ggactggcga agactgtcga
      721 tcaattcgat gagccggcgg cgcgcggtgt atcccgcccg atcgctcgcc tgcacccagg
      781 tctcattgat gaagagccgc gcactcgggt ctgtcgcggc ggcgcgcttc aaggcgcgcg
      841 cgacataggt ttcgccgagg tgggtcagcc acggcccctt gcgccagtgc ccggggcccg
      901 gctcgtaagg gttgatcggc tcgttgacga catcccagga ttcgatccga ccgcgatagc
      961 ggcccatcgc ctcctcgatg tggcggtcga ggagccgctc gacctcggac cgcgacagtc
     1021 ggcgcatgta gtcggggagc gcgtcgttcc agatgaggca atgggcgcgg agcggcacgc
     1081 catgccccgc cgcgaagtcg acgaggcggt ctgtcgagcc ccacgtcgtg cgctcgggct
     1141 cgggccggat gaacggcata atgaatcccg gatcggaggt caggatgcgg cactggcgga
     1201 gatagaggtc gcgcgcggcg ggcatgtcga ggacggcatc cccgattgcc gagccgaagt
     1261 gcacgccggc ccgttgtccc agggctccga gcgagatggg cggctcgttg gcttgtgcgg
     1321 atatgagcaa aggcgccgcc agcgccgcgc cccctccggc gatcatctgc cgccgcgaga
     1381 ttggcgcgaa ctttgcgtcg tcccttgcgt cactgtgggt ctcgacggcc ggccggatgt
     1441 tggtcgtact ctgcatgggc atccgcaact ttcggagcgc cgatgctcgc acgatccggc
     1501 ttccaagtcg taaaaaatcc gttaaagtgc gcaagctgtt tgtgcagcgc aaaaacacca
     1561 gtcagagcgt agcgttatgg gttcgtcctc acgtcaccac acaattccga tgccgctcgc
     1621 caccattgac ggctggggcc tcaatctggc ctcgcgcgag gaagcgctgc actgcctcgt
     1681 ccgcgacagc gaggccggtg acggctacat cctcgcctgc atgaacctcg accatctcgt
     1741 caagctgcgc tccgacgagg gcctgcgctc ggtctacagc aatccgagca cgtatgtgat
     1801 ggccgatggc gcgccggtcg ccgtgctggc ccgccggcaa agcaagctcg tcgagcgctc
     1861 gcccggcccc gacctcatga tcccattgtg ccgggccgcc gcacggcgtg gcctgccggt
     1921 ctacatgttc ggcacccgca tggacgtgct cgagcgtggc gccgatcgcc tgcgcgaggc
     1981 gtgcccgggc atcgatatcc gtgggatcga ggcgccgccg ttcggctacg atccgcgttc
     2041 ggccgaagcg gacgcagcag ctgaccgtat cgcggcgtcc ggtgccaaga ttgtactcct
     2101 cggcctcggc tcgccgaagc aggagatctt tgccgagcgc gcgctcgccc gccattccca
     2161 cctcgggttc ctgtgcatcg gcgccgcgct cgacttccac gtcggcgagc aggtgagggc
     2221 gcccaaggtg atgcgccatc tcggcctcga gtgggcctgg cggcttgcga cgagcccgcg
     2281 ccgtctcgcc aagcgctatt tcctctgcgc ggcactgctg gcggacatca ccgttgtgcg
     2341 accggtgcgt cgcgcgatca cgggtgccgc gggctgagac gtcattcgca agcgaggtct
     2401 gttccgatgt cggctgctcc gagcaagcct tatcgcgtct gcatcgtcta ccagatggac
     2461 ccgcgcggcg cgaaggttgg cgggatcgag acgcacgtgc gcacgctcat cgagcgctat
     2521 ccgggcgatt tcgaggtcct gctcgtcggc gtcgacgcgg tgggcgacct gaagctcggc
     2581 gccccacacc gcctcgaggt cggcggtcgg ccgatgacct tcatccccgt tctgcactat
     2641 tccgacagca aggcgcggga cgctgcgcga agcctcctcc agtcgctgtc gctgcaattc
     2701 gcgcgcggcg tgctccgcta tacgccggcc atccgtcgca tcatcggccg cgatccggtc
     2761 tccttcgaac ttcagcgcaa cgagacggct ttcctcccgg cgcttctccg ccgcccgagc
     2821 gtgctcatcg tccataacga gtacaagcgc gacaacaaga ccgacagcct cgtctcccgc
     2881 tactggaaaa tctatcgcgc gggcgaagcc ttcgccgcgc ggcgggccga tcgcatcatc
     2941 tgcgtcaatc ccgccatcaa ggcccagatc ggacgggatt tcccggcgca cgccggcaag
     3001 gcagagttga tgacggtctc ggtcaacacc gacatcttcc gctgccagcc ccaggtgacg
     3061 ggagacggca tcctccgcct cgcctatgca ggccggttcg aggcgcagaa ggatccgccg
     3121 ctgatgttcc gcaccatccg gtgcattgcg gaccggatcg atggcaaagt ccagttccac
     3181 tacgtcggca cgagtgatcc gtcgagctac cccgagttcg cagcgatcga cggcctgacg
     3241 gttcgccacg gcttccagaa gcccgacggc gtcgcacgca tcctcggcat gtgccacgca
     3301 acaattctca cctccaactt cgagggactg ccggtcttcc tgctcgagtc gctcgcctcg
     3361 ggccggccga tgggcgccat ccggctccca cagttcgacc cgctgatcat cgacagcgtc
     3421 agcggctttc aggtcgagcg gcgcgacacg cccgaggcct ctgctgaggc gctggccgag
     3481 cgattcgtcg ccatgtgggc tgccattcgc gacggccgca tgcggcccga ggcaaccgcc
     3541 gaggtcgtga agccctacac ggtcgacgcc cagctgccgc gcctcttcgc gatccaccgc
     3601 agcctgcagg atgcacgctg gaagttcagc gcgcgcacca ccccgcgaat tgccgcctcc
     3661 gccggcgagt gaaccgcctg agcacacgtt cgctcgatgt ctttgctttt gggattattc
     3721 tggctcctga ccgtcttgtg gcggccatac tggtggttcg ttacgtcact gatcggtcca
     3781 ctgattacgg ccggaaagtg ggccaaacgc atccgctctc tagcctgtgc cgccgacatc
     3841 cgcgctgccg catgtccgca ccagatcaaa ttcagttctg aataggacag cccctccgac
     3901 atcgccgccg gtcacggaaa caccgagcac gcgtttccgc cgtccatgac cgttgggtgc
     3961 gcgaaggata cccatggcgc ctccgggcga ggattacgtg ccgcagcgcc ggctcaaggt
     4021 ctcccgccgc cacccgccca gccgcatcca tcagcgatcg agcacgggcg taatgcgcac
     4081 gacccgccag tcgaagccgg tgaaggccag ctcgacgagc agctccggcc agggcgcgcc
     4141 cggatcgcga ttgacgccga cctcgaagct cagcgcgccg gtcggggtga catgcttcac
     4201 gttggcgagg ccgtagcggc gctcgcggcc cgtggccgtg atggttacgt cgtcgtcgtc
     4261 cgaggtgggt ggtgcgcccg ccgcggcatc ggccgagcgc cggctgaagc ggccgagaat
     4321 gctgccgacg aggctctttc gcccatcatc gccggtgccg aggctgcctg acagctgttc
     4381 gaggaggcca cgctcgatcg cctgcttcaa ggtgcggccc tgctgcgaga tgcggatcac
     4441 cgacgtcggc gtgacgatgg tgtcgacggt cgaagcgacc gtacggcgca cgcgctcgga
     4501 cgtgaggcgt ccggccacca gcttgcgcag gcccgagccg tcctccttga ggccggcgac
     4561 cttgcgctcg acctcgacga cgagatggct cttcaggctt tcccgcacgc gcggaaagtc
     4621 gatcttgcgc tcgagggccg cggcgtcgcc ggccttgaga gcctggccga tcacatgccc
     4681 cgtgtaggcc ggccacgcga catagaagcc tgcgagagcc agcagcacgg ccaaggcgat
     4741 gaacgctttc ctcgccatcg cgtggtggcc tcactcgccg cagctgaaat gcagagtgtg
     4801 ctcgaccgtc gccttgcctg cggtcacgat cagcttgaac gggccgcagg ccttgtcctc
     4861 ggcaagcagg acccggctca cccggccact cgatccaatg tagacgtcgc tgatggtccg
     4921 ggtccaggcg accttcttcg tcttctcgta gacgagcgag accttgacct gccgaccctt
     4981 caggaaggcc tcgcccgccg tcttcaggcc gaccttgatg aggatgctcg agaagcggtc
     5041 cgcgggaaag gcgcttgtga tcgtctcgaa gttgcgggtg ccgaaatcct tgagcggcag
     5101 gatgtcgggc gagaactctc cccggccctc gaggaagagc ctcacctcga cgctattcag
     5161 cgcgacggct gcctccttgg cggcggccgt gcccttggcc ttcggcaact gggccagcgc
     5221 catcgatcct gccgtggcgg caacggcgac cgccgtcgcg actgcgctga agcgcgtgct
     5281 tctcacgtgc atcccctcac ctgtgtcacc gctatcgccg cccgagaagg gcgacggact
     5341 tgcgcgaacc ctggctgccg gcactgtccg ctgcgccgcc ccgcccgctc gcatagtgct
     5401 cgtcacgctc gcgcgtcagc cggtcggcct cagcctgcca gcgcagcgaa tcttgcgcct
     5461 tggtgcgtgc ggtccgccgc cacttcccct ggttccacca ggtggcgagg ccgccgagca
     5521 tcacgccgcc gatcagcatg gcgatgaggt agacgaacag cggcagcgtg atcgacggcg
     5581 ccgccggatc gtccggccgg aatggatcga ggatgagttt caccgggtgc ttgttggtca
     5641 ccgcgagcgt gataaggatg atcgccgcgg gcaccgccgt cagcgccagg attgcccgtc
     5701 tcagcaaggt cgaaactccg tcttgacgct gtcggatcgt accggcgcgt tcgctcaggc
     5761 cttggcgcgc gcgggcgcgg gagccttctc gcgggccgcg ccgtcagccg tgcgggcggc
     5821 cttcgccccc ttcgcg
//
LOCUS       DAORNH010000292         5807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10216_length_5807_cov_2.865264, whole genome shotgun sequence.
ACCESSION   DAORNH010000292 DAORNH010000000
VERSION     DAORNH010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10216_length_5807_cov_2.865264"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK264_21320"
     CDS             complement(<1..307)
                     /locus_tag="PK264_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRD78445.1"
                     /translation="MDKMLIDASHPEETRVVVLRDGRVEEFDFESANRRPLRGNIYLA
                     KITRVEPSLQAAFVEYGGNRHGFLAFGEIHPDYYQIPVADRQRLIEEEQRAAAEAEQR
                     "
     gene            1166..2407
                     /locus_tag="PK264_21325"
     CDS             1166..2407
                     /locus_tag="PK264_21325"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD78446.1"
                     /translation="MAALLGVLALSALVHLPGAAQSPPAPGARPPAPVVKAARPKVAA
                     KAVARGAEVRNEDGRTRFLLSANAPVAFSTYTLDAPYRVVIDIADLEFALPAASGQSG
                     GGLIKSYRFGLLAPGRARVILDIGGPMRVEAAQLMSGGTQGLHYLVVDIVGVEEGAFQ
                     RSDPPSLPAAAPAPAKPAKPSTKPVVMIDPGHGGIDSGTQGTAVLEKDIVLQVSLLLR
                     RYLLASGAVDVHMTRTGDTFVSLDERLRRSSELESSLFISIHADSIGAIEFAQSVRGA
                     TVYTLSERASDEAARALAEKENSADARVGIDAGAAADKDQVLGILFDLMRRDTAEHAA
                     EFQSGVIANLGRQVALAKDPRRSAAFKVLKQPQSPSVLIELGYMSNAEDEALMRTADW
                     QKKVAQAIAKAVTDYFARRAK"
     gene            complement(2426..2785)
                     /locus_tag="PK264_21330"
     CDS             complement(2426..2785)
                     /locus_tag="PK264_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD78447.1"
                     /translation="MQRILLAEDDDSMRTFLERALVKAGYEVTAFGNGVEAFQQLQSE
                     PFTLLLTDIVMPRMDGIELARRASEIAPEMKIMFITGFAAVTLNTETQPPKDARVLSK
                     PFHLKDLVREVDRLLAA"
     gene            2990..3889
                     /locus_tag="PK264_21335"
     CDS             2990..3889
                     /locus_tag="PK264_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HRD78448.1"
                     /translation="MPANERASILAELTPPFAVYRPVRQTTPFVFCSPHSGRIYPRAL
                     REASRLDALTLRKSEDCYVDLLFDWVPEIGAPLLVAHFPRAYLDVNRDEGELDPELFI
                     ERVPVNAQTQSLRVSGGLGVIARIVADSEEIYRERFPLAVATERIERLYRPFHSALDA
                     LLRDTVARFGYAVLIDCHSMPSASTGPHGPARPDIVLGDRFGAAADARLVRLARDSLI
                     ALGYDVQLNRPYAGGYITEKYGRPSTGLHALQIELNRGLYLDETNLTPVTGFSELRGY
                     LRSFAVKLIESTEAHFGRRQAAE"
     gene            complement(3905..4159)
                     /locus_tag="PK264_21340"
     CDS             complement(3905..4159)
                     /locus_tag="PK264_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78449.1"
                     /translation="MRVAVIAITVLVGMLVQGCANERSNPLSLSAAAAEAPTGAITRT
                     PQPPPVEASRKIASSRFLAAMALERATGLKPDPGRLVEQP"
     gene            complement(4275..>5807)
                     /locus_tag="PK264_21345"
     CDS             complement(4275..>5807)
                     /locus_tag="PK264_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRD78450.1"
                     /translation="DEEPVDYLHPMLEGILKETYGVIIYQEQVIQIAQVMGGYTLGQA
                     DLLRRAMGKKDKDEMARQKARFVEGADKNGVKKSDAEYIFELVDKFAGYGFNKSHAAA
                     YALVSYHTAYLKANYREEFLAASMTLDMANTDKLAMFAAEARRSGITVLPPCVNASGV
                     DFGAAPPGKDAPIDQRSGQPRGAIRYSLAALKNIGESAVAAIVEARETGGPFTTLGDF
                     GKRLNPKAFNKRGLETLARSGAFDALEPDRARVAGNVDAIMALAQRTAADSEAGTTDL
                     FAGGSSSGLDLRPAKGWTPMEKLQEEFAGIGFYLSGHPLDEYQALLPKLGVQRFAEFE
                     QRIERGAAAGRLAAIVVSARERRSQKGSKFAFAQFSDATGQFEAIVFSDTLAACRDLL
                     EPGTPVLVSVEAERDGETTKMRVQGIEALDKAVAGLQRGYAIVLDRRAVQAGTALAEL
                     KQLLKPGGKGEIRLMVEIDDFPSRGSGRAVEFTMPGRYDLSPHVRGLLTTAPGVLDVR
                     DL"
BASE COUNT          914 a         1901 c         1983 g         1009 t
ORIGIN      
        1 ggcgctgctc ggcttctgca gccgcgcgct gttcttcctc gatcaggcgc tgccggtcgg
       61 cgaccggaat ttggtagtag tccggatgga tttcgccgaa ggcaaggaag ccgtggcgat
      121 tgccgccgta ctcgacgaag gcggcctgca gagacggttc gacccgcgta atcttggcga
      181 ggtagatgtt gccgcggagc gggcggcggt tggccgattc gaaatcgaac tcctctacgc
      241 gtccatcccg gagcacgacc acccgcgttt cctcggggtg gctcgcgtca atgagcatct
      301 tgtccattgg ctcttgtcct tgtcgcggca tggtgcaccg tccacatgcc cggacaggcg
      361 gccggacgta cgccggtcgg tggacatgcc gcgaaatgtt gtgactggtc gacccttggc
      421 gcggccccgt cgtgcacgga cgacgcctct accgggccca gacgagaggg cgcgcgggtc
      481 gggagcggct ccggacagat cggtctgctg tcgggtcatg atcctgtgct cgggccgagg
      541 cccatggctg cgaccgacga ccgaaggggc ggcggtctag gtcaggggag gctcgcgccc
      601 ccaggcggtg cgggctgccg actgagctca gccggcggct gcgttaggtt ctatggccgg
      661 gaccgcgcag ccctgatcgc ccgaaagacc atccgcgcaa ggcgcgtccc ggtcgaatag
      721 gcgatccacg agctggctcc agggcgcaga tgtcccaccc agcctgggct gggcgcgacg
      781 tgtgcggagc gtgaccgctc ttgcgccacg gagccggtat cgggacccgg aatgctcgcg
      841 ctggtctggc ctgctcggag cccgctcgaa gatctcgggg aggcgcgcgg gttaccttgc
      901 ggtgttgctg gcgcgcgctc atcccgtcgt gatcggggcc cgggctcgat cgcgccaggc
      961 acgattgtca acacatacgg tctcgctatc ctgatggcaa gcagaagggc gaccttgcga
     1021 ccaggttgcc gacacgcggc ggcgggacta gaggattgtg cccgacaaag tcacaagcaa
     1081 ccgttaacgg gtgtcgcggc gcgggccagt ccgtcgccgg agcgtcatgc ggaggttcga
     1141 cgtggggagc agggcgaaag ccatcgtggc ggctcttctc ggcgtgctcg cgctgagcgc
     1201 gctggtacat ctcccgggtg ccgcccagag cccgccggcg ccgggcgcgc gaccgccagc
     1261 accggtcgtc aaggcagcgc ggccgaaagt tgcggcaaag gctgtggcgc ggggggcgga
     1321 ggtgcggaat gaggacggac ggacgcgctt cttgttgtcg gccaatgcgc cggttgcctt
     1381 ctcgacctac acgctcgacg cgccctatcg ggtcgtgatc gatatcgccg atctcgagtt
     1441 cgcgctgccg gcggcgagcg gccagagcgg tggcggactg atcaagagct accgtttcgg
     1501 gctattggcg ccgggccggg cccgcgtgat tctcgatatc ggcggcccga tgcgagtgga
     1561 ggctgcacaa ctcatgtcgg gcggcacgca gggtctccac tacctcgtgg tcgacatcgt
     1621 cggtgtcgag gagggggcgt tccagcgaag cgacccgccg agcctgccgg ccgcggcgcc
     1681 agcgccggcc aagcccgcca agccgtcgac aaagcccgtc gttatgatcg atccgggcca
     1741 cggcgggatc gattccggca cccagggcac ggccgtcctc gagaaggaca tcgtgctgca
     1801 ggtgtccctc ctcttgcggc gctacctgct ggcgagcggg gctgtcgacg tccacatgac
     1861 gcggaccggg gacacgttcg tctcgctgga tgagcggctt agacgatcga gcgagctcga
     1921 gtcgagccta ttcatctcga tccacgccga ttccatcggg gcgatcgagt ttgcccagag
     1981 cgtgcgcggg gcaaccgtct acacactctc ggagcgggcc tccgacgagg cagcgcgggc
     2041 gcttgccgag aaggagaatt ccgccgatgc ccgcgtcggg attgatgccg gtgctgctgc
     2101 ggacaaggat caggtgctcg gcatcctgtt cgatctcatg cgccgggaca cggccgagca
     2161 cgcggcggaa ttccaatcgg gcgtcatcgc caatctcggc cggcaggtgg cgctggcaaa
     2221 agatccgcga cgctcggcgg cgttcaaggt gttgaagcag ccgcagtcgc cgtccgtgct
     2281 gatcgagctc ggctacatga gcaacgccga ggacgaggcg ctgatgcgga ccgccgactg
     2341 gcagaaaaag gtggcacagg cgatcgccaa ggcggtcacg gactacttcg cgcgccgggc
     2401 gaagtagcgg ctgttcgggc cccggtcagg ccgcgagcag ccggtcgacc tcgcgcacca
     2461 gatccttcag gtggaagggc ttcgagagga cgcgcgcatc cttcggcggc tgcgtctcgg
     2521 tattcagcgt cacggcagca aacccggtga tgaacatgat cttcatctcg ggggcgatct
     2581 cgcttgcgcg ccgggcgagc tcgatgccgt ccatgcgggg catcacgatg tcggtgagga
     2641 gcagcgtgaa tggctcgctc tgcagctgct gaaacgcttc gaccccgttg ccgaacgcgg
     2701 tcacctcgta gccggccttc acgagcgcgc gctcgagaaa ggtgcgcatg gaatcgtcgt
     2761 cctcggccag aaggatgcgc tgcatgggac gtctcgcgag ttctgcggtc atggctctgc
     2821 ccgttcacat cttgtgccgg gtccgtcatg gagggagaat ctgtcgcgat tttggtaaac
     2881 agtcgctaaa cccgtgtcgt ccccatgaaa cgattgccgt cctgcgtcgc cgcatggaca
     2941 gcgtgccgcc atgatgtcta cataggctcg cgcgaaagca gggtggtcaa tgccagcgaa
     3001 cgaacgagcc tcgatccttg cggagctgac gccgccgttc gctgtctatc gcccggtgcg
     3061 gcagacgacg cctttcgttt tctgctcgcc gcactccggg cgcatctatc cgcgggcgct
     3121 tcgcgaggcg tcgcgcctcg acgcactcac cctgcgcaag tccgaggact gttacgtcga
     3181 tctactgttc gactgggtcc cggagatcgg cgcgccattg ctcgtcgcgc atttcccgcg
     3241 ggcctatctc gacgtcaatc gtgacgaggg cgagcttgat cccgagctct tcatagagcg
     3301 ggtaccggtc aatgcgcaga cgcagtctct gcgcgtctcg ggtggcctcg gcgtgatcgc
     3361 gcggattgtc gcggacagcg aagagatcta tcgcgagagg ttcccgctcg ccgtcgccac
     3421 cgagcgcatc gagcgcctct atcgaccttt ccattcagcg ctcgacgcgc tgttgcgcga
     3481 taccgtcgcc cgcttcggct acgcggtgct gatcgactgc cattcgatgc cgtcggcatc
     3541 caccggcccg cacggccccg cccgtcccga catcgtgctc ggtgaccgct ttggtgccgc
     3601 ggccgatgca cgcctcgtca ggctggcgcg ggacagtctg atcgcgctcg gctacgatgt
     3661 gcagctgaac cggccttacg ctggcggcta catcactgaa aagtatggca ggccctcgac
     3721 cggtcttcac gcgctgcaga tcgaactcaa tcgcggcctc tatctggacg agaccaacct
     3781 gacgcccgtg accgggttca gcgagctccg cgggtatctg cgctcattcg ccgtgaaact
     3841 catcgaaagc accgaagcgc acttcgggcg gcggcaggct gctgagtagc gggagccccc
     3901 gactctacgg ctgctcgacc agacgacccg ggtccggctt caggccggtt gcacgctcga
     3961 gcgccatggc ggcgaggaaa cgggacgagg cgatctttcg agatgcctcg acggggggcg
     4021 gctgcggagt gcgtgtgatc gcgcccgtcg gtgcctccgc ggcggccgcc gaaagcgaca
     4081 gcggattgct ccgctcgttg gcgcagccct gaacgagcat cccgaccaga acagtgattg
     4141 cgatgaccgc aacgcgcaaa gcctacccca ctccctgccg gattttgacc cgaccttgac
     4201 cgcaatatag tcgacgagcg gcatcgcttc aatcgcttgt ccgcagtttt cgcacctctg
     4261 cgtgcagcca ggcgtcacag atcccggacg tcgagtacac ccggcgccgt cgtcaacaac
     4321 ccgcgcacgt gcggcgagag gtcgtagcgg ccgggcatcg tgaactcgac ggcgcggccg
     4381 ctgccgcgac tcgggaaatc atcgatctcg accatcaggc ggatctcacc cttgccgccg
     4441 ggcttgagta gctgcttcag ttcagcaagc gcggtgccgg cctgaacggc gcggcggtcg
     4501 agcacgatcg cgtagccgcg ttgcagcccg gcgaccgcct tgtcgagggc ctcgatgccc
     4561 tggacccgca tcttggtcgt ctcgccgtcg cgctcggcct cgaccgaaac gagcacgggt
     4621 gtgcccggct cgaggaggtc gcggcaggcg gcgagggtgt cagagaacac gatcgcctcg
     4681 aactggccgg tcgcgtccga gaactgggcg aaggcgaact tcgagccctt ctgcgaacgc
     4741 cgctcgcgcg ccgacacgac gatcgccgcc agcctccccg cggccgcgcc gcgctcgata
     4801 cgctgctcga actcggcgaa gcgctgcacg ccgagcttcg gcagcagcgc ctgatattcg
     4861 tcgagcgggt ggcccgagag atagaagccg ataccggcaa actcctcctg cagcttctcc
     4921 atcggcgtcc agcccttcgc cgggcggaga tcgaggccgc tgctggagcc gcccgcaaag
     4981 agatcggtcg tgcccgcctc gctgtccgcg gccgtgcgct gggcgagcgc catgatggca
     5041 tcgacgttgc cggcgacccg cgcacgatcc ggctcgagcg cgtcgaaggc gcctgacctg
     5101 gcgagcgtct cgaggccgcg cttgttgaag gccttcggat tgaggcgctt cccgaaatcg
     5161 ccgagcgtcg tgaaggggcc gcccgtctcg cgcgcctcga cgatcgctgc aaccgcgctt
     5221 tcgccgatgt ttttcagtgc ggcgagggag tagcggatgg cgccgcgcgg ctggcccgag
     5281 cgctggtcga tcggcgcgtc cttgccgggg ggcgcagcac caaagtcgac gcctgaagcg
     5341 ttgacgcagg gcggcagcac ggtgatgccc gagcggcgcg cctcggcggc gaacatggcg
     5401 agcttgtccg tgtttgccat gtcgagcgtc atggaggcgg cgaggaactc ctcgcggtag
     5461 ttcgccttga ggtaggcggt gtgatagctg acgagagcat aggccgccgc gtggctcttg
     5521 ttgaagccat agcccgcgaa cttgtcgacg agctcgaaga tgtattcggc atcgctcttc
     5581 ttgacgccgt tcttgtcggc gccctcgacg aagcgcgcct tttgccgcgc catctcgtcc
     5641 ttgtccttct tgcccatggc gcggcggagg agatcggcct gcccgagcgt gtagccgccc
     5701 atcacctgcg cgatctggat cacctgctcc tggtagatga tgacgccgta ggtctccttg
     5761 agaatgccct cgagcatcgg atgcagatag tcgaccggct cctcgtc
//
LOCUS       DAORNH010000293         5772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10278_length_5772_cov_2.374497, whole genome shotgun sequence.
ACCESSION   DAORNH010000293 DAORNH010000000
VERSION     DAORNH010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_5772_cov_2.374497"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PK264_21350"
     CDS             <1..203
                     /locus_tag="PK264_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78451.1"
                     /translation="ADPLIAAECDEPDPDWSYLPDRDVRRAELARNYRINHDRYQAMK
                     GRRGICRASLRAQFPAPRPSKE"
     gene            237..578
                     /locus_tag="PK264_21355"
     CDS             237..578
                     /locus_tag="PK264_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78452.1"
                     /translation="MMHRSTFETPDLGLMLGRLLERSEATLERLDRIDSRLEAGDTRM
                     DEITSRIAAIEAIEERREEAQDAPKRPVLPIAETVIKAAAPYLIALLTLWLTGSAESA
                     LKLLAAASSGK"
     gene            complement(894..1364)
                     /locus_tag="PK264_21360"
     CDS             complement(894..1364)
                     /locus_tag="PK264_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HRD78453.1"
                     /translation="MTICKWLSIKLAASLIAIVQPWPAQAEPVEKSRIEVVDGDSVRL
                     DGIEWRLKGFDTPEIATSRCEAERRLGLLAKARLEALITAATVVDLAGDGEKDRHKRP
                     LGDLLLDGVNVRDLMVTELLARPYNGGRRKGWCSFDSREDLVPGLPPSRGKGKG"
     gene            1795..>2671
                     /locus_tag="PK264_21365"
     CDS             1795..>2671
                     /locus_tag="PK264_21365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD78454.1"
                     /translation="MSLLALALGLATVGRSYASDPAASTPTPVPALPVVPVKAAGDSR
                     LDGTEQRTRFIIGLERKVDFHVSSLPNPNRVVVDLPNVQVQLPQLPHGQAVGLVKSFR
                     GGLSAPGRMRLVIEVAAPVIVEKATVETVDNRARLVLEIIPADPRSAPKKALTTSGLA
                     KLGAGAPPATADSAPISAAVPAAVQPPTPKPAKRPEARAATAYKPIIVLDPGHGGHDS
                     GAIRHGAVEKDVVLAFSLVLKSRLEATGRYKVLMTRSTDKFIPLHERREFGAGRDMGE
                     AESEEVIAQGVLMHRI"
     assembly_gap    2672..2759
                     /estimated_length=88
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2760..3101
                     /locus_tag="PK264_21370"
     CDS             <2760..3101
                     /locus_tag="PK264_21370"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRD78455.1"
                     /translation="KLKGVGDTGTVSNILADLSARATLATRERTGTFVRSVIEFMGQS
                     TSMKDNPDRSAAFAVLRTAKVPAVLIELAYVSNAQDAVNLKSDAWRNKVSTSIVTAVE
                     NYFSHQVARLP"
     gene            complement(3132..4268)
                     /locus_tag="PK264_21375"
     CDS             complement(3132..4268)
                     /locus_tag="PK264_21375"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78456.1"
                     /translation="MTGCADLYLTSEHRQLRDTVRAFAAAEMAPLVREAEESERFPRQ
                     LFRRWGDLGLIGARYPAEDGGVGMDKVADCIVREELSYVCQAFASSLSAHAHLGIWPI
                     WRIGTPAQKARYFAPGVAGEKIGCFGLSEPDVGSNVRGLKTRAEKVQGGYRITGSKLY
                     ITNSPIADFMLLAARTGAAELSLFIVDLPNPAISISRLAKEGIRASETGLIAIDNAFV
                     PDEALLGGKTGTYPVILGSLSENRVGVAANAVGIARAALDASLAFARERRIGEKRVAD
                     FQAIRHKLADMAAEIDAARVFVMHGARFVDEGRLEPDMASKVKLVASEMAVRVTESAI
                     RIHGGAGIMRDYPVGRYHRDALVYIIGEGTAEIQRNIIARGLGI"
     gene            complement(4294..4926)
                     /locus_tag="PK264_21380"
     CDS             complement(4294..4926)
                     /locus_tag="PK264_21380"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD78457.1"
                     /translation="MTPVTWTIDLISPFPYLSLPRLPEIERRRPIRVRPVLFGALLQH
                     FKHLGPAEIPLKRLHTYRLAAFTAATQGQPFRFPPVHPFNPLPMMRILTALDGDMAAV
                     RRALALVWGEGRDPTSPETLMAVAGASSPADLEALIERTGAKEKLRTATAAASVEGIF
                     GVPTLTIDGEHFWGADALGMAIAFLDDPALFRSGELKRAGEVPVGVERKR"
     gene            complement(4966..>5772)
                     /locus_tag="PK264_21385"
     CDS             complement(4966..>5772)
                     /locus_tag="PK264_21385"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HRD78458.1"
                     /translation="NHGRAVAWGARRAGCRAVIYIHEGVSEGRAEAIRALGADVRREG
                     HHYDESVRLCATAAAANGWQVVSDTSWPGYEDVPRMVMQGYALLAAEAYAQGARPTHI
                     FLQGGVGGLAAAVASWFWEELGPARPKIIVVEPDKAACLLASAELGRATHVNGDLATI
                     MAGLACGEASPLAFKILHGGADAFMSIPDEAAATMMRRLAEDGVVGGESGVAGLAGLA
                     AAAVDEQARSLLGLDATSTILLIGSEGATDPDVYRSIVGRSADEVAATTA"
BASE COUNT          937 a         1946 c         1886 g          915 t
ORIGIN      
        1 cggccgaccc tctgatcgcg gccgagtgcg atgagcccga tcccgattgg tcctacctcc
       61 cagatcggga cgtgcgccgc gccgagctcg cacggaatta tcggatcaat catgaccgat
      121 accaagccat gaagggccgc cgcggcattt gccgcgcgag cctcagggcg cagttcccgg
      181 caccgcgccc gagcaaggag tgaccatgct cgcccgcaat gattggacgc tccgcgatga
      241 tgcatcggtc aactttcgag acgccggacc tcggtctcat gctcgggcgc ctgctcgagc
      301 gctcggaggc gaccctggag aggctcgaca ggatcgacag ccgcctcgag gcgggcgaca
      361 cgcgcatgga cgagataacc tcacgaattg ccgcgatcga ggccatagag gagcgccgcg
      421 aggaggcaca ggatgcgccg aagcggccgg tcttgccgat cgccgagacg gtgataaagg
      481 cggcggcccc gtacctgatt gcgctgctca cgctctggct cacggggtcg gcagagtcgg
      541 cgctgaaact gctcgcggcc gcttccagcg gcaagtgaac gcggcggcac ggcgcctgtg
      601 gtcgctcgag acgaagtacg acaggagaat ctggcgcgcg acgctggcgg ccgtggccta
      661 catggctttc gtcgtcgtgc tgagggccat tgcgtgaacg cgtccttctg gctgctctac
      721 ctcgcagcga tcctcgcctg gtggctgagc gtcacgcctt cctgagaccc gtcaggccgc
      781 ccggcggcca ttccgcatcg accaggcagc gcgcgtccag cccggtcgaa agcccccgca
      841 ccgcttgccc tcggcggtgc gggggccttt ttcgcgttgg cgagcgtagg ttcctagccc
      901 ttgcccttgc cacgcgaggg cggcaggcct gggacgagat cctcgcggct gtcgaacgag
      961 caccagccct tccggcgtcc tccattgtaa ggtcgcgcaa gaagctcggt gaccatcaag
     1021 tcacgaacgt tgacgccgtc gaggagcaga tcgccgagtg gccgcttgtg acggtccttt
     1081 tcaccgtcgc cggctaggtc gacgacggtg gcggccgtga tgagcgcctc gagtctcgct
     1141 ttggcgagga gcccgaggcg ccgctcggcc tcgcagcgag acgtcgcgat ttccggcgtg
     1201 tcgaagccct tcaatcgcca ctcgatgcca tcgagacgga cgctgtcgcc gtccacgacc
     1261 tcgatgcgtg acttctcgac cggctcggcc tgggccggcc acggctggac gatcgcaatc
     1321 aggctcgcgg cgagcttgat cgaaagccac ttgcagattg tcatgggcgc gctcctccgg
     1381 cgagtggtag agcactccag aagtgggaac aaggccagcg tccgagcccg tagcgcagcg
     1441 cccgattttc cattcccacc gctcgtagaa cattgaaatt tttcgacaag ccaagtccct
     1501 cctggggcac catgctcggc gacgctgcga tctgcagcga cggcactcga acgcgatcca
     1561 gaccctgcca cgtgcgttga cgccgatcag tgcgcagcgc gatcggcatg cttaacggtc
     1621 tcttaaccag cgttgtggat aacgaaagct gaacgccatc cgcaaattgc catcattgtt
     1681 cggcactcga tgtgtcaccg ttcggaccgt gaagcgtaga cgggggtcta tagtatgagt
     1741 tcccgccggt tggcccggcg aagcgatcgc ttcgcgtcgg ccgagtacat tgctttgtcg
     1801 ctgctcgcac tcgcgctcgg gcttgcgact gtcgggcgct cctatgcgtc cgaccccgct
     1861 gcgtcgacgc cgaccccggt tccggctttg cccgttgtgc cggtgaaggc cgccggcgac
     1921 tcccgactcg acggcaccga gcagcgcacg cgcttcatca ttggtctcga gcgcaaggtc
     1981 gactttcacg tctcctcgct gcccaatccc aatcgcgtcg tcgtcgacct gccgaacgta
     2041 caggtgcagc tgccgcagct gccgcacggc caggccgtcg gactggtcaa atcgttccgc
     2101 ggcgggctct ccgctcccgg ccgcatgcgc ctcgtcatcg aggtcgcggc ccccgtgatc
     2161 gtggagaagg cgacggtcga gacggtcgac aacagggccc ggctcgtcct cgagatcatt
     2221 ccggccgacc cgcgcagcgc gccgaagaaa gcgctcacga catcgggtct cgccaagctc
     2281 ggcgcaggcg cgccgcccgc gacagcggat agcgcaccca tatccgcagc ggtgccggcc
     2341 gccgtccagc cgccgacacc gaagcccgcc aaacgccccg aggcccgcgc ggccacggcc
     2401 tacaagccga tcatcgtgct cgatcccggc cacggtggtc acgacagcgg tgccatccgt
     2461 cacggcgcgg tcgagaagga tgtcgtgctc gccttcagcc tcgtcctcaa gtcgcggctc
     2521 gaggcgacgg ggcgctacaa ggttctcatg acccgcagca cggacaagtt cattccgctg
     2581 cacgagcggc gcgagttcgg cgctggtcga gacatgggtg aagccgagag cgaggaggtg
     2641 atcgcgcagg gcgtgctcat gcaccggatt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
     2761 agctgaaggg cgtcggcgac accggaaccg tctcgaacat cctcgccgat ctttcggccc
     2821 gagccacgct tgcaacgcgc gagcgcaccg gcacgttcgt gcgctccgtc atcgagttca
     2881 tgggtcagag caccagcatg aaggacaacc ccgatcgttc agcggcgttc gccgtgctgc
     2941 gcaccgccaa ggtgcctgcg gtgctgatcg agctcgccta cgtgtcgaat gcgcaggacg
     3001 ccgtcaacct caagtcggac gcctggcgga acaaggtctc gacctcgatc gtgacagctg
     3061 tcgagaacta tttcagccac caggtcgccc ggttgccctg agggccgccg agcagctgtc
     3121 gggcgccacc gtcagatccc gaggccacgc gcgatgatgt tgcgctggat ctcggccgtc
     3181 ccttcgccga tgatatagac gagcgcatcg cggtggtagc ggcccaccgg gtagtcgcgc
     3241 atgatgccgg cgccgccgtg gatgcggatc gcgctctcgg tgacgcgaac agccatctcg
     3301 ctcgcgacga gcttcacctt ggaggccatg tcgggctcga gccggccttc gtcgacaaac
     3361 cgtgcgccat gcatgacgaa cacgcgcgcg gcgtcgatct cggctgccat gtcggcaagc
     3421 ttgtggcgga tcgcctggaa gtcggcgacg cgcttctcgc cgatgcgccg ctcgcgggcg
     3481 aaggcgagcg aggcatcgag tgcggcccgc gcgataccga cggcattggc cgcgacgccc
     3541 acccggttct cgctaagcga accgaggatc acaggatagg tgccggtctt cccaccgagc
     3601 agcgcttcgt ccgggacgaa ggcgttgtcg atcgcgatga gcccggtctc cgaggcgcga
     3661 atgccctcct tggcgaggcg cgaaatcgag atcgcggggt tcggcagatc gacgatgaag
     3721 aggctgagct ccgctgcgcc cgtgcgcgcg gcgagcagca tgaagtcggc gatcggcgaa
     3781 ttggtgatgt agagcttcga gccggtgatg cgatagccgc cctggacctt ctcggcccgc
     3841 gtcttgagcc cgcgcacgtt cgagccgacg tcgggctcgc tcagaccgaa gcagccgatc
     3901 ttctcgcccg cgaccccggg agcgaagtag cgcgctttct gggccggcgt gccgatgcgc
     3961 cagatcggcc agatgccgag atgggcgtgt gcagacaggc tcgaggcgaa cgcctggcag
     4021 acgtagctca gctcctcgcg gacgatgcag tcggcgacct tgtccatgcc gacgccgccg
     4081 tcctccgccg gatagcgcgc tccgatcagg ccgaggtcgc cccagcggcg gaacagctga
     4141 cggggaaagc gctcgctctc ctccgcctcg cggacgagcg gtgccatctc ggcggccgca
     4201 aacgcacgca ccgtgtcgcg cagctggcgg tgctcgctcg taaggtacag atccgcgcaa
     4261 ccagtcatgc ctcgacgccc cctcagccga cgttcatcgc ttgcgctcga cgccgaccgg
     4321 gacctcgccg gcccgcttca gctcgcccga tcggaagagc gccgggtcat cgaggaatgc
     4381 gatcgccatg ccgagtgcgt ctgcgcccca gaagtgctcc ccgtcgatgg tgagcgtcgg
     4441 cacaccgaag atgccttcga ctgaggcggc ggcggtcgca gtccgcagct tttccttggc
     4501 tccggtccgc tcgatgagcg cctcgaggtc agcaggtgat gaagctccgg caacggccat
     4561 caaggtctcg ggcgacgtcg gatcacgccc ctcaccccac acgagcgcga gcgcgcgccg
     4621 cacggcggcc atgtcgccgt ctagcgcggt caggatgcgc atcatgggga gcggattgaa
     4681 gggatgcacg gggggaaagc ggaacggctg gccctgcgtc gctgccgtga acgcggcaag
     4741 ccggtaggtg tgcagccgct tcagcggaat ttcggcgggt cccagatgct tgaagtgctg
     4801 gagcagcgcg ccgaacagta ccggcctcac ccggatcggt cggcggcgtt cgatctccgg
     4861 caggcgcggc agcgaaagat agggaaacgg cgagatgaga tcgatggtcc aggtgaccgg
     4921 tgtcattttg cgaccttcat ggatgattgt gtctgtccga tcggctcaag ccgttgtggc
     4981 agcgacctcg tccgccgacc ggccgacgat cgagcggtag acatcggggt ccgtcgcgcc
     5041 ctcgctgccg atcaggagga tcgtcgacgt cgcgtcgagg ccaagcaagg agcgcgcctg
     5101 ctcgtcgact gcggcggctg caaggcccgc gagcccggca acgccggatt ccccaccaac
     5161 gactccgtcc tcggcaagcc ggcgcatcat agtcgccgcc gcctcgtcgg ggatcgacat
     5221 gaaggcgtcc gcgccgccgt gcaggatctt gaaggccagc ggcgaagctt cgccgcaggc
     5281 gagacccgcc atgatggtcg ctagatcgcc attgacgtgt gtcgcccgac cgagctccgc
     5341 gctcgcgagc aggcacgcgg ccttgtcagg ctcgacgacg atgatcttcg gccgcgccgg
     5401 gccgagctct tcccagaacc aggaggcgac ggccgcggcc agccctccga cgccgccttg
     5461 caggaaaatg tgcgtcggcc gggcgccctg ggcgtaggcc tcggccgcca gaagcgcgta
     5521 gccctgcatg accatgcgcg gcacgtcctc atagccgggc caactcgtat ccgagacgac
     5581 ctgccatccg ttcgccgccg cggcagtcgc acagaggcgc acgctctcgt cgtagtgatg
     5641 cccttcgcga cgcacatctg cgccgagcgc ccggattgcc tcggcgcggc cttcgctgac
     5701 gccctcgtgg atgtagatga cggcgcggca gccggcgcgg cgcgcgcccc acgcaaccgc
     5761 gcggccatgg tt
//
LOCUS       DAORNH010000294         5766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10292_length_5766_cov_3.119594, whole genome shotgun sequence.
ACCESSION   DAORNH010000294 DAORNH010000000
VERSION     DAORNH010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10292_length_5766_cov_3.119594"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PK264_21390"
     CDS             <1..922
                     /locus_tag="PK264_21390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD78459.1"
                     /translation="GQVLARLGPGEQAARVAKARVTIEVAEVNIKRAEANLAKARAVL
                     DQRQETNRRKQTLAGRDIVSRQAAEEAARDEAIAIADVAIAESEISSTKAQLADARAT
                     LAFEEAMLGHRTLVAPYDAIVIERHKELGTVVKAGDPVFTLVALDGYWGLAFVDEARA
                     GAIHEGQPVVARLRSLPRDAFHGKVLRIGLESDRVTEERRVYVRGDNPPPRVFLGEQV
                     EFLITVTSLERALLVPEAAVHGYNGREGRVWTLESGRLVRRLVQFRHRTEDGRLEIVE
                     GVPDGVLVVTRSDPGWREGQAARASEVKPK"
     gene            919..2052
                     /locus_tag="PK264_21395"
     CDS             919..2052
                     /locus_tag="PK264_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78460.1"
                     /translation="MNLAYRDVRHNLGRFLLTCVGLGLLLGVVMAMIGIYRGLIVDAL
                     TIARAPAVDLWVVESGSRGPFAEASRIPGDVREAVARIAGVAEAGSITYQTIEAEHRG
                     SKLRLYVIGYEPSRPGAPPEIVDGRGIARSHFEIVADRTSGLTLGDRITLGRNVFTVV
                     GLTRQQVSSGGDPAAYITLADAQRLQFDLAPPAVRVEIARGATAGSRDIVNAVIARLQ
                     PNASPDAVAETIRRWRHLSAITQEGQEQILTQSLVDRARRQIGLFTSLLLVVSAVIIA
                     LIIYTMTMEKLKQIATLKLIGAPDRTIIAMIVQQSLALGSIGFAAGAALIFALKDRFP
                     RRVVLEPDNVVTLGFIVLAVCIVASGLGVRAASKVDPATALGG"
     gene            2054..2770
                     /locus_tag="PK264_21400"
     CDS             2054..2770
                     /locus_tag="PK264_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78461.1"
                     /translation="MGSKQGALVDILRVSKHFGDGDARVDALREVSISVHPGEVVALL
                     GPSGSGKTTLLNVIGCILDPSAGSMQLDGERVLENGRWLRSDLRRLRLDKIGFIFQFH
                     NLIPFLDATENVALVCELAGDDAISARAKAVELLDYLEVSHRRSAMPAKLSGGEAQRV
                     AIARALANSPRIILADEPTAALDSKRAGIVMDLLRKLARERSAAIIAVTHDEKIFDRF
                     DRLHVLRDGMLVETRQGAGP"
     gene            complement(2778..3320)
                     /locus_tag="PK264_21405"
     CDS             complement(2778..3320)
                     /locus_tag="PK264_21405"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRD78462.1"
                     /translation="MRELHALRPDRVRVIPGADGWPEAFEYAVAGQTVRIEGEATPGI
                     RAILHQKLFHPANDHYGLSPIEAAATAIDIHNAASRWNKALLDNSARPSGALVYTARD
                     GQLSPEQFERLRSELEAGFQGAANAGRPLLLEGGLDWKAMSLSPKDLDFIEAKNAAAR
                     EIALAMGVPPAPRSPRPAKA"
     gene            complement(3367..3762)
                     /locus_tag="PK264_21410"
     CDS             complement(3367..3762)
                     /locus_tag="PK264_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78463.1"
                     /translation="MKSERRERYYNRTVTECIEQFVGELPSDAVGLWQILPMAREGFG
                     FEGVEQIDFIRTAIKALLEAGAIPVRYGGRTHDWVQQHQYGKSIDEIAQSVIEEWASY
                     GDMKFDEIKVYGEAVWFARPRADPRYIRE"
     gene            complement(3759..4511)
                     /locus_tag="PK264_21415"
                     /pseudo
     CDS             complement(3759..4511)
                     /locus_tag="PK264_21415"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4649..5044)
                     /locus_tag="PK264_21420"
     CDS             complement(4649..5044)
                     /locus_tag="PK264_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78464.1"
                     /translation="MSTLDRDTYDGLTQDENIAEFIKELENDAVSMSQIVFLSRDGFH
                     MQPDEATDFIHKSVFALVEAGAVPVRFGGPSHDWVRQHQYGTSPENIANAVVREWQGY
                     GDLRFDEVKVYGEAVWFARPRTNPTYIRE"
     gene            complement(5350..>5766)
                     /locus_tag="PK264_21425"
     CDS             complement(5350..>5766)
                     /locus_tag="PK264_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78465.1"
                     /translation="AFWRQTVIPLATRTGRALATWLAPGEGLALRPDLDAIEALSSER
                     EALWSRLEAASFLTANEKRAAAGYGEIDDDALAAKFNPYHDALGRFTTGPGLGDVGQR
                     RGPSDGKPKQPGPPEPAEPKPGQPKAPQATDKSKSP"
BASE COUNT          947 a         1783 c         1939 g         1097 t
ORIGIN      
        1 agggcaggtc ctggctcggc tcgggcccgg tgagcaggcc gccagggtcg ccaaggcgcg
       61 cgtcacgatc gaggtcgccg aggtgaacat caagcgggcg gaagccaatc tcgccaaggc
      121 gcgcgccgtg ctcgaccaga ggcaggagac caaccggcgc aagcagacgc tcgctggccg
      181 ggatatcgtc tcgcggcagg cggcggaaga ggccgcgcgg gacgaggcca tcgccatcgc
      241 cgacgtcgca atcgctgaaa gcgagatcag cagtaccaag gcccagctcg cggacgcgcg
      301 cgcgaccctc gcgttcgagg aggccatgct cggccatcgc acgctcgtcg cgccgtatga
      361 tgcgatcgtc atcgagcgtc acaaggagct tggcaccgtg gtgaaggcgg gagacccggt
      421 cttcacgctc gttgcgctcg acggatattg ggggctcgcc ttcgtcgacg aggcaagggc
      481 gggcgccatc catgaaggcc agcccgtggt cgcacgactg cgatccctgc cgcgagatgc
      541 ctttcacggc aaggtgctgc gcatcggcct cgaaagcgac cgtgtgaccg aggagcgccg
      601 tgtctacgtg cggggcgaca atcctccacc gcgcgtcttc ctcggcgagc aggtcgaatt
      661 cctcatcacc gtcacgtccc tcgaacgggc acttctcgtt ccggaggcgg cggtacacgg
      721 ctacaacggc cgcgaaggcc gtgtctggac gctcgagagc ggtcggctcg tgcgccgcct
      781 cgtccagttc aggcatcgca ccgaggacgg ccggctcgag atcgtcgagg gtgtcccgga
      841 cggggttctc gtcgtcaccc ggtccgatcc gggctggcga gagggccagg ccgcacgggc
      901 gagcgaggta aagcccaaat gaacctcgct tatcgcgacg tccgccacaa cctcggccgc
      961 ttcctgctga cctgcgtcgg gctcgggctc ctgctcggcg tggtgatggc catgatcggt
     1021 atctaccggg ggctgatcgt cgatgcgctg accatcgcgc gcgccccggc agtcgatctc
     1081 tgggtcgtcg aatccggctc tcgaggcccc ttcgccgagg catctcgcat accgggagac
     1141 gtgcgcgagg cggttgcccg gatcgccggc gtcgccgagg cgggcagtat cacctaccag
     1201 acgatcgagg cggagcatcg cggcagcaag ctcaggcttt atgttatcgg ctatgagccg
     1261 agccgacccg gagctccgcc agagatcgtc gatgggcgcg gcatcgcgcg aagccacttc
     1321 gagatcgtgg cggatcgaac gtcggggctg acacttggcg atcgcatcac gctcggccgc
     1381 aatgtcttta ctgtcgtggg cctgacccgg cagcaagtga gctccggcgg cgatccagcg
     1441 gcctacatca cgcttgcgga tgcgcagcgc ctgcaattcg atctcgcccc gccggccgtt
     1501 cgcgtcgaga tcgcgcgcgg tgcgaccgcg ggcagccgtg acatcgtcaa cgccgtcatc
     1561 gcccggctgc agccgaacgc gtcaccggat gcggtggcgg aaacgatccg gcgctggcgg
     1621 cacctctcgg cgatcaccca agagggccag gagcagatcc tcacacagtc gctcgtcgac
     1681 agggctcgcc gccagatcgg cttgttcacg tccctcctgc tcgtcgtctc ggcggtgatc
     1741 atcgctctca tcatctatac gatgacgatg gagaagctga agcagatcgc cacgctcaag
     1801 ctgatcggcg cgcccgaccg cacgatcatc gccatgatcg tgcagcagtc gttggcgctc
     1861 ggctcgatcg gattcgcggc gggcgctgcc ttgatcttcg cgctcaaaga caggtttccg
     1921 cgacgcgtcg tgctcgagcc cgacaatgtc gtgacgctgg gcttcatcgt tctcgccgta
     1981 tgcatcgtgg cgagcgggct cggcgtgcgt gcggcaagca aggtcgatcc ggcgacggcg
     2041 ctcgggggct gacatgggat cgaagcaggg tgcgctcgtc gatatattgc gggtctcgaa
     2101 gcatttcggc gacggcgacg cgcgggtcga cgcacttcgt gaggtgtcta tctccgtgca
     2161 ccctggcgag gtggtcgccc tcctcggccc gtccggttcg ggaaagacca cgctcctcaa
     2221 cgtgatcggc tgcatcctcg atccgtcggc cggctcgatg cagctcgacg gcgagcgtgt
     2281 cctcgagaac ggtcgctggc tccggtccga tctccgccgt ctgcgcctcg acaagatcgg
     2341 cttcatcttc cagttccata atctgatccc gttcctcgat gcgacggaga acgtcgccct
     2401 cgtctgcgaa ctcgcgggcg acgatgcgat ctcggcacgt gcgaaagcgg tcgaactgct
     2461 cgactatctc gaagtctcac accggcgaag cgccatgccg gcgaagctct cgggtggcga
     2521 agcgcagcgt gtcgcgatcg cgcgggcgct cgccaacagt ccgcgcatca ttctcgccga
     2581 cgagccgacg gcggcactcg attcgaagcg tgcggggatc gtgatggacc tcttgcgcaa
     2641 gctcgcccgc gagcgcagcg cggccatcat cgccgtcacg cacgacgaga agattttcga
     2701 ccgcttcgac cgtctgcatg tcttgcgcga cgggatgctc gtcgagacgc gccagggcgc
     2761 ggggccttga ggcgctgtca cgccttcgcc ggccgtggtg accggggggc aggcggcacg
     2821 cccatggcga gggcgatctc gcgggcggct gcgttcttgg cctcgatgaa gtcgaggtcc
     2881 tttggcgaaa ggctcatggc cttccagtcg aggccgcctt cgaggaggag ggggcggcct
     2941 gcgttggcag cgccctggaa gcccgcttcg agctcggagc gcaggcgctc gaactgttca
     3001 ggcgagagct ggccgtcgcg ggcggtgtag acgagggcgc ccgaggggcg ggcggagttg
     3061 tcgaggagag ctttattcca gcggctcgcg gcgttgtgga tgtcgatcgc ggtcgcggcg
     3121 gcctcgatgg gggagaggcc atagtgatcg ttggcggggt ggaagagctt ctggtggagg
     3181 atcgcgcgga tgcccggcgt ggcctcgccc tcgatgcgga cggtctggcc tgcgacggca
     3241 tactcgaagg cttcgggcca gccgtcggcg ccggggatca ccctcacgcg gtcgggccgg
     3301 agcgcgtgaa gctcacgcac ctccgacccg accgctacgg cctcgagaat ctaactcaga
     3361 cgggtgctac tcgcgaatgt agcgcggatc tgctcgtggt cgagcaaacc agaccgcctc
     3421 gccatacacc ttaatctcgt cgaacttcat atctccgtag cttgcccatt cctcgatgac
     3481 cgactgggcg atttcatcta tcgacttgcc gtattggtgc tgttggaccc agtcatgagt
     3541 acgcccacca tatctcacag gtatcgcgcc cgcctcgagg agagccttga tcgccgtccg
     3601 aatgaaatcg atttgctcca cgccttcgaa cccgaagcct tctctcgcca ttggcaatat
     3661 ttgccataga cctactgcgt ccgatggcaa ttcacctacg aattgctcga tgcattcggt
     3721 tactgttcga ttgtagtacc tctctcgccg ctcacttttc atcgctgatg caccttgtaa
     3781 ccgcgtggta gtcgtggatg gttggattta acaatttcta acgtgtgtcc gctactagac
     3841 cttctttctc cgacaatagt accgtcgagt ctctgatacc acacaccatc gtaatccgcg
     3901 ggggcggctg tgggcactgc accagctagc agtctcgccc gcaattgcgc atagactgta
     3961 gtcgtcactg tccgaatatt gtctcctgag ccaggctccc ttctgccaac ctcctgtccg
     4021 ccaggcaaca gaatgtcatg aaggggctcg ggggcaccag gaggtgcttt tcctgaaggc
     4081 tttcgaggat cgggctgctt gttggtgtct ggcggcggcg ccggcttggc tggggctggc
     4141 gtaggtccgg gcttgggctt ctgcggcggc ccagcggcag ggatcgttcc ctcaccctct
     4201 cccgtccagc ggccgtggtc gtcgcgcggt tggctggtgt tgaagccggc ttttgcggcg
     4261 agggcgtcgt cgtcgatctc gccgtagccg gcggcggcgc gcttttcgtt ggcggtgagg
     4321 aaggacgcgg cttcgaggcg ggaccagagc gcttcgcgct cggaagagag ggcctcgatg
     4381 gcgtcgaggt cggggcgcag cgcgaggcct tcgccggggg cgagccaggt ggcgagggcg
     4441 cggcccgtgc gcgtcgcgag ggggatgacg gtctggcgcc agaaggcgcg cgtcgcctcc
     4501 tggaagttcg agtaggtgtt gtcgcatcag tcgagatgcg cgcctacgtg cgcggggtat
     4561 gctgcagagc agtcgagatt gcacggcgcc aggggctagg agggaacgaa ctggagtccc
     4621 agcggaacat tgcggtctat aggaactctc attcgcgaat gtaggttgga ttggttctag
     4681 gccgagcaaa ccaaacggct tccccgtata ccttcacttc atcgaatctg aggtcgccgt
     4741 aaccctgcca ttctctgacc acggcattag caatattttc cggtgacgtg ccgtattggt
     4801 gttggcgaac ccagtcatgt gacggaccac caaatcgcac cggaactgcg ccagcttcga
     4861 caagggcgaa taccgatttg tgaatgaagt cagtggcctc atcgggttgc atgtgaaatc
     4921 cgtctcgact caggaagacg atctgactca ttgatacggc atcattctcc agctccttga
     4981 tgaactctgc aatgttttcg tcttgtgtga ggccatcgta agtgtcgcga tcaagcgtgc
     5041 tcatctggtg tggaccttga aatttgctcg tattgccgga tgactgctgc gaattacgtc
     5101 gagtgtgggt ccgctttccg ggctaatcct ctccccgact attgaaccat cgcgcctcaa
     5161 ataccaggtg ccattgtagc ttgacggcga aggataggga aaggcgccat tgaggatccg
     5221 acgccggatc acggagtatt cagcagctga tacggtgcga accctcgtat tgctgccggc
     5281 gcgtactcca acgaatactc cgcctggcat caatatatcg cgaagtggct ccggcgcgga
     5341 aggtcgatct tagggtgact ttgacttgtc cgtagcttga ggagccttag gctgaccagg
     5401 cttcggctcg gcgggctcgg gcggccctgg ctgctttggc ttgccgtccg acggtccgcg
     5461 gcgctggccc acatcaccca ggccaggccc tgtcgtgaaa cggccgaggg cgtcgtggta
     5521 ggggttgaat tttgcggcga gggcgtcgtc gtcgatctcg ccgtaaccgg cggcggcgcg
     5581 cttttcgttg gcggtgagga aggacgcggc ttcgaggcgg gaccagagcg cttcgcgctc
     5641 ggaagagagg gcctcgatgg cgtcgaggtc ggggcgcagc gcgaggcctt cgccgggggc
     5701 gagccaggtg gcgagggcgc ggcccgtgcg cgtcgcgagg gggatgacgg tctggcgcca
     5761 gaaggc
//
LOCUS       DAORNH010000295         5756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10319_length_5756_cov_2.699176, whole genome shotgun sequence.
ACCESSION   DAORNH010000295 DAORNH010000000
VERSION     DAORNH010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10319_length_5756_cov_2.699176"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PK264_21430"
     CDS             <1..262
                     /locus_tag="PK264_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78466.1"
                     /translation="SDFDRRFIAVCTSQGLARARCDCLLGVFKEHIADDNLELIIGYF
                     EDPAGFQDRIAELDLDPERAEALREEIDEAVAASREECGTGP"
     gene            complement(267..770)
                     /locus_tag="PK264_21435"
     CDS             complement(267..770)
                     /locus_tag="PK264_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78467.1"
                     /translation="MLADIVDRHLIIIAGALIVIVWAIAGIFHRMIKARTKRRMLDTV
                     RDLAGRGMSADDISRIVGSRHEAPGETERHEALTELAEEMLESGWSPEAAAAAVRPLT
                     DLAGEASRDPAKAARFAAVMPMIDRMLEERRDARDVQKMIEAFIAGSPGAATGTPPRS
                     FLPPRNA"
     gene            948..1943
                     /locus_tag="PK264_21440"
     CDS             948..1943
                     /locus_tag="PK264_21440"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HRD78468.1"
                     /translation="MSEPRPMTAAELRAKMRLPLFGSPLFIISNPKLVIAQCKAGVVG
                     SFPALNARPQSLLDEWLHRIKEELAAHDRRHPDRPSAPFAVNLIVHKSNDRLEADAAT
                     CLKHKVPLLITSLGAREDIYTTFAKSGTLVFHDIINITFARKALEKGAGGLVAVCAGA
                     GGHAGRLSPFGLIQEIREFYDGPLALSGAIAHGGSILAAEALGADFAYAGSMFIAASE
                     ANAVEGYKADIVAGTADGIVYTNLFTGVHGNYLRSSIARAGLDPDNLPVSDPSKMNFG
                     TNRPARAWKDIWGSGQGIGAVKQIEPVAAMVERMEREYKAAKAAIARGARAPAGA"
     gene            complement(1949..2167)
                     /locus_tag="PK264_21445"
     CDS             complement(1949..2167)
                     /locus_tag="PK264_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78469.1"
                     /translation="MRRIIQAAAIAALLAAAQPLASDPAEAGRRTFVQRKIVDGRFCT
                     IAYEQWCSRLCGRPKEIYRHCLPIKVGK"
     gene            2471..3106
                     /locus_tag="PK264_21450"
     CDS             2471..3106
                     /locus_tag="PK264_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HRD78470.1"
                     /translation="MMTMSADRVGKASGARSPAMLRRAVAALTVVALAGLAPTSPIGA
                     QAPKPAPAAAAPAKGKGAEPPVTPPDGVVKSQFGDWQLVCKGPPPGAKQEVCALVQSV
                     TAEDRSNVGLTVYIQKFSSGQTVLRVFAPLGVLLPRGLGLKIDNADVGNAPFLRCHSF
                     ACYAQVVLEEKLLAQLKTGKTAVFIIFQTEEQGIGIPISLAGFPQAIGALK"
     gene            3283..4809
                     /locus_tag="PK264_21455"
     CDS             3283..4809
                     /locus_tag="PK264_21455"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRD78471.1"
                     /translation="MLALMSRATSFFRDRRGNVAIIFAMTTIPVAGVTGAAIDYGRAY
                     VAHMHLQSATDAAAMAAASAANKTTGERESIAVAMFKANVAGNSAIANAVPQVTQSDG
                     VTKVEVSAAVGTTFMRLLNVNDMLIEASTTVQTAGKKLELALMFDVTGSMNDTTSTGN
                     SKIVDAKAAALDLLDIMLPETGLATRRIALIPFASKVKVPADVAAAATGSPATKTESS
                     TKSTTTTTWSLSSTDFDWLKLSSCEDRLKDYYDDHGGMSSSAAQTKAEDECASFTKSK
                     IDNKWRYKTPEVVSTSTTTTTTTYTDKYINPCMIERLGSAPSRYEDDAPGTGFTAVYS
                     TTKTADVSCPPSTTLIPLTSDRTLLSNTISALTTGSSTAGHLGTAWSYYSISPNWSSA
                     FPVESAPVAYDDTGTIKAAILMTDGEYNTTHCGTSCASASEQAIAICTEMKGRGIQVW
                     TIGFGMSTNPSDPARQTLVECADPGRYFFPYNGDELRAVFATIGQSLTDTVTKPRIVR
                     "
     gene            complement(4970..>5756)
                     /locus_tag="PK264_21460"
     CDS             complement(4970..>5756)
                     /locus_tag="PK264_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HRD78472.1"
                     /translation="VQWASELEREECSIDELWVSTKCGESDTTTGLGSCPTVGNMYDK
                     LIPKGVYGVFGETSEITGAEHIARERAISKAIGDKWYKVWKAYQDDVIEANKVDDLSE
                     SQPTKGNIAGGLSSIEEKALGNLEKIGRECKYIDVLGPAEEPAKGPGLYYMDTSSAAA
                     ECVTLMAAGGYVVHTFPTGQGNVIGNPIVPVIKISANPKTLRTMPEHIDVDVTGILRR
                     EMTLDAAGDTLIDMIIRTANGRLTAAEALGHREFSLTKLYRSA"
BASE COUNT         1022 a         1953 c         1831 g          950 t
ORIGIN      
        1 gtcagacttc gatcgccggt tcattgcagt ctgcacgtcg cagggcctcg cacgagcgcg
       61 atgcgattgc ctgctcggcg tgttcaagga gcacatcgca gacgataacc tcgagctgat
      121 catcggctac ttcgaggacc cggcgggctt ccaggatcga attgccgagc tcgatctcga
      181 cccggaacgc gcggaggcgc tgcgcgagga gatcgacgag gccgtggccg cgagccgcga
      241 ggaatgcggc accggaccgt agccgatcag gcgttcctcg gtggcaggaa cgagcgtggc
      301 ggcgtgcccg tcgcggcgcc cggcgagcct gcgatgaacg cctcgatcat cttctgcacg
      361 tcgcgggcat cgcggcgctc ttcgagcatc ctgtcgatca tcggcatgac cgcggcgaag
      421 cgggccgcct tggctggatc gcgcgaagcc tcgcccgcga gatcggtgag cgggcgcacg
      481 gcggcggccg ctgcctcggg cgaccagccg gattcgagca tctcctcggc aagctccgtc
      541 agcgcctcgt gacgctccgt ctctcccggc gcctcatgcc gcgagccgac gatgcgggat
      601 atgtcgtccg cgctcatgcc gcggccggcg agatcgcgga cggtgtcgag catgcgccgc
      661 ttggtgcggg ccttgatcat ccggtgaaag atgccggcaa tggcccatac gatcacgatc
      721 agggcgcccg caatgatgat caggtgccgg tcgacgatgt cggcaagcat gagtgtattt
      781 ctcctggagg tcggacgaga ccgatcctgc aactattgga ggacccagac attgatgtgc
      841 gccctatgcc gcgcgcaaag agtgttgcaa gtccaggcgc ggagcgccag ggtctcatgg
      901 ggcctcgagt gtcggcccga tcacaccaag gcgagggaga agcgccaatg tccgagccac
      961 gtccgatgac cgccgctgag ctgcgggcaa agatgcgcct ccccctgttc ggctcgccgc
     1021 tgttcatcat cagcaacccc aagctcgtga tcgcgcagtg caaggccggt gtcgtcggca
     1081 gctttccggc cctgaacgcc cgcccgcagt cgctgctcga cgagtggctg caccggatca
     1141 aggaagagct tgccgcgcac gatcgccgtc accctgatcg gccctcggcg ccgttcgcgg
     1201 tgaacctcat cgtgcacaag tcgaacgatc ggctggaggc ggacgccgca acgtgcctaa
     1261 agcacaaggt gccactgctc atcacctcgc tcggcgcgcg cgaggacatc tacacgacct
     1321 tcgccaagtc cggcacgctc gtcttccacg acatcatcaa cataacgttt gcgcgtaagg
     1381 ccctcgagaa gggtgccggc ggcctcgtcg ccgtctgcgc cggtgcgggc ggccacgctg
     1441 gacgcctctc gcccttcggg ctcatccagg agatccgcga gttctacgat ggcccgctcg
     1501 cactctcggg cgccatcgcc cacggcggct cgatcctggc cgcggaggcg cttggcgcgg
     1561 atttcgctta cgccggctcc atgttcatcg cggcgagcga ggccaatgcg gtcgaaggct
     1621 acaaggcgga catcgttgcc ggtactgcgg acggcatcgt ctacacgaac ctcttcacag
     1681 gcgtgcacgg caactacctg cgttcctcga tcgcacgggc cgggctcgat cccgacaacc
     1741 tgccggtcag cgatccctcg aagatgaact tcggcaccaa ccggccggcc cgtgcctgga
     1801 aggacatctg gggctccgga cagggcatcg gcgcggtgaa gcagatcgag cccgtggccg
     1861 cgatggtcga gcgcatggag cgcgagtaca aggccgcgaa ggccgcgatc gcacggggcg
     1921 cacgggcgcc cgccggcgcc tgagcattct atttgccgac cttgatcggc aggcagtgcc
     1981 gatagatctc ctttggccgg ccgcacaagc gcgagcacca ttgctcgtag gcgatggtac
     2041 agaagcggcc gtcgacgatc tttctctgaa cgaacgtgcg ccgaccggcc tcggcgggat
     2101 cagaggcgag cggctgagcc gcggcgagca gcgcagctat cgcggcggcc tggatgatgc
     2161 ggcgcaaggt aatctccatc tctctcttga acaagtcgac aggcaccggc cgctctgccg
     2221 aggcgtcgcc gggcctgtcg gggctgctgc ctcacttgcg tgatcattga tcacatcaga
     2281 gccggaactg ctgcgaggca agggcaatcg tcggcgcgcc gccgccagtg tgacgcgcca
     2341 ggcggtccac agcctcgccg gcgcaatccc acttaagccc gaattctcgg gcagtccgat
     2401 cggctatcgg gtatgcgacc gggcgatagc atgtgaccgc gctagcggcg cggtggagac
     2461 gtggtgcacc atgatgacga tgagcgctga tcgagtaggc aaggcatccg gcgcgcggtc
     2521 gcccgcgatg ctccggcgcg cggtcgcagc tctcactgtt gtcgcactag caggcctcgc
     2581 gccgacgtca ccgatcggcg cgcaggcgcc gaaaccagcg cccgcagcag cagcgcccgc
     2641 caagggcaag ggcgccgagc cccccgtcac cccgcccgat ggcgtggtca agtcgcaatt
     2701 cggcgactgg cagctcgtct gcaagggacc gccgcccggc gcgaagcagg aggtctgcgc
     2761 cctcgtgcag agtgtcaccg ccgaagaccg atcgaacgtc ggtctcaccg tctacatcca
     2821 gaaattctcg agcggacaaa cggttctgcg cgtattcgcc ccacttggcg tgctgctgcc
     2881 tcgcggcctc ggactcaaga tcgacaatgc cgacgtcggc aacgcgccgt tcctgcgctg
     2941 ccacagtttc gcctgctatg cccaggtcgt gctcgaggag aaactgctcg cccagctgaa
     3001 gaccggcaag acggcggtct tcattatctt ccagacggag gagcagggca tcggcatccc
     3061 gatctcgctc gcgggcttcc cgcaagctat cggcgccctc aagtagcggc gcagcagaaa
     3121 taagcgcagg ggccggcgat cggtgcaatt tggctcaatt cccgcacgat ttgttcacat
     3181 ttcctgaacc ccgccttcat ccgaccgatg acatggttcc ccgatcggaa accagcgccg
     3241 cgccggccaa aggcccgacg gcgccgaggg cggggaattg ccatgctggc actgatgagc
     3301 cgggcgacgt cgttttttcg cgaccgccgg ggcaatgtcg cgatcatttt cgcgatgacg
     3361 acgatcccgg tcgcaggcgt aacaggtgcc gccatcgact acggccgcgc ctatgtcgcg
     3421 cacatgcacc tccagtcggc gacggacgct gccgccatgg cagctgcgtc ggctgccaac
     3481 aagacgaccg gcgagcgtga atcgatcgcc gtcgccatgt tcaaggccaa tgtcgccggc
     3541 aacagcgcga tcgccaatgc agtgccgcag gtgacgcagt ccgacggcgt caccaaagtc
     3601 gaggtgagcg ctgctgtcgg caccacgttc atgcgcctcc tcaacgtcaa tgacatgctc
     3661 atcgaggcat cgaccacggt gcagacggcg ggcaagaagc tcgagctggc gctgatgttc
     3721 gacgtcacgg gctcgatgaa cgacacgacg tcgaccggca attcgaagat cgtcgatgcc
     3781 aaggcggcgg ccctcgacct cctcgatatc atgctgccgg agacgggcct cgcgacgcgc
     3841 cgcatcgcgc tcatcccgtt cgcctccaag gtaaaggtgc cggcggacgt cgccgcggcc
     3901 gcaacgggct cgcccgcaac caagaccgag agcagcacca agtcgacgac cacgacaacc
     3961 tggtccctgt cgtccactga tttcgactgg ctgaagctga gctcctgcga ggatcgcctc
     4021 aaggactact acgacgatca cggcggcatg tcgtcgagcg cggcccagac caaggccgag
     4081 gacgagtgcg cctcgttcac caagtcgaag atcgacaaca agtggcgcta caagacgcca
     4141 gaggtcgtct ccacctcgac gacaacgacg acgaccacct acacggacaa gtacatcaac
     4201 ccctgcatga tcgagcggct cggctcggcg ccgtcacgct acgaggacga cgcgcccggt
     4261 accggcttca cggcggtcta ttcgacgacc aagacggcgg acgtgagctg cccgccgtcc
     4321 acgaccctga tcccgctgac ctccgatcgg acgttgctca gcaacaccat cagcgcgctc
     4381 accacgggca gctcgactgc gggccacctc ggcacggcgt ggtcgtacta ctccatctcg
     4441 ccgaactggt cctcggcctt tccggtcgag agcgctcccg ttgcctacga cgacacgggc
     4501 acgatcaagg ccgcgatcct catgacggac ggcgagtaca ataccaccca ttgcggcacg
     4561 tcatgcgcga gcgcgtccga gcaggccatt gcaatttgca ctgagatgaa gggccgcggc
     4621 atccaggtct ggacgatcgg cttcggcatg agcacgaacc cgagcgaccc agcgcgccag
     4681 acgctcgtcg aatgcgccga tccgggtcgc tacttcttcc cctacaacgg cgatgaactt
     4741 cgcgccgtat tcgcgaccat cggccagtcg ctcaccgaca cagtgacgaa gccgcgcatc
     4801 gtcaggtagg cggggcccga atgaattggt ggccgaatca atcagggacc ggcacttgtc
     4861 ggctcaccgc gtcccccgac gggtatcgag ccaaagcgcc ggtcccctcc ccctgagcag
     4921 gcctgaaaga gcgcggtgac ggcacgagcc ggcgccgcgc atccgaacgt caggcgctcc
     4981 ggtagagctt cgtcagcgag aactcgcggt ggccgagagc ttccgccgcc gtgagccgtc
     5041 cgttcgctgt acggatgatc atatcgatca gcgtatcgcc cgccgcgtca agggtcatct
     5101 cgcgacgcag gatgcccgtc acgtcgacgt cgatgtgctc aggcatggtg cgcaaggtct
     5161 tcgggttcgc cgagatcttg atgactggca cgatcggatt accgatgacg ttgccctgcc
     5221 cagtcgggaa ggtgtgcacg acgtagccgc cggcggccat cagcgtcacg cattcggccg
     5281 cggccgacga ggtgtccata tagtagaggc ccggcccctt cgccggctcc tcggctggac
     5341 cgagcacgtc gatatacttg cactcgcggc cgatcttctc gaggttgccg agtgccttct
     5401 cctcgatcga ggagagcccg ccggcgatgt ttcctttggt cggctggctc tcggagaggt
     5461 catcgacctt gttggcctcg atgacgtcgt cctggtaggc cttccagacc ttgtaccact
     5521 tgtcgccgat ggccttgctg atggcgcgct cacgcgcgat gtgctcggcg cccgtgatct
     5581 cgctcgtctc gccgaagacg ccgtagacgc ccttggggat cagcttgtcg tacatgttgc
     5641 cgaccgtcgg gcaggagccg aggcccgtcg tggtgtcgct ctcgccgcac ttggtcgaga
     5701 cccacagctc gtcgatcgaa cactcctcgc gctcgagctc ggaggcccac tgcacg
//
LOCUS       DAORNH010000296         5747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10340_length_5747_cov_2.611560, whole genome shotgun sequence.
ACCESSION   DAORNH010000296 DAORNH010000000
VERSION     DAORNH010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10340_length_5747_cov_2.611560"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK264_21465"
     CDS             complement(<1..194)
                     /locus_tag="PK264_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78473.1"
                     /translation="MVGASARLLGPGDRVRPDRDRRRWRWRLNLLPPLNEQRERPRCR
                     VAPGYRFAIPDDCAWVNYIR"
     assembly_gap    287..390
                     /estimated_length=104
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <391..1176
                     /locus_tag="PK264_21470"
     CDS             <391..1176
                     /locus_tag="PK264_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78474.1"
                     /translation="CRRWESKVIAPPVRPTLPATAPTPEELKLLRAMDETVRGRGSLI
                     EFGGNGRYTVMADRFAADVRIYTGQSYHPALCSGAGDMLKFYAETLAPLKSRIKVVAD
                     MDRRAQTRARAAVGSALADLARPSTGDLAASETPKSDAPSATVATASDVPPPQPSVPE
                     TIEAMTAALADIALPEGAAQAIKANESVLQRLKAFAEWLMSEPGLALTGVRRETVLVA
                     LRALEIAAHVEAMQARYVVLETALLGNLLVLKESQRRTCTCGT"
     assembly_gap    1389..1442
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1535..3535
                     /gene="parE"
                     /locus_tag="PK264_21475"
     CDS             1535..3535
                     /gene="parE"
                     /locus_tag="PK264_21475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRD78475.1"
                     /translation="MAGSRDLFARLEGEFEGKRGRQGKSAEESYTAADIEVLEGLEPV
                     RRRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAGHATWIEVRLEPDGFLSVADNGRGI
                     PVDPHPKFPKKSALEVIMCTLHSGGKFDGKAYNTSGGLHGVGVSVVNALSETCEVEVA
                     RGQQLYRMVFSRGAPQTKLEKLGSIMNRRGTKVRFRPDEQIFGRGAAFKPARLFKMAR
                     SKAYLFGGVEIRWFADAALCSDGTPTEAVFHFPGGLKDCLAGDIEGRTRVTKEVFAGR
                     IDKEGGHGSLEWACCWISDEDGFLNSYCNTIPTAEGGTHEQGFRTALAKGLRAYGELT
                     NSKKASQITADDVFGTAAGMLSVFIREPEFQGQTKDKLATVEAGRIVEAAVRDAFDHW
                     LADSPQQATRLLEWTIEQSEERLKRRREKEVSRQSATRKLRLPGKLWDCSSTSGDTEL
                     FIVEGDSAGGSAKQARDPKTQAVLPLRGKILNVASATSQKLAANQQLTDLLQALGVGT
                     GSKYRAEDLRYDRIIVMTDADVDGAHIASLLITFFYQEMPEMIEGGHLYLAVPPLFKL
                     THGAKTIYARDDAERLRLLKSEFNANAKVEVTRFKGLGEMNASHLKETTMDPRRRTLL
                     KVEIVDDRAHLSQAVSDLMGTKPEARYKFIQERAAFAKDLDI"
     gene            3595..3933
                     /locus_tag="PK264_21480"
     CDS             3595..3933
                     /locus_tag="PK264_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRD78476.1"
                     /translation="MSFRRRASAAALLVALPAIGTVVAQDGAQVARGRQLLEAQCARC
                     HAVGASDASPVKQAPALRQLRRRYPVDALAEALAEGIITGHPGMPEFAFQANDVAAII
                     AYLKSLPDPR"
     gene            4091..5203
                     /locus_tag="PK264_21485"
     CDS             4091..5203
                     /locus_tag="PK264_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HRD78477.1"
                     /translation="MKAAVLHEVNKPLVIEDVDVKKPGPREVLIRTAFAGLCHSDLHF
                     MEGLYPHPLPVVLGHESAGVVEAVGSEVHYVQKGDHVITCLSVFCGTCENCTSGRPVI
                     CTDTSVKMLPGQAQRLSLKGNHVNTFTNLSSYAEMMLVHENAIVKIDKDFPLDRAALI
                     GCGVITGFGAAVNTAKVAPGETVAVIGCGGVGMAAVNGAAVAGAGRIFAVDTNPAKLQ
                     MAAKIGATDLINPRDGDPVERIRAALGGKPSDGVHHAIECLGLKVTAEQCFQMLRHGG
                     TATIVGMIPFGTKIELHGFDFLREKKVQGSSMGSNRFRTDMPRLMEFYRRGKLHLDDF
                     VSAHIKLGEINEGFASMKAGKVIRSIIDFGVVKARP"
     gene            5334..>5747
                     /locus_tag="PK264_21490"
     CDS             5334..>5747
                     /locus_tag="PK264_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRD78478.1"
                     /translation="MRYEAPDSLEAAAKLLSSESGEARVLAGGTDLLVQMRADVVHPV
                     LVVDIKRIAETRRIVEEGGNFRIGAAVTGAELKEHAALKAALPGLVEAANLIGSTQVQ
                     GRATMGGNLCNGSPAADSVPALIAAGAKASIVGPGG"
BASE COUNT          988 a         1841 c         1907 g          853 t
ORIGIN      
        1 cagcggatgt agttgaccca cgcgcagtcg tcggggatgg cgaagcggta gcccggcgcg
       61 acccggcagc ggggccgttc tcgctgctcg ttcagcggcg gcagaaggtt cagccgccac
      121 cgccaccgcc gccgatcccg gtcgggccgg acgcggtcgc caggaccgag gaggcgtgcg
      181 ctcgcgccta ccattccgaa ctctcgaagg tcgcggccgc cgtgcccgcg atgggaaagg
      241 ccgtacgctc catgaaggcc gacgtggcag gcgcgacggg cggctgnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgccggcgct gggaatcgaa ggtgatcgcg
      421 ccgccggtgc gcccgacgct gccggcaacc gcgccaacgc ccgaggaatt gaaattgctc
      481 cgcgccatgg acgaaacggt gcgcgggcgt ggcagcctga tcgagttcgg cggcaacggg
      541 cgctacaccg tgatggcgga ccgcttcgca gccgacgtcc gcatctatac cgggcaatcc
      601 taccatccgg cgctctgcag cggagcgggc gacatgctga agttctatgc cgagacgctg
      661 gcgccgctga aatcgcgcat caaggtggtc gccgacatgg atcgccgcgc ccagaccagg
      721 gctcgcgccg cggtcggctc cgcgctggca gatcttgcgc ggccgtcgac cggagatctt
      781 gcggcctccg aaactcccaa gtctgacgct ccgtccgcaa ccgtggcaac cgcgtccgac
      841 gtgcctccgc ctcaaccgag cgtgccagag acgatcgagg cgatgacggc cgcgctcgct
      901 gacattgcgt tgcccgaggg ggccgcacaa gccatcaagg caaacgagtc ggtacttcag
      961 cgcctcaagg catttgcgga gtggctgatg agcgagccgg gcctcgcact caccggcgtg
     1021 cgtcgcgaga cggtgctggt ggcactcagg gcactcgaga tcgcggccca cgtggaagcg
     1081 atgcaggccc gctacgtcgt actcgagacg gcgctgctcg gcaatctcct cgtcctcaag
     1141 gaaagccagc gccggacctg cacctgcggc acctgatggc cgcatccgat gcccgaagtt
     1201 cgcgccgacg aatggtgcgg cgctgcaact tgggtgtgga aaaggcgtag gctcgggcgc
     1261 aggacatgcg ctcgcgccac agcggcgccg cgccgacttg caaaatggtg gagcgggtgc
     1321 gacctgaggc gacagtggcg aggctcctgt cgagtcgccc gacgtgtggc aaccgggcgc
     1381 cggttcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnatcggtca tggcgtgccg agtgacgggc agccgagtgt cgccagccga tgcaaggccg
     1501 ggccaggaat gcgagccgag ggaagggacg acagatggcg ggttcacggg accttttcgc
     1561 gcggctcgag ggcgagttcg agggcaagcg cggccggcag gggaagtcgg ccgaggagag
     1621 ctataccgcc gccgacattg aggtgctcga agggctcgag cccgtgcgcc ggcgtcccgg
     1681 catgtacatc ggcggcaccg acgagaaggc gctgcaccac ctctttgccg aggtgatcga
     1741 caactccatg gacgaggctg tcgcgggcca tgcaacctgg atcgaggtgc gactcgagcc
     1801 ggatggcttc ctctcggttg ctgacaacgg ccgcggcatc ccggtcgatc cgcacccgaa
     1861 gtttccgaag aagtccgcgc tcgaagtgat catgtgcacg ctgcactcgg gcggcaagtt
     1921 cgacggtaag gcctacaaca cgtcaggcgg cctgcacggc gtcggcgtct cggtcgtgaa
     1981 cgccctgtcg gagacctgcg aggtcgaggt cgcacgcggc cagcagctct atcgcatggt
     2041 gttcagccgc ggtgcaccgc agaccaagct cgagaagctc ggctcgatca tgaaccggcg
     2101 cggcacgaag gttcgattcc ggccggatga gcagatcttc gggcgcggcg ccgccttcaa
     2161 gccggcccgc ctcttcaaga tggcgcgctc gaaggcctat ctcttcggcg gcgtcgagat
     2221 ccgctggttc gcggatgcgg cgctttgctc ggacggcacg ccgacggagg cggtgttcca
     2281 ctttccaggc ggtctcaagg actgcctcgc cggcgacatc gaggggcgca cacgcgtcac
     2341 caaggaggta ttcgccgggc ggatcgacaa ggagggcggt cacggctcgc tcgaatgggc
     2401 gtgctgctgg atcagcgacg aggacggctt cctcaactcc tactgcaata cgatcccgac
     2461 ggccgagggt ggcacccacg agcagggctt ccgcaccgcg ctcgccaagg ggctgcgcgc
     2521 ctacggggaa ctcacaaata gcaagaaggc ctcgcagatc acggccgacg acgtgttcgg
     2581 cacggccgcg gggatgctct ccgtgttcat ccgcgagccg gagttccagg gccagaccaa
     2641 ggacaagctc gcgaccgtcg aggcggggcg gatcgtcgag gcggccgtac gcgacgcctt
     2701 cgaccattgg ctcgccgaca gtccgcagca ggcgacgcgc ttgctcgaat ggacgatcga
     2761 gcagtccgag gagcgcctca agcggcggcg cgaaaaggaa gtgagccgcc agtcggcgac
     2821 gcgcaagctc cgtctcccgg ggaagctctg ggactgctcg tcgacgtcag gtgacaccga
     2881 gctcttcatc gtcgagggcg actcggccgg cggctccgcc aagcaggcgc gcgatccgaa
     2941 aacgcaagct gtgctgccgc tgaggggcaa gatcttaaac gtcgcaagcg ccacctcgca
     3001 aaagcttgcc gccaaccagc agctgaccga tctcctgcag gcgctgggtg tcggcacggg
     3061 ctcgaagtat cgcgccgagg atctgcgtta tgaccgcatc atcgtcatga cggacgccga
     3121 cgtggacggc gcccacattg cctcgctgct gatcacgttc ttctatcagg agatgcccga
     3181 gatgatcgaa ggcgggcacc tctatctggc ggtgccgccg ctcttcaagc tcactcacgg
     3241 ggcgaaaacg atctacgccc gcgacgatgc cgagcggctg cggcttctca agtccgagtt
     3301 caacgccaac gccaaggtcg aagtgacgcg cttcaaaggt ctcggggaga tgaacgcgtc
     3361 ccacttgaag gagacgacca tggacccgcg gcggcgtacc ctcctcaagg tcgagatcgt
     3421 cgacgaccgc gcccacctct cgcaggccgt gagcgacctc atgggcacca agcccgaggc
     3481 acgctacaag ttcatccagg agcgggcggc attcgccaag gacctcgaca tctgagccga
     3541 gcctgacgcc ggagcgacgc ccgcggcccg cctgagtgaa ctgggagagg catgatgagc
     3601 ttccggagac gagcgtcagc tgcggcgctg ctcgtagcgc tgccggcgat cgggaccgtg
     3661 gttgcccagg acggcgccca ggttgcgcgt ggccgccagc tgctcgaggc ccagtgcgcg
     3721 cgctgtcatg ccgtcggcgc aagcgacgca agccccgtca agcaggcgcc ggcgctgcgc
     3781 cagctgcgtc gccggtatcc ggtcgacgcg ctcgccgagg cactcgccga aggcatcatc
     3841 acaggccatc cgggcatgcc cgagttcgcc tttcaggcaa atgatgttgc ggccatcatc
     3901 gcctacctca aatcgcttcc cgatccgcgc tgacatcagc ctcacgtccg cgccgcgtcg
     3961 atgcctgcgc gatatgcagc ccggctgtgc accagctcgc gtcgctccac ctcatcccga
     4021 tcttgcgggc ttgcctggag cagctattct ccccgcatac gacaaaagtc ggatgccgag
     4081 ggagagcgcg atgaaggccg cagtgctgca cgaggtgaac aagccgctcg tgatcgagga
     4141 cgtggacgtc aagaagcccg gtccgcgcga ggtgctaatc cggacggcgt tcgcggggct
     4201 ctgccactcc gatctgcact tcatggaagg tctctacccg catccgctgc cggtcgtgct
     4261 cgggcatgaa tcagccggcg tggtggaagc cgtcggctcg gaagtgcatt acgtccagaa
     4321 gggcgaccac gtcatcacct gcctctcggt gttctgcggc acctgcgaga actgcacctc
     4381 gggccggcca gtgatctgca cggacacctc tgtgaagatg ctgcccggcc aggcccagcg
     4441 cctctcgctg aagggcaacc acgtaaacac gttcacgaac ctctcgtcct atgccgagat
     4501 gatgctcgtc cacgagaacg cgatcgtgaa gatcgacaag gactttccgc tcgaccgcgc
     4561 tgcgctgatc ggctgcggtg tgatcacggg cttcggcgcg gcggtgaata cggcgaaggt
     4621 ggccccgggc gagacggtcg cggtcatcgg ctgcggcggc gtcggcatgg ccgccgtcaa
     4681 tggagcggct gttgcgggcg cgggacgcat cttcgccgtc gacaccaatc cggcgaagct
     4741 gcagatggcc gcgaagatcg gcgcgaccga cctcatcaat ccgcgcgacg gtgatccggt
     4801 ggagcgcatc cgcgcggccc tcggcggcaa accgagcgac ggcgtgcacc acgcgatcga
     4861 gtgcctcggc ctcaaggtga cggccgagca gtgcttccag atgctccgcc acggcggcac
     4921 ggcgaccatt gtcggcatga tcccgttcgg gaccaagatc gagctgcacg gcttcgattt
     4981 cctgcgcgag aagaaggtgc agggctcgtc catgggctcg aaccgcttcc gcaccgacat
     5041 gccgcgcctc atggagttct atcgccgcgg caagctgcat ctcgacgact tcgtctccgc
     5101 ccacatcaag cttggcgaga tcaacgaagg tttcgcgagc atgaaggctg gcaaggtgat
     5161 ccgctcgatc atcgatttcg gggtcgtgaa agcacggcca tgattgcgct ataggcccca
     5221 cccgaggtca gtcccggttg gcgccggcgc gctggtgtgc catggtgccc catcccttcc
     5281 gagaagcgac gtcctgcccg aacgggtgac gcgaaacaag cgaggcctga agcatgcgat
     5341 acgaggcgcc ggattctctc gaggcggcgg caaagctgct ctcgtccgag agtggcgagg
     5401 cacgggtatt ggcgggcggg acggacctgc tcgtccagat gcgagccgac gtcgtccacc
     5461 ccgtcctggt cgtcgacatc aagcggatcg cggagacgcg gcggatcgtc gaagagggcg
     5521 gcaacttccg gatcggcgcg gccgtgaccg gcgccgaact caaggagcat gctgccctca
     5581 aggcggcact gccgggcctg gtcgaagccg caaacctgat cggctcgacg caggtgcagg
     5641 gccgcgccac gatgggcggc aatctgtgca acggttcgcc ggccgccgac agtgtcccgg
     5701 cgctgatcgc ggccggagca aaggcgtcga tcgtcggtcc cggcggg
//
LOCUS       DAORNH010000297         5695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10475_length_5695_cov_2.727482, whole genome shotgun sequence.
ACCESSION   DAORNH010000297 DAORNH010000000
VERSION     DAORNH010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10475_length_5695_cov_2.727482"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK264_21495"
     CDS             <1..245
                     /locus_tag="PK264_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78479.1"
                     /translation="AMSYVLRGEARVRLGEADAARADFTAAIRIMPANARALADRARL
                     AKAAGDCAGAIADFDSAIRITPNDASLIAERRLCVP"
     gene            559..1335
                     /locus_tag="PK264_21500"
     CDS             559..1335
                     /locus_tag="PK264_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78480.1"
                     /translation="MDLELKGRRVLVTGASKGIGRAVGEAFAAEGCDLVLVSRTAEAL
                     AKVADDIRARHQVRIETHAADLSQDKAREETAVRFSGVDILVNNAGAIPGGSLLDFTM
                     ARWQEVWALKVMGYIHMTQLHLRAMMERKRGTIINIVGMAGPAPRWDYICGSVGNAAL
                     NTFTKALGGKSHEFGVRVFGINPAATRTDRIFTLARQRAKTQFGDESRWEETLGAMPF
                     GRLKEPEEVAALAVMLASPKVTYLSGTMIDMDGGGQYRGK"
     gene            1340..1678
                     /locus_tag="PK264_21505"
     CDS             1340..1678
                     /locus_tag="PK264_21505"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRD78481.1"
                     /translation="MIISKGNAPHYVWGGDCDGWRLADTAGLSVIHERMPAGRAETRH
                     HHAQARQLFYVLAGELTMERDSATDVARAGEAIEIAPGERHRAVNASAADVEFLVISA
                     PTTRGDRVEG"
     gene            1744..2577
                     /locus_tag="PK264_21510"
     CDS             1744..2577
                     /locus_tag="PK264_21510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HRD78482.1"
                     /translation="MIFFRRKPVVPVVRLAGTIGMGAPLRSGLTLAGVAGALERAFTM
                     SRLPTVAIVVNSPGGSPVQSSLIFRRIRQLADEHGKRVYAFAEDVAASGGYYIAVAGD
                     EIYADPSSVLGSIGVISAGFGFDKAIEKLGVERRVHTAGRSKATLDPFSPEKPEDVER
                     LKAVQRDIHDAFIAVVKERRGARLKGTDDELFSGAFWSGRKSVEIGLADGIADLRATM
                     RGLHGKDVQLRMVSTETGGWLSRRLRRVPLTQANPVVAGLAEEALAALEERQLWSRFG
                     L"
     gene            complement(2593..3414)
                     /locus_tag="PK264_21515"
     CDS             complement(2593..3414)
                     /locus_tag="PK264_21515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HRD78483.1"
                     /translation="MAKMQTGTVRSLTATIGTAAMIMLAGPVAAQDAKPQITGNFGVA
                     SEYVFRGFSQTAEKWAVQGGIDMTYKWFYAGIWGSNLDFGRDVTPAGVSRPVASTEVD
                     FYAGIKPVFGRVTFDIGIIYYSYPRALDGALQVSRELDYIELKLGASGEIWKDGTLGL
                     TAFYSPEYTNAQGEVWTFELAFAQVLPKMRDITPTFSALLGYQIGDDARYIAITGNGA
                     DDYLYWNVGLTLGFGDRFSLDLRYWDTNVSNTGGFCSGQIFQCDERVVGSAKVTF"
     gene            complement(3631..4086)
                     /locus_tag="PK264_21520"
     CDS             complement(3631..4086)
                     /locus_tag="PK264_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD78484.1"
                     /translation="MSKIIGKLLQPYWRLTRGLTLGAQGVVLDPDRRVLLIRHTYRKG
                     WFFPGGGVEAGETVETALERELAEEAGIEIAGPPRLFGLYANFRAFPRDHIALFIVDA
                     WRQPAVPPPNAEIAEQGFFARGALPDDISPGTLRRIGEVLDSASRSRVW"
     gene            complement(4147..4359)
                     /locus_tag="PK264_21525"
     CDS             complement(4147..4359)
                     /locus_tag="PK264_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HRD78485.1"
                     /translation="MRLAVTAAIAAIAGSTFISLATAQTPPKPRTAESIQCSAEADQK
                     GLKGKERQKFRKACIAAAKKKKGSAA"
     gene            4656..>5299
                     /locus_tag="PK264_21530"
     CDS             4656..>5299
                     /locus_tag="PK264_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRD78486.1"
                     /translation="MRTLAGQDKAGLATALASVGVPEKQIRMRVGQLWRWLYVHGATS
                     FDQLTDVSKELRQKLAATHTLTRPEIVAEQISIDGTRKWLLRLERLGHEARAPEIECV
                     YIPETDRGTLCISSQVGCTLTCSFCHTGTQKLVRNLEVHEIVGQILLARDRIGDWPGA
                     NPPDDGGLLPYTERKITNIVLMGMGEPLYNFDNVKAAMEVASDGEGLAVSKRRIT"
     assembly_gap    5300..5309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5310..>5695
                     /locus_tag="PK264_21535"
     CDS             <5310..>5695
                     /locus_tag="PK264_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRD78487.1"
                     /translation="STSGVVPQITRWGAEAGTMLAISLHATRDDLRNELVPINKRYPI
                     AELLAACRAYPGLSNARRITFEYVMLKGVNDSIADARALVKLLAGIPAKINLIPFNPW
                     PGTKYECSDWDQIERFADVVNRAGYA"
BASE COUNT         1000 a         1874 c         1888 g          923 t
ORIGIN      
        1 acgccatgtc ctacgtgctg cggggagagg cgcgcgtgcg cctcggcgaa gctgacgccg
       61 cgcgcgcgga tttcacggcc gccatccgga tcatgccggc caacgcgcgc gccctcgccg
      121 accgggcccg cctcgcaaag gcggccggcg actgcgcggg cgcgatcgcc gatttcgatt
      181 ccgccattcg catcacgccc aacgatgcca gcctgatcgc cgagcggcga ctgtgcgtgc
      241 cgtaagtacg gcctaagcct tgcgagccgg aacgagctgc tggtgccgga ccgaaggtgg
      301 aattgccgtg tcaagcaagg gagtgcctgc ggggccggat tcgctcctcg ggtgatggcg
      361 agaggccttc cgcctctcgc gctctcagac cgagagctgg cccctgggcc tgcctttcag
      421 agagccactt gaaagagctg gcctgtcatg actgagccat gctcccatgg acagcacggt
      481 cgtggcgcta tggccccgag actgcgtagt agtcggccca gaggccggat ctaagttcaa
      541 ccgacaggag tgccacagat ggatctcgag ctgaagggac gccgcgtcct ggtcaccggc
      601 gcgtcaaagg gcatcggccg cgcggttggc gaggcctttg ccgccgaggg ctgcgatctc
      661 gtgctcgtct cgcggacggc cgaggcgctc gccaaggtcg ccgacgacat ccgcgcgcgc
      721 caccaggtgc gcatcgagac tcacgccgcc gatctctcgc aggacaaggc gcgcgaggag
      781 accgccgtcc gcttctcggg cgtcgacatc ctcgtcaaca atgcgggcgc gatcccgggc
      841 ggcagcctcc tcgacttcac gatggcgcgc tggcaggagg tttgggcgct gaaggtgatg
      901 ggctacatcc acatgacgca gctccacttg cgggcgatga tggagcgcaa gcgtggcacg
      961 atcatcaaca tcgtcggcat ggcgggaccg gcaccgcgat gggactacat ctgcggctcc
     1021 gtcggcaatg ccgcgctcaa caccttcacg aaggcgctcg gcggcaagag ccacgagttc
     1081 ggcgtgcgcg tcttcggcat caatccggcg gcgacccgga cggaccgcat cttcactctc
     1141 gcccggcagc gcgcgaagac ccagttcggc gacgagagcc gctgggagga gacgctcggc
     1201 gctatgccgt tcgggcgcct gaaggagccc gaggaggttg cagccctcgc cgtgatgctc
     1261 gcctcgccca aagtgaccta cctctccggt accatgatcg acatggacgg cggcgggcag
     1321 tatcgcggaa agtgacaccg tgatcatctc gaagggaaat gcgccgcact acgtctgggg
     1381 cggcgactgc gacggctggc ggctcgccga tacagcgggc ctgtccgtca ttcatgagcg
     1441 catgccggcg ggccgcgccg agacgcggca ccaccacgca caggcgcgac aattgttcta
     1501 cgtactggcg ggcgaactca cgatggagcg cgatagcgcg acggacgtcg cgcgggccgg
     1561 agaggcgata gagattgcac cgggcgagcg ccaccgcgcc gtgaacgcct cggcagccga
     1621 cgtcgagttc ctggtgatct cggcaccgac gacacgcggt gatcgtgtcg agggctgaac
     1681 ggacttgcag cgctcgcccg cgcatcctat gtggcagcgc acgccaacgc ccgggaccac
     1741 cagatgatct tctttcgccg caagcccgtc gtccccgtcg tgcgcctggc ggggaccatc
     1801 ggcatgggag caccgctccg atccggattg acgctcgcgg gcgtcgccgg cgcgctcgag
     1861 cgggccttca cgatgtcgcg gttgccgacg gtggcgatcg tcgtcaactc cccgggcggc
     1921 tcgccggtgc agtcgagcct gatcttccgc cgcatccgcc agctcgccga cgagcatggg
     1981 aagcgcgtct acgccttcgc cgaggacgtc gcggcctcgg gcggctacta catcgccgtc
     2041 gcgggcgacg agatctatgc cgatccctcc tcggtgctcg gctcgatcgg cgtcatctcg
     2101 gcaggtttcg gcttcgacaa ggcgatcgag aagctcggcg tcgagcggcg cgtgcacacc
     2161 gccggccgct ccaaggcgac gctcgatccc ttcagccccg agaagcccga ggatgtcgag
     2221 cggctgaagg cggtgcaacg cgacatccac gacgccttca tcgccgtcgt caaggagcgg
     2281 cgcggcgcca ggctcaaagg cacggacgac gagctcttct cgggcgcgtt ctggtctggg
     2341 cgcaagtccg tcgaaatcgg gctcgcagac ggcatcgccg acctgcgcgc cacgatgcgc
     2401 ggcctgcacg gcaaggacgt gcaactccgc atggtatcga cggagaccgg aggatggctg
     2461 tcccgccggc tccgacgcgt gccgcttacg caagcgaacc ccgtcgtcgc ggggctcgcc
     2521 gaggaggctt tggcggcgct cgaggagcgc cagctctggt cgagattcgg cctctgagct
     2581 ctcagcgaga gatcagaacg tgaccttcgc ggaaccgacg acgcgctcgt cgcactggaa
     2641 gatctgaccc gagcagaagc cgcccgtgtt cgagacgttg gtgtcccagt agcgcaggtc
     2701 gaggctgaag cggtcgccga agccgagcgt caggccgacg ttccagtaga ggtagtcgtc
     2761 ggcgccgttg cctgtgatcg cgatgtagcg ggcatcgtcg ccgatctggt agccgaggag
     2821 cgcgctgaag gtcggcgtga tgtcgcgcat cttcggcagc acctgcgcga aagcgagctc
     2881 gaacgtccaa acttcgccct gcgcgttggt gtactcgggc gagtagaagg ccgtgagacc
     2941 gagcgtgcca tccttccaga tctcgccgct cgcgccgagc ttgagttcga tgtagtcgag
     3001 ctcgcggctc acctgcagcg cgccgtcgag ggctcgcggg taggaatagt agatgatgcc
     3061 gatgtcgaag gtaacgcggc cgaacaccgg cttgatgccg gcgtagaaat cgacctccgt
     3121 cgaggcgacg gggcgcgaaa cgccagcggg cgtcacgtca cgtccgaagt cgaggttcga
     3181 accccagatg ccggcataga accacttgta cgtcatgtcg atgccgccct ggacggccca
     3241 tttctccgcc gtctgcgaga agccgcggaa cacgtactcg ctcgccactc cgaaattgcc
     3301 ggtgatctgc ggcttcgcat cctgtgccgc cacaggcccc gccagcatga tcatcgccgc
     3361 cgtaccgatc gtcgccgtca gcgatcgcac tgtccccgtc tgcatcttcg ccatcccatc
     3421 gcttcgttgc gcaccctgcg ctgtggcgac aagaaagagg gaaaaggggg gctgtcgcaa
     3481 tggcagattg gcgctcggcg gctcgattga cgagccctgt cgcgatcccg caacatattg
     3541 caaaacatga ctatttctca atcacactgc tgcctgaaac ggcgccgatt tctgcacata
     3601 ttttgatcgt ggcgcccaat tggaatgcac tcaccacacg cgcgagcgcg atgcgctgtc
     3661 gaggacctcc ccgatccggc gcagagttcc ggggctgatg tcgtcgggaa gcgctccgcg
     3721 agcgaagaat ccttgctcgg caatctccgc attgggcggt ggcactgccg gctgccgcca
     3781 agcgtcgaca ataaagagcg ctatgtgatc gcggggaaag gctcgaaaat tggcgtagag
     3841 gccgaacaat cgcggcggcc cggcgatctc gattccggct tcctcggcaa gctcccgctc
     3901 gagcgcggtc tcgacggtct cgccggcctc gacgccgccg ccgggaaaga accagccctt
     3961 gcggtaggtg tgccggatga ggagcacacg gcggtccggg tcgagcacca caccctgcgc
     4021 accgagcgtg agcccgcgtg taagccgcca atagggttgc agaagcttgc cgatgatctt
     4081 ggacattgcg aaggagcgtc cgcgccggcc acccgcgacg ggcaggcagc gcggacacgg
     4141 ttccgttcag gcagctgaac cctttttctt cttcgcggca gcgatgcagg ccttgcggaa
     4201 cttctggcgc tccttgccct tcaagccctt ctgatcggcc tcggcggagc actggatcga
     4261 ctcggctgtg cgcggcttcg gcggcgtctg ggcagtggcg aggctgatga acgtcgagcc
     4321 ggcaattgcg gcgatcgcgg cagtgactgc aagtctcatg ggtgcactcc tacaggcgat
     4381 tgacgagcgg cgtcgccacg gacggccggt ccggggcgca gggattggca ccggacgaat
     4441 cttcatacgc cctatgatcc ctcgtgaaca tgggacagaa cgacggggga gagaacgaga
     4501 gtatcaggag agacatgggc gaaagacatg ggcttcccca gcgcggctgg ggccgctaga
     4561 aggggcccga acccccgtct cgaggcttcg gctgacgcgc tatggtcctg gttgtcgaca
     4621 aatccgcaga actcctgccc gccgcgccgc gacgcatgcg gacgcttgcc ggacaagaca
     4681 aggcgggcct tgccacggcg ctcgcctccg tgggcgtccc ggagaagcag atccgcatgc
     4741 gagtcggcca gctctggcgc tggctctatg tccatggagc gacctcattc gaccagctga
     4801 ctgatgtctc gaaggagctg cgccagaagc tcgccgcgac acacacgctt acccggccgg
     4861 agatcgtcgc cgaacagatc tcgatcgacg gcacgcgcaa gtggctgctg cgcctcgagc
     4921 gactcgggca cgaggcccgc gcaccggaga tcgagtgcgt ctatataccg gagactgacc
     4981 gcggcacgct ctgcatctcc tcgcaggtcg gctgcacgct cacctgctcg ttctgtcata
     5041 ccggcacgca gaaactcgtg cgcaacctcg aggtgcacga gatcgtcgga cagatcctgc
     5101 tcgcccgcga ccggatcggc gattggccgg gcgcgaaccc gcccgacgat ggcgggctgc
     5161 tcccctacac cgagcgcaag atcaccaaca tcgtgctcat ggggatgggc gagccgctct
     5221 acaatttcga caacgtgaaa gcggccatgg aggtcgcctc ggacggcgaa ggcctggccg
     5281 tctcgaagcg gcggatcacn nnnnnnnnnt gtcgacctcg ggcgtcgtcc cgcagatcac
     5341 tcgctggggc gcggaagcag gaacgatgct tgcgatatcg ctgcatgcaa cgcgcgatga
     5401 tctgcgaaac gagctcgtgc cgatcaacaa gcgctatccg atcgccgagc tgctcgccgc
     5461 ctgccgcgcc tatccggggc tttccaatgc gcggcggatc accttcgagt acgtgatgct
     5521 gaagggcgtg aacgacagca ttgcggacgc gcgcgcactc gtgaagctgc tcgccggcat
     5581 tccggccaag atcaacctca tccccttcaa tccgtggcca gggacgaaat acgagtgctc
     5641 cgactgggat cagatcgagc gctttgccga cgttgtcaat cgcgcgggct atgcg
//
LOCUS       DAORNH010000298         5694 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10482_length_5694_cov_2.911154, whole genome shotgun sequence.
ACCESSION   DAORNH010000298 DAORNH010000000
VERSION     DAORNH010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10482_length_5694_cov_2.911154"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PK264_21540"
     CDS             complement(<1..632)
                     /locus_tag="PK264_21540"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HRD78488.1"
                     /translation="MSKMLFTPYRMRGLELPNRLVVSPMCQYSAENGSMTDWHVMHLG
                     TLAQSGAGLLIVEATAVEPEGRITHGCTGLYSDDNERAMKRVIDAVRRYGQAKIGIQI
                     GHAGRKASSKRPWDAREMNEPVEGGWTTRGPSAVPFKAGWNMPHEMTLAEIAELKQKF
                     VASTVRANRIGYDMIELHAAHGYLLHQFMSPLSNKRTDKLIVRSRHLKKKR"
     gene            828..1322
                     /locus_tag="PK264_21545"
     CDS             828..1322
                     /locus_tag="PK264_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78489.1"
                     /translation="MAQRWANPAALTAIGLLLGACAQAAPGYEPPTARNAKRLAQPDP
                     GPPGVVTPAGYVLSDSEKRYDCKKLTGIIQIKILQLREPASRPVPSAIAKTGPAIGGP
                     LFGSKAPDPAEERATEIARLRAYNTALGEKKCRTFDIDAELTKAAAAPVPRTSAPIAT
                     PTKN"
     gene            1567..2721
                     /locus_tag="PK264_21550"
     CDS             1567..2721
                     /locus_tag="PK264_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD78490.1"
                     /translation="MLRRGLSTVLLLVGAAVVAGGALYGATRYVRETSLLSATAPDRG
                     KADPKARTKGKAGAAVAVEAVKAIGSKTTVDIKAIGSLRSDESVQIAAEISGRIQSIN
                     FAEGGDVKAGEVLVKLDDSLAEAEVADAKARFDLAEANNQRAKRLSRTGSVTEKAVDE
                     ATTTLEIARAAVELQRVKLARHVITAPFAGRVGIRKASPGGFISAGTAIVNLEKIDVL
                     KVDFKLPEVFLAAVGVGQVVEIQVDALPGRSFAGEIYAIDPQVDVNGRAMALRARIAN
                     PELVLRPGLFARILVKGKDVRDAVRVPEAAIVSRGGETYVFRIDANIVSEVKVRLGER
                     RGADVEILEGLSANQLVVSAGQMKLRNGLPVEIVAPASEPATTPSRKSPG"
     gene            2718..>5694
                     /locus_tag="PK264_21555"
     CDS             2718..>5694
                     /locus_tag="PK264_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD78491.1"
                     /translation="MSLFEFCIRRPVFSTVLSLAIVLLGLMSYSRLTVREYPNVDEPV
                     VSVATSYPGASAAIVESQVTQVLEGSIAGISGIDVLESTSRAETSRITVRFRPDVDAD
                     VAASDVRDRVSRVRRRLPNEVQEPTIAKVEADAQAIMFLVFTAEGMSALEITDYVNRY
                     VVDRLKNLSGVADVTIFGERRYAMRIWIDRERLAAFSLTVQDVESALRAQNVELPSGR
                     IESKDREFAVMARTGLVTTDQFANIIIKIADGHQVKLGEVARIELGAQDARRDSRFDG
                     KPAIAVGIIKQAVANPLDVSKAVRDSLPLINASLPKGLTVTIGNDSSVFIDRSIKAVY
                     QTIAEAIALVVIVILLFLRSGRASIIPIVTIPISLIGTFALLNAMGFTINTLTLLAMV
                     LAIGLVVDDAIVVLENINRRIEHGMRPIPAAIEGTKEIGFAVIAMTLTLAAVYAPIAF
                     APGRTGRLFLEFALTLAGAVIVSGFIALTLTPMMCAKLLRHNPNPGRIASFIERAFMR
                     LERGYRRLLTRTFNVRPLVLAVGLAVALLGAQVYVSLKSELAPVEDRGLLQIRGTGPE
                     GATLAYTSRYSSQVAEILAKVPEVESVLIINGSPEVSRFNTVGRLADWSLRKRSQQLI
                     NAEINPKLRRIAGLNVSAFNPGSFGVRSSSRPVEFVLQTSGTYEELQSYVDLMMERIR
                     DAGILESIESDLQLNQPEFRIDIDRAKVADLGLDVTIVGRTLETLLGGRQVTRFERAG
                     EQYDVIVQLAADERASPRTLSTIFLRSPSGEMIQLANIVRVREAVAPKELKRFNQLRA
                     VTLSANLVPGVSLGEGIAFMEATAKSVLPSTVLTDVGGQSREFRAAGQSLVMVFLLAL
                     AFIYLVLAAQFESFRDPLIILVTVPLSMTGALSALWLTDGTLNVYSQIGLVTLVGLIT
                     KHGILIVEFANQLQERGYARLNAAIKAAGLRLRPILMTTAAMVLGAIPLAMATGAGAE
                     SRRQIGWVIVGGMSIGTLLT"
BASE COUNT          993 a         1800 c         1841 g         1060 t
ORIGIN      
        1 cgcttcttct tgaggtggcg cgaacgcacg atgagcttgt cggtgcgctt gttggagagc
       61 ggcgacatga actgatgcaa caaatagccg tgggcggcat gcagctcgat catgtcgtag
      121 ccgatgcggt tcgcccgcac ggtcgaggca acgaacttct gcttgagctc cgcgatctcg
      181 gcgagcgtca tctcgtgcgg catgttccag ccggccttga acggcacagc cgagggcccg
      241 cgcgtcgtcc agccgccctc gactggctcg ttcatctcgc gcgcgtccca ggggcgcttc
      301 gaggaggcct tgcgaccggc atgaccgatc tggatgccga tcttcgcctg gccgtagcgg
      361 cggacggcgt cgatcacgcg cttcatggcg cgctcgttgt cgtcggagta gaggccggtg
      421 caaccgtgcg tgatgcggcc ctcgggctcg accgcggtcg cctcgacgat gaggaggccg
      481 gcgccggatt gcgccagcgt gccgagatgc atgacgtgcc agtcggtcat ggagccgttc
      541 tcggccgagt actggcacat cggcgagacg acgaggcggt tcgggagctc gaggccgcgc
      601 atgcggtatg gcgtgaacaa cattttcgac atcgtggacc tatcgttgat gatcgcacat
      661 cgttgacccg tcgagggttc cacgcagcgc tagcatggcc cgaaaaggcc gtcatccgtg
      721 gatttggcga acggttggcg gccatgcacc cggatcaaag tccagcgccg gccctcacct
      781 ttgccatggt cgcgtgctat ccgggccggc tagtcgaagg agacgacatg gcacagcgat
      841 gggcaaaccc agctgctctc accgcgatcg gcctgctgct cggcgcctgc gcgcaggccg
      901 cacccggcta tgagccgccg acggctcgca acgcgaagcg gctcgcgcag cctgacccgg
      961 gcccgcccgg cgtcgtcacg cctgcgggct acgtgctctc ggattccgag aagcgctacg
     1021 actgcaagaa gctcaccggc atcatccaga tcaagatcct ccagctccgc gagcccgcga
     1081 gccgtccggt gccgtctgcg atcgcgaaaa ccggaccggc gatcggcggc ccgctgttcg
     1141 gctcgaaggc gcccgatccg gccgaggagc gcgcgaccga gatcgcccgt ctcagagcct
     1201 acaatacggc tctcggcgag aagaagtgcc ggacgttcga catcgacgcc gagctaacca
     1261 aggccgccgc agcaccggtt ccgaggacct cggcgccaat tgcgacaccg acgaagaact
     1321 agggcggaac tacggatttc gatcccgctg ggcggttgca tcgtgcacgc cagacatatt
     1381 gcgtgcaaaa tggtggtgcg cctggagtag gctcgttgcg cggggcgcgc ggtgcccatg
     1441 ccgcccgaac tcggcaattg tgcgacggat cgcaatatct cgagcgtttt tgggcatgat
     1501 ggcacgtgcg gccagtgatg acgggaccgt gattgggcgc acgtgccgag tgagtgaggt
     1561 gcagccatgc ttcgtcgtgg cctttcgacg gtacttttat tggtcggcgc cgcggtagtg
     1621 gcgggaggcg cactttacgg tgcgacgcgt tacgtccgcg aaacttcgct cttgtcggcg
     1681 acagcgcccg atcgcggcaa ggcggatccg aaggcccgga ccaagggcaa ggcgggcgcg
     1741 gccgtcgctg tcgaggccgt caaggcgatc ggcagcaaga cgacggtcga catcaaagcg
     1801 attggcagcc tcagatcgga cgaatccgtt cagatcgccg ccgagatctc cggacgcatc
     1861 caatccatca attttgccga aggcggtgat gtcaaagccg gcgaggtgct cgtcaagctc
     1921 gacgattcgc ttgccgaagc cgaggtcgcc gatgccaagg cgcgcttcga tctcgccgaa
     1981 gccaacaacc agcgcgcgaa gcggttgtcg cgcaccggga gcgtcaccga gaaagctgtc
     2041 gatgaggcca caaccacact cgagatcgca cgtgccgcag tcgaactgca gcgcgtcaaa
     2101 cttgcgcgac acgtgatcac ggcacctttc gccgggcgtg tcggaattcg gaaagcctcg
     2161 cccggaggtt tcatctcagc cggcacagca atcgtcaatc tcgagaagat cgatgttctg
     2221 aaggtcgatt tcaaattgcc cgaagtattc cttgcagcag taggtgtcgg ccaggtcgtc
     2281 gaaatccagg tggacgctct gccggggcgg agtttcgcag gcgagatcta cgccatcgat
     2341 ccgcaggtcg acgtcaatgg ccgtgccatg gcgctgcgcg cccgcatcgc caatcccgag
     2401 ttagttctgc gccccgggct cttcgcccgc attctcgtca agggcaagga tgtgcgcgat
     2461 gccgtccggg ttcccgaggc agcaatcgtc tcgcgaggcg gcgagacata cgtgttccgt
     2521 atcgacgcga acatcgtgag cgaagtgaag gtcaggctcg gagagcggcg aggcgcggat
     2581 gtcgaaattc tcgaaggcct cagcgccaat cagttggtcg tgagcgccgg ccagatgaag
     2641 ttgcggaatg ggctcccggt cgagattgtc gccccggcaa gtgagcccgc aacaactcct
     2701 tctcggaagt cgccaggatg agcctctttg agttctgcat caggcgtccc gttttctcga
     2761 cggtgctgag cctcgcgatc gtgctgctcg gactgatgtc ctacagccgg ctcaccgtgc
     2821 gcgaatatcc gaatgtcgac gagccggtgg tctcggttgc gacgagctat cccggagctt
     2881 cggccgccat cgtcgagagt caagtcacgc aggtccttga agggtcgatt gccggtatct
     2941 cggggatcga tgtgctcgaa tcgacgagcc gcgccgaaac gagccgcatt actgtccggt
     3001 tccggcccga cgtcgacgcc gacgtcgcag ccagcgacgt tcgcgaccgt gtgagccggg
     3061 tgcgccggcg cctccccaac gaggtgcagg agccgaccat tgccaaggtc gaggccgatg
     3121 cccaggcgat catgttcctg gtcttcacgg ccgagggcat gtcggcgctc gagatcaccg
     3181 actacgtcaa ccgctatgtg gtagatcgcc tgaagaacct gtcgggagtt gccgatgtca
     3241 cgatcttcgg cgagcgccgc tatgctatgc ggatctggat cgaccgggag cggctcgcgg
     3301 ccttcagttt gaccgtccag gacgtcgaga gcgcgcttcg ggcccagaac gtcgagctgc
     3361 catccgggcg catcgagagc aaggatcgcg agttcgcggt gatggcccgg acaggcctcg
     3421 tcaccacgga ccagttcgcc aacatcatca tcaagatcgc cgacggtcac caggtgaagc
     3481 ttggcgaggt tgcgcgcatc gagcttggcg cgcaggatgc gcgccgcgac agtcgcttcg
     3541 acggcaagcc ggcaatcgcc gtcggcatca tcaagcaggc ggtcgccaac ccgctcgatg
     3601 tctcgaaggc cgttcgcgat tcgctgccgc tgatcaacgc gtcgctgccg aaaggcctga
     3661 ccgtcacgat cggcaacgac agctccgtgt tcatcgaccg atcgatcaag gcggtctatc
     3721 agacgatcgc tgaggccatt gctctggtcg tgatcgtgat cctgctgttt ttgagatccg
     3781 gtcgagcttc tatcattccg atcgtgacca tcccgatctc gctgatcggc accttcgcgc
     3841 ttctgaatgc catgggcttc acgatcaata ccttgaccct cctcgccatg gtgctcgcca
     3901 tcggcctcgt ggtcgacgac gcgatcgtgg tgctcgagaa catcaatcgg cggatcgaac
     3961 acggcatgcg gccgattccc gccgcgatcg agggaacgaa ggagatcggc tttgccgtga
     4021 tcgccatgac gttgacgctc gcggcagtct atgcgccgat cgcgttcgcg cctggccgga
     4081 cagggcgatt gttcctcgaa ttcgcattga cgctggctgg cgccgtcatc gtatcgggct
     4141 ttattgccct gaccttgacc ccgatgatgt gcgcaaaact gctgcgccac aatccgaatc
     4201 ccggtcgaat tgcgtcgttc atcgagcggg cattcatgcg gctcgagcgc ggctatcgcc
     4261 ggctgttgac ccgcacgttc aacgtgcgcc cgctggttct cgctgtcggc ctcgcggtcg
     4321 cgctccttgg cgcgcaagtc tacgtgagcc tcaagagcga gctggcgccg gtcgaggatc
     4381 gcgggcttct ccagatccgc ggcacgggac ctgaaggagc tacgctcgcc tatacgagcc
     4441 gctacagcag tcaggtcgcc gagatcctcg ccaaggttcc tgaggtggaa tcggttctga
     4501 tcatcaacgg ctcacccgag gtatcgcgct tcaatacggt tgggcggctc gcggactgga
     4561 gcctccggaa gcgctcgcag caactcatca acgcggagat caatccgaag ctgcgccgta
     4621 ttgcggggct caacgtatcg gccttcaatc ccgggtcgtt cggcgtccgg tcgtcgtcac
     4681 ggccggtcga attcgtcctg cagacgtcgg gcacctacga ggagttgcag tcctacgtcg
     4741 acctcatgat ggagcggatc cgggacgccg gaattctcga aagcatcgaa tctgatctgc
     4801 agctcaacca gcccgagttc cggatcgata tcgatcgtgc caaggtcgcc gatctcggac
     4861 tcgacgtgac gatcgtcggc cgcacgctcg agacgctgct cggcggtcgc caggtgacgc
     4921 ggttcgagcg agcgggcgag cagtatgatg tgatcgtcca gcttgcggcc gacgaacgcg
     4981 cttcgccccg cactctgtcg acgatctttc tgcgctcgcc gtcgggcgag atgatccagc
     5041 ttgccaacat cgtccgcgtg cgcgaggccg tggcgccgaa ggagttgaag cgcttcaatc
     5101 aattgcgcgc cgttacgctg tccgcgaacc tcgtgcccgg cgtctcgctc ggcgagggta
     5161 tcgctttcat ggaagctacg gcgaagagcg tgctcccgtc gaccgtgctg acggatgtcg
     5221 gcgggcaaag ccgcgaattc cgcgcggccg gccagagcct tgtcatggtg tttctgttgg
     5281 cactcgcgtt catctacctc gtgcttgccg cccagttcga gagcttccga gacccgctga
     5341 tcatcctggt caccgtgccg ctgtcgatga cgggcgcgct gagtgccctc tggctgaccg
     5401 atggcacgct caatgtctat tcgcagatcg gcctcgtgac gctcgtcggc ctcatcacca
     5461 agcacggcat tctgatcgtc gagttcgcga accagctgca ggagcgcggc tacgcgcgcc
     5521 tcaacgctgc gataaaggcg gcgggcctgc gtctgcgccc gatcctgatg accactgcgg
     5581 cgatggtgct cggggccatt ccgctcgcca tggcgaccgg cgcgggcgcg gagagccgcc
     5641 gccagatcgg ctgggtgatc gttggcggga tgagtatcgg cacgttgctc acgc
//
LOCUS       DAORNH010000299         5673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10538_length_5673_cov_2.118904, whole genome shotgun sequence.
ACCESSION   DAORNH010000299 DAORNH010000000
VERSION     DAORNH010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_5673_cov_2.118904"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>340
                     /locus_tag="PK264_21560"
     CDS             <1..>340
                     /locus_tag="PK264_21560"
                     /inference="COORDINATES: protein motif:HMM:NF024818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HRD78492.1"
                     /translation="IVRCNRAVIERLGLQYADVLGRSLPKLLLGDQSVPEGLFQAEIA
                     AVQFAALPGWFRVSTFPIQSDGRLTGLVHVVRDITHELAMATALRQSERQYRLLAENT
                     ADVVWVLSTET"
     assembly_gap    341..429
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <430..>807
                     /locus_tag="PK264_21565"
     CDS             <430..>807
                     /locus_tag="PK264_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRD78493.1"
                     /translation="TLDGVIAPESWPEMTTGLLGRLEAFRDGDPAARQRIDQILLLRR
                     DGTTVWTEVSTTLMLNEHQDCDLVGVTRDISVRKQAEDALAQQAYILDRLDEGVSVAD
                     QAGNLIFTNRSLDAMFGYAHSELA"
     assembly_gap    808..877
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1151..1237
                     /locus_tag="PK264_21570"
     CDS             1151..1237
                     /locus_tag="PK264_21570"
                     /inference="COORDINATES: protein motif:HMM:NF022612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78494.1"
                     /translation="MEAQISDTGSAWSARARPGHPDEPTEAS"
     gene            1280..2596
                     /gene="hisD"
                     /locus_tag="PK264_21575"
     CDS             1280..2596
                     /gene="hisD"
                     /locus_tag="PK264_21575"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRD78495.1"
                     /translation="MAKRLSTADPGFAAEFRRFLGEKREAAPDVDAAVSVIVADVRSR
                     GDAALIEFSRKFDRLELTRETLRIGADEIAAASKAVDPEMRTALTFARDRIKSHHARQ
                     LPKDDQYRDDLGVELGSRWTPIDSVGLYVPGGTASYPSSVLMNAVPAKVAGVARIAMV
                     VPTPEGRLNPLVLAAAELAGVEEIYRVGGAQAIAALAYGTETIAPVAKIVGPGNAYVA
                     AAKRQVFGAVGIDMVAGPSEVLIIADSDNDPEWIAADLLAQAEHDTAAQSILMTDDAA
                     FADRVEAAVEHQLAALPRGETARASWATFGAIILLGSLDEAPPLADRIAAEHLEIATR
                     APDALAARIRHAGAIFLGHCTPEVIGDYVAGSNHVLPTARSARFSSGLGVLDYMKRTS
                     MLRLTPQSFAALAPHAMRLAEAEGLGGHRRSVEIRLAPDASSRKST"
     gene            2593..3105
                     /locus_tag="PK264_21580"
     CDS             2593..3105
                     /locus_tag="PK264_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HRD78496.1"
                     /translation="MSTSMRDEPDPPHPDPKSRLVAITLDEQSILRSNPNIEHERQVA
                     IFDILDANHFAVEGRSDGPFKLDLSVVEERLALAITTEADAPVATHLLSLSPLRRIIK
                     DYFVVCDTYYEAIRSAPPSKIQAIDMGRRGLHDEGSRVLAERLKGKVELDHDTARRLF
                     TLICALRSKG"
     gene            3110..3559
                     /locus_tag="PK264_21585"
     CDS             3110..3559
                     /locus_tag="PK264_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRD78497.1"
                     /translation="MSQGVPGSVLFACTMNAVRSVMAAAILRHLAGKRVYVESAGVRA
                     GESDPFVAAVMSEIGIDVSQHHPRTIGELFDTNFDVVISLSPEAHHHAIEMTRTMAVG
                     VEYWPTLDATANRGGSREQILEGYRGVRDALFRRIKARFGFEGGPSV"
     assembly_gap    4123..4175
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4176..>4762
                     /locus_tag="PK264_21590"
     CDS             <4176..>4762
                     /locus_tag="PK264_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRD78498.1"
                     /translation="LLENLEHRGAVGADPLAGDGAGMLIQLPDAFLRAEMAAAKIKLP
                     KPGWYAVGQLFMPQDERLRAHCERVWARILREEGLVLLGWRSVPVDNSKLSEMVKETE
                     PVHRQIFVGRPEGMADEAEFERKLYLVRKVVSNALFYAYKERDIGHYTVSLSSRTLVY
                     KGMFLSFQLKAYFKDLSDPRMASTLALVHQRFSTNT"
     assembly_gap    4763..4801
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4802..>5673
                     /locus_tag="PK264_21595"
     CDS             <4802..>5673
                     /locus_tag="PK264_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007701046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRD78499.1"
                     /translation="INTLRGNVNWMAARQASVSSPLWGKDISKLWPISYEGQSDTACF
                     DNALEFLVMGGYSLAHAAMMLIPEAWAGNPLMDGKRRAFYEYHACLMEPWDGPAAMAF
                     TDGLQIGATLDRNGLRPARYLVTHDGLVVMSSESGVLPIEESRIAKKWRLQPGKMLLI
                     DMQKGRIISDDELKADLASRYPYEQWLKSTQIQVGDLPLPKKIAKKKSNVALLDLQQA
                     FGYTEESLKFLMSPMAHTGQEAVGSMGTDTPVSVLSSKPKLLHTYFKQNFAQVTNPPI
                     DSIREELVMSLVSF"
BASE COUNT          934 a         1820 c         1768 g          900 t
ORIGIN      
        1 gatcgtgcgc tgcaaccggg ccgtgatcga acggctgggc ctgcaatacg ccgatgtgtt
       61 ggggcgcagc ctgccaaaac tgctgctcgg cgatcagtcg gtgccggagg gcctcttcca
      121 ggcggagatc gcggcagtgc agtttgcggc cctgccggga tggttccggg tttccacgtt
      181 tccgatccag tccgacggcc ggctgaccgg cttggtgcac gtcgtgcggg acatcaccca
      241 cgagctcgcc atggccacgg ccctgcgcca gagcgaacgc cagtaccgcc tcctggccga
      301 aaacacggcc gacgtggtct gggtgctcag caccgagacc nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnng acactggacg gcgtcatcgc gcctgaatcc tggccggaga tgacaactgg
      481 gctgctcggc cggctggagg cttttcggga tggcgatccg gcggccaggc agcggatcga
      541 tcagatcctg ctgctgcgcc gggacggcac cactgtctgg accgaggtca gcacgacctt
      601 gatgttgaac gagcaccaag actgcgatct ggtcggcgtc acccgcgata tttcggtgcg
      661 caagcaggct gaagacgccc tggcgcagca agcctacatc ctggatcggc tggacgaggg
      721 tgtcagtgtg gccgaccagg ccggcaatct gatcttcacc aaccgctccc tggatgccat
      781 gttcggatac gctcacagcg aactggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ctggaccggc gaactgcgct
      901 gccggcgcaa ggacggcacg gtgttcacgg ccgccgcccg cgtcaaccag gtggagatgg
      961 ccggtcggcg cctggtggtc acggtccaat ccgatatcac cgagcagaag caggcccagg
     1021 cctggatgct caacgcgcag aagctggccg acctggggac gctggcggcc ggcgtggccc
     1081 atgaaatgaa ttcgcccttg caggtgatca ccttgccggc ggtggcgcca ttcgcctgca
     1141 cgtcgaatgc atcgaggcgc agatctctga tacaggctcg gcctggtcgg cgcgcgcacg
     1201 gccggggcat cccgacgagc cgaccgaggc atcgtgaagt gccgtcagtg cgcagcaaca
     1261 gcggaacagg gtcaatcaca tggcgaaacg gctcagcacc gcagaccccg gctttgccgc
     1321 cgaattccgg cgcttcctcg gcgagaagcg tgaggcggca cccgatgtcg atgctgccgt
     1381 cagcgtcatc gtcgccgacg tccgatcgcg cggcgatgcc gcgctgatcg agttcagccg
     1441 caagttcgat cgcctcgagc tcacgcgcga gacgctcagg atcggcgcgg acgagatcgc
     1501 cgcggcatcg aaggcagtcg acccggagat gcgcacggca ttgaccttcg cccgcgaccg
     1561 gatcaagtcc caccatgccc gccaactgcc gaaggacgat caatatcgcg acgacctcgg
     1621 cgtcgagctc ggctcgcgct ggacgccgat cgattcggtc gggctctatg tccctggtgg
     1681 caccgcgtcg tatccgagtt cggtcctcat gaatgcggtt ccggcgaagg tcgcgggcgt
     1741 cgcgcgcatc gccatggtgg tgccgacacc cgaaggacga ctcaatccgc tggtgctcgc
     1801 ggcggccgag cttgccggcg tcgaagagat ctaccgggtc gggggcgcgc aggcgatcgc
     1861 agctctcgcc tacggtaccg agacgatcgc gcccgtcgcc aagatcgtcg gccccggcaa
     1921 cgcctacgtt gcggcggcga agcggcaggt gttcggtgcc gtcggcatcg acatggtggc
     1981 gggaccgtcg gaagtactga tcatcgccga cagcgacaac gatccggagt ggatcgccgc
     2041 ggatctcctg gcgcaggccg agcacgacac ggccgcgcaa tcgatcctca tgacggacga
     2101 cgcggccttc gccgatcgcg tcgaggccgc ggtcgagcac cagctcgcag ctttgcctcg
     2161 aggcgagacg gcacgggcga gctgggcgac gttcggggca atcatactcc tcggatcgct
     2221 cgacgaggcg ccgccgctcg ccgaccggat cgccgccgag cacctcgaga tcgcgacgcg
     2281 agctcccgac gcgctggcag cgcgcatccg tcatgccggt gcgatctttc ttgggcattg
     2341 cacgcccgaa gtgatcggcg actatgtcgc cggctccaat catgtcctgc cgacggcacg
     2401 ctcggcccgt ttctcttcgg ggctcggcgt gctcgactac atgaagcgca cctcgatgtt
     2461 gcgcttgacg ccgcagtcat tcgcagcgct tgccccgcac gcgatgcgcc tcgccgaggc
     2521 cgaagggctc ggcggccatc gccgctccgt cgagatccgc ttggcacccg acgcgagcag
     2581 ccggaagagc acatgagcac cagcatgcgt gacgagcccg accccccgca tcccgatccg
     2641 aaatcgcgcc tcgttgcgat cacgctcgac gagcaatcga tcctgcgcag caatccgaac
     2701 atcgagcacg aacgccaagt cgcgatcttc gatatcctcg atgccaacca ctttgcagtt
     2761 gaggggcgca gcgacggccc cttcaagctc gatctgtcgg tcgtcgagga gcgtctcgcg
     2821 ctcgccatca cgaccgaggc cgatgcgccg gtcgcgacgc acctcctctc gctgtcgcca
     2881 ctgcgccgca tcatcaagga ctacttcgtc gtctgcgata cctattacga ggcgatccgg
     2941 tccgcgccgc catcgaagat ccaagcgatc gacatggggc ggcgcggcct ccacgacgag
     3001 gggagccgcg tgctcgccga gcgcctcaag gggaaagtcg agctcgatca cgacacggcg
     3061 cggcgcctct tcacgctgat ctgcgcgctg cgctcgaagg gatgagctct tgagccaggg
     3121 ggtgcccgga tcggtcctct tcgcgtgcac catgaatgcg gtgcgctcgg tgatggctgc
     3181 cgccatcctg cggcacctcg ccggcaaacg cgtctatgtg gaatcggcgg gcgtgcgggc
     3241 cggcgaaagc gatcccttcg tcgctgccgt gatgagcgag attggcatcg acgtctcaca
     3301 gcatcacccg cggacgatcg gcgaactctt cgacacgaac ttcgatgtcg tgatctcgct
     3361 gtcgcccgag gcgcatcacc acgcgatcga gatgacccgc accatggccg tcggcgtcga
     3421 gtactggccg acgctggatg cgaccgccaa ccgtggcggc agccgtgagc agatccttga
     3481 aggctaccgc ggcgtgcgcg acgccctctt ccggcgcatc aaggcccgct tcggtttcga
     3541 gggcggaccg tcggtgtagg cgggctcgag gcaatggagg cattgccacg cgagcggagc
     3601 gcctgttttt gcttgcactg caaaatgggt cagctatgct gaccagagtt gcaaccgacc
     3661 gaccgacgct ggcgtgcctc gaatggcgaa ttgccgcccg acggcagcgc ggtctagatt
     3721 gcagccttgg cagctctgtc gcgaaagcag gctgcggtcg ccctcgtggg cacggcgtcc
     3781 gatccggttg acgctgtggc gagtgcgggt ccgcggctcc agtttcgcgg tgtgatctcc
     3841 atgcagcgca ggcgcgttcg agtcgtcgac atctcgtcgg cggcgacgct ccggcacggc
     3901 atggagcttt cgtgcgcctg ccatcgggga ctgaggcctt agagacgaca gctcgtgagg
     3961 gcaatgacat gatcgacaag cctgagctcg acaccgaatt agatgatctc caggccgccg
     4021 acggcctcga catcgacgtg ggcgccgccg acaccatgcc ggccgtcgcg ggcttgttcg
     4081 atcgaaaaaa ggagcacgat gcctgcggcg tcggcttcgt cgnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnctgct cgagaacctc gagcaccgcg
     4201 gtgccgtcgg cgccgacccg ctcgcgggcg atggcgcggg catgctgatc cagcttccgg
     4261 acgcattcct gagagccgag atggcagcgg cgaagatcaa gctgccgaag cccggctggt
     4321 atgcggtcgg ccagctcttc atgccgcagg acgagcggct gcgcgctcac tgcgagcgcg
     4381 tctgggcccg catcctgcgg gaggaagggc tcgtcctgct cggctggcgc tcggtgcccg
     4441 tcgacaactc gaagctctcc gagatggtga aggagaccga gccggtccac cgccagatct
     4501 tcgtcggccg ccccgagggc atggccgacg aggccgagtt cgagcgcaag ctgtacctcg
     4561 tccgaaaggt cgtctcgaac gcgctcttct acgcctacaa ggagcgcgac atcggccact
     4621 acaccgtgtc gctgtcgagc cggacgctgg tctacaaggg gatgttcctg tccttccagt
     4681 tgaaggccta cttcaaggac ctctccgacc cgcgcatggc gtcgacgctg gcgctcgtgc
     4741 atcagcgctt ctcgaccaac acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nagatcaaca ccctgcgggg caacgtcaac tggatggctg cgcggcaggc ctccgtctcc
     4861 tccccgctct gggggaagga catctccaag ctctggccga tctcctacga gggccagtcg
     4921 gatacggcct gcttcgacaa cgctctcgag ttcctggtga tgggcggcta cagcctcgct
     4981 catgcggcga tgatgctgat cccggaggcc tgggccggca acccgctgat ggatgggaag
     5041 cgtcgcgcct tctacgagta ccatgcctgc ctcatggagc cctgggacgg cccggccgcc
     5101 atggccttca cagatggcct ccagatcggc gcgacgctcg atcgcaacgg tctccgcccc
     5161 gcccgctatc tcgttacgca cgacggcctc gtcgtcatgt cgtcggagag cggcgtgctg
     5221 ccgatcgagg aatcacgcat cgcgaagaag tggcgcctcc agcccggcaa gatgctgctc
     5281 atcgacatgc agaagggtcg catcatctcc gatgacgagc tcaaggccga cctcgcctcg
     5341 cgatacccgt atgagcagtg gctgaagagc acgcagatcc aggtcggcga cctgccgctg
     5401 ccgaagaaga tcgccaagaa gaagtcgaac gtcgcgctcc tcgatctgca gcaggccttc
     5461 ggctacaccg aggagagcct gaagttcctg atgagcccga tggcgcatac cgggcaggaa
     5521 gccgtcggct ccatgggcac tgatacgccg gtctcggtgc tctcctcgaa gccgaagctc
     5581 ctccacacct acttcaagca gaacttcgcg caggtgacga acccgccgat cgattcgatc
     5641 cgcgaggagc tcgtcatgag cctcgtctcg ttc
//
LOCUS       DAORNH010000300         5672 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10541_length_5672_cov_2.732954, whole genome shotgun sequence.
ACCESSION   DAORNH010000300 DAORNH010000000
VERSION     DAORNH010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10541_length_5672_cov_2.732954"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..162
                     /locus_tag="PK264_21600"
                     /pseudo
     CDS             1..162
                     /locus_tag="PK264_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970243.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(889..2709)
                     /gene="glmS"
                     /locus_tag="PK264_21605"
     CDS             complement(889..2709)
                     /gene="glmS"
                     /locus_tag="PK264_21605"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRD78500.1"
                     /translation="MCGIVGILGQKPVAIDLVDALRRLEYRGYDSAGVATVENGHLER
                     RRAEGKLRNLADKLTSEPLKGTAGIGHTRWATHGGPSERNAHPHMTDKVSVVHNGIIE
                     NFRELKDELIRAGLTFESETDTEVVAQRTTREMRQGAAPVEATRRALGRIRGAFALGI
                     IFSGHDNLMIGAREGSPLAIGHGDGEMYLGSDAIALAPFTSQITYLEEGDWAILTRQS
                     VEIRDRHNRRVERPMVTSSASGFLVDKGNHRHFMLKEIHEQPEVISHTLTNYIDMGTS
                     RVKFPDLGIDLAKISRVTISACGTAYYAGLVGKYWLERYARIPVEIDVASELRYREAP
                     LPKDGLAVFVSQSGETADTLATLRYCKANGQRIASVVNVRTSTIARESHAILPTLAGP
                     EIGVASTKAFTCQLSTLACLALAIGRARNAISADQERDLVKALTEVPRHISSILRDEK
                     RYEELAHSLSKARDVLYLGRGVSYPIALEGALKLKEISYIHAEGYAAGEMKHGPIALI
                     DENVPVVIVAPEDDLFEKTVSNMQESAARGGQIVLVSNADPDKTGVETAAHLRMPPVH
                     PFASPLVYAVPMQLIAYHTAVLMGTDVDQPRNLAKSVTVE"
     gene            complement(2796..4169)
                     /gene="glmU"
                     /locus_tag="PK264_21610"
     CDS             complement(2796..4169)
                     /gene="glmU"
                     /locus_tag="PK264_21610"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRD78501.1"
                     /translation="MSKRTLLTVILAAGKGTRMKSALPKVLHRIAGRSMLGHVMATAK
                     QAGADRLAVVIGPGMEDVRAEVLRHAPGSDVYIQDPQLGTAHAVLAARKALAGHAGDV
                     LILYADTPLIRADTLQRLVEGLDQRGGVGVLGFEAADPTGYGRLVTDTNGALLAIREH
                     KDASDAERVIRLCNSGVMVFRCPSLVGLLDRVGNANAKGEYYLTDTVEIARRDGLGAR
                     AIVCPEDEVLGVNSRDQLAAAEAIWQARARHAAMLGGVTMVAPETVWLSYDTTLGQDV
                     VIEPNVVFGPGVTVADRVDIRANCHIEGAHIASGARIGPFARLRPGADLGADVHIGNF
                     VEVKNVTMAAGAKANHLAYLGDGSVGEDANIGAGTIFCNYDGFNKHTTTVGKGAFVGS
                     NSALVAPVTIGDGAFVGSGSVITRDVAPDALAVERSVQEERPEWARKFRTLMQRKKQA
                     KRDKGAS"
     gene            4491..>5345
                     /locus_tag="PK264_21615"
     CDS             4491..>5345
                     /locus_tag="PK264_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRD78502.1"
                     /translation="MANFPTHIAIGGLVAGTLATVTLAADVVAPENLVVVTVAGILGS
                     VLPDIDLKDSRPSRAMFNGLAAFFSAAVLFTIAYKYSIVEMWIMLLGTFLLVRYGAHS
                     MFHRMSYHRGIYHSLLAAVFFAFATAAAYKHVLGRHEGVAWLAAAFMFVGYLTHLILD
                     EIYSVDVMDTRIKASFGTAIKPIDFKHPGHTAVMALAAAAMFMVTPSTRTFVDGMSSA
                     PMWSALQQRLLPRDKWFGVIGPIRAMAGPATPREDTGGITTGSLPDTLMVAGDTHQPA
                     RPKVQLRLP"
     assembly_gap    5346..5355
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5357..>5672)
                     /locus_tag="PK264_21620"
     CDS             complement(5357..>5672)
                     /locus_tag="PK264_21620"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRD78503.1"
                     /translation="PRIAAPSPPPPAPGPPPAVAVPPSSTPPPAATPVPSRHARARPV
                     CRSGRREGDWHIVGEGESLWSIAERYYDDGNRYPRILRANRDRIDDPDVILPCQRLRV
                     PR"
BASE COUNT          878 a         1864 c         1931 g          989 t
ORIGIN      
        1 tccggcatcg gcggcgtggc gcgtcccggc atcgtgcatc gcctcgacaa agataccagc
       61 ggcgtgatgg tcgccgcgaa aaacgacgcc acgcacgcga gtttggcggc gcaattcgcc
      121 aagcatacga tcgaacgtgt ctatctcgcc ctggtgcgcg gccgctggag ccgcggcgac
      181 ggctgccggt gccgctgcag gcgcatcggc cgtcacggcc gatgggccgc cggcaaggcg
      241 cttctcgtcg gccagagcct ggtcgttgag gcccgcctcg cgataggcgg ccgcgcgatt
      301 gcgcagcgcc tcgacacgct cggcgtcgct gaggccaccc ttcaggtaga gcgcgcccgt
      361 gaggtcggca atcgcctggg ccggcttgct ctgcttccgg tagatctggc cccgcaggtg
      421 cagcgacttg gcgatgaccg agggcggcgc gccgctgacg ccaagtgccc ggttcgcctg
      481 ggtgagcgcg atatcgagct tgccggcctg gaaggccttc tgcgctacct cgtaggcgcg
      541 cgtcgcctcg gccgggtccg ctttcgcaac cgatcccgct tcgtcggccg ccttcgacgc
      601 tttggtcggg gcctttctct tcgtgggctt ggctgcaggc gcgggcgccg cagctggtgc
      661 agcagccgga gccggtgcag gagcctgggc cttcacgtca ctgacgaggc cagcgcccat
      721 cgctcccgac cacacgatgg cggcgagcgc ggctggcgcg agggcgcgag caagaatagc
      781 catggtcaac ctcgagaatc actcgcacag aacctgcacc cgcaggaggc taagtcaagc
      841 atggcaatga ggcaattctt cgaggcgaac ccgcagagtt aatcacaact actcgacagt
      901 gaccgacttg gcgaggttgc ggggctggtc gacgtcggtg cccatgagca cggccgtatg
      961 ataggcgatg agctgcatcg ggaccgcgta gacgagcggc gaggcgaagg gatgcaccgg
     1021 tggcatcctg aggtgcgccg ccgtctcgac gcctgtcttg tcggggtcgg cgttggagac
     1081 gagcacgatc tggccgccgc gagcggcgct ctcctgcatg ttggagacgg tcttctcgaa
     1141 gaggtcgtcc tccggcgcga cgatcaccac cggcacgttc tcgtcgatca aggcgatcgg
     1201 cccgtgcttc atctcgccgg cggcatagcc ctcggcgtgg atgtagctga tctccttgag
     1261 cttcaaggcg ccttcgaggg ctatcggata gctgacgccg cggccgagat agagcacatc
     1321 gcgcgccttc gagagggagt gcgcgagttc ctcgtagcgc ttctcgtcgc gcaggatcga
     1381 cgagatgtgg cgcggcacct cggtcagcgc cttgacgaga tcgcgttcct ggtcggccga
     1441 gatggcattg cgtgctcgac cgattgcaag cgcgaggcag gcgagcgtcg agagctggca
     1501 ggtgaaggcc ttcgtcgaag cgacgccgat ctcggggcca gcgagcgtcg gcaggatcgc
     1561 gtggctctcg cgggcgatgg tggacgtgcg cacgttgacg accgaggcga tgcgctggcc
     1621 gttggccttg cagtagcgca gcgtcgcgag cgtatccgcc gtctcgcccg actgcgacac
     1681 gaacacggca aggccgtcct tcggcagcgg cgcctcgcgg tagcgcagct cgctcgccac
     1741 gtcgatctcg acggggatgc gggcatagcg ctcgagccag tacttgccga cgaggcccgc
     1801 atagtaggcc gtaccacagg ccgagatggt gacgcgcgaa attttggcga gatcaatgcc
     1861 gaggtcgggg aacttcaccc ggctcgtgcc catgtcgatg tagtttgtca gcgtgtggct
     1921 gatcacctcc ggttgctcat ggatttcctt gagcatgaag tggcggtggt tgcccttgtc
     1981 gacgaggaaa cccgaagccg acgaggtgac catcggccgc tcgacgcggc ggttgtggcg
     2041 gtcgcggatc tcgacgctct ggcgcgtaag gatggcccag tcgccctcct ccagataggt
     2101 gatctggctc gtgaacgggg cgagcgcgat ggcgtccgag cccaaataca tctcgccgtc
     2161 gccgtggccg atggcgagcg ggctcccttc gcgggcgccg atcatcaggt tgtcgtggcc
     2221 ggagaagatg atgccgagcg cgaaagcgcc gcggatgcga ccgagcgcgc gcctggtcgc
     2281 ctcgacgggc gcggcgcctt ggcgcatctc gcgcgtggtg cgctgcgcga cgacctcggt
     2341 gtcggtctcg ctctcgaagg tcaagccggc gcggatcagc tcgtccttga gctcacggaa
     2401 gttctcgatg atgccattgt ggacgaccga gaccttgtcg gtcatgtgcg ggtgagcgtt
     2461 gcgctcgctt ggaccgccgt gtgtcgccca gcgcgtatgg ccgatgccgg ccgtgccttt
     2521 cagcggctcc gaggtcagct tgtcggcgag gttgcgcagc ttgccctcag cgcggcggcg
     2581 ttcaagatgg ccgttctcga ccgttgcgac gcccgctgaa tcatagccgc gatactcgag
     2641 gcggcggagc gcatcgacga ggtcgatcgc caccggcttc tggccgagaa ttccgacgat
     2701 gccgcacatg tttccatctc ctggagctgc ctcgccggcc tagtgcttcg tgacgcggcg
     2761 tacaattcaa accgtgtttc aatgtgttgc tgaggtcagc tcgcgccctt gtcgcgcttc
     2821 gcctgcttct tgcgctgcat gagcgtgcgg aatttgcgcg cccactcggg ccgctcctcc
     2881 tgcacgctgc gctcgaccgc gagtgcatcg ggtgccacgt cgcgcgtgat cacgctgccc
     2941 gatccgacga aggcaccgtc gccaatggtg acgggggcga cgagcgccga attcgatccg
     3001 acgaaggccc ctttgccaac ggtcgtcgtg tgcttgttga agccgtcgta gttgcagaag
     3061 atcgtgcccg cgccgatgtt cgcgtcctcg ccgacgctgc cgtcgccgag ataggcgagg
     3121 tggttcgcct tggcaccagc ggccatcgtc acgttcttca cctcgacgaa gttgccgatg
     3181 tgaacgtcgg cgccgagatc agcgccgggc cgcaggcgcg caaacggtcc gatcctcgcg
     3241 ccggacgcga tatgcgcgcc ctcgatatga cagttcgcgc ggatgtcgac gcggtccgcg
     3301 accgtgacgc ccggcccgaa caccacgttc ggctcgatca ccacgtcttg accaagcgtg
     3361 gtgtcgtagg agagccagac cgtctccggc gccaccatcg tgacgccgcc gagcatggcg
     3421 gcatggcgcg cgcgggcctg ccagatcgcc tcggccgcgg cgagctggtc acgcgagttg
     3481 acaccgagca cctcgtcttc cggacagacg atggcgcggg caccgaggcc atcccgccgc
     3541 gcgatctcga cggtgtcagt cagatagtac tctcccttcg cattggcgtt gccgacgcgg
     3601 tcgagcaggc cgacgaggct cgggcaccgg aagaccatca cgcccgagtt gcagaggcgg
     3661 atcacccgct cggcgtcgct cgcgtccttg tgctcgcgga tggcaaggag cgcgccgttc
     3721 gtgtccgtga cgaggcgccc gtagccggtc ggatcggctg cttcaaaacc gagcacgccg
     3781 acaccgccgc gttgatcgag accctcaacg aggcgctgca gcgtgtcggc gcggatgagc
     3841 ggagtgtcgg catagagaat gaggacatca ccggcatgcc ccgcgagggc cttgcgcgcg
     3901 gcgagcacgg cgtgcgcggt gccgagctgc gggtcctgga tatagacgtc cgagcccggc
     3961 gcatgccgca gcacttcggc gcgcacgtcc tccatgcccg gcccgatgac cacggcgagg
     4021 cggtcagcgc cggcctgctt cgcggtcgcc atgacgtggc cgagcatcga gcggcctgcg
     4081 atcctgtgca ggaccttcgg cagcgcggac ttcatgcgcg tgcccttgcc cgcggccagg
     4141 atgacggtca gcagtgtccg cttgctcatg gcgctccgtg cagagtcagg tgtttgcgac
     4201 agcgtggcct cgaccggatg ccacggccaa tccgtggctg tcgattgcaa agaacatgcc
     4261 cttccccagc ggcatcgggc gacatcgcaa atggttaatt ccgagcgcct tatgtgcggg
     4321 atcgcgggat gatcgtcaat atcgtcgggc ggcgatgcac cgatctgcat tcggtgccac
     4381 ggcgtgccga cgtccacagt cattgtcggc caaccggcac tcagctctcg cgacggcggc
     4441 gagaatcaga tgaaccagaa tctccgagcc acgccagcgg agaccgcctc atggccaact
     4501 tcccgaccca catcgccatc ggcggcctcg tcgccggcac gcttgccact gtgacgctcg
     4561 ccgccgacgt ggtcgcaccg gagaatctcg tcgtggtcac tgtcgccgga attcttggca
     4621 gcgtcctccc ggacatcgat ctgaaggatt cgcgcccgag ccgggccatg ttcaacgggc
     4681 ttgccgcctt cttctcggca gctgttctgt tcacgatcgc ctacaagtac tcgatcgtcg
     4741 agatgtggat catgctgctc ggaacgttcc tgcttgtgcg ctacggcgcg cactcgatgt
     4801 tccaccgcat gtcgtaccac cgcggcatct atcattcgct gctggcggcc gtcttcttcg
     4861 ccttcgcgac cgccgccgcc tacaagcatg tccttggccg gcacgagggc gtcgcctggc
     4921 tcgctgcggc cttcatgttc gtcggctacc tgacgcatct gatcctcgac gagatctaca
     4981 gcgtcgacgt catggatacg cgcatcaagg cgtcgttcgg caccgccatc aagccgatcg
     5041 acttcaagca tccgggccac acggccgtca tggcactcgc agctgcggcg atgttcatgg
     5101 taacgccgtc gacgcgcacc tttgtcgacg gcatgtcgtc ggcgccgatg tggtcggcgc
     5161 tgcagcagcg cctgctgccg cgcgacaagt ggttcggggt gatcgggccg atccgcgcca
     5221 tggccgggcc ggcgacgccg cgcgaggaca cgggcggcat cacgaccggc tcgctgccgg
     5281 acacattgat ggtcgccggc gacacgcacc agccggccag gccgaaggtg cagctcaggc
     5341 tgccgnnnnn nnnnnatcat cgcggcacgc gcagccgctg gcatggcaag atgacatcgg
     5401 gatcgtcgat ccggtcccga ttggcccgca ggatgcgcgg gtagcgattg ccatcatcat
     5461 agtagcgctc ggcgatcgac cagagcgatt cgccctcgcc gacgatgtgc caatcgcctt
     5521 cgcggcgacc cgatcggcag accggcctgg cgcgcgcatg gcgggatggc accggagttg
     5581 ctgccggcgg tggtgtcgac gacggcggaa ccgcaacggc gggcggaggt ccaggtgccg
     5641 gcggcggcgg cgacggagcg gcgatgcggg gc
//
LOCUS       DAORNH010000301         5653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10589_length_5653_cov_2.832797, whole genome shotgun sequence.
ACCESSION   DAORNH010000301 DAORNH010000000
VERSION     DAORNH010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10589_length_5653_cov_2.832797"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="PK264_21625"
     CDS             complement(<1..1442)
                     /locus_tag="PK264_21625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRD78504.1"
                     /translation="MTQREARRKGGKSTESELVLVALGGLGEIGMNVYLYGLGPEDDR
                     QWLMVDLGMTFPDFSVDPGVDVILPDLRFIEEQVKSLVGIVLTHAHEDHIGAVIDLWP
                     RLKAPIYATPFTAAMLRAKLTEFSSLMRLPIREVALGSRFNVGPFDLELVTVTHSIPE
                     PNGLAIRTPHGLVLHTGDWKLDADPVIGAPADEAKLASLGEEGVLAIVCDSTNAFREG
                     RSPSEGAVTRSLVEIIKGARHRVAVTTFASNVARIRAVADAARASGRKLVIAGRALHR
                     VIEVAMETGYLPKGFAYYDQNEFKHIPPDKALALVTGSQGEHRAAIARIAQGEHPAIS
                     FGAGDLVIFSSRTIPGNEKVVGRVQNALARLGCEIVTDAEKLVHVTGHPRREELRQMY
                     AWTKPRIAVPMHGEMRHLKEHAKLAREAGVPDVFTPINGEVLRLAPAPRLIDEAPVGR
                     IYRDGRMLIPAGDGPVKERRKLAEVGVVVVA"
     gene            complement(1544..>1856)
                     /locus_tag="PK264_21630"
     CDS             complement(1544..>1856)
                     /locus_tag="PK264_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78505.1"
                     /translation="RRTACLAGLGSRATASDVLASLADALDGRLRQWDGDLGISSCLA
                     DWTARSYPIGTAMSVNTGHGLVRGNYAGLGPDGTLLLDQGAGRVTLTFGDVTIDSRPP
                     A"
     assembly_gap    1857..1884
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1885..2307)
                     /locus_tag="PK264_21635"
     CDS             complement(<1885..2307)
                     /locus_tag="PK264_21635"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRD78506.1"
                     /translation="MPVSGDGARHRIVRLAEVGSTNSEALRRAAMGEAGPLWIAAERQ
                     IAGRGRSGRAWDGLPGNLFATLLMSPECAIARIGELALVAGLAAHDAASAALAHETPA
                     SAAGLKLKWPNDLECDGAKLAGILCESVVVAAGARVAIG"
     gene            complement(2322..3770)
                     /gene="nuoN"
                     /locus_tag="PK264_21640"
     CDS             complement(2322..3770)
                     /gene="nuoN"
                     /locus_tag="PK264_21640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HRD78507.1"
                     /translation="MQALAAYQAIAPEIVLVGAALLLLVYGVFRPETADEAEPIGWAA
                     VAAIAFAGYWVVRQTGSTALFDKSLVFDGFTQFMKLLSLVGAAGALILSFDYLREARI
                     LKFEFPVLILLATAGMMLMISANDLMALYLGLELQSLALYVIAAFHRDQVRSSEAGLK
                     YFVLGALSSGMLLYGASLIYGFTGTTSFTGIAAAIKAQGGTASIGLVFGLVFLMVGLA
                     FKVSAVPFHMWTPDVYEGSPTPVTAFFAAAPKVAAMALLIRTLDGALPGIRAEWTQIV
                     VFLAIASMLLGAFAAIGQTNIKRLMAYSSISHMGYALVGVAAGNAEGIQGTLVYLAIY
                     LAMTLGAFACILSMRRSDGPVEDISSLAGLSQTNRPLAYILAALLFSLAGIPPLAGFF
                     AKMYVFLAAMKAGGNLYYLAIIGVLASVVGAYYYLRIIKIMFVDEPKEGFVAVAPKAG
                     FIMGVATAFVILYAVWPAPLVEAAGRAARSLF"
     gene            complement(3787..>3986)
                     /locus_tag="PK264_21645"
     CDS             complement(3787..>3986)
                     /locus_tag="PK264_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRD78508.1"
                     /translation="GYLPENVPLYADMTPREIAVLAPLVFLTILFGVWPAPILDVTAR
                     SVQRLVTNYEAALKAAAALAN"
     assembly_gap    3987..3996
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3997..5341)
                     /locus_tag="PK264_21650"
     CDS             complement(<3997..5341)
                     /locus_tag="PK264_21650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRD78509.1"
                     /translation="MVTSAPPLLSLVTFLPLVGVVAIAFLNPEARRNARWIALWTTVA
                     TFLVSLLVWHGFDKSNPGFQFVEEYRWLSDTIKYKMGVDGISMLFVILTTFLMPLCIL
                     ASWEAIEDRVKEYMIAFLVLETLMIGVFCALDLVLFYLFFEGGLIPMFLIIGVWGGKR
                     RVYASFKFFLYTLIGSVLMLLAMLYMYWHAGTTDIVKLLATRFPRDAQWWLWLAFFAS
                     FAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFLRFSLPMFPHASQELAW
                     LVFALSCVAIVYTSLVALAQEDMKKLIAYSSVAHMGYVTMGIFTFTVQGIDGAIFQML
                     SHGLVSGALFLCVGVVYDRMHTREIAAYGGLVNRMPIYALFFMVFTMANVGLPGTSGF
                     IGEFLTLLGAFKVSTWVAAIATTGVILSAAYALYLYRRMIFGVLDKPALKAIADMS"
     gene            complement(5341..>5653)
                     /locus_tag="PK264_21655"
     CDS             complement(5341..>5653)
                     /locus_tag="PK264_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRD78510.1"
                     /translation="WLPAATARAFRPLYLFFLNKWYFDELYDRLFVRPAHAIGRVLWR
                     TGDGKIIDGLGPDGIAARVLDAARGAVRLQTGYVYHYAFAMLIGIAALITWFMMTGKS
                     V"
BASE COUNT         1069 a         1748 c         1984 g          814 t
ORIGIN      
        1 gccacgacga ccacgccgac ctcggcaagc ttgcggcgct ctttcactgg gccgtcgccc
       61 gccggaatga gcatgcggcc gtcgcgatag atgcggccga ccggcgcctc gtcgatgagg
      121 cggggcgccg gcgcaaggcg gaggacctct ccgttgatcg gcgtgaacac gtccggcacg
      181 ccggcctcgc gggcgagctt ggcgtgctcc ttcagatggc gcatctcgcc gtgcatcggg
      241 acggcgatcc gcggcttcgt ccatgcatac atctggcgaa gctcctcgcg ccgcgggtgc
      301 ccggtgacgt ggacgagttt ctcggcgtcc gtgacgatct cgcagccgag gcgggcgagc
      361 gcgttctgca cgcggccgac gaccttctcg ttccccggga tcgtgcgcga ggaaaagatc
      421 acgagatcgc ccgcgccaaa cgagattgcc gggtgctcgc cctgcgcaat gcgggcgatg
      481 gctgcgcggt gctcgccctg actgccggtt accaatgcca gcgccttgtc cggcgggatg
      541 tgcttgaact cgttctggtc gtagtaggcg aagcccttgg gcaggtagcc cgtctccatc
      601 gcgacctcga tcacgcgatg cagcgcgcgg ccggcgatca caagcttgcg gcccgaagcg
      661 cgggcggcat cggcgacagc gcggatgcgc gcgacatttg aggcgaaggt ggtgacggcg
      721 acgcgatgcc gtgctccctt gatgatctcg acgagcgagc gcgtgacggc tccctccgac
      781 ggcgaacggc cttcgcggaa ggcgttggtc gaatcgcaga cgatggcgag aacaccctcc
      841 tcaccgaggc tcgcaagctt ggcctcgtcc gccggtgcgc cgatcacggg atcagcgtcg
      901 agcttccagt cgccggtgtg caggacgagg ccatgcggcg ttcggatggc gaggccgttc
      961 ggctccggga tcgagtgcgt aaccgtgacg agctcgagat cgaaggggcc gacattgaaa
     1021 cggctgccga gcgccacctc gcggatcggc aggcgcatca ggctcgagaa ttcggtgagc
     1081 ttggcgcgga gcatcgccgc cgtgaacggg gtcgcataga tcggcgcctt tagccgcggc
     1141 cacagatcga tgacggcgcc gatgtggtcc tcgtgcgcgt gggtcagcac gatgccgacg
     1201 aggctcttca cctgctcctc gatgaagcga aggtccggca ggatgacatc gacgccggga
     1261 tcgaccgaaa agtcgggaaa cgtcatgccc agatcgacca tcagccattg gcgatcgtcc
     1321 tctggtccga ggccgtagag gtagacgttc atgccgattt cgccgaggcc gccgagcgcc
     1381 acgagcacga gctcgctttc ggtcgatttg ccccctttgc ggcgcgcttc gcgctgtgtc
     1441 atgccgttcc aatccagtcc tctgcaaggc gagcgtgcga aagcctgcct gacctatata
     1501 tgacagccgt cctacaccag gattgccctg tcgtggtcca atttcacgcg ggcggacggg
     1561 aatcgatcgt cacatcgccg aacgtgagcg tcacgcgacc ggcgccctga tcgagcagca
     1621 gcgtgccatc ggggccaagc cccgcgtaat tgccgcgcac gaggccgtgc ccggtgttga
     1681 cggacatcgc cgtgccgatc gggtatgagc gggcggtcca atcggcgaga catgatgaga
     1741 tgccgaggtc gccgtcccac tgcctgaggc gaccatccag ggcatcggcg aggctcgcga
     1801 gaacgtcgct cgcggtcgcg cgcgagccga gcccggcgag gcacgccgtc cgccgcnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnngccgat cgccacacgc gctcctgccg cgacgacgac
     1921 gctctcacag aggatgccgg cgagcttggc tccatcgcat tcgaggtcat tgggccactt
     1981 gagcttaagg cccgccgcgc tcgcgggcgt ctcatgagcc agcgccgcag aggcggcatc
     2041 gtgggcggcg aggcccgcga cgagggcgag ctcgccgatg cgggcgatgg cgcactcggg
     2101 tgacatcaag agggtcgcga agagattgcc cgggagcccg tcccaggcgc ggcctgatct
     2161 gccgcgaccg gcgatctggc gctcggccgc gatccagagc ggccccgcct cccccatcgc
     2221 tgcgcgacgc agcgcttccg aattggtcga gcctacctcg gcaagccgga cgatgcggtg
     2281 gcgcgcgcca tcgccgctga ccggcaaact cgagcgcgcc gtcagaagag gctccgtgca
     2341 gcgcggccgg cggcttcgac gagcggggcc ggccagaccg catagaggat gacgaaggcc
     2401 gtcgcgacac ccatgatgaa cccggccttg ggcgcgacgg ccacgaagcc ttccttcggc
     2461 tcgtcgacga acatgatctt tatgatgcgc aggtagtagt aggcgccgac cacgctcgcg
     2521 agcacgccga tgatggcgag atagtagagg ttgccgccgg ccttcatggc ggcgaggaac
     2581 acgtacatct tggcgaagaa cccggcgagc gggggaatgc cggcgagcga gaagagaagg
     2641 gctgccagga tgtaggcgag cggccggttg gtctgcgaga ggccggcgag cgagctgatg
     2701 tcctcgacgg gcccgtccga gcggcgcatg gagaggatgc aggcgaaggc accgagcgtc
     2761 atggcgaggt agatggcgag atagacgagc gtgccctgga tgccctccgc attgccggcc
     2821 gcgacgccga cgagcgcata gcccatgtgg ctgatcgagg agtaggccat caggcgcttg
     2881 atgttggtct gcccgatcgc cgcgaaggcg ccgagcagca tcgaggcaat ggcgaggaag
     2941 acgacgatct gcgtccactc ggcgcgaatg ccgggcagcg cgccgtcgag cgtgcggatc
     3001 aggagcgcca tggccgcgac cttgggcgcc gcggcgaaga aggctgtgac cggcgtcggc
     3061 gagccctcat agacgtcggg cgtccacatg tggaaaggga cggccgacac cttgaaggca
     3121 aggccgacca tcaggaagac gaggccgaag acgaggccga tgctcgccgt gccgccctgc
     3181 gccttgatgg ccgcagcgat gcccgtgaac gaggtcgtac ccgtgaagcc gtagatcagc
     3241 gaggcgccgt agagcagcat gcccgaggag agtgcgccga gcacgaagta cttgaggccg
     3301 gcctcactcg agcgcacctg atcgcggtgg aaggcagcaa tgacgtagag ggcgaggctc
     3361 tgcaactcga gcccgaggta gagcgccatc aggtcgttgg cggagatcat cagcatcatg
     3421 cccgcggtcg cgagtaggat gagcaccggg aactcgaact tcaggatgcg cgcctcgcgc
     3481 aggtagtcga acgagaggat cagcgcgccg gcagcgccga cgagggagag gagcttcatg
     3541 aactgcgtga agccgtcgaa gacgaggctc ttgtcgaaga gcgcggtcga tccggtctgg
     3601 cgcacgaccc agtagccggc gaaggcgatc gccgcaaccg cggcccagcc gatcggctcg
     3661 gcctcgtccg cggtctcggg gcggaacacg ccgtagacga gcagcaggag cgccgcgccg
     3721 acgagcacga tctcaggggc gatggcttga tatgcggcga gcgcctgcat gagggtctcc
     3781 ggaacgtcag ttggcgagcg cggcggcggc tttgagtgct gcctcgtagt tggtaacgag
     3841 gcgctgcacc gagcgcgcgg tcacatcgag aatcggcgcc ggccacactc cgaagaggat
     3901 ggtgaggaag acgagcgggg cgagcaccgc gatctcgcgc ggggtcatgt cggcgtagag
     3961 cggcacgttc tcgggcaggt agccgannnn nnnnnnggct catgtcggcg atcgccttca
     4021 gggcaggctt gtcgagcacg ccgaagatca tccgccgata gagatagaga gcgtaggccg
     4081 ctgagaggat cacgccggtt gtggcgattg cggcaaccca ggtcgacact ttgaacgcgc
     4141 cgagcagggt gaggaactcg ccgatgaagc ccgaggtgcc gggcagcccg acgttcgcca
     4201 tggtgaagac catgaagaac agcgcataga tcggcatgcg gttgacgagc ccgccatagg
     4261 ccgcgatctc gcgcgtgtgc atgcggtcgt agacgacgcc gacgcacagg aagagggcgc
     4321 ccgagacgag cccgtgcgag agcatctgga agatggcgcc gtcgatcccc tgcaccgtga
     4381 acgtgaagat gcccatcgtc acgtagccca tgtgggcgac tgaggaatag gcaatgagct
     4441 tcttcatgtc ctcctgcgcg agcgccacga gcgaggtgta gacgatcgca acgcaggaga
     4501 gcgcgaagac gagccacgcg agctcctggc tcgcatgcgg gaacatgggg agcgagaagc
     4561 gcaggaaacc gtagccgccc atcttgagga ggatgcctgc aaggatcacc gagcccgcgg
     4621 tcggcgcctc gacgtgggca tcggggagcc aagtgtgcac cggccacatc ggcatcttca
     4681 ccgcgaacga agcgaagaaa gcgagccaga gccaccactg cgcatcacga gggaagcgcg
     4741 tcgcgagcag cttcacgatg tcggtcgtgc cggcgtgcca gtacatgtag agcatggcga
     4801 gcagcatcag cacgctgccg atcagcgtgt agaggaagaa cttgaagctc gcgtagacgc
     4861 gccgcttgcc gccccacacg ccgatgatca ggaacatcgg gatgaggccg ccctcgaaga
     4921 acaggtagaa gagcacgaga tcgagcgcgc agaagacgcc gatcatcagc gtctcgagga
     4981 cgaggaaggc gatcatgtac tccttgacgc gatcctcgat cgcctcccag ctcgcgagaa
     5041 tgcagagcgg catgagaaag gtcgtcagga tcacgaacag catcgagatg ccgtcgacgc
     5101 ccatcttgta cttgatggtg tcggagagcc agcggtactc ctcgacgaac tggaagccgg
     5161 ggttcgactt gtcgaagccg tgccagacga ggagcgagac gagaaacgtc gcaaccgtcg
     5221 tccagagcgc gatccagcgg gcgttgcgcc gcgcctcggg attgaggaag gcgatggcca
     5281 ccacgccgac gagcggcagg aacgtgacca gggacaggag cggcggtgcc gaagtgacca
     5341 tcagacgctc tttcccgtca tcatgaacca ggtgatcagc gccgcaatgc cgatcagcat
     5401 ggcgaacgcg tagtggtaca cgtagcccgt ctgcaggcgg accgcgccac gtgccgcgtc
     5461 aagcacgcgc gcggcgatgc catccggacc gaggccgtcg atgatctttc cgtcacccgt
     5521 ccgccacagt acgcggccga tggcgtgcgc cgggcggacg aagaggcgat catagagctc
     5581 gtcgaagtac cacttgttga ggaagaacag gtagagcggc ctgaaggcgc gcgccgtcgc
     5641 cgccggcagc cag
//
LOCUS       DAORNH010000302         5643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10607_length_5643_cov_2.597888, whole genome shotgun sequence.
ACCESSION   DAORNH010000302 DAORNH010000000
VERSION     DAORNH010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10607_length_5643_cov_2.597888"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1814)
                     /locus_tag="PK264_21660"
     CDS             complement(<1..1814)
                     /locus_tag="PK264_21660"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRD78511.1"
                     /translation="MAGLIPRVTWGEFMLASLETDPVLRIETLGRLDISFGGRQIKIR
                     SQKAKAMIAYVALADHGRESRRRLVGMFWGNSPQRQAQQSLRHCVGVLRRQFRLARCG
                     VLGIDPRELRLDQARVRTDIAEILESVEGGVVPERLLSPDAVDQSFLADLDGLDEEFD
                     TWLMSERSKISDRILRSLERQLYDPRRTSGQIEIARSLLRLDPTHDEATRALMRAYAA
                     QGDLTAALRVAKAQEDVLVREHGTGPTPETRRLTAEIRECAAAPVAGGPVVPITTPTV
                     VRIQPRTNKLVIMVAPLAMEAVSPERRYLCDGFRRELIASLILFREWHVRDGVKPTGE
                     ETTADEYALEAHASETRDGLRLVFTLKEGGSNEFVWSERLLLSLGEWTTAQQSVVRQL
                     ASSLNVSISEGRLARVVPQRDTAPLIFDQWLKGQQLIRSFRRDDWHEARRIFEEIIQA
                     APDFGPAYSSRAQLENIIHLAHHGLMRDAKGESQALDFARRAIGIDRGDSRAQLSLAW
                     SHLMLEQFDLAARHYEHALRLNDNDPWTTISSGLGLAFAGDHARARRLVEAEGELAVG
                     ATATHLGYRMRICYLGGDIEDALEAMPRASDVPAFPGW"
     gene            complement(1851..2495)
                     /locus_tag="PK264_21665"
     CDS             complement(1851..2495)
                     /locus_tag="PK264_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HRD78512.1"
                     /translation="MERAILELVERDAAALWWLGDRPAAALDIAMLSEAAAALRHIRM
                     SGQTRQTTLLDLTTDLGIAVVVALGTSADGGDLSIGLGCRPTLDEAARAAILELGQME
                     IGLLLAREKAAAGLPLEEGDRGHLARAALKIEMVIGGSAVAPQSTTEWARPIDPNALP
                     GIEVALVEHSMPGANIAVVQALALDLQPVTVDVVRPRLRRYQTTRFPRSTVALM"
     gene            complement(3046..3600)
                     /locus_tag="PK264_21670"
     CDS             complement(3046..3600)
                     /locus_tag="PK264_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRD78513.1"
                     /translation="MATTASGSFPALVLNADYRPLSYYPLSLWSWQDTVKAVFLDRVC
                     IVSEYDRYVRSPNVEMRIPSVVALKTYVKPALYPAFTRFNVFLRDRFSCQYCGCRSDL
                     TFDHMIPRSRGGQTRWDNVVTACAPCNLRKGGAMPDQAGMRPYQLPYRPTVYELHDNG
                     RAFPPNYLHESWTDYLYWDAELEP"
     gene            3829..>4127
                     /locus_tag="PK264_21675"
     CDS             3829..>4127
                     /locus_tag="PK264_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD78514.1"
                     /translation="MWRHLKSSLLALAALVPAAGFAVPEARAQSTLRIGLAEDPDVLD
                     PTLARTYVGRIVFASICDKLFDIDEKLNIVPQLALSHETSADGKAVTIKLRTGVK"
     assembly_gap    4128..4137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4138..5398
                     /locus_tag="PK264_21680"
     CDS             <4138..5398
                     /locus_tag="PK264_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRD78515.1"
                     /translation="SSPRASAASRHGAAMDAAAVKASLDRHISMTGSFRKPELAAVAS
                     VVVVDGATVRLNLSRPFSPLISQLADRAGMIMSPAATAAAGDKFGLKPVCAGPYRFVE
                     RVQQDRIVVEKFADYWDKGTVHIDRIIYRPIVDSTVRLANLRSGSLDLIERALATDLP
                     TIKSDAKLKLATQTEIGYQGLSLNIALGDAGKSGPFGDRRLRAALEAAIDRSALNEVV
                     FSGNFLPGNQWVSPKHPYYEPRFPVPGRDIAKAKRLVAEAGVKTPIAVDFMVPNNPES
                     RQVAELIQSMAKEAGFDMKIRVTEFATSLKEAEQGRYQAFLIGWSGRVDPDGNVFVFQ
                     KCGAPQNNRGFCDKDVDGWLEESRAKTAMADRRAAYAKIAERVLADGGIIYLYHRMVI
                     IAHSVKLEGYRQMPDGLVRVVGLKLKP"
     gene            5454..>5643
                     /locus_tag="PK264_21685"
     CDS             5454..>5643
                     /locus_tag="PK264_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78516.1"
                     /translation="MSGENLTNSIGKEQGLELFGEAKAKRRPRPGKSVREAAREVPVL
                     AETDILVVGGGPAGTAAAI"
BASE COUNT          958 a         1963 c         1804 g          908 t
ORIGIN      
        1 ttccagccgg gaaacgccgg cacatccgag gcccgcggca tggcctcgag cgcgtcctcg
       61 atgtcgccgc cgaggtagca gatgcgcatc cgatagccca agtgcgtggc cgtcgcgccg
      121 accgcgagct caccctccgc ctcgaccagc cgtcgcgcgc gtgcgtggtc gccggcgaac
      181 gcgagcccga gtccggacga gatcgtcgtc caaggatcat tgtcgttgag gcggagcgca
      241 tgctcataat gtcgcgcggc gagatcgaac tgctcgagca tcaggtgcga ccaggcgagt
      301 gacagctgcg cccgagagtc gccgcgatcg atgccgatcg cgcgccgcgc gaagtccagc
      361 gcctggctct cccccttggc atcgcgcatc aggccgtgat gcgcgagatg gatgatgttc
      421 tcgagctgcg cccggctcga gtacgcggga ccgaagtcgg gtgccgcctg gatgatctcc
      481 tcgaagatgc ggcgcgcctc gtgccagtcg tccctgcgga agctccggat cagctgctgg
      541 cccttcagcc actgatcgaa gatcagcggt gccgtatcgc gctggggaac gacgcgcgcg
      601 agccgcccct ctgaaatcga tacgttgagc gagcttgcga gctgccggac gacgctctgc
      661 tgcgccgtgg tccactcgcc gagcgagagc aacaggcgct cgctccacac gaactcgttg
      721 cttccccctt ccttcagagt gaagacgagg cgaagcccgt cccgggtttc gctcgcatgc
      781 gcctcgagtg cgtactcgtc agccgttgtt tcctcgcccg tcggcttgac accgtccctg
      841 acgtgccatt cccggaagag aatgagagag gcgatcagct cccggcgaaa gccgtcgcag
      901 agatagcgcc gctccggtga caccgcttcc atggcgagcg gcgcgaccat gatcacgagc
      961 ttgttcgtcc gcggctgaat gcgcaccacc gttggtgtcg tgatggggac caccggtccg
     1021 cccgcaaccg gagccgcagc gcactccctg atctcggcgg tgagacgccg cgtctccggt
     1081 gtgggaccag tgccgtgctc gcggacgagg acgtcctctt gcgcctttgc cacgcggagc
     1141 gccgccgtca ggtctccttg tgccgcatat gcgcgcatca gggcgcgcgt cgcctcatcg
     1201 tgcgtcggat cgagccgcag cagcgagcgc gcgatctcga tctggccgga ggtgcgccgc
     1261 gggtcgtaca attgccgctc gaggctgcgc aaaatgcggt ccgagatctt gctgcgctcg
     1321 ctcatcagcc aggtgtcgaa ctcctcgtcg agcccgtcga ggtcggcgag gaacgactgg
     1381 tcgacggcat ccggcgacaa gagccgttcg ggaacgacac cgccctccac gctctccagt
     1441 atctcggcga tgtcggtgcg cacgcgcgcc tggtcgagcc tcagctcgcg cgggtcgatc
     1501 ccgaggacgc cgcaacgcgc gagccggaac tggcgacgga gcacgccgac acaatggcgg
     1561 agcgactgct gcgcctggcg ctggggcgag ttgccccaga acatgccgac gagccgtcgc
     1621 cggctctcgc ggccatgatc ggcgagcgcc acataggcga tcatcgcctt ggccttctgg
     1681 gagcggatct tgatttgccg gccgccgaaa ctgatgtcca gccgtccgag cgtctcgatg
     1741 cgcaaaacag gatcggtctc gagacttgcc agcatgaact cgccccacgt cacgcgcggg
     1801 atcaagccgg ccacctgcgc cggctcgatc ctcgcccggg attgaggcta ctacatgagg
     1861 gccactgtcg agcgtggaaa acgtgttgtt tggtaacggc gcagccgcgg tcggacaacg
     1921 tcgaccgtga caggttgcag atcgagcgca agagcctgca cgacggcgat gtttgcaccg
     1981 ggcatcgaat gctcgacgag ggcgacctcg atgccgggca aggcgttcgg atcgatcggc
     2041 ctggcccact cggtcgtcga ttgcggcgcc acggcgctgc cgccgatcac catctcgatt
     2101 ttgagcgccg cgcgcgctag atgcccccta tccccttcct cgagcgggag gcccgccgcc
     2161 gctttctcgc gcgcgaggag cagcccgatc tccatctgcc cgagctcgag gatggcagcg
     2221 cgcgccgcct catcgagcgt cggccggcag ccgagcccaa tcgagaggtc accgccatcg
     2281 gcactcgtcc cgagcgcgac aaccaccgcg atgccgaggt cggtcgtgag atcgaggagc
     2341 gtggtctgcc gtgtctgccc cgacatgcga atgtggcgca gtgctgctgc ggcctccgac
     2401 agcatcgcga tgtcgagggc ggctgccggt cgatcaccga gccaccagag cgccgcggca
     2461 tcgcgctcca cgagctcgag gatcgcgcgc tccactgccc cgcggcaacg cctgaactca
     2521 acgggacgag tagcgggatg gcgcgccggt gcttcgcccg cctgaggcac cagtcggcgg
     2581 gcacgatcct cgctcgaccc gacgccgcgt cccgagcgct gacccaatca atcgtcgtgc
     2641 cgctcgagtt gaacggcgga tcggacagta cctcggcgac ggcacgcggg acatcgccct
     2701 ggcggtcggc cagccgccct cgccaagcga ccgccgcggg ccgctccacc tgagcggacc
     2761 tctccgccgc ctcgccaagg cacgccgcga gcgcatcctc gagcgcgagc ccagcgcctg
     2821 ccgtactgaa cgtgtgaccg ccttcagccg acgcctgccc gccgacgaac cgcaatccgg
     2881 gcgcccacgg cgagcggacg aggaacagcc ggcgcatcga tccaatcacc gagagaagtg
     2941 cacggccatg acgcgcagcc attgccgcag ccagcgcatc tcccggcgga gagcccagat
     3001 cgatgtcgac gaattcgagc ggcatgtgac ctcgctcgca cccgactacg gctcgagctc
     3061 tgcatcccag tagagataat cggtccaact ctcatgcagg tagttcggcg ggaacgcgcg
     3121 cccgttgtcg tggagctcgt acaccgtcgg ccggtaaggc agctggtacg gccgcatgcc
     3181 ggcctgatcg ggcatggcgc cgcccttcct gagattgcat ggcgcacatg ccgtcacgac
     3241 gttgtcccag cgcgtctgcc cgccgcgcga gcgcgggatc atgtgatcga acgtgagatc
     3301 gctccggcag ccgcaatact ggcaggagaa gcgatcgcgc aggaatacgt tgaagcgtgt
     3361 gaaggccggg tagagcgccg gtttcacata ggtcttcagc gcgacgacgc tcggaatgcg
     3421 catctctaca ttcgggctgc gcacgtagcg atcgtactcg gagacgatgc acacgcgatc
     3481 caggaacacg gccttcaccg tgtcctgcca gctccaaagc gaaagcggat agtagctgag
     3541 cggccgatag tcggcgttca gcacgagtgc cgggaagctt cccgatgcag ttgtagccat
     3601 tgctgtcacg agaccgattt cctgacagtt ccctgtccga atcgcactcg gagcctctcc
     3661 tcagcgctgg catactagct gtcggcgaca gtgctgtgaa gtccttgtgg acaaccggat
     3721 ttcagcagcc gccgacgctg ctctccccgc aatcgccatt gcgattgcag gaactgcgcg
     3781 cgccatctac gttcgctttc catatgggga gcggaaacgg agaattccat gtggcgccac
     3841 ctgaaatcgt cattactcgc acttgctgcc ctcgttccgg ccgcggggtt cgccgtgccc
     3901 gaggcacgtg cacagagcac cctgcgtatc ggcctcgccg aggacccgga tgtgcttgat
     3961 cccacccttg cgcgcaccta cgtcggccgc atcgttttcg catcgatctg cgacaagctc
     4021 ttcgacatcg acgagaagct caacatcgtg ccgcagctcg ccttgagcca cgaaacgtcc
     4081 gccgacggca aggctgtgac gatcaagctc cgcacgggcg tcaagttnnn nnnnnnngtc
     4141 ctcgccgcgg gcctcggcag cctcgcgcca cggcgcggcg atggacgcgg ctgccgtcaa
     4201 agcgagcctc gaccgccaca tctccatgac gggctcattc aggaagcccg agctcgcagc
     4261 cgtcgcgagc gtcgtcgtcg tcgatggggc aaccgtgcgg ctcaatctct cgcgcccgtt
     4321 ctcgccgctg atctcgcagc tcgccgaccg cgccggcatg atcatgtcgc ccgccgcgac
     4381 ggcggctgcc ggtgacaagt tcggcctgaa gcccgtctgc gccggcccct accgcttcgt
     4441 cgagcgcgtg cagcaggatc gcatcgtcgt cgagaagttt gcggactact gggacaaggg
     4501 caccgtccac atcgaccgca tcatctaccg gccgatcgtc gattcgaccg tgcgcctcgc
     4561 caacctgcgc tcgggctcgc tcgacctcat cgagcgcgcg ctcgcgaccg acctccccac
     4621 tatcaagtcc gacgccaagt tgaagctcgc gacccagact gagatcggct atcagggcct
     4681 ctcgctcaac atcgcccttg gagacgccgg caagtctggt cccttcggag accggcggct
     4741 gcgcgcagcc ctcgaggcgg cgatcgaccg ctcggccctc aacgaggtcg tcttcagcgg
     4801 caattttctc cccggcaacc agtgggtctc gccgaagcac ccctactacg agccgcgctt
     4861 cccggtgccc ggccgcgaca tcgccaaggc caagcgcctc gtcgccgagg ccggcgtcaa
     4921 gacgccgatc gcggtcgact tcatggtgcc gaacaaccct gagagccggc aggtcgccga
     4981 actcatccag tcgatggcga aggaagccgg cttcgacatg aagatccgtg tcaccgagtt
     5041 cgcaacctcc ttgaaggagg ccgagcaggg ccgctaccag gccttcctca tcggctggag
     5101 cgggcgcgtc gatccggacg gcaacgtatt cgtcttccag aagtgcggcg cgccgcagaa
     5161 caatcgcggc ttctgcgaca aggacgtcga cggctggctc gaggagtcgc gggcgaagac
     5221 ggcaatggcc gaccggcgtg ccgcgtacgc caagattgcc gagcgcgtgc tcgcagacgg
     5281 cggcatcatc tatctctacc atcgcatggt catcatcgcg cactcggtga agctcgaagg
     5341 ctaccgtcag atgccggacg gcctcgtgcg cgtcgtcggc ctcaagctga aaccgtgatc
     5401 cttggggcgg gcagccacgg gcccgcccat tccaatgctg acggagcgtt gccatgtccg
     5461 gcgagaacct caccaacagt atcggcaagg agcaggggct tgagctcttc ggtgaggcca
     5521 aggccaagcg gcgcccgcgg cccggaaagt ccgtccgcga ggcggcgcgc gaggtgcccg
     5581 tgctcgccga gaccgacata ctcgtcgtcg gcggcggccc tgccggcact gcggccgcga
     5641 tcg
//
LOCUS       DAORNH010000303         5592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10726_length_5592_cov_2.857323, whole genome shotgun sequence.
ACCESSION   DAORNH010000303 DAORNH010000000
VERSION     DAORNH010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10726_length_5592_cov_2.857323"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>559)
                     /locus_tag="PK264_21690"
     CDS             complement(<1..>559)
                     /locus_tag="PK264_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRD78517.1"
                     /translation="LKVPRMGAKTFEQAAGFLRIMNGENPLDASAVHPEAYPLVEKIA
                     KSAGRPLKSLIGDKTFLKTVKPAAYADGTFGVPTVTDIIAELEKPGRDPRPEFKTASF
                     KDGVEKLDDLKPGMQLEGVVTNVTAFGAFVDVGVHQDGLVHISELSDRFVTDPREVVK
                     AGDVVKVRVKEVDVARKRIALTMRSG"
     assembly_gap    560..602
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<603..2150)
                     /locus_tag="PK264_21695"
     CDS             complement(<603..2150)
                     /locus_tag="PK264_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRD78518.1"
                     /translation="MDKINQRIATELNVNVTQVTAAVELLGEGSTVPFIARYRKEKTG
                     GLDDTQLRKLEERLGYLRELEDRRAAVIRSIEEQGKMTPELARSLAAADSKVTLEDIY
                     APYKPKRRTKAMIAREAGLEPLTDALIANPALNPEVEAAKFINAEKGVADAKAALDGA
                     RAILIERIGEKAELVGMLREWLWDVGMITSKVVKGKESEGAKFSDYFDFNQRVKEIPS
                     HRALALLRGRNENILEVELDIVREEGKQHPAEGKIMVAMGFADKGRPTDKWLMDTIRL
                     AWKARLHVSLAVDLLTRTKERADGEAISVFSKNLKDLLLAAPAGPRVTMGLDPGIRTG
                     VKVAVVDATGKVLDTTTIYPHEPRRDWQGSLASLAALCAKHKVDLIAIGNGTASRETD
                     KLVADLVKAFPGMKITKVMVSEAGASVYSASELAAKEFPDLDVSLRGAVSIARRLQDP
                     LAELVKIDPKAIGVGQYQHDVDQSGLARSLDAVVEDCVNSVGVDVNTASAPLLARVSG
                     LNQTLAQN"
     gene            2451..3998
                     /gene="phaZ"
                     /locus_tag="PK264_21700"
     CDS             2451..3998
                     /gene="phaZ"
                     /locus_tag="PK264_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HRD78519.1"
                     /translation="MLYQWYELGHAAFRPARVAAGSARMLLSNPFNPLTHTPAGRHAM
                     AALEVFERTTRRYARPEFGITHTMIGGEKVPIVEEIIWERPFCRIVRFRRMTDERRTA
                     ADPRILLVAPMSGHFATLLRGTVETLIPDHDVYITDWQDARNVPLIAGRFDLDDYIHY
                     VEAMLRHFEGDVHVFAVCQPSVPVMAAIASMEADNDPAVPRTVTLAGGPIDTRHNPTV
                     VNALAEKRGIDWFQNNVVTSVPWPCRGFGRMVYPGFLQLSGFMTMNLDRHLAAHKDLF
                     LHLVRGDGDSAEKHREFYDEYLAVMDLTAEFFLQTVETVFIRHALPKGEMIHRGRRVD
                     LGAIRRVPLMTIEGEKDDITGLGQCKAAHLLCRNIPEDDRVHFECPHVGHYGIFNGSR
                     FRSQIAPRIAQFARTYDHRLGEARAHIGWVPPSVAASPSRTPGPIDASAVAFTFANEA
                     GDHVAAERQPAKLEANDNAAPAPTSVGGSAVAVAMGAQFAQLRLLGAAGSMLLDNVAR
                     VSARSAG"
     gene            4476..>5592
                     /locus_tag="PK264_21705"
     CDS             4476..>5592
                     /locus_tag="PK264_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRD78520.1"
                     /translation="MSRIPDFTSIGFDVAGRDGSKVPAFAASNMVTPEGIAIKPRYSA
                     ADVRGLDFVDGYPGIAPFLRGPYPTMYVTQPWTIRQYAGFSTAEASNAFYRRNLAAGQ
                     KGLSIAFDLATHRGYDSDHPRVRGDVGMAGVAIDSILDMRTLFEGIPLDRMSVSMTMN
                     GAVLPVLALFIVAGEEQGVAPEKLSGTIQNDILKEFMVRNTYIYPPAPSMRIISDIFA
                     YTSRRMPKFNSISISGYHMQEAGATADLELAYTLADGVEYARAGMAAGLDIDAFAPRL
                     SFFWAIGMNYFMEIAKMRAARLLWAKLIQENFKPKDSRSLSLRTHCQTSGWSLTAQDV
                     FNNVTRTCIEAMAATQGHTQSLHTNALDEAIALPTDFS"
BASE COUNT          923 a         1837 c         1752 g         1037 t
ORIGIN      
        1 cgcccgagcg catggtgagc gcgatgcgct ttcgggccac gtcgacctcc ttgacgcgca
       61 ccttgacgac atcgcccgcc ttcacgacct cacggggatc cgtcacgaag cggtccgaca
      121 gctccgagat gtgcacgagc ccgtcctggt gcacgccgac atcgacgaag gcgccgaaag
      181 ccgtgacgtt tgtgacgacg ccctcgagct gcatgccggg cttcaggtcg tcgagctttt
      241 cgacgccgtc cttgaagctc gcggtcttga actcggggcg cggatcgcgg cccggcttct
      301 cgagctcggc gatgatgtcg gtgaccgtcg gcacgccgaa cgtgccatcg gcataggcgg
      361 cgggcttcac ggtcttcagg aaggtcttgt cgccgatcag tgatttgagc gggcggcccg
      421 cagacttggc gatcttctcg acgagtggat aggcttccgg gtgtacggcg gaggcatcga
      481 gcgggttctc gccgttcatg atgcgcagga agcccgccgc ctgctcgaag gtcttggcgc
      541 ccatgcgcgg caccttgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nngttctgcg cgagcgtctg gttgaggccc gagacgcgcg cgagcagcgg cgctgacgcc
      661 gtgtttacat cgacgccgac cgagttcacg cagtcctcga cgacggcatc gagcgagcgg
      721 gcgagacccg actggtcgac gtcgtgctgg tactggccga cgccgatcgc cttcggatcg
      781 atcttgacca gttcggcgag cggatcctgg aggcggcgcg cgatcgagac ggcgccgcgc
      841 agcgacacgt cgaggtcggg gaactccttg gccgcgagct cggaggccga atagacagag
      901 gcacccgctt ccgacaccat caccttggtg atcttcatgc cggggaacgc cttgacgaga
      961 tcggcgacga gcttgtccgt ctcgcggctc gccgtgccgt tgccgatggc gatgaggtcg
     1021 accttgtgct tggcgcagag cgccgcgagg ctcgcgaggg agccctgcca gtcgcgccgc
     1081 ggctcgtgcg ggtagatcgt cgtcgtgtcg aggaccttgc ccgtggcatc gacgacggcg
     1141 accttgacgc cggtgcggat gccgggatcg agacccatgg tgacgcgtgg tcccgcggga
     1201 gccgccagca gcaggtcctt caggttcttc gagaagacag agatcgcctc gccgtcggcg
     1261 cgctccttgg tgcgcgtcag gaggtcgacc gcgaggctca cgtgcagtcg tgccttccag
     1321 gcaaggcgga tcgtgtccat cagccacttg tcggtcgggc ggcccttgtc ggcaaatccc
     1381 atggcgacca tgatcttgcc ttcggcggga tgctgcttgc cctcctcgcg cacgatgtcg
     1441 agctcgacct cgaggatgtt ctcgttgcgg ccgcgcagca gtgcgagcgc gcggtgcgag
     1501 gggatctcct tcacgcgctg attgaagtcg aaataatccg agaacttggc gccctcgctc
     1561 tccttgccct tgaccacctt cgaggtgatc atgccgacat cccagagcca ctcgcgcagc
     1621 atgccgacga gctcggcctt ctcgccgatg cgctcgatca ggatggcgcg ggctccgtcg
     1681 agggcggcct tggcgtcggc aacgcccttc tcggcattga tgaacttcgc ggcctctacc
     1741 tcgggattga gcgcgggatt ggcgatgagc gcatcggtga gcggctcgag gccggcttcg
     1801 cgcgcgatca tcgcctttgt gcggcgcttc ggcttatagg gcgcgtagat gtcctcgagc
     1861 gtcaccttgc tgtcggcggc cgcgagcgag cgggcgagct caggcgtcat cttgccctgc
     1921 tcctcgattg agcggatgac ggctgcgcgc cgatcctcga gctcacggag atagccgagc
     1981 cgctcctcga gcttgcgcag ctgcgtgtcg tcgagcccgc cggtcttttc cttgcggtag
     2041 cgcgcgatga acggaacagt cgatccctcg ccgagcagct ccaccgccgc cgtgacctgc
     2101 gtcacgttga cattgagctc tgttgcgatg cgctgattga tcttgtccat tcgtcgcctc
     2161 gatcgttctg acacgggacg ccaactgttc gcgtcctcac ctatcaccga ctggcacgcg
     2221 actgaagcgc gtggccattt gatcaacgat tttccgaatg caatgtcgcg ggcagctggc
     2281 accttcgatg ggcaccgcaa cattggcctt tgcgggtgca gcgaatcggg gcaggctgac
     2341 ctggggacaa ctaccattgt cgggttgccg gcgaccgcga ctcacatgcc tgaagaagta
     2401 gcgcagagcg gttgatcggc ccccgcgaat gacattttgg gggatgccgt atgctctacc
     2461 agtggtacga gctcggccac gctgcctttc gtccggcgcg ggtcgcagct ggctcagcac
     2521 gcatgttgct gtccaacccg ttcaatccgc tgacacatac gccagccggt cgccacgcca
     2581 tggccgcgct cgaggtgttc gagcgcacga cgcggcgcta tgctcggccc gagttcggca
     2641 tcactcacac gatgatcggc ggcgagaagg tgccgatcgt cgaggaaatc atctgggaac
     2701 ggccgttctg ccgtatcgtg cgattccgcc gcatgaccga cgagcgtcgc actgccgccg
     2761 atccgcggat cctgctcgtc gcgccgatgt ccggtcattt cgcgaccctg ctgcgcggca
     2821 cggtcgagac gctcattccg gaccacgacg tctacatcac cgactggcag gacgcgcgca
     2881 atgtgccgct gatcgccggc cgcttcgatc tcgacgacta catccactac gtcgaggcga
     2941 tgctgcgcca cttcgagggc gacgtgcacg tcttcgccgt ctgccagccg tcggtgccgg
     3001 tgatggcggc catcgcctcc atggaggccg ataatgatcc ggccgtcccg cgcacagtca
     3061 cgctggccgg cggtccgata gatacgcggc acaatccgac cgtcgtcaac gctctcgccg
     3121 agaagcgcgg catcgactgg ttccagaaca atgttgtgac gagcgtgccg tggccgtgcc
     3181 gcggcttcgg gcgaatggtg tacccgggct tcctgcagct ctcgggcttc atgaccatga
     3241 acctcgaccg gcacttggcg gcgcacaagg acctgttcct gcatctcgtg cgcggcgatg
     3301 gcgattcggc ggagaagcac cgcgaattct acgacgagta tctcgcggtg atggacctca
     3361 ccgctgagtt cttcctgcag accgtcgaaa cggtgttcat ccgccatgcg ctgccgaagg
     3421 gcgagatgat acatcgcggg cgccgcgtcg acctcggcgc gatccggcgc gtgccgctga
     3481 tgacgatcga gggtgagaag gacgacatca ccgggctcgg ccagtgcaag gcggcgcatc
     3541 tcctgtgccg caacatcccc gaggacgacc gcgtccactt cgagtgcccg cacgtcggcc
     3601 actacggcat cttcaatggt tcgcggttcc gctctcagat cgcgccgcgc atcgcccagt
     3661 tcgcgcgcac ctatgaccat cgcctgggcg aggcgcgcgc tcacatcggc tgggtgccgc
     3721 catcagttgc agcgtccccg agccgcacgc cggggccgat cgacgcgagt gcggtcgcct
     3781 tcacgtttgc caacgaggcg ggcgatcacg ttgccgccga acggcaaccg gcaaagctgg
     3841 aagcaaacga caatgcagcg ccggccccca cgtcggtcgg cggcagcgct gttgccgtag
     3901 ccatgggtgc ccagttcgcc cagctgcgtc tcctcggcgc tgccggcagc atgctcctcg
     3961 acaatgtcgc gcgtgtcagc gcgcgcagcg ccggctgacc cctcgctgtc ggtattcgca
     4021 cgctggaaag ttcggcctca cacgagtgcg tgtggggccg aaagttgtgt catatcagca
     4081 acatttgcgg tcgcatcgca cgtcgttaac cattcgttaa tcattgtggc gatatgcttc
     4141 gacatggttc gattctcgga tcgagcctca gaaagagccg tcagctgccc agagcggctc
     4201 gatcgcaggc tctgaagcca aagagcctga tacgcattct actgcgtcga cattttgcag
     4261 acacaggtct tacagacaca ggtgtgtctg acattcgaca aacctctcga ccccagaagc
     4321 ggcccggtcg gtgcaacgca ccaccgggcc gcttcatttc taccggcctg ccgccaatca
     4381 ccgcaaccaa tgacgacctt cgttcgcagc cgtattggtg ctagacgagc aagtggagcc
     4441 gagaggttgg ccccgtcatc cgctggagta gccccatgag ccgcattcct gacttcacat
     4501 cgatcggttt cgatgtcgcg ggacgcgacg gttcgaaggt gccggctttc gcggctagca
     4561 acatggtgac gccggaaggc atcgcgatca agccacgcta tagtgccgcc gacgtcaggg
     4621 gactagactt cgtcgacggc tatccgggca tcgcgccttt ccttcgcggt ccctatccga
     4681 cgatgtacgt cacccaacct tggacgatcc gccagtacgc aggtttctct acggccgaag
     4741 cctcgaacgc cttctaccgg cgcaacctcg ccgcgggcca gaagggcctc tccatcgcct
     4801 tcgacctcgc gacgcatcgc ggctacgaca gcgatcatcc gcgggttcgc ggcgatgtcg
     4861 gcatggcggg cgtggcgatc gattcgattc tcgacatgcg cacgctgttc gagggcatcc
     4921 cgctcgaccg catgtccgtt tccatgacca tgaacggcgc cgtgctgccg gttctcgccc
     4981 tcttcatcgt ggcgggcgag gagcagggcg tcgcacccga aaagctctcg gggaccattc
     5041 agaacgacat tctgaaggag ttcatggtcc gcaataccta catctacccg cctgcgccct
     5101 cgatgcggat catctccgac atcttcgcct acacctcgcg ccggatgccc aagttcaact
     5161 cgatctcgat cagcggctat cacatgcagg aggccggagc gacggccgac ctcgaactcg
     5221 cctacacgct cgccgacggc gtcgagtatg cccgcgccgg catggccgct ggcctcgaca
     5281 tcgatgcctt tgcgccacgc ctctcgttct tctgggcgat cggcatgaac tacttcatgg
     5341 agatcgccaa gatgcgggcc gcgcggctcc tctgggcgaa gctcatccag gagaatttca
     5401 agccgaaaga cagccggtcg ctgagcttga ggacccattg ccagacctcc ggctggagcc
     5461 tcacggcgca ggacgtgttc aataatgtga cgcgcacctg catcgaggcc atggcggcga
     5521 cgcagggcca tacgcaatcg ctgcacacca acgccctcga cgaggcgatc gcgctgccga
     5581 ccgacttctc gg
//
LOCUS       DAORNH010000304         5551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10833_length_5551_cov_2.805131, whole genome shotgun sequence.
ACCESSION   DAORNH010000304 DAORNH010000000
VERSION     DAORNH010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10833_length_5551_cov_2.805131"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PK264_21710"
     CDS             <1..1451
                     /locus_tag="PK264_21710"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD78521.1"
                     /translation="AVTKPAATGLAAPAPPAPSAQDTADCRGSDNAKAVAACTRIIEY
                     GTRQPAAVLADAYHFRGLAHGRAGNHDIAIADFSEALRVRTGYGPSYASRGVAYANRG
                     NSDAAFTDYSEAIRINPKDGVAWSNRSGINLRRGNIDAAIADASEALRIRPNHIGALI
                     NRAEAWQKKGDAAKARADISAALAMTASTDFERRRLNDARNILASLDAAAAKPAAPVT
                     GPTSTATPRPVTPPPVASAAPPPAYDATKPQRRVALVVGIGAYASAAHLEHPQSDARE
                     FASALRRLGFDDVIERRDATLAELEADLKSFGDLAAVADWAVLYFSGHILQVDGRNHL
                     LPADAKLARIGHLADETLDLERILDKVREARAIKLVILDACRVNPFAANLAKASGGRS
                     IKTGIAAVAPKGNEVISVCGSDGQVIDDGDGRHAPFTRELLGLIEDPNMDLARLLNGA
                     GQALARATAARALPKLYGVLPSGSIPLKTGAR"
     gene            1582..2667
                     /locus_tag="PK264_21715"
     CDS             1582..2667
                     /locus_tag="PK264_21715"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HRD78522.1"
                     /translation="MAKAAKAKPNTYRIAVIPGDGIGNEVVPEGIKVLEAVGRKFDIA
                     FQWDNFGWSCETFKKTGKMMPDDGLDQIRRHDAIYLGAVGFPGVPDHVSLWGLLIPIR
                     RNFQQYINLRPVRLFEGVPCPLAGRKPGDIDYWIVRENNEGEYSSIGGRMNEGSEYEM
                     AIQQAVFTRRGCDRVMRYAFNLAATRKRNEVVSATKSNGISISMPYWDERFAAISKEF
                     PKAKTSQYHIDILTAHLVRNPQWFDVIVGSNLFGDILSDLGPATTGTIAIAPGGNINP
                     EKDYPSMFEPVHGSAPDIAGKGISNPIGQIWSGAMMLEHLGHKDAADAIVKAIEKLLR
                     DSDVRTRDMGGKAMCKELGDAIVELVK"
     gene            complement(2693..3430)
                     /locus_tag="PK264_21720"
     CDS             complement(2693..3430)
                     /locus_tag="PK264_21720"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRD78523.1"
                     /translation="MSEPLYDSIGAGYQRQRVPDPRIAALILAALGDARSVLNVGAGT
                     GSYEPTDREVLAVEPSRLMISQRPGGAAPCLVGSAEALPVTDQSVDAAMAILTMHHWS
                     NWRRGVAELRRVARRRVVILTFDPTGPWFWLTEYMPFIAEDDRRIMPPLAEIAEALGG
                     AVITPVPVPHDCIDGFLGAYWRRPEAYLDPAVRGSISSFSKFDASAGLARLDADLASG
                     AWRARHGALTRRESLDIGYRLIRAELA"
     gene            3527..3898
                     /locus_tag="PK264_21725"
     CDS             3527..3898
                     /locus_tag="PK264_21725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HRD78524.1"
                     /translation="MAIHAPVPILRMFDVVRTRDFYIGYLGFRIQFEHRFEPTLPLYM
                     GIRRDRCELHLSEHHGDATPGSALRIEVDDIDGLNAELAAKGYGFARPRIEPRPWGTR
                     EMTITDPSGNRITFAAVETQK"
     gene            4007..4660
                     /locus_tag="PK264_21730"
     CDS             4007..4660
                     /locus_tag="PK264_21730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HRD78525.1"
                     /translation="MIRKVHTCIVLDRSGSMEDCRTDAIGAVNSFLRQTKDDTTSDNR
                     ISLIIFDSQSIDVIRDRVPAAVCKDLQPGEYEPRAGTPLLDAVGHGASVLSKSAEKEE
                     RCVLAVMTDGHENSSREYTYGTLKALLERRQKEDGWLVVYLGAGHDSWAQADKLGFAR
                     GNVASFDKRSLHATSVELKKRRQRYVDAGDHVLEARFGGFTDEERAAMQGGDDRKGA"
     assembly_gap    4771..4780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4813..>5183
                     /locus_tag="PK264_21735"
     CDS             4813..>5183
                     /locus_tag="PK264_21735"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HRD78526.1"
                     /translation="MPRRATGEEAIIQGYLAPLAAGFPGAFGLRDDCAALTPAPGHDL
                     VLKTDAIAEGVHFRPDDPPEDIGWKALAVNVSDLAAKGATPRIYLLSLAFPTFPDDDW
                     LSGFVEGLGQAQAAFGITLAGG"
     assembly_gap    5184..5227
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5228..>5551
                     /locus_tag="PK264_21740"
     CDS             <5228..>5551
                     /locus_tag="PK264_21740"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRD78527.1"
                     /translation="EVRTVRMVRRTSARPGDVILVSGTLGDSAAGLGVLGGAAEGLDE
                     VERAALVQRYHRPMPRLGLRTALAHASAAMDISDGLAKDLARMCRASGEIGARIEAAR
                     LPLSPS"
BASE COUNT          962 a         1851 c         1814 g          870 t
ORIGIN      
        1 cagccgtgac caagccggct gcgaccggcc tggcagcacc cgctccgccg gcaccgagcg
       61 cgcaggatac agccgattgc cgcggcagcg acaacgccaa ggcggttgcc gcctgcacac
      121 ggatcatcga gtacggcacg cgccagccgg cagccgtgct cgccgatgcc taccatttcc
      181 gcggccttgc gcatggccgt gcgggcaacc atgacatcgc gattgccgat ttctcggagg
      241 ctttgcgggt gaggacgggc tacgggccga gctatgcgag ccgtggcgtc gcatatgcca
      301 accgcggcaa ctcggacgcg gccttcaccg actacagcga ggcgatccgc atcaatccaa
      361 aggacggggt ggcctggtcc aaccgctccg gcatcaatct ccggcgcggc aacatcgacg
      421 cggcgattgc ggatgcgagc gaggcgctgc gcattcgccc caatcacatc ggtgcgctga
      481 tcaaccgggc ggaggcctgg cagaagaagg gcgatgcggc gaaggcccgc gccgacatct
      541 cggccgcact cgcaatgacg gccagtaccg acttcgagcg ccgccgcctc aatgatgcgc
      601 gcaacattct cgcgagcctg gatgcggctg cggccaagcc ggcggcgcct gttacgggcc
      661 cgacgagtac ggcaacgccc aggccggtaa cgccgccacc cgtggcatcg gcggcgccgc
      721 ctcccgccta tgatgcgacg aagccgcagc gccgcgtcgc gctcgtcgtc ggcatcggcg
      781 cctatgcgtc tgccgcgcat ctcgagcacc cgcagtcgga cgcgcgagag tttgcctcgg
      841 cgctccgacg cctcggattc gacgacgtga tcgagcggcg cgacgcgacg ctcgccgagc
      901 tcgaggccga cctcaagagc ttcggtgatc tcgcggcggt ggccgattgg gctgtccttt
      961 atttcagcgg gcacattttg caggtcgatg ggcgcaacca tctcctgccg gcagatgcca
     1021 agcttgcgcg tatcgggcac ctcgccgacg agacgctcga tctcgagcgc attctcgaca
     1081 aggtgcgcga ggcgcgcgcc atcaagctcg tcatcctcga cgcctgccgc gtgaacccct
     1141 ttgccgcaaa tctcgccaag gcgagcgggg ggcgctcgat caagacggga attgcggccg
     1201 ttgcgcccaa gggcaacgag gtcatatcgg tgtgcggcag cgacggtcag gtgatcgacg
     1261 acggcgacgg acgccatgcc ccgttcacgc gcgagctgct tggcctaatc gaggatccga
     1321 atatggacct ggcacgcctt ctcaatggtg ccggacaggc gctggcccgc gcgacggcag
     1381 cgagggcgct gcccaagctc tacggcgtgt tgccgagcgg cagcatccct cttaagaccg
     1441 gcgcgcgata ggcgcagccg gcgggcggtt tggactttcc cggccggcat ggctggggcg
     1501 cgccgggccc gaccgcctgg acggcgcgcc ctcgtttgtg gcacgcctct ccggcagttt
     1561 cgtcgcgctg gaggaaacac catggccaag gccgcgaagg caaagcccaa tacctaccgt
     1621 atcgccgtca ttccgggcga cggcatcggc aacgaggtcg tcccggaagg catcaaggtg
     1681 ctcgaggcag tcggccgcaa attcgacatt gccttccaat gggacaactt cggctggtcg
     1741 tgcgagacct tcaagaagac cggcaagatg atgccggacg acggcctcga ccagattcgg
     1801 cgtcacgacg ccatctatct cggcgccgtc ggcttcccgg gcgttcccga ccacgtctcg
     1861 ctctggggcc tgttgatccc gatccgccgc aacttccagc agtacatcaa cttgcgcccc
     1921 gtgcgcctct tcgagggcgt gccgtgtccg cttgctggca gaaagcccgg cgacatcgac
     1981 tactggatcg tgcgcgagaa caacgagggc gagtactcct ccatcggcgg gcgcatgaac
     2041 gaaggctccg aatacgagat ggcgatccag caggccgtct tcacgcgtcg cggctgcgac
     2101 cgcgtcatgc gctacgcctt caatctcgcg gccacccgaa agcgcaacga ggtcgtctcg
     2161 gcgaccaaat cgaacggcat ctcgatctcg atgccctact gggacgagcg cttcgccgcg
     2221 atctcgaagg agttcccgaa ggccaagacc agccagtacc acatcgacat cctgaccgcg
     2281 cacctcgtgc gcaatccgca gtggttcgac gtgatcgtcg gctcgaacct cttcggcgac
     2341 atcctctccg acctcggccc cgcgacgacc ggcaccattg ccatcgcgcc cggcggcaac
     2401 atcaacccgg agaaggacta tccctccatg ttcgagccgg tgcatggttc ggcccccgac
     2461 atcgccggga agggcatctc gaacccgatt ggccagatct ggtctggcgc catgatgctc
     2521 gagcatctgg gccacaagga cgccgccgac gccatcgtca aggcgatcga gaagctgctc
     2581 cgtgactcgg acgtgcgcac ccgcgacatg ggcggcaagg ccatgtgcaa ggagctcggc
     2641 gacgctatcg tggaacttgt gaagtaggca tgttcggcgt gcggcggcag ggtcacgcga
     2701 gctcggctct gatcagacga tagccgatgt cgaggctctc gcgacgcgtg agggcgccat
     2761 gccgcgccct ccatgcgccg ctcgcgaggt cagcatcgag ccgtgcgagc cccgcgctcg
     2821 catcgaactt cgagaacgag gagatcgagc cgcgcactgc cgggtcgaga taggcctcgg
     2881 ggcggcgcca gtaggcgccg aggaagccgt cgatgcagtc gtgcggcacc ggcacgggcg
     2941 tgatcacggc gcctccgagt gcttcggcga tctcggcgag cgggggcatg atccgccggt
     3001 cgtcctcggc gatgaacggc atatactccg tgagccagaa ccatgggccc gtcgggtcga
     3061 acgtcaggat cacgacgcgg cggcgcgcta cgcggcggag ttccgccacg ccccggcgcc
     3121 agttgctcca gtgatgcatg gtcaggatcg ccatggcggc gtcgacgctc tggtccgtga
     3181 cagggagtgc ttcggctgaa ccgacgaggc agggcgcggc gccgcccggc cgctgactga
     3241 tcatcaggcg cgaaggctcg acggcgagaa cttcacggtc ggtcggctcg taggaacccg
     3301 tcccggcgcc gacgttgagc accgagcgcg catcgccgag tgctgccagg atgagtgcgg
     3361 cgatccgcgg atcgggcact cgctggcgtt ggtagcccgc gccgatgctg tcatagagag
     3421 gttcgctcat cagaagcacc tatggcacaa gctgtcgagg ttgccatgcg gagaggcacg
     3481 ctcggtgcgg tccggttgag gtgcaagcca acaaggggag atcccaatgg ccatccatgc
     3541 tcccgttccc atcctcagga tgttcgacgt cgtccggaca cgcgacttct atatcggcta
     3601 cctcggattt cgcatccagt tcgagcatcg cttcgagcct acgcttcccc tctacatggg
     3661 gatcaggcgc gaccgatgcg agctgcatct ctccgagcat cacggcgatg ccacgccggg
     3721 ctcggcgctg cgcatcgagg tcgatgatat cgatgggctg aatgcggagc tcgctgcaaa
     3781 aggctacggt ttcgcccgtc cgcggatcga gccgcggccc tggggaacgc gcgagatgac
     3841 gatcaccgat ccgagcggca accgcatcac gttcgcggcc gtcgagacgc aaaaataaat
     3901 gcagaatcag acgcttctag gccaattgac accccgtcgc cacaatcgct tcccatctgc
     3961 ttgaagctgg cagagaggcc agcgtgacgc ttgtgggaca tccataatga tcagaaaagt
     4021 ccatacctgc atcgttctcg atcgctctgg ctccatggag gattgccgga ccgacgccat
     4081 cggcgcggtg aacagttttc tcaggcagac caaggacgat accacctccg acaatcgcat
     4141 cagcctaatc atattcgaca gccaatcgat cgatgtgatc cgcgaccgcg tacccgccgc
     4201 cgtctgcaag gacctgcaac ccggagaata cgagccccga gccgggaccc cgctcctcga
     4261 tgccgttggc catggcgcga gcgtgctctc gaagtcggcc gagaaggaag agcgctgcgt
     4321 cctcgccgtc atgacggacg gccacgagaa ctcgtcgcgc gagtacacgt acgggacgct
     4381 gaaggcactg ctcgagcgcc gccagaagga ggacggctgg ttggtcgtct acctcggcgc
     4441 gggtcacgac agctgggcgc aggcggacaa gctcggcttt gcgcgaggta atgtcgcgtc
     4501 cttcgacaag cgcagccttc acgcgacctc cgtcgagctc aagaagcgcc ggcagcgcta
     4561 tgtcgatgct ggagaccacg tcctggaggc gcgcttcggc ggcttcacgg acgaggagcg
     4621 cgccgccatg caaggtggcg atgaccgcaa gggcgcctga agcgcggcga acattcgaga
     4681 acaatgccgg cggcgcgagc aatcgcgccg ctgtcattgg gtgccggcga gcggcgtgat
     4741 aggtgttgcc gacccgctcg ggctgagcgg nnnnnnnnnn cgcctccagg gcgctcgcca
     4801 aggaaacctc aaatgccgcg acgcgccacc ggcgaggaag cgatcatcca gggctatctc
     4861 gcgccgctgg cggcgggttt tcccggcgcg ttcggcctcc gcgatgattg cgccgcattg
     4921 acgccggcgc ctggtcacga cctcgtcctg aagaccgacg cgattgccga gggcgtgcac
     4981 ttcaggcctg atgatccgcc cgaggacatc ggttggaagg cgctcgcggt caacgtctcc
     5041 gacctcgccg cgaagggcgc gacaccgcgc atctatctcc tctcgctcgc tttcccgacg
     5101 ttccccgatg acgactggct ctcgggcttc gtcgagggtc tcggccaggc gcaggcggcc
     5161 ttcgggatca ctcttgcggg cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnngag gttcgtacgg tccgcatggt gcgccgcacg agcgcgcggc cgggcgacgt
     5281 gatcctcgtc agcggcacgc tcggggacag cgcggctggc ctcggtgtgc tcggcggagc
     5341 agcggagggt ctcgacgagg tggagcgcgc ggcgctcgtt caacgctacc accggccgat
     5401 gccgcggctt gggctgcgca cagcactggc tcatgcctcc gctgcgatgg acatctcgga
     5461 cgggctcgcc aaggacctcg cacgcatgtg ccgggcgagc ggggagatcg gcgcccgcat
     5521 cgaggccgcc cgcctgccgc tgagcccctc c
//
LOCUS       DAORNH010000305         5549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10839_length_5549_cov_2.591918, whole genome shotgun sequence.
ACCESSION   DAORNH010000305 DAORNH010000000
VERSION     DAORNH010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10839_length_5549_cov_2.591918"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PK264_21745"
     CDS             <1..727
                     /locus_tag="PK264_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HRD78528.1"
                     /translation="KADPPDLVDVQARKRALATFAANAPQRLVRLRAALRDGSYRPRA
                     LRRVAIPKDDGGERVLAIPAVVDRIAQTAIAQALTPWLEREFEDSSFGYRPGRSVQDA
                     VRRVSELRAAGLTHVVDADISAFFDSVPHESLLARLAESMDEGPTTQLIALWLEHAGN
                     DGRGLAQGSPLSPLLSNLCLDRIDEAFAGRGARIVRFADDFVILCRSHDAAGESWHVR
                     EADECRTVLYCIRLRELRNLTYW"
     gene            773..1462
                     /locus_tag="PK264_21750"
     CDS             773..1462
                     /locus_tag="PK264_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78529.1"
                     /translation="MDGASAFDLILSGDRALFAIVRLSLIVSLSATLLAALVGMPFGA
                     GLALSRFSGRGAVIATLNALMGLPPVVVGLAVFLLLSRAGPLGNLGILFTPTAMVIAQ
                     AILIAPIIAALTRQVLEDLWAEYRDELTAMEVGPVQRIATLLWDGRLALVTIGLAGFG
                     RAAAEVGAVMIVGGNIDGHTRTMTTAIALETSKGNLPLAMGLGLVLIVLILAVNAIAW
                     ALRQWGERHAG"
     gene            1452..2210
                     /locus_tag="PK264_21755"
     CDS             1452..2210
                     /locus_tag="PK264_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD78530.1"
                     /translation="MLDSASDASADVGPLLRAGRALPLVFERVGFTAGGTRVLTEVSL
                     TLAPGAPTFVLGPNGAGKTTLLKLAAGLHAPTTGAIRYAGLAKAPKGALAIMFQKPVM
                     LRRSAAANIAYALWAAGRSATVADITRLLELANIAPLSNRPARRLSGGEQQRLALVRA
                     LARDPEVLLLDEPTASLDPAATRLVEDIIRRIAASGVKIVMTTHDLGQARRLAGEAIL
                     FAGGRLVEQTAADRFFSGPSSEAARRFLTGELLG"
     gene            2228..3061
                     /locus_tag="PK264_21760"
     CDS             2228..3061
                     /locus_tag="PK264_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD78531.1"
                     /translation="MITKRLALVAAAAIGLSLAAAPLPADDKSIVVASTTSTQDSGLF
                     GHILPIFKARTGIAVKVVSQGTGQAIDTGKRGDADVVFVHAKPQEGKFVADGFGVKRY
                     DVMYNDFVLIGPKGDPAAIKGGRDILAALTTIHAKGAPFVSRGDKSGTHAAELALWKA
                     ASLDPAASKSAWYREIGQGMGAALNTAAAMGAYVLADRGTWLSFKNREALLIAVEGDR
                     RLFNQYGIILVNPAKHKHVKRELGQSFVDWIVSKEGQDAIAGFKINGEQLFFPNAGSA
                     K"
     gene            3155..3572
                     /locus_tag="PK264_21765"
                     /pseudo
     CDS             3155..3572
                     /locus_tag="PK264_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844081.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     assembly_gap    3573..3592
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3593..3717
                     /locus_tag="PK264_21770"
     CDS             <3593..3717
                     /locus_tag="PK264_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD78532.1"
                     /translation="GEAHPGLIDLDAAPRLAVLTVQAEALPAFSEISQPFLPPT"
     gene            3816..5171
                     /gene="tolB"
                     /locus_tag="PK264_21775"
     CDS             3816..5171
                     /gene="tolB"
                     /locus_tag="PK264_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HRD78533.1"
                     /translation="MTRRQTTAPTRRDVFRLAVGASALSAGVAAVGEDVRAQGKGPIR
                     VDVDSSSLRPRPIAIPIFLSEDSKLGAEVADIIAADLAGSGLFQPLDRSAFPPVRDFN
                     GPIQFADWRAGGAEALLTGRVSRLPDGRIDGAFRLWDVVLEKALDGQRVGTTPQSWRR
                     FAHIIADRVYEKMTLERGYFDTRVVFVDETGAKDRRVKRLAIMDQDGHNVGLLSDGRE
                     LVLTPRFSPTGQMIAFMSYAGQKPSVGTMNLATRERQLIGDLGGMTFAPRFSPDGQRL
                     VFSLQSEGSSSIVEMDLRSRQSRRLTQGPAIDTAPCFSPDGQSLVFESDREGNQQLYV
                     MAAGGGPARRLSYGEGRYSTPVWSPRGDTIAFTKQASGRFLIGVMRTDGSGERILTDG
                     FHNEGPTWAPNGRVLMFFREERGANAGPRIFSVDLTGRIERAIPTPSFGSDPAWSPLL
                     K"
BASE COUNT          877 a         1792 c         1898 g          962 t
ORIGIN      
        1 aaaggccgat ccgcccgatc tggtggatgt acaggcgagg aagcgcgcgc tggcgacgtt
       61 cgccgcgaac gccccacagc gcctggtccg gctgcgcgcc gcgctgcgcg acggaagcta
      121 ccggccacgg gcgctgcgcc gcgtcgccat ccccaaggac gacggcggcg agcgggtgct
      181 tgccattccg gcggttgtcg accgcatcgc ccagaccgcc atcgcccagg cgctgacgcc
      241 gtggctggag cgggagttcg aggacagctc gttcggctat cgccctggcc gatcggtgca
      301 ggatgccgtg cgccgcgtga gcgagctgcg cgcggcgggc ctgacgcacg tggtggacgc
      361 cgacatcagc gccttcttcg acagcgtgcc gcatgagagc ctgcttgccc gtcttgccga
      421 aagcatggac gaggggccga ccacccagct gatcgcgctg tggctcgagc acgcgggcaa
      481 cgacgggcgc ggcctcgcac aaggctcgcc gctgtcgccc ctgctgtcca acctctgtct
      541 ggaccggatc gacgaggcgt ttgccgggcg tggcgcgcgg atcgtgcgct ttgccgacga
      601 cttcgtgatc ctgtgccgct cacacgatgc ggcgggagag tcatggcatg tgcgcgaggc
      661 ggatgaatgc cgcactgttc tgtactgcat aaggctgcgg gaattacgaa acttgacata
      721 ttggtagaga gcgcgagatc tctcgtcggc aacagatgtc gagagaccgc acgtggacgg
      781 cgccagcgcc ttcgatctca tcctgtcggg cgaccgcgcg ctgtttgcga tcgtgcggct
      841 gtcgctgatc gtatcgctat cggcgacgct actcgcggct cttgtcggca tgccgttcgg
      901 tgcgggcctc gccctgagcc ggttctcggg ccgaggcgcg gtcatcgcga cgctgaatgc
      961 gcttatgggg ctgccgccgg tcgtcgtcgg gctcgccgtg ttcctgctgc tgtcgcgcgc
     1021 aggcccactc gggaacctcg gcatcctctt cacgccgacc gcaatggtca tcgcgcaagc
     1081 gatcctgatc gcgccgatca tcgcggcgct gacccggcag gtgctcgagg atttgtgggc
     1141 ggagtatcgc gacgagctca ccgcgatgga ggtcggcccg gtgcaacgca tcgcgactct
     1201 gctctgggac gggcgcctcg cgctcgtcac gatcgggctc gcgggcttcg gccgtgcggc
     1261 ggctgaggtc ggcgcggtga tgatcgtcgg cggcaacatc gacggccaca cgcgcacgat
     1321 gacgacggca atcgccctcg agacctccaa aggcaatctg ccgctcgcga tgggcctcgg
     1381 gctcgtcctg atcgtgctga tccttgcggt gaatgcgatt gcctgggcac tccggcagtg
     1441 gggagagcgc catgctggat agcgcaagcg atgcctcggc cgatgtgggg ccactgctgc
     1501 gtgcggggcg cgccttgcca ctcgttttcg agcgcgtcgg cttcacggct ggcggaacac
     1561 gcgtcctcac cgaagtcagt ctgaccctcg cccccggcgc gccgacattc gtgctcggtc
     1621 cgaacggcgc gggcaagacg acgctgctta agctcgcggc cggcctccat gcgccgacga
     1681 cgggcgcgat ccgctatgcc ggcctcgcca aggcgccgaa aggagcgctg gcgatcatgt
     1741 tccagaagcc cgtgatgctg cgccgctctg ctgccgccaa tattgcctat gcgctctggg
     1801 cagcgggccg gtccgcaaca gtggccgaca tcacgcgcct gctggagctc gcgaacatcg
     1861 ccccgctctc aaaccggcca gcacgacgcc tttcgggcgg cgagcagcag cggctcgccc
     1921 tcgtgcgcgc gcttgcccgc gatccggagg tgctgctgct cgacgagccg acggcaagcc
     1981 tcgatccggc ggcgaccagg ctcgtcgagg acatcatccg gcgaattgcc gcgtcaggcg
     2041 tgaagatcgt catgaccacg catgatcttg ggcaggcgcg ccgccttgcg ggcgaggcga
     2101 tccttttcgc gggcggacgt ctcgtcgagc agacggccgc cgaccgtttc ttctcgggtc
     2161 catcgagcga ggcggcgcgt cgcttcctca ccggtgagct tctcggctga agccacagga
     2221 gacactgatg atcaccaagc gtctggcact cgtcgccgca gccgcgatcg gactatcgct
     2281 cgcagccgct ccgctgcccg ccgacgacaa gtcgatcgtc gtcgcctcga ccacatcgac
     2341 gcaggattca ggtctcttcg gccacatcct gccgatcttc aaggcgagga cgggtatcgc
     2401 cgtcaaggtc gtgagccaag gcacggggca ggccatcgat acgggcaagc gcggcgatgc
     2461 cgacgtcgtg ttcgtgcacg ccaagccgca ggaggggaag ttcgtcgccg acggcttcgg
     2521 ggtgaagcgc tacgacgtga tgtacaatga cttcgtgctc atcggtccga agggcgatcc
     2581 ggcggcaatc aagggtggcc gcgacatcct cgcggcgctc acgaccatcc atgcgaaggg
     2641 cgccccgttc gtctcgcgcg gcgacaagag cggcacacac gcagccgaac tcgcgctctg
     2701 gaaggcagcg agcctcgatc ccgcggcgtc gaagtccgca tggtatcgcg agattgggca
     2761 aggcatgggc gcggcgctca atacggccgc ggcgatgggc gcttacgttc tcgccgatcg
     2821 cggcacgtgg ctctccttca agaaccgcga ggcgctcctg atcgccgtcg agggcgaccg
     2881 tcggctcttc aatcagtacg gcatcatcct cgtcaatcca gcgaagcata agcacgtgaa
     2941 gcgcgaactc gggcagtcgt ttgtcgactg gatcgtctcg aaggaggggc aggacgccat
     3001 tgccggcttc aagatcaacg gcgagcaact gttcttcccg aacgcgggca gtgcgaaata
     3061 ggctatcact cgtctccggc aaccgtgcgg aggcagcagc atgattctga tcgggcaata
     3121 cgattccccc ttcgtgcgcc gcgtcggcat tgcgatgcgg ctctatggca tcaccttcga
     3181 gcaccggcca tggtcggtgt ttgcggatgc tgaaaagctg cggctgctca atccgctgac
     3241 gcgcgtgccg accgtcgtcc tggatggcgg cgatgtgctc gtcgagagcc acgccatcct
     3301 cgaccatctg gatactctcg tcgtcccgga acagcgcctc ttccccgtcg cggagccgga
     3361 gcggcatcgc gcgattgcga tcgcggcgct ggcgacgggt ctcgccgaca aggccgtgag
     3421 cctattctac gagacgcggc tgcaccggga ggtgtcggag gtctggacag ggcgctgccg
     3481 ggcgcagatc ggcggtgtga tgcgggagct cgagagccgt tatgccggtt gcccgggacc
     3541 ctggtggcag ggagagcgca tcgggcatgc cgnnnnnnnn nnnnnnnnnn nncgggcgag
     3601 gcgcatccgg gcctcatcga tctcgacgcg gcgccgcggc ttgcggtgct gacggtgcag
     3661 gccgaggcgc tccccgcttt cagcgagatt tcacagcctt ttctgccgcc gacttgacgg
     3721 caaggttcga ctcgcgcgaa ccggcaggat gcgtcctcac ttccgtcatg gtttcggtcg
     3781 agaagggcgg cccgtgcact gagggacggc ctgcgatgac gagacggcag accactgcgc
     3841 cgacgcgacg cgacgtcttc aggctcgcgg tgggggcgtc tgcactcagt gccggcgtcg
     3901 cggcggttgg cgaggacgtc cgcgcgcagg gcaaaggccc gatccgtgtc gacgtcgaca
     3961 gctcgagcct caggccgcgg ccgatcgcga tcccgatttt tctgtccgag gattcgaagc
     4021 tcggcgccga ggtcgccgac atcatcgccg cggatctcgc cggctccggg ctgttccagc
     4081 cgctcgatcg gtcggcgttt ccgcccgtgc gcgatttcaa tgggccgatc cagttcgccg
     4141 attggcgggc cgggggggca gaggcgctgc tgacgggacg tgtcagtcga ttgcctgacg
     4201 ggcggatcga cggcgctttc cgcttgtggg acgtggtgct cgagaaggcg ctcgacgggc
     4261 agcgtgtggg aaccacgccg cagtcctggc ggcgattcgc gcacatcatt gccgatcgtg
     4321 tctacgagaa gatgacgctc gagcgcggtt acttcgatac gcgcgtggtc tttgtcgacg
     4381 aaaccggcgc gaaggaccgg cgtgtcaagc gtctcgcgat catggatcag gacggccaca
     4441 acgtcgggtt gctgtcggat ggacgggagc tggtgctgac gccgcgcttc agcccgacgg
     4501 ggcagatgat cgccttcatg tcgtatgcgg ggcagaagcc gagtgtcggg accatgaacc
     4561 tcgcgacgcg cgagcggcaa ctcatcggcg acctgggcgg catgacgttt gcgccacgct
     4621 tctcacccga cggccaacgc ctcgtcttca gcctgcagag cgaaggcagc tcgagcatcg
     4681 tcgaaatgga cctgcggtcg cgccagtcgc ggcggctcac ccagggcccg gcaatcgaca
     4741 cggcgccctg cttctcgccc gacggccagt cgctcgtgtt cgaatcggat cgcgagggga
     4801 atcagcagct ctacgtgatg gctgcgggcg gcggtccggc gcggcgcctc agttacggcg
     4861 aggggcgcta ctcgacgccg gtctggtcgc cgcggggcga caccatcgcc ttcacgaagc
     4921 aggcgagcgg ccgcttcctg atcggcgtga tgcggacgga tggctcgggc gagcggattc
     4981 tcacagacgg cttccacaac gaggggccga catgggcgcc gaatggccgt gtcctgatgt
     5041 tcttccgcga ggagcggggg gcgaatgccg gcccgcgcat cttctcggtc gacctcaccg
     5101 ggcgaattga gcgtgcgatt ccaacgccat ccttcggctc cgacccggcc tggtctccgt
     5161 tgctgaaata ggacggccgc taactggtcg tttaccaatc cagcgttagt ttgcgacaca
     5221 agtcgtgtgc cgagaaccgg catctgtgct gtgctcttgg tgcaattcgt ccgtcggtcc
     5281 agtcatgatc gatgggctcg atgagctatg gtcagcatga gcaagacggg ttgagcaagc
     5341 cgttgcattt tcgcatcaaa caggggcgaa acgacgtgcc gggaagcctc agttcttgat
     5401 ctcgtgcctg cccgcaagta cccctaatcg tgcgattgcc gttccagaag caggagagaa
     5461 gcatgaaggt caagacaatg gtcgtcctcg cgacggtcat cgcagcggtg gcggtcagcg
     5521 cttgccgccg cgaagttgcg cacaagccg
//
LOCUS       DAORNH010000306         5530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10894_length_5530_cov_2.420639, whole genome shotgun sequence.
ACCESSION   DAORNH010000306 DAORNH010000000
VERSION     DAORNH010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_5530_cov_2.420639"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PK264_21780"
     CDS             complement(<1..1372)
                     /locus_tag="PK264_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRD78534.1"
                     /translation="MARVFDPRASRATLFSALLAAKRQHGGDKAIIEDVERQPLTYSR
                     IILGSFVLGGRLAAMTRRKEAVGVLLPNVAGLAVVLFGLNAYGRVAAMLNFTAGVRNL
                     RSALRTAEVRTIVTSRRFIATAKLEDVVAALAETETAKGGKVRIVYLEDVRKEIGAKD
                     KIGGVIRAAMAARVHRKHGLGPDDPAVILFTSGTEGEPKGVVLSSANLVANAAQIYAH
                     ADGALSPADVIFNPLPMFHSFGLTAGSLVGLLNGIKVVLYPSPLHFHQIPELIAATKA
                     TFLFATDTFAQSYARAAGEGELASVRYVIAGAEKVKDQTRSQWAKFGALVLEGYGATE
                     CSPVIACSLPDANCAGSVGRALPGMELRLEAVEGITDGGRLYVRGPNVMSGYMMSKYP
                     GVLVPPEGGWHDTGDIVAIDADGFLTIKGRAKRFAKVGGEMISLAAVEGVVASLWPEA
                     SHVVVSL"
     gene            1658..2968
                     /locus_tag="PK264_21785"
     CDS             1658..2968
                     /locus_tag="PK264_21785"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HRD78535.1"
                     /translation="MRYLMATVSAVALVAVSSGTASAGGFAVREQSVYHQGASFAGSA
                     AGGALSSMFWNSAAAAFAPGGIYMESNYSAILGNVEITAEPGSTLFGALPSDSGNIAK
                     PAVVPASYTSMRLSENVVFALGINSPFGLVTEPSNRFWAGQTHTRTSEIKTYNFNPTL
                     AYRFSPTLAVGVGLQIEHIEGRLKSASGVLATSRNVVIKGDDTAFGFTLGVMYTPTAN
                     TTVGLGWRSSIDHTLEGTTSIPGSPVPPADAGVAIKAGTTLPDIVTLSIRHALSSHWT
                     LLGTVEWTRWSNVDGLTVRCANTTPNAVFCPAGNGQQVRRLELGWDDGWFFALGAEYT
                     ANERMKVRAGVAYEMSPITDPEGRSLRVPDTDRLWLSVGATYKWSEMMTFDAAYTHIF
                     GIGDDKIDRTESGIRYVGNVDASVDILSVAARIKLGHPAPSPMK"
     gene            3084..>3399
                     /locus_tag="PK264_21790"
     CDS             3084..>3399
                     /locus_tag="PK264_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRD78536.1"
                     /translation="MSVRNGREFLSIPGPTNVPDEVLQAMHRPAVDIYSGGMVALGDA
                     IHMDMRKVFGTKERVYIYSANGHGGWEAAFSNVLSKGDLVLVLESGRFAIGWGEMAEM
                     MGA"
     assembly_gap    3400..3453
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3454..4268
                     /locus_tag="PK264_21795"
     CDS             <3454..4268
                     /locus_tag="PK264_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRD78537.1"
                     /translation="RLKKDKTHEIKAIMVVQIDTASSCVNDIPAIRKAIDAAKHPALL
                     MVDCVASLGCMPFEMDAWGVDVAMAGSQKGLMTPPGLAFVTASQKAREIHKSAGMRTM
                     YWDWTFRDGPISYHKYCGTPPEHLLFALRKSIDLLFAEGMPNAFRRHALLAEATRRAV
                     ERWSEGQALGFCVEVPAERSNSVTNVLAKSFDPQTLLDYCRETCGVVLGVGLGDLSGK
                     AFRIAHMGHTNAPMVLGTLGAIEMGLKALSIPHGRGGSEAAIEYLAANAKAH"
     gene            complement(4487..5026)
                     /locus_tag="PK264_21800"
     CDS             complement(4487..5026)
                     /locus_tag="PK264_21800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD78538.1"
                     /translation="MVDRPRVPVRLRAARLSELPALAKMRIALQEHLKASNPHVWMLD
                     RHSLPAIEANYRDMLTKGGDRICFLVAEAAGSTQPRAMAIGRVAERPEFTPAVTGLID
                     DVWVDPELRGGGIAKALLADLAAFFRRHNAETIVLDYVIGNREAERLWGSLGFTPVQV
                     GADARLGDFERKLRGDPHR"
     gene            5209..>5530
                     /locus_tag="PK264_21805"
     CDS             5209..>5530
                     /locus_tag="PK264_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HRD78539.1"
                     /translation="MTGLNQIRSTFLDFFKKNGHEVVASSSLVPRNDPTLMFTSAGMV
                     QFKNTFTGQESRPYSRAVTAQKCLRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KERAI"
BASE COUNT          976 a         1870 c         1704 g          926 t
ORIGIN      
        1 gcaggctcac aaccacatgg cttgcctccg gccagagcga cgccacgacg ccctcgaccg
       61 cggcgagcga aatcatctcg ccgccgacct tggcgaagcg cttcgcgcgc cccttgatgg
      121 tgaggaaacc gtccgcgtcg atggcgacga tgtcaccggt gtcgtgccag ccgccctcgg
      181 gcggcacgag aacgcccgga tacttcgaca tcatgtagcc cgacatcacg ttcgggccgc
      241 gcacgtagag ccgcccaccg tccgtgatgc cctcgaccgc ctcgaggcgc aactccatgc
      301 cggggagcgc gcggccgacg gagcccgcac agttggcatc gggcaggctg caggcgatca
      361 ccggcgagca ctcggtggcg ccgtagcctt cgagcacgag cgcgccgaac ttcgcccact
      421 gggacctggt ctgatccttc accttctcgg ccccggcgat gacgtagcgg acgctcgcaa
      481 gttccccctc gcctgccgcc cgcgcgtagc tctgtgcaaa cgtgtcggtc gcgaacagga
      541 acgtcgcctt cgtcgccgcg atcagctccg ggatctggtg gaaatgcagg gggctcgggt
      601 agaggacgac cttgatacca ttaaggaggc ccaccagcga acccgccgtc agtccaaacg
      661 agtggaacat cggcagcggg ttgaagatga catcggccgg tgacagcgcc ccgtcggcat
      721 gcgcataaat ctgcgccgca ttggcgacga ggttggcgct cgaaagcacg acacccttgg
      781 gctcaccttc ggtgccggac gtgaagagaa tgaccgccgg atcatcgggt ccgaggccgt
      841 gcttcctgtg cacgcgcgcc gccatggccg cgcggatgac gccgccgatc ttgtccttgg
      901 cgccgatctc cttgcgcacg tcctcaagat agacgatccg gaccttgccg ccctttgccg
      961 tctctgtttc tgcaagtgcg gcgacgacgt cctcgagctt ggccgtcgcg atgaagcgcc
     1021 gcgaggtgac gatcgtgcgc acctcggccg tgcgcagcgc cgagcgcaga ttgcgcacgc
     1081 cagccgtgaa attcagcatc gccgcgacgc gcccataggc attgagaccg aacagcacga
     1141 ccgcgagacc ggcgacgttc gggaggagta cgcccacggc ctccttccgc cgcgtcattg
     1201 ccgcgagccg cccgccgagc acgaagctgc cgaggatgat ccgactgtag gtgagcggct
     1261 ggcgctcgac atcctcgatg atcgccttgt cgccaccatg ctgtcgcttg gcggccagca
     1321 gtgccgagaa cagtgtcgct cgtgacgcgc ggggatcgaa gacacgggcc atgcggaatc
     1381 tcgcctcgat tgaccgagcg agagtggccc cgccgcggat caactgcaag tccggcgacg
     1441 ctccggcggg tcgatgccgg cgcaccgctg cccactcttg tggcaacgga ggcacacgtt
     1501 gcctggattc aacccaaaca gcaccactgc gcacctgcag cccgttgcag cggcctcaag
     1561 cccctgctta aggtgtcaac aatgtggcag ggggaatcca tcgccacacg gaaatgcttc
     1621 ggacggatct tcgggacacc cacgacgggg gacgctaatg cgctacttga tggcaactgt
     1681 cagtgctgta gctttggtgg cagtttcgag tggcacggcg agcgcgggtg gtttcgcggt
     1741 tcgtgagcag tcggtttatc accagggtgc ctcgtttgcc ggcagcgctg ccggcggcgc
     1801 gctctcatcc atgttctgga actcggcagc tgcggccttc gcacccggcg gcatctacat
     1861 ggagagcaac tactcggcca tcctcggtaa cgtcgaaatc acggcagaac cgggttcgac
     1921 acttttcggg gcgctgccat ccgatagcgg gaacatcgcc aagccagcag tggtgccggc
     1981 ctcatacacc agcatgcgcc tcagcgagaa cgtcgtcttc gcgctcggca tcaactcgcc
     2041 attcggcctg gtgaccgagc cgagcaaccg cttctgggcc ggccaaacgc acacgcgcac
     2101 ttcagagatc aaaacctaca atttcaatcc gacgctcgcc taccgcttca gcccgacgct
     2161 cgccgtcggc gtcggtttgc agatcgagca catcgaaggc cgactcaaaa gtgcgagcgg
     2221 cgtgctcgcg acgtcgcgca atgtcgtcat caagggagac gacacggcat tcgggttcac
     2281 actcggcgtc atgtacacgc cgacggccaa caccacggta gggttgggct ggcgctcctc
     2341 tattgaccac accctcgaag gcacgacgag cataccagga agccccgtac ccccggcgga
     2401 tgccggtgtc gccatcaagg ccggcaccac gctaccagat atcgtcacgc tcagcattcg
     2461 tcacgcgctc tctagccact ggacgctgct cggcacggtc gaatggactc gatggagcaa
     2521 cgtggacggc ctcaccgtca ggtgcgccaa caccacgccg aacgctgtct tctgccctgc
     2581 cggtaatgga cagcaggtgc gccgtctcga actcggctgg gacgatggct ggttcttcgc
     2641 cctcggcgcc gagtacacgg cgaacgagcg catgaaagtc cgcgcgggcg tcgcctacga
     2701 gatgtcgcca atcaccgatc ccgagggtcg ctcgctgcgc gtgcccgaca ccgatcgact
     2761 ctggctcagc gtcggcgcca cctacaagtg gagcgagatg atgaccttcg acgccgccta
     2821 cacccacatc ttcggcatcg gcgacgacaa gatcgaccgc accgagagcg gcatccgcta
     2881 tgtcggcaac gtcgatgcca gcgtcgacat cctctcggtc gctgcacgca tcaagctcgg
     2941 ccacccggct ccttctccga tgaagtgatc taggccacag tttcatcatg acgcgggcgc
     3001 aaccccggtt gcgcccgttg tcatttgcgc cgccgcgtgt cacggtcgcg ccaatcccga
     3061 tccactcatg cgcgaggcgc accatgtccg ttcgcaacgg tcgcgaattc ctgtccatcc
     3121 cgggtccgac caacgtgccc gacgaggtgc tgcaggcgat gcaccgcccc gccgtcgaca
     3181 tctactccgg cggcatggtg gctctcggcg acgcgatcca catggacatg cgcaaggtgt
     3241 tcggcacaaa ggagcgcgtc tacatctact ccgccaatgg ccacggcggc tgggaagccg
     3301 ccttctcgaa tgtcctctcg aagggcgatc tcgtgctcgt cctcgagagc ggccgcttcg
     3361 ccatcggctg gggcgagatg gccgagatga tgggtgcccn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcgtct caagaaggac aagacgcacg
     3481 agatcaaggc catcatggtc gtccagatcg acaccgcgtc gagctgcgtc aacgacattc
     3541 cggccatacg caaggccatc gacgccgcca agcacccggc gctcctgatg gtcgattgcg
     3601 tcgcctcgct cggctgcatg cctttcgaga tggacgcctg gggcgtcgat gtcgccatgg
     3661 cggggtccca gaagggcttg atgacgccgc ctggcctcgc cttcgtgacc gcgagccaga
     3721 aggcccgcga gatccacaag tccgcaggca tgcgcacaat gtactgggac tggaccttcc
     3781 gtgatgggcc gatcagctat cacaagtact gcggcacgcc gccggagcat ctcctgttcg
     3841 cgctccgcaa atcgatcgac ctcctgttcg ccgagggaat gcctaatgcc ttccgccgcc
     3901 atgccctcct cgccgaggcg acacggcgcg cggtcgagcg ctggtcggag ggtcaggcgc
     3961 tcggcttctg cgtcgaggtg ccggcagaac gctcgaactc ggtgaccaat gtgctcgcca
     4021 agagcttcga tccgcagaca ctcctcgact attgccgcga gacctgcggc gtcgtgctcg
     4081 gcgtcggcct cggcgacctc tcggggaagg cgttccgcat cgcgcacatg ggccacacca
     4141 atgcgccgat ggtgctcggc acgctcggcg ctatcgagat gggcctcaag gcactctcga
     4201 ttccgcacgg ccgcggcggc agcgaggctg cgatcgaata tctcgccgcc aacgcaaagg
     4261 cgcactaggg ggagcgctgg gcctgcccca gtttcgtgga gcgtattgat ttggggatca
     4321 tgcgcggctg attgttgcgg aggccaatgc ccccttcttt gagggactaa tcttatattc
     4381 tggcccctgc ggagcccttc cttccgtgcc ggccggagag cttcgcgcag caggcgcgcc
     4441 tccggcacga cgcgccagtc ccgcacgcgc ggcgccggac gagccgtcat cggtgcgggt
     4501 cacctctgag cttccgctcg aaatcgccga gccttgcgtc ggcgccgacc tggacaggcg
     4561 tgaagccgag gctgccccag agccgctcgg cctcacgatt accgatgacg tagtcgagga
     4621 cgatcgtctc ggcgttgtgg cggcggaaga aggcggcgag gtcggcgagg agggccttgg
     4681 cgatgcctcc gccacggagc tccggatcga cccagacgtc atcgatgagg cccgtgacgg
     4741 cgggcgtgaa ctcgggccgc tcggcgacgc gaccgatggc catggcgcgc ggctgcgtgc
     4801 tgccggcagc ctcggcgacc agaaagcaga tgcggtcgcc gcctttcgtc agcatgtcgc
     4861 gatagttggc ctcgatcgcg gggagtgagt gccggtcgag catccagacg tgcgggttcg
     4921 atgccttgag gtgctcctgg agggcgatgc gcattttggc gagggccggc aactcgctga
     4981 gacgtgcagc cctgagcctg accgggacac gagggcgatc aaccatttcc agtccctcct
     5041 caattctcgt tcatgctcgc ggacggagcg aaaccggcaa cttgcgacgt gcgcgcccgt
     5101 atcctcggcc cgcgcctcag gtggacagcc tccggggagg cgtctaaaac cgcgaacctg
     5161 ggtcgcctca ggcccaccat cttccgaaca tcctgacaag gcgagaccat gaccggcctc
     5221 aaccagatcc gatcgacctt cctcgacttc ttcaagaaga acggtcacga ggtcgtcgca
     5281 tcttcctcgc tcgtcccgcg caacgatccg acgttgatgt tcacctccgc cggcatggtg
     5341 cagttcaaga acaccttcac cggtcaggaa agccgcccct attcgcgcgc cgtaacggca
     5401 cagaagtgcc tccgcgccgg cggcaagcac aacgacctcg acaatgtcgg ctacaccgcg
     5461 cgccaccaca cctttttcga gatgctcggc aacttctcgt tcggcgacta cttcaaggag
     5521 cgcgcgatcg
//
LOCUS       DAORNH010000307         5526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10910_length_5526_cov_2.518187, whole genome shotgun sequence.
ACCESSION   DAORNH010000307 DAORNH010000000
VERSION     DAORNH010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10910_length_5526_cov_2.518187"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1216)
                     /gene="glcF"
                     /locus_tag="PK264_21810"
     CDS             complement(<1..>1216)
                     /gene="glcF"
                     /locus_tag="PK264_21810"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HRD78540.1"
                     /translation="CVHRGLCTATCSTYVLLGDERDSPRGRIYLMKDMFEKGAAASGE
                     VMHHVDRCLSCLSCMTTCPGGVDYMHLVDHARAHIEATGQRTRKQRLLRTLLANLVPY
                     PDRFRRALRLAPLGRPFRSLMTRFGFPEFVAMIDLAPRLGRRRARFSGAGTAETKTER
                     QKRVLLLPGCAQQVIRPEINDSTIRVLARRGVDVVVAPNTGCCGALVHHMGKEALAIA
                     QAKANVDAWTAAMAKGPIDAIIINASGCGTTVKDYGHMLAREEGYAEKAKTVSALTRD
                     VTEFLSSYDLGPPRRWSSLRVAYHSACSMQHGQRITDEPKALLRKAGFTVLDVPEAHI
                     CCGSAGVYNILQPELAGELRKRKVGHIESVRPDLIAAGNLGCITQIGLGTQVPIVHTV
                     ELLDWAYGGPVPT"
     assembly_gap    1217..1251
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1298..2506)
                     /locus_tag="PK264_21815"
     CDS             complement(1298..2506)
                     /locus_tag="PK264_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD78541.1"
                     /translation="MRPATERELAEMLAAASAERTPLEIVGNGSKTSIGRPVQAHARL
                     STASLKGVTLYEPTELVMSALAGTPLSRIEAELAANGQMLAFEPLELGPLCGGPPGQA
                     SIGGVFMSNLSGARRIVSGAARDHLLGVRALGGAGQTIKSGGRVMKNVTGYDLTKLLA
                     GSWGTLAVATELTFKVLPAPEATATLMLLGLSDDLAIEALCHAMGTPYEVTGAAHLQA
                     PVAQRLTTESVRAAGRAATLIRLEGFTKSVAYRGGHIRKELAAFGEVHVLSTDASLEL
                     WSELQRLSAVAEQTDPLWRISTSPASGAKVVAAIRQYMDCRVLYDWSGGLIWLEVLAT
                     ADAGAADIRRVVASHGGHATLVRAAPAVRASVDVFQPMEQGVADLTRGIKSVFDPAQI
                     LNPGRMYAGV"
     gene            complement(2618..4045)
                     /locus_tag="PK264_21820"
     CDS             complement(2618..4045)
                     /locus_tag="PK264_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRD78542.1"
                     /translation="MSMIAMPEPDRALVQRRDQIARDLRLLVPKATVISDADGRRAYE
                     TDGLTAYRAMPLAVVLPRSTDDVAAVLRYCHTHRIKVVARGAGTSLCGGALPTEDAIV
                     LCVSKMNRVLDVDFQSRTAHLEAGITNLGITAAVSGKGFFYAPDPSSQLACTLAGNLA
                     MNSGGAHCLKYGVTTNNVLGLKMVMMDGRVVEIGGAHLDAGGYDLLGLIVGSEGQFGI
                     ITEATVRILRSAEGARPMLLGFKSSEIAGACVSAIIASGIIPVAIEFMDKPAIEVCEA
                     FAKAGYPLDVEALLIVEVEGSTEEIADLIGRIVEIANRFEPKVVRVSQSSEQSAAIWK
                     GRKAAFGAIGRISDYYCMDGTIPLKRLPEVLTKIDEICKRQGLRVANIFHAGDGNLHP
                     LILYDANEPEEMERAEKAGAEILTLCVEVGGCLTGEHGVGIEKRDLMRVQFSETDLAL
                     QMRIKTVFDPAWLLNPAKVFPLDAR"
     gene            4252..>4379
                     /locus_tag="PK264_21825"
     CDS             4252..>4379
                     /locus_tag="PK264_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD78543.1"
                     /translation="MTAITDLEIFARVARTGNMSAAGREMGLSPAVVSKRISLLEER"
     assembly_gap    4380..4389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4390..5269
                     /locus_tag="PK264_21830"
     CDS             <4390..5269
                     /locus_tag="PK264_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD78544.1"
                     /translation="RLGARLFQRTTRQLTLTETGEGYFKRVVDILSLIAEAEDFVSRR
                     NTKPRGLLKVTAPISFSRLHVAPYLPEFLSRYPEIELDFQLTDNFVDIIRDGFDLAIR
                     IGELQDSSLVQRKLTSDTRVLCASPKYLEQHGVPKSLADLEMHNCLSAGAQDSTWRLE
                     GPGGAQSVRVKGNIRSNSFEFMREALLAGLGLGLRSTWDIGAELKSGDIKIVLPEYRG
                     SSNVAIYAVYPCRDFMPAKVNVFIEYLAELYGNDPYWDRGLDLQRFAVGAQKPSRERD
                     STSASRTHRDAAPAGR"
BASE COUNT          937 a         1769 c         1849 g          926 t
ORIGIN      
        1 ggtcggcacc ggcccgccgt aggcccaatc cagcagctcg accgtatgca cgatcggcac
       61 ttgcgtgccg agcccgatct gcgtgatgca gccgagattg ccggccgcga tcaggtcggg
      121 acggacgctc tcgatatgcc cgaccttgcg cttgcgcagc tcgcccgcga gctcgggctg
      181 gaggatgttg tagacgccgg ccgagccgca gcagatgtgg gcctccggca cgtcgaggac
      241 cgtgaacccg gccttgcgca gcagcgcctt cggttcgtcg gtgatgcgct ggccgtgctg
      301 catggagcag gccgaatggt aggcgacgcg caggctcgac caacggcgcg gtggcccgag
      361 atcgtaactc gaaaggaact cggtcacgtc gcgggtaagt gccgagaccg tcttggcctt
      421 ctccgcatat ccttcctcgc gggcgagcat gtggccgtag tccttgacgg tggtgccgca
      481 gcccgacgcg ttgatgatga tggcgtcgat cggccctttc gccatggccg ccgtccaggc
      541 atcgacgttg gccttggcct gggcgatggc aagcgcctcc ttgcccatat ggtggacgag
      601 ggcgccgcaa cagcccgtgt tcggcgcgac gacgacgtcg acgccgcgcc gggcgaggac
      661 gcggatggtc gaatcgttga tctcgggccg gatcacctgc tgcgcgcatc cgggcagcag
      721 caagacgcgc ttctgccgct cggttttcgt ctcggccgtg cctgcgcccg agaatcgcgc
      781 acgccgccgg ccgagccgcg gggcgaggtc gatcatggcg acgaactcgg gaaagccgaa
      841 gcgcgtcatg aggctccgga acgggcggcc gagcggagcg agccgcagcg cgcggcgaaa
      901 gcggtccggg taaggaacga gattggcgag aagcgtgcgc aggaggcgct gcttgcgcgt
      961 gcgctggccc gttgcctcga tgtgggcgcg ggcatgatcg accaagtgca tgtagtcgac
     1021 gccgccaggg caagtggtca tgcaggagag gcaggagaga cagcggtcga cgtgatgcat
     1081 cacctcgccg ctcgcggcgg cacccttctc gaacatgtcc ttcatgaggt agatgcgccc
     1141 gcgcggggaa tcgcgctcgt cgccaagcag gacataggtc gagcaggtcg ctgtgcagag
     1201 gccacggtgc acgcagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgagctggt
     1261 ggggctggaa gttggtctgc atcgtgcctc tttgacgtca cacgccggcg tacatgcggc
     1321 cgggattgag gatttgcgcc ggatcgaaca ccgacttgat gccccgcgtc agatcggcga
     1381 caccctgctc catcggctgg aacacgtcga cggaggcccg cacggcggga gcggcccgca
     1441 cgagcgtcgc atggccgcca tggctcgcca cgacgcggcg gatgtcggcg gcaccggcgt
     1501 cggccgttgc cagcacctcg agccagatga ggccgccgga ccagtcgtag aggacgcggc
     1561 agtccatgta ctggcggatg gcggcgacga ccttggcgcc gctcgccggc gacgtcgaga
     1621 tgcgccatag cgggtcggtc tgctcggcga cggcggacaa gcgctgcagt tccgaccaca
     1681 gttcgagcga cgcgtccgtc gacaggacat gcacctcgcc gaatgcggca agctctttcc
     1741 tgatgtgacc accccggtat gcgacagact tggtgaagcc ctcgaggcgg atcagcgtcg
     1801 cggcccggcc ggcagcgcgg acactctccg tcgtcaggcg ttgcgcgacc ggggcctgca
     1861 ggtgcgccgc acccgtgacc tcgtagggcg tccccatggc gtggcagagc gcctcgatcg
     1921 cgagatcgtc ggagagcccg agcagcatca gtgtggccgt ggcctcgggt gccggcagca
     1981 ccttgaaggt aagctcggtc gcaacggcaa gcgtgcccca gctgcccgcg agcagcttgg
     2041 tgagatcata acccgtcacg ttcttcatga cgcggccgcc gcttttgatg gtctggccgg
     2101 cgccgccgag cgccctgacg ccgagcaggt gatcacgcgc ggcgcccgag acgatgcggc
     2161 gagcacccga caaattgctc atgaagacgc caccgatgct ggcctggccg ggcggaccac
     2221 cgcaaagcgg tccgagctcc agcggctcaa aggcgagcat ctgcccgttg gctgcaagct
     2281 cggcctcgat gcgcgagagc ggcgtgccgg cgagcgccga catcacgagc tccgtcggct
     2341 cgtagagggt gacacccttg agggaggcgg tcgacaggcg ggcatgcgcc tgcaccggcc
     2401 gaccgatgct cgttttcgag ccgttgccca cgatctcgag gggcgtacgt tccgcggacg
     2461 cggcggccag catctccgca agctcgcgct cggtcgccgg tctcatctgt gtctgcaagg
     2521 cttttccccg aggccgatcg caccatcggc cattcgagag cacgcgagcc gtgccatggc
     2581 aagcccatac cgcggagtgc tcggtcaacg cgcaatatca gcgcgcatcg agcgggaaaa
     2641 ccttggcggg attgagcagc caggccggat cgaagacggt cttgatgcgc atctgcaggg
     2701 cgaggtctgt ttcggagaac tggacgcgca tcagatcacg cttctcgatg ccgacgccat
     2761 gctcgccggt gaggcagccg ccgacctcga cgcagagcgt gagaatctcg gcgccggcct
     2821 tctcggcgcg ctccatctct tctggctcgt tggcgtcgta gaggatgagc ggatggaggt
     2881 tgccgtctcc ggcgtggaag atgttggcga ccctgaggcc ctggcgcttg cagatctcgt
     2941 cgatcttggt cagtacctcg ggaaggcgct tcagggggat ggtgccgtcc atgcaatagt
     3001 agtcggagat gcgtccgatc gctccgaaag cggccttgcg ccctttccag atcgcagcac
     3061 tctgctccga gctctgactc acgcgtacga ccttgggctc gaaccggttg gcgatctcga
     3121 cgatgcggcc gatgaggtcg gcgatctcct cggtcgaacc ctcgacctcg acaatcagca
     3181 gcgcctcgac gtcgagcgga tagccggcct tggcaaaggc ctcacagacc tcgatcgcag
     3241 gcttgtccat gaactcgata gcgaccggga tgatgccgct cgcgatgatc gccgacacgc
     3301 aggcgccggc gatttcgctg gacttgaagc cgagcagcat cggccgtgcg ccctcggctg
     3361 atcggaggat gcggacggtc gcctcggtga tgatgccgaa ctgcccttcc gagccgacaa
     3421 tgagcccgag gaggtcgtag ccgccagcgt cgaggtgcgc gccaccgatc tcgacgacgc
     3481 ggccgtccat catcaccatt ttgaggccga gcacgttgtt ggtcgtcacg ccgtacttga
     3541 ggcagtgcgc gccgccggag ttcatagcga gattgccggc gagcgtgcag gccagctggc
     3601 tcgaaggatc gggcgcgtag aagaagccct tgcctgagac agcggcggtg atgccgagat
     3661 tcgtgatgcc ggcctcgagg tgcgccgtcc ggctctggaa atcgacatcg agaacgcggt
     3721 tcatttttga cacgcacagg acgatcgcgt cttccgtcgg cagtgcgccg ccacacagtg
     3781 acgtgcctgc gcctcgcgcc accaccttga tgcgatgggt gtggcaatag cggagtacgg
     3841 ccgcgacatc gtcggtggag cggggcagga cgacggccag cggcatcgcc cggtaggccg
     3901 tgaggccatc tgtttcgtag gcgcgccgcc cgtcggcgtc ggagatgacg gtcgccttgg
     3961 gcaccagtag gcgaagatcg cgcgcgatct ggtcgcgccg ctgcaccagt gctcgatccg
     4021 gctcgggcat ggcgatcatg gacatggtga cgatctcctg gcgaatccta aggtcagccc
     4081 ctgatcgtat tcattacgaa ggcggacccg agcattattt tcaacgcagg gacaaaaatc
     4141 aatgctatgg gacctaatgc tggagacaaa cattagtcac ggggcagcat aatctacgaa
     4201 cgggagaaag ttctcatgcg tttcagacgc gcaagccgag aggaaagtgc cgtgaccgcc
     4261 atcaccgatc tcgagatttt tgcacgcgtt gcgcggaccg gcaatatgtc cgcggcaggc
     4321 cgcgagatgg gtctctcgcc ggcggtcgtc tcgaagcgca tcagcctgct tgaggaacgn
     4381 nnnnnnnnnc cggctcggcg cccggctgtt ccagcgcacc acccgccagc tcaccctgac
     4441 ggaaaccggc gaaggctact tcaagcgcgt cgtcgacatc ctctcgctga tcgccgaggc
     4501 ggaggacttc gtctcgcggc gcaacacaaa gccccgcggt ctcctcaagg tgacggcgcc
     4561 gatctcgttc agccggttgc atgtcgcccc ctacctcccc gagttcctct cgcgctaccc
     4621 cgagatcgag ctcgacttcc aactcacgga caacttcgtc gacatcatcc gcgacggctt
     4681 cgatctcgcc atccgcatcg gcgaattgca ggattcctcg ctcgtccagc gcaagctcac
     4741 ttctgatacg cgcgtcctct gcgcgagccc gaagtatctc gagcagcatg gcgtgccgaa
     4801 gtcgcttgcc gacctcgaga tgcacaactg cctctctgcc ggcgcgcagg acagcacctg
     4861 gcgcctcgaa ggaccgggcg gcgcgcagtc ggtacgcgtg aagggaaaca tccgctccaa
     4921 ctcattcgag ttcatgcgcg aggcactgct tgccgggctc ggcctcgggc tgcgctccac
     4981 ttgggacatc ggcgccgagc tgaagagcgg cgacatcaag atcgtgctgc ccgagtaccg
     5041 cggctcctcc aacgtcgcaa tctacgcggt ctatccgtgc cgtgatttca tgccggcgaa
     5101 ggtcaacgtg ttcatcgagt acctcgccga gctctatggc aatgatccct attgggatcg
     5161 cggcctcgac ctgcagcgct ttgccgtcgg cgcccagaag ccgagccgcg agcgcgacag
     5221 cacgagcgcg agccgcaccc atcgcgacgc ggcgccagcc ggtcgctgaa gggggtcgct
     5281 gaagggtatc gctgaagtgt cgggtcggtt gcagcgattc gacgcgttcg cgcgccggct
     5341 cctttgcctt agatcatcat ggcgagccga ggctatgccc tcggctgtgc ctagagccgc
     5401 gcccgaggcg gacgcgagga aaagattggg gtgagcttga aatgatcaga tcggctgtct
     5461 tggcaacgct gctgctcgtc ggcgccaccg gtgtcgtgct ggccgctgac ggcgaccctg
     5521 cggaag
//
LOCUS       DAORNH010000308         5511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_10949_length_5511_cov_2.492485, whole genome shotgun sequence.
ACCESSION   DAORNH010000308 DAORNH010000000
VERSION     DAORNH010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10949_length_5511_cov_2.492485"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PK264_21835"
     CDS             complement(<1..1233)
                     /locus_tag="PK264_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HRD78545.1"
                     /translation="MLVNTERFKGNASAALKDAQLQRALAGLPTGLVARRTQARAGLP
                     EFEALRDVAKGIKEHTLENLDLYLEAWEKKATAAGAKVHWAANAAEAREIILGICRSV
                     DAKVVTKGKSMISEEIGLNAHLEATGIEVVETDLGEYLVQIRKETPSHIIAPAIHLTQ
                     DQVEADFRRLHTHLPKDRVLVEPHQLVGEAREVLRQKYLQAQVGITGANFLVAETGSS
                     VIVTNEGNGDLTQSLAKVHVAIASIEKIVPTLEDVSTIVRLLARSATGQEISTYTTFS
                     TGPRRPGDPDGPEQYHVVVLDNGRTEMLASPMREALRCIRCGACMNHCPVYGAIGGHA
                     YGWVYPGPIGSVLTPALIGVKTAGNLPNASTFCGRCESVCPMKIPLPKLMRDWREAEF
                     AQGLTSGTARTGLKLWSFL"
     gene            complement(1267..2058)
                     /locus_tag="PK264_21840"
     CDS             complement(1267..2058)
                     /locus_tag="PK264_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HRD78546.1"
                     /translation="MTAATRPSERRPSVGLFVTCLVDVVRPSVGFAAVKLIEAAGCDV
                     HVPVAQTCCGQPGYNSGDRETAKALARQTIAAFEPFDYVVAPSGSCGGMLKSHYPKLL
                     ADDATWAEKAKTFAAKSHELVSFLVDVRGMTSVAARFEGRATYHDSCSGLRELGIKEQ
                     PRRLLKTVAGLELAEMADCEVCCGFGGTFATKYSDISNAMVEKKCANIVGSKAEMVIA
                     GDLGCLMNMAGKLTRDGRPIEARHVAEVLAGDSATPAIGRAAKVT"
     gene            complement(2165..3415)
                     /locus_tag="PK264_21845"
     CDS             complement(2165..3415)
                     /locus_tag="PK264_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRD78547.1"
                     /translation="MAELQKGLQGLQAGADVLFLLLGAILVFAMHGGFAFLEVGTVRR
                     KNQVNALCKIIVDFALSTLAYFFIGYWIAYGILFFASAEAISGGAAGYDAQGLSLVKF
                     FFLVTFAAAIPAIISGGIAERARFMPQCLATALIVGLGYPLFEGIVWNKNFGLQDKLF
                     AGMLGAEFKDFAGSIVVHAFGGWAGLAAIRRLGPRLGRYDKGRSFGIPPSSIPWLAMG
                     SWMLCVGWFGFNVMSAQSLKSVSGLVAMNSLLAMCGGIIVAMVVGDNDPGFVHNGALA
                     GLVAVCAGSDVMHPIGALIVGGVAGGIFVVMFQKATNEWQIDDVLGVWPLHGLCGLWG
                     GIACGIFGLKALGGMGGVTFQAQLVGSLMGAAAGFIAGWIIYGIVDALFGLRLSVEEE
                     REGADLAVHKISSTPEDDIRMGRV"
     gene            3784..4200
                     /gene="sdhC"
                     /locus_tag="PK264_21850"
     CDS             3784..4200
                     /gene="sdhC"
                     /locus_tag="PK264_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HRD78548.1"
                     /translation="MSNATPQRRPVERPISPHLQVYRLQIHMLMSGLHRVTGAALYVG
                     TALLAWWLIAVASGPRYFEYVNGILGHPIGKLILFGYTWALVHHMVGGVRHLIWDTGR
                     GFDLGTVKTMSWLTIISSVVLTLLLWGVGLFLRGAF"
     gene            4204..4584
                     /gene="sdhD"
                     /locus_tag="PK264_21855"
     CDS             4204..4584
                     /gene="sdhD"
                     /locus_tag="PK264_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HRD78549.1"
                     /translation="MSMETPLSKVRGLGSAREGTMHFWRQRVTAVANVILVTYFLLVL
                     LKYAGSDYATMRAALRNPLVAIPLLLLVLSGIVHARLGMQTIIEDYVHGEGRKILMLM
                     ASTFFSALVGLTAVYAIVKIAIGA"
     gene            4657..>5511
                     /locus_tag="PK264_21860"
     CDS             4657..>5511
                     /locus_tag="PK264_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRD78550.1"
                     /translation="MAKNGTANGVASPAVNGRAYPITEHTYDVVVVGAGGAGLRATLG
                     CAEAGLKTACITKVFPTRSHTVAAQGGVAAALGNMGRDDWRWHMYDTVKGADWLGDQD
                     AIEYLCRNAPEAVYEMEHYGVPFSRTEDGRIYQRPFGGMTTEFGEGPPAQRTCAAADR
                     TGHAMLHTLYGQSLRHAAEFFVEYFAIDLIMDAEGACHGVVALCLEDGTLHAFRAHKT
                     VLATGGYGRAYFSCTSAHTCTGDGNAMVARAGLALQDHEFIQFHPTGIYGSGCLITEG
                     ARGEGGYLT"
BASE COUNT          949 a         1866 c         1748 g          948 t
ORIGIN      
        1 gaggaagctc cagagcttga gcccggtccg cgcggttccc gaggtaaggc cttgcgcgaa
       61 ctcggcctct cgccagtcgc gcatcagctt cggcagcggg atcttcatcg ggcagacact
      121 ctcgcagcgc ccgcagaacg tcgaggcatt ggggaggttg ccggccgtct tcacgccgat
      181 cagagcgggc gtcagcaccg agccgatcgg gcccggatag acccagccgt aggcgtgccc
      241 gccgatcgcg ccatagaccg ggcaatggtt catgcaggcg ccgcagcgga tgcagcgcaa
      301 ggcctcgcgc atgggcgagg cgagcatttc cgtgcgcccg ttgtcgagga cgacgacgtg
      361 atactgctcc ggcccgtccg gatcgcccgg ccggcgcggc ccggtcgaga aggtcgtgta
      421 ggtggagatc tcctgccccg tcgccgagcg agccagcagc ctgacgatcg tcgatacgtc
      481 ctcgagcgtc ggcacgatct tctcgatcga ggcgatcgcc acgtgcacct tggcgagcga
      541 ctgcgtgagg tcgccgttgc cttcgttggt gacgatcacg gacgagcctg tctcagcgac
      601 gaggaaattc gcgcccgtga tgccgacctg agcctgcagg tacttctgcc ggagcacctc
      661 gcgcgcctcg ccgacgagct gatgcggctc gaccagaacg cggtccttcg gcagatgcgt
      721 gtggaggcgg cggaaatcgg cttcgacctg gtcctgcgtc aggtggatcg ccggtgcgat
      781 gatgtggctc ggcgtctcct tgcggatctg caccaggtac tcgccgagat cggtctcgac
      841 cacctcgatg ccggtcgcct cgagatgcgc gttgagcccg atctcctccg agatcatgct
      901 cttgcccttg gtaacgacct ttgcgtcaac actccggcag atgccgagga tgatctcgcg
      961 ggcctcggcg gcattggcag cccaatgcac cttcgcgcct gccgccgtcg ccttcttctc
     1021 ccaggcctcg aggtagagat cgaggttttc gagcgtgtgc tccttgatgc ccttggcgac
     1081 atcgcgcagc gcctcgaact cggggagccc cgcccgcgcc tgggtgcgcc gcgcgacgag
     1141 ccccgtcggc agcccggcga gcgccctctg cagctgtgca tccttcagcg ccgcgctggc
     1201 gttgcccttg aatcgttcgg tattgacgag catggggtcg gtccggtcgt gcgttcggtg
     1261 atcggctcag gtgactttgg cggcccgccc gatcgcgggt gtcgcgctgt cgccggcgag
     1321 cacttcggcg acgtggcgcg cctcgatcgg gcggccgtcg cgggtgagct tgccggccat
     1381 gttcatcagg cagccgaggt cgcccgcgat caccatctcg gccttggagc caacgatgtt
     1441 ggcgcacttc ttctcgacca tggcattcga gatgtcggaa tacttggtcg cgaaagtgcc
     1501 accgaaaccg cagcagacct cgcaatcggc catctcggcg agctcgagcc ccgcgaccgt
     1561 cttgagcagg cggcgtggct gctccttgat gccgagctcg cgcagtcccg agcagctgtc
     1621 gtgataggtg gcgcgcccct cgaagcgggc agcgacgctg gtcatgcccc gaacatcgac
     1681 gaggaatgag accagctcgt gcgatttggc cgcgaacgtc ttcgccttct cggcccaggt
     1741 ggcatcgtcg gcaagcagct tcggatagtg cgacttcaac atgccgccgc aggagcccga
     1801 gggcgccacc acgtagtcaa acggttcgaa ggcggcgatc gtctgccgag ccagggcctt
     1861 tgccgtctca cggtcgcccg agttgtagcc cggttgcccg cagcaggtct gcgccaccgg
     1921 gacgtgcaca tcgcagcctg ccgcctcgat cagcttcaca gccgcaaacc cgaccgaagg
     1981 ccggaccaca tcgacgaggc aggtgacgaa caggccgacg ctcgggcgtc gctcgctcgg
     2041 acgggtagct gcagtcatgc cggattcgct cccttgccgc ccgcgcatgt tgaggagcag
     2101 ggcggacaca gcagcttctc tagcatgctg tgccccggcg tccagggcga tggcctctcg
     2161 gacctcagac gcgacccatc cggatgtcgt cttcgggcgt cgagctgatc ttgtggacgg
     2221 caaggtcggc gccttcgcgc tcctcctcga cggagaggcg cagaccgaac agggcgtcga
     2281 caatgccgta gatgatccaa ccggctatga aaccggcagc cgcccccatc aggctgccga
     2341 cgagttgcgc ctgaaaagtg acgccgccca tgccgccgag cgccttcagc ccgaagatgc
     2401 cgcaggcaat gccgccccac agcccgcaga gcccgtgcag cggccagacc ccgagtacat
     2461 cgtcgatctg ccactcgttg gtggccttct ggaacatgac gacgaagatg ccgccagcga
     2521 cgccgccgac aatcagcgcg ccgatcgggt gcatcacgtc ggagccggcg cagaccgcga
     2581 ccagccccgc gagcgcaccg ttatgcacga agcccggatc attgtcgccg acgaccatgg
     2641 cgacgatgat gccgccgcac atggcgagca gcgagttcat ggcgacgagg cccgagacgc
     2701 tcttgagcga ttgcgcgctc atcacattga agccgaacca gccgacacag agcatccacg
     2761 agcccatggc gagccagggg atcgacgagg gcggtatgcc gaacgagcga cccttgtcat
     2821 agcgcccgag ccgcgggccg aggcgccgga tggcggcgag ccccgcccat ccgccgaagg
     2881 catgcaccac gatcgagccg gcgaagtcct tgaactcggc gccgagcatt ccggcgaaca
     2941 gcttatcctg cagcccgaag ttcttgttcc acacgatgcc ttcgaagagc gggtagccga
     3001 gacccacgat gagagcggtc gcgaggcact gcggcatgaa gcgcgcccgc tccgcgatgc
     3061 cgccagagat gatcgccggg atcgccgcgg cgaacgtgac gaggaagaag aatttgacga
     3121 gcgagaggcc ttgcgcgtcg tagcctgcgg cgccacccga gatcgcctcg gcgctcgcga
     3181 agaacaggat gccgtaggcg atccaatagc cgatgaagaa gtaggcgagt gtcgagaggg
     3241 cgaagtcgac gatgatcttg cagagtgcgt tcacctggtt cttgcgcctt acggtgccga
     3301 cctcgaggaa cgcaaacccg ccatgcatcg cgaacacgag aatcgcgccg agcagcagga
     3361 acagcacatc cgcgccggcc tgcagcccct gcaggccctt ctgtagctct gccatcgaag
     3421 cccccgaagt gattattttt taggcaatta ggcgctattc agccagtatg cctgctcggt
     3481 ggacgataag atcaagaatt gtgccagctc ggtaatcaat tttttaccaa tttgaatttg
     3541 ccgaataata tggccacgag tgaaccggcg actgcgccgc gccttctcct ggcatgactg
     3601 aattttgatc atcaatgacg cgcgtctggc caaatattag gcacggattt gcctgccgct
     3661 cggtgcactg tggcgacaac gctactatcg tccaacacat gctctcgcgc tggagattgc
     3721 cggctttgcc gcagcgcgat aaagaacgtt tttgcagtgt cacgccgccg gggagccgag
     3781 cacatgtcga atgccacacc gcaacgacga cccgtcgagc ggccgatctc gccgcacctc
     3841 caggtgtacc ggctgcagat ccatatgctc atgtcgggcc tgcatcgcgt cacgggcgct
     3901 gccctctacg ttggcacagc cttgctcgcc tggtggctga tcgcagtcgc ctcggggccg
     3961 cgctacttcg aatacgtgaa cggcatcctc ggacatccga tcggcaagct cattctgttc
     4021 ggctacacct gggcgctcgt ccaccacatg gtgggcggag tccgccacct tatctgggac
     4081 accggccgcg gctttgatct cggcacggtc aagaccatgt cctggttgac cattatctcg
     4141 tcggtggtgc tgaccctgct cctctggggc gtcggcctgt tcctgcgcgg agcgttctga
     4201 gccatgagca tggaaacacc actctccaag gtccggggtc ttggctccgc gcgtgaaggc
     4261 acgatgcact tttggcgtca gcgtgtgacg gcggtcgcca acgtcatcct ggtcacctac
     4321 ttcctgctgg tcctgctcaa gtatgccggt tcggactacg ccaccatgcg tgccgcgctg
     4381 cgcaatccgc tggtggcgat tccgctcctg ctgctggtcc tctccggcat cgtgcacgcg
     4441 aggctcggca tgcagacgat catcgaggac tacgtccacg gcgagggccg caagatcctc
     4501 atgctcatgg cgagcacgtt cttctcggcg ctggtcggcc tgacggccgt ctacgccatc
     4561 gtcaagatcg ccatcggcgc ctgagccgcc gccgagctta cggcatctct acgcgcacat
     4621 cagatccgca ccgccacgac aacaaggtca cccgagatgg caaagaacgg caccgcaaac
     4681 ggcgtggcga gccccgcagt caacggccgc gcctacccga ttaccgagca cacctatgac
     4741 gtggtcgtcg taggtgccgg cggcgcggga ctgcgcgcca cgctcggctg tgctgaggcc
     4801 ggtctgaaaa cggcctgcat caccaaggtc ttcccgacgc gctcgcacac ggtcgccgcg
     4861 caaggcggtg tcgcggccgc actcggcaac atgggccgcg acgactggcg ctggcacatg
     4921 tacgacaccg tgaagggcgc cgactggctc ggggaccagg atgcgatcga gtacctctgc
     4981 cgcaacgcgc ctgaggccgt ctacgagatg gagcactacg gcgtgccctt ctcgcggacc
     5041 gaggatggcc gcatctacca gcgcccgttc ggcggcatga cgaccgagtt cggcgagggc
     5101 ccgccggcgc agcgcacctg cgccgctgcc gaccgcaccg gtcacgccat gctgcacacg
     5161 ctctatggcc agtcgctccg ccatgccgcc gagttcttcg ttgagtactt tgcgatcgac
     5221 ctcataatgg acgccgaggg cgcctgccat ggcgtcgtgg cgctctgcct cgaggacggc
     5281 acgctgcacg ccttccgcgc gcacaagacg gtgctcgcga ccggtggcta tggccgcgcc
     5341 tacttctcct gcacctcggc gcacacctgc accggcgacg gcaacgccat ggtcgcccgt
     5401 gccggcctcg ccctgcagga ccacgagttc atccaattcc atccgacggg gatctacggc
     5461 tccggctgcc tgatcaccga gggcgcgcgc ggcgagggcg gctacctcac c
//
LOCUS       DAORNH010000309         5485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11014_length_5485_cov_2.886740, whole genome shotgun sequence.
ACCESSION   DAORNH010000309 DAORNH010000000
VERSION     DAORNH010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11014_length_5485_cov_2.886740"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PK264_21865"
     CDS             <1..849
                     /locus_tag="PK264_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78551.1"
                     /translation="WLGGSPVMIKEGGKDVPLWLQNAGYIWVPFIVVSAFAAWFGMND
                     LASAKASFAEQSVIFQRKHNWIMCWLYTGTFGSFIGYSAGFPLLAKTQFPEVNALAYA
                     FLGPLVGAVSRSLTGWVSDKWGGGRVTFWVFIGMAAGVSGVLYFLTVKGWPGFFISFL
                     FLFFMSGVGNASTFQMIPAIMRREMDRLMPGTTASERTRQAEKESAAIIGFTSAIAAY
                     GAFFIPKGYGTSIALTGSPQAALVGFLLFYVSCIAATWWFYTRPGGLLHDIERGGTTP
                     AAQPAA"
     gene            880..1850
                     /locus_tag="PK264_21870"
                     /pseudo
     CDS             880..1850
                     /locus_tag="PK264_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363708.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     assembly_gap    1851..1867
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1868..>4388
                     /locus_tag="PK264_21875"
     CDS             <1868..>4388
                     /locus_tag="PK264_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HRD78552.1"
                     /translation="RQYTDMPMLVRLVEQNGQLVPDRQLRASDFHGALGETNNPDWKT
                     VAFDEARGEIVVPRGSVGFRWGEQGKWNLEEKESGGADTSLRLSLGPIKDDVAEVAFP
                     YFGNIAHEHFASTAHASVLKRRVPVKRVTLKTGEALVASVYDLFLANYGVDQGFGGGN
                     VARSYDEDTPYTPAWAEKITGVSRDKIIQVAREFATNAEKTNGRSMVIIGAAMNHWYH
                     ADMNYRGVINMLVMCGCVGQSGGGWSHYVGQEKLRPQAGWAPLAFALDWGRPPRQQNS
                     TSFFYTHTDQWRYETIATAEIVSPTAPPGPWDGAIIDYNIRSVRMGWLPAYPTLKQNS
                     LAIARDAAAAGMEPPAYVAKALKEGKLSFAWEDPDSPENFPRNLFVWRSNLLGSSGKG
                     HEYFLKHLLGTSHGVLGKDLGEEGREKPREVAWHEEAPRGKLDLLVTLDFRMSTTCVY
                     SDIVLPTATWYEKNDLNTSDMHPFIHPLTNAVDPVWEAKSDWEIYKAIAKAFSRVAPE
                     VLGVEKDVVLTPIMHDAPAEIAQPFEPKDWKRGECEPIPGKTMPAVAVVERDYPNVYK
                     RFTALGPLMEKVGNGIKGIAWKTEHEVESLKQLNGVVTDEGPTRGMAKIDTDIDAAEV
                     LLTLAPETNGEVAVRSWASLSKTTGRNHVHLAAVKEDEKIRFRDVVAQPRKIISAPTW
                     SGLESEKVCYNAGYTNVHELIPWRTLSGRQQLYQDHLWMRAFGEALCVYRPPVDLKTI
                     KGIQGLKPNGEKEVVLNFITPHQKWGIHSTYTDNLLMLTLSRGGPIVWLSENDAAKAG
                     IVDNDWVEAYNANGALVARAVVSQRMKDGTLFMYHAQEKIVN"
     assembly_gap    4389..4447
                     /estimated_length=59
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4643..5065)
                     /locus_tag="PK264_21880"
     CDS             complement(4643..5065)
                     /locus_tag="PK264_21880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HRD78553.1"
                     /translation="MAIDRIVFDTNNLISAAISPLGKPFAAVRWIVERGRPVTSKPLL
                     DEVTRRLRRPRIVRVISEPHATELLRDFLAVALLVEPAPLPPTCRDPDDDVVLATALA
                     AGADCIVTGDEDLLVLDPFRGIRILTPAAFMDAVAAAD"
     gene            complement(5055..5336)
                     /locus_tag="PK264_21885"
     CDS             complement(5055..5336)
                     /locus_tag="PK264_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78554.1"
                     /translation="MVKLQATKVPIDVEALAEAARHAPVTVMNGDKEAFVVVEPAEFK
                     RLVEAGERARRKDAAARLLATMDRIHRDVETRASPEEIAELLRELEHGD"
BASE COUNT          970 a         1817 c         1725 g          897 t
ORIGIN      
        1 tggctcggcg gcagcccggt catgatcaag gaaggcggca aggacgtgcc gctctggctg
       61 cagaacgcgg gctacatctg ggtgccgttc atcgtcgtct cggcgtttgc cgcctggttc
      121 ggcatgaatg atctcgcctc tgccaaggcc tcctttgccg agcagtcggt gatcttccag
      181 aggaagcaca actggatcat gtgctggctc tataccggca cgttcggatc gttcatcggc
      241 tactcggcgg gcttcccgct gctcgcgaag acccagttcc ccgaggtgaa cgcgctcgcc
      301 tacgcgttcc ttgggcccct cgtcggcgcg gtctcgcgct cgctcacggg ctgggtctcg
      361 gacaaatggg gcggcggacg cgtcaccttc tgggtgttca tcggcatggc ggcgggcgtc
      421 tccggcgtcc tttacttcct caccgtgaaa ggctggccgg gcttcttcat ctccttcctg
      481 ttcctgttct tcatgtcggg cgtcggcaac gcgtcgacct tccagatgat cccggcgatc
      541 atgcgccgtg agatggatcg cctgatgccg ggcacgacgg cctccgagcg cacgcggcag
      601 gcggagaagg agagcgccgc catcatcggc ttcacgtcgg cgatcgccgc ctacggcgcc
      661 ttcttcatcc cgaagggcta cggcacttcg atcgccctca cgggaagccc gcaagcggcc
      721 ctcgtcggct tcctcctctt ctatgtgagc tgcatcgcgg cgacgtggtg gttctacacg
      781 cggccgggcg ggctcctgca cgacatcgag cgcggcggca cgacgcccgc agcccagccc
      841 gccgcctgat cagcgccacg ccgaattcgg agcaagtcca tgagccactt tctcgaccgc
      901 ctcactttct tccggcaggc ccgcgagccg ttcgccaacg gtcacggcgt cacgaccaac
      961 gaggatcgcc gctgggagga cggctatcgc cagcgctggc agcacgacaa ggtcgtccgc
     1021 tcgacgcacg gcgccaactg cacgggttcg tgctcgtgga agatctacgt gaagggcggc
     1081 atcgtcacct gggagacgca gcagaccgac tatccgcgca cgcggcccga tctgccgaac
     1141 cacgagccgc gcggctgctc gcgcggcgcc tcttacagtt ggtatctcta ctcggggaac
     1201 cgggtgaagt atccgctgat ccgctcgcgg ctgctccgcc actggcgtga ggcgagaaag
     1261 acgctcgccc cggtcgcggc ctggaagtcg atcgtcgaaa acccgaagaa gcgcgccgac
     1321 tacatgcagg tgcgcggcca cggcggcttc gtgcgcgcga agtgggacga ggtgaccgag
     1381 ctcatcggcg ccgccaacgc ctacacggcg aagacctacg gccccgaccg ggtcatcggc
     1441 ttctcgccga tcccggccat gtcgatggtc tcctatgcgg cgggctcgcg ctatctctcg
     1501 ctcatcggcg gcgtgtgcat gtcgttctac gactggtatt gcgacctacc gccggcatcc
     1561 ccgcagacct ggggcgagca gacggacgtg cctgaatcgg ccgactggta caacgcgggc
     1621 ttcatcatcc tgtggggctc gaacgtgcct cagacgcgca cgcccgacgc gcatttctat
     1681 accgaggcgc gctaccgcgg caccaagtcg gccgtgatct gccccgacta ctcggaggcc
     1741 gccaagttcg gcgatatctg gctctcggtg aagcagggca ccgacgccgc gctcgccatg
     1801 gccatgggcc acgtgatcct caacgagtat cacgtgaagc ggcaggcgaa nnnnnnnnnn
     1861 nnnnnnnccg ccagtacacc gacatgccga tgctcgtccg tctcgtcgag cagaacggcc
     1921 agctcgtgcc tgatcggcag ctgcgggcct ccgacttcca tggtgcgctc ggcgagacca
     1981 acaaccccga ttggaagacc gtcgccttcg acgaggcgcg cggcgagatc gtcgtcccgc
     2041 gcggctcggt cggcttccgc tggggcgagc agggcaagtg gaacctcgag gagaaggagt
     2101 caggcggcgc cgacacctcg ctccgcctct cgcttggtcc catcaaggac gatgtcgccg
     2161 aggtcgcgtt cccctatttc ggcaacatcg cgcacgagca cttcgcctcg accgcccatg
     2221 caagcgtgct gaagcgtcgt gtgccggtga agcgcgtcac gctcaagacc ggcgaagctc
     2281 tggttgcgag cgtctacgat ctcttcctcg ccaactacgg cgtcgaccag ggcttcggcg
     2341 gcggcaatgt cgccaggtcc tacgacgagg acacgcccta cacgccggcc tgggcggaga
     2401 agatcacggg cgtctcacgc gacaagatca ttcaggtcgc gcgcgagttc gcgacgaacg
     2461 cggaaaagac caacggccgc tcgatggtga tcatcggcgc cgccatgaac cactggtacc
     2521 atgccgacat gaactatcgc ggcgtgatca acatgctcgt catgtgcggg tgtgtcggcc
     2581 agtctggcgg gggctggtcg cactacgtcg gccaggagaa gcttcggccg caggccggct
     2641 gggcaccgct cgccttcgcg ctcgactggg gccgtccgcc gcgccagcag aactcgacgt
     2701 cgtttttcta tacgcatacc gaccagtggc gctacgagac gatcgcaacg gccgagatcg
     2761 tctcgccgac ggcgccgccc gggccgtggg acggcgccat catcgactac aacatccgct
     2821 ccgtccgcat gggctggctg ccggcctatc cgacgctcaa gcagaactcg cttgccatcg
     2881 ccagggacgc tgccgccgcc ggcatggagc cgccggccta tgtcgcgaag gcgctgaagg
     2941 aggggaagct ctcctttgcc tgggaggacc cggacagccc cgagaatttc ccgcgcaacc
     3001 tgttcgtgtg gcgctcgaac ctcctgggct cgtcgggcaa gggccacgag tatttcttga
     3061 agcatctcct cggcacttcg cacggggtcc tcggcaagga tctcggggag gagggacgcg
     3121 agaagccaag ggaggtcgct tggcacgagg aggcgccgcg cggcaagctc gacctgctcg
     3181 tgacgctcga ctttcgcatg tcgacgacgt gcgtctactc cgacatcgtg ctgccgacgg
     3241 ccacctggta cgagaagaac gatctcaaca cctccgacat gcaccccttc atccacccgc
     3301 tgaccaatgc ggtcgatccg gtgtgggagg cgaaatcgga ctgggagatc tacaaggcaa
     3361 tcgccaaggc gttctcgcga gtggcccccg aggtgctcgg cgtcgagaag gacgtggtgc
     3421 tgacgccgat catgcacgac gcgcccgccg agatcgcaca gcccttcgag ccgaaggact
     3481 ggaagcgcgg cgaatgcgag ccgatccccg gaaagacgat gccggccgtc gccgtggtcg
     3541 agcgcgatta tccgaacgtc tacaagcgct ttacggcact cgggccgctc atggagaagg
     3601 tcggcaacgg catcaagggc atcgcgtgga agacggagca cgaggtcgag agcctgaagc
     3661 aattgaacgg cgtcgtcacc gatgagggtc cgaccagggg catggcgaag atcgacacag
     3721 acatcgacgc cgccgaggtg ctcctgacac tcgcgcccga gaccaatggc gaggtcgcgg
     3781 tcagatcgtg ggcctcgctc tccaagacga cggggcgcaa tcacgtccat ctcgccgcgg
     3841 tgaaagagga cgagaagatc cgcttccgcg acgtcgtcgc gcagccacgc aagatcatct
     3901 cggcgccgac ctggtccggc ctcgagagcg agaaggtctg ctacaacgcc ggctacacga
     3961 acgtgcacga gctcattccg tggcgcacgc tctccggccg ccagcagctc taccaggatc
     4021 acctctggat gcgcgccttt ggcgaggcgc tctgcgtcta tcgcccgccc gtggacctca
     4081 agaccatcaa gggcatccag ggcctgaagc cgaacggcga gaaggaggtc gtcctcaact
     4141 tcatcacgcc gcaccagaag tggggcatcc actcgaccta tacagacaac ctcctgatgc
     4201 tgaccctttc gcgcgggggc ccgatcgtct ggctctcgga gaacgacgcg gcaaaagccg
     4261 gcatcgtcga taacgactgg gtcgaggcct acaacgccaa cggcgcgctc gtcgcccgcg
     4321 cggtcgtctc gcagcgcatg aaggacggca cgctcttcat gtaccacgcg caggagaaga
     4381 tcgtgaacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnncgg tgctcaagcc gacgcacatg atcggcggct acgtccagca ggcctacggc
     4501 ttcaactact acggcaccat cggcaccaac cgcgacgagt tcgtggtggt gcggaaggtg
     4561 cgcaaagtcg attggctgga gcggccgtac agccgtgggc aggaggcggc cgaatgagca
     4621 cgcgctcaga ggtgtcgcgg catcaatccg cagccgcgac cgcatccatg aaggccgcag
     4681 gcgtgagtat gcggatgccg cggaacgggt cgaggaccag caggtcctcg tcgcccgtga
     4741 cgatgcagtc ggcgcccgcc gccagtgccg ttgcgaggac gacgtcatcg tccggatcgc
     4801 ggcaggtcgg ggggagggga gcgggctcga ccaacaaagc caccgccaga aagtctcgca
     4861 gcagctctgt agcgtgcggc tccgagatca cgcgtacgat ccgtgggcgg cgcaggcgcc
     4921 tcgtcacctc atcgagcaat ggcttcgagg tcacgggccg accccgctcg acgatccagc
     4981 gaaccgcggc gaagggcttt cctagcggtg aaatggcagc gctgatcagg ttgttcgtgt
     5041 caaagacgat ccgatcaatc gccatgctcg agctcgcgca acagttcagc gatttcttcc
     5101 ggacttgctc gcgtttcgac atcgcgatgg atgcgatcca tcgttgccag cagtctcgca
     5161 gcggcgtcct tccgtcgcgc ccgctcgccc gcttcgacca gccgcttgaa ctctgccggc
     5221 tcgacgacga cgaacgcctc cttgtcgccg ttcatgacgg tgacgggcgc atgccgcgcg
     5281 gcttccgcca gcgcttcgac gtcgatcgga accttggttg cctgcagctt caccatcgga
     5341 aatctcctcg ctgccaaact agcacaacgg tcgtgcgaac ggaaatttcc gcatcgacat
     5401 ccgccgacag agcccccagg agcgccccat gaaagttcgc gcacagatcg cgatggtcct
     5461 gaacctcgac aaatgcatcg ggtgt
//
LOCUS       DAORNH010000310         5477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11035_length_5477_cov_2.623755, whole genome shotgun sequence.
ACCESSION   DAORNH010000310 DAORNH010000000
VERSION     DAORNH010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11035_length_5477_cov_2.623755"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..710)
                     /locus_tag="PK264_21890"
     CDS             complement(144..710)
                     /locus_tag="PK264_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78555.1"
                     /translation="MRRLPLASSLAAAALAAALVPAFAAANPQKSVYSTIDLKRDCRI
                     VKKHQDGNSWSCKGLTGYPLYVAEGDLRFFVSAGASPEKRRAATQTLAGFNSIFKDDK
                     TNRATLEWRVANPGPKATPYATILRYYLSVDGKKWQTLVVMKVTPTETCHVAHVDAAL
                     PNAVAVAREIADGAAAFDCTKEPVERKK"
     gene            complement(1246..2616)
                     /locus_tag="PK264_21895"
     CDS             complement(1246..2616)
                     /locus_tag="PK264_21895"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD78556.1"
                     /translation="MGSVEARAADLGGDCCADLEERVAELEATTVRKGNRKVSLTVSG
                     FVAHNVMFWDDGTQSDMYIGDGGNYGSRFRFVGTAKITPSVTAGFLYEFGVANNGLNA
                     MNQLNGGDDLGSSFALRDSTVWLRHSQLGMIKVGHGSTATDNLVLIDLSGCSGACTPD
                     IALYNGGFLLRGNNGVLGNAATVPLPAAGGGTAGMWAAAIRGHESWDTSRRNHILYEA
                     PTLAGFTLQMAVAEDNYWDIALRYAGEHHGFRIAGGIGYQHDSEFNFGTGGPGLATVL
                     CTAHCDTVSEEIKGALSVLHLSTGLFATISAGKRELSGGSPRPLTGGGPGFEFATNPT
                     PDLTYWHLIAGISSNHFGIGRTVLFGEYGEHKGGLAQSQFMSNTGGCNPTTGTLLHDC
                     TSNSKVTYWGLGVTQHIDAAAMELFLTYKNYSLDASGFAAGNAHLNNGGAADFSAVII
                     GTRINF"
     gene            complement(2983..4323)
                     /locus_tag="PK264_21900"
     CDS             complement(2983..4323)
                     /locus_tag="PK264_21900"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRD78557.1"
                     /translation="MTARWLQASVSGTALLAAALMIVPGSNAQAADLGGNCCADLEER
                     VAELEATTVRKGNRKVSLTISGFVAHNVMFWDDGVQSDMYIGDGGNYGSRFRFVGTAK
                     ISPNVTAGFLYEFGVANNGLNAMNQLNGGDDLGSTFALRDSTVWLRHNQLGMIKVGHG
                     STATDNLILIDLGGLSGATTPDIALYNGSFILRNPVTSLGFPIVSLTWDQLILGRQES
                     YDTSRRNHVLYETPSLAGFTLQAAVAEDNFWDVALRYAGEFGGFRLAAGIGYRQDSEF
                     NQTNQLLNVGGALCASQCERVQSVLLGSASILHVPTGLFVTGAAGSREDENFLSLIGA
                     TDNRNAKSSFWHLTAGISNNFTGLGRTVFYGEYAEHNDALRHFFSDVTGSDVKYWGLG
                     VVQHIDAAAMEIFIAYKNYDASFTRNVGTLAAPVIESVNFDAFSTVIVGTRINF"
     gene            4799..>5477
                     /locus_tag="PK264_21905"
     CDS             4799..>5477
                     /locus_tag="PK264_21905"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD78558.1"
                     /translation="MRDALILVIGTGPAGLAAAVALAERGATVQAVGPRPLASAARDT
                     RTAALFGPSVTFMRSIGAFDRLEGASEPITGIRLIDDTGRLLRAPDALFKARDVGRQA
                     FGLNCPNAAIVEALVSRAGTLPTLSLINDSVSAIEPRAEHVAVILASGRTLEARLVVG
                     ADGRGSPSRIAAGISATTWSHPQTAIVTSFAHSRPHGGISTEFHRASGPFTTVPLKGD
                     RSSLVWVE"
BASE COUNT          973 a         1758 c         1760 g          986 t
ORIGIN      
        1 gaagagcttc atgccgaagg gcgggttgcg cagcttgagg atgcgctcga ggtcggcggc
       61 gcggcgggca agatcggtcg ccatggggct ggttcctggc atgggctgcg gacggcaggc
      121 gggcgagggt cgagcgagtg ctgctacttc ttccgctcga ccggctcctt ggtgcaatcg
      181 aaggctgcgg ctccatcggc gatctcgcga gcgacggcca cggcgttggg cagcgcggcg
      241 tccacatgcg cgacgtgaca ggtctccgtc ggcgtcacct tcatgacgac gagcgtctgc
      301 cacttcttgc catcgacgct caggtagtag cgcaggatcg tcgcgtaagg cgtcgccttc
      361 ggaccgggat tggcgacgcg ccattcgagc gtcgcgcggt tggtcttgtc gtccttgaag
      421 atcgagttga agccggcgag cgtctgcgtc gccgcgcggc gcttctcggg actggcgcca
      481 gcgctcacga agaagcgaag atcgccttcg gccacataga gcggatagcc cgtgagcccc
      541 ttgcacgacc aggagttgcc gtcctgatgc ttcttcacga tgcggcagtc gcgtttgagg
      601 tcgatggtcg agtagacgct cttctgcgga tttgccgcgg cgaatgccgg taccagggcc
      661 gccgcaagtg ctgccgcagc aaggctcgag gcaaggggaa gtcggcgcat gtcgtctccg
      721 ctggctctcc acgttcgcca aacggtaaac ctgcatccgg gtttgggcaa tgcgggagac
      781 ggcgccgcgg cagcgacgca cactccgcct cgtcatgccg gcgtcgtgca ttgcctcccg
      841 gagcagccgg atagggctgc tggagccggc tccgcgcgac actgtcatgt gtggacgcct
      901 gaacggcact cctgccgcaa aggcgctctg ggacccagcg aagaaagcag cgcaactgcc
      961 gcattccttc cgcccgcact accgagcgct ggatcccggc acggcgcggt cgcgtgcgct
     1021 cgcgcttggc tgcgatgatg acagggggtg gccactccga ggtgaccctg atgccggcca
     1081 tccccggcat gacggtgtgg gtggggggtt gcctctggct cgcggttccg tcaccaggcc
     1141 ccacagtgga cttccacctc cagcagcaat gcctggtaca caaaagagcc gcccgatggg
     1201 cggccctttc aggtcgagca tggtccttcg ccgttcggcg aagcctcaga agttgatgcg
     1261 cgtaccgatg atgacggccg agaagtcggc agcgccgcca ttgttgaggt gggcattgcc
     1321 ggcggcgaag cccgaggcgt cgagcgagta gttcttgtag gtgaggaaca gctccatggc
     1381 cgccgcatcg atgtgctgcg tcacgccgag accccagtag gtgaccttgc tgttcgacgt
     1441 gcagtcgtga aggagcgtgc ctgtcgtcgg gttgcatcca cccgtattgg acatgaactg
     1501 cgactgcgca aggccaccct tgtgctcgcc atactcaccg aagagcaccg tgcggcctat
     1561 gccgaagtga ttgctcgaga taccggcgat gaggtgccag taggtgagat ccggtgtcgg
     1621 gttcgttgcg aactcgaacc cgggcccacc gccagtgagc ggcctcgggc tgccgccaga
     1681 aagctcgcgc ttgccagcgg agatcgtcgc gaagaggccc gtcgagaggt gcagcacgga
     1741 gagagcaccc ttgatctcct cggagactgt gtcgcaatgg gccgtgcaca gcaccgtcgc
     1801 caggcccggt ccgcccgtgc cgaagttgaa ctcgctgtcg tgctggtagc cgatgccgcc
     1861 agcgatgcga aagccatgat gctcgcccgc atagcgcagg gcgatatccc agtagttgtc
     1921 ctcggcgacc gccatctgca gcgtgaagcc cgcgagcgtg ggcgcctcgt agaggatgtg
     1981 attgcggcgc gaggtatccc acgactcgtg ccctcggatc gccgcggccc acatgcccgc
     2041 cgtgccgccg ccagcggccg gcagcggaac agttgcggcg ttaccgagta ctccattgtt
     2101 gccgcgcagc aggaagccgc cgttgtaaag cgcgatatcc ggagtgcagg cgccgctgca
     2161 gcccgacaga tcgatcagca cgaggttgtc cgtcgcggtc gagccgtggc cgaccttgat
     2221 catgccgagc tggctgtggc gcagccacac ggtcgagtcg cgcagcgcaa aggagctgcc
     2281 gagatcgtca ccgccgttca gctggttcat ggcgttgagg ccgttattgg ccacgccgaa
     2341 ctcgtagagg aagccggcag tcacgctcgg cgtgatcttc gctgttccga cgaagcggaa
     2401 gcgcgagccg tagttgccgc cgtcaccgat gtacatgtcg gattgggtac catcgtccca
     2461 gaacatcacg ttgtgggcaa cgaaccccga aaccgtgagc gacaccttgc gattgccctt
     2521 gcggacggtt gtcgcctcga gttcggcaac acgctcctcg agatcagcgc agcagtcgcc
     2581 gccgaggtca gcagctctcg cttccacgct tcccacgatc aatccggctg acgcgataag
     2641 cgccgcaagg ctcgctacat tccatgtccc gctgaattgc atctggtccc cacgctgttc
     2701 cgccgcccgt agattggcat tgcgcaagat cgcggccacg tcttgctggg ccctgcccac
     2761 acgcgaaaca aatattggcg aagcactgga acgaatcggc ttcgagccct gtagttgtcg
     2821 cctacgccaa ttgcggcatc acggtccaac caagacgtcg ctcgattgag ctttgctcca
     2881 cagcctgtac cggtttggtc acgtcctgcc tcacaaaaca gaagagccgc ccggaggcgg
     2941 ctctcaacgt ttccgtcgcg gctctcgccg gtcagatcag catcagaagt tgatgcgcgt
     3001 gccgacaatg accgtcgaga aggcgtcgaa attgaccgac tcgatcaccg gggccgccag
     3061 cgtgccgacg ttgcgcgtga acgaggcatc gtagttcttg taggcgatga agatctccat
     3121 cgcggccgca tcgatgtgtt gcacgacgcc gaggccccag tacttcacgt cgctcccggt
     3181 cacatcgctg aagaagtgtc gcaaggcgtc gttgtgctcg gcgtactcgc cgtagaacac
     3241 cgtgcggccg aggccggtga agttgttcga gataccggca gtcagatgcc agaagctcga
     3301 cttcgcgttg cgattgtctg tggctccgat caagctcagg aagttttcgt cctcgcgcga
     3361 accggcggcg ccggtcacga agaggccggt cggcacgtgc aggatcgacg ccgatccgag
     3421 caggaccgac tgcacccgct cgcactgaga ggcgcaaaga gcaccgccaa cattcagcag
     3481 ctgattcgtc tggttgaatt ccgaatcctg ccgatagccg atgccggcgg ccaggcgaaa
     3541 gccgccgaac tcgcctgcat agcgcagcgc cacgtcccag aaattgtcct cggcgacggc
     3601 cgcctgcagc gtgaagccgg caagtgatgg cgtctcgtag aggacgtgat tgcggcgcga
     3661 cgtatcgtag ctttcctgac ggccgaggat gagctggtcc caggtcaagc tgacgatggg
     3721 gaaaccaagg ctcgtcaccg gattgcgcag gatgaacgag ccattataga gggcgatgtc
     3781 cggggtcgta gcgccgctga ggccgccgag atcgatgagg atgaggttgt ccgtcgcggt
     3841 cgagccgtgg ccgaccttga tcatgccgag ttgattgtgg cgcagccaga cagtcgagtc
     3901 gcgcaacgcg aacgtcgagc cgagatcgtc gccgccattg agctggttca tggcgttgag
     3961 gccgttgttg gccacgccga actcgtagag gaagccggcc gtgacattcg gcgagatctt
     4021 cgcggtgccg acgaagcgga agcgcgagcc gtagttgcca ccgtcaccga tgtacatgtc
     4081 gctctggacg ccatcgtccc agaacatcac gttatgggca acaaagccgg agatcgtcag
     4141 cgacaccttt cggttgccct tgcggacggt ggtcgcctcg agctcggcga cgcgctcctc
     4201 gaggtcggcg cagcagttgc caccgagatc ggcggcctgg gcgttggagc ctgggacaat
     4261 catcagggcc gccgccagta gcgccgtccc gctaacactt gcctgcaacc atctcgctgt
     4321 catggtgagc tccttggaat tcaagcttga gtacgcttcg gtcgcccctg ccatttccgc
     4381 tactcaaata tccgccaact gcgacgaacg gaaatcctgg ccaggtgcag gcaacaactg
     4441 gtcggggaaa gctagggtcc cgccacgcca ctcgtcgaat ccaatcatcc aggcatcatc
     4501 gcttctcccc gaattcgttg ccgatccgca acaaaatgtt gaaacacttt ggatgtttga
     4561 cgcggggttg ccctggttag cctcatgtta acctcaacct ccggctgttc caacgcgtcg
     4621 gatcccgata aagttgcatt ctcgagctgg cgaatcgccg agcaactgaa ccaagtcttc
     4681 cggatatgac ccgaggtgat ccgcgcgatg gcatttggtc gtgagccaat gtgccccgcg
     4741 cgacctggca acgttcgatg ctacgaaggt caggcgccaa ttcgtccgga gtttcgccat
     4801 gcgcgacgca ctcatcctgg tgatcggcac cggtcccgcc ggcctggccg cggcggtcgc
     4861 cctggcggag cgaggcgcca ctgtgcaggc cgttggtccg cgccctctcg ccagtgccgc
     4921 acgagacacg cgcacggcgg ccctgttcgg cccaagcgtc acgttcatgc gctccatcgg
     4981 tgcattcgat cgcctggagg gggcgtcgga accaatcacc ggcattcgcc tcatcgacga
     5041 cacgggccgt ctgctgcgcg cgcccgacgc cctgttcaag gcgcgcgacg tcggccgcca
     5101 ggccttcggc ttgaattgcc ccaacgccgc gatcgtcgag gccctggtct cgcgcgcggg
     5161 cacgctcccg acactcagcc tgatcaacga cagcgtgtct gcaatcgagc cgcgcgcaga
     5221 acatgtcgcc gtgatcctcg cctccggacg gacactcgaa gcgcgcctcg tcgtcggcgc
     5281 cgatggccgc ggctcaccaa gccgcatcgc ggcggggatc agcgcgacaa cctggagcca
     5341 tccgcagacc gcgatcgtca catcattcgc ccacagccgc ccccatggcg ggatctcgac
     5401 ggagttccat cgggcgagcg gtcccttcac gaccgtgccg ctcaaaggcg atcgctcgag
     5461 ccttgtatgg gtcgagc
//
LOCUS       DAORNH010000311         5418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11206_length_5418_cov_2.666232, whole genome shotgun sequence.
ACCESSION   DAORNH010000311 DAORNH010000000
VERSION     DAORNH010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11206_length_5418_cov_2.666232"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK264_21910"
     CDS             complement(<1..548)
                     /locus_tag="PK264_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD78559.1"
                     /translation="MAKPPGVLIIVENLTVPLDRRVWQEATTLRDAGYTVSVVCPKGG
                     KYTAGYELLDGIHVFRHPLPFEADGALGYLIEYSSALFWELILSTRAYFKVGFDVVQA
                     CNPPDTIFMLAWLFKRLAGTPFIFDHHDINPELFEAKFGERGFFHRLLLKLERWTFRT
                     ADVSIATNDTFKQIAIDRGGMDP"
     gene            complement(701..1093)
                     /locus_tag="PK264_21915"
     CDS             complement(701..1093)
                     /locus_tag="PK264_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRD78560.1"
                     /translation="MPSEHEDRRRHSLAERRARYRRGHTSEWLAAAVLMAKGYRILAR
                     RYRTHGGEIDLVAVRGQRLAFVEVKRRRTRAEAEAAIEEVQSRRLGVAVDHWLARHAG
                     YREHEIGLDAVLVLPWRWPIHIENALAP"
     gene            1270..1971
                     /locus_tag="PK264_21920"
     CDS             1270..1971
                     /locus_tag="PK264_21920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD78561.1"
                     /translation="MTIFSTLRRAGLAAAAALAIGAPGGASAQNYDGSGIVRFGLFGQ
                     GTWVGSALVEENDTVAPPVLIARGSGDISGLGVGLSIGYDWRMHGNWIFGVEGDWTYV
                     GAESRWRTTTVTADYTGTLRARLGVYTHPHMLWYATGGLAMLGLEGEHTATTRDTAST
                     RFGWTIGAGTEINWHHVILFGEYLYADYEEWSFGVPANNITVDTDAHMFRLGIKFKVG
                     HDHYHDDVRHGRPMK"
     gene            2079..2834
                     /locus_tag="PK264_21925"
     CDS             2079..2834
                     /locus_tag="PK264_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD78562.1"
                     /translation="MQKLEGRVALVTGSAGGIGRAIADRLAREGAQVWIGDLDAAAAE
                     RAAAEIVAGGAKATAAALDVTKSQDVGATLGRIAADHGRLDILVNNAGLNVRSDFRHL
                     GEADWARIREVNLDGVVRLAREGFALLKASGRGAIINVSSIMSTRHLRQLAAYSATKG
                     AVTALTRALAVEYAPFAIRVNSVAPGFVETGLTERFLRNPAIKKALLDRTLLRRFGTG
                     ADMAGAVAFLASDDAAYITGAELVVDGGMSIGL"
     gene            2967..>4251
                     /locus_tag="PK264_21930"
     CDS             2967..>4251
                     /locus_tag="PK264_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78563.1"
                     /translation="MLRRYRMIGRRTILCLTWAAALAGVASVLPAIGQEAKPPANASP
                     GSDAFAPERVAGTYAAHLTGDFVVALEGGRLFLRLPLMPSAPLISRGGGRYEADHPQL
                     KGWSATFRPGSFSPDTIEMHIVQPEGQGVFPRRTTEPLERDLGEYSGLIGHYTAGKDK
                     AEILNLMGRLALYEHGSPAFPLKTAGRDVFIMTSAPGTGEFRVVVRREGGDVLGFVLR
                     QPDRTSEYLKAGAIQPVTSEQLRQKIIEALGGEAALKAKRTLVEEYELDLSGVTGTAK
                     TRWRAPLSNVSEISFVVPADKSRFTARSVFDGTKGSMISTGKPAKRHAQSDIDWEMIG
                     SPHEPLLWSRLYKSVVVLGPRRFDERDAIAVVKTLQSGRAVTAYYDATTYLPMGVEGL
                     KTNEGGREERAIQVQRDWRMVGGVRRPFTVLVREGE"
     assembly_gap    4252..4350
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4401..5177)
                     /gene="hisF"
                     /locus_tag="PK264_21935"
     CDS             complement(4401..5177)
                     /gene="hisF"
                     /locus_tag="PK264_21935"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRD78564.1"
                     /translation="MLKVRIIPCLDVKDGRVVKGVQFVDLIDAGDPVEAAAAYDAAGA
                     DELCFLDITASHEKRGIILDVVQRTAERCFMPLTVGGGVREVSDIRALLKAGADKVSV
                     NTAAVTRRAFVAEASEKFGAQCIVVAIDAKRVTPEGAPPRWEIFTHGGRNATGLDAIA
                     YAREVVALGAGEILLTSMDRDGTKAGFDLGLTRAIADAVPVPVIASGGVGTLDHLVDG
                     VREGRASAVLAASIFHFGTHTIGEAKRHMAKAGLAVRLDA"
     gene            complement(5221..>5418)
                     /locus_tag="PK264_21940"
     CDS             complement(5221..>5418)
                     /locus_tag="PK264_21940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD78565.1"
                     /translation="MCMASRPDARRSGGARAVLAAIEHWAGALGAGTLFLQTGADNAA
                     AQALYAGVGYGLAGRYHVRIA"
BASE COUNT          907 a         1749 c         1788 g          875 t
ORIGIN      
        1 gggtccatgc cgccgcggtc gatggcgatc tgcttgaacg tgtcgttggt cgcgatcgag
       61 acgtcggcgg tgcggaaggt ccagcgctcg agcttcagga gcaggcggtg gaagaagccg
      121 cgctcgccga acttcgcctc gaagagctcc ggattgatgt cgtggtggtc gaagatgaag
      181 ggcgtgcccg cgagccgctt gaagagccag gccagcatga agatcgtatc gggcgggttg
      241 caggcctgca cgacgtcgaa cccgaccttg aagtaggcgc gcgtcgacag gatcagctcc
      301 cagaagagcg cgctcgaata ttcgatgagg tagccgagcg cgccgtccgc ctcgaacggc
      361 aggggatggc ggaagacgtg gatgccgtcg agcagctcat agccggccgt gtacttgccg
      421 cccttcgggc agacgacgga gacggtgtag ccggcatcgc gcaaggtcgt cgcctcctgc
      481 catacgcgcc gatcgagcgg tacggtgagg ttctcgacaa tgatcagtac gcccggcggc
      541 tttgccatgg ctcaacgctc cccacacact gtcccggtgg cgtctgatgc gcctttccgt
      601 cagtcgatag tgcaggcaat tcgcgaacga tcggtgagag gacagggaca atgatccatg
      661 tccgtgcgtg ccgacatgag acagaggagc ggtgtgaggc tcaaggcgcg agggcgttct
      721 cgatgtggat cggccagcgc cacggcagca caaggactgc atcgagcccg atctcgtgct
      781 cccggtagcc cgcgtgccgt gcaagccagt gatcgaccgc gacgccgagc cggcgcgatt
      841 gcacctcctc gatggcggct tctgcctccg cgcgcgtccg ccgccgcttc acctcgacga
      901 atgcgagacg ctgcccgcgt acggccacga gatcgatctc gcccccatgc gtgcgatagc
      961 gtcgggccag gatgcggtag cctttggcca tgaggacagc agctgcgagc cattccgagg
     1021 tatggcctcg gcgatagcgg gcgcggcgct ccgcaagcga atggcggcgg cggtcctcgt
     1081 gctcgctagg catggcggcc tcgcacccct cgacactctc tgtgatgtgg cgacaacgcc
     1141 acggcacagc atgattaaca agaggttacg acttttcagc ccggcacctg cctcccccat
     1201 gttaaccatg gctatcagtg tcatgcgtcg aatcgtcaat gcgaacccag ccacgggact
     1261 tgcatcatca tgaccatttt cagcactctc cggcgggctg gcctcgctgc cgcggccgca
     1321 ctcgcgattg gtgcccccgg gggcgcgtcg gcccagaatt atgacggatc cggaatagtc
     1381 cgcttcggcc tcttcggcca gggaacgtgg gtcggttcgg cgctcgtcga ggagaatgac
     1441 acggtggcgc cgccggtgct catcgcgcgc ggctcgggcg atatcagcgg cctcggcgtc
     1501 ggcctgtcaa tcggctacga ctggcgcatg catggcaact ggatcttcgg cgtcgagggc
     1561 gactggacgt acgtcggcgc cgaatcacgc tggcgcacca cgacggtgac ggccgactac
     1621 accggcactc tgcgcgcccg gctcggcgtc tacacgcatc cccacatgct ctggtacgcg
     1681 accggcggcc tggccatgct tggcctcgag ggtgagcata cggcgacgac gcgcgatacg
     1741 gcctccacgc ggtttggttg gaccatcggc gctggcacgg agatcaactg gcaccacgtg
     1801 atcctgttcg gcgaatacct ctacgccgac tacgaggagt ggagcttcgg cgtgccggcc
     1861 aacaacatca cggtcgatac cgacgcccac atgttccgcc tcggcatcaa gttcaaggtc
     1921 ggccacgacc actatcatga cgatgttcgc cacggccgcc cgatgaagta agtacctggg
     1981 ttgagcaagc ggcacacttg atccggcgga ggcatccgcc ggatttcatt ttgtggcgcg
     2041 tcgactacaa gctgtgcggg atagcgcagg agctgagcat gcagaagctc gaagggcgcg
     2101 tggcgcttgt aacaggatcg gcgggtggca tcgggcgagc gatcgccgat cgcctcgccc
     2161 gcgaaggcgc acaggtgtgg atcggcgatc tcgatgcggc tgccgccgag cgggcagctg
     2221 ctgagatcgt cgcaggcggc gccaaggcaa ccgcggcagc tctcgacgtg acgaagagcc
     2281 aggacgtcgg tgccacgctc ggccgcattg ccgccgatca cggccgcctc gatatcctcg
     2341 taaacaacgc cggcctcaat gtgcgctcgg atttccgcca tctcggcgaa gcggactggg
     2401 cgaggatccg cgaggtcaat ctcgacggcg tcgtgcggct tgcacgcgag gggttcgcgc
     2461 tcctgaaagc gtcgggtcgt ggtgccatca tcaatgtgtc ctcgatcatg tcgacgcggc
     2521 atctgcgcca gcttgccgcc tactcggcga ccaagggcgc cgtaaccgca ctcacgcggg
     2581 cgctggcggt cgagtatgcg ccgttcgcga tccgcgtgaa ctcggtcgcc cccggcttcg
     2641 tcgagacggg gctcacggag cgctttctca ggaacccggc tatcaagaag gcgctgctcg
     2701 atcggacgtt gctccgccgt ttcgggacgg gcgcggatat ggcgggtgcg gtcgccttcc
     2761 tcgcgagcga cgatgccgcc tatatcacgg gcgccgagct cgtcgtcgac ggcgggatgt
     2821 cgatcgggct ctgatcggcc tcctgagacg cagccgccgc cgcagaaact gcgttgcggt
     2881 cctgtttccg cgctacttga cctgcgggaa caattccgtg agcattgtgg ctcgaggcag
     2941 ttcgcccccc cggaaaggct cagatcatgc tccgccgata tcggatgatt ggtcgccgca
     3001 ccatcctatg tctcacctgg gcagccgcac ttgcaggcgt cgcttcggtg ctcccggcca
     3061 tcggccagga agccaagccg ccggccaatg cgagccccgg cagcgacgcc ttcgcgcccg
     3121 agcgtgtcgc cggcacctat gcggcccacc tcaccggcga cttcgtcgtt gccctcgagg
     3181 gaggccggct gttccttcgg ctgccgctga tgccgagcgc gccgctgata tcccgcggcg
     3241 gcgggcgcta cgaggcggat catccacagc tcaagggctg gtcggcgacg ttccgcccag
     3301 ggagcttctc gcccgatacg atcgagatgc acatcgtcca gcccgagggc cagggcgtct
     3361 tcccgcggcg gacgacggaa ccgctcgagc gcgacctcgg cgagtacagc ggactgatcg
     3421 ggcactacac ggccggcaag gacaaagccg aaatcttgaa cctcatgggc cgcctcgcac
     3481 tttacgagca cggctcaccg gcgtttccac tgaagactgc cgggcgtgat gtcttcatca
     3541 tgacgagcgc gcccggcacc ggagaattcc gtgtcgtggt tcgtcgcgag ggcggcgacg
     3601 tgctcggatt cgtgctgcgc cagccggacc ggacgtccga gtacctgaag gcgggcgcga
     3661 tccagccagt gacgagcgag cagcttcgcc agaagatcat cgaggcattg ggaggcgagg
     3721 ccgccctcaa ggcgaagcgc acgctcgtcg aggagtacga gctcgatctc agcggcgtca
     3781 ctggcacggc gaagacgcgc tggagggcac ccttgtcgaa cgtgtccgag atcagcttcg
     3841 tcgtacccgc cgacaagagc cgcttcacag cccgctccgt gttcgacggc acgaaaggtt
     3901 ccatgatttc aacggggaag cccgccaagc gccacgcaca gtccgacatc gactgggaga
     3961 tgatcggatc gccgcacgag ccgctgctct ggagccgtct ctacaagtcc gtcgtcgtgc
     4021 tcgggccgag gcgcttcgac gagcgtgatg cgatcgcagt ggtgaaaacg ctgcagtcgg
     4081 ggcgcgccgt caccgcctac tatgatgcaa ccacatactt gccgatggga gtcgagggcc
     4141 tcaagaccaa cgaagggggc cgtgaagagc gagcgatcca ggtacagcgg gactggcgca
     4201 tggtcggcgg cgtccgccga ccgttcacgg tcctggtgcg tgaaggtgag gnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggccgaagc cgaagtgaat gcccgccaca
     4381 agtcggcggg gggggctggc tcaggcgtcg agcctgaccg caagcccggc cttcgccatg
     4441 tgcctcttcg cctcgccgat cgtgtgcgtg ccgaagtgaa agatggaggc ggcgagcacg
     4501 gcgctcgccc ggccttcacg cacaccgtcg acgagatggt cgagcgtgcc gacgccgcca
     4561 gaggcgatca ccggcaccgg gacagcgtct gcgatggctc gcgtcaggcc gagatcgaag
     4621 cccgccttgg tgccgtcgcg atccatggag gtgagcagga tctcgcccgc gccgagcgcg
     4681 acgacttcgc gtgcgtaggc gatggcgtcg agcccggtcg cgttgcgccc gccatgcgtg
     4741 aaaatttccc agcgcggcgg cgcgccctcc ggcgtgacgc gcttcgcatc gattgcgacg
     4801 acgatgcatt gcgcgccgaa cttctcggag gcctcggcga cgaaggcgcg ccgggtaacc
     4861 gccgccgtgt tgaccgagac cttgtctgca ccggccttca gcagcgcgcg gatgtcgctg
     4921 acctcgcgca cgccgccgcc gacggtcagc ggcatgaagc agcgctccgc tgtccgctgc
     4981 acgacatcga ggatgatgcc acgcttctcg tgactggccg taatgtcgag aaaacagagc
     5041 tcgtccgcgc cggctgcgtc ataggcggcc gcggcctcga ccggatcacc cgcatcgatc
     5101 aggtcgacga actgcacgcc cttgacgacg cggccgtcct tcacatcgag gcaggggatg
     5161 atgcgcacct tgagcatgtc gcgtcctctc ggctcgccga ccgcatttgc ctcgcggtcc
     5221 tcaggcgatg cggacgtgat agcgcccggc gaggccgtag ccaacgccgg catagagcgc
     5281 ttgcgctgcg gcattgtcgg cgccggtctg gagaaagagc gtacctgcac caagcgcacc
     5341 cgcccaatgc tcgatcgcgg ccagcaccgc gcgggcgccg ccggagcgcc gggcgtcggg
     5401 ccggctcgcc atgcacat
//
LOCUS       DAORNH010000312         5377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11306_length_5377_cov_2.533446, whole genome shotgun sequence.
ACCESSION   DAORNH010000312 DAORNH010000000
VERSION     DAORNH010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11306_length_5377_cov_2.533446"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="PK264_21945"
     CDS             <1..1407
                     /locus_tag="PK264_21945"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRD78566.1"
                     /translation="SAASEAELRNLLARANQILLDGIPATSERRAEWAVRTTTSIPVI
                     AATLNRAEISALASSGRVKRIWPDRVFGVELKESLGIVAGASPGGDLAAIEGADGRGT
                     TIAVIDSGIDTTHPFLAGRIVREACFSTPSGPGASSFCGTATTLKGAGAGKACTGIAS
                     CSHGTHVAGIVAGDGSSLGGSAPARGMAPKAKIMAIQVFHRVDSAEDCAPLAAPCTLA
                     STSDLLAAFDYILGEVAGLAEPLAAINMSVGGGEGTGPCNDTPLARVVQSLRARGVAT
                     VFSAGNSGFRNSTNDGTSCVEAAFSIGATTKDDLVSPFSNMSPIVDLLAPGSGIMSSV
                     IGGSFQAYDGTSFAAPHVAGAIAAIRSRHPRASLDAIEAALKVTGPRVSDQRPEGQHA
                     KPRIRVDLALRLLAGDANVAASRTRAAPSGTTTTTPKGNPAPAPGSATPATPGCDDVS
                     RRASGALTGGTGDAARCN"
     gene            1437..3020
                     /locus_tag="PK264_21950"
     CDS             1437..3020
                     /locus_tag="PK264_21950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRD78567.1"
                     /translation="MKFREIQTAKGWTTGRAIGAAASAAILAALAGSAVEAQTSDLGR
                     KSGTATARLIESQLAARGSARVIIMHDAPAGRADSADASRALLARATDTLLSGVPASD
                     ARRAEWSVNAASLMPVFAVTMNRAELNAVARSKNVVRIWPDQRNRFALDGSMPSIGLP
                     NPLPPAVAGADGKGATIAVLDSGVEAGHPFFNGRVVAEACFSANVKGESASLCPNKAN
                     TQTGAGSSAPCKAPFEGCEHGTHVAGIAAGKRSGNTGPAGGVAPGANILAVNIFSTNC
                     DAGQSCREISSWDSDGIAALNWVLEQHARIPGGVAAVNMSIGAGEFAGACDDRPYKVA
                     IDALRAAGIAVVVASGNSRFKNAAGSPGCVSSAVTVGSITKAGRISEFSNMSPIVDLM
                     APGTAIMSSVLNGRFESMDGTSMATPHVTGAFAVLKARFPRASVAELEEALRATGPAI
                     RDDRPGGTHSRPGIKVDLAIAHLAKTHTGVAATPPPATSTTPPPPVAKGPTPPNPCDT
                     TSAASSALTGGSADKAGCK"
     gene            3139..4614
                     /locus_tag="PK264_21955"
     CDS             3139..4614
                     /locus_tag="PK264_21955"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HRD78568.1"
                     /translation="MNAKPNTDLKRSPDRAAKAKVVAAAEAAETNLPIAEALAAFTMQ
                     TRAEDIPAEVRTRAVHHMLDAAGIAVAASRYDFSHRMLTGIRGLAGEGPVPVIGMPAR
                     LPARDAAMMNGFLCHGLDFDDTHVAAIVHPTASILPTVLSAGAMTGASGRDMVAAFVI
                     GVETAVRIGMVAKSGFHQVGFHPTGMVGVFGCTLAAGRLMGLNDAELRNAQGIALSMA
                     SGSLEFVADGAWNKRLHPGWAAHAGITAAALAREGFIGATKPYDGRFGLFPSYLGKES
                     DRADYPIATRGLGDTWELMATAIKPYPACHFTHACIDSALALRPQVGDWRRIKSIETL
                     VPEGVFKTVCEPEAGKLKPANSYDAQFSVQYLTAVALVRGKFGLAEIEPEAISNSDVL
                     ALTSKVKYGAYQDGVFPKLYHGEVVITLDDGRTLRHREHVNRGAAERPLTNGEIVEKF
                     NGNAARGFDRDTARRMEAAMLGLEKASNVDGVFAAFSPRLR"
     gene            4611..4994
                     /locus_tag="PK264_21960"
     CDS             4611..4994
                     /locus_tag="PK264_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD78569.1"
                     /translation="MIDHVSLSVSDMARSTRFYRAALEPLGYRCLTESSERAAFGKRY
                     PELWLNARPGMRPIEAATGAHICLRARTKSEIEAFHAGALAAGGTCDGPPGMRKALMV
                     DYFAAFVRDPDGNRIEAMTVPAAGA"
     gene            complement(5006..>5377)
                     /locus_tag="PK264_21965"
     CDS             complement(5006..>5377)
                     /locus_tag="PK264_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78570.1"
                     /translation="NLAVVEHMASRVAVMYLGRIVEEAPTDALFAHPKHPYTEALLKS
                     VLTPEPGRGIPDTNLGAAYPNPLDVPPGCSFHPRCGYKMPDCSVRAPRRSLVGQAAVE
                     CHLHDNAGAATDAPAREVVLS"
BASE COUNT          848 a         1905 c         1823 g          801 t
ORIGIN      
        1 tcggcggcga gtgaggccga actgcgcaac ctgctggcgc gcgccaacca gatcctgctc
       61 gacggcatcc cggcgacgag cgagcggcgc gccgagtggg cggtccgcac gacgacgagc
      121 attccggtta tcgcggcgac gctcaatcgc gccgaaatca gcgcgcttgc gagctcaggc
      181 cgggtgaagc gcatctggcc cgaccgcgtc ttcggcgtcg agctcaagga gagcctcggc
      241 atcgtcgccg gcgcgtctcc cggcggcgac ctcgcggcga tcgagggcgc ggacgggcgc
      301 gggacgacga ttgctgtgat cgattccggc atcgatacga cccatccgtt cctcgccggc
      361 cgcatcgtgc gcgaggcctg cttctcgaca ccgagtggtc cgggcgcctc ctcgttctgc
      421 ggcacggcga cgacgctcaa gggagccggc gccggcaagg cgtgcaccgg catcgccagc
      481 tgctcgcacg gcacgcatgt cgccggcatc gtcgcaggcg atggctcgag cctcggtggc
      541 agcgcgcccg cgcgcggcat ggcgcccaag gccaagatca tggcgatcca agtctttcac
      601 cgcgtcgaca gcgccgagga ctgcgcgccg ctcgccgcgc cctgcacact cgcgagcacc
      661 tcggatctcc tggcggcctt cgactacatc ctcggtgaag tcgcgggtct cgccgagccg
      721 ctcgctgcga tcaacatgag cgtgggcggc ggcgagggca cgggaccctg caacgacacg
      781 ccgctcgcca gggtagtgca gagcctccgc gcgcggggcg tcgcgacggt cttctccgcc
      841 ggcaacagcg gcttccgcaa ttcgaccaac gacggcacct cgtgcgtcga ggcggctttc
      901 tcgatcgggg cgacgaccaa ggacgacctc gtctcgccgt tctcgaacat gtcgccgatc
      961 gtcgatctgc tggcgccggg ctcgggcatc atgtcgtcgg tgatcggcgg ctcgttccag
     1021 gcctacgacg gcacctcgtt tgcggccccc cacgtcgccg gcgcgatcgc cgcgatccga
     1081 tcgcgtcatc ccagggcctc gctcgacgcg atcgaggccg cgctcaaggt gacagggcca
     1141 cgcgtcagcg accagcggcc cgaaggccag cacgccaagc cgcgcatccg cgtcgatctc
     1201 gcccttcgtc tcctggcggg tgatgccaat gtggcagcga gcaggacgcg tgcggcgccc
     1261 tccggcacga cgaccaccac cccgaagggc aatccggcgc ccgcgccggg gtccgccaca
     1321 ccagcgacac ccggttgcga tgatgtcagc cgacgcgcat ccggcgcact gaccggcggc
     1381 accggcgacg cggcacgatg caattgacct gaattccgat tgaaggagca tgccacatga
     1441 agttccgcga aatccagacg gcaaaaggct ggacgaccgg ccgcgccatc ggcgcagcag
     1501 cgtcggcggc cattctcgcc gcactcgccg gatccgcggt cgaagcccag acgagcgacc
     1561 tcggccgcaa gtcagggacg gcgaccgccc gcctcatcga gtcgcagctc gccgcgcgcg
     1621 gctccgctcg cgtcatcatc atgcacgatg cgccggccgg tcgggccgat agcgccgatg
     1681 cttcgcgagc cctgctggcg cgcgccaccg acacacttct ctccggagtt ccggcgagcg
     1741 atgcacggcg cgccgaatgg tcggtcaatg cggcgagcct gatgccggtg ttcgccgtca
     1801 ccatgaaccg ggccgagctc aatgcggttg cgcggagcaa gaacgtggtg cgcatctggc
     1861 ccgaccagcg caaccgcttt gcgctcgacg gcagcatgcc ctcgatcggc ctgccgaacc
     1921 cgctgccgcc tgccgttgcg ggtgccgacg gcaagggcgc caccattgcc gtcctcgact
     1981 cgggcgtcga ggccggccac cccttcttca acggacgcgt ggtcgccgag gcctgcttct
     2041 cggcgaacgt gaagggcgag agcgcctcgc tctgtccgaa caaggcgaac acgcagaccg
     2101 gggcgggatc gtccgccccc tgcaaggcgc cgttcgaggg ctgcgagcat ggcacgcacg
     2161 tcgccggcat cgcagccggc aaacggagcg gcaacaccgg cccggccggc ggcgtggcac
     2221 ccggtgccaa catcctcgcc gtcaacatct tctcgaccaa ctgcgacgcg ggccagagct
     2281 gccgcgagat cagctcgtgg gattcggatg gcatcgccgc gctaaactgg gtcctcgagc
     2341 agcacgcgcg catcccgggt ggtgtcgcgg ccgtcaacat gagcatcggg gccggcgagt
     2401 tcgcgggcgc ctgtgacgac cggccataca aggtcgcgat cgacgcgctc agggcggcag
     2461 gcattgccgt ggtcgtcgcc tcgggcaact cgcgcttcaa gaacgccgcc ggctcgcccg
     2521 gctgcgtgtc gagcgcggtg accgtcggtt cgattaccaa ggccggccgc atctccgagt
     2581 tctccaacat gtcgccgatc gtcgacctga tggcaccggg tacagccatc atgtcgtcgg
     2641 tgctcaacgg ccgcttcgag agcatggatg gcacctcgat ggcgacgccg cacgtgacgg
     2701 gcgcgttcgc ggtgctcaag gctcggttcc ctcgcgcgag cgtcgccgag ctcgaggagg
     2761 cgctgcgcgc gaccgggccg gcgatccgcg acgaccgccc gggcggcacg cattcgcggc
     2821 ccggcatcaa ggtcgatctc gccattgcgc acctcgccaa gacgcatacg ggcgtcgccg
     2881 cgacgccgcc gccagcaacc agcaccaccc cgccgccgcc ggtcgccaag ggaccgacac
     2941 cgccaaatcc atgcgatacc acctcggcgg cgagcagcgc gctcaccggc ggcagtgccg
     3001 acaaggccgg ctgcaagtag tccggtcgag ccatcccgga atgcatgagg ggccctcatc
     3061 ggggcccctt tttgctcggc ccgctcgcgg tctatgatgc tctcgacgag ttcgtgacgg
     3121 agccgtgagg agacgccgat gaacgccaag cccaacaccg atctgaagcg ctcgcccgat
     3181 cgggcggcaa aagcgaaggt cgtcgccgca gcggaggccg ccgagacgaa cctgccgatc
     3241 gccgaggcgc tcgccgcctt cacgatgcag acgcgtgccg aggacatccc ggccgaggtc
     3301 cgcacgcgcg cggtccacca catgctggac gcggccggca tcgcggtcgc cgcctcgcgc
     3361 tacgacttct cgcaccgtat gctgactgga atccgtggcc tcgcaggcga gggaccggtg
     3421 cccgtcatcg gcatgccggc gcgccttccc gcgcgtgacg ccgccatgat gaacggcttc
     3481 ctctgccatg gcctcgactt cgacgacacg catgtcgcgg ctatcgtgca tccgacggcg
     3541 agcatcctgc cgacggtgct ttcggccggt gccatgacgg gtgcctcggg tcgcgacatg
     3601 gtggcggcct tcgtcatcgg cgtcgagacg gcggtgcgca tcggcatggt cgcaaagagc
     3661 ggcttccatc aggtgggctt ccatccgacc ggcatggtcg gcgtgttcgg ctgcacgctc
     3721 gcggccggcc ggcttatggg cctcaacgac gcggagctgc gcaatgcgca aggcatcgcg
     3781 ctttccatgg cctcgggctc gctcgagttc gtcgccgacg gcgcctggaa caagcgcctc
     3841 catccgggct gggcggcgca tgccggcatc acggcggcag ccctcgcgcg tgagggcttc
     3901 atcggcgcga ccaagccata cgatggccga ttcggcctct tcccatccta tctcggcaag
     3961 gagagcgacc gggccgacta tccgatcgcg acccgcgggc tcggtgacac gtgggagctg
     4021 atggcgacgg cgatcaagcc gtatccggcc tgccatttca cgcacgcctg catcgattcc
     4081 gcactcgcgc tccgcccgca ggtcggcgac tggcgccgca tcaagtcgat cgaaacgctt
     4141 gtgccggaag gcgtgttcaa gaccgtgtgc gagccggagg cgggcaagtt gaagcctgca
     4201 aacagctatg acgcgcagtt ctcggtgcag tacctgacgg ccgtggcact cgtgcgtggc
     4261 aaattcggtc tcgccgagat cgagcccgag gcgatcagca attcggacgt gctcgcgctg
     4321 acgtcgaagg tgaagtacgg cgcctatcag gacggcgtgt tcccgaaact ctatcacggc
     4381 gaggtcgtga ttactctgga tgatggccgc acgctccgcc atcgcgagca tgtcaatcgc
     4441 ggcgccgccg aacgtccgct caccaacggc gagatcgtcg agaagttcaa cggcaatgcg
     4501 gcccgcggct tcgatcgtga caccgcgcgg cgcatggagg ccgccatgct cggcctcgag
     4561 aaggcttcga acgtcgatgg cgtgttcgcg gccttctcac cgcggctccg atgatcgacc
     4621 acgtctcgct ctccgtgagc gacatggcgc ggagcacacg cttctaccgg gcggcgctcg
     4681 agccgctcgg ctatcgctgt ctcaccgaga gctccgagcg agcggcgttc ggcaagcgct
     4741 atccggaact ctggctcaat gcccggcccg ggatgcgccc gatcgaggcg gcaaccggcg
     4801 cccacatttg cctccgggcg cggacgaagt ccgagatcga ggcgttccac gcgggagccc
     4861 tcgcggccgg cggcacctgc gacggcccgc ccggcatgcg aaaggcattg atggtcgatt
     4921 acttcgcggc cttcgtgcgc gaccccgatg gcaaccgcat cgaagcgatg acggtgccgg
     4981 cggcgggcgc gtaacgctga ccgggtcagc tgagaacgac ctcacgtgcg ggtgcgtccg
     5041 ttgccgctcc cgcattgtcg tggagatggc attccacggc ggcctgtccg acgagggacc
     5101 ggcgcggcgc acgcaccgag cagtccggca tcttgtagcc gcagcggggg tggaagctgc
     5161 agcccggcgg cacgtcgagc ggattcggat atgcggcgcc gaggttcgta tcggggatgc
     5221 cgcgaccggg ctccggcgtc agcaccgact tcaagagcgc ttccgtgtat ggatgcttcg
     5281 ggtgcgcgaa gagtgcgtcc gtcggcgcct cctcgacgat gcggccgagg tacatgacgg
     5341 cgacgcggct cgccatgtgc tcgacgacgg cgaggtt
//
LOCUS       DAORNH010000313         5345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11404_length_5345_cov_2.479395, whole genome shotgun sequence.
ACCESSION   DAORNH010000313 DAORNH010000000
VERSION     DAORNH010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11404_length_5345_cov_2.479395"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK264_21970"
     CDS             <1..183
                     /locus_tag="PK264_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78571.1"
                     /translation="LGLPPAFVLSQDQTLKLKVEFWHGLEESLQGSLCNGHLHTDALT
                     RALLPEVRIMVMAETQ"
     gene            1014..>1581
                     /locus_tag="PK264_21975"
     CDS             1014..>1581
                     /locus_tag="PK264_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HRD78572.1"
                     /translation="MAAIVGGIAIAAATLSSLDSRDAEGGLIPAFTRIADDTTFSTAV
                     ARPSDGLNWALPKSDRLNVITGAASTRFIIHEPVRQVRKGNEYLHNRPYARVLARLAV
                     AQPMPATEIPAFDPLKLYAGDSAATDPATAAASDPKSVAVEVVELLGGILPLEDGQEL
                     NNGEVGEIIARIEANERASSPLRPSLVPE"
     assembly_gap    1582..1600
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1601..>2065
                     /locus_tag="PK264_21980"
     CDS             <1601..>2065
                     /locus_tag="PK264_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78573.1"
                     /translation="GARPPGTSPTAPAPAGIDKAEPATTVIAKASGDSDDGAEDDDGN
                     TLRSFKALRGETLSRVLVRAGIESSLTRTIVEAARAILKDDDLLGGDEVQIAATPLST
                     APSRLSPARLLVYSSAGDHRLSVVRGVNGDYVASADPLGRVDTGEPRPKSRI"
     assembly_gap    2066..2091
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2092..2296)
                     /locus_tag="PK264_21985"
     CDS             complement(<2092..2296)
                     /locus_tag="PK264_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78574.1"
                     /translation="MHDTIDGHLGGEQVAEGVPRAALVANTEEQLDGIAAAGAAPEID
                     VIGMDADDAIDRIDWYRLGKQGVE"
     gene            2291..2887
                     /locus_tag="PK264_21990"
     CDS             2291..2887
                     /locus_tag="PK264_21990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRD78575.1"
                     /translation="MHRYWRFQLPDGTIDYFDENGQNSRKFLIKVPIKMIDPRLTSGY
                     GFRTHPTLNVRRMHTGVDWAAPSGTPIIAAGSGVIEELGWKGHFSYGHYIRIRHANGY
                     QTAYGHLSRYAPGLREGSRVTQGQLIGFVGSTGQSTAPHLHYEVIVGDNFVDPMSLKS
                     PQARILAGADAIAFRKERRRIEELSRRPPVRIEHLDAR"
     gene            complement(2924..3673)
                     /locus_tag="PK264_21995"
     CDS             complement(2924..3673)
                     /locus_tag="PK264_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78576.1"
                     /translation="MAGRMSGKTAFVTAAAAGIGRASALAFAREGARVIATDVDEKGL
                     VSISSGGVETHRLDMRDTGAVRALAAKVGSVDVLLNAAGFVHHGSALECSDADWDFSF
                     DLNVKSMHRAIQAFLPGMLERHKGSIVNIASGAGPSKAVANRYVYTATKAAVVGLTKA
                     VAFDFVGKGIRCNCICPGTIETPSLGDRIKSLGDKVGGTETARRMFVERQPMGRLGTA
                     EEVAHIAVYLGSDESAFTTGSSFFVDGGFSL"
     gene            3677..>3873
                     /locus_tag="PK264_22000"
     CDS             3677..>3873
                     /locus_tag="PK264_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78577.1"
                     /translation="MLLDLLDVMSIEFMTGRLEVKRRSPGCGKRPSMAIFGSPIGPAS
                     AVEEAAGSASPDRLAGMSSAVA"
     assembly_gap    3874..4006
                     /estimated_length=133
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4007..>4264)
                     /locus_tag="PK264_22005"
     CDS             complement(<4007..>4264)
                     /locus_tag="PK264_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78578.1"
                     /translation="KYRQGIAFLVFVNNDFGISAAHPVDEARLPSPSSKLWHGQAAGF
                     RALHSACGRTLFVSFKETVMSIRSIGVAAGLVLALAVTVAPA"
     assembly_gap    4265..4415
                     /estimated_length=151
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4416..4796)
                     /gene="rimM"
                     /locus_tag="PK264_22010"
     CDS             complement(<4416..4796)
                     /gene="rimM"
                     /locus_tag="PK264_22010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRD78579.1"
                     /translation="MSSASRVLLGRIGRAHGLRGEVAVESYTRDPRDIASYGPLSDDD
                     GNKRLVLTIVGTRENSVIARIEGVTDRTAAEQLRGTALWIARSALPPPEPGAYYHVDL
                     IGLMATLPDGAELGRVAAVENFGAG"
BASE COUNT          921 a         1627 c         1583 g          885 t
ORIGIN      
        1 ttaggcctgc cgccagcgtt cgttctgagc caggatcaaa ctctcaagtt gaaagtcgaa
       61 ttctggcacg gcttggagga gtctctccaa ggttcactgt gtaacgggca ccttcacacc
      121 gatgcgctca ctcgagcatt gctgcccgag gtgcgcatca tggtgatggc tgaaacgcag
      181 tgaacgccag agtctcgttg attgcacgag ccgttgccga cccggcaatc cgccaggact
      241 ccgccgcctg cgtttccctt tcttcgatct acttgtcaaa gagcaacatc aaaagacgcc
      301 ttctgcggca agcccaacca ggcctaccgt tcgggaacaa gctttctctt cgggctgccc
      361 cgttgagtga gctattgttc gagagggtcg tctatcgacg gtgaggcgtc cgccgcgtgt
      421 ccgcagcgcg ctcaacacgg cggaattaat cctagacgct gcccgatgtc aacccccatc
      481 gaacacgatt ttgaaggatc cctcggtgaa atcgtataac tgtcagaaat aacacatgat
      541 tttatgttgt ccacaagccc ggcaccaggg ccgccactgc cgattcgcga cagtctcgtg
      601 tcgtccacaa tataggcctc cgtcgccggg acgccccttt tttgccggac ggatcacgca
      661 catactcgtc ggcggaaaaa aaaagtgcga tcctgtcgtt gagcaagtgc gggatggggg
      721 cctgccgccg agtccgcggc ccaagcgcct gattcggaag ggaaagcggg ccatccgata
      781 tcgggggcgc gtggcgccgc agcggacagg gcgcgaccgg agtagggagg agaatgatag
      841 aagctcgtcg ccatgctcca ttcggccgtg cacggcagca ctctgcggcg gccaacggca
      901 ccgtgcggcc cgcgctcggt cgcatgggca cacgccggca gcggcaacta cctgacctat
      961 acgccgtcga gccgcgcgct catgggaaat ttcgctggct gatcagcacc ttcttggcag
     1021 cgatcgtcgg tgggatcgcg attgccgcgg cgacgctcag ctctctcgac agtcgcgacg
     1081 ccgagggtgg gctcatcccg gctttcaccc gcatcgccga cgacacgact ttttcgacgg
     1141 ctgtcgcccg tccctctgac ggcctcaatt gggccttgcc caaatcggat cgcctaaacg
     1201 tcattacggg cgcggcctcg acccggttca tcattcacga gccggtccgg caggttcgca
     1261 agggcaacga atatctgcac aatcgtcctt acgccagagt gctcgcacga cttgcggtcg
     1321 cgcagcccat gcctgccacg gagatcccgg cgttcgatcc gttgaagctc tacgccggtg
     1381 acagtgccgc caccgacccg gcgacagccg cggcgagcga cccaaaatcg gtggccgtcg
     1441 aggtggtcga gctgcttgga ggcattctcc cgctcgagga tgggcaggaa ctcaacaatg
     1501 gcgaggtcgg cgagatcatc gcccgcatcg aggcgaacga gcgagcgtcg agcccccttc
     1561 ggccctcact cgtacccgag tnnnnnnnnn nnnnnnnnnn ccggcgcccg accgcctggc
     1621 acgtcgccga cggcgcccgc gccggcaggc atcgacaagg ccgagccggc aaccactgtg
     1681 atcgccaagg catcgggcga ttccgacgac ggggccgaag acgacgatgg caacacgctc
     1741 cgctctttca aggcattgcg cggcgagacg ctcagccggg tactggtgag agcgggcatc
     1801 gagagttccc tgacgcgcac gatcgtcgag gctgcaagag ccatcttgaa ggatgacgac
     1861 ctgcttggcg gcgacgaggt gcagatcgcg gcgacgcccc tctcgaccgc gccgagccgg
     1921 ctgtctccgg cccgcttgct tgtctactcc agcgccggcg atcaccgctt gtcggtcgtg
     1981 cggggcgtga acggcgacta cgttgcctca gcagacccac tcggccgggt cgacacgggt
     2041 gagccgaggc caaagtcacg gatcgnnnnn nnnnnnnnnn nnnnnnnnnn ncttccacgc
     2101 cttgcttgcc cagtcggtac caatcgatac gatcgatcgc atcatccgcg tccatgccta
     2161 tgacgtcgat ttccggcgcc gcccccgcgg cagcgatgcc atcgagttgt tcttcggtgt
     2221 tcgcgacgag cgcggcccgc ggcacgccct cggcgacctg ctcgccaccc aggtgaccgt
     2281 cgatggtgtc gtgcatcgct actggcgctt ccagctgccg gatggaacca tcgactactt
     2341 cgatgagaac ggccagaact cgcggaaatt cctcatcaaa gtgccgataa agatgatcga
     2401 tccacgtctc acgtccggtt acggcttccg cacacatccg acactgaatg tccgccgtat
     2461 gcataccggt gtcgactggg cagcgccatc cgggaccccg atcattgccg ccggcagcgg
     2521 cgtgatcgag gaactcggct ggaagggcca tttcagctat ggccactata tccgcatccg
     2581 ccacgccaac ggctatcaga cggcatacgg tcacctctct cgctatgccc ccggcctgcg
     2641 cgaaggctca cgcgtgacgc aaggccagct catcggcttc gtcggctcga ccggccagtc
     2701 gacggcgcca catctccact atgaggtgat cgtcggcgac aacttcgtcg acccgatgag
     2761 cctgaaatcg ccgcaagccc gaatcctggc tggcgccgat gccattgcct ttcgcaagga
     2821 gcggcggcgg atcgaggaac tgtcgcggcg tccgcccgtc aggatcgagc atctcgacgc
     2881 gcggtgagac ctattccggg gcgccgaccg ggtccgccgg acgctacagc gaaaagccgc
     2941 catcgacgaa gaaggaggag ccggtcgtga aggcgctctc gtcggaccca agatagaccg
     3001 cgatgtgcgc gacttcctcg gccgtaccga gccggcccat gggctggcgc tccacgaaca
     3061 ttcggcgtgc cgtctcagtg cctccgactt tgtcgccgag cgacttgatg cggtcgccga
     3121 gcgacggggt ctcgatagtg cccgggcaga tgcagttgca gcggatgccc ttgccgacga
     3181 aatcgaaggc gacggccttg gtcaggccaa cgacggcggc cttggtggcg gtgtagacgt
     3241 agcggttggc gacggctttc gacgggccag cgccagaggc gatgttgacg atcgagccct
     3301 tgtgacgctc gagcatgcca ggcagaaacg cctggatcgc gcgatgcatc gatttcacgt
     3361 tgagatcgaa ggagaaatcc cagtccgcat ccgagcactc gagcgccgag ccgtgatgga
     3421 cgaatccggc agcgttgagg agaacgtcga ccgagccgac ctttgccgcg agcgcacgga
     3481 ccgcgcccgt atccctcatg tcgagtcgat gggtctcgac gccgcccgag ctgatgctca
     3541 caagtccctt ctcgtctacg tcagtggcga tgacacgcgc gccctcgcgg gcaaaggcga
     3601 gcgcagacgc tcgcccgatt cctgccgccg ctgccgtcac gaatgccgtc ttgccgctca
     3661 tgcgtccggc catggcatgc tcctcgacct acttgatgtg atgagtatcg agtttatgac
     3721 aggacggctg gaagtcaaaa ggcgctcgcc gggatgcggc aagcgccctt cgatggcgat
     3781 ttttgggagt cctatcggac ctgccagcgc ggtggaagag gctgcgggtt cagcatcacc
     3841 ggaccgcctg gccggaatgt ccagcgcggt ggcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgg gggcaactgt
     4021 gacggcaaga gccagaacca gcccggcggc gacaccgatt gatctgatag acatgacggt
     4081 ctccttgaat gagacgaaaa gtgttcgacc gcatgcagaa tgcagcgctc ggaagccagc
     4141 ggcctggccg tgccagagct tcgaggaggg cgaaggcagc ctggcttcgt cgacgggatg
     4201 ggctgccgag atgccgaaat cgttgttcac aaaaaccaaa aaagcaattc cttgccgata
     4261 cttannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcccg ccccgaaatt ttcgaccgcg
     4441 gcgacccggc ccaactctgc gccgtcgggc agcgttgcca tgagcccgat gagatcgaca
     4501 tgatagtagg cgccgggctc aggcggcggc agcgccgagc gcgcgatcca gagcgcagtg
     4561 cctcgcagct gctctgcagc cgtcctgtcg gttacgccct cgatgcgggc aatgacgctg
     4621 ttctcccgcg tccccacgat cgtgagcacg agcctcttgt tgccgtcgtc gtcgcttagg
     4681 ggcccatagc tcgcgatgtc gcgcggatcg cgggtgtagc tctcgacggc gacctcgccc
     4741 cggagaccat gtgcgcgacc gatgcgtccg agcaggacgc ggctcgcaga cgacatgtgg
     4801 ttccctcgtt cgcaaaatgg cgcctcgcat gtggagcggg ctcctgttcg gcagtcctcg
     4861 ctgaccgcca aacctgcggc aagcggcacg tcgccgctga cccggatgtg gtgtcagccg
     4921 gccccagggc tcatgttgca gccggcaggg taactaccta gggcacgccg gccgcagcca
     4981 aagttaaagt gggttcggcc gtaaggctcc tgtcgctagg ccccgtctct ttcacccacg
     5041 tcggagatcg ggcacgagcg gcaacggcag gcagcaggga gccttgcggc catgggggag
     5101 attatccccc gacaaagcta accgccctcc ggaaggaact atttcttccc ggcgggtggt
     5161 ttggcgcgtc tgagggcagg ccttcgtggt tgcttgcagc cgatgctgcc gggtacttca
     5221 acatgcgcga cgcgcttcgg gccgagtata tcttctgtgt gctcgcctgc ggctggaaca
     5281 gagcgggcga ccgtgaggcg ctgacgatcg agcgacaccg ggatctgcac ggtgcggacg
     5341 ggtcg
//
LOCUS       DAORNH010000314         5332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11439_length_5332_cov_2.513170, whole genome shotgun sequence.
ACCESSION   DAORNH010000314 DAORNH010000000
VERSION     DAORNH010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_5332_cov_2.513170"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PK264_22015"
     CDS             <1..449
                     /locus_tag="PK264_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78580.1"
                     /translation="PTTALDVSVQAQILNLMRDLQDRFGLTYLFISHNLAVVRHMATR
                     IGVMYLGRLVEVAPAKELFQAPRHPYTRLLLDAVPDLAMSGRPRKAIEGEIPNPISPP
                     PGCAFHPRCPDAFERCRVEVPRPLTVRTRMTACHAIEEGRVAGGGA"
     gene            449..1423
                     /locus_tag="PK264_22020"
     CDS             449..1423
                     /locus_tag="PK264_22020"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRD78581.1"
                     /translation="MKLLAVLATLVVGGLIALSAYFFLGSRGHGLAWAVGWLTLTVIV
                     IASGAARLSWPASAFWVAALAWGVWWATLTPSNNRAWASDVANLPSGALSGEIVTLTN
                     VRNFSWKSPTEAVETWETRSYDLAKLTELDLFASYWSGETMAHLILSFGFTDGQRLAI
                     SIETRREKSETWSAFAGFFKSYELIYIAADERDVVKLRSNVRGEEVRLYRLKSTPEMR
                     KRLFVQYINEMNSLVDRPRFYHTIWTNCTTQIAGIARSIGRNVPLDWRIIASGYVPNL
                     LYDLELLDKRLPFPDLRRAANIVPRAKAADADPQFSELIRAGVPSPAR"
     gene            complement(1477..2250)
                     /locus_tag="PK264_22025"
                     /pseudo
     CDS             complement(1477..2250)
                     /locus_tag="PK264_22025"
                     /inference="COORDINATES: protein motif:HMM:NF017487.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
     gene            2503..2902
                     /locus_tag="PK264_22030"
                     /pseudo
     CDS             2503..2902
                     /locus_tag="PK264_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892254.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     assembly_gap    2903..2983
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2984..3356
                     /locus_tag="PK264_22035"
     CDS             <2984..3356
                     /locus_tag="PK264_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRD78582.1"
                     /translation="QSAAVVIGGDPVKGLDFIDVLEMFLADPDTHSIIMIGEIGGSAE
                     EDAAEFVKRERARGRSKPMAGFIAGATAPPGRRMGHAGAIISGGKGKAEDKFEAMRSA
                     GIAIADSPAALGTTLVKAIKG"
     gene            3418..4005
                     /locus_tag="PK264_22040"
     CDS             3418..4005
                     /locus_tag="PK264_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78583.1"
                     /translation="MIIHLNGWPGAGKRTIGEALARRLGARFVHNHLLHDVARVSVGQ
                     GGRDNDAYWSTYERVRSAAYDALALRPRCETFVMTNALVKDDPRETEAWGHVVALAMR
                     RDVPLVPVVLDIALEENCRRVASPDRVGRKLSDPTLIREFRRTLAIQHPDVAELIVLD
                     VTGLSPEMAALTIEEGIAAVRPALKTATLDHLRIR"
     gene            4045..>4724
                     /locus_tag="PK264_22045"
     CDS             4045..>4724
                     /locus_tag="PK264_22045"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRD78584.1"
                     /translation="MTLFIDILTKVTLPIIALFALGYLLQPRLKLDVATLNRVQVYVV
                     MPAFLVHFLSSGKQPLSIIWPVAYFGIVQFMILIPLGWLMVILFRRRRTLGPMMGLGT
                     AYANVGFFGIPVTQLAFGSEYLIYQSVLTALMAILVCTLGVWLMAPSGTSRISKLKTA
                     FETPLIPSVAIGLALRGFEIELPHVLTQPMQFLGSIFTPLALYTLGAQIAAAKSIRFE
                     FVPQTLIL"
     assembly_gap    4725..4754
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4755..4951
                     /locus_tag="PK264_22050"
     CDS             <4755..4951
                     /locus_tag="PK264_22050"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRD78585.1"
                     /translation="IAMGLPRDVTNVIVVASATPVGVLIAVFAAEYKTESEFISTAVV
                     VSTMLSPIFVTGWILAVRLL"
BASE COUNT          910 a         1747 c         1687 g          877 t
ORIGIN      
        1 agccgacgac cgcgctcgac gtctcggtgc aggcgcagat cctcaacctg atgcgcgacc
       61 tgcaggaccg cttcgggctc acctacctct tcatctcgca caacctcgcc gtcgtccggc
      121 atatggcgac acggatcggc gtcatgtatc tcggccggct ggttgaagtg gccccggcga
      181 aggagctctt tcaagcaccg cgtcatccct atacgcggct cctccttgat gctgtgccgg
      241 acctcgccat gtcggggcgg ccgaggaagg cgatcgaggg cgagatcccg aacccgatca
      301 gcccgccgcc cggctgtgct tttcatccgc gctgccctga cgccttcgag cgctgtcgcg
      361 tggaggtgcc gcggccgctg acggtccgga cgcggatgac ggcgtgtcat gctatcgagg
      421 agggacgagt tgctggggga ggggcgtgat gaagctactg gccgtgctcg cgacgctggt
      481 cgtcggaggg ctgattgcgc tatcggccta cttttttctc ggcagtcgtg gccacgggct
      541 cgcctgggcg gtcggctggc tcaccctgac ggtgatcgtg attgcctccg gtgccgcgcg
      601 cctaagctgg ccggcgtccg cgttctgggt cgcagcgctt gcctggggcg tctggtgggc
      661 gacgcttacg ccgtccaaca accgggcctg ggcgagcgat gtcgccaacc tgccgtcggg
      721 ggcgctgagc ggcgagatcg tgacactgac gaatgtccgc aatttcagct ggaaatcgcc
      781 gaccgaggcc gtcgagacct gggagacgcg ctcctacgat ctcgccaagc tcactgagct
      841 cgatctcttc gcctcgtact ggtccggcga gaccatggcg cacctgattc tctcgttcgg
      901 cttcacggac ggccagcgcc tcgccatctc gatcgagacg cggcgcgaga agtccgagac
      961 gtggtcggcg ttcgccggct tcttcaagtc ctatgagctc atctacatcg ccgccgacga
     1021 gcgggacgtc gtcaagctgc gctccaacgt gcgcggcgag gaggtgcggc tctaccggct
     1081 gaagtcgacg cccgagatga ggaagcggct tttcgttcaa tatatcaatg agatgaactc
     1141 gctcgtcgac cggccgcgct tctatcacac gatctggacc aactgcacga cgcagatcgc
     1201 cggcatcgca cggtccatcg gccgaaatgt accgctcgac tggcgcatca tcgcctcggg
     1261 ctacgtcccg aacctcctct atgatctcga gttgctcgac aagcggttac ccttcccgga
     1321 cttgcggcgc gcggccaaca tcgtgccacg ggcgaaggcc gcggatgccg atccacagtt
     1381 ctcggagctg atccgggccg gtgtgccgtc gccggcgcgc tgatcgacca cttccggcac
     1441 gtgatggacg ctcgccacca gcacgaagct gacgatcatc aggagatacc aggagccgag
     1501 cttgccgagc gtcaccggcc ggaagcccga catctggtga gggtagagcc agaccgccga
     1561 ccagctgccg atgttctcgg ccacccagat cagcagcgcg acgagcacga agccggaaag
     1621 gaggggcagg cgccgctcgc ggcggtcgat cgtcatggca atcgcggtgc cgcggaacat
     1681 gacggccgcg gccgcgaaga gcagtaagcg cgcgtcccag acaaaatggt gcgtgaagaa
     1741 gttcgcgtag ataaggatcg cgagcgcgat cggagcgcgt ctcgccggat aaccctccag
     1801 ccggacagca aacagccgcc agcagcgcgc cagatatgag ccgacggcgg aatagagaaa
     1861 gcctgagaac agcggaacgc ccgcaagccg gagccagcag gcctccgggt agacccagga
     1921 cccgcgcgcg gtcttgaaga gctccatcac tgtcgcgagc acgtgaaagg ccaggatgac
     1981 cttggcctcc tcgatccgct cgtagccaag ccggatgagg acgatctgga tcgccacggc
     2041 atagaccagc aggaggtcgt agcgcgcgat gggcaacgcg gtcggatcca ggcggcgcgt
     2101 tgcaacgatc gccgcgagca gaaggacggc gaaggccgcg gcggcgatct gcttgcgcag
     2161 gaaggcgagt gcctcctgag cgatgtccac atgcggcctg gacgaagcgg cggtcggggg
     2221 cggcgtcgac gaaacgagcg ggttggtcat cgcccgaaac tgctggcagg aagcgccgcg
     2281 cgcccctgga cagtctagcc ttgacccggc acggcccgga caacgccgcc gcggtggtgc
     2341 gtcctagtgc ggccggaggc ccggatgcag cacagcccgg gctccgacga tcgccctact
     2401 tgatcaggcg gccaatctgt cccacaaggc gtcgatcagc cgaagggaca ctcgcgcatg
     2461 gccatcctcg tcgacaagag cacccgcgtc atctgccagg gcatcaccgg cagccagggg
     2521 acgttccata cgcagcaggc gctggcctac ggaacgcaga tcgtcggcgg cgtcacgccc
     2581 ggaaagggcg gcaccaagca ccccgacgcg aagctcgcgg gtgtgccgat cttcgacagc
     2641 gtcgcggacg cgaagaagaa gacaggtgcc acggcaacct cgatctacgt ccctccgccc
     2701 tacgccgcgg atgccatcct cgaggcgatc gacgccgaga tcccgctcat cgtgtgcata
     2761 accgaaggca ttccggtgat ggacatgatc aaggtgaagc gggcgctgac cggctcctgg
     2821 tcgcgtctca tcggaccgaa ctgcccgggc gtgctgacgc cgaaccagtg caagatcggc
     2881 atcatgacgg gcaacatctt cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccagtcg gcggccgtcg
     3001 tcatcggcgg cgatcctgtg aaggggctcg acttcatcga cgtcctcgag atgttcctcg
     3061 ccgatcccga cacgcactcg atcatcatga tcggcgagat tggcggatcg gcggaagagg
     3121 acgcagccga attcgtcaag cgcgagcgcg cacgcggccg ctcgaagccg atggcgggct
     3181 tcatcgcggg cgcaacggca cctccgggaa ggcgcatggg acacgccggc gccatcatct
     3241 ctggtggcaa gggcaaggcc gaggacaagt tcgaggcgat gcgctcggcc ggaatcgcca
     3301 tcgccgacag tccggcggcg ctcggcacga cgctcgtcaa ggcgatcaag ggctgatctc
     3361 gccgcggtgc cactgcgcac gaccgatgca ggtcgcaccg ctgcgatgga ggcgccgatg
     3421 atcatccatc tgaacggctg gccgggagcc ggcaagcgca cgatcggcga ggccctggcg
     3481 cgacggctcg gcgctcgctt cgtccacaat cacctgctgc acgatgtcgc ccgcgtttcg
     3541 gtcgggcagg gcggccgcga caatgacgcc tattggtcga cctacgagcg ggtccgctcc
     3601 gccgcctacg atgcgctggc actccgtccg cggtgcgaga ccttcgtcat gaccaatgcg
     3661 ctcgtcaagg acgacccgcg agagaccgag gcctgggggc atgtcgtggc gctggccatg
     3721 cgtcgcgatg tgccgctcgt tccggtcgtg ctggacatcg cgctcgagga gaattgccgc
     3781 cgcgtcgcga gccccgatcg cgtaggccgc aagctctctg atcccacgct cattcgcgag
     3841 tttcgtcgca ccctcgcaat ccagcacccc gacgttgccg agctgatcgt cctcgacgtg
     3901 acggggctct ctccggagat ggcggcactg accatcgaag aaggaattgc tgccgtgcgc
     3961 cccgcattga aaacggcgac gctcgatcac ctccggatca ggtaacgagg caggacgtcc
     4021 ccgccggaac agagcgccgc tgcgatgacc ctttttatcg acatcttgac caaggtgacg
     4081 ctgccgatca tcgcgctgtt cgcgctcggc tacctgctgc agccgcgtct gaagctcgac
     4141 gtggcgacgc tcaatcgcgt gcaggtctac gtcgtgatgc cggccttcct cgtgcacttc
     4201 ctgtcgtcgg gaaagcagcc gctgtcgatc atctggccgg tcgcctactt cggcatcgtc
     4261 cagttcatga tcctgatccc gctcggctgg ctcatggtga tcctgttccg ccggcggcgc
     4321 acgctcgggc cgatgatggg gctcggcaca gcctacgcca acgtcggctt cttcggcatt
     4381 ccggtgacgc aactcgcctt cggctcggaa tacctcatct accagtccgt gctgacggcg
     4441 ctgatggcga tcctcgtgtg cacgctcggc gtctggctga tggcgccctc cggcaccagt
     4501 cgtatcagca agctcaagac ggccttcgag acgccgctca ttccctccgt cgccattggg
     4561 ctcgcactgc gcggtttcga gatcgagctg cctcacgtgc tcacgcagcc catgcagttc
     4621 ctcggctcga tcttcacgcc gctcgcgctc tatacgctcg gcgcgcagat cgcggcggca
     4681 aagtcgatcc ggttcgagtt cgtgccgcag acgctgatcc tgttnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnngcatcg ccatggggct gccgcgggac gtgacgaacg tgatcgtggt
     4801 cgcctcggcg acgccggtcg gcgtgctgat cgcggtgttc gcggccgagt acaagacaga
     4861 aagcgagttc atatcgaccg cagtcgtcgt ctcgaccatg ttgagcccga tcttcgtgac
     4921 aggctggata ctggcggtcc gattgctttg aggcggggca cggaaggctc ctccgagtga
     4981 gccgtcgcat catgcctccg tggtccgacc ttgtggcccg gcgacgcgaa tgtcctcggc
     5041 aagccccagc accaaagcac cccagcacca aagcacccca gcaccaaagt acccccagca
     5101 cggtatgctg aagccatgcg ctcgtccttg gtttgctcta tctcttgact cgccccgtcc
     5161 ggccccgcta cctgcaccgg cggttgcgcc gcgaaacgtg ctggtctggc cgaggttcta
     5221 accgacctcg catcggacca gcctgacgcc gtggtgaatt ggcgcggacg ctcgcaagcg
     5281 gccgacaacg cacctccgcg ggtgtagctc aatggtagag caacagcctt cc
//
LOCUS       DAORNH010000315         5325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11463_length_5325_cov_3.047059, whole genome shotgun sequence.
ACCESSION   DAORNH010000315 DAORNH010000000
VERSION     DAORNH010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11463_length_5325_cov_3.047059"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PK264_22055"
     CDS             <1..1336
                     /locus_tag="PK264_22055"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRD78586.1"
                     /translation="ATDQGKTGGLVGAAVLAKARGLAIEAVGLPTMRPYTSPVSWGAF
                     AGREVGRHFHAVRRTPLDDWNRRNGAVFMDAGLWARAAYFPKAGEGAWDAILREARTV
                     RQAVGICDVSTLGKIDVQGRDAATFLDRIYTNTFSTLPVGRARYGLMLREDGIVFDDG
                     TTSRLAPDHFLVTTTTAKAAEVLEHMEHYAQVVWPELDVQICTVTDQWGQMSIAGPRS
                     RDVLAACVEGAALDNASLPFMGVVEARIAGVAVTLFRISFSGELAYEVAAPAGATEAV
                     WSAILTAGAPHGIAPYGVEALAVLRVEKGHVAGAEINGQTTAGDLGLARMLKKNGDFV
                     GRVNAGRPGLVAADRPRLVGIQSLDTDFARRLRAGAQIVESSGSKASLGWVTSVTRSV
                     ELDRWIGLAMVRGGPERIGETLHAIYPLKGEAPPVKLVSPHHVDPENARVRA"
     gene            1323..1934
                     /locus_tag="PK264_22060"
     CDS             1323..1934
                     /locus_tag="PK264_22060"
                     /inference="COORDINATES: protein motif:HMM:NF016180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HRD78587.1"
                     /translation="MSVPSPTTALDVSPLAPFWRPGRRGADVAAPGITIAEPRYAYAQ
                     LIARRGTGTRLVEGIRSTLGLALPEPCRATRSRDVRALDIGPGNWLIAAPRHADAELQ
                     RMLRSAAGDAGSVISQSHGKVTLAISGPATRDCLAKGCRIDLHPRVFLPGHVAVTPIG
                     PVNAIVAALEPAGSFELIVPSSVAAAFLEWLDTSAAEFGVALA"
     gene            complement(1936..2328)
                     /locus_tag="PK264_22065"
     CDS             complement(1936..2328)
                     /locus_tag="PK264_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78588.1"
                     /translation="MPRTGTGAAASFGGVFAAIKPAFARYAKAGVVLHDEPGKYYIGT
                     HQVRTKDGYRTWFGGAEIRKSYVSVHLIPVYAHPELLAGISPELRKRMQGKSCFNLKA
                     PDAALIGELGKLVEASFRRFEADGLFRE"
     gene            complement(2337..2891)
                     /locus_tag="PK264_22070"
     CDS             complement(2337..2891)
                     /locus_tag="PK264_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78589.1"
                     /translation="MLMPKELTFGRLTYALAGFAIGGIVLALALHSAQTRSSRMPVFG
                     SAAPLVGKVEEVGRRLAAEVREASRIDHDVPYVPGDYPAPLQSATCYAIPIAAYRVEG
                     FDEARALDEVRGVLAQFGLGDLKVVVSREPVLVGGRRYMRVMAGTFKTAEDARARCVA
                     VKGVDAKRWCEPLALAAADYPCET"
     gene            3288..4676
                     /gene="cysS"
                     /locus_tag="PK264_22075"
     CDS             3288..4676
                     /gene="cysS"
                     /locus_tag="PK264_22075"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRD78590.1"
                     /translation="MALALYNTLTRRKEPFTPIDPANVRVYGCGVTVYDRIHIGNGRM
                     MVVFDLLFRVLRHVYGERHVTYARNITDVDDKINARAVEEGVTIRELTQRTTLWMHED
                     VAGLGCMPPSVEPRATDHIEEMKTLCEMLVQRGHAYVAESHVLFHVPSMADYGRLSNR
                     TLEEMQAGARVDVAPYKRDAMDFVLWKPSKSGEPAWPSPCGIAEAGRPGWHIECSAMA
                     GRHLGQVFDIHGGGIDLVFPHHENEIAQSRCAHGTPVMANVWMHNGFLEAEAGEKMSK
                     SLGNFITVRELLDRAPGEAGRLALLASLYRQPLPWSEKGVVEARRTLDHWYELTADAT
                     DGAAVDQAVLDALLDDLNTPAAIAALHALRSDAAKGSKGAAAALKSSARLMGFLQLSP
                     ADWKSWRPVGMTIDEAEIRRLIAARLTARKAKDFKEGDRIRDALAAQGIVLKDGKDPT
                     TGEIVTTWEAKV"
     gene            4698..>5325
                     /gene="mutM"
                     /locus_tag="PK264_22080"
     CDS             4698..>5325
                     /gene="mutM"
                     /locus_tag="PK264_22080"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HRD78591.1"
                     /translation="MPELPEVETVRRGLEPTLVGARIARVEQRRPDLRFPLPDRFAAR
                     LTGCSVSSLERRAKYIIAHLDSGEALILHLGMTGRVLISPVGDAKAIIGDFTHDHGSL
                     AAHDHVVLHLESGATITYNDVRRFGFMDLCAADALADNAHLKGLGIEPLGPELTADWI
                     ASATSGRKSPLKAFLLDQRIIAGLGNIYVCEALFRAGLSPQRRAGTLAA"
BASE COUNT          866 a         1875 c         1741 g          843 t
ORIGIN      
        1 ggcgaccgac cagggcaaga ccggcgggct cgtcggcgcg gcggtgctcg ccaaggcgcg
       61 tggcctcgcc atcgaagcgg tcggactgcc gaccatgcgg ccctacactt cgcccgtttc
      121 ctggggcgca ttcgccggac gcgaggtcgg ccggcacttt catgcggtcc ggcgcacgcc
      181 gctcgacgac tggaaccggc gcaacggcgc ggtcttcatg gatgccggcc tgtgggcgcg
      241 cgccgcctac ttcccgaagg cgggcgaggg cgcgtgggac gcgatcctcc gggaagcgcg
      301 caccgtccgc caggccgtcg gcatctgcga tgtctcgacg ctcggcaaga tcgacgtcca
      361 gggccgggat gcggcgacct ttctcgatcg catctacacg aacaccttct cgacgctccc
      421 cgtcggtcgc gcgcgctatg gcctgatgct gcgcgaggac ggcatcgtct tcgacgacgg
      481 cacgacgtcg cggctggcgc cggatcactt cctcgtcacg acgacgacgg ccaaggccgc
      541 cgaggtgctc gagcacatgg agcactatgc gcaggtcgtc tggcccgagc tcgacgtgca
      601 gatatgcacg gtcaccgatc agtggggcca gatgtcgatc gccggccccc gctcccgcga
      661 cgtgctcgcc gcctgcgtcg agggagccgc gctcgacaat gcgagcctcc cgttcatggg
      721 tgtcgtcgag gcgcggattg ccggcgtagc tgtcacactt ttccgcatca gtttctcggg
      781 cgagcttgcc tacgaggtcg cggcaccagc cggtgccacc gaagcggtct ggtcggcgat
      841 cctgaccgcc ggcgcgccgc acggcatcgc cccctacggc gtcgaggcgc ttgcggtgct
      901 gcgcgtcgag aaggggcatg tggcgggcgc cgagatcaat ggccagacca cggccggcga
      961 tctcggtctc gcccgtatgc tgaaaaagaa cggcgatttc gtcggccgcg tcaacgccgg
     1021 gcggcccggc ctcgtcgcgg cggaccggcc gcgcctcgtc ggcatccaat cgctggacac
     1081 agacttcgcc aggcgtctgc gcgccggcgc ccagatcgtc gagagcagcg gctcgaaggc
     1141 aagcctcggc tgggtgacga gcgtcacccg ctccgtcgag ctcgaccgct ggatcggcct
     1201 cgccatggtg cgcggcgggc cggagcgcat cggcgagacc ttgcacgcca tatatccgct
     1261 gaagggcgag gcgccgccag tcaaactcgt ctcgccgcac cacgtcgatc cggagaacgc
     1321 ccgtgtccgt gcctagcccg acgaccgcgc tcgatgtgtc accgctcgcg ccgttctggc
     1381 ggcccggccg gcggggcgcc gacgtcgccg cgccgggcat cacgattgcc gagccgcgct
     1441 acgcgtacgc tcagctcatc gcccgccgcg gcacgggcac gcgtctggtc gaagggataa
     1501 ggtcaacgct cggcctcgcc ctccccgagc cctgccgcgc gacgcggagc cgcgatgtgc
     1561 gagcgctcga tatcggcccg ggcaattggc tgatcgcagc accacggcat gcggatgccg
     1621 aactgcaacg catgctgcgg tcagctgccg gtgacgccgg ctcggtgatc tcgcagtcgc
     1681 acggcaaggt gacactcgcg atcagtggac cagccacccg ggattgcctt gccaagggct
     1741 gccgtatcga tctgcatccg cgcgtgttcc tgccgggaca tgtcgccgtc acgccgatcg
     1801 gcccggtgaa cgcgatcgtc gccgcgctcg agccggcggg gagcttcgag ctcatcgtcc
     1861 cgtcgagcgt tgcagccgca ttcctcgagt ggctcgacac gtcggccgcc gagttcggtg
     1921 tggctctcgc ctgagctatt ctctgaaaag cccgtcggcc tcgaagcgcc ggaacgaggc
     1981 ctcgacgagc ttgccgagtt cgccgatcag cgcggcgtca ggcgccttca agttgaagca
     2041 ggacttgccc tgcatgcgct tcctgagttc cggcgaaatg ccggccagaa gctcgggatg
     2101 cgcataaacc gggatcagat gcaccgagac atagctcttc ctgatctcgg cgccgccgaa
     2161 ccaggtgcgg tagccgtcct tggtgcgcac ctgatgcgtg ccgatgtagt acttgcccgg
     2221 ctcgtcatgg agcaccacgc cggccttggc gtagcgcgcg aatgcaggtt tgatggccgc
     2281 aaagaccccg ccgaagctcg cggcagcccc tgtcccggtt cgcggcatgc tggatttcac
     2341 gtctcgcacg gatagtcggc agccgcgagc gccagcggct cgcaccagcg cttggcgtcc
     2401 acgcccttta cagcgacaca gcgtgcgcgg gcatcctcag ccgtcttgaa ggtgcccgcc
     2461 atcacgcgca tgtatctacg ccctccgacc agcaccggct cgcgcgagac gacgaccttg
     2521 agatcaccga gaccgaactg agcgagcact ccacgcacct cgtcgagcgc gcgcgcctcg
     2581 tcgaagcctt cgacgcgata ggccgcgatc ggaatcgcgt agcatgtcgc cgactggagc
     2641 ggtgctggat agtcacccgg aacgtagggc acgtcgtgat cgatgcgcga ggcctcgcgc
     2701 acctcggccg cgagccgccg tccaacctcc tcgaccttgc caacaagcgg cgctgcacta
     2761 ccgaaaaccg gcatgcggct ggaacgcgtc tgagcagaat gcagtgccag cgctagcacg
     2821 atgccgccga tggcaaaacc ggcgagagca taggtcaagc gaccgaatgt cagttccttc
     2881 ggcatcagca tcgccagctc ctcgccgggc agttgggttg gcagcgtgcc ccttgacagc
     2941 ccgacggcat ggcggacgca cgttcgggac tgctaggtcc gagacgccgc cgaaagtcga
     3001 gatcgccggc cccgcgccgg ccggacgccc acatgaacgt ccgttaatcc aaagtcgtag
     3061 atgcggattg tgattctttc aggccgtgat tgtggatgaa atgtgagtat ttcggacttt
     3121 cttgttgcca atctggtccg gatcggattc tcgcgcgcct cgccgcggca gtctggcgtt
     3181 gcatcgcaaa tcgtgtgaca cagcccctcg cccgatgccc gacgagcctg ccctgatcgg
     3241 ccgctcgccc gaaggttggg acacatcact tctgacggga ggcccgcatg gcgctcgcgc
     3301 tctacaacac gctcacccgg cgcaaggagc cgttcacgcc gatcgacccg gccaatgtgc
     3361 gcgtttacgg ttgcggcgtc accgtctacg accgcatcca catcggcaac ggccgcatga
     3421 tggtcgtgtt cgacctcctg ttccgcgtgc tccgccacgt ctacggcgag cgccacgtca
     3481 cctacgcacg caacatcacc gacgttgacg acaagatcaa cgcgcgcgcg gtggaagagg
     3541 gcgtcacgat ccgcgaactc acgcagcgca cgacgctctg gatgcacgag gacgtcgccg
     3601 gcctcggctg catgccgccg tcggtcgagc cgcgcgcgac cgaccacatc gaggagatga
     3661 agacactctg cgagatgctc gtccaacgcg ggcacgccta cgtcgccgaa agtcatgtac
     3721 ttttccacgt gccgagcatg gcggactatg gccgcctctc gaaccgtacg ctggaggaga
     3781 tgcaggccgg tgcccgcgtc gacgtcgcac cctacaaacg cgacgcgatg gacttcgtgc
     3841 tctggaagcc ctcgaagtcc ggcgagcccg cctggccgtc gccgtgcggc atcgcggagg
     3901 cgggccggcc aggctggcac atcgagtgct ctgcgatggc ggggcggcac ctcggccagg
     3961 tgttcgacat tcacggcggc ggcatcgacc tcgtctttcc gcaccatgag aacgagatcg
     4021 cacagtcgcg ctgcgcccac ggcacgcccg tgatggccaa cgtgtggatg cacaacggct
     4081 tcctcgaagc tgaggccggc gagaagatgt cgaagtcgct cggcaatttc atcacggtgc
     4141 gcgagctgct cgaccgggcg ccgggtgaag ccggccgcct cgccctcctc gcctcgctct
     4201 atcgccagcc gttgccctgg tccgagaagg gcgtcgtcga ggcgcgccgc acgctcgacc
     4261 attggtacga gctgacggct gacgcgactg acggcgcagc agtcgatcag gccgtcctcg
     4321 atgccttgct cgacgatctg aacacgccgg cggcgatcgc agcactccac gcgctccgct
     4381 cagacgccgc gaaaggctcg aagggcgccg cggccgcgct caaatcgtcg gcgcggctga
     4441 tgggcttcct tcagctctcg ccggctgact ggaagtcatg gcgccccgtt ggcatgacga
     4501 tcgacgaggc cgagatccgc cgcctgatcg cggcccgcct caccgcccgc aaggcgaagg
     4561 acttcaagga gggcgaccgc atccgcgacg cgctcgcggc ccaaggcatc gtgttgaagg
     4621 acggcaagga ccccacgact ggcgagatcg tcaccacttg ggaggcaaag gtgtgatgag
     4681 cgggagcgga cctccccatg cctgaacttc ccgaggtcga gacggtcagg cgcggcctcg
     4741 agccgacgct cgtcggtgct cgcatcgcgc gggtcgagca gcgccgtccg gacctgcgct
     4801 tcccgctgcc cgatcgcttc gccgcgcggc tcacggggtg ctccgtctcg tccctcgagc
     4861 ggcgcgccaa gtacatcatt gcgcacctcg acagcggtga agcgctgatc ctgcatctcg
     4921 gcatgacggg gcgtgtactg atttcgccgg tgggggatgc caaagcaatc atcggcgact
     4981 tcacgcacga tcacggcagc ctcgccgcgc acgatcacgt cgtcctgcac ctcgagagcg
     5041 gggccacgat cacctacaac gacgtgcgcc gcttcggctt catggacctc tgcgccgccg
     5101 acgcgctcgc cgacaacgcg cacctgaagg gtctcggaat cgagccgctc ggacccgagt
     5161 tgaccgccga ctggattgcg agcgccacca gcgggcggaa gagcccgctc aaggccttcc
     5221 tgctcgacca gcgcatcatc gccggcctcg gtaacatcta cgtctgcgag gcgctgtttc
     5281 gagcgggact gagcccgcag cgccgcgccg gcacgctcgc cgccc
//
LOCUS       DAORNH010000316         5319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11474_length_5319_cov_2.814780, whole genome shotgun sequence.
ACCESSION   DAORNH010000316 DAORNH010000000
VERSION     DAORNH010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11474_length_5319_cov_2.814780"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="livG"
                     /locus_tag="PK264_22085"
     CDS             <1..233
                     /gene="livG"
                     /locus_tag="PK264_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646805.1"
                     /note="Part of the ABC transporter complexes LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HRD78592.1"
                     /translation="GLNHDEVETLKAQIRRVRDVQQVTVLLVEHHMSLVMSVSDKVVA
                     IDFGRRIADGTPAEVQRNADVIKAYLGTGTVA"
     gene            230..967
                     /locus_tag="PK264_22090"
     CDS             230..967
                     /locus_tag="PK264_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78593.1"
                     /translation="MSAAPASRPVTLLEVEGLKAFYGQTQALHGVSFSLAEGGITALL
                     GANGAGKTTTLRAICGMVRAEGRVTLAGETITGRATERIVRLGVAHVPEGRGTFTTLS
                     VEENLRIAAYTRRDAAGVRRDLDRVLEYFPRLKERLSQQAGTLSGGEQQMLAISRALM
                     LSPRLMLLDEPSFGLAPLIVQEIFRIMRRINEDGKVSMLLVEQNAALALDLADHAYVL
                     ETGRVVMSGTAAEVKNDEGIRRSYLGY"
     assembly_gap    1152..1183
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1361..2569
                     /locus_tag="PK264_22095"
     CDS             1361..2569
                     /locus_tag="PK264_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78594.1"
                     /translation="MFIRTVAGNEGLAAFRAEVRAFCERELPAETRRKQLCGQHLEKA
                     EYDHWLKRLGTRGWLTGKWPTEHGGHGWAPEQFLAFQEELGRAGAPPVITFGPTMAGP
                     VIYTFGSAEHKRQHLPGIIRNDIWWCQGYSEPGAGSDLANLKTRALREGDHYVVNGQK
                     IWTSMAHWADWIFCLVRTDASGKKQDGISFLLIDMRTPGITVRPIIGINMGHHLNEVF
                     FDNVRVPVANLIGEEGKGWTYAKFLLANERVGNVDVAKLEHYLDQLRRLMAKTEEGGE
                     PITEDPLFRRRIAELEVGLATVKALMADQLAAARENGAPSLMGAAALKLRGTEVQQAI
                     LQTAVDVLGRHGLAFQRDALKAGWNGEQIGPEESPGLIYEHLLRRAATIYGGSSEVQK
                     NIIAKGALGL"
     gene            2587..3714
                     /locus_tag="PK264_22100"
     CDS             2587..3714
                     /locus_tag="PK264_22100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78595.1"
                     /translation="MPSFSAEERQLLHESLNDYLSSAYTFEHFRRLTKGEGEGFDRAA
                     WKSYAELGWLGVAIPEALGGAGGGVTELGIVMAAAGRHLVLEPLLATIALGATAIRIA
                     GRPDQQGLLQKVADGSLLLAFCHAEPRGGFARDYVRSTARRDGGRYVIDGEKTFALSA
                     HAADQLIVSARIGGGEGPIRLFLVPRDAGGVTCTNAPALDGRPGAALRLSGVSVDGAA
                     LLGAPDAVSAQIDIVHDWGIAAVCAEAAGAMAAVTEQTIAYLKTRQQFGQALSKFQVL
                     QHRLVDMSVAAEEARAATHAALQALDEGAADAGRIVAMAKVQTARSARFVGAQAIQLH
                     GGMGMTDELAIGHYYKRLSFGEAQFGDAEWHLKELAKALPL"
     gene            complement(3740..4066)
                     /locus_tag="PK264_22105"
     CDS             complement(3740..4066)
                     /locus_tag="PK264_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78596.1"
                     /translation="MSFDITRCGADWCGVKLGPDKSCGPVALRLAPVPQTAGELSGSL
                     SIDPAAQTYMVRATLLPASVGSAQTLRLIGNPNAPPQPFTRIIQFHDVLSRVSDAMCQ
                     AEAKVT"
     gene            4200..4856
                     /locus_tag="PK264_22110"
     CDS             4200..4856
                     /locus_tag="PK264_22110"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HRD78597.1"
                     /translation="MLGVAAAAMLKSIIAICPILVGLVAIATAAGLVKLPIELWLLDQ
                     RVPSIFRIHMAASGLAMIAIVVTLLLRAQPRWHRPLGRLAAVAILVGATTALPSALAS
                     EATIVARLGFASQAIAWLAALTIGVAAIRQRRIAFHRRAMLAMAAIASGAIWLRLATV
                     AAVVLDLPFDAVYAITAWAGWIVPLAAVLVVTEWLGASVVPSSPTTESGQGAARYGEA
                     "
     gene            4859..>5319
                     /locus_tag="PK264_22115"
     CDS             4859..>5319
                     /locus_tag="PK264_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78598.1"
                     /translation="MASSGARHWLLAALVAFAGAPAAAGQDALDMLEVSPAIVGRDNP
                     RDQLMRSVAGATEGRLRIQKAAETALALGDGPTLKAAVTAGSLAIVELPMASLEVEGA
                     IYLADQIPFLAVSFAEARTRYGALHRFLAERLSEEGLVLLYAVPRPPRGLLS"
BASE COUNT          837 a         1760 c         1830 g          860 t
ORIGIN      
        1 ccggcctcaa tcacgacgag gtggaaacac tgaaggcgca gatccgccgt gtgcgcgacg
       61 tgcagcaggt gactgtactg ctggtcgagc atcacatgag cctcgtcatg tcggtctccg
      121 acaaggtggt cgcgatcgat ttcggccggc gcatcgccga tggcacgcct gccgaggtgc
      181 agcgcaacgc agacgtgatc aaggcgtatc tcggcacggg aaccgtggca tgagcgcagc
      241 accggcgagc cggcccgtga cactcctcga ggtcgagggg ctgaaggcct tctacggcca
      301 gacgcaggcg ctgcacggcg tctccttctc gctcgccgag ggcggcatca cggcgctgct
      361 cggcgcaaac ggagcgggca agaccacgac cttgcgcgcg atctgcggca tggtccgcgc
      421 cgagggccgc gtcacgcttg ccggtgagac gatcaccggg cgggcgacgg agcgcatcgt
      481 ccgcctcggc gttgcgcacg tgcccgaggg ccgcggcacc tttacgacgc tctccgtcga
      541 ggagaacctg aggatcgccg cctacacgcg ccgcgacgcc gcgggcgtga ggcgcgacct
      601 cgatcgggtg ctcgagtatt tcccgcgcct gaaggagcgc ctctcccagc aggccggtac
      661 gctctcgggc ggcgagcagc agatgctggc gatcagccgc gcgctcatgc tctcgccgcg
      721 cctgatgctg ctcgacgagc cctcgttcgg cctcgcgccg cttatcgtgc aggagatctt
      781 tcgaattatg cggcgcatca acgaagatgg gaaagtgtcg atgctgctcg tcgagcagaa
      841 cgcggcgctc gccctcgatc tcgccgacca cgcctatgta ctcgagaccg ggcgcgtcgt
      901 catgtcgggc accgccgccg aggtgaagaa cgatgagggc atccgtcgct cctatctcgg
      961 ctactgaagg cagatatcag gttcatcgca gtacaattct cggggtgcga ggttaatcca
     1021 acccgcaatc gtttcccctg ccaagcatcg cgagagccgg cgtaccctct cgaggggtaa
     1081 gggccctggc cctccgcact ccggcctggg aaccagaatg gagaactgcc cccccgcagc
     1141 gcagccaact cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaaaagg cgggtggcct
     1201 gctgggtgcc cgccagcggg agcgcgtcac gccggaagcg tgcctccggc acggccctgg
     1261 ccctcgctcg ctcaacgaac ggatcggaaa atgttgtcag cgacgcgagc cgcgactacc
     1321 gtttgcaggt cagcgacagt cagcgacgac gggacgcccg atgttcatcc gaacggttgc
     1381 cggaaacgaa ggcctcgccg cctttcgcgc cgaggttcgc gccttctgcg agcgtgagtt
     1441 gcccgccgag acgcgccgga agcagctctg cggccagcac ctcgagaaag ccgagtacga
     1501 ccactggctg aagcggctcg gcacgcgcgg ctggctcacc ggcaaatggc cgaccgagca
     1561 cggcggacac ggctgggcgc ccgagcagtt tttggccttc caggaggagc tcggtcgtgc
     1621 cggcgcgccg ccggtgatca ccttcggtcc gaccatggcc gggccggtga tctacacctt
     1681 cggctcggcc gagcataagc gccagcacct ccccggcatc atccgcaacg acatctggtg
     1741 gtgccagggc tactcggagc cgggcgcggg ctcggacctc gccaacctga agacgcgcgc
     1801 tctccgcgag ggcgaccact acgtcgtcaa cggccagaag atctggacgt cgatggccca
     1861 ttgggcggac tggatcttct gcctcgtgcg caccgatgcc tccggcaaga agcaggacgg
     1921 catctcgttc ctgctgatcg acatgcgcac gcccggcatc acggtgcgcc cgatcatcgg
     1981 gatcaacatg ggccaccacc tcaacgaggt gttcttcgac aacgtgcgcg tgccggtcgc
     2041 gaacctgatc ggcgaggaag gcaagggctg gacgtacgcc aagttcctgc tcgccaacga
     2101 gcgcgtcggc aacgtcgacg tcgccaagct cgagcactac ctcgaccagc ttcgccgcct
     2161 catggcgaag accgaggagg gcggcgagcc gatcacggag gacccgctct tccgccgtcg
     2221 catcgccgag ctcgaggtgg gtctcgcgac cgtcaaggcg ctgatggccg accagctcgc
     2281 ggccgcgcgc gagaacggcg cgccctcgct gatgggggcc gctgccctga agcttcgcgg
     2341 taccgaggtg cagcaggcga tcctgcagac cgccgtcgac gtgctcggtc gccatggcct
     2401 cgctttccag cgcgatgcgc tgaaggctgg ctggaacggc gagcagatcg ggcctgagga
     2461 atctcctggt ctcatctacg agcatcttct ccgacgtgcg gccaccatct acggcggctc
     2521 gagcgaagtg cagaagaaca tcattgccaa gggcgcgctc gggctttgat cgccccggag
     2581 gacaccatgc cgagcttttc cgccgaggag cgccagctgc tccacgagag cctcaacgac
     2641 tatctgtcgt cggcctacac cttcgagcac ttcaggcgcc tgacgaaagg cgagggcgag
     2701 gggttcgatc gggcagcctg gaaatcgtac gccgagctcg gctggctcgg cgttgccatt
     2761 cccgaggcgc ttggcggggc gggcggcggg gtgacggagc tcggcatcgt catggcggca
     2821 gctgggcgtc acctcgtgct cgagccgctc ctggcgacca ttgcgctcgg tgcgacggcg
     2881 atccggatcg ccgggcgtcc cgatcagcaa gggctgctgc agaaggtcgc ggacggcagc
     2941 ttgctgctcg cgttctgcca cgctgagccg cgtggcggct tcgcgcgcga ctacgtgcgc
     3001 tcgacggcgc gtcgcgatgg tggacgatac gtgatcgacg gcgagaagac ctttgcgctg
     3061 tcggcgcacg cggccgatca gctgatcgtg tcggcgcgta tcggtggcgg ggagggaccg
     3121 atccggctgt ttctcgtccc gcgtgatgcc ggaggtgtca cgtgcaccaa cgcgccggcg
     3181 ctagacgggc ggccaggagc ggctttgcgc ctctccggcg tatccgtcga tggggctgcg
     3241 ctgcttggag cgcccgatgc cgtgtcggcg cagatcgaca tcgtgcacga ttggggcatc
     3301 gccgccgtct gcgcagaggc tgccggcgcc atggcggcgg taacggagca gacgatcgcc
     3361 tatctcaaga cgcgccagca gttcggccag gcgctctcga agttccaggt gctccagcat
     3421 cgcctcgtcg acatgagcgt tgcggcggaa gaggcgcggg ccgcgacgca tgccgcgctc
     3481 caggcgctcg atgagggcgc ggcagatgcg ggccggatcg tcgcgatggc gaaggtgcag
     3541 acggcgcgct cggcccgctt cgtcggcgct caggccatcc agctgcacgg cggcatgggc
     3601 atgacggacg aactcgccat cggccactac tacaagcggc tttccttcgg cgaggcgcaa
     3661 ttcggtgatg ccgaatggca cttgaaggaa ctcgccaagg cgctgccgct ctgagcgcga
     3721 gccgcccggt accgcgtcgt caggtgacct tggcctcggc ctggcacatc gcgtccgaca
     3781 cgcgggagag cacgtcgtgg aattggatga tccgcgtgaa gggctgcggc ggcgcattcg
     3841 gattgccgat caggcgaagg gtctgcgctg agcccacgct cgccggaagc agggttgcgc
     3901 ggaccatgta ggtctgcgcg gccgggtcga tcgaaaggct gccgctgagc tcacccgccg
     3961 tttgcggcac gggcgccagg cggagtgcca ccggcccgca gctcttgtcg ggcccaagct
     4021 tcacgccaca ccagtccgcg ccgcaccgcg tgatgtcgaa gctcatgccg agccgcttgc
     4081 tcgtccagcg gccggtgaga tcggcaggcg cgggctgcgg cagcgcagag gcgctcgaca
     4141 gcagcaagca aatcgccaca attggcgcct gaactgcggc ctggcgaagg aagcgtcgga
     4201 tgttgggtgt cgcggctgca gcaatgctca agtcgatcat agccatctgt ccaattcttg
     4261 tgggcctcgt cgcgatcgcg accgcggcgg gcctcgtgaa acttccgatc gagctgtggc
     4321 tgctcgacca gcgcgtgccg tcaatctttc gcattcatat ggctgcatca gggctcgcca
     4381 tgatcgccat cgtggtgaca ctgctgctgc gtgcgcagcc gcgctggcat cgcccgctcg
     4441 gcaggcttgc ggccgtggcg attctcgtcg gcgcaacgac tgcgctgccc tcggcattgg
     4501 cgagcgaggc gacgattgtg gcccgcctcg gctttgcgag ccaggcgatc gcctggctcg
     4561 cggcgctcac catcggagta gctgcgatcc ggcagcgccg gattgccttc caccggcgcg
     4621 ccatgctggc gatggccgca attgcctcgg gcgccatttg gctccgcctc gcaacggttg
     4681 ccgctgtggt tctcgacctg ccgttcgatg cggtctatgc gataactgcg tgggcgggct
     4741 ggatcgtgcc actcgcggcc gtgctggtcg tgacggaatg gctgggcgcg tcggtcgtgc
     4801 cgtccagccc gacgacagag tcggggcagg gtgcggcgcg ttacggagag gcttgacgat
     4861 ggcatcgagc ggcgcgcgac attggctcct ggcagctctc gtcgcatttg cgggagcgcc
     4921 ggccgccgcc ggacaagacg cgctcgatat gctcgaggtc agtccggcga tcgtcggccg
     4981 cgacaatcca cgtgaccagc tgatgcgcag tgtcgcgggt gcgacggaag ggcggctgcg
     5041 catccagaag gctgccgaga cggcgctggc gctcggcgac ggcccaaccc tcaaggcagc
     5101 ggtgacggcc gggtcgctcg ccatcgtcga gctgccgatg gcgagcctcg aggtggaggg
     5161 tgccatctac cttgccgatc agatcccctt ccttgccgtg tcgtttgccg aggcgaggac
     5221 acggtacggc gcgctgcacc ggttcctcgc cgagcggctc agcgaggaag ggctcgttct
     5281 cctctatgcc gtgccgcgac cgccgcgcgg cctgctctc
//
LOCUS       DAORNH010000317         5289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11556_length_5289_cov_2.531716, whole genome shotgun sequence.
ACCESSION   DAORNH010000317 DAORNH010000000
VERSION     DAORNH010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11556_length_5289_cov_2.531716"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2346)
                     /locus_tag="PK264_22120"
     CDS             complement(<1..2346)
                     /locus_tag="PK264_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase TIM-barrel-like
                     domain-containing protein"
                     /protein_id="HRD78599.1"
                     /translation="MATLALAAAGAAAGSALLPAGFSILGATISGAAIGSQIGAFAGS
                     FVDQALFGPSGQTRVLSGPRLSDLHVTASTEGAPIPRLYGRARLGGQIIWATDFEEEI
                     VRSSEGSGTGKGAPRPAAPSVTTVEYRYYANVAIALAEGEISGIGRVWADGREIDLAD
                     HTWRLYHGSETQLPDSLILAREGPDNAPAYRGTAYIVFEHLALAAFGNRIPQLSFEVL
                     RSVDPVNRLIRGVVLIPGSGEFVYAVDPVVRVAGGGASAPENTSTRQGGTNWRVALDQ
                     LEATLPNARSVSLVVSWFGSDLRAGQCELKPAVEIADKSTSPRSWSVAGLARATAPVV
                     SLSDGRPAYGGTPDDQSVVDAIRDLKARGLAVTLNPFILMDIAAGNSLVDPYTGSAGQ
                     PAYPWRGRITCDPAPGRPGSPDKTATGATQVASFIGTATPAHFAISGDTVTYAGPAEW
                     SLRRMVLHYAHLAKAAGGVDAFLLGSELRGLTTIRSATSAYPAVAALASLASDVKSVL
                     GPATKVSYAADWSEYFGHQPADGSGDVHFHLDPLWSSAGIDAVAIDLYWPLADWREGR
                     DHLDAADGTRSIYDLAYLRSNLRGGEGFDWYYASPADRNAQVRTPITDGAGKPWVFRY
                     KDLATWWQSQHFDRPGGSESGTPSSWVPQSKPVWLMEIGCPAVDKGPNQPNVFVDPKS
                     AETALPYFSLGTRDDLAQRRYIQALVEGLDPSDAGYVAGANPTSSVYGGPMIPLDHVH
                     AYAWDARPYPAFPDNAETWGDAANWRLGHWLNGRMSAVSLES"
     gene            complement(2346..2786)
                     /locus_tag="PK264_22125"
     CDS             complement(2346..2786)
                     /locus_tag="PK264_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRD78600.1"
                     /translation="MITSTRADVIAAARTWIGTPYHHQASLRGVGTDCLGLIRGVYEM
                     IIDRAAPPLPAYSRDWSEATGGESMLEAARIHLIERDRGLALPGDILVFRMRSGAIAK
                     HAAILATRSKIIHAMEGVAVAEVPLSPWWSRRIAGVFSYPGITA"
     gene            complement(2783..3670)
                     /locus_tag="PK264_22130"
     CDS             complement(2783..3670)
                     /locus_tag="PK264_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HRD78601.1"
                     /translation="MKTLPPGLQSHLDSGATTLCWCWRLTRRDGVRLGFTDHDLSLTF
                     DATTFEAAAGVTASEIRESVGLGVDNLEVSSVLSSTHLDERGLAAGLFDDATVEIFRV
                     NWATPDQRLLMRKGSLGEVRRSGQAFTAEVRGLAHYLQQPKGRLFQYSCDADLGDTRC
                     GVALTSPPLLVPGTITSAASPRLLTVSGLDAYEAGWFDRGLLRMTSGGAMGEAVEVKR
                     HTVGTTTVEIELWQPLAVAPAAADTFDIIAGCDKTFTTCRAKFSNGLRFRGFPHMPGN
                     DFVGSYPRADHRSASTEGP"
     gene            complement(3667..>4227)
                     /locus_tag="PK264_22135"
     CDS             complement(3667..>4227)
                     /locus_tag="PK264_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HRD78602.1"
                     /translation="VVLGSGHEERNARWSASRRSYNAGYGVKSIDDLYSVVEFFEERR
                     GRLYGFRWKDHNDWTSSRPGVPVTALDQEIGTGDGSRAAFQLTKRYGSAHAPFARDIR
                     KPVAGTVVLAVAGAPQTLGTDHSIDTTTGLVTFLAGHIPAPGALITAGFAFDVPVRFD
                     TDKLAVNLTGFRHGAIPSIPIVEIRL"
     assembly_gap    4228..4258
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4311..4892)
                     /locus_tag="PK264_22140"
     CDS             complement(4311..4892)
                     /locus_tag="PK264_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HRD78603.1"
                     /translation="MDEQGSGLTFDITADASSLRRELADVSRLGRQFGSVLTNAFEGI
                     ALRGRSLSDVFRTMGLSLSKLAFNAAMKPFESAIGSIFQNLLGGFSIGAPTATPAQAL
                     PFPFAKGGVIASPVSFPLGRGATGLAGERGAEAIMPLSRGPDGRLGVAASGQRSMTVT
                     FNVATPDVEGFRRSETQLSAMLARAVSQGQRNL"
     gene            complement(4913..5077)
                     /locus_tag="PK264_22145"
     CDS             complement(4913..5077)
                     /locus_tag="PK264_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HRD78604.1"
                     /translation="MAAGLGMLRLPPRDFWSATPRELAAAIRGLTGQAAPAPPPDRAA
                     LATLMHRYPD"
     gene            complement(5077..>5289)
                     /locus_tag="PK264_22150"
     CDS             complement(5077..>5289)
                     /locus_tag="PK264_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78605.1"
                     /translation="GLQVSDEAVSRMQCEGGATGYVDIVARLLAATFGTSEPPAEKPV
                     APPADAAWPASKEEERRPPDPFPGTR"
BASE COUNT          834 a         1552 c         2095 g          777 t
ORIGIN      
        1 gctctcgagc gatacggcgg acatgcggcc attgagccag tgcccaagcc gccaattcgc
       61 ggcatcgccc caggtctcgg cattgtcggg gaaggccggg taggggcgcg cgtcccaggc
      121 gtaggcatgg acgtgatcga gcgggatcat gggcccgccg tagacggagg aggtggggtt
      181 ggcgccggcg acatagcccg cgtcactcgg atcgaggcct tcgacgagcg cctggatata
      241 gcggcgttgg gcgagatcgt cgcgcgtgcc gagagagaag taggggagcg ccgtttccgc
      301 gctcttcggg tcgacgaaca cgtttggctg gttcgggccc ttgtcgacgg cggggcagcc
      361 gatctccatc agccagaccg gcttcgactg gggcacccag cttgatggtg tgccgctctc
      421 gctgccgccg gggcggtcga agtgctggct ctgccaccag gtggcgaggt ccttgtagcg
      481 gaagacccag ggcttgccgg cgccgtcggt aatcggagtg cggacttgcg cgttacggtc
      541 ggcagggctc gcgtagtacc agtcgaagcc ttcgccgccg cgcaggttgg agcgcagata
      601 ggcgaggtcg tagatcgagc gtgtgccgtc tgcggcatcg aggtggtcgc ggccctcgcg
      661 ccagtcggcg agcggccagt agagatcgat cgcgacggca tcgatgccgg cggacgacca
      721 gagcggatcg agatggaagt ggacatcgcc gctgccgtcg gcgggctggt ggccgaagta
      781 ctcggaccag tcggcggcat agctgacctt ggtggcgggg ccgagcacgg acttcacgtc
      841 ggaggcgagg ctcgcaaggg cggcgacggc gggataggcc gacgtggcgg agcggatggt
      901 cgtgaggccg cgcagttccg agccgagcag gaaggcatcg acgccaccgg cagcttttgc
      961 aagatgcgcg tagtggagca ccatccggcg gagcgaccat tcggcggggc cggcgtatgt
     1021 gacggtgtcg cctgaaattg cgaagtgggc gggtgtggcg gtgccgatga agctcgcgac
     1081 ctgagttgcc ccagtggctg tcttgtcggg gctgccgggg cggcccggcg cggggtcgca
     1141 ggttatgcga ccgcgccagg ggtaggcggg ctggccggcg ctgccggtgt agggatcaac
     1201 gagcgagttg ccggccgcga tatccatcag gatgaacggg ttgagcgtca ccgcgaggcc
     1261 gcgcgccttg aggtcgcgga tggcatcgac gacgctctgg tcgtccggcg tgccgccgta
     1321 cgcggggcgg ccatcgctga gactgacgac gggagctgtt gcgcgcgcga gaccggcgac
     1381 cgaccaggag cgcgggctcg tcgacttgtc ggcgatctcg acggccggct tcagctcgca
     1441 ttggccagcg cgaagatcgc tgccgaacca ggagacgacg agcgagacgg agcgcgcatt
     1501 cgggagcgtc gcctcgagct ggtcgagcgc caccctccag ttggtgccgc cctggcgggt
     1561 cgaggtgttc tcgggagcgc tcgcgccgcc accggcgacg cggactacgg gatcgacggc
     1621 gtagacgaac tcgccggagc cggggatcag cacgacgccg cggatgagcc ggttcaccgg
     1681 atcgacgctg cgcaagacct cgaaggaaag ctgcgggatg cggttgccga acgccgcgag
     1741 cgccaggtgc tcgaagacga tgtaggccgt gccgcgatag gccggcgcat tgtcagggcc
     1801 ctcacgggcg aggatcaggc tgtcggggag ctgcgtctcg ctgccgtggt agaggcgcca
     1861 ggtgtggtcg gcgaggtcga tctcgcggcc gtcggcccac acgcggccga tgccggagat
     1921 ctcgccttcg gcgagtgcga tcgccacatt ggcgtagtag cggtattcga cggtcgtgac
     1981 gctgggggct gctggacggg gggcgccctt gccggtgccg ctcccttcag aggagcgcac
     2041 gatctcctcc tcgaagtcgg ttgcccagat gatctgaccg ccgaggcggg cgcggccata
     2101 gaggcgcggg atcggcgcgc cctcggtcga ggcggtgacg tggaggtcgg agaggcgcgg
     2161 gccggagagg acgcgagttt ggcccgacgg accgaagagc gcctgatcga caaaggagcc
     2221 ggcgaaggct ccgatctgcg agccgatggc tgcgccggag atcgtggcac cgaggatcga
     2281 gaacccggcc ggcaggagcg cactaccggc ggcggctcca gcggcggcga gagcgagagt
     2341 tgccatcagg ctgtgatccc tgggtagcta aagacgccgg caatgcggcg gctccaccat
     2401 ggggagaggg gaacctcggc gaccgcgacg ccttccatgg cgtggatgat cttcgagcgg
     2461 gtggcgagga tggcggcgtg ctttgcgatg gcgcccgagc gcatgcggaa gacgaggatg
     2521 tcgccgggaa gcgcgaggcc gcgatcgcgc tcgatcaggt gaatgcgggc ggcctcgagc
     2581 atgctctccc cgcccgtcgc ctcgctccag tcgcgcgaat aggcggggag tggtggcgcc
     2641 gcgcgatcga tgatcatctc gtagacgccg cggatcaggc cgaggcaatc cgtgccgact
     2701 ccgcggagtg aggcctgatg gtggtagggc gtgccgatcc aggttcgagc cgcggcgatg
     2761 acgtcggcgc gcgtggacgt gatcatgggc cctccgtcga ggcgctgcga tgatcggcgc
     2821 gcgggtagga gccgacaaaa tcgttgccgg gcatgtgtgg gaagccgcgg aagcggagcc
     2881 cattcgaaaa cttggcgcgg caggtggtga aggtcttgtc gcagccggcg atgatgtcga
     2941 aggtgtccgc ggcggccgga gcgacggcga gcggctgcca gagctcgatc tcgaccgtag
     3001 tggtgccgac ggtgtggcgc ttcacctcga cggcctcgcc catcgctccg ccgcttgtca
     3061 tgcgcaggag gccgcgatcg aaccacccgg cctcgtaggc gtcgagcccg gagacggtca
     3121 gcaggcgcgg actcgccgcg ctcgtgatgg tgccaggtac gagcagcggg ggggacgtga
     3181 gtgcgacgcc gcagcgcgtg tcgccgaggt cggcatcgca gctgtactgg aagaggcggc
     3241 ctttcggctg ctgcagatag tgggcgaggc cgcggacttc cgcggtgaag gcctggcccg
     3301 agcggcggac ctcgccgagg gagcccttgc gcatcaggag gcgctggtcg ggggtcgccc
     3361 agttgacgcg gaagatctcg acagtcgcat cgtcgaagag gccggcagcg agaccgcgct
     3421 cgtcgagatg ggtggacgag aggacgctcg agacctcgag gttgtcgacg ccgaggccga
     3481 cgctctcgcg gatttcgcta gccgtgactc cggcagcggc ctcaaaggtc gttgcgtcga
     3541 aggtgaggct cagatcgtgg tcggtgaagc cgaggcggac gccatcgcgg cgcgtgaggc
     3601 gccagcacca gcaaagcgtg gtggcgccgc tgtccaggtg gctctggagg ccgggaggaa
     3661 gcgttttcac agccggatct cgacaattgg gattgagggg atggcgccgt gacggaagcc
     3721 ggtgagattg acagcgagct tatcggtgtc gaagcggacg gggacatcga aggcgaagcc
     3781 ggcggtgatc agggcgccgg gagccgggat gtgaccggcg aggaaagtga cgaggccggt
     3841 cgtcgtgtcg atggagtggt ccgtgccgag cgtttgcggc gcgcctgcaa cggcgaggac
     3901 gaccgtgccg gcaacgggct tgcggatgtc gcgggcgaag ggggcatgcg cgctgccgta
     3961 gcgcttcgtc agctggaagg cagcgcggct gccatcgcct gtgccgatct cctggtcgag
     4021 ggcagtgaca ggcacgccgg gacgcgatga tgtccagtcg ttatggtctt tccagcggaa
     4081 gccgtagaga cgaccgcggc gctcctcgaa gaactcgacg acggagtaga ggtcgtcgat
     4141 cgatttgacg ccgtagccgg cattgtagga gcggcgcgag gccgaccagc gggcgttgcg
     4201 ctcctcgtga ccggagccga gcacgacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     4261 cgagcgaaat gtctgtcggg aagcggatct catggaagga catcatcgcc tcacaggttg
     4321 cgctggccct ggctcacggc gcgggcgagc atggcggaga gctgcgtttc ggagcggcgg
     4381 aagccctcga cgtcgggtgt cgcgacgttg aaggtgaccg tcatgctgcg ctggccggac
     4441 gccgcgacgc cgaggcggcc gtcgggaccg cgggaaagcg gcatgatcgc ctccgcgccg
     4501 cgctcgccgg cgaggcccgt ggcgccgcga ccgagcggga agctcacggg gctggcgatg
     4561 acgccgccct tggcgaacgg gaatggaagg gcctgcgccg gggttgccgt cggggcgcca
     4621 atcgagaagc cgccgaggag gttctggaag atcgagccga tggcggattc gaacggcttc
     4681 attgccgcgt tgaaggcgag cttcgagagg gagagcccca ttgtgcggaa gacgtcgctg
     4741 aggctgcggc cgcgcagcgc gatgccctcg aaggcgttcg tcagcacgga gccgaactgg
     4801 cggccgaggc gcgagacgtc ggcaagctcg cggcggagtg aggaagcgtc ggccgtgatg
     4861 tcgaaggtaa gtccggagcc ttgctcgtcc atggaggtct cgtcgttggc tgtcagtcgg
     4921 gatagcggtg catcagcgtg gcgagcgcgg cgcggtcggg tggcggggcg ggggcagcct
     4981 gaccagtgag gccgcggatg gcggcggcca gctcgcgcgg ggtcgccgac cagaagtcac
     5041 gaggcgggag acggagcata ccgagccccg ctgccatcac ctcgtcccag ggaaagggtc
     5101 gggcgggcgg cgctcctcct ccttcgaagc gggccaggcg gcatcggccg gcggagcgac
     5161 cggcttctcg gcgggcggct ccgaggttcc gaatgtcgcg gcgaggaggc gggcgacgat
     5221 gtcgacgtag cctgtggcgc cgccctcgca ctgcatgcgc gagacggctt cgtccgagac
     5281 ctggaggcc
//
LOCUS       DAORNH010000318         5227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_11737_length_5227_cov_2.890371, whole genome shotgun sequence.
ACCESSION   DAORNH010000318 DAORNH010000000
VERSION     DAORNH010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_5227_cov_2.890371"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="PK264_22155"
     CDS             <1..1768
                     /locus_tag="PK264_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRD78606.1"
                     /translation="EKIEATTHAVPHGDRSGVPVEPWLTDQWYVNAAELAKGAMAAVR
                     SGKTTFVPERFANDYFRWMEAIEPWCVSRQLWWGHQIPAWYGPDGHVFVEMTEADAKA
                     AAARYYGKAADLKRDSDVLDTWFSSALWPFSTLGWPDKTPELARYYPTSTLVTGFDII
                     FFWVARMMMMGCEFMGKEPFRHVYIHGLVRDGQGAKMSKSKGNVMDPLDLIDGIGIDA
                     LIQKRIGGLKPGEAKKITQETRRDYPEGIAAYGADALRFSLAAMAAQGADVKFSVQRI
                     EGYRNFATKLWNAVRFAEVNGCQRVAGYDPKAATETLNRWIIGETERAAKAVTAGITE
                     MKFNEAAASIYAFVWDVYCDWFLELAKPIFASGSPAAQAETRATAAYVLDQILALLHP
                     FMPFITEELWEKTGETGPRRAGLLCLADWPTLAGLGNAAADAEIGWVMQLVKEVRSLR
                     TEMNVPAGAKIVVVITGASPETKARAGRHEDTVKRLARLDALSFADQPPKGSAQFVLA
                     EATVALPLAGIIDMAAEKVRLAKEIEKARVEIKKVDDKLANKAFVDKAPAEVVEENRE
                     RRAHFSEMATRLEAALKRIEAA"
     gene            1910..3088
                     /locus_tag="PK264_22160"
     CDS             1910..3088
                     /locus_tag="PK264_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78607.1"
                     /translation="MRVLIFSASSLWFFSGVAIAGPREECLEQFEPQSRIEACTEALK
                     ANPKDLEVLVARAMGNLFMGRTDEARADVNAGLAVDPKFAPLYDLRGAIHLYLEKTEL
                     AIADGTKALELAPDLHETRRWVAVALFDLNRRLEGLSLLTAGIRRYPNDPGIAKLYVA
                     RAQELQKFDGLVLALYDYGKVVELDGDHLTARIERAKIYRLLGDYQRAIADLAHVIEL
                     RGQKSGVLAIRDRLDLVTLLAATNDLAAAAKHAREVVEFLETEAAKKEVSEYDQLLWR
                     ARARITGNVEPEKALSDLDQIFKSSSDDADAYLYRAMLHNIAKRFDAALSDCKAAIYD
                     GPHDAEALRQIKLAHYQCGIAHEGRGAAANAVAAYKSAAAAPGLPEALVALKRLNASP
                     "
     gene            complement(3116..>4856)
                     /locus_tag="PK264_22165"
     CDS             complement(3116..>4856)
                     /locus_tag="PK264_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRD78608.1"
                     /translation="QRPEEQLFQSGGEAPKIKAFFARGEPRTGAGWAALASAHMAEGD
                     EAKAKAAAIKAWRHHDLPSTFETGFLERFGKYLTEADHRWRLDRILIDDVRWSSDRAD
                     KSAPARRLIALLPEAERPRAEARLAIYLKASLGPQMIAALPADPEGQTDWSLAYQRVQ
                     LLRRAGQHEAVAKILMSLPADSSLLVSPDDWFAERRLAAYEALRLGDAKRAYDLAKPI
                     AGLSVNPQKDATFLAGYIALVFLKDAKLAANDLAASLKAADGPLTQARAAYWHARALK
                     ALGDAAGAKAALELAARSVDTFYGLLALQELGKTASPFPRYPTEPTADEIVRFVSLDA
                     VRAVIVARKAGLDLSVTRAFLGHLRFVLKSEGEAALLGHLAEIVGDTQMAVRIGKAII
                     ARGQNLVYFSYPTHPMPAYTPLRPPPELAFLLGIARQESEFNPSTVSGAGARGLLQVM
                     PITAAHICRDYRIKCETQRLLTDKVYNATIASAYIGDRLGEFGGSYVLTLSGYNAGPG
                     RTRQWIREFGDPRDPAVDTIAWIERIPFEETREYVKKVLSNIQIYRFRLGEEGARIRI
                     VEDLARARRGAAP"
     assembly_gap    4857..4931
                     /estimated_length=75
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4932..>5227)
                     /locus_tag="PK264_22170"
     CDS             complement(<4932..>5227)
                     /locus_tag="PK264_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRD78609.1"
                     /translation="PAPAKADPPAAAAATTPPPAAAPPAPPRPPTPDELYMAKLDAAV
                     EPLRAIQLSDDVGQKLRTAVKSLQSGDPGPARALRGQLTDPTTRKLIEWLVLRAG"
BASE COUNT          893 a         1618 c         1773 g          868 t
ORIGIN      
        1 cgaaaaaatc gaggcgacga cgcacgccgt cccgcacggc gatcgctcgg gcgtgccggt
       61 cgagccgtgg ctcacggacc agtggtatgt gaacgcggcc gagctcgcca aaggcgcgat
      121 ggccgcggtc cgctcgggca agacgacgtt cgttcctgag cgcttcgcca acgactattt
      181 ccgctggatg gaggcgatcg agccctggtg cgtctcgcgc cagctctggt ggggccacca
      241 gattccggcg tggtatgggc cagatggcca tgtcttcgtc gagatgaccg aggcggatgc
      301 caaggcggca gcggcgcgct actacggaaa agccgccgac ctcaaacggg acagcgacgt
      361 cctcgatacc tggttctcct cggcactctg gccgttctcg acgctcggct ggccggacaa
      421 gacgccggag ctcgcgcgct actatccgac atccacactc gttaccggct tcgacatcat
      481 cttcttctgg gtcgcccgga tgatgatgat gggctgcgag ttcatgggga aggagccgtt
      541 ccgccacgtc tacatccacg gcctcgtccg cgacggccag ggcgccaaga tgtcgaagtc
      601 caagggcaac gtgatggacc ccctggacct catcgacggc atcgggatcg atgcgctcat
      661 ccagaagcgc atcggcggcc tgaagcccgg cgaagccaag aagatcacgc aggagacgcg
      721 gcgcgactat ccggagggca tcgctgccta cggcgcggat gcgctgcgct tctcgctcgc
      781 ggccatggcg gcgcagggcg ccgacgtgaa gttctcggtc cagcgcatcg agggctatcg
      841 caacttcgcg accaagctct ggaacgctgt gcgcttcgcc gaggtgaacg gctgccagcg
      901 cgtcgcgggc tacgacccga aggccgcgac cgagacgctc aaccgctgga tcatcggcga
      961 gacggagcgg gcggccaaag cggtcactgc cggcatcacg gagatgaaat tcaacgaggc
     1021 ggcggcgtcg atctacgcct tcgtgtggga cgtctactgc gactggttcc tggagctcgc
     1081 gaagccgatc ttcgcctcgg gctcgcccgc ggcgcaggcc gagacgcggg cgacggcggc
     1141 ctacgtgctc gaccagatcc tggcgctgct tcaccccttc atgccgttca tcacggagga
     1201 gctctgggag aagacgggcg agaccggccc taggcgcgcg ggcctcctct gcctcgcgga
     1261 ctggccgacg ctcgccggcc tcggcaacgc tgcggcggac gccgaaatcg gctgggtgat
     1321 gcagctcgtc aaggaggtgc gctcgctccg gaccgagatg aacgtgccgg cgggcgcgaa
     1381 gatcgtcgtc gtgatcacgg gcgcgtcccc cgagaccaag gcgcgcgccg gacgtcacga
     1441 ggatacggtg aagcggctcg cccggctcga cgccctttcg ttcgcggatc agccgccaaa
     1501 aggctcggca cagttcgtgc tcgccgaggc gacggtcgca ctgccgctcg ccggcatcat
     1561 cgacatggcg gcggagaaag tccgcctcgc caaggagatc gagaaggcgc gcgtcgagat
     1621 caagaaggtc gacgacaagc tcgccaacaa ggccttcgtc gacaaggcgc cggccgaggt
     1681 cgtcgaggag aaccgcgagc ggcgcgcgca tttcagcgag atggcgacgc gcctcgaagc
     1741 cgccctgaag cgcatcgagg cggcgtgacg gaggcgtgag cgagcctgcc aaagttgact
     1801 tccgcgaact ggcggaagca agatgggcgt gaagggcagc ttcaaactgt tggcgggctg
     1861 ccttggcttc agcgtgtgac gtccaattgg tccatcgcgg ggactgtcga tgcgcgttct
     1921 gattttctcc gccagctccc tctggttctt ctccggcgtg gccatcgccg ggccacgtga
     1981 ggaatgtctg gagcaattcg aacctcaatc gcgaattgag gcatgcaccg aggctctcaa
     2041 ggcaaatccc aaggatctcg aagtcctcgt ggcgcgcgca atgggcaacc tcttcatggg
     2101 ccgcacagat gaggcgcgcg ctgatgtgaa cgctggactt gcggtggatc caaaatttgc
     2161 gcccctctat gaccttcgcg gcgcaattca cctctacttg gaaaagacgg agcttgcgat
     2221 tgccgacggt acgaaagcct tggagctggc ccccgatctg cacgagaccc gacgctgggt
     2281 cgccgtcgct ctgttcgatt tgaacaggcg cctggaggga ctgtcactat tgactgctgg
     2341 aattcgtcgg tacccaaacg atcctggtat cgccaagcta tatgttgctc gagcccagga
     2401 gctgcaaaaa tttgacggtc tggtgctcgc gctctacgac tatggcaagg ttgtcgagct
     2461 ggacggtgac caccttaccg ctcgcatcga gcgagccaag atttatcgcc tgctcggcga
     2521 ttatcagcgc gcaatcgctg atttggcaca tgtgatcgaa ttgcgcggtc aaaaatccgg
     2581 agtgctcgca atccgggaca ggttggattt ggtgacgtta cttgcggcaa cgaacgattt
     2641 ggctgcggct gcgaagcacg ctcgtgaggt cgtcgagttt ttggagaccg aagccgcaaa
     2701 aaaagaggtc tcggaatacg atcaactctt gtggcgtgcg cgggcacgca tcacgggcaa
     2761 cgtagagccc gaaaaagctc tgagcgacct cgaccagata ttcaagagca gctcggacga
     2821 tgcagatgcg tatctctatc gtgccatgct acacaacatc gcaaagcgct tcgatgcagc
     2881 actatccgac tgcaaggctg cgatctacga cggcccgcac gacgcggaag cccttcggca
     2941 gataaagctc gcgcactatc agtgtggaat cgcccacgag ggtcgtggtg cggccgccaa
     3001 cgccgtcgca gcctacaaat cggctgcagc cgcgcccgga ctccctgagg cgctggtagc
     3061 gctcaagaga ctgaacgcat cgccttgacc ctcatcagct cggtgcggcg ctccatcagg
     3121 gcgccgcgcc gcgccttgcg cgcgcgagat cctcgacgat cctgatccga gcgccctcct
     3181 cgccgagacg gaagcggtag atctggatgt tcgagagcac cttcttcacg tactcgcgcg
     3241 tctcctcgaa ggggatgcgc tcgatccagg cgatcgtgtc gacggcggga tcgcgcgggt
     3301 cgccgaactc gcggatccat tgccgcgtgc ggccgggccc ggcgttgtat ccggagagcg
     3361 tgagcacgta ggagccgccg aactcgccga gacggtcgcc gatgtaggcg ctggcgatgg
     3421 tcgcgttgta gaccttgtcg gtgagcaggc gttgcgtctc gcacttgatg cgatagtcgc
     3481 ggcagatgtg cgcggccgtg atcggcatca cctggaggag gccgcgcgcg ccggcgccgg
     3541 acacggtcga cgggttgaac tcgctctcct ggcgcgcgat gccgagcagg aaggcgagct
     3601 cgggcggcgg acgcagcggc gtgtaggccg gcatcgggtg cgtcgggtag gagaaataga
     3661 cgaggttctg cccgcgcgcg atgatcgcct tgccgatgcg gacggccatc tgtgtgtcgc
     3721 cgacaatctc ggcgagatgc ccgaggagtg cggcttcgcc ttccgacttc aggacgaagc
     3781 gcagatggcc gagaaaagcg cgtgtcaccg agaggtctag ccccgccttg cgggcgacga
     3841 tcactgcgcg gacggcatcg agcgagacga agcggacgat ctcgtcggcg gtcggctccg
     3901 tcggatagcg cgggaagggt gaggccgtct tgccgagctc ctgcaatgcg agcaggccat
     3961 agaacgtgtc gacgctgcgt gccgccaact cgagtgctgc cttggcgccc gcagcatcgc
     4021 ccaatgcctt gagcgcgcgt gcgtgccaat aggcggcccg cgcctgtgtg agcggcccgt
     4081 cggcggcctt cagcgacgcg gcgagatcat tggcggcgag cttggcgtcc ttgaggaata
     4141 cgagcgcaat gtagcccgcg aggaacgtcg catccttctg tggattgacg gagaggccgg
     4201 cgatgggctt cgcgaggtcg taggcgcgct tggcatcgcc gagccttaat gcttcgtagg
     4261 cggcgaggcg ccgttccgcg aaccagtcgt cagggctcac cagcagcgag gaatccgccg
     4321 gcagcgacat caggatcttg gccaccgcct cgtgctgccc cgcacggcgg aggagctgca
     4381 cgcgctgata ggcgaggctc cagtcggtct ggccttcggg atcggcgggc agagccgcga
     4441 tcatctgcgg cccgaggctt gccttgagat agatcgcgag tcgggcctcc gcgcgcgggc
     4501 gctcggcttc aggcaagagc gcgatgaggc gccgcgcggg ggcggacttg tcggcccggt
     4561 cgctcgacca gcgcacgtcg tcgatcagga tgcgatcgag gcgccagcgg tgatcggcct
     4621 cggtgagata cttcccgaaa cgttcgagga atcccgtctc gaacgtgctc ggcaggtcgt
     4681 ggtggcgcca ggccttgatc gcggcggcct tggcctttgc ctcgtcgccc tcggccatgt
     4741 gggcagatgc cagggctgcc cagccggcgc ctgtgcgcgg ttcgcccctg gcgaagaagg
     4801 ccttgatctt cggcgcctcg ccgcccgact ggaaaagctg ctcctcgggc cgctgcnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn ncccgcgcgc agcacgagcc actcgatgag cttgcgcgtg gtcgggtcgg
     4981 tgagctgccc gcgcagtgcc cgcgccggac ccgggtcgcc cgactgcagc gacttcaccg
     5041 ccgtgcggag cttctggccg acatcgtccg atagctggat ggcgcgcagc ggctccactg
     5101 cggcatcgag cttggccatg tagagctcgt ccggcgtcgg cgggcgcggc ggagcgggtg
     5161 gggctgccgc gggcggtgga gtagttgcag ctgcggcggc aggaggatcg gccttggcgg
     5221 gcgctgg
//
LOCUS       DAORNH010000319         5138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12007_length_5138_cov_2.485147, whole genome shotgun sequence.
ACCESSION   DAORNH010000319 DAORNH010000000
VERSION     DAORNH010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12007_length_5138_cov_2.485147"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK264_22175"
     CDS             complement(<1..882)
                     /locus_tag="PK264_22175"
                     /inference="COORDINATES: protein motif:HMM:NF012486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HRD78610.1"
                     /translation="MSLLTRRSFFLAATATSAIIPFEIWLAEYGYAQYYGNNPPPKKK
                     TPPKGNTGPAPDPAPAPGNLRRPEATTPAGKANLVKYAEAVRRMRALPGSDPKSWTFQ
                     WYIHAVNSPQSEDDANEAQQRAKAAEIARLYGNSQTPERELAEATWGTCQTHFGGDGD
                     NFLPWHRMQTFFFERIIRSVLQDPSFVLPYWNYTSGSKALPVEFRQPNDQRLASLYIR
                     NRSANANGGRAIDAGQPSNRTLNNRFLSNRTYSNRGQVAGFCQQLDREHHGGVHVLVG
                     DERNMGDIPTAANDPIFW"
     gene            complement(921..1580)
                     /locus_tag="PK264_22180"
     CDS             complement(921..1580)
                     /locus_tag="PK264_22180"
                     /inference="COORDINATES: protein motif:HMM:NF023565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78611.1"
                     /translation="MLKPIIGLAVAAAVGAGGYYYYSKRQSPEASATAKIVAPGPATA
                     SKTDTKKSDSKGRVIKVNDPAARAPMARSKNEAPIELGDQPLRLVLDPPKGAAPQSLR
                     STATSLKAEQEVHLVIVNPRVTAATSVLYHVYLDLPGDGTQPSERHFVGSINFFGAVN
                     SKSETGISFPITDLVKELGDKKLLLDATSVTLVPGETPDKAALAKAKPTVEKIEIIVE
                     D"
     gene            complement(1861..2082)
                     /locus_tag="PK264_22185"
     CDS             complement(1861..2082)
                     /locus_tag="PK264_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HRD78612.1"
                     /translation="MKKLLIGAMFAALPMISAAGAAPTAVTPKLSTSSVTEAAYCRCG
                     VHRYCRRWDYGTCVSWGSRCRPCGGSYGY"
     gene            complement(2347..3045)
                     /locus_tag="PK264_22190"
     CDS             complement(2347..3045)
                     /locus_tag="PK264_22190"
                     /inference="COORDINATES: protein motif:HMM:NF006901.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRD78613.1"
                     /translation="MRGEDLEEMRGLRIFDGVSERQVEAMLAVSFLQRFPQGVELLRE
                     GDPADFLHVVIEGKVELFSTYRERETTVSVLGSGQCYIMAAVVLDRIYLKSARSLTSA
                     RVLLIPADAVRRCFAEDPAFARCLATELAEAYRMVVKELKNQKLRSGLERLANWLLAY
                     QSETGGARRFELPFEKKVLAARLGMVPEVLSRSFSALAAYKVRVDGPHVEIADSEALA
                     KLARPSVTIDDPST"
     gene            3296..>5138
                     /locus_tag="PK264_22195"
     CDS             3296..>5138
                     /locus_tag="PK264_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78614.1"
                     /translation="MAAGTPGTSEQQRALWLSTLAFTICFAVWTIFSIIGVQIKKDLN
                     LNDTQFGLLVGTPILTGSLIRLMLGVWTDQYGGRIVYTAVMLSAAVATWLLTFAYDYP
                     TFLLAALGVGIAGGSFAVGIAYVSKWFPKERQGTALGIFGAGNVGAAVTKFAAPFIMV
                     AYGWKTVANYWAIAIAVMGILFWLLTKDDPQLSERRRTGAKPEPLSAMLEPLRNVQVW
                     RFSLYYFFVFGAFVALALWLPRYLIGVYGLDIQTAGMIGAFYSVPASIFRIYGGHLSD
                     KYGARRVMYWTFGVSVLCCFFLSYPPTEYVVKGIRGPMAFRLETGLYAFIVLTFVLGF
                     FMSLGKAAVYKHIPVYYPKHVGSVGGVVGLVGGLGGFILPILFGLLNDLTGVWQSCFM
                     LLFGIVAVSLVWMHTSIRTMEREALGAELKKLPELPEMQEIHQPQHVGALSPKLIEDW
                     RPEDRDFWRTTGRAIARRNLLLSIPALLLSFAVWMVWSVVVAKLPQIGFTYSTDQLFW
                     LAALPGLSGATLRIFYSFMVPIFGGRLWTTLATWSLIIPALGIGWAVQNPDTPYWLFL
                     VLALLCGFGGGNFASSMSNISFFFPKAEKGNALALNAGLGNLGVSVVQ"
BASE COUNT          839 a         1606 c         1682 g         1011 t
ORIGIN      
        1 ccagaagatc ggatcgttgg cggccgtcgg gatatcgccc atgttgcgct cgtcgccgac
       61 gagcacgtgg acgccgccgt gatgctcgcg gtctagctgc tggcagaagc ctgcaacctg
      121 gccgcggttc gagtaggtgc gattggagag gaatcgattg ttgagtgtgc ggttgctcgg
      181 ctggcccgca tcgatggcgc ggcctccgtt ggcgttagcg gagcgattgc ggatgtagag
      241 cgaggcgagg cgctggtcgt tcggctggcg gaactcgacc ggcagtgcct tcgagccgga
      301 ggtgtagttc cagtagggca gcacgaacga ggggtcttgc agcacggagc ggatgatgcg
      361 ctcgaagaag aaagtctgca tccggtgcca gggcaggaag ttgtcgccat cgccgccgaa
      421 gtgcgtctgg caggttcccc aggtggcctc ggcgagctcg cgctcgggcg tctggctgtt
      481 gccgtagagg cgggcgatct cggcggcctt ggcgcgctgc tgcgcttcgt tggcgtcatc
      541 ctcgctctgc ggcgagttga cagcgtggat gtaccactgg aaggtccagc tcttgggatc
      601 gctgcccggc agcgcgcgca tgcggcggac ggcctctgca tatttgacga ggttcgcctt
      661 gcccgcgggc gtcgtcgcct cggggcgacg caggttgccg ggtgcgggtg ccggatcggg
      721 cgccggcccg gtgttgcctt tgggcggcgt cttcttcttc ggcggtggat tgttgccgta
      781 gtactgggca tagccgtact cggcgagcca gatctcgaac ggaatgatcg cggacgtggc
      841 cgttgcagcg aggaagaatg agcggcgcgt gagcagtgac atgagtgtct ccagggcagt
      901 gagctcagaa tgggggaggg tcaatcctcg acgatgatct cgatcttctc gaccgtcggc
      961 ttggccttgg cgagggcggc cttgtcaggc gtctcgccgg ggacgagcgt gacggaggtg
     1021 gcgtcgagca gaagtttctt gtcgccgagc tccttgacga gatcggtgat cgggaagctg
     1081 atgccggttt cggacttgga attgaccgcg ccgaagaaat tgatcgagcc gacgaagtgg
     1141 cgctcgctcg gctgggtgcc gtcgccggga agatcgagat agacgtgata gaggacgctc
     1201 gtcgccgcag tgacacgcgg attgacgatc acgagatgca cctcctgctc ggctttgagt
     1261 gaggttgccg tcgagcgcag cgattgcggc gcggcacctt tcggcggatc gagcacgagg
     1321 cgcagcggct ggtcgccgag ctcgatcggc gcctcgttct tggagcgggc catcggcgcg
     1381 cgtgcggcgg gatcgttcac tttgatgaca cgccctttgc tgtcggactt cttggtgtcg
     1441 gtcttggacg ccgtggccgg ccctggcgcc acgatcttcg cggtggcgct ggcctctggg
     1501 ctctggcgct tcgaatagta gtagtagccg cccgcgccga cagcggcagc gacggcgagg
     1561 cctatgatcg gtttgagcat gctgttcgcg cctatcgtgt gctcggctcg ctgcgcctcg
     1621 aggaggccga gagcgggaaa tcgaagtgtg ccggccagac ccgatttgca gagccgccgg
     1681 cgagcatttg aaacattcca gcacagatgt gcggcgtcaa taaatcaatg tcgatgtcgt
     1741 caatcgaaaa gtacagcaca gtagaaggtg ccagggctcc cgctgtaacc aggctagtca
     1801 agtcatgggc ggcattgccg gtgcgcaaca gccgtcgcgc accggcacca ggaggtagga
     1861 tcagtagccg tagctgccac cgcacggcct gcagcgcgag ccccagctga cgcatgtgcc
     1921 gtaatcccag cggcggcagt agcggtgaac gccacaccgg cagtaggccg cctcggtgac
     1981 cgacgacgtg ctgagcttcg gggtgaccgc ggtcggagca gcgccggcag cgctgatcat
     2041 gggcagtgcg gcaaacatgg cgccgatcag aagcttcttc atagcctaac tccaggttgg
     2101 ggcccccagt cggaatagaa aggggcttat cgcatcccgc cggtcgcggg cgcgaccggc
     2161 acagccgaaa gccgactgac gcgtgaagta tcagcgcgct tcgtccactc cgtcaaagga
     2221 attctccatg tctccaggtg attccgttac gaaaaatgct tgattcttca atgtcgatac
     2281 cttgtatgag cgttcattca cttgaccgat acatggttga tacgagcgag tccctcgcac
     2341 cagccgtcac gtcgacgggt cgtcgatcgt gacactcggg cgggcgagct tggcgagcgc
     2401 ctcgctgtct gcgatctcca cgtgcggacc gtcgaccctc accttgtagg cggcgagcgc
     2461 cgaaaacgag cgggagagga cctccggcac cataccgaga cgcgcggcca ggaccttctt
     2521 ctcgaacggc agctcgaagc gtcgcgctcc gcccgtctcg gattggtagg cgagcagcca
     2581 attggcgagg cgctcgagcc ccgagcgcaa cttctgattc ttgagctcct tcactaccat
     2641 gcggtaggcc tcggcgagtt cagtcgcgag acagcgtgcg aatgcgggat cctccgcgaa
     2701 gcagcgccgc acggcatccg ccggaatgag caggacacgc gccgaggtca gtgagcgtgc
     2761 cgacttcagg tagatccgat cgagcactac ggcggccatg atgtagcatt gtcccgatcc
     2821 gagcacggac acggtggtct cgcgctcccg gtaggtcgag aacagctcga ctttcccttc
     2881 gatcacgacg tgcaggaagt cggccggatc gccttcgcgc agcaactcga cgccctgggg
     2941 aaagcgctgc aggaacgaga cggcgagcat cgcttcgact tgccgctccg agacgccatc
     3001 gaagatgcgg aggccccgca tctcctcgag atcctcgcca cgcatgaagc tcctccgcct
     3061 cgcaatcttc atccaatcat caatgcaagg atcgacttga ttataatcaa gcaaatcacc
     3121 gatgcaattc gaaatggagg ctaaccaagt ggtcgtgatc tcctgacttg taccgcgagt
     3181 gctcttgaat ctccggcgcg gcacgacccg tttgacggcc gtcaactccg tcacggaagc
     3241 gtgatgacca ccctcgatcc gttcttttcg acgcgatccg aacgaggggg ccatcatggc
     3301 agccggcaca ccgggcacat ccgagcaaca acgggccttg tggctctcga cgctcgcttt
     3361 cacgatctgc ttcgcggtct ggacgatctt ctcgatcata ggcgtccaga tcaagaagga
     3421 cctcaatctc aacgataccc agttcgggct gctcgtcggc acgccgatcc tgaccggctc
     3481 gctcatccgc ctcatgctcg gcgtgtggac cgaccagtac ggcgggcgca tcgtctacac
     3541 ggcggtgatg ctctcggccg cggttgcgac ctggctcctc accttcgcct acgactaccc
     3601 gaccttcctt ctggcggcgc tcggcgtcgg catcgccggc ggctccttcg cggtcggcat
     3661 cgcctacgtc tcaaagtggt tcccgaagga gcggcagggc acggcgctcg gcatcttcgg
     3721 cgccggcaac gtcggcgcgg cggtcaccaa gtttgcggca cccttcatca tggtcgccta
     3781 cggctggaag acggttgcca actattgggc gatcgccatc gccgtcatgg gcattctctt
     3841 ctggctgctc accaaggatg acccgcagct ctccgagcgg cggcgcacgg gtgcgaagcc
     3901 cgagccgctg tcggccatgc tcgagcccct gcgcaatgtg caggtgtggc gcttctcgct
     3961 ctattatttc ttcgtgttcg gcgcgttcgt cgcgctggcg ctctggctgc cgcgctacct
     4021 catcggcgtc tacggcctcg acattcagac ggcaggtatg atcggcgcgt tctactcggt
     4081 gccggcgtcc atcttccgca tctatggcgg gcatctctcc gacaagtacg gcgcgcggcg
     4141 cgtgatgtac tggaccttcg gcgtctcggt gctgtgctgc ttcttcctgt cctatccgcc
     4201 gaccgagtac gtcgtgaagg gcatccgcgg gccgatggcg ttccgcctcg agacggggct
     4261 ctatgccttc atcgtactca ccttcgtgct cggcttcttc atgagcctcg gcaaggccgc
     4321 ggtctacaag cacatccccg tctactaccc gaagcatgtg ggctcggtcg gcggcgtcgt
     4381 cggcctggtc ggaggcctcg gcggcttcat cctgccgatc ctgttcggcc tgctcaacga
     4441 cctcaccggc gtctggcaga gctgcttcat gctgctgttc ggcatcgtcg ccgtgtcgct
     4501 ggtgtggatg cacacctcga tccgcacgat ggagcgcgaa gcgctcgggg ccgagctgaa
     4561 gaagctgccc gagcttcccg agatgcagga gatccatcag ccgcagcacg tcggtgcgct
     4621 cagtcccaaa ctaatcgagg actggcggcc ggaagatcgc gacttctggc ggacgacagg
     4681 cagggccatc gcgcggcgca acctgctgct ctcgatcccg gcgttgctcc tctcgttcgc
     4741 agtctggatg gtgtggtcgg tggtcgtcgc caagctgccg cagatcggct tcacgtactc
     4801 gaccgatcag ctcttctggc tcgcggcgct gccgggcctc tccggcgcga cgctgcgcat
     4861 cttctactcg ttcatggtgc cgatcttcgg cgggcgcctg tggacgacac ttgcgacctg
     4921 gtcgctgatc atcccggcgc tcggcatcgg ctgggccgtg cagaatccgg atacgcccta
     4981 ctggctgttc ctggtgctcg ccctgctctg cggcttcggc ggcggcaact tcgcctcctc
     5041 catgtcgaac atctcgttct tcttcccgaa ggccgagaag gggaacgcgc tcgcgctcaa
     5101 tgcgggcctc ggcaacctcg gcgtcagcgt cgtgcagt
//
LOCUS       DAORNH010000320         5079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12229_length_5079_cov_2.044188, whole genome shotgun sequence.
ACCESSION   DAORNH010000320 DAORNH010000000
VERSION     DAORNH010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12229_length_5079_cov_2.044188"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK264_22200"
     CDS             <1..526
                     /locus_tag="PK264_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78615.1"
                     /translation="VLLFSKNAYAAAFTSFYTFYLIERFAVSVPFAQVMLFLYLVVGA
                     IGVIIGGMIGDRIGRDRIIKISILGALPFALLLPFADLFWTGVLAVLISLIMSSAFAS
                     ILIYAMDLVPHRVGFVGGLFYGLAFGLGGIAAAALGFVADRAGVVGVFQLCAWLPALG
                     LLAYALPAPSRAGR"
     assembly_gap    549..584
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(603..>982)
                     /locus_tag="PK264_22205"
     CDS             complement(603..>982)
                     /locus_tag="PK264_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRD78616.1"
                     /translation="GHSLASAGLGQNGWGAGYLGEYGVMSAFVFELVATFLFVTVILG
                     STHNKAPGAVAGLAIGLTLIVIHIVGIQVTGVSVNPVRSLGPAVFVGSTAISQLWLFI
                     VAPMLGALLAGIVSKSGLLDPTS"
     assembly_gap    983..1008
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1009..1289)
                     /locus_tag="PK264_22210"
     CDS             complement(<1009..1289)
                     /locus_tag="PK264_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRD78617.1"
                     /translation="MNKATAEFIGTFTLVLLGCGSAVIAGKAVGVLGISIAFGLALIA
                     MAYGIGPVSGCHINPAVSLGALVAGRMSPGDFVTYVIAQCLGAIAGAGVL"
     gene            complement(2008..>2291)
                     /locus_tag="PK264_22215"
     CDS             complement(2008..>2291)
                     /locus_tag="PK264_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78618.1"
                     /translation="SSDDVAETFDALLREMQLITSRLTTEDWAVLKTIMTDPNGQLRV
                     DSIFDGFERETPAHELMRKLREAQFVRPANGGRWKNDSIIQIKPFGKLM"
     assembly_gap    2292..2322
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2424..>2637)
                     /locus_tag="PK264_22220"
     CDS             complement(2424..>2637)
                     /locus_tag="PK264_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78619.1"
                     /translation="EAVQPTYAQSTTRPASGAGTASRVRKPTLDEMTVGRTEVPVQRA
                     VTSARPNAYDNGKRRGRPRKTGRPGA"
     assembly_gap    2638..2660
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2661..>3567)
                     /locus_tag="PK264_22225"
     CDS             complement(<2661..>3567)
                     /locus_tag="PK264_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRD78620.1"
                     /translation="QVIRPTGLVDPVCEVRPAKTQVDDVIDEVRETAKKGYRTLCTVL
                     TKRMAEDLTEYMHEQGIRVRYMHSDIETLERIEIIRDLRLGAFDVLIGINLLREGLDI
                     PECALVAILDADKEGFLRSETSLVQTIGRAARNVDGKVVLYADSVTGSMERALAETNR
                     RREKQLAWNAANGITPESVKKSIADILQSVYENDRVTVDAGLAEDGAPLVGHNLAAVI
                     DDMEAKMRAAAADLEFETAARLRDEIKRLRETELAVAADPMATQGQVEDQAGAFEGAR
                     RYGGAPKERRGGTAPSTPARVSDPGP"
     assembly_gap    3568..3656
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3657..>4361)
                     /locus_tag="PK264_22230"
     CDS             complement(<3657..>4361)
                     /locus_tag="PK264_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRD78621.1"
                     /translation="CIYGIGSVETYTAMTFALKLGERISRDQLLADLVALHYRRNDGN
                     FHRGTFRVRGDTVELFPAHLEDRAWRITLFGDEVESIIEFDPLTGAKAGELELIKVYA
                     NSHYVTPKPTLQQAIKSIKSELKQRLDELHAAGKLLEAQRLEQRTLFDMEMMEATGSC
                     AGIENYSRYLTGRNPGDPPPTLFEYLPDNALVFADESHVTVPQIGGMFRGDYRRKATL
                     AEYGFRLPSCMDNRPLR"
     assembly_gap    4362..4558
                     /estimated_length=197
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4559..5072)
                     /locus_tag="PK264_22235"
     CDS             complement(<4559..5072)
                     /locus_tag="PK264_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRD78622.1"
                     /translation="MAPPVETAAQTPGLGEAPQSAFQVSGPPVLPALRSVAPRPEDFT
                     PNAALNALLSRPSERSERAQELLGRQPMLATHPLVSGDMPMFMPHRPPRPEKSEGGLH
                     FTLKSDFEPKGDQPQAIKELVTAAVAAERNQVLLGVTGSGKTFTMAHVIAATQRPALI
                     LAPNKALAAQL"
BASE COUNT          794 a         1478 c         1503 g          902 t
ORIGIN      
        1 cgtcctgctg ttctcgaaga acgcatacgc ggccgcgttc acctcgttct acacgttcta
       61 cctgatcgag cggttcgccg tctcggttcc gttcgcgcag gtgatgctgt tcctctatct
      121 ggtcgtgggc gcgatcgggg tgatcatcgg cggcatgatc ggcgaccgca tcgggcgcga
      181 ccggatcatc aagatctcga tcctcggcgc cctgcctttc gccctgctgc tgcccttcgc
      241 cgacctgttc tggaccggcg tcctcgccgt cctgatcagc ctcatcatgt cgagcgcgtt
      301 cgcatcgatc ctgatctacg ccatggatct cgtgccacac cgcgtgggct tcgtcggcgg
      361 gctcttctat ggactggcct tcgggctcgg gggcattgcc gcggcagcac tcggcttcgt
      421 tgccgaccgt gccggcgtcg tcggcgtgtt ccagctttgc gcctggctcc cggcgctcgg
      481 gctgcttgcc tacgcgttgc cagcgccgtc gcgcgccgga aggtgagcgg gcggggctcg
      541 agcttctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccccgc cacgtcccgc
      601 gatcaggatg tcggatcgag aaggccgctc ttcgatacga tgcccgcgag caaggctccg
      661 agcatcggcg cgacgatgaa gagccagagc tggctgattg cggtgctgcc gacaaacacg
      721 gccgggccca agctgcgcac cggattgacc gaaacgccgg tcacctggat gccgacgatg
      781 tggatgacga ttagcgtcag gccaatagct agaccagcca cggcacccgg cgccttgttg
      841 tgggtcgagc cgaggatcac ggtcacgaac aagaatgtgg cgaccagctc gaacacgaac
      901 gccgacatga cgccgtactc gccgagatag cccgcacccc aaccgttttg accaagtccc
      961 gcgctcgcga gcgaatggcc tgnnnnnnnn nnnnnnnnnn nnnnnnnnag cacgcccgcg
     1021 ccggcgatcg caccgaggca ctgagcgatc acgtaggtga cgaagtcgcc ggggctcatg
     1081 cgaccggcga cgagcgcgcc gaggcttacc gccggattaa tgtggcaccc cgagaccggg
     1141 ccgatgccgt acgccatggc gatcagcgcg aggccgaatg cgatggaaat cccgagcaca
     1201 ccgaccgcct tgccggcgat gaccgccgac ccgcagccga ggagcacgag cgtgaaagtg
     1261 ccgatgaatt ctgcagttgc cttgttcaat tccgttcccc cgatttggct cccagtggcg
     1321 ccgcgtgacg cgcacaacca tcgcgaccac cgagcgaccg gcctgggtgg cagccggcgt
     1381 ggcagccgcg cgctcccgac cgaatcgccg acagaaccgt acatgaattg cctagatgac
     1441 cagccagtct gcagggatag gtattatgcg atttcacacg gcaatgagct ggagtgagcg
     1501 cacgtctcgg caatgccagc attgccgaca catcgcacca tttctctgcg agataatatc
     1561 gagtactggt gtcaccagcg ggcatcgccg ccttgatcac tgttcaattc actgcctcgc
     1621 agcttcaccg ctttggcccg ataccggcac tccaattgaa ataataggga tgtttgcatg
     1681 ccttagcgat gtaagaccca actcccacaa tatgagataa cgaatctata catggaccgg
     1741 gtcgcccaag gacgtcatgc caagagttac atgcttgttg ggttgcgagg cgttggcaaa
     1801 accactctaa tgaaaaggag cctcagaatg gcagaggagc ataaatcctc agcgattgac
     1861 acggggcgca acaaatgaga tttgcgcatt cgtgcaggca tcacatctgc ttcacgatta
     1921 tgtcgccggc ggcacggact taattccgga agcagccctg gggactcctg ctgcgtcagc
     1981 tgcgagtttc tctgggccaa tctgcaacta catcaatttg ccaaatggct tgatctgaat
     2041 tatcgaatca tttttccagc gtccgccatt tgctggccgc acgaactggg cctctctcaa
     2101 tttcctcatg agctcatgag ctggcgtctc tctttcgaac ccatcgaata tgctatccac
     2161 tcgcaattga ccgtttggat cggtcatgat tgtcttaagt acggcccagt cttcggtggt
     2221 caacctggat gtaatcagtt gcatttccct gagcaaggca tcaaacgttt cggcaacatc
     2281 gtccgacgaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nncagaattg tggccactgg
     2341 ctcgttcctc aattcatact cggctatcga aaatgtaacc ggcacgtgac gctcgaagtg
     2401 cgaaatgatt gcctgcaagg ccctcaagcc ccaggccggc ctgtcttcct cggtcggccg
     2461 cgccgtttgc cattgtcata ggcgttgggg cgggccgagg tgaccgcgcg ctgcaccggt
     2521 acttcggtcc gaccgacagt catttcgtcg agggtcggct tgcgcacccg gctcgcggtg
     2581 cccgcgccgg aggctgggcg ggtcgtcgat tgggcatagg tcggctgcac ggcctcgnnn
     2641 nnnnnnnnnn nnnnnnnnnn gggtccaggg tccgagaccc tggcgggggt cgagggggcg
     2701 gtgccccctc gcctctcttt cggtgcacca ccatagcggc gggccccttc gaatgctccc
     2761 gcctgatcct cgacctggcc ctgcgtcgcc atcggatcgg ccgcgacggc gagctcggtc
     2821 tcgcgcagcc gcttgatctc gtcgcggagc ctcgccgccg tctcgaactc gagatcggcc
     2881 gcggcggcgc gcatcttcgc ctccatgtcg tcgatgacgg ccgcgaggtt gtgtccgacg
     2941 agcggcgcgc catcctcggc gaggcctgcg tcgacggtca cccggtcgtt ctcgtagaca
     3001 gactgcagga tgtcggcgat gctcttcttc acgctctcgg gcgtgatgcc gttcgccgca
     3061 ttccaggcga gctgcttctc gcggcgccga ttggtctcgg cgagcgcgcg ctccatcgag
     3121 ccggtgacgc tgtcagcgta gagcacgacc ttgccgtcga cgttgcgggc cgctcgaccg
     3181 atggtctgca cgagtgacgt ttcggagcgc aggaagcctt ccttgtcggc atccaggatg
     3241 gcgacgagtg cgcactcggg aatgtcgagg ccttcgcgca gcaggttgat gccgatcagt
     3301 acatcgaacg cgccgaggcg gaggtcgcgg atgatctcga tgcgctcgag cgtctcgatg
     3361 tcggaatgca tgtagcggac gcggatgccc tgctcgtgca tgtactcggt gagatcctcg
     3421 gccatgcgct tcgtcagcac tgtgcagagc gtgcgatagc ccttcttggc ggtctcacgc
     3481 acctcgtcga tgacgtcgtc gacctgggtc ttggcggggc gcacctcaca gacggggtcg
     3541 acgaggccgg tcgggcggat cacctgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgga
     3661 gcgggcgatt gtccatgcac gagggcaggc ggaagccgta ttcggcgagc gtcgccttgc
     3721 gccggtagtc gccgcggaac atgccgccga tctggggcac ggtgacgtgg ctctcgtcgg
     3781 cgaataccag cgcgttgtcg ggcaggtact cgaagagggt aggcggcggg tcgccgggat
     3841 tgcggccggt cagatagcgc gaatagttct cgatgccggc gcaggagccg gtcgcctcca
     3901 tcatctccat gtcgaacagc gtacgctgct cgagccgctg cgcctcgagt aacttgccgg
     3961 ctgcgtgcaa ctcgtcgagg cgctgcttca gctcggactt gatcgacttg atcgcctgtt
     4021 gcagcgtcgg cttcggcgtc acgtagtgcg agttggcgta aaccttgatg agctccagct
     4081 cgccggcctt ggcgccggtc agcggatcga actcgatgat gctctcgacc tcgtcgccga
     4141 acaacgtgat gcgccaggcc cggtcctcga ggtgggcagg gaacagctcg acagtatcgc
     4201 cgcgcacgcg gaaggtgccg cggtggaagt tgccgtcgtt gcgacggtag tgcagcgcga
     4261 cgaggtcggc gagcagctga tcgcgcgaga tgcgctcgcc gagcttcagc gcgaaggtca
     4321 tcgccgtgta ggtctcgacc gagccgatgc cgtagatgca cnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     4561 agctgcgccg cgagcgcctt gttcggcgcc aagatgagcg ccggtcgctg cgttgcggcg
     4621 ataacgtgcg ccatcgtgaa agtcttgccc gaaccggtga cgccgagcag cacctgattg
     4681 cgctcggccg caaccgccgc ggtgaccagc tccttgattg cctgcggctg atcgcctttt
     4741 ggctcgaagt ccgatttcag ggtgaagtga aggccgcctt cggatttctc cggccgcggc
     4801 ggacggtgcg gcatgaacat cggcatgtcg ccggagacga gcgggtgcgt cgcgagcatg
     4861 ggctggcggc cgagcagctc ctgcgcacgc tccgagcgtt ccgatggacg cgagagaagt
     4921 gcgttcaagg ccgcattcgg cgtgaagtct tctggccgcg gcgccacgga gcggagcgcc
     4981 ggcaggaccg gcggccccga cacctggaat gccgactgcg gagcctcgcc gaggcctgga
     5041 gtctgggcgg ccgtctcgac gggcggcgca atcggcttg
//
LOCUS       DAORNH010000321         5041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12341_length_5041_cov_2.695748, whole genome shotgun sequence.
ACCESSION   DAORNH010000321 DAORNH010000000
VERSION     DAORNH010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12341_length_5041_cov_2.695748"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PK264_22240"
     CDS             complement(<1..252)
                     /locus_tag="PK264_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD78623.1"
                     /translation="MSQHVELVGLAKQYGATVAVDGISLDVARGECVGLLGPSGCGKT
                     TTLRMVAGLIAPSGGSVKVLGRDLTHVPAHKRNMGYVFQS"
     gene            complement(264..1325)
                     /locus_tag="PK264_22245"
     CDS             complement(264..1325)
                     /locus_tag="PK264_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRD78624.1"
                     /translation="MAEIDRRGFLLAGAGTLLGTAVTPARAQQLTGTVTLMAYGGIFQ
                     DNYVETVVGPFTKAFPGVTVKYAPGGTSAQMLGTIRAQKADPQVDVVLMDVSTAGIAN
                     TEGLTDKMSATDVPSLAELYPEARYGDFGPAVTFDHLVLVYDTEKLTPPLAKLADLWR
                     PDLKGGLAISAPPNIQGLALTAMVEKMLGGDHTKSIDKAIAKLKELAPSVQTFEPNPD
                     GYALVLNGVVKAATGWNARAQLIRDQTKGKLGVLLPPEGSVFQINTINLVKGSKSRPA
                     AAAFINYALSQTAQKAFTERMFYAPTNGKATIAPAAIARTAAAADNRARMIPVDWAEL
                     AKVRDQWNNRWRREIIAAR"
     gene            1494..2900
                     /gene="leuC"
                     /locus_tag="PK264_22250"
     CDS             1494..2900
                     /gene="leuC"
                     /locus_tag="PK264_22250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRD78625.1"
                     /translation="MAPRTLYDKIWDSHVVHTYDDGTTILYVDQQLIHEVLSPQAFEG
                     LRLTNRRVRRPEATLAVADHNTPTTDGRSDETAEDDAKEQLAAIRKNCRDFGITFFGV
                     EDKRNGIVHVIGPEQGVTQPGMTLVCCDSHTATHGAFGALAFGVSTSEVEHVLATQTL
                     LATKSKNMRIRVDGRLPFGVTSKDLILAIIGKIGTAGGTGYVVEFAGEAVRALSMESR
                     MTMCNMAIEGGARAGLVAPDEKTFAYVKGRTFAPRGADWERAVAMWSQLPSDPDARYD
                     REVVLNGPDIAPMVTWGTNPEQVTSIDGTVPDPEAAPNADARKAMANALAYMGLTPGT
                     RIAEIKVDKIFIGSCTNSRIEDLREVAKVLKGRRIASHVAGIVVPGSQFIKQQAEEEG
                     IAEIVKAAGLEWRNAGCSMCLGMNPDRVPPGERCASTSNRNFVGRQGYKARTHLVSPV
                     MAAAAAIAGHFVDVRSWA"
     gene            2912..3535
                     /gene="leuD"
                     /locus_tag="PK264_22255"
     CDS             2912..3535
                     /gene="leuD"
                     /locus_tag="PK264_22255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRD78626.1"
                     /translation="MEKFTVLKGLAAPLAVINVDTDMIIPKQFLKGITRSGLGKHLFH
                     EMRFDEDGRENPAFVLNREPYRKAQIIVSGDNFGCGSSREHAPWAILDFGIRAIVAPS
                     FADIFYTNCFKNGILAVKLPQGDVAQLMADAESAASATMTIDLAAQTIRRHDGREIGF
                     EIDAFRKRCMLGGLDDIGLTLEHTEKIAAFEGRQRRSLPWLYDRKPA"
     gene            complement(3645..>5041)
                     /locus_tag="PK264_22260"
     CDS             complement(3645..>5041)
                     /locus_tag="PK264_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRD78627.1"
                     /translation="LSQDGERLISREVIVNDETGVIADQFYAERLYTRESITKLLEKT
                     GFRNIRHHGTAEAVSDRDQDLGMMARRILLTADAPQLPARKARGKVQAIDLTVLLGDP
                     RLPDQVKRNGTFNPEDLDTVRKLKDALSELPAYKVRYLNNHGTLDRDLSELKTDLVFN
                     LCDEGLNNDPFKELHVPAMLEVMGIPYTGAGPSALAACYDKGLVRAVALTLDVPVPLE
                     TYVRPGDQGATLPSVFPALLKPNQGDSSQGITKDAVVRNEKALLDYLEKLRTDFPKRG
                     VLVQEYLTGAEYSVGLIGNPEQGLRALPILEVDFSRLDPKLPKILGYESKWEPDSPYW
                     TQIRYHEAALADSSQSQLIEWSMRLFERLGCRDYARFDWRADARGEIKLLEVNPNPGW
                     CWDGKLNIMAGFQGVRYTELIAQILQSAEERLGIVAKSQPVVATAGATPNGSAGASQA
                     TQSGVSPQPGTSGS"
BASE COUNT          880 a         1697 c         1606 g          858 t
ORIGIN      
        1 cgactggaac acatacccca tgttccgctt gtgagctggt acatgtgtaa ggtcgcgccc
       61 gagaaccttg acgctgcccc cgctcggcgc gatcagcccc gcgaccatgc gcagcgtcgt
      121 agtcttgccg caccccgagg gaccgagcaa gccgacgcat tcaccccttg cgacgtccaa
      181 gctgatgccg tcgaccgcga cggtcgcgcc gtactgcttg gcgagcccga cgagctcgac
      241 atgttgggac atcgccgatc cgatcaacga gccgcgatga tctcgcgccg ccagcgattg
      301 ttccactggt cgcgcacttt cgccaattcg gcccaatcga ccgggatcat ccgcgcgcga
      361 ttgtccgcgg ccgccgccgt gcgcgcgatc gccgccggag cgatcgtcgc ctttccattg
      421 gtcggcgcat agaacatgcg ctcggtgaac gccttctgcg ccgtctgcga cagcgcataa
      481 ttgatgaagg ccgccgcggc cggccgcgac ttcgagccct tcacgaggtt gatcgtgttg
      541 atctggaaga cgctgccttc cggtggcagc agcacgccga gtttgccctt ggtctggtcg
      601 cggataagct gggcgcgcgc attccaccct gtcgccgcct tcacgacacc gttgagcacc
      661 agagcatagc cgtccggatt gggctcgaat gtctgtactg agggcgccag ttccttcagc
      721 tttgcgatcg ccttgtcgat cgatttcgta tggtcgccgc cgagcatctt ctcgaccatc
      781 gcggtgagcg cgagaccctg gatattcggc ggcgccgaga tggcgagccc gcccttgagg
      841 tccggccgcc agagatcggc tagcttggcg agcggcgggg tgagcttctc ggtgtcgtag
      901 acgaggacga ggtgatcgaa tgtgacggcc ggcccgaagt cgccgtaacg cgcctccgga
      961 tagagctctg cgagcgaggg cacgtcggtg gccgacatct tgtcagtcag cccttcggtg
     1021 ttggcgatgc cggcggtcga gacgtccatg agcacgacgt cgacctgcgg atcggccttc
     1081 tgggcgcgga tcgtgccgag catctgcgcc gaggtgccac ccggcgcata cttcaccgtg
     1141 acgcccggaa acgccttcgt aaacggcccg acgaccgtct cgacgtaatt gtcctgaaag
     1201 atgccgccat aggccatcag cgtgaccgtg ccggtcagct gctgcgcccg ggccggcgtc
     1261 actgccgtcc cgagcagagt gccggcgcct gcgagcagga accctctgcg atcgatctca
     1321 gccatatggt tcgccttcgc tccgccaatg tcagtcattc ctcgctcatc gaacatagcg
     1381 atcaccggac atgactgtct accatgagca gctcgcggcg ctcgcctctt gcgaggtgcg
     1441 gcgccggggt ggcagaagaa gtcagatccg gagcttccga caaccaggac accatggcgc
     1501 cccgcaccct ctacgataag atctgggaca gccacgtcgt gcacacctac gacgacggca
     1561 ccacaatcct ctatgtggac cagcagctca tccacgaggt gctctcgccg caagccttcg
     1621 aggggctgcg gctcacaaac cgccgcgtcc gccggccgga ggcgacgctc gccgtcgccg
     1681 accacaatac gccgaccact gacggtcgct cggacgagac cgccgaggac gacgccaagg
     1741 aacagctcgc cgcgatccgc aagaactgcc gcgatttcgg catcacgttt ttcggcgtcg
     1801 aggacaagcg caacggcatc gtgcatgtga tcggccccga acagggcgtt acgcagccag
     1861 gcatgacact cgtctgctgc gacagccata cggcgacgca cggcgcattc ggcgcgctcg
     1921 ccttcggcgt ctctacgtcg gaggtcgagc acgtgctcgc gacgcagacg ctgctcgcaa
     1981 ccaagtcgaa gaacatgcgc atcagagtcg atggccggct gccgttcggc gtcacgtcga
     2041 aggacctgat cctcgccatc atcggcaaga tcggcacggc cggcggcacg ggctacgtcg
     2101 tcgagttcgc gggcgaagcc gtgcgcgcgc tctccatgga gagccgcatg accatgtgca
     2161 acatggcgat cgagggcggt gcgcgtgcgg gcctcgtcgc gcccgacgag aagaccttcg
     2221 cctatgtgaa aggccgcacc ttcgcgccgc gcggcgcaga ttgggaacgg gccgtggcca
     2281 tgtggtccca gctgccgtcg gaccccgacg cgcgctacga ccgcgaggtc gtcctcaacg
     2341 gcccggacat tgccccgatg gtgacctggg ggacgaaccc cgagcaggtg acctcgatcg
     2401 acggcaccgt ccccgatccc gaggcggccc cgaacgcgga cgcgcgcaaa gccatggcga
     2461 acgcactcgc ctacatgggc ctcacgcccg gcacgcgcat tgccgagatc aaagtcgaca
     2521 agatcttcat cggctcatgc accaacagcc gcatcgagga cctgcgcgag gtggctaagg
     2581 tgctcaaggg acggcgcatc gcgagccacg tcgccggcat cgtcgtgcca ggctcgcagt
     2641 tcatcaagca gcaggccgag gaggagggca tcgccgagat cgtcaaggcg gccggcctcg
     2701 aatggcgcaa cgcgggctgc tccatgtgcc tcggcatgaa cccggaccgg gtgccgcccg
     2761 gcgagcgctg cgcgtcgacc tcgaaccgga acttcgtcgg caggcaaggc tacaaggcgc
     2821 gcacgcacct cgtcagcccg gtgatggcgg cggcggccgc gatcgccggc cacttcgtcg
     2881 atgtgcgcag ctgggcctga gggagacagc catggaaaag ttcacggttc tcaagggtct
     2941 cgcggcaccg ctcgccgtga tcaacgtcga caccgacatg atcatcccga agcagttcct
     3001 gaagggcatc acgcgaagcg gcctcggcaa gcacctcttc catgagatgc gcttcgacga
     3061 ggacgggcgg gagaaccctg cgttcgtgct caacagggag ccctaccgta aggctcagat
     3121 catcgtctcg ggcgacaact tcggctgcgg ctcctcgcgc gagcacgcgc cgtgggcgat
     3181 cctcgacttc ggcatccgcg cgatcgtcgc gccctcgttc gccgacatct tctatacgaa
     3241 ctgcttcaag aacggcattc ttgccgtgaa gctgccgcaa ggtgacgtgg cgcaactgat
     3301 ggccgacgcg gaaagcgccg caagcgccac catgaccatc gacctcgccg cgcagacgat
     3361 ccgtcgccac gacggccgcg agatcggctt cgagatcgat gccttccgca agcgctgcat
     3421 gctgggcggt ctcgacgaca tcggcttgac gctcgagcac accgagaaga tcgccgcctt
     3481 cgaggggcgc cagcggcgct ccctgccctg gctctacgat cgcaagcccg cctgagcgca
     3541 ggcggagcgg cctgagcaac gatctcgaca aatgacaatg ggcgccgcga gacctctctc
     3601 gcggcgccca tttcagcttc tcgacttggt ctaagcggac aggttcagct gccggacgtt
     3661 cccggctgtg gtgatacccc gctctgcgtc gcttgagacg cacctgcgga tccgttcggc
     3721 gtcgccccgg cagtggcgac gaccggttgc gacttcgcca cgattccgag tcgctcctcc
     3781 gccgactgca ggatctgggc aatcagctcc gtgtagcgga cgccttggaa gccggccatg
     3841 atgttcaact tgccgtccca gcaccagccc ggattcgggt tcacttcgag gagcttgatc
     3901 tctcctctcg catccgcgcg ccagtcgaag cgcgcgtagt cgcgacagcc gagccgctcg
     3961 aagaggcgca tcgaccactc gatgagctga gactgcgagc tgtccgccag cgccgcctcg
     4021 tgatagcgga tctgcgtcca gtacgggcta tcaggctccc acttcgattc gtaccccagg
     4081 atcttgggca gcttcggatc gagcctcgag aagtcgacct cgaggatggg cagcgcccgg
     4141 aggccctgct ccggattgcc gatgaggccg acggagtatt cagcgcccgt cagatactcc
     4201 tgcacgagca cgccgcgctt cgggaaatca gtgcgaagct tctcgaggta gtcgagcagt
     4261 gccttctcgt tcctgacgac ggcgtccttg gtaatgccct gcgagctgtc gccctggttg
     4321 ggcttcagca gcgcagggaa gaccgacggc agcgtcgcgc cttggtcgcc cggccgcaca
     4381 taggtctcga gaggcaccgg cacgtccagc gtcagagcga cggcgcgcac gaggcccttg
     4441 tcgtagcagg ccgccagcgc cgacggcccc gccccggtgt agggaatgcc catcacctcg
     4501 agcatcgccg gaacgtgcag ctctttgaag gggtcgttgt tcaaaccctc gtcgcagaga
     4561 ttgaagacga ggtcggtctt cagctccgag agatcacggt cgagcgttcc gtgattgttg
     4621 agatagcgca ccttgtaagc cggcagctcg gagagcgcat ccttgagctt gcggacggta
     4681 tcgaggtctt ccgggttgaa cgtgccgttg cgcttcacct ggtcgggaag ccgcgggtcg
     4741 cccagcagca cggtgaggtc gatcgcctgc accttgccgc gcgccttgcg ggcaggcagc
     4801 tgcggcgcat cggccgtgag tagaatgcgc cgcgccatca tgccgagatc ttgatcccgg
     4861 tcagagacgg cctcggccgt gccgtgatgg cgaatgttgc ggaagccggt cttctcgagc
     4921 aacttcgtga tcgattcgcg cgtgtacagg cgctcggcgt agaactggtc ggcgatcacg
     4981 ccggtctcgt cattgacgat cacctcgcgg ctgatcagac gctcgccgtc ctggctcaag
     5041 g
//
LOCUS       DAORNH010000322         5026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12392_length_5026_cov_3.147053, whole genome shotgun sequence.
ACCESSION   DAORNH010000322 DAORNH010000000
VERSION     DAORNH010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12392_length_5026_cov_3.147053"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PK264_22265"
     CDS             <1..650
                     /locus_tag="PK264_22265"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HRD78628.1"
                     /translation="LVQNPWRAPSYLLVVTISVPDRPDAHVTSAKAEVAFNPARVKSY
                     RRLGDEPRSVAATAARRAVSPAERRAVRLDGGTSVTSVYELTPVVADAEAAVGPDSAA
                     SALSAKRVTRSDPVETATVVLSFLAPGKAVPERRLVRLGLPTRLDEPASPALRGHGLA
                     IAAAAFAERLAGVDGGSAISIDDILRLTESSVSAAPSEAGQQFLAIVRRAKLAGW"
     gene            809..1813
                     /locus_tag="PK264_22270"
     CDS             809..1813
                     /locus_tag="PK264_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRD78629.1"
                     /translation="MTLLSKAAALSTALAVALGATFAVAQDMKFFRIGTGGAGGTYFP
                     IGGLIANAISAPPGSTPCDKGGSCGVPGLVAIAQSSNASVANVTAVESGQIESGLASA
                     DIVFQAYNGLAKFDKKFAKLRVIANLFPEHMHLVLPKGAKLGSIKELKGKRVGIFQAG
                     SGTQVVVLELLKLYGITKADIQAAELNPQQSADRLADGQLDAFFIVGGMPLAAISQLA
                     ATKGIELYSFSDEERATFLKAVPYYYAADIPAKSYEGVGYVAKSVAVGAQWVVSSSVD
                     EKLVYDITAALWNGNSRKLFDAGHSQAKNITLENALKAVGTPIHPGAAKYYKEKGLLK
                     "
     gene            1948..4053
                     /locus_tag="PK264_22275"
     CDS             1948..4053
                     /locus_tag="PK264_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HRD78630.1"
                     /translation="MTAPSTEPMIDARRAKEIEQEFESERLTRPIAPWTATFVFLFLI
                     VFAIYHYITAGTGIPIDHWHMGFHLTGVLLMVFILYPAQRFSAKGGAAPASGWLTPGG
                     VPLIDWAMGIAGGLAALWIGISWEGFDFSVLGETYKLAAQAMRQGNPADIDVVLGTIL
                     TVVVLEATRRTVGWVLPIIILVFIAFALFGPHMPFQILKHPGVTWKQFVNNIYFPAEG
                     IFGVALWIVSTVVFHFVLFGAVAQRMGLGQLFVDISTIVAGRFMGGPAKVSVVSSALF
                     GTISGSAVANVVSTGSLTIPNMKRLGYPGHFAGGVEAAASAGGQVTPPIMGAAAFIMA
                     EYLKMPYSQIAIAAAVPAFLHYFGVLTIVHFEAKRLGLKGHEAHEIPRLLPVLARGWP
                     ALLPLLVLLYVLFSGYTPYMAAFWGITTAIVVGLLNPMKRVHPREFLEAFHEGGRAAL
                     AVGAICAAVGIVIGVVTLTGLAFRLGFMVTGAAKDIGELIFGLSSLLPFEMLSLKDIT
                     LFLSMLFIGIACILMGAGVPTTALYIMLVTVAQPAFSNLGVPPLATHMFVLYYGIISE
                     VTPPVCTSAYAAAAIANSNPWKTGFTAFKLSNAKIMVPFVFCYSPVLLLVLPGFTWLA
                     FAQTFGTCLLGTICLGIAFVGYGWAPMPMPLRLLLALAGILLAMPSGGTDLIAIVLAA
                     LPIAQQVMAASRRTVTAGA"
     gene            4058..>5026
                     /locus_tag="PK264_22280"
     CDS             4058..>5026
                     /locus_tag="PK264_22280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRD78631.1"
                     /translation="MSGKPNILFLMADQLRWDYLACTGHPTIRTPNIDRMAARGTRFS
                     RAFVQSPVCGGSRMSFYSGRYNLTHGATYNNFPLRIDEKTMGDYLRPHGYRSVLVGKT
                     HFKPDTESLARLGIDPGTNPGLPYLQCGFEAFERDDGLHPEVDGKLAGADPAYNRWLR
                     QLGYSGANPWHSWANSVEGADGSVQSGWSMRNARRPARVKAEHSETAYMTDRAIAFLD
                     QVAGDPRPWMAHVSYIKPHWPYIAPAPYHAMYAERDVLPANRSAAELADPHPVIAAFM
                     RHEESVNFSREEVRQTVIPTYMGLIAELDHHVGRLLAHLEKRGLAEN"
BASE COUNT          773 a         1808 c         1544 g          901 t
ORIGIN      
        1 cccttgtgca gaacccttgg agagccccga gttacctgct cgtcgtcacg atctcggttc
       61 ctgaccggcc agacgcccat gtgacctccg ccaaggccga agtcgcgttc aacccggcac
      121 gcgtaaagag ctatcgccgc ctcggcgatg aaccccgttc cgttgcggcg accgcggcca
      181 ggcgggccgt gtcgccggcc gagcgccgag cggtccggct cgacggtggc accagcgtga
      241 catcggtcta cgagctgacg ccggtcgttg ccgatgccga agcggccgtc ggtcccgata
      301 gcgcggcctc ggcactctcg gcaaagcgcg ttacccggtc tgacccggtc gagaccgcaa
      361 ccgtcgtcct ctccttcctg gctcccggaa aagctgtgcc ggagcgtcgc ctggtccggc
      421 tcggattgcc gacgcgcctc gacgagccgg caagccccgc gctccgcgga cacgggctgg
      481 ccattgccgc cgcggctttt gccgagcggc ttgcgggtgt cgacggcggc agcgcgatca
      541 gcatcgatga tattcttcgg ctgaccgaga gctcggtctc cgcggccccg agcgaggccg
      601 ggcaacagtt tcttgcaatc gtccgccgcg caaagctggc cggctggtag gtcgcccgcc
      661 tccatgctga cacagccgct tcacaggcgc gctcaaggct ctatgatgat gggccagcgc
      721 gtcggcccca atcggccgac cgcctggggt ccgccggatt gtcggcgccg accccggaca
      781 gggactacgg gacccaggga gagtgacctt gacgcttctc agtaaagcag ccgcgttgtc
      841 gacggccctc gccgtcgcgc tcggcgcaac gttcgccgtc gcgcaggaca tgaagttctt
      901 ccgcatcggc acaggcggtg ccggcggcac atacttcccg atcggcggcc tcatcgccaa
      961 tgcgatctct gctccgcccg gctccacgcc ctgcgacaag ggcggctcgt gcggcgtccc
     1021 gggcctcgtc gcgatcgccc aatcgtccaa cgcgtcggtc gccaacgtga ccgcagtcga
     1081 gtccggccag atcgaatccg gtctcgcctc cgccgacatc gtcttccagg cctacaacgg
     1141 cctcgccaag tttgataaga aattcgcgaa gctgcgcgtg atcgccaacc tcttccccga
     1201 gcacatgcac ctcgtgctgc cgaagggcgc caagctcggc tccatcaagg agctgaaggg
     1261 caagcgcgtc ggcatcttcc aggccggctc cggcacgcag gtcgtcgtgc tcgagctcct
     1321 gaagctctac ggcatcacca aggcggacat ccaggccgcc gagctcaacc cgcagcagag
     1381 cgccgaccgc ctcgccgacg gtcagctcga cgccttcttc atcgtcggcg gcatgccgct
     1441 ggccgccatc tcgcagctcg ccgcgacgaa ggggatcgag ctttactcgt tctcggatga
     1501 ggagcgggcg accttcctga aggccgtgcc ctactactac gccgccgaca tcccggcgaa
     1561 gagctacgaa ggcgtcggct acgtcgcgaa gtcggtcgca gtcggcgcgc agtgggtggt
     1621 gagcagctcc gtcgacgaga agctggtcta cgacatcacc gcggcactct ggaacggcaa
     1681 ctcgcgcaag ctcttcgatg cgggccactc gcaggcgaag aacatcacgc tcgagaacgc
     1741 gctgaaggcc gtcggcacgc cgatccatcc gggggcggcg aagtactaca aggagaaggg
     1801 gctcctgaag taagcttcgc cggccttgcc gactgacgca cgcggggtcg ggtccttgcg
     1861 gtccggcccc gcgcatcttt cgccgcttgc caaccggctt ggcagcaggc ccccgtgaac
     1921 tcgcctgcca taccatcagg accgcccatg accgctccct ccactgagcc gatgatcgac
     1981 gcgcgccgcg ccaaggaaat cgagcaggag ttcgagagtg agcggctgac gcgaccgatt
     2041 gcgccgtgga cggcgacgtt cgtcttcctc ttcctgatcg tgttcgcgat ctaccactac
     2101 atcaccgccg gcaccggaat tcccatcgac cattggcaca tggggttcca cctcaccggc
     2161 gtgctgctga tggtattcat cctctatccg gcacagcgct tcagcgcgaa aggcggcgcc
     2221 gccccggcat cgggctggct cacgcccggc ggcgtccccc tgatcgactg ggcaatgggc
     2281 atcgccggcg gcctcgcggc gctctggatc ggcatctcct gggaaggctt cgacttttcg
     2341 gtcctcggcg agacctacaa gctcgcggcg caagcgatgc gccagggcaa ccccgccgac
     2401 atcgacgtcg tgctcggaac gatcctcacc gtcgtcgtgc tcgaggcgac gcggcgcacc
     2461 gtcggctggg tgctgccgat catcatcctc gtcttcatcg cctttgccct cttcgggccg
     2521 cacatgccct tccagatcct gaagcaccct ggcgtcacct ggaagcagtt cgtcaacaac
     2581 atctacttcc ccgccgaggg gatcttcggc gtggcgctct ggatcgtctc gacggtcgtc
     2641 ttccacttcg tgctgttcgg cgcggttgca cagcgcatgg gtctcggcca gctgttcgtc
     2701 gatatctcga ccatcgtcgc cgggcgcttc atgggtggtc ccgcgaaggt ctccgtcgtc
     2761 tcctcggcgc tcttcggcac catctcgggt tcggccgtcg caaacgtcgt ctcgaccgga
     2821 tcgctgacca tccccaacat gaagcgcctc ggctatccgg ggcacttcgc gggcggcgtc
     2881 gaggccgcgg cgagtgcggg cggacaggtg acgccgccga tcatgggcgc cgccgccttc
     2941 atcatggccg aatacctgaa gatgccctac agccagatcg cgatcgcggc ggccgtgccg
     3001 gcgttcctcc attatttcgg cgtgctgacg atcgtacatt tcgaggcgaa gcggcttggc
     3061 ctcaagggcc acgaggcgca cgagatcccg cgcctcctcc ccgtgctcgc ccgcggctgg
     3121 ccggcgctct tgccgctgct ggtcctcctc tatgtgctct tctcgggcta cacgccctac
     3181 atggcggcct tctggggcat caccacagcg atcgtcgtcg gcctcctgaa cccgatgaag
     3241 cgggtgcatc cacgcgagtt cctggaagct ttccacgagg gcgggcgcgc ggccctcgcg
     3301 gtcggcgcca tctgcgcggc ggtcggcatt gtcatcggcg tcgtgacgct caccgggctc
     3361 gcctttcggc tcggcttcat ggtgacgggg gcagccaagg acatcggcga gctgatcttc
     3421 gggctctcga gtctgctgcc cttcgagatg ctgtcgctga aggacatcac gctcttcctg
     3481 tcgatgctct ttatcggcat cgcctgcatc ctgatgggcg ccggcgtgcc gacgaccgcg
     3541 ctctacatca tgctggtgac agtggcgcag cccgctttct cgaacctcgg cgtgccaccg
     3601 ctcgcgaccc acatgttcgt cctctactac ggcatcatct cggaggtgac gccgcccgtc
     3661 tgcacctcgg cctacgcggc ggcagcgatc gccaactcga acccgtggaa gacgggcttc
     3721 accgccttca agctctcgaa cgcgaaaatc atggtgccgt tcgtgttctg ctacagcccc
     3781 gtgctgctgc tcgtccttcc gggcttcaca tggctcgcct tcgctcagac gttcgggacg
     3841 tgtctcctcg gcacgatatg cttgggtatt gctttcgtcg gatatggctg ggcgccgatg
     3901 ccgatgccgc tgcggctgct cctggcgctc gccggcatcc tgctcgccat gccgagcggg
     3961 gggaccgatc tcatcgccat cgtacttgcc gcgctgccga ttgcgcagca ggtcatggcg
     4021 gcgtcccggc gcaccgtgac tgctggggca tagcgagatg agcgggaagc ccaacatcct
     4081 tttcctgatg gcagaccagc tccgttggga ctatctcgcc tgcaccgggc acccgacgat
     4141 ccgcacgccc aacatcgatc gcatggctgc tcgcggtacg cgcttttccc gcgcctttgt
     4201 gcagtcgccg gtctgcggcg gctcgcgcat gagcttctat tcgggccgct acaatctcac
     4261 acacggtgcc acgtacaaca acttcccgct gcgcatcgac gagaagacga tgggcgacta
     4321 tctccgcccg cacggttacc gctccgtgct cgtcggcaag acgcacttca agccggacac
     4381 ggagagcctc gcccgcctcg gcatcgatcc cggtaccaac ccggggctcc cctatctcca
     4441 atgcggtttc gaggccttcg agcgcgacga cggactccat ccggaggtcg acggtaagct
     4501 cgcgggcgct gatccggcct acaatcgctg gctccgccag cttggctact ccggcgcgaa
     4561 cccctggcac agctgggcga actcggtcga gggcgcagac ggctccgttc agtccggctg
     4621 gtcgatgcgc aatgcccgcc gccccgcgcg cgtcaaggcc gagcattccg agactgccta
     4681 catgacggac cgcgccatcg ccttcctcga ccaggtcgcg ggcgatcctc ggccatggat
     4741 ggcgcacgtc tcctacatca agccgcactg gccctacatc gcacccgcgc cctatcacgc
     4801 gatgtatgcc gagcgcgacg tccttcccgc caaccgcagc gccgccgagc ttgccgatcc
     4861 gcacccggtg atcgccgcct tcatgcggca cgaggagagc gtcaacttct cccgcgagga
     4921 ggtgcgccag acggtgatcc cgacctacat ggggctgatc gccgagctcg accatcacgt
     4981 cgggcgtctc ctcgcccatc tcgagaagcg cgggctcgcc gagaac
//
LOCUS       DAORNH010000323         5023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12404_length_5023_cov_2.460548, whole genome shotgun sequence.
ACCESSION   DAORNH010000323 DAORNH010000000
VERSION     DAORNH010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12404_length_5023_cov_2.460548"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="PK264_22285"
     CDS             complement(<1..2252)
                     /locus_tag="PK264_22285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRD78632.1"
                     /translation="MTIATEGLTTRATGWRWPVVLTLALRELRSGLSGFYVFLACIAL
                     GVAVIAGVGALSDALRAGFERQGEALLGGDVTLARSHKAATEAERRWIATHGKVSEAA
                     TLRSMARTAAPAAGADEQALIELKAIDDLYPLSGTLTLSSGVTASTALAGRGAAVEPI
                     LLDRLGIKLGDRIKLGRTEVEVRATIVAEPDKIADRLTYGPRVLVSLDTLKASQLAEP
                     GSLVRWRYALKLAAAGPTADNADLVRFRDKAKADLPESGFIIADRRDPSPSVSRTLER
                     LRQFLTLVGLTSLIVGGVGVASAVATYMDRRRKVIATLKALGARQRTITALHLVQILI
                     IGLLGIGIGLGLGMLMPLALDATIGAALPIRGEFVISARSVLTAIAYGLLVTLLFTVW
                     PLGRAGRIRAASLFRDDVQPARTSLPWTSIASLIVIAALLAVLAILSSEDRRIASFFL
                     LGIAGVFAVFLGLGQLVTLVARTLPRPRRPELSLALGSLGAPGGLTRSVVLALGSGLS
                     LLVTLSLADASIRDELTARIPKGSPNYFILDLPRDDRDRHDAIVKRHVAAAEVRHAPM
                     LRGRLVKLGDRPAETVKASPKASWVLQGDRGLTYAEDVPEGSKVTAGAWWPKDYQGEP
                     LVSFEGELAKDLGLKIGDSVTVNILGRDVTARIASFRELKWESLAINFVMVFSPNTLS
                     GAPHNLLATITLPADTALVTEAAIARDLGRELPGTTAIRVKDAINAFGTLFAKIMTAV
                     RVAAGITLAA"
     gene            complement(2249..2995)
                     /locus_tag="PK264_22290"
     CDS             complement(2249..2995)
                     /locus_tag="PK264_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78633.1"
                     /translation="MQDATPASEHGAIGAGARAVVSLANVSLSLTSRAGSVDILRNVS
                     LDIGEGETVAVVGPSGSGKTSLLMIIAGLERATRGSVSVVGQDFSALGEDQLALVRGA
                     AIGIVFQSFHLVPTMTALENVALPLELAGRRDIVPLVEARVLLGEVGLGHRVDHFPAE
                     LSGGEQQRVAIARALVTRPKLLLADEPTGNLDGQTGETVVGLLVDLQKRRRTTLVLIT
                     HDHALARRCGRTVHMADGRIVSRVATGALA"
     gene            complement(3130..3570)
                     /locus_tag="PK264_22295"
     CDS             complement(3130..3570)
                     /locus_tag="PK264_22295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78634.1"
                     /translation="MCLVLYFGSERPIEVQAIATAFGDVGLVADVRPDVHAVIGTAHS
                     VRVTTEWDGCCCSALDDFKAYFDPGEPDDVDQDRKHTAMLAALARLIESLAQLRGTRL
                     VALWDGGTLLPAKLMPPLDVTPLDVSVISNETMAFSLIYPLRTA"
     gene            complement(3577..4551)
                     /gene="pdhA"
                     /locus_tag="PK264_22300"
     CDS             complement(3577..4551)
                     /gene="pdhA"
                     /locus_tag="PK264_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HRD78635.1"
                     /translation="MVPAFTREQELAAYREMLLIRRFEERAGQLYGMGLIGGFCHLYI
                     GQEAVVVGMQMAIRPSDQVITTYRDHGHMLACGMDPKGVMAELTGRRSGYSRGKGGSM
                     HMFSKEKNFYGGHGIVGANVPLGAGLAFANKYRGNDSVCLTYFGDGAANQGQVYESFN
                     IAKLWSLPVIFIIENNRYAMGTAIERAAATTNFSLRGSSFDIPGEQVDGMDVRAVKAA
                     GDKVVAHARAGNGPYILEMMTYRYRGHSMSDPAKYRTREEVERVREERDPIERVRKRL
                     LDGRLASEDELKAIDGQIRQIVTVAAEFSQSDPEPDPSELYTDVLIAS"
     gene            complement(4784..>5023)
                     /locus_tag="PK264_22305"
     CDS             complement(4784..>5023)
                     /locus_tag="PK264_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78636.1"
                     /translation="RPGEQPVAIAPQPVPTTSPPPAVTPVPPPKAPGPVAQSGSCADA
                     CRAQHEQCRIASKGDPRCGQMLTACLQRCISSKGR"
BASE COUNT          822 a         1570 c         1859 g          772 t
ORIGIN      
        1 cttgccgcga gcgtgatgcc ggcggcaacc cgaacggccg tcatgatctt tgcgaacagc
       61 gttccgaagg cgttgatcgc gtccttcacg cggattgccg tggtgccggg tagctcgcgg
      121 ccgagatcgc gcgcgatcgc ggcttccgtg acgagcgcgg tgtcggcggg tagcgtgatc
      181 gtggcgagca ggttgtgcgg tgcgccggag agcgtgttcg gcgagaacac catgacgaag
      241 tttatggcga ggctctccca cttgagctcg cggaagcttg cgatgcgtgc ggtcacgtcg
      301 cggccgagga tattgacggt gacgctgtcg ccgatcttca agccaaggtc tttggcgagc
      361 tcgccttcga acgagacgag cggctcgccc tggtagtcct tcggccacca ggcaccggcg
      421 gtcactttcg agccttctgg cacgtcctcg gcgtaggtga ggccgcgatc gccttgcagt
      481 acccagctcg ccttgggcga cgccttgacg gtctcggccg gacggtcgcc gagcttgacg
      541 agacggccgc gcagcatcgg cgcatgacgg acttcagccg ccgcgacgtg gcgcttcacg
      601 atggcgtcgt ggcgatcgcg gtcgtcgcgg gggaggtcga ggatgaagta gttgggcgag
      661 cctttcggga tgcgcgcagt gagctcgtcg cggatcgaag cgtcggcaag cgacagcgtg
      721 acgaggaggg agaggcccga gccgagcgcc agcaccacgg agcgcgtgag gccgcccggc
      781 gcgccgaggc tgccgagggc gagcgagagc tccggtcggc gcgggcgtgg cagcgtgcga
      841 gcgaccagcg tgacgagctg gcccagtccg aggaagacgg cgaacacgcc agcgatgccg
      901 agcaggaaga agctcgcgat ccgccgatcc tccgacgaga ggatggcgag cacggcgagc
      961 aacgctgcga tcacgatgag tgatgcaatg ctcgtccagg gcagggaggt gcgggccggc
     1021 tgcacatcgt cgcggaagag cgaggcggcg cggatgcgcc cggcgcggcc gagcggccag
     1081 acagtgaaga ggagcgtcac cagtagcccg taggcgatcg ccgtcagcac gctgcgggcg
     1141 gagatcacga actcgccgcg gatcggcagc gcggctccga tcgttgcatc gagcgccagc
     1201 ggcataagca tgccgaggcc gaggccgatg ccgataccga ggagcccgat gatgaggatc
     1261 tgcacgagat gcagcgccgt gatcgtgcgc tggcgcgcgc cgagcgcctt cagagtcgcg
     1321 atcaccttgc ggcggcggtc catgtaggtc gcaacggcac tcgcgacgcc gacgccgccg
     1381 acgatgagcg aggtgaggcc gacgagggtg aggaactggc ggagccgctc gagcgtccgt
     1441 gagacggagg gcgagggatc gcggcgatcg gcaatgatga agccggattc ggggagatct
     1501 gccttcgcct tgtctcggaa gcggacaagg tcggcattgt cggctgtggg gccagcggcg
     1561 gcgagcttca gcgcgtagcg ccagcgcacg agactgccgg gctcggcgag ttgcgaggcc
     1621 ttcagcgtat cgagtgagac gaggacgcgt gggccatagg tcagccggtc ggcgatcttg
     1681 tccggctcgg cgacgatggt ggcgcggacc tcgacctctg tgcggccgag cttgatgcga
     1741 tcaccgagct tgatgccgag ccggtcgagc agaattggct cgacggcggc gccgcggccg
     1801 gccagcgccg tgctcgcggt gacgccggag gacagggtga gtgtgccgga aagcgggtag
     1861 agatcgtcga tcgccttgag ctcgatcagt gcctgctcgt ctgcgcccgc ggctggggcg
     1921 gcggtgcggg ccatggagcg cagcgtcgcc gcctcgctga ctttgccgtg ggtcgcaatc
     1981 cagcggcgct cggcctcggt cgcagccttg tgcgagcggg cgagagtgac gtcgccgccg
     2041 agcagtgctt cgccctgccg ttcgaacccc gctcggagcg catcggagag tgcgccgacg
     2101 cccgcgatca cggcgacgcc gagcgcgata caggcgagga aaacgtagaa gcccgagaga
     2161 cctgagcgga gctcgcggag cgcgagcgtc agcacaacag gccaacgcca tccagtggcc
     2221 cgcgtggtga gcccttcagt tgcaatggtc atgcgagcgc accggtagcg acccgtgaga
     2281 cgatgcgacc gtcggccatg tggactgtcc ggccgcagcg ccgcgccagc gcgtgatcat
     2341 gcgtgatgag gacgagtgtc gtccgccgcc gcttctgcag gtcgacgagc aggccgacga
     2401 cggtctcgcc ggtctgccca tcgagattgc ccgtcggctc gtcggcgagg agcagcttcg
     2461 gccgcgtcac aagggcgcgg gcgatggcga cacgttgttg ctcccccccc gagagctcgg
     2521 ccgggaaatg atcgacgcga tggccgagcc cgacctcgcc gaggagcacg cgcgcctcga
     2581 ccaatgggac gatatcgcgg cggccggcaa gctcgagcgg gagcgccaca ttctcgagcg
     2641 cggtcatggt cggcacgagg tggaacgact ggaagacgat gccgatcgcg gcgccgcgga
     2701 caagggcgag ctggtcctcg ccgagcgccg agaagtcctg gccgacgacg ctgacactcc
     2761 cgcgcgttgc ccgttcgagg ccggcaatga tcatgaggag ggaagtcttg cctgagccgc
     2821 tcggtccgac gacggcgacc gtctcgcctt cgccgatgtc cagggagaca ttgcgcagga
     2881 tgtcgaccga gcccgcgcgg ctcgtcagcg aaagtgagac gttggcgagc gagacgacgg
     2941 cgcgggcgcc tgcaccgata gcgccgtgct cgctcgcagg ggtggcgtct tgcatttcat
     3001 gtcctcatgg cgccgggcga tgaagccacc cgctgccgtc agatggtggt cgaaattgga
     3061 cccggcaagg cacgctgacc tgcgcatctt acgcacaccg aggcactccc gatctgccgg
     3121 gcaggggctt caggccgttc gcaacggata gatgagcgag aacgccatgg tctcgttgga
     3181 aatgaccgat acatcgagcg gcgtcacatc gagcggcggc atcagcttgg cgggcaacaa
     3241 ggtgccaccg tcccagagag cgacaagacg tgtgccgcgg agctgcgcaa ggctctcgat
     3301 gagcctcgcg agcgcggcga gcatcgccgt gtgcttgcgg tcctgatcga cgtcgtcggg
     3361 ttcgcccggg tcgaagtatg ccttgaaatc gtcgagtgcg ctgcagcagc aaccgtccca
     3421 ctccgtcgtc acgcggacag agtgggcagt gccgatgacc gcgtgcacgt ccggccggac
     3481 gtcggcaacg aggccgacgt caccgaacgc tgtcgcgata gcctgcacct cgatcggccg
     3541 ctcgctgccg aagtagagaa cgaggcacat gcggtctcag ctcgcgatca gcacgtcggt
     3601 atagagttcg ctcggatcgg gctcgggatc ggactgcgag aactcggctg ctacggtaac
     3661 gatttggcgg atctggccgt cgatcgcctt cagctcgtcc tcgctcgcga ggcgcccgtc
     3721 gagcagccgc tttctcacgc gctcgatcgg gtcgcgctcc tcgcggacgc gctcgacctc
     3781 ctcgcgggta cggtacttgg ccgggtccga catggagtgg ccgcggtagc gataggtcat
     3841 catctcgagg atgtaggggc cgttgccagc gcgggcatgg gcgacgacct tgtcgccggc
     3901 ggccttcaca gcgcgcacgt ccatgccgtc gacctgctcg ccggggatgt cgaatgagga
     3961 gccgcgcagc gagaagttgg tcgtggcggc cgcacgctcg atcgcggtgc ccatggcgta
     4021 gcggttgttc tcgatgatga agatcaccgg cagcgaccag agcttggcga tgttgaagct
     4081 ctcgtagacc tggccctggt tggcggcgcc gtcgccgaaa taggtgaggc atacgctgtc
     4141 gttgccgcga tatttgttgg caaaggcgag gccggcgccg aggggcacgt tggcgccgac
     4201 gatgccgtgg ccgccgtaga agttcttctc cttggagaac atgtgcatcg acccgccctt
     4261 gccgcgcgag tagccgctgc gccggcccgt cagctcggcc atgacgccct tcgggtccat
     4321 gccgcaggcg agcatgtggc cgtgatcgcg gtaggttgtg atcacctgat cgctcgggcg
     4381 aatcgccatc tgcatgccga cgacgacggc ctcctggccg atgtagagat ggcagaagcc
     4441 gccgatcagc cccatgccgt agagttggcc ggcgcgctcc tcgaagcggc ggatgagcag
     4501 catctcgcgg taggcggcga gctcctgctc ccgcgtgaag gccggcacca tactgcgcac
     4561 gccggtgccg ggagtgccga ggaagccgga ggtggcgtca ggggctgcga gcgcggggct
     4621 cgacgcgtcg ggtctgccgg cgaagggaaa cgatgctgcc acggccatga tcatcctcgt
     4681 tgcgatgtgg cggcagacta tcgacctcgg acgagaaaag caaaacgggg ccgcggatcg
     4741 cgcaccccgc tcatgtgtcg atgtggcgag tcggctccgg gcctcagcgc cccttgctgc
     4801 tgatgcagcg ctgcaggcag gccgtcagca tctggccgca acgcggatcg cctttcgagg
     4861 cgatgcggca ctgctcatgc tgggcgcggc aggcatccgc gcacgacccg gattgcgcga
     4921 cggggcccgg ggccttgggc ggcgggactg gggtcacggc cgggggcggc gatgtcgtcg
     4981 gcacgggctg cggcgcaatg gcgaccggct gctcgcccgg gcg
//
LOCUS       DAORNH010000324         4992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12516_length_4992_cov_2.803119, whole genome shotgun sequence.
ACCESSION   DAORNH010000324 DAORNH010000000
VERSION     DAORNH010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12516_length_4992_cov_2.803119"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /gene="rpoD"
                     /locus_tag="PK264_22310"
     CDS             complement(<1..1717)
                     /gene="rpoD"
                     /locus_tag="PK264_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRD78637.1"
                     /translation="MATSTAKSEEQTAADGAPTERDSPLLDMSDQSVKKLLKLGKTRG
                     YVTYDELNAVLPSEEVSSEQIEDTMAALNDMGINVVETEEGEEAQPAADAADDSADEE
                     SRAVVAQSVPVRAETRSEAGDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREA
                     MIAGLCESPLTFQAIIIWREELDQGKLLLRDIIDLEATYEGPEAKAAPPPLVMPGAGV
                     PPPAAAEPAEDGEEGDATAPPPGEMDDEDDYENALSLAAMEAELKPKVLETFDRIAGT
                     YKKLRKQQDKKLEQQVAGEQGSRQLQKAYDKLREEIIADVKSLSLSNNRIELLVEQLY
                     SINKRLVSFEGRLMRLADSYGVAREDFIGEYYGNELDQTWLERMGKFGKKSWVNFLKH
                     ERRQVETIRGQIHELASETGLEIAEFRRIVKHVQKGEREARQAKTEMVEANLRLVISI
                     AKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQAR
                     TIRIPVHMIETINKIVRTSRQMLHEAGREPTPEELAEKLAMPIEKVRKVLKIAKEPIS
                     LETPIG"
     gene            complement(2115..4064)
                     /gene="dnaG"
                     /locus_tag="PK264_22315"
     CDS             complement(2115..4064)
                     /gene="dnaG"
                     /locus_tag="PK264_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRD78638.1"
                     /translation="MRYPPSILDEIRARLPVSQVVARKVALKKAGREFKGLSPFKTER
                     TPSFFVNDQKGFYHCFASGEHGDIFSFVMKTEGLSFMEAIERLAGEAGVPLPKPAERD
                     QRREDERTRLYSLLEASAKFFEERLSGLDGAEARRYIEKRGLARHTLAEFRIGFAPPG
                     RSVLKAHLAKAGYAVEEMIQSGMLIGGEDIAEPYDRFRNRVMIPIADAKGRIIAFGGR
                     ALDPAAPAKYLNSPETPLFHKGSVLFNAHRARTAAFEKSRIIAVEGYLDVIAFHEAGI
                     KECVAPLGTALTEEQVGLLWRLSPEPILCFDGDSAGRKAAWRAIDVALPHLKPGASLR
                     FAFLPDGLDPDDLVRQSGADAARAILETTRPLADVLWEREWSSGDWSTPERRAGLERQ
                     LAGHLARIADGAVRGHYDADMRGRLAKAWGQGRTYPARPGQLSHRATGSTSGFRPKFG
                     QGRPGGQGFPNQPPPGASSSLRNSRLVQGGLQAPPYREALLLRTLINHPWLLDEDAER
                     IAELPMTSAVTERLRDALLSIHAANNPLDRTTLASQLTALGLGKVLDLVERSITHRSD
                     KFSEPDADRAAVEAGWHHATAIHDRQVGLRRALAAAEQAWLTDASAEALDRITALQQQ
                     LALTERSEDAGSEGTLGARRDGAMA"
     gene            complement(4389..4961)
                     /locus_tag="PK264_22320"
     CDS             complement(4389..4961)
                     /locus_tag="PK264_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321192.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HRD78639.1"
                     /translation="MADAQPISRFPVPDVKSLPADVRERIEKVQEKSGFIPNVFLVLA
                     HRPDEFRAFMAYHDALMDKPGGLTKAEREMIVVATSAANQCLYCVMAHGAILRIRAKS
                     PLVADQVAVNYRKADITPKQRRMLDFAMKVALASAEVSDADIAAMKEAGFAEADIWDI
                     GAIAAFFAMSNRMANLTSMRPNTEFFAMGR"
BASE COUNT          747 a         1611 c         1706 g          928 t
ORIGIN      
        1 cgccgatcgg cgtctcgagg ctgatcggct ccttggcgat cttcaggacc ttgcggacct
       61 tctcgatcgg catggcgagc ttctcggcga gttcctcggg cgtcggctcg cggccggcct
      121 cgtgcagcat ctgccggctg gtgcgcacga tcttgttgat cgtctcgatc atgtgcacgg
      181 ggatgcggat ggtgcgggcc tggtcggcga tcgagcgcgt gatcgcctgg cggatccacc
      241 acgtcgcata ggtcgagaac ttgtagccgc ggcggtactc gaacttgtcg accgccttca
      301 tgaggccgat gttgccttcc tggatgaggt ccaggaactg caggccgcgg ttcgtgtact
      361 tcttggcgat cgagatcacg aggcggaggt tcgcctccac catctcggtt ttcgcctggc
      421 gcgcctcgcg ctcgcccttc tggacgtgct ttacgatgcg gcggaactcg gcgatttcga
      481 ggcccgtctc gctcgcgagc tcgtggatct ggccgcggat cgtctcgacc tggcgccgct
      541 cgtgcttcag gaagttgacc cagcttttct tgccgaactt gcccatgcgc tcgagccacg
      601 tctggtcgag ctcattgccg tagtactcgc cgatgaagtc ctcgcgcgcg acgccgtagc
      661 tgtcggcgag gcgcatcagg cggccttcga agctgacgag gcgcttgttg atcgagtaga
      721 gctgctcgac cagcagctcg atgcggttat tggagagcga caggctcttc acgtccgcga
      781 tgatctcctc gcggagcttg tcataggcct tctggagctg gcgtgagccc tgttcgcccg
      841 cgacctgctg ctcgagcttc ttgtcctgct gcttcctgag cttcttgtaa gttcccgcga
      901 tgcggtcgaa ggtctcgaga accttaggct tgagctcggc ctccatggcg gcgagcgaga
      961 gggcgttctc gtaatcgtcc tcgtcgtcca tctcgcccgg cgggggcgcg gtggcgtcac
     1021 cctcctcgcc gtcctcggcg ggttccgccg cggcaggcgg cggcacgccc gcgcccggca
     1081 tgacgagcgg cggaggggct gccttcgcct cggggccttc gtaggtcgcc tcgaggtcga
     1141 tgatgtcacg cagcagcagc ttcccctggt cgagctcctc gcgccagatg atgatcgcct
     1201 ggaaggtgag cgggctctcg cagagacccg cgatcatcgc ctcgcggccg gcctcgatgc
     1261 gcttggcgat ggcgatctcg ccctcgcggg acaggagctc gaccgagccc atctcgcgca
     1321 ggtacatgcg gacgggatcg tcggtgcggt caccggcctc gctgcgggtc tcggcgcgca
     1381 cgggtacgct ctgggcgacg accgcgcggg actcctcgtc ggcggaatcg tcggcggcgt
     1441 cggcggccgg ctgtgcctcc tcgccctcct ccgtctcgac gacgttgatg cccatgtcgt
     1501 tcagcgccgc catggtgtcc tcgatctgct ccgaggagac ctcctcggag gggaggacag
     1561 cattgagctc gtcgtaggtc acatagccgc gcgtcttgcc gagcttcagg agctttttga
     1621 ccgactgatc ggacatatcg agcagcgggc tgtcgcgctc tgtcggcgcg ccgtcggctg
     1681 cagtctgctc ctcggatttc gcggtgctgg tggccatgca agtctccagg gagaaggatg
     1741 atgatgcgct aagcgcccaa tgcggaccga gggcctcggc cggctcgacg aggtgaactg
     1801 aatgctgatc tagcgttcga tggaactcga tgtcggcgga cgctttcgag cagcagtcgc
     1861 cgagcccggc gggtcggcga tggtgtcagc acgatccggt gcgatgctca ttgctgtcat
     1921 ctgggctgtc ccctggcttg ccattcgacg gttccggctt gcgctgtcgc cgggacgcgg
     1981 cagctcgacc agacgcgcgt gctgcaaccg aaccacagtc ataggcacca tcgtgaacgt
     2041 catcggcctg gcacacgtcg gagttgaccg gcagctggcc gtggaacgct cacgtctcgg
     2101 ccaaattccc ccggtcatgc catggccccg tcgcggcggg cgccaagcgt accttccgaa
     2161 cctgcatcct cgctccgctc ggtcaaggcc agctgctgct gaagcgcagt gatccgatcg
     2221 agtgcctcgg cactcgcatc tgtcagccat gcctgctctg cggcggcgag agcgcgtcgc
     2281 agtccgacct gccgatcgtg gatggccgtg gcatgatgcc agccggcttc cactgccgcg
     2341 cgatccgcgt ccggctccga aaatttgtcg gagcgatggg tgatcgagcg ctcgacgaga
     2401 tcgaggactt tgcccaagcc tagagcggtc aattgggaag ctagcgtggt cctgtcaagt
     2461 ggattgttgg cagcgtggat ggaaaggagc gcgtcccgca gccgttcggt cacagccgag
     2521 gtcatcggca gctcggcgat ccgctcggca tcctcgtcga ggagccaagg gtgattgatc
     2581 aatgttcgca acagcaaggc ctcgcggtaa ggcggtgcct ggagcccgcc ctgaaccagg
     2641 cggctgttgc gcagactcga ggatgctccc ggcggaggtt ggttcgggaa gccctgcccc
     2701 cccgggcgtc cctgcccgaa tttcggccgg aatccgcttg tgctgcccgt ggcgcgatgg
     2761 gacagctgtc cgggtcgagc ggggtacgtg cggccctggc cccaggcctt ggcgaggcgg
     2821 ccgcgcatgt ctgcgtcgta atggccgcgc acggcgccgt cggcgatacg cgcgagatga
     2881 cccgcaagct gccgctcgag gcccgcgcgc cgctcaggcg tcgaccagtc gcccgacgac
     2941 cactcccgtt cccagaggac gtcggcaagt ggtcgcgtcg tctcgaggat ggcacgagcg
     3001 gcgtcggcgc cgctctggcg gacgaggtcg tccggatcga ggccgtccgg gaggaacgca
     3061 aagcgcaggc tcgcgccggg cttcagatgc gggagagcca cgtcgatcgc ccgccaggcg
     3121 gccttccggc ccgcgctgtc gccgtcgaag cacaggatcg gctcgggcga gagacgccag
     3181 aggagaccga cctgctcttc cgtcaatgcc gtcccgagcg gtgccacgca ctccttgatg
     3241 cccgcctcgt ggaaggcgat cacgtcgaga tagccttcga ccgcgatgat gcggctcttc
     3301 tcgaaggcgg ccgtacgcgc gcggtgcgca ttgaagagga cgctcccctt gtggaagagg
     3361 ggcgtctcgg gcgagttcag gtacttggcc ggggccgcgg gatcgagcgc gcgcccgccg
     3421 aaggcgatga tgcggccctt ggcgtcggcg atggggatca tgacgcgatt gcggaaacgg
     3481 tcgtagggct ccgcgatgtc ctcgccgccg atcagcatgc cgctctggat catctcctcg
     3541 accgcatatc cggccttggc gagatgagcc ttgaggaccg agcggccagg aggcgcgaag
     3601 ccgatcctga actcggcgag cgtatggcgg gcgaggccgc gcttctcgat gtagcggcgg
     3661 gcctctgcgc catcgaggcc cgagagccgt tcctcgaaga acttggccga ggcctcgaga
     3721 agcgaataga gccgcgtgcg ctcgtcctcg cgccgctggt cgcgctcagc gggcttcggc
     3781 aacggcacac cggcttcgcc cgccagacgc tcgatcgcct ccatgaacga gagaccttcc
     3841 gtcttcatga cgaaggaaaa gatgtcgccg tgctcgcccg atgcgaagca gtggtagaag
     3901 cctttctggt cgttcacgaa gaacgacgga gtcctctcgg tcttgaaggg cgagagcccc
     3961 ttgaactcgc gacccgcctt cttcagcgcc accttgcgcg cgacgacctg gctcaccggg
     4021 aggcgagcgc ggatctcgtc gagaatggac ggcgggtagc gcatcgggct ccggttcggt
     4081 gtggcgcccc gctcgatcga atcggggcct tgtccgattc gacatatagg cgattctcga
     4141 tgcccacgcg ccggagcaac gtatcgtccc cgctttcccc aggtggcgcc ccattatcga
     4201 tcgacctgcc gacgggcagg cggcggcacc gatcatttcc cggcaaagat gaccgcgtcc
     4261 acacgcgcct tccttgcgac tggacggggc cggccaccaa agcgtgaaca gagtgccggc
     4321 atgacgcttg gggtggtatg gcgtttgccg ctctgccacc gccccgacac cagcgctgac
     4381 ctccgggctc accggcccat ggcgaagaac tcggtgttag ggcgcatgct cgtcaggttg
     4441 gccatgcggt tcgacatggc gaagaaggcg gcgatggcgc cgatgtccca aatgtcggcc
     4501 tcggcgaagc ccgcctcctt catggcggcg atgtcggcgt ccgagacttc ggccgaggcg
     4561 agagccacct tcatggcgaa gtctagcatg cggcgctgct tcggcgtgat gtcggctttc
     4621 cggtagttga cggcgacctg gtcggcaacc aacgggctct tggcgcggat gcgcaggatg
     4681 gcgccgtggg ccatcacgca gtagaggcac tggttggcgg cgctggtggc gacgacgatc
     4741 atctcgcgtt cggccttggt gaggccgccg ggcttgtcca tcagcgcatc gtgataggcc
     4801 ataaaggcgc ggaactcgtc cggacggtgg gcgagcacga ggaacacgtt ggggatgaag
     4861 ccagacttct cctgcacctt ctcgatgcgc tcgcggacat ccgcgggcag gcttttgaca
     4921 tcgggaaccg ggaagcggct gattggttgc gcgtccgcca tagcgggctc tccatcgtta
     4981 ggactggctc tc
//
LOCUS       DAORNH010000325         4933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12720_length_4933_cov_2.915129, whole genome shotgun sequence.
ACCESSION   DAORNH010000325 DAORNH010000000
VERSION     DAORNH010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12720_length_4933_cov_2.915129"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>2386
                     /locus_tag="PK264_22325"
     CDS             13..>2386
                     /locus_tag="PK264_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD78640.1"
                     /translation="MAWLAYGLLVLAIAAVLTIYGVFTAVRLPVDVFPDLNRPTVTIM
                     TEAEGLAPTEVEQLVTFPIETQMNGLPGVTRVRSVSGVGLSITYVEFDWGTDIYRNRQ
                     QIAERLARVQSQLPANVVPQMGPINSIMGQILMFALTSETASPMEVREIADFVVRPRL
                     LTIPGVAQVIPMGGEVRQYRVSPNTPAMRALGVSLADLERALQQFGTNTGGGFADQYA
                     REFLVRNVGRTLKLEDLRDVVVRTGTGGSVYLHQVADVSYGARLKRGEAGFMGAPAVI
                     ISVEKQPGIDTVKLSREVERAMAELNRTMPKGIKVDTVVFRQANFIETSIRNVEKVLL
                     EAIAVVAIVLFLFLLNTRTTLISLTAIPVSMLVTAIVFYAFGLSINTMTLGGLAIAIG
                     ELVDDAVVDVENIFRRLRENREKGNPRSTFDVVVSASQEVRSGIVYATMIIVLVFVPL
                     FALSGIEGRLFAPLGHAYIISILASLVVSITLTPVMAYYLLPSLKRLDEREGPLVRVL
                     KAANRRLLGWAFRHPRTLITGVLTAVVMAIGALVALPRSFLPPFNEGTLTINLLFQPG
                     ISLAESHRVGFIAEKLVMEVPEVKLVARRTGRAELDEHAEGVHSSEIEVDLKASARDK
                     VEIVNDIRARLAVLPVSTNVGQPISHRLDHMLSGVRAEIALKIFGEDLDTLIRLAEEM
                     RQRLAAIPGLADLQVERQVRIPQLDITVDYKKAALYGVQPAHVTEQLERLSNGRLVSR
                     LIDGNRRFDVVVRLDDRLRTTERLAELLIETPRGWVPLRQIADVKETDGPN"
     assembly_gap    2387..2484
                     /estimated_length=98
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2485..3144
                     /locus_tag="PK264_22330"
     CDS             <2485..3144
                     /locus_tag="PK264_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRD78641.1"
                     /translation="EIAGARLPTGYFTSLEGTFQAQEESMRTIGLLSLVSLAMVFTIL
                     YSRYRSTLFALIVMGSVPLALIGSVIALWSAGQPLSVASMIGFITLTGIATRNGILKI
                     SHTINLAIREGMPFGPDLVMRGSLERMTPVLMTALSAGVALIPLIIDAGAPGKEILHP
                     VAITIFGGLVSATALDALLTPVLFLRYGRKSLERLVADARAKTQSGETLPRGAETADA
                     Y"
     gene            3165..3545
                     /locus_tag="PK264_22335"
     CDS             3165..3545
                     /locus_tag="PK264_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78642.1"
                     /translation="MKPARILISFALVLGSMLASPLLAHEPAKGRNGGLRVDAGVYHT
                     ELLVDGTTKVVIFLSDVEDKPIPSAGFKGTAILIIDGKSQRIEMLPAEASMLVGTAPA
                     PVQPGVKGVVQLFSPDGKTSQGKF"
     gene            complement(3634..4275)
                     /locus_tag="PK264_22340"
     CDS             complement(3634..4275)
                     /locus_tag="PK264_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HRD78643.1"
                     /translation="MMLVLHGYEFSVYNRIVRIALIEKGLSYSRVEVNPFSEDCPPSY
                     IALNPFRRVPTLVHGDFVLYETSAITRYLDETFPTPPLLPIEPCARARVAQVISIIDG
                     YGYWPLVRQVFSHRVFRPLTGKSADEAEVQAGLANAGRVLAALEGLARGGETLVGDGW
                     TMADGHLAAMVDYFTMAPEGAAMLADYPRLAEWWRASSARPSVIATRPAFPQY"
     gene            complement(4272..>4933)
                     /locus_tag="PK264_22345"
     CDS             complement(4272..>4933)
                     /locus_tag="PK264_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRD78644.1"
                     /translation="EELARSAEAEGQSVNWVAVNGKLAGLIAVADTLKAGAGEVIGRL
                     RERGIEVVMATGDAAPAANAVAAALGISSVHATMLPSEKAKLVQSLKAAGRRVAMAGD
                     GINDAPALAVADVGIAMGTGADIAKDSAGLTLVKGDVAGLERALALAVATANNMRQNL
                     ALAFGYNALAIPVAAGVLFPFIGMMLSPMLAAAAMSLSSVSVIANALRLGAANLERKT
                     A"
BASE COUNT          790 a         1618 c         1577 g          850 t
ORIGIN      
        1 tggcggcatc cgctggcctg gctcgcctac ggcctgctcg tcctggcgat cgccgcggtt
       61 ctcacgatct atggcgtgtt cacggcggtg cgcctgccgg tcgacgtttt cccggacctc
      121 aacaggccga ccgtcaccat catgacggag gcggaaggac tggcgccgac cgaggtcgag
      181 caactcgtca cattcccgat cgagacgcag atgaacgggc ttccgggcgt gacgcgcgtg
      241 cgttcggttt ccggcgtcgg gctctcgatc acctacgtcg agttcgactg gggaacggac
      301 atctaccgca accgccagca gatcgccgag cgtctcgcgc gggtgcagtc gcagttgccg
      361 gccaacgtcg tgccgcagat gggcccgatc aactcgatca tgggccagat cctcatgttc
      421 gcgctgacga gcgagacggc gagcccgatg gaggtgcgag agatcgccga tttcgtcgtc
      481 cggccgcgac tgctgacgat cccgggtgtc gcccaggtga tcccgatggg cggcgaggtg
      541 cgccagtatc gcgtctcgcc gaacactccg gcgatgcggg cactcggcgt gtcgctggca
      601 gatctcgagc gggcgctcca gcagttcggc accaacacgg gtggcggctt cgccgaccag
      661 tacgcccgcg agttcctggt tcgcaacgtc ggccgcacac tgaagctcga ggacctgcgc
      721 gatgtcgtcg tgcggaccgg gacgggggga tcggtctacc tgcatcaggt ggcggatgtg
      781 agctatggcg cgcggttgaa gcggggcgaa gctggcttca tgggcgcgcc cgcggtcatc
      841 atctcggtcg agaagcagcc gggcatcgac accgtcaagc tctcccgcga agtcgagcga
      901 gcgatggccg agctcaatcg caccatgccg aagggcatca aggtcgacac cgtcgtcttc
      961 cgccaggcga acttcatcga gacctcgatc cgcaatgtcg agaaggtctt gctcgaagcc
     1021 atcgccgtgg tggcgatcgt gctgttcctc ttccttctca acacgcgcac gacgctgatc
     1081 tcgctgaccg cgatccccgt ctccatgctg gtgacggcga tcgtgttcta tgccttcggc
     1141 ctctcgatca atacgatgac gctcggtggg ctcgccatcg ccatcggtga gctcgtcgac
     1201 gatgccgtgg tcgatgtcga gaacatcttc cggcgcctcc gcgagaaccg cgaaaagggc
     1261 aatccgcgct cgacgttcga tgtcgtcgtc tcggcgagcc aggaggtgcg ctccggcatc
     1321 gtctacgcga ccatgatcat cgtgttggtg ttcgtgccgt tgttcgcgct ttcgggcatc
     1381 gagggccgtc tctttgcccc gctcggccac gcctacatca tctcgatcct ggcgagcctc
     1441 gtcgtttcga tcacgctcac cccggtgatg gcctactacc tgctgccctc gctgaagcgg
     1501 ctcgacgagc gcgaggggcc actcgtacgc gtcctgaagg ccgccaatcg ccggctcctc
     1561 ggctgggcgt tccgccatcc ccgcacgctg atcacgggcg tgctgacggc ggtcgtgatg
     1621 gcgatcggtg cgctcgtcgc gttgccgcgc tcgttcctgc cgcccttcaa cgagggtacg
     1681 ctcacgatca atctcctctt ccagccgggc atctcgctcg ccgagagcca ccgcgtcggc
     1741 ttcatcgccg agaagctcgt gatggaggtg ccagaggtga agctcgtcgc gcggcgcacg
     1801 gggcgcgccg aactcgacga gcatgccgag ggcgtccact cctccgagat cgaggtcgat
     1861 ctcaaggcgt cggcacgcga caaggtcgag atcgtcaacg acattcgcgc gcgccttgcc
     1921 gtgctgccgg tctcgaccaa cgtcggccag ccgatctcgc atcgcctcga tcacatgctc
     1981 tcgggtgtca gggccgagat cgcgctcaag atcttcggcg aggacctcga cacgctgatc
     2041 cgcctcgccg aggagatgcg ccagcgcctc gcggcgatcc cgggtctcgc cgatctccag
     2101 gtggagcggc aggtgcgcat tccccagctc gacatcaccg tcgactacaa gaaggcggcg
     2161 ctctacggcg tgcaaccggc gcacgtcacc gagcagctgg agcgtctttc gaacggtcga
     2221 ctcgtctcgc gcctcatcga cggcaaccgg cgcttcgacg tcgtcgtgcg tctcgacgac
     2281 cggctgcgca cgaccgagcg gcttgccgag ctcctgatcg agacgccacg ggggtgggtg
     2341 ccgctccgcc agatcgccga cgtgaaagaa accgacgggc ccaaccnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnngagatc gcgggcgcgc gcctgccgac cggctacttc
     2521 acgagcctcg aaggcacctt ccaggcgcag gaggagagca tgcgcacgat cgggctgctc
     2581 tcgctcgtgt cgctcgccat ggtgttcacg atcctctaca gccgctaccg ctcgacgttg
     2641 tttgctctca tcgtcatggg gagcgtgccg ctggccctga tcggctcggt gatcgcgctc
     2701 tggtctgcgg gccagccgct ctcggtcgca agcatgatcg gcttcatcac gctgaccggc
     2761 atcgccacgc gcaacggcat cctgaagatc agccacacca tcaatctcgc catccgggag
     2821 ggcatgccgt tcggccccga tctcgtgatg cgcggtagcc tcgagcgcat gacgcccgtg
     2881 ctcatgacgg cgctctcggc gggcgtcgcg ttgatcccgc tgataatcga tgctggcgca
     2941 ccggggaagg agatcctgca tccggtcgcg atcacgatct tcggcggcct cgtcagcgcg
     3001 acggcgctcg acgcgctcct cacaccggtc ttgtttctgc gctacgggcg gaaatcgctc
     3061 gagcggcttg tcgccgacgc gcgggccaag acccagtcag gcgagacgct gccgcgcggc
     3121 gccgagacgg ctgacgccta ctgatcacca accaggagaa acccatgaaa cccgcaagaa
     3181 ttctcatatc cttcgccttg gtgctcggct cgatgctggc ctccccactt cttgctcatg
     3241 agccggcgaa ggggcgcaac gggggcttgc gcgtcgatgc cggtgtctat cacacggagc
     3301 tcctcgtcga cggcacgacg aaggtcgtga tcttcctctc cgacgtcgag gacaagccga
     3361 tcccgtcggc gggcttcaag ggcaccgcca ttctgatcat cgacgggaaa tcgcagcgca
     3421 tcgagatgct gccggcggag gcttcgatgc tggtcggcac ggcgcccgcg ccagtgcagc
     3481 ccggcgtcaa aggcgtcgtg caactcttct cccccgacgg caagacgtcg cagggcaagt
     3541 tctagaattg gatggtggcg gggcgtcgcc ccccacccgg gggaggcagc gatctctatt
     3601 cgcacccgat ccgatggctc acatcagatc gcatcagtac tgtggaaagg cgggcctcgt
     3661 tgcgatgacg cttgggcgtg ctgacgacgc tcgccaccat tcggcgaggc gcggatagtc
     3721 ggcaagcatc gcggcaccct ctggcgccat cgtgaagtag tcgaccatgg cggcgagatg
     3781 accgtcggcc atcgtccaac catcgccaac gagggtctcg ccgccacgcg ccaggccctc
     3841 gagtgccgcg agcacgcggc cggcgttcgc gaggcctgcc tgcacctcgg cctcgtcggc
     3901 agacttgccc gtcagcgggc ggaagaccct gtgcgagaag acctgacgta ccagcggcca
     3961 atagccgtag ccgtcgatga tggagatgac ctgtgcgacg cgcgctctcg cacagggctc
     4021 gatcggcagg agcggcggcg tcgggaaggt ctcgtcgaga tagcgcgtga tggcagatgt
     4081 ctcgtagagc acgaagtcgc cgtgaacgag tgtcggtaca cggcgaaagg gattgagcgc
     4141 gatgtagctc ggtgggcaat cctcactgaa ggggttcacc tcgacccgcg agtatgagag
     4201 ccctttttcg atcagcgcga tccggacaat gcgattgtag acgctgaact cgtagccatg
     4261 gagcacgagc atcatgcggt tttccgttcc aggttggcgg cgccaagtcg cagcgcgttg
     4321 gcgatcaccg aaacggacga gaggctcatg gccgcggcgg cgagcatcgg agagagcatc
     4381 atgccgatga aggggaagag cacgccggcg gcgaccggaa tggcgagagc gttgtagccg
     4441 aacgcgagcg cgaggttctg gcgcatgttg ttcgcggtcg cgacggcgag ggccagcgcc
     4501 cgttcgaggc cggcgacatc gcctttgacg agtgtcagac cggcgctgtc cttggcgatg
     4561 tcggcgccgg tgcccatggc gataccgacg tcggcgacgg cgagtgccgg cgcatcgttg
     4621 atgccgtcgc cggccatggc gacccggcgg cccgcagcct tcagcgactg cacgagcttc
     4681 gccttctccg atggcagcat ggtggcgtgc acgctcgaaa tgccgagggc tgccgcaacc
     4741 gcatttgcag ccggtgccgc atcgcctgtc gccatgacga cctcgatgcc ccgctcccgg
     4801 agtcggccga taacctcgcc cgcgcccgcc ttcaacgtat cggcaaccgc aatgagcccg
     4861 gcgagtttgc cgttcacggc gacccagttg acgctctggc cttccgcctc ggcgctcctt
     4921 gcaagctcct cga
//
LOCUS       DAORNH010000326         4932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12724_length_4932_cov_2.615747, whole genome shotgun sequence.
ACCESSION   DAORNH010000326 DAORNH010000000
VERSION     DAORNH010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12724_length_4932_cov_2.615747"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>887)
                     /locus_tag="PK264_22350"
     CDS             complement(<1..>887)
                     /locus_tag="PK264_22350"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78645.1"
                     /translation="KLEDDLIRAVKETGVRVIGPNCQGALSVPSRMWCVFGSVSHETE
                     LKSGPVSCAFQSGGFGYAVVNLAEAQGIGFRNVVSTGNETDITMPELLSAFLDDEGTK
                     LAFAYLEGTPDARRFLEVGRKSLELGKPIILWKGAQTESGTKAAASHTANMTGNYDLY
                     RAAFRQAGIIEVQDVEEIVDIAKVAGQGRLPKGRAIGVLSISGGSGIVYADRAVKEGL
                     TLPNFSQSTVAALKKIIPSFGSSENPADTTAGVFNDPALFTKTLEIVLADPALDQVSI
                     LLASISGEPALRACQAIAD"
     assembly_gap    888..905
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<906..1230)
                     /locus_tag="PK264_22355"
     CDS             complement(<906..1230)
                     /locus_tag="PK264_22355"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRD78646.1"
                     /translation="MATSPFARLFSPNAIAVVGASADLARISGQPIAALKNSGFKGPV
                     HLVNPKYGELHGLKCYPSVAAIGSPVDLALIAVPAAGVPAAVRECGAAKIPFAIILTA
                     GFREAG"
     assembly_gap    1280..1289
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1337..1681)
                     /locus_tag="PK264_22360"
     CDS             complement(1337..1681)
                     /locus_tag="PK264_22360"
                     /inference="COORDINATES: protein motif:HMM:NF012254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HRD78647.1"
                     /translation="MAHGRFSRLLNATFAGLALAAAAGSAAAQSPSPAAPPATLTAAA
                     TMERLKGMWIEGPGFDITYGAAYEACATRCLANARCRMIEYYRPERKCNLYDTVRPRL
                     KGGSSDVAIRRP"
     gene            complement(2026..2364)
                     /locus_tag="PK264_22365"
     CDS             complement(2026..2364)
                     /locus_tag="PK264_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HRD78648.1"
                     /translation="MTRYLISFDDGAMVIPEQELPAVSEAAHAVVRAAQEAGVWIFGG
                     GVASQRASIVAADGTVTDGPYPETKAVVGGFVIIDVPSREVALEWAARFAAACRCAQE
                     VREIMFDPLV"
     gene            complement(2606..3031)
                     /locus_tag="PK264_22370"
     CDS             complement(2606..3031)
                     /locus_tag="PK264_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HRD78649.1"
                     /translation="MSQRSWIAVASAEHVKIGRAQGFMQVCHGKAAPLRRLSLGHSIA
                     YYSPTEHFRGKEKLQAFTAIGIVASGAPYQADMGGGFCPFRRNVQWAEADAAPIRPLL
                     GRLAFTRDGQCWGYQLRFGLFEISGADMQIIAAAMGAAL"
     gene            complement(3028..3399)
                     /locus_tag="PK264_22375"
     CDS             complement(3028..3399)
                     /locus_tag="PK264_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD78650.1"
                     /translation="MPDFSILLLHVESPAVSAKFYNELFDIPIVEQSPTFAMLPLRDG
                     VMLGLWSRASVEPKTTGHPGSSEVAFTVADAAAVSETFTAWKKRGLAIAQTPTRMDFG
                     TTFVALDPDGHRLRVFVPEAA"
     gene            complement(3495..4170)
                     /locus_tag="PK264_22380"
                     /pseudo
     CDS             complement(3495..4170)
                     /locus_tag="PK264_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
     gene            complement(4242..>4932)
                     /locus_tag="PK264_22385"
     CDS             complement(4242..>4932)
                     /locus_tag="PK264_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78651.1"
                     /translation="STSATRRTTSSRRAPKPAAAAAGGAGKGLKPMPMSPGIMIGLLM
                     VAGVSCCVAMSMPQAHIVAYCGDLKYGAQHGADMLAVLLGFGVISRLVSGWVLDKVGG
                     FPTLIVGSALQAIALALYIPFDSLPALYLISAFFGIVQGGIVPSYAIVIREHFPPSEA
                     GAKFGLVLGSTIAGMALGGWVSGAIFDYTGSYKAAFVHGVAWNILNFAIVAWLMLRAR
                     ARRSDGALVAA"
BASE COUNT          874 a         1609 c         1610 g          811 t
ORIGIN      
        1 atcggcgatc gcctggcagg cgcgcagggc gggctcgccg ctgatcgagg cgagcaggat
       61 cgacacctgg tcgagtgccg gatcggcaag cacgatctcg agcgtcttcg tgaagagggc
      121 gggatcattg aagacgccgg ccgtcgtatc ggcagggttc tccgaggatc cgaagctcgg
      181 gatgatcttc ttcagtgcgg cgacagtgga ctgcgaaaaa ttcggcagcg tcagcccttc
      241 cttcacagcc cggtcggcat agacgatccc ggagccgccc gagatcgaga gcacaccgat
      301 ggcgcggccc ttgggcagtc ggccctggcc cgcgaccttg gcgatgtcga cgatttcctc
      361 gacgtcctgc acctcgatga tgccggcctg gcggaacgcg gcacgataga gatcgtagtt
      421 ccccgtcatg ttggccgtgt gcgaggccgc ggccttggtg ccgctctcgg tctgcgcgcc
      481 tttccagagg atgatcggtt tgccgagctc gagcgacttt cgaccaacct cgaggaagcg
      541 gcgcgcgtcc ggcgtgccct cgagataggc gaaggccagc ttcgtgccct catcgtcgag
      601 gaaggccgag agcagctccg gcatggtgat gtcggtctcg ttgcccgtcg agacgacatt
      661 gcggaagccg atgccctgcg cctcggcgag attgacgacg gcatagccga agccgccgga
      721 ttgaaaggcg cacgagaccg gacccgattt cagctcggtc tcgtggctga ccgagccgaa
      781 cacgcaccac atgcgcgacg gcaccgagag cgcaccctgg cagttcggcc cgatgacgcg
      841 gacgcccgtc tccttcacgg cgcgaatgag atcgtcctcg agcttgcnnn nnnnnnnnnn
      901 nnnnngtccc gcctcgcgga agcccgctgt caggatgatc gcgaacggga tcttggcggc
      961 accgcactcg cggaccgcgg caggcacacc tgcagccgga accgcaatca gtgcgagatc
     1021 gaccggcgag ccgatggcgg caacgctcgg gtagcacttc aggccatgca actcgccgta
     1081 cttcggattg acgagatgga ccggtccctt gaagcccgaa ttcttcagtg ccgcgatcgg
     1141 ctggccgctg atgcgcgcga gatcggcgga ggccccgacg acggcgatgg cattgggcga
     1201 gaacagtcgg gcaaacggcg acgtggccat ggcgtggcgt ttcctcgggt agcggcttgt
     1261 tgtcttgccc gaaagtcgtn nnnnnnnnnc cgctgtgtga agggcctcca ccgaggcatc
     1321 gccatgcacg gcgtgcttag ggccggcgga tcgcgacgtc cgaggagccg cccttgagac
     1381 gcggtcgcac cgtgtcgtaa agattgcact tcctctcggg gcgatagtac tcgatcatcc
     1441 ggcagcgggc gttggcgagg cagcgtgtgg cgcaggcctc gtaggcggca ccatatgtga
     1501 tgtcgaagcc cggtccttcg atccacatgc ccttgagccg ttccatcgtc gccgcggcgg
     1561 tcagtgtcgc cggtggcgcg gcaggcgacg gagactgcgc ggcggcgctc ccggcggcag
     1621 cggcaagcgc gagcccggcg aaagttgcgt tgagcaggcg tgagaagcgg ccgtgagcca
     1681 tgccagtctc cgtcaatgac cccatcgcga tatcgaacgg caatgagcac caaattttga
     1741 cccttctgga gcggtcgcgc ggcagcgcgt gcacgctgac gggcagatga cggatcgatg
     1801 cggctgtcgc ccgagtgttc gaggagaaga cgcgagcgag cgtgctcaat gtccgattgt
     1861 ccgccaaagc cgctggtcgt tcggccgtcg cgaccgtcgc gccgaccgac gtcggtgtcg
     1921 ggtgaggtcc gaccgctgtg ccaatggacg tcgggcgcgc ccttgtgccg atttcgtgac
     1981 gatcaagctc gggtagcagc acgctctctg gtgtggcgca ttgcgtcaga cgagcgggtc
     2041 gaacatgatc tctcgaacct cctgcgcgca tcggcacgcg gcagcaaatc tggcagccca
     2101 ctccagcgcg acctcgcgtg agggcacgtc gatgatcacg aacccaccga caaccgcctt
     2161 ggtctccgga tagggtccgt cagtgaccgt tccatctgcg gccactatgc tcgccctctg
     2221 gcttgccacc ccgccgccaa agatccacac ccctgcctcc tgagccgctc gaactacggc
     2281 atgcgccgcc tcgctgactg ccggcagctc ctgttcaggt ataaccatcg cgccatcgtc
     2341 aaacgagatt aggtaccgcg tcacctcgcg tctccgtagc cttgcgtcgt ccgcctgtgg
     2401 ctgtctcttc ccccggctct tgtaacctac caacggcggt aacggttgca acccgtccac
     2461 tcggcgacag ccccggggcg gcaacggatg tcttcgcgcc ggcgcggaca aaggccgcgt
     2521 gatgcgcctc cgcatctttc cagatcatgc cagagacccc gctgctgaaa cgacatgccg
     2581 gcgctgcatc ggcgatcgtc ggccatcaca gcgccgctcc catcgcagca gcgatgatct
     2641 gcatgtcggc gccggaaatc tcgaacaatc cgaagcggag ctgatagccc cagcactgcc
     2701 cgtcacgcgt gaaggctagt cggccgagca gcggtcgaat cggcgccgcg tccgcctcgg
     2761 cccattgcac gttgcgacgg aaaggacaga acccgccgcc catgtcggct tgatagggag
     2821 cgcccgaggc gacgatcccg atcgcggtga atgcctgcag cttctccttg ccccggaaat
     2881 gctcggtcgg ggaatagtac gcgatgctgt ggcccagcga gaggcgacgc agcggtgccg
     2941 ccttcccgtg acagacctgc atgaagccct gtgcccggcc gattttcaca tgttccgccg
     3001 aggctacggc gatccagctg cgctggctca tgcggcctcc ggcacgaaga cgcgcaggcg
     3061 atgcccatcg ggatcgagcg cgacgaaagt ggtgccgaag tccatgcgcg tcggtgtctg
     3121 cgcaatagca agtccgcgct tcttccaggc cgtgaatgtt tcgctgacag cggccgcgtc
     3181 cgcgaccgtg aacgccacct cgctgctgcc ggggtgtcct gtggtcttcg gctcgaccga
     3241 ggcgcgcgac cacagtccga gcatcacgcc gtcacgcagc ggcagcatgg caaaggtcgg
     3301 cgattgctcg acgatgggga tgtcaaacag ctcattgtag aacttggcgc tgacggccgg
     3361 gctttcgacg tggagcagaa gaatgctgaa gtctggcatg ggtggtctcc gtgttcggcg
     3421 cccgctgaag cgggtgcggt ggagaacctc tagctcggta tgctgtcaat ttatgtcagt
     3481 agcgtcaaag cttgtcattg ctccggcacg cctgccagtt cgcgccactc tttgagcaag
     3541 gccgcgcgtc gtctcggata gcgcttggcc gttggcttca gcgccgtgat gcggtccgtc
     3601 ctgaaatggc ggtagccgtc gcgaagctcg caccaggcga cgacgacgcg cgcgcgatcg
     3661 aagaatgcga gagcgaacgg ccagatcgtg cgctttgtcg ggctcccctt gccgtcggca
     3721 taggcgatct gcagcttgcg ctcggcacgg atcgcttcgc ggatcgaggc gagctccgca
     3781 tcgccagctg cgataggctc gctcgggccg atcagcaggc tcgacgcatc gaggcttgcc
     3841 cgcagatcgt cgggcagaac cgcgccgatc ttggcaagcg cgttgcgagc ggctgagccg
     3901 agcggaccgt ccgtgcgctc cgacacccaa cgcgagccga gcaccagcgc ctcgatctcc
     3961 tcctcgctga acatcagcgg cggcagcatg aaacccggac gcaccacgta gccgacaccg
     4021 gcttcgccat cgatgtgcgc accctgggat gtcgcgatag agcgttcgca gcgaaacccc
     4081 gacctcgtcc gcgagccttg cgcccgctac cggacggcga tgccgacgca agatctgaat
     4141 caagtcgaga aggcgctgcg agcgagacat cgccgtgcct taacatgaag ccaaggctta
     4201 atgctgccaa aaaatggcaa ctgcactact tgacccggaa ctcacgccgc gaccagcgcc
     4261 ccatccgagc gccgtgcacg cgcgcgcagc atcagccagg cgacaatcgc gaagttcagg
     4321 atgttccagg cgacgccatg tacgaaagcg gccttgtagg atcccgtata gtcgaagatc
     4381 gcgccggaaa cccagccgcc gagcgccatg ccggcgatcg tgctgccgag cacgagcccg
     4441 aacttggcgc ctgcctccga cggcgggaag tgctcgcgga tgacgatcgc atagctcggc
     4501 acgatgcccc cctggacgat gccgaagaac gccgagatca gatagagcgc gggcagactg
     4561 tcgaacggaa tgtagagggc gagcgcgatc gcctgcaagg ccgagccgac gatcagcgtc
     4621 gggaatccgc caaccttgtc gaggacccag cccgagacga ggcggctgat cacgccgaag
     4681 ccaagcagca cggcgagcat gtcggcgcca tgctgcgcgc catacttgag atcgccgcag
     4741 taggcgacga tgtgcgcctg cggcatcgac atggcgacgc agcagctgac gcccgcgacc
     4801 atcaggaggc cgatcatgat gccgggcgac atcggcatgg gcttcaggcc cttgccggcg
     4861 ccacccgcgg cagcggctgc gggcttcggc gcgcgccggg atgacgttgt tcgccgggtt
     4921 gccgacgtcg ac
//
LOCUS       DAORNH010000327         4930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12735_length_4930_cov_2.635897, whole genome shotgun sequence.
ACCESSION   DAORNH010000327 DAORNH010000000
VERSION     DAORNH010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12735_length_4930_cov_2.635897"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>219
                     /locus_tag="PK264_22390"
     CDS             <1..>219
                     /locus_tag="PK264_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78652.1"
                     /translation="PKADAPKADAPKADAPKAADPKAVAAPSAAPAPKADAPKTVEAP
                     KAPTPTPAPAATPAPTAAPAVPVKPVDAG"
     assembly_gap    220..285
                     /estimated_length=66
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <286..790
                     /locus_tag="PK264_22395"
     CDS             <286..790
                     /locus_tag="PK264_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HRD78653.1"
                     /translation="DLFWVRMQERRGQMVATAFAHNGAFVINALENLAGGEAFIGLRG
                     RGVTERPFDRVEEIRKASEARFKAEEKQLEERFTVLKKSLAGVTLKGDGQLEIGDKEK
                     AAIETFKSELVSVRKKLRDVQLAQRTDIDSLASRLKFWNIYAIPLLIGVAGMIAMAMR
                     RRRQAGT"
     gene            801..1823
                     /locus_tag="PK264_22400"
     CDS             801..1823
                     /locus_tag="PK264_22400"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRD78654.1"
                     /translation="MIKPQVFAGLVAAAVASLAVAVPLHTFYDRWTLPKATGELLVPD
                     LASKFKDIQAVEIVHQGKTLTLTRSGNIWGAKERSGYPVGIDRIAELLKKVALAELVE
                     AKTKSKDRLAILELEDPAGPKANSKRVRIKSAKDEVLADVVIGKSKLGAFGGAPGTYV
                     RRINETQAWLASGDPRPSADLKDWVPTAFLEQDTSKIAKVSIEIKGEPPLKLERAATP
                     GAKLTMVDAPAGKKMKEGQTIDAIGAALASLDLEDVRAVNPGPIAPGTHIVRFEEKDG
                     IAVTLHFRPEGDAHWVAVEATGTTDAAKKTAEAIMTRTRGWEYKLGKYKTDQILKRRT
                     DLFEGS"
     gene            complement(1827..4490)
                     /gene="pepN"
                     /locus_tag="PK264_22405"
     CDS             complement(1827..4490)
                     /gene="pepN"
                     /locus_tag="PK264_22405"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HRD78655.1"
                     /translation="MPQTEDGKPKAIRLDDYAPPAFLIDQVSLDVSLHPTRTKVRARS
                     KFRRNPASAAKAAGLRLDGEFLELEQVRLDGRVLATADYTLTSDALIIAKVPDDPFSL
                     DITTYCNPEANKALQGLYRSRGIYCTQCEAEGFRRITYFLDRPDVLSRYSTRIEADPR
                     EVPVLLGNGNLAETGVLDRGKRHYAVWKDPHPKPAYLFALVGGDLRSITSSFKTRSGR
                     KVDLGIYVEPGKEDRCGWAMESLKRSMRWDEERFGREYDLDVFNIVAVSDFNMGAMEN
                     KGLNIFNDRLVLATPETATDATYERIEAVIAHEYFHNWTGNRITCRDWFQLCLKEGLT
                     VYRDQEFTADERDRTVERITSVIDLKARQFPEDAGPLAHPVRPDTYIEINNFYTPTVY
                     EKGAELVRMIETIVGPDGFRRGMDLYFERHDGEAATVEQFVACFEAAAGADLRQFMTW
                     YAQAGTPELVCKFRYDPRRKIAELAVEQVIAPTPGEPRKKPLHIPVRIGLVGPNGGDM
                     PLRLESGAEVADGILHLRKRSEVFRFVDVASRPVPSLLRGFSAPVNLSLDLDDQDLVF
                     LMAHDSDRFNRWQAANSYAMRLMVAAAAALRAGRRPSRPTSFISALSEIVRDERLDPS
                     YRALMLQLPAETDVARVNGRNVDPALIHRARRAVIRAIAAALAEPLEAIYQAGGREKG
                     FKPDADSAGRRSLRNAVLALLTERGRAADIARAESHFRKADNLTDEIAGLTALAGQRG
                     DVRDAAFARFHQRWKGDHLVIDHWFSLQAASPLPTALRDVERLGRDPLFSLQNPNKVR
                     ALYVTFAQSNPVGFNRPDGRGYELIVDKVLAIDGFNPQIAARLLGSFRSWRALEPERR
                     KLARRAIARVLKADELSRDTYEIASRMLEER"
     gene            complement(4628..>4930)
                     /locus_tag="PK264_22410"
     CDS             complement(4628..>4930)
                     /locus_tag="PK264_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78656.1"
                     /translation="IARVVLKNAPILVLDEATSALDSEAEAAIQEQLFNLMTGKTVIA
                     IAHRLSTIAAMDRLVIMDEGRIVEEGSHDELLRRGGLYAGLWARQSGGFIVREAAQ"
BASE COUNT          843 a         1589 c         1653 g          779 t
ORIGIN      
        1 acccaaggcc gacgcaccca aggccgacgc accgaaggct gatgcgccga aggctgccga
       61 ccccaaggct gttgcggcac cgtctgcggc ccccgcgccg aaagccgatg ccccgaagac
      121 cgtcgaggcg cccaaggcgc cgacgccaac tccggctccg gcggcgacac cggcgcccac
      181 ggcggctccc gccgtacccg tgaagcccgt ggacgcgggn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggacc tcttctgggt
      301 ccgcatgcag gagcggcgcg ggcagatggt ggccaccgcg ttcgcccaca acggcgcctt
      361 cgtgatcaac gcgctcgaga acctcgccgg cggcgaggcc ttcatcgggc tgcggggtcg
      421 cggcgtgacc gagcgtccct tcgaccgggt cgaggaaatc cgcaaggcga gcgaggcgcg
      481 attcaaggcg gaggagaagc agctcgagga gcgcttcacc gtcctgaaga agagccttgc
      541 cggcgtgacg ctgaagggcg acggccagct cgagatcggc gacaaggaaa aggcagcgat
      601 cgagaccttc aagagcgagc tcgtttccgt caggaagaag ttgcgcgacg tgcaactcgc
      661 ccagcggacg gacatcgaca gcctcgcctc gcgcctcaag ttctggaaca tctatgccat
      721 tccgctgctg atcggcgtcg cgggaatgat cgccatggcc atgcgccgcc ggcgccaggc
      781 cggcacgtga gggacgcgtc atgatcaagc cgcaggtttt cgcaggtctc gtggcggcgg
      841 ccgtcgcctc gctcgcggtg gccgttccgc tccacacctt ctacgaccgc tggacgctgc
      901 ccaaggcgac cggcgagctg ctcgtcccag acctcgcctc gaagttcaag gacatccagg
      961 cggtcgagat cgtccaccag gggaagacgc tgacgctcac gcgttcgggt aacatctggg
     1021 gcgccaagga gcgctcggga tacccggtcg gcatcgaccg catcgccgaa ctcctcaaga
     1081 aggtcgcgct cgccgagctc gttgaggcga agaccaagag caaggaccgt ctggccatcc
     1141 tcgagctcga ggatccggcc ggtcccaagg cgaattcgaa gcgcgttcgc atcaagtctg
     1201 ccaaggacga ggtgttggcc gacgtcgtga tcggcaagtc gaagctcggc gctttcggcg
     1261 gcgcgcccgg cacctatgtg cgccgcatca acgagacgca agcttggctc gcgagcggcg
     1321 acccgcgccc gtccgccgac ctcaaggact gggtgccgac ggcattcctc gagcaggata
     1381 cgagcaagat cgccaaggtc tcgatcgaga taaagggcga gccgcccttg aaactcgagc
     1441 gcgcggcgac gcccggcgcc aagctcacca tggttgatgc ccccgccgga aagaagatga
     1501 aggaaggcca gacgatcgac gcgatcggcg cggcacttgc ctcgcttgat ctcgaggatg
     1561 tccgggcggt gaacccgggc ccgatcgcgc ccggcacgca catcgtccgc ttcgaggaaa
     1621 aggacggcat cgccgtcacg ctgcatttcc gaccagaggg cgacgcgcac tgggtcgcag
     1681 tcgaggcgac cggcacgacg gacgccgcca agaagacggc tgaagccatc atgacgcgca
     1741 cgcgcggctg ggaatacaaa ctcggcaagt acaagaccga ccagatcctg aaacgccgga
     1801 cggacctttt cgaggggagc tgatcctcat ctctcctcga gcatgcgcga ggcgatctca
     1861 taggtatcac gcgaaagctc gtctgctttc agaacgcgcg caatggcccg ccgcgcgagc
     1921 ttgcgccgct ccggctcgag cgcccgccag ctccggaacg agccgagcag ccgcgcggcg
     1981 atctgcgggt tgaagccgtc gatcgccagc accttgtcga cgataagctc atagccgcgc
     2041 ccatccgggc ggttgaaacc cacgggattg ctctgcgcga aggtgacgta gagcgcgcgc
     2101 accttgttgg ggttttgcag tgagaagagc gggtcgcgtc cgaggcgctc gacgtcgcgc
     2161 agcgccgtcg gcaaggggga ggccgcctgc agcgagaacc agtggtcgat gacgagatga
     2221 tcgcccttcc agcgctgatg gaagcgggca aacgcggcat ctcggacgtc accgcgctgg
     2281 ccggcaagcg ccgtgagacc tgcgatttca tccgttagat tgtcggcctt gcggaaatgg
     2341 ctctcggccc gggcgatgtc tgccgcccgc ccgcgctcgg tcagcagtgc gagcacggcg
     2401 ttcctgaggc tgcgcctgcc cgcactgtca gcatccggct tgaaaccctt ctcgcgcccg
     2461 ccggcctggt agatggcctc gagcggctcg gcgagggccg ccgcgattgc ccggatgacc
     2521 gctcgccgcg cgcgatgaat gagggccgga tcgacattgc ggccgttgac ccgggcgacg
     2581 tcggtctccg ccggcagctg cagcatgaga gcgcggtagg aaggatcgag gcgttcgtcc
     2641 cggacgattt ccgacaaggc tgagatgaag ctcgtcggac gcgacgggcg ccggccggcc
     2701 cgcagggctg ctgcggcggc aaccatgagg cgcatggcgt agctgttggc tgcctgccag
     2761 cgattgaagc ggtcgctgtc gtgggccatc aggaacacga ggtcctggtc gtcgagatcg
     2821 agagagagat tgaccggcgc cgagaagccg cgcagcagcg agggcaccgg ccggctcgca
     2881 acgtcgacga accggaacac ctcgcttcgt ttcctcaagt gcaggatgcc gtctgcgacc
     2941 tccgcgccgc tctcgagccg caggggcatg tcgccgccgt tcgggccaac gagcccgatc
     3001 cgaacaggaa tgtgcagcgg cttcttcctc ggctcacccg gcgtcggcgc gatcacctgc
     3061 tcgacggcga gctcggcgat cttcctgcgc ggatcgtagc ggaacttgca gacgagttcc
     3121 ggcgtgccgg cctgcgcgta ccaggtcatg aactggcgga gatcggcgcc ggccgccgcc
     3181 tcgaagcagg cgacgaactg ctcgacggtc gccgcctcgc cgtcgtgacg ctcgaaatag
     3241 aggtccatgc cgcgtcggaa gccatccggg ccgacgatcg tctcaatcat gcggacgagc
     3301 tctgcgccct tctcgtagac ggtcggggta tagaagttgt tgatctcgat gtaggtgtcc
     3361 ggtcgcacgg ggtgggcgag aggccccgcg tcctccggga actggcgcgc cttcaggtcg
     3421 atcacgctcg tgatgcgctc gacggtccgg tcgcgctcgt cggccgtaaa ctcctggtcg
     3481 cgatagaccg tgagcccctc cttgaggcag agctggaacc agtcgcgaca cgtgatgcga
     3541 ttaccggtcc agttgtggaa gtactcgtgc gcgatgacgg cctcgatgcg ctcgtaggtg
     3601 gcgtccgtcg cggtctcggg cgtcgcgagc acgaggcgat cattgaagat gttgagcccc
     3661 ttgttctcca tggcgcccat gttgaagtcg gagacggcga cgatgttgaa cacgtcgaga
     3721 tcgtactcgc gcccgaaccg ctcctcgtcc caccgcatcg agcgcttcag gctctccatg
     3781 gcccagccgc agcgatcctc cttgccgggc tcgacgtata tgccgaggtc gaccttgcgc
     3841 cccgagcgcg tcttgaagct cgaggtgatc gagcgcagat cgccgccgac gagcgcgaag
     3901 aggtaggcgg gcttcgggtg cgggtccttc cagacggcat aatggcgctt gccgcggtcg
     3961 aggacgcccg tctcggcgag gttgccgttg ccgagcagga ccgggacttc gcgcgggtcg
     4021 gcctcgatgc gagtggagta tctggagagc acatccggcc ggtcgaggaa gtaggtgatg
     4081 cgccggaagc cctcggcctc gcactgcgtg cagtagatgc cgcgcgagcg atagagcccc
     4141 tgcagcgcct tgttcgcctc cggattgcag tatgtcgtga tgtcgagcga gaacgggtca
     4201 tcaggcacct tggcgatgat gagcgcatcg ctcgtcagcg tgtagtcggc ggtggcgagc
     4261 acccgaccgt cgagacgaac ctgttcgagc tcgaggaact cgccatcgag cctgagtccg
     4321 gccgccttgg cggcgcttgc cgggttccgc ctgaattttg agcgggcacg caccttcgtg
     4381 cgcgtcggat gcaacgacac gtcgagcgac acctgatcga tgaggaacgc cggcggagcg
     4441 tagtcatcga gacgaatggc cttcggcttg ccgtcttcgg tctggggcat tcagcttcat
     4501 ccagttcggc cttgaccggc tgcgaatcac cgccggctca ggcgtcatat ggcgtggcga
     4561 tcaatcgcga gggaagcgat ttcactcagc tggagagctc tcgagagccc tccgaccgag
     4621 gcctcggtca ctgtgccgcc tcgcggacga tgaagccgcc ggactggcgt gcccaaaggc
     4681 ccgcgtagag tccgcccctg cgcagcagct catcgtggct gccttcctcg acgatgcgcc
     4741 cctcgtccat gatgaccagg cggtccatgg cggcgatcgt cgagaggcgg tgggcgatgg
     4801 cgatcacggt cttgccggtc atgaggttga aaagctgctc ctggatcgct gcctccgcct
     4861 cgctgtcgag tgcgctcgtc gcctcgtcga gcacgaggat cggcgcgttc ttgagcacga
     4921 cccgcgcgat
//
LOCUS       DAORNH010000328         4894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12858_length_4894_cov_2.666460, whole genome shotgun sequence.
ACCESSION   DAORNH010000328 DAORNH010000000
VERSION     DAORNH010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12858_length_4894_cov_2.666460"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..467
                     /locus_tag="PK264_22415"
     CDS             117..467
                     /locus_tag="PK264_22415"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRD78657.1"
                     /translation="MDSIEASIELADHLGDVAGALALSLSTAPLGAQSALPADGAELA
                     RKLCSNCHGTDPDGGAPGRPDVPSFRVIANRSQTTPERLASAIILPHPEMPGIGLTRA
                     ETRAIIDYIMSLKK"
     gene            complement(478..1044)
                     /locus_tag="PK264_22420"
     CDS             complement(478..1044)
                     /locus_tag="PK264_22420"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HRD78658.1"
                     /translation="MISDLTSRVRRGLLLAVAAPVLATTAMPAAAQGLVSEIKVGALY
                     HDPGDLWSGFRREPQSVDLNLELLLAPHWNVLGGTLRPAIGGSINFEGGTSKAYLDAR
                     WEIEAPSGIFFSIGLGAAIHDGNIDPIAPDKKALGSRVLFHIPAEIGWRWDGRNSISV
                     FFDHVSNGYTQDYNEGLDSFGVRYGYRF"
     gene            1254..2621
                     /locus_tag="PK264_22425"
     CDS             1254..2621
                     /locus_tag="PK264_22425"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD78659.1"
                     /translation="MPKSIGIIGAGAGGLTGAKHMLEAGADVTIYEIGSHVGGLWGYQ
                     NDNKRSSAYKTLHINTARDLTAFADHPFPKTVQPFPDHRDMAAYLKSYAEHFGLMRLI
                     RFNTPVRDVRPAPGYRAEAPRWRLTLGNGETVEHDAVIVASGHLTKRLDVADFKEKFT
                     GEYLHSHDYKDPARFAGKRICIVGVGNSALDIASDVCSAAESCVLVARSRPLIIPKLI
                     FGRPFWDVIRPFYHPWVPAAVRNRVLKALVWTVHGDMGRLGFPAQSQKIHATSNANIV
                     NHIQYRRVLVKQGIERIAGRVVHFVDGTSEEFDTFIAATGYVLDLDFIPKEVVPIRNN
                     GLDLYMRIVPPDWRGLYFLGFFNSDTAANWIMEGQIRWLREFELGRAALPGKDVMMAS
                     IEARRYRVLRDFKDTPRHEIEVEHLPYFRELKTTLAEGLQRVGAKPRDVGIGAKGQVP
                     KFAPA"
     gene            complement(2744..4444)
                     /locus_tag="PK264_22430"
     CDS             complement(2744..4444)
                     /locus_tag="PK264_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HRD78660.1"
                     /translation="MKYMLAAGALLMSTAAATAADVAATSRIDAVTVYPQGAEVTRLA
                     KVKLVAGEHVVLLTDLPATAVASSIRVEGRATGKLDIASVDTRRTSIPSTDPARAEGE
                     RRRLEKEIEALNDKRIEVEARIQTAETQKALIAKLVELPVHPAPAGPGGTAAATDWGQ
                     VFTLIGARMAEAQKVIQAGQLEIREIARKIEDLQKQLVALAPAPLERTEVKVNVTAGT
                     ALEADLVIRYQVANAAWTPFYDARLSTGAKGAAAKLALVRRASIVQRTGEDWGDVTVS
                     LSTARPGTGTAAPVLNPMVVDFQPETPPAPPPPRPVAAPSPSPYAGRSMDDAMMRSAA
                     PAGTSAPTMKVALEESRAEILVTSFQAVFVVPGRQTVKGTGEQKRVLIDTTDLEPQLV
                     VRTTPRVDAKAYLYAKMTLPKGAPYLPGTVSLFRDQTFVGSGRLPQLAGGEEHELGFG
                     ADDAVRVRHALVEEKRGESGLISTSKNDERQFRIVLKSQHERPIQVIVLEQIPVSLNQ
                     EIKIEYLGRLQPTKQNVEDKRGIITFEFQLAAGEERTIDFGYRIVWPAAKRIIYNSGK
                     "
     gene            4734..4810
                     /locus_tag="PK264_22435"
     tRNA            4734..4810
                     /locus_tag="PK264_22435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4768..4770,aa:Arg,seq:acg)
BASE COUNT          810 a         1571 c         1661 g          852 t
ORIGIN      
        1 aggtcctgaa cgtcgggtcc tccttcgaga agcggttcaa cgccttgccg aggttggtcg
       61 agcccttggc gtccttgggc ttgatggcca tcttgatgac cggctcgggc acgaacatgg
      121 actccatcga ggcctcgatc gagctcgccg atcatctcgg cgatgtcgcg ggcgcgctcg
      181 cgctctcgct ttcaaccgcg ccactcggag cgcagagcgc tctcccggcg gacggggcgg
      241 agcttgcccg caagctctgc tccaactgtc acggcaccga tcccgacggc ggtgctccgg
      301 gccgtcccga cgttccgagc ttccgcgtca tcgccaacag atcgcagacg acgccggagc
      361 ggctcgcctc ggcgatcatc ctgccgcatc ccgaaatgcc cgggatcggg ctcacgcgtg
      421 cggagacgcg agcgatcatc gactacatca tgtcgctgaa gaagtgagcg gctgagccta
      481 gaatcggtag ccgtagcgaa ccccgaagct gtcgaggccc tcgttgtagt cctgtgtgta
      541 gccgttcgag acgtggtcga agaataccga gatcgagttg cggccgtccc aacgccagcc
      601 gatctcggcc gggatgtgga agaggacgcg ggagccgagc gccttcttgt ccggggcgat
      661 gggatcgatg ttgccgtcgt ggatcgcagc tccaaggccg atcgagaaga agatgccgga
      721 cggcgcctcg atctcccagc gtgcgtcgag gtaggccttg ctggtgccgc cctcgaaatt
      781 gatcgagccg ccgatcgcgg ggcgcagcgt gccaccgagg acattccagt gcggcgccag
      841 cagtaactcg agattgaggt ccaccgattg cggctcgcgc cggaagcccg accagaggtc
      901 gcccggatcg tgatagagcg cgccgacctt gatctcggac acaagccctt gcgctgccgc
      961 gggcatggcg gtcgtcgcga gaacaggcgc cgccacggct aagagcaggc cgcgccggac
     1021 gcgcgatgtg aggtcagaga tcataggatg gcttctaccg cgatttgccg gcgggtggag
     1081 tgcggattcg ggcgtttggg cgcgacagtt gggaccgccg ttgccaaagg gtcacgaaga
     1141 cgcgatccgg cacgtgcgtg gctgccctgc ctctcgcggg gaggtcggca cttgtgcggc
     1201 gcgccatccg cggcaaaaat gatcgcacga acacgtattg gaccgggatc gccatgccga
     1261 aatcgattgg aatcatcggg gccggagccg gcgggctcac gggcgccaag cacatgctcg
     1321 aggcgggcgc tgatgtcacg atctacgaga tcggcagcca tgtaggcggc ctctggggct
     1381 accagaacga caacaagcgc tcctccgcct acaagacgct gcacatcaac acggcgcgcg
     1441 acctcactgc cttcgccgac cacccgttcc ccaagacagt gcagccgttc cccgatcatc
     1501 gcgacatggc ggcgtatctc aagagctatg cagagcattt cgggttgatg cggttgatcc
     1561 gcttcaatac acccgtccgc gacgtccggc ccgcgccggg ctatcgggca gaggcgccgc
     1621 gctggcggct caccctcggc aatggagaga cggtcgagca tgatgccgtg atcgtcgcct
     1681 cgggccacct cacgaagcgg ctcgatgtcg ccgacttcaa ggagaagttc acgggcgaat
     1741 acctgcactc gcacgactac aaggatccgg cgcgcttcgc agggaagcgc atctgcatcg
     1801 tcggcgtcgg caacagcgcg ctcgacatcg cctccgacgt ctgctcggcg gcggagagct
     1861 gtgtgctcgt cgcccgctcg cggccattga tcattccgaa gctgatcttc ggccggccgt
     1921 tctgggacgt gatccggccg ttctaccacc cgtgggtgcc ggcggcggtg cgcaatcgcg
     1981 tgcttaaagc gctggtttgg acggtgcacg gcgacatggg tcgcctcggc tttccggcgc
     2041 aatcgcagaa gatccacgcc acctccaacg cgaacattgt caaccacatc cagtaccggc
     2101 gcgtgctcgt gaagcagggc atcgagcgga tcgcgggccg ggtcgtgcac ttcgtcgacg
     2161 gcacgtcgga ggaattcgac acgtttatcg ccgcgaccgg ctacgtgctc gatctcgact
     2221 tcatcccgaa ggaggtcgta ccgatcagga acaacggcct cgacctctat atgcggatcg
     2281 tcccgcccga ctggcgcggg ctctatttcc tcggcttctt caattcggat acggctgcca
     2341 actggatcat ggaaggccaa atccgttggc tccgcgagtt cgagcttgga cgggccgcgc
     2401 tgccggggaa ggacgtcatg atggcctcca tcgaggcacg ccgctatcgc gtgctccgcg
     2461 acttcaagga cacgccacgg cacgagatcg aggtcgagca ccttccgtac ttccgcgagc
     2521 taaagacgac actcgccgaa gggctgcagc gggtcggcgc caaaccccgc gacgtcggca
     2581 tcggcgccaa aggccaggtg ccgaagttcg cgccggcgtg agctgcattg gcgcacctcg
     2641 aacggcgccc ctcgaacgac aatgcccggc agggattgcc gggcattgta tgactggtgc
     2701 aagtcgagca gtgctgctac cgcgccgatg caccctgggc agattacttt ccggagttat
     2761 agatgatgcg cttcgctgcc ggccagacga tgcggtagcc gaagtcgatc gtgcgctcct
     2821 cgcccgccgc gagttggaac tcgaaggtga tgattccgcg cttgtcctcg acgttctgct
     2881 tggtcggctg caggcggccg agatactcga tcttgatctc ctggttgagc gagaccggga
     2941 tctgctcgag cacgatcacc tggatcggcc gctcgtgctg gctcttgagg acgatgcgga
     3001 actggcgctc gtcgttcttc gaggtcgaga tgagcccact ctcgccgcgc ttctcctcga
     3061 cgagggcatg gcggacgcgg acagcatcgt cggcgccgaa gccgagctcg tgctcctcgc
     3121 cgccggcaag ctgcggcagg cggccgcttc cgacgaaggt ctggtcgcgg aacagcgaca
     3181 ccgtgcccgg caggtagggc gcgcccttcg gcagcgtcat cttggcgtag agataggcct
     3241 tggcatcgac gcgcggcgtc gtgcgcacga cgagttgcgg ctcgaggtcg gtcgtgtcga
     3301 tgaggacacg cttctgctcg ccggtgccct tcacggtctg gcggcccggc acgacgaaga
     3361 cagcctggaa cgaggtcacg aggatctcag cgcggctctc ctcgagcgca accttcattg
     3421 tcggcgcaga tgtaccggcc ggcgcagccg atcgcatcat tgcgtcgtcc attgagcgac
     3481 cggcgtaggg cgacggcgag ggtgccgcga cgggacgcgg cggcggcgga gccggcggag
     3541 tctccggctg gaagtcgacg accatcggat tgaggacagg cgccgcggtt ccggtgcccg
     3601 gtcgcgcggt cgagaggctc actgtcacgt cgccccagtc ctcgcctgtc ctctgcacga
     3661 tggaggcgcg gcggaccagc gcgagcttgg cggcggcgcc ctttgctcct gtcgagaggc
     3721 gggcgtcata gaacggcgtc caggcggcgt tggcgacctg gtagcggatc acgagatcgg
     3781 cctcgagggc cgtgccggcg gtgacgttga ctttgacctc tgtgcgctcg agcggtgccg
     3841 gtgccaaggc gacgagctgc ttctgcaggt cctcgatctt gcgggcgatc tcgcggatct
     3901 cgagctggcc cgcctggatc accttctgcg cttcggccat gcgcgcgccg atcagcgtga
     3961 agacctgccc ccagtcggtc gccgcagcag tccctccagg acccgccggc gcgggatgga
     4021 cagggagttc gacgagcttg gcgatcagtg ccttctgcgt ctcagcggtc tggatgcggg
     4081 cctcgacctc gatgcgcttg tcgttgagtg cctcgatctc cttctcgagc cggcgccgct
     4141 cgccctccgc gcgcgcgggg tcggtgctcg gaatcgaggt gcgccgcgta tcgaccgagg
     4201 cgatgtcgag cttgcccgtc gcgcgccctt cgacgcggat cgagcttgca accgcggtcg
     4261 ccgggaggtc ggtcagcagg acgacatgct cgccggccac gagcttcact ttggcgagcc
     4321 gcgtcacctc ggcgccctgc ggatagacgg tgacggcatc gatgcgcgag gtcgcggcga
     4381 cgtccgcggc ggtggcggcg gcagtgctca tgagcagggc tcctgcggct agcatgtatt
     4441 tcatgggcgg ctccaacgga aagtgcgacg agcagttaca acttggtgtg tgatcgaggc
     4501 tgccagcata gacggcccgg atcgtggcga ccttggggtc cggggtacac ggccggcagg
     4561 gcagatcgga tgtcggaccc gcagggctcc gggcacgtca ggtccacggc gctcacggac
     4621 gagcctcgcg caggaaaatg cctggcctcg cacgccgccg ccggaggcca cgcagaacag
     4681 catgcggaag cgcttgcgtt ttcggaactg ccagcctaga gagtgcagcg cacgcgccgg
     4741 tagctcagtt ggatagagcg ccagactacg gatctggagg tcgtgggttc gaatccttcc
     4801 cggcgcgcca cttcggtacg gtccgttccg gacacatcgg ttacggttta ttcccgagac
     4861 atgggtaata cattgcggac ctcgaagggg tttg
//
LOCUS       DAORNH010000329         4865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_12965_length_4865_cov_2.344283, whole genome shotgun sequence.
ACCESSION   DAORNH010000329 DAORNH010000000
VERSION     DAORNH010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12965_length_4865_cov_2.344283"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK264_22440"
     CDS             complement(<1..260)
                     /locus_tag="PK264_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRD78661.1"
                     /translation="MPIVPATPDAISAAARLLAEGRLVAFPTETVYGLGADATNGAAV
                     AAIFAAKGRPRFNPLIVHAADAERAFRIGRPTPIAAALAAAF"
     gene            781..1632
                     /locus_tag="PK264_22445"
     CDS             781..1632
                     /locus_tag="PK264_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78662.1"
                     /translation="MIVEADAEAWPLFLPSQQGAQSATDHRAEYGEVARVGVLEVADL
                     TAQHRIQVGDDALEAIAPRAVGLGPDAVFEFGQVLLANVAVASAKPVAEGPWPGPEPG
                     STPCPGTRQSPICLIAGCSVRPFPLSHSRTIASAACASSRERHRIRKSSAERTSGPCW
                     LATTHHRNGFDILRAARSPRAALHPASPHQTLLWLMSGDRRFGSCSQLCHRCVEVDVE
                     RIGEDAAQEPRRKSRSAICQFVASAELPSHDELWRFSIEAMSANAIVARWSFGIAELR
                     RTDASTS"
     gene            2050..3237
                     /locus_tag="PK264_22450"
     CDS             2050..3237
                     /locus_tag="PK264_22450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78663.1"
                     /translation="MPAPAAADLTMPIAQQWTDIRDGVAKVCEAFPNDYWVKLDHAAE
                     YPSEFVKALTEAGYLSALIPEAYGGSGLPLSAGCAILETIHSTGCNAAACHAQMYTMG
                     TVLRHGSEEQKKKYLPGIASGELRLQAFGVTEPTTGSDTTQLKTKAEKRGNKYVVNGQ
                     KVWTSRVLHSDLMLLLARTTPADKVKRRSDGLSVFLVDLREAKGRGIEIKKIDAMINH
                     NTCEVFFDNLEVPPDALIGEEGQGFKYILSSMNAERILIASECLGDARFFIERAAAYA
                     KERVVFGNPIGKNQGVQFPIARAYAELIAAELVVRKAAAMYEAGLDCGEEANIGKMLA
                     ADAAWKAAEACLQTYGGFGYAREYGIERKWRECRLYQTAPISTNMVLAFVGQNVLGLP
                     RSY"
     gene            complement(3506..>4456)
                     /locus_tag="PK264_22455"
     CDS             complement(3506..>4456)
                     /locus_tag="PK264_22455"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HRD78664.1"
                     /translation="DILQVSSEVAGRIIELKVREHETVKAGDVMVRIDPDPYAMALTK
                     AEAELDAMRAQVATLIASWHEADSELKEADSRLTFLDVQLNRQKQLAKSGVVATVKLE
                     DAESNASQARERVAVMRQKLARVAAQIGGDPKQPVDMHPLVREKMALRDRAKLDLART
                     IITAPASGIVVNAKLLVGEHIKAATPLFALVADSQPWVEANFKETELTNVRPGQSATV
                     TLDIYPDIVWTATVDTISPATGAEFALLPPQNASGNWVKVVQRLPVRLKLVPVAGRPP
                     LRAGMTATVKVDTEQKRRLAQLIGWATAEAKTSPAPAASK"
     assembly_gap    4457..4507
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4581..>4865)
                     /locus_tag="PK264_22460"
     CDS             complement(4581..>4865)
                     /locus_tag="PK264_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78665.1"
                     /translation="GWVERRPDPDDRRINRMHLTDRGRRVHAAIWPIALSTVDDALAR
                     LSNDERDVLVELLGRVKGELVELAGADNGQSISAEEILGASDDDMSEARA"
BASE COUNT          833 a         1655 c         1557 g          769 t
ORIGIN      
        1 cagaaggccg ccgcaagcgc cgccgcgatc ggcgtcggtc ggccgatccg gaacgccctt
       61 tccgcatcgg ccgcgtgcac gatcagcgga ttgaagcgcg gccgcccctt ggccgcgaag
      121 atcgctgcga ctgccgcgcc gttcgtcgca tcggcgccga ggccgtagac cgtctcggtc
      181 ggaaaagcga cgagccggcc ctcggccagc aggcgcgctg cggccgaaat cgcgtcaggt
      241 gtggccggga cgatcggcat atggctccca tcataatatg cggcacgctt agcgcgcagc
      301 cggcaagccg acaacctcgg acggcagcat ccgtggccgt tccgcacgat ccgcagtcac
      361 cttcccgccc gcgccgtcat gccgacgccc cgtggacctc ggagccgcca ccgcgtatcc
      421 acagcacgat gacctcagtc ctcgcctacg cgaggatgac gaggtcgcgc gggaagacga
      481 gggtcgcgcg ggagtaccga tcctcggtgt cccggaactc ggacgccgcg cagcggccgg
      541 ccgaatgtca gggatcgttc cagctgctcg gatgcccagc cgcacctccg gcgaccacac
      601 ctcaattggc aatagggggc gggaccggct ttcgccgctc ctgcccactg aaccgtacgg
      661 gcggatttcc gtttacggct ctccagccgg tggtttcgac atcgagactg tctcgctagt
      721 tgtcaggctg tgtgaagcga acagaccggc attcgcgaag taggcattcg gccagcgcag
      781 gtgatcgtcg aggcagatgc cgaggcctgg ccgctgttcc tgccgtcgca gcaaggcgcg
      841 caatcggcga cggaccatcg agccgaatac ggcgaagtcg cgcgggtagg cgtgcttgag
      901 gtagccgatc taaccgcgca gcataggatt caggtcggcg atgacgcact cgaggctatc
      961 gctccgcgag cggtcggact tggcccggat gcggtctttg agtttggcca ggttcttctt
     1021 gcgaacgtag cggtggcgag cgccaaaccg gtagccgagg ggccctggcc cggccccgag
     1081 ccgggctcga ccccttgtcc cggcacccgg caatcgccga tatgcctcat cgcgggatgc
     1141 agcgtgaggc cattcccgct ctcccacagc cgcaccatcg cgagtgcggc ctgcgcctcc
     1201 tcgcgtgagc ggcacaggat caggaagtcg tcagcagagc gcaccagcgg gccttgctgg
     1261 ctcgccacca cgcaccaccg aaacgggttc gacatcctgc gggctgctcg ttcgcctcgg
     1321 gcggccctcc accccgcatc gccgcaccag acgctgctat ggctcatgag tggtgatcgg
     1381 cgttttggat cgtgcagcca actctgccat cgctgcgtcg aggtcgacgt tgaacggatc
     1441 ggtgaagacg ccgcccagga gccgaggcgg aaatcgcgaa gcgcgatctg ccaattcgtc
     1501 gcctcggccg agcttccgag ccacgacgaa ctgtggcgct tctcgatcga agcgatgtcc
     1561 gcaaacgcaa ttgttgcgcg atggagcttc ggcatcgcgg agctgcgccg gactgacgcg
     1621 tcgacgagct gaaagctcat cgcgttcgcg ctcatcgaca gccaaatcct gcggcggctc
     1681 cagctcgcaa tgtagatgcc cgggccgaag agcaccaagg ccatcaagag cgagggaatc
     1741 caggcgggtg cagcgggaga tcggggtggc cgggtcctgg tgccgaggac cgtcgtcacg
     1801 agacctgcaa aaagtaccgc accgagtatc aggagcgcgg caccgaaata ggcgccgacc
     1861 aacgagcgca gccttggacg atagacgaca ccctcgaccg gcatgcagcc aacctcatcc
     1921 gagcgcgatt ttcagcagtc gagtacccta ccacgcctgg acgctcgaca acaccaacac
     1981 cttgctgttc gggcctctcg gccctatccg agtgtggagt ttccctctcc cccgaccgag
     2041 gacccgccca tgcccgcacc cgccgcagcc gacctgacga tgccgatcgc ccagcagtgg
     2101 accgacatcc gcgacggcgt cgccaaggtc tgcgaggcat tcccgaacga ctactgggtg
     2161 aagctcgacc acgcggcaga gtatccgagc gagttcgtga aagccttgac ggaggccggc
     2221 tacctcagcg cgctgatccc cgaggcctat ggcggctccg ggcttcccct atccgccggc
     2281 tgcgcgatcc tcgagacgat ccattcaacg ggctgcaacg ccgccgcctg ccatgcgcag
     2341 atgtacacca tgggcaccgt gcttcggcac ggctcggagg agcagaagaa gaagtacctg
     2401 ccgggcatcg cctcggggga gctccgcctg caggccttcg gcgtcaccga gccgaccacc
     2461 ggctccgata cgacgcagct caagaccaag gccgagaagc gcggcaacaa gtacgtcgtc
     2521 aacggccaga aggtgtggac gagccgcgtc ctccactccg atctcatgct gctgctcgcc
     2581 cgcactacgc ccgccgacaa ggtgaagcgc cgctcggatg ggttgagcgt atttctcgtc
     2641 gatctgcgcg aggcgaaggg gcgcggcatc gagatcaaga agatcgacgc gatgatcaat
     2701 cacaacacat gcgaggtgtt cttcgacaat ctagaagtgc cgccggacgc gctgatcggc
     2761 gaggagggcc agggcttcaa gtacatcctc tcgagcatga acgcggagcg aatcctgatc
     2821 gcgtccgaat gcctcggcga cgcccgcttc ttcatcgaac gcgccgcggc ctacgccaag
     2881 gagcgcgtcg tgttcggcaa cccgatcgga aagaaccagg gggtgcagtt cccgatcgcg
     2941 cgcgcctatg cggagctcat cgccgccgag ctcgtcgtgc gcaaggccgc cgccatgtac
     3001 gaggccgggc tcgactgcgg cgaggaagcc aacatcggca agatgctcgc cgcggatgcg
     3061 gcctggaagg cggcggaggc gtgcctgcag acctacggcg gcttcggcta tgcgcgcgag
     3121 tacggcatcg agcgcaagtg gcgcgagtgc cggctctatc agacggcgcc gatctcgacc
     3181 aacatggtgc tcgcattcgt cggccagaac gtgctcggcc tgccgcggag ctattgagcc
     3241 gatgcgctgg ctccgtgcca cagcgatcgc attgggcgcg ctagtgatcc tggcgtcggc
     3301 cggcgtcgca tggatcgacg tcgagcgcac ccgcatccgc caggcctgcg acgcactcct
     3361 aacgagaggc atcgacgcga cgttctaccc gcacacctgt ttcctgaaag gtgcccgcca
     3421 gccgatgtgc tggcgcgcca cctgtatccg tgaccgcacg cacggcccta gtctggagcg
     3481 aacgtgaggc agccgagccg ccgccttact tgctcgccgc tggtgccggg ctcgtcttgg
     3541 cctcggctgt ggcccagccg atcaactggg ccagccggcg cttctgctca gtatcgacct
     3601 tgacggtcgc cgtcatgccg gcgcggagcg gcggacggcc cgcgaccggc accagcttga
     3661 ggcgcacggg cagtcgctgc acgaccttca cccagttgcc gctcgcgttc tgcggcggca
     3721 gcagcgcgaa ctcggccccc gtcgccggac tgatcgtatc gaccgtcgcc gtccagacga
     3781 tgtccgggta gatgtcgagt gtcaccgtcg ccgattggcc cggccggacg ttcgtcagct
     3841 cggtttcctt gaagttcgcc tcgacccagg gctggctgtc ggcgacgagc gcgaacaggg
     3901 gcgtcgccgc cttgatgtgc tcgccgacca gcagcttcgc attgacgacg atgccggagg
     3961 ccggcgccgt gatgatggtg cgcgcgagat cgagcttggc gcgatcgcgc agcgccatct
     4021 tctcgcgcac gagcggatgc atatcgaccg gttgcttcgg atcgccgccg atctgggccg
     4081 cgacgcgcgc gagcttctgg cgcatcacgg cgacgcgctc gcgggcctgg gacgcgttac
     4141 tctcggcatc ctcgagcttc accgtcgcga ccacgccgct cttggcaagc tgcttctgcc
     4201 ggttgagctg cacatcaagg aaggtcagcc ggctgtcggc ctccttcagc tcgctgtcgg
     4261 cctcgtgcca gctggcgatc aaggtcgcga cctgcgcgcg catggcatcg agctcggcct
     4321 ccgccttggt cagcgccatg gcatagggat cgggatcgat acgaaccatg acgtcgccgg
     4381 ccttgacggt ctcgtgctca cgcaccttga gctcgatgat gcggccggcg acctccgacg
     4441 acacctgcaa gatgtcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnncca gccgagcgcc gccgccacca cgaggcacgg caccacgacg atcaggacac
     4561 ctctgaccca actacgcatg ttaagctcgc gcctcgctca tgtcgtcatc gctcgcaccg
     4621 aggatctcct cggcggaaat ggattgtccg ttgtcggcgc cggcaagctc gacaagttcg
     4681 ccctttaccc gaccgagcag ctcgacgagc acatcgcgct cgtcattgga aagccgcgcc
     4741 agggcgtcat cgaccgtcga cagcgcgatc ggccagatgg cggcatgcac gcggcggccg
     4801 cgatcggtca ggtgcatgcg attgatgcgc cggtcgtcgg gatcgggacg ccgctcgacc
     4861 cagcc
//
LOCUS       DAORNH010000330         4761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13334_length_4761_cov_2.769868, whole genome shotgun sequence.
ACCESSION   DAORNH010000330 DAORNH010000000
VERSION     DAORNH010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13334_length_4761_cov_2.769868"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PK264_22465"
     CDS             <1..296
                     /locus_tag="PK264_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78666.1"
                     /translation="VVAMPVGDEQVAGWLAALDEMEAVLDGRKLLPHWRLSQGFDLKQ
                     VLEEPKPFDLVLWITGHAALPYTKPGETVSSATWANWQRVFGGNFLAYAFWFN"
     gene            complement(304..897)
                     /locus_tag="PK264_22470"
     CDS             complement(304..897)
                     /locus_tag="PK264_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRD78667.1"
                     /translation="MAGDAEVGHSPNRSAPGPIRILLDGIDALCRIDGWMAAVCLALL
                     TLLILAEITVRALSHYFPSVPADIPVAWEYSSYLMAATFTFGAAMTLRSGGHIRVTLL
                     LARLPVPLRRAAEVLVSAAGLMMAGFLTYAMFRFTYEAFSRGQTSISSDTRLWIPQAI
                     VTFGILLLALQFFARFIQALLGLPLEDQRLKVTTLSE"
     gene            complement(927..2102)
                     /locus_tag="PK264_22475"
     CDS             complement(927..2102)
                     /locus_tag="PK264_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRD78668.1"
                     /translation="MRFGSAAPGYATYGASADHTDRSFAKEWEAKPPKGFATLSARNL
                     GPMKAAIQLYADVVAKGGFPVIPEVPLQIGMNHPAVGVLRQRLMMSGDLKEAGFAISD
                     AFDGYVEKAVRRFQASNGLTPNGIVDKRTIAALNVPAAARLRQLRLNLTRLSEFSRPG
                     GRYIMVNIPAAQIEVVEGDIVVSRHAGVVGKIDRQTPILKSQIHELNFNPVWHLPPTV
                     IEKDLIPKGMEMSRRGQNVLAKYGIDAYGPDGRKIDPLRVNWSMARSLSFKQTPGPEN
                     PLGFVKINFHNSHSVYMHDTPSHTLFGRNFRAASSGCVRISGIEHVAAWLMQDQGWTP
                     ERVMEMKKSGERLDVKLKKPIPLYFAYITAWATEDGVVQFRRDLYNKDGVGPTASAY"
     gene            complement(2503..3465)
                     /locus_tag="PK264_22480"
     CDS             complement(2503..3465)
                     /locus_tag="PK264_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRD78669.1"
                     /translation="MIPATLVQSTAETLMAKAAIEIPEDYLGGLRACAKSEKGDLSSF
                     VIQAMLENYEAAKEDRRAMCGDTGVPRWYVKLGNEAAIAGGPIALEGALRRATANATR
                     AVPLRPNRVHPLWRTDHDNNVGINAPEIEYAFHPDADWIELTTVHKGGLFGSDYRMLF
                     PGDGIDGIKRFYIDTLIAFGKRGLACQPAIVGIGLGGSKDTCMVLGKQASCLRVVGSR
                     NPDPKIAALEDELKTLGNSLGMGAMGFMGSSMVIASHIEVGYCHTGGMPMSVHMFCLS
                     SRRATARIHRDGRVDFRTDPQWFTDYMRRATVGWGGEAPAVAAE"
     gene            complement(3576..4151)
                     /locus_tag="PK264_22485"
     CDS             complement(3576..4151)
                     /locus_tag="PK264_22485"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRD78670.1"
                     /translation="MSYHSSEQLEHPGHLTPVDQPIGIDRVLLPRLMFASGRTSKAVA
                     RIRGIEANPHSVALGLAIGVFAAFVPLFGLQLGLAATGAWFLGANVVAALIGTFIGNP
                     LTWPLMWVASYKVGTVLLGREAATADLASTIDRIGATVVSLSPGTLSETGQLLRPILE
                     ALTVGSIPLGLLVAGLAYLIARRLLLGAGAT"
     gene            complement(4295..>4761)
                     /locus_tag="PK264_22490"
     CDS             complement(4295..>4761)
                     /locus_tag="PK264_22490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HRD78671.1"
                     /translation="AGGTRSDAVLPIRGLNNDVPVVFEPGGAVPGDRIVGVMIPGKGI
                     RVFQIHSPRLSEFENQSWIDVTWDIDPEKPERFPAKLMVTALNEPGTLAQIAQAVADR
                     DGNIDNVRMMRRAADFTEMLFELEVWDLGHLNEIIQAVRSKVVVSKVERVFD"
BASE COUNT          870 a         1517 c         1593 g          781 t
ORIGIN      
        1 ccgtcgtcgc catgcccgtg ggcgacgagc aggtcgcggg ctggctcgcc gcactcgacg
       61 agatggaggc agtgctcgac ggcaggaagc tgctgccgca ctggcggctg tcgcagggct
      121 tcgatctgaa gcaggtgctc gaggagccaa agccgttcga tctggtgctc tggatcacgg
      181 gccacgcggc gctgccctac accaagccgg gtgagaccgt ctcttcggcg acctgggcca
      241 actggcagcg cgtgttcggc ggcaatttcc tcgcctatgc cttctggttc aactgacgcc
      301 gtctcactcg ctgagcgtcg tcaccttgag ccgctggtcc tcgagcggca gcccgagtag
      361 cgcctggatg aagcgcgcga agaattggag cgcaagcagc aggatcccga aggttacgat
      421 ggcctgcggg atccacagcc gcgtgtcgct cgagatcgac gtctggccac ggctgaaggc
      481 ctcgtaggtg aagcggaaca tcgcgtaggt gagaaagcct gccatcatga gaccggccgc
      541 cgaaacgagc acctcggccg cacggcgaag tggcacgggc aggcgcgcaa ggagcagcgt
      601 cacgcggata tgcccgcccg agcgcagcgt catcgccgcg ccgaacgtga acgtcgccgc
      661 catcaggtag gagctgtatt cccaggcgac gggaatatcg gccgggaccg atggaaagta
      721 gtgcgagagc gccctgacgg tgatctcggc gaggatcagc agcgtgagaa gagcgaggca
      781 gacggccgcc atccaaccat cgatccggca caaggcgtcg atgccgtcga gcaggatgcg
      841 gatggggcca ggggctgagc ggttcgggct atggcccacc tctgcgtcac ccgccatgcc
      901 cacgctccaa ctccgccggc cttcgatcag taggccgacg ctgtcgggcc aactccgtcc
      961 ttgttgtaga gatcgcggcg gaactggaca acgccgtcct ccgtcgccca ggcggtgatg
     1021 taggcgaagt agagcgggat cggcttcttg agcttcacgt cgagccgctc accggacttc
     1081 ttcatctcca tgacccgctc gggcgtccag ccctggtcct gcatcagcca ggccgcgacg
     1141 tgctcgatcc ccgagatgcg cacgcagccc gagctcgccg cacggaaatt gcggccgaac
     1201 agggtgtgcg agggcgtgtc gtgcatgtag accgagtgcg agttgtggaa gttgatcttc
     1261 acgaagccga gcggattttc agggcccggc gtctgcttga aggagagtga gcgcgccatc
     1321 gaccagttga cgcgcagggg atcgatcttg cggccgtccg gcccgtaggc gtcgatgccg
     1381 tacttggcga gcacgttctg gccgcgccgc gacatctcca tgcccttcgg gatcaggtcc
     1441 ttttcgatca ccgtcggcgg gaggtgccac accggattga agttgagctc gtggatctgc
     1501 gatttcagga tcggcgtctg gcgatcgatc ttgccgacga caccggcatg gcgcgagacg
     1561 acgatatcgc cttcgaccac ctcgatctgg gcggctggga tgttgaccat gatgtagcgg
     1621 ccgccggggc ggctgaactc gctcagtcgc gtcaggttga ggcggagctg tcggagccgc
     1681 gccgctgcgg gcacgttgag cgccgcgatc gtgcgtttgt cgacgatgcc gttgggggtg
     1741 aggccgttgg aggcctggaa acggcggacg gccttctcga catagccgtc gaaggcatcg
     1801 gagatggcaa aacccgcttc cttgagatcg cccgacatca tgaggcgctg gcgaagcacg
     1861 ccgacggccg ggtggttcat gccgatctga agcggcacct cgggaatgac gggaaagccg
     1921 cccttggcaa cgacatctgc gtaaagctgg atcgcggcct tcatcggacc gaggttgcgc
     1981 gcggagagtg tcgcgaagcc tttcggcggc ttggcctccc actccttggc aaagctgcgg
     2041 tcggtgtggt ccgcgcttgc gccgtacgtg gcatagcctg gcgccgcgga cccgaaccgc
     2101 atccagccac tgctgccgtc gtatgaagac gctccagttg ccgcaaggca gcttgccagg
     2161 ccgacggcga gcgctccgag cacgccccgg ccaacgatcc ctctttcaag cacgtcaggc
     2221 actcccgctg agaaggcgcg actcaatcca gcgccgtcgg ccaacgcctt ttggcgcaga
     2281 gttaggccga atggttaaca cgacgtgaac accgcgcccc cgcagatgca gcgcacgcaa
     2341 gacactccga atcacgcggt cagcgcatct ggagcaaccg cgacgaaaat gaggccgcga
     2401 ggcctgattg tgaagtcgcg gaactgcaac agctgccgga tcggcaagtg atgatgccga
     2461 ccggcaacaa tgaactgcga tcaaattgag cagaatggca aattattccg cagcgaccgc
     2521 cggcgcctcg ccgccccatc cgaccgtcgc gcgacgcatg tagtcggtga accactgcgg
     2581 gtccgtacgg aaatccacgc ggccatcgcg atgaatgcgg gccgtcgcgc gccgcgacga
     2641 gaggcagaac atgtggacgg acatcggcat gccgccggtg tggcaatagc cgacctcgat
     2701 gtggctcgcg atcaccatcg acgagcccat gaagcccatc gcgcccatgc cgagggagtt
     2761 gccgagcgtc ttcagctcgt cctcgagtgc ggcgattttc ggatcgggat tgcgcgagcc
     2821 gacgacgcgc aggcacgagg cctgcttgcc gagcaccatg caggtatcct tggagccgcc
     2881 gagtccgatg ccgacgatgg ccggctggca ggcgaggccg cgcttgccga aggcgatcag
     2941 cgtgtcgatg tagaagcgct tgatgccgtc gatgccgtca cctggaaaga gcatgcggta
     3001 gtcggagccg aagaggccgc ccttgtgcac ggtcgtgagc tcgatccagt cggcgtcggg
     3061 gtggaacgcg tactcgatct cgggcgcgtt gatgccgacg ttgttgtcgt ggtcggtccg
     3121 ccagagcgga tggacgcgat tgggcctcag cggcactgcg cgcgtcgcat tggccgtcgc
     3181 ccgccggagc gccccttcga gcgcgatcgg gccaccggcg atcgccgcct cgttgccgag
     3241 cttcacgtac cagcgcggca cgcccgtatc gccgcacatg gcgcgccgat cctccttcgc
     3301 cgcctcgtag ttctcgagca tcgcctggat gacgaacgag gaaagatcgc ccttctcgct
     3361 cttggcgcaa gcgcggagcc caccgagata atcctcgggg atctcgatgg cggccttggc
     3421 catcaaggtt tcggcggtcg actggacgag ggtcgcgggg atcatggcga ggctcgcggg
     3481 atgaggtcgg atctccggca tttctgccag ccaattcaag cccgaggcaa gcgccagtta
     3541 ttgcgaatgc ggcgcgccgc gcaaccattg cccgctcagg tcgcgccggc gccgagcagc
     3601 aggcggcgtg cgatcaggta agcgagcccc gcgaccaaga ggccgagcgg gatgctgccg
     3661 acggtcaagg cctcgagtat gggccgcagc aactgccccg tctcgctcag ggtgccgggc
     3721 gacaggctca cgaccgtcgc gccgatgcga tcaatggtcg acgcgagatc ggcagtcgcc
     3781 gcctcacggc cgagcagcac ggtcccaacc ttgtaggagg caacccacat caacggccag
     3841 gtcaacggat tgccgatgaa ggtgccgatc agtgctgcga ccacattcgc cccgagaaac
     3901 caggcgcctg tcgccgcgag cccgagctga aggccgaaga ggggtacgaa tgccgcgaaa
     3961 acgccgattg cgagcccgag cgccacggaa tgcgggttgg cctcgatgcc gcggatccgc
     4021 gccacggcct tgctggtccg tccgctggca aacatcaacc gcgggaggag aacccgatcg
     4081 atcccgatcg gctgatcgac gggcgtcaaa tgtcccggat gctcgagctg ctcgcttgaa
     4141 tgataggaca aggggccgag tcccgcatga ggtgccacgc tcgacgccac tacgatcgct
     4201 ggccacgtga aaggccagca gcaagggtga tcaaaacgct gaaatccggc gacaagggag
     4261 cgcgacgctc agtagcccgc gattcgccac cgcttcagtc aaacactctc tcgactttgc
     4321 tgacaacgac tttcgagcgc actgcctgga tgatctcgtt gaggtggccg agatcccaca
     4381 cctcgagctc gaacagcatc tccgtgaaat cggcggcacg ccgcatcatg cgcacgttgt
     4441 cgatattgcc gtcccgatcg gcgacggcct gggcgatttg ggcgagcgtg cccggctcat
     4501 tcagcgcggt caccatcagt tttgccggaa agcgctcggg cttctcgggg tcgatgtccc
     4561 aggtgacgtc gatccagctc tggttctcaa attcggagag cctcggggag tggatctgga
     4621 agacgcggat gcccttcccc gggatcatca cgccgacgat gcggtcacca ggcactgccc
     4681 cgcccggctc gaagacgacc gggacatcgt tattgaggcc gcggatgggc agtaccgcgt
     4741 cgctgcgggt gccgccagca c
//
LOCUS       DAORNH010000331         4752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13370_length_4752_cov_2.552480, whole genome shotgun sequence.
ACCESSION   DAORNH010000331 DAORNH010000000
VERSION     DAORNH010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13370_length_4752_cov_2.552480"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PK264_22495"
     CDS             complement(<1..251)
                     /locus_tag="PK264_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HRD78672.1"
                     /translation="MLFALAAAAGAKDAGGMPQFNPEHFVPQLFWLIVSFTLLYLLLS
                     RIALPRIGQVIEERQDRVRRDLDEANRMKSDTEKALAAY"
     gene            complement(395..>538)
                     /locus_tag="PK264_22500"
     CDS             complement(395..>538)
                     /locus_tag="PK264_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HRD78673.1"
                     /translation="FGNFLSGALRNPSAADGQRLWMFVGAAMAELLGLLAFVVAMVLL
                     YAK"
     assembly_gap    539..555
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(669..>816)
                     /locus_tag="PK264_22505"
     CDS             complement(669..>816)
                     /locus_tag="PK264_22505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRD78674.1"
                     /translation="GLGLWGGWAPLAFMVGFTGFEVMVAILQAFIFTLLTCIYLNDAL
                     HMHH"
     assembly_gap    817..841
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<842..1388)
                     /locus_tag="PK264_22510"
     CDS             complement(<842..1388)
                     /locus_tag="PK264_22510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRD78675.1"
                     /translation="MHQFEIERFVPLNLLGYDISFTNSSLWMLIGVGTVAGFLILSMR
                     GRSLVPTRMQSMAEIVYEFVADTVRSTCGEHGMKYFPIIFTLFSFILISNLLGLLPKS
                     FTVTSHIIVTLALAVGVFLFVTFLGFKENGIGYLKMFVPSGVPALLLPLIVLIEVVSY
                     LMRPISLSVRLFANMLAGHVLL"
     gene            complement(1554..1952)
                     /locus_tag="PK264_22515"
     CDS             complement(1554..1952)
                     /locus_tag="PK264_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRD78676.1"
                     /translation="MSSQDHTRDPGRGEISPEDREAMRRRAADIGRRLDEVKGSQGPV
                     KHEAAAGSSLGYAFRFTFELLVGLLVGGGVGWWLDQQFGKAPLFMIVLAMLGFVGALV
                     NVIRAAQKDKATREASLRPAPSVKDDDEDR"
     gene            complement(2337..2786)
                     /locus_tag="PK264_22520"
     CDS             complement(2337..2786)
                     /locus_tag="PK264_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRD78677.1"
                     /translation="MADEQPTSDALAARLKTAGLRPTRQRIALGELLLNGPDRHVTAE
                     GLHAEALAAGESVSLATVYNTLHQFVGVGLLRELALNGDKAYFDTNVSNHSHFFDERS
                     GRVTDIDDEAIRVEGVPIPPAGMEISHVDVVVHLRPASSFPRRKTTD"
     gene            complement(2927..>4752)
                     /locus_tag="PK264_22525"
     CDS             complement(2927..>4752)
                     /locus_tag="PK264_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRD78678.1"
                     /translation="CFIIEERLKQLLDIPVFHDDQHGTAIIAAAGLINAIELTGRDLK
                     TTKLVVNGAGAAGIACLDLLVAMGRPRANVTFCDTKGVIYQGRKEGMNQWKSGYAAKT
                     KARTLADALDGADAFFGLSAKGAVTPEMVKSMAKNPIIFAMANPDPEITPEEVAATRS
                     DAIVATGRSDYPNQINNVLGFPFIFRGALDVRASTINTEMKIAAAKALAELARAEVPD
                     QVAAAFHGRRPTFGPDYIIPAPFDPRLITNIPPAVAKAAIETGVARKPIDINGYVARL
                     SGRLDPVAGWLQSIFGEVRKAPRGIVFTEGEEDVVVRAANAYLNLGFGTPTLVGRSDR
                     INTAFKNAGIKLRDGIRVINARESEHNAEYADYLYQRLQRRGFLRRDCQRMVNQDRNV
                     FAACMVALGHADGMITGVTRNWTTAFTEVSRVLDVKAGHRVIGVSIAICRGRTVLVAD
                     TSVVELPTGQELAEIAIEAARVARRFGMTPRVAMLAYANFGNPPGERSQHVRDAVALL
                     DQRGVDFEYEGEMAADVALNKEAMKLFPFCRLSDTANVLVMPAFHSASISTKLLQELG
                     GATIVGPMLVGLSHSVQICPLGAKDSDIVNMAALAAYNVTP"
BASE COUNT          897 a         1481 c         1530 g          802 t
ORIGIN      
        1 tcgtaggccg ccagagcctt ttccgtgtcg ctcttcatgc ggttcgcctc gtcgaggtcg
       61 cgccgcaccc ggtcctggcg ctcctcgatt acctgcccga tacggggcag ggcgatgcgg
      121 gagagcagca agtagagcag cgtgaacgag acgatcagcc agaagagctg cggcacgaag
      181 tgttcgggat tgaactgggg cattccgccc gcgtccttgg ccccggcagc agcggcaagc
      241 gcgaaaagca tcgtcgactc caagaaggcg tcagcggtaa gcaaccgtac gctccggcag
      301 tgcggccgga cacgaacgac gagagcgcgc gggcacccgc acgctctcta tatgcgccgg
      361 ctgatccaag acggctcgag ccggccgcca gaactcactt cgcgtagagc agaaccatcg
      421 cgacgacgaa ggcgagcagg ccgaggagct cggccatggc ggcgccgacg aacatccaca
      481 gacgctggcc gtcggcggcc gacgggttgc gcagcgcgcc cgagaggaag ttgccgaann
      541 nnnnnnnnnn nnnnnatgag gcccgtgcag gcgatgccgg cggcgagaag ctttgcgact
      601 tccggggtca tgcgtgtctc cttggcatgt atgcgagtgt tggttgacga acgtccacag
      661 agcccggctc agtggtgcat atggagggca tcgttgaggt agatgcaggt gagaagcgtg
      721 aagatgaacg cctgcaagat ggcgaccatc acctcgaagc ccgtgaagcc gaccatgaac
      781 gcgagcggcg cccagccgcc ccagagcccg aggccgnnnn nnnnnnnnnn nnnnnnnnnn
      841 ntgaggagca cgtggccggc aagcatgttg gcgaacagtc ggaccgagag gctgatcgga
      901 cgcatcaggt aggacacgac ctcgatcagc acgatgagcg gcaagaggag cgccggcacg
      961 cccgagggca cgaacatctt gagatagccg atgccgtttt ccttgaagcc gaggaaggtg
     1021 acgaacagga acacgccgac cgcgagcgcg agtgtcacga tgatgtggct cgtcaccgta
     1081 aaggacttcg gcagcaggcc gagcaggttc gagatcagga tgaacgagaa cagcgtgaag
     1141 atgatgggga agtacttcat cccatgctcg ccgcatgtac ttcgcacggt atcggcgacg
     1201 aactcgtaga cgatctcggc catcgactgc atgcgggtcg gcacgagcga gcggccgcgc
     1261 atggacagga tgaggaagcc ggcgacagtg ccgacgccga tcagcatcca gagcgaggag
     1321 ttggtgaacg aaatgtcgta gcccagcaga ttgagcggca caaaccgctc gatctcgaac
     1381 tggtgcatcg gtccgtgatc ttgtcccgcc acgtctgcct ctcgaagccc tgcgacccgt
     1441 ccttcaacgc gtcccgtctc gctctcgtac gaccggagcc agtgctccgg tcaacccgca
     1501 aaccgcaaac cgaaagtcca caatccttcg aaccacaccg attcgagccg ccgtcaccga
     1561 tcctcgtcgt cgtccttgac tgagggcgcg ggacgcagcg aagcttcccg cgtggccttg
     1621 tccttctggg ccgcgcggat gacgttcacg agagcgccga cgaaaccgag catcgccagc
     1681 acgatcatga acaacggcgc cttgccgaac tgctgatcga gccaccagcc gacgcctccg
     1741 ccgacgagca ggccaaccag caattcgaag gtgaagcgaa acgcgtagcc gagcgacgat
     1801 cccgccgcgg cctcgtgttt gacaggtcct tgcgaaccct tcacctcgtc gagcctccgg
     1861 ccgatgtcgg cggcgcgccg cctcatagcc tccctatcct ccgggctgat ctcacctcgg
     1921 cccggatcgc gcgtgtgatc ttggctcgac atcagggctc ccggggaggg caaacggcaa
     1981 ggcgggcatc gacgcggcgc accatagtga cggtgccgcg gggtgtcaag aaaccatatg
     2041 ataggcttaa gtcgcagaaa cataaggaat ttattcgagg catctcgcct tgcgacaata
     2101 cggcttgctg gcgctttcag gacaatcagt ggtcggaatc cgccttctcg gcttcaacac
     2161 agtgaaaggt cggatttacc tgaaaatccc tgctctcaga accttatgcc catcgtcgaa
     2221 agaaccctgg tcatccgaat acgccacgcg gtgagatggg caaagggtcc catcgaccgc
     2281 cccgaatggc cttgcctttg gtatcggaac gacatcggac acgaaaccga cgaggatcag
     2341 tcggtcgtct tccgccgtgg gaaagaggaa gccgggcgca ggtgaaccac cacgtccacg
     2401 tggctgatct ccatgcctgc gggaggtatc ggaacaccct cgacgcggat tgcttcatcg
     2461 tcgatatcgg tgacgcgccc cgaccgctcg tcgaagaaat ggctgtggtt cgacacgttg
     2521 gtgtcgaagt acgccttgtc gccattgagc gcgagttcgc gcagcaggcc caccccgacg
     2581 aactgatgca gcgtattgta gaccgttgcg agcgacacgc tctcacctgc cgccaacgct
     2641 tccgcgtgca acccttcagc tgtcacgtgc cgatcgggac cgttgagcag aagctcgccg
     2701 agcgcaatgc gctgccgcgt cgggcgcaaa cccgcggttt tcaaccgagc cgcgagagcg
     2761 tcgctcgttg gttgctcgtc tgccatcgcc tcagtctgcg ctcgtccgcc ttacgtccga
     2821 tagaccggcg acccggtggc cgggcgccaa ctgatatatt aaccgcccaa tcggtcttgc
     2881 gcaaccgatc cgagccaacg gcttctcgca gcagggcccc gctccgtcaa ggcgtgacgt
     2941 tataagccgc cagcgccgcc atgttgacga tgtcgctgtc cttggcgccg agcgggcaga
     3001 tctgcaccga atgcgacaag ccgaccagca tcggcccgac gattgtcgcc ccgccgagct
     3061 cctgcaggag cttcgtcgag atcgaagctg aatggaaggc cggcatcacg agcacgttcg
     3121 ccgtgtcgct gagccggcag aacgggaaga gcttcatggc ctccttattg agcgccacgt
     3181 ctgctgccat ctcgccttcg tactcgaagt cgacgccgcg ctggtcgagg agtgcgaccg
     3241 catcgcgcac gtgctgcgag cgctcgccgg gaggattgcc gaagttggca taggcgagca
     3301 tggcgacgcg cggcgtcatg ccgaaacggc gcgcgacgcg tgcagcctcg atggcgattt
     3361 ctgcgagctc ctggcccgtc ggcagctcga cgacactcgt atcggcgacg agcacggtgc
     3421 ggccgcggca gatggcgatc gatacgccga tgacgcggtg tccggccttc acgtcaagca
     3481 ctcggctgac ctcggtaaag gcggtcgtcc agttgcgtgt gacacccgtg atcatgccgt
     3541 cggcgtggcc gagggcgacc atgcaggcag cgaacacgtt tcggtcctgg ttcaccatgc
     3601 gctggcagtc gcggcgcaga aagccacggc gctgcaggcg ctgatagagg tagtcggcgt
     3661 attcggcatt atgctcgctc tcgcgcgcgt tgatcacgcg gatgccgtcg cgcagcttta
     3721 tgcctgcatt cttgaaggcg gtgttgatgc gatcgctgcg accgaccagc gtcggcgtgc
     3781 cgaagccgag attgaggtag gcgttggccg cgcggacgac gacatcctcc tcgccctccg
     3841 tgaagacgat gccacgcggc gccttgcgca cctcgccgaa gatcgattgc agccagcccg
     3901 cgacaggatc gaggcggccc gagaggcgtg ccacgtagcc gttgatatcg atcggcttcc
     3961 gcgcgacgcc cgtctcgatc gcggccttgg cgacggccgg cgggatattc gtgatcaggc
     4021 gcggatcgaa gggcgccggg atgatgtagt caggtccgaa ggtcgggcgg cggccgtgga
     4081 aggctgccgc cacctggtcg ggcacttcgg cgcgggcgag ttcggcgagc gccttggccg
     4141 cggcgatctt catctcggtg ttgatggtcg acgcgcgcac gtctagcgcg ccgcggaaga
     4201 tgaaggggaa gcccagcacg ttgttgatct ggttcggata atccgagcgt cccgtggcga
     4261 cgatcgcatc cgagcgcgtc gccgcgacct cctcgggcgt gatctcgggg tcggggttcg
     4321 ccatggcgaa gatgatcggg ttcttcgcca tcgacttcac catctccggc gtcaccgcgc
     4381 ccttggccga gaggccgaag aaggcgtcgg cgccatcgag ggcatcagcg agcgtgcgcg
     4441 ccttggtctt cgcagcgtag cccgacttcc actggttcat accctcctta cgaccctggt
     4501 aaatcacccc cttggtatcg cagaaagtga cgttggcgcg gggacggccc atggcgacga
     4561 gaaggtcgag gcaggcgatc ccggctgccc ctgcgccgtt gacgacgagc ttcgtcgtct
     4621 tgagatcgcg gcccgtgagc tcgatagcat tgatgaggcc cgccgccgct atgattgcgg
     4681 tgccgtgctg gtcgtcgtgg aagaccggga tgtcgaggag ttgcttcagg cgctcctcga
     4741 taatgaagca at
//
LOCUS       DAORNH010000332         4733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13448_length_4733_cov_2.626122, whole genome shotgun sequence.
ACCESSION   DAORNH010000332 DAORNH010000000
VERSION     DAORNH010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13448_length_4733_cov_2.626122"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK264_22530"
     CDS             <1..219
                     /locus_tag="PK264_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78679.1"
                     /translation="ATATTQPTRRHGVMPDQTREVLRRIGQALRLAAGGEPATTPPPP
                     ADRRAEAGNVTAPSRAAAAPAASATKQP"
     assembly_gap    283..328
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <329..830
                     /locus_tag="PK264_22535"
     CDS             <329..830
                     /locus_tag="PK264_22535"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HRD78680.1"
                     /translation="ERGSRLTTRTFGSWVYWPGAARFDADPYTRAHFARRGELPHSPA
                     LAMTFQARIDSDGNRLHSACEYEVAGPDINSVWWSLAVYDDRGTLIPNPAGRHAYNAN
                     STLRAANGSFAVVLSREARPGNWLPTSGAGPIVLVLDVQEPRGGWPAPGTEIAALPVI
                     KKVACR"
     gene            827..1399
                     /locus_tag="PK264_22540"
     CDS             827..1399
                     /locus_tag="PK264_22540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78681.1"
                     /translation="MSTLVVRRPRWPAIALALVLGAIIHIVATLAVPHIAATTAYRRL
                     SGNLPVNEMRLLARPTPSGQSFPFQSPDVRVAVCRFDIGNAPLSVSAVLPERGWTLTI
                     YSRHGDSVHAIAAQEERSTDIGFRLVQAPEPSLLSMITGGRPVATDTTQVNSPVRDGL
                     IVIRAPVRSASFLPAVEAVLGAARCEPEAQ"
     gene            1410..2459
                     /locus_tag="PK264_22545"
     CDS             1410..2459
                     /locus_tag="PK264_22545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRD78682.1"
                     /translation="MRVHFCIPGDITLPTGGYAYDRRILQGLLAQGVDARHVELPETY
                     PAPDAAALADTLRATSSAESGDVLLIDGLAFGAMPAELVTVMATGGRHIVALVHHPLC
                     LEAGITSADAARLRASERTALALATAVIVSSPMTARTLVTDFDVPPGKITVAEPGTDR
                     ALRAPRRGDPPRLLAVGSVVPRKAYGVLVEALSALIGLPWRLTIAGAARDAAEFSRVR
                     QVIEGHGLAARIELLGAVAEERIPGLYAEADIFVMPSLYEGYGMALAEAMARGLPIVC
                     TTGGAAAETCPDAAAIKVPPGDVVAFREALRRVLGNAPLVAAMSDASFAAGHALPTWD
                     DTVRSIVGALSRIAR"
     gene            2456..3298
                     /locus_tag="PK264_22550"
     CDS             2456..3298
                     /locus_tag="PK264_22550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD78683.1"
                     /translation="MSFSPEWLALREPVDHRSRSTKLATRLAARLGARDRIDVVDLGC
                     GAGSNIRATAPLLGRDQRWTLVDYDPRLLAVARERLLAWGRPISESGDTIAIERIDGG
                     IARHLAVTFREADLARDLDRALGERPKLVTAAAFFDLVSAEFIGRLAAAVAARGADFY
                     TTLTFNGVQTWEPRHTADSHMLQSFCRHQHGDKGFGPAAGPDAPAILAKEFAIDGYRI
                     SEGDSPWHIGPGDGTLLADLAAGFASAVAERRDVPAAAIAAWRSISRTGAVVGHTDTL
                     ATIG"
     gene            3365..4246
                     /locus_tag="PK264_22555"
     CDS             3365..4246
                     /locus_tag="PK264_22555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HRD78684.1"
                     /translation="MPHAQTRDWPGHMPAAGAAVRQSFGHFGPLEVRLAVKEAEIEAA
                     QRLRYEVFYDEMGAVPTQEARRLRLDRDHYDAVCDHLLVIERVGAAGAAERIVGTYRL
                     LRRDVAARVGLPLYTSTEFDLSPLLAGSGARLRLMELGRSCVARGKRDRRTLEALWHG
                     IWAYVRSHGIEMMIGCASFPGVDPAKHRRPLDYLHRHHLAPHELRCRALTDRYVSLTA
                     SDGAGGADERATLRALPPLVKGYLRLGAFVGDGAVVDHAFGTTDVLVILPVERIDPRY
                     FDHFGAPEGTDTRLRQG"
     gene            4345..>4733
                     /locus_tag="PK264_22560"
     CDS             4345..>4733
                     /locus_tag="PK264_22560"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRD78685.1"
                     /translation="MSGEIEIRVADEVQLIRLTRADKKNALTAGMYEAMSAALDRGEA
                     DAGIAVHVFMGAGGTFSAGNDISDFVRRAKGAGATGIPTPSLEFIRRLPKVTKPMIAA
                     VDGLAIGVGTTMLLHCDLVYAGDNAAFS"
BASE COUNT          702 a         1621 c         1586 g          778 t
ORIGIN      
        1 gccaccgcta cgacacagcc gacgcgccgg cacggtgtga tgcccgacca gacccgggag
       61 gtgctgcggc gcatcggcca ggctctgcgg ctcgcggccg gcggcgagcc cgcgacgaca
      121 ccaccgccac ccgctgatcg gcgcgcagaa gccggcaacg tgaccgctcc gagccgcgcg
      181 gccgccgcgc ctgccgcatc ggcgacgaag cagccctgag gcccgtgagc cctggcggcg
      241 ggaggctgac atgctgttga atctggtgct cgtcctggta ctnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg agcgcggcag ccgcctcacg accaggacgt
      361 tcggctcgtg ggtctactgg ccgggcgccg cccgcttcga cgccgatccc tacactcgcg
      421 cgcatttcgc ccggcgcggg gagcttccgc attcgccggc gctcgcgatg acgttccagg
      481 cgcgtatcga cagcgatggc aatcgcctgc actcggcctg cgagtacgag gtcgcagggc
      541 cggatatcaa ttccgtgtgg tggagcctcg cggtgtacga cgatcgcggc acgctgatcc
      601 cgaacccagc cggtcgccac gcctacaacg cgaactcgac gctcagggcc gcaaacggga
      661 gctttgcggt cgtgctctca cgggaggcgc ggccgggcaa ctggctgccg accagcggcg
      721 ccgggccgat cgtcctcgtt ctcgacgtgc aggagcctcg cggcggctgg cccgcaccgg
      781 gtaccgagat cgcggcgttg cccgtcatca agaaggttgc ctgcagatga gcacgctcgt
      841 tgtccgacgg ccacgctggc cggcgattgc cctggccctc gtgctcggtg ccatcatcca
      901 catcgtcgcg acgctcgcgg tgccccatat cgcggcaacc acggcctatc ggcggctctc
      961 aggaaacctg ccggtcaacg agatgcggtt gctcgcgcgg ccaacgccat ccggccagtc
     1021 cttccccttc cagtccccgg acgtacgtgt cgccgtctgc cgctttgata tcggcaatgc
     1081 gccgctctcc gtctctgccg tgctcccgga gcgcggctgg acgctgacga tctactcgcg
     1141 tcatggtgac agcgtgcatg cgatcgctgc tcaggaggag cgatcgacgg acatcggctt
     1201 ccggctggtc caggcaccgg aaccgtcgct tctctcaatg atcaccggag gccgaccggt
     1261 cgcgaccgac acgacacagg tgaactcgcc agtgcgagac ggattgatcg tgatccgggc
     1321 gccagtgcga agcgcctctt tcctgcccgc ggtcgaagca gtgctcggtg ccgcgcgctg
     1381 cgagcctgag gcgcagtgac gggaaagaca tgcgagttca tttctgcatc ccaggtgaca
     1441 tcacgctccc gaccggcggc tatgcctatg atcgtcgcat tctgcagggc ctgctggcgc
     1501 agggcgtgga tgcacggcat gtcgagctgc cggagaccta tcccgctccg gacgccgcgg
     1561 cgctggctga cacactgcgt gctacgtcat ccgcagaatc gggcgatgtg ctcctgatcg
     1621 acggcctcgc ctttggagcc atgccggccg agctcgtcac agtcatggcg acgggggggc
     1681 ggcacatcgt ggcgctcgtc catcatccct tgtgcctcga agcagggatc acttctgctg
     1741 acgcggcgcg gctcagggcg agcgaacgga cggcgctcgc cctcgcaaca gctgtgatcg
     1801 tttcgagccc gatgacggcg cggacgctgg tcaccgactt cgacgttcca cccggcaaga
     1861 tcacggtcgc cgagcccggc acggaccggg cgttgcgtgc gcctagacgc ggcgacccgc
     1921 ctcgcctgct ggcggtcggc tctgtcgtgc cgcggaaggc ctacggcgtg ctggtcgaag
     1981 cgttgtcggc cctcatcggg ctgccgtggc gtttgaccat tgccggggca gcacgcgatg
     2041 ccgccgagtt ttcccgcgtg cggcaggtga tcgaggggca tgggctggcg gcccgcatcg
     2101 aattgctcgg cgcggtcgcc gaagagcgca taccggggct ctatgcggag gcggacatct
     2161 tcgtcatgcc gtcgctctat gaaggctacg gcatggcgct cgccgaagcc atggcgcgtg
     2221 gcctgccgat cgtctgcacg accggaggcg ctgcggcgga gacttgcccg gacgccgccg
     2281 ccatcaaagt gccgcccgga gacgtcgtcg ccttccgcga ggcattgcgt cgcgtgcttg
     2341 gcaatgctcc gctagtcgcg gccatgtcgg acgcgtcgtt tgccgctgga cacgcgttgc
     2401 cgacgtggga cgacaccgtc cgttcgatcg tcggcgcgct ttcgaggatt gcccgatgag
     2461 cttctcgccc gagtggctcg cactgcgcga gcccgtcgac cacaggtcgc gatcgaccaa
     2521 gctcgcgaca cgcctcgccg cgcgcctcgg cgcacgagac cgtatcgacg tcgtcgacct
     2581 cggctgcggc gccggctcca acatccgcgc gacggcgccg ctgctcggcc gcgatcagcg
     2641 ctggaccctg gtcgactatg atccgcgatt gctcgcagtc gcccgcgagc ggctgctcgc
     2701 ctgggggcgc ccgatctcgg agagcggcga taccattgcc atcgagcgca tcgatggcgg
     2761 tatcgcacgg caccttgccg tgacgtttcg ggaagctgac ctcgcgcgcg atctcgatcg
     2821 ggcgctcggc gagcgtccga aactcgtcac ggccgccgct ttcttcgatc tcgtctcggc
     2881 cgagttcatc gggcggcttg ccgcggccgt cgcggcgcgc ggcgcggact tctacacgac
     2941 cctcaccttc aatggtgtcc agacgtggga gccgcggcac acggccgatt cccatatgct
     3001 gcagtcattc tgccggcacc agcacggcga caagggcttc gggccggccg cgggaccgga
     3061 tgcaccggcg atccttgcga aggagttcgc aatcgacggc tatcgcatat cggaagggga
     3121 cagcccttgg cacatcgggc ccggcgatgg cacgctcctc gccgatctcg ccgccgggtt
     3181 cgccagcgcc gtcgccgagc gccgggacgt tccggccgca gcaattgccg cctggagatc
     3241 gatctcgcgg acaggcgccg tcgtcgggca caccgatacg ctggcaacga tcggttgatt
     3301 gtcgcgaagt tgataccttg ggcaggtaag cctccacgac cacgcgatca cgatggggtt
     3361 tcaaatgccg cacgcacaaa cgcgcgattg gccgggacac atgccggctg ctggtgcggc
     3421 cgtgcgacaa tcattcgggc atttcggccc gctcgaggtc cggctcgccg tcaaggaggc
     3481 tgagatcgag gcggcgcagc ggctgcgcta cgaggtcttc tacgacgaga tgggcgctgt
     3541 tccgacgcag gaggcgcggc gcctcaggct cgacagggat cactacgacg ccgtctgcga
     3601 tcacctgctg gtcatcgaac gcgtgggcgc agccggtgcc gcggagcgca tcgttggcac
     3661 ctacaggctg ctccggcgag atgtcgcggc gagggtggga ctgccactct acacgtcgac
     3721 cgaattcgat ctttcgccgt tgctcgcggg cagcggcgcg cgcctccgat tgatggagct
     3781 tggccgctcg tgcgtggcgc gcggcaagcg ggatcggcgc acgctcgagg cgctctggca
     3841 cggcatctgg gcctatgtcc gctcgcacgg catcgagatg atgatcggct gcgcgagctt
     3901 ccccggcgtc gaccccgcca agcatcgccg gccgctcgat tacctgcatc ggcatcatct
     3961 ggcgccgcac gagctgcgct gccgggcgct taccgaccgt tacgtgtccc tgaccgccag
     4021 cgatggggct ggcggcgccg acgagcgagc cacgcttcgc gctctgccgc cgctggtgaa
     4081 gggatacctg aggctcggcg cctttgtcgg tgacggagct gtcgtcgatc acgccttcgg
     4141 cacgacagac gtcctcgtca ttctcccggt tgagcggatc gacccgcgct acttcgacca
     4201 tttcggggcg ccggagggca ccgacacacg gctgcgacaa ggttagcaag cccgggcgct
     4261 caacctcgct ctcaacctcg ccatcgacct cgcgctgaat gttcgctagc ctctgagcag
     4321 gcatgtgttg gcagggggac agcgatgagc ggtgagatcg agatcagagt agccgacgag
     4381 gtgcagctca tccgcctgac gcgggctgac aagaagaacg cgctgaccgc cggcatgtac
     4441 gaggcgatgt cggcagcgct cgaccggggc gaggcggacg ccgggatcgc cgtgcacgtc
     4501 ttcatgggcg ccggcggcac cttctcagcc ggcaacgaca tcagcgattt cgtgcgacgt
     4561 gccaaggggg cgggggcgac cggcatcccg acgccctctc tcgagttcat ccgccgcctg
     4621 ccgaaggtca cgaagccgat gatcgccgcc gtcgatggcc tggcgatcgg cgtcggcacc
     4681 accatgctgc tgcactgcga cctggtctat gccggcgaca acgcggcgtt ctc
//
LOCUS       DAORNH010000333         4731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13457_length_4731_cov_2.370402, whole genome shotgun sequence.
ACCESSION   DAORNH010000333 DAORNH010000000
VERSION     DAORNH010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13457_length_4731_cov_2.370402"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PK264_22565"
     CDS             <1..818
                     /locus_tag="PK264_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78686.1"
                     /translation="ENSGRRALELDPSDLWAAHAVAHVLEMQGRRTEGIEHLKSLEPH
                     WDGGNHFMHHLWWHLGLYHYERGEYDEVLRLYDTKYRNHHSALNKAVPDLYVDFQNAA
                     SMLFRLERQGVDVGSRWIEIAEHAEKRIGDNRSPFTQTHWMMALAATNRWDVAERMLA
                     SMRAYGESEPGTLGRLVKRISLPICEAIVARGKGDHRRAVELMRPVLGTMFQIGGSHA
                     QQDVLEQLFLDSAEKAGSNDAVRLLIERVTARHPVPPERRAGYRTSAARIGAT"
     gene            complement(856..1413)
                     /locus_tag="PK264_22570"
     CDS             complement(856..1413)
                     /locus_tag="PK264_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRD78687.1"
                     /translation="MFIQTEPTPNPATLKFLPGKPVLGDGTADYRAPAEASLSPLAQK
                     IFAVDGVKAVFLGTDFISVTKGSAEWAHLKPAILGAIMEHYMSGAPVIASGEGDDDVP
                     QGYYDPKDEEVVKTIKELLETRVRPAVANDGGDIVFHGFKDGVVSLQMRGACSGCPSS
                     TATLRHGIENLLKHFCPEVQEVRPV"
     gene            complement(1641..2150)
                     /locus_tag="PK264_22575"
     CDS             complement(1641..2150)
                     /locus_tag="PK264_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRD78688.1"
                     /translation="MPKKRRSFEQGHRRKYLVIVDESQEVESALYFAASRIGHSVGGG
                     IKLLYVIEPHADYTHWVGVRQVQIEEEQAKAKALFRLFRMKLTTGGFDNVVVEEDVRE
                     GKKVETILKVIDEDEDIGILVLGAAVDNRGPGPLVASLATGKFAGTFPIPVVIVPGEL
                     KLEDITSMA"
     gene            complement(2243..3148)
                     /gene="rimK"
                     /locus_tag="PK264_22580"
     CDS             complement(2243..3148)
                     /gene="rimK"
                     /locus_tag="PK264_22580"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HRD78689.1"
                     /translation="MKLVMLARNPGLYSHKRIIDAARARGHTIDVINTLHVHMKITSN
                     QPVLRHGGKTLPIYDAVIPRIGASITSYGLAVLRQFEVQGVYPLNESVAIGRSRDKLR
                     ALQLLAREGIGLPVTAFAHDPRRAEDVIQEVGGAPVVIKLLEGTQGMGVILAETDASA
                     KSIIEAFSAANISIMVQEFVREAGGSDVRALIIGGKVVAAMKRSGRKGDFRSNLHRGG
                     SSEPVELSEAEVRTAVRSAEVLGLNVCGVDMLRSGRGPLVMEVNSSPGIEGMERASGI
                     NVADAMIAFLEAKARRGDTGTKGTG"
     gene            complement(3176..4030)
                     /locus_tag="PK264_22585"
     CDS             complement(3176..4030)
                     /locus_tag="PK264_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HRD78690.1"
                     /translation="MSKPIVAIIAAGNMGAAVAARLTQAGVTVLTSLVGRSSASEERA
                     RVAGMRAASDAEIASADVILSVLPPRDAMGLVKRLSPAIKSSGRKPIYVDGNAVSPAT
                     VEQIGAVATEAGARFVDGAIIGPPPKADGSSRTILYVSGEHADQVLPLGKLGIEVRKV
                     AGGVGAASALKMSYAAVTKGLTALGTASLIAASEAGVAEELRRELEESQPAVLAFLTR
                     GVPDMCPKAYRWVNEMEEVGTYTGRPAGNQIYKGIAALYQGIADDMAGGRTDVRTLED
                     FFTSAKRR"
     gene            complement(4120..>4731)
                     /locus_tag="PK264_22590"
     CDS             complement(4120..>4731)
                     /locus_tag="PK264_22590"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HRD78691.1"
                     /translation="LRDFVSNNQMASGAAMGGLGAVVLGTETGRRMAARAAKLGALAL
                     IGGLAYKAYQNYSKGQPALGGPSTHGLIEAAPAGSGFEPQAITNETATLYIRAMIAAA
                     AADGRIDEGEQERILGSLKQAGVEQGAFEFLEQEVQNPASIDDLAGAVTSPEVAVQVY
                     AAARMSIDPDTGEEQGFLTELASKLGIDADLAQHIETLAAGSA"
BASE COUNT          771 a         1604 c         1547 g          809 t
ORIGIN      
        1 ctgaaaattc cggtcggcgc gcactcgagc tcgatccgtc cgatttgtgg gccgcacacg
       61 ccgtcgccca cgtcctcgaa atgcagggcc ggcggacgga gggcatcgag cacctgaaga
      121 gcctcgagcc gcactgggac ggcggcaatc acttcatgca tcacctgtgg tggcatctcg
      181 ggctctacca ctacgagcgc ggcgagtacg acgaggtgct ccgcctctac gacacgaagt
      241 accgcaacca ccattcagcc ctcaacaagg ccgtgccgga cctctacgtc gatttccaga
      301 acgcggcctc gatgctgttc cgcctggagc ggcagggcgt cgacgtcggc tcacgctgga
      361 tcgagatcgc cgagcatgca gagaagcgca tcggcgacaa ccgctcgccc ttcacgcaga
      421 cgcactggat gatggcgctc gcggcaacca atcggtggga cgtggccgag cgcatgctcg
      481 cctcgatgcg tgcctatggc gagagcgagc cgggtacgct cggccggctc gtcaagcgca
      541 tctcgcttcc catctgcgaa gcgatcgtcg ctcggggcaa gggcgatcac cgccgagccg
      601 tcgagctgat gcggcccgtg ctcggcacca tgttccagat cggcggcagc catgcgcagc
      661 aggacgtgct cgagcaactc ttcctcgaca gcgcggagaa ggccggctcc aacgatgccg
      721 tccgcctgct gatcgagcgg gtgaccgcgc gccatcccgt gccgcccgag cggcgcgcgg
      781 gctataggac gtcggcggcg cggatcgggg ccacctgatc gcgcaagccg cacatcatcc
      841 aaccggccca gcccttcaaa ccggccggac ttcctgaacc tcggggcaga agtgcttcag
      901 aaggttctcg atgccgtggc gcagcgtcgc cgtcgagctc ggacagccgg agcaggcgcc
      961 tcgcatctgc agcgagacga cgccgtcctt gaagccgtgg aagacgatat cgccgccgtc
     1021 attggcgacg gccggacgga cgcgcgtctc caggagctcc ttgatggtct tcacgacctc
     1081 ctcgtccttc gggtcgtagt agccctgcgg cacgtcgtcg tcgccctccc cgctggcgat
     1141 gacgggcgcg cccgacatgt agtgctccat gatcgcgccg aggatcgccg gcttcaggtg
     1201 cgcccactcg gcgcttcctt tggtgacgga gatgaaatcc gtgccgagga acacggcctt
     1261 cacgccgtcg accgcgaata tcttctgcgc gagcggggag agcgaggcct cggcgggtgc
     1321 gcgatagtcg gccgtcccgt cgccgagcac gggcttgccc ggcaggaact tgagcgtcgc
     1381 gggattgggt gtcggctcgg tctggatgaa catgcgcgcc tccacgggtt tcgtcgctaa
     1441 cgtgggagcg atacccgatt atttggaatc attctaggtg gattacaagg tctgcgcaca
     1501 gcgtctccgc tgtgtccgag tggggaggga agcatggccg ccctgcggcc agagcgacca
     1561 gccccgcaca ccgtcgggag gcagagcctc ccgaacccac cactttttgg gatgcagacg
     1621 tgcggcgagc gggcggggca ctacgccatc gaggtgatgt cctcaagctt cagctcgccg
     1681 gggacgatca cgaccgggat cgggaaggtg ccggcaaact tgcccgtagc gagtgatgcg
     1741 acgagcgggc ctggtccacg attgtcgacc gcggcgccga gcacgaggat gccgatgtcc
     1801 tcgtcttcgt cgatcacctt gaggatggtc tcgacctttt tgccctcgcg cacatcctcc
     1861 tcgacgacga cattgtcgaa gccgccggtg gtgagcttca tgcggaagag gcggaacagc
     1921 gccttggcct tcgcctgctc ctcctcgatc tgcacctgcc gcacaccgac ccagtgcgtg
     1981 taatcggcat gcggctcgat cacatagagc agcttgatgc cgcctccgac ggaatggccg
     2041 atgcgcgagg ccgcgaagta gagagcgctc tccacttcct ggctctcatc gacgatcacc
     2101 agatatttgc gccggtgacc ctgctcgaaa cttctgcgtt ttttaggcat ggtaccagtc
     2161 tggcatgttc gcacgggcgc aacaatggcc gacgtgcgcg tcgcgaatag ttgtactcag
     2221 gtcgagacga aacgctggcc gctcatccag tgcccttcgt gcccgtatcg ccgcgccgtg
     2281 cttttgcctc gaggaaggcg atcatggcat cggcgacgtt gatgccgctc gcccgctcca
     2341 tgccctcgat gccgggcgag gagttgacct ccatgacgag cggcccgcgc ccggaacgga
     2401 gcatgtcgac gccgcagacg ttgagaccga gcacctcggc cgaacggaca gccgtgcgca
     2461 cttcggcttc gctgagctcg accggctccg agctgccgcc gcgatgcagg ttcgagcgga
     2521 agtcgccctt gcggcccgag cgcttcatgg ccgcgaccac tttgccgccg atgatcagtg
     2581 cgcgcacatc gctgccgccc gcttcgcgca cgaactcctg caccatgatc gagatgttgg
     2641 ccgcgctgaa ggcttcgata atcgacttcg ccgaggcgtc ggtctcagcg aggatcacgc
     2701 ccatgccctg cgtgccctcg agcagcttga tgacgaccgg ggcgccgccg acctcctgga
     2761 tgacgtcctc ggcccggcgg ggatcgtgcg cgaacgccgt cacggggagc ccgatgccct
     2821 ctcgcgccag caattgcaga gcgcgcagct tgtcgcgcga gcggccgatg gcgacgctct
     2881 cgttgagagg atagacgccc tgcacctcga actggcgcag cacggcgagg ccgtagctag
     2941 tgatggaggc cccgatgcgc gggatcaccg catcatagat cggcagcgtc ttgccgccgt
     3001 gccgcagcac gggctggttg gacgtgatct tcatgtggac atgcagcgtg ttgatcacat
     3061 cgatcgtgtg accacgcgcg cgcgccgcat cgatgatgcg cttgtgcgag tagaggcccg
     3121 gattgcgagc cagcatgacg agcttcatcg gcatggtctg ccctcgcctg cccccttagc
     3181 ggcgcttcgc tgatgtgaag aagtcctcga gggtccgcac gtctgtccgc ccgcccgcca
     3241 tatcgtcggc aatgccctgg tagagcgctg cgatcccctt gtagatctgg ttgcccgcgg
     3301 gccggcccgt gtaggtgccg acctcctcca tctcgttcac ccagcgataa gccttagggc
     3361 acatgtctgg cacgccccgc gtcaggaagg cgagcaccgc cggctggctc tcttcgagct
     3421 cgcgccgcaa ctcctccgcg acgcctgcct cggatgcggc gatgagcgag gccgtgccga
     3481 gcgcggtcag ccctttcgtc accgccgcat aggacatctt gagagcggac gccgcgccga
     3541 caccgccggc gaccttgcgc acctcgatgc cgagcttgcc gagcggcagc acctgatcgg
     3601 catgctcgcc gctgacatag agaatcgttc gcgacgagcc atctgctttt ggcggcggcc
     3661 cgatgatcgc gccgtcgacg aagcgcgcgc cggcctccgt cgcaaccgcg ccgatctgct
     3721 cgactgtcgc cgggctcacg gcattgccat cgacatagat cggcttgcgg cccgaggact
     3781 tgatcgccgg ggaaaggcgc ttgacgagcc ccatggcgtc gcgtggcggc agcaccgaga
     3841 gaatgacgtc agctgaggcg atttcggcgt cgctcgctgc acgcatgcct gccacgcggg
     3901 cgcgctcctc gctcgccgat gagcgaccca caagcgacgt caggaccgtc acgccggctt
     3961 gcgtgaggcg cgcggcaacg gcggcaccca tgtttccggc cgcgatgatg gcgacgatcg
     4021 gtttcgacat gctgataggc tcccggaaac agcgacggac ggggagcgct ccccgtccgt
     4081 ttttttgggc cctatcggac ccgctctgtc aagcgtgcgt caggcgctgc ccgccgccag
     4141 cgtctcgatg tgctgggcga gatcagcgtc gatgccgagc ttcgaagcga gctcggtgag
     4201 gaagccctgc tcctcgcccg tgtcgggatc gatcgacatg cgggccgccg cgtagacctg
     4261 tacggcgacc tcaggcgagg tgacggcgcc ggccagatca tctatcgagg ccgggttctg
     4321 cacctcctgc tcgaggaact cgaaggcacc ctgctcgacc cccgcctgct taagcgagcc
     4381 gaggatgcgc tcctgctcgc cctcgtcgat gcgcccgtcg gcggctgcgg cggcgatcat
     4441 cgcgcggatg tagagcgtgg cagtctcatt ggtgatcgcc tgcggctcga agccggaacc
     4501 agcgggcgcc gcctcgatga gaccatgagt cgacggtccg ccgagcgcag gctggccctt
     4561 cgagtagttc tggtaggcct tgtaggcgag gccgccgatg agggcgagcg caccgagctt
     4621 ggccgcacgt gccgccatgc gccgcccggt ctccgtgccg agcacgacgg cgccgagacc
     4681 gcccatggcc gcacccgacg ccatctggtt gttgctcacg aagtcacgaa g
//
LOCUS       DAORNH010000334         4723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13483_length_4723_cov_2.253856, whole genome shotgun sequence.
ACCESSION   DAORNH010000334 DAORNH010000000
VERSION     DAORNH010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13483_length_4723_cov_2.253856"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>439
                     /locus_tag="PK264_22595"
     CDS             <1..>439
                     /locus_tag="PK264_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRD78692.1"
                     /translation="VRRRAKAINFGIIYGISAFGLAAQLGISKQEAGEYIQTYFKRFP
                     GIRDYMERTKAEAHAQGFVTTIFGRKIHYPEINTKNPSMRGFLERAAINAPIQGSAAD
                     IIRRAMIRMPGALEGKKLGATMLLQVHDELIFEVPAGEVDKKLP"
     assembly_gap    440..449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <450..535
                     /locus_tag="PK264_22600"
     CDS             <450..535
                     /locus_tag="PK264_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78693.1"
                     /translation="TSAEPAVKLSVPIKVDAKAADNWEAAH"
     gene            560..>918
                     /locus_tag="PK264_22605"
     CDS             560..>918
                     /locus_tag="PK264_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78694.1"
                     /translation="MRLRHLISTVAALGICASAAPARAFDALERLLPAVAPGAAFEQQ
                     MLFTYQGFPGQPPADYLDALAKIGAAGTGGLDQFTLMALKDPTDALRLTGIDLSKARG
                     WLVSGNPPADISAVLGT"
     assembly_gap    919..928
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <929..1728
                     /locus_tag="PK264_22610"
     CDS             <929..1728
                     /locus_tag="PK264_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78695.1"
                     /translation="AGSALEARKFEKSTVRTFPVWSFGEDGANNLARRDIHDPFGRGL
                     GRAQRIALLGDRVVISAQTDVLDPALVALNRPRLCAMCPTYSAILGEAKRNAGGKAEV
                     LAATGLGVQIPLTDAELSAKIAGVKSDDEAGRVIAEFIQKRASRVTMPGYRVAVLLAT
                     RIGTGGVAQIGLAYANEEDARKGVPIIAKRLAAETIGALGVFSASIGKERPPQKATET
                     RIVEKDGEWIGIITIRYEGDWTIAFKEFKSWKNLVLSRSFSVLSILD"
     gene            1811..2635
                     /locus_tag="PK264_22615"
     CDS             1811..2635
                     /locus_tag="PK264_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD78696.1"
                     /translation="MIPRSILITGCSSGIGLAAARTLKSRGWRVIATARKDEDLARLE
                     RDEGVEAIPLELADQASIEACTSDALKRTDGKLLALYNNAAYGVVGAMEDTPATLLRD
                     HFQVNVIAANELARRLIPAMRANRTGRIVNCSSVLGFVSGPYRGAYSASKFALEAMTD
                     AMRLEVRDAGIHVSLLEPGPIATSFMATTIATFRRAIDMEGSVHRDYYRRRIAELEAG
                     GGRFRTGPETVVAKLVHALESPRPKARYRISLPTHAAAFMKRVLPSSVLDAVVARF"
     gene            complement(2669..3106)
                     /locus_tag="PK264_22620"
     CDS             complement(2669..3106)
                     /locus_tag="PK264_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78697.1"
                     /translation="MNRQFWTAVAAYVVPTFPLGYFWHLSIFKAQYDTLALYRAEVII
                     PLGLAAMLTPGLLFAWLYPRLFSTAPGDWLKSALRFFLVFGALAWSFLVLPVAAKYNM
                     TSVGRFIALETAFTILQYAVTSPLIAWAWRGGEGRAASDHRLA"
     gene            3190..3633
                     /locus_tag="PK264_22625"
     CDS             3190..3633
                     /locus_tag="PK264_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD78698.1"
                     /translation="MVTTSGPLRSHCPINFGLEIFGDKWTLLVLRDLLLKGKRSFKEF
                     QSSEEGIASNILAERLARLERTGLVTSARVESDSRQIHYLPTTDCRALLPLLVEMAYW
                     GARHDPRTAAPEDFVQAYERDRDALVLAMAEVGAQSAGAAQSVRE"
     gene            3707..3907
                     /locus_tag="PK264_22630"
     CDS             3707..3907
                     /locus_tag="PK264_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78699.1"
                     /translation="MLKIAALVWVMLGTTLAGIAMTAILSVPQLAEQSTRLIPMLCSA
                     GFVLAMPISYLVAKKIASTTSR"
     gene            complement(3988..4461)
                     /locus_tag="PK264_22635"
     CDS             complement(3988..4461)
                     /locus_tag="PK264_22635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD78700.1"
                     /translation="MATAKPENTIIMETTQGRVVIELRPDLAPGHVERIKLLAREKFY
                     DGIVFHRVIDGFMAQCGCPRGEGTSGSKHPNLTAEFNMEPHVRGTCSMARRQDPDSAN
                     SQFFICFGPAPSLNRQYTVWGKVVEGMENVDKLKRGEPVRNPDKIISMRVAADLA"
     assembly_gap    4466..4492
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4493..>4723)
                     /locus_tag="PK264_22640"
     CDS             complement(<4493..>4723)
                     /locus_tag="PK264_22640"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRD78701.1"
                     /translation="SEFSRYVFKRGTVGMARARSPHSANSQFFICFDNDGCRDLSGSY
                     TVWGQVIEGMEHVDRITVGEPPATPDIIVRMQ"
BASE COUNT          856 a         1500 c         1512 g          808 t
ORIGIN      
        1 ggtacggcgg cgcgcgaagg ccatcaattt cggcatcatc tacggcatct cggcgttcgg
       61 cctcgccgcc cagctcggca tctccaagca ggaagcaggc gaatacatcc agacctactt
      121 caagcgcttc cccggcatcc gcgactacat ggagcgcacg aaggcagagg cacacgccca
      181 gggcttcgtc acgacgatct tcggtcgcaa gatccactat cccgagatca acacgaagaa
      241 cccctcgatg cgcggcttcc tggagcgggc cgccatcaat gcgccgatcc agggctcggc
      301 cgcggacatc atccgccgcg ccatgatccg catgccggga gcgctcgagg gaaagaagct
      361 cggcgcgaca atgctgcttc aggtgcacga tgagctcatc ttcgaagtgc cggcgggcga
      421 ggtcgacaag aagctccccn nnnnnnnnna gacatccgcc gagccggcgg ttaagctcag
      481 cgtgccgatc aaggtcgacg ccaaggccgc cgacaactgg gaagcggcgc actgacgtca
      541 tccacggaga tttctggaaa tgcggttgcg acacctgatt tcgacggttg cagcacttgg
      601 catctgcgcc tcggcagcgc ctgcccgcgc cttcgatgcg ctggagcgcc tgttgccggc
      661 cgtggcacct ggggctgcgt tcgagcagca gatgctgttc acataccagg gctttccagg
      721 gcagccgccc gcggactacc tcgatgctct ggcgaagatc ggcgccgctg gtaccggagg
      781 cctcgatcag ttcacgctca tggcgctgaa ggacccgacg gacgcactcc gcctcactgg
      841 catcgacctc tccaaggcgc gcggctggct cgtctctggc aatccgccgg ccgatatcag
      901 tgccgtgctg gggaccaann nnnnnnnngt gcgggtagcg cattggaagc gcgcaagttc
      961 gagaagtcga cggtgaggac ctttccggtc tggtccttcg gcgaggatgg cgcgaacaac
     1021 ctggccagac gcgacatcca tgatccgttc ggccgcggtc tcgggcgcgc ccagcgcatc
     1081 gcgctgcttg gcgatcgcgt ggtaatcagt gcgcaaaccg acgtgctcga tccggccctg
     1141 gtcgcgctca acaggccccg tctgtgcgcc atgtgcccga cctattcggc cattcttggc
     1201 gaggccaagc gcaatgccgg cggcaaggct gaagtcctgg ctgctacggg gcttggcgtg
     1261 caaattccgc tcacagatgc tgaactttct gcaaagattg cgggcgtgaa gtcggatgac
     1321 gaggccggcc gggtcatcgc ggaattcatc caaaagcgtg cgagccgcgt caccatgccg
     1381 ggctatcgcg tggccgtcct gcttgcgacc cggatcggga ccggaggcgt cgcccagatc
     1441 ggactggcct atgccaacga ggaggatgct cgcaagggcg tgccgatcat cgccaagcgt
     1501 ctcgcggccg agacgatcgg agccttgggt gtcttcagcg cctccatcgg caaggagcgc
     1561 ccgccgcaga aggccacgga gacccgtatc gtcgagaagg atggcgagtg gatcggcatc
     1621 atcaccatcc gctacgaggg tgattggacc atcgccttca aggaattcaa gtcttggaag
     1681 aatctggtgc tcagccgctc gttctcagtt ctgagcatcc tggactgact attgtcgcaa
     1741 gcgccttgga caagcacccg gagatcgcgc caagtgcggc ctcatcgcgg gcaacattcg
     1801 gcaaggtctc atgattcctc gttcgatcct catcaccggc tgctcctccg gcatcggact
     1861 tgccgccgca cgcacgctga agtcgcgcgg ctggcgcgtc attgctacgg cgcgcaagga
     1921 cgaggacctc gctcggctcg agcgagatga gggggtggag gcgatcccgc tcgaactcgc
     1981 cgatcaggca tcgatcgagg cctgcacttc ggacgctttg aagcgcacgg acggcaagct
     2041 cctggccctc tacaacaacg cggcctacgg cgtcgtcggc gccatggagg atacccccgc
     2101 cacgcttctt cgcgatcatt tccaggtcaa tgtgatcgca gcgaacgagc tcgcgcggcg
     2161 gctgatcccg gcaatgcgcg ccaaccggac ggggcggatc gtcaactgct cgtccgtgct
     2221 cggcttcgtg agcgggcctt atcgtggcgc ctattccgcc tccaagttcg cgctcgaagc
     2281 gatgacagac gccatgcggc tcgaggtgcg cgatgcaggc atccacgtct cgctgctgga
     2341 gcccggcccg atcgcgacga gcttcatggc gacgacgatc gccacgtttc gccgcgcgat
     2401 cgacatggag ggcagcgtcc accgcgacta ctaccgccgc cgcatcgctg agctcgaggc
     2461 cggcggcggg cgcttccgca ccggccccga gaccgtcgtc gccaagctcg tccatgccct
     2521 cgaaagcccg cggcccaagg cgcgctatcg catctcgctg cccacccacg ccgcggcctt
     2581 catgaagcgc gtgctgccga gctcagtgct cgacgcggtc gtcgcgcgct tttgacgggg
     2641 gcaggtcgcg gccgacgccg accaccggtt aggccagccg atgatcgctc gccgcgcggc
     2701 cctcgccccc gcgccacgcc caggcaatca gcggcgacgt cactgcatac tgaaggatag
     2761 tgaaggctgt ttcgagggca atgaacctgc cgaccgacgt catgttgtat tttgccgcga
     2821 ccggaaggac caggaaggac caggcgagag caccaaatac gaggaaaaac cgcagggcgc
     2881 tcttgagcca atcaccgggc gcggtgctga acagtcgcgg gtagagccag gcgaagagca
     2941 ggcctggcgt cagcatcgcc gccagtccca agggtatgat aacctccgcg cgatagagcg
     3001 cgagtgtgtc gtactgcgcc ttgaagatcg agagatgcca gaaatagccc aggggaaatg
     3061 tcggcacgac ataggctgcc acagcggtcc agaattgcct gttcataacc gtgccttccg
     3121 ttattacttg catattacaa gtgataccag gatgagagac agccttcaag tggtgagcgc
     3181 gagccaacaa tggtgacgac gtcagggccc ttgcgatcac attgcccgat caatttcggg
     3241 ctcgaaatct tcggcgacaa gtggaccttg ctcgtgctcc gcgacctgct gctgaagggc
     3301 aagcgaagct tcaaggaatt ccagtcgtcg gaggaaggca tcgctagcaa catcctcgcc
     3361 gagcggctgg cacggctcga gcgtaccggc ctcgtgacat cggcgcgggt cgagagcgat
     3421 agccgccaga tccactatct gcccacaacc gattgccggg cgctgctgcc gctgctggtc
     3481 gagatggcct actggggggc acggcacgat ccgcggacgg cggcacccga ggacttcgtg
     3541 caagcctacg agagggaccg cgatgctctc gtcttggcca tggcggaggt gggcgcacaa
     3601 tccgcggggg ccgcgcaatc cgtacgagaa tgatgcgagc tttgacgccc atcaatgcgg
     3661 cggcgaggcc tgtgggcgac gattggtcgt accgatggag gctcgtatgc tcaagatcgc
     3721 agcactcgtc tgggtcatgc tcggcacgac gctcgcaggc atcgccatga ccgcgatcct
     3781 cagtgtgccg cagctcgccg agcagagcac gcggctgatc ccaatgctgt gctcagccgg
     3841 gttcgtactg gcgatgccga tctcctatct agtcgccaag aagatcgcct cgaccacctc
     3901 gcgctgagcg ggaggcccaa tgaatcgcgc cgcgctcggg tctgccgggc gcggcgtctt
     3961 cgtggccggg aacaacctcg ataagggtca ggcaaggtcg gcggcgacgc gcatggagat
     4021 gatcttgtcg gggttgcgca cgggctcgcc gcgcttcaac ttgtcgacgt tctccatgcc
     4081 ctcgacgact ttgccccaca ccgtgtactg gcgattgagc gacggcgccg gcccgaagca
     4141 gatgaagaac tggctgttgg cgctgtcggg atcttggcgg cgtgccatgg agcaggtgcc
     4201 gcgcacgtgc ggctccatgt tgaactcggc ggtcagattc gggtgcttgg agccgctggt
     4261 tccttcgccg cgcgggcagc cgcactgggc catgaagccg tcaatcacac gatggaagac
     4321 gataccgtcg tagaacttct cgcgggccag cagcttgatg cgctcgacgt gaccgggggc
     4381 gagatccggg cgaagctcga tgacgacgcg gccctgcgtc gtctccatga tgatggtgtt
     4441 ctctggtttg gcggtggcca tgattnnnnn nnnnnnnnnn nnnnnnnnnn nncctgcatg
     4501 cgcacgatga tatcgggcgt cgcgggcggc tcgccgacgg tgatgcggtc gacgtgctcc
     4561 atgccctcga tcacctggcc ccagaccgtg taggagccgg agagatcgcg gcagccgtcg
     4621 ttgtcgaagc agatgaagaa ctggctgttg gcgctatgcg ggctcctcgc ccgcgccatg
     4681 ccgacggtgc cgcgcttgaa gacataacgc gagaactccg act
//
LOCUS       DAORNH010000335         4712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13523_length_4712_cov_2.885763, whole genome shotgun sequence.
ACCESSION   DAORNH010000335 DAORNH010000000
VERSION     DAORNH010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13523_length_4712_cov_2.885763"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PK264_22645"
     CDS             <1..742
                     /locus_tag="PK264_22645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD78702.1"
                     /translation="NATLFHQEYSDFQLNSFLGTSFVVRSIPDLTRFRRLPADPGLKK
                     GRRHLVGYVGIMGAQDGVDLLVRAMHELVHHDGRRDVQCLIAGSGTELDALKRLAHVL
                     EVEDYVTFTGFLSGEPLLTAFSTFDIGVIPDPKNVYNDKISMNKVFEYMTLGIPFVGF
                     DLIEGRQTAADAALYAADVTPACLAGRMAQLLDDDTLRASMAATGLDRARRLLSWDAE
                     RAQLLAAYELALRPRLEAAGAAAAKPIR"
     gene            complement(825..>1563)
                     /locus_tag="PK264_22650"
     CDS             complement(825..>1563)
                     /locus_tag="PK264_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRD78703.1"
                     /translation="KAVTKGYAIGSAGLGALVLFAAYTEDIKHFAAKAKPGTLFHNMV
                     PDFSLSNPYVVVGLLFGGLLPYLFGGIAMTAVGRAAGSIVEEVRRQFKAKPGIMKGTE
                     KPDYAKAVDLLTVAAIREMIVPSLLPVLSPIVCFFAVKWMAGTANAFAALGAMLLGVI
                     VNGLFVAISMTSGGGAWDNAKKAFEDGFVDKDGVKHMKGSDAHKASVTGDTVGDPYKD
                     TAGPAVNPMIKITNIVALLLIAVLAGH"
     assembly_gap    1564..1585
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1586..2963)
                     /locus_tag="PK264_22655"
     CDS             complement(<1586..2963)
                     /locus_tag="PK264_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HRD78704.1"
                     /translation="MDSMMWFIIVAGLLSLVYGVVTVKAVLSSSAGNAKMQEIAAAIQ
                     EGASAYLTRQYTTIAIVGAVVTVIVGLLLKWQVAVGFVIGAVLSGIAGFIGMWVSVRA
                     NVRTAQASTVSLADGLSMAFRAGAVTGLLVAGLALLGVAVYFFLLTRMGGFPATDRTV
                     VDSLVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYAVSIVATMVLASIYFAAKITDATLLMVYPLAICGLCVITS
                     IIGTFAVRLGSNQSIMGALYKGIIATGVLSLIALWPLTEYMLGMKTTFTPAGGAPFTG
                     QDLFWCGAVGLVATAAIIVVTEYYTGIGYRPVRSISQASVTGHGTNVIQGLAVSLEAT
                     ALPAIIIIAGILVTFKLAGLFGIAIAVTTMLALAGMIVALDAFGPVTDNAGGIAEMSG
                     LPKDLRRTT"
     gene            3718..4590
                     /locus_tag="PK264_22660"
     CDS             3718..4590
                     /locus_tag="PK264_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78705.1"
                     /translation="MAQSLGDLSHDARSPVPASLRLLMAAEAFFVLVAGATLYCLPAE
                     TNKYFAWTIQPSLTAAMLGAAYVAAFALQVSVLRQARMIEARVGMPAVSLFATLGLAA
                     TLLHIDRFHFQSPEPTARLFAIVWLLVYVLVPIVSVWLLRPWWRALWQNVPGREPLAG
                     AMFWNLMILGMMLSLAGIIMLLSPQGVIPNWPWRLTPLTARMLGAWSVGIGFALIQAA
                     RLGDEAQVRPAAPAIIVLSVLSLIALARFSDQVAWGTVTAVVILVVLINLFVVAVIAV
                     ARPMNNRAARGLAQ"
     gene            4593..>4712
                     /locus_tag="PK264_22665"
     CDS             4593..>4712
                     /locus_tag="PK264_22665"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HRD78706.1"
                     /translation="MRPHAAVAGAGMAGLLAARVLAEAGWRVSLVERDRLDGAP"
BASE COUNT          782 a         1574 c         1535 g          799 t
ORIGIN      
        1 caacgccacg ttgttccacc aggaatactc ggacttccag ctcaacagct tcctgggcac
       61 cagcttcgtc gtgcgctcga tcccggacct tacacgcttc cgccggctgc cggccgatcc
      121 cgggctcaag aaggggcgtc gccacctcgt cggctatgtc ggcatcatgg gcgcgcagga
      181 cggcgtcgat ctgctcgttc gcgccatgca cgaactcgtc catcacgacg gccgccgcga
      241 cgtgcaatgc ctgatcgccg gctcgggtac cgagctcgat gccctgaagc gcctcgccca
      301 cgttctcgag gtcgaggact acgtgacctt cacgggcttc ctctcgggcg agccgctgct
      361 caccgccttc tcgaccttcg acatcggtgt cattcccgat ccgaagaacg tctacaacga
      421 caagatctcg atgaataagg tgttcgagta catgacgctc ggcatcccct tcgtcggctt
      481 cgacctcatt gagggacgcc agaccgcggc tgacgcagcc ctctatgccg cagatgtgac
      541 gccggcctgt ctcgccgggc gaatggcgca gctccttgac gacgacacgc tccgcgcctc
      601 gatggccgca acaggcctcg atcgcgcccg ccgcctcctg agctgggacg cggagcgcgc
      661 gcagctgctc gccgcctacg agctggcgct ccgccctcgc ctcgaggctg ccggtgccgc
      721 cgcggcaaag ccgatcaggt agcggctcga cgacatgccg agtgcacaac agcgaagggc
      781 gccctcgggc gcccttcgac agtttaggag atgggaaccc gccgtcagtg ccccgcgagg
      841 accgcgatga gcaggagcgc cacgatgttt gtgatcttga tcatcgggtt cacggcgggg
      901 ccggcggtgt ccttgtaggg atcgccgacg gtgtcaccgg tcactgacgc cttgtgcgcg
      961 tccgagccct tcatgtgctt cacgccgtcc ttgtcgacga agccatcctc gaacgccttc
     1021 ttggcgttgt cccaggcgcc gccgccggat gtcatcgaga tggcgacgaa caagccgttg
     1081 acgatcacgc ccaagagcat cgcgccgagt gcggcaaagg cattggcggt gccggccatc
     1141 cacttcacgg cgaaaaagca gacgatcggc gaaagcacgg gcagcagcga gggtacgatc
     1201 atctcgcgaa tggcggcaac cgtgaggagg tcgacggcct tggcgtaatc gggcttctcg
     1261 gtgcccttca tgatgcccgg cttcgccttg aactggcggc gcacctcctc gacgatggag
     1321 cccgccgcgc ggccgacggc cgtcatggcg atgccgccga agaggtaggg gaggagaccg
     1381 ccgaacagga gaccgaccac cacgtagggg ttcgagagcg agaagtccgg caccatgttg
     1441 tggaacagcg tgccgggctt cgccttcgcc gcgaagtgct tgatgtcctc ggtgtaggcc
     1501 gcgaacagga cgagcgcgcc gagaccggcc gagccgatcg cgtagccctt ggtcaccgcc
     1561 ttcnnnnnnn nnnnnnnnnn nnnnncggtc gtgcggcgga ggtccttcgg caggccgctc
     1621 atctcggcga tgccgccggc gttgtccgtg accgggccga aggcgtcgag cgcgacgatc
     1681 atgccggcga gcgccagcat cgtcgtcacc gcgatggcga tgccgaagag gccggcgagc
     1741 ttgaaggtga ccaggatgcc cgcgatgatg atgatcgcgg gaagtgccgt cgcctcgagc
     1801 gacacggcga gtccctggat cacgttggtg ccgtggccgg taaccgaggc ctggctgatc
     1861 gagcggaccg gacgatagcc gatgccggtg tagtactcgg tgacgacgat gatcgccgcc
     1921 gtcgcaacga ggccgacggc gccgcaccag aacaggtcct ggccggtgaa cggggcgccg
     1981 cccgccggcg tgaacgtcgt cttcatgccg agcatatact cggtgagcgg ccagagcgcg
     2041 atcagcgata gcacgcccgt cgcgatgatg cccttgtaga gcgcgcccat gatcgactgg
     2101 ttcgagccga ggcggacggc aaacgtgccg atgatcgagg tgatcacgca gaggccgcag
     2161 atcgccagcg gatagaccat gaggagcgtc gcgtcggtga tcttggcggc gaagtagatc
     2221 gaggccagca ccatggtggc gacgatcgac accgcgtagg tctcgaacag gtcggccgcc
     2281 atgccggcgc agtcgccgac gttgtcgccg acgttgtcgg cgatggtcgc agggttccgc
     2341 ggatcatcct cggggatgcc ggcctcgacc ttgccgacga ggtcgccgcc gacgtcggcg
     2401 cctttcgtga agatgccgcc gccgagacgg gcgaagatcg agatcagcga ggcgccgaag
     2461 ccgagggcga cgaggctgtc gacaacggtg cggtcggtcg ccgggaagcc acccatgcgg
     2521 gtcagcagga agaagtagac ggcgacgccg agaagcgcga ggcctgcgac gaggagccct
     2581 gtcaccgcgc cggcgcggaa ggccatggag aggccgtcgg cgagcgacac cgtggaggcc
     2641 tgcgccgtgc gcacgtttgc acggaccgac acccacatgc cgatgaagcc ggcgatgccc
     2701 gagagcaccg cgccgatgac gaagccgacc gccacctgcc acttgagcag gagcccgacg
     2761 atcactgtga ccactgcgcc gacgatcgcg atcgtcgtgt actggcgcgt gagataggcg
     2821 gatgcgcctt cctggatcgc cgcggcgatt tcctgcatct tggcgttgcc ggcgctcgac
     2881 gaaagcacgg ccttcaccgt gacgacgccg tagacaagcg acaacagccc ggcgacgatg
     2941 atgaaccaca tcatggaatc catggcggca gagcctcccc ccggtgagtg aaagtgacga
     3001 cggaccacgc ctgaagcggc ggcagccccc ttggccccgc cgctctagca ccatcaggca
     3061 ccagaagcac gctccgcgct gactgacttc gactgaagtt caaaacgcgg ccggaccatg
     3121 ccaaaactgt cacgccgttg caaccgcgac acattggcgg gggatggctg cggggggact
     3181 gcggcgcttc tcggcgcggc ttctccccgc cgattgcggg cgccccggac ctgcgcatca
     3241 ttcgcgctcc gtcgactccc cgtcggggct gctacgacgc aggttggcgc tgagcggagc
     3301 gttgcccaag aggccgggaa cgaccgtcaa agatggactt cacaagtgct caatccccca
     3361 cattgtcttg gtcgggctcg tcccaaccac ccatcgggcc gcatgctgcg aggcctgtcg
     3421 atggatgaga cctcgagcca agctgaagga cggcaatcgc agacgtgagc gcgaattcgg
     3481 cgtcctgcgc acgcaacagc actcccgttc ctgaattcgg cggacgtggc gcacttggtc
     3541 gagtaccggg atcgttccca ctgcttgcgt gcggcatagg tacagcagca gagcctggac
     3601 gcggcccgcc attcacctcg tctggcgctc aactgaccgg tcgatgacgc tacaattgac
     3661 cgcccgattc ggcccatgcc aagatcgccg aaggcattca ttggatacgg aagcgatatg
     3721 gcacagtcac ttggtgatct ctcccacgat gcgcggtcac ctgtgccggc ctcgctccgc
     3781 ctattgatgg cggccgaggc cttcttcgta cttgtcgccg gcgccacgct ctactgtctc
     3841 ccagcagaga ccaacaaata ttttgcttgg accattcagc cgtccctcac cgctgccatg
     3901 ctcggtgccg cctacgtcgc tgccttcgcg ctgcaggtct ccgtactccg ccaggcgcgc
     3961 atgatcgagg cgcgcgtcgg catgccggcc gtctcgttgt ttgctaccct tggtctcgca
     4021 gcgaccctcc tccatatcga ccgcttccac ttccagagtc cggagccgac ggcgcgactg
     4081 ttcgccattg tctggctgct cgtctatgtg ctcgtgccca tcgtctctgt gtggctgctc
     4141 cggccgtggt ggcgggcgct ctggcagaac gtgccgggtc gggaaccgct ggccggcgca
     4201 atgttctgga accttatgat cttgggtatg atgctctcgc tcgccggcat catcatgctg
     4261 ttgtcgccgc aaggggtcat tccgaactgg ccctggcgcc tgacgccgct gaccgcccgc
     4321 atgctcggcg cctggtccgt cggcatcggc tttgcgctca tccaggccgc gcgcctcggc
     4381 gatgaggctc aggtcaggcc cgctgccccg gctatcatcg tgctgagcgt gctgtcgctc
     4441 attgcgctcg cacgcttttc ggaccaggtg gcctggggaa cggtaacggc ggtcgtgatc
     4501 cttgtcgtcc tcatcaacct cttcgtcgtt gcggtcatcg ccgtggctcg gccgatgaac
     4561 aaccgcgctg cgcgcgggct cgcccagtga cagtgcggcc gcacgccgct gtcgctggcg
     4621 ccgggatggc cgggctcctt gctgcacgcg tgcttgccga agccggctgg agggtgtcgc
     4681 ttgtcgagcg cgaccggctc gacggcgccc cg
//
LOCUS       DAORNH010000336         4707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13543_length_4707_cov_2.578461, whole genome shotgun sequence.
ACCESSION   DAORNH010000336 DAORNH010000000
VERSION     DAORNH010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13543_length_4707_cov_2.578461"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PK264_22670"
     CDS             complement(<1..615)
                     /locus_tag="PK264_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRD78707.1"
                     /translation="MLEKTFVPADVEARIRAAWEKADAFASGRRPKAETYCIVIPPPN
                     VTGSLHMGHALNNTLQDILCRFERMRGKDVLWQPGTDHAGIATQMVVERQMMERQEPD
                     RRSIGREKFLEKVWSWKDESGGTILNQLKRLGASCDWKRERFTMDEGLSRAVLKVFVE
                     LHRKGLIYKDKRLVNWDPHFETAISDLEVENRDMKGSLWHFKYPL"
     gene            complement(672..1061)
                     /locus_tag="PK264_22675"
     CDS             complement(672..1061)
                     /locus_tag="PK264_22675"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HRD78708.1"
                     /translation="MAGFGAAPRDAVRRLLGLGPGLTPSGDDILAGMLIALHASGRAN
                     VATELAAIIASMPSELTSVISWAHLDAAAEGLPGPAIHRALIAILAGDHAALSGVLDV
                     IDTVGHCSGWDMLAGAVVALAAVAGDG"
     assembly_gap    1542..1551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1552..1922)
                     /locus_tag="PK264_22680"
     CDS             complement(<1552..1922)
                     /locus_tag="PK264_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78709.1"
                     /translation="MPAEAAIGQISDMGVIAARRLARARRGRVLAVFDRSAYVSLDLP
                     SERPGSALVCLVRGEVGPLDARTDLAWAPAGIEPEVAIEIDGRALHIGGRSVLRFADA
                     RLVEAPPWPAPRSRADLRAPLS"
     gene            complement(1929..2888)
                     /locus_tag="PK264_22685"
     CDS             complement(1929..2888)
                     /locus_tag="PK264_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRD78710.1"
                     /translation="MTARHRPQSIVVAIGGNATHPENITGTSEEQRRIAEETAHSLLP
                     LALSDCRLIITHGNGPVVGKILMRQALTRDRIPPMTLDICVAHSQGGIAYLLMQALEN
                     ALREADSARHVACLLTQVEVDPADPAFKVPTKFVGPFFAEDQAKAVGRELGWMMKQDG
                     ARGWRHVVPSPLPRHICDISLISALAERGTVVIAGGGGGIPVVRGPKGVRRGVEAVID
                     KDMTSAKIANVLGIDTLMILTAVPKVAIDFGKPTQRLLDQVSLAELERYRSEGQFPPG
                     SMGPKIDAVIAFLKGGGKRAIIASLAEAMPALDGDAGTTVLAG"
     gene            complement(2904..4382)
                     /gene="fdrA"
                     /locus_tag="PK264_22690"
     CDS             complement(2904..4382)
                     /gene="fdrA"
                     /locus_tag="PK264_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HRD78711.1"
                     /translation="MSIVVNEVLAGVYLDSVALMRISRQVAALAGVEEAGLMVGTPAN
                     REILAEAGVLAEAGHAATAGDLVIAVRAKDQATADSAIAAAKARLQETRRPAEDGGGA
                     AWQPRTIRSAIAHDPELNIALISVPGDFAIAEARKAIRRGLHAMIFSDNVPLAEEVEL
                     KREAQALGTLVMGPDCGTAIISGVPIAFANVVPRGDIGIIGASGTGIQEVSCLIAGLG
                     KGISHAIGTGGRDLKAEVGGITTLMAIDLLDADPATRHIVIISKPPAAEVAAKVLERV
                     ARSPKTFTICLLGAGDVALPPNAGLATTLTRAAEFAAGGAVAHSVSSATPRSGAVRGL
                     YSGGTLCAEAQLILTAAGFEVASNVPVPGARLVGAELHGHVVIDLGDDEFTRGRPHPM
                     IEPQVRDAPLAAALVDPNVSVILTDIVLGFGGHGDPAGHLAGMLARSDGAHPAIVASV
                     TGTDGDPQVRARQIARLEAAGVIVAPSNARAAELAARMARGT"
     gene            complement(4379..>4707)
                     /locus_tag="PK264_22695"
     CDS             complement(4379..>4707)
                     /locus_tag="PK264_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78712.1"
                     /translation="FAMAAAPAVVGFVGAGRASDAAVFTRSMAEICAGTNPGWSIPAL
                     DGAGVPTGIDVRYVVETGIAPTINTGIAHRKPGIGQVGAGIVKAPLGCFRAAVEELAA
                     KLGVAP"
BASE COUNT          769 a         1611 c         1576 g          741 t
ORIGIN      
        1 cagcggatac ttgaagtgcc agagactgcc cttcatgtcg cggttctcga cctcgagatc
       61 ggagatcgcg gtctcgaagt gcgggtccca gttgacgagg cgcttgtcct tgtagatgag
      121 gcccttccga tgcagctcga cgaacacctt gaggacggcg cgcgaaagcc cctcgtccat
      181 cgtgaagcgc tcgcgcttcc agtcgcacga ggcgccgagg cgcttcagct ggttgaggat
      241 ggtgccgccg ctctcgtctt tccagctcca caccttctcg aggaacttct cgcggccgat
      301 cgagcgccgg tcgggctcct ggcgctccat catctgccgc tcgacgacca tctgcgtcgc
      361 gatgccggcg tggtcggtgc cgggctgcca gagcacgtcc ttcccgcgca tacgctcgaa
      421 gcggcagagg atgtcctgca gcgtattgtt gagcgcatgc cccatgtgca gcgagcccgt
      481 cacgttcggc ggcgggatca cgatgcaata ggtctcagcc tttggacggc ggccggaggc
      541 gaaagcgtcg gctttctccc aggcggcgcg gatgcgcgcc tcgacgtcgg cggggacgaa
      601 tgtcttctcg agcatggcga acctgcggcg cttgtgatgc ggcccggtgg tacgatcggg
      661 cgcgggcgag gtcaaccgtc gcccgcaacc gccgcgagcg ccaccacggc cccggccagc
      721 atgtcccagc cggagcaatg gccgaccgtg tcaatcacat cgagcacgcc cgatagtgct
      781 gcatgatcgc ctgcgaggat cgcaatcaac gcgcggtgga tcgccggacc aggaaggccc
      841 tccgccgccg catcgaggtg cgcccagctg atgacgctgg tgagctcgct cggcatcgac
      901 gcgatgatcg cggcgagctc cgtcgcgaca ttggcgcggc ctgacgcatg gagcgcgatc
      961 agcatgccgg cgaggatgtc gtcgccggag ggcgttaggc ccggaccgag gccgagaagg
     1021 cggcgaaccg cgtcacgggg cgctgccccg aaccccgcca aaggcctcgg cctttggaaa
     1081 ccgacatgct cgggaatcga gaccgacgtc gattgaatgc acggagacga gtcgacatga
     1141 tccgctccac tgatgccgcc cgcgtaccct ccccccttgt gggggagggc tagggtgggc
     1201 ggtgtgcacg acgattgttg cgatgctcca cccccatcac atcgtgctcc acccccatcc
     1261 tgtagtgctc cacccccatc cccggccctt cccccgtcga gggggaaggg tgcagagctc
     1321 ccatctcgac cgggaccaac cgtacgggga cgttggggca gtgcaggtgt ggtctcggcg
     1381 gcagcggcaa tgagtttgcc ttgctccaac cagtccgcca gcacggcgat cgccgtccgg
     1441 ccgcgacggg cgagtggtgt gtccgccgat gccatgtcgg cactggcgaa cacgacgcgc
     1501 gagaggccga tggcgggtgc ccgcgcctct gcctgctcga tnnnnnnnnn ngacaatggc
     1561 gcgcgcagat ccgctcgaga acgcggcgcc ggccaaggcg gcgcctcaac gagacgcgca
     1621 tcggcgaacc gcagcaccga tcgtccgccg atgtgcaggg cgcgcccgtc gatctcaatg
     1681 gccacctcgg gctcgatgcc ggcgggggcc caagccaagt ccgttcgcgc gtcgagcggg
     1741 ccgacctcgc cgcgcacgag gcagacgagc gcggagcccg gccgctctga cggcagatcg
     1801 agcgagacat aggcgctgcg atcgaacacg gcaaggacgc gcccgcggcg cgcccttgcc
     1861 agacgtcgtg cggcgatgac gcccatgtcc gagatctgac ctatggccgc ctcagccggc
     1921 atgttggctc aaccggccag gaccgttgtc ccggcgtctc cgtccagcgc gggcatggct
     1981 tcggcgagcg aagcgatgat ggcgcgcttg ccgccgccct ttaggaaggc gatcacggca
     2041 tcgatcttcg gccccatgct accgggcggg aactgcccct cagacctgta gcgctcgagt
     2101 tcggcgaggc tcacctggtc gagcagacgc tgcgtcggct tgccgaagtc gatcgccacc
     2161 ttgggaaccg cagtcaaaat catcagcgta tcgatgccga gcacattggc gatctttgcc
     2221 gaggtcatgt ccttgtcgat aacggcctcg acgccgcggc gcactccctt aggcccgcgc
     2281 accaccggga tgccgccgcc gccgcctgcg atcacgacgg tgcctcgctc cgcaagcgcc
     2341 gagatcagcg agatatcgca gatgtggcga gggagcggcg agggcaccac gtgacgccag
     2401 ccgcgcgcac cgtcctgctt catcatccag ccgagctcgc gaccgacggc cttcgcctga
     2461 tcctcagcaa agaatggacc gacgaacttt gtcggcacct tgaaggccgg atcagccgga
     2521 tcgacctcca cctgcgtcaa caggcaggcg acatggcggg cgctgtccgc ctcgcggagc
     2581 gcattttcga gcgcctgcat caggaggtag gcaatgccgc cctggctgtg agcgacgcag
     2641 atgtcgagcg tcatcggcgg gatgcgatcg cgcgtcagcg cctgccgcat cagaatcttg
     2701 ccgacgaccg gtccgttacc gtgcgtgatg atcaggcggc aatcggaaag cgcgagcggg
     2761 aggagcgaat gcgccgtctc ctcagcgatg cgccgctgct cctcgctcgt cccggtgata
     2821 ttctctggat gcgtggcatt gccgccaatg gcgacgacga tgctttgcgg gcgatggcgc
     2881 gcggtcatgg tgggctccgg tgctcaggta cctcgcgcca tgcgtgctgc aagctcggca
     2941 gcccgcgcat tcgacggcgc aacgatcact ccggccgctt cgagacgtgc gatttgcctt
     3001 gcgcggactt gcgggtcgcc gtcggtgccc gtcaccgagg ccacgattgc cgggtgggcg
     3061 ccgtcacttc tcgcgagcat gcccgcaagg tgtccggccg gatcgccatg gccgccgaag
     3121 ccgagaacga tgtcggtgag gatcaccgat acgttcgggt cgacgagcgc ggcagcaagc
     3181 ggcgcgtcgc gcacctgagg ctcgatcatc ggatgcgggc ggccacgcgt gaactcatcg
     3241 tcgccgagat cgatgacgac atggccgtgc agttctgctc cgaccagccg cgcgccgggc
     3301 actggcacgt ttgaggcgac ctcgaaaccg gcagccgtca ggatcagctg cgcctcggca
     3361 cacagggtgc cgccagagta gaggcctcga acagcaccgg atctcggtgt tgcgctcgaa
     3421 accgaatgcg caaccgcgcc accggcggca aactcggcgg cgcgcgtcaa tgtcgtcgcc
     3481 aacccggcat tcggcggcag cgcgacatcg cccgccccga gcaggcagat cgtgaacgtc
     3541 ttcgggctcc gcgcgacccg ctcgagcacc ttggccgcga cctcggcggc cggcggcttt
     3601 gagataatca caatgtggcg cgtcgcggga tcggcatcga ggagatcgat cgccatcagc
     3661 gtggtgatgc cgccgacctc ggccttcagg tcgcgcccac ccgtgccgat ggcgtgcgag
     3721 atgcccttgc cgagccccgc aatcagacag gagacctcct ggatgccagt ccctgaggcg
     3781 ccgatgatgc cgatgtcgcc ccgcggaact acgttggcga aggcgatcgg cacgccggaa
     3841 atgatcgccg tgccgcagtc cggtcccata accagggtgc cgagggcttg cgcctcgcgt
     3901 ttcagctcga cctcctcggc gagtggcacg ttgtccgaga agatcattgc atgcaggccg
     3961 cgacggatcg ctttgcgcgc ttcggcaatg gcgaagtcac ccggcaccga gatgagcgcg
     4021 atgttgagct cgggatcgtg cgcgattgcc gagcggatcg tgcggggctg ccatgctgct
     4081 ccgccgccgt cttcggcagg tcgtcgcgtc tcctgcaacc gggccttggc cgcggcgatg
     4141 gcggaatcgg ccgtcgcctg atccttcgca cggacggcga tcacgaggtc gccggcggtc
     4201 gcggcatgac ccgcctccgc gagcacgcct gcttcggcga ggatctcgcg attggcgggc
     4261 gtgccgacca tgaggccagc ctcctcgacg ccggcgagcg ccgcgacctg gcgcgagatg
     4321 cgcatcaagg cgacggaatc gaggtagacg cccgctaaga cctcgttgac gacgatgctc
     4381 atggcgcgac cccgagcttt gccgccagct cctcgaccgc cgcccggaag cacccaagag
     4441 gcgccttgac gatgccggcc cccacctgcc cgatgccagg cttcctgtgt gcgataccgg
     4501 tgttgatcgt cggcgcgatg cctgtctcga cgacataacg gacatcgata cccgtcggca
     4561 cgcccgcccc gtcgagcgcc gggatcgacc atccgggatt cgttccggcg cagatctcgg
     4621 ccatcgagcg cgtgaagacg gcggcatcag acgcgcgtcc cgcgccgacg aaaccgacca
     4681 ctgcaggggc cgcggccatg gcgaagc
//
LOCUS       DAORNH010000337         4643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_13799_length_4643_cov_2.766347, whole genome shotgun sequence.
ACCESSION   DAORNH010000337 DAORNH010000000
VERSION     DAORNH010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_4643_cov_2.766347"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..241)
                     /locus_tag="PK264_22700"
     CDS             complement(2..241)
                     /locus_tag="PK264_22700"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRD78713.1"
                     /translation="MSKTLKLGTTVVPVHNIRTIKPITDEERARMAEAADRISTGDFD
                     VPEFSGKGKDEIAALGVAFNRMRRSLQKALKLIER"
     assembly_gap    838..915
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<916..3047)
                     /locus_tag="PK264_22705"
     CDS             complement(<916..3047)
                     /locus_tag="PK264_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD78714.1"
                     /translation="MTSVQPRLSLDGMLDLIPKSRRPLAPIFEAVSNALEAIAERRNR
                     GWSGQGEITLKLYFTGLLPETRSLERIEIIDNGIGFDDANYARFETFLDRTKGYNNRG
                     SGRVQFLHFARRIEVTSHFLAEGKPFRRHFLCNPSTYITEPSVTPIDTGVPLGTTIDF
                     RDFDLTDEAREYFDSLTIAELRNALKSHFLLRLHLARVENPEQAPVLHLQFLKNSQPD
                     GIPISLTPADIPTPETGQIDVPYVKIRDPRAEDVEWVPPPAHVETLHWAHFKLAEDEL
                     PENGVMLCSKGVQVDRVRFDGLRKAEAVEGHRFVTAIHGDVLNRAGSVSHTVDRFTLP
                     NRADTEKAIHDGALFFDPKADILFFDDIEEAVENALPSIYADLFTRKAEQQADIEAIA
                     KAHGIPPEIARATKIRLTDSEEQITEKLFRKQAETLAEQSLRIKRLFEALDQLDPSSE
                     TYQTDLEARSNELLELIPEQNKQELSRYVIRRQMVATVLGKILDGHIAPVAKPKASKS
                     SKRRLQGREALIHDLIIRRGTSTSQPHDLWVLSEEFVHFEGCSNVSIDQIKDSHGQKL
                     LRPISKADIEAYGIRKAVKPDIFLFADEGQCILIELKAPDENISEYLNQLPMYCNLIA
                     NFAVKPITRFYCYLVGETLSPIEIGGDYRQTVDGDFVRRADYAIMRYENGRQDQEIGK
                     AHIEIIKLSSLRHRALRRNKSFADRLGI"
     gene            complement(3169..3636)
                     /locus_tag="PK264_22710"
     CDS             complement(3169..3636)
                     /locus_tag="PK264_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78715.1"
                     /translation="MTATPKPTFAPLELDDIDARIEGEAARRGIPTLTLPDPADRAGQ
                     GVAIIEKAVKIDLQAPPSTRRPARTPAARRSRCEAPPGAADASDPVPTPRARMIPLNV
                     EVPDYAWVEIKSRAARDFTSMRFLILKLLRDSGIRIDDADMIEDGRRVRERRE"
     gene            complement(3633..4319)
                     /locus_tag="PK264_22715"
     CDS             complement(3633..4319)
                     /locus_tag="PK264_22715"
                     /inference="COORDINATES: protein motif:HMM:NF018691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRD78716.1"
                     /translation="MIISFASSKGGVGKSTTAAAVAAGLALAGERVQVIDLDQAQALS
                     RWSRRVTIPGLAIAAVAREDFPAYFRDLQAGDPPDHVLIDLPGVRETTVLKAIARSDL
                     VVIPSQASELDLYEALRVVSDIRAVAAETGRAVPYRLLLTKVFPLRTRVVDYAYAEMA
                     RHGLPLFRTALVERSVYKEQFLTGRPPSLAEPDKGAGREIADLIAEMQAVAAGEEGRG
                     AAGWQAEHAA"
BASE COUNT          781 a         1331 c         1557 g          896 t
ORIGIN      
        1 ctcagcgctc gatcagcttg agcgccttct gcaggctccg gcgcatgcgg ttgaacgcga
       61 cgcccagcgc ggcgatctcg tccttgccct tgccggagaa ctcgggcacg tcgaagtcgc
      121 ccgtggagat gcggtccgcg gcctcggcca tgcgggcgcg ttcttcgtcg gtgatgggct
      181 tgatggtacg gatgttgtgg accggtacga cggtggtccc gagtttcaaa gtcttactca
      241 taatacactc ctggaattga attgttgaca attccgtgga tataagtctc gtaattattc
      301 tatgtcaata aatgtttctc gcggcaatta cataaccaag gaatatgaaa ttatgtttca
      361 atttcctatt ggcgtcagaa tgccaaatgg gcgctatttt ggcgctgtga tgtcatcatg
      421 aaatcccggg cgcgcgggct gtttcgaacg gaagacctga tcccgatcgc cgaacggctg
      481 gtcgcggcgc tccaggatgc cggggtcacg catgtcgccg acgtggcggt ctactaccgg
      541 ccagcagatc aacggggagt cgacgtggca gttggaaccg acgccggacc gatcgaggaa
      601 atcacgatcg agtgctccga tctggcgttg cccatgcctg cgaacgcacc acgggttctt
      661 ccacagacac cacaagcaaa aagccggcga ggtcccctac cccggcgtca ccgaggcacc
      721 gaatagctcc cagccatccg accgccgcgc catgggatcg tcacccgcat gggcggaaac
      781 cgcgctctta cggcgattgg gatgcccgcc tcgcaaccgc gcgcgcgggt tccgtggnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnctgat cccgaggcgg tccgcgaaac tcttgtttcg acgcaaagcg
      961 cgatgacgga gggaggagag cttgatgatc tcgatatgcg ccttgccgat ctcctgatcc
     1021 tgacggccgt tctcgtagcg catgatggcg tagtcggcgc ggcggacgaa gtcgccgtcg
     1081 acggtctgtc ggtagtcgcc gccgatctcg atcggactca gcgtctcgcc gaccaggtag
     1141 cagtagaagc gggtgatcgg cttgaccgcg aagttggcga tcaggttgca gtacatggga
     1201 agctgattga ggtactcgga gatgttctca tccggtgcct tcagctcgat caggatgcac
     1261 tggccttcgt cggcaaacag gaagatgtcc ggcttcacgg ccttgcggat gccgtacgcc
     1321 tcgatgtcgg ccttgctgat cggacggagc agtttctggc cgtgactgtc cttgatctgg
     1381 tcgatgctga cgttcgagca tccttcgaaa tgcacgaact cctcgctgag tacccagaga
     1441 tcgtggggct gactggtgct ggtgccgcgc cggatgatga ggtcgtggat cagcgcctct
     1501 ctgccctgca gacgccgttt ggaactcttc gaggcctttg gtttcgccac gggagcgatg
     1561 tggccatcga ggatcttgcc gagcaccgtg gccaccatct gccggcggat gacgtagcgg
     1621 ctaagctcct gcttgttctg ctcgggaatg agctccagga gctcgttgct cctggcctcg
     1681 agatcggtct gataggtctc gctcgacgga tcgagctggt cgagagcctc gaacagccgc
     1741 ttgatgcgca ggctctgctc ggcgagcgtc tcggcctgct tgcggaataa tttctcggtg
     1801 atctgctcct cgctgtcggt cagcctgatc ttggtggcgc gggcaatctc aggcgggatg
     1861 ccatgggctt tggcgatggc ctcgatgtca gcctgctgct cggccttccg ggtgaacaga
     1921 tcagcataga tcgacggcag cgcgttctcg accgcctctt cgatgtcgtc gaagaacaga
     1981 atgtccgcct tgggatcgaa gaacagcgcg ccgtcgtgga tggctttctc ggtgtccgcc
     2041 cggttcggaa gtgtgaatcg atcgaccgta tgggagacac tgccggctcg gttcagaacg
     2101 tcaccatgga tcgcggtgac gaagcgatga ccttccacgg cctcggcttt cctgaggcca
     2161 tcgaagcgga cgcggtccac ctgcacgccc ttgctacaga gcatgacgcc attttcggga
     2221 agctcgtcct cggcgagctt gaagtgggcc cagtggaggg tctcgacatg ggccggtggc
     2281 ggcacccatt cgacatcctc cgcgcgcggg tctcggattt tcacataggg cacgtcgatc
     2341 tggcccgtct ccggtgtcgg gatgtcggca ggtgtcaggc tgatcgggat gccatccggc
     2401 tggctgttct tgaggaactg caggtgcagg acaggcgcct gctccgggtt ttcgacccgg
     2461 gcgaggtgca gtcgcaggag gaagtggctc ttcagcgcat tgcggagctc ggcgatggtc
     2521 aggctgtcga agtactccct ggcttcgtcg gtcaggtcga agtctcgaaa gtcgatggtc
     2581 gtccccagtg gcactccggt gtcgatgggc gtgaccgacg gctcggtgat gtaggtgctc
     2641 ggattgcaca gaaagtggcg tcggaaaggc ttgccctcgg cgaggaagtg gctggtcacc
     2701 tcgatgcggc gggcgaaatg gaggaactgc acgcggcctg agccgcgatt gttgtagccc
     2761 ttggtccgat ccagaaacgt ctcgaagcgg gcgtagttgg cgtcgtcgaa accgatgcca
     2821 ttgtcgatga tctcgatgcg ctccagggac cgggtctcag gcaacaaccc cgtgaagtag
     2881 agcttcaacg tgatctcgcc ctgcccgctc cagccccggt tgcggcgctc ggcgatcgcc
     2941 tccagggcat tggagacggc ctcgaagatc ggcgcgagcg gacggcgcga cttcggaatg
     3001 aggtcgagca ttccgtcgag actgaggcgc ggttggactg acgtcatagc gaaagcacag
     3061 ttcctggggt cgggtcgtcg cggagcgacc ggctgcgatt caagtaacaa ttctagccga
     3121 gaccacccga aaatgcaggc ttctggctca tgtcatcccg cgagaacatc actcccgtcg
     3181 ctcccgcacc cgccggccgt cctcgatcat gtcggcgtcg tcgatgcgga tgccgctgtc
     3241 gcgcaagagc ttgaggatca ggaagcgcat ggacgtgaag tcgcgggcgg cgcggctctt
     3301 gatctcgacc caggcgtaat cgggcacctc gacgttcaac gggatcatgc gggcgcgcgg
     3361 cgtcggcacg ggatcactgg cgtcggcggc gcctggcggc gcctcgcatc ggcttcgacg
     3421 cgcggccgga gtcctggccg gtcttcgggt ggaaggcgga gcctgcaagt cgatcttgac
     3481 cgccttttcg ataatcgcga ccccctgccc tgcccggtct gcggggtccg gcagtgtgag
     3541 cgtggggatg ccgcggcgcg cggcctcgcc ctcgatgcgg gcgtcgatgt cgtccagctc
     3601 aagcggtgcg aaggtgggtt tcggggtggc ggtcatgcgg catgctccgc ctgccacccg
     3661 gcggctcccc tgccctcttc gcctgcggcg acggcctgca tttcggcaat gaggtcggcg
     3721 atttcgcggc cggcgccctt gtcgggctcg gcgagcgacg gcggtcgtcc ggtcaggaac
     3781 tgctccttgt agacggagcg ctcgacgagg gcggtacgga acagcggcag gccgtggcgg
     3841 gccatctcgg cgtaggcgta gtcgacgaca cgggttcgga gcgggaacac tttggtgagg
     3901 agcagacggt agggcacggc acggccggtc tcggcggcga cggcgcgaat gtcggagacg
     3961 acgcggagcg cctcgtagag atcgagctcg gatgcctggc tcgggatgac gacgagatcg
     4021 gagcgggcga tggccttgag cacggtggtc tcgcgcacgc cggggaggtc gatcaggacg
     4081 tggtcgggcg gatcgccggc ctgcagatcg cggaagtagg cggggaagtc ctcgcgcgcc
     4141 acggcggcga tggcgaggcc gggaattgtg acgcggcggc tccagcggga gagggcctgg
     4201 gcctggtcga ggtcgatgac ctggacgcgc tcgccggcga gcgcgagtcc ggcggcaacg
     4261 gcggcggcgg tggttgactt gccgacaccg cctttggagg aggcgaagga aatgatcatg
     4321 acgcgatacc gggactgaaa ggcttgaaac gaatcaccga tgttcagtgt gccacatttc
     4381 tcgggctgga tgaatccctc acctgcgcaa cgcttcagat ccgcaattga gcctctgcgc
     4441 agatgcacga gcactcactc gcgcaacagc gcactcgtgc aggcgtgcat ctgcgcaaat
     4501 ccgcaaatgt ggatttgcta tgccgtgcgg cgccatctgg tggaaagcca tagtccgtcg
     4561 ggtcggcaaa ttcgaagaat ggttttccag aaaattggtc gcggggtcga cgacgttggc
     4621 gcctgagcgg ccttggagcg cgg
//
LOCUS       DAORNH010000338         4554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14134_length_4554_cov_2.665037, whole genome shotgun sequence.
ACCESSION   DAORNH010000338 DAORNH010000000
VERSION     DAORNH010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_4554_cov_2.665037"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PK264_22720"
     CDS             <1..1514
                     /locus_tag="PK264_22720"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD78717.1"
                     /translation="REALERDKVKPELLLPRLEELADWLKKTRAQLSRPSNEDAEVRR
                     LKSRAAQLLADGEFEQAMDALRSVRRQLRESRRRIEERLQDEVEGLRSHMAEEAIATG
                     RLAELALARFDYDGAADLFSEAAECLPATDRLGLWRMRLRQAEALYRKGDERGDQGAL
                     TLAIAAYNDALRIVSVAENPIEFAATTSGLGNVLCRTGEAEGAGERLAESVTAYRRAL
                     LPVVREKEPRLWALTNLQLARAQSILAERSNAAAPHREASDAYRKALEVLTRETAATD
                     WALAQLGLGNALLGLEEFEPGRQLLAEAAQAFAEALTILSRERSSSEWATAQMNLGNA
                     LLGLGELEGLSARLEDAVAAYRQALKVFTREAAPRKWALAQMNLANALASLGDRDPSA
                     TSRIEEAILSYGLALEIFTREAEPLRWAICQMNLGTALIRLGERKDKRQHWLSVAAAM
                     VPALEVFEAEGATDYADLTRRNLRKFHEQWEQLIGGVAGEAPSTRPRLSAKAR"
     assembly_gap    1624..1715
                     /estimated_length=92
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1716..2480)
                     /locus_tag="PK264_22725"
     CDS             complement(<1716..2480)
                     /locus_tag="PK264_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRD78718.1"
                     /translation="MKPVGKPAPAKVNPDVKVAAKPNSVKVVAKPAAKAPAARPVTPQ
                     KAQPIMPPPAAAARPAPVQAMPAATGAKPVVKVAIGAPPRPTPSPAQGPAAASATAAG
                     PKKPVSQRHGFKMGEFIIYPTHGIGRIVGIEEQEIAGISLELFVIHFEKDKMTLRVPT
                     AKLQAVGMRKLSEDGIVSRAMETLKGRARIKRTMWSRRAQEYEAKINSGDLVAIAEVV
                     RDLYRAENQPEQSYSERQLYEAALDRMAREIAAIERI"
     gene            2380..2592
                     /locus_tag="PK264_22730"
     CDS             2380..2592
                     /locus_tag="PK264_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78719.1"
                     /translation="MAAGFATTLTLFGLAATFTSGLTFAGAGFPTGFTAFAGGVFDLG
                     AALTTEFVDGFALAFDGLAVGFDFFT"
     gene            3390..>4193
                     /locus_tag="PK264_22735"
     CDS             3390..>4193
                     /locus_tag="PK264_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78720.1"
                     /translation="MALAHRDGTAVAKAASEALLVPKDRILHRRDQTAGELRALFAPD
                     GEVAAALRGRTQARMLLYVSGRGVREAGKPYILAGDVDVKSADDIRRRGYSLETLLDD
                     LTRVLPEATEALVLLEASFEDHGATGADAGTTRRAPSEVTLVLPSARPRIAVMLAAAA
                     DESAASDMTAGRELGLFTDTVIDGLYGLAAPGRPQVTIAELERFVTRRMSERLPALFP
                     GSSRTQRPVVISDNRAMVIARFDGPAPVRDAAGSALETRECQLLSSESDL"
     assembly_gap    4194..4215
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4281..>4554
                     /locus_tag="PK264_22740"
     CDS             4281..>4554
                     /locus_tag="PK264_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78721.1"
                     /translation="MQAADCIADEKLWNERYKSADVDRLKVFLSAVRCPAVAERVRAA
                     LSAMGVSGPASAAIAPGDEDKARREAAEKAKRDRDAAEAEAKRKAAA"
BASE COUNT          702 a         1451 c         1514 g          773 t
ORIGIN      
        1 gccgcgaggc gctcgagcgc gacaaggtca agccggaact cttgttgcca cggctcgagg
       61 agcttgccga ctggctcaag aaaacacgcg cccagctctc ccggccgtcg aacgaggatg
      121 ccgaggtgcg ccgcctcaag tcgcgcgccg cgcagctgct cgcggacggc gagttcgagc
      181 aggccatgga cgcgcttcga agcgtgcgtc ggcagctccg cgaatcgcgg cggcgcatcg
      241 aggagcggct tcaggatgag gtcgagggcc tgcgctcgca catggcggag gaggcgatcg
      301 cgaccggtcg tctcgccgag ctggcgctcg cccgtttcga ctacgacggc gccgcagatc
      361 tcttcagcga ggccgcggag tgcttgccgg ctacggatcg cctcgggctt tggcgcatgc
      421 gcctccggca ggccgaggcg ctctatcgaa agggcgacga gcgtggcgac cagggcgccc
      481 tgacactcgc cattgccgct tacaacgacg cgctgcgcat cgtctcggtc gcggaaaacc
      541 cgatcgagtt cgccgcaaca acctcgggtc tcggcaacgt gctgtgccgg actggtgagg
      601 ccgaaggtgc aggcgagcgc ctcgccgaga gcgtcacggc ctatcgccgc gctttgctgc
      661 cggtggtgcg cgagaaggag ccgcgcctgt gggcgttgac gaacctgcaa ctcgcgcggg
      721 cccaatcgat cctggccgag cgctccaatg ccgcggctcc gcaccgggaa gcgtccgacg
      781 cctatcgcaa ggcgctcgag gtgctgacac gtgagacggc cgcgacggat tgggcactgg
      841 cgcagctcgg actcggcaat gcgctgctcg gactcgagga gttcgagccg ggccggcagc
      901 tgctcgccga ggcggcgcag gcctttgcgg aggcgctcac catactctcg cgcgagcgct
      961 cgtcgtcgga atgggcaacg gctcagatga acctcggcaa cgcgttgctc ggcctcggcg
     1021 agctcgaggg gctctcggcg cggctcgagg acgcagtcgc tgcttaccgc caggcgctga
     1081 aggtgttcac gcgggaggcc gccccccgca aatgggcgct cgctcagatg aacctcgcaa
     1141 atgcgctcgc ctcgctcggc gaccgcgatc cctcggcgac ctcgcgcatc gaggaggcca
     1201 tactgagcta cggccttgcc ctcgagatct tcacgcggga ggcggagccg ctgcgttggg
     1261 cgatctgcca gatgaacctc gggacggctc tcatccgcct cggcgagcgc aaggacaagc
     1321 gccagcactg gctgtcggta gcggcggcga tggttccggc cctcgaggtg ttcgaggcgg
     1381 aaggtgccac cgactacgcc gatctcacac ggcggaacct gcgcaagttc cacgagcagt
     1441 gggagcagct catcggcggg gtagccggag aggcaccgtc gacgcggcca aggctcagcg
     1501 ccaaggcgcg ataggccgca ccggtcggca ggcaacagct ccaccgacga acgaggccgg
     1561 caatcgcgcg atcgccggcc tcgtcctcgt cagctcgccg tcgggcgggg cgttcacgcc
     1621 gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatgc gctcgatggc ggcgatctca
     1741 cgggccatcc ggtcgagagc cgcctcgtag agctgccgct cggagtagga ctgctccggc
     1801 tggttctcgg cgcgatagag gtcgcggacc acctcggcga tcgcaacgag atcgccggag
     1861 ttgatcttcg cctcgtactc ctgcgcgcgg cggctccaca tcgtgcgctt gatgcgtgcc
     1921 cggcccttga gcgtctccat cgcccgcgag acaataccgt cctcggagag cttgcgcatt
     1981 cccacggcct gcagttttgc ggtcgggact cgcagcgtca tcttgtcctt ctcgaaatgg
     2041 atgacgaaca gctcgagcga gatgccggcg atctcctgct cttcgatgcc gacgatccgg
     2101 ccgatgccgt gcgtcggata gatgatgaac tcgcccatct tgaagccgtg gcgctggctc
     2161 accggcttct tagggccggc agcggtggcg cttgcagccg caggaccctg agccggcgac
     2221 ggcgtgggcc gcgggggcgc gccgatcgct actttcacca cgggcttggc gcccgtcgcg
     2281 gccggcatag cctggacagg cgccggacga gcggcagccg ccggcggagg catgatgggc
     2341 tgagccttct gtggggtcac gggcctcgca gccggtgcct tggccgccgg cttcgcgacg
     2401 accttgacgc tgttcggctt ggccgccacc ttcacgtcgg gattgacctt cgccggcgcc
     2461 ggcttcccaa ccggcttcac agcctttgca ggcggggtct tcgatttggg agctgctttg
     2521 accacggaat ttgtcgacgg ctttgccttg gctttcgacg ggcttgcggt tggtttcgac
     2581 ttcttcactt gagccttcgt ggcggcggcc gccttcgttg ccctgttctt cttcgccgcg
     2641 cccttcccgc tggtggtgct cgcggccggc gttctcgagc cattgcgttc gtctcgctcg
     2701 ttaccaaccg tggactcgga cataactgcc gaagccacaa catcatggtg acgcaccttg
     2761 cggatgcgtt tgggcgccag aggacgcgcc gcacgtacgc gggccaatgg cagcttgccg
     2821 tgggccagcg aagtgtccat gctttgcaag acgtgtcgcg ggggcgacgt catctcgcag
     2881 cgatgatcgg tccagttgcc gacatgccgc agtcacaccc ccttacatat cgaccagacg
     2941 tgctggccgc tgagggctaa gtcgaccgca gaacatcgcg tctctcgaac ggcacctcag
     3001 ctgcgttcga gagcaacagg ctttcacact cggacgatcg tataacacaa aactcggcgc
     3061 gattgaagcg cttgtgatgc atcgatgaca tgattggcat tgaagatgct taagcgctcg
     3121 cgccggagcg acgtgtgcgg ttgcctcaat gttgcttcaa tgggtctcga atgattcgga
     3181 gactggtgct cgcatagtga acgagtctgc catagtatgg tggactggcc gatcagctat
     3241 gagagactga gatgcttggc ttccacacgc gcgctgctca gtgcttgatg ctttgcattc
     3301 tatcttttct tgcgaccggg cccgtaagcg ctggccctga cgaccttgcc gtcattgtcg
     3361 cgaaccgaag ctacacgtcc gcggccgaga tggcgcttgc gcatcgcgac ggcacggcgg
     3421 tcgccaaagc cgcgagcgag gcactgctcg tgccgaaaga ccgcattctt catcgccgcg
     3481 atcagacggc aggcgagttg cgggcgctgt tcgcgcccga cggcgaggtc gcggcagcgc
     3541 tgcgtggtcg tacacaggcg cgcatgctgc tctacgtgag cggccgtggt gtccgcgagg
     3601 ccggcaagcc ttacatcctg gcgggcgatg tcgatgtgaa gtccgctgac gacatccgcc
     3661 gacgtggcta ttcgctcgag acccttctcg atgaccttac acgtgtcctg cccgaggcga
     3721 cagaagcgct ggtgttgttg gaggcgagct tcgaggatca tggcgccacg ggcgccgatg
     3781 ccggcacgac caggcgcgcg ccctcagagg tgacgctggt gttgccgtcc gcccgaccac
     3841 ggatcgccgt catgttggcg gcggcagccg atgagagcgc ggcgtccgat atgaccgctg
     3901 ggcgagaact cgggctcttc acggatacgg tcatcgatgg cctttatgga ctggcagcac
     3961 caggccggcc tcaggtcacg atcgcagagc tcgagcggtt cgtcacgcgc cgcatgagcg
     4021 agcgattgcc tgcgcttttc ccgggatcct cgcgcacgca gcgcccggtg gtgatcagcg
     4081 acaatcgcgc catggtgatc gcgcggttcg atggtccggc gccggtcaga gacgctgccg
     4141 gctcggcgct cgagacgcgt gaatgccagc tgctttcgag cgagagcgat ctcnnnnnnn
     4201 nnnnnnnnnn nnnnngccgc atctgtgctt gcgccgatcc tctgcgcaaa cgcgtgcagt
     4261 cgctgcgcac cgacgcgagc atgcaggcgg cggactgtat tgccgatgag aagctctgga
     4321 acgagcgcta caagtctgcc gatgtcgacc gcttgaaggt cttcctctcc gccgtccgct
     4381 gccccgcggt cgccgagcgc gtgcgtgccg cactgtccgc tatgggcgtg agcggtccgg
     4441 cctcggctgc aatcgcgccg ggcgacgaag acaaggcgcg gcgcgaggcc gccgagaagg
     4501 caaagcgaga tcgcgacgcg gccgaggcgg aagccaagcg caaggcggcg gccg
//
LOCUS       DAORNH010000339         4552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14146_length_4552_cov_2.837447, whole genome shotgun sequence.
ACCESSION   DAORNH010000339 DAORNH010000000
VERSION     DAORNH010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14146_length_4552_cov_2.837447"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PK264_22745"
     CDS             complement(<1..184)
                     /locus_tag="PK264_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HRD78722.1"
                     /translation="MSAAARRLDSLAREARACRLCVEAPHGEPLPHAPRPVLRVSATA
                     RLLICGQAPGTRVHASG"
     assembly_gap    337..346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<347..972)
                     /locus_tag="PK264_22750"
     CDS             complement(<347..972)
                     /locus_tag="PK264_22750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78723.1"
                     /translation="MRSIKGEIAWVTGAGTGIGEAAALALAADGAKVILTGRRAAPLE
                     DVARRIKAQGGTAEVRAGDLSKAATVDRLAGEIRSAHGRLDILVSNAGLNIPDRSWKR
                     LTPEGIDTLVSGNLSSAIYVARACIPIMREQGGGVMIHTSSWAGRWVGAVAGPIYTAA
                     KHGVVAMSHSINLEECQNNIRSSVVCPGEVATPIMKLRDPPEPPEIMAR"
     gene            1058..>1291
                     /locus_tag="PK264_22755"
     CDS             1058..>1291
                     /locus_tag="PK264_22755"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRD78724.1"
                     /translation="MSMPRAIQRFEVSLKAFIVGADRRALFVREADTGYWELPGGRID
                     VGEEWQLHEAVLAREITEELGAGFCIEPLDRTVT"
     assembly_gap    1292..1306
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1307..1509
                     /locus_tag="PK264_22760"
     CDS             <1307..1509
                     /locus_tag="PK264_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78725.1"
                     /translation="MFVFQFIVGRMCRHAGGALALSPEHGECAWMPRAESLALGFPPS
                     SGYRDAIARLWQAAEGVGNRGA"
     gene            1636..2676
                     /gene="ruvB"
                     /locus_tag="PK264_22765"
     CDS             1636..2676
                     /gene="ruvB"
                     /locus_tag="PK264_22765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRD78726.1"
                     /translation="MTDRIVSSAKREIDAMEASIRPQSLAEFIGQEQARANLRVFIEA
                     ARARGDALDHVLFAGPPGLGKTTLAQIMARELGVGFRSTSGPVISKAGDLAAQLTNLE
                     ERDVLFIDEIHRLNPAVEEILYPAMEDYQLDLMIGEGPAARSVKIDLPKFTLVGATTR
                     AGLLTTPLRDRFGIPIRLNFYTTEELELVVTRGARVIGAPINAEGAREIARRSRGTPR
                     IAGRLLRRVRDFAAVARAPVIDAPVADKALRQLEVDNEGLDALDHRYITCIAKNYDGG
                     PVGIETIAAALSEPRDALEEIVEPYLLQQGFIGRTPRGRVLTMKAFRHIGLAGPQRQS
                     APQLGLFEEGEE"
     gene            2798..3460
                     /locus_tag="PK264_22770"
     CDS             2798..3460
                     /locus_tag="PK264_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78727.1"
                     /translation="MESPPPPLTPLTTMMVMRSKAYCLPIPAEYMTAIGAAVAQWGYY
                     EMMFSGYLVILSRHPGAHILGKLPRPFERRTARMCELARVAFPEHPSLVDKICDYSER
                     AYGISKKRNVLVHGYWFDMTPFDAQKGVQIWTKPDGTGDFYQVELSQIEALGEKIQYL
                     GIEGWSFFGNPASNQCLTPAELSTLQEFHARFPPPSHPMPPIPQDPKRTGTGQKPAPF
                     LA"
     gene            complement(3683..4150)
                     /locus_tag="PK264_22775"
     CDS             complement(3683..4150)
                     /locus_tag="PK264_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HRD78728.1"
                     /translation="MAIRFVALETELVDALRRGAPDANGQAPERHLATDDGLPCRHCL
                     ADIRKGESYLIVSHRPFPAPQPYAEQGPIFLHAAACPRGGGNGELPAFLDSPTYIVRG
                     YGANNRIVYGSGEVVATAEILAAAEKRFADPKITYVHVRSATNNCFHCRIERA"
     gene            4305..>4552
                     /locus_tag="PK264_22780"
     CDS             4305..>4552
                     /locus_tag="PK264_22780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78729.1"
                     /translation="MSDSAWASHQQPELAATAPAERAATLAWVEPEGGLTTGAGRRIL
                     LGLSTGGISNAWTRVDLRRRTWNAIRIDGTPLVHTGTPR"
BASE COUNT          758 a         1451 c         1516 g          802 t
ORIGIN      
        1 gccccgacgc gtggacgcgc gtccccggtg cctgcccgca gatgaggagc cgcgccgtcg
       61 ccgagacgcg cagcacaggg cgcggcgcat gcggcagagg ttccccgtgc ggagcctcga
      121 cgcagagccg gcaggcgcgg gcttcacgcg ccaaggaatc gagtcgccgc gccgccgcgc
      181 tcacgccttg cgcgcggcgt cgatctgctg ctggcggcca cgcatcatgc cgatatagcc
      241 gcgattgtgc gtcgggctca ccacgacctc gttcatgcac acgtgtgccg gctggcgcgc
      301 gatgaaggcg atgatctcac ccatgtcctc gggctgnnnn nnnnnncgcg ccatgatctc
      361 gggcggctcg ggcggatcgc ggagtttcat gatcggcgtc gccacctcgc ccgggcagac
      421 gacggagctg cggatattgt tctgacactc ctcgaggttg atcgaatggc tcatggcgac
      481 gacgccgtgc ttggcggcgg tgtagatcgg gcccgcgacc gcgccgaccc atcgcccggc
      541 ccacgaggag gtgtggatca tcacgccgcc gccttgctcg cgcatgatcg ggatgcaggc
      601 gcgcgcgacg tagatggcgc tcgacaggtt gcccgagacg agcgtgtcga tgccttccgg
      661 tgtgagccgc ttccagcttc gatcggggat gttgaggccg gcattgctga cgaggatgtc
      721 gaggcggcca tgggccgacc tgatctcgcc ggcgagccgg tcgacggttg ccgctttcga
      781 gagatcgccc gctcgcacct cggccgtgcc gccttgagcc ttgatgcggc gagcaacatc
      841 ctcgagaggg gccgcgcgcc gacccgtcaa tatgaccttg gcgccgtcgg ccgcgagcgc
      901 aagggcagcc gcctcgccga tgcctgttcc cgcgcccgtc acccaggcga tctcaccctt
      961 gatcgaacgc atgtgcgcct ccctgtcatt cgaggattgc caaatccggc tcgcatagtg
     1021 gtacgagacg gcggcaatgt cgaacggatc ggatcacatg agcatgccgc gcgcgattca
     1081 gcgcttcgag gtgagcctca aggccttcat cgtcggtgcc gacaggcggg cgctgttcgt
     1141 gcgcgaagct gataccggct actgggagct gccgggcggg cgcatcgacg tcggcgagga
     1201 atggcagctg cacgaggcgg ttctggcgcg cgagatcacg gaagagctcg gggccggctt
     1261 ttgcatcgag ccgctcgacc ggaccgtgac cnnnnnnnnn nnnnnntgat gttcgtcttc
     1321 cagttcatcg tcgggcgcat gtgccgtcat gcgggcggcg cgctggcact ctcgccggag
     1381 cacggcgagt gtgcctggat gccccgcgcg gagagcctgg cactcggctt tccgccgtcc
     1441 tcgggatatc gcgacgctat cgcgcggctc tggcaggcgg ccgagggtgt cggaaatcga
     1501 ggcgcgtagg aggccgcgcc gcttcgccgc gagggggcga tgcgcggcga gcaccccgcg
     1561 gctctacggg aagggtataa gtgcacgggg cgaatcagga acgcgggaga acgcgtccca
     1621 cctggaacga gctagatgac ggatcgcatc gtctcgtctg ccaagcgcga gatcgacgcc
     1681 atggaggcct cgatccgccc gcagtcgctc gccgagttca tcggccagga gcaggcgcgc
     1741 gccaacctgc gcgtcttcat cgaggcggcg cgcgcacgcg gggacgctct cgatcatgtg
     1801 ctgttcgcgg gcccgcccgg cctcggcaag acgacgcttg cccagatcat ggcgcgcgag
     1861 cttggcgtcg gcttccgctc gacatcgggc ccggtgatct cgaaggcggg cgacctcgcg
     1921 gcgcagctca ccaacctcga ggagcgcgat gtcctcttca tcgacgaaat ccaccggcta
     1981 aatccggcgg tcgaggagat cctctacccg gcgatggagg actatcagct agatctgatg
     2041 atcggcgaag ggccggcggc gcgctctgtg aagatcgacc tgccgaagtt cacgctcgtc
     2101 ggcgcgacga cgcgcgcggg actgctcact acgccgctgc gcgaccgctt cggcattccg
     2161 atccggctca atttctatac gaccgaggaa ctcgagctcg tggtcacgcg cggcgcccgc
     2221 gtgatcggcg caccgatcaa cgccgaaggg gcgcgcgaga tcgcgcggcg ctcgcgtggt
     2281 acgccgcgca ttgccggacg gctgctccgc cgtgtgcggg atttcgcggc ggtcgcacgg
     2341 gcacccgtga tcgatgcgcc ggtcgcggac aaggcgctcc gccagctcga ggtcgacaac
     2401 gaaggcctcg atgcgctcga tcaccgctac atcacctgca tcgccaagaa ctacgacggc
     2461 ggtccggtcg gcatcgagac gatcgcggcg gcgctctccg agccgcgcga cgcgctcgag
     2521 gagatcgtcg agccgtatct gctgcagcaa ggcttcatcg ggcgcacgcc gcgcggccgc
     2581 gtgctcacca tgaaggcctt ccgccatatc ggcctcgccg gcccgcagcg ccagtccgcg
     2641 cctcagctcg gcttgttcga ggagggtgag gaatgatggg aatggtgtag cgccagctgc
     2701 cgtcaccgag cgcgtcgtcc gatatatccg aaatttcgcg atgagtttgt cgtctacaac
     2761 ctgtgggtct tttggctttg attttctgga gcttctcatg gaatcacctc cgcccccatt
     2821 gactccgttg accactatga tggtcatgag atcgaaagcg tactgcctac ctatccctgc
     2881 tgaatatatg actgccattg gagccgcagt tgctcagtgg ggatactatg aaatgatgtt
     2941 cagcggttat ctcgtgatct tgtcgcgcca tccaggcgca catatcctcg gaaaattgcc
     3001 aagaccgttc gagaggagga cagcgagaat gtgcgaacta gctcgagttg ctttccccga
     3061 acatccttcg ctcgtcgaca agatctgcga ctactctgaa cgcgcatacg gcatcagtaa
     3121 gaagcggaac gttctcgtgc acggctactg gttcgacatg acacctttcg acgcccaaaa
     3181 gggcgtccag atttggacca agcccgatgg taccggcgat ttttatcaag tcgagctgag
     3241 ccagatcgaa gcgctgggtg aaaagattca atacctcggg atcgagggat ggtcattttt
     3301 tggtaatcct gcgtcgaatc aatgtttaac gccagccgaa ctttctactt tgcaggaatt
     3361 ccatgcgcgt tttccgcctc cctcccatcc gatgccacca attcctcagg atccaaagcg
     3421 gacaggaacc gggcaaaagc cggcgccatt tttggcgtaa ttcctagagc tgaatgctct
     3481 gatgcggtgt gctttggttt ctatggtctt cgaagcctgt ttcggaggct gcgtattggc
     3541 gaccagtcgc ccgcggcctt cgacgccctc ctgcccgagt tgaaggccgc aatcgccgcg
     3601 gcgatgccgg gcacggtgac gctcgtcggc gaactttcgc ggtaaccggg cagcgataag
     3661 cggagagcgg ggagcgctcc cctcacgccc tttcgatgcg gcagtggaag cagttgttgg
     3721 tcgctgacct cacgtgcacg taggtgatct tcgggtcggc gaagcgcttc tcggcggcag
     3781 cgaggatctc ggccgtcgcg accacctcgc cggagccgta gacgatgcgg ttgttggcgc
     3841 cgtagccgcg gacgatgtag gtcgggctgt cgaggaaggc cggcagctcg ccattgccgc
     3901 cgccccgcgg acaggccgcc gcatgcagga agatcgggcc ttgctcagca tagggttgcg
     3961 gcgcaggaaa gggccggtgc gagacgatga ggtagctctc gcccttgcga atgtccgcga
     4021 ggcagtgccg gcagggtaga ccgtcgtcgg tggcgaggtg gcgctcgggc gcctgaccgt
     4081 tggcgtccgg cgcgccccga cggagcgcgt cgaccaactc ggtctcgagg gcaacgaagc
     4141 ggattgccat gagtgcctcc tgaaggttgc aggaggctcg aataggccga cggagcctgc
     4201 cctgcgaccc gaatcgtgcg catgcgcgaa atcttcgccg gcgagccaag ggctcggtaa
     4261 ccgttcgtca gtaaactccc caaggtcggc tgggggagta ggcgatgtcg gacagtgcct
     4321 gggcgtcgca tcagcaaccg gagctcgccg ccactgctcc ggccgaaagg gcggcaacgc
     4381 ttgcctgggt cgagcccgaa ggcggcctga ccacgggcgc cggccggcgc atcctgcttg
     4441 ggctgtcgac cggtggcatc tccaacgcct ggacgcgtgt cgaccttcgt cgccgcacct
     4501 ggaacgcgat ccgcatcgac ggcacgccgc tcgtccatac cggaacgccg cg
//
LOCUS       DAORNH010000340         4548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14165_length_4548_cov_2.308702, whole genome shotgun sequence.
ACCESSION   DAORNH010000340 DAORNH010000000
VERSION     DAORNH010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14165_length_4548_cov_2.308702"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>359)
                     /locus_tag="PK264_22785"
     CDS             complement(<1..>359)
                     /locus_tag="PK264_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78730.1"
                     /translation="GCRECAEGCRAELAKRRDDIVGGRRQCELEGRTLDALVAQRAGI
                     AVLDAFARAAKCAAVKARAAGLVAGMLAGMVVPAPVPVAPPVPVTPAPIMPPAGPAVV
                     PPGTAPGALVRSWEGHGG"
     assembly_gap    360..403
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<404..1317)
                     /locus_tag="PK264_22790"
     CDS             complement(<404..1317)
                     /locus_tag="PK264_22790"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD78731.1"
                     /translation="MPLLLRLCAALLIAALAALPARAGPNDTAVIIANKAYDHSHEVE
                     FAHNDGERFADAARRALAVPPSRVHVHRDLTGGAMRALFGRPENPGRIAGLVNRDTKR
                     LWVYYSGHGFPDRASGKRTPYLLPRDGDPAALEYTGVALGDVRAALAALKKQMAGDAE
                     VILIVEACFSSLSSKGPVQPKVSGSAFHPDLEGDAAVIVIAAAGDDHVASWDHQAKLG
                     LFTDVLLDVLFARAGRTADGKVTLGHLKAHIERELQSRLARLYPGETRRQRPWISREA
                     PSIVLTSFSGEPPPRDADETPIAETIAV"
     assembly_gap    1694..1703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1806..>3040)
                     /locus_tag="PK264_22795"
     CDS             complement(1806..>3040)
                     /locus_tag="PK264_22795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRD78732.1"
                     /translation="RPFFGWRVVAAAFVLAAFGWGVGFYGPPIYLHAVVERTGWPVAL
                     VSGAVTAHFLVGVPVVANMPRLYRRFGIPLVTGVGAVLLAAGVVGWAIAREPWQLFAA
                     AGFSGVGWVTLGAAAVNALVAQWFVRARPAALAMAYNGASIGGVVFSPLWVALIAALG
                     FAGGASVIGLVMVAVIGALSHMVFSRTPESMGQVADGDAPGASAANVTSPRAHPLPGS
                     ALWTDGPFRTLAAGMALSLFAQIGLIAHLYSLLVPAIGKGGAGAAMGFATVCAIAGRT
                     IVGWTMPAGADRRHVAAMNLAVQVAGSVALLASGGTSVPLLLAGVALFGAGIGNVTSL
                     PPLIAQVEFVKEDAARVVPLIVAIGQATYAFAPAALGLLRSLETGVALLDHAGAPLYL
                     AAAIVQLAACACFLASRR"
     assembly_gap    3041..3065
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3177..3893
                     /locus_tag="PK264_22800"
     CDS             3177..3893
                     /locus_tag="PK264_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRD78733.1"
                     /translation="MVSPNRIAEIAAVIGEPARAAMLTVLMNGRALTASELAAVAGIT
                     PQTASTHLARLVAVELITVETQGRHRYHRLSSPSVARMLEDLMQHASASDWTRRAPRT
                     GPRDEALRRARTCYDHLAGRLGVAIADAVVAGGHVEIDDGAGLITERGRQFFTSVGIA
                     LPDPERHGRLVSRPLCRLCLDWSERRPHLAGRLGAALCRHSFEAGWVRRINGTRAVSV
                     TPKGRSQMSKLLGLELGATA"
     gene            4060..>4548
                     /locus_tag="PK264_22805"
     CDS             4060..>4548
                     /locus_tag="PK264_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRD78734.1"
                     /translation="MPRDEKRRGKKAKPVKGNMPDREQILAVLREADGKVGKREIARA
                     LGLKGGARVDLKHLLREMTEEGCLVGNRKAVKPRGALAAVTPLAITGRDDNGDLVAEP
                     IEWDESEGPRPRVIVGSARASRRAEVVAPGVGDRILARITRHGSSLDQPADTPRYEAV
                     VLK"
BASE COUNT          746 a         1528 c         1584 g          611 t
ORIGIN      
        1 cccccatgcc cctcccacga ccggaccagc gcccccggcg ctgtcccggg cgggacgacg
       61 gcgggaccgg cgggcggcat gatcggggct ggcgtgacgg ggaccggcgg cgcgaccggc
      121 acaggcgccg gcaccaccat ccccgccaac attcctgcca ccaaccccgc cgcccgcgcc
      181 ttcacggccg cgcacttcgc cgcgcgggcg aaggcgtcga gcacggctat gccggccctt
      241 tgtgcgacga gggcatcgag tgtgcggcct tcgagttcgc actggcgccg gccgccgacg
      301 atgtcgtcgc ggcgcttcgc gagctcggcg cggcacccct ccgcgcattc gcggcagccn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaacaccg cgatcgtctc
      421 ggcgatcggc gtctcatcgg cgtcgcgcgg cggcggctcg cccgagaagc tcgtgagtac
      481 gatcgacggc gcctcgcggc tgatccaggg gcgctggcgg cgcgtctcgc cggggtagag
      541 gcgggcgagg cgcgactgca actcgcgctc gatgtgcgcc ttcaggtggc cgagcgtcac
      601 cttgccgtcc gccgtacggc ccgcgcgggc aaacagcacg tcgagaagca cgtccgtgaa
      661 gaggccgagt tttgcctggt gatcccacga ggcgacatga tcgtcgcccg ccgcggcgat
      721 gacgatcacg gcggcatcgc cctcgagatc ggggtggaaa gccgagccgc tcaccttcgg
      781 ctgcacgggc cccttgctcg acaggctcga gaagcaagct tccacgatca ggatcacctc
      841 ggcatcaccc gccatctgct ttttgagagc ggcaagtgct gcgcgcacgt ctccgagggc
      901 cacgcccgta tattcgaggg cggccggatc gccgtcgcgc ggcagcaggt agggcgtgcg
      961 cttccccgag gcgcgatccg gaaagccatg gccggagtag tagacccaga gccgcttggt
     1021 atcgcggttg acgaggccgg cgatgcgtcc cggattctcc ggccgtccga acagggcgcg
     1081 catggcgccg cccgtcaggt cgcgatggac gtgcacgcgg gacggtggca cggcgagcgc
     1141 gcggcgggcg gcatcggcga agcgctcgcc gtcgttgtgc gcgaactcga cctcgtggct
     1201 gtggtcgtag gccttgttgg cgatgatgac cgccgtatcg ttcggcccag cgcgagccgg
     1261 cagtgcggcg agcgcggcga tcaggagtgc tgcgcacagg cgcaaaagca gcggcatcga
     1321 tccctcccgg cagggcaatg accgacgata cgggccgctc gcacacttgt cacgcagccg
     1381 ccgttttgcg ccggtgtatc agtcggtcgc ggcctgtgct cgccgacctc gccacggcga
     1441 tgcgatcgcg ctcgatggtc cctcaagtcg cgccgagggt cgcagcatgt ttgatctcgg
     1501 ccagaaccgc tgcgaccgtc attcgccgaa tgagagcagc gatgcccctg agctacgaca
     1561 tcgatgccgt ccgccgcgag ttcccgatca cgcggaacat gctctatgtc gattcagccc
     1621 accaggcgcc gctgtccttc agcgtgccgg ccaccctcga gcgcttcttt ggagaggggt
     1681 tagtgtggcg gttnnnnnnn nnncggaagt tcgtattcac tgtgcgggac acttgagatc
     1741 cgaacttccg aaacgccaca ctatcacgtc cgcagacaca ttggcgcagg aggaccgcgc
     1801 ggcaattacc gccgcgatgc gaggaagcag gcgcaggcgg cgagttgcac gatggcggcg
     1861 gcgaggtaga gcggcgcgcc cgcatgatcg aggagcgcga caccggtctc gagcgaccgc
     1921 agaagtccta aggctgcggg cgcgaaggcg tatgtcgcct gccctatggc gacgatcagc
     1981 ggcacgaccc gggcggcgtc ctccttcacg aactcgacct gcgcgatgag cggcggcagt
     2041 gacgtgacgt tgccgatgcc ggcgccgaac aaggcgacgc ctgcgaggag gagcggtacg
     2101 ctcgtcccgc ccgaggccag cagcgcgact gatccagcga cctgcactgc gaggttcatc
     2161 gccgcgacgt gccgtcgatc ggcaccggcc ggcatggtcc aaccgacgat cgtgcggccc
     2221 gcgatggcgc acacggtcgc aaagcccatg gcggcgcccg cgccgccttt gccgatggcc
     2281 ggtacgagca gcgaatagag gtgcgcgatg aggccgatct gggcgaagag gctcaaggcc
     2341 atgccggcgg cgagagtgcg gaacgggccg tcggtccaga gcgcggagcc gggcaatgga
     2401 tgagcccgcg gcgaggtcac gttcgcggcc gacgcccccg gggcatcacc gtcggcgacc
     2461 tgccccatgc tctcgggcgt gcgcgagaag accatgtgcg aaagcgcgcc gatgacggcg
     2521 accatgacga gcccgatgac gctcgcgccc ccggcgaagc cgagtgccgc gatgagtgcg
     2581 acccagagcg gcgagaatac gacgccaccg atgcttgcgc cgttgtaggc catggcgagc
     2641 gctgccggcc gcgcgcgcac gaaccattgc gcaacgaggg cgtttaccgc ggccgccccg
     2701 agcgtcaccc agccgacgcc ggagaacccg gccgcggcaa agagctgcca gggctcgcgc
     2761 gcgatcgccc agccgacgac cccggcggcg agcagcacgg cgccgacacc ggtgaccaag
     2821 gggatgccga accggcggta gagacgcggc atgttggcga ccaccggcac gccgacaagg
     2881 aaatgcgccg tcaccgcgcc ggagacgagt gcgaccggcc agcccgtacg ctcgacgacg
     2941 gcatgcaggt agatcggcgg cccatagaag ccgacgcccc agccgaaggc ggcgagcacg
     3001 aaggccgccg cgacgacgcg ccagccgaag aatgggcggg nnnnnnnnnn nnnnnnnnnn
     3061 nnnnngatcg atggacggga ttcgacgtgc cagacggaca gcacttaatg cttcgggcgc
     3121 ggtcgaagca ttcggcaccc gatcctgcta gcctgggtgg gtggaggtgc agcagaatgg
     3181 tcagcccgaa caggattgcc gagattgcag ccgtcatcgg cgagccggcg cgcgcggcga
     3241 tgctgaccgt gctcatgaat ggccgcgcgc tgacggcctc ggagctcgcc gccgtcgccg
     3301 gcatcacgcc gcagaccgcg agcacgcatc tcgctcggct ggtcgcggtc gagctgatca
     3361 cagtcgagac acaggggcga caccgctacc atcgcctctc gagtccgagc gtcgcgcgga
     3421 tgttggaaga cctgatgcag cacgcctcgg caagcgactg gacacgtcgt gcgccgcgga
     3481 cggggccgcg cgacgaggcg ctgcggcggg cccgcacctg ctatgatcat ctcgcaggcc
     3541 ggctcggggt cgccatcgcc gatgcggtgg tcgcgggcgg gcatgtcgaa atcgatgatg
     3601 gcgccgggct catcaccgag aggggccgcc agttcttcac gagcgtcggc atcgctttgc
     3661 cggatccgga gcggcacggt cgattggtga gccgccccct ctgccgcctc tgcctcgact
     3721 ggagcgagcg ccggccgcat ctggcgggac gtctcggcgc agcgctctgc cgtcatagct
     3781 tcgaggctgg atgggtgcgg cgcatcaacg ggacgcgagc cgtctcggtg acgccgaaag
     3841 ggcggagcca gatgtccaag ttgctcgggc tagagcttgg cgcaacggct tagcttgagc
     3901 ctttgaccgg cggaagggcg ccgaggcgcg aggccccgcc gcaaccaact tgctggctgc
     3961 ctctgccgat tgcgtgcggc ctctggcggc attccgtgat atcgagccca cttgagacag
     4021 cttcgacttg gccaaccagc ccggacaacg acacagcgca tgccacgaga tgagaagcgc
     4081 cgcggcaaga aggcgaagcc tgtcaagggc aacatgccgg atcgcgagca gatccttgcc
     4141 gtattgcgcg aggcagacgg caaggtcggt aaacgcgaga tcgcccgtgc gctcggcctt
     4201 aagggtgggg cccgcgtcga tctcaagcat cttctgcgcg agatgacgga ggaaggctgc
     4261 ctcgtcggca accggaaagc cgtcaagccg cgcggcgcgc ttgcagccgt gacgcctctc
     4321 gccatcaccg gccgcgacga caacggcgat ctcgtcgccg aaccgatcga gtgggacgag
     4381 agcgaaggcc cgcgtccgcg cgtgattgtc ggcagcgccc gcgcgagccg gcgcgccgag
     4441 gtcgtggcac ccggtgtcgg cgaccgcatt ctcgcgcgca tcacgcgaca cggctcgagc
     4501 ctggaccagc ctgccgacac gccccgctac gaggccgtcg tcctcaag
//
LOCUS       DAORNH010000341         4525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14251_length_4525_cov_2.504474, whole genome shotgun sequence.
ACCESSION   DAORNH010000341 DAORNH010000000
VERSION     DAORNH010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14251_length_4525_cov_2.504474"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>308)
                     /locus_tag="PK264_22810"
     CDS             complement(<1..>308)
                     /locus_tag="PK264_22810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78735.1"
                     /translation="MRRQFSLLAAAAMLLVAGTAPADAKCTKLAFSVNDYGKDGPIRD
                     AKSLLDKHIADHMAKMGVKKYTVGKKDVSCELFLDFGFFDEHTCKATALVCWGDAAPA
                     "
     assembly_gap    309..362
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <363..553
                     /locus_tag="PK264_22815"
     CDS             <363..553
                     /locus_tag="PK264_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78736.1"
                     /translation="GATGTAHIARGTGAARDSATPSHWLMAKMAELMAPRYGGDASLT
                     GKFPTFRPRVSICRSLAL"
     gene            700..1737
                     /locus_tag="PK264_22820"
     CDS             700..1737
                     /locus_tag="PK264_22820"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRD78737.1"
                     /translation="MVLAGPTEGPTAPATAAIASGVELALAGRDVRIVRLDDRCDRTR
                     AVEAATEAAALSPDLVIGHPCPSAAIAAAQVYAASGILFIAPATRHPALTSGRNRTAV
                     LRIAGRDDHQGRDAGRYLARLAAGRRVALLSDRTRYGLSIVADAEAMLKATGEPSPLV
                     AHLASAERDYAALLARLADSKIAVIFFAGAPQEAAILRRQMREAGLDIPFLGSDAIAD
                     PVFPQRAGDATEGTLVMQTPDPKGFPGAADVLAKLAARGETASLESLQAHAAIEGWLA
                     AIATGAPASGPRPRGAELAELIRRRMPGTILGPLAFSANGDAEFPSFAVYVWRAGNLV
                     QLDAVRQGARP"
     gene            complement(2006..2215)
                     /locus_tag="PK264_22825"
     CDS             complement(2006..2215)
                     /locus_tag="PK264_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78738.1"
                     /translation="MGYANAQARQRELNKERPAIEADEDPRIAWGRVRGEIDGLLRSG
                     RDVPAELRHKERALMVELVAQSQGR"
     gene            complement(2595..3989)
                     /locus_tag="PK264_22830"
     CDS             complement(2595..3989)
                     /locus_tag="PK264_22830"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRD78739.1"
                     /translation="MRLVAFEVEGYRSLRSLKIDCGDLTVLAGENGVGKTNIFRALEL
                     LPAAADGSICRRLAEEGGMQSVLWSGRGSERATSLRLTARFENVAYRIEVSEPLQQEA
                     ALPSEPVVREEQLYRIGPQGTTQVLRREGASIWLRDADGKRHVYTRELLPSETAISAF
                     RDSAQYPELALVRQLFLEWRFYTEFRMDPGAPARQKGLAVATAALAGDGSDLASVLAT
                     VYQLQQRPDIIGRALDDAFPGATLDVTTSDGAASYMLRLPDMARPLAAHEISEGTLQY
                     LCLIAALSSFRLPRLIALNEPEAGLHPRMLPPLARLIARASKATQVWVVTHSGGLAHE
                     IGQASGSLPRVVARRDGATVIEPVPTFRDAVRPGVQPAQPQPQPQPQPQSQPVGADPH
                     ALQAEAHRRALSDMADLLRRIKQSPEVPRALVGDFLNRLDHLHRIGAPVPPEVEKLGD
                     QLAAEMRQQPMSGR"
BASE COUNT          763 a         1508 c         1453 g          747 t
ORIGIN      
        1 tggcaggcgc ggcgtcgccc caacagacga gtgccgtcgc cttgcaggtg tgctcgtcga
       61 agaagccgaa gtcgaggaaa agctcgcagc tcacgtcctt cttgccgacc gtgtatttct
      121 tgacgcccat cttggccatg tggtcggcga tgtgcttgtc gagcagggac ttggcgtcgc
      181 ggatggggcc gtccttgccg tagtcgttca cggagaaggc aagcttcgtg catttggcgt
      241 cggcaggcgc tgtgccggca acgagcagca tcgcggcggc ggcgagcaga ctgaattggc
      301 ggcgcatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nngaggcgcc accggtacag cgcatatcgc gagaggcacc ggcgccgccc gcgattcagc
      421 gacaccctca cattggttaa tggcgaagat ggctgaacta atggcgccgc gctatggcgg
      481 ggatgcctcg ctcaccggca aatttcccac tttccggcca agggtttcga tttgccgcag
      541 ccttgcgcta tgagatgggc acggagagat ggcgtgctgc ggccgacatt gcgagaacga
      601 atggtacgag ccccctcgag cgtcctcagc ctcgcacttc tcgccgccct ggcatggtcc
      661 ggggcgagcg cccgagcgag cggagccggc gagcccttgg tggtactcgc tggccccacg
      721 gaggggccga cggcacccgc aactgcggca atcgcctcag gggtcgagct cgcgctcgcc
      781 gggcgcgatg tcaggattgt ccgcctcgac gaccgctgcg accgcacgcg cgcagtcgag
      841 gcggcaaccg aagccgcggc gctctcacct gacctcgtca tcggacaccc ctgccccagc
      901 gcggccattg cggcggcaca ggtctatgcg gcgagtggca tcctcttcat agcgccggca
      961 acacgccacc ccgccctgac gagcggccgc aaccggactg ccgttctgcg catcgccggt
     1021 cgagacgatc accagggccg cgacgccggc cgctacctcg cacggttggc ggcggggcgg
     1081 cgcgtcgcac tcctcagcga cagaacgcgc tacgggctgt cgatcgttgc cgatgccgag
     1141 gcgatgctga aggcgaccgg cgagccctcg cctctcgtcg cgcatctcgc ctcagccgag
     1201 cgcgactatg ccgcgctgct cgcgaggctc gcggattcaa agatcgccgt gatcttcttc
     1261 gcgggtgccc ctcaggaggc cgcgatcctc cgccgccaga tgcgcgaggc tggcctcgac
     1321 ataccgttcc tcggcagcga tgcgatcgcg gacccggtgt tcccgcagcg cgcgggtgac
     1381 gccactgagg gcacgctcgt gatgcagacc ccagacccga aggggtttcc cggcgcggcc
     1441 gacgtcctcg ccaagctcgc tgctcgcggc gagaccgcga gcctcgaaag cctccaggcc
     1501 cacgccgcga tcgagggatg gcttgcagcg atcgcgaccg gcgcgccggc gtccgggccg
     1561 cgtccgaggg gagcggagct cgccgagctg atccggcggc gcatgcccgg cacgatcctc
     1621 ggtccactcg ccttctcggc gaacggcgat gccgaatttc cgtcattcgc cgtctacgtc
     1681 tggcgggcag gaaatctcgt gcagctcgac gctgttcggc aaggcgcgag gccgtagcaa
     1741 gtcgcgtcgg tgctactgtc agcttgatcc ggagagggtg ggatttccta gtgtcccgat
     1801 ggcgaagttc tcgaccacga gtcgcgaact cggacgaact tcacgatcga taaggacacc
     1861 aggaatatca caagcttagt atagcgttat gttcggaaac tcgtaccgtg agagtgccac
     1921 actgtgaatg cgaacttccg aaccgccaca ctagactcgt acccggccgt gcggggccca
     1981 cgccctgttc cgatcgggtg gcccctcagc ggccctgcga ctgcgcgacg agctccacca
     2041 tcagcgcccg ctctttgtga cggagttcgg ccgggacatc acgcccgctc ctcagcaggc
     2101 cgtcgatctc gccgcgcacg cgcccccaag cgatcctcgg atcctcgtcg gcctcgatcg
     2161 ccggacgctc cttgttcagc tcgcgctggc gagcctgtgc attggcatag cccatgtgac
     2221 cctcgctcga tacttgcgac cgacaacacg cggttgcgta ctcgcggcag tccctctggc
     2281 ccagctgctg ccgggcaaga tttctgccgg aggacgctct tgcagacgtt atcgcttgag
     2341 cccccgctgg tgccgctcca gccggcagcg agcagcatgc tgcccaacgc ttagaattgt
     2401 tacgcgatgc cgtgcgcaca agtgtgacga ccgccagata tccaccgggt agtctcgcac
     2461 aagcgagaag caacgctcag gcgatacttg agcagaaccg ccgataaccg acagaataga
     2521 atgaagatct cttgaaggaa atattcgatg cgccgcggcg caaactttgt gcactgcacg
     2581 tcgagttggc ggcgtcagcg cccgctcatc ggctgctgac gcatctccgc cgccagttga
     2641 tcaccgagtt tttcgacctc gggcggtacc ggtgcgccga tccgatgcag atgatcgagc
     2701 cggttgagga aatccccgac gagggcgcgg ggcacctcgg gcgattgctt gatgcgacga
     2761 agcaggtcgg ccatgtcgct cagggctcgc ctatgcgcct ccgcctgcag cgcgtgcgga
     2821 tcggcgccga caggttgaga ttggggttgg ggttggggtt ggggttgggg ctgcgctggc
     2881 tggacaccag gtcggacggc atcgcggaag gtcgggacag gctcgatcac ggttgcaccg
     2941 tcacggcggg caacgacacg cggcagcgac ccgctcgcct gaccgatctc gtgcgcgagc
     3001 ccgccggagt gcgtcacgac ccacacctgc gtcgccttcg aggcccgcgc gatcaaccgc
     3061 gcgagcgggg gcagcatgcg gggatgaaga cccgcctccg gctcgttcaa ggcgatcagg
     3121 cgtggcaggc ggaagctgga cagggccgcg atgaggcaga gatactggag cgtgccttcc
     3181 gagatctcat gggctgcgag tggccgcgcc atgtcgggca gacgcagcat atagctcgcc
     3241 gcgccgtccg atgtcgtcac atcgagtgtc gccccaggaa aggcatcgtc gagcgcgcga
     3301 ccgatgatgt cggggcgctg ctgcagctgg tagaccgttg ccagcacgga ggcgagatca
     3361 ctgccgtcac cggcgagcgc cgcagttgcc acggcaaggc ccttctgacg ggccggcgcg
     3421 cccggatcca tcctgaactc ggtgtagaaa cgccactcga ggaagagctg ccggacgagc
     3481 gcgagctcgg gatactgagc actgtcgcgg aacgccgaga tggccgtctc ggagggcaga
     3541 agctcacgtg tgtagacatg ccgcttgcca tcggcgtctc tgagccagat cgaggcaccc
     3601 tcgcgccgga gcacctgcgt cgtcccttgc ggcccgatgc ggtagagctg ctcctcgcgg
     3661 acgacgggct ccgagggaag tgccgcttcc tgttgaagag gctcgctcac ctcgatgcgg
     3721 taggcaacgt tctcgaagcg cgcggtcagc ctgaggctcg tcgcccgttc actgccccgc
     3781 cccgaccaga gcacgctctg catcccgcct tcctcggcga ggcggcggca gatgctgccg
     3841 tcggccgcgg caggcagcag ctcgagcgcg cggaagatgt tcgtcttgcc gacgccgttc
     3901 tcgccggcga ggaccgtcag gtcgccgcaa tcgatcttga gggagcggag agacctgtac
     3961 ccttcgacct cgaacgcaac caaccgcatg tctctcccca cgacctggct gcggctcccc
     4021 tgcatcgggc tgccgctcgc ggcgtcaaac agcaatgtac catcgcctcg cctccatgca
     4081 gcgtccctca ggaacgccac ccggcaacgc aatgcgtcac gaccatgtgc atttcgaatt
     4141 ccggaagctc actgagcttt cgctccccga actcccgatc accgtcctcg tagcagcacc
     4201 gcgccgatgt caatggcgcg cggactggcc gcgttgagcg cggcagtaat tcgcgcgcct
     4261 gatcgagctg cgcgagaccc ccaaccccac agcaccacgt tggggacgag tgtggcaata
     4321 ccatggtggg gccaaagacc tatggcgact ggtaaacgcc aatcgcctga tctcgcggat
     4381 ttcctctttc catctacatt tactgcagga ttcagttgga ctgtggcgcg acgcccgaga
     4441 tctcggcgag caccgctccc gcgagataga gcgaaccgca gatcagcaca cgtaccggtc
     4501 cggcgctctg catcttgatc cgcac
//
LOCUS       DAORNH010000342         4515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14288_length_4515_cov_2.546637, whole genome shotgun sequence.
ACCESSION   DAORNH010000342 DAORNH010000000
VERSION     DAORNH010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14288_length_4515_cov_2.546637"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PK264_22835"
     CDS             <1..910
                     /locus_tag="PK264_22835"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRD78740.1"
                     /translation="SKVAELSKLNGDASEVSIEEEITRLKTKAREALASTYAKLSPWE
                     KTQVARHAERPHAMDYINGLIEEFTPLAGDRYFAEDAALLGGPGRFRGRPVMVLGQEK
                     GHDTESRIRHNFGMARPEGYRKAVRLMDMAERFRLPVLAFVDTAGAYPGVGAEERGQA
                     EAIARSTDRCLSLGVPMIACVIGEGGSGGAVAIATANRILMLEHAIYTVASPEAAASI
                     LWRDAARANDAANSMKITAQDLEDLGVIDVIVGEPVGGAHRDRAGAIARVGEAISEAL
                     AEFEALTPDKVRLQRHERFLDIGRSL"
     gene            942..1616
                     /locus_tag="PK264_22840"
     CDS             942..1616
                     /locus_tag="PK264_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HRD78741.1"
                     /translation="MSTSSIKTVELRLPASREELAKLEIGTVVYLTGRVFTAREGVYK
                     RAVEDGIALPAPPAEMGTANFHCSPAAAIEPDGRLNVGAVTATASFRFAKWLDGWFRI
                     SGCNVIIGKGGMSSEIYRKSFVPVGAIYLTTVGYGTGALLGRGIKKVVAAHWAKELGL
                     AQAMWVLDVEHFGPFIVDSDMQGNSLFERENAKIAPGLAKLYEGTRPATLRRFGETDD
                     KTDELI"
     gene            1781..>3466
                     /locus_tag="PK264_22845"
     CDS             1781..>3466
                     /locus_tag="PK264_22845"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD78742.1"
                     /translation="MLPALLILLAAVLGLSATSVRADDLAVIIANKTYVNSHEVEFAH
                     NDGERFADAARRALLIPEDRVLVFRDQGLAAMRTLVSRQVASRVTTRTKRLWVYYSGH
                     GFPLLEVGRPPEPYLLPVDGDPGVLKETAIALDDVKKQLGALKRSMAEGAEIVLVLEA
                     CFSGRSSKGAVQPKVSGSAFNVDLGLDSSILVLSATGEDHVASWDHEAKLGLFTDLLL
                     DTLFGRAGRESDGRITTAHLGAYLDRELQARLARLYPGERRLQRPWLGGRTTASVLIA
                     HGEPLPTRDPERERSETLMCTALRLSGDAQKIAEFVASCRACAEECKAALRSRRSEIE
                     RQAAYCRAEAATLDKLLKAKVARAELAGLLQALSCPEVVAKAKTALDQMPAPAPGAPG
                     GKPMGLTPSPTPSPPPSTPAPTVVPPGTAPGTLVRSWEAHGGRVTSAAVSPDGRRIVT
                     GSSDGTLKFWDAATGRELRSIDGGQSALMAVAMSPDGRWVVSSGMDATVKLWDAATWK
                     ELRAMAGHKQQVNAVAVSPDGRWVVSGSHDTTVKLWDSATGGEIRTFPGDGGWI"
     assembly_gap    3467..3486
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3631..>3850
                     /locus_tag="PK264_22850"
     CDS             3631..>3850
                     /locus_tag="PK264_22850"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78743.1"
                     /translation="MSGSSDGTAKIWDAGTGLELRTLAVPKNTTSLDVVYAVAMSPDG
                     RWVVSGRNDGKVKLWNAATGRELRTLEGH"
     assembly_gap    3851..3870
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4209..4241
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          779 a         1465 c         1486 g          712 t
ORIGIN      
        1 gagcaaggtc gccgagctca gcaagctcaa cggagatgcc tccgaggtat cgatcgagga
       61 ggagatcaca cgcctaaaga ccaaggcgcg cgaggcgctc gcctcgacct acgccaagct
      121 ctcgccctgg gaaaagacgc aggtcgcgcg ccacgccgaa cgcccgcacg ccatggacta
      181 catcaatggc ctgatcgagg agttcacgcc gctcgcgggc gaccgctatt tcgccgagga
      241 tgcagcactc ctaggcggcc cgggccgctt ccgcggacgc ccggtgatgg tgctcggcca
      301 ggagaagggc cacgacaccg agagccgcat tcggcacaat ttcggcatgg caaggccaga
      361 gggctatcgc aaggccgtga gactcatgga tatggccgag cgcttcaggc tgccggtcct
      421 cgccttcgtc gacaccgccg gcgcctatcc cggcgtcggc gccgaggagc gcggccaggc
      481 cgaggcaatc gcccgctcga ccgaccgctg cctgtcgctc ggcgtgccga tgatcgcctg
      541 cgtgattggc gagggcggct cgggcggtgc cgtcgccatt gccaccgcga accgcatcct
      601 gatgctcgag cacgcgatct acaccgtcgc ctcgcccgag gccgccgcct cgatcctctg
      661 gcgcgacgcg gcgcgcgcca acgatgccgc caacagcatg aagatcacgg cgcaggacct
      721 cgaggatctc ggcgtcatcg atgtcatcgt cggcgagccg gtcggcggcg cccatcgcga
      781 ccgcgccggc gccatcgccc gcgtcgggga ggccatctcc gaggcgctcg ccgagttcga
      841 ggccctgacc cccgacaagg ttcgactgca acgccacgag cgcttcctcg acatcggccg
      901 cagcctctga tcatgcaatc cgtcgagcgc ggaaggccct catgagcacg agctccatca
      961 agaccgtcga actgaggctg ccggcctcgc gcgaggaact cgccaagctc gagatcggca
     1021 cggtcgtcta cctgaccggt cgcgtgttca cggcgcgcga gggcgtctac aagcgcgcgg
     1081 tcgaggacgg tatcgcgctc ccggcgccgc ccgccgagat gggcaccgcg aacttccact
     1141 gctcgcccgc cgccgcgatc gagcccgatg gccgcttgaa cgtcggcgcc gtcacggcga
     1201 cagcctcgtt ccgcttcgca aagtggctcg acggctggtt caggatctcc ggctgcaacg
     1261 tcatcatcgg caaaggcggc atgtcgagcg agatctaccg gaagagcttc gttcccgtcg
     1321 gcgcgatcta tctgacgacc gtcggctacg gcaccggcgc gctgctcggc cgcggcatca
     1381 agaaggtcgt cgccgcccac tgggcgaagg agctcggcct cgcgcaggcc atgtgggtgc
     1441 tcgacgtcga gcacttcggg cccttcatcg tcgacagcga catgcagggc aactcgctct
     1501 tcgagcgcga gaacgccaag atcgcgcctg gcctcgccaa gctctacgag gggacgcgcc
     1561 ctgcaacgct ccgccgcttc ggcgagaccg acgacaaaac cgacgaactc atctgatccg
     1621 cgatcgtgtt cgggcctcac caaagctacg gcatggtgac aggcacggag gccgaatgcg
     1681 cacgacgatg ccggcgctga caaccgctca cggctgaatt gcgcggccac gcgcttcggc
     1741 tatgctcgcc ggcaattcca gattgcgccg tggagtccac atgctgccgg ccttgctcat
     1801 ccttctcgcc gccgttctcg ggctctcggc gacgtctgtc cgcgccgacg acctcgccgt
     1861 gatcatcgcc aacaagacgt acgtgaacag ccacgaggtc gagttcgcgc acaacgacgg
     1921 cgagcgcttc gccgatgcgg cgaggcgtgc gctcctcatt ccggaggacc gagtcctcgt
     1981 cttccgcgac cagggcctcg ccgccatgcg cacgctcgtc tcgcgccagg tcgcaagccg
     2041 cgtgacgacg cggaccaagc ggctctgggt ctattattcc ggccacggct ttccgctgct
     2101 cgaagttggt cggccgcccg agccttactt gctgcccgtc gacggcgacc cgggagtgct
     2161 caaggagacg gcgatcgcgc tcgacgacgt caagaagcag ttgggcgctt tgaagcgctc
     2221 catggcggaa ggcgcagaga tcgtcctcgt gctcgaagcc tgcttctccg gccgatcgag
     2281 caagggcgcc gtgcagccga aggtgagcgg cagcgccttc aacgtcgatc tcggactcga
     2341 cagctcgatc ctggtgctct cggcgaccgg cgaggaccac gtcgcctcct gggaccacga
     2401 ggcaaagctc ggcctcttca cggacctgct gctggacacg ctgttcggcc gcgcgggacg
     2461 cgagtccgat ggccggatca cgaccgcgca tcttggtgcc tatctcgatc gcgagttgca
     2521 ggcgcggctc gcgcgtctct atcccggaga gcggcgcctg cagcggccct ggctcggcgg
     2581 gcggacgacc gcgtcggtgc tcatcgccca cggcgagccg ctgccaacgc gcgatccgga
     2641 gcgcgagcgc tccgagacgc tgatgtgcac cgcgctccgt ctcagcggcg acgctcaaaa
     2701 gatcgccgag ttcgtcgcga gctgccgcgc gtgcgcggag gagtgcaagg cggcactccg
     2761 gagccgtcgc agcgagatcg agcgccaagc cgcctactgc cgcgccgaag ccgcaacgct
     2821 tgacaagctc ctgaaagcca aggtcgcccg cgccgagctc gcaggcctgc tgcaggcgct
     2881 ctcctgtccc gaggtcgtgg ccaaggcgaa aacggcgctc gatcagatgc cggcaccagc
     2941 gccaggggca ccgggtggca aacccatggg tctgacacct tcgccgacac catctcctcc
     3001 accgtccacc cctgccccca ccgtcgtccc gcccggaact gcgccgggaa cgctggtgcg
     3061 gtcgtgggag gcgcatgggg gccgcgtgac gtctgccgcg gtgtcgcctg atggccggag
     3121 gatcgtcact gggagcagtg acggtacatt gaagttctgg gacgcggcga caggccggga
     3181 actgcgcagt attgacggag gacaaagcgc gttgatggcc gtcgcaatgt cgcctgatgg
     3241 gcgatgggtg gtctcaagtg gcatggacgc tacggtgaag ctctgggacg cggcgacatg
     3301 gaaggagttg cgcgcaatgg cggggcataa gcagcaggtc aatgccgttg cggtgtcgcc
     3361 tgacggacgg tgggtggtct ctgggagcca tgacacaacg gtaaagctct gggactcagc
     3421 gactgggggg gagatacgta cttttccagg ggatggtggc tggatcnnnn nnnnnnnnnn
     3481 nnnnnnccgg atgggcagtt ggtgatctca gggggccttg gccgaacggc aaggctgtgg
     3541 gaagcggaga ccgggcggga gctgcgcacc cttacgggac atgacaacga ggtccttgcc
     3601 cttgtgctgt cgccggatgg aaggaaggta gtgtcaggta gcagcgacgg aacggcaaag
     3661 atctgggatg ctggaacggg tttggaattg cgcactcttg cagttcctaa aaatacgacg
     3721 tcattggatg tggtgtatgc tgtcgcgatg tccccggacg ggcggtgggt ggtgtcaggc
     3781 agaaatgacg ggaaagtgaa gctctggaat gcggcgacgg gccgggagtt gcgaaccctc
     3841 gaggggcatc nnnnnnnnnn nnnnnnnnnn cgcagtatct ccagacggtc ggtgggtcgt
     3901 gagcggtggt tgcgaagaca taaagtggcg gagcattgag ggcagcgatc cctgcacccg
     3961 cggcgacata aagatctggg cgatgccgta gcggttcgcc cgccccgacc gcccgaccct
     4021 cgtcatcgtc gcgaaggcag gatggacggt tgcggtgtga tgtccgtcgc ggctcgcctc
     4081 cggtcgcgaa gcgatttctc gatatcgtcg cgcgccgcga ctgcggaaat gtttggcgac
     4141 ccaacggtcg atcactatac cttggaacga gtcgctcgtg ccgacatggc tttggcgatc
     4201 cctgccttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcatagaag tggcgcctcg
     4261 aagaacggct cgcggctcga agcagaggcg ctcaggcata ccgatgccgg catctgcggt
     4321 cgagtgaacc gcccgaatca gcctcataaa atgccttgac acctgcgggt ctcgcgcgta
     4381 ccacagcgtt cgcttggcac tcgacaaagt tgagtgctaa caccccaacc tcacgaccat
     4441 gcagggccgc tcgccagcgg cgctcatcgc gcgtggacca ccacgcatga ttgtcatggc
     4501 ctattcagtc caatg
//
LOCUS       DAORNH010000343         4504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14340_length_4504_cov_5.392223, whole genome shotgun sequence.
ACCESSION   DAORNH010000343 DAORNH010000000
VERSION     DAORNH010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14340_length_4504_cov_5.392223"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1717)
                     /gene="istA"
                     /locus_tag="PK264_22855"
     CDS             complement(176..1717)
                     /gene="istA"
                     /locus_tag="PK264_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HRD78744.1"
                     /translation="MISVDTIARVRRAHFVQGKSIKAISRELRLARNTVRSIVRAEAE
                     TERRYARREQPMPQLGLYVAQLEEMLAANVKRPKRERLTYQRIFEDLRVAGYRGGYDN
                     VRRYARSWAEREGAATAEAYVPLSFAPGEAYQFDWSHECVVIGGVTTMAKVAQVRLCH
                     SRMLFVRAYPRESQEMVFDAHERAFLFFKGVAKRGIYDNMKTAVESVFIGKERAFNPR
                     FSRLMSHHLIEPTACTPAAGWEKGQVENQVGFVRKRLFSPRLKFKSYTELNAWLADRC
                     IVLAQTHPHPIEKERTVFEMFEAERTALMPYRGAFDGFHAVSASVSKTCLVRFDNNKY
                     SVASSAVGRPVDAHAYADRIVIKQDGHVVGEHERRFGRNQVAYDPWHYGPVLAKKPGA
                     LRNGAPFKDWPLPASIEKVRRKLTGSADGDRQMVKILTAVLTDGLAAVEAACAEALDA
                     GIASADVILNTIARRTQPATAAMIEPPERLALTQPPRADCGRYDALRTVLAMEASCHA
                     GGVPV"
     gene            complement(1861..1986)
                     /locus_tag="PK264_22860"
                     /pseudo
     CDS             complement(1861..1986)
                     /locus_tag="PK264_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2009..2931)
                     /locus_tag="PK264_22865"
                     /pseudo
     CDS             complement(2009..2931)
                     /locus_tag="PK264_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611875.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     assembly_gap    2932..2941
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2942..3172)
                     /locus_tag="PK264_22870"
     CDS             complement(<2942..3172)
                     /locus_tag="PK264_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRD78745.1"
                     /translation="MRKSRFTEEQIIAILREQEGGAATADVCRRHGVSSATFNKWKAK
                     FGGMDVSDAKRLKALEDENAKLKKLLAEQMPDN"
     gene            complement(3676..3790)
                     /gene="rrf"
                     /locus_tag="PK264_22875"
     rRNA            complement(3676..3790)
                     /gene="rrf"
                     /locus_tag="PK264_22875"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3931..>4504)
                     /locus_tag="PK264_22880"
     rRNA            complement(3931..>4504)
                     /locus_tag="PK264_22880"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          791 a         1496 c         1328 g          879 t
ORIGIN      
        1 cgccgcttct catcctgatt gacgcgccgt tcccatccag attgtcgcgc cataccgatc
       61 accttctcga cgccctgctg acgcttgacg ccgaccgaga cgatctcgtc gtaggccgcc
      121 cgcatgccgg cgagctgcag ctgccccatc atgtcgagga tctcagaacg ctcggtcata
      181 ctggcacgcc tccggcatga cagctcgcct ccatcgccaa cacggttctg agcgcgtcgt
      241 agcgaccgca atcggcgcgg ggtggctggg tgagcgccag tcgttccggc ggctcgatca
      301 ttgccgcagt cgccggttgc gtgcgccgcg cgatcgtatt caggatcaca tcggcgctgg
      361 cgatgccggc atcgagcgcc tcggcacatg cggcctcaac cgcggctagg ccgtcggtca
      421 gcacggcggt gaggatcttg accatctggc gatccccgtc agcgctgccg gtgagcttcc
      481 tgcgcacctt ctcgatcgat gccggcaggg gccagtcctt gaacggcgcg ccgttcctga
      541 gtgctccggg cttcttcgcc agcactggac cataatgcca gggatcgtat gcgacctgat
      601 tacgtccgaa gcgacgctcg tgctcgccga ccacgtgacc gtcctgcttg atgacgatgc
      661 gatcggcgta ggcatgcgcg tcgacgggac ggccgacagc agaggatgcg accgagtact
      721 tgttgttgtc gaagcgcacg agacaggtct tcgagaccga tgcgctcact gcgtggaagc
      781 cgtcgaaggc gccgcgatac ggcatcagag cggtgcgctc cgcctcgaac atctcgaaga
      841 cggtgcgctc cttctcgatc gggtgcggat gcgtctgcgc cagcacgata cagcgatcgg
      901 caagccaggc gttgagctcg gtgtagctct tgaacttcag tcgcggcgag aagaggcgct
      961 tcctgacgaa gccgacctgg ttctcgacct gccccttctc ccagcccgct gccggcgtgc
     1021 atgccgtagg ctcgatcagg tgatggctca tgagccgact gaagcgaggg ttgaacgctc
     1081 tctccttgcc gatgaagacg gactcgaccg ccgtcttcat gttgtcgtag atgccgcgct
     1141 tcgcgacgcc cttgaagaac aggaacgccc gctcgtgggc gtcgaacacc atctcctggc
     1201 tctcgcgcgg ataggcgcgc acgaacagca tgcggctgtg gcagagcctg acctgcgcca
     1261 ccttcgccat cgttgtcacg ccgccgatga cgacacactc gtggctccag tcgaactgat
     1321 aggcctcgcc cggcgcgaag ctcagcggca cataggcttc cgctgtcgcg gcgccttcgc
     1381 gttccgccca gctccgtgca tagcggcgga cgttgtcgta accgccccgg tagccggcga
     1441 cccgcagatc ctcgaagatc cgctgatagg tcagccgctc gcgcttcggc cgcttcacgt
     1501 tggccgccag catctcttcg agctgcgcga cgtagagccc gagctgcggc atcggctgct
     1561 cgcgccgcgc gtagcgccgc tcggtctcgg cctcggcccg cacgatcgaa cgcacggtgt
     1621 tcctcgcgag ccgcagctca cgcgagatcg ccttgatcga cttcccctga acgaaatgcg
     1681 cgcgccgtac cctcgcgatc gtatccaccg agatcatccc ccccgctccc gtgccgacgc
     1741 acgcacagga ggaaatcttc acagcgacaa gggtggggtc aaaattgcgc gccgatcacc
     1801 ccaaaacagg ggtcaaaatt gcatgccggt tcacagcttt gcggagacga tcaggcttca
     1861 catgtcggcg gcgcgcgtgc ccccgcgatc ggagccggcg aaatgccacg acttccttac
     1921 gagagctatt ccgcgcacgg accgctcggc ggggttgttc gacaggcaca tttgaccgtc
     1981 gctgacctga gccccaactt tcctccagtc agggcgagag tcctgggatg aagttttggc
     2041 tcgcgccggt cgttgcggca agagccatgt tatgcagccg gaactcttcc ggcgtgcggt
     2101 tggcaagcgc ggagtgtggc cgcactcgat tgtaatcgtc gcgccaggcg gcgatcagcg
     2161 tccgggcttc cgatagcgat gagaaggccg tttcgttcag gcactcgtcg cgcaaccggc
     2221 cgataaagct ctcggcgaag gcattttcgc tgcggcttgc ctggctggat gtaatgccag
     2281 tcgacaggcg tcgtctggct ccactttagg atggccatgc tggtgaactc cgtgccgtta
     2341 tcggaagcga atgtcttcgg tcgtccgcga cgggcgacga tggcgtccag ctcgcgcacc
     2401 acgcggctgc ctgacagcga cgtgtcggcc accagcgcca ggcattcgcg cgtgcaatcg
     2461 tcgaccactg caaggacgcg gaagcggcgg ccatcggtga aactgtcgga cacgaaatcc
     2521 agcgaccagc gctggttcgg gccggatgga agcgcgatcg gcgcgcgtgt gccaatcgcc
     2581 cgcttgcgac cgccgcgctt gcgcacctgc aagccttctt cgcggtagag ccgccggagc
     2641 cgcttcaggt tcatcgccac gccctccttc tcgagcagaa agtgcagccg acggtagccg
     2701 aagcgacgcc gcgcagcggc gagctcccga atgcggagcc gatgcgcggc gtcgctcgcg
     2761 cggagactgc ggtatctcat cgtcgatcgg tcgagaccaa gcgaccggca cgcccgccgc
     2821 tcgctcacgc catgctgcgc acagacatga gccacgactt gccgttgagc ggcgggcgtc
     2881 accactttcg cgagttcacg tccttcagga tcgcattatc gagcatcgtc tnnnnnnnnn
     2941 ngttgtccgg catttgctcg gccagcagct tcttcagctt ggcgttctcg tcttccagtg
     3001 ccttcagccg cttggcatcg gacacgtcca tgccgccgaa cttggctttc catttgttga
     3061 acgtcgccga gctgacgccg tgccgccggc acacgtccgc cgttgccgct ccgccttcct
     3121 gctcccgcaa gatcgcaatg atctgctctt cagtgaaccg tgacttccgc atcgtccgtt
     3181 tccttcctgg aggttacgga ctctacccaa atctggagga gtttgcgggg ctcaggtcat
     3241 cggcaatcgc cgcccacctc gatctcgatc gtctcggaac ccgctgacgc tgacttctcg
     3301 tcgggctcga gcgcgatcgt caccggcacc aaggccggag gcgacgccgg cttggcctca
     3361 gcgagaaagc ggcggcgcca agcgaaaacc tggttggcgt tgacgtcgta ctggcgcgcc
     3421 aagatcgaga ccgatgcccc cggcaggctc atcgccgcaa cgatctagcg cttcagcgtc
     3481 tccggacagc gcttgttccg acgccgctca tcaccacttg tgtccacatc cgacactgtg
     3541 ggcacttcct cgctcgcaaa catccatcat gtgaccatca atcggacaca aaaaagcgcc
     3601 ttcgcagcac gcgaaagcgc ctttctgtat cgattggata ttatcgatcg gaggagataa
     3661 cacacccttt ggcagacctg gcaatgacct actctcccgc gtcttgagac gaagtaccat
     3721 cggcgctggg gcttttcacg accgagttcg gaatgggatc gggtgcagca accccgccat
     3781 aaccaccagg tcagcgaaag ggtgttgaac ttctggtctg cctcgacaga ggaggcacta
     3841 acccgcccgg gccaagtctc tccccgtggg gatcgactat tggactggcg gcaatagctt
     3901 catgccacgt ttaggcattg cttaatgaga gcgttcaagc gttcgagcga ttagtatcgg
     3961 taagctgcac gcctttggat atacgcgctt ccacacccga cctatcaacg tagtggtctt
     4021 ctacggctct cgaggagaac ttgttttgag gtgggtttcc cgcttagatg ccttcagcgg
     4081 ttatcccgtc cgcacatagc taccctgctg tgccgctggc gcgacaacag gtccaccaga
     4141 ggtgcgtcca tcccggtcct ctcgtactag ggacagatcc tctcaattct ccgacaccca
     4201 cggcagatag ggaccgaact gtctcacgac gttctgaacc cagctcacgt accactttaa
     4261 atggcgaaca gccataccct tgggacctgc tccagcccca ggatgtgatg agccgacatc
     4321 gaggtgccaa acctccccgt cgatgtggac tcttggggga catcagcctg ttatccccgg
     4381 cgtacctttt atccgttgag cgatggccct tccacgcggg accaccggat cactatgacc
     4441 gtctttcgac tctgctcgac ttgtcagtct cgcagtcagg cgggcttatg ccattgcact
     4501 cgac
//
LOCUS       DAORNH010000344         4459 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14558_length_4459_cov_2.401226, whole genome shotgun sequence.
ACCESSION   DAORNH010000344 DAORNH010000000
VERSION     DAORNH010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14558_length_4459_cov_2.401226"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PK264_22885"
     CDS             complement(<1..272)
                     /locus_tag="PK264_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRD78746.1"
                     /translation="MSKNPSDPQKPHALVKIVNKRGLHARASAKFVKCAETFDAIIHV
                     TRDDQKVGGTSIMGLMMLAAGPGTEIMIEGEGPEAPEAVEALVALA"
     gene            complement(269..700)
                     /locus_tag="PK264_22890"
     CDS             complement(269..700)
                     /locus_tag="PK264_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRD78747.1"
                     /translation="MIGIVIITHAGLATEFLAAFEHVLGPQEQVATLSIGPEDDNEAR
                     RVDLIEAVKRVDRGKGVVILTDMFGGSPSNLAISVMDEVDVEVIAGVNLPMLIRLGQI
                     RKDYPLDEAIQHASSQLTQEAGRKYISVASEFLPRRKRVGS"
     assembly_gap    842..930
                     /estimated_length=89
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<931..1245)
                     /locus_tag="PK264_22895"
     CDS             complement(<931..1245)
                     /locus_tag="PK264_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HRD78748.1"
                     /translation="MADHPGDTIHATAIAVEGLAVLIRGPSGAGKSELALRCMMAGAW
                     IGERHCRARLVADDRVSLRPCGLRLLASAPAPITGRLEVRGVGLVEVEAAGETPVAAV
                     VDL"
     gene            complement(1252..3096)
                     /locus_tag="PK264_22900"
     CDS             complement(1252..3096)
                     /locus_tag="PK264_22900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HRD78749.1"
                     /translation="MAFEPSRSWWSNGVLNALSVARANGARLEVAATPFRKAWQWFSS
                     TWLHRFFTATLARRIFAANFLALIALLLSVLYVTQKNSWLIQAKSDSLLSQGEIIAAA
                     LAAGSMLDRDGISFPDRLPEIDGRGGPYRDDGFAKLELSLRPDRVQPVLRRLIGPTNT
                     RARVYTEDGTLIADTASMPVKKTDPNPEDRVRTRNAWTRIIATIWRSELPVYREIGSA
                     NGTFYKEVRDALLGTKASMVLLSDNYEQIVSIAVPINRKNSILGVLLLSTPPGEIDRI
                     IARERRVILVLAAMALAATLIASWLLRRAVAGPMSRLSSAARQVSLNINARAEIPEFA
                     DRTDEVNGLASSFREMTRALYDRIEASEQFAADVAHELKNPLAAASAMAQMLNYAKTD
                     EKRAEVVQQIQGELKRLNRLISDVSNASRLDAELARQETAPLDLRGLLDGVVTMFSDL
                     VRDSGRSVVLEAADVPLLSDGYTVDGHAGRLRQVATNLIDNAISFSPEGGTITVRLIA
                     HGRDVEFAVEDQGPGIPADKLEKVFERFYSDRPQTDGTSGRNSGLGLSISREIVRAHG
                     GRIWAENAPARKGEMPAKQADDAVSSGARFIVRLPKASVRPQTLAWRG"
     gene            complement(3266..3976)
                     /locus_tag="PK264_22905"
     CDS             complement(3266..3976)
                     /locus_tag="PK264_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD78750.1"
                     /translation="MKEKSTIALVDDERNITSALRMALEAEGYKVRTYSDGASALEAL
                     TEDPADLGIFDIKMPRMDGMELLRRMRQQSDMPVIFITSKDEEIDELFGLKMGADDYI
                     TKPFSPRLVVERVKAVLRRIAARDGVVAPTEAARVLERGKLKLDPERHTCHWDNKPVT
                     LTVTEFLILQALATRPGVVKTRDSLMDAAYDDQVYVDDRTIDSHIKRLRKKFKQVDDD
                     FDVIETLYGVGYRFKEMP"
     assembly_gap    4101..4122
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4245..>4459
                     /locus_tag="PK264_22910"
     CDS             4245..>4459
                     /locus_tag="PK264_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD78751.1"
                     /translation="MAETPAESAARATRYLMRRALKASLATLEKGSGHPYVSLVTVAT
                     EADGAPILLLSSLAVHTRNLMADSRASL"
BASE COUNT          723 a         1403 c         1461 g          761 t
ORIGIN      
        1 atcgcgagcg cgacgagcgc ctcgacggct tcaggcgctt cggggccttc gccctcgatc
       61 atgatctcgg tgccggggcc tgcggcgagc atcatcagcc ccatgatcga cgtgccgccg
      121 accttctgat cgtcgcgcgt gacatggatg atggcatcga acgtctcggc gcacttcacg
      181 aatttcgccg aggcgcgggc gtgcaggccg cgcttgttga cgattttgac cagggcgtgc
      241 ggcttctgcg ggtccgacgg attcttgctc aacttccgac cctcttgcgg cgcggcagga
      301 actcgctcgc gacgctgatg tacttgcggc ccgcctcttg ggtgagctgg ctggaggcgt
      361 gctggatggc ctcgtccagc ggatagtcct tgcggatctg gccgaggcgg atgagcattg
      421 gcaggttgac gccggcgatc acttcgacgt cgacctcgtc catcacagag atcgcgaggt
      481 tggacgggct gccgccgaac atgtctgtga ggatgacgac gcccttgccg cggtcgacgc
      541 gcttgacggc ctcgatgagg tcgacccggc gtgcctcgtt gtcgtcctcg ggcccgatcg
      601 agagcgtcgc gacctgctcc tgaggcccga gcacatgctc gaacgccgcc aggaactccg
      661 tggcgaggcc cgcatgcgtg atgatgacaa ttccgatcat cttgcaccct gtccgtctca
      721 ggccagcgga tgtttttcga agcgtccgtg atgatacaag tgatttggca ggcaacagcc
      781 actacactct gtcgcagcct ctgtgcaccc agagcagcag cttgaggtgg gcagaaggct
      841 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagatcaacg acggccgcca ccggcgtttc
      961 gcctgcggcc tcgacctcga cgaggccgac cccgcgcacc tccaggcggc ccgtgatcgg
     1021 cgccggcgcg ctggcaagca ggcgaaggcc gcagggcctc aggctgacgc gatcgtcggc
     1081 aaccaggcgc gcgcggcaat ggcgctcgcc gatccacgcg ccagccatca tgcagcgcag
     1141 tgccagctcc gacttgccgg ctcccgacgg gccgcggatg agcaccgcca gcccttcgac
     1201 ggcgatcgcc gtcgcatgga tcgtatcacc tgggtggtct gccatcgacc gtcaaccccg
     1261 ccaggcgagc gtctgcggcc ggacagaggc tttgggcagc cgcacgatga agcgggcgcc
     1321 ggaggagacc gcgtcgtcag cctgcttcgc gggcatctcg cctttgcgag ccggcgcatt
     1381 ctcggcccag atccgcccgc catgcgcacg gacgatctcg cgcgagatcg agagaccgag
     1441 accggaattt cggccggagg ttccatcggt ctgcgggcgg tccgagtaaa agcgctcgaa
     1501 caccttctcg agcttgtccg ccggtatgcc gggcccctga tcctcgacgg cgaactcgac
     1561 atcacggccg tgcgcgatca ggcgcacggt gatggtgccg ccttcgggcg agaacgagat
     1621 ggcgttgtcg atcaggttgg tcgcgacctg gcggagccgc ccggcatgac cgtcgacggt
     1681 gtagccatcg gagagcaggg ggacgtcggc cgcttcgagg acgacgctcc gcccgctgtc
     1741 gcgcacgagg tcggagaaca tggtgacgac gccatccagg agcccgcgca gatcgagcgg
     1801 tgccgtctcc tggcgcgcaa gctcggcatc gaggcgcgaa gcgttcgaga catccgagat
     1861 cagccggttg aggcgcttca attcgccctg gatctgctgc acgacctccg cgcgcttctc
     1921 gtcggtcttg gcgtagttca gcatctgcgc catggcgctc gctgcagcga gcgggttctt
     1981 gagctcgtgg gcgacatcgg cggcgaactg ctcgcttgcc tcgatgcggt cgtagagtgc
     2041 gcgcgtcatc tccctgaacg aactcgcgag cccgttcacc tcgtcggtgc ggtcggcgaa
     2101 ctctgggatc tcggcccgcg cgttgatgtt gagactgacc tggcgcgcgg ccgaggagag
     2161 ccggctcatg ggaccggcca cggcgcggcg caggagccac gaggcgatca gcgttgcggc
     2221 cagagccatc gcggcgagga ccaggatgac gcgtcgctcg cgggcgatga tccggtcgat
     2281 ctcgccggga ggcgtcgaca gcagcaaaac gccgaggatc gagttcttgc gattgatcgg
     2341 cacggcgatg gacacgatct gctcatagtt gtcgctgagc agcaccatcg acgccttggt
     2401 tccgagcagc gcgtcgcgga cctccttgta gaaggtgccg ttggcgctgc cgatctcacg
     2461 gtagacgggc agctcggagc gccagatggt tgcgatgatg cgcgtccagg cgttgcgcgt
     2521 gcgcacgcgg tcttcaggat tggggtcggt cttcttgacg ggcatcgagg ccgtgtcggc
     2581 gatcagcgtg ccatcctcgg tgtagactcg cgcccgcgta ttggtcgggc cgatcaggcg
     2641 gcgcagcacc ggctgcacgc ggtcggggcg cagggacagc tcgagcttgg cgaagccgtc
     2701 atcccgatag gggccgccac ggccgtcgat ctccggcagc ctgtcgggga atgagatgcc
     2761 gtcacgatcc agcatcgagc cggccgcgag agcagccgca atgatctcgc cctgggatag
     2821 caggctgtcg ctcttcgcct ggatcagcca ggagttcttc tgcgtgacgt agagaacgga
     2881 aaggaggagg gcaatcagtg ccaggaaatt ggcggcaaag atgcgccggg cgagcgtcgc
     2941 cgtgaagaag cgatgcagcc acgtcgagct gaaccactgc caggccttgc ggaacggcgt
     3001 cgccgcgacc tcgagccggg ctccgttcgc acgagcgacc gagagggcgt tgagcacccc
     3061 attcgaccac cacgaccggc tcggctcgaa cgccatcgac aaacgcctcc ggagatactc
     3121 caaactgggc agctccaaac ggggctgctc caatagccgg ccgcgcgccc tcccgggatg
     3181 aagtggcggg cctctcgcag gtcggccgag cacgagccaa gccacccgcg cgacactgac
     3241 gcgtcaagcg ctcaggttgc cgcctctacg gcatctcctt aaaccgatag cccactccgt
     3301 agagcgtctc aataacatcg aagtcgtcgt ccacctgttt gaacttcttg cgcaagcgct
     3361 tgatgtggct gtcgatcgtc cggtcatcga cgtagacttg gtcgtcatag gcggcgtcca
     3421 tcaggctgtc gcgcgtcttg acgacgccgg gccgcgtcgc gagcgcctgc aggatcagaa
     3481 actcggtcac tgtgagcgtg acgggcttgt tatcccagtg gcaggtgtga cgctcgggat
     3541 cgagcttcag cttgccgcgc tcgaggacgc gcgctgcctc ggtcggcgcg acgacaccgt
     3601 cgcgggccgc gatgcgccgc agcacggcct tgacccgctc gacgacgagg cgtggcgaga
     3661 acggcttcgt gatgtagtcg tcggcgccca tcttgaggcc gaacagctcg tcgatctcct
     3721 cgtccttcga ggtgatgaag atgaccggca tgtcggactg ctggcgcatg cgcctgagca
     3781 gttccatgcc atccatgcgc ggcatcttga tatcgaagat gccgaggtcg gccgggtcct
     3841 ccgtaagcgc ctcgagcgcg gaggctccgt cactgtaggt gcgcaccttg tagccctccg
     3901 cctcgagggc catgcgcagt gcggaggtga tgttccgctc gtcgtcgacc agggcgatcg
     3961 tgctcttctc tttcatagtg cggacctcat cgaagaatgg cactcttgtc accgtgtcct
     4021 atagccgttg ctgcgggtgg cccccaagtc ggacgtgctg cgacaagtac ggttcgaatt
     4081 atggccgaag gatgtgtccc nnnnnnnnnn nnnnnnnnnn nngaatgcgg ccggcgcact
     4141 gaatgcggac tgaacaagga ttctggcgat attcagatgc gtcggaggcg cgacgtggga
     4201 ggcactgtct cttgaccgat ccgcgagagc aatcacggcc cgatatggcc gaaaccccgg
     4261 cagaatcagc ggcccgggcg acacgctacc tgatgcgacg cgccctcaag gcgagccttg
     4321 ccacgctcga gaagggaagc ggccacccct atgtctcgct ggtcaccgtc gccacggagg
     4381 cggacggtgc gccgatcctg ctactttcgt cgctggccgt gcacacgcgc aatctgatgg
     4441 ccgattcgcg ggcgagcct
//
LOCUS       DAORNH010000345         4447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14605_length_4447_cov_2.473816, whole genome shotgun sequence.
ACCESSION   DAORNH010000345 DAORNH010000000
VERSION     DAORNH010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14605_length_4447_cov_2.473816"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PK264_22915"
     CDS             <1..915
                     /locus_tag="PK264_22915"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HRD78752.1"
                     /translation="KPALKAVLASAALAVATCAAVAPAAAADLPGRPGSIKDTGYAPP
                     HPHMVPAGPCYFRADVGYSWSRDPDVRWPVTNPRANPATVSGWDEDAGGNIIYYLVTD
                     QVANATMENTWFGGAGMGCGTGSRGLRGEVMFEYHGDRKVDGEPGPWFYPTPLPAPPV
                     TPPVDPLHTSVRSYTMMFNAYHDLGNFHGFVPYVGAGIGVAYHIVDDVYFTGNPALVN
                     RIHGDRDMAFAWSLMAGVGYQISQRAILDFGYRYIDMGKATSERHDSAGFLNPRVVIR
                     DMEAHEFKVGLRYHIGAAAPCCAPEPLK"
     gene            1135..1290
                     /locus_tag="PK264_22920"
     CDS             1135..1290
                     /locus_tag="PK264_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78753.1"
                     /translation="MVRILVIVCLAWLGISLAHNGGLAMLDIDDRVWWALLLPGVYLV
                     GLVLLRD"
     gene            complement(1461..1838)
                     /locus_tag="PK264_22925"
     CDS             complement(1461..1838)
                     /locus_tag="PK264_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78754.1"
                     /translation="MPVVEMQPTETLLPPSRFYFWRGASGQSYVHTIHSLIDCPAITQ
                     ACYVLVRRLGDGSMSVRAVGRTTGQSETLNLAHIRHLAASLGANEVHLHLMADGESRR
                     ARVEFDLRASLFAMLGDAAVARH"
     gene            complement(2255..>4447)
                     /locus_tag="PK264_22930"
     CDS             complement(2255..>4447)
                     /locus_tag="PK264_22930"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRD78755.1"
                     /translation="MGRIEDDLTAIFEAVKEAALTMQQGGGVGMDFSTLRPAGALVRS
                     IGADASGPVSFMDVWDAMCRTIMSAGHRRGAMMATLRCDHPDIEAFIEAKRDSARLRN
                     FNLSVLVSDAFMAAVAKGDRWALTFGGREVRSVNAPSLWDRLMRATYDVAEPGVIFID
                     RINRRNNLAYCERIAATNPCGEQPLPPYGACLLGSINLARLVTKPFEREARIDLARLE
                     RLTRDAVRFLDNIIDVSGFPLEPQRREALAKRRLGLGVTGLADALAMCGLGYGDAAAA
                     RLAAGWMAAIQGAAYEASAELAGEKGAFPLFEAAPFLAAPAVAALPESVRQSIRRHGI
                     RNGCLTSIAPTGTISLLAGNVSSGIEPIFDLAYTRRVRQADGAQRETNVEDWAVAEWK
                     RLRGPQATLPPAFVTAGSVNPRGHLEMQAALQPHVDGAISKTINCPVDMPFESFRDIY
                     SQAYELGLKGCTTFRPNAITGSVLETLPQPVVAPAPPVAAIVIGSSSAPVIPPTPEQV
                     PGVGGSAGRTPGDMADIHYMSRPLARDDVLPGFTYKLRWPGSDHAIYVTINDIIRDGR
                     RRPFEIFINTKNLEHYAWMVALTRMISAVFRRGGDVSFIAEELGAIFDPQGGRWMDGH
                     YVPSLQAAIGNVIDDHMRRIGALSETEHKIADDARAPQSRLIARGDATDPAADGVRAG
                     PARSFGSAGPGEGDTSALAGSCRRCGANAVVRDGRCWVCRNCGYARCG"
BASE COUNT          733 a         1483 c         1486 g          745 t
ORIGIN      
        1 aaacccgcct tgaaggccgt tctcgcctcg gccgcactcg ccgttgcgac ctgcgccgcc
       61 gtcgccccgg ccgccgccgc cgatctgccc ggccgcccgg gctcgatcaa ggacacgggc
      121 tatgcgccgc cgcatccgca catggtgccg gccggcccct gctacttccg cgccgacgtc
      181 ggatattcct ggtcgcgcga cccggatgtg cgctggcctg tgaccaaccc gcgcgccaat
      241 cccgccactg tgtcgggttg ggatgaggat gctggcggca acatcatcta ctatctcgtg
      301 accgaccagg tcgccaatgc caccatggag aacacctggt tcggcggcgc gggcatgggc
      361 tgcggcacgg gctcgcgggg gctccgcggc gaagtcatgt tcgagtatca cggcgaccgc
      421 aaagtcgacg gcgagccggg cccctggttc tacccgacgc cgctgccggc tcccccggta
      481 actccgcccg tcgacccgct gcacacgtcc gtgcgctcct acaccatgat gttcaacgcc
      541 taccacgatc tcggcaactt ccacggtttc gtgccgtatg tcggtgccgg catcggcgtc
      601 gcctaccaca tcgtcgacga cgtctacttc acggggaacc ccgcgctcgt caatcgcatc
      661 cacggcgatc gcgacatggc ctttgcctgg tcgctgatgg ccggtgtcgg ctaccagatc
      721 agccagcgtg cgatcctcga cttcggctat cgctacatcg acatgggcaa ggcgacctcc
      781 gagcgtcacg actcggcggg cttcttgaac ccccgcgttg tgatccgcga tatggaagcg
      841 cacgagttca aggtcggcct gcgctaccac atcggtgcgg ctgccccctg ctgcgcgccg
      901 gaaccgctga agtaagcatc gtggaaagtg ggtcggcgtc cgccggcctg agacgggagg
      961 ccgggccatg tgcccggctt tctgcgtggc tgcgccactc cgcccgagac ggcggcgagt
     1021 gcgccgccgg cccttgccgc ggcctcttcc tgccaaggcg aggcgcctcg acccggtcga
     1081 atggcatgac acttgctcga tcgccgtcat cggctcatcg ggttggagcg gcgcatggtg
     1141 cgcatcctcg tcatcgtctg cctcgcttgg ctcgggattt cgctcgcgca caatggcggc
     1201 ttggccatgc tcgacatcga cgatagggtc tggtgggcgc tgctgctgcc gggcgtctat
     1261 ctcgtcgggc tcgtcctact gcgtgactga cgggccggat ggctcagcaa tgcatcgccg
     1321 agctgactcg acggttcaat gccgtgaggc ttgcagccga cagcaacccg accgcccaat
     1381 tgcggcaacg caagattgtg acgtgccgac cccgaatgcc ctgggccgat gccgattcga
     1441 atagggcgcg ggtggccatc tcagtgccgg gcgactgcgg catcgcccag cattgcgaac
     1501 aggctggccc gcagatcgaa ttcgacccga gcgcgccgcg actcgccatc ggccatcagg
     1561 tggaggtgca cttcgttggc gccgagggac gcggcgagat ggcggatgtg tgcgaggttc
     1621 agcgtctctg actgccccgt cgtgcgcccg acggcccgta cgctcatcga gccatcgccg
     1681 aggcggcgca cgagcacgta gcacgcctgg gtaatcgcgg ggcagtcgat gaggctgtgg
     1741 atggtgtgca cgtaggactg gcctgaggcg ccgcgccaga agtagaagcg cgagggtgga
     1801 agaagcgtct cggtcggctg catctcgacg actggcatcg cggttccggc gccaacccaa
     1861 gctgacgcgg cgaacttacg atccgatgcg agcctcggct ccgggggcaa gctgcgatcc
     1921 agggggaagc tgcgggcagc catctcacgc tccgaatatc gacgtccaaa ggacaggcaa
     1981 cagggcccgg tcgcccgggg caacaactcg aatttccggc agttcagccg gttcaacgcg
     2041 ccgcaccatc agccggtttc gggtcagccg ttttcgagcc ggccggttcc atcgacgttt
     2101 ggttcaaggg agcatactgt cccgaggcaa cctgcagact catacgtgtg cagcgtaggc
     2161 taggtttgga gctgaattgt gaccgcatgc gacaaattgc ctggatctcg atgccaccag
     2221 tggtgctgcc ggtgctgaca gtccgggccg caggtcagcc gcagcgcgca tagccacagt
     2281 tgcggcacac ccagcatcgg ccgtctcgaa cgacggcatt ggctccgcaa cgccggcacg
     2341 agcccgccag agcactcgta tcgccttccc ctggtcctgc gctgccgaac gatcgtgccg
     2401 gcccggcgcg cacgccgtcg gcagccggat cggtggcatc tccacgcgcg atgagtcgcg
     2461 actgcggagc gcgggcgtcg tcggcgatct tgtgctccgt ttctgacaaa gctccgatgc
     2521 gacgcatgtg gtcatcgatc acattgccga tggcggcctg caggctcggc acgtagtggc
     2581 cgtccatcca gcgcccgcct tgcggatcga aaatggcgcc aagctcctcg gcaatgaacg
     2641 agacatctcc accacgccgg aacacggcgc tgatcatgcg cgtcagcgca accatccagg
     2701 cgtaatgctc gaggttcttc gtattgatga agatctcgaa cggccggcga cgcccatcgc
     2761 ggatgatgtc gttgatggta acgtagatgg cgtggtcgct gcccggccag cgcaacttgt
     2821 acgtgaaccc cggcagcaca tcgtcccggg caagtggccg tgacatgtag tggatgtcgg
     2881 ccatgtcacc gggggttctc cctgcactgc cgccgacacc tggcacctgc tcgggcgtcg
     2941 ggggaatgac gggcgctgag ctgcttccga tcacgattgc agccaccggc ggggctggcg
     3001 ccactaccgg ttgcggcaag gtctcaagca cggagcccgt aatggcgtta ggccggaatg
     3061 tggtgcagcc cttgaggccg agctcgtagg cttgcgaata gatgtcgcgg aacgactcga
     3121 acggcatgtc gaccgggcag ttaatggtct tggaaatggc gccgtcgaca tggggctgca
     3181 aggcggcctg catctcgaga tggccgcgcg ggttgacgct gcccgccgta acgaaggcag
     3241 gcgggagggt cgcctgcggc cctctaaggc gcttccattc cgcgaccgcc cagtcctcga
     3301 cgttggtctc gcgctgggct ccatccgcct ggcggacgcg gcgcgtgtag gcgaggtcga
     3361 aaataggctc gatgccgctc gacacattgc cggcgaggag ggagatggtg cccgtcggcg
     3421 cgatcgacgt caggcagccg ttgcggatgc catggcggcg gatcgactgg cggacgcttt
     3481 cgggcagggc cgcgacggcg ggagcggcga ggaacggagc tgcctcgaag agggggaatg
     3541 cacccttctc acccgcaagc tccgcactcg cctcgtaggc agcgccctgg atggcggcca
     3601 tccagccggc ggccaggcgc gcggcggccg cgtcaccgta tccgagcccg cacatggcga
     3661 gcgcatcggc aagtcccgtc accccgagac ccaaccggcg cttcgccaag gcctcgcgcc
     3721 gttgcggctc gagcgggaag cccgagacgt cgatgatgtt gtcgaggaag cgaacggcat
     3781 cccgggtgag gcgctcgagc cgggcgagat cgatcctcgc ctcacgctcg aacggtttcg
     3841 tgaccaggcg ggcgaggttg atcgagccga gcagacaggc gccatagggc ggcaacggct
     3901 gctcgccgca gggattggtg gcagcgatcc tctcgcagta ggcgaggttg ttgcgcctgt
     3961 tgatgcggtc gatgaagatc acgcccggct cggcgacgtc gtacgtcgca cgcatcagac
     4021 ggtcccagag cgacggggca ttcaccgagc gcacctcacg tccgccgaac gtgagggccc
     4081 agcgatcacc cttggcgacg gccgccatga aggcatcgct caccagtacg gagagattga
     4141 aattgcgcag gcgggcggaa tcgcgcttcg cctctatgaa ggcctcgata tccgggtgat
     4201 cgcagcgcag cgtcgccatc atggcgccgc gccgatgtcc ggccgacatg atggtgcggc
     4261 acatggcgtc ccagacgtcc atgaagctca ccggtcccga cgcgtcggca ccgatgctgc
     4321 gcacgagcgc gcctgccggc cggagcgtcg agaagtccat gccgaccccg ccgccctgct
     4381 gcatcgtgag cgcggcttcc ttgacggcct cgaagatggc agtgagatcg tcctcgatcc
     4441 gccccat
//
LOCUS       DAORNH010000346         4420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14739_length_4420_cov_2.631157, whole genome shotgun sequence.
ACCESSION   DAORNH010000346 DAORNH010000000
VERSION     DAORNH010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14739_length_4420_cov_2.631157"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1518
                     /locus_tag="PK264_22935"
     CDS             157..1518
                     /locus_tag="PK264_22935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD78756.1"
                     /translation="MMRQMSLVGFLQAQNCTNFSASWRHPDSRMDFTTPEYYQHIAKV
                     LEAGKFDCGFFDDRLSMPEFQSGSYEEAVEGGVRCVKIDPIACLMSMAAVTSKLGLGA
                     TYSTTYYEPFHVARVFQTLDHMTKGRVGWNIVTSLNDAEAYNMGRDEHLSHDVRYDRA
                     DEFLEIVTGHWDSWDDDPYILDKAGNRFADPKKVRRLDYKGKYLSSRGPFTVPRSPQG
                     HPVLIQAGQSGRGRKFAAEWGEMIFTHLPTRAQARASYAELKADCAKAGRNPDHMKIC
                     SLVYPIVAATKAEAEDRCAAIAKLPREVDSLLLLSEALNVDFGAKGMDEPFSDAEIAK
                     MSLQAIRDRVLKASGKKNPTPREFIKYSGRGQLHNPWVGGPKEVADMFEEWFSMPACD
                     GFVIAATYVPGNYEDFVKFVVPELQRRGLFRTEYPGTTLRDTVGLPRPKVGHWKQVTR
                     LPS"
     gene            complement(1591..2118)
                     /locus_tag="PK264_22940"
     CDS             complement(1591..2118)
                     /locus_tag="PK264_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78757.1"
                     /translation="MMVETVVKYAPICWFMPIGLMVLYSIWMPWSGSRIARAGLVLMA
                     VATVAALAAVLIGAWLGDAEKIFMALRNTVIVPVLALIAAAALVGLGDVARQLLAARS
                     LSHDAAARRDITTRLVTFAGMVALMIVISLGIGEGAGGVTLTGIKSSNWLTGLVILLA
                     VAGTLGQRPARETNR"
     gene            2564..3007
                     /gene="rpoZ"
                     /locus_tag="PK264_22945"
     CDS             2564..3007
                     /gene="rpoZ"
                     /locus_tag="PK264_22945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRD78758.1"
                     /translation="MARVTVEDCVDKVQNRFELVLIAGHRARAIANGSAITVEPEKDK
                     NPVIALREIAEETVNADDMREQLISSIQKNTEIDEPEAGAAPIVATDRRGQLIGRDDQ
                     SADMAVDHITISEEQLLRGMESMTPMEPSASGNGGGSGGSGSRGR"
     gene            3150..>4072
                     /locus_tag="PK264_22950"
     CDS             3150..>4072
                     /locus_tag="PK264_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRD78759.1"
                     /translation="MMRQFDLVERVQRYDPTADTDLLNRAYVYAMRKHGNQKRKSGEL
                     YFSHPLEVAAILTDLRLDDATIATALLHDVIEDTATTRDEIDSLFGSEIGALVEGLTK
                     IKRLDLVTQKAEQAENFRKLLVAISTDIRVLLVKLADRLHNMRTLEHNSPESRKRNAE
                     ETLEIYAPLAGRMGMQAIREELEDLSFRWLNPEAYATVTEKLGQLRERNQGLVDEIAG
                     ALREKLAGLGIRAEVSGREKKPYAIWRKMVNKQISLEQLSDIYAFRVSVEQRQECYGV
                     LGVIHSAWRAVPGRFKDYISTPKQNGYQSIHT"
     assembly_gap    4073..4157
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4158..>4420
                     /locus_tag="PK264_22955"
     CDS             <4158..>4420
                     /locus_tag="PK264_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRD78760.1"
                     /translation="EGGKAAIERAPGESDGRDGPYMWLRSLVDTLLKGDNPEEFLEHT
                     KLELFQDQVFCFTPKGRIIALPRGATPIDFAYAVHTDVGNSCVG"
BASE COUNT          816 a         1402 c         1405 g          712 t
ORIGIN      
        1 aggcagtagg cagtaggcag taggcagtag gcagtaggca gtaggcagta ggcaggattc
       61 cggtgtgtcg gcgcaaccct gctgcgctgc aaaatctatt gcctattggt cattgcctat
      121 tgccatctca caccgtgccc ccaatccgcg gacccgatca tgcgccagat gtctctcgtc
      181 ggctttctgc aagcccagaa ctgcacgaac ttctccgcct cctggcgcca ccccgacagc
      241 cggatggatt tcacgacacc cgagtactac cagcacatcg ccaaggtgct cgaagcgggc
      301 aagttcgact gcggcttctt cgatgatcgc ctgtcgatgc cggagttcca gagcgggagc
      361 tacgaggagg ccgtagaggg cggcgtccgc tgcgtgaaga tcgacccgat cgcctgcctg
      421 atgagcatgg cggccgtcac gtcgaagctg ggcctcggcg ccacctactc gacgacctac
      481 tacgagccct tccatgtcgc gcgcgttttc cagacgctcg atcacatgac caaggggcgc
      541 gtggggtgga acatcgtcac ttcgctcaac gatgccgagg cctacaacat gggccgcgac
      601 gagcatctct cgcacgatgt ccgctacgac cgggcggacg agttcctcga gatcgtcacg
      661 ggccattggg acagctggga cgacgatccc tacatcctcg acaaggccgg caaccgtttc
      721 gccgacccta agaaggtgcg acgcctcgac tacaagggaa agtatctctc ctcgcgtggg
      781 ccgttcaccg tgccgcgctc gccgcaaggc catcccgtcc tcattcaagc aggccagagc
      841 ggtcgcggtc gcaagttcgc cgccgagtgg ggcgagatga tcttcacaca cctgccgacg
      901 cgcgcgcagg cacgcgcgtc ctatgcggag ctgaaggccg attgcgccaa ggccggccgc
      961 aatcccgacc acatgaagat ctgctcgctc gtctacccga tcgtcgccgc caccaaggcc
     1021 gaggccgagg acaggtgcgc agcaatcgcc aagctgccac gcgaggtgga cagcctcctc
     1081 ctcctctccg aggcactcaa cgtcgacttc ggcgccaagg gcatggatga gccattcagc
     1141 gatgccgaga tcgccaagat gagcctgcag gcgatccgcg atcgcgtgct caaggcctcc
     1201 ggcaagaaga acccgacgcc gcgcgagttc atcaagtact cgggccgcgg acagctgcac
     1261 aatccctggg tcggcgggcc gaaggaggtc gccgacatgt tcgaggagtg gttctcgatg
     1321 ccggcctgtg acggcttcgt catcgcggcg acgtacgtcc ccgggaacta cgaggacttc
     1381 gtgaagttcg tcgttcccga actgcagcgg cgcggcctct tccgcacgga gtatcccggc
     1441 acgaccttgc gcgacaccgt ggggctgccg cgtcccaagg tcgggcactg gaagcaagtg
     1501 acccgcttgc cgtcctgatg cgcacggctg ccggtgcgag gtcgcatcgg cagccgcccg
     1561 aaaagcgagg caggcgcggg cgaatgtccg ctatcgattg gtctcccggg caggacgctg
     1621 gccgagggtg ccggccaccg caagcaggat cacaagcccg gtgagccagt tgctgctctt
     1681 gatgccggtg agcgtcacgc cgccggcgcc ttcgccgatg ccgaggctga tcacgatcat
     1741 cagcgccacc atgccggcga acgtcacgag gcgcgtggtg atgtcccgtc gagcggccgc
     1801 gtcatggctg agcgagcgcg cggccaggag ctggcgggcg acgtcgccga ggccgactag
     1861 ggctgcagcc gcaatcagcg ccagcaccgg cacgatgact gtattgcgaa gtgccatgaa
     1921 gattttctcg gcatcgccga gccatgcgcc gataagcacg gcggcgaggg ccgcaacggt
     1981 ggcaacggcc atgagaacga ggccggcgcg ggcgatgcgc gacccgctcc agggcatcca
     2041 gatgctgtag aggaccatca ggccgatagg catgaaccag cagatcggcg cgtacttgac
     2101 cactgtctcg accatcatcg ctattctccg tcgctaaccc ctccgatccg aacgggctgg
     2161 accggctctg ctccagtctt gggatgaaca gacgttaatc tgtgtcgatc cgccgccgat
     2221 acggtctggc gcccgcagtt attcattcgg cacgtgacct cgggccggtt ggcctgggcc
     2281 tgatctcatc gacgcgattg cgctggatct tagatcgtgc catatggagg cagtcgccct
     2341 gggtgtagcc gcacctgggg tttagtgtgt ctgatgtgtg gcagttcgag cttctctgcg
     2401 ggcggcccgt tcgtggccat gcagggcgca agcatgagac gggccggtga ggtcgctggc
     2461 cggatgcggg cgatggcagt ttgctcgcgc aggccttggc tttgcgtcat ggccccgtga
     2521 tgtttccgga ctgacacctc gggatcgtag aagagggatc gacatggcgc gcgtgaccgt
     2581 cgaagattgc gtcgacaagg ttcagaaccg gttcgagctc gttctgatcg cgggccatcg
     2641 cgcccgagcg atcgccaacg gctcggcgat cacagtcgag cctgagaagg acaagaaccc
     2701 ggtgattgca ctccgcgaga tcgccgaaga gaccgtcaat gccgacgaca tgcgcgagca
     2761 gctcattagc tcgatccaga agaatacgga gatcgacgag ccggaggccg gcgcggcgcc
     2821 gatcgtcgcc acggaccgcc gtggccagct catcggccgc gacgaccaga gcgccgacat
     2881 ggctgtcgat cacatcacca tctccgagga gcagctcctg cgcgggatgg agagcatgac
     2941 cccgatggag ccgtcggcaa gcggcaacgg gggtggctcg ggcggttcgg gaagccgcgg
     3001 ccgctgagtt cggtcggcgg gccgacggcc gccggcaagc ccgccagatc cgatcgcgat
     3061 acgtcgtgcg gccgcctcgt cgtcgcgccg gggaaccgtt atacttggcg gtgcgtgctc
     3121 ccggattcga gtaccggcgg tgacatgaca tgatgcggca gttcgatctc gtcgaaagag
     3181 tgcagcggta cgatccgacc gccgataccg acttgctcaa ccgcgcctac gtctatgcca
     3241 tgcgcaagca tgggaaccag aagcgcaagt cgggtgaact gtatttttcg cacccgctcg
     3301 aggtggcggc gattctgacc gacctccgac tcgacgatgc gacgatcgcg actgccctcc
     3361 tccacgacgt catcgaagac acggccacca cccgcgacga gatcgacagt ctgttcgggt
     3421 ccgagatcgg ggcgctggtc gaggggctga ccaagatcaa gcggctcgac ctcgtcaccc
     3481 agaaggccga gcaggcggag aacttccgca agctgctggt cgcgatctcg accgacatcc
     3541 gcgtcctgct cgtgaagctc gcggatcgcc tccacaacat gcgcacactc gagcacaatt
     3601 cgcccgagag ccgcaagcgc aacgccgagg agacactcga gatctacgcc cccctcgccg
     3661 gccgcatggg catgcaggcg atccgcgagg agctcgagga cctgtcattc cgctggctca
     3721 atccggaggc ctacgcaacg gtcaccgaaa agctcggcca gctgcgcgag cgcaaccagg
     3781 ggctcgtaga cgagattgcg ggcgccttgc gcgagaagct cgctggtctg ggcatacggg
     3841 ccgaggtgtc gggtcgcgag aagaagccct atgccatctg gcgcaagatg gtgaacaagc
     3901 agatcagtct cgaacagctt tccgacatct acgccttccg cgtctctgtc gagcagcgcc
     3961 aggaatgcta cggcgtgctc ggcgtgatcc actcggcctg gcgggcggtg cccggccgtt
     4021 tcaaggacta catctcgacg ccgaagcaga acggctacca gtcgatccac acnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnngag ggcggcaagg cagcgatcga gcgagcgccg ggcgaaagcg
     4201 acgggcgcga cggaccgtac atgtggctgc gcagcctggt cgacacactt ctcaaggggg
     4261 acaatccgga ggagtttctc gagcacacga agctcgagct cttccaggac caggtgttct
     4321 gcttcacgcc gaaggggcgg atcatcgcgc tcccgcgcgg cgcgacgccc atcgatttcg
     4381 cctacgccgt gcacaccgat gtcggcaact cgtgcgtcgg
//
LOCUS       DAORNH010000347         4419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14746_length_4419_cov_2.898946, whole genome shotgun sequence.
ACCESSION   DAORNH010000347 DAORNH010000000
VERSION     DAORNH010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14746_length_4419_cov_2.898946"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PK264_22960"
     CDS             complement(<1..557)
                     /locus_tag="PK264_22960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78761.1"
                     /translation="MTASFEAKSDALPQSTGCGKTVSRCRSAVRFPPSVVKAGLAFGL
                     VLGVAGSLSMSSATSQDRSGAVPTPVVIGLLEDVPSAKALCEPEQMLELAGEAVDDVK
                     DVRAPIHGHVLQFVNPTTDGRQETFYVGVYSYRSTEWVVNNSAIILKVVGAQISLSLR
                     SARLTSYLECGGIYTVRAPSVNMCLP"
     gene            complement(685..1470)
                     /locus_tag="PK264_22965"
     CDS             complement(685..1470)
                     /locus_tag="PK264_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78762.1"
                     /translation="MCDRSAGLTPTDRGPIVRWTWRRLGAGVAGLGLALSVLGLLGRQ
                     EAVAQRGELIMRPAGVLDDVKSVKDFCDPKRLAGLLGPPVEDAEDVDAPVHGVAFQFI
                     VTSGAPNTKESFYIAIFSYMNLEALANQTTMLLAQLGIPPEKARLTNYERCAPLFTSV
                     AGKSSKAHQPLLAMVKRQTLLGESVQIIPVAALYLSHSGALAGLLVPPKDAAPPKADA
                     PPAVVTPKPDTPPSVPPKTDAAPPPKSDTLPSKPVIIPVKPIR"
     gene            complement(1616..2665)
                     /locus_tag="PK264_22970"
     CDS             complement(1616..2665)
                     /locus_tag="PK264_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78763.1"
                     /translation="MSTLKSKLMAAGLAGLAVAGVAGQAQAQNRAPTVLVMTEDADPK
                     AVARSNRMMKRVYDAMMDELMSKGYSVMHENRLTMDFTTKPGERRTERELLQIARLVK
                     QPPIDIVVSYEIYAVVEQVPYTNKDPVYRANVSISGRAIAVASGRFLGAFDVGPQDIG
                     PLAKSCIGQDHCLLESVGAETRVMGRDLAHAIGNKVAIGMGDGGRVPPVGGPIPGVVG
                     QPMPGPGPVGPGPVAGLPPVGMPGPGPMGQPGCAGFVADFSISIEGFTATELTAMEEY
                     LASFSCFISKRVVPPSGPSRTNFWYTTQSDEARLFRNLRLMLSQMNLLERQDKIDHAI
                     IERIGDNKIRVRKLK"
     gene            complement(2806..3684)
                     /locus_tag="PK264_22975"
     CDS             complement(2806..3684)
                     /locus_tag="PK264_22975"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD78764.1"
                     /translation="MSSSLESPNFQEITMMGLSIAKRKGRAGLMAALVLASLTVPVTP
                     SFAQQDAAAKRATDVLVRSITPGSRHDIGYGHMSRRIPIISDGGTTSHVIVDYRYQHD
                     VTLFFDYDSYEITDRAARQLRHLGYALSSPETLPYRYLIAGHTDGKGSAEYNQKLSLK
                     RAMAVRDHLVKVYHIDPYRLIVTGWGFSQLKVPSNPYASVNRRVEVVLVADPSYAPNP
                     QPNADYRIPGPVYGHIPMPGVAAPIPCPPGGVPSVIPDPRGGRSLDDFGRGRTPVGCD
                     PAPVIAPPVTPPGNYK"
     gene            complement(4081..>4419)
                     /locus_tag="PK264_22980"
     CDS             complement(4081..>4419)
                     /locus_tag="PK264_22980"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRD78765.1"
                     /translation="FGVEPMSPVPVGSYPANAFGLFDMHGNAAEIVADCYADQHTGLP
                     ANGSARASCRDARYVLRGGSWQMLKLRIRSSWRDFVEPGLRREDIGFRVARDIAPAEG
                     DTPAPTACPR"
BASE COUNT          793 a         1375 c         1512 g          739 t
ORIGIN      
        1 ggcaggcaca tgttgaccga cggcgcgcga acggtgtaga tgccgccgca ctcgaggtag
       61 gaggtcagac gcgcggagcg cagcgacagg ctgatctgcg cgccaacgac cttcaggatg
      121 atggcggagt tgttcaccac ccattcggtc gagcgatacg aataaacccc gacgtagaaa
      181 gtctcctgac ggccatcggt cgtgggattg acgaactgca ggacgtgccc gtggatcggc
      241 gcccggacat ccttgacatc gtcgaccgct tcgccggcaa gctccagcat ctgctccggc
      301 tcacagagtg ccttggccga aggcacatcc tcgagaagcc cgatcacgac cggtgtcggc
      361 accgcgcctg agcggtcctg gctggtcgcc gaggacattg agagcgagcc ggcgaccccg
      421 agcacgaggc cgaacgccag cccagccttc accaccgacg gcggaaatcg gactgcgctg
      481 cgacagcgcg agaccgtctt cccgcatcca gttgattgcg ggagcgcatc actctttgcc
      541 tcaaatgaag ccgtcacggg tgcacctcgc ttggcccttc accctcccgg accgtgccgt
      601 gacaggcctg tccggtgctc agctcgtcaa acactggaag catactgcac cggtcggtcg
      661 tggcaaacaa acttagcgta acagctagcg tatcggcttc accgggatga tcactggctt
      721 cgagggcaga gtgtctgact tcggcggcgg agcggcatcc gttttcggcg gcacggaagg
      781 cggcgtatcg ggcttcggcg tcacgactgc gggcggagca tctgccttcg gaggggccgc
      841 atccttcggc ggcacgagga ggcctgcgag cgcgccgctg tgcgagagat agagtgctgc
      901 gacgggtatg atctgcacgc tctcgccgag caaggtctga cgcttcacca tggcgagcag
      961 cggctgatgc gccttcgaac tcttgcccgc aacgctcgtg aagaggggcg cacagcgctc
     1021 gtagttcgtc agccgagcct tctcgggcgg gatgccgagc tgggcgagca gcatcgtggt
     1081 ctggttcgct aacgcctcga ggttcatgta ggagaagatg gcgatataga agctttcctt
     1141 cgtgttcggt gcgccgctcg tcacgatgaa ctggaacgcg acgccgtgga ccggagcatc
     1201 cacgtcctct gcatcttcga cagggggacc gagcagcccc gcgaggcgct tcgggtcgca
     1261 gaagtccttc acgctcttga cgtcatcgag tacgcccgcg ggccgcatga tgagctcgcc
     1321 gcgctgcgca accgcctcct ggcggccgag gaggccaagc acggagagcg cgaggccaag
     1381 cccggcgacg ccggcgccga gccgccgcca cgtccagcgg acgattgggc cgcgatcggt
     1441 tggagtgagg cctgctgacc gatcgcacac agggcacctc tgacagcaac agttgttcag
     1501 gaattcggca gtccggcaga gtgccacaac gcgtcagcac ccaccaacga cgttcaaccg
     1561 acgaccaaag cggcggaccc gttaggatcc gccgccctgg cttcgagtta caggcttact
     1621 tcagcttgcg gacgcggatc ttgttgtcgc cgatccgctc gatgatggcg tggtcgatct
     1681 tgtcctggcg ctcgagcagg ttcatctgcg agagcatcag tcggaggttg cggaagaggc
     1741 gagcctcgtc agactgcgtc gtgtaccaga agttggtgcg cgacggacca gagggcggca
     1801 ccacgcgctt cgagatgaag caggagaagg acgcgaggta ctcttccatc gccgtcagct
     1861 cggtggccgt gaagccttcg atgctgatcg agaagtcggc cacgaagccg gcgcaaccag
     1921 gctgacccat cgggccgggg cccggcatgc caaccggcgg gagaccagcg accgggccgg
     1981 gaccgaccgg accagggccc ggcatcggct gaccgacgac acccgggatc gggccgccga
     2041 ccgggggaac gcgaccgccg tcacccatgc cgatggctac cttgttgccg atggcgtgag
     2101 cgaggtcacg gcccatcacg cgtgtctcgg cgccgaccga ctcgagcagg cagtggtcct
     2161 ggccgatgca cgacttggcg agcggaccga tgtcctgcgg accgacgtcg aaggcgccga
     2221 ggaagcgacc ggaggcaacc gcgatggcgc ggcccgagat ggagacgttg gcgcggtaga
     2281 cggggtcctt gttggtgtag ggcacctgct cgacgacggc gtagatctcg taggagacga
     2341 cgatgtcgat cggcggctgc ttcacgaggc gggcgatctg gaggagctca cgctcggtcc
     2401 ggcgctcgcc gggcttcgtc gtgaagtcca tcgtcaggcg attctcgtgc atcacggaat
     2461 agcctttgga catcagctcg tccatcatcg cgtcgtagac gcgcttcatc atgcggttcg
     2521 agcgggcaac ggccttcggg tcggcatcct cggtcatcac gagaacggtc ggagcgcggt
     2581 tctgggcctg ggcctggccc gccactccgg cgacggcaag accggcaaga ccggctgcca
     2641 tcagcttgga cttcagggtg gacatcgtca ttccttcctt tgtgggttgg cagtcagaag
     2701 aacttggaca gttattggag aaagcagagc gacttttcgg gttttctcgt cttccttctg
     2761 aggacacctt cccgccggga atagtgccct cagaaggggc attcgtcact tataatttcc
     2821 gggcggggta accggcggag cgatcactgg agccgggtcg cagccgaccg gcgtccgtcc
     2881 acgtccgaag tcgtcaagcg agcggccacc gcgcgggtcg gggatgaccg agggcacacc
     2941 gcccggcggg caggggatcg gagcggcgac gcccggcatc gggatatggc catagaccgg
     3001 gcccgggatg cggtagtcgg cgttcggctg tgggttcggc gcgtaggacg ggtcggcgac
     3061 gagcacgacc tcgacacgac ggttcaccga ggcgtagggg ttcgagggga ccttcagctg
     3121 gctgaagccc cagccggtga cgatcagccg gtagggatcg atgtggtaca ccttgacgag
     3181 gtggtcacgc acggccatcg cgcgcttcag cgagagcttc tggttgtact cggcggaacc
     3241 cttgccgtcg gtgtggcccg cgatcaggta gcggtaggga agcgtctctg gcgaggagag
     3301 cgcgtagccg agatggcgga gctggcgcgc ggcccggtcc gtgatctcgt agctgtcgta
     3361 gtcgaagaac aaggtgacgt cgtgctgata gcggtagtcg acgatcacgt ggctcgtcgt
     3421 gccgccatca gagatgatcg ggatgcggcg cgacatgtgg ccgtagccga tgtcgtggcg
     3481 gctgcccgga gtgatcgagc gcacgagaac gtcggttgcg cgcttcgctg ccgcgtcctg
     3541 ctgagcaaag ctcggcgtga ccggcacggt gagcgacgcg aggaccagcg cggccatcag
     3601 gcccgcccgt ccctttcgct tggcaatgga aagacccatc atggtgatct cctggaagtt
     3661 ggggctctcg agggacgagc tcacgaccct ctttcagctg attgcgtccg gcagaccaga
     3721 ttttccggat gtctcgagac gcttgaagcc ctcctgttca ttcagggttc agcccggcat
     3781 agagcgcgcc cctcaatatg cgcacgggtg ctcgatctcg tgtcgcgcaa gaagcttgcc
     3841 agaagggggg ccggcatcga ccggcaggcc ccgtgctcgg gacatggttg cagcccatgc
     3901 cggcaccgat ccggacaatc gcgcatagag ggcagccgac cacgctcgct gcacatctcg
     3961 gcgtctccgc agacgatcgg acagcgagcc aggcatcggc atctccacgg caatggcagt
     4021 cggcgatcaa tcgagttgcg gaagcaatga agcggcctct ctggggaagc cgctccaaga
     4081 tcagcgcggg caggctgtcg gtgccggagt atcgccctcg gcaggtgcga tatcgcgggc
     4141 gacgcggaag ccgatgtcct cacgcctcaa ccccggctca acgaaatcgc gccaggaact
     4201 cctgatccgg agcttcagca tctgccagct gccgccacgc agcacatagc gggcatcgcg
     4261 gcagctcgca cgcgccgagc cgttggccgg cagcccggta tgctggtcgg cgtagcagtc
     4321 ggcgacgatc tcggctgcat tgccgtgcat gtcgaagagt ccgaaggcgt tggcaggata
     4381 gctgccgacc ggcaccgggc tcatcggctc cacgccgaa
//
LOCUS       DAORNH010000348         4413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14779_length_4413_cov_2.752868, whole genome shotgun sequence.
ACCESSION   DAORNH010000348 DAORNH010000000
VERSION     DAORNH010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14779_length_4413_cov_2.752868"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK264_22985"
     CDS             <1..242
                     /locus_tag="PK264_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78766.1"
                     /translation="AGIETRLWDMTDIVALIAGRIGHDRAGDPCYQDRAREWDQQGSS
                     HHRLDHRLGRPPAVRSLPALRRTIYSRQRANAYPS"
     gene            255..1079
                     /gene="map"
                     /locus_tag="PK264_22990"
     CDS             255..1079
                     /gene="map"
                     /locus_tag="PK264_22990"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRD78767.1"
                     /translation="MNLQLSRATRAPEGTVTLHGPEAFEGMRRSGRLAAAALDMITPF
                     VVPGVTTERLDDLVTEFARDNGAIPAPLNYRGFPKAICTSVNHVVCHGIPGPKPLREG
                     DIVNIDVTLILDGWHGDTSRMYPVGAISRRAERLIEVTHQALMIGIAAVRPGNWTGHI
                     GTAIERFARSQRCSVVRDFCGHGLGRVFHDRPNILHYQDTNDGVALQPGMLFTIEPMI
                     NLGRADVKVLHDGWTAVTRDRDLSAQFEHTVGVTATGCEVFTLSPAGLDRPPYGKA"
     gene            1117..1914
                     /gene="radC"
                     /locus_tag="PK264_22995"
     CDS             1117..1914
                     /gene="radC"
                     /locus_tag="PK264_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRD78768.1"
                     /translation="MAEPSPLPAAQSSERPQSGEAPQLALWPGEVLPLPREAPAAAAP
                     LAAVSGHEGHRRRLRERFEKGGAGPIPDYELIELLLFPVLPRRDTKPIAKALLARFGS
                     LSEVLAAPAERLQEIEGIGPSAALHLAVVRAAKSEIAPRKVLGSWTAVIDYCRAAQGF
                     DTQESFRILFLDKRNQLIADEQQSRGTVDHTPVYIREVVRRALELASTALILVHNHPS
                     GDTTPSRADVDMTRQIIEAGKPIGIAVHDHIIVGRSGHASLRGLKLI"
     gene            complement(1938..3113)
                     /locus_tag="PK264_23000"
     CDS             complement(1938..3113)
                     /locus_tag="PK264_23000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRD78769.1"
                     /translation="MVLTTSFEKSPTKDVRPLSLPKRYRVAVERSPRHWLAAADAEQG
                     LVRTPFQAPFWLEAWYHTLGASGEREPVLVTARDETSGQIALLLPWMLSRQSGLRVVE
                     PADGGVSDYNGPILGPAAPQDAVGAADLWRDIKAALPPADLVRIEKMPREIAGKANPL
                     ALISSVKNSPLWGHVINIEGTFLEFLASRGKHFRKEIQRSRRVLEREGCVSFRAITDP
                     GEAQMVFDTLEAYQGARMREAGVDYRLDEPATRAFYRRALADGLGSGEAQLFSLEVDG
                     VIVAAVFGIADQSAFLLLRIANAGERWRHCSPGRLIAAEIIEWLMAKEIRCIDMTIGD
                     YAFKRQFDPKPYPLVELVEALSWRALPVIARHRARDLVHRNERLLGIVRRVRGRIKG"
     gene            complement(3118..4089)
                     /locus_tag="PK264_23005"
     CDS             complement(3118..4089)
                     /locus_tag="PK264_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78770.1"
                     /translation="MRPAITEASPGALAAPLFTDWQGKHAELWANQAVRLQHRAHLLD
                     LFSRDSLAELIDRYPRQHYALVHMGAQGDRRLWREGDLAGMPGHKVIEAIANGRMWLN
                     LRRVSEVDRRFGALLDQIFAEVERRVPGYATFSRTSGILISSPMAQVYYHSDLPGQSL
                     WQIEGRKRVYVYPPKEPFLSGAQLENIALYGIEVDLAYEPWYDEHAEMFEIGPGEMLH
                     WPLNAPHRVENLDCLNISMTTEYWTEAIRRRQMVNLANGIMRNSLRLAPRSRTIAGPT
                     FWAKAMLQAGVKRAGLLEKPRKARRPIDFRLDPEHPGAIVDLATAAE"
BASE COUNT          764 a         1479 c         1460 g          710 t
ORIGIN      
        1 ccgccgggat cgaaacgcgt ttgtgggaca tgaccgatat cgtcgcgctg atcgcggggc
       61 ggatcggcca cgaccgggcc ggcgacccat gctaccagga tcgcgcccgc gagtgggatc
      121 agcaaggctc gtctcatcat cgcctcgacc atcgcctggg ccgtccgccg gccgtccgat
      181 ctttacccgc cctgagacgc acaatatata gtcgccagag agcaaacgcc tacccgtcat
      241 gaggaacgcg cgcgatgaac ctgcagctgt ctcgagcgac gcgcgcgccg gaaggcaccg
      301 tcacgctcca cggccccgaa gccttcgaag gcatgcgacg gtcgggccgt ctcgcagccg
      361 ctgccctcga catgatcacg ccgttcgtgg ttcccggggt caccaccgag cgcctcgacg
      421 atctcgtcac ggagttcgcg cgcgacaacg gcgccattcc cgctccgctc aactatcgcg
      481 ggttcccgaa ggcgatctgc acatcggtca atcacgtcgt ctgccatggc attccaggcc
      541 ccaagccgct gcgcgaaggc gatatcgtca acatcgacgt cacgctaatc ctggacggct
      601 ggcacggtga cacgagccgc atgtacccgg tcggcgccat ctctcggcgc gccgagcgcc
      661 tgatcgaggt gacccaccag gccctgatga tcggcattgc ggcggtgaga cccggcaact
      721 ggacgggcca tatcggcacc gcgatcgagc gcttcgcgcg ctcgcagcgc tgcagcgtcg
      781 tgcgcgactt ctgcggccac ggcctcggcc gcgtcttcca cgaccggccg aacatcctcc
      841 actaccagga caccaatgac ggcgtcgccc tccagcccgg catgctgttc accatcgagc
      901 cgatgatcaa cttgggccgc gccgacgtga aagtgctgca tgacggctgg acggccgtga
      961 ctcgggatcg cgatctctcc gcccagttcg agcacacggt cggcgtgacg gcgacgggct
     1021 gcgaggtgtt cacgctgtcg cccgccggcc tcgaccggcc gccgtacggc aaagcctagg
     1081 gggcacgaga tgcgacgcgg caagccgcca agtggcatcg ccgagccgtc gccgctgcct
     1141 gctgcgcaat cgtccgagcg cccgcagtcc ggggaggcac cgcagcttgc gctgtggccg
     1201 ggagaggttc tgccattgcc acgagaggcg cccgccgcgg cggcaccact cgcggccgtg
     1261 agcggccacg aaggccatcg ccgcaggctc cgtgagcgct tcgagaaggg tggcgcggga
     1321 ccgattcccg actacgagct catagagttg ctactctttc cggtgctgcc gcgacgcgac
     1381 acgaagccga tcgccaaggc gttgctcgcg cgattcggct ctctttcgga ggtactcgcc
     1441 gcgccagcgg agcggctcca ggagatcgaa ggcatcgggc cgtcggcagc actccatctc
     1501 gcggtcgtgc gtgcggcgaa gagcgagatt gcgccccgca aggtgctcgg gtcctggacc
     1561 gccgtgatcg actactgccg ggccgcacag ggcttcgaca ctcaggagag cttccgcatc
     1621 ctctttctcg acaagcgcaa ccagctgatc gccgacgagc agcagagccg gggtacggtt
     1681 gatcacacgc cggtctacat ccgtgaagtg gtccggcgtg cgctcgagct tgcctcgacc
     1741 gcactcatcc tggtccacaa tcatccctct ggcgatacga ctccgtcacg cgccgacgtc
     1801 gacatgacgc ggcagatcat cgaggccggc aagccgatcg ggattgcggt gcatgaccac
     1861 atcatcgtcg gccgcagcgg acatgcgagc ctgcgcggct tgaagctcat ctgacgggat
     1921 ggcgacggca gcctcgctca gcctttgatg cgcccacgca cgcgacgcac gatgccgagc
     1981 agccgctcgt tgcggtgcac caggtcgcgc gcgcgatggc gtgcgatgac gggtagcgcg
     2041 cgccaggaga gcgcctcgac cagctcgacg agcgggtagg gcttgggatc gaactggcgc
     2101 ttgaaggcgt aatcaccgat cgtcatgtcg atgcacctga tctccttggc catcagccat
     2161 tcgatgatct cggcggcgat gagccgaccc ggcgagcagt gcctccagcg ctcgccggca
     2221 ttggcaatgc gcagcagcag aaatgcgctc tggtcggcaa taccgaatac cgccgcgacg
     2281 atgacgccat cgacttccag cgagaagagt tgcgcctccc cgctcccgag gccatccgcc
     2341 agggcgcgtc ggtagaaggc gcgcgtcgcg ggctcatcga gccggtagtc gacgccggcc
     2401 tcgcgcatgc gggcgccttg gtaagcctcg agcgtatcga acaccatttg cgcctcacct
     2461 ggatcagtga tcgcacggaa cgagacgcag ccctcgcgct cgaggacacg gcggctgcgc
     2521 tggatctcct tgcggaagtg cttgccgcgg cttgcgagaa actcgaggaa cgtgccctcg
     2581 atgttgatga catggcccca gagggggctg ttctttacgc tggagatgag cgcgagcggg
     2641 ttggccttcc cagcgatctc gcgcggcatc ttttcgatgc ggacgaggtc ggcaggcggc
     2701 aaagcagctt tgatgtcgcg ccagagatcg gccgcaccga ccgcatcctg cggcgcggcg
     2761 ggccccagga tcgggccatt gtagtcgctg acgccaccat cggccggctc gacgacacgg
     2821 aggcccgatt gccgcgacag catccagggc aggaggagcg cgatctggcc gctcgtctcg
     2881 tcgcgcgccg tcacgagcac cggctcgcgc tccccactcg cgccgagggt gtgataccag
     2941 gcctcgagcc agaacggcgc ctggaacggc gtgcggacga ggccctgctc cgcgtctgca
     3001 gcggcaagcc agtggcgggg cgagcgctcg accgccacgc gatagcgctt tggcagcgac
     3061 aatggccgca catccttcgt tggcgacttt tcgaaactcg tcgtgaggac cacgcgacta
     3121 ctcggccgcc gtggcgagat cgacgatcgc gccgggatgc tcgggatcga ggcggaagtc
     3181 gatcggccgg cgtgccttgc gcggcttctc gaggaggccc gcccgcttga caccggcctg
     3241 cagcatggcc ttggcccaga acgtcggacc ggcgatggtg cgactgcgcg gcgcgaggcg
     3301 caacgagttc cgcatgatgc cgttggcgag gtttaccatc tggcgtcggc ggatcgcctc
     3361 cgtccagtac tcggtcgtca tcgagatgtt gaggcaatcg aggttctcga cgcggtgcgg
     3421 ggcgttgagc ggccagtgca gcatctcgcc aggaccgatc tcaaacatct cggcatgctc
     3481 gtcgtaccaa ggctcgtatg cgaggtcgac ctcgatgccg tagagcgcga tgttttcgag
     3541 ctgcgctccg gagaggaacg gctccttcgg cggatagacg tagacgcgct tgcgcccctc
     3601 gatctgccag agcgactggc cgggaagatc ggagtggtag tagacctgcg ccatcggcga
     3661 ggagatcagg atgccgctcg tgcgcgagaa cgtggcatag ccaggaacgc gccgctcgac
     3721 ctccgcgaag atctgatcga gcagagcgcc gaagcggcgg tcgacctcgg acacccggcg
     3781 cagattgagc cacatgcggc cattggcgat cgcctcgatc accttgtggc ccggcatgcc
     3841 ggccagatcc ccctcgcgcc agagccgccg atctccctgc gcgcccatgt gcacgagcgc
     3901 atagtgctgt cgcggatacc ggtcgatgag ctcggccagg ctgtcgcgcg agaacaaatc
     3961 aagaagatgg gcgcgatgct gcaggcgcac ggcctgattg gcccagagct cggcgtgctt
     4021 tccctgccaa tcggtgaaga gaggggccgc gagggcgcca ggcgaggctt ccgtgatcgc
     4081 tggtctcaca gggtcgggcg gcggccgccg gtcgcaggac gtttcgaatt cggacgcgcc
     4141 atcattgcgc tacccgtata caattttcga gccaagcgag gcaagattgc gcgatccatg
     4201 gatttcttga cctgagctgc ggcaaacgcg tttgcccctg agtggtgtcc ccgttgcggc
     4261 cacaatcaaa tcagatacct ggggccagtc gaggcacgga cggccagtcg aggccggaag
     4321 tcgagcgtgc cgaaccgatc aacaaggaca cggtgaagat gatcaagacc ttcctcgtct
     4381 cgctcggcct cagcgccgtc ctggcgacag cgg
//
LOCUS       DAORNH010000349         4403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14836_length_4403_cov_2.324287, whole genome shotgun sequence.
ACCESSION   DAORNH010000349 DAORNH010000000
VERSION     DAORNH010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14836_length_4403_cov_2.324287"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>405
                     /locus_tag="PK264_23010"
     CDS             <1..>405
                     /locus_tag="PK264_23010"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD78771.1"
                     /translation="RVVQAELAATLKRIAAEGSDALYGGSIGRRVAENIQAQGGTVSL
                     ADLSSYAIVEREPIRAAYRGRTIIGPPPPASSGVHIAEMLNILESDDIAAMGFGSADA
                     VHLLAEALKIAFADRAVATADPAFVSVPVERLT"
     assembly_gap    406..488
                     /estimated_length=83
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <489..1077
                     /locus_tag="PK264_23015"
     CDS             <489..1077
                     /locus_tag="PK264_23015"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD78772.1"
                     /translation="SANTTHVTVADADGHVVSATQTINGVFGAAVMIPGTGLIANNYM
                     HNFDPHPGRALSIAPGKRVFTSMSPMMIAEDGQVRHALGLPGGLRIFPSAFQAIVNLI
                     DHGMPAQEAVEAPRVWTEGGPLELEPAFGAEISADLEARGHRLERGNRVAGGMNAISI
                     GDDGILTGAACWRADGTPVGIAGGLARPGVRFTLS"
     gene            1095..>1781
                     /locus_tag="PK264_23020"
     CDS             1095..>1781
                     /locus_tag="PK264_23020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD78773.1"
                     /translation="MTYTVAVLDVITPPRAAELRALLPAGFVLTHGTKPGIEHMKETI
                     RDADFAISAQVAVTREVLAAARRLKLLHKWGVGVDNFDVEAARDLGIGVARTTGSNAL
                     SVAEFTIGLMLSTLRFSAFGHHHLKQGDWRGISNLPAPTFLLSGKTVGVVGFGAIGQN
                     VARLLKGFGCRVLFSKRTPLPAEEAEALGATHTTMDTLLAESDVVSLHCPLTPATTNL
                     IDTAALQLMKR"
     assembly_gap    1782..1791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1792..2067
                     /locus_tag="PK264_23025"
     CDS             <1792..2067
                     /locus_tag="PK264_23025"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD78774.1"
                     /translation="ILVNVARGGVVVEPDLAAALRARTIRGAAMDVFSIEPLPATSEL
                     LALDNIVVTPHLAAVTADTFEPTVRRMYENMARITRGEPVPEKDRVV"
     gene            complement(2071..2862)
                     /locus_tag="PK264_23030"
     CDS             complement(2071..2862)
                     /locus_tag="PK264_23030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78775.1"
                     /translation="MSEDAGPLLKVLAVLVYALLLAPVAIVLVISFSADTFIVFPPSG
                     YSLRWYERVFANQVLMQGLKLSAILALVVAGLSLLVGLPAAYAIARGLVPGAAVLKAF
                     FLAPLLLPTLVVGLALLMALQPWRLTATLTGLALGHMVVTVPFVIRLMVTTFATLPQE
                     VEAAAASLGATPWRVFRRVTLPLALPGLAASAFLAFLLSFDETVISLFLSGPRASTLP
                     VELVRYVEGRTDPLVAALSVILIAGTIGVVLIVERLVGVVRVIGK"
     gene            complement(2859..3722)
                     /locus_tag="PK264_23035"
     CDS             complement(2859..3722)
                     /locus_tag="PK264_23035"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78776.1"
                     /translation="MSGLERSPLARLIGVLLVVPAVAVLVLLYVVPMAGLGRMAFNET
                     GPTGAMVEAWSLVTFRATLGDSFTLELTWNSIWLSLVSTSLALFLAYPLALFLFRTRS
                     RFKALLTVLAVAPMLVSGVVRVFGWLALLGDRGLVNAALMGSGLTSEPTRLVFNYFGV
                     SIGLAESLMPYMILALISGFGRLDRACEEAAASLGATPWRVFRRVTLPLSLPAVILGF
                     LLCFVLAMSAYVTPKLLGGGRVFLLATEIFEHANTNVNWPAAAALAIYSLALIGLLTA
                     AYSVLTRRSER"
     gene            complement(3719..>4403)
                     /locus_tag="PK264_23040"
     CDS             complement(3719..>4403)
                     /locus_tag="PK264_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD78777.1"
                     /translation="LAARKPRELSGGQQQRVALARALVIEPDVLLLDESLSNLDAKLR
                     DAMRLEIRDLQRALSLTTLFVTHDQTEALTMCDRIAVMNAGRIEQVASPRDIYDRPAS
                     RFVADFVGRSNVLNAAPDASGRLALAGRALRLAKAPPPGSVEIFLRPQRLMLREAADA
                     LPDALNGLAGRIAASMFVGDRIELAVETEAGRLIVELPHAADAPPVGREVMIAWRPED
                     TLAFAVPAT"
BASE COUNT          772 a         1488 c         1477 g          573 t
ORIGIN      
        1 cgcgtcgtgc aggccgagct tgcggcaacg ctcaagcgca ttgcggcgga gggctccgac
       61 gcactctacg gcggctcgat cggccgtcgc gtcgccgaga atatccaggc gcagggtggc
      121 acggtcagtc tcgccgacct ctcgagctac gcgatcgtcg agcgcgagcc gatccgcgcc
      181 gcctaccgcg gtcgcacgat catcgggccg ccgccgccgg cctcgagcgg cgtgcacatc
      241 gctgagatgc tgaacatcct cgagagcgac gacatcgctg ccatgggctt cggctcggcg
      301 gacgccgtgc acctcctcgc cgaggccttg aagatcgcct tcgccgaccg tgccgtcgcg
      361 accgccgatc ccgccttcgt ctcggtgccg gtcgagcgtc tgacgnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnngt cggccaatac cacccacgtc acggtcgccg acgccgacgg ccacgtggtc
      541 agtgccacgc agacgatcaa cggcgtcttc ggcgcagccg tgatgatccc ggggacgggc
      601 ctcatcgcca acaactacat gcacaacttc gacccgcatc ccggccgcgc gctctcgatc
      661 gcgccgggca agcgcgtctt cacgtccatg tcgccgatga tgattgccga ggacggccag
      721 gtgcgtcacg cgctcggtct gccgggcgga ttgcgcatct tcccctccgc cttccaggcg
      781 atcgtcaatc tgatcgacca cggcatgccg gcgcaggagg ccgtcgaggc gccgcgagtg
      841 tggacggagg gcggcccgct cgagctcgag ccggcgttcg gcgcggagat ctccgccgat
      901 ctcgaggctc gcggccatcg gctcgagcgc ggcaatcgcg tcgccggcgg catgaacgcc
      961 atctcgatcg gcgacgacgg tatcctcacc ggcgctgcct gctggcgcgc ggatggcaca
     1021 ccggtcggca tcgcaggcgg cctcgcgcgg ccaggcgtcc ggttcactct ctcataaggc
     1081 aagcccaagg catcatgacc tacaccgtcg ccgtccttga cgtgatcact ccgccgcgcg
     1141 cggcggagct gcgtgcgctg ctgccagcgg gcttcgtgct gacgcacggc acaaagcccg
     1201 gcatcgagca catgaaggag accatccgcg acgcagactt cgcgatctcg gcccaggtcg
     1261 cggtgacgcg cgaggtgctc gctgccgcac ggcgcctgaa gctcctccac aagtggggtg
     1321 tcggtgtcga caatttcgac gtcgaggcag cgcgagatct cggcatcggc gttgcccgca
     1381 cgacgggcag caacgcgctc tcggttgcgg agttcacgat cgggctgatg ctgtcgacgt
     1441 tgcgcttctc ggcattcggg caccatcatc tgaagcaggg cgactggcgt ggcatcagca
     1501 atctgcccgc gccgacgttc ctgctctccg gaaaaaccgt cggggtcgtc ggcttcggcg
     1561 cgatcgggca gaacgtggcg cggctgctca agggattcgg ctgccgcgtc ctcttttcga
     1621 agcgcacgcc gctgccggcg gaggaggcag aggcacttgg cgccacgcac acgaccatgg
     1681 atacgctgct ggcggaatcc gacgtcgtct ccctgcattg cccgctgaca ccggcgacga
     1741 cgaacctcat cgatacggcc gcactccagc tcatgaaacg gnnnnnnnnn natcctcgtc
     1801 aacgtggcgc gcggcggcgt ggtggtcgag cccgatctcg ctgcggcctt gcgcgcccgc
     1861 acgatccgcg gcgcggccat ggacgtattc tcgatcgaac cgctgccggc gacgagcgag
     1921 ctgctcgccc tagacaacat cgtcgtgacg ccgcaccttg cggccgtcac cgccgatacc
     1981 ttcgagccga cggtccgtcg catgtacgag aacatggcgc gcatcacgcg cggcgagccg
     2041 gtgcctgaga aggaccgcgt cgtctgaaga tcacttgccg atgacgcgca cgacgccgac
     2101 gaggcgctcg acgatcagca cgacgccgat cgtcccggcg atcaggatca ccgaaagcgc
     2161 cgcaaccagc ggatcggtgc ggccctcgac gtagcgcacg agttcgaccg gcagggtcga
     2221 ggcgcgcgga cccgacagga acagcgagat caccgtctcg tcgaacgaca gcaggaaggc
     2281 caggaacgcc gaggccgcga gccctggcag cgcgagcggc agcgtcacgc gccggaacac
     2341 gcgccagggc gtcgcgccga ggctcgctgc tgcggcctcg acctcctgcg gcagggtcgc
     2401 gaacgtcgtt accatcagcc ggatgacgaa cggcactgtc accaccatat gaccgagggc
     2461 aaggcccgtc agcgtcgccg tgagccgcca cggctgcagc gccatgagga gcgcaaggcc
     2521 gacgaccagc gtcggcagca gcaaaggcgc aaggaaaaag gccttgagta ccgcggcacc
     2581 gggcacgagc ccgcgcgcga tcgcgtaggc ggccggcagc ccgaccaaca gggagaggcc
     2641 cgcgacgaca agtgccagga tggccgagag cttcaggccc tgcatcagca cctgattggc
     2701 gaagacgcgc tcgtaccagc gcagcgagta gccggacggc gggaagacga tgaaggtgtc
     2761 ggccgagaac gagatcacca gcacgatggc gacaggggcg agcagcagcg catagacgag
     2821 cacggcgagc accttcagca gcggccccgc atcctcactc atcgctcgct ccgtcgcgtg
     2881 aggacactgt aggcggccgt cagcagcccg atcagcgcga gcgaatagat cgcgagcgcg
     2941 gcggcggccg gccagttcac gttggtgttc gcgtgctcga agatctcggt cgcgagcagg
     3001 aacacgcgcc cgccgccaag gagcttcggc gtaacgtagg cgctcatggc cagcacgaag
     3061 cagagcaaga aaccgaggat cacggcgggc aggctcaatg gcagcgtcac gcggcggaag
     3121 acgcgccacg gcgtcgcccc gaggctcgcc gccgcctcct cgcaggcccg gtcgagccga
     3181 ccgaagcccg agatcagcgc gaggatcatg tagggcatca ggctctctgc aaggccgatc
     3241 gagacgccga aataattgaa gacgagccgc gtgggctcgc tcgtgagccc gctccccatc
     3301 agcgccgcgt tgacgagacc gcgatcgccg agaagggcga gccagccgaa cacgcgcacc
     3361 acgcccgaga cgagcatcgg cgcgactgcg agcacggtga gcagcgcctt gaaacgcgag
     3421 cgcgtgcgga agagaaacaa ggcgagcgga taggcgagga agagagctag cgacgtcgag
     3481 acgagcgaca gccagatcga gttccaggtg agctcgagcg tgaagctgtc gccgagcgtc
     3541 gcgcgaaacg tgaccagcga ccaggcctcc accatggccc cggtcgggcc cgtctcgttg
     3601 aaggccatgc gaccgaggcc cgccatcggc acgacgtaga gcaagacgag aaccgccacg
     3661 gccggcacga cgagcaggac gccaatgagc cgcgcgagcg gagatcgctc gaggccgctc
     3721 atgtcgccgg caccgcaaag gcgagcgtat cctccggccg ccacgcgatc atgacctcgc
     3781 gtccgaccgg cggcgcgtcg gcggcgtgtg gcagctcgac gatgagacgc ccggcctcgg
     3841 tctcgacggc gagctcgatg cgatcgccga cgaacatgct cgccgcgatc cgccccgcaa
     3901 gaccgttgag cgcatcgggc agcgcatcgg cggcctcgcg cagcatcagg cgttgcgggc
     3961 ggaggaagat ctcgacagac ccaggcggcg gcgctttcgc aagccgcagt gctcgcccgg
     4021 caagcgcgag ccgaccactc gcgtccggcg cggcattcaa gacgttgctc cggccgacga
     4081 aatcggcgac gaagcgcgag gccggccggt cgtagatgtc gcgcgggctc gccacctgct
     4141 cgatccggcc ggcattcatg accgcgatcc ggtcgcacat cgtcagcgcc tcagtctggt
     4201 cgtgcgtgac gaacagcgtg gtgagagaga gcgcgcgctg cagatcgcgg atctcgagcc
     4261 gcatggcgtc gcggagtttt gcgtcgaggt tcgacaggct ctcgtcgagc agcagcacgt
     4321 caggctcgat gacgagcgcg cgggcgaggg cgacccgctg ctgctgcccg ccggaaagct
     4381 cgcgcggctt gcgggcggcg agg
//
LOCUS       DAORNH010000350         4377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_14936_length_4377_cov_2.539565, whole genome shotgun sequence.
ACCESSION   DAORNH010000350 DAORNH010000000
VERSION     DAORNH010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14936_length_4377_cov_2.539565"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>367)
                     /locus_tag="PK264_23045"
     CDS             complement(172..>367)
                     /locus_tag="PK264_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78778.1"
                     /translation="SSVRDELKLGGLVVMAGSPVATTVPVALVHRRKGYLNAAACALI
                     AILNEGTARRQPPARRRLGP"
     assembly_gap    368..396
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1019..1219
                     /locus_tag="PK264_23050"
                     /pseudo
     CDS             1019..1219
                     /locus_tag="PK264_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419417.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1358..2167)
                     /locus_tag="PK264_23055"
     CDS             complement(1358..2167)
                     /locus_tag="PK264_23055"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRD78779.1"
                     /translation="MLTRRQAAAASLVALSQMMTARSMAEKPAETYHDRMMKAADYPN
                     EQIALLVYPGFTALDMVGPQYAFGSMIGAKVHIVAKTMEIVESDTKLAFRPSATFGTC
                     PDPVDIIFVPGGTKGTLAAMEDKETIAFLKSRGEKAKLVTSVCTGSLVLGAAGLLKGY
                     RATSHWLTLDLLAEFGAVPDDRRVVIDRTRMTGAGVTAGIDFGLQIVEKLRGREYAEA
                     VQLLAEYDPDPPLKAGNPKTAPKAARDLLTEMFAPFREDVRQAAARIAMSR"
     assembly_gap    2243..2252
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2257..>2509)
                     /locus_tag="PK264_23060"
     CDS             complement(2257..>2509)
                     /locus_tag="PK264_23060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD78780.1"
                     /translation="KGLAIGAGVYAASRKAIGIDNLYFTPSFMSVDGKISYEIGSWTF
                     AVIGKNLLDAEYWQPYFYFDGRVAPSEPRTVFGSVSYKF"
     assembly_gap    2510..2622
                     /estimated_length=113
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2623..>4052)
                     /locus_tag="PK264_23065"
     CDS             complement(<2623..>4052)
                     /locus_tag="PK264_23065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRD78781.1"
                     /translation="YYFQGIGYRIRGFESEVLVDGFPLYYGGGDFSSLVNVERIEVLK
                     GPGGIFYQGGQTLTGGVVNLLSKLPHGERRYEVGVVFGGHGTWIPWFDINQPLNASKT
                     ALFRMTAEFEQSREFIDVVERERYSLNPTFMFRDDASTLVIQLHASRRTQHEYSGLPA
                     VGTIDRSSFSIRRDLFVGDPAIPKHVNANERIAAYFKHKLDDVWTIDVSARYGRSSLF
                     EPTQALFNFFGSSNQPVFGSNFSVLSALLTETTHEMAFNANAIAKFQIADSRNTFLVG
                     IDFNRTTDEGKLTGTFPYLVDFLNPTFPRYTFDQPLAFTIVDNTYWNSGLSIQLHSTI
                     KDRFHILLGARLAHVDVISRELTTGGSFHTDEWKVLPRAGIVVDIVRGVSAFASYSEG
                     LRAVPFFSAAAAPKPEESRQIEGGLKIALPTGLSATLALFEISRLNVATPDPNVPFAQ
                     VQTGEQQSRGFDIDLVWQPIAGLSVL"
     assembly_gap    4053..4062
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4063..4250)
                     /locus_tag="PK264_23070"
     CDS             complement(<4063..4250)
                     /locus_tag="PK264_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78782.1"
                     /translation="MTRTTYGATTGYRALTSVSATKTGAPLDQTPQSVAVVPRAVIDD
                     TAPLSVGEALKNVSAVSSG"
BASE COUNT          766 a         1318 c         1394 g          737 t
ORIGIN      
        1 gtgtaaccta tgtgtccgga acgaagtgca ccccctgtct cgggaaggtc atcaataact
       61 ttagatcgat tttcagctcc cgtgacggac agagattagg gctgcttgcc atctgctgac
      121 cacgatagcg ggaactttca ggaacatgcg gtttcgctcg ggcgaggcgg ttcatggccc
      181 aagacgtcgc ctagcgggtg gctgtcggcg ggccgttccc tcgttcagga ttgcgatcag
      241 cgcgcaggct gcagcgttga ggtagccctt gcggcggtgg accaaggcga cgggcacagt
      301 cgttgcgacc gggctacctg ccatcaccac aaggcctccg agcttaagct cgtcgcgcac
      361 gctgctcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaagc ccgcctcgac cagccgcctc
      421 tgcgccgtga ggctgtcgat gatcataacc ggcgcatcgt cgcggccggc ggcggcgagc
      481 tgacgcgcca gcatgtggcc gacgccccgc ggccccgcgc gggcggaaat ccgatccagc
      541 gctcacccac gagatcgcgc tgccccgcat ggcgtccttc gagctgatgc ccgggcgcgg
      601 cgatcaccac catgcgctct tgcgcaacga tggtggatag cagctcggcc ctcgcctctg
      661 ggcagtactt caggccctgc gtcgcctcac cgcagcgcac aaggtcggcc atctcctggc
      721 tgatcgtggt tctgagatcg agcgtggcgc ctttcgagtg ccgcgcgaaa cggcgcagca
      781 cgtcgaagat atgagtgtcg gcaagcgtgt cgacgagagc cagcgatgtg cgctggcgcg
      841 cgcccccatt ctccggccgc cgccgcgcac acggcgtcgc ggccgtcgtc gatggcagcc
      901 agcacggcct gcgcgaacgg caggaatgct tgtcctgcgg tcgaaagcca aaccgtggcc
      961 tgctcacgct cgagcagcgg cacgccgagc ttgtgctcga gcaggccgat gcggcgactg
     1021 gatcagcagc atcgggctcg atcgactgaa gtacggaacc cgctcgctgc gccggaccaa
     1081 agcagcgctg gctaccggcg aacgggaatc tcagagcggt gcaactttcg ctcggacaca
     1141 cgaaaatcga aagtactgtt cgttactttg gcgtcgaaat gacaatgcga ttgagatcgc
     1201 cgaaaaggtc gacatctgaa agcctcgggc agagccgaca tccgggcggc cgcctgtgac
     1261 cgtccgagca agctccgttg ctggatagac atggcagtct ggcccgccga gcgccgaacg
     1321 gctccggagc acattcggcc tgcgcctggc gagaccctca ccgggacatt gcgatgcgag
     1381 cggccgcctg cctcacgtcc tcgcggaagg gcgcgaacat ctcggtgaga aggtcgcgcg
     1441 cggccttcgg cgcggtcttg gggtttccgg ccttgagcgg cgggtcggga tcgtactcgg
     1501 caagcagctg aacggcctcg gcgtactcgc ggccgcgcag cttctcgacg atctgcaaac
     1561 caaaatcgat gcctgcggtg acgcccgcac cggtcatcct cgtccggtcg atcaccaccc
     1621 tgcgatcgtc cgggacagcc ccgaactccg ccaggagatc gagcgtcagc caatgcgagg
     1681 tcgccctgta gcccttcaga aggccggccg cgccgaggac gagcgagccc gtgcacacgc
     1741 tcgtcaccag cttcgccttc tccccgcgcg acttcaggaa ggcgatcgtc tccttgtcct
     1801 ccatcgcggc gagcgtgccc ttggtcccgc cggggacgaa gatgatgtcg acagggtcgg
     1861 ggcaggtgcc gaatgtcgcc gagggacgga atgcgagctt cgtgtcgctc tccacgatct
     1921 ccatcgtctt ggcgacgatg tggactttcg cgccgatcat ggacccgaag gcatactgcg
     1981 gcccaaccat gtcgagggca gtgaagccgg gatagaccag cagtgcgatc tgctcgttgg
     2041 ggtagtcggc ggctttcatc atccggtcat gataggtctc ggcaggcttc tcggccatcg
     2101 agcgcgccgt catcatttga gaaagtgcca cgagcgatgc cgcggcggcc tggcgacggg
     2161 tcagcatgct ggtcctcctg tcgtcaaaaa tcgtaacgac gcgggccgga cgttaccgtc
     2221 cgactcgcgc ctgcctcgct gcnnnnnnnn nngacgttag aacttgtagg acaccgagcc
     2281 gaaaaccgtc cgcggctcgc tcggcgcgac gcgtccatcg aaatagaagt agggttgcca
     2341 gtactcggca tcgagcaggt tcttgccgat cacggcgaac gtccacgaac cgatctcgta
     2401 gctgatcttg ccgtcgacgc tcatgaagct cggagtgaag taaaggttgt caatgccaat
     2461 ggctttccgg ctcgcggcgt agactcctgc gccgatggcc agtcccttgn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngaggaccg acaggcctgc
     2641 gatcggctgc cagactaggt cgatgtcgaa cccgcgcgac tgctgctcgc cggtctggac
     2701 ctgcgcgaac ggcacgttcg gatccggcgt cgccacgttg agccgtgaga tctcgaagag
     2761 ggcgagcgtc gcgctgagcc ccgtcggtag cgcgatctta aggccgcctt cgatctgccg
     2821 ggactcctca ggcttgggcg ccgcggccgc cgagaagaac ggcacagctc tcagcccctc
     2881 gctgtagctc gcgaaggccg agacgccccg tacgatgtcg acgacgatgc ctgcacgagg
     2941 cagcaccttc cattcgtccg tatggaaaga tccgccggtc gtcagctctc tcgagatgac
     3001 atcgacgtgg gccagacgcg ccccgagcag gatgtggaag cgatccttga tcgtcgagtg
     3061 cagctggatt gagagtcccg agttccagta ggtgttgtcg acaatcgtga acgccagcgg
     3121 ctggtcaaac gtgtaccggg gaaacgtcgg gttcaggaag tcaacgagat aggggaaggt
     3181 gcccgtgagc ttgccttcgt cagtggtccg attgaaatcg atgccgacga ggaaggtgtt
     3241 gcgactgtcc gcgatctgaa acttggcaat cgcgttggca ttgaacgcca tctcgtgtgt
     3301 cgtctcggta agcagggccg agagaaccga gaagttggag ccgaaaaccg gctggttgga
     3361 gctgccgaag aaattgaaga gagcctgcgt cggctcgaac agcgaggatc tgccgtatcg
     3421 cgcggacacg tcgatggtcc acacatcgtc gagtttgtgc ttgaagtagg cggcgatccg
     3481 ctcattggcg ttgacgtgct tcgggatcgc gggatcgccg acgaaaagat cgcgccggat
     3541 cgagaagctc gatcgatcta tggtgccgac ggcgggcagc cccgagtact cgtgctgagt
     3601 cctgcgggat gcatgcagct ggatcacgag tgtgctcgcg tcgtcccgga acatgaaagt
     3661 cggattgagt gaataccgct cgcgctcgac gacgtcgatg aactcgcgcg attgctcgaa
     3721 ctcggcagtc atgcggaaca gtgctgtctt cgaggcattg agcggctggt tgatgtcgaa
     3781 ccaagggatc caggtgccgt gccctccgaa caccacgccg acctcgtatc ggcgttcgcc
     3841 gtgaggcagc ttcgagagaa ggttgactac gccgccggtg agcgtttggc cgccctgata
     3901 gaatatgccg ccgggtccct tcaggacctc gatgcgctcc acgttgacca gcgagctgaa
     3961 gtcgccgcca ccgtaataga gcggaaagcc gtcgaccagg acctcggact cgaagccacg
     4021 tatgcggtag ccgataccct ggaaatagta agnnnnnnnn nnccggacga caccgccgag
     4081 acgttcttca gcgcctcccc gacagagagc ggcgccgtat cgtcgatgac ggcgcgcggg
     4141 acgacggcca cggattgcgg cgtctggtcg agtggagcgc cggtcttcgt cgccgagacg
     4201 ctcgtgagcg cgcgatagcc ggtggtcgcc ccgtaggtcg ttcgcgtcac gggggggccg
     4261 ccgtccacga cgacgcctgg cgggatggcc acgctgggag gaacggtgct gggaggagcg
     4321 gtgctggcga ccggcgcggc ccccggccag gactgagcgg cgcgggccac cgcctgg
//
LOCUS       DAORNH010000351         4341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15086_length_4341_cov_1.943537, whole genome shotgun sequence.
ACCESSION   DAORNH010000351 DAORNH010000000
VERSION     DAORNH010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15086_length_4341_cov_1.943537"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    209..345
                     /estimated_length=137
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            438..>790
                     /locus_tag="PK264_23075"
     CDS             438..>790
                     /locus_tag="PK264_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HRD78783.1"
                     /translation="MLTPIATTNAPNAVGPYSQAIKANGLVFCSGQVSLDPATMTIVE
                     GGIESWKRAGLPVMKDASQPLELQRQVQLIAGFLIVLGAVLSVSVSPVFVWLSAFVGA
                     GLMLAGATGWCGMAT"
     assembly_gap    791..845
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1042..1461
                     /locus_tag="PK264_23080"
     CDS             1042..1461
                     /locus_tag="PK264_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HRD78784.1"
                     /translation="MSKAPEGKRPMPEPTPETKHFWDGTKTGELRLQTCNDCTHTYFP
                     PRPFCPKCGSRGVKVVKASGKAKLYSYVIHHRPAQGFTPPYAIAVVELAEGPRLMTNI
                     VDCPQTPEALQLDMALEVTFAKQTDDITLPLFKPASK"
     gene            1475..2617
                     /locus_tag="PK264_23085"
     CDS             1475..2617
                     /locus_tag="PK264_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HRD78785.1"
                     /translation="MKPGSIAVVGAAETTDLGVIPGLSQIQLHADAALNAMKDCGLKP
                     SDIDGIATAGETPITLAHYLGITPTWADGTAVGGCSFMLHVRHAAAAISAGLCKTVLI
                     THGESGKSRVAVTPRPPYPGSLAGQFEAPYGVFGPPSMFTIPVLRYLKHYGISEEQLA
                     MVSVVQREWAAKNPRSTFKDPITVADVLKSRMIAYPFRILMCCLVTDGGGALILTSAE
                     RAKDFPTKPVYILGTGESVESPMISQMADFTSSRAFRVAGSKAFETSGIKHKDVDHLM
                     IYDAFAHLPFYGLEDLGFVKRGEAAAFIGERHSAPGGKLPINTNGGGLSYMHSGMYGM
                     YALQESVRQMRGIAPAQVKGAKISVCHGVGGMFAASGTIVFSNQKP"
     gene            complement(2681..>3591)
                     /gene="argC"
                     /locus_tag="PK264_23090"
     CDS             complement(2681..>3591)
                     /gene="argC"
                     /locus_tag="PK264_23090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRD78786.1"
                     /translation="TTGLEIRERLAASKTIEVRSIDPAKRKDPKARQALMGGVDVVIL
                     CLPDDAAKEAVALAGELGDKGPRIIDASTAHRVAEGWTYGFPEMTKGQAEAVAKARRI
                     ANPGCYPTGGIALIRPLVEAGIMPADFPVTVNAASGYSGGGKSMIEAYQAGTAPSFEL
                     YGLGFEHKHVPELQKYSMLKRRPIFIPSVGNWRQGMLVSVPLHLDTLPGKPKAADLEA
                     ALTAHYKGAELVRVVPAANAKGGRLGIEDLNKTDVMELSVFSHPGHGQAVLVARLDNL
                     GKGASGAAVQSLGLMLGVDVGICAAG"
     assembly_gap    3592..3734
                     /estimated_length=143
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3735..3930
                     /locus_tag="PK264_23095"
     CDS             <3735..3930
                     /locus_tag="PK264_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78787.1"
                     /translation="QKSRAVSAARRDRIKRPSSHPRKLARTATSQSEVGLDHASGMSD
                     TPTLVKIGTAVLLIGERGHC"
     assembly_gap    3951..4119
                     /estimated_length=169
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4120..>4341
                     /locus_tag="PK264_23100"
     CDS             <4120..>4341
                     /locus_tag="PK264_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78788.1"
                     /translation="GGGEAEASELTHLIGHRDAVKPQLTVAENLAFWQAYLTGSGSDP
                     AGIDMALAALGLEALADVPAGFLSQGQRRR"
BASE COUNT          677 a         1312 c         1225 g          623 t
ORIGIN      
        1 gttgcgctcc ggtcggcggc tgaaggcaga cgagccccaa gacgaccacg agaaaacgga
       61 tgaaggggcg acgcaacgac atggcacagg tacccgcaag ccggcccatc ttggcgaggt
      121 cacggccttg aaaagacaac gcaacggatc cgacgccgtg gggaaaaagg tagcagacgg
      181 gtcggccgac gtccgccaat cggccaggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctgg accggcatcc
      361 gccagagtcc gctctctggc gtacaatggc tttatcctgt cgtattcacc caatcaccat
      421 tcaccccatc atccactatg ctcacgccca tcgccaccac caacgccccc aacgccgtcg
      481 gaccttactc gcaggccatc aaggccaatg gcctcgtctt ctgctcgggc caggtctcgc
      541 tcgaccccgc cacgatgacc atcgtcgagg gcggcatcga gagctggaag cgcgccggtc
      601 tgccggtcat gaaggatgcg tcccagccgt tggagctgca acgccaggtg cagctgatcg
      661 cgggtttctt gatcgtgctc ggtgctgttc tcagcgtctc ggtttcgccg gtattcgtgt
      721 ggctgtcggc cttcgtcggc gccgggttga tgcttgccgg cgcgacaggc tggtgcggca
      781 tggcgacgat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnngacaa tcgagcacgc ggttcacggg aatgaccccg gacgccatcg cgaggatgac
      901 gaggtgaggg cggcggaggg caggcgcagc attttcaatg ccgatcgacg cggccaccac
      961 gcacgtcacg ctttcccata gacagaccgt gcgtttcctg gtgcgctgga tgccgagccc
     1021 accagcacgc acggagtgat catgagcaag gcccctgaag gcaaacggcc catgccggag
     1081 ccgaccccgg agaccaagca cttctgggac ggcacgaaga cgggcgagct tcggctgcag
     1141 acctgtaacg actgcacgca tacctatttc cccccacgcc ctttctgccc gaagtgcggg
     1201 tcgcgcggcg tcaaggtcgt gaaggcctcc ggcaaggcca agctctattc gtacgtgatc
     1261 catcaccgcc cggcccaagg cttcacgccg ccctatgcga tcgccgtggt cgaactcgcg
     1321 gaaggccccc gcctgatgac caacatcgtc gattgcccgc agacgccgga ggccctccag
     1381 ctcgacatgg ccctcgaggt gacattcgcc aagcagacgg acgacatcac gctgcccctg
     1441 ttcaagcccg cgtcgaagtg agaggagcgc acccatgaaa cccggctcca tcgccgtcgt
     1501 cggcgcggca gagaccaccg atctcggcgt catccccggc ctgtcgcaaa tccagctcca
     1561 tgcggacgcg gccctaaatg cgatgaagga ttgcggcctc aaaccctccg acatcgacgg
     1621 catcgccacc gccggcgaga cgccgatcac gctcgcccac tacctcggca tcacgccgac
     1681 ctgggccgac ggcaccgcgg tcggcggctg ctcgttcatg ctacatgtgc gccatgcggc
     1741 cgccgcaatc agcgcgggcc tctgcaagac cgtgctcatc acgcacggcg agagcggcaa
     1801 gtcgcgcgtc gccgtaacac cgcgcccgcc gtatcccggc agcctcgccg gccagttcga
     1861 ggcgccctac ggcgtgttcg gcccgccgag catgttcacg atccctgtgc tgcgctatct
     1921 gaagcactac ggcatcagcg aggagcagct cgccatggtg tcggtcgtgc agcgcgaatg
     1981 ggcagcgaag aacccgcgct cgaccttcaa ggacccgatc acggtcgcgg atgtcctcaa
     2041 gagccgcatg atcgcctacc cgttccgcat cctgatgtgc tgcctcgtca ccgacggcgg
     2101 cggtgcgctc attctgacga gcgccgagcg cgccaaggat ttcccgacca agcccgtcta
     2161 catcctcggc acgggcgaga gcgtcgaaag cccgatgatc agccagatgg cggatttcac
     2221 ctcgagccgc gccttccgcg tcgccggctc caaggccttc gagacgtcgg gcatcaagca
     2281 caaggacgtg gatcacctga tgatctacga cgccttcgcg catctgccgt tctacggcct
     2341 cgaggatctg ggcttcgtga agcgaggcga ggcggcggcc ttcatcggcg agcggcattc
     2401 ggcacccggc ggcaagctgc cgatcaatac gaacggtggc ggcctctcct acatgcactc
     2461 gggcatgtac ggcatgtacg ccctccagga gagtgtgcgc cagatgcgtg gcatcgcgcc
     2521 cgcgcaggtg aagggcgcca aaatctcggt ctgccatggc gtcggcggca tgttcgccgc
     2581 ctcgggcacg atcgtgtttt cgaaccagaa gccgtgacag acatggggtg ccaggccatc
     2641 tcggccgggc accccatcgt tacgggatca cgtcaccgga tcagcccgcc gcgcaaatgc
     2701 cgacatcgac gccgagcatc agcccgagcg actggacggc agcgcctgag gcgcccttgc
     2761 caagattgtc gagccgcgcg accagcacgg cctgcccgtg gcccggatgg gagaacacgg
     2821 agagttccat cacgtccgtc ttgttgaggt cctcgatgcc gagccgcccg cccttggcgt
     2881 tcgcggccgg gacgacgcgc acgagctccg cgcccttgta gtgcgcggtc agtgccgcct
     2941 cgaggtccgc ggccttgggc ttgcccggca gcgtgtcgag gtgcagcggc acgctgacga
     3001 gcatgccctg ccgccagttg ccgaccgaag gaatgaagat cggccggcgc ttcagcatcg
     3061 aatacttctg cagctcgggc acatgcttgt gctcgaagcc gagaccgtag agctcgaagc
     3121 tcggcgcggt gcctgcctga taggcctcga tcatcgactt gccgccgccg ctgtagcctg
     3181 acgccgcgtt gaccgtgaca gggaagtcgg caggcatgat gccggcttcg accaacgggc
     3241 ggatgagggc gatgccaccc gtcggatagc agcccggatt ggcgatccgc ctcgccttgg
     3301 cgacggcctc ggcctgaccc ttggtcattt cgggaaagcc gtaggtccag ccctcggcga
     3361 cgcggtgcgc cgtgctcgca tcgatgatcc tcgggccctt gtcgccaagc tcgccggcaa
     3421 gcgcaacggc ctccttggcg gcatcgtcgg ggaggcagag gatgacgacg tcgaccccgc
     3481 ccatcaacgc ctggcgcgcc ttcgggtcct tgcgcttggc tgggtcgatg ctcctcacct
     3541 cgatggtctt ggaagccgcg agccgctcgc ggatttcgag accggtggtg cnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnacaaaa aagccgcgcg gtcagcgcgg ctcgacggga tcggatcaaa
     3781 cggccctcat cgcatccgcg caagcttgca cgaacggcga cgtcgcagag cgaggttggc
     3841 cttgatcatg cgtctggtat gagcgacacg ccgacgcttg tcaagattgg cactgccgtg
     3901 ctgctgatcg gtgaacgtgg tcactgctga ggggaggagc cgttgcggct nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtggcggcg aggccgaagc
     4141 gagcgagctc acccacctga tcggccatcg cgacgccgtg aagccgcagt tgaccgtcgc
     4201 cgagaacctc gccttctggc aagcctatct gaccggcagc ggcagcgacc cggccggcat
     4261 cgatatggct ttggcggccc tcgggctcga agcgctcgct gatgtgccgg cgggcttcct
     4321 gagccagggc cagcgtcggc g
//
LOCUS       DAORNH010000352         4316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15184_length_4316_cov_2.298521, whole genome shotgun sequence.
ACCESSION   DAORNH010000352 DAORNH010000000
VERSION     DAORNH010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15184_length_4316_cov_2.298521"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>277
                     /locus_tag="PK264_23105"
     CDS             34..>277
                     /locus_tag="PK264_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HRD78789.1"
                     /translation="MSATEAFLEARKLLLAQRNDPDRAYAKFAWPQLETFNWALDWFD
                     VYAKTNTRPALWVASRDAGETKLSFRELSERSNQVAN"
     assembly_gap    278..392
                     /estimated_length=115
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <393..1365
                     /locus_tag="PK264_23110"
     CDS             <393..1365
                     /locus_tag="PK264_23110"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRD78790.1"
                     /translation="LTRDELLDRFERGDVRHVIVGAGDASKFADIRGDYTRIAVGNAP
                     SGWARFDDAYRAAPTYVPHGPTPARMAKAAAVLARFAEDRRRLLPRKAPAASTASAKL
                     PAGKVTLSEVESKAVLATYGVPVTKEIVVPEGRDVAAAAKAMTAPFAVKIVSRDIAHK
                     TEAGGVKLTVAAADLAKASAEIFANARRYKADAKIDGVLVAEMASGLEALIGVINDPG
                     FGPTVALGLGGILTEVLKDVTYRIAPFDIETARDMIAELRAAKLFDGYRGKPAADKEA
                     LAKMLVDVSRMAMALAPRLVEMDINPVFVDERGKGVVAADALIVLKE"
     gene            1371..1784
                     /locus_tag="PK264_23115"
     CDS             1371..1784
                     /locus_tag="PK264_23115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRD78791.1"
                     /translation="MAEEHVANLKSRDIPFARMLGIEIVSAEPDRVVARMLVRPEVCT
                     SFPSIHGGAVMAFADTLGACGTVLNLPPGASTTTMESKTNFIGPAKSGTYVTGEATPI
                     HRGRRTQVWQTRITNADGKLVAVVTQTQMVLEGRS"
     gene            1816..2406
                     /locus_tag="PK264_23120"
     CDS             1816..2406
                     /locus_tag="PK264_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78792.1"
                     /translation="MTTFATLGPAGTNHALVTRRYIEFHGLREARIALVATFDEAVAD
                     ILARRADFVVQCAVHPDTPRILGANYRRLFAIDSFISPSRELAILTRAEIVEPTSIGL
                     VMPATEGYADLSQWKVRVPERAIPIILEKLLAGQIDSGLVYLDYAERHPGRFRVEAEL
                     GSPDDVWIVYGRERAYRGELIACRHGLAASRYAGRE"
     gene            complement(2464..3012)
                     /locus_tag="PK264_23125"
     CDS             complement(2464..3012)
                     /locus_tag="PK264_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HRD78793.1"
                     /translation="MDGYKFNKFAGGALSALLFIFGAKTLVEITQHSSHASSKLGIEL
                     PAAAKGATATAAPAAAKYEFAKLKPLLEKASVEEGEVVFKPCVSCHTVEKGGPNRVGP
                     NLYGIIGRKVASVGGFEYSEPMKKKGGDWTFDTLGAFLYDPKGTVPDTKMVFNGVQDM
                     KDLADLIGYLRKHADAPAELPK"
     gene            3199..>3895
                     /locus_tag="PK264_23130"
     CDS             3199..>3895
                     /locus_tag="PK264_23130"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRD78794.1"
                     /translation="MPRALILIPARMQASRLPGKPMADIAGEPMIVHVWRRAMEAEIG
                     RVVVATDATEISNAIRNAGGEAVMTRSDHMTGSDRIFEALNHVDPDGDFDTIVNVQGD
                     LPTLDPKLVRACIEPLAVKGPHIATIACAIMVAEERTNPNVVKVVGTPIAPNRLRALY
                     FTRATAPTGEGPLYHHIGMYAYRRQSLERFVALKPAPLETRERLEQLRALEDGMRIDV
                     AIVDTVPLGVDTPA"
     assembly_gap    3896..3936
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3953..>4316
                     /locus_tag="PK264_23135"
     CDS             3953..>4316
                     /locus_tag="PK264_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRD78795.1"
                     /translation="MPKRTSKTGAADSAGNRKPRIAYQGEPGANSHIACNEVAPDLEP
                     LPCPTFEDAIGAVKSGEARLAMIPIENSLAGRVADVHHLLPSSKLFIVGEHFLPIRFQ
                     LMALKGAKLSEITDVYSHV"
BASE COUNT          729 a         1407 c         1342 g          682 t
ORIGIN      
        1 cagcctgccc gagcgggttt cgaggagacg ttcatgagtg ccacggaagc gttcttagaa
       61 gcgcgaaaat tgctgctggc ccaacgcaat gatccagacc gcgcctacgc caagttcgcg
      121 tggccgcagc ttgagacgtt caattgggcg ttggactggt tcgatgtcta cgccaagacc
      181 aatacccggc cagcgctttg ggtggcaagc cgcgatgcgg gcgagaccaa gctctccttc
      241 cgcgaactct cggagcggtc gaatcaggtc gccaactnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngctgacac gcgacgagtt actcgaccgc
      421 ttcgagcgcg gcgacgtccg tcatgtgatc gtcggtgcgg gggacgccag caagtttgcc
      481 gacattcgcg gcgactacac gcgcatcgcc gtcggcaacg caccttccgg atgggcgcgc
      541 ttcgacgatg cctatcgggc ggcacccacc tatgttccgc acgggccgac gccggcgcgc
      601 atggccaagg ccgcggccgt gctcgcgcgc tttgccgagg atcgccgcag gctcctgccg
      661 cgcaaggccc ctgcagcttc gaccgccagc gccaagttgc cggccggaaa agtgacgctc
      721 tccgaggtcg agagcaaggc ggtgctggcg acttacggcg tgcccgtgac gaaagagatc
      781 gtggtgcctg aaggccgcga cgtcgcggca gccgccaagg ccatgacggc gccgttcgcg
      841 gtgaagatcg tctcgcgcga catcgcccac aagacagagg ccggcggcgt aaagctcact
      901 gtcgcggcgg ccgacctcgc caaggcctcg gccgagatct tcgccaacgc gcgccgctac
      961 aaggccgatg ccaagatcga cggagtgctc gtcgccgaga tggcttcggg tctcgaagcg
     1021 ctgatcggcg tcatcaacga tccgggcttc ggaccgacgg tggcgctcgg cctcggcggc
     1081 atcctgaccg aggtcctgaa ggacgtgacc taccgcatcg cgcccttcga catcgagacg
     1141 gcacgcgaca tgatcgcaga gctccgcgca gccaaacttt tcgacggcta tcgcggcaag
     1201 ccggccgcgg acaaggaggc gctcgcgaag atgctggtcg atgtctcgcg catggcgatg
     1261 gcactggcgc cccgactcgt cgagatggac atcaaccccg tgttcgtcga cgagaggggt
     1321 aagggcgtcg ttgccgccga cgcgctgatc gtgctcaagg agtagggccc atggccgaag
     1381 agcacgtcgc aaacttgaag tcgcgcgaca ttccctttgc gaggatgctc ggcatcgaga
     1441 tcgtctcggc ggagcccgat cgcgtcgtcg cgcgcatgct ggtgcggccc gaggtgtgca
     1501 cgagcttccc gtcgatccac ggcggcgccg tcatggcctt tgccgacacg ctcggcgcct
     1561 gcggcacggt gctcaatctg ccgcccggcg cctcgacgac gacgatggaa agcaagacga
     1621 acttcatcgg tcccgcgaag agcggcacct acgttaccgg cgaggcgacg ccgatccacc
     1681 gcgggcggcg cacgcaggtc tggcagacgc gcatcaccaa cgcggacggc aagctcgtcg
     1741 ccgtcgtcac gcagacgcag atggtgctcg aggggcggtc gtagacgtgg ccgacgcggc
     1801 cgaggcggaa cgacgatgac cacattcgcg acgctcggac cggcaggaac caaccacgcg
     1861 ctcgttacgc gccgctacat cgagttccat ggcctccgcg aagcccgcat cgcgcttgtc
     1921 gccacgttcg acgaggcggt cgcggacatc ctcgcgagga gggcggactt cgtcgtgcag
     1981 tgtgccgtac atccggatac accacgcatc ctcggcgcga actatcgccg cctttttgcg
     2041 atcgacagtt tcatctcgcc gagccgggag ctcgcgatcc tgacgcgggc ggagatcgtg
     2101 gagccgacct cgatcggtct cgtgatgccg gcgacggagg gctatgcgga tctctcgcaa
     2161 tggaaagtgc gcgtgccgga acgcgccatt ccgatcatcc tggagaagct gctcgcaggg
     2221 cagatcgatt cggggctcgt gtatctcgac tacgccgaac gccatcccgg ccgatttcgc
     2281 gtcgaggccg aactcggcag ccctgatgac gtatggatcg tctacgggcg cgagcgcgcc
     2341 tatcgaggcg aactgatcgc ctgccggcac ggcttggcag cctcgcgata cgcgggacgg
     2401 gagtgaggcg cttgatgttg ttcgcgcgtt ggccgcgtga aacagctcac gcggctcgca
     2461 ctcttacttc ggcagctcgg ccggcgcgtc ggcgtgcttg cgcaggtagc cgatgagatc
     2521 ggccaaatcc ttcatgtcct ggacgccatt gaacaccatc ttggtgtcgg ggacggtgcc
     2581 cttcggatcg tagaggaagg cgccgagtgt atcgaacgtc cagtcgccac cctttttctt
     2641 catgggctcc gagtattcga agccgcccac ggacgccacc ttgcggccga tgatgccata
     2701 gagattcggg ccgacgcggt tgggcccacc cttctcgaca gtgtggcagg agacgcacgg
     2761 cttgaagacg acttcgcctt cctcgacgct cgccttctcg agcagcggct tcaacttggc
     2821 gaactcgtat ttggcagcgg cgggcgcggc cgtcgccgtt gcgcccttgg ctgcggctgg
     2881 aagctcgatc ccaagcttgg aggaggcatg gctgctgtgc tgcgtgatct cgaccagcgt
     2941 cttggcgccg aagatgaaca gcagcgccga caatgcgccg ccagcgaact tgttgaattt
     3001 gtagccgtcc atcggtcgtc cggtccctgt gatccgccgc gaggtgtgtt cggcaacgcg
     3061 cccatcggcg cacgtccaac agcgcaccgg gccgcactat agaagccgcc gaaagccttg
     3121 caacagaact cacggctgtt ctaggaggtg cagccgagac caattgtcac ttcgaacaca
     3181 tccgagcagg cgagcccgat gccccgcgcc ctgatcctga tccctgcgcg catgcaggcg
     3241 agccggctcc ccggcaagcc catggccgac atcgccggcg agccgatgat cgtccatgtc
     3301 tggcggcggg ccatggaggc ggagatcggt cgggtcgtcg tcgcaaccga cgcgaccgaa
     3361 atcagcaacg ccatccggaa cgcgggcggc gaggccgtca tgacccgatc cgaccacatg
     3421 accggctcgg accgcatctt cgaggcgctc aatcacgtcg accccgacgg cgacttcgat
     3481 acgatcgtca acgtgcaggg cgacctgccg acgctcgatc cgaagctcgt gcgcgcctgc
     3541 atcgagccgc tcgccgtgaa gggcccgcac atcgcgacga tcgcctgcgc gatcatggtt
     3601 gccgaggagc gcacgaaccc gaatgtcgtg aaggtcgtcg gcacgccgat cgctcccaat
     3661 cgcctccgtg cgctctactt cacgcgcgcg acggcaccga cgggcgaagg gccgctctat
     3721 caccacatcg gcatgtacgc gtaccgccgc cagtcgctcg agcgcttcgt tgcgctgaag
     3781 cccgccccac tcgagacgcg cgagcggctc gagcagttgc gcgcgctcga ggacggcatg
     3841 cgcatcgacg tggccatcgt tgacacggtt ccgctcggtg tcgatactcc cgccgnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg aaccgatccc ccatgccgaa
     3961 acgcacttcg aagaccggcg ccgccgactc cgccggcaac aggaagccgc gcattgccta
     4021 tcagggcgag cccggcgcca actcccacat cgcctgcaac gaggtcgccc cggacctcga
     4081 gcctctcccc tgcccgacgt tcgaggatgc gatcggcgcg gtgaagtcgg gcgaggctcg
     4141 tctcgccatg atcccgatcg agaactcgct cgcgggccgc gttgccgacg tgcaccacct
     4201 gctgccgagc tcgaagctgt tcattgtcgg cgagcacttc ctgccgatcc gcttccagct
     4261 gatggccttg aagggcgcca agctctccga gatcaccgac gtctacagcc acgtgc
//
LOCUS       DAORNH010000353         4261 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15446_length_4261_cov_2.753685, whole genome shotgun sequence.
ACCESSION   DAORNH010000353 DAORNH010000000
VERSION     DAORNH010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15446_length_4261_cov_2.753685"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PK264_23140"
     CDS             complement(<1..541)
                     /locus_tag="PK264_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78796.1"
                     /translation="MRPTLLAMERLAISSWPARETRDVSGWLWRSSSGGSIRANSVST
                     LAYTGRDIDAAIAECERLYRDRGEPVRFNISEICAPVDLDARLTARGYGLSDPCTTLS
                     KPIASPSQPPGDVEVTAAPTSEWLEVYLAAISASRRPVAQSIVERVPGPRAFFACRRS
                     GRVIATGLSVADGPLAVVMC"
     gene            complement(565..>887)
                     /locus_tag="PK264_23145"
     CDS             complement(565..>887)
                     /locus_tag="PK264_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78797.1"
                     /translation="GPGAASGAVSFSHEIIADYLAARAAVRAISLQPDNPVALGQAIG
                     LRADLEPITRSVYLRTLVHALSRDRSLAAAVLETAQSSRIARIYKANVDILARELLRG
                     VKAA"
     assembly_gap    888..1027
                     /estimated_length=140
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1028..2495)
                     /locus_tag="PK264_23150"
     CDS             complement(<1028..2495)
                     /locus_tag="PK264_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78798.1"
                     /translation="MSELMSETRGFLVADGFAVGDAGAGLLLANRTSCGKLEERRVWV
                     GGAGAAPWTAERIAASFATLPAVGAATSSFLLVPETGGLSRDMLAVLKAHGIQVRVPV
                     QFFDTPYKWEPAEFGTQSGADAASVFHEFAKGSERLMSSRVAQPFHALSGLGEQRGGF
                     QSGPDLVEHLVEELTTPAVRPRLIVVAGAAGAGKSVAFAALFARLHAHFLGEKRHHRS
                     AGRPVLFLPQHIQEQQVSSLAGLLSAVASTDAAQPTSPDLMRWLNAAGRTIWMFDGLD
                     EFFSGESDFLPAIEEQIAPERRGRIVICTRDSLLTSSSGLAALLDRHVRTAGQGTVAI
                     YEVDRWQRPSQRALAYQRLEGRAPVRLEAADPPKVAAFLKHLDASPAAAELATLPFYC
                     SLMLGAAAQRGSGIAPLTEQDLLAEAVDGMIDREAEKLSGGEAGFAWDVFSGAETFAD
                     LAEIVEKMGHERFSNVLERGHIHAVLRQIGRDRLVELVE"
     gene            2665..>4261
                     /locus_tag="PK264_23155"
     CDS             2665..>4261
                     /locus_tag="PK264_23155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78799.1"
                     /translation="MTAAARSATPSPFERHRLAVSSADRVLQAAKGGVRQIVAELGID
                     GAQHAAHGYAWLATTVEALRQMVGWAERISASGHFGAIEQNILDIAFTEYLAQIAGGI
                     PMSQSEIVRLADLGVGADEARRFADGAAAAFLGADLTKAKAALARAIAADPSATTFGE
                     TGLDETLAAMRTEMRRFSVAEVLPHAHGWHTANEYIPLDVIAKMSEMGVFGLTLPESF
                     GGLGLGKEAMCVVSEELSRGYIGVGSLGTRSEIAAELILNNGTEEQKQKYLPRIASGE
                     ILPTAVFTEPNTGSDLASLRTRAERHGDVYRIYGQKTWITHPVRADVMTLLVRTNPKE
                     PGYKGLSMLLAEKPRGTDENPFPAKGMTGGEIEVLGYRGMKEFDISFDGFEVPTGQLL
                     GGSEGQGFKQLMNTFEAARIQTAARAVGVAQSAMDLALAYALDRKQFGKEIFSFPRVA
                     NKIVMMAVETMVARQITYFAAREKDQGRRCDLEAGMAKLLAARIAWANADSALQIHGG
                     NGFALEYPVSRVLCDARILNIFEG"
BASE COUNT          715 a         1389 c         1397 g          620 t
ORIGIN      
        1 tgcacatgac gacggcaagc ggaccgtcgg cgacggagag gcctgtcgcg atgacgcgtc
       61 cggaccggcg gcaggcgaaa aaggcccgcg ggcccggtac gcgctcgacg atcgactggg
      121 cgacgggccg ccggctcgcg ctgatcgcgg cgaggtagac ctcgagccac tcagatgtcg
      181 gggccgctgt cacctcgacg tcgcctggag gctgtgatgg cgatgcgatc ggcttcgaca
      241 gtgtcgtgca gggatcgctc aacccatagc cgcgggcggt gagcctcgcg tcgaggtcga
      301 ccggcgcgca gatctcggag atattgaagc ggacgggctc gccgcggtcc cgatagagcc
      361 gctcgcattc ggcgatggca gcgtcgatgt cgcggccggt gtacgccaga gtcgagacgg
      421 agttggcgcg gatcgagccg ccactcgacg agcgccagag ccagccggaa acatcacgtg
      481 tctcgcgagc gggccaggag ctgatcgcca gccgctccat ggcgaggagc gtggggcgca
      541 tggtcgctga caacgatcga ggcatcaggc cgccttcacg ccgcgcagaa gctcgcgagc
      601 cagaatgtcg acgttagcct tgtagatgcg cgcgatccgg ctcgattgcg ccgtctcgag
      661 aacggcagcg gcgaggctgc ggtcccggct caaggcgtgc accagcgtcc gcaggtagac
      721 cgatcgcgtg atcggctcga gatcggctcg gagcccgatc gcctggccga gcgcgaccgg
      781 attgtccggc tgcaggctga tcgcgcggac tgcggcccgt gcagcgaggt aatccgcgat
      841 gatttcgtgg ctgaagctca cggcgccact cgcggcgccc ggcccgannn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnncct cgacgagttc aacgaggcgg tcgcgaccga tctggcgcag cacggcgtgg
     1081 atgtggccac gctcgagcac gttcgagaag cgctcgtgcc ccatcttctc gacgatctcg
     1141 gcgagatcgg caaatgtctc cgcgcctgag aaaacgtccc aggcaaagcc cgcctcgccg
     1201 ccggagagct tctccgcctc gcgatcgatc atgccgtcga cggcctcggc taggaggtcc
     1261 tgctccgtca acggggcgat tcccgagccg cgttgagccg cggcgccgag catcaggctg
     1321 cagtagaagg gcagcgtcgc gagctcggcc gccgccgggc ttgcgtcgag gtgcttgagg
     1381 aatgcggcca ccttcggcgg gtccgccgct tcgaggcgca ccggggcgcg tccttcgaga
     1441 cgctggtagg cgagcgcgcg ctgcgatgga cgctgccagc ggtccacctc gtaaatcgcg
     1501 acggtgccct ggccggccgt ccgcacatgc cgatctagaa gggctgcgag gcccgaggac
     1561 gacgtgagca gactgtcgcg ggtacagatg acgatccgtc cgcggcgctc gggtgcgatc
     1621 tgctcctcga tcgccggcag gaaatcgctc tcgccggaga agaactcatc gagcccgtcg
     1681 aacatccaga tcgtgcggcc ggccgcattc agccagcgca tgaggtccgg cgatgtcggc
     1741 tgcgccgcgt cagtgctcgc gacggccgac aggaggcctg cgagtgagga gacctgctgc
     1801 tcctggatat gctgcggcag gaacagcacc ggacggccgg cactgcgatg atgccgcttc
     1861 tcgccgagaa agtgcgcgtg aagtcgggca aagagcgctg cgaaggcgac cgatttgccg
     1921 gcaccggccg ccccggcgac gacgatcaag cgaggccgga cggccggtgt cgtcagctct
     1981 tcaactagat gctcgacgag gtcgggccca ctctggaagc cgccgcgctg ctcgccgagg
     2041 cccgacaggg cgtgaaaggg ctgtgcgacc cggctggaca tgagccgctc gctgcccttc
     2101 gcgaattcat ggaacaccga tgccgcatcc gcaccggact gcgtgccgaa ctcggccggc
     2161 tcccacttgt agggtgtgtc gaagaactgg accggcactc ggacctgaat gccgtgcgcc
     2221 ttgagcacgg cgagcatgtc gcgcgagagg cccccggtct cgggcacgag gaggaatgaa
     2281 ctcgtcgccg cgcccacggc cggcaatgtc gcgaaactcg ccgcgatgcg ctcggccgtc
     2341 cacggggctg cgcctgcgcc gccgacccag acccgccgct cctcaagctt gccgcaagac
     2401 gtccggttgg cgaggaggag gccggcgcca gcatcaccga cggcaaagcc atcggcgacg
     2461 agaaagccgc gcgtctcgct catcagctca gacatgtccc ctcgcctcca tgcggagccc
     2521 agtgtaggtc gcatcttgag cgagataaag tgtcgacccg ccatctcagt gaggtgcggc
     2581 atcgaccgta gttgaattgc gctccggcga cactatggtg caccgcaata cgcaccaatt
     2641 cacgagcgcg acgaggatca cgccatgacc gctgctgcac gctccgcgac gccgtctcct
     2701 ttcgagcggc atcgtctcgc agtctcatcg gccgatcggg ttttgcaagc cgcgaagggg
     2761 ggcgtcagac agatcgtcgc cgagctcggc atcgacggcg cccaacacgc agcccacggc
     2821 tatgcctggc ttgcgacaac ggtcgaggcg ctgcgccaga tggtcggctg ggccgagcgc
     2881 atctccgcat cgggccattt cggcgcgatc gagcagaaca tcctcgacat agcattcacg
     2941 gagtacctcg cccagatcgc cggcggcatc ccgatgagcc agagcgagat cgtccgcctc
     3001 gccgatctcg gtgtcggcgc cgacgaggcg cgccgcttcg ccgatggcgc ggccgcggcc
     3061 tttctcggtg cagacctcac caaggccaag gcggcgctcg cccgcgccat cgccgctgat
     3121 ccaagtgcga cgaccttcgg cgagaccggc ctcgacgaga cgctcgccgc catgcgcacc
     3181 gagatgcgcc gcttctcggt cgccgaggtg ctgcctcatg cccacggctg gcacacggcg
     3241 aacgagtaca ttcccctcga tgtgatcgcc aagatgagcg agatgggcgt gttcggcctg
     3301 acgctgcctg agagcttcgg cggcctcggc ctcggcaagg aagccatgtg cgtcgtctcg
     3361 gaggagctct cgcgcggcta catcggtgtc ggctcgctcg gcacgcgcag cgagatcgcc
     3421 gccgagctca tcctcaacaa cggcaccgag gagcaaaagc aaaagtacct gccgaggatc
     3481 gcctcgggcg agatcctgcc gacggccgtc ttcaccgagc ccaacacagg ctccgacctc
     3541 gcctcgctcc ggacacgcgc agagcgtcac ggcgatgtct atcgcatcta cggccagaag
     3601 acctggatca cgcatcccgt gcgcgccgac gtgatgacgc tgctcgtgcg caccaatccg
     3661 aaggagccgg gctacaaggg cctctcgatg ctgctcgccg agaagccgcg cggcacggac
     3721 gaaaacccgt tcccggcgaa gggcatgacg ggcggcgaga tcgaggtgct cggctatcgc
     3781 ggcatgaagg agttcgacat ctccttcgac ggcttcgagg tgccgacagg tcagctgctc
     3841 ggcggcagcg aagggcaggg cttcaagcag ctcatgaaca ctttcgaggc ggcgcgcatc
     3901 cagacggcgg cgcgtgcggt cggtgtcgcc cagtcggcga tggacctggc acttgcctac
     3961 gcgctcgacc ggaagcagtt tggcaaggag atcttctcct tcccgcgcgt tgcaaacaaa
     4021 atcgtcatga tggcggtcga gaccatggtc gcgcgccaga tcacctattt cgccgcccgc
     4081 gagaaggacc agggccggcg ctgcgatctc gaggcaggca tggcgaagct gctcgccgcc
     4141 cgcatcgcct gggccaacgc cgacagcgcg ctgcagattc acggcggcaa cggattcgct
     4201 ctcgagtatc ccgtcagtcg cgtgctctgc gacgcgcgca tcctcaacat cttcgagggt
     4261 g
//
LOCUS       DAORNH010000354         4246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15520_length_4246_cov_2.344071, whole genome shotgun sequence.
ACCESSION   DAORNH010000354 DAORNH010000000
VERSION     DAORNH010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15520_length_4246_cov_2.344071"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..711
                     /gene="msrA"
                     /locus_tag="PK264_23160"
     CDS             43..711
                     /gene="msrA"
                     /locus_tag="PK264_23160"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HRD78800.1"
                     /translation="MLLFRKKSAMPTPSEALKGRVSAIPTASTHYVNGASLKGPYPAG
                     SEMAMFGLGCFWGAERKFWQLGHGVIVTAVGYAAGHTPNPTYEEVCSGMTGHNEVVLV
                     VFNPAETSYETLLKLFWESHNPTQGMRQGNDVGTQYRSGIYTFSEAQQVAAEASRKAY
                     QGALDAKRFGTITTEIKPAGEFYFAEDYHQQYLAKVPHGYCGLGGTGVTCPIGTGVAA
                     KPTG"
     gene            complement(714..1310)
                     /locus_tag="PK264_23165"
     CDS             complement(714..1310)
                     /locus_tag="PK264_23165"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRD78801.1"
                     /translation="MMLSDATRDRILAHGLGIASRAGWHALSFGDLARDLDLPKSGLA
                     RHFATKDELQLAVLDQAAQLFIRDVNDVAAGESQGATRLRTLFWRWLNWSRAPRLKGG
                     CPFVHASAESEALPPAVRARLKEVLDAWSAVLREAIDAARGQTIATDTDADQLIFELH
                     GLYLSHHFWHWSMRDPTAEARTMRAFDRIVSSPSRTGR"
     gene            1484..2422
                     /locus_tag="PK264_23170"
     CDS             1484..2422
                     /locus_tag="PK264_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HRD78802.1"
                     /translation="MAAIDREQILATAKLIEPHVRRTPVLSIDARELGLPSAPLVLKL
                     ESLQHTGSFKARGAFASLLSRGVPAAAVAAASGGNHGAAVAYAARELGHKATIFVPAI
                     SSPAKIARIRSYGADLVVGGERYVDALAACEAHIAATGAASVHAYDEVETLLGQGTLG
                     AELDAQAAGIDTLLVAVGGGGLIGGIAAWYDKRVRVIGVEPELAPTLAMALAAGRPVD
                     APGGGIAADSLAPRQVGELMFPIARRAVERVVLVTDAAIRDAQLRLWDTLRLVAEPGG
                     ATALAALLSGRYRPIEGERVAVLVCGGNTTAVDFDR"
     gene            complement(3204..>4007)
                     /locus_tag="PK264_23175"
     CDS             complement(3204..>4007)
                     /locus_tag="PK264_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78803.1"
                     /translation="ATRTIGIEPIHGTAIGLIRAVASVEPIVPVAAVTAISPIPAIET
                     VVPVLAIVSVEAGLAITEAAVPTIVMALPAMIIARATTVVAARAVVGAWLLTAAGPVV
                     APPLRSAWSVGLAAEARGGRLRLTDRHIGHPEARVHRRGVIIARVVVIMIERLTRQSR
                     PHAVAVRAVAMLRRLLLAHREDDSVVVLGVLQIVLSEHRIAGTQRIARERHVLFGNQG
                     RGAADLDVRAVAFEIPGERIVALAVVVVPTAATPVLLSLPHGLSSSLHG"
     assembly_gap    4008..4029
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4030..>4246)
                     /locus_tag="PK264_23180"
     CDS             complement(<4030..>4246)
                     /locus_tag="PK264_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78804.1"
                     /translation="RNWLLPLDSGLTLASDFPVHPVPLRPGPILGRAIGREPVRCRPI
                     WVSPFRTQPFPAMPITAMPITAAPVTA"
BASE COUNT          756 a         1318 c         1469 g          681 t
ORIGIN      
        1 caagagagat cgagtgaacc cgcgacccga acgaggtacg ccatgctgct gttccgcaag
       61 aaatccgcca tgccgacgcc gagcgaggcc ctaaagggcc gtgtgagcgc catcccgacg
      121 gcgtcgaccc actacgtgaa cggtgcgtcg ctcaaaggcc cgtatccggc cggcagcgag
      181 atggccatgt ttggcctcgg ctgcttctgg ggggccgagc gcaagttctg gcagctcggt
      241 cacggcgtca tcgtgacggc cgtcggctat gctgcgggcc acacgccgaa cccgacatac
      301 gaggaggtct gctccggcat gacagggcac aacgaggtgg tgctcgtcgt gttcaatccg
      361 gccgaaacgt cctacgagac gcttctcaag ctcttctggg agagccacaa ccccacgcag
      421 ggcatgcggc aggggaacga cgtcggtacg cagtaccgct ccggcatcta cacgttctcg
      481 gaggcacagc aagtcgccgc cgaggcgtca cgcaaggcct accagggcgc gctcgatgca
      541 aagcgcttcg gcacgatcac gactgagatc aagccggcgg gcgaattcta cttcgcggag
      601 gactatcacc agcagtacct cgcgaaggtg ccgcacggct attgcggcct cggcggcacg
      661 ggcgtcacct gcccgatcgg caccggcgtc gcagcgaagc ctaccggctg agctcagcgg
      721 ccggtacgcg aggggctcga cacgatgcgg tcgaaggcgc gcatcgtgcg agcctcggca
      781 gtcgggtcgc gcatcgacca gtgccagaag tggtgcgaga gatagagccc gtggagctcg
      841 aagatcagct gatcggcatc ggtgtcggtg gcaatcgtct ggccgcgtgc ggcatcgatc
      901 gcttcgcgga gcacagccga ccaggcgtcg agcacctcct tcaggcgcgc gcggaccgcc
      961 ggcggcaacg cctctgattc agcgctcgca tggacgaacg ggcaaccgcc cttgaggcgc
     1021 ggtgcgcgcg accagttgag ccagcgccag aacagcgtcc gcagtcgcgt ggcgccttgg
     1081 gactcgccgg cggcgacgtc gttcacatca cggatgaaga gctgcgctgc ctgatcgagc
     1141 acggcaagct ggagttcgtc cttggtcgcg aaatggcggg caaggccgct tttcggcaga
     1201 tcaaggtcgc gcgcgaggtc tccgaacgac aacgcatgcc agccggcgcg actcgcgata
     1261 ccgagcccgt gagctagaat tcgatcgcgc gttgcatccg acagcatcat gcacctcgtc
     1321 cctctggttt cgactagcga cggcgcgcca tctactttga tgcgcagtga ataagggcga
     1381 tcgtggtgag actgcggcat gactcttcac gctgacgtgt gtgctgacat gcgcagtgtc
     1441 gcatgcgaca cacgggccgc agccgacaac gatggagaca gccgtggcag cgatcgatcg
     1501 cgagcagatt cttgcgaccg cgaagctcat cgagccccat gtccgcagga cgccggtgct
     1561 gagcattgat gctcgcgagc tcggcttgcc gtcggcaccg ctcgtcctga agctcgagag
     1621 cctgcagcat acgggatcat tcaaggcgcg gggcgccttc gcgagcctcc tctcgcgtgg
     1681 ggtgccagcg gcggcggtcg cggcagcgtc cggcggcaat catggcgccg ctgtcgctta
     1741 cgccgcgcgc gagctcggtc acaaggcgac gatcttcgtc cccgccattt catcgcccgc
     1801 caagatcgcg cggatcagga gttatggcgc cgatctggtc gtcggcggcg agcgctatgt
     1861 ggacgcgctg gcggcctgcg aggcccatat cgcggcgaca ggggccgcgt ccgtgcatgc
     1921 ctacgatgag gtcgagacac tcctcggaca gggcactctc ggcgccgagc tcgatgcgca
     1981 ggcggcaggt atcgacacac tcctcgtcgc ggtcggcggc ggcgggctga tcggcggcat
     2041 cgcagcctgg tacgacaagc gcgtgcgcgt catcggcgtc gagccggaat tggcgccgac
     2101 cctggcgatg gctctcgccg cgggccgccc tgtcgatgcc ccgggaggcg gaatcgccgc
     2161 cgattcgctc gctccgcggc aggtcggcga actgatgttc ccgattgcca ggcgcgccgt
     2221 cgagcgggtg gtgctggtca cggacgcggc aatccgcgat gcgcaactca ggctctggga
     2281 tacgctgcgt ttggtggccg agcccggcgg cgcgaccgcc ctggccgcac tgctgagcgg
     2341 cagatacagg cctatcgagg gggagcgggt cgcggtgctg gtttgtggcg gcaacacaac
     2401 cgccgtcgat ttcgataggt gagagaaggt ttgggcaggg tgaccagacc cgcgcattgc
     2461 agtgtcccct ggacaaattg tgcttggaat cgcgtgaaag ccgagctagg gtacaagagc
     2521 gtaggtgggg tgagggcgct aggcttcgag gcttggcgat aaatccccgc agcctgcgtg
     2581 caggccattt gcgttgtgga atcgtgcttc gggcaccctc aagtttcggg cagcttgtag
     2641 cgaccaccgc ggtccgtggc cagccaaggc aaatcggcca ttcttgctcg gccattggtg
     2701 ctcgagtgaa ggtcctggag accgaatatc ctcctgcgca tcgcgcgggg gtcggacgtc
     2761 acctgatggg ccgctgctga aagagcgtgg gatcctttac aacgcagaca tcctagtccc
     2821 gggagcagct gtcgagtgtc ctgtgagagg atctcgtgga tgacaggcgg cgagcgcggt
     2881 agtcgcgtac ggcatctgcc tgcatgcggt cgaggccttg ggatttctga agcctcacgg
     2941 cgcgctgagc cgctcgaaag ctcggccgcg caaggctcga gaggggcagg ggcgatgatg
     3001 atgacgccgg cggcgcatcg gtcgataagt ttggggtcgg cgggatgtgc gtgccgatga
     3061 atggtttagc tgatcgactt cgagagtact cggagctgtt cgggatgcac gctttgcagg
     3121 ctcatgattg ctaggatctc attgaagcca gcgatgtaac gacggcagcg ggacgacttg
     3181 tcgtttggca ggcgtccgaa gaatcatccg tggagtgaag acgaaagccc atgaggcagg
     3241 gacagcagaa caggcgtggc cgcggtcgga acaacaacca ccgcaagagc cacaatccgc
     3301 tcaccaggaa tttcgaaagc aacggcccgg acgtcaagat ccgcggcacc ccgaccttga
     3361 ttgccgaaaa gtacatgtcg ctcgcgcgcg atgcgctgag ttccggcgat ccggtgctcg
     3421 ctgagaacta tctgcaacac gccgagcact acaaccgaat catcctcgcg gtgcgcgagc
     3481 agcagtcgcc gcagcatggc gacggctcga acggcaacgg catgcggccg cgactgccgg
     3541 gtgagcctct cgatcatgat gacgacgact cgggcgatga tcactcctcg gcgatggacc
     3601 cgcgcttccg ggtgtccgat atgtcggtcg gtcaaccgca accggccccc gcgggcttcg
     3661 gcggccagcc cgacgctcca cgcggaacgg agggggggcg ctacgacggg ccccgcggcg
     3721 gtgagcagcc acgcgcctac gacagccctc gcggcgacga cggtggtcgc tcgcgcgatg
     3781 atcatcgcgg gcagcgccat aacgatggtc ggcacggccg cttccgtgat cgccagtccc
     3841 gcttcgaccg acacgatcgc caggaccgga acgaccgttt cgatcgcggg gatcggggag
     3901 atcgcagtga ccgcggcgac aggtacgatc ggttcgaccg aggcgaccgc tcggatcagc
     3961 ccgatcgcgg tgccatggat cggctctatt ccgatcgtac gggtggcnnn nnnnnnnnnn
     4021 nnnnnnnnna aggcggtgac cggcgcggcg gtgatcggca tggcggtgat cggcatggcg
     4081 gggaacggct gagtgcggaa cggcgagacc cagatcggcc tgcagcggac cggttcacgg
     4141 ccgatcgccc gccccaggat cggcccgggc ctgagcggca cgggatggac cgggaaatcg
     4201 ctcgcgagcg tcagcccact gtcgagcggg aggagccagt tccgat
//
LOCUS       DAORNH010000355         4234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15579_length_4234_cov_3.237617, whole genome shotgun sequence.
ACCESSION   DAORNH010000355 DAORNH010000000
VERSION     DAORNH010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15579_length_4234_cov_3.237617"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2958)
                     /locus_tag="PK264_23185"
     CDS             complement(<1..2958)
                     /locus_tag="PK264_23185"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HRD78805.1"
                     /translation="MRISRFFIDRPIFAAVVSLFITIVGGIAYMSLPVAQFPEITPPT
                     ITVSASYPGASAQTVADTVAAVIEQEINGVDGMIYMYSQSTSDGVLSITVTFDIGTDI
                     DKAQVLVQNRVASAEPRLPEEVRRSGVTVRKNSPDLLLVVHLLSPDRTYDQVYISNYA
                     LLNVRDVLNRIEGVGAVNAFGAREYSMRVWLDPERIAAFNLTAEEVLAALRAQNVQVA
                     GGSIGEPPISATGAFQLSMQLKGRLKDASEFEEIVVKTGAEGRVVRVRDIARVELGAL
                     NYSTYGYQDRYPATVMVVTQQPGSDALKTASAIKKAMAELSKTFPKGLIYRIVYNPTE
                     FIEVSIRELVKTIGEAVLLVVLVVLLFLQTWRATVIPVVAIPISLIGTCAVMLGLGYS
                     INMLSLFGMVLSVGIVVDDAIVVVENVERKLREGLSPLEAARVSMDEVGAALVAIALV
                     LTAVFVPTAFVGGITGAFYRQFAVTIATATVISAFVSLTLSPALAALLFKPHDAHHKG
                     ALLMRPVHFLFRMFNRAFDALAYGYSVLVRGLARVSALMLLVYAGLIYLAVWLVMQTP
                     TGFITPLDRGIIIISMQLPPGSSLERTDAVLKKTNEIVLATPGVKHTNGYTGRNGATF
                     TNATNAAAMFLVLHDALERHAKGLTIQSIAADLRRKLGQIQEANFVVIVPPPVRGIGN
                     SAGFSLRLQDRSGLGSAEFSRVAGELIQALNRPGSGVVGAFTTFQTNTPQLFLDVDRV
                     KAQMLKVPVSNIFETLRIYMGSAYVNDFNMFGRTYRVTAQASGEFRLDPENVTRLRVR
                     NADGQMVPLGSLVTFKEVAGPERVPRYNLFPTIEINGAGAPGTSSGQALEIVQKIAAE
                     ILPEGVAYEWTDLSFQEKRAGRTGYYIFALSVLFVFLALAAQYESWSLPLAIILIVPM
                     CLLAATFGVWLHKQDINILTQIGFVVLIGLAAKNAILIVEFARQLEEQGLDRVEAAVE
                     ACRLRLRPIIMTSL"
     gene            complement(3025..>4234)
                     /locus_tag="PK264_23190"
     CDS             complement(3025..>4234)
                     /locus_tag="PK264_23190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRD78806.1"
                     /translation="PAPVSSPAPAKVPPAASVPAPSGPATTAAPGAAKPAPPPPVVTV
                     SRPAIDEVVEWDEYTARFDAVEMVEVRPRVSGYLTEVAFKDGQLVKKGDLLFVIDPRP
                     FERALEATKAELELAKTRAENAGRDVERGRPLVERKIMSEKVFDDRANAHREAEAQVK
                     VATARMKIAELDLSFTRITAPVAGRIGRALLSPGAYIAGGGAQNTPVLTTIVSQDPIH
                     LYFDVSENNYLKYKRLGQKGAKAGASEVGSAVLVAMPDERDFRHKGLLDFIDNRLDTG
                     TGTLRARAVLENGAALFSPGMFARVRVAGSPRYSAMLLPDSAISSDQASKFVLVVADD
                     GTVSRKPVQLGPIYNGLRIIKVGIGNEDWIVVNGLQRARPGSKVTPKREPIKVSEAPA
                     PAAPAPAKQP"
BASE COUNT          736 a         1305 c         1504 g          689 t
ORIGIN      
        1 gagagacgtc atgatgatcg ggcggagccg gagacggcag gcctcgaccg cagcttcgac
       61 ccggtcaaga ccctgctcct ccaactgacg cgcgaattcc acgatcagaa tggcgttctt
      121 cgccgccagt cctatgagca cgacgaagcc gatctgggtc aggatgttga tgtcctgctt
      181 gtgcagccac acgccgaaag tggcggcgag gaggcacatc ggcacgatca ggatgatggc
      241 aagcggcagc gaccagctct cgtactgggc ggcgagtgcg aggaacacga acagaaccga
      301 cagcgcgaag atgtagtagc cggtgcggcc cgcgcgcttc tcctggaacg agaggtccgt
      361 ccattcgtag gcgacgccct caggcaggat ctcggccgcg atcttctgca caatctcgag
      421 cgcctggcca gacgacgtgc cgggggcacc cgcgccgttg atctcgattg tcgggaagag
      481 gttgtagcgc ggcacgcgct cgggcccggc aacctctttg aaggtgacga gcgagccgag
      541 cggcaccatc tggccgtccg cgttgcgcac gcgcagccgc gtcacgttct cgggatcgag
      601 gcggaactcg cccgacgcct gcgcggtgac gcgataggtc cggccgaaca tgttgaagtc
      661 gttgacgtag gccgagccca tatagatgcg cagcgtctcg aagatgttcg agaccggcac
      721 cttcagcatc tgcgccttga cgcggtcgac atcgaggaag agctgaggcg tgttggtctg
      781 gaaggtggtg aaagcgccga cgacgccgga gccgggccgg ttcaaagcct ggatcagctc
      841 tccggcaacg cgcgagaact cggccgaacc gagaccgctg cgatcctgca gacggagcga
      901 gaagccagcg gagttgccga tgccacgcac gggcgggggc acgatcacga cgaagttcgc
      961 ctcctggatc tggccgagct tgcggcgaag atcggctgcg atcgactgga tcgtcaggcc
     1021 cttggcgtgc cgctcgagtg cgtcatggag gaccaggaac atggccgccg cgttcgtggc
     1081 attcgtaaag gtcgcgccat ttcggcccgt atagccgttc gtgtgcttga cgcccggcgt
     1141 cgccagcacg atctcgttcg tcttcttgag gacggcatcg gttcgctcga gcgaggagcc
     1201 cggtggcaac tgcatcgaga tgatgatgat gccgcggtcg agcggcgtga tgaagccggt
     1261 cggcgtctgc atcacgagcc agacggcgag gtagatgagg ccggcgtaga cgagcagcat
     1321 cagcgccgag acgcgggcga ggccgcgcac gagcacgctg tagccatagg cgagcgcgtc
     1381 gaaggcgcgg ttgaacatgc gaaacaggaa gtggaccggc cgcatgagga gcgcgccctt
     1441 gtggtgcgcg tcgtgcggct tgaagaggag agcggcgagc gccggcgaga gcgtgagcga
     1501 gacgaaggcc gagatgaccg tcgccgtcgc gatcgtcacg gcgaactggc ggtagaaggc
     1561 gcccgtgatg ccgccgacga acgcggtcgg cacgaacacg gcggtgagca cgagggcgat
     1621 ggcgacgagc gccgcaccga cctcgtccat ggagacgcgg gcggcctcga ggggcgagag
     1681 accctcgcgc aacttgcgct cgacgttctc gacgaccacg atcgcgtcgt cgaccacgat
     1741 gccgaccgag agcaccatgc cgaagaggct cagcatattg atcgagtagc cgaggccgag
     1801 catcacggcg caggtgccga tgagcgagat cgggatcgcc accacgggaa tgacggtcgc
     1861 gcgccaggtc tgcaggaaga ggaggacgac gagcacgacg agcagcacgg cctcgccgat
     1921 ggtcttcacg agttctcgga tcgagacctc gatgaactcg gtcgggttgt agacgatgcg
     1981 gtagatgagg cccttcggga acgtcttcga gagctcggcc atcgctttct tgatggcgct
     2041 cgccgtcttc aaggcgtccg agcccggctg ctgcgtgacg accatgacgg tcgccgggta
     2101 gcggtcctga taaccgtagg tcgagtagtt gagggcgccg agctcgacgc gggcgatgtc
     2161 gcgcacgcgc accacgcggc cttccgcgcc cgtcttgacg acgatctcct cgaactcgct
     2221 ggcatctttg aggcggccct tgagctgcat cgagagctgg aaggcgccgg tggcgctgat
     2281 cggcggctcg ccgatggagc cgccggccac ctgcacgttc tgcgcccgga gcgccgcgag
     2341 cacctcttcg gccgtgaggt tgaaggcggc gatgcgctcc ggatcgagcc agacgcgcat
     2401 cgagtattcg cgcgcgccaa acgcgttcac cgcgccgacg ccctcgatac gattgaggac
     2461 gtcgcgcaca ttgaggagcg catagttcga gatgtagacc tgatcgtagg tgcgatccgg
     2521 cgagagcagg tggacgacga gcagcaggtc gggcgagttc ttgcgcaccg tgacgcccga
     2581 gcggcgcacc tcctccggca atctcggctc ggcggaggcg acacggttct ggacgagcac
     2641 ctgcgccttg tcgatgtcgg tgccgatatc gaaggtcacc gtgatcgaga ggacgccgtc
     2701 gctcgtcgac tgcgagtaca tgtagatcat tccgtcgacg ccgttgatct cctgctcgat
     2761 gacggcggca acggtgtcgg cgacggtctg ggcgctagcg ccgggatagc tcgccgagac
     2821 ggtgatggtc ggcggcgtga tctccgggaa ctgggcgacg ggcaggctca tgtaggcgat
     2881 gccgcccacg atagtgatga acagcgagac gacggctgca aagatgggcc ggtcgatgaa
     2941 aaatcggctg atccgcattg gtggacgggc cccgtatcag tcgctcagag tatcgctcgt
     3001 ttgcacgtgt tgcagcttgt tcggttacgg ctgcttcgcc ggcgcaggcg cggccggggc
     3061 cggcgcctcg gacaccttga tcggttcgcg cttcggcgtc accttgctgc cgggccgcgc
     3121 gcgctggagg ccattgacga cgatccagtc ctcgttgccg atccccacct tgatgatgcg
     3181 caggccattg tagatcggac cgagctgcac gggctttctc gagacagtgc cgtcatcggc
     3241 aacgaccagc acgaacttcg aggcctggtc gctcgagatc gccgaatcgg ggagcagcat
     3301 ggccgagtag cgcggcgagc ctgcgacgcg cacgcgcgcg aacatgccgg gcgagaagag
     3361 ggcggcgccg ttctcgagca ccgcgcgcgc acgcaacgtg cccgtccccg tatcgaggcg
     3421 gttgtcgatg aagtcgagca ggcccttgtg gcggaagtcc cgctcgtcgg gcatggcgac
     3481 gagcaccgcc gagccgacct ctgaggcgcc cgccttggcc cccttctggc ctagtcgctt
     3541 gtacttcaga tagttgttct cgctcacatc gaagtagagg tggatcgggt cctggctgac
     3601 gatggtcgtc aggacggggg tgttctgcgc gccgccgccg gcgatgtagg cgcctgggct
     3661 cagcagcgcg cggccgatgc ggcccgcaac aggtgccgtg atgcgcgtga aggagaggtc
     3721 gagttcggcg atcttcatgc gcgcggtggc gaccttcacc tgcgcctcgg cctcacggtg
     3781 cgcgttcgcg cggtcgtcga acactttctc agacatgatc ttgcgctcga cgagggggcg
     3841 gccccgctct acatcgcggc cggcattctc ggcgcgggtc ttggcgagct cgagctcggc
     3901 cttcgtggcc tcgagagcgc gctcgaacgg ccgcgggtcg atgacgaaca ggagatcgcc
     3961 cttcttgacc aactggccgt ccttgaaggc gacctcggtc aggtagccgc tgacgcgggg
     4021 ccgcacctcg accatctcga cggcatcaaa gcgcgccgtg tattcgtccc actcgacgac
     4081 ctcgtcgatg gcgggccggc tcaccgtcac gaccggtggc ggcggcgcgg gcttggcggc
     4141 gcctggtgcc gcagtcgttg cggggccgga gggcgccgga actgaagcag ctggcggaac
     4201 tttcgcaggt gcgggcgacg aaactggggc cggc
//
LOCUS       DAORNH010000356         4233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15587_length_4233_cov_2.782910, whole genome shotgun sequence.
ACCESSION   DAORNH010000356 DAORNH010000000
VERSION     DAORNH010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15587_length_4233_cov_2.782910"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK264_23195"
     CDS             <1..506
                     /locus_tag="PK264_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRD78807.1"
                     /translation="PEALPSVKELMAQLIDTLPENMRSYWQRENPIELRPVDVSRYLS
                     RDPQRPVQQHIWLKANGPLPDDARLHQCVLAYASDFTLLDTALIAHGKLMFDRDIQLA
                     SLDHAQWFHRPFRADEWLLYVQDSPSAHGARGFCRGSVFTRDGILVSSAAQEGLTRQR
                     RTDFLPE"
     gene            695..1033
                     /locus_tag="PK264_23200"
     CDS             695..1033
                     /locus_tag="PK264_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRD78808.1"
                     /translation="MKLITAIIKPFKLEDVRNALTEIGVQGMTVSEVKGYGRQKGHRE
                     IYRGAEYAVAFLPKIKIEAVVASSDVDKALAAIVGAAKTGQIGDGKIFISSVERTIRI
                     RTGEQDESAI"
     gene            1087..>1644
                     /locus_tag="PK264_23205"
     CDS             1087..>1644
                     /locus_tag="PK264_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRD78809.1"
                     /translation="MKRLLLLLAIAAAAIGIALPDLALAADAPKPNKGDVAWLTTATA
                     LVLLMTVPGLALFYGGLVRSKNMLSVLMQVFCTVCIVAVLWVVYGYSLAFTEGSGALN
                     SYIGGMSRVFLKGMDADATAATFSAGVVIPEYSFIAFQMTFAAITPALVVGAFAERMK
                     FSAVVLFVPLWLTFVYIPIAHMVWYW"
     assembly_gap    1645..1725
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1726..2547
                     /locus_tag="PK264_23210"
     CDS             <1726..2547
                     /locus_tag="PK264_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HRD78810.1"
                     /translation="AKLAEAKANQGMIYNWGAIDFAGGTVVHINAGIAGLVGALIIGR
                     RVGFGREVMAPHSLTMTMIGGAMLWVGWIGFNAGSALEATGGTALALLNTMVATAAAA
                     LAWILVENLGKGHPSMLGAVSGAVAGLVAITPAAGVAGPMGSIVLGFVAGIVCYVFCT
                     TIKNTFRYDDALDVFGVHCIGGIVGAIGTAIVAAPMLGGTGVMDYETGKIGEYVIMAQ
                     LWIQAKAVAVTLVWSGVISALIFLVISAIVGLRPSTDQEREGLDLVDHGERAYNY"
     gene            2820..>3381
                     /locus_tag="PK264_23215"
     CDS             2820..>3381
                     /locus_tag="PK264_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78811.1"
                     /translation="MSKILASLIAGAAISIAGALPAMADGMPKKAAPAYHAAPPAGDC
                     CCGGDKFGGAFIGVHAGMGSLTSTLTDRDNFVDRASVEHVEDGFSFGAQIGRNWVRCN
                     VVFGVEADISIADYDSRNIYDAGNETVSRSTNWIASLRTKSGLAMGDLFIYATGGFAF
                     ADFETRYSSIAPAGAFTIDGTRVGWVA"
     assembly_gap    3382..3399
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3860..3991
                     /locus_tag="PK264_23220"
     CDS             3860..3991
                     /locus_tag="PK264_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78812.1"
                     /translation="MSQIIAGLVAGAVIALTFSLTAKAVEAPQSLVAASPVSVIVTR"
BASE COUNT          717 a         1320 c         1259 g          838 t
ORIGIN      
        1 cgcccgaggc tctgccctcc gtcaaggagc tgatggcgca gctcatcgac acgctgccgg
       61 aaaacatgcg cagctattgg cagcgcgaga acccgatcga gctgaggccg gtggacgtct
      121 cgcgctacct cagccgggat ccgcagcgcc ccgtgcagca gcacatctgg ctcaaggcca
      181 atgggccgct gccggacgat gcacggctcc accagtgcgt gctcgcctac gcgtccgact
      241 tcacgctgct cgatacggcg ctgatcgccc acgggaagct gatgttcgat cgcgatatcc
      301 agctggcgag ccttgatcat gcgcaatggt tccatcgccc gttccgcgcc gacgaatggc
      361 tcctctatgt tcaggacagc ccgagcgccc acggcgcgcg cggcttctgc cgcggaagcg
      421 tgttcactcg ggatggcatc ctcgtctctt ctgcggcgca agaagggctg acccgccagc
      481 ggcgcacaga ttttctgcct gaataatagg ctaatgcaga tttctgatta atttttagtc
      541 gcttgcttga ccgcaatggc atgggcgaca cggctgactt cggcagggtg tttcaagtcg
      601 ttacgcctgc cgtcgtgaac tggcacagcg cttgctagcg cgtctcattg tggatcgcac
      661 tgcacaatgt gcggtcgcag caaggggaca agccatgaag ctgatcacgg ccatcataaa
      721 gccgttcaag ctcgaggacg ttcgcaatgc cctgaccgag atcggtgtgc agggcatgac
      781 cgtcagcgag gtcaaggggt acggacggca gaaggggcat cgcgagatct atcgcggtgc
      841 ggaatatgcc gtcgccttcc tgccgaaaat caagatcgag gccgttgttg ccagctccga
      901 cgtcgacaag gcgcttgcgg cgattgtcgg cgcggccaag accggccaga tcggcgacgg
      961 caaaatcttc atctcgagcg tcgagcgcac catccgcatc cgcacgggcg agcaggacga
     1021 atccgccatc tgagcgcgcc tgcgccgcga tccacgatcc attccgaaca tgacgggtcc
     1081 catcacatga aaagacttct cctcctcctg gcgattgccg ccgcagcaat cggtatcgcc
     1141 cttcccgatc tcgcgctcgc cgccgatgcg ccgaagccga acaagggcga cgtcgcctgg
     1201 ctgaccaccg cgaccgcgct cgttctgctc atgaccgttc ccggcctcgc gctcttctac
     1261 ggcggcctcg tccgctcgaa gaacatgctc tcggtgctga tgcaggtgtt ctgcacggtc
     1321 tgcatcgtcg cagtgctatg ggtcgtctac ggctacagcc tcgccttcac cgagggtagc
     1381 ggtgcgctca acagctacat cggcggcatg tcacgcgtgt ttctgaaagg catggacgcc
     1441 gacgcgactg cggcgacgtt cagtgccggc gtcgtgatcc ccgagtattc cttcatcgcc
     1501 ttccagatga cgttcgcggc aatcacgccc gcgctggtcg tcggcgcctt tgccgagcgc
     1561 atgaagttct cggcggtcgt gctgttcgtg cccctctggc tcaccttcgt ctacatcccg
     1621 atcgctcaca tggtctggta ctggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccaa gctcgccgag
     1741 gcgaaggcca accagggcat gatctacaat tggggcgcga tagacttcgc cggcggtacc
     1801 gtcgtgcata tcaacgcggg catcgcgggc ctcgtcggtg cgctcattat cggccgccgt
     1861 gtcggattcg gccgcgaggt gatggccccg cactcgctca ccatgaccat gatcggcggc
     1921 gcaatgctct gggtcggctg gatcggcttc aacgcgggtt ccgcgctcga ggcgaccggc
     1981 ggcacggcgc tcgctctcct caacacgatg gtcgcgacgg cagcagcagc gctcgcctgg
     2041 atcctcgtcg agaacttggg taagggccat ccctcgatgc tcggcgccgt ctctggcgct
     2101 gtcgcaggcc tcgttgcgat cacgccggct gccggtgtcg ccggcccgat ggggtcgatc
     2161 gtccttggct tcgtcgccgg cattgtctgc tacgtgttct gcaccacgat caagaacacg
     2221 tttcgctacg acgatgcgct cgacgtcttc ggcgtgcact gcatcggcgg catcgtcggc
     2281 gccatcggca cggcgatcgt tgcagcgccc atgctcggcg gcaccggtgt gatggattac
     2341 gaaaccggca agatcggcga gtacgtgatc atggcgcagc tctggatcca ggcgaaggcc
     2401 gtggctgtga cgctcgtgtg gtccggcgtc atctcagcgc tgatctttct cgtcatctcc
     2461 gcgatcgtcg ggctccggcc gtcgacggat caggagcgcg agggcctcga cctcgtcgat
     2521 cacggtgagc gcgcctataa ctactaattg ctctgggcaa atgcgcccaa tgcgaagctt
     2581 gaaagtctgg ccggcagtcg ggttcgccct ggctgccggt ttctcatttt ggccgcgact
     2641 tgaggcgatc gcagcgcaac cgcaacagat gtcgccgaat tggggagcgt tctgaatgcg
     2701 atgcatcgac attttccacg aatcatccac aactcgccaa cagttaacaa tgcgtttccg
     2761 ttcatgcgtt gtgccgcgcc ggggtctcgc cccagcagaa ccctgacagg gaaaattcaa
     2821 tgtcgaagat ccttgctagc ctgatcgcgg gtgccgcgat ctctatcgcc ggggcgctgc
     2881 ccgcaatggc cgacggcatg ccgaagaagg cagcccccgc ctaccacgcc gcgccgcctg
     2941 caggcgactg ctgctgcggc ggcgacaagt tcggcggtgc ctttatcggc gtccatgccg
     3001 gcatgggtag cctcacgtcg actctgaccg atcgggacaa cttcgtcgac cgcgcatcgg
     3061 tcgagcacgt cgaggacggc ttctcgttcg gcgcccagat cggtcgcaac tgggtgcgtt
     3121 gcaacgtcgt gttcggcgtc gaggccgata tctcgatcgc cgactatgac agtcgcaaca
     3181 tctatgacgc aggcaacgag acagtgtccc gctcgaccaa ctggattgcg tctctgcgca
     3241 cgaagagcgg tctggccatg ggtgacctct tcatctatgc gaccggcggt ttcgcctttg
     3301 ccgacttcga gacccggtac tcgagcattg ctccggcagg tgccttcacg atcgacggta
     3361 ctcgagtcgg ctgggtcgca gnnnnnnnnn nnnnnnnnng gtcggatcgc gtgcgcatca
     3421 caggcgacgt gctctactac gatttcggta cggattcggc gaggatcggc aatgaccgct
     3481 tcgacgagca tcactcgctc tgggtgtcgc gcatcggcct caatttcgcg ctcggcggcc
     3541 atcgcagtca cgacgtcggg ccgttgaagt aagcggcgtc cccatcaccg tcacatgggc
     3601 tgcgccggag catccggcgc agcctttttt gtcgtgccgg gccaaattcc gataggcacc
     3661 ctcgatcggg cgactcaggg cttcgctcgg atcggtatca atcccagcgt caataggatc
     3721 ggccgatgcg ctgaatggtt gtcgacaatt tctcgaatca ggagcattat gttggggaca
     3781 aggttcgttt ggcgttgtca tctgcacgct gcccgatttg ccgcgattct gcggccgtca
     3841 tcggaagacg agaaccttca tgtcccagat catcgctgga cttgtcgccg gggcggtcat
     3901 tgccctcaca ttctcgctga ctgcaaaagc ggtggaggcg cctcagtcgc tggtggccgc
     3961 ttcacctgtt tcagtgattg tcacgcgctg aaaatgattg tcagcaaagc acggggcgac
     4021 cgcgcgatct caattcggca cgcattttac gaaatctggc aacaaatcga taaccaaacc
     4081 tgaggtgggt cgaggctagg gtcgcgaaca ggagttcgtt cctgtcccgc gttggagccc
     4141 ccgcctcatg acccccgcat tcaagcgatt tccgaggagc atcggccttg gcgccgctgc
     4201 cgcaatgcca ctcatcttcc tgccgatcgg tgt
//
LOCUS       DAORNH010000357         4213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15702_length_4213_cov_2.656325, whole genome shotgun sequence.
ACCESSION   DAORNH010000357 DAORNH010000000
VERSION     DAORNH010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15702_length_4213_cov_2.656325"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    208..354
                     /estimated_length=147
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <355..1012
                     /locus_tag="PK264_23225"
     CDS             <355..1012
                     /locus_tag="PK264_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78813.1"
                     /translation="VVSLGYVERPSTEGQPFDAPMIADLDDRLRAIISAEAGRSLNVL
                     PTNVLGVPLTAVYGMVQRRDAKELKFLKKDEFDALMWLTGVKRPGGEQIVTLEIFLPW
                     LNPPCSRVLQIKVMAASSAPLVPKKPPVTAPSAGGWLQKEVCIGGYALLADRHFCGAV
                     LREDDTGFDIVVTVPSASFMNPIRHLDECSANRDVRTLQPDLRLSIPRHCQGRFWLKR
                     "
     gene            complement(1030..>1374)
                     /locus_tag="PK264_23230"
     CDS             complement(1030..>1374)
                     /locus_tag="PK264_23230"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD78814.1"
                     /translation="RLGAFYTRHDVMLSPTTFRPAEPWGNYNLGRGDVTMETLIEKVF
                     RGTCQYSLPHNIMGTPAMSLPLAMTKAGLPVGVQLAGKPAEEHVILQLATALEQAMPW
                     ADRVPPLHVTKA"
     assembly_gap    1375..1494
                     /estimated_length=120
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1495..>1948)
                     /locus_tag="PK264_23235"
     CDS             complement(<1495..>1948)
                     /locus_tag="PK264_23235"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD78815.1"
                     /translation="PASYCGGVGLKPSRGRVSFAPMVDENGYGLAQNFVQAKTVRDAA
                     AALDCLAKPQPGDPFVIPRPSEPWAVVARHKAPKLRIGWTSAPLMGLITDPEVRAAIV
                     ATAKTLEDMGHIVEESDPDLGGMEAMRGMSDAWFFGFDLRLEGYSKRSG"
     assembly_gap    1949..1962
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1963..2485)
                     /locus_tag="PK264_23240"
     CDS             complement(<1963..2485)
                     /locus_tag="PK264_23240"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HRD78816.1"
                     /translation="MPAMSLAEYARHDALGLAELVAKKAVTPRELAETASAAIAKANP
                     EINAVVETYADRIGALDEKSLGNGPFRGVPFLMKDVFGHEKGRKIEFGSRLCKGMIGE
                     QDTAYAELVRAAGFNILGRSAAPEYSMAGTTEGALYGNTSTPWKKGFSAGGSSGGAQA
                     AVTSGMVPIAHGSD"
     gene            complement(2524..3210)
                     /locus_tag="PK264_23245"
     CDS             complement(2524..3210)
                     /locus_tag="PK264_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRD78817.1"
                     /translation="MSTKRKILIVDDEDELRDSLRDQLALVDEFEVSTANSAAKGMDL
                     AKGDNWDLLILDVGLPDMDGREACKLLRRANFKAPVIMLTGHTSDSDMILGLDSGAND
                     YVTKPFKFSVLLARIRAQLRQHEQSEDAVFAIGQYSFKPASKLLVDEKGGKVRLTEKE
                     TSILKFLFRAGEKVVSRDVLLHEVWGYNAGVTTHTLETHIYRLRQKIEKDPSNAELLV
                     TEAGGYKLVP"
     gene            3380..3880
                     /locus_tag="PK264_23250"
     CDS             3380..3880
                     /locus_tag="PK264_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRD78818.1"
                     /translation="MALSRASRRGWLRLGALVMPCALGRSGSRVMKREGDGATPIGRY
                     AIRALLYRPDRGVPAPSWLPRRALGRRHGWSDDPCDRNYNRPVRRPYPASAEELWRND
                     RLYDHVIVIDHNERPRIRGHGSAVFMHVARPDMAPTAGCVALKPSDMRRLLARLRRPA
                     ELVIRF"
     gene            complement(3907..>4213)
                     /locus_tag="PK264_23255"
     CDS             complement(3907..>4213)
                     /locus_tag="PK264_23255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78819.1"
                     /translation="GELHEHVDMSLLGPDIATPPPIPTTAIYSRTDGIVPWRSCVQAR
                     GHLAESVEVYSSHMGLCVNGAALYAIADRLALPEGHWRPFDTSGIRQMFYGRSEPDA"
BASE COUNT          624 a         1260 c         1336 g          712 t
ORIGIN      
        1 tcgatcatgt cgttagcggc catgttcggc gcggtgtgcc gagaagtcca tggcaaagtt
       61 ggccggcctc ctggccggcc gcgagggtgc catcgtcccc ggttgccaaa acccagcgtc
      121 acgttatcta acgagagtga gattcgcgcc tgggtttgtt gtctgttgtc gcggaggggg
      181 aagcgatgcc tcgcatttcg aagcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtcgt
      361 cagcctcggc tacgtcgagc ggccctcgac cgaagggcag cccttcgatg cgccgatgat
      421 cgcggacctc gacgatcggc tgcgcgccat catctccgcg gaggcggggc gcagcctcaa
      481 cgtgctgccg acgaacgtcc tcggcgttcc cctcacggcc gtctatggga tggtgcagcg
      541 gcgcgacgct aaggaattga agttcctcaa gaaggacgaa ttcgatgcgc tgatgtggct
      601 gaccggtgtg aagcgccccg gcggcgagca gatcgtgacg ctcgagatct tcctgccgtg
      661 gctcaacccg ccctgctcgc gcgtgttgca gatcaaggtc atggccgcgt cctcggcgcc
      721 gctcgtgccg aaaaagccgc cggtgacggc gcccagtgca ggagggtggc tgcaaaaaga
      781 ggtgtgcatc gggggctacg cgctgctggc cgatcgccac ttctgcggcg ctgtgctgcg
      841 cgaggacgat acgggcttcg acatcgtcgt caccgtccca tcggcctcgt tcatgaaccc
      901 gatccgccac ctcgacgaat gtagcgccaa tcgcgacgtg cgcacgctgc agccggacct
      961 gcggctgtca atcccgcggc attgccaggg gcgcttctgg ctcaagcgat aggccgtcgc
     1021 ctccggcggc tatgccttcg ttacgtgcag cggcggcacg cggtcagccc agggcatggc
     1081 ctgctcgagc gcggtcgcga gctgcaggat cacgtgctcc tccgcgggct tccccgcgag
     1141 ctgcacgccg accgggaggc ccgccttggt catcgcgagc ggcagcgaca tcgccggcgt
     1201 ccccatgatg ttatggggca gcgaatactg gcacgtaccg cggaatacct tctcgatcag
     1261 cgtctccatg gtgacgtcgc cgcggccgag attgtagttg ccccagggtt ccgccggacg
     1321 gaaggtcgtc ggggatagca tcacgtcgtg ccgcgtatag aaggcgccga gccgnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcccg
     1501 agcgcttcga gtagccctcg aggcgcagat cgaagccgaa gaaccaggcg tccgacatgc
     1561 cgcgcatggc ctccatgccg ccgaggtcgg gatcgctctc ctcgacgatg tggcccatgt
     1621 cctcgagcgt cttggccgtc gcgacgatcg cggcgcgcac ttcagggtcg gtgatgaggc
     1681 ccatcagcgg cgcggaggtc cagccgatgc gcaatttcgg tgccttgtgt ctggcaacca
     1741 cggcccacgg ctccgacggg cgtggaatga cgaaagggtc gcccggctgg ggttttgcca
     1801 ggcagtcgag ggcggctgcg gcgtcgcgca ccgtcttcgc ttgtacgaag ttctgggcga
     1861 ggccgtagcc gttctcgtcg accatcggcg caaacgagac gcgaccgcgc gagggcttta
     1921 ggccgacgcc gccgcagtag cttgccggnn nnnnnnnnnn nntgtcggag ccgtgcgcga
     1981 tcggcaccat gccagaagtt acggcggcct gcgcgccgcc cgacgagccg ccggccgaaa
     2041 agcccttctt ccaaggcgtc gacgtattgc cgtaaagtgc gccttcggtc gtgccggcca
     2101 tcgagtactc gggcgcggcg ctccggccga ggatgttgaa ccccgccgcc cggacgagct
     2161 cggcataggc cgtatcctgt tcaccgatca tgcccttgca gagccgcgag ccgaactcga
     2221 ttttgcggcc cttctcatgg ccgaacacgt ccttcatcag gaacggcacg ccgcggaacg
     2281 ggccgttgcc gagcgacttt tcgtcgaggg cgccgatgcg gtcggcatat gtttcgacga
     2341 cggcgttgat ctctgggttg gccttggcga tggcggcaga ggcagtctcg gcaagctcgc
     2401 gcggcgtcac ggccttcttt gcgacgagct cggcgagccc cagggcatcg tgacgtgcat
     2461 attcggcgag tgacatggcg ggcatgactt actccacgca ttcaggcgtt cagggcctca
     2521 ggatcagggc acaagcttgt agccgcccgc ctcggtgacc agcagctccg cgttggaggg
     2581 gtccttctcg atcttctggc ggagccggta aatgtgggtc tcgagcgtgt gcgtcgtcac
     2641 tccggcattg tagccccaca cctcgtgcag cagcacgtcc cgcgagacga ccttttcccc
     2701 ggcgcggaac aagaacttca ggatcgatgt ctccttctcg gtgaggcgaa ccttgccgcc
     2761 cttttcatcg acgagcagct tcgaggccgg cttgaaggag tactggccga tcgcgaacac
     2821 ggcatcctcg ctctgctcat gctggcggag ctgcgcgcgg atgcgggcga ggaggaccga
     2881 gaacttgaag ggcttggtca catagtcgtt ggcgccggaa tcgaggccga ggatcatgtc
     2941 cgagtccgag gtgtgccctg tcagcatgat gacgggcgcc ttgaagttcg cccgacgaag
     3001 cagcttgcag gcttcgcggc cgtccatgtc gggaaggccg acgtccagga tgagcagatc
     3061 ccagttgtcg cccttggcga ggtccatgcc cttggcggcc gaattggcgg tcgagacctc
     3121 gaactcgtcg acgagcgcca gctgatcgcg cagcgagtcg cgtagctcgt cctcgtcgtc
     3181 gacgatcagg atcttgcgtt tcgtgctcat ggtattggtc tcacagtccg gtcattggct
     3241 gcggcagaca acgccgaagc tagcaccggg gcagcggagt gaacaacaat cgttcggcgc
     3301 tatcgacatg ttacttgcat tgccgtctcg tgatcgacct ctgcgtgtac cggcgcgacc
     3361 gacgcagcgg ctcgtcgtca tggccctgag cagggcttcg cgacgcgggt ggcttcgcct
     3421 cggagcgctc gtgatgcctt gtgcgctcgg gcgaagcggg tcgcgggtca tgaagcggga
     3481 aggcgacggc gcgacgccga tcggacgcta tgcgatccgg gcgctgctct atcggccgga
     3541 ccgaggcgtg ccggcaccct cttggctgcc gcgcagggcc ctcggccgac gccacggctg
     3601 gtcggatgat ccctgcgatc gcaactacaa tcggcctgta cggcgtccct acccggcgag
     3661 cgccgaggag ctctggcgca atgatcgcct ctacgaccac gtgatcgtca tcgaccacaa
     3721 cgagcgaccg cgcatccgcg gccatggcag cgcggtcttc atgcatgtcg cacgccctga
     3781 tatggcgccg acggccggat gcgtcgcgct gaagccatcc gacatgcgcc gtctgctcgc
     3841 gcgcttgcgt cgacccgccg agctggtgat ccgcttttga gcgccagtac tgacgagatg
     3901 cggccttcag gcgtcgggtt cggaccggcc gtagaacatc tggcggatac cggacgtgtc
     3961 gaacgggcgc caatggcctt cggggagcgc gagcctgtcg gcgatggcgt agagcgctgc
     4021 gccattgacg cagagaccca tatggctcga gtagacctcg acgctttccg cgaggtgtcc
     4081 tcgcgcctgc acgcaggagc gccatgggac gatgccgtcg gtgcggctgt agatcgcggt
     4141 ggtcggtatc ggcggcggcg tcgcgatatc gggtccgaga agcgacatgt cgacatgctc
     4201 gtgcagctcg ccg
//
LOCUS       DAORNH010000358         4195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15803_length_4195_cov_2.719082, whole genome shotgun sequence.
ACCESSION   DAORNH010000358 DAORNH010000000
VERSION     DAORNH010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15803_length_4195_cov_2.719082"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="PK264_23260"
     CDS             complement(<1..1310)
                     /locus_tag="PK264_23260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78820.1"
                     /translation="MRCNPLRWLWGLIPVAMLSWLALLGEHERIESDLGARVREALTR
                     AGLGQWATASFKGRDGRISGSALEDSEPAKATEIARGIWGVRIVDDKSSLVEKVDRFE
                     FKAVRADNRVRLTGFAPSPKARAEIAARSAWIAAMAKAAFPNTTVDDQLVIKRGAPAD
                     WLGGVSFGLRQLSELASGEVSLEGTALTVTGLARTPGTLRAVRSALKGSLPRGISLKD
                     ERIRAPQVVPYTWQAAYDGKKVVMSGHVPSEAVRADLLDAVKKAIPAAVVEDGQLEYA
                     DGAPADWPKVAILALRELGRLESGTASLSGTDISFTGLAGPQAVADNVRQALQALPKG
                     YKLAEKVTYRVATPRPKSPYVSVASVDNGSLLLSGYVPSAAAKSAVGDHAKAQFPSLR
                     IINELEIADGAPGGWLRCMEAGVTGLKRVGNGRVRMTDRLLEVDG"
     gene            1859..4090
                     /locus_tag="PK264_23265"
     CDS             1859..4090
                     /locus_tag="PK264_23265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRD78821.1"
                     /translation="MTHLELQLDDDTSRAARHKRRVPVLMPVALDQTYDYIVPDDVEV
                     APGAFCLVPFGPQQRIGIVWDRPIGPAGDVAETKLKPLLSRLEVPPLPPTSMRFAEWV
                     GRYTISHVGMVVRMMMSASAAFEPVKPRFGVRAVQGAVLPSRITPARERALAIAADGA
                     VRAKGALALEAQCSTAVIDGLVAAGALIELAMPEKRFPRPDPNFATVDFSAEQAAAVH
                     ALVASVDARAFSVSLLDGVTGSGKTEVYYEAVARCLAAGRQALIMLPEIALTSQFMSR
                     FQARFGVPPAEWHSALSPAERARTWRAAATGEARVIVGARSALFLPFADLGLIVVDEE
                     HDQGFKQDDRVHYQGRDMAVVRGSIGGAAVVLASATPSIESHVNARSGRYRHLVLPGR
                     FSGIELPAITPVDMRRTPPERGRWLSPVLTDAVGETLARGQQALLFLNRRGYAPLTIC
                     RGCGHRMECPQCTAWLVEHRFRNRLNCHHCGFSLPIPEKCPKCSEPSSLIACGPGVER
                     IAEEVRERFPEAKVVLLSSDLVPGLEEMRKLVKTIESGETHIIIGTQIVAKGHHFPNL
                     AMVGVVDGDLGLAQGADPRAGERTFQLLHQVTGRAGRAFAQGRGFIQTYNPEHPVMEA
                     ILSGDREAFLEREIRQRQAGLLPPYGRLAALIVSARQKEVAEAMAREVARRAPPADTV
                     EVLGPAEAPLAVIRGRHRWRLLAKAPRDVDLQGYIRAWLDALPEIRGDLRLTVDIDPY
                     SFL"
BASE COUNT          637 a         1451 c         1392 g          715 t
ORIGIN      
        1 ccgtcaacct cgagcaggcg atccgtcatg cgcacgcgcc cgttgccgac gcgcttcagc
       61 ccggtgacgc cggcctccat gcagcgcagc caccctcccg gagcgccatc ggcaatttcc
      121 agctcattga tgatgcgcaa gctcgggaac tgcgccttgg cgtgatcgcc gaccgccgac
      181 ttggccgcag cgctcggcac atagcccgag agcagcaacg agccgttgtc caccgaggcg
      241 acggacacat aaggcgactt cggccgtgga gtcgctacgc gatacgtgac cttctcagcg
      301 agcttgtagc ccttcggcaa ggcctgcagg gcttggcgga cattgtcggc aacggcctgc
      361 ggaccggcga gcccagtgaa cgatatgtcg gtacccgaga gcgacgctgt gccgctctcg
      421 aggcggccga gctcgcgcag ggccagaatg gccactttcg gccagtcggc cggggcgcca
      481 tccgcatact cgagttggcc atcctcgaca acggccgccg gaatagcctt ctttaccgca
      541 tccaggaggt cggcgcgaac ggcctcgctc ggcacatgac cgctcatgac gactttcttg
      601 ccgtcgtagg cggcctgcca cgtgtagggc acgacctgcg gcgcgcggat gcgctcgtcc
      661 ttcaatgata tgccgcgcgg caggctgccc ttgagggcgc tgcggaccgc acgcagtgtg
      721 ccgggcgttc ttgcgagccc ggtcacagtc agtgccgttc cctcaaggct tacctcgccg
      781 gaggcgagtt cgctgagctg gcggaggccg aagctgacgc cgccgagcca atcggccggc
      841 gcgccgcgct tgatgacgag ctgatcgtcg acggtcgtgt tcgggaatgc cgcctttgcc
      901 atggccgcga tccaagcgga gcgggccgcg atctcggcgc gcgccttcgg gctcggcgca
      961 aagccggtca agcgcacgcg gttgtcggca cgcacggcct tgaactcgaa gcggtcgacc
     1021 ttctcgacga gcgacgactt gtcgtcgacg atgcgcacgc cccagatgcc gcgggcgatc
     1081 tcggtggcct tggccggttc gctgtcctcg agcgcgctcc cagagatgcg cccgtcgcgc
     1141 cccttgaatg atgcggtggc ccactggccg aggcccgccc gtgtcaaagc ctcgcggacg
     1201 cgcgcgccga ggtcgctctc gatgcgttca tgctcgccga gcagggcgag ccagctcagc
     1261 atcgcgaccg gaatcaggcc ccacagccag cgcaacggat tgcatctcat gccacatgca
     1321 ctccccaaac cttctccgtc gatgccgtgc cgagcggcac tcgcgtgccc gtcgtcgggc
     1381 ccgattatgg acccggtgct cgctgaactg gcaccattgc tcccggccgc gtcgctcccg
     1441 gccgcgccaa tctcgtgctc gtttcgctct gcgtgagcag aagcgtgcga gtgagccggc
     1501 ggaggcaagc gtgaggcgcg gcgataagcg acgcccttat agacaatgca gcccctcatt
     1561 ccaagctagc cagaggtcgg cgattggtcg cggcagcagc ggcggtattg agcataacct
     1621 ttggaaacaa catgttttta gcggataatc agcagcacgc cggaggagtt gttcccgcgc
     1681 aagcaagtcg cggatcgcga atcgtgacgc ggtatttttg ccacatatcc attgttgttt
     1741 tctcaacatg agccgttttg cacaatgtat tgcaactgac gacgctgcct tcgcaccgcc
     1801 gcgctcgcgc gtctaaggtt cagcgggacg attcgatccc ggaccacgat cacgtgccat
     1861 gacgcacctc gagctacagc ttgacgacga cacgagcagg gctgcgcgcc acaagcggcg
     1921 cgtgccggtg ctgatgcccg tcgcgctcga ccagacatac gactatatcg tcccggacga
     1981 cgtcgaggtc gcacccggcg cgttttgcct cgtgccgttc gggccgcagc agcggatcgg
     2041 catcgtctgg gatcgtccta tcggaccagc gggcgacgtt gccgagacca agctgaagcc
     2101 gcttctgtcg cgcctcgagg tgccgccgct gccgccgacc tcgatgcgct ttgccgagtg
     2161 ggtcggccgc tacacgatct cgcatgtcgg catggtggtg cgcatgatga tgagcgcctc
     2221 ggccgccttc gagccggtga agccgcgctt cggcgttcgc gctgttcaag gggccgtgct
     2281 gccctcgcga attacgcccg cacgcgagcg ggcgcttgcc atcgccgccg acggcgcagt
     2341 tcgcgccaag ggcgcactcg cgctcgaggc gcaatgctca acagccgtga tcgacggtct
     2401 cgtcgcggct ggcgcgctca tcgagctcgc catgcccgag aagcgctttc cccgccccga
     2461 tccgaatttt gcgacggtcg atttcagcgc cgagcaggcc gctgccgtcc acgcgctggt
     2521 ggcgagcgtc gatgcgcgtg cgttctcggt ctcgctgctc gacggtgtca ccggctcggg
     2581 caagaccgag gtctactacg aggccgtggc gcgttgtctc gccgctggac ggcaggccct
     2641 gatcatgctg cccgagattg cgctgacgag ccagttcatg agccgcttcc aggcccgctt
     2701 cggcgtgcct ccggccgagt ggcattcagc gctttcgccg gctgagcgcg cccgcacttg
     2761 gcgggcggcg gcgacgggcg aggcgcgcgt gatcgtcggc gcccgctccg cactcttcct
     2821 gccgttcgcc gatctcggct tgatcgtcgt cgacgaggag cacgaccagg gcttcaagca
     2881 ggatgaccgc gtccactacc agggccgcga catggcggtg gtgcgcggct cgatcggggg
     2941 agcggccgtg gtgcttgcct cggcgacgcc ctccatcgaa agccacgtca acgcgcggtc
     3001 agggcgatat cgccacctcg tgctgccggg ccgattctca gggatcgagc taccggcgat
     3061 cacgccggtc gacatgcggc ggacgccgcc ggagcgcggg cgctggcttt cgccggtgct
     3121 gacggacgca gtcggcgaga cgctcgcgcg cgggcagcag gcgctcctgt tcctgaaccg
     3181 gcgaggctac gccccgctca cgatctgcag gggctgcggt caccgcatgg aatgcccgca
     3241 gtgcaccgcc tggctcgttg agcaccgctt ccgcaaccgg ctcaactgcc atcattgcgg
     3301 cttctcgcta ccaatcccgg agaagtgtcc gaagtgcagc gagccatcat cgctcattgc
     3361 ctgcggcccg ggggtcgagc gcatcgcgga ggaggtgcgt gaacgcttcc cggaggccaa
     3421 ggttgtgctc ctgtcgtcgg atctcgtgcc ggggctcgag gagatgcgca agctcgtgaa
     3481 gacgatcgag tcgggcgaga cgcacatcat catcggcacg cagatcgtcg ccaagggcca
     3541 ccacttcccg aacctcgcga tggtcggtgt cgtcgacggc gatctcggcc tcgcgcaagg
     3601 cgccgatccg cgcgcgggcg aacgcacctt ccagctgctc catcaggtca cggggcgcgc
     3661 cggacgcgcc ttcgcgcaag gccgcggctt catccagacc tacaatcccg agcatccggt
     3721 gatggaggcg atcctctcgg gcgaccgcga ggcgttcctc gagcgcgaga tccgccagcg
     3781 ccaggccggc ctgctgccgc catacgggcg gctcgcggcg ctgattgtct cggcgcgtca
     3841 gaaggaggtc gcagaagcca tggcgcgcga ggtcgcgcgg cgcgcgccac ctgcagacac
     3901 tgtcgaggtg ttgggtccgg ccgaggcgcc tctcgcagtc atccgtgggc gtcatcgctg
     3961 gcgcctgctc gccaaggcgc cgcgcgacgt cgacctgcag ggctacatcc gggcgtggct
     4021 cgatgcgctt cccgagatcc gcggcgatct ccgcctgacg gtcgatatcg acccctactc
     4081 gttcctctga acgaggctgt cgaccgctgc cctcaggctt cgccgttccg gggctgcgcc
     4141 ttggtcggcg gcatcacctc gacgattggc ctgaccgggt cgggcgccgc cgcgc
//
LOCUS       DAORNH010000359         4177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15886_length_4177_cov_2.496604, whole genome shotgun sequence.
ACCESSION   DAORNH010000359 DAORNH010000000
VERSION     DAORNH010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15886_length_4177_cov_2.496604"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PK264_23270"
     CDS             complement(<1..856)
                     /locus_tag="PK264_23270"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HRD78822.1"
                     /translation="MSQSSQTLAVSFRRSEDGTIAMIFAICAFFLVMVTGLAIDVGRA
                     MHANQKISDALDSAALAAAKGMRDQNLTDGEIDDLAKRYFAINMDGTGANYANVSELR
                     VAINRGNNSIALDVDADVPTLFANIAGISKISFPRSAVAIYDRKDVEVGLQLDVTGSM
                     HGRKLEDLKVAVVDLLDILMPDAGTTNEVRVALAPFSAGVNAGGLAGAVSNGRSSNGC
                     VYERNNSADQDTDVAPVGKAALKGNLDLPGAGACSPNARVLPLSVHVVPGITSLQVLT
                     AAHAVPLNE"
     gene            complement(993..1838)
                     /locus_tag="PK264_23275"
     CDS             complement(993..1838)
                     /locus_tag="PK264_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD78823.1"
                     /translation="MTHAALLLVLAEGLLTIMDGIIKGLAQRYPTLQIAGLRFGFGFV
                     WAAALVAVLRPGWPTLETVKANAVRSALSVATATLFFYALGQLPLAEVFALSFIAPIF
                     MVLLGVIVLGEQLEGRIAAGLVTGLVGLALIVWHRIGAEIYAPDALLGAVAVIVSALL
                     YAATSILLRARATRDHAAWIVCVQPMLPALVLMPSAVPGWTPVAQMDLMIFVGIGLLG
                     TIGHFVLAHAFARAEAARLAPLHYVTLVWGVLIGYVFYSEIPSLMVLAGAALIIAGAL
                     ATSWK"
     gene            complement(1963..3423)
                     /gene="hydA"
                     /locus_tag="PK264_23280"
     CDS             complement(1963..3423)
                     /gene="hydA"
                     /locus_tag="PK264_23280"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRD78824.1"
                     /translation="MSYDIVIRGGMVATAGETFAADVAIAGETVVAIGRDLPKGAREI
                     DAKGLIVMPGGVDGHAHIEQMSGMGLMGADNFESGTTSAAFGGTTTVVCFAAQHQGKS
                     LTKIVEDYHALARKGAVIDYAMHMILADPRPEVLAEELPPLVKAGHASIKVFMTYEKT
                     KIDDEQLLDVLTAARANNAFVCVHAENNGMLNWMGRRLVDRGYVAPKYHAVYHPRAAE
                     VEAIHRLIAFAELIDQPVIVYHVSTAEGAAAIREARGRGVKVFGETCTQYLFLTAADL
                     DQPGIEGIKWICSPPLRTKEDQEALWAALERGDLQFISSDHAPYRPDASGKLKVGPKP
                     TFKQVANGMPGLELRLPLLFDAMVSKGRYDLQKFVAWTATEPAKMYGLHPKKGTIAVG
                     SDADIAIWDPKKSVTIRDEDVHDRTGYTPYVGRRIKGWPVTVLRRGEMVIEKGRALAK
                     PGSGKFLPRAAGAAAVPTGRLSPEFDIKRNFGAKLY"
     assembly_gap    3433..3461
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3599..>3817
                     /locus_tag="PK264_23285"
     CDS             3599..>3817
                     /locus_tag="PK264_23285"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRD78825.1"
                     /translation="MKRFLVGAASLVLATVVGAVNAAASVKVHVDIGSQRMQVYIDGR
                     LKHEWAVSTGRDGYETPTGSYRPQRLERE"
     assembly_gap    3818..3961
                     /estimated_length=144
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          701 a         1302 c         1354 g          647 t
ORIGIN      
        1 cctcgttgag gggcaccgca tgggccgccg tcagcacctg aagcgaagtg atgcccggga
       61 cgacatgcac ggagagcggc agcacgcgcg cgttgggcga gcacgcaccg gcgccgggca
      121 ggtcgagatt gcccttgagg gcggccttgc cgacgggcgc cacgtccgtg tcctggtcgg
      181 ccgagttgtt gcgctcgtag acgcagccat tggaggagcg gccgttcgag acggcgccgg
      241 cgaggccgcc cgcgttgacg cctgccgaga acggcgccag cgccacgcgc acctcgttcg
      301 tcgtgccggc atccggcatc aggatgtcga ggagatcgac gacggccacc ttcaggtctt
      361 cgagcttgcg gccgtgcatg gagcccgtga cgtcgagctg gaggccgacc tcgacgtcct
      421 tgcgatcata gatggcgacc gccgagcgag gaaaagagat cttgctgatg ccggcgatgt
      481 tggcgaacag cgtcggcaca tcggcgtcga cgtcgagggc gatcgagttg ttgccgcggt
      541 tgatggcgac gcgcagctcg gagacgttgg cgtagttggc gccggtgccg tccatgttga
      601 tcgcgaagta gcgcttggcg aggtcgtcga tctcgccgtc cgtgaggttc tggtcgcgca
      661 tgcccttcgc cgcggcgagc gcggcgctgt cgagcgcgtc ggagatcttc tggttcgcgt
      721 gcatggcgcg gccgacgtcg atggcaagtc ccgtcaccat gacgaggaag aacgcgcaga
      781 tcgcgaagat catggcgatc gtaccgtcct cgctgcgccg gaacgagacg gcgagggtct
      841 gagaggactg cgacatgggt gactcccgaa gcaaacacgg cggtttgcct tcagggaatg
      901 caacgccaat gccggttcga gccctggtcc ggacaaggaa aacggcccgt taagcagcgt
      961 gccgcctcag ggcgaaacgg ctgaaaacga ccctatttcc agctcgtcgc cagcgccccg
     1021 gcgatgatga gcgcggcgcc ggcgagcacc atgagcgagg gaatctcact atagaacacg
     1081 tagccgatga gcacgcccca aacgagtgtc acataatgga gcggcgcaag ccgcgccgcc
     1141 tccgcccgcg cgaacgcgtg ggccagcacg aagtggccga tcgtgcccag gaggccgatg
     1201 ccgacgaaga tcatgagatc catctgcgca accggtgtcc agccgggaac ggcgctcggc
     1261 attagcacga gtgcgggcag catgggctgc acgcacacga tccaggccgc gtgatcccgc
     1321 gtcgcccttg cccggagcag gatcgacgtc gcggcataga gcagggcgga cacgatgacg
     1381 gccaccgcgc cgagcagggc gtccggtgca tagatctcgg cgccgatccg gtgccacacg
     1441 atcagcgcaa gcccgacaag tcccgtcacg agccccgccg cgatccgccc ttccagctgc
     1501 tcaccaagca cgatgacacc gagcagcacc atgaagattg gcgcgatgaa cgagagcgcg
     1561 aacacctccg caagcggcag ctggccgagc gcatagaaga agagcgtcgc ggttgcgacc
     1621 gaaagggccg agcgcaccgc attggccttc acggtctcga gcgtcggcca gccaggtcgc
     1681 agcactgcca caagagccgc cgcccacacg aagccgaagc cgaaccgcag ccccgcgatc
     1741 tgcagcgtcg ggtagcgctg cgcgaggccc ttgatgatgc cgtccatgat ggtgagcagg
     1801 ccctcggcga gcacgagcag cagggcagcg tgggtcatgg acggtcctgc gccttggcga
     1861 gctcgggtat cgttcgcgga acgcggctgc ggaaacgacg cgagccagcg cttcctcgat
     1921 cgaggattcg ccgcgtcaag cgctatcaca cgtcaggaga gttcaataca gcttggcgcc
     1981 gaaattcctc ttgatatcga actcgggcga caggcggccg gtcggcaccg cggccgcacc
     2041 cgccgctcgg ggaaggaact tgccggaacc cggtttggcg agcgcgcgcc ccttctcgat
     2101 caccatctcg ccgcggcgga gcaccgttac cggccagccc ttgatgcgtc ggccgacgta
     2161 gggcgtgtag ccggtccgat cgtgcacgtc ctcgtcgcgg atcgtgacac tctttttcgg
     2221 gtcccagatg gcgatgtcgg cgtcgctgcc gactgcaatc gtgcccttct tcgggtgcag
     2281 gccatacatc ttcgcgggct cggtcgcggt ccaggcgacg aatttctgaa ggtcgtagcg
     2341 gcccttgctc accatggcat cgaagagcag cggcagacgc agctcgagcc ccggcatgcc
     2401 gttcgccacc tgcttgaagg tcggcttcgg tcccactttc agcttgccgc tcgcgtccgg
     2461 ccgatagggc gcgtggtcgg aggagatgaa ctgcaggtcg ccgcgttcga gtgccgccca
     2521 gagcgcctcc tgatcctcct tcgtgcgcag cggcggcgag cagatccatt tgatcccttc
     2581 gatgcccggc tgatcgaggt ccgccgccgt caggaagaga tactgcgtgc acgtctcacc
     2641 gaacaccttg acgccgcgtc cgcgcgcctc acggatggcg gcggcgccct cggccgtcga
     2701 gacgtgatag acgattacag gctggtcgat gagctctgcg aaggcgatca ggcgatggat
     2761 cgcttccacc tcggccgcgc gcgggtgata gaccgcgtga tacttgggcg ccacatagcc
     2821 tcgatcgacg agccggcggc ccatccagtt gagcatgccg ttgttctcgg catgcacgca
     2881 gacgaaggcg ttgttggcgc gcgcggccgt gagcacgtcg agcagctgct cgtcgtcgat
     2941 cttcgtcttc tcgtaggtca tgaagacctt gatcgaggcg tgcccggcct tgacgagcgg
     3001 cggcagctcc tccgcgagca cctcggggcg cggatcggcg aggatcatgt gcatcgcgta
     3061 atcgatcacg gcgcccttac gggcgagcgc atgatagtct tcgacgatct tggtgaggct
     3121 cttcccctgg tgctgagcgg cgaagcagac gaccgtcgtc gtgcccccga aggccgccga
     3181 tgtcgtgccg ctctcgaagt tgtcggcgcc catcaggccc atgcccgaca tctgctcgat
     3241 gtgcgcgtga ccgtcgacgc cgccgggcat aacgatcaag cccttggcat cgatctctcg
     3301 ggcgcccttc ggcaggtcgc gcccgatcgc gaccaccgtc tcgccggcga tcgccacatc
     3361 ggccgcgaaa gtttctccag cggtcgcgac catgccgccg cgaatgacga tgtcgtagct
     3421 catggaggca ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggctttggc cgataacata
     3481 gctcgcacgc gggcgttatc caccacggac ttgcggtcga ggcgaagctc accttgcttg
     3541 ctcactcagt cgacgaacaa tctggtgtgg cggtccaacc cacaattcag agggcatcat
     3601 gaagcggttt ttggtcgggg cggcgagcct cgtgctcgcc accgtcgtcg gcgcggtgaa
     3661 tgcagcggca tcggtcaagg tgcacgtcga tatcggctca cagcggatgc aggtctacat
     3721 cgacggccgc ctgaagcacg agtgggccgt atcgaccggc cgcgacggct acgagacgcc
     3781 gaccggcagc taccgcccgc agcgcctcga gcgtgagnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 naatctcgtc gagcgctacg ggcggaattc gacacgcatc tcgatcgatg attgagtgac
     4021 gagcctcagt taggaccggc tgactacgcg attgcgtagt cggcgcgcag cgcaacgtca
     4081 gagatgtggc cagcccgcgc cggccggcgc gcggtgacgc gtgtcggcgc gaagacaacg
     4141 tccgagatct ggccagcccg cggcgggcgg cgcgcgg
//
LOCUS       DAORNH010000360         4171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15931_length_4171_cov_2.657677, whole genome shotgun sequence.
ACCESSION   DAORNH010000360 DAORNH010000000
VERSION     DAORNH010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15931_length_4171_cov_2.657677"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>375
                     /locus_tag="PK264_23290"
     CDS             <1..>375
                     /locus_tag="PK264_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78826.1"
                     /translation="EDITIDYGPPQLLGRVFLQAETQARLAGVRLSFAPVRTLAEVNR
                     ANRDSWKALLPTFDVAYGGISDDNAYCIIGRDASGDIVATQGARFFSWTDTSFHEELE
                     SLRLLYGDPARMRGADESCTVTA"
     assembly_gap    376..385
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <386..793
                     /locus_tag="PK264_23295"
     CDS             <386..793
                     /locus_tag="PK264_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78827.1"
                     /translation="AAASSSPTRNTTVTAPSARTLRGRVVYSGGVWYRPDYRKKGLAS
                     CITRLSKGLAFTRWYSDVTLSLMTDDTVVGGTWKRTGYPNLEWDVTIRNCPLGTIRCA
                     IIWMRMEELLADLEGYLAGVSPEVDRIVQDRAA"
     gene            complement(740..1588)
                     /locus_tag="PK264_23300"
     CDS             complement(740..1588)
                     /locus_tag="PK264_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78828.1"
                     /translation="MVLIDDQGEFWDGASRQLRTAFDSPFSGGEFSSYAVRNLGFVAV
                     DQFGSSCQIRLRPAMVSSKAVAALVEWLHKRSFQRVSAAWFTTDWAYEIFPSLREALG
                     RLASVIEEARTPASSDFKTKAIGPNDVKQANALVALMDRWQSLSGEGRFTELEWLIRQ
                     SLGKRYAFVERDDGSSKLVFRELGSGIIDHHQDWRATALGQPLEALPDRAYGQWVNAS
                     FEASLAAGAPRIEAVDAIVRWPHRGRTRLRYKRVIVPLAVSSGRPALLGGSILDDSID
                     LRADAR"
     gene            complement(1792..>2736)
                     /locus_tag="PK264_23305"
     CDS             complement(1792..>2736)
                     /locus_tag="PK264_23305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRD78829.1"
                     /translation="LECVVPEIEQASGKKLGLHFGVCFNPEFLREGVAVADFYAPPKT
                     VIGSSDKRSEEILSRIYKSVDEKLIFTSIEAAEMVKYADNVWHATKVAFGNEIGRICK
                     SLDIDSHDVMNIFVKDLKLNLSPYYLKPGFAFGGSCLPKEVRAVSHLAGEKGVSVPLI
                     DSLSVSNDHHIDEAVRLLEPFLGKRIGFLGVTFKSNTDDLRESPTLELMARLLDAGAN
                     VQAYDPNLHFGPHLQGQIDYVRHACPSQARLMDKLDGLALATADALIDASDVVVVSHA
                     TDEFRAAVAKRGKDVHVLDLVRLFPTRPDEATYQGIAW"
     assembly_gap    2737..2746
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2747..3214)
                     /locus_tag="PK264_23310"
     CDS             complement(<2747..3214)
                     /locus_tag="PK264_23310"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose dehydrogenase"
                     /protein_id="HRD78830.1"
                     /translation="MNMPQRGIDPASRAAERGLSVVTSEKPSISVIGLGYVGAVSMAC
                     LAHLGFRMVGVDVSAAKVKSVAEGRSPIVEARLEELLTEGVERGLISATQNLVGAVLE
                     TDVTFMSVGTPTSADGGCDFTYVRQASRAIGQALAMKSAFHVVVMRCSVPPGTT"
     gene            complement(3394..>4171)
                     /locus_tag="PK264_23315"
     CDS             complement(3394..>4171)
                     /locus_tag="PK264_23315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRD78831.1"
                     /translation="TLTGRCSMFRAEHIVSHEFIRLQEADFLDHWLWGKFRFLSGDDK
                     STWYTLLKYGVKMTYVPDASGTTIEVVDGSGTKRMVENLRRWSGNMLRNGYRAIMLGP
                     TRMPFFIWWCCVDQRLAMWTMLFSPMLAFAGLLKIGSGFLFAYIVYIAVTRMLLSLVL
                     FTYARQVDLNYVWCLYTNQLLNAAVKIYMMWRLAKQKWANRGNQKAGFSGGSLLAAAR
                     EMMASYLTLLSFAALFLCVAIYTQLLNMPSWSLVGHILFG"
BASE COUNT          776 a         1364 c         1325 g          686 t
ORIGIN      
        1 gaagacatca ccatcgacta cggcccgccg cagctgctcg gccgggtctt cctgcaggcc
       61 gagacccagg cgcggctcgc cggggtccgc ctctcatttg ccccggtccg cacgctggcc
      121 gaggtcaacc gcgccaaccg cgacagctgg aaagcgctgc tgccgacgtt cgacgtcgcc
      181 tatggcggca tcagcgacga caatgcctat tgcatcatcg gccgggatgc ctcgggcgac
      241 atcgttgcga cgcaaggcgc ccgcttcttc tcgtggaccg acacgagttt ccatgaggag
      301 ctcgagagcc tccgcctcct ttacggcgat ccggcgcgca tgcgaggcgc tgatgaaagc
      361 tgcacggtga cggcgnnnnn nnnnngctgc ggcctcgtcg tcgccgacgc gcaacacgac
      421 ggtgacggcg ccctctgccc gcacgctgcg cggtcgcgtc gtctattcgg gtggtgtgtg
      481 gtaccggccg gactaccgca agaaggggct cgcctcctgc atcacgcggc tctcgaaggg
      541 cctcgccttc acgcgctggt acagcgacgt cacgctgagc ctgatgaccg acgataccgt
      601 cgtcggcggc acctggaagc gcacgggcta tcccaacctc gagtgggacg tgaccatccg
      661 caactgcccg ctcggcacca tccgctgtgc catcatctgg atgcggatgg aggagttgct
      721 cgccgatctc gagggctacc tagcgggcgt cagcccggag gtcgatcgaa tcgtccagga
      781 tcgagccgcc tagcagcgcc ggtctgccgc tcgataccgc gagaggtacg atcacccgct
      841 tgtagcggag ccgcgtgcgc ccgcgatgcg gccagcgcac gatcgcgtca actgcctcga
      901 tgcgcggtgc tcccgcggcg agcgaggcct cgaacgaggc attgacccac tggccgtagg
      961 ctcggtccgg cagcgcttcg agcggctgtc cgagggccgt cgcccgccaa tcctgatggt
     1021 gatcgatgat gcccgagcca agctcgcgga agacgagctt agagcttcca tcgtcacgct
     1081 cgacgaaagc atagcgcttg ccgagcgact ggcggatgag ccactcgagc tcggtgaagc
     1141 gcccctcgcc cgaaagcgac tgccagcgat ccatcagcgc cacgagcgca ttcgcctgct
     1201 tgacgtcatt cggaccgatg gccttcgtct tgaaatccga cgaggcgggt gtgcgcgcct
     1261 cctcgatgac gctcgcgagg cggccgaggg cctcacgcag cgacgggaag atctcatagg
     1321 cccagtccgt cgtgaaccag gccgccgaca cgcgctggaa cgagcgcttg tgcagccact
     1381 cgacgagcgc cgcaacggcc tttgaggata ccatcgccgg cctgagccgg atctggcacg
     1441 acgagccgaa ctggtcgacc gcgacgaagc cgaggttgcg gaccgcgtag ctcgagaact
     1501 cgccccccga gaacgggctg tcgaatgcgg tccggagctg gcgcgaggcg ccatcccaga
     1561 actcaccctg atcgtcgatc aagaccatcg gcacgcgcgt acaagcccca gccaatccga
     1621 gcacgatccg ccaaggatgc acgacaagag ttagaaatca cttgcgcggc cgcgcggatt
     1681 gagccgcgca ttcaattgaa actgaaccat ttttgcgcag gcacgcacta acgaactgtg
     1741 cctcgcccgg cttgcccggg cggaggcgcc aacgtcgcga tgtgtcgatc cttaccaggc
     1801 gatgccctga taggtcgcct cgtccggtcg cgtcgggaag aggcggacga ggtcgagcac
     1861 atgcacatcc ttgccgcgct tcgcaaccgc ggcgcgaaac tcatccgtcg catggctgac
     1921 cacgaccacg tccgatgcgt cgatcagtgc atcagccgtc gccagcgcaa gcccatcgag
     1981 cttgtccatg agtcgcgcct gactcgggca ggcgtggcgc acgtagtcga tctggccttg
     2041 cagatgcggc ccgaaatgca ggttcggatc ataggcctgc acattcgcac ccgcatcgag
     2101 gagccgggcc atcagctcga gcgtcgggct ctcgcggagg tcgtcggtgt tcgacttgaa
     2161 ggtcacgccg aggaagccga tgcgcttgcc gaggaacggc tcgagcaggc ggacggcctc
     2221 gtcgatgtga tggtcattcg acaccgagag gctgtcgatc agcggcaccg acacgccctt
     2281 ctcacccgcg agatgggaga ccgcgcgcac ctccttcggc aggcacgagc cgccaaacgc
     2341 gaagcccggc ttcaggtagt agggcgagag gttgagcttc aggtccttga cgaagatgtt
     2401 catgacgtcg tggctgtcga tatcgagcga cttgcagatg cgcccgatct cgttgccgaa
     2461 ggcgaccttc gtcgcgtgcc agacgttgtc ggcatacttc accatctccg ccgcctcgat
     2521 ggaggtaaaa atgagcttct cgtccaccga cttgtagatg cgcgagagga tctcctccga
     2581 gcgcttgtcc gaggagccga ttacggtttt cggcggcgca tagaaatcgg cgacggcgac
     2641 gccctcgcgc aggaactccg ggttgaagca gacgccgaaa tggagcccga gcttcttgcc
     2701 cgaggcctgc tcgatctctg ggacgacgca ctctagnnnn nnnnnncgtt gtgccgggcg
     2761 gaaccgagca gcgcatgacg acgacatgga aggcgctctt catggccagt gcctggccga
     2821 tcgcccggct cgcctgacgc acataggtga aatcgcagcc gccgtcggca gaggtcggcg
     2881 tgccgaccga catgaaggtg acgtcggtct cgagcacggc gccgacaagg ttctgcgtcg
     2941 cgctgatgag gccgcgctcg acgccctccg tgagcagctc ctcgagccgc gcctcgacga
     3001 tcggcgagcg cccctcggcc accgacttca ccttcgcggc ggaaacgtcg acgccaacca
     3061 tgcggaaccc gaggtgcgca aggcacgcca tcgagacggc cccgacatag ccgagaccga
     3121 tcacgcttat cgaaggcttc tcggaggtca cgacgctgag cccgcgctcg gcggctcggc
     3181 tcgcggggtc aattccgcgc tgcggcatgt tcatgttgtc tgctcccact catcacccct
     3241 gtcgctggtg ggagacgacc gcgcgcttca gtccgcatcg gctcgccgct cttccctcca
     3301 gcccggagag catcgcagag acgcgtggca tttgactgaa gcgagacact acaattcgaa
     3361 gcaatttccg cgcattaacc tcggtgcgtg acgtcagccg aacaggatgt ggccgacgag
     3421 gctccagctc ggcatgttga gcagctgtgt atagattgcg acgcacagga acagcgcggc
     3481 gaaggagagc agcgtcagat acgacgccat catctcgcgg gccgcagcca agaggctgcc
     3541 gccggagaag ccggctttct ggttgccgcg gttcgcccac ttctgtttgg cgaggcgcca
     3601 catcatgtag atctttaccg ccgcgttgag cagctggttc gtatagaggc accacacata
     3661 gttgaggtcg acctgacgcg cgtaggtgaa gagcacgagc gacagcagca tccgcgtgac
     3721 cgcgatgtag acgatgtagg cgaacaggaa gccggagccg atcttcagga gacccgcgaa
     3781 agccagcatc gggctgaaca gcatcgtcca catcgcaagg cgctggtcga cgcaacacca
     3841 ccagatgaag aagggcattc gcgtcggccc gagcatgatg gcgcgatagc cgttgcgcag
     3901 catattgccg gaccagcgtc gcaggttctc gaccatgcgc ttcgtgccgg agccgtcgac
     3961 gacctcgatc gtggtgcccg acgcgtccgg cacataggtc attttcacgc cgtacttgag
     4021 cagcgtgtac caggtgctct tgtcgtcgcc cgagaggaag cggaatttgc cccagagcca
     4081 atgatcgagg aaatcggcct cctgcagacg gatgaactcg tgcgagacga tgtgctcggc
     4141 gcggaacatc gagcagcggc ccgtcaacgt c
//
LOCUS       DAORNH010000361         4171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15932_length_4171_cov_2.422983, whole genome shotgun sequence.
ACCESSION   DAORNH010000361 DAORNH010000000
VERSION     DAORNH010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15932_length_4171_cov_2.422983"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK264_23320"
     CDS             complement(<1..749)
                     /locus_tag="PK264_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HRD78832.1"
                     /translation="MSLRSRGLWASFAAVALLSVALAPHLTRAAGETRSISLYNIHTK
                     EQATVVYKRDGRFVADGVEKASWLLRDWRRNEATKMDPELIDLLWEIHAELGSQQPIH
                     IISGFRSRTTNDMLRRTVGGQASESQHIHGKAADVHFPDIPLKSLRYSALIRERGGVG
                     YYPTSAIPFVHVDTSRVRHWPRLPRHELALLFPSGKSQHMPADGGPITAEDVRVAQSR
                     YRDLAIQVAAFHGDRKRPGGILVASARPPAP"
     gene            1072..2514
                     /locus_tag="PK264_23325"
     CDS             1072..2514
                     /locus_tag="PK264_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRD78833.1"
                     /translation="MTTTVIRNITWLVAYDQARGTHMYVTDGDIAFADGAITHVGGRY
                     AGAAVREVDGRGFLVMPGLVNIHSHPASEPMNKGWNDEIGSPKLYNSSLYEIMPIFRP
                     DPDGVPAAATVAYAELLQSGVTTLVDLSVAWDGWLDLAAQSGLRVVLAPMYRSARWYT
                     ANGHVVAYEWDEAAGLRAMEAAHALVDRAVAHPTGRLSGMIAPSQIDTCSPELIRQSL
                     AAAKARGLKLQIHAAQSVVEVHEITRRHGLLPIAWLDSLGALTPDTIIGHGIFLDHHG
                     SVHWPKSDEIGRLIATGTSVAHCPTVFLRRGIALTTFGRYVRRGVNVGIGTDTFPHNM
                     IEEIRNALYTSRIVAEDPFDVTTAEGLHAATLGGAKALGRSDIGRLSVGAKADFVMVD
                     TRQQNMRPVRDPLRSLIYAADRAVRHVYVGGEQVVVDGRVTTLDQAAAAERVEAAQRR
                     AEPKVAGLDWAHRSHFELSPLALEVKGRNV"
     gene            2766..>4171
                     /locus_tag="PK264_23330"
     CDS             2766..>4171
                     /locus_tag="PK264_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRD78834.1"
                     /translation="MAKKVNLRSQAWFSRQDKMGFLYRSWLKNKGIPHDQFDGRPVIG
                     ICNTWSELTPCNSHFRVIADNVRMGILEAGGFPLEFPVMSLGETLLRPTAMLYRNLAS
                     MDVEESIRANPLDGVVLLVGCDKTTPALLMGASSVDVPAIAVSGGPMLRGPYRGSYIG
                     SGTNTFSLSEDLRAGKITHEEFNEAESCMHRSHGHCMVMGTASTMASMVEALGIGMPG
                     NAAIPAVDGRRMVLAREAGRRIVQMVKDDIQMSKILTRQAFENAIRVNAAIGGSTNAV
                     IHLIAVARRLGVELRLEDWDEFGTGVHTLVNIKPSGDYLMEDFFEAGGIPAVIRELGE
                     NGKLNKSAMTVNGKTIWENNKAAPCWNRKVIHEWRSPFKHNTGIAVLRGNLAPEGAII
                     KPSAATPKLLKHKGRAVVFESYEDMHHAVDDPKLDIDENCVMVLKGAGPKGYPGMAEV
                     GNMPLPQKILKKGITDMVR"
BASE COUNT          762 a         1404 c         1363 g          642 t
ORIGIN      
        1 atcggtgccg ggggccttgc gctggcgacc aggatgccgc ccggacgctt ccgatcgcca
       61 tggaacgccg cgacctggat ggcgagatcg cgatagcggc tctgcgcgac acgcacgtcc
      121 tcggccgtga tcggaccgcc atcggccggc atgtgctgcg acttgccgga cgggaagagc
      181 agggcgagct cgtggcgcgg caggcgcggc cagtgacgca cgcgcgaggt atcgacatgg
      241 acgaacggaa tggccgaggt cggatagtag ccgacaccgc cgcgttcccg gatgagcgcc
      301 gaataacgca ggctcttcag cgggatatcc gggaaatgca cgtccgccgc cttgccgtgg
      361 atgtgctggc tctcgctcgc ctggccgccg acggtccgcc gcagcatgtc gttggtcgtg
      421 cgcgagcgga atccggagat gatgtggatc ggctgttgcg agccgagctc ggcatggatc
      481 tcccagagca gatcgatcag ctccggatcc atcttcgtcg cttcgttgcg acgccagtcg
      541 cgcagcagcc aactcgcctt ctcgacgcca tcggcgacga agcgcccgtc gcgcttgtag
      601 acgacggttg cctgctcctt ggtgtgaata ttgtagagcg agatcgagcg cgtctcaccg
      661 gcagcgcggg tgagatgggg cgcgagcgcg accgaaagga gcgcaacggc ggcaaagctt
      721 gcccaaaggc ctcttgaacg caacgacacg cgaatgaccc cgtccgaatg aacagtcccc
      781 agatcggcgc tctcccctgc gccgcccctt gttggcaact ccggcccgac cggttgctgc
      841 cgattgccgg gtcaaccttc atggttaatc cccgaccctt aagacttcgt aatgctgtca
      901 tgggacgcgg atgaggcgaa acggcggcga cacgtcatag ttgcagatgc gacgtctgcg
      961 agggtcgata gcgagcgagt agcgtccccc gggctgaagc ccgaacggaa gcgttgcaga
     1021 tggccgcgcg ccggtgtccc ataggtcaaa gcccaacttc agggagaggc catgaccacg
     1081 accgtcatcc gaaacatcac ctggctcgtt gcctacgatc aggcccgcgg cacgcacatg
     1141 tacgtgacgg acggcgacat tgccttcgcg gacggcgcga tcacgcatgt cggaggtcgc
     1201 tacgcaggcg ccgccgtacg cgaggtcgac ggccgcggct tcctcgtcat gcctggcctc
     1261 gtaaacatcc actcccaccc ggcgtctgag cccatgaaca agggctggaa cgacgagatc
     1321 ggctcgccga agctctacaa ctcctccctc tacgagatca tgccgatctt ccgccccgac
     1381 ccggacggcg tgccggccgc cgcgaccgtc gcctatgccg agctcctgca atcgggcgtc
     1441 acgaccctcg tcgacctttc ggttgcctgg gacggctggc tggacctcgc ggcccagagc
     1501 ggcctccggg tcgtgctcgc gcccatgtac cgctcggcgc gctggtacac ggcgaacggc
     1561 cacgtcgtcg cctacgagtg ggacgaggcg gccggtctca gggcgatgga agccgcgcac
     1621 gcgctcgtcg accgggctgt ggcgcaccca accggccgcc tttccggcat gatcgcgccg
     1681 tcgcagatcg atacctgctc gcccgagctc attcgccaaa gcctcgccgc cgccaaggcg
     1741 cgcggcctca agctgcagat ccacgcggcg cagtccgtcg tcgaggtgca cgagatcacg
     1801 cgccgccacg gacttctgcc gatcgcctgg ctcgacagcc tcggcgcgct gacccccgac
     1861 acgatcatcg gccacggcat cttcctcgac catcacggca gcgtgcactg gccgaagagc
     1921 gacgagatcg gccggctgat cgcgaccggc accagcgtcg cgcactgccc gacggtgttc
     1981 ctgcgccgcg gcatcgcgct caccaccttc gggcggtatg tgcggcgcgg tgtcaatgtc
     2041 ggcatcggca cggacacctt cccgcacaac atgatcgagg agatccgaaa cgccctctat
     2101 acctcgcgca tcgtggccga ggatccattc gacgtgacga cggccgaggg cctccacgcc
     2161 gcgaccctcg gcggtgccaa ggcgctcggg cggagcgata tcggtcgcct ctcagtcggc
     2221 gccaaggccg acttcgtcat ggtcgatacg cggcaacaga acatgcgccc cgtccgcgat
     2281 cctctgcgaa gcctcatcta cgcggccgat cgcgcggtac ggcatgtgta cgtcggcggc
     2341 gagcaggtgg tcgtcgacgg ccgcgtgacg acgctcgacc aggccgctgc ggccgaacgc
     2401 gtcgaagccg cccagcgccg cgccgagccg aaggtcgccg gcctcgactg ggcccaccgc
     2461 tcccacttcg agctctcgcc gctcgccctc gaggtcaaag gccggaatgt gtgaggatca
     2521 ggaccgggct gatccgcaga gatcgagcgt cacagaaagc gtggggcaag gggatcggcg
     2581 ccaccgtcaa aggcgaaagg gggccggcga tgatgtggcc tcgtcgcgac agaggatgcg
     2641 ttgactgtcg acagcctgca ttgtccgacc atgccgagca gatacgttga cttggccagg
     2701 tgagcgcgcg aacaatgacg acgtgccgat ccggcaagcg acatcccgag acaacgagcg
     2761 cgacaatggc aaagaaggtc aacctgcgga gccaggcctg gttcagccgt caggacaaga
     2821 tgggcttcct ctatcgctcc tggctgaaga acaagggcat cccgcacgac cagttcgacg
     2881 gccggccagt catcggcatc tgcaatacct ggtcggagct gacgccctgc aattcgcatt
     2941 tccgggtgat cgccgacaac gtgcgcatgg gaattctcga ggccggcggc ttcccgctcg
     3001 agttcccggt gatgagcctc ggcgagactc tgctccgccc gacggcgatg ctctatcgca
     3061 acctcgcctc catggacgtc gaggagagca tccgggcgaa cccgctcgac ggcgtcgtcc
     3121 tgctcgtcgg ctgcgacaag accacgcccg cgctgctgat gggagcctcc agcgtcgatg
     3181 tcccggcgat cgcggtctcc ggcgggccga tgcttcgcgg gccttatcgc ggcagctaca
     3241 tcggctcggg caccaacacg ttctcactct cggaggatct gcgcgccggc aagatcacgc
     3301 atgaggagtt caacgaggct gaaagctgca tgcatcggag ccacggccac tgcatggtga
     3361 tgggcacggc ttcgacgatg gcctcgatgg tggaggcgct gggcatcggc atgcccggaa
     3421 acgccgccat tcccgcggtc gacggccgcc gcatggtgct cgcgcgagaa gcaggccggc
     3481 gcatcgtgca gatggtgaaa gacgacatcc agatgtcgaa gatcctcacc cgccaggcct
     3541 tcgagaacgc gatccgtgtc aatgccgcca tcggcggctc cacgaacgcg gttatccatc
     3601 tcatcgcggt ggcgcggcgc ctcggcgtcg agctccgcct cgaggactgg gacgagttcg
     3661 gcacgggcgt gcacacgctc gtcaacatca agccgtcagg cgactacctg atggaggact
     3721 tcttcgaggc gggcggcatc ccggccgtca tccgcgagct cggcgaaaac ggcaagctca
     3781 acaagagcgc gatgaccgtc aacgggaaga ccatctggga gaacaacaag gcggcaccct
     3841 gctggaaccg caaggtgatc cacgagtggc gctcgccctt caagcacaat acgggcatcg
     3901 ccgtgctgcg cgggaacctc gcgccggaag gtgccatcat caagccgtcg gcggcgacgc
     3961 cgaagctcct gaagcacaag ggccgcgccg tcgtcttcga gagctacgag gacatgcacc
     4021 acgcggtcga cgacccgaag ctcgacatcg acgagaattg cgtgatggtg ctgaagggcg
     4081 cgggcccaaa gggctatccg ggcatggcgg aggtcggcaa catgccgctg ccgcagaaga
     4141 tcctgaagaa gggcatcacc gacatggtgc g
//
LOCUS       DAORNH010000362         4170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_15939_length_4170_cov_2.361604, whole genome shotgun sequence.
ACCESSION   DAORNH010000362 DAORNH010000000
VERSION     DAORNH010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15939_length_4170_cov_2.361604"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK264_23335"
     CDS             <1..227
                     /locus_tag="PK264_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRD78835.1"
                     /translation="DVYGHTDSTGSDEHNFDLSQRRALSVANYLAGQGVDSRRFAVTG
                     FGKTRPIASNNTQAGRAQNRRVEIQLSPLT"
     assembly_gap    233..443
                     /estimated_length=211
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <444..729
                     /locus_tag="PK264_23340"
     CDS             <444..729
                     /locus_tag="PK264_23340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78836.1"
                     /translation="GRMRRMRGQEVAFADVGDDGSSGLGFGLDCGCFFARRVLLVATF
                     AARRAVAPVAAITAGRAVAAITAIAPVRGAIVAPVTAIRPVATIASVASL"
     assembly_gap    754..912
                     /estimated_length=159
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<913..3040)
                     /gene="pnp"
                     /locus_tag="PK264_23345"
     CDS             complement(<913..3040)
                     /gene="pnp"
                     /locus_tag="PK264_23345"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRD78837.1"
                     /translation="MFNTQRVEIEWGGRKLKLESGRMARQADAAVVAQYGETSVLATV
                     VFAKSARPGADFFPLTVNYQERTYAAGKIPGGFFKREGRPTEKETLTSRLIDRPIRPL
                     FVEGFKNETQVVVTVLSHDLENDPDIVGLVAASAALTLAGVPFLGPIGAARVGMIDGS
                     FVLNPMLDEMAKSSLDLVIAGTGDAVMMVESEAKELTEQQMLDAVMFGHRGFQPVLDA
                     IIKLAERSAKDAWNFQAPQKEKYLGKVRELVGDDLRQAFATPEKQKRNDMVEKAKEKV
                     VASLLAADASADDKVLVGDVFKAVEMEVVRGNIIRTGRRIDGRDLVSVRPITTEVHVL
                     PRTHGSALFTRGETQALCVATLGTGEDTQRIDALEGSHESHFMLHYNFPPYSVGETGR
                     MGSPGRREIGHGKLAWRAINPLLPSQEEFPYTIRIVSEITESNGSSSMASVCGGSLAL
                     MDAGVPLKRPVAGIAMGLIKEGNDYAVLSDIIGDEDHLGDMDFKVAGTEKGVTSLQMD
                     IKIAGITEEIMRKALEQAHAGRIHILGEMSKAIGSARTELGEHAPRIETIKIPVDKIR
                     EVIGSGGSVIREIVAESGAKVDIEDDGTVRVAAAKRESIEAALNRIRGITQEPEIGQI
                     YKGKVVKIMEFGAFVNFFGSKDGLVHISQLGPGRPRTVGEVVQEGQQVFVKLLGFDDR
                     GKVRLSMKIVDQQTGQEIPEPPREARE"
     gene            complement(3185..3469)
                     /gene="rpsO"
                     /locus_tag="PK264_23350"
     CDS             complement(3185..3469)
                     /gene="rpsO"
                     /locus_tag="PK264_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRD78838.1"
                     /translation="MSLTAERKGQVIKDNAKKAGDTGSPEVQIALLTERINSLQDHFK
                     THKNDNHSRRGLLKMVSQRRRLLDYVRAADAARYQKIVENSDIEKLGIRR"
BASE COUNT          545 a         1254 c         1290 g          711 t
ORIGIN      
        1 tcgacgttta tggccacact gactcgaccg gctcggacga gcacaatttc gacctgtcgc
       61 agcgccgcgc gctgtcggtg gcgaactatc tcgccgggca gggcgtcgat tcgcgccgct
      121 tcgccgtcac cggcttcggc aagacgcggc cgatcgcctc gaacaacacc caggcgggca
      181 gggcgcagaa ccgccgcgtc gagatccagc tctcgccgct gacctgagcg gcnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnncggccgc atgcgccgga tgcgcgggca ggaggtggcg
      481 ttcgccgatg tcggcgacga cggcagcagt gggctgggtt tcggcctgga ttgcgggtgc
      541 ttcttcgcgc ggcgcgtgct gctcgtggcg acgttcgccg cgcggcgtgc ggtcgccccg
      601 gtcgccgcga tcactgcggg gcgggcggtc gccgcgatca ccgcgatcgc cccggtccga
      661 ggcgcgatag tcgccccggt caccgcgatc cggccggtcg ccacgatcgc ctcggtcgcc
      721 tcgctctgag cgctcgtacc ggtcaccccg atcnnnnnnn nnnnnnnnnn nnnnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nncctcgcga gcctcgcgcg gcggctcggg gatctcctgg ccggtctgct
      961 ggtcgacgat cttcatcgac aggcggacct tgccgcggtc gtcgaaaccc aggagcttta
     1021 cgaagacctg ctggccctcc tgcacgacct cgccgacggt gcgcggccgg cccggcccga
     1081 gctgcgagat gtgcacgaga ccatccttcg agccgaagaa gttcacgaag gcaccgaact
     1141 ccatgatctt cacgaccttg cccttgtaga tctggccgat ctcgggctct tgcgtgatgc
     1201 cgcggatgcg attgagggct gcctcgatgc tttcgcgctt ggccgcggcg acgcgcacgg
     1261 tgccgtcgtc ctcgatgtcg accttggcgc cgctctcggc gacgatctcg cggatgaccg
     1321 agccacccga tccgatcact tcgcggatct tgtcgaccgg gatcttgatc gtctcgatgc
     1381 gcggcgcgtg ctcgccgagt tcggtgcggg cggagccaat ggccttcgac atttcgccga
     1441 ggatgtggat gcggccggcg tgcgcctgct ccagcgcctt gcgcatgatt tcctcggtga
     1501 tgccggcgat cttgatgtcc atctgcagcg atgtgacacc tttctccgta ccggccacct
     1561 tgaagtccat gtcgccgagg tggtcctcgt cgccgatgat gtcggagagg acggcgtagt
     1621 cgttgccctc cttgatgagg cccatggcga tgccggcgac gggtcgcttc agcggtacgc
     1681 ccgcatccat gagcgcgagt gagccgccgc acacactcgc catcgacgag gagccgttgc
     1741 tctcggtgat ctcggacacg atgcggatcg tgtacgggaa ctcctcctgc gaggggagca
     1801 gcgggttgat cgcgcgccag gcaagcttgc cgtggccgat ctcgcggcgc ccgggtgagc
     1861 ccatgcggcc tgtctcgccg accgagtagg gcgggaaatt gtagtgcagc atgaagtgcg
     1921 attcatggct gccctcgagc gcgtcgatgc gctgcgtgtc ttcgccggtg ccgagtgtcg
     1981 ccacgcacag cgcctgcgtc tcgccacgcg tgaagagggc ggagccgtgg gtgcgcggca
     2041 gcacgtgcac ctcggtcgtg atggggcgga cggagacgag atcgcgcccg tcgatgcgcc
     2101 ggccggtgcg gatgatgttg ccgcgcacga cctccatctc gacggccttg aagacgtcgc
     2161 cgacgagcac cttgtcgtcc gcgctcgcgt ccgccgcgag cagcgatgcg acaaccttct
     2221 ccttggcctt ctcgaccatg tcgttgcgct tctgcttctc gggcgtggcg aaggcctggc
     2281 ggagatcgtc gccgacgagc tcgcgcacct tgccgaggta cttttccttt tgcggcgcct
     2341 ggaagttcca ggcgtccttc gccgagcgct cggcgagctt gatgatggcg tcgagcaccg
     2401 gctggaagcc gcggtggccg aacatcacgg cgtcgagcat ctgctgctcg gtgagctcct
     2461 tcgcctcgga ttcgaccatc atcacggcat cgcccgtgcc ggcgatgacg aggtcgaggc
     2521 tcgatttcgc catctcgtcg agcatcggat tgaggacgaa gctgccgtcg atcatgccga
     2581 cgcgcgccgc gccgatcgga ccgaggaaag ggacgcctgc gagcgtcaac gccgcggagg
     2641 ctgccaccaa gccgacgatg tcggggtcat tctcgaggtc gtgcgaaagc acggtgacga
     2701 cgacctgcgt ctcgttcttg aagccctcaa cgaagagggg ccggatcggc cggtcgatca
     2761 ggcgcgaggt cagcgtctct ttttcggtcg ggcgtccctc gcgcttgaag aagccgcctg
     2821 ggatctttcc ggcggcatag gtccgctcct ggtagttgac ggtgagcggg aagaagtcgg
     2881 cgccggggcg cgcgctcttc gcgaagacga cggtcgcgag gacgctggtc tcgccgtact
     2941 gggcgaccac ggcggcatca gcctgccggg ccatgcggcc gctctcgagc ttgagcttgc
     3001 gcccgcccca ctcgatctcg acgcgttgcg tgttgaacat gattgctttt ctctttcgat
     3061 ctctggatcg ttgcggttcg gaaatggcca aaaggccggt catctccgca cgggagccgg
     3121 cgcgctatcc atgtcgactg gaatccgggc gccgcccctc gaaggggagc tcagatgagc
     3181 tccgtcagcg cctgatgccg agcttctcga tgtccgagtt ctcgacgatc ttctggtacc
     3241 gggccgcgtc cgctgcgcgc acatagtcga gcaggcggcg gcgctggctg accatcttga
     3301 gaaggccgcg gcgcgagtgg ttgtcgttct tgtgagtctt gaagtggtcc tgcagcgagt
     3361 tgatgcgctc ggtcaacaga gcgatctgca cttccggcga cccggtgtcg ccggccttct
     3421 tggcgttgtc cttgatgacc tggcctttgc gttcggcagt cagcgacatc gaatatcctt
     3481 ccgttgaaac gaggtgtgct cttggatccg gcgtgccaaa gcggcaatcg actgccgtga
     3541 acatgtggga tgccacatgg gcgtaccggg atgcggccgg agccgggatg tcgtccagca
     3601 gggtcgcttt tggcgtgtgt cggctgcggg ccgcatgcac gccgagggcc ttatacacgt
     3661 ttcgggggcc cgtgcgagga gaaactgcaa cagggtgcag ccgggaaccg ccatccctcg
     3721 accgagcgca tatgcctccc acgtcgtccc gtcgaccccc tgcggccttc gcttacgatt
     3781 cggagcccgc cccgtgcccc gacgcgggag cccactcgcg tgagcgtcgc ttcgcgaccc
     3841 ggctaccgcc cgcgcccgcc tgggggaagc ttcccccgaa ccccctactc gatttgtgac
     3901 caagtttagc gtcgggaggc gccaagccgg ggcatgacgt gcggcgcaag ccatttgaga
     3961 tgaaagaggc taggtctggg gcggcctcag cccaaggcgg gtctgggcgg cagcccagtc
     4021 gcgaagcgac atcccacgcc agcgggcctc gtattcagcg cccgttcgtg tacagtttcc
     4081 ccgttttccg acggaggccc catgctgcgc aacctgttcg cggttgtcgc cgcgcttgcc
     4141 gccgcctgcg cgggcgaggg cgagtagctg
//
LOCUS       DAORNH010000363         4105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_16289_length_4105_cov_2.588148, whole genome shotgun sequence.
ACCESSION   DAORNH010000363 DAORNH010000000
VERSION     DAORNH010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16289_length_4105_cov_2.588148"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK264_23355"
     CDS             <1..812
                     /locus_tag="PK264_23355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRD78839.1"
                     /translation="LLTQRFGEAPPPLAPEPARDAAFARIAGRGVVRRFKPEPLPAAL
                     IDTLAALALSSPSKSDLQGRDILIVEDRAIRQRIDTLLADQDWVPGAPHLAIFLGNNR
                     RQRQVHEWRGHTFANDHLDAFFNAAVDAGIALSAFVLAAEAAGVGCCPLSVIRNHSPE
                     IAHLCALPDHVFPVAGLGFGWPERPNWVSARLPLSATVHRDRYRETSADTVDAYDRRR
                     HAIAPYRTIRRERDLGRPAFYGWSEDKARQYSTPERADFGAYVRKIGFKLD"
     gene            864..1454
                     /locus_tag="PK264_23360"
     CDS             864..1454
                     /locus_tag="PK264_23360"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRD78840.1"
                     /translation="MPMYHDGNRELQAHFGSTQLADRLVERLWRERFKDDDKAFIESV
                     GFFFLATADRDGRPDCSFKGGPPGFAKVLAPDLLAFPDYDGNGMFKSLGNIKANPRVG
                     LLFIELGEKPKRLRVNGRATVHLDDPLRGQFVGAQAVVRVIPEHIFPNCPRYIPDLGH
                     GTASEYLPAAGETPKEPAWKGFDAFKDVVPPRSDGN"
     gene            1533..2639
                     /gene="aroC"
                     /locus_tag="PK264_23365"
     CDS             1533..2639
                     /gene="aroC"
                     /locus_tag="PK264_23365"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRD78841.1"
                     /translation="MSANTFGHMFRFTTWGESHGAAIGCVVDGCPPGIPLVEADIQRY
                     LDRRRPGQSRFTTQRQEPDEVSILSGVFADDKGRQVTTGTPIALHIQNVDQRSKDYGE
                     IAEKFRPGHADYTYTAKYGIRDYRGGGRSSARETAMRVAAGAIARKVLAGFAIRGALV
                     QIGPHRIDRDRWDWAAVDRNPFFSPDATAAEQWADYLDGVRKAGSSVGAVIEVVAEGV
                     PAGLGAPLYGKLDADLASALMSINAVKGVEIGDGFAAANLTGESNADAMRAGNDGRPV
                     FLSNHAGGILGGISTGQPIVARFAVKPTSSILTPVPSIDRHGRDTEVRTKGRHDPCVG
                     IRAVPVGEAMMALVLADHLLRHRAQCGGPRPSEG"
     gene            2641..>4105
                     /locus_tag="PK264_23370"
     CDS             2641..>4105
                     /locus_tag="PK264_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRD78842.1"
                     /translation="MPLLRGPYFRSNLGTLVRGVGQLPVMISSWRGRRRASPAWTVAT
                     ALILAMVALPVVTIALIGLKGGDGTWSHLASTVLPASLRDTAILLVGVGTLTLIIGTG
                     TAWLVTMYRFPGRGLLDRLLVIPLAMPTCLVAYCYVDLLDFDGPPQALLRWLTGYTAR
                     RDYWFPDVRSMTGAILMLSSVLYPYVYLTARASFVQQSVCALEVARTLGRTPLGAFWS
                     VALPLARPALAGGVALVLMECLNDIGAVQHLGIPTLAAAIQDTWQQRSSLAGAAQIAI
                     VMLILVAILFAIESAVRSGGSVVQTTGRYRSLPFADLEGWRAVGATIACLLPFIAGFV
                     LPALVLLANAWRHWSPLYDSAFWIAAMHSFFLAGLAAMAAVLLAVVISYSRRVAANGF
                     TRPAARLAGLGYALPGTVLALGLLVALATLDTAIHAFAKSTLGITTGLILSGSVAALV
                     VAYAIRFLAVSLSAIDSGLARISPNLDAAARTLGETAL"
BASE COUNT          590 a         1492 c         1322 g          701 t
ORIGIN      
        1 agctgctgac gcaacgcttc ggcgaagcgc cgccgcccct cgcgcccgag cccgcccggg
       61 acgccgcctt cgcacgcatc gccgggcgcg gcgtcgtccg ccgcttcaag ccggagccgc
      121 tgccagcagc cctcatcgat acgcttgcgg cgctcgccct ctcctcgccc tcgaagagcg
      181 acctgcaggg ccgcgatatc ttgatcgtcg aggacagggc catccgccag cgcatcgaca
      241 cgctgctcgc cgaccaggac tgggtgccgg gcgcgccgca ccttgcgatt ttcctcggta
      301 acaaccggcg ccagcgccag gtccatgaat ggcgcgggca caccttcgcc aacgatcatc
      361 tcgacgcctt cttcaacgcc gcggtcgatg cgggcatcgc gctgtcggct ttcgtactcg
      421 ccgcggaagc cgccggcgtt ggttgttgcc cgctgagcgt gatccgcaac cattcgcccg
      481 agatagcgca cctttgcgcg ctcccggatc atgtattccc ggtcgctggc ctcggcttcg
      541 gctggccgga gcggccgaac tgggtgtcgg cgcgcctacc cctctcggcg accgtgcacc
      601 gcgatcgcta ccgtgagacg agcgccgata ctgtcgatgc ctacgacagg cgccgacatg
      661 cgatcgcgcc atatcgcacg atccggcgcg agcgcgacct cggcaggcct gccttctacg
      721 gctggtcgga ggacaaggcg cgccaatact cgacacccga gcgcgccgac ttcggcgcct
      781 acgtgcgcaa gattggtttc aagctcgact gacgaggcgg catgcgccgg atcagtcaag
      841 gggtaaaagc cagggaggtt gtcatgccga tgtaccacga cggcaatcgc gaactgcagg
      901 cacatttcgg ctcgactcaa ctagcggatc gactggtcga gcggctctgg cgcgagcgct
      961 tcaaggacga cgacaaggcc ttcatcgaat ccgtcggctt cttcttcctg gcgaccgcag
     1021 atcgcgacgg ccgcccggat tgctccttca agggcgggcc gcccggattc gccaaggttc
     1081 tcgcgccgga cctgctggcg tttccggact acgacggcaa cggcatgttc aagagcctag
     1141 gcaacatcaa ggcgaaccct cgggtagggc tgctgttcat cgagctcggc gagaagccga
     1201 agcgcctgcg cgtcaatggt cgcgcgaccg tccatctcga tgacccgctg cggggccagt
     1261 ttgtcggtgc ccaggcggtc gtgcgcgtca tcccggagca catcttcccg aactgtccgc
     1321 gctacattcc ggacctcggg catggcacgg catcggagta tctgccggca gcaggcgaga
     1381 cacccaagga gcccgcctgg aagggcttcg acgccttcaa ggacgtggtg ccgccgcgca
     1441 gcgacggcaa ttgacgacgc ggcgccgtca ccattgctgc ggcgcggcta cctctgtacg
     1501 ttccgcgcca cacgtcgcga aggcaaccaa ccatgtcggc caatacgttc gggcacatgt
     1561 tccgcttcac gacctggggc gagagccacg gggcggcgat cggctgcgtg gtggacggct
     1621 gtccacccgg catcccgctc gtcgaggctg acatccagcg ctatctcgat cgtcgcaggc
     1681 ccggccagtc gcgcttcacg acccagcgcc aggagccgga cgaggtgagt atcctctccg
     1741 gcgtgtttgc cgacgacaag ggccgccagg tcacaaccgg cacgccgatt gcgctgcaca
     1801 tccagaatgt cgaccaacgc tcgaaggact acggtgagat cgccgagaag ttccggcccg
     1861 ggcacgccga ctacacgtac acggcgaagt atggcatccg cgactatcgc ggcggcggcc
     1921 gctcctcggc gcgcgagacg gcgatgcgcg tcgcggccgg tgcaattgcg cgcaaggtgc
     1981 tcgccggctt tgccatccgt ggcgccctcg tccagatcgg cccgcacagg atcgatcgcg
     2041 atcgctggga ctgggccgcg gtcgaccgga acccgttctt ctcgcccgac gccacggccg
     2101 ccgagcagtg ggcggactac cttgacggcg tgcgcaaggc cggctcgtcc gtgggcgccg
     2161 tgatcgaggt ggtcgccgaa ggtgtgccgg cagggctcgg cgcgccgctc tacgggaagc
     2221 tcgacgccga cctcgcctcc gcgctcatgt cgatcaatgc ggtgaaaggc gtcgagatcg
     2281 gcgacggctt cgccgcggcg aacctcaccg gcgagagtaa tgccgatgcc atgcgcgcag
     2341 gcaacgacgg ccggcccgtg ttcctctcca accatgccgg cggcatcctc ggcggcatct
     2401 cgaccggtca gccgatcgtc gcccgcttcg ccgtgaagcc gacctcgtcc atcctgacac
     2461 ccgtgccgag catcgaccgt cacggccgcg acaccgaggt gcgcaccaag ggccggcacg
     2521 acccctgcgt cggcatccgc gccgtgcccg tcggcgaggc catgatggcg ctcgttcttg
     2581 ccgaccacct cctccgccat cgcgcccagt gcggcgggcc gaggccgagc gagggctgac
     2641 gtgccactgc ttcgcggccc ttattttcgt tcgaacctcg gcacgctcgt gcgcggcgtc
     2701 ggccagctcc ctgtgatgat cagcagctgg cgcgggcggc ggcgcgcctc gccagcctgg
     2761 acggtcgcaa ccgccctgat cctcgccatg gtggcactgc cggtggtgac gatcgcactc
     2821 atcggcctca agggcggcga cggcacctgg agccacctcg cctcgacggt gctacccgcc
     2881 tcgctccgcg ataccgcaat cctgctcgtg ggcgtcggga cgctcacgct gatcatcggc
     2941 acggggacgg cctggctcgt caccatgtac cgctttcccg gtcgcggcct cctcgaccgg
     3001 ctcctcgtca ttccgctggc gatgccgacg tgcctcgttg cctactgtta cgtcgacctc
     3061 ctcgatttcg acggcccgcc acaggcgttg ctgcgctggc tgacaggcta caccgcgcgg
     3121 cgcgactact ggtttccgga cgtgcgctcg atgacggggg cgatcctgat gctgtcgtcc
     3181 gtgctctacc cttacgtcta cctcaccgcg cgcgcgagct tcgtccagca gtcggtctgc
     3241 gcactcgaag tggcgcgcac actcgggcgg acgccgctcg gcgccttctg gtctgtggca
     3301 ctgccgctcg cccggccggc gctcgcaggt ggtgtggcgc tcgtgctgat ggaatgcctc
     3361 aacgacatcg gtgcggtgca gcatctcggc atcccgacgc tcgcggccgc catccaggac
     3421 acctggcagc agaggagctc gctcgccggt gcggcccaga tcgccatcgt catgctgatc
     3481 ctggtcgcca tcctgttcgc catcgagagc gcggtgcgca gcggcggctc cgtcgtccag
     3541 acgacggggc ggtaccgctc actgccgttc gccgatctcg agggctggcg cgcggtcggg
     3601 gcaacaatcg cctgcctcct ccctttcatt gcggggttcg tgttgcccgc gctggtgctg
     3661 ctcgccaacg cgtggcgcca ctggagcccc ctctacgaca gtgccttctg gattgcggca
     3721 atgcacagtt tcttcctggc cgggctcgct gccatggcgg cggtgctgct cgccgtcgtg
     3781 atctcctatt cgcgccgcgt cgccgccaac gggttcacgc ggccggcggc gcgtctcgca
     3841 gggctcggct acgcgttgcc cggcaccgtg ctcgccttgg gtctcctcgt cgcgctcgcg
     3901 accctcgaca ctgcgatcca cgcattcgcg aagagcacgc tcggcatcac caccgggctc
     3961 atcctgtcgg gttccgtcgc ggcgctcgtc gtcgcctatg cgatccgctt cctcgccgtg
     4021 tcgctcagtg ccatcgattc gggcctcgcg cgcatctccc cgaacctcga cgccgctgcg
     4081 cggaccttgg gcgagacggc gcttt
//
LOCUS       DAORNH010000364         4100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_16318_length_4100_cov_3.069468, whole genome shotgun sequence.
ACCESSION   DAORNH010000364 DAORNH010000000
VERSION     DAORNH010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16318_length_4100_cov_3.069468"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PK264_23375"
     CDS             complement(<1..1770)
                     /locus_tag="PK264_23375"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD78843.1"
                     /translation="MLKRLLAAAAALVGVGLSQPALAGPLDYAVIITNKGYASYKHTH
                     DVKYAHEDGKAFKRALIEVFKVPETRIIEIQDASLGRLHEHFGNPKAREGTIYDFLKH
                     PKGRLFVYYSGHGVPGFTRSDPNTPKPYLLPVDSNPQLVASSGYSLDDLVNRLREIKT
                     ERLFEGEVLLFLEACFSGVSPAGAILDKSSGTAFNPVLPASSGASSDIFILAASRSDE
                     IASWDDKAKLGVFTNALLDGIYGLADQDMWKGNGDRKLTLGELRRFTESRMSDRLRQL
                     YKGATRRQSPTFTGTDTATLVEYAASTYPVRDARVMTEEETQCRILKSSTDRTRISKF
                     LEDCDYCLCRADLEKQVAKLDVDLQFCDSEREAAGRLYGEGNLDELGTLISGARCAAI
                     KTEFVAKVDELKRTCADERKLFEDMRQKRALDRMRTWVARSSSCASVRNEARRYVDGQ
                     TQACERAGKQFAEVKAAGDPETLRRFARFADCESIKVDANREADDLDRICVTDLTLLK
                     RHMLDKRDHMVTNMSNMSKCGEVRERAKKFLEALEARCGTDATAWEPLKTSKDLVALR
                     KVLANAECPRVRTEIERRIGVVEE"
     gene            complement(1837..3288)
                     /locus_tag="PK264_23380"
     CDS             complement(1837..3288)
                     /locus_tag="PK264_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78844.1"
                     /translation="MLPRFATVLLALLPWLLPSPAAADQPMRKPVAALVDYAQVCHAK
                     RFAQPAVEMTPYGPRLASGAAAAPLRVVYTGFPVRTSLSPDLQEHINRIILRRLSEAR
                     MDIMDPREQAEALEAVRGSFGTNKMLEASKGMKLQSGDMLVRAEGRFIRNGQYVEVGL
                     VLMWPDTSCKNETPTDNVEVVDDGAAVPIDVMREAIKSFTTNYKVINRIAVLPTVIGV
                     SPLDPAIAGPLEDDLERELAKALKPAAQPGQTVIAGADDKRSAPPPVPLARMSRLSFF
                     KEEAKEGDWLARLRVEVRASGPHLTVEFLDVMGSGSASAGRMIAPASIRSAKPAAPAI
                     ELLAEQSPLRVDLDPLRYAFKVNRPASLYCAMQDLDGETLLIYPTPDTQRNKHQPRAE
                     AMRAPQDLDGTIAGTFGKPVRALIRCLAVPNGLTGNLERAWMLGTVRERLENKKPVKL
                     SGAEYAKLVDALKAGAEVHETHAILTVTGTAPR"
     gene            complement(3315..>4100)
                     /locus_tag="PK264_23385"
     CDS             complement(3315..>4100)
                     /locus_tag="PK264_23385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78845.1"
                     /translation="KPKPGAEDPKSLAGTPGKAATQPGPASPEPGASDPKPADPKRTD
                     VAGPNPGQTPPKTVEPAPIDRGKAEPSDKAPVPNPAVVVTPGKADNEDTKTGKAADPA
                     SGDSTATKPGPPAKTATDPTPVPQPAAAAGDDLVVIEELRAPAGSNCIQIISGERLPE
                     IFVNKPKDGRFHTSDANGLCAIVYRRLEGAAAMTLSVDETLIRATADFATFGRGKVRL
                     NAGDVARRVRDDFRTQMTYSLWLKSSASPGSANERRYVHELRR"
BASE COUNT          648 a         1289 c         1396 g          767 t
ORIGIN      
        1 ttcctcgacg acgccgatac ggcgctcgat ctcggtccgg acgcgcgggc actcggcgtt
       61 ggcgagcacc ttgcgaagtg cgaccaggtc cttgctcgtc ttcagcggtt cccaggccgt
      121 ggcgtccgtg ccgcaccgtg cctccagcgc ctcgaggaac ttcttggcgc gctcgcgcac
      181 ctctccgcac ttcgacatat tcgacatgtt cgtgaccatg tgatcgcgct tgtcgagcat
      241 atgacgcttg agcaacgtca ggtcggtgac acagatccga tcgagatcgt cggcctcgcg
      301 gttcgcgtcg accttgatcg actcgcagtc ggcgaagcga gcgaacctgc gcagcgtctc
      361 cggatcgcct gcggccttca cctcggcgaa ctgcttgcct gcccgctcgc acgcctgggt
      421 ctggccatcc acatagcgac gcgcctcgtt gcgcacgctg gcgcagctgg acgacctcgc
      481 cacccacgtc cgcatgcgat cgagcgcgcg cttctggcgc atgtcctcga acagcttgcg
      541 ctcgtcggcg cacgtgcgct tgagctcgtc gaccttcgcc acgaattcgg tcttgatcgc
      601 cgcgcagcgc gcgccggaga tcagcgtgcc gagctcgtcg aggttaccct cgccgtagag
      661 gcggccggcc gcctcacgct cgctgtcgca gaactgaaga tcgacgtcta gcttggccac
      721 ctgcttctcg aggtcggcgc ggcagaggca atagtcgcaa tcctcgagga acttcgagat
      781 gcgcgtgcgg tcagtgctcg atttcaggat gcggcactgc gtctcttcct cggtcatgac
      841 tctcgcgtca cgcacgggat aggtggatgc agcatactcg acgagcgtcg cagtgtcagt
      901 acctgtaaag gtcggcgact gccggcgcgt tgcgcccttg tagagctggc gcagacggtc
      961 gctcatgcgg ctctcggtga agcggcggag ctcaccgagg gtgagcttgc ggtcgccgtt
     1021 gcccttccac atgtcctgat cggcgaggcc gtagatgccg tcgagcaggg cgttcgtgaa
     1081 cacgccaagc ttcgccttgt cgtcccagct cgcgatctcg tcggagcggc tggccgcaag
     1141 aatgaagatg tcgctgcttg cgccgctgga cgcaggcagt acgggattga atgccgtgcc
     1201 ggacgatttg tcgaggatcg cgcccgcagg actcacgccg gagaagcacg cctcgaggaa
     1261 gaggagcacc tcaccctcga acaggcgttc ggtcttgatc tcgcgaagcc gattgacgag
     1321 atcgtcgagc gaatagccgg acgaggcaac gagctgcggg ttcgaatcga ccggcagcag
     1381 atagggcttc ggcgtattgg gatcggaacg cgtgaatccg ggcacgccgt gtccggagta
     1441 atagacgaag aggcgccctt tcgggtgctt caagaaatca tagatggtcc cctcgcgggc
     1501 cttcgggttg ccgaagtgct catggaggcg gccgaggctc gcgtcctgga tctcgatgat
     1561 acgcgtctcg ggaaccttga agacctcgat cagggcgcgc ttgaacgcct tgccgtcctc
     1621 gtgggcgtac ttcacgtcgt gcgtgtgctt gtaggacgca tagcccttgt tggtgatgat
     1681 caccgcatag tcgagcgggc cggcgagggc cggctgcgag agccctacac cgaccagagc
     1741 cgcagcagca gcgagcaatc tcttgagcat ggcgagctac ctcctcaagc gcattgcgac
     1801 atccaaacgc gatttcgtca cttgagcctc ggacggttaa cgtggcgcgg tcccggtgac
     1861 agtcaggatg gcatgagtct catgaacctc ggcgccggcc ttgagcgcgt cgacgagctt
     1921 tgcatattcc gcgccggaga gcttgaccgg tttcttgttc tcgaggcgct cacgcaccgt
     1981 gcccagcatc caggcgcgtt ccaggttgcc ggtgagcccg ttcggaaccg ccagacagcg
     2041 gatgagcgcg cgaacgggct tgccgaaggt gcccgcgatg gtaccgtcga ggtcctgtgg
     2101 cgcccgcatt gcctcggcac gcggctggtg cttgttccgc tgggtatcgg gcgtcgggta
     2161 gatgagcagc gtctcaccgt cgagatcctg cattgcgcag tagagcgagg ccggccggtt
     2221 gaccttgaac gcgtaacgaa gaggatcgag gtccacgcgg agcggcgact gctcggccaa
     2281 gagctcgatt gccggcgcgg cgggtttggc cgagcggatc gacgccggcg caatcatccg
     2341 cccggccgag gcgctgccgg agcccatcac gtcgaggaac tccacggtga ggtgcgggcc
     2401 gcttgccctc acctcgacgc gcaggcgcgc gagccagtcc ccctccttcg cctcctcctt
     2461 gaagaaggag aggcgcgaca tgcgcgccag cggtacgggt ggcggtgccg agcgcttgtc
     2521 gtcggcgccg gcgatcactg tctggcccgg ttgggctgcg ggtttcaatg ctttggcgag
     2581 ctcgcgctcg agatcgtcct cgagggggcc ggcaatcgcc ggatcgagcg ggctgacgcc
     2641 gatcaccgtc ggcagcacgg cgatgcggtt gatcaccttg tagttggtcg tgaacgattt
     2701 gatggcctcg cgcatcacgt cgatcggcac cgcagcgccg tcgtcgacca cctcgacatt
     2761 gtcggtcggc gtctcgttct tgcagctcgt gtcgggccac atcaggacga ggccgacctc
     2821 gacatactgg ccgttgcgaa tgaaacgccc ctcggcgcgc acgagcatgt cgcccgactg
     2881 cagcttcatg cccttggagg cttcgagcat cttgttggtg ccgaatgagc cgcgcacggc
     2941 ctcgagcgcc tcggcctgct cgcgcgggtc catgatgtcc atgcgcgcct cgctcaggcg
     3001 gcgcaggatg atgcggttga tgtgctcctg gaggtcgggc gagaggctcg tgcgcacggg
     3061 aaagcccgtg tagacgacgc gcaatggcgc ggcggcggcg ccagaagcga gccgcgggcc
     3121 ataaggtgtc atctcgaccg cgggctgggc gaagcgctta gcgtgacaga cctgcgcgta
     3181 gtcgaccagc gcggccacag gcttgcgcat gggctgatcg gccgccgcag gcgacggcag
     3241 cagccaaggc agcagcgcga gaagcacggt ggcgaaacgg ggcagcatgt cgtccctctt
     3301 cgggattaaa ggtactaacg gcgcaattcg tgaacgtaac gtctttcgtt ggcagatcca
     3361 ggcgaagcgc ttgatttcaa ccacagagaa taagtcatct gcgtgcgaaa gtcatctctc
     3421 acgcggcgcg cgacgtcgcc cgcgttgagg cgaaccttgc cacgaccgaa ggttgcaaaa
     3481 tcggccgtgg cgcgaatgag ggtctcgtcg acgctgagcg tcattgcagc ggcaccttcg
     3541 agccggcggt agacgatggc gcagaggccg ttggcgtcgc tcgtatggaa gcggccgtcc
     3601 ttgggcttgt tgacgaaaat ttcaggcagc cgctcgccgg agatgatctg aatgcaattc
     3661 gagccggccg gtgccctgag ctcctcgatg accacgaggt cgtcgccggc agctgcggcg
     3721 ggctgaggca caggcgtcgg gtcggttgca gtcttcgcgg gggggccagg cttcgtcgct
     3781 gtgctgtcgc ccgacgccgg atccgcagcc ttcccggttt tcgtatcctc attgtccgcc
     3841 ttgccgggtg tcacgacgac tgccggattg ggcacgggcg ccttgtcgct cggctccgcc
     3901 ttgccgcgat cgatcggtgc cggctcgacg gtcttgggag gcgtctggcc gggattgggg
     3961 ccggcgacgt cggttcgctt ggggtcagcc ggcttcggat cggaggcccc gggctcggga
     4021 gaggccggcc ctggctgcgt cgcggccttg ccaggtgtgc ctgctagcga cttcgggtcc
     4081 tcggcgcctg gtttcggctt
//
LOCUS       DAORNH010000365         4033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_16680_length_4033_cov_2.480895, whole genome shotgun sequence.
ACCESSION   DAORNH010000365 DAORNH010000000
VERSION     DAORNH010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16680_length_4033_cov_2.480895"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>312)
                     /locus_tag="PK264_23390"
     CDS             complement(<1..>312)
                     /locus_tag="PK264_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD78846.1"
                     /translation="EEGCEFFKDVPAIRDKLETLARVGLGYIKIGQQATTLSGGEAQR
                     IKLAKELSRRATGRTLYILDEPTTGLHFHDVAKLLEVLHELVEAGNTVAVIEHNLEVI
                     KT"
     assembly_gap    313..491
                     /estimated_length=179
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<492..>702)
                     /locus_tag="PK264_23395"
     CDS             complement(<492..>702)
                     /locus_tag="PK264_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78847.1"
                     /translation="LREQAGLHDKLEGLEHLDKVIDIDQSPIGRTPRSNPATYTGAFT
                     PIREWYTGLPEAKTRGYGPGRFSFNV"
     assembly_gap    703..721
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<722..2879)
                     /gene="uvrA"
                     /locus_tag="PK264_23400"
     CDS             complement(<722..2879)
                     /gene="uvrA"
                     /locus_tag="PK264_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRD78848.1"
                     /translation="MRKPAKSGNGRTPAAAAGSNIVAARTATAAADPHRTIHVRGARE
                     HNLKNVDLVIPRDALIVFTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLEMMQ
                     KPDVDHIDGLSPAISIEQKTTSRNPRSTVGTVTEIYDYLRLLFARVGVPYSPTTGLPI
                     ESQTVTQMVDRVLALPEGTRLMLLAPVVRGRKGEYRKELAEWQKKGFQRVKINGTVHE
                     VSEAPKLDKKYKHDIDVVIDRLVVRPEIATRLADSFETALGLADGLAIVELVDKPLAA
                     SPSPRSSRGDGEGAGTEKEVLRNKNETHERIIFSQRFACPVSGFTIEEIEPRLFSFNA
                     PAGACPKCDGLGTELKFEPDLVVPDVELSLADGAVVPWARTGNTSPYYEQTLEALALH
                     FGVSMQTPWEKLPANVREAILFGTKGEEITFVYEDGLRRYQTQKTFEGVIGNIERRWR
                     ETDSSWVREELARYQNATPCEACKGYRLKPQSLAVKVAGKHIGEVGDLSIRAANDWFD
                     ALPATLTAKQREIAVRILKEISERLKFLVDVGLDYLTLSRNSGTLSGGESQRIRLASQ
                     IGSGLTGVLYVLDEPSIGLHQRDNARLLETLRHLRDLGNTVIVVEHDEDAVLTADHVV
                     DIGPGAGVHGGRIIAQGTPADIMASPESLTGQYLSGAKEVPVPARRRKRGNRELKVMG
                     ARSNNLKSVTAAIPLGLLTCVTGVSGGGKSTFLIDTV"
     gene            3035..>3380
                     /locus_tag="PK264_23405"
     CDS             3035..>3380
                     /locus_tag="PK264_23405"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRD78849.1"
                     /translation="MNKTILAELAPTGVLRAGVNLSNFLLVTGKTASGDPIGVSPDMA
                     KAIADKLGVPVRYVTFKSPGELADQAGKNVWDIGLIGAEPQRAETIAFTAAYTEIQST
                     YLVPAGSAIRTIA"
     assembly_gap    3381..3416
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3417..3738
                     /locus_tag="PK264_23410"
     CDS             <3417..3738
                     /locus_tag="PK264_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78850.1"
                     /translation="RDRNIKHATLVKSDTLDSSFEDFVSKKLEALAGLKPRLLTDIEK
                     VPGARILDGQFSAVQQAIGTAKANSVGARFLSEFVEEAKRSGLVAGFIAKHNVKGLSV
                     APPA"
     gene            complement(3741..>4033)
                     /locus_tag="PK264_23415"
     CDS             complement(3741..>4033)
                     /locus_tag="PK264_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HRD78851.1"
                     /translation="WQPHPGNPLVTDARRARPAGELFREGGRLYRPVQDCSGGYGSAL
                     AINEIERLDTTGFRERQVLHTRFAPSERTLGPHTLNVVGDFELIDLFGPRQK"
BASE COUNT          595 a         1225 c         1305 g          674 t
ORIGIN      
        1 ggtcttgatt acctcgagat tgtgctcgat cacggcgacg gtgttgcccg cctcgaccaa
       61 ctcgtgcagc acttcgagca gcttcgcgac gtcgtggaaa tgaagccccg tcgtcggctc
      121 gtcgaggatg tagagcgtgc gtcccgtggc gcgccgcgag agttccttgg cgagcttgat
      181 gcgctgcgcc tcgccccccg agagtgtcgt cgcctgctgg ccgatcttga tgtagccgag
      241 gccgacgcgg gcgagcgtct cgagcttgtc gcggatcgcg ggaacgtcct tgaagaactc
      301 gcagccctcc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nacgttgaac gagaagcggc cggggccata gccgcgcgtt ttcgcctcgg
      541 ggagccccgt gtaccactcg cggatcggcg tgaaggcgcc cgtgtacgtc gccggattgg
      601 agcgcggtgt gcgcccgatc ggcgactggt cgatgtcgat caccttgtcg aggtgctcga
      661 gcccttcgag cttgtcgtgg aggcccgcct gctcgcggag ccnnnnnnnn nnnnnnnnnn
      721 nagaccgtgt cgatgaggaa cgtggacttg ccgccgccgg agacgccggt gacgcaggtg
      781 aggaggccga gcgggattgc cgccgtcacg ctcttcaggt tgttggatcg cgcgcccatg
      841 accttgagct cgcgattgcc ccgtttgcgc cgtcgcgccg gcaccggcac ttccttcgcg
      901 cccgagagat actggcccgt gaggctctca ggcgaggcca tgatgtcggc gggcgttccc
      961 tgcgcgatga tgcgcccgcc atgcacgccc gcgccggggc cgatgtcgac cacgtggtcg
     1021 gccgtcagca ccgcgtcctc atcatgctcg accacgatca ccgtgttgcc gaggtcgcgc
     1081 agatgccgca gcgtctcgag aaggcgggca ttgtcgcgct gatggaggcc gatggaaggc
     1141 tcgtcgagca cgtagaggac gccggtcagg cccgagccga tctggctggc gaggcggatg
     1201 cgctggctct cgccgcccga tagcgtgccg gagttgcgcg aaagcgtcag gtagtcgagg
     1261 cccacgtcaa caaggaattt gagccgctcg ctgatctcct tcaggatgcg gaccgcgatt
     1321 tcgcgctgct tggccgtgag cgtggcgggg agcgcatcga accagtcgtt ggcggcgcgg
     1381 atcgagagat cgccgacctc gccgatgtgc ttgccggcga ccttcacggc gagcgactgc
     1441 ggcttcaggc gatagccctt gcaggcctcg cacggtgtcg cgttctggta gcgggcaagc
     1501 tcctcgcgca cccaggagct gtcggtctcg cgccagcgcc gctcgatgtt gccgatgacg
     1561 ccctcgaacg tcttctgcgt ctggtagcgg cggagcccgt cttcgtagac gaaggtaatc
     1621 tcctcgccct tggtgccgaa gagaatggcc tcgcgcacgt tggcagggag cttctcccac
     1681 ggcgtctgca tgctgacgcc gaagtggagc gcgagcgcct ccagcgtctg ctcgtagtag
     1741 ggcgacgtat tgccggtacg cgcccagggt acaaccgcgc cgtcggcgag cgacagctcg
     1801 acatccggca cgacgagatc gggctcgaac ttgagctcgg tgccaaggcc gtcgcacttc
     1861 gggcaggcgc cggccggcgc attgaacgag aagagccgcg gctcgatctc ctcgatggtg
     1921 aagccggaga ccgggcaggc gaagcgctgc gagaagatga tgcgctcgtg cgtctcgttc
     1981 ttgttgcgca gcacctcctt ttcagtgccg gcgccctcgc cgtctccacg cgacgaacga
     2041 ggagaggggg aagcggcgag aggcttgtcg acgagttcca cgatggcgag gccgtcggcg
     2101 aggccgagcg cggtctcgaa gctgtcggca aggcgggtcg cgatctcggg ccggacgacg
     2161 aggcgatcga tcacgacgtc gatgtcgtgc ttgtacttct tgtcgagctt cggcgcctcc
     2221 gacacctcat gcacggtgcc gttgatcttg acgcgctgga agcccttctt ttgccactcg
     2281 gcgagctcct tgcggtactc gcccttgcgg ccgcgcacga ccggcgcgag cagcatcagg
     2341 cgcgtgcctt cgggcagcgc cagcacgcgg tcgaccatct gcgtgacggt ctggctctcg
     2401 atcggcaggc cggttgtcgg cgagtagggg acgccgacgc gcgcgaacag gaggcggagg
     2461 tagtcgtaga tctcggtcac cgtgccgacg gtcgagcgcg ggttgcggct cgtcgtcttc
     2521 tgctcgatcg agatcgcggg ggagagcccg tcgatatggt cgacatcggg cttctgcatc
     2581 atctcgagga actggcgggc gtacgccgag aggctctcga cgtagcggcg ctggccttcg
     2641 gcatagatcg tgtcgaaggc cagcgaggat ttgcccgagc ctgagagccc ggtgaacacg
     2701 atcagggcat cgcgcggaat gacgagatcg acgttcttca ggttgtgctc gcgggcgccg
     2761 cgcacatgga tggtccgatg cgggtccgcc gcggccgtgg ccgtgcgtgc cgcgacgata
     2821 ttcgatccgg ccgctgccgc aggcgttcgg ccgttacccg actttgctgg ctttctcatg
     2881 cctcgtcact accggatcgc agttggcccg gcaatcgagc gcgaggtagc ctctgtgcaa
     2941 ggtcgggatg cgcatttctc ctgctgacgc ccgccatgcg gggctgtaag cctgagcgat
     3001 cgtcacgaca tgtcccggag cggaggctga aaccatgaac aagacaattc tggccgagct
     3061 ggcgccgacc ggcgtcctcc gcgccggcgt caacctctcg aacttcctgc tcgttaccgg
     3121 taagacggca agcggcgatc ccatcggcgt ctcccccgac atggcgaagg cgattgccga
     3181 caagctcggc gtgccggtcc gctatgtgac cttcaagtct ccgggagagc tcgccgacca
     3241 ggccggcaag aacgtgtggg acatcggcct catcggcgcc gagccgcagc gcgccgagac
     3301 gatcgcattt accgccgcct acaccgagat ccaatctacc tacctcgtgc cggcgggctc
     3361 ggccatccgg acgattgccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcgc
     3421 gaccgcaaca tcaagcacgc cacactcgtg aagtcggaca cgctcgacag ctcattcgag
     3481 gatttcgtgt cgaagaagct cgaggccctc gcggggctca agccgcgtct tctcaccgac
     3541 atcgaaaagg tgccgggcgc ccgaatcctc gatggccagt tcagcgccgt ccagcaggcg
     3601 atcggcacgg cgaaggccaa ctcggtcggt gccaggttcc tctccgagtt cgtcgaggaa
     3661 gcgaagcgca gcggactggt cgcaggcttc atcgccaagc acaacgtgaa ggggctctcg
     3721 gtggcgccac cggcctgacg tcacttctgg cgcgggccga agaggtcaat cagctcgaaa
     3781 tcgcccacca cgttcagtgt gtgcggtccg agcgtgcgct cggacggcgc gaagcgcgtg
     3841 tggagtacct gtcgttcgcg aaatccggtt gtgtcgagcc gttcgatctc gttgatcgcg
     3901 agcgcgctgc catagccgcc gctgcagtcc tggacgggac ggtagagcct gcctccctcg
     3961 cggaagagct cgcccgccgg tcgcgcgcgt cgcgcatcgg tgacgagcgg atttcccggg
     4021 tgcggctgcc acg
//
LOCUS       DAORNH010000366         3944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_17176_length_3944_cov_2.799949, whole genome shotgun sequence.
ACCESSION   DAORNH010000366 DAORNH010000000
VERSION     DAORNH010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17176_length_3944_cov_2.799949"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            365..958
                     /locus_tag="PK264_23420"
     CDS             365..958
                     /locus_tag="PK264_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRD78852.1"
                     /translation="MSTRLIELATMGELAYRFLIDVVAMLALVFAIYYPRYRDKELVT
                     AASLFNVFAFSVLSVLSSVQFSMAAGFGLFAILALFTLRSEPISRIDITYFFGAVAIA
                     VICAIPGTSLGFVALVAGIVLIGAWLLDHPAILASADGVKITLDKIDNHALSDPAAMR
                     SELSKRLGVEVTSYQITALDYINDMARLNVYFRKPRI"
     gene            970..1752
                     /locus_tag="PK264_23425"
     CDS             970..1752
                     /locus_tag="PK264_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRD78853.1"
                     /translation="MESLIDTSLSRFSPISLDRLNQKAAMLERLDNKYIVQAARLVAP
                     LEEFAAHFDILEIDGRRSFTYATRYYDDWDLRSYFDHHQGRRQRSKIRIREYVDAGFS
                     YLEVKLKDKRDITIKKRLKLQNPKMDLGDRELKFIDECHQEIYATPFDKKLKPVIDMQ
                     YRRITLVAREGGERMTLDTCIEFRASNGGTAVDPGAFILETKSSNGNGLADRVLRSHH
                     LHPTKRCSKYCIGMAATGQVQKRNRFLPGLRKLALVGALRAV"
     gene            1799..2764
                     /locus_tag="PK264_23430"
     CDS             1799..2764
                     /locus_tag="PK264_23430"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HRD78854.1"
                     /translation="MNSLGRLAIAVAPLGLLLALFWIGTARAQQADRPLTATTLDVVT
                     LDPERSPLSELREISGLAWDGDTKRLHAVSDGRRLLQLSLSGLPDKIGTMTVLARSHL
                     TLAGGARSRKGDLNPEGLALVPVAERVTAAAELLVVSEAPPRLVVVDLNGAMLREVEL
                     PAALASIANLSAPKNGLESVTILPGMGYLTAPERPLRDHDRSRHVIFSSHGPRLHYTT
                     EALGKTSIKAMDSLGTDRIVILERTRSNEGLIVPYLRVIEPRRCTQEAPCPGRVAKVT
                     VPGITDADFEAIAHLGGDRFLIASDDRIAGRFRTVFALIAVAGLD"
     gene            2859..3656
                     /locus_tag="PK264_23435"
     CDS             2859..3656
                     /locus_tag="PK264_23435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD78855.1"
                     /translation="MIQHTRTGKGRPPIVLVHGFGCARSDWDLQVAHLAQHSETIAVD
                     LGGHGTTPGTSAHARLETHGADVAELLRGLDLRGAVLIGHSMGCRVIMEAAAREPARV
                     AGVILVDGSRMGQAGVNAHTPLLAEIKAKGGYASYIRPVFESMFAKTYPADKAAPIVE
                     RAARMPAEIAGPLFADIRRYDSEHMDRILSLQRWPFLAIQTTRIDQGGNRVSMKSGET
                     TPYLDLIRSKVAGARIEIIPSIGHFPQVEAPAALNQLLERFVVSLRS"
     gene            3711..>3944
                     /locus_tag="PK264_23440"
     CDS             3711..>3944
                     /locus_tag="PK264_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78856.1"
                     /translation="MQSAGITSLLARGRVMPEACCACRQPRTTRNQQRSIISWLRTRR
                     SHAPSDNPEQILGARPFRESGKDRLEALVDQQHR"
BASE COUNT          746 a         1301 c         1191 g          706 t
ORIGIN      
        1 ggtcgcgcag caccgatgcg tcagagccgg acggtcgacg ctttccttgc acggagatga
       61 cgtgaccggt cagcttggcg tccatcgccg acggtcaatc tcgtcacgcc aggatattcc
      121 tccaacccgc accgccctcg agcgccatgc gacgccgcga agcaactcga gcgtccatgg
      181 cgaccgaaag aaagaacagg ctacggcgga attgcggatt tccggcggac tctgcaccgc
      241 aattcgcgga atagcctgtc accctacgcg ttctcgccat gtgacagtcg aatgacggtt
      301 tgatgacaac ggcgccggag gccgcagcat caacacacag cagtcttgaa caggagatcc
      361 tattgtgagc acgcggctta tcgaactggc gacgatgggc gaattggcct accggtttct
      421 gatcgacgtc gtcgcgatgc ttgccctggt ttttgcaata tactacccgc gatatcgcga
      481 caaagagctc gtgacggccg catcgctgtt caatgtcttt gcattcagcg tcctctcggt
      541 tctctcgtcc gtccaattct cgatggccgc gggattcggc ctgtttgcca tcctggcctt
      601 gttcacgctc aggtccgagc cgatttcaag gatcgacatc acttacttct tcggggctgt
      661 cgcgatcgcc gtgatctgcg cgatcccagg aacgtcgctc gggttcgtcg cgctcgtcgc
      721 cggcatcgtg ctgatcggcg cctggctgct cgatcatccg gcgatccttg cctccgccga
      781 cggcgtgaag atcaccctcg acaagatcga caatcacgcc ctgtcggacc cggccgccat
      841 gcgctccgag ctctcgaagc gcctcggcgt cgaggtgacg tcctaccaga tcacagcgct
      901 cgactacatt aacgacatgg cacgcctcaa cgtctacttc cgcaaaccgc gaatctgagg
      961 ggaatggaca tggaatcgct catcgacacg tcattgtcgc gcttctcgcc gatctcgctc
     1021 gatcgcctca accaaaaagc ggccatgcta gagcggctcg acaacaagta tatcgtgcag
     1081 gccgcccggc ttgtcgcgcc gctcgaggaa tttgccgcgc atttcgatat tctcgagatc
     1141 gacgggcgcc gctcgttcac ctatgcgacg cgctactacg atgattggga cctgcggagc
     1201 tatttcgacc atcatcaggg ccggaggcag cgctccaaga tccggattcg tgaatacgtg
     1261 gatgcgggat tcagttacct cgaggtcaag ttgaaggaca agcgcgacat caccatcaag
     1321 aagcggctca agctccaaaa tccgaaaatg gacctagggg accgcgaact gaaattcatc
     1381 gacgagtgcc atcaggagat ctacgcgacg cccttcgaca agaagctgaa gccggtaatc
     1441 gacatgcagt atcggcgcat aacgctcgtc gcgcgcgagg gtggggagcg catgacgctg
     1501 gacacgtgca tcgaattcag agcgagcaat ggcgggaccg cggtcgatcc tggagctttc
     1561 atcctcgaga cgaaatccag caacggcaat ggcctggcgg atcgcgtgct gcgctcgcat
     1621 cacctgcatc cgacgaagcg ctgctcgaaa tattgcatcg gcatggccgc aaccggccag
     1681 gtccagaagc gtaaccgttt tcttcccggg ctccgaaagc tcgcccttgt gggtgcgctt
     1741 cgggcagtgt gaggacatga tggcagcacc ccgaagtgat cttgggagca cgaagcccat
     1801 gaatagtctc ggccgccttg cgattgccgt cgctcccctg gggctgctgc tggcgctctt
     1861 ctggatcgga accgcgcggg cgcagcaggc ggatcgcccg ctcactgcga ccacgctcga
     1921 cgtcgtgaca ctcgatccgg agcgcagccc gctgagcgag ttgcgcgaaa tttctggcct
     1981 cgcctgggac ggcgacacga aacggctgca tgccgtctcc gacggacgac gccttcttca
     2041 actctcgctc tcgggcctgc ccgacaagat cggcacgatg acagtgctgg cacggtccca
     2101 cttgacactc gcgggtgggg cgaggagccg caaaggcgac ttgaaccctg aaggtctggc
     2161 gcttgtcccg gtcgcagagc gtgtcaccgc ggcggccgag cttctggtcg tcagcgaggc
     2221 gccgccgcgc ctcgtcgtcg tcgatctgaa cggcgccatg cttcgggaag tggaactgcc
     2281 agctgcgctc gccagcatcg caaatctgag tgctccgaag aacggtctcg agtcggtgac
     2341 gatacttccc gggatgggct acctgaccgc accggaacgg ccactccgcg atcatgatcg
     2401 aagccgtcac gtgatcttct cgagccacgg tccgcgcctc cactacacca cggaggcgct
     2461 cggcaaaacg agcatcaagg cgatggactc gctcggtacc gacaggatcg tcattctgga
     2521 gcgcacgcgg tccaatgagg gcctcatcgt cccctacctg cgcgtgatcg agcctcggcg
     2581 ctgcactcag gaggccccgt gcccgggccg cgtggccaag gtcaccgtcc ccggcatcac
     2641 cgacgccgac ttcgaagcga tcgcccatct cggcggagat cgcttcctga tcgccagcga
     2701 cgaccggatc gccggtcggt tccgtaccgt cttcgcactg attgccgtcg ccgggctgga
     2761 ttagaaaccg aaggggttca tccgagcgga gcacgcgcca cgggcacgac attggtggta
     2821 ggctttcgcc gaggagaagc cacatcatgg aacgccagat gatccaacac acccgcaccg
     2881 gcaagggccg cccgccaatc gtcctcgtgc acgggttcgg ctgcgcgcgg agcgactggg
     2941 acctgcaggt cgcccatctc gcgcagcact ccgagacgat cgccgtcgat ctcggtggcc
     3001 atggcacgac gccagggacg agcgcgcacg cgcggctcga gacgcatggc gccgacgtcg
     3061 ccgagttgct gcgcggcctc gatctccgtg gcgccgtgct catcggccac agcatgggct
     3121 gccgcgtgat catggaggca gcggcgcgcg agccggcccg ggtcgcgggc gtcattctcg
     3181 tggatggcag ccgcatgggc caggcaggag tgaacgccca caccccactg cttgccgaaa
     3241 tcaaggcgaa gggcggctat gcgagctaca ttcgccctgt attcgagagc atgttcgcca
     3301 agacgtatcc ggcggacaag gccgcgccga tcgtcgagcg cgcggcacgc atgccggccg
     3361 agatcgccgg cccgctgttc gccgacatcc gccgctacga cagcgagcac atggaccgca
     3421 ttctgtctct gcagcgttgg ccattcctcg caatccagac gacacgcatc gaccagggcg
     3481 gcaaccgcgt ctcgatgaag agcggcgaga cgacgccgta tctcgatctc atccgctcga
     3541 aggtggccgg agcccgcatt gagatcattc cgtcgatcgg ccacttcccg caggtcgagg
     3601 cgcccgcagc cctcaaccag ctgctcgaac ggttcgtcgt ttcgctgcgg agctgaggca
     3661 atcgctgctc actgatatcg gccaactgat cctttcaccc gaaagccctc gtgcagtcgg
     3721 ccggtatcac ttctcttctt gcgcgcggcc gcgtcatgcc ggaggcgtgc tgcgcatgcc
     3781 gccaaccacg cacgacacga aatcaacaaa ggtcaatcat ttcgtggctc cgtacgaggc
     3841 gctcccatgc gccgagcgac aaccccgagc agatcctcgg cgcgcgtccc ttccgggagt
     3901 cgggcaagga tcggctcgag gcgctggtcg atcagcagca tcgc
//
LOCUS       DAORNH010000367         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_17287_length_3925_cov_2.622222, whole genome shotgun sequence.
ACCESSION   DAORNH010000367 DAORNH010000000
VERSION     DAORNH010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17287_length_3925_cov_2.622222"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PK264_23445"
     CDS             complement(<1..343)
                     /locus_tag="PK264_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78857.1"
                     /translation="MSDKDRIANLEKQFAALKASHDDLVGFHGRTRSDLTVVQGLVKT
                     LSETVATLESTIEEMKRKVDAHQTQLTTHFELIGRTDGDPKECIVVVDNQRHVVLRDS
                     QDRSPSTKFRFA"
     gene            complement(736..2085)
                     /locus_tag="PK264_23450"
     CDS             complement(736..2085)
                     /locus_tag="PK264_23450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRD78858.1"
                     /translation="MAEPSSSPNIVLILADNLGWGELGCYGNAGLRGAETPRIDKLAA
                     EGLRMLNFNVESDCVPTRSALVTGRHPIRTGAMQSIPAGLPQGLLPWEITLGQLLGQA
                     GYATGCFGKWHLGDRPGRYPHERGFDEWYGIPRTSNESMFTSSPGFDPTIVPMPYVMR
                     GRRGEAAQALHVFDLDARRTIDKEIVEHAIDFIERSARQQRRFFAYVPITHLHFPTLP
                     HPDFAGRTGAGDFADSVAEMDFRVGQIIDALDRLNLAQDTLLMFGSDNGPEFRRPWRG
                     TAGPWTRTYHTAMEGSLRVPFMFRWPGRIAAARTSNEIVHVTDLFPTLARLVGARVPS
                     DRPIDGLDQLDFLLGKTSKSAREGFLFYIKDELRAVKWRHFKMHLILEREPNDGPIKL
                     ESPMIFDLIQDPKEETDAGLAANWVRTPIRYLMQEFQQSLKAHPPIPPGASDDYQPK"
     gene            complement(2789..>3925)
                     /locus_tag="PK264_23455"
     CDS             complement(2789..>3925)
                     /locus_tag="PK264_23455"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD78859.1"
                     /translation="VAGNMVSLQLADFGAEVVKVEDPGRGDTLRAWKEKGISVHWKVY
                     ARNKKSITLNLKSPEAPAVIARLVEGFDVLIESFRFGYLEKLGVGPEALQKINPKLVV
                     VRVSGFGQTGPYAKRPGFGTLVEAMSGFADRNGFEDREPVLPPLAMADMIAGLYGAFA
                     TVMAVRDIEKNGGRGQVIDLSLLEAMNSILGPEAAIHRLSGRRRRRVGSASEQSSPRN
                     VYATSDDRFVAISASTQAMAERLFQAIGRADLNTNPEFASNTERLKRREEVDRIVGGW
                     IKARPLTEAIAHFEKEGVTAAPVYDPGEFLADRHVQERGIVVDLPDDEMGAVPMHDVV
                     PRLSGTPGRLRRPAPKLGEHTSEVLERAGYTASAIAGLRERKVV"
BASE COUNT          717 a         1237 c         1234 g          737 t
ORIGIN      
        1 aggcgaaccg aaacttggtg cttggactcc gatcctggct gtcgcgaagt acaacgtggc
       61 gttgattgtc cacgaccaca atgcactcct tggggtcgcc gtctgtgcgg ccaatcagtt
      121 cgaaatgagt tgtcaattgg gtctggtggg cgtcgacctt tcgtttcatt tcttcgatgg
      181 tcgactctaa cgtagccacc gtctccgaca gcgtcttcac aaggccttgc actactgtca
      241 ggtcggaacg cgtgcggcca tgaaatccaa ccaagtcatc gtggctcgcc ttgagggccg
      301 cgaattgctt ctcaagattt gcaatacgat ccttatcact cattgcaaac actcctccca
      361 gctttagatg cccttaccgt ttcggtgggc gcgcaaagtc aactgactgc gcaccgatga
      421 gttggaaacc cgattgcggc ggaaaaaggt gccgagaggt gacggcctga tgaacggcca
      481 tgcagccatg tttgacattt cgcgtccgaa ttgaagatca tcgaatgatc gcaacagccg
      541 tggaaatcct ctgagcaggc atgagaacct tccgcggggc aggggctaga aaaaaattaa
      601 attggtagtt cccatcgcgc cagtgcagac aacatcgctg ccgattttcc actctgccgg
      661 gtggttcccc gagctaaaga atcgcagaac gcaccatcgc ctccggctgc attcgccgtt
      721 tcgagcgccg cacgcctact tcggctggta atcgtcggag gcgcccggcg ggatcggagg
      781 atgcgccttc aggctctgct ggaactcctg catcaagtac cgaatgggcg tgcgcaccca
      841 gttggccgct agccctgcgt cggtctcctc cttcggatcc tggatgagat cgaagatcat
      901 ggggctctcg agcttgatcg gtccgtcgtt gggctcgcgc tccaggatca ggtgcatctt
      961 gaagtggcgc cacttcaccg cgcgcagctc gtccttgatg tagaagagga agccctcccg
     1021 cgccgacttg cttgtcttgc ccagcaggaa atcgagctga tccagtccgt cgatcggacg
     1081 gtcggacggc accctggcgc caacgagtcg cgcaagtgtc ggaaaaaggt ccgtcacatg
     1141 cacgatttcg ttgctggtac gggcggcggc gatgcggccc ggccaccgga acatgaaggg
     1201 gacgcgcagc gagccctcca tcgccgtatg gtacgttctc gtccatgggc ccgccgtccc
     1261 gcgccagggg cgacggaact cgggtccgtt gtcgctgccg aacatgagca gcgtatcctg
     1321 cgcgaggttc agtcggtcga gcgcatcgat gatctggccg acacggaaat ccatctccgc
     1381 gacgctgtca gcgaagtcgc cggctccggt ccggccggcg aaatccggat gcggcagcgt
     1441 cggaaagtgc aagtgggtga tcggcacgta ggcaaagaag cggcgctgct gacgggcgct
     1501 gcgctcgatg aaatcgatcg cgtgctcgac gatctccttg tcgatcgtgc gccgggcgtc
     1561 gagatcgaag acgtggaggg cctgcgctgc ttccccccgc cgaccccgca tcacataggg
     1621 catcggcacg atcgtcggat cgaaccccgg tgacgaagtg aacatgctct cgttcgaggt
     1681 acgtgggatg ccataccact cgtcgaagcc gcgctcgtgc ggatagcggc ccggccgatc
     1741 gccgaggtgc catttgccga agcagcctgt cgcgtagccg gcttgtccga gaagctggcc
     1801 tagcgtgatc tcccagggca gcaagccctg cggcagtcca gccggaatgg actgcatggc
     1861 acccgttcgg atcgggtgcc ggccggtgac gagagcggat cgcgtcggaa cgcagtcgct
     1921 ctcgacattg aagttgagca tccgcaggcc ttcggctgcg agcttgtcga tgcgtggcgt
     1981 ctcggcaccc cggaggccgg cgttgccgta gcagccaagc tcaccccatc cgaggttgtc
     2041 ggcgagaatg agcacgatgt tgggcgagga ggatggctca gccaattgta tcgcttcctt
     2101 tgatcaggcg ttgcgcgtcg gtgagccacg catcatgatc aggtgccgac atctgcgctc
     2161 ccaagaagta tggacccgtg caatgtcagc tccggagtga ctactagcaa acgatcagcc
     2221 accgtggcta cgcctcagag ctgcagtcgc cggtcacgga ccagagtttc cgtccgtgcg
     2281 ctcggtatcg gagccgcgta tgcgggccac tcggcggcat cgcccccatt gtcgacgcaa
     2341 gggggcgcat cgcctgcaga tgctgttgca agaattggag gcggtcgagg tttcgaacgc
     2401 aggcaatgga ggggagggat ttctgaactg gaggcagagg tcgcgaagct gcgctccggt
     2461 ggatgcccaa tgagctccgc caacggcact cgaacttgct ccgattacga cagtcaccaa
     2521 gtgcgcagca acgagcgacg cgcgcatggt gagctcgtca tgggtgccct ttggcggata
     2581 gtcgacgcca tacgcccatg catcgagagc gcgccagggg gtgatctggt cgtcgagctg
     2641 cctcgccaca gggccagcga catcgcggaa gaccgacgtg atcaccgaga atagcacatt
     2701 tgccattcgc gccggcatga cgctcggcat gacgatcgag gcaaccgagt gcgagttggc
     2761 ggcgacccaa tgacggcagc agtcgggctc acacgacctt gcgctccctg aggccggcga
     2821 tcgccgaggc cgtgtaccct gctcgctcca gaacttcgct cgtatgctcg ccgagcttcg
     2881 gggccgggcg gcggagacgg ccgggcgtgc cactcagtcg tggcaccacg tcatgcatcg
     2941 gcaccgcccc catctcgtcg tcggggagat cgactactat tccgcgctcc tgcacatggc
     3001 ggtcggcgag gaactcgccc ggatcataga ccggcgccgc agtgacgccc tccttctcga
     3061 aatgcgcgat cgcctctgtg agcggccgcg ccttgatcca gcccccgacg atgcgatcca
     3121 cctcctcgcg gcgcttcagg cgttccgtgt tgctcgcaaa ttcgggattg gtgttgaggt
     3181 cggcccgccc gatcgcctgg aagaggcgct ccgccatcgc ctgcgtcgat gccgagattg
     3241 cgacgaagcg gtcgtcgctc gtcgcataga cattgcgcgg cgagctctgc tctgaagcac
     3301 tgccgacgcg ccggcgccgc ctgccggaga ggcgatggat cgccgcttcc ggcccgagaa
     3361 tcgaattcat ggcttcgagc agcgagaggt cgatcacctg gccgcgcccg ccgttcttct
     3421 cgatgtcgcg gacggccatg acggtcgcga aggccccgta tagccccgca atcatgtccg
     3481 ccatggcgag tggtggcagc accggttcgc gatcctcgaa gccgttgcgg tcggcaaagc
     3541 ccgacatcgc ctcgacgagc gtgccgaagc ctggccgttt ggcatagggc cccgtctggc
     3601 cgaagccgga aacacgaacc acgacgagct tcggattgat cttctgaagc gcctccggcc
     3661 ccaccccgag cttctcgaga tagccgaagc ggaagctctc gatcagcaca tcgaagcctt
     3721 cgacaagcct tgcaatgacc gccggcgcct ccggcgactt caggttgagc gtgatgctct
     3781 tcttgttgcg cgcatagacc ttccagtgga cactgatgcc cttctccttc caggcgcgca
     3841 gcgtgtcgcc gcggccgggg tcctcgacct tcacgacctc ggcgccgaag tcggcgagct
     3901 gcaggctcac catgttgccg gcgac
//
LOCUS       DAORNH010000368         3906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_17400_length_3906_cov_2.964944, whole genome shotgun sequence.
ACCESSION   DAORNH010000368 DAORNH010000000
VERSION     DAORNH010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17400_length_3906_cov_2.964944"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..1203)
                     /locus_tag="PK264_23460"
     CDS             complement(448..1203)
                     /locus_tag="PK264_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78860.1"
                     /translation="MGAGAGRLDEEEGCEARARQPEDEHRDAGERRPRKARKALRPAP
                     DEGDALRQHEGESLGDRGGRERGDERGHLQHGDGKPIERARQDADREPRADRQQHAVA
                     RMNDRPSYDGREARYVGDREIELGGQQCEREADRQDGEQRRLLADVQEVGGAREIRRH
                     DEEEADQRRTGGKSAKAFEVCCHSNTCETPTRLQPAPAPKGDSSLSRALMRGTPDRTR
                     HVGSGPTKIPPSCSGDRRAWREGRVQGRGIGRR"
     gene            1340..1582
                     /locus_tag="PK264_23465"
     CDS             1340..1582
                     /locus_tag="PK264_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78861.1"
                     /translation="MAGDYFLYAIAAVLLGMTMFEPGRANIPGTLVAALVLKALGNGL
                     VLMGAAYYWQDIILGVIMVASVALSSVFLRKAAFKV"
     gene            1604..2557
                     /locus_tag="PK264_23470"
     CDS             1604..2557
                     /locus_tag="PK264_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRD78862.1"
                     /translation="MISLKKAAMAIAIAAIGSFAGPLSAAAFELGVVAFQMSADTHAR
                     VANAAAAAAKAKGWSVTQLNSEGALPKHAEQLDALIAKKVSAIVVAMGKPVEAEAQLK
                     AAKEKGIPVITVMSGTSAHTILDVQVNEYKVGAEAALYLLGSIGYSGNILTQRFEGNV
                     GTRIRGRVLDAVLAENQAVKEIGKHTMARTQSWRDDVRAGFQALLLKNQGKVNAIWAS
                     FDGQAFIIDDLLKAAGVKKGQIPLVSIDGGPEAYRRIADPTSTLMATVMIPFEDMGAA
                     AVEAVETIVVKKQPRSAVTAGPYLYKDAILVDASNVAKFVK"
     gene            2626..>2927
                     /locus_tag="PK264_23475"
     CDS             2626..>2927
                     /locus_tag="PK264_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78863.1"
                     /translation="MRWLFLRVLIVRLRAAALTALVVGGLLLLAAGDAAAQETKRPDW
                     SAERKRIEAAVTALAKKIVTPSLVECRMPDGSASEHQRIHVLGFDQDHQLIDAEES"
     assembly_gap    2928..2940
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2941..3242)
                     /locus_tag="PK264_23480"
     CDS             complement(<2941..3242)
                     /locus_tag="PK264_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78864.1"
                     /translation="MGDGIECQLGRPQGFRWQRDPVDSLGDVAIASPVMRDDLDRNSI
                     PIAARAPENQTREVELGDLPFHRSDRLGPLESRAELSRDRLPVEWKPLEFGGAKAFR"
     gene            3672..>3906
                     /locus_tag="PK264_23485"
     CDS             3672..>3906
                     /locus_tag="PK264_23485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78865.1"
                     /translation="MWLLAFALLALIARSYWPEAPTILESRREELPKNIEGSPAALLQ
                     ARYQHKLIEFDDASGHAAEFVSWATTRDATPRGR"
BASE COUNT          630 a         1289 c         1292 g          682 t
ORIGIN      
        1 gagccggttg ttgttcggca gatagacgcc ctcgggcggc acattggcgt gcttcacccg
       61 cgcgccgttg cgggccgccg gcggcgttgc gagcggaggt cgcgggacca tggcgctcgc
      121 cagattatga tcggctccac cagcgcccgc tcgggaaggc agggcgacct cgcgcttcag
      181 gcaggacatg cgctatgctc ctcggatcag cccgccaaag ctaacaagcg ccagcccgga
      241 ccgccagtat gcgcttgtgc ggcatgtgag agcggggccc agcccgtcgc cttgaggcgg
      301 gcctcgtggc gaatctcatt cgttcagagg acgactgctt cgctgtccag gcctaacagt
      361 ttcaacggtg atggtcgctg cctgattgcg gactgtggat tgaggaactc cggattgccg
      421 taacgtatcg attgagtgca gcgatcatca tctgcgtcct atcccccttc cctggacccg
      481 accttcccgc catgccctcc gatctccgga gcagcttggc gggatttttg ttggtccgct
      541 gcccacgtga cgtgtgcgat caggtgtccc gcgcatcaga gcccggctca ggctagaatc
      601 gcccttcggc gccggcgctg gttgcaggcg ggttggggtc tcacaggtat ttgaatggca
      661 gcacacctcg aacgctttgg cactcttgcc gccggtgcgg cgctgatcgg cttcttcctc
      721 gtcatggcgc cgaatttcgc gagccccgcc aacctcttga acatcggcaa ggagacggcg
      781 ctgctcgcca tcctggcgat cggcttcgcg ctcgcactgc tgaccgccga gctcgatctc
      841 tctgtcgccg acgtagcgag cctcgcggcc gtcgtaactg gggcgatcgt tcatacgggc
      901 cacagcgtgc tgctggcggt cggcgcgggg ctcgcggtcg gcatcctggc gggcgcgctc
      961 aatgggcttg ccgtcaccgt gctgaaggtg ccctcgctca tcgccacgct cgcgaccgcc
     1021 gcgatcgcca agggattcgc cttcatgctg acgcaaggcg tcgccttcgt cgggcgctgg
     1081 cctgagagct ttacgggcct tgcgcggtcg tcgctcgccg gcatcccggt gctcatcctc
     1141 tggctggcgg gcgcgggcct cgcagccctc ctcctcgtca aggcgacccg caccggcgcc
     1201 cacatgaccg cgaccggaga agccgaggac gcggcccgac tcgctggcat cgccacgggc
     1261 cgcatgaagc gtttgggcct cctgctcgcc gggctctatg cggcggtgac ggcgatcctg
     1321 ctatcggcga gcccccaaca tggccggcga ctatttcctc tacgccatcg ccgccgtcct
     1381 gctcggcatg accatgttcg agccgggccg cgccaacatc cctggcacgc tcgttgccgc
     1441 cctcgtcctg aaggcgctcg gcaacggcct cgtgctgatg ggggccgcct actactggca
     1501 ggacatcatt ctcggtgtca tcatggtggc gtcggtcgcg ctttcgtccg tgttcctgcg
     1561 caaggcggcc ttcaaggtgt gaagctttga tcggggaaag aggatgatca gtctcaagaa
     1621 ggcggcgatg gccatcgcca ttgccgcgat cgggtcgttt gccggtccgc tgtcggcggc
     1681 agcattcgag ctcggcgtcg tcgcgttcca gatgtcggcc gatactcacg cgcgcgtcgc
     1741 caacgccgct gccgccgccg ccaaggccaa gggctggtcg gtgacgcagc tcaactcgga
     1801 gggcgcgctc ccgaagcacg ccgagcagct cgacgcgctg atcgcaaaga aggtgagcgc
     1861 catcgtcgtc gccatgggga agcccgtcga ggcggaagcg cagctgaagg ccgccaagga
     1921 gaagggcatt ccggtcatca cggtgatgtc ggggacgagc gcacacacca tcctcgacgt
     1981 gcaggtgaac gaatacaagg tcggcgcgga agccgccctc tacctgctcg gcagcatcgg
     2041 ctactccggc aacatcctca cgcagcgctt cgagggcaac gtcggcacgc gcatccgcgg
     2101 ccgcgttctc gacgccgtgc tcgccgagaa ccaggcggtg aaggagatcg gcaagcacac
     2161 catggcgcgg acgcagtcct ggcgcgacga cgtgcgcgcg ggcttccagg cgctgctgct
     2221 caagaaccag ggcaaggtga atgccatctg ggcctcgttc gacgggcagg ccttcatcat
     2281 cgacgacctc ttgaaggccg ccggcgtgaa gaagggccag atcccgctcg tctccatcga
     2341 cggcgggccc gaggcctacc ggcgcatcgc cgatccgacg tcgacgctga tggcgaccgt
     2401 catgatcccc ttcgaggata tgggcgccgc cgcggtcgag gccgtcgaga cgatcgtcgt
     2461 caagaagcag ccgaggagcg ccgtcaccgc cgggccgtat ctctacaagg acgcgatcct
     2521 ggtggatgcc agcaacgtcg cgaagttcgt gaagtgaggg gtgggccccc ggcgtgtgtc
     2581 gccgggggca catcgccgcc gtcgaatgcc tgctagattg cgccgatgcg ttggctgttt
     2641 ctgcgtgtct tgattgtgag actgcgtgcc gccgcgctca ccgcgttggt ggtgggaggg
     2701 ttgttgctgc tcgcggcagg tgatgccgca gcgcaggaga cgaagcgccc cgactggtcc
     2761 gccgagcgca agcggatcga ggctgcggtc acggcgcttg caaagaagat cgtcacgcca
     2821 tcgctcgtcg agtgccgaat gcccgatggc tcggcatcgg aacaccaacg catacacgtg
     2881 ctcggcttcg accaggacca tcagttgatc gacgccgaag agagcgannn nnnnnnnnnn
     2941 cggaaggcct tcgcgccgcc aaactccaag ggcttccact cgacgggcaa gcggtcgcgc
     3001 gaaagttccg ctcgcgattc gagggggcca aggcggtcgc tgcggtggaa ggggagatcg
     3061 ccaagctcaa cttcgcgcgt ctggttttcg ggcgcgcgcg ccgcaatggg gatcgagttg
     3121 cgatcgagat catctcgcat gaccggcgag gcgattgcta cgtctccgag actatcgacg
     3181 ggatcgcgct gccacctgaa gccttgcgga cgcccgagct ggcactcaat gccatcgccc
     3241 attggttcgt cgagggacga gacacacacc gtcccatcat tgcgacggcc gtcaccaacc
     3301 tgccagctgc gtgcagcgac acagtgcgtt cactcgtggg ggattggggc gcggctgtca
     3361 ggagggcgct accggaaaag ggtgccaccc atcggctggt gagcagccct ccgcgacctt
     3421 ggaccgccga aatcaacgtt tcgcggtcgc ggccgcacag catcgatgtg accttgaaga
     3481 gttccgtcgt cgagccgact gatggcgagc agatatttca gagccgcgcc ctcccaatca
     3541 gcctcgctgt gcccgacgcg tgtttcgcgc cgccgccttc gccaccagcc gacccgccat
     3601 gctggcacac gccgtggaac tgcccgttcg acgcgcggct gcagcccatc gagcgggttg
     3661 gatcggtcgc catgtggctg ttggcgttcg ctttgctagc cctcatcgcg cggagctatt
     3721 ggcctgaggc tccaaccatc ctcgaatcga ggcgcgagga gctgccgaaa aatatcgaag
     3781 gctcgccggc ggcgctgctg caggcgcgct atcagcacaa gctcatcgag ttcgatgacg
     3841 cgagcggaca tgcggccgag ttcgtgtcat gggcgacgac cagagacgca acgccgcggg
     3901 gccggc
//
LOCUS       DAORNH010000369         3853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_17750_length_3853_cov_2.332807, whole genome shotgun sequence.
ACCESSION   DAORNH010000369 DAORNH010000000
VERSION     DAORNH010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17750_length_3853_cov_2.332807"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..>360)
                     /locus_tag="PK264_23490"
     CDS             complement(171..>360)
                     /locus_tag="PK264_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD78866.1"
                     /translation="VSLDEVDAQGMLLRKPGVFVAGEMLDFDAPTGGYLMQACLATGK
                     AAGEGCISFLRQLTNASA"
     assembly_gap    361..384
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<385..1435)
                     /locus_tag="PK264_23495"
     CDS             complement(<385..1435)
                     /locus_tag="PK264_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HRD78867.1"
                     /translation="MADKKTVAVVGGGPAGLMAAEVVARAGVGVTVYDRMPSLARKFL
                     MAGRGGLNLTHSEPLEAFLGRYTPAEGPIVEAVRAYPPEAVRAWCEALGQPTFVGSSG
                     RIFPKAMKASPLLRAWLQRLSSAGVEVRTRHRWTDFGPEGKLYFDTPQESVEVRSDAV
                     ILALGGASWPRLGSDGGWQSTLAGLGVTLAPLQASNVGVLVRWSDHFATRHGGKPLKR
                     IAVRLEHGGWQRGELVVTRSGLEGGAVYALGPALRRALAADGRARLTIDLKPDMTDAD
                     VAARLARPRGKRSLANHLRKSLGLEPQAIALMTEARLRSGPGEAGAAHVATGAVKSVA
                     VEVHGLAGLERSISTA"
     gene            complement(1475..1915)
                     /locus_tag="PK264_23500"
     CDS             complement(1475..1915)
                     /locus_tag="PK264_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD78868.1"
                     /translation="MRYLHTMIRVTNIEQSLDFFVNKLGMKEVRRVENQAGRFTLIFL
                     AASEDAARGAAETSPLVELTYNWDPEAYGGGRNFGHLAYEVDDIYALCAKLQKSGVTI
                     NRPPRDGRMAFIRTPDNISIELLQKGDPLPKAEPWASMANTGVW"
     gene            complement(1915..>2167)
                     /locus_tag="PK264_23505"
     CDS             complement(1915..>2167)
                     /locus_tag="PK264_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78869.1"
                     /translation="ALETAEWRALRAAEPGLTGGIDERIALTLPAIFDGKTLISVPPL
                     GWTAPGTDGTGGVTVTCCLADRLGAPCSDNRHPAPRSTA"
     assembly_gap    2168..2235
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2236..>2577)
                     /locus_tag="PK264_23510"
     CDS             complement(<2236..>2577)
                     /locus_tag="PK264_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78870.1"
                     /translation="AEAAIDQATDELIADAVRVDHYGAARIGLDQLRRAAAEICGRLV
                     SRVVQAVRGAAWPPRLARVEGVVRAICARKAGPRLSLGGTVLSLDEASRTITVVREVR
                     ASVGAGFVYPIA"
     assembly_gap    2578..2663
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2664..3328)
                     /gene="tilS"
                     /locus_tag="PK264_23515"
     CDS             complement(<2664..3328)
                     /gene="tilS"
                     /locus_tag="PK264_23515"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRD78871.1"
                     /translation="MARPANVAPPLTMAEVASQFERLLVPPIALAVSGGSDSMALLLL
                     AAEWTRSRGAAGEVAPLTAITVDHGLRAESAAEAEFVAAKCASLAIPHTILRWTGPKP
                     TTGLPEAARQARYDLIAEHLAADARAHTGRPQRRLATAHHRDDVAETFLMRLARGSGL
                     DGLAAMAESTTWPPCGGRLPGRPLMTIVRPLLSVPKDHLIATLAARGCGWHEDPTNRD
                     SSF"
     gene            complement(3338..>3853)
                     /gene="ybgF"
                     /locus_tag="PK264_23520"
     CDS             complement(3338..>3853)
                     /gene="ybgF"
                     /locus_tag="PK264_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HRD78872.1"
                     /translation="DPIAKMLKEQPRPAAAVPPPATTAPATGDRPAGPLPPAAATAAG
                     NPRQLYEVAYAFLLQQDYAGAEASFLEFLARYPSDALAGSAAYWLGEAYFVRGMYQQA
                     ARSFLKGYQTYKASPKAPDSLLKLAMSLDHLGQRGDACATYAELAQKFPQAPQNVKNR
                     AQTERSRLGCT"
BASE COUNT          564 a         1276 c         1255 g          580 t
ORIGIN      
        1 gaaactatgg tcttgttcac tgcccacgcc ctcgtccgga ccggcccctc acccaacgcc
       61 gccgatcatg gagcgacggc tcgatcgcac gcccggacgc gcagcacaag tagtcggaag
      121 caactggaac aactctttga tcagcagagt tcaaattccg gttgggtgcc tcatgccgag
      181 gcgttggtga gttgcctgag aaacgaaatg cagccctcgc cggctgcctt gcctgtcgcg
      241 aggcacgcct gcatgagata accgcccgtc ggagcatcaa aatcgagcat ctcaccggcg
      301 acgaagacgc cgggcttgcg caacagcatg ccctgagcgt cgacctcgtc gagcgacacc
      361 nnnnnnnnnn nnnnnnnnnn nnnncggccg tcgagatgga gcgctcgagc ccggcgagac
      421 catggacctc gaccgcgacg gactttacgg ccccagtggc gacgtgcgcc gcgcctgcct
      481 cgcccgggcc ggaccgaagc ctcgcctcgg tcatgagggc gattgcctgc ggctcgaggc
      541 caagcgactt gcgcagatga ttggcgagcg agcgcttgcc gcgaggtcgc gcgagacgtg
      601 ccgcgacatc cgcgtcggtc atgtcgggct tgaggtcgat cgtgagccgc gcgcgcccgt
      661 ccgcggcgag cgcacgcctg agcgccggac caagcgcata gaccgcgccg ccctcgagcc
      721 cgctgcgcgt gacaacgagc tcgcccctct gccagccgcc gtgctcgagc ctgacggcga
      781 tgcgcttcag gggcttgccc ccatgccgtg tcgcgaaatg atcggaccag cggacgagca
      841 cgccgacatt cgacgcctga agcggcgcaa gcgtcacgcc tagccccgcg agtgtcgact
      901 gccagccgcc atcgctgccg agccgcggcc agctcgcgcc gccgagagca aggatgaccg
      961 cgtcgctacg aacttcgacc gattcctgcg gcgtgtcgaa gtacagcttg ccctcgggtc
     1021 cgaagtcggt ccagcggtgg cgtgtccgca cctcgacacc ggcgctgctg agccgctgca
     1081 gccatgcccg cagcagcggc gacgccttca tcgccttcgg gaagatgcgg ccgctcgagc
     1141 cgacgaaggt cggctggccg agggcctcgc accaagcgcg cacggcctcc ggcgggtaag
     1201 ccctcacggc ctcgacgatc gggccttcag ccggcgtata gcggccgagg aacgcctcga
     1261 gcggctcgct gtgcgtgaga ttgagcccgc cgcgaccggc catcaggaac ttgcgagcga
     1321 ggctcggcat gcggtcgtag acggtcacgc cgacgcccgc gcgcgccacg acctcggccg
     1381 ccataaggcc cgcagggccg ccgccgacca cggcgaccgt cttcttatct gccatatagc
     1441 ctctgtctgg acgggagtgc tcgcgcgccc ggccttacca cacgcccgtg ttcgccatcg
     1501 atgcccacgg ttccgccttg gggagcgggt cacccttctg gaggagctcg atcgagatgt
     1561 tgtccggcgt gcggatgaac gccatgcgcc cgtccctcgg cggacggttg atcgtcaccc
     1621 cggacttctg gagcttggca cagagcgcgt atatgtcgtc gacctcgtaa gcgaggtggc
     1681 cgaagttgcg gccgccgcca tatgcctccg ggtcccaatt gtaggtgagc tcgaccagtg
     1741 ggctcgtttc ggcagcaccg cgggccgcat cctcgcttgc ggctaggaag atgagcgtga
     1801 agcgtccggc ctggttctcg acgcgtcgga cttctttcat gccgagcttg ttcacgaaga
     1861 agtcgagact ctgttcaatg ttggtgacgc ggatcatggt gtgcaggtag cgcatcaggc
     1921 cgtgctcctc ggggcagggt gtcggttatc gctgcatggt gccccgaggc gatcggcgag
     1981 gcaacaagtg acggtgaccc cgcccgtccc atcagtgcca ggcgcggtcc agccgagggg
     2041 gggcacactg atcagcgtct tcccgtcgaa gatcgccggc agcgtcagcg cgatgcgctc
     2101 gtcaatgccg ccggtgagcc ccggctcggc agccctcagc gcccgccatt cggcggtctc
     2161 aagtgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnngcgat ggggtagacg aacccggcac ccacgctggc gcgcacctcg
     2281 cgcacgacgg tgatcgtgcg cgacgcctca tcgaggctca acaccgtccc cccgaggctg
     2341 agccgcgggc ccgcctttct ggcacagatg gcgcgtacga cgccctcgac ccgggcaagc
     2401 cgcggtggcc aggcggcgcc ccgcaccgcc tgcaccacgc gggacacgag tcggccgcag
     2461 atttctgccg ctgcccggcg gagctggtcg agcccgatcc gggccgcccc atagtggtcc
     2521 acgcgcacgg catcggcgat cagctcgtcc gtcgcctggt cgatcgccgc ctcggccnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnntcgaagc tgctgtcccg gttcgtcgga tcctcgtgcc
     2701 agccgcagcc gcgcgctgca agcgtcgcga tcaggtggtc tttcggcacc gagagcagcg
     2761 gccggactat cgtcatcaga ggccggccgg gaaggcgccc gccgcaaggc ggccacgttg
     2821 tgctttcggc catggccgcg agcccatcga gaccgctgcc gcgggcaagg cgcatcagga
     2881 aggtctcggc gacgtcatcg cgatgatgcg ccgttgcgag tcggcgctgc ggtcgaccgg
     2941 tgtgagcgcg cgcatccgcc gcgaggtgct cggcgatcag gtcgtagcgc gcctgccgtg
     3001 ctgcctcagg cagacctgtc gtcggcttcg ggccagtcca gcgcaggatc gtgtgtggga
     3061 tggcgaggct cgcgcacttt gctgcgacga actccgcctc ggcggcgctt tctgctcgca
     3121 gaccatgatc gaccgtgatg gcggtcaaag gtgcgacctc gcccgcggcg ccacggcttc
     3181 gcgtccactc ggctgccagc agcaggagcg ccatgctatc cgagccgcct gagacggcga
     3241 gcgcaatcgg cggcacaagg aggcgctcga actgcgacgc cacctcggcc atcgtgagcg
     3301 gcggcgcgac gtttgccggc ctcgccacga cgaggcctca ggtgcaaccg aggcgcgaac
     3361 gctcggtctg ggcgcggttc ttcacgttct gcggtgcctg cgggaacttc tgcgcgagct
     3421 cggcgtaggt cgcgcacgca tcgccccgct gaccgagatg gtcgagcgac atggcgagct
     3481 tcaggaggct atctggcgcc ttcgggctcg ccttgtaggt ctggtagccc ttcaggaacg
     3541 agcgtgctgc ctgttgatac atgccgcgca cgaaataggc ctcgccgagc cagtaggcgg
     3601 cgctgccggc gagcgcgtcg ctcggatagc gggccagaaa ctcgaggaac gacgcttccg
     3661 cgccggcata gtcctgctgc agcaggaagg cgtaggcgac ctcataaagt tgacgcgggt
     3721 tgccggcagc cgtggcggcg gcgggcggca gcggtccggc tggcctgtca cctgtggcgg
     3781 gtgccgtcgt cgccggcggc ggaactgccg ctgctgggcg cggctgctcc ttcagcatct
     3841 tggcgatcgg atc
//
LOCUS       DAORNH010000370         3789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18175_length_3789_cov_2.640332, whole genome shotgun sequence.
ACCESSION   DAORNH010000370 DAORNH010000000
VERSION     DAORNH010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18175_length_3789_cov_2.640332"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="PK264_23525"
     CDS             complement(<1..99)
                     /locus_tag="PK264_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HRD78873.1"
                     /translation="MIKSELIQKIAASNQHLYHRDVERIVNVIFDEI"
     gene            complement(133..1101)
                     /gene="sppA"
                     /locus_tag="PK264_23530"
     CDS             complement(133..1101)
                     /gene="sppA"
                     /locus_tag="PK264_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HRD78874.1"
                     /translation="MTIETEAVLDRRRLRRRLSLWRVLAVTAGALALGALTYVGAGAP
                     GFGERDQIARVTIDGVIGEDRAQLQMLKRIAEASHVKAVIVFINSPGGTATGGEALYE
                     ALRNVSKAKPVVAQFGTVATSAAYMAGIAADHIVARGNTITGSVGVIWQWPEVTQLLE
                     KLGVKMNAITSGPLKANPNPYQPLDAAGRQVVEQMVAESQKWFLGLVATRRSLATATV
                     PGLEQGRVFTGRDALTLKLIDAIGDEGDAVNWLVTKKGIAKTLKVVDWKPKRDVQFGP
                     WRLAIEAVGRFFGGATGELAASLADDPRVAALRLDGLIALWLPIEK"
     gene            1279..1497
                     /locus_tag="PK264_23535"
     CDS             1279..1497
                     /locus_tag="PK264_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78875.1"
                     /translation="MAKLLRAGAALFVSVLTLPASIAVADIPPCPTRPLSAWFQIGQQ
                     CRTKDGRVCTLIGVLANKQGNWSCRRPG"
     gene            complement(1502..2266)
                     /locus_tag="PK264_23540"
     CDS             complement(1502..2266)
                     /locus_tag="PK264_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78876.1"
                     /translation="MTLPVTPSFRVDGKRALITGGGRGIGKAAGAALAAAGAHVVLAA
                     RSTSEIEEVAAEIQSAGGKATALTLDVLDDRAVQTAVAAAEPFHVLVNNAGINRPQSF
                     VDFKTADLDLIIDLNVRAAFIVAQAVAKSMVATGTKGSLIHVSSQMAFTTGPGRTAYC
                     MSKAALEGLSRCLAAELAPHGIRSNTLAPTFTETPLAKPFLDDPSFMDYVTKKIKLGR
                     VGQVEDLMGALVFLASDASSLMTGTHMLIDGGWTLG"
     gene            complement(2298..3179)
                     /locus_tag="PK264_23545"
     CDS             complement(2298..3179)
                     /locus_tag="PK264_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HRD78877.1"
                     /translation="MRLVPSCLSIVLALLLVACTTTTAKTSAKPALRNGGKVDEIAAV
                     RHLEECRRETQALKEISFDTISGSGPNGAIVHYRVTETSNRRLRKGELFLVDSGGQYE
                     DGTTDVTRTVAIGTPTGEMRRHYTLVLKGHLAVGAARFPRGTPGASIDAFARHALWQH
                     GLDFDHGTGHGVGSYLGVHEGPHGLSKRSLVPLEPGMIVSNEPGFYKEGAYGIRIENL
                     EVVGEPERLPGSDRETMRFETLTLVPYDRRLIDTSLLTAEERSAVDSYHARVRELISP
                     EVGPAFRPWLDAATAPL"
     assembly_gap    3357..3453
                     /estimated_length=97
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3454..>3789
                     /locus_tag="PK264_23550"
     CDS             <3454..>3789
                     /locus_tag="PK264_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRD78878.1"
                     /translation="VPVFLADVKGDFAGLCQAGAMSDRLKARIDQLGIEWSPNANPVV
                     FWDIYGKLGHPVRATVSEMGPTLLGRLLELNDTQQGVLEVVFKCADDEGLLLLDLKDL
                     RSMLSFAAE"
BASE COUNT          614 a         1211 c         1217 g          650 t
ORIGIN      
        1 gatctcgtcg aagatcacgt ttacgatgcg ctcgacgtct cgatgataga gatgctggtt
       61 cgatgcggcg atcttctgga taagctcaga tttgatcata ggccccctcc tgcctaaagt
      121 ctttgttatt cctcattttt caataggtag ccaaagagcg atcagaccgt caagccgtag
      181 tgcggcaaca cggggatcgt cggcgaggct ggcggcgagc tcacccgtcg cgccgccgaa
      241 gaaacgaccc accgcttcga tggccaatct ccagggtccg aactgcacgt cccgcttcgg
      301 cttccagtcg acgaccttga gggtcttggc aatgcccttc ttcgtcacga gccagttcac
      361 ggcgtcgcct tcatcaccga tggcatcgat cagcttcagg gtgagcgcgt cgcggcccgt
      421 gaacacgcgg ccctgctcga gacccggcac ggtcgcggtc gcgaggctgc ggcgcgtcgc
      481 aaccagaccg aggaaccatt tctggctctc ggccaccatc tgctcgacca cctggcggcc
      541 ggccgcatcg agcggctgat aggggttcgg attggccttc agcgggccgg acgtgatggc
      601 gttcatcttg acgccgagct tctcgagcag ctgtgtcacc tcgggccact gccagatcac
      661 gccgacagag cccgtgatcg tgttgccgcg cgccacgatg tgatcggcgg caatccccgc
      721 catgtacgcg gccgacgtcg caaccgttcc gaactgggca acgaccggct tcgcctttga
      781 gacgttgcgc aatgcctcgt agagcgcttc cccgccggtc gccgtgccgc ccgggctgtt
      841 gatgaagacg atcaccgcct tgacgtggct cgcctcggct atgcgcttca acatttgcaa
      901 ctgcgcgcga tcctcgccga tgacgccgtc gatcgtcacg cgggcgatct ggtcgcgctc
      961 gccgaaaccc ggtgcgcccg cgccgacgta cgtgagggcg ccgagtgcga gcgcgccagc
     1021 ggtcacggcg agaacacgcc acagcgacag acgccgccgg aggcgcctcc ggtcgagcac
     1081 ggcctcggtt tcgatggtca tgtcgttacc ccttgtcgcg ccggcagatg cggccgcgcc
     1141 ttcgcatatg tgttggcggc accataagcc tcggcgcggc acggttcaac ggcgtcgctg
     1201 tcgcccgaaa gactcatatg atgcatgctg cagctggcgg cctcgccgtc ggcatcgttt
     1261 gcaagctgga ggtttgccat ggcgaaattg cttcgagccg gtgcagcgct gttcgtctct
     1321 gttcttactc tgccggcctc gatcgcggtg gcggacatcc cgccttgccc gacgcggccg
     1381 ctttccgcct ggttccagat cggccagcag tgtcgcacca aggacggccg ggtctgcacg
     1441 ctgatcggcg tgctcgccaa caagcagggc aattggagct gcaggcgacc gggctgaggc
     1501 ctcagccgag cgtccaaccg ccgtcgatca gcatgtgcgt gccggtcatc aaggacgacg
     1561 cgtccgatgc gagaaagacg agcgcgccca tcaggtcctc gacctggccg acgcggccga
     1621 gcttaatctt cttcgtcacg tagtccatga agctcggatc atcgagaaag ggcttggcca
     1681 gcggcgtctc ggtgaaggtc ggtgcaagcg tgttcgagcg gatgccgtga ggtgcgagct
     1741 cggcggcgag gcagcggctg agcccctcga gggccgcttt tgacatgcag taggccgtgc
     1801 ggccgggccc ggtcgtgaag gccatctgcg atgagacgtg gatgagtgat cccttcgtcc
     1861 cggtcgcgac catgctcttc gcgacggcct gcgccacgat gaaggccgcg cgcacgttga
     1921 gatcgatgat gaggtcgaga tcggccgtct tgaagtcgac gaaactctgc ggccggttga
     1981 tgccggcatt gttgacgagg acgtggaacg gctctgccgc cgcaactgcc gtctgcaccg
     2041 cgcgatcgtc gagcacatcg agggtcagcg cagtggcctt gccgcccgct gactggatct
     2101 ccgcggcgac ctcttcgatc tcggatgtcg agcgtgcggc cagcacgacg tgagccccgg
     2161 ctgcggcaag tgcagcgccg gccgccttgc caatgccgcg gccgccgccg gtgatcaggg
     2221 cgcgcttacc atcgacgcga aacgacggtg tgaccggcag ggtcatggcg gcgtcctttc
     2281 gttgtctgaa atcggtgtca gagcggggcc gtcgcggcgt ctagccaggg cctgaacgcc
     2341 ggcccgactt ccggcgagat cagctcgcgg acgcgggcat gataggaatc gaccgcgctc
     2401 cgctcctcgg ccgtgagcaa gctcgtgtcg atgaggcggc ggtcataggg gacgagcgtc
     2461 agtgtctcga agcgcatcgt ctcgcgatca ctgccgggaa gccgctccgg ctcgcccacg
     2521 acctcgaggt tctcgatccg gatgccatag gcgccttcct tgtagaagcc cggctcgttg
     2581 gacacgatca tgccaggctc gagcggaacg aggctgcgct tcgagaggcc gtgcggaccc
     2641 tcgtgcacgc cgagatagct gccgacgccg tgccccgtgc cgtgatcgaa gtctaggccg
     2701 tgctgccaga gcgcatggcg tgcgaaggca tcgatcgagg cgccgggcgt gccgcgggga
     2761 aaacgcgcgg ccccgaccgc caggtgcccc ttgaggacaa gcgtgtagtg gcggcgcatc
     2821 tccccagtcg gcgtgccgat ggcgacggtg cgcgtcacgt ccgtggtgcc atcctcgtac
     2881 tggccgcccg aatcgacgag aaagagctcg cctttgcgca ggcgccggtt actcgtctcc
     2941 gtcacccggt agtggacgat ggcgccgttc ggacccgacc ccgagatcgt gtcgaaggag
     3001 atctccttca atgcctgcgt ctcgcgccgg cactcctcga ggtggcggac ggccgcgatc
     3061 tcgtcgacct tgccgccgtt gcgcaacgcc ggcttggcgg acgtcttcgc cgtcgtcgtc
     3121 gtacaggcca ccagcaacaa ggcaaggacg atcgacaggc agctcgggac gagtctcatg
     3181 gtcggcacga ttccggacaa aaagcgacgc ggcacggtac ccgacgcacg cgatgcgggc
     3241 aaatgcagcg cagctgcagc cgtcgaccga ctacaatcgg cctgctgtcc cgggcaacga
     3301 ggagacgacg tgaacgacat cctgatcggc aagagcgaca tcgacgtctg gctgcgnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgttccg gtgttcctcg ccgacgtcaa
     3481 gggcgatttc gccgggctct gccaggccgg cgcgatgagc gaccgcctca aggcgcgcat
     3541 cgaccagctc ggcatcgagt ggagcccgaa cgcgaacccg gtggtgttct gggacatcta
     3601 cggcaagctc ggccatccgg tgcgcgcaac cgtttcggag atgggcccga ccctgctcgg
     3661 acgtctgctc gaactcaacg atacccagca gggcgtgctc gaggtcgtgt tcaagtgcgc
     3721 cgacgacgaa ggcctgctgc tgctcgacct caaggacctg cgctcgatgc tgagcttcgc
     3781 cgcggaaaa
//
LOCUS       DAORNH010000371         3783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18210_length_3783_cov_2.194742, whole genome shotgun sequence.
ACCESSION   DAORNH010000371 DAORNH010000000
VERSION     DAORNH010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18210_length_3783_cov_2.194742"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..787)
                     /locus_tag="PK264_23555"
     CDS             complement(119..787)
                     /locus_tag="PK264_23555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD78879.1"
                     /translation="MIVDDLESLGAASDPTVLDIGCGGGLLGARIWQQAIAARAGCYI
                     GVEPDTAAEPAPVFARVHPYTFEEAPIEAASVDLAYAAMVLEHVRQPADFFAKLADVV
                     RPGGVFWAFTVDRRHYFSWCSELLGALRLKDRYLDRVRGRRGENLARYDNFPVYYRCN
                     SPATLSRLAGNEFSLQTWILHRLGQLDGYVPYRLRRLSRMADRIIIRARLPGSVLVAR
                     MVRR"
     gene            complement(954..1763)
                     /locus_tag="PK264_23560"
     CDS             complement(954..1763)
                     /locus_tag="PK264_23560"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRD78880.1"
                     /translation="MAGLESHYSARDIETRILAALRAAGLNPEQGLSPKELAALDHFH
                     TGGLGASRGLLELARIRGEDRVLDIGAGLAGPARLLASTLGCRVDCLEMSRDFCAGAV
                     LLNRLTGLDDRIEVHRGSALDLPFPDDSFDVVWMQNVGMNIADKQKLYVEIYRVLKTG
                     GRFAFQEMAAGEAPTSYFPLPWATDPADSFLVSAEEMGSLLGATGFIVEFLEDTSDAH
                     LSATTANVPPATPGQLSLAVYVDNLAQKAGNARRSLDEGQVRLVRGVFRAQ"
     gene            complement(1859..2500)
                     /locus_tag="PK264_23565"
     CDS             complement(1859..2500)
                     /locus_tag="PK264_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HRD78881.1"
                     /translation="MYLPQHFAETRAEELHRVIREYPLGVLVVDGPRGLDANHLPFEL
                     NPDSGERGHLLAHVARANPLWQEAKDGDEALVIFRAANAYVSPNWYPSKHEFHRQVPT
                     WNYQAVHAHGKIKIRDDERFVRGVVARLTRANEARTGSDKPWKMGDSSKEYIDQMLSA
                     IVGIEIEITQLVGKWKLGQNREERDRINAAEELRKRGEHVTSAAMLAALVNGR"
     gene            2765..>3160
                     /locus_tag="PK264_23570"
     CDS             2765..>3160
                     /locus_tag="PK264_23570"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRD78882.1"
                     /translation="MFNCDLHCHSTCSDGLLPAAEVARRAAANGVDMLALTDHDDVDG
                     LAAAREVADALGMAFVNGVEISIEWERLQIHVLGLAFDVADARLNAGLAAIRSGRIER
                     ARRMSAELALVGVGGAFEGAMRYAANPNLI"
     assembly_gap    3161..3397
                     /estimated_length=237
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3398..3593
                     /locus_tag="PK264_23575"
     CDS             <3398..3593
                     /locus_tag="PK264_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78883.1"
                     /translation="GGQAIEVMSGSHTPEHVAVYGRLAKEYGFLASRGSDFHGPGESY
                     VDLGQLAPLPDGLTPVWEAF"
BASE COUNT          626 a         1102 c         1158 g          660 t
ORIGIN      
        1 gcgcgtcgcc gccggcctgg gaggagtcgc cgcgctgcgt gctggctgtg gtctcccccg
       61 ggcgccccgg agcgcaagcc gggtcgttcg ccagcgcgcg cggtgccagc acgccacctc
      121 accggcgcac catcctcgcc accagcacgc ttcccggaag gcgtgcccgg atgatgatgc
      181 ggtccgccat ccgcgacagc cggcgcaggc gatacggtac gtagccatcg agttgtccga
      241 ggcgatgcag gatccaggtc tgcaaggaaa actcgttgcc ggcgaggcgg ctcagcgtgg
      301 cagggctatt gcaccggtaa tagaccggga aattgtcgta ccgcgcgagg ttctcgcccc
      361 tccgcccgcg cacgcggtcc aggtagcgat ccttgaggcg gagcgcgccc aacagctcgc
      421 tgcaccacga gaagtagtgt cggcggtcta ccgtgaatgc ccagaacact cccccggggc
      481 gcacgacgtc ggcgagcttg gcgaagaagt ccgccggctg gcggacatgc tcgagcacca
      541 tggcggcata ggccagatcg accgaggcag cttcgatcgg cgcctcctcg aacgtgtacg
      601 ggtgcacgcg cgcaaacacc ggcgcgggct cggcggccgt gtcgggttcg acgccgatgt
      661 agcaaccggc gcgcgcggcg attgcctgtt gccagatcct ggcgccaagc aagccgccgc
      721 cgcagccaat gtcgaggacg gtcgggtccg acgcggcgcc aagcgattcg agatcgtcga
      781 cgatcaatcg ggcgtagaac tccccaggac ctcgccaggc gaagccgtat cggccggtga
      841 cgtcctgatc gggcacgcca ggccggaacg gcggtacgtg ggggggcgga tccaggtgag
      901 tcatcgcccg gcaaccggcg atgacggcgc cgcaatctcg gctgtgcgct tactcattgc
      961 gcccggaaga cacctcttac aagccggacc tggccttcgt caagactgcg tcgggcgttc
     1021 ccggccttct gtgcgagatt gtcgacgtac accgccaggc tcagctgtcc cggagtagcg
     1081 ggcggcacgt ttgcggttgt cgcgctcaag tgagcatcgc tggtgtcctc caggaactca
     1141 acgatgaatc cggtcgcgcc gagaagcgag cccatctctt cagcggagac caggaagctg
     1201 tcggccgggt cagtggccca tggaagggga aagtaggacg tcggcgcttc tcctgcggcc
     1261 atctcctgga aagcgaaccg gccccccgtc ttgaggaccc ggtagatctc cacgtagagc
     1321 ttctgcttgt cggcgatgtt catgccgacg ttttgcatcc agacgacatc aaacgagtcg
     1381 tcgggaaacg gcaggtcgag agcactgccc ctatgcacct cgatccggtc gtcaaggccg
     1441 gtcagtcgat tcagcagcac ggcaccggcg cagaaatcgc gcgacatttc gaggcagtcg
     1501 acgcggcagc cgagcgtgga agcgagcagc cgggcgggac cggccagccc ggctccaatg
     1561 tcgagcacgc ggtcttcgcc ccgaattcgc gccagttcga gcaacccgcg tgaggcgccg
     1621 agcccgcccg tgtggaaatg gtcaagcgcc gccagttcct tgggcgacaa gccttgctcg
     1681 ggattcaatc cggcggcgcg gagggccgcc aggattcgcg tctcgatgtc gcgggccgaa
     1741 tagtgtgatt ccagaccagc cataaacagt ccctcctcaa gatccagcgt gacggatcgt
     1801 cggcctggca gtaaatgcag cgaaggtcac cgcccgcaaa aaatggtatt cggcggcatc
     1861 agcgtccatt gaccagagca gccagcatgg ctgcagatgt gacgtgctcg ccgcgcttgc
     1921 gtagctcctc ggcggcgttg atgcgatctc gctcctcgcg gttctggcca agcttccact
     1981 ttccgaccaa ctgggtgatc tcgatctcga tgccgacgat ggctgacaac atctgatcga
     2041 tatactcctt tgatgaatcg cccatcttcc aaggcttgtc ggacccggtg cgcgcctcat
     2101 ttgcccgcgt gaggcgggcc acgacaccgc gcacgaacct ttcgtcatcg cgaatcttga
     2161 tctttccgtg agcgtgcaca gcctggtagt tccacgttgg cacttgccga tgaaactcgt
     2221 gcttgctcgg ataccagttg ggtgagacat aggcattggc agcacgaaag atcacgagag
     2281 cttcgtcgcc atccttcgcc tcctgccata agggattggc gcgcgcgaca tgagcgagca
     2341 gatgtccgcg ctcgccggaa tccggattca actcgaaagg caggtgattg gcatcgagtc
     2401 cgcgcggccc atcaaccacg agcacgccaa gcggatattc gcggatcacg cggtgaagct
     2461 cttccgcgcg cgtttctgca aaatgctgag gtaggtacat accgttccgt tcacaggctc
     2521 atgaaggtta gagtgtaatc aagcttgaac gcggagagaa tgctgcgcat atggctggaa
     2581 cgtcagatgc cgcgtctcgt cgcggccgac tacttctact tgatcagatt gcgtttcaat
     2641 ctgattacgt ctcaggctca taccacgttg gacaaggctg ccgggggagc cgcgcagcgc
     2701 gctgtgctat cataggcagt tctcgatctt gcgctgctca ttctgccctt tcctgtcttc
     2761 tgccgtgttc aactgcgatc ttcactgtca ctctacctgc tctgacggcc ttctcccggc
     2821 tgcggaagtc gcgcgtcggg cggcggcgaa tggtgtcgac atgctcgccc tgaccgacca
     2881 cgacgacgtc gacggcctgg cggcggcgcg cgaggtggct gacgcgctgg gtatggcgtt
     2941 tgtcaatgga gtcgagatct cgatcgagtg ggagaggctg cagattcatg ttctgggcct
     3001 ggctttcgat gtcgccgacg cacgactgaa cgccggtctg gccgctatcc gctcgggtcg
     3061 gatcgaacgt gcgcggcgga tgtcggcgga acttgctctg gtcggtgtcg gtggcgcctt
     3121 cgagggggcg atgcgttatg cggccaaccc gaacctgatc nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgg tgggcaggcg atcgaagtca
     3421 tgtcgggaag tcacacgccg gagcatgtcg ctgtctatgg ccgcctggcg aaggagtacg
     3481 gctttctggc gtcgcgaggt tcggattttc acgggcccgg cgagagttac gtggatctcg
     3541 gccagttggc gcccttgccg gacggcctga cgccggtctg ggaagctttt tgagccttct
     3601 gacaagccat ggcacagtat ctttcaattc atccggagaa cccgcaggcg aggttgctgc
     3661 aaagggcggc cgaggtcatc gggcagggag gggtgatcgc cctgccgact gactcgagtt
     3721 acgctctcgg ttgccagctc ggcgacaagg cggcggtcga gcgcattcgc agtttgcggg
     3781 gtg
//
LOCUS       DAORNH010000372         3777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18247_length_3777_cov_2.576303, whole genome shotgun sequence.
ACCESSION   DAORNH010000372 DAORNH010000000
VERSION     DAORNH010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18247_length_3777_cov_2.576303"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /gene="mltG"
                     /locus_tag="PK264_23580"
     CDS             <1..1491
                     /gene="mltG"
                     /locus_tag="PK264_23580"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRD78884.1"
                     /translation="HIDKREEISLMLKDVIADVRATTKGKQRPQQLAAMERRFYLAGT
                     APAEVVKVAPPTPSEKANPVPEKAAPAAPVPMVKRVQSAPGSDQILTIEPGLQNEQVI
                     AVLLNESRLSGEITKAVPEGTLLPGPYAFKIGDTRQSFLDDLVLAQKLLLRQFWKERA
                     TLLPYKSEREAVILASIVEKEAGRADQREMIAAVFINRLKKGMKLQSDPTVMFGRSSP
                     PDFTRPLTKADIEAKTAYNTYVIDGLPPEPIANPSYESIKAAFNPAATKALFFAPDGA
                     GGHVFSETLKEHQAAVAKWRRIAAAAMSDKAAAMVDKGAVPVRKRVIITLKGIGAGVE
                     SKAAIAAAQDEVIRDLGLTGDAVVRRFDLLPQLVLLLAEEALAKVRAHPRVARVDDDL
                     SQPLPAPVPSPGDVNSPKKAQTLGYVAVLVSRASHLEALKALADLHAAYPDILGGKSL
                     TVVEARIPTGVWHRVIAGPPGSMETAREVCTKLKAKGYSGCFTHGY"
     gene            1656..2951
                     /gene="glyA"
                     /locus_tag="PK264_23585"
     CDS             1656..2951
                     /gene="glyA"
                     /locus_tag="PK264_23585"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRD78885.1"
                     /translation="MTSHSAARDRAFFASPLAEVDPAIHAIIGREMGRQRDEIELIAS
                     ENIVSKAVLDATGSVLTNKYAEGYPGKRYYGGCQFVDEAETLAIERAKQLFGCRHANV
                     QPSSGSQMNQAVFLALLKPGDVFMGLDLNSGGHLTHGSPVNMSGKWFKVAPYGVRRQD
                     CLLDMEEVERIAREAKPKLIIAGGTAYSRHWDFAHFRRIADSVGAILMVDMAHFAGLV
                     AGGAHPSPFPHAHVVTSTTHKSLRGPRGGIILSNDDDIAKKINSAVFPGLQGGPLMHV
                     VAAKAVAFGEALRPEFKAYARQIVDNARALAATLQSHGLDIVSGGTDNHLMLVDLRPK
                     RVTGAAAEKALGRAHITCNKNGVPFDPEKPFVTSGVRLGTPAGTTRGFGVAEFKDIGR
                     MIAEVVDGLGRNGESGNAATEARIRDEATALCRRFPIYP"
     gene            2987..3502
                     /gene="nrdR"
                     /locus_tag="PK264_23590"
     CDS             2987..3502
                     /gene="nrdR"
                     /locus_tag="PK264_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRD78886.1"
                     /translation="MRCPYCQGENTQVRDSRPTDENAAIRRRRSCPDCGGRFTTFERI
                     QLRELMVVKRSGERVPFERVKIERSVGMALRKRPVQPDRVERLVTGIVRQLESSGEGE
                     VRTSEIGALVMEALRGLDPVAYVRFASVYRDFRDAADFRAVLDEIAKAPEARAETGDV
                     PPIVSPEVPRH"
     gene            3502..>3777
                     /locus_tag="PK264_23595"
     CDS             3502..>3777
                     /locus_tag="PK264_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78887.1"
                     /translation="MAHSSDDTALMRIALRMARRGLGTTAPNPSVGAVIADPETGEVI
                     ARGWTQPGGRPHAETEAIRQYRESLPEQVRTLFDRFQFCDLAIKVVGV"
BASE COUNT          656 a         1272 c         1238 g          611 t
ORIGIN      
        1 catatcgaca agcgggaaga gatcagcctg atgctgaagg acgttatcgc cgacgtccgc
       61 gcgacgacga aggggaagca gcgcccgcag cagctcgcgg cgatggaacg gcggttctat
      121 cttgccggga cagcgccggc cgaggtggtg aaggtcgcgc cgccaacgcc atcggaaaag
      181 gcgaatccgg tgccagagaa ggcggcgccg gccgcacccg ttccgatggt caaacgtgtt
      241 cagtctgctc cgggatcgga tcagatactg acgatcgaac cgggcttgca gaatgagcag
      301 gtcattgctg tgctgctcaa cgagagccgc ttgagcggcg agatcacaaa ggctgttccg
      361 gagggcaccc tgctgccagg gccctatgcc ttcaagatcg gcgacacccg ccaatccttc
      421 cttgatgacc tggtgctcgc tcagaagctc ctcctgcggc aattctggaa ggagcgggca
      481 acgctcctcc cgtacaagag cgagcgcgag gccgtgatcc tcgcatcgat cgtcgagaag
      541 gaagcggggc gagccgatca gcgcgagatg atcgccgcgg tcttcatcaa ccgcctgaag
      601 aagggcatga agctccagtc cgatccgacg gtcatgttcg ggcgcagcag ccctccggat
      661 ttcactcggc cgctgaccaa agccgacatc gaagccaaga cggcttacaa tacctacgtc
      721 atcgacgggc tgccgccgga accgatcgcc aatccgagct acgagtccat caaagccgcc
      781 ttcaatccgg ctgcgaccaa ggcgctgttc tttgcgcctg acggcgcagg cgggcatgtg
      841 ttctcggaga cgctcaagga gcatcaggcg gctgttgcga aatggaggcg gattgctgcg
      901 gccgcgatgt cggacaaagc ggcggcgatg gtggataagg gcgccgtgcc ggttcgcaaa
      961 cgcgtcatca tcacactcaa ggggattggt gcgggagtag agtccaaggc tgctatcgct
     1021 gcggcgcagg acgaggtgat ccgcgatctc gggctgactg gtgacgccgt cgtgcgacgg
     1081 ttcgatctgc taccgcagct tgtgctcctc ctcgccgagg aggctctcgc caaggttcgc
     1141 gcgcatccgc gcgtcgcgcg cgtcgatgat gatctcagtc agcccttgcc cgcaccggtg
     1201 ccaagtccgg gagacgtcaa ctcgccaaag aaggcgcaga cactgggtta tgtggcggtg
     1261 ttagtatcga gagcgtcgca tctggaagcc cttaaggcgc tcgcagacct tcatgccgct
     1321 tatccggaca tccttggcgg caagtccctg acggttgtcg aggccaggat ccctactggt
     1381 gtctggcacc gcgtcatcgc cgggccgccg ggaagcatgg agactgcgcg cgaagtttgc
     1441 accaagttga aggccaaggg ctactcgggc tgctttacgc atggctattg aattgggacc
     1501 tgatggacgt tcactccgcg gctccgccca cccccgcacc cgccgcctcg cccgtacttg
     1561 ttgcgctgcc ggtcgcgtgc tatccgcgac cccgtcgcat tcgcagccgc agcatgccga
     1621 cggcaggctt gcggtcgccc gctcgaggac acgccatgac gagccactcc gccgcccgtg
     1681 accgcgcctt cttcgcgtct ccgctcgccg aggtcgatcc ggcgatccac gcgatcatcg
     1741 gccgcgagat gggtcggcaa cgcgacgaga tcgagctgat cgcctcggaa aacatcgtct
     1801 ccaaggccgt cctcgacgcc accggctccg tgctcaccaa caagtatgcc gagggctatc
     1861 ccggcaagcg ctactacggc ggctgccagt tcgtcgacga ggccgagacg ctcgccatcg
     1921 agcgggcgaa gcagctgttc ggttgccgcc acgccaacgt gcagccgagc tccggcagcc
     1981 agatgaacca ggccgtgttc ctggcactcc tgaagcctgg cgacgtcttc atgggcctcg
     2041 acctcaactc gggcggacat ctgacgcacg gctcgcccgt caacatgtcg gggaagtggt
     2101 tcaaggtggc gccttatggc gtccgccggc aggattgcct cctcgacatg gaggaagtgg
     2161 agcggatcgc acgcgaggcg aagccgaagc tgatcatcgc cggcggtacg gcctactcgc
     2221 gccactggga cttcgcccat ttccgccgca tcgccgacag cgtcggcgcg atcctgatgg
     2281 tcgacatggc gcacttcgcg ggcctcgttg ccggcggcgc gcatccttcg ccattcccgc
     2341 atgcccatgt cgtcacctcg acgacgcaca agtcgctgcg cggcccccgc ggcggcatca
     2401 tcctctcgaa cgacgacgac atcgccaaga agatcaactc ggccgtgttc ccgggcttgc
     2461 agggcggtcc gctcatgcac gtcgtcgccg ccaaggcggt cgcgttcggc gaggccctga
     2521 gacccgagtt caaggcctac gcccgccaga tcgtcgacaa tgcgcgggcg ctcgccgcga
     2581 cgcttcagag ccacggcctc gacatcgtct cgggcggcac ggacaatcat ctgatgctcg
     2641 tcgatctccg tccgaagagg gtgacgggtg ccgccgccga gaaggcgctt gggcgcgccc
     2701 acatcacctg caacaagaac ggcgtgccct tcgatccgga gaagccgttc gtcacctccg
     2761 gcgtgcgcct cggcacgccc gccggcacga cgcgcggctt cggcgtcgcc gagttcaagg
     2821 atatcggccg catgatcgcc gaggtggtcg acgggctcgg ccggaacggc gaaagcggca
     2881 acgcggcgac cgaggcacgc atccgcgatg aggcgacggc actctgccgt cgcttcccga
     2941 tctatccctg acacgccatc ccgcgagcga aaggcaaacc cgagccatgc gctgccccta
     3001 ctgccagggt gagaacacgc aggttcggga cagccgcccg accgacgaga acgcggccat
     3061 ccgccggcgg cggagctgcc cggattgcgg cggtcgcttc acgaccttcg agcgcatcca
     3121 gctgcgcgag ttgatggtcg tcaagcgctc gggcgagcgc gtccccttcg agcgcgtgaa
     3181 gatcgagcgc tcggtcggca tggcactgcg caagcgtccc gtgcagccgg atcgcgtcga
     3241 gcggctcgtt acgggaatcg tgcgccagct cgagagcagc ggcgagggcg aggtgcgcac
     3301 gagcgagatc ggcgccctcg tcatggaggc gctgcgcggc ctcgatcccg tcgcctatgt
     3361 gcgcttcgcc tcggtctatc gcgacttccg ggatgcggcc gacttccgcg ccgtgcttga
     3421 cgaaatcgcc aaggcgccgg aggcgcgcgc cgagacgggc gacgttccgc cgatcgtttc
     3481 gcccgaggtg ccgcggcact gatggcgcac tcatccgacg ataccgcctt gatgcgcatc
     3541 gcactccgca tggcgcggcg tggtctcggc acgacggcgc ccaatccgtc cgtcggcgcg
     3601 gtgatcgccg atcccgagac gggcgaggtc atcgcgcgcg gctggacgca gccgggcgga
     3661 cgcccgcatg ccgagaccga ggcgatccgg cagtatcgcg agtcgctgcc cgagcaggtg
     3721 cgcaccctgt tcgaccgctt ccagttctgc gacctcgcca tcaaggtggt gggcgtg
//
LOCUS       DAORNH010000373         3770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18279_length_3770_cov_2.571467, whole genome shotgun sequence.
ACCESSION   DAORNH010000373 DAORNH010000000
VERSION     DAORNH010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18279_length_3770_cov_2.571467"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PK264_23600"
     CDS             complement(<1..552)
                     /locus_tag="PK264_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78888.1"
                     /translation="MSWTIDFAPMIPGPLFWVAVVVAALLVGLLLFRRMRGAVLRALA
                     LASLLTALANPTLRQEERESLANIAIVVTDESASQTLAGRPEQTAAIKADLEGKLKAI
                     PGLQVKWVSSSRPAGDTSGTNLFLDLNRALANVPTDRLAGVIMITDGQVHDIPKSAAA
                     LGFDAPVHALLTGRPDEFDRRIEI"
     gene            complement(595..>1191)
                     /locus_tag="PK264_23605"
     CDS             complement(595..>1191)
                     /locus_tag="PK264_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HRD78889.1"
                     /translation="TDEIAFFPILYWPVLSSVEALPQAILAKIDAYMKNGGMIIFDTR
                     DHGTGVPMELAMRRGGTALQKLIGNLDIPRLEPVPEGHVLTKSFYLIRTFPGRFDGGP
                     LWVEAESSEATSASTTRKARRVDGVSSILITSNDFAAAWAVDDRNQPMFPVVPGGDAQ
                     RELAIRVGINIAMYALTGNYKADQVHIPMLLERLGTSN"
     assembly_gap    1192..1261
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1262..3525)
                     /locus_tag="PK264_23610"
     CDS             complement(<1262..3525)
                     /locus_tag="PK264_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRD78890.1"
                     /translation="MTIGALGFLSPVLLLGLLALPIIYWLLRAVPPRPQQIEFPATRI
                     LVGIENKERTPHKTPWWLVLLRMLAAALVILALAEPVLNPNREQGLKGAGPVVLVVDN
                     GWSAGTRWSERARLVERYINEAEATGRPVLIAGTALASKAQTFKIEPPGAARSTAAAL
                     QPQPFEPNRVEVAKRIREAFGPSAPSGLSVVWLGDGIDHSGEGRVFAETLLALGSGSS
                     VSLVETTAGDEALGILSGVGAAGKLEATVIRAAGGPRAGFVHAFSGRGQRLGEVAFRF
                     FGSEKRTSVAFDIPLEIRNQVTRLEIASERSAGAVHLLDARSQWHRVGLISSESREQA
                     QPLLAPLYYIQKALQPYSELVPTKDATLPAAIETVLKRNVSVMVLADIGTLPGDLRPR
                     IEEFVRKGGVLVRFAGPRLERGGDELIPVELRTGGLTRGGALSWSKPEPLASFSEDSL
                     FVGLEVPADVQVSRQVLADPAKLGPDVKIWARLKDGTPLVTAKKLGEGQMVLFHVTAN
                     SEWSNLPMSGLFVEMLRRLATLGTMSSLGSGDADVADQSRVVSGAGADVLAPSQVLDG
                     FGILRAPPPTAQAIAMKSLATIKPSLDHPPGYYGPQGVPRAFNLIGVKSELRPLSGLP
                     SGIERRAYEGSTQRLLKPDLLTIALSLLLIDVIIVILMQMGNFWRQRLATRGTAALVV
                     ALALGGALLGALDGAKPAAAQGLGQSPQPPGLTPPRSRDEQVAIAATGKVSFAYVITG
                     DRAVDETSRAGLKGL"
     gene            complement(3522..>3770)
                     /locus_tag="PK264_23615"
     CDS             complement(3522..>3770)
                     /locus_tag="PK264_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRD78891.1"
                     /translation="EDGTRWIADRTESLRDRYRAKLIAHRAELEAHAARLGWTFLVHH
                     TDRPASEPLLALIGRLQGAGDNYRWRASPSASKEASRS"
BASE COUNT          577 a         1160 c         1383 g          580 t
ORIGIN      
        1 gatctcgatg cggcgatcga actcgtccgg tcggccggtg aggagcgcgt gcacgggagc
       61 atcgaagccg agggctgcag ccgatttcgg tatgtcgtgc acctgcccat cggtgatcat
      121 gatgacgccg gcgagccggt cggtcggcac gttggcgagc gcgcggttga gatcgaggaa
      181 caggttcgtg ccgctagtgt cgccggcggg gcgcgaggac gagacccatt tcacctgcag
      241 gcccgggatg gccttcagct tcccctcgag gtcggccttg atggcagcag tctgctcagg
      301 gcggccggcg agcgtctggc tggcgctctc gtcggtgacg acgatcgcga tgttggcgag
      361 gctctcgcgc tcctcctggc gcagcgtcgg gttggcgagc gcggtcagga gcgaggcgag
      421 cgcgagggcg cgcagcaccg cgccgcgcat gcgccggaag agcagcaggc cgaccagcag
      481 tgcggccacg accacggcga cccagaacag ggggccgggg atcatcgggg caaagtcgat
      541 tgtccagctc atctctcgaa ctctagcttg tcggagcgct tcggcagcgc tgcctcaatt
      601 gctggtgccg agccgctcga gcagcatcgg gatgtgcacc tgatcggcct tgtagttgcc
      661 agtgagggcg tacatggcga tgttgatgcc gacgcggatc gcgagctcgc gctgggcatc
      721 acccccgggc acgaccggga acatcggctg gttgcgatcg tcgacggccc aggcggcggc
      781 gaagtcgttt gaggtgatga ggatcgaact cacgccatcg acgcggcggg ccttgcgtgt
      841 cgtcgaggca ctggtcgcct cggagctttc ggcctcgacc cagagcggcc cgccgtcgaa
      901 gcggccgggg aaggtgcgga tgaggtagaa cgacttggtc aacacgtggc cttcgggcac
      961 tggctcgagg cgcgggatgt cgaggttgcc gatgagcttt tgcagcgccg tgccgccgcg
     1021 gcgcatcgcg agttccatcg gcacgccggt gccgtggtcg cgcgtgtcga agatgatcat
     1081 gccgccgttc ttcatgtagg cgtcgatctt ggcgaggatc gcctgcggca gcgcctccac
     1141 gctcgatagc acgggccagt agaggatcgg gaagaaggcg atctcatcgg tnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 naggcccttg aggccggcac ggctcgtctc gtcgacggcg cgatcgcccg tgatgacgta
     1321 ggcgaacgag acctttccgg tggccgcgat cgccacctgc tcgtcgcgcg agcgcggcgg
     1381 cgtgagacct ggcggctgtg gcgattggcc gaggccctgg gcagcggccg gcttggctcc
     1441 gtcgagcgct ccgagcagtg cgccaccgag tgcaagcgcg acgacgagtg cggcggtgcc
     1501 ccgcgtcgcg aggcgctggc gccagaaatt gcccatctgc atgaggatca cgatgatgac
     1561 gtcgatcagg agcagcgaca gggcgattgt gaggaggtcg ggcttgagga gccgctgcgt
     1621 gctcccttcg taggcgcgcc gctcgatgcc ggacggaagc cctgagagcg gcctaagctc
     1681 ggacttcacc ccgatcaggt tgaaggcgcg cggtacgccc tgaggcccgt agtagccggg
     1741 cggatggtcg agggacggct tgatcgtcgc gagcgacttc atggcgatcg cctgcgcggt
     1801 cgggggcggg gcgcgcagga tgccgaagcc gtccagcacc tgtgaaggag cgaggacgtc
     1861 ggcgccggca ccactgacaa cgcggctctg gtcggcgaca tcggcgtcgc ccgagccgag
     1921 gctcgacatc gtgccgagag ttgcgagccg gcgcagcatc tcaacgaaga ggccggacat
     1981 cggcaagttc gaccactcgg aattggcggt gacgtggaag aggaccatct ggccttcgcc
     2041 gagcttcttc gcggtgacga gcggcgtgcc gtccttgagg cgcgcccaga tcttcacgtc
     2101 ggggccgagc ttcgccggat cggcgagcac ctgccggctc acctgcacgt cggcgggtac
     2161 ctcgaggccg acaaacaggc tgtcctcgga gaagctggcg agtggctcgg gcttcgacca
     2221 cgagagcgcg ccgccgcgcg tgaggccacc ggtgcgcagc tcgaccggga tcaattcgtc
     2281 gccgccacgc tcgagacggg gaccggcgaa gcggacgagc acgccgccct tgcgcacgaa
     2341 ctcctcgatc cgcgggcgca ggtcgccggg tagcgtgccg atgtcggcga ggaccatcac
     2401 cgagacgttg cgcttgagca ccgtctcgat ggcggcgggc agcgttgcgt ccttggtcgg
     2461 cacgagctcg gagtagggct gcagcgcctt ctggatgtag tagagcggcg cgagcagcgg
     2521 ctgcgcctgc tcccggctct cgctcgagat gaggccgacg cgatgccatt gcgagcgggc
     2581 atcgagcagg tgcaccgcgc ccgccgagcg ctcggaggcg atctcgaggc gcgtcacctg
     2641 gttgcggatc tcgagcggga tatcgaaggc gaccgacgtg cgcttttcgc tgccgaagaa
     2701 gcggaaagct acttcgccga ggcgctggcc gcgaccggag aaggcgtgca cgaaacctgc
     2761 ccgcggtccg cccgcagcgc ggatcacggt cgcctcgagc ttgccggcag caccgactcc
     2821 cgagaggatg ccgagtgcct cgtcacctgc cgtcgtctcg acgaggctta ccgacgagcc
     2881 gctgccgaga gcgagcaggg tttcggcgaa gacgcggcct tcgccgctgt ggtcgatgcc
     2941 gtcgccgagc cagacgacac tgaggcccga gggagctgac ggaccgaacg cttcgcggat
     3001 gcgctttgcc acctcgacgc gattgggctc gaagggttgc ggctggaggg ctgcggcggt
     3061 ggaacgtgcg gcacctggcg gctcgatctt gaaggtttgt gccttcgagg cgagtgctgt
     3121 cccggcgatc agcacgggcc gtccggtcgc ctcggcctcg ttgatgtagc gctcgacgag
     3181 gcgggcgcgc tccgaccagc gcgtgccggc ggaccagccg ttgtcgacga ccagtacgac
     3241 cgggccggcg cccttcaggc cctgctcgcg attggggttc agcacgggct cggcgagcgc
     3301 caggatgacg agcgcggcgg cgagcatgcg cagcagtacg agccaccacg gcgtcttgtg
     3361 gggcgtgcgt tccttgttct cgatgccgac gaggatccgt gttgccggaa actcgatctg
     3421 ctgcggacgc ggcggcactg cgcgcagcag ccaatagatg atcggcaagg cgagaaggcc
     3481 gagcagcagc acgggactga ggaagccgag cgcgccgatg gtcatgagcg tgacgcctct
     3541 ttcgatgcgg atgggctcgc gcgccagcgg tagttgtcac cagcaccctg caagcggccg
     3601 atcagcgcga gcagcggctc ggaggccggc cggtcggtgt ggtggacgag gaaggtccag
     3661 ccgagacggg cggcgtgagc ctcgagctcg gctcggtgag cgattagctt cgccctgtag
     3721 cggtcacgca ggctctcggt gcggtcggcg atccagcgcg tgccgtcctc
//
LOCUS       DAORNH010000374         3739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18496_length_3739_cov_2.386808, whole genome shotgun sequence.
ACCESSION   DAORNH010000374 DAORNH010000000
VERSION     DAORNH010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18496_length_3739_cov_2.386808"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..923)
                     /locus_tag="PK264_23620"
                     /pseudo
     CDS             complement(132..923)
                     /locus_tag="PK264_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
     gene            complement(976..1662)
                     /locus_tag="PK264_23625"
     CDS             complement(976..1662)
                     /locus_tag="PK264_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRD78892.1"
                     /translation="MSGTALKIEPIAEGLSLKARAYDALKSAIMNMNIYASDAELRLD
                     ERDLSARFGISRTPLREALAQLDQEGLVRIVARRGIFIVRRTKAEILDMITVWAALES
                     MAARLATKEASAEELAGLHAHIDAFAGDALAARMGEYSDANIRFHQAIIGLGKCALIV
                     QITDGLFHHVRAIRQRTIFEQDRARRSIVDHMEIVAALEARDTERAERLVREHTLRLR
                     DHVDRYVDLD"
     gene            2087..>3739
                     /locus_tag="PK264_23630"
     CDS             2087..>3739
                     /locus_tag="PK264_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HRD78893.1"
                     /translation="MEEFASLFQGFATALTPFNLVLMFTGILLGVIIGVLPGLGGANG
                     VAILLPLTFSLAKAPGGATSAIILLSCIYWGALFGGAITSILFNIPGEPWSVATTFDG
                     HPMAQQGRADEALTAAFTSSFIGAFVAVLVVTFLAPPIARFAVKFGPPEYFAVFFLTF
                     ASFVGMGKGSAAKTIAAMCLGFALGAVGLDKVTGTLRMTFDQEHLLGGFDFLIAVIGL
                     FGLGEILSSMESGLKFGGTQAKIRLNAVLHTWKKLLAYWVTSIRSCIVGCWMGITPGG
                     ATPASFMAYGLAKRFSPRGDKFGKGEIEGVVAPETAAHAAGTSALLPMLTLGIPGSPT
                     AAVLLGALLMWGLQPGPMLFVEQKDFVWGLIASMYLGNLAGLIVVLTCVPLFAAILRI
                     PFSIIAPMILVICVVGAYTVKNSMFDVWVMLLFGVAGYGFNKLGYPLAPLVLAIILGD
                     KAEEAFRQALLGSQGSLLVFWYDANTGINWLAGSIMTLGLALIAMPMFEWARGLSRRP
                     PPAAAAVAPAAGTGPSSGSASSAAAPSSPAVARASAAVQPVAP"
BASE COUNT          593 a         1196 c         1221 g          729 t
ORIGIN      
        1 gcgggtcagg tcgttcatgc cgtcttcgct aactgtgtct ggccggcctg ggtccgtttg
       61 accgtgatcg ggccgcagga ctcggtctgc tcccaggccg cttcgccctt cttgtagagg
      121 tcgagaatct cgtcgggcag caggcccttc gccatgctca tcggcaggta ggggataccg
      181 gtcttctcga caagcgcacg gatgtcgtcg tcggcttgcg cataggcggc gccctttccg
      241 aggatgatca gcggcttcgt cgcacccttg aggagagcaa gcgcgcgatc gatcgactcg
      301 agcgagggaa tgttgcgcgg cgccggatcg accaccttga tgagcgactt gcggccggcc
      361 tcggcgttca tcgtctgtcc gaagagcttt gccgggaggt cgagatagac gccgccgggg
      421 cgcccagaga ccgccgctcg gatggcacgc gcgatgccga cgccgatgtc ctcggcgtgc
      481 aggacgcgat aggcggcctt cgacaccatc ttggccaccg cgagctggtc catctcttcg
      541 tagtcgccct gctgcaggtc gacgatctcg cgctcggagg agcccgaaat caaaatcatc
      601 ggaaagcagt tcgtcgtcgc atgcgcgagc gcgttgaggc cgttgaggaa gccgggggcg
      661 gacacagtga ggcagacgcc cggcttctgc gtgagaaaac ccgcgatgct cgcggcatag
      721 cccgcgttct gctcgtggcg gaaggcgatg acgcgcatgc ccgccgactg catgtagcgg
      781 ccgaggtccg tgatcgggat gccgggcacg ccatagatgt tcgtgatccc gttgagcttc
      841 agcgcgtcga tgacgaggtg gaagccatcg gtcaggtcct gggtctgggg ctcggacgac
      901 atctgctgtt tctctccctg catgcttttc tgttctggac gcgtctcgtg tcggggcgcg
      961 tcgcggcgaa tgcggtcagt cgaggtcgac gtagcggtcg acgtggtcgc gcaggcgcaa
     1021 ggtgtgctca cgcacgagcc gctcggcccg ctccgtgtcg cgcgcctcca gcgccgcgac
     1081 gatctccatg tgatcgacga tggaacgccg cgcccgatcc tgctcgaaga ttgtgcgctg
     1141 gcggatggcc cggacgtgat ggaagagtcc gtccgtgatc tggacgatga gcgcgcactt
     1201 tccgaggccg atgatcgcct ggtggaagcg gatgttggcg tcggagtatt cgcccatgcg
     1261 ggcggcgagc gcatcccccg cgaaggcatc gatatgggcg tgcaggccgg cgagttcctc
     1321 agcggacgcc tccttcgtcg caaggcgcgc cgccatgctc tcgagggcgg cccacaccgt
     1381 gatcatgtcg aggatctcgg ccttcgtgcg ccggacgatg aagatgccgc ggcgcgcgac
     1441 gatcctgacg agcccctcct gatcaagctg agcgagggcc tcgcggagag gcgtgcgcga
     1501 gatgccgaag cgggcggaca ggtcgcgctc atcgaggcgc agctcggcat cgctcgcata
     1561 aatgttcatg ttcatgatcg ccgacttcaa cgcatcgtag gcgcgagcct tgaggctcag
     1621 tccttcggca atcggctcga tcttgagagc ggttccggac atcgacaagc acgcgtcctc
     1681 gagaaaacag gttgaatgga acatcgcctt cccacttggc acatggcata ccatacgcca
     1741 gtaccatcat caatggtagc tgtgacatgg gacgcatcga ctctgcgaca cagcgattgt
     1801 cggccatttg cctcgccaat cgaaagctgt tcggtgctcc gctgacagat cgaattggtg
     1861 cttgctggca gatgggccct gtgcatatgg tatacctgtt ggtcgttctc tacagatgtc
     1921 ctggcgggcc cgctcatgac ctcgacggac gcggacaatg aatgatccgc acgtgagccg
     1981 gcagtgtcgg gaatgcacct gagacgatag actacacggg gtggaatgca ggcagtagat
     2041 gacaaggccg agaacggccg gcgtcgactg cacggggagg tagagcgtgg aagaattcgc
     2101 gtccctcttt caagggttcg caacggcgct gacgccgttc aatctggtcc tgatgttcac
     2161 cggcatcctg ctcggcgtga tcatcggtgt gcttcccgga ctcggaggcg ccaacggcgt
     2221 cgcgatcctg ctgccgttga cattcagtct cgccaaggcg cccggcggcg cgacttcagc
     2281 catcatcctc ctctcgtgca tttactgggg cgccctcttc ggtggtgcca tcacctcgat
     2341 cctgttcaac atcccaggcg agccttggtc ggtcgccacc accttcgacg gccatccgat
     2401 ggcgcagcag ggtcgcgcgg acgaggcgct gacggccgcc ttcaccagct cgttcatcgg
     2461 cgccttcgtc gccgtgctcg tggtcacatt tctcgcgccg ccgatcgcca ggttcgcggt
     2521 gaagttcggg ccacctgagt atttcgcggt gttcttcctc acctttgcga gcttcgtcgg
     2581 catgggcaag ggcagcgcgg cgaagacgat tgccgccatg tgcctcggct tcgcgctcgg
     2641 agccgtcggg ctcgacaagg tgaccggcac gctgcgcatg acgttcgatc aggagcacct
     2701 gctcggcggc ttcgatttcc tcattgccgt catcggcctc ttcggcctcg gtgagatcct
     2761 ctcgtccatg gagagcggcc tcaagttcgg cggcacacag gcgaagatcc ggctgaacgc
     2821 tgtgctgcat acctggaaga agctgctcgc ctattgggtc acctccatca ggagttgcat
     2881 cgtcggctgc tggatgggca tcacgccggg cggcgccacg ccggcctcgt tcatggctta
     2941 cggtctcgcc aagcgcttct cgccgcgggg cgacaagttc ggcaaaggtg agatcgaagg
     3001 cgtcgtcgca cccgagaccg cggcgcatgc ggccggcacg agcgcgctgc tgccgatgct
     3061 cacacttggc ataccagggt cacctaccgc cgccgtgctg ctcggcgcgc tcctcatgtg
     3121 ggggcttcag cccggcccga tgctcttcgt cgagcagaag gatttcgtct ggggcctgat
     3181 cgcctcgatg tatctcggta atctcgccgg cctgatcgtg gtgctcacgt gcgtgccctt
     3241 gttcgcggcg attctgcgca tccccttctc gatcatcgcg ccgatgatcc tggtgatctg
     3301 cgtggtcggc gcctacaccg tcaagaacag catgttcgac gtctgggtga tgctgctgtt
     3361 cggtgtcgca ggctacggct tcaacaagct cggctatccc ctcgccccgc tcgtcctcgc
     3421 catcatcctc ggcgacaagg cggaggaggc attccggcag gccctgctcg gcagccaggg
     3481 cagcctgctc gtcttctggt acgacgctaa taccggcatc aactggctcg ccggctcgat
     3541 catgacgctc ggcttggcgc tcatcgccat gccgatgttc gagtgggctc gaggcctatc
     3601 gcgtcgccca ccgccggctg ccgcggcagt cgcgccggca gccggcacgg gcccgagcag
     3661 cggcagcgcg tccagcgccg ccgcgccgag cagccctgcg gtcgccagag cgagtgcagc
     3721 cgtgcaacct gtggcacca
//
LOCUS       DAORNH010000375         3734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18534_length_3734_cov_2.787986, whole genome shotgun sequence.
ACCESSION   DAORNH010000375 DAORNH010000000
VERSION     DAORNH010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18534_length_3734_cov_2.787986"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK264_23635"
     CDS             <1..314
                     /locus_tag="PK264_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78894.1"
                     /translation="WLGVESVFASQPRQVLENICAKGCIIRLNDNDNEDDEYVLDGTE
                     IVAIQDGTLYLDGPDNPSQPNVGTEGPKGADPKAPPKAVDPKAGDPKPAAPKADEPKK
                     Q"
     gene            complement(377..745)
                     /locus_tag="PK264_23640"
     CDS             complement(377..745)
                     /locus_tag="PK264_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRD78895.1"
                     /translation="MKHCLIVDDSSVVRKVARQILEGMRFEVAEAENGQQALELCQSQ
                     RPDAIIVDWHMPVMGGLDFLNAYRHMHGSQRPFIIYLTTENDPVDISRAFAAGADDYV
                     LKPFTRENIEAKFIAAGMAS"
     gene            1078..2004
                     /locus_tag="PK264_23645"
     CDS             1078..2004
                     /locus_tag="PK264_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRD78896.1"
                     /translation="MAGSQPCLDGKVVVVTGAGRGIGREMAILAAKEGAKVVVNDLGS
                     SVDGEGASSQQPADEVVETIRKAGGKAVANFESVSEPKSAENIIKTAIDAFGRVDGVI
                     NNAGILRDRIFHRMSVVDFDQVIKVHLYGAFYVSRAAANHFKDQNGGAFVHFTSTSGL
                     VGNFGQANYAAAKLGIVGLSKSIALDMQRYNVRSNCISPFAWSRMIGTIPTETEAQKA
                     RVERMKRMTPDKIAPLAVFLLSDLAKEVSGQIFGSRMHEIFLFSQNRPVRSVQREAGW
                     TPQTIAEHALPAMKASFTPLDRSADVFSWDPV"
     gene            2351..3466
                     /locus_tag="PK264_23650"
     CDS             2351..3466
                     /locus_tag="PK264_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78897.1"
                     /translation="MEYFLNLFSPETWSAFRAAGRTISGFSRHQKTHAQRSLQPGNIF
                     LCYLVGLGRWCGALEIESEAFVDEAPIFKPVSDPFVVRFRVTPLVVLAPEHALPMREP
                     SLWNRLAWTKDKSPGAVGWGANFQRSLRRMPEEDGAFLLDLLTKQAQTLKPYELSGKD
                     RRALNRATVRTATGELPVEIPDGDDETEGLAEDRVKELEVRQSHKIQAMIAEIGSKMG
                     FKIWLPKSDRERVKTATSYDLNATIIEILPMNYNDATIRTIEQIDVIWLKGRSIARAF
                     EIEHTTAIYSGLLRMADLVALQPDIDIPLHIVAPESRQSAVLDQIKRPVFSLLETGPL
                     SDRCSLLTYDDIEEIAAKPDLAHMRDSILDDYQVFAH"
BASE COUNT          756 a         1141 c         1152 g          685 t
ORIGIN      
        1 tctggctggg cgtcgagagc gtcttcgcca gccagccgcg ccaggtgctc gagaacatct
       61 gcgcgaaagg ctgcatcatc cgtctcaacg acaacgacaa cgaggacgac gagtacgtgc
      121 tcgacgggac ggagatcgtc gcgatccagg acggcacgct ctatctcgac gggcccgata
      181 atccctcgca gcccaatgtc ggcaccgaag gcccgaaggg cgccgatccg aaggcgccgc
      241 caaaggctgt cgaccccaag gccggcgacc cgaagcccgc ggcgcccaag gccgacgagc
      301 cgaagaagca ataagacgcg caggagcact cgctgcgcgg agccctgctc ggcagggccg
      361 tttcaactca tcgagctcag ctcgccatgc cggcggcgat gaacttcgcc tcgatgttct
      421 cgcgcgtgaa gggcttcagc acatagtcgt cggcgccggc ggcgaacgcg cgcgagatgt
      481 cgacgggatc gttctccgtc gtcaggtaga tgatgaaagg ccgctgcgat ccgtgcatgt
      541 gacggtaggc attgagaaaa tctaggccgc ccatcaccgg catgtgccaa tcgacgatga
      601 tcgcgtcggg ccgctgggac tggcagagct ccaacgcctg ctggccgttc tccgcctcgg
      661 ccacctcaaa acgcattcct tcgaggatct gtcgcgccac cttgcggacg acactcgagt
      721 cgtcgacgat cagacagtgc ttcatggcaa gcccctgcac gtttccgagg cagcgcgaga
      781 attcgtgcac gccccttcct gcacagtcgg caagattggt taattttctg cgaaccgagc
      841 gggctccggc gcgcctttca tgtcggcgag ctgaggccgg ccaaccgagg tctcgtctcc
      901 ggagcagccg gcagttggcc ggtatcgttc ctttgatgcg cgcggccgta ctgcaaagct
      961 tgcggcctct gcggcgccac gaatgctcaa accgctcatg gctccgtatg acagagcgtt
     1021 tgacgcccgt cggcgctgca tgcgagcttc gggtgatcaa cacaaaggag taaactcatg
     1081 gcgggcagtc agccgtgcct cgatggcaag gtcgtggtgg tgacgggggc agggcgcggc
     1141 atcggacgcg agatggcgat cctcgccgcc aaggagggcg cgaaggtcgt cgtcaacgat
     1201 ctcggctctt ccgtcgacgg cgaaggcgcg tcgagccagc agcccgccga cgaggtggtc
     1261 gagacgatcc gcaaggccgg cggcaaggcg gtcgccaatt tcgagagcgt ctcggaaccg
     1321 aagagtgcgg aaaacatcat caagactgcg atcgacgcct tcggccgcgt cgacggcgtg
     1381 atcaacaacg ccggcatcct gcgcgaccgc atcttccacc gcatgagtgt cgtggacttc
     1441 gatcaggtca tcaaggtgca cctctacggc gccttctacg tctcgcgcgc cgccgccaac
     1501 cactttaagg atcagaacgg gggtgccttc gtgcacttca cgtcgacctc gggcctcgtc
     1561 ggcaacttcg gccaggcgaa ctatgcggcg gccaagctcg gtatcgtcgg cctctccaag
     1621 tcgatcgctc tcgacatgca gcgctacaac gtgcgctcca actgcatctc gccgttcgcc
     1681 tggagccgca tgatcggcac catcccgacc gagacagagg cgcagaaggc gcgcgtcgag
     1741 cgcatgaagc gcatgacgcc cgacaagatc gcgccgctcg ccgtgttcct gttgtctgat
     1801 ctcgccaagg aggtttcggg gcagatcttc ggcagccgca tgcacgagat cttcctgttc
     1861 agccagaacc ggcccgtccg ctccgtgcag cgcgaagcgg gctggacgcc gcagaccatc
     1921 gccgagcacg cgctccccgc gatgaaagcc tccttcacgc ccctcgaccg ctcggccgac
     1981 gtgttcagct gggatccggt gtgagggaag gaagctcgag gggcgccgcc gtcgtgctac
     2041 aagcatgctt tcagcacgac gagtggcgac cgggggccag ggtgagtatc aagatcgtcg
     2101 aaggggcgtg atggcggatt gggggcaggt gcggactcac taggcgcagc aagacgtgcg
     2161 tcgcgcaaaa ctggatcatc atgcaagccg accttgaacg gatagcgatt ggcatcgatt
     2221 ttggcaccct tgagcacgat cgctgaccgg atcatcgcgt cgctctgttg acgttgagtc
     2281 attaggctcg ctgaacgtca ggaacttctg catatgaggt tgaccagatc aggccggatc
     2341 gaccagacag atggaatact tcctcaacct cttctcgccc gaaacgtggt cagcgttccg
     2401 agccgcaggc agaaccatca gcgggttttc tcggcaccag aagacgcatg cgcagcggtc
     2461 gcttcaacca ggtaacattt ttctctgcta tctcgtgggc ctcggcagat ggtgtggagc
     2521 acttgagatc gagtcagagg cgttcgtcga cgaggcgcca attttcaagc cagtgtccga
     2581 ccccttcgta gttcggtttc gcgtcacgcc tctcgtagtc ctcgcccccg agcatgcgct
     2641 gccaatgcgc gaaccgagcc tgtggaaccg gttggcttgg acaaaggata aaagtcctgg
     2701 ggctgtcggg tggggagcaa acttccagcg ctcgctccgt cgtatgcccg aagaggatgg
     2761 agcttttctc ctcgaccttc tgaccaagca agcccaaacg ctgaagccat atgagttaag
     2821 tggcaaagat cgcagggcac tgaaccgagc aaccgtgcgg accgcgacgg gagaactgcc
     2881 tgtagaaatt ccagatgggg acgatgagac ggaggggctg gctgaagaca gggtgaaaga
     2941 actcgaggtt cgtcagtctc acaaaatcca ggcgatgatc gccgagatcg gcagtaagat
     3001 gggattcaag atttggctac caaaatctga ccgcgagcgc gtgaagacag ccacgtcgta
     3061 tgacctgaat gccaccataa tagaaattct gccaatgaac tacaacgatg ccacgatccg
     3121 tacgattgag cagatcgacg tgatctggct caaagggcgt tcgatcgctc gagcattcga
     3181 gatcgagcac acaaccgcca tttattctgg cctcctgcgc atggcagact tggtggcact
     3241 gcaacccgat attgatattc cacttcacat cgtagcgccc gagtcacgac aatcggccgt
     3301 actggatcag ataaagaggc ctgttttcag cctattggag accgggcctc tttcggatcg
     3361 ctgtagtttg ctaacctacg atgatattga ggaaattgcc gccaagcccg acttagcgca
     3421 tatgcgcgat agcatcttgg acgactatca ggtgttcgct cattgatgcg aatacccaac
     3481 agcattcaga gcgccttagg gtctgctgtc tgctcaacgt acggtttcca aaggccaagg
     3541 cctttggcgg ggctatcggg gcggagcccc gatagctccg ctcacgctgc tcacctccgc
     3601 cggatgatct cgctccagcg gcgcgtcgtg tgacactcgg cacagttcgg gcggcggccg
     3661 cggtgcgggt cgtcggcctt gtggcaggcg atgcaggcgc gcgtggaggc cgccgtcgca
     3721 gccggactgc cgct
//
LOCUS       DAORNH010000376         3733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18546_length_3733_cov_2.620446, whole genome shotgun sequence.
ACCESSION   DAORNH010000376 DAORNH010000000
VERSION     DAORNH010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18546_length_3733_cov_2.620446"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    214..234
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <235..1093
                     /locus_tag="PK264_23655"
     CDS             <235..1093
                     /locus_tag="PK264_23655"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HRD78898.1"
                     /translation="SQDGRTIVSGSWDNSLRLWEASTGRLIRTLEGHGNSVLSVAISP
                     DGRTIASGSNDDTVKIWEASTGRLLRTFGGHKSSVLSIAMSPDGKTVVSGSADKTVTL
                     IDAATGRLGRTFEGNKNWVSSVAVSPDGKTIVSGGWDKKVRLSEASTGRLIRTFDGHA
                     NLVASVAVSPDGRTIVSGSDDRTVKLWATATGRLIRTLEGHAAGVSSVAVSSDGRTIV
                     SGSTDRTVRLWESSTGSLIGMLEGHGHAVHSIAVMPDGRQLVSAGCEAFDRDKGLCTR
                     GEIKIWSMP"
     gene            complement(1603..>3733)
                     /gene="infB"
                     /locus_tag="PK264_23660"
     CDS             complement(1603..>3733)
                     /gene="infB"
                     /locus_tag="PK264_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRD78899.1"
                     /translation="SRRPPNITIKVDAPPVPPPSREEKRGDAAVRKVEPAKRPPTHAR
                     KPVDDEEEESPARKVDPRGGKKVVKVERPQPKSSEERRERVKLTISNFDREAQERSLA
                     SLKRKREREKLRAMGVQQPREKIVREVIIPEVITIQELSNRMAERGVDLIKFLMKQGA
                     MHKINDVIDADTAELIATEFGHKPRRVSEADVEEGFIGAQDDHGNLKSRAPVVTIMGH
                     VDHGKTSLLDAIRKTSVASGEAGGITQHIGAYQVTMADGQKITFIDTPGHEAFTAMRA
                     RGAKVTDIVILVVAADDGVMPQTVEAINHAKAANVPMIVAINKVDKHDADPTRVATEL
                     LQHEVVVESMSGETLDVPVSAIKGTNLDKLLEAILLQSEILDLKANPERSAEGFVIEA
                     KLERGRGPVGTLLVQRGTLRVGDIVVAGHAWGRVRALIDDRNKTLDEAGPSVPVEVLG
                     FDTAPEAGDQFAVVENEARARELTEYRSRKRRDTLGPASSVRTLEQMMQQIKSADRKE
                     FQLLVKGDVQGSVEAINGALKKLGTDEVAARIVHSGVGGITESDVALAATSGSVIVGF
                     NVRANAQAKQAADNQGVEIRYYNIIYDLVDDVKKAMSGLLAPTKRELFLGNAEILEVF
                     NISKVGKVAGCRVTEGKVERGARVRLLRDNVVIHEGTLAILKRFKDDVREVPVGQECG
                     MSFANYQDIRKGDVIECFQVETVVRSL"
BASE COUNT          581 a         1238 c         1225 g          668 t
ORIGIN      
        1 ccgcgccggc ggcgccaccc gtcgcaaccg ctccaatgcc gcccgccacc ggccccgcca
       61 tcgtcccgcc cggcacgaag ccggggccgt tcgaggaccg catgtcggcg ctgctcgaca
      121 agatgccggc caagcctccg cagcccgctg caccagtcgc cctcgccccg cccggaacca
      181 agcccgggac gctagtgaga tcgtgggaag cgcnnnnnnn nnnnnnnnnn nnnnatcgca
      241 ggacggccgt acaatcgtca gtggcagctg ggacaactcg cttaggctct gggaagcgtc
      301 tacggggcga ctgatccgca cgctcgaggg acacggcaac tcggtgttgt ccgtcgccat
      361 atcgccggac ggccggacga tcgcgagtgg cagcaatgac gatacggtga agatctggga
      421 agcatcgaca ggccggttgc tccgtacgtt cggagggcac aaaagttcgg tattgtccat
      481 cgccatgtcg ccggacggca agacggtcgt cagcgggagc gcagacaaga ccgtaacgct
      541 catcgatgca gcaacgggca ggttgggccg gacgtttgag ggcaacaaga actgggtcag
      601 ctccgttgcc gtgtcgccgg atggcaagac gatcgtcagc gggggctggg acaaaaaggt
      661 gaggctttcg gaagcttcga ccggccggct gatccgcaca ttcgacgggc atgcaaacct
      721 ggtcgcatct gtggcggtgt ctccggacgg ccggacgatc gtcagcggca gtgacgacag
      781 gacggtgaaa ctctgggcga cagcgacggg gcggttgatc cgcacgctcg aagggcatgc
      841 tgctggggtg tcctccgtcg ctgtgtcatc agacggtcga acaatcgtca gtgggagtac
      901 cgacagaact gtaaggctat gggagtcatc gactgggagc ttgatcggca tgctcgaagg
      961 ccatggccac gcggtgcaca gtatcgcggt gatgcccgac gggcgccaac tcgtgagcgc
     1021 cggctgtgaa gccttcgacc gcgacaaagg actgtgcacc cgcggcgaga tcaagatttg
     1081 gtcgatgccg tagaactgag ccctcgcgaa gcccaacaat ttcggtctca ctcggggccg
     1141 gtgggcaacg ctgcgcgcag cgccaaccta cgtccgagtt gaaccgcccg cgccgctcgc
     1201 cccaccatcg tcatcctcgc caaggcgagg acccaggtca ccgcgcgtgg gaccgtaacg
     1261 gccactccga cgtgccctgg gtgccggcct tcgccggcat gacggagtgg gtggggcatc
     1321 gtggcggctc tatcgtaacc gcttccccac actcgtcatc ctcgcgaagg cgaggacctt
     1381 ggccacggtg tgggccataa ggctccgctc gccaaggcgt tgcgcccgcg cccggtgcca
     1441 tggaacggcc actcctccgg gatgctcccc cgacattgct tcggctggct tgcatgagcc
     1501 atccgggaca ctggcctcgc caatcgaccc ggctggcatg attgaggccg ccggattgca
     1561 ccggcggcct ctcgatgttt cttcgctgca gcgcccgacg gatcagagcg agcggaccac
     1621 ggtctcgacc tggaagcact cgatcacgtc gcccttgcgg atgtcctggt agttggcgaa
     1681 cgacatgccg cattcctggc cgacgggcac ctcgcggaca tcgtccttga agcgcttcag
     1741 gatggcgagc gtgccctcgt gaatgacgac gttgtcgcgc agcaggcgga cgcgcgcccc
     1801 gcgctccacc ttgccttccg tcacgcggca gccggcgacc ttgccgacct tcgagatgtt
     1861 gaacacctcg aggatctcgg cgttgccgag gaacagctcg cgcttggtcg gggcgagcag
     1921 gcccgacatc gcctttttca cgtcgtccac gagatcgtag atgatgttgt agtagcggat
     1981 ctcgacgccc tgattgtctg ccgcctgctt cgcctgcgca ttggcgcgca cgttgaagcc
     2041 gacgatcacc gagcccgatg tcgcggcgag cgccacatcc gattccgtga tcccgccgac
     2101 gcccgaatgg acgatgcggg ccgcgacctc gtcggtgccg agcttcttca aggcgccatt
     2161 gatcgcctcg accgagccct gcacgtcgcc cttcacgagg agctggaact ccttgcgatc
     2221 ggcgctcttg atctgctgca tcatctgctc gagcgtgcgc accgagctcg cggggccgag
     2281 cgtgtcgcgg cgcttgcgcg agcggtactc ggtgagctcg cgggcgcggg cctcgttctc
     2341 gacgacggcg aactggtcgc cggcctctgg tgccgtatcg aagccgagca cctcgacagg
     2401 aacggagggg cccgcctcat cgagcgtctt gttgcggtcg tcaatgagtg cacgcacgcg
     2461 cccccaggca tggccggcga cgacgatgtc gccgacacgg agcgtgccgc gctgcacgag
     2521 cagtgtgccg accggaccgc gaccgcgctc gagcttcgcc tcgatcacga agccttccgc
     2581 cgagcgttcc gggttcgcct tgaggtcgag gatctccgac tggagcagga tcgcctcgag
     2641 cagcttgtcg aggttggtgc ctttgatcgc cgagaccggg acatcgagcg tctcgcccga
     2701 catgctctcg acgaccacct cgtgctggag cagctcggtc gcgacgcggg tcgggtcggc
     2761 gtcgtgcttg tcgactttgt tgatggcgac gatcatcggc acattggctg ccttcgcgtg
     2821 attgatcgcc tcgacggtct gcggcatcac gccgtcgtcc gccgcaacga ccaggatcac
     2881 gatgtccgtc accttggcgc cgcgggcgcg catggcggtg aaggcctcgt ggccaggagt
     2941 gtcgatgaag gtaatcttct gcccgtccgc catcgtcacc tggtaggcgc cgatgtgctg
     3001 cgtgatgccg cccgcctcgc cgctggcgac gctcgtcttg cggattgcgt cgagcagcga
     3061 cgtcttgccg tggtcgacgt ggcccatgat cgtgacgacg ggcgcgcgcg acttgagatt
     3121 gccgtggtcg tcctgggcgc cgatgaagcc ttcctcgacg tcggcctccg agacgcgccg
     3181 cggcttatgg ccgaactccg tcgcgatgag ctcggccgta tcggcgtcga tcacatcgtt
     3241 gatcttgtgc atcgcgccct gcttcatcag gaacttgatg aggtcgacgc cgcgctcggc
     3301 catgcggttc gacagttcct ggatggtgat cacctcgggg atgatcacct cgcgcacgat
     3361 cttctcgcgt ggctgctgca cgcccatggc gcgcagcttc tcgcgctctc gcttcctctt
     3421 cagcgaggcg agcgagcgtt cttgtgcctc gcggtcgaag ttcgagatgg tgagcttgac
     3481 gcgctcgcgg cgctcctccg atgacttcgg ctgcgggcgc tcgaccttca cgaccttctt
     3541 gccgccgcgc ggatccacct tgcgagcggg tgactcctct tcctcatcgt ccaccggctt
     3601 ccgcgcatgc gtcggcgggc gcttcgccgg ctcgaccttg cggacggccg cgtcgccccg
     3661 cttctcctca cgcgacggcg gcggaacggg cggcgcgtcg accttgatcg tgatattcgg
     3721 cggacggcgc gag
//
LOCUS       DAORNH010000377         3671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_18974_length_3671_cov_2.929480, whole genome shotgun sequence.
ACCESSION   DAORNH010000377 DAORNH010000000
VERSION     DAORNH010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18974_length_3671_cov_2.929480"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PK264_23665"
     CDS             complement(<1..723)
                     /locus_tag="PK264_23665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRD78900.1"
                     /translation="MSKNGAESGAAASADLPPREAMEFDVVVVGAGPAALATAIRLKQ
                     LAAAKGSELSVVVIEKGSEVGAHILSGAVIDPIGINRLIPDWKAKGAPIETEVTDDRF
                     IYLGPSGDLRLPNRLMPPLMSNHGNYIVSLGQLCRWLGEQAAELGVDIYPGFAASEVL
                     YDERGSVVGVATGDMGVGRDGQPKDAFTRGMELRGKYTVIAEGARGSLAKQLIAKFAL
                     DKGRQPQKYGIGLKELWEVRPDR"
     gene            complement(843..1778)
                     /locus_tag="PK264_23670"
     CDS             complement(843..1778)
                     /locus_tag="PK264_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRD78901.1"
                     /translation="MRAVNRIILDKAISDVELIPKLAHHLIDSGGKRLRPMLTIAAAK
                     LVGYSGEGHIRLAATVEFMHTATLLHDDVVDDSDMRRGKKAARMIWGNQSAVLVGDFL
                     LGQAFRMMVDVGSLPALKILSNAAAVIAEGEVMQLAAAKNTATTEDQYLAIIDAKTAA
                     LFAAAAEVGPAIAHKPVEVQAALRSYGRNLGIAFQLVDDALDYAGDGARLGKSVGDDF
                     REGKITLPIILAYRRGSQVERDFWARTLTRGEIGDGDLEHAIGLLRRHRAIEATLDRA
                     RHYGTIARDALAVFPDGAPRTALLDAVGFCVSRAH"
     gene            2008..2802
                     /locus_tag="PK264_23675"
     CDS             2008..2802
                     /locus_tag="PK264_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRD78902.1"
                     /translation="MPQPDTIPPDASDDRFLGGRIVLRQPRRGYRAGLDAVLLAAGGA
                     MVDAAGDTGSRRILDLGAGVGGAGLCLASRVAEAHVTLVEREAQLAALARHNVSTNGF
                     DDQVDVIEGDAESRWSALAAAGLQRESYDNVICNPPFYADASGTAPAAHLKAMAHQMA
                     TSGLQLWIRTLATAAAAGGTLTLIHRTEALADVLGAIGDRFGAIGVLPIAPRCGKDAI
                     RILVRGVKGSRAPLRLASPLVLHDAEGRFCPEVDDILRNGAALVWP"
     gene            2857..3210
                     /locus_tag="PK264_23680"
     CDS             2857..3210
                     /locus_tag="PK264_23680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78903.1"
                     /translation="MDLIQHLRAAAEHETSYFRSHLLKEDVARLERAASLARMLPDEA
                     SYLAQATKLGWTADDRRTHELMPALKPFLIAFRRAETGGRTDIGPELLELWRELDTLR
                     MARLVGCLSRVPTPE"
     gene            3253..>3671
                     /locus_tag="PK264_23685"
     CDS             3253..>3671
                     /locus_tag="PK264_23685"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD78904.1"
                     /translation="MSKIILVTGGARGIGAATARLAAARGYDVAINYASRPDAAEAVA
                     RDVSAAGRRAAIFRADVASEAQVKAMFSGIDRELGRIDVLVNNAGILHPMSRFEETPE
                     GQFEAMFAVNVFGVVYATREAIRRMAPRHGGKGGVIV"
BASE COUNT          610 a         1218 c         1250 g          593 t
ORIGIN      
        1 acggtctggc cggacctccc agagctcttt caggccgatg ccgtacttct gcggttgccg
       61 ccccttgtcg agagcgaact tggcgatcag ctgcttggca agcgagccgc gcgctccttc
      121 cgcgatcacg gtgtacttgc cgcgcagctc catgccgcgc gtgaacgcgt ccttcggctg
      181 gccgtccctg ccgacgccca tgtcgcctgt cgcgacaccg acgaccgagc cgcgctcgtc
      241 gtagagcacc tcggaggcgg cgaatcccgg gtagatgtcg acgccaagct cggccgcctg
      301 ctcgccgagc cagcggcaga gctgcccgag gctcacgatg tagttaccgt ggttgctcat
      361 cagcggcggc atgaggcggt tcggcaggcg gaggtcgccg gaggggccga ggtagatgaa
      421 gcggtcgtcc gtcacctcgg tctcgatcgg cgcacctttc gccttccagt cggggatcag
      481 ccggttgatg ccgatcggat cgataacggc gccggataga atgtgcgcac cgacctcgga
      541 gcccttctcg atgacgacga cggagagttc ggagcccttg gcggcagcga gctgtttcag
      601 gcggatagcg gtcgcaaggg cggctggccc ggcgccgaca accacgacat cgaactccat
      661 ggcctcacgg ggtggaagat ccgcgctggc cgccgctcca ctctcagccc cgttcttcga
      721 catctcgacc tctcgctgag ccccgacgcc gagcccgccc gccggccggc ccgctcgatc
      781 accgcgatcg attaggtccg cccgcgcccg ggcgtcaagc cgaagaaacg cggatcaggg
      841 cgtcaatgag cccggctcac acaaaagccg acagcgtcga gcagtgcggt ccgcggcgcg
      901 ccatcgggga aaacggcgag cgcgtcgcgg gcgatcgtcc cgtagtggcg ggcgcggtcg
      961 agagtcgcct cgatcgcccg atgacggcgg aggaggccga tcgcgtgctc gagatcgccg
     1021 tcgccgatct cgccgcgggt gagcgtgcgg gcccagaaat cccgttcaac ctggctgccg
     1081 cggcgatagg cgagaatgat cggcagggtg atcttgccct cgcggaagtc gtctccgacc
     1141 gacttgccga gacgcgcacc gtcgccagca tagtcgaggg cgtcatcgac gagctggaag
     1201 gcgatgccga ggttgcgacc atatgaacgc agcgccgcct gcacctcgac gggcttatgg
     1261 gcgatcgccg gcccgacctc ggcggcggcg gcgaacagcg ccgcggtctt ggcatcgatg
     1321 atggcgaggt actggtcctc ggtcgttgcc gtgttcttgg ctgcggcgag ctgcatcacc
     1381 tcgccttcag cgatcacggc agcggcgttg gaaaggatct tgagggccgg cagcgagccg
     1441 acgtcgacca tcatgcggaa ggcctgtccg agcaggaagt caccgacgag caccgcgctc
     1501 tggttgcccc agatcatgcg ggcggccttc ttgccgcggc gcatatcgct gtcgtccacg
     1561 acgtcgtcgt ggaggagcgt tgccgtgtgc atgaactcga cagtcgccgc gaggcggatg
     1621 tggccctcac ccgagtagcc gaccagcttg gcggcagcga tcgtgagcat cgggcggagc
     1681 cgcttgccgc ccgaatcgat caggtggtgc gcgagctttg gaatgagctc gacgtccgag
     1741 atcgccttgt cgagaatgat gcggttgacc gcacgcatgt cctcagcaac aagcgtcagg
     1801 agcggctcga ggcttgcagc cggctcgcgc gcttcatcga gggggacgac gactcccaaa
     1861 gctggcttcc tgattgatcc gctcatatga cccatcgaac gaacttttct acccggacgg
     1921 ctcgctgccg gatcacgtca gattgccgca tcttggaggt cggcggcaac ccgagccgcg
     1981 gctctcggtt cttggcgggc ggtccagatg ccacagcctg acacgatacc gcccgatgcc
     2041 agcgatgacc gatttcttgg ggggcgaatt gtcctgcgtc aaccgcgccg gggctatcgg
     2101 gcggggctgg acgcggtgct gctcgcggcg gggggcgcta tggtcgatgc ggcgggcgac
     2161 accgggagcc gacggatcct cgacctcggt gcaggcgtcg gcggcgccgg gctttgcctc
     2221 gcatcccggg ttgcagaggc tcacgtgacg ctcgtcgagc gggaggcgca gcttgccgcc
     2281 ctcgcccggc acaatgtgtc gacgaatggt ttcgacgatc aggtagacgt gatcgagggc
     2341 gatgccgaga gccgctggtc cgcactggcc gccgctggtc tccagcggga gagctacgac
     2401 aacgtcatct gcaatccgcc attctacgcc gacgcctctg gcaccgcgcc cgccgcacac
     2461 ctcaaggcaa tggcgcacca gatggccacg tcgggactgc agctctggat ccggacgctt
     2521 gcaaccgcgg cagcggcggg cggaacactg acactcattc atcgaaccga agcgcttgcc
     2581 gacgtgctcg gcgcgatcgg ggaccggttc ggcgccatcg gcgtgctgcc gatcgcaccg
     2641 aggtgcggaa aggacgccat tcgcattctc gtgcgcggcg tgaagggcag ccgggcacca
     2701 ttgcggctcg cctcgccgct cgtcttgcat gacgccgaag ggaggttctg cccggaggtc
     2761 gacgacatcc tgcgcaatgg ggcagcactc gtttggccgt agacgcgaaa ccggcgcatg
     2821 tccgataacg gcgggcgaaa gcacgcggag acgaccatgg atctcatcca gcatttgcgg
     2881 gctgccgcag agcacgagac gagctatttt cgcagccatc tcctgaagga ggatgtcgcg
     2941 cgccttgaac gggcggccag tctcgcccgg atgctgcccg acgaggcctc atatctcgcg
     3001 caggccacca agctcggctg gactgccgac gaccgccgaa cgcacgagct gatgccggcg
     3061 ctgaagccat tcctgattgc gtttcgcagg gccgagaccg gaggccgtac cgacatcgga
     3121 cccgaactgc tggagctttg gcgcgaactc gacacactgc gcatggcgcg gctggtcgga
     3181 tgcctctctc gcgtgcccac acccgagtga cgcagcacgt cttcgcaacc gttccctgaa
     3241 ggagccgccg acatgagcaa gatcatcctg gtgaccggag gagcgcgcgg catcggcgcg
     3301 gcaacggccc gcctcgctgc cgcacgcggc tacgacgtcg cgatcaacta cgcctcgcga
     3361 ccggatgccg ccgaggccgt cgcacgcgac gtgtcagctg ccggccgccg cgccgcgatc
     3421 ttcagggcgg atgtggcaag cgaagcgcaa gtgaaagcca tgttctcagg catcgaccgc
     3481 gagctcggcc gcatcgacgt gcttgtcaac aacgccggca tcctgcaccc gatgtcccgg
     3541 ttcgaggaga cgcctgaggg ccagttcgag gcgatgttcg ccgtcaatgt attcggggtc
     3601 gtctatgcga cgcgcgaggc gatccggcgc atggcgcccc ggcacggcgg caagggtggc
     3661 gtgatcgtca a
//
LOCUS       DAORNH010000378         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_19141_length_3645_cov_2.687187, whole genome shotgun sequence.
ACCESSION   DAORNH010000378 DAORNH010000000
VERSION     DAORNH010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19141_length_3645_cov_2.687187"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="lpxB"
                     /locus_tag="PK264_23690"
     CDS             complement(<1..1057)
                     /gene="lpxB"
                     /locus_tag="PK264_23690"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HRD78905.1"
                     /translation="MAAAKDVHGHRDGDADGLRIVLIAGEHSGDALGAKLMAALSARR
                     PGQLQFDGVGGEAMAREGLSSLFPLSDVAVMGPLAIARRLPRIVRRVYQTVDAAVAAA
                     PHAVVIIDSPDFTHPIARRIRRRLPGVPIIDFVSPSVWAWRPKRAPRMRRYVDHVLAL
                     LPFEPEVHRRLGGPLCSYIGHPLVERMDWIGALDPWSLRERLGLDAGRKTLVVLPGSR
                     RSEIERLAGPFGDALAELIRRGLRPQVLVPVLPHVRDLVAAHAADWPVRPQLIEGEDD
                     KFAAFKLADAALAASGTVTLEVALAGTPMIVAYRVDALAARLRFLVKTPHFALANLVL
                     GRRAFPELMQEDCEPRRL"
     gene            complement(1059..1502)
                     /locus_tag="PK264_23695"
     CDS             complement(1059..1502)
                     /locus_tag="PK264_23695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRD78906.1"
                     /translation="MSGTTCLFCRIGAKAIPAVVVHEDADTLAFLDIEPLRPGHTLII
                     PKLHLPYFDALPEPLAMHMMRLGQNLARALKSVHGVDRVGFFYSGGDIAHVHAHVVPL
                     HEKHDITSRRYIAEDTLTFRPPPRAPREALEAEAVRIRARLAVRT"
     gene            complement(1499..2245)
                     /locus_tag="PK264_23700"
     CDS             complement(1499..2245)
                     /locus_tag="PK264_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HRD78907.1"
                     /translation="MTQRGSSSGTGASPRRGFDRTETWIFDLDNTLYPAECNLFAEVD
                     RRMAEYIQRFLGVPYDYAKHLQKAYYRQFGTTLAGMMQVHNMDPTDFLHYVHEIDLTP
                     VPASPALRAAIERLPGRKLIFTAGSHRHAERVAGKLGILDLFEDIIDIVDSGFLPKEK
                     AESYGRLIASHGIEPGACAMFEDMPHNLEPAHLLGMTTVLVHSSKVDHPVQQRIRSWT
                     SVPDHIHHMTEDLAEFLAGIVPAAAQELQP"
     gene            2694..>3025
                     /gene="infC"
                     /locus_tag="PK264_23705"
     CDS             2694..>3025
                     /gene="infC"
                     /locus_tag="PK264_23705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRD78908.1"
                     /translation="MIDETGQNVGVVSRVDAIAKATAAGLDLVEISPDANPPVCKILD
                     FGKFKFQERKKAAEARKNQKVVEIKEIKLRPNIDDHDYDVKMRSVKRFFEEGDKVKIT
                     LRFRGREMA"
     assembly_gap    3026..3153
                     /estimated_length=128
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          650 a         1083 c         1208 g          576 t
ORIGIN      
        1 cgaggcggcg tggctcgcag tcctcctgca tcagctcggg gaaggcgcgc cgaccgagca
       61 ccagattggc gagggcgaag tgcggcgtct tgacgaggaa gcggaggcgc gctgcaagcg
      121 catcgacgcg ataggcgacg atcatcggcg ttccggcaag cgccacctcg agcgtcaccg
      181 tgccagaggc cgcgagcgcg gcgtcagcga gcttgaaggc ggcaaacttg tcgtcctcgc
      241 cttcgatcag ctgggggcgc acgggccaat cggcggcatg ggccgcgaca agatcgcgga
      301 cgtgcggcag caccggaacg agcacctgcg gacgaagccc gcggcggatg agctcggcaa
      361 gcgcatcgcc gaatggaccg gccagacgct cgatctcgga gcggcggctc cccggcagca
      421 cgacgagggt cttgcggccc gcatcgaggc ccaggcgctc gcgcaggctc cacggatcga
      481 gcgcaccgat ccagtccatg cgctcgacga ggggatggcc gatatagctg cacagcggac
      541 caccgagacg gcggtgcacc tctggctcga agggcagcag cgccagcaca tgatcgacgt
      601 agcggcgcat gcgcggcgcc cgcttcggcc gccatgccca cacgcttggc gagacgaaat
      661 cgatgatggg gacgccgggc aggcgtcgcc ggatgcggcg cgcgatcgga tgcgtgaagt
      721 cggggctgtc gatgatgacg acagcatgcg gagccgcggc gacggccgca tcgacggtct
      781 ggtagacgcg acgcacgatc cgcggcaaac gtcgggcgat ggcgagcgga cccatcacgg
      841 cgacgtcgga cagcgggaac agggaggaga gcccctcgcg cgccatggcc tcgccgccga
      901 cgccgtcgaa ctgcagctga ccgggtcgcc gcgcggatag cgccgccatg agcttcgcgc
      961 ccagcgcatc gcccgagtgc tcgcccgcga tcaagacgat gcgaaggccg tccgcgtctc
     1021 catccctgtg gccgtggacg tccttggctg cggccatgtc aggtccggac cgcaagtcgg
     1081 gccctgatcc gcacggcctc ggcctcgagc gcctcgcgcg gggcgcgggg cggcgggcga
     1141 aaggtcagcg tatcctcggc gatgtagcgt cgcgaagtga tgtcgtgctt ctcgtgcagc
     1201 ggcacgacat gagcatgcac atgggcgatg tcaccgccgc tgtagaagaa gccgacacga
     1261 tcgaccccat ggacggattt caaggccctg gccaggttct ggccgaggcg catcatatgc
     1321 atggcaagcg gctcgggaag cgcatcgaag tagggcagat gcagtttcgg gatgataagc
     1381 gtatgccctg gccgcagcgg ctcgatgtcg aggaaggcga gcgtgtcggc atcctcgtgg
     1441 acgacgacgg ccgggatagc cttcgcacca atccggcaga agaggcacgt tgtcccactc
     1501 atggctgcag ttcctgggca gctgccggca cgatgccggc gaggaattcc gcaaggtcct
     1561 cggtcatgtg atggatgtga tccggcacgc tcgtccatga gcggatgcgc tgctgcaccg
     1621 ggtggtcgac cttgctcgag tggacgagca ccgtcgtcat gccgaggagg tgcgcgggct
     1681 cgagattgtg cggcatgtcc tcgaacatcg cgcaagcgcc cggctcgatc ccgtgcgagg
     1741 cgatcaggcg gccgtagctc tccgccttct ccttcggcag gaaacccgaa tcgacgatgt
     1801 cgatgatgtc ctcgaagagg tcgaggatgc cgagcttgcc agcgacgcgc tctgcatgcc
     1861 ggtgggatcc cgccgtgaag atcaatttcc gccccggcag acgttcaatc gctgcgcgca
     1921 gcgccgggct tgcgggaacg ggtgtcaggt caatctcgtg aacgtagtgc aggaagtcgg
     1981 tcgggtccat gttgtggacc tgcatcatgc cggcaagcgt cgtgccgaac tggcggtagt
     2041 aggccttctg gagatgcttg gcgtagtcgt agggcacccc aagaaaacgc tggatgtact
     2101 cggccatgcg tcggtcgacc tcggcgaata ggttgcattc ggccgggtag agcgtgttgt
     2161 cgagatcgaa gatccaggtc tccgttcgat caaagccgcg ccgcggggac gcgccggtgc
     2221 cgctggatga gccacgctgg gtcatgacat ctcgggagac gtgagtgatc agacctgata
     2281 gcccgatcag gtcggattcg ccacaggcag ctgcgaccga agctgcggcc ggccacgccc
     2341 agcgatgccg ccgatgcgca ttcaagaccc tcccatttcg ttacggacgg gcatactgcg
     2401 cggcaagatc ttgactctgc cgccgacttt accgattttt ccttggcctc ctctccaatc
     2461 ctacctggat tgtcgaggcg ggatcagcgc ggcaacctcg ccgcactggt cgatccggcc
     2521 gcggcacagg ctcatgccag cagtttggca cggggctgcg cggatcggtg acagcagcac
     2581 acgtcagcaa cagcccaagg agacgccaca ttcgccgtcc aatgcgcgca gcgcctgaga
     2641 aggaacagac cggcccgaag atcaatgatg cgatccgggc gagagaagtt cgattgatcg
     2701 acgagaccgg ccagaatgtc ggcgtcgtca gtcgtgtcga tgcgatcgcc aaggcgaccg
     2761 cggcggggct cgacctcgtc gagatttcgc ccgacgccaa tccacccgtt tgcaagatac
     2821 tcgatttcgg caagttcaag ttccaggagc ggaagaaggc cgccgaggcc cgcaagaacc
     2881 agaaggtcgt cgagatcaag gagatcaagc tccgaccgaa catcgacgac cacgactacg
     2941 acgtgaagat gcgctccgtg aagcgcttct tcgaagaggg tgacaaggtc aagatcacgc
     3001 tgcgcttccg cgggcgcgag atggcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtcctg gcgcccaagt agcgcagacg
     3181 attcgcgatc tgcgtcggcg gcctgctgat tggcgtccag caggccggcc agacagagat
     3241 tgggcttgtc gaggcgggcg cgtaccgcgg cgcggcgaga tcgttggttg tcaatatttg
     3301 agaacaactc tcattttgtg attcggtcga aattctcgac aacttccacg aagtaggcga
     3361 ttcctttccc tgctgcgacg attcgcagcg tcctgccgtg gttgtgccag cagtctcatc
     3421 gctggtgcgg ccaggtcgag acaacgggag cccaagggcg aagggaaggg agtgcagcat
     3481 ggatccagca gcacttgtcg tcatgctggt cgtcgtctgg tcaggcagct cctttgctca
     3541 gacggggtgg ctgccggcgg ggtatgggct gctgctcacg accctcgtgt acagcgtggc
     3601 actcttctgg tcgatcatga cttcgcgcga gacggtcgcg ccctc
//
LOCUS       DAORNH010000379         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_19259_length_3627_cov_2.205207, whole genome shotgun sequence.
ACCESSION   DAORNH010000379 DAORNH010000000
VERSION     DAORNH010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19259_length_3627_cov_2.205207"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>290)
                     /locus_tag="PK264_23710"
     CDS             complement(<1..>290)
                     /locus_tag="PK264_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRD78909.1"
                     /translation="VERGHLYIAQPPLYKVMRGKSETYLKDERALEDYLIRAGLNDCV
                     LKLATGEGRTGNDLREVIEQARGIASVLDGLHTRYSRAVVTQAAIAGALVPT"
     assembly_gap    291..315
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<316..1907)
                     /locus_tag="PK264_23715"
     CDS             complement(<316..1907)
                     /locus_tag="PK264_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HRD78910.1"
                     /translation="MSAEPQPKADASSYDESQIRILKGLEAVRVRPGMYIGDVHDGTG
                     LHHMVYEIVDNAIDEALAGYCDHVQVSLNPDGSVTVEDNGRGIPVGVKRDDEAKRTAV
                     EIVFTELHAGGKFDNSKEQENAYKVSGGLHGVGAAVVNALSTTFEATVWRDGKEHVIR
                     FADGGATQGPLKITGDAKGRRGTRVTFLPSPQTFTKTDFDYATLEHRLRELAFLNSGV
                     KITFSDRRHADVKTEVMHYEGGLAAYVAWLDRSKKPMLGEPILIRGEQNRIAVEVALW
                     WHDAYSENTLCFTNNIPQRDGGTHLQGFRGALTRSIGKYADDQSKKEKISLTGDDARE
                     GLTCIVSVKVPEPSFSSQTKDKLVSSEVRPVVESIVNDQLGAWFEEHPKETKLIVGKI
                     VEAAQAREAARKARELTRRKGALDMANLPGKLAECQDRNAANTEMFIVEGDSAGGSAK
                     QARNREFQAVLPIRGKILNVERVQRHKMLSSQEIGTLITALGTGIGLDEEGGFKLDKL
                     RYHKIIIMTDADVDGAHIRTLLL"
     gene            complement(2160..3086)
                     /locus_tag="PK264_23720"
     CDS             complement(2160..3086)
                     /locus_tag="PK264_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78911.1"
                     /translation="MPTHTVVVPQLNLRSAPDPTKKNVIATLAQGTPVDKLANSGVAG
                     WFEIAVTIAGTQLRGHVNSRFLGPVSTAFPSASVTAGRLPAADMGSKATEKRSVTGTR
                     THSIKESGKPGRPSTHGSGKAAGILRIIDWMDVGNDAHLRWQPGGGKTFCNIYVYDIA
                     DTAGCYVPRVWWNARAIADLKAGKTVEAKINATVDEVRANYIFKWLEQHGADFGWKRV
                     FDPDVLQTEVNAGRLGIICAQRTDMERPGHIQIIAPEHGEQKAKRSAAGKVTQPLQSN
                     AGSRNFTYGHLGTTWWQGVTFRQFGFWTSETA"
     assembly_gap    3351..3371
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3372..>3627
                     /locus_tag="PK264_23725"
     CDS             <3372..>3627
                     /locus_tag="PK264_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78912.1"
                     /translation="VEEEEGRIAAAPFAKELGLGEKDADKERLLLAGRTRACRHLLWA
                     MLDDEVGAVRAFERASGRAVSVATGRERCSVTVLDLDGGTC"
BASE COUNT          532 a         1140 c         1240 g          669 t
ORIGIN      
        1 aggtcggcac gagggcgccg gcgatcgccg cctgggtgac gacggcgcgg gaatagcggg
       61 tgtgcagccc gtcgagcacc gaggcgatgc cgcgcgcctg ctcgatcacc tcgcggaggt
      121 cgttgcccgt ccgcccctcg cccgtggcga gcttcaggac gcagtcgttg agaccggcgc
      181 ggatcaggta gtcctcgagc gcgcgctcgt ccttcaggta ggtctcggac ttgccgcgca
      241 tcaccttgta gagcggcggc tgcgcgatgt agaggtgccc cctctccacg nnnnnnnnnn
      301 nnnnnnnnnn nnnnnagcag cagcgtgcgg atgtgggcgc cgtcaacgtc ggcgtccgtc
      361 atgatgatga tcttgtggta gcggagcttg tcgagcttga agccgccctc ctcgtcgagt
      421 ccgattccgg tgccgagcgc cgtgatgagc gtgccgatct cctgactcga cagcattttg
      481 tggcgctgga cgcgctcgac gttcaggatc ttgccgcgga tcggaagcac ggcctggaac
      541 tcgcggttgc gggcctgctt ggccgagccg ccggcgctgt cgccctcgac gatgaacatc
      601 tcggtgttgg cggcgttgcg gtcctggcac tcggcaagct tgcccggcag gtttgccatg
      661 tcgagggcac ccttgcggcg cgtcagctcg cgcgccttgc gggcggcctc acgcgcctgc
      721 gcggcctcga cgattttgcc gacgatgagc ttcgtctctt tcgggtgctc ctcgaaccag
      781 gcgccgagct gatcgttgac gatgctttcg acgacagggc gcacctcgga ggagacaagc
      841 ttgtccttgg tctggctcga gaagctcggc tccggcacct tcaccgagac gatgcaggtg
      901 aggccctcgc gggcgtcgtc gccggtgagc gatatcttct ccttcttcga ctgatcgtcg
      961 gcgtacttgc cgatgctgcg cgtgagcgcg ccgcggaagc cctgaaggtg cgtaccgccg
     1021 tcgcgctggg ggatgttgtt ggtgaagcag agcgtgtttt ccgagtaggc gtcgtgccac
     1081 cagagcgcca cctcgacggc gatgcggttc tgctcgccgc gaatgaggat cggctcgccg
     1141 agcatcggct tcttcgagcg gtcgagccag gcgacatagg ccgcgaggcc gccctcgtag
     1201 tgcatcacct cggtcttcac gtcggcgtga cgccggtcgg aaaaggtgat cttgacgccc
     1261 gagttcagga aggcgagctc gcgcaggcgg tgctcgagcg tcgcatagtc gaaatcggtc
     1321 ttggtgaaag tctgcgggct cggcaggaag gtgacgcggg tgccgcgccg tcccttggcg
     1381 tcgcccgtga tctttagcgg gccttgcgtc gcgccgccgt cggcgaagcg gatcacatgc
     1441 tccttgccgt cgcgccagac ggttgcctcg aaggtcgtcg agagcgcgtt gacaaccgcg
     1501 gcgccgacgc cgtgcaggcc gcccgagacc ttgtaggcgt tttcctgctc cttactgttg
     1561 tcgaacttgc cgccggcatg caactccgtg aagacgatct cgactgccgt gcgcttcgcc
     1621 tcgtcgtccc tcttgacgcc gaccggaatg ccgcggccgt tgtcctcgac cgtaacggag
     1681 ccgtccggat tgaggctcac ctggacgtgg tcacagtagc cggcgagcgc ctcgtcgatc
     1741 gcgttgtcga cgatctcgta gaccatgtga tggaggccgg tgccgtcgtg cacatcgccg
     1801 atgtacatgc cgggcctgac gcgcaccgcc tcgaggcctt tcaggatgcg aatctggctt
     1861 tcgtcgtagc tcgacgcatc ggctttcggc tgcggctcgg cgctcatggc agggggttcc
     1921 gtagaggtcg tcatttcacc tgccgcagta tagcaggcgg aaccccgttc cgggtgataa
     1981 atcgtcctcg gggacaagtt cccacaggca attttatcga agcgatatca atatcttgag
     2041 atcaggtcgc gggggatcgg gacgggccct gcacaatcgc cgtggcgagg ccaagctgcg
     2101 aggcacggaa ccggtagcga gacccccgcc gagccccgcc agttccggcc tcgaaaagct
     2161 catgccgtct cgctcgtcca gaagccgaat tgcctgaacg tcaccccctg ccaccaggtc
     2221 gtgccgagat ggccataggt gaaattgcgc gagccggcgt tgctctggag cggctgggtc
     2281 accttgcccg ccgcactacg cttggccttc tgctcaccgt gctcgggcgc gatgatctgg
     2341 atgtgccccg gccgctccat gtcggtccgc tgcgcgcaga tgatgccgag ccgccccgcg
     2401 ttgacctcgg tctgcaggac atcgggatcg aacacgcgct tccagccgaa gtcggcaccg
     2461 tgctgctcga gccacttgaa gatgtagttg gcccgcactt cgtcgacagt cgcgttaatc
     2521 ttggcttcga cggtcttgcc ggccttcagg tccgcgatcg cgcgcgcgtt ccaccacaca
     2581 cgcgggacgt agcagcccgc cgtgtcggcg atgtcgtaga cgtagatgtt gcagaacgtc
     2641 ttgccgccgc ccggctgcca gcgcaggtgc gcgtcgttgc cgacatccat ccagtcgatg
     2701 atgcgtagta tcccggcagc cttgccgctc ccatgcgtcg agggccgccc gggcttgccg
     2761 gattccttga tcgaatgtgt gcgcgtgcct gtcaccgacc gcttctccgt ggccttggac
     2821 cccatgtcgg ctgcgggcag ccgtccggcc gtgacgctcg ccgaagggaa ggcggtcgag
     2881 acgggcccga gaaagcgcga attgacgtgg ccgcgcagct gggtgccggc aatcgtcact
     2941 gcgatctcga accagccggc gacgccgctg ttggcgagtt tgtcgaccgg cgtgccctgg
     3001 gcgagcgttg cgataacgtt cttcttggtg gggtccggcg ccgagcgcag attgagctgg
     3061 ggaacgacaa cggtgtgggt cggcatggct tgctccgaat ggtcatgatg cgaaaaaggt
     3121 agactgcaaa ggcatcgaga acgcggacga tagcgaatcc ggagcgcggc gaacagactg
     3181 atcgcgccgc gtctcgggtc aggcggatgc cggccgaggc gcctcgagcg aaatgcggag
     3241 cgctctgccg gcgagcacct cgaagggctc ggtgtcggtg ccggtcatcc aggcctgcgc
     3301 gccgaggcgg aggaggcggt cgaagagacc gcggcggcgg ctttgatcga nnnnnnnnnn
     3361 nnnnnnnnnn ncgtcgagga ggaggagggg cggatcgccg ccgcgccgtt cgcgaaggag
     3421 ctcggcctgg gcgagaagga cgccgacaag gagcgccttt tgctcgccgg tcgaactcgc
     3481 gcttgccggc atctgctttg ggccatgctt gacgacgagg tcggcgcggt gcgggccttc
     3541 gagcgtgcgt ccggccgcgc ggtctctgtc gcgaccggcc gcgagcgctg cagcgtaacg
     3601 gtcctcgacc tcgacggcgg gacgtgc
//
LOCUS       DAORNH010000380         3623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_19290_length_3623_cov_1.895740, whole genome shotgun sequence.
ACCESSION   DAORNH010000380 DAORNH010000000
VERSION     DAORNH010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19290_length_3623_cov_1.895740"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PK264_23730"
     CDS             <1..462
                     /locus_tag="PK264_23730"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRD78913.1"
                     /translation="AIVRERVRQIMAAGFDGLYLDRIDTWENVSAPGGSRQAMIQFVI
                     EVINYARSLKGDAAIIVQNAEELLTSDSYVAAIDGLAKEDLFHGVHHDGRRNPSGMVS
                     ESLRLLQRLKARGKTIFVIEYLTGSTADGVRAEIRKAGFVPFFSNRSLTGS"
     gene            632..>1426
                     /locus_tag="PK264_23735"
     CDS             632..>1426
                     /locus_tag="PK264_23735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRD78914.1"
                     /translation="MAPTFRVVDTGVREGRLQIAFDQALIELHAAGEIPDTIRFLRFQ
                     PTALIGRHQALSHELHLDRCAAEGIGLVRRITGGGAIYLDEGQVGWEVVLSRKRLAMP
                     TLGDYTRAICEAVAFGLSEAFEIDARFRPRNDIEVGGQKISGTGGFFDGDTLFYQGTV
                     LVDVDPARMMRLLNVPEAKLKKRALDSAEARVTTLKALLGRVPSVAEVHAAVLDGLTR
                     KLGIVCAPGPISEAEERKAKALHAELIGTEAFLREIDDPKAGAETGH"
     assembly_gap    1427..1545
                     /estimated_length=119
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1880..2317
                     /locus_tag="PK264_23740"
     CDS             1880..2317
                     /locus_tag="PK264_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014529708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRD78915.1"
                     /translation="MSTRPRLVPELAVTDIVVSRRFWCQILGFKVLYERPEDGFAYLD
                     LDGAEIMLDQRDGGAPERVGIWDTGPMERPFGRGINFQIRLTDYDATLARVRAAAIAI
                     HFGPEERWYRRGDEEVGVKQFLVLDPDGYIVRLQQIIGRRPRV"
     gene            2387..>3623
                     /locus_tag="PK264_23745"
     CDS             2387..>3623
                     /locus_tag="PK264_23745"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HRD78916.1"
                     /translation="MTPPFTTRPELRGTFGAVSSTHWLATAAGMAMLERGGNAFDAAV
                     ATGFVLQIVEPHLNGPGGEVPIILQRAGRERPEVIAAQGPFPAAAHAARLRSMGLHQM
                     PGTGLLPAVVPGAFDGWMLLLRDYGTLSLREVLAPAIGYAERGFPLVPRIVGTIIPAV
                     DWFKTEWPSSGAVWLVNGRPPHPEKAWRTPAIAETYRRILAEAESAGADRVAQIEAAR
                     RAFYKGFVAEAVDNFFRKELRDSTGRRHAGLLTGDDLARYSATIEAPLAVDYHGVRVH
                     KLGPWTQGIVGLQTLELLKGHDIAALDPVGAPFIHLVVEALKLAFADRETVYGAPEAC
                     ESPVERLLSQDYAKAQRRRIGPSASLTLEPGGFPGAAERMRRVLAQAGVETPVGPGGG
                     EPTFAPLPPEWGDTCHLDCV"
BASE COUNT          571 a         1213 c         1157 g          563 t
ORIGIN      
        1 gcgatcgtgc gcgagcgcgt gcgccagatc atggccgccg gcttcgacgg tctctatctc
       61 gatcgcatcg acacctggga gaacgtgtcg gcgccgggcg gatcgcgcca ggccatgatc
      121 caattcgtga tcgaggtgat caactatgcg cggagcctca agggcgacgc ggcgatcatc
      181 gtccagaacg ccgaggaact cctgaccagc gacagctatg tcgcggcgat cgacggcctc
      241 gcgaaggagg acctcttcca cggcgtccat cacgacggcc ggcgcaaccc atctgggatg
      301 gtgtccgaga gcctccgcct cttgcagcga ttgaaggcac gcggcaagac gatcttcgtc
      361 atcgaatatc tgaccggcag cactgccgac ggcgttcggg ccgagatccg caaggccggg
      421 ttcgtgccgt ttttctcgaa ccgctcgctg acgggcagtt gaggcacccc atgcagcctg
      481 caacacgatc cacacacaag ctgcagcgga tgattgacct ccgctcagca cgcggctcgc
      541 gcgagttgcc atcctcgcgc ggtcaatcga ctttcgaata ttcgaaactg cgaatacagt
      601 ggcgtcttcg cacagcagca aggcgcaacc gatggcaccg accttccgcg tagtcgatac
      661 aggcgtccgc gagggacgcc tgcagattgc cttcgaccag gccttgatcg aactgcacgc
      721 ggccggcgag atcccggaca cgatacgctt cctgcgcttc cagccgacgg cgctgatcgg
      781 ccggcatcag gcgctctcgc acgagttgca tctagatcgt tgcgcagccg agggcatcgg
      841 cctcgtgcgg cgcatcacgg ggggcggcgc aatctacctc gacgaaggcc aagtcggatg
      901 ggaggtcgtg ctgtcgcgga agcgcctcgc catgccgacg ctcggcgact acacgcgcgc
      961 gatctgcgag gccgtcgcgt ttggcctctc cgaagcgttc gagatcgacg cccgcttccg
     1021 cccgcgcaac gacatcgagg tcggcggcca gaagatctcc ggcaccggcg gctttttcga
     1081 cggcgacacg ctgttctatc agggcacagt cctcgtcgac gtggacccgg cccgcatgat
     1141 gcgcctcctc aatgtccccg aggcgaagct caagaagcgc gcgctcgaca gcgcggaggc
     1201 gcgcgtcacg accttgaagg cacttctcgg ccgggtcccc tccgtcgcgg aagtgcatgc
     1261 cgcagtgctc gacggactta cgcggaagct cggcatcgtc tgcgcaccgg gaccaatcag
     1321 cgaggcggaa gagcggaagg cgaaagcgct ccacgccgag ctcatcggca ccgaggcgtt
     1381 cctgcgcgag atcgacgacc cgaaggccgg cgccgagacc ggccatnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncgcgc ctcgtcctcg
     1561 acctcgaagc gagcctgcgc ggcgccctca tcgccgacct gggcccgagc gtcgaccgct
     1621 tctttgccgc aaccaagccc gacatcctga cgatcccccc cgccgagctc aaggccgcaa
     1681 tcgaagaggc ggcgagtagg atcggggtgt aggggcgtcg accctgaggg ggcgaacggg
     1741 gctggtggcg cgagtgagcc aattcgcccg gcaaaggtgt cttccagcgc tttctgttgc
     1801 ctgcggcagc aggcggatgc cattgaagat tcccgcatga tcttgcccag acctcgccga
     1861 tccgcctcga gtgaaagtca tgagcacacg cccgcgcctg gtgcccgaac ttgcggtgac
     1921 cgacattgtt gtgagccgcc gcttctggtg ccaaatcctc ggattcaagg tcctatacga
     1981 gcgtccggag gacggattcg cctatctcga tctcgacggc gctgaaatca tgctcgatca
     2041 gcgcgatggt ggggcgcctg agcgggtcgg catctgggac actggaccga tggagcggcc
     2101 atttgggcgc ggcatcaatt ttcagatcag gctgactgac tacgacgcga cgctcgcacg
     2161 tgtccgtgca gccgccatcg ccatccactt tggaccagag gaacgctggt atcggcgcgg
     2221 cgacgaggag gttggtgtga agcagttcct cgttctcgat cccgacggat acatcgtgcg
     2281 cctgcagcag atcatcgggc gccgaccgcg agtgtgacct accccaccct cggctcccgg
     2341 gcccgactgc gctatggtgg gcgcacctca ctcacgggag cctgccatga cgccaccgtt
     2401 cacgacgcgt ccggagctgc gcggcacgtt cggtgccgtc tcgagcacgc attggctggc
     2461 gacggccgcc ggcatggcga tgctggaaag gggcggcaat gccttcgacg ccgcggtcgc
     2521 gacgggcttc gtcctgcaga tcgtcgagcc gcatctcaat ggcccgggcg gcgaggtgcc
     2581 gatcatcctc cagcgcgcgg ggcgcgagcg gccggaggtg atcgccgcgc agggaccgtt
     2641 ccctgcggcc gcgcatgccg cacggctcag gtccatgggg ctccaccaga tgccgggcac
     2701 ggggctcctg ccggcggtcg tgccgggtgc cttcgacggc tggatgctgc tgctccgtga
     2761 ctacggcacg ctttcactcc gcgaggtgct cgcaccggcc atcggctatg ccgaacgcgg
     2821 gttcccgctg gttccccgca tcgtcggcac gatcattccg gccgtcgact ggttcaagac
     2881 ggagtggccg agctccggtg ccgtctggct cgtgaacggc cggccgccgc atcccgagaa
     2941 ggcctggcgc acgcccgcca tcgccgagac ctaccggcgc atcctcgccg aagccgagag
     3001 cgcgggtgcc gaccgcgtcg ctcagatcga ggccgcgcgg cgcgccttct acaagggctt
     3061 cgtcgccgag gccgtcgaca actttttccg gaaggagctg cgggattcga ccggccgccg
     3121 ccacgcgggg ctcctcacgg gcgacgatct cgcgcgctac tctgcgacca tcgaggcgcc
     3181 gctcgccgtc gactatcatg gcgtccgcgt ccacaagctc ggcccctgga cgcaaggcat
     3241 cgtcgggctg cagacgctcg aactcctgaa gggccacgac atcgccgcgc tcgatccggt
     3301 cggcgcgcct ttcatccatc tcgtcgtcga ggcgctgaag ctcgccttcg ccgatcgcga
     3361 gacagtctac ggcgcacccg aggcctgcga gagccccgtc gagcggctct tgtcccagga
     3421 ctacgcgaag gcgcagcgcc ggcgcatcgg cccgagcgcg tcgctgacgc tcgagcccgg
     3481 aggttttccg ggggcggctg agcgcatgcg ccgcgtgctc gcgcaggccg gcgtcgagac
     3541 gccggtcggg cccggcggcg gtgaaccgac cttcgcgccg ctgccgcccg agtggggcga
     3601 cacctgccac ctcgactgcg tcg
//
LOCUS       DAORNH010000381         3576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_19664_length_3576_cov_1.733882, whole genome shotgun sequence.
ACCESSION   DAORNH010000381 DAORNH010000000
VERSION     DAORNH010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19664_length_3576_cov_1.733882"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>256)
                     /locus_tag="PK264_23750"
     CDS             complement(<1..>256)
                     /locus_tag="PK264_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78917.1"
                     /translation="GEVVTAADAAELERALAILVPARGRIAAGKGPLTLYLTSDTVFR
                     HRATIAGLPREATLHLVLGDETLRLYRLGRGPGQRQFIEV"
     assembly_gap    257..278
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<279..503)
                     /locus_tag="PK264_23755"
     CDS             complement(<279..503)
                     /locus_tag="PK264_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78918.1"
                     /translation="MDLFRQMRMLLASVIPALVMLVLLSSAAAADRPGWLERLIGPAT
                     PIAAGTLDAATHIMKRPPDDKGRVPLAVEGT"
     gene            complement(524..1705)
                     /locus_tag="PK264_23760"
     CDS             complement(524..1705)
                     /locus_tag="PK264_23760"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRD78919.1"
                     /translation="MVPRSAYIVAARRIALGRIGGLHRARRVEDLARPVIDAVLTDAG
                     LSAAKVDGRNQIRIDEVIVGNGAAGANPARLIAMVAGLGSDTPAMTIDRGAASGLDAI
                     ILAKRAIVAGEAECVIAGGAEALSTAPWRVARPRGAHQLPRFLPPTSSQWDPAAPEEG
                     IEAAEAIARRLGIGRAEQDAYVTRALLRIARAASERRLAGEITAVRTAAEEMRDESAS
                     HRRDDALDEAETLIDGGTLTGANTAEPADGAALAVIVSDAVHAHLGKPPALRVTDAVM
                     TAGDPTQLPGAAHAVAHLKQRSAAALWKRLAGIEMSDTSALEAIVTARAAHIDEDLVN
                     ADGGALVRGHAHGAAGAILLARLFTQMIRAPATRRLPVAAAVSGTLDGLGSAALFEAI
                     L"
     gene            complement(1851..2624)
                     /locus_tag="PK264_23765"
     CDS             complement(1851..2624)
                     /locus_tag="PK264_23765"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRD78920.1"
                     /translation="MARVRTSGARKTDRTGTGTLSVFGHQMRFDLADGFPLVTTKKLH
                     MKSIVHELLWFLDGDTNIAYLKANGVSIWNEWADEHGDLGPVYGKQWRSWATPDGRTI
                     DQIGEVMATLRSNPDSRRMIVSAWNPADIPDMALAPCHCLFQFYVAEGRLSCQLYQRS
                     ADIFLGVPFNIASYALLTLMMAKAVGLAPGDFVHTLGDAHLYLNHLDQADEQLRRTPR
                     PLPRLEIATGVASLSDYRFEHFALTGYEPYPHIKAPVAV"
     assembly_gap    2642..2832
                     /estimated_length=191
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2833..>3065)
                     /locus_tag="PK264_23770"
     CDS             complement(<2833..>3065)
                     /locus_tag="PK264_23770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD78921.1"
                     /translation="GDIDWARDDVVAAITERFHARHEELYTYALRGQEPVLVNARVAV
                     IGTLDAPPAEPRLGERPPAGPVRQGRVWLDGWR"
     assembly_gap    3066..3259
                     /estimated_length=194
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3260..>3576)
                     /locus_tag="PK264_23775"
     CDS             complement(<3260..>3576)
                     /locus_tag="PK264_23775"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HRD78922.1"
                     /translation="TRFHGGQLQLDVAAAGTAVDRVATALGLGREAAADGIIRVVDAR
                     MAEGIRLVSVRRGIDPTAFALFSFGGAAGLHASAIARQLSIGRIVVPRIASVLSAWGM
                     LAT"
BASE COUNT          500 a         1056 c         1148 g          465 t
ORIGIN      
        1 ttcacctcga tgaattggcg ctggcccggc ccgcgaccca gccgatagag gcgaagggtc
       61 tcgtcgccga ggacgagatg aagcgtcgcc tcgcgcggca gtccggcgat tgtcgcgcgg
      121 tggcggaaga cggtatcgga tgtcaagtag agggtgagtg ggcccttgcc cgctgcgatt
      181 ctgccgcgcg ccggcacaag gatcgcgagc gcgcgctcga gctcggctgc atcggccgcc
      241 gtgacgacct cgcccgnnnn nnnnnnnnnn nnnnnnnncg tgccttcgac ggcgagcggc
      301 acgcggccct tgtcgtccgg cggccgcttc atgatgtgcg tcgcagcatc gagcgtgccg
      361 gctgcaatcg gggtcgccgg cccgatcaag cgctccagcc atccgggccg gtcggccgcc
      421 gccgcactgc tgaggaggac gagcatgacc agcgccggaa tgaccgacgc cagcaacatg
      481 cgcatctgcc ggaacaagtc catggcggct cggcgattgc cgttcagagg atcgcctcga
      541 atagcgcggc cgagccgagt ccgtccagcg tgccggagac ggccgccgcg acaggcaggc
      601 gtcgcgtggc cggcgcacgg atcatctggg tgaaaagcct tgcgagcagg atcgcaccgg
      661 ccgcgccatg ggcatgtccg cggacaagcg cgccgccgtc cgcgttgacg aggtcctcat
      721 cgatgtgagc cgcacgcgcc gtgacaatcg cctcgagcgc cgacgtgtcg ctcatctcga
      781 tgccggcgag acgcttccag agtgccgcag cgctgcgctg cttcaggtga gcaacagcgt
      841 gagctgctcc ggggagttgc gtgggatcgc ccgccgtcat cacggcatcg gtcactctca
      901 gcgccggagg cttcccgaga tgggcatgca cggcgtcgga gacgatcacc gcgagtgccg
      961 cgccgtcggc cggctcggcc gtgttggcgc cagtcagcgt gccgccgtcg atcagcgtct
     1021 cagcctcgtc gagcgcatcg tcgcgccgat ggcttgcgga ctcgtctcgc atttcctcgg
     1081 cagcggtgcg gaccgcggtg atctcgccgg cgaggcggcg ctcgctcgcg gctcgcgcga
     1141 tcctgagcag ggcgcgggtc acataggcgt cctgctcggc gcgtccgatg ccgagccgcc
     1201 gtgcaatcgc ttcggcagct tcgatcccct cctcgggagc ggcggggtcc cattgcgagg
     1261 acgttggcgg aaggaacctc ggcagctggt gggcgccgcg cgggcgggcg acgcgccatg
     1321 gtgcggtcga cagcgcctcg gcgccgcctg cgatgacgca ctcggcctcg ccggcgacga
     1381 tggcgcgttt cgcaaggatg atcgcgtcga gacccgaggc ggcgccgcga tcgatcgtca
     1441 tcgcgggcgt gtcggacccg agccccgcga ccatcgcgat gagccgcgcc ggattggcgc
     1501 ccgccgcgcc gttgccgacg attacctcgt cgatgcggat ctggttgcgg ccatccactt
     1561 tggcagcgga gagcccggca tcagtcagca ctgcgtcgat caccggccgg gcgagatcct
     1621 cgacccggcg tgcgcgatgc aagccgccaa tgcggccaag cgcgatacgg cgcgcggcga
     1681 cgatgtaagc agaacgtggg accatacgcg gcctctcgca ggcaccccga accggcgtgc
     1741 tcgccccgcg gcgcgccgaa acccgcgctg agagaacacc ctaaacttgg cgaaatggcg
     1801 aaggctccgg ggtgatcgtg cgccaggcgg tggcgctgct gcggcaatcc tcacacagca
     1861 acgggcgcct tgatgtgggg gtacggctca tacccggtga gcgcgaaatg ttcgaaccgg
     1921 taatcggaga gggaggcgac gcccgtggcg atctcgagcc gcggcagcgg ccgtggcgtc
     1981 cgacgcagct gctcgtcggc ctggtcgaga tgattgaggt agaggtgggc gtcgccgagg
     2041 gtgtggacga aatcgcccgg cgcgagaccc acggcctttg ccatcatcag cgtcagcagc
     2101 gcatagctcg cgatgttgaa gggcacgccg aggaagatgt cggccgagcg ctgatagagc
     2161 tggcaggaga ggcgcccttc ggccacgtag aactggaaaa ggcagtggca gggcgccagc
     2221 gccatgtcgg ggatgtcggc cggattccag gccgagacga tcatgcggcg gctgtccgga
     2281 ttcgagcgga gcgtcgccat cacctcgccg atctggtcga tggtgcgccc gtcgggcgtc
     2341 gcccagctcc gccattgctt cccgtagacc gggccgaggt cgccgtgctc gtccgcccat
     2401 tcgttccaga tcgagacgcc gttggccttc aggtaggcga tattggtgtc gccgtcgagg
     2461 aaccagagca actcgtgcac gatcgacttc atgtgcagct tcttggtcgt gacgagtggg
     2521 aagccgtcag cgagatcgaa acgcatctga tgcccgaaca cgctgagcgt gccggtgccg
     2581 gtccgatcgg tcttgcgggc gcccgaggtg cggacgcgtg ccatgaggtc gagatactgc
     2641 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nntcgcgcca gccgtcgagc cagacgcggc cctgccgcac gggaccagcc
     2881 ggcggcctct caccgagccg cggctcagcg ggcggcgcat cgagcgtgcc gatgacggcg
     2941 acgcgcgcgt tgaccagcac cggctcctgg ccgcgcagcg cgtaggtgta cagctcctcg
     3001 tgacgcgcgt ggaagcgctc ggtgatggcg gcgacgacgt cgtcgcgtgc ccaatcgatg
     3061 tcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnc ggtcgcgagc atgccccacg ccgacagcac cgaggcgatg
     3301 cgcggcacga ctatgcggcc gatcgagagc tgccgggcga tcgccgaggc gtggagaccg
     3361 gcggcacccc cgaatgaaaa gagcgcgaac gccgtcggat cgatgccgcg gcggacggag
     3421 acgagacgga taccctcggc catgcgggca tcgaccacac ggatgatgcc gtcggcggca
     3481 gcttcgcgac cgagcccgag cgcggttgcg acgcggtcga cggctgtacc ggcggcggcg
     3541 acatcaagct gaagctggcc gccgtgaaag cgcgtc
//
LOCUS       DAORNH010000382         3529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_19998_length_3529_cov_2.166667, whole genome shotgun sequence.
ACCESSION   DAORNH010000382 DAORNH010000000
VERSION     DAORNH010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19998_length_3529_cov_2.166667"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="PK264_23780"
     CDS             complement(<1..647)
                     /locus_tag="PK264_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HRD78923.1"
                     /translation="MIELAEGDRRMLSGDDGEAAAFAMRLLVRFAEATGAPQLLDIAS
                     AHIDGCLYHGEVSLDFVEHLIKGGGKVRVPTTLNVGSIDLIHPELYLGDVEAASSAKR
                     LMDGHVALGCLPTFTCAPYQSIHRPRLGDQIAWAESNAIVFANSVIGARTNRYGDFID
                     LAAAITGRVPASGLHLSDNRRGRIVFEITAPLGGSFADPGVLAVTMGAIIGERSGG"
     gene            complement(644..>2876)
                     /locus_tag="PK264_23785"
     CDS             complement(644..>2876)
                     /locus_tag="PK264_23785"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRD78924.1"
                     /translation="DAADRVTGRAIYPADFTRPGMVHGHIVRSPHAHARILGIDTTKA
                     AAMPGVLAIVTAADFTALSPGASVPMGDTGQEIWSISLANMARDKAYWIGHPVAAVAA
                     ADRQVAAAAAAAIEVRYEALAAVMSIDASLAPGAPVLHEGHVAKGLDLAPGEKPQPSN
                     VGTRTLITRGDATAALAVAPVRASIDATVDTAHQGYIEPCAVVAEVDAAGMVTVWAST
                     QGQFSMEMQLAAMLALPQSKIRVVPLEIGGGFGGKIATHIEPAAVLLARKCGRPVKIV
                     MSRTEVMHGTGPAAAARIAIEVGADRDGRLTAISGHYRLDAGGIPGTPTTLLMQASAA
                     LYQAHAPALKLEGIDVTTNKPKTEAYRGPGGIQAAFAVEQAMDELSIRLDIDPLELRR
                     RNASVTGSLMPIGTPFPSIGLTTILDRVAAHACWREPLGKGRFPRGRGLALGYWRGTS
                     MTSAAHVTIGADGRPMVTMGSVDISGTRTAMAQVAAEEFQLAIDDVHIQMGDTKSVPY
                     SDPAAGSRVGRTMTAAVAEACRDALAEMRRRAGEKLQCPPTEVRYTKGVFTRGDSGAS
                     ITIAELMRQTTGDGAIIGRGVSTRLPLGVEVGAQVADVEVDTATGQVTVLRFTAFQDV
                     GRALNPPAVEGQIEGSVVQGLGWALTEGFDYDASGRLRNASLLDYRIPTALDVPPIEV
                     VLIETPVPGVPYGVRGVGEIPIVPPAAAVGNAIRRAIGRRLTHMPMTPERVVAALSRL
                     AAE"
     assembly_gap    2877..2886
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3255..3310
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          506 a         1195 c         1232 g          530 t
ORIGIN      
        1 cctcccgagc gctcgccgat gatcgcgccc atggtgaccg ccagtacacc cggatcggca
       61 aaactcccac cgagcggcgc cgtgatctcg aagacgatcc gcccgcgccg gttgtcactg
      121 aggtggagcc cgctcgccgg cacgcgtccg gtgatggcgg cggcaagatc gatgaagtcg
      181 ccatagcggt tcgtgcgcgc gccgatcacc gagttcgcga acacgatggc gttggattcg
      241 gcccaggcga tctggtcgcc gagccgcggc cggtggatcg actgataggg cgcgcacgtg
      301 aacgtcggca ggcagccgag cgcaacgtgg ccgtccatca ggcgctttgc gctgctcgct
      361 gcctcgacat cgccgaggta gagttcggga tggataaggt ctattgaccc gacattgagc
      421 gtggtcggca cgcgcacctt gccgccgccc ttgatcagat gctcgacgaa atcgaggctc
      481 acctcgccat gatagaggca gccgtcgatg tgcgcgctcg cgatgtcgag aagctgcggc
      541 gcgccggtcg cctcggcgaa gcggaccagg aggcgcatgg caaacgccgc cgcctcaccg
      601 tcatcgccgg agagcatccg gcgatcaccc tcagcgagtt cgatcattcc gccgccagac
      661 ggctcagcgc cgcgacaacg cgctcgggcg tcatgggcat gtgggtgagg cgacggccga
      721 tcgcgcggcg gatggcattg ccgacagcgg cagcaggcgg cacgatcggg atctcgccga
      781 caccgcgcac gccgtagggt acacccggca ccggcgtctc gatcagtacg acctcgatcg
      841 gcggcacgtc gagcgcggtc gggatgcggt agtcgaggag gctcgcattg cgcagccgcc
      901 cgctcgcgtc atagtcgaag ccttccgtca gagcccatcc gaggccctgc accacgctcc
      961 cctcgatctg gccctcgacg gccggcggat tgagcgcgcg gccgacgtcc tggaaggcgg
     1021 tgaagcggag caccgtcact tgtcccgtgg cggtgtcgac ctcgacgtcg gcgacctgcg
     1081 ctccgacctc gacgccgagc ggcagcctgg tcgacacacc ccggccgatg atcgcaccat
     1141 cgcccgtcgt ctggcgcatc agctcggcga tggtgatcga ggcgccgctg tcgccgcggg
     1201 tgaatacgcc cttggtatag cgcacctcag taggcggaca ttgcagtttt tccccggcgc
     1261 ggcggcgcat ctcggcgagt gcatcgcggc aggcctcggc gacggcggcg gtcatggtgc
     1321 gcccgacacg gctgccggcg gcagggtcgg agtagggaac ggatttcgtg tcgcccatct
     1381 ggatgtgcac gtcgtcgatc gcgagctgga attcctccgc cgcgacctgc gccatcgccg
     1441 tgcgcgtccc cgagatgtcg actgagccca tcgtcaccat cggccggccg tcggcgccga
     1501 tcgtcacgtg cgcggccgac gtcatcgagg tgccgcgcca gtagccgagg gcgaggccac
     1561 gcccgcgcgg aaaacgaccc ttgccaagcg gctcgcgcca gcaggcatgc gcggcgacgc
     1621 ggtcgaggat ggtcgtgagg ccgatcgagg ggaacggcgt gccgatcggc atcaggctgc
     1681 cggtaaccga cgcatttcgt cggcggagct cgagcgggtc gatgtcgagc ctgatcgaga
     1741 gctcgtccat cgcctgctcg accgcgaacg ctgcctggat gccgcccgga ccgcggtagg
     1801 cctccgtctt cggcttgttg gtggtgacgt cgatgccttc gagcttcagg gccggcgcgt
     1861 gcgcctgata gagcgcggcg gaggcttgca tgaggagcgt cgtcggcgtt cccgggatgc
     1921 cgccggcgtc caggcggtag tgtccactga tcgcggtgag ccgtccgtcg cgatcggcgc
     1981 cgacctcgat tgcgatgcgc gccgccgcgg ccgggcccgt gccgtgcatg acctcggtgc
     2041 gactcatgac gatcttcacc gggcggccgc acttccgcgc gagcagcacc gcggccggct
     2101 cgatgtgggt cgcgatcttg ccgccgaagc cgccgccgat ctcgagcggc acgacgcgga
     2161 tcttggattg cggcagcgcc agcatcgcgg ccagctgcat ctccatcgag aactggccct
     2221 gcgtcgaagc ccacaccgtc accatgccgg cggcgtccac ctcggctacc accgcgcacg
     2281 gctcgatgta gccctgatgc gcggtgtcga ccgtggcatc gatcgaggca cggaccggcg
     2341 ctaccgcgag tgcagcagtg gcgtcgccgc gcgtgatcag tgtgcgcgtg ccgacgtttg
     2401 aaggctgcgg cttctcgcca ggcgcgagat cgaggccctt ggcgacgtgg ccctcgtgaa
     2461 gcacgggcgc gccgggcgcg agcgacgcgt cgatgctcat gaccgcggcg agcgcctcgt
     2521 agcggacttc gattgccgcc gctgctgctg ccgcgacctg gcgatcggcc gctgcgaccg
     2581 ctgcgaccgg gtgaccgatc cagtaggcct tgtcgcgcgc catgttggcg aggctgatcg
     2641 accagatctc ctggcccgtg tcgcccatcg gcacggaggc gcccggagag agcgccgtga
     2701 agtccgcggc cgtcacgatc gcgagcacgc ccggcatcgc ggcagctttc gtggtgtcaa
     2761 tgccgaggat gcgagcatgc gcgtgcgggc tccgcacgat gtggccatgc accatgcccg
     2821 gccgcgtgaa gtcggcgggg tagatggcgc gtcccgtcac ccggtcggcc gcatcgnnnn
     2881 nnnnnncccg cctgccgacg atcgccatcg gcgcgtcggc ctggtcggtc gtatcgttcg
     2941 ctccgagcgt ggtcgccatg tcgctgctcc tggtcctcaa ggccacacga ctggcacgat
     3001 ggcggtcgag tgggcaaggg gatagggagg gcgacggcgc gcggcggcga ccccgcccgc
     3061 cacaatcatc ccaatgaagg gcggcacagc ggtcacttcg gaatggccgc caccgttcct
     3121 acgcccggcg ccctgggtcc tcgccttcgc gaggatgacg agggtggggc gccgacgagc
     3181 acaaccgccc cgggcaagcc gccggctctc gctggcactc ggccgttgaa gcgagcaggg
     3241 cgggcggcac ccacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn gcaggcgatc cgggacccag cgcgacgagg ggcgaagccg ccgtagcttt
     3361 ttacgcggca gtatcgagcg ctccgcgcgc atcttgcgct gggtcccggc tcggcgcgct
     3421 cgcgatgctc acgcttggcc gggatgacag tgatggctgt gcgcgggcgg gccgctaggg
     3481 catcgcccag atcttgaggt cgccgcgggt gcacctgccg ctggccgac
//
LOCUS       DAORNH010000383         3506 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_20183_length_3506_cov_2.752246, whole genome shotgun sequence.
ACCESSION   DAORNH010000383 DAORNH010000000
VERSION     DAORNH010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20183_length_3506_cov_2.752246"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PK264_23790"
     CDS             <1..1187
                     /locus_tag="PK264_23790"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRD78925.1"
                     /translation="IGPSRAQMHAARIPDGQADLVLGCDLVVTASNKVLETIRPDHTT
                     VIYSTYEMMIADFTRKPDFKLPGLELRRAIEDRARKAPVLGLDAHSYALDLFGDSIAS
                     NMFMLGVAFQRGAIPVTATSIEKAIELNGAQIEMNRQAFRFGRLAAHDIAAVDRIIAQ
                     SKQEKAAAKPRSLDDIVAYRVEHLKGYQDAALAERYRSRVSAVATLEREKTPGRTGLA
                     DAVAHGYFKLLAYKDEYEVARLYTDGRFEKALAENFDGELKLEFHLAPPLLSWFSRDK
                     ATGHPRKMSFGKWMLPAFRLLARGKRLRGGMLDVFGYTAERRLERQMIADYERLLDEI
                     GTRLAPETHGIALALARLPEDIKGFGHVKHGNYEAVRRRETALVARLRDPKAPPALKA
                     AE"
     gene            1212..1967
                     /locus_tag="PK264_23795"
     CDS             1212..1967
                     /locus_tag="PK264_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRD78926.1"
                     /translation="MSRAEAKAADMIAGLIREELARRRMTREQLAFKARISLSTLEKA
                     LSGRRPFTLPSVVRLEEALGIALRERQDAEPAAAPMPGTAPDEFGGYARATVGWLEGG
                     YLTLRPSFGSDKAIYAYRTDISWSEERTRLTFRESQRIDADFTQFGDVAVPHQSGHVY
                     LVTNRHGQHRLVMLSRPTITGEMHGLLTTLQAGRGSQLTPVSAPIVLAPLHADGRDAL
                     GRITEGHDAYRRYRRLLALTISDGYAIWRPVGG"
     gene            complement(2025..2534)
                     /locus_tag="PK264_23800"
     CDS             complement(2025..2534)
                     /locus_tag="PK264_23800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78927.1"
                     /translation="MSTLSLRRAIPGVLSIAAAAATLHVAVTAPALAAGPFVSFSGSW
                     SGGGQVRFDGGRTESIRCTAYYTPKDSGAGLTLAIRCGNSSGTKIELRGNLLSKGSSV
                     SGSWEERNFNATGDVTGQATGNRISLSISGGVTGSMSVNLSGSSQAVSITTSGTGFKG
                     LSINLARAS"
     gene            complement(2687..>3506)
                     /locus_tag="PK264_23805"
     CDS             complement(2687..>3506)
                     /locus_tag="PK264_23805"
                     /inference="COORDINATES: protein motif:HMM:NF022515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HRD78928.1"
                     /translation="AGQSQICFQLEQRLIAEGQRGKSSVEVRQKLELEMRQLERQLQT
                     ANASLERSQCYEFWLFNKTLRRTPECVALFNQGEYAKRRLMELDAQRQQILGLGERSM
                     QDELIRELARNNCGSTYTAEAAKRGGTGPFAGWWQDEDAGPSARGGNFGALPYATYRT
                     LCVRLCDGYYFPVSFATLRNHFDKDQDICQSRCAAPVELYYYQNPGGSVEQMVSHRTQ
                     LPYNTLRSAFKYRKEVVTGCSCRKEEYVPSTADRGDRKGDPAATAPVPAKGVPR"
BASE COUNT          582 a         1159 c         1190 g          575 t
ORIGIN      
        1 gcatcggccc gtcgcgcgcg cagatgcatg cggcgcgcat cccggacggg caggctgatc
       61 tcgtgctcgg ctgcgatctc gtcgtcacgg cctcgaacaa ggtgctcgaa acgatccgtc
      121 ccgaccacac cacggtgatc tactcgacct acgagatgat gatcgccgac ttcacccgca
      181 agccagactt caagctgcct ggcctcgagt tacgccgcgc catcgaggac cgcgcgcgga
      241 aggcgccggt actcgggctc gacgcgcaca gctacgccct cgacctcttc ggcgattcga
      301 tcgcctcgaa catgttcatg ctcggcgtcg ctttccagcg cggcgcgatc ccggtgacag
      361 ccacctcgat tgagaaggcg atcgagctca atggcgccca gatcgagatg aaccggcagg
      421 cgttccgctt cgggaggctt gccgctcacg acatcgcggc ggtcgaccgc atcatcgccc
      481 agtcgaagca ggagaaggcc gccgccaagc cgcgctcgct cgatgacatc gtcgcctaca
      541 gggtggagca cctcaaaggc tatcaggatg cggcgctcgc cgagcggtac cgttcgcgcg
      601 tgtcggccgt cgcgactctc gagcgcgaga agaccccggg tcgaacggga ctcgccgatg
      661 ctgtggcgca cggctacttc aagctgctcg cctacaagga cgagtacgag gtggcgcgtc
      721 tctacaccga cggccgcttc gagaaggcgc tcgccgagaa tttcgacggc gagctgaagc
      781 tcgaattcca tcttgctccg ccgctccttt cctggttcag ccgcgacaag gcgacgggtc
      841 atccgcgcaa gatgagcttc ggcaagtgga tgctgccggc cttccgtctg ctcgcgcgag
      901 gcaagcgctt gcgcggcggc atgctggacg tgttcggcta cacggccgag cgccggctcg
      961 agcggcagat gattgccgac tacgagcgac tcctcgacga gatcggaacg cgcctcgcac
     1021 cggagacgca cggcatcgcg ctcgcactcg cgcggctccc cgaggacatc aagggcttcg
     1081 ggcatgtgaa gcatgggaac tacgaggcgg tgcgtcggcg cgagacggcg ctcgtcgctc
     1141 ggctccgcga tccgaaggcg ccgcccgcgc tcaaggccgc cgaataggcg cgggtcaacg
     1201 gggcgaatgg aatgtcgcgc gcggaggcga aggcagcgga catgatcgcg ggtctcatcc
     1261 gtgaggaact ggcgcggcgg cgcatgacgc gtgaacagct cgccttcaag gcgcgcatct
     1321 cactttcgac gctcgagaag gcactctcgg gtcggcggcc gttcacgctg ccgagcgtcg
     1381 tgcgcctcga ggaggcgctc ggcattgcgt tgcgcgagcg ccaggacgca gaaccggctg
     1441 cggcgcccat gccggggacc gcgccggacg agttcggcgg ctatgcgcgt gcgaccgtcg
     1501 gttggctcga aggggggtac ctcacactca ggccctcgtt cggcagcgac aaggccatct
     1561 atgcctatcg gacagacatc tcctggagcg aggagcggac gcggctcacc tttcgcgagt
     1621 cgcagaggat cgacgccgat ttcacgcagt tcggggatgt ggccgtgccc caccagtcgg
     1681 gccacgtcta tctggtgacc aaccgtcacg gccagcatcg tctcgtgatg ctgtcacggc
     1741 cgaccatcac cggggagatg cacgggctgc tgacgacact gcaggcgggg cgcggctcgc
     1801 agctgacccc cgtttcggcg ccgatcgtcc ttgcgccgct ccacgcggac ggcagagacg
     1861 cgctcggccg catcacggag gggcacgatg cctatcgccg ctacaggcgc ctgctcgcgc
     1921 tgacgatcag cgacggctat gcaatctggc gtccggtcgg cggctgaatg gggcaaaggc
     1981 cggacgtccc tgtccggcct ccacgtcgcg atcgctgatg tcgatcagct cgcgcgcgcc
     2041 aggttgatgc tgaggccctt gaagcctgtg ccgctcgtcg tgatcgagac ggcctgactg
     2101 ctgcccgaga ggttgacgct catcgagccg gtgacgccgc ccgagatcga caggctgatg
     2161 cgattgccgg tcgcctggcc ggtgacgtcg cccgtcgcgt tgaaattgcg ctcctcccag
     2221 ctgcccgaga cgctcgagcc cttgctcagc aggttgccgc gcagctcgat cttggtgccg
     2281 gagctgttgc cgcagcggat ggcgagggtg aggcccgcgc cgctgtcctt tggcgtgtaa
     2341 taggccgtgc agcggatgct ctccgtccgg ccgccgtcga agcgcacctg accgccgccg
     2401 gaccaggagc ccgagaatga gacgaacggg ccggccgcaa gcgcgggtgc cgtcaccgcg
     2461 acatgcaatg tcgcggccgc tgcagcaata gagaggacgc ccggaatggc gcgtcgcaga
     2521 gagagtgtcg acatatcggg tgacccccag cctttcgaaa acgaccgaaa cagttgttcc
     2581 gcgagattgt ctatcacaga tcgcgccatg accgacaggc cttcaagatc caattccgcg
     2641 cctgaatgtg acctgaagga cgacgcatgc ggcccgcgcc tggacgtcag cgcggcacgc
     2701 ctttggcagg taccggtgcg gtcgcggccg gatcaccctt gcgatcgccc cggtcggcgg
     2761 tcgagggaac atactcctcc ttgcggcagg agcagcctgt gacgacctcc ttgcgatact
     2821 tgaaggccga gcgaagcgtg ttgtacggca gctgtgtgcg atgcgagacc atctgctcca
     2881 ccgagccgcc cgggttctga tagtagtaga gctcgaccgg cgcggcgcag cgcgactggc
     2941 agatgtcctg gtccttgtcg aagtggttgc gcagcgtcgc aaagctcacc gggaaatagt
     3001 agccgtcgca gagccgcacg cagagggtgc ggtaggtcgc gtagggcagc gcgccgaagt
     3061 tgccgccgcg tgccgaaggg ccggcatcct cgtcctgcca ccagccggcg aatgggcccg
     3121 tgccgccgcg cttggcggct tcggccgtgt aggtcgagcc gcaattgttg cgggcgagct
     3181 cgcggatcag ctcatcctgc atggagcgct cgccgaggcc taggatctgc tggcgctggg
     3241 cgtcgagctc catcaggcgg cgcttggcat actcgccctg gttgaagagg gcgacgcatt
     3301 cgggcgtgcg ccgcagcgtc ttgttgaaca gccagaactc gtagcactgc gagcgctcga
     3361 gcgaggcgtt cgccgtctgc agctgccgct cgagctggcg catctccaat tcgagcttct
     3421 ggcgcacctc gaccgaggat ttgccgcgct ggccttcggc gatgaggcgc tgctcgagct
     3481 ggaagcagat ctgcgactgg ccggcc
//
LOCUS       DAORNH010000384         3487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_20340_length_3487_cov_2.421911, whole genome shotgun sequence.
ACCESSION   DAORNH010000384 DAORNH010000000
VERSION     DAORNH010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20340_length_3487_cov_2.421911"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>158)
                     /locus_tag="PK264_23810"
     CDS             complement(<1..>158)
                     /locus_tag="PK264_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78929.1"
                     /translation="RARYGIAWGALGAAEFCWHQARQYVLDRKQFGRPLAANQLIQKK
                     LADMQTEI"
     assembly_gap    159..168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<169..>587)
                     /locus_tag="PK264_23815"
     CDS             complement(<169..>587)
                     /locus_tag="PK264_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78930.1"
                     /translation="YGSEEQRKRHLPKLASGEHVGCFGLTEPDHGSDPGSMVTNARKV
                     AGGYVLNGAKTWISNAPVADVAVVWAKADMGDGKKPEIRGFIVERGMKGFSTPKIEGK
                     FSLRASITGSIVLEDCLVPEANLLPNVAGLKGPFGCL"
     assembly_gap    588..645
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<646..946)
                     /locus_tag="PK264_23820"
     CDS             complement(<646..946)
                     /locus_tag="PK264_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRD78931.1"
                     /translation="MQDKTSPRAKASFTWDDPFHLDNQLNEEERLIRDEARKFCADRL
                     MTRVRDAFRNETFDRAVMREMGEMGLLGVTLPEAYGGSGQSYVAYGLVAREVERVD"
     gene            complement(1121..>2140)
                     /locus_tag="PK264_23825"
     CDS             complement(1121..>2140)
                     /locus_tag="PK264_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78932.1"
                     /translation="NYSGGPIPKTAILEGYAADTPLPELMADTGYVHAGKSLLVNRHK
                     NIEAALGWVDDGQEADFEGEFEAAVRQPYQYLLFASEWAPLADELGVSLGGTNRSTED
                     AVALIVLNHAALKNPGKRLLVGLDATSFEDDGFSRTRERAETLGAGLMIYVEGPGGPT
                     GDEWADDEKGRIKMAAKSVGVDVTRTEREWLKVWKNGKWKDYTFKQLAKYKDQGFVAA
                     EIDNLYQAVGESDEAVKLLQLLDEYQAGVQAGHLPRLVLKNLTGPQLRKIAGAIKSGR
                     LSRDTFAEFHIAEKGTGTPSEQKEEAAKIAIRTIRSTNTHQYTAFGAYGLEERFDEMM
                     GLQPA"
     assembly_gap    2141..2150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2151..>3487)
                     /locus_tag="PK264_23830"
     CDS             complement(<2151..>3487)
                     /locus_tag="PK264_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78933.1"
                     /translation="DIWGLSEVSPSAVKALVGELKAQFGENYDFALSEPDAPDSRQTT
                     AVIWKRKTVTGRKEPWPAEIERLWHLDSRDDLEATDGQIFNRYPGLFRFTAESHGHPV
                     EFLLVPLHLKAMDEGQKRRRLASKLLARAVREMRDVHGYSGDWVIGGDFNAELASDDF
                     DSLLGQDFHAMSAEDERQGAMSYIKSPKSLIDHIFLSPNLARLAGDTGFFIVAKEKSV
                     DNYARKLSDHRPVLVRLSLGEPQQHETHEDLDALADKLMAEAHRRAPGNGWRKPARRA
                     LHAAMESHGDALEAATYSFVTSGLDKRAFIERNLDELKRLVGDVNDRLRSDHGADASP
                     VSMLDLAVLLNCEAGLKNGKVDPNHTHSLGERGLFPLPDNLRSFWNGSDAPYPKAPMA
                     LGINIYHFMLYLGRIKNKVYSGMVLYKGHFQHAGIKGVARREARLLAGIVHGY"
BASE COUNT          537 a         1077 c         1137 g          658 t
ORIGIN      
        1 gatctcggtc tgcatgtcgg cgagcttctt ctggatgagc tggttggcgg cgagcgggcg
       61 gccgaattgc ttccggtcga gcacgtactg gcgcgcctgg tgccagcaga actccgccgc
      121 tcccaaggcg ccccaggcga tgccgtagcg tgcgcggtnn nnnnnnnnga ggcagccgaa
      181 ggggcccttc agtccagcta cgttcggcag caggttcgct tccggcacga ggcaatcctc
      241 gagcacgatc gagccggtga tcgaggcacg cagcgagaac ttgccctcga tcttcggcgt
      301 cgagaagccc ttcatgccgc gctcgacgat gaagccccgg atctcgggtt tcttgccgtc
      361 gcccatgtcg gccttggccc agacgacagc gacatcggcg actggtgcat tcgagatcca
      421 ggtcttggcg ccgttgagca cgtagccgcc ggcgaccttt cgagcgttgg tgaccatgga
      481 gccagggtcg gagccgtggt cgggctcggt caggccgaag cagccgacgt gctcgccgga
      541 tgcgagcttc ggaagatggc gtttccgctg ctcctcgctg ccgtaggnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatcg acccgctcca
      661 cctcgcgcgc gacgaggccg taggcgacat aggattgccc cgagccgccg taggcttcgg
      721 gcaacgtcac gccgaggaga cccatttcgc ccatttcgcg catcacggcg cgatcgaacg
      781 tctcgttgcg gaaggcgtcg cgcacgcgcg tcatgaggcg atcggcgcag aatttgcgcg
      841 cctcgtcccg gatcagccgc tcctcttcgt tgagctggtt gtcgagatga aatgggtcgt
      901 cccaggtgaa agacgccttg gcgcgggggg aggtcttatc ctgcatggcc gggttcctgg
      961 gtagcgggtg tttcgcactt gctagagcaa gtcgaacgcc cgctcaaccc tcgcgacaca
     1021 cccggtgcac ggacatgcag ttccgtggga gcgatcgatc acaactcact ggtatggcgt
     1081 ggctttgcgg tcgattggac ctgcatcctt gccgcaagga tcatgccggc tgcaggccca
     1141 tcatctcgtc gaagcgctcc tcgagcccat acgcgccgaa ggccgtgtat tggtgcgtat
     1201 tggtgctgcg gatggtccgg atggcgatct tcgctgcctc ctccttctgc tcggacggcg
     1261 tgccggtacc cttctcggct atatggaact ccgcgaacgt gtcgcgcgag aggcgtccgg
     1321 acttgatggc gccggcaatc ttgcgcagct gcggccctgt gaggttcttg aggacgaggc
     1381 gtggcaaatg cccggcttgg acgcccgcct gatactcgtc gagcaattgg agcaacttca
     1441 cggcctcgtc gctttcaccg accgcctgat agaggttgtc gatctcggcg gcgacgaagc
     1501 cttggtcctt gtattttgcg agctgcttga aggtgtagtc cttccacttg ccgttcttcc
     1561 agaccttgag ccactcccgc tcggttcgtg tgacgtcgac gccgacgctc tttgcggcca
     1621 tcttgatgcg gcccttctcg tcgtccgccc attcatcgcc cgtgggcccg cccgggcctt
     1681 cgacatagat catcaggccg gcaccgagcg tctcggcgcg ctcgcgcgtt cgcgagaatc
     1741 cgtcatcctc gaatgatgtc gcatcgaggc cgaccaggag gcgctttcca ggattcttga
     1801 gcgcggcgtg attgagcact atgagcgcga cggcatcttc cgtgctccgg ttcgtgccac
     1861 cgagcgagac gccgagttcg tcggccagcg gcgcccactc cgaggcaaag agcaggtact
     1921 ggtagggctg ccgcacagcc gcctcgaact cgccttcgaa gtccgcctct tggccgtcgt
     1981 cgacccagcc gagggcggcc tcgatgttct tgtgccggtt caccaacagc gacttgccgg
     2041 cgtgcacgta gcccgtatcg gccatcagct cggggagcgg cgtgtcagcc gcatagccct
     2101 cgagaatcgc cgtcttcggg atcggcccgc cggagtagtt nnnnnnnnnn aagtagccgt
     2161 gcacgatgcc ggcgaggagc cgcgcctcgc gccgtgcgac gcccttgatg ccggcatgct
     2221 ggaagtgccc cttgtagagc accatgccgg agtacacctt attcttgatc cggccgagat
     2281 agagcatgaa atggtagatg ttgatgccga gcgccatcgg ggccttcgga tagggcgcat
     2341 cgctcccgtt ccagaacgat cgcaggttgt caggtagcgg gaagagcccg cgctcaccga
     2401 gggagtgcgt gtggttcgga tcgaccttgc cgttcttgag accggcctcg cagttgagca
     2461 gaacagccaa gtcgagcatc gagacgggtg aggcatccgc gccgtgatcg cttcgcaacc
     2521 ggtcgttcac atcgccgacg aggcgcttca gttcatcgag attgcgctcg atgaaggcgc
     2581 gcttgtcgag gccggacgtc acgaaggagt acgtcgcggc ctcaagggca tcgccgtggc
     2641 tctccatggc ggcatgcagc gcgcgtctcg caggcttgcg ccaaccgttg cccggcgcgc
     2701 ggcgatgcgc ctccgccatc aacttgtcgg cgagtgcgtc gaggtcttcg tgggtttcgt
     2761 gctgctgcgg ctcgccgagc gagaggcgga cgaggacggg gcggtggtca gagagcttgc
     2821 gcgcatagtt gtcgacggac ttctccttgg cgacgataaa gaagcccgta tcgcccgcga
     2881 gccgcgccag gttcggcgag aggaagatgt ggtcgatgag cgacttcggc gacttgatgt
     2941 agctcatcgc gccttgccgc tcgtcctcgg ccgacatcgc atggaagtcc tgcccgagga
     3001 ggctgtcgaa atcgtcggag gcaagctcgg cgttgaagtc gccaccgatc acccagtcgc
     3061 cgctgtagcc gtgaacgtcg cgcatctcgc ggacggcacg tgcgagcagc tttgaggcga
     3121 gccggcgccg cttctggccc tcgtccatgg ccttgaggtg cagcggcacg agcaggaact
     3181 cgaccgggtg gccgtggctc tcggcggtaa agcggaagag gcccgggtag cggttgaaga
     3241 tctgcccgtc ggtcgcctcg agatcatcgc ggctgtcgag gtgccagagt cgctcgatct
     3301 cggcgggcca aggctccttg cggccggtga cggtcttgcg cttccagatg accgccgtcg
     3361 tctgccggct gtcgggcgcg tcgggctcgg agagggcaaa gtcgtagttc tcgccgaact
     3421 gggccttgag ctcgccgacg agcgccttca ccgcgctcgg cgaaacctcg gagagccccc
     3481 agatgtc
//
LOCUS       DAORNH010000385         3424 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_20826_length_3424_cov_2.706144, whole genome shotgun sequence.
ACCESSION   DAORNH010000385 DAORNH010000000
VERSION     DAORNH010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20826_length_3424_cov_2.706144"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1626
                     /locus_tag="PK264_23835"
     CDS             301..1626
                     /locus_tag="PK264_23835"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRD78934.1"
                     /translation="MNEPLKLGIAGLGTVGAGLLSLIEQHGRRLSETVGREIKVVAVS
                     ARDRAKGRGTDIGAFKWFDDATRLAADPGVDVLVELIGGDEGVARKSVEVALAAGKHV
                     VTANKALLARHGVELARLAEKHKVALNFEAAVAGGIPVIKALREALAGNQVRRVYGIL
                     NGTCNFILTKMQDEGRAFGEVLKEAQAKGYAEADPTFDIGGFDTAHKLTLLTSLAFGT
                     KVALDQIHIEGIQTITQADIESAGDLGYRIKLLGVAMLTESGIEQRVHPTLVPKHAAI
                     AEVSGVTNAVAIDGDYVGNLLLVGPGAGAKATASSVMSDIVDIARGGIMPPFSVPSGK
                     LKTYRRARIGQHQGAYYLRLVVQDRAGVMASIAKRMADRDVSLESVVQRRPRAALPGI
                     DARQPPGTPTAVVIVTHETTEEAIRKALEGIESDGILAEKPQMIRIERL"
     gene            1776..2768
                     /gene="glpX"
                     /locus_tag="PK264_23840"
     CDS             1776..2768
                     /gene="glpX"
                     /locus_tag="PK264_23840"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRD78935.1"
                     /translation="MAQDKEKGGLERVLVLEVARVTEAAAIASARWRGKGQSKPADKA
                     AVDAMRRELGKLSIRGVVVIGEGEMDEAPMLYIGEKVGTGDGPEVDIAVDPLEGTTIC
                     AKAMPNSLAVLAISERGGLLHAPDIYMDKIAIGPGYPKGLVDLDKSPVENLAALAKAK
                     GVPVSEITACILDRPRHEKLIKAVRDAGAGVQLIPDGDIAGVIWTTDSAQTGVDIYLG
                     SGRAPEGVLAAAALRCIGGQMQGRLLPENSEQRQRIISMGIKDPARKYAMEEMASADV
                     IFSATGVTGGSLLDGVKFRGEFAETETVVMRSKTGTVRRIKTTFRDIGTKFAAI"
BASE COUNT          616 a         1151 c         1129 g          528 t
ORIGIN      
        1 cgcgtagccg gccggcgccg gctggccaga cctcggacgt cgccttcgcg ccgactacga
       61 cttcgcgggg ccggccggcg cgggccggct gcatctcgga acgcggcgat cgcacgggtt
      121 gcatctcggc gtagctatca cgcgtcatgc gacttgcggg aaagcaaggg gcaccctctg
      181 agtgcgtgga cctgacgagt gtcaccggac aacgagcgag gtgtacccgc gcgaacgccc
      241 gtgttaggag ggcccgcgcg cggcgcgacc gattggcctc ctggacgacg tggcggaagc
      301 atgaacgagc ccttgaaact aggcattgca ggcctcggca ctgttggcgc cggcctcttg
      361 agtctcatcg agcagcacgg ccggcggctt tccgaaaccg tcggccgcga gatcaaggtc
      421 gtggccgtct cggcccgcga tcgcgccaag ggccgcggca cggacatcgg tgccttcaag
      481 tggttcgacg atgcgacgcg gcttgcggcc gatcccggcg tcgatgtcct cgtcgagctg
      541 atcggtggcg acgaaggtgt cgcgcgcaag agcgtcgagg tcgcactcgc ggccggcaaa
      601 cacgtcgtca ccgccaacaa ggcgttgctc gcccgccacg gcgtcgagct tgcccgcctc
      661 gccgagaagc acaaggtcgc gctcaacttc gaggcggccg tcgccggcgg cattccggtc
      721 atcaaggcgc ttcgcgaggc gctcgccggc aaccaggtgc gtcgcgtcta cggcatcctg
      781 aacggcacct gcaatttcat cctgacgaag atgcaggacg agggccgcgc cttcggcgag
      841 gtcctgaagg aagcgcaggc caaaggctac gccgaggccg acccgacctt cgacatcggc
      901 ggcttcgaca ccgcccacaa gctcaccctg ctgacgagcc tcgccttcgg caccaaggtc
      961 gccctggacc agatccatat cgagggcatc cagaccatca cgcaagctga catcgagtcc
     1021 gccggcgatc tcggctatcg catcaagctg ctcggcgtcg ccatgctcac cgagagcggc
     1081 atcgagcagc gcgtccaccc cacactggtg ccgaagcatg cggccatcgc cgaggtctcg
     1141 ggcgtcacca atgcggtagc gatcgacggc gactacgtcg gcaaccttct cctcgtcggc
     1201 cccggcgcgg gtgcaaaggc cactgcctcg tcggtgatga gcgacatcgt cgatattgcg
     1261 cgcggcggaa tcatgccgcc cttttcggtg ccctcgggca agctcaagac ctaccgccgc
     1321 gccaggatcg gtcagcacca gggcgcctat tatctccgcc tcgttgtcca ggatcgggcc
     1381 ggcgtaatgg cctcaattgc gaagcgcatg gccgaccgcg acgtctcgct cgagagcgtc
     1441 gtgcagcgcc gcccgcgcgc ggctctcccg ggcatcgacg cgcgccagcc gcccggtacg
     1501 ccgaccgccg tcgtgatcgt cacacacgag acgaccgagg aggcgatcag gaaggcgctc
     1561 gagggcatcg agagcgacgg catcctcgcc gagaagccgc agatgatccg catcgagcgg
     1621 ctctgacact cgcccgtcat gctgcggcgc gagatcacga tgtgcaccct cggcgcgacc
     1681 gcgctgcgag cggggctcga cacgtctatt ctgccaaaga tgcagcccgt tcaacatcgg
     1741 ccggcggaca ggccacgaga cgaggaggac agacgatggc gcaggacaag gaaaagggtg
     1801 ggctcgagcg cgttctggtg ctcgaggtcg cgcgcgtcac ggaagcggcg gccatcgcct
     1861 cggcgcgctg gcggggcaaa gggcagagca agcctgccga caaggccgcc gtcgacgcca
     1921 tgcgccgcga actcggcaag ctctcgatcc gcggcgtcgt cgtgatcggt gaaggcgaga
     1981 tggatgaggc gccgatgctc tacatcggcg agaaggtcgg cacgggggac ggccccgagg
     2041 tcgacattgc tgtcgatccg ctcgagggca cgacgatctg cgccaaggcg atgccgaact
     2101 cgctcgctgt cctcgcaatc tcggagcgcg gcggcctcct gcacgccccc gacatctaca
     2161 tggacaagat cgcgattgga cctggctatc cgaagggcct cgtcgacctc gacaagtcgc
     2221 cggtcgagaa ccttgctgcg ctcgccaagg cgaagggcgt cccggtgagc gagatcaccg
     2281 cctgcattct cgaccggccg cggcatgaaa agctcatcaa ggccgtgcgc gacgccggcg
     2341 ccggcgtgca gctcattccc gatggcgaca ttgcgggcgt gatctggacg accgattcgg
     2401 cgcaaacagg cgtcgacatc tacctcggct cgggccgcgc ccccgaaggc gtgctcgcgg
     2461 cggcggcact gcgctgcatt ggcggccaaa tgcagggccg cctcctcccc gagaactcag
     2521 agcagcgcca gcgcatcatc tccatgggta tcaaggatcc cgctcgcaag tacgcgatgg
     2581 aggagatggc ctccgccgac gtcatcttct cggcgacggg cgtcaccggc ggctcgctgc
     2641 tcgacggcgt gaagttccgc ggcgagttcg ccgagaccga gaccgtcgtg atgcgctcga
     2701 agaccggcac tgtgcgccgc atcaagacga cgttccgcga cataggcacg aagttcgcgg
     2761 cgatctgagc atcgcggcgc ccggcgccat gcaacgccgg gcgctgctgc cgcatctcga
     2821 cgccgcatcg cgacagtggc ttcgccaatg cgcagccctc gagtcgctcc gctgcgcgca
     2881 tgtgggccgc cactgatggc gtacgacagg ccagtgtctc ggttcggaca ttcttatgca
     2941 cgtggcagca cactcgggat acagactacc gaacatgatg tcgcaccgcg ctcatggaag
     3001 cgttcgtatc cctgccgatc gaagggttcg tctgtgcttg ctgaagatgg cgatgggctg
     3061 cgcgatcgga attccgggcc aagcgcggcc cgaggtcgaa ggccgggacg cggcaggaca
     3121 ggtgtcaacg caatctgcag gcagaggtgc tcttcctcat gctcctcgct ccacagatgg
     3181 attcaactat cacgtggagg gaaatccgga gagcaggtca gcgtcagtgc cgttcacgca
     3241 agtcgattgc cggctctcga acgggcctct ccaaaaggca gggcagatgt gaaaaataat
     3301 ccttcacaag cccttcccaa cgcgccggcc agtagttata agcccacctc ttgctgcaac
     3361 atgcagcgct cggattggtg acgcggggtg gagcagcccg gtagctcgtc aggctcataa
     3421 cctg
//
LOCUS       DAORNH010000386         3374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_21193_length_3374_cov_2.618861, whole genome shotgun sequence.
ACCESSION   DAORNH010000386 DAORNH010000000
VERSION     DAORNH010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21193_length_3374_cov_2.618861"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK264_23845"
     CDS             complement(<1..260)
                     /locus_tag="PK264_23845"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD78936.1"
                     /translation="MIAAANDRLFVKLCDVLARPDLGTDARYRTNRLRVENKGSLLPV
                     IQEVLAAEPAEVWLERLEAARIPCGPIHDMKSMDSEPHVAALG"
     assembly_gap    268..315
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<316..987)
                     /locus_tag="PK264_23850"
     CDS             complement(<316..987)
                     /locus_tag="PK264_23850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRD78937.1"
                     /translation="MTPLAGIKVIEIAQNIAGPYAGDILAHLGADVIKVERPGGGDDA
                     RGWGPPIDGDTATTFLAINRGKRSITVDFKDAEALARLKSLIAEADVLVQNMRPGSLE
                     EAGLGADELLALNPRLVYASLSAYGHKGPKRLDPGYEPILQAFSGMMMMNGEDGGPPT
                     RVGMQVLDLGTGVWTALGVLAALMRRQQSGKGGTVDTSLLETALGWMGCHFAGYSVTG
                     RPPRRH"
     gene            complement(1005..1334)
                     /locus_tag="PK264_23855"
     CDS             complement(1005..1334)
                     /locus_tag="PK264_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HRD78938.1"
                     /translation="MKMSDIPFGTTDWAKVPETKHAGEKGVATWRTQQFGAIRVRMVE
                     YSPGYLADHWCQKGHILLVLEGRLDTELEDGRRVTLHPGQSYQVADGAEPHRSTTEVG
                     AKLFIVD"
     gene            complement(1331..1579)
                     /gene="yacG"
                     /locus_tag="PK264_23860"
     CDS             complement(1331..1579)
                     /gene="yacG"
                     /locus_tag="PK264_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HRD78939.1"
                     /translation="MGQSRPGPLIDSAPIGGKCPICSGPGMKATRPFCSKRCADVDLA
                     RWLRGAYTIIDDADADEDGDAPELARAVQGKSDGDERS"
     gene            complement(1597..2343)
                     /locus_tag="PK264_23865"
     CDS             complement(1597..2343)
                     /locus_tag="PK264_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HRD78940.1"
                     /translation="MTSLKAGLLTALSRSDRAPRESLRRQRARVKPTGRRAELRPRLV
                     LASASPRRLMLLSQAGVAPDALRPTSIDETPGKREMPRALALRLARAKAEAARDQIAN
                     DKDLADAYVLAADTVVAVGRRILLKPDNVDQAMAALQLLSGRSHRVLTAICLITPEDR
                     LRIKVVDTSVRFKRLSAEEVQSYIASREWRGKAGGYAIQGLAGCFVQKLSGSYTNVVG
                     LPLTEVIGMLLGEGFPIHFNWLKAGEADPE"
     gene            complement(2303..2521)
                     /gene="infA"
                     /locus_tag="PK264_23870"
     CDS             complement(2303..2521)
                     /gene="infA"
                     /locus_tag="PK264_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRD78941.1"
                     /translation="MAKEELIEVSGVISELLPNATFRVKLENGHELIAHTAGRMRKNR
                     IRVLAGDKVLVEMTPYDLTKGRITYRFK"
     gene            2780..3235
                     /locus_tag="PK264_23875"
     CDS             2780..3235
                     /locus_tag="PK264_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRD78942.1"
                     /translation="MREQLNQAVKIAMKAQDKKRLGTLRLIAAAIKDRDLGIGGAAPV
                     SGKTPDADILSLLTKMVKQRRESIKTYEEAGRCELAQQEQSEIAIIEEFLPKQMSEAE
                     TQTAIERLVTELGAAGLKDMGRVMAAAKERFAGKMDFSKASGIVKKALG"
BASE COUNT          582 a         1085 c         1091 g          568 t
ORIGIN      
        1 ccgagggccg cgacgtgcgg ctcggaatcc atgcttttca tgtcgtggat cggcccgcag
       61 ggtatgcgcg ccgcctccag tcgctcgagc cagacctcag caggctccgc cgcgagcacc
      121 tcctgaatta caggcaacaa actgcccttg ttctcgacgc gcagcctgtt ggtgcgatag
      181 cgcgcatcgg tgccgagatc gggtcgggcg agcacgtcgc agagcttcac gaacaggcgg
      241 tcgttcgcgg cggcgatcat gatcggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnatgcc ggcgcggcgg ccgaccggtc acgctgtagc cggcgaaatg
      361 gcaccccatc cagccgagcg ccgtctcgag cagcgaggta tctacggtgc cgcctttgcc
      421 ggactgctga cggcgcatca aggccgcgag cacgccgagg gcggtccaca cgcctgtgcc
      481 aaggtcgagc acctgcatgc cgacgcgagt cggtggaccg ccatcctcgc cgttcatcat
      541 catcatgccg gagaaggctt gcagaatcgg ctcgtagccc gggtcgaggc gcttcggacc
      601 tttgtggccg taagccgaca gcgacgcata gacgagccgc ggattgagcg cgaggagctc
      661 gtccgcgccg aggcctgcct cttccagcga gcccggacgc atattctgga cgaggacgtc
      721 ggcctccgcg atcagcgact tgagtcgggc gagcgcttcg gcgtccttga agtcgacggt
      781 gatcgaccgt ttgccgcgat tgatcgcgag aaaggtcgtc gcggtatcgc cgtcgatcgg
      841 cggaccccag ccgcgggcat cgtcaccccc gcccggtcgc tcgaccttga tcacgtcggc
      901 gccgagatgg gcgaggatat cgccggcata ggggcccgcg atgttctggg cgatctcgat
      961 gaccttgatg ccggccagcg gggtcatggc aagctctccg aggcctaatc gacgatgaac
     1021 agcttggcgc cgacctcggt cgtcgagcga tgcggctcgg cgccatctgc gacctggtag
     1081 ctctgcccgg gatgcagcgt cacgcggcgg ccgtcctcga gctcggtgtc gaggcgacct
     1141 tcgagcacga gcaggatgtg gcccttctgg caccagtggt cggcgaggta gcccggcgag
     1201 tattcgacca tgcgaactcg gatcgcgccg aactgctgcg tgcgccaggt ggcgacgccc
     1261 ttctcgcccg cgtgcttcgt ctcggggacc ttcgcccaat cggtcgtgcc gaacgggatg
     1321 tcgctcatct tcatgaccgc tcgtccccgt cgctcttgcc ctgcaccgca cgggcgagct
     1381 ccggtgcatc gccgtcctcg tcggcgtcgg cgtcatcgat gattgtatag gcccctcgca
     1441 gccagcgggc gagatcgacg tcggcgcagc gcttcgagca gaagggccgc gtcgccttca
     1501 tgccgggccc cgagcagatc gggcatttgc cgccaatcgg cgcgctgtcg atcagtggac
     1561 cgggtcgact ctgccccatg ctcgcctgtc tccaagctat tcgggatctg cttcaccggc
     1621 cttcagccag ttgaagtgga tgggaaaacc ctcgccgagc agcatgccga tgacctctgt
     1681 cagcggcagg ccaacaacgt tggtgtagga gcccgacagc ttctggacga agcagccggc
     1741 gaggccctgg atggcgtagc cgccggcctt gccgcgccac tcgcgcgagg cgatgtagct
     1801 ctggacttcc tctgccgaga ggcgcttgaa gcgcaccgag gtgtcgacca ccttgatgcg
     1861 cagacggtcc tcgggcgtga tgaggcagat cgccgtcagc acccgatgcg atcggcccga
     1921 gagcagctgc agggccgcca tcgcctggtc gacgttgtcg ggcttcagaa ggatgcgccg
     1981 gccaacggcg accacggtgt ccgcggccag cacgtaggca tcggcgagat ccttgtcgtt
     2041 cgcgatctga tcccgtgcag cctcggcttt tgcacgggcg agtctcaagg cgagcgcacg
     2101 cggcatttcg cgtttcccgg gcgtctcgtc gatcgatgtc ggtcggagcg catcgggcgc
     2161 gacaccggct tgcgagagca gcatgaggcg gcgcgggctc gccgatgcaa gtacgagcct
     2221 cgggcgcagt tccgcccggc ggcccgtcgg cttcacacgc gcgcgctgcc gccggagcga
     2281 ctcgcgggga gcacggtccg atctacttaa agcggtaagt aatccggcct ttagtgaggt
     2341 catagggcgt catctcgacc agaactttgt caccggcaag gacacggatg cggttcttgc
     2401 gcatgcgtcc ggccgtatgg gcgatcagct cgtggccatt ctcgagcttg acgcggaacg
     2461 tcgcattcgg cagcagctca gatatgacgc cggaaacctc gatcagctcc tctttcgcca
     2521 taaaccctct ctagagattg tcgaggcggc acattgcgca gacgcacttg cgaaatgcaa
     2581 gaggcaagcg cggaggtgag gctccgcctc acgggtcggc atgccgtagg cccttgaatc
     2641 aggcgctgag ttgcagccat tgacatcgcg aggcacgcga ggcgcgcgcg ccacatatgc
     2701 gctgccgtcg aatcacagca tattgcccca cgctcaccac ggcattacac gagcgcccca
     2761 gcgggcaggg agaacagcca tgcgcgagca actcaatcag gccgtcaaga ttgccatgaa
     2821 ggcgcaggac aagaagcgcc tcggcacgct ccggctcatc gccgcggcga tcaaggatcg
     2881 cgatctcggc atcggcggcg cggcgcccgt gtcaggcaag acgccggacg ccgacattct
     2941 atcgttgctg acgaagatgg tgaagcagcg ccgcgaatcc atcaagacct acgaggaagc
     3001 gggacgctgc gagctcgccc agcaggagca gagcgaaata gcgatcatcg aggagttcct
     3061 gccgaagcag atgagcgagg ccgagaccca gaccgcaatc gagcggctcg tcaccgagct
     3121 cggcgccgcg ggtctcaagg acatgggccg cgtcatggcc gccgcgaaag agcgctttgc
     3181 cggcaagatg gacttctcga aggcgagcgg catcgtgaaa aaggcgctcg gctgagcgag
     3241 cgcctcgtcg tcattcctcg ccgcgccgtt tccccggcat catccggtcg aagacgccgt
     3301 ccttgaagac tccggtgatc ctgaatcgtg gactttcatt agaaaacacg atcacacctt
     3361 ctttttctcg gatt
//
LOCUS       DAORNH010000387         3355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_21360_length_3355_cov_2.505758, whole genome shotgun sequence.
ACCESSION   DAORNH010000387 DAORNH010000000
VERSION     DAORNH010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21360_length_3355_cov_2.505758"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>852
                     /locus_tag="PK264_23880"
     CDS             <1..>852
                     /locus_tag="PK264_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579969.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRD78943.1"
                     /translation="LAAVQMGLIYVNPEGPNGNPDPVASGRDIRETFARMAMNDYETV
                     ALTAGGHTFGKMHGAGPASNVGPDPEAAPIEAMGLGWLSSYKSGKGRDAITSGLEGAW
                     TPNPTKWDMGYFDMLFGYEWELVKSPAGANQWTPKNLKPEHMAPDPADPSKKVPIFFT
                     TADMAMRMDPAYEKISRHFHKNPAEFADAYARAWFKLTHRDMGPKVRYLGPEVPKEDL
                     IWQDPIPAVSHPLIDAKDIADLKAKVLASGLSVTDLVSTAWASASSFRGSDKRGGANG
                     ARLRLAP"
     assembly_gap    853..946
                     /estimated_length=94
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <947..1574
                     /locus_tag="PK264_23885"
     CDS             <947..1574
                     /locus_tag="PK264_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500653.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRD78944.1"
                     /translation="GKKVSLADLIVLGGSAAIEKAAKAGGHDISVPFTPGRMDASAEQ
                     TDAESFAPLEPEADGFRNYAKKKYALSAEAMLVDKAHLLTLSAPEMTVLVGGLRVLGA
                     NHGGTKHGVLTATPGKLTNDFFVNILDMGTAWKPISAENEVFEGRCAKTGAVKWTGTR
                     VDLVFGSNSQLRALSEVYAQSDAKAKFANDFVAAWTKVMNADRYDIAK"
     gene            complement(1668..>3355)
                     /locus_tag="PK264_23890"
     CDS             complement(1668..>3355)
                     /locus_tag="PK264_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRD78945.1"
                     /translation="GVPAALIAAPIAMTALYGLVRIAMVLLTQVREGLFATVAMHAVR
                     KLALDTFEHMHRLSLRFHLERKTGGLTRVLERGRAGIEGITRMTLMTLLPTAVELLLV
                     LGILLWVFDWRYVLLVVAMITAYLWFTFAATEWRVKIRRTMNESDSDANTKAIDSLIN
                     YETVKYFGAERREAERYDKSVAIYEKASIKTYTSLAILNSGQAVIFTLALTLCMIMSA
                     ASVMAGTNTVGHFVMVNALMLQLFIPLNFMGTMYREIRQALTDIERMMDVLELNPEIA
                     DRPGAKPLAVAGGTIRFENVAFHYDKQRPILSGLTFEVPAGKMVAIVGPSGAGKSTIS
                     RILLRFYDVAGGRVTIDGQDIRDVTQVTLRAAIGGIVPQDTVLFNDTIYYNIQYGRPD
                     ASEAEIHEAARLAQIDCFIRALPEGYQTMVGERGLKLSGGEKQRVAIARTILKGPPIL
                     MLDEATSALDSHTEKEIQDALDRVSRNRTTLVIAHRLSTIVHADMILVLDKGELVEQG
                     THGELMTRGGLYASLWNRQRDAERARAELAAALEQERLGQRITDGRLVEVKGG"
BASE COUNT          576 a         1104 c         1045 g          536 t
ORIGIN      
        1 cgctcgccgc tgtgcagatg ggcctcatct acgtcaatcc ggaaggcccg aacggcaatc
       61 ccgatcccgt cgcctccggc cgtgacatcc gcgagacctt tgcgcgtatg gccatgaacg
      121 actacgagac cgtcgcgctg accgccggag gccacacctt cggcaagatg cacggcgccg
      181 gccccgcatc gaacgtcggt cccgaccccg aggccgcgcc gatcgaggcg atgggcctcg
      241 gctggctctc gtcctacaag agcggcaagg gccgcgatgc gatcacctcg ggcctcgagg
      301 gcgcctggac gccgaacccg acgaagtggg acatgggcta cttcgacatg ctcttcggct
      361 acgagtggga gctcgtgaag agccccgccg gcgccaacca gtggacgccg aagaacctga
      421 agcccgagca catggctccg gaccccgccg acccctcgaa gaaggtgccg atcttcttca
      481 cgaccgccga catggccatg cgcatggacc cggcctacga gaagatctcg cgccacttcc
      541 acaagaaccc ggccgagttc gccgacgcct atgcgcgggc ctggttcaag ctcacgcacc
      601 gcgacatggg cccgaaggtg cgctatctcg ggccggaggt gcccaaagag gacctcatct
      661 ggcaggatcc tattccggcc gtgagccacc cgctcatcga cgccaaggac atcgcagacc
      721 tcaaggcgaa ggttctcgcc tccggcctct cggtcaccga cctcgtctcc acggcgtggg
      781 cctcggcctc gagcttccgc ggctccgaca agcgcggcgg cgccaacggt gcccgcctcc
      841 gcctcgcccc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggc aagaaggttt
      961 cgctcgccga tctgatcgtg ctcggcggca gtgctgcaat cgagaaggcg gcaaaggccg
     1021 gcggccacga catctcggtg ccattcacgc ccggccgcat ggacgcgagc gccgagcaga
     1081 ctgacgcgga aagcttcgcg ccgctcgagc ccgaggccga cggcttccgc aactacgcga
     1141 agaagaagta tgcgctctcc gccgaggcga tgctggtcga caaggcgcat ctcctcacgc
     1201 tgtcggcgcc ggagatgacg gtgctcgtcg gcggcctgcg ggtgctcggc gccaaccatg
     1261 gcggcacgaa gcacggcgtg ctcaccgcga cgcctggcaa gctcaccaac gacttcttcg
     1321 tcaacatcct cgacatgggg acggcgtgga agcctatctc ggccgagaac gaggtcttcg
     1381 agggccgctg tgccaagacc ggcgccgtca aatggaccgg tacccgcgtc gatctcgtat
     1441 tcggctcaaa ctcgcagctg cgcgcgctct ccgaggttta tgcacagagc gacgcgaagg
     1501 cgaagttcgc caacgatttc gtcgccgcct ggaccaaggt catgaacgcg gaccgctacg
     1561 acatcgcgaa gtgagccggg ggccacgcga ggccagtagc aacgatacag aaggcggcgc
     1621 ttcggcaacg aggtgccgcc ctctttcggg ttgggtgacc gaccagatca gccacccttc
     1681 acctcgacga gtcggccatc cgtgattcgc tggccgagcc gctcctgctc gagagcggct
     1741 gcgagctcgg cgcgggcccg ctcggcatcg cgctgacggt tccacagcga agcgtagagg
     1801 ccgccccgcg tcatcagctc gccgtgcgtc ccctgctcga caagctcacc cttgtcgagg
     1861 acgaggatca tgtcggcatg cacgatcgtc gacaggcggt gggcgatgac cagcgtcgtg
     1921 cggttgcgcg agacccggtc gagcgcgtcc tggatctcct tctccgtatg actgtcgagg
     1981 gcgctggtcg cttcatccag catcaggatc ggcggcccct tcaggatcgt gcgtgcaatc
     2041 gcaacgcgct gcttctcgcc acccgaaagc ttcaggcccc gctcgccgac catggtctgg
     2101 tagccctcgg gcaatgcgcg gatgaagcag tcgatctggg cgagccgtgc cgcctcgtgg
     2161 atctcggcct cgctcgcatc tggccggcca tactggatgt tgtagtagat cgtgtcattg
     2221 aagagcacgg tatcctgcgg aacgatgccg ccgattgccg cgcgcagcgt cacctgcgtc
     2281 acgtcgcgga tgtcctgccc gtcgatcgtg acgcgcccgc cggccacatc gtagaagcgc
     2341 agcaggatgc ggctgatcgt cgacttgccc gcgcccgagg gtccgacgat cgccaccatc
     2401 ttccctgccg gcacctcgaa cgtcaatccc gagaggatcg ggcgttgctt gtcgtaatgg
     2461 aaggcgacgt tctcgaatcg gatcgtgccg ccggcaaccg cgagcggctt tgcgcccggg
     2521 cggtcggcga tctcgggatt aagctcgagc acgtccatca tgcgctcgat gtcggtcagc
     2581 gcctggcgaa tctcgcggta catcgtgccc atgaagttga gcgggatgaa gagctgcagc
     2641 atgagcgcgt tgaccatcac gaaatgtccg acggtgttgg tgccggccat cacgctcgcg
     2701 gcgctcatga tcatgcaaag ggtcagcgcg agcgtgaaga tcaccgcctg ccccgagttc
     2761 aggatggcga gcgaggtgta ggtcttgatg ctcgccttct cgtagatggc gaccgacttg
     2821 tcgtagcgtt ccgcctcgcg ccgctcggct ccgaagtatt tgacggtctc gtagttgatg
     2881 aggctgtcga ttgccttcgt gttggcgtcg ctgtcgctct cattcatcgt gcggcggatc
     2941 ttcacgcgcc attccgtggc cgcgaacgtg aaccagaggt aggcggtaat catcgcgacg
     3001 acgagcagca catagcgcca atcgaacacc cagagcagaa taccgagcac cagcagcagc
     3061 tccaccgccg tcggcagcag cgtcatcagc gtcatgcgcg tgatgccctc gatgccggcg
     3121 cggccgcgct cgaggacgcg ggtcaaacca cccgtcttgc gctcgaggtg gaagcgaagc
     3181 gacagccggt gcatgtgctc gaaggtgtcg agcgcgagct tgcgcacggc gtgcatggcg
     3241 acggtcgcga aaagtccttc gcgcacctgc gtcagcaaca ccatggcgat ccgcacgagc
     3301 ccgtagagcg ccgtcatggc gatcggcgcc gcgatgagag ctgccggaac gccgg
//
LOCUS       DAORNH010000388         3343 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_21471_length_3343_cov_2.342762, whole genome shotgun sequence.
ACCESSION   DAORNH010000388 DAORNH010000000
VERSION     DAORNH010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21471_length_3343_cov_2.342762"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>280
                     /locus_tag="PK264_23895"
     CDS             <1..>280
                     /locus_tag="PK264_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78946.1"
                     /translation="GFIAGNLAERPSELAAPGELRRSAAFTYRAAPAAPDRLAAGVVL
                     PMPDGGSLIVGQDLEDYRALLSRLHVLLLAGLGAIALLGLGAGHLVSQL"
     assembly_gap    281..382
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <383..>664
                     /locus_tag="PK264_23900"
     CDS             <383..>664
                     /locus_tag="PK264_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HRD78947.1"
                     /translation="DRLSLAINAMLGRIEQLMSALKEVSDNIAHDLKTPLNRLRSRAE
                     AALADPAGDPARKEGLEHVLEDADGVIATFDALLLIARIEAGSVAETFQS"
     assembly_gap    665..692
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            717..>924
                     /locus_tag="PK264_23905"
     CDS             717..>924
                     /locus_tag="PK264_23905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRD78948.1"
                     /translation="MTLAVDAAMPVMASVNRQLIGQAVANLVDNAVKYAKTGAGEAPS
                     ITVSARRAGDMAEIAVADRGPGIRA"
     assembly_gap    925..936
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1129..>3343
                     /locus_tag="PK264_23910"
     CDS             1129..>3343
                     /locus_tag="PK264_23910"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HRD78949.1"
                     /translation="MDDPMKSERPLHARLANAPVVHDADRAGREFADLMARSRGPDGD
                     PTLLALIANPAVERLLRGLFSGSPYLTALASQDDRRLARLLLADPDSHLAAAMAQVVA
                     EVPEAPDMAAAMRRLREFKAEIALLAALADLGGVWPVMRVTAALTDAADAAVSAAVRF
                     LFRQLQRRGDWLTGPEDTTPDVTSGYMVLGMGKYGAHELNYSSDIDLIVFFEPRRARL
                     RDGLDALTVYVRLTRDLVRLLQERTGNGYVFRVDLRLRPDPGATHVAMSTDAALHYYE
                     SFGQNWERAALIKARAVAGDIAAGTSFLKELAPFIWRKYLDFAAIADIHAMKRQIHAF
                     KGFGEIGVAGHNIKLGRGGIREIEFFVQTQQLIAGGRQTDLRTSRTLDALERLVQRGW
                     LKREVASELAESYRFFRHIEHRLQMVLDEQTQTLPEDQEKLVSLARFSGFADLDAFSA
                     ELTRHLVRVQGHYGALFEDVPELTAGTSNMVFAGEADDPQTIEALKRMRFSDPAQVIA
                     TVRGWHHGRYPAVRTPRARELLTEVQPLLIEALADTADPNLAFLSFDRFLANLPSSVQ
                     LFSLLRANPRLLQLIAAIMGSAPRLARILSRRSRVLDAVLDPGFFGAVPEPGRLAELI
                     DADLKSATDLQEALDRARVVGSEQAFLIGVRLLSGTIGADQAGRAYATLAEHLIGALQ
                     QRVAAELARQHGKVAGGAAAVVAMGKLGGREMTASSDLDLIVVYDFDPKAIQSDGP"
BASE COUNT          464 a         1135 c         1057 g          545 t
ORIGIN      
        1 cgggttcatc gccggcaacc tcgccgagcg tccaagcgag cttgccgccc cgggggagct
       61 tcggcgctcg gccgccttca cctaccgggc ggcgcctgca gctcctgaca ggctcgccgc
      121 cggcgtcgta ctgcccatgc ccgatggcgg cagcttgatc gtcgggcagg atctcgagga
      181 ctatcgggca ctcctctctc ggctgcatgt cctcctcctc gccgggctcg gcgcgatcgc
      241 gctgctcggt ctgggggccg gccacctcgt gagccaactg nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nngaccggct gagcctcgcc atcaatgcca tgctcggccg
      421 gatcgagcag ctcatgtcgg ccttgaagga agtgtccgac aacatcgcgc atgacctcaa
      481 gacgccgctg aaccggctgc gcagccgcgc ggaggcggcg ttggcagacc cggccggcga
      541 tcccgcccgc aaagagggtc tcgagcatgt tctcgaggat gccgacggcg tgatcgcgac
      601 attcgacgcg ctgctgctca ttgcgcgcat cgaggcgggc tcagtcgcgg aaacgttcca
      661 gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nngagccggc gatcgaggcg gcaggcttga
      721 cgctcgctgt cgatgcggca atgcccgtga tggcgagcgt caatcgccag ctcattggcc
      781 aggcggttgc caacctcgtc gacaatgccg tcaagtacgc caagaccggt gccggcgagg
      841 cgccgtcgat caccgtctcg gcgcgtcgcg ccggggacat ggcggagatt gccgtcgccg
      901 accggggacc gggtatccgg gccgnnnnnn nnnnnnagcg ggtgctgcga cgcttcgtgc
      961 ggctcgatcc gagccgttcg acaccgggca ccgggctcgg attgagcctc gtcgcggccg
     1021 tctcgcggct gcacggtggc agcgtgcggg tcgaggatca cgcgccgggt cttaaagtgg
     1081 tgcttgccgt gccggcgctc gtcgacggcg gctgaacgag gggtggtcgt ggacgatccg
     1141 atgaaatccg aacgtcccct tcacgcgcgt ctcgcgaacg cgcccgttgt ccatgacgcg
     1201 gatcgcgcag gccgcgagtt cgccgatctt atggcgcgga gccgtggtcc cgacggtgat
     1261 cccaccctcc tcgccttgat cgccaatccc gcggtcgaac ggctgctccg cgggcttttc
     1321 agcggctcgc cctatctgac ggcgctcgcc tcgcaggacg accgccggct ggcgcgtctg
     1381 ctgctcgccg atcccgactc gcatcttgca gccgccatgg cgcaggtggt cgccgaagtt
     1441 cccgaggcgc cggacatggc ggcggccatg cgccggctcc gcgagttcaa ggcggagatc
     1501 gcactccttg ccgcgctcgc cgaccttggc ggcgtgtggc ccgtcatgcg cgtgacggcg
     1561 gctcttacgg atgcagccga cgccgccgtc agcgcggccg tccgcttcct cttccgccaa
     1621 ctgcagcgac gcggcgattg gctcactggc cctgaagaca ccacgcccga tgtgacgtcc
     1681 ggctacatgg tgctcggcat gggcaagtac ggcgcacacg agctcaacta ctcgagcgac
     1741 atcgatctta tcgtcttctt cgagccgcgg cgcgccaggc tccgtgacgg cctcgacgcc
     1801 ctgacggtct acgtccgcct gacacgggat ctggtgcgcc tcctgcaaga gcggaccggc
     1861 aacggctacg tcttccgcgt cgatctgcgt ctgcgcccag atccaggcgc cacgcacgtc
     1921 gccatgtcga cggatgccgc cctccactac tacgagagct tcgggcagaa ctgggagcgc
     1981 gcggccctca tcaaggcgcg cgcggttgcc ggcgacatcg ccgcaggcac ctcgttcctg
     2041 aaggagcttg cccccttcat ctggcgcaag tatctcgact tcgcggcgat cgccgacatc
     2101 cacgccatga agcgccagat ccacgccttc aagggcttcg gcgagatcgg cgttgccggc
     2161 cacaacatca agctcggccg cggcggcatc cgcgagatcg agtttttcgt gcagacgcag
     2221 cagctgatcg cgggcggccg ccagaccgat ttgcgcacgt cgcgaacgct cgatgcgctg
     2281 gagcggctcg tgcagcgcgg ctggttgaaa cgcgaggtgg caagcgagct tgccgagagc
     2341 tatcgcttct tccgccacat cgagcatcgc ttgcagatgg tgctcgacga gcagacccag
     2401 acgctccccg aggatcagga gaagctcgtg tcgctcgcgc gcttttcggg ctttgccgat
     2461 ctcgacgcgt tctcggcaga gctgacgcgc catctcgtcc gtgtgcaggg ccattacggc
     2521 gccctcttcg aggacgtacc ggagctgacg gcaggcacct ccaacatggt gtttgccgga
     2581 gaggccgatg accctcagac gatcgaggcg ctgaaacgca tgcgcttcag cgatcctgcc
     2641 caggtgattg cgacggtgcg cggctggcat catggccgct atccggccgt gcgtacgccg
     2701 cgggcgcgag aactcctgac cgaggtgcag ccgctgctga tcgaggcact cgccgataca
     2761 gccgatccga atctcgcctt cctgtcgttc gaccgcttcc tcgcgaacct tccctcgagc
     2821 gtgcagctct tctcgctgct tcgcgccaat ccgcggctcc tgcagctgat cgccgctatc
     2881 atgggctccg cgccgcgcct cgcccgcatc ctctcgcgcc gcagtcgcgt cctcgatgcc
     2941 gtgctcgatc cgggtttctt cggcgcggtg ccggagcccg gccggctcgc cgagctgatc
     3001 gacgccgacc tcaagagtgc caccgatctc caggaggcgc tcgaccgggc gcgggtcgtt
     3061 ggcagcgagc aggccttcct catcggcgtc cgcctcctct ccggcacgat cggtgccgat
     3121 caggccggcc gcgcctacgc aacgctcgcc gagcacctga tcggcgcgct ccagcagcgg
     3181 gtcgcggccg agctcgcgcg ccagcatggc aaggtcgccg gcggggctgc cgcggtcgtc
     3241 gcgatgggca agctcggcgg gcgcgagatg acggcctcgt ccgacctgga tctgatcgtc
     3301 gtctacgatt tcgatcccaa ggccatccag tcggacgggc cga
//
LOCUS       DAORNH010000389         3340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_21497_length_3340_cov_2.580822, whole genome shotgun sequence.
ACCESSION   DAORNH010000389 DAORNH010000000
VERSION     DAORNH010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21497_length_3340_cov_2.580822"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK264_23915"
     CDS             complement(<1..336)
                     /locus_tag="PK264_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRD78950.1"
                     /translation="MKGIILAGGRGTRLHPMTLVASKQLMPVYDKPMIYYPLSTLMLG
                     GIRDILIISTPEDLPAFERLLGTGQRWGIRFTYKAQPRPEGLAQAYILGAEFVGRSPS
                     ALILGDNIFY"
     gene            674..2203
                     /locus_tag="PK264_23920"
     CDS             674..2203
                     /locus_tag="PK264_23920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HRD78951.1"
                     /translation="MTAPAASGGILSLSGRQWLILLMVQLSNMLFGMTITVANLVLPQ
                     VRGTLSATQDEISWVITLNLVATAVATPMTGWLASRLGWRRMMFGTVLGFTIASFLCG
                     IANSLETLILARVAQGLFGAPIMPMGQAILLATFPRHLQPFALVMWGVGAVFGPVAGP
                     ILGAMATEAYNWRAAFFMIVPPGFLTLACIWFALADHTERSSVQFDWIGFIALSTAIV
                     AAQLIFDRGQRLDWFDSREIVVTALVGGVALWIFVAHCLTADQPFLDPRLLLDRNFSI
                     GLLIAFTMGMLNFTSLVLFPSLLHDLRGYPDSVIGTLIAARGIGNWTAFFFIAQLTRV
                     APRLAIGAGLAIQAAGGYWMSSFDINVTESSIFWSHYLMGLGQSVAFTPMTLLAFATL
                     PRSKITEGSAVFTLMRNFGSSLFISLSVMVLIRSTSINYARLSEFITPYRDAMTLSAL
                     PPSWSFESVTGLARVAGEVQRQAAMIGYINAFHMMAYVAAIAVPLALLMRLPPAADAA
                     K"
     gene            complement(2214..>3340)
                     /locus_tag="PK264_23925"
     CDS             complement(2214..>3340)
                     /locus_tag="PK264_23925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD78952.1"
                     /translation="PVGAWFDNLLGTAPSSSPRTAPKPQIVASLEAPDFDAPPRFIAG
                     LRPFRDGGMRLQAERPIEMADGPRHLIHNYGHGGAGMTLAFGSAELAADLADPIIARE
                     QAEGRTARVAILGAGVIGLTTARAIRRRHPATAITIFARECEPAETTSHKAGGMFAPI
                     SNLGEYHEASQRALLAKVLAGSSRMLVAMSRDRGYAGLGLSRRPTYSIDETGRGAVRV
                     HLGKGTRAVKRYDSWLIDPTLVLPGLKRELQEKGVSFIVRHFADEKEVYQLAQSIIVN
                     CTGLGAKALFGDELVEARRGHLVLLPNPKKLRYLITSWCGAGTRYLFARENDIVIGGS
                     TLRDDLVPEFDPMNPADVRAGERILRSARSLFEPGAIACA"
BASE COUNT          494 a         1104 c         1154 g          588 t
ORIGIN      
        1 gtagaagatg ttgtcgccga ggatcagcgc cgaggggctt cgcccgacga attcggcgcc
       61 gagaatgtag gcctgcgcca gtccctcggg cctcggctgc gccttgtagg taaagcgtat
      121 gccccagcgt tgcccggtac cgaggaggcg ttcgaaggcg gggagatcct cgggcgtcga
      181 gatgattagg atgtcgcgga tgccgccgag catcagcgtc gacagcggat agtagatcat
      241 cggcttgtcg tacaccggca tcagctgttt ggaggcgacg agcgtcatcg gatgcagccg
      301 cgtgccccgg ccacctgcga gaatgatgcc tttcatgcgc gcctcccatg gatcggtccg
      361 gcgcgcattg tgatgagacg acggttgagg gcgcgttaag accagcggat ctgcgagcgg
      421 tgctcgaacc tccgcgcgat gtccgggccg cacaggcgcg tgtcaacgaa tcggggctgg
      481 ccggtttgtg cggattggcc ggagagcgcc cgcgccggac agtctacggc gagccatgcg
      541 ctgggtggcc ggcatggtgg cggatttgcc ggcgctccag ggctggcgac gtgcgacgcg
      601 ggccgccgcg acctgctaga gtcccggtca agacgccaca ccgcacaagt gcgcccttga
      661 ggaacgaccc gagatgaccg cccccgccgc cagcggcggc atcctgagct tgagcggccg
      721 gcagtggctg atcctgctca tggtccagct ctcgaacatg ctgttcggca tgacgatcac
      781 ggtcgccaac ctcgtgctgc cgcaggtgcg cggcacgctc tcggcgacgc aggacgagat
      841 atcgtgggtc atcaccttga acctcgtcgc gacggcggtt gcgacgccca tgacgggctg
      901 gctcgcgagc cggctcggct ggcggcggat gatgttcggg accgtgctcg gcttcaccat
      961 cgcctcgttc ctctgtggca tcgccaacag tctcgagacg ctgatcctgg ctcgggttgc
     1021 gcaagggctc ttcggcgctc cgatcatgcc gatgggtcag gcgatcctgc tcgcgacctt
     1081 tccgcgccac ctgcagccct tcgcgctcgt catgtggggt gtcggcgcgg tgttcggccc
     1141 ggtggccggg cccattctcg gggccatggc aacggaggcc tacaactggc gcgcggcctt
     1201 cttcatgatc gtgccgcccg ggtttctcac cctcgcctgc atctggttcg cgctcgccga
     1261 ccacacggag cgctcgagcg tgcaattcga ctggatcggc ttcatcgcgc tctcgaccgc
     1321 gatcgtcgcg gcacagctga tcttcgatcg tggccagcgc ctcgactggt tcgactcgcg
     1381 cgagatcgtc gtcaccgcgc ttgtcggcgg ggtggctctc tggattttcg ttgcccactg
     1441 cctcacggct gatcagccgt ttctcgatcc gcgcctgctg ctcgaccgca acttctcgat
     1501 cggcctcctc atcgccttca cgatgggcat gctgaacttc acgagcctcg tcctctttcc
     1561 gagcctgctg cacgacctgc gcggctaccc cgacagcgtg atcggcacgc tgatcgccgc
     1621 gcgcggcatc ggcaactgga ccgcgttctt cttcatagcg cagctgacgc gggtcgcgcc
     1681 gcgcctcgcc atcggcgccg gtctcgccat ccaggcagcc ggaggttatt ggatgtcgag
     1741 cttcgacatc aacgtgacgg agagctcgat cttctggagc cactacctga tggggctcgg
     1801 gcagagcgtc gccttcacgc cgatgacgct gctcgcgttc gcgacgctcc cgcgcagcaa
     1861 gatcacggag ggctcagccg tgttcacgct gatgcgcaac ttcggctcga gcctcttcat
     1921 ttcgctatcg gtgatggtgc tgatccgctc gacgtcgatc aactacgcgc ggctctccga
     1981 gttcatcacc ccctaccgcg atgcaatgac gctctccgca ctgccgccct cgtggagctt
     2041 cgagagcgtg acgggcctcg cgcgcgtcgc cggcgaggtg cagcggcagg ccgcgatgat
     2101 cggctacatc aatgccttcc acatgatggc gtatgtggcg gccatcgccg tgccgctcgc
     2161 cttgctgatg cggctgccgc cggctgccga tgccgcgaag tagcgggcgc ggctcacgcg
     2221 caggcaatcg cgccgggctc gaacagcgag cgggcgctcc tcagtatgcg ctcgccggcc
     2281 cgcacgtcgg ccgggttcat gggatcgaac tcggggacga ggtcgtcgcg gagcgtggag
     2341 ccgccgatga cgatgtcgtt ctcgcgcgcg aagaggtagc gggtcccggc gccgcaccag
     2401 ctcgtgatga ggtagcgcag cttcttcgga ttggggagga gcacgagatg accgcgccgc
     2461 gcctcgacga gctcgtcgcc gaagagcgcc ttcgcgccga ggccggtgca attgacgatg
     2521 atcgactggg cgagctggta gacctccttc tcatcggcga agtggcgaac gatgaagctc
     2581 acgcccttct cctgaagctc ccgcttgagg cccggaagca ccagcgtcgg gtcgatgagc
     2641 cagctgtcgt agcgcttgac ggcgcgggtg cctttgccga ggtgcacgcg caccgcgccg
     2701 cggcccgtct cgtcgatcga gtaggtgggg cggcgcgaga ggccgaggcc ggcgtagccc
     2761 cgatcacgcg acatggccac gagcatgcgc gacgatccgg cgagcacctt ggcgaggagg
     2821 gcgcgctgcg aggcctcgtg gtactcgccg aggttcgaga tcggcgcgaa catgccgccg
     2881 gccttgtgcg aggtcgtctc agccggctcg cattcgcgcg cgaagatcgt gatcgccgtc
     2941 gcagggtggc ggcgacggat cgcacgggcc gtcgtaaggc cgatgactcc cgcgccgagt
     3001 atcgcgaccc gcgcggtccg cccctccgct tgctcgcgtg cgatgatcgg gtctgcgaga
     3061 tcggcggcaa gctcggccga cccgaaggcg agcgtcattc cggcaccgcc gtggccgtaa
     3121 ttgtggatga gatggcgcgg accgtcggcc atctcgatcg ggcgctcggc ctgaagccgc
     3181 atgccgccgt cacggaaggg gcgcagaccc gcgatgaagc gcggcggggc atcgaaatcg
     3241 ggggcctcga gcgacgcgac gatctgcggc ttgggcgcag tccgaggcga cgacgatggc
     3301 gcggtgccga ggaggttgtc gaaccaggcg ccgacggggt
//
LOCUS       DAORNH010000390         3307 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_21816_length_3307_cov_1.874846, whole genome shotgun sequence.
ACCESSION   DAORNH010000390 DAORNH010000000
VERSION     DAORNH010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21816_length_3307_cov_1.874846"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PK264_23930"
     CDS             <1..489
                     /locus_tag="PK264_23930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78953.1"
                     /translation="EGLPAATTTALKPSTSRAGSVLDRLKKRLPTKLKMPTWLPDLAV
                     RLAILKKQLENGKRVEEAVIIAEKALAEVARISAALDAGLRITEAELIAARARLETMA
                     ELIEGLAGRVGVIEARTSATERELALHMQRIARLVAIMTRPGCGTYMAFDRRTRRCRD
                     MR"
     gene            complement(571..646)
                     /locus_tag="PK264_23935"
     tRNA            complement(571..646)
                     /locus_tag="PK264_23935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(610..612),aa:Glu,seq:ctc)
     assembly_gap    777..814
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            844..1497
                     /locus_tag="PK264_23940"
     CDS             844..1497
                     /locus_tag="PK264_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HRD78954.1"
                     /translation="MLGLALAAPFLALPGATRAQPAEAPRPIRIIAFGDSLTAGYGLA
                     AADGFAPQLERALKARGHTLTVINAGVSGDTTTTGLQRLDWAIPAGAEAVIVELGAND
                     ALRGADPARARANLDEIVKRIKAKGAEVLIAGMQAPRNLGEAYVAAFDSIYPELAMTH
                     GTLLYPFFLEGVAMRPELNLPDGIHPTAAGIAVIVAGILPKAEALIARVEARRIRGG"
     gene            1531..>2028
                     /gene="thpR"
                     /locus_tag="PK264_23945"
     CDS             1531..>2028
                     /gene="thpR"
                     /locus_tag="PK264_23945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRD78955.1"
                     /translation="MPRLFSGIELPREVRERLAILAVPISGAKWVPAEDLHLTLRFAG
                     DIEPRIARDLAEALSAIEGELLTLRIAGLGTFGSRDQRVIWAGVEDLGKRDGLARLAR
                     HNERAARNVGLPPSPRPWKPHVTLARMRGGRDEDVARLLGEYGAYALEPFTVERFVLF
                     SARPNV"
     assembly_gap    2029..2183
                     /estimated_length=155
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2301..>3009
                     /gene="lexA"
                     /locus_tag="PK264_23950"
     CDS             2301..>3009
                     /gene="lexA"
                     /locus_tag="PK264_23950"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRD78956.1"
                     /translation="MLTAKQKELLLLIHQRLQESGVPPSFDEMKDALELKSKSGIHRL
                     ITALEERGFIRRLPHRARALEIVRLPDHMSGEGGASAAPAPAAMSNVTPLRPERGGGG
                     ATPVSARAQGRPAPASSRSVPDGIGAIPLMGRIAAGVPIEAIQNHADDIQCPPSLLGP
                     GEHFALEVRGDSMIDAGIHEGDIVVIRRTSNADTGDIVVALVEKEEATLKRLRKRGGT
                     VALEAANPRYETRVFSHD"
     assembly_gap    3010..3019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          514 a         1095 c         1030 g          465 t
ORIGIN      
        1 gagggtctgc cggcggccac caccaccgcg ctgaagccca gcacttcgcg cgccggctcg
       61 gtcctcgacc gcctcaagaa gcgcctgccg accaagctca agatgccgac ctggctcccc
      121 gacctcgcgg tgcgtctcgc gatcctcaag aagcagctcg agaacggcaa acgcgtcgag
      181 gaagcggtga tcatcgccga gaaggcgctc gccgaggtcg cccgcatcag tgcagcgctc
      241 gatgccggcc tgcgcatcac cgaagccgag ctgatcgccg ccagggcgcg cctcgagacc
      301 atggccgagc tcatcgaagg cctcgccgga cgtgtcggcg tcatcgaggc ccgcaccagc
      361 gccaccgagc gcgagcttgc cctgcacatg cagcgcatcg cccgccttgt cgccatcatg
      421 acgcgaccgg gctgcggcac ctacatggca ttcgaccgcc gcacccgccg ctgccgtgac
      481 atgcgctgac gacaacggac gccggggcca gcgcgcctcg gcggcccgtt cattttgaaa
      541 cgtgtcggaa gtgctcgata agcgccggaa tggcgccccg tagggggatc gaacccctgt
      601 tttcaacgtg agaggctgac gtcctaggcc actagacgaa cggggcgagc ggcgcgatct
      661 ataggtgagg ggccggcctc gatcaagcga tttatcgctg gctgtatctc gggcccgggg
      721 atgtgccaga gtgcgccaca actcggcccc ctcggcgcgc tcgagtacgt gctgcannnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncacgcc attctccccc gacacgacgc
      841 gaaatgctgg ggctcgcctt ggccgcaccg ttcctcgccc tgccgggcgc cacccgcgca
      901 cagccggccg aggcaccgcg ccccatccgc atcatcgcct tcggcgacag cctgaccgcg
      961 ggctacggcc tcgccgccgc cgacggcttc gccccgcagc tcgagcgcgc cctgaaggcc
     1021 cgcggccaca ccctcacggt catcaacgcg ggtgtctccg gcgacaccac gacgaccggg
     1081 ctgcagcgcc tcgactgggc gatcccggcc ggcgcggagg ccgttatcgt ggagctcgga
     1141 gcgaacgacg cgctccgcgg cgccgacccc gcccgcgccc gcgccaacct cgacgagatc
     1201 gtgaagcgca tcaaggccaa gggcgccgag gtcctgatcg ccggcatgca ggcgccgcgc
     1261 aatctcggcg aggcctacgt cgctgccttc gacagcatct accccgaact cgccatgacc
     1321 cacggaacgc tgctctatcc gttcttcctc gaaggcgtcg ccatgcgccc cgaactcaat
     1381 ctgccggacg gcatccaccc aaccgccgcg ggtatcgcgg tgatcgtcgc cggcatcctg
     1441 cccaaggccg aggcgctcat tgcgcgcgtc gaggcgcggc gcatcagggg cggctgaagc
     1501 ccgaagacga gaaggcgcga ggagatccgc atgccgcgac tgttctcagg gatcgagctg
     1561 ccgcgagagg tccgcgagcg cctcgctatt ctcgccgtgc caatctcggg cgcaaagtgg
     1621 gtgccagccg aggatctgca tctcacgttg cggttcgccg gcgatatcga gccgcggatc
     1681 gcgcgtgatc tcgccgaggc gctctccgcc atcgaaggag agctgctcac gctgcgcatc
     1741 gccggcctcg gtacgttcgg cagccgtgat cagcgcgtga tctgggccgg cgtcgaggac
     1801 ttgggcaagc gcgacggcct cgcccggctg gcacgccaca acgagagggc cgcgcgcaac
     1861 gtcggcctcc cgccgagccc ccggccgtgg aagccgcacg tcaccctcgc gcgcatgcgg
     1921 ggcggacgcg acgaagacgt cgcgcggctc ctcggcgagt atggcgctta cgccctagag
     1981 ccgttcacgg tcgagcggtt cgtgcttttc tccgcgcgtc ccaatgtcnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnngtatcgc attcgatcgc tgctcgcgac gatcggtgcc
     2221 ggccagcgag atgccctcga gtgaattgcg gcagtgaagt gcggcggatt gcgccgggcg
     2281 cgaattggac gggacaggac atgctcacag ccaagcagaa ggagcttctg ctcctcattc
     2341 atcagcgtct tcaggaatcg ggtgttccgc cgtcgttcga cgagatgaag gacgcgcttg
     2401 agctcaagtc caagtccgga atccaccgtc tcatcacggc gctggaggag cgcggcttca
     2461 tccgccgcct gccgcatagg gctcgcgcac tggagatcgt ccgactgcca gaccatatgt
     2521 cgggcgaggg cggggcctcc gctgcgcccg cgccagcagc catgtcgaat gtgacgccgt
     2581 tgcgacccga gcggggaggg ggaggggcga cgcctgtttc ggccagggcg cagggccggc
     2641 cggcgccggc gagttcgcga tcagtccccg atgggatcgg ggcaatcccg ctgatgggcc
     2701 gtatcgccgc tggcgttccg atcgaggcca tccagaacca tgctgacgat atccaatgtc
     2761 cgccgagcct gctcggaccg ggcgagcact tcgcgcttga ggtgagaggc gactcgatga
     2821 tcgacgccgg tattcacgag ggcgatatcg tcgtcatccg gcgcacatca aatgccgaca
     2881 ccggcgatat cgtcgtggcg ctcgtcgaga aggaggaggc aaccttgaag cggctccgaa
     2941 agcgcggtgg caccgtcgcg ctcgaggccg ccaatccgcg ctatgagacc cgcgtgttca
     3001 gtcatgaccn nnnnnnnnng tcgatgtgca gggccgcctc gtcggactgc tgaggcgcta
     3061 ttgatgtgct ggacgctggc ggcgaagcgg cttggattgg cggcgagaaa tgcgcgatcc
     3121 gcccttgccc gtgatcgagc tgtcagggtc gcgcctgcac ggcgacggaa ccgatgccgt
     3181 cgaggctgaa ctcgaagcgc tggcccgcgg cgatcggcaa agtggcgaca acgctccccg
     3241 tgatcacgac catgccggcg cgcatcggcc ggcccgccac aatccagtgc ccccaatcgc
     3301 tgtgcat
//
LOCUS       DAORNH010000391         3245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_22350_length_3245_cov_2.444514, whole genome shotgun sequence.
ACCESSION   DAORNH010000391 DAORNH010000000
VERSION     DAORNH010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22350_length_3245_cov_2.444514"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..823)
                     /locus_tag="PK264_23955"
     CDS             complement(197..823)
                     /locus_tag="PK264_23955"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRD78957.1"
                     /translation="MRFGTVEIARPVSRKGHPELKALPPSVTLTLVEAAEVDPPAGVP
                     IAHWRLLTTHTVADAADARRIVSFYRRRWIIEELFRTLKTKGFDIEALSLVEGGPFEK
                     LVAASLIAAVTVLQLVRERDGAARRPIEDAIDKDEQEALEAVSASLEGKTARQKNPHP
                     KGSLAFAAWVFARLGGWTGYYGKPGPIVMLHGLVQFHAIKHGWTLKNV"
     gene            complement(762..1517)
                     /locus_tag="PK264_23960"
     CDS             complement(762..1517)
                     /locus_tag="PK264_23960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78958.1"
                     /translation="MMDGTLGRFGDRRLERGGGFLHARLVAVGSRGVRVRRLGGNRAG
                     EIRITRFLRNPAVSVEEMIETAAGRTGGRVAGRHVLAIQDTTTVRAEDKGRCIALHPV
                     IAVDAVDGALLGLVDARFFVRTGGKAAQRKDKAFAEKESRRWLDGAERAAGALSGASC
                     VTVVADREGDIYEDFACRPAAVEVLIRAGQAPSPHRWIPSVRARREPSRSGAHEHRAA
                     GGPRPSGAHGGPRLALRHRRDRPPGQPQGPPRT"
     assembly_gap    1639..1826
                     /estimated_length=188
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1827..>2401)
                     /locus_tag="PK264_23965"
     CDS             complement(<1827..>2401)
                     /locus_tag="PK264_23965"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD78959.1"
                     /translation="VEDEEISAFARKAFEKQGMAIRTGTSVERLEPGIASVRARLKRK
                     DGAAEDLTFDRVILAVGITGNVENLGLESLGVRIEKGHIDIDQWCRTNVPGVYAIGDV
                     IGGKMLAHKAEEEGIAAVELMAGQAGHVNYEAIPAIVYTWPEVAAVGRTEEQLTADGV
                     AYRVGKFPFTANSRARCNADADGLVKLLADA"
     assembly_gap    2402..2613
                     /estimated_length=212
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2614..3040)
                     /locus_tag="PK264_23970"
     CDS             complement(<2614..3040)
                     /locus_tag="PK264_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRD78960.1"
                     /translation="MTYDVVVIGGGPGGYVAAIRAAQLGLKTAVIEREHLGGICLNWG
                     CIPTKALLRSSEVLGLIKHAADFGITVSGVSFDVKKIVERSRKVAGRLSGGIGYLLKK
                     NKVTVIDGTARLKAPGFVAVTGKAGAALEDVAAKHIIIAT"
     gene            complement(3061..>3245)
                     /locus_tag="PK264_23975"
     CDS             complement(3061..>3245)
                     /locus_tag="PK264_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78961.1"
                     /translation="ADEDENGADNDAFMDDPHWGWHAAYRLGAKPLFPIQYHRGVLET
                     WAPARKYETTLPKAAE"
BASE COUNT          440 a          999 c          937 g          469 t
ORIGIN      
        1 cgagatcgca ctcaaggagc cgtgtgccct cgatccggcc ttggccctgc ggctgaaagc
       61 cctgccgggg gtcgaggatg tgcaggagat gtgaggcgaa gggggcggcc tcgggctccc
      121 tcgcgcgccc cgtccggtga ctatccggcc gcaaggccgg cccaattgag ccccctcccc
      181 ctgcgggcta caggattcac acatttttta gtgtccaacc atgcttgatg gcgtggaact
      241 gaacgaggcc gtggagcatg acgatggggc cgggttttcc gtaatagccg gtccagccgc
      301 cgaggcgggc gaagacccag gcggcgaagg cgagcgagcc cttgggatgg gggtttttct
      361 gacgcgccgt cttgccttcg aggctcgccg agaccgcttc gagcgcctct tgttcgtcct
      421 tgtcgatagc gtcctcgatc gggcgcctgg ccgcgccgtc acgctcgcgc acgagttgga
      481 gcacggtgac ggcggcaatc aggctcgctg ccaccagctt ttcgaacgga ccgccttcga
      541 cgaggctgag cgcctcgatg tcgaagccct tggtcttcag cgtgcgaaac agctcctcga
      601 tgatccagcg ccggcgatag aacgagacga tccggcgcgc gtcggcggca tcggccacgg
      661 tgtgggtggt cagcagacgc cagtgggcga tcggcacacc cgccggcggg tccacttccg
      721 ccgcctccac cagggtgagc gtcaccgagg gcggcagggc cttaagttcg gggtggccct
      781 tgcggctgac cgggcgggcg atctcgacgg tgccgaagcg caaggcgagg accgccgtgc
      841 gcgccggacg gccgggggcc gccggcagct cgatgctcat gcgcgccgct tcgggaaggc
      901 tcgcggcgcg cccgaacaga cgggatccat cggtgaggac ttggggcctg gccggcgcga
      961 atcagcacct cgaccgccgc cggcctacag gcgaaatcct cgtagatgtc gccttcgcga
     1021 tcggccacga cggtgacgca gcttgccccg gacagcgcac cggcggcgcg ttcggcacca
     1081 tcgagccagc gccggctctc cttttcggcg aaggccttgt ccttgcgctg cgcggccttg
     1141 ccgccggtgc gcacgaagaa gcgcgcgtcc accagcccca gaagcgcacc gtcaaccgca
     1201 tcgaccgcga tcaccggatg caacgcgatg cagcggcctt tgtcttcggc gcgaaccgtc
     1261 gtggtgtcct ggatcgccag cacatgccgc ccggcaaccc gcccaccggt ccgcccggcc
     1321 gcggtttcga tcatctcctc gacgctcacc gccggattgc gcagaaaccg cgtgatcctg
     1381 atctcgcccg cacggttgcc gccaagccgg cgaaccctga ccccgcggct gcccaccgca
     1441 accagccgcg cgtgcaaaaa ccctcccccg cgttccagcc ggcggtcccc aaaccgcccc
     1501 aacgttccgt ccatcatccc ctggccccga ccaatggtat catcttccca gggactcaca
     1561 ccaagaatcg aaacgcaaga cataccaacg ccaagatgtg tgaatgccgt aggactgcga
     1621 ccgggggatc gaggggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnntggc gtcagcgagg agtttgacca agccgtcggc
     1861 atcggcgttg cagcgggcgc gggagttggc ggtgaagggg aacttgccaa cgcggtaggc
     1921 gacgccgtcc gccgtcagct gctcctccgt ccggccgacc gccgcaacct cgggccaagt
     1981 gtagacgatc gccgggatgg cctcgtagtt cacatgcccc gcctggcccg ccatcagctc
     2041 gaccgcggcg atgccctctt cttccgcctt gtgcgccagc atcttgccgc cgatgacatc
     2101 gccgatggca tagacgccgg gcacgttcgt ccggcaccat tggtcaatgt cgatgtggcc
     2161 cttctcgatc ctaaccccga ggctctcgag cccgaggttc tcgacattcc cggtgatgcc
     2221 gacggcgagg atcacgcggt cgaacgtcag gtcctcggcc gcgccgtcct tgcgtttcag
     2281 ccgcgcgcgc acgctggcga tgccgggttc gagccgctcg acggaggtgc cggtgcggat
     2341 cgccatcccc tgcttctcga aggccttgcg ggcgaacgcg gaaatctcct cgtcctcgac
     2401 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggtcgc aatgatgatg tgcttcgccg
     2641 cgacatcctc gagggccgcg ccggctttgc ccgtcacggc gacgaagccc ggcgccttca
     2701 ggcgcgcagt cccgtcgatg acggtgacct tgttcttctt gaggagatag ccgatgccgc
     2761 cagagaggcg cccggccacc ttgcgcgacc gctcgacgat ctttttcacg tcgaaggaaa
     2821 cgccactcac cgtgatgccg aagtcggcgg cgtgcttgat gaggcccagg acctcgctcg
     2881 agcgcagcag cgccttggtc gggatgcagc cccaattgag gcagatcccg cccaggtgct
     2941 cgcgctcgat caccgcggtc ttgaggccga gctgggccgc gcgaatggcg gcgacgtagc
     3001 cgccggggcc accgccgatg acgacgacat cgtaggtcat ggggtctcca cccgctcgcg
     3061 ttattcagcc gccttcggca gagtcgtctc gtacttcctg gcgggcgccc acgtctcgag
     3121 cacgccgcgg tgatactgga tcgggaacag cggctttgcg ccgagccggt aggccgcgtg
     3181 ccagccccag tgcgggtcgt ccatgaaggc gtcgttgtcg gcgccgttct cgtcctcgtc
     3241 ggcgg
//
LOCUS       DAORNH010000392         3239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_22406_length_3239_cov_3.016018, whole genome shotgun sequence.
ACCESSION   DAORNH010000392 DAORNH010000000
VERSION     DAORNH010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22406_length_3239_cov_3.016018"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PK264_23980"
     CDS             complement(<1..221)
                     /locus_tag="PK264_23980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRD78962.1"
                     /translation="MPRLLVVTAAALTIGAAFALYSLALETRRLEVAVQARERDIERL
                     EGDIAVLAAERTYLARPERIYALARDLGL"
     gene            complement(255..1253)
                     /gene="rsmH"
                     /locus_tag="PK264_23985"
     CDS             complement(255..1253)
                     /gene="rsmH"
                     /locus_tag="PK264_23985"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRD78963.1"
                     /translation="MTDDGAEKAAFSSSGSPRHVPVLLSEVLAALAPRAGDTYIDGTF
                     GAGGYTSAILAAADCRVVAIDRDPAAIAAAAPLTAEFAGRLTLVQGTFGDLEAIARES
                     GIPRLDGVVLDIGVSSMQLDEAARGFSFMRDGPLDMRMSQAGPSAADMINTSEERELA
                     DILYEYGEERRSRAIARAIVRERAKAPITTTLALARLIESVLGRPRGDDKHPATRTFQ
                     ALRIHVNDELGELERALDAAERMLAPGGRLVVVAFHSLEDRIVKRFLARRAGREGRGS
                     RHMPVANVAEREPSFRILNQRPIHAREAEIATNPRSRSAVLRAAVRTSAPAFPHES"
     gene            complement(1250..1804)
                     /locus_tag="PK264_23990"
     CDS             complement(1250..1804)
                     /locus_tag="PK264_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRD78964.1"
                     /translation="MDRFVSTFTNRIDAKGRVSVPAPFRAVLDRDRYASGGAQGIYCY
                     PSLDTPALDAGGERLARKIDALLGALPDYSDERDELSVALYGDVQVLSIDGDGRIVLP
                     QGLREHAGLLAEVTFVGLGDKFQMWEPKRFDERRLAARAKVHEHRRLFGAGSRQGEPG
                     GSGGDGHSPTTPPGSPSDGGAPER"
     gene            2258..2479
                     /locus_tag="PK264_23995"
     CDS             2258..2479
                     /locus_tag="PK264_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78965.1"
                     /translation="MAAFPAGARELRLIAPHRDCALSLFRYARGLFQWRVFGGPMLQA
                     VLVLLVAILGLGALPARADDLAIVIANKA"
     gene            2510..2794
                     /locus_tag="PK264_24000"
                     /pseudo
     CDS             2510..2794
                     /locus_tag="PK264_24000"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
     gene            2819..>3239
                     /locus_tag="PK264_24005"
     CDS             2819..>3239
                     /locus_tag="PK264_24005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78966.1"
                     /translation="MAEGAEIILVLEACFSGRSSKGPVQPKVSGSAFVVELGPDASIL
                     TLAATGADHVASWDLEEKQGLFTGLLVETLFGRAGRERDGRITVEHLSAHIDREMGAR
                     LARLYPGESRSQRPWLGGRTTAPALIAHTEPLPPLRPD"
BASE COUNT          487 a         1109 c         1107 g          536 t
ORIGIN      
        1 ttgaggccga gatcgcgtgc tagcgcgtag atgcgctcgg ggcgggcaag ataggtgcgc
       61 tctgcggcga gcacggcgat atcgccttcg aggcgctcga tgtcgcgctc gcgcgcctgc
      121 accgcgacct cgagccggcg cgtttcgagc gccaggctgt agagcgcgaa ggccgcgccg
      181 atcgttaggg ctgcggcggt gaccacgagc agtcgcggca tgtcgcctcc atcggcacca
      241 gcatgtggcg cggctcagga ctcatgcgga aacgccggcg cgctggtacg cacagcggcg
      301 cgcaaaaccg ccgagcgcga acgcggattg gttgcgattt ccgcctctct cgcatgtatc
      361 gggcgttggt taagaattcg gaaactcggc tcgcgctctg cgacgttcgc aactggcatg
      421 tggcgcgagc cgcggccctc gcggcctgcc cgtcgcgcga ggaagcgctt cacgatccgg
      481 tcctcgagcg agtggaaggc gacgacgacg aggcgcccgc ccggcgcgag catccgctcg
      541 gccgcatcaa gtgcgcgttc gagctcgccg agctcgtcgt tgacgtggat gcgcagcgct
      601 tggaaggtgc gcgtcgccgg atgcttgtcg tctccacgcg ggcggccgag cacgctctcg
      661 atgagccgtg cgagggcgag tgtcgtcgtg atcggcgcct ttgcccgctc acgcacgatg
      721 gcgcgggcga tggcgcgcga gcggcgttcc tcgccatatt cgtagaggat gtcggcgagc
      781 tcgcgctcct cgctcgtgtt gatcatgtcg gcggccgatg gccccgcctg gctcatgcgc
      841 atgtcgagcg ggccgtcgcg catgaaggag aagccacgcg cggcctcgtc gagctgcatc
      901 gaggagacgc cgatgtcgag cacgacgcca tccaggcgcg ggatgccgct ttcgcgcgcg
      961 atcgcctcga ggtcgccgaa ggtgccttgg acgagcgtca ggcggccggc gaactctgct
     1021 gtcaggggtg cggctgccgc gatcgccgcc gggtcgcggt cgatggcaac gacgcggcaa
     1081 tccgcagctg caagaattgc cgatgtgtag cccccggcac cgaacgtgcc gtcaatatag
     1141 gtgtcacccg cccgcggcgc gagcgccgcg agcacctcgg ataagagcac gggaacgtga
     1201 cgcggcgacc cgctactgga gaaggccgcc ttctctgctc cgtcgtccgt catcgttccg
     1261 gtgctccgcc gtcagacgga gagcctggcg gcgtggtcgg tgagtggccg tcacctccac
     1321 tgcctccggg ctcgccctga cggctccccg cgccgaaaag ccgtcgatgc tcgtggacct
     1381 tggcgcgtgc ggccaggcgc cgctcgtcga agcgcttcgg ctcccacatc tgaaacttgt
     1441 cgccaaggcc gacaaaagtg acctcggcga gaaggcccgc atgctcgcga aggccttgag
     1501 gaaggacgat gcgtccgtcc ccgtcgatcg acaagacctg gacgtcgccg tagagcgcga
     1561 cagagagctc gtcccgctcg tccgaatagt ccggcagagc gccgagaagc gcgtcgatct
     1621 tgcgggcgag cctttcgccg ccagcatcga gggccggagt atcgagcgag gggtagcagt
     1681 agatgccttg ggctcccccg ctcgcatagc ggtcgcggtc gaggacggcg cggaacgggg
     1741 ccggcacgga gacgcgaccc ttggcgtcga tcctgtttgt gaatgtcgag acaaagcggt
     1801 ccatctccct ggctgtgctc gcttctcgct acgccctgac cgacccgacc aaccgctacc
     1861 tgtcgcaaag cgcgacccgt ctcaactgaa gtccactcgc gagagcggaa gagggccgcg
     1921 ccacgcctcc gccgatccct tcgtgtcccc gcgtgctccg tcgagcagat ggtggacctc
     1981 gtctcatgtc cgcggctcgg accgcctgca aaattcactc gcgccactcc gccggttcgg
     2041 gaccggggcg gtcgctcgat ccccgccctc agaggatctg cgccgcggcc ccgttctcgg
     2101 cccggtgcgg acgggcattg atgggatgcc atgggaattt atgggtgtca atggattatg
     2161 gccgaaacat ggggaaaagt cggtgagatc ggttggcgac tgcgatgcgt atctcctcgg
     2221 gcgagaatcg aggctctcga attgatcgcc gttctccatg gctgccttcc cggcaggtgc
     2281 gcgcgaactg cgcctcatcg cgcctcaccg cgattgcgcg ctctcgctct tccgctatgc
     2341 tcgcggtcta tttcagtggc gcgtcttcgg aggccccatg ttgcaggccg tgctcgtctt
     2401 gctcgtcgcc attctcggcc tcggcgcgtt gcccgcgcgc gccgacgacc tcgcgatcgt
     2461 catcgccaac aaggcctaaa agaacagcca cgaggtcgag ttcgcgcaca acgatgccga
     2521 gcgtttcgcc gatgccgcgc ggcgtgcgct gcgcatcccc gagagccgcg tcgtcgtcta
     2581 tcgcgagcag acgctcgccg acatgcgcgg cctcgcctcg cgccgcatcg cgagccaggt
     2641 gacgaagcgc accaagcggc tctgggtcta ctactccggc cacggcttcc cgctgatcga
     2701 gggcgggcgg gcgccggagc ccgacctcct ccccgtggac ggcgatccgg gcctgctgaa
     2761 ggaaaccgcc atcgcgctcg acgacttcaa gtagcagttg ggcgccttga agcgctcgat
     2821 ggccgagggc gccgagatca ttctcgtgct cgaggcctgc ttctcgggcc gctccagcaa
     2881 aggtccggtg cagccgaagg tgagcggctc cgccttcgtc gtcgagctcg ggccggatgc
     2941 ctcgatcctg accctcgcgg cgaccggcgc cgatcacgtc gcttcatggg atctcgagga
     3001 gaagcagggg ctcttcacgg ggctcctcgt cgagacgctg ttcggccgcg ccggccgaga
     3061 gcgcgacggg cgcatcacgg tcgagcatct ctcggcccac atcgaccgcg agatgggggc
     3121 gcgcctcgca cgcctctatc cgggcgagag ccggtcgcag cgtccttggc tcggcggtcg
     3181 cacgacggcg ccggccctga tcgcgcacac agagccgctg ccgccgctcc gcccggacg
//
LOCUS       DAORNH010000393         3206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_22687_length_3206_cov_2.639480, whole genome shotgun sequence.
ACCESSION   DAORNH010000393 DAORNH010000000
VERSION     DAORNH010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22687_length_3206_cov_2.639480"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..793
                     /locus_tag="PK264_24010"
     CDS             134..793
                     /locus_tag="PK264_24010"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRD78967.1"
                     /translation="MTGTRSDAGVDRARRSEPRGPQLCLELALVDDLAVWLSVANESA
                     GPIASLILTVSDPGAAAAAASAVQAAQKLGIAALIRGNARLARTVRADGVHVECGDGF
                     EERLEEAREIVGGRAIVGVDAGGSRHDAMTAGERSADYVAFARRGAPEGLDDQCDLVD
                     WWSEIFEPPVVALGVDGPDAATVLAEAGADFLSVTAEGCSSAEEFGTRLTLIRTAIAG
                     G"
     gene            1235..1864
                     /locus_tag="PK264_24015"
     CDS             1235..1864
                     /locus_tag="PK264_24015"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRD78968.1"
                     /translation="MRSACCSRKGGVERDRTKAATYFEIAAARGHALANYNLGLLFLR
                     GDGKPENPIRAYRHILYAAEQGVVAAQYDVGTLYATGAGTDPNAFEAAKWIGRAAARG
                     HLDAEVEYALILIKGHGTPPDPKRAVEFLRSAANKGNVIAQNRLARCYAAGLGVEKSI
                     PTALAWHLVAKAGGKDDDILEAMLKVLPRTDRAAVEKVAAELREKLAVQ"
     gene            complement(1918..2307)
                     /locus_tag="PK264_24020"
                     /pseudo
     CDS             complement(1918..2307)
                     /locus_tag="PK264_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
     gene            2631..>3206
                     /gene="ccsA"
                     /locus_tag="PK264_24025"
     CDS             2631..>3206
                     /gene="ccsA"
                     /locus_tag="PK264_24025"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRD78969.1"
                     /translation="MLEAAEAILVRDGIAALTLRAAARAAGVSHAAPKNHFGDLSGLL
                     SELAAVGYRRLADMMQAASGGAAASAGAGRAYVKFAIANPGLFQLMFRGERLDYGRPA
                     LKEASDRAFGFLMCDPVPRDGAEALDLDAAAGILQRWSAVHGLAMLLITGAIWANESW
                     GRPWGFDSKETGALVAWLTYAAFLHTRISRGW"
BASE COUNT          509 a         1074 c         1136 g          487 t
ORIGIN      
        1 gtacgggcgc cccggcgagc gatgccaagc cggcgccggc tgagagtgac gcgcccgccg
       61 ccagcaccgg ccccgagccg atccctgcga acccaggcgc caaggtcgtg cgcctcgata
      121 cgttccgcaa gaaatgaccg gcaccagatc ggatgccgga gttgaccgtg cacggcggag
      181 cgagccgcgc ggtccccaac tctgccttga actcgcgctg gtggatgatc tcgcggtgtg
      241 gctgagtgtc gcaaacgaaa gcgcgggccc catcgcctcg ctgatcctca ccgtgagcga
      301 tccgggtgcg gccgccgctg ctgcctcggc cgtacaggcg gcgcagaagc tcggcatcgc
      361 ggctctcatc cgggggaacg ctcgcctcgc ccgcaccgtc cgggccgatg gcgtgcacgt
      421 cgaatgcggg gacggtttcg aggagcgtct cgaggaggcg cgcgagatcg tcggcgggcg
      481 ggcgatcgtc ggcgtcgatg ctggtggctc gcggcacgac gccatgaccg ccggcgagcg
      541 aagcgcggac tacgtcgcct tcgcccggcg cggcgcgccc gagggcctcg atgatcagtg
      601 cgatctcgtc gactggtggt cggagatatt cgagccgccg gttgtcgcac tcggcgtcga
      661 tggcccggat gcggccacag tcctggcaga ggcaggggca gacttcctga gcgtgacggc
      721 agagggctgc agtagtgccg aagagttcgg cacgaggctc accctcatca ggacggcgat
      781 cgccggcggc tgatgccggt ctcgtcgatg acaatcgccg gcgatgacga ggaggctgcg
      841 atgacaggac gcgtgctccg gccacggcgg agcgtgccgg gcgttgcctt tgctctgggg
      901 ctcgggttga ccatttcgct ctccgccgtg agcgcggccg tggcccaggt cgcaccctcg
      961 acgccggcgc cgaaatccgg acctgtcatc ctgccctctg ttccggcgcc tcccgacagg
     1021 acgacagacc ctgacgccgc ctacacggcc ttcgagcagg gtcgctacct gatggcgctc
     1081 gcgctcgcga ccaaggccgc cgaggcgggc gagccccagg cgcataccct gatcggccgc
     1141 ctccacgccg agggcctcgg tgtgccacgc aatgagacga ttgcctcgca gtggtaccat
     1201 cgcgcggccc agctcggcga catcgaaggc gcatttgcgc tcggcgtgct gctcgcggaa
     1261 gggcggggtc gagcgcgacc ggacgaaggc cgcgacctac ttcgagatcg cggcggcgcg
     1321 tggccatgcg ctcgccaact acaatctcgg cctgctgttt ctccgcggtg acggcaagcc
     1381 cgagaacccg atccgggcct atcgccacat cctctatgcg gccgagcagg gcgtcgtcgc
     1441 cgcccagtac gatgtcggca ccctctatgc gacgggcgcg gggactgacc ccaatgcctt
     1501 cgaggcggca aagtggatcg ggcgggccgc cgcgcgcggc cacctcgatg ccgaggtcga
     1561 gtacgcgctg atcctgatca aggggcacgg cacgccgccc gatccgaagc gggccgtcga
     1621 gttcctgcga tcggccgcca acaagggcaa cgtcatcgct cagaacaggc tggcgcgctg
     1681 ctacgcggcg ggtctcggcg tcgagaagag catcccgacg gcgctcgcct ggcacctcgt
     1741 cgcgaaggcc ggcggcaagg acgacgacat cctcgaggcg atgctgaagg tgctgccgcg
     1801 cacggatcgc gctgccgtcg agaaggtggc tgcggaactg cgcgagaagc tcgccgtcca
     1861 gtgagcctgc atcggcccac caagagccac ccggctagct tccggggtcc gctttgcgaa
     1921 caaggcccgg tgcaggacgc ggcccggcag cagcgtgaag agcccagcca cgacgagcgc
     1981 acccgcgaat agcatcagca tcgcgcgctt gtgcgcgcga acgttgtgcc ggcgcgcgtg
     2041 gcggacggcg agcacgagcg cggcgagcgt aaagatcgag aggaggtgga tcgggctcca
     2101 ggggccgacg acacgcagct cctggatcag gaacgagctc gcagatacga tgaccatcat
     2161 cgcgacccag atccagccga gcagcctgtg cggcaccgtc cccttgggcg cggcgagctg
     2221 gacgatgccg agaccgaggg caccgagcgc cgccgcaacg tggatcttga tcggcagcga
     2281 tgccgcgagc agtgggtcga gcgtcatggc ggcatctcct tctgatgtga atgcgagtgt
     2341 tcgccgtcgg ctgattgcgc ggccggcggt tcgcgcgtag aattgacatc gtcaatatga
     2401 cgctagcctc atatgttgtc gtcgtcaata ttaatgttga caagggaaag atgtcggtgg
     2461 cggacgcacc acgcaaaggc cgaggtagca acgcttcgcg aggcggacgg gagctcgggc
     2521 cagggcggcg ctctcgcgcg ctccgggcgg gtgagccggc tgctgcccgc acagcggcta
     2581 cttcggacgc acgctcggcg ccctaccatc acggctcgct ccgcgcgagc atgctggaag
     2641 ccgccgaggc aattctcgtt cgcgatggga ttgccgcctt gacgctgcgg gcggcggcgc
     2701 gcgcagccgg tgtctcgcac gccgcgccga agaaccattt cggcgatctg agcgggcttc
     2761 tcagcgagct cgcagcggtt ggctatcggc gcctcgccga catgatgcag gccgcgagcg
     2821 gcggcgcggc cgcatccgct ggagccgggc gcgcctacgt gaagtttgcg atcgcgaacc
     2881 cgggcctttt ccagctcatg ttccggggcg agcggctgga ctacggacgc ccggccctga
     2941 aggaggcgtc cgaccgggct ttcggattcc tgatgtgtga tcctgtcccc cgcgatggcg
     3001 cggaagcgct cgacctcgac gccgctgccg gcatcctcca gcgctggtca gctgtccacg
     3061 ggctcgcgat gctgctgatc acgggtgcta tctgggccaa cgagtcgtgg ggccggccat
     3121 ggggctttga ctcaaaggaa accggtgccc tggtcgcatg gctgacctat gccgcatttc
     3181 tgcacacgcg gatctcacgt ggttgg
//
LOCUS       DAORNH010000394         3201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_22739_length_3201_cov_2.366497, whole genome shotgun sequence.
ACCESSION   DAORNH010000394 DAORNH010000000
VERSION     DAORNH010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22739_length_3201_cov_2.366497"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..>250
                     /locus_tag="PK264_24030"
     CDS             15..>250
                     /locus_tag="PK264_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78970.1"
                     /translation="MTVHVLQQAAAPRLRPRLQWTAGHDRHPPRRHAGRPVGGPARRA
                     AQIRGDLMPKFAANLSFLFTELPFLDRFEAAAKAG"
     assembly_gap    251..332
                     /estimated_length=82
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <333..944
                     /locus_tag="PK264_24035"
     CDS             <333..944
                     /locus_tag="PK264_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRD78971.1"
                     /translation="QALFNMAHGDWTKGERGMAAIPGREKEFEESVKKALAVAEATGC
                     KRLFAMAGLRHQGAERRAYVANLKTAARMAAASGVTVIIEPINTRDIPGYFLNRTEEA
                     RSIIHEVGAPNLGLQFDLYHRQVMEGDVAMAIREFGPLARHYQIASPPDRGEPDEGEL
                     NYRYLFKLIDESGFDGYVGCEYKPRKSTIAGLGWAKALGVSLG"
     gene            complement(975..2810)
                     /locus_tag="PK264_24040"
     CDS             complement(975..2810)
                     /locus_tag="PK264_24040"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRD78972.1"
                     /translation="MATITYGYSGGATATITIDVLLSAAQQTAAIQSALDAVAGHVGG
                     FVTLSAGEFTVSGTGKASDGALRVGSNTTLTGAGTGATVLKLADGSGAVTGIVRTDSG
                     STLTDGTYQTTHDVVLAGLTIDGNRANSSGDVDGFYSGPKPNSGQFDSNITLDRVEIR
                     NVSRYGFDPHEQTHNLTITNSVAHHNGVDGFTIDFSKGVTLSNNIAYANGRHGFNIVT
                     GSTAVLMSGNEAYGNGGSGIVAQTGDNEIRSWTAHVTITGGSVHDNGRAGIEVRQTTG
                     LVIDGVAITGNQKEGVVLSGVVGAELSGNLISGNGLALAPGAAPVRIEGLLQDFGDTD
                     PLNDRWIATSGVVIDGVAVADPTTSPGAAPYTWQVTDGADTIVGSEGRDAIAAGSGND
                     VVDGRAGADTLYGNDGDDRLNGGADNDKLYGGAGADRLIYTAGLDLLDGGAGKDTAEF
                     SYFGKAVYVNLGQTGIEAYTSGTTRATVANATTAIADLVSVEAVLGTVYDDTIFGNAS
                     ANTLSGSGGADVINGGGGNDILNGGRGNDVFAFNAGWGADRIEDFQRGRDKIDFVGIS
                     GLDAFADLTISSSSTGAVVAYAGQTITLVGVSASALSASDFLFHV"
     gene            2991..>3201
                     /locus_tag="PK264_24045"
     CDS             2991..>3201
                     /locus_tag="PK264_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRD78973.1"
                     /translation="MKHVIDELERRRAVARLGGGQARIDAQHKRGKLTARERIELLMD
                     AGSFEELDMYVEHRSTDFGMEKEKIP"
BASE COUNT          509 a         1029 c         1061 g          520 t
ORIGIN      
        1 tctttcggcg agcggtgacc gtccacgtgt tgcagcaggc cgccgccccg cgcctccgcc
       61 cccgcctcca atggaccgcc ggacacgacc gacacccgcc gcgacgacac gccggtcgac
      121 cggtcggggg accggctcgg cgagcggctc agataagggg cgacctcatg ccgaagttcg
      181 cagcaaacct gtcgttcctg ttcaccgagc tgccgtttct cgaccgcttc gaggcggcgg
      241 cgaaggccgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaggcgct cttcaacatg gcgcacggcg
      361 actggaccaa gggcgagcgc ggcatggccg ccatccccgg ccgcgagaag gagttcgagg
      421 agagtgtcaa gaaggcgctg gccgtcgccg aggccacggg ctgcaagcgc ctttttgcca
      481 tggccgggct gcgccatcag ggtgccgagc ggcgcgccta cgtggcaaac ctcaagaccg
      541 cggcgcgcat ggccgcagcc tccggcgtca ccgtgatcat cgagccgatc aatacgcgcg
      601 acattccggg ctatttcctc aatcgcaccg aggaggcgcg ctcgatcatc cacgaggtcg
      661 gagcccccaa cctcggcctg caattcgacc tctaccatcg ccaggtgatg gagggcgatg
      721 tcgcgatggc gatccgagag ttcggaccgc tcgcccgcca ctaccagatt gcgagcccgc
      781 ccgaccgggg cgagccggac gagggcgagc tcaactaccg ctacctcttc aagctcatcg
      841 acgagagcgg cttcgatggc tacgtcggct gcgagtacaa gccgcgcaag tccaccatcg
      901 cgggcctcgg ctgggccaag gcgcttggcg tcagcctcgg ctgagcggtt cggcgtggaa
      961 ccgctcgcct accgtcacac gtggaacagg aagtccgacg ccgatagagc gctcgcgctg
     1021 acgccgacga gcgtgatcgt ttgcccggcg tacgcaacca ctgcgcctgt ggacgaggac
     1081 gagatcgtga gatcggcgaa ggcatcgagc ccgctgatgc caacgaagtc gatcttgtcg
     1141 cgaccgcgct ggaagtcctc gatgcggtcc gcgccccagc ccgcgttgaa ggcgaacacg
     1201 tcgttgccgc ggcctccgtt caggatatcg ttccctccgc cgccgttgat cacgtcggcg
     1261 ccaccgctgc ctgagagcgt gttggcactc gcattgccga aaatcgtgtc gtcgtagacc
     1321 gtaccgagaa cggcctcgac cgatacgagg tcggcgatgg ccgtcgtggc attggcgaca
     1381 gtcgcgcgcg tcgtgccgga ggtgtaggcc tcgatgccgg tctggccgag attcacgtag
     1441 accgccttgc cgaagtagct gaattcggcc gtgtccttgc cggcgccgcc gtcgaggaga
     1501 tcgaggcccg ccgtgtagat gagccgatcg gcaccggcgc cgccatagag cttgtcgttg
     1561 tcggcgccgc cgttgagacg atcgtcgccg tcgttgccgt agagcgtgtc ggcgccggcg
     1621 cgtccgtcga cgacgtcatt gccactgccc gcggcgatgg cgtctcgccc ttcgctgccg
     1681 acgatcgtgt cagcgccatc ggtcacctgc caggtgtacg gcgcggcgcc tggcgacgtc
     1741 gtcggatcgg caaccgcgac gccgtcgatc acgacgccgc tcgtcgcgat ccagcgatcg
     1801 ttgagcggat cggtatcgcc gaagtcctgg agcaatccct cgatgcggac cggtgcggcg
     1861 cctggcgcca gcgcgaggcc gttcccggag atgagattgc cactcagctc cgcaccgacg
     1921 acgcccgaga ggaccacgcc ttccttctgg ttgccggtga tcgcgacgcc gtcgatgacg
     1981 aggcccgtgg tctggcgcac ttcgatgccg gcgcgaccgt tgtcgtgcac cgacccgcct
     2041 gtgatcgtga catgtgctgt ccacgagcgg atctcgttgt ctccggtctg ggcgacgatc
     2101 cccgagccgc cgttgccgta ggcctcgttg ccgctcatga gcaccgccgt cgatccggtg
     2161 acgatgttga agccgtggcg gccgttggcg taggcaatgt tgttcgatag cgtgacgccc
     2221 ttcgagaagt cgatggtaaa gccgtcgacg ccgttgtgat gggcgacgga gttggtgatt
     2281 gtcaggttgt gcgtctgctc gtgcgggtcg aagccgtagc gcgagacgtt gcggatctcc
     2341 acgcggtcga gtgtgatgtt gctgtcgaac tggccgctgt tgggcttcgg gccagaatag
     2401 aagccgtcga cgtcgcccga ggagttggcg cgattgccgt cgatcgtgag gccggcgagc
     2461 acgacgtcat gtgtcgtctg gtaggtgccg tcggtgagcg tcgagcccga atctgtcctc
     2521 acgatgccgg tcaccgcacc cgagccgtcc gcgagcttga gcaccgtcgc gcctgtgccg
     2581 gcgcccgtaa gggtcgtgtt ggagccaacc cggagcgcgc cgtcagacgc cttgccggta
     2641 ccggacaccg tgaactcgcc agccgagagc gtgacaaagc cgccgacgtg ccccgcgacc
     2701 gcgtcgaggg ccgactggat ggcggcagtc tgctgagctg cgctcagcaa cacgtcgatg
     2761 gtgatcgtcg ccgtcgcgcc gcccgaataa ccataagtga tcgtcgccat tgccactact
     2821 cccggaaaga ctgtgcctgc caactgcgac tgacgtgcaa gcgcggggcc agcaccggag
     2881 cgggtcgggt gcttcagcta aggtcaggtt tgctcactgg atcgcccgtg ctaagcagcc
     2941 ggccgggcga ttgtgaaggc cagacgaagg gaacgggacg ggaccatccg atgaagcacg
     3001 tgatcgatga gctcgagcgc cggcgcgcgg ttgcccggct cggcggcggc caggcgcgga
     3061 tcgacgccca gcacaagcgc ggcaagctca cggcgcgcga gcgcatcgag ctcctgatgg
     3121 acgccggctc cttcgaggag ctggacatgt acgtcgaaca ccgctcgacc gatttcggca
     3181 tggagaagga gaagattccg g
//
LOCUS       DAORNH010000395         3187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_22897_length_3187_cov_2.335568, whole genome shotgun sequence.
ACCESSION   DAORNH010000395 DAORNH010000000
VERSION     DAORNH010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22897_length_3187_cov_2.335568"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PK264_24050"
     CDS             complement(<1..843)
                     /locus_tag="PK264_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007775316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRD78974.1"
                     /translation="MMIYLAKRLAQLVPTLFIVSIIIFLLQQLLPGDPALVMAGEERD
                     PAVLEQIRQQYRLDQPLPVQYVYWVKGVLSGNLGESMRLKEPVLTLIAQKFPVTLQLA
                     LMAIVISLVIGVGAGIISAVRKNTAVDYSVNVVALWGISTPNFWLGIMLIFIFSVHLG
                     WLPASGYVSPFEDFWQSMKTTILPAFVLGNAFAAVLMRHTRSAMLQAMTSDYVRTARA
                     KGLNERSVVLKHAMRNALTPVITLGALELGTLLSGAVLTEQVFSIPGFGKMIVDAVFN
                     RDYPV"
     gene            967..2661
                     /locus_tag="PK264_24055"
     CDS             967..2661
                     /locus_tag="PK264_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HRD78975.1"
                     /translation="MQDTRGQSAVVVDPITVSVIAHRLRAIVEEMGEAMLRTSYSQIL
                     NSSRDFSTAICDAGGRLLAQAEHGPVHVGAMSWAARSVQGRFAGDIHPGDVFLLNDPY
                     HGGNHLPDVTAFVPVFVDGVHRFWSINRAHQSDIGGATHGAYNPAATEIWQEGLRIPP
                     LRLYEKGRLRDDLLDMIALNVRHARDFRGDLAAMVGSARLAEKRLLELAAETGTASLD
                     VAIEAVLDGAERRARAIVAAWPDGVYKGEAVLDDDGHGRRDITIRATVTISGSDFHVD
                     LTESDPQSLGFVNSSHANMQSAVCMAFAYLIDPDIPKNDGSFRPIKVTAKPGTIVWAE
                     EGRPVTLCTSHCSNEIVEAIVTALEPAAPDRVMGGWGRRFRIAIQGRDPRTKKGFIWH
                     LFQARPGGGGSIGGDGWSSVGEWHSAGGIKFGSLEVAEARFPFLFRHHEFRPGSAGAG
                     KFRGGYGCDLVMDVECDGIAKGNTAGDGVRHGSRGVLGGRDSATHRYVLRSAGRPDRE
                     LLTKETGIEIRPGDTLVIASAGGGGWGRATERDPERIEADRHNGLEATPASKQPSP"
     assembly_gap    2816..2931
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2932..3140)
                     /locus_tag="PK264_24060"
     CDS             complement(<2932..3140)
                     /locus_tag="PK264_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78976.1"
                     /translation="MAASAAASSRSATPAAIPTRPAEAARGVAPVALEPSGSSFWWWL
                     FGTGSVRRANRGLDVRWDRMRERLPT"
BASE COUNT          521 a         1037 c         1037 g          476 t
ORIGIN      
        1 caccgggtag tcgcggttga acaccgcatc cacgatcatc ttgccgaagc cggggatcga
       61 gaacacctgc tcggtcagca ccgcgcccga caacagcgtg ccgagctcca gcgcgccgag
      121 cgtgatcacg ggggtgagcg cattgcgcat ggcgtgcttc aggacgacgc tgcgctcgtt
      181 gagccctttc gcgcgggctg tgcgcacgta gtcgctggtc atcgcctgca gcatcgccga
      241 gcgggtatgg cgcatgagca cggcggcgaa cgcattgccg agcacgaagg ccggcaggat
      301 cgtcgtcttc atcgactgcc agaagtcctc gaacggcgag acatatcccg aggccggcag
      361 ccagccgagg tgcaccgaga agatgaagat cagcatgatg ccgagccaga aattgggcgt
      421 cgagatgccc cagagcgcga ccacgttgac gctgtagtcg acagccgtat tcttgcgcac
      481 cgccgagatg atgcctgcgc cgaccccgat gacgagcgag atgacgatgg ccatcagcgc
      541 gagctgaagt gtcacaggaa atttctgcgc gatgagcgtc agcaccggct ccttgagccg
      601 catcgactcg ccaagattgc cgctgagcac gcccttcacc caatagacgt actggacggg
      661 caacggctga tcgagccggt actgctgccg gatctgctcg agcacggccg gatctcgctc
      721 ctcgcctgcc atcacgagcg cgggatctcc cggcagcagc tgctgcaaca ggaagatgat
      781 gatcgagacg atgaaaagcg tcggcaccag ctgcgccagg cgctttgcga gatagatcat
      841 catgcgcgtg ggctcgccgc ttccggatgt attgcaccca taatgccgcg tgggccatcc
      901 ccccgccagc ggcttcacat cgcgcctcag ccgacctact ctcgtcgtcg aacagggggg
      961 catttcatgc aggacacgcg cggccagtcg gcagttgtcg tcgaccccat cacagtctcg
     1021 gtcatcgccc atcggctccg cgccatcgtc gaggagatgg gcgaggcgat gctgcgcacg
     1081 tcctactcgc agatcctcaa ttcgagccgt gatttctcga ccgccatctg cgatgcgggc
     1141 gggcggctcc tcgcacaggc cgagcacggg cccgtgcatg tcggcgcgat gagctgggcc
     1201 gcgcgctcgg tgcagggccg ctttgccggc gatatccacc cgggcgacgt cttcctgctg
     1261 aacgacccct atcacggcgg caatcacctc cccgatgtga ccgcgttcgt gccggtcttt
     1321 gtcgacggcg tgcaccgctt ctggtcgatc aaccgggcgc atcagagcga catcggcggg
     1381 gcgacgcacg gcgcctacaa tcccgcggcg actgaaatct ggcaggaggg actgcgcatc
     1441 ccgcctctca ggctctacga gaaagggcgg cttcgcgacg acctcctgga catgatcgcg
     1501 ctcaacgtgc gtcatgcccg cgacttccgc ggcgatctcg cggccatggt cggctcggcc
     1561 cggcttgccg agaagcgtct cctcgagctc gccgccgaaa caggaacagc gagtctcgac
     1621 gtggcaatcg aagccgtcct cgacggggcc gagcgtcgcg cgcgggcgat cgtcgccgcc
     1681 tggccggacg gtgtctacaa gggcgaggct gtcctcgacg acgacggcca cggacgccgc
     1741 gacatcacga tccgcgcgac cgtcaccatc tcggggagcg acttccacgt cgatctgacc
     1801 gagagcgatc cgcagtcgct ggggttcgtc aactcctcgc acgccaacat gcaatcggcc
     1861 gtctgcatgg ccttcgccta cctgatcgac cccgacatcc cgaagaacga cggctcgttc
     1921 cgcccgatca aggtgacggc gaagcccggt acgatcgtgt gggccgagga aggccggccg
     1981 gtgacgctct gcacgagcca ctgctcgaac gagatcgtcg aggcgatcgt cacggcactc
     2041 gagccggcgg cgccggaccg cgtcatgggc ggctggggcc gtcgcttccg catcgccatc
     2101 caggggcgcg atccacggac gaagaagggc ttcatatggc atctcttcca ggcgcgcccc
     2161 ggtggtggcg gctcgatcgg cggcgacggc tggagctcgg tcggcgagtg gcattcggcg
     2221 ggcggcatca agttcggctc gctcgaggtc gccgaggcgc gtttcccctt cctattccgc
     2281 catcacgagt tccgcccggg ctcggcaggt gccggcaagt tccgcggcgg ctacggctgc
     2341 gacctcgtca tggacgtcga gtgtgatggc atcgccaagg ggaatacggc gggcgatggc
     2401 gtccgccacg gctcgcgcgg cgtgcttggc ggccgcgaca gcgccacgca tcgctacgtg
     2461 ctgcgatccg ccggccggcc agatagggaa ctgctgacca aggagaccgg gatcgagatc
     2521 aggccgggcg atacgctggt catcgcctcg gcgggtggcg gtggctgggg cagggccacc
     2581 gagcgcgatc cggagcggat cgaagcagac cggcacaatg gcctcgaggc gacgcccgct
     2641 tcgaaacagc cgagcccttg aacgtcgaag gccggcggta gccggcctcc tagctgctcg
     2701 tcggtcagga cctgggcatc aagacctgag atttggccgt ggcccggtct ccgcgcgaag
     2761 ccccgggggg ctggggggct gagagaccag agcctcgata cgacgaccgg accacnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttggcagc
     2941 cgctcacgca tccggtccca gcgcacgtcg aggccgcggt tcgcgcgccg cacgctgccg
     3001 gtcccgaaca gccaccacca gaaactgctg ccgctcggct cgagggcgac cggtgcgacg
     3061 ccgcgagcag cttcggccgg tcgcgtcggg atcgcggcgg gggtcgcgga gcgtgaggaa
     3121 gcggctgcag atgccgccat cgtgccgacg cccgtgctcg gcggtggacc ctcgtcgggt
     3181 ccatggc
//
LOCUS       DAORNH010000396         3183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_22934_length_3183_cov_3.096547, whole genome shotgun sequence.
ACCESSION   DAORNH010000396 DAORNH010000000
VERSION     DAORNH010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22934_length_3183_cov_3.096547"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="PK264_24065"
     CDS             <1..1102
                     /locus_tag="PK264_24065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HRD78977.1"
                     /translation="RIAVRNGDRVRAGDLLLALDDTQTKANLGIVVSQLTELIGRRSR
                     LEAERDNHPEIKFPAGFETQSEDAKRIASGERRLIADRRATTASQKKQLEERVGQYEL
                     EIEGLKSQRKAKGRELEIAREELQRLEEMYRKNLIPVTRVLSVQRDIIRIEGEHGQLT
                     AQLGRVGGQISETRVQILTLEQTQRSDAQKELREIEGRIAELSERKVAAEDQLRRVEI
                     RAPQSGVVHDMAVHTVGGVVGAGEMIMLIVPSEDRLMVEVKVQPQDIDQIKLGQPAML
                     RFTAFNQRTTPEVKGQVARVAADLSKDGQTGQQFFVLRIAADEQSLSALVGLQIVPGM
                     PVEAFLEGRDRTVLSYLVRPITDQFKRALRER"
     gene            complement(1243..2538)
                     /locus_tag="PK264_24070"
     CDS             complement(1243..2538)
                     /locus_tag="PK264_24070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRD78978.1"
                     /translation="MPNPTFPDHSDRCGWNAMLPAREPRAPAMGRIDAKHVVIGAGFT
                     GLAAARRLAERDPDGDIVVLEATTVGEGASGRNSGFLAPADIAGSIEAGAVLKNSERN
                     RHLAEAFDDLLQLIGQHRIACDFHLAGRIKAAATMRGEGSVRDLRKICEALAVPNRVL
                     DVDQLRARIGTSYYRLGLFTEVGHLVQPAALIRGLADALPPAVRLCEHSPVLTLRRQG
                     SKWRVQTADADLVAANVIIAANSSIKAFGYLNDRVVTIYTYAAITQRVKSSDQGCLGE
                     MPSWGLLPAHRLGTTLRKVGLDRLLVRSLYAYERGVSAAYAEAALLDRFHRRYPDLAH
                     IEFEHVWGGTTALTMNGAPYWGRLDVGLFASAGCNGAGIVKGTILGKRLADLVLGRDS
                     GADVIAAFGTANWIAPEPFRRLGFNIASALERRRAGLES"
     assembly_gap    2933..2947
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2948..>3183)
                     /locus_tag="PK264_24075"
     CDS             complement(<2948..>3183)
                     /locus_tag="PK264_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78979.1"
                     /translation="LYKLQSGPLTAVAHAGVEDAREMSEVVATDKKLLPITDATGGGI
                     FWTRGASLLTGVSGTAVDVPRVTMLASARHLAGSG"
BASE COUNT          612 a         1001 c         1043 g          512 t
ORIGIN      
        1 ccgcatcgcg gtgcgcaacg gcgatcgcgt cagagcgggt gatctcctgc tcgcgctcga
       61 cgacacgcag accaaggcga atctcggcat cgtcgtctcg cagctgacgg agttgattgg
      121 ccggcgctcg cgcctcgagg ccgagcgcga caatcatccc gagatcaagt ttccggcagg
      181 gttcgagaca cagagcgagg acgccaagcg cattgcctcg ggcgagcgcc gcctgattgc
      241 cgaccggcgt gcgacgacgg ccagccagaa gaagcagctc gaggagcgcg tcggccagta
      301 cgagctcgag atcgagggat tgaagtcgca gcgaaaggcc aagggacgcg agctcgagat
      361 cgcgcgcgag gagctgcagc gcctcgagga aatgtacagg aagaacctca ttccggtgac
      421 gcgcgtcctt tccgtgcagc gcgatatcat ccgcatcgaa ggtgagcatg ggcagctgac
      481 ggcacaactc ggccgcgtcg gcgggcagat cagcgagacg agggtgcaga ttctcaccct
      541 cgagcagacg cagcgctcgg acgcacagaa ggagctgcgc gagatcgaag ggcgaatcgc
      601 cgagctctcg gagcgcaagg tcgccgcgga ggaccagctt cgccgcgtgg aaatcagagc
      661 gccccagagc ggcgtcgtgc acgacatggc cgtgcatacc gtcggcggcg tggtcggcgc
      721 cggcgagatg atcatgttga tcgtgccgag cgaggaccgg ctgatggtcg aggtgaaggt
      781 gcagccgcag gacatcgatc agatcaagct cggccagccg gcgatgctgc gcttcaccgc
      841 cttcaaccag cgcacgacgc ccgaggtgaa ggggcaggtc gcgcgggtcg ctgccgacct
      901 ttccaaggac gggcagaccg gtcagcagtt cttcgtgctg cgcatcgccg ccgacgagca
      961 gagcctctcg gcgctcgtcg ggctgcagat cgtaccgggg atgccggtcg aggccttcct
     1021 cgaaggtcgc gaccggacgg tactctccta cctcgtgcgc cccatcaccg atcagttcaa
     1081 gcgcgcactc cgcgagcggt gaggcgcgcg aggggtgtgg tcgggtttcc atgatcagaa
     1141 acagcacaat cagcaatgac gcaagcgcgg acgaccgcgt cagctgtcgg agatgtcgcg
     1201 tcaagggatc tggtgtttgc catctcagat atcacgatcg aactatgatt ctagcccggc
     1261 tctccgccgc tcgagcgcag atgcgatatt gaatcctagt ctgcggaacg gctccggtgc
     1321 gatccagttg gccgttccga aggcggcgat cacgtcggca cctgaatccc gtcccagcac
     1381 cagatcggca agccgcttgc cgagaatcgt gcccttgacg atgcccgcac cattgcagcc
     1441 ggcggacgcg aacagaccga catcgagccg tccccagtat ggggcgccgt tcatggtcag
     1501 tgctgttgtt ccgccccata cgtgctcgaa ctcgatatgg gcaagatcag ggtaacgacg
     1561 gtggaagcga tcgagcagcg cggcctcggc atatgcagcg gacacgccac gctcgtacgc
     1621 gtaaagtgat ctcaccagca gccgatccag gccgaccttg cgcagagtgg tgccgagacg
     1681 atgtgcgggc aataatcccc atgacggcat ttcgccgagg cagccctggt ccgaggactt
     1741 aacgcgttgg gtgatagcag cataggtgta gatggtaacg acacgatcgt tcagatagcc
     1801 gaacgccttg atcgacgaat ttgcagcgat gatcacattg gccgcgacta gatctgcatc
     1861 tgccgtctga actcgccact tgctgccctg gcgccgcagc gtgagaaccg gcgaatgctc
     1921 acaaaggcgt actgcaggtg ggagcgcgtc ggcgaggccg cggatcaggg cagcgggctg
     1981 tacgagatga ccgacttcgg tgaacagccc gaggcgatag taactcgttc cgatccgcgc
     2041 acgcagctgg tccacgtcga gaacgcgatt gggcaccgcc aatgcctcgc agatctttcg
     2101 cagatcccgc acagagcctt cgccgcgcat cgtcgcagct gccttgatcc ggcccgccag
     2161 gtggaaatca caagcgattc gatgctgtcc gatcagttga agcaggtcat cgaatgcctc
     2221 ggccagatgc cggttgcgtt cactgttctt cagaacagca ccggcttcga tgctgcccgc
     2281 gatgtcagcg ggcgccagaa agccggaatt gcgacctgac gccccctcgc cgactgtcgt
     2341 cgcttcgagc acgacaatgt cgccatccgg gtcgcgctcg gccaagcgtc gtgccgcggc
     2401 aagcccggtg aaacccgcac cgattacgac gtgcttggca tcaatgcgac ccatggcagg
     2461 agcacgcggc tcccgagcgg gcaacatggc gttccagccg cagcgatccg aatgatccgg
     2521 aaaggtcggg ttaggcatgc tgtccctgtc gagctactcg tgacgccccg gctaaggtct
     2581 tgctgtgcag gggcggagtt ggagccatcg aaaacatgac aacccggagc cgtctaaaca
     2641 cggcggttcc tgtttgcgcc gatggccgag gctgctgggt aagccggcat gatcggctgc
     2701 gcatccgtcg acgtgacgca tctgctacac atactccagg ccgcggttgg tcgatcgcgt
     2761 cacaaccatt caagcgcgca ctccgcgagc gttgaggcac gcccccccgc cgcccggtca
     2821 gcggccttcg cgccaccagg cgagtgcgat caggaagatc agcgcggaga gtgcgagcag
     2881 gcccgtgaac atgggcgtga gcttcacgcc gcgcgtcacg taggcctcgc gannnnnnnn
     2941 nnnnnnnccg gagccggcga ggtggcgggc gcttgccagc atggtgactc gggggacatc
     3001 gaccgccgtg cctgagacgc ccgtgaggag gctcgcgccg cgcgtccaga agatgccgcc
     3061 gcccgtcgca tccgtgatcg gcaacagctt cttgtccgtg gcgacgacct ccgacatctc
     3121 gcgggcgtcc tcgacgccgg catgcgcgac ggcggtgagc ggccccgact gcagcttgta
     3181 gag
//
LOCUS       DAORNH010000397         3172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_23050_length_3172_cov_2.775425, whole genome shotgun sequence.
ACCESSION   DAORNH010000397 DAORNH010000000
VERSION     DAORNH010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23050_length_3172_cov_2.775425"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK264_24080"
     CDS             <1..199
                     /locus_tag="PK264_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78980.1"
                     /translation="HADGATAAEEALRLLLPYAERLPAAHGPLAGTIAKDIIVYSEKA
                     GREPDVTLLTRLAIILGSKGS"
     gene            complement(396..2648)
                     /gene="parC"
                     /locus_tag="PK264_24085"
     CDS             complement(396..2648)
                     /gene="parC"
                     /locus_tag="PK264_24085"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HRD78981.1"
                     /translation="MSDKPTTGSQGPIEPVRLRDALEERYLAYALSTIMHRALPDVRD
                     GLKPVHRRILYAMRELRLDPTGGYKKCAKIVGEVMGNFHPHGDQAIYDSLVRLAQSFA
                     VRYPMVDGQGNFGNIDGDNPAAMRYTESRLTTVAEALLDGIDENTVDFRATYDGSNQE
                     PVVLPANFPNLLANGAAGIAVGMEQNIPPHNVDELCQALLHLIKFPNATVEKLVDLIP
                     APDFPTGGIIVEPREQIIEAYRTGRGGFRLRARWEKEDAGRGTWQIVVTEIPYQVQKA
                     RLVEKIAELIQEKKLPLVGDVRDESAEEVRLVLEPKSRTVDPTVMMESLFKLTELETR
                     FGLNMNVLSAGQIPAVLGLREVLKMWLEHRQEVLVRRSEFRLGKIEHRLEVLDGYLIA
                     FLNIDEVIRIVRFEDEPKAKLISTFKLTEVQAEAILNLRLKSLSRLEEMEIRGEHDKL
                     SKERKGLKELLASEDKQWEKISGEIKETRERWGKKTEIGRRRSELAEAPAIEVDLATA
                     ALIEKEPVTVILSEKGWIRAMKGHLDDLARVEFKQGDGLKRAIKAQTTDKLLVFATNG
                     KFFTLEASTLPGGRGHGEPVRLMVDLEENHDIVELFVYEPGRKLLVASTGGNGFIVPE
                     DEVVASTRKGKQVLNLTEPEEARLCVPAEGDTVATIGENRKMLIFPLAEVNEMTRGKG
                     VILQRFKDGALSDARVFTERDGLAWIDAAGRNFTLPKSDYKEWIGQRAQAGKLAPKGF
                     PKSNKFGQGF"
     gene            2845..>3172
                     /locus_tag="PK264_24090"
     CDS             2845..>3172
                     /locus_tag="PK264_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78982.1"
                     /translation="MLIWPPEQRTAAGGGVITGVGWLLRHWPPTKFGAFGGHRHWPPK
                     LTCPPEQVTGVTHTPPRSNLPEGHEQPVPAEFSTWPPKQVIWAAHTPFLSVLPVGHVQ
                     PVPAEFR"
BASE COUNT          465 a         1033 c         1084 g          590 t
ORIGIN      
        1 ccatgccgac ggagcaacgg ccgctgagga ggcgctgcgc ctgctgttgc cctacgccga
       61 acggctgccc gccgcgcacg gtccgctcgc aggcacaatc gccaaggaca tcatcgtcta
      121 tagtgagaag gccggtcgcg agccggacgt gacgctgctc actcgcctcg ccatcattct
      181 cggctccaag gggtcctgac ggatcggtgt ttcggtgtca gacccatggg tccgataccg
      241 gccaaccgaa gttttgactc gagggcttcc cagcggttgg ccggtatcac tcttttcgtg
      301 cgcgcggccg ccccgccaac cccgcgcgcg atactacgca acgaaaggtc aatcctttcg
      361 ttgctccgta tgacaccgtc tgcccccttc tgccctcaaa acccctggcc gaacttgttg
      421 gatttgggaa agcctttggg ggcgagcttg ccggcttggg cgcgttggcc gatccactcc
      481 ttgtagtcgg acttcggtag cgtgaagttg cgccccgcgg cgtcgatcca ggcaaggccg
      541 tcgcgctcgg tgaagacgcg cgcgtcggag agtgcgccgt ccttgaagcg ctgcaggatc
      601 acgcccttgc cgcgcgtcat ctcgttcacc tcggcgagcg ggaagatcag catcttgcgg
      661 ttctcgccaa tggtggcgac ggtgtcgccc tccgccggca cgcagaggcg cgcctcctcg
      721 ggttcggtga gattgagcac ctgcttgccc ttgcgcgtcg aggcgaccac ctcgtcctcc
      781 ggcacgatga agccgttgcc gcccgtcgaa gcgacgagca gcttgcggcc gggctcgtag
      841 acgaacagct cgacgatgtc gtggttctcc tcgaggtcga ccatcagacg cacgggctcg
      901 ccgtggccgc gcccgccggg aagcgtgctc gcttcgagcg tgaagaattt gccgttggtc
      961 gcgaacacga ggagcttgtc ggtcgtctgc gccttgatgg cgcgcttcag gccgtcaccc
     1021 tgcttgaact cgacgcgggc gaggtcgtcg agatggcctt tcatcgcccg gatccagccc
     1081 ttctcggaga ggatcacggt gaccggctcc ttctcgatca gcgccgcggt cgcgagatcg
     1141 acctcgatcg cgggcgcctc ggcgagctcg gagcggcggc ggccgatctc ggtcttcttt
     1201 ccccagcgct cgcgcgtctc cttgatctcg cccgagatct tctcccactg cttgtcctcg
     1261 gaggcgagca gctccttcaa gcccttgcgc tccttcgaga gcttgtcgtg ctcgccacgg
     1321 atttccatct cctcgaggcg ggagagcgac ttcaggcgca gattgaggat cgcctcggcc
     1381 tgcacctcgg tgagcttgaa ggtcgagatg agcttggctt tgggctcgtc ctcgaagcgg
     1441 acgatgcgga tcacctcgtc gatgttgagg aaggcgatga ggtagccgtc cagcacctcg
     1501 aggcgatgct cgatcttgcc caggcggaac tcggagcggc ggacgagcac ttcctggcgg
     1561 tgctcgagcc acatcttgag cacttcgcgc aagccgagca cggccgggat ctgccctgcc
     1621 gagagcacgt tcatgttgag gccgaagcgc gtctcgagct cggtcagctt gaagaggctc
     1681 tccatcatca cggtcgggtc gacagtgcgg ctcttcggct cgaggacgag gcggacctcc
     1741 tcggcggatt cgtcgcgcac gtcgccgaca agcggcagct tcttctcctg gatgagctcg
     1801 gcgatcttct cgacgaggcg cgctttctgc acctgatagg ggatctcggt gacgacgatc
     1861 tgccaggtgc cgcggcccgc gtcctccttc tcccagcgcg cgcggaggcg aaagccgccg
     1921 cggcccgtgc ggtaggcctc gatgatctgc tcgcgcggct cgacgatgat gccgcctgtc
     1981 gggaaatccg gcgcggggat caagtcgacg agcttctcga ctgtcgcgtt cgggaacttg
     2041 atgaggtgga ggagcgcctg gcagagctcg tccacgttgt ggggcgggat gttttgctcc
     2101 atgccgacgg cgatgccggc ggcgccgttg gcgaggagat tggggaagtt cgccggcagc
     2161 accaccggct cctgattgga gccgtcgtaa gtcgcccgga agtcgaccgt gttctcgtcg
     2221 atgccgtcga gcagcgcttc ggcgacggtg gtgaggcggc tttccgtgta gcgcatcgcg
     2281 gcggggttat cgccgtcgat gttgccgaag ttgccctggc cgtcgaccat cgggtagcgg
     2341 acggcgaagc tctgcgcgag gcgcacgagc gaatcgtaga tcgcctggtc gccgtgcggg
     2401 tggaagttgc ccatcacctc gccgacgatc ttggcgcact tcttgtagcc gccggtcggg
     2461 tcgaggcgga gctcgcgcat ggcgtagagg atgcgccggt gcacgggctt caagccgtcg
     2521 cgcacgtcgg ggagagcccg gtgcatgatg gtcgagaggg cataggcgag atagcgctcc
     2581 tcgagcgcgt cgcgcagcct gacgggctcg attggcccct gggacccagt cgtcggttta
     2641 tcgctcatgc gcccgggtta gccgagcaac ccgggcttct caagggggac gagcgcgctt
     2701 gtcccccgcg ttggtgtcgc tctgcctcag cggacgttca gaccgcgtgg cggcggcggc
     2761 ctgacggccg gcgccggctg gttgccccgg cactgaccac cgacgaagtt cggcgtgctc
     2821 ggcgggcaac ggcaggcgcc gccgatgttg atctggccac cagagcaacg cacggccgcc
     2881 ggcggcggcg tgatcaccgg tgtcggctgg ttgctccggc actggccgcc gacgaagttc
     2941 ggcgcgttcg gcgggcaccg gcactggccg cccaagttga cttgtccgcc cgagcaagtg
     3001 accggggtaa cgcacacgcc tccgcgcagc aatttgccgg aggggcacga gcagcccgtg
     3061 ccggcggagt tcagcacttg gccgccaaag caggtgatct gggcggcgca cacgccgttc
     3121 ctgagcgtct tgcccgtcgg gcacgtgcag cccgttccag cggagttcag ga
//
LOCUS       DAORNH010000398         3161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_23144_length_3161_cov_2.918545, whole genome shotgun sequence.
ACCESSION   DAORNH010000398 DAORNH010000000
VERSION     DAORNH010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23144_length_3161_cov_2.918545"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="PK264_24095"
     CDS             <1..1160
                     /locus_tag="PK264_24095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRD78983.1"
                     /translation="IGEKVKRIEALAVEIAKHVPGADEAKVRRAAQLAKADLVSGMVG
                     EFPELQGLMGRYYAEKAGTKPEIARAIELHYKPKGPSDTVPREEAGDAVAVAVALADK
                     LDTLVGFWAIGEKPTGSGDPYQLRRAALGVIRIVLENDLRLPLFRRVRNHALAYIESM
                     ETDRFLRAVKAVENLPPEAREAKRVAESEIVLASVFGVSWATKFEKAVTVSDDLLAFF
                     ADRLKVHLREKGARHDLIDAVFSLGGQDDLALIVRRVEALAEFLATDDGANLLAGAKR
                     AANILAIEEKKDKRTYDGAPNVALFAAPEEKALAAAIDKVSTDTRKAIDVENFAGAMR
                     ALAELRAPVDAFFEKVTVNVDDKAVRENRLKLLARIRAATLAVADVSKIAG"
     gene            1164..2138
                     /locus_tag="PK264_24100"
     CDS             1164..2138
                     /locus_tag="PK264_24100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78984.1"
                     /translation="MLPIAVRLRATLALLLVALAAVAVLRPAPAAAQTDPARLALDIE
                     QLARSLVGGNETVIMLNARHDPQDRGLADPLVVDMLDAIEAALSRIGHIRIVDPSAFG
                     VSLNQVWDLWGRDRGKLTAELWKSETTRVNIILMLERRGDRRARFVVYSPVSHNAKYT
                     ETLSLERPVAPTPMAPAKPVAPAAPTPGPIARPVPPLAASGPPCDTALAFLHDSGVFG
                     SRPLGDRNYAPTVDYSGSLNVPREQIVKRIREFEQRWPSRTYDIDKATVRAGALDAKR
                     CSVQFEYDFVLQRGERRTSGRARARFVLTWSSDGFLVSSEATETVSGR"
     gene            complement(2152..>3161)
                     /locus_tag="PK264_24105"
     CDS             complement(2152..>3161)
                     /locus_tag="PK264_24105"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HRD78985.1"
                     /translation="PNDHVNMSQSSNDTYPTAMHIAAAEQVVHDLLPALRKLHEALLV
                     KSHAWKDIIKTGRTHTQDATPVTLGQVFSGYATQVANGILRIEQTLPMLMELAQGGTA
                     VGTGLASPRGFAEKVAERIAAITGLPFTSAPNKFEALAAHDAMVMTHGAITAVAMGCF
                     KIANDIRLLGSGPRSGLGELALPENEPGSSIMPGKVNPTQSEALTQVAVQIHGNNAAM
                     SFAGSQGHFELNVYNPVMAYNFLQSVRLLADAAVSFTDNCVVGIEPRLDNIRRGLENS
                     LMLVTPLKEKYGYDLAAKIAKTAHKNGTTLREEAVKAGVPAADFDAIVRPEKMIGPDP
                     A"
BASE COUNT          506 a         1041 c         1080 g          534 t
ORIGIN      
        1 ccatcggcga gaaggtgaag cgcatcgagg cgctggcggt cgagatcgcc aagcacgtgc
       61 ccggcgccga cgaagcgaaa gtgcgccgcg cggcgcagct cgccaaggcc gatctcgtct
      121 cggggatggt cggagagttc ccggagctgc agggcctcat gggccgctac tacgccgaga
      181 aggccggcac caagcccgag atcgcccgcg cgatcgagct tcattacaag ccgaagggcc
      241 caagcgacac ggtgccccgc gaggaagcgg gcgacgccgt cgccgtcgcc gtggcgctcg
      301 ccgacaagct cgatacgctc gtcggattct gggcgatcgg cgagaagccg acgggctcgg
      361 gcgatcccta tcagctccgc cgcgcggcgc tcggcgtgat ccgcatcgtg ctcgagaatg
      421 atttgaggct gccgctgttc aggcgagttc gcaatcacgc gttggcctac atagagtcga
      481 tggagacaga ccgctttctt cgtgcggtta aggcggttga gaacctgcct ccagaggcac
      541 gagaggcgaa gcgggttgcc gaatcagaga tcgtcttagc ttcggttttc ggggtttctt
      601 gggcgacaaa attcgagaag gcggtaactg tatcagacga cctgctcgcc ttcttcgccg
      661 accgcttgaa ggtgcacttg cgcgagaagg gcgcccgcca cgacctgatc gacgccgtgt
      721 tctcgctcgg cgggcaggac gacctggccc tcatcgtgcg ccgcgtcgaa gcgctcgcgg
      781 agttcctcgc gacggacgac ggagcgaacc tgctcgccgg tgccaagcgt gccgcgaaca
      841 tcctcgcgat cgaggagaag aaggacaagc gcacctacga cggcgcgccg aacgtcgcgc
      901 tgttcgcggc gcccgaggag aaggcgctcg ccgccgcgat cgacaaggtc tcgacagaca
      961 cgcggaaggc gatcgacgtc gagaatttcg cgggcgccat gcgggcgctc gcggagctgc
     1021 gcgcgccggt cgatgccttc ttcgagaagg tgacggtgaa cgtcgacgac aaggcggtgc
     1081 gcgagaaccg cttgaagctg ctcgcccgca tccgcgccgc gacgctcgcc gtcgccgacg
     1141 tctcgaagat cgcggggtga gagatgttgc cgattgctgt tcgactgcgg gcgacgctcg
     1201 cgctgctcct ggtcgcgctt gcagccgttg ccgtgctccg cccggcgccc gccgccgccc
     1261 agacggaccc cgcccgcctc gccctcgaca tcgagcagct cgcccgctcg ctcgtcggcg
     1321 gcaacgagac ggtgatcatg ctgaatgcac ggcacgatcc gcaggaccgc ggcctcgccg
     1381 atccgctcgt cgtcgacatg ctcgacgcca ttgaggccgc attgagccgc atcggtcaca
     1441 tccgcatcgt ggacccttcg gcgttcggcg tgtctctcaa tcaggtctgg gacctttggg
     1501 gccgcgaccg cggtaagctc acggcagaac tctggaaaag cgagacgacg cgcgtcaaca
     1561 tcatcctgat gctcgagcgg cggggcgatc ggcgcgcgcg tttcgtcgtc tacagccctg
     1621 tctcgcacaa cgcgaagtac accgagacgc tgagcctcga gcggcccgtc gcgccgacgc
     1681 cgatggcacc tgcgaaaccc gttgctccag cggcaccgac acccggcccg atcgcgcggc
     1741 cagtaccgcc cctcgctgcc tcggggccgc cctgcgatac ggcactcgct ttcctgcacg
     1801 actccggcgt ttttggctcg cggcccttgg gcgaccgcaa ctacgcgccg accgtcgact
     1861 attccggatc gctcaacgtg ccgcgagagc aaatcgtgaa gcgcatccgc gagttcgagc
     1921 agcgctggcc ctcgcgcacc tacgatatcg acaaggcgac cgtgcgcgcg ggggcgctcg
     1981 atgccaagcg ctgctcggtg cagttcgagt acgatttcgt gttgcagcgt ggtgaaaggc
     2041 gcacgtcggg ccgagcccgc gcgcgcttcg tcctcacctg gagctctgac ggcttcctcg
     2101 tctcgtccga ggcaacggag acggtcagcg gccgctgaga acccgttcag cttatgccgg
     2161 atcggggccg atcatctttt cgggccgcac gatcgcgtcg aagtcggcgg cgggcacgcc
     2221 ggccttcacg gcctcctcgc gcagggtcgt gccgttcttg tgcgcggtct tggcgatctt
     2281 ggcggcgagg tcgtagccgt acttctcctt gagtggcgtc accagcatca gcgagttctc
     2341 gaggccgcgg cggatgttgt cgaggcgcgg ctcgatgccg acgacgcaat tgtcggtgaa
     2401 cgacacggcg gcatcggcga gcaggcgcac cgactgcagg aaattgtagg ccatcactgg
     2461 attgtagacg ttgagctcga agtggccctg gctgccggcg aagctcatcg ccgcgttgtt
     2521 gccgtgaatc tggacagcga cctgggtgag cgcctccgac tgggtcggat tgaccttccc
     2581 cggcatgata gaggagccgg gctcgttctc tggcaaggcc agctcgccga ggcccgaacg
     2641 cggtcccgag cccaacagcc ggatgtcgtt ggcgatcttg aagcagccca tggcgacggc
     2701 cgtgatggcg ccgtgcgtca tcaccatggc gtcgtgggcg gcgagcgcct cgaacttgtt
     2761 gggagccgaa gtgaagggga ggccggtgat ggccgcgatg cgctcggcga ccttctcggc
     2821 aaagccccga ggcgaggcga ggccggtgcc gaccgccgtg ccgccctgcg cgagctccat
     2881 cagcatcggc aacgtctgct cgatgcgcag gatgccgttg gcgacctgcg tggcatagcc
     2941 gctgaacacc tggccgagtg tcacgggcgt cgcgtcctgc gtgtgggtgc ggcccgtctt
     3001 gatgatgtcc ttccaggcgt ggctcttgac gaggagcgcc tcgtgcagct tgcgcagcgc
     3061 cggcaggaga tcgtgcacca cctgctcggc cgccgcgatg tgcatcgccg tcggataggt
     3121 gtcgttcgac gactggctca tgttgacgtg gtcgttcggg t
//
LOCUS       DAORNH010000399         3136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_23406_length_3136_cov_2.071081, whole genome shotgun sequence.
ACCESSION   DAORNH010000399 DAORNH010000000
VERSION     DAORNH010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23406_length_3136_cov_2.071081"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PK264_24110"
     CDS             <1..512
                     /locus_tag="PK264_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRD78986.1"
                     /translation="GAQTVTNIVAKAHEVGIDVRRDDVRFILEVVSEADPWFEQGASA
                     NLFAGRFRNFVVGRCRSQGLNLSADELDLVEAWFAGSGKDPVTAGLAARSQPAPAPAV
                     PVTAPRAAPQTERSWSLEDGRQHVAEQRYPAPTAPRAPAAPAAPAPAPAAELGNDEFP
                     RIVRTRLRG"
     gene            complement(597..1676)
                     /locus_tag="PK264_24115"
     CDS             complement(597..1676)
                     /locus_tag="PK264_24115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HRD78987.1"
                     /translation="MHFGQFNLMGYRTPGTDLGALYDSAIAQAKAAEANGFEISWFAE
                     HHFSNYCVCPSPLMMVARLAGETSRIKLGTGVVVAPLYNPLRLVSEIGMVDAMCGGRL
                     VLGVGSGYQPFEFERFGEELADAPGKLVEILEMLELAVSQESFAYAGRHYAVPTTHVA
                     TRPKRGMPDIWLAGDNAELHRLAARKGYTVMVTPRHFSADLLASARSKFEATYRSAGA
                     DPRGLKFAALRHMCVTDSKAEAADFLDNVRYQIRLSQSLRLEFQEIEGGMLTEKTYPN
                     EAPIEDMARHMLIGDPETIAERMVAEIRRAAPCHYLLHFQAGASSLRTALSSIERFAG
                     EVRPLVEKAVGPLERIGVPSVAVAE"
     gene            1933..2027
                     /gene="ffs"
                     /locus_tag="PK264_24120"
     ncRNA           1933..2027
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK264_24120"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2201..3136
                     /gene="dnaX"
                     /locus_tag="PK264_24125"
                     /pseudo
     CDS             2201..3136
                     /gene="dnaX"
                     /locus_tag="PK264_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
BASE COUNT          517 a         1103 c         1026 g          490 t
ORIGIN      
        1 ccggcgccca gactgtgacg aacatcgttg ccaaggccca cgaggtcggc atcgatgtgc
       61 ggcgcgacga cgtgcgattc atcctcgagg tggtgagcga ggccgacccc tggttcgagc
      121 agggcgcatc ggccaatctc ttcgccggcc gcttccgcaa cttcgtcgtc ggacgctgcc
      181 gcagccaggg gctcaacctg tccgccgacg agctcgatct cgtcgaggcc tggttcgcag
      241 gcagcggcaa ggatccggtc accgccggcc tcgctgcccg gagccagcct gcgccggcac
      301 ctgccgtacc tgtgacggct cctcgtgcgg ccccgcagac cgagaggtcg tggtcgctcg
      361 aggacgggcg ccagcatgtc gccgagcaac gctatccggc ccctaccgcg ccacgtgcgc
      421 ccgcggctcc ggctgctcca gcacctgctc cggcagccga gctcggcaac gacgagttcc
      481 cgcgtatcgt gcgcacgcgc ctcaggggct gaggcgcata gtcgagcgtc agtgaccgca
      541 acgggcggca ggagcattcc ggccgcctcg attgcgtttc acctgacgcg atcgtctcac
      601 tcggccaccg ccaccgacgg cacgccgatc cgctcgagcg gcccgaccgc cttttcgacg
      661 agggggcgta cctcgcccgc aaagcgctcg atcgagctga gcgccgtcct gagactcgaa
      721 gcccccgcct ggaagtgcag cagatagtgg cacggcgcag cgcgcctgat ttcagccacc
      781 atgcgctcgg caatggtctc gggatcaccg atcagcatgt gccgcgccat gtcctcgatc
      841 ggtgcctcgt tgggatacgt cttctcggtc agcatgccgc cctcgatctc ctggaactcg
      901 aggcgcaggc tctgcgagag gcggatctgg taacgcacgt tgtcgaggaa atcggcggct
      961 tccgccttgc tgtcggtcac acacatgtgg cggagcgcgg cgaacttgag gcctcgcgga
     1021 tcggcgcccg ccgaccggta cgtcgcctcg aacttcgatc gggcggacgc caggaggtcg
     1081 gccgagaagt gccgtggtgt caccatcacc gtgtagccct tgcgcgcggc gagcctgtgc
     1141 agctcggcat tgtcgccggc gagccagatg tccggcatgc cgcgcttcgg ccgcgtcgcg
     1201 acgtgcgtcg taggaacggc gtagtgcctc cccgcgtagg caaagctctc ctgcgacact
     1261 gcaagttcga gcatttcgag gatctcgacg agcttccccg gcgcgtcggc cagctcctcc
     1321 ccgaagcgct cgaactcgaa gggctggtag ccgctgccga caccgagcac gagccgcccg
     1381 ccgcacatgg catcgaccat gccgatctcc gagacgaggc gcagcgggtt gtagaggggt
     1441 gccacgacga cgccggtgcc gagcttgatg cgcgacgtct cgccggcgag ccgcgctacc
     1501 atcatcagcg gcgaggggca gacacaatag ttcgagaagt ggtgctcggc gaaccagctg
     1561 atctcgaagc cgttcgcctc tgccgctttc gcctgcgcga tggcactgtc gtagagcgcg
     1621 cccagatcgg tgccgggcgt gcggtagccc atgagattga actggccgaa gtgcatgtcc
     1681 gtgctcgcga tggatgttcc tcgccggatg atagcctgct ttcgatcgat tgcacgcgct
     1741 ccgctcggac cgacatcctc cgaagctccc gttctcatga cacagtccaa aaaatgacag
     1801 ggttcgggga gccgagcccc cgagcaggtc acagggcggc agccctgatc gcccagcgaa
     1861 cgccaactat cgccgcgcga aaatcacgcg aatgggttgt catccaccgc cccccgttgc
     1921 ataagaccgg cgggaggctg gtggcgggcg tatccgtcgc caaccgggtc aggtccggaa
     1981 ggaagcagcc ctaacgatca ggattcgggt cgtcgccagt ctctcaagtt tccctgtcac
     2041 ggccggccga gtgcaaccgg catccgcgcc cggacccgag cgacttggcg agcagcgcgg
     2101 cgagatcgtg gcacgcaaga aggcatcaga cccggcagac gtcgcggcgc cggcagcagc
     2161 gccacccgcg agtgcggagg cgccgcggcc cgcctacgtc gtgctcgcgc gcaagtaccg
     2221 cccccagacc ttcgacgacc tgatcggcca ggaggcgatg gtccagacgc tgcgcaacgc
     2281 atttgcctcg gagcgcatcg cgcagggcta catgctgacc ggcgtgcgcg gcgtcggcaa
     2341 gacgaccacg gcccgcatcc tcgcccgcgc gctgaatttc gtgctccccg gcaaagtcga
     2401 tcggccgcgc gctcaactat gagaccgacg aggtccatgc gccgacggtc gatatgccgg
     2461 tgctcggccg ccattgcgcc gagatcatgg agagccgcca ccccgacgtg ctcgagatgg
     2521 atgcggcctc caacaccggc atcgacaaca tccgcgagat catcgagagc gcgcgctaca
     2581 agccgatctc tgcccgcacc aaggtgttca tcatcgacga ggtgcacatg ctgtcgaagg
     2641 gcgcattcaa cgcccttctg aagacgctcg aggagccgcc cgggcacgtg aagttcatct
     2701 tcgcgacgac ggaggtgcgc aaggtcccgg tgaccgtgct ctcgcgctgc caacgcttcg
     2761 acctgcgccg cgtcgatgtc gatgtgcttg cgcgccactt cgagcgcatc gtcaccgcgg
     2821 agggggcgaa gtcatcccct gacgcactcg ccctcatcgc gcgcgccgcc gaaggctccg
     2881 tccgcgacgg cctctcgatc ctcgaccagg cgatcgccat gggctctggc gacgtgaccg
     2941 tcgcgagcgt gcgctcgatg ctcggcctcg ccgaccgctc gcgcattttc gatctcgccg
     3001 aaaagatcgc cgcgggcgac gctgctgccg ccctgacact gctcggcgcc ctgcatcgcg
     3061 acggcgccga tccggcggag acgctggccg atctggccga ggcgcgggcg aaggcgctgg
     3121 ggggcgatgc cgagaa
//
LOCUS       DAORNH010000400         3109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_23668_length_3109_cov_2.351015, whole genome shotgun sequence.
ACCESSION   DAORNH010000400 DAORNH010000000
VERSION     DAORNH010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23668_length_3109_cov_2.351015"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..>508
                     /locus_tag="PK264_24130"
     CDS             344..>508
                     /locus_tag="PK264_24130"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRD78988.1"
                     /translation="MRPDRSFQGLILTLQRYFADYGCAILQPYDVEMGAGTFHPATTL
                     RALGPRPWKAA"
     assembly_gap    509..580
                     /estimated_length=72
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <581..1242
                     /locus_tag="PK264_24135"
     CDS             <581..1242
                     /locus_tag="PK264_24135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HRD78989.1"
                     /translation="PSPPDLQELYLGSLAAIGLDMRLHDVRFVEDDWESPTLGAWGLG
                     WEVWCNGMEVTQFTYFQQVGGYDCNPVSGEITYGLERLAMYVQGVDRVFDLNFNGRTD
                     ARKLTYGDVFLQAEQEYSRYNFEHANTEALLAHFKDAEAECKALLDKGKAGASDNDRR
                     HKLALPAYDQCIKASHLFNLLDARGVISVTERQSYILRVRELAKACCGAWLATEGGGA
                     T"
     gene            1296..1424
                     /locus_tag="PK264_24140"
     CDS             1296..1424
                     /locus_tag="PK264_24140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78990.1"
                     /translation="MTAAKADKYAKKKPFEPSPRQKRRIAEKLRKAKRRRRNARHG"
     gene            complement(1506..1721)
                     /locus_tag="PK264_24145"
     CDS             complement(1506..1721)
                     /locus_tag="PK264_24145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78991.1"
                     /translation="MSFLASFDERVRDLGFEPLALTATHLVRGALIDNAHMDPFDRML
                     AGQALSKGMSVISADQAIAALGVPVVW"
     gene            complement(1889..2023)
                     /locus_tag="PK264_24150"
     CDS             complement(1889..2023)
                     /locus_tag="PK264_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78992.1"
                     /translation="MAKRLAGRGSLEGRFPVPPDDVLIGPMSDEDLLEAFGDAAELFK
                     "
     gene            complement(2056..2163)
                     /locus_tag="PK264_24155"
                     /pseudo
     CDS             complement(2056..2163)
                     /locus_tag="PK264_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
     gene            complement(2236..>3109)
                     /locus_tag="PK264_24160"
     CDS             complement(2236..>3109)
                     /locus_tag="PK264_24160"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRD78993.1"
                     /translation="GHHQEQADGVEHRRCTLAKPRHLDNRTLEPCGMDGGGKPDIKPA
                     APPIVSFSGKEAVGSIIVDTGARKLFFVLPGNQAYEYPISVGRDGFTWSGTERISRIA
                     SWPSWTPPAEMHKRVPGLPITVSGGLINPQGARALYLGNTIYRIHGTNNERTIGRANS
                     SGCFRMSNEHVVHLATIAKVGTTVKVVQTYAAGASAPLPSLFSFFGPADDDQPKPTAK
                     AKPKRPLPKKAVTTAKAAATVPATTSASAPAADAPTAAPASVPAPPAASAAAPATSDA
                     APAAAPAQPATKGE"
BASE COUNT          539 a          981 c         1002 g          515 t
ORIGIN      
        1 gtcgagatcg ctgaccagcc agtagccggc gaggacgcag aggccaatca ccgcggcatc
       61 gagcaatgcg gatcggtagt cgctgtaggt cgatgcgacg tggatgccct tgagcatttt
      121 catcttgcgg caggatccga tctcttccat cgagaatgtc cgacattttt tcagaaccct
      181 cttgaggaat cgccaatcgc gatccgcgcc ggatacggga attccaggct gcgccaacag
      241 gatttgaggc ttcgcttgac ccgggcggcg ccccctctgt agggtcgcgc gcctcgcgag
      301 aaacgccgga aatccgccag tgaccgaccc gctgccgccc cacatgcgtc ccgaccgctc
      361 cttccagggg ctgatcctga cgctccagcg ctacttcgcc gactatggct gcgcgatcct
      421 gcagccctac gacgtggaga tgggggcggg caccttccac ccggcgacga cgctccgggc
      481 gctggggccg cggccgtgga aggccgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccgtcgcc gcccgacctg
      601 caggagctct atctcggctc gctcgccgcg atcggcctcg acatgcggct ccacgacgtc
      661 cgcttcgtcg aggacgactg ggagagcccg acgctcggcg cctgggggct cggctgggag
      721 gtatggtgca acggcatgga ggtgacccag ttcacctact tccagcaggt cggcggctac
      781 gactgcaatc ccgtctcggg cgagatcacc tatggtctgg agcgcctcgc catgtacgtt
      841 cagggcgtcg atcgcgtctt tgacctgaac ttcaacggcc gcacggacgc gcgaaagctc
      901 acctatggcg atgtcttcct gcaggccgag caggagtact cgcgctacaa tttcgagcat
      961 gccaataccg aggcactgct ggcccacttc aaggatgcgg aggcggagtg caaggcgcta
     1021 ctcgacaagg gcaaggcagg ggcgagtgac aacgaccgtc gtcacaagct cgcactgccg
     1081 gcctatgatc aatgcatcaa ggcctcgcac ctcttcaacc tgctcgatgc gcgcggcgtg
     1141 atctcggtga ccgagcggca gagctacatc ctgcgcgtgc gcgagctcgc caaggcctgc
     1201 tgcggcgcct ggctcgcgac agagggtggt ggggctacct aatcgaagcc acagtgacgc
     1261 taacggttca gtctccagca caaggatatg gactcatgac cgccgccaaa gccgacaagt
     1321 acgcaaaaaa gaagccgttc gagccgagcc cccgccagaa gcgccgcatc gccgagaagt
     1381 tgcgcaaggc caagcggcgg cgacgcaacg cgcggcacgg ttgagacgtc gggcaggtgc
     1441 tggtcgcggc gacgcagcca cgatatccag tcgcgcgcgc ccaatgcacg cgccacgcgc
     1501 gtaggtcacc acaccactgg caccccgagt gccgcgatgg cctgatcggc cgaaatcacg
     1561 ctcatgccct tgctgagggc ctggcccgcg agcatgcgat cgaaagggtc catgtgcgcg
     1621 ttgtcgatca gagcgcctcg aaccaagtgt gtggccgtga gggcgagcgg ctcgaaacca
     1681 agatcgcgga ctcgctcgtc gaagctcgcc agaaacgaca tatcgaactc cagcttgccg
     1741 agccggtgct tcaacgcgag ttcccaggcc gaaaccgagc tgaccaagaa cgagttggtt
     1801 ctgtcattca gcgccgtttc agcggctctt ggcagcagca ggcgtgactg gactcaccag
     1861 atcagcacgt gggtgtcgag cagcaggatc acttgaacag ctcggccgca tctccgaatg
     1921 cttcgagcag gtcctcgtcg ctcatggggc cgatcagcac gtcgtcaggc ggcaccggga
     1981 agcgtccttc caacgagcct cgccccgcca gccgcttggc caagatgtca tcggccttga
     2041 aggcagtgag caccgcaatg ggtcgtggcc ccgcgcgatg acgatctcct cgcccgcctc
     2101 gacgcgccgc agcagctctg aaagccgtgt ctttgcctcg tgaattgtga cacgaacgcc
     2161 cacggccgga cccagttagc taactggacc aagtatgtgc agcggcacgg ctggcgtcaa
     2221 tgcgggcgcc gcgattcatt cccccttggt cgccggctga gcaggggcgg ctgccggagc
     2281 ggcatcggag gtcgcgggcg cggcggcgct tgccgctggc ggtgccggaa ctgatgctgg
     2341 ggcggcagtg ggcgcgtccg ctgccggagc cgatgcggac gtggtcgccg gcacggttgc
     2401 agccgctttt gccgttgtca cggccttctt gggcagaggc cgcttcggct tggccttcgc
     2461 cgtcggcttc ggctgatcgt cgtcggcggg tccgaagaag ctgaacagcg agggcagcgg
     2521 cgcgctggcg ccggccgcat aggtttgcac gaccttcacg gtggtgccga ccttggcaat
     2581 ggtggccaaa tgcacgacgt gctcgttgga catccggaag cagcccgacg agttggcgcg
     2641 gccgatggtg cgttcgttat tggtgccgtg gatgcgatag atggtgttgc ccagatagag
     2701 cgcgcgcgcg ccctgcgggt tgattaatcc gcccgacacc gtaatcggca gcccggggac
     2761 gcgcttgtgc atctccgccg gcggcgtcca ggacggccac gacgcgatcc gactgatgcg
     2821 ctcggttccg gaccaggtga agccgtcgcg gccgaccgag atcggatact cgtaagcctg
     2881 attgcccggc agaacgaaga aaagcttgcg cgcgcccgta tcgacgatga tggaaccgac
     2941 ggcctccttg cccgagaacg agacgatcgg cggcgccgcc ggcttgatgt ccggcttgcc
     3001 accgccatcc atgccgcagg gttcgagggt acgattgtcg aggtgtcgtg gtttggcgag
     3061 ggtgcaacgc cggtgctcaa cgccgtcagc ctgttcctga tggtggcct
//
LOCUS       DAORNH010000401         3073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_24013_length_3073_cov_3.090126, whole genome shotgun sequence.
ACCESSION   DAORNH010000401 DAORNH010000000
VERSION     DAORNH010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24013_length_3073_cov_3.090126"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1142
                     /locus_tag="PK264_24165"
     CDS             195..1142
                     /locus_tag="PK264_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD78994.1"
                     /translation="MASLRSIDLNLLVVLDALLRERHVTRAARTVGLSQPAMSSALAR
                     RRVVFKAELLVRTATGMQPTPRAETLIEPVQQAIGQIERVLVYDQRFDAAHSERRFAL
                     RLSDLLSYLILPRLMALVAGAAPRITVDIKHLSPQQTIDALERDEIDAAVSMGLEHTG
                     TINRRVLFRDRMVAVMRRAHPLARGSLTLKRFLATRHLKVSMAPSDIRFADDVLSKSN
                     SERDVALNLPHWLLVPHILAVTDYVAVMPAAFATAAQQADLVVRELPFAAETFDWCLY
                     WHRRHDADAANRWLRECIEAASAIDHSARRSRPRSAGAR"
     gene            complement(1164..1249)
                     /locus_tag="PK264_24170"
     tRNA            complement(1164..1249)
                     /locus_tag="PK264_24170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1214..1216),aa:Leu,seq:taa)
     gene            complement(1400..>3073)
                     /gene="lepA"
                     /locus_tag="PK264_24175"
     CDS             complement(1400..>3073)
                     /gene="lepA"
                     /locus_tag="PK264_24175"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRD78995.1"
                     /translation="LDSMDIERERGITIKAQTVRLDYKANDGKTYQLNLMDTPGHVDF
                     AYEVSRCLAACEGAILVVDASQGVEAQTLANVYQAIEGNLEILPVLNKIDLPSADADR
                     VKEQIEDVIGIDASGAILASAKTGIGIKDVLEGVVTRLPPPKGDPAAPLKALLIDSWY
                     DAYLGVVVLVRIVDGTLKKGQKLKFWSTDATHQVDRVGIFRPKQEMLAELRPGEVGFF
                     TAAIKQVADARVGDTVVDEKSSVTKPLPGFKPAQPVVFCGLFPVDAADFEDLRDAMAK
                     LRLNDASFSFETESSAALGFGFRCGFLGLLHLEIITERLEREFNLDLITTAPSVIYHI
                     HMNDGTMVEMHNPADMPDVVKIDRIEEPWIKATILVPDDYLGAVLKLCQDRRGRQKEL
                     TYVGTRAMLVYELPLNEVVFDFYDRLKSVSRGYASFDYQIIGHEEGDLVKLSILVNAE
                     PVDALSIIVHKTRAESRGRAMCEKLKDLIPPHLFQIPIQAAIGAKVIARETIRALRKD
                     VLAKCYGGDITRKRKLLDKQKKGKKKMREFGKVEIPQEAFIAALKMDEN"
BASE COUNT          500 a          982 c         1010 g          581 t
ORIGIN      
        1 acgagcgcct tcacctcgcg tttcagaatc tcggtcagtt ccatgagatc gggctccatt
       61 caggtacggt tggcctatct tggcagggcg caggcaattc gcctaatcga ttgattgtat
      121 gagtccatcc atggcatgga tgagcccgcg agcatgctcg ctccatggtt ctcgggatcg
      181 gaacgggatc acgcgtggca agcctccgca gtatcgatct caatctgctc gtcgtcctcg
      241 acgcgttgct gcgcgagcgc catgtcacgc gggcggcgag aaccgtcggc ctctcccagc
      301 ccgccatgag cagcgcgctg gcgcggcggc gcgtcgtgtt caaggccgag cttctggtcc
      361 gcaccgcgac gggcatgcag ccgacgccgc gcgccgaaac tctgatcgag cctgtgcagc
      421 aggcgatcgg gcagatcgag cgtgtgctcg tgtacgatca gcgcttcgat gccgcacact
      481 ccgagcggcg cttcgccctc cgcctgtcgg acctcctgag ctacctcatc ctgccgcgcc
      541 tgatggccct ggttgccggg gccgcgccgc ggatcaccgt cgacatcaag cacctctccc
      601 cgcagcagac gatcgacgcg ctcgaacggg acgagatcga tgccgccgtc agcatgggcc
      661 tcgagcatac gggaacgatc aaccggaggg tgctcttcag ggaccggatg gtcgccgtca
      721 tgcgcagagc gcatccattg gcgcggggaa gccttactct aaagcgcttt ctggcgacgc
      781 ggcatctgaa ggtgtcgatg gcgccgtcgg acatccgctt tgccgacgat gtgctctcga
      841 aatcgaactc cgagcgtgac gtggccctca acctgcccca ctggctgctc gtgccgcaca
      901 ttctcgcagt gaccgactat gtggccgtga tgccggctgc cttcgccacg gcggcgcaac
      961 aagccgatct cgtcgtccgc gagttgccgt tcgcggcgga gacattcgac tggtgcctct
     1021 attggcatag acgccacgac gctgatgccg cgaaccgatg gttgcgcgag tgcatcgaag
     1081 cggcgtctgc catcgatcac tcggcgaggc gctcgcgacc gcgatcggca ggggcacgct
     1141 gaccggaggc gcgcggccgg tcttggtgcg gacggcggga ctcgaacccg cacggcattg
     1201 ctgcctcggg attttaagtc ccgtgtgtct accgttccac cacgtccgct cgcccgtgcc
     1261 cgggccaccc cgccatcgcg acaatgacaa ccgaccgaag ggccaaagtc tacggcaggg
     1321 catgcgcatg ggcacgcacc acgcagcgca acacggtgtt agtgccaatg ctccgggcga
     1381 gcaactccga tacgccccct cagttctcgt ccatcttgag ggcggcaatg aaggcttcct
     1441 gcgggatctc gactttgccg aactcgcgca tcttcttctt gcccttcttc tgcttgtcga
     1501 ggagcttgcg cttgcgcgtg atgtcgccgc cgtagcactt ggcgagcacg tccttccgga
     1561 gcgcgcggat cgtctcgcgg gcgatcacct tggcgccgat cgccgcctgg atcgggatct
     1621 ggaagaggtg cggcgggatt aggtccttca atttctcgca catggcgcgg ccgcggctct
     1681 ccgcgcgcgt cttgtggacg atgatggaga gtgcatcgac cggctcggcg ttgacgagga
     1741 tcgagagctt cacgaggtcg ccctcctcgt ggccgatgat ctggtagtcg aacgaggcgt
     1801 agccccgcga gacgctcttc aggcggtcgt agaagtcgaa taccacctcg ttgagaggga
     1861 gctcgtagac gagcatggcc cgcgtgccga cgtaggtgag ctccttctgg cggccgcggc
     1921 gatcctggca gagcttcagg acggcgccga ggtagtcgtc agggacgagg atcgtcgcct
     1981 tgatccaggg ctcctcgata cgatcgatct tgaccacatc cggcatgtcg gccgggttgt
     2041 gcatttcgac catcgtgccg tcgttcatgt ggatgtgata gatgacgctt ggcgccgtgg
     2101 tgatgaggtc gaggttgaac tctcgctcga gccgctcggt gatgatctcg aggtggagaa
     2161 gcccgaggaa gccgcagcgg aagccgaagc cgagcgccgc cgagctttcc gtttcgaagg
     2221 agaagctcgc gtcgttgagg cgcagcttcg ccatggcgtc gcgcaggtcc tcgaagtcgg
     2281 cggcatcgac cgggaagagc ccgcagaaca cgacgggctg ggccggcttg aagcccggca
     2341 gcggcttggt cacgcttgat ttttcgtcga ccacggtatc gccgacgcgc gcgtcggcga
     2401 cctgcttgat ggccgcggtg aagaaaccga cctcgcccgg ccgcagctcc gcgagcatct
     2461 cctgcttcgg gcggaagata ccgacgcggt ccacctgatg ggtcgcgtcc gtcgaccaga
     2521 atttcagctt ctggcccttc ttcaaggtgc catcgacgat gcgcacgagt acgacgacgc
     2581 cgaggtaggc atcataccag ctgtcgatca gcaaggcctt cagtggcgcc gccggatcgc
     2641 ccttcggcgg cgggaggcgc gtcacgacac cctcgagcac gtccttgatg ccgatgccgg
     2701 tcttggccga ggcaaggatt gcgcccgagg catcgatgcc gatcacatcc tcgatctgct
     2761 ccttgacgcg gtcggcatcg gcggacggca ggtcgatctt gttgaggacg ggcaggatct
     2821 cgaggttgcc ctcgatcgcc tgatagacgt tggcgagcgt ctgcgcctcg acgccctggc
     2881 tcgcgtcgac gaccaggatg gcgccctcgc aggcggcgag gcagcgcgag acctcgtagg
     2941 cgaagtcgac gtggccgggc gtgtccatca ggttgagctg ataggtcttg ccgtcgttgg
     3001 ccttgtagtc gaggcgcacg gtctgcgcct tgatggtgat gccgcgctcg cgctcgatgt
     3061 ccatcgagtc gag
//
LOCUS       DAORNH010000402         3009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_24734_length_3009_cov_2.580230, whole genome shotgun sequence.
ACCESSION   DAORNH010000402 DAORNH010000000
VERSION     DAORNH010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24734_length_3009_cov_2.580230"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK264_24180"
     CDS             complement(<1..652)
                     /locus_tag="PK264_24180"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HRD78996.1"
                     /translation="MQSRTTGAAALLAVVEAEQDRWFLWGPVLFGLGIGLYFSLRVEP
                     GLTMALVPLVAVLALRWALPALGLSAMLLSGLTAMAAGFAVAKARTDLVAAPTLQTRL
                     EGVRIRGRVETLEPRPGGGGRVAIAVSSIEQLAPIDTPHRVRVRLMDSTEGATPGAAI
                     TLRATLSPPGAPIMPGGYDFARAAYFAGLGAVGFARGAIEPDTSAPPPTVTMRLRAA"
     gene            complement(716..1654)
                     /locus_tag="PK264_24185"
     CDS             complement(716..1654)
                     /locus_tag="PK264_24185"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRD78997.1"
                     /translation="MSQRKRIAIIGVGAMGSVYAGLLASAQHDVWAVDVWKDHVEAIK
                     TKGLRVEGASGDRTVKMNATTNPADVKDADLVIIATKADGVAAAAKAAKAMAKPDAPI
                     LTIQNGLGSADVVAGIVGSKRIMIGVVGGFGASMKGPGHAHHNGWEFLRLGEMDGGLT
                     PRLEDVKATWESGGFKVLTFADIHKMIWEKLICNCTYSGTCALTNLTVEEVQASPSAW
                     SVASGCATEAYHVARAKGIALDFDDPVRYVREFGQKIPKARPSMLLDHMAGRPAEVDN
                     INGAIPREGSKVGVATPINSLVVALLKAKESGFKKA"
     gene            complement(1721..>3009)
                     /locus_tag="PK264_24190"
     CDS             complement(1721..>3009)
                     /locus_tag="PK264_24190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD78998.1"
                     /translation="ARRGVPQSRQIHILLAAGHRDLERIVPGFDAALEGAGAPEFDAI
                     GHWHAHSQFGWFPVRPSEIKTRLATRGLVEHVVRQSVLAHANVTLLERTRIAAIPFAD
                     GAAQGFMVRSADTDRGEERLLPADLVVDASGRQSRLPQALEASGCGATAETVIDARIG
                     YACRWYNPPPGRTFPWRFLLIRNPRLMQRVCGIALMENGQWLVNLAGYGGDYPPNDVA
                     GFEAFLASIGCDEVARALKDAEPVSDIAGYRDTVNRWRHFERLAAWPDRLLAVGDAVC
                     AFNPVYGQGMSAAAREAQMLASTLGQGTDLRMAGACRRLQKDLARFLAGPWTLATGED
                     YRFATTLGPPRPRLMRLAHWFTDRVMQAATTDSYVQDRFMRVMHMVDAPLALANPLVV
                     ARALLSPRAAPSIDGGGRVVDGPPAYQHGRSMAAPP"
BASE COUNT          492 a         1023 c         1035 g          459 t
ORIGIN      
        1 tcgcggctcg gagcctcatc gtgacggtgg gtggtggcgc ggacgtgtcc ggctcgatcg
       61 cgccgcgcgc gaagccgacc gcgccaagtc ccgcaaagta ggcggcgcgc gcgaaatcgt
      121 agccgcccgg catgatggga gcgcccggcg gcgacaacgt cgcgcgaaga gtgatcgcgg
      181 cgccgggagt ggcgccctcc gtgctgtcca tgaggcgcac gcgcacgcga tgtggcgtat
      241 cgatcggggc gagctgctcg atcgaactga cggcgatcgc gacacgtccg ccgccgccag
      301 ggcgtggttc gagcgtctcg acccggcctc tgatccgcac accttcgagg cgtgtttgca
      361 gtgtcggcgc cgcgacaaga tccgtccgtg ccttggcaac ggcgaagccg gccgccattg
      421 ccgtcaggcc ggagagcagc atcgccgaaa ggccgagcgc cggcagcgcc cagcggagcg
      481 cgagcacggc cacgaggggg acgagggcca tggtgaggcc cggctcgacg cgcagcgaga
      541 aatagagccc gatgccgagc ccgaacagca ccggtcccca gaggaaccag cggtcctgct
      601 cggcctcgac gacggccaga agtgccgctg cgcccgttgt ccggctctgc aaacgcagac
      661 tccccattgt ccgaccacga caatggggca gcttagcgcg agtgtcccgc gtacgtcagg
      721 ccttcttgaa gcccgattcc ttcgccttca gcagcgcgac gacgagtgag ttgatgggcg
      781 tcgcgacgcc gaccttggag ccctcgcgcg ggatcgcgcc gttgatgttg tcgacctcgg
      841 ccgggcgacc cgccatgtgg tcgagcagca tcgagggccg cgccttcggg atcttctggc
      901 cgaactcgcg cacgtagcgg acgggatcat cgaaatcgag cgcgatgccc ttggcgcggg
      961 caacatggta ggcctccgtc gcgcagcccg aggcgacgct ccaggcggac ggcgaggcct
     1021 gcacctcctc gaccgtgaga ttcgtgagcg cgcacgtccc cgagtacgta caattgcaga
     1081 tgagcttctc ccagatcatc ttgtgaatgt cggcgaaggt cagcaccttg aagccgcccg
     1141 actcccaggt cgccttgacg tcctcgaggc gcggcgtgag gccgccgtcc atttcgccca
     1201 agcgcaggaa ttcccagccg ttgtggtggg catggcccgg acccttcatc gaggcgccga
     1261 agccgccgac gacgccgatc atgatgcgct tcgagccgac gatgccggcg accacgtcgg
     1321 cgctgccgag gccgttctgg atggtgagga tcggcgcgtc gggtttcgcc atcgcctttg
     1381 ccgccttcgc cgccgccgcg acgccgtcgg ccttggtcgc gatgatgacg aggtcggcgt
     1441 ccttgacgtc ggccggattg gtggtcgcgt tcatcttgac ggtgcggtcg cccgaggcac
     1501 cctcgacgcg caggcccttc gtcttgatcg cctcgacatg gtccttccac acatcgacgg
     1561 cccaaacatc atgctgcgcg ctcgcgagca gtcctgcgta gacggagccc atggctccga
     1621 cgccgatgat ggcaatgcgc ttcctctggc tcatgtactt gccctttcgc ggctggttcc
     1681 cggtgttgct gcggcgattg accccacagt acccgccgcg tcaaggcgga gcagccatgc
     1741 tccggccgtg ctggtaagct ggcggcccgt ccacgacacg tccgccgcca tcgatgctcg
     1801 gcgccgcgcg cggggagagc aaggcccgtg cgacgacgag cggattggcc agcgccaagg
     1861 gcgcatcgac catgtgcatg acgcgcatga agcgatcctg gacatagctg tccgtcgtcg
     1921 cagcctgcat gacgcggtcg gtgaaccagt gggcgagccg catgaggcgc ggccgcggcg
     1981 gaccgagtgt cgtcgcaaag cggtagtcct cgcctgttgc cagcgtccag ggcccggcaa
     2041 gaaagcgcgc gagatccttc tgcagccgtc gacaggcgcc agccattctc aagtcagtgc
     2101 cctggccaag ggtcgatgcg agcatctggg cctcgcgagc ggcggccgac atgccctgac
     2161 cataaaccgg attgaaggcg cacacggcat cgccgacggc gagcagccga tcgggccagg
     2221 cagcgagtcg ctcgaagtgc cgccagcggt tcaccgtgtc gcgatagccg gcgatgtcgg
     2281 agacgggctc ggcatccttc agggcccggg caacctcatc gcagccgatg cttgcgagga
     2341 aggcctcgaa gcccgcgacg tcgttgggcg gataatcgcc gccgtagccg gcgagattga
     2401 cgagccactg cccgttctcc atcagcgcga tgccgcatac acgctgcatc agacgtggat
     2461 tgcggatcag cagaaagcgc catggaaaag tgcggcccgg cggcggattg taccagcggc
     2521 aggcataacc aatgcgggcg tcgatcacgg tctcggcggt cgcaccgcag cccgatgctt
     2581 cgagcgcctg cgggaggcgc gactggcggc cgcttgcgtc gacgacgagg tccgccggca
     2641 gcaggcgctc ctcgccgcga tccgtgtcgg cactgcgaac catgaagccc tgcgccgcac
     2701 cgtctgcgaa cgggatcgcg gcaatgcgtg tccgctccag cagcgtgacg ttggcatgcg
     2761 ccagaacgga ctggcgcacg acatgctcga cgaggccgcg cgtcgcaagc cgcgtcttga
     2821 tttccgaggg ccgcactgga aaccagccga attgcgagtg cgcgtgccaa tgcccgatgg
     2881 cgtcgaactc gggcgcgccg gcgccctcga gcgccgcatc gaacccaggg acgatccgct
     2941 cgagatcgcg atgtccggcc gccagcagga tgtggatctg ccggctctgc gggacgccgc
     3001 gccgcgccg
//
LOCUS       DAORNH010000403         2996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_24854_length_2996_cov_2.836110, whole genome shotgun sequence.
ACCESSION   DAORNH010000403 DAORNH010000000
VERSION     DAORNH010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24854_length_2996_cov_2.836110"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..796)
                     /locus_tag="PK264_24195"
                     /pseudo
     CDS             complement(26..796)
                     /locus_tag="PK264_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
     gene            1070..1742
                     /locus_tag="PK264_24200"
                     /pseudo
     CDS             1070..1742
                     /locus_tag="PK264_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419867.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
     assembly_gap    1743..1790
                     /estimated_length=48
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1791..1991
                     /locus_tag="PK264_24205"
     CDS             <1791..1991
                     /locus_tag="PK264_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HRD78999.1"
                     /translation="GAGLDVFEHEPAVNPKLLASSRVVALPHMGSATIEGRIDMGEKV
                     IINIKTFLDGHAPPDRVHPAMF"
     gene            2073..>2996
                     /locus_tag="PK264_24210"
     CDS             2073..>2996
                     /locus_tag="PK264_24210"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HRD79000.1"
                     /translation="MRRHVAVCLLLLAACLAPGVGTVAHAQDEGTANTYITPFPPGDA
                     YRIMVIGDSHAEGMLSGLVEALGTDPRLQLQRKHRAVSGLLRVDIDEELKALEADLGR
                     ERALVAIFMLGNNDRMHFRPPTGKRANLGSDEWKEEYGRRVDLVLKLLKRRNIAVYWV
                     GLPPLRRAEANEDAKMMNEIFRERAYLNGLKFVDIFTGFADEDGDYTPTGPDLSGEHR
                     TMRASDGVHFTPAGNRKLANFVEVELKRDLNVAKAARTLPLAGSEAEQKRINAKAAAP
                     PSAAPAPGSAASTAKDGGVPAPRPAFARPWSD"
BASE COUNT          502 a         1014 c          952 g          480 t
ORIGIN      
        1 gcatgtcggc gatcgagccg cctcaatact tggccagcca gtggagcgtg tgcaggtctc
       61 tgaggccgcc cttgccgtcc ttgatgttcg gctcgacccg gtagcgcgat tcgcccgtcc
      121 gtcgatgacg ctcatcgcgc tcgccgagct tcgcctcgat gaaggcggcg gtactcgcgc
      181 tttcgccgag catctcccgg tcgaaacggc gcccgagctc gccgaagagc gacgcatcac
      241 cgagaatgag gcggccatcg aggatcgcgg tgcggatcgt catgtccgcc tgcgagagct
      301 ttatgcactg atcgaccgtg cgcgtcgcgt gcccgacctt ctgcttcagg tcccagagga
      361 tgtagagcat gtattcgacc acgctctccc cccagggcgt ctgcttgtag ggcagcagga
      421 agaggagatc gatgtccgag ccgggcgcga ggaggccgcg gccgtatccg ccggtggcga
      481 tcaccgccat gcgctcggtg tcggcgggat tggtgatcgg gtagacctgc gccactgtgt
      541 aggcatagat cgtgcgaatg agttcgtcct cgagcttgga gaggcggaca gcgcagcgcc
      601 gcccgtcgcc cgtatcgatc agctcgtcct gggccgcgcg ccgctcccgc tgcaggatcg
      661 cccgcagctg ctcgacgagc ttcccccgga ccgcctgggc attttcgccc gaaagccggg
      721 caagggcggc aagctcgtcc ttgagcaact cattgtgcgg ctccgtgcgc ggcgctggtg
      781 cgctcgacac tgtcataggc gatctccagc cattctctgc agtcgagtgg gacgctagcg
      841 aaccgtcagc gccgggtcga gggggcatcc tccgacaaag tgatgaggcg gcgcccgtgg
      901 cgccttgtgt tcatccgtgg ctctgttaga caacgccatc cgcgccgggc gcgcggacca
      961 tgcatcgaca gacctggacg agaccgactt ggagacgaac atgcccgcga gcgtcaagaa
     1021 gcctctcgtc gtggtcacgc ggaaacttcc cgacgtcgtc gagacgcgaa tgcgggaact
     1081 cttcgatgcg cgtctcaacg tcgacgacaa accgttgagt caggcggagc tcgtcgccgc
     1141 ggccaagtcg gccgacgtcc tagtgccgac cgtcaccgac cgcatcgaca ggtcggtgct
     1201 cgcccagtcg ggaccgcagc tcaagctgat cgccaacttc ggcaccggtg tcgacaacat
     1261 cgacctcgat acggcacgca atcgcggcat cattgtcacc aacacccccg gcgtgctgac
     1321 ggaagacacc gccgacatga ccatggcgct gatcctcgcc gtgccgcgtc ggcttgccga
     1381 agggaccgcg ctcctcaaga gcccgaagca gggctggacc ggctggtcgc cgacctggat
     1441 gctcggccac cgcatctggg gcaagcgcct cggcatcatc ggcatgggcc gcatcggcca
     1501 ggccgtggcc cggcgcgcca aggccttcgg cctgcagatc cactatcaca atcgccggcg
     1561 cgtcgcggaa gttgtcgagc aggaactcga ggccacctat tgggagagcc tcgaccagat
     1621 gatctcccgg atggacatcg tttccgtcaa ttgcccgcac acacccgcca cgtatcattt
     1681 gctttcggcg cggcgcctga agctgctcaa gccaacggcc tatgtggtca acacggcacg
     1741 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcgccggcc
     1801 tcgatgtctt cgagcacgag cccgccgtca atccgaagct gctcgcctcg agccgcgtcg
     1861 tggcgctccc gcatatgggc tcggcgacca tcgaaggccg catcgacatg ggcgagaagg
     1921 tcatcatcaa catcaagacc ttcctcgacg gccacgcccc ccctgaccgc gtgcatccgg
     1981 cgatgttttg agggcgacac ggccttgccc cggccaaggg ttacggctaa ccgttgatcc
     2041 ccacgtggtg cgcgttggct gaggatcgat cgatgcgccg gcatgtcgcc gtctgcctcc
     2101 tcctgctcgc ggcctgcctc gcgcccggcg tgggcacggt ggcgcacgcc caggacgagg
     2161 ggaccgccaa cacgtacatc acgccgttcc cgccgggcga cgcctatcgc atcatggtga
     2221 tcggcgacag tcacgccgaa ggcatgttgt cggggctcgt cgaggcgctc ggcaccgacc
     2281 cgcggctgca gttgcagcgc aagcatcgcg ccgtctccgg ccttctgcga gtcgatatcg
     2341 acgaggagct gaaagcgctc gaggccgacc tcgggcggga gcgggccttg gtcgccatct
     2401 ttatgctcgg caacaacgac cgcatgcatt ttcgcccgcc gaccggcaag cgggcgaacc
     2461 tcggcagcga cgagtggaaa gaggaatacg gccggcgcgt cgacctcgtc ctcaagctcc
     2521 tgaagcggcg caatatagcg gtctactggg tcgggctgcc gccgctgcgt cgcgcggagg
     2581 cgaacgagga cgcgaagatg atgaacgaga tcttccgtga gcgcgcctac ctcaatggcc
     2641 tcaagttcgt cgacatcttc acgggctttg ccgacgagga cggcgactac acgccgaccg
     2701 gcccggacct ctcgggagag catcgcacca tgcgggcatc cgacggcgtg cacttcacac
     2761 ccgccggcaa tcgcaagctc gcgaacttcg tcgaggtcga gctgaagcgc gacctcaacg
     2821 tcgccaaggc ggcgcggacc ttgccgctgg ccggctcgga agccgagcaa aagcgcatca
     2881 atgcgaaagc ggcggctccg ccctcagcgg ccccggctcc gggcagcgcg gcctcgactg
     2941 ccaaggatgg cggcgtgccc gcgccgcgcc cggccttcgc acgcccctgg tcggat
//
LOCUS       DAORNH010000404         2996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_24855_length_2996_cov_2.821829, whole genome shotgun sequence.
ACCESSION   DAORNH010000404 DAORNH010000000
VERSION     DAORNH010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24855_length_2996_cov_2.821829"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2077)
                     /locus_tag="PK264_24215"
     CDS             complement(<1..2077)
                     /locus_tag="PK264_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRD79001.1"
                     /translation="MQEKVNKGGQPNYKIIGTRPVRPDGVDKVTGRARFGADMKMAGQ
                     LVGKVLRSPHPHARIRSIDTKAAAALPGVKAVVTGADFNDQPSEFVPAGEMLINYRDV
                     VRNIMAREKALFEGHPVAAVAAISDSVAKAALKLIRVDYEVLPHVIDEVEAMKPGAPI
                     LHEDLITAGVTPPPAAASNVAKRVEFTLGNADEAFKDADVIVEREFKTKPVHQGYIEP
                     HACVASVNEDGLAELWTTTQGHWIKRAHCAKLLGWDISKLRVTSSEIGGGFGGKTVVY
                     LEPVALALSKKARRPVKMVMSREEVFRASGPTSGATVWVKMGAKRDGRIVAAAADLKY
                     QAGAFQGSPVQPGAMCAFAPYDLEHVKVVGYDVVSNRPKVAAYRAPGGPISEYAVECV
                     VDELAEKIGMDPIELRLKNAAREGTKAAYGPKFGPVGLVECLEAAKASEHWKTPLKPN
                     QGRGIAAGFWFNIGGETTVSLQLNEDGTMTLMAGTPDIGGLRASLCMMAAEELGVSVD
                     SIKPIIADTSSLGYNFLTGGSRSTFSSGMATVEAARSIIKEACSRAAKLWELSPDAVT
                     FENGEVKPAGDNAGKHKPMSLKDVARAAGKTGGPIVGYSSLTAHGAAPSFGVHVADVE
                     VDPETAHTKIVRYTVIQDAGRAIHPAYVEGQFQGGAVQGIGWALNEEYIYGKDGKLQN
                     AGFLDYRIPV"
     gene            complement(2192..2671)
                     /locus_tag="PK264_24220"
     CDS             complement(2192..2671)
                     /locus_tag="PK264_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRD79002.1"
                     /translation="MSKYYVSATVNGDPVEFLCEPTQTLLDCLRDELSLTGAKEGCSS
                     GDCGACSVTVDGRLVCSCLVLAVEANGRKIETIEGMAKDDNLHPLQKKFLEHAALQCG
                     ICTPGFLVAAKALLEKNPNPTETEIRYWLAGNLCRCTGYDKIIRAVQDAAAEMRRST"
     gene            complement(2674..>2996)
                     /locus_tag="PK264_24225"
     CDS             complement(2674..>2996)
                     /locus_tag="PK264_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRD79003.1"
                     /translation="FIPRTEMDIAVVGCGVALTIDGAGKCTAARVALGAVAERPLLVD
                     AAAKALIGTPVDDAAMQALDAAARAACRPIDDKRGTKEFRIDVAGVLARRTAAIALER
                     ARKR"
BASE COUNT          454 a         1001 c         1008 g          533 t
ORIGIN      
        1 caacggggat gcggtagtcg aggaagcccg cgttttggag ctttccatcc ttgccgtaga
       61 tgtactcctc gttcagggcc cagccgatgc cctgcacggc gccgccctgg aactggcctt
      121 ccacgtaagc cgggtggatg gcgcggcccg cgtcctggat cacggtgtag cgcacgatct
      181 tcgtgtgcgc cgtctcggga tcgacctcga cgtcggcgac atgcacgccg aagctcggcg
      241 cagcgccatg cgccgtgagg ctcgagtagc cgacgatcgg gccaccggtc ttgccggcgg
      301 cacgcgccac gtccttcagg ctcatcggct tgtgcttgcc ggcgttgtca ccggcaggct
      361 tcacttcgcc gttctcgaag gtgacggcgt cgggcgagag ctcccacagc ttggcggcgc
      421 ggctgcacgc ttccttgatg atggagcgcg ccgcctcgac cgtcgccatg ccgctcgaga
      481 aggtcgaacg gctgccgcct gtcaggaagt tgtagccgag cgaggaggtg tcagcgatga
      541 tcggcttgat gctgtcgacc gacacgccga gctcttcggc ggccatcatg caaagggagg
      601 cgcgcaggcc gccgatgtcg ggcgtacccg ccatcagcgt catggtgccg tcctcgttga
      661 gttgcagcga cacagtcgtc tcgccgccga tgttgaacca gaagccggca gcgatgccgc
      721 ggccctgatt gggcttcagc ggcgtcttcc aatgctcgct cgccttggcc gcctcgagac
      781 actcgacgag accgacgggg ccgaatttcg ggccgtatgc cgccttggtg ccctcgcgcg
      841 cggcattctt gaggcggagc tcgatcgggt ccatgccgat cttctcggca agctcgtcca
      901 cgacgcattc gacagcgtac tccgagatcg gaccgcccgg cgcgcgatag gccgcgacct
      961 tcggacgatt ggagacgaca tcgtagccga cgaccttgac gtgctcgagg tcgtagggcg
     1021 cgaaggcgca catggcgccc ggctgcacgg gcgagccctg gaaggcgccc gcctggtact
     1081 tgaggtcggc cgcggctgcg acgatgcggc cgtcgcgctt ggcgcccatc ttcacccaaa
     1141 cggtcgcgcc agaggttggg ccggaggcgc ggaacacctc ctcgcgcgac atcaccatct
     1201 tgaccgggcg gcgcgccttc ttggaaagcg cgagcgccac tggctcgagg tagacgaccg
     1261 tcttgccgcc gaagccgcca ccgatctcgg acgacgtgac gcgtagcttc gagatgtccc
     1321 agccaagcag ctttgcgcag tgcgcgcgct tgatccaatg gccctgcgtc gtcgtccaga
     1381 gctcggcgag cccgtcttcg ttgacgctcg cgacgcacgc gtgcggctcg atgtagccct
     1441 ggtgcacggg cttcgtcttg aactcgcgct cgacgatcac gtcggcgtcc ttgaacgcct
     1501 cgtccgcgtt gccgagcgtg aactcgacgc gcttcgccac gttcgatgcc gcggccggtg
     1561 gcggcgtcac gccggcggtg atcaggtcct cgtgcaggat cggcgcgccg ggcttcatcg
     1621 cctcgacctc gtcgatcacg tgggggagca cctcgtagtc taccctgatg agcttcaagg
     1681 cggccttggc gacgctgtcc gagatggcgg caaccgccgc gaccggatgg ccttcgaaca
     1741 gcgccttctc gcgcgccatg atgttgcgca cgacgtcgcg gtagttgatc agcatctcgc
     1801 ccgcgggcac gaactcggag ggctgatcgt tgaagtcggc gccggtgacg accgccttca
     1861 cgccgggaag agcggcggcg gccttggtgt cgatcgagcg gatgcgggcg tgcgggtgtg
     1921 ggctcctcaa caccttaccg acgagttgcc ccgccatctt catgtcggcg ccgaaacgcg
     1981 cgcgcccggt gaccttgtcg acgccatccg ggcggacggg gcgtgtcccg atgatcttgt
     2041 agttgggctg cccgccctta ttcactttct cttgcatcgt aaagctcctt ggcttcgagg
     2101 catcaaacgt cgaattggtc cgccggaacc gtcggcatcg tctgcgccga tctcaacggc
     2161 cgcgcagcgg cgctcggccg ccgcgctacg ctcaggtgct ccggcgcatc tcggccgccg
     2221 catcctgaac cgcccggatg atcttgtcgt agcccgtgca gcgacagaga ttgccggcaa
     2281 gccaatagcg gatctcggtc tcggtcggat tggggttctt ctcgagcagc gccttggccg
     2341 cgacgaggaa ccccggcgtg cagatgccgc actgaagggc tgcatgctcc aggaacttct
     2401 tctgcagcgg gtgcaggttg tcgtccttgg ccatgccctc gatggtctcg atcttccgcc
     2461 cgttcgcctc gacagcgagc acgaggcagg agcacacgag acgcccgtca acagtcacgg
     2521 agcaggcccc gcaatcgccg gacgagcagc cttccttggc acccgtcagg ctcagctcgt
     2581 cgcgcaggca gtcgaggaga gtttgcgtcg gctcgcagag aaattccacc ggatcgccgt
     2641 tcacggtggc ggagacgtaa tacttgctca tgatcagcgt ttccttgcgc gctcgagcgc
     2701 gatggcggcg gtgcggcggg cgaggacgcc agccacgtca atacggaact ccttggtgcc
     2761 gcgcttgtcg tcgataggac ggcaggcggc gcgggcagcg gcgtcgagcg cttgcatggc
     2821 ggcatcgtcg acaggcgttc cgatcagcgc cttggcggcg gcatcgacga gcagcggccg
     2881 ctcggcgacc gcgccaaggg cgacgcgcgc cgcagtgcat ttgccggcgc catcgatggt
     2941 aagcgcgacg ccgcaaccga cgacggcgat gtccatctcc gtgcgcggga tgaagc
//
LOCUS       DAORNH010000405         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_24917_length_2990_cov_2.745145, whole genome shotgun sequence.
ACCESSION   DAORNH010000405 DAORNH010000000
VERSION     DAORNH010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24917_length_2990_cov_2.745145"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..178)
                     /locus_tag="PK264_24230"
                     /pseudo
     CDS             complement(1..178)
                     /locus_tag="PK264_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-protoporphyrin IX monomethyl ester
                     cyclase"
     gene            150..344
                     /locus_tag="PK264_24235"
                     /pseudo
     CDS             150..344
                     /locus_tag="PK264_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            352..1140
                     /locus_tag="PK264_24240"
     CDS             352..1140
                     /locus_tag="PK264_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRD79004.1"
                     /translation="MTGSIRLFSGRHALVTGGGSGIGAAVARSLLEAGARVTIAGRRR
                     AVLEQAAGDIGLAALDCFGMDVTDARSVTSGVAAAVARHGRVDILINNAGRATSAPFG
                     KATVGMLDDMLTANVRGTWLVTEAVLPGMIGQKWGRIVNVASTAGLVGYAYATAYVAA
                     KHAVVGLTRALALEVARQGVTVNAVCPGFTETPLLDGAVANITAKTGRSDAEARSDLA
                     RGNPLGRLVQPQEVADAVLWLASPGAAAINGQAVAVCGGEVMTG"
     gene            1145..1987
                     /locus_tag="PK264_24245"
     CDS             1145..1987
                     /locus_tag="PK264_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HRD79005.1"
                     /translation="MSKPMQSALRPMQGYAARHFRWEPSADGRVATIRLDRPEKKNPL
                     TFESYGELRDLFRDLVYASDVRAIVLTGSGGNFCSGGDVFEIIEPLTRMTMPDLLAFT
                     RMTGDVVKAMRRCPQPIIAAVDGVCAGAGAILAMASDIRFATPEAKTAFLFTRVGLAG
                     ADMGACGMLPRIIGQGRAADLLYSGRAFSAAEGHAWGFYNRLVAAAKLDEDANAYARA
                     LADGPWFAHGITKTMMNQEWAMGLDEMIESEAQAQAICMATGDFKRAFEAFAAKSKPK
                     FEGN"
     gene            1989..2393
                     /locus_tag="PK264_24250"
     CDS             1989..2393
                     /locus_tag="PK264_24250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRD79006.1"
                     /translation="MHKTLQPDGWTKPVGYANGVAARGELIFVGGQIGWNERCEFETD
                     DLVGQVAQTLRNIVAVLAEAGARPEHITSMTWYLVDKADYLRNLKDIGRAYREIIGRH
                     FPAMAAVQVVALVEDRAKVEIQAFAVLPDGVS"
     gene            2713..>2990
                     /locus_tag="PK264_24255"
     CDS             2713..>2990
                     /locus_tag="PK264_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HRD79007.1"
                     /translation="MALILPGEPAFDQPREGTGGPARAGGLVKPGDRGFTELSNKLAD
                     KGFLVTTTDDLINWARTGSLMWMTFGLACCAVEMMQASMPRYDVERFG"
BASE COUNT          525 a          998 c          989 g          478 t
ORIGIN      
        1 gccagcgcgt ccagaatccg cgctgaccgc aataattgca gcgatgcgga cagccccgag
       61 agaattgcat gacgacggcg cgcataccgc cgtagtatcc atagtttttg tgatcgatca
      121 gttcccagcc gacgcggtac gcatccaaat cctcgatcat cgccgcgggc cgagccgatc
      181 tctgtgccat cgcgcgtccg catctcgccg atcccgcctg gacgctgcac gaggcggcaa
      241 agatcggcgt ccaggacatc gcatggccga agcagtatct ctcgggcaaa tcgcaatacg
      301 aaacgagtct cgcacgcacc gcgtcaacgc cgacaagaag ctgagacgac catgacgggc
      361 agcatcagac ttttctcggg ccgtcatgcc ctggtaacgg gcggcggaag cggtatcggc
      421 gctgcggtcg cgaggtcgct gttggaggcc ggagcgcgcg tgacaattgc cggccgccga
      481 cgcgccgtcc tggagcaggc cgcgggcgac atcggacttg cagcactcga ttgcttcggc
      541 atggacgtca ccgacgcccg cagcgttacg agcggggttg cggcagcggt cgcacggcat
      601 gggcgggtcg acatcctcat caacaatgcc ggccgcgcga cgagcgcgcc attcggcaag
      661 gcaacggtcg gcatgctcga cgacatgctg acagccaacg tcagaggaac ctggctcgtg
      721 acggaagcgg tcctgcccgg catgatcggg cagaagtggg gccgcatcgt caacgtcgcc
      781 tccaccgcgg gcctcgtcgg ctacgcttac gcgaccgcct atgtagcggc gaaacacgcc
      841 gtcgtcgggc tgacgcgcgc cctggcgctc gaggtcgcgc gacagggtgt gacggtcaac
      901 gcggtctgtc ccgggttcac cgagacgccg ctgctcgacg gcgccgtcgc caacatcacc
      961 gcaaagacag gtcgcagcga cgccgaagcc cgctcagatc tcgcgcgcgg caatcccttg
     1021 gggcggctcg tgcagccgca ggaggttgcc gatgccgtgc tctggctcgc ctcgccgggg
     1081 gcggccgcca tcaacggtca agccgtcgcg gtctgcggcg gcgaagtcat gacgggatag
     1141 cgccatgtcg aaacccatgc agtcagcact gcgtccgatg cagggctatg cggcccgcca
     1201 tttccgctgg gagcccagtg cggacggccg ggtcgcgacc atccgcctcg accggcccga
     1261 gaagaagaac ccgctgacgt tcgagagcta cggggagctg cgagatctct tccgcgatct
     1321 cgtctatgcg tcggacgtac gcgcgatcgt gcttacgggc tcgggcggca atttctgctc
     1381 gggcggcgac gtgttcgaga tcatcgagcc actcacccga atgacgatgc cggatctgct
     1441 ggcgttcacg cgcatgaccg gcgacgtcgt caaagccatg cgcagatgtc cgcagcccat
     1501 catcgccgcc gtcgacggcg tgtgcgcagg cgccggcgcc attctggcca tggcgtccga
     1561 catccgcttc gcgacgcctg aagcgaagac tgcatttctc tttacgcgcg tcggcctcgc
     1621 gggcgccgac atgggggctt gcgggatgct gccgcgcatc atcggccagg gccgggcggc
     1681 ggacctgctg tattcgggcc gcgccttttc ggccgccgag ggtcacgcgt ggggctttta
     1741 caatcgcctg gtcgcggcgg ccaagctcga cgaggacgcc aacgcctatg cccgcgcgct
     1801 cgccgacgga ccctggttcg cccacggcat caccaagacg atgatgaacc aggagtgggc
     1861 catgggcctc gacgagatga tcgaatcgga ggcccaggcg caggccatct gcatggccac
     1921 cggtgacttc aagcgcgcct tcgaggcatt cgccgccaag tccaagccga agttcgaggg
     1981 caactgacat gcacaagacg cttcagcctg acgggtggac gaagccggtt ggctatgcca
     2041 acggcgttgc cgcgcgcggc gagctgatct tcgtcggcgg ccagatcggc tggaacgagc
     2101 gttgtgagtt cgagacggac gacctcgtcg gccaggtggc gcagacgctg cgcaacatcg
     2161 tcgccgtcct cgccgaggcc ggcgcgagac ccgagcacat cacgtccatg acctggtatc
     2221 tcgtcgacaa ggctgattat ctccggaacc taaaggacat cggccgggcc tatcgtgaga
     2281 tcatcgggcg gcacttcccg gcgatggctg cggtgcaggt cgtggccctt gtcgaggatc
     2341 gcgccaaggt ggaaatccag gccttcgccg tgctgcccga tggggtctcg tgacaccatc
     2401 gtcgtacacc ggggccacgc tctgacatca ccgacgagcc tcaggcgctg cccttgccga
     2461 ggtgacaacg tcgtttcatc ctcgcgaagt cgcggaacca gatcacggct cgagcgaacg
     2521 gaagcagccg cttcgagcgt cacatgagtg cccgacccgg ccggcgttac ggtgggaggt
     2581 ggggcttgct gtcacggcac acgcgcatcc ggagcccgcg gacagcggcg ccacacaccc
     2641 gcagacgatc attgccagcc gcagcggcct gggttacctg ctgagtgcag atatggtcag
     2701 ggaccgacga cgatggcatt gatactcccc ggagagccgg cgttcgacca gccccgcgaa
     2761 ggcacgggcg gtccagcccg cgcgggcggg ctcgtcaagc ccggcgaccg cggcttcacg
     2821 gagctttcga acaaactcgc cgacaagggc ttcctcgtca ccacgacgga cgacctcatc
     2881 aactgggccc gcaccggctc gctgatgtgg atgacctttg gcctcgcctg ctgcgccgtc
     2941 gagatgatgc aggcctcgat gccgcgctat gacgtcgagc gtttcggctt
//
LOCUS       DAORNH010000406         2975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_25092_length_2975_cov_2.552397, whole genome shotgun sequence.
ACCESSION   DAORNH010000406 DAORNH010000000
VERSION     DAORNH010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25092_length_2975_cov_2.552397"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK264_24260"
     CDS             complement(<1..211)
                     /locus_tag="PK264_24260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRD79008.1"
                     /translation="MNRDLDLALLRAFAAVVETGSVTSAAHTLNRTQAAVSQQLKRLE
                     DTLGQPLFERARKRMILTPAGEQLIG"
     gene            342..587
                     /locus_tag="PK264_24265"
     CDS             342..587
                     /locus_tag="PK264_24265"
                     /inference="COORDINATES: protein motif:HMM:NF018294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HRD79009.1"
                     /translation="MSKIIANALPGALELGRPVGNRLVAILGHVGLAFRVAAERRQLA
                     ALDDASLKDIGLSRSVAYREASRSILDLPADRLPPAE"
     gene            complement(524..901)
                     /locus_tag="PK264_24270"
     CDS             complement(524..901)
                     /locus_tag="PK264_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79010.1"
                     /translation="MLLSTAAIAGIAAAAGLAAGRRPAGTIGWPLALVWALVQIPVYT
                     TVESGWINQTALRFPLALTYSLRENNQLVSHLQAGVNLAALAYAVMLSRRRQEIASPY
                     AARHSAGGSRSAGRSRIEREASR"
     gene            1584..2291
                     /gene="tolQ"
                     /locus_tag="PK264_24275"
     CDS             1584..2291
                     /gene="tolQ"
                     /locus_tag="PK264_24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HRD79011.1"
                     /translation="MPEAIKSTLAAPGAHSSFLEMFLQAHLVVKIVMVGLLLASVWSW
                     AIIIEKMFAFRRARAEADKFEQMFWSGQSLEELYTALARGRTISMASLFVAAMREWKR
                     SVEGNIRALGGIQLRVEKVMDVTISREMEQLDRRLLFLATVGSTAPFVGLFGTVWGIM
                     TSFQAIASSKNTSLAVVAPGIAEALFATALGLLAAIPAVIAYNKYSADSARLAQRLEA
                     FADEFSAIVSRQIDVRT"
     gene            2322..2789
                     /locus_tag="PK264_24280"
     CDS             2322..2789
                     /locus_tag="PK264_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HRD79012.1"
                     /translation="MGASLNQGAGGKGGRRARARRHLPMAEINVTPFVDVMLVLLIIF
                     MVAAPLLTSGVPVELPSAAGKPLDAPKEPPVVVTIDEKGQIFIGQDDKAPLKVEELGT
                     RLTGIAKNRRGSDEPIFVHGHKSTNYGTFAVVMAAIKEAGFKKLSLVTEPPNR"
BASE COUNT          498 a          927 c         1024 g          526 t
ORIGIN      
        1 gcccgatcag ctgctcgccg gcgggcgtca ggatcatgcg cttgcgcgcg cgctcgaaca
       61 gcggctggcc gagcgtgtcc tcgagccgct tcagctgctg gctgacggcc gcctgcgtgc
      121 gattgagggt gtgggccgcg ctcgtcacgc tgccggtctc gacgacggcg gcaaaggcgc
      181 gcaggagggc cagatcgagg tcgcggttca tggttccttc atgtatcatg aagcgttatg
      241 gatatcatat tacgaattag atttccttat tttccgaatc tgcgatggtg ggacgaagca
      301 gcggcgcgcc cctcggcggt cgcgggattt ggaggcggac catgtcgaag atcattgcca
      361 atgcattgcc gggtgcgctc gagctgggcc gccctgtcgg caatcggctc gtggcgattc
      421 tcggccacgt cgggctcgct tttcgcgtgg ccgcggagcg gcgccagctc gccgcgctcg
      481 atgatgcaag cctgaaggac atcggcttga gccgctcggt cgcctatcgc gaggcctcgc
      541 gctcgatcct cgatctgccc gcggaccggc tgccgccggc cgagtgacgg gcggcatatg
      601 gcgatgcaat ctcctgcctg cgccgggaca gcatcacggc gtaggcgagg gccgcgaggt
      661 tcacgccggc ctggagatgg ctcaccagct ggttgttctc tctgagcgaa tatgtcagcg
      721 ccagcgggaa gcgcagggct gtctggttga tccagccgct ctcgaccgtc gtgtagacgg
      781 ggatctggac gagcgcccag acgagcgcca gcggccagcc gatggtgccg gctgggcgcc
      841 gtcccgcagc aagtccagcc gcggcggcga taccagcaat cgcagcggtg ctgagaagca
      901 cctggcggcg ataccaagca gagcaaccga gacccggcct gcttccggca acttgagggc
      961 aagcgcaccc tggcgatcca gatagagcgt gatcagcgaa gcaatgagcg cgagcgccgc
     1021 aaagatcagg cggagggtgg tgctggtcat tggggaaagt ccggctctgg gaaatgtcgt
     1081 tgctggcaga ctatagttca tgtgctgccg gtcaaggcac gatcgcggcc gggaggctac
     1141 gagccatccc tggagcctgg caccgtggtt gtgcctcgga cggcggcacg gggtggaagc
     1201 ctcggcgcat cgctatgcga ccgtcggcca ttgctcgtgc acgtcgagac gcccgaccat
     1261 gacaacggta aaggctggcg gtcacccaca gaggcattgg gtcacggcga tcagggcgga
     1321 agccgggatt atgctcgcca acggcacatg aagtcgcaat cgccgcgggt gcccgggtcg
     1381 cagtttggtc acatgggccg tcgataagcg gagatgctgt ccgaggtgcc ggcaggagtt
     1441 caagtcaatt gcgccgtgct tacggctgat agctgtaagc ctcgaaatcg attggtgcgg
     1501 tccgtgctcg tgcgggccgt gcagacacga gagggaactc cggacgaccc cgccgagggc
     1561 cgccggcgga acaatggacg gtcatgcctg aagccataaa gtcgacgctt gccgcgcccg
     1621 gggcgcacag ctcgttcctc gagatgttcc tgcaggcgca ccttgtcgtc aaaatcgtca
     1681 tggtcggact gctgctcgcc tcggtgtggt cctgggcgat catcatcgag aagatgttcg
     1741 ccttccgccg cgcccgcgcc gaggccgaca agttcgagca gatgttctgg tccggccagt
     1801 cgctcgagga gctgtacact gcgctcgccc ggggccgcac catctcgatg gcctcactgt
     1861 tcgtcgcggc catgcgcgag tggaagcgct cggtcgaggg caacatccgt gcgctcggcg
     1921 gcatccaact gcgcgtcgag aaggtgatgg acgtcaccat cagccgcgag atggagcagc
     1981 tcgatcgtcg cctcctgttc ctcgcgacgg tcggctcgac agcgccgttc gtcggtctgt
     2041 tcggaacggt gtggggcatc atgacgagct tccaggcaat cgcctcctcc aagaacacga
     2101 gcctcgcggt cgtcgccccc ggcattgccg aagcgctgtt cgcgacggca ctcggcctgc
     2161 ttgccgccat tccggcggtg atcgcctaca acaagtactc ggccgattcg gcacgcctcg
     2221 cgcagcgcct cgaagccttc gccgacgagt tctcggcgat cgtctcgcgc cagatcgatg
     2281 tgcgcacctg agttcgaccg gaaccgcgaa aggagacgcc gatgggcgcc tcgttgaatc
     2341 aaggagctgg cggcaagggt ggacggcgag cccgcgcccg ccgtcacctg ccgatggccg
     2401 agatcaacgt cacgccgttc gtcgacgtga tgctcgtcct tctgatcatt ttcatggttg
     2461 cggcgccgtt gttgacgtcg ggcgtgccgg tcgagctgcc gagcgcggcg ggcaagccgc
     2521 tcgatgcgcc aaaggagccg ccggtcgtcg tgaccatcga cgagaagggg cagatcttca
     2581 tcggccagga cgacaaggcg cccctgaagg tcgaggagct cggcacgcgg ctcaccggca
     2641 ttgccaagaa ccggcgcggc agcgacgagc cgatcttcgt gcacggtcac aagtcgacga
     2701 attacggtac gttcgcggtc gtcatggcgg cgatcaagga ggcgggcttc aagaagttgt
     2761 cgctcgtcac cgagccgccg aaccgctgac tctggtcggg tcacagagga cggagccttg
     2821 agtctcggat tggtcatctc ggccctggtg cacggcgcgc tgctgttctt cgtgctggtc
     2881 gggatgcttg cgccgcgcga cgtgaaggcg ccggagcccg agcccgtcgc tgtgtcactc
     2941 gtcacatccg ccgagttcgc gcgcatgatg aaggg
//
LOCUS       DAORNH010000407         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_25258_length_2960_cov_2.950430, whole genome shotgun sequence.
ACCESSION   DAORNH010000407 DAORNH010000000
VERSION     DAORNH010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25258_length_2960_cov_2.950430"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..770)
                     /gene="def"
                     /locus_tag="PK264_24285"
     CDS             complement(258..770)
                     /gene="def"
                     /locus_tag="PK264_24285"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRD79013.1"
                     /translation="MSVLPIITLPDPILRKPSSPVEQVDDAVRKLAADMLETMYDAPG
                     VGLAAVQVGVLKRLLVLDIAGDGEPPQPIVMINPQILRLGDGRRSHEEGCLSIPDVRI
                     EIERPDAVTVRYVDRDGKTIERECEGLLATAVQHEMDHLDGKLIVDFLSRLKRDMIVR
                     KFKKQARGES"
     gene            complement(851..1327)
                     /gene="rimI"
                     /locus_tag="PK264_24290"
     CDS             complement(851..1327)
                     /gene="rimI"
                     /locus_tag="PK264_24290"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRD79014.1"
                     /translation="MNNFNKQISLLWADGSHAADIAQIQGRLFPDQWSQDAIRRLLAN
                     DGATSLVARSGSPLATIGYILAQIAADEAEILSIGVTPDVQRRGVGRQLLRALLRAAW
                     KAEVKSVHLEVAADNRVAEALYKSEGFLEVGRRPEYYARANGPAVDAVRLALKFGE"
     gene            complement(1324..2007)
                     /gene="tsaB"
                     /locus_tag="PK264_24295"
     CDS             complement(1324..2007)
                     /gene="tsaB"
                     /locus_tag="PK264_24295"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRD79015.1"
                     /translation="MITLAFETCYGACSAAIGEADNVSARVIASRLERMATGHAERLP
                     LLIEAVASEAGITLRQLQRIAVTIGPGSFTGIRVGVAMARGLALATGAEVVATTSLAL
                     ISTGAGLAGADHAVAMPGPKGLVAVELFGSTPTPPRLLEPVELAAALPAACRYICGAG
                     SRTAAVELAKIGRCIEARDVVDEPSASTLLRLAGTLSPVSDALLPLYLKEADAKPQID
                     RALPRVPST"
     gene            complement(2041..2634)
                     /locus_tag="PK264_24300"
     CDS             complement(2041..2634)
                     /locus_tag="PK264_24300"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HRD79016.1"
                     /translation="MAEPVTNAVLRQLFLDARTHNKWQPREVPDALLREIVDHLRWGP
                     TSANCQPARIVFVKSAAAKARLKPFLSANNTDKTMAAPVTAIVGHDLRFYEHLPRMFP
                     HNLTARSWFEGKADHIAATAMRNSSLQGAYMILAARAHGLDCGPMSGFDNAGVDREFF
                     PEGTIKSNFICNLGYGDPAGLHARGPRFSFDEMAQIA"
     assembly_gap    2651..2660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          535 a          936 c          959 g          520 t
ORIGIN      
        1 agctcagctg gtatccgtcc ttgagaagcg gatagagcgc cggaatcatc gactggatga
       61 gatcgttcag cagatgggcc gcgctgagcg cgacgaggat cggcatcatc ggtcgagccg
      121 tcgatggggc gactgcgatt gtcatgcgct cgatccttgg tctgtacgac atgccccaac
      181 gtcgacgacg tgtcgcttga cggtcgccgg gaccttgtag tgccccgcct ctggcggcat
      241 gcgagaccgg tccccgttca agactcgccc cttgcctgct tcttgaattt gcgcacgatc
      301 atgtcgcgct tcagccgtga gagaaaatcg acgatcagct tgccgtcgag gtggtccatc
      361 tcgtgctgca cggcggtggc aagaaggccc tcgcattcgc gctcgatcgt cttgccgtca
      421 cgatcgacgt agcggaccgt gacagcatcg ggacgctcga tctcgatgcg cacgtccggg
      481 atggagaggc agccttcctc gtgactgcgg cgtccgtcgc ctagtcgcag gatctgcggg
      541 ttgatcatga cgatcggctg cggcggctcg ccgtcgccag caatatcgag cacgaggagg
      601 cgtttcaaca cgccgacctg cacggccgcg agcccgacgc ccggcgcgtc atacatggtc
      661 tcgagcatgt cggcagcaag cttgcgcacg gcatcgtcca cctgctcgac cgggctcgac
      721 ggcttgcgca ggatgggatc aggcagggtg atgatcggca ggacggacat gaggtcgaga
      781 tattgtggga tttcccgaac gtcaatctgc acaacgggca gagcaggggg cgatcgagcg
      841 cagcgtccgg ttactcaccg aacttgagcg cgaggcgcac ggcgtcgact gctggaccgt
      901 tcgcccttgc gtaatactcc ggccgtcgtc cgacctcgag gaagccctcg gatttgtaaa
      961 gggcctcggc aacccgattg tcggccgcga cctcgaggtg aactgacttc acctcggcct
     1021 tccaggcggc tcgcaacaga gcacgaagca gctggcggcc gacgcctcta cgctgcacgt
     1081 cgggtgtcac gccgatggag agaatttccg cctcgtccgc agcgatctgc gcaaggatgt
     1141 agccgatggt cgcgagcggc gatcccgagc gtgctaccag cgacgtcgcg ccgtcattgg
     1201 cgaggagccg ccgtattgca tcctgcgacc attgatcggg aaacagccga ccctggatct
     1261 gcgcgatgtc cgccgcatgg cttccgtcgg cccacaagag gctgatctgt ttgttaaaat
     1321 tgttcatgtc gacggaactc tcggcagagc gcgatcaatc tgaggcttcg cgtcagcttc
     1381 tttcaggtag agggggagca gagcatcaga aaccggcgaa agcgtgcccg ccagtctcag
     1441 cagcgtcgaa gcgctcggtt catcaaccac atcccgcgcc tcgatgcatc tgccaatttt
     1501 cgcgagctcg acggcggccg tgcggctgcc agcaccacag atgtaccggc atgccgcggg
     1561 cagagcggcg gcgagctcga caggctcgag caagcggggg ggcgtcggtg tgcttccgaa
     1621 aagctcgact gccaccagcc ccttggggcc aggcatcgcg accgcatgat cggcccctgc
     1681 caacccggcg cccgtcgaga tcagcgccag ggatgtggtg gcgaccacct cggcgccggt
     1741 cgccagcgcc agcccgcggg ccatggcgac gccgacccgg atccctgtga acgagcccgg
     1801 accgatggtg accgcgatcc gctgcagttg gcggagcgta atgcccgcct ccgatgcaac
     1861 ggcctcgatg agcagcggca gccgctcggc gtgccccgtc gccatccgct cgaggcggct
     1921 tgcgatcaca cgagccgaga cgttgtcggc ctccccgatc gcggccgagc aggcgccgta
     1981 gcaggtctcg aacgccaggg tgatcatggt gccgcgcggc caaactcgaa atcgcgaaaa
     2041 tcaggcaatc tgagccattt cgtcgaagct gaagcgcggc cccctcgcat gcaaaccggc
     2101 agggtcacca tagccgaggt tgcagatgaa gttcgacttg atggtcccct ccgggaagaa
     2161 ctcacggtcg acgccggcat tgtcgaagcc cgacatcggc ccgcagtcga ggccgtgggc
     2221 gcgcgccgcc aggatcatat aggcgccctg caatgagctg ttgcgcatcg cggtcgccgc
     2281 gatgtggtcg gccttgccct cgaaccagct gcgcgcggtg aggttgtgcg ggaacatccg
     2341 cggcagatgc tcgtagaacc tgaggtcatg accgacgatc gccgtcacgg gagcggccat
     2401 ggtcttgtcg gtgttgttgg cggagagaaa cggcttcaag cgggccttgg cggccgcgga
     2461 cttgacgaac acgatcctcg ccggctggca attggcgctc gtcggccccc aacgcaaatg
     2521 gtcgacgatt tctcgcagca gcgcgtccgg cacctcgcgg ggctgccact tgttgtgggt
     2581 ccgagcgtcg agaaacagct gccgtagaac cgcgttcgtc acaggctcgg ccatctcgtc
     2641 ctccgttgct nnnnnnnnnn gtggcgtcgc gccaaactgg tcgggccgca cttaagccca
     2701 gttcgtcgac gaaagcgagg ccgaggctcg ccccccgacg ctgctcggcc accagagaag
     2761 atggcgcatg tcggacacag gcctcaaagt tattcggctc ccgtagttca caaggcacga
     2821 taagccgcta taactaaggg agattgtggc gttgcgaatt gatttgtgct gcctacggca
     2881 ctttcgttcg aggcgccggc gacgccgagg tgatcgaatc cagacgttgg atctcattga
     2941 acgggcgctg acccaatacg
//
LOCUS       DAORNH010000408         2924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_25668_length_2924_cov_2.620077, whole genome shotgun sequence.
ACCESSION   DAORNH010000408 DAORNH010000000
VERSION     DAORNH010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25668_length_2924_cov_2.620077"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1320)
                     /locus_tag="PK264_24305"
     CDS             complement(199..1320)
                     /locus_tag="PK264_24305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRD79017.1"
                     /translation="MNQFDTQPGLVGWLMVMLRAAWGWVRVLLHLVPIVWSAIGRIGR
                     RLVVAAAIIAALAALLSDWRVPLRHWMDSGNRPLLAAKSWYFYLDSFEIDRALQNKSD
                     MFVIDHAHNHDNRPLTKEDLARLKARPGEKPRLLISYFSIGEAESYRFYWRKDWDEDP
                     PGWYVAENCAWPRNYMVRFWHDGWKDIIYRGKRSYLARIIEAGFDGVYLDRVDVFDNL
                     TEERPTAKEDMIDFVIELARTARKLKPGFIVIAQNGEDLLRSERYRAAIDGLGKEDLL
                     YGVHGTEKRNPKREIDDHWRNIRLLLRDWKPVFAVEYVAKPELISSARKELQSLGLVP
                     TFAHRSLDGYDPGHPRPPPKIEYGTPEWVAANCKDKKHW"
     gene            complement(1477..2924)
                     /locus_tag="PK264_24310"
                     /pseudo
     CDS             complement(1477..2924)
                     /locus_tag="PK264_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822085.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
BASE COUNT          491 a          927 c          982 g          524 t
ORIGIN      
        1 cggcttcggc gccggcgcct gtgcggaggc aatcgcaaat gcggatacgg cgagggcagc
       61 ggcaacgcca gtcaccgtgg cgagccttgt aacgcgaggc aacatgagaa ctcctgttcg
      121 acgaccagtg atttcttacc atggacgccg tgagccgatc aggaccccgc gtcaatctga
      181 ctacggccat tgcggccgct accagtgctt cttgtccttg caattcgcgg cgacccactc
      241 gggcgtgccg tactcgatct tcggcggcgg gcgagggtgt cctggatcat atccatcgag
      301 cgagcgatgc gcgaacgtcg ggacgaggcc gaggctctgg agctctttcc gagccgagct
      361 gatcaactcc ggcttggcga cgtactcgac tgcgaagacc ggcttccagt cgcgcagcag
      421 caggcggatg ttgcgccagt ggtcgtcgat ctcgcgcttc ggattgcgct tctccgtccc
      481 gtgcacgccg tagagaaggt cctccttgcc gagtccgtcg atcgccgcgc gatagcgctc
      541 cgagcgcagg aggtcctcgc cgttctgcgc gatgactatg aagccgggct tgagtttgcg
      601 tgccgtccgg gcaagctcga tgacaaagtc gatcatgtcc tccttggccg tcggacgctc
      661 ctcggtcagg ttgtcgaaga cgtcgacgcg gtcgaggtag acgccatcga atccggcctc
      721 gatgatccgc gcgagatagg agcgcttccc gcgataaatg atgtccttcc agccgtcatg
      781 ccagaagcgg accatgtagt tgcgcggcca ggcgcaattt tcggccacat accagcccgg
      841 cgggtcctcg tcccagtcct tgcgccagta gaaacgatag ctctccgcct cgccgatcga
      901 gaagtaggag atgagcaggc ggggcttttc gccgggtcgc gccttgaggc gcgcgaggtc
      961 ctctttcgtg aggggacggt tgtcgtgatt gtgggcatgg tcgatgacga acatgtccga
     1021 cttgttctgc aaggcccggt cgatctcgaa gctgtcgaga tagaagtacc agctcttggc
     1081 cgcgaggagc ggtcggttgc cactgtccat ccagtgtctg agcggcacgc gccagtccga
     1141 gagcaaggcg gcaagcgctg cgataattgc agcggcaacg acgaggcgcc ggccgatgcg
     1201 gccgatggcg gaccagacta tgggaacgag gtgcaaaagc acacgcaccc agccccaggc
     1261 ggcccgcagc atcaccatca accatcccac gagcccgggc tgggtatcga actggttcat
     1321 cgtcgttttc ccgaagcaag cgcggccgag acagtgccgc tccggtgctg cgttggcaac
     1381 cccggattgg cgatgtggga catgcgcgcg ctccccgtta gtacataggg ttagcggcgg
     1441 acatcctcgg gcatgatccg ggtaaagaca gaacgatcag tgaatttcgg gatccggcgt
     1501 cgccgagccc tgctccagga agtcgaaatc gcagccgtcg tccgcctggc cgatgtgggc
     1561 ctggaacatg gcgccatagc cgcgctcgaa gcggggcttc ggcgccaccc atgcggcctt
     1621 gcggcgtgcc atctcttcgt cgctgatgtc gagattgatc gagcgattgt cgacgtcgac
     1681 ggagatgaag tcgccggtct tgacgagggc gagcggtccg ccgacgaagc tttcgggcga
     1741 gacgtgcagc acgcaggctc catagctcgt gccgctcatg cggccgtcgg acacgcgcaa
     1801 catgtcgcgc acgcctgctt tgacgagctt cctcggcaac ggcagcatgc cccactccgg
     1861 catgccgggt ccgcccttcg ggccagcgtt ctgcaagacc agcacgtgat cgggggtcac
     1921 gtcgaggtcg tcgcgctcga tgagggaggc catgtgattg tagtcggaga acacgagcgc
     1981 gggtccgcga tgtttcctca ggcgcggctc gcaggccgcc ggcttgatga cggcgccgcg
     2041 cggggcgaga ttgcctttca gcacggccgt ggccccgccc gcatagagcg gcgcggaggc
     2101 cggcatgatg acgtcgcggt tgtagacctc ggcgtcggcg atgttctcgc agatagtccg
     2161 gccgttcacc gtcatcgccg agcagtggat gcggtcgccg agctcgcgca tcaacgcgcg
     2221 gatgccgccc gcgtagtaga aatcctccat caggtatttg cccgacggac ggacgttggc
     2281 gatcacgggg atgtcgcggc cgatctggtc gaagcgctcg agagagaggg ctatgccggc
     2341 gcgccgcgcc atggcgacga cgtgcacgat ggcgttggtc gagccgccca tggccatgtg
     2401 caccatgatc gcgttctcga cggccgccgg cgtgatgatc ttgtcgggcg tgagatcctc
     2461 ccacaccatt tcgacggctc gccggcccga ggccgcgcac atgcgcgctg tcagccgcgg
     2521 gcagcgacga ggcgccaggg agcgacaggc cgagcgcctc ggtgatcgcc atcatggttg
     2581 cggccgtgcc catggtcatg caggtgccga aggagcgagc gatccccgct tccatctcgc
     2641 cccattcctg ctcgctgatc ttgccggcgc gcttctcgtc ccagtatttc cacacgtccg
     2701 agcccgagcc gagcgtctgg cctttccagt tgccgcgcag cgccggtccc gccgggcagt
     2761 agatcgcggg gagccccatg ctgattgcgc ccatgacgag gccgggcgtg gtcttgtcgc
     2821 aaccgccgag cagcacggcg ccgtcgaccg gatgcgagcg caggagctct tccgtctcca
     2881 tggcgaggaa gttgcggtag agcatggtcg tcggcttgac gaag
//
LOCUS       DAORNH010000409         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_25754_length_2918_cov_2.427524, whole genome shotgun sequence.
ACCESSION   DAORNH010000409 DAORNH010000000
VERSION     DAORNH010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25754_length_2918_cov_2.427524"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PK264_24315"
     CDS             complement(<1..866)
                     /locus_tag="PK264_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRD79018.1"
                     /translation="MSRPAPDILCLGEAMVEFSAPPRGGVEQGWIQGFGGDTSNCAVA
                     AARQGGRVGYISAVGDDPFGAMLVEMWTREGIDTTAVLRDPSAPTGVYFITHGASGHA
                     FSYLRAGSAASRLTPAALPRQMIGKARVLHLSGISQAISASGADTAFEAMAVARAAGV
                     LVSYDTNLRLRLWPLPRARAVIMAAVAEADIVLPGLEDARQLTGLDDADAIADRFLAG
                     HARVVALTLGKEGALVATRERRERIGSLPVAAIDASGAGDCFDGAFLVEYLRTGDAYD
                     AGRYACAAAALST"
     gene            complement(863..1255)
                     /locus_tag="PK264_24320"
     CDS             complement(863..1255)
                     /locus_tag="PK264_24320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79019.1"
                     /translation="MQLNNAESATAALAVLDRFIAALNQRDEAAINATLNFPHVRIAS
                     GRVAVWQSRGDYTIEGFLSRVSGDGWARSAWTRRDVIHAGADKVHIDTEFSRFRADGS
                     LIASFTSIYIVTRVDGRWGIQARSSFAP"
     gene            complement(1496..>2918)
                     /gene="mutS"
                     /locus_tag="PK264_24325"
     CDS             complement(1496..>2918)
                     /gene="mutS"
                     /locus_tag="PK264_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRD79020.1"
                     /translation="VKRDGGFVRAGYRPDLDDNRRLRDDSRRVLAALEARYQGETGIK
                     SLKVRHNNQIGTYVEVPAQAGKALLDDEAAKIWRHRQTLPNVLRFTTAELVETEGQIA
                     TAADRAIAIENEVFSMLAALIAKEDARLLAIAASLAELDQYAALAELAIEQGYVRPTV
                     DESSAFEIRGGRHPVVEQALRRARAGPFIENDCLLVRGAENADTPRSTPGFDEDVAAR
                     ILIVTGPNMAGKSTYLRQVALITILAQMGSFVPARAAHIGVVDRLFSRVGASDDIARG
                     RSTFMVEMVETAGILNQATERSLVILDEIGRGTATFDGLSIAWAAVEHLHATNRCRAL
                     FATHYHELTALAGRLPDVANVTMDVKEWQDEIVFLHKVRPGAADRSYGIQVAKLAGLP
                     RPVVERASEVLATLEKADGPRGKPGMGALDDLPLFAAARPKSVLPTAPSPVETALADV
                     QPDELSPKQALELVYRLKALAKG"
BASE COUNT          453 a         1013 c         1003 g          449 t
ORIGIN      
        1 gtcgacagcg cggcagcagc gcaggcatag cggcccgcgt cgtaggcatc gcccgtccgc
       61 aggtactcga ccaggaacgc gccgtcgaag caatcgcccg ccccgctcgc atcgatcgcg
      121 gcaaccggca gcgagccgat ccgttcccgc cgctcgcgtg tcgcaacgag cgcaccctcc
      181 ttgccgaggg tcaaggccac gacacgcgcg tgcccggcga gaaatctgtc tgcgattgcg
      241 tccgcgtcat cgagcccggt cagttggcgc gcatcttcga ggcccggcag cacgatatcg
      301 gcctccgcga ccgctgccat gatcacggcg cgcgcccgag gaagcggcca gagccggagc
      361 cgcaggttcg tatcgtagga gacgaggacg cccgccgccc tcgcaaccgc catcgcctcg
      421 aaggccgtgt cggccccgct cgccgaaatc gcctggctga tgccggagag atggagcaca
      481 cgcgcctttc cgatcatctg gcgcggcaac gcggcgggcg taagccggct cgcggccgag
      541 cctgcgcgca aataggagaa ggcgtggccc gacgccccgt gcgtgatgaa gtagacaccc
      601 gtcggcgcac tcgggtcccg cagcacagcc gtcgtgtcga tcccctcgcg cgtccacatc
      661 tcgacgagca ttgccccgaa gggatcatcg ccgactgccg agatgtaccc gacacgcccg
      721 ccctgccgcg ccgccgccac cgcacagttc gacgtgtcgc cgccgaagcc ctggatccag
      781 ccctgctcga ccccgccgcg cggcggcgcg ctgaactcga ccatggcctc gccgaggcag
      841 aggatgtcgg gcgcaggccg gctcacggcg caaagctcga acgcgcctgg atgccccagc
      901 ggccgtcgac gcgcgtcacg atgtaaatag aggtgaagct cgcgatgagg ctgccgtcgg
      961 cgcggaagcg gctgaattcc gtgtcgatgt ggactttgtc ggcgccggcg tggatgacgt
     1021 cgcggcgcgt ccaggcgctc cgcgcccagc cgtcgcccga gacgcgcgac aggaagccct
     1081 cgatcgtgta gtcaccgcgc gactgccaaa cggcaacgcg gccgctcgca atccgcacat
     1141 gcggaaagtt cagcgtcgca ttgatggccg cctcgtcacg ctgattgagc gccgcgatga
     1201 agcgatcgag cacggcgagc gctgcggtcg cgctttcggc gttgttcagc tgcatgcggc
     1261 gtcactccct cgggcgagca ggtctcgttc gcttcatccg acattcgatg ctcgcgcgcg
     1321 gttggtcaat cgatctggtg gatacacgtc aatgggtttg gccgagcgaa cggttcactg
     1381 aactgtcgtg caggaggcgc gccagtgaac agtcgtgccg gaggcgcgca gcttaagatt
     1441 gtgccggagg ccgccgccga gacggctgca ggagccgcgc tcgcgcgacg agttgtcaac
     1501 ccttcgccag cgccttcagc cgatagacga gctcgagcgc ctgcttgggg ctcagctcgt
     1561 ctggctgcac atcggcaagc gcggtctcga cgggcgaagg cgccgtaggc aacaccgatt
     1621 tcggccgcgc ggcagcgaac agcggcagat cgtcgagggc acccatgccc ggcttgccgc
     1681 gcggaccgtc tgccttctcg agtgttgcca gcacctcgct cgcccgctcg acgacgggcc
     1741 gcggcagccc cgcgagcttg gcgacctgga tgccgtagga gcgatcggcg gcaccggggc
     1801 gcaccttgtg aaggaagacg atctcgtcct gccattcctt cacgtccatc gtgacgttgg
     1861 cgacatcggg taggcggccg gcgagagcgg tcagctcgtg gtagtgggtc gcaaacagcg
     1921 cccggcaccg gttcgtggca tgaaggtgct cgaccgccgc ccacgcgatg gaaagcccgt
     1981 cgaaggtcgc tgtgccgcgg ccgatctcgt cgaggatgac gaggcttctc tcggtcgcct
     2041 ggttgaggat gcccgccgtc tcgaccatct cgaccatgaa cgtcgagcgg ccgcgggcga
     2101 tgtcgtcgga ggcgccgacg cgcgagaaga gccggtcgac caccccgatg tgggcggccc
     2161 gcgctggcac gaacgagccc atctgcgcga ggatggtgat cagcgccacc tgccggagat
     2221 aggtcgactt gccggccatg ttgggaccgg tgacgatcag gatgcgcgcg gccacgtcct
     2281 cgtcaaaccc gggggtcgag cgcggcgtgt ccgcgttctc cgcgccgcgc accagcaggc
     2341 agtcgttctc gatgaagggg cccgcgcgcg cgcgcctgag cgcttgctcg acgacgggat
     2401 gccgcccgcc gcggatctcg aaggccgagc tctcgtcgac agtcgggcgc acgtagcctt
     2461 gctcgatcgc gagctcggcc aaggccgcgt actggtcgag ctcggcgagg ctcgccgcga
     2521 tcgcgagcag ccgcgcgtcc tccttggcga tcaaagccgc cagcatcgag aacacctcgt
     2581 tctcgatggc gatggcacgg tcggcggcgg tagcgatctg gccctcggtc tcgacgagct
     2641 cggcggtcgt gaagcggagc acgttcggca gcgtctggcg gtgccgccag atcttcgccg
     2701 cctcgtcatc tagcagcgcc ttccccgcct gtgccggtac ctcgacgtag gtgccgatct
     2761 ggttgttgtg gcggaccttc aggctcttga taccggtctc gccctggtag cgcgcctcga
     2821 gggctgcgag cacgcggcga ctgtcatcgc gcagccggcg attgtcgtcg aggtcggggc
     2881 ggtagccggc gcgcacgaag ccgccgtcgc gcttcacc
//
LOCUS       DAORNH010000410         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_25819_length_2911_cov_2.682773, whole genome shotgun sequence.
ACCESSION   DAORNH010000410 DAORNH010000000
VERSION     DAORNH010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25819_length_2911_cov_2.682773"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="PK264_24330"
     CDS             complement(<1..1437)
                     /locus_tag="PK264_24330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRD79021.1"
                     /translation="MRPAVLMPLFASARSLSGVGPRTEVFLKKALRLPPGVGEPRLVD
                     LAWHLPTGVVDRRASPTVAEAIPGTIATLELRILKHRVPPRGNAKAPYKVSAEDDTGR
                     IDLVFFHAERRFIERQLPVGSTRFVSGRIERYAEQTQMTHPDYIVAPEAKDELPRLEP
                     VYPLTAGLSGKILQKATRQAIEQVPPLQEWQEPRWLAERGWPSFNEAITRLHRPHEPD
                     DVSPASLPWQRLAYDELLAGQIALAIVRQGEKAARGRAVSGDGQVRARIRAALPFALT
                     PSQTTALGEVEADMGSPSRMLRLLQGDVGSGKTVVALMSMAIAVEAGAQAALMAPTEV
                     LARQHAETIQPLAEKAGLRLGLLTGREKGRARREVLERLAAGEIDVLIGTHALFQPDV
                     AFRDLAFAVIDEQHRFGVHQRLALQTKGGDGGANVLVMTATPIPRTLLMTHYGDLDVS
                     RLTEKPAGRKPITTTKVSTERLDDVIDGL"
     gene            1588..1902
                     /locus_tag="PK264_24335"
     CDS             1588..1902
                     /locus_tag="PK264_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HRD79022.1"
                     /translation="MSGPATPQQMPSGEGSNALDARRKRALWRAEHRGTKELDWLVGR
                     FAAAMLAGMSEPELHRFEELLAMPEPDLEAWIMRGDDITGSALAPLIAEMRAFHGLGE
                     SK"
     gene            1899..2819
                     /locus_tag="PK264_24340"
     CDS             1899..2819
                     /locus_tag="PK264_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD79023.1"
                     /translation="MSTRPLPELYPDFATRRITTSGAEIHVRHGGAGLPVILIHGYPQ
                     THACWHRIAADLAREQTVVIPDLRGYGASSVPASDARHEVYSKRAMALDCVEVMRALG
                     HERFAVVGHDRGGRVAYRLALDHPSSVTRLVTLDIVPTLSTWEDTSLKTSVGRFHWPF
                     LAQPAPFPETVIGRDPIHFHEHLIASWTGAKNLSAISPEALDHYRASFTDPDRIHAMC
                     EDYRAGASIDVELDAADRAAGRKITCPLLALWGQGRENAFFSGPLDTWRRWATDVRGR
                     GLVSGHFLPEEAPRETLAEIVPFLRGMTAS"
BASE COUNT          435 a         1010 c         1003 g          463 t
ORIGIN      
        1 gaggccgtcg atcacgtcgt cgagccgctc ggtcgacacc ttggtggtcg tgatgggctt
       61 gcggcccgcg ggcttctccg tcaggcgcga gacgtcgagg tcgccgtaat gcgtcatgag
      121 cagcgtgcgc gggatcggcg tcgccgtcat gacgagcacg ttggcgccgc cgtccccgcc
      181 cttggtctgc agggccaacc tctggtgcac gccgaagcga tgctgctcgt cgatcacggc
      241 gaaggcgagg tcgcggaagg cgacgtccgg ctggaaaagt gcgtgcgtac cgatcagcac
      301 gtcgatctca ccggcggcga ggcgctcgag cacctcgcgg cgcgcgcggc ccttttcgcg
      361 acccgtgagg aggccgaggc gaagccccgc cttctcggcg agcggctgga tggtctcggc
      421 gtgctggcgg gcaagcacct cggtcggcgc catgagcgcg gcctgggcgc ctgcctcgac
      481 ggcgatcgcc atcgacatca gcgcgacgac ggtcttgcct gagccgacgt cgccctggag
      541 gaggcgaagc atgcggctcg gcgagcccat gtcagcctcg acctcgccga gtgccgttgt
      601 ctgcgagggc gtcagcgcga aagggagtgc ggcccggatg cgcgcccgga cctggccatc
      661 ccccgagacc gcgcgaccgc gcgcggcctt ctcgccctgg cgcacgatgg cgagcgcaat
      721 ctggccggcg agcagctcgt cgtaggcgag gcgctgccag ggtaggctcg ccggcgagac
      781 gtcgtcgggc tcgtgcggcc ggtgaagacg cgtgatcgcc tcgttgaacg agggccagcc
      841 ccgctcggcg agccagcgcg gctcctgcca ttcctggagc ggggggacct gctcgatcgc
      901 ctggcgcgtc gccttctgga ggatcttccc cgagagcccc gccgtcagcg gatagacggg
      961 ctcgagccgc ggcagctcgt ccttcgcttc gggcgccacg atgtaatcgg gatgcgtcat
     1021 ctgcgtctgc tcagcgtaac gctcgatccg cccggagacg aagcgggtcg atccgaccgg
     1081 cagctggcgc tcgatgaagc ggcgctccgc atggaagaag acgagatcga tgcggccggt
     1141 atcgtcctcg gcggagacct tgtagggcgc cttggcgttg ccgcgcggcg gtactcgatg
     1201 cttgaggatg cgcagctcga gcgtggcgat cgtgcccgga atggcctcgg ccacggtcgg
     1261 gctcgcgcgc cggtcgacga cgccggtcgg caggtgccag gcgagatcga cgaggcgcgg
     1321 ctcgccgacg cccggcggca ggcgcaacgc cttcttgagg aacacctcgg tacgcgggcc
     1381 gacacccgag aggctccgcg ccgaggcgaa cagaggcatg aggacggctg gccgcatcgg
     1441 ttaggttcga ccttctcctt catcggcgca cgctaccgcg ctagattgca caatgggagc
     1501 gcacgttgct tggtgctgca atcgatcgtc aaggccatgt cgtcaaggcc atgggcgctc
     1561 gtttgtcgtc ttttctgagg agagagcatg tcgggtccag ccacacctca acagatgccg
     1621 tccggggagg gcagcaacgc tctcgatgcc cgccgcaagc gggcgctctg gcgggcagag
     1681 catcgcggca ccaaggagct cgattggctg gtcgggcgct tcgccgccgc aatgctcgcc
     1741 ggcatgagcg agcccgagct ccaccgcttc gaggagctgc tcgccatgcc cgagcccgat
     1801 ctcgaggcct ggatcatgcg tggcgacgac atcacgggaa gcgcactcgc gccgttgatc
     1861 gctgaaatgc gcgcgttcca cggacttgga gagagcaaat gagcacaagg ccgcttcccg
     1921 agctctatcc ggactttgcg acacgtcgca tcacgacgtc gggcgccgag atccatgtcc
     1981 ggcatggcgg tgccggcttg ccagtgatcc tgatccatgg ctatccgcaa acacatgcct
     2041 gctggcatcg cattgcggcc gatctcgcgc gcgagcagac ggtcgtcatc ccggatctgc
     2101 gcggctatgg agcaagttcg gtgccggcga gcgatgcccg ccacgaggtc tactcgaagc
     2161 gtgccatggc cctcgactgc gtcgaggtga tgcgcgccct cggccacgag cgcttcgccg
     2221 tcgtcggcca cgatcggggc gggcgcgtcg cctaccgcct cgccctcgac catccctcgt
     2281 ccgtcacgcg gctcgtgacg ctcgacatcg tacccaccct ctcgacctgg gaggatacct
     2341 cgctgaagac gagcgtcggc cgcttccact ggccattcct cgcgcagccg gcgccgttcc
     2401 ctgagaccgt gatcggccgc gatcccatcc acttccacga gcacctcatc gcctcctgga
     2461 ctggcgcaaa gaacctgagc gccatctcgc ccgaagcgct cgaccattat cgcgcctcct
     2521 tcaccgaccc cgaccgcatc cacgccatgt gcgaggacta tcgcgccggc gcctcgatcg
     2581 acgttgagct cgatgcagcg gaccgcgccg cgggacgcaa gatcacatgt cccctgttgg
     2641 cgctctgggg gcagggccgc gaaaacgcct tcttctccgg cccgctcgac acctggcggc
     2701 gttgggcgac ggacgtgcgc gggcgcggtc tcgtctcggg gcattttcta cccgaggagg
     2761 cgccccgcga gacgcttgcc gagatcgtgc ctttcctcag gggcatgacc gcgtcatgag
     2821 ccggaaggtc acaacccggc cgcagccgcg cctcccggca aagctgccgc ggatcggcct
     2881 cgcgctcggc ggcggcggcg cgcgcgggct c
//
LOCUS       DAORNH010000411         2909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_25844_length_2909_cov_2.775753, whole genome shotgun sequence.
ACCESSION   DAORNH010000411 DAORNH010000000
VERSION     DAORNH010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25844_length_2909_cov_2.775753"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1774)
                     /locus_tag="PK264_24345"
     CDS             complement(<1..1774)
                     /locus_tag="PK264_24345"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HRD79024.1"
                     /translation="MAAVETRNDGNVAILTIDNPPVNALGHAVRVGLSEGLAKALADP
                     AVKAIVVTGAGRAFSAGADITEFRSGMKEPGLGTVIDAIEASPKTVVAAINGLALGGG
                     LEVTLGCHYRVAAKSVAQLGLPEVKIGILPGAGGTQRLPRAIGVAPALDMIVSGAPIG
                     AEAAAKAGLVDKIVDGDVVAAAIAYARELVAAGKGPRPTGARAIDPATNPPGLFDKKR
                     GEIARHPSGPMAPRNCIACVEAATKLPIKEGLVRERELFKELAGGPNARALQYAFFAE
                     RQAANIPDIGADVKPRPVKSVGILGAGTMGVGIGLAVLSAGLPLILVETSQAALDKGV
                     GRIRETIEGNVKRGRLTAADAEARLKSLTATLDLAKLAGADLIIEAVFENMGVKKEVF
                     AKLDAVAKPGAILATNTSTLDVDEIARATKRPGDVIGLHFFSPANIMRLLEIVRGKAT
                     SKDVLATAMAFARKIGKVGVVAGVCFGFIGNRMLEAYLEEAQAMMLEGASPRDIDAAI
                     EAWGFAMGPNAMMDLAGLDVGYRIRKEHALSPERAALYAVTDRIVEMGRHGQKTGAGM
                     YLYAAGDRKGQPDPKIAEMFRAEAE"
     gene            complement(1940..2338)
                     /locus_tag="PK264_24350"
     CDS             complement(1940..2338)
                     /locus_tag="PK264_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79025.1"
                     /translation="MLKAVLTSIAGFAFAVAIAAIGPFTSQPAAALNGASPSLVTDMR
                     TDVPRAFETVAHKSCGSMGTAGRCASGVRSCLASGKPKGVCDAWGKGCTECQTTFISC
                     GKRLGHIKRPFGTCEGCKSQYDACVARLPR"
     gene            complement(2473..>2909)
                     /locus_tag="PK264_24355"
     CDS             complement(2473..>2909)
                     /locus_tag="PK264_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRD79026.1"
                     /translation="GSGIVSLIHVRDLVGARAEIVGVVAEAAPCIALSFEAGRPVPTN
                     SARTFADGMACRDPHPLALEHILAGAARIVRVSEDEIAAAARACFEDTHNVAEGAGAA
                     PLAALIKERATMAGKRVGVVLSGGNIDRPVFAEVLAGRTPKV"
BASE COUNT          450 a          983 c         1007 g          469 t
ORIGIN      
        1 cctcggcctc ggcgcggaac atctcggcga tcttcggatc gggttggccc ttccggtcgc
       61 ccgcagcata gagatacatg ccggcgcccg tcttctggcc gtgccgtccc atctcgacga
      121 tgcgatcggt gacggcgtag agcgcggccc gctcgggcga gagcgcgtgc tccttgcgga
      181 tgcgatagcc gacatcgagg ccggcgaggt ccatcatcgc gttcggaccc atggcgaagc
      241 cccacgcctc gatcgccgcg tcgatgtcgc gcgggctcgc cccttcgagc atcatcgctt
      301 gcgcttcctc gaggtaggcc tcgagcatgc gattgccgat gaagccgaag cagacaccgg
      361 cgaccacgcc gaccttgccg atcttccggg cgaaggccat ggcggttgcg agcacgtcct
      421 tcgatgtggc cttgccgcgc acgatctcga ggaggcgcat gatgttggcc ggcgagaaga
      481 aatggaggcc gatcacgtcg cccggccgct tcgtcgcccg cgcgatctcg tcgacatcga
      541 gtgtcgaggt gttggtcgcg aggatcgcgc ccggcttggc gacagcgtcg agctttgcga
      601 acacctcctt cttgacaccc atgttctcga acaccgcctc gatgatgagg tcggcgccgg
      661 cgagcttggc gaggtcgagc gtcgcggtca gtgatttgag gcgcgcctcg gcgtcggcag
      721 ctgtgagccg gccgcgcttc acgttaccct cgatcgtctc gcggatgcgg ccgacgccct
      781 tgtcgagcgc cgcttgcgac gtctcgacga ggatcagcgg cagcccggcg ctgagcaccg
      841 cgaggccgat gccgacgccc atggtgccgg cgccgaggat gccgacagac ttcacggggc
      901 gcggcttcac gtccgcgccg atgtcgggga tgttggcggc ctggcgctct gcgaagaagg
      961 catactggag ggcgcgcgca ttgggcccgc cggcaagctc cttgaagagt tcgcgctcac
     1021 gcacgagacc ttccttgatc ggcagcttcg tcgcagcttc gacgcaagcg atgcagttcc
     1081 tcggcgccat ggggcctgac gggtgacggg caatctcgcc gcgctttttg tcgaagaggc
     1141 cgggcggatt ggtcgccgga tcgatcgcac gcgcgccggt cgggcgcggg ccttttcccg
     1201 cggcaacaag ttcacgggcg taggcgatgg ccgcggcgac gacatcgccg tcgacgatct
     1261 tgtcgacgag gccggccttg gcggcggcct cggcgccgat cggggcgccg gagacgatca
     1321 tgtcgagcgc gggggcgacg ccgatcgcgc gcggcaggcg ctgtgtgccg ccggcgccgg
     1381 gtaggatgcc gatcttcacc tcgggcaagc cgagctgggc gaccgacttc gccgcgaccc
     1441 gatagtgaca gccgagcgtg acctcgaggc cgccaccgag tgccagacca ttgatcgccg
     1501 cgaccaccgt cttcggcgag gcctcgatcg catcgatcac cgtcccgagg cccggctcct
     1561 tcatgcccga gcggaactcg gtgatgtcgg cgcccgccga gaaggcgcgg cctgcgcccg
     1621 tcaccacgat cgccttcacc gcgggatcgg cgagcgcctt agcgagccct tccgagagac
     1681 cgacacggac ggcatgcccg agcgcgttta cgggcggatt gtcgatggtg agaatggcga
     1741 cgttgccgtc gttgcgtgtc tcgaccgcgg ccatcggaat tctcctgctg tcgtcggatc
     1801 tccctcgccg cactctgtca cttcgggctg caccggggaa ggcccgaagc ctcgccgcgc
     1861 cgcatgcgag agccgcgcgg cacaattggc cgcggcgccc gtcgcgagcc gcaagatttc
     1921 aggcacaggg cgacaaggct cagcgcggca ggcgtgcgac gcaggcgtcg tattgcgact
     1981 tgcacccctc gcaggtcccg aacgggcgct tgatgtggcc tagtctcttg ccgcaagaaa
     2041 tgaaggtggt ttggcactcg gtgcagccct tgccccacgc gtcgcagacg cccttgggct
     2101 tgccgctcgc gaggcagctg cgcacgccgc tggcgcagcg gccggcggtc cccatgctgc
     2161 cgcagctctt gtgggcgacc gtctcgaaag cgcgcggcac gtcggtgcgc atgtccgtga
     2221 cgaggctcgg cgatgcaccg ttgagcgcag cggccggctg actggtgaac ggcccgatag
     2281 cggcgatcgc gacggcgaac gcgaagccag cgatactggt caggactgcc ttgagcaaga
     2341 tggactctcc attggatcaa ttgaccggaa cacttccgtg atcctgccat ggagagcgta
     2401 gcactgacag agttggtgag tcacgacatc accgcgattt gggaacgcgg agacttggtc
     2461 agcaaagcgc gttcacacct tcggagtgcg cccggcgagc acctcggcga acaccggccg
     2521 gtcgatgttg ccgcccgaaa gaacgacgcc gacgcgcttt ccggccatcg tcgcgcgctc
     2581 cttgatgagg gccgcgagcg gcgcggcacc agcgccctcg gcgacgttgt gcgtgtcctc
     2641 gaagcaggcg cgcgcggcag ccgcgatctc gtcctcgctc acgcggacga tacgcgcggc
     2701 gcccgcaaga atatgctcga gcgcgagagg atgcggatcg cggcacgcca tgccgtccgc
     2761 aaacgtgcgg gccgagttcg tcggcacggg ccggcccgct tcgaaggaga gcgcaataca
     2821 gggcgcggcc tctgcgacca cgccgacgat ctcggccctg gctccgacga gatcgcggac
     2881 gtggatcagg ctgacgatcc ccgagccca
//
LOCUS       DAORNH010000412         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_26291_length_2873_cov_2.640525, whole genome shotgun sequence.
ACCESSION   DAORNH010000412 DAORNH010000000
VERSION     DAORNH010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26291_length_2873_cov_2.640525"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..2514
                     /gene="ligA"
                     /locus_tag="PK264_24360"
     CDS             124..2514
                     /gene="ligA"
                     /locus_tag="PK264_24360"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRD79027.1"
                     /translation="MRKPTKSGRTAIETPAEDLDEGAAARELERLAGLIAHHDRLYHQ
                     QDAPEISDAEYDRLRQRNELIEQRFPALVRADSPTQRVGATPAESFGKVRHRVPMLSL
                     GNAFAEADVVDFLARINRFLGLEASAELEFSAEPKIDGLSISLRYEAGELVEAATRGD
                     GAEGENVTANVRTIRSIPHRLAGKRHPAVIEVRGEIYLSHDAFRALNEAQAAAGEKVF
                     ANPRNAAAGSLRQLDASVTARRPLAFFAYAWGEASALPGDTQEAICRSFEAWGLPVNE
                     RRRVVNGAEALIAFYREIGAVRASLGYDIDGVVYKVNRLDLRERLGFVSRAPRWAIAH
                     KFPAEQATTILEAIDIQVGRTGALTPVAKLRPVTVGGVVVSNASLHNEDEIARKDVRI
                     GDTVIVQRAGDVIPQVLGVVGEKRPPGTMPYEFPRTCPACGSPAEREGEEDDDGDVVR
                     RCTGGFGCPAQAKERLRHFASRNALDIEGLGDERIGLFFDEGLIREPADIFTLQRRDA
                     ASASPLAERKGFGKRSVENLFKAIEARRTVPLARLLFALGIRHIGETTANDLAKAFGT
                     FEAFKLAAERALEHQPGPAWRRFTAIPGLGEKTAENAMASVSAKAADLLAEDLFARPL
                     AERLVALKVVPPRAAGALAEAFGGDARALVRAANEAAASPPRDGFLELAGLSGIGIVA
                     TEALCDFQRSETNRSALDALMREIKVAPHERPVTEASAVTGKTVVFTGTLERLSRNEA
                     KAQAERLGAKVASSVSKKTDYVVAGAEAGSKLAKAEELGVKVLSEDDWIALIGGAS"
BASE COUNT          443 a          988 c         1008 g          434 t
ORIGIN      
        1 cgggtgagcc tttccggcgg ctgaccgtcc tcggcgtgac gagccgcata atgcgatcca
       61 ctatcgtcgg cgcgcttcgg atccggggcg cgccttttcc tttggcatcc gggagcagtt
      121 gccatgcgca agccgacgaa gagcgggcgc accgccatcg agacgccggc cgaagacctc
      181 gacgaggggg ccgcggccag ggaactcgag cgcctcgcgg gcctcatcgc gcatcacgac
      241 cggctctatc atcagcagga cgcgcccgag atcagcgacg cggagtatga tcggctccgc
      301 cagcgcaatg aattgattga gcagcggttt ccggcgctcg tgcgtgcgga cagcccgacg
      361 cagcgtgtgg gcgccacgcc ggccgaaagc ttcggcaagg tccgccatcg cgtgccgatg
      421 ctctcgctcg gcaatgcgtt cgcggaggcc gacgtcgtcg acttcctcgc ccggatcaat
      481 cgcttcctcg gtctcgaagc gtcggccgaa ctcgagttct ccgccgagcc gaagatcgac
      541 gggctctcga tctcgctgcg ctatgaggcg ggcgaactcg tcgaggctgc gacccgcggc
      601 gacggcgcgg agggcgagaa cgtcacggcc aacgtacgca cgatccggtc gatcccgcat
      661 aggctagctg gcaagcggca tccggccgtg atcgaggtgc gaggtgagat ctacctcagc
      721 cacgacgcct tccgcgcact caatgaggcg caggccgcgg cgggcgagaa ggtgttcgcc
      781 aatccgcgca atgcggccgc aggttcgctc cgtcagctcg atgcgagtgt caccgcacgc
      841 cgcccgctcg ccttcttcgc ctatgcctgg ggcgaggcga gcgcgctgcc gggcgacacg
      901 caagaggcga tctgccgttc cttcgaggcc tgggggctgc cggtcaacga gcggcgccgc
      961 gtcgtcaatg gggcggaggc gttgatcgcc ttctaccgcg agatcggcgc cgtgcgcgcc
     1021 tcgctcggct acgacatcga cggcgtcgtc tacaaagtga accgcctcga tctgcgcgag
     1081 cgcctcggct tcgtctcgcg cgcgccgcgc tgggcgatcg cgcacaagtt cccggccgag
     1141 caggcaacga ccattctcga agcgatcgac atccaggtcg gccgcaccgg agcgctgacg
     1201 ccggttgcca agctccgccc cgtcaccgtc ggcggcgtcg tcgtctcgaa tgcgtcgctc
     1261 cacaacgagg acgagatcgc gaggaaggac gtccgcatcg gcgacaccgt gatcgtgcag
     1321 cgcgccggcg acgtcattcc gcaggttctg ggagtggtgg gcgagaagcg gcctcctggc
     1381 acgatgccgt atgagttccc gcgcacctgc cccgcttgcg gcagcccggc cgagcgcgag
     1441 ggcgaggagg atgacgacgg cgatgtggtg cgccgctgca ccggcggctt cggctgcccg
     1501 gcgcaggcga aggagcggct ccgccatttc gcctcgcgca acgcgctcga catcgagggg
     1561 ctcggcgacg agcgtatcgg gctcttcttc gacgaaggcc tgatccggga gccggccgac
     1621 atcttcacgc tccagcgtcg tgatgcggcc tccgcgtcgc cgctcgctga acgcaaaggc
     1681 ttcggcaagc ggtcggtcga gaacctcttc aaggccatcg aggcgcggcg cacggtgccg
     1741 ctggcgcggc tgctgttcgc gctcggcatc cgccacatcg gcgagacgac ggccaatgac
     1801 ctcgctaagg ccttcggaac gttcgaggcc ttcaagttgg cggccgagcg cgccctcgag
     1861 catcagcctg gtccggcctg gcgccgcttc actgccattc cagggctcgg cgagaagacg
     1921 gccgagaacg ccatggcttc ggtaagcgcg aaggcggccg acctcttggc cgaggacctc
     1981 tttgcccgcc cgctcgccga gcggctcgtg gctctgaagg tcgtgccgcc gcgcgctgcc
     2041 ggcgcgctcg ccgaggcctt tgggggggac gcccgggcgc tcgtcagggc agcgaacgag
     2101 gcggcagcgt caccaccgcg cgacggtttc ctcgagctcg cggggctcag cggcatcggt
     2161 atcgtcgcga ccgaggcgct ctgcgacttc caacgctccg agacgaaccg gagtgcgctc
     2221 gacgcgctca tgcgtgagat caaggtcgcc cctcacgagc ggccggtgac ggaagcttcc
     2281 gctgtcacag gtaagaccgt cgttttcacc ggcacgctcg agcgcctctc gcgcaacgag
     2341 gcgaaggcgc aggcggagcg cctcggcgcc aaggtcgcaa gctctgtctc gaagaagacc
     2401 gactacgtcg tcgccggcgc ggaggcgggc tcgaagctcg ccaaggccga ggagttgggc
     2461 gtcaaggtgt tgagcgagga cgattggatc gcgctcatcg gcggcgcgtc gtgaggcgtg
     2521 ccgccatcgg cgcgacactc gcccaacact gccatcccgg aggccggagc gacccttccg
     2581 ccgcgaaggc gatgcgggac acagcgccat gagcagcgca gctttcgacc tcttcccacc
     2641 cgcaccaagg agcgctgcgc gcgcctcttg cgctgggtcc cggcgcggcg cgcccgcaag
     2701 agcgcgcgcc tggccgggat gatgatagtg ttgggctgcc gtcgcagggg agagttgtcc
     2761 ccgcgcgcga cggctgcgac tatcgtcgat gcacccaacg gtcgatcgac gacggtagcg
     2821 cgtgctgggc gtgatcggga ccgggggcga tgtcggacgg gccgtcctca gct
//
LOCUS       DAORNH010000413         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_26707_length_2837_cov_2.477354, whole genome shotgun sequence.
ACCESSION   DAORNH010000413 DAORNH010000000
VERSION     DAORNH010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26707_length_2837_cov_2.477354"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..410)
                     /locus_tag="PK264_24365"
     CDS             complement(180..410)
                     /locus_tag="PK264_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79028.1"
                     /translation="MKALSITIAIATLIALPANAQQIGGPNTGVGANVTLTPATPDRD
                     TRNRIGCRLGLVNPKVCASLNRNRVLRPGSNR"
     regulatory      585..697
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            747..956
                     /locus_tag="PK264_24370"
     CDS             747..956
                     /locus_tag="PK264_24370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79029.1"
                     /translation="MHVLLALISIIEVGLGLVVRLWQVGAAVRYGQLPILNLAIAVAS
                     LAAGGVGLNWLRRLWRRALVARSLE"
     gene            953..1312
                     /locus_tag="PK264_24375"
                     /pseudo
     CDS             953..1312
                     /locus_tag="PK264_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            1399..>2837
                     /gene="thiC"
                     /locus_tag="PK264_24380"
     CDS             1399..>2837
                     /gene="thiC"
                     /locus_tag="PK264_24380"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRD79030.1"
                     /translation="MNKIPSQSELMVPKVTTGPIVGSGKVYDSAEGHADVRVPFREIT
                     LSKEANEPPFRVYDSSGPYTDNSAKIDVEKGLPRHREAWVRERGGVEQYAGRDIKPED
                     NGNVRGKALARDFPNKTAPFRAHDGKPVTQYEFARAGIITKEMIYVAHRENLGRKAAL
                     ARAKAAIADGESFGAAIPEHITPEFVRDEIARGRAIIPANINHSELEPMIIGRNFLVK
                     INANIGNSAVTSSVEEEVEKMVWAIRWGADTVMDLSTGRNIHNTREWILRNSPVPIGT
                     VPIYQALEKCGGDPVKLTWELYRDTLIEQAEQGVDYFTIHAGVRLAYVPLTASRVTGI
                     VSRGGSIMAKWCLAHHRESFLYEHFDEICDLMRKYDVSFSLGDGLRPGSIADANDRAQ
                     FAELETLGELTQRAWKKGCQVMIEGPGHVPMHKIKINMDKQLKECGEAPFYTLGPLTT
                     DIAPGYDHITSGIGAAMIGWFGCAMLCYVT"
BASE COUNT          513 a          917 c          939 g          468 t
ORIGIN      
        1 ccccggccgg gacacacctc gacatcgagc tcgtcgacgg gcgcatcgca gcgaccgttg
       61 gcgaggggcc gcgagcaagt gcagatgcct cacccgctgc ggcaaggccc gcccgatccg
      121 gccgcccacc tgagcggcgc ggacccggcg ggcagggctc gctcttttga agtccgcctt
      181 cagcggttgc tgccgggcct taggacgcga ttgcggttga gcgaggcgca aaccttggga
      241 ttgaccaacc caagccggca gccgatgcgg ttgcgcgtgt cgcgatccgg cgtcgccggg
      301 gtgagcgtca cattggcgcc gacgcccgtg tttgggccgc cgatctgctg ggcattcgcg
      361 gggagagcaa tgagcgtggc aatggcgatc gtgatcgaga gggccttcat gacggtctcc
      421 ttcgtgtttc cgttcggctc ggtttgtcgt catccgatga ggagagattg cctgaagccg
      481 ctcgtccgcg ctgtgccgtg gagcaccggt caagcgcccg cagttccaca gggctggtcc
      541 gcatttcgag gcttgcacga ggcaacgtct tccaccaatg tgcagggagt tccgaggggg
      601 gccggcggga gccggctgag accatcccaa gtccgggtgg ccacccttag aacctgatcc
      661 gggtcatgcc ggcgaaggga cgggagcgac aaatgtccgg cactcgacat atccgaagct
      721 tgcttgccag ttcggtggga tcggtcttgc acgtgttgct tgcgctgatc tcgatcattg
      781 aggtggggct cgggctcgtc gtccggctct ggcaggttgg agcggcagtc cgctatggcc
      841 agctcccgat cttgaacctg gccattgcag tcgcctcgct cgcggccgga ggcgtcgggc
      901 tcaattggct ccggcggctg tggcgtcggg ctctggtcgc gaggagcctc gaatgacgct
      961 cgaagactac aacggctttt gccgctcgct tccgcacacg tcgcacgtgg tgcagtgggg
     1021 cggcgcgcac gtctggaagg tcgccggcaa ggtcttcgcc atcggctggt tcgagccggg
     1081 tgccgagccg caggtgactt tcaaagtgtc agagatgagc ttcgagatgc tgaaggcgac
     1141 gccgggctgc cggcccgcgc cctacctcgc ctcgcgcggc atgtcgtgga tccagcgctt
     1201 ctcgagcgaa agcatggacg atgcggggct tcgcgactat ctcgcagaga gccatcgcat
     1261 cgtcgcggca ggcctgccga agaagaggca gagggagctc ggcttcgcca cggcaccaga
     1321 tcgtccggcg ccccggacca aacacagatc actctccaac atccaacgac gatcgaccga
     1381 gtccacgagg aggacaccat gaacaagatc ccgagccagt ccgagctgat ggtgccgaag
     1441 gtgacgacgg gtccgatcgt cggctcaggc aaagtctacg acagcgccga ggggcatgcc
     1501 gacgtgcgcg tgccgttccg cgagatcacg ctctcgaagg aagcgaacga gccgccgttc
     1561 cgcgtctacg attcgtccgg tccctacacc gataactcgg ccaagatcga cgtcgagaag
     1621 ggcctgccgc gtcatcgcga ggcctgggtg cgtgagcgcg gcggcgtcga gcagtatgcc
     1681 ggccgcgata tcaagcccga ggacaacggc aacgtgcggg ggaaggcgct cgcccgcgac
     1741 ttcccaaaca agaccgcgcc cttccgcgca cacgacggca agccggtgac gcagtacgag
     1801 ttcgcgcgcg ccggcatcat caccaaggag atgatctacg tcgcccaccg cgagaacctc
     1861 ggccgcaagg cggcgctcgc acgcgccaag gcggcaatcg ccgacggcga gagcttcggc
     1921 gcggcgattc ccgagcacat cacgcccgag ttcgtacgcg acgagatcgc ccgtggccgc
     1981 gccatcatcc cggcaaacat caaccactcc gaactcgagc cgatgatcat cggccgcaac
     2041 ttcctcgtga aaatcaacgc caacatcggc aactccgccg tgacgtcgtc ggttgaggag
     2101 gaggtcgaga agatggtgtg ggcgatccgc tggggcgccg acacggtgat ggacctctcg
     2161 acgggccgca atattcacaa cacgcgcgag tggatcctgc gcaattcgcc ggtgccgatc
     2221 ggtaccgtgc cgatctacca ggcactggaa aagtgcggcg gcgatcccgt caagctcacc
     2281 tgggaactct atcgcgacac gctgatcgag caagcggagc agggtgtcga ctacttcacg
     2341 atccacgcgg gcgtgcgcct cgcctacgtg ccactcacgg ccagtcgcgt cacgggcatc
     2401 gtctcgcgcg gcggctcgat catggccaag tggtgcctcg cgcatcaccg cgagagcttt
     2461 ctctacgagc atttcgacga gatctgcgat ctcatgcgca agtacgacgt ctcgttctcg
     2521 ctcggcgacg gcttgcggcc gggctcgatc gcggatgcca atgatcgcgc ccagttcgcc
     2581 gaactcgaga cgctcggcga actcacgcag cgcgcctgga agaagggttg ccaggtgatg
     2641 atcgaaggcc ccggccacgt gccgatgcac aagatcaaga tcaatatgga caagcagttg
     2701 aaggagtgcg gcgaggcgcc gttctatacg ttgggcccgc tcacgaccga catcgcgccg
     2761 ggctacgacc acatcacgag cggcatcggc gccgccatga tcggctggtt cggctgcgcc
     2821 atgctctgct acgtgac
//
LOCUS       DAORNH010000414         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_27186_length_2798_cov_3.205250, whole genome shotgun sequence.
ACCESSION   DAORNH010000414 DAORNH010000000
VERSION     DAORNH010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27186_length_2798_cov_3.205250"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PK264_24385"
     CDS             complement(<1..473)
                     /locus_tag="PK264_24385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79031.1"
                     /translation="MSLARFALRTATVKAIRGRTFAGPFVRDSEIGPIDELASEGRIP
                     FVVVYTDDVALKCRGTDLLGNDGRTSLVMEIGVTTRMTPDGDWEIPTTDAGMELTIDA
                     IERQARTTLTAPAADNPWAELWREIVTDVAEIKSQRGASAREGVRFAGRQIVLEV"
     gene            complement(470..871)
                     /locus_tag="PK264_24390"
     CDS             complement(470..871)
                     /locus_tag="PK264_24390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79032.1"
                     /translation="MNRWADCLDRVDRAIDRVMAERLRIEPMAGGDFGGISPDPARSA
                     FEVDAVLSIERGETDLGGNAQKLRNVQIRGARAEAQIQRRLLPPGAELRKDDRLLALD
                     RGLAFKIERIDSEHPGRIALELSIDRSGATA"
     gene            complement(931..1962)
                     /locus_tag="PK264_24395"
     CDS             complement(931..1962)
                     /locus_tag="PK264_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HRD79033.1"
                     /translation="MPGIITLDIFNNDAFSLASLTARYNGETSMYVPGQIGRLGIFEE
                     GGVSTLTVMIEERDGSLSLIEPSKRGGPGETVGTNRDKLRSFVIPHLQRDDAVMADEV
                     QGRRAFGTDKDVETVQSVVDRKVNRHLRDLDATVEHHRVGAFKGVVLSKSGATLWNLY
                     NEFGIAEPAAVNFELDNAATNVRLKCEQLVREVEDELDTPYTGIHAMCGNDYWDKLTE
                     HKSVREVWLAAQKAAELLGKKADTIQIGGITFERYRTGKKATAGNGGAGFFAANEARF
                     VPKGVPELFITRFAPADYEETVNTDGLPRYVKQWAMPNGKGRNIEVQSNPLNICTRPA
                     VLRKGVVAA"
     gene            complement(2091..2462)
                     /locus_tag="PK264_24400"
     CDS             complement(2091..2462)
                     /locus_tag="PK264_24400"
                     /inference="COORDINATES: protein motif:HMM:NF014921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HRD79034.1"
                     /translation="MATLTEGRVTAAYILSESAGMRSRDHAKIASGAGKLEPGAILGK
                     VTASGKYVPHAPAAADGSQTAVAILYSAVDATAADAAAVITARDAEVKGAELIYNAAT
                     DTQPEKDAVHATLAANGIIVR"
     gene            complement(2607..>2798)
                     /locus_tag="PK264_24405"
     CDS             complement(2607..>2798)
                     /locus_tag="PK264_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79035.1"
                     /translation="MAADAAVTMLEKAPKAGQPSAAVAAPGADTMMGMELAAPVKEAR
                     KGPAPVDSRSVYERRRTAG"
BASE COUNT          359 a          915 c         1038 g          486 t
ORIGIN      
        1 tcgacctcga ggacgatctg gcgaccggcg aatcggacgc cctcgcgcgc cgaggcgccg
       61 cgctgcgatt tgatctcggc gacgtcggtg acgatctcgc gccagagctc ggcccaggga
      121 ttgtcggctg caggggctgt caaagtcgtg cgtgcctgac gctcgatcgc gtcgatcgtg
      181 agttccatgc cggcgtcggt ggtcgggatc tcccagtcgc cgtccggcgt catgcgcgtc
      241 gtgacgccga tctccatgac gaggctcgtg cggccgtcgt tgccgagcag gtcggtgccg
      301 cggcatttga gggcgacatc gtcggtgtag accaccacga acgggatgcg accctcggac
      361 gccagctcgt cgatggggcc gatctcgctg tcgcgcacga acgggccagc gaaggtccgg
      421 ccccggatgg ccttgacggt ggcggtgcgg agcgcgaagc gggcgaggct catgcggtgg
      481 ctccggagcg gtcgatcgaa agctcgaggg cgatgcggcc cgggtgctcg ctgtcgatgc
      541 gctcgatctt gaaggcgagc ccgcggtcga gtgccaggag ccggtcgtcc ttgcgcagct
      601 ccgcgccggg cggcagcagg cggcgctgga tctgtgcttc cgcgcgtgct cccctgatct
      661 gcacgttgcg gagcttctgc gcgttgccgc cgagatcggt ctcgccgcgc tcgatcgaga
      721 gcacggcgtc gacctcgaaa gccgaccggg cggggtccgg cgagatgccg ccgaagtcgc
      781 caccggccat gggctcgatg cgcaggcgct cggccatgac gcggtcgatg gcccggtcga
      841 cgcggtcgag acaatcggcc cagcggttca tgatgctctc gcggctccgg cgtcgggcgg
      901 cacgccgtgc atgccgccca tggctgatcg ttacgcggcg accacgccct tgcgcagcac
      961 ggcggggcgc gtgcagatgt tgagcgggtt cgactgcacc tcgatgttgc ggcccttgcc
     1021 gttcggcatg gcccactgct tcacgtagcg cggcaggccg tcggtgttga cggtctcctc
     1081 gtagtcggcc ggcgcgaagc gcgtgatgaa cagctcgggc acgcccttcg gcacgaagcg
     1141 cgcctcgttc gcggcgaaga agccggcgcc gccgttgccg gccgtcgcct tcttccctgt
     1201 gcgatagcgc tcgaaggtga tgccgccgat ctggatcgta tcggccttct tgccgagcag
     1261 ctcggccgcc ttctgcgccg cgagccacac ttcgcgaacg ctcttgtgct cggtgagctt
     1321 gtcccagtag tcgttgccgc acatggcgtg gatgccggtg tagggcgtgt cgagctcgtc
     1381 ctcgacttcg cgaacgagct gctcgcactt gaggcgcacg ttggtcgcgg cgttgtcgag
     1441 ttcgaagttg acggccgcgg gttcggcgat gccgaactcg ttgtagaggt tccagagcgt
     1501 cgcccccgac ttcgagagca cgacgccctt gaaggcaccg acgcggtgat gctcgaccgt
     1561 cgcgtcgagg tcgcgcagat gccggttcac cttgcgatcg accacgctct gcaccgtctc
     1621 gacgtccttg tcggtgccga aggcgcggcg gccctgcacc tcgtcggcca tcacggcatc
     1681 atcgcgctgg aggtgcggga tcacgaacga gcgcagcttg tcgcggttcg tgccgaccgt
     1741 ttcaccgggc ccgccgcgct tcgagggctc gatcagcgag agcgagccgt cgcgctcctc
     1801 gatcatgacg gtcagcgtcg agacgccgcc ctcctcgaag atgccgaggc ggccgatctg
     1861 gccgggaacg tacatggacg tctcgccgtt gtagcgggcc gtgagggagg cgagcgagaa
     1921 ggcgtcgttg ttgaagatgt cgagcgtgat gatgccgggc atgtgcgggg tctccttggc
     1981 cgggcggttg atcgggcttc aggccctctg tggaccgggg ccgctgtcac ggcccggcga
     2041 cgctaacagc agtcctggcg tcggctgccg gggcagccgt gcgccccgga tcagcggacg
     2101 atgatgccgt tggccgccag cgtggcgtgc accgcatcct tctcgggctg cgtgtccgtc
     2161 gcggcgttgt agatgagctc cgcgcccttc acctcggcat cgcgggccgt gatgacggct
     2221 gcggcatcag cggcggtcgc atcgacggcg gagtagagga tcgccactgc ggtctgtgag
     2281 ccgtcggccg cggccggcgc gtgcggcaca tacttgccgg acgccgtcac cttgccgagg
     2341 atggcgccgg gctcgagctt gccggcgccg gaggcgatct tggcgtggtc gcgcgagcgc
     2401 atgcccgcgg attcggacag gatgtaggcg gccgtcacgc ggccttcggt cagcgttgcc
     2461 atcaggggtc tccgtgatga tggatagaag gctcggggat ggcacgcgcc cggcgagacg
     2521 gacccggagg gccggcgcgg ggctcacatc ttgcccaagg ggccatcccc gacgagggct
     2581 ctctcgcggg gtattccggt ggtggttcag ccggcggtgc ggcggcgctc gtagacgctg
     2641 cggctgtcga cgggcgcggg gcctttgcgc gcctccttga cgggggccgc gagctccatg
     2701 cccatcatcg tgtcggcgcc gggggcggcg accgcggccg agggctggcc ggccttcggc
     2761 gccttctcga gcatggtgac ggcagcgtcg gccgccat
//
LOCUS       DAORNH010000415         2749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_27838_length_2749_cov_2.080178, whole genome shotgun sequence.
ACCESSION   DAORNH010000415 DAORNH010000000
VERSION     DAORNH010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27838_length_2749_cov_2.080178"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>362)
                     /locus_tag="PK264_24410"
     CDS             complement(<1..>362)
                     /locus_tag="PK264_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRD79036.1"
                     /translation="SRITERFGRPSCVIAWEGDRGTGSLRSIAGVDIGSAVRAAAEGG
                     LLIKGGGHAMAAGLTVAKARADEARAYIAAAVKASAVTARAASGLDIDGALTPGAATA
                     ELIDLIEPPAAERPCARL"
     assembly_gap    363..406
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<407..1567)
                     /locus_tag="PK264_24415"
     CDS             complement(<407..1567)
                     /locus_tag="PK264_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRD79037.1"
                     /translation="MSAQRRYAARDAEADGLPLAFLGVTRSARGLAWRERLSAEQRNV
                     ALAISQRQGLPDVLGRILGARGYGPDDIETALDPTIKALMPDPSSLQDMDKAAARIAD
                     AIVAGEQIAIFGDYDVDGACSSALLHRFLTHHGLAPRIYIPDRMLEGYGPNPAAIEQL
                     AKAGSRLIVTVDCGTTSHDALAVAARNKSAVVVIDHHQADERLPAVHAVVNPNRQDDL
                     SALGHLCAAGVVFMTLVAVSRELRRRGHYGAKCTPPDLLALLDLVALATVCDVVPLVG
                     LNRAFVRKGLQVMHQRHHPGIRALFDAAGLDRPPTPYTLGFVLGPRINAGGRIGDAGL
                     GAKLLSTDDEVEAARIAVLLDKLNRERKALETRILEEAIADADRQVDADPELP"
     gene            complement(1687..>2389)
                     /locus_tag="PK264_24420"
     CDS             complement(1687..>2389)
                     /locus_tag="PK264_24420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRD79038.1"
                     /translation="MPRLGAGVVRTTQPLSQLARSFLLLGSTFMFFNGVKHLELAQAA
                     SISFMAPFIVVLLALPMLGEPISIHRIGAVLAGFVGVLIVIRPGSAVFQWASLLIVGS
                     ACCYALYQIFTRRVAGHDRPETSAFYSALIGTLVMAGVITWKGFAPPKSLLDAGLLAS
                     LGILGAAGHYCVARAMTYAAANVVAPFQYWQMVGSVIVGYLVSHRLPDAYTWLGASVI
                     IGAGLYIGWRDARRV"
     assembly_gap    2390..2409
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          435 a          895 c          972 g          383 t
ORIGIN      
        1 acaatcgtgc gcagggtcgc tccgcggccg gcggctcgat gagatcgatg agttccgccg
       61 tggcagcacc cggcgtcagc gcgccgtcga tgtcgagccc ggaggcggcg cgggccgtga
      121 ccgcactcgc cttcacggcc gcggcgatgt aggcgcgcgc ctcgtccgcc ctggccttgg
      181 caaccgtgag gcccgcggcc atggcgtggc cgccgccttt gatgaggagc ccgccttcgg
      241 ccgcggcgcg caccgccgat ccgatgtcga cacccgcgat ggagcgcagc gagcccgtgc
      301 cgcggtcacc ctcccaggcg atcacgcacg acgggcggcc gaagcgctcc gtgatgcggc
      361 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncggc agctcgggat
      421 cggcatcgac ctggcggtcg gcgtcggcga tcgcctcctc gaggatgcgc gtctcgaggg
      481 ccttcctctc tctattgagc ttgtcgagca ggacggcgat gcgcgcggcc tccacctcat
      541 cgtcggtgga gagcagcttc gccccgagcc ccgcgtcacc gatccgcccg ccggcattga
      601 tgcgcggccc gagcacgaaa ccgagcgtgt agggggtcgg cggacggtcg aggccggccg
      661 catcgaatag cgcgcggatg cctggatgat gccgctggtg catcacctgc aggcccttcc
      721 ggacaaaggc ccggttgagg ccaaccaacg gaacgacgtc gcagaccgtc gcaagcgcca
      781 cgagatcgag gagggccagc aggtcgggcg gcgtgcattt cgccccgtag tgaccgcggc
      841 ggcgcagttc gcgcgagacc gcaaccagcg tcatgaagac gacgccggcc gcgcagaggt
      901 ggccgagcgc ggagagatca tcctggcggt tgggattgac gacggcatgc accgccggca
      961 gacgctcgtc ggcctggtga tggtcgatca caacgacggc ggatttgttg cgtgccgcca
     1021 cggccagcgc atcgtggctc gtcgtgccgc agtcgacggt gacgatgagg cggcttcccg
     1081 ccttcgcgag ctgctcgatc gccgccgggt tcggcccgta gccttcgagc atgcggtcgg
     1141 ggatgtagat gcgcggggcg aggccgtggt gggtgaggaa gcgatgcagc agcgccgacg
     1201 agcaggcgcc gtccacatcg taatcgccga agatcgcgat ctgctcgcct gccacgatgg
     1261 catcggcgat gcgggccgcc gccttgtcca tgtcctggag cgagctcggg tccggcatca
     1321 gcgccttgat ggtcgggtcg agcgcggtct cgatatcgtc cgggccgtag ccgcgcgcgc
     1381 cgagaatgcg gccgagcacg tccggcaatc cttggcgctg cgagatggcg agcgccacgt
     1441 tgcgctgctc cgccgagagc cgctcgcgcc aggcgaggcc gcgcgccgac cgggtgacgc
     1501 cgagaaacgc gagcggcagg ccatcggcct cggcatcgcg tgcagcgtag cgccgttgtg
     1561 cggacacgga cgtctccctt cgctatcgca gatgcagcga ctcagcctcg cgtacttcaa
     1621 gcacgccctg tctgcctcgg aaaggcgggt tgcagccgct ccccaggaat tgccccgagt
     1681 gggccctcag acgcggcgcg cgtcgcgcca gccgatatag agcccggcgc caatgatgac
     1741 ggacgcgccg agccaagtgt aggcgtcggg gaggcggtgc gagacgaggt agcccacgat
     1801 caccgagccg accatctgcc agtactggaa cggggcgacg acattggcgg cggcataggt
     1861 catggcgcga gcgacgcagt agtggccggc cgcgccgaga atgccgaggc tcgcgaggag
     1921 gcccgcgtcc agcagcgact tcggcggcgc aaagcccttc caggtgatga cgcccgccat
     1981 gacgagggtg ccgatcagcg cactgtagaa ggcgctggtc tccggccggt catggcccgc
     2041 cacgcgacgt gtgaagatct gatagagcgc atagcagcag gcgctgccga cgatcagcag
     2101 cgaggcccac tggaagacgg ccgagcccgg acgtatcacg atcagcacgc cgacgaagcc
     2161 cgccagcacc gctccgatgc gatggatcga gatcggctcg ccgagcatgg gcagcgccag
     2221 cagcacgacg atgaaaggtg ccatgaagct gatggaggcc gcctgggcga gctccaggtg
     2281 cttcacgcca ttgaagaaca tgaaggtcga gccgagcagc aggaacgagc gcgcgagctg
     2341 cgagagcggc tgtgtcgtgc gcacgacgcc ggccccgagc cgcggcatgn nnnnnnnnnn
     2401 nnnnnnnnng cggtgcgcca gaacacgatc tgctcgctgt cgtagcggac gctcagcacc
     2461 tgcacgagcc cgttcatgat cgggaacagc gtgccggcaa tgcacatgaa gagaatgccg
     2521 agcagcggcc gcgagggtgg cttggtggcg gcaccggatg cggtcggtgg ggcggtcatg
     2581 atccggtgct tgtctcgtct ctcgtatccg cggcatctga tcggcatgcc gccatcatgc
     2641 agcctcagcg gcgctgctcc tgtgctttgc gcagagctgc cgcgaacgcg ctctcgccgg
     2701 cggtcggctg ggcgccgccg cccgacgcgc gtggtggtgc tgcagacat
//
LOCUS       DAORNH010000416         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_27973_length_2739_cov_2.519374, whole genome shotgun sequence.
ACCESSION   DAORNH010000416 DAORNH010000000
VERSION     DAORNH010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27973_length_2739_cov_2.519374"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK264_24425"
     CDS             <1..310
                     /locus_tag="PK264_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRD79039.1"
                     /translation="TLSHGAQRQLEIAMTLAIEPKVLLLDEPLAGMGQEEGERMAALL
                     RTLAEDHAVLLIEHDMDFVFKVADRMTVLVEGAVLASGTPAAVRADTAVQHAYLGGHA
                     "
     gene            307..1023
                     /locus_tag="PK264_24430"
     CDS             307..1023
                     /locus_tag="PK264_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRD79040.1"
                     /translation="MTRDALIDARDLHTYYDESHILRGVSLSLRAGETLGLMGRNGMG
                     KSTLIRTIMGLVRPRSGSVAIAGRDMTRAAPFRIAQSGIAYVPEGRGIFASLTVAENL
                     AIAERAGVDGGTAWTRARILDLFPRLSQRFTNRGDQLSGGEQQMLAIGRALLTNPRVL
                     ILDEATEGLAPLIRDEIWRTVRLVRDSGIATLVVDKTVSEVTAVADRVVILVKGLIVF
                     EGTPADLKASPEVMMQHLGV"
     gene            1105..2673
                     /locus_tag="PK264_24435"
     CDS             1105..2673
                     /locus_tag="PK264_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HRD79041.1"
                     /translation="MPTTLWSPPTGAYNAAVDMVDRHLAEGRGDKVAFIDPTRRLTYA
                     ELAAGCNRFANALPRLGIERERRIVQIMLDTVDFPIVFWGAIKAGVVPIPLNTLLPNE
                     TWAYMIEDSRADAVVISAELFERARPMLTEIGGRRHLHVIVTGSGGDGTPLSLEKLLA
                     TSGDEAKAADTNADEVAFWLYSSGSTGMPKGARHLHTSPMATAKLFGQGIVGIGADDI
                     IFSAAKLFFAYGLGNGMSFPLSVSATTILLPDRPTPDSVLAVMKAHNPTIFCGVPTLY
                     ASMLASPALGKGAASNRLRISTSAGEALPEEIGNRWQQVVGSEIIDGIGSTEMLHIFV
                     SNRPGKVRYGSSGTPVPGYEAKLLDDDGSPVAKGEIGELVIRGPSAADGYWLQRQKSL
                     KTFRGEWTHTGDKYRIGEDGCYYYCGRTDDMFKVSGIWVSPFEVESALITHKAILEAA
                     VIGKEDTDGLVKPKAFVILKPGVAADEALFEDLKAHVKATAGAWKYPRWVEARTELPK
                     TATGKIQRFKLRAE"
BASE COUNT          469 a          924 c          911 g          435 t
ORIGIN      
        1 cacgctcagc cacggcgcgc agcgccagct cgagatcgcc atgacgctgg cgatcgagcc
       61 caaggtgctg ctgctcgacg agccactcgc cggaatgggc caggaggagg gcgagcgcat
      121 ggccgcgctg ctgcgcacgc tcgccgagga tcacgcggtg ctgctcatcg agcacgacat
      181 ggatttcgtc ttcaaggtcg ccgatcgcat gacggtgctc gtcgaaggcg ccgtgcttgc
      241 ctcgggtact cccgccgcgg tgcgcgccga tacggccgtg cagcacgcct atctcggggg
      301 gcatgcatga cccgcgacgc gctcatcgat gcccgcgacc tccacaccta ctacgacgag
      361 agccacatcc tgcgcggcgt ctcgctcagt ttgcgggccg gcgagacgct aggcctcatg
      421 ggccgcaacg gcatgggaaa gtcgacgctg atccgcacca tcatgggcct cgtccgtccg
      481 cgctcgggca gcgtcgccat cgccggccgc gacatgacgc gcgcagcgcc gttcaggatc
      541 gcccagagcg gcatcgccta cgttcccgag ggccgcggca tcttcgcctc cttgaccgtt
      601 gccgagaacc tcgccatcgc cgagcgtgcc ggcgtcgacg gcgggacggc ttggacgcgg
      661 gcgcgcattc tcgatctctt cccgcggctc agtcagcgtt tcaccaatcg cggcgaccag
      721 ctctcgggcg gagagcagca gatgctggcg atcggccgcg cgctcctcac gaacccgcgc
      781 gtgttgatcc tcgacgaggc gaccgaaggg cttgcgccgc tcatccgcga cgagatctgg
      841 cgcaccgttc gcctcgtgcg cgacagcggc atcgccacgc tcgtcgtcga caagaccgtg
      901 tccgaggtga ccgccgtcgc ggaccgagta gtgatcctcg tcaaggggtt gatcgtgttt
      961 gaagggaccc cggccgacct caaggcgtcg cccgaggtga tgatgcagca cctcggcgtg
     1021 tgaggtgttg ccccgaggcg cgcgttcggg cgcagccatc atggaaatgc agtcagtact
     1081 gccagcgagc gcagggagga acacatgccg accacactct ggtctccgcc gacgggcgcc
     1141 tacaacgcag ccgtcgacat ggtggatcgc catctcgcgg agggtcgcgg ggacaaggtt
     1201 gcgttcatcg atccgacgcg gcggctcacg tacgccgagc tcgcggccgg ctgcaaccgc
     1261 ttcgccaatg cgctgccgcg gctcggcatc gagcgggagc ggcgcatcgt gcagatcatg
     1321 ctggacacgg tcgatttccc gatcgtgttc tggggagcga tcaaggcggg cgtggtgccc
     1381 atcccgctca atacgctgct gcccaatgag acatgggcct acatgatcga ggatagtcgc
     1441 gcggacgccg tcgtcatctc cgccgagctc ttcgagcgcg cgcggccgat gctgaccgag
     1501 atcggtggcc ggcggcacct gcatgtcatc gtgacgggga gcggcggaga tgggacgccg
     1561 ctctcgctcg aaaagctgct cgcgacgagc ggcgacgaag ccaaggccgc cgacacgaac
     1621 gccgacgagg tcgccttctg gctctactcg tcgggatcga ccggcatgcc gaaaggcgcg
     1681 cggcacctgc acacgagccc gatggcaacc gccaagctct tcggacaggg tatcgtcggc
     1741 atcggcgccg acgacatcat cttctcggcc gcgaagctct tcttcgccta cggcctcggc
     1801 aacggcatgt cgtttccgct ctcggtcagc gcgaccacca ttctactccc cgaccgcccg
     1861 acgcccgaca gcgttctcgc cgtcatgaag gcgcacaatc cgacgatctt ctgcggcgtg
     1921 ccgaccctct acgcttcgat gctggcctcg cccgcgctcg gcaagggtgc cgcctcgaac
     1981 aggctgcgca tctcgacatc ggcgggcgag gcgctgcccg aggagatcgg caatcgctgg
     2041 cagcaggtcg tcggttccga gatcatcgac ggcatcggct cgacggagat gttgcacatc
     2101 ttcgtatcta accggccggg gaaggtgcgc tacggctcgt ccggcacgcc agtccccggc
     2161 tacgaggcga agctgctcga cgacgacggc agcccggtcg ccaagggcga aatcggcgaa
     2221 ctcgtgatcc gcggcccctc cgcggccgac ggctactggc tgcagcggca gaagtcgctg
     2281 aagacgtttc ggggcgagtg gacgcacacc ggtgacaagt atcgcatcgg cgaggacggc
     2341 tgctactact actgcgggcg cacggacgac atgttcaagg tatccggcat ctgggtctcg
     2401 cccttcgagg tcgagagcgc gctcatcacg cacaaggcca tcctcgaggc ggccgtgata
     2461 ggcaaggagg atactgatgg cctggtgaag cccaaggcct tcgtcattct gaagccgggc
     2521 gttgcggcgg acgaggcgct cttcgaggac ctcaaggcgc acgtcaaggc aacagccggc
     2581 gcctggaaat acccgcgctg ggtcgaggcg cgcaccgagt tgccgaagac ggcgaccggc
     2641 aagatccagc gtttcaagct cagggcggaa tgaagggcgg ctcggcaacg atgaagcgac
     2701 ttgtgttcga ggacgacaag ctcgtccgct tccagcatt
//
LOCUS       DAORNH010000417         2719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_28273_length_2719_cov_2.593468, whole genome shotgun sequence.
ACCESSION   DAORNH010000417 DAORNH010000000
VERSION     DAORNH010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28273_length_2719_cov_2.593468"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1016
                     /locus_tag="PK264_24440"
     CDS             402..1016
                     /locus_tag="PK264_24440"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HRD79042.1"
                     /translation="MAEAFRLLEQADRGHAMTLRRSLRDGTGELHSRLDRLVERNGHF
                     DSLEGYSRWLIAMHAFYGETALALSNLEADDIVDQQAWTARLCRLSADLTDLSATIPP
                     PPAETDLEIHDEVDALGLLYVTEGASLGARLLVRRVRALGCSENYGARHLSAEARGNA
                     AWQRISAIFEARELDSEASARLVSMSRRVFSLAAASLGDRHDDP"
     gene            1003..>2015
                     /locus_tag="PK264_24445"
     CDS             1003..>2015
                     /locus_tag="PK264_24445"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRD79043.1"
                     /translation="MTTRDASVDLAHCDREPIHIPGLVQSFGCLIACRSDSWTVSHVS
                     ANCTAFGLDAPLVLGQPFKAAVGREASHEVANALSVSMAPGLPGRLFDVLLASGQRCD
                     IAVHSHAGRIIVELEPMQTETDASTPLVIVRSMLARMQDAADVDSLCAIAVKQIRALI
                     NFDRVMIYRFLQDGSGCVIAESRSDDVPSFLEHHYPASDIPRQARELYVKSWLRLISN
                     VHAVPVPLLVAASDATVPLDLTYSTLRSVSPVHIEYLRNMQVGASLSISIVVGGALWG
                     LIACHNRTARVVPPDIRVTAEFFGQAFSLQLQTLARADAAEMLRQARDRIHQIVSELP
                     PDEP"
     assembly_gap    2016..2025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2026..>2719
                     /locus_tag="PK264_24450"
     CDS             <2026..>2719
                     /locus_tag="PK264_24450"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HRD79044.1"
                     /translation="LTRSLGSRLDEIAAILPCDGAALWLDGELCSWGECPPRDEFPAI
                     VSKLDSVVEGAVLATHELAKLHPPAERFAGEASGLLALPLSRNAPNFLLLFRREFVRT
                     ISWAGNPTKPFETGPIGTRLTPRKSFELWREEVKNQSRPWSPQDRLTAEALRTSLLEI
                     VLKYSEIVAVERSRADQLQRIQNAEFNHRVKNALALIGALVSQSKAGHETLSSFVADL
                     EGRGPEMPGRADR"
BASE COUNT          460 a          914 c          835 g          500 t
ORIGIN      
        1 gacgaacttc gcgatcgata aggacactcg caatgtcatg agcttagtgt ggcgttatgt
       61 tcggaagttc gtatttcgag tgtgccgcac agtgaatacg aacttccgaa ccgccacact
      121 aggtcgcccc catctcgtag gcgccgcgct cgcaaccctg cgcgagtcgc ttcggctcga
      181 ggacttggcg atctgtggat cggtacatga gggcgccgat cagaacggac catcgaatga
      241 cctcgtttgg cccgtgggtt caatcgtctc gccaatcgac ttgggctcgt gcccacggcc
      301 cccgcttggc atctcgagcc gattttacct gcgtcgcggt cgaggatcgc ctaggatcat
      361 gttcggcaac ggcgcacgat gggcccggcg cggcggccga gttggccgaa gcgtttcgcc
      421 tgctcgagca ggccgatcga gggcacgcca tgacattgcg acgcagcttg cgagacggga
      481 ccggtgagct gcacagcagg ctcgatcgac tggtcgagcg taacggccat ttcgattcgc
      541 tcgagggcta ctctcgctgg cttatcgcaa tgcatgcgtt ctacggggag accgccctcg
      601 cgctcagcaa tctcgaagcc gatgatatcg tcgatcagca agcctggacc gcgcgattgt
      661 gccgactgtc cgccgacttg accgatctat cggcgacgat cccgcccccc cccgccgaaa
      721 cagatctcga gatccatgac gaagtcgatg cgctcgggct cctctatgtc actgaggggg
      781 cctcgctcgg cgcccggctg cttgtcaggc gcgttcgtgc cctcggctgc agtgagaact
      841 acggcgcacg acacctcagc gccgaagcgc gtggcaatgc ggcgtggcaa cggatcagcg
      901 cgattttcga ggcccgcgag cttgacagcg aggcctccgc acgactggtc agcatgagcc
      961 gacgcgtatt ctcgctggca gcggctagtc ttggagaccg ccatgacgac ccgtgacgcc
     1021 agtgtcgacc tcgctcactg cgaccgcgag ccgatccaca tccccggctt ggtgcaatcg
     1081 ttcggttgcc tcatcgcgtg ccgaagcgac agctggactg tatcccatgt ctctgccaac
     1141 tgcaccgcgt tcggcctcga tgccccgctc gtcctcgggc agccattcaa ggccgcggtc
     1201 ggtcgggagg cgtcgcacga ggtggctaac gcgctctcgg tatcgatggc tccggggctg
     1261 cccggtcgcc tcttcgatgt ccttctggcg agcggacagc ggtgcgatat cgccgtccac
     1321 agccatgccg gcaggatcat cgtcgagctc gagccaatgc aaacggagac cgacgcgtcg
     1381 acaccgctcg tcatcgtgcg cagcatgctg gcccgcatgc aggacgcggc agacgtcgac
     1441 agtctctgtg ccatcgcggt gaagcagatc cgcgcgctca tcaacttcga tcgcgtgatg
     1501 atctaccgct tcctccagga cggctccggc tgcgtcatcg ccgaaagccg ctccgacgat
     1561 gtgccgtcct tcctcgagca tcactatccg gcctccgaca tcccgaggca ggcacgtgaa
     1621 ctctatgtca agagctggct acggctgatt tccaacgttc acgccgttcc tgtcccgctg
     1681 ctcgtcgccg cgagcgacgc aacggtgccg ctcgacctca cctacagcac gctgcgcagc
     1741 gtctcgccgg tgcatatcga gtacctccgg aatatgcagg tcggtgcttc gctgtcgatt
     1801 tcgatcgtgg tcggcggcgc gctctggggg ctgatcgcat gtcacaatcg caccgcgcgt
     1861 gtggtccctc ctgacatccg cgtgaccgcg gaattctttg gccaggcctt ctcgctccag
     1921 cttcagaccc ttgctcgcgc ggacgcggca gagatgctgc ggcaggcgcg cgaccgcatc
     1981 caccagattg tgtccgagct gccgccggac gagccnnnnn nnnnngctga cccgcagcct
     2041 cggctcgcgg ctcgatgaaa tcgcggcgat cttgccgtgc gacggggcag cactctggct
     2101 cgacggcgag ctctgctcgt ggggcgagtg cccgccgaga gatgagttcc cggccatcgt
     2161 ctccaagctc gacagcgtcg tagaaggggc tgtgctggca acgcacgagc tggcgaaact
     2221 ccatcccccc gccgagcggt ttgcgggcga ggcgagcggg ctcctggccc tgccactgtc
     2281 gagaaatgcg ccgaacttcc tgttgttgtt ccgccgcgag ttcgtcagga ccatcagctg
     2341 ggcgggcaac ccgaccaagc cgtttgagac cgggccgatc ggaacgcgcc tcacgccgcg
     2401 caagagcttc gaactctggc gcgaggaggt caagaaccag tcgcgcccgt ggagcccgca
     2461 agaccggctc acggccgagg cgcttcgcac ctcgctcctc gaaatcgttc tcaagtacag
     2521 cgagatcgtc gccgtcgagc gctcccgtgc cgaccagctg caacgcatcc agaatgccga
     2581 gttcaatcat cgggtcaaaa acgcgctcgc ccttattggc gcgctcgtct cgcagagcaa
     2641 ggccggccac gaaacgctgt cctccttcgt tgccgatctc gaagggcgcg gcccagaaat
     2701 gccgggtcga gccgaccgt
//
LOCUS       DAORNH010000418         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_28425_length_2708_cov_2.763664, whole genome shotgun sequence.
ACCESSION   DAORNH010000418 DAORNH010000000
VERSION     DAORNH010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28425_length_2708_cov_2.763664"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..708
                     /locus_tag="PK264_24455"
     CDS             157..708
                     /locus_tag="PK264_24455"
                     /inference="COORDINATES: protein motif:HMM:NF023985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRD79045.1"
                     /translation="MTERVLTAKITRAGMRALFNATNTGVQLDLSHMAIGNGIANAGY
                     TPNGNEVALGGEFARVEIGSGDYLGDYEIMVTGLFSDATAGWANEVGIFTSTGVLFAV
                     WSEPAAPIAYKTANVPFVLALTLALGDVPPGSINIIVGAPSVNIMIVGPFAVISAEII
                     RLQRRMVESENARLTPIINSTWQ"
     gene            746..1528
                     /locus_tag="PK264_24460"
     CDS             746..1528
                     /locus_tag="PK264_24460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79046.1"
                     /translation="MSLETQIAALTQQAGLLLDLPQQIATTATAKIAEVTAAYNQRMA
                     TFTVTVTVDEVTGLDTQPGTDALPVRTMAKALSMVPVGGVGTIRLASDVTLTATIRIA
                     NKRVVIVSNSAVRRKLSFAKLPWIAGASTYRGVAAFGLADGASLHFNFVELMIPDGAG
                     DAHIPHWFASPIGVSVTPGAGAPDVGFYDVAINLPATPYAAVIGVGFGSTLHRPTSLW
                     VYAMTLPGAITSINGRLFHDITATAGTAAASLNWLTTNLTTV"
     gene            1578..2006
                     /locus_tag="PK264_24465"
     CDS             1578..2006
                     /locus_tag="PK264_24465"
                     /inference="COORDINATES: protein motif:HMM:NF025659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HRD79047.1"
                     /translation="MLLTLSHAGVGYVNATPEQLQAAGVPASAWLKPMLKAYAADKRW
                     RIEIGGIVVNGAPVPTDDRAKALLTGAAASMTSTETTKVKVGGAFVDMTGTQVKALRD
                     AVADHVQACFQAEADVVAEIDAGTITTLAEVDAFAWPANA"
     gene            2066..>2708
                     /locus_tag="PK264_24470"
     CDS             2066..>2708
                     /locus_tag="PK264_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HRD79048.1"
                     /translation="MADISFHHGTRVFESQETPVLLRTAQSAVVALIGTAPNADAAAF
                     PLNTPVLLLGSTQLSKVEKLGDAGTLKKAVDGVFDQVGTYCYIIRVAEGVSLQATWSN
                     LVGSAAALTGVHALKKCEGLFGRKLKPRLVAVPGYTSTTAADGIASVAVTAQGGGYTT
                     VPSVTVTGGGGMGCEAKAIVESGAVTAVVVTKPGFGYTGAPVVTIAGGGGAGAT"
BASE COUNT          450 a         1015 c          841 g          402 t
ORIGIN      
        1 gacccgcttc tcggccgcgg cgcgcgctcg cggcctcacc atcagcatca acggcgcagc
       61 ggtcggaccg gcaaagacgc ccgtcgcccg gctcgccgca gcggtccgcg cccgcgccct
      121 caccgtcgca agctttcgca tggaggccag actgtaatga ccgagcgcgt gctcacagcc
      181 aagatcaccc gcgcaggcat gcgcgccctc ttcaatgcca ccaacacggg cgtccagctc
      241 gacctctcgc acatggcgat cggcaacggc atcgcgaatg ctggttacac accgaacggc
      301 aacgaggtcg cactcggcgg cgagtttgcg cgcgtcgaaa tcggcagcgg cgactatctc
      361 ggcgactacg agatcatggt gacggggctc ttctccgacg ccaccgcggg ctgggccaac
      421 gaggtcggca tcttcacctc gaccggcgtc ctcttcgcgg tctggagcga gcccgccgca
      481 ccgatcgcct acaagacggc caacgtcccc ttcgtactgg cactcacgct ggcgctcggc
      541 gatgtgccgc ccggctcgat caacatcatc gtcggcgcgc cgagcgtcaa catcatgatc
      601 gttggcccgt tcgccgtcat ctcggcggag atcatccgcc tgcagcgccg catggtcgag
      661 agcgagaacg cacgcctcac cccgatcatc aactcgacct ggcagtgatc gccaccccag
      721 accgacctga acacggagac cccggatgag cctcgaaacc cagattgccg cactcacaca
      781 gcaggccggc ctcctgctcg atctgccgca gcagatcgcc acgaccgcga ccgccaagat
      841 cgccgaggtg actgcggcct acaatcagcg catggcgacc ttcaccgtga ccgtcacggt
      901 cgacgaggtg accggcctcg acacgcagcc gggcacggac gctttgccgg tccgcaccat
      961 ggcgaaagcc ctctcgatgg tgccggtcgg cggcgtcggc accatccgac tcgcctcgga
     1021 cgtgacgctc accgcgacga tccgcatcgc caacaagcgg gtcgtgatcg tctcgaattc
     1081 ggccgttcgc cgcaagctca gcttcgcgaa gttgccctgg atcgccggtg cctcgactta
     1141 tcgcggcgtc gcagcgttcg gcctcgctga cggcgccagc ctgcacttca acttcgtcga
     1201 gctgatgatc ccggatgggg cgggcgacgc ccacatcccg cactggttcg cctccccgat
     1261 cggcgtctcg gtcacgcccg gcgcgggtgc gccggacgtc ggcttctacg acgtggcgat
     1321 caatctcccg gcgacgccct atgccgccgt gatcggcgtc ggcttcggca gcaccctgca
     1381 tcggccgacg tcgctctggg tctacgccat gacgctcccc ggggccatca catcgatcaa
     1441 cggacgcctc ttccacgaca tcacggcgac cgccggcacg gcggcggcca gcctcaactg
     1501 gctgacgacc aacctcacca ccgtctgatc cggcgccgac cggaggcgcg ctcaactgca
     1561 atcgaggatc cctcacaatg ctactcaccc tctcgcacgc cggcgtcggc tacgtgaacg
     1621 ccacgccgga gcagctccag gcggccggcg tgcctgcgtc cgcatggctc aagcccatgc
     1681 tgaaagccta cgccgccgac aagcgctggc ggatcgagat cggcggcatc gtcgtgaacg
     1741 gcgcgccggt cccgaccgac gatcgcgcca aggcgctgct caccggagcg gccgcctcga
     1801 tgacatcgac cgagaccaca aaggtgaagg tcggcggcgc cttcgtcgac atgacgggca
     1861 cgcaggtcaa agcgctgcgc gatgccgtcg ccgaccacgt gcaggcctgc ttccaggccg
     1921 aggccgacgt ggtcgccgag atcgacgccg gcacgatcac gacactcgcc gaggtcgacg
     1981 ccttcgcctg gccggcgaac gcctgacgat ccgcaattcc tagcgaccct ccgccgcgag
     2041 ccgcggccaa ccctggagcc ctcccttggc agacatctcc ttccatcacg gcacgcgcgt
     2101 cttcgagagc caggagacgc ccgtgctgct gcgcacagcg cagtccgccg tggtcgccct
     2161 gatcggcacg gctccgaatg ccgatgccgc ggcgtttccg ctcaacacgc cagtgctgct
     2221 gctcggctcg acgcagcttt ccaaagtcga gaaactcggc gacgccggca ccctcaaaaa
     2281 ggccgtcgac ggcgtcttcg accaggtcgg cacctactgt tacatcatcc gcgtcgccga
     2341 aggcgtctcg ctgcaggcca cctggtcgaa cctcgtcggc tcggcggcgg ccctcacggg
     2401 cgtgcacgcg ctcaagaagt gcgaagggct gttcgggcgc aagctcaagc cgcgcctcgt
     2461 cgccgtgcct ggatacacct cgacgacggc ggccgacggc atcgcctctg tcgccgtcac
     2521 ggcgcagggt ggcggctaca cgaccgtacc gtccgtgacg gtgacgggcg gtggcggtat
     2581 gggctgcgag gcgaaggcga tcgtcgagag cggcgctgtc acggcagtcg tcgtcaccaa
     2641 gccgggcttc ggctacacgg gcgcgcccgt cgtcacgatc gctggcggtg gcggcgcggg
     2701 cgccaccg
//
LOCUS       DAORNH010000419         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_28762_length_2682_cov_2.422916, whole genome shotgun sequence.
ACCESSION   DAORNH010000419 DAORNH010000000
VERSION     DAORNH010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28762_length_2682_cov_2.422916"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1331)
                     /locus_tag="PK264_24475"
     CDS             complement(<1..>1331)
                     /locus_tag="PK264_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRD79049.1"
                     /translation="IEADTEEARAAAKRPLDVIEGPLMSGMNVVGDLFGAGKMFLPQV
                     VKSARVMKQAVAYLQPFLEAEKKATGLDLRPAAGKVIMATVKGDVHDIGKNIVGVVLQ
                     CNNYEVIDLGVMVPAAKVLETAKRENADVIGLSGLITPSLDEMCHVAAEMEREGFSVP
                     LLIGGATTSRVHTAVKISPNYKRGQAIYVTDASRAVGVVSSLLSDGGNGAYVSGVKAE
                     YAKVREAHLRAEQAKRRITIAAARANRFKIDWPTTKPKRPVFLGRKVFLDYSLSELVP
                     YIDWTPFFQTWELAGRYPRILDDAKVGTEARKLFDDAQLMLKRLVSGRWVRANAVVGF
                     WRANAVGDDIELYDDDKRAKPIAVLHTLRQQIARDPGRDRAHTALADFIAPKETGIAD
                     YVGAFAVTTGIGEEAAIEAHIAKTDDYGRIMLKALADRLAEAFAERMHERV"
     assembly_gap    1332..1371
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1372..2286)
                     /locus_tag="PK264_24480"
     CDS             complement(<1372..2286)
                     /locus_tag="PK264_24480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRD79050.1"
                     /translation="MTTSAASSFINIGERTNVTGSAKFRKLIEANDYAAALAVAREQV
                     ESGAQVIDVNMDEGLLDSERAMVTFLNLIASEPDIARVPVMIDSSKWSVIEAGLKCVQ
                     GKAIVNSISMKEGEGPFLEQARKVLQYGAAVVVMAFDERGQADTADRKVEICKRAYEL
                     LTETVGFPPEDIIFDPNIFAVATGIEEHNGYGIAFIEATRRIKEACPHAHISGGVSNL
                     SFSFRGNEVVRQAMHSVFLYHAIKAGMDMAIVNAGQLPVYDDIDPALRALAEDVILNR
                     RPDATDRLLEAAQRFKGDGAKRNEADLAW"
     gene            complement(2388..>2682)
                     /locus_tag="PK264_24485"
     CDS             complement(2388..>2682)
                     /locus_tag="PK264_24485"
                     /inference="COORDINATES: protein motif:HMM:NF021148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HRD79051.1"
                     /translation="YAVKEHDTTFSGRSLFRTSIDLPANVSVGSFETRVFLFREGKLL
                     SQYLARLNLEREGLERLIHGFAFQYPWFYGITTVLIALASGILASAVFSKARH"
BASE COUNT          443 a          863 c          854 g          482 t
ORIGIN      
        1 cacccgctca tgcatccgct cggcgaacgc ctcggcaagt cgatcggcca gcgccttcag
       61 catgatgcgg ccgtagtcgt cggtcttggc gatgtgggcc tcgatcgcgg cctcctcgcc
      121 gatgccggtg gtcacggcga atgcgccgac gtagtcggcg atcccggtct ccttcggcgc
      181 gatgaagtcg gcaagcgcgg tgtgcgcccg atcgcgtccg ggatcgcgcg cgatctgctg
      241 gcgaagcgta tgcaggaccg cgatcggctt cgcgcgcttg tcgtcgtcgt agagctcgat
      301 gtcgtcgccg acggcattgg cccgccagaa cccgacgacc gcatttgccc gcacccaccg
      361 gcccgaaacg aggcgcttca gcatgagctg cgcatcgtcg aaaagcttgc gggcctcggt
      421 gccgaccttc gcgtcgtcga ggatgcgcgg atagcggccc gcgagctccc aggtctggaa
      481 gaagggcgtc caatcgatat agggcaccag ctcggagagg ctgtagtcga ggaacacctt
      541 gcggccgaga aatacaggcc tcttcggctt cgtcgtcggc cagtcgatct tgaagcggtt
      601 cgcccgagcc gcagcgattg tgatgcgccg cttcgcctgc tcggccctga gatgcgcctc
      661 gcgcaccttg gcgtactcgg ccttgacacc gctcacgtag gcgccattac cgccgtccga
      721 gagcaggctc gagacgacgc cgaccgcccg gctcgcgtcg gtcacataga tcgcctggcc
      781 acgcttgtag ttcgggctga tcttcaccgc cgtgtgcaca cggctcgtcg tcgccccgcc
      841 aatgagtagt gggaccgaga agccctcgcg ctccatctcg gcagccacgt ggcacatctc
      901 gtcgagtgac ggcgtgatga ggcccgacag tccgatgacg tcggcgttct cgcgcttggc
      961 ggtctcgagc accttggccg cggggaccat gacgccaagg tcgatcacct cgtagttgtt
     1021 gcattggagg acgacgccga cgatgttctt gccgatgtcg tgcacgtcgc ctttcaccgt
     1081 cgccatgatg accttgccgg cggccgggcg caggtcgagg ccggtcgcct tcttctcggc
     1141 ctcgaggaac ggctggaggt aggcgacggc ctgcttcatg acgcgggcgc tcttgacgac
     1201 ttgcggcagg aacatcttgc ccgcgccgaa gagatcgccg acgacattca tgccgctcat
     1261 cagtggacct tcgatcacgt cgagggggcg cttcgccgcg gcgcgagcct cttccgtgtc
     1321 agcctcgatg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccaggcgag
     1381 gtcggcttcg ttccgcttgg cgccgtcgcc cttgaagcgc tgagcggctt cgagcaggcg
     1441 atctgtggca tccgggcggc gattgaggat cacgtcctcg gcgagcgcgc gcagcgcggg
     1501 gtcgatgtcg tcgtagaccg gcaactgacc tgcgttgacg atcgccatgt ccatgccggc
     1561 cttgatggcg tggtagagaa acaccgaatg catcgcctgg cggacgactt cgttgccgcg
     1621 gaacgagaac gacaggttcg agacgccgcc cgagatgtgc gcgtgggggc aggcctcctt
     1681 gatgcgccga gtcgcctcga tgaaggcgat gccgtagccg ttgtgctctt cgatgccggt
     1741 cgcgaccgcg aagatgttgg gatcgaagat gatgtcctct ggcggaaagc cgacagtctc
     1801 ggtcagcagc tcataggcgc gcttgcagat ctcgaccttg cggtcggcgg tgtcggcctg
     1861 accccgctcg tcgaaggcca tgacgacgac cgcggcgccg tactgaagga ccttgcgcgc
     1921 ctgctcgagg aacgggccct cgccctcctt catggaaatc gagttgacga tcgccttgcc
     1981 ctgcacgcac ttgagaccgg cctcgatcac cgaccacttg gagctgtcga tcatcaccgg
     2041 cacgcgggcg atatccggct cggaggcgat gaggttcaag aacgtcacca tcgcccgttc
     2101 gctgtcgagc aggccctcat ccatgttgac gtcgatcacc tgcgcgccgc tctcgacctg
     2161 ctcgcgggca accgcgagcg ccgcggcata gtcgttggcc tcgatcagct tcctgaattt
     2221 tgccgaaccg gtgacattgg tccgctcgcc gatattgatg aagctggacg ccgctgaggt
     2281 ggtcatcgct gctgcctgcc aagtgatgaa ctgcagccca gccgctctcg ccgggcgccc
     2341 ccccttatgg agcaaccccg aggcccgctc aacacgcaag acggtcctca gtgccgcgcc
     2401 ttgctgaaca cggcggaagc gaggatgccc gaagcgagtg cgatcagcac ggtcgtgatg
     2461 ccgtagaacc acggatactg gaaggcaaag ccgtggatca gcctctccag accctcccgc
     2521 tcaagattga ggcgcgcgag gtactggctc agcaacttgc cttcacggaa gaggaacaca
     2581 cgggtctcga aagaaccgac tgagacgttt gccggcagat cgatcgaggt cctaaacagg
     2641 ctccggcccg agaacgtcgt gtcgtgctcc ttgacggcat ag
//
LOCUS       DAORNH010000420         2669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_28927_length_2669_cov_1.538638, whole genome shotgun sequence.
ACCESSION   DAORNH010000420 DAORNH010000000
VERSION     DAORNH010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28927_length_2669_cov_1.538638"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PK264_24490"
     CDS             complement(<1..501)
                     /locus_tag="PK264_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRD79052.1"
                     /translation="MRTLLIAEHDNTTLKDATNRTLTAAKAMGGDVHVLVAGKGCRPA
                     AEAAARLDGVAKVLFVDDAAYQHALAEPLAALILSLAEPYEAIIAAATNSGKNFMPRV
                     AALLDVMQISDILKVEGPDTFERTIYAGSAIQTVKSKDKKKVVTVRTAAFPATGEGGI
                     APIEEAK"
     assembly_gap    553..632
                     /estimated_length=80
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<633..>1075)
                     /locus_tag="PK264_24495"
     CDS             complement(<633..>1075)
                     /locus_tag="PK264_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRD79053.1"
                     /translation="VSIGPQQAQETIRTALAMGADHGILVKVDGTVEPLSVAKILKGV
                     VEAVQPDLVILGKQAIDDDCNQTGQMLAALLGWPQGTFASKVAVEDTAFLVTREIDGG
                     LQTVRLNRPAIITTDLRLNEPRHASLPNIMKAKKKPVDEKTPGDY"
     assembly_gap    1076..1101
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1102..1234)
                     /locus_tag="PK264_24500"
                     /pseudo
     CDS             complement(1102..1234)
                     /locus_tag="PK264_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657517.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
     gene            complement(1381..>2669)
                     /locus_tag="PK264_24505"
     CDS             complement(1381..>2669)
                     /locus_tag="PK264_24505"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HRD79054.1"
                     /translation="IEGSPSIDVSKLYASRFGSYANKRFIRAMLSEAHVFGTVDLSGE
                     RLAGRPARTSALYDHFKAKGAHFSAQLGWETPMWFSRGNARSNGVEEARAEALDVRES
                     VGILDLTALAKYEVSGPAASTFLDRLCARRLPAVGSIVSTPMLNERGQVMCFTTIARI
                     EPDRFYLTAAPQAELHHYSWMNEQLPRDGSVQLANVTGRYGALLLSGPRAREVLAKVS
                     EANLSNEVFPWLTVSDIDIAYGRARALRFNMVGELGWELHHPLEYQTGIYEALAAAGE
                     EFQIVDFGFKAYDTLRLEAGWPLWGADFNSSQTATSAGLSRFVDPSKEGFIGRRAILD
                     TAQPVLSRLVHISFDLDGSPPEMGSLLFDGDDVVGHVHSSAYGPSIGSGIAFASVKDG
                     FGRGGKTINCLVRGSRLPVNILERALHDPESLRVSS"
BASE COUNT          534 a          783 c          708 g          538 t
ORIGIN      
        1 cttcgcctct tcgattgggg caatgccgcc ctcgcctgtc gctgggaagg cggcagttcg
       61 cactgtaact accttcttct tgtccttcga cttcactgtc tggatggcgc taccggcgta
      121 gatagtccgc tcaaaggtgt caggcccctc gactttcaga atgtcggaga tctgcatgac
      181 gtcaaggagc gccgcgacgc ggggcatgaa gttcttacct gaattggtcg ccgccgcgat
      241 gatcgcttca tacggttcgg cgagcgacaa gatgagcgcg gcaagcggct cagcgagcgc
      301 atgctggtaa gcggcatcgt cgacaaagag gaccttggcg acgccgtcga gcctggcggc
      361 cgcttcagcc gccggtcggc agcccttgcc cgcaacgagg acatgcacgt cgccgcccat
      421 cgcctttgcg gccgtgagcg tcctattcgt cgcgtctttg agcgtcgtgt tgtcgtgctc
      481 ggcaatcaaa agcgttctca tcagaggact ccggcttcgt tcttgagttt agcgaggaga
      541 tcggcgacgc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgtaatcg cccggcgtct tctcatcgac
      661 cggcttcttc ttcgccttca tgatgttcgg cagcgacgca tgacgtggct cgttgaggcg
      721 cagatcggtt gtgataattg ccggcctgtt gagccttacc gtttgcaagc ccccgtcgat
      781 ctctcgggtg accaggaaag cagtgtcctc gacagcgacc ttcgaggcaa aagtgccctg
      841 tggccagcca agcaatgccg ccagcatctg tccggtctgg ttgcaatcgt cgtcaattgc
      901 ctgcttgccc aggatcacaa ggtccggttg cacggcctcg acaacaccct tgagaatctt
      961 cgccactgaa aggggctcga ccgtgccatc gacctttacc aagatgccgt ggtcggcgcc
     1021 catggcgagc gcagtgcgga tcgtctcctg cgcctgctgc gggccgatag agacgnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn ncttctacag cgatctcgtc gaacgggttc attgccatct
     1141 tcacattcga aagctcaacc cccgaaccgt ccggcttcac ccggaccttc acattgaaat
     1201 ccaccacccg tttcacggct acgagtactt tcatcaaaac acaacctccc gttccaacac
     1261 gaaaactctc cagacggcgc ttacgtcgag gccccaattc ccccggagcg gcgattgatc
     1321 catgcgttga cacgcgtcga cagtacgaag atgctcggac gaagcatcgt catgtcctcc
     1381 tcagctactg acacgcaggc tctctggatc atgcagggca cgctccagaa tgtttacggg
     1441 cagtctactg cctcgcacca agcagttgat ggtcttgcca cccctcccga aaccatcctt
     1501 tacggaagcg aacgcgatgc ccgaacctat ggatggccca taggccgacg agtggacatg
     1561 tccaaccacg tcgtcgccgt cgaacaagag gctgcccatt tcggggggag atccatcgag
     1621 atcgaacgat atgtggacga ggcgagagag cacgggttgt gccgtatcga ggatcgcgcg
     1681 acgaccaata aagccttctt tactgggatc gacgaagcgg ctgagacccg ctgaagttgc
     1741 tgtctgactt gagttgaagt ctgcgcccca gagtggccat ccagcctcga gccgcagtgt
     1801 gtcgtaagcc ttgaatccaa aatcaacgat ttggaattcc tcacccgcgg ccgcaagcgc
     1861 ttcgtagata ccggtctgat attcgagagg gtggtgaagc tcccatccaa gctcgccgac
     1921 catgttgaag cgcaacgcgc gcgcgcgacc gtacgcaata tcgatatctg agaccgtcaa
     1981 ccaagggaaa acttcattgg acaagttcgc ttcgctcact ttagcgagta cttcgcgggc
     2041 tcttggcccc gacaatagca atgctccata gcgaccggtg acattcgcaa gctgtacgct
     2101 tccgtcccgc ggcaattgtt cattcatcca agagtaatgg tgtagctccg cttgcggagc
     2161 agcagtcaaa tagaaacgat caggctcaat gcgcgcgatt gtcgtgaagc acatcacttg
     2221 accgcgctca ttgagcatgg gggtggaaac aatacttccg acggcgggga gacgacgagc
     2281 gcagagccgg tcaagaaagg tactggcggc tggtccagat acttcatact tcgcaagcgc
     2341 cgtgagatcg agaatgccga cgctttcgcg cacatctagt gcttcggcgc gcgcctcctc
     2401 gacgccattt gaacgcgcat tgcctcgcga gaaccacata ggcgtttccc acccgagctg
     2461 ggctgagaaa tgcgcacctt tagccttgaa gtgatcgtaa agcgcgctcg tccgcgccgg
     2521 ccgacccgct aacctttctc ctgagagatc aacagttcca aacacatgcg cttcgcttag
     2581 catggcgcga ataaaccgct tgttggcgta actgccgaag cggctcgcgt acaacttaga
     2641 gacgtcgatg ctcggactgc cttcgatca
//
LOCUS       DAORNH010000421         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_29587_length_2621_cov_2.737334, whole genome shotgun sequence.
ACCESSION   DAORNH010000421 DAORNH010000000
VERSION     DAORNH010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29587_length_2621_cov_2.737334"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..316)
                     /locus_tag="PK264_24510"
     CDS             complement(62..316)
                     /locus_tag="PK264_24510"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD79055.1"
                     /translation="MRLTDYLAQEKLSAAEFAARIGVHRSTVSRWIEPVISGGVPFRP
                     SWEQIAKIRDVTGGAVTANDFVDVMPTPLPSSEETGKAVA"
     gene            505..1104
                     /locus_tag="PK264_24515"
     CDS             505..1104
                     /locus_tag="PK264_24515"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRD79056.1"
                     /translation="MIANRIRELREAKGLSQDELGAKVGTNKFKVSRIETGETRLTLD
                     LATKIAAALDVSVAEVLNVQAIPTARGLAEEAVSYVGGEGDPLARLADIERNRILYEI
                     KSNALDELGIVAGDIVLIDMSADTVAKVKPLALVIAQVYSGTELLAARTVLRQFVPPA
                     LLVANSRSESHEPINMKIIDAHIKGIIVSHHRRLSGGLS"
     gene            complement(1323..1691)
                     /locus_tag="PK264_24520"
     CDS             complement(1323..1691)
                     /locus_tag="PK264_24520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79057.1"
                     /translation="MSAIIWRALVVAAVLILPAMALAQDDDDGQLGPNAYGDAWGKSS
                     DAERAAFAHAVALIMIDRAREIPGASSDLAKVLAALHACLDRNLRSKRPEDQTVVMLL
                     RTRPLKDTVQMCANVALLTK"
     assembly_gap    1735..1824
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1825..2004)
                     /locus_tag="PK264_24525"
     CDS             complement(<1825..2004)
                     /locus_tag="PK264_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79058.1"
                     /translation="MRLATLALALLAVSAADAVARCEGCGCRGGPGYRGPAGQCVGHE
                     KLLSVCGTPPSTRCTF"
     gene            2125..2373
                     /locus_tag="PK264_24530"
     CDS             2125..2373
                     /locus_tag="PK264_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79059.1"
                     /translation="MTLTRLPTNADDLYALMGPEALLAHLRALNTAIARVTDLPAAAS
                     AGIGELEDALLEHLGRADPDALARELGRLEALEKRALY"
     gene            2373..>2621
                     /locus_tag="PK264_24535"
     CDS             2373..>2621
                     /locus_tag="PK264_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79060.1"
                     /translation="MARFITLANGTRIVPGAVQTIEARALVSPWTDASGRLWPAMVFV
                     ETRHQVVRCPCASLGEAQRLRDEIAEAVERHAAQRAAQH"
BASE COUNT          458 a          849 c          792 g          432 t
ORIGIN      
        1 tcgcccggcc ggcgacgctg gacgctggac gcaacgtacg agaacgcgac gcaggcgcac
       61 ttcacgcgac ggctttgccg gtctcctcgg aggaggggag cggcgtcggc atcacatcga
      121 cgaaatcgtt tgccgtcacc gcgccgcccg taacgtccct gatcttggcg atttgctccc
      181 aagacggacg aaacggcacg ccgccggaaa tgacgggctc gatccagcgg ctcactgtcg
      241 atcggtgcac accgatgcgg gccgcgaatt cggcggccga gagcttttcc tgggcgagat
      301 agtcggtgag cctcatgacg tctccacccg catcacatca tcgatcggac gagatgattt
      361 ttcggcggca ctcgatggcc gacacgccgg gcagggcggc gcgacgtaca tgaagtcgca
      421 aagttgcagt atttgcaact ttagtcaaca ggtggttgca aattttgtaa cgcccctagc
      481 gatcaagcct cgtctaatgg cttgatgatc gcgaatcgta tccgagagct gcgcgaggcg
      541 aagggcctca gtcaggacga gttgggcgcc aaagtcggca ccaacaagtt caaggtctcg
      601 cgcatcgaga ctggagagac gcgattgacc ctcgaccttg cgacaaagat cgccgccgcg
      661 ctcgacgtgt cggtggccga agtgctcaac gtccaggcga taccgacagc gcgcggtcta
      721 gccgaggagg ccgtgtccta cgtcggcggc gaaggagacc ctctggcgcg gctggccgac
      781 atcgagcgga atcgcatcct ctacgagatc aagtctaacg ctctggacga gctaggaatt
      841 gtggccggag atatcgtcct catcgacatg agcgcggata ccgttgccaa ggtgaagcct
      901 ctggcgctgg tgattgccca ggtctacagc ggaacggagt tgctcgcggc ccgaacggtc
      961 ctccgccagt tcgtgccgcc ggcgctcttg gttgccaaca gccgatctga gagccacgag
     1021 ccgatcaata tgaagatcat cgacgctcac atcaaaggca tcatcgtctc gcaccatcgc
     1081 cggctttcgg gcgggctttc gtagaagtgg gcgagtaggt cccagtatgg ccacgcgtgg
     1141 gtcagagcca gcaaccatct ggtctggacc aactgtcgtg tagcaaatat tgcaacttta
     1201 tcgttgacat gcagttgcaa tttttgcaac ttggggggcg caggatcgcg agtcgcccga
     1261 tgaaccattt cgccacagtt cgtgcagcag cagggaatgc cgcatcgtgc aatggttctg
     1321 ggtcacttcg tcagcaaggc gacgttcgca cacatctgga cggtgtcctt cagcgggcgc
     1381 gtgcgcaaga gcatcaccac cgtctgatct tcgggcctct tcgagcgcag gttgcgatcg
     1441 aggcaggcgt gaagcgcggc cagcactttg gcgaggtcag acgaagcgcc cgggatctct
     1501 ctcgcgcgat caatcatgat cagcgcaacc gcatgggcaa atgccgcgcg ctctgcgtcc
     1561 gaggacttgc cccaggcgtc gccataggcg ttaggtccga gctggccgtc atcgtcatcc
     1621 tgagccagcg ccatggccgg caggatcagc accgccgcga cgaccagtgc tcgccaaatg
     1681 atagcgctca tttcgccact cttgccccac ccttaccgcc ttgcctcacc ggttnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnngaaggt gcacctcgtc gagggcggcg tgccgcacac
     1861 cgagagcagc ttctcgtggc cgacgcactg gccggctggt ccccggtagc ccgggccgcc
     1921 gcggcagccg cacccctcgc agcgcgcaac cgcgtcggcg gccgagaccg ccaagagagc
     1981 cagggcgagg gtggcgagac gcatgcacga accccgtcgt cagtgccccg agcatagccc
     2041 gatcccgcga ccttgtccct tgttccgtcg ccctgttcgc gttcgcgtcc cagtcgaccg
     2101 cccctccatc ccggatcaat cgacatgacc ctcacccgac tgccaaccaa cgccgacgac
     2161 ctctatgcac tcatggggcc ggaagcgctg cttgcgcacc tgcgcgcctt gaatacggcg
     2221 atcgcccgcg tcaccgatct ccccgccgcg gcgagcgccg gcatcggcga gctcgaggac
     2281 gcgctcctcg agcatctcgg ccgggccgat ccggacgcct tggcccgcga gctcggtcgg
     2341 ctcgaggccc tcgagaagag ggcgctctac tgatggcccg cttcatcacg ctcgcgaacg
     2401 gaacgcgcat cgtgcccggc gccgtgcaga cgatcgaggc gcgcgccctc gtctccccct
     2461 ggaccgacgc ctccggccgg ctctggccgg ccatggtgtt cgtcgagacg cggcaccagg
     2521 tcgtgcgctg cccgtgcgcc tcgctcggcg aggcccagcg cctccgcgac gagatcgccg
     2581 aggcggtcga gcgccatgcc gcgcaacggg ccgcgcagca c
//
LOCUS       DAORNH010000422         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_30760_length_2546_cov_2.718988, whole genome shotgun sequence.
ACCESSION   DAORNH010000422 DAORNH010000000
VERSION     DAORNH010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30760_length_2546_cov_2.718988"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1143)
                     /gene="mdh"
                     /locus_tag="PK264_24540"
     CDS             complement(178..1143)
                     /gene="mdh"
                     /locus_tag="PK264_24540"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRD79061.1"
                     /translation="MARTRIALIGAGMIGGTLAHLVGLKELGDVVLFDIAEGMPQGKS
                     LDIAQSGAVEGFDCNLKGTNSYADIEGANVVIVTAGVPRKPGMSRDDLLGINLKVMEQ
                     VGAGIKKYAPNAFVICITNPLDAMVWALQKASGLPSHMVVGMAGVLDTGRFRTFLAQE
                     MRVSVEDVTAFVLGGHGDDMVPLTRYSAVGGIPLPDLVKMRWLTEERLEQIVDRTRKG
                     GGEIVGLLKTGSAFYAPASSAIQMAESFLRDKKRLLPCAAYLNGEYGVKGMYVGVPVV
                     IGSGGVERIVEIDLNAKERQMFMKSVESVKSLLDASMKIAPQLAS"
     gene            1486..>2546
                     /locus_tag="PK264_24545"
     CDS             1486..>2546
                     /locus_tag="PK264_24545"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HRD79062.1"
                     /translation="MTTIAFRIGIAAALVGAMSGCAVQDLAPALRPLPKETVMLLGRM
                     GMTTEAPIFIRIFKEESELEVWKARSDGHFYHFKTYPICNWSGELGPKVVQGDKQAPE
                     GFYTVAKGQMNPNSGFHLAFNLGYPNIYDRAHKRTGDFLMVHGKCRSAGCYAMTDALI
                     EEIYALAREAFAGGQDGFQVHAFPFRMTPENLARHRANKWYPFWMTLKEGYDYFETTR
                     RTPAIAVCERRYQVNVKPLTPARVDPEGRCPTFERPKMEAFRPLPAPGSKIAEVPPVI
                     VPGPKMRTAATIGAPPSGAATMFGLTKTLLLGITGGLGYEWYVVYLNDGKSVGIWNRA
                     ANEALRDNGANAATVETWQG"
BASE COUNT          451 a          830 c          840 g          425 t
ORIGIN      
        1 cgtggtcgcg gcgctgcccg gcaagggcaa gcgggcgggc gaagagaagc ggtgacgggc
       61 gccgcggcgt tgactccaag agtgagttcg cgccagcccg tattgacagc gccactgcag
      121 cagtcggctg cagtggcgct ggacgtttca ggggccggtc acccgaccga ctgagcctca
      181 ggaggcgagc tgcggggcga tcttcattga ggcatcgagc agcgacttca cgctctcgac
      241 cgacttcatg aacatttgcc gctccttggc gttgaggtcg atctcgacga tgcgctcgac
      301 accgcccgag ccgatcacca ccggcacgcc gacatacatg cccttcacac cgtactcgcc
      361 attgaggtag gcggcgcagg ggagcaggcg cttcttgtcc ctgaggaagc tctcggccat
      421 ctggatcgcc gaggaagcgg gcgcgtagaa ggccgagccc gtcttcagga ggccgacgat
      481 ctcgccgccg cccttgcgcg tacggtcgac gatctgctcg agccgctcct cggtgagcca
      541 gcgcatcttg acgaggtccg ggagcgggat gccgccgacc gcggaatagc gcgtcagcgg
      601 caccatgtca tcgccgtggc cgccgagcac gaaggcggtc acgtcctcga cggagacgcg
      661 catctcctgc gcgaggaagg tgcggaatcg gcccgtatcg agcacgcccg ccatgccgac
      721 gaccatatgg gatggcaggc ccgaggcctt ctggagcgcc cacaccatgg catcaagcgg
      781 attggtgatg cagatcacga acgcgttcgg cgcgtacttc ttgatgccgg caccgacctg
      841 ttccatcacc ttgaggttga taccgaggag gtcgtcgcgg ctcatgccgg gcttcctcgg
      901 cacgccggcg gtgacgatca ccacgttggc gccctcgatg tcggcgtagc tgttggtgcc
      961 cttcaggttg cagtcgaagc cctcgacggc gccggactgg gcgatgtcga gcgacttgcc
     1021 ctgaggcatg ccctcggcga tgtcgaagag gacgacgtcg ccgagctcct tcaagccgac
     1081 gaggtgcgcg agtgtgccgc cgatcatgcc ggcgccgatg agcgcaatcc gtgtgcgcgc
     1141 catgctctga tgtctccgtt tgagcggggc cttgcgcccc gagcctgagc cccgaagtgg
     1201 caatcgaccg gccgagcgcg atggcccgcg ccgggttgcc ggctccgctt agccgcaatt
     1261 gcccacggcg gcaagctcgc cccttggatg agagccgggc catgagagcc ccactttcgc
     1321 caaagccgcg ccatggtttt cggcgacggt cgcgccgggt tgcgaatccg gtgggaacag
     1381 gctagcggta gtccttgggg gttaaccgct tgttaaccaa tcggacttga aggtaggcgc
     1441 cgtgcaccag gagcactgtg tccattcgtg aagaacctgg ggaccatgac aacgatcgca
     1501 ttccgtatcg gcatcgcagc ggcgctcgtc ggcgctatga gcgggtgcgc cgtgcaggac
     1561 ctcgcaccgg cgctgcggcc cctccccaaa gagacagtca tgctgctcgg ccgcatgggc
     1621 atgaccaccg aggcgcccat cttcattcgc atcttcaagg aggaatccga gcttgaggtc
     1681 tggaaggccc gctcggacgg ccacttctac catttcaaga cctacccgat ctgcaactgg
     1741 tcaggcgagc tcggtccgaa ggtcgtgcag ggcgacaagc aggcgcccga aggcttctac
     1801 acggtggcga agggccagat gaacccgaat tcggggttcc acctcgcttt caatctcggc
     1861 taccccaaca tctacgatcg ggcccacaag cgcaccggcg acttcctgat ggtgcacggc
     1921 aagtgccggt cagccggctg ctatgcaatg accgacgcgt tgatcgagga gatctatgcg
     1981 ctcgcacgag aggccttcgc gggcggccag gatggcttcc aggtgcacgc gttcccgttc
     2041 cgcatgacgc ccgagaacct cgcccgccac agagccaaca agtggtatcc cttctggatg
     2101 accttgaagg agggctacga ctacttcgag acgacgcgcc ggacgccagc gattgcggtt
     2161 tgcgagcggc gctatcaggt gaacgtgaag ccgctgacgc cggcgcgggt cgatccggag
     2221 ggccgctgcc cgacgttcga gcgaccgaag atggaagctt tccgaccgct gcccgccccg
     2281 ggttcgaaga tcgccgaggt tccacctgtc attgttccgg gaccgaagat gcggacagcc
     2341 gcgacgatcg gcgcgccgcc atcgggcgct gccacaatgt tcggcctcac caagacgcta
     2401 ctgctcggca tcacgggcgg cctcggctac gagtggtatg tcgtctatct caatgacggc
     2461 aagagcgtcg gcatctggaa ccgcgccgcc aacgaggccc tgcgcgacaa cggcgcgaac
     2521 gccgccacgg tcgagacttg gcaggg
//
LOCUS       DAORNH010000423         2530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_31017_length_2530_cov_2.789495, whole genome shotgun sequence.
ACCESSION   DAORNH010000423 DAORNH010000000
VERSION     DAORNH010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31017_length_2530_cov_2.789495"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK264_24550"
     CDS             complement(<1..351)
                     /locus_tag="PK264_24550"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HRD79063.1"
                     /translation="MNIVSLIMNMITPDMIMKIASALGLDKMLIQKAIGAAVPSILSG
                     LAGAAAKPDGARGLADMFAKQDPGLLGSLAGMIGGAGQSKLVDSGSSMLSGLLGGAAT
                     SALTGAIGKFAGIGD"
     gene            complement(592..1131)
                     /gene="ruvC"
                     /locus_tag="PK264_24555"
     CDS             complement(592..1131)
                     /gene="ruvC"
                     /locus_tag="PK264_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRD79064.1"
                     /translation="MDKRAPIRIIGIDPGLRRTGWGVIEADGARLAYIASGHITSESD
                     DPLAYRLLALYEGLSRVLAALKPREAAVEETFVNDNARATLKLGQARGIALLAPATAG
                     LPVAEYPPNLIKKTVVGAGHAEKHQIQAMIGFLLPKAQFESADEADALAIAICHASHR
                     SSRALAQPRTVPARLSVER"
     gene            complement(1191..2093)
                     /gene="hemF"
                     /locus_tag="PK264_24560"
     CDS             complement(1191..2093)
                     /gene="hemF"
                     /locus_tag="PK264_24560"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HRD79065.1"
                     /translation="MDLIELDAAKAAARHWFEELRDDICAAFETLERELPQGAPLSER
                     PDGRFKRTPWTRSDHTGAPGGGGVMSMMSGRVFEKVGVHTSTVHGEFAPEFRKQIPGA
                     EEDPRFWASGISLIAHPQNPNVPAVHMNTRFVVTTKWWLGGGADLTPVLARRRTQEDP
                     DTIAFHAAMAAACAKHGLANYARYKTWCDEYFYLPHRKEMRGIGGIFYDYLTPAPETG
                     GFEAGLAFTKDVGRAFRDIYPRLVRANFAKPWGEADREEQLVRRGRYVEFNLLYDRGT
                     VFGLKTGGNVDSILSSMPPIVKWP"
     gene            complement(2133..>2530)
                     /locus_tag="PK264_24565"
     CDS             complement(2133..>2530)
                     /locus_tag="PK264_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HRD79066.1"
                     /translation="GLAPPPGSRSRADYYRWLFFAAGPLEAAVADKVLGAAVPAEKQG
                     MIGYGNFDLVVATLDQALAGKTALVDGRFSAADVYVGSHLMWGKQFGTLPKRPSFESY
                     CAGLAGRPASTRASAIDDALVPNHPMPSR"
BASE COUNT          406 a          815 c          886 g          423 t
ORIGIN      
        1 gtcgccgatg cccgcgaact tgccgatggc gcctgtgagc gcgctcgtcg ccgcgccgcc
       61 gagcaggcca gagagcatcg acgagccgct gtcgacgagc ttcgactggc cggcgccgcc
      121 gatcatgccg gcgaggctgc cgaggagacc cgggtcctgt ttcgcgaaca tgtcggcgag
      181 gccgcgtgcg ccatcgggct tggcggcggc cccggcgaga cccgagagga tcgacggcac
      241 ggcagcgccg atggccttct gaatgagcat cttgtcgagg ccgagtgccg acgcgatctt
      301 catgatcatg tcgggggtga tcatgttcat gatcagggaa acgatattca tccacttacc
      361 tccacgggcc tcacgccact cgtgcgtcga ggacggttgc tggctctgtc gcgacagcct
      421 gagtcggagg acccgcgcgg cgcgcaaatg ccatcgccat gtcgggcggc acgtttcgtc
      481 gccacccgca gtcctttggc gcatgtccat tagttgtgct gacgcaccat cgtcaagcgc
      541 ggggaaatgt tgccctagga caacatttat tctcattccg tgcaaaatcg atcagcgttc
      601 gacagatagg cgcgccggga cggttcgggg ctgcgcgagc gcccggctcg agcgatggct
      661 cgcatgacaa atggcaatgg ccagcgcgtc ggcctcgtcc gcgctctcga actgggcctt
      721 gggcaggagg aagccgatca tggcctgaat ctggtgcttc tcggcgtggc ccgctccgac
      781 gacggttttc ttgatgaggt tcggcggata ctcggcgacc ggaaggccgg ccgtggcggg
      841 agcgagcagc gcgataccgc gcgcctggcc gagcttcagc gtcgcgcgcg cattgtcgtt
      901 gacgaaggtc tcctcgacgg cagcctcacg cggcttcaaa gcggcgagca cgcgcgagag
      961 gccctcgtag agtgccagca accggtaggc gagcggatca tcgctctcgg acgtgatgtg
     1021 gcccgaggca atgtaggcga ggcgcgcgcc gtcggcctcg atcacgcccc agccggtgcg
     1081 gcggaggcca gggtcgatgc cgatgatgcg aatcggtgcg cgcttgtcca tgccgcgcgg
     1141 ctagcacgac gcccgaccgg cgtcgatgcg ccaccagcac gccccacgcc tcacggccac
     1201 ttcacgatag gcggcatgct cgagaggatg ctgtcgacat tgccgccggt cttcagaccg
     1261 aacacggtgc cgcggtcgta gagcaggttg aactcgacat agcggccgcg gcggacgagc
     1321 tgctcctcgc gatcggcctc gccccacggc ttggcgaaat tggcccggac gagcctcgga
     1381 tagatgtcgc gaaaggcgcg gcctacatcc ttcgtgaacg cgagtccagc ttcgaagccg
     1441 cccgtttccg gtgcgggtgt caggtagtcg tagaagatgc cgccgatgcc gcgcatctcc
     1501 ttgcggtgag ggaggtagaa gtactcgtcg caccacgtct tgtatcgcgc gtagttggcg
     1561 agcccgtgct ttgcgcaagc tgccgccatc gccgcgtgga aggcgatcgt gtcggggtcc
     1621 tcctgcgtgc gccgccgggc gagcacgggt gtgaggtcgg cgccaccacc gagccaccac
     1681 ttggtggtga cgacgaagcg ggtgttcata tgcacggcgg gtacgttcgg gttctgcgga
     1741 tgcgcgatga gagatatccc cgaggcccag aaacgcggat cctcctcggc gccggggatc
     1801 tgcttcctga actcgggcgc gaactcgccg tgcaccgtcg aggtgtgcac accaaccttt
     1861 tcgaagactc ggccgctcat catgctcatg acgccgccgc cgcctggcgc gccggtgtgg
     1921 tcagaccgcg tccagggcgt gcgcttgaac cggccgtcgg ggcgctccga gagcggcgcg
     1981 ccttgcggca gctcgcgctc gagcgtctcg aaggcggcac agatgtcgtc gcgcagttcc
     2041 tcgaaccagt gtcgggcggc cgctttggcg gcgtcgagtt cgatcagatc catgggtcac
     2101 cgagtgcttt cgtatggccg gcgggtgccg cctcagcgcg agggcatcgg atggttcggc
     2161 accagggcat catcgatcgc cgaagcgcgg gtcgacgctg ggcgaccggc gagcccggca
     2221 caataggact cgaatgacgg acgcttcggc agggtgccga actgcttgcc ccacatgagg
     2281 tgcgagccga cgtagacgtc cgccgccgag aagcggccgt cgacgagtgc cgttttgccg
     2341 gcgagcgcct ggtcgagcgt ggcgacgacg aggtcaaagt tgccatagcc gatcatgccc
     2401 tgcttttccg cggggacagc ggcgccgagc accttgtccg cgaccgcagc ttcgagcggc
     2461 ccggccgcga agaacagcca gcgatagtag tcggcgcgcg accggctgcc tggcggcggg
     2521 gcgaggcccg
//
LOCUS       DAORNH010000424         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_31384_length_2510_cov_2.308758, whole genome shotgun sequence.
ACCESSION   DAORNH010000424 DAORNH010000000
VERSION     DAORNH010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31384_length_2510_cov_2.308758"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..926)
                     /locus_tag="PK264_24570"
     CDS             complement(177..926)
                     /locus_tag="PK264_24570"
                     /inference="COORDINATES: protein motif:HMM:NF041238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase-related (seleno)protein"
                     /protein_id="HRD79067.1"
                     /translation="MEGGPTIEIGGERLPIRPNLATAIEAAWGRLAGAGTWWTGSERL
                     AIAAEVRRARSCELCERRKSAVSPSAIAGRHVATGLLSVDAVEAAHRLATDASRLTAA
                     TIQALTDGSLGEEHYVELVAVVAMITALDSFDAGLGRPLRELPEPVGGRPSRLRPAQA
                     VRSLAWVATVQPGAYTESEFDPYKAHGDKNIHQALSLVPAEVASFFDLDVELYLRDHE
                     IRDFSREFRAITHRQIELIAGRTSALNRCFY"
     gene            1125..>2230
                     /locus_tag="PK264_24575"
     CDS             1125..>2230
                     /locus_tag="PK264_24575"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HRD79068.1"
                     /translation="MRRLLASLLAMLATLLAAPIASAGPNDIAIVIGNKGYRGGTPEV
                     KFAVNDMEGFAKAATDVFGVPRDQVRKFPDASLGTFFELFGRPGVPGEPGAGSLLERL
                     IQSRSTRIFIFYSGHGVPLISGPKPRSHLLPIDGSPAAVANTAYALDDLIAAVKSVLA
                     RKAPDGQAIIILDACFSGSSGDGGSLVPATSGTAFNIDMAAIASSERITVLAASSDLE
                     LAHWDTERRHGLFTDTLLDGLYGEADEKRSGGNGDRAISLQELKGFVAARMRARLLSL
                     HRDRPPTQSPHFTGRATAEIVKLAASLPERNPGAREADERECQFLLRFGQLAAIESFM
                     GRSTCVNCPCRTALEVKRRSLSAQSSTCETDERL"
     assembly_gap    2231..2298
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2299..>2510
                     /locus_tag="PK264_24580"
     CDS             <2299..>2510
                     /locus_tag="PK264_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79069.1"
                     /translation="PAVRAAAEARVKQAEEEAARAKKRIDDERNRLDEEQRRVEAERK
                     RIAALEDEARRRAEDEARRRAEDDAR"
BASE COUNT          417 a          851 c          793 g          381 t
ORIGIN      
        1 cgcgcctcgc cctgccggcg cggatcatcg ccgaccagcg cctcgacgag ctcgatcagc
       61 gcgcggccct cgggcacccc gctcgccgcg ccgtcgccgc cgacgatggc gccgagatcg
      121 aagctctctc cacttgcctt gctgctctca cggagcagtg ccgcatggct ggtcgttcaa
      181 taaaaacatc ggttgagcgc cgaagtccgc ccagcgatga gctcgatctg gcggtgcgtg
      241 attgcccgga actcgcggga aaagtcgcgt atctcgtgat cacggagata aagctcgacg
      301 tcgagatcaa agaagctcgc gacctcggcc ggaacgaggc tcagcgcctg atggatgttc
      361 ttgtctccgt gcgccttgta ggggtcgaac tcactttcgg tgtaggcgcc gggctgcaca
      421 gtggcgaccc aggcgagtga gcgcacggct tgtgccggac gaaggcgcga cgggcgtccg
      481 ccgaccggct cgggaagctc tcggagcggc cgaccgagtc cggcgtcgaa ggaatcgagg
      541 gccgtgatca tcgcaaccac ggcgacgagc tctacatagt gctcctcgcc aaggctcccg
      601 tccgtgagtg cctgtatggt cgccgccgtg agccgcgaag cgtcagtcgc caaccgatga
      661 gcagcctcga cggcatccac agaaagcaga cctgtggcca catgccgacc agcgatcgca
      721 ctcggcgaaa cggcggactt ccgcctctcg caaagctcgc agctgcgcgc gcggcgtacc
      781 tccgccgcaa tggcgagccg ttccgagcct gtccaccacg tgccggcacc ggcaagccgc
      841 ccccaagccg cctcgatagc agtcgcaagg ttggggcgaa tgggcagccg ttcgccgccg
      901 atctcgatgg ttgggccacc ttccatggag tgagattaac cgagcctccc gaacccgcat
      961 agccagaaaa tggcggcgag cgacgctccg gttgcagttc tgtatccgcc gctcgccacg
     1021 aaatctgcgc cagggggaca ttttcgaagt catggccctt gaaagcattt ttcgcgcgct
     1081 ctagcttcga gcattcgccc gcaatcggtt gccggaggcc tgccatgcgc cgcctgctcg
     1141 cgtcactgct tgccatgctt gcgacacttc ttgccgcgcc gatcgcctcg gccgggccca
     1201 atgacattgc catcgtcatc ggcaacaagg gctaccgggg cggcacaccc gaggtgaaat
     1261 tcgccgtcaa cgatatggag ggcttcgcca aggcggcgac cgacgtgttc ggcgtgccgc
     1321 gggaccaggt gcgcaagttt ccggatgcga gtctcggcac attcttcgag ctgttcgggc
     1381 gccctggtgt gccgggcgag cccggcgccg gtagcctgct cgagcggctg atccagagcc
     1441 ggtcgacacg catcttcatt ttctactccg gccatggcgt gccgctgatc tcgggcccga
     1501 agcctcgctc ccacctcctc cctatcgacg gcagccctgc cgcggtggcg aataccgcct
     1561 acgcgctcga cgatctcatc gccgcggtca agagcgtgct ggcgaggaag gccccggacg
     1621 gacaggcaat catcatcctc gacgcctgct tctcgggctc gtccggtgac ggcggctcac
     1681 tcgtccctgc gacctccggc accgcgttca acatcgacat ggccgccatc gccagctcag
     1741 agcgtatcac cgtactcgca gcgtcgtcgg acctggagct cgcacattgg gacaccgagc
     1801 gccggcacgg cctcttcacc gacactctcc tcgatggcct ctatggcgaa gcagacgaga
     1861 agcggtcagg tggcaacggg gaccgcgcca tctcactcca ggagctgaag gggttcgtcg
     1921 cagcgcggat gcgggctcgc ctcctctcac tgcatcgcga ccggccgccg acccaatccc
     1981 cgcacttcac cggccgggcg acggccgaga tcgtgaaact ggcggcgtcg ctgccggagc
     2041 gcaatccggg agcccgcgaa gccgacgagc gcgaatgcca attccttctg cgcttcggcc
     2101 agctggcggc gatcgaatcc ttcatgggcc gctcgacctg cgtgaactgc ccgtgccgga
     2161 cggcgctcga ggtgaaacga cgctcgctca gcgcccagtc gtcgacctgc gagacggacg
     2221 agcggctctg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnngc ccggcggtgc gggctgcggc cgaggcccgc gtcaagcagg
     2341 ccgaagagga ggcggcgcgc gcgaagaagc gcatcgacga tgagcgcaat cgactcgacg
     2401 aggagcagcg ccgggtcgag gcggagcgca agcgcatcgc cgccctcgag gacgaagcgc
     2461 gacgccgcgc cgaggacgag gcccgccgcc gggctgagga cgacgcccgg
//
LOCUS       DAORNH010000425         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_32810_length_2423_cov_1.933699, whole genome shotgun sequence.
ACCESSION   DAORNH010000425 DAORNH010000000
VERSION     DAORNH010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32810_length_2423_cov_1.933699"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK264_24585"
     CDS             complement(<1..438)
                     /locus_tag="PK264_24585"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRD79070.1"
                     /translation="MSVVLAIDLGTSRLKAGLVDVTGALVAEAAVAAPPADRAAGAHE
                     IDPEDWWRGFAALAGELLERAPADFGSIVAVAVCGATRTQVLIDAQGGHVGPAIGWAD
                     ALAGEDAASLLARSAPGSDELRHVNAFHPLARLAWLSRTAPERL"
     gene            complement(435..884)
                     /locus_tag="PK264_24590"
     CDS             complement(435..884)
                     /locus_tag="PK264_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HRD79071.1"
                     /translation="MAPHGMLRRVAKVVGALVAATVMAWPALADLKLCNASSSRVGVA
                     IGYQDSKGWATEGWWNIAPQTCETLLKGTLSSRYYYIHAIDYDRGGEWGGGSFMCTAE
                     KVFLIRGVQDCSKRGMKRTGFFEVDTGEAKDWTVRLTDPAASAAKAK"
     gene            complement(899..>1200)
                     /locus_tag="PK264_24595"
     CDS             complement(899..>1200)
                     /locus_tag="PK264_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HRD79072.1"
                     /translation="EYALALALALDGERDISAIAADTDGIDGGEGSAADPAGAIVCPA
                     TVVRGAAEGLSAARHLANNDATPYFAALGDLVVTGTTHTNVNDFRAIVIDRGVDA"
     assembly_gap    1201..1210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1211..>2083)
                     /locus_tag="PK264_24600"
     CDS             complement(<1211..>2083)
                     /locus_tag="PK264_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRD79073.1"
                     /translation="AAATEAHYRRAGHLDRIAGFTTAAHGYRARLPGSVPSHIEIIEA
                     RHPTPDAASVAAAERAIRQASAATADDIVLVLLSGGASALWTAPAEGLDLAAKTTLTR
                     GLLKCGADIHEMNTVRRHLSRIKGGRLAAAANGAEVVTLAISDVPGDDPATIGSGPTV
                     PDPTTLAEARAVLERRRPAFDRLGLEIPESIVRALADRRNETMKSGHPAFARTRYEII
                     ATPRRSLDAAALAARQEGYEVVDLGDTVTGEARDIASAHARMAVEIAAAGRRAVILSG
                     GELSVTVANKAGRGG"
     assembly_gap    2084..2156
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2157..2236)
                     /locus_tag="PK264_24605"
     CDS             complement(<2157..2236)
                     /locus_tag="PK264_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79074.1"
                     /translation="MAERHRRLLRELYDTAVAAAHPSTCLA"
BASE COUNT          346 a          817 c          807 g          370 t
ORIGIN      
        1 gagccgctcc ggcgcggtcc gggagagcca ggcgagccgc gccagcgggt ggaacgcgtt
       61 cacgtggcgc agctcatcgc tgccgggcgc gctgcgggcg agcaaggagg ccgcgtcctc
      121 gccggcaagc gcatctgccc aaccgatggc cggtccgacg tgtccgcctt gcgcatcgat
      181 cagcacttgc gtgcgcgtcg caccgcagac ggcaacggcg acaatcgagc cgaagtccgc
      241 cggtgcacgc tcgagaagct cgccggcgag ggccgcgaac ccgcgccacc aatcctccgg
      301 gtcgatctcg tgcgcacccg cggctcggtc cgctggtggc gccgcgacgg cggcttccgc
      361 aacgagcgca cccgtcacat cgacgagccc cgccttcagc cgcgacgtgc cgagatcgat
      421 cgcgaggacg acgctcactt cgctttggcg gccgacgccg cgggatcggt gagcctgacc
      481 gtccagtcct tcgcctcgcc cgtgtcgacc tcgaagaacc ctgtccgctt cataccccgc
      541 ttgctgcaat cctggacgcc gcggatgagg aaaaccttct ctgccgtgca catgaacgag
      601 ccaccgcccc actcaccacc gcgatcgtag tcgatcgcgt ggatgtagta gtagcggctc
      661 gacagcgtgc ccttgagcag cgtctcgcag gtctggggag cgatgttcca ccagccctcg
      721 gtcgcccagc ccttggaatc ctggtagccg atcgccacgc cgaccctgct cgacgaggca
      781 ttgcagagtt tgagatcggc cagcgctggc caggccatca cggttgccgc cacaagagcg
      841 ccgaccacct tcgccacgcg ccgcaacatg ccgtggggtg ccattctttc gtcccttctc
      901 acgcgtcaac cccgcgatcg atgacgatcg cgcggaagtc gttgacattg gtatgtgtcg
      961 tcccggtgac cacgagatcg ccgagcgccg cgaagtaggg cgtcgcgtcg ttgttggcga
     1021 gatggcgcgc cgcggaaagg ccctcggcgg caccacgtac caccgttgcc gggcagacga
     1081 tggcgcctgc ggggtcagcg gcactgccct ccccgccgtc gatgccgtcg gtatcggcgg
     1141 caatggccga gatgtcgcgc tccccgtcga gcgcgagtgc cagggcgagg gcatattcct
     1201 nnnnnnnnnn cccgccgcgc ccggccttgt tggcgacggt gacggaaagc tcgcccccgg
     1261 agaggatcac cgcacgccgc cccgccgccg cgatctcgac ggccatgcgc gcgtgtgcgg
     1321 acgcgatgtc gcgcgcctcg cccgtgaccg tatcaccgag atcgacgacc tcgtaaccct
     1381 cctgtcgcgc agcgagggca gccgcgtcga gcgatcggcg cggcgtcgcg atgatctcgt
     1441 agcgtgtccg tgcaaaagcc ggatggccag acttcatggt ttcgttgcgc cgatcggcaa
     1501 gcgctcgcac gatgctctcc gggatttcga gcccgagccg gtcgaacgcc gggcgccgcc
     1561 gctccaggac ggcgcgggcc tccgcgagcg tcgtcggatc gggcaccgtc ggccccgagc
     1621 cgatggtcgc agggtcgtcg ccgggaacgt cggagatggc gagtgtgacg acttccgcgc
     1681 cattggccgc cgcggcgagc cgtccgccct tgatccgtga gagatgccgc cgcaccgtgt
     1741 tcatttcgtg gatgtcggcg ccgcatttga ggaggccgcg cgtcagcgtc gtcttcgcgg
     1801 cgagatcgag cccttcggcg ggcgccgtcc agagcgccga cgcgccgccc gagagcagca
     1861 cgaggacgat atcgtcggcg gtcgctgccg acgcctgtcg gatggcgcgc tctgctgcgg
     1921 cgacgcttgc tgcgtcgggt gtcggatgcc gcgcctcgat gatctcgata tggctcggca
     1981 cggagcccgg aagcctcgcg cgatagccgt gggcggccgt ggtgaagcct gcgatgcggt
     2041 cgaggtgacc ggcgcggcga tagtgcgcct cggtggccgc agcnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcta
     2161 gacatgtcga gggatgcgcc gccgcgacgg ccgtgtcgta gagttcgcgc aagagacggc
     2221 gatgtcgttc agccatgatc gtgtccgccc gtgccgccgt tgagctgccc tcatgtggag
     2281 gagcgccgga agggccgtca aggtgcttcg cgcgcgaagt gacagcgagc tagcgtgcgc
     2341 gtgttgatag caggctcgga ccgcctgacc tgccgttcat gacatagtgc agtacgatct
     2401 cagccgcagc gccgctcggc gtc
//
LOCUS       DAORNH010000426         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_33042_length_2410_cov_3.087898, whole genome shotgun sequence.
ACCESSION   DAORNH010000426 DAORNH010000000
VERSION     DAORNH010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33042_length_2410_cov_3.087898"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK264_24610"
     CDS             complement(<1..196)
                     /locus_tag="PK264_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79075.1"
                     /translation="MSDEILVGTKKGLFSIVRRGGAWQIGASAFLGDNVSMVLADTRD
                     GARYVALEHGHFGCKMHRAAG"
     gene            complement(243..512)
                     /locus_tag="PK264_24615"
     CDS             complement(243..512)
                     /locus_tag="PK264_24615"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HRD79076.1"
                     /translation="MPRVSFTANIQRHVACESVEAAGGTVREVLDGVFERNPRARSYV
                     LDDQAALRRHMAVFVDGVAIRDRLHLSDRVRADSHIHVMQALSGG"
     gene            complement(520..1836)
                     /gene="trpB"
                     /locus_tag="PK264_24620"
     CDS             complement(520..1836)
                     /gene="trpB"
                     /locus_tag="PK264_24620"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRD79077.1"
                     /translation="MPAAKSPSKSKSAAAKAPRKAAAKQAPKSGARRAADRANSLRNG
                     PDDRGFFGLFGGRYVAETLMPLILDLEKAYGDAKADSAFKASLDHLHKHYSGRPSPLY
                     FAERLTEHLGGAKIYFKRDELNHTGSHKINNCLGQILLARRMGKTRIIAETGAGQHGV
                     AVATVCAKFKLPCEIYMGATDIERQAPNVARMKLLGAKLNSVSSGAGTLKDAMNEALR
                     DWVTNVANTYYIIGTAAGPHPYPAMVRDFQSIIGDEVRAQMLEAEGRLPDTLVACIGG
                     GSNAIGLFHPFLDDKDVAIYGVEAGGHGLEVPNGHAASMTGGRPGVLHGNRTYLLQDD
                     DGQILEGHSISAGLDYPGVGPEHSWLKDTGRVTYVPATDKEALEALQLCTRLEGIIPA
                     LEPAHALAYVTKLAPKLAKDNLLVMNLCGRGDKDLFTVAKALGLKI"
     gene            complement(2168..>2410)
                     /locus_tag="PK264_24625"
     CDS             complement(2168..>2410)
                     /locus_tag="PK264_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRD79078.1"
                     /translation="GGVYGSMMSTPILNAPQSGILGMHRIEERPVVRAGQIVARPMMY
                     LALSYDHRIVDGKEAVTFLVRVKECLEDPQRFVLEL"
BASE COUNT          408 a          818 c          758 g          426 t
ORIGIN      
        1 cacctgcagc acgatgcatc ttgcagccga agtggccgtg ctcgagcgcc acatagcgcg
       61 cgccgtcgcg cgtgtccgcc agcaccatcg agacgttgtc accgaggaac gcgctcgccc
      121 cgatctgcca cgcgccgccg cgccgtacga tcgaaaacag ccccttcttc gtgccaacca
      181 ggatctcgtc gctcatcaca gcctcctcga ggttgccgcc aagccgtaca cggtcgcaga
      241 cctcaaccgc ctgacagagc ctgcatcacg tgaatgtggc tatccgcccg tacgcgatca
      301 ctcagatgca gccgatcgcg gatcgcgacg ccatcaacga acacggccat atgccggcgt
      361 agcgccgcct gatcgtcgag cacgtagctc cgcgcgcgcg gattgcgctc aaacacgccg
      421 tcgagcacct cgcgcaccgt cccgcccgcc gcctcgaccg actcgcaggc gacgtgtcgc
      481 tggatatttg ccgtgaacga aacgcggggc atctccgcct caaatcttca acccgagtgc
      541 cttggcgaca gtgaagaggt ccttgtcacc gcggccgcac aaattcatca cgaggaggtt
      601 gtccttggcg agcttgggcg cgagctttgt cacgtaggcg agcgcatgcg cgggctcgag
      661 cgccgggatg atgccctcga gccgtgtgca cagctgcaat gcctcgagcg cctccttatc
      721 ggtcgccggc acataggtaa cgcggcccgt gtccttgagc caggagtgct ccggaccgac
      781 gccggggtag tcgaggcccg ccgagatcga gtgcccttcg aggatctgcc cgtcgtcatc
      841 ctggaggagg tacgtgcggt tgccgtgcag cacgccgggg cggcctccgg tcatggacgc
      901 cgcatgcccg ttcggcactt caaggccgtg cccgccggcc tcgacgccgt agatggcgac
      961 gtccttgtcg tcgaggaacg gatggaacag accgatcgca ttcgagccgc cgccgatgca
     1021 ggcaacaagc gtatccggca gccggccctc ggcctcgagc atctgcgcgc gcacttcatc
     1081 gccaatgatc gactggaagt cgcgcaccat cgcgggatag ggatgcgggc cggccgccgt
     1141 gccgatgatg tagtaggtgt tcgcgacgtt cgtcacccag tcgcgaagcg cttcattcat
     1201 ggcgtccttg agcgtgcccg cgccgctcga cacggagttg agcttggcgc cgagcagctt
     1261 catgcgagcg acattcggtg cctgccgctc gatatcggtc gcgcccatgt agatctcgca
     1321 cggcagcttg aacttggcgc agactgttgc gacagcgacg ccgtgctgcc cggctcccgt
     1381 ctcggcgatg atgcgggtct tccccatgcg gcgagcgagc aggatttgcc cgaggcagtt
     1441 gttgatcttg tgcgagcccg tgtgattgag ctcgtcgcgc ttgaagtaga ttttcgcgcc
     1501 gcccagatgc tcagtcagcc gctcggcgaa gtagagaggg ctcggccgtc ccgaatagtg
     1561 tttgtggagg tgatcgagcg aggccttgaa agcggagtcc gccttggcgt caccgtaggc
     1621 cttctcgaga tcgaggatca gcggcataag cgtctcggcg acgtaacgtc cgccgaacag
     1681 gccgaaaaaa cctctgtcgt ccggcccgtt gcgcagactg ttggcgcgat cggcagccct
     1741 gcgcgctccg gacttcggcg cttgtttcgc cgcagccttg cgcggggcct tcgctgcggc
     1801 actcttcgat ttgctcggcg acttggcagc gggcatgggc gcatttcctc gagggccgga
     1861 cgcgcactca ttggcaagcg ccgcgccgga aatatctctg gtatcaagta gcgccgatcc
     1921 acgaccgagt tcaagcgcga ggtctcacaa ccgcacgctc gctgcgcgat cggggctctc
     1981 gccccgcgct ccagtgggag ggacaccctc caaaccaccc gctttctcga cttggtctca
     2041 aattaggcag gtttcggggc gccccgcgcg cgtctgcgcg caagctcgaa atcgcccccg
     2101 agtgggtcga gggcggcagc cccgctcgcg aagctaagcc cttactgcaa ctgccgcacc
     2161 atcaccctca aagctcgagc acgaaacgct gcgggtcctc gaggcactcc ttgacgcgca
     2221 cgaggaaggt cacggcctcc ttgccgtcga cgatgcggtg atcgtagctg agggccaggt
     2281 acatcatcgg ccgggcgacg atctgtccgg ctcgcaccac gggccgctcc tcgatcctgt
     2341 gcatgccgag aatacccgac tgcggcgcgt tcaggatcgg cgtcgacatc atggagccgt
     2401 agacgccgcc
//
LOCUS       DAORNH010000427         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_34019_length_2356_cov_2.667536, whole genome shotgun sequence.
ACCESSION   DAORNH010000427 DAORNH010000000
VERSION     DAORNH010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34019_length_2356_cov_2.667536"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PK264_24630"
     CDS             complement(<1..138)
                     /locus_tag="PK264_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRD79079.1"
                     /translation="MKRYRIAAIPGDGIGTEVISAGIEVLEALARRDGSFAITFDHFD
                     WG"
     gene            complement(238..687)
                     /locus_tag="PK264_24635"
     CDS             complement(238..687)
                     /locus_tag="PK264_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79080.1"
                     /translation="MNDPPTSRPRLRFDDVFDIVAIDDSFVDLRVLESLVLRAFRPRA
                     RMRAFLDLASARPSLAALPPHLVIIDDQMGTLRAEDTLAELRGSGYLGPVAVISGGGL
                     PGRGAELLQKGVLAYLAKDDLNITSVITLIDQAMKHLSLWSANRDDR"
     gene            952..1485
                     /locus_tag="PK264_24640"
     CDS             952..1485
                     /locus_tag="PK264_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRD79081.1"
                     /translation="MEKLTPEQKKAADEIVSGPRGRVVGPFNVLLRSPDFMSRLQKTG
                     EYLRFKSAHEPRLSELAILITARHWSQNFEWHHHRPIAEKAGLKADIIDAIAEGRRPA
                     TMAKDEAVIYDLLTELNANKTVSSPTYARALGQFGERGIVDLVGIAGYYSLLAMLMNT
                     AATPLPEGTPPPLPTLP"
     gene            complement(1501..1776)
                     /locus_tag="PK264_24645"
     CDS             complement(1501..1776)
                     /locus_tag="PK264_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034806546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HRD79082.1"
                     /translation="MSMDERIRERLMMALEPRRLDVVNESHMHAGHASSPGTGESHYR
                     VLVVADRFAGQTRVARHRMVNEALKAELAEGVHALAIGAYAPGEPVR"
     gene            1951..>2356
                     /locus_tag="PK264_24650"
     CDS             1951..>2356
                     /locus_tag="PK264_24650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRD79083.1"
                     /translation="MKLDSKYFDSLRSTAEPRSSRREAAHACQWAGCNEPASHRAPMG
                     RGREGRFYRFCLEHARQFNANYNYFQGMSDTEVEGYLKDSVTGHRPTWRMGNDPALGR
                     GARSGASPRSRDGRLDGDDAHEVFEDAYRGRPG"
BASE COUNT          424 a          774 c          748 g          410 t
ORIGIN      
        1 gccccagtcg aaatggtcga acgtgatcgc gaagctgccg tcgcgccggg caagtgcctc
       61 gagcacctcg atgccggccg agatgacctc ggtgccgatg ccgtcaccgg gaatggctgc
      121 aatgcgatag cgcttcatga ggcggggtcc gatcgtggtc gagtgggcgt acgcagacgc
      181 ccttgtcgct cacattcggt cgcgtggcca gcccggtccg ctcggcggtc tgcagcacta
      241 cctgtcgtcg cgattggcgc tccacaggct caggtgcttc atggcctggt cgataagggt
      301 aatcacactc gtgatgttga gatcgtcctt ggcgaggtag gcgagtacgc ccttctgcag
      361 gagctcggcg ccgcggccgg gcaagccacc ccccgaaatg acggcgacag ggcccagata
      421 gcccgagcct cggagctcgg cgagcgtgtc ctcggctctg agcgtcccca tctgatcgtc
      481 gatgatcacg aggtgcgggg gcaatgctgc gaggctcggc cgcgccgatg cgaggtcgag
      541 gaaggcgcgc atgcgcgctc gcggacggaa agccctgagc acaaggcttt cgagcacgcg
      601 cagatcgacg aagctgtcgt cgatcgcaac gatgtcgaag acatcgtcga accggagacg
      661 gggcctgctg gtcggcggat cgttcaacgt gaacctcgtg cacaatcgtc gaatgcaaca
      721 tctctcacgg gcctctattt ccttcaattc aacagcaaat tatcggtcaa ttaaacgttc
      781 atccgttgaa tgcccaagtt aacgtccgtg gcaacgccga tcagccgtct gatttggcgc
      841 ccgtcagtcg ccatggacgc cggcgtgaat tccgttaggc tcggcggact tggttccagt
      901 caatgtcgag gcacgcccat gacacgaccc gatcggatgc cgccgatccc cttggaaaag
      961 ttaacccccg agcaaaagaa ggccgcggac gagatcgtct cgggtccgcg cggacgcgtt
     1021 gtcggtccct tcaacgtgct gctgcgcagt ccggatttca tgagccggtt gcagaagacg
     1081 ggcgagtacc tgcgcttcaa gagcgcacac gagccccggc tctccgagct cgcgatcctc
     1141 atcacggcac gccactggtc gcagaatttc gaatggcatc atcaccgccc gatcgccgag
     1201 aaggcgggac tgaaggcgga catcatcgat gcgatcgccg aggggcggag gcccgccacc
     1261 atggcgaagg acgaggcggt gatctacgac ctcctgaccg aactcaacgc gaacaagacc
     1321 gtgagcagtc cgacctatgc gcgtgcgctc ggccagttcg gcgagcgcgg catcgtcgat
     1381 ctcgtcggta tcgccggcta ctacagcctg ctcgccatgc tcatgaacac ggcggcgaca
     1441 ccgctgcccg aaggcacccc gccaccgctg ccgaccttac cctgacgctt ttgcgtgcgg
     1501 tcaacgcacc ggctcgcctg gcgcgtaagc gccgatggcg agcgcgtgca cgccctcggc
     1561 cagctcggcc ttcagtgcct cgttcaccat ccggtggcgt gcgacgcggg tttggccagc
     1621 gaaacggtcc gccacgacga gcacccgata gtggctttca ccggtcccgg gagagctcgc
     1681 gtggcccgca tgcatgtggg attcgttgac gacatcgagc cgcctcggct cgagtgccat
     1741 catcaaccgc tcgcgtatac gctcgtccat gctcatttgt gctacctgta ttgcgctcgg
     1801 atctgtcctg gcagggcctc gcggatttgg tacggacggg gtggcagcga tgaagtctgg
     1861 tgactttgca gcaacgccct gcacgaggct cgcataccgg acccggcaat ccttgtcgat
     1921 tggcggacga gacgacatac tcgcgacccc atgaagctcg actcgaaata tttcgacagc
     1981 cttcgctcca cggccgagcc tcgcagctcg cgccgcgagg cggctcacgc ttgccaatgg
     2041 gccggctgca acgagcccgc gtcgcaccgc gcgccgatgg ggcggggacg cgagggccgc
     2101 ttctatcgct tctgcctcga gcatgcgcgc cagttcaacg cgaactacaa ctacttccag
     2161 ggcatgagtg acaccgaggt cgagggatac ctcaaggatt ccgtcaccgg ccacaggcct
     2221 acttggcgca tgggcaacga cccggcactc gggcgcggcg cgcgctcagg cgcgtcgccg
     2281 cgctctcgcg acggtcgcct cgacggcgac gatgcccatg aggtcttcga ggatgcctac
     2341 cggggccggc cgggcg
//
LOCUS       DAORNH010000428         2210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_36869_length_2210_cov_2.461717, whole genome shotgun sequence.
ACCESSION   DAORNH010000428 DAORNH010000000
VERSION     DAORNH010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36869_length_2210_cov_2.461717"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..899)
                     /locus_tag="PK264_24655"
     CDS             complement(468..899)
                     /locus_tag="PK264_24655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRD79084.1"
                     /translation="MKRKGNGAGAVAFFHHRNHLKTFRLITDSAGTEVRRTTFRPFGD
                     KAAETGTHVEAKGYIGERHNAETGFLYLHARFYDPILGRFISSDWWDPNKPGVGTNRY
                     AYADNGRILEPKCCGLHETGSTSVPSSSCIGVSHLLGGNDP"
     gene            1215..>2210
                     /locus_tag="PK264_24660"
     CDS             1215..>2210
                     /locus_tag="PK264_24660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79085.1"
                     /translation="MLGQVGEVVLDELALSLHARGIRLRQRHGGVRVIVRLDLLAAVV
                     AAIGDGFELVDAENLLGLAAHVGKLCPIRAAIRHLVRNDEMVLSVDRNLHVVADDARA
                     APARRHRAAVGISQRDLLVGQGQHRLLVGFQFLHFSCQLREVQSIEERVDGANRIALV
                     DPIIEAFRQQRRLPALGPFHEPLHDPPPQIVRQFIPGGWCGASRRRRASSGSFGISVP
                     TSKSPASWASSGSSSSKASKVSSSSMIAAWLSAPSAMMPGENIMVASVNRLEPLVLDH
                     VQQLQRCASRLPLSALPSTERIGGDAQERGKHRLADPGPLAQGHDRRSVAPSPADA"
BASE COUNT          375 a          711 c          708 g          416 t
ORIGIN      
        1 tcgggccaga tccgcataaa atgacggcta ggacccccgg atcgtaccgg agcgcccgac
       61 gaaagctcgt caaacggtag cggagggtgg accacggatg agccaagcgg gcgatagccc
      121 gagacctgcg tgcgcgctga cttgtgtcac ccaggcgatc ggggcggcga tgaagtcgat
      181 cggcgcgatg cattctgtca atgacggggg cgcaattgcc accacaccaa cgccgaagca
      241 gagcgggcag gtatgggtcc gattggcggg cgcgtcatcg ggcagcgcgc ctgccgggcc
      301 ctcaggcatg gcgctgcaca gcatatcgat gcttgatggc tcgccggccc gtgtgacgcc
      361 gatcgcttgt gccgcagtca cgagcaggat gtgcaccgcc atcgccagca aggcgaggac
      421 gagcgccgac tgccgaactt tccgggcgcg catcatgcca tcgattttca gggatcgttg
      481 ccgccaagca aatgcgacac accgatgcag gaggacgatg gcactgaagt tgacccggtt
      541 tcgtggagcc cgcaacattt gggttccagg atacgaccat tgtccgcgta ggcgtagcgg
      601 ttcgtcccta cgcccggctt gttggggtcc caccagtcag agctgatgaa ccggccgagg
      661 atggggtcgt agaagcgggc gtgcaggtag aggaagcccg tctcggcgtt gtgccgctcg
      721 ccgatgtagc ccttggcctc gacatgtgtg ccggtctcgg cggccttgtc gccgaagggt
      781 cggaaggtcg ttcggcgcac ctccgtgccc gcgctatcgg tgatgagacg gaacgtcttt
      841 aggtgattcc ggtggtggaa gaacgccacc gcgccggcgc catttccctt ccgcttcacg
      901 tcgtcgtgga gatacttcgt ccacgtgcca tcgggcgcga tctcgacgcc tgagccgagg
      961 aaaaccgtga cgaggtccgc gccgccggcc ccgccgccgg ggacgaccct caatacgcgc
     1021 tcgttgtcgg cggcgtaggt gtagtggtgt gagcctctct gggattcccg tcgcggactc
     1081 aaccacctcc attccgcgga tgacttcacc tttggcgtct gacaggaccc ttccgtcttc
     1141 ggatgtgatg atgctccatc caccgtcgtg agccaatagg ccaacaactc gccggctgga
     1201 cagcgataga catcgtgctc ggccaagtag gcgaagtcgt gcttgacgaa cttgccctta
     1261 gcctccatgc ccgtggcatt cggcttcggc agcgtcacgg cggtgtccgc gtcatcgtac
     1321 gtcttgatct cctcgccgct gtagtagccg cgatcggcga cggcttcgag cttgtcgacg
     1381 ccgagaacct ccttggcttg gcggcccatg ttggcaagtt gtgcccgatc cgagccgcta
     1441 ttcgtcacct cgtgcgcaat gatgagatgg ttctcagtgt cgaccgcaac ctgcacgttg
     1501 tagccgacga cgcccgagcc gcgcccgctc gtcgccatcg agcggcagtc gggatcagtc
     1561 agcgagatct gctcgtcggg caaggccagc atcgccttct cgtgggcttc cagtttctgc
     1621 atttcagctg ccagcttcgc gaggttcaga gcatcgagga acgcgtcgac ggcgcgaaca
     1681 ggattgctct cgtcgaccca atcatcgagg cattccggca acagcgtcga ctgcccgcgc
     1741 tcggcccctt ccacgagccg cttcatgatc cgcccccgca aatcgtgagg caattcatac
     1801 caggcggatg gtgcggggcg tcacggcgac ggcgagcgtc cagtggttca ttcggaatct
     1861 ctgtccccac ctccaagtca ccggcgtcgt gggcatcgag cgggtcgtcg tcgtcgaagg
     1921 cgtcgaaggt gagcagctcg tcgatgatag cggcctggtt gtcggcgccg agcgcgatga
     1981 tgccaggcga gaacatcatg gtggcatcgg tgaaccgcct cgagcccctt gtccttgacc
     2041 acgtgcagca gcttcaacgc tgcgccagtc ggctgccgct ctccgcgctc ccatcgaccg
     2101 agcgtattgg tggcgacgct caggaacgcg gcaagcaccg cctggctgac cccggcccgc
     2161 tcgcgcaggg ccacgatcgc cgatctgtcg ctccatctcc tgctgatgct
//
LOCUS       DAORNH010000429         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_37101_length_2200_cov_2.396737, whole genome shotgun sequence.
ACCESSION   DAORNH010000429 DAORNH010000000
VERSION     DAORNH010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37101_length_2200_cov_2.396737"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PK264_24665"
     CDS             complement(<1..527)
                     /locus_tag="PK264_24665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79086.1"
                     /translation="MSNTVPDLGARTARPAWLLREGRVPERIVWAIAAIVALYYARLF
                     WLTEGLVPNILFTAGVSLAIASALTLLTRRLLFATTLTGAFIAIIYLTAATKRKIMFS
                     VVHAYDLVFYLSSWSTISYLWSDFRVYVLAFFGGVASLIIAGVVAWRLDSTRVDRRSA
                     ALAFLASALVGAYGA"
     gene            768..>1001
                     /locus_tag="PK264_24670"
     CDS             768..>1001
                     /locus_tag="PK264_24670"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRD79087.1"
                     /translation="MLSYRLEKFAAPLTAVKETAPVPVGRQVVVDITASGLCHSDLHL
                     HEGFFDLGDGARIDAKGIVNPPRTLGHEIVGIVD"
     assembly_gap    1002..1011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1012..>1354
                     /locus_tag="PK264_24675"
     CDS             <1012..>1354
                     /locus_tag="PK264_24675"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRD79088.1"
                     /translation="AVGPEASGAAVGDKRVVYPWIGCGTCHTCTSGDEQLCNAPQALG
                     VHRDGGFATKVLVPDTRYLVEYAPLPEAQACTYACSGLTAFGALKKLVPFPSGGRLLI
                     IGAGGVGLSGVR"
     assembly_gap    1355..1390
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1391..1831
                     /locus_tag="PK264_24680"
     CDS             <1391..1831
                     /locus_tag="PK264_24680"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRD79089.1"
                     /translation="VAEVDRTKWDVARAAGAADCVDPSAEGAARTLMKATGGGVAGAV
                     DFVGSGASFMFGFNALGKGGKLVSVGLFGGAARMAPAMLAMKAVTVTGSYVGSLAEMR
                     ELLAIARSGSIPELYVATRPLGEVNQAFGDLKEGRVKGRVVLLP"
     gene            1969..>2200
                     /locus_tag="PK264_24685"
     CDS             1969..>2200
                     /locus_tag="PK264_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRD79090.1"
                     /translation="MDTFDAGPRSGPPAPWLAALEARAPLELAVTLAMMPLLRLAPTG
                     DGHPVLVIPGLATSDMATITLRWYLSDCRYAAH"
BASE COUNT          373 a          695 c          747 g          339 t
ORIGIN      
        1 tcggccccgt aggcgccgac gagcgccgag gcgaggaagg cgagggcggc actccgccgg
       61 tcgacgcgcg tgctgtcgag ccgccaggca acgacgcccg cgatgatcag cgaggcgacg
      121 ccgccgaaga aggcaagcac atagacgcgg aagtcgctcc acaggtagct gatcgtcgac
      181 cacgacgaga gatagaagac gaggtcgtag gcatgcacga ccgagaacat gatcttccgc
      241 ttcgtcgccg ccgtcagata gatgatggcg atgaaggcgc ccgtcagggt cgtcgcaaac
      301 agcaggcgac gggtcaacag ggtgagcgcc gaggcgatcg caagcgacac gccggccgtg
      361 aaaaggatgt tcgggacgag gccctcggtc agccagaaga gccgcgcata atagagcgcg
      421 acaatcgcgg caatcgccca cacgatccgt tccggcacgc ggccttcgcg caagagccac
      481 gcgggcctcg ccgtccgggc gccgaggtct ggtactgtgt tgctcatgtc gggatgcggc
      541 tgctgaagct ctgctcgaaa acctgaaggc gacgcgcgct cgccaggcag taccgcgagc
      601 gtcatgaaac tgtcatcaca tacaccagcc cgattgccaa tgaacactcc ctgaatgccg
      661 ggagcgaggc gtcggctcgg ccggatgacg ggcacggctc ggcttgacgg ggcgcctgcg
      721 aaggcggaag gggagagcaa agcacatcct gtcgccccgg aggccccatg ctgagctacc
      781 gcctcgaaaa atttgccgcg ccgctgacgg ccgtgaagga gacggcgccg gtgccggtcg
      841 gccgccaggt ggtggtcgac atcacggcga gcggcctctg ccacagcgac ctgcacttgc
      901 acgaggggtt cttcgacctc ggtgacgggg ccaggatcga tgcgaagggc atcgtcaatc
      961 cgccgcgcac gctcgggcat gagatcgtcg gcatcgtcga tnnnnnnnnn ncagcggtcg
     1021 gccccgaggc cagcggcgcc gcagtcggcg acaagcgcgt cgtctatccg tggatcggct
     1081 gcggcacctg ccacacctgc acgtcgggcg acgagcagct ctgcaatgca ccgcaagcgc
     1141 tcggcgtgca ccgcgacggc ggctttgcaa cgaaagtgct cgtgcccgat acccgatacc
     1201 tcgtcgagta cgcgccgctt cccgaggcgc aggcctgcac gtatgcctgc tcgggcctta
     1261 cggcctttgg cgccttgaag aagcttgtcc cgtttccctc gggtgggcga ctgctcatca
     1321 tcggcgccgg cggcgtcggc ctatcgggcg tgcgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn gtcgccgagg tcgacaggac gaagtgggac gtcgccaggg ccgctggagc
     1441 cgccgattgc gtcgatccat cggcggaggg agcggcgcgg actctgatga aggcaaccgg
     1501 tgggggagtc gcgggtgcag tcgacttcgt cggctcgggc gcgagcttca tgttcggctt
     1561 taacgcgctt ggcaagggtg gcaagctcgt ctccgtcggc ctattcggag gggcggcccg
     1621 catggcgccc gccatgctcg ccatgaaggc tgtgaccgtg acgggctcct atgtgggcag
     1681 cctcgccgag atgcgcgaac tgctcgcgat cgcccggagt ggctcaattc ccgagctgta
     1741 tgtcgcgacc cgcccgctcg gcgaggtcaa ccaggccttc ggcgacctaa aggaggggcg
     1801 cgtcaaggga cgcgtggtgc tgctgcctta gcctaaggca gagatatgtg actgttgcgc
     1861 cacattcgag gaagcatctg gggccgcgtc ggcgtagtcc ctggcctcgt ggtggcggct
     1921 tcgccgaaca tgctgcgacc atatcgcact gctgccaatg aggcattctt ggatacgttc
     1981 gatgccggcc cacgctctgg accgccggcg ccctggcttg ctgcgctcga ggcccgcgcc
     2041 ccactggagc tggcggtgac gcttgccatg atgccgctgc tgcgtctcgc gccgacaggc
     2101 gacggacatc cggtgctcgt gatcccgggc cttgccacat ccgacatggc gacgatcacg
     2161 ctacgctggt atctgagcga ttgccgctat gcggcacacc
//
LOCUS       DAORNH010000430         2073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_39969_length_2073_cov_2.077800, whole genome shotgun sequence.
ACCESSION   DAORNH010000430 DAORNH010000000
VERSION     DAORNH010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39969_length_2073_cov_2.077800"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PK264_24690"
     CDS             <1..329
                     /locus_tag="PK264_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRD79091.1"
                     /translation="GAVELARRFEDAGVAAVIYTDIDRDGILTGLNLASTRELADATA
                     IPVIASGGLASIADIEALLAPENRKLAGAITGRALYDGRIDPAAALALLRGARRHTRS
                     VVSGPQ"
     gene            567..>690
                     /locus_tag="PK264_24695"
     CDS             567..>690
                     /locus_tag="PK264_24695"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD79092.1"
                     /translation="MSAKALRLYEERGLLKPLRTAAGWRAYGQEEMARATVVVEL"
     assembly_gap    691..700
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <701..1545
                     /locus_tag="PK264_24700"
     CDS             <701..1545
                     /locus_tag="PK264_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRD79093.1"
                     /translation="LSLAEVARVLGGDAPLLGRALAAHEAALEARARHLHDTVHKVRK
                     LRAELDRGTMSVANVVSSVRRAENQISVTFELPWPWGGEPFELRGLRGLTFITGPLGS
                     GKTRLAMRMAEKIPGASFVSEDRLIDGAAAAKEALAAQPELAARVRRSISEIVQAGGN
                     DCSALLALLTALEAEGATVHVVDMLESGLDEATQEALICFLRRRAPDARPLIALTRST
                     SILDVELVGPDEAIILCPANHSPPMPVAPVRGAPGYEALATCLAAPAVRARTEGVVAI
                     RRRA"
     gene            complement(1879..>2073)
                     /locus_tag="PK264_24705"
     CDS             complement(1879..>2073)
                     /locus_tag="PK264_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263865039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase-related (seleno)protein"
                     /protein_id="HRD79094.1"
                     /translation="QGEARAAVRRRLGDAALVDAVATFASFNAIVKIADGTGIPIEAW
                     KEERTRDFRATLGIETWDND"
BASE COUNT          383 a          683 c          623 g          374 t
ORIGIN      
        1 tcggcgcggt ggagctcgcg cgccgttttg aggacgccgg cgtcgccgcc gtcatctaca
       61 ccgacatcga ccgcgacggc atcctgaccg gcctcaacct cgcctcgacc cgcgaactcg
      121 ccgatgcgac cgcgatccct gtgatcgcct cgggcggcct cgcctcgatc gccgatatcg
      181 aggcgctgct cgcccccgaa aaccgcaagc ttgccggcgc gatcacgggc cgcgccctct
      241 acgacgggcg catcgacccg gctgcggcac tcgcgctgct gcgtggcgcc cgccgacaca
      301 cccgctcggt cgtgagtggc ccacaataga acaagccgtt ggctgatcct cctctgcgaa
      361 aggtggtctg ctctagatcg catcgcatcg cgtcgcagcc tcggctcggc gagttcctcc
      421 acaaaagatc gatcgtagga tcgatactcg cttgacccgt cccctgcggc atcctcgaaa
      481 agactggcgg tcagacaaca gcgaggtgat tcgtgaactc gaccgcccaa ctattgaacc
      541 cgtccgaggc cgccaagaaa ctcggcgtgt cggcaaaagc cctccgcctc tatgaggagc
      601 gaggcttgct caagccgttg cgaaccgccg ccggatggcg agcttacggg caagaagaaa
      661 tggcccgagc taccgtagtt gtcgaacttc nnnnnnnnnn gtttaagcct cgccgaagta
      721 gcgcgtgtcc ttggcggcga cgcgcccctg ctcgggcgcg ctctggcggc gcatgaagcc
      781 gcccttgaag cccgagcccg tcaccttcac gataccgtgc ataaagtgcg caaacttcgg
      841 gccgagctgg atcgcggcac catgtctgtt gccaatgtcg tatcgagtgt gcggcgagca
      901 gagaaccaga tcagcgtgac tttcgaactc ccgtggccgt ggggcggcga gccatttgaa
      961 ctgcggggat tacgcggcct aacgttcatt acgggacctc tggggagcgg aaaaacgcgc
     1021 cttgccatgc gcatggcgga gaaaataccc ggagcgtcat ttgtcagcga ggatcggttg
     1081 atagacggtg cggcggcagc gaaagaagcg ttggcagcgc aaccggagct ggcggccagg
     1141 gtccgccgct cgatatccga gattgtccaa gcgggtggga atgattgcag cgcgctcttg
     1201 gccttgctaa cggccttgga ggccgaaggc gcgaccgtgc acgtcgtaga catgctcgag
     1261 agtggattgg acgaggccac gcaagaagca ttgatctgct ttttgagacg ccgcgcaccg
     1321 gatgcgcgac ctctgatcgc cctcacgcgt tccacatcga tactggatgt cgaactggtc
     1381 ggcccagacg aagcaatcat tctgtgccca gcgaaccata gcccgccgat gcctgtcgct
     1441 ccggttcgcg gtgcacccgg ttatgaggcc ctggcgacgt gccttgccgc gcctgcagtt
     1501 cgagctcgta ccgaaggcgt agtcgccatc agacgccgcg cgtgaggttt gcgagaggct
     1561 cggccaaatt ccacgccaag ccttccgctc ctcatccgaa agctgccgaa attttcgatt
     1621 cgtcgacgtc cagaagagag ttctcgtgtt gcccccaagc cgacattgcg tccactactt
     1681 gtgcacgcac gaaaccgact gcatgtcccc gcaaggccgt tggacaaccc gttctcacct
     1741 tgcagctgaa agatacgagc ttgcgaacat tgcgccacac caagtgctcg gcaatcttgc
     1801 tgcccctatt gatcgcgacg tcccgctgat tttggatggc aaagggcttc gcgatcagga
     1861 cacgagcctc gcgccgcctc agtcgttgtc ccaggtctcg atgccaagcg tcgcccggaa
     1921 atcgcgcgtg cgctcctcct tccaggcctc gatcgggatt ccggtgccgt cggcgatctt
     1981 cacgatcgca ttgaaggacg cgaacgtcgc aacggcatcg acgagggccg catcgccgag
     2041 cctccgtcgc acggccgcgc gcgcctcgcc ctg
//
LOCUS       DAORNH010000431         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_40161_length_2065_cov_2.110945, whole genome shotgun sequence.
ACCESSION   DAORNH010000431 DAORNH010000000
VERSION     DAORNH010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40161_length_2065_cov_2.110945"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..>301)
                     /locus_tag="PK264_24710"
     CDS             complement(106..>301)
                     /locus_tag="PK264_24710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79095.1"
                     /translation="PSIMKLMVRDGVVEMTITAPKIVETNGKSDGKTVKFVWPWTEFA
                     TLLVKADARKGKTYFAVVEY"
     assembly_gap    302..320
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<321..944)
                     /locus_tag="PK264_24715"
     CDS             complement(<321..944)
                     /locus_tag="PK264_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79096.1"
                     /translation="MLPIDDAAPCAGTGPVAMLRRARGRIAGAMLAAMIATGAAGCLD
                     IDAHLQLKGDGNSAVVADVRLDQEMQHVFAFVELLGQQVPQGAAFLRRGLCPAVEFLT
                     AFALGGAFDVQGKQAVVKDKFVCSFSADLGTTEAVMGNLGPNSMAGSMGVFVVKPEGP
                     KRYRVTLNLAVLPDLSAMATMQLSNDYMKKEGKMPDPAAMERFQRALE"
     gene            complement(1109..>2065)
                     /locus_tag="PK264_24720"
     CDS             complement(1109..>2065)
                     /locus_tag="PK264_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79097.1"
                     /translation="ALDHGHFGVKLHRSTGSGWQEIAAPAYPPKPDSEPEIDPMGKTV
                     PWSVVKIWSLEAGGAAEPGVLWCGTIPGGLFKSMDAGQTWALNTALWNDPRRKKWFGG
                     GADYAGIHSIAVDPRDHRTVRVAASCGGVWRTTDSGVSWACTADGMKAAYMPPDVAND
                     PGVQDAHRLVQAPSRPDRLWVQHHDTIYRSDDAGGIWHDIGKVNPSSFGFAVAVHPND
                     PDRAWFVPAVKDEKRYPVDGRLVVTRTSDGGRTFEALSKGLPQEHAYDLIYRHGLDVD
                     ETGRRLVIGSTTGALFISEDQGGSWTMVSAHLPPIHCVRFVH"
BASE COUNT          348 a          687 c          657 g          354 t
ORIGIN      
        1 ggctcccgcc tgttcagatc gaaacatgcg tgtctccgta tctcggttcg gccgactgca
       61 gctcatggct ggcggccagg atggatcgat gagcactcgg cctgatcagt attcgaccac
      121 ggcgaagtag gtcttgccct tgcgcgcgtc agccttgacg aggagggtcg cgaattccgt
      181 ccagggccag acgaacttga cggtcttgcc gtccgacttg ccgttcgtct cgacaatctt
      241 cggcgccgtg atcgtcatct cgacgacacc gtcgcgcacc atcagcttca tgatgctcgg
      301 cnnnnnnnnn nnnnnnnnnn ctcaagcgcc cgctggaagc gctccatggc ggccggatcg
      361 ggcatcttgc cttccttttt catgtagtcg ttcgacagct gcatcgtcgc catcgccgac
      421 aggtcgggca ggacggcgag gttcagcgtc acgcggtaac gcttcggccc ctcgggcttc
      481 acgacgaaca cgcccatgga gccggccatg ctgttcggcc cgaggttgcc catgacggct
      541 tccgtggtgc cgaggtcggc cgagaagctg cagacgaact tgtccttgac gactgcctgc
      601 ttgccctgca cgtcgaaggc accgccgagc gcaaaggcag tcaaaaactc gaccgcgggg
      661 catagaccgc gtcgcagaaa ggctgcgccc tgcggcacct gctggccgag cagctcgacg
      721 aaggcgaaga cgtgctgcat ctcctggtcg aggcgcacat cggctaccac agccgagttg
      781 ccgtcacctt tcagttgcag atgagcatcg atgtcgaggc agcccgcggc gccggtggcg
      841 atcatggccg ccagcatcgc tcccgcgatc cgcccgcgtg ctcgtcgcag catcgcaacc
      901 ggtccagtgc cggcgcaggg cgcggcatcg tcaatcggca gcatgcagcc tcctctttcc
      961 tcaagggtcg caatgggcag tggtgcccga aatccgcagg tgccacgcgc tccgcgcgcg
     1021 cacatcgggg cgcagaccgg cctcgcccag cctgagcttt ggcagaaggg ttcggccgaa
     1081 gcaaggccgc gcgtgtcacg caatcgtttc agtgcacaaa tcgtacacaa tggatcggcg
     1141 gcagatgcgc cgacaccatc gtccagctgc caccttggtc ctccgagatg aacaaggctc
     1201 cagtcgtgct accgatcacg aggcggcggc ccgtctcgtc gacgtcgagg ccgtggcggt
     1261 agatgagatc gtaggcgtgc tcctggggaa gccccttcga gagtgcctcg aacgtgcgcc
     1321 cgccatcgct cgtgcgcgtg acgacgaggc ggccgtcgac cggatagcgc ttctcgtcct
     1381 tgaccgccgg gacgaaccag gcgcgatccg gatcgttcgg gtggacggcc acagcgaatc
     1441 cgaacgacga gggattgacc ttgccgatgt catgccaaat gccgcctgca tcgtcagagc
     1501 gatagatcgt atcgtgatgc tggacccaga gccggtcggg ccggctcggc gcctggacga
     1561 ggcggtgggc atcctggaca cccgggtcgt tggcgacatc gggcggcatg taggcggcct
     1621 tcatgccgtc ggccgtgcac gcccagctca cgccgctgtc ggtcgtccgc cagacgccgc
     1681 cacacgatgc agcgacgcgc acggtgcgat gatcgcgcgg atcgacggcg atcgagtgta
     1741 tgccggcata gtccgccccg cctccgaacc acttcttgcg gcgcggatcg ttccaaagcg
     1801 cagtattgag ggcccatgtc tgtccggcgt ccatcgactt gaagaggccg cccggaatgg
     1861 tgccgcacca cagcacaccc ggctcggcgg ccccgcccgc ctcgagtgac cagatcttca
     1921 cgaccgacca gggcacggtc ttgcccatcg gatcaatctc cggctcgctg tccggcttcg
     1981 gcggataggc gggagcggcg atctcctgcc agcccgatcc ggtcgagcga tggagcttga
     2041 cgccgaaatg accgtggtca agcgc
//
LOCUS       DAORNH010000432         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_44023_length_1914_cov_3.772458, whole genome shotgun sequence.
ACCESSION   DAORNH010000432 DAORNH010000000
VERSION     DAORNH010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44023_length_1914_cov_3.772458"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK264_24725"
     CDS             complement(<1..226)
                     /locus_tag="PK264_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HRD79098.1"
                     /translation="MNETGTHSTGRCPVTHGGVTSAHMANVDWWPRALNLDILHQHDN
                     KTNPLGASFDYLEQLKKLDVDALKKDVKALM"
     gene            complement(729..908)
                     /locus_tag="PK264_24730"
     CDS             complement(729..908)
                     /locus_tag="PK264_24730"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRD79099.1"
                     /translation="MIRHPLIGRPVECDLRELLISRGRSGYVALYSLEEDHQAVLILA
                     TRHQREAGYWPQGEE"
     gene            complement(1123..1320)
                     /locus_tag="PK264_24735"
     CDS             complement(1123..1320)
                     /locus_tag="PK264_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79100.1"
                     /translation="MPTISVKLSDKIKQRVVAAAERQGVSPHAFMVHAIEQAAALAER
                     RASFVSEARAARDQMLRTGQG"
     gene            complement(1427..>1914)
                     /locus_tag="PK264_24740"
     CDS             complement(1427..>1914)
                     /locus_tag="PK264_24740"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HRD79101.1"
                     /translation="MNERAARADILARVRAALRHDPAHAEQRRARLRQTIAMPTEGPR
                     PSLVGDLVGITGASCAIAETGTLMLLSGTRTASTTRLLPETHIAMVAAGRIVADMEEG
                     FALLRHERGEGSKLPRAVNFVSGPSRTGDIEQTVVLGAHGPYRVHLLVVGTTADSPMA
                     A"
BASE COUNT          328 a          587 c          623 g          376 t
ORIGIN      
        1 tcatcagcgc cttgacgtcc ttcttgagcg cgtcgacgtc aagcttcttg agttgctcga
       61 gatagtcgaa gctggcgccc agtggattgg tcttgttatc gtgctggtgc aggatgtcga
      121 ggttcaaggc cctgggccac cagtcgacgt tggccatgtg ggctgaagtt acgccgccgt
      181 gcgtgaccgg gcatctgccc gttgagtgtg tgccagtttc gttcatggtt atctccgatc
      241 ggcttgggtc atccgcgatg acatgagttc atactatggc gcgatggtta caagataaag
      301 ttgattgtga caatgacggc tataggtaat agctatcgca tgtctgccaa tagttcgtaa
      361 cttgaaacgg tccgccgtct aatgaccaag gaacaatcaa cccgcagtgc gtcgccacgg
      421 gccaatgaag ccaacgacaa ggttctgagc catggcagcc atttgattgt cccgcatacg
      481 gccatggtga gcggctggtg gtggccgatc gcgatgcgaa aacgatcgag ctggattgcg
      541 tcgcacaggg ctgccgcagg cggtcgacca ggctgctcaa ctcttgccag gcaaggttcc
      601 cggatcagcg cgcatcgcgt tgacccagaa agcttcgccg gcgaagaccg atccgctcgc
      661 gacgccgatc gccatggcgc cggacggtgt cgcctgcaat tgcctgaatc gccacgatgc
      721 acaagcactc attcttcgcc ctgcggccaa tatccagctt cacgctgatg gcgggtcgca
      781 agaatcagga cggcctggtg atcctcctcg aggctgtaga gcgccacgta gccgctacgg
      841 ccacgggaga tgagcaactc gcgcaagtca cactcgacgg ggcgaccgat cagcgggtga
      901 cgaatcaatg gatctaccgc ttccgcaatc aactcatatt ggcacagggg gccgttgcag
      961 ctgctgcgcc aggatcaatc tcgatcagga aatcggtcgc tctttcgaga tcggcaagcg
     1021 cccgctccga gtagatcagc cttgccagga tcctgccttc ggctttgacg gttgcccccc
     1081 ggcaatgcgt gccttcaggt aggcgtgaac ttcgctggca tcttagcctt ggccggtcct
     1141 cagcatctgg tcccgcgctg ctcgcgcctc ggacacgaag ctcgcccgtc gttctgccag
     1201 agcagcggcc tgctctatcg cgtgcaccat gaacgcatgt ggcgaaacgc cttgcctctc
     1261 ggcagcggca acgacccgct gcttgatctt gtcggaaagc ttcactgaga tggtgggcat
     1321 ggtcgggatc ctcgtgcctg ggcgtcgcgc aaaagcaaca gcgccgcggc cggccttgcg
     1381 ggtcggcatt cttgcccggt gcgtccgcgc cggtgatggc cggatatcag gccgccatcg
     1441 gtgagtcggc agtggtgccg acgaccagca ggtgcacccg atacggccca tgtgcgccga
     1501 gcacgacggt ctgctcgatg tcaccggtgc gcgagggtcc ggagacgaag tttacggcgc
     1561 gcggcagttt gctgccttcg ccgcgttcat ggcggagcag cgcgaagccc tcctccatgt
     1621 cggcgacgat gcgtccggcg gccaccatgg cgatgtgcgt ttcgggcagc aggcgggtcg
     1681 tcgacgcggt ccgtgtgccg gagagcagca tcagcgtgcc ggtctcggcg atcgcgcagg
     1741 aggcgccggt gatgccgacc aggtcgccga ccaagctcgg gcgcgggcct tcggtcggca
     1801 tcgcgatggt ctggcgcagc cgcgcccggc gttgctcggc gtgcgccggg tcgtgccgga
     1861 gggcggcgcg aacgcgcgcc aggatgtctg ctcgcgctgc gcgctcgttc atgc
//
LOCUS       DAORNH010000433         1853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate AMR_MDS_5452
            NODE_45758_length_1853_cov_1.959399, whole genome shotgun sequence.
ACCESSION   DAORNH010000433 DAORNH010000000
VERSION     DAORNH010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867669
            Sequence Read Archive: SRR1051195
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (wastewater metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:57:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,952
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,795
            CDSs (with protein)               :: 4,795
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 63 of 115
            Pseudo Genes (incomplete)         :: 51 of 115
            Pseudo Genes (internal stop)      :: 6 of 115
            Pseudo Genes (multiple problems)  :: 5 of 115
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45758_length_1853_cov_1.959399"
                     /isolate="AMR_MDS_5452"
                     /isolation_source="Domestic wastewater treatment plant
                     (WWTP)"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /geo_loc_name="Australia"
                     /lat_lon="27.49 S 153.19 E"
                     /collection_date="2008-08-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK264_24745"
     CDS             complement(<1..506)
                     /locus_tag="PK264_24745"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRD79102.1"
                     /translation="MAKIDYYMSLNSPWTYLGSKRFAEMAKKYGHTVDIKPAKFGEVF
                     AKTGGLPLPQRSPERRAYRLMELKRWREETGLPITIQPKHFPSDEGPGTRLVIAAKLQ
                     GKDAHALATEIGKALWELEGNIGEDAVLDAAAKRAGLDAAAIRKSAPPAAELDALHAR
                     YTAEALAAG"
     gene            complement(516..>1083)
                     /locus_tag="PK264_24750"
     CDS             complement(516..>1083)
                     /locus_tag="PK264_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRD79103.1"
                     /translation="AAAAGLGSLDSPVSGGVNGAEAATLTLMVGGKAEAVAKARPALE
                     AMGKRVVHCGEAGAGQAAKICNNMMLAINMLGACEAFVLAEKLGLSHKALFDVASTSS
                     GQSWALTSYCPVPELVPTTPANSGYRPGFASALMLKDLKLSQEASQTAGATTPMGALA
                     AQLFGLHNAWGEGGADFTGIIHLIRGKS"
     assembly_gap    1084..1093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1094..1442)
                     /locus_tag="PK264_24755"
     CDS             complement(<1094..1442)
                     /locus_tag="PK264_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HRD79104.1"
                     /translation="MAKVGFIGLGNMGVPMAGNLVKKGHQVQGFDLVAGNLEKAKARG
                     VATVASAADAAKGADIVVTMLPAGKDTIAVYEKSGILQAAPKGAILVDAFSRTNMPHI
                     YAVGDVTGPPMLAH"
     gene            complement(1557..>1853)
                     /locus_tag="PK264_24760"
     CDS             complement(1557..>1853)
                     /locus_tag="PK264_24760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRD79105.1"
                     /translation="MLVNDRSLIPRAIEEGVRVFRTLPSHNRVIINPEAKASLSLGFE
                     DNRTTSHNYNYNGTSPGSSSSTSGSAPVSGTSPGIAYPLPPVDFKGVPARGGRH"
BASE COUNT          262 a          625 c          640 g          316 t
ORIGIN      
        1 ccggccgcca gcgcttccgc ggtatagcgg gcgtgcagcg cgtcgagctc ggctgccggc
       61 ggcgccgact tgcggatcgc ggcggcgtcg agaccggcac gcttcgccgc ggcatcgagc
      121 accgcatcct cgccgatgtt gccctcgagc tcccagagcg ccttgccgat ctccgtcgca
      181 agcgcgtgcg catccttccc ctggagcttc gccgcgatca cgagacgcgt gcccggaccc
      241 tcgtcggagg gaaagtgctt cggttgtatg gtgatcggca gccccgtctc ctcgcgccag
      301 cgcttcagct ccatgagccg ataggcgcgc cgctcgggcg agcgctgcgg cagggggagc
      361 ccgccggtct tcgcgaacac ctcgccgaac ttcgccggct tgatgtcgac cgtgtgcccg
      421 tacttcttcg ccatctccgc gaagcgcttg ctgccaaggt aggtccaggg cgagttcaag
      481 gacatgtagt agtcgatctt ggccatgacg ggctctcagc tcttgccgcg gatgagatgg
      541 atgataccgg tgaagtcggc gccgccctcg ccccaggcgt tgtggaggcc gaagagctgg
      601 gctgcgagcg cgcccatcgg cgtcgtcgcg ccggccgtct gtgaggcctc ctgcgagagc
      661 ttcaggtcct tcagcatgag ggcggaggcg aagcccggcc tgtagcccga attcgcgggc
      721 gtcgtcggca cgagctccgg cacaggacag tagctcgtca gcgcccagct ctgcccggac
      781 gaggtcgagg cgacgtcgaa cagcgccttg tgcgagaggc cgagcttctc cgcgagcacg
      841 aaagcctcgc aggcgccgag catgttgatc gccagcatca tgttgttgca gattttggcg
      901 gcctgcccgg cgccggcctc gccgcaatgg acgacgcgct tgcccatggc ctcgagcgcg
      961 ggccgggcct tggcgaccgc ctcggccttg ccgccgacca tcagcgtgag cgttgccgcc
     1021 tccgcgccat tgacgccacc cgagacgggg ctgtcgagcg agccgagccc ggccgcggcg
     1081 gcannnnnnn nnntgtgcgc cagcatcggc gggccggtca cgtcgccgac ggcgtagatg
     1141 tgcggcatgt tggtgcggct gaaggcgtcg accaggatcg cgcccttcgg cgccgcttgc
     1201 agaatgccgc tcttctcgta gacggcgatc gtgtccttgc ccgccggcag catggtgacg
     1261 acgatgtcgg cgcccttggc ggcatccgcg gcgctggcga cggtcgcgac gccgcgggcc
     1321 ttggccttct cgaggtttcc ggcgacgaga tcgaagccct ggacctggtg ccccttcttc
     1381 acgagattgc ccgccatggg tacgcccatg ttgccgagcc cgatgaagcc aaccttcgcc
     1441 attgcgtcct cctccgtgcc tcaaacgcgg atgccccgca cgagtgtacg aggcacctta
     1501 ggctcgaagg ttcagcgcgc gcaattgcta cgcgcgcggt cgtgatgcgc gtcgtatcag
     1561 tggcggccgc cgcgagcggg tacgcccttg aagtcgaccg gcgggagcgg ataggcgatg
     1621 cccgggctcg tccccgacac cggcgcggag cccgacgtgg aggacgagga gcccggcgac
     1681 gttccattgt agttgtagtt gtgcgaggtc gtgcggttgt cctcgaagcc gaggctcaaa
     1741 ctcgccttcg cctccggatt gatgatcaca cggttgtggc tcggcagcgt gcggaagacg
     1801 cgcacgccct cctcgatggc gcgggggatg agcgaacggt cgttcacgag cat
//
